LOCUS       DBFIFI010000001        80249 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.001, whole genome shotgun sequence.
ACCESSION   DBFIFI010000001 DBFIFI010000000
VERSION     DBFIFI010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 80249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80249
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.001"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..2294)
                     /gene="pbpC"
                     /locus_tag="N7B39_000001"
     CDS             complement(<1..2294)
                     /gene="pbpC"
                     /locus_tag="N7B39_000001"
                     /EC_number="2.4.99.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311412.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HGU2420073.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEVA
                     RNKVLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEALPLPDGALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGEIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIASV"
     gene            complement(2295..7256)
                     /gene="yfhM"
                     /locus_tag="N7B39_000002"
     CDS             complement(2295..7256)
                     /gene="yfhM"
                     /locus_tag="N7B39_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HGU2420074.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSAPERQKLAQQSAGKALTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPNQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPNDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKAGYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADEFGSNESKVIVAAPVIAELNMPRF
                     MASGDNSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRDEEAIALALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYSLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKAQNSNLNSDQLATLQVTNSGEQPLWLRVDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            7463..8308
                     /gene="sseA"
                     /locus_tag="N7B39_000003"
     CDS             7463..8308
                     /gene="sseA"
                     /locus_tag="N7B39_000003"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HGU2420075.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPLK"
     gene            8651..8776
                     /gene="timP"
                     /locus_tag="N7B39_000004"
     CDS             8651..8776
                     /gene="timP"
                     /locus_tag="N7B39_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HGU2420076.1"
                     /translation="MKIRCFCIVLIVSGALLTEVNNNRSLSGDNLLVINNLQSSR"
     gene            complement(8808..9584)
                     /gene="sseB"
                     /locus_tag="N7B39_000005"
     CDS             complement(8808..9584)
                     /gene="sseB"
                     /locus_tag="N7B39_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HGU2420077.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQVAGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(9727..11010)
                     /gene="pepB"
                     /locus_tag="N7B39_000006"
     CDS             complement(9727..11010)
                     /gene="pepB"
                     /locus_tag="N7B39_000006"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HGU2420078.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVASDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(11188..11388)
                     /gene="iscX"
                     /locus_tag="N7B39_000007"
     CDS             complement(11188..11388)
                     /gene="iscX"
                     /locus_tag="N7B39_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HGU2420079.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(11400..11735)
                     /gene="fdx"
                     /locus_tag="N7B39_000008"
     CDS             complement(11400..11735)
                     /gene="fdx"
                     /locus_tag="N7B39_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HGU2420080.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(11737..13587)
                     /gene="hscA"
                     /locus_tag="N7B39_000009"
     CDS             complement(11737..13587)
                     /gene="hscA"
                     /locus_tag="N7B39_000009"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HGU2420081.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDIEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(13604..14119)
                     /gene="hscB"
                     /locus_tag="N7B39_000010"
     CDS             complement(13604..14119)
                     /gene="hscB"
                     /locus_tag="N7B39_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HGU2420082.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(14215..14538)
                     /gene="iscA"
                     /locus_tag="N7B39_000011"
     CDS             complement(14215..14538)
                     /gene="iscA"
                     /locus_tag="N7B39_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HGU2420083.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(14555..14941)
                     /gene="iscU"
                     /locus_tag="N7B39_000012"
     CDS             complement(14555..14941)
                     /gene="iscU"
                     /locus_tag="N7B39_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HGU2420084.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(14969..16183)
                     /gene="iscS"
                     /locus_tag="N7B39_000013"
     CDS             complement(14969..16183)
                     /gene="iscS"
                     /locus_tag="N7B39_000013"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HGU2420085.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(16295..16783)
                     /gene="iscR"
                     /locus_tag="N7B39_000014"
     CDS             complement(16295..16783)
                     /gene="iscR"
                     /locus_tag="N7B39_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HGU2420086.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(17084..17821)
                     /gene="trmJ"
                     /locus_tag="N7B39_000015"
     CDS             complement(17084..17821)
                     /gene="trmJ"
                     /locus_tag="N7B39_000015"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HGU2420087.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            17940..18743
                     /gene="suhB"
                     /locus_tag="N7B39_000016"
     CDS             17940..18743
                     /gene="suhB"
                     /locus_tag="N7B39_000016"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HGU2420088.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            18888..19742
                     /gene="yfhR"
                     /locus_tag="N7B39_000017"
     CDS             18888..19742
                     /gene="yfhR"
                     /locus_tag="N7B39_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU2420089.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVNFI
                     LSALNPQN"
     gene            19933..21213
                     /gene="csiE"
                     /locus_tag="N7B39_000018"
     CDS             19933..21213
                     /gene="csiE"
                     /locus_tag="N7B39_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HGU2420090.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(21205..22344)
                     /gene="hcaT"
                     /locus_tag="N7B39_000019"
     CDS             complement(21205..22344)
                     /gene="hcaT"
                     /locus_tag="N7B39_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HGU2420091.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(22504..23394)
                     /gene="hcaR"
                     /locus_tag="N7B39_000020"
     CDS             complement(22504..23394)
                     /gene="hcaR"
                     /locus_tag="N7B39_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HGU2420092.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            23530..24891
                     /gene="hcaE"
                     /locus_tag="N7B39_000021"
     CDS             23530..24891
                     /gene="hcaE"
                     /locus_tag="N7B39_000021"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HGU2420093.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            24888..25406
                     /gene="hcaF"
                     /locus_tag="N7B39_000022"
     CDS             24888..25406
                     /gene="hcaF"
                     /locus_tag="N7B39_000022"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HGU2420094.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEICYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            25406..25726
                     /gene="hcaC"
                     /locus_tag="N7B39_000023"
     CDS             25406..25726
                     /gene="hcaC"
                     /locus_tag="N7B39_000023"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HGU2420095.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            25723..26535
                     /gene="hcaB"
                     /locus_tag="N7B39_000024"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             25723..26535
                     /gene="hcaB"
                     /locus_tag="N7B39_000024"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HGU2420096.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIAGEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            26545..27747
                     /gene="hcaD"
                     /locus_tag="N7B39_000025"
     CDS             26545..27747
                     /gene="hcaD"
                     /locus_tag="N7B39_000025"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HGU2420097.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDEQHLPYER
                     PPLSKSMLLEDSPQLQSVLPAHWWQENNVHLHSGVTIKTLGRDTRELVLANGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANDQLAREANLDTTNGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNHAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRSIRKWIQSGKTFDAKQLTD
                     ENIALKSL"
     gene            27844..28266
                     /gene="yphA"
                     /locus_tag="N7B39_000026"
     CDS             27844..28266
                     /gene="yphA"
                     /locus_tag="N7B39_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HGU2420098.1"
                     /translation="MNSLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(28314..29186)
                     /gene="yphB"
                     /locus_tag="N7B39_000027"
     CDS             complement(28314..29186)
                     /gene="yphB"
                     /locus_tag="N7B39_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HGU2420099.1"
                     /translation="MTIYTLSHGSLKLGVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIIETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(29198..30292)
                     /gene="yphC"
                     /locus_tag="N7B39_000028"
                     /pseudo
     CDS             complement(29198..30292)
                     /gene="yphC"
                     /locus_tag="N7B39_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
     gene            complement(30325..31323)
                     /gene="yphD"
                     /locus_tag="N7B39_000029"
     CDS             complement(30325..31323)
                     /gene="yphD"
                     /locus_tag="N7B39_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU2420100.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSHHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(31348..32859)
                     /gene="yphE"
                     /locus_tag="N7B39_000030"
     CDS             complement(31348..32859)
                     /gene="yphE"
                     /locus_tag="N7B39_000030"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HGU2420101.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGEIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAHKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVIRAVKKMSALGVAVIYVSHRMEEIRRIASS
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHAPVNVDELMSAILSVH"
     gene            complement(32882..33865)
                     /gene="yphF"
                     /locus_tag="N7B39_000031"
     CDS             complement(32882..33865)
                     /gene="yphF"
                     /locus_tag="N7B39_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HGU2420102.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(33962..37243)
                     /gene="yphG"
                     /locus_tag="N7B39_000032"
     CDS             complement(33962..37243)
                     /gene="yphG"
                     /locus_tag="N7B39_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HGU2420103.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTRLDAYEE
                     KRFEQFFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCD
                     ALLDDAVALTPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLPDVAKA
                     PLSAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPINQEVLCLHNLLLVLSGRQDNARL
                     QREKLLRDYPLNATLWCLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNEAIALWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGTTPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICAKAQGDETEATRCLHLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQHTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLAAAGLGEIAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            37361..38554
                     /gene="yphH"
                     /locus_tag="N7B39_000033"
     CDS             37361..38554
                     /gene="yphH"
                     /locus_tag="N7B39_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HGU2420104.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGEHWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVET
                     QLRQI"
     gene            complement(38617..39870)
                     /gene="glyA"
                     /locus_tag="N7B39_000034"
     CDS             complement(38617..39870)
                     /gene="glyA"
                     /locus_tag="N7B39_000034"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HGU2420105.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEVEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            40198..41388
                     /gene="hmpA"
                     /locus_tag="N7B39_000035"
     CDS             40198..41388
                     /gene="hmpA"
                     /locus_tag="N7B39_000035"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HGU2420106.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFAAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(41433..41771)
                     /gene="glnB"
                     /locus_tag="N7B39_000036"
     CDS             complement(41433..41771)
                     /gene="glnB"
                     /locus_tag="N7B39_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HGU2420107.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(41832..43166)
                     /gene="glrR"
                     /locus_tag="N7B39_000037"
     CDS             complement(41832..43166)
                     /gene="glrR"
                     /locus_tag="N7B39_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HGU2420108.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(43156..43869)
                     /gene="qseG"
                     /locus_tag="N7B39_000038"
     CDS             complement(43156..43869)
                     /gene="qseG"
                     /locus_tag="N7B39_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HGU2420109.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(44034..45461)
                     /gene="qseE"
                     /locus_tag="N7B39_000039"
     CDS             complement(44034..45461)
                     /gene="qseE"
                     /locus_tag="N7B39_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HGU2420110.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELDNVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(46037..49924)
                     /gene="purL"
                     /locus_tag="N7B39_000040"
                     /gene_synonym="purI"
     CDS             complement(46037..49924)
                     /gene="purL"
                     /locus_tag="N7B39_000040"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HGU2420111.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIATLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            50181..51737
                     /gene="mltF"
                     /locus_tag="N7B39_000041"
     CDS             50181..51737
                     /gene="mltF"
                     /locus_tag="N7B39_000041"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HGU2420112.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHMQ
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(51734..52237)
                     /gene="tadA"
                     /locus_tag="N7B39_000042"
     CDS             complement(51734..52237)
                     /gene="tadA"
                     /locus_tag="N7B39_000042"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HGU2420113.1"
                     /translation="MSEVEFSHEYWMRHAMTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(52295..52930)
                     /gene="yfhb"
                     /locus_tag="N7B39_000043"
     CDS             complement(52295..52930)
                     /gene="yfhb"
                     /locus_tag="N7B39_000043"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HGU2420114.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQALQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            53139..53987
                     /gene="yfhH"
                     /locus_tag="N7B39_000044"
     CDS             53139..53987
                     /gene="yfhH"
                     /locus_tag="N7B39_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HGU2420115.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            54043..54303
                     /gene="yfhL"
                     /locus_tag="N7B39_000045"
     CDS             54043..54303
                     /gene="yfhL"
                     /locus_tag="N7B39_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HGU2420116.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPQHVETEEQLWDKFVLMHHADKI"
     gene            complement(54497..54577)
                     /gene="shoB"
                     /locus_tag="N7B39_000046"
     CDS             complement(54497..54577)
                     /gene="shoB"
                     /locus_tag="N7B39_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HGU2420117.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(54999..55379)
                     /gene="acpS"
                     /locus_tag="N7B39_000047"
     CDS             complement(54999..55379)
                     /gene="acpS"
                     /locus_tag="N7B39_000047"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HGU2420118.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWEIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVANMHVTLADERHYACATVIIES"
     gene            complement(55379..56110)
                     /gene="pdxJ"
                     /locus_tag="N7B39_000048"
     CDS             complement(55379..56110)
                     /gene="pdxJ"
                     /locus_tag="N7B39_000048"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HGU2420119.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(56122..56850)
                     /gene="recO"
                     /locus_tag="N7B39_000049"
     CDS             complement(56122..56850)
                     /gene="recO"
                     /locus_tag="N7B39_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HGU2420120.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGVTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(56862..57767)
                     /gene="era"
                     /locus_tag="N7B39_000050"
     CDS             complement(56862..57767)
                     /gene="era"
                     /locus_tag="N7B39_000050"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HGU2420121.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVATIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(57764..58444)
                     /gene="rnc"
                     /locus_tag="N7B39_000051"
     CDS             complement(57764..58444)
                     /gene="rnc"
                     /locus_tag="N7B39_000051"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HGU2420122.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(58717..59691)
                     /gene="lepB"
                     /locus_tag="N7B39_000052"
     CDS             complement(58717..59691)
                     /gene="lepB"
                     /locus_tag="N7B39_000052"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HGU2420123.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(59707..61506)
                     /gene="lepA"
                     /locus_tag="N7B39_000053"
     CDS             complement(59707..61506)
                     /gene="lepA"
                     /locus_tag="N7B39_000053"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HGU2420124.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(61704..62183)
                     /gene="rseC"
                     /locus_tag="N7B39_000054"
     CDS             complement(61704..62183)
                     /gene="rseC"
                     /locus_tag="N7B39_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HGU2420125.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(62180..63136)
                     /gene="rseB"
                     /locus_tag="N7B39_000055"
     CDS             complement(62180..63136)
                     /gene="rseB"
                     /locus_tag="N7B39_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HGU2420126.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(63136..63774)
                     /gene="rseA"
                     /locus_tag="N7B39_000056"
     CDS             complement(63136..63774)
                     /gene="rseA"
                     /locus_tag="N7B39_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HGU2420127.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHEWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(63807..64382)
                     /gene="rpoE"
                     /locus_tag="N7B39_000057"
     CDS             complement(63807..64382)
                     /gene="rpoE"
                     /locus_tag="N7B39_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HGU2420128.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(64379..64534)
                     /gene="rseD"
                     /locus_tag="N7B39_000058"
     CDS             complement(64379..64534)
                     /gene="rseD"
                     /locus_tag="N7B39_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HGU2420129.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            64790..66412
                     /gene="nadB"
                     /locus_tag="N7B39_000059"
     CDS             64790..66412
                     /gene="nadB"
                     /locus_tag="N7B39_000059"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HGU2420130.1"
                     /translation="MNTLLEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHIQPNGEESYHLTREGGHSHRRILHAADATGREVETTLVSKALNHPNIRVLERS
                     NAVDLIISDKIGLPGTRRVVGAWVWNRNKEKVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDVRGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPYAHDISTLPPWDESRVENPDERVIIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(66397..67134)
                     /gene="trmN"
                     /locus_tag="N7B39_000060"
     CDS             complement(66397..67134)
                     /gene="trmN"
                     /locus_tag="N7B39_000060"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HGU2420131.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKAGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAATQAQENVAQSPWLERINVHT
                     ADIQQWVTQQTARFDLIISNPPYYEQGVECATPQREQARYTTSLDHQTLLTCAAECIT
                     EEGFFCVVLPEQIGNSFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            67266..68600
                     /gene="srmB"
                     /locus_tag="N7B39_000061"
     CDS             67266..68600
                     /gene="srmB"
                     /locus_tag="N7B39_000061"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HGU2420132.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(68633..69514)
                     /gene="yfiE"
                     /locus_tag="N7B39_000062"
     CDS             complement(68633..69514)
                     /gene="yfiE"
                     /locus_tag="N7B39_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU2420133.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEHSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEDCFLPG"
     gene            69617..70204
                     /gene="eamB"
                     /locus_tag="N7B39_000063"
     CDS             69617..70204
                     /gene="eamB"
                     /locus_tag="N7B39_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HGU2420134.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATTHGFHQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQT
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWIVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLIYCAVRIFY"
     gene            complement(70260..70643)
                     /gene="grcA"
                     /locus_tag="N7B39_000064"
     CDS             complement(70260..70643)
                     /gene="grcA"
                     /locus_tag="N7B39_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HGU2420135.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            70948..71637
                     /gene="ung"
                     /locus_tag="N7B39_000065"
     CDS             70948..71637
                     /gene="ung"
                     /locus_tag="N7B39_000065"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HGU2420136.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIATPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQHGETPI
                     DWMPVLPAESE"
     gene            complement(71685..72722)
                     /gene="yfiF"
                     /locus_tag="N7B39_000066"
     CDS             complement(71685..72722)
                     /gene="yfiF"
                     /locus_tag="N7B39_000066"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HGU2420137.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            72929..73348
                     /gene="trxC"
                     /locus_tag="N7B39_000067"
     CDS             72929..73348
                     /gene="trxC"
                     /locus_tag="N7B39_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HGU2420138.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            73417..74115
                     /gene="tapT"
                     /locus_tag="N7B39_000068"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             73417..74115
                     /gene="tapT"
                     /locus_tag="N7B39_000068"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HGU2420139.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            74147..76807
                     /gene="pat"
                     /locus_tag="N7B39_000069"
     CDS             74147..76807
                     /gene="pat"
                     /locus_tag="N7B39_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HGU2420140.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPGHV
                     AISNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATEIAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAKAHLLLQQAIAEGATSLDTHEVQPILQSYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMTWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDIAPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            76921..78276
                     /gene="pssA"
                     /locus_tag="N7B39_000070"
     CDS             76921..78276
                     /gene="pssA"
                     /locus_tag="N7B39_000070"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HGU2420141.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLLDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            78322..78645
                     /gene="yfiM"
                     /locus_tag="N7B39_000071"
     CDS             78322..78645
                     /gene="yfiM"
                     /locus_tag="N7B39_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HGU2420142.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKEFWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(78642..79940)
                     /gene="kgtP"
                     /locus_tag="N7B39_000072"
     CDS             complement(78642..79940)
                     /gene="kgtP"
                     /locus_tag="N7B39_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HGU2420143.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDSALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(80057..>80249)
                     /locus_tag="N7B39_000073"
     CDS             complement(80057..>80249)
                     /locus_tag="N7B39_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2420144.1"
                     /translation="KAIRQDGLFALVKKMPDATLRVLYSHICQIQQRIRFPQLAYFHT
                     CPPYQKFILKLLNNHFPAN"
BASE COUNT        19083 a        21872 c        20214 g        19080 t
ORIGIN      
        1 actgacgcga tttgccctac gtcatccatc accagcaatt gataatcacc tttatccgtc
       61 aaatgcaggg tcacgttgcg cccgcgttca gttaacggtt cgccattcag aaaccaccag
      121 cgttcacctg cccctccact tgattgcaac ggcaaagtcg cttccgctgc gcccggtaaa
      181 cgtttaataa tctcgccatc gcgaacgcct gtcagctgca gcgggagctg ggcatcgtga
      241 ccgtatggcg ggcaaattgt cgaggccggt ggtaagcgca cagcgcgacg ttctgatgcg
      301 ggcaaccagg gttccagcgg tagcggccag acatttatca tttcttgtcg cgcttgcggg
      361 caatcagcgg caacacgttt gccattttca tccagccaga tggggaaacg aatgccatta
      421 atgccttcct gctccggcag taatagagtt ggcggctgac tcccgtccag cagccaggtt
      481 gccaggcggc ggcgacagtt accgtcacct tccggcaaag actgtccgcc cggccagcaa
      541 ataacgccac gagtgactga gttcgggcgc gggtcttccg gcagattagc actgcgcgac
      601 agtaagatat tattgacctg attcaacaat ggtacggcgc tggcaaaacc aaactgacca
      661 acaacgggcg tgccgtccgg tctgccagtc cagatcccaa tgacatagcg agcgttaacc
      721 ccaatcgccc aggcgtcacg atagccatag ctggtgcccg ttttccatgc cagtggggcg
      781 acgcgcggca aggcaccatc cggcaagggt agcgcttcat cagccataat ccggcgaatg
      841 atccacgccg ctcccgacga cattaaaggc cgttcaagca gcggatcgtc aggctgtaag
      901 cgcaatttgc ctgccttgcc gtggcgagca aacgcggtat acgctgccgc catatcttcc
      961 agtttcgcac cagcgccgcc gagaatgagc gaaagattcg gcgcagcacc gttgggcaaa
     1021 tataacggca atccaacatt gcgtaacttt gccgcaaacc gtttcggtcc ataggcttcc
     1081 agcacctgca cagcaggtaa attcagcgag cgcaccagcg cctcgctcat gctgatcggg
     1141 ccatgaaaac cgctgtcaaa gttgccaggt cggtaatcac cggtacgccg ggggacgtct
     1201 tgcagcagtg atgccgggtg gatcaatcct tcatccagcg ccagaccgta aacaaacggt
     1261 ttgagcaccg atcctggcga tcggatcgca ttgaccatat caacatgacc aaagcgtgaa
     1321 tcatcgttga gatcaaccga acccacccag ccgcgaacac gcatatcggt atgatcaacc
     1381 acgatcatcg ccagtgagct gcgcggtggt aatcgtcctt tccagttttg cgccagttct
     1441 tccagacgtc gttgaagacc ggcatccagc gtagtaacga ttttgtcgct tttgctttta
     1501 ccgagcatca tgcgcgaaaa cagcggtgcc agttgcggca tttgtcgggg tgccagccag
     1561 atgggttctt cccttgactc ttttacctgc tcacgggacc acacaccttg cacggccatc
     1621 cgttcgagca ctttattacg cgcgacttcg gcacgctccg gccaacgatc cgggcgaaga
     1681 cggctgggcg cttgcggcaa aaccgccagc attgccgcct cggaatagct taaattcgca
     1741 ggcgactttc cgagataagc ccaacttgcc gcaccgatcc cctgcaacgt accgccaaac
     1801 ggagcgcggt taagatacaa ggtcagaatt tcacgcttag acagatgcca ttccagttgc
     1861 aacgcgcgcc agagctggcg aattttgccg ccaaatgttt tggggtgagg atcaagcaga
     1921 cgagcaacct gcatagtgag cgtgctgcca ccggaaataa cccgtcccga agtgagatct
     1981 tgccatgctg cgcgcgccac cgagaatgga ttcaccccag gatgcttcca gaaccagcga
     2041 tcttcataat tgatcagcgc ttcaaggtaa cgtggagaaa catcttcgat tgttacagga
     2101 taacgccaga tgccgtcagc atcggcgaag cgccagagcg gcgtaccatc ctgcgccacg
     2161 accactcgtg cgggattgac ttcatgcagc ggtagaggcc agagtttatc tgctccccac
     2221 gctgcaagaa aaaggagaaa tggcgcagct gccagcgtta tccagcagcc gcgtttggtt
     2281 aacaagcgag gcatttacgg tctgacaatc agcaaatctt cagccgcgcc ggtcgcccgc
     2341 cattggggaa catacattga ttccaccatc ggttgcggta cctgatacgt tcccggcgtc
     2401 accgcccgcg ccagatacac cagcgttacc ggttggtatt catcaacggc aaccgccgcc
     2461 acaaagcgat cgtcacggaa ctcaatgtgc ttaatactcg cctgctgcat ctggttcagt
     2521 aagttctgca cttcgccacc actttgctcc aggctggcgc taccgttcgc cagattctgg
     2581 ttttccagtt ccagacccgc aggcagcaga tccacgacta acgcatccgg cacgctgtta
     2641 ctagctttta cctgcaacca gaccagcacc agatcgccgc tacgtaacga gtccagcgat
     2701 ttgctcttac catcagtacc aagaatatga cgctcaattt gcagcacatt gttcgccggt
     2761 aaaggtgcgg attgcggata accgctggca tccacacgca gccataacgg ctgctcgcca
     2821 ctgttggtca cctgcaaggt ggcaagttga tcgctattca gattgctgtt ttgcgctttc
     2881 tcgcctgtca gcggctcagc tgagaaagag gtttgcgcct gccatttacc tggtaaatcc
     2941 tgaatcgtac gggcagccag gaacaacgcg ttactttcct gcgtcgatag ccagcgttca
     3001 ccaaacgcct gctgcgaaag tgtgttcagc agggagtact gctcatcggg tagcagtttg
     3061 ttctcttcca gcaaggagag catcaacgcg ttgtcgcgca gtggactacc gtaatcaccc
     3121 agccatatcc gctcatcact attacgcggc gtttttagcg ccagcgcaat cgcttcttca
     3181 tcacgcgtcg catcacccat ggttttcagc gcaacgccaa gttgcagcag cggtaaacca
     3241 gaagcggcat ctgcacgatg ctcccagatt tcacgcagcg cacccagcgg agccttttgc
     3301 tgacgggcca acaccagcgc agcgtaagac tgtacggcga atttactggc tttgagatta
     3361 tccgcgtacg ggatcgacat catgcccgga tcttgtaaat agcgcagcag acgctcatta
     3421 ccccggttaa tggcgtctgt cggcacgctg taaccctgtt cgcctgcgcg gaccaggaaa
     3481 tccatcacgt aagccgtcag ccagtactct tcgtcaccgt ttttatccca cagcgcaaag
     3541 ccgccgttat cacgttgcat ttgcagcaaa cgggaaatgc cgatatcgac cgatgcacgg
     3601 cgtttctcat cactgtcgcc tttgatgccc aacgcctgca gttgggctgc gttggtataa
     3661 agtgacggga acaggccgct ggcggtttgc tcaagacagc cgtacggata cgcttttaac
     3721 tctttgatat aacgtgcgat gttcagcggt ggtttgccgc tcaacaacaa ttgcccttcc
     3781 agcgtaacag gcgagaagtt ttgcaatcca tccgccggaa tcgcccatgt ctcaccaggc
     3841 tgtaacgccg taccgtaatt aaccgtttgt gccgggaacg ccggacggac gccgattttc
     3901 cactgcttat gctgatcggc aacggtttca cccggtaacg ctaacccgct aatggtggcc
     3961 tgaatttctc catcgccata acccggcaat gctcgcaccg ggataaacag cgtagtacgc
     4021 acacctggcg ctaattcaac ggctgcgggt gaatcgctga ccagttcaag caaaccactg
     4081 gcggtcaggg caacgttcag tttttgcggt ttatcggtaa gattagtgat atccagcgtc
     4141 agacgcgagt tatcgccact cgccataaag cgcggcatgt tcagttcagc aatcaccggt
     4201 gcggcaacta tcactttgct ttcgttgcta ccgaattcat ctgccgtcca ggcttgcgcc
     4261 atgacgcgca attcaccgtt aaaatcgcca atcggcagtg taaccgagcc ttcgccctgt
     4321 tcgttgagcg ttaccggcag cgcctgctgc gcgacaatat tgacgtgatt gaccggcggt
     4381 ttaccaccac gtttcagctc atcaccatcg ccaccgaaac gcagagctgc cagacgcccc
     4441 tgaccttcaa taacctgacc gtaaatatcg taaatgtctg cgccatagcg tttctgacca
     4501 aagaacgcct gccacggatc tggcgtgacg tagtcagtaa tattcagcac accactatca
     4561 acggcagaca ccagcacatt cacctgttta ggcttctcgc catttttagt gctggcttta
     4621 attttcacgg ttaatggttg attgggacgc atttttgctg gtgtttccag cgccagatcg
     4681 aggcgacggt tttcatcacc aagcggcaga tgcaacacac caaccgcgcg ttttggcgtc
     4741 gcggagcgag atttatcgcc aggacgcacc accagcgtac tcaaataaag atcatgacga
     4801 ttccaggttt tatcgaccgg aatcgtcaga tccagccctt gagccggaac atcaatctct
     4861 tgccaccaca gcggcccttc actggactcg accatcgcat aacctttacc cgccgttggc
     4921 gcggcgatat gcaacttaat ggtatcgcca gggcgataac cggctttatc cagtttcagc
     4981 gtgacacggt cgggtcgcac tgcgccgcta ccgtcgctat tatcctgcca gctatagcca
     5041 gcccaaaaac gaacactact gaccgcttca ttcggcgctt tgacttccag acgataagcg
     5101 ccccactcta ccgggaaact gaccttgccg gtttcgtccg ctttcagatc cagagtttgt
     5161 tcattttcaa tcagatcttt ttgatcaaac tgtgattgcc agccttcatc ttctgaccag
     5221 ttccagtagt aatcgcggcg ttcgcgaatc aggcgcacct gcaagcccga cacggctttt
     5281 ttcacgcctt gcgcatcgct ataaacgatg tcaaaagcgg cgttactgcc ttcatcaaca
     5341 atgggctgtt tcaccgtgct gtcagtgcga taatcgtaaa ccgatttcga ggcgaactgc
     5401 ggacggatcc ccggcaatgc atcggcaggc cagatagcct gctcagcgcg gcgcgtcacc
     5461 gggcgaccgc ccgattccag caaactgccc tggaaaataa cctgcaatgg ggaatgcgtt
     5521 tccttccact ggctttctgt agaaacttcg ccgcgccctt tatcatccag cgtcaactga
     5581 acttcatcca gcgtgcggga aaggttttcg gcagctatat cgccgaattc aaaaccaggt
     5641 aaggctgaca cagcctcacg cagtgggcgc aggaaaagtt gcccttgcaa agtattacca
     5701 ttagcaggcg caccgtacag gtagtatccc accacggaga atttcacttc atcattcggc
     5761 gtaagcgggg ttttctcacc ggtcagattc agcgccatac gctctggcat aaaatcttcg
     5821 acgtggaaat cccacatccg atactggtta tcgcccgtgt tagcgcgaat atgccacata
     5881 ccggttgccg cattgctatc gagtggccag gtaaagtggt agaggccatt ttccggctga
     5941 ctaacgacgc tcctgagtac ctgcccatca ggtttaatca cgtctaactt gatgggttga
     6001 ttgggcaacg ctttaccgtc tgcatcacgc agcaaaccat tgaggattac cgtttcaccc
     6061 gggcgataaa gatcgcgcgg gccaaacatg aaaaactgtt tgctatagcc tggcgcgcca
     6121 gcaatgttaa attctgctaa gtccagcgcc ggaagtttta aatcgagtag cgttgtctga
     6181 ccgtctttac gtgccagcaa taatgcggcg tttttatcat tttccagctg cacatgcccc
     6241 tgagcgtcac tggttgcctg agtcagagtc tgccctttct catttaataa agagacttca
     6301 attccttgct gggccgcgcc gttttccaga ctttgggtaa agatatccag acgattgtga
     6361 taacggtgag ctgaaacgcc gatatcactt aacgtaaaca gcgtcgcggg attactgtaa
     6421 tcgtaacgtc cagcctgatt catcacagcc agatacacgc ccgcctgctg aagcggtttg
     6481 atatcgccca gcggcagcaa taatttttca cgggtgttac gcgcaggatt gagatcaaac
     6541 cgtccggtgt agaccagatc cgccatctgc agcagtttgt ctgactgcca gttcgccagc
     6601 gaattgcggt attcccattg gctaatgaat gctggcagag attctggctt aacgcggaag
     6661 aagttaacat cgacattatt aacgttgagc gccattaccg gcagcccttc aacgacttta
     6721 ccaggcagca gcgaaccacg gctggcaaaa ccgacactgg gttggatgtc gcgggtagtt
     6781 atagtttttt cgtaatcttt actgaaggtt gcgttgttga gtgctttgac ctccttgcca
     6841 attgtaacga tcaaatcacg tttcggttcg aggtggcgta aacgcagctc tttaagatta
     6901 tctgatagct cccaggcacc atccactttg ccgctttttt tatcgacgac atgaataacg
     6961 cgtgagaaat cctgatccgg gtcgagaggg atggagaacg tcagcaccag cgtggctgca
     7021 ccatcaagtt ggacttcaga gagatccagc aatgtcagcg ccttaccggc actctgttgg
     7081 gccagttttt gtcgctctgg cgcggagagt tttgctgaac tcgcgttttc tgaagaggcc
     7141 gctttagtaa cttcagaagg cgcatctttt ttcaccgctg ttggcgcgtt atcgttgttg
     7201 tcgcaccctg ccagcgccag cattagcatg caggcggcta cgcgtaactt tttcattttt
     7261 catccctgcc acaatggccc gttagcaacg tcgaataatt attatgcgtg agaatttacg
     7321 ttatgtaatt cagtatcacg cccatacaat ccgaatttta gtgagaattg tggctcagac
     7381 agccaaatca gcctcttttg cttgtcgctt ttcactaaac atccgacaat ttagcctaac
     7441 cccggcaaaa atggagatgc ctatgtccac gacatggttt gtaggagccg actggctcgc
     7501 cgaacatatt gatgacccgg aaattcagat tatcgatgcc cgcatggcgt cgcctggaca
     7561 ggaagatcgt aacgttgctc aggagtatct gaatggacat attcccggcg cagtgttttt
     7621 tgatatcgaa gcgctttctg atcacacttc cccgcttccg cacatgctgc cgcgcccgga
     7681 aacgttcgcc gtggcgatgc gtgaattagg cgttaaccag gataagcacc tgattgtcta
     7741 tgacgaaggt aatcttttct cagccccacg agcatggtgg atgctgcgca cctttggtgt
     7801 agagaaagtg tcgattctgg ggggtggact tgcaggctgg cagcgcgatg atctgttgtt
     7861 agaagaaggt gcagtagagc tgccggaagg agagtttaac gccgcgttta atcctgaagc
     7921 agtggtgaaa gtaaccgatg tgttattggc aagccatgaa aatacggcgc aaattattga
     7981 tgcccgcccg gctgcacgtt ttaacgcaga agttgatgaa cctcgcccag gtttacgtcg
     8041 cggacatatt cccggtgcat tgaacgttcc gtggacggaa ctggtgcgcg aaggcgaact
     8101 aaaaacgacc gatgaactgg atgcgatatt ttttggtcgc ggcgtcagct atgacaaacc
     8161 aattatcgtc agctgcggct ctggtgtaac agcagccgtg gttttgttgg cactcgcgac
     8221 gctggatgtg ccaaacgtga aactgtacga cggcgcatgg agtgaatggg gcgcgcgggc
     8281 agatttaccg gttgagccat tgaaataagc tttttacagg caataaaaaa ccgccgaatt
     8341 tggcggtttt ttattgctgg tctggttcgc ggcctttcca gcaggttgac ttgtgttgcc
     8401 ggagcaacgc aggcacccca cagtgttgaa atactctctt ttatgcctgg ctgtgtcaag
     8461 ataataaaca gagtaattat tcaaacatga gagtattatt cttaatggcc cttttcgccg
     8521 tctcgcaaac gggcgctggc tttaggaaag aatgttccat gaccgtaaat acaggcaccc
     8581 cacagtgctt gctatcgcgg caatatcgcc agtggtgctg tcgtgatgcg gtcttcgcat
     8641 ggaccgcaca atgaagatac ggtgcttctg tatcgtactt attgtttctg gtgcgctgtt
     8701 aaccgaggta aataataacc ggagtctctc cggcgacaat ttactggtga ttaacaacct
     8761 tcagagcagc aggtaagccc gaatgccgcc ctttggggcg gcatatttta gattatccga
     8821 ttctgtttaa agtcacgcaa aaaaccaccc cagcgacgtt catagaatgg cgcaatatgt
     8881 tcggtaataa agtggctaat tcctttttcc ccttttttca cctgacaaat atcgattggt
     8941 tcatcgccag gtaatgtatc ggtcgctaca cttcccgcca cctgaataat ttcttcgata
     9001 tcaccatcgg cttcaatgcc aataagtaaa ttaggctgtg cctcttcgtt ctctttaatt
     9061 gaacaaataa aagcacgctt cacaggctta atggttttaa ataaggtggt gagtgaatca
     9121 atcatttgtg ctggcggctc tgcgacttcc gataatatca gcgattcacc gccttccagg
     9181 atttcctggc tgctcagcgg atttccctct tcaccaatca acaaactgat ttcacgcggc
     9241 ataaattctt taccggtcgg cagtttggca ttaaggaaga gcgtttcgcc aagggtcatc
     9301 tcaaacaatg tgcgaacggg cattacgaca aatgcctgtt cgtcttcaac cgcctgttga
     9361 agtgcttcta acgaggtaaa aaaaggaatg acgctggtgc cgtcttcttt ttcccagtgc
     9421 tgtaaatcaa gcgcgctatc ttcaaccaca gcctcgccct gcgccgccgt accaggcacc
     9481 cagacggtgg attccagtag agtacggaaa aaggccgggc ggtgcgccgg ttcggttgct
     9541 gctttttcca gcaggtcttc aagttcattt tttgtttcgg acataagaac cacaattcat
     9601 tcaacgttcg gggcacaagt gcctgatgcg ccatacttat caggcctaca agggattcac
     9661 aatttgttga atttgcaata ctttgtaggc cggataaggc gtttacgccg catccggcat
     9721 attagtttac gccgttaaca gattagcaat cgtgcgcaca ccaagtcccg tagcacccgc
     9781 agaccactgt tcaaccggcg ctttacggta agtcgccgag cagtcgatat gcagccagcc
     9841 ttgctgatag ttctcaacaa agtgcgacag gaagcccgct gccgtgctcg cgcctgccgg
     9901 atacgccgcg cttccggtat tgttcagttc ggcaaagtta gacggcagct ggctgcggtg
     9961 gaattccgcc agcggcagac gccagaatgg ttcgttctct tgtgaggcac tcgccagcaa
    10021 acgaccggca agcgcatcgt caaaactgaa cagcgcgtga taatcattac ccagcgcagt
    10081 tttcgccgcc ccggtgaggg tcgccgcatc aatgatcatt tccggtttct gcgcactggc
    10141 atcaatcaga ccatcggcaa gcaccaggcg cccttcggca tcagtgttca tcacttcaac
    10201 ttttttaccg ttgcgataag tgatgatatc gcccagcttg aacgcattgc cgctaatcag
    10261 gttatccgca cagcagagga acagcttcac gcgcttgttc agtccgcgcg taatggcaaa
    10321 tgccagcgcc ccggtaaccg ttgccgcgcc gcccatgtcc gacttcatcg agtccataaa
    10381 cgcagtctgt ttgatgctgt agccgccgga gtcaaaagtg atacctttac ctaccaggca
    10441 cgcgtacact ggcgcttctt tatcgccagt tgggttgtaa tccagcgcca gcaataccgg
    10501 agaacgttct gaaccgcgtc cgacggtgtg cagccccata taaccttgct cacgcagatc
    10561 ttcgcctttg gtgatccgat aagtcacacg atcgctcgcg acgttgctga tcagatcaac
    10621 agcacgctgt gccagttgcg atggtcccaa ttcttctgcc ggtgcgttga tggtgtcacg
    10681 cacccagtcg atgatcatca ggcggttatc cagttcctgg cgctgggcat cgtccagatc
    10741 cggccactcc actttacgcg tgcctttcgg ggctttgtaa ccttgccaga atgcccagca
    10801 tcgatccgca tcccagcctt caccgcttaa ctgaacatgc ttgatgccca gaccgtcaat
    10861 cttgcgcgcc gcacgctgga tcagccccag atcgtctgcc ccgttcaaat gcagggtaat
    10921 gccgtcatta ttaatgctgt aagttgcttt ttctccccag cgcgcgtcgg caggttgggt
    10981 agagagggta atcttcatcg cttctgtcat tttagttatc cttctttgta aaagggccgc
    11041 cagttggcag ccccaggatt tttccgctaa atgattcgcg ttgcaggaag gcggcaagtg
    11101 agtgaagccc caggagcata gataactatg tgactggggt gaacgagcgc agccaacgca
    11161 tctgcggcgt gaagcatgac gcggaaatta ttcggcctcg tccagccaga ctaacaaaat
    11221 cgcttcgagg attttctcgt tggatgcctg cgggtcgtcg tcgaaatctt ccagatcgca
    11281 aatccactga tgcatatcgg tgaatcgaac cgttttcgga tcaagatcgg gatacgcatc
    11341 gtacagtgct tcgccaattt cgcggctatc ggtccactta agtcccatac taacctctgt
    11401 taatgctcac gcgcatggtt gatcgtgtaa cgcgggattt cgaccactaa atcttcgtcg
    11461 gtgacgcgcg cctggcagct taaacggctt tccggctcca gtccccaggc tttgtccagc
    11521 atgtcgtctt cctgctctga gctttctgga agcgagtcaa aaccttcacg aacgatgcag
    11581 tggcaggtgg tgcaagcaca ggatttttca caggcgtgtt caatctcgat accgttacgc
    11641 agagctgcgt cgagaatggt ttcaccgcta ttagcttcca gaacagcgcc atcagggcag
    11701 agatcctgat gaggcaaaat aacaatcttt ggcatattaa acctcgtcca cggaatggcc
    11761 tttcagcgca cgacgaaccg actggtccat gcggcgagcg gcgaaatcct gggtttgttt
    11821 gtctacgttt ttaatcgctt gttcgatggc gtcaacgtca tcgccctgcg ccacttcact
    11881 caggtgagcg gcagcatcgt caatgacctg acgttctgcg gcgcttaaca gcgcggcatc
    11941 agcagccagc gcgccgtgca gactttccag cacacgcgcc gcttctactt tttgttctgc
    12001 cagcattcgg gcttttacgt cctgctcggc atagctcatt gagtctttga tcatcgaagc
    12061 gatttcgcta tcggtcagac cgtaagacgg tttgacctga atagacgcct caacgccggt
    12121 ggatttctcc atcgccgtca cgctcaaaag accgtcggca tcgacctgga acgtcacgcg
    12181 aatatgcgca ccgccagccg gtagcgccgg aataccacgc agcgcaaaac gcgccagtga
    12241 gcggcagtcc tgcaccagtt cgcgctcacc ctgcattaca tggatagaca tcgccgtctg
    12301 accatcttta aaggtggtga aatcctgagc gcgggccact ggaatagtgg tattacgcgg
    12361 aatcactttc tccaccaggc cgcccatcgt ttcgaggccc agcgacagtg ggatcacatc
    12421 aagcaacagc atttcgatgt ctggcttgtt acccaccaga atatccgcct gaatcgccgc
    12481 gccaatagcg acgactttat ccgggtcgat ggaagtcagc ggtggacgac cgaaaaattc
    12541 gcctacccgt tcacgcacca gcggcacgcg agtagaaccg cccaccatca ccacttccag
    12601 cacttcatca gcttctacac ccgcgtcttt cagcgcgcga cgacaagcca ataaggttcg
    12661 ttttaccagt ggcgcgatca gttcattgaa ttgttcacgg ctgatttcgc cctgccagcc
    12721 cgcaacgtta acggtcacgg agtccgcatc gctcagcgcg attttggctg caatggcggc
    12781 atccagcagt tcacgctgaa cgcggttatc gctacgatca ggaatgcccg cctgctcgcg
    12841 aatgtaatcc gccagcagat ggtcgaaatc atcgccgccg agcgcggaat caccgccggt
    12901 tgccagcact tcaaacacgc cgcgacttaa gcgcagaatg gaaatatcaa acgtcccgcc
    12961 accgaggtca taaacggcga tcacgccttc ctgaccggaa tccagcccgt aggcgatagc
    13021 cgcagcggtc ggttcgttaa gtaagcgcag gacgtgaagg cccgccagac gcgccgcgtc
    13081 tttggtgccc tgacgctggg catcgtcaaa gtacgccgga acggtgataa ctacaccatc
    13141 cagctcgcct gccagggctt cagttgcccg cgccgccagt gctttgagga tgtccgcaga
    13201 aacgcgcacc gggttcagca gccccgccgc cgtttcaatc atcggcaggc cgttttcgct
    13261 ggcctggaat tgataaggca gatgcggata gcgttgctgg atatcagcca gcgagcgtcc
    13321 catcaggcgt ttaacagaac taattgtgtt ggcggtatcg agcgctgcat tagtacgcgc
    13381 gtcataaccc accgaatgcc cttgctgttg atagtgaaca acagatggca gcaggtgacg
    13441 gccttcatgg tcggctaacg tttcggcctg accgctgcgc actgtcgcca ccagcgagtt
    13501 ggttgtgccc aggtcaatac cggccgccag acgacgctga tgcggcgcag cactcaaacc
    13561 aggttcacta atttgtaata aggccatgtt tagcttccag aaattaaaaa tcgagcagtt
    13621 tttcttcgag ttgttcggca ctgcttcgca gtttatcgag aaaacgcagc ttacgcacgg
    13681 tatccgccgc cgcgtcccag gtctcgttgt ctaactgttc aaccatcaac tgatggcggg
    13741 tatcaaacat ctttttcaca cgtttgataa agctttccag ccgcgcttca tctttcgcct
    13801 gttcgatctc gtccagctct tcgcgcagct ccaactgttc catcaggaac gcggtgtcgc
    13861 gcacagtatg ctgctcgctg gcgagatcaa agccgtgcaa agaaagcaaa tattccgcgc
    13921 gcattaacgg atgacgcagc gtttgccagg cctgattaat ggttgcagat tgctgtacgg
    13981 cggcgagttg ttccgcctgg cttccgctgg cgaatttatc aggatgatac tgacgttgta
    14041 gatcctgaaa acgcaggctc agcgcctggg tatcgagttg ataacgggca ggcaagccaa
    14101 agagggtgaa gtaatccata acattctcag ggctgcgatc cgccgcgata acggcgaatc
    14161 aggtaaaaca acccccacgc gcaggcgacc acggtggggt tatcggtatg cgcatcaaac
    14221 gtggaagctt tcgccgcaac cacactcatc tttaacgttc gggttggtga atttaaaccc
    14281 ttcgttcagg ccttctttta cgaagtccag ctgcgtaccg tccagaaatt gcaggctttt
    14341 gccatcgacc acgactttca cgcctttgtc ttcaaacacg atgtcttccg gcgtcggttc
    14401 gtcaacaaat tccagtacat aagccatacc tgaacatccg gaggttctca cgcccagacg
    14461 taggccaaac cctttgccac ggttagccag aaaggtattt actcgcgctg ctgcactgtc
    14521 gctaagtgta atcgacataa ccaaacctca actcttattt tgcttcacgt ttgcttttat
    14581 agtccgcaat ggcggctttg atcgcgtctt ctgccagaat agaacagtga attttcaccg
    14641 gcggcagttc aagttcttca gcaatatcgg tgtttttgat agcctgcgct tcgtcgagag
    14701 acttcccttt cacccattcg gtgaccaggg agctggaagc gatagcagaa ccgcagccgt
    14761 aagttttaaa acgcgcgtct tcaatgatac cttcatcgtt gactttaatc tgcaacttca
    14821 ttacgtcgcc acaggccggt gcacccacca tgccgctgcc gacgttctcg tcgttgttgt
    14881 caaaggaacc cacgttacgc ggattctcgt aatggtcgat aactttttcg ctgtaagcca
    14941 ttataaattc tcctgattcc gataccgatt aatgatgagc ccattcgatg ctgttcagat
    15001 ccacgccctg cttgtacatt tcccacagcg gagaaaggtc acgcagacga ccgatggatt
    15061 tacgaactaa ctcgatggtg tagtcgatct cttcttcagt agtaaaacga cctaaagaga
    15121 aacggataga gctatgtgcc agctcgtcgt tcagccccag cgcgcgcagc acgtaggacg
    15181 gttcgaggct tgctgacgta caggcggaac cggaagaaac cgcgagatct ttcagcgcca
    15241 taatcagcga ctcaccttca acgtagttga agctgacgtt gagaatgttc ggcgcaccgt
    15301 gttccaggtc accgttcagg taaacttctt cgatatcttt gatgccgttc cacagacggt
    15361 tacgcaggcc gcgcagacgt tccatctcgg tcgccatctc ttcttttgcg atgcgatagg
    15421 cttcgcccat tccgacgatc tggtgaacag gcagagtgcc ggaacgcata ccgcgctcgt
    15481 gaccgccgcc gtgcatttgc gcttcgatgc gtacgcgcgg tttacgacgc acatacagcg
    15541 caccgatacc tttcgggcca tagattttgt gaccggagaa agacatcagg tcaactttca
    15601 actggctcag gtcgataggc agtttgccca cgctctgggt tgcatcaacg tgatagataa
    15661 tgccacgagc acggcacatt tcgccgatag ccgcgatatc ctgcaccacg ccgatttcgt
    15721 tatttacgtg catgatagaa acaaggatgg tgtcgtcacg catcgctgct tcgagttctt
    15781 tcaggtcgat aataccgtta cgctgcggtg cgaggtaggt gacttcgaaa ccttcgcgct
    15841 ccagctgacg gcaggtatcc aataccgctt tgtgttcggt tttgctggtg attatgtgct
    15901 tgcctttttt ctgataaaag ttggctgcac ctttgatcgc caggttgtca gactcggttg
    15961 caccagaggt aaagacgatt tcacgcggat cagcgccgac cagatcggca atctgattac
    16021 gggcgatatc taccgcttct tcagcctgcc agccgaaacg gtgagaacgg gaggccgggt
    16081 taccaaaggt tccgtccatc gtcataaact gcatcatttt ctcggcaaca cgcgggtcca
    16141 ccggcgtggt tgcggagtag tcgagataaa tcggtaattt cattgctcta taaactccgt
    16201 acatcactca atgcaaggaa ccaggctacc ggctggatgt acgaccgtgt ttacggagcg
    16261 cgatacgccc cggcctgatt ctgaatactt tttattaagc gcgtaactta acgtcgatcg
    16321 cgtcttgtgt gcgggtgcgt ggcgcgtcgt gagtatgctg acgaccagac acatccagca
    16381 cttcctggtt gttaaccagt tcgcctaaag taatgttatt gagaaaaccg gtaagacggt
    16441 cgctcaaatc acgccacagc gcgtgggtca ggcatttatc gccgccctgg cagccgcctt
    16501 taccctgaca acgggtggca tctacagatt cgtcaacggc gctaatcact tcgccaacgg
    16561 cgatgctgct ggcatctttg cctaacagat aaccaccgcc tggtccacgt acgctggaaa
    16621 ccagaccatt tttacgcaga cgagaaaaca gttgttccag ataagaaagg gaaattccct
    16681 gacgttcgga aatatcagcc aacggtaccg ggcccgcttc agagttgagc gcaacgtcaa
    16741 gcattgcggt cacggcatag cgccctttag atgtcagtct catgtcttac ttcacctcaa
    16801 actcgcccct gcccggggtt ttttattgta aaagtggggg tattgcatag cagggtcaag
    16861 tctgacattc ccgagtaaat tggtcaacta tttacttgac tgatttagtc gggtatttaa
    16921 ccttcagtgc cattttttta tcgtggcgtt tgtacctgtt atccttccag accatgggcg
    16981 ggttgagggc aattattcgc attagcgcag gcctgttccc ctcaccctaa ccctctcccc
    17041 gaaggggcga ggggaccgtc cgtgcactta tctggcttag ccctcaggcc ttattacctt
    17101 tattctgctg ctctatagaa gccagaatcc cgcgcaggat attcaattcc tggctttccg
    17161 ggcgcgcacg ggtaaacaga cggcgcaatt tattcatcac ctgccccgga tggttttcac
    17221 ggataaaacc ggttgccagc agcgtttgtt ccagatgacc ataaaaacgc tccagatcat
    17281 cgaccagcgg atacggcgtc tcttcatgct cgacctgctc gccattttcc tgagttgcca
    17341 gccaggccat acgcacttca taggcaataa cctgaaccgc catcgccagg ttcagcgagc
    17401 tgtattcagg gttagcggca atcgcgacat gataatgaca cttctgcaac tcttcattgg
    17461 tcaggccgac acgttcgcga ccaaacacca gcgccaccgg ggtatttgcc gcttcagcaa
    17521 cgcttttcag accgcattcg cgcgggtcga gcatcggcca cggcagcgtg cgggaacgtg
    17581 cgctggtgcc caccaccagg ctacaaccgg ctaacgcttc gtcgagcgta tcgacgatgt
    17641 gagcattacc aatcacatcg ctggcccccg ccgccagggc aatcgcctgg gagtcaggtt
    17701 tcaccagtgg attaaccagc cacagattgg ttaatcccat tgttttcatg gcacgggcaa
    17761 cagaacccat attgccggtg tgtgacgtct ccaccagcac aattcgaata ttttgcagca
    17821 ttgtctttct tcgtctaaag attattcgcg catcttatca taaaacgaag acagatgccg
    17881 atctcgctgc tatactctgc gccgttttcc cgttctttaa catccagtga gagagaccga
    17941 tgcatccgat gctgaacatc gccgtgcgcg cagcgcgcaa ggcgggtaat ttgattgcca
    18001 aaaactatga aaccccggac gctgtagaag cgagccagaa aggcagtaac gacttcgtga
    18061 ccaacgtaga taaagctgcc gaagcggtga ttatcgacac gattcgtaaa tcttacccac
    18121 agcacaccat catcaccgaa gaaagcggtg aacttgaagg tactgatcag gatgttcaat
    18181 gggttatcga tccactggat ggcactacca actttatcaa acgtctgccg cacttcgcgg
    18241 tatctatcgc cgttcgtatc aaaggccgca ccgaagttgc tgtggtatac gatcctatgc
    18301 gtaacgaact gttcaccgcc actcgcggtc agggcgcaca gctgaacggc taccgtctgc
    18361 gcggcagcac cgctcgcgat ctcgacggta ctattctggc gaccggcttc cccttcaaag
    18421 caaaacagta cgccactacc tacatcaaca tcgtcggcaa actgttcaac gaatgtgcag
    18481 acttccgtcg taccggttct gcggcgctgg atctggctta cgtcgctgcg ggtcgtgttg
    18541 acggtttctt tgaaatcggt ctgcgcccgt gggacttcgc tgcaggcgag ctgcttgttc
    18601 gtgaagcggg cggcatcgtc agcgacttca ccggtggtca taactatatg ctgaccggta
    18661 acatcgttgc tggtaacccg cgtgttgtta aagccatgct ggcgaacatg cgtgacgagt
    18721 taagcgacgc tctgaagcgt taatgactca ggcgggtgat atcactcacc cgccctcgcc
    18781 tttcaggcgc tattccgaaa tacttcctca ccgctttact ttctttcgtc actctcccac
    18841 catctttccc cgattaatgg atgataaaga agtcgcaagg aaaggttatg gcactgccag
    18901 tgaacaaacg cgttcccaaa attctgttta ttctctttgt tgttgccttc tgcgtttatt
    18961 tagtgccgcg cgttgccatc aacttcttct attatcctga cgataaaatt tacggtcccg
    19021 acccctggtc ggcggaatcc gtcgaattta cggctaagga cggtactcgt ctgcaaggct
    19081 ggtttattcc ttcttcgacg ggccctgctg acaacgccat cgcaaccatc attcatgctc
    19141 acggcaatgc cggaaatatg tccgcccact ggccgctggt cagttggtta cccgagcgta
    19201 atttcaacgt ttttatgttt gattatcgcg ggtttggtaa atcaaaaggc acgccgtccc
    19261 aggccggatt gctggacgat acgcaaagcg ccatcaatgt ggtgcgccat cgcagtgatg
    19321 taaacccaca acggctggtg ctgttcgggc agagtattgg cggggcgaat attcttgctg
    19381 ttattggtca gggtgatcgt gaaggcatac gtgcagtgat cctcgactcc acatttgcct
    19441 cttatgcaac catcgccaac cagatgatcc ccggcagtgg ctacttactt gatgagagtt
    19501 acagcggcga aaattatatc gccagcgtca gcccgatccc gcttttactc attcacggta
    19561 aagctgatca cgttatccca tggcagcaca gcgaaaagtt gtatagcctg gcaaaagagc
    19621 caaaacggtt gatcctaatc ccggatggcg aacacattga tgctttttcc gatcgtcacg
    19681 gcgacgttta tcgcgaacag atggtgaact ttatccttag cgcgttgaat ccgcagaact
    19741 aacccatgat cgctagcacg ataatcattc acaaaatcac cttaagacat gctaatccac
    19801 tggtcaggac agtttaagat gagaaaaatt ctgtgacgct tgccaacatt tctgatgatt
    19861 agcattccct tcgccatttc cctgagcaaa ctttagctat tcttatcaat tatgcttatg
    19921 ggagatctac agatgatgcc tacgcttgct ccaccatctg tcctttcggc tccccagcgc
    19981 cgctgccaga tcttgctgac gctctttcag ccggggttga ccgccaccat ggcaaccttc
    20041 agcgagctta atggtgtgga tgatgatatt gccagtcttg atatcagcga aacaggacgg
    20101 gagatcctgc gctatcatca actcacactg acgactggtt atgacggtag ctaccgggtt
    20161 gaaggtacag tgcttaacca acgtttgtgt ttatttcact ggctacgacg tggtttccgt
    20221 ctgtgtccgt catttattac cagccaattc acccccgccc tgaagagtga actgaagcgg
    20281 cgcggaattg cgcgtaactt ttacgacgat accaatctac aagcgttagt gaatctctgc
    20341 tcccgacggc tgcaaaaacg ctttgaatcg cgcgatattc atttcctgtg tctgtatctg
    20401 caatattgtt tgctgcagca ccacgctgga attacgcctc agtttaatcc gctccaacgt
    20461 cgctgggcgg aatcctgcct tgaatttcag gtagcgcagg aaattggacg ccactggcag
    20521 cgtcgggcgc tccagcctgt accacctgat gagccactgt ttatggcact acttttttcc
    20581 atgttgcggg ttcccgatcc attgcgggat gcgcatcagc gggacagaca attgcgtcag
    20641 tctatcaaac gtctggtaaa ccattttcgt gagctgggaa atgttcgttt ttatgatgaa
    20701 caggggttat gtgatcagct ttatacccac ctcgcccagg cgttaaatcg cagtttgttt
    20761 gccatcggta ttgataatac cctgccggaa gagttcgcca gactgtatcc acgcctggta
    20821 cgcaccaccc gcgcggcgct ggccggattt gaaagtgaat acggcgtcca tctttctgat
    20881 gaggaaagtg gtctggtcgc ggtgattttc ggtgcctggc taatgcagga aaacgatctg
    20941 catgaaaaac agattattct actgaccggg aatgatagcg agcgagaagc gcagattgag
    21001 caacagttac gcgaactaac gttactgccg ctcaacatta aacatatgtc ggtaaaggca
    21061 tttttgcaga caggcgctcc gcgtggcgcg gcactgatta ttgctcctta taccatgccg
    21121 ttaccgctct tttcaccacc gctgatctat acggacctga cactgacaac acatcaacag
    21181 gagcagatcc gcaaaatgct cgaatcagca tgagggaaca actttcgggc gcaaaaacat
    21241 tgccggaagc gccaccagcg ccatcaccca gaacacgccg tggcccagat attgatacag
    21301 gaaaccggcg aaaacggtca tgatagcgat actgccgccc atcgcgacgg cagagtaaac
    21361 cgcctgtaaa cggatgactt cgctaccctg gcgagcagca atatagcgca tggcggccag
    21421 gtggcagacc gtgaaggtgc cgcaatgcag aatttgcacc actatcaacc acggcaacgc
    21481 cgtagttgct cccataatgc cccagcgcac tacgccgcaa atcgctgaga tcaacagcat
    21541 atcgcgtgca ctacaacggc ggaaaagttt attactcagt gcaaagataa tgacttccgc
    21601 caccacgccc agcgaccaca aataccccac cgccgaggcc gagtagccag ctgcctgcca
    21661 gtaaatggcg ctaaaaccgt aataggccgc atgtgccccc tgcaataaac aaacgcaggc
    21721 cagaaagcgc cagttctggc gaaccagcgc caaccacgct gaccaaccgg tgctctcctg
    21781 ctggcggctt gccccttgtg gctgaatcgt cggacggatg agaaagccga gcagcatgga
    21841 tgccacgccc aacgtcaaca gcacgaggat cacccgataa tcaaacatag tgaccagttt
    21901 gcccgtcagc gccgagccaa tgacaaacgc caccgagccc cacagtcgca ctttgccgta
    21961 atcaagcggg aactgctttt gccacgtatt cgccagtgca tcggtcaacg gtaccagcgg
    22021 tgagaaaaag aggttaaagc caatcatcac cagcatcagc cacgctacgt gcgcccccgc
    22081 ccagaaggcg acagcaaaga gaagtgtcag cagtgccagc acgcgcaagg cggaaatcag
    22141 gcgagaagga tcgctgacgc ggggcgcgat gagcaaactc ccgagaaaac gggcaaccag
    22201 acctgccccc aataacaggc cgatggtttc tggcgttaaa ccaatccctt taagccagac
    22261 gctccagaaa ggtagaaaaa tgccgtaact aaaaaagtat gtgaaatagc cgagcgccaa
    22321 ccagcgcgtg gattgcaaaa ccatgagtcc ctcccgtcag gaggcgttag tctggagtta
    22381 atctaatgtt ggtgcaagtt gaaaaataag aatattcgag cctgcttccc gatgtctgta
    22441 acgccagttc acaagcactc aaaaactgcg atccgtgacg catgttgcaa agataatgcg
    22501 taattatgcc gttacgcttg ccaaacgttc ctgcacaatg gcgatgaaat cgcgtaatgc
    22561 cggcttcatc tcccctttct tccacgccat cagtaaagca atggaaggca catttccggc
    22621 aatgggacga aaaacaacct gcccggtatt aaaattattc atataaccgg gtatcaaagt
    22681 gacgcccagc cccatgccca ccagattcat ggtcaccaga atattcgttg ccacctggac
    22741 gatatttggc tggctgtttt cttgtgcaaa ccacgcttta acgatcggcg caagcgaacc
    22801 ggaatacgcc ggatcggtac tgacgaaatt cacgccatcc agttgcgcgg cggtgatctc
    22861 tttttcatgt gctaaagggt gatcaaccgg taacaccacc actaatggtt cgtcaaaaag
    22921 ctccagataa tcaatctccg ggctataaac aggatggcgc attaagccga cgtcgagttc
    22981 accacggcga attttttcct cctgttgcgt ggtgattaaa ctcaccagct caatcaaggt
    23041 gtctggctgt ctgagacgaa acatcggtaa tacttttggc agtaaattca cttccgccga
    23101 tggcacaaag ccaatggtta attgtctgtc ttcctgaaca attttccgcg cccgtaattt
    23161 ggcattttcc gcttgttcga ggattgccag cgcgtcctgg agaaaacatt ctcccgccgc
    23221 cgtcaacgcg actttgcgct tatccctcac cagtaacgga acaccgacac agttttcaag
    23281 atcgcggatc tggctgctta acgaaggctg tgaggtatgc agtttttccg ccgcacgggt
    23341 aaagttcagt gcctgcgcca ctgcgacgaa atagcgtaaa tgccgtagtt ccatcacctt
    23401 ccccttgtta tcgaaaaaac gtctcagctg gtagaaatta actatttcac atattagcaa
    23461 ccaaccagca acatccttat gacacaaaaa tagaaggtca atacatctta tctttcagga
    23521 ttaaaaaata tgaccacacc ctcagatttg aacatttacc aactgattga cacccaaaac
    23581 ggtcgggtca ctccgcgtat ttataccgac ccggacattt accaactgga gcttgagcgt
    23641 attttcggtc gttgctggct atttctcgcc cacgaaagcc agatcccaaa acccggtgat
    23701 ttctttaaca cctacatggg agaagatgca gttgtcgtgg tgcgccagaa agacggcagc
    23761 atcaaggcgt ttctcaacca atgccgccac cgggccatgc gtgtgagtta tgcagattgc
    23821 ggcaacactc gcgcctttac ctgcccgtat cacggctggt cttatggcat taacggcgag
    23881 ttgatcgatg taccgctgga acctcgcgcc tacccacaag ggttgtgtaa atcccactgg
    23941 gggctaaacg aagttccttg tgtggagagt tataaagggc tgatttttgg caactgggat
    24001 accagcgcac cgggcctgcg tgattacctg ggtgacattg cctggtatct ggatggcatg
    24061 ctggatcgtc gcgaaggcgg caccgaaatt gtcggcggcg tacaaaagtg ggtgatcaac
    24121 tgcaactgga aattcccggc agagcagttc gccagtgacc agtatcatgc tctgttcagc
    24181 catgcttctg ccgttcaggt attaggggcg aaagatgatg gcagcgataa gcgcctcggt
    24241 gatggacaaa ccgcccgccc ggtgtgggaa accgccaaag atgccctgca atttggtcag
    24301 gatggtcacg gcagcggctt cttctttact gaaaaaccag atgctaatgt ctgggtcgat
    24361 ggcgcagttt ccagctatta ccgcgaaacc tatgccgaag ctgaacaacg tttaggtgaa
    24421 gttcgcgccc tgcgcctggc gggtcataac aatattttcc cgacactttc ctggctcaac
    24481 ggcactgcca cactccgcgt ctggcatccg cgcggccctg atcaagttga agtgtgggcg
    24541 ttctgtatta ctgacaaagc cgcctccgat gaagttaaag ccgcttttga aaacagcgcc
    24601 actcgtgctt ttggtcctgc tggttttctc gagcaggatg actcggagaa ctggtgtgaa
    24661 atccagaaat tgcttaaagg ccaccgcgcc cgcaacagca aactgtgtct ggaaatgggg
    24721 cttggtcagg aaaagcgccg cgacgacggc attcctggca ttactaacta tatcttttca
    24781 gaaactgctg ctcgtggaat gtaccaacgc tgggccgatc ttctgagtag cgaaagctgg
    24841 caggaagtgc tcgataaaac cgccgcttac cagcaggagg tgatgaaatg agtgcgcaag
    24901 tttcactaga gttacatcac cgcattagcc agtttctctt tcacgaagcc agcttactgg
    24961 acgactggaa atttcgtgac tggctggcac agctcgacga agagatttgt tacaccatgc
    25021 gcaccacggt gaacgcgcaa acgcgcgacc gccgcaaagg tgttcagccg ccgacaacct
    25081 ggatttttaa tgacaccaaa gaccagctgg agcggcgaat cgcccgtctg gaaacgggca
    25141 tggcatgggc agaagagccg ccgtcacgca cccgccactt aatcagcaac tgccagataa
    25201 gcgaaaccga catcccaaac gtatttgctg ttcgggtgaa ttatctgctt tatcgggcac
    25261 aaaaagagcg cgatgaaacg ttctatgtcg ggactcgttt tgacaaagtt cgccgtctgg
    25321 aagatgacaa ctggcgcttg ctggaacggg atatcgtcct ggatcaagcg gtaatcactt
    25381 cccataacct gagtgtactg ttctgatgaa tcgaatttat gcgtgtcccg ttgcggatgt
    25441 gccggagggt gaggctctcc ggatcgatac ctcgcccgtc atcgccttgt tcaacgttgg
    25501 cggcgagttt tatgccatta acgatcgttg cagccatggt aatgcgtcga tgtcagaagg
    25561 gtatctggaa gatgacgcca cggtggagtg cccgctacac gccgccagtt tttgcctgaa
    25621 aacggggaaa gcgttatgcc tgcccgccac cgatccgctc accacttatc cagtacacgt
    25681 tgaaggtggt gacattttca tcgacttacc ggaggcgcag ccatgagcga tctgcataac
    25741 gagtccattt ttattaccgg cggcggatcg ggattagggc tggcgctggt cgagcgattt
    25801 atcgaagaag gcgcgcaggt tgccacgctg gaactgtcgg cggcgaaagt cgccagtctg
    25861 cgtcagcgat ttggtgaaca tattctggcg gtggaaggca acgtgacctg ttatgccgat
    25921 tatcaacgcg cggtcgatca gatcctgact cgttccggca agctggattg ttttatcggc
    25981 aatgcgggca tctgggatca caatgcctca ctggttaata ctcccgcaga gacgctcgaa
    26041 accggcttcc acgagctgtt taacgtcaac gtactcggtt acctgctggg tgcaaaagcc
    26101 tgcgctccgg cgttaatcgc cggtgaaggc agcatgattt tcacactatc aaatgccgcc
    26161 tggtatcccg gcggcggtgg cccgctgtac accgccagta aacatgccgc aaccgggctt
    26221 attcgccaac tggcttatga actggctccg aaagtacggg tgaatggcgt cggcccttgt
    26281 ggtatggcca gcgacctgcg cggcccacag gcgctcgggc aaagtgaaac ctcaataatg
    26341 cagtctctga cgccggagaa aattgccgcc attttaccgc tgcaattttt cccgcaaccg
    26401 gctgatttta cgggtccgta tgtgatgttg gcatcgcggc gcaataatcg cgcattaagc
    26461 ggtgtgatga tcaacgctga tgcgggttta gcaattcgcg gcattcgcca cgtagcggct
    26521 gggttggatc tttaaggaag tacgatgaaa gaaaaaacga tcattattgt cgggggcggg
    26581 caagcggcgg caatggctgc ggcctcgcta cgccagcaag ggttcaccgg tgagctgcat
    26641 ctgttttccg atgagcaaca tcttccttat gaacgccctc cgctctcgaa atccatgttg
    26701 ctggaagatt ccccacagtt gcagtctgtg ttacccgctc actggtggca ggaaaacaat
    26761 gttcatctgc attccggtgt aaccatcaaa acattgggcc gcgacacacg agagttagtg
    26821 ttagccaacg gcgaaagctg gcactgggat cagcttttta tagcaaccgg cgcggcagct
    26881 cgaccgctgc cgttgcttga tgcactggga gaacgctgct ttaccctgcg ccatgccggt
    26941 gatgccgcca gactgcgaga agttctgcag cccgaacggt cagtcgtgat tgtcggtgcc
    27001 ggaactattg gtctggaact ggctgccagc gccacgcagc gcagatgtaa ggtgacagtg
    27061 attgaactgg cggcaaccgt catgggccgt aatgcaccac cgcccgtgca acgctatctt
    27121 ttacagcgcc atcagcaggc tggtgtgcgc attctgctca ataatgccat tgaacatgtg
    27181 gtcgatggtg aaaaagtaga actgacgctg caaagtggcg agacgcttca ggccgatgtg
    27241 gtgatttacg gtattggtat cagcgccaac gaccaactgg ctcgcgaggc caaccttgat
    27301 actaccaatg gcattgtcat tgatgaggct tgccgcacct gcgatcccgc gatctttgcc
    27361 ggtggcgatg tggcaatcac tcgtcttgat aatggtgcac tacaccgctg cgaaagctgg
    27421 gaaaacgcca ataaccatgc gcaaattgcc gctgccgcaa tgttagggct accgcttccg
    27481 ctactgccgc cgccgtggtt ctggagtgat cagtacagtg ataacttaca gtttattggc
    27541 gatatgcgtg gcgatgactg gctttgtcgt ggcaacccgg aaacccagaa agcgatttgg
    27601 tttaatctgc aaaacggcgt gcttatcggt gcggtaacgc tgaatcaggg acgtgagatt
    27661 cgctcaatcc gcaaatggat ccagagcggc aaaacgtttg atgcgaaaca gctgacagat
    27721 gagaacatcg cgcttaaatc actgtaacca ggataattag cgaatatctc aacgcctggg
    27781 gcgtggcgag gtaccagagt gtgtattacg tttaaatcac attatcttgc aaagggattg
    27841 gttatgaact cactacgtta ttttgatttt ggtgctgccc gccccgtttt gttattaatt
    27901 gcccgtatcg ccgtggtcct aattttcatt atttttggtt ttcccaaaat gatgggcttt
    27961 gacggtacgg tccaatatat ggcctcgctg ggcgcaccga tgccgatgct ggcagcgatt
    28021 attgcggtag ttatggaagt gcccgccgcg atattaatcg tgcttggctt tttcacccgt
    28081 ccgctggcgg tactgtttat tttctacacg ctgggtacgg cggtgattgg tcaccattac
    28141 tgggatatga ccggcgatgc cgtcgggcca aatatgatta atttctggaa gaatgtcagt
    28201 atcgctggcg cgttcttgct attggcaatt accgggccgg gggcaatttc tctcgatcgc
    28261 cgttaggaaa gatgccggat gcggcgtgaa cgccttatcc ggcattttat aaattacagc
    28321 cactctaccc gcaacgacat ctctgaggtt gttgattccc ccggcgcaag cgcaatgagg
    28381 tcaccccctt ccgggcgatg atggtcatcc ggcgcatggc tcatcggctc cagacagaag
    28441 aaatcaaacg catatccttt atcaaacgcc gggtcggaaa caaagatgaa ataacacggt
    28501 gcaggtggcg tcgtttctat gatgatggca taaccctcct gcggctgctc gatgcgggcc
    28561 tgaccattcc atccggcaaa accattgttc acccactggc gcggcaacgg cgctagctgg
    28621 ttaaaatcca gttcttgcgg tagctgctcg caaaactcac ccgccagcca ctgctcccgc
    28681 tccagccagt aaccgctcgc ctgtgcctga atccgcgttt gcggcgacaa cggaaaataa
    28741 ggatgccagc cggtaccaaa tggcagcgtc tctgcccctt gattggtgac agagagcgtc
    28801 accgtcagcg tatccgccgt taaatgaaac gcctgactta cccgatagtg atagacaccg
    28861 ctgcgatgtt catacaccag acacaaacta tcatcgctgt gcgagacaca ttgccactcg
    28921 cccagccagc catcgccgtg tagatagtgc gcatcccact caacattcgg ttgcagttga
    28981 tactcacgcc cctgccagac aaaacggttg ccgctcaccc ggtttgcaaa tggcaccagc
    29041 ggaaaacaag aggcatcggt tgccacaccg ctttttttac caggacgtaa gagcggcgtc
    29101 gtatcgcgcc agaagccttc gattacgccg ccctggtcag aaacgcccag ctttagcgac
    29161 ccgtgggata aggtatagat ggtcatccgc taactcctta atccgggaag ttaatcacaa
    29221 ctttcccgca tttgccgctc gccatcagcg cataggcatc acctgcctgt tccagcgaga
    29281 agcgatgggt aatggcgtta cgcggccaca gcttccagtc agtcaaatcg tgggcgcatt
    29341 tttccatatg gaacagactg gtcacccagg agccaataat ccgccgttga tggtgcatca
    29401 gatcggcgct gacctcgaat tccacttttc cggtttcacc aatgtaaacc acccgccccc
    29461 agtcagcggt ggattgcagt gccagtaagc gacctgcggc attaccggaa caatcaagcg
    29521 caacatccgc gccgccgtga gtgagttcgg cgattatctg cggcagacct tcagtggtcg
    29581 ctaaatagcc gtgatccatc actcctaact gttttgccat cgccagacgt tccggcagca
    29641 tatcaacgcc gataatccgt tttgcaccgc gacctttcgc cagcatcatc cccatcatgc
    29701 cgactggccc cagaccgacc accagcacgt tatcactgcc ggaaacttcg ccgcgcaaaa
    29761 tcccttcata ggccgtgcca acgccgcaac tgataaacgc accatcttcg tagctcagcg
    29821 catccggcag gaggatcaga tctttttctt ccgccagcaa gtattcggca tgaccgccgt
    29881 cacgctgcca gccgtaagcc gcttttcctt cgccagtaca agaaatagga aaaccgcgac
    29941 ggcagttcgg gcaaaaacca cagccagaaa tgtgatacac cagcacgcgg tcgccctctt
    30001 taaaatggcg gcagccttgc cccatcgcca caatctgccc gcacggttca tgaccgttga
    30061 taaatcccta gtacaacggt ttatcggatg ccgccgccgt agcgcggtgc tggtggtaga
    30121 tatagtggac atcgcttccg caaattccgg aggatttcat tttgatcagt acctggttaa
    30181 tccccggcgt cggcactgca acttcccgca gatcgacggt cgaatttcct ggtaaataag
    30241 ctgccagcat cgttttcata aatccctcat taacaatacg attaattttc atccctgccg
    30301 cacccgcgcc aggggcgtta aggtttagcg tttcgcttta ctgctacgct gggtcagcaa
    30361 gatattcgcc agcaccgcca ccacgatgat gacgccgcgc accacctgct ggaaaaagga
    30421 gttaatcccg agcagcacca gaccgttacc gattagcgta atcaccagta cgccaagcag
    30481 cgtaccgaac aacgagccgc gaccaccgga aagcgccgta ccgccgacca cgaccgcggc
    30541 gatgacgtca aactccagac cgtttgcggc acctgcgtta ccggaaccga ggcgcgccgc
    30601 caacaaaatg ccggtcaccg ccgctaataa tcccgaaagg gtaaagataa gaatgcgtac
    30661 ccgacgaacg ttaataccgc acaactgcgc cgccgtggca ttaccgccaa cggcaaaaac
    30721 cgagcgcccg aaggcggttt tgcggctaat gaacacaaac agcgcaaaca gcaccatcat
    30781 gatcagcgcg gataccggca caccgagaaa ttgtccgccc agccagtcca gcacctcgtt
    30841 ttcgtcaatc ggcactggca gagcgttggt cataaacaag cccattccgc gcagggcgct
    30901 ccacagcccc agcgtggcca cgaaacttgg cacgttaaac acgccacgca gcaccccggc
    30961 gagcgttccc atcagcgcgc ctaacagcaa caccagcaga caagcaatcg ccaacggaac
    31021 ttcaaattgc agcaaaaatg ccaggcatac cgagacaaaa gccaccatcg gcccaacgct
    31081 gacatcaatt tcaccggaga taataatcag cgtcatcgcc caggcggcaa tcccaatggt
    31141 ggcggcatcg cgcagcacgt tcatctggtt attcaatgag ataaagccag gcgcgttcag
    31201 ggagaagacc agataaagaa tggcaatcac caccagcaaa ccgatctcat taatatggtg
    31261 actgacaaat tgtttgagcg agacgctctt gccctgcggc aatggtaatg acgaagcaga
    31321 catcttcagg ttcctcggta aaattcatca gtgcacagac agaatggcgg acatcagctc
    31381 atccacattg accggagcgt gaaactcctg cgagaacgtg ccgtgctgta ataacaggat
    31441 gcggtcacac accaacggca actcctccac ttcactggag ataaacacca cgctttttcc
    31501 ttcggcagcc agctcacgga caatacggta aatctgctgt ttggcttcga tatcgacgcc
    31561 gcgcgttggc tcgtcgagca acaaaatctg gctggcggca tagacccaac gaccgatcac
    31621 tactttttgc tgattgccgc cagaaagcgt gccgatgggc gtttcgctac tggcggcctt
    31681 gaccgtcatc cgctgcatca cttcttcggt caggcggcgg atggtggacc attgcagcac
    31741 tccgttggcg ctgatttttt gccgattggt cagcactgta ttttcgtcaa cgcccaacca
    31801 gggaatgatc cccgcttctt tgcggttttc tggcgtatag ccaatgccgc gtttcagcat
    31861 gtcgccgtaa tcggggcgcg tgattttctc gccgttgata acaatttcgc cctgttcata
    31921 cgtctccagc ccaacaatcg ccttcagcaa ttcactgcgc cctgccccca gcagaccagc
    31981 aatgccgagc acttcgccac gacgtagcgt aaagctgata tcctccagct tgggcttatg
    32041 gcgtaacgca cggacttcca gcacggcctg atccacaatt tcctgaggtg caaccggcgc
    32101 aatatcaacg tgatcgcgcc ctagcattag cgacacaata tgatgcgtgg aagtgttttc
    32161 gagcatcaca tcgccagcca cctgcccgtc gcgcataacg gtggcactgg aggcaatgcg
    32221 gcgaatttct tccatccggt ggctgacata aatcaccgcc acgcccagtg ctgacatctt
    32281 tttcaccgct ctgatcacca gttcaacttc cgcactcgca agcgaactgg taggttcatc
    32341 aagaatgacc acgcgcggct cgcccttcat cacccgcgca atttccacca gctgcttatg
    32401 cgccgggctt agtgttgaaa caagttgttc aggactaacg tcaacgccca gcgcctgtaa
    32461 gcaacgttgg gcatcctgcg ccatttgcag gtaatcaatc atgccgttgc ggcggggcca
    32521 ctgaccgagg cagaggtttt ccgccaccgt cagcccttcc accagactta actcctgata
    32581 aaccgcgcgt acccccagtt cagcggcacg gcgagtcagc gtagcttcgt caccttccag
    32641 tcgcgtctcg ccaatccaga tttcaccgct atccggacgt tcactgccgg taagcattcg
    32701 aatgagggtc gatttgcccg cgccgttttt gcctaacagc gcacgaacct cgcctttatt
    32761 gagcgtgaag ttaacgttat ccaacgcaac gacgccggga taacgcttat ttcctgccac
    32821 cacttttgct accgggactg cctctgttgc cgtgaacata gggacctctg cgaatcagcg
    32881 attagggcag accatcaacg tgcgttgcca gccactgttt accgtcttcc gttttggtat
    32941 agagatcgat aggcacctga atcacttttt caccgtcggc ttgtttattg ataaccttca
    33001 atgtttgcgc gaaaacagca ttgcccattt tcttaccgga aatatccact accgctttca
    33061 gcacctgatt gttttccagc tcctgagcaa tttcggttgt catatccgaa ccgaaaacag
    33121 caatttttcc ggcctgattt tgattacgta ccgcttttac cgcaccgagt gtcgcaccgc
    33181 ccgattcccc cataatggcg ttgagatccg gcgtggagat aatcagtttt tcaccaacgg
    33241 aaatcgcttt atctaaaaca gtcccttcct gattagcgac aatttgcgcg ccgggaacgc
    33301 gggattttaa tacttcttca aatcctttac gtcgctgcac acaaacttca aaggcttcgc
    33361 aattgatgac ggcaattttc ggctggtcaa ttttattggc aataaaataa tcggcggcag
    33421 cgttacccag ttttttacca aattccagcg ggtcacccac cagatacgcc gagacatatt
    33481 tatcgacccc cttttgatta atacaggtgt tgtagcaaat caccggaata cccgcttcac
    33541 tggcgcgacg aacggtacgg ctactgccat tttcagacac tgccgataaa ataatagcat
    33601 cgacattacg cgccacgagg gtatcaacaa aggtactttc tttcgaaata tcaccctggg
    33661 cgttagtttc aattaactgt acctgtactg aagaatcttt tgccgcatcc tgaactccct
    33721 gacgcactcc agcgtaatat ccctgagtat caaggtatat tgcgccaatg gtcatttctt
    33781 tatccgcagc tctggcaaat agtgcgctac ctaatagcgt agccattaat aatagattac
    33841 gggttgttct cattttttta ggcatagagc ctcctgtagg gtttttatta acaacggctt
    33901 attctaatta ttttgtgatg agcggcagcg cgtgccgctc aaatattaca gttaataggg
    33961 tttagtgaac gtagttaaaa ataaaaggca tcacggtggt gaataatgcc gctttcggcc
    34021 aggccggatt aatcgccgtc aattcagcgc gagcagattc gtattgcgca atctcgccta
    34081 atcccgcggc cgccagcatc gccaccatca ggcatttttc tttatgctgc tgttgtaaat
    34141 cgccatacag cgacaacaag tcaggctgcg agacggcaaa gaaatccgct tcgatactgg
    34201 ttttcgccat ctcttgcgcc cactgtttca tttcactaaa cagttgctgt gcggtgtgtt
    34261 gttcgcccag taatcgcagc gccattcctt gccagaagag gtaatcaacc ggctgatcgt
    34321 tgtaatagct gtggatgtta atggtgcgat cgccggtcgc cgccagatgt aaacagcgcg
    34381 tcgcttcagt ttcatcgccc tgggctttgg cgcatatcgc ctgccagaac cagatgtcgt
    34441 tatcagtttg ccccggtaaa cggccttcgc ttaaattctc cggataatgc agcgcggcat
    34501 gaagcagttc gctggcctgc tgcggctctc tggcatcaag atgctgccag gcgcgtaata
    34561 actggttgag gataaactga ctggtgacct tcccttcccc gccttcccac gggtggaatt
    34621 tgcgcgtggc gagaatgtcc gccgctttgt ctgcctgccc cgtgagatgc cacaaattga
    34681 gcagttctgc ggtcatgtcg tcgcgtttca gcgcaatttc cagattattt tccagacgcg
    34741 ccagtcgttt ctccggtgtg gttccactta gcttatcaag caaatcccgt tcgaaaagca
    34801 gacgtgcatc ctgcggcgca agctgataag cattatcaag ataacgcgcg gccagctcat
    34861 aatcgtgttg cttattccac gcatggatcg ctaacccgcg ccagccgtcg gcaaactccg
    34921 gcgacatctc tacgcaacgt tgccataagg caatggcttc gttgtagcta cgtttgttgt
    34981 agtagaagca ggccagtaaa tggcgagcaa accagcactc ttcaatactc tccagcgccg
    35041 ccacttcttc cagcgtattc gggaaacgga caaactgcgg gaagacatca atggcttttg
    35101 cgaccagttc gccacgttcg gctttcggca gcaagctggc ttgcaggtaa agcggcagcg
    35161 tgcgctggca gtccagtgca ttcagcatct ctgccgcgag ggtgggcatt ccccagttaa
    35221 tcagttgccc ggcggtcatc agggcgttaa cgtcgcgtcc ctgacacaga ccgcgccact
    35281 gcgcgagagc tgattcgcta cgaccatcga accagttcag gcaccacaga gtggcgttca
    35341 gcggataatc gcgcagcagt ttctcgcgct gcaaacgcgc gttgtcctga cgaccactta
    35401 acaccagcag cagattatgc aggcaaagca cttcctgatt gattgggcag gcgcgaagac
    35461 tttgttggca aaaatccaga cccgcgtcga agttaccatt acgcgccgcc agtcgtgcca
    35521 gaccataata gcctccggct ttactgttgc cgctccagac cgcacgccag aaatcctctt
    35581 cggcttgttg atattgtccc tgacgttcgt aagcactggc gcgaatcaaa ctcgcctgtc
    35641 cgcactgcgg atttttgttc agcgcatgtg cgcgtttcag agcctgagtg gcatacgcca
    35701 ccgcttgcgg gaaatctgcg cggttatact ccagcatcgc cagcgccaga ttacagcggt
    35761 aatccagcgg atccagcgcc acgccgcgca ggtagtaatc gaacggtgaa cggctggcgt
    35821 gatgatattg ctccagatgc tgaccgataa accaggcttc atctgtactg gtaatgtctt
    35881 gtgctgacag tggcgctttg gcgacgtccg gcaacggcaa ctcttgcggc tgatgttcct
    35941 gataactcag tacaatattg ccgtcggcat cagagagctc aatggtcagt ctttcaggat
    36001 tgataccgtg taacacgccc tggatggcgg tcgctggtgt cagggccacg gcatcatcga
    36061 gcaacgcgtc gcatttgccg atttcgcgga tcgccaggcg gtatccgttc aacggagaga
    36121 tggcatacag cccccactca atcccccgct cactacgctg gagtttgatt accgcatcgc
    36181 gggaggcgtt ttgcaccatg cccagagaat ggtaaggcag gaaaaactgc tcaaaacgct
    36241 tctcttcgta agcatcaagc cgggtaaaat caggctggtt atcggcaaaa ataccggtca
    36301 tcagttcgat atacgggccg ttattatcag ttaggctctt atcccacgcc tggccaaatt
    36361 cactgtgtcc ccagctccac tgctttttac ctggcgcaat atggtggttg gcaacgtgta
    36421 gcaaaccgcc atcttcatcg tgacaccacg cgccaacaaa atcgtactgt gatttttcag
    36481 ccatatatga ggttggaacg ggcacatttt tatagcgaga aatgtccact ccagcggagt
    36541 agtccacttt gtagtaagtg ccggtggcga tagggaaagc ggagacggcc cgtttgccgt
    36601 gatcaaacac cgccgttaca tccggcggaa agacgctctg atgcccttca ccccctttca
    36661 ctgccgggtt ggcccaccac aagaaatgac gcggcgtggc gttgccgtta tagacgcggc
    36721 tggcgatttc cagcgccgcc cggtcagggc gcagggtgaa acctgtcatc acctgtaaac
    36781 catgcatcgg ctccgtttcg cctacccaca ccgtctgtgc gccgtcttca tgggcttcga
    36841 gggtgaaatc aacgggcata aaggtggtcg ggcgatggtg ttgcggccag ttaaactcaa
    36901 tcccgccgga gatccacggt cccagcagcc ccaccagcgc aggtttaatg acttcattgt
    36961 gatagacaaa atcgcgctgt ttcactttat cccatgcgcg atgcacccga ccgcccaatt
    37021 ccggcaggat catcactttg atgtagtcgt tttccagcca caccgcctgc caggatttca
    37081 gggttttctg ctcgctcagc gtatcggtca cgccgtaggg ataaaccgcg ccggacgatc
    37141 cctgataaac gcgattttcc aggaacatgg gatgtatatc ctgcggcccg gtttcatagg
    37201 tcgggatctc aacgcgctct tgccacactt ttactggagt catggtgccc tcaatattaa
    37261 caagacatac tgaattaaaa gatttgtggc agtgtattga acaatctggc aatgttttcg
    37321 cggaataatc acgcaattaa ctaaacaagg tttagtgaag atgagagcct gcattaacaa
    37381 tcaacagatt cgccaccata acaaatgcgt gattctggaa ctgctgtacc ggcaaaagcg
    37441 cgccaataaa tcaacgctgg cccggctggc gcaaatttcg attccggcag tcagtaatat
    37501 tttgcaggaa ctggaaagcg aaaaacgggt ggtgaatatc gacgatgaaa gccagacgcg
    37561 cgggcatagt agcggtacat ggctgattgc gccggaaggt gactggacgc tgtgtctgaa
    37621 cgtgacgccc accagtattg agtgtcaggt tgctaatgct tgtttaagtc cgaaaggtga
    37681 atttgagtat ttacagattg atgcaccgac gccgcaggcg ctgctgtccg aaatcgaaaa
    37741 atgctggcat cgccaccgta aattgtggcc ggaccgcacc atcaatctgg cgctggcaat
    37801 ccacggtcag gttgatccag tgactggagt gtcgcaaacc atgccgcaag cgccgtggac
    37861 aacgccggtt gaggtgaagt atctgctgga agagaagctg ggcattcggg tgatggtcga
    37921 taatgactgc gtgatgctgg cgctggcgga aaaatggcaa aataattcgc aggaacggga
    37981 tttctgcgtg atcaacgttg attacggcat tggctcgtcg ttcgtgatta acgagcaaat
    38041 ttatcgcggc agtttgtatg gtagcggaca gattggtcac accatcgtta atccggatgg
    38101 cgtcgtctgc gactgtggac gttacggctg cctggaaacc gtcgcctcgt taagtgcatt
    38161 aaaaaaacag gcgcgggtat ggctaaaatc acaaccggtt aatactcagc ttgatcctga
    38221 aaaactgact acagcgcagt taatcgctgc ctggcaaagt ggagaaccgt ggatcaccag
    38281 ctgggttgat cgctctgcca atgccattgg tttgagtctg tataacttcc tcaacatcct
    38341 caatattaat cagatttggt tgtacggtcg cagttgtgcc tttggtgagc actggcttaa
    38401 tactattatt cgccagacag gatttaaccc gttcgaccgc gacgaaggac cgagcgtgaa
    38461 agcgacgcaa attggcttcg ggcaattaag ccgcgcacaa caggtgctgg gaattggcta
    38521 tttgtatgtt gagacgcagt tacgacagat ttgatggcgc gataacgtag aaaggcttcc
    38581 cgaaggaagc ctttttcaac agattaaccg tttcgcttat gcgtaaaccg ggtaacgtgc
    38641 gcagatgtcg agaactttac ctttgatgcg ctcgataacg gcttcatcat tgatgctgtc
    38701 cagcacgtca cacatccagc cagccagttc tttcgcttct acttctttga agccgcgacg
    38761 cgtaattgcc ggagtaccca cgcgaatacc ggaggtcaca aacgggctct tcggatcgtt
    38821 cggtacgctg tttttgttga cggtgatgtt agcacggccc agagcggcgt ctgcttcttt
    38881 accggtcagg tttttatcga ccagatcaac caggaacaga tggttatcag taccgccgga
    38941 aaccactttg tagccgcgtt ccaggaacac ttctaccatc gctttagcgt ttttagcgac
    39001 ctgctgctgg taagttttga actcaggctc catcgcttct ttcagagcaa ccgctttacc
    39061 ggcgattacg tgcatcaacg gaccgccctg accaccaggg aaaacggcag agttcagttt
    39121 tttgtacagc tcttcgctac cacctttcgc caggatcaga ccgccgcgcg gacccgccag
    39181 ggttttgtga gtggtggtag taacaacgtg agcatgagga accgggttcg ggtagacgcc
    39241 agcagcaacc aggcccgcaa cgtgcgccat atcaacgaac aggtaagcgc cgatgctgtc
    39301 agcgatttca cgcattttcg cccagtccac cacgccggaa tatgcagaga agccaccgat
    39361 aatcattttc ggtttgtgtt ctttggcttg tttttccaga tcggcgtagt cgatatgacc
    39421 ggtagcatca ataccgtaag gaacaatgtt gtacagttta ccggagaagt taaccggaga
    39481 accgtgagtc aggtgaccgc catgcgccag gttcataccc agaacggtat cacctggttc
    39541 cagcagcgcg gtgtagaccg caaagttagc ctgggagccg gagtgcggct ggacgttagc
    39601 gtagtcagcg ccgaacagtt ctttcgcacg atcgatcgcc agttgttcaa cgatatcaac
    39661 atactcgcaa ccaccgtagt agcgtttgcc cggataacct tcagcatatt tgttggtcag
    39721 ctgagaaccc tgcgcctgca ttacgcgcgg gctggtgtag ttttcggagg cgatcagttc
    39781 gatgtgctct tcctgacgta ctttttcctg ctccatagcc tgccacagtt cggcatcata
    39841 atcggcaatg ttcatttcac gctttaacat ccgcatctcc tgactcagct aacaataaaa
    39901 tttttggcct ttataggcgg tcctgttgga caacggcgaa cagtataacc gaatcattgt
    39961 gcgataacag gtcttgacaa aggaatttac gcaaacgatt accttcaggc tacgcaaggc
    40021 tttggagaat aaagagcttg caaccggaaa cggatttctt ttcaggtttg tgatgcaaat
    40081 ttttcacttc atcacattct ttctgaaaaa caccaaagaa ccatttacat tgcagggcta
    40141 ttttttataa gatgcatttg agatacatca attaagatgc aaaaaaagga agaccatatg
    40201 cttgacgctc aaaccatcgc tacagtaaaa gccaccatcc ctttactggt ggaaacgggg
    40261 ccaaagttaa ccgcccattt ctacgaccgt atgtttactc ataacccaga actcaaagaa
    40321 atttttaaca tgagtaacca gcgtaatggc gatcaacgtg aagccctgtt taacgctatt
    40381 gccgcctacg ccagtaatat tgaaaacctg cctgcgctgc tgccagcggt agaaaaaatc
    40441 gcgcagaagc acaccagttt ccagatcaaa ccggaacagt acaacatcgt cggtgaacac
    40501 ctgttggcaa cgctggacga aatgttcagc ccggggcagg aagtgctgga cgcgtggggt
    40561 aaagcctatg gtgtactggc taatgtattt atcaatcgcg aggcggaaat ctataacgaa
    40621 aacgccagca aagccggtgg ctgggaaggt actcgcgatt tccgcattgt ggctaaaaca
    40681 cctcgcagcg cgcttatcac cagcttcgaa ctggagccgg tcgacggtgg cgcagtggca
    40741 gaataccgtc cggggcaata tctcggcgtc tggctgaagc cggaaggttt cccgcatcag
    40801 gaaattcgtc agtactcttt gactcgcaaa ccggatggca aaggctatcg tattgcggtg
    40861 aaacgcgaag agggtgggca ggtatccaac tggttgcaca atcacgccaa tgttggcgat
    40921 gtcgtgaaac tggtcgctcc ggcaggtgat ttctttatgg ctgtcgcaga tgacacacca
    40981 gtgacgttaa tctctgccgg tgttggtcaa acgccaatgc tggcaatgct cgacacgctg
    41041 gcaaaagcag gccacacagc acaagtgaac tggttccatg cggcagaaaa tggcgatgtt
    41101 cacgcctttg ccgatgaagt taaggaactg gggcagtcac tgccgcgctt taccgcgcac
    41161 acctggtatc gtcagccgag cgaagccgat cgcgctaaag gtcagtttga tagcgaaggt
    41221 ctgatggatt tgagcaaact ggaaggtgcg ttcagcgatc cgacaatgca gttctatctc
    41281 tgcggcccgg ttggcttcat gcagtttgcc gcgaaacagt tagtggatct gggcgtgaag
    41341 caggaaaaca ttcattacga atgctttggc ccgcataagg tgctgtgatt tgatgttgcc
    41401 ggatggaaac atccggcaac ccttgacgcg gtttaaattg ccgcgtcgtc ctcttcaccg
    41461 gtacggatgc gaatgacccg tgccacgtca aagacgaaga ttttaccgtc accgattttg
    41521 ccggtttgcg ccgtgcgaat aatggtatcg acacaggtat cgacaatgtc gtccggtacg
    41581 acaatctcaa ttttcacttt cggcagaaaa tccaccatat actccgcgcc gcggtacagc
    41641 tcggtatggc ctttctggcg gccaaaacct ttcacctcag tgaccgtcat gccggtaatg
    41701 ccgacttcag ccagtgcttc gcggacatcg tccagcttga agggtttaat aatcgcatca
    41761 atctttttca tgctattcct tgaaaaggtc gcctgtcttt tgatctgcta aacgtaacac
    41821 ataacgccaa ttcattcctt gaaatcgttt gcatccagct cgtgtcggga aagcagttta
    41881 taaaattctg tccggttgcg ccccgccatt ctcgccgcgt gggtgacgtt gcctttggta
    41941 atttgcagca gcttacgcaa atagttgagt tcaaactgat tacgtgcctc aacaaaggtt
    42001 ggcagcgccg tattttcacc ctccagcgcc tgctctacta atgcatcgct aatcaccgga
    42061 gatgaggtca gcgccacgca ctgttcaatc acgttgacca actggcgcac attacccggc
    42121 cagctcgcgg tcatcaggcg tttcatcgca tcggtggaaa acgcgcggac aaacggttta
    42181 tgtcgctctg ccgcctggcg caacaggtga tttgccagta gcggaatgtc ttctgtgcgc
    42241 tccgccagtg ccggaatttt caggctgaca acgttgaggc ggtaatagag atcttcgcgg
    42301 aattccccgc gcgccatcgc ttttggcaga tcacggtgag tggcagaaat aatccgcaca
    42361 ttgatatcaa tatcgcggtt actgcccagc gggcgcactt tacgctcctg caacacgcgc
    42421 aacagtttga cctgcaacgg cgcaggcata tcgccaatct catcgagaaa tagcgttccg
    42481 ccttccgccg cctggaataa accttcgcga ttgctgacag cgccagtaaa cgcgccacgc
    42541 gcatgaccaa acagctccga ctccagcaat tgctcaggca atgcaccaca gttaatagca
    42601 ataaatggtt tgctattgcg cgggctggcg ttgtggatag cctgggcgaa aatctctttc
    42661 ccggtgccgc tctggccatt aatcaaaacg ctgacgtctg attgcgccac cagccgcgcc
    42721 tgttccagca aacgcagcat cagcgggctg cgggtgacaa tcgcctcgcg ccagcgttcg
    42781 tcggtggctg gcgcggattg ttccagcgca tcatcaattg cctgatatag cgcgtcttta
    42841 tcaacaggct tggtgaggaa actaaaaacg ccctgctgtg ttgcagcaac ggcatcggga
    42901 atagaaccat gcgcggtaag aataattacc ggcattcccg gctgcacttt ctggatttca
    42961 gcaaacagct gcataccgtc catttcatcc atccgcaggt cgctgatgac taaatctact
    43021 ttttcgcgat tcagtacccg taatccttca gcgccacttt ccgccgtgac cacgctgtag
    43081 ccttcgctgg tcaggcgcag gccaagcagt ttcagcaatc ccggatcgtc atcgaccaat
    43141 aataaatgcg caggtttatg gctcatcagg agtgacctca tgggtggatg gcgcgggctt
    43201 ttcactctcg tgtggcgtat ccggcgagaa atttccagcc ggtttgcggg tcgagagctg
    43261 gcgttcaata tcagtcaggt tttccagctt gcgggtggtg agttccagtt gctgctgtag
    43321 aacgtgatgt tgctggcgca atgtatccag ctcgctgtcg ctggactgct ggagtttact
    43381 gtagcgttgg cgctcttccg ccagttgcag ttgcagcgcc tgaccgtcgc gccagagttg
    43441 atacagtggg cgaacctgtg ccgggatctc ggtacttaac gcttcaatac gcgcgaccag
    43501 ctggcggcgc tcatatggcg taattttggc gtcggcgagc aaaatccctt gtttaaaggt
    43561 attttgccag ctgccgtcgt catattggcg agcttgctga cgcgactgcg caggcattaa
    43621 acgatcagca caatccatcg cccgcagcca gtaaagcgga ttggtttcgg ttgatttacc
    43681 ttgcagcgcc cagatgtcgc tacattcagt agaaagatag tcagccagtt gataaaccgg
    43741 aattttttct tctgctggcg tatcaatggc tggcttattg tgattctgca cgcaacctag
    43801 caatgccaga catggcagcc ctgccagcca cagccgtcgg ggcaataatc gttgaaaaat
    43861 gtgtcgcata ttcaccagac ttaaagccta tcccagtggg cgtaattgtt gcagacagtt
    43921 tggacatgga cagcgcggag aaaccggagc gtacatgtcg tacgtgagga tttcgagcac
    43981 tgcccggggc cgaactgaca aataaaatag cctgatggga taggctgtta gcattatttc
    44041 gtgtttttcg acgacggtaa ttcaatgcgg aaacaaacgt cttgcccgct ctcgtcgacc
    44101 agatacagtt ccccttgcat acggcgaata caatccctgg caatgcttaa tcccagaccg
    44161 ctgcccttca ccgccccttt tcgctggtgg cttccctgaa aaaagggttc gaagatcatg
    44221 gcgcgttcct cttgcggaat gggcgtgcct gtattgatga catcaatata aacccgcgca
    44281 ccatgtaaac tgctgcgaag gcaaatgtta ccggattcag ccccgtagtg caccgcattg
    44341 gagtaaagat tatccagtac gctcatcagc agcattggct ccgccaggca agctgttgct
    44401 ttgagatcga cgtcggtatg catcatttta gcccgtgcgg gcaggctatg agcagaaact
    44461 actgtctcca ccagcggtgc taactcaaca ttatctagtt ccaccgcact gtccgcctgt
    44521 ttacggttgt aatcaagcag ttgttcgatc agtttttgca aattgcggct gctgctatca
    44581 agaatgctca ccacctcttt ttgctctggc gtaagcggcc cgacaacctg atcagccagt
    44641 aattcagtgc cctcgcgcat actcgccagt ggcgttttta attcatgaga taaatgtctt
    44701 aaaaattgat ggcgttggga ttccagccat gacaggcgct cacttaacca aagaatacgt
    44761 tgcccaaccg agcgtaactc gctcggtcca ctgaacgaaa cgctattgcc cagagaacgc
    44821 ccttccccca accgattgat catccgttca atatttttca ccggcccgat aatcatccgc
    44881 gtgaaaagca gtaccattac cagactcacc agaaatagca ccagcgattg ccaaccaaaa
    44941 tattgcccac gttcggcgat ttcacgctga agttgctgcc cacgagagaa cactactgtg
    45001 cgcgtggcct gtaccatttc ggtattggca ctggcaaagg cttccagacg cgcggcagca
    45061 gcagcatcgg gaccactgtt gttgcactga agttgggcca gattgttcaa gtcctgacgc
    45121 aatgcctggt aaagtttatc gtctggcagt acgcctgcgt gggcatcgag catttcgctg
    45181 taacgcttgc gctggctttg ataaaccttc gcaagcgtag ggtcatccag cacgcaatac
    45241 tgacggtagc tacgctccat ctccagcgcc gcgttggtca ttgcttcgct gcgccgggca
    45301 tcgataagcg tggtgcggtt gaccagcgcc gcctggtcgc taagcgcatt caggctttgc
    45361 caggcttgcc atgccagcac caacaggggc agcagaatca gcaaaaatgc cagcattacc
    45421 agttgtcgta atgagcgggg aaaaacgggc cagcgtttca aggtgttact ctcgtcagac
    45481 gcgaatagcc tgatgctaac cgaggggaag ttcagataca acaaagccgg gaattacccg
    45541 gctttgttat ggaataaggc ggtgcctaac tcgacgtttc gcccgatggt tgatatagct
    45601 acgctgatat cagaagttgg acggcaggca ccttgttgtg cgtcattcga attttatgta
    45661 gcacgtcccg aaggggctga cataagtcgg tgaatgagcc actgattact attatgcagt
    45721 aactgtgcca ataaagaaaa tagtttggta acgtactgat tatacgttgc tttgaggggt
    45781 ttatggcttc cccgctattt gaatgtaaat cagccactgt gtcgctaaaa agagacaact
    45841 taagaaaaac ttattagatt acaataaaat caatcattta agtgtcgcca gaaagcgaca
    45901 cggtaaacca cccattgtcg tgatttacag acacaaaaaa gctcccgaag ttgggagctt
    45961 ataatagcgg ttgtatttaa tgccggatgc ggcgtgaatg cctcatccgg gctgcaaaac
    46021 caatgagttt gacggcttac cccaactgct tacgtgcatt gcggaaaatg cgcatccatg
    46081 ggccatcctc gccccagttt tcaggatgcc aggagttgct gacagtacgg aaaacacgtt
    46141 ccgggtgcgg catcataatg gtgactcgac cgctttcagt cgtgaccgcc gtaataccgt
    46201 tcggagaacc gttcgggtta gccgggtagg tttcagtgac tttgccgaag ttatcgacat
    46261 agcgcagtgc caccagccct ttgctttcca gtgccgccag atgcgccgca tcacgcactt
    46321 ccacgcgccc ttcaccatga gagacagcaa tcggcatttg cgagcccacc atcccctgca
    46381 acagcagcga cgggctttgg gtcacttcaa ccaagctgaa acgcgcttca aagcgatcgg
    46441 aggtattgcg cacaaaacgt ggccacaact cactacctgg gatcagttca cgcagattag
    46501 acatcatctg gcaaccgtta cataccccca gcgccagcgt ttgcggacgg tggaagaagg
    46561 ttgcaaactc atcgcgtaca cggtcattga acaggataga cttcgcccaa ccttcaccgg
    46621 cacccagcac atcaccgtag gagaaaccac cgcacgcgac cagggcgtgg aaatcctcca
    46681 ggcccgtgcg tccagccagc aggtcgctca tatgcacgtc gatagcatcg aagcctgcac
    46741 ggtggaaagc tgccgccatt tcaacatgcg agttcacgcc ctgctcacgc agcacagcaa
    46801 ctttcggacg tgcgccagtg gcaatatacg gtgctgccac atcttcgttg atatcaaacg
    46861 acagtttcac attcaggccc ggatcggcgt cgtttgattt cgcctgatgc tcctgatcgg
    46921 cacactccgg gttgtcacgc aggcgctgca tctgccaggt agtttctgcc caccagacac
    46981 gcaacgtagt gcggctttcg ctgaatacag tctgcccgtt ggcggtaatc acaaaacggt
    47041 caccggaaac cgcctgccca acataatgga cgcaatcagc aagcccatgc tgtgccagta
    47101 cggactcgac cgcttcacgg tcagcggcac gaacctgaat gaccgcaccc agttcttcgt
    47161 taaacaacgc cgccaggcga tcgtcaccca gagtggcgat atccgcgtca atgccgcaat
    47221 gaccagcaaa ggccatttcc gccagcgtta ccagcagacc gccatcagag cggtcgtgat
    47281 acgccagcaa cttacgctgt gcaaccagcg cctgaatcgc gtcatagaag cctttcagtt
    47341 gcgcgacatc gcgtacatct gccggtttgt cgccaagctg acgataaacc tgcgccagcg
    47401 ccgttgcgcc cagcgcgtta ttgcctttgc ccaaatcaat cagcagcagt gcgttatctt
    47461 cggtagaaag ctgcggcgtg atggtgtgac gtacatcttc cacgcgggca aatgcagaaa
    47521 tcaccagcga cagcggcgac gtcatttcgc gctcttcgtt accttcctgc cagcgggttt
    47581 tcatcgacat agagtcttta cccaccggga tcgtcaggcc cagcgccgga caaagctctt
    47641 cgcccacggc tttaacggct tcatacaggc ccgcatcttc accagggtgg cctgccgccg
    47701 ccatccagtt ggcggaaagt ttaatgcgtt tgatatcgcc aatctgtgtt gcggcaatat
    47761 tggttaacgc ttcaccgacc gccagacggg cagaggcggc gaaatccagc agcgcaaccg
    47821 gcgcacgctc gccaatcgcc atcgcttcac cgtagtagct gtcgaggctg gcggtagtga
    47881 ccgcgcagtt agcgaccggc acctgccacg gccccaccat ctgatcgcgc gccaccatgc
    47941 cggttacgct gcggtcaccg atggtcacca ggaaagtttt ctccgccacg gtcggcagat
    48001 gcagtacgcg tttcaccgcg tctgcaatgg tgatcccttc acggaccagc gcatcgccct
    48061 tcgctttcag cgtttgtaca tcgcgggtca tcttcggcgt tttaccaagc aggacgtcca
    48121 gcggcagatc gatcggctga ttatcaaaat gacgatcgtg cagagaaaga tgcagttctt
    48181 cggtcgcttc accaatcacc gcgtagggtg cgcgctcacg cttacacagt tcgtcaaaca
    48241 gcggtaattg atcggcagca accgccagca cgtagcgttc ctgggattcg ttacaccaga
    48301 tttccagcgg gctcatgccc ggttcgtcgc ttagaatatc gcgcagttca aatttaccgc
    48361 cgcgcccgcc gtcgctcacc agttccggca tcgcgttaga aagaccgcca gcgccgacgt
    48421 cgtggataaa caggattggg ttggcatcac caagctgcca gcaacggtcg atcacttcct
    48481 ggcagcgacg ctccatctcc gggttgtcgc gctgtacgga agcaaagtcg aggtcggcat
    48541 cagactgacc tgacgccatg gaagacgccg ctccgccgcc aagaccgatg ttcattgccg
    48601 ggccgccgag aacgaccagt ttcgcaccga cgttgatctc gcctttttgt acgtgatcgg
    48661 cgcgaatgtt gccgatcccg cccgccagca tgatcggttt gtgataaccg cgcagctctt
    48721 cgccgttgtg gctgttcact ttttcttcat aagtacggaa gtagccattc agtgccggac
    48781 gaccaaattc gttgttaaac gccgcgccgc ccagcgggcc ttcggtcatg atgtccagcg
    48841 cggtgacaat gcgctcaggc ttaccgaaat cttcttccca cggctgttcg aagccaggaa
    48901 ttcgcaggtt ggaaacggag aaaccaacca aacccgcttt cggcttcgcg ccgcgcccgg
    48961 tggcaccttc atcgcggatt tcaccgccgg aaccggtcgc cgcgcccggc cacggagaaa
    49021 tcgccgtcgg gtggttgtga gtttcgactt tcatcagaat atgcgccggt tcctgatgga
    49081 aatcgtagcg gcccgtttcg tggtcagcaa agtagcggcc cacttcagaa ccttccatta
    49141 cggcggcgtt atctttataa gcagagagaa cgtgatctgg cgtggtttcg aaagtatttt
    49201 tgatcatctt gaacagcgat ttcggctgct gttcaccgtc gataatccag tcggcattaa
    49261 aaattttgtg gcggcagtgc tcagagttcg cctgagcgaa catatacagc tcgatgtcgt
    49321 tcgggttacg accaagcttt gtgaaagcat cctgcagata gtcaatttca tcttccgcca
    49381 gagccaggcc aagacgcagg ttagcgtcga tcagcgcctg acggccctgc cccagcaaat
    49441 caacgctggt aaccggagtc ggttgatggt gggcaaacaa ctgctctgca tcatctaaag
    49501 caaaaaagac agtttccatc atgcggtcgt gcagttcagc ggtaacctgc tgccattgtt
    49561 cattggtcag cgtaccggct tctatatagt aagcaacgcc gcgctcaagg cggtttacct
    49621 gttgtagccc gcagttatga gcaatatcgg tcgctttcga agaccaggga gagatggtgc
    49681 caggacgcgg ggtcaccagc aggagtttgc cttgcggggc gtggctggcg agtgccgggc
    49741 catatttcag caggcgttca agttgtgcgt gctcatcatc gtttaacggc gcattgaggt
    49801 cagcaaaatg gacatactcg gcgtaaatat tgtgaaccgg gagcctggca gcctgaaaac
    49861 gtgccagcag tttgttgatt cggaatgccg acagtgcagg cgaaccacgc agaatttcca
    49921 tcataagtct ctcgtcttct aagctttcgg tgtacccaag gggggaaacg ggcgtcatta
    49981 taaagaatct gatgcgctga cgaaaccgtt tgcgtggaaa taaaatcacc atcgtgaatt
    50041 agcaacgcgt gccgccaatg gctgtaataa gttgccatct ggcgcaggtt tacgcaaaat
    50101 gccgctcatt tatgagtaaa cctttcacta ttattacatt ttttcaagct gggacgcgca
    50161 cgacacagag aattaactaa ttgaaaaaat taaagattaa ttatctgttc atcggcattc
    50221 tggcactgct gctcgcggtc gctctctggc catccattcc ctggtttggt aaagccgaca
    50281 accgtatcgc cgccattcaa gcgcggggag agttgcgtgt gagcaccatt catactcccc
    50341 tgacgtataa cgaaatcaac gggaaacctt ttggcctgga ttacgaactg gcgaaacagt
    50401 ttgccgatta cctcggcgta aaactgaaag tgaccgtgcg gcagaatatc agccagctgt
    50461 ttgacgacct cgataatggt aacgccgacc tgctggcggc aggacttgtc tataacagtg
    50521 agcgggtaaa aaattatcag cctggcccta cctattattc cgtgtcacaa caactggttt
    50581 ataaagtggg tcagtatcgc ccacgtacgc tgggcaacct gacggcggag caactcaccg
    50641 ttgcaccggg tcatgtggtg gttaacgatc tccagaccct gaaagaaaca aaattcccgg
    50701 aattaagctg gaaggtagac gacaaaaaag gctctgcgga attaatggaa gatgtcatcg
    50761 aaggaaaact cgattacacc attgctgatt ctgtcgccat cagcctgttt cagcgcgttc
    50821 acccggagct cgccgtagcg ctcgatatca ccgatgaaca accggtgacc tggtttagcc
    50881 cgttagatgg cgataatacc ctttccgccg ccctgctcga cttcttcaac gaaatgaatg
    50941 aagacggtac gctggcacgc attgaagaga aatacctggg gcatggcgat gattttgatt
    51001 acgtcgatac gcgcacattt ttacgcgccg tcgatgcggt actgccgcag ttaaagcccc
    51061 tgtttgagaa atacgccgaa gaaattgact ggcgtttgct ggccgctatt gcttatcagg
    51121 aatcgcactg ggatgcacag gccacttcac cgacgggtgt gcgcggcatg atgatgttaa
    51181 ccaaaaatac cgcgcaaagc ctcggcatta cggatcgtac cgatgccgaa cagagcatca
    51241 gcggtggcgt gcgttatttg caggatatga tgagtaaagt gccggaaagt gtgccggaga
    51301 acgagcggat ctggtttgcc ctcgctgcgt acaatatggg ctatgcgcat atgcaggatg
    51361 cccgcgccct gacggcaaaa accaaaggga atcctgacag ttgggctgac gtaaaacagc
    51421 gtctgccttt acttagccag aaaccctatt acagcaagct gacttacggc tacgctcgtg
    51481 ggcatgaagc ctacgcttat gtcgaaaata ttcgtaagta tcagattagc ctggtgggtt
    51541 atctgcaaga gaaagagaag caggctacag aagcggcgat gcaactggcg caggattatc
    51601 cggcggtatc gcctacggag ttgggcaaag agaaatttcc ttttctctcg tttctttccc
    51661 agtcgtcatc aaactatttg acccattctc cctctctgct gttttccagg aaagggagtg
    51721 aagagaaaca aaattaatcc gtcgaggatt gcgctttttt ctgcgcttta atttcctggc
    51781 ggcgcatgcg aaagaagtca ctgagcaacg ccgcgcactc atccgccagt attccttccg
    51841 taatttccac tcggtgattc atacccggat gatgcagcac atccattaaa gatcccgcag
    51901 cgccagtttt cgcgtcacgc gcaccaaaga ccacgcgacc aatgcgactg tggatcatcg
    51961 ctccggcaca cattacacat ggttcaagcg tgacatacaa cgtggcgtcg atcagacgat
    52021 aattttgcat caccagaccg ccctgcctca gggccatgat ttctgcatgt gcggtgggat
    52081 catgtcgacc aatcggacgg ttccagcctt cgccaataac acgattgtta tgcactaata
    52141 ccgcgccgac cggtacttcc cgctcatccc aggcacgctt cgccagcgtc attgcatgac
    52201 gcatccagta ttcgtggcta aattcgactt cagacaaaaa gaaaactccg gttataaaag
    52261 cgcggcgcat tatacacgga cgctatgctt tactctattc cagttgctgg agttcaccgc
    52321 gcggggttac tcgccagcga tgctggcaaa agtaaagcag cgggttgtcc tgattactgt
    52381 cgctatagcc actgtacagc cgcagcggcg tgccgatttt gcgctccagt tgcgcgacct
    52441 tttcatgccc gaggcaacgc atcgtcaata cccaaccacc atagccacgc tgaatttggc
    52501 tggcgataag attcacccgc ggcagccagg gtgtatcgaa atagaccgct tcaaccagcg
    52561 gctgtggaga accggtaatc aaccagatat cagcatcgga acttaacagg taggtggtta
    52621 atcgctcctg aaccagcgga aaggcggtaa cattgtcacg aaaccagcgt acgaaatcgg
    52681 cctgcaacgc ctgtaaacgt gcttcgctgt gaccaaaggt gcatccccac agaagcagac
    52741 tcatcggcca gcgtgccgca cgacctttta tcaataacgc aatggctata atcggcaaca
    52801 acggcaggac aagtaacgca ttcagcggtt ggcgacgcag taaatagcgc agaaaactgc
    52861 cgaacatatc ctgctgatgc aatgttccat ctaagtcaaa aaacaccaca cgacgctcgt
    52921 gagttgccaa accatactcc tctggactga aacatcctga ttaattttcg ctactacata
    52981 gcctaacaga tagatcatca cttttccggc aacaatcgac cgcatcatgg ctaactggaa
    53041 tttttaattc atggcaatta gcggcaatgg aatataaaat tcactcgcgt gtgtctcata
    53101 ttgcctgttg tcgccactat ttcatcgcga ggaaagatat gaacggctta cttcgtatcc
    53161 gtcagcgtta ccaggggctt gcccagagtg ataaaaaact ggcggattat ctgctgctac
    53221 aacctgatac ggcgcgccat ttaagctctc agcaactggc caacgaagcc ggagtcagtc
    53281 agtccagcgt cgtgaagttc gcgcaaaaac tcggctataa aggttttccg gcgcttaagt
    53341 tggcgttgag tgaagcgctg gcaagccagc cggaatcgcc ctcagtgccc attcataacc
    53401 aaatccgcgg tgatgatccg ttacggctgg tcggcgaaaa actgattaaa gaaaatactg
    53461 ccgctatgta cgcaacgcta aacgttaata gtgaagagaa actgcatgaa tgcgtaacaa
    53521 tgttgcgctc tgcgcggcgg ataattctga ccggtattgg cgcttcgggt ctggtggcgc
    53581 aaaactttgc ctggaagctg atgaagattg gcttcaatgc tgccgcagtg cgcgatatgc
    53641 atgcgctact cgctaccgta caggcgtcgt cccctgacga tctgttatta gccatttctt
    53701 acaccggtgt acgacgcgag ttaaacctgg cggcagatga gatgctgcga gtgggcggaa
    53761 aagtgctggc gattaccggc tttactccca atgccctgca acagcgtgct tctcattgcc
    53821 tgtataccat tgccgaagaa caagcgacaa acagtgcttc aatctctgct tgtcacgcgc
    53881 agggaatgtt aacggatttg ctgttcattg cgctgattca gcaggatctg gaactggcac
    53941 cagaacgtat tcgtcatagt gaagcgctgg tgaaaaaact ggtctgagta aagaatgcgc
    54001 gtataatgcc cgcccggttt gtgttgtttt gagagtttcc ttatggcgct gttaatcact
    54061 aaaaaatgca tcaattgtga tatgtgtgaa cccgaatgcc cgaatgaggc gatttcaatg
    54121 ggagatcata tctatgagat taacagcgat aagtgtaccg aatgcgtagg gcactacgag
    54181 acaccaacct gccagaaggt gtgcccgatc cccaatacta ttgtgaaaga tccgcaacat
    54241 gtcgagacag aagaacagtt gtgggataaa tttgtgctga tgcaccacgc ggataaaatt
    54301 taatattcta ctctggaagt agagtattaa ttattttact gggaagcctt aacgccatta
    54361 tatttattta attgatgaca ttagcataat aattcactaa gttaatttat atagtatctg
    54421 cccagacact tatttatagt tattaaaggc gcgtccgatt ggttcaccgg acgcacctta
    54481 agtacgtttc cttgtgttat aagaacagaa ggatcagctg taaaacagca atgatgattt
    54541 tgatgacccg tttaatcagg tatcggcaat cagtcattcg tttttcctta aacaaggaat
    54601 gcagccgttc agattagccc ttacatctcc ccaaaactga acgtgcgagt tattggaatc
    54661 gagtgctgca ctccacacca gagctttgac gacaccactc gtttcaatgg gggaattcag
    54721 tggcatggtg taaagcacag caaaattgcc aataacgaaa ccgattttaa tgtacttatc
    54781 aaaaagcggt cacgttaatc ttttcatcaa tgtcgatttc ttcgtaaagg ctcgcgtttt
    54841 ttatgtcaga gattgcaagt tgcttgtaca ggtcaacgaa attgatccat aatcattttc
    54901 gttggaatcg tgtgctgcac aaaattaaag ttaaaaagta aaacccccgt tccttaccag
    54961 ttcgggggtt ttactttata aagagaacgg tattattttt aactttcaat aattaccgtg
    55021 gcacaagcat agtggcgctc atcggccagc gtcacatgca tatttgcaac gcccagcttt
    55081 tccgccagtt ttaatgcctc gccccacagt cgcagccgtg gcttaccaag ctcatcgtta
    55141 aatacttcaa actgattaaa cgccagacca ttgcggatcc cggtgccaaa cgcttttgct
    55201 gcggcttctt ttacggcaaa gcgtttcgcc agaaaacgca ccggctggtg gtgcgttttc
    55261 caaatttccc attcgttatc gcttaatacg cggcgtgcca ggcgctcacc ggatcgggcg
    55321 atcactgctt ctatgcgagc gatctctacg atatccgtgc ctaaacctaa tattgccatt
    55381 agccacgcgc ttccagcatc aggcgcttca tttctgccac cgcatctttc agtccggtca
    55441 tcactgcacg accaataatg gcatgaccga tattcagttc gtgcatctca gggatggcgg
    55501 caatggcttt tacgttgtga taggtcagac cgtgtccggc gttaactttc aaaccgagac
    55561 tagcggcaaa ggtcgcggct ttggcgatac gcgccagctc ttgcgcctgt tcggcgtcag
    55621 ttttggcatc agcatagcaa ccggtgtgga tctcgataaa cggtgcgcca acctctgccg
    55681 cagctttgat ctgctcttca tcggcgtcga taaacagaga aacctgaatc ccggcatccg
    55741 ccagacgttt gcaggcatcg cgcattttgt cacgctgccc tgcaacgtcc aggccgcctt
    55801 cggttgttac ttcctgacgc ttttctggta ccaggcagca aaaatgcggc ttcgtctcaa
    55861 ccgcgatcgc cagcatctct tcggtcaccg ccatctccag attcatgcgg gtatccagcg
    55921 tctgacgcag gatgcgcaca tcgcggtcag taatgtggcg gcgatcttca cgtaaatgca
    55981 cggtaatgcc gtccgccccc gcctgctcgg caataaacgc ggcctgcacc ggatccgggt
    56041 aagcggtacc gcgcgcgttg cgcagcgtag cgatatggtc aatgttgacg cctaacagta
    56101 attcagccat gacaatcctc atcattcata atgtgttttc accgttcgct taggcataaa
    56161 ttgccggaac agttccctgc tctttaaagg tttaccgcca agatacggct taagcgccat
    56221 gcgggtaaag cgtttcgcgg cgcgcagtgt gtctgcgtca ggaaattccc gtgcgtttaa
    56281 cgcttttaac tgccttccgg tgaacgtttt attgtcgata acgacgcttg cgataaaccc
    56341 tttttcttcg cgataacgat acgtcatggt gtcatctacc ggctcgccgc tacccgcaca
    56401 atgggtaaaa ttgacgccat aacccagatg cccgagcagt gccagttcaa agcggcgcag
    56461 cgcgggttct ggcgtaccag tgacccctgc aagagactga atgcagtgca agtaatcgaa
    56521 aaagagttca gagaagcgcg tctcgtattc cagtacgcgg gagagaagtt cgttgatgta
    56581 cagaccgctg taaagcgtga taccgcttaa tggcagcgcc agcgagacag cttcagcact
    56641 gcgcagcgtt ttgacttcgc cacgcccgcc aaaacgtagc aagagagggg tgaaaggctg
    56701 taatgcacct ttcagggtag agcgtttaga gcgtgcgcct ttggcaacca gacgcacgcg
    56761 acccgattcc tccgtgaaga cgtccagcat caggctggtt tcgctccacg ggcgactatg
    56821 caggacaaat gcgcgctgcc agccttccat cggagttact cttaaagatc gtcaacgtaa
    56881 ccgagactgc gcagtgcgcg ttcgtcgtcg gcccaaccgg atttcacttt tacccacagc
    56941 tcaaggtgaa caggcgcttc aaacatttcc tgcatgtctt tacgcgcttc aatcccgatg
    57001 gttttgatct tggccccttt gttgccaatg accatcttct tctgcccttc acgctcaacg
    57061 agaatcaaac cgttgatgtc ataaccaccg cgttcgttag agacgaaacg ttcgatctcc
    57121 acggtcacgg agtacggcag ttcagcgccg aggaaacgca tcagtttttc gcggatgatt
    57181 tcagacgcca taaaacgctg tgagcgatcg gtgatgtaat cttccgggaa gtgatgagtc
    57241 gcttcaggta gatgcttacg cacgattgcc gcaatagtgg caacattcag cccggtttcg
    57301 gcagagattg gcacgatatc gaggaagttc atctggcttg ccaggaactg caggtgcggc
    57361 agcagatcgg ctttctcctg cacgttgtcc actttgttca ccgcgaggat taccggcgct
    57421 ttgccttcgc gcagtttgtt gagcaccatt tcgtcgtccg gcgtccagcg ggtgccttca
    57481 acgacaaaaa tcaccagctc aacatcgcca atagagctgc tcgccgcttt gttcatcagg
    57541 cggttaatag cgcgtttttc ttccatatgc aggcccggtg tatcgacgta gatcgcctga
    57601 tacgcgcctt cagtatggat ccccacaatg cggtgacgag ttgtctgcgc cttgcgggag
    57661 gtgatggaga ttttctgccc cagcagtttg ttcaacaatg tggatttgcc aacattcgga
    57721 cgtccgacga tggcaataaa tccgcagtaa cttttatcga tgctcattcc agctccagtt
    57781 ttttcaacgc ctgttcggcg gcagcctgct cagccttacg acggcttgaa cctgtgccaa
    57841 ccaccggttc actcaggccg ctgacctggc agtggattgt aaattcctga tcgtgcgctt
    57901 cgccacggac ctgaactacc agataagtcg gcagcggcag atgacgaccc tgcaaatatt
    57961 cttgcaagcg cgttttcgga tctttttgtt tatcgcctgg gctaatttcg tccagacgag
    58021 tttgatacca gttgaggatt aatttctcga cggtttgaat atcactgtcg aggaatacgc
    58081 caccaattaa tgcttcgacg gtgtcggcga gaattgactc acgacgaaat ccaccgcttt
    58141 taagttcacc tggtcctaaa cgtaagcact cgcctaactc aaattcgcgc gccagttccg
    58201 ccagcgtatt gccacggacc agcgtggcgc gcatccggct catatcgcct tcatccacac
    58261 gagggaaacg gtgataaagc gcattggcga taacgtagct cagaatagag tcgcctaaaa
    58321 attctaaacg ctcgttatgt ttgctgctgg cactacgatg agttaatgcc tgctgcaaca
    58381 gttcctgatg attaaaagtg tagcccagct tccgttgaag ccgattaatt acgatggggt
    58441 tcatgcgata ccaataaata aatgcgtcaa caattcagca cacgaaacag acctgatata
    58501 catggctctg ctaactgctt cgctgcagtt tcagtttagc ggtatatggg gaccaacgct
    58561 gtttcgtgtg ccgtggcaac ctggaggtgc caaccttaaa cttcggggga atattctata
    58621 cacaacgacg ggggatgtcg ttagccacgg gagatttatc tcataaataa ttcacgttgt
    58681 cgccataacg gcgacaacgt gaacgaagat gagctattaa tggatgccgc caatgcgact
    58741 taagcgcaca cctgtcggcc attcgccttc ttgcttatcg aagctcatcc agatagccgt
    58801 tgcccgaccg accagattcg cttcaggcac aaagccccag taacggctgt ccgcgctgtt
    58861 gtcgcggttg tcgcccatca tgaagtattg tcccggagga acaatccagg ttgccagttg
    58921 ttgccctggc tgctggtaat acatccccac ctgatcctgc gcaatcggca ctgtcagaat
    58981 gcggtgcgtc acatcaccca gtgtctcttt acgctcggaa agacgaattc cattttcttt
    59041 ggtttcgttt ttcggcactt caaagaatcc gctggtcgct tccccaccat tacggcgtga
    59101 gaaggtctga acgaaatcgc tcggttccac gtttgagtag gtgaccggca gcgcgttttc
    59161 acacgcctgg ccggaactgc atcccggttg aatcgtcagc tcttttgaga ccggatcgta
    59221 agtgacttta tcgcccggta aacccaccgc gcgcttgata taatcaagct ttggatcttc
    59281 cggatattta aagaccacga tatcgccgcg tttcggatga ccggtttcga tcagcgtttt
    59341 ctggtagata ggatctttaa tgccataagc aaacttctct accagaataa aatcaccgat
    59401 taacagagtc ggcatcatcg aacctgacgg aatctggaac ggttcataaa taaacgaacg
    59461 cacaatcaat acgatagcca gtaccggaaa aacagaagca ccggtttcca gccagccagg
    59521 cttcggcgca acctttttca acgttgcttt atccagtgag tcaccggcag ccgcctgcgc
    59581 cgctgcctga cgttcccgcc gtttaggtgc gaaaaagaat ttatccacgc accataaaat
    59641 gcccgtcacc agtgtggcaa tcaccagaat cagggcaaac atattcgcca tgccaactcc
    59701 taagggttat ttgttgtctt tgccgacgtg cagaatggcg aggaacgctt cctgcggcag
    59761 ctcgacgtta ccgatctgct tcatgcgttt cttaccttct ttctgcttct gcagcagctt
    59821 tttcttacgg ctgatatcgc cgccataaca tttagccagt acgtttttac gcagctgttt
    59881 cacggtggat cgcgcaataa tgtgcgtacc aatcgctgcc tgaatggcga tatcaaactg
    59941 ctggcgtggg atcagatctt tcatcttctc caccaactcg cgaccgcggt tttgcgaatt
    60001 atcacggtgg gtgatcaacg ccagcgcatc aacacgttca ccgttgatta atacgtctac
    60061 acgtaccatg tcggacgcct ggaagcgttt gaagttataa tccagagacg cataaccacg
    60121 cgaggtagat ttcaggcgat cgaagaagtc gagcaccact tccgccatcg ggatctcgta
    60181 cgtcagcgcc acctgattac cgtggtaaac catattggtc tgcacgccgc gtttttctac
    60241 gcacagcgta ataacgttgc cgagatatgc ctgcggcagc agcatgtgac actctgcaat
    60301 cggctcgcgc agttcgtaaa tgttatttac cgcaggcagc ttggatgggc tgtcgacgta
    60361 gataacttcc cttgacgtgg tttcaacttc atacactacg gtcggcgcag tggtgatcag
    60421 atccagatcg tattcacgtt ccagacgctc ctggatgatc tccatgtgca gcaggccaag
    60481 gaagccgcag cggaaaccaa agcccagcgc gctggagctt tccggctcat agaacagtga
    60541 ggcatcgttc aggctgagtt tacccagcgc gtcacggaag gcttcatagt cgtcggaact
    60601 taccgggaac agaccggcgt atacctgcgg tttgactttc ttaaagccag gcagcgcctt
    60661 ttctgccgga ttacgcgcca gcgttaaggt atcgccgact ggagcgccgt ggatatcttt
    60721 aatcgcacat acgagccagc ctacttcgcc acatttcagt tcagtgcggt caacctgttt
    60781 cggcgtgaag atgcccagac ggtcggcgtt ataggtctgc ccggtactca tgactttcac
    60841 tttgtcgccc ttacgcaggg tgccgttttt aatacggata agtgaaacaa cgcccaggta
    60901 gttgtcgaac catgagtcga taattagtgc ctgcaacggg ccttccggat cgccttccgg
    60961 cggcggaatg tcgcgcacca gacgttcgag aacgtcctgc acgccaacgc cggttttcgc
    61021 tgaacagcgc accgcgtcag tggcgtcgat gccgacgata tcttcaattt cttccgccac
    61081 gcgttcagga tcggctgccg gcaggtcaat cttgttcagt accggcacca cttcgagatc
    61141 catttccatg gcggtgtagc agtttgccag ggtttgcgct tccacgccct gcccggcgtc
    61201 gaccaccagc aatgcacctt cacaggcagc cagcgaacgg gaaacttcat aggagaagtc
    61261 tacgtggccc ggggtgtcga taaagttaag ctgataggtt tcgccgtcag acgctttgta
    61321 gtccagcgtc acgctttgcg ctttgatggt aatgccacgc tcacgctcaa gatccatgga
    61381 atcgagaacc tgcgcctcca tttcacggtc agacaggcca ccgcagatct ggataatacg
    61441 gtcagacagc gtcgatttac cgtggtcaat gtgagctatg atcgaaaagt tacgtatatt
    61501 cttcataaag tatgattatt gtgccttaat gcccggttaa ccaggctttt agaagtcgct
    61561 gttctgagct taacgtctgt attaatagaa acgccgcatt ctacactaca acattgaggc
    61621 gaggaaatgt tcataccgta tggattgtgg tatctggaaa cgtcctcgca tttgttatgc
    61681 aaaatgcaac aaagccagtg aaatcactgg ctcgcgtctt ccgaagatgt ttcaaatcgc
    61741 acaaggccag gcggcaatgc cacgcttaag atgatcggtt gccattctgc ccgggcagca
    61801 aacttacgcg agtagccacg cgcaatcagg aacccgccaa taccgccgag gaccgcacca
    61861 cataatgctg cgacgtcaga agcaaagagt agctgaaata gcgaagcgat gaggaataat
    61921 cccaccagcg gcgacatata aaccagtaat gcggagctaa gcaggctgcc ttcggcgatc
    61981 cccaattcca ctttttgccc cggcactaac ggctcatcac agggtacgac aatggtatgc
    62041 gtggtttgcg ggccaagttt attcaacacg cggctaccgc aaccggcgcg tgaagcgcag
    62101 ctgctgcatg aggctttaac atcacaactg accagcgcct gcccgttttg ccaggagacg
    62161 acggtagccc actctttgat cattgcgctg ccccgaactt aatattctcg gcaatgcgtt
    62221 tcgccgtttg cggcggcagt tcaccgacaa tggtgatttc ggcgttatca cgtacgcttg
    62281 tactgacggt tctgcgtccg gtgcgcaaca tctgatcggt gctcgatggc gtagcgcggt
    62341 taacgtttac cgagaagctg aataatccgt cggaatagag acgtgattcg ataggcatgt
    62401 tgtccatcgt cggtagcgga cgtcgactac tggaaacttc gctaaaaccc tgtggcaacc
    62461 aggttggcgt ccagctgaat ttagcttttt cacctacagg aacagaaagc aacggcggca
    62521 aatttgcctt cgccagcgtc tgcatactgc tgctgatatc ctgattgacg ttaaaagcaa
    62581 tcacgcgaaa ttgttccagc gtttcaccat cgcgatcaag aagatcaacc cgcatcggta
    62641 atttcgattc ggtgtccatc cacacgatgt agctgtagcg tgtaccatct cgggcaacca
    62701 cgcgaatgac ttcgcaaaga cgatcagcaa tacgcgtgcg cccgacggag ataaagtcgt
    62761 agtaaggaga aaggcgtttg aaatcggtat agatgagcga tggcagagaa tcaacaatgt
    62821 aatcgccatt aagcgtgaac ggttcaagtc ccggttcaaa atagctgatt tcattgccgc
    62881 gctgtaccac ttcccggcgc gggccatcca tttgcaacaa ttgtgcaaga ggacggttat
    62941 cgaggcgtgc atgtcgataa cgcagagact caacaccctg tttattgatg ctgatgaatg
    63001 acagctcgta attcagtgac tgactggcca ggttcatctg ctgtaataac gccccggacg
    63061 cgggagtggc cgaggcgtta gcagagaata acaggctacc tgtcactaat gacatggcaa
    63121 accaaagttg cttcattact gcgattgcgt tcctaaagtt tgaattcctg gcacctgtac
    63181 agcggcttgc tgggtttgcg cctgctcaaa ctgaagctgt tcagagtgga gtcggcgttg
    63241 cagttcgtaa tcctgcaaca ttgcattaat gcgacgacgc tgctgctgtt gaccattatt
    63301 tgcggtcgct tcagaaggta ctcccaggct aaccgggctg gctttaccca tcatcggcag
    63361 tgtattaaat accggcgttt cgggctgctg ggacgtttca gattgtccat tatagtgctg
    63421 gacgccaacg ataactgcaa gcgatacgca tgcggctacg cccatttggg taagctgtgc
    63481 cgcccacgga cgtactttct gccagaatgg cattttctgc cattcatgcg gcgcaggctg
    63541 ggcttccggg atcaatgtcg ccggttgacg tactggctct tcttcaatgg cggccatcac
    63601 gcgtgaagag atatcgaaat ggagcacctc gggagtatca ccccgcattg agtcacggat
    63661 taagtgatag ctttcccagg ttttctgcat ttctgggtta tgagccagtt cgttaagcag
    63721 ctcactatcc agcgtttcgc catccattaa agcggaaagt tgttctttct gcatgcctaa
    63781 tacccttatc cagtatcccg ctatcgtcaa cgcctgataa gcggttgaac tttgttatca
    63841 atagcttccc tcgctcggaa gatacgtgaa cgcaccgtac ctaccggaca atccatgata
    63901 gcggctatct cttcatagct caggccatcc agctcccgca aggttattgc catgcgtaaa
    63961 tcttccggga gggactcaat agttcggaaa actatctgtc tcagttcttc tgacaacatt
    64021 aagttctcag ggttcgaaat ttctttcaac gcgccgccac tttcgaagtt ttcagcttca
    64081 atggcatcca catcactgga aggtggacga cgcccctgag caaccaggta atttttcgct
    64141 gtatttacag caatccgata cagccatgta taaaaagcgc tatctccccg gaacgaatcc
    64201 agcgcacgat aggctttaat aaaagcttct tgtaccacat cgggaacatc acccgacggc
    64261 acatagcggg aaaccagact cgccacttta tgctgatagc gcactaccag taagttaaag
    64321 gctttctgat ctcccttctg gacccgttca accaggacct ggtccgttaa ctgctcgctc
    64381 atccgaggta aagtctcccc aaaccaaatt tccacgcgct atcgaaacgc cactccatta
    64441 gctgcaattt gagcaagcaa agggttagag tgtctcgttt ttgtaaagtt ccgtaacgca
    64501 tctgtttttg tttgtcatgc tgtagacgga tcattatcta tcattataag tctacagaat
    64561 ctgaacatcg cattatctgt gtagaaatgc ccatttaact gcctgaagag taacccaacg
    64621 gcctttttat ttcaccacct aatcctccac cagccagtaa cttctctttt tctcgccgcc
    64681 ctgcgtcagc gtgtttagtc actgtaacaa atattaaaat agcaggtgtt tattcgcaca
    64741 acatgatgct atgctgacca aaccgtgttt agtaaattaa acaaagaaaa tgaatactct
    64801 ccttgaacat tcatgtgacg tgttgattat cggtagcggc gcagccgggc tttcactggc
    64861 gctacgcctg gctgaccagc atcaggtcat cgttctaagt aaaggcccgg taacggaagg
    64921 ttcaacattt tatgcccagg gcggtattgc cgccgtgttt gatgaaactg acagcattga
    64981 ctcgcatgtg gaagacacat tgattgccgg ggctggtatt tgcgatcgcc atgcagttga
    65041 atttgtcgcc agcaatgcac gatcctgtgt gcaatggcta atcgaccagg gggtgttatt
    65101 tgatacccac attcagccga atggcgaaga aagctaccat ctgacccgtg aaggtggaca
    65161 tagtcaccgt cgtattcttc atgccgccga cgcaactggt agagaagtag aaaccacgct
    65221 ggtgagcaag gcgctgaacc atccgaatat tcgcgtgctg gagcgcagca acgcggttga
    65281 tctgattatt tctgacaaaa ttggcctgcc gggcacgcga cgggttgttg gcgcgtgggt
    65341 atggaaccga aataaagaaa aggtggaaac ctgccacgca aaagcggtgg tgctggcaac
    65401 cggcggtgcg tcgaaggttt atcagtacac caccaatccg gatatttctt ctggcgatgg
    65461 cattgctatg gcgtggcgcg caggctgccg ggttgccaat ctcgaattta atcagttcca
    65521 ccctaccgcg ctgtatcacc cacaggcacg caatttcctg ttaacggaag cactgcgcgg
    65581 cgaaggcgct tatctcaagc gcccggatgg cacgcgtttt atgcccgatt ttgatgtgcg
    65641 cggcgaactg gccccgcgcg atattgtcgc ccgcgccatt gaccatgaaa tgaaacgcct
    65701 cggcgcagat tgtatgttcc ttgatatcag ccataagccc gccgatttta ttcgccagca
    65761 ttttccgatg atttatgaaa agctgctcgg gctggggatt gatctcacac aagaaccggt
    65821 accgattgtg cctgctgcac attatacctg cggtggtgta atggttgatg atcatgggcg
    65881 tacggacgtc gagggcttgt atgccattgg cgaggtgagt tataccggct tacacggcgc
    65941 taaccgcatg gcctcgaatt cattgctgga gtgtctggtc tatggctggt cggcggcgga
    66001 agatatcacc agacgtatgc cttatgccca cgacatcagt acgttaccgc cgtgggatga
    66061 aagccgcgtt gagaaccctg acgaacgggt aataattcag cataactggc acgagctacg
    66121 tctgtttatg tgggattacg ttggcattgt gcgcacaacg aagcgcctgg aacgcgccct
    66181 gcggcggata accatgctcc aacaagaaat agacgaatat tacgcccatt tccgcgtctc
    66241 aaataatttg ctggagctgc gtaatctggt acaggttgcc gagttgattg ttcgctgtgc
    66301 aatgatgcgt aaagagagtc gggggttgca tttcacgctg gattatccgg aactgctcac
    66361 ccattccggt ccgtcgatcc tttctcccgg caatcattac ataaacagat aaaaagcctg
    66421 ggttagcgcc gtatacgctt cggaatagtt ctggtctggc ccacgaatga ctaagcgatc
    66481 gctaaagcat tctcccgcct gcggggagaa tgccagcagc acccgatgcg gcagtcgcgc
    66541 ttcgttttcc gccacatccg tccgcaaacg taaatgccag cccatactta atgccagctc
    66601 cgtaaaacta ttaccaatct gctctggcag caccacgcag aaaaatccct cttcggtaat
    66661 gcactccgcc gcgcaagtaa gtaaagtctg gtgatcaagt gaggtggtgt agcgcgcctg
    66721 ttcccgctgc ggcgtcgcgc actccactcc ttgctcataa tacggcgggt tgctgatgat
    66781 taaatcaaag cgcgcggtct gctgtgttac ccactgctga atatccgccg tatggacgtt
    66841 aattctctct aaccacgggg actgggcgac attttcctgc gcctgagtgg cagcttcact
    66901 ttccagctca acggcatcaa ttatcacgct gtcactggtt cgctgcgcca gcatcagtgc
    66961 cagcaagcca ctacccgcac cgatatcaag gcaacgtttt accccagcca ccggtgccca
    67021 tgcgcccagc aatatgccat ccgttcccgc tttcatcgca cagcgatcgt gagcaacaaa
    67081 aaactgttta aaagtaaatc cattacgacg aagcacggat gtagactgtg acatgaaaat
    67141 aaaaccttgc aggaaaaacg gcgatagcac cgggtgagaa caatacccga gaagcgatat
    67201 ccatacaaac agatgaagat tgcagccgta acgtctataa tcagcgcccc acacagaggt
    67261 agaacatgac tgtaacgact ttttccgaac ttgaactcga cgaaagcctg ctggaagccc
    67321 tccaggataa aggtttcact cgcccgaccg ccattcaggc tgccgccatt ccgcctgcgc
    67381 tcgatggccg tgatgtactc ggttctgcgc cgacaggcac cggtaaaacg gcggcgtatc
    67441 tgctgccagc gttgcagcac ctgctcgatt tcccgcgtaa gaaatccggt ccgccgcgta
    67501 ttttgatcct caccccaact cgcgagctgg cgatgcaggt ggccgatcat gcccgcgaac
    67561 tggcgaaaca tacgcatctg gatatcgcca ccatcaccgg cggcgtagcc tatatgaacc
    67621 acgcggaagt gttcagcgaa aatcaggaca tcgtggtcgc cacgaccgga cgtctgctgc
    67681 aatacataaa agaagagaac ttcgattgcc gcgcggttga aacgctgatc ctcgacgaag
    67741 cagaccgtat gctggatatg ggcttcgctc aggatatcga acatattgct ggcgaaacgc
    67801 gctggcgtaa acagaccctg ctcttttcgg caacgctgga aggcgatgcg attcaggact
    67861 ttgccgagcg tctgctggaa gatccggtgg aggtttctgc caatccgtcc acccgtgagc
    67921 gcaaaaaaat tcatcagtgg tattaccgcg ccgatgacct ggaacataaa accgcattgc
    67981 tggtgcatct gttaaaacag ccggaagcga cccgctcaat tgtgtttgtg cgtaagcgtg
    68041 agcgtgtgca tgagctggca aactggctgc gcgaagcggg catcaacaac tgctatctcg
    68101 aaggtgagat ggtacagggc aagcgtaacg aagcgatcaa acgtttgacc gaaggtcgcg
    68161 taaacgtact ggttgcaacc gatgttgccg cgcgcggtat cgacattcct gacgtcagcc
    68221 acgtctttaa cttcgatatg ccgcgcagtg gcgatactta tttgcaccgt atcggacgta
    68281 ccgcgcgcgc cggtcgtaaa ggcaccgcaa tttcgctggt agaagcccat gaccatctgc
    68341 tgctgggcaa agttggtcgc tatattgaag agccaattaa agctcgcgta attgatgagt
    68401 tacgcccgaa aacgcgtgca ccaagcgaga agcagaccgg caagccatcg aagaaagtac
    68461 tggcaaaacg cgccgagaag aaaaaagcaa aagagaaaga gaagccgcgg gtgaaaaaac
    68521 gccatcgcga taccaaaaat attggtaagc gccgtaaacc aagcggaacg ggcgtgccac
    68581 cgcaaacgac agaagagtaa tctcaatgcc gggtttaagc ccggcattat ttttatcccg
    68641 gcaaaaaaca atcttcaaca cactgaataa aagtgtgcat cgccgggcta accgcttttc
    68701 cggcatggtg agcgcacatt gccgtaatgg tctgcgagtg ttcgccaaaa ggcaattcaa
    68761 ttaattctcc gctctcaagc tctttttcca ccgcaaagcg gggcaagtaa ctgacaccga
    68821 tattcgccgc gacacaacgt ttgatacttt caatgcttaa cagctcaatg gtgttttcca
    68881 ccgtgatccg ccgctggcgc agcgtgctct caaatatctg ccggaataca cattgcggtt
    68941 cgttgataat aaagctacag gcgttatgtc ttcccggctc agtaaaatcg acatctgcaa
    69001 tttgcggtga agctaccagc accagcgatt gctcacccaa ttctcgtcgg ttcagggcat
    69061 catcattccc tacgcgataa aaaaccccga catcggcctc atcattcaat agcgcatcac
    69121 ggatcacgta acagttcaga gactgcaacg ataaacgcac ttttggggcg cgttgccgaa
    69181 aacgctgcaa cacctgcggc attcggtaag agagcaacgt ttcgcccgaa acaacgcgta
    69241 gttccccgtc cggatccgac tcctttttgg cagcctcacg aagggtatcc atcactctgg
    69301 tgagttcata aatgtgcggc agcagctttt taccttcgcg agtgaggcac atccgtcggc
    69361 caattttctc aaataactgt accgagaact cctgctcaag ctgctgaata tgaaaagtca
    69421 ccgtcgattg tgtacagcac aatttttgcg aagctcgcaa aaaggaaccc tcttccacca
    69481 cggttttaag cgtaataaaa cggcgcagat ccataacccc aaacctatcg aaaatatcga
    69541 atttagaatg taaaaacatt cattttttta aatattctgt gtcgggtact gtctaccaaa
    69601 acagaggaga taacaagtga caccgaccct tttaagtgct ttttggactt acaccctgat
    69661 taccgccatg acgccaggac cgaacaatat tctcgccctt agctcagcca caacgcacgg
    69721 atttcatcag agtacccgcg tgctggcagg gatgagtctg ggatttttaa tcgtcatgtt
    69781 gctgtgtgcg ggcatttcgt tttcactggc tgttatcgac ccggcagctg tgcatctgtt
    69841 gagttgggcg ggggcggcat atattgtctg gctggcgtgg aaaatcgcta ccagtccaac
    69901 aaaggaagac gggcttcaga caaaaccaat cagtttttgg gccagctttg ctttgcagtt
    69961 tgtgaacgtc aaaatcattt tgtacggtgt tacggcactg tcgacatttg ttctgccgca
    70021 aacacaggcg ttaagctgga tagttggcgt cagcgttttg ctggcaatga ttgggacgtt
    70081 tggtaatgtg tgctgggcgc tggcggggca tctgtttcag cgattgtttc gccagtatgg
    70141 tcgccagtta aatatcgtgc tggcgctgct gctgatctat tgcgcggtac gcattttcta
    70201 ttaatgaaaa aaagcggaag aggtcgccct cttccgctta gtaacatgct acttaagcct
    70261 tacaggcttt cagtaaaggt acgagcgata acgtcgcgct gctgttccgg agtcagagag
    70321 ttaaagcgaa ctgcataacc ggatacacgg atggtcagct gcggatattt ttccggatgc
    70381 ttaactgcat cttccagagt ttcacgacgc agaacgttaa cgttcaggtg ctgaccacct
    70441 tcaacgcgaa cttctggttt cacttctact ggaacttcac ggtactcaat gtcacccagt
    70501 ttgcttactg caaccacttc gtcttctgca taacctgctt tcgcaacgat gcaacgcgct
    70561 tcgccttttt cgctgtccag cagccagaaa gagttcagca gatcgtcgtt agcggcttta
    70621 gtaatctgga tacctgtaat catgtgatgc ctccccggca aaattatttg atttgttcag
    70681 cctgtcgcgg ccaattggta aaaccattgt tgcttgagtg tatatatact cctcaaacac
    70741 ccttgaatct ttgatttaaa tcaataaaaa ccacacatca agtgtggtcg caaatagatt
    70801 ttattgtttt acatcaactt atgcgggtgt gaaattttac caatttacat ttttttgcac
    70861 tcgtttaagt ctaaaaaatg agcatgattt tgttctgtag aaagaagcag ttaagctagg
    70921 cggattgaag attcgcagga gagcgagatg gctaacgaat taacctggca tgacgtgctg
    70981 gctgaagaga agcagcaacc ctattttctt aatacccttc agaccgtcgc cagcgagcgg
    71041 cagtccggcg tcactatcta tccaccacaa aaagatgtct ttaacgcgtt ccgctttacc
    71101 gagttgggtg acgttaaagt ggtgattctc ggccaggatc cttatcacgg accgggacag
    71161 gcgcatgggc tggcattttc cgttcgcccc ggaatcgcca ctcctccgtc actgctgaat
    71221 atgtataaag agctggaaaa tactattccg ggcttcaccc gccctaatca tggttatctt
    71281 gaaagctggg cgcgtcaggg cgttctgcta ctcaatactg tgttgacggt acgcgcaggt
    71341 caggcgcatt cccacgccag cctcggctgg gaaaccttca ccgataaagt gatcagcctg
    71401 attaaccagc atcgcgaagg cgtggtgttt ttgttgtggg gatcgcatgc gcaaaagaaa
    71461 ggggcgatta tagataagca acgccatcat gtactgaaag caccgcatcc gtcgccgctt
    71521 tcggctcatc gtggattctt tggctgcaac cattttgtgc tggcaaatca gtggctggaa
    71581 caacatggcg agacgccgat tgactggatg ccagtattac cggcagagag tgagtaaatt
    71641 tgcgggaaaa tgccggatgg cagagttgcc acccggctga tttatcaggc tttattctga
    71701 cgccaccatt cgccaagaag aacgccggtt gcgacagaga tattcagccc ggcaacgttg
    71761 ccagtaccat caatcttcac gcgcagatcg ttcggatcgc gtgcggcatc cggtaaccct
    71821 tcatattcct gacccagcac cagtaccatt ttcgctggca gactggtttt gaacagcggt
    71881 ttaccctgct cgctggaagt tgtcactacg gtgtaacccg cctgacggaa atcatccagc
    71941 acgttaacaa tgttgtcgcc agtaatcggc tgaacgtgct ctgcgccgcc ttctgcggta
    72001 cggatagccg cccccgactc cagcagtgcc gcatcctgca ccacaacacc tttcacgcca
    72061 aagtgcgcgc agctgcgcat catgccaccc aggttatgcg ggttagattc gttttccagt
    72121 gccagaacac aatcctgcgc gcctgcctgg cttacccact gctgcacggt tgtgccatta
    72181 cgctttttga tcaggaagca aacgccaccg tgatgttccg tgcctgacgc ttttgtcaat
    72241 tccgcttcat ctaccacatg gtacgctttg cggtttgctg ccatccagcg caaggcttct
    72301 ttaaaacgcg gcgttacact ctggataaac caggcgcgaa caatcgcttc cggacggctc
    72361 tggaacagtg cctgacaggc gttttcgccg tagacgcggg tttcttccgc acgctgacga
    72421 cgcaacactt ccggatcaat aaaactttta ccactgatgc caccgtgatc ggccttttcc
    72481 ggcgtctcat cacccggcgc gcgggaaacc gtgcgccacg gcgaatcttc ccacttgcgg
    72541 tcacggggct gactctgttt gtcatcgcgg gcggggcgac ggccaccgtc agcacgagat
    72601 tttcctggac gcccgccccc tttcccggta cgcgggttgt gggtacgttt atcagaatca
    72661 tcatcactgc ggacatacat cactttgacc ttgccgcttt tacctttcat ttcatcgttc
    72721 atgcttttct ccaccagcgc tgcgcgaagc gcgcagatta cccgaagtcc gcgcggttcg
    72781 ccatgatttc gtaccaaagc ctgcgactat catacctatt gaataaaaca gattgttgtc
    72841 tggaacaatg tccccgataa tatgtaacat attagaaaca taccggcgtt gttgccgata
    72901 agtctcctta ctcatcccga ggttagttat gaataccgtt tgtacccatt gtcaggccat
    72961 caatcgcatt cccgacgatc ggatcgaaga tgcggcaaaa tgcggacgct gcggtcacga
    73021 cttgtttgac ggagaggtga ttaatgcgac cggtgaaacg ctcgacaaat tgctgaaaga
    73081 tgatctacca gtggtgatcg acttctgggc accgtggtgc ggcccctgcc gtaatttcgc
    73141 accaattttt gaagatgtcg cgcaagagcg tagcggtaaa gtgcgctttg tgaaagtgaa
    73201 taccgaagct gaacgtgaat tgagcagtcg ctttggaatt cgtagtatac cgacgatcat
    73261 gattttcaaa aacggtcagg ttgtcgacat gcttaatggc gcagtaccga aagcgccgtt
    73321 cgatagctgg ctgaacgaat ctctttaatc ttaccggggc gcatcttgtg ccccgttttc
    73381 tcctctgcga caatggcgtt ttttcgacgc tctcttatga ccgaaaacgc tgttctccag
    73441 ttacgcgccg agcgtattgc gcgcgcaaca cgtccttttc ttgcccgcgg taatcgcgtt
    73501 cgtcgctgcc aacgctgtct tttgccagag aaattatgtc tctgttcgac aattacccca
    73561 gcacaagcaa aaagtcgctt ctgtttgctg atgttcgaca ccgagccaat gaagcccagt
    73621 aataccgggc gtctgattgc tgatattttg cctgataccg ttgcgtttca atggtcgcgt
    73681 accgaaccct cgcaggattt gctggatctg gtacaaaacc cggattatca gccaatggtg
    73741 gtctttcccg cctcgtatgc tgatgagcaa cgggaagtga tcttcacacc acctgccggt
    73801 aagccaccgc tgtttatcat gctcgatggt acctggccgg aagctcgcaa gatgtttcgt
    73861 aaaagtccgt atctggataa tcttcccgtc atttccgtcg atctttcccg actttctgcc
    73921 tatcgcctgc gtgaagccca ggctgaaggc caatattgta ctgccgaggt agccatcgca
    73981 ctgttagata tggccggcga taccggggcg gcagcagggt taggcgagca cttcacccgc
    74041 tttaaaacgc gctatctggc aggaaaaacg caacatctgg gtagcatcac agcagaacag
    74101 ttagaaagcg tttaaaatca ttcggtcact tctgcgggag accggtatga gtcagcgagg
    74161 actggaagca ctactgcgac caaaatcgat agcggtaatt ggcgcgtcga tgaaacccaa
    74221 tcgcgcaggt tacctgatga tgcgtaacct gctggcggga ggctttaacg gaccggtact
    74281 cccggtgacg ccagcctgga aagcggtgtt gggtgtgttg gcctggccgg atattgccag
    74341 cttgcccttt acacccgacc ttgcggtttt atgtaccaat gccagccgta atcttgctct
    74401 tctggaagag ctcggcgaga aaggctgtaa aacctgcatt attctttccg ccccggcatc
    74461 gcaacacgaa gatctccgcg cctgcgccct gcgccataac atgcgcctgc ttggaccaaa
    74521 cagtctgggt ttactggctc cctggcaagg tctgaatgcc agcttttcgc ctgtgccgat
    74581 taaacgcggc aagctggcgt ttatttcaca gtcggctgcc gtctccaaca ccatcctcga
    74641 ctgggcgcaa cagcgtgaga tgggcttttc ctactttatt gcgctcggcg acagcctaga
    74701 tatcgacgtt gatgaattgc ttgactatct ggcacgcgac agtaaaacca gcgccatcct
    74761 gctctatctc gaacagttaa gcgacgcgcg acgctttgtt tcggcggccc gtagtgcctc
    74821 gcgtaataaa ccgattctgg tgattaaaag cggacgtagc ccggcagcac agcgactgct
    74881 caacacgacg gcaggaatgg atccggcgtg ggatgcggcg attcagcgtg ccggtttgtt
    74941 gcgggtgcag gatacccacg agctgttttc ggcggtggaa accctcagcc atatgcgccc
    75001 gctgcgtggc gaccggttga tgattatcag caacggtgct gcgcctgccg cgctggcgct
    75061 ggatgcctta tggtcacgca atggcaagct ggcaacgcta agcgaagaaa cctgccagaa
    75121 actgcgcgat gcactgccag gacatgtggc aatctctaac ccgctcgatc tgcgcgatga
    75181 cgccagcagc gagcattatg tcaaaacgct ggacattctg ctccacagcc aggattttga
    75241 tgcgctgatg gttattcatt cgcccagcgc cgctgctccc gcaacagaaa tcgcgcaagt
    75301 attaattgaa gcggtaaagc atcatccccg cagcaagtat gtttctctgc tgacgaactg
    75361 gtgcggcgag cactcctcgc aagaggcacg acgtttattc agcgaagccg ggctgccgac
    75421 ctaccgtacc ccggaaggaa ccatcactgc ttttatgcat atggtggagt accggcgtaa
    75481 tcagaagcaa ctacgcgaaa cgccggcgtt gcccagcaat ctgacttcca ataccgcaaa
    75541 agcgcatctt ctgttgcaac aggcgattgc cgaaggggct acgtcgctcg atacccatga
    75601 agttcagccc atcctgcaat cgtatgggat gaacacgctc cctacctgga ttgccagcga
    75661 tagcaccgaa gcggtgcata ttgccgaaca gattggttat ccggtggcgc tgaaattgcg
    75721 ttcgccggat attccacata aatcggaagt tcagggcgtc atgctgtacc tgcgtacagc
    75781 caatgaagtc cagcaagcgg caaacgctat tttcgatcgc gtaaaaatga cctggccgca
    75841 ggcgcgggtc cacggcctgt tggtgcaaag tatggctaac cgtgctggcg ctcaggagtt
    75901 gcgggttgtg gttgagcacg atccggtttt tgggccgttg atcatgctgg gtgaaggcgg
    75961 tgtggagtgg cgtcctgaag atcaagccgt cgtcgcactg ccgccgctga atatgaacct
    76021 ggcccgctat ctggttattc aggggatcaa aagtaaaaag attcgtgcgc gcagtgcgct
    76081 acgcccattg gatgttgcag gcttgagcca gcttctggtg caggtttcca acttgattgt
    76141 cgattgcccg gaaattcagc gtctggatat tcatcctttg ctggcttctg gcagtgaatt
    76201 taccgcgctg gatgtcactc tggatatcgc gccgtttgaa ggcgataacg agagtcggct
    76261 ggcagtgcgc ccttatccgc atcagctgga agaatgggta gaattgaaaa acggtgaacg
    76321 ctgcttgttc cgcccgattt tgccagaaga tgagccacaa cttcagcaat tcatttcgcg
    76381 ggtcaccaaa gaagatcttt attaccgcta ctttagcgag atcaacgaat ttacccatga
    76441 agatttagcc aacatgacgc agatcgacta cgatcgggaa atggcgtttg tagcggtacg
    76501 acgaattgat caaacggaag agatcctcgg cgtcacgcgt gcgatctccg accctgataa
    76561 catcgatgcc gaatttgccg tgctggttcg ctcggatctc aaagggttag gcttgggtcg
    76621 acgcttaatg gaaaagttga ttacctatac gcgagatcac ggactacaac gtctgaatgg
    76681 tattacgatg ccaaacaatc gtggcatggt ggcgctggcc cgcaagctcg ggtttaacgt
    76741 tgatatccag ctcgaagagg ggatcgttgg gcttacgcta aatcttgccc aacgcgagga
    76801 atcatgagta aggtactgga aatgttgacc acttaatcgg gactggtgtt attattgcac
    76861 gcttatgtcg tctgcattgc acagaggacc cttcaatgaa cagagaagaa atgcactgtg
    76921 atgttgtcaa aatttaagcg taataaacat caacaacacc ttgcccaact acccaagatt
    76981 tctcaatcag ttgatgatgt cgatttcttt tacgctcccg ccgacttccg ggagacgctg
    77041 ctggaaaaaa tagccagcgc gaagcagcgc atttgcattg tcgccctgta tctcgaacag
    77101 gatgacggtg gcaaaggcat tctgaacgcg ttgtatgaag ctaaaaggca gcgtccggaa
    77161 ctggatgtgc gggtgctgct cgactggcat cgtgcacaac gtggacgcat tggcgctgcg
    77221 gcatctaaca ctaacgctga ctggtactgt cgcatggcgc aggaaaatcc gggcgtagat
    77281 gttccggttt atggcgttcc aatcaatacc cgtgaagccc ttggtgttct gcactttaaa
    77341 ggctttatca tcgacgatag cgtactttat agcggtgcca gcctgaacga tgtttacctg
    77401 catcagcacg ataaatatcg ctacgaccgt tatcatctta tccgtaaccg taagatgtca
    77461 gacattatgt ttgaatgggt tacacagaat attatgaatg gccgcggcgt taatcgtctg
    77521 gatgatgtta atcggccaaa aagcccggaa atcaagaacg atattcgtct gttccgccag
    77581 gagctgcgtg atgccgctta tcatttccag ggcgatgccg acaacgatca gctttccgta
    77641 acgccgctag tggggctggg gaaatcgagt ctgttgaaca agaccatttt ccatcttatg
    77701 ccttgtgccg agcagaaact aaccatctgt acgccatact tcaacctgcc agcaatcctt
    77761 gtgcgcaata ttatccagtt gctgcgcgaa gggaaaaagg tcgaaattat tgttggtgat
    77821 aaaaccgcga acgacttcta cattccggaa gatgaacctt tcaagataat tggcgcattg
    77881 ccttatctct atgagatcaa tctgcgtcgt ttcctgagcc gtttgcagta ttacgtcaat
    77941 actgaccagc tagtggttcg gttatggaaa gatgacgaca acacctatca cctaaaaggg
    78001 atgtgggttg atgataagtg gatgttgatc accggtaata acctgaaccc gcgcgcctgg
    78061 cgtctggatc tggaaaacgc cattttgatc cacgatccac aactggagct tgcgccgcag
    78121 cgagagaaag aactggagct gatccgcgag cataccacca tcgttaagca ctatcgcgat
    78181 ctgcaaagta ttgccgatta tccggtgaag gttcgtaaac tcatccgccg tttgcgccgt
    78241 atccgcatcg accgattaat tagccgcatc ctgtaatcac aaccccgtcc tgtacggggt
    78301 ttgttttttg gaggccacgt tttgcgtatt ctttttgtct gttcgctatt gttactttct
    78361 ggatgcagcc atatggctaa cgatagctgg agcgggcagg ataaagctca acactttatc
    78421 gcctcggcga tgctttccgc cgccggaaat gaatattcac agcatcaggg gatgagccgg
    78481 gatcgcagtg ccatgtttgg tttgatgttc tctgtcagtt tgggggcgtc aaaagagttt
    78541 tgggatagcc gccccgaagg gagcggctgg agctggaagg atttggcctg ggatgtcgcc
    78601 ggtgcaagca ccggctatac cgtctggcaa ctgacccgtc actaaagacg catccccttc
    78661 cctttgcgat gtagcatcaa agaaaccaga aacgccacca cggccatcaa ggtcacatac
    78721 cagaagaagg ctgtttccat tcctattgat ttcagcgaca acgctacgta ctccgccgaa
    78781 ccaccaaata tagcattagc gaccgcatat gacagaccaa cgcctaatgc gcgaacctgt
    78841 gccgggaaca tctcagcctt cagtattcca ctgattgatg tataaaaact cactatcagc
    78901 agggcacaca tcaccagacc aaaagcggca taaggcgagg aaacgttttg caatgctgag
    78961 agaataggaa cggtaaaaat ggctgccagc gaaccgaaac ataacattga ggtacggcga
    79021 ccaatcttat ccgacagcgc gccaatgagt ggttgaataa gcatgaatac aaacaatgcg
    79081 gcagtcataa tgccactcgc cacgttggca tgcattcccg cagtatttac cagatacttc
    79141 tgcatataag tggtgaaggt atagaaacaa agggagcccg cagcggtaaa accgagaacc
    79201 atgatgaatg cacggcgatt gcgccataat cctttcagag atccagcttc ttttaaagcg
    79261 cgcgtttctt gttgcgaagt ttcatctaac tgacgacgta accacaacgc cacaacagct
    79321 aacacagctc ctaacgcgaa aggaatacgc catccccact ctctgagtgc agagtcttcc
    79381 atggtgtgtt gtaaaaccac gacaaccagt agggctagca gttgtccgcc gatcaacgtc
    79441 acatactgaa atgatgcgta aaaacctttg cgcccttcaa cggcaacttc actcatatag
    79501 gtggcgctgg tgccatattc tccgccaacg gataatccct gaaataaacg cgcgagaagc
    79561 aataatgccg gagcccacgt accgattgtt tcataaccag gcaaacaggc gattaccagt
    79621 gagccgaaac acatcataca caccgatagc agcatcgatt ttttgcgacc atgtttatcg
    79681 gctatgcggc caaatagcca accgcctatt gggcgcatca ggaatcccgc agcaaaaaca
    79741 cctgctgttt gtagtagttg agtcgtcgtg ttcccggaag ggaagaagat gtgggcaaag
    79801 tagagtgaac agaacgagta gacatagaaa tcgaaccact cgaccagatt acctgaagag
    79861 gcccccacaa tcgcccaaat gcggcgacga gtatcactac ttgtcagttt gctgtctgcc
    79921 gttacagtac tttcagccat gccattatgt ctcctgccgt aatccgatgc ttttgtcggt
    79981 cgcttttgtt tatttctttt ggcaaagaaa atgttataca tttgttacgt atcattatgc
    80041 aaccttaacc atgaatttag ttagcaggaa aatggttatt gaggagctta aggataaatt
    80101 tctggtaagg aggacacgta tggaagtagg caagttgggg aaaccgtatc cgttgctgaa
    80161 tctggcatat gtgggagtat aagacgcgca gcgtcgcatc aggcattttt ttcaccaacg
    80221 caaaaaggcc atcctgacgg atggccttt
//
LOCUS       DBFIFI010000002        78017 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.002, whole genome shotgun sequence.
ACCESSION   DBFIFI010000002 DBFIFI010000000
VERSION     DBFIFI010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 78017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78017
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.002"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..1097
                     /gene="proV"
                     /locus_tag="N7B39_000074"
     CDS             <1..1097
                     /gene="proV"
                     /locus_tag="N7B39_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HGU2420145.1"
                     /translation="KTGLSLGVKDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEP
                     TRGQVLIDGVDIAKISDAELREVRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINA
                     EERREKALDALRQVGLENYAHSYPNELSGGMRQRVGLARALAINPDILLMDEAFSALD
                     PLIRTEMQDELVKLQAKHQRTIVFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEIIN
                     NPANDYVRTFFRGVDISQVFSAKDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGY
                     VIERGNKFVGAVSIDSLKTALTQQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAV
                     PVVDEDQQYVGIISKGMLLRALDREGVNNG"
     gene            1090..2154
                     /gene="proW"
                     /locus_tag="N7B39_000075"
     CDS             1090..2154
                     /gene="proW"
                     /locus_tag="N7B39_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HGU2420146.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            2211..3203
                     /gene="proX"
                     /locus_tag="N7B39_000076"
     CDS             2211..3203
                     /gene="proX"
                     /locus_tag="N7B39_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HGU2420147.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            3395..4579
                     /locus_tag="N7B39_000077"
     CDS             3395..4579
                     /locus_tag="N7B39_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2420148.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISNKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            4703..5440
                     /gene="ygaZ"
                     /locus_tag="N7B39_000078"
     CDS             4703..5440
                     /gene="ygaZ"
                     /locus_tag="N7B39_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HGU2420149.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            5430..5765
                     /gene="ygaH"
                     /locus_tag="N7B39_000079"
     CDS             5430..5765
                     /gene="ygaH"
                     /locus_tag="N7B39_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HGU2420150.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            5856..6386
                     /gene="emrR"
                     /locus_tag="N7B39_000080"
     CDS             5856..6386
                     /gene="emrR"
                     /locus_tag="N7B39_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HGU2420151.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDHLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            6513..7685
                     /gene="emrA"
                     /locus_tag="N7B39_000081"
     CDS             6513..7685
                     /gene="emrA"
                     /locus_tag="N7B39_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HGU2420152.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQQMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            7702..9240
                     /gene="emrB"
                     /locus_tag="N7B39_000082"
     CDS             7702..9240
                     /gene="emrB"
                     /locus_tag="N7B39_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HGU2420153.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(9304..9819)
                     /gene="luxS"
                     /locus_tag="N7B39_000083"
     CDS             complement(9304..9819)
                     /gene="luxS"
                     /locus_tag="N7B39_000083"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HGU2420154.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     VWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(9969..11525)
                     /gene="gshA"
                     /locus_tag="N7B39_000084"
     CDS             complement(9969..11525)
                     /gene="gshA"
                     /locus_tag="N7B39_000084"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HGU2420155.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIDKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            complement(11598..12026)
                     /gene="yqaA"
                     /locus_tag="N7B39_000085"
     CDS             complement(11598..12026)
                     /gene="yqaA"
                     /locus_tag="N7B39_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HGU2420156.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFCPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(12023..12589)
                     /gene="yqaB"
                     /locus_tag="N7B39_000086"
     CDS             complement(12023..12589)
                     /gene="yqaB"
                     /locus_tag="N7B39_000086"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HGU2420157.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(12870..12946)
                     /locus_tag="N7B39_000087"
     tRNA            complement(12870..12946)
                     /locus_tag="N7B39_000087"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12910..12912),aa:Arg,seq:acg)
     gene            complement(13049..13125)
                     /locus_tag="N7B39_000088"
     tRNA            complement(13049..13125)
                     /locus_tag="N7B39_000088"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13089..13091),aa:Arg,seq:acg)
     gene            complement(13188..13264)
                     /locus_tag="N7B39_000089"
     tRNA            complement(13188..13264)
                     /locus_tag="N7B39_000089"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13228..13230),aa:Arg,seq:acg)
     gene            complement(13329..13405)
                     /locus_tag="N7B39_000090"
     tRNA            complement(13329..13405)
                     /locus_tag="N7B39_000090"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13369..13371),aa:Arg,seq:acg)
     gene            complement(13409..13501)
                     /locus_tag="N7B39_000091"
     tRNA            complement(13409..13501)
                     /locus_tag="N7B39_000091"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13465..13467),aa:Ser,seq:gct)
     gene            complement(13817..14002)
                     /gene="csrA"
                     /locus_tag="N7B39_000092"
     CDS             complement(13817..14002)
                     /gene="csrA"
                     /locus_tag="N7B39_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HGU2420158.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(14237..16867)
                     /gene="alaS"
                     /locus_tag="N7B39_000093"
     CDS             complement(14237..16867)
                     /gene="alaS"
                     /locus_tag="N7B39_000093"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HGU2420159.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFVVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(16995..17495)
                     /gene="recX"
                     /locus_tag="N7B39_000094"
     CDS             complement(16995..17495)
                     /gene="recX"
                     /locus_tag="N7B39_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HGU2420160.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(17563..18624)
                     /gene="recA"
                     /locus_tag="N7B39_000095"
     CDS             complement(17563..18624)
                     /gene="recA"
                     /locus_tag="N7B39_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HGU2420161.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(18704..19201)
                     /gene="pncC"
                     /locus_tag="N7B39_000096"
     CDS             complement(18704..19201)
                     /gene="pncC"
                     /locus_tag="N7B39_000096"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HGU2420162.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITLRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(19346..20431)
                     /gene="mltB"
                     /locus_tag="N7B39_000097"
     CDS             complement(19346..20431)
                     /gene="mltB"
                     /locus_tag="N7B39_000097"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HGU2420163.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDRMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            20686..21248
                     /gene="srlA"
                     /locus_tag="N7B39_000098"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             20686..21248
                     /gene="srlA"
                     /locus_tag="N7B39_000098"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: protein motif:HMM:NF015565.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            21245..22206
                     /gene="srlE"
                     /locus_tag="N7B39_000099"
                     /gene_synonym="gutE"
                     /pseudo
     CDS             21245..22206
                     /gene="srlE"
                     /locus_tag="N7B39_000099"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
     gene            22217..22588
                     /gene="srlB"
                     /locus_tag="N7B39_000100"
                     /gene_synonym="gutB"
     CDS             22217..22588
                     /gene="srlB"
                     /locus_tag="N7B39_000100"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HGU2420164.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            22592..23371
                     /gene="srlD"
                     /locus_tag="N7B39_000101"
     CDS             22592..23371
                     /gene="srlD"
                     /locus_tag="N7B39_000101"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HGU2420165.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGTAYGFGADATSEQSVLALSRGVDEIFVRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            23477..23836
                     /gene="gutM"
                     /locus_tag="N7B39_000102"
     CDS             23477..23836
                     /gene="gutM"
                     /locus_tag="N7B39_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HGU2420166.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIADTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            23903..24676
                     /gene="srlR"
                     /locus_tag="N7B39_000103"
                     /gene_synonym="gutR"
     CDS             23903..24676
                     /gene="srlR"
                     /locus_tag="N7B39_000103"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HGU2420167.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVGFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDADIDPAFRQALEEKGIDVIITGESNE"
     gene            24669..25634
                     /gene="gutQ"
                     /locus_tag="N7B39_000104"
     CDS             24669..25634
                     /gene="gutQ"
                     /locus_tag="N7B39_000104"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HGU2420168.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTVGGTTL
                     QSQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(25631..27145)
                     /gene="norR"
                     /locus_tag="N7B39_000105"
     CDS             complement(25631..27145)
                     /gene="norR"
                     /locus_tag="N7B39_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HGU2420169.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDAAPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRSGDEVILEAQHFAFPEVTLPPPEVAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            27332..28771
                     /gene="norV"
                     /locus_tag="N7B39_000106"
     CDS             27332..28771
                     /gene="norV"
                     /locus_tag="N7B39_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HGU2420170.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEKMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            28768..29901
                     /gene="norW"
                     /locus_tag="N7B39_000107"
     CDS             28768..29901
                     /gene="norW"
                     /locus_tag="N7B39_000107"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HGU2420171.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLLLFPQTWVTDIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKMVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPV"
     gene            complement(30029..32281)
                     /gene="hypF"
                     /locus_tag="N7B39_000108"
     CDS             complement(30029..32281)
                     /gene="hypF"
                     /locus_tag="N7B39_000108"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HGU2420172.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPYLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALEDLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQVVLSQHLGDLSDDGIQTQWREALRLMQNIYNFTPQYVV
                     HDAHPGYVSCQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(32434..32961)
                     /gene="hydN"
                     /locus_tag="N7B39_000109"
     CDS             complement(32434..32961)
                     /gene="hydN"
                     /locus_tag="N7B39_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HGU2420173.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(33110..34120)
                     /gene="ascG"
                     /locus_tag="N7B39_000110"
     CDS             complement(33110..34120)
                     /gene="ascG"
                     /locus_tag="N7B39_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HGU2420174.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSVERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHKRGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            34380..35837
                     /gene="ascF"
                     /locus_tag="N7B39_000111"
     CDS             34380..35837
                     /gene="ascF"
                     /locus_tag="N7B39_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HGU2420175.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDALIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPALPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTLTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNSELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSAQPTVAKEVSLN"
     gene            35846..37270
                     /gene="ascB"
                     /locus_tag="N7B39_000112"
     CDS             35846..37270
                     /gene="ascB"
                     /locus_tag="N7B39_000112"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HGU2420176.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATGFYHRYKEDIALMAEMGFKVFRTSIAWSRPFPQGD
                     ELTPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMQLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNTNNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVFVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(37394..37864)
                     /gene="hycI"
                     /locus_tag="N7B39_000113"
     CDS             complement(37394..37864)
                     /gene="hycI"
                     /locus_tag="N7B39_000113"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HGU2420177.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(37857..38267)
                     /gene="hycH"
                     /locus_tag="N7B39_000114"
     CDS             complement(37857..38267)
                     /gene="hycH"
                     /locus_tag="N7B39_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HGU2420178.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(38264..39031)
                     /gene="hycG"
                     /locus_tag="N7B39_000115"
     CDS             complement(38264..39031)
                     /gene="hycG"
                     /locus_tag="N7B39_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HGU2420179.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(39031..39573)
                     /gene="hycF"
                     /locus_tag="N7B39_000116"
     CDS             complement(39031..39573)
                     /gene="hycF"
                     /locus_tag="N7B39_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HGU2420180.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(39583..41292)
                     /gene="hycE"
                     /locus_tag="N7B39_000117"
     CDS             complement(39583..41292)
                     /gene="hycE"
                     /locus_tag="N7B39_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HGU2420181.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(41310..42233)
                     /gene="hycD"
                     /locus_tag="N7B39_000118"
     CDS             complement(41310..42233)
                     /gene="hycD"
                     /locus_tag="N7B39_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HGU2420182.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVASSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(42236..44062)
                     /gene="hycC"
                     /locus_tag="N7B39_000119"
     CDS             complement(42236..44062)
                     /gene="hycC"
                     /locus_tag="N7B39_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HGU2420183.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLSVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(44059..44670)
                     /gene="hycB"
                     /locus_tag="N7B39_000120"
     CDS             complement(44059..44670)
                     /gene="hycB"
                     /locus_tag="N7B39_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HGU2420184.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(44795..45256)
                     /gene="hycA"
                     /locus_tag="N7B39_000121"
     CDS             complement(44795..45256)
                     /gene="hycA"
                     /locus_tag="N7B39_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HGU2420185.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            45468..45818
                     /gene="hypA"
                     /locus_tag="N7B39_000122"
     CDS             45468..45818
                     /gene="hypA"
                     /locus_tag="N7B39_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HGU2420186.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            45822..46694
                     /gene="hypB"
                     /locus_tag="N7B39_000123"
     CDS             45822..46694
                     /gene="hypB"
                     /locus_tag="N7B39_000123"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HGU2420187.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAC
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            46685..46957
                     /gene="hypC"
                     /locus_tag="N7B39_000124"
     CDS             46685..46957
                     /gene="hypC"
                     /locus_tag="N7B39_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HGU2420188.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            46957..48078
                     /gene="hypD"
                     /locus_tag="N7B39_000125"
     CDS             46957..48078
                     /gene="hypD"
                     /locus_tag="N7B39_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HGU2420189.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     QRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            48075..49085
                     /gene="hypE"
                     /locus_tag="N7B39_000126"
     CDS             48075..49085
                     /gene="hypE"
                     /locus_tag="N7B39_000126"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HGU2420190.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPTNIHWG
                     AQTLTAGDILLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            49159..51237
                     /gene="flhA"
                     /locus_tag="N7B39_000127"
     CDS             49159..51237
                     /gene="flhA"
                     /locus_tag="N7B39_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HGU2420191.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(51274..51627)
                     /gene="ygbA"
                     /locus_tag="N7B39_000128"
     CDS             complement(51274..51627)
                     /gene="ygbA"
                     /locus_tag="N7B39_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HGU2420192.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKCP
                     VPELPEKYRPKKPRE"
     gene            complement(51704..51838)
                     /locus_tag="N7B39_000129"
     CDS             complement(51704..51838)
                     /locus_tag="N7B39_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2420193.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSVG
                     "
     gene            51914..54475
                     /gene="mutS"
                     /locus_tag="N7B39_000130"
     CDS             51914..54475
                     /gene="mutS"
                     /locus_tag="N7B39_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HGU2420194.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            54581..55237
                     /gene="pphB"
                     /locus_tag="N7B39_000131"
     CDS             54581..55237
                     /gene="pphB"
                     /locus_tag="N7B39_000131"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HGU2420195.1"
                     /translation="MPSTRYQKINTHHYRHIWVVGDIHGEYQLLQSRLHQLSFFPETD
                     LLISVGDNIDRGPESLNVLRLLNQPWFISVKGNHEAMALDAFATGDGNMWLASGGDWF
                     FDLNDSEQKEATDLLLKFHHLPHIIEITNDNIKYVIAHADYPGSEYLFGKEIAESELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIK
                     "
     gene            complement(55278..55514)
                     /locus_tag="N7B39_000132"
     CDS             complement(55278..55514)
                     /locus_tag="N7B39_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit D"
                     /protein_id="HGU2420196.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWTVYQCQHCLYTWRDTEPLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLAEGKR"
     gene            complement(55525..56952)
                     /locus_tag="N7B39_000133"
     CDS             complement(55525..56952)
                     /locus_tag="N7B39_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit C"
                     /protein_id="HGU2420197.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFTDARVAMNTIGSWQNHAISLGLPPNTPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGDEINLFDILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRTRPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTAMLAARK"
     gene            complement(56952..57545)
                     /locus_tag="N7B39_000134"
     CDS             complement(56952..57545)
                     /locus_tag="N7B39_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HGU2420198.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAATISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPYARRWQGLPQARNFSQENE"
     gene            57692..58099
                     /locus_tag="N7B39_000135"
     CDS             57692..58099
                     /locus_tag="N7B39_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HGU2420199.1"
                     /translation="MTLRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDAADKRRRFVWLTAEGEKILAAA
                     IPIGDSVDEEFLGRLSAEEQELFVQLVRKMMNT"
     gene            complement(58219..59210)
                     /gene="rpoS"
                     /locus_tag="N7B39_000136"
                     /pseudo
     CDS             complement(58219..59210)
                     /gene="rpoS"
                     /locus_tag="N7B39_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
     gene            complement(59273..60319)
                     /gene="nlpD"
                     /locus_tag="N7B39_000137"
     CDS             complement(59273..60319)
                     /gene="nlpD"
                     /locus_tag="N7B39_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HGU2420200.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPAHTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGSYSGSTYTVKKGDTLFY
                     IAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITGGNAITQADAAEQGVVI
                     KPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVTAPVTVPTASTTEPTVS
                     STSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQAIIATADGRVVYAGNAL
                     RGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATMGSTGTSSTRLHFEIRY
                     KGKSVNPLRYLPQR"
     gene            complement(60504..61130)
                     /gene="pcm"
                     /locus_tag="N7B39_000138"
     CDS             complement(60504..61130)
                     /gene="pcm"
                     /locus_tag="N7B39_000138"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HGU2420201.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(61124..61885)
                     /gene="surE"
                     /locus_tag="N7B39_000139"
     CDS             complement(61124..61885)
                     /gene="surE"
                     /locus_tag="N7B39_000139"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HGU2420202.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(61866..62915)
                     /gene="truD"
                     /locus_tag="N7B39_000140"
     CDS             complement(61866..62915)
                     /gene="truD"
                     /locus_tag="N7B39_000140"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HGU2420203.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELVELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(62912..63391)
                     /gene="ispF"
                     /locus_tag="N7B39_000141"
     CDS             complement(62912..63391)
                     /gene="ispF"
                     /locus_tag="N7B39_000141"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HGU2420204.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(63391..64101)
                     /gene="ispD"
                     /locus_tag="N7B39_000142"
     CDS             complement(63391..64101)
                     /gene="ispD"
                     /locus_tag="N7B39_000142"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HGU2420205.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVYA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGAAITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(64120..64431)
                     /gene="ftsB"
                     /locus_tag="N7B39_000143"
     CDS             complement(64120..64431)
                     /gene="ftsB"
                     /locus_tag="N7B39_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HGU2420206.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(64625..64948)
                     /gene="ygbE"
                     /locus_tag="N7B39_000144"
     CDS             complement(64625..64948)
                     /gene="ygbE"
                     /locus_tag="N7B39_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HGU2420207.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLMTVGIMFGALF
                     MWLLG"
     gene            complement(64998..65603)
                     /gene="cysC"
                     /locus_tag="N7B39_000145"
     CDS             complement(64998..65603)
                     /gene="cysC"
                     /locus_tag="N7B39_000145"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HGU2420208.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(65603..67030)
                     /gene="cysN"
                     /locus_tag="N7B39_000146"
     CDS             complement(65603..67030)
                     /gene="cysN"
                     /locus_tag="N7B39_000146"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HGU2420209.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRSRVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGEK"
     gene            complement(67032..67940)
                     /gene="cysD"
                     /locus_tag="N7B39_000147"
     CDS             complement(67032..67940)
                     /gene="cysD"
                     /locus_tag="N7B39_000147"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HGU2420210.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHQNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            68192..69229
                     /gene="iap"
                     /locus_tag="N7B39_000148"
     CDS             68192..69229
                     /gene="iap"
                     /locus_tag="N7B39_000148"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HGU2420211.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPQSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     repeat_region   69311..69523
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=69372..69401
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaacaca"
     gene            complement(69619..69912)
                     /gene="cas2e"
                     /locus_tag="N7B39_000149"
     CDS             complement(69619..69912)
                     /gene="cas2e"
                     /locus_tag="N7B39_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HGU2420212.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(69909..70832)
                     /gene="cas1e"
                     /locus_tag="N7B39_000150"
     CDS             complement(69909..70832)
                     /gene="cas1e"
                     /locus_tag="N7B39_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HGU2420213.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPPPARRSVDQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSTKLTGKLIPLIEKVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRGG"
     gene            complement(70829..71479)
                     /gene="cas6e"
                     /locus_tag="N7B39_000151"
     CDS             complement(70829..71479)
                     /gene="cas6e"
                     /locus_tag="N7B39_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311637.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HGU2420214.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREELQGAFRFFVLSQERPAESETFTIECRSFVPELRTGQQLCFNLRANPTICKA
                     GKRHDLLMEAKRQVRGQAEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEV"
     gene            complement(71461..72207)
                     /gene="cas5e"
                     /locus_tag="N7B39_000152"
     CDS             complement(71461..72207)
                     /gene="cas5e"
                     /locus_tag="N7B39_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HGU2420215.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSAPD
                     LLSAIISRRDYYTDAWWMVAVATTPDAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNASDVLRNAYQQYQDSFRELKVSLPKLQDECWWEGEHDGLVASKILRRRDV
                     PLNRQQWLFGERTINQGPWLSKEEPCTSQE"
     gene            complement(72218..73273)
                     /gene="cas7e"
                     /locus_tag="N7B39_000153"
     CDS             complement(72218..73273)
                     /gene="cas7e"
                     /locus_tag="N7B39_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HGU2420216.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEEKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRMAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTLRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTEQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            complement(73285..73821)
                     /gene="casB"
                     /locus_tag="N7B39_000154"
                     /gene_synonym="cse2"
     CDS             complement(73285..73821)
                     /gene="casB"
                     /locus_tag="N7B39_000154"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HGU2420217.1"
                     /translation="MSIVKEEHKATLRKWHEELQEKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIAALSANVKAIDERLPFAAQLAAVMSKGRFTRLS
                     AVKTPDELLRQLRRVVRLLNGSVNLDSLAEGVFRWCQESDDLLNHHRRHQRPTEFIRI
                     RWALEYYQAGDVDNEQNQ"
     gene            complement(73818..75380)
                     /gene="casA"
                     /locus_tag="N7B39_000155"
                     /gene_synonym="cse1"
     CDS             complement(73818..75380)
                     /gene="casA"
                     /locus_tag="N7B39_000155"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HGU2420218.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAAWQFLLGLLQSSFAPKDYRRWDDIWEDGLEAEKLREALLSLEHPFQFGPDSPSFM
                     QDFEVLMGDKVQVASLLPEIPGAQTTKFNKDHFIKRGVTEHVCSHCSALALFSLQLNA
                     PSGGKGYRTGLRGGGPMTTLIELQEYQGNQQAPLWRKLWLNVMPQDEADLPLPKKFDD
                     LVFPWLGPTRTSELAGAVVTDDQVNKLQAYWGMPRRIRIDFNTTTVGNCDICGEQSDA
                     LLSLMTTKNYGANYAMWQHPLTPYRVPLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHHFPLLLNKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRLVRQIE
                     EGQDADELLGKWQKEIWLFARQDFDERVFTNPYEPVDLERVMTARKKYFTTSAEKQSA
                     KAAREKKQEAAE"
     gene            complement(75478..>78017)
                     /gene="cas3"
                     /locus_tag="N7B39_000156"
     CDS             complement(75478..>78017)
                     /gene="cas3"
                     /locus_tag="N7B39_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HGU2420219.1"
                     /translation="RNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQL
                     YLAPELKIPEGSRKNYEKISHSTLGYWLWNYYLSECEELLPSSSLSSRKLTRVIEMWM
                     SITTGHHGRPPDRIDELDNFLPEDKAAARDFLLEIKALFPLIEIPTFWDDDEGVELLK
                     QLSWYISATVVLADWTGSSTRFFPRVAHPMDIKDYWQKTLVQAQNALTVFPPKAETAP
                     FTGINTLFPFIEHPTPLQQKVLDLDISQPGPQLFILEDVTGAGKTEAALILAHRLMAA
                     RKAQGLFFGLPTMATANAMYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWS
                     GDLVGSEEPDEQTFSQGCAAWFANSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLG
                     LSNKILLADEIHACDAYMSCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARG
                     TEGQQEAPFLEKDDYPWLTHVTKSDVNSHRVATRKDVERSVSVGWLHSEQESIARIES
                     AVSQGKCIAWIRNSVDDAIKVHRQLLARGVIPASSLSLFHSRFAFSDRQRIEMETLAR
                     FGKEDGSQRAGKVLICTQVLEQSVDCDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQ
                     LKRDGKDERSPPELLILAPVWDDAPGDEWFGSAMRNSAYVYPDHGRIWLTQRVLREQG
                     AIQMPHAARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFKPGYAAN
                     INDYLPEKLSTRLAEESVSLWLATCIAGVVKPYATGAHAWEMSVVRVRRSWWKKHRDE
                     FSLLEGEAFRQWCIEQRQDPEMANVILVTDDESCGYSAREGLIGKVD"
BASE COUNT        18345 a        21346 c        20051 g        18275 t
ORIGIN      
        1 aaaaaaccgg gctatcgctt ggcgtaaaag acgccagtct ggccattgaa gaaggcgaga
       61 tatttgtcat catgggatta tccggctcgg gtaaatccac aatggtacgc cttctcaatc
      121 gcctgattga acccacccgc gggcaagtac tgattgatgg tgtggatatt gccaaaatat
      181 ccgacgccga actccgtgag gtgcgcagaa aaaagattgc gatggtcttc cagtcctttg
      241 ccttaatgcc gcatatgacc gtgctggaca atactgcgtt tggtatggaa ttggccggaa
      301 ttaatgccga agaacgccgg gaaaaagccc ttgatgcact gcgtcaggtc gggctggaaa
      361 attatgccca cagctacccg aatgaactct ctggcgggat gcgtcaacgt gtgggattag
      421 cccgcgcgtt agcgattaat ccggatattt tattaatgga tgaagccttc tcggcgctcg
      481 atccattaat tcgcaccgag atgcaggatg agctggtaaa attacaggcg aaacatcagc
      541 gcaccattgt ctttatttcc cacgatctcg atgaagccat gcgtattggc gaccgaattg
      601 ccattatgca aaatggtgaa gtggtacagg tcggcacacc ggatgaaatt atcaataatc
      661 cggcgaatga ttatgtccgt accttcttcc gtggcgttga tattagtcag gtattcagtg
      721 cgaaagatat tgcccgccgg acaccgaatg gcttaattcg taaaacccct ggcttcggcc
      781 cacgttcggc actgaaatta ttgcaggatg aagatcgtga atatggctac gttatcgaac
      841 gcggtaataa gtttgtcggc gcagtctcca tcgattcgct taaaaccgcg ttaacgcagc
      901 agcaaggtct tgatgcggcg ctgattgatg cgccgttagc agtcgatgca caaacgcctc
      961 ttagcgagtt gctctctcat gtcggacagg ctccctgtgc ggtgcccgtg gtcgacgagg
     1021 accaacagta tgtcggcatc atttcgaaag gaatgctgct gcgcgcttta gatcgtgagg
     1081 gggtaaataa tggctgatca aaataatccg tgggatacca cgccagcggc ggacagtgcc
     1141 gcgcaatccg cagacgcctg gggtacaccg gcgactgcac cgactgacgg cggtggtgct
     1201 gactggctga ccagtacgcc tgcgccaaac gtcgagcatt ttaatattct cgatccgttc
     1261 cataaaacgc tgatcccgct cgacagttgg gtcactgaag ggatcgactg ggtcgttacc
     1321 catttccgtc ccgtcttcca gggcgtgcgc gttccggttg attatatcct caacggtttc
     1381 cagcaattgc tgctgggtat gcccgcaccg gtggcgatta tcgttttcgc tctcatcgcc
     1441 tggcagattt ccggggtcgg aatgggcgtg gcgacgctgg tttcgctgat tgccatcggc
     1501 gcaatcggtg cctggtcgca ggcaatggtt actctggcgc tggtgttaac cgccctgctg
     1561 ttctgtatcg tcatcggttt gccgttgggg atctggctgg cgagaagtcc gcgagcggcg
     1621 aaaattattc gtccactgct tgatgccatg cagaccacgc cagcgtttgt ttatctggtg
     1681 ccaatcgtca tgctatttgg tatcggtaac gtgccgggcg tggtggtgac gatcatcttt
     1741 gctctgccgc cgattatccg tctgaccatt ctggggatta accaggttcc ggcggatctg
     1801 attgaagcct cgcgctcatt cggtgccagc ccgcgccaga tgctgttcaa agttcagtta
     1861 ccgctggcga tgccgaccat tatggcgggc gttaaccaga cgctgatgct ggccctttct
     1921 atggtggtca tcgcctcgat gattgccgtc ggcgggttgg gtcagatggt acttcgtggt
     1981 atcggtcgtc tggatatggg gcttgccacc gttggcggcg tcgggattgt gatcctcgcc
     2041 attatcctcg atcgtctgac gcaggccgtt gggcgcgact cacgcagtcg cggcaaccgt
     2101 cgctggtaca ccactggccc tgtcggtctg ctgacccgcc cattcattaa gtaactctgc
     2161 acttgcccgg tgacgccggg catatcaccc tgccaaaaaa aggaataaca atgcgacata
     2221 gcgtactttt tgcgacagcg tttgccacgc ttatctctac acaaactttt gctgccgacc
     2281 tgccgggcaa aggcattact gttaatccgg ttcagagcac catcaccgaa gaaactttcc
     2341 agacgctgct ggtcagccgc gcgctggaga aattaggtta taccgtcaat aaacccagcg
     2401 aagtggatta caacgttggc tacacctcgc ttgcttccgg cgatgcaacc ttcaccgccg
     2461 tgaactggac gccactgcat gacaacatgt acgaagctgc cggtggcgat aagaaatttt
     2521 atcgtgaagg ggtatttgtt aacggcgcgg cacagggtta cctgatcgat aagaaaaccg
     2581 ccgaccagta caaaatcacc aacatcgcac aactgaaaga tccgaagatc gccaaactgt
     2641 tcgataccaa cggcgacgga aaagcggatt taaccggttg taaccctggc tggggctgcg
     2701 aaggtgcgat caaccaccag cttgccgcgt atgaactgac caataccgtg acgcataatc
     2761 aggggaacta cgcggcgatg atggccgaca ccatcagtcg ttacaaagag ggcaaaccgg
     2821 tgttttacta cacctggacg ccgtactggg tgagtaatga gctgaagcca ggcaaagatg
     2881 tggtctggtt gcaggtgccg ttctctgcac tgccgggcga taaaaatgcc gataccaaac
     2941 tgccgaatgg cgcgaattac ggcttcccgg tcagcaccat gcatatcgtt gccaataaag
     3001 cctgggccga gaaaaacccg gcagcagcga aactgtttgc cattatgcag ttgccagtgg
     3061 cagatattaa cgcccagaac gccattatgc atgacggcaa agcctcagaa ggcgatattc
     3121 agggccacgt tgatggctgg atcaaagccc accagcaaca gttcgatggc tgggtgaatg
     3181 aggcgttggc agcgcagaag taatttttat tcgggcggat aaggcgttta cgccgcatcc
     3241 gccgttctgt acacaatgcc tgatgcgacg ctggcgcgtc ttatcatgcc tacaaaccac
     3301 accgcacatt tcaacaatct atctttcatc ccatattcat caacatccgc tattattgat
     3361 ttccagctta atcatcacct gatgaacaaa aataatgacc aaacctaatc atgagcttag
     3421 cccggcgcta atcgtgctga tgtctatcgc caccggtctg gcggtcgcca gcaactatta
     3481 cgcccagcca ttgctcgaca ccatcgcgcg taacttttcc ctttccgcca gttcggcagg
     3541 ctttattgtt accgccgcgc agttgggcta tgccgccggt ctgctgtttc ttgttcccct
     3601 cggtgatatg tttgaacgcc gccgcctgat tgtctcgatg accttactgg cagcgggcgg
     3661 tatgttaatc actgccagca gtcagtcact ggcgatgatg atcctcggta cggcattaac
     3721 cggtttattc tcagtcgtgg cacaaattct ggttccgctg gcagcgacgc tggcttcacc
     3781 ggataaacgc ggcaaagtgg ttggcaccat tatgagcggt ctgctgttgg ggatattgct
     3841 ggcacgaacc gttgccgggt tactggcaaa cctcggcggc tggcgcaccg tcttttgggt
     3901 tgcctcggtg ttaatggctc tgatggcgct ggcattatgg cgtggtctgc cacaaatgaa
     3961 atcagaaacc cacctcaact acccacagtt gttgggttcc gttttcagca tgtttatcag
     4021 caataagatc ctgcgcaccc gcgcgttgct gggctgcctg acctttgcca acttcagcat
     4081 tctctggacc tcaatggcct ttttgcttgc cgctccacct tttaactaca gcgatggtgt
     4141 aattggtctg tttgggcttg cgggagctgc cggagcgttg ggcgctcgtc cggcgggcgg
     4201 ttttgccgac aagggcaaat cgcaccacac cacaactttc ggtctgcttc tgctgttact
     4261 ttcatggctg gcaatctggt ttggtcacac ttccgtactg gcgttgatta tcggcattct
     4321 ggtgctggac ctcaccgtac agggcgtgca tatcactaac cagacggtaa tttatcgaat
     4381 acatcctgat gcgcgtaatc gcctgaccgc aggttacatg accagctact ttattggcgg
     4441 tgccgccggt tcgctaattt cagcctcagc ctggcaacat ggcggttggg ctggcgtttg
     4501 tctggctggc gcgacgattg ccctggttaa cttactggtc tggtggcgag gttttcatcg
     4561 tcaggaagcc gcaaattaag caatcagcaa ccgttttcgt ggctttacac ttataagggt
     4621 gttaagaagc ccatcagtct gataaggtta agatattcat tcagtctatt tataatatta
     4681 acaatcgtta agcgtacact ctatggaaag ccctactcca cagcctgctc ctggttcggc
     4741 gaccttcatg gaaggatgca aagacagttt accgattgtt attagttata tcccggtggc
     4801 ctttgcgttc ggtctgaatg cgacccgtct gggattctct cctctcgaaa gcgttttttt
     4861 ctcctgcatc atttatgcag gcgcgagcca gttcgtcatt accgcgatgc tggcagcagg
     4921 gagtagtttg tgggttgctg cactgaccgt catggcaatg gatgttcgcc atgttttgta
     4981 tggcccgtca ctgcgtagcc gtattattca gcgtctgcaa aaatcgaaaa ccgccctatg
     5041 ggcgtttggc ctgacggatg aggtttttgc cgccgccacc gcaaaactgg tacgcaataa
     5101 tcgccgctgg agcgagaact ggatgatcgg cattgccttc agttcatggt catcgtgggt
     5161 atttggtacg gtaatagggg cattctccgg cagcggcttg ctgcaaggtt atcccgccgt
     5221 tgaagctgca ttaggtttta tgcttccggc actctttatg agtttcctgc tcgcctcttt
     5281 tcagcgcaaa caatctcttt gcgttaccgc agcgttagtt ggtgcccttg caggcgtaac
     5341 gctattttct attcccgtcg ccattctggc aggcattgtc tgtggctgcc tcactgcgtt
     5401 aatccaggca ttctggcaag gagcgcccga tgagctatga ggttctgctg cttgggttac
     5461 tggttggcgc ggcgaattat tgcttccgct atttgccgct gcgcctgcgt gtgggtaatg
     5521 cccgcccaac caaacgtggc gcggtaggta ttttgctcga caccattggc atcgcctcga
     5581 tatgcgctct gctggttgtc tctaccgcac cagaagtgat gcacgataca cgccgtttcg
     5641 tgcccacgct ggtcggcttc gcggtactgg gtgccagttt ctataaaaca cgcagcatta
     5701 tcatcccaac actgcttagt gcgctggcct atgggctcgc ctggaaagtg atggcgatta
     5761 tataacccac aagaatcatt tttctaaaac aatacattta ctttatttgt cactgtcgtt
     5821 actatatcgg ctgaaattaa tgaggtcata cccaaatgga tagttcgttt acgcccattg
     5881 aacaaatgct aaaatttcgc gccagccgcc acgaagattt tccttatcag gagatccttc
     5941 tgactcgtct ttgcatgcat atgcaaagca agctgctgga gaaccgcaat aaaatgctga
     6001 aggctcaggg gattaacgag acgttgttta tggcgttgat tacgctggag tctcaggaaa
     6061 accacagtat tcagccttct gaattaagtt gtgctcttgg atcatcccgt accaacgcga
     6121 cgcgtattgc cgatgaactg gaaaaacgcg gttggatcga acgtcgtgaa agcgataacg
     6181 atcgccgctg cctgcatctg caattaacgg aaaaaggtca cgagtttttg cgcgaggttt
     6241 taccaccgca gcataactgc ctgcatcaac tctggtccgc gctcagcaca acagaaaaag
     6301 accatctcga gcaaatcacc cgcaaattgc tctcccgtct cgaccagatg gaacaagacg
     6361 gtgtggttct cgaagcgatg agctaacgcg tcatctcgct caaaaatcca gatttataaa
     6421 agaaaaaatg accggccagc atcgcaacat gctggccttt ttggcaagca ggtcggctca
     6481 gccgatgagt taagaagatc gtggagaaca atatgagcgc aaatgcggag actcaaaccc
     6541 cgcagcaacc ggtaaagaag agcggcaaac gtaagcgtct gctcctcctt ctcaccttgc
     6601 tctttataat tattgccgta gcgataggga tttattggtt tttggtactg cgtcacttcg
     6661 aagaaaccga tgacgcatac gtggcaggga atcaaattca aattatgtct caggtgtctg
     6721 gcagcgtgac gaaagtctgg gccgataaca ccgattttgt aaaagaaggc gacgtgctgg
     6781 tcactctcga cccgacagat gctcgccagg cgtttgaaaa agccaaaact gcactggctt
     6841 ccagcgttcg ccaaacccac cagcagatga ttaacagcaa gcagttgcag gcgaatattg
     6901 aggtgcaaaa aatcgccctc gcgaaagcac aaagcgacta caaccgccgt gtgccgctgg
     6961 gcaatgccaa cctgattggt cgcgaagagc tgcaacacgc ccgcgacgcc gttaccagtg
     7021 cccaggcgca actggacgtc gcgattcaac aatacaatgc caatcaggcg atgattctgg
     7081 ggactaaact ggaagatcag cctgccgtgc aacaggctgc caccgaagta cgtaacgcct
     7141 ggctggcgct ggagcgtact cgtattgtca gtccgatgac cggttatgtc tcccgccgcg
     7201 cggtacagcc tggggcgcaa attagcccaa cgacgccgct gatggcggtc gttccagcca
     7261 ccaatatgtg ggtggatgcc aactttaaag agacgcagat tgccaatatg cgtatcggtc
     7321 agccggtcac tatcaccacc gatatttacg gcgatgatgt gaaatacacc ggtaaagtgg
     7381 ttggtctgga tatgggcaca ggtagcgcgt tctcactgct tccggcgcaa aatgcgaccg
     7441 gtaactggat caaagtcgtt cagcgtctgc ctgtgcgtat cgaactggac cagaaacagc
     7501 tggagcaata cccgctgcgt atcggtttat ccacgctggt gagcgtcaat accactaacc
     7561 gtgacggtca ggtactggca aataaagtac gttccactcc ggtagcggta agcaccgcgc
     7621 gtgaaatcag cctggcacct gtcaataaac tgatcgacga tatcgtaaaa gctaacgctg
     7681 gctaatccag aggtgcgtgt gatgcaacag caaaaaccgc tggaaggcgc gcaactggtc
     7741 attatgacga ttgcgctgtc actagcgaca ttcatgcagg tgctggactc caccattgct
     7801 aacgtggcga tccccactat cgccgggaat cttggctcat cgctcagcca gggaacgtgg
     7861 gtaatcactt ctttcggggt ggcgaatgcc atctcgatcc cgcttaccgg ctggctggca
     7921 aagcgcgtcg gggaagtgaa actgttcctc tggtcaacca tcgcctttgc tattgcgtcg
     7981 tgggcgtgtg gtgtctccag cagcctgaat atgctgatct tcttccgcgt gattcagggg
     8041 attgtcgccg ggccgttgat cccgctttcg caaagtctat tgctgaataa ttacccgcca
     8101 gccaaacgct caatcgcgct ggcgttgtgg tcgatgacgg tgattgtcgc gccaatttgc
     8161 ggcccaatcc tcggcggata tatcagcgat aattaccact ggggctggat attcttcatc
     8221 aacgtgccga ttggcgtggc ggtggtgttg atgacactgc aaactctgcg cggacgtgaa
     8281 acccgcaccg aacggcggcg gattgatgcc gtggggctgg cactgctggt tattggtatc
     8341 ggcagcctgc agattatgct cgaccgcggt aaagagctgg actggttttc atcacaggaa
     8401 attatcatcc ttaccgtggt ggcggtggtg gctatctgct tcctgatcgt ctgggagctg
     8461 accgacgata acccgatagt cgatctgtcg ttgtttaagt cgcgcaactt caccatcggc
     8521 tgtttgtgta tcagcctcgc gtatatgctc tacttcggcg cgattgttct gctgccgcag
     8581 ttgttgcagg aggtctacgg ttacacggcg acctgggcag gtttggcctc tgcgccggta
     8641 gggattattc cagtgatcct gtcgccgatt atcggccgct tcgcgcataa actggatatg
     8701 cgccgactgg taaccttcag ctttattatg tatgccgtct gcttctactg gcgtgcctat
     8761 acctttgaac caggtatgga ttttggcgcg tcggcctggc cgcagtttat ccaggggttt
     8821 gcggtggcct gcttctttat gccgctgacc accattacgc tgtctggttt gccaccggaa
     8881 cgactggcgg cggcatcgag cctctccaac tttacgcgta cgctggcggg gtctatcggc
     8941 acgtcgataa ccacaaccat gtggaccaac cgcgagtcga tgcaccatgc gcagttgact
     9001 gagtcggtaa acccgttcaa cccgaatgcc caggcgatgt acagtcaact ggaagggctt
     9061 gggatgacac aacagcaggc atcaggctgg attgcccagc agatcaccaa tcaggggctg
     9121 attatttccg ccaatgagat cttctggatg tcggccggga tattcctcgt cttgctgggg
     9181 ctggtgtggt ttgctaaacc gccctttggc gcaggtggcg gcggaggcgg tgcgcactaa
     9241 gtacaactaa gccagttcat ttgaactggc ttttttcaat taattgtgaa gatagtttac
     9301 tgactaaatg tgcagttcct gcaacttctc tttcggcagc gccagttctt cgttgctgtt
     9361 gatgcgcacg tcacgttcca gaatgctacg cgcaatatcc tgcgcttcct gcaacgagtg
     9421 catctggtaa gtgccacact ggtagacgtt cagctccgga atctgattct gatcctgcac
     9481 tttcagcacg tcttccattg ccgctttcca gacatcagca acacgctgct catctggcgt
     9541 accaatcaga ctcatataaa aaccggtgcg gcagcccatt ggcgagatat cgataatctc
     9601 tacaccatta ccgttaagat ggttacgcat aaaaccagca aacaggtgct ccagggtatg
     9661 gatccctctt tctggcatca cttctttgtt cggcacgcag aagcgcagat cgaacacggt
     9721 gattgcgtcg ccatgcgggg tgttcattgt tttcgccacc cgaactgcag gcgcttccat
     9781 ccgggtatga tcgactgtga agctatctaa caacggcatt tagccacctc cggtaatttt
     9841 tttaaaaatt ttctgaactc tttcttccca ggcgagtctg agtatatgaa agacgcgcat
     9901 ttgttatcat catccctgaa ttcagagatg aaattttggc cactcatgag tggccttttt
     9961 cttttctgtc aggcgtgttt ttccagccac accgcaaacg gttcggtatc tgcggcttcc
    10021 atttcctgct gacggcgttc agatgcctcg cgctcggcta caaaatcctc ttcgcgcaga
    10081 atttccagcg gctcttcacg cagcagatta cggtacgctt cagcaaacgc tttgcctgtt
    10141 ccgccaatac cagtatcaat catagacctt aagatacggg cagagaaagt cagatcggga
    10201 ttatcgaagc aggcaaccag ttcatcacac actttctgat acgcttcgcc gccgttaatg
    10261 ctatccagcg tttgcgcgac gcgtttcaga tcgcggaaca gatctttacc cacctgcggt
    10321 aacgggaact gtgcggtttc gcagccgata cccagcgtca gacccggttt gcgaccttcg
    10381 aggatcaccc ggttccagtt aacgcgtgta caggcaagtt cgctactgct catttccggt
    10441 gcatcagcca gcgcacacca gaccataaac aggtcgagga atcgcacctg ctgttcatct
    10501 acaccaatcg gcgagaacgg gttgatgtcc agcgaacgca cttcaatata ttcaatgccg
    10561 ccacgtaaca gcgcatcaga aggcgactcg ccgctgcggg taacgcgttt tggacgaatc
    10621 ggcgcgtaca gttcgttttc aatctgcagc acgttgctgt tgatttgcag cctcttaccg
    10681 tctttatcaa taccaatctt cgcgtactct tccgatggcg ttttgattgc ctgtttcagg
    10741 cccgctacgt actcgtaaag atcgttgaag gtaataccaa gattgctttg cgatttattg
    10801 gtatagccga gatcgctcaa acgaagagag gtcgcatacg gcaggtaata cataccgcac
    10861 tcggttttct caaacggcag cgacgttggt ttaccttgca ggaatgaaga gcatattgcc
    10921 ggagacgcgc caaacagata aggaatgacc caaccgaagc gatagtagtt gcgaataacg
    10981 cggaaatagc ccgcagagat tttctcttta gcatcagcgc ccgaaatatc gccacacttc
    11041 gcttgccaga atgccatagg caaagagaag ttgtaatgca cgccggagat ggtttgcatc
    11101 agcgcgccgt agcgattttt cagcccttca cggtacagcg ttttaaagcg tccggtgtta
    11161 gaagtgccgt actgcgccag ttcgatgtcc tgaccttctg cgatgtagca tggcatactt
    11221 aacggccaca tccgctcatc gcccatattg cgcgccgtat aacgatgcag atcgcgcata
    11281 aaggtcagca tatgttcaat atcaccatcc actggtgtaa tgaattccag caatgcttcc
    11341 gcaaaatcgg tagtaatcca tttatgcgtc agtgcggaac ctaatgcttc aggatgacct
    11401 gttgttgcca gtgtgccatc agcattaaca cgcaaagttt cgcgctccag cccacgctgt
    11461 atccccttta acgcctgagg atgtttttcc agccaggcca gcgcctgtga tacgtccggg
    11521 atcaaattga cctcccgcct gtcaaaatcg ttttaattag cataactgta atggtgacca
    11581 tatgtgcagg cctacaatta gtgccaccac atcatgccct gaacggtcgc tgctgcaact
    11641 gcaacatagc gtaacgcttt accaaggcac aaaaaaaaga ttaccggccc ccaggagatg
    11701 cgcatccatc ccgctaacag acacagtaaa tcgccaacca cgggcatcca gcttaataat
    11761 agcgtgactg caccatagcg tttcagccag ccggtagctt tctcttgcca gcgcgatgtt
    11821 ttacgcaatg gacagaaacg cccaaggata acgttagtta accctccaag gctattaccc
    11881 attgttgctg ttaaaactaa aacccaggga tgactgatcc cagaaagcaa cattgccacc
    11941 agcacgactt cggagttgcc gggtaatagt gtagcgctga gaaaactact ggcaaacaac
    12001 gagaaaagcg ataacgcttc actcacagca agcgaacatc cacagcgtcc atgcctgctg
    12061 cacgggccgc ctgaataccg aaatcggcat cttcaaagac cacacactgc gtcggttgca
    12121 cgcccatacg ctgcgcgcac aacaaaaatg tgtctggcgc gggtttatgg tgtttgacgt
    12181 gatcggcagc gacgacggcg tcaaaataac ggcgtaatcc caggtgagcc agcaatgcct
    12241 cagcgatggc gctttcactc cccgttccta cagccattgg gcgacgacca tgccaacttt
    12301 tcaccacttc aacaagagga agcggctcga cactatccag cagcatactt cttactgctt
    12361 ctgttttttc acgcgctaac gcatgcgggt cgagatcggc ctgattcagc tcaataattg
    12421 cctgagcaat acgccaggtg ggcgatccat taagcgcaat catcgcctga acatcgtact
    12481 gaagaccgta gtgccctaat acttcgcgcc acgctttacg gtgcgtaggc tccgtatcca
    12541 ggattgtgcc atccatatca aaaattaaac ctgcataacg ctcgtacatg gtcttctcgc
    12601 aagtcgaaaa atatgacgct actttagcgt aattgcttga ttttgtcgct gatggcgaag
    12661 gagaagaaaa ttcaggaaat ggataaagta gttatttaga aatgagatat ttttgaagga
    12721 aattttttgg agaagatggt gcatccggga ggatgactca ctggcgttcg cccttcgggc
    12781 cgttgctaaa gcaacgttat ccttcctggt gcctgcggtt aattccgcta cctcttcgat
    12841 atcttcattg ctgaagattt cggagaatat ggtgcatccg ggaggattcg aacctccgac
    12901 cgctcggttc gtagccgagt actctatcca gctgagctac ggatgcatcg ggaaaattta
    12961 ttttactgct gatattgata tcgctactaa agccatatca aataagaaaa tccatcttaa
    13021 ccatcatcac tgatgattac ggagaatatg gtgcatccgg gaggattcga acctccgacc
    13081 gctcggttcg tagccgagta ctctatccag ctgagctacg gatgcatcgg gaaatttatt
    13141 ttactgctga tattgatacc gctactaaag ctgtatcaag taagagatgg tgcatccggg
    13201 aggattcgaa cctccgaccg ctcggttcgt agccgagtac tctatccagc tgagctacgg
    13261 atgcatcggg aaacttactt tactgcagat tttttgatac cgctactaaa gccgtatcaa
    13321 gtaagagatg gtgcatccgg gaggattcga acctccgacc gctcggttcg tagccgagta
    13381 ctctatccag ctgagctacg gatgcaaatg gcggtgaggc ggggattcga accccggatg
    13441 cagcttttga ccgcatactc ccttagcagg ggagcgcctt cagcctctcg gccacctcac
    13501 cacacgcctc ttacgagtgc ttcgaagaac ttgtttatcg ctcatcgtcg ctgcgtggcg
    13561 cacatattac tttctgagac ttataagtca aacaattttt cccacacttt tatcgtttgc
    13621 acacttcacg ctcaattagt ctgtaaaaac gtcaaaaaga gtgttttatc tacagaaaaa
    13681 cggaggtctg gcagatagca gtaatgcaaa aaaatggaga cttaagttga atgaacggga
    13741 gtaaagcgaa aagactatag agtgaaggag aaattttgag ggtgcgtctc accgataaag
    13801 atgagacgcg gaaagattag taactggact gctgggattt ttcagcctgg atacgctggt
    13861 agatctcttc acggtgaaca gaaacttcct tcggggcatt tacgccaata cgtacctggt
    13921 tgccctttac ccctaaaact gtcacggtga cctcatcccc aatcatgagg gtctcaccaa
    13981 ctcgacgagt cagaatcagc attctttgct ccttgaaaga ttaaaagagt cgggtctctc
    14041 tgtatcccgg cattatccat catataacgc caaaaagtaa gcaatgacaa acacattaca
    14101 tgtaagcagt catggcatta cattctgtta aacctaagtt tagccgatat acacaacttc
    14161 aacctgactt tatcgttgtc gatagcgtta atgcgaatgc cgtgaagcga gtccacggca
    14221 ttgcctgacg cttatattat tgcaatttcg cgctgaccca gcctttcaca ctggctaacg
    14281 ctgcaggtaa ggccgcagca tccgtaccac cggcttgcgc catgtcagga cgtccaccac
    14341 ccttgccgcc cacctgctga gcgaccatac caatcagttc ccctgctttc acacgatctg
    14401 tgacgtcctt agatacgcct gcaatcagag aaaccttacc ttcggctacc gttgccagca
    14461 cgataattgt cgaccccagc tgatttttta aatcgtcaac catggtacgc aacattttcg
    14521 gctcaacacc gctaagctcg ctaaccaaca gcttaacacc attaacatca attgccttac
    14581 tggaaagatt tgcgctctcc tgtgcggcag cttgttcttt aagctgttgt agttcttttt
    14641 ctaactgacg cgtacgttcc agtactgagc gcactttatc cgccagatta ttgctatcgc
    14701 ctttcagcag atgcgcgact tcgcttaagc gatcgctgtc tgcatgaacg gtggtgatag
    14761 cgccttctcc ggttaccgct tcgatacgac gaacgcctgc agcagtaccc gattcagaga
    14821 tgatgcggaa cagaccaata tcaccagtgc ggctggcgtg agtaccgcca cacagctcgg
    14881 tggagaaatc gcccatgctc agcacgcgta cacgctcatc atacttctcg ccgaacagcg
    14941 ccatcgcacc tttcgctttc gccgcttcga gatccatgat gttggtttcg atcggcaaat
    15001 tgcgacgaat ctgtgcgttc accaggtctt cgaccgcacg aatctcttcc ggtttcatcg
    15061 cttcgttgtg tgagaagtcg aagcgcagca ccttgtcgtt aaccagtgaa cctttctgcg
    15121 atacatgagt ccccagaacc tggcgcagcg cagcgtgcat caggtgcgtt gcggagtgat
    15181 tcaaacgaat acgggcgcga cgagcctcat caacatcagc ctgcacagcg tcgcccactt
    15241 tcagagaacc cgcagcaagt ttaccgatgt gaccaatcgc ctggccgtat ttctgcgtat
    15301 cttccaccac gaaggagaag ttagcgcctt tcagttcgcc tttatcacca acctgaccgc
    15361 cggattccgc atagaatggc gtttgatcca gcacgaccac agcttcctgg cctgcattga
    15421 tggcatcaac cgctttacca tcaacaaaca gcgcggtcac tttgccgttc agttccagat
    15481 ggtcatagcc tttaaattca gatgcactgt caacacggat cattgcgttg taatcggcac
    15541 caaagccgct ggcttcgcgc gcccgacgac gctgctcttc cattgctgct tcaaaaccag
    15601 cttcgtcaac tttgatgttg cgctcacgac aaacatcagc cgtcaggtca accgggaagc
    15661 cataggtgtc gtacagacgg aaagcagttt caccatccag cgtatcacca gaaagttttg
    15721 ccagctcttc atccagcaac gccagaccgc gctccagagt acgagcaaac tgctcttctt
    15781 cagtcttcag cacctgctca acctgcgcct gctggcgttt caggtcttca cccgcagagc
    15841 ccataacgtc gatcagcgga ccaaccagtt tgtagaagaa ggtttctttc gcgccgagca
    15901 tattaccgtg acgcactgcg cgacgaatga tacgacgcag tacataacca cggttttcat
    15961 tggacggcat tacgccatcc gcgatcagga acgcacaaga acgaatgtgg tcagcgatta
    16021 cgcgcagcga tttattgctc agatcggttg cgccagtgac tttcgctacc gcctggatca
    16081 acgtgcggaa caggtcgatg tcatagttag agttaacgtg ttgcagcacc gcagcaatac
    16141 gctccagacc cataccggta tctacagacg gcttcggcag cggttccatc gtgccatcgg
    16201 cctggcggtt gaactgcatg aagacgatgt tccagatctc aatgtagcgg tcgccgtctt
    16261 cttccggact tcccggaggg ccaccccaaa tgtggtcgcc gtgatcgtag aagatttcgg
    16321 tgcacgggcc gcacggacca gtgtcaccca tctgccagaa gttgtcagat gcgtatggcg
    16381 cacctttgtt atcgccgatg cgaataatac gttcgcgcgg gatccctact tctttttccc
    16441 agatttcgta ggcttcgtcg tcgctttcat agacggtaac ccacagacgc tctttcggca
    16501 gggcaaacca tttttcgctg gtcagcagtt cccatgcaaa ctgaatggca tcgtgtttga
    16561 aatagtcgcc gaagctgaag ttgcccagca tttcgaagaa ggtatggtga cgcgcggtgt
    16621 aaccgacgtt ttccaggtcg ttgtgtttac cacccgcacg cacgcagcgt tgggaagtgg
    16681 tagcgcggga ataattacgc ttgtcgagcc caaggaacac atccttgaac tggttcatcc
    16741 cggcgttggt aaacaacaaa gttgggtcgt tatggggtac cagggagctg ctggcaacta
    16801 cctgatgtcc cttactatgg aaaaagtcga gaaacgcctg acggatctca gcggtgctct
    16861 tgctcataat tatcctgaaa tcaagctaac gaaatatcgc caccagctcc agcgtgtctt
    16921 aaccgccggg ctggtaactg aaaagtggga ataagataag ttttcttgac tgggaagtaa
    16981 aataccgtat gcgttcagtc ggcaaaattt cgccaaatat cctggatatc ttccatcaga
    17041 tagccacgat agagcagaaa acgctggatc ttaacttttt ctgaaaagac agttggcaaa
    17101 ggttcaccat attttcgcgt cgcctgatcg cgcgccagtg cgcaccagtc gatgtcacat
    17161 tcacgcatcg ctttttctgt cgcttcgcgg gaaatacctt tctgattcag ttcctggcga
    17221 atacgcgcag gtccataacc tttgcggcta cggctggcga taaagcgcgc aacaaatcgg
    17281 ctgtcatcga gatagccatg ttcatggcac caggcaataa cgcgctcgta atcttctgcc
    17341 gtagcatcaa tctcttctgg gccatttttg cccataatcg gtgccgcgag tttacgtcgc
    17401 agttcttgct cactgtgatc gcgcaccgcc agaatgcgta ccgcacgatc caacaggcga
    17461 gcatatgccg ggcgacggga cgttgattct gtcatggcat atccttacaa cttaaaaagc
    17521 aaaagggccg cagatgcgac ccttgtgtat caaacaagac gattaaaaat cttcgttagt
    17581 ttctgctacg ccttcgctat catctacaga gaaatccggc gttgagttcg ggttgctcag
    17641 cagcaactca cgtactttct tctcgatctc tttcgcggtt tccgggttat ctttcagcca
    17701 ggcagttgca ttcgctttac cctgaccgat cttctcacct ttgtagctgt accacgcgcc
    17761 tgctttctcg atcagcttct cttttacgcc caggtcaacc agttcgccgt agaagttgat
    17821 accttcgccg tagaggatct ggaattcagc ctgtttaaac ggcgcagcga ttttgttctt
    17881 caccactttc acgcgggttt cgctacccac cacgttttcg ccctctttca ccgcgccgat
    17941 acgacggatg tcgagacgaa cagaggcgta gaatttcagc gcgttaccac cggtagtggt
    18001 ttccgggtta ccgaacatca caccaatttt catacggatc tggttgatga agatcagcag
    18061 cgtgttggac tgcttcaggt tacccgccag cttacgcatc gcctggctca tcatacgtgc
    18121 cgcaaggccc atgtgagagt cgccgatttc gccttcgatt tccgctttcg gcgtcagtgc
    18181 cgccacggag tcaacgacga taacgtctac tgcgccagaa cgcgccaggg cgtcacagat
    18241 ttccagtgcc tgctcgccgg tgtccggctg ggagcacagc aggttgtcga tatcgacgcc
    18301 cagtttacgt gcgtagattg ggtccagcgc gtgttcagca tcgataaacg cacaggtttt
    18361 accttcacgc tgcgctgcgg caatcacctg caacgtcagc gtggttttac cggaagattc
    18421 cggtccgtag atttcgacga tacggcccat cggcagacca cctgccccaa gcgcgatatc
    18481 cagtgaaagc gaaccggtag agatggtttc cacatccatg gaacggtctt cacccaggcg
    18541 catgatggag cctttaccaa attgtttctc aatctggccc agtgctgccg ccaacgcttt
    18601 ctgtttgttt tcgtcgatag ccatttttac tcctgtcatg ccgggtaata ccggatagtc
    18661 aatatgttct gttgaagcaa ttatactgta tgctcataca gtatcaagtg ttttgtagaa
    18721 attgttgcca caaggtctgc aatgcatacg cagtagcctg acgacgcacc gcatcacggt
    18781 cgccgctgaa gcattcccgc agggtaatgc cttcaccgcg ggcagtggca aaagcaaacc
    18841 agacggtgcc gacaggcttc tcttcactgc cgccatccgg cccggcgata ccactaatag
    18901 acacggcata atcagcacga gccgctttca gtgcacctat cgccatttcc accacgacgg
    18961 gttcactcac cgcgccatgc tgcgccagcg tctcttcgcg tacgccgatc atttgcgctt
    19021 tggcttcgtt actgtaggtg acaaatccgc gttcaaacca ggcggaactg ccggcaatat
    19081 cggtaatcac tttcgctacc caaccgccgg tacaagactc agcagttgtt acggttgcgc
    19141 cacgggcttt cagcgcctgc ccaacctgtt cgcttaactg catcagttca ctgtcagtca
    19201 tcacaacctc tgtcagtcaa aaatttatgc cggacaagat agcactttcg tcaggaagat
    19261 ggtgttggcg ttgcaatttt acgaggagga ttcagaaaaa agctgattag ccagagggaa
    19321 gctcacgccc ccctcttgca aatagttact gcacgcgcgc cagcgccacg gcttgtccta
    19381 actgccagac cgccattgcg taatgggtac tgtggttata acgggtaatg gtgtagaagt
    19441 tcggcagacc gtaccagtac tggtaacctg ttccaacatc cagacgcagc aggctggctt
    19501 gttgatggtt gcccagcggc tgctgtggcg ttaaacctgc cgcggcaagc tgcgaaatgc
    19561 tgtacctggt tttgaagcca tttggcaagc ctggagcctg accgtttgcc attaccgcga
    19621 cctgatcgcc tttcacccag ccgtgcgctt tgaaataatt cgccacgcta ccgatcgcat
    19681 caaccggatc ccacaggttg atatgcccgt cgccgctgaa atctaccgca tattgtttgt
    19741 aagacgacgg cataaactgt ccgtagccca tcgccccggc aaaggaacct ttcagattga
    19801 gcggatcgtc ctgctcgtcg cgcgccatca gcaggaaggt ttccagctcg ccagagaaat
    19861 actccgcgcg gcgtgggtag ttaaatgaca gcgttgccag cgcatcgagg atgcgagttt
    19921 tccccatcac gcgcccccag cgggtttcaa cgccgataat cccgacgata atttccggcg
    19981 gtacgccata cacctgccac gcgcgattca acgcatcttc atactgattc cagaaaacca
    20041 caccgttctg cacattgtcc ggcgtaataa attttttgcg ataacgcagc catgcgccgt
    20101 tcggacctga tggcggtttc accgatgtgg ttggtgcctg gttatccatc agccgcagta
    20161 ccgaatccag acgtttcgcc tgggagagaa tttcctgcaa ctgctgacga tcgaaaccgt
    20221 gtttgttcac cattctgtcg atgaactgct gggcattcgg gttattagcg aaatcgccgc
    20281 ccatctgcat cacattgtgc tgcggctcaa gcaggaagcc gccagacggc gttccggtgg
    20341 tcgtttcagt ctcagtaggt tttggcttgc tgctacaggc ggcaagcaac acaaaaaggg
    20401 gaagcaatgt tacataacga cgcttgaaca tgaggggtcc atttaacaga ttcaaccagg
    20461 ggcaagtatg gtaaagcatc acgccccgca caaggaagcg gtagtcactg cccgatacgg
    20521 actttacata actcaactca ttctcctcgc tatcctttta ctcaaacttt caaattaaaa
    20581 tatttatctt tcattttgcg atcaaaataa cactttaaat ctttcaatct gattagatta
    20641 ggttgccgtt tggtaataaa acaataaatc ctgaaggaga gaacaatgat agaaaccatt
    20701 actcatggtg cagagtggtt tatcgggctg ttccaaaagg gcggagaggt gtttaccggg
    20761 atggtgaccg gcattcttcc gctgttgatt agcctgctgg ttatcatgaa cgcactgatt
    20821 aattttatcg gtcagcatcg tattgaacgt tttgctcaac gttgcgccgg taaccctgtt
    20881 tcccgttacc tactgttacc gtgcattggc acgtttgtct tttgcaatcc gatgacctta
    20941 agcctgggtc gctttatgcc ggaaaagtac aaacccagct actacgcggc ggcctcttat
    21001 agctgccact caatgaatgg cctcttcccc catatcaacc ctggcgaact gtttgttatc
    21061 ttggcattgc cagcggtctg acaacgctga acctgccact tggcccactg gcggtgagct
    21121 atctgctggt tggtctggtc accaacttct tccgcggctg ggtgaccgat ctgaccaccg
    21181 ctatttttga gaaaaagatg ggcattcaac ttgaacaaaa agttcatctg gcaggagcaa
    21241 catcatgacg catattcgga tcgaaaaagg aacggggggc tggggcggcc cgcttgagct
    21301 ggaaaccacg ccgggcaaaa aaatcgtcta tatcaccccg ccggtacccg gcctgcgatt
    21361 gtcgacaaac tggcacagct tactggctgg caggctattg acggatttaa agaaggtgaa
    21421 cccgcggagg cggaaattgg tgtcgcggta atcgactgtg gcggcacatt acgctgcggc
    21481 atttatccga aacggcgtat tcccaccatt aatatccact cgacgggcaa gtccggtcca
    21541 ctggcgcagt acattgtgga agatatttat gtctctggcg taaaagaaga aaacatcact
    21601 gtagtgggtg atgcgacacc acaaccctct tccgtgggcc gtgactatga caccagcaag
    21661 aaaatcaccg aacaaagcga tggtttactg gcgaaggtgg gaatgggtat gggttctgcc
    21721 gttgccgtgt tgtttcaatc tggtcgtgac accatcgaca ctgtattaaa aaccattctt
    21781 ccgtttatgg cattcgtctc ggcgctcatt ggcatcatta tggcttctgg ccttggtgac
    21841 tggattgccc acggccttgc tccgctggcg agccatccac tgggtctggt catgctggcg
    21901 ctcatctgct ccttcccgct gctttcacct ttcctcggcc caggcgcagt tatcgcacag
    21961 gttatcggcg tattgattgg cgtgcagatt ggtctcggca atattccgcc gcatctggct
    22021 ttacctgcac tgtttgccat caacgcgcag gcggcctgcg acttcattcc ggtcggtttg
    22081 tcgctggcgg aagcccgtca ggacacggtt cgcgtcggtg tcccttctgt actggtgagc
    22141 cgctttttaa ccggcgcgcc gactgtactg atcgcctggt ttgtctccgg ttttatctat
    22201 caatagaggc tgaaacatga ccgttattta tcagaccacc atcacccgta tcggcgcgag
    22261 tgccactgac gccctcagcg accagatgct catcaccttt cgtgaaggcg cgccagcgga
    22321 cctcgaagag tattgcttca ttcattgcca cggcgagttg aaaggtgcac tccatcccgg
    22381 tttgcaattt acgctcgggc agcatcgcta tccggtgacc gctgttggca gcgtggcgga
    22441 agacaacctt cgcgaactgg gtcatgtcac cctgcgcttc gatggtttaa acgaagcgga
    22501 atttccgggc actgtccatg tggcaggccc tgtacccgac gatatcgcgc cgggatcggt
    22561 tttgaagttt gaatctgtta aggagtaaaa aatgaatcag gttgccgttg tcatcggtgg
    22621 tgggcaaacc ttaggcgcgt tcctgtgcca cggtctggct gccgaggggt atcgcgtcgc
    22681 ggttgtcgat attcagagcg acaaagccgc aaatgtggca caagaaatta acgccgaata
    22741 tggtgaagga acagcgtatg gttttggtgc tgacgccact agcgagcaaa gcgttctggc
    22801 gctctctcgt ggggtagatg aaatctttgt tcgtgtggat ttactggtct acagcgccgg
    22861 aattgcaaaa gcagccttta tcagcgactt ccagctcggc gattttgacc gttcgctaca
    22921 ggtgaatctg gtgggttatt tcctgtgtgc gcgtgaattt tcgcgtttga tgatccgcga
    22981 tgggattcag ggacgcatta ttcagatcaa ctcgaaatcc ggcaaagtgg gcagcaaaca
    23041 caattctggt tatagcgccg cgaaatttgg tggcgtaggg ctgactcaat cactggcgct
    23101 ggatctggcg gagtacggca ttacggtgca ttcactgatg ctcggtaacc tgctgaaatc
    23161 gccgatgttc cagtcgctgt tgccacaata tgccaccaag ctgggtatca aaccggatca
    23221 agtcgagcag tattacatcg acaaagtacc gctcaaacgc ggctgcgatt atcaggatgt
    23281 gctgaatatg ctgctgttct acgccagtcc taaggcgtcg tactgtaccg gacagtcgat
    23341 caatgtcacc ggcggtcagg tgatgttctg atcaacagcg gagatccgtt aaggatctcc
    23401 gtgagactaa tagaatgcct gatgcgctac gctcatcagg catacaggac ttccgccact
    23461 acattaagga aaagttatgg tatccgcact catcactgtc gccgttatcg cctggtgtgc
    23521 gcaactggcc ttaggcggct ggcaaatttc tcgttttaac cgtgccttcg acacactatg
    23581 ccagcaaggg cgggttggcg tgggccgttc cagcgggcgc tttaaaccgc gggtcgtggt
    23641 cgccatcgcc ctggacgatc agcagcgcat cgccgacacc ttgtttatga aaggactgac
    23701 cgtcttcgcc cgaccgcaaa aaattcccgc aattaccggt atgcatgcgg gtgatttaca
    23761 gcccgatgtg atctttcccc atgatccact atcacagaat gctctatcat tggcgcttaa
    23821 actgaaacgt ggataatttc gttgtgaatg ttacttgctt gcgaagttat cattttgaaa
    23881 cctaaatcag gtaatcacgc ccatgaaacc tcgtcagcgt caagccgcca ttctggagta
    23941 tctgcaaaag cagggaaaat gctcggttga agaattggcg caatattttg acaccacagg
    24001 cacaactatt cgcaaagatc tggtcattct ggaacatgcc ggaaccgtca ttcgtactta
    24061 tggcggagtg gtgttgaata aagaggaatc cgatccgcct atcgatcata aaacactcat
    24121 caacacccac aagaaagagc tgatagcaga agctgccgtt ggttttattc atgatggcga
    24181 ttcgatcatt cttgatgctg gcagtaccgt tttgcagatg gtgcctctgc tctcgcgctt
    24241 taataacatc acggtgatga ccaacagcct gcatatcgtc aatgcgttat ctgaactgga
    24301 taatgaacaa actatcctga tgccaggcgg aacgtttcgt aaaaaatcgg cctcatttca
    24361 cgggcagctg gcagagaatg ccttcgagca tttcaccttc gacaaattat tcatgggtac
    24421 cgacggcatc gatctcaatg cgggcgtaac caccttcaac gaggtctata ccgtcagtaa
    24481 ggcaatgtgc aatgccgcgc gcgaagtgat tttgatggcg gactcatcaa agtttggccg
    24541 taaaagcccc aacgtagttt gcagtcttga aagcgtcgat aagctgatta ccgacgcaga
    24601 tatcgatccg gcgtttcgtc aggcgctgga agagaaaggg atcgatgtga tcataaccgg
    24661 agagagcaat gagtgaagca ctactgaacg cgggacgtca gacgttaatg ctggaattgc
    24721 aggaagcaag ccgtttaccg gaacgtctgg gcgatgattt tgttcgcgcc gccaatatca
    24781 tcctgcactg cgaaggcaaa gtggtggttt cgggaattgg caaatcgggc cacattggta
    24841 agaaaatcgc cgcaacgctt gccagtaccg gcacaccggc tttttttgtc catccggcag
    24901 aagcgctgca tggcgatctg gggatgatcg aaagccgcga tgtgatgctg tttatctctt
    24961 actccggtgg ggcgaaggaa ctggatctga ttattccgcg tctggaagac aaatctatcg
    25021 cgctgctggc gatgaccggc aaaccgacgt caccgctggg cctggcggcg aaagcggtgc
    25081 tggatatctc cgtagaacgc gaagcctgcc cgatgcacct tgcgccgacc tccagcaccg
    25141 tcaataccct gatgatgggt gacgcgctgg cgatggcggt catgcaggcg cgcggattta
    25201 atgaagaaga ttttgcccgc tcccaccctg ctggagcact gggcgctcgc ttgctgaata
    25261 aagtgcatca tctgatgcgc cgtgacgatg ccatcccaca ggtggcgtta accgccagcg
    25321 tgatggatgc tatgctggaa ctcagccgca ccggtctggg gctggtggcg gtatgtgacg
    25381 ctcaacaaca ggtacaaggc gtctttaccg acggcgattt acgtcgctgg ctggttggcg
    25441 gcggcgcact cacaacgcca gtcaatgaag cgatgacggt cggcggcacc acgttgcaat
    25501 cgcaaagtcg cgccatcgac gccaaagaga tcctgatgaa gcgcaaaatc actgccgcac
    25561 cggtggtgga tgaaaacggc aaactcaccg gcgcaataaa cctgcaggat ttctatcagg
    25621 ccgggattat ttaatccttc aatcccagac gtttcgccag ccgatgcagg ttggcgacgt
    25681 cggtttccag catccgcgcg caggcagccc agttgtgatg attttgtgcc agtgcctgac
    25741 gaatagtttc acgctggaac gcttctgtcg cttcacgcag gttttgctta acaacgggca
    25801 ccgccgccac ttctggcggc ggcaacgtca cctcaggaaa agcaaaatgt tgcgcctcaa
    25861 gaatcacttc atcgccgctg cgggtggctc tcgccagcac taccgcccga tgaatagcat
    25921 gttccagttc gcgcacgttt cccggaaaac tgtagtgttg cagtaaattt cgcgccccgg
    25981 cacttaatac cacgcgggag agcccctgcc gcaaacgaca ctgctcgcag aaataccccg
    26041 ccagcagaat gacatcatcg ccccgctcac gcagcggcgg caccgaaagt ggaaacacac
    26101 tcaggcgatg aaacaaatcg gcgcggaatc gccctgccag cacctcttcg cgtaagtcgc
    26161 ggttagtcgc cgccagcacg cgcacatcga cccgcaaact acggtcatcg ccaacgcgct
    26221 gaatatcgcc atactgcaac accctcagca gcttggcctg caatgccaac gacaactcgc
    26281 cgatctcatc gagaaacagc gtgccgttat ccgccatttc aaacttccca ctgcggttac
    26341 tgatagcgcc agtaaacgct cctttcacat gcccgaacaa ctcactttcc gccacacttt
    26401 ccggcagtgc ggcacagttg agatagacca gcggattcac cgcccgtggc gaggcctcat
    26461 gaatcgcttt cgccaccagc tccttaccgg ttcccgtctc accgctgatc aggacgttga
    26521 gatcggacgc cgccacaatc tcaatctctt ttttcagttg cgtcatgcct ggcgacagac
    26581 cgatcatctg cgtctgtttc accgcttcaa atggagcggc atcgcccggc agcatattct
    26641 ggctttccag ttgttcaatc agcaacgcat tgcttaacgc tcccgccgcc agcgcggcaa
    26701 tcaggcgtaa ctcttcgtcg ctgaaaacat cgaactgatc gggctgcatc ccgtcgagcg
    26761 tcaatgcgcc gatcaggttt tgcccggcaa acagcggcag accaacgcag gcgtgaacct
    26821 tcagactctc ctgccctgga atcaaaccgt catagggatc gggcaattcg ctgtctgcgg
    26881 gaaagcgcac cacatcaccc gcgcgggcaa tcgcttccag ccgtggatga ccttccagcg
    26941 caaagcgtct accgagtaca tccttcgcca gcccgtcgat ggcaagcgga ataaactgcc
    27001 gcgagtcgta acgtagcaac gcagacgcat cgcactccag cacctgacgc agcgtggtga
    27061 tcaggcgctg aaaacgatcc tgatgaccaa tcccacgctg caattcgatg gcgatattcg
    27121 ccagcacatc aacggaaaaa ctcatctttg cctcactgtc aatttgacta tagatattgt
    27181 catatcgacc attggattga tagtcatttt gactactcat taatgagcat aattttattt
    27241 atagagtaaa aacaatcaga taaaaaactg gcacgcaatc tgcaattagc aagacatctt
    27301 tttagaacac gctgaataaa ttgaggttgc tatgtctatt gtggtgaaaa ataacattca
    27361 ttgggttggt caacgtgact gggaagtacg agattttcac ggcacggaat ataaaacgct
    27421 gcgcggcagc agctacaaca gctacctcat ccgcgaagaa aaaaacgtgc tgatcgacac
    27481 cgtcgaccat aaattcagcc gcgaatttgt gcagaacctg cgtaatgaaa tcgatctggc
    27541 ggatatcgat tacatcgtga ttaaccatgc agaagaggac cacgccgggg cgctgaccga
    27601 actgatggca caaattcccg atacgccgat ctactgtaca gccaacgcta tcgactcgat
    27661 aaatggtcat caccatcatc cggagtggaa ttttaatgtg gtgaaaactg gcgacacgct
    27721 ggatatcggc aacggcaaac agctcatttt tgtcgaaaca ccaatgctgc actggccgga
    27781 cagcatgatg acttacctga caggcgacgc ggtgctgttc agtaacgatg ctttcggtca
    27841 gcactactgc gacgagcatc tgttcaacga tgaagtggat cagacggagc tttttgagca
    27901 gtgccagcgt tactacgcca atatccttac gccgttcagc cgtctggtaa cgccgaaaat
    27961 taccgagatc ctgggcttta acttaccagt cgatatgata gcgacctctc acggcgtggt
    28021 atggcgcgat aacccgacgc aaattgtcga gctgtacctg aaatgggcgg ctgattatca
    28081 ggaagacaga atcaccattt tctacgacac catgtcgaat aacacccgca tgatggctga
    28141 cgctatcgcc caggggattg cggaaaccga cccacgcgtg gcggtgaaaa ttttcaacgt
    28201 cgcccgaagc gataaaaacg aaatcctgac caatgtcttc cgctcaaaag gcgtgctggt
    28261 cggcacttcg acgatgaata acgtgatgat gccgaaaatc gccgggctgg tggagaagat
    28321 gaccggatta cgcttccgta acaaacgcgc cagtgctttc ggctctcacg gctggagcgg
    28381 cggtgcggtg gatcgtcttt ccacgcgcct gcaggatgcg ggtttcgaaa tgtcgcttag
    28441 cctgaaagcg aaatggcgac cagaccagga cgctctggag ttatgccgtg aacacggtcg
    28501 cgaaatcgcc cgtcagtggg cgctcgcgcc gctgccgcag agcacggtga atacggtagt
    28561 taaagaagaa acctctgcca ccacgacggc tgacctcggc ccacggatgc agtgcagcgt
    28621 ctgccagtgg atttacgatc cggcaaaagg cgagccaatg caggacgttg cgccaggaac
    28681 gccgtggagt gaagtcccgg ataacttcct gtgcccggaa tgctccctcg gcaaagacgt
    28741 ctttgacgaa ctggcatcgg aggcaaaatg agcaacggca ttgtgatcat tggttcgggc
    28801 ttcgccgccc gccaactggt gaaaaatatt cgcaaacagg acgccagtat tccattaacc
    28861 ctgattgccg ccgacagtat ggatgagtac aacaaacctg acctcagcca tgttatcagt
    28921 caggggcaac gtgccgacga ccttacccgc cagacggcgg gtgaatttgc cgagcagttt
    28981 aatctgctcc tgtttccgca aacctgggtg acggatatcg atgccgaagc ccgtgtggtg
    29041 aaaagccaga ataatcagtg gcaatacgac aagctggtgc tggcaaccgg tgccagcgcc
    29101 tttgtcccgc cagtgcctgg gcgtgagtta atgctgacgt taaatagtca gcaagagtat
    29161 cgcgcctgtg aaacgcaact gcgggatgcc cgacgcgtgt tgattgttgg cggtggtttg
    29221 attggtagcg aactggcgat ggatttttgt cgtgcaggca aaatggttac gctaatcgac
    29281 aacgctgcca gtattctggc gtcgttaatg ccaccggaag taagcagtcg cttgcagcat
    29341 cggttgacgg agatgggcgt tcatctgctg ttgaaatctc agttgcaggg gctggaaaaa
    29401 acggattctg gcattctggc aacgctggac caccagcgca gcatcgaagt ggatgcggta
    29461 attgccgcca ccggactgcg cccggaaacc gccctggcac gacgcgccgg gctgacgatt
    29521 aatcgcggcg tttgcgtcga tagttatctg caaaccagta atgccgatat ttacgcgctg
    29581 ggcgattgcg cggaaattaa cggtcaggta ttgccgttcc tgcagccgat tcaacttagc
    29641 gcgatggtgc tggcaaaaaa tcttctcggc aataacacgc cgctgaaact accggcgatg
    29701 ctggtgaaaa tcaaaacgcc ggaattaccg ctgcatctgg caggcgaaac ccagcgtcag
    29761 gatttacgct ggcagattaa taccgaacgc cagggaatgg tggcgcgcgg tgttgacgat
    29821 gcagaccaac ttcgcgcctt tgtggtcagt gaggatcgga tgaaagaggc atttggattg
    29881 ttgaaaacgt tgccggtgta ggtgggctac tgtgcctaaa atgtcggatg cgacgctggc
    29941 gcgtcttatc cgacctacgg ggacgcatgt gtaggccgga taaggcgttt acgccgcatc
    30001 cggcaatggt gtccaaatgc aacacgtttt atccgttctg gacttcaccc gctaaccaac
    30061 gcgccgcagc aataaccccc tgccccagag acaaaccgcc atcacccgcc ggtaaactct
    30121 gtggaaacag caatgtgaaa tcagcgagat aatgcgccag acgtgcacgc agcaaacggt
    30181 tatgaataac cccgccgcta aataccagcg tagtgatacc acgcatcgtg gcctgctcac
    30241 gcatcaacgc ggcaaaaccc tgcgccagcg catcatgaaa cgcccacgcg cgttgattaa
    30301 ccggtgcctg ccagttcagc cactgctgcc agaaagtggc gagatccagt tgattgtcca
    30361 cccgcggcat cgtcaccgga tgcgtcactc cgtcgcatga ggctgctagc gcctccagag
    30421 cacaagccgc ttcaccttca taacttaacg tggctggcgc acagcccagt gccgccgcca
    30481 cagcatcgaa caaacgccca cacgatgacg ccagcggcgc gttaattcca cgctcaatgg
    30541 cccgcgccag tacgctccag ttttgctgtt gcacacttgc cgtttcaggg taattctgcc
    30601 actccggcac aaagcgcagg cactgcgcca gcaggttacg ccacggctgc ttcgccgcca
    30661 aatcgccacc cggaagcgcc actgcaggca agccgcccag gtgctcacat tcgcgatagt
    30721 tcacccgcag gcactcgccg ccccacaaag cgccgttctc ccccataccg ataccgtcga
    30781 gcgtcaaagc aatgacatca ccgccatcca gcggccactg atgctctgcc agacacgccg
    30841 ctgcgtgggc atgatgatgc agcaccgttt gcgtcggcag attcatttcg ctcgcccact
    30901 ggcaggagac atagcccgga tgtgcgtcat gcacaacgta ttgcggagtg aaattgtaga
    30961 tgttttgcat caggcgtaac gcttcgcgcc actgcgtctg gatgccatcg tcacttaaat
    31021 cgcccagatg ctggctcaac accacttgtt caccgcgcac caggcagaag gtatttttca
    31081 gatccgcgcc gagacacagt acaggcggaa catttttaaa gcccggaggc aaagccagcg
    31141 catccggcac atacccccgc gaacggcgca gcatttcgcc gctttcgcgc accaccgaat
    31201 catccatccg ctgcacaatg tcgcggttgt gtattaagaa tccgtcggca atgccctgca
    31261 aatcctccag cgcctgttcg ttgctgatag ccggtggttt accgctcagg ttgccagagg
    31321 tcatcaccag cgggcattgt agttcctgta acagcaaatg ctgaagcgga ttcgcaggca
    31381 acattacccc gacttcgtta aggccagggg cgatatcatc acaaagctca ggaacgtatt
    31441 ttttatccac cagcacaatc ggcgcggcgg gcgtggtaag caactggcgc gcagcgtctg
    31501 gtaaaccgtc agccactggc aacatgactg ccagcggttt cgccgggcga tgtttgcgcg
    31561 cccgaagtgt cgccaccgcg ttactgttac gtgcatcgca ggcaagatga aatccgccaa
    31621 tccctttgat ggcgacaatg ttgcccattt ttaactgtgc gatagccgcc tgtaatgccg
    31681 cctcttgttc tgcatgttca ccatgactta cccattcaag atacgggcca cactccgggc
    31741 aggccaccgg ctgggcgtgg aagcgacgat cgagcgggtc gcggtactct ttatcacagg
    31801 ctggacacag cggaaacgcc gccatcacgg taaacgggcg atcgtaaggc atggcgcgaa
    31861 taatggtgaa gcgcgggccg cagtgggtac agttgataaa tggataacga taacgccgtt
    31921 cgcctggggt attcatttcg gcaaggcaag cagggcaagt ggcggcatcg ggaacaattt
    31981 gcgtattcat ggcgccgccc gcgctctggc ggatggtgaa ctcagtgggc agttgtgacc
    32041 agataaacgg ttcacgctca acgctatcaa tacgcgccag cggcgggcag tgctgatgca
    32101 attgaacaag aaacgtttcc gggtcttcca gcagccggac ttctacgcca tcgccgtcat
    32161 tacagacatc gccgtgaaga tttaattgct gtgccagctg ccagacaaac ggacgaaaac
    32221 cgacgccctg cactttgcct cgaatacgca gttggacacc gcaagatgtg ttttttgcca
    32281 ttgagttatt cccgccatca tgaattgcgt aacccgtcca gccggacacg acagcgtcgc
    32341 atccggcagt cacaggtcgg cgataccgcc cgctccgtat tctacgaata tttccgggaa
    32401 ttcctttgat gccagaacag ttctgtaaga tttttagaac atcagcgccg tacggcggcg
    32461 tttttctgcg ctcagttgtt caagtttatt gcgatcgaca caaattagcg catgggtcgg
    32521 gcaagccgcc atacacgccg ggccgtcttc acgatggttg cacaggtcgc atttattggc
    32581 ttcggctttg tcagcccgta cattcagccc tgcgccgctg ttgcgaatca ccggacgtac
    32641 caccacttcc atcgcgccat acgggcaagc cacaacgcag gttttgcaac caatgcaacg
    32701 ttcctgcatc acatgaacaa accctttatc acggctgata gcaccattcg ggcagacgtt
    32761 agcgcacggt gcatcttcac actgacggca aactgtcgcc gtggaaatgt tcacaccttt
    32821 aatgacatgg atacgcggta aaaatgtttc cggggtcagc gatgcacagt cctgattttc
    32881 ctgatgagaa accacgcacg ccacttcaca ggtacggcaa ccaatacatt tactcgcgtc
    32941 agcaatgatg aaacggttca tcaaattctc cagcaatgac agttaatgcg ccgatacatt
    33001 cacaaatcat gccagttttt aatttgctgt tatttaagga aattaatttc tgtaatgcag
    33061 gaaaaaagat gtcatcgaca ctagtgacga tgacatgtga tgacaatgtt tatcgcgaaa
    33121 gagcaatgag tgagtcgcgg cggatcagtt ttccgctgaa ggttttcggc ggtgagaaat
    33181 ccccgccatc gagcataaaa atcagccgtc caataatttc ctgaatcatc tcagtcaccg
    33241 gaatttttac gctggagagc gccggaacga tatagggggc aattgcgata tcatcgaatc
    33301 cgataactga cacctgctct ggcaccgcta cgccgcgctt gtgtaacgct ttcatcgcac
    33361 ctatcgccat atcgtcgtta ctggcaacta acgcgctaaa tttagcccca cgttcgagca
    33421 acgtttctac cccttcggcc ccgctggcgg gcgtccattt accgttagcg ataagttttt
    33481 cattgagcgc aataccatgc tgcgccagcg cgtctttata tccggcaaga cgttcaacgc
    33541 tggtggggga atccatcgag ccggtaagaa aagcaatctc ctgatgcccg gcgtttatca
    33601 actctgccac ggcgttaaag ctggtctgtt tatgatcgca ccagacgcta tggctgctat
    33661 ttttgcgcag gcggcgatta agcaccatta tcggctgact gtgcgcgtca atgatgtcat
    33721 cgatctcatc cacacttaaa aaacgcgggt aaatcataat ggcgtcgcag cgcaaatcca
    33781 gcagatactg aatcgcctgg cgctcttctt cggcgctgtg tttaccatct gccaatagca
    33841 actgccgccc tttctcttcc gccattcgcg cggcatgaaa cagtaattca ctaaaataaa
    33901 tgccgtggta aagcgtgtta gtcactacca gccccagcgt ctgggtactt ttcgccgaca
    33961 gattgcgcgc cagcaagttt ggacggtaac cgctctcttc taccgcctga aacacgcgat
    34021 ctttagtctc ctggctgacg tagccattac ctgaaagcac gcgggaaacg gtcgcttttg
    34081 aaaccccggc gcgcttcgcc acttccagca tcgtcgtcat cattttcatc cctttacacg
    34141 caatcaacgc agtgtactgc accgtttacc gattgtcctt gcgcaatcgg cgggaaaaat
    34201 attcaggtga ccggtttcac aaatataaaa aatgaacaat taactctctt gcttatttgg
    34261 ttacaactat tcatgatttt gtgaaaccgg tttcttaatt ccgtttcagc atcggcattt
    34321 ttccgtcacg tcgactgata acaactacat cttccctact gataacagga taaaatccga
    34381 tggccaaaaa ttatgcggcg ctggcacgct cggtgatagc ggcactgggc ggcgttgata
    34441 acatctcggc ggtcacgcac tgtatgacgc gtttgcgctt tgttatcaaa gatgatgcgc
    34501 ttatcgacag cccgacgtta aaaaccatcc ccggcgtgct cggcgtggta cgtagtgaca
    34561 accagtgtca ggtgattatt ggcaataccg tttcacaagc ctttcaggaa gtcgtcagcc
    34621 tgctgccggg agatatgcag cccgccctgc ccgtgggtaa acccaaactc acgctgcgtc
    34681 gcattggtgc ggggatcctc gatgcgctga tcggcaccat gtcaccgctg atcccggcga
    34741 ttatcggcgg atcgatggtc aaactgctgg cgatgatcct cgagatgagc ggcgtgctga
    34801 caaaaggatc gccgacctta accattctga atgtgattgg tgacggtgct ttcttcttcc
    34861 tgccgctgat ggtcgcggct tcggccgcca tcaaatttaa aaccaatatg tcgctggcga
    34921 ttgccatcgc gggtgtgctg gtacatccga gttttattga actgatggcg aaagcggccc
    34981 agggtgaaca tgtcgaattt gccctgattc cagtcaccgc ggtgaaatac acctacacag
    35041 tgatcccggc gctggtgatg acctggtgcc tgtcatatat cgaacgctgg gtggatcgca
    35101 ttacaccggc ggtgaccaaa aacttcctca agccaatgct gattgtgttg attgccgcac
    35161 cgctggcaat cctgctgatt ggcccgattg gtatctggat cggtagcgcc atttcggcgc
    35221 tggtttacac cattcatggt tatctgggct ggctttcagt cgccattatg ggcgcgctgt
    35281 ggcctctgct ggtaatgacc gggatgcacc gcgtctttac gctaaccatc attcagacca
    35341 ttgccgaaac cggcaaagaa gggatggtca tgccgtcaga gatcggcgct aacctgtcgc
    35401 tgggcggttc atcactggcg gtggcgtgga aaacgaaaaa ctcggaactg cgccagactg
    35461 cgctggctgc ggcggcatca gccattatgg cggggatttc cgaaccggcg ttatacggcg
    35521 tggcgatccg cctgaaacgt ccgcttatcg ccagtcttat cagcggtttt atttgcggcg
    35581 cggttgccgg tatggcgggg cttgccagcc actcaatggc agcgccgggg ctatttacca
    35641 gcgtgcagtt cttcgatccg gcgaatccaa tgagcatcgt ctgggtgttc gcggtcatgg
    35701 cgctggcggt ggtgctgtcg tttatcctca cactgttgct cggctttgag gatattcctg
    35761 ttgaggaagc ggctgccgag gcgcgaaagc atcagagcgc acaaccgacc gtcgccaaag
    35821 aagtaagtct taattgagga tgaaaatgtc agtatttcca gaaggttttt tatggggcgg
    35881 cgcgcttgcc gccaaccagt ctgaaggtgc gttccgtgaa ggtggcaaag gactgacaac
    35941 ggtcgatatg atcccacacg gcgagcatcg aatggcggtg aaactggggc tggaaaaacg
    36001 ttttcagttg cgcgatgacg agttttatcc cagccatgag gcgacgggtt tttatcatcg
    36061 ttataaagaa gatatcgccc tgatggcaga gatgggattc aaagtgttcc gtacctcaat
    36121 tgcctggagc cgccccttcc cgcagggtga tgaactgacg cccaaccagc agggcattgc
    36181 tttttaccgt tctgtctttg aagagtgtaa aaagtacggt atcgaaccgc tggtcacatt
    36241 gtgccacttc gatgtgccga tgcagctggt caccgaatat ggctcctggc gtaaccgcaa
    36301 gctggtggag tttttcagcc gctacgccag gacctgcttt gaagcatttg atggtctggt
    36361 gaaatactgg ctaaccttca atgaaatcaa cattatgttg catagcccgt tctccggcgc
    36421 ggggctggtg tttgaagaag gtgaaaatca ggatcaggtg aaatatcagg ccgcgcatca
    36481 ccagctggtt gccagtgcgc tagccaccaa aatcgcccat gaggttaacc cgcaaaatca
    36541 ggtgggctgt atgctggcgg gcggtaactt ctacccttac agttgcaagc cggaagatgt
    36601 ctgggcggcg ctggagaaag atcgggaaaa cctgtttttt atcgatgtgc aggcgcgggg
    36661 cgcgtatccg gcttactctg ctcgcgtatt ccgcgaaaaa ggggtaacca tcgacaaagc
    36721 accgggcgat gatgagatcc tgaaaaacac cgtcgatttt gtctctttca gctattacgc
    36781 ctcgcgctgc gcctcggcgg agatgaacac caacaatagc agtgcggcga acgtagtgaa
    36841 atcgctgcgt aacccgtatc tacaggtgag cgactggggc tggggaattg atccactcgg
    36901 tctgcgtatc accatgaaca tgatgtacga ccgctatcag aagccgctgt ttctggtgga
    36961 aaacggcctc ggcgcaaaag atgaacttgc tgccaatggc gagattaacg atgactatcg
    37021 catcagctat ttacgcgaac atatccgcgc aatgggcgaa gcgattgcag atggcattcc
    37081 gctgatgggc tacaccacat ggggctgtat tgatttagtt tccgcctcta cgggtgaaat
    37141 gagcaaacgc tacggttttg tctttgttga ccgtgacgac gcaggcaacg gcacgctgac
    37201 gcgcacacgt aagaaatcgt tctggtggta taaaaaagtg attgccagta atggggaaga
    37261 tttagagtag gtaacctact gtcgcactaa ccaggaaggc ccgataagcg ttgcgcatcg
    37321 ggcaatctgg ggtttgctat cagttctgat tccctcatca agaacatccc tgtcctgatt
    37381 acttattgaa aaactactct tcttccaccg ctaactgcgc gaagccgccg ttcccttccc
    37441 agccttccag tcgttgataa acggtttcta ccgcatcttt aatcggctgg gtcatcgggt
    37501 agtaaaagcc gacgatatcc ggctggatgc cgaggaaaat cacttcgcca atatcttctt
    37561 tcaactgatc gataaggtag ttcaacggca tgttatgggt agtcatcata aacatctcgg
    37621 cgatatcatc cgggtcgatg atgcggattt cgccggggtt tagccccata tccgtggcgt
    37681 cgacaatcag cagccgtgtc gggcgcagtt cgcggatagc gacaatgtcg ttttccggtg
    37741 cgctaccgcc gtcaatcacc acccagttac ctttcggcgc ggcggcgcac ttttccgcca
    37801 gcagcggacc tgcgccatca tcacccatca tgctattgcc aacacagagt aaaacgtcag
    37861 tcacgcagtc tcctcaccat caaatagatg gcgttttcct gatgaatatc atgcagcata
    37921 cttaacagca ttgtactcca ttcctgctgc tgcgaagttt gcgctgcccg ggctttatcg
    37981 aatgcattgg ccagcatcgg cacatggtta atgtcgatga cgatctcacc atatttcggc
    38041 acaccttcca ttttgcggcg ggcttcgctg cccgcctcca gagtggcaat ccatgccaga
    38101 tattcatccc acgggcaggt gagcgccgct tccaggcaat cgataacccc aaggtggtga
    38161 ccaatcgcca ggctgtaata gaccacctgc tgcgcctcgg cgggcgtggc atcgttctca
    38221 tcaataaatt tacggctcag ttgactgaac accacctttt cactcatcgg atacgcgcct
    38281 cttcaacaac atgattcaga tggctgacaa tctcgttcag acgcggatcg ttttccgctt
    38341 ccagccagcg tgcaacctgt tcttcgccct gccctaactg tgtaaggtaa tcatcggcaa
    38401 tctgacgacc gtaacgataa cccgccagtc gacgtgcttc gcgatccact ttcacacgca
    38461 gcggctgcac catatcgcca tgcaggatct ccgccggttg ttcatcctgc tcacccggcc
    38521 cacgggcgtg aattttctgc tccagcaggc cgagcgccat tgcaaagccg tacagcgtgg
    38581 cggctggcgt tggcgggcag ccagggatat aaacatccac cgggacgatt ttatccgtac
    38641 cgccccacac gcagtagaga tcgtggaaga tcccgccgct gttaccgcag gcaccgtagg
    38701 agatacaaat tttcgggtcc ggcgcggact gccacgcacg cagcgcaggg gatcgcattg
    38761 cacgggtgac cgcgccggta aacagtaaaa tatccgcatg acgcggtgaa ggaacgactt
    38821 taatgccgaa gcgttctgca tcgaacagcg gcgacaaagt ggcgaaaatt tcgatttcgc
    38881 agccgttgca gccgccgcag tccacgcggt aaacgtaggc agaacgtttg atttttttca
    38941 gtaacgacgc cttcatgctg gcgatggatt catccaccgt catcgggacc ggaatgccgt
    39001 tggcgtcacg ggggcctaat aaattgctca tcagatggcc tctttcatat ggcgagtcag
    39061 ttcaatacgg tcggacggca ccaggcattt ctggcgctta cattccgggc aagtctcaaa
    39121 gctttcgcgg tggttttccg cgcggctgtc gccattgtgc ttaagcagcg caatggcgta
    39181 gtcgatctct ttctggacgg cgaaaggacg attgcagacg cggcagttgc acagcgcgaa
    39241 gcgggactgt tgcaggaagt cttctttctt ccacaccgcc agttcgtact cttgcgacag
    39301 tttgattgcc gccgtcgggc agacttcttc gcagcgtccg cagaagatgc agcgcccgag
    39361 attgaactcc cacgccagct caccggtggc gaggtcagtt tcaaccgtta aggcgtttga
    39421 cgggcaggca ttgacgcagg ccgcgcagcc gatgcactgc tgcgggttct gctctggctt
    39481 accacggaag tttttatcaa ccgcaatcgg ttccagcgga taagacgagg tcgccgtgcc
    39541 ggttttgatg acttttttga taaaggtaaa catggcgatt ccttatttca gcggcgagtt
    39601 tttacgctca atgctgtaac gctcgagttc tttgtacggc accactttgc tctttttctt
    39661 acgcacatcg accacggtca tgcggtcggt acaggagtag caagggtcca gactaccgat
    39721 aatcagcggc gcatcagaaa cggtgttgcc gcgcagcatg tagcgcaggg tcggccagtt
    39781 cgcgtaggtc gcggcacggc agcgccagcg gtacagcttc tggttgtcgc cggtcatgct
    39841 ccagtggata tcatcgccgc gcggcgcttc ggcaaagccc agcgcaaaac ggtgcggaat
    39901 gtaggtaaag ccttccacca tcagcgggcc gcccggcagg ttatccagac cgtagtcgat
    39961 catgttcagc gcggtataga cttcgttaat acgcactttc agacgcgaaa taacgtcgca
    40021 gccctgctcg ctgtggactt ccattggcag caggccataa ccaacaaacg ggtgatcggc
    40081 gcgggtatcg cgggcgtgac cgctggcacg gaccatcggg ccgacgttac tgaagtcgcg
    40141 agcgatttcc gggtccagac gaccaatgcc gacagtgcgc tgttccatgt tcggcgtact
    40201 cagcagcaca tccaccagct cctgcacttc acgacgcatc tgttgcgcca gttgacgggt
    40261 ctggatcatg tcatctttca gcagatcgcg acgaatcccg ccgatcaggt tcaggccgta
    40321 ggttttacgc gcaccggtaa ggatctctgc cattttcatg gaggtttcac gcacgcggaa
    40381 gaactgcata aagccggagt caaagccggt aaagtgacag gcgaggccga ggttgagcag
    40441 atgcgagtgc agacgttcta cctccagcag aatggcgcgg atcatctgcg cacgttctgg
    40501 cacctgaata cccatcgcgt tttccaccga cgtggtgtag gcggtgctgt gagcaaaacc
    40561 gcagatcccg cacacacggt cagagaggaa ggtcacttcg ttataaccca tgcgggtttc
    40621 tgccagtttt tccatgccgc gatggacata gaacaggcgg tagtcggcgt cgataatgtt
    40681 ttcgccatcg acgaacagac ggaagtggcc cggttcgtca gaagtgacgt gcagcggacc
    40741 aatcggcacg acgttgtttt tcttgtcgcc cagttcgttg atgaactcgt aggtttcagc
    40801 atcggtggtc ggtgccggac gctgacgata atccatgctg tctttacgca gcggataaag
    40861 ttcatccggc cagtcatccg gcagcaccag acgacgttca tccggcaaac caaccggaat
    40921 caaaccgtac atatcacgta cttcacgctc gccccacacc gcagccggaa cgcgcggcgt
    40981 cacggaagga tactccggtt tgttggcatc aacttcgacg cgaaccgtta cccagcactt
    41041 agtccccttc tccatcgaca gcacgtagta aacggcgtaa tgaccattca gtttgcgttc
    41101 gtcgttacca aaaagcaccg acagccagcc cccctgcttg tagtagagaa actccaccac
    41161 ttccggcaga tagttcacct ttacggtgac agtcagctga tctttggtct gccaggcgtg
    41221 gtccagcacg acgcccggaa atgcctcatt cagcgcggcg agataatgtt gacctaattt
    41281 ttcttcagac atgctcaaac tctctttaat cacgccgcca gcaaggagac gaacgctaaa
    41341 aatgcaaagc caaacccagc ccaggtaatg cgcggagtaa tatcaagacg cagacgggcc
    41401 atgctgtttt cgaacagcgc gataaccagg acgccgacca ccagtttgac gatggcaatc
    41461 accaacgcca gcagcagccc accgacggtg aaggtttcca tttgtcccca cggaataaac
    41521 accccgacga acatctgcag caccaccaac tgtttcaggc tgatacccca tttcatgacg
    41581 ccaaagccgc tgccgctgta ctcagagagc ggaccttcct gcaactcctg ctcggcttcc
    41641 gccaggtcga acggcagttt gcccatttcg ataaaagtgg cgaacgcaca ggcacaaagc
    41701 gccagcacca gcgggatgct ctggctcagc ggccagtgat aaacggtgtc ggtgatgttg
    41761 ctgatgttgg tggaactggc aacctgtgcg gcaacccaca gaccaagcag cagcatcggt
    41821 tcgaccagca cgccaagcat cgcttcacgg ctcgcgccga tagcggtaaa cggactaccg
    41881 gtatccagac cagaaatggc aaagaagaaa cgcgcgatgg caaagagata cagtaaggtg
    41941 atcaaatcac ccagttgcgg cagcggagaa ccgacggtca ccaccggcag cgcagtagcg
    42001 atagtcagca tgacgcccac catcacatac ggcgtcaggc ggaacaccca gccggaggca
    42061 tccgggccaa cgctctggcg acccagcagt ttgataatgt cgcgatactc ctgcaacacg
    42121 cccggcccgc gacggttatg caagcgggcg cgcgccacgc gggtaatacc ggagagcagc
    42181 ggcgcaacgg caaatagcac cagcgcctga attaacggat ataaaacact cattctcagg
    42241 ctcctcgtga aacaataatc accaccagca ccgccagttc aaccagcgcc atccggcgga
    42301 acagcaacgc actgccttcg cactgccagc ccggcaccag cgacaccgga ttcagccatt
    42361 tgcgcagttt cagcaccggc gcaaacgcct gtttcaccgg catggcaaaa ccgtgggcgg
    42421 taattaccat tgatttttca tggtcgtagc cgcacaccca ggccgcgccg cgggaacgcg
    42481 atggcaaacg atcgcctttg caaatcgcca taatgatgaa tggcagcagc gggcaggcaa
    42541 tcagcagcaa cgtgatcatc ggttgagaaa cggtggtgtt agcaggttcc agcggcagag
    42601 gtacagcagc agagagcatc ggcagcagcc acggcgcagc aacaccgcca attacgcagc
    42661 aaatcgccag tgccactacg cttacgctca tcaggagcgg tgcacaggtg gcgttttcgg
    42721 cttctttggt acgcggcgcg ccgaggaacg tgacgccata gactttcgcc atacacatca
    42781 ccgccagcgc accggtaatt gccagaccca cagcgagcag cggccccagc agacgggcaa
    42841 caaacgcgcc actattgctc agtttgaaga aggattgata gataacccat tccccggcaa
    42901 aaccgttcag cggcggcagt gcagccattg ccatcagccc gactaacata gcgatggaga
    42961 taaccggcat tttcttgcca ataccaccga gtttttcgat atcgcgatga ccggtacgga
    43021 accagacgct ccccgccccg aggaacagta cgcttttgaa caggctatgg ttaagcagat
    43081 ggtacagacc accgaccagg ccaagagcaa tcagcgccgg ttgttcgagc gcgatacccg
    43141 ttacgccagc gcccagcccc agcaggataa tgccgatatt ttccagggtg tggtaagcca
    43201 gtagacgctg gatattatgt tccatcaacg catacagacc gccgacaaac gcggtgatca
    43261 taccgagcac cagcagcgcg atcccccacc acagcggtgc attaccgccc agcagtgaca
    43321 gggttaaaat gcccagcagg ccaattttca tgactaccgt agaaaacaac gcggcagctg
    43381 gcgcagaggc gttcgcatgt gcctgcggca cccagccgtg cagcggaata atcccggcca
    43441 gcaggccaaa gccaatcaca ctgagcagcc agatatcgga accgagcggc agctgttgca
    43501 tacgcatatc cagcaggcgc agatccagcg tgccgtaacg ctgccacagc agccagcaag
    43561 caatcgccag cagcagagtg ccaagacgcc ccagcgcaaa ccacagtttg ccctctttgc
    43621 tgttgctggt gaggaacacc gcacacaagg ccatgacttc ggccattacc acgaacatgc
    43681 cgaggttgct ggcaatgacg gcgcagacgg cggcagccat caacatattg atctgcaagc
    43741 cgttgcactt cacctgcgcg tggcgatgcc agtcaatgtt gtagaggctg acaaacagac
    43801 cgcacagacc gagcgtaatc agccaaatcg cgttaagcgg agagatttgc acatcgtagc
    43861 ttaccagcga cagcgcaccg ctcacgccaa ccgcgccagt cagtacagtg aagcccgcgg
    43921 ctgccgtata cagactacca accgcgccgc caattccggc tatccagccg cttagcgcct
    43981 tttgaaaaga aaagagaaat gccagaacag cggcggcgac aaaccacgcc acgccgctat
    44041 tgatcaggga aattgcgctc atttagcctc tccactttga gcctgctgaa acaaggtgag
    44101 atcgccaaag tccgtgttaa aggtcagctc acgcttacgt ttgctgacgc gggcgatatc
    44161 ggtgttatcc accagatgca gggctttagt cgggcacatc cgcacgcagg ccggaccttg
    44221 ttcatcaaag ctacaaaggt cacatttgac ggcgatcgcg cgaatacccg gcacccagtc
    44281 aagcagcgta ctgatacgcg ccggagcagg cggtgccggt ggcgctttcg gggtattggc
    44341 atttgcagga atatccagcg gacggctgcc ggaaaattca attgcgccaa acgggcaggc
    44401 gatgccgcac agcttgcagc ttacgcacag gctttcattc aactgcacgg ccccatcaac
    44461 gcgggtgatg gcgttaaccg ggcagaccac cgcgcagggt gcatcttcac agtggtgaca
    44521 gagctgcggc gcagattctt tttcatttag catcactctc aggcgcggca ttgattgcag
    44581 gccgtgctgg cgatgcgtct ctgaacaggc ggcctcacaa gtgtggcaac cgatacagag
    44641 cgtggagtca gcaattacaa aacgattcac caggcattcc tcaggtgatt gtcatttttg
    44701 acgaaaacat gccgatgaaa tgtcattttc gacactcatc gacacgccca tccccaaaca
    44761 ggcgtaacgc ctgcaaaacg ggcaaagcct cagctcatgc tgccgggctt tgtcccttta
    44821 ccagttggct taaattcacc ggcacattgt tttcaatagc ggtgtataag ctgtcgtaaa
    44881 cgccagcgat tttttcgaga tagtgttcca gaacctgttc gcgatcgcgg atgttgacct
    44941 tgccatcaat catgccgtca atgctcagtt gcgcctgcgc aatccgctgt ttgtcttcgc
    45001 catcttttgt ttcgacgtaa tactcgatgg tgtggctgtt aaagccatcc gccagggtga
    45061 cgtaaatacg aaaatgttca aaaaggacga aacagagttc tttgtccggc gcgcagctca
    45121 tggcgtgatg caggcgcgct ttcgataacg tgatcccctg aaccagcgaa ttgcagtaga
    45181 tgtgccactg gtcctgtagg cgacgatgcc gctgtgcgat gtaatcggct ttctcgctta
    45241 tttcccaaat agtcattgtc aggttacccg tttaacagag atgccagctt taagcatttt
    45301 ttgtgccaac ttttaattta ttattattaa agcgattttt aagattttgg tgaatttcgt
    45361 cgccgtgtcg acgtgtcatt tcgacatcat cgacattatt cactgcaggg ataatcaaca
    45421 ctggcacaat tattgcttgt agctggcaat agttaatggg aggcgatatg cacgaaataa
    45481 ccctctgcca acgggcactg gaattgatcg aacagcaggc cgcaaaacac ggcgcaaaac
    45541 gcgtaactgg ggtctggctc aaaattggcg cattttcttg tgtcgaaacc agctctcttg
    45601 ccttttgttt tgatctggtt tgccgcggca gcgtggcgga aggttgtaaa ctgcacctcg
    45661 aagaacaaga ggccgaatgc tggtgtgaaa catgccaaca gtatgtgacg ctactgaccc
    45721 agcgcgtccg ccgctgtcca cagtgtcatg gtgacatgct acagattgtg gcagacgacg
    45781 gtttacagat tcggcggata gaaatagacc aggagtgagc gatgtgtaca acatgcggtt
    45841 gcggtgaagg caacctgtat atcgagggtg atgaacataa ccctcattcc gcgtttcgta
    45901 gcgcgccatt tgccccggcg gcacgcccga agatgaaaat caccggcatt aaagcgcctg
    45961 aatttacccc cagccagact gaagaaggcg acctgcatta cggtcatggc gaagcgggca
    46021 ctcacgcacc gggcatgagc cagcgtcgga tgctggaagt cgaaattgac gtgctggaca
    46081 aaaataaccg tctggctgaa cgcaaccgcg cgcgctttgc tgcctgcaag caactggtgc
    46141 tcaacctggt ttccagccca ggttccggta aaaccaccct gctgacggaa accttaatgc
    46201 gcctgaaaga cagcgttccg tgcgcggtta ttgaaggtga ccagcaaacc gtgaacgatg
    46261 ccgcacgcat tcgcgctacc ggcacaccag cgattcaggt gaacaccggt aaaggctgcc
    46321 atcttgacgc acagatgatt gccgacgccg caccgcgtct gccactggac gataacggta
    46381 ttctgtttat cgaaaacgtt ggcaacctcg tatgcccggc cagcttcgat ctcggtgaaa
    46441 aacacaaagt ggcggtgctt tccgttaccg aaggtgaaga caaaccgctg aaatatccgc
    46501 atatgtttgc cgccgcctcg ctgatgctgc tcaacaaagt tgacctgttg ccgtatctca
    46561 actttgacgt tgagaagtgc atcgcctgcg cccgcgaagt caatccagaa attgaaatca
    46621 tccttatttc cgccaccagc ggcgaaggga tggaccagtg gctgaactgg ctggagacac
    46681 agcgatgtgc ataggcgttc ccggccagat ccgcaccatt gacggtaacc aggcgaaagt
    46741 cgacgtctgc ggcattcagc gcgatgtcga tttaacgtta gtcggcagct gcgatgaaaa
    46801 cggtcagccg cgcgtgggcc agtgggtact ggtacacgtt ggctttgcca tgagcgtaat
    46861 taatgaagcc gaagcacgcg acactctcga cgccttgcaa aacatgtttg acgttgagcc
    46921 ggatgtcggc gcgctgttgt atggcgagga aaaataatgc gttttgttga tgaatatcgc
    46981 gcgccggaac aggtgatgca gttaattgag catctgcgcg aacgtgcttc acatctctct
    47041 tacaccgccg aacgccctct gcggattatg gaagtgtgcg gtggtcatac ccacgccatt
    47101 tttaaattcg gcctcgacca gttactgcct gaaaacgttg agtttatcca cggtccgggt
    47161 tgcccggtgt gcgtactgcc gatgggcaga atcgacacct gcgtggagat tgccagccat
    47221 ccggaagtca tcttctgtac ctttggcgac gccatgcgcg tgccggggaa acagggatcg
    47281 ctgttgcagg caaaagcacg cggtgccgat gtgcgcatcg tctattcgcc gatggatgcg
    47341 ttgaaactgg cgcaggagaa tccaacccgc aaagtggtgt tcttcggctt aggttttgaa
    47401 accaccatgc cgaccaccgc catcactctg caacaggcga aagcgcgtga tgtgcagaat
    47461 ttttacttct tctgccagca tattacgctt atcccgacgc tgcgcagttt gctggaacag
    47521 ccggataacg gtatcgacgc gttcctcgcg ccgggccacg ttagtatggt tatcggcact
    47581 gatgcctata attttatcgc cagcgatttt cagcgtccgc tggtggtggc tggtttcgaa
    47641 ccccttgatc tactgcaagg cgtggtcatg ctggtggagc agaaaatagc ggcccacagc
    47701 aaggtagaga atcagtatcg tcgggtggta ccggatgccg gtaacctgct ggcgcaacag
    47761 gcgattgccg atgtgttctg tgtcaacggc gacagcgaat ggcgcggctt aggcgtgatt
    47821 gaatcttctg gcgtgcacct gacgccggat tatcaacgat tcgatgccga agcacatttc
    47881 cgcccggcac cgcagcaggt ctgcgatgac ccgcgcgcgc gttgtggcga agtcttgacg
    47941 ggcaaatgta agccgcatca atgcccgctg tttggtaaca cctgtaatcc tcaaaccgcg
    48001 tttggtgcgc tgatggtttc ctccgaagga gcgtgcgccg cgtggtatca gtatcgtcag
    48061 caggagagtg aagcgtgaat aatatccaac tcgcccacgg tagcggcggc caggcgatgc
    48121 agcaattaat caacagcctg tttatggaag cctttgccaa cccgtggctg gcagagcagg
    48181 aagatcaggc acgtcttgat ctggcgcagc tggtagcgga aggcgaccgt ctggcgttct
    48241 ccaccgacag ttacgttatt gacccgctgt tcttccctgg cggtaatatc ggcaagctgg
    48301 cgatttgcgg caccgcgaat gacgttgcgg tcagtggcgc tattccgcgc tatctctcct
    48361 gtggctttat cctcgaagaa ggattgccga tggagacact gaaagccgta gtgaccagca
    48421 tggcagaaac cgcccgcacg gcaggcattg ccatcgttac tggcgatact aaagtggtgc
    48481 agcgcggcgc ggcagataaa ctgtttatca acaccgcggg catgggcgca attccgacga
    48541 atattcactg gggcgcacag acgctaaccg caggcgatat attgctggtt agcggtacac
    48601 tcggcgacca cggggcgact atccttaacc tgcgtgagca gctggggctg gatggcgaac
    48661 tggtcagcga ctgcgcggtg ctgacgccgc ttattcagac gctgcgtgac attcccggcg
    48721 tgaaagcgct gcgtgatgcc acccgtggtg gtgtaaacgc ggtggttcat gagttcgcgg
    48781 cagcctgcgg ttgcggtatt gaaatttctg aatcagcgct gccggttaaa cctgccgtgc
    48841 gcggcgtttg cgaattgctg ggactggacg ccctgaactt tgccaacgaa ggcaaactgg
    48901 tgatcgccgt tgaacgcaac gcggcagagc aagtgctggc agcgttacat tcccatccac
    48961 tggggaaaga cgcggcgctg attggtgaag tggtggaacg taaaggtgtt cgtcttgccg
    49021 gtctgtatgg cgtgaaacga accctcgatt taccacacgc cgaaccgctt ccgcgtatat
    49081 gctaataaaa ttctaaatct cctatagtta gtcaatgacc ttttgcaccg ctttgcggtg
    49141 ctttcctgga agaacaaaat gtcatataca ccgatgagtg atctcggaca gcaagggttg
    49201 ttcgacatca ctcggacact attgcagcag cccgatctgg cctcgctgtg tgaggctctt
    49261 tcgcaactgg taaagcgttc tgcgctcgcc gacaacgcgg ctattgtgtt gtggcaagcg
    49321 cagactcaac gtgcgtctta ttacgcatcg cgtgaaaaag acacccccat taaatatgaa
    49381 gacgaaactg ttctggcaca cggtccggta cgcagcattt tgtcgcgccc tgatacgttg
    49441 cattgcagtt acgaagaatt ttgtgaaacc tggccgcagc tggccgcagg tgggctatac
    49501 ccaaaatttg gtcactattg cctgatgcca ctggcggcgg aagggcatat ttttggtggc
    49561 tgtgaattta ttcgttatga cgatcgcccc tggagcgaaa aagagttcaa tcgtctgcaa
    49621 acatttacgc agatcgtttc tgtcgtcacc gaacaaatcc agagtcgcgt cgttaacaat
    49681 gtcgactatg agttgttatg ccgggaacgc gataacttcc gcatcctggt cgccatcacc
    49741 aacgcggtgc tttcccgcct ggatatggac gaactggtca gcgaagtcgc caaagaaatc
    49801 cattactatt tcgatattga cgatatcagt atcgtcttac gcagccaccg taaaaacaaa
    49861 ctcaacatct actccactca ctatcttgat aaacagcatc ccgcccacga acagagcgaa
    49921 gtcgatgaag ccggaaccct caccgaacgc gtgttcaaaa gtaaagagat gctgctgatt
    49981 aatctccacg agcgggatga tttagccccc tatgaacgca tgttgttcga tacctggggc
    50041 aaccagattc aaaccttgtg cctgttaccg ctgatgtctg gcgacaccat gctgggcgtg
    50101 ctgaaactgg cgcaatgtga agagaaagtg tttaccacta ccaatctgaa tttactgcgc
    50161 cagattgccg aacgtgtggc aatcgctgtc gataacgccc tcgcctatca ggaaatccat
    50221 cgtctgaaag aacggctggt tgatgaaaac ctcgccctga ccgagcagct caacaatgtt
    50281 gatagtgaat ttggcgagat tattggccgc agcgaagcca tgtacagcgt gcttaaacaa
    50341 gttgaaatgg tggcgcaaag tgacagtacc gtgctgatcc tcggtgaaac tggcacgggt
    50401 aaagagctga ttgcccgtgc tatccataat ctcagtgggc gtaataatcg ccgcatggtc
    50461 aaaatgaact gcgcggcgat gcctgccgga ttgctggaga gcgatctgtt tggtcatgag
    50521 cgtggggctt ttaccggtgc cagcgcccag cgtatcggtc gttttgaact ggcggataaa
    50581 agctccctgt tcctcgacga agtgggcgat atgccactgg agttacagcc gaagttgctg
    50641 cgtgtattgc aggaacagga gtttgaacgc ctcggcagca acaaaatcat tcagacggac
    50701 gtgcgtctaa tcgccgcgac taaccgcgat ctgaaaaaaa tggtcgccga ccgtgagttc
    50761 cgtagcgatc tctattaccg cctgaacgta ttcccgattc acctgccgcc actacgcgag
    50821 cgtccggaag atattccgct gctggcgaaa gcctttacct tcaaaattgc ccgtcgtctg
    50881 gggcgcaata tcgacagcat tcctgccgag acgttgcgca ccttgagcaa tatggagtgg
    50941 ccgggtaacg tacgcgaact ggaaaacgtc attgagcgcg cggtattgct aacacgcggc
    51001 aacgtgctgc agctgtcatt gccagatatt gctttaccgg agcctgaaac gccgcctgcc
    51061 gcaacggttg tcgctcagga gggcgaagat gaatatcagt tgattgttcg cgtgctgaaa
    51121 gaaactaacg gcgtggttgc cgggcctaaa ggcgctgcgc aacgtctggg gctgaaacgc
    51181 acgaccctgc tgtcacggat gaagcgactg ggaattgata aatcggcatt gatttaactg
    51241 caaattgccg gacagatctg cctgtccggc atactattca cgaggttttt tcggacgata
    51301 tttttccggc agttctggca ccggacactt gtcatcgatg agatgacgca cggttaagat
    51361 cggatgacgc cacagcattc tcggcccggc ccaacgcata atctgtttca tctcttcacg
    51421 ctttgcaggc tggtaacagt gcaccggaca ctgcttacag gctggtttct cttcgccgaa
    51481 cacacattta tccagccgct tttgcgcgta aacaaacaac gcctcgtaat gctccggctc
    51541 cgctgacgcc tgcgggcatt tcgcttgata aagatcgatc atttttttaa tcgtcagttt
    51601 ttcacgagag atacgcttgc cggacatact gcctccacct cattaagatg catttatatt
    51661 acaacttaat cttaaagggc actatgactc taaagaagaa gggttagcca accgatacaa
    51721 ttttgcgtac ttgcttcata agcatcacgc aaaagctgca aaacagcatc tttcccggaa
    51781 ccagcatcaa gaactcgccg ttcgcttctt cccctgaaat gattaactcc ggtatcatgt
    51841 gcgccttatg tgattacaac gaaaataaaa accatcacac cccatttaat atcagggaac
    51901 cggacataac cccatgagtg caatagaaaa tttcgacgcc catacgccca tgatgcagca
    51961 gtatctcaag ctgaaagccc agcatcccga gatcctgctg ttttaccgga tgggtgattt
    52021 ttatgaactg ttttatgacg acgcaaaacg cgcgtcgcaa ctgctggata tttcactgac
    52081 caaacgcggt gcttcggcgg gagagccgat cccgatggcg gggattccct accatgcggt
    52141 ggaaaactac ctcgccaaac tggtgaatca gggcgagtcc gttgccatct gcgaacaaat
    52201 tggcgatccg gcgaccagca aaggtccggt tgagcgcaaa gttgtgcgta tcgttacgcc
    52261 aggcaccatc agcgatgaag ccctgttgca ggagcgtcag gacaacctgc tggcggctat
    52321 ctggcaggac agcaaaggtt tcggctacgc gacgctggat atcagttccg gtcgttttcg
    52381 cctgagcgaa ccggctgacc gggaaacgat ggcggcagaa ctgcaacgca ctaatcctgc
    52441 ggaactgctg tatgcagaag attttgctga aatgtcgtta attgaaggcc gtcgcggcct
    52501 gcgccgtcgc ccgctgtggg agtttgaaat cgacaccgcg cgccagcagt tgaatctgca
    52561 atttgggacc cgcgatctgg tcggttttgg cgtcgagaac gcgccgcgcg gactttgtgc
    52621 tgccggttgt ctgttgcagt atgcgaaaga tacccaacgt acgactctgc cgcatattcg
    52681 ttccatcacc atggaacgtg agcaggacag catcattatg gatgccgcga cgcgtcgtaa
    52741 tctggaaatc acccagaacc tggcgggtgg tgcggaaaat acgctggctt ctgtgctcga
    52801 ctgcaccgtc acgccgatgg gcagccgtat gctgaaacgc tggctgcata tgccagtgcg
    52861 cgatacccgc gtgttgcttg agcgccagca aactattggc gcattgcagg atttcaccgc
    52921 cgagttgcag ccggtactac gtcaggtcgg cgacctggaa cgtattctgg cgcgtctggc
    52981 gttgcgtacc gctcgcccac gcgatctggc ccgtatgcgt cacgctttcc agcaactgcc
    53041 ggagctgcgt gcgcagttag aaactgttga tagtgcacca gtacaggcgc tacgtgagaa
    53101 gatgggcgag tttgccgagc tgcgcgatct gctggagcga gcaatcatcg acacaccgcc
    53161 ggtgctggta cgcgacggtg gtgttatcgc atcaggctat aacgaagagc tggatgagtg
    53221 gcgcgcgctg gctgacggcg cgaccgatta tctggagcgt ctggaagtcc gcgagcgtga
    53281 acgtaccggc ctggacacgc taaaagttgg ctttaatgcg gtgcacggct actacattca
    53341 aatcagccgt gggcaaagcc atctggcacc tatcaactat atgcgtcgcc agacgctgaa
    53401 aaacgccgag cgctacatca ttccagagct aaaagagtac gaagataaag tcctcacttc
    53461 aaaaggcaaa gcactggctc tggaaaaaca gctttatgaa gagctgttcg acctgctgtt
    53521 gccgcatctg gaagcgttgc aacagagcgc gagcgcgctg gcggaactcg acgtgctggt
    53581 gaacctggcg gaacgggcct ataccctgaa ctacacctgc ccgaccttca ttgataaacc
    53641 gggcattcgc attaccgaag gccgccatcc ggtggttgaa caggtgctga acgagccatt
    53701 tatcgccaac ccgctgaatc tgtcaccgca gcgccggatg ttgattatta ccggtccgaa
    53761 catgggcggt aaaagtacct atatgcgcca gaccgcactg attgcgctga tggcctatat
    53821 cggcagctac gtaccggcgc aaaaagtcga gattggcccg attgaccgta tctttacccg
    53881 cgtaggggca gcggatgatc tggcttccgg gcgttcaacc tttatggtgg agatgaccga
    53941 aaccgctaat attctgcata acgccaccga gtacagtctg gtgctgatgg atgagattgg
    54001 gcgcggaacg tccacttacg atggtctgtc gctggcgtgg gcgtgcgcgg aaaatctggc
    54061 gaataagatt aaggcgttga cgctgtttgc cacccactat ttcgagctga cccagttacc
    54121 ggagaaaatg gaaggcgtcg ccaacgtgca tctcgatgca ctggagcacg gcgacaccat
    54181 tgcctttatg catagcgtgc aggatggcgc ggcgagcaaa agctacggcc tggcggttgc
    54241 agctctggcc ggcgtgccaa aagaggttat taagcgcgca cggcaaaaac tgcgtgagct
    54301 ggaaagcatt tcgccgaacg ccgccgctac gcaagtggat ggtacgcaaa tgtctttgct
    54361 gtccgtaccg gaagaaacct cgcctgcagt cgaggcactg gaaaacctcg atccggattc
    54421 actcaccccg cgtcaggcgc tggaatggat ttatcgcctg aagagtctgg tgtaataata
    54481 attcccgata gtcttttgct atcgggaata ttaacgataa ctgacgaatc aaataaaaat
    54541 accctgtata ataggaaagc ttattttaca gggtaaaacc atgccatcta cacgctatca
    54601 aaaaatcaat acccatcact atcgccatat atgggtcgtt ggtgatattc atggtgaata
    54661 tcagttatta caatcccgct tacatcaact ctcttttttc cccgaaaccg acttacttat
    54721 ttctgtcggc gataatattg atcgtgggcc ggagagtctt aacgtcctgc gcctgctaaa
    54781 ccagccctgg tttatctccg ttaaaggcaa ccacgaagca atggcgctgg atgcgttcgc
    54841 gaccggcgat ggcaatatgt ggcttgccag cggtggtgac tggtttttcg atttaaatga
    54901 ttcagagcaa aaagaagcta cagatctgtt gctgaaattc catcaccttc cacatattat
    54961 tgaaatcact aacgacaaca taaaatatgt catcgcacat gcagattatc cggggagtga
    55021 atatctcttt ggtaaagaaa tagcggagag cgaattactc tggcctgttg atcgtgtgca
    55081 gaaatcgctt aatggcgagt tacaacaaat aaacggcgct gattatttta tatttggaca
    55141 tatgatgttt gataacattc agacgttcgc taaccagatt tatattgata ccggatcgcc
    55201 gaaaagcggg cggctgtcat tttataaaat aaagtaatga ggccgggtaa agcaaagcca
    55261 ctacccggca ctttttatta gcgcttacct tccgccagca gcggcgggat gctcggcacc
    55321 attggcgcgt catcaatatc tttctgcgtc atgcggaacg cttcgggata atgttcgcgg
    55381 ctggtacgac gcagcggttc ggtatcgcgc caggtataaa ggcaatgctg gcactgatat
    55441 accgtccaga catctttcac cggcgatttc gccatcactt caatctgttc atcggcacaa
    55501 cgtggacaaa tcatcttgtt ctccttattt acgtgcggcc agcatagcgg tcagtttttc
    55561 agcccaggct ttggtttccg gcaaatccac caccggctgg ctgtagtgac cacggttgtc
    55621 cggggcgaca ggcgtggtgg cgtcgataat cagcttgtcg gtgatccccg ccgggcttga
    55681 gccagggtcg agttccagca cggacatatt cggcaactgc accaaatccc ctgccggatt
    55741 tactttcgag gagagcgccc acatcacctg cggcaggttg aacgggtcaa cgtcttcatc
    55801 gaccataatc accatcttta cgtagcccag accgtgcggc gtggtcatcg cacgcaggcc
    55861 caccgcgcgg gcaaagccgc cgtagcgttt tttggtggag ataatcgcca gcaggccgtg
    55921 ggtgtacatg gcgtttaccg cctgcacttc cgggaactcg gctttcagtt gttgatacag
    55981 cggcacacag gtggctggcc ccatcaggta gtcgatttcg gtccacggca tgccgaggta
    56041 cagcgattcg aaaatcggcc tggtgcggta agagacttta tcgatacgca ccacggtcat
    56101 gttacgcccg ccggagtagt gcccggtaaa ctcaccgaac ggcccttcga tttcgcgttt
    56161 gcggctttcg ataacccctt cgaggatcac ttctgaaccc cacggcacat caaaaccagt
    56221 caatggcgcg gtggcgatcg ggtacgggct ttcgcgcagc gcgcctgcca tttcatactc
    56281 agactgatcg tatttcagcg gcgtggcgcc cataagggtg atgatcggat cgttacccag
    56341 ggtgatggca atcggcagat cttcaccgcg ctcttccgct ttatgcagat gcagggcgat
    56401 atcgtgcatc ggcaccggtt gcaggccgag cttacgcttg cccttcactt ccatgcggta
    56461 gataccgacg ttctgcttgc cgaagttatc cgggtcgagc ggatcgcggg aaaccacgca
    56521 cgctttgtcg agatagaaac cgccatcacc gtcgtttaaa cgaaacagcg gcaggatatc
    56581 gaacagatta atctcgtcac catcgacggt gttctgcgcc caggctggat tggcgcggcg
    56641 ctccggggcg atcgggaagt tatcccagcg gcggataaac tcatcaatct gttttttaac
    56701 cggggtgttt ggcggcaggc ccaaggaaat cgcgtggttc tgccaggaac cgatggtatt
    56761 catcgccacg cgggcatcgg taaaaccgcg aatattatca aaccacagcg ccggtgcacc
    56821 atcgccgata cgcccggtgg cgttggcagc ggcagccaga tccggttccg cgttcacctc
    56881 ttcgctgatt ttcagtaact ggccgtggtc atcaagcgcc tgtaaaaagc tgcgtaaatc
    56941 atcaaatgcc attattcatt ctcctgagaa aaattccggg cctgcggcaa tccttgccag
    57001 cgcctggcgt agggatgttc aaggccaaat tgatccagca cgcgggctac cacgtggtgg
    57061 acaatgtcat ctaccgtttc gggatggtta taaaaggcag gcatcggcgg caccatcgcc
    57121 acgcccatgc gtgaaagtgc gagcatattt tcgagatgga tggtgctaag cggcatttca
    57181 cgcggcacca gcaccagttt gcggccttct ttaagcacga cgtccgccgc gcgccctacc
    57241 aggccatcag cgtaaccagc gcggataccg gcgagcgttt tcatactgca cggaataacg
    57301 atcatgccgt ctgtacgaaa ggaacctgag gagatggtcg ccgcctgatc cgccgggtta
    57361 tggctgaagt cagcgagggc agcaacatcg cgggcgctgt aaggcgtttc cagttcaatg
    57421 gtggttttcg cccacttcga catcaccaga tgagtctcga cattcggcat ctcccgcagc
    57481 gcttgcagta atgccacacc aagaggcgca ccggtagccc ctgtcatccc gacgatcagt
    57541 ttcattgctt actctcttaa ttgttcgtat acgaacatta ttattaaaat acgcctctgt
    57601 tctcattgcg gcaagcactg aaaagtaaca acatcccctt cgtcactgaa aaaccgctat
    57661 gatagcggca gatagtttgc ccggagttta catgacgtta cgaaataaag cgttccatca
    57721 gttacgacag ctttttcagc agcacacggc tcgctggcag cacgagttac ctgacctgac
    57781 caaaccacag tatgcggtga tgcgcgccat tgctgataag cctggtatcg aacaggtggc
    57841 actgatagaa gcagcagtca gcaccaaagc aacgttggca gaaatgttgg caagaatgga
    57901 gaatcgtggc ttagtcagac gtgaacatga tgccgctgac aagcggcgtc gctttgtctg
    57961 gctgactgcc gaaggagaaa agatacttgc tgcggcgata ccgattggtg acagcgtgga
    58021 tgaggaattt ttggggcgtt tgagtgcaga agagcaggag ctgtttgtgc agctggtgcg
    58081 caagatgatg aacacatagg atgcaaattg ccgggtagga cgctgacgtg tcttatccag
    58141 gcgacaaaaa cacaagcttc ccaaacaaaa gaaaaaggcc agcctcgctt gagactggcc
    58201 tttctgacag atgcttactt actcgcggaa cagcgcttcg atattcagcc cctgcgtttg
    58261 caggatttcg cgcaaacggc gcaggccttc aacctgaatc tggcgaacac gttcacgggt
    58321 gaggccaatt tcacgaccta catcttccag tgttgccgct tcgtacccca gcaaaccgaa
    58381 tcgacgtgcc agtacttcac gctgtttggc gttcagctcg aacagccatt tgacgatgct
    58441 ctgcttcata tcgtcatctt gcgtggtatc ttccggaccg ttctcttttt catcggccag
    58501 gatgtccagc aacgcttttt cggaatcacc acccagcggg gtgtctaccg aggtaatgcg
    58561 ctcgttaaga cgaagcatac ggctgacgtc atcaactggc ttatccagtt gctctgcgat
    58621 ctcttccgca cttggttcat ggtcagctta tgggacaact cacgtgcggt tcgcaggtaa
    58681 acgttcagct cctttacgat gtgaatcggc aaacgaatag tacgggtttg gttcataatc
    58741 gcccgttcaa tcgtctggcg aatccaccag gttgcgtatg ttgagaagcg gaaaccacgt
    58801 tccgggtcaa acttctctac cgcacggatc agccccaggt tgccctcttc gataaggtcc
    58861 agcaacgcca gaccacgatt gccataacgg cgggcaattt ttaccaccag acgcaagtta
    58921 ctctcgatca tccggcggcg agaggcgaca tctccacgca gtgcgcgacg cgcaaaataa
    58981 acttcttctt cggccgttaa cagtggtgaa taaccaatct caccaaggta aagctgagtc
    59041 gcgtccaaca cacgctgtgt ggctccctgc gataacagtt cctcttcggc caaatcgtta
    59101 tcactgggtt cctcttctac taaggccttt tcgtcaaaaa cctcaactcc gttctcatca
    59161 aattccgcat cttcatttaa atcatgaact ttcagcgtat tctgactcat aaggtggctc
    59221 ctacccgtga tcccttgacg gaacattcaa gcaaaagcct ggttccgccg atttatcgct
    59281 gcggcaaata acgcagcggg tttacggatt tccccttgta acgaatttca aaatgcaagc
    59341 gtgttgaact ggttccggtg ctacccatgg ttgctatttt ttgccccgcc ttcacttctt
    59401 gttgttcccg gaccagcatt gtgtcgttat gggcgtaggc actcaggtaa tcatcattat
    59461 gtttgatgat aatcagatta ccgtagccgc gcagcgcgtt accggcataa acaacacggc
    59521 catctgcggt cgcgataatt gcctgtcctt tgctgcctgc gatatcaatc cccttgttgc
    59581 ccccctcaga agcgccaaag gtttcgatca ctttgccctc agtcggccag cgccaggtgg
    59641 agataggcgt actggttgat gtactgctga cagtcggctc ggttgtgctt gctgttggta
    59701 ccgttacagg cgctgtgacc gtggtcgcag ttggcttgtt gttcggcaac attttgttag
    59761 cactctgttc acccgaagac tcagaatacg taattgtcgg ttgcgacgca acagcaacgg
    59821 tggaattttg tgcaggcttg atcacaactc cttgctctgc tgcgtcggcc tgggtaatgg
    59881 catttccgcc agtgattggc gtaccggaag cattacccac ctgtaaggtc tgaccgacgt
    59941 tcagcgcgta tggtgcctga atattgttgc gctgagcaag gtcacggaaa tcgttgccag
    60001 taatccaggc gatatagaaa agcgtgtcgc cttttttcac ggtataggta ctgccgctat
    60061 aactgccttt cggaatgttc ccatactgac ggttatagac gatgcgtccg ttttccatct
    60121 gtaccggctg ctgagctact ggctgcactg gctggatttg cggttgctgc gctgtagacg
    60181 tcgtccccat tttcggcggc ggcgtaatca acataccaga attggtatgt gcaggcgcat
    60241 tgccattaac ggagctgacc ggtgccggtg gatttgaagt gtcagaacag cctgccagcc
    60301 atagcgaaac cagtgacaaa gccgcaatgc ggcgaacggt gaattttggg cttcccgcgc
    60361 tcatttatcc cccaggaaaa attggttaat aaccagtgac ataattaccg tgcaaggcac
    60421 catactgaac actggaaaag atgttcacga tacgctgacc tgcggcaaaa taaccaggaa
    60481 aaatccaggt atttcctcac gttttaagcc agctcaccct tcactaaagg gacaaagcgc
    60541 acggcctcca cggtatcgat aataaattcg cctccccgac gacgcacccg tttcaaatac
    60601 tggtgctcct cccctacggg taagacgaga atcccgcctt cgtccagctg cgtcattagc
    60661 gcagttggaa tttccggcgg tgccgccgta acaatgatag cgtcaaacgg cgcacgtgcc
    60721 tgccaacctt gccatccatc gccatgacgg gttgaaacat tatgtaaatc aagatttttc
    60781 aggcggcgac gcgcctgcca ctgcaagcct ttaatccgtt caaccgagca aacatgctgg
    60841 acaagatgcg ccaggattgc cgtttgatat cccgaaccgg tgccaatttc cagcacccgc
    60901 gactgcggcg tcagctcgag taattcggtc attcgcgcca ccatatacgg ctgcgaaatc
    60961 gtctgcccct gacctatcgg caaggcgata ttgtcccagg ctttttgttc aaacgcttca
    61021 tcaacgaatt tttcacgcgg cacggcggca agtgcattca gcacctgctc atcctgaata
    61081 ccttgcgcac gtaattgatc cagaagtgct tgtacgcgtc tgcttaccat tgcgtgccaa
    61141 ctcccacgct gtttaaccag tctgaaacca catcttgcgc gctatgcgca gttaaatcca
    61201 catgcagcgg cgtgatggag acatagccct catctaccgc agcaaaatcg gtccccggac
    61261 cggcatcaca tttaccgccc ggcgggccaa tccagtacag cgtattgccg cgcggatctt
    61321 gctgcgggat cacctgatct gccggatgtc gtgtaccgca gcgcgtcacg cgaatacctt
    61381 tgatttgatc caagggtaaa tccggaacat taatattaag aatacgcccg gtgcgcagcg
    61441 gctctttaca cagtgcgcgc aaaattgaac aggttaccgc cgcggcagtg tcgtaatgtt
    61501 tatgcccgtc aagcgagacg gcaagcgccg gaaaacctaa atgacggcct tccatcgcgg
    61561 cggctaccgt accggaataa ataacatcat cccccagatt cggcccggcg ttaattccgg
    61621 acacaacaat gtccgggcgc ggacgcatca gagcattcac gccaagatag acgcaatcgg
    61681 tcggggttcc catttgcaca gcaatatcac cattttcaaa ggtaaacgtg cgcagggagg
    61741 attccagtgt cagagaattt gaagcgccgc tgcggttacg atcgggggcg accacctgaa
    61801 cgtcagcaaa ctcacgcaag gctttcgcca gcgtttgtat accgggtgca tgtaccccgt
    61861 catcattact cagcaatatg cgcataatca cctgttgtgt tgataagttc cctgacaacg
    61921 ctggttgcaa aactacccgc cggaagccag aaacggattt ctacggtgac gtcatcccac
    61981 caattccagc ttaattgttg cggatacagc agcatcgctc tgcgcgcggc ttcaactttt
    62041 tcgcgcacca gtaaagcttg taattcagtt tctgcggcga cagctgcctg ttcgaatgcc
    62101 agcgcttcac gctgagttcc ccattcacca ctgcccggca atgcggcggt tatcatcagc
    62161 tctttatcgt tgacgcgacg ctgtaattcc accagttctt cggtggttgc aacaaaccag
    62221 ctaccacgtc cggctaattg tagcgcatcg ccgtcaacaa cttgattaac gtctgctttt
    62281 ttgaggcgct cagcaacaat ctgattaaac aacgcactgc gggctgccga caaccaaaaa
    62341 ctccgtttat tgcgatcgcg caccggagta ttggtttgcg cccagcgcag cgcgccctgc
    62401 aagttgctac cgccaatccc aaaacgttgg gcaccgaagt agttcggtac accttttacg
    62461 caaatatcga tcagacgttg ttcaacgtca tcgcgattgc tcacttcgcg cagaaccagg
    62521 gtaaaggcgt tacctttcag cgcccctaaa cgcagcttgc gcttgtgccg cgcatactcc
    62581 agcacctggc agccttccaa ttgaaaggcg ctcagatcgg gcatttcctt gcccggcacg
    62641 cgagcgcata accactgttc tgtaacagca tgtttgtctt tttgcccagc aaagctgact
    62701 tcacgggcat gaattttcag gaatttcgcc agtgcatccg ccacaaaacg ggtattgcag
    62761 ccgtttttga ggattctaac cagaatatgc tcaccttcac catcaggctc aaagcccaaa
    62821 tcttccacca ccacaaagtc ttccggattg gctttcagca gcccggtgcc ttgcggttta
    62881 ccgtggaggt aagtgagatt atcaaactca atcattttgt tgccttaatg agtagcgcca
    62941 ccgcttcaca ggcaatccct tccccacgtc cggtaaatcc aagtttttcc gtagtagtgg
    63001 ctttcacgtt aacatcatcc atatggcagc cgagatcttc ggcaataaac acgcgcattt
    63061 gtggaatgtg cggcaacatc ttcggtgcct gagcgatgat agtgacatcg acgttgccaa
    63121 gggtataacc cttcgcctga atacgacgcc aggcttcgcg tagcagctcg cggctatcgg
    63181 cacctttaaa tgccggatcg gtatccggga acagtttgcc gatatccccc agcgccgccg
    63241 cgccaagcaa tgcatcggtc aacgcatgga gcgccacgtc gccatcagaa tgcgccagca
    63301 atcctttttc gtaaggaatg cgtacgccac caatgataat tgggccttca ccgccaaagg
    63361 cgtgtacgtc aaaaccgtgt ccaattcgca ttatgtattc tcctgatgga tggttcgggt
    63421 gaggtaaaac tcggccagtg ccaaatcctc cgggcgcgtg actttaatgt tatccgcacg
    63481 gccttcgacc aactgaggat ggaatccgca atattccagc gccgaggctt cgtcggtaat
    63541 agccgcgcct tcatttagag cgcgcgtcag acagtcatgt aacagctcac gagggaaaaa
    63601 ttgcggcgtc agcgcgtgcc ataagccgtt gcgatcaacg gtatgagcaa tggcattttt
    63661 gcccggttcg gcacgtttca tagtatcgcg cactggtgcg gctaggatcc ctcccgtgcg
    63721 gctggtttcg ctcaacgcca acaatcgcgc gaggtcatcc tgatgcagac aaggacgagc
    63781 ggcgtcatgc accaataccc actgcgcgtc gccagcggct ttcaaacctg ccagcacgga
    63841 atcggcacgc tcatcaccgc catctacaac ggtgatttgc ggatgattcg ccagaggaag
    63901 ttgtgcaaaa cggctatcgc caggacttat ggcaatgacg acacgtttca cccggggatg
    63961 cgccagcagc gcatacaccg agtgttcaag aatggtttga ttaccgattg agagatattg
    64021 cttaggacat tccgtttgca ttcgacggcc aaatccggcc gccggaacca cggcgcaaac
    64081 atccaaatga gtggttgcca tgttaattcc cgggctgatt tatcgattgt tttgccccgc
    64141 agactgtgcg cgcttcgacg cgtcaggcac cagacgataa aaagtttcgc ccggcctggt
    64201 catgctgagt tcattacgcg cacgctcttc gagcgcctcc tggccgccat tgagatcgtc
    64261 aatttcggca aaaagttgat cgtttcgcgc tttaagtttc gcgtttgtag cttgctgtgc
    64321 cgccacatca tcattgacgc gggtatagtc atgtataccg ttcttaccga accacagcga
    64381 atactgtagc cagaccagaa tagccagcaa cagcagcgtt agtttaccca tcctgccccc
    64441 tgaaaaacgg catcatcatc ccatgcatcc gaagacgact ctacatcctc tgttggggat
    64501 accgcgacaa cgcgggcaaa tgtaccacat ttgtccattg ttacgtatac ccagggcgtg
    64561 cagaacataa tctcattatt agttacggtt tgaattatga acagaggaga caagaaagta
    64621 caaattagcc cagtagccac ataaacagtg cgccaaacat aatgcctact gtcatcaggg
    64681 tgaaaacaat actgtagcgt agctttccgt ccatcaatga atgcagcgca atccccacca
    64741 ctaccgcaac gggcatcagc gccagaaaga aaggccaggt gtagataaag aagaacagcg
    64801 tgttagagcc ataaatcaac atcggcatcg ccagcgcaaa taaccaggag ataaaaccga
    64861 ccacggcacc aggcagtgac catgtggttt cttcatcctc agtaaggctg tcgttatttg
    64921 ttagtgtaat gttatggcta ttacgcatat ttgatcctgt tactttgacg aaccgggcat
    64981 ggaaacccgg tggtgtctca ggatctgata atatcgttct gtctcaacag atctaataat
    65041 tgctgtacca aatttgttac taattgttca ccattgagat gaatttctgc cgattcaggc
    65101 gcttcgtaaa cggaatctat tcccgtaaag ttgcgcagtt caccggcacg cgctttctta
    65161 tataagcctt ttggatcgcg ggcttcgcaa atcgccagtg gcgtatcgac aaacacttcg
    65221 ataaagcgcc cttctcctac gcgttcgcga accatctggc gttcggcgcg gtgtggcgag
    65281 ataaatgcgg tcagcaccac cagtccggct tcaaccatca aattcgccac ttcaccgacg
    65341 cgacggatat tctctttacg atcggcatcg ctaaaaccga gatcgctgca taatccgtgg
    65401 cgaacattgt cgccatccag cagatacgta ctgacgccga gtttatgtaa cgcctcctcc
    65461 agcgccccgg cgaccgttga tttaccggac ccggagaggc cggtaaacca cagcactaca
    65521 ccacgatgac cgtggtgtag ctcgcgttgt tgcacagtga ccggatggct atgccagacg
    65581 acgttttcgt catgcagcgc cattatttct cccccagcaa atcgcgcgcg ccccagtgtg
    65641 gaaagtggcg gcgaaccagg gcattcaatt ccagttcgaa tgcactaaat tcagatggcg
    65701 cagcagttgc ctggctaact ggctcatgca ccataccggc acctacggtc acattgctca
    65761 ggcgatcgat aaaaatcagc ccaccggtaa ccgggttttg ctgataacga tctaacacca
    65821 gtggctcgtc aaaagtgaga tccaccaggc cgatgccgtt cagcggcagg ttttcaactt
    65881 cgcgttgggt aaggttatta atatcaacct gataacgaat gccatcaaca cgagaacgcg
    65941 tcttcttacc ggcaattttg atgtcgtaac tctggcccgg ggaaagcggc tgttccgcca
    66001 tccataccac atccaccgac gcgctctgca cagctggtaa cgcttcgtct gccgccagca
    66061 gcagatcgcc acggctgatg tcgatctcat ccgtcagcac cagggtgata gcttctccgg
    66121 caaaggcttc ttcgcgatca ccatcaaaag tcacgatccg cgcgacgttt gattccacac
    66181 cagagggcag cacttttaca cgttgcccga cttccacgcg accggatgcc agcgttccgg
    66241 cgtagccacg aaaatcgaga tttgggcggt taacgtactg caccgggaag cgcattggct
    66301 gggcatccac cactcgctga atctccacgg tttccagcac ttcgagcagt gtcggaccgc
    66361 tgtaccacgg catactttca ctttgcgaag ccacgttgtc accttccagt gcggagagcg
    66421 gcacaaagcg gatatccaga ttacccggca gctgcccggc aaaggtcaga taatcttcac
    66481 gaatacgggt gaacgtctct tcactgtaat ccaccagatc cattttgttg atcgccacga
    66541 ccagatgttt gatccccaac agtgtggaga taaaactgtg acgacgggtt tgatcgagca
    66601 cgcctttacg ggcatcgatc agtaagatcg ccagttcaca tgtcgatgcg ccagtcgcca
    66661 tattgcgggt gtactgctcg tgccctggag tgtcggcgat aataaattta cgcttctcgg
    66721 tagagaaata gcggtaggcc acgtcaatgg tgatgccctg ttcacgctca gcttgcaggc
    66781 cgtccaccag cagagccaga tccagctttt cgccctgggt gccgtgacgc ttactgtcat
    66841 tatgcagcga tgagagctga tcttcataga tttggcgggt atcgtgcaac agacgaccaa
    66901 tcagggtact tttgccgtca tcgacgctac cacaggtcag aaaacgcagc aggcttttat
    66961 gttgttgcgc aatcatccag gcttcgacgc cgccttcatt ggcgatttgt tgtgcaagtg
    67021 cggtgttcat cttaaaaata cccctgacgt tttttcagct ccatagaacc agcttggtcg
    67081 cggtcaatca cgcgcccctg acgttcactg gtggtggaaa ccagcatctc ttcgatgatc
    67141 tccggcagcg tttgtgcatt tgactccacc gcaccggtca gcggccagca gcccagcgta
    67201 cggaaacgca ccatccgttt tttaatcact tcgcccggtt gcaggtcgat acggttgtca
    67261 tcaatcatca tcaacatacc gtcgcgttcc agaaccggac gttccgcagc gagatacagc
    67321 ggcacaatgt cgatattttc cagccagatg tattgccaga tatcctgctc ggtccagtta
    67381 gagagcggga aaacgcggat gctttcgcct ttgttaatct gcccgttata gttgtgccac
    67441 agctccgggc gctgattttt cgggtcccag cgatggaagc gatcacggaa agagtagata
    67501 cgctctttag cacgggattt ctcttcgtcg cggcgcgcac caccgaaggc ggcatcaaaa
    67561 ccgtatttat tcagcgcctg cttcaggcct tcggtcttca taatatcggt atgtttcgcg
    67621 ctgccgtgca cgaatggatt aatccccatc gccacgcctt ccgggttttg atgcaccagc
    67681 agctcgcagc cgtaggcttt cgccgtacga tcgcggaact catacatctc acggaatttc
    67741 cagccggtat cgacatgcag caacgggaaa ggcagcgtac ctggataaaa cgccttgcgc
    67801 gccagatgca gcatgacgct ggaatcttta ccgatagagt agagcatcac cggatttgag
    67861 aattctgccg ccacctcgcg aataatgtgg atgctttccg cctccagttg ccgcaggtga
    67921 gtaagtcgta tttgatccat aaccgttcct ttgcaatacc gctattttct tgccatcaga
    67981 tgtttcgact atagggagcg taagagaacg aatgaaatta ccaattagaa tgagtagttc
    68041 cttaacggaa taacgatttg gcaaagctaa tatcaaaaag tgcttaaggc accggatttc
    68101 gggcgtttag gaagatttga aattgtttta gcgcagcggc agtttcatac tatggcggta
    68161 aaaaaatttg catggtattt aaggactcac tatgttttcc gcattgcgcc accgtaccgc
    68221 tgccctggcg ctcggcgtat gctttattct ccccgtacac gcctcgtcac ctaaacctgg
    68281 cgattttgcc aatacacagg cgcgacatat tgccactttc tttccgggac gaatgaccgg
    68341 aacacccgca gaaatgttat ctgccgatta tattcggcaa cagtttcagc aaatgggtta
    68401 ccgcagtgat attcgtacgt ttaatagccg atatatttat accgcccgcg ataaccgcaa
    68461 aaactggcac aacgtgacgg gaagtacggt gattgccgct catgaaggca aagcgccgca
    68521 gcagatcatt attatggcgc atctggatac ctatgccccg cagagcgacg cagatgcaga
    68581 tgccaatctc ggcgggctga cgttacaagg aatggatgat aacgccgcag gtttaggtgt
    68641 catgctggaa ctggcagaac gcctgaaaaa tacgcctacc gagtatggta ttcgatttgt
    68701 ggcgaccagt ggagaagagg aagggaaatt aggcgctgag aatttactca agcggatgag
    68761 tgacaccgaa aagaaaaata cgctgctggt gattaatctc gataacttaa ttgttggcga
    68821 taaattgtat ttcaacagcg gtgtaaaaac ccctgaagca gtaaggaaat taacgcgcga
    68881 cagggcgctg gcaattgcgc gtagtcatgg aattgccgca acgaccaatc cgggtttgaa
    68941 taaaaattat ccgaaaggca ctggatgttg taatgacgca gaaatattcg acaaagcggg
    69001 cattgctgta ctttcggtgg aagcgacaaa ctggaatctt gggaataaag atggttatca
    69061 gcaacgcgca aaaacagccg cattccctgc gggaaatagc tggcatgacg taagactgga
    69121 taatcagcaa catattgata aagcacttcc tggaagaata gaacgtcgct gccgtgacgt
    69181 tatgcggata atgctaccgc tggtgaagga gttggcgaag gcgtcttgat gggttggaaa
    69241 atgggagctg ggtgttctac cgcaggggcg gggaattcta agtgatatcc atcatcgcat
    69301 ccagtgcgcc cggtttatcc ccgctgatgc ggggaacaca gcggcacgct ggattgaaca
    69361 aatccctggg ccggtttatc cccgctggcg cggggaacac aaaccgaaac acacgatcaa
    69421 tccgaatatg agcggtttat ccccgctggc gcggggaaca ctttggtgac agtttttgtc
    69481 actgttttgg tgacggttta tccccgctgg cgcggggaac acactaagca tacatatctg
    69541 tttttaaaca aatttattcc acatcaacaa tctaccaact aaattcaaac atttccttat
    69601 ttttaaagaa cacataacct attgattatc aacaggaaga aaagaaacca aacgtaaccc
    69661 atccaaatcc accggaatac gtctgttttc tccccaggtc tgaaattcaa aacccgactc
    69721 ggtattggtc gcccaggcca tcaccacatt tccgcaacca gccagttggg taatttgctg
    69781 ccagatcatc tcccgaatac gttttgatgt atcaccaaca tacacaccgg cacgcacttc
    69841 cagtagccag attgcgagcc gtccacgtaa gcgcggaggg acattttctg taacaaccac
    69901 gaccatgctc atccgccgcg cccccggtga ccactatcac ccagcgtttc aggttcaggg
    69961 atggcaggcg gtaacatatc cggcgcgggt tgtggtggtt caatttcacc tgcagcaagg
    70021 actttctcaa ttaacggtat taatttgccc gttaacttag tgctacggaa aatatcgcga
    70081 caggctaatc tgacttcttt atcaggttct gcgggttgcc tcgctgctat ttcaaatgcc
    70141 tttggcacaa ccgaatcaaa tttaatgata tcggctatgt cataaacaaa tgaaagcggt
    70201 ttgccactat gaataaatcc aatagcgggc gcatatcccg cggctaatac tgccgcttca
    70261 gaaataccgt acagacatga tgtggcagca ctgatgcagc gattcacaac atcgcctttt
    70321 tcccagtctt taggatcgta tttgcgacca ttccatttca caccatattg tttcgccagt
    70381 aatgcatagg tctggcgaac gcgggatccc tcaattcccc gtagctgatc cactgaacgg
    70441 cgagctggcg gtggctcacg aaaacgtaat tcatacattt tgcgcaccac cttcaggcgt
    70501 agatcttccg ttaaagccag ctttgcctgg tagagtaatt tatctgcccg cgcccctccg
    70561 ggttgtccgg aagagtaaac gcgaacgccc gcttcaccga cccagaccag cagtgttccc
    70621 accgtggcgg ccagatgcac cgccgcgtgg gaaactctcg ttcccggttc gagcataatg
    70681 caggcgaccg atcccaccgg aatgtgcgtg cggatcccgg ttttgtcgat cagcacgaaa
    70741 gcgccgtcca gtacgtcgat ttgaccgtac tggaggaaga tcatagaggt gcgatctttt
    70801 aacgggatcg gactcagtgg tacaaacgtc acacctctgc tccgggtttg atcagcatca
    70861 gaccacagcc gaacgcgcgg cttttgccat acccctgact gagacgttgc agaaataacc
    70921 cgggatcggt gaccgtaagc atccccgtat aatccacgct actgaactgg atcagttgcc
    70981 gggagttttc ccgccgcagt tgctgttgtc ggtaggcatc aaccgaggta tccagcagtg
    71041 taaaaccgct tctctccccc tgcgctgcca gccagtccag cgccgcctgt tgttgatgca
    71101 accagacatc acttccttcc gcctgccccc tcacctgccg tttcgcctcc atcagcagat
    71161 cgtggcgctt gcccgcttta cagattgttg gattcgcccg caggttgaaa caaagttgtt
    71221 gtccggtacg cagctcgggc acaaatgacc ggcattcgat ggtgaacgtt tcactttccg
    71281 ccgggcgttc ctgcgacagt acaaaaaagc gaaacgcgcc ctggagttct tcccgacgat
    71341 aaagaaattg cctttctttg ccgccaggga agagatccca cagccactga tgcatcacat
    71401 attccccgcg atccaccaaa tgcagcaact gcgcaggcga aagctggccg gtatgtaagg
    71461 ttattcttga gaggtacatg gttcctcctt gctgagccac ggcccctgat tgatggtgcg
    71521 ttccccaaac agccactgct gacgatttaa aggaacatcc cggcgacgta atattttgct
    71581 cgcgaccagg ccgtcgtgtt ccccttccca ccagcattca tcctgaagtt tcgggagtga
    71641 gactttcagt tcgcggaaac tatcctgata ctgttggtat gcgttacgta agacatcaga
    71701 cgcgttgcct tcgagcagta acggcgcaag tggtaacgcc agcggatgac tttttcgccc
    71761 cagataaagc ggaaaaaccg gatgacgtaa accgtcctgc aactgttcaa ggctgtaagg
    71821 cgcatcgggg gttgttgcca ccgccaccat ccaccaggca tcggtgtagt agtcgcgccg
    71881 ggagataatc gcgctcagaa gatcaggggc gctcaactct tcgcgacggc tgaaataacg
    71941 cgctttacgc acctcttttg gcatctggac cgtgtgataa tcccgtgccc agcgcgggtt
    72001 acggctggcg caaaccacca gtgaatagtg gcggttaaac gcgtttaatc gttcggtatc
    72061 atcacgccga atccctaccc cggcagccag cagccccagc aatgctgagc gcgaaggcag
    72121 ttcatgggta tgacgcactt cgccgggggc atcgacgccc caggatgcca ttggcccatg
    72181 aagctgaaaa atcaaatatt ggctcattag ccgcccctta cgcgcagata aagtccagca
    72241 cgtccttcat gcttccctgt ttgttcatca cgtcaaagct tgcgcattcg gtcttctgtt
    72301 catagaccgt attcatattt tcgcgaagcg ttgtaatacg ctgcaccgcc acatctaact
    72361 gccgggtgcc attaatgggt tcatagaaag ccgccgccag agaacgtggt tgttcggtgc
    72421 ctttttctgc cagcgcccag gaggcgtagg cacggctggc aaagctgttc tgtttgccgg
    72481 ttggggagac tttaagtgcg gcttccgtaa aggcgcgcaa ggtctgatta gctaacgctt
    72541 cgtcaccgcc gaggttttcg accagcagat ctttatcgat gcagatatag gtgtagaaca
    72601 gcgcagaacc gaatccggtt tcaccaagat gcccggcacc ggcatcttca gaagcctggc
    72661 gcaaatcatc aacggcggtg aaaaaatcat cttcgacaat cgtttcactg acaccaaatg
    72721 catgcgcgac ctggcaggcg gcttcaacat taaattcggg tttattcgcc agcatacgac
    72781 caaacatagc gatatctact gccatgcgat ctttacgtaa caaagcgaga tcttcctctt
    72841 ttggcgcgcg cttttcttcg gccagttgat gggccagcgc ttttacggcg tcaaattctg
    72901 ccgggctgat atggactaat tgttcagttt cggcgttagt gagcggatct tttggttttt
    72961 tgtcgttttt agctttccca agataatccg caatttttgc cgcccattcg atggcttttt
    73021 tctcttcgat gcctttctca atcaggatag ttgccgcctc acgcgcaata cgcccactgc
    73081 gaataccaat atggcccgcc agtgcctgtt caaaaagtgc agaagtgcgc cacgcacgtt
    73141 tcagactttg cgaggaaacg cgcagtcgcg ttgctccacc caggaccacg gttttcggcg
    73201 ctccggtgtc atcacggtta aggttggctg cagggtaagc ggttaacaaa tgaagctgaa
    73261 taaatgtcgt catgagatag tcctttattg attttgttcg ttatcgacgt cgccagcctg
    73321 gtaatattcc agcgcccagc ggatacggat aaattcggtt ggccgctgat gacggcggtg
    73381 atgattgagc agatcgtcgc tctcctggca ccagcggaag acaccctctg ccagagagtc
    73441 aagattaacg gaaccgttta ataatctgac tacgcggcgt aactggcgca gtaattcatc
    73501 cggtgttttt actgccgaca ggcgagtaaa acgccccttt gacattaccg ccgccagctg
    73561 cgcagcaaaa ggcagccgtt catcaatggc tttaacatta gcgctaagtg cggctatcag
    73621 cgccagtgcg gtaatacgcc attcaggttc atcctgccac tttatttgtc tgttcttcag
    73681 aaacaggcga aatccatccg tcagacaaac atcattaacc gtcgtactac gccgcagact
    73741 ggcacgttcg ccgcgtttct cctgcaattc ctcatgccat ttgcgcagcg tggctttgtg
    73801 ctcctctttt acaatactca ttcagcagcc tcctgctttt tctccctggc ggctttagca
    73861 ctttgcttct ccgccgatgt tgtaaaatat ttcttgcgcg cggtcatgac gcgttccaaa
    73921 tcaacgggct cataaggatt ggtgaataca cgctcgtcaa aatcctgacg tgcgaataac
    73981 caaatttcct tttgccattt gccgagtaat tcatccgcat cctgaccttc ttcaatttgg
    74041 cgcactaacc tcaggaagcg atgctgagtt ttgttccaga agtcgatatc cacaaaactg
    74101 aaatcacccc ttgcaccttt tggatcggag aaccatgctt ctttcaatgc actccgtaac
    74161 agactcagaa tccgtgaagc cgtttgcgca gccagccgca gcttcggtat ctggccttct
    74221 tttttattga gcagcagcgg gaaatggtgt tcgtaccaac agcgcgcttt catgttgtcg
    74281 aaatcataac caaatcccca caggcccact tttgcctgtt tcagactgct ggcattaaag
    74341 agtttcacca ccagcgcggg aagttccgta ttgttttctg acttacccgt ttcgataagg
    74401 cctaaccagt cgcgccagat taaaccgccc ggttgtggtt taacggagta aaactcaccg
    74461 ccctctttaa gtggtacacg gtaaggcgtt aagggatgct gccacatggc ataattcgca
    74521 ccgtaatttt tggtagtcat caaactcaga agcgcgtcac tctgctcacc gcaaatatcg
    74581 cagttgccga ctgtcgtggt attaaaatca atacgaatac gccgcggcat tccccagtac
    74641 gcctggagtt tattgacctg atcatcggtt accaccgcac cggccagttc gctggtacgc
    74701 gtcgggccaa gccaggggaa aaccagatcg tcaaattttt tgggtagcgg taagtcggct
    74761 tcatcctgcg gcatcacgtt gagccacagt ttgcgccaca agggggcttg ttgattgccc
    74821 tgatactcct gcaattcaat cagagtcgtc atcggcccac cgccgcgtaa accggtgcga
    74881 tagcctttgc cacctgacgg cgcatttaac tgtagggaga acagagctaa cgcagaacaa
    74941 tgagagcata cgtgttcagt cacgccacgc ttaataaagt ggtctttatt aaacttcgtt
    75001 gtttgagcgc cgggaatctc aggcagtagc gaagcgacct gaactttatc gcccatgagc
    75061 acctcgaaat cctgcataaa tgaaggtgaa tctgggccaa actggaaagg gtgttctaat
    75121 gacagcaatg cttcccgtag cttttcagct tccagcccgt cttcccagat atcatcccaa
    75181 cgacgataat cttttggcgc gaaactgctt tgtagtaacc ccagcaaaaa ctgccatgcc
    75241 gccccctgga gatctgcccg cggcgcagcg atatcgacaa cattttcatc cgccagatcg
    75301 actggcgcca gcttgcctgt tgttccgtct ttaaaacgaa cgggcaacca cggggttgtc
    75361 agaagtgaaa acgagttcat atgtatcctc tcgtaaaaaa gaccatccct ggtcggttgt
    75421 ccatcattcc atccttttcg gtgtatcaac tattcagaga gtcctgtcgc aggtatctca
    75481 atcaaccttg ccaatcaatc cctccctggc cgaatacccg caactttcgt catcagtgac
    75541 taaaatcacg tttgccattt ccggatcttg ccgctgttca atgcaccact gcctgaacgc
    75601 ttccccttcc agtaaagaaa actcatcccg atgttttttc caccagcttc gacgcactct
    75661 gacaacgctc atttcccatg cgtgagcacc ggtggcataa ggcttcacca caccggcaat
    75721 acaggtagcc agccacaggg aaacagattc ctcagccaga cgtgtcgaca gcttttccgg
    75781 aaggtaatcg ttgatattgg cggcatagcc aggcttgaag ttcaggacaa actttttagc
    75841 cattgcgcga tcgcagtaat atttgcccac ttgctcctgc tcgctgcggg caaatccttc
    75901 cggcattacc acgtcctcac cgtagactga ttcaataaga aggcgggctg cgtgtggcat
    75961 ttgaatagcg ccttgctcac gcagtacacg ctgcgtcagc cagattcgtc catgatcggg
    76021 atagacatat gcactgttac gcatggcact gccgaaccat tcgtcaccag gagcgtcgtc
    76081 ccagacgggg gccagaatca gcaattcagg aggggaacgc tcgtcttttc cgtcacgctt
    76141 taactgacca ttaatatcgc ggatatgccg ctgtaatcgc cccgctcgct gaatcagcaa
    76201 atcaacaggg gccaggtcgg agatcatttc gtccaggtca caatcaacgc tctgctctaa
    76261 gacctgagta caaatgagga cttttccggc acgctgtgaa ccgtcttctt taccaaagcg
    76321 tgccagcgtc tccatttcaa ttcgctggcg atcgctaaaa gcaaagcggc tatgaaagag
    76381 tgaaaggctg gaagcgggaa tgacgccgcg ggcaagcagc tgacgatgaa ccttaatagc
    76441 gtcatcgaca gaattccgga tccaggcgat gcattttccc tgacttaccg ccgattcgat
    76501 acgcgcaata ctctcttgtt cactatgaag ccaacccacg ctgacgctac gctcaacgtc
    76561 tttgcgcgtc gctacccggt gtgagttcac atcggatttc gtgacatgcg tcagccaggg
    76621 gtaatcatcc ttttcaagga acggagcttc ttgctggccc tctgtgccac gcgcaaaggc
    76681 ggcgacgagt ttgtcgcgct gctgttggga taacgtagca gaaagcaaaa tgacgctgtt
    76741 tccgccacgc gcctgccgct cgatcagccc ttcaagaatg cacgacatgt aagcatcaca
    76801 ggcatggatc tcatcagcca gcaggatttt gttactcaac cccagaagcc gcagattatt
    76861 atgtttaaac ggcatcactg ccatcatcgc ctgatccagc gtgccgacgc caatttcagc
    76921 cagtagcgcc ttcttgttac tgttggcaaa ccaggccgca catccctgac tgaatgtttg
    76981 ttcatccggt tcttctgacc cgactaaatc accggaccag agtgattcat tgaagcggtc
    77041 cattaatgtg cgggcactgt gtgccagcac caagctgggg cgggactctg gcgaatagaa
    77101 agcaagccag gttttgacca gccgatcgta catggcattg gccgttgcca ttgttggcag
    77161 gccaaaaaac aaaccctgtg ctttcctcgc agccatcaac ctgtgcgcca ggataagcgc
    77221 cgcttctgtt ttacctgcgc cagtcacgtc ttccagaata aataactgtg gccctggctg
    77281 gctgatatcc agatccagta ccttttgctg taatggtgtc gggtgctcaa taaaaggaaa
    77341 cagcgtatta attccggtga aaggtgcggt ttctgctttt ggaggaaaga cggttaaggc
    77401 gttttgagcc tgaactaaag ttttctgcca gtaatcttta atatccattg ggtgtgcgac
    77461 gcgtggaaaa aatcgcgttg acgaacccgt ccagtctgcg agtacgactg ttgcagagat
    77521 ataccaggaa agttgtttta aaagttcaac gccctcgtca tcatcccaga atgtgggaat
    77581 ctctatgagc ggaaacagtg ccttgatttc aaggagaaaa tctcgcgcgg cagctttgtc
    77641 ttcaggcaga aaattatcca gctcatcaat acggtcaggt ggtcgaccat gatgcccggt
    77701 agttatggac atccacatct ctattacacg tgtaagttta cgagaagaga gtgaagatga
    77761 aggaagcaac tcctcacatt cacttaaata ataattccac agccagtaac ccagcgttga
    77821 atgagagatc ttttcgtaat tctttctgga accttccgga atcttgagtt caggggccag
    77881 gtaaagttgc tgaaaagagc gggcaaattt tccaatatcg tgccagcaca gcaaccaagc
    77941 gaaaaattga gccgcctgtt ccttgtcaga aatccctaat tgacgaaagt aatcagccag
    78001 cccgaagcaa tttcttt
//
LOCUS       DBFIFI010000003        76212 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.003, whole genome shotgun sequence.
ACCESSION   DBFIFI010000003 DBFIFI010000000
VERSION     DBFIFI010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 76212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.003"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            131..757
                     /gene="ydhY"
                     /locus_tag="N7B39_000157"
     CDS             131..757
                     /gene="ydhY"
                     /locus_tag="N7B39_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HGU2420220.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            778..2880
                     /gene="ydhV"
                     /locus_tag="N7B39_000158"
     CDS             778..2880
                     /gene="ydhV"
                     /locus_tag="N7B39_000158"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HGU2420221.1"
                     /translation="MTNGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAECWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPA"
     gene            2893..3531
                     /gene="ydhW"
                     /locus_tag="N7B39_000159"
     CDS             2893..3531
                     /gene="ydhW"
                     /locus_tag="N7B39_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HGU2420222.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSVAGQLVARAVFLSPPYSVAEEELSVLLENIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            3595..4263
                     /gene="ydhX"
                     /locus_tag="N7B39_000160"
     CDS             3595..4263
                     /gene="ydhX"
                     /locus_tag="N7B39_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310405.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HGU2420223.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICACACRKTNHAPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            4260..5045
                     /gene="phsC"
                     /locus_tag="N7B39_000161"
     CDS             4260..5045
                     /gene="phsC"
                     /locus_tag="N7B39_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HGU2420224.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGERIYLYSKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINGVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            5049..5861
                     /gene="ydhT"
                     /locus_tag="N7B39_000162"
     CDS             5049..5861
                     /gene="ydhT"
                     /locus_tag="N7B39_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HGU2420225.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGERAFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGKRCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRAGVRYRLNEQHQFVEC"
     gene            complement(5873..7477)
                     /gene="ydhS"
                     /locus_tag="N7B39_000163"
     CDS             complement(5873..7477)
                     /gene="ydhS"
                     /locus_tag="N7B39_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HGU2420226.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTLLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPIYCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARKQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EYGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDQFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(7603..7908)
                     /gene="ydhR"
                     /locus_tag="N7B39_000164"
     CDS             complement(7603..7908)
                     /gene="ydhR"
                     /locus_tag="N7B39_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HGU2420227.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTAQLKKLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(8016..8092)
                     /locus_tag="N7B39_000165"
     tRNA            complement(8016..8092)
                     /locus_tag="N7B39_000165"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8056..8058),aa:Val,seq:gac)
     gene            complement(8097..8173)
                     /locus_tag="N7B39_000166"
     tRNA            complement(8097..8173)
                     /locus_tag="N7B39_000166"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8137..8139),aa:Val,seq:gac)
     gene            8481..9737
                     /gene="ydhQ"
                     /locus_tag="N7B39_000167"
     CDS             8481..9737
                     /gene="ydhQ"
                     /locus_tag="N7B39_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HGU2420228.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKANDTLLADGTMNNLGGEDSDTIVENGAIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTLTVQQGGVLILDGSTVKGDSVTFSIGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVQITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(9778..11151)
                     /gene="mdtK"
                     /locus_tag="N7B39_000168"
     CDS             complement(9778..11151)
                     /gene="mdtK"
                     /locus_tag="N7B39_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HGU2420229.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRYLQRLPSAI
                     ILQRASR"
     gene            11366..12007
                     /gene="ribE"
                     /locus_tag="N7B39_000169"
     CDS             11366..12007
                     /gene="ribE"
                     /locus_tag="N7B39_000169"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HGU2420230.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQSGTEA"
     gene            complement(12047..13195)
                     /gene="cfa"
                     /locus_tag="N7B39_000170"
     CDS             complement(12047..13195)
                     /gene="cfa"
                     /locus_tag="N7B39_000170"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HGU2420231.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFTVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFVAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(13486..14697)
                     /gene="punC"
                     /locus_tag="N7B39_000171"
     CDS             complement(13486..14697)
                     /gene="punC"
                     /locus_tag="N7B39_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HGU2420232.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAAMLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            14810..15742
                     /gene="punR"
                     /locus_tag="N7B39_000172"
     CDS             14810..15742
                     /gene="punR"
                     /locus_tag="N7B39_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HGU2420233.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(15739..16764)
                     /gene="purR"
                     /locus_tag="N7B39_000173"
     CDS             complement(15739..16764)
                     /gene="purR"
                     /locus_tag="N7B39_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HGU2420234.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            17063..17152
                     /gene="cydH"
                     /locus_tag="N7B39_000174"
                     /gene_synonym="ynhF"
     CDS             17063..17152
                     /gene="cydH"
                     /locus_tag="N7B39_000174"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HGU2420235.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            17318..18487
                     /gene="ydhP"
                     /locus_tag="N7B39_000175"
     CDS             17318..18487
                     /gene="ydhP"
                     /locus_tag="N7B39_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2420236.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNKFGAAISMAVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(18633..19214)
                     /gene="sodB"
                     /locus_tag="N7B39_000176"
     CDS             complement(18633..19214)
                     /gene="sodB"
                     /locus_tag="N7B39_000176"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HGU2420237.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19342..20157)
                     /gene="mepH"
                     /locus_tag="N7B39_000177"
     CDS             complement(19342..20157)
                     /gene="mepH"
                     /locus_tag="N7B39_000177"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HGU2420238.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHTVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20492..20839
                     /gene="grxD"
                     /locus_tag="N7B39_000178"
     CDS             20492..20839
                     /gene="grxD"
                     /locus_tag="N7B39_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HGU2420239.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(20890..25506)
                     /gene="lhr"
                     /locus_tag="N7B39_000179"
     CDS             complement(20890..25506)
                     /gene="lhr"
                     /locus_tag="N7B39_000179"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HGU2420240.1"
                     /translation="MADNPNPSSPLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARG
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLKGIADERRRRGETEVNLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASGTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAADAAHFE
                     STSGATSNRVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVELVIQVATPLSVASGLQRIGRAGHQIGGISKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQHTVAAAAMDALQVDEWYSRVRRAAPWKDLPRRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEQA
                     GSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTIANIQGLIDEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARALLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPAILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEIASYLE
                     NLLTVKRIFPTMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVHDYSPEVLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEADQANRSALQQAIVAVLADGGAWFAQQISQRIRDKIGESVDPSALQEAL
                     WALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHRARRGRPVYAQPVSPRVSYNTPN
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQAAQTRHYTPVALSANDPANVWGNL
                     LPWPAHPATLVPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEVFHALTT
                     ALRREPRLRFTLTEVNDLPVWQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(25599..26246)
                     /gene="rnt"
                     /locus_tag="N7B39_000180"
     CDS             complement(25599..26246)
                     /gene="rnt"
                     /locus_tag="N7B39_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HGU2420241.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(26349..26755)
                     /gene="gloA"
                     /locus_tag="N7B39_000181"
                     /pseudo
     CDS             complement(26349..26755)
                     /gene="gloA"
                     /locus_tag="N7B39_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
     gene            complement(26836..27933)
                     /gene="nemA"
                     /locus_tag="N7B39_000182"
     CDS             complement(26836..27933)
                     /gene="nemA"
                     /locus_tag="N7B39_000182"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HGU2420242.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPSI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(27970..28569)
                     /gene="nemR"
                     /locus_tag="N7B39_000183"
     CDS             complement(27970..28569)
                     /gene="nemR"
                     /locus_tag="N7B39_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HGU2420243.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYATYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRDSHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRNYEPLENALAHVKTIIATPAV"
     gene            28672..28911
                     /gene="ydhL"
                     /locus_tag="N7B39_000184"
     CDS             28672..28911
                     /gene="ydhL"
                     /locus_tag="N7B39_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HGU2420244.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            28960..29856
                     /gene="ydhF"
                     /locus_tag="N7B39_000185"
                     /pseudo
     CDS             28960..29856
                     /gene="ydhF"
                     /locus_tag="N7B39_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
     gene            29937..30458
                     /gene="sodC"
                     /locus_tag="N7B39_000186"
     CDS             29937..30458
                     /gene="sodC"
                     /locus_tag="N7B39_000186"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HGU2420245.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(30459..32471)
                     /gene="ydhK"
                     /locus_tag="N7B39_000187"
     CDS             complement(30459..32471)
                     /gene="ydhK"
                     /locus_tag="N7B39_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HGU2420246.1"
                     /translation="MNASSWSLRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETTDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREIL
                     EQLLTALASSQTDVYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSDVDDHARLPRTSGLARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYNFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGIVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(32471..33328)
                     /gene="ydhJ"
                     /locus_tag="N7B39_000188"
     CDS             complement(32471..33328)
                     /gene="ydhJ"
                     /locus_tag="N7B39_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HGU2420247.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVDAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(33331..33567)
                     /gene="ydhI"
                     /locus_tag="N7B39_000189"
     CDS             complement(33331..33567)
                     /gene="ydhI"
                     /locus_tag="N7B39_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HGU2420248.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            33768..34202
                     /gene="slyA"
                     /locus_tag="N7B39_000190"
     CDS             33768..34202
                     /gene="slyA"
                     /locus_tag="N7B39_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HGU2420249.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(34249..34716)
                     /gene="slyB"
                     /locus_tag="N7B39_000191"
     CDS             complement(34249..34716)
                     /gene="slyB"
                     /locus_tag="N7B39_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HGU2420250.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            34989..36098
                     /gene="anmK"
                     /locus_tag="N7B39_000192"
     CDS             34989..36098
                     /gene="anmK"
                     /locus_tag="N7B39_000192"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HGU2420251.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVKALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            36196..36525
                     /gene="mliC"
                     /locus_tag="N7B39_000193"
     CDS             36196..36525
                     /gene="mliC"
                     /locus_tag="N7B39_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HGU2420252.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGLSASGARYSDGIYVFWSKGEEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            36584..37240
                     /gene="pdxH"
                     /locus_tag="N7B39_000194"
     CDS             36584..37240
                     /gene="pdxH"
                     /locus_tag="N7B39_000194"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HGU2420253.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGSWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            37369..38643
                     /gene="tyrS"
                     /locus_tag="N7B39_000195"
     CDS             37369..38643
                     /gene="tyrS"
                     /locus_tag="N7B39_000195"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HGU2420254.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            38705..39565
                     /gene="pdxY"
                     /locus_tag="N7B39_000196"
     CDS             38705..39565
                     /gene="pdxY"
                     /locus_tag="N7B39_000196"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HGU2420255.1"
                     /translation="MKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNHT
                     QYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKAA
                     NPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHPVNNV
                     EEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQP
                     VGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIAK
                     PEHYFSATKL"
     gene            complement(39609..40214)
                     /gene="gstA"
                     /locus_tag="N7B39_000197"
     CDS             complement(39609..40214)
                     /gene="gstA"
                     /locus_tag="N7B39_000197"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HGU2420256.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(40320..41822)
                     /gene="dtpA"
                     /locus_tag="N7B39_000198"
     CDS             complement(40320..41822)
                     /gene="dtpA"
                     /locus_tag="N7B39_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HGU2420257.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQNGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHTILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(42433..43068)
                     /gene="nth"
                     /locus_tag="N7B39_000199"
     CDS             complement(42433..43068)
                     /gene="nth"
                     /locus_tag="N7B39_000199"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HGU2420258.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNS
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(43068..43763)
                     /gene="rsxE"
                     /locus_tag="N7B39_000200"
     CDS             complement(43068..43763)
                     /gene="rsxE"
                     /locus_tag="N7B39_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HGU2420259.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(43767..44387)
                     /gene="rsxG"
                     /locus_tag="N7B39_000201"
     CDS             complement(43767..44387)
                     /gene="rsxG"
                     /locus_tag="N7B39_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HGU2420260.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGNFDQFTGATITPRAVVNAVKRAGLYAQTLPEQLSQLPACGE"
     gene            complement(44391..45449)
                     /gene="rsxD"
                     /locus_tag="N7B39_000202"
     CDS             complement(44391..45449)
                     /gene="rsxD"
                     /locus_tag="N7B39_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HGU2420261.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(45450..47768)
                     /gene="rsxC"
                     /locus_tag="N7B39_000203"
     CDS             complement(45450..47768)
                     /gene="rsxC"
                     /locus_tag="N7B39_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HGU2420262.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRSRRREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSHDISMRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVTDPRKTAVEAAIARA
                     KARKLEQQQANAEPEQQVDPRKAAVEAAIARAKARKLEQQQANAEPEQQVDPRKAAVE
                     AAIARAKARKLEQQQANAEPEEPVDPRKAAVEAAIARAKARKLEQQQANAEPEEPVDP
                     RKAAVEAAITRAKARKLEQQQANAEPEEQVDPRKAAVAAAIARAKARKLEQQQANAEP
                     EEQVDPRKAAVAAAIARVQAKKAVQQKVVNED"
     gene            complement(47761..48339)
                     /gene="rsxB"
                     /locus_tag="N7B39_000204"
     CDS             complement(47761..48339)
                     /gene="rsxB"
                     /locus_tag="N7B39_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HGU2420263.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(48339..48920)
                     /gene="rsxA"
                     /locus_tag="N7B39_000205"
     CDS             complement(48339..48920)
                     /gene="rsxA"
                     /locus_tag="N7B39_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HGU2420264.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(48997..49437)
                     /gene="ydgK"
                     /locus_tag="N7B39_000206"
     CDS             complement(48997..49437)
                     /gene="ydgK"
                     /locus_tag="N7B39_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HGU2420265.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGAQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(49523..49738)
                     /gene="ydgT"
                     /locus_tag="N7B39_000207"
     CDS             complement(49523..49738)
                     /gene="ydgT"
                     /locus_tag="N7B39_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HGU2420266.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(50011..50136)
                     /gene="blr"
                     /locus_tag="N7B39_000208"
     CDS             complement(50011..50136)
                     /gene="blr"
                     /locus_tag="N7B39_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HGU2420267.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSTTVQHK"
     gene            50379..51419
                     /gene="ydgJ"
                     /locus_tag="N7B39_000209"
     CDS             50379..51419
                     /gene="ydgJ"
                     /locus_tag="N7B39_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HGU2420268.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAIMLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(51455..52456)
                     /gene="add"
                     /locus_tag="N7B39_000210"
     CDS             complement(51455..52456)
                     /gene="add"
                     /locus_tag="N7B39_000210"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HGU2420269.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLNAEEKRALREKVAAK"
     gene            complement(52560..53732)
                     /gene="malY"
                     /locus_tag="N7B39_000211"
     CDS             complement(52560..53732)
                     /gene="malY"
                     /locus_tag="N7B39_000211"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HGU2420270.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDPQSVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYIADKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(53742..55334)
                     /gene="malX"
                     /locus_tag="N7B39_000212"
     CDS             complement(53742..55334)
                     /gene="malX"
                     /locus_tag="N7B39_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HGU2420271.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLLPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVVAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            55509..56537
                     /gene="malI"
                     /locus_tag="N7B39_000213"
     CDS             55509..56537
                     /gene="malI"
                     /locus_tag="N7B39_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HGU2420272.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLVHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            56649..57416
                     /gene="hdhA"
                     /locus_tag="N7B39_000214"
     CDS             56649..57416
                     /gene="hdhA"
                     /locus_tag="N7B39_000214"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HGU2420273.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            57645..58235
                     /gene="uidR"
                     /locus_tag="N7B39_000215"
     CDS             57645..58235
                     /gene="uidR"
                     /locus_tag="N7B39_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HGU2420274.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            58626..60439
                     /gene="uidA"
                     /locus_tag="N7B39_000216"
                     /pseudo
     CDS             58626..60439
                     /gene="uidA"
                     /locus_tag="N7B39_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
     gene            60436..61809
                     /gene="uidB"
                     /locus_tag="N7B39_000217"
     CDS             60436..61809
                     /gene="uidB"
                     /locus_tag="N7B39_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HGU2420275.1"
                     /translation="MNQQLSWRTIVGYSLGDIANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHGSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            61848..63112
                     /gene="uidC"
                     /locus_tag="N7B39_000218"
                     /pseudo
     CDS             61848..63112
                     /gene="uidC"
                     /locus_tag="N7B39_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
     gene            complement(63157..64665)
                     /gene="ydgA"
                     /locus_tag="N7B39_000219"
     CDS             complement(63157..64665)
                     /gene="ydgA"
                     /locus_tag="N7B39_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HGU2420276.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLVKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPVVPAIPQQ"
     gene            complement(64766..65941)
                     /gene="manA"
                     /locus_tag="N7B39_000220"
     CDS             complement(64766..65941)
                     /gene="manA"
                     /locus_tag="N7B39_000220"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HGU2420277.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HKSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPDAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPDDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            66140..67786
                     /gene="fumB"
                     /locus_tag="N7B39_000221"
     CDS             66140..67786
                     /gene="fumB"
                     /locus_tag="N7B39_000221"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HGU2420278.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSLSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQALGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            67929..69332
                     /gene="fumC"
                     /locus_tag="N7B39_000222"
     CDS             67929..69332
                     /gene="fumC"
                     /locus_tag="N7B39_000222"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HGU2420279.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIA
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(69329..70258)
                     /gene="tus"
                     /locus_tag="N7B39_000223"
     CDS             complement(69329..70258)
                     /gene="tus"
                     /locus_tag="N7B39_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HGU2420280.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSQALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(70334..71635)
                     /gene="rstB"
                     /locus_tag="N7B39_000224"
     CDS             complement(70334..71635)
                     /gene="rstB"
                     /locus_tag="N7B39_000224"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HGU2420281.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(71639..72358)
                     /gene="rstA"
                     /locus_tag="N7B39_000225"
     CDS             complement(71639..72358)
                     /gene="rstA"
                     /locus_tag="N7B39_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HGU2420282.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDIQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            72487..72822
                     /gene="ydgC"
                     /locus_tag="N7B39_000226"
     CDS             72487..72822
                     /gene="ydgC"
                     /locus_tag="N7B39_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HGU2420283.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(72819..73541)
                     /gene="folM"
                     /locus_tag="N7B39_000227"
     CDS             complement(72819..73541)
                     /gene="folM"
                     /locus_tag="N7B39_000227"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HGU2420284.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(73578..74960)
                     /gene="ydgI"
                     /locus_tag="N7B39_000228"
     CDS             complement(73578..74960)
                     /gene="ydgI"
                     /locus_tag="N7B39_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HGU2420285.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDIFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(75146..76090)
                     /gene="ydgH"
                     /locus_tag="N7B39_000229"
     CDS             complement(75146..76090)
                     /gene="ydgH"
                     /locus_tag="N7B39_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HGU2420286.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
BASE COUNT        19174 a        18837 c        19802 g        18399 t
ORIGIN      
        1 ttttctcata cactatcggg ttagggaaaa tatctggtgt tggggacaga gtcagcagcg
       61 tattagttga atgcttaata tcaggctgct gaattgtcag cactgtgttt gcgaagagga
      121 tgcgaaataa atgaacccgg ttgatcgtcc actattagat attggcttaa cgcgattaga
      181 attcttgcgt atatcaggaa aaggccttgc tggtctaaca attgcccctg cgttactgtc
      241 acttttgggt tgcaaacagg aagatattga tagcggcaca gtagggttga taaatacacc
      301 caaaggggtg ctggtgaccc agcgagcgcg atgcacgggt tgccaccgct gtgaaatctc
      361 ttgtaccaac ttcaacgatg gctcagtagg gacattcttc tcccgtatca aaatccatcg
      421 caattatttc tttggcgaca acggggttgg ctctggcggc ggcctgtatg gcgatctcaa
      481 ctataccgcg gacacctgcc gtcaatgcaa agagccgcaa tgcatgaacg tctgcccgat
      541 tggtgcgatt acctggcagc agaaagaagg ctgtattacc gtcgatcata aacgttgtat
      601 tggctgtagc gcctgtacta cggcatgccc gtggatgatg gccaccgtaa ataccgaaag
      661 taaaaaatcc tcgaaatgtg tgttatgcgg tgaatgcgca aacgcctgcc caacaggggc
      721 gttaaaaatt atcgagtgga aagatattac tgtgtgaatc ttgcaaagga aaaaatcatg
      781 actaacggtt ggacaggtaa tatattaaga gtcaatctca cgacaggaaa tattaccctc
      841 gaagattcca gtaagtttaa aagttttgtc ggtggcatgg gcttcggcta caaaattatg
      901 tatgacgaag taccgccagg cacgaaacct ttcgatgaag cgaataaatt agtctttgct
      961 accggcccat taactggatc tggtgccccc tgtagttctc gcgtaaatat cacctcactt
     1021 tctactttta ccaaaggaaa tttagtcgtc gatgcccata tgggtggctt ttttgcagcg
     1081 caaatgaaat tcgctggata cgacgtcatt attatcgaag ggaaggcgaa atcaccggta
     1141 tggctgaaga ttaaagatga caaagttagc ctggaaaaag cagatttctt atggggaaaa
     1201 gggacgcgcg caacgacgga agaaatttgt cgcttgacca gtccggaaac ctgtgtggcg
     1261 gctattggtc aggctgggga aaaccttgtt cctctctctg gcatgttgaa tagccgtaac
     1321 cacagcggcg gtgcgggaac tggcgcaata atgggttcga aaaacctgaa agcgattgcg
     1381 gttgaaggga cgaaaggggt caacattgcc gatcgtcagg agatgaagcg tctcaatgat
     1441 tacatgatga ctgaacttat cggtgcgaat aacaaccatg tcgtgccaag tacgccgcaa
     1501 tcgtgggcag agtattcaga tcccaagtca cgctggacag cacgtaaagg gctgttttgg
     1561 ggcgcggctg aaggcggtcc aattgaaacg ggtgaaattc cgccaggcaa tcagaatacg
     1621 gtcggctttc gtacctataa atccgttttt gacttaggtc cggcagcaga gaaatacaca
     1681 gtaaaaatga gcggctgcca ctcttgcccg atccgttgta tgacccaaat gaatattcct
     1741 cgggtgaaag agtttggcgt gcccagcaca ggtggtaaca cttgtgtagc aaactttgtc
     1801 cataccacca tcttcccgaa cgggccgaaa gattttgaag ataaagacga tggtcgtgtg
     1861 attggtaacc tggttggtct gaatttgttc gatgactacg gcctatggtg taactacggg
     1921 cagttgcatc gcgactttac ttattgttac agcaaaggtg tgttcaagcg tgttctgcct
     1981 gctgaagagt atgcagaaat tcgctgggat caactggaag cgggagacgt taacttcatt
     2041 aaagattttt actaccgtct ggcgcatcgt gtgggtgagc tgagtcacct ggctgatggt
     2101 tcatatgcca tcgcagaatg ctggaatttg ggtgaagagt actggggcta cgcgaaaaat
     2161 aaactctggt cgccgtttgg ctatccggtt caccatgcca atgaagcgtc agcgcaggtc
     2221 ggttccattg ttaactgtat gttcaaccgt gactgcatga cgcataccca tatcaacttt
     2281 attggttccg gcttgccatt gaaactgcaa cgtgaagtgg cgaaagaact ttttggttct
     2341 gaagatgctt acgatgaaac caaaaactac acgccaatca atgacgcaaa aattaaatat
     2401 gctaagtggt cgctgttgcg ggtctgtttg cataacgccg tcaccctgtg caactgggtc
     2461 tggccaatga ccgtttcgcc gctgaaaagc cgtaattacc ggggcgatct ggcgcttgaa
     2521 gccaaattct tcaaagcgat cactggcgaa gagatgactc aggaaaaatt agatttagct
     2581 gcagagcgta tctttacatt gcatcgtgcc tacacggtga aactgatgca aaccaaagat
     2641 atgcgtaatg aacacgatct tatctgttcc tgggtattcg acaaggatcc gcagatcccg
     2701 gtctttactg aaggtactga caaaatggat cgtgatgata tgcatgcctc gctcacgatg
     2761 ttctacaaag aaatgggctg ggacccacag cttggttgtc cgacccgcga aacattgcag
     2821 cgtctggggc tggaagatat tgccgccgat ctggcagcac acaatctact gcctgcgtaa
     2881 ggaatgggta agatgaacca tcaggatgaa ttgccgttag cgaaagtcag cgaagttgac
     2941 gaggcgaagc ggcaatggtt gcaggggatg cgtcaccccg ttgatacggt aaccgaacca
     3001 gaaccggctg aaatcctggc agagttcatt cgccagcatt ctgtggctgg gcagctggtg
     3061 gcgcgcgccg tgtttctctc gccaccctat tcagttgcag aagaggaact gtcagttttg
     3121 ctggaaaaca tcaagcagaa cggagactac gcggatatcg cttgtatgac gggaagtcag
     3181 gatgattact attactcaac gcaggccatg agtgaaaact acgcggcgat gtctttgcaa
     3241 gttgtggagc aggacatttg tcgcgccatt gcacacgccg ttcgttttga gtgccagacg
     3301 tatcctcgtc cttacaaagt cgccatgctg atgcaagcgc catattactt ccaggaagcg
     3361 cagattgaag ccgcgatcgc cgcaatggat gttgcacctg aatatgccga tatacggcag
     3421 gtagagtcat cgacggcggt gttgtacttg tttagcgagc gatttatgac ctatggcaag
     3481 gcttatggct tatgcgaatg gttcgaggta gagcagtttc agaacccctg aaatttcagg
     3541 gagatgtcat ggatagggtg gacagttgca gccactgcat taggggataa tcagatgtcc
     3601 ttcactcgac gcaaatttgt tctggggatg ggaacagtaa tcttttttac tggttctgcg
     3661 tcttctctgt tagcgaacac gaggcaagaa aaggaagttc ggtatgccat gattcatgac
     3721 gagtcacggt gcaatggctg taatatttgc gcctgcgcat gccgtaaaac taaccacgcc
     3781 cccgctcagg gaagccgttt atcgatagca cacattccag tcaccgataa cgacaacgaa
     3841 acgcagtatc acttttttcg ccagtcatgt cagcactgcg aagatgctcc gtgcatcgac
     3901 gtatgcccga caggggcgtc gtggcgtgat gagcagggga tcgtgcgggt agaaaaatcg
     3961 cagtgcatcg gctgcagtta ttgcattggc gcttgtccgt accaggttcg ctaccttaac
     4021 cctgtcacta aagtggcaga taaatgtgat ttctgcgctg agtcccgact ggcaaaaggc
     4081 tttccgccca tttgcgtcag cgcttgcccg gaacacgcat tgatctttgg tcgcgaagat
     4141 agccctgaaa ttcaggcgtg gttgcaggat aataaatact atcaatatca gctacctggt
     4201 gccgggaaac cgcatctgta tcgccggttc ggtcaacatt tgattaaaaa ggaaaatgta
     4261 tgaacccgtc gcaacatgct gaacagtttc agagccagtt agcgaactat gtgccacagt
     4321 tcactcccga attttggccg gtgtggttga tcattgccgg agtattgctg gttgggatgt
     4381 ggttggtgct ggggctgcac gccttgcttc gtgctcgtgg cgtgaagaaa tcagccaccg
     4441 atcatggtga gaggatttat ctttacagca aagcggtcag attatggcac tggtcgaatg
     4501 cgttactctt tgtattgttg ctggcaagtg ggctgataaa tcactttgcg ctggtgggcg
     4561 caactgcggt taaaagtctg gttgcggtgc atgaagtttg cggatttttg ttactggcat
     4621 gctggctcgg ctttgttctg atcaatggcg ttgggggtaa tggccaccac tatcgcattc
     4681 gtcgtcaggg ttggctggag cgggcggcaa aacaaacgcg attttatttg tttggcatta
     4741 tgcaggggga agaacatcct ttcccggcaa caacccagtc taaatttaat cccttacagc
     4801 aggttgccta tgttggtgtc atgtatggat tgctgccgtt gttactattg acggggctgc
     4861 tgtgtctcta tccgcaagcc gtgggagatg tgtttcctgg cgtaagatac tggttattgc
     4921 aggcgcattt tgctctggca tttataagcc tcttttttat cttcggtcat ctttatcttt
     4981 gcaccacggg gcgtacgcca cacgaaacct ttaaaagcat ggtcgatggc tatcaccggc
     5041 actaacgaat gatcattaca cgagcagacc tgcgagaatg gcgtatcgga gcggtgatgt
     5101 atcgctggtt cctgcgccat ttcccacgcg gcggtagtta tgccgatatt catcatgcac
     5161 tgattgagga aggatatacg gactgggcgg agagtctggt tgagtatgcg tggaaaaaat
     5221 ggctggcgga tgaaaacttc gcgcatcagg aagtttcctc tatgcaaaaa ctggccacag
     5281 accccggaga gagagcattt tgcagtcagt ttgcccgcag cgacgatcat gctcgcatcg
     5341 gctgttgtga ggataacgcc agaatcgcaa ccgctgggta tgctgcacaa attgccagta
     5401 tgggttatag cgtgcggatt ggaagtgtcg gctttaacag tcacattggc agctccggtg
     5461 agcgcgcacg ggttgctgta accggcaatt cttcgcggat aagcagcgct ggagatagca
     5521 gtcggattgc gaataccgga atgcgagtac gcgtttgtac attgggtaaa cggtgtcatg
     5581 tcgccagtaa tggtgacctg gtgcaaatcg ccagctttgg cgcaaacgcc agaattgcca
     5641 acagcggcga caatgtacac atcattgcca gcggtgaaaa ttcaacggta gtgagcacgg
     5701 gcgtggtcga ctcaattatt ctcgggccag ggggcagcgc cgcactggca tatcatgacg
     5761 gcgaaagagt tcgcttcgcc gtcgccattg aaggtgaaaa caatattcga gccggagtcc
     5821 gctatcgtct taatgaacag catcagtttg ttgagtgctg atagctttac ctttaatcaa
     5881 acgaaagacg ccgacgagca cgggatgcag gctttacgac cgcccgagcc atcgcctcac
     5941 caatttctgc acatgccgta agtccctgaa cgaaaggctg gtcgtgcatc aaccagggca
     6001 acgcgccgaa cgctacgcgc ccacgaatat cttcgggttg ctgtaacgta taatcttcac
     6061 caacatcagg gatttcatcc cctgttttct gtaattgttc gcgtagtcca gggaaaggaa
     6121 tatctttcac tttaagcgga cgctgcccgc gggcatcaat aaaaacgtca aacgagtagc
     6181 tgttgtcttc cgttttcagg acggtgcgcg actcgttaat ttccattttg tagtcttcac
     6241 cgagagcgag aatatgaatg attcccgctt cacgtaaggc aagtaggcga cgaatagact
     6301 ctgaagggat tgccgcataa ttatcgatga aaacccgggc aaggccttta ctgaaccgtt
     6361 tatggtcgtg ttcattcaga tgtggaacaa tttcctgtac ggcttcatgc aggcgcagaa
     6421 tgacataacg ccagggaaca gtatgttttt ctcgtttatt gcgttcgact tcctggagat
     6481 ttttttctgc ccagtcaaat tggtcgcgtt gtttgcgctc ggcaaaccag gcttgagcaa
     6541 aggaatcgac attcaggctc tctaaggcta tgcgttgact ccagtctgga tcagcaaact
     6601 tgatttcctc tactatcaat ctaaataccc gatccaaaag gccatattcg cctttttgaa
     6661 tctcagcatt taatgcctga tcagtgacga tgtgtaaggg ctcgtaggga atagggcaat
     6721 agaaatcggc ttcgggtaaa atacccgtgc gcgacattaa cgtaatattt agcttttcac
     6781 ttgcgttatc gcggtgaaaa acgacgtgtt gtttatcatc ttcaatgaac gaaccatgct
     6841 gaatagccac tgccattgcc gcatccagtc cgctcaagga tgttcccata atacccacgt
     6901 tacacgcatc gacctttgct tccatcaagc ctgaccacgg gcttggaaaa tacgttcggg
     6961 ttgcttcttc ttcatcaggc cagacgtgac ccgtggcgat caccgctaaa tcaaacgtct
     7021 cgctgggtaa atcctgattt gtagcgagca tgacgccagc atttgtaatt tgcagatcgg
     7081 taacctggca tgattcataa acagccactg caaatttttg ctttcgtgcc tggtctacta
     7141 atcgtaaaaa ttgatcgcgg aaatattcgc ccagcagaat tcgcggtaaa aactgacgat
     7201 cgtgcaaggt ttctttttta acgccataac gctggagatg actggcttct tgcttttgta
     7261 gccattcgag atacgtacaa taaatcggcg gtatttcaat actggcaata tttgccagca
     7321 tcatttttga gttttcctca tcactgtatg gcatcccgac accggcctcg tcagcctgct
     7381 cgaagataga aatagaaagt agagtttgtt gctgtagaag cgagaataag gtgtagatcc
     7441 ccgtaggccc ggcacccaca attgcaattt ttttcattgt tacttacctg tacgcattct
     7501 ttcgaattta gtgttatcag ataagtttgg ttgagatttt tagaatttga gaaatttcag
     7561 acagggatga aaagggcgct aaccgccctt gattaaattc tgtcaggcga gttttgcctg
     7621 attgatttga ctcagtggct cattgacatc gaaaactttg gcgacaactt cctcaacgcc
     7681 gagttttttc agttgggcag tgtgtttttc cagataggca agggcgcttt tttcatcagt
     7741 gaaaaggtag atcccaccgg cttcgtggtt cttttcactt tctgtccata ccttccacag
     7801 aaaaccaggt tcctgattaa tcgactctgc aagtggttta agctgctcag ccattgcgtc
     7861 gccaaatggg ccattaaaag caaaatgaag ttgtaacaaa gttgccatag cgattctcct
     7921 tctgataacg catacgaaga cagtgtaaat cttttaacgt attggataca gagaagaagc
     7981 agagggaaaa aggagaagac cagattaaca aaatctggtg cgtccgagtg gactcgaacc
     8041 accgaccccc accatgtcaa ggtggtgctc taaccaactg agctacggac gcaggatggt
     8101 gcgttcaatt ggactcgaac caacgacccc caccatgtca aggtggtgct ctaaccaact
     8161 gagctatgaa cgcaacgttg taggtgacaa cggggacgaa tattagcggc agagtgggaa
     8221 ggtggcaaga ggcaaaacgt aattttctgc gctatttcga ccgtttgcag agcttttaag
     8281 caaattggct atattttgtt gatttgcaag ggcgattttt attcaggatc gcatttacat
     8341 ctgatacaac ccaataattc gtgtatcaga gtttaaacag agttgtttgc caccatctca
     8401 aatctgtcgt ggataaatcc tccgtagcct ttacacttag gtagcaataa aggcacacag
     8461 atgcagcaaa ggaataaatc atgggatctg atgcgaaaaa cttgatgagc gacgggaatg
     8521 tgcaaattgt taagaccggc gaggtcattg gcgcgacgca acttactgaa ggcgagttga
     8581 ttgttgaagc tggcggaaga gccgaaaata ccgtggtcac gggggctggc tggttgaaag
     8641 tggcaaccgg tgggatcgcc aaatgcacac agtacggtaa caatggcacg ctatcggtca
     8701 gcgacggtgc cattgccaca gatattgttc agtccgaggg aggcgcaatt agtctctcta
     8761 cgctcgctac ggttaatggc cgccatcccg aaggtgaatt cagcgttgat cagggttatg
     8821 cctgcggttt gttgctggaa aatggcggta acctgcgtgt actggaagga catcgcgcgg
     8881 aaaaaatcat tctcgatcaa gagggcggcc tgttggttaa tgggacaacc tcagcggtcg
     8941 tggtagatga aggtggtgaa ttgttggtgt atccaggtgg ggaagccagc aattgtgaga
     9001 ttaatcaggg cggcgttttt atgctggcgg ggaaagccaa tgatacgttg cttgctgatg
     9061 gcaccatgaa taatctcggt ggtgaagact ctgacactat tgttgagaat ggagccatct
     9121 atcgtctggg gacggatggt cttcagctct acagttccgg taagacgcaa aacctgtccg
     9181 ttaatgtggg tggtcgggct gaagtgcatg ccggtacgct ggaaaatgcg gtaatacaag
     9241 gtggaacagt gatcctgttg tcacccacca gcgcggacga aaattttgtc gtagaggaag
     9301 atcgcgcacc ggttgaactg accgggagtg ttgcattact ggacggcgct tcaatgatta
     9361 ttggctatgg cgcagatctg caacaatcaa cgcttactgt acagcagggc ggtgtattga
     9421 ttctcgacgg cagtacggta aaaggtgaca gtgtcacttt cagtattggt aacatcaatc
     9481 tgaatggcgg aaaactgtgg ctgatcactg gtgcggcaac gcatgtgcag ctgaaagtga
     9541 aacgcctgcg cggagaggga gcgatttgcc tgcaaaccag tgcgaaagaa atttcacctg
     9601 acttcatcaa tgtgaaaggg gaagttaacg gggatatacg cgttcagata acagatgcca
     9661 gtcggcaaac tctgtgtaac gcactgaaac tacagccaga cgaagacggg attggcgcaa
     9721 cgctccagcc tgcgtaaaaa cacaggcgca ccccgtgaag gttgcgcctt gtctttatta
     9781 gcgggatgct cgttgcagaa tgatggctga cggcagacgt tgcaggtacc gcatacgcag
     9841 catcatcata atggctgccg acgtcaggcc aataataaag cctatccaga agcctgctgg
     9901 ccccataggt tcaacgacca gatcggtcag tgccagaata tagccgcttg gcaagcccag
     9961 cacccagtaa gccgtaaagg tgatatagaa aatggaacgc gtatctttat aaccacgcaa
    10021 aatcccactg ccaatcacct ggattgagtc agaaatctga tataccgccg ccagcaacat
    10081 caaatgcgcc gccagcgtta caacctcggg attgtcgttg tacaacaggg cgatttgctc
    10141 ccgcagtgaa accgtgaata tggccgtcag ggttgccata cagacaccca ccataagccc
    10201 ggtccgcgca gcggtttgcg catccagcgt tgagccctga cccagacgat aacctacgcg
    10261 gatagttacc gctgccgcca gcgacattgg aagcacgaac attagtgaac taaagttcag
    10321 ggcaatctgg tgtcctgcga catcaacaat accgagcgga gacactaaca gagccacgac
    10381 ggcaaacagt gtcacttcaa agaatagtgc cagcgcaatc ggcaaaccga gttgaatcag
    10441 tcgtttcata accgcaggat cgggtttcgc ggtgcctttt tcgttacgaa tatcgcgcat
    10501 cgagcgggcg cgtttaatgt aagaaaccat ggcaaggaac atgacccaat acaccgccgc
    10561 agtagccacg ccacaaccaa cgccaccgag ctcaggcata ccgaaatgac cataaataaa
    10621 gatatagttc accggaatgt tcaccagcag gccgataaag cccattacca tacccggctt
    10681 ggtttttgcc agaccttcac attggttacg ggcaacctgg aagaaaagat agcccggcgc
    10741 gccccacagc aacgcacgca gataacccac ggctttgtcc gccagagccg gatcgatgtt
    10801 ttccatggag cggataatgt aacctgcatt ccacagcacc agcataatga gaacggaaac
    10861 aaaacccgcc agccagaagc cctgtcgcac ctgatgcgca atgcgctcac gtcgaccgga
    10921 accatttaat tgcgcgataa ccggcgttaa tgccagcagc agtccgtgac caaagaggat
    10981 cgccggaagc cagatagaag taccgatagc gaccgccgcc atgtcggtgg cactatagcc
    11041 gcccgccatc acggtatcga caaaacccat cgcagtttgg gcgatttgcg cgagaatcac
    11101 cgggattgct aatgctaata acagacgcgc ttcactgata tacttctgca cgtgaacacc
    11161 ttttatttgt agttatatga gagactaaaa agccgcctga ataggcagca aaaagaagaa
    11221 gcaggggaaa ttcagtctat tgtagcgagg atttattaat tctccagtga aaaaatagcc
    11281 attcttaccc cttcactggt aagggcgttt tccacctgct attgtgctga acagaatgtc
    11341 ttaactgatt tcaggagttg taagtatgtt tacggggatt gtacagggca ccgcaaaact
    11401 ggtgtcgatt gacgagaaac caaattttcg cacacatgtg gtggagttac ccgaccacat
    11461 gctggacggt ctggaaaccg gtgcctccgt ggcgcataac ggttgctgcc tgaccgtgac
    11521 ggaaattaac ggcaaccatg tcagttttga cctgatgaaa gaaacgttgc gcattaccaa
    11581 tcttggcgat ttaaaagtgg gggattgggt aaacgttgag cgtgcggcga aattcagtga
    11641 tgaaattggc ggacacttaa tgtcaggtca tattatgacc accgctgaag tggcgaaaat
    11701 attaacctcg gaaaataatc gccagatctg gtttaaagtc caggatagtc agttgatgaa
    11761 atatatcctg tacaaaggat ttattggcat cgacggtatt agcctgaccg tcggcgaagt
    11821 cacgccaacg cgtttttgcg tccatttaat tccggaaact ctggaacgca cgactcttgg
    11881 gaagaaaaaa cttggcgcac gcgtcaacat tgaaatcgat ccacaaactc aggcagtggt
    11941 agatacggta gaacgtgtgc tggcggcacg agaaaatgcc atgaatcaat caggtacaga
    12001 agcctgatct gaaatcgccc ccggagaggg ggcgatagaa tagcctttag cgagcgactc
    12061 gaaggccgtt ttcaacaccg cgtgagaaca ccacctgcca gagctgaata tcacgggcgc
    12121 ggaaagcacc tgcgcaggca ttcagataat aggtaaacat ccgtttaaag cgttcactgt
    12181 agttatccgc aatttctggc catgcggcga cgaatcgttc ataccacgcc atcaacgtag
    12241 tatcgtaatc agcaccgaag ttatgccagt cttccatcac aaagtggggt tcgctggact
    12301 gagcaatctg gcgtacagag ggcaggcaac cgttcgggaa aatatattta ttaatccagg
    12361 gatcaacatt cagatcggtt tttttcgaac cgatagtatg gagcaggaat atgccttccg
    12421 gtttcaaatt acgatccacc accgtaaaat aggtatcgta atttttcggt ccgacgtgct
    12481 cgaacatccc cacagaaaca atacgatcaa actggtcgtt caggtcacga taatcttgca
    12541 gcaaaatggt gacatccagg ccttcacagc gttcctgagc cattttttgc tgttcggcag
    12601 aaatggtgac gcccaccacg cttacgtcat aattagatgc catgtagtgt gccagtccgc
    12661 cccagccgca gccaatatcc agtacgcgca tcccaggttt taactgcaat ttttcacaaa
    12721 tcattttgag cttcgcctgc tgggcagatt ccagattatc ggcatctttc cagtaagcgc
    12781 aggaatattg catgaaggga tcaagcatgc ggctgaacaa gtcattaccc aaatcgtaat
    12841 gctctttgcc gactatccag gcacgttttt tactctgcag attgaagaga cgggcgccgg
    12901 caatacgcag cgtgtctttg aaatgatggg ggagttggtt ctcgagacct gcgcgtaaga
    12961 ctttgctaaa aaacatatcc agtcggtcac attcccacca gccatccata taactttcgc
    13021 ctaaccccaa agagccttct tgcagaacgc gtttaaaaaa atcggggttt ttcacacgaa
    13081 tatccgccgg ggcagaaccg ttaatggcta taccggcacg gctaagtaat tcgttggcga
    13141 tacggtacca gttgtcatcc ggtacactga cttcttctat acacgatgaa ctcatagttt
    13201 ctccatcact ggtcggtgat cagaacctta aaacagcgta gacgcttttt gggctttgtg
    13261 agaaatctcg cagaaaaaac cgcgagtcta taatccgacg tagaacagag ggaaaggagg
    13321 aacccttgcc gaaatgacca tccatagtga gtcgggaacg atcctgtccc cgtaaaaaca
    13381 taatatttat cgtagcttta acaactcgaa attagtatag gccgctaatt tcaatcatac
    13441 aacaaaattt tgttagcagc ctaagtataa gtatatcgat atagatcagt gtgattcgct
    13501 atgagcgact tcggcattgc catgattctg gcagccaact tcttcacaac gttgcatcat
    13561 gtaacccagc gcgaccagca ctactgttga taacatcacg ctggtggtgg tgagcaatgg
    13621 cgtgctgata ctgatcagcc aggaaactac cagacttgcg aggaagcaca gacccagttg
    13681 cagagtgttc tgcaacgctg cggcgcgacc agttgcgtgt gggaagggac gcagagcctg
    13741 ggcgacaaca atagggtaga tcgcgccatt agcaatcgcc atcacacaga atgggatcag
    13801 gatttcgacc agcgacacat ggctaatgaa gcccgcagcc caggtcgcaa tgacgctgac
    13861 agcaaacagc accagcaacc acggtaataa ctgcttgcct tgccatttct gcagcgcggc
    13921 gcgacagcca taaccaccaa tcagaaacgc gatagtttgc gggacataac ttaaaccaat
    13981 aactgccggg ctgtagccca tttcactaag gatgaacggt gaaccggtca gccatgcaaa
    14041 aaaactggct gaacaggccg cgtatatcag cacattgccg cgataggttt tagaacgtag
    14101 caggtcggta aaggtcagac catcctgact attgttacgg gccttcgtcg tgggtttgag
    14161 ccagaaaata ggcagaatca gcaccacggt aatggcaaaa agggtggcga aaatcgcctg
    14221 ccaggaaaaa tggaccagca gccagcttcc taacagagga gccagggccg gagatagacc
    14281 caccagcggc atgatggtcg caaaaattcg gttaactttc tgtgaaggat aataatccgt
    14341 cactaacgct tgccagataa ccgccgcggc gcagacaccc acagcctgta caaaacgcaa
    14401 taccagcagc atagcggcgt tttctaccca cagcatcccc agactaccta acgcaaaaat
    14461 tgtcaggccg attaataata ccggtttacg accataacga tcggagagcg gcccccacag
    14521 aagctgggct gcggcaaaac cggcaaggaa cagactaagg ctggcactga cagcagacgc
    14581 aggcgtttgc aggtcggcct gtatggcggc gaaagcaggc agatacatat cggttgccag
    14641 aaaaccgagt acgctcaaac ccgccagcca gactaaaaat cttttcccag gttgcatgta
    14701 tattctctaa atctctcaat taaacaccgc cgcaaagtgt agggagtgaa attagtgttg
    14761 tgaaacggta atatttgatg actggtttca aaaattttgc aggcagacta tgtggtcaga
    14821 atattcactc gaagttgttg atgcggtagc gcgtaatggt agttttagcg ctgcggcaca
    14881 ggagctgcat cgcgttcctt ctgcggtcag ctataccgtg cgtcagctgg aagagtggct
    14941 ggcggtgccg ctgtttgaac gacggcaccg tgatgtggag ctgaccgctg ccggagcgtg
    15001 gtttctcaaa gaagggcgct ctgttgtcaa aaaaatgcag atcacccgcc agcaatgtca
    15061 gcagatagcg aacggctggc gcggtcagtt agctatcgca gtggataata ttgtcaggcc
    15121 agaacgtaca cggcagatga tcgttgattt ttatcgccat tttgatgatg tcgaacttct
    15181 tgtctttcag gaagtgttca acggtgtctg ggatgcgctt tccgacgggc gcgtggaact
    15241 ggcgattggc gcaacacgcg cgattccggt aggcggtcgt tatgccttcc gggatatggg
    15301 gatgctaagc tggagttgcg ttgttgccag ccatcacccg ctggcgttga tggatggccc
    15361 gttcagcgat gatacgttgc gcaactggcc gtcgttggtg cgcgaagaca cctcgcgaac
    15421 gctacctaag cgtattacct ggttgctgga taaccaaaaa agagttgtcg tgcctgactg
    15481 ggaatcatcg gcaacctgta tctcggcagg attatgcata gggatggtgc caacacattt
    15541 cgccaaaccg tggcttaatg aggggaagtg ggtagcatta gaactggaaa acccctttcc
    15601 ggattcggca tgttgtctga catggcagca aaatgatatg tcgcctgcgt taacctggtt
    15661 gcttgagtat ctcggcgata gtgagacgct gaataaggag tggctgcggg agccggaaga
    15721 gactcccgca acgggtgatt aacgacgata gtcgcggaac gggccgtcag ccacggagcg
    15781 gcgttcaatc aagcgcggat gcacttcaat ggactgcggt tcttcacgtt tgttgactat
    15841 acgatccaac aacatgttga acgctgtttc acccaacgaa tcttttggct gatggattgt
    15901 tgtcagcgcc ggcgtaaaat agcgggcgtt gcgcacgtta tcataaccga tcagcgaaac
    15961 atcctgcggg acgcgcagcc ccatttcatc agcagcacaa agtgcgccca ttgccatgat
    16021 atcgccacca cagaagacgg cagtagggcg atgcggctgc gacaggattt gctgcatggc
    16081 gcgataaccg gattcaggtt caaagtcacc ctgcacaatc cagctttccg gcaccttgat
    16141 cattgcttct tccatcgcct tcataaaacc ggcaaggcgg cctgcgccag tgttacgttc
    16201 cagcgggccg gggataacgc cgatttcgcg gtgaccgcgt tcaatcagat aacgcccggc
    16261 catgtagccg ccttcgaacg cgttatcaat gaccgcatcg gtgaagtcag cttttgcttc
    16321 accccagtcc atcaccacca ttgggatatg gcgatactct tccagcatcg ccagcaacgg
    16381 ctctgggtac tcagaacaca tcaccagcag accatcgacg cgtttttgcg ccatcatcga
    16441 cagataagcc cgctgtttct caagattgtt ccacgcatta cccagaatca gggtgtaacc
    16501 tttctggaag caattttttt caactgcttc aatgatctcg gcaaaatagg ccgcttcgct
    16561 gctggtcgct agcaaaccga tagacttggt gtggttaacc ttcaggctac gcgccaccgc
    16621 gctaggggag tagtgtaatt ctttgatcgc tgcccacacg gcgttgcgcg tttcttcagc
    16681 gacgaaacgt gttttgttga tcacgtgtga cacagttgta gtggaaacgt ttgctcgttt
    16741 cgctacatct tttattgttg ccattccatt tcactccaga ccctaacttc agctcctgca
    16801 aaatcgcaag gtaaacgttt gcctttacac accttacgca caacaaaagt gttgcggtac
    16861 gccggaaaaa cgacacggag cgtcaggaag gggtcaatgg ccagtacgca aatagtgtaa
    16921 gcgtggaatt ttggccgatc ttgacgaaaa ggggaagtgg taaaaacact tatcattata
    16981 aatgacggga gattttttca tcacagtgtg taaaaatgcg taatatcact ttttgtgtgg
    17041 taataaaaaa ggagaaaact tgatgagcac cgaccttaaa ttttcactgg taacaacgat
    17101 tatcgtcctc ggtttgatcg tggccgtggg tttgactgcc gcgctgcact gatttcctct
    17161 cgagggagcg cgttctccct cgcatctcct tcctgtttgt tagcaaaatg gcaaaagtgt
    17221 tttctctttt tgttattttc atttttttgt gattgctgtc atgctttcgc cactttaatg
    17281 aatatcgcgt aatgacgcga agaggtggag ataaggcatg aaaattaact atccgttgct
    17341 ggcgctggcg attggcgcgt ttggtatcgg gacaacggag ttctcgccaa tgggcttgtt
    17401 gcccgtcatt gcgcgcggtg tggatgtctc gattcccgct gccggaatgt taatcagtgc
    17461 ctatgcagtt ggcgtaatgg ttggcgcgcc gctgatgacg cttctacttt ctcatcgtgc
    17521 ccgccgcagt gcgttgattt tcctgatggc aattttcacg ctcggcaacg tactttccgc
    17581 catcgcgccg gattatatga ccctgatgct ttcacgcatt ttgaccagcc tgaatcacgg
    17641 agcatttttt ggtttgggtt cagtcgtggc cgcaagcgtg gtgccaaaac ataaacaggc
    17701 cagcgcagtt gccactatgt ttatggggtt aaccctggca aatatcggtg gcgtgccggc
    17761 ggcgacctgg ttgggtgaaa ccatcggctg gcggatgtca tttctggcaa cggcggggct
    17821 gggagtgatt tcaatggtaa gtctgttctt ctcattacct aaaggtggtg caggggcacg
    17881 acctgaagtg aaaaaagagc tggcggtatt aatgcgtccg caggtgctgt ctgcattgct
    17941 gacgacggta ctgggagctg gtgcaatgtt tactctctac acctatatct ctccggtact
    18001 gcaaagtatt acccacgcaa caccggtgtt cgtcacggca atgctggtgc tgattggtgt
    18061 cggattctct atcggtaact atctcggcgg caaactggca gatcgttcag ttaacggcac
    18121 gttgaaaggc tttttgttgc tgctgatggt gattatgctg gcaatcccgt tcctggcccg
    18181 caataagttc ggcgcagcta ttagcatggc ggtgtggggc gctgcaacct ttgcggtcgt
    18241 accgccgtta cagatgcgcg tgatgcgtgt cgccagtgaa gcgccaggtc tgtcttcatc
    18301 agtcaatatt ggtgccttta atcttggaaa tgcgctggga gcagctgctg gtggtgcggt
    18361 aatttccgct gggctgggat acagctttgt gccggtgatg ggggcgattg tcgcgggact
    18421 ggcattattg ctggtgttta tgtcagccag aaaacaacct gaaacagttt gcgttgctaa
    18481 cagctaatta aacaaaaacg cagaaggata accttctgcg ttacagttgc tgacaacgtg
    18541 cgatttatct tgccgaatgc gctgttaacg cctcatccgc gttggcatgg taagcgaagc
    18601 gcatcaggca atactgcatt tgccatcagt tattatgcag cgagattttt cgctacgaat
    18661 tcccagttca ccagcgccca gaagtgctca agatagccag gacgtgcatt gcgatagtcg
    18721 atgtaatatg cgtgttccca gacatcaacg gttagcagcg gagtcgcatc ggtggtcagc
    18781 ggagtacccg cgttagaggt tgaaacgata gccagtttgc catcgctgtt tttcaccagc
    18841 caggtccagc cagaaccaaa gtttttgatc gctgcatcag taaactgagc tttgaaatcg
    18901 gcaaagctgc caaaagatgc ggcgatagct tcagcgactt ttccagtcgg ttcgccaccg
    18961 gcgttcggtg ccaggcagtt ccagtagaaa gtatggttcc agacctgagc tgcgttgttg
    19021 aatacgccac cttcagagct gcgaataatc tcttccagtg atttaccttc aaacgcggta
    19081 cctttaatca ggttgttcag gttagtgaca taagtctgat gatgcttgcc gtagtgatac
    19141 tcgatagttt ccgcagaaat gtgcggtgcc agagcatctt tagcatatgg tagtgcaggt
    19201 aattcgaatg acattgctac tctcctttat tattaatttg catacgtaca atagccttat
    19261 tgtgcgtatg gatagggtag caaattaaaa ggtgagacaa aagatgaact taccctgttg
    19321 ccgtaacaac agggtaaagt tttagcgaag tgtttttggg gtcattaccc ggcgagcgcc
    19381 aacatagtgg cgctgccagt agtcttcact gagagaagtg atttgaattt cctgacctgt
    19441 gcgcggtgac tgaataaatt tgccgttgcc gacatacacg ccgacatgat cggctgtacc
    19501 gcgtccctga gtacggaaaa agaccaggtc gccgtttttc agttcactac gttcgattgg
    19561 cgctgcatcg cgcaggtgat acatttcatt cgccgtacgc ggaatacgaa ttttcaccaa
    19621 atctttataa gcgtaataaa ccaggccgct gcaatcaaaa ccggtacgcg gtgagctgcc
    19681 accccaacga tatggcttac caatttgctg catcagttta ttcattgcca cttttgtggc
    19741 tttttgcacc ttcgctttgt gtgcgtcggc aagggttcct gacgctgcat ttttgacttt
    19801 cacacaatgc gatttatagc ccttacgttt ggtacacttt tcggtaaagc tggctgaggc
    19861 cgttttattg acggtatgtt tgctgcgagt tttggcgttt ttagaagcgg taatggaaga
    19921 ttttttaacg gaagaagcgg ttttgctttt ggtcgtactt ttactggcgg cttttttcgc
    19981 tgttttctgg gtctttttgg tggttgccgt gcttttcttt ttatctgctt ttttggtgat
    20041 atgagtggta gcagaactct ccctggcttg ctttgaagca tgggccatag gcgttaaagg
    20101 cagggtggta aaaagtaaag cacagagcgt gatcgaaata cggtttatcc gcgccactga
    20161 gcgtccctct gttaaggcag gtctatcatg cctgcgtctg atttacaaaa cttgatgatt
    20221 ctaatgcata gaagaagaga aagttaatag cttttattgt taaaaaatac gtttcgttgg
    20281 cgagcgcaaa aaaatgcagc agtgaaagct aaaaatggtc ataaatacgc cagtttgccg
    20341 tgcatcaggg aagaaacgaa tatttgaaat gcaacaaata gagcaaaggg ataaaatagc
    20401 aaaagcgtga tgaaaatgtt attttccgat gccgcactga cccgctacaa tgtcagatta
    20461 ctgccgtaaa gaagtttgag gaagcaagac aatgagcacc actatcgaaa aaatccaacg
    20521 ccagattgct gaaaacccga tcctgctgta catgaaaggt tcaccgaaac tgccgagctg
    20581 cggtttctct gcccaggcag tccaggcgct tgccgcatgt ggcgaacgtt ttgcctatgt
    20641 tgatattctg cagaatccgg acattcgtgc ggagctgccg aaatatgcta actggccgac
    20701 cttcccgcaa ctgtgggttg acggcgagct ggtaggcggt tgtgatatcg tgatcgaaat
    20761 gtatcagcgt ggcgaactgc agcagctgat caaagaaact gccgctaaat acaagtctga
    20821 agagccggac gcggaataat ccgtcctgca atactgacgg atgcgaacgg gcatccgtca
    20881 gtcctttctc tatccccaat ccagcccttg tggcgaactt gaaaatcccg cttcacgcag
    20941 cagcgtaaac atcggcgttt gccagaccgg tagatcattc acttctgtta gcgtaaagcg
    21001 cagccgtggt tcgcgacgca gtgcggtagt cagcgcgtgg aaaacctctg gggcgagtaa
    21061 ttcctctttt tcctgccaga ccagcatttt tttgccacct tgcgccagat agagtaacaa
    21121 tttgccgcca gaaacgacca ccagcgcacc cgcccgacgc gttggtacca gcgttgccgg
    21181 atgtgcaggc cagggcagaa gatttcccca cacattagcc ggatcgttgg cggagagcgc
    21241 cactggtgta tagtggcgcg tttgcgcggc ttgtgtcgcc aggtcgcgca agcggtcaat
    21301 agtcagacgt tcagcgaatt gcgcgccacc cagaccttct acaaaacgac ctcgcataat
    21361 tcgcccggaa tcttccatac ttcgacaaag cgtttgcatc gatggaaacc cgccagggat
    21421 attttcggct atcaccgcct gacgactgat gatgccgtag cggtcgagca tattttccgc
    21481 cagcgccagc atcctttcgg tatcgtttag tggctccacc tgcaataacg accagcgtcc
    21541 agccagattt ggtgtgttgt aagatacccg cggcgagacg ggttgtgcat agacaggacg
    21601 cccacgacga gcccggtgac tgcggcgagt tgaggtgcgt gcgttggaac tgctgcgggt
    21661 gagggcgcgt aacggtgccc aaatatcgct ggtgatgacg ccttgccaga ccagcgccca
    21721 taacgcctct tgcagtgccg agggatcaac cgattcgccg attttgtcgc gtatccgttg
    21781 gctgatttgt tgtgcaaacc aggctcctcc gtcagccaga acagcgacta tcgcttgttg
    21841 cagcgccgaa cgattcgcct gatccgcttc ggcgggagtg aacgattctg cagcatattc
    21901 ctgtagatgc agtgccacca ggccgtcatc ttcacccagt tttttttgcc ccgaccagat
    21961 aaccgcaccg gttgccagta attcatcgag cacttctggc gaatagtcgt gtacgcgggc
    22021 aggcaagatc tggctttccc agagtgaggc gggtaagccg actccggcaa gctgttcgat
    22081 cacccgcatc acgccatcca ccccctcata aacgcctggc gatgttgagg caaagagcgc
    22141 cgggctacca tcggtggcgg gtaatacgcc ctgacgttcc agcagcaatc gcgcataggt
    22201 cgtggctgca acgggacgcg tcgcttctct ggcggcttgc agcgagcgca aacgcagacg
    22261 acgaaatact tcatcgctca cccagatgtc ttgttgcaga ttcatcacca gaccctgttc
    22321 gcgcagttgc tgaagctgct cttcgacaat ggcaataccg agactaaact catgagccag
    22381 ttgttcagcc gtgaccagag catgagcccg gagatagcgt agaaagaggt cgcgaagcgg
    22441 gtaactgact ctatgcaaat aaatctctgg cagtgactct ggtaatcgta cgccgagggc
    22501 atcgcgcagc ctggcggcat catccataca ggcaagacgc tcctgtccac taatcatcgt
    22561 cgggaagatg cgttttactg taagaagatt ttccagatac gaggcaatct cttcactgct
    22621 gcctgtatgc cgttgcgcca ggtcttcaac ggtcattggc cccagttcgc gcagcaggtc
    22681 gaacaatcct tcttcacctt tcgctcttct gccaggagcc agtcgttgca actcttcttc
    22741 cacctggcga atgacctgcg ggtcgagtaa ttcccccgga tcgacctgtc cgagtagatt
    22801 gcgcagtaac tcgctgtcca gcgacagtac ggatgcccgg cgttctgcca gcggggcgtc
    22861 gctctggtac ataaattccg cgacatagcc gaacaataaa cttgtggcga aaggcgaggg
    22921 cgtagtagtc gttacatcgg atatttgaat ttcgcctccg ttcaggcgac gcatcaaacg
    22981 ttccagtgcg ggaagatcat aaacatcttg cagacattcg cgtagggttt cgagaatggc
    23041 cggaaaatcc ggatatccct gagcgatttc cagcaactga ctggcgcgca gccgttgttg
    23101 ccaaagcggg gtgcgatggc ccggcgtgcg gcccggcatt aataatgccc gcgcggcgca
    23161 ttcgcgaaaa cgggcggcga aaagtgccga actgcctacc gcctcgcgga caatttgcag
    23221 caacttttct ggttcaaaca aaaaaatcgc ggcgtcgggc aatttaccat cagtgtcagg
    23281 aatgcgcgca acaatgccgt catcactggc gaccacagac gcgtcagcgc cccaaagcgc
    23341 atgtattcgc ccggcaatcg ccagcgccca gggttcatgc acccgtcttc catagggaga
    23401 gtgcaaaata atacgccaat cgccaatttc atcacggcac cgttcgagga ccagatgacg
    23461 actccccgga acaatacccg tcgcgttgcg ctgctcgtca atcaacccct gaatattggc
    23521 gatcgtattt tcttcagcca gccacggggg aatagtcccg gaaaagaacg ctccatccgc
    23581 cagcaaatga agaaaatcgc cgatcatctc gcctaattca gccggacgtc cgttaccttc
    23641 accacgccag aaggggagcc gggcagaacg acccggagca ggagtcacaa tcacctgatc
    23701 gcgggtgatt tgctggatcc gccatgaggt tgcgccgaga gtgataatat cgttcacccg
    23761 cgactcatat accatctcct catccagttc acccacccgt cgcgaaccgg cctgttcttc
    23821 cccttcgggt aataacacgc tatacatgcc acgatccgga atggtgccgc cgctggtaac
    23881 cgccaacagc tgagcgccag gtcgggcggt caatatcccg gtctccctgt tccagaccag
    23941 tttggggcga aaagcagaaa aatcgccaga gggatagcgt ccggaaagca tatccagcgt
    24001 ggcgtcaaaa acacgtcttg gcagatcttt ccacggtgcg gcacggcgta cgcgggagta
    24061 ccattcgtct acctgtaatg catccatcgc cgcggcggca acggtgtgct gcgcaaggac
    24121 gtcgagtgga ttatgcggag gtgtcaggtt ttccagcctg cctgcgaaca tacactctac
    24181 aatgactgcg gaatcgacta agtcacgcct ggttcggggg aaaaaaagcc ctttggagat
    24241 acctcctatc tggtgtccgg cacgtccaat gcgttgtaac ccactggcaa cagaaagcgg
    24301 cgttgccacc tgaatcacca gctccacagc gcccatatca atccccagtt cgagactgga
    24361 agttgcgacc acgcagcgta actcacccga tttcagcgcc tgctcggtga ttgctcgttg
    24421 ttctttagag acggagccgt ggtgtgagcg ggcaataaaa acgtcgctac tttgtacacg
    24481 gttagaggtt gcgccagagg tcgactcgaa atgggccgca tcagcggcga tagacgggga
    24541 acgctgtaag cgtgcggcgt aaagctcatt taatcgtgcc gtcagttttt ccgccagccc
    24601 ccgcgaatta gtaaagacaa tggtcgagcg atggcgcaac acttcatcaa ggatacccgt
    24661 ttcaatatat ggccagatgg agccttcccg gccggcatgg ctgtcttcgc cagtgccgct
    24721 ggcgaccgat gagacatcat ccatattggc gactggtacg acaatccgta tctgcggatg
    24781 acgcattgcg ggcgggttga ctaccgtaac cgggcgatcg ccaccaagaa atgctgccac
    24841 atcgctggct gagcgcacag tggcagaaag gccaattcgc tgtgctgagg tgtggagcag
    24901 cgcatcgagc cgctccagac ttaacgccag atgcgcacca cgtttacttc ccgctaccgc
    24961 gtggacttca tcaataatta ccgtttcgac gccgcgtagc gtttcgcgcg cgcgggaggt
    25021 cagcatcaga tagagtgatt cgggtgtggt gatcagaata tccggcggat tacgggtgag
    25081 tttgctgcgt tcctgtgcag gcgtatcgcc agtacggatc cccacgcgaa gattgacttc
    25141 cgtttcgccg cgccgccgcc gttcatcggc aatacccttc aacgggatct gcaagttgcg
    25201 ctgaacgtcg gtgcccaggg cttttatcgg tgaaatatag aggatgcgtg aggttttacg
    25261 tttatgcgcc tcgcgggtat cttcgccgcc ttcgcggaag agtcgatcga gggcgtagag
    25321 aaatgctgcc agcgttttcc cggagccagt cggtgcaatc accagcgcat gttcgcctcg
    25381 cgccgccaca tgccaggttt gcgactggac agcggtcggc tgtttaaagg cgcgaagaaa
    25441 ccagtcgcgg gtcgccggtg aaaacacgtc cggcaggggc gatgaagggt ttggattatc
    25501 tgccattaac gctattcccc gtgaatatca tgcatgatgc gtggaatagc ctcactatag
    25561 atgcctgaat cattgcatgt catcaggcat cgactcgatt acacctcttc ggcggcaggt
    25621 agcggccagc ctcccagacg tttccagcgg ttgacgattt cacaaaacag caccgcagtg
    25681 cgttcggtgt cgtacagcgc ggagtgcgcc tgggtgctgt cgaagtccat gccagcggtc
    25741 tggcaagcct ttgacaatac ggtttgtccg agtgccagcc cggccagtgc agcagtgtca
    25801 aaagtggcga aagggtggaa cgggttacgt ttcagtgagg cgcgttctgc ggcggccatc
    25861 ataaagctgt gatcgaaatt ggcgttgtgc gccaccataa tggcgcggtt acagccgctc
    25921 gctttaatac ctttacgtac aactttaaaa atttcgtgca gcgcctcgta ttcgctgacc
    25981 gcgccgcgat cgggatcgtt cgggtcaatg ccgttgaaag caagggcttc tggttgcaaa
    26041 tttgcgccga caaatggttc gacgtggaaa tgtaatgtgg tgtccggcat cagccagcct
    26101 tgttcatcca ttttcagggt gatggcggca atctcaagca gcgcatcggt tttggcgtta
    26161 aatccagctg tttcaacatc gatcacaaca ggataaaagc cacgaaaacg gtcgcacaga
    26221 ccggtaagtt gagcgttatc ggacatctga ttctcttaat acggataaat tgcagcgcgc
    26281 attatgacaa atattacttg tcgatgcagt aaagatgcgg gcgcgatgag ttcgcgcccg
    26341 gcaggagatt agttgcccag accgcgaccg gcgtctttct cttcgattaa ctcaattttg
    26401 taaccgtccg gatcttccac aaacgcgata accgtagtac cgccttttac cggacccgct
    26461 tcacgggtca cgttaccccg ttttgacgga ttttttcgca cgcttcagcg gcattatcta
    26521 cgctaagcgc gatgtgacca taagcagtgc cgagttcgta tttatccacg ccccagttgt
    26581 aggtcagttc aatcaccgct tcttcggttt ccgggccgta gccaacaaac gccagtgagt
    26641 atttgtattc cgggttttcg ctggtacgca gcagtttcat gcccagcact ttggtataaa
    26701 aatcgatgga gcgttgcaga tcgccaacgc gcagcatggt atgaagaaga cgcatctttt
    26761 tatcctcaaa atggctaaca gattcaaaat gttgttttag tatagcggcg gctttacgcc
    26821 gctcgcaatg ttggattaca acgtcgggta atcagtgtag ccttccgcac cgccaccata
    26881 gaaactttca gcacgctgtg ggttaagctc agctttgcgc tgcaagcggg cgaccagatc
    26941 cgggttcgca atccagtcac gaccaaatgc caccgcatca attaaccctt tgccgatcag
    27001 cgtttcagct ttttctactg tgtatgcacc tgcgccgata atcggaccgt ggaaacgggc
    27061 gcgtactttt tcgcggaacg catcagtata cggttcaccc cccgcccaat ctggttctga
    27121 catatgcaga taagcaatgc cgcgtttacc cagttgttca atcagataca gtgcatcggc
    27181 ttcttcattc gggccgttat ccgtgttctg gaaagtaccg attggcgaaa cgcgaatgcc
    27241 aatgcgatcg gcaccccatt cttcaatccc ggcatcgacc acttccagta ccaaacgtgc
    27301 gcgattttcc acgctgccgc cgtactgatc ggtacgatgg tttgaagaag gagaaaggaa
    27361 ctgatgcagc aaataaccat gagcagagtg gagctctacc agatcaaaac cggcttcacg
    27421 cgcgttagca atagcctgac ggaaatcatt gacgatactt ggaatctctt ccagttcaag
    27481 cgcacgcggc atggatgttt caacacggat cgcctgacca ttttcatcgc gcagagaagt
    27541 acgtgttccc gcgctaagtg ctgaaggcgc tactggtgcc tggccgccag gttgcaggct
    27601 ggcgtgagaa atgcgtccgg tgtgccacag ctgtacggcc atatgcccat tttcagcatg
    27661 aacgccagcg gtaatttttt tccatgcggc aatttgttcc ggactatgga tgccaggtgc
    27721 accagcatac ccttttgcct gggcagaaat ttgcgtggct tcgctaataa tcaaaccggc
    27781 actggcacgt tggcgatagt attccgccat caacggggta gggatgtcac ccggttcaat
    27841 actgcgcaga cgcgtcagcg gtgccataaa aatacggttt gccgcagtta ttgcgcccac
    27901 tttcagtggg gaatacagtt tttcagatga cataaaggct cccggtcgtc tggtgataaa
    27961 aaatgcctgc taaacggcag gcgtcgcaat aatggttttt acatgtgcca gcgcgttttc
    28021 cagtggctcg taattgcgcg aaattttggc ctgcagattc gcacccagcc atagtgcgta
    28081 aagcacttgt gcctgttgca gcggttcgcc acaaaaggtt aaacaatggc tatcacggcc
    28141 attttccaga gcctgcgaga gcagggcgat cacgccgcga gcgcctttat ccatcgcgct
    28201 acgcatatct tctgacagat cgcacacttc ggcagagagt tttactgtca ggcaaccact
    28261 gatggttcca tgttggcaaa actggttcag tgtttgctgg taataagcca gtatgcggtc
    28321 gcggtagtta ccttcgccgg attgcagcaa ctcagtcagt cgttggtgat atgtggcgta
    28381 atggcgctca agcatggcaa cgccaaacgc ttctttagag cgaaagtagt gatagaagga
    28441 ccctttcggc acttcagcgg tttttagtaa ttcgcttagc cccatcccgg tgaatccacg
    28501 ttgcaggcaa agttgctcgc ccgtcgccag gagatgttcg cgagtatcat gttcggtgtg
    28561 tttgttcatg cgttggagtg tagtagacca gtcggtctag tgcaagcaaa gttgctactt
    28621 tgggcattaa cagcttcaat aatggtaata atcttatttg aggagggcga agtggcggag
    28681 caattagagt tctttcccgt ccagagcccg tgccggggaa tttgccagtc tgacgaacgc
    28741 gggttttgtc gcggctgttt tcgaagccgg gatgagcgtt ttaactggaa taaaatgagc
    28801 gatggcgaaa aacaagaggt gctgcgcctt tgtcggcagc gattaatgcg caaattacgt
    28861 gcaaataagc cagcgtcatc agacgaaccg gagcaaccat cactcttttg agcctaatct
    28921 tgcgtatact cctctcatat ctgataagag gaagcgatta tggttcagcg tattactatt
    28981 gcgccgcaag gcccggagtt ttcccgtttt gtgatgggct actggcgatt gatggactgg
    29041 aacatgtccg cccgccagct ggtcagtttt attgaagagc atctggatct cggcgtgacc
    29101 accgtggacc atgctgatat ttatggtggc tatcagtgcg aagcggcgtt tggcgaggcg
    29161 ctgaaactgg cacctcacct gcgtgagcgg atggaaatcg tcagtaaatg cggtatcgcg
    29221 acgaccgcgc gtgaagaaaa cgtcattggt cattacatca ctgaccgcga tcacatcatt
    29281 aagagcgccg aacagtcgct aattaatctt gcgaccgatc atctggatct gctgttaatc
    29341 caccgcccgg acccgttaat ggatgccgat gaagtggcgg acgcgttcaa acatctgcat
    29401 cagagcggca aagtgcgtca ttttggcgta tccaacttca cgcctgcgca atttgccctg
    29461 ttgcaatcac gtctgccgtt tacccttgcc actaatcagg tggaaatatc cccggtacat
    29521 cagccgttac tgctggatgg cacgctcgac caactgcaac aactgcgtgt tcgtccgatg
    29581 gcgtggtcct gccttggtgg tggtcgtctg tttaatgatg attatttcca gccgctgcgt
    29641 gatgaactgg ctgtggtggc ggaggagtta aacgcgggct cgattgaaca ggtggtttac
    29701 gcctgggtat tacgtttacc gtcgcagccg ctgccaatta tcggctgagg taaaattgag
    29761 cgcgtacggg cagctgtcga agcagaaaca ctgaaaatga cccgtcaaca atggtttcgt
    29821 atccgtaaag cggcactggg gtacgacgta ccgtaagcac ttttaggaat agccgccgtt
    29881 caaaaatgtg tcactggttt acacttattc aggaatgcac aatgaacgga ggtcctatga
    29941 aacgttttag tctggccatt ctggcgctgg ttgttgcgac cggcgcacaa gctgccagtg
    30001 aaaaagtcga gatgaacctc gtcacgtcgc aaggggttgg gcagtcaatt ggtagcgtca
    30061 ccattactga aaccgataaa ggtctggagt tttcgcccga tctgaaagca ttacctcccg
    30121 gtgaacatgg cttccatatt catgccaaag gaagctgcca gccagccacc aaagatggca
    30181 aagccagcgc cgcggaatcc gcaggcgggc atcttgatcc acaaaatacc ggtaaacatg
    30241 aagggccaga aggtgccggg catttaggcg atctgcctgc actggtcgtc aataatgacg
    30301 gcaaagctac cgatgccgtc atcgcgcctc gtctgaaatc actggatgaa atcaaagaca
    30361 aagcgctgat ggtccacgtt ggcggcgata atatgtccga tcaacctaaa ccgctgggcg
    30421 gtggcggtga acgctatgcc tgtggtgtaa ttaagtaatt aagaggccag cgtaccttgc
    30481 ggtggtgctt gctcaagttg cgaaagcgag cagtacagcc gccagacaat tgccgccagc
    30541 tcacgggcgg caggttgatg atggcgggca aggctgtcgc aaatccgctg taattcttca
    30601 agtgtggccg ccagtgattt ttgctgaacg ccacgctcac tcatcactcc ccgcaacagt
    30661 gaaatacagt tatcccgtac tcgcgataac ggatcggaac gcgattccca gtcgcgtaat
    30721 tgccagacaa catgagaaca gttcagcagc actatacccc agcgtaataa ccaacggcga
    30781 gccagcgcat cctggctgtt actcaactga ctgacgtgat gataagtgag cgattcaaat
    30841 tcgctttcac tcagtgttgg atggcggctt agttgatcga caaaatcccg gcgcagcgcg
    30901 cgaatatggc ggcggctttt gcgagcatcc gatcctggac gcagaatggc gaacgctaac
    30961 cacgccaacg cgacgccaac gatttttgcc agattatcgt taagaaaatc agcaaaatta
    31021 tacaccgggg gattagtgac ggcgataaaa gaacccataa aaacaatcag ttgcccccac
    31081 aatgcggcaa atttgggcat ctgcaattta agaagctgca ttgtcgccag cagtggaaag
    31141 agaaacagta aaaattgcca cagatcgcta atctggacca tcagaccaaa tttgaccaca
    31201 aagctgaata gcgaaagtaa caccagcgtg cgcatcagca gtgacaacga cttaaacggt
    31261 gctgcgacgg cggagtagag tacgcagcta atcgctgcca gcgttaatgc attggcaccg
    31321 gcatcccatt gcgaagcaat actccatgcg ccaatcatca tcaacgtaca aaatgtacgc
    31381 agtccgctcc acatagcttc ggcgttatcg gtatgacgag ccaggccgga tgtccgtggg
    31441 agtctggcat gatcatctac atcgctttgc agacgatgta attcctgact actttgcaga
    31501 taaaggcggc aaaaataacg tagtcgctgc cagaaggcga cgtgccgata gtcggcgacg
    31561 ttggtcgggc gtagcggggc gataatacgt gcgacggtgt aaacatctgt ttgcgaactg
    31621 gcgagcgccg tcagcaactg ttcgagaatt tctcgcgtgg cacctggcgg tgagggccag
    31681 ttgagcaaca tacgtcgcag gctggagatg acactggtca tacgtaattg ctggtggagc
    31741 agcgcattaa ggcgcgcgtt ttgctggcga aaacgatagt ggctccagaa agcctggata
    31801 cgcagcaaat tcatggtcag tatctgccca atcacccctt catgtgctgc acgaatggca
    31861 tcggttgttt caggctgcca gagtaaactg gcatgttcca gtaatcgggc gtgcatgttt
    31921 ttcaatgcgg ttaaaagggc tgtagcatcg gaactgctag gcaggatcat catcatcatg
    31981 ccgccgcaca aaataccgac aatcacctcg caaacgcgcg cctgagcgat atcccacagc
    32041 tggctggcct cagtaatatt aaccatcgga aaggcgatga tggcagccgt gtagcccgcc
    32101 agttgaaatg catacgcgac gttattcgtg aagtgcgcac aggcccaggt acaaaagcca
    32161 agccacgccg acatgctcaa tagaaaaaac cacggctcat tgagcgtatg cccggcaaga
    32221 agcagtgccg caatggctcc cagtaaactg ccagcgatgc gtccgaggct tttgctgatg
    32281 acaccgccaa cggtgggaaa gctaaccact gcagccgagg tcatcgccca atagggttca
    32341 tccagattca aataataggc aaccgtcagc gccagacaca tggcaatggt attgcgtaac
    32401 gcataacgcc attgcgccag cgtggccctg aaccagggca aattgcgcaa ggaccatgac
    32461 gatgcgttca ttattgacct acggcaatgc tacaggtggt gcccgacacc agcgtgacat
    32521 cgcctggaac tttatccagc gcaaagcgga cgggaacgcg ttgggcgaga cgaacccagg
    32581 ggacgttagg tttcacatcc ggaataaggc ttgagtcact ttcaacgctc tgatcataaa
    32641 tcgcccgacc gatactggaa acgtgaccct gtaacgtttt gttatcacta tagagggtaa
    32701 tttgtgctgg tgcaccttcg cggatatggc gcaacttggt ttcttcaaaa taaccaatga
    32761 cgtaaaacga gtggctgtcg acaagggcaa acagcggttt cccggtgtca gcgtaatcgc
    32821 cgatgcgggt agtgagatta gtcacccatc cactcaccgg ggcgcgaatt tccgtttgcg
    32881 ccagttgcca ttgtgtctgt ttcagcgtcg cctgtgcggc atcaacactg gcctgcatcg
    32941 ctttaacatt gaggttagcg gtatcgagtt cttcggcaga aataaaattt tgtgagagat
    33001 gacgacgacg attcgcttcg ttattggctt ttgcgaggtc agattgcgct ttcgccagct
    33061 gagcctgtgc attcagctcg gcgatctgaa agggcgtttt atcgatcgtg agcagaagat
    33121 cccccgcgtt aaccagctgg ttgtctttta tattcagctc aacaatacgt cccgacacct
    33181 gtggggtgat agaaacctgt tctgcgcgta ttttcccgtc gcgtgtccac ggagattgca
    33241 tgtaataatt ccacagccac catccggcaa gaaccgcaac taccgctaca atgattgttg
    33301 agaaatactt aattgtttta attgacatag ttaccacgca atcagtattg ccagagcaag
    33361 gcaaacgcaa atcgcaaaca gcgataaatc cattaacaag ggatgccaga tgtcaccggc
    33421 gtagatccag ccacgaagca ggcggtgtac gacaagccag atgacgaatc caaacgcgaa
    33481 ggctttgaaa aacggaggaa agtagacgga cgcaccgaac accagatctt gtaagggcaa
    33541 tcctgttgca ttgagcataa acttcacaaa gtagattcct ttacgaccga atatattgcg
    33601 ttagattaat aaatattctt taactgcgaa aaatttacgc gcaatttctg aaaaatatgt
    33661 ctgacggtaa ccaaatgcag caatacattt gttttagcaa tacaattgct gcacactatt
    33721 ctaaaagccg cataatatct tagcaagcta attataagga gatgaaattg gaatcgccac
    33781 taggttctga tctggcacgg ttggtgcgca tatggcgtgc tctgatagac catcgcctga
    33841 aaccgctgga gttaacacaa acccattggg ttacgttaca caatatccat cagttacctc
    33901 cagaccagtc gcaaattcaa ctggcaaaag cgattggcat cgagcagcca tcactggtcc
    33961 gtactctgga ccaactggaa gaaaaagggt taatttcgcg tcaaacttgt gccagcgatc
    34021 gtcgggctaa acgtattaaa ctgacggaaa aggcagagcc gctgatcagc gaaatggaag
    34081 ctgttattaa caaaacccgc gcggaaattt tacatggcat ctccgcagag gaactggaac
    34141 aactgattac gctcatcgca aaacttgagc ataatatcat tgagttacag gccaaagggt
    34201 gaaatgaagg gggcgtgtgg tcagtaacct gaccacacgc aaacttattt agcgcggaga
    34261 aacggtcacc tgactgccat tgctggccag tacgacacgt tggcccggag agaaacgagt
    34321 gttgccttgt ttctgtacca ccatgatggt attaccatcg tctttacgaa tttccagctc
    34381 gacaccttgc gttttgttca ttgcactctg tacgccctga ccagctacgc cacctgcaac
    34441 agcgcctgct gcagtagcca gagaacgccc ggttccgcca ccaacagtat ttcccaggaa
    34501 accaccaaga acagcaccgc caattgcacc gataacgttg gaatcatcac cgccctgaat
    34561 ctgtaccgga cgtacgttaa cgatggttcc atagctgaca ttctgtactt gtttcgcttc
    34621 agaagcggta taaacatccc ctgacagggt gtcgttatta acacaaccga caagagacag
    34681 acctaccatt gaaacaacca atacgcgttt aatcattgaa aaatctcctg ttcaccatga
    34741 aacgccacgc aagcatccct catggtcaaa gtatatggca tatgtgaatc catggttaca
    34801 tgttctacca aatatgagca aatcataatg aaagtaggat caaccaatta taaacaaaac
    34861 attcatgatg attattgatt tgaagataat gttttactac caacggtaaa aatcggtcat
    34921 tccgcatggt attcacgaaa agtttatgtt tgagtgtgcg caaacgtcag attgaaaaag
    34981 gatgaactat gaaatcgggc cgctttattg gcgttatgtc aggcaccagc cttgatggtg
    35041 ttgatgttgt gttggcgaca attgatgaac accgggtcgc acagctggca agtttgagct
    35101 ggccgatccc ggtgtctctg aaacaggctg tactggatat ttgccagggc cagcagctta
    35161 cactttcgca gtttggacag cttgatactc aactcgggcg actttttgct gatgcggtca
    35221 aggccttgct taaggaacaa aacctgcagg cgagagatat agttgcgatc ggctgtcacg
    35281 gtcaaaccgt ctggcatgaa ccgacgggcg tggcaccaca cactttacag attggcgata
    35341 acaatcaaat tgtggcacgc accggaatta cggttgtcgg tgattttcgc cgtcgcgata
    35401 ttgccttggg aggacaaggc gcaccgctgg tacctgcgtt ccatcatgcc ttgctggctc
    35461 acccaaccga gcgacgaatg gtgctcaata ttggcggcat cgccaatctg tcactgctca
    35521 ttcctgggca gccggttggc ggctacgata ccggtcctgg taacatgctg atggatgcct
    35581 ggatctggcg tcaggccggt aaaccttacg ataaagatgc cgagtgggca cgggcgggta
    35641 aagttattct cccactgctg caaaatatgc tcagcgaccc atatttctcg caacctgcac
    35701 cgaaaagcac cggacgcgaa tactttaact acggctggct ggagcgccat ttacgccatt
    35761 ttccgggtgt tgatccccgt gatgttcagg ccacactggc agaactcacc gccgtgacca
    35821 tttctgaaca agttttgttg agcggtggct gcgaacgatt gatggtatgt ggtggaggta
    35881 gtcgtaatcc gctactcatg gcgcgtctgg cggcattact gccaggcaca gaagtcacca
    35941 ccaccgatgc cgttggcatt agtggcgatg acatggaagc attggctttc gcttggcttg
    36001 cctggcggac gctggcggga ttaccaggaa atctgccttc cgtcactggc gcaagccagg
    36061 agacggttct gggggctatt ttccccgcca actcgtgata tcagaattaa ctgaattttt
    36121 tcttatcgtg tctgccgtta cacttggaag attaaggagg atatttgccc tccggaccag
    36181 gaaagtcttc gggatatgac tatgaaaaaa ctgttaatta tcattttgcc ggtccttctc
    36241 tccggctgta gcgcctttaa tcaactggtt gagcgaatgc aaaccgatac gctggaatac
    36301 cagtgtgatg aaaaaccgtt gacggtcaaa ctgaataatc cgcgccagga agtcagtttt
    36361 gtttacgata atcaactact gcatctcaaa cagggccttt cagcctctgg cgcgcgttac
    36421 tccgacggga tctatgtttt ctggtcgaaa ggtgaggaag cgactgtcta taaacgcgac
    36481 cgcatcgtct tgaataactg tcagttacaa aatccacagc gttgagattt ttccaggggc
    36541 ggcgcacaat agcgtcaccc acagacaatc cgtaaagaaa accatgtctg ataacgacga
    36601 attgcagcaa atcgcgcatc tgcgccgtga atacaccaaa ggcgggttac gccgccgcga
    36661 tcttcccgcc gatccattaa ccctttttga acgttggctc tctcaggctt gtgaagccaa
    36721 actggcggac cctaccgcga tggtggtcgc taccgtggat gaacatggtc agccttatca
    36781 gcgcatcgtt ttactcaaac attacgacga aaaaggcatg gtgttttaca ccaacctcgg
    36841 tagccgtaaa gcacatcaaa ttgaaaataa tccgcgcgtt agcctgctgt tcccgtggca
    36901 tacccttgaa cgccaggtga tggtgatcgg taaagcagaa cgactttcga ctctcgaagt
    36961 gatgaaatat tttcatagcc gcccgcgtga tagccagatt ggttcatggg tttcgaagca
    37021 gtccagtcgc atttctgccc gcggtatcct tgaaagtaaa ttcctggagc tgaagcagaa
    37081 gtttcaacaa ggcgaagtgc cattgccgag cttttggggc ggttttcgcg tcagccttga
    37141 acagattgag ttctggcagg gcggtgagca tcgcctgcat gaccgctttt tgtaccagcg
    37201 tgaaaatgat gcgtggaaga ttgatcgtct tgcaccctga aaagatgcaa aaatcttgct
    37261 ttaatcgctg gtactcctga ttctggcact ttattctatg tctctttcgc atctggcgaa
    37321 aagtcgtgta ccggcaaagg tgcagtcgtt atatacatgg agattttgat ggcaagcagt
    37381 aacttgatta aacaattgca agagcggggg ctggtagccc aggtgacgga cgaggaagcg
    37441 ttagcagagc gactggcgca aggcccgatc gcgctctatt gcggcttcga tcctaccgct
    37501 gacagcttgc atttggggca tcttgttcca ttgttatgcc tgaaacgctt ccagcaggcg
    37561 ggccacaagc cggttgcgct ggtaggcggc gcgacgggtc tgattggcga cccgagcttc
    37621 aaagctgccg agcgtaagct gaacaccgaa gaaactgttc aggagtgggt ggacaaaatc
    37681 cgcaagcagg ttgccccgtt cctcgatttc gactgtggag aaaactctgc tatcgcggcg
    37741 aacaactatg actggttcgg caatatgaat gtgctgacct tcctgcgcga tattggcaaa
    37801 cacttctccg ttaaccagat gatcaacaaa gaagcggtta agcagcgtct caaccgtgaa
    37861 gatcagggga tttcgttcac tgagttttcc tacaacctgt tgcagggtta tgacttcgcc
    37921 tgtctgaaca aacagtacgg tgtggtgctg caaattggtg gttctgatca gtggggtaac
    37981 atcacttctg gtatcgacct gacccgtcgt ctgcatcaga atcaggtgtt tggcctgacc
    38041 gttccgctga tcaccaaagc agatggcacc aaatttggta aaactgaagg cggcgcagtc
    38101 tggttggatc cgaagaaaac cagcccgtac aaattctacc agttctggat caacactgcg
    38161 gatgccgacg tttaccgctt cctgaagttc ttcaccttta tgagcattga agagatcaac
    38221 gccctggaag aagaagataa aaacagcggt aaagcaccgc gcgcccagta tgtactggcg
    38281 gagcaggtga ctcgtctggt tcacggtgaa gaaggtttac aggcggcaaa acgtattacc
    38341 gaatgcctgt tcagcggttc tttgagtgcg ctgagtgaag cggacttcga acagctggcg
    38401 caggacggcg taccgatggt tgagatggaa aagggcgcag acctgatgca ggcgctggtc
    38461 gattctgaac tgcaaccttc ccgtggtcag gcgcgtaaaa ctatcgcctc caatgccatc
    38521 accattaacg gtgaaaaaca gtccgatcct gaatacttct ttaaagaaga agatcgtctg
    38581 tttggtcgtt ttaccttact gcgtcgcggc aaaaagaatt actgtctgat ttgctggaaa
    38641 taatgcatta agtggaaagg gggagtgaga aatcactccc cctggttttt atacagggaa
    38701 catgatgaaa aatattctcg ctatccagtc tcacgttgtt tatggtcatg cgggtaacag
    38761 tgcggcagag tttccgatgc gccgcctggg cgcgaacgtc tggccgctga acaccgttca
    38821 attttctaat cacacccaat acggcaaatg gactggctgc gtgatgccgc ccagccattt
    38881 aaccgaaatt gtgcaaggca ttgccgccat tgataaatta cacacctgtg atgccgtatt
    38941 aagtggctat ctgggatcgg cggagcaggg tgaacatatc ctcggtatcg tccgtcaggt
    39001 gaaagcagcg aatccgcagg cgaaatattt ttgcgatccg gtaatgggtc atccagaaaa
    39061 aggctgtatc gttgcaccgg gtgtcgcaga gtttcatgtg cggcacggtt tgcctgccag
    39121 cgatatcatt gcgccgaatc ttgttgagct ggaaatactc tgtgagcatc cggtaaataa
    39181 cgtcgaagaa gcggttctgg cagcgcgcga actcattgcg caagggccac aaattgtatt
    39241 ggttaaacac ctggcgcgag ctggctacag ccgtgaccgt tttgaaatgt tgctggtcac
    39301 cgccgatgaa gcctggcata tcagccgtcc actggtggat tttggtatgc gccagccggt
    39361 aggtgttggt gatgtgacga gcggtttact gctggtgaaa ctgcttcagg gggcaacgct
    39421 gcaggaggcc ctggaacatg tgaccgctgc agtctacgaa atcatggtga ccaccaaagc
    39481 aatgcaggaa tatgagttgc aagtggttgc tgctcaggat cgtattgcca aaccagaaca
    39541 ttacttcagc gcaacaaagc tctgaaatat ctcatttcgg cccacaacga actgtgggcc
    39601 gacggctatt actttaagcc ttccgctgac agcgcgtctt gtacttccgg acgttcagcc
    39661 atacgttgca taaatgccgc aatgtgctct aacccttcca gattcagttt caccgcgtat
    39721 gcccagcgca gtaccgtaaa cagataggca tcagcaattg taaatctttg cccgcagatc
    39781 cagtgctcat ccttcagtgc ctcgttcaca tattgcagtt tcttctccag ctgcgcgcga
    39841 actgtcggtt tgtactcttc cggtgtatca gggcgaaaca gaggtgtgaa acctttatgc
    39901 agctcggtgg cgatgtaatt cagccattcg atggttttat agcgggaaat actgtttacc
    39961 ggtgccagca actggcggtc ggggacgctg tcggcaagat actgcataat cgctacacct
    40021 tccgtcagca aagtaccgtc atccagcagc aatgcaggta cctgtccctt agggttaacg
    40081 gcaaagtaat cgtcaccgtt ttcgagacgt tttttcatta aatccacact gacgagcgta
    40141 aaatcctttc cgctctcacg cagggtgata tgggaagcga gagagcaggc gcccggtttg
    40201 tagaacaatt tcatcggtaa ctccttttgt ctgtggttta aagtatggta gtgcgttgct
    40261 gaacaaaaaa aaagccgcta acgaaaagtt agcggcttgt aaaagagttt ccctgaattt
    40321 tacgctacgg ctgctttcgc cgctttgtct gcagcgtcat cctgcgtcat gcggtgcagt
    40381 ttcggcgcgg tcagcagcat cagtactgca ataacggcag tagcgacacc aatctgcaag
    40441 aatacgcgac catagacttc cagtgacatc agcggatcgg taacgttatc cggcacagcc
    40501 atcatacccg caacataacc accaatcagg tttgcaccgg cagtggtcag gaaccagcta
    40561 cccataatga agcccatcag acgctgcgga acgagttgtg caaccattgc cagacccaga
    40621 ccagagatca tcagttcccc gatactctgc aggccatagc tcgcgaccag ccagcttaca
    40681 gacacgatac ctgcgtcaga cgcgaatttc gcacccagcg gcagaatcag gaacgcacca
    40741 gaacacatca ccataccgat tgcaaacttg gttggcatcg gcagggtatc gcccatcttg
    40801 ttatagatag cggccagaat cgggctacca atgatgatcc agaacgggtt cagtgcctga
    40861 tactgctcag gttctacggc caggcccaga atggtatgtt caacgttacg aatcgcaaag
    40921 aagttcagtg acgttggcat ctggctgtac agcacgaaga agataatggc ttcgagcatc
    40981 aggatgaagg caacgatcat tttacgacgc gcagcacctt tcatcgcgaa ggcttcttta
    41041 ccgaagataa ccacgatacc gaaggcaaca acgcccagcg ccatacgcgc aacttcctga
    41101 ttgtgcagca gccaggtggc gatagcgatc agtgccacaa caccaataat ggtcagcagc
    41161 aggttacggt agttgatagg ctcgaagtct ggttttgaac cgttctgttt aacccagcgt
    41221 tggcagaagg cgaagttaac gatagtgatc agcaggccta caacgctcaa cgcaaacgca
    41281 acactccagc cgtatttcgc ggccagccac ggggtggcga tcatagagaa gaaagagccg
    41341 atgttgacgg acatgtagta catggtgaat gcaccgtcca gacgcgggtc gtttttctca
    41401 tagcatgtag aaagcagaga agacgggtta gctttaaaca ggccgttacc gaccgcaata
    41461 gccgccatac ccatataaac gataccggcg tcgtgaccag accaggcaac cagagcataa
    41521 ccaatcgcca gcacaatagc gccgagcata attacgcgtt tagtacccag taccttgtca
    41581 cctaaccagc cgccgatagc gaccagacca taaaccaggg cactaaagga agagaaaagg
    41641 gtgattgaat ccgcttcaga catacccagt tgtttaacca ggtaaacagc cataattcct
    41701 tgtaggccgt aataaccaaa acgttcccat aactcaatcg agaagatgag atagaacgcc
    41761 ttcggttgtt tgaaagcgtt caaactgacg ctttcagttg gtttttggtt tgcagtggac
    41821 acttttacct ctttttttac atcccatatt aacgggggtg ctttctcgcg ttatgtctaa
    41881 aaaacatacg cgcaattgtt atagtgggag gggaaacggc aggtaatgtt cactatcttg
    41941 cactttcaga caagtccttt gtaataatct gttacatata actgaggcgg ggaattcaac
    42001 cattccggga aatgttatcc agcgttaaat ttttcaaaaa acgtaacggc agattttttc
    42061 actgcattga gagggggatg atggcttgac ggcgatatgt tctgctattt agcgtttttc
    42121 ctagtgagat agtccagttt cttaaaaata gccagtgtaa tgttttgtag gtcaatattt
    42181 gttgcgtgtg taaatttaaa tgattttaac ctgattcttg caatgttttt acattgccac
    42241 agcatcgtga tcttgatcac taatgaatag aaaatttcgc tattaaaaaa acaattaacc
    42301 tgcatttttg gttatccgca aacggattac ctgatgataa cgctaagcaa tggcatcatt
    42361 gacgttaaaa agatgtttta aaagaggata aagaaaggtt atcaatgggg taatcggtgt
    42421 tacccctttt cttcagatgt caactttctc tttgtattca caaagatctt caataataca
    42481 agagccacag cggggcttgc gggcaatgca ggtataacgc ccgtgcagga tcaaccaatg
    42541 gtggcagtcg actttaaact ctgccggaac cactttcagt agcttttctt ctacctgttc
    42601 gacgtttttc cccggcgcaa attgagtacg attacaaacg cggaaaatgt gcgtgtcgac
    42661 agcaatagtc ggccagccga atgcagtgtt taatacgacg ttggctgttt tacgacctac
    42721 gccgggcagg gcttcaagcg cagcacgatc ttccggaacc tcgctattat gctgctccag
    42781 taagatacgg caggttttga tgatattttc tgctttgctg ttataaagcc caatcgtttt
    42841 gatataggtt ttcacccctt caacgcccag ttcaagcatc gctgcaggcg tattcgccac
    42901 cgggtagagt ttcgccgtcg ccttattaac actgacatcg gttgcctgag cggaaagcag
    42961 tacggcaatc agcaattcaa aaggcgaact gaaattaagc tcagtggtgg gatgaggatt
    43021 gttctcacgc aggcgagtga ggatctccag gcgttttgct ttgttcatca gacattccct
    43081 gtttcaccgt ttggcaatgc acgttcggca gctgcttcag tacggcgctt tttcattctt
    43141 tcatcaatca ggtattttcc tgccagcatc agtcccaggc caataaatgc acctggtggc
    43201 agcatcgcca gcaggaaagg ggagtcggtg tggaaaatct ccacgcgtaa tacttttgcc
    43261 cagctaccta acagcgcatc tgcaccgtca aacaatgtgc cattgccgat aatttcgcgt
    43321 agtgaaccca gcacgaacat ggcgcaggtt gcgcccatac caattgaaaa gccgtccagt
    43381 gccgaaagcg ccggaccttt tttggcagcg aaggcttcag cgcggcccac aacgatacag
    43441 ttagtgacaa tcagcggaat aaaaatccct aatgattgat acaggccaaa ggcgtaggcg
    43501 ttgatcagca tctgtacagc gctgaccacc gaggcgatga tcatcacgta aatgggaatg
    43561 cggatctcgg ctggcgtcca gtgacgcagc gtcgaaatgg tcaggttggt cagcgtcagt
    43621 accagcgtag tcgcaagtcc taaacccaga gcgttagtgg cagtagacgt gacagccaac
    43681 agaggacaaa ggccgagcaa ctggaccagc gcagagttgt ttttccacaa cccctgaaca
    43741 ataacgtctt taatttcgct cacgctttat tctccacagg caggaagttg agaaagttgt
    43801 tccggtaacg tctgagcgta caatccggcg cgttttaccg cattaaccac cgcgcgggga
    43861 gtaatcgtcg cgccggtgaa ctggtcgaaa ttaccaccat ctttcttcac cgcccagtgc
    43921 gcatcatccg caccactgat ttttttaccc gcaaaatggg tgatccagtc agaaaggcgc
    43981 agttcgattt tatcgccaag ccctggcgtt tcgtggtgct ctgtcacgcg cgtgccaagt
    44041 accgtgccgt taaaatcggc tcccaccagc agctgaatcg caccggaata gccatctggc
    44101 gcggttgctt ccagaacggc ggctaccggt ttgtcatcct gtttggcgat gtaaacccga
    44161 tgctcacctt tacctaactc tggcgcagtt accagatagc aactctgtgc cagcgcattg
    44221 ttatagcgtt cggctggcag cacctgatca aataaagcct tttgttgcag actggcctgt
    44281 tcagcaatcg tcgttttggt catctggttg atggccgcag ttaaccctgt tgaacccgct
    44341 gcaaatagcg ccaacgtaat gccgtgtttt cggatagttt tcagcatggt ttaccctttg
    44401 cgatggccgt agacgcgcgg acgcgtgtag taatcgatca gaggaaccgt gatgttcgcc
    44461 agcaggacgg caaaagccac gccgtcagga tagccgccga aactgcggat caaccagact
    44521 aataagcccg caagcgcgcc gaaaataaga cgaccacgat tggtcgtaga agcggtaacc
    44581 gggtcagtca aaataaagaa tgcgccgagc atggtcgctc cagacagcag atgaatttgc
    44641 ggtgctgcca gtgtttctgg tgagaacaac cagcccaacg ttgcgcataa cgccagcgtt
    44701 actaagaagc tgaggggaat atgccagcga atcgctttct gccatagcaa ccatacgccg
    44761 ccagccagcc aggcgagatt tacccattgc caaccagcgc ccgccagaat accgctgtag
    44821 atcggatatt gcataatctg ttcaaccgaa tgaccggcac ggacagaggt tttaaatgta
    44881 tccagcggtg tcgcctgact aatgccatca atacctaagc gtagtgtgtt catatcacca
    44941 ccactggcgg tatgcccgct gaaaataacc tgtatggcgt cgataaaacc agggatgttg
    45001 accgcaattt catgtggcgg taaccagctg gtcatctgca ccgggaagga gatcagtaag
    45061 accacataac caatcattgc cggattaaac gggttttgcc ccagaccgcc atacaactgt
    45121 ttagcgatga taaccgcaaa caccgtaccc agcacgacca tccaccatgg cgcgaggggg
    45181 ggaatactta ccgccagcaa taagcctgtc agcaatgctg agttatcttt caacgtggcg
    45241 gctaccgact gcttgcgtag tttgagtacg agagcttcgg ctaacagagc actaaccgat
    45301 gccaacagga tctgaacgag agtaccccaa ccaaaaaacc acagttgcgc tgcgattcct
    45361 ggcacggctg cgagcaacac caacagcata atgcgcgatg tctggcgctg gttatgggta
    45421 taaggggagc tagctattct gaataccatt tagtcctcgt ttacaacctt ttgctggacg
    45481 gcttttttgg cctgaacgcg ggcaatagcc gcggcaactg ccgctttgcg cggatcaacc
    45541 tgttcttctg gttctgcatt agcctgttgc tgttccagct tgcgtgcttt ggcgcgtgca
    45601 atagccgctg cgacggcagc tttgcgcgga tcaacctgtt cttctggctc cgcattagcc
    45661 tgttgctgtt ccagtttgcg cgctttggca cgggtaatag cggcttcgac ggcggctttg
    45721 cgcggatcga ccggttcttc tggttccgca ttagcctgtt gctgttccag cttgcgtgct
    45781 ttggcacggg caatagcggc ttcgacggcg gctttgcgcg gatcgaccgg ttcttctggt
    45841 tccgcattag cctgttgctg ttccagcttg cgcgctttgg cgcgggcgat agcggcttcg
    45901 acggcggctt tgcgcggatc gacctgttgt tctggttccg cattagcctg ttgctgttcc
    45961 agcttgcgtg ctttggcacg ggcaatagcg gcttcgacgg cagctttgcg cggatcgacc
    46021 tgttgttctg gttccgcatt tgcctgttgc tgttccagct tgcgcgcttt ggcgcgggcg
    46081 atagctgctt caacggcagt tttacgtgga tcagtaacgg ttgctgcgtc gttagtttgc
    46141 tgcagttctg cctgtttcgc tctggcttgc gctttacggg cttcccgtgc tgcaataatt
    46201 gcactgttat ccgggcgttc gcccgcttta atcacaatag gctgtgtagc ctgggcctgt
    46261 ttctctttca cccgcgccag agcggcagca atcgcatctt tatctttggc tgcaggttga
    46321 acggctgcgc tcttatgtcg ttcaaggcga gccgcttttt cgcgctccag acgagcctgg
    46381 cgcgcttcga aacgcgcttt ggcttctgcg gcgcgctttt cttcctgacg aatagccgca
    46441 atttcagctt tttcctgacg gaaatattgc accaggggaa tattgctcgg gcaaacccac
    46501 gcgcaagccc cacattcaat gcagtcggca atgttatgcg tggtagcttt atcgtgttgc
    46561 tgacctttgc tgaaccagta caactgttgc ggcaaaagat ccgcagggca ggcgtcagca
    46621 caggcgctac accggatgca gctttgttct tcctgtggtt cgccaagctc attggcagag
    46681 ggagccaaca gacagttggt aatctttacg accgggacat ccagccatgg caaggtaaag
    46741 cccattagcg ggccgcccat aatcaccatt tgatcggcag aggggcagaa tccggcgtca
    46801 ttcaataaat gacgcactgg cgtccccagc cgtgcccaga cgttgcccgg gcgagcgatt
    46861 gcttcgccag tcagggttac gacacgctcg gtaatcggct cgccatcaat aacggcacgt
    46921 ttcactgcat aagcagtgcc gacgttttgc attaatacgc cgatatcgga tgaacgcccg
    46981 ccatgtggaa cctgcttccc ggtcagaatg taggttaatt gtttagcacc gccagaagga
    47041 tatttggttg gaatcacccg catagaaata tcgtgagagt ccgccagcac cgcgcgcagc
    47101 atggaaatcg cctgcggttt gttatcttca atgccgataa gaatttcgcg tggctgcaga
    47161 atatgcgcaa gaatgcgaat accctctacg acctgagccg cgcaatcctg catcaaacgg
    47221 tcatcggcgg taatgtatgg ctcgcactca gcagcgttga taatcaacgt ctcaatctta
    47281 tctccgccgc cctgcaattt aacgcctgtc gggaatcctg caccacccag cccggcaaca
    47341 ccaaactgat gaatgcgctc gattaactct tcgcgacggc gagagcgata atcggcccag
    47401 ccgtctcgtg ggatccagca gtcttcacca tcggcatcaa taatcacgct taattcagct
    47461 aaagctgaag gatgagccgt agagtggggc gcaatagccg taacggtacc cgaggtgggc
    47521 gcgtgaacag gcagcatttt gccgcgacca cgggtaagcg gctggccgcg caatacttta
    47581 tcgccgacgc taacgcacaa ctcaccttca gcgccaatat gctgtttcag tggaatgaca
    47641 aaacgctgcg ccagtggtac ctggcgcagg ggtgtaccgt tggactgggt tttcatctcc
    47701 ggtggatgga tgccgccgtt gaaatcccag attttatttt ttctgaatgc agagaataac
    47761 ttaagcatgg tgttccacgg gaatgatacg cacgggaatg gtgttcagat cccatttcca
    47821 ggagtcaggt gtttctgcga ccggttgcaa cgagatgcag tgcgtcgggc acggatcaac
    47881 acataaattg cagcccgtac agagatcact cattaccgta tgcatggcac gggtagcgcc
    47941 aacgatggcg tctaccggac acgcctgaat acatttagtg cagccaatac agttattttc
    48001 atcaataacc gccaccatcc gcgcaggcgt tagctcttgc gcttcgccat ccagcggctg
    48061 cggctcgaca ttaagcaact cggcaatttt tagcatcaca gcttcgccac caggggcgca
    48121 acggttgatt ttttcaccgt tacagctgat ggcttccgcg taggggcgac agccgggata
    48181 accgcactga ccacactggc tctgcggtaa gatttcgtca attttctcaa cgaccggatc
    48241 gtcttccacc gcaaaacggc gggaggcata acccagaatg gcgccaaacg ccaggcccag
    48301 caggctcacg gcggcaacgg caatccagat agcattcatt acaacttcac caaaccacta
    48361 aagcccataa aggccagaga cataagacct gcggtaatta acgcaatggc attaccgcga
    48421 aaaggtgccg ggacatcagc cacagcaagg cgttcgcgga tggctgcgaa gagaaccatc
    48481 accagcgaaa aaccgacagc ggcggaaaaa ccgtacagcg ccgactgcaa gaaattgtgc
    48541 ccgagattga tattcagcaa cgccacgccg agcacggcac agttggtggt gataagcggc
    48601 aaaaaaatcc ccagcaaacg gtaaagcacc gggctggttt tgcgcaccac catctcggtg
    48661 aactgcacga ccacagcaat caccagaata aatgccaggg tgcgcaggta aatcagatta
    48721 agtgggatca aaatccacgt atcgataagc caggcgcaaa tagacgccag cgtcatcaca
    48781 aacgttgttg ccagccccat gcccatcgcg gtttccagct ttttggaaac ccccataaac
    48841 ggacagagac cgagaaactt gaccagtaca aagttattga ccagtacagt tccgacaaac
    48901 agtaacaggt agtcagtcat ttttcggcct gaaataaaaa agccgcctat tatccgttaa
    48961 tccagagcag gcgacaacag gttaactgta aggttattac ggattcacaa acgtcgcttt
    49021 cacccgcgac gagcgtttga aataggggac gatcaatgcg gttgccaaca aagtaaacag
    49081 caactggcga acggctatgc catcttccac gggtgaaaaa gcgaacgctt ttaccgccag
    49141 caatacggaa ataagcagcc agataatata gtgtttagga acacaacgcc gacgtttaaa
    49201 gaaagcgatg gtcaaccaca gtgtgtaata ccacatggcg atagcggtaa taaaagaaac
    49261 gccccataag atttgtgtcg tcagtgcttg tgcgccgagc gtttgaaatg tttgcggaga
    49321 agataacgca gcggtataca gcaacaacgc cagcgtcgta cttaacagag cgactaaaag
    49381 ccaggccaac gggccaagca accagcctcc aatacgttgt ggcgttgtgg tggtcatagg
    49441 ttctccagaa tagcgacgca aacaggcaaa tctaatttta ggggcgttat tataaggctt
    49501 ttataaccga tcgctacttt ctttattgaa catagtgcca gacggacttc ggtacctggc
    49561 cgaggtcaaa caaacgcccg ccagaaacca gctctgcgcg acggtgatcg gcagcacgat
    49621 acatattgat cagttcctga tcgtccgtca gggtgtaatt aagatggtcg tagagttttt
    49681 ccagactttc gagtgaactg attttgcgaa attttaataa gtagtcctga acagtcataa
    49741 aaggtccata aaataattaa taccaaaggg gtaggtatta ttagtgatga taaaatttca
    49801 ccaaaagtga acgcttgtgt tattacaata ataagaatgc gctgatggca aatattcagg
    49861 gatgaaaaac tgctatgaaa agagtaacgc gctttactat ccctgacaat caccaacaac
    49921 accgaacaag ataataaatt cctggtttaa tatccgacaa gtgaaaacat gcaccctgac
    49981 gggcagcatg tcgctctaca agcgcagggc ttacttgtgt tgtaccgtag tactctgttc
    50041 aggtggctga tagttgtcaa tgtgactcgc cacgccaaga agaatgactg aaacgacaag
    50101 aacgatccaa cctgttaatt caataagacg attcattaca actcacatct ctgttgattg
    50161 atccattaac ttcagggggt aaatgttact tagcaataat agctccagca agatttttac
    50221 tgaggttttt gcgatattta gcttttgtcg ttggaaaaat cgctattttt ttgacttaag
    50281 ttaaacaaca tcccttattg ctggcaggtt attaaactgt tgagcgaggg taaaggatag
    50341 tgtcaaatag ccatcatact tcaatgagag gcaatgacat gagcgacaac atccgtgttg
    50401 ggttgattgg gtatggttat gcgagcaaaa ccttccatgc gcccctgatt gcgggcacac
    50461 ccgggctgga actggcggta atctccagca gtgatgaaac aaaagtaaaa gccgactggc
    50521 caacggttac ggttgtctct gagccgaagc atctgtttaa cgatcccaac atagacctga
    50581 ttgtcattcc tacacccaac gatacccatt tcccgttagc caaagcagcg cttgaggcgg
    50641 gtaaacatgt ggtcgttgat aaacccttta ccgtgacact gtcacaagcg cgagagctgg
    50701 atgcgctggc aaaaagcctg gggcgtgttc tgtctgtatt ccataaccgt cgttgggata
    50761 gcgatttctt gacgctaaaa ggtttgctcg cggaaggcgt gctgggtgaa gttgcttact
    50821 ttgagtctca ttttgaccgc ttccgtccgc aggtgcgcga tcgttggcgt gaacagggcg
    50881 gcccaggcag cggtatctgg tacgatttag caccacatct tcttgatcag gccattatgc
    50941 tatttggttt accggtcagc atgacggtag atttggcaca gttacggccc ggagcgcagt
    51001 cgaccgatta tttccacgcc atcttgtcct atccacagcg gcgagtcatt ttacacggta
    51061 ccatgctggc agccgctgag tcagcacggt atatcgtgca tggatcccga ggcagttatg
    51121 tgaaatatgg cctcgatcca caggaagaac gtctgaaaaa tggcgagcgt ctgccgcagg
    51181 aagactgggg ctacgatatg cgtgatggcg tacttacccg cgtggaaggt gaggaacgtg
    51241 tcgaagaaac gctgttgacg gtgcctggga attatccggc ttactatgcg gcgattcgtg
    51301 atgcgttaaa tggcgatggt gaaaatccgg ttccggcaag ccaggcaatc caggtaatgg
    51361 agttgattga gctgggcatc gaatccgcca aacatcgcgc gactttgtgc cttgcatgaa
    51421 aaatggatgg tgcaatttgc accatccatt tttgttactt cgctgcgact ttttctcgca
    51481 gtgcgcgttt ttcctctgcg ttgaggaaag ccatttccag accattaatc tgtgcctggc
    51541 ggatttgctc gcgggataac ccagcagctg gcgcggcaac ggtatattcg tgaatgatat
    51601 ccactccctg tacgccggga tcgtcagtgt taatgctggc acgaatgcca tgctcaagga
    51661 acgttttcag cggatgtgca gccagctctg ccacggtgct ggtctgaata ttggaggtca
    51721 gacaggattc aataccaatt tgttgctcgg cgagaaaatc catcagcgcc cgatcttcaa
    51781 tggcttttac gccatgtcca atgcgctctg cccccagttc acgaatcgcc tgccagatgc
    51841 tttccggccc ggcagcttcg cctgcatgga cggtaatatg ccagcccgca tcacgcgcgc
    51901 ggttgaagtg agaaagaaac agacttcccg ggaaaccaag ttcatcaccg gctaaatcaa
    51961 gtgcggtaat ctggtcacgg tgggctaaaa aggcctccag ctcttgctga caggcggctt
    52021 cgccgaaggt tcggctcata atgccgataa gcttcgcctg cacaccaaag gtgcggcaac
    52081 cttcacgtac gccatcgatc accgcttcaa caacacctgc aacaggcagc tgatgtgcca
    52141 ttgccatgta gcctggtgaa aaacgcagct cgacatagtg caggccgtta cgggctgcat
    52201 cttcaatgtt ttcaaatgcc acgcggcgac aagcatcaag agaggcgaga actttaacgc
    52261 cccagtcaag tttggtcaga aagctcacca gatcgggttc gttggcaatg acctgaacgt
    52321 ggggaatcag tgtttccagg gattgtgcag gaagcgagat attatactgg cggccaagtt
    52381 caagaatggt ctggggacga atgttgccat caaggtggcg atggatatca gttaatggca
    52441 gggtggtatc aatcatggtc gcactctttt tctggatata aaagtgcgca ccattataaa
    52501 aacaaagcag ggtaaaaatc cacgattgcg caacgatttt ttacgttgcg caattggggt
    52561 tagcgaacag cgcggatggc gttaattaat ccagccacac ctttttccag tttcgaacgt
    52621 gggcagccgg cattgagtcg gacaaaacca cgaccttctt caccgtaggt ataccccggc
    52681 atgatcgcga ctttttcttg ttcgataagt gctttttgca acgcgttgtc gtcaatattc
    52741 aacggacgta aatcaagcca ggccagataa gtggattgcg ggatctgcca gttgagttca
    52801 ggaaacgcgg cgttcatttt atctgcgata tacgtcaggt tatctttcag atagacgcgt
    52861 aaggcatcca gccacggtgc accttgctga taggcggcga tatgggcagt taacgccagt
    52921 accgaagggg aagaaagccc atcacgtcct ttcagtgccg ataaataggc atcacggctg
    52981 ctgctatttt ctataatccc gtaagcaccg gtcagcgcgg gaatattgaa acttttcgat
    53041 cccgacgtca gcaacgccca gtctccccgc gccacattac tccagggaat atgcggctgc
    53101 tcgccccaaa ccatatccat atggatttca tcggaaataa cccgcacacc atgacgctcg
    53161 cacaggtcag ccatgatctc cagctcatcg cacgtccaca cttttccggt aggattctgt
    53221 gggctacaca ggagcataat tttacattct ggtttcgcca acacggcttc cagcttgccc
    53281 atatcgcaaa accagccgtc agcctgcttc tctaaagcaa cgggcattac tgtgcgctga
    53341 ttaccttcaa tggccttgta aaatgcgtca taggcgggtg tgtggatcac cacgccttca
    53401 cctgtttcag accactgacg aatcagttct gagaccatat agataacaga agggccatac
    53461 accaccgact gaggatcgat ggcggtgtaa tgctgggtgg aaaaccagtg ggcaatagcc
    53521 gcgagaaact catcgttctt ccagcggctg tagccaaaca cgccgtgcat caggcgctga
    53581 ttcagcgcct cgataatgca gggggcagtg gcaaaatcca tgtctgaaat cgtgaaaggt
    53641 aacaggtcag cagtgccgaa acggtcagcg acataatccc actgcgtaca ccatgtgcca
    53701 tgacgatcca cgacctttga aaaatcgaac atatcttatc cttatgcctg gacagtatgc
    53761 atcagaccgg ccatttcatc ttttactgac tgaacttgtg gcccgataac aacctgcagg
    53821 ttatgttgat taagttgtac tacgccaatt gcccgattgt ccttcagtgc ctgcacatta
    53881 acaagcgaca tatctttcac agacaaacgc agacgggtaa tgcagttatc gaggctaaca
    53941 atattgtcgg caccgcctaa ggcttcgagg attgccggaa cgttgtaacc tgatttaccc
    54001 ggcgcaccgg caacggcttt ttcgattgaa ctggccactt cgctatcgcg ccccggggtt
    54061 ttcagattga agcgggtgat agcgaaacgg aagatgacgt agtaaacgac aaaccagatt
    54121 gccaccacca ctggcaccat gtaccacttg gttgacagac catgcaaaat accgaacacc
    54181 acgaagtcga tgatattgcc gtcggtatta ccgatggtga cgccgagcac agacatgacg
    54241 gtgaagccga ggccggttaa cagcgcgtgg atgacataca gaactggcgc tacgaacagg
    54301 aacaggaatt ccagcggttc ggtcgtgccg ccaacgacac aggcgatcag gccagaaatc
    54361 agcagccctt taattttatg gcgattttcc gggcgcgcgc agtgatacat cgctaacgct
    54421 gcacctggca gaccgccgag aaacgcaggc attttgcctt gcgaaaggaa acgcgtggcg
    54481 ctttcagaaa aaccgtgagt ggtcgggcaa ctcaattgcg cctggaagat ggtcaatgcg
    54541 ccgctgacgg tttgaccgca gacttcctgc gtgccacctg cgtcggtaaa gcgaattaat
    54601 gccaccagaa tgtgatgcag accaaacggc aacagcagac gttcaccggt accaaacagc
    54661 atcggtccga aatcacccgc gctattgatc atatggccta agccgctaat acccatggcg
    54721 aaaatcggcc agactaatgg aatcaccagg ccgacaaggc ccatcaccag cgaggagatg
    54781 attggtacga agcgcgtacc gccgaagaat gccagcgcat ccggcaggcg gatattatgg
    54841 aatcgctcat gcagcatcca gacgataata ccggcgatca ccgcaccgag gatcccggtg
    54901 tcgatcgact ggatcccaag aatgctctgg atgttattgg ctttcagaac ggcggcatcc
    54961 gtggttggca gaatgccttt attggtcaac cagaagttta ccgcgaggtt cattaccgca
    55021 taaccgacga agccagcgaa tgccgctacg cctttgtttt ctcgtgccag acccagcggg
    55081 atggcgatac agaacatgac tggcaggaaa ctaaaagcaa acgagccaat cttactcatc
    55141 caggtaaaga tagcctgcaa cacggggttg cccaggaccg ggagcagggt gatgacatcg
    55201 tggctactaa gggaactacc aatgccgagc ataatgccgc agaacgacaa taatgccacg
    55261 ggtaacatga aggttttgcc taactgctgg aagaactccc acagcgtgac tttcggtgct
    55321 gttttcgccg tcataaaacg actcctcgta gagaacaatc tggattcagt aaattgcgcg
    55381 agaagataaa acgttttatc aaattttagt gaggcataaa tcacattacg caacgataat
    55441 agcgggtata agataaataa aaggtaaaac gttttatctg tcacaaaatc agggagtagg
    55501 tcatctgcat ggctaccgcc aaaaaaataa ccattcatga tgttgcgctg gctgcgggcg
    55561 tgtcggtaag taccgtttcg ctggtgctta gtggcaaagg gcgaatctct accgccacag
    55621 gagaacgcgt taacgccgcc attgaagagc tgggatttgt gcgcaatcgc caggcgtcgg
    55681 cgctgcgcgg tgggcaaagc ggcgtcattg gtttgatcgt ccgtgattta tctgcgccgt
    55741 tttacgccga attgacggcc ggattgacgg aagctctgga agcgcaggga cggatggttt
    55801 ttttggttca cggcggtaaa gacggcgagc agctggcaca gcggttttca ctgttactga
    55861 atcagggtgt cgatggtgtg gtaattgccg gggctgcagg aagcagcgat gacctgcgac
    55921 ggatggcaga agaaaaagct atcccggtga ttttcgcttc ccgtgccagt tatcttgatg
    55981 atgttgatac ggttcgcccg gacaacatgc aggctgcaca gttgttgacg gagcatctca
    56041 ttcgcaatgg gcatcagcgg atcgcctggc tgggagggca aagttcctca ttaacccgtg
    56101 cagaacgggt ggggggctat tgtgcaactc tactaaaatt tggcctgccg tttcacagcg
    56161 attgggtgtt ggagtgcact tccagccaga agcaagccgc ggaagctatc acggcgcttt
    56221 tacgtcataa cccgaccatc agtgccgtgg tttgctataa cgaaactatt gcgatggggg
    56281 catggtttgg tttgctgaaa gcagggcggc aaagcgggga aagcggagtc gatcgttact
    56341 ttgagcaaca ggtttcgctg gcggcattta ccgatgcgac accaaccaca cttgatgata
    56401 tacccgttac ctgggccagc acgcctgcgc gggaacttgg taccacactt gcggatcgca
    56461 tgatgcaaaa aatcacccat gaagagacgc attcacgcaa tcttattatt cccgcccggc
    56521 tcattgcggc gaaataatcc tctctttatc tgctatacct ggtagtgtcc tttcctcaag
    56581 tttaatgata aaaaatgtgg cacacaggcg gtgcctttta tttttactga agttcacagg
    56641 aggtttatgt gtttaattct gacaacctga gactcgacgg aaaatgcgcc atcatcacag
    56701 gtgcgggtgc aggtattggt aaagaaatcg ccattacatt cgcgacagct ggcgcatctg
    56761 tggtggtcag tgatattaac gccgacgcag ctaaccatgt tgtagacgaa attcaacaac
    56821 tgggtggtca ggcatttgcc tgccgttgtg atattacttc cgaacaggaa ctctctgcac
    56881 tggcagactt tgctatcagt aagctgggta aagttgatat tctggttaac aacgccggtg
    56941 gcggtggacc taaaccgttt gatatgccaa tggcggattt tcgccgtgct tatgaactga
    57001 atgtgttttc ttttttccat ctgtcacaac ttgttgcgcc agaaatggaa aaaaatggcg
    57061 gtggcgttat tctgaccatc acttctatgg cggcagaaaa taaaaatata aacatgactt
    57121 cctatgcatc atctaaagct gcggccagtc atctggtcag aaatatggca tttgacctgg
    57181 gtgaaaaaaa tattcgggtg aatggcattg cgccgggggc aatattaacc gatgccctga
    57241 aatccgttat tacaccagaa attgaacaaa aaatgttaca gcacacgccg atcagacgtc
    57301 tgggccaacc gcaagatatt gctaacgcag cgctgttcct ttgctcgcct gcagccagct
    57361 gggtaagcgg acaaattctc accgtctccg gtggtggggt acaggagctc aattaataca
    57421 ctaacgaacc ggtaaacagc cgtatgtgtt gtttaccggg atagcgtgtg caattcatca
    57481 acgtctctgc taaacaactg gcagccaaat cacggccatt ggttaaccaa tagctggatg
    57541 aaaagcatac gaatagagtg tgccttcgca ctattcaaca gcaatgatag gcgctcacct
    57601 gacaacgcgg taaactagtt attcacgcta actataatgg tttaatgatg gataacatgc
    57661 agactgaagc acaaccgaca cggacccgga tcctcaatgc tgccagagag attttttcag
    57721 aaaatggatt tcacagtgcc tcgatgaaag ccatctgtaa atcctgcgcc attagtcccg
    57781 ggacgctcta tcaccatttc atctccaaag aagccttgat tcaggccatt atcttacagg
    57841 accaggagag ggcgctggcc cgtttccggg aaccgattga agggattcat tttgttgact
    57901 atatggtcga gtctattgtc tctctcaccc atgaagcctt tgggcaacgg gcgctggtgg
    57961 tcgaaattat ggcggaaggg atgcgtaacc cacaggtcgc cgccatgctt aaaaataagc
    58021 atatgacgat cactgaattt gttgctcagc ggatgcgtga tgcccagcaa aaaggcgaga
    58081 taagcccaga catcaacacg gcaatgactt cacgtttact gctggatctg acctacggtg
    58141 tactggccga tatcgaagcg gaagacctgg cgcgtgaagc gtcgtttgct cagggattac
    58201 gcgcgatgat tggcggtatc ttaaccgcat cctgattctc tctctctttc cgcgggctgg
    58261 tgacaactga gcccgcgttt catactttaa tttccctgta caaaatacaa tctcgccgga
    58321 gcggggaatt cctccctaaa atattcaccg tagccatatg tcatgagagt ttatcgttcc
    58381 caacatgctc gaacgaacgt tcggttgctt attttatggc ttctgtcaac gccgttttaa
    58441 agattaatgc gatctatatc acgctgtggg tattgcagtt tttggttttt tgatcgcggt
    58501 gtcagttctt tttatttcca tttctcttcc atgggtttct cacagataac tgtgtgcaac
    58561 acagaattgg ttaactaatc agattaaagg ttgaccagta tttttatctt aatgaggagt
    58621 cccttatgtt acgtcctgta gaaaccccaa cccgtgaaat caaaaaactc gacggcctgt
    58681 gggcattcag tctggatcgc gaaaactgtg gaattgagca gcgttggtgg gaaagcgcgt
    58741 tacaagaaag ccgggcaatt gctgtgccag gcagttttaa cgatcagttc gccgatgcag
    58801 atattcgtaa ttatgtgggc aacgtctggt atcagcgcga agtctttata ccgaaaggtt
    58861 gggcaggcca gcgtatcgtg ctgcgtttcg atgcggtcac tcattacggc aaagtgtggg
    58921 tcaataatca ggaagtgatg gagcatcagg gcggctatac gccatttgaa gccgatgtca
    58981 cgccgtatgt tattgccggg aaaagtgtac gtatcacagt ttgtgtgaac aacgaactga
    59041 actggcagac tatcccgccg ggaatggtga ttaccgacga aaacggcaag aaaaagcagt
    59101 cttacttcca tgatttcttt aactacgccg ggatccatcg cagcgtaatg ctctacacca
    59161 cgccgaacac ctgggtggac gatatcaccg tggtgacgca tgtcgcgcaa gactgtaacc
    59221 acgcgtctgt tgactggcag gtggtggcca atggtgatgt cagcgttgaa ctgcgtgatg
    59281 cggatcaaca ggtggttgca actggacaag gggcaccagc gggactttgc aagtggtgaa
    59341 tccgcacctc tggcaaccgg gtgaaggtta tctctatgaa ctgtacgtca cagccaaaag
    59401 ccggacagag tgtgatatct acccgctgcg cgtcggcatc cggtcagtgg cagtgaaggg
    59461 cgaacagttc ctgatcaacc acaaaccgtt ctactttact ggctttggcc gtcatgaaga
    59521 tgcggatttg cgcggcaaag gattcgataa cgtgctgatg gtgcacgatc acgcattaat
    59581 ggactggatt ggggccaact cctaccgtac ctcgcattac ccttacgctg aagagatgct
    59641 cgactgggca gatgaacatg gcatcgtggt gattgatgaa actgcagctg tcggctttaa
    59701 cctctcttta ggcattggtt tcgaagcggg caacaagccg aaagaactgt acagcgaaga
    59761 ggcagtcaac ggggaaactc agcaggcgca cttacaggcg attaaagagc tgatagcgcg
    59821 tgacaaaaac cacccaagcg tggtgatgtg gagtattgcc aacgaaccgg atacccgtcc
    59881 gcaaggtgca cgggaatatt tcgcgccact ggcggaagca acgcgtaaac tcgatccgac
    59941 gcgtccgatc acctgcgtca atgtaatgtt ctgcgacgct cacaccgata ccatcagcga
    60001 tctctttgat gtgctgtgcc tgaaccgtta ttacggttgg tatgtccaaa gcggcgattt
    60061 ggaaacggca gagaaggtac tggaaaaaga acttctggcc tggcaggaga aactgcatca
    60121 gccgattatc atcaccgaat acggcgtgga tacgttagcc gggctgcact caatgtacac
    60181 cgacatgtgg agtgaagagt atcagtgtgc atggctggat atgtatcacc gcgtctttga
    60241 tcgcgtcagc gccgtcgtcg gtgaacaggt atggaatttc gccgattttg cgacctcgca
    60301 aggcatattg cgcgttggcg gtaacaagaa ggggatcttc acccgcgacc gcaaaccgaa
    60361 gtcggcggct tttctgctgc aaaaacgctg gactggcatg aacttcggtg aaaaaccgca
    60421 gcagggaggc aaacaatgaa tcaacaactc tcctggcgca ccatcgtcgg ctacagcctt
    60481 ggtgacatcg ccaataactt cgccttcgca atgggggcgc tcttcctgtt gagttactac
    60541 accgacgtcg ctggcgtcgg tgccgctgcg gcgggcacca tgctgttact ggtgcgggta
    60601 ttcgatgcct tcgccgacgt ctttgccgga cgagtggtgg acagtgtgaa tacccgctgg
    60661 ggaaaattcc gcccgttttt actcttcggt actgcgccgt taatgatctt cagcgtgctg
    60721 gtattctggg tgccgaccga ctggagccat ggtagcaaag tggtgtatgc atatttgacc
    60781 tacatgggcc tcgggctttg ctacagcctg gtgaatattc cttatggttc acttgctacc
    60841 gcgatgaccc aacaaccaca atcccgcgcc cgtctgggcg cggctcgtgg gattgccgct
    60901 tcattgacct ttgtctgcct ggcatttctg atagggccga gcattaagaa ctccagcccg
    60961 gaagagatgg tgtcggtata ccatttctgg acgattgtgc tggcgattgc cggaatggtg
    61021 ctttacttca tctgcttcaa atcgacgcgt gagaatgtgg tacgtatcgt ggcgcagccg
    61081 tcattgaaga tcagtctgca aaccctgaaa cggaatcgcc cgctgtttat gttgtgcatc
    61141 ggtgcgctgt gtgtgctgat ttcgaccttc gcggtcagcg cctcgtcgtt gttctacgtg
    61201 cgctatgtgt taaatgatac cgggctgttc actgtgctgg tactggtgca aaacctggtt
    61261 ggtactgtgg catcggcacc gttggtgccg gggatggtcg cgaggatcgg taaaaagaat
    61321 accttcctga ttggcgcttt gctgggaacc tgcggttatc tgctgttctt ctgggtttcc
    61381 gtctggtcgc tgccggtggc gttggttgcg ttagccattg cctcaattgg tcagggcgtt
    61441 accatgaccg tgatgtgggc gctggaagct gataccgtag aatacggtga atacctgacc
    61501 ggcgtgcgaa ttgaagggct cacctattca ctattctcat ttacccgtaa atgcggtcag
    61561 gcaatcggtg gttcaattcc tgcctttatt ttgggattaa gcggatatat cgccaatcag
    61621 gtgcaaacgc cggaagtaat tatgggcatc cgcacatcaa ttgccttagt accttgcgga
    61681 tttatgctac tggcattcgt tattatctgg ttttatccgc tcacggataa aaaattcaaa
    61741 gaaatcgtgg ttgaaattga taatcgtaaa aaagtgcagc agcaattaat cagcgatatc
    61801 actaattaat attcaataaa aataatcaga acatcaaagg tgcaactatg agaaaaatag
    61861 tggccatggc cgttatttgc ctgacggctg cctctggcct tacctctgct tacgcggcgc
    61921 aactggctga cgatgaagcg ggactacgca tcagactgaa aaacgaattg cgcagggctg
    61981 ataagcccag tgctggcgcg ggaagagata tttacgcatg ggtacaggga ggattgctcg
    62041 atttcaatag tggttattat tccaatatta ttggcgttga aggcggagcg tattatgttt
    62101 ataaattagg tgctcgtgct gatatgagta cccggtggta tcttgatggt gataaaagtt
    62161 ttggctttgc cctcggggca gtaaaaataa aacccagtga aaatagcctg cttaaattag
    62221 gtcgcttcgg gacggattat agttatggta gcttacctta tcgtattccg ttaatggctg
    62281 gcagttcgca acgtacatta ccgacagttt ctgaaggagc attaggttat tgggctttaa
    62341 cgccaaatat tgatctgtgg ggaatgtggc gttcacgagt atttttatgg actgattcaa
    62401 caaccggtat tcgtgatgaa ggggtgtata acagccagac gggaaaatac gataaacatc
    62461 gcgctcgttc ttttttagcc gccagttggc atgatgatac cagtcgctat tctctggggg
    62521 catcggtaca gaaagatgtt tccaatcaga tacaaagtat tctcgagaaa agcataccgc
    62581 tcgacccgaa ttatacgttg aaaggggagt tgctcggctt ttacgcgcaa ctcgaaggtt
    62641 taagtcgtaa taccagccag cccaatgaaa cggcgttggt tagtggacaa ttgacctgga
    62701 atgcgccgtg gggaagcgta tttggcagtg gtggttattt gcgccatgca atgaatggtg
    62761 ccgtggtgga taccgacatt ggctatccct tttcattaag tcttgatcgt aaccgtgaag
    62821 gaatgcagtc ctggcaattg ggcgtcaact atcgtttaac tcccaattta cactgacatt
    62881 tgcaccgatt gtgactcgcg gctatgaatc cagtaaacga gatgtgcgga ttgaaggcgc
    62941 gggtatctta ggtggtatga actatcgggt cagcgaaggg ccgttacaag ggatgaattt
    63001 ctttcttgct gccgataaag ggcgggaaaa gcgcgatggc agtacgctgg gcgatcgcct
    63061 gaattactgg gatgtgaaaa tgagtattca gtatgacttt atgctgaagt aaaaaataac
    63121 gccggagaga aaaatctccg gcgttttggc tgtgaattac tgctgcggaa tagcgggtac
    63181 gaccggaaca tttaatgccg gcattgcaaa cataccaacg aaatcttcca gtggcatttt
    63241 ctgcccgttt aacgttatct gaccgttagt atattgcagg ctggtggtga tggtattgtc
    63301 ctgcaaggtg gtcagacgga acatctgccc cattgctgat gcaccttcaa cttgctgttt
    63361 cgccagtttt ttcgcttgat cttcctgata accttccagc ttcgctacct gagtcatcaa
    63421 ctcagttgcc atatccaccg gaatggtcag tttcgcatcc agagatttaa ccgaacgatc
    63481 tacttcctgc gccagcgttt gcggcgcttc tttagtcgtt gccggatctt tcaggaacag
    63541 cgacagattc agcgcacttt caccctgact gtttttccag cttagcggcg cgatagtaat
    63601 caccggatcg cctttcaaca tcagcggcag ggcgctaaag aaggcttccg tcactttctc
    63661 ctgataaagt tcgggattgt tggcaatttc tggctgtgcc agcagcgcct gagtttgcgc
    63721 gttatattgc tggctaaact gatgccaggc ttcgccatca atttggccga cttttaaagt
    63781 cagcttgccg ctgcccagat cctgattctg taccttcagg ctgtttagcg agtaatccag
    63841 ttggctattg atcgttttac cgtcattgac cagatccgat ttaccgctga tctccatgcc
    63901 ttccagcagt gccagttctt tgccttccac tgaaatggtc atttttacca gtgacagttt
    63961 ttggtttcct acgcgctcac caaaacttgc cagcgtgctg gaaccgtcgg ttttcagatt
    64021 attaaaggtc aactgtactt tctggttgta ttcgttaacc gcgtctatcc gaccactttg
    64081 cgcctccccg gaaagggaga tagctttgcc gtctctgtcc gcatttaact ggaactcgcc
    64141 gccgctaaag gcgacttttt catccttttg ctcgtaattt agtggcttga gcgaaatatc
    64201 ggaactggaa tcaccgctgt aaccaatgcg cgagttaatc tcaaaaggcg tttcaccttt
    64261 tgccatatca aacagtggtt tgcttacttc gttattaacc agcgtggttt gaattgatgc
    64321 catcgacggg atcaggttca gttttttaag ctgggcaagg gggaagggac catgatcaac
    64381 cgattcgttg aagatgacgc tctgaccgct tttaatccac ggattttctt ttccggcaat
    64441 gggtttcacc aacagttgca gctgactgct gaatacgccg cgatgatagt tttgataact
    64501 cacttccagg ttggattcag gagcggtcag tttgagttgc gcgttcgcct gcgcgaccat
    64561 gtcttcgaga tgggtttcaa tcttcttgcc tgtataccat gcgccgcctg tccagactac
    64621 gcctagcgca acaatgacgc ctaccgctac cagcgattta ttcataatga ttatccataa
    64681 aatgaaatca ggcggactgg ccgcctgaag gtgttataag cctttaataa gcttagcaag
    64741 agatgttaat tttttcagta agctcttaca gcttgttgta aacccgtgct aaacggccgt
    64801 ggcctttgac agtcaccggt gattcgttgg cggcaataaa cgctgattca cccggtttaa
    64861 gctgtaactg ctgagaacct ttccacaacg ttgcatcgcc ttcgacgcag aacaaaatgg
    64921 cggcactctg ctggctaatg gtggtttctt tatcactaag gtcatgcagc gagaaggcaa
    64981 aatcatcaac aggaatcggg aagtccagtt ctgcaccttg tttcaccggc tgggtcaaca
    65041 actggttagc cggtttggct tcgaacttca cattggcaac cagttccgga atatcaatgt
    65101 atttaggcgt cagacccgca cgcagcacgt tatcggagtt tgccatcact tccagcgcca
    65161 cgccttgcag gtaagcgtgc ggtgtttcag cgaacaggaa catcgcttcg ccaggattta
    65221 atttcaccac attcagcaac agcggagaga acaggccgct gtcatccggg taaaattcag
    65281 aaattaaacg aatcgtttgc cacggttcac cctgctgact gtcgagcgcc gattttaaaa
    65341 tcgccagcgc gcgggatttt tcttcaccct gcatattcaa caggctggcg aacagttcgc
    65401 ttaaacgttc ggcatcaggc tgttgtaaaa agtgagcaat cgccggatgt gcacctgcga
    65461 ccggctggag tagggagaca atctcggaaa attcacgaaa cgcgttcatc gcaaggaaag
    65521 gcgtcagcgc aaaaaccagc tccggcttgt ggttaggatc tttatagtta cgctcggcgg
    65581 catccatcgg gatacctgcg gcattttctt tggcaaaacc gatttcagac ttgtgtttgt
    65641 ttggatgaac ctgaatggag agtggctgtg ctgcgcataa tactttgaac aggaaaggca
    65701 gttcgccaaa gcgtttggca acggcctctc cgagcagagt cgatttatca ctctcaatca
    65761 catcacgcag tgaaacgata tctccggcgg cattctgcac tcgtgaactg cttttcggat
    65821 gtgcgcccat ccacagctcg gccatcggct ggctggacgg attttccata ccataaagtt
    65881 cagtcaacgc cgttttgctg ccccaggcat agttttgcac tgagttaatg agtttttgca
    65941 tgatcaatcc ctgttttaat gtggaaatta atcccgctat taaagcaaga atcctacggg
    66001 aagtaacctg gagccgcaaa aagtcgtact agtctcagtt tttgttaaaa aagtgcgtag
    66061 gatattgtta ctcgctttta acagggcaac ggaacacccg cccagagcat aaccaaacca
    66121 ggcagtaagt gagaaaacaa tgtcaaacaa accctttcat tatcaggctc cttttccact
    66181 caaaaaagat gatactgagt attacctgct aaccagcgaa cacgttagct tatctgaatt
    66241 tgaagggcag gagattttga aagtcgcacc cgaagcgtta actctgttgg cgcgccaggc
    66301 gtttcatgat gcgtcgttca tgctgcgtcc ggcgcaccaa caacaggtgg ccgacattct
    66361 gcgtgacccg gaggccagcg aaaatgataa atatgtggcg ctgcaattcc tgcgtaactc
    66421 cgacatcgcg gcgaaaggcg ttctgccaac ctgtcaggat accggcaccg cgattattgt
    66481 tggtaaaaaa gggcagcgtg tatggaccgg tggtggtgat gaagcggcgc tggcgcgcgg
    66541 tgtctataac acttatatcg aagataatct gcgctactcg caaaacgcgc cgctggatat
    66601 gtataaagaa gtgaataccg gcaccaatct gccagcgcag atcgatcttt atgccgttga
    66661 tggcgacgaa tacaaattcc tctgtatcgc caaaggtggt ggttcggcaa acaagacgta
    66721 tctctatcag gaaaccaaag cgttactgac gccggggaaa ctgaaaaatt acctggttga
    66781 gaagatgcgc acgctgggta cggcggcctg tcctccgtat catattgcgt tcgttattgg
    66841 tggaacttct gcagaaacga accttaaaac ggtgaaactg gcttccgcga aatactatga
    66901 tgaactgcca acggaaggga atgagcacgg tcaggcgttc cgcgatgtgg aattggaaaa
    66961 agaattgctg atcgaagcgc aaaatcttgg tctgggtgcg cagtttggcg gtaaatactt
    67021 cgctcacgac atccgcgtga ttcgcctgcc acgtcacggc gcatcctgcc cggtcggtat
    67081 gggcgtctcc tgttctgctg accgtaatat caaagcgaag atcaaccgtc aggggatctg
    67141 gatcgaaaaa ctggaacata atccaggcaa atatatcccg gaagagctgc gcaaagcggg
    67201 agaaggcgaa gcggtgcgcg ttgaccttaa ccgtccgatg aaagagatcc tcgcacagtt
    67261 gtcgcagtat cccgtttcta cacgcttatc gcttaacggc acgattatcg tcggtcgtga
    67321 tattgctcac gccaaactga aagagcggat ggataacggt gaagggctgc cgcagtacat
    67381 caaagatcat ccgatttact acgcgggtcc ggccaaaacg ccggaaggtt atgcctccgg
    67441 ttctcttggc ccaacgaccg ccggacggat ggattcttat gtcgatcaac tgcaagcgct
    67501 gggcggaagt atgatcatgc tggcgaaagg caaccgcagc cagcaggtga cggatgcctg
    67561 taaaaaacac ggtggcttct accttggcag tatcggtggt ccggccgctg tattggcgca
    67621 gggaagtatt aagagcctgg aatgtgttga atatccggaa ctgggaatgg aagccatctg
    67681 gaaaattgaa gtggaagatt tcccggcgtt tatccttgtg gatgataaag gaaatgactt
    67741 cttccagcag atacaactca cacaatgcac ccgctgtgtg aaataaacag agccgccctt
    67801 cggggcggtt tttttacatg gcacgaaaga ccaaacattt gttatcaaat agtaaataat
    67861 gagtgagcta aaagttactt aacgaaagca aaacagaaag aaaaaattaa tcaggtgagg
    67921 agtaggccat gaatacagta cgcagcgaaa aagattcgat gggggcgatt gatgtcccgg
    67981 cagataagct gtggggcgca caaactcaac gctcgctgga gcatttccgc atttcgacgg
    68041 agaaaatgcc cacctcactg attcatgcgc tggcgctaac caagcgcgca gcggcaaaag
    68101 ttaatgaaga tttaggcttg ttgtctgaag agaaagcgag cgccattcgg caggcggcgg
    68161 atgaagtact ggcaggacag catgacgacg aattcccgct ggctatctgg cagaccggct
    68221 ccggcacgca aagtaacatg aacatgaatg aagtgctggc taaccgggcc agtgaattac
    68281 tcggcggcgt gcgcgggatg gaacgtaaag ttcaccctaa cgacgacgtg aacaaaagcc
    68341 aaagttccaa cgatgtcttt ccgacggcga tgcacgttgc ggcgctgctg gcgctgcgca
    68401 agcaactcat tccgcagctt aaaaccctga cacagacgct gagtgaaaaa tcgcgtgcat
    68461 ttgccgatat cgtaaaaatc ggtcgaaccc acttgcagga cgccaccccg ctaacactgg
    68521 ggcaggagat ttccggctgg gtagcgatgc tcgagcataa tctcaaacat atcgaataca
    68581 gcctgcctca cgtagcggaa ctggctcttg gcggtacagc ggtgggtact ggactaaata
    68641 cccatccgga gtatgcgcgt cgcgtagcag atgaactggc agtcattacc tgtgcaccgt
    68701 ttgttaccgc gccgaacaaa tttgaagcgc tggcgacctg tgatgccctg gttcaggcgc
    68761 acggcgcgtt gaaagggttg gctgcgtcac tgatgaaaat cgccaatgat gtccgctggc
    68821 tggcctctgg cccgcgctgc ggaattggtg aaatctcaat cccggaaaat gagccgggca
    68881 gctcaatcat gccggggaaa gtgaacccaa cacagtgtga ggcattaacc atgctctgct
    68941 gtcaggtgat ggggaacgac gtggcgatca acatgggggg cgcttccggt aactttgaac
    69001 tgaacgtctt ccgtccaatg gtgatccaca atttcctgca atcggtgcgc ttgctggcag
    69061 atggcatgga aagttttaac aaacactgcg cagtgggtat tgcaccgaat cgtgagcgaa
    69121 tcaatcaatt actcaatgaa tcgctgatgc tggtgactgc gcttaacacc cacattggtt
    69181 atgacaaagc cgcggagatc gccaaaaaag cgcataaaga agggctgacc ttaaaagctg
    69241 cggcccttgc gctggggtat cttagcgaag ccgagtttga cagctgggta cggccagaac
    69301 agatggtcgg cagtatgaaa gccgggcgtt aatctgcaac atacaggtgc agccgtggaa
    69361 tgatcaaacg aagcggctgc gcctgaggtt tataacggtg ctgcacattg tcggcatcgt
    69421 aatttaacaa ctcaccaacg tccggcacgc ccgcaccatt atcccgatta atcagtgcaa
    69481 tcagcggtgt agggcaggcg tgttggactt gtttttgatc tcctttgtac cagacgcggg
    69541 caatcggctg caccttcacc ggacgtttga tttttaactt cgcattctgt ggcagggcag
    69601 cgatatcctg atactctcgc tccagttttc tttgccactc ctcgcgcgtc cacggtgcga
    69661 cactgcgtgg tgatttcagg cttttttcca gctgtgccag gacttcatca cgatgcaaat
    69721 tcttaatgat atgtttatta gcccaaccaa agcgtaaagt ggcgggatcg tgcagaacgg
    69781 tgagcgtgcg gtaagcatta agggtgatca gccccggcaa atgacgatgc acccattcaa
    69841 aacgtgccgc ggtggggagt tctgattcaa ccgtgacgat atgctcgaac gtggtcttga
    69901 gtttattgat gtgctgaata tgactgacca acgctgcttg cgaaaggtta tcgacctggt
    69961 aacacaacac gccaggcaga cggacagctg ctttgctgct gcgattttcg gactgttgtt
    70021 gaataaataa atgacggaaa tgacgcaacg cctgcgactg cgcgtcgttg ccgagatgtt
    70081 gtttcacctc aatacgatta agcggattat gctcatcctc tttttttacc tccggcaaag
    70141 agaacacgcg ggcaaccaat agcttgtgtt gctcaagatg agcggcaaat gcagccagct
    70201 cttgttccat ctggcgaaaa gtagtgttga gtcggtctac gagatcgtaa cgcgccataa
    70261 tattaaccac tttagttaca acatccttaa tttatagcac agtggtggtg acttgaccac
    70321 cgtgcgcgta gtgtcaagca gaggtaaatt gcgggatgtt atgccataac ggccagctaa
    70381 acgagaagcg ggcaccaccc agttcgctgg tgtcacaatt aaccgtaccg cccattgcca
    70441 gtgctataga gtggacaatt gccagcccca gcccgcagcc gccggttgag cgatcccggc
    70501 tgggatcgag gcgaacaaaa ggttcaaaga tatgttcgcg gttttctggg gcaatccctg
    70561 ggccatcatc ctcaacaatt aatgtcgctc tattccccga cagtagcagg ctggtttcaa
    70621 ccgttgaatg gcagtagcgc agggcattat tgagcaaatt atccagcacg cgctccatta
    70681 agcgcatatc caacgccgca taatggcctt gcatgagcgt ttttatccgt accgttttat
    70741 cgggcgttac tgcctgaata tctgccagat gcgttgacag ccacaacggc aggtctggtt
    70801 cgctaagatg aagctcgttt tgtgggcgat cgagtcgggc ataagtcagc agctcttcaa
    70861 ttaaagcttc aagttgactg atatcacgat tcaacgcctg ggattcggcg gcgctcaggt
    70921 tatcgctcat ctccagtcga taacgcaggc gcactaacgg tgttcgcagt tcgtgagcga
    70981 taccgtcaat aagctgtttt ttgctggcaa tcaaggcgtt gatattgtcc gccatctggt
    71041 taaatgcgat accaagtcgt tcaaagctcg aaccctcatc aaagtggata cgttcattga
    71101 gatgcccatc gccaaatcgt tgcgccgctg cttccagttt taacatatcc tgccagtgcg
    71161 gacgcatcca gataaacacc ggaaaggcga gggaaatagc aataaaagcg atcagggcga
    71221 tatccagcaa tcgcatctga tggagataat aaagataagg aacaggacca actgccagca
    71281 cgtagtggct gcgcgggata cgctgcaaaa aagtgtactg atcgtccagg gcgacaattt
    71341 cgccgccacg cagccggtgc atggaaatat catcaagatg gtatttactc agtggctcga
    71401 ctcgcagatc gaaagagaga tttaaatcca tctctttcag cgtcttaccc cagtcatgtg
    71461 gggggatctc acgcaattcg ctgcgcatca gatacagcga actgttcatc aaatcatcca
    71521 gcgactgttt gcccgcgcgt tcggcggtaa atttgtacac caacccaacc agcagagaca
    71581 tcacaaggaa gcagacaaac aataacaggt aaaactggat aaacagtttt ttcatcgctt
    71641 attcccatgc atgaggcgca aaaagatagc ctttgttacg cacagtttta atgcgataag
    71701 gttcagcggc gttatcgagc agtttttttc ttaaccgcga aatagccacg tccacgctac
    71761 gatccaggcc gtcataactg acgccgcgta aatttttcag caatgcatcg cggtccatga
    71821 tttgcccggc atgggtagct aattcccaca ataattcgaa atcagctgtc gagagcgaga
    71881 tttcagtgtt agccagggtg actacgcggt tgataggatc gatggtcaac gtgccgaaat
    71941 gcagggcttt gtagggagtc agtgacgttt cctgaagacc tttggtcagc gtggcttgct
    72001 cattctgacg caaatgcaaa cgtaaacgcg ctaacaaaac agcagggggc gtcgttttga
    72061 gaatatagtc gcaggcaccc atttccagtg ccaggatgtg gttcatatcg ctatcgagag
    72121 aggttagaag aacaatcggt ccggaccact ttgcgcgtaa atcacgacaa atggtcatgc
    72181 cgtccttgcc tggtagcatg atgtcgagta acaccaaatc cggattttct cgcaaaatgg
    72241 tttcttcggc ctggtcgccg cgcggctcta cggtaacctg tatatcatgt tttgccaggt
    72301 acgcggcaat cagtgaaccg acttccgcat catcttccac aaatacgata gtgttcataa
    72361 cattcacggt agatataaaa acgtcacaat acaccgcgct gtttttactt actatcaatc
    72421 gtttctaaac aagttttcat caggtattct actgatgact tgttatggaa gtgttaaggt
    72481 aaaaagatgg ggctggtaat caaagccgcg ctcggtgcgc tggtagtgct gttgattggt
    72541 gttttagcaa aaacgaaaaa ttattatatc gccgggctga ttccactttt cccgaccttt
    72601 gcgcttatcg cgcattatat tgttgccagc gaacgcggca ttgaagcctt acgcgcaacc
    72661 atcattttta gtatgtggtc gattattccc tattttgttt acctggtgtc gctgtggtat
    72721 ttcaccggaa tgatgcgctt gcccgccgct tttgttggtt ctgttgcgtg ttgggggata
    72781 agcgcatggg tgttgattat atgctggata aagctgcatt aacgcagatg acgaccgcca
    72841 tcaagtggga aactgcgtcc ggtgacaaag caactggtaa gtaagtaatc gaccaggtcg
    72901 atcacttctt tctcgccagg cgcggttttc atcaatgatt tattcagcgc ctgttgtcga
    72961 tattcggcat catcatgttc attaaacagg atcagcgatg gcgcaataga attcactttc
    73021 acttccggtg ccagcttgcg ggcaaacgag cgggtcatat tatccagtgc cgctttgctt
    73081 gcagcatacg caatatgttt gtcgctaccg cgctccacca cataatcggt aaagtgaatg
    73141 atatcgctgg cggcgtgtcc gtgcccacgc agtaatcttt ccagcgcatg gttgagcagg
    73201 tatggggtat taacgtggat ctgcatcatg caagccagta cgtcggccag tggcgcaccc
    73261 ggtttttccg ccatccacgc actggcgtta tgcaaaatag cacgcagacc atgggtgctt
    73321 tttagtactt catcggcaaa cgccatcaca ccgtcgttgg tcgaaaaatc agcctgaata
    73381 cactgcgcac ctgcattaat cagtccatca atggctggat agtgtgtccg atagctgaca
    73441 atcaccggtt gcttttgatt aatgaaatgc catgcgaggg cgaggccgat gcgacgacct
    73501 ccgccagtaa ttaatattgg caagggctgg gttttaccca tcgttatctc ctttgctatc
    73561 caacgatggg atgaacgtta tcccaccagc atccaggtcg ccggaactga ggcaattaac
    73621 agcatgccaa tcagcaccat ttcctgacgg ttcagtacat tatcatgtgt atgcgttttg
    73681 cgcgcataca ggaaaaccag cagccccggt gcatacagaa caacggataa cagcaagtgc
    73741 attggaccag aggcgtacaa taaccataag ccataaatgc aggcaccgac acctaccgct
    73801 ttatgcagtg gacgggttgc gattttcagc aagaacgcgc cgaccaggaa atagggcacc
    73861 agaatcattt ctgaggcgat ggtcagcaac gtgttgtaat cggaaccggt cagccagatg
    73921 agtaccagac aaatttgcac gcagatgttt gtcaaccaca gtgaggcgga tggcgcagct
    73981 tgtgcgttct ggcgcgcaaa aatgcgtggg aatgctttat gagtggcagc caggaacgga
    74041 acttctgccg ccatgatggt ccagcttaag tacgcgccgc aaacggaaac gatcaaaccg
    74101 gcagcgatga tgatttcgcc ccacggtccc atcatttcca ccatcagacc ggccattgac
    74161 gggttacgaa tttcagccag ttcaggacgt gccaccactc ccagcgaaag cagcgttacc
    74221 agcaagtaaa cgcccagagc ggagagaacc gccagcagtg tcgctttgcc aacatcacgt
    74281 ttattacgcg cacgcgcaga aacaaccacg gcaccttcca caccaatgaa aacccacagg
    74341 gtgatcagca tggtgttttt cacctgttcc caaacaggta cgccaagtgc aagtccggtg
    74401 aagtcgagct tgaagatatc cagtttgaac atcatcatcg ccagcacaac aaacagaccc
    74461 agcggcaaca attttgccag tgtcgccacc aggttaatgc tggcagcggt ttgcaccccg
    74521 cgcaggatca aaaagtgaac aatccacaat aaagccgatg caccgactat cgattgccag
    74581 gtattaccat cgccaaacag gcgcaattcc ggcgtgtccg taaaaaagct taacgcggaa
    74641 aaaacgatca ccagatagga gacgttggcg atgactgcgc acagccagta tccccatgcg
    74701 gaacaaaagc cgattagctc gccaaaccct tcgcgggcat aggtaaagat accgccgtca
    74761 agttcgggac gaatgcgcgt gaggatcagc atggcaaagg ccagcaataa aatgccagcg
    74821 ccagtaatac cccagccgat gagcagtgct gccgggctgg caactgccgc catattttgc
    74881 ggcagactga aaacacccgc gcccagcatt gagcttaata ccagcgcggt gagtgcgctc
    74941 agtcccagtt tcttttccat cgatatccag tgattaaaac gtgataagaa gaataaatat
    75001 tagggcaaaa cgcataaaaa tggagtttgc cactgaagcg cgggatttta cgaagagatg
    75061 tgtctgcatg caatggtcgc ggggaaattt gtgactaaaa atgcgaaagg cggcatcaag
    75121 ccgccttata agagtaactg aatagttatt tatacagatc tgcactgacg gtcaggttat
    75181 taccacgttc ctgccactgg cgggtgatgt ggtaatattt agcacctttc ttcgcggctc
    75241 gtttcgcaac ctgataggag acttcggtca tgttgccgta gttgccagag aatttgatgc
    75301 tgtcgaacgg gaccatcatc gctgctgtcg ctttgttcag ttcttcgact ttagtgccat
    75361 ccggaagcgt gacagtgtaa cgcccgcctt ttgatgactg ggtttcaaag aagcgaccga
    75421 cttcagaact tggtgatgcg gtagtcgcaa cacccgggat ctcaactttc ttcgcggctt
    75481 cgccaccggc agccagagct gcacgtcctg cttcggaatc tgccgggatc acatccgggc
    75541 tctggacgat acgtttctta gcatcttttt tatagatgaa tgcagtaata cgctggttgc
    75601 cgccctggtt ggcatcgatt tgacgaacga tgtagaaaga gtaggcacct ttcgcttttg
    75661 ccgctttggt gatggcatca ttgacttctg gctggctacg atagaagccc tggacggtga
    75721 ccgtgtcaaa cggttcaatc agaacagcct gatctttcgg cagttcgaca acaccgttaa
    75781 ttacgcgatt acttgtttct tctgcttttt cagcatcggc tttatagagg tcagcgacca
    75841 cacgccagtt accgctgttc ccaaaatcag aagtgtcgac aacataaaaa gaggcggcac
    75901 cttctttatc tgcgcgacga gaaacggctt tcaccgcttc gccaatagca ttaaaacgac
    75961 cggtaaccac tacacggtca aaaggtttaa ccgctgccgc ttgctccggt gtcagttctg
    76021 ttgctgcgtt aacggagaat gccgtagcag aaagcagtgc cgacgccagg agggtgttct
    76081 taagcttcat aaaaataatc cttcgccttg cgcaaaccag gtactggtat tgttattaac
    76141 gagaaacgtg gctgattatt gcatttaaac ggtgtaactg tctgcgtcat ttttcatatc
    76201 acattcctta ag
//
LOCUS       DBFIFI010000004        66134 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.004, whole genome shotgun sequence.
ACCESSION   DBFIFI010000004 DBFIFI010000000
VERSION     DBFIFI010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 66134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66134
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.004"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..761)
                     /locus_tag="N7B39_000230"
     CDS             complement(<1..761)
                     /locus_tag="N7B39_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HGU2420287.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTKCHGESLRGHIDVTTEDLASALDVTEDVIDSILHAMQGRVLDGDLISGWEKRQV
                     MKEDNGNVSQTAKSPAERKRAQRERERLREQNTDCHDESRRVTHMSRQVTTDTDTDKE
                     LNPTHNARMRESAPASESNGAPLQTAEPEYPDGLSEPIGKFPMTGVWRPSLDFRQRAA
                     LWGMALPEPEFTPAELAAFRDYWMAEGKVFTQVQW"
     gene            complement(830..1255)
                     /locus_tag="N7B39_000231"
     CDS             complement(830..1255)
                     /locus_tag="N7B39_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HGU2420288.1"
                     /translation="MKIKHEHIRMAMNAWAYPDGEKVPAAEIARTYFELGMTFPELYD
                     DSHPEALARNTQKIFRWLDKDTPDAVEKMQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVAAAIAWGTLTNSGGQPGNAVVVH"
     gene            complement(1239..1562)
                     /locus_tag="N7B39_000232"
     CDS             complement(1239..1562)
                     /locus_tag="N7B39_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU2420289.1"
                     /translation="MKKDNYSFKRACAVVGGQSAMARLLGVSPPSVNQWIKGVRQLPA
                     ERCPAIERATKGGVLCEELRPDVDWTYLRRSSCYSQNMSMKQPNDENDHTRSIKRQMI
                     HENQT"
     gene            1687..2163
                     /gene="racR"
                     /locus_tag="N7B39_000233"
                     /gene_synonym="rarC"
     CDS             1687..2163
                     /gene="racR"
                     /locus_tag="N7B39_000233"
                     /gene_synonym="rarC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor RacR"
                     /protein_id="HGU2420290.1"
                     /translation="MLSGKDLGRAIEQAINKKIASGAVKSKAEIARHFKVQPPSIHDW
                     IKKGSISKDKLPELWRFFSDVVGPEHWGLNEYPIPTPSTSDTKSELLDINSLYQAASD
                     EKRAIVAFLLSGNATEPSWVDHDVRAYIAAMEMKVANYLKNQESKRKSQNITKTGT"
     gene            2479..2631
                     /locus_tag="N7B39_000234"
     CDS             2479..2631
                     /locus_tag="N7B39_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HGU2420291.1"
                     /translation="MDTLDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTESGARRW
                     LARNSD"
     gene            complement(2746..3261)
                     /locus_tag="N7B39_000235"
     CDS             complement(2746..3261)
                     /locus_tag="N7B39_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2420292.1"
                     /translation="MIAELTAAMTAIRETAQIAKLMNEAKTQAEVNAAIGELNSKLAS
                     IQRECVSLVELVGTYQEINASLKAKIAEFENFEAQTEGYILSQLESGTFVYSKEVTVN
                     GGSIIMHLCPKCFGQKIVSILQPFPVREYEFFHKSRCLYCENQFLMNKNPDYVSPPSI
                     EELARKLNGNL"
     gene            3385..3783
                     /locus_tag="N7B39_000236"
                     /pseudo
     CDS             3385..3783
                     /locus_tag="N7B39_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
     gene            3845..4114
                     /locus_tag="N7B39_000237"
     CDS             3845..4114
                     /locus_tag="N7B39_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HGU2420293.1"
                     /translation="MSEQYLITLDEWKPKRFSLPITNTTLVKYGKLGYIVPRPQKIRG
                     RWLIDRRAVFVGPGETGIAPEIHTGDDDALKEILTHVTEATKKQH"
     gene            4083..5201
                     /locus_tag="N7B39_000238"
     CDS             4083..5201
                     /locus_tag="N7B39_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HGU2420294.1"
                     /translation="MSPRPRKNSTDVAGLYEKFDRRTGRVYYQYKNPVTGKFHGLGTD
                     KGKAEKIASTANQRIAAAEAEYFMRKIDESPSATKRRGIRLKAWVDRYLKIQDTRLKN
                     GDIAATTHKEKTRMAAYLVSRLGNHPLKELEVRDFALILDEWLDKDMVSTARVNRGLW
                     VDIYKEAQHAGEVPPGWNPPEATRKPIPKVTRARLTMEDWQKIYNATPEKHFIRNAML
                     LAIVTGQRRDDICHMRFSDVWNEHLHITQGKTGMRLALPLTLRCDAIGITLKEVIDGC
                     RDRILSPYLIHSRHQKQPKPMSKDNLSDYFAKARDLAGVIPPAGKTPPTFHEQRSLSE
                     RLYRAQGIDTKTLLGHKVQATTDRYNDTRGQEWVKLVI"
     gene            5368..6162
                     /gene="potC"
                     /locus_tag="N7B39_000239"
     CDS             5368..6162
                     /gene="potC"
                     /locus_tag="N7B39_000239"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HGU2420295.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTIAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            6159..7205
                     /gene="potD"
                     /locus_tag="N7B39_000240"
     CDS             6159..7205
                     /gene="potD"
                     /locus_tag="N7B39_000240"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HGU2420296.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLSPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(7361..8182)
                     /gene="cobB"
                     /locus_tag="N7B39_000241"
     CDS             complement(7361..8182)
                     /gene="cobB"
                     /locus_tag="N7B39_000241"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HGU2420297.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(8198..9109)
                     /gene="nagK"
                     /locus_tag="N7B39_000242"
     CDS             complement(8198..9109)
                     /gene="nagK"
                     /locus_tag="N7B39_000242"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HGU2420298.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLPAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(9138..10382)
                     /gene="lolE"
                     /locus_tag="N7B39_000243"
     CDS             complement(9138..10382)
                     /gene="lolE"
                     /locus_tag="N7B39_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HGU2420299.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(10382..11083)
                     /gene="lolD"
                     /locus_tag="N7B39_000244"
     CDS             complement(10382..11083)
                     /gene="lolD"
                     /locus_tag="N7B39_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HGU2420300.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPWLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(11076..12275)
                     /gene="lolC"
                     /locus_tag="N7B39_000245"
     CDS             complement(11076..12275)
                     /gene="lolC"
                     /locus_tag="N7B39_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HGU2420301.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            12537..13610
                     /gene="ycfT"
                     /locus_tag="N7B39_000246"
     CDS             12537..13610
                     /gene="ycfT"
                     /locus_tag="N7B39_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HGU2420302.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLAALAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPVAVSYSR"
     gene            13738..17184
                     /gene="mfd"
                     /locus_tag="N7B39_000247"
     CDS             13738..17184
                     /gene="mfd"
                     /locus_tag="N7B39_000247"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HGU2420303.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMEKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLENSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLESFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDTVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     LSRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            17328..18290
                     /gene="ldtC"
                     /locus_tag="N7B39_000248"
     CDS             17328..18290
                     /gene="ldtC"
                     /locus_tag="N7B39_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HGU2420304.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(18373..18630)
                     /gene="bhsA"
                     /locus_tag="N7B39_000249"
                     /gene_synonym="ycfR"
     CDS             complement(18373..18630)
                     /gene="bhsA"
                     /locus_tag="N7B39_000249"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HGU2420305.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            18871..19503
                     /gene="comR"
                     /locus_tag="N7B39_000250"
     CDS             18871..19503
                     /gene="comR"
                     /locus_tag="N7B39_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HGU2420306.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTGKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(19565..20104)
                     /gene="ycfJ"
                     /locus_tag="N7B39_000251"
     CDS             complement(19565..20104)
                     /gene="ycfJ"
                     /locus_tag="N7B39_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HGU2420307.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDEHRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(20331..21635)
                     /gene="ndh"
                     /locus_tag="N7B39_000252"
     CDS             complement(20331..21635)
                     /gene="ndh"
                     /locus_tag="N7B39_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HGU2420308.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDRDAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVELTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKLLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(22035..22577)
                     /gene="ycfP"
                     /locus_tag="N7B39_000253"
     CDS             complement(22035..22577)
                     /gene="ycfP"
                     /locus_tag="N7B39_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HGU2420309.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(22600..23625)
                     /gene="nagZ"
                     /locus_tag="N7B39_000254"
     CDS             complement(22600..23625)
                     /gene="nagZ"
                     /locus_tag="N7B39_000254"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HGU2420310.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPEKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSTLIRENKLDAIMPAHVIYSD
                     VDPRPASGSSYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQVSLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISARLNQLHERW
                     QEEKAGH"
     gene            complement(23636..24460)
                     /gene="thiK"
                     /locus_tag="N7B39_000255"
     CDS             complement(23636..24460)
                     /gene="thiK"
                     /locus_tag="N7B39_000255"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HGU2420311.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLHQYRALSQLPACIAPKPHLYLRDWMVVDYLPGEVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(24441..25082)
                     /gene="lpoB"
                     /locus_tag="N7B39_000256"
     CDS             complement(24441..25082)
                     /gene="lpoB"
                     /locus_tag="N7B39_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HGU2420312.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(25096..25473)
                     /gene="ycfL"
                     /locus_tag="N7B39_000257"
     CDS             complement(25096..25473)
                     /gene="ycfL"
                     /locus_tag="N7B39_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HGU2420313.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(25476..25835)
                     /gene="hinT"
                     /locus_tag="N7B39_000258"
     CDS             complement(25476..25835)
                     /gene="hinT"
                     /locus_tag="N7B39_000258"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HGU2420314.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            25966..26154
                     /locus_tag="N7B39_000259"
     CDS             25966..26154
                     /locus_tag="N7B39_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012138106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2420315.1"
                     /translation="MLPYISPLFPFLPANALKRHSLFGLHDKYECVYFSFAFAFAFAF
                     ASKIIRTLNITAHHPPPA"
     gene            26187..28376
                     /gene="fhuE"
                     /locus_tag="N7B39_000260"
     CDS             26187..28376
                     /gene="fhuE"
                     /locus_tag="N7B39_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HGU2420316.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(28436..29869)
                     /gene="ptsG"
                     /locus_tag="N7B39_000261"
     CDS             complement(28436..29869)
                     /gene="ptsG"
                     /locus_tag="N7B39_000261"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HGU2420317.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(30164..30961)
                     /gene="ycfH"
                     /locus_tag="N7B39_000262"
     CDS             complement(30164..30961)
                     /gene="ycfH"
                     /locus_tag="N7B39_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HGU2420318.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(30972..31976)
                     /gene="holB"
                     /locus_tag="N7B39_000263"
     CDS             complement(30972..31976)
                     /gene="holB"
                     /locus_tag="N7B39_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HGU2420319.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(31973..32614)
                     /gene="tmk"
                     /locus_tag="N7B39_000264"
     CDS             complement(31973..32614)
                     /gene="tmk"
                     /locus_tag="N7B39_000264"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HGU2420320.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEIITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTRWVKELDA"
     gene            complement(32604..33626)
                     /gene="yceG"
                     /locus_tag="N7B39_000265"
     CDS             complement(32604..33626)
                     /gene="yceG"
                     /locus_tag="N7B39_000265"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HGU2420321.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRYLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYITVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAIASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(33629..34438)
                     /gene="pabC"
                     /locus_tag="N7B39_000266"
     CDS             complement(33629..34438)
                     /gene="pabC"
                     /locus_tag="N7B39_000266"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HGU2420322.1"
                     /translation="MFLINGYKQESLSVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGITLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGGVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(34558..35799)
                     /gene="fabF"
                     /locus_tag="N7B39_000267"
     CDS             complement(34558..35799)
                     /gene="fabF"
                     /locus_tag="N7B39_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HGU2420323.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(35887..36123)
                     /gene="acpP"
                     /locus_tag="N7B39_000268"
     CDS             complement(35887..36123)
                     /gene="acpP"
                     /locus_tag="N7B39_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HGU2420324.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(36334..37068)
                     /gene="fabG"
                     /locus_tag="N7B39_000269"
     CDS             complement(36334..37068)
                     /gene="fabG"
                     /locus_tag="N7B39_000269"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HGU2420325.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILVQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(37081..38010)
                     /gene="fabD"
                     /locus_tag="N7B39_000270"
     CDS             complement(37081..38010)
                     /gene="fabD"
                     /locus_tag="N7B39_000270"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HGU2420326.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQHGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIGKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(38026..38979)
                     /gene="fabH"
                     /locus_tag="N7B39_000271"
     CDS             complement(38026..38979)
                     /gene="fabH"
                     /locus_tag="N7B39_000271"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HGU2420327.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(39047..40117)
                     /gene="plsX"
                     /locus_tag="N7B39_000272"
     CDS             complement(39047..40117)
                     /gene="plsX"
                     /locus_tag="N7B39_000272"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HGU2420328.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(40198..40371)
                     /gene="rpmF"
                     /locus_tag="N7B39_000273"
     CDS             complement(40198..40371)
                     /gene="rpmF"
                     /locus_tag="N7B39_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HGU2420329.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(40423..40944)
                     /gene="yceD"
                     /locus_tag="N7B39_000274"
     CDS             complement(40423..40944)
                     /gene="yceD"
                     /locus_tag="N7B39_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HGU2420330.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            41143..41727
                     /gene="yceF"
                     /locus_tag="N7B39_000275"
     CDS             41143..41727
                     /gene="yceF"
                     /locus_tag="N7B39_000275"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HGU2420331.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPHSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(41828..42787)
                     /gene="rluC"
                     /locus_tag="N7B39_000276"
     CDS             complement(41828..42787)
                     /gene="rluC"
                     /locus_tag="N7B39_000276"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HGU2420332.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDDGLKRCLQKLRNAR"
     gene            43360..46545
                     /gene="rne"
                     /locus_tag="N7B39_000277"
     CDS             43360..46545
                     /gene="rne"
                     /locus_tag="N7B39_000277"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HGU2420333.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPATPA
                     QPGLLSRFFGALKALFSGGEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETREGRQQAEVTEKARTADEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEETV
                     VAPVAEETVAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVTAVKPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(46740..47693)
                     /gene="flgL"
                     /locus_tag="N7B39_000278"
     CDS             complement(46740..47693)
                     /gene="flgL"
                     /locus_tag="N7B39_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HGU2420334.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDNDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEVNGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(47705..49348)
                     /gene="flgK"
                     /locus_tag="N7B39_000279"
     CDS             complement(47705..49348)
                     /gene="flgK"
                     /locus_tag="N7B39_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HGU2420335.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNSQHKAGFDANGDEGEDFFAIGKPA
                     VLQNTKNNGNVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(49414..50355)
                     /gene="flgJ"
                     /locus_tag="N7B39_000280"
     CDS             complement(49414..50355)
                     /gene="flgJ"
                     /locus_tag="N7B39_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HGU2420336.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTTGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(50355..51452)
                     /gene="flgI"
                     /locus_tag="N7B39_000281"
     CDS             complement(50355..51452)
                     /gene="flgI"
                     /locus_tag="N7B39_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309485.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HGU2420337.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAIA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(51464..52162)
                     /gene="flgH"
                     /locus_tag="N7B39_000282"
     CDS             complement(51464..52162)
                     /gene="flgH"
                     /locus_tag="N7B39_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HGU2420338.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(52215..52997)
                     /gene="flgG"
                     /locus_tag="N7B39_000283"
     CDS             complement(52215..52997)
                     /gene="flgG"
                     /locus_tag="N7B39_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HGU2420339.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(53135..53890)
                     /gene="flgF"
                     /locus_tag="N7B39_000284"
     CDS             complement(53135..53890)
                     /gene="flgF"
                     /locus_tag="N7B39_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HGU2420340.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADITPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(53910..55115)
                     /gene="flgE"
                     /locus_tag="N7B39_000285"
     CDS             complement(53910..55115)
                     /gene="flgE"
                     /locus_tag="N7B39_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HGU2420341.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPSVNAFDASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGAEPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(55140..55835)
                     /gene="flgD"
                     /locus_tag="N7B39_000286"
     CDS             complement(55140..55835)
                     /gene="flgD"
                     /locus_tag="N7B39_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HGU2420342.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNNGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(55847..56251)
                     /gene="flgC"
                     /locus_tag="N7B39_000287"
     CDS             complement(55847..56251)
                     /gene="flgC"
                     /locus_tag="N7B39_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HGU2420343.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(56255..56671)
                     /gene="flgB"
                     /locus_tag="N7B39_000288"
     CDS             complement(56255..56671)
                     /gene="flgB"
                     /locus_tag="N7B39_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HGU2420344.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRNATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            56826..57485
                     /gene="flgA"
                     /locus_tag="N7B39_000289"
     CDS             56826..57485
                     /gene="flgA"
                     /locus_tag="N7B39_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HGU2420345.1"
                     /translation="MLAIKRSVAIIAILFSPLSAASNLTSQLQTFFSSQLAGISDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLGAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            57561..57854
                     /gene="flgM"
                     /locus_tag="N7B39_000290"
     CDS             57561..57854
                     /gene="flgM"
                     /locus_tag="N7B39_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HGU2420346.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            57859..58275
                     /gene="flgN"
                     /locus_tag="N7B39_000291"
     CDS             57859..58275
                     /gene="flgN"
                     /locus_tag="N7B39_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HGU2420347.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQEIAVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGSKKISI"
     gene            complement(58315..59850)
                     /gene="murJ"
                     /locus_tag="N7B39_000292"
     CDS             complement(58315..59850)
                     /gene="murJ"
                     /locus_tag="N7B39_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HGU2420348.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGVLQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(59960..60883)
                     /gene="yceM"
                     /locus_tag="N7B39_000293"
     CDS             complement(59960..60883)
                     /gene="yceM"
                     /locus_tag="N7B39_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HGU2420349.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYCELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITEMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(60885..61532)
                     /gene="yceH"
                     /locus_tag="N7B39_000294"
     CDS             complement(60885..61532)
                     /gene="yceH"
                     /locus_tag="N7B39_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HGU2420350.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVELTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(61543..62127)
                     /gene="rimJ"
                     /locus_tag="N7B39_000295"
     CDS             complement(61543..62127)
                     /gene="rimJ"
                     /locus_tag="N7B39_000295"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HGU2420351.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            62363..63571
                     /gene="mdtH"
                     /locus_tag="N7B39_000296"
     CDS             62363..63571
                     /gene="mdtH"
                     /locus_tag="N7B39_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HGU2420352.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSVHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            63635..64282
                     /gene="grxB"
                     /locus_tag="N7B39_000297"
     CDS             63635..64282
                     /gene="grxB"
                     /locus_tag="N7B39_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HGU2420353.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            64416..64976
                     /gene="yceB"
                     /locus_tag="N7B39_000298"
     CDS             64416..64976
                     /gene="yceB"
                     /locus_tag="N7B39_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGU2420354.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVALTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            65082..66128
                     /gene="pyrC"
                     /locus_tag="N7B39_000299"
     CDS             65082..66128
                     /gene="pyrC"
                     /locus_tag="N7B39_000299"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HGU2420355.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILHAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSVDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEHQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
BASE COUNT        16175 a        17672 c        16456 g        15831 t
ORIGIN      
        1 tcccactgaa cctgcgtgaa aaccttcccc tccgccatcc agtaatcccg gaatgcggca
       61 agctcagcag gtgtaaactc aggctcaggc agagccatac cccacaatgc agcccgctgt
      121 cgaaaatcca gcgacggccg ccagacacca gtcatcggaa atttcccgat cggttcgctc
      181 aggccatccg ggtattcagg ttctgctgtc tgcaacggcg cgccattcga ctcactggct
      241 ggagcactct cgcgcatgcg cgcgttatgt gtggggttta attctttatc tgtatctgta
      301 tctgtcgtga cttgtcgtga catatgcgtg acgcgtcgtg actcatcgtg acaatcagtg
      361 ttctgctccc gcagtctttc ccgctcccgc tgcgctctct tgcgctctgc cggggatttt
      421 gcggtttgcg aaacattacc gttatcctcc ttcatcacct ggcgtttttc ccatccggaa
      481 ataagatcgc catccagaac tcgcccctgc attgcatgca aaattgaatc aatcacgtct
      541 tccgtcacat caagcgcact tgctaaatct tccgtcgtga catcaatgtg accacgtagt
      601 gactcgccgt gacatttcgt gacatttcgt gacgcactca ccagaaggtg gatatacacc
      661 gccatcactg ttgcgattgg ctgtcctgag accctggcaa tggttcgcca tttgggatca
      721 tttggcatgt catgccacaa tctgagccag gcattagcca tactcacctc atctgatacc
      781 gaactttacc ctcgaacatc cggaagaaat ccggcatgaa tattgttggt caatgcacga
      841 caacagcatt accaggctga cccccactgt tagtcagggt gccccaggcg atcgccgctg
      901 cgacaaaatc atccacatct ttcaccagcc gatccctccg ttcgacgatc tcacggtaat
      961 attcagagct gtgactgcgc atacgggcca ccagcagagg cggcattgcc ttttcgatcg
     1021 ccggtaacag agcctgcatt ttttcaacag catcaggggt gtctttatcc agccaacgga
     1081 aaattttctg ggtattacga gccagggctt ccggatggct gtcgtcgtac agttccggga
     1141 acgtcatccc cagttcgaaa taagtccggg ctatttcagc tgcaggaact ttctcaccat
     1201 caggatatgc ccaggcattc atcgccatgc ggatgtgctc atgtttgatt ttcatgaatc
     1261 atttgcctct tgatgcttcg ggtatgatcg ttttcgtcat ttggttgctt catcgacata
     1321 ttctgcgaat aacatgacga gcgtcgtaag tatgtccaat caacatcagg acgaagttct
     1381 tcacacagga caccaccttt tgttgctcgt tcaatcgcag gacatctctc agcaggcaac
     1441 tgacgtacac ctttgatcca ttgatttacg cttggaggag atacacctaa aagcctagcc
     1501 attgctgatt gcccaccgac aacagcacaa gctcgtttga atgaatagtt atcttttttc
     1561 atcgaatgaa ctccaaaaaa cacacaataa tattaggctt agcctaatgc aactgtcaat
     1621 aggctatgcc taacgcttcg agagtaggta ttgcctaatg cgatgcgcat aggagactat
     1681 taagcaatgc ttagtggtaa agacttaggc cgagcgatag agcaggccat taacaaaaaa
     1741 attgcatcag gagccgtcaa atcaaaggcg gaaatcgcac gtcatttcaa agtccaacca
     1801 ccatcaatcc atgactggat taagaaaggt tcgataagta aagacaaact tccagaacta
     1861 tggcgtttct tttctgatgt ggttggtcca gagcattggg ggcttaacga ataccccata
     1921 ccaaccccat ccacttcaga tacaaaaagt gaacttttag acataaacag cctttatcaa
     1981 gccgcctctg atgaaaaaag agcaattgtg gctttcctct tatctggaaa tgctacggag
     2041 cctagttggg ttgatcatga cgttcgtgcc tacattgccg caatggaaat gaaggtagct
     2101 aactatctga aaaatcaaga atcaaaacgg aaaagccaga acatcaccaa gacaggaact
     2161 taaacttata tggtccgacg ggaaattcct agttcccgtt agttaactcc tactacctct
     2221 cccacaaacc atcacctatt aggtcgcacc caaatcatta ggcgtagcct attgacaagc
     2281 aattaggcat ttcctatagt tttcccatac caacccatcc cgtcccacac aatacagggc
     2341 aatacctaga gttacccggc agtggtcagg gattaagtag ccagcccgag gcgtaagaac
     2401 atgacggcag ggttcaactt taactatgca gcaggttttt gttccgctac cccggcgtta
     2461 aggggatatg aggtcagcat ggatactctt gatcttggca acaacgaatc tctggtgtgc
     2521 ggcgtgtttc ccaaccagga cggcacgttc accgcgatga cgtataccaa aagcaaaacg
     2581 tttaaaaccg aatctggcgc gcgtcgctgg ttagcaagaa actctgacta atgaaatctg
     2641 gtaattaagg aatcctccac ggggggagag tggtgcacac gcgccggaca caagcaagca
     2701 tccggcatgc tctttaacaa tctggatatc cataacagta aaaatctaca gattgccgtt
     2761 cagttttctg gccaactctt caatagatgg aggtgatacg taatccggat ttttattcat
     2821 aagaaactga ttttcacagt acaggcacct gcttttatga aaaaattcat attctctaac
     2881 cgggaatggc tgaagtatcg atactatctt ttgtccaaaa cattttggac aaagatgcat
     2941 gattatgctg ccgccgttca cggttacctc cttcgagtac acaaaagttc ccgactcaag
     3001 ttggctaagg atataacctt ccgtctgagc ctcaaagttt tcaaattctg caattttagc
     3061 tttgagagaa gcatttattt cttgataagt gcccaccagc tcaacgagag acacgcattc
     3121 gcgctgaata gatgcaagct ttgagttcag ttcaccaata gccgcattta cttcagcttg
     3181 agtttttgcc tcgttcatta gttttgcaat ctgggcggtt tcacgaatag ccgtcattgc
     3241 tgccgttaat tcagcgatca cattcaatac tcttattgtt gttggggata tccagattaa
     3301 caagatcctt gttgttgggg aataacgggg accacctcgc ctgacgtggt aaaaagcagg
     3361 cacacaacac gaaagcacac ggcgagggac accattaaat ggtggcttaa gcaatcacgc
     3421 gaagcgcaat ctgccattat gaccgatgaa atcccgttag atgatgcact gttacaattg
     3481 cgggaattta tcgacgaaaa ctccggtgaa ttttttgttc aggtctgggg aaatggagcc
     3541 aacttcgaca acacgatttt gcgccgttca tacgaacggc aggggatccc ctgcccgtgg
     3601 cgttactaca acgatcgcga tgtacgcaca atcgttgagc tggggaaagc catagacttc
     3661 gatgccagaa cggctattcc attcgaaggt gagcgccata atgcacttga tgacgcccgt
     3721 taccaggcaa aatacgtttc agttatctgg caaaaactga tcccgagtca ggctgatttt
     3781 taatgttcaa ccgtcgccag ttgtcgttga tattctgcaa ctggcgcgtt ccggagtgat
     3841 agccatgagc gaacagtacc tgataacgct cgacgagtgg aaaccaaaac ggttcagtct
     3901 cccaataaca aacactaccc tggtgaaata cggaaaacta ggatacatcg ttccaagacc
     3961 acaaaaaatt cgtgggcgtt ggctgataga tcgccgagca gtatttgttg ggcctggtga
     4021 aacgggaatt gcgccggaaa ttcatactgg cgatgatgat gcactgaagg agattttaac
     4081 tcatgtcacc gaggccacga aaaaacagca ctgacgtagc cggtctttac gaaaagtttg
     4141 atcgcagaac tggcagagtt tactaccagt ataaaaatcc tgtgactgga aaatttcacg
     4201 gactcggaac agacaaaggt aaggcagaaa aaatcgcttc cacagccaat cagcgaatag
     4261 ctgcagcaga agctgaatat ttcatgcgca aaattgatga aagtccgtca gcaacaaaac
     4321 gtcggggtat cagattaaag gcatgggttg atcgatatct gaaaatacag gacacgcgac
     4381 tgaaaaatgg agatattgca gctacaactc acaaagaaaa aactcgaatg gctgcatacc
     4441 tggtttcccg tctgggaaac cacccattga aagaactgga agtaagagac tttgcattaa
     4501 tactggatga gtggctggat aaagacatgg tcagtacagc gagagtaaat cgtggattat
     4561 gggttgatat ttataaagaa gcacagcatg caggggaagt tcctcctgga tggaatcctc
     4621 cggaggctac ccgtaaaccg atccctaaag taaccagagc caggctcacc atggaagact
     4681 ggcaaaaaat ttacaatgca acgcctgaaa aacactttat ccgtaacgca atgcttcttg
     4741 cgattgttac tggtcagcgc cgtgatgaca tttgccacat gcgtttttca gatgtgtgga
     4801 acgaacactt gcatatcacc cagggaaaaa ccggaatgcg tctggcgtta ccgcttacac
     4861 tacgctgtga tgccattggg ataacgttaa aagaagttat tgatgggtgc cgagacagaa
     4921 tattaagtcc atatctaatc catagtcggc accagaaaca accgaagccg atgagtaaag
     4981 acaacctgag cgactacttt gccaaagcac gggatctggc tggggtaatt ccaccagcag
     5041 gaaaaactcc gccaacattt catgaacaac gctctctatc agaacggctg taccgtgcac
     5101 agggtatcga tacaaaaaca ttactaggac ataaagtcca ggcaaccacc gatcgctata
     5161 acgatactcg aggtcaggaa tgggttaagt tggttatttg acaaacacca tatggaatat
     5221 ccgtcaccat ctcaggaaaa aagtgttgta aaatgcgtgg cctagttttg gggaaacgtt
     5281 ttggagagat tttggagaaa ggaaaaaatt gaataaattc aaaatcctga aagtgtcccg
     5341 ccttatgaat aaaaaggtgg aactcgaatg atcggtcgac tgcttcgcgg cggttttatg
     5401 accgctatct acgcgtacct gtatatccca atcattattt tgattgtgaa ctcctttaac
     5461 agctcgcgct ttggcatcaa ctggcagggt tttaccacca aatggtatag cctgctgatg
     5521 aacaacgaca gcctgttaca ggcagcgcag cattcactaa caatagcggt gttttcggcg
     5581 acgtttgcta cgcttatcgg ttcactgacg gcagttgcgc tgtaccgtta tcgctttcgt
     5641 ggtaagccgt tcgttagcgg aatgctgttt gtggtgatga tgtcaccaga tatcgtgatg
     5701 gcgatttcgc tgctggtgct gtttatgctg ctgggtattc agcttggctt ctggtcgctg
     5761 ctgttctcgc atatcacctt ctgcctgcca tttgtggtgg tgacggtgta ttcgcgcctg
     5821 aaaggttttg acgtgcggat gctggaagcg gcgaaagatc tcggtgccag cgaatttacc
     5881 attctgcgaa aaatcattct gccactggca atgccagcgg tggcggcagg ctgggtgtta
     5941 agctttaccc tgtcgatgga cgatgtggtg gtttcttcgt tcgtcaccgg gccgagttat
     6001 gaaattctgc cgttaaaaat ttattcgatg gtcaaagtcg gcgtatcgcc ggaagttaac
     6061 gcgctggcaa ccatattact ggtgctgtcg ctggtgatgg ttattgccag ccagcttatt
     6121 gctcgtgata aaacgaaagg taacacaggg gacgttaaat gaaaaaatgg tcacgccacc
     6181 tgctcgcggc gggtgctctg gcactgggca tgagcgccgc tcacgccgat gacaacaaca
     6241 cgctgtattt ctacaactgg accgagtacg tgccgccagg actgcttgaa cagttcacca
     6301 aagaaaccgg tattaaggtt atctattcga cttacgagtc gaacgaaacc atgtatgcca
     6361 agctgaaaac gtacaaagac ggtgcctatg acctggtggt tccttcaacc tattacgtcg
     6421 ataaaatgcg taaagaaggg atgatccaga agatcgacaa gtcgaagtta acgaacttca
     6481 gcaatctcga tccagacatg ctcaataagc cgtttgaccc gaataacgat tactccattc
     6541 cgtatatctg gggtgcgacg gcgattggcg ttaacggtga tgcggtggat ccgaaatctg
     6601 tcaccagctg ggccgatttg tggaagcctg agtacaaagg cagcctgctg ctgaccgacg
     6661 atgcccgtga agtgttccag atggcgctac gtaagctggg ctactccggt aacaccaccg
     6721 atccgaaaga gattgaagct gcatataacg agctgaaaaa actgatgcca aacgtcgcag
     6781 cgtttaactc cgataacccg gctaacccgt acatggaagg cgaagttaac ctcggcatga
     6841 tctggaacgg ttctgctttc gttgcacgcc aggcgggtac gcctattgac gtggtgtggc
     6901 cgaaagaagg cggcatattc tggatggaca gcctggcgat cccggcaaat gccaaaaaca
     6961 aagaaggcgc gctgaaattg atcaacttcc tgctgcgccc ggatgtggca aaacaggttg
     7021 ctgaaactat cggttatcca acgccaaacc ttgcggcgcg taagctgtta agtccagaag
     7081 tggcgaacga taagacgctc tccccggatg ctgaaaccat taaaaatggc gaatggcaga
     7141 atgacgttgg cgcagccagc agcatttatg aagagtatta tcagaagctg aaagcaggac
     7201 gttaattcag caagcgggcg gtggtattac cgcccgtttt ttagttagca aggatgtggc
     7261 taatgagtca ggacagtaaa gttttatccc gctctttcgt cggtattgtg gtccgcttgt
     7321 aggcctgata agcgtagcgc atcaggcaat gcttcctgct ttataatccc ttcagcaact
     7381 tttcaacaaa ttctggcacc acctggcttg ccgggccgta atatttctcg gcaaattcat
     7441 taccgacctg acttggttca agattcagct ccacggtgtg cgcgccatgc agtttcgctt
     7501 cgtgaacaaa cccagccgcc ggataaacat gcccggaagt accaatggca atgaaaatat
     7561 cggccatcga caacgccata taaatttcat ccatgccgag tggcatttcg ccaaaccata
     7621 ctacgtgtgg gcgcaagggg gccgggaact ggcagcaatg gcatttatct tctggggtaa
     7681 cgtctccggt ccagtcgaga acctgaccac tttgtgaaca acgaactttc agcagttccc
     7741 catgcatatg aatcacattg gtattacctg cgcgttcatg caggttgtct atattctgcg
     7801 tcaccagcaa aaagcgatcg ccgagagcat cttgcagttt agccagcgca agatgcgcgg
     7861 cgttaggctg aatttctggc tgctgcagct gtcgacgacg ggcgttataa aacgcttgca
     7921 ccagttcagg atcgcgatcg aaaccttccg gcgttgccac atcttcaacc cgatgttctt
     7981 cccacaggcc atctgcggcg cgaaaggtac gaatacctga ttccgcagaa attcctgccc
     8041 ctgtcagtac gagtactctt ggtttttcca ttgcttccgg caccacttta tctctgaaaa
     8101 aaatacgctg acgcaaacgc tcgcgcaggc ggcgtttatt tttacgaaaa cgacttaacc
     8161 gatgaccccg acgcgacagc atagcaacct ctgttgttta atcggttaga tgtaggaagg
     8221 ccgcaccacg cattcctccc gcatcaccgt ggcgcgcgcg ttcaatgcgc ggaacacgag
     8281 ctacaggtaa gagatgacga ggcagcctgt ccgccagttg cgttgtgatt gccgggaaat
     8341 tcgataagcc accaccaatg acgaccaggt cagggtcaac aatggtcagg atatttccca
     8401 gacaaaccgc taataaatcc agataacgct caacgtgcgc ccttgcctgc tcatcgcctt
     8461 gatcataaag cgcaataatt tcgggagccg gcaacggttg atgataatag tgttgataca
     8521 gccacgcaaa accgcgacca gacagataat tttcaatgca gccatgctga ccacagccgc
     8581 agcggcgtaa cgggaaatcc agccccatca tggttaacgc atcaaccggc agacgcatat
     8641 ggccaaactc gccggtaatg tagcttttcc cggtaatcgg tttgccgttg aaaatcagcc
     8701 cgccgccaac gccggtgccg agaatcaacc ccatcaccaa cggatattgc gtaaattcgt
     8761 catcccaggc ttctgaaagg gcaaaacagt tggcatcgtt atcaaggcgt acatcgcgat
     8821 caagacgtgc gctcaggtcg gcacgcagcg gtttaccgct ggcagcaggg acattggcgg
     8881 catacagcgt accatcttct gtttccggca ttcccggaat accgatgccg acagagcctt
     8941 tacagccaaa acgttgatca gcttcggcta ccagctcgca cactgcatct aaaaatgcgt
     9001 catagctgtc acgcggtgtc ggcacccgct tttcccactg caactgccga ccgctatcaa
     9061 acacgccaag cgcaattttt gttccaccaa tatcaaaccc gtaatacatt gccgctcctt
     9121 gttttaatgt actgccttta ctggccgcta aggactcgcg cagggtcaat attactggcg
     9181 cgccgcgccg gataccaact tgccaaaaga ctcagcaaca atgctgtgac cagtacgtag
     9241 aagacgtcca gccagtgcaa ttccgatggc aggaagtcaa taaaatagat atcgctggag
     9301 aggaactgat gaccgatcag cttttcaatc cgctcaataa tcggggtaag ttgcagtgaa
     9361 acaactacgc cgataatcac accacacagg ctaccgaata gccctgccag caatccatac
     9421 cagacaaaga tggcgcgaat taaaccatct ttcgccccca gcgttcttaa tactgcgata
     9481 tcgccactct tgtctttcac cgccatcact aaggtggaga cgatgttgaa acaggccacg
     9541 ccaatcacca gtaccatcgc cagatacata atggcgcgga tcatctggat atcgcgatac
     9601 atatagccgt aagtaccaat ccagctttta atataaacat agctgttggt cacttcaccc
     9661 gcatcgcgta ccagcttatt ggcgttgaaa acatccgtca ttttaagggc aatacctgac
     9721 acgctggaac ccatatcaag atattgttgg gcatccgcca gcgggatcat ggcaaaactg
     9781 tgatcgagtt gaccactcaa ctgcaaaata ccggcaacgt gcaaacgcac acgttttggc
     9841 tgcatcagtt tatgctcagg attcgagttg gggatcataa tcgacaccca atcgccctgc
     9901 ttcactttca gcgcatccgc cacgcctttg ccgatgataa tttgctgttc gcccgcttta
     9961 aaattgcgcc aggcatcccc ctgaacaaac gagggtaatg cgctcagacg ctgttcctgt
    10021 tgcgggttaa cgcccttcac ctggattgcg cgcagattcg ctccactttc caccagcccg
    10081 gtgaaattga tatacggcgc agcggcggca atacctggca ctttttgcac gttatccagt
    10141 gcttcctgcc agttagtcca cggttgatcc accgcctcta tttcgccatg cggcaccacc
    10201 gccagaatgc ggttattcag ttcgcgttca aagccgttca tcgcgcttaa gccgacgatc
    10261 aataccgcca cgccaagggc aatgccaatg gtagaaatga cggagatcag cgacaccatg
    10321 ccgccgcgtc gccgtccgcg gctaaaacgc aggccaatca ataacgataa aggcatcgcc
    10381 attactccgc ccccatcagg ctcagttccg ccgtcagacg accatcacgc atctccagtt
    10441 ggcggctcat acgtttcgcc agttgcaggt cgtgagtaac caccaggaag gcggtgccct
    10501 gcaagcgatt caattcccca agcaactgga agatgctgtc tgcgttacgc gcatcgaggt
    10561 taccggtagg ttcatccgcc agtaccagcc acgggttatt gaccagcgca cgggcaatcg
    10621 ccacacgctg gcgttcgccg ccagaaagtt cagatgggcg gtgattcgca cgatgctcca
    10681 gccccaccgc ttttaacatc tcaagtgcac ggctgttgat ttcagcgggc tttttcttgc
    10741 caatcagcag cggcatagcc acgttttcca gggcagtaaa atccggcagc aggtggtgaa
    10801 actgataaat aaagcccagc ttctggttgc gcagttctgc tttcgccgcc gaagacagtt
    10861 tgctcatcgg ttgaccgtta aagatcacat cgccggaggt tggtgtatcc agcccgccca
    10921 gcaggtgcag caaggtactt ttaccggaac cagagctacc gacgatcgcc atcatttcac
    10981 cttcgcccac gctgaaactg acattgtgca gtacatcggt ttgcacactg ccttcctgat
    11041 agcgtttgca caggttgtcg cattgcaaca ggatcttatt cataacgtaa agcctcagcg
    11101 ggttgtgtgg cggcagcgcg ccatgaaggg taaagcgtag acagcagcgc gatagccatc
    11161 gccaccagcg caataacaat gacctgtaaa ggttcgatag ccaccggcag cgccgcgcca
    11221 tcaagcagga cgccgattat cggcatcaga ttatttaact ggctggcaag cagtgcgcca
    11281 agcgccgctc cgaggatcgc accgataatc ccggcgctgg ccccttgcac cataaagacc
    11341 atcatgattt gtcgcggagt taagccttgc gtttgcagga tcgctacttc gccctgcttc
    11401 tccatcacca tcagccccag cgaggtaata atgttaaacg ccgcaacggc gacaatcagg
    11461 ctcagcagta agcccatcat atttttttcc atgcgtacgg cctggaacag ctcgccttta
    11521 cgatcacgcc agtcctgcca tttgctgcct tcaggcagtt tttgctgact taaagagtca
    11581 actttcagcg gctcatccag ccacaaacgc cagccggtaa tattgcctgc cggataacgc
    11641 atcagacgcg aggcatcctc aatattcacc agcatttcat agccatcgac ttcactgtta
    11701 gcggcaaaag taccaatcac attgaacagg cgctgacttg gaatacgccc catcggcgtg
    11761 aactggctgg cagatggcac catcacgcgg atttgatcac cgcgattaac gcctagctgt
    11821 gaggcaagtt gttcgccgag gatgacatta tatttccccg gctcgaggtc agtttgtttc
    11881 acattgacca gatacggcgt tagtggatct ttttgcgccg gatcgatacc cagcatcacc
    11941 ccgaccgcca cgctgcgcgc gctttgcagt accacatcac cggtagtaat aggtgcgacg
    12001 cgattaacgc cgtccagttt gactgccgtt tccgggagtt gctgcgggtt aagagagcca
    12061 tgctcagaag agagaattgc ctgtggcatc aggccaagga tgttgttttg cagctcgcgc
    12121 tcaaagccgt tcatcactga caatactgtg accagcgcca tcaccccgag ggtaatgccg
    12181 atggtagaaa gccaggagac gaaacgaccg aagcgatccg ctgcacgccc acgcatgtaa
    12241 cgcaggccaa taaatagagc gacaggttgg tacatgaaat ccgtctggtt gctgtagcaa
    12301 agacacgagt atataagcga aagcaagatg ggtaaacgtc tgaactgtaa gtgatcgtca
    12361 gggctaatct gaaaagcccc cgtaaatcaa tcggctaaat tgccatgttt tatcatcaat
    12421 cttgccgcct gatcttaatc tgaaaaatca tccaatacag acagataccc attacacctc
    12481 gggtcataac ccgcaggatc aaccgcatgt aaattaaaaa agagcgtgtt gtcctgatga
    12541 aacaaaaaga gctatggatt aaccagatca aagggttatg tatttgtctg gtggtgattt
    12601 atcactcggt cattaccttt tatccgcata tgaccacttt tcagcatccg ttatcggaag
    12661 tcctgagcaa atgctggatc tatttcaatc tttaccttgc accctttcgt atgccggttt
    12721 ttttctttat atccggctat ttgattcgcc gctatatcga cagcgtaccg tggggaaatt
    12781 gtctcgataa acggatctgg aacatcttct gggtgctggc gctttggggc gtggtgcagt
    12841 ggctggcgtt aagtgcgctg aaccagtggc tggcacctga gcgcgattta agcaatgcct
    12901 ccaatgccgc gtatgccgat tccaccggtg agttcctgca cgggatgatc accgccagca
    12961 ccagcttgtg gtatctgtat gcgttaattg tctatttcgt ggtatgtaaa atttttagcc
    13021 gtctggcgct gccactattc gccttgtttg tactgctgag tgtggcggtt aatttcgttc
    13081 ccacgccgtg gtggggaatg aacagtgtga tccgcaattt gccttattac agccttggtg
    13141 catggtttgg cgcaacaata atgacctgtg ttaaagaggt gccgttgcgc cgccatctgc
    13201 tgatggcttc tttgctggct gctctggcgg tcggtgcctg gttgtttact atctcgctgc
    13261 tgttgtcgct ggtgtcgatt gtggtgatca tgaagttgtt ttatcagtat gaacaacgtt
    13321 ttggcatgcg ttccaccagt ctgctgaatg tgattggctc caacactatt gctatctaca
    13381 ctacccatag aattctggta gagatattca gcttaactct gcttgcgcaa atgaacgcag
    13441 cacgctggtc gccgcaagtc gaactgacac tcctgctggt ttaccccttt gttagtttgt
    13501 tcatctgtac tgttgcgggc ttgctggtaa gaaaactttc acagcgagca ttcagcgatc
    13561 tgttgttctc cccgccttca ctgcccgttg ccgtcagtta ctcccgctaa gccatcgccc
    13621 cagccggggc gattcaattt gctaaaccat gtcgattacg gataatgaac agcattgctt
    13681 atcagagata tgcccccata tgttgaggca tatcctaacg agaatctgac aaccgttatg
    13741 cctgaacaat atcgttatac gctgcccgtc aaagcgggtg agcagcgtct gctgggcgag
    13801 ttaaccggcg ctgcctgcgc gacgctggta gcggaaattg ccgaacgtca cgccggaccg
    13861 gtggtgctta ttgcgccaga tatgcaaaat gccctgcgtt tgcatgatga aattagccag
    13921 tttaccgacc agatggtgat gaatctggcg gactgggaaa ccctccctta cgacagtttt
    13981 tcgcctcatc aggacattat ctcctcgcgc ctttccaccc tttaccagct accgacaatg
    14041 cagcgtggcg tactgattgt tccggtgaat acacttatgc agcgcgtttg cccgcacagt
    14101 tttctccacg gtcatgcgct ggtgatggaa aaaggtcagc gcctgtcacg agatgcatta
    14161 cgaacccaac tggatagcgc cggttatcgc catgttgacc aggtgatgga gcacggcgaa
    14221 tacgccacgc gcggcgcgtt gctggatctc ttcccgatgg ggagtgagtt gccttatcgt
    14281 cttgatttct ttgatgatga aatcgacagt ctgcgggtgt ttgacgtcga cagtcagcgc
    14341 acgctggagg aagtagaagc gatcaacctg ctgcctgcgc acgaatttcc gaccgataaa
    14401 gcggcgattg aactgttccg cagccagtgg cgcgatacct tcgaagtgaa gcgcgatccg
    14461 gaacatattt accagcaagt gagtaaaggc acattacctg ccgggatcga gtactggcag
    14521 ccattgttct tcagcgaacc actgccgccg ctgttcagtt atttccctgc caataccttg
    14581 ctggtgaata ctggcgatct ggaaaacagt gccgaacgtt tccaggctga cacgctggcg
    14641 cgttttgaga atcgcggcgt cgatccgatg cgcccactgt tgccaccaca atcgctctgg
    14701 ctgcgggtgg acgagctctt ctcagagctg aaaaactggc cccgagtgca gctaaaaact
    14761 gaacatttac cgacaaaagc cgcgaatgcc aatttaggtt tccagaaact gccagacctg
    14821 gccgttcagg cacaacaaaa agcgccgctg gatgcgctgc gtaagttcct cgagtctttc
    14881 gacggtccgg tggtgttctc ggtagaaagt gaaggccgcc gtgaagcgct gggtgaactg
    14941 ctcgcgcgaa ttaaaattgc tccgcaacgc attatgcgtc ttgatgaagc cagcgaccgt
    15001 gggcgttatc tgatgattgg cgctgcagag catggttttg tcgatacggt acgtaatctg
    15061 gcgctgattt gcgaaagcga tctgctcggt gaacgcgttg cccgtcgtcg tcaggattct
    15121 cgccgcacca tcaaccccga tacactgatc cgtaacctcg cggaactgca tattggtcag
    15181 ccggtggtcc atctggagca cggcgtcggg cgctatgccg gaatgaccac gctggaagca
    15241 ggcggcatta ccggcgagta tctgatgctc acctatgcca acgacgccaa attgtatgtt
    15301 ccggtgtcgt cgctgcatct gattagccgt tacgcgggtg gtgcggaaga aaatgccccg
    15361 ctgcataaac ttggcggcga tgcgtggtca cgcgcgcggc agaaagcggc ggaaaaagtg
    15421 cgtgatgtgg cggcggaatt gctggatatc tacgcgcaac gcgccgccaa agagggcttc
    15481 gcgtttaaac acgatcgtga gcagtatcag ttgttctgcg acagcttccc gtttgaaacc
    15541 acgccggatc aggcgcaggc cattaatgcg gtacttagcg acatgtgtca gccgctggca
    15601 atggatcgtc tggtgtgcgg cgatgttggc tttggtaaaa cagaagtggc gatgcgcgcc
    15661 gctttcctgg cagtagataa ccacaagcag gtggcggtgc tggtgcctac cacccttctc
    15721 gcgcagcagc attacgacaa cttccgcgac cgtttcgcca actggccggt acgtatcgaa
    15781 atgctctccc gtttccgcag cgccaaagag cagacgcaaa tccttgcgga agtggcggaa
    15841 gggaaaatcg atattctgat cggtacgcac aaactgctgc aaagtgacgt caagtttaaa
    15901 gatttaggcc tgctgattgt cgatgaagaa caccgcttcg gggtgcgtca taaagagcgc
    15961 attaaagcga tgcgcgcgaa cgtggatatt ctgacgctta ctgcaacgcc gatcccacgt
    16021 acgctgaata tggcaatgag cggaatgcgt gacctatcga ttatcgccac gccgcccgcc
    16081 cgtcgtctgg cagttaaaac ctttgtccgt gagtatgaca gcctggtggt ccgcgaggcg
    16141 atcctgcgtg aaattttgcg cggaggacag gtttattatc tctacaatga tgtggaaaac
    16201 attcagaaag ccgccgaacg gctggcagaa ctggtgccag aagcgcggat cgccatcggt
    16261 cacgggcaga tgcgcgagcg cgaactggaa cgggtgatga atgatttcca tcatcaacgt
    16321 ttcaacgtgc tggtttgtac aaccattatc gaaaccggga tcgacatccc gacagccaac
    16381 accattatca ttgaacgcgc ggatcacttc ggtctggcgc aactgcacca gttacgcggt
    16441 cgcgtcggac gttcgcatca tcaggcatat gcatggctgc tgacgccgca tccaaaagcg
    16501 atgactaccg atgcacaaaa acgtcttgaa gcgattgcct cgctggaaga tctcggggca
    16561 ggttttgcgc tggcaacgca cgatctggag attcgcggcg cgggtgaact gcttggcgaa
    16621 gaacaaagcg gctcaatgga aaccatcggt ttctcgctgt atatggagtt gctggaaaac
    16681 gccgtcgatg cactgaaagc cggacgcgag ccgtcgctgg aagatctcac cagccagcaa
    16741 acagaagtcg agctgcggat gccgtcgcta ttgccagatg atttcattcc tgacgtgaat
    16801 acgcgtctgt cgttctataa acgtattgcc agcgccaaaa cggaaaacga actggaagag
    16861 atcaaagtcg agcttatcga tcgcttcggc ctgctgccgg atccggcgcg taccctgctg
    16921 gatattgccc gtctacgcca gcaagcgcag aaactgggga tcaggaagct ggaaggtaat
    16981 gagaaaggcg gcgtgatcga atttgccgag aagaatcacg ttaatccggc ctggttgatt
    17041 ggtttgctgc aaaaacagcc gcagcattac cgccttgatg gtccgacgcg cctgaaattt
    17101 attcaggatt tgagtgagcg gaaaacgcgt atcgaatggg tacgccagtt tatgcgtgaa
    17161 ctggaagaaa acgcgatcgc ttaatggtaa atcgggggcg tttctgcgcc cccatctgca
    17221 acatttacaa attctttgca cttccctgca ctatccgaca ctgtcaccat ccataattca
    17281 ggcttatctg tttattacaa taaccttata tttattatgg atttttggtg atgatcaaaa
    17341 cgcgtttttc tcgctggcta acgtttttta cgttcgccgc tgccgtggcg ctggcgctac
    17401 cggcaaaagc caacacctgg ccgctgccgc cagcgggcag tcgtctggtt ggcgaaaaca
    17461 aatttcatgt ggtggaaaat gacggtggtt ctctggaagc catcgccaaa aaatataacg
    17521 tcggctttct cgctctatta caggctaacc ccggcgttga tccttacgta ccgcgcgcgg
    17581 gcagcgtgtt aacgatcccg ttgcaaaccc tacttccaga cgcgccgcgc gaaggcattg
    17641 tgatcaacat tgcggagctt cgtctctatt actacccgcc gggtaaaaat tcggtaaccg
    17701 tgtatccaat aggtattggt cagttaggtg gtgacacgct gacaccgaca atggtgacca
    17761 ccgtttctga caagcgtgca aacccaacct ggacgccaac ggcaaacatt cgcgcgcggt
    17821 ataaggcaca aggaattgag ttgcctgcgg tagtgccggc tggaccggat aatccaatgg
    17881 gccatcatgc gattcgtctg gcggcctatg gcggcgttta tttgcttcac ggtacgaacg
    17941 ccgatttcgg cattggcatg cgggtaagtt ctggctgtat tcgtctgcgg gatgacgata
    18001 tcaaaacact ctttagccag gtcaccccag gcaccaaagt gaatatcatc aacactccga
    18061 taaaagtctc tgccgaacca aacggtgcgc gtctggttga agtacatcag ccgctgtcag
    18121 agaagattga tgacgatccg cagctgctgc caattacgct gaatagcgca atgcaatcat
    18181 ttaaagatgc agcacaaact gacgctgaag tgatgcaaca tgtgatggat gtccgttccg
    18241 ggatgccggt ggatgtccgc cgtcatcaag tgagcccaca aacgctgtaa atcctggata
    18301 aaaaaaatcc ccgcgacacg gcatgtcgcg gggaaaaaat cagcagtagc aggcattaat
    18361 gagggttaat gcttatttat aaattactgc tgttccatgg agggtattcg gaccggttac
    18421 agaagtaata cggaaagatt ttgcgcccat ctcatccgct ttttgcgcca gctgctcttc
    18481 cagcgatccc agatttgtcc ccgcgttagc actgattgta ccgacttttt gttggccttc
    18541 tggcgttgac tgaacttcga cagccgcaaa gctggcaaat gacatggagc ttaaaatcgc
    18601 cgcagcgatg agggttttta cgtttttcat aatagtggcc ttatgcagat gaatgacgtc
    18661 gcaagcaatt acttaacgat cgataactaa atgatagatg tgatctggat cacatacaag
    18721 atatttttta taacaatcat taattaattt aaaaatcttt aaatttcagt taaatagaaa
    18781 gcaattattt ttatgggatc tggactggtg aacggaggtg attattttta taataacctt
    18841 tcattcacag tcaccaggta caacggcatc atggcaactg actcaacaca atgtgtaaaa
    18901 aaaagccgtg gccgcccaaa agtgttcgac agggatgccg cgcttgataa ggccatgaaa
    18961 ttgttctggc aacacggtta tgaagcgact tctcttgcgg acctcgtcga agcgaccgga
    19021 gccaaagcgc ccacgctata cgcggaattt accaacaaag aggggttatt tcgtgccgtt
    19081 ctcgaccgct atatcgatcg ttttgccgct aagcatgaag cacagctgtt ttgtgaagag
    19141 aaaagcgtgg agtctgcgct ggctgactat tttgctgcca tcgccaactg ctttaccggc
    19201 aaagacaccc cggcaggctg cttcatgatc aacaactgca ccaccctctc cccagattca
    19261 ggagatatcg ccaatacgtt gaaatcacgc catgcgatgc aagagcgcac tttgcagcag
    19321 tttttatgtc aacgacaagc gcgcggggaa atcccgaccc actgtgacgt gacacatctg
    19381 gcagaattcc ttaattgtat tattcagggg atgtcgatca gcgcacgcga aggtgcatcg
    19441 ttggaaaaac tgatgcagat tgccggaacg actttgcgtt tatggcccga actggtgaaa
    19501 taagtcgcaa aaaaagcccc tcagcctgag cgaatgaggg gccaaattgc agagtacagc
    19561 cttgttatac tttgttattc aaaatcagtt gcccgttact atctagcggg atctgcgtac
    19621 ccggatcgcg gtccatgcgg attttgccct gctgatcgcc aatcttatag gtcacatcat
    19681 aaccgagcat tttttctgac ttgtcataca ccgttttaca acgctgttgc gtagtcgtgt
    19741 aagtatcgct ttcctggaga gagccctgga tctggttacc ggcatatcca ccacccagcg
    19801 cccccacaac agtggcgaca tctttaccac gaccaccacc aaactgatgc cctatcacgc
    19861 cgccagcaac agcgccgagc accgacccgg taatgcgatg ttcatcctgc accggtcgac
    19921 gatgggtcac tgtgacgttg cgacactcct gacgcggtgt tttaaccgtt tccttgattg
    19981 gggttgcaga aacaacctga gcgtattgcg ggccacgttc aaacacgttc agactggcca
    20041 ctgccgctac gccaagcgca gctgcgacac caatcccgat acccgccaac attgatttat
    20101 tcacgggaat acctccttct ttgcagttat gccaatcatg caagcgaagg agaaaagcag
    20161 ctatcagaca gaagatgaaa agcgcgggaa tggctggaaa tacttaagga ttcggaggag
    20221 tctgaaggaa tgcctgatgc gacgcttaac gcgtcttatc aggcctaccg ctcgtaccat
    20281 ttgtccaggg cggatgaggc gtttatgcca catccgccag tgtacgtcga ttaatgcaac
    20341 ttcaaacgcg gacggataac gcggttaata ctccccacca gcatcattaa tccggtttta
    20401 aagtaaccat gcagcgcaat ctgatgcatt cggtatagcg agatatatac aaagcgcgca
    20461 attcgtcctt caatcatcat tgagccgcgc gtcaggttac ccatcaggct accaacggtg
    20521 gagaagttcg acagcgatac cagcgaacca tgatctttat actgataatt tttcagcagc
    20581 ttaccgttca tctgcgccag aatgttgttc attgcgcaag tcgccatctg gtgtgcagcc
    20641 tgagcacgcg gcggaacaaa gcccccttcc ggacgcgggc atgacgcgca gtcgccaata
    20701 gcgtaaatgt ctggatcgcg ggtggtttgc agcgtcagtt ccaccaccag ctggttgata
    20761 cggttagttt caagaccacc gatatctttc aggaagtctg gcgctttgat cccggctgcc
    20821 cataccatca gatcagcctc aatatattcg ccatctttag tgtgcaggcc gccttcatca
    20881 gcactggtga ccatggtttg cgtcagaacg cgaacgccaa gtttcgttag ctcgttgtgg
    20941 gccgcagcag agatacgtgg cggtaatgca ggcaaaatac gttctcccgc ttctaccagc
    21001 gttacgttca gggcttcgtt ggtcaggcct ttgtaaccgt agctgtgcag ttgcttgacc
    21061 gcgttgtgca attcagcgga gagttctaca cccgtcgcgc cgccgccgac aatcgcaatg
    21121 ttcactttgc catttgcgcc caggttggcg gagtatttca ggaagagatt cagcatctcc
    21181 tggtgaaagc gacgcgcctg gtgcgggtta tcgaggaaaa tgcagttctc tttgacacct
    21241 ggtgtattga aatcgttaga ggtgctaccc agcgccatta ccagggtgtc ataggcgatt
    21301 ttacgttccg gaaccagcag ttcacctttc tcatcgcgca gttctgcaat agtgattgtt
    21361 ttcgcgtcac gatcaatatc aatgacggaa cccagctgga actggaaacc atgattgcgc
    21421 gcatgggcca gatagctcaa cgcatcgacg ccttcatcaa gcgagccagt cgctacttcg
    21481 tgcagcagcg gtttccacag gtggctgtgg ttacgatcga ccagcgtaat tttggctttt
    21541 ttcttgcgtc ccagcttatg ccccagctgt gttgccattt ccagcccacc agcaccgccg
    21601 ccgacaatca caattttttt caatggcgta gtcaacgtga cccccttaaa tttattaacc
    21661 aattgttaat taatggttat taacatagct cttaattaac aacaggttac agcaatgaaa
    21721 atgttcttcg ctattgagaa taacatgaat ggtgcattgg tcataccaaa attgatgtta
    21781 atcaagtttt gttgctgaaa agataggcga ctttaccggg tgagatcaaa aaacagagtt
    21841 tattgtttat tatttgagcg aatttgtgag agtgatatag cgtgaggggt gtatgtaggg
    21901 cgttcggtag gtctgataag aggcgcatca ggcattgatg ctcgactgtc ggatgcggcg
    21961 tgaacgcctt atccgactta cagaattcgg agcgcatcag gtaccggcaa ggttacctga
    22021 tgcgacgagg gcatttaccc gagggttttg aacgctttaa tgcgctgcaa atgcggggag
    22081 atattcttga atttgtgcgt ctgctcttcg tcccagacaa tctcgtaata atgatgcaac
    22141 tcttcagatg tccgctggct gttaagcgct tcatcattac gcgacaaaat caccaggcaa
    22201 cgatcgcgat tcttctcgcg gaagttggtc acacacttag tggcaatatc ggcatactct
    22261 tccgggcgat caatcttgcc ttccatgttc tcataaggga acaaattagg gttgaagatc
    22321 acctggcgga tatcgcaaag aaaaccaatc cgttccgccc agtatccgcc cagaccaacg
    22381 ccgcaaatca ggggacgctc gtcgacgttc agttgcaaca ttttgtccac ttctttgagc
    22441 agatgctgca tatcatgttt cggatgccgc gtactgtagc ttatcaagcg tacatccggg
    22501 tcaataaact gcaattgtaa gactttctcg tggttacccg gactgttaga gtcaaaaccg
    22561 tgtaaataga taatcatcgc ttcctcacat aagccagggt tagtgacctg ctttctcttc
    22621 ctgccagcgt tcatgtaact gattcagacg ggcgctgatc gctttccagc gagccgagtc
    22681 catcagttcc tgtcgcgaaa atgaaccttt atgatacaaa cgtgtaacac gttctgcctt
    22741 gatcggtgac agattatcta acacgctgac ggccccttta cgattattgc agaccaggat
    22801 catatcgcaa cccgcatcca gtgatacctg cccgcgttcg gcataactgc ccataatcgc
    22861 ggcaccttcc atcgataaat cgtcagagaa aatcacgccg tcaaaaccca gttcctgacg
    22921 caaaacggtt ttcagccagt aggaagaacc gctcgccgga cgcggatcaa catcactgta
    22981 gatcacatgc gcaggcataa tggcgtcgag tttattttcg cggattaacg tgctgaaaac
    23041 cgacatatct ttggcacgaa tttccgcttg cgggcgtggg tcgcacggcg tctctttgtg
    23101 tgaatctgca gttactgcac cgtgtcctgg gaagtgtttc ccggtcgttt tcattccggc
    23161 ttcatgcatc ccatcaataa accgactggc gattgccagg gctttttctg ggtcggcatg
    23221 ataagaacgc tcgccaatcg ccgcgctgat atgtcctaca tccagcactg gcgcaaagct
    23281 gatatcaata tccatagcga tcatttcgct ggccatcagc caacccgcct cttgcgccag
    23341 tttgccgccc tcttccattc ccaacagcgc agcaaaggat tgtgctgccg gtaagcgggt
    23401 aaaaccttcg cgaaaacgct ggacgcgtcc accttcctga tctaccgcca ccaccagatg
    23461 attgcgcgat gctgcgcgga tctggcgcac cagttcacgc aactgggcag gatcatgata
    23521 gtttcgcgta aagagaatca gccctcccac cagcggatgc gccagtattt cacgctcttc
    23581 cgcgtccagt tcgtaacctt cgacatccaa cattactgga cccacactgc tctccttatt
    23641 gttttattaa tagctgccgc caggtgtcat cggccagcct gataaattgt tgatcgccgg
    23701 tttgtcgcca gcggtactca aaccaccctg ctttgagcat cagcagccag ggaaaccatc
    23761 gcctgacctg acgccataat tgcgccggat aaatcttcgc gcgagtggca tagtcattga
    23821 ccaattgccg gtgctgttca gtattttcca cccacaccgc cgccagttcc agcgcgatat
    23881 caccatctcc tgcatactcc cagtcgatga gttttaaccc tgacgcgcta tgcactaaat
    23941 ttccggcgtg gacatccata tgcaatggac ttaagcgtaa aggccgtggt tcccgcgctt
    24001 tgcgcagacg ttttaacatt cgcagccaac ccactgtccg ccgcgccgga tcgctttgct
    24061 gccagtacag ttccagtaac ggcaacagcg ttattcgcca gccaaaacgt ggttgttgat
    24121 gtagataata cagcaagcct gccagttcgt tggtatccgg caaatacgtt tttacctcgc
    24181 cgggcagata gtcgactacc atccagtcac ggagatataa atgcggcttc ggtgcaatgc
    24241 atgcgggtag ttgtgataaa gcccgatact ggtgcaagaa cgcggactgc ggcgcatcag
    24301 gatcgtgcgg ctgacgcaca acaaaacgct ggccctgatg ttcaatgaga aaactaccgc
    24361 cactaagccc actattaaac gtcgtgacgg gatgaaactg cgggaaaaag cgcgacagca
    24421 attcgtcgcg cgtgatggga ttattgctgc gaaacggcac ctttacctga ccagataatt
    24481 tcgcccgtct gcaccagcat cagctgcatt tgtagggtcg gagcgttaac gttgccagag
    24541 gcgctggagt acagcacgta atgagcgccg acattgcggg caatgcctat ggctttacta
    24601 cgggtgccta aactatcctg cggcgacaaa cctaactgtt gcttagccat cgacagctgc
    24661 tgggcggaaa ccagggtaaa tttcccgtta ttagccagcg catttcgcag cgtttcggtc
    24721 gcttctgcgg cattcagcga accgttagta cggttgttaa cgctatcaac cagcaggacg
    24781 ctacccgcag tcaccccgtc agccccaagc atcttactga ccatcggctg cattgcgcca
    24841 ttccagtcat aatggcgaat atgcggcgca ggcggtgcag tttgatcttc gtgctcaatt
    24901 gggcctggct gctgcgggat cgtcggcacc gagggcactg tggggacagg ctgttgtggc
    24961 tcggctggtt gttccggcgc tggtttcact tcttctaccg gtgcaggttc acgttgcccc
    25021 acacacccgg cgagaaacat cgccagcgcg gtaatcaagg cgtagcgact catttttgtc
    25081 atcaagattc accccttaca aatatagata aagtctgact ttgtgcgccc ccagaaaatt
    25141 ggcgctgccg tacagcgtta ccgccgaatg tgcgggaatg gtaacgctgc gtggcctttc
    25201 cagaggatgc atctccagcc ctctggcgtc ataccagtaa aaacgataat gaacggtaac
    25261 gggttcttgc ctttcgttat aaagcgttga ggatgctgaa ggttgaatat cagacgtcga
    25321 aaggacgggc ttttctgcac tgatgcccgc agccaataaa gatgactcca tcaccagcga
    25381 ttgctcatca ttcaccggaa tttccggatg tgaacgacag cccaccagca gtaacaacgc
    25441 cagagacacc agcccaaagc atccttttct catcgttaca gacctttatg cgccagcatt
    25501 ggtcccagcg gacggccacc caacaagtgc atatggatgt ggtaaacctc ttgtccgcca
    25561 tggcggttgg tgttcatgat cagacgatag ccatcttcgg caataccttc ttgctcagca
    25621 atttttgccg ctacggtgat catgcgtccc agcgcctgct catgctcagc tgagacgtcg
    25681 ttcacagtcg gaatgaggat attcggaatg atcagaatat gcgttggcgc ttgcggcgaa
    25741 atatcgcgaa acgccgttac cagatcatcc tggtagacga tatcggaggg gatctcacga
    25801 cgaataattt tgctgaatat agtttcttct gccacgacat tttccttttt caaatagcca
    25861 tgcgttgcgc catgctacgc cgcgccacac tgcgagtata gagtatggtc gagttaaacc
    25921 tgctctttca accttaactc acgatttctt aagcaaaaaa tcgttttgct gccgtatata
    25981 tctccattat tcccatttct gcctgcgaat gcgctgaaaa gacattctct gtttggttta
    26041 cacgacaaat atgaatgcgt atatttctca tttgcatttg catttgcatt tgcatttgca
    26101 agcaaaatta ttcgcacact aaatataacc gctcatcacc ctcctccggc atgagcctga
    26161 cgacaacata aaccaagaga tttcaaatgc tttcaacaca atttaacagg gataatcaat
    26221 atcaagccat caccaaaccg tcactacttg ccggttgcat agcactggca ctattacctt
    26281 ctgccgcttt tgctgcacca gccactgaag aaacggtgat tgttgagggt tcagccacag
    26341 ctccagatga tggcgaaaat gattacagcg taacgtctac ctctgcgggt accaaaatgc
    26401 agatgactca acgtgatatt cctcagtcgg tcactattgt tagccagcag cggatggaag
    26461 atcagcagtt acaaacgctg ggcgaagtga tggaaaacac gctggggatc agcaaaagtc
    26521 aggcggattc cgatcgtgct ctttattatt cccgcggatt ccagatcgat aactatatgg
    26581 ttgatggtat ccccacctat tttgaatcgc gctggaatct gggcgacgca ctttctgata
    26641 tggcactgtt tgaacgcgta gaagtagtgc gtggcgcgac aggactcatg accgggacgg
    26701 gtaatccatc tgcggcaatt aatatggttc gaaaacacgc gaccagtcgt gaatttaaag
    26761 gcgatgtctc ggcggaatac ggtagctgga acaaagaacg gtatgtggcg gatttacaaa
    26821 gcccactcac cgaagacggt aaaatccgcg cgcgaattgt cggcggctac cagaataacg
    26881 actcatggct ggaccgctac aatagtgaaa agaccttctt ctccggcatt gtcgatgctg
    26941 atttaggcga tcttacgacg ctttcagccg gttacgaata tcagcgcatt gatgttaata
    27001 gcccaacctg gggcggttta ccgcgctgga atactgatgg cagcagcaac agttacgatc
    27061 gcgcacgcag taccgcacct gactgggcgt acaacgataa agagatcaac aaggtcttta
    27121 tgaccctgaa gcagcggttt gctgatacct ggcaagcgac actgaatgcc acccactctg
    27181 aagtcgaatt tgacagcaaa atgatgtatg tcgatgccta tgtaaacaaa gcggatggta
    27241 tgctggttgg gccatacagt aattatggac ctggctttga ttatgtcggc ggcaccggtt
    27301 ggaacagtgg caaacgtaaa gttgatgcgc tggatttgtt cgctgacggt agttatgaat
    27361 tgtttggtcg tcagcacaat ctaatgtttg gtggcagtta cagcaaacaa aacaatcgtt
    27421 acttcagttc atgggccaac atcttcccgg atgaaattgg cagtttctac aactttaatg
    27481 gcaatttccc acaaaccgac tggtcaccac agagcctggc gcaggacgat accacacata
    27541 tgaaatcgtt atatgctgcc actcgtgtca cccttgccga tccgctgcat ctgatcctcg
    27601 gcgcacgtta taccaactgg cgggttgata cgctgactta cagcatggag aaaaaccaca
    27661 ccacgcctta cgctggtctg gtgtttgaca tcaatgacaa ctggtcgacc tacgccagct
    27721 atacctctat tttccagccg caaaatgatc gtgacagttc aggcaaatat ctggctccaa
    27781 tcaccggtaa caactacgag ctgggtctga aatcggactg gatgaatagc cgtctgacca
    27841 ccacgttagc catcttccgt attgagcagg ataatgtcgc tcagtccacc ggtacaccta
    27901 tccccggcag caacggcgaa accgcctata aagcggtgga tgggacagtc agtaaagggg
    27961 tggaatttga actcaacggc gcaattaccg acaactggca gctgacattt ggcgcaacgc
    28021 gctatattgc agaggataac gaaggaaacg ccgttaatcc taatctgcca cgcaccacgg
    28081 ttaaaatgtt caccagctat cggttgcctg tcatgccaga gttgacagtc ggcggtggtg
    28141 ttaactggca aaatcgcgtg tataccgaca ccgtgacacc gtatggcacc ttccgcgccg
    28201 agcaaggtag ctacgcgttg gtggatctct tcacccgcta ccaggtgacg aaaaacttct
    28261 cgttacaggg gaacgtcaat aacctgttcg acaaaaccta cgataccaac gtggaaggtt
    28321 ctatcgtcta cggcgcaccg cgtaatttca gcattaccgg cacgtatcaa ttctgaataa
    28381 cacctgtaaa aaaggcagcc atctggctgc cttagtctcc ccaacgtctt acggattagt
    28441 ggttacggat gtactcatcc atctcggttt tcaggttatc ggatttagta ccgaaaatcg
    28501 cctgaacacc agaaccagca accactacgc ccgctgcgcc cagtttcttc aggccagcct
    28561 gatccacttt agacacatca gcaacgctga cgcgcagacg ggtaatacat gcgtcgaggt
    28621 tagtaatgtt ttctttacca ccaaatgcag caaccagagc cggtgccatt tcgctggtac
    28681 ctgtcgcttt tgcatcttca gtcgcgtctt cacgacccgg cgttttcaga tccagtgctt
    28741 taatcagcac gcggaagatg gtgtagtaaa caatcgcata accgataccg acgatcggga
    28801 acagccacag tttgctgctg ttaccagaca gaacgatgaa gtcgatcaga ccgtgcgaaa
    28861 acgacgtacc gtcacgcatc cccagaagaa tacagattgg gaatgccagg cctgccagaa
    28921 tcgcgtggat gatgtacagg atcggcgcaa cgaacatgaa ggagaactcg atcggctcgg
    28981 tgataccggt caggaacgag gtcagcgccg cggagatcat aataccgccc actttcgcgc
    29041 ggttttctgg tttagcagag tgccagatag caattgcggc agctggcaga ccgtacattt
    29101 tgaacaggaa gccaccagac agtttacccg cagtcgggtc acccgccata taacgcggaa
    29161 tgtcgccgtg gaaaacctga cctgctgcgt tggtgtattc accaatctgc atctggaaag
    29221 gtacgttcca gatgtggtgc agaccaaacg gtaccaggca acgttcgatg aaaccgtaaa
    29281 tgccaaacgc aactaccggg ttctggtaag cagcccactg agagaaggtc tggattgcag
    29341 aaccaatcgg cggccaaatg aaggacagca caacgccagt aaagatggca gccaggccag
    29401 aaatgatcgg cacaaagcgt ttaccggcaa agaagccaag atactcaggc agcttaatac
    29461 ggtagaaacg gttaaacatg tacgctgcga tcgcaccgga gataatccct ccgagtacgc
    29521 cagtatccgc caggtgttta gaggcgattt cttcagcagg taaatgcagt accagtggcg
    29581 caaccacggc catggtttta accatgatgc cataggcaac aactgcagcc agcgcggata
    29641 cgccatcgtt attggtaaag ccgagggcga caccgatcgc aaaaatcagt ggcatgtttg
    29701 caaagacgga accgcctgct tctgccataa catgcgatac aacggcgggc agccagctga
    29761 aattcgcgga accgacgccc agcagaatac ctgcgatagg cagtacggat accggcagca
    29821 tcagcgattt accgaccttt tgcaggttag caaatgcatt cttaaacata attgagagtg
    29881 ctcctgagta tgggtgcttt ttttacgttc tcacgcgtgg caagggggga gagcctcgcc
    29941 gtgtacaggg catctaagcg ccctttattt attacacaga gtaaaataat tcagtgccaa
    30001 tttgtttgac ggctatcacg tttcaacttc agatcattgg agaagttgca cattttgaca
    30061 aaacatatgt cacaatattt caaacgtggt tattcaccgc ccaattttcg gcggtgaact
    30121 ttactcgttt tagtcattaa ttacgagctt taaaaaaact cattcaacgg atggattgaa
    30181 ggcgggaagc gtcgatgtga aacagacggg cgaagttatc ggtggttacc tgcgccagtt
    30241 cttcaacggc aacacctttc aacacagcca tgtattctgc aacgtcacga accatcgcag
    30301 gttgattctc ttttcctcga tgcggcaccg gcgcaaggta aggtgagtca gtttccacca
    30361 gtaaccgatc cagggggaca taacgcgcag catcgcgcag ttgctccgca ttacggaagg
    30421 tcacaatgcc ggaaaaggag atgtaaaatc cgagatccag taatttgccc gccgtttctc
    30481 tgtcctctgt aaaacagtgt agtacgccac cgcaatccgt cactttttct tcgcgcagaa
    30541 ttgccagcgt atcggcgcgg gcgtcacggg tatggacgat aaccggcttg ttcagttcac
    30601 gaccaatctg gatatgatgg atgaaggact cttgctgacg tactttagtt tccggtgtgt
    30661 aataataatc cagcccggtt tcacccagcg ctacaacacc ctcttctgcc gccagacggc
    30721 gtaaatcttc tacatcgtag ggatcattct ggttgagtgg atgcacgcca caagaaaata
    30781 cgacgttgtc acgttcgcct accagatccc gcatgtgtaa ataacccggt aatgttgtgg
    30841 cgactgccag acaaaatttc acatcgcgtg cggcggcttt cgccagaacg tcatccacgt
    30901 ccttatgcaa agattcataa tccagaccat cgagatggca gtgtgagtcg actaaaaaca
    30961 tgatgtctct cttaaagatg aggaaccggt agcacaacgc ccggttgcag gtaatgctca
    31021 atacgcagta aaagatcggt gatgagaagc tcgcggttga tgcctgtgac agacattaac
    31081 tgttcacgaa tgtggcaaac atcccccagt atagcctgca ggcgcgaggg agaaagatgg
    31141 tttgccagtt cggcgaccag gcccggcaca tcaacattgg tcacctgcgc agcgccatga
    31201 tggcgtttta gcgcatccat cagcaacgtt gccagccagt gtaaacgcgc cggagcttgt
    31261 tcatgattaa gggccgccag cagcgaatac cagtcgcccg atggcacgct atatgccaac
    31321 gcctgacaca atgtttcacg agcctgccag ttatctccct gaaacaacgc cagtgccgcg
    31381 ccaggcgaac cggcgcttaa gcgcaatgcg gcaagtaatg catcctgtga cattgtcact
    31441 tcgcgtgaaa gccaggtcac ggcgtactgt tccggcggcg gcgcaaggta atgtaaccga
    31501 caacgactac gtaacgttgc cagtaaacgt tcaggctcgc gggtagccag gaaaaaccag
    31561 gtttctgctg gtggctcttc aagcgttttc agcaatgcgt tagccgcggc gtcggtcagt
    31621 aaggcagcat cggttaccca gacaactttc gcaccaccta agcgtgcgtg ctcattcagc
    31681 ttttcggtga cctcacgtac cgcatcaatg cccagcgtat ttttcccttt ttcgggagcc
    31741 agggtgtagt aatcgggatg cgtgccagcc tgcatcaact gacatccacg acagtgaccg
    31801 caacttttgt ggccctgcgg ttgttggcag agtaaataac ggctcagggc gtagattaaa
    31861 gcatcatcgc ccatgcccgg taacgcctga atgagtagcg catggtgacc tcttccggcc
    31921 tgatagctgg ctaccagttt ttcgaaatca ggtcgtaacc atggatacca tctcatgcgt
    31981 ccaactcctt cacccagcgg gtcacggtag tgcggattgc atccatcacg gcctccagcg
    32041 gctgggtggc atcaatggta tgaatacttt tatcttgtgc agccagttcc agatagcggg
    32101 cgcgggtgcg attaaagaaa tcgaaagact cttgctcaat acgatccagc tcgccgcgcg
    32161 cacgcgcgcg ttttaagcca acttccgggg taacatcgag atagagcgtt aagtcggggc
    32221 gaaaatcccc gagaacagca tcacgcagtg ttgccagcat atgttggtca ataccacgtc
    32281 ctccgccctg atacgcctga gtggagagat cgtggcgatc gccaataacc caggtgccgt
    32341 tagccagcgc aggtttgatg accgtttcta ccagttgaac gcgcgcggca taaaacatca
    32401 gaacttcggc tttatcggta atgatttcat cgcctaccga tttgatatcc agcaccaggc
    32461 ttcttaactt ttcggcaagt tgcgtaccac caggttcccg agtgaaaacc atgtcgcgga
    32521 tacccagttg ctcgagcgtc tcaaccacca cattacgcgc ggtagttttg cctgcgcctt
    32581 ccagcccctc aatgacgata tacttactgc gcattttttt ccttaagcac tttcagataa
    32641 tcctgcacag acttgttatg actggcaaga ttggtattaa acgtgtgacc acctttacca
    32701 tcggccacaa aatagagata cggcgttttt gccggatgcg cagcagcctt cagcgaatcc
    32761 gcccccggcg tagctatcgc acccggcggc aaaccggtaa tggtataggt gttatacgct
    32821 gtcggcgttt ccaggtctgc acgagaaagt ttgccattat aacgctctcc catcccgtaa
    32881 atcacggttg ggtcggtctg caggcgcata ccaatgcgta aacggttgat aaataccgag
    32941 gcaacctgat cgcgttcact ggcaatggcg gtttcttttt cgataattga tgccatcgtc
    33001 accagctgat ttttatcttt ataaggcaga ccgtccgcac gcccttccca ggcgctatcg
    33061 accgctttca ccattttctt gtgcgctcgc ttgagtaacg cgacatcggt ggtattggcg
    33121 gtatacatcc aggtgtctgg ccagaaccaa ccttcaatcc actccgggtt ttccagttca
    33181 agtgcctgcg ctacggtgat gtacttatca tcgctcagcg tgtgcttgat atacggggcc
    33241 tcacgcaatt gcttgaggta gtcgctcaga cgcatccctt ctaccagtcg cagagggaac
    33301 tgtgcttctt taccgctttc caacaatttc agcatctcgc gcacggtcat ctgcggtgta
    33361 aagcggtaag tcccggcttt aaagtgagaa agatccggtt cgatacgcag caaccattga
    33421 aaaacccgtg ggcgattgat gatcttatcg gcataaagct gttcaccaag cgccagacgt
    33481 ccggtccctg gcttcagggt aaatatcgtc tcttctttga taagcaattt gctgtcggca
    33541 agatagcgaa ccttccagac gcccacacca gcggcgatac ccagtaccac cagcaataac
    33601 aagattatca ataacacttt tttcatgact aattcgggcg ctcacaaagt ggggctaaat
    33661 attcatataa cgttgctgac gaaaaggaga catcgccaca ggcacgtacg ggcatcactg
    33721 gcattaacgc attacaaata accatctcat ctgcctgcaa cgcctcttcc agagaggctt
    33781 gcacttcgac aagctgataa gaggattgtg ccagcaaacg gatacagaat tgtcgcataa
    33841 tgccgttaac acctgcctga tccagtcgcg gcgtataaac tacgttgccc ttccgccaga
    33901 acaaattagc tgcacagcat tccgtaaccc ccccttcgct gtcaaggacc agcgcctcat
    33961 cagcgtttgt ctgctcaaga tgagagcgga tcaatacttg ctcaagccga ttaagatgtt
    34021 taatacctgc aagatgagga ttgcgcccca gccgcaccgg gcttagcgcc aacgtgatcc
    34081 cttcgttacg caaacggtcg taatgtgcag gataagccgt aacggagaga atccgcgttg
    34141 ccggtccact gttcaatgtg ctgtaccctc gcccgccact accgcgactg atcacgactt
    34201 tcagtacacc attttgctgt tctgctgcca gcgttttcat ctcttgttca agctgaggcc
    34261 agaagtcaca ggaaatcatc aaccgctgac aagcatcctg tagtcgctgg atatgcgccg
    34321 ataacaaact gactttaccg tcgataactc tggcggtggt aaaacaacca tcaccaaact
    34381 gcgttgcccg atcgcttact gacagcgatt cctgcttata accgttaatt aagaacatac
    34441 cggctcctta tcagagaatt gttagtgtgg cagcatgttc actacggaac aagtcggaat
    34501 aaaagctaag aaaaaaggcc cgcaagcgga ccttttatat gggtggaaaa tgacaactta
    34561 gatcttttta aagatcaaag aaccattagt gccaccgaag ccgaaggagt tacacagcgt
    34621 gtattccatt ccgctgacct gacgtgcttc atgcggtacg aaatccagat cgcaaccttc
    34681 atccgggtta tccaggttga tggttggcgg aacagcctga tcgcgcagcg ccaggatgga
    34741 gtagatagat tctactgcac ccgccgcacc taacaggtga ccggtcatag atttcgtgga
    34801 gcttaccaac acacggcttg cagcttcacc gaagatggtt ttcaccgcct gcgcttcagc
    34861 tttatcgcca gccggcgtag aagtaccgtg cgcgttaacg tagccaatct gactcgcttc
    34921 aatgcctgca tcacgcagag catttgccat tgccagagct gcgcctgcgc cattttctgg
    34981 cggtgacgtc atatgataag catcgctgct cataccaaag ccgacgagtt cagcgtaaat
    35041 tttcgcaccg cgttttttcg cgtgttcgta ctcttcaagt accagcatac cggcaccatc
    35101 gcccagtacg aaaccatcac gctctttatc ccacgggcgg ctcgccgctt gcgggttatc
    35161 attgcgggta gataatgcac gtgccgcgcc aaaaccacca acacccagcg gcgtactggc
    35221 tttctctgcg ccacctgcaa ccatcacgtc agcatcgcca tacgcgataa tacgcgcagc
    35281 atggccaatg ttgtgcacgc cggaagtaca ggcggtcgcg atagagatgc ttgggccacg
    35341 caggccatac atgatagtca gatgacctgc caccatgttc acaatcgttg acggaacgaa
    35401 gaatgggctg atcttacgtg gaccaccgtt catcagagat gtgtggtttt cttcgatcag
    35461 tccgaggccg ccaatcccgg agccaattgc ggcaccaatg cgggttgcgt tctcttccgt
    35521 tatttcaagg ccagaatcct gcatggcctg aacgccagcg acaattccat attgaatgaa
    35581 ggcatccatc ttgcgctgtt ctttgcgcga gataatgtcc tcacagttaa aatcctttac
    35641 taagccagca aatttcgttg cataggcgct agtatcgaaa tggtcgatta ggctgatgcc
    35701 actctgaccg gcaagcagag ctttccaggt agactctacg gtattgccga caggagacaa
    35761 catgcccagt ccggtcacaa ctacacgacg cttagacacg tttgtcctcc agggagggaa
    35821 aaaatgattc tagtgggaca aaaagataaa actcaggcgg tcgaacgacc gcctggagat
    35881 gttcacttac gcctggtggc cgttgatgta atcaatggca gcctgaacgg tggtgatttt
    35941 ctcagcttct tcgtccggaa tctcagtatc aaactcttct tccagagcca ttaccagctc
    36001 aacggtgtca agagaatccg cgcctaggtc ttcaacgaaa gaagcattgt tggtaacttc
    36061 ttcctgctta acgcccagct gttcgccgat aattttctta acgcgttctt cgatagtgct
    36121 catactctta aatttcctat caaaactcgc tttcgcgatg gttttcgtag tgtataaaat
    36181 gttgaaaaat ttgcaactaa atcccggcag gtcttaccac gattttacgt tattttgagg
    36241 ccttaccccc caataacgca aatcattttg cactaattat tactcataac cacgcagact
    36301 gcgcgcaaca tgagcatttt gtgcaaatcg cggtcagacc atgtacatcc cgccgttcac
    36361 atgcaaagtt tcacccgtga tgtaagctgc ttcgtcggat gccaggaatg caaccgcgtt
    36421 ggcgatttcc tgtgcgccgc cgaggcgacc cgcaggaacc tgcaccagga tacccgcacg
    36481 ctggtcatcg ctcagcgcac gtgtcatgtc cgtttcaata aagcccggag caacaacgtt
    36541 tacagtaata ccgcgtgacg caacttcgcg cgccagtgat ttactgaagc cgatcaagcc
    36601 cgctttcgcc gcagcgtagt tggcctgacc gccatttccc atggtaccaa ccacagaacc
    36661 gatagtgata atacgaccat gacgcttttt catcatagcg cgcattaccg cttttgacag
    36721 acggaaaaca gatgaaaggt tggtttcgat aatatcgttc cactcttcat ctttcattcg
    36781 cattaacagg ttatcacgag tgataccggc attattgacc aggatatcca cttcaccaaa
    36841 ttctgcgcga attttttcca gaacagattc gatagatgcc gggtcggtca cattcaacat
    36901 cagacctttg ccgttagcac ctaaataatc actgatcgcc tgagcgccat tttcactggt
    36961 cgcagtgcca ataactttcg cgccacgggc tgcgagcgtt tcagcaattg cgcggccaat
    37021 tccgcggctt gcaccggtta ccagtgcgat ttttccttca aaattcatga tgttcctctt
    37081 ttaaagctcg agcgccgctg ccatcgctga aggttcgttc agcgccgagg cggtcagggt
    37141 gtcgacaatg cgtttcgtca ggccagtaag cactttgccc gggccgactt catagagatg
    37201 ttctacgcct tgcgctgcca tgtactcaac agacttcgtc cactgaaccg ggttatacaa
    37261 ctgacgtacc agtgcgtcac gaatggcatc accattggtt tcgcatttca catcaacgtt
    37321 attcacaaca ggaactgttg gtgcgttaaa ggtgattttc gctaattcta ctgccagttt
    37381 gtcggctgct ggtttcatca gcgcacagtg agacggtacg ctcactggta acggcagagc
    37441 gcgttttgcg cccgcagctt tacaggcagc gccagcacgc tcaaccgctt ctttatgacc
    37501 ggcaataacc acctgtcccg gagagttaaa gtttaccgga gaaacgacct gaccttctgc
    37561 agcttcttca cacgcttttc caatagacgc atcatccaga ccgatgattg ccgccatagc
    37621 gcccgttcct tccggtacgg cttcttgcat gaacttgccg cgcatctcaa ccagacgcac
    37681 cgcatcagcg aaatcaatca caccagcgca aaccagcgcg gagtattccc ccaggctgtg
    37741 accggccatc attgccggtg ctttaccgcc atgctgctgc catacgcggt acagcgcaac
    37801 agaggcagtc aacagcgctg gctgggtttg ccaggtttta ttcagttctt cagctggccc
    37861 ctgctgggtc agcgcccaca ggtcgtagcc cagcgccgca gaagcttcag caaacgtttc
    37921 ttcgacaatt ggatagctcg ccgccatatc agccagcatt ccaacggttt gagaaccctg
    37981 tccagggaac acaaatgcaa attgcgtcat gttttaatcc ttatcctaga aacgaaccag
    38041 cgcggagccc caggtgaatc caccgccaaa ggcttcaagc agaaccaact gccccggctt
    38101 gatgcgcccg tcgcgtacag cttcatccag cgcgcacggg acagaggccg cagaggtatt
    38161 accgtggcga tccagcgtca ccacgacatt gtccatagac ataccgagtt ttttcgccgt
    38221 tgcactgata atacgcaggt tagcctgatg cggaaccagc cagtccagtt gagaacggtc
    38281 aagattattc gccgccagcg tctcatcaac gatgtgcgcc agttccgtta ccgcaacctt
    38341 gaagacttcg ttgcccgcca tcgtcagatg aattgaattc tctggattca cgcggtcggc
    38401 gtttggcagc gtcagcaatt caccataact accgtcggca tgcagatggg tggaaatgat
    38461 tcccggctct tcagaggcag ccagcaccgc agcgcccgcg ccatcgccaa aaataataat
    38521 agtcccacga tctgttggat cgcaggtgcg cgccagtaca tcggaaccga cgaccagagc
    38581 atacttcacc gccccagatt tcacgtattg atcggctacg cttaatgcat aggtgaaacc
    38641 tgcgcaggct gctgcaacgt caaatgccgg gcaaccttta atgcccaaca tgctttgaat
    38701 ctgacaagct gcgctcggga aagcgtgcgt agcagaagtc gttgccacaa cgatcaggcc
    38761 aatctggtct ttctcaatgc ccgccatctc aattgcgcgt gtcgccgctt caaagcccat
    38821 ggttgaaacg gtttcgtttg gcgcggcaat gtggcgttcg cggataccgg tacgagtgac
    38881 aatccactcg tcagaggtgt ccaccatttt ttccaaatcg gcgtttgtcc gcacttgttc
    38941 gggcagatag ctgccagtac caataatctt cgtatacatg tacgctcagt cacttttcgg
    39001 ttatataccg tcacttgcaa actgcgagtt cgctggcagc gtcctgctac cgcagagttc
    39061 cgcttttgcc accgtccagc agctcaaaac cagctgggta tacagattcc aggcgagcgg
    39121 caattcgctg aggaacttgt cgctgcaccg cctgcactgc ctgttcaatc gcgaccgcaa
    39181 aagctcgctg attggctgca ccatgacttt ttatcaccgt gccgcgcaat cctaacagac
    39241 aggcgccgtt atactggtcg gggttgaggt gactgaatcg cctcgtcagg ctcttttgta
    39301 gccaacgctt taataacagt agccaccacg accgtttttt cccttcaccc tgagatttca
    39361 gcagagaaag gaacatcctg acaacacctt ccatcgtctt taatgtgaca tttcctgtaa
    39421 agccgtcaca aaccagcaca tctgtcttgc cagttaacaa ctcattggct tcaagatagc
    39481 cgatataatt gatagaaggg attgttttaa gcaccgctga ggcatcccga atactatcga
    39541 gaccctttac ttcttcttca ccaatattga gcaacgccac gcgaggattg ggaatttcca
    39601 ccacctcttc agccaggact gagcccataa tggcaaattg caccaacatt gtgctgtcac
    39661 aatcgacgtt ggcccctaag tcaaggacca ccgttttgcc cttttgctga tgtggtaata
    39721 ccgtcaccag cgccggacgc tcaatcccct ccaggggctt gagtaataat tttgccagcc
    39781 ccatcagtgc cccggtatta ccggcactga cacaggcttg cgctcgacct tctttcacca
    39841 gctccagggc cacgcgcatt gaactcccac gactggcgcg gatagcttgc gaaggccggg
    39901 catcactggc gataactgac tgcgcaggaa taatctgcag acgcgaacgt tgttcaaagt
    39961 cagctttagc aagtaatggc gtgatggcgt cgggattgcc gactaaaaga agagtgagtt
    40021 gcgaattaga attcagtgcc tgcaatgctg caggcactgt cacggaaggg ccaaaatccc
    40081 ctcccatgac atctaacgcc agggttagac gtgtcaaggt atcgtcgccg cccggtttgg
    40141 tctttcccca gttgcctggg gaaaatcctc actaagcttc atcacgcaga cgcgtgatta
    40201 cttagcgatg accttgcggc cgcggtagta accgtcggca gtgatgtggt gacgcaggtg
    40261 tttttcacca gaagttttgt ctacagacag gctggtgact gcggtcagcg cgtcatggga
    40321 acgacgcatg ccacgtttgg aacgggttgg tttattctgt tgtacggcca tggaccttac
    40381 tcctcaatta cggtttatcc ccgatccaac ggggagcacc aattacttac gctttaagct
    40441 ggctaatacg gcaaatgggt ttggcttttg cgcttcttca ggcagttcac caaagaccat
    40501 gtccgcttcg gacacttcac agtgttcaga atcatgcacc ggaactaccg gcaaggcgag
    40561 gatgatttca tcttcaacca ttgcaagcag atcgatttca ccgaattcgt taacctcaat
    40621 cggttcatac gcttccggca gtgcttcagc ctgttcgtct gaacgcacag gactaaaaca
    40681 atacgttgtg tagacctgat gagtaaacgg cttcccgcaa cgctgacact cgagcgttac
    40741 cgtcaccttc gcatcgccgt ttaacactgc gagacgttgg ttatcgatag cgaacgacat
    40801 ggagcattcc acatcactgt ccacactgac tacggattcg gcgacgcgct caacctgatc
    40861 aggagtatag ataccctggt aatcaaggcg tttttgagcc gtacgaaccg gatcgagagt
    40921 caggggtaat tttacctttt gcatagggcg cgcatattaa ctttgtaacg tcatagagtc
    40981 aaagaaaaag gcagcctgtg gttgcctttt gccaataatt cgcacacatt gcgggttact
    41041 gctttatttt cgttcagtgc tgataacgaa catcaacctg caaaacgaag cgcatagttt
    41101 aaaatggcta tcatcaatac gctatatctg atggaaaaaa atatgcctaa acttatttta
    41161 gcctccacct cgccctggcg tcgcgctctt ctggaaaaac tgcaaatctc ttttgaatgt
    41221 gcagcacccg aggtcgacga aactccacac agcgacgaat caccgcgaca gttggtgctt
    41281 cgactggcac aagaaaaagc gcaatctctg gcgtcacgtt atccggatca tttaattatt
    41341 ggttcagacc aggtatgtgt tcttgatggc gaaatcaccg gtaaaccgtt aacggaagaa
    41401 aatgcccgtc tgcaattacg caaagccagc ggcaatatcg tcactttcta tactggactg
    41461 gcgttgttta attcggcgaa tgggcatctg caaacagaag tggagccttt tgacgtccat
    41521 ttccgtcacc tgagcgaggc ggagattgat aattacgtgc gtaaagagca tcccctgcac
    41581 tgcgcgggta gctttaagag tgaaggattt ggcattactc tgtttgagcg tttagagggg
    41641 cgtgatccta acacgctggt tggtttaccg ctaatcgcgc tgtgtcagat gttacgtcgg
    41701 gaagggaaaa acccgctgat gggatagttt tttgcgccgg atgcggttac gtcgcatccg
    41761 gcaatcggtg ctggcggcct gatgcggcgt aaacgcctta tcccattatc atgcttatat
    41821 gctcgtatta gcgcgcgtta cgcagctttt gcaaacaacg cttcaaacca tcatccatcg
    41881 gcgcttcgat acgcatcacc tcaccggtcc ccggatgggt aaacttcaac gctgcggcgt
    41941 gcaggaacag acgatttaat cccgtgcctg cttcagtgag ctgtctgtca aattcacggt
    42001 caccgtagcg atcgtcaaag gcaatcggat gacctgcata ctgtgtatgc acacggatct
    42061 gatgagtgcg ccctgttacc ggactacaac gcaccagggt ggcaaatgca tagcgttctt
    42121 ccactttaaa gcgtgtttcc gacggtttgc cttcctgact cacacgcacg atacgttcgc
    42181 cgctttgcag aatatttttc aataacggcg cctgaacgct cttcacatgc gactgccact
    42241 gaccgcgcac cagcgccagg taatcttttt gcatcccttt ttcacgtaat tgctcatgca
    42301 gagaacgcaa cgccgagcgt tttttcgcta ccagcaacac acctgaggtg tcccggtcaa
    42361 gacgatgaac cagttcaagg aaccgcgctt ccgggcgcaa cgcccgcaaa ccttcaataa
    42421 cgccgaagct taaaccactg ccgccatgta ccgccgtacc ggaaggttta ttcagcacca
    42481 ggatgtgatc atcttcatat aagatgacgt ccgccagcgc cgccaccttt tgcagatgtg
    42541 gcgaaaccgc ctcttcttcc cgctcagcaa cgcgaaccgg tggaatacgc acctcatcac
    42601 ccgcttcgag tttatattca ggcttaatac gttttttgtt cacccgcact tcgcctttac
    42661 gcaaaatacg gtaaatcata ctttttggta cgcctttcaa ttgggtacgc aaaaagttgt
    42721 cgatacgttg ccccgcttcg tcggcggtga tagcaacaat ttttacggat ggagtctctg
    42781 ttttcatggt gggcgattct aattagccaa caggattcgc gccactcatt tttctatgct
    42841 tatatttagt tttgcacctt attacttcac tacgtgatca ctttattgat ggttattaaa
    42901 ccaatcacca gcaagaagtg aaaaaactgt gagtaagcgg gtgataaatg gtaaaagtca
    42961 tcttgctata acaaggcttg cagtggaata atgaggccgt ttccgtgtcc atccttgtta
    43021 aaacaagaaa ttttacggaa taacccattt tgcccgaccg atcatccacg cagcaatggc
    43081 gtaagacgta ttgatctttc aggcagttag cgggctgcgg gttgcagtcc ttaccggtag
    43141 atggaaatat ttctggagag taatacccag tctgtttctt tgataattgc gctgtttttc
    43201 cgcatgaaaa acgggcaacc gacactctgc gcctctttga gctgacgata accgtgaggt
    43261 tggcgacgcg actagacacg aggccatcgg ttcacacccg gaaaggcgtt actttgcccg
    43321 cagcttagtc gtcaatgtaa gaataatgag taagttacga tgaaaagaat gttaatcaac
    43381 gcaactcagc aggaagagtt gcgcgttgcc cttgtagatg ggcagcgtct gtatgacctg
    43441 gatatcgaaa gtccagggca cgagcagaaa aaggcaaaca tctacaaagg taaaatcacc
    43501 cgcattgaac cgagtctgga agctgctttt gttgattacg gcgctgaacg tcacggtttc
    43561 ctcccactaa aagaaattgc ccgcgaatat ttccctgcta actacagtgc tcatggtcgt
    43621 cccaacatta aagatgtgtt gcgtgaaggt caggaagtca ttgttcagat cgataaagaa
    43681 gagcgcggca acaaaggcgc ggcattaacc acctttatca gtctggcggg tagctatctg
    43741 gttctgatgc cgaacaaccc gcgcgcgggt ggcatttctc gccgtatcga aggcgacgac
    43801 cgtaccgaat taaaagaagc actggcaagc cttgaactgc cggaaggcat ggggcttatc
    43861 gtgcgcaccg ctggcgtcgg caaatctgct gaggcgctgc aatgggattt aagcttccgt
    43921 ctgaaacact gggaagccat caaaaaagcc gctgaaagcc gcccggcccc gttcctgatt
    43981 catcaggaga gcaacgtaat cgttcgcgca ttccgcgatt acttacgtca ggacatcggc
    44041 gaaatcctta tcgataaccc gaaagtgctc gaactggcac gtcagcatat cgctgcatta
    44101 ggtcgcccgg atttcagcag caaaatcaaa ctgtacaccg gcgagatccc gctgttcagc
    44161 cactaccaga tcgagtcgca gatcgagtcc gccttccagc gtgaagtccg tctgccatcc
    44221 ggtggttcga ttgttatcga cagcaccgaa gcgttaacgg ccatcgacat caactccgca
    44281 cgcgcgaccc gcggcggcga tatcgaagaa accgcgttta acactaacct cgaagctgcc
    44341 gatgagattg ctcgtcagct gcgcctgcgt gacctcggcg gcctgattgt tatcgacttc
    44401 atcgacatga cgccagtacg ccaccagcgt gcggtagaaa accgtctgcg tgaagcggtg
    44461 cgtcaggacc gtgcgcgtat tcaaatcagc catatttctc gctttggcct gctggaaatg
    44521 tcccgccagc gcctgagccc atcactgggt gaatccagtc atcacgtttg tccgcgttgt
    44581 tctggtactg gcaccgtgcg tgacaacgaa tcgctgtcgc tctctattct gcgtctgatc
    44641 gaagaagaag cgctgaaaga gaacacccag gaagttcacg ccattgttcc tgtgccaatc
    44701 gcttcttacc tgctgaatga aaaacgttct gcggtaaatg ccattgaaac tcgtcaggac
    44761 ggtgtgcgct gtgtaattgt gccaaacgat cagatggaaa ccccgcacta ccacgtgctg
    44821 cgcgtgcgta aaggggaaga aaccccaacc ttaagctaca tgctgccgaa gctgcatgaa
    44881 gaagcgatgg cgctgccgtc tgaagaagag ttcgctgaac gtaagcgtcc ggaacaacct
    44941 gcgctggcaa cctttgccat gccggatgtg ccgccagcgc caacgccagc tgaacctgcc
    45001 gcgcctgttg tagccccagc accgaaagct gcaccggcaa caccagcaac tcctgcacaa
    45061 cctgggctgt tgagccgctt cttcggcgca ctgaaagcgc tgttcagcgg tggtgaagaa
    45121 accaaaccga ccgagcaacc agcaccgaaa gcagaagcga aaccggaacg tcaacaggat
    45181 cgtcgcaagc ctcgtcagaa caaccgccgt gaccgtaatg agcgccgcga cacccgtagt
    45241 gaacgtactg aaggcagcga taatcgcgaa gaaaaccgtc gtaatcgtcg ccaagcacag
    45301 cagcagactg ccgagacgcg tgagggccgt cagcaggctg aggtaacgga aaaagcgcgt
    45361 accgccgacg agcagcaagc gccgcgtcgt gaacgtagcc gccgccgtaa cgatgataaa
    45421 cgtcaggcgc aacaagaagc gaaggcgctg aatgttgaag agcaatctgt tcaggaaacc
    45481 gaacaggaag aacgtgtacg tccggttcag ccgcgtcgta aacagcgtca gctcaatcag
    45541 aaagtgcgtt acgagcaaag cgtagccgaa gaaacggtag tcgcaccggt agctgaagaa
    45601 actgtcgctg ccgaaccaat tgttcaggaa gcgccagctc cacgcacaga actggtgaaa
    45661 gtcccgctgc cagtcgtagc gcaaactgca ccagaacagc aagaagagaa caatgctgat
    45721 aaccgtgaca acggtggcat gccgcgtcgt tctcgccgct cgcctcgtca cctgcgcgtg
    45781 agtggtcagc gtcgtcgtcg ctatcgtgac gagcgttatc caacccaatc gccaatgccg
    45841 ttgaccgtag cgtgcgcgtc tccggaactg gcctctggca aagtctggat ccgctatccg
    45901 attgtacgtc cacaagatgt acaggttgaa gagcagcgcg aacaggaaga agtgcaagtg
    45961 cagccgatgg tgactgaggt ccctgtcgcc gccgctgtcg aaccggttgt tagcgcgcca
    46021 gttgttgaag aaatggctga agtcgtagaa gcccccgtac cggttgccga accgcaaccg
    46081 gaagtggttg aaacgacgca tcctgaagtg attgccgccg cggtaactga acagccgcag
    46141 gtgattaccg agtctgatgt tgccgtagcc caggaagttg cagaacacgc agaaccggtg
    46201 gttgaaccgc aggaagagac ggcagatatt gaagaagttg ccgaaactgc tgaggttgtg
    46261 gttgctgaac ctgaagttgt tgctcaacct gccgcgccag tcgtcgctga agtcgcagca
    46321 gaagttgaaa cggtaaccgc ggttaaacca gagatcaccg ttgagcataa ccacgctacc
    46381 gcgccaatga cgcgcgctcc ggcaccggaa tatgttccgg aggcaccgcg tcacagtgac
    46441 tggcagcgcc ctacttttgc cttcgaaggt aaaggtgccg caggtggtca tacggcaacg
    46501 catcatgcct ctgccgctcc tgcgcgtccg caacctgttg agtaataatt agctcaagta
    46561 atcaagccct ggtaactgcc agggcttttt tatttcatct ttgaatcaac atattaaaat
    46621 tcatcgaatg gcatccttgc taaccaacaa tgcaaaatag gcaaaaaaat gcccgccccg
    46681 gcgatccctg agcgggcaga caaaacatac ccagtttcat gatatgtttt aaagcgagct
    46741 tatttgttga gctggaagag cgacaatccc tgcatatcgg taaatgcttt atacgatgcc
    46801 tgcaatgccg tttgctgcat gatgtaagat gaaatagttg cattccagtc cacatcaacc
    46861 agatcgctca tctgctgcgt ttgccctaat gcgcgatcgc tacctaatga atccagcgac
    46921 tccagttcgt tcagctgcgt gcctaattcc gcgcgaacgg tcaggacatt gttcagcgag
    46981 tttttcagtc cgcggttggt tttatctaac gccgcagcgg cggtttcttt atccgcttcg
    47041 ctatccgcga ccggcgtttt cagggctgcg atggcgctat ccagcatggc aaaaagattg
    47101 gtttcagaag cgctaccgtc tggttccgct accgcgttac tggtaatact gtcgaaaatt
    47161 ttgtcgcccg tatgtcctat caccatcgaa cgcgaagcat cgacctgttg cttaatactt
    47221 tctgttccgc cgacataatc accgttaacc tcactaaacg gcgcagtctc tgttttataa
    47281 ccggcaaaaa tgtagcgccc gttaccgtca gtggtgttcg ccagattcag caactggtca
    47341 cgaagcccct gaatatccgt agccagcgag gcccggtcat tgtcactcaa ggtgccattg
    47401 ctggcgtaga caattttttc ctgagcattc tggatagcgg tggtgacctg gctaagtaca
    47461 ctctcttcca gtgacacttt ttgagtggcg aaagtacgtg ccagcgtgta ctggctgttt
    47521 tgcgcctgtg cctgggagag aacaacagct tgtgatgcag caatgggatc gtctgaaggg
    47581 ttaacgactc gcttacccgt cgacatctgc tcgccgtact tcatccattc tgcctgagaa
    47641 ttggtgatgc cgcgcatgtt ttgctggtac atcatctggg tactgaaacg cattttatct
    47701 ccccttagcg aatgttaatc agcgcatcaa aaatcgcgtt tgccgtctgc agaacctgcg
    47761 cattcgccag gtaatactgc tgaaaacgtt gcagatttcc gtactcctca tcgagattga
    47821 caccggaaat cgactgctgc tgattggaaa gctgcgtcac cacattacct tgcgtggtgc
    47881 tactggtttt caacgtcgcg gttttattac cgatatcact cactaacgag gcataagcgt
    47941 cgttaaagga tttcgctccg cccaccgttt tactgttgct ttgcagatcc agcagggcct
    48001 gaccgttgcg gttgtcgcta tcacccgcat cttcttcgct cgccatcgct attttcgctt
    48061 cgtcggtgat taatacatcc atgttgacga tggcgtcact tactggtttc agcgtgaagc
    48121 tgtcgttaac ggcaggcgtt cctgtaaacg tcaactccag accatcaaat gccactttac
    48181 cgttggcatc tggcgtcacc gtaaaagtgg tattgctggc aaggcgggtg acctgccact
    48241 gattattatc gaacgagatt ttgtaatctg tcgccagtac cgcggaggca tcagttaccg
    48301 tggcaccgat cgcaacgtta ccgttgtttt tagtgttttg cagaaccgcg ggcttaccga
    48361 tagcaaagaa atcttcaccc tcatcgccgt tggcatcaaa tccggctttg tgttggctgt
    48421 tgaaagcctc agcaaatgcc agcgccagtt gtccaagcgt attacgcgtc tggtccagat
    48481 cctgagaacg gaatgtcaga atgccgccca gcgacccggt attcagtaat ttctccggga
    48541 tctcaatatt gcctgccgtc ccatcaacat aagcgacagt cgtacgagaa gggtcggcgc
    48601 tggaaggaac tgccgccagt tgccgcgccg tacttccctg aaccagtgag taaccattgg
    48661 ccatcgtgat gttataagtg ccgccatcct gaacgctgac ttctacacca acaatctggt
    48721 ttaattcgct caccagttga tcgcgttgat ccagcagatt gttaggtgac gcccctgccc
    48781 ccacgcctgt caggcgggag atttgatcgt tcaggctggc gatttgttta gcgtaattgt
    48841 tgatctgatc aacgctggca cctatcgcga tattgacctg tttgtcctgg tcgcgaagat
    48901 actgatcggt ggttttaaac tgattcacca acccttctga tttcccaatc agcgcctggc
    48961 gcgctgccgg gtcttccgcg ttactcacca gcgtttgcag gctggtgaag aaatcctgca
    49021 tctgtgttgc cagcgaagag gtactggtgg agagcatatt gtcgattttc gacatctgct
    49081 catagcgggc agtcaggccg ctactttgcg tctgcgccgc acgtaactgg ttggtaataa
    49141 acgcatcata ctcacgctgc acaccagaaa cgtagacacc attaccaacc cagccgccag
    49201 cgcccaacgt gctattggcc tgcgccataa tagtggtttg gcgggtatat ccggcaacgt
    49261 tatagctgga gatattatta cttgccgtat ttaacgccgc ctgggccgcg ttcagtccgc
    49321 tcatggcgtt attgatcaag ctggacatgg aggttcctta taagccttca attacagagt
    49381 attatcggca gcgacccgcc ggacttgagt tattcagaac agattatcaa tgttcatgct
    49441 gtaggttttg ctcaccttgt cgcttatcga tttcatctgc tgaatcatgt tggtgagttt
    49501 acgggcatag tgaggatcgg tggcatagcc cgcgtcctgt agggcctgcg ccccctgctc
    49561 cgcactcgcg gcggtcgtca cggcggcgta gcgcgggtta cgtgttaaca gcccaacgta
    49621 atccgacaat gcttccagat acgagctgta gacccgaaac ttcgctttta ctttcttcgc
    49681 ttcgccattt tcatattcag tcgtggtgat ttcagtgact ggccctttcc agttgccaga
    49741 ggctttgaca ccaaacaggt tatagctcgg ctcgccgttt tcgcggcgga tttgccgttg
    49801 cccccagcca gattccagcg ccgcctgagc gaggatcaaa tgatgtggca caccgctttg
    49861 ctggcttgcc agttgggcgg gcagcgagag ttgcgcgagg aatgctttac tgtcacctgg
    49921 cagcgaatca tcgtagttac gtggcacggc cttttgcacc agctgcgaaa gcgcctgatt
    49981 ttgataacgc accacggttt cgagcgggaa tttcatcggt gctgctggcg tggactcctc
    50041 tggcaatggt tgttctggcg tcatctgctt aaccatcatc tctgcaagcc ccagaccttt
    50101 gcccgtcgtc atctgttggg caatctgctg gtcatacata ctggtataca ggcgagtgtg
    50161 ctcgctgctg aacaggccat cttttggtaa cgcgtcgcgc atgcttttca acatcatctg
    50221 cacgaacatc ccttccacct gacgggccac cggacggata tttgccgccg gatcttcgct
    50281 cgctttcgcc tttagttcgt tgagtgattg cgcatcccag gccgcacttg ccagtagttt
    50341 gctgtcgctg atcatcagat gatttccagt tttgcccgca gacatcccgc actttgcatt
    50401 gattgcaaaa tagacatcag atccatcggc gtagcgccca gcgcattgag cgcgcgcacc
    50461 acgttattga ggctggcgct ggaacgtacg ctttgcagcg aaccgccgct ctggcgtaag
    50521 tcgatctgcg tttgtggcgt taccacggtc tgtccgccac caaacggtgt atctggttgg
    50581 ctgacattgg cctgacgatt gactgttact gagagatttc cctgcgctat cgcgcagctg
    50641 tcgagagtca cttcgcgatt catcaccacc gaaccggtgc gcgagttaat cactacttta
    50701 gcgtcctgcg gggtgacatt aacctgcata ttctggatat cggcaaggaa acggacctgg
    50761 gaactgttgc cactcggtac gcgcacctga atagtccgcg catccaacgc ggtggcgctg
    50821 ccatatccac gcacgcggtt gatggtgtca gcgatttgct gcgccatgct gaaatcttcg
    50881 tcgttaagtt gcaaattaag ggtattcccg acgccaaact ggctgggtaa ttcacgttca
    50941 ataactgcac cattggtgat ccgtccaccg ttcagttggt tcacctgaac actgctaccg
    51001 ccagcggagg ctcctgcgcc gccaaccaga atattgccct gcgccagcgc atacacctga
    51061 ctgtcaacgc ccttaagcgg agtcatcaac aatgtgccgc cacgcaggct tttggcattt
    51121 cccatggaag aaaccaccac gtcaatggtt tgcccctgac gtccaaacgg tggaagtgac
    51181 gccgtcacca ttaccgcagc aacgtttttt agctgcatat tggtgcccgt cggaacggta
    51241 attcccagct gtgagagcat gttattaagc gtttgtgtgg taaacggcgt ctgggttgtc
    51301 tggtcaccgg tgccatccag ccccaccacc agaccatagc caatcagtga gttttgcctt
    51361 accccctgaa cactggtgag atcgcgaata cgctcagcct gagccgccgt cgtaaccagt
    51421 agaagaatta atgcagagag aaatttaatc accacagcct cacttacatt ggcgacaggt
    51481 taaggaagaa acgctgcaac cagcccatat tttgcgcttc gttaatgtag ccattgccta
    51541 cgtattcaat gcgcgcatcc gccacctgag tagacggtac ggtattgctg ccgctgatag
    51601 tgcgtggatt aaccacgcca gagaagcgaa taaattcggt accctgatta atggcaatct
    51661 gtttttcacc caccacatgc aggttgccgt tgaccagtac ctggtcaacc gtcaccgtca
    51721 acgtgccgct aaaggtatta ctggcattgg ccccgccttt tccgttgaac gtgttaccac
    51781 cggaggcttc gacatcggca cgagcgttac caaacagccc ctgcaaatag cgcggcacag
    51841 tatcaaagcc aaaattagtt ttaccgtcac ggctggcatt cgcagaggag cttttgctgg
    51901 cgctaacgtt ctcctgcaac acgatggtca gcgtatcgcc aatattgcgt ggtcgacgat
    51961 cttcaaacag cggttgatag ccatagttaa tcggctgagc agactggaaa atagaaccgt
    52021 tggcgacggg cgtcggaccg ggaaccggtt gtgcactggt cgccccctgc accagcggcg
    52081 tggagggtat ccaggcgcag ccggttagtg aaagcaccaa caagctggaa atggcataag
    52141 tatgcgcagc gtttttttgc atggctatca tcttcaaaaa tcaataaacc ggtgaacctg
    52201 ccaccggtta tgccttagag ttgcgtcagt ttttgcagca tctgatcggt ggtggacacc
    52261 gctttactgt tgatttcgta agcgcgttgc acctgaatca tattgaccag ttcttccgcc
    52321 acgttgacgt tagacgtttc aacataccct tgatacagca gtcccgcgcc gttcaggccc
    52381 ggcgtgcttt cgttcggtgc accggaggat tgcgtttcgg tgtagaggtt ttcgccaatg
    52441 ctctccagcc cggtgtcatt cataaaggtg gtgagattga gctgcccaac ctgaaccgga
    52501 gctgcctggc cttgttgggt tacgctgacc acgccatcac gaccgatggt gatacttaac
    52561 gcattcgccg gaatggtgat cgctggctgc acctgaaaac caccagccgt caccagctgc
    52621 ccgttctgat ccacctggaa agagccgtca cgggtatagg ctgatgagcc atctggcaac
    52681 atcacctgga aaaagccctg ccctttaatc gcgacatctt tgctgttgtt ggtctgcgac
    52741 aggttgcctt ggctgtgtaa gcgttcagtg gcgaccgggc gtacccccgt gccgatttgt
    52801 aatccggagg gtaaggtggt ttgttcggaa gactgtgccc ccggctggcg aatggtttga
    52861 taaagcagat cttcaaacac cgcgcgctga cgcttaaaac cgttagtact gacgtttgcc
    52921 aggttgttgg caatgacgtc catattggtt tgctgggcgt caaggcccgt tttggcgatc
    52981 cataatgaac tgatcataaa atgtcctgta gtggattgct gacaagcaga gcagaagcac
    53041 gcggcggtgc gaatttcccc tcaccctaac cctctcccca aaggggcgag ggaacagtcc
    53101 gctcgctttt attgcaatgt catcaatcgt tcaattaact catcgacagc agttggttgg
    53161 cacggcctgc gttatcatcg acgctgctga tcaccttcat ctgcatttca aaacgccgcg
    53221 cgctggcaat catgtcgctc attgccgcaa cggcattgac gttactgcct tccagaaccc
    53281 ccgacatcac acgcaaggtt ggatctgcct gcagtaccgg tccacgcgtg gcctgggttt
    53341 ctgcgcttaa acgaaaaatg ccgtcgtcac cgcgctgcac ttcgctgccc gtggctttca
    53401 ccagtttcag acgccccact ggcgcaaccg tatttgccgg atcgcccgga ttcagcgccg
    53461 agattgttcc atcggcagct atcgtgattt ccgccccttc cggcacagca attggccccg
    53521 cctcgcctat caccggatgc ccctgaattg tcagttgccc ggtgggatca acctgaatgc
    53581 tgccattacg cgtataccct tcgctgccgt cagcggtctg cacggccaac cagccatcct
    53641 gctgcaacgc aacgtccagc gggcgcgagg tgtaatccat cttgccgggc gttatgtctg
    53701 cacccggtgt tgacgccgtt accagcgtgc gcgtgggcag tgaaagccct tccaccggca
    53761 ctgcgcgcaa ggcgtttaac tgcgcgcgaa aaccgggcgt agaggcattg gccagattac
    53821 tggcggttac cgcctgttga ttcagcgtct ggctggctgc tcccatcgcg gtataaattg
    53881 cgtgatccat tgagctatcc cgtcagcgat tagcgtaagt taaccagcgt gttgaggatc
    53941 tggtcctggg ttttgatggt ctgggcgtta gactgatagt tacgctgggc aacgatcata
    54001 ttgaccagtt ctttactgag atcgacgttg gacgcttcca acgcaccgtt ggtcagggtg
    54061 ccaaagttgc ccgtcccggc tgtccccaac agcgccacgc cagaagattg cgtcgcagac
    54121 cagacgttgt cgccttcgga tgccagacct tcgttgttgg caaagttcgc cagtacaatc
    54181 tgccccagca gttgggtttg ttcgttggaa tagttgccga caaccgtacc gtcatcattg
    54241 atttgataac tcaccagatc gcccggtttg tagccattct gggtggttgc cacaatgttg
    54301 ttagcgccgg tattttgctg catggagttg aggaagctca ggctaaacgt ggcgggttct
    54361 gcgccgttaa ttgcgccggt ggtaatattc tctgttggat ttgaggtcag aacgccattg
    54421 gcattaaaca ccagcttcat tgcaggctcg gctgtacctg ttggatcact gctatcctgg
    54481 gtgtagacct gccagttatt atccccggtc ttcacaaagt agacgctcat gtcatgagca
    54541 ttaccctgac tgtcgaaaac agtcaccgaa ccttttttgt tatagctatc cgcattgctg
    54601 gcatcaaatg cgttaacaga gggaagcgga tcgctggaat tcaggttgat ctgcatcgac
    54661 gccgtggtgg tagttttcgc tgccatcagg gtattcggga tcgaaatgtt agtcggattc
    54721 gccccttgct gaatagtcgg cggcgtaccg gttgccgggt aacccgtcag ctgtaaacct
    54781 tgcatattca ccaggttacg attttcatcc agcttaaatt gtccgttacg gctgtagaac
    54841 accgaaccgt tgctatctac cagacggaaa aaaccgttct ggctgatagc aacgtccaga
    54901 ccacgcccgg tgttggtggt cgtgccatcg gtaaagtcct gagtgatacc ggcaactttt
    54961 acccccagtc ccactttcga accggcaaac atatcggcaa aagaggccgt gcctgattta
    55021 aagccgtagg tggcggagtt ggcgatattg ttgccaataa catcgaggtt ggtggcggca
    55081 gcgtttaatc cgctaaccgc ttgagaaaag gccatgactg actcctgaaa tgtgaaggct
    55141 taaattatct gccgtacttc gtcgagggtg gtggtgccgt aagtgccgag atccagcgta
    55201 ttaccgttgt tgccgcggat cacaccctgc accagagcaa actgcagcgg ctgggcaacc
    55261 agttgtgtac caccgttact ggcgctaatc gctacgttgt aagaaccgtt cggcgcagtg
    55321 ctgccgtcag tcaacgtacc gtcccaggtg aaactgtgaa ctccggcggt cagttcacca
    55381 atatcaatgg tgcgcacaac cgcgccattt ttatcggtga tggtggccgt aactttgtct
    55441 gccgcctgtt gcagctcaac accaaacggc gtggtcgtgg tcacagcccc ttcttcactg
    55501 ccggttcccg caagaacagt ggtgccgggg atcatcacgc cgtgaccgat caggttactg
    55561 gcctgtaacg actggctgtt atcaatctgt ccggaaatag atccgagcgt ggtattgagt
    55621 ttttcaatcc cgctgaccgt gctgatttgt gccaattgcg acgtcagctc gttgttttcc
    55681 attggattgg tcgggtcctg gtttttcagc tgcgccacca acaaagtcag aaaactgctt
    55741 tgtaaatctg cggcgttgct gcccgtgagc gaactactgc tggtggtact gacgccggta
    55801 tttgtcggat cggtggtggt taccgcaatg gacatagctt tctcctttat tgaccgagcg
    55861 taagggtttt cagcatcatg cttttcaccg tgttgagcac ttcaacattg gcctgatagc
    55921 tgcgtgacgc cgacatggtg ttaaccatct ctccgacaac atcaacgttc ggcattttta
    55981 cgtagccctt tgcatccgcc agcggattac ccggttcata aaccagtttg tccggggcct
    56041 gactttctat aacatcggca acctttacgc cgcctgtcgc agcacctggt gcagcgttaa
    56101 cctggaatac cacctgtttt gcccgatatg gctgtccatc aggaccggtc acgctatcag
    56161 cattcgccag attactggcc gccacgttca ggcgctggga ctgggcagtt aacgccgacc
    56221 cggcgatatc aaaaatattc agcagtgcca tccgttaatt tccgctctgt aaaacgttca
    56281 tcatgccttt gatttgcccg ctcaacgcgc taaggctcat ctggtattgc aggctgttat
    56341 cggcaaactg ggtgcgttcg cgatccatat cgacggtatt accgtcaagc gaaggctggt
    56401 ccggaatacg gtattgcagt tctgcggtag gaggcgtcag cgcctgcgcc ggaatgtgtt
    56461 gcgttgaggt catcgtcaga gcaaccacac tggttgcatt ccgtccacgt tgcatgactt
    56521 ttttaagttc actggcaaaa tcgatatcgc gcgcctgata accaggggta tcggcattgg
    56581 cgatgtttgc tgccagcact tcctgacgct gggcgcgcag attgagcgcc tcttgttgaa
    56641 aacgtaaggc ggcgtcgagc ttatcgagca tatctcctcc gcaggtatca aaattctgcc
    56701 atcagcttaa atgcctttac gcgcgcgtta tcggcggaat aaacgcaaaa tgggtcgcta
    56761 tttatgccgt tgatggtcat tgtggacagg tacaattcac gttgtagaaa tggctggggg
    56821 cgaaaatgct ggcaataaaa cgtagcgtgg cgatcatcgc gatactgttc agtccgttaa
    56881 gtgcggcgag caatctcaca tcgcaattgc aaaccttttt tagctcccag ctcgcaggga
    56941 taagtgatga ggttcgtgtt tctattcgta cagcgcccaa tctactaccg ccgtgcgagc
    57001 agccattgct ttcgatgagc aataattccc gcttgtgggg caatgtgaat gtgttggcac
    57061 gctgcggtaa cgacaaacga tatttacagg ttaatgtgca ggccacagga aattatgtgg
    57121 ttgccgcgat gcccattgtg cgaggaggaa agctgggagc tggcaatgtc aaactgaaac
    57181 gcggacggct ggataccttg ccaccgcgta cggtgctgga tatcaatcaa cttgttgatg
    57241 ccgttagcct gcgcgatcta tcacccgatc aacctatcca gttaacccag tttcgccagg
    57301 catggcgggt aaaagcggga caacgcgtca acgtgatcgc cagcggtgat gggtttagcg
    57361 ccaacgcaga aggtcaggcg ctaaacaatg cagccgtcgc acagaatgcg cgggtgcgca
    57421 tggtatctgg acaggtagtc agcggcgttg ttgatgcaga tgggaatatt cttataaacc
    57481 tgtaagctgt taaagattac ccgtcccttg ccgataaata agcaacacat gataaaagcg
    57541 ccctcaatga ggaataaacc atgagtattg atcgcacttc gcctctgaag cccgtaagca
    57601 ccgttcaacc gcgcgaaacc actgacgcgc cggtaacgaa cacccgagcg gcaaaaacaa
    57661 cagcctccac cagcaccagt gtgacgttaa gcgacgcgca agcaaaactg atgcaacccg
    57721 gcagcagtga tatcaatctt gaacgtgtcg aagcgttaaa actggcgatt cgtaacggtg
    57781 aactaaaaat ggacaccggc aaaattgccg atgcgctgat caacgaagcg cagcaagact
    57841 tgcagagtaa ttaatcgtat gacgcgtctt gcagagatcc tcgaccagat gtccgctgtg
    57901 cttaacgatc tcaaaacggt aatggatcaa gagcagcaac atctctctat ggggcagatc
    57961 aacggcagcc agttgcaatg gattacagaa caaaaaagct cactgctggc aacgttggat
    58021 tacctcgaac agttacgcag gaaagaaccc aatacagcaa atagcgttga tattagtcaa
    58081 cgctggcagg aaattgctgt gaaaacgcag caactacgcc aaatgaatca acataacggc
    58141 tggttactgg aaggacagat tgagcgcaat caacaggcgc tggaaatgtt gaaaccgcat
    58201 caggaaccga cgctatatgg ggcgaacggt cagacctcaa caacccatcg cggcagtaaa
    58261 aagatttcga tctgaagatt atctccggcc tgcactgcag gccggaatgc attgttacac
    58321 cgtccggcgg gcaaattctt taactttgaa gcccagtacc gccagtgcag cgaagtacgc
    58381 ggcaatcccc gccagcacga ccgccattaa acgcagcaaa cgccagggca tggtacccaa
    58441 tgaccactcc ggcatgatat gtaacatacc taaaagcacg ccagacatca ccagtaccgc
    58501 caccaccaga cgcaacagaa acgccatcca gccaggttgc ggggtaaaga ttttctgctt
    58561 acgcaactgc cagtaaagca gcgaagcatt cagacacgcc gccagaccaa tagaaagtga
    58621 cagcccggca tgtttcaacg gaccgataaa cgccaggttc atcaattgcg ttaaaatcag
    58681 cgtaacgatg gcaattttca ctggcgtttt aatgtcctgg cgggaataaa agccaggagc
    58741 caacactttc actacaatca ggccaatcaa acccaccgag taggcaatta acgcccgctg
    58801 ggtcatcagc gcatcaaagg cggtaaattt accgtactgg aacagcgaaa cggttaacgg
    58861 accggaaaga atgcccaacg caaccgcact cggcaacgcc aacaggaaac aaagacgcag
    58921 tccccagtcc atcaaacggt tgtattcatc atgattgcca ctggcaaaac ttttcgacag
    58981 cgacggtagc aaaatggtgc caagcgccac gcccagcaca ccggacggaa actccattaa
    59041 gcggtcggcg taatacatcc atgacaccga accggaagca agaaacgagg caaaaatggt
    59101 gttgatgatt aaggagatct ggctcacaga gacgccaagg attgccggtc ccatctgttt
    59161 caccacacgc attgctccgg catcgtggaa gttaatgcgt ggcaaaacca gcatgccgat
    59221 cttcttcaga tgcggtagct gatacaccag ctgcaggacg ccgccgaccg tcacagccca
    59281 cgccagcgcc agcaccggtg ggttaaagta cggtgcggca aacagcgcga aaccaatcat
    59341 gctgatgtta agcagtgttg gagcaaacgc cggaatcgag aagcggttcc acgtattcag
    59401 aatcgctccc accagcgacg ccagggagat cagcaagata tagggaaagg taatctttag
    59461 tagctggctg gtcagggcaa atttgtcagc tgtgtcagcg aagcctggcg cggtcaccat
    59521 gatcacccac ggtgcggcga gcatgccagc gaccgtcaca accgccagcg caagtgtcag
    59581 caggccagaa acataagaga caaagacccg cgtggcgtct tcaccctgct tacttttata
    59641 ttccgccaga atcggtacaa atgcctggga aaatgcccct tcggcaaaga tacggcgtaa
    59701 caagttagga agtttaaaag cgacgaaaaa ggcgtcggtt gccatccctg cgccaaagat
    59761 tctggcgaca attgcgtctc gtgcgaagcc aagcacacgc gaaaacatgg tcatcgagct
    59821 gacggcggcc agcgatttta ataaattcat cggtgttcta atccagaccc acgggcaaaa
    59881 cgcctgcaag gcaggcgctg aaagaggcga atagtctacc tggattatgg tgaattgcta
    59941 ccgccagatg ttacagggtt tattcactca tcgcatcgcg ccagatcttg tcaacgatac
    60001 gttgcgccag cactgcctgt tcgccggcgg tttgcggaac tgtctgattt tgcacgcatt
    60061 caatgaagtg ccgcgcacag ccgacaaatc cacgttgctc aagcgtgctc tgccaaccag
    60121 gaatcggttt attcactaca ccctgcccac gctcctcacg ccattcgcgc atttccgtaa
    60181 tgtcgatgag cgcaccgtca gtcacggcct gcacggtttc acgctgactt ccggcacggc
    60241 gatgcataca agtggtgatc tgcaaaggac cggccgaaaa atggtgctcg gcaaacagca
    60301 tttcgccagc gtcgttagtc agtagcgtac cgccatccag agaggctttg ccgcccgaca
    60361 accacagcgc ggtatccacc acatgcagat aatcatccag caacgtgaaa taaagatcgt
    60421 gtggcccgac gctattgcta cgatgtttat ccattcttag cgaggctgcg gtggcgagtt
    60481 gcgtttttaa ctcacagtag agtggtgcga aacgacggtt aaaaccgacc atcaacgtca
    60541 gttttttccg cgccgccagt tccaccagcc gttcagcatc gcgcagattt tctgccagcg
    60601 gtttatcgac acagacatgt acccccgcat tgagtaacgt actgaccacg tcaaagtgac
    60661 tggcggtgct ggaatgtaca aaaaccgcat cgcaactggc ggcaaggctg gataacgaat
    60721 cggcataagg aatgcgccag ctttcacaaa ttggcagggc tttcgcgcgc gtaggcgacc
    60781 aggctccttg taacgtccag tcagacgctg ccgccagcac cggtaaccac gctttttgcg
    60841 caatgccgcc taatcccact acgccgatac gtaatttttt cactttaatc tcccaggtgg
    60901 gccagcaacg aatcaagacg ctgtttcagt tctgccactt cgatttccag ggcttcgacg
    60961 cgggcctgta aatcaccgtc aaccgcgttc gacatatccg ttaccgccgg ctgatcctct
    61021 acctcaccac tgaaaagatg catgtagcgg ctttcgcgtt tacccggttc gcgggccaga
    61081 cgcaccacaa aaggaccatc ttcgcgattc gccagttgtt ccagcgtcaa ctccacttcc
    61141 gccatatcgc tgaattcata cattcgcgcg gcgcggctgc gcagttcacc cggcgtctgg
    61201 gcaccacgca ataacaacgt ggtgattaac gccacttctg cggcgctcag tttcagatcg
    61261 ccaaattctg aattacaaaa acgttgctca tatttggtga cccgattacc aaaaccgctc
    61321 actgtgcgta gataatgacg tttgaccaga ttatccagct gttcctgcac ttcagattca
    61381 ctcagattca tgaccggttc acggttcgtt ttctgattac aggccgtgac tacaccattg
    61441 actgagagcg ggtattgctc cggcgtggtc acctgctttt ccagcaagca accaatcaca
    61501 cgagcttcca gggcagtaag ttgatatttc atgagattct ccttagcggc cgggcgtcca
    61561 gtctggggta gttaatgccg tcagtacgtg atcgcgccat tgtccatcaa tcaacagata
    61621 gtctttcgca tagccttctt tttcaaaacc cagtcgcgcc agtaaatcac cgctgcgttt
    61681 attgtgcggc atataattag ccataatgcg atgaatatgt tgggtgcgct gcatataacg
    61741 aatggctgcg gtcagggctt caaacatgag tcctttgccc tgccattttt gcccaatcga
    61801 ataaccgaga tagcaggcat gaaaagagcc gcgaacaaca ttggaaaaat tggcaacgcc
    61861 aataatctct ttttcgtccg ggtcgaataa gccaaagtag aaagctgaac cttgtttatg
    61921 aaattcgtta atcatcccca gcctggcctg ccagcctgat ggataacagt ggctttcgtc
    61981 gcgcactggc tcccagggct tgaggaaatg gcgattctct gcgtaataat ccgccagacg
    62041 ccaggcatca cgatcatgca ccagacgcac gaccagtcgg tctgtggtta agcgcacttt
    62101 tggcacgtta ctgcgatagc caaacattct atacctactc cttcccgtaa cgtctttaat
    62161 acgctgtaag tattactata cctgcgcaac cagaccctgt gaaaagtcca aaatgccatt
    62221 ttctgcatta ttcacatatt tgataaaaac tctctatcaa agcgacgttt tgataaaaaa
    62281 atattgtccc cgaagtgatg ggaaaagtct tagcggcaat gcagaataga aagtgctcat
    62341 cttttatttc ccgggagggg aaatgtcccg cgtgtcgcag gcgaggaacc tgggtaaata
    62401 tttcctgctc atcgataata tgctggtcgt gctggggttc tttgttgtct tcccgctgat
    62461 ctctatccgc ttcgttgatc aaatgggctg ggccgccgtc atggtcggta ttgccctcgg
    62521 cctacgccag tttattcagc aaggtctggg tattttcggc ggcgcaattg ccgaccgctt
    62581 tggtgccaaa ccgatgattg ttaccggtat gctgatgcgt gccgccggat tcgccacaat
    62641 gggtatcgcc cacgaaccgt ggctattgtg gttttcatgc ctgctctcgg gactcggtgg
    62701 cacgttgttt gatccgccgc gttcggcgct ggtggtgaaa ctaatccgtc cacaacagcg
    62761 tggtcgtttt ttctcgctgt tgatgatgca ggacagtgcc ggtgcggtca ttggcgcatt
    62821 gttggggagc tggctgttgc aatacgactt tcgcctggtc tgcgccacag gggccgtttt
    62881 gtttgtgctg tgtgcagcgt tcaatgcgtg gttgttaccg gcatggaaac tctccaccgt
    62941 acgcacgccc gttcgcgaag gcatgacccg cgtgatgcgc gacaagcgtt ttgtcaccta
    63001 tgtcctgacg ctggcgggtt actacatgct ggctgtacaa gtgatgctga tgctgccaat
    63061 tatggtcaac gatgtggctg gcgcgccctc tgccgttaaa tggatgtatg ccattgaagc
    63121 gtgtctgtcg ttaacgttgc tctaccctat cgcccgctgg agtgaaaagc attttcgtct
    63181 ggaacaccgg ttgatggctg ggctgttgat aatgtcatta agcatgatgc cggtgggcat
    63241 ggtcagcggc ctgcaacaac ttttcaccct gatttgtctg ttttatatcg ggtcgatcat
    63301 tgccgagcct gcgcgtgaaa ccttaagtgc ttcgctggcg gacgcaagag ctcgcggcag
    63361 ctatatgggg tttagccgtc tgggtctggc gattggcggc gctattggtt atatcggtgg
    63421 cggctggctg tttgacctgg gcaaatcggt gcaccagcca gagcttccgt ggatgatgct
    63481 gggcattatt ggcatcttca ctttccttgc gctgggttgg cagtttagcc agaaacgcgc
    63541 cgcgcgtcgt ttgcttgaac gcgacgcctg atttgatact cccccctgac tcctccatac
    63601 tgaaagtgaa ctgacagcca aatggaggag tcacgtgaag ctatacattt acgatcactg
    63661 cccttactgc ctcaaagccc gcatgatttt cggcctgaaa aatatccccg tcgaattaca
    63721 tgttctgctc aacgacgacg cagaaacacc cacccggatg gtcggtcaaa aacaggttcc
    63781 cattctgcaa aaagatgaca gccgctatat gccagaaagc atggatatcg ttcactatgt
    63841 cgataaactc gacggcaaac cgttactgac cggcaaacgt tcccctgcca ttgaagagtg
    63901 gctgcgcaag gtcaatggct acgccaacaa actgctgttg ccgcgttttg ccaaatcggc
    63961 atttgatgag ttttctactc ccgccgcgcg caaatatttc gtcgacaaga aagaggccag
    64021 cgcgggtaat tttgccgacc tgctggccca ctctgacggt ctgattaaga atatcagcga
    64081 tgatttacgt gcgctggaca aactgatcgt caaaccgaac gccgtgaatg gcgaactttc
    64141 ggaagatgat attcagctat tcccgctact gcgtaatctg acgctggtag ccggaattaa
    64201 ctggccaagc cgcgttgctg attaccgcga taatatggcg aaacagacac aaatcaattt
    64261 gttatcatca atggcgattt aatcctgact cgccgcggca gcctgtattc gcgggttgcc
    64321 gccgttttca gttttttctg ctggcgagtt tatcgctggt gacacatgct gcgttgtgtc
    64381 aggatattga cgcagatttc atgtcgagga ctaccatgaa caagttttta tttgccgctg
    64441 cattgatcgt cagtggcctg ctcgttggct gtaatcaact cacccaatac accatcaccg
    64501 aacaagaaat taaccagtcg cttgcgaaac ataataattt ctcaaaagat attggtttac
    64561 ccggtgtggc tgacgcccat attgttctga caaacctgac cagccaaatt ggtcgcgaag
    64621 agccgaataa ggttgcccta accggagacg ccaatctgga catgaactcc ctgttcggta
    64681 gccagaaagc gaccatgaaa ctgaagctga aagcgctgcc cgtgtttgat aaagaaaaag
    64741 gcgcgatctt cctgaaagag atggaggtgg tcgatgcgac ggtacaaccg gaaaaaatgc
    64801 aaacggtgat gcaaacgttg cttccctatc tgaaccaggc attacgcaat tactttaacc
    64861 agcaacctgc ttacgtcctg cgcgaagatg gcagccaggg cgaagcaatg gcgaaaaaac
    64921 tggcgaaagg cattgaagtg aagccaggcg aaattgtcat tccatttact gattaatcac
    64981 gagggcgcat tcgcgccctt tatttttcgt gcaaaggaaa acgtttccgc ttatcctttg
    65041 tgtccggcaa aaacatccct tcagccggag catagagatt aatgactgca ccatcccagg
    65101 tattaaagat ccgccgccca gacgactggc accttcacct ccgcgatggc gacatgttaa
    65161 aaactgtcgt gccgtatacc agcgaaattt atggacgggc tatcgtaatg cccaatctgg
    65221 ctccgcccgt gaccaccgtt gaggctgccg tggcgtatcg ccaacgtatt cttcacgccg
    65281 tacctgccgg gcacgatttc accccattga tgacctgtta tttaacagat tcgctggatc
    65341 ctaatgagct ggagcgcgga tttaacgaag gcgtgttcac cgctgcaaaa ctttacccgg
    65401 caaacgcaac cactaactcc agccacggcg tgacgtcagt tgacgcaatc atgccggtat
    65461 tagagcgcat ggaaaaaatc ggtatgccgc tactggtgca tggtgaagtg acacatgcag
    65521 atatcgacat ttttgatcgt gaagcgcgct ttatagaaag cgtgatggaa cctctgcgcc
    65581 agcgcctgac cgcgctgaaa gtcgtttttg agcacatcac caccaaagac gcagccgact
    65641 atgtccgtga cggaaatgaa cggctggctg ctaccatcac tccgcaacat ctgatgttta
    65701 accgcaacca tatgctggtt ggtggcgtgc gtccgcacct gtattgtcta cccatcctca
    65761 aacgcaatat tcaccaacag gcattgcgtg aactagtcgc cagcggtttt aatcgagtat
    65821 tcctcggtac ggattctgcg ccacatgcac gtcatcgcaa agagagcagc tgcggctgcg
    65881 cgggctgctt caacgcccca accgcgctgg gaagttacgc taccgtcttt gaagagatga
    65941 atgctctgca gcactttgaa gcattctgtt ctgtaaacgg cccgcagttc tatgggttac
    66001 cggtcaacga cacattcatc gaactggtac gtgaagagca tcaggttgct gaaagcatcg
    66061 cactgactga tgacacgctg gtgccattcc tcgccgggga aacggtacgc tggtctgtta
    66121 aacaataaaa atcg
//
LOCUS       DBFIFI010000005        66043 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.005, whole genome shotgun sequence.
ACCESSION   DBFIFI010000005 DBFIFI010000000
VERSION     DBFIFI010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 66043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66043
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.005"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..428
                     /gene="cmtB"
                     /locus_tag="N7B39_000300"
     CDS             <1..428
                     /gene="cmtB"
                     /locus_tag="N7B39_000300"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HGU2420356.1"
                     /translation="FPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQAIKDST
                     INNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIGLSAADA
                     DSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            456..1844
                     /gene="cmtA"
                     /locus_tag="N7B39_000301"
     CDS             456..1844
                     /gene="cmtA"
                     /locus_tag="N7B39_000301"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HGU2420357.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            1859..3136
                     /gene="yggP"
                     /locus_tag="N7B39_000302"
     CDS             1859..3136
                     /gene="yggP"
                     /locus_tag="N7B39_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HGU2420358.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVELAASKGIELVYVNTKGMSDPVQMLRALTGDAGFDDIFVYAAVSAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPETVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            3133..4098
                     /gene="glpX"
                     /locus_tag="N7B39_000303"
     CDS             3133..4098
                     /gene="glpX"
                     /locus_tag="N7B39_000303"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HGU2420359.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRDVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGNMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDVLVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLY"
     gene            4120..4629
                     /gene="fumE"
                     /locus_tag="N7B39_000304"
     CDS             4120..4629
                     /gene="fumE"
                     /locus_tag="N7B39_000304"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HGU2420360.1"
                     /translation="MATLSEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEESVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLKEQD
                     ETLGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            4626..5339
                     /locus_tag="N7B39_000305"
     CDS             4626..5339
                     /locus_tag="N7B39_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HGU2420361.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWAYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPIIIVEGNWLLLDDDN
                     WRQLAQFCDFSIFINAPATALRERLVGRKLAGGLSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(5311..5988)
                     /locus_tag="N7B39_000306"
     CDS             complement(5311..5988)
                     /locus_tag="N7B39_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU2420362.1"
                     /translation="MLTLNQISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKIGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTASAHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGLIRNIN
                     PPDDIHP"
     gene            complement(5982..6659)
                     /locus_tag="N7B39_000307"
     CDS             complement(5982..6659)
                     /locus_tag="N7B39_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU2420363.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(6647..7354)
                     /locus_tag="N7B39_000308"
     CDS             complement(6647..7354)
                     /locus_tag="N7B39_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HGU2420364.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWTHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALTIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            complement(7355..7933)
                     /locus_tag="N7B39_000309"
     CDS             complement(7355..7933)
                     /locus_tag="N7B39_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HGU2420365.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKTLTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(7956..8387)
                     /locus_tag="N7B39_000310"
     CDS             complement(7956..8387)
                     /locus_tag="N7B39_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HGU2420366.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQQQLHAAWIA
                     GCTGSLTDIALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            8759..9778
                     /gene="epd"
                     /locus_tag="N7B39_000311"
     CDS             8759..9778
                     /gene="epd"
                     /locus_tag="N7B39_000311"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HGU2420367.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            9828..10991
                     /gene="pgk"
                     /locus_tag="N7B39_000312"
     CDS             9828..10991
                     /gene="pgk"
                     /locus_tag="N7B39_000312"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HGU2420368.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDEILSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            11206..12285
                     /gene="fbaA"
                     /locus_tag="N7B39_000313"
     CDS             11206..12285
                     /gene="fbaA"
                     /locus_tag="N7B39_000313"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HGU2420369.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(12349..12447)
                     /locus_tag="N7B39_000314"
                     /pseudo
     CDS             complement(12349..12447)
                     /locus_tag="N7B39_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12643..13503
                     /gene="mscS"
                     /locus_tag="N7B39_000315"
     CDS             12643..13503
                     /gene="mscS"
                     /locus_tag="N7B39_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HGU2420370.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            13642..14277
                     /gene="argO"
                     /locus_tag="N7B39_000316"
     CDS             13642..14277
                     /gene="argO"
                     /locus_tag="N7B39_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HGU2420371.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            14370..15110
                     /gene="yggE"
                     /locus_tag="N7B39_000317"
     CDS             14370..15110
                     /gene="yggE"
                     /locus_tag="N7B39_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HGU2420372.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            15277..16173
                     /gene="ygfI"
                     /locus_tag="N7B39_000318"
                     /pseudo
     CDS             15277..16173
                     /gene="ygfI"
                     /locus_tag="N7B39_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(16170..17648)
                     /gene="scpC"
                     /locus_tag="N7B39_000319"
     CDS             complement(16170..17648)
                     /gene="scpC"
                     /locus_tag="N7B39_000319"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HGU2420373.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHNEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(17672..18457)
                     /gene="scpB"
                     /locus_tag="N7B39_000320"
     CDS             complement(17672..18457)
                     /gene="scpB"
                     /locus_tag="N7B39_000320"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HGU2420374.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(18468..19463)
                     /gene="meaB"
                     /locus_tag="N7B39_000321"
     CDS             complement(18468..19463)
                     /gene="meaB"
                     /locus_tag="N7B39_000321"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HGU2420375.1"
                     /translation="MINEATLAESIRRLRQGEHATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNTLRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(19456..21600)
                     /gene="scpA"
                     /locus_tag="N7B39_000322"
     CDS             complement(19456..21600)
                     /gene="scpA"
                     /locus_tag="N7B39_000322"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HGU2420376.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKTGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIIAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEAAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGLDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(21804..22697)
                     /gene="argP"
                     /locus_tag="N7B39_000323"
     CDS             complement(21804..22697)
                     /gene="argP"
                     /locus_tag="N7B39_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HGU2420377.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            23080..23739
                     /gene="rpiA"
                     /locus_tag="N7B39_000324"
     CDS             23080..23739
                     /gene="rpiA"
                     /locus_tag="N7B39_000324"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HGU2420378.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            23994..25226
                     /gene="serA"
                     /locus_tag="N7B39_000325"
     CDS             23994..25226
                     /gene="serA"
                     /locus_tag="N7B39_000325"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HGU2420379.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            25455..25514
                     /gene="ibsC"
                     /locus_tag="N7B39_000326"
     CDS             25455..25514
                     /gene="ibsC"
                     /locus_tag="N7B39_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HGU2420380.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            25848..25907
                     /locus_tag="N7B39_000327"
     CDS             25848..25907
                     /locus_tag="N7B39_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HGU2420381.1"
                     /translation="MMRLVIILIVLLLISLPAY"
     gene            complement(26008..26610)
                     /gene="fau"
                     /locus_tag="N7B39_000328"
     CDS             complement(26008..26610)
                     /gene="fau"
                     /locus_tag="N7B39_000328"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HGU2420382.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(26631..26814)
                     /gene="ssrS"
                     /locus_tag="N7B39_000329"
     ncRNA           complement(26631..26814)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="N7B39_000329"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(26856..27185)
                     /gene="zapA"
                     /locus_tag="N7B39_000330"
     CDS             complement(26856..27185)
                     /gene="zapA"
                     /locus_tag="N7B39_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HGU2420383.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            27353..27931
                     /gene="ygfB"
                     /locus_tag="N7B39_000331"
     CDS             27353..27931
                     /gene="ygfB"
                     /locus_tag="N7B39_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HGU2420384.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            27957..29282
                     /gene="pepP"
                     /locus_tag="N7B39_000332"
     CDS             27957..29282
                     /gene="pepP"
                     /locus_tag="N7B39_000332"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HGU2420385.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECEMRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DIDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            29279..30457
                     /gene="ubiH"
                     /locus_tag="N7B39_000333"
                     /gene_synonym="visB"
     CDS             29279..30457
                     /gene="ubiH"
                     /locus_tag="N7B39_000333"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HGU2420386.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLAHCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLEGGETLTGRVLVA
                     ADGTHSALATVCGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTHAARS
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERGEDMGDYGVLCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWTPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            30481..31683
                     /gene="ubiI"
                     /locus_tag="N7B39_000334"
                     /gene_synonym="visC"
     CDS             30481..31683
                     /gene="ubiI"
                     /locus_tag="N7B39_000334"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HGU2420387.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPVKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(31757..31840)
                     /locus_tag="N7B39_000335"
     CDS             complement(31757..31840)
                     /locus_tag="N7B39_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2420388.1"
                     /translation="MSKNTKSKIRDIRKYNAKTEVKLVYFK"
     gene            32131..33225
                     /gene="gcvT"
                     /locus_tag="N7B39_000336"
     CDS             32131..33225
                     /gene="gcvT"
                     /locus_tag="N7B39_000336"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HGU2420389.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHEVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEGGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPKGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            33249..33638
                     /gene="gcvH"
                     /locus_tag="N7B39_000337"
     CDS             33249..33638
                     /gene="gcvH"
                     /locus_tag="N7B39_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HGU2420390.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            33756..36629
                     /gene="gcvP"
                     /locus_tag="N7B39_000338"
     CDS             33756..36629
                     /gene="gcvP"
                     /locus_tag="N7B39_000338"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HGU2420391.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDIFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPIGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(36795..38228)
                     /gene="bglA"
                     /locus_tag="N7B39_000339"
     CDS             complement(36795..38228)
                     /gene="bglA"
                     /locus_tag="N7B39_000339"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HGU2420392.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINLEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDSDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQKPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGENL"
     gene            38273..38584
                     /gene="yqfB"
                     /locus_tag="N7B39_000340"
     CDS             38273..38584
                     /gene="yqfB"
                     /locus_tag="N7B39_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HGU2420393.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELKKVIADIYPGQTQFYVIEFKC
                     L"
     gene            38748..39407
                     /gene="yqfA"
                     /locus_tag="N7B39_000341"
     CDS             38748..39407
                     /gene="yqfA"
                     /locus_tag="N7B39_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HGU2420394.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(39484..40464)
                     /gene="ygfZ"
                     /locus_tag="N7B39_000342"
     CDS             complement(39484..40464)
                     /gene="ygfZ"
                     /locus_tag="N7B39_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HGU2420395.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYLQGQ
                     VTADVSQMTDDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLAELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANIFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLTGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLCIEPLPYSLEE"
     gene            40707..40973
                     /gene="sdhE"
                     /locus_tag="N7B39_000343"
     CDS             40707..40973
                     /gene="sdhE"
                     /locus_tag="N7B39_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HGU2420396.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            40954..41361
                     /gene="ygfX"
                     /locus_tag="N7B39_000344"
     CDS             40954..41361
                     /gene="ygfX"
                     /locus_tag="N7B39_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HGU2420397.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     SGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(41401..41922)
                     /gene="fldB"
                     /locus_tag="N7B39_000345"
     CDS             complement(41401..41922)
                     /gene="fldB"
                     /locus_tag="N7B39_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HGU2420398.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            42034..42930
                     /gene="xerD"
                     /locus_tag="N7B39_000346"
     CDS             42034..42930
                     /gene="xerD"
                     /locus_tag="N7B39_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HGU2420399.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            42955..43665
                     /gene="dsbC"
                     /locus_tag="N7B39_000347"
     CDS             42955..43665
                     /gene="dsbC"
                     /locus_tag="N7B39_000347"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HGU2420400.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            43671..45404
                     /gene="recJ"
                     /locus_tag="N7B39_000348"
     CDS             43671..45404
                     /gene="recJ"
                     /locus_tag="N7B39_000348"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HGU2420401.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQIEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            45495..46593
                     /gene="prfB"
                     /locus_tag="N7B39_000349"
     CDS             join(45495..45569,45571..46593)
                     /gene="prfB"
                     /locus_tag="N7B39_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HGU2420402.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            46603..48120
                     /gene="lysS"
                     /locus_tag="N7B39_000350"
     CDS             46603..48120
                     /gene="lysS"
                     /locus_tag="N7B39_000350"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HGU2420403.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIVESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(48163..48711)
                     /gene="idi"
                     /locus_tag="N7B39_000351"
     CDS             complement(48163..48711)
                     /gene="idi"
                     /locus_tag="N7B39_000351"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HGU2420404.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            48766..48837
                     /locus_tag="N7B39_000352"
     CDS             48766..48837
                     /locus_tag="N7B39_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2420405.1"
                     /translation="MINQASVYRQPPVLSGCRQVKTI"
     gene            complement(48834..48959)
                     /gene="yqfG"
                     /locus_tag="N7B39_000353"
     CDS             complement(48834..48959)
                     /gene="yqfG"
                     /locus_tag="N7B39_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HGU2420406.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(48961..50409)
                     /gene="uacT"
                     /locus_tag="N7B39_000354"
     CDS             complement(48961..50409)
                     /gene="uacT"
                     /locus_tag="N7B39_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HGU2420407.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAQLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            50845..52764
                     /gene="ygfT"
                     /locus_tag="N7B39_000355"
     CDS             50845..52764
                     /gene="ygfT"
                     /locus_tag="N7B39_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HGU2420408.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGLAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQQIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKNHFGEIYCGLDPQQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            52764..53252
                     /gene="ygfS"
                     /locus_tag="N7B39_000356"
                     /pseudo
     CDS             52764..53252
                     /gene="ygfS"
                     /locus_tag="N7B39_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
     gene            complement(53288..54655)
                     /gene="ghxQ"
                     /locus_tag="N7B39_000357"
                     /gene_synonym="ygfQ"
     CDS             complement(53288..54655)
                     /gene="ghxQ"
                     /locus_tag="N7B39_000357"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HGU2420409.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(54691..56007)
                     /gene="guaD"
                     /locus_tag="N7B39_000358"
     CDS             complement(54691..56007)
                     /gene="guaD"
                     /locus_tag="N7B39_000358"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HGU2420410.1"
                     /translation="MSGEHTLKAVRGSFIDVARTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLKEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(56025..57425)
                     /gene="xanQ"
                     /locus_tag="N7B39_000359"
     CDS             complement(56025..57425)
                     /gene="xanQ"
                     /locus_tag="N7B39_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HGU2420411.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(57590..60460)
                     /gene="xdhD"
                     /locus_tag="N7B39_000360"
     CDS             complement(57590..60460)
                     /gene="xdhD"
                     /locus_tag="N7B39_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HGU2420412.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIEVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVDMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEPVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(60457..61236)
                     /gene="ygfM"
                     /locus_tag="N7B39_000361"
     CDS             complement(60457..61236)
                     /gene="ygfM"
                     /locus_tag="N7B39_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HGU2420413.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(61286..62614)
                     /gene="ssnA"
                     /locus_tag="N7B39_000362"
     CDS             complement(61286..62614)
                     /gene="ssnA"
                     /locus_tag="N7B39_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HGU2420414.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPSPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVN
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(62617..65715)
                     /gene="ygfK"
                     /locus_tag="N7B39_000363"
     CDS             complement(62617..65715)
                     /gene="ygfK"
                     /locus_tag="N7B39_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HGU2420415.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVHGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVAAHGVKFEYGCSPD
                     LTVEQLKNQDFHYVLIATGTDKNSGVKLAGDNQNVLKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATVVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLAQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
BASE COUNT        16001 a        16559 c        17561 g        15922 t
ORIGIN      
        1 attttccaga atcatcaatc tcagttatac attcagcaaa agattggcag gaagctatcg
       61 atttctcgat ggtatcattg ctggataaaa actatatcag cgagaattac attcaggcaa
      121 ttaaagattc caccattaac aatggccctt attatattct cgcaccaggc gtggcaatgc
      181 ctcatgcgcg accggaatgt ggggcgctta aaaccgggat gtctttgaca ttacttgaac
      241 aaggtgttta ttttccgggg aatgacgaac caattaaatt actcatcgga ctctctgctg
      301 ccgatgccga ttcgcacatt ggcgctattc aggcgttaag tgaattactg tgcgaagaag
      361 aaatactcga acaactctta acagcatcat cagaaaaaca attagcggac attatcagcc
      421 gcggataatt actctctcct ctacaggata atattatgga aaacaagtct gctcgtgcaa
      481 aggtccaggc ttttgggggc tttttgactg caatggtcat ccccaatatt ggtgctttta
      541 ttgcctgggg ttttattact gcgttattta ttcccaccgg ttggctgcct aacgaacatt
      601 tcgccaaaat tgtcggcccg atgattacct atttattgcc cgtgatgatt ggttctaccg
      661 gtggtcatct ggtcggcggt aaacgcggcg cggtcatggg cggaataggt actattggtg
      721 tgatcgtcgg cgcagagatc ccgatgttcc ttggctcaat gattatgggg ccgctcggtg
      781 gtctggtcat aaaatatgtt gataaggcac tggaaaaacg catacctgcc ggttttgaga
      841 tggttatcaa taacttctca ttaggtatcg cggggatgct cctttgcctg ctgggttttg
      901 aagtgatcgg cccggcggtg ttaattgcca atactttcgt caaagagtgt attgaggcgc
      961 tggtacatgc gggttatctg cctctgctgt cagtcatcaa tgaaccggcg aaagtgcttt
     1021 tccttaataa tgcgatcgat cagggcgtct attatccgct gggaatgcaa caggcttcgg
     1081 ttaacggtaa atccatcttc tttatggtgg cctctaaccc aggtccgggc ctggggctgc
     1141 tgctggcgtt taccttgttt ggtaaaggga tgagtaaacg ttctgcgccc ggggcgatga
     1201 ttattcactt cctcggtggg atccacgaac tgtatttccc atatgtgctg atgaagccgc
     1261 tgactattat tgccatgatt gcgggcggta tgtctggcac ctggatgttt aacttactgg
     1321 acggtggtct ggtggctggc ccaagtccgg ggtctatctt tgcttacctg gcgctgacgc
     1381 cgaaaggttc gttcctggcg acaattgccg gtgttacggt aggtaccctg gtgtcctttg
     1441 ctatcacttc gctgatactg aagatggaaa aaacggtgga aacggagagc gaagatgagt
     1501 ttgctcagtc agccaatgcg gttaaggcga tgaaacaaga gggtgcattc tcgttaagca
     1561 gggttaagcg tatcgccttt gtttgcgatg cggggatggg ctccagtgcg atgggcgcga
     1621 ccaccttccg taaacgcctg gaaaaagcgg ggctggcaat tgaagtaaaa cattacgcca
     1681 tagaaaacgt gcctgcggat gcggatatcg tcgttactca tgccagtctg gaagggcgcg
     1741 tgaaacgtgt gacggataaa ccactgatat tgattaataa ctatattggc gatccaaaac
     1801 tcgacacttt atttaatcaa ttaaccgccg aacataaaca ctgattacag aggtaaaaat
     1861 gaaaaccaaa gttgctgcta tttatggcaa gcgggatgtc cgtctgcgcg aatttgaact
     1921 gccagaaatt accgataatg aattactggt gagtgtgatt tctgacagcg tctgtttatc
     1981 cacctggaaa gcggcgttac tcggtagtga acataaacgc gtacccgacg atttagaaaa
     2041 tcatccggtc attaccgggc atgaatgtgc cggggttatt gtcgaagtgg gtaaaaatct
     2101 cactggcaaa tataaaaaag gccagcgttt tgtattgcaa ccggcgatgg ggttaccaag
     2161 cggatattca gcgggctaca gctacgaata ttttggcggc aatgccactt atatgattat
     2221 tcccgaaatc gccattaatt tgggctgcgt attaccgtat cacggctctt attttgctgc
     2281 ggcgtcgctg gcagagccta tgtgctgcat tattggcgct tatcatgcca attatcacac
     2341 cacgcaatat gtttatgagc atcgcatggg cgtcaaacct ggcggcaata ttgcactgct
     2401 ggcgtgtgca ggtccgatgg gcattggcgc tatcgattac gccattaacg gcggcataca
     2461 accgtcgcgg gtggtggtgg tcgatatcga cgacaaacgt ctggcgcagg tacagaagct
     2521 gctgccggtg gaactggcgg ccagtaaagg cattgagctg gtgtatgtga ataccaaagg
     2581 gatgagcgat cctgtccaga tgctgcgggc gctgacagga gatgccgggt tcgatgacat
     2641 ttttgtttat gcggcggtgt ctgctgtcgt tgagatggct gatgaattac tggcggaaga
     2701 tggctgtctg aacttctttg ccgggccgac ggataaaaac ttcaaagtgc cgtttaattt
     2761 ctacaacgtt cattacaaca gcacgcacgt cgtcggtaca tccggcggtt caacggacga
     2821 catgaaagag gcgattgccc ttagcgccac agggcagtta cagccctcgt ttatggtgac
     2881 ccatatcggt ggcctggatg cggtgccaga aaccgtgctc aatctgccgg atatccctgg
     2941 cggtaaaaaa ctcatttata acggcgtgac catgccgctc actgccattg ccgattttgc
     3001 cgaaaaaggc aaaaccgatc cgctgtttaa agagttggcg cggctggttg aggaaacgca
     3061 cggcatctgg aatgaacagg ccgagaaata tctgctggca caatttggcg ttgatatcgg
     3121 ggaggccgcg caatgatgtc cctggcgtgg ccattatttc gcgttacgga acaggcagcg
     3181 ttggctgcct ggccgcaaac cggatgtggc gacaaaaata aaattgatgg cctggcggtc
     3241 accgcgatgc gccaggcatt aaacgacgtc gcttttcgtg gacgagtggt tatcggcgaa
     3301 ggagaaattg accatgcgcc gatgctgtgg attggcgaag aggtgggcaa aggcgatggg
     3361 ccagaagtcg atatcgcggt tgatcccatt gaaggcacgc ggatggtggc gatggggcag
     3421 agcaatgcgc tggcggtcat ggctttcgcc cctcgagata gcctgttaca tgcgcccgat
     3481 atgtatatga aaaagctggt tgttaatcga ctggctgccg gggcgattga tctgtctttg
     3541 cctctggcgg acaacttgcg tgacgtggcg cgggcgttag gtaaaccgct ggacaagctg
     3601 cgcatggtta cgctggataa accgcgtctg agtgccgcga ttgaagaagc aacacagctg
     3661 ggcgtgaagg tttttgccct gccggatggc gatgtcgccg ccagcgtgct gacctgctgg
     3721 caggataatc cttacgatgt gatgtacacc atcggcggtg cgccggaagg cgtgatttct
     3781 gcctgtgccg ttaaagcatt aggcgggaat atgcaggccg agctgattga cttttgtcag
     3841 gccaaagggg attacacgga aaatcggcag attgcagagc aggagcgcaa gcgttgcaag
     3901 gcaatgggtg ttgacgttaa ccgtgtgtac tcgctcgatg tactggtaag ggggaacgat
     3961 atcctcttta gcgctacggg cgtcacgggc ggcgagttgg tgaacggtat ccaacagacg
     4021 gcgaatggag tgcggacgca gacattactg atcggcggcg cggaccaaac gtgtaatata
     4081 atagactccc tgtattgatg gtgatctatc ggaagaccaa tggcgacgct gtcagaagat
     4141 gatgtgcttg agcaactgga tgcacaggac aatttatttt catttatgaa aactgcgcat
     4201 tctattttgc tccaggggat acgccagttt ctgccgtcgc tgtttgtcga taacgatgaa
     4261 gagagcgtcg aatatgcagt gaagccgtta ctcgcccaaa gcggcccgct tgacgatatt
     4321 gatgttgcac tgcgtttgat ttatgcgctg gggaaaatgg ataaatggct gtacgccgat
     4381 atcacgcatt tttcccagta ctggcattac ctgaaagaac aggatgaaac gctcggattt
     4441 gccgatgaca tgacttggga ttttatcagc aacgtcaata gcataacctg caatgcgacg
     4501 ctctatgacg cgttgaaagc gatgaagttc gccgatttcg ccgtctggtc agaggcgcgt
     4561 tttagcggaa tggtcaaaac ggcgctgacg ctggcagtaa cgaccacctt aaaggaatta
     4621 acgccgtgaa aattgaatta acggtgaatg ggctgaacgt gcaagcgcag taccatgatg
     4681 aagagataga acgcgtgcat aaaccgttgc tgcgtatgct ggcggcctta cagacggtaa
     4741 atccgcagcg ccgaaccgtg gtttttcttt gcgctccgcc ggggacgggt aaatctaccc
     4801 taaccacctt ctgggcatat ctcgcgcaac aagacccgga gctgcccgct attcagacgc
     4861 tcccgatgga tggttttcac cattacaata gctggctgga tgcgcatcaa ttgcgcccct
     4921 tcaaaggcgc accagagaca tttgacgttg cgaaactggc ggaaaatctg cgccaggtcg
     4981 tggaagggga ttgtacgtgg ccgcagtacg atcgacaaaa gcatgatcct gttgaagatg
     5041 cgttgcacgt taccgcacca ataatcatcg tcgaaggaaa ctggctattg ctggatgacg
     5101 ataactggcg ccagctagcg caattttgtg atttctcgat ctttatcaat gcgccagcta
     5161 ctgcgttgcg ggaacgactg gtggggcgca agctggcggg agggttatcg ctggcagacg
     5221 cagaagcttt ttatgaccgt accgacgggc cgaatgttcg ccgggtgctg gaagagagtc
     5281 tgcctgcaaa tttgacctta atgatgaccg ctacgggtga atatcgtctg gtggattaat
     5341 gttcctgatt agaccatctt ccagtcgcac cacacgagag aaatgacgcc gggtaaatgc
     5401 ggcgtcatga ctgattgcta cgacgctcgt gcctcgctgg cggcacttct ccagccagct
     5461 ttcaaacacg ctcaaccaat tttcgtcaaa atcccgactc ggctcgtcca gcagcaatag
     5521 tggcggagag agtgcttcca ggcaggcgac ggcgaccatc cgtcgctgcg cactatgcag
     5581 atccaacggg tgcgcgctgg cagtgtctgc taactgacaa cattgcaggg cagcatgggt
     5641 acgttgagtg atttcatccg ccgggcattt ttgcagtttc aaaccaaatg cgatctcgtc
     5701 agcgacggtg ctgtgaaaaa gttggttttc cgcttcctga aacagtacgc caatctttgc
     5761 cgcgcgctgg cggtttttca ggtttttcat cgcctgttgt tgcagcataa ccgtaccggc
     5821 tgtgggcgta agaagacctg ccattactcg taacagcgtc gatttccctg cgccattgtc
     5881 gccggtcaac gccaaccatt caccttgttt gagttgcaga gaaatgtcgc aaagacaatc
     5941 cgttgccgcg ccaggccagc gataagatat ctgatttaac gttaacatag tggctggagc
     6001 gctccgtttt gcaattgcca cgcttgctgg cagtagttca ggaaaggtgt gcgatgacgt
     6061 tcaaaaagaa taatcagcga accacgttca aacgcccagt gttgtaagcg ctgcaacagc
     6121 atttcgctgg cctgcggcgt caggcggcta aaggcttcat caagaattaa tagctttggc
     6181 tgcatcgcga tggcgcaggc gatgaccacg cgctgtgtct ctccaccgga gagtgttgcc
     6241 gggtgacgat ggcgcaacgg ctggcactcg gtcagagtca gggcggcatc aatccgcgcc
     6301 ataatctctg cttcagcaag acacagattt tctggcccaa aggcaacttc ttcttccacg
     6361 ctaaaggtac agccggagag ctgcaaatag ggcgattgct ggacgagctg gatggtggcg
     6421 gattgttcat taagcgggag atgcccaatg ggcgtaccga ggagcgtccc cgtgcctgtt
     6481 atctctcctg gcagaaagtc cggataccag cccgccatca gctgtgccag cgtacttttg
     6541 ccactgccgt tgtcaccaaa gatggcgacc atccccggtt tgacatagtg aaagtcataa
     6601 cagaacggag gatgcgttga gtgtgtcggg caataacgga actgttctaa cgtaaccata
     6661 tccacgctcc gaattctgtc agcatcagca agatcatggt gtaacgcgct acgcgttgta
     6721 gggtgctatc ggctggtgcc cacaacgttg ttcgtcggtt gtggatccga aatgcccgca
     6781 tatccagcgc cgcaccacgg acagcaagat cgtttagcgc attgtgtgtt agcggaatag
     6841 ttaacgcggg catcgcacgt aaacgctgat accagccctc atccagcggc acgccgcggg
     6901 cacgctgcgc ttcatgaata atcgctaatt gccgttttaa ctgctcaacg accaataacg
     6961 gcccggcaaa cagataagcg acgcccggcg gcaggcgaga agcaaacaga gcgcggataa
     7021 aacattgcac ggggacgtac tgcatccaca actgtgaggt tgaaacaatc gccagaatcc
     7081 gcaaccagag cgtaatagca tgcgtccaac gctccggcga acgcggcgtg ccgcttagcc
     7141 attctgttag ccagccaccg tgtaccagcc acagcccggc tcccagcgag aacatcagcc
     7201 agacgacata ttttgcccgc cgccgcgtgg cctttagcgc aataaggcaa aaaaaggtcg
     7261 ccgcgctata gatcggcaaa atggtctgcg cgggcaggat aagcgtggtg caggccgcca
     7321 atgcccatag tgttaatgag gtaaatggat gcatttagcg cacggcagcc attgccggaa
     7381 aatggcgcgt ggtgcgaatc ggcagctggc gcagtaaaag ccagacaatc accgctgtaa
     7441 gagttttatc gaccaggttc gcgcctatca ccgtaatcgc caccgactcc accaggtttt
     7501 gccccatcga gtgcatccag gcaacgaaca aatcggctcc gctaccggtg acaccgccga
     7561 acagcgcagt acgcagcggt acagcgacga ccgttaccgc aagcgtaata atcacgccgc
     7621 tgacaacgac ttttggcagg gtgcgaaacc aacccgctcg cgccagccaa ccggcgacca
     7681 ggccgatgac catcgcgacg ggggcaaaag cggcagcgat cgggtcggtc agtaatcccc
     7741 agagtaaatt ggtcagtaag ccggtcagca tcccgatcac cggaccaagc agtaccgcgc
     7801 taataagtgt gccgatggag tcgagaaata taggtaattt gaccatgctg gccagttgcc
     7861 cgccgatcat attgatggcg atagatataa cgatcaacac cagtgcctga ctggaaaagt
     7921 ggctacgcgc cataagaaaa cctctctgaa agtagttatt taacggggga aatatgcaac
     7981 tcgtcgtaac cagaaagcgc gcaaggttgg cggctaaacg ccagttcctc aaggctaccg
     8041 atcaccagtt caagcgtggt ccgcacggta cagccgggca tcatatgacc gccaagcatc
     8101 gtgccgtgtg gatcggaaac gcagagatga agatgctcac cactctgttc caacgtgccg
     8161 tttagtgaga tcacttcgaa tgtaccgttc aggagcgtgg tgccttcttg cccggcatag
     8221 cgcaaggcga tgtcagtcag gctaccggta cagcctgcaa tccaggcggc atgaagttgc
     8281 tgctgctgtg caaaagcgtg tagctgggaa aaaacttcct gcccggggag gaggcgcagt
     8341 gcgtaaaagc gtgcggagga agaatatggg tagggcaatg tcatcattgg agtactccga
     8401 aactcggacg tattacggat aatatcgacg ctgcatcagt catattctga tataagtcag
     8461 ggtttgtgac ggcacaacga aaagattttg ctacgcttat tcgtgattct gcaaagtgga
     8521 ataaattcca caggggcggg gctgaaaacg tgacgtaagt cagataattg ttttctttga
     8581 ctggacaatc attcctttta ttccacgttt cgcttatcct agctgaagcg tttcagtcga
     8641 ttaaatgttc gacaattaac caatcagtcg cagtttgcga caggtaaggt ttccccggac
     8701 gatttgctgg attactctgt ctggcaaatt tgctcaggga aaaccttgca ggagatctat
     8761 gaccgtacgc gtagcgataa atggcttcgg tcgcatcggg cgtaatgtgg ttcgtgcttt
     8821 gtatgaatcc ggacgccggg cggaaattac cgtggtggca atcaacgaac tggcggatgc
     8881 tgcgggcatg gcgcatttgt tgaaatacga caccagccat ggccgttttg catgggaagt
     8941 acgacaggaa cgcgatcaac tttttgttgg tgatgacgcc atccgcgtat tgcatgaacg
     9001 ttcactgcaa tcgctcccct ggcgtgaact tggcgttgat gtagtcctcg actgcaccgg
     9061 cgtatatggc tcccgcgagc atggcgaagc gcacattgcc gccggggcta aaaaagtgct
     9121 cttttcacat cctggcagta acgatctcga cgcgaccgtt gtttacggcg tcaatcagga
     9181 tcaacttcgt gcggagcacc gcatcgtttc taacgcttcc tgtaccacga attgcataat
     9241 tcccgtcatc aaattgttag atgatgcgta cggtattgag tccggcactg tgaccacaat
     9301 tcactccgcc atgcacgatc aacaggttat tgatgcatac catcctgacc tgcgtcgcac
     9361 ccgggcagcc agccagtcga tcattccggt cgatactaaa ctggccgccg gtatcacacg
     9421 attttttccg caatttaacg atcgctttga agcgattgcg gtacgtgtgc caaccataaa
     9481 tgtgacggca atcgatttaa gcgtgacggt gaagaaacct gtaaaagcca atgaagtcaa
     9541 cctgttgctg caaaaagcag cacaaggtgc atttcatggt atagttgact atacggaatt
     9601 gccgttggtc tctgtagatt ttaaccacga tccgcacagt gccattgtcg atggcaccca
     9661 aacccgggtc agtggcgcac acctgatcaa aacgttggtc tggtgcgata acgaatgggg
     9721 ctttgctaac cgaatgctcg acacgacgtt agctatggct actgttgctt tcaggtaaga
     9781 cgcaagcagc gtctgcaaaa cttttagaat caacgagagg attcaccatg tctgtaatta
     9841 agatgaccga tctggatctt gctgggaaac gtgtatttat ccgtgcggat ctgaacgtac
     9901 cagtaaaaga cgggaaagta accagcgacg cgcgtatccg tgcttctctg ccgaccatcg
     9961 aactggccct gaaacaaggc gcaaaagtga tggtaacttc ccacctgggt cgtcctaccg
    10021 aaggcgagta caacgaagaa ttctctctgc tgccggttgt taactacctg aaagacaaac
    10081 tgtctaaccc ggttcgtctg gttaaagatt acctcgacgg cgttgacgtt gctgaaggtg
    10141 aactggttgt tctggaaaac gttcgcttca acaaaggcga gaagaaagac gacgaaatcc
    10201 tgtccaaaaa atacgctgca ctgtgtgacg tgttcgtaat ggacgcattc ggtactgctc
    10261 accgcgcgca ggcttctact cacggtatcg gtaaattcgc tgacgttgcg tgcgcaggcc
    10321 cgctgctggc agctgaactg gacgcgctgg gtaaggcact gaaagaacct gctcgcccga
    10381 tggtggctat cgttggtggt tctaaagtat ctaccaaact gaccgttctg gactccctgt
    10441 ctaaaatcgc tgaccagctg attgttggtg gtggtatcgc taacaccttt atcgcggcac
    10501 aaggccacga tgtgggtaaa tccctgtacg aagctgacct ggttgacgaa gcaaaacgtc
    10561 tgctgaccac ctgcaacatc ccggttccgt ctgatgttcg cgtagcaacc gagttctctg
    10621 aaaccgctcc ggctaccctg aaatctgtta acgacgtgaa agctgacgag cagatcctgg
    10681 atatcggtga tgcttccgct caggaactgg ctgaaatcct gaagaatgcg aaaaccattc
    10741 tgtggaacgg tccggttggc gtgttcgaat tcccgaactt ccgcaaaggt actgaaatcg
    10801 tggctaacgc tatcgcagac agcgaagcgt tctccatcgc tggcggcggc gacactctgg
    10861 cagcaatcga cctgttcggc attgctgaca aaatctccta catctccact ggcggcggcg
    10921 cattcctcga attcgtggaa ggtaaagtac tgcctgcagt agcgatgctc gaagagcgcg
    10981 ctaagaagta aaaaatcaca gggcagggaa acctgccctt gtttcagcgc gcttttagag
    11041 cacgcatttt ttcatactct aaataattcg agttgcagga aggcgacaag agagtgaatc
    11101 cccaggagct tacataagta agtgactggg gtgagcgaac gcagacgcag cacatgcaac
    11161 ttgaagtatg acgagtataa ggcccgacga tacaggacaa gagacatgtc taagattttt
    11221 gatttcgtaa aacctggcgt aatcactggt gatgacgtac agaaagtttt ccaggtagca
    11281 aaagaaaaca acttcgcact gccagcagta aactgcgtcg gtactgactc catcaacgcc
    11341 gtactggaaa ctgctgctaa agttaaagcg ccggttatcg ttcagttctc caacggtggt
    11401 gcttccttta tcgctggtaa aggcgtgaaa tctgacgttc cgcagggtgc tgctatcctg
    11461 ggcgcgatct ctggtgcgca tcacgttcac cagatggctg aacattatgg tgttccggtt
    11521 atcctgcaca ctgaccactg cgcgaagaaa ctgctgccgt ggatcgacgg tctgctggac
    11581 gcgggtgaaa aacacttcgc agctaccggt aagccgctgt tctcttctca catgatcgac
    11641 ctgtctgaag aatctctgca agagaacatc gaaatctgct ctaaatacct ggagcgcatg
    11701 tccaaaatcg gcatgactct ggaaatcgaa ctgggttgca ccggtggtga agaagacggc
    11761 gtggacaaca gccacatgga cgcttctgca ctgtacaccc agccggaaga cgttgattac
    11821 gcatacaccg aactgagcaa aatcagcccg cgtttcacca tcgcagcgtc cttcggtaac
    11881 gtacacggtg tttacaagcc gggtaacgtg gttctgactc cgaccatcct gcgtgattct
    11941 caggaatatg tttctaagaa acacaacctg ccgcacaaca gcctgaactt cgtattccac
    12001 ggtggttccg gttctactgc tcaggaaatc aaagactccg taagctacgg cgtagtgaaa
    12061 atgaacatcg ataccgatac ccaatgggca acctgggaag gcgttctgaa ctactacaaa
    12121 gcgaacgaag cttatctgca gggtcagctg ggtaacccga aaggcgaaga tcagccgaac
    12181 aagaaatact acgatccgcg cgtatggctg cgtgccggtc agacttcgat gatcgctcgt
    12241 ctggaaaaag cattccagga actgaacgcg atcgacgttc tgtaagtcat tcctttctgc
    12301 ttatctcaag gcccgctctg cgggtctttt tttcgccaaa agcaaaattg cctgatgcgc
    12361 tatgcttatc aggcctacac gtatcctgca atatattgaa tttgcaggtt tttgtaggcc
    12421 ggataaggcg ttcacgccgc atccggcatg acaaagcgca cattgatatc tgtctgagcc
    12481 ccgctctgcg gggatttttt tgccaaaagc aaggtgattc agatgagaaa tctgtgatct
    12541 atttggcaaa attatgcttt attgtttacc cttgtcagac tgcccgtcat aaggcggcgg
    12601 agtgtatttc tccattttga gtcagttgaa aaggaatatt gaatggaaga tttgaatgtt
    12661 gtcgatagca taaacggcgc gggaagctgg ctggtagcta accaggcgct gctgctaagt
    12721 tatgcagtaa acatcgtggc ggcactcgcg atcatcatcg ttggtttgat tatcgcgcgg
    12781 atgatttcca acgcggtgaa tcgcctgatg atctcccgta aaatcgatgc cactgttgct
    12841 gattttcttt ctgcattagt ccgttacggt attatcgcct ttacgctaat cgcagccctg
    12901 ggacgcgtgg gtgtacaaac agcgtcagtc attgctgtac tcggtgccgc aggcttagct
    12961 gttggtctgg ctttgcaggg gtcactttct aacctggccg ctggcgtgtt acttgtcatg
    13021 ttccgcccgt tccgtgccgg agaatatgtt gacctcggcg gcgtagccgg tactgtgctg
    13081 agtgtgcaga ttttctccac caccatgcgt actgcagacg gtaaaattat cgttattccg
    13141 aacggtaaaa ttattgccgg aaatattatt aacttctccc gcgagccagt tcgccgtaac
    13201 gaatttatta ttggcgtggc gtatgattcc gatatcgatc aggttaagca gatcctgacc
    13261 aatattatcc agtctgaaga tcgcattttg aaagatcgcg aaatgactgt gcgcctgaac
    13321 gaacttggtg catcgtcgat taatttcgtg gtccgcgtct ggagcaacag cggcgatctg
    13381 caaaacgtgt actgggatgt gctggagcgt attaaacgtg aatttgatgc cgccggtatc
    13441 agcttcccgt acccgcaaat ggatgtgaac tttaagcggg tgaaagaaga caaagctgcg
    13501 taatcaacgc tgaggccaga taatactctc tggcctctct cttattagtt attctgattg
    13561 ccaattaata ttatcaattt ccgctaataa caatcccgcg atatagtctc tgcatcagat
    13621 acttaattcg gaatatccaa cgtgttttct tattactttc aaggtcttgc tcttggggca
    13681 gctatgattc tgccgctcgg cccacaaaat gcttttgtga tgaatcaggg cattcgtcgt
    13741 cagtaccaca ttatgattgc cttactttgc gctatcagcg atttggtcct gatttgcgcg
    13801 gggatttttg gtggtagcgc gttattgatg cagtcgccgt ggttgctggc gctggtcacc
    13861 tggggcggcg tagtcttctt gctgtggtat ggttttggcg cttttaaaac agcgatgagt
    13921 agtaatattg agttagctag tgccgaagtc ctgaagcaag gcagatggaa aattatcgcc
    13981 accatgttgg cagtgacctg gctgaatccg catgtttacc tagatacttt tgttgtactg
    14041 ggcagccttg gcgggcagct tgacgtcgaa ccaaaacgtt ggtttgcgct cgggacaatt
    14101 agcgcctctt tcctgtggtt ctttggtctg gctattctcg cagcctggct ggcaccgcgc
    14161 ctgcgcacgg caaaatcaca gcgcattatc aatctggtag tgggatgtgt catgtggttt
    14221 atagccttgc agctggcgag agacggtatt gctcatgcac aagccttgtt cagttaggcg
    14281 ctatctgatg gaaaaataaa acagaggcgc taagcttgcc tccagagatc ctgaattttg
    14341 ggcaagtcaa ttaacacgga gagactaacg tgaagttcaa agttatcgcc ctggcggcat
    14401 taatgggtat tagcgggatg gcagcgcagg ctaacgaatt gccggatgga ccgcatattg
    14461 tcacctccgg tacggcaagt gtggatgcgg tgccagacat tgccactctt gcgattgaag
    14521 ttaacgtggc cgcgaaggat gccgctacag ccaagaaaca ggcagatgag cgcgtcgcac
    14581 aatacatttc cttccttgaa ctcaatcaga tcgcgaaaaa agatatcagc tcagcgaatt
    14641 tacgcaccca gccagattat gattatcagg atggtaaaag tatcctgaaa ggctaccgcg
    14701 ctgtgagaac ggtggaagtc acgctccgtc agttagacaa actgaattcc ttgctggatg
    14761 gcgcgctgaa ggcgggtctt aacgaaattc gttcagtgtc gctgggcgtg gcgcagccgg
    14821 atgcctataa agacaaagcg cgtaaggcag cgattgataa cgcgattcat caggcgcagg
    14881 aactggcgaa cggctttcat cgtaaactgg ggccggtata tagcgtgcgc taccatgttt
    14941 ccaactatca gcccagccca atggtgcgga tgatgaaagc cgatgccgcg ccggtgtctg
    15001 cccaggaaac ttacgagcag gccgctattc agtttgatga tcaggtcgat gtggtcttcc
    15061 agttagaacc tgtggatcaa caacccgcta aaacacctgc agcacaataa tacttgtagc
    15121 ccgacgtatt cgtcgggcta atgcctttac ccgatattgc ctcctttttc tgtaattttc
    15181 tgtaaaacca tgacgtaatc acctatcttt gctcgcggtt tcatcaagga taggctgact
    15241 gaaacggtta agcggacaag gatgcgggga ggggggatgg acatttttat ttcgaaaaag
    15301 atgcgcaatt tcattttatt agcgcaaacc aataatattg cccgggcggc agaaaaaatc
    15361 catatgacgg cttcgccatt tggtaaaagc attgccgcgc tggaagaaca aattggctat
    15421 acgctattta cccgcaaaga taataatatc agcctcaata aagccggtca ggaactgtat
    15481 caaaaactgt tcccggttta tcaacgactt tctgcgattg ataatgaaat ccataactca
    15541 gggcgtcgtt cacgagaaat tgtgattggc atagataata cttatccgac aattattttc
    15601 gatcagttga ttagtctcgg agataagtat gaaggcgtga ccgcccagcc agttgagttc
    15661 agcgaaaacg gcgttatcga taatcttttt gaccgtcagt tagattttat tatctcccca
    15721 cagcatgtat ctgcacgagt tcaggaactg gaaaacctga ctatcagtga attaccaccg
    15781 ctgcgattga actttctcgt ttctcgccgt tatgaagagc gtcaagagca agagctgctt
    15841 caggagctgc cctagctaca aatgcgcttt caaaaccggg ctaacttcga agcaatgatt
    15901 gatgcgaata tgcgaccttg tgggatcaat cccaccatta tttatcgtcc ctacagtttt
    15961 atggccaaaa tcagcgccgt ggagcgcgga cattttctga cggtgatccc gcattttgcc
    16021 tggcgactgg tgaatccggc aacgctgaaa tattttgatg cgccgcacag gccgatgtat
    16081 atgcaggaat atctttattc aatcagaaat catcgttata ccgccacgat gcttcagcat
    16141 attgctgaag atcgtgacgg gacaaaccat taacccagca tcgagccggt tgcaattaaa
    16201 ttacggtgta agtcgaagac gtggctaaga tcgtggtgaa tatgtccgcc gggcgcattt
    16261 tccagatagc gatgcagata atcccgatac ataggatgtg cacaattatc aatgatagtg
    16321 cgggcgcgtt gaagcgggga aagaccgcgc agatcggcga tcccttgttc ggtgatgatc
    16381 actttgacgc tgtgttcgtt gtgatcaaca tggctgcaca ttggcacgac ggttgagatc
    16441 ttgccttctt tagcaatcga cggagccata aagatcgaca gatacgcgtt gcgttcaaaa
    16501 tcaccgctgc cgccgatgcc gttcatcaga tcgaccccgg ctacgtgcgt tgagttggca
    16561 tgcccgtaaa tatcaaactc caggccgacg ttcagagcga tgacgcccag acgacggatg
    16621 atttccgggt tattggaaat ctcctgcgga cgcaacacaa tgcggctggc aaagtaatcc
    16681 atattgtcgt aaatcttgcg cagggaatcg gccgagattg tcaggctgga ggcgctggcc
    16741 ccgctgattt tgccggtttc cagtaaatgc accaccgatt cctgtagcac ttccgaatac
    16801 atcataaacg gaggaatttc cgggttttcc cccagacgcg ccattaccgc attattgata
    16861 ttgcccacgc cactttgcag cggcagaaat tccggcggaa tacgcccatg cgccatttcc
    16921 tgcaataaga acgtgaccac gttatcggca atctgctggc acatgggatt ttgcttatcc
    16981 agcatattac cggcgtcggg caagttggtt tccacgacgg cgacaatctt tttcggatcg
    17041 atttgcacat agcgggtacc gacgcgatcc attgcatgga agatcgacac gctattgcgc
    17101 cgcggtggcg cgccaggaat cacaatatcc gccagttctg caacgcgcgg atcgtgatag
    17161 tgattgagtt caatgatcac tttcttcgct cgcagcagcc aggtcggcgc attaccgatc
    17221 ccgctggtta accagactcg accatctggt gccagtgccg atgcttcaat gacggcaaca
    17281 tcaatgtcgc cgaagaaacc gtaattgacc atttgcgcca cttcgctcaa atgcaggtca
    17341 acgaaactca ccgcgccctg attgatcttt ttacgtaaac cggacgatgt ttgatatggc
    17401 gcacgccagg aaacagcatc ggcgtcagaa agtacatcgt cagcggcggc gctgattgac
    17461 gcccccgtca gcaggcgaat ttgatacggc tttttggcct catgctgttc gttagctctg
    17521 cgggcaatcg cggtgggtag ggctttcggc gaacccgctg gggtaaagcc gctaaatgcc
    17581 accatgtcgt tatgctggat aatttctgcc gcttcatcgg cggtcatcct tgtccactga
    17641 gtttccattt ttactccttc gttcgcaggg attaatgacc aacgaaatta ggtttacgtt
    17701 tttcgaggaa agcgttcatc ccttcctggt aatcttcgct gtcataaacc gcgcggcgca
    17761 tcccctgaat acgttcaaat tcatcggagt tcatggtgtg tgcttcgccc agtacacgca
    17821 gctcttcttt gataacggca atggctaacg gcgctttctc agagatgtgg tgcgccattt
    17881 gtaaggtgaa atcttccagt tcttccactt ccacaacatg gttgaggatg ccgacagcca
    17941 gcgcgcgctg ggcggtgatg ggcgaagcgg taaaaatcag ctctttgaca atgtggaagc
    18001 ccgcgtcgcg ggtcaggttg tgaatgccga ccaggttata cgggacgccg aggtttacag
    18061 gcgtcattga gaaggttgag gtactggcgg cgataatcag atcggaactc atgatcattt
    18121 caaatgcgcc accccaaaca ctaccttcca ccatcgaaat aatcggtttc gggaattttt
    18181 ggatcatgcg ggtgatttga cgcaatggat catcatagga gagcggatcg cgaccgccag
    18241 acggcagttc gtgaatatcg tgacctgcgg agaagacttt ggatccactc ggcgcgcgca
    18301 aaatgataca gcgaatttcc ggccggttga gatcgcttaa cgcctgcata agatcatcaa
    18361 taaagacttt acttaaggca ttaagttttc ggccatagtt aaactcaatg accgccactt
    18421 tgttgatagt gacaacgtta acatactgat aagacataaa aattccttta atcaaaatat
    18481 tgcgtctgga taaattcact gagctgccgc aggccggtgc gcggtgagag cgtattgttt
    18541 ttgaccgcta aaagcgtctg gcggtaatag cgatcgaaat cttcattcgc gaacaggtga
    18601 ttcagtactt cttcttcggt ctgcttacgc agccattcca ccgattgttg ttgccgcact
    18661 tgttgtaaac gaccactggc agttagcgcg gttttgaagt cgatgatggc gtgccagatc
    18721 tcatcgattc cacgtttttc cagtgcgcta caagtcagaa cccgtggctg ccattcgtcg
    18781 tatttacgtc gcagaatatg cagggcactc tcgtacatat gccgggcaat ggcgacattg
    18841 gtatggttat cgccatcgtc tttgttgata acgatcagat cagccacttc catcagccct
    18901 tttttaatgc cctgcagatc atcgccgcca ccggcaattt gcaacgagat aaaacagtcc
    18961 accatgcggg cgacttctgt ttccgactgc ccgacgccaa ccgtttcgac aatcactacg
    19021 tcataacccg ctgcttcgca taacagcatt aattcccgcg ctcgctgact ggcaccgccc
    19081 agatgaccgg aggatggtac cgggcgaata aacgccgctt cggcacgcgc caggtcattc
    19141 atgcgggttt tatccccgag aatgctaccg ccagtgaccg ggctgctggg atcgaccgca
    19201 ataaccgcga cctttaatcc ctctcgaatc aacaacatgc caaaggcctc aagaaaggta
    19261 cttttccccg cgccgggggt gccggtaacg cccagtcgca gggtgttacc gcagtacggc
    19321 ataatggcat caagcagctg cgtacttagt gcctgatgac gcgggtgacg gctttccacc
    19381 agcgtcatgg cctgggcgag tgtggcatgc tcaccctgac gtaagcggcg aatactttct
    19441 gccagcgtgg cttcattaat catgatgctg gcttatcaga ttcagtacgt cgcgcacact
    19501 gtcgagcata ggtgtacctg gaccataaat cgccgccacg ccgcgctctt gcaggaaggc
    19561 gtaatcctgc ggtggaatga cgccaccggc gactacgcag atatcttcgc gtccccattt
    19621 tttcagcgct tcgaccagtt ccgggatcag cgttttatga ccggcagcca gtgaggatgc
    19681 gcccactacg tgaacgtcat tttctactgc caggcgggcg atctcttcag gtgtagagaa
    19741 catcgggctt aaatctacgt cgaaaccgag atcggaatag gcgctggcga tcactttcgc
    19801 gccgcgatcg tgtccatcca ggcccatttt ggcgatcaga atgcgcgggc gacgaccatt
    19861 gtcggcaagg aactgctccg tttgcgcaac aatggcatcg aactcggagg ccgatttctc
    19921 agattgatga tagctttgcg caatcacgcc ggtaacacac tggcttggca ccagataacg
    19981 gtcgaaagcg gcttccagcg catcggaaat ttcacccagg gtggcgcgaa cgcgagcggc
    20041 attaacagcg gcagccagca ggttttcgtt atgctgtgcg gcgtgagtca gggcgttcaa
    20101 cgcggcggtt acggcggcat catcacgggt ggcgcgaatg cgttccagcg aagcaatttg
    20161 ctcgttacgc accatcacgt tgtcgatctc aagtacatcg gtttcgtctt cgtgatccag
    20221 tttgtacttg ttgacaccaa cgatgacacg cttgccctgg tcgatcagcg actgttcgcg
    20281 cgctgaggcc tcttcgatca ttcgttttgg cagacctgct tcgatcgctt tcgccatgcc
    20341 accggcttcg tcgatctgtt ggataatagc tctggcttgt ttgacgattt gatcggtcag
    20401 cgactcaatg taataggatc cggccagtgg atcgacggtg cggcagagtt ctgattcttc
    20461 ctggatgatg atctgggtgt tgcgggcaat gcgtgctgag aaatcggtag gcaaaccaag
    20521 cgcttcgtca aaggcgttgg tatgcagtga ctgagtaccg cccagcgtcg cagccagcgc
    20581 ttcaatggtg gtgcggataa cgttgttata cggatcctgt tcagtcaggc tccagcctga
    20641 ggtctggcag tgggtacgca gcgccagtga tttcgggtcc tgtgcgccaa atccactgac
    20701 cgcttcgctc cataaataac gtgccgcacg caacatggcg acgttcataa acagatccat
    20761 gccgatgccg aagaagaacg acaggcgagg agcgaagtca tcaattttca gtccggcaga
    20821 gattgctgct ttgatgtact caatcccatc agcgagcgta aatgctacct gctgcacgca
    20881 gttggcaccc gcttcaccca tgtggtaacc gctgatactg atggtattaa atcgcggcat
    20941 gttgccggaa caccaggcga tgatgtcggc gataatgcgc attgacggtt ttggcgggta
    21001 aatataggtg ttgcggcaga ggtactcttt gagaatatcg ttttgaatgg tgccggtcag
    21061 tttatcaggt gtaacacctt gctcttccgc ggcgatgata taaaacgcca gtactggtag
    21121 cactgcgcca ttcatggtca tcgaaaccga cattttatcc agcgggatct ggtcgaacag
    21181 gactttcata tcttccacgg tgtcgatagc gacgcccgtt ttgccgacgt cacccgccac
    21241 gcgtgggtta tcggagtcgt agccacggtg ggtggcaagg tcaaacgcaa cggaaagacc
    21301 tttttgcccg gcggccaggt tacggcgata aaaagcgttg gactcttttg ctgttgaaaa
    21361 accagcatac tgacggatgg tccacggttg ggcggtatac atagtggcac gcgggccacg
    21421 aacgtagggc ggcaaaccag gaagggtacc tgtcacctcc agattatcga gatcggcttc
    21481 ggtatacagc ggcttgatgg cgatcccttc cgcggtttgc tgaaccagcg agtcgacagt
    21541 tttctcccga cggctcaatt ccttgttggc aagctgttgc cactcctgct cgttagacat
    21601 tatttgctcc gtaaaacgca atcaaaatga gggtatttgc gcttacggtg agctttttac
    21661 gtgggtttgt cgccagcaaa aaaaatgcat tcagacgtcg ttttttgccg cctgagattt
    21721 gtctattttg tgatgtgatt caaggaatac aaaaagaagt gaggataacg cctgatatgt
    21781 gctatcaggc attatttgat ggattaatcc tgacgaagga ctttgtgacc ataatcgagc
    21841 aacgcatcag tgactttacg catcatgcgg ctttcaggag caaagcggtg ccagtagagc
    21901 atccggcgtt gaaatagccc tggcgttaag tcaatcagtt caccgctggc tagttctttc
    21961 tcgatttgca ggtgcgggat catacagcag gtggtgccct ggcgagcaag ttgcacgaac
    22021 gcttctgaag aattaacgat atggcagggc acgctgcctg gaggcagatc gaagttttgc
    22081 tgcaaaaagg cctggtgcat atcgtcaaga tggtcaaacg cgaccactgg cgctttcagt
    22141 aatgccgaac gcgttacgcc gttagggaaa tatttttcgg caaagggttt tgagctgacg
    22201 aacagatagt cgagcgcacc aagtttatcg acaagacaac tcggcagcgc ctgatgttga
    22261 atactcaccg cgccgaccac ttcgccgcga cgcagacgtt cctgagtgcg ggtttcatct
    22321 tctacctgca agttgaggcg gataggcgaa tcagccaata caggagccag tgccggaagc
    22381 aaccacgtcg ccagactgtc ggcattgacc gccagtgaaa gcagcagcgg agtcgaaccg
    22441 gtttgttcat cgcccagcca ctcttcttcc agcaactcca cctggcgcag cagtgccagc
    22501 agtttttgcc cttgttccgt cgggcgcggc ggtacggtac gcaccaacag cggctgcccg
    22561 aacatatttt ccagttgctt aatgcgctgt gagacggctg attgtgtaat gcacagcttt
    22621 tgtgccgcgc gctcaaatcc tcgttcacgt atcaccgcat ccagtgcctg taatgttctg
    22681 tagtccgggc gtttcattgc tgttgtctgc tcctggtaaa tgctttttcc tgcactatga
    22741 cacaattttg tgtcaggttg catataccgc agccgtcggc gggtaacatt tgtatcaatg
    22801 gttaaacgcg cagcttaaaa gatcagggat ttgcgctatt gcatttcgct tagcaacttc
    22861 gcgccctggt tatgttgaat ggcgtggcgt tattgcctct gttcgcaccg tggagcggca
    22921 tacccagacc gttgtatgct ttatttgcgg ggcgaaaggg gatgcccgcc attgcgcgat
    22981 attttatgga tgagttaacc acgcggcttg ccaacggggt ctgaatcgct ttttttgtat
    23041 ataatgcgtg tgaaatttca taccacaggc gaaacgatca tgacgcagga tgaattgaaa
    23101 aaagcagtag gatgggcggc acttcagtat gttcagcccg gcaccattgt tggtgtaggt
    23161 acaggttcca ccgccgcaca ctttattgac gcgctcggta caatgaaagg ccagattgaa
    23221 ggggccgttt ccagttcaga tgcttccact gaaaaactga aaagcctcgg cattcacgtt
    23281 tttgatctca acgaagtcga cagccttggc atctacgttg atggcgcaga tgaaatcaac
    23341 ggccacatgc aaatgatcaa aggcggcggc gcggcgctga cccgtgaaaa aatcattgct
    23401 tcggttgcag aaaaatttat ctgtattgca gacgcttcca agcaggttga tattctgggt
    23461 aaattcccgc tgccagtaga agttatcccg atggcacgta gtgcagtggc gcgccagctg
    23521 gtgaaactgg gcggtcgtcc ggaataccgt cagggcgtag tgaccgataa cggcaacgtg
    23581 atcctcgacg tccacggcat ggaaatcctt gacccgatag cgatggaaaa cgccataaat
    23641 gcgattcctg gcgtggtgac tgttggcttg tttgctaacc gtggcgcgga cgttgcgctg
    23701 attggcacac ctgacggtgt caaaaccatt gtgaaatgat ctgacggggg aacctccccc
    23761 gttaaaaaaa ttctcttcat taaatttggt gacatgtgtc acgtttttac ctggatattg
    23821 tcgattgctc taaataaatc ctctaaacca gcatattcaa ccaagaaata cctttgcgtg
    23881 atatttcctc aacatcgcga cgcaaacgtt catattgccg caatattatt ttttgatatg
    23941 ttgaaaggcg gatgcaaatc cgcacacaac atttcaaaga caggattggg taaatggcaa
    24001 aggtatcgct ggagaaagac aagattaagt ttctgctggt agaaggcgtg caccaaaagg
    24061 cgctggaaag ccttcgtgca gctggttaca ccaacatcga atttcacaaa ggcgcgctgg
    24121 atgatgaaca attaaaagaa tccatccgcg atgcccactt catcggcctg cgatcccgta
    24181 cccatctgac tgaagacgtg atcaacgccg cagaaaagct ggtcgctatt ggctgtttct
    24241 gtataggaac aaaccaggtt gatctggatg cggcggcaaa gcgcgggatc ccggtattta
    24301 acgcaccgtt ctcaaatacg cgctctgttg cggagctggt gattggcgaa ctgctgctgc
    24361 tattgcgcgg cgtgccggaa gccaatgcta aagcgcaccg tggcgtgtgg aacaaactgg
    24421 cggcgggttc ttttgaagcg cgcggcaaaa agctgggtat catcggctac ggtcatattg
    24481 gtacgcaatt gggcattctg gctgaatcgc tgggaatgta tgtttacttt tatgatattg
    24541 aaaacaaact gccgctgggc aacgccactc aggtacagca tctttctgac ctgctgaata
    24601 tgagcgatgt ggtgagtctg catgtaccag agaatccgtc caccaaaaat atgatgggcg
    24661 cgaaagagat ttcgctaatg aagcccggct cgctgctgat taatgcctcg cgcggtactg
    24721 tggtggatat tccggcgctg tgtgatgcgc tggcgagcaa acatctggcg ggggcggcaa
    24781 tcgacgtatt cccgacggaa ccggcgacca atagcgatcc atttacctct ccactgtgtg
    24841 aattcgacaa cgtccttctg acgccacaca ttggcggttc gactcaggaa gcgcaggaga
    24901 atatcggcct ggaagttgcg ggtaaattga tcaagtattc tgacaatggc tcaacgctct
    24961 ctgcggtgaa cttcccggaa gtctcgctgc cactgcatgg tggtcgtcgt ctgatgcaca
    25021 tccacgaaaa ccgtccgggc gtgctaactg cgctgaacaa aatcttcgcc gagcagggcg
    25081 tcaacatcgc cgcgcaatat ctacaaactt ccgcccagat gggttatgta gttattgata
    25141 ttgaagctga cgaagacgtt gccgaaaaag cgctgcaggc aatgaaagct attccgggta
    25201 ccattcgcgc ccgtctgctg tactaattcc ccttctctga aaattaacgg gcaggtcact
    25261 gacttgcccg tttttttatc ccttctccac accgttgccg cgttgcgcat tctactttgc
    25321 gagtctgctc cagaaaataa atcaaagtca gatcaatgcg ttgtgtttat ttaatgggga
    25381 tttacgatgg cgggacagca tggggctgta acggtgaagc cctcaccgaa gcgagggctt
    25441 aaaggagaag ggttatgatg cgacttgtca tcatactgat tgtactgtta ctgataagtt
    25501 tcggagctta ttaacagtca gtctcagggg aggagtcatc ctcccttacc cttactcact
    25561 aaattaggtc aaagaatcaa cgatgtcaat cagggcgatg cggggtgcat cgccctgaac
    25621 cgaacttttt gatagccact tcatcgccca cgtttactgc cgtaattaat cccttattcc
    25681 tgcaatgtat gtctgcctta catgacgttt tacgagtgcg ctcgaagaaa agatttaaag
    25741 gtagatcaat gcgttgtggt tatttaaaga gagtttacga tggcagggca gcatggggct
    25801 gtaacggtaa agccctcacc gaagcgaggg cttgaaggag aagggttatg atgcgacttg
    25861 tcatcatact gattgtactg ttactgataa gtttgccagc ttattaacag tcagtctcag
    25921 gggaggagtc atcctccctt acccttactc actaaattag gttaaagaat caacgatgtc
    25981 aatcagggcg atgcaggtgt atcgccctta ccactcccag actttcgacg gtgtaaccac
    26041 cgcaggaaga gggatatccc actcttcaac ggggagtttt tccaccaact gacaatcatg
    26101 cgcataaccc accggttgcg ttttatagtg ctgccagttt tgtaaggtcc gatcataaaa
    26161 accaccgccc attcccaggc gctgaccgta ctcatcaaag gcgaccagcg gtgtgatcag
    26221 cacgtctaat cgggaaaggg ggagcacgtc acgcacatcc agttttggct catggatttt
    26281 caacctgttc atcaccagtt cgctttgcgg atggtaattc aggaacagca aattaccggc
    26341 actaaaggga tgcaaaactg gaagatatac gcgcttaccg gcgcgccaga gttgttctat
    26401 aagcggctgg gtgtcgagtt caccatcaaa agagaggaat acagcgaccg tatgcgccat
    26461 caccaccggg ggataagtca tcatccgggt agcggcttgt tgacccattt cctgctgttg
    26521 ttccggcgtt aacgcacgac gacgttgccg aatcattttg cggatttctt gtcgggataa
    26581 tgtcagtggg agttctggta gttgcgtcat ggctggtgcc agataagaag ggaatctccg
    26641 agatgccgcc gcaggctgta acccttgaac ccttggttca aggtgaatgt gtcgtcgcag
    26701 ttttaaggct tctcggacgg accgagcatg ctcaccaacc gcggagcgcc acattcttgt
    26761 ggtatgaaat atcggctcag gggactggcc cgcttgcgaa catctcagag aaattttgtc
    26821 ttcacggtta ctctaccaca gtaaaccgaa aagtgtcatt caaagttttg gttagttttt
    26881 tcggtgatgc gaccttgttc aagtaacgct tgttctatgg tctgctgcag catccgaata
    26941 cgctgttcca tacttgccgc gtagtcacga gtcttcgctt tttcttgcgc taactcatag
    27001 ctgatattca atgcggcaat gaagaccaac tgttcagtat ttgtgactct agtgcgttct
    27061 ttcagatctt gcaaccgttg gttcagatcg tccgctgcct gattcaacgc atccctttgg
    27121 tcaggcgggc agttcacacg cagtgaacgg ccaaaaattt ggatatcgac gggttgtgca
    27181 gacatgccac cttcctgctg attgactgcg ctgccttcgt ctttagaccc atggtctgcg
    27241 aaggggcgac actatagcta ccctgatgag aagagacaag cccttttctg gtccaccagg
    27301 gcccaaagtg gtagcatatc atgaatattc ctccctttga cgacgaatgc ttatgtctat
    27361 acagaacgaa atgcctggtt acaacgaaat gaaccagtat ctgaaccaac aagggacggg
    27421 tctgacccct gctgagatgc atggtttaat cagtgggatg atatgtggcg gtaacgatga
    27481 cagctcatgg ctaccgctac ttcacgacct gacgaacgaa ggcatggctt tcggtcatga
    27541 gctggcacag gcattgcgta aaatgcactc tgccaccagc gatgccctgc aggatgacgg
    27601 cttccttttt cagctttatc tgcctgatgg cgatgatgtc agcgttttcg atcgggctga
    27661 tgcattggca ggttgggtca atcacttcct gcttggtctt ggtgttacgc aaccgaagct
    27721 ggataaagtg accggcgaaa ccggtgaagc tatcgacgat ctgcgtaaca ttgcgcaact
    27781 gggttacgac gaagacgaag atcaggaaga gcttgaaatg tcgcttgaag agatcatcga
    27841 atacgttcgt gttgccgcgc tgttatgcca cgacaccttt actcatccgc aaccgaccgc
    27901 gccagaagta caaaaaccga ctctacacta aaaacaaaaa acgtaaggag agtgttatga
    27961 gtgagatatc ccggcaagag tttcagcgtc gccgtcaggc cctggtggag caaatgcaac
    28021 ccggcagcgc cgcgctgatt tttgctgcac cagaagtaac acgtagcgcc gacagcgaat
    28081 acccctatcg tcagaacagt gacttctggt acttcaccgg ctttaacgaa ccggaagcgg
    28141 tgttggtgct gattaaaagc gatgacactc ataaccacag cgttctgttt aaccgcgttc
    28201 gcgacctgac ggcggagatc tggtttggcc gtcgcttagg ccaggatgcc gcgccagaga
    28261 aactgggcgt tgaccgcgca ctggcattca gcgaaattaa tcagcaactt tatcaactac
    28321 ttaatggtct ggatgtggtt taccatgctc agggcgaata tgcatatgct gatgaaatcg
    28381 tgaacagtgc gctggaaaaa ctgcgtaaag gttcgcggca aaatctcacc gcaccggcaa
    28441 cgatgatcga ctggcgtcct gttgttcatg aaatgcgcct gttcaaatcg ccagaagaga
    28501 ttgccgtact ccgccgcgcg ggagaaatca ccgccatggc acatacacgg gcgatggaaa
    28561 aatgccgtcc gggaatgttc gagtaccatc tggaaggcga aattcaccac gaatttaacc
    28621 gccacggtgc gcgctatccg tcctataaca ccattgtagg cagcggtgaa aacggctgca
    28681 ttttacacta caccgaaaac gagtgtgaaa tgcgcgacgg cgacctggtg ttgattgacg
    28741 caggctgcga atacaaaggt tacgcaggcg atatcacccg taccttcccg gtcaacggca
    28801 aattcaccca ggcccagcgc gaaatctacg acattgtgct ggagtctctt gaaaccagcc
    28861 tgcgcctgta tcgtccggga acctccattc tggaagtcac cggtgaagtg gtgcgcatta
    28921 tggttagcgg cctggtaaaa ctcggcatcc tgaaaggtga tattgatgaa ctgatcgctc
    28981 agaacgccca tcgtcctttc tttatgcatg gccttagcca ctggttagga ctggatgtcc
    29041 atgacgttgg cgtttatggt caggatcgct cgcgcattct ggaaccgggg atggtactga
    29101 ccgtagagcc tggtctgtat atcgcgccgg atgcggatgt gccagaacaa tatcgcggta
    29161 tcggcattcg tattgaagac gacattgtga ttaccgaaac cggcaacgaa aacctcaccg
    29221 ccagcgtggt gaaaaagccg gaagaaatcg aagcgttgat ggctgctgcg agaaagcaat
    29281 gagcgtaatc atcgtcggtg gcggcatggc gggcgcgacg ctggcgctgg ctatttcccg
    29341 gttaagtcac ggggcgctgc cggtacattt gattgaagcg actgcgccag agtcacatgc
    29401 tcatccgggc tttgatggac gagcgatagc gctggcggcg ggtacctgtc agcaactggc
    29461 gcgcatcggc gtttggcaat ctctggcgca ttgcgcaact gccatcacca ccgtgcatgt
    29521 cagcgatcga ggtcacgctg gatttgtcac cctcgccgca gaagattacc aactggcggc
    29581 gctgggacag gttgtcgaat tgcacaatgt cgggcaacgg ctgtttgcat tgctgcgtaa
    29641 agcacctggc gtaacgctgc attgccctga tcgcgtggct aacgttgccc gtactcagag
    29701 tcacgttgaa gtgacgctgg agggtggcga gacgctgacg ggccgcgtgc tggtagccgc
    29761 tgatggcacc cattcagcgt tagccactgt gtgcggcgtt gactggcagc aggagcctta
    29821 cgaacaactg gctgtgattg ccaacgttgc gacttccgtt gcgcatgaag ggcgcgcttt
    29881 tgaacgcttt acgcaacatg gcccgctggc gatgttgccg atgtctgacg gacgctgttc
    29941 gctggtctgg tgtcatccac tggaacggcg cgaagaggtg ctgtcgtgga gcgacgagaa
    30001 gttttgccgt gaactccagt cggcctttgg ctggcgactt gggaaaatta cccacgctgg
    30061 taaacgcagt gcttatccgc tggcgttaac ccacgccgcc agatctatta cccatcgtac
    30121 cgtgctggtg ggcaatgcgg cgcaaaccct gcacccgatt gccgggcaag ggttcaacct
    30181 cggtatgcga gatgtcatga gtcttgcgga aaccctgact caggcgcagg agcgcggaga
    30241 agacatgggt gattacggcg tattgtgtcg ttatcagcag cgtcgacaga gcgatcgcga
    30301 agcaaccatt ggcgtcacgg acagccttgt acatcttttt gccaaccgtt ggacgccact
    30361 ggttgtcggg cgcaacatcg ggctgatgac gatggaatta ttcaccccgg cacgcgatgt
    30421 gctggcgcag cgcaccctcg gttgggtggc gcgttgaagg cggttattaa ggaataaaca
    30481 atgcaaagtg ttgatgtagc cattgttggc ggcggcatgg tggggctggc ggttgcctgc
    30541 ggcttacagg gtagcggctt acgcgttgcc gtactggagc agcgcgtacc ggaacctctg
    30601 gcggcggatg caccaccaca actgcgcgtt tcggctatca atgccgccag cgaaaaatta
    30661 ctcacccgtc ttggcgtctg gcaggacatt ctctctcgcc gagccagctg ttatcacggt
    30721 atggaagtgt gggacaaaga cagttttggt cacatctcgt ttgacgatca aagcatgggc
    30781 tatagccatc ttgggcacat cgttgaaaac tcagtgattc actacgcgct gtggaacaaa
    30841 gcgcagcagt cgtcagatat cactcttttg gcccccgcag aattacagca ggtcgcctgg
    30901 ggagaaaatg aaaccttcct gacgctgaaa gatggcagta tgttaacggc gcgtctggtg
    30961 attggcgcgg acggcgctaa ttcctggttg cgcaacaaag ccgatattcc gctgactttc
    31021 tgggattatc agcatcacgc gctggtagcg accatacgca cggaagaacc gcatgatgcg
    31081 gtggcgcggc aggttttcca tggcgaaggc attctggcct ttttaccgct tagcgatccg
    31141 catctttgct cgattgtctg gtcactgtcg ccagaggaag cgcagcggat gcagcaggca
    31201 agtgaagacg aatttaatcg cgcgttaaat atcgcttttg ataatcgcct gggcttatgc
    31261 aaggttgaga gcgcgcgtca ggtgttccca ctgacggggc gttatgcgcg ccagtttgcc
    31321 gcgcaccgtc tggcgctggt gggcgacgcc gcgcatacca ttcacccgct ggcggggcag
    31381 ggggtaaatc ttggctttat ggatgctgca gagctgattg ctgaactgaa acggttgcat
    31441 cgtcagggta aagacatcgg gcagtacatt tatctgcgtc gctatgagcg tagccgcaag
    31501 cacagtgcgg cgctgatgct ggctggtatg cagggattcc gcgatctgtt ttctggtacc
    31561 aatccggtga aaaaactgct gcgtgatatt ggtctgaaac tggccgacac gcttcctggc
    31621 gttaaaccac aacttatccg ccaggcaatg ggattaaacg atttgcctga atggctgcgt
    31681 taaaaatttc tcttctgctg tttatttgat acccatcaca ctttcatctc ccggtttttt
    31741 cgccgggaga ttttcctcat ttgaaataaa ctaatttcac ctccgttttc gcattatatt
    31801 ttctaatgtc acgaattttt gatttagtgt tttttgacat tttttcagct cttaatattg
    31861 ccttattcaa attaactttc tcatcacatc atctttatat agaaacttgt gtattttttg
    31921 gttttttatt ctgttgcgat tttggcgttt tttaaccata agctaatgtg atgatcaatt
    31981 ttaccttatg gttaacagtc tgtttcggtg gtaagttcag gcaaaagaga acgattgcgt
    32041 tggggaccgg gagtggctcc gatgctgggt ttcgtggtga taatttcacc atgaaaaagt
    32101 tgtcagcccc gcttattcaa tgaggacaag atggcacaac agactccttt gtacgaacaa
    32161 cacacgcttt gcggcgctcg catggtggat ttccacggct ggatgatgcc gctgcattac
    32221 ggttcgcaaa tcgacgaaca tcatgaggta cgtaccgatg ccggaatgtt tgatgtgtca
    32281 catatgacca tcgtcgatct tcgcggcagc cgcacccggg agtttctgcg ttatctgctg
    32341 gcgaacgatg tggcgaagct caccaaaagc ggcaaagccc tttactcggg gatgttgaat
    32401 gcctctggcg gtgtgataga tgacctcatc gtctactact ttactgaaga tttcttccgc
    32461 ctcgttgtta actccgccac ccgcgaaaaa gacctctcct ggattaccca acacgctgaa
    32521 cctttcggca tcgaaattac cgttcgtgat gacctttcca tgatcgccgt acaagggccg
    32581 aatgcgcagg caaaagctgc cacactgttt aatgatgctc agcgtcaggc ggtggaaggg
    32641 atgaaaccgt tctttggcgt gcaggcgggc gatctgttta ttgccaccac tggttatacc
    32701 ggtgaagcgg gctatgaaat tgcgctgccc aatgaaaaag cggccgattt ctggcgtgcg
    32761 ctggtggaag ggggcgttaa gccatgcggt ctgggcgcgc gtgacacgct gcgtctggaa
    32821 gcgggcatga atctttatgg tcaggagatg gacgaaacca tctctccgtt agccgccaac
    32881 atgggctgga ctatcgcctg ggaaccggca gatcgtgact tcatcggtcg tgaagccctg
    32941 gaagtgcagc gtgagcatgg cacagaaaaa ctggttggtc tggtgatgac cgaaaaaggc
    33001 gtgctgcgta atgaactgcc ggtacgtttt accgatgcgc agggcaacca gcatgaaggc
    33061 attatcacca gcgggacttt ctccccgacg ctgggttaca gcattgcgct ggcacgcgtg
    33121 ccgaaaggta ttggcgaaac ggcgattgtg caaattcgca accgtgaaat gccggttaaa
    33181 gtgacaaaac ctgtttttgt gcgtaacggc aaagccgtcg cgtgatttac ttttttggag
    33241 attgattgat gagcaacgta ccagcagaac tgaaatacag caaagaacac gaatggctgc
    33301 gtaaagaagc cgacggcact tacaccgttg gtattaccga acatgctcag gagctgttag
    33361 gcgatatggt gtttgttgac ctgccggaag tgggcgcaac ggttagcgcg ggcgatgact
    33421 gcgcggttgc cgaatcagta aaagcggcgt cagacattta tgcgccagta agcggtgaaa
    33481 tcgtggcggt aaacgacgca ctgagcgact ccccggaact ggtgaacagc gaaccgtatg
    33541 caggtggctg gatctttaaa atcaaagcca gcgatgaaag cgaactggaa tcactgctgg
    33601 atgcgaccgc atacgaagca ttgttagaag acgagtaacg gctttattcc tcttctgcgg
    33661 gagaggatca gggtgaggaa atttacacct caccctcact ctcttcgcaa ggagagaggt
    33721 tcacaattca ctgcacgttt caggaaccat cgctcatgac acagacgtta agccagcttg
    33781 aaaatagcgg cgcttttatt gaacgccata tcggaccgga cgccgcgcaa cagcaagaaa
    33841 tgctgaatgc cgttggcgca caatcgttaa acgcgctgac cggccagatt gtaccgaaag
    33901 atattcagct tgcgaccccg ccgcaggttg gcgcaccggc gaccgaatac gccgcgctgg
    33961 cggaactcaa ggctattgcc agtcgcaata aacgcttcac gtcttacatc ggcatgggtt
    34021 acaccgccgt gcagttaccg ccggttatcc tgcgtaacat gctggaaaat ccgggctggt
    34081 ataccgcgta tactccgtat caacctgaag tctcgcaggg ccgccttgag gcactgctca
    34141 atttccagca ggtaacgctg gatttaaccg ggctggatat ggcctctgct tctcttcttg
    34201 acgaagccac cgctgccgcc gaagcaatgg cgatggcgaa acgcgtcagc aaactgaaaa
    34261 atgccaaccg cttcttcgtg gcttccgatg tgcatccgca aacgctggat gtggtccgta
    34321 ctcgtgccga aacctttggt tttgaagtga ttgtcgatga tgcgcaaaaa gtgctcgacc
    34381 atcaggacat cttcggcgtg ctgttacagc aggttggcac taccggtgaa attcacgact
    34441 acactgcgct tatcagcgaa ctgaaatcac gcaaaattgt ggtcagcgtt gccgctgata
    34501 ttatggcgct ggtgctgtta actgcgccgg gtaaacaggg cgcggatatt gtttttggtt
    34561 cggcgcaacg ctttggcgta ccgatgggtt acggtggccc acacgcggca ttctttgcgg
    34621 cgaaagacga atacaaacgc tcaatgccag gccgtattat cggtgtatcg aaagatgcag
    34681 ctggcaatac cgcgctgcgc atggcgatgc agactcgcga gcaacatatt cgtcgtgaga
    34741 aagcgaactc caacatttgt acttctcagg tgctgctggc aaacatcgcc agcctgtatg
    34801 ccgtttatca cggcccgatt ggcctgaaac gtatcgctaa ccgcattcac cgtctgaccg
    34861 atatcctggc ggcaggcctg caacaaaaag gtctgaagct gcgccatgcg cactatttcg
    34921 acactctgtg tgtggaagtg gccgacaagg cgggcgtact ggcgcgtgcc gaagcggctg
    34981 aaatcaacct gcgtagcgat atcctgaacg cggttgggat cacccttgat gaaaccacca
    35041 cgcgcgaaaa cgtgatgcag cttttcagcg tactactggg ggataaccac gggctggaca
    35101 tcgacacgct ggacaaagac gtggctcacg acagccgctc tatccagcct gcgatgctgc
    35161 gcgacgacga aatcctcacc catccggtgt ttaatcgcta ccacagcgaa accgaaatga
    35221 tgcgctatat gcactcgctg gagcgtaaag atctggcgct gaatcaggcg atgatcccgc
    35281 tgggttcctg caccatgaaa ctgaacgccg ccgccgagat gatcccaatc acctggccgg
    35341 aatttgccga actgcacccg ttctgcccgc cggagcaggc cgaaggttat cagcagatga
    35401 tcgcgcagct ggctgactgg ctggtgaaac tgaccggtta cgacgccgtt tgtatgcagc
    35461 cgaactctgg cgcacagggc gaatacgcgg gcctgctggc gattcgtcat tatcatgaaa
    35521 gccgcaacga agggcatcgc gatatctgcc tgatcccggc ttctgcgcac ggcactaacc
    35581 ccgcttctgc acatatggca ggaatgcagg tggtggttgt ggcgtgtgat aaaaacggaa
    35641 atatcgatct gactgatctg cgcgcgaaag ccgaacaggc aggtgataat ctctcctgca
    35701 tcatggtgac ctatccttcc acccacggtg tgtatgaaga aacgatccgt gaagtgtgtg
    35761 aagtcgtgca tcagttcggc ggtcaggttt accttgatgg cgcgaacatg aacgcccagg
    35821 ttggcatcac ctcgccgggc tttattggcg cagacgtttc acaccttaac ctgcataaaa
    35881 ctttctgcat tccgcacggc ggtggtggtc cgggtatggg accgatcggc gtgaaagcgc
    35941 atctggctcc gtttgtaccg ggtcatagcg tggtgcaaat cgaaggcatg ttaacccgtc
    36001 agggcgcggt ttctgcggca ccgttcggta gcgcctctat cctgccaatc agctggatgt
    36061 acatccgcat gatgggcgca gaagggctga aaaaagcaag ccaggtggca atcctcaacg
    36121 ccaactatat tgccagccgc ctgcaggatg ccttcccggt gctgtatacc ggtcgtgacg
    36181 gtcgcgtggc gcacgaatgt attctcgata ttcgtccgtt gaaagaagaa accggcatca
    36241 gcgagctgga tattgccaag cgcctgatcg actacggttt ccacgcgccg acgatgtcgt
    36301 tcccggtggc aggtacgctg atggttgaac cgactgaatc tgaaagcaaa gtggaactgg
    36361 atcgctttat cgacgcgatg ctggctatcc gcgcagaaat tgaccaggtg aaagccggtg
    36421 tctggccgct ggaagataac ccgctggtga acgcgccgca cattcagagt gaactggtcg
    36481 ccgagtgggc gcatccgtac agccgtgaag ttgcggtatt cccggcaggc gtggcagaca
    36541 aatactggcc gacagtgaaa cgtttggatg atgtttacgg cgaccgtaac ctgttctgct
    36601 cctgcgtacc gattagcgaa taccagtaat tcactgattc gactatcttc taaaggcgct
    36661 tcggcgcctt tttagattgt taataaagtg cgctttgtgt gtttcggctg aggcgtaata
    36721 taaaatattg cgagctcaat atattaaaag agtgccaggc ctgatgagcg tagcgcatca
    36781 ggcacatcgg ctaattacag attctcgccg ttgctggcaa tcacttcttt gtaccagtta
    36841 aagctcttct tacgtgaacg cgacatatcg ccagtaccgt cgtcatgttt attcacatag
    36901 ataaagccgt agcgtttgct gtactgcccg gtagtgaacg acacgcagtc gatgcagccc
    36961 cacggtgtgt agcccatcag atccacgcca tcgtaagtta ctgctttttt catctcttca
    37021 atatgggcgc gcaggtagtc aatgcggtag tcgtcgttga tgctgccatc ttcttccact
    37081 ttgtcgtaag cgccaaaacc gttttcgaca ataaacagcg gcttctggta acgctcatac
    37141 agttcgcaaa gtgcgtaacg cagacccacc ggatcaatct gccagcccca gtcagatgct
    37201 ttaacatacg ggtttggtac gctgccttca aaaccagaga tcgcatcgcc ggtgccgcct
    37261 tcggccttca ctgcgttggt catgtaatag ctgaaaccaa gataatcgca ggtgccttcg
    37321 cgcagtacat cgagatcgct atcttccatt ttgatgttaa atccgcgacg ctcccactcg
    37381 ttcaacacat acgacgggta atagccgcgt agctgcacat cggtaaagac gtagcgttcg
    37441 cgcatcgact cctgagcgaa catcacatcg tccgggttac atgagtaggg atagagcggc
    37501 accatcgcca gcatacagcc gactttcatc tccaggttaa tgcgacgcgc ggctttcact
    37561 gccagggcgc tggcgacaaa ctggtgatgc agcacctgat acatcgtctc ttccgggttt
    37621 tcatgctcgg tatacaccac gccggagcag cagtaaccga acagcggtgc acgccagtta
    37681 cgctggttgt taatttcgtt gaaggtcatc cagtatttga ctttgtgctt atagcgttca
    37741 aataccactt ccgcgaaacg tacaaagaaa tcaaccactt tacggttggt ccagctaccg
    37801 tattgctgca ccagatgcag cggcatttca aagtgggaga gggtgatcac cggttcgatg
    37861 ttgtatttca gcagttcatc gaacagagag tcgtagaact tcagcccttc ttcgtttggc
    37921 tgagcttcat cgccttttgg aaaaatgcgc gtccaggcaa tggatgtacg aaaacatttg
    37981 aagcccattt cggcaaatag cttgatgtct tccttatagt gaccataaaa atcaacggct
    38041 tcatggtttg gatagtattt gcctggcaag acttctttgg ttatttcgcg cggcacgccg
    38101 tgtgcgccac cggtcagaac gtcacaaatg ctcggccctt ttccgccttt gttccagccg
    38161 ccttcgacct gatgagcggc aactgcgccg ccccataaga aatctttcgg taaggtgagt
    38221 tttttcacta tcataggctc cagatgagtc aattcttaca ggaaatagta ccatgcagcc
    38281 aaacgacatc actttttttc aacgtttcca ggatgacatt ctggctgggc gtaaaaccat
    38341 caccatccgc gacgagtctg aatcgcactt caaaacgggt gatgtgcttc gtgtcggacg
    38401 ttttgaagat gacggttatt tttgcacgat tgaagtcacc gcaacctcaa ccgtaacgct
    38461 ggatacgctg acggaaaaac atgcagagca ggaaaatatg accctgactg aactgaaaaa
    38521 ggtcattgcc gacatctatc ccggccagac acagttttat gtgattgaat ttaaatgcct
    38581 ttaaattgaa ctcacgcctg ttagttgaaa tttcgcattt aatttcatga tttttaggag
    38641 attagtctca aatcagaatt tattttagct aacaggtgtt cactggaact attctcagtt
    38701 acgctggaga ggtatacatg tgcgtgtacg tttctccgga gtaagttatg gttcagaagc
    38761 ccctcattaa gcagggatat tcgctggcag aggaaattgc caacagcgtc agtcacggca
    38821 ttgggttggt gtttggtatc gttgggctgg tgttgctact ggttcaggcg gtggatctca
    38881 atgccagcgc cacagcgata accagctaca gcctctatgg cggcagtatg atcctgctgt
    38941 tcctcgcttc gacgctctat cacgccattc ctcatcaacg ggcaaaaatg tggctgaaga
    39001 aatttgacca ttgcgctatt tacctgttga ttgccggaac ctacacgccg tttttgctgg
    39061 tggggctgga ttctccgtta gcgcgcgggt tgatgattgt tatctggagc ctggcattgc
    39121 tggggattct gttcaaactg accatcgcgc accgattcaa aattttatct ctggtgactt
    39181 atctggcgat gggctggctg tcgctggtgg taatttatga aatggcagtt aagctcgcgg
    39241 cgggcagcgt taccttactg gcggtaggcg gcgtggttta ttcgctcggg gtgattttct
    39301 acgtctgcaa acgcattcca tacaaccatg ccatctggca cggcttcgtg ctcggcggta
    39361 gtgtgtgcca ctttctggcg atctatttgt atattgggca ggcgtaatgg gtatgccttt
    39421 gtaggcctga taagacgcgc cagcgtcgca tcaggcgata aatgcctgat gcgacacaga
    39481 gatttactct tcgagcgaat acggcagtgg ctcgatacac aatgtattcg catcgtcgcg
    39541 cacgcggaag atgctgtccg gttccatatc gttattcatc actacctgca ccacgacctg
    39601 accatcttcc agttttaccg cagccagcac cgtaccagta cgacgccagt tctcgcccat
    39661 tttcagctct aagtcttcac cagcttccgg cagtcggctg gcactacctg tcagcaacca
    39721 gagcgcgcgt ttgttggcac cacggaattt tgctcgcgcc accatctctt gtccggtata
    39781 gcagcctttc ttaaagctga taccgcccag cgcctggagg ttggtcgcct gtgggataaa
    39841 ctgcccgctg ttcgcggcat caatcaccgg gaaacccgct tcaatgttta atgccagcca
    39901 ctgttggcta ttgttcagtt ccgcttcacc gcgcagttta tcggtcagca tattggcagt
    39961 agcttcatcg gttacgatca ggaaacgttc tgccgggtgt tcaaaccata gcaaagtggt
    40021 cgcgccttct ttaactacct gtttttcttt cgaaggcagt tcactaaaga tatttgccag
    40081 cgcggcgcgc gcctgaaaac cggcaacacc aagcagcaca cgctcgtcgt ctggcgcgat
    40141 ggtcacttta gagaacaccg catatttttt cagttcagcc agctgcggtt cacgcacgct
    40201 gcgccgttca atccatgcaa agccatcgcc gtcgcggaac agacgtaaat tgctccacat
    40261 tttacctttg gcgtcgcaat gggcggcgag caggtgctga tcatctgtca tctggctgac
    40321 atctgctgtc acctggccct gcaaatattt ttcgctgtcc gcgccagtaa tggtggcaag
    40381 cgcccagtca tcaagcgtca tcagcgtcag tggcaaacgg gcagaagccg taggctgacg
    40441 gggaggaaaa ggtgtaaaag ccataataga gtcctgagtt gcttaacggc gtattaataa
    40501 ctaatggtaa aagagctatg aggcaatgca agtgctttca ctacgcgatc tgtgccttct
    40561 gcgaggcgac ttcccaaaaa atgaagccct taaattgttg tgaattaatt tcgcgatcgc
    40621 gattgtgttt actgatattc ccgtaaaaga agtcgaaaaa cacgttacaa tagccgggta
    40681 ctggattttc gtgagacagg aagaagatgg acattaacaa caaagcccgc attcattggg
    40741 catgccgccg tggtatgcgc gaactcgata tttcaatcat gccgtttttc gaacatgagt
    40801 atgacagctt aagcgatgac gaaaaacgca tctttattcg tctgctggaa tgtgacgatc
    40861 ccgatctgtt taactggctg atgaatcacg gtaaaccggc cgatgcagaa ctggaaatga
    40921 tggtccgact catccagaca cggaaccggg aacgtggtcc tgtggcaatc tgatttgcgc
    40981 gtctcctggc gcgcacagtg gctttccttg ctgattcatg ggctggttgc cgctgttatt
    41041 ttactcatgc cctggccgct cagttacacc ccgttatgga tggtgttact ttcgctggtg
    41101 gtgtttgatt gcgttcgcag ccagcggcgt attaatgctc gccaggggga aattcgcttg
    41161 ttgatggacg ggcgtttgcg ttggcaaggg caggagtgga gcatcgtcaa agcgccgtgg
    41221 atgattaaga gcggcatgat gctgcgttta cgttctgata gcggtaaacg gcaacattta
    41281 tggctggcag ccgacagcat ggacgaagcc gaatggcggg atttacggcg gattttgttg
    41341 caacaagaga cgcaaagata aagatgacgg caaagcatat gcgctaagcc gtcagtgata
    41401 tcaggcgtaa tgctctgcca tttcgttgag gatttgctcg caccagctct gaatacgctc
    41461 gtcgctaagg tcatactggt tagtttcgtc cagcgccagc cccacgaaca gttgcccgtc
    41521 agcaatcacc ggtttcgggc tggtaaattc atacccttcc gttggccagt agccgacgaa
    41581 cttcacgcct ttggtcgaga gtttgtcatg cagcataccg agcgcatcga ggaaccactc
    41641 gccgtatccc agttgatcgc caagcccata cagcgcaaca attttacctt cgaggttcag
    41701 gtcgtcgagc tgatcccaga cggcttccca gtcttcctgg atttcgccaa aatcccaggt
    41761 cgggataccc agaatgagca catcgtactg ctccattaat ttcggggagt cgtccttgag
    41821 gttatgtaag gtcaccagtt ctgggccgat aatatcgcgg attttttctg ccgccatttc
    41881 ggtgtaacag gtgctggaac cgtaaaaaag acccatattc ataacgtaag cttctcgatt
    41941 ctgctgtggc ttttgcggct agtgtatcag aatcgcttta gtcacggcac aataaggcat
    42001 aatggatcaa atgagtgacc ataaggggcg caagtgaaac aggatctggc acgcatcgag
    42061 cagtttcttg atgctctgtg gctggaaaaa aatctggctg aaaatacgtt gaacgcttac
    42121 cgtcgcgatc tgtcaatgat ggtggagtgg ttgcatcacc gcgggttgac gctggcgacg
    42181 gcgcaaagtg acgatttaca ggcattactg gcagaacggc tggagggcgg gtataaagcc
    42241 accagctcag cgcgtttgct gagtgcagtg cgccgattgt tccagtatct ttatcgcgaa
    42301 aagtttcgtg aagatgatcc cagtgcgcat ctcgcttcac cgaaattgcc ccagcgtttg
    42361 ccaaaagatt taagcgaagc gcaggtcgaa cgtttattac aggcaccatt aattgatcag
    42421 ccactggagc tacgcgataa agctatgctt gaagtgttgt atgctaccgg gctgcgtgtc
    42481 tctgaactgg tcgggctgac aatgagtgat atcagcctgc gtcagggcgt agtgcgggtc
    42541 attggtaaag gcaacaaaga gcgcctggtg ccgttaggtg aagaggcggt ttactggctg
    42601 gaaacctatc tggaacatgg gcgtccgtgg ctactgaatg gtgtgtcaat tgacgtgttg
    42661 tttcccagcc agcgtgcgca gcagatgacg cgacagacct tctggcaccg cattaaacat
    42721 tatgccgtgc tggcgggtat cgacagcgaa aagctgtcac cgcatgtgtt gcgtcacgct
    42781 tttgccactc acttattaaa tcatggtgcg gatttacgcg tggtgcagat gctactgggc
    42841 cacagcgatc tctccaccac gcaaatttat acgcatgtcg ctaccgagcg tctgcggcaa
    42901 cttcatcaac agcatcaccc gcgggcgtga tgtctgaaaa gaacgggaag atttatgaag
    42961 aaaggtttta tgttgtttac tttgttagcg gcgttttcag gcttcgctca ggctgatgac
    43021 gcggcaattc aacaaacatt agccaaaatg ggcatcaaaa gcagcgatat tcagcccgcg
    43081 cccgtagctg gcatgaagac tgtgctgact aacagcggtg tgttgtacat caccgatgat
    43141 ggtaaacata tcattcaggg gccaatgtat gacgttagcg gcacggctcc ggtcaatgtc
    43201 accaataaga tgctgttaaa gcagttgaat gcgcttgaaa aagagatgat cgtttataaa
    43261 gcaccgcagg aaaaacacgt catcaccgtg tttactgata ttacctgtgg ttactgccac
    43321 aaactgcatg aacaaatggc agactataac gcgctgggga tcaccgtgcg ttatcttgct
    43381 ttcccacgcc aggggctgga cagcgatgct gagaaagaaa tgaaagctat ctggtgtgcg
    43441 aaagataaaa acaaagcgtt tgatgatgtg atggcaggta aaagcgtcgc accagccagc
    43501 tgcgacgtgg atattgccga ccattacgca cttggcgtcc agcttggcgt tagcggtact
    43561 ccggcagttg tgctgagcaa tggcacactt gttccgggtt accagccgcc gaaagagatg
    43621 aaagaattcc tcgacgaaca ccaaaaaatg accagcggta aataattcgc gtgaaacaac
    43681 agatacaact tcgtcgccgt gaagtcgatg aaacggcaga cttgcccgcg gaattgcctc
    43741 ccttgctgcg ccgtttatac gccagccggg gagtacgcag tgcgcaagaa ctggaacgca
    43801 gtgttaaagg tatgctgccc tggcagcaac tgagcggcgt cgaaaaggcc gttgagatcc
    43861 tttacaacgc ttttcgcgaa ggaacgcgga ttattgtggt cggtgatttc gacgccgacg
    43921 gtgccaccag cacggctcta agcgtgctgg cgatgcgctc gcttggttgc agcaatatcg
    43981 actacctggt accaaaccgt ttcgaagacg gttacggctt aagcccggaa gtggtcgatc
    44041 aggcccatgc ccgtggcgcg cagttaattg tcacggtgga taacggtatt tcctcccatg
    44101 cgggagttga gcacgctcgc tcgttgggca tcccggttat tgttaccgat caccatttgc
    44161 cgggcgacac attacccgca gcggaagcga tcattaaccc taacttgcgc gactgtaatt
    44221 tcccgtcgaa atcactggca ggcgtgggtg tggcgtttta tctgatgctg gcgctgcgca
    44281 cctttttgcg cgatcagggc tggtttgatg agcgcggcat cgcaattcct aacctggcag
    44341 aactgctgga tctggtcgcg ctggggacag tggcggacgt cgtgccgctg gacgctaata
    44401 atcgcattct gacctggcag gggatgagtc gcatccgtgc cggaaagtgc cgtccaggga
    44461 ttaaagcgct gctggaagtg gcaaaccgtg atgcacaaaa actcgccgcc agcgatttag
    44521 gttttgcgct ggggccacgt ctcaatgctg ccgggcgact ggacgatatg tccgtcggtg
    44581 tggcgctgtt gctgtgcgac aacatcggcg aagcgcgcgt gctggcaaat gaactcgatg
    44641 cgctaaatca gacgcgaaaa gagatcgaac aaggaatgca gattgaagcc ttgaccctgt
    44701 gcgagaaact ggagcgaagt cgcgacacgc tacccggcgg gctggcaatg tatcaccccg
    44761 aatggcatca gggcgttgtc ggtattctgg cttcgcgcat caaagagcgt tttcaccgtc
    44821 cggttatcgc ctttgcgcca gcaggtgatg gtacgctgaa aggttccggt cgctccattc
    44881 aggggctgca tatgcgtgat gcgctggagc gattagacac actctacccc ggtatgatgc
    44941 tgaagtttgg cggtcatgcg atggcggcgg gtttgtcgct ggaagaggat aaattcgaac
    45001 tctttcaaca acggtttggc gagttggtta ccgagtggct ggacccttcg ctattgcaag
    45061 gcgaagtggt gtcagacggc ccgttaagcc cggccgaaat gaccatggaa gtggcgcagc
    45121 tgctgcgcga tgctggcccg tgggggcaga tgttcccgga gccgctgttt gatggtcatt
    45181 tccgtctgct gcaacagcgg ctggtgggcg aacgtcattt gaaagtcatg gtcgaaccgg
    45241 tcggcggcgg tccgctgctg gatggtattg cttttaatgt cgataccgcc ctctggccgg
    45301 ataacggcgt gcgcgaagtg caactggctt acaagctcga tatcaacgag tttcgcggca
    45361 accgcagcct gcaaattatc atcgacaata tctggccaat ttagcgtcat cttctctata
    45421 aaaaagagcg tggattgggt acaatcccgc tcttatcacc gcattttgac tagctcaata
    45481 aaagaaatca gaccatgttt gaaattaatc cggtaaataa tcgcattcag gacctcacgg
    45541 aacgctccga cgttcttagg gggtatcttt gactacgacg ccaagaaaga gcgtctggaa
    45601 gaagtaaacg ccgagctgga acagccggat gtctggaacg aacccgaacg cgcacaggcg
    45661 ctgggtaaag agcgttcctc cctcgaagcc gttgtcgaca ccctcgacca aatgaaacag
    45721 gggctggaag atgtttctgg tctgctggaa ctggctgtag aagctgacga cgaagaaacc
    45781 tttaacgaag ccgttgctga actcgacgcc ctggaagaaa aactggcgca gcttgagttc
    45841 cgccgtatgt tctctggcga atatgacagc gccgactgct acctcgatat tcaggcgggg
    45901 tctggcggta cggaagcaca ggactgggcg agcatgcttg agcgtatgta tttgcgctgg
    45961 gcagaatcgc gtggtttcaa aactgaaatc atcgaagagt cggaaggtga agtggcgggt
    46021 attaaatccg tgacgatcaa aatctccggc gattacgctt acggctggct gcgtacagaa
    46081 actggcgttc accgcctggt gcgtaagagc ccgtttgact ccggcggtcg tcgccacacg
    46141 tcgttcagct ccgcgtttgt ttatccggaa gttgatgatg atattgatat cgaaatcaac
    46201 ccggcggatc tgcgcattga cgtttatcgc gcgtccggcg cgggcggtca gcacgttaac
    46261 cgtaccgaat ctgcggtgcg tattacccac atcccgaccg ggatcgtgac ccagtgccag
    46321 aacgaccgtt cccagcacaa gaacaaagac caggccatga agcagatgaa agcgaagctt
    46381 tatgaactgg agatgcagaa gaaaaatgcc gagaaacagg cgatggaaga taacaaatcc
    46441 gacatcggct ggggcagcca gattcgttct tatgtccttg atgactcccg cattaaagat
    46501 ctgcgcaccg gggttgaaac ccgcaatacg caggccgtgc tggacggcag cctggatcaa
    46561 tttatcgaag caagtttgaa agcagggtta tgaggaacca acatgtctga acaacacgca
    46621 cagggcgctg acgcggtagt cgatcttaac aatgaactga aaacgcgtcg tgagaagctg
    46681 gcgaacctgc gtgagcaggg aattgccttc ccgaacgatt tccgtcgcga tcatacctct
    46741 gaccaattgc acgcagaatt cgacggtaaa gagaacgaag aacttgaagc gctgaacatc
    46801 gaagtcgccg ttgctggccg catgatgacc cgccgtatta tgggtaaagc ctcttttgtt
    46861 accctgcagg acgttggcgg tcgtattcag ctgtacgttg cccgtgacga tcttccggaa
    46921 ggcgtttaca acgagcagtt caaaaaatgg gacctcggcg acatcctcgg cgcgaaaggt
    46981 aaactgttca aaaccaaaac cggcgaactg tctatccact gtaccgagtt gcgtctgctg
    47041 accaaagcac tgcgtccgct gccggataaa ttccacggct tgcaggatca ggaagcgcgc
    47101 tatcgtcagc gttatctcga tctcatctcc aacgatgaat cccgcaacac ctttaaagtg
    47161 cgctcgcaga tcctctctgg tattcgccag ttcatggtga accgcggctt tatggaagtt
    47221 gaaacgccga tgatgcaggt gatccctggc ggtgccgctg cgcgtccgtt tatcacccac
    47281 cataacgcgc tggatctcga catgtacctg cgtatcgcgc cggaactgta cctcaagcgt
    47341 ctggtggttg gtggcttcga gcgtgtattc gaaatcaacc gtaacttccg taacgaaggt
    47401 atttccgtac gtcataaccc agagttcacc atgatggaac tctatatggc ttacgcagat
    47461 tacaaagatc tgatcgagct gaccgaatcg ctgttccgta ctctggcaca ggatattctc
    47521 ggtaagacgg aagtgaccta cggcgacgtg acgctggatt tcggtaaacc gttcgaaaaa
    47581 ctgaccatgc gtgaagcgat taagaaatat cgcccggaaa ccgacatggc ggatctggac
    47641 aacttcgact ctgcaaaagc gattgttgaa tctatcggta tccacgttga gaagagctgg
    47701 ggtctgggcc gtatcgttac cgagatcttc gaagaagtgg cagaagcaca tctgattcag
    47761 ccgaccttca ttactgaata tccggcagaa gtttctccgc tggcgcgtcg taacgacgtt
    47821 aacccggaaa tcacagaccg ctttgagttc ttcattggtg gtcgtgaaat cggtaacggc
    47881 tttagcgagc tgaatgatgc ggaagatcag gcgcaacgct tcctggatca ggttgccgcg
    47941 aaagatgcag gtgacgacga agcgatgttc tacgacgaag attacgtcac cgcactggaa
    48001 catggcttac cgccgacagc aggtctgggg attggtatcg accgtatggt aatgctgttc
    48061 accaacagcc ataccatccg cgacgttatt ctgttcccgg cgatgcgtcc ggtgaaataa
    48121 gcattacgtt atgcgcataa ccccggcaga tgtcggggtt ttttaattgt gctgcgcgaa
    48181 agcagacaac aattttcttg cttcactatt ggctgcctgc atcaccatcc acggactgaa
    48241 cgcccacggc gtggcatcaa taccgtgtaa tacatctgct aaatcacacc attgataatc
    48301 catcacttca tcatcgttga tctgtaacgc actggtggtg cgtgcggcaa ataccggaca
    48361 cacttcattt tccacaatgc cattcggatc ggtggcgcga tagcgaaagt caggatagat
    48421 agattcagga ggcgtaattt ccacgccaag ctcataacgg caacggcgga tcaccgcgtc
    48481 ttcgttgctt tctcccagtt gtgggtgccc acaaaccgag ttagtccaca cgccaggcca
    48541 tgcttttttg ctcagtgcgc ggcgggtaac taataattgt cctttggcat taaacagcca
    48601 actggagaac gcgagatgta agcgggtgtc tgccgtgtgt gcggcatact tttccagcgt
    48661 acccgtggga actccctgtg cattcaataa aatgacgtgt tccgtttgca taatttctca
    48721 catgtaattc tgatcactca tcattataga taattgaaga gtgaaatgat taaccaagcg
    48781 agcgtttatc gacaaccgcc cgttttgagc ggatgccgac aggtaaaaac catttaaaaa
    48841 agtagcatca tatatttgaa gcgactttca atggccattg caacacagtc agaaatgaca
    48901 ggaatagaga gagtaaaaag caacagcaga ctgaatatag cgcgcattaa aaaattcata
    48961 ttattctcca tgctcatttt tcttcagctt gcgcatcaga agccacatac gtactgtcct
    49021 gacttttaaa tctttatcag agacggattc cttcaccagg tcagcatgat gctgatagcc
    49081 attaaagaag acgttcagca caacggcgct aagggttgcg agcataatgc cgctatgtag
    49141 cagcggttgc agtacggccg gtaacttaga aaagaaatcg tgagagagcg ttggagtcat
    49201 gccaacgccg agactgatcg ccacaatata gaggttgtaa cggttggtgg tgtagttaca
    49261 gcgcgacaga attcgaatcc ctgtcgccag taccatgccg aacatcacca gaccagcgcc
    49321 gcccagcaca aattgcggaa tggaggctac cagcaccgcc atttttggca ccatgccaaa
    49381 caggattaat ataattcccg aagaaataca cacccagcgg ctatgaacgc gcgtcacgct
    49441 aaccaggcca acgttttgag aaaatgacgt gtgggggaag ctgttaaacg tgccaccaat
    49501 cattgtccct acgccatcga cacgcagccc gcgaataata tcgtgcgaag agagtttacg
    49561 accgactatt tcacccagtg ccaggaacat ccccatcgac tcgataaaca cgatgattaa
    49621 cacggcagtc atggtcagaa tggaaacggg atcgaaaatc ggcataccaa acgacatcgg
    49681 ggtgacaatc gcaaaccacg aggcatcatg tagcccggat aaattgactt cattcatcat
    49741 ccacgaaagt aaaaagccaa atacaatccc cagtaatacg gcgacgttgg acataaatcc
    49801 tttcgcatag cgagtaatga gcaagataaa aattaagacg gcaaaggaga tacctaaata
    49861 aacgggatta ccatattgcg gattcccttt acctccggcg gcccagtcaa tacccacctg
    49921 aatgatgcta agcccgatag aagtaataac cacaccggta accagtggcg ggaataaagg
    49981 catcaagcga ccgataagtt gcgctaataa tgtggtgata aaacccgcgg cgatagtggc
    50041 accaaatata cccagcaggc cgatatccgg gttcatccca atggctatca ttggtgttac
    50101 agcagcaaag gtcaccgaca taatcaccgg caggcggatc cccataaagc ggccgatacc
    50161 gatacattgc aataatgtga cgatcccgca gcaaaagaga tccgagctaa tgagcatcgc
    50221 aatagcttct tttgagaggc ccagtcggtc accaatcata agaggaacag cgactgcacc
    50281 tgcgtacatg acaaggacgt gttgcagacc gagtatgatc agctttcctg gtgataatat
    50341 gcggtcaacc tcatcagttg ggcggtcttg cccagaagat gagggaagtt gggaatctat
    50401 ggcgctcatc agtgtttctc ctttcgcccg ccagagtaaa aagcaaccgt ttacgttaat
    50461 gaaactgtct aaacacatgc tcgtggagtg ccagtttttg cgaggaaggg gagttagaga
    50521 caggaattgc ggaggcaatc acataatcgt gcttaaacta cgaaaatgac aagagaacct
    50581 gacaatggaa ttatctgtaa ttttctcata ttgatatttc attaatcatt ctggcatgtc
    50641 gcactctcgc atttaatcgt ttttatctgg atagcgctat ttggggcggc aatgttgaaa
    50701 taatgaaagc ggcatagatt gaggggcaat aatttgaact cagtcgcaaa attataatta
    50761 aaacggtcat gctacctaat atatgcccgt ttattgcatc gcgaaatttg agaagacata
    50821 actcattaaa tgaaagggat gcaaatgaat aagtttatcg ctgctgaagc cgcggaatgt
    50881 ataggctgtc atgcttgtga aattgcctgt gcggtggcac acaatcaaga aaactggccg
    50941 ctgagtcaca gtgactttcg accacgtatc cacgttgtag ggaaaggcct ggctgcgaat
    51001 ccggtggcct gccatcactg caacaatgcc ccttgcgtta cggcttgtcc ggttaatgct
    51061 ctgactttcc agtccgatag cgtacaactg gacgagcaaa aatgtattgg ttgtaaaaga
    51121 tgcgcaatcg cttgcccctt tggcgtcgtt gagatggtcg atacgattgc gcagaaatgc
    51181 gacctttgta accagcgcag ttccggcacg caagcctgta ttgaagtttg cccaacacag
    51241 gcgttacgac tgatggacga taaagggtta cagcagataa aggtggcccg ccagcgcaaa
    51301 acggcagcag gaaaagcgtc atcagacgct cagccatctc gcagtgcagc gttgctcccc
    51361 gttaactcgc gtaaaggcgc agataaaatt tcagcaagtg aacggaaaaa ccactttggc
    51421 gaaatctatt gcgggctgga tccacaacaa gcgacttatg agagtgaccg ctgtgtttat
    51481 tgtgccgaaa aagctaactg caactggcat tgcccgctgc ataacgctat tccggattac
    51541 atccgtctgg tacaggaggg aaagattatt gaagcggcag aactttgcca ccagaccagt
    51601 tccttaccag aaatctgcgg cagggtatgt ccacaggacc gtctttgtga aggcgcatgt
    51661 actttgaaag atcactctgg cgcagtctct atcggtaatc tggaacgcta catcaccgat
    51721 accgcgctgg cgatgggctg gcgtcccgat gtcagcaaag ttgttccccg tatcgagaaa
    51781 gtggcggtga ttggcgctgg gcctgcaggg ttagggtgtg ctgatattct ggcgcgcgca
    51841 ggagttcagg tcgatgtctt tgatcgccat ccagaaattg gcggtatgct gacttttggc
    51901 attcctcctt tcaaactcga taaaacggta ttaagccagc ggcgagagat attcaccgca
    51961 atgggaatcg actttcatct taactgtgaa attggccgcg atattacctt tagcgattta
    52021 acttctgaat atgatgcagt tttcatcggc gtggggactt acgggatgat gcgagcagat
    52081 ctgccgcatg aagatgcgcc cggtgtcatt caggctctac cgttcctgac tgcccatacc
    52141 cgccagctca tgggattgcc ggagtctgaa gagtatccgc tgacggacgt ggaaggtaag
    52201 cgagtcgtgg tattgggcgg tggcgatacg acaatggatt gtttgcggac ttccattcgc
    52261 ctcaatgctg ccagcgtgac ctgcgcgtat cgtcgtgatg aagtcagtat gccgggctcg
    52321 cgcaaagagg tggtcaatgc gcgcgaggaa ggtgtcgagt ttcaattcaa tgttcagccg
    52381 caatatatcg cttgtgacga agatgggcgt ttaactgcgg tgggcctgat tcgtaccgcc
    52441 atgggtgagc cggggccgga tggtcgccgt cgtcctcgtc cggttgcggg ttcagagttt
    52501 gaattgcccg ccgatgttct cattatggcc tttggtttcc aggcgcatgc catgccgtgg
    52561 ttgcagggca gcggaattaa actcgataaa tggggcctga ttcaaaccgg tgacgtcggg
    52621 tatttaccta cccagacgca tctgaaaaaa gtctttgctg gtggtgatgc agttcatggc
    52681 gcggatctgg ttgtcactgc aatggccgca ggaaggcagg cggcgcgcga tatgttaact
    52741 ctgtttgata cgaaggcatc gtgatgaaat cgttaattat cgttaatccg gctgactgta
    52801 ttggctgccg tacttgtgaa gtggcctgtg tggtcgctca cccttcataa caggagttga
    52861 atgccgatat ctttttaccc cggctgaagg tacagcgact ggactgcatc agtgcgccgg
    52921 tgatgtgcca tcagtgtgaa aacgcccctt gtgttggcgc ttgccccgtg ggggcgctga
    52981 cgatgggcga gcaggtggtg cagaccaatt ctgcccgttg tattggctgt cagagttgcg
    53041 tcagtgcgtg cccgtttgga atgatcacca ttcagtcgtt gccgggggat acccggcaac
    53101 aaatcgtgaa atgcgatctc tgtgaacagc gggaagaggg gcctgcctgc gttgaatctt
    53161 gcccaacaca ggcgctgcag ttgcttactg aaagagaact caggcgagtc cgccagcagc
    53221 gtattgttgc cagcggtgag aatcctctgt gaaagaaatc tccgcgctga aaggcgcgga
    53281 gaatctatta gattgcccaa ccacccgcgt aaaatgcgac cagtgcggca gtaatgatca
    53341 ccgtaccaat attcagcttt tgccattcgc gtgcaaagac gcggcctacg accagtgtca
    53401 caaagcccag cataataccg gtaacgatat tacaagtcag aacgatgaac acggcacaca
    53461 ccaggccagc catagcgtca ataaaatcat tgaaatccag cttcgagacg ttacttaaca
    53521 tcagcaaacc tacgtacatc agagcgggtg cagtggcgta accagggatc aaaaatgaca
    53581 gcggtgataa gaacagaatc agcaggaata acgcccccac tacggttgcg gttaaccctg
    53641 ttttaccccc ggcggcggtt cctgccgctg attcgatata aaccgctgcg ggcgctgcgc
    53701 ccaccaggcc ggagaatatt gaacttactg agtcactggt cagggctttg ccgccgttga
    53761 tgatctggtt gtctttatcc aacaaattcg cctgaccggc gacggcacgg atggtgccag
    53821 tagcgtcgaa cactgcggtc atcaccaatg ccagtacact cggaagtaca gttggctgga
    53881 gtgcgcccat aatatcgagg ctgaaaatca gagacttacc atcttcgcca gtcaggcttg
    53941 gcatcgccac cagaccgtgg tatttcaccg ctgggtcaaa gattaagccg atgatcgaaa
    54001 ttgcaataat caccaacaag ataccgccgg gtacgcgaca cttctccaga ccgaagatga
    54061 ccgccagccc cagcaagctc atcatcaccg ggaaggaggt aaacgcaccg agcgccactg
    54121 gcaagccttc aatcgggttt ttgataacca tacccacacc gttagcagca atcagcagca
    54181 gaaacagccc gataccgata cctgtaccgt gagcgatacc catcggcaaa ttacgtaaga
    54241 tccaggtacg cacaccggtt acggaaatgg cggtgaagat gacgcccatc agaaataccg
    54301 cgcccagtgc gacaggaacg ctaatttgtt gcccgagtac cagactgaat gcggtaaacg
    54361 ccgtcaagga aatcgcgcaa ccaatcgcca ttggcaaatt agcccataac cccatcagca
    54421 acgagccgaa gcccgcgacc agacaggtgg caacaaacac agctgcggga ggaaaacctg
    54481 ctttgcccag cattcccgga acgacgataa cggaataaac catggccaga aaggtcgtta
    54541 agccagccag tacttcctga cgaacggtac tgccacgagc ggtaatttta aaataattat
    54601 ccagcgcgcc ctgtggcttt ggtgcgtccg gtgtttgtag gatgtctcca gacataatga
    54661 tgtcctcacg aggatgtaaa agttttatta ttagttgcgt tcgtacacca gacgaccatc
    54721 aacgtaggtg cggtagatcg aacggtcatc gcccaacgtc atcatcacga acaatttgtc
    54781 gactaaagaa acagagttgt catagcgcag ctgctgtagc ggagtggcgg tgggttccat
    54841 caccacgaaa tcagcctctt tgccaggtaa aaagttgcca atcaaatcgt caaggcccag
    54901 agatttcgct ccgccgagcg tggccaggta aaacgcttca tatgccgaga ggcgatagcc
    54961 ttgtaattgc aacactttgt aggcttcgtt cagcgtttgc agcatgttga aagtggttcc
    55021 ggcaccgata tccgttccca tgcccacttt aactttcttc tgccatgctt ttttcaagtt
    55081 gaataacccg ctgccgaggt aaaggttgga ggtcggacag aaagcaatgc tggatttggt
    55141 ttcgctgaga cgatcccact ctttttcttt gagatggacg cagtgagcaa agacacagtt
    55201 tttaccggtc aggccgtact gatggtaaac atccagataa ccatcatggt caggataaag
    55261 cgatttcacc caggcaattt catctttgtt ttcacagaga tgggtatgta cccacgtatc
    55321 cggatattcc tctttcaggc gttgcgccat cgccatctgt tcaggagaag aagtaggggc
    55381 gaagcgtggc gtaatcgcat atagcagacg accatttttg tgccagcgtt caatcagttc
    55441 tttgctttgg tgatagctgc tttcggcagt gtctagcaga taatccggtg cgttgcggtc
    55501 catcatcacc ttaccggcaa tcatacgcat attgatatga ctggcggctt caaacagcgc
    55561 atcaacggat tgcggatgaa cagtgccaaa caccagcgcc gtggtggttc cgttacgtaa
    55621 aagctgcttg atgaagaacg ccgacatttc gcgggcgtac tctaaatcct cataacgacg
    55681 ttcagtaggg aaggtgtgtt tattcaacca ctccagcaat tgttccccat aggcccccac
    55741 catttcactt tgcggataat ggatatgtgt atcgacaaag cccggtacta tcagtttgcc
    55801 gcgatagtcg cgcacgcgaa tagtgtcagg aatttgatgc tttccgtctt cccattcgcc
    55861 aaaccattcc acttttccct gtttaatgag taataaacca tcctcaataa accgcagcgc
    55921 agaggcaatc tcttccgggt tatcgaccgt acgggcgaca tcaataaaac tgcctcgtac
    55981 cgcttttaac gtgtgttctc ctgacatcat tgactccttt actgttaatc catctcttcc
    56041 gctgaggtaa taccaggcag aacgttttcc tgtcggtagc caccagggag gataatattt
    56101 aataaaatcg cagttaaccc gccagcacaa atagggtttt caactaatac ataaatagag
    56161 gctggcaata ttttaaaaat ttcgggatcg taggagacgc caagtcctaa acctaaagaa
    56221 gtggcgacaa taagtgtttc tcggcgcttt aaaccgttgg tgatgatgat gcgaatccca
    56281 gcgatggcaa tcatggaaaa catcaacgtc attgcgcctc ccagaactgc cgagggaatg
    56341 gtcgtgaaga agccgccaat catcggaaac aaaccgagga taaccagcat taccgcgatg
    56401 gttcgcccga cataacgtga agcgacgcca gtcatctgaa taaccccatt attttgcgca
    56461 aacgtggtta agggtaatga cccgacagcg gaggcgataa cagaaaccag accgtctgcc
    56521 agcacgccgc ctttcagtcg ggactgatac tcttctccct gaatgggtcg gcgggaaacc
    56581 attgccgtgg cggtgatatc gccgacagct tccagcacgc taagcagata aatcgtgcca
    56641 accaccagga actgatggaa gctaaaacta aagccgtatt tgaacggatg tgggatggtg
    56701 attaacggca aattgcgcat actgctgaaa tccaccatgc ccaggcataa cgacgcgata
    56761 taaccgacgc atagcccaat ggcgatccct cccatgcgta gcaacggact gcgacagcag
    56821 ttaaagccga tcaccacaat caaaaccaat aaaccaacgc cgagatgttc gtaattgccg
    56881 aaggtaccgc tgcttttggc agcaaatcct ccaccaaaat cgataatgcc gactttaatc
    56941 aggcttaagc cgatcatcag taccacaata ccgctgacag taggcgtaat aacccgacgt
    57001 aaatagggca agataaatga agatccgaca accagaaatg cgccaacgaa ggagacgccg
    57061 aggagcgacg acatgattaa ctcttcgtga aaaccgtcgc ttttcatgct gctgcccagc
    57121 gcaatcatga ccgtaacaaa tgaaaaattg actgactgaa ttgagagtag gccagaaccg
    57181 acgatgccat agcggtttac ttgtaaccag gtaccaatac cagaggcgat catcgccatt
    57241 gaaacaagat aggcagttgt ttcagcggaa agctgtaagg ccgcccccac gattaacgcg
    57301 ggggttacca tcggaacgaa aattgccaac agatgggtaa tggcaccaac gagagcctga
    57361 tgaaagggag ggcgatcctc cagttcaaat ataaggtcag aacctgcatg gtttatatca
    57421 gacatccctt cactcctttt aaagtaaatt tgatcatctg aattagtaaa ataaattccc
    57481 acgaatttgg ggcgcaggac gccttgagaa cggcttatct cattttcgca gtcactattt
    57541 agtcatcgtc caaacagaga aacaggccgc cgaagcggcc atttattatt tatatctttt
    57601 ccagcgcggt gagtattttc tccggtgtga aatgccattc gcgtaaccag atgccgcatg
    57661 catcgtgaat tgcggtagca atcgccggag ctgcgccatt tacaccgatt tccgagatcg
    57721 atttcgcccc gaacgggccg actttatcgt cgctcggcac cagcactgcg cggaaatcac
    57781 gcggaatgtc accaattttc ggtgcgccgt agctgcgtaa atcacgcgtt aacgggtgac
    57841 cttcggcgtc ataaatgatc tcttcgctca tactgtggcc gatagctcgc agggtagcac
    57901 cgtagatttg tcccaacgct aactctggat tgaccggtgt accgcagtcc agcaaggcgt
    57961 agaatttatc caggcggatt tcacccgtac gcgtgttgac ggcaacttca gcgaagtttg
    58021 cgccatacgg gaaggcgaaa tcaggcgtga tataactgcc agttcccacc agtgaaccaa
    58081 agccggtgcc ggtttcgcct ttatgggcaa tatccccgaa actaacttcg cctttcttgc
    58141 cgcgcacgac gcccggcgtt gctagttgaa catctgccac tggctcaccc aacatttgcg
    58201 cgccgtggaa cagaattttc tcccgcagat tttccgctgc caaacgcgcc gcgttacccg
    58261 agaagcaagt accggacgag gcatatgcgc ctttatcaaa caacgcatga tcggtatcac
    58321 cggagataac atgcacgtcc tgcggtgggc agtgcagcac ttctgctgcc agtttcgtca
    58381 ccacggtatc cagaccagta ccaatatccg caccgccaga atgaacgata aaggtaccgt
    58441 ctgattccag tttgatcatg cagttagcct gatcgatatc cgggatccct gatttctgca
    58501 tgataatggc gacaccacga ccgatgtgcc agtcaccatt ttgtggtttt ggtgaagacc
    58561 attggatcat ctcgcgcccc tgacgcagga tctcttccag tgcgcagctg gcggcggaag
    58621 gaacggaggt cggcgcttta ccttcaccga ttgcaccgag aattttcagc tcttgccctt
    58681 cgtgtacccg gttacgttcg ataatttcca gttggtcgat ctgtaactgt tcagccagtt
    58741 ccgctaatgc catggtgata gcgaagttac ctttcggtgc gccataaccc tgataagcac
    58801 catttgggca aatgttgctg tagtaggtgg tgacctggaa atcgacgtta tcgcacggat
    58861 ataacggcag cgacagcgcc ggtccgttac acggtacggt gagtgagtgg ttgccgtaag
    58921 ggccagtgtt ggcgcggaaa tccatcttca ctgccgtcag gcgaccatct tttttcgctc
    58981 ccagtttgac ggtgactttc gcgacgtgac gagaggtgtt agcaataaac tcttcttcac
    59041 gggtgtagcg gaacagtacc ggacgcccgg tcacgcaggt tgcccaggcg cacacttctt
    59101 ccagcaggat gtcctgtttg gaaccaaaac cgccgccaac tcgctcttta atgacatgaa
    59161 ctttatgctg tttcatgtcc acgaggcgcg cgacctggcg gcgtaagtgc catggtacct
    59221 gggtggaggc gtggataacc agacgatcgc cgtccatccg ggtaaagcag atatgtgttt
    59281 cagtcgggca ctgctgcgcc tgcgttgagt tataggttcg ctcaatgatc acatcggcat
    59341 cggcaaagcc tttgtccata tcgccaatat gaccatgaat actagcggcg atatttttgc
    59401 gagggcgaga accgatcggg aagttgatga tcatatgctc gccgcgctgg gctgcatggc
    59461 tgttatcgtc ttccagagta tctggcgcac cagcaacata caccaccggt tcatcgtgca
    59521 cgacaggcgc atcttccgcc attgcttcgt cgatcgacat taccggctta agcacttcat
    59581 attcaacctc gatgagtttc aatgcttcga gcgcaatttc ttcactttca gcgaccaccg
    59641 cagcaacgcg atcgccgacg tgacgcattt ttttgccgaa catacggcgg tcaagcggtg
    59701 acggttccgg tgcgctctga ccccccgggg tatagtagat atcagggcaa ttcaggtgag
    59761 taataacgtg aacgacgccc ggtaaggctt cagctttgct gacatccaga tgagtaatca
    59821 gtgcgtgagc gtgtgggcta cgtaacattt taatgacgca ggcgtcagcc gttacgcggt
    59881 cttcaacata gcagggtttc gcctgcacca ttttcgcggc atcagtttta ggataatgct
    59941 tgccaatgac ttcaaggtcg tcacggaaag tcggagcgat atcaatggtg gcctgcggat
    60001 tatttttacg tgcaaccgcc agttcaatga cctgatagta ttgctgccag ccagcatcgc
    60061 ggctgaacaa accagaaagc gcgtcgtcga tctcttcgcg ggtaggtgcg gcgatgcgat
    60121 cgagaagatc ggtgataatc agagctgcag ctggatcgtt ataaccagac tgcaccacgc
    60181 caacatcaac cattgcctgt tgaaccagac ttaactcgtt ccatttgccc agagattctg
    60241 cggtacgaat atctgccttc tctaactgtg cggcaataag caaggacgcg ttaacgatat
    60301 taccgttaaa gatgattgcg tcagaaccgg caaacccgaa accatcatca ctgttgcgta
    60361 cagagtgcat tcccatgtta aacaacagct tttgcacgtt ttcgcctgga ttaacggtta
    60421 gctcctgagg cgcgccattt aaagtaaagt ggatgatcat acggcttcct ccccagcctg
    60481 ttggcagtcg gcatacaggt cggctaccag aactcccgtg atatagcgtt tataggccac
    60541 gctgccccgc aaatcttcct gcgggaaaat ggcgttggcg acagcctgtt caagtgcatt
    60601 gccttccaga ttttgttttt cgacatcatg cagacgcagt gctttactgg cgacgccatc
    60661 cagcgcaatt cgcataccgt cgtggtctgt cattgcaacg gctgccgtca cgacggttaa
    60721 acctgcctga gaacggctaa ttttgcgggt cgcacaggtg cgatacggat ctttaatgat
    60781 aatttcggtt agcaggcgat cgcatgggca ggccaggtag tcctcgattg acagcgtttc
    60841 gccgttgcca aaaaccagtt cagcatccag tgccagcagg acgggaagca gcactgactc
    60901 ttcctgccgg gcggcgattt caccaccaat ggtcgactga ttacgaacat ggcgtgagta
    60961 aacaaaaccg agggcttcac gcagtgctgc aggaataaat cgcgcatcac gcagtggctg
    61021 caagcgagac attgcgccaa tccgcagtgc accgttatcc cagtcaaccc agtccagttc
    61081 cagatcctgt aaggaaatgg caatcttttt atcggtacgg gttggtgtag cattgagttt
    61141 gctgcccccg gcgaaccaga cggcttcatc ctggtagcgg cgcttcagtt ccagcgcctg
    61201 ttcgacagag tcgggcctga aaaattgttc aatcatcttc tttccttatt taaccctctt
    61261 cctgcgggaa gagggcatct gtcatttatg ccagcgcatc catccgacgc cacatactgg
    61321 cagcggcttt tctggcttgt gcataaatgg aatcgcaatc gaagttaaac tgacggtctt
    61381 catagaccat cacaccattg accatcacgc tgtgaacgct gcctgagccc ataccgaaag
    61441 cgatatgccc ggcgatattg tctgccagca gcggcgtcgg cgagttgtaa tcgcaaatgg
    61501 ttaaatcagc tttgtaaccg gcttccagaa gcccaaattt cgcgccaaag ttgcggctca
    61561 tcagttcgtt accgttagtc agggctttgg caaaactgtc aggccacagc ggaccacccg
    61621 catcgcgatg tttaaagaag gcaaatttca tctcttcaaa catgtccgaa ccaatgccgt
    61681 ccgttcccaa cgccagattg cggatgtcgc taaggtgatg gttgtagccg acatggttgt
    61741 tcatgtttga acgggcgtta tgcaccagga acgcatcgcg ctgattgagt agggcgatgt
    61801 catctttcga caagtacagc ccatgagcga ccagcgtttt gctatcgatg agatcgaatt
    61861 gcgccagtcg tgccagcagg tctttgccgt accagtggtg actgtaggaa acgtcgtaaa
    61921 ggtcttccgc agcgtgaata tgcaaaccac ggcctgtggc gttcacggct tcacgtagca
    61981 tctccagacc ggcatctggc acggtaaacg gcgcgtgagc accgatatgt gcttccacca
    62041 gatacggctc ggttgctgct ttcttcgcct catcaatctg acgggcgaag cggatgtttt
    62101 cttctacacc ttcctgcaac tctttgatgc cgttgttacg gtcagtagtt tcaaaacagg
    62161 tcatcgcgcg caggccaact tttaaaaatg cgtcgcgcaa tgtggagagc gacccgccga
    62221 tatacgccgg agaggcatgg tgatcgataa ccgatgtaca tccgctctta atcgcttcca
    62281 gggaacaaat cagcccgctg taatagagcg actcttcatc aagggcgcga tcgagccgcc
    62341 accagagatt tttcagcgtt gagatgaaat ctgggctggg ggcgatgttt gccataattc
    62401 cgcgggaaag ccccgagtaa aaatggttgt gcgagcagac aattcccggc atcacaatcc
    62461 gaccatgcat ctctttgtag ctggcatcgg ggtagcgttg cgtcagggca tcgccgatag
    62521 cgacaatcac atcattttcg atagcgatat caacgccttc ctgcactttc gccgggtgta
    62581 actgcactgc ggtgacattc ttcagaatca acatgattac acctccacgc ggcccagcag
    62641 ataatgatga tgctggtgga catggctgat gatgcggcac atatcgttca gctccggtgg
    62701 tacgttgttg aactgacctt cgctgtcgat gtttaacacc cagctttggt tattcagtcg
    62761 tactcgtacc cggcaatctt ccacaaggaa gcctgggttg ctgctgttat caaagtcttg
    62821 cgccaggctg aaaacggtga ttttgtcttt gtatggttta ccgttccacg gacagaactg
    62881 agcgcagttg ccgcattcgt tacagtaagc gtcgaggtgc agcgtctgga atctattctg
    62941 gaagcctggg accgcaatgg agacgttggc gcggttcggg cagacatcca cacacttgct
    63001 gcaaacgtag ttacattcaa ggcagcgagc agcttcctgc gcgacaaacg catcacgatc
    63061 gtcactgttc accagcgtga tagagatatc gcctttacgt tgatagattt ccgccggatt
    63121 gacgttgttc cagtatttat cgttctggtg ggaacggata ttttcccgac taaggatggc
    63181 atcggtcgcc cgacgcgcgg ttccgacagc agcgacaatg gaggatggtc cgcgctgtac
    63241 gtcgccgatc ataaagacgt cagtcagacg agtttcgcca ttatggtcga cgtctggcca
    63301 gccgtttttg tccagcggca cgcccatcgc attcagggct tcagtatcct gctgttcacc
    63361 aatggcggtg atcaggctgt ctacatgcag tgttactgtt tcgttggttt caaccggacg
    63421 acgacgacct ttctcatcgg gttcgccaag cgacataacg cgcaaggtta aggtgccatc
    63481 agcatcgaaa cgttccggat tattcaggaa acggaactcc acgccgtcgt gcaacgcttc
    63541 ttcatactct tcgcgccatg cgggcatctc ttgcagtgaa cgacggtaaa cgaccgttgc
    63601 tttttctacg cctggaacgc gtaacgccgc acgagcgcag tccattgcgg tgttacccgc
    63661 cccgacaacg accacatgtt tgcccagctt gagcgctgta cccttgttgt attcacgcag
    63721 gaaggggagt gatttcaaga cattttggtt gtcgcccgcc agtttcacac cgctattttt
    63781 atctgtgccg gtggcaatca gaacatagtg gaagtcctga ttttttaact gctcaacggt
    63841 taaatcgggt gagcagccat actcaaattt cacgccgtga gcggcaacaa aatcgatatc
    63901 gtgctgaatt aactcagccg gaattcggaa ctgaggaatg atatttttca ccacgccgcc
    63961 cgcattggct tcgcgttcaa acagcgtaac cggatggccc gctctggcaa ggaagtaacc
    64021 tgctgccaga cccgccggac ctgcaccaat cacggcaacc ggatggcgtg aaccagaacc
    64081 ggctggtttg tgccagcgtt gcttatattc atcccaacct ttttccagcg cgactttttt
    64141 cagttcgcgg atattcagcg cactgtcgta atccaggcgg gtacagttgt attggcactg
    64201 gtgatcgcaa atatgaccgg taatggcggg cagagcgttg cgttggtaga tgagttccag
    64261 cgcgtcggca tagcggtgtt cgccaagcag acggatgtat tccggaatat cttgcttgat
    64321 agcgcaggca gtaacacagg gggcaacgta gcagtcggtc agcggcaggt cttctgccac
    64381 ttcaatacgc tcttctggct tccagtgttt ctgggtgtat tccatggtta acgcatctgc
    64441 tgccagtctg ttcagtcgtt cgacgtcaac atggtcaagt ccccaggcgt cggagccttc
    64501 cagctcgcgc atgcaggcac ttaagcgcaa atagccgcca ggtttcagca ggtcggttgc
    64561 catagtaata gggcgaatac ctgtatcaaa aatatcgcgg atagtcagct gactggcacc
    64621 accggaataa gaaatgggca gtttgccgtc aaaggcgcga gagagaactg ctgcaacatt
    64681 gatggagagc gggaacagcg cacggcctga catatacatc tcttcaccag gcagtgcgcc
    64741 tttattgttg atggtgccga gagtgttagt cagttttacg ccaaagccga gtgatttttc
    64801 ttttgccagt gccatcaggc gttccagcat ttccagtgct tgcgtcagct tgaggtcgtg
    64861 atcaaatgac tcttctttta agcctatgta accgaaaccg cagacatcga ggatctcacg
    64921 aacacgcgcg taccccagta aggtcgggtt aagtttcaca aaggtgttga gccctttttc
    64981 ttccagcatg tagcggcaaa tggcttcgat ttcatgcgga ggacagccgt gcatggtgga
    65041 gagggtgacg ccatgcacca tactggtggg gatgcgagcg ggtaaggctt gcaagctttc
    65101 gcgtttttcc tgcaatccgt ggcgggctaa aaatgcgtca tcctggagta atttattcag
    65161 cgtatcgcga tattgcgcga atttcggatg gtcagatgcg tccatcatat tgtcgatgaa
    65221 ctgttgcatt ggcggttgct taataccttc gaggttgtaa ccgacgctca tattaaagat
    65281 gaacgattta ccggaatcag aaggttggaa catcgcttcg agaaggtgca gggcaaacca
    65341 ggctttgagg tattcatccc aggctttaag cagggtaaat tcggtagacc attcggtgtt
    65401 aaagcactcg tcttcggcat caatacaggg cttttccagc tccaggcggt caagaatttg
    65461 gacggttttt agttcgatga aacgtccgcc agtcagccag gaagtgacaa tattttgcgc
    65521 gagctgcgtg tgcgggccag cggcagggcc gacgggagtg gcacaggttt caccgaagac
    65581 gctaacagtt ttacctttta caggtgagta aaactgttgc tcgggaatac caaagattga
    65641 gcgttgttgt tggtattcat caaatatgcg cgtcaaaagt tcctcaaacg gaatgggacg
    65701 cataatatcc cccataaccc tctccttagc ttatacagat atcattgagt gaccggtgtt
    65761 gagaaatccg gtggtttagg gggatagagc aacaatcgca ccactcttgc caggtttggc
    65821 gggaaatata aaaaaatgtg aggcggctcg aaacttaagg gctttttgtg aatgaactca
    65881 caggtgctaa atgagaatta aaatcactcc tgaaaattat tattgccatc ccatatatca
    65941 ctttttctct tttctcaaaa tcagtgattc cttatcatta tgataaagag tgctggaggg
    66001 attaaatatt tgaacgcaat cgtttaacta cgttttttaa att
//
LOCUS       DBFIFI010000006        65413 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.006, whole genome shotgun sequence.
ACCESSION   DBFIFI010000006 DBFIFI010000000
VERSION     DBFIFI010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65413
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.006"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..464)
                     /gene="folK"
                     /locus_tag="N7B39_000364"
     CDS             complement(<1..464)
                     /gene="folK"
                     /locus_tag="N7B39_000364"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HGU2420416.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILTVSSFYRTP
                     PLGPQDQPDYLNAAVALETTLAPEELLNHTQQIELQQGRVRKAERWGPRTLDLDIMLF
                     GNETINTERLTVPHYDMKNRGFMLWPLFEIAPELVFPDGETLREILTSLAVL"
     gene            complement(461..1858)
                     /gene="pcnB"
                     /locus_tag="N7B39_000365"
     CDS             complement(461..1858)
                     /gene="pcnB"
                     /locus_tag="N7B39_000365"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HGU2420417.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(1918..2814)
                     /gene="gluQRS"
                     /locus_tag="N7B39_000366"
     CDS             complement(1918..2814)
                     /gene="gluQRS"
                     /locus_tag="N7B39_000366"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HGU2420418.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARHGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHHAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVSEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWRLTAVPESAIVNSTFSNASC"
     gene            complement(2881..3336)
                     /gene="dksA"
                     /locus_tag="N7B39_000367"
     CDS             complement(2881..3336)
                     /gene="dksA"
                     /locus_tag="N7B39_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HGU2420419.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3514..4218)
                     /gene="sfsA"
                     /locus_tag="N7B39_000368"
     CDS             complement(3514..4218)
                     /gene="sfsA"
                     /locus_tag="N7B39_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HGU2420420.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4233..4763)
                     /gene="thpR"
                     /locus_tag="N7B39_000369"
     CDS             complement(4233..4763)
                     /gene="thpR"
                     /locus_tag="N7B39_000369"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HGU2420421.1"
                     /translation="MSDPQRLFFAIDLPAEIREQIIHWRATHFPLEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWVLTQ"
     gene            4837..7266
                     /gene="hrpB"
                     /locus_tag="N7B39_000370"
     CDS             4837..7266
                     /gene="hrpB"
                     /locus_tag="N7B39_000370"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HGU2420422.1"
                     /translation="MSSLPVAAVLPELLTALDYAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPTHQRFDDAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRISQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERATAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAGKWLPEYDWPAVDDESLLATLETWLLPHMAGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7462..9996
                     /gene="mrcB"
                     /locus_tag="N7B39_000371"
     CDS             7462..9996
                     /gene="mrcB"
                     /locus_tag="N7B39_000371"
                     /EC_number="2.4.99.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HGU2420423.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTDTWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10216..12459
                     /gene="fhuA"
                     /locus_tag="N7B39_000372"
     CDS             10216..12459
                     /gene="fhuA"
                     /locus_tag="N7B39_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HGU2420424.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLDLYNPVNTDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12510..13307
                     /gene="fhuC"
                     /locus_tag="N7B39_000373"
     CDS             12510..13307
                     /gene="fhuC"
                     /locus_tag="N7B39_000373"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HGU2420425.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13307..14197
                     /gene="fhuD"
                     /locus_tag="N7B39_000374"
     CDS             13307..14197
                     /gene="fhuD"
                     /locus_tag="N7B39_000374"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HGU2420426.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPESVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMNPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14194..16176
                     /gene="fhuB"
                     /locus_tag="N7B39_000375"
     CDS             14194..16176
                     /gene="fhuB"
                     /locus_tag="N7B39_000375"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HGU2420427.1"
                     /translation="MSKRIALFPALLLALLVIAATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGVALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16211..17491)
                     /gene="hemL"
                     /locus_tag="N7B39_000376"
     CDS             complement(16211..17491)
                     /gene="hemL"
                     /locus_tag="N7B39_000376"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HGU2420428.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPD
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17657..17704
                     /gene="yadX"
                     /locus_tag="N7B39_000377"
     CDS             17657..17704
                     /gene="yadX"
                     /locus_tag="N7B39_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HGU2420429.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17716..19137
                     /gene="clcA"
                     /locus_tag="N7B39_000378"
     CDS             17716..19137
                     /gene="clcA"
                     /locus_tag="N7B39_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HGU2420430.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDVFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19161..19226
                     /gene="yadW"
                     /locus_tag="N7B39_000379"
     CDS             19161..19226
                     /gene="yadW"
                     /locus_tag="N7B39_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HGU2420431.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19219..19563
                     /gene="erpA"
                     /locus_tag="N7B39_000380"
     CDS             19219..19563
                     /gene="erpA"
                     /locus_tag="N7B39_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HGU2420432.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19610..20233)
                     /gene="yadS"
                     /locus_tag="N7B39_000381"
     CDS             complement(19610..20233)
                     /gene="yadS"
                     /locus_tag="N7B39_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HGU2420433.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRHLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20271..21071)
                     /gene="btuF"
                     /locus_tag="N7B39_000382"
     CDS             complement(20271..21071)
                     /gene="btuF"
                     /locus_tag="N7B39_000382"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HGU2420434.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATNIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21064..21762)
                     /gene="mtnN"
                     /locus_tag="N7B39_000383"
     CDS             complement(21064..21762)
                     /gene="mtnN"
                     /locus_tag="N7B39_000383"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HGU2420435.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21846..23363
                     /gene="dgt"
                     /locus_tag="N7B39_000384"
     CDS             21846..23363
                     /gene="dgt"
                     /locus_tag="N7B39_000384"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HGU2420436.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAVL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRSPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23493..24917
                     /gene="degP"
                     /locus_tag="N7B39_000385"
     CDS             23493..24917
                     /gene="degP"
                     /locus_tag="N7B39_000385"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HGU2420437.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKLISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25072..26229
                     /gene="cdaR"
                     /locus_tag="N7B39_000386"
     CDS             25072..26229
                     /gene="cdaR"
                     /locus_tag="N7B39_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HGU2420438.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDVEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(26318..26704)
                     /gene="yaeH"
                     /locus_tag="N7B39_000387"
     CDS             complement(26318..26704)
                     /gene="yaeH"
                     /locus_tag="N7B39_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HGU2420439.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27019..27843)
                     /gene="dapD"
                     /locus_tag="N7B39_000388"
     CDS             complement(27019..27843)
                     /gene="dapD"
                     /locus_tag="N7B39_000388"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HGU2420440.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGDIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(27874..30546)
                     /gene="glnD"
                     /locus_tag="N7B39_000389"
     CDS             complement(27874..30546)
                     /gene="glnD"
                     /locus_tag="N7B39_000389"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HGU2420441.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVNVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEVLHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30608..31402)
                     /gene="map"
                     /locus_tag="N7B39_000390"
     CDS             complement(30608..31402)
                     /gene="map"
                     /locus_tag="N7B39_000390"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HGU2420442.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31770..32495
                     /gene="rpsB"
                     /locus_tag="N7B39_000391"
     CDS             31770..32495
                     /gene="rpsB"
                     /locus_tag="N7B39_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HGU2420443.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32753..33604
                     /gene="tsf"
                     /locus_tag="N7B39_000392"
     CDS             32753..33604
                     /gene="tsf"
                     /locus_tag="N7B39_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HGU2420444.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33751..34476
                     /gene="pyrH"
                     /locus_tag="N7B39_000393"
     CDS             33751..34476
                     /gene="pyrH"
                     /locus_tag="N7B39_000393"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HGU2420445.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34626..35183
                     /gene="frr"
                     /locus_tag="N7B39_000394"
     CDS             34626..35183
                     /gene="frr"
                     /locus_tag="N7B39_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HGU2420446.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35275..36471
                     /gene="ispC"
                     /locus_tag="N7B39_000395"
     CDS             35275..36471
                     /gene="ispC"
                     /locus_tag="N7B39_000395"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HGU2420447.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDASAREVARKE
                     VMRLAS"
     gene            36660..37418
                     /gene="ispU"
                     /locus_tag="N7B39_000396"
                     /gene_synonym="uppS"
     CDS             36660..37418
                     /gene="ispU"
                     /locus_tag="N7B39_000396"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HGU2420448.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37431..38288
                     /gene="cdsA"
                     /locus_tag="N7B39_000397"
     CDS             37431..38288
                     /gene="cdsA"
                     /locus_tag="N7B39_000397"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HGU2420449.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38300..39652
                     /gene="rseP"
                     /locus_tag="N7B39_000398"
     CDS             38300..39652
                     /gene="rseP"
                     /locus_tag="N7B39_000398"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HGU2420450.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39682..42114
                     /gene="bamA"
                     /locus_tag="N7B39_000399"
     CDS             39682..42114
                     /gene="bamA"
                     /locus_tag="N7B39_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HGU2420451.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42235..42720
                     /gene="skp"
                     /locus_tag="N7B39_000400"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42235..42720
                     /gene="skp"
                     /locus_tag="N7B39_000400"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HGU2420452.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            42724..43749
                     /gene="lpxD"
                     /locus_tag="N7B39_000401"
     CDS             42724..43749
                     /gene="lpxD"
                     /locus_tag="N7B39_000401"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HGU2420453.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            43854..44309
                     /gene="fabZ"
                     /locus_tag="N7B39_000402"
     CDS             43854..44309
                     /gene="fabZ"
                     /locus_tag="N7B39_000402"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HGU2420454.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44313..45101
                     /gene="lpxA"
                     /locus_tag="N7B39_000403"
     CDS             44313..45101
                     /gene="lpxA"
                     /locus_tag="N7B39_000403"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HGU2420455.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMAAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45101..46249
                     /gene="lpxB"
                     /locus_tag="N7B39_000404"
     CDS             45101..46249
                     /gene="lpxB"
                     /locus_tag="N7B39_000404"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HGU2420456.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NIILEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNSARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAAVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46246..46842
                     /gene="rnhB"
                     /locus_tag="N7B39_000405"
     CDS             46246..46842
                     /gene="rnhB"
                     /locus_tag="N7B39_000405"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HGU2420457.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALCEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            46879..50361
                     /gene="dnaE"
                     /locus_tag="N7B39_000406"
     CDS             46879..50361
                     /gene="dnaE"
                     /locus_tag="N7B39_000406"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HGU2420458.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQHLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50374..51333
                     /gene="accA"
                     /locus_tag="N7B39_000407"
     CDS             50374..51333
                     /gene="accA"
                     /locus_tag="N7B39_000407"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HGU2420459.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51432..53573
                     /gene="ldcC"
                     /locus_tag="N7B39_000408"
     CDS             51432..53573
                     /gene="ldcC"
                     /locus_tag="N7B39_000408"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HGU2420460.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALCF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53630..54019
                     /gene="yaeR"
                     /locus_tag="N7B39_000409"
     CDS             53630..54019
                     /gene="yaeR"
                     /locus_tag="N7B39_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGU2420461.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54084..55379
                     /gene="tilS"
                     /locus_tag="N7B39_000410"
     CDS             54084..55379
                     /gene="tilS"
                     /locus_tag="N7B39_000410"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HGU2420462.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGGAL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELAQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAETTARSAALCAE
                     QESLLDELLADDLAHCQSPQGTLQIVPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSENIVPWQTWLQPLELPA
                     GQGSVQLNAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGGFVTQEGVAEGENGISFVWQRNA"
     gene            complement(55432..55692)
                     /gene="rof"
                     /locus_tag="N7B39_000411"
     CDS             complement(55432..55692)
                     /gene="rof"
                     /locus_tag="N7B39_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HGU2420463.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55679..55879)
                     /gene="yaeP"
                     /locus_tag="N7B39_000412"
     CDS             complement(55679..55879)
                     /gene="yaeP"
                     /locus_tag="N7B39_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HGU2420464.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56045..56590
                     /gene="yaeQ"
                     /locus_tag="N7B39_000413"
     CDS             56045..56590
                     /gene="yaeQ"
                     /locus_tag="N7B39_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HGU2420465.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCAQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56587..56997
                     /gene="arfB"
                     /locus_tag="N7B39_000414"
     CDS             56587..56997
                     /gene="arfB"
                     /locus_tag="N7B39_000414"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HGU2420466.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRSGRE"
     gene            57011..57721
                     /gene="nlpE"
                     /locus_tag="N7B39_000415"
                     /gene_synonym="cutF"
     CDS             57011..57721
                     /gene="nlpE"
                     /locus_tag="N7B39_000415"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308221.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HGU2420467.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERGYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNQDCSSLGQ"
     gene            complement(57921..58745)
                     /gene="yaeF"
                     /locus_tag="N7B39_000416"
     CDS             complement(57921..58745)
                     /gene="yaeF"
                     /locus_tag="N7B39_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HGU2420468.1"
                     /translation="MDKPKAYCRLLLPCFLLLSACTVDISQPDSSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITVPDLKPGDLLFSSSLEVTSFGIRVFSTSSVSHVAIYLGENN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATEITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVVEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTR"
     gene            complement(58798..60516)
                     /gene="proS"
                     /locus_tag="N7B39_000417"
     CDS             complement(58798..60516)
                     /gene="proS"
                     /locus_tag="N7B39_000417"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HGU2420469.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIKK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFTAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60627..61334)
                     /gene="tsaA"
                     /locus_tag="N7B39_000418"
     CDS             complement(60627..61334)
                     /gene="tsaA"
                     /locus_tag="N7B39_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HGU2420470.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDCVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61331..61735)
                     /gene="rcsF"
                     /locus_tag="N7B39_000419"
     CDS             complement(61331..61735)
                     /gene="rcsF"
                     /locus_tag="N7B39_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HGU2420471.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQALCIGSALNITAK"
     gene            complement(61853..62668)
                     /gene="metQ"
                     /locus_tag="N7B39_000420"
     CDS             complement(61853..62668)
                     /gene="metQ"
                     /locus_tag="N7B39_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HGU2420472.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKNGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62708..63361)
                     /gene="metI"
                     /locus_tag="N7B39_000421"
     CDS             complement(62708..63361)
                     /gene="metI"
                     /locus_tag="N7B39_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HGU2420473.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVVGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63354..64385)
                     /gene="metN"
                     /locus_tag="N7B39_000422"
     CDS             complement(63354..64385)
                     /gene="metN"
                     /locus_tag="N7B39_000422"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HGU2420474.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64573..65148
                     /gene="gmhB"
                     /locus_tag="N7B39_000423"
     CDS             64573..65148
                     /gene="gmhB"
                     /locus_tag="N7B39_000423"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HGU2420475.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT        15415 a        16275 c        18063 g        15660 t
ORIGIN      
        1 tgtagtactg ctagcgaagt gagtatttca cgtaatgtct ccccatcagg aaacacgagt
       61 tccggggcaa tctcaaacag cggccacagc ataaagccgc gattcttcat atcgtaatgc
      121 gggacggtga gtcgttcggt attaatggtt tcattaccaa acaacatgat atcaagatcg
      181 agtgtgcgcg gtccccagcg ttctgcttta cgaacgcgac cttgctgcaa ctctatttgc
      241 tgtgtgtgat taagtaattc ttcaggcgca agtgttgttt ccagcgccac ggctgcgttt
      301 aagtaatcgg gttgatcttg cggccccagc ggtggggtgc ggtaaaacga agaaacggta
      361 agaatgtggc tttcagggat atcgcctaat gctttcaggg cagcattgac ctgctccagc
      421 ggagaggcca gattgctgcc tatggcaata tacgccactg tcatgcggta ccctcacgac
      481 gtggtgcgcg tttgcgtgga cgacgagtac gacgacgcgg tgacggttct tcatccagct
      541 cgttgagcat ccctttttgg tctggtggcg cggaaacctg gaactcaccc caccatttca
      601 ccagacgctg cagttcagcg ttacgctcaa cttcagctcg caaggccaac aggtcataag
      661 ccgcacggaa cttaggatgc tccagcaatt tccatgcgcg tttaccctga cgtcgggaca
      721 tacgcaactg caactgccag atatcgcggg ttaacgtcgt cagacgtttc gggattgcca
      781 gtgaacggca ggcttcgtcc agcacgtcgt tcatcgccag cgcgaaagcg tcgtgatagg
      841 tcaggccgct ttcctgggcg atcttctgtg ccgtctccag cagtgggtac cagaacatgg
      901 cggcaaacag gaacgccggg ttcacgcgca tatcgttatg gatacgcgta tcggtattct
      961 tcagcacctg ttcaatgatc cgctccatcg ggctgtcgcc attttccgtg aagtagcggg
     1021 taatggtcgg gaacagcggc tggaacagat gatattcaca caacagctta taggtttcgt
     1081 aaccgtagcc cgcttgtagc agtttaagcg attcttcaaa caggcgtgcc ggtgggatat
     1141 cgttcagcag ggtagcgagg cgagggatcg gttctgcggt ttccgggctg atgcgcatac
     1201 ccaatttggc ggcaaaacgt actgcgcgca gcatacgtac cggatcttca cggtagcgcg
     1261 tttccgggtt accaatcaga cggataacac cgtccttcag atccttcata ccgccaacgt
     1321 aatcacggac ggtaaaatcc gctacgctgt aatacaggct gttgatagtg aaatcgcggc
     1381 gctgggcgtc ttcttcgatg gagccgaaaa tgttgtcgcg cagcaacatg ccgttttgcc
     1441 cgcgttggga ggtcgtgcgg tcgctgacgt taccttcgtg gtgtccacgg aaggtcgcaa
     1501 cttcgataat ctccgggcca aacatcacat gagccagacg gaaacggcga cccaccaggc
     1561 ggcagttacg gaacagtttg cgcacctgct caggcgtggc gttagtggtt acgtcaaaat
     1621 ctttcggctt tttgccgagt aacaggtcgc gcacgccgcc gccaaccagc caggcttcgt
     1681 atcccgcttt attgagcctg tacattacct tcagggcatt ttcactgata tctttgcggg
     1741 aaatagcatg ctgctcacgc gggatcaccg tcacctgtgg acgggcgact gcctgttcag
     1801 cctcgctttc ctcgcggctt agcaccttgc ggcaaaaatt agcgactcgg gtaaaaatag
     1861 tacacctcgg tagtgtcaaa catcattcag gacaaaaaaa tagcggctaa tcatagctca
     1921 gcatgacgca tttgagaatg ttgaatttac aattgccgac tcaggcactg cggtaagccg
     1981 ccagtttttg acggctgact gaaggatttg ctcgacgctg aaatcctgcc agtgtgcttc
     2041 tgcctgctgc cccagaaatt gaagtgccgc gattagtacc gggcgtggat cgcctttcgg
     2101 caacgcaggc gcatgattct gcttggaaag tttagcgcct tgtggattaa gcgccagcgg
     2161 cagatgaatg taatctggca ctttccagcc aaaaagctgg tacagcgaga tttgccgcac
     2221 tgtcggttcg atcaaatctg ccccgcggac aatttcgctc acgccctgga agtgatcatc
     2281 caccaccacc gccaggttat aggcaaacaa accatcacgg cgatgaataa taaaatcttc
     2341 ccgtgccagt ttttcgtcgg cgtgaataat gccgcgcagc tggtcagtaa attgcgtgac
     2401 cggatgctgc tggcggatac gcactgcggc gttgtctggt ccatgatgca acacccggca
     2461 atgaccgtcg taaataccgc caatgctttg aatacgcgca cgcgtgcagg tgcagtaata
     2521 acttagtcct tgttcatgta accaggcgag tgcttcacga taggcgtggt gacgttgcga
     2581 ttgccagaga acatcgccgt cccagtgcag accgtaatgt tccagctggc gcaggatagt
     2641 ttctgcggca ccgggaactt cacgaggcgg gtcgatatct tcaatgcgta ccagccagcg
     2701 accgtgccgg gcgcgagcct gcaaatagct gccaagcgca gcaatcagag aaccaaaatg
     2761 aagctcgccg gaaggagagg gggcgaagcg gccaatatac tgtgtgtctg tcatctcttt
     2821 gaacaaaaaa taaggcggga gcatttcccg cctgtggtaa acgtgatgga acggctgtaa
     2881 ttagccagcc atctgttttt cgcgaatttc agccagcgtt ttgcagtcga tgcacagatc
     2941 ggctgtcggg cgcgcttcca gacggcgaat accaatttca acaccgcagg attcacagta
     3001 gccgaaatct tcgtcttcca cttttttcag cgtcttctcg atctttttga tcagcttacg
     3061 ctcgcgatcg cggttacgca gttcgaggct gaactcttct tcctgggctg cacggtctac
     3121 cgggtccggg aaattggctg cttcatcctg catatgtgta acggtgcgat cgacttcatc
     3181 cctgagttga ttacgccatg cttccagaat acgacggaag tgcgccagct gggcttcatt
     3241 catatactct tcgcccggct tctcctgata tggttccacc ccagcgatgg cgagaatact
     3301 cagggacgat gttttacggt tttgcccttc ttgcatgttg cttctcctta acacgcacta
     3361 tcgatcccca tgttcggggg aaaaatgagg ccgctataaa tagcagatgc ttttccggat
     3421 agcaattatc taaacgtaac acttgacaac tgtgtgagga aaagcgtatt tgcgcacgcg
     3481 accagaatgt aaattaacca gttacttact ttactacaat gtaaccggca gtgatttttt
     3541 aagagccatg ccttcagcag aaatttccgc tttgtaagcc agaatttcta cccccctctg
     3601 ttgagcttct gacaatagtt gcgcgtattt ctcatcgatg tggcgcgcgg gtgaaaaccg
     3661 tgtaatggct gaatgcagca cggcgaaaaa gataaccgca cgctggcctt cagccgctac
     3721 gctcatcaac tcccgaaggt gtttctgacc tcgttcagtg accgcatcgg gaaaatatcc
     3781 ctgttcgttc tccgctaacg taaccgattt cacttcaata tagcagtctg gacgcgaatc
     3841 cgcctgcaac ataaagtcaa tacggctgcg ttctgcgccg tattttactt cgcttttcag
     3901 cgagctatag cctgacagtt ctgaaattga ttcattaagg atagcctctt tcgtcaacct
     3961 gttagcccaa agcgtgttga cgcaaataaa tgcgccgctc tggctttgag ttaattccca
     4021 ggtgtgtggg tatttccgtt tggtgttgtc tgaagtcgaa taccagacgg tatcgccagg
     4081 cgttgcacaa ccggtcatcg cacccgtatt cgggcagtgt agcgttaatt cgcgaccatc
     4141 gggtgtgatc acatcggcta aaaaacgttt gtaacgctga attagcgtcg cgcgctgtag
     4201 agggggagaa aattccattg cgacaatcct tgttattgcg ttagcaccca gcgttttagc
     4261 ggcgtgtagc gtgtgcgtcc acgggcaaac gaggaggcgt aaagggtgaa ctccgtcacc
     4321 gcatatgacc agttaaaacc tggcggcggg attgtcaccg cctcgctggc gtcgcgcaat
     4381 aaggtaatat gtggatgaaa cggacgattg ctttgaaaac aaccgctgcg ggcagcctgt
     4441 gaacggagca tattcgccag ctggattaag ccgcgtggcg gctgacgcat ccctaaccac
     4501 accacacgcg aacgcagcca ttggccggcg tcatcaagcg tgagtgtgaa accaggttga
     4561 cgaatccgtc cggctaaaag agaaagcgcc ttctctttct ctgcgctcac ttcgcctaaa
     4621 aatgccagag tcagatgcaa attatcggcg gcgaccggac gtcccgcctc aagtgggaag
     4681 tgtgtggcgc gccagtggat aatctgttcg cggatttctg caggtaagtc gatagcaaag
     4741 aacagacgtt gcgggtcaga catgtgaggc actcggttat gaattaccgc gatgctacaa
     4801 tgtggcgcga agaatgttaa ccctctggag cgttttgtgt cgtcgttgcc cgttgctgcc
     4861 gtgttacctg aattacttac cgctctcgat tatgcgccgc aggtattatt aagtgcgccg
     4921 accggggccg ggaaatcaac ctggctgccg ctgcaactgc tggcgcatcc cggcattaac
     4981 gggaaaatta tcctcctgga gccacggcgt ctggcggcgc gtaacgtcgc gcaacggctg
     5041 gcggagctgc ttaacgaaaa gccaggcgat accgttggct accggatgcg tgcgcaaaac
     5101 tgcgtcgggc cgaatacccg cctggaagta gttaccgaag gcgtgctgac gcggatgatc
     5161 cagcgtgacc cggaactgag cggtgttgga ttggtgatcc ttgatgaatt tcatgagcgt
     5221 agcttgcagg cggatttggc gttggcgctg ttactcgatg tgcaacaagg tctgcgtgat
     5281 gaccttaaac tgctgattat gtcggctacg ctggacaacg accgcttgca gcaaatgctg
     5341 ccagaagcgc ccgtcgtcat ctcagaaggg cgctcgtttc cggttgaacg ccgttattta
     5401 ccgctgccca cgcatcagcg ttttgacgat gccgttgcgg tagccaccgc tgaaatgctg
     5461 cgtcaggaaa gcggatcatt actgttattt ttacctggcg tcggagaaat tcagcgtgtg
     5521 caggaacaac tggcttcgcg catcggcagt gatgtattgc tctgcccgct gtatggcgcg
     5581 ttgtcgctga acgatcagcg aaaagcgatc ctcccggcac cgcaagggat gcgcaaagtg
     5641 gtgctggcga ccaatattgc tgaaaccagt ttaaccattg aaggtattcg tctggtggtg
     5701 gattgtgccc aggaaagggt ggcgcgtttt gatccgcgaa cagggcttac gcggttgatt
     5761 actcagcgaa tcagtcaggc gtcgatgacg cagcgtgccg ggcgcgccgg gcgtctggag
     5821 ccgggtatct gcctgcattt aatcgccaaa gaacaagcag aacgcgctac cgcgcaaagt
     5881 gaaccggaga tcttacaaag cgatctttcc ggtttgctga tggaattact gcaatgggga
     5941 tgcagcgatc cggcgcagat gagctggctg gatcaaccgc caacggtgaa tctactggcc
     6001 gcgaaacgtc tgttacaaat gctgggggcg ctggacggtg aacggcttag tgcgcaaggg
     6061 caaaaaatgg cagcgctggg taacgatccg cgtttagcgg caatgctggt tagcgcaaag
     6121 agcgacgacg aagctgctac cgcggcaaaa attgccgcca ttctcgaaga gccgccacgg
     6181 atgggcaata gtgacctggg cgtggcgttt tcgcgcaatc aaccagcctg gcagcaacgt
     6241 agtcagcaac tgttaaaacg cttaaacgta cgcggcggtg aggcagacag ttcgcttatc
     6301 gcgccgctac ttgccggggc gtttgccgat cgcattgctc gccgccgtgg gcaagatgga
     6361 cgctatcaac tggcaaacgg catgggggcg atgcttgatg ccgacgacgc gctaagtcgc
     6421 cacgaatggt tgatcgcgcc gttattattg cagggcagcg cctcgccgga tgcgcggatt
     6481 ttactggcgt taccggtcga tattgatgaa ttagtacaac gctgtccgca gctggtgcag
     6541 caatctgaca ccgtggaatg ggatgacgcg caaggtacgc tgaaagcctg gcgtcggctg
     6601 caaatcggtc agttgacggt gaaagtgcag ccgttggcga aaccgtcaga agacgagttg
     6661 catcaggcga tgcttaacgg catccgtgat aaaggtttaa gcgtgctcaa ctggacggcg
     6721 gaagcggaac agctacgctt gcgtttgtta tgcgccggaa agtggttgcc agaatatgac
     6781 tggccagcgg ttgatgatga aagtttgttg gcgacgctgg aaacgtggct gctgccacat
     6841 atggccggtg tacattcact acgcggcctg aaatcactcg acatttatca ggcactacgc
     6901 ggattacttg attggggaat gcagcaacgt ctggatagtg aattgcctgc gcattacact
     6961 gtgccgacgg gaagccggat cgccattcgt tatcatgaag ataacccgcc cgcgctggcg
     7021 gtgagaatgc aggagatgtt tggtgaggcc actaatccga cgatcgccca ggggcgcgtg
     7081 ccgctggtgc tggaattgct ttcacccgcc caaaggccgc tgcaaatcac gcgtgatttg
     7141 ggggccttct ggaaaggggc gtatcgtgag gtgcaaaagg agatgaaagg gcgttatccc
     7201 aaacacgtct ggccggacga cccggcaaac acggcaccga cgcgacggac gaaaaagtat
     7261 tcgtaaggtt ggttttctcc ctctccctgt gggagagggc cggggtgagg gcaccagcgc
     7321 gcacgttcac cctcacccta accctctccc ttaagggaga ggggaccgat cgagctcaaa
     7381 ttttgagaga tatcttcttc tgtcttgtaa cagaagaaca gaaaatcggg cttttgcgcc
     7441 tgaatattgc ggagaaaaag catggccggg aatgaccgcg agccaattgg acgcaaaggg
     7501 aaaccgacgc gtccggtcaa acaaaaggta agccgtcgtc gttacgaaga tgacgatgat
     7561 tacgacgatt atgatgacta tgaggatgaa gaaccgatgc cgcgcaaagg taagggcaaa
     7621 ggcaaagggc gtaagcctcg tggcaaacgc ggctggctat ggctgctgtt aaaactggct
     7681 atcgtttttg ccgtgctgat cgccatttac ggcgtttatc tcgatcaaaa aattcgtagc
     7741 cgtattgatg gcaaggtctg gcaactgcct gcggcagttt atggtcgaat ggtcaacctt
     7801 gagccagaca tgaccatcag caagaacgag atggtgaagc tgctggaggc aacacagtat
     7861 cgtcaggtgt cgaaaatgac ccgtcctggc gaatttaccg tgcaggccaa cagcattgag
     7921 atgattcgcc gtccgtttga tttcccggac agtaaagaag ggcaggtgcg cgcgcgtctg
     7981 acctttgatg gcgatcatct ggcgaccatc gtcaatatgg agaacaaccg tcagttcggt
     8041 ttcttccgtc ttgatccgcg tctgatcacc atgatctcct caccaaacgg tgagcagcgt
     8101 ttgtttgtgc cgcgcagtgg tttcccggat ttgctggtgg atactttgct ggcgacagaa
     8161 gaccgccatt tctacgagca tgatggaatc agtctctact ccatcggacg tgcggtgctg
     8221 gcaaacctga ccgcaggtcg cacggtgcag ggggcgagta cgctgacgca acagctggtg
     8281 aaaaacctgt tcctctccag cgagcgctct tattggcgta aagcgaatga agcctacatg
     8341 gcgctgatca tggacgcgcg ttacagcaaa gaccgtattc ttgagctgta tatgaacgag
     8401 gtgtatctcg gtcagagcgg cgacaacgaa atccgcggct tcccgctggc gagcttgtat
     8461 tactttggtc gcccggtaga agaattgagc ctcgatcagc aggcgctgtt agtcggtatg
     8521 gtgaaaggag cgtccatcta caacccgtgg cgtaacccga aactggcgct ggagcgacgt
     8581 aatctggtac tgcgtctgct gcaacagcaa cagattattg atcaagaact ctatgacatg
     8641 ttgagtgcgc gtccgctggg ggttcagccg cgcggtgggg tgatctctcc tcagccagcc
     8701 tttatgcaac tggtgcgtca ggagcttcag gctaaattag gcgataaggt aaaagatctc
     8761 tccggcgtga agatcttcac tacctttgac tcggtggccc aggacgcggc agaaaaagcc
     8821 gccgtggaag gtattccggc actgaagaaa cagcgtaagt tgagcgatct tgaaactgcg
     8881 attgtggtcg tcgaccgctt tagtggtgaa gttcgtgcga tggtcggagg ttctgagccg
     8941 cagtttgcgg gctacaaccg tgcgatgcag gcgcgtcgtt cgattggttc ccttgcaaaa
     9001 ccagcgactt atctgacggc cttaagccag ccgaaaatct atcgtctgaa tacgtggatt
     9061 gcggatgcgc caattgcgct gcgtcagccg aatggccagg tctggtcacc gcagaatgat
     9121 gaccgtcgtt atagcgaaag cggcagagtg atgctggtgg atgcgttgac ccgttcgatg
     9181 aacgtgccga cggtaaatct ggggatggcg ctggggctgc ctgcggttac ggatacctgg
     9241 attaaactgg gcgtaccgaa agatcagttg catccggttc cggcaatgct gctgggggcg
     9301 ttgaacttaa cgccaatcga agtggcgcag gcattccaga ccatcgccag cggtggtaac
     9361 cgtgcaccgc tttctgcgct gcgttcggta atcgcggaag atggcaaagt gctgtatcag
     9421 agcttcccgc aggcggaacg cgctgttccg gcgcaggcgg cgtatctgac actatggacc
     9481 atgcagcagg tggtacaacg cggtacgggt cgtcagcttg gggcgaaata cccgaacctg
     9541 catctggcag ggaaaacagg gactaccaac aataacgtag atacctggtt tgcgggcatt
     9601 gacggcagca cggtgaccat cacctgggtc ggccgtgata acaaccagcc gaccaaactg
     9661 tatggagcca gcggggcaat gtcgatttat cagcgttatc tggctaacca gacgccaacg
     9721 ccgctgaatc ttgttccgcc agaagatatt gcagatatgg gcgtggacta cgacggcaac
     9781 tttgtttgca gcggtggcat gcgtgtcttg ccggtctgga ccagcgatcc gcaatcgctg
     9841 tgccagcaga gtgagatgca gcagcagccg tcaggcaatc cgtttgatca gtcttctcag
     9901 ccgcagcaac agccgcaaca gcaacctgct cagcaagagc agaaagacag cgacggtgta
     9961 gccggttgga ttaaggatat gtttggtagt aattaacatc taagcgtgaa ataccggatg
    10021 gcgagttgcc atccggtaaa ataacatccc acccaaagta ttaacccttt cttttcatct
    10081 ggttgtttat taacccttca ggaacgctca gattgcgtac cgcttgcgaa cccgccagag
    10141 tttcgaatat tatcttatct ttataataat cattctcgtt tacgttatca ttcactttac
    10201 atcagagata taccaatggc gcgttccaaa actgctcagc caaaacactc actgcgtaaa
    10261 atcgcagttg tagtagccac agcggttagc ggcatgtctg tttatgcaca ggcagcggtt
    10321 gaaccgaaag aagacactat caccgttacc gctgcacctg cgccgcaaga aagcgcatgg
    10381 gggcctgctg caactattgc ggcgcgacag tcagctaccg gcactaaaac cgatacgccg
    10441 attcaaaaag tgccacagtc tatttctgtt gtgaccgccg aagagatggc gctgcatcag
    10501 ccgaagtcgg taaaagaagc gcttagctac acgccgggtg tctctgttgg tacgcgtggc
    10561 gcatccaaca cctatgacca cctgatcatt cgcggttttg cggcagaagg ccaaagccag
    10621 aataactatc tgaatggcct gaagttgcag ggcaacttct ataacgatgc ggtcattgat
    10681 ccgtatatgc tggaacgcgc tgaaattatg cgtggcccgg tttccgtgct ttacggtaaa
    10741 agcagtcctg gcggcctgtt gaatatggtc agcaagcgtc cgaccaccga accgctgaaa
    10801 gaagttcagt ttaaagccgg tactgacagc ctgttccaga ctggttttga ctttagcgat
    10861 gcgctggatg atgacggcgt ttactcttat cgcctgaccg gtcttgcgcg ttctgccaat
    10921 gcccagcaga aagggtcaga agagcagcgt tatgctattg caccggcgtt cacctggcgt
    10981 ccggatgata aaaccaattt caccttcctt tcttacttcc agaacgagcc ggaaaccggt
    11041 tattacggct ggttgccgaa agagggaacc gttgagccgc tgccgaacgg taagcgtctg
    11101 ccgacagact ttaacgaagg ggcgaagaac aacacctatt ctcgtaatga gaagatggtg
    11161 ggctacagct tcgatcacga atttaacgac acctttactg tgcgtcagaa cctgcgcttt
    11221 gctgaaaaca aaacctcgca aaacagcgtt tatggttacg gcgtctgctc cgatccggcg
    11281 aatgcttaca gcaaacagtg tgcggcatta gcgccagcgg ataaaggcca ttatctggca
    11341 cgtaaatacg tcgttgatga tgagaagctg caaaacttct ccgttgatac ccagttgcag
    11401 agcaagtttg ccactggcga tatcgaccac accctgctga ccggtgtcga ctttatgcgt
    11461 atgcgtaatg acatcaacgc ctggtttggt tacgacgact ccgtaccgct gctcgatctg
    11521 tacaatccgg tgaataccga tttcgacttc aatgccaaag atccggcaaa ctccggccct
    11581 taccgcattc tgaataagca gaaacaaacg ggcgtttatg ttcaggatca ggcgcagtgg
    11641 gataaagtgc tggtcaccct gggcggtcgt tatgactggg cagatcaaga atctcttaac
    11701 cgcgttgccg ggacgaccga taaacgtgat gacaaacagt ttacctggcg tggtggtgtt
    11761 aactacctgt ttgataatgg cgtaacacct tactttagct atagcgaatc gtttgaacct
    11821 tcttcgcaag ttgggaagga tggtaatatt ttcgcaccgt ctaaaggtaa gcagtatgaa
    11881 gtcggcgtga aatatgtacc ggaagatcgt ccgattgtag ttactggtgc cgtgtataat
    11941 ctcactaaaa ccaacaacct gatggcggac cctgagggtt ccttcttctc ggttgaaggt
    12001 ggcgagatcc gcgcacgtgg cgtagaaatc gaagcgaaag cggcgctgtc ggcgagtgtt
    12061 aacgtagtcg gttcttatac ttacaccgat gcggaataca ccaccgatac tacctataaa
    12121 ggcaatacgc ctgcacaggt gccaaaacac atggcttcgc tgtgggctga ctataccttc
    12181 tttgacggtc cgctttcagg tctgacgctg ggcaccggtg gtcgttatac tggctccagc
    12241 tatggtgatc cggctaactc ctttaaagtg ggaagttata cggtcgtgga tgcgttagtg
    12301 cgttatgatc tggcgcgagt cggcatggcg ggctccaacg tggcgctgca tgttaacaac
    12361 ctgttcgatc gtgaatacgt cgccagctgc tttaacactt atggctgctt ctggggcgca
    12421 gaacgtcagg tcgttgcaac cgcaaccttc cgtttctaat ttctcttttg gggcacggat
    12481 ttccgtgcct gtttcacaag ttggctgtta tgcaggaata cacgaatcat tccgatacca
    12541 cttttgcact gcgtaatatc tcctttcgtg tgcccgggcg cacgcttttg catccgctgt
    12601 cgttaacctt tcctgccggg aaagtgaccg gtctgattgg tcacaacggt tctggtaaat
    12661 ccactctgct caaaatgctt ggccgtcatc agccgccgtc ggaaggggag attcttcttg
    12721 atgcccagcc gctggaaagc tggagcagca aagcgttcgc ccgcaaagtg gcttatttgc
    12781 cgcagcagct tcctccggca gaagggatga ccgtgcgtga actggtggcg attggtcgtt
    12841 acccgtggca tggcgcacta gggcgctttg gtgcggcaga tcgcgaaaaa gttgaggaag
    12901 ctatctcgct ggttggctta aaaccgctgg cgcatcggct ggtagacagc ctctctggcg
    12961 gcgaacgtca gcgggcgtgg atcgccatgc tggtggcgca ggatagccgt tgtctgttgc
    13021 tcgacgaacc gacttcggcg ttggatatcg cccaccaggt tgatgtgctg gcgctggtgc
    13081 accgtttaag tcaggagcgt ggcctgacgg tcattgccgt gctgcacgat atcaatatgg
    13141 ctgcgcgtta ctgtgattat ctggtcgccc tgcgcggcgg tgaaatgatt gctcagggaa
    13201 cgcctgcgga aattatgcgc ggcgaaaccc tcgaaatgat ttatggcatc ccgatgggta
    13261 ttctgccgca tccggcgggt gctgcacctg tgagttttgt ttattgatga gcggcttacc
    13321 tcttatttcg cgccgtcgac tgttaacggc gatggcgctt tctccgttgt tatggcagat
    13381 gaataccgcc cacgcggcgg ctattgaccc aaatcgtatt gtggcgctgg agtggctgcc
    13441 ggtggaatta ctactggcgc tcggcatcgt gccttatggc gtggcagaca ccatcaacta
    13501 tcgactgtgg gtcagcgaac caccactacc tgaatcggtg attgacgtcg gtttgcgtac
    13561 agaacctaac ctcgaactgc tgaccgaaat gaaaccgtcg tttatggtct ggtcggcggg
    13621 atatggtcct tcgccagaaa tgctggctcg tattgcgccg gggcgtggat ttaacttcag
    13681 cgacggcaaa cagccgctgg cgatggcgcg taaatcgctg acggaaatgg cggatttact
    13741 taacctgcaa agcgcagcag aaacgcattt agcgcaatat gaagacttta tccgcagcat
    13801 gaacccccgc tttgtgaagc gtggtgcgcg tccgttattg ctgacgacgc ttatcgatcc
    13861 gcgccatatg ctggtcttcg gtccaaacag cttgttccag gaaattcttg atgagtacgg
    13921 catcccaaat gcctggcaag gggaaaccaa cttctggggc agtaccgccg tcagcatcga
    13981 tcgtctggca gcgtataaag acgttgatgt gctctgtttt gatcacgaca acagcaaaga
    14041 catggatgcg ctaatggcaa cgccgctgtg gcaggccatg ccgtttgtcc gcgccggacg
    14101 ctttcagcgc gtacccgcag tctggtttta tggtgcaact ctctcggcaa tgcactttgt
    14161 gcgcgttctg gataacgcca ttggaggtaa agcgtgagta aacgtattgc gcttttcccg
    14221 gcattattgc tggcgttgct ggtgattgcc gctacggcgc tgacctggat gaacttctcg
    14281 caggcgctgc cgcgtagcca gtgggcgcag gctgcctggt cgccggatat tgacgtcatc
    14341 gagcagatga tttttcacta cagcttgctg ccgcgtctgg cgatttcgct gctggtgggc
    14401 gcaggcctgg ggctggtggg cgtgctgttt cagcaagtgc tgcgtaatcc gctggcggaa
    14461 ccgacgacac ttggcgttgc tacaggcgcg caactgggga ttaccgtcac tacgctctgg
    14521 gcgatcccag gggcgatggc gagtcagttt gctgcgctgg cgggcgcttg cgttgttggc
    14581 ttaatcgtct ttggcgtcgc gtgggggaaa cggctttcgc cggtaacgct gatcctcgcg
    14641 gggctggtag tgagccttta ttgtggggca atcaatcagt tactggttat cttccaccat
    14701 gaccaactgc aaagcatgtt cctgtggagc accggaacgc tgacgcaaac cgactggggc
    14761 ggtgttgagc gtttatggcc gcagttgctg ggcggtgtga tgctgacgtt gctgctactt
    14821 cgtccgttaa cactgatggg gcttgatgat ggcgtggcgc gcaatctcgg gctagccttg
    14881 tcgctggcgc gtctggcggc gctgtcgctg gctattgtca tcagtgcgct gctggtgaac
    14941 gcggtgggga ttatcggctt tatcgggttg ttcgcgccgt tgctggcaaa aatgctgggg
    15001 gcgcggcgtc tgctgccgcg actgatgctg gcgtcgttga ttggtgcgct gatcctctgg
    15061 ctttccgatc aaatcatcct ctggctgact cgcgtgtgga tggaagtgtc caccggttcg
    15121 gtcactgcgt tgatcggtgc gccgctgcta ctgtggctgt tgccgcgttt acgcagcatt
    15181 agcgcgccgg atatgaaggt caacgatcgt gtcgcggctg aacgccaaca tgtgctggcg
    15241 tttgccctcg cgggcggcgt gctgctgttg atggctgtgg tggtggcgct gtcgtttggt
    15301 cgtgatgcgc acggctggac gtgggcgagc ggggcgttgc tcgaggattt gatgccctgg
    15361 cgctggccgc gaattatggc ggcgctgttt gcgggcgtca tgctggcggt ggcgggctgt
    15421 attattcagc ggctgaccgg aaacccgatg gcaagcccgg aagtgctggg gattagctct
    15481 ggcgcggcgt ttggcgtggt actgatgctg tttctggtac cgggtaatgc ttttggctgg
    15541 ctgttacccg cgggaagtat cggtgcggcg gtgacgctgt tgattattat gatcgccgcc
    15601 gggcgcggtg gattttcccc acaccgcatg ttactggcgg ggatggcgtt aagtaccgca
    15661 ttcaccatgc ttttaatgat gttgcaggcg agtggtgacc cgcgaatggc gcaagtgctg
    15721 acctggatct ctggttcgac ctacaacgcc accgatgcgc aggtctggcg caccggaatt
    15781 gtgatggtga ttttgctggc gattaccccg ctgtgccgcc gctggctgac cattttaccg
    15841 ctgggtggtg ataccgcgcg ggccgtgggg gtggcgttaa cgccgacgcg aattgcgctg
    15901 ctgctgttag cggcttgcct gacggcgacc gcgacgatga ccattggacc gttgagtttt
    15961 gttggtttaa tggcaccgca tatcgcgcgg atgatgggct ttcgacggac gatgccgcac
    16021 atcgtgattt cggcgctggt gggtggttta ctgctggtgt tcgctgactg gtgtgggcgg
    16081 atggtgctgt ttccgttcca gatcccggcg gggctgctgt cgacctttat cggcgcgcca
    16141 tattttatct atttgttgag aaagcagagc cgttaataaa atgcctcgcc cggtgggcga
    16201 ggcaaaatca tcacaacttc gcaaacaccc gacgtgcagc atcgatggtg ttattgatat
    16261 cttccatgct gtgcgccaca gacataaagc ccgcttcaaa cgctgacggt gccaggtaaa
    16321 caccttcatc cagcatcata tggaagaaac gcttaaaacg ctcgacgtca caggccatca
    16381 catcctgata gcacgtcacg gactcggcgt cggtaaagaa aataccgaac atgccgccaa
    16441 cgtggtttac caccagcgga attccggctt cttctgccgc ttccagcaga ccttctgcca
    16501 gacgtgttgt cagctcatcc agcgtttcgt gaacgcccgg ctgcgcgact tcattcagac
    16561 aggcgaaacc ggctgccatc gcaatcgggt taccggaaag cgtacccgcc tgatagaccg
    16621 gacccgtcgg ggccagcgca tccattacat cacgacgacc accgaatgcg cctaccggca
    16681 ttccaccgcc gatgattttg cccaggcaag tgagatccgg ttccacaccg taataatcct
    16741 gtgcgccagc tagcgctacg cggaaaccgg tcattacttc atcgatgatc agcaacgcgc
    16801 caaattcgtc gcacagcgcg cgcagacctg gcaggaagtc tggcagcggt ggaacacagt
    16861 tcatattgcc tgccaccggc tcgacgataa tacaggcaat ctcttgcggg tattgctcaa
    16921 acgcggcgcg tacagaagcc agatcgttat aagtacaggt taaggtatgt ttggcgaaat
    16981 ctgccggaac gcccggcgag tttggctggc ctaacgtgag tgcgccagag ccggctttca
    17041 ccagcaggca gtcagcgtga ccgtggtaac aaccttcaaa tttaataatt ttgtcgcgac
    17101 cggtaaaacc acgggccagg cggatggcgc tcattgtcgc ttcagtgccg gagttcacca
    17161 tgcgcaccat atccatggtc gggaccagtt cggtcaccag ttgcgccatt ttcacttcca
    17221 tttcggttgg tgcaccaaag cttaaaccac gctcggcggc ttcaatcacg gcattgcgga
    17281 tcgccggatg gttatggccc agcaccatcg gcccccagga accgacataa tcgatatagg
    17341 ctttgccatc aacatcgtac agataagcgc cgtccgcttt ttcgataaac agtggagtgc
    17401 cgcccacgcc agtaaaggcg cgaacagggg agttcacacc gccagggatc agctcgcgcg
    17461 ctgcgctgta aagattttca gacttactca tggagggttc ctgattcgta gaaaaagtga
    17521 atggctgcta ttctatgtta ttcataacaa gttaaatact cgtcaaacat caggctgctt
    17581 gtactggtgc aatcctgatt tcttagagta taaaagtttt gtgcatttga aacattacgc
    17641 tttgcaaagg attttcatgg aacgtgcgag taaaatgccg tcatcttatt tgtatgacca
    17701 ataagtgatc attggatgaa aactgatact ccctctttag aaacaccgca ggccgcgcgc
    17761 ctgcgacgca gacaactgat tcgccaactt cttgagcgcg ataaaacccc gttagccatt
    17821 ttgtttatgg cggcagtcgt cggcacgctt gtcgggctgg cagcggttgc ttttgacaaa
    17881 ggtgtcgcct ggttgcagaa ccaacgtatg ggggcgctgg tacatactgc tgataattat
    17941 ccgctactgt taaccgttgc ttttctctgt tcggcggtgc tggcgatgtt cggctacttt
    18001 ttggtgcgca aatacgcgcc cgaagcaggg gggtcgggga tcccggaaat tgaaggggcg
    18061 ctggaagatc aacgtcctgt tcgctggtgg cgtgtattgc cggtgaaatt ctttggcggg
    18121 ctggggacac tcggcggagg catggtgttg gggcgcgaag ggccaaccgt gcagatcggc
    18181 ggtaatatcg ggcggatggt actcgatgtt ttccgcctga aaggtgatga agctcgccat
    18241 acgctgttgg caaccggtgc ggcggcgggt ctggcggcgg cctttaacgc gccgctggcg
    18301 ggtattttgt ttattatcga agagatgcgt ccgcagtttc gctatacgtt aatttcgatt
    18361 aaagcggtat ttattggcgt cattatgtcg accattatgt accggatttt taatcatgaa
    18421 gttgcgttga ttgacgtcgg taaactttct gacgcgccgc ttaatacgct gtggctttat
    18481 ctgatcctcg gtattatttt tggcattttc ggccctattt ttaataaatg ggtgctgggg
    18541 atgcaggatt tgctgcaccg tgtgcacggc ggcaatatta ccaaatgggt actgatgggt
    18601 ggtgcgattg gcggtctgtg tggattgctg gggtttgtgg caccagcaac gtcgggcggc
    18661 ggttttaacc tgattcctat cgctaccgcg gggaatttca gcatgggaat gctggtgttt
    18721 atcttcgtcg cgcgggtcat taccacctta ctctgcttct cttccggcgc gccgggcggt
    18781 atttttgccc cgatgctggc gctgggtact gtgctgggaa ccgctttcgg aatggttgcc
    18841 gttgagctgt ttccgcaata tcaccttgag gcggggacgt ttgctattgc cggaatgggg
    18901 gcgttactgg cggcatctat tcgcgcgccg ttaacgggga tcattctggt tctggagatg
    18961 accgataact accagctcat tttgccaatg attattaccg gtcttggcgc aacactatta
    19021 gcgcaattta ccggcgggaa accgctatac tcggcgattc ttgcgcgcac gctggcaaaa
    19081 caggaagctg agcaactggc gcgaagcaag gccgcatcag ccagcgagaa tacttgaacg
    19141 aaataccagg gtattagata atggcgatta ttattgggtt agaatttgcc caattgccga
    19201 tgtcgtttgg agcaaaatat gagtgatgac gtagcactgc cgctggagtt taccgacgca
    19261 gcagccaaca aagttaaaag cctgatcgct gacgaagata acccgaatct gaaattacgc
    19321 gtgtatatca ccggtggcgg ttgcagcggc ttccagtatg gtttcacctt tgatgatcag
    19381 gtgaacgaag gcgatatgac catcgaaaaa cagggcgttg gcctggtggt tgatccgatg
    19441 agcctgcaat atctggtcgg cggttccgtt gactataccg aaggactgga aggttctcgt
    19501 ttcatcgtga ccaacccgaa cgcgaaaagc acctgcggtt gcggttcttc ctttagtatc
    19561 taatctgttg ttcatgtgcc ggataaacta tccggcacat cttctctctt caacgcccat
    19621 tctcatccag cgcaaacgtc ggtagcttaa gatgccaacg aatagccgcc agccgaatca
    19681 atagcgtcac gaccatgccc atcatactgg ctgtttccag tggtacggaa aatgtgtaat
    19741 aagccgtagc gtggacaata ccgccgataa tacaggcagt tgcgtagatt tctgtacgta
    19801 aaatcatggg gatttcgcgg gccagaacat cacgaatgat cccgccgcca acgccagtaa
    19861 tgacgcccat acaaaccgcg attaacggac cggcttccgc attaaaggct ttattcacgc
    19921 caatgccgac aaacaccgcc agaccaacgg cgtccagcac cggcaacatc cattttggta
    19981 agtgtcttgg ctggcgcacc agcacgatgg tcagcatgct ggttaccatt gcaacgacca
    20041 gatcggtggg atctttcacc caaaataccg ggccgtgatc cagcgccatg tcgcgaattg
    20101 tcccgccgcc tacagcggta accacgccca gtaccagaac accaaaaggg tccatacgca
    20161 attttccggc taacaaaacg ccggagatgg caaataccgc tgtgccgact atatccagcc
    20221 aatagacgag cattgttcaa tccccactga gcacctaaaa aggtcaggcg ctaatctacc
    20281 tgtgaaagcg cattacagag ctgttgtgca gcgaggataa tacgtgggct tgcacgttca
    20341 aaccagtcac tcgtgagagg aataacggga attttgagct gttcacccca gtattgtttg
    20401 attttaggaa tttggtccgg tccgcctgta atgacaatcg cctgtggcga gcgtgctaac
    20461 acctgttcgc ggctaacttg cggccaggga acccggctgt ctttaaagat gttttctccg
    20521 ccacaaactt cgagtacctg gttttgaatc gactcttttc cactggtaaa tggcggatta
    20581 atgccgaatt gcagaaaaac acgtttttta ggtttatcag catattgcgc tttcaattgc
    20641 gcgtattgat ccagcaggga ttgcgcggct tgttcggcct tatccggttg cggactccag
    20701 ggagccagtt gacgtaacgc attggcaatt tgttcaatgt ttgtcgcatc gacccacatc
    20761 acttttattc ccagcgaagc cagctggtca acctgccgct cggcattacc tccacgccag
    20821 gcaatcacca gatcgggttt cagcgcgaca atgcgttcca gattcatccc ctgccaggtg
    20881 gaaacctgct caatcttttg cgcttgtgga ggatagtcgg aatagctgct gaccccaacc
    20941 ggcgtgatcc cggcggcaaa ggcaagttca gtgttggcgg gagaaagcgt gatgacgcgc
    21001 ggcgcggcgt tgagccacag tggcgcaaga aaagacaggg cgaccagcgc cctgaacagt
    21061 gacttagcca tgtgccagtt tctgcactag tgactcaacc atcaggctgg actgtttagc
    21121 ggcaacagcc aggaactcat cgaagctaag atgagactgt tgatcggcca cgtcggagat
    21181 ggcgcgtacg acaacaaacg ggacgttgaa attgtggcag acatgggcga ttgccgtcgc
    21241 ttccatctct acagcaatgg cctgtgggaa gttgtggcgg attttcgcca gaccaacaga
    21301 accgttgatg aaagcgtcgc cgctaacaat caggccacgt acagcgttaa gattcagttc
    21361 ggcaatgcag gcctcagcgg cagcgatcag tttatcgtca gctttaaagc ctgccggaca
    21421 gcctggtaac tgaccgtatt cataaccaaa tgccgtgaca tccgcgtcgt gataacgcgc
    21481 ttcgtccgag acaacgatat cgcccacttt cagcgttggt gccaggccac cggcagaacc
    21541 ggtgttaata atcacatctg gcttgcagtg ttccaacagc agagtggcac ccagcgccgc
    21601 agcgacttta ccgatgcccg atttcagaag cgcaacctcg gttccattca gttggccggt
    21661 atagatttcg caaccgccca gactgatagt ttgacggttt tcgattttgt cacgcagcag
    21721 cgtaacttct tcttccattg caccaatgat gccgattttc atagatttac tcgcgataag
    21781 cccgatttga aggcatagtt taccatgcgc ttacggggaa gcgtatttct cacgcgggag
    21841 aggacatggc acagattgat ttccgaaaaa aaataaactg gcatcgtcgt taccgttcac
    21901 cgcagggcgt taaaaccgaa catgagatcc tgcggatctt cgagagcgat cgcgggcgta
    21961 tcatcaactc tccggcaatt cgtcgtctgc aacaaaagac ccaggttttt ccactggagc
    22021 gcaatgccgc cgtgcgcacg cgtcttaccc actcgatgga agtccagcag gtggggcgct
    22081 acatcgccaa agaaatttta agccgtctga aagagcttaa attgctggaa gcatacggcc
    22141 tggatgaact gaccggacct tttgaaagca ttgttgagat gtcatgcctg atgcacgata
    22201 tcggcaatcc gccgtttggt cattttggcg aagcggcgat aaatgactgg tttcgccagc
    22261 gtttgcaccc ggaagatgcc gaaagccagc ctctgactga cgatcgctgc agcgtggcgg
    22321 tactacgttt acgggacggg gaagaaccgc ttaacgagct gcggcgcaag attcgtcagg
    22381 acttatgtca ttttgagggg aatgcacaag gcattcgtct ggtgcataca ttgatgcgga
    22441 tgaatctcac ctgggcgcag gttggcggta ttttaaaata tacccgtcca gcgtggtggc
    22501 gtggcgaaac gcctgagaca catcactatt taatgaaaaa gccgggttat tatctttctg
    22561 aagaagccta tattgcccgg ttgcgtaaag aacttaattt ggcgctttac agtcgttttc
    22621 cattaacgtg gattatggaa gctgccgacg acatctccta ttgtgtggca gatcttgaag
    22681 atgcggtaga gaaaagaata tttaccgttg agcagcttta tcatcatttg cacgaggcgt
    22741 ggggccagca tgagaaaggt tcgctctttt cgctggtggt tgaaaatgcc tgggaaaaat
    22801 cacgctcaaa tagtttaagc cgcagtacgg aagatcagtt ttttatgtat ttacgggtaa
    22861 acaccctaaa taaactggta ccctacgcgg cacaacgatt tattgataat ctgcctgcga
    22921 ttttcgccgg aacgtttaat catgcattat tggaagatgc cagcgaatgc agcgatcttc
    22981 ttaagctata taaaaatgtc gctgtaaaac atgtgtttag ccatccagat gtcgagcagc
    23041 ttgaattgca gggctatcgg gtcattagcg gattattaga gatttatcgc cctttattaa
    23101 gcctgtcatt atcagacttt actgaactgg tagaaaaaga acgggtgaaa cgttccccta
    23161 ttgaatctcg cttattccac aaactctcga cgcgccatcg gctggcctat gtcgaggctg
    23221 tcagtaaatt accgtcagat tctcctgagt ttccgctatg ggaatattat taccgttgcc
    23281 gcctgctgca ggattatatc agcggtatga ccgacctcta tgcctgggat gaataccgac
    23341 gtctgatggc cgtagaacaa taaccaggct tttgtaaaga cgaacaataa atttttacct
    23401 tttgcagaaa ctttagttcg gaacttcgcg ctataaaacg aatctgaaga acacagcaat
    23461 tttgcgttat ctgttaatcg agactgaaat acatgaaaaa aaccacatta gcactgagtg
    23521 cactggctct gagtttaggt ttggcgttat ctccgctctc tgcaacggcg gctgagactt
    23581 cttcagcaac gacagcccag cagatgccaa gccttgcacc gatgctcgaa aaggtgatgc
    23641 cttcagtggt cagcattaac gtagaaggta gcacaaccgt taatacgccg cgtatgccgc
    23701 gtaatttcca gcagttcttc ggtgatgatt ctccgttctg ccaggaaggt tctccgttcc
    23761 agagctctcc gttctgccag ggtggccagg gcggtaatgg tggcggccag caacagaaat
    23821 tcatggcgct gggttccggc gtcatcattg atgccgataa aggctatgtc gtcaccaaca
    23881 accacgttgt tgataacgcg acggtcatta aagttcaact gagcgatggc cgtaagttcg
    23941 acgcgaagat ggttggcaaa gatccgcgct ctgatatcgc gctgatccaa atccagaatc
    24001 cgaaaaacct gaccgcaatt aagatggcgg attctgatgc actgcgcgtg ggtgattaca
    24061 ccgtagcgat tggtaacccg tttggtctgg gcgagacggt aacttccggg attgtctctg
    24121 cgctggggcg tagcggcctg aatgccgaaa actacgaaaa cttcatccag accgatgcag
    24181 cgatcaaccg tggtaactcc ggtggtgcgc tggttaacct gaacggcgaa ctgatcggta
    24241 tcaacaccgc gatcctagca ccggacggcg gtaacatcgg tatcggtttt gctatcccga
    24301 gtaacatggt gaaaaacctg acctcgcaga tggtggaata cggccaggtg aaacgcggtg
    24361 agctgggtat tatggggact gagctgaact ccgaactggc gaaagcgatg aaagttgacg
    24421 cccagcgcgg tgctttcgta agccaggttc tgcctaattc ctccgctgca aaagcgggca
    24481 ttaaagcggg tgatgtgatc acctcactga acggtaagct gatcagcagc tttgccgcac
    24541 tgcgtgctca agtgggtact atgccggtgg gcagcaaact gaccctgggc ttgctgcgcg
    24601 acggtaagca ggtcaacgtg aatctggaac tgcagcagag cagccagaat caggttgatt
    24661 ccagctccat cttcaacggc attgaaggcg ctgagatgag caacaaaggc aaagatcagg
    24721 gcgtggtagt gaacaacgtg aaaacgggca ctccggctgc gcagatcggc ctgaagaaag
    24781 gtgatgtgat tattggcgcg aaccagcagg ctgtgaaaaa catcgctgaa ctgcgtaaag
    24841 ttctcgacag caaaccgtct gtgctggcac tgaacattca gcgcggcgac agcaccatct
    24901 acctgttaat gcagtaatct ccctcaaccc cttcctgaaa acgggaaggg ggtctcctta
    24961 caatctgtga acttcaccac aactccatac atcttcatca tcctttaggc atttgcacaa
    25021 tgccgtacgt tacgtacttc cttatgctaa gccgtgcata acggaggact tatggctggc
    25081 tggcatcttg ataccaaaat ggcgcaggat atcgtggcac gtaccatgcg catcatcgat
    25141 accaatatca acgtaatgga tgcccgtggg cgaattatcg gcagcggcga tcgtgagcgt
    25201 attggtgaat tgcacgaagg tgcattgctg gtactttcac agggacgagt cgtcgatatc
    25261 gatgacgcgg tggcacgtca tctgcacggt gtgcggcaag ggattaatct accgttacgg
    25321 ctggaaggtg aaattgtcgg cgtaattggc ctgacaggtg aaccagagaa tctgcgtaaa
    25381 tatggcgaac tggtctgcat gacggctgaa atgatgctgg aacagtcgcg gttgatgcac
    25441 ttgttggcgc aggatagccg tttgcgggag gaactggtga tgaacctgat tcaggcagag
    25501 gagaatactc ccgcacttac tgaatgggcg caacggctgg ggatcgatct caatcaaccg
    25561 cgagtggtgg ctattgttga ggtcgacagc ggtcagcttg gtgtggacag cgcaatggcg
    25621 gagttacaac aactgcaaaa cgcgctgact acgcccgagc gtaataatct ggtggcgatt
    25681 gtctcgctaa ccgaaatggt ggtgttgaaa ccggcgttga actcttttgg gcgctgggat
    25741 gtagaagatc atcgtaagcg agttgaacaa ctgattaccc gcatgaaaga gtacggccag
    25801 ctgcgttttc gcgtttcact gggcaactat tttaccggtc ctggcagtat tgcccgatcc
    25861 tatcgtacgg cgaaaacgac gatggtggtg ggtaaacagc ggatgccaga aagtcgctgc
    25921 tatttttatc aggatctgat gttacctgtg ttactcgaca gtttgcgtgg cgactggcag
    25981 gccaacgaac tggcgcgacc gctggcgcgg ctgaaagcga tggacaataa cggcttgctg
    26041 cgacgaacgc tggcggcgtg gtttcgtcac aatgtgcaac cgctggcaac gtcaaaggcg
    26101 ttgtttattc atcgtaatac cctggagtat cggcttaatc gtatatcgga actgaccggg
    26161 cttgatttgg gcaattttga tgacaggttg ctgctgtatg tggcgttaca actggatgaa
    26221 gagcggtagg ttatgcgtta aggttgaggc ggcgctggct cattcccctc agcccgaagg
    26281 ggagagggga atgcccgagt taaccgtcaa ttcgggatta tttacgcgtt agtttttcca
    26341 gatcggcttc aatctcgctg atcttattgg ttacgactga ctccaggtga cgtaagtcgt
    26401 cgaggatctt acgcttaaga tcaacttcgc tgcggtcacg ctggcagatt tgatcaagct
    26461 catcaatgat ataccgtaga ttcgggctga tttcctggac ttctttataa ccctgaccca
    26521 caccatcagc gacgaccgtt ttacgctgac gcggatattt aaacttaacg ctcttggcga
    26581 aaaactcgcc tttgtctttc tggaaataga ttttcagaat atcgttgttg gcttcctgcc
    26641 ggaggctgta acgatcaatt tcttcaggat tggtaatacc cagacttttc agattgtcgt
    26701 acatagcgtt acctcaaaat gagtcagtaa aatgtgctta ttttagcatt tggcctggcc
    26761 cgcccggctc gactgtgatc gcagaaagcc tttttcttcc agacttctat catggcgtaa
    26821 aaatcaaaaa attacctgct ttattgtggc gatattccat gggatggatt agcacattta
    26881 tcttcgtttt gcgaattaca tgacagtccg aaacaagatg aaaaagtatt taaaaaaggg
    26941 gggaggaaga atgagtagga cgaattattc tgaaccagaa agcaatcggg cagcgtcgtg
    27001 ctgcccgtgt gcatactttt agtcgatggt acgcagcagt tcgttaatgc cgactttgcc
    27061 gcgagttttc gcgtcaactt tcttaacgat aaccgcacag tagaggctgt atttgccatc
    27121 tttcgacggc agattacctg aaacaaccac agaccccgcc ggaacgcgac cgtagtggat
    27181 gtcgccggtt tcacggtcgt aaatacgggt gctctgacca atgtatacgc ccatggaaat
    27241 gacggaacct tcttcgacaa tcaccccttc aactacttca gagcgcgcgc cgatgaagca
    27301 attatcttca atgatggttg ggttagcctg cagcggttcc agcacaccgc cgatgcccac
    27361 gccaccggaa aggtggacgt ttttaccaat ctgcgcacaa gaaccgacgg tcgcccaggt
    27421 atcgaccatg gtgccttcat caacatatgc gccgatgttg acgtaagacg gcatcagcac
    27481 ggtgttacgg gcaataaacg caccctgacg taccgccgca ggtggtacaa cgcggaagcc
    27541 ttctttctgg aaacgtgctt cgtcgtagtc ggcgaatttc atcggcactt tgtcgaagta
    27601 gcggctttct gccccttcga tcacctgatt atcattaata cggaaagaga gcagcaccgc
    27661 ttttttcaac cactgatgcg tcacccactg accgtcaatt ttttccgcta cacgcagtgc
    27721 gccggaatcc agcagggcga tcacctgatt taccgcttcg cgggtaacgg tgtctgcatt
    27781 ggctggcgtg atctcggcac ggcgttcaaa agcggtttca ataatgttct gtaactgctg
    27841 cattgttaaa ctcttttcat atcagtaaac acatcaccct ttatcgtttg gattgagggc
    27901 ctctgtcaac cgctgatgca cttcctgctg caactcgtta ttaagtgcac gccggtcagc
    27961 ggtggcaatt atgaataaat cttctactcg ctcgccaatg gttgtaattc gggcaccatg
    28021 aagcgaaatt cccagatcgg caaaaatttt cccgactctc gccagcagtc ctggctggtc
    28081 gagcgcgata agctcaagga acgatttgcg gtcggtatgg gttggcaaaa acgtcacttc
    28141 ggtttcaaca gtaaagtgac gtagtttagc cggttgacga cgcggctgtg gtggctgcca
    28201 gctactttgt gtcagtactt gctccagacc aaaccgaata acctcatgac gatctgcgga
    28261 tagcgggcta ccatcgggtt ccagcacgat aaaggtatcc atcgccatac cgtcgcgagt
    28321 ggtgaaaatt tgtgcgtcgt gaacgcttaa gttgcgtcta tctaactcgg cacaaacagc
    28381 agcaaacaag taagggcggt ccgggctcca gataaaaatc tcggtgcctc cacgcgtagc
    28441 ctgcgggcta agcaatacca gcggtttgct taaatcatgc tgtaataaat ggcgcgcatg
    28501 ccaggccagt tgatttgggc tatgacggac aaaatagttc gcgcgacagc gtgaccaaat
    28561 ttggtgcagc acctcttcgt cgatgttatc catgcgcagt agtgccagcg cctggagttg
    28621 gtgatggcga acccgttcgc gcatatccgg cgtgttttgc attccgcgtc gtagctgctt
    28681 ttcggtggcg aaatagagtt cgcgcaacag actttgcttc cagctattcc acagcgtttc
    28741 gttggtggcg caaatgtcag ccactgtcag gcataccaga tagcgcagac gattttccgt
    28801 ttgaacttct tcggcaaact gcttgatgac ttccgggtcc tgaatatcgc gacgctgagc
    28861 tgtcaccgac atcagcaagt gctggcgaac cagccaggcg accagctgtg tttcgcgtga
    28921 gttcagcccg tggagttcgg caaaatgcac tacatcctga gcaccgagaa tagagtggtc
    28981 gccgccgcgt cctttggcga tatcgtgaaa cagcgcggcg atgaaaatca gctcagttga
    29041 cggtaggcgc ggccagacgt tcacacacaa cggatggcgc tggcgcgttt cttcactggc
    29101 aaaactctcc agtttcagca tcacgcggat agtatgttca tccaccgtgt aggcgtggaa
    29161 cagatcaaac tgcatctgcc cgacgatatg tgaccattgc ggcatatacg cgccgagtac
    29221 gctatggcga tgcattggca atagcccgcg ccgcaccgct ccggggtgac gtagaatgct
    29281 caaaaacagt tttcgtgctt gcggaatatt acacagcggt tgttgcagat ggcgacgggc
    29341 atggcgtaac tggcgcagtg tggtggagta aatgccggtg atcgcactgt tgcgcaccat
    29401 ggtgtagaac atacgcaaga tggcttccgg ctggcgcata aatagtgttt catcacgcag
    29461 gtcgattagc gtaccgcgta gctgaaactc atcgtcgatt ggacgtggtt tttcgtcggc
    29521 gggaagggcg aggatggctt catcgaacag ttgcagcagc atctggttga gttcactgac
    29581 gcggcgtgtc acgcggaagt aatccttcat catccgctcg accggttcgt taccttcgcc
    29641 actgtaattc agacgctggg cgacgctaag ctggcgatcg aataacaggc gattatcgta
    29701 acggctgacg accagatgca gggcaaagcg aatacgccac aatatatgca gacattcgtt
    29761 taattccgcc cgctccgctg aggttaagaa gccaaaccca accatttcat ccagcgatgt
    29821 tgcgccaaaa tgacggcggg ccacccattg cagagtgtgg atatcgcgca agccgccagg
    29881 gctacttttg atgtctggtt caagattgta gctggtgccg tggtaacgct gatggcgctg
    29941 gttctgttct tcaactttcg ccgcgtagaa cttgtcggaa ggccagaatc cttcgctgaa
    30001 aatatgtttt tgcagttcaa gaaatagcgc aacatcgcca attaataagc gggattcgat
    30061 caaattggtg gcgacggtta aatccgataa cccttccagc atgcactctt caagcgtgcg
    30121 cacactatga ccgacttcca gctttacatc ccagagcagc gttaacagct cgcccacttt
    30181 ttgcgcctga tcgtccggga gctttttacg gcttaaaatc agtaaatcga tgtcagaaag
    30241 tggatgcagc tcgccacgac cgtagccacc gacggcgacc aatgccaggt cggcaatctg
    30301 gctgaatccc gcttcaatcc ataatcgttg caggagctgg tcgataaact cggtgcgcgc
    30361 ctcaatcaac tgttctgcag agatcccatt gtcaaaggca tcacccagcc aacgctggaa
    30421 agtatcgata tgggctttta tcccaccgac ggttaattca tcacggggcc agacgcatgg
    30481 attttgcggt tgaccgggca gggtggggag agcggtgttt gcgtactgtt ctggaagggt
    30541 attcattgtg cgccacccat aagattaaat tatcgcatta aaaaagccgg cattcgccgg
    30601 cttcatttta ttcgtcgtgc gagattatcg ccgggatggt gtcatccttg cgtagcgtca
    30661 gaatttcgca gccgttatca gtcaccacaa tagtatgctc atattgtgca gacaagctgc
    30721 gatctttggt ttttaccgtc cagccatctt tcatggtgcg gatctctttt ttacccgcgt
    30781 tgaccattgg ctcgatggtg aacgtcatcc caggtttcag tacgacgttg gtttcacggg
    30841 agtcatagtg cagcacctgc ggttcttcat ggaagccgcg accaataccg tgtccgcaat
    30901 attcacgaac gacggagaag ccttctgctt cgacaaattt ctgaatcgcc gcaccgattt
    30961 cgcgcagatt aatgcctggt tttaccatgc gtagcgccag gtacaggctt tcttgcgtga
    31021 tacggcacag acgttcgccc atgatggtcg gcttaccgac gataaacatt ttcgaggtat
    31081 cgccgtggaa accatctttg attacggtga catcaatgtt aacgatatcg ccatctttca
    31141 gcagcttagc atcgtccgga ataccgtggc acaccacttc attaatagaa atgcaaacgg
    31201 atttcggata accgtgatag ccgaggcagg ctgaaaccgc gtgttgttca ttaacaatgt
    31261 aatcattaca gatgcgatcc agctcgccgg tgctgacgcc cggtttaaca tatggttcga
    31321 tcatctccag cacttcggca gccagtcggc cagcgacgcg cattttttcg atatcttctg
    31381 gggtcttgat tgagatagcc attaattctg tccatcagcg tcggtgatac cgacaatata
    31441 tatgtaagtg ccgtcaatgg tatcacaccc gggcaaattg agaatcattc tgaatttcgc
    31501 caaacgtgcc actgaaggtt ttctataata gaaaattcga cgtctgatgc tgtacacagc
    31561 gccaacaatt attggtgtcc acggcgtatt tgtggtataa agcgcgccgg acttccgatc
    31621 catttcgtat acacagactg gacggaagcg acaatctcac tttgtgtaac aacacacacg
    31681 tatcggcaca tattccgggg tgccctttgg ggtcggtaat atgggatacg tggaggcata
    31741 accccaactt ttatatagag gttttaatca tggcaactgt ttccatgcgc gacatgctca
    31801 aggctggtgt tcacttcggt caccagaccc gttactggaa cccgaaaatg aagccgttca
    31861 tcttcggtgc gcgtaacaaa gttcacatca tcaaccttga gaaaactgta ccgatgttca
    31921 acgaagctct ggctgaactg aacaagattg cttctcgcaa aggtaaaatc cttttcgttg
    31981 gtactaaacg cgctgcaagc gaagcggtga aagacgctgc tctgagctgc gaccagttct
    32041 tcgtgaacca tcgctggctg ggcggtatgc tgactaactg gaaaaccgtt cgtcagtcca
    32101 tcaaacgtct gaaagacctg gaaactcagt ctcaggacgg tactttcgac aagctgacca
    32161 agaaagaagc gctgatgcgc actcgtgagc tggagaaact ggaaaacagc ctgggcggta
    32221 tcaaagacat gggcggtctg ccggacgctc tgtttgtaat cgatgctgac cacgaacaca
    32281 ttgctatcaa agaagcaaac aacctgggta ttccggtatt tgctatcgtt gataccaact
    32341 ctgatccgga cggtgttgac ttcgttatcc cgggtaacga cgacgcaatc cgtgctgtga
    32401 ccctgtacct gggcgctgtt gctgcaaccg tacgtgaagg ccgttctcag gatctggctt
    32461 cccaggcgga agaaagcttc gtagaagctg agtaataagg cttgataact cccccaaaat
    32521 agttcgagtt gcagaaaggc ggcaagctcg agaattcccg ggagcttaca tcagtaagtg
    32581 accgggatga gcgagcgaag atgacgcatc tgcggcgcga aatatgaagg gggagagccc
    32641 ttatagacca ggtagtacac gtttggttag ggggcctgca tatggccccc tttttcactt
    32701 ttatatctgt gcggtttaat gccgggcaga tcacatctcc gaggatttta gaatggctga
    32761 aattaccgca tccctggtaa aagagctgcg tgagcgtact ggcgcaggca tgatggattg
    32821 caaaaaagca ctgactgaag ctaacggcga catcgagctg gcaatcgaaa acatgcgtaa
    32881 gtccggtgct attaaagcag cgaaaaaagc aggcaacgtt gctgctgacg gcgtgatcaa
    32941 aaccaaaatc gacggcaact acggcatcat tctggaagtt aactgccaga ctgacttcgt
    33001 tgcaaaagac gctggtttcc aggcgttcgc agacaaagtt ctggacgcag ctgttgctgg
    33061 caaaatcact gacgttgaag ttctgaaagc acagttcgaa gaagaacgtg ttgcgctggt
    33121 agcgaaaatt ggtgaaaaca tcaacattcg ccgcgttgct gcgctggaag gcgacgttct
    33181 gggttcttat cagcatggtg cgcgtatcgg cgttctggtt gctgctaaag gcgctgacga
    33241 agagctggtt aaacacatcg ctatgcacgt tgctgcaagc aagccagaat tcatcaaacc
    33301 ggaagacgta tccgctgaag tggtagaaaa agaataccag gtacagctgg atatcgcgat
    33361 gcagtctggt aagccgaaag aaatcgcaga gaaaatggtt gaaggccgca tgaagaaatt
    33421 caccggcgaa gtttctctga ccggtcagcc gttcgttatg gaaccaagca aaactgttgg
    33481 tcagctgctg aaagagcata acgctgaagt gactggcttc atccgcttcg aagtgggtga
    33541 aggcatcgag aaagttgaga ctgactttgc agcagaagtt gctgcgatgt ccaagcagtc
    33601 ttaattatca aaaaggagcc gcctgagggc ggcttctttt tgtgcccatc ttgtaaattc
    33661 agctaaccct tgtggggctg cgctgaaaag cgacgtacaa tgtcgctagt attaattcat
    33721 ttcaatcgtt gacagtctca ggaaagaaac atggctacca atgcaaaacc cgtctataaa
    33781 cgcattctgc ttaagttgag tggcgaagct ctgcagggca ctgaaggctt cggtattgat
    33841 gcaagcatac tggatcgtat ggctcaggaa atcaaagaac tggttgaact gggtattcag
    33901 gttggtgtgg tgattggtgg gggtaacctg ttccgtggcg ctggtctggc gaaagcgggt
    33961 atgaaccgcg ttgtgggcga ccacatgggg atgctggcga ccgtaatgaa cggcctggca
    34021 atgcgtgatg cactgcaccg cgcctatgtg aacgctcgtc tgatgtccgc tattccattg
    34081 aatggcgtgt gcgacagcta cagctgggca gaagctatca gcctgttgcg caacaaccgt
    34141 gtggtgatcc tctccgccgg tacaggtaac ccgttcttta ccaccgactc agcagcttgc
    34201 ctgcgtggta tcgaaattga agccgatgtg gtgctgaaag caaccaaagt tgacggcgtg
    34261 tttaccgctg atccggcgaa agatccaacc gcaaccatgt acgagcaact gacttacagc
    34321 gaagtgctgg aaaaagagct gaaagtcatg gacctggcgg ccttcacgct ggctcgtgac
    34381 cataaattac cgattcgtgt tttcaacatg aacaaaccgg gtgcgctgcg ccgtgtggta
    34441 atgggtgaaa aagaagggac tttaatcacg gaataattcc cgtgatggat aaataagggt
    34501 aagattccgc gtaagtatcg cgggggatct tacccgtaat atgtttaatc agggctatac
    34561 ttagcacact tccactgtgt gtgactgtct ggtctgactg agacaagttt tcaaggattc
    34621 gtaacgtgat tagcgatatc agaaaagatg ctgaagtacg catggacaaa tgcgtagaag
    34681 cgttcaaaac ccaaatcagc aaaatacgca cgggtcgtgc ttctcccagc ctgctggatg
    34741 gcattgtcgt ggaatattac ggcacgccga cgccgctgcg tcagctggca agcgtaacgg
    34801 tagaagattc ccgtactctg aaaatcaacg tgtttgatcg ttcaatgtct ccggccgttg
    34861 aaaaagcgat tatggcgtcc gatcttggcc tgaacccgaa ctctgcgggt agcgacatcc
    34921 gtgttccgct gccgccgctg acggaagaac gtcgtaaaga tctgaccaaa atcgttcgtg
    34981 gtgaagcaga acaagcgcgt gttgcagtac gtaacgtgcg tcgtgacgcg aacgacaaag
    35041 tgaaagcact gttgaaagat aaagagatca gcgaagacga cgatcgccgt tctcaggacg
    35101 atgtacagaa actgactgat gctgcaatca agaaaattga agcggcgctg gcagacaaag
    35161 aagcagaact gatgcagttc tgatttcttg aacgacaaaa acgccgctca gtagatcctt
    35221 gcggatcggc tggcggcgtt ttgcttttta ttctgtctca actctggatg tttcatgaag
    35281 caactcacca ttctgggctc gaccggctcg attggttgca gcacgctgga cgtggtgcgc
    35341 cataatcccg aacacttccg cgtagttgcg ctggtggcag gcaaaaatgt cactcgcatg
    35401 gtagaacagt gcctggaatt ctctccccgc tatgccgtaa tggacgatga agcgagtgcg
    35461 aaacttctta aaacgatgct acagcaacag ggtagccgca ccgaagtctt aagtgggcaa
    35521 caagccgctt gcgatatggc agcgcttgag gatgttgatc aggtgatggc agccattgtt
    35581 ggcgctgctg ggctgttacc tacgcttgct gcgatccgcg cgggtaaaac cattttgctg
    35641 gccaataaag aatcactggt tacctgcgga cgtctgttta tggacgccgt aaagcagagc
    35701 aaagcgcaat tgttaccggt cgatagcgaa cataacgcca tttttcagag tttaccgcaa
    35761 cctatccagc ataatctggg atacgctgac cttgagcaaa atggcgtggt gtccatttta
    35821 cttaccgggt ctggtggccc tttccgtgag acgccattgc gcgatttggc aacaatgacg
    35881 cccgatcaag cctgccgtca tccgaactgg tcgatgggac gtaaaatttc tgtcgattcg
    35941 gctaccatga tgaacaaagg tctggaatac attgaagcgc gttggctgtt taacgccagc
    36001 gccagccaga tggaagtgct gattcacccg cagtcagtga ttcactcaat ggtgcgctat
    36061 caggacggca gtgttctggc gcagctgggg gaaccggata tgcgtacgcc aattgcccac
    36121 accatggcat ggccgaatcg cgtgaactct ggcgtgaagc cgctcgattt ttgcaaacta
    36181 agtgcgttga catttgccgc accggattat gatcgttatc catgcctgaa actggcgatg
    36241 gaggcgttcg aacaaggcca ggcagcgacg acagcattga atgccgcaaa cgaaatcacc
    36301 gttgctgctt ttcttgcgca acaaatccgc tttacggata tcgctgcgtt gaatttatcc
    36361 gtactggaaa aaatggatat gcgcgaacca caatgtgtgg acgatgtgtt atctgttgat
    36421 gcgagcgcgc gtgaagtcgc cagaaaagag gtgatgcgtc tcgcaagctg aggataatcc
    36481 ggctacagag agtcgcgcta tttgttagcg tagggcttca gtgatatagt ctgcgccatc
    36541 tgatcgtaag tagttggctt tataaggtca ggtatgccgt ggttttacac ggcttttttt
    36601 tgtataggct tcagtattcc tgagtaccgt aaaccctgtc agggaataaa aaacgcgtga
    36661 tgttgtctgc tactcaacca cttagcgaaa aattgccagc gcatggctgc cgtcatgttg
    36721 cgatcattat ggacggcaat ggtcgctggg caaaaaagca agggaagatt cgtgcctttg
    36781 ggcataaagc cggagcaaaa tccgtccgcc gggctgtctc ttttgcggcc aacaacggta
    36841 ttgaggcgtt aacgctgtat gcctttagta gtgaaaactg gaaccgacca gcgcaggaag
    36901 tcagtgcgtt aatggaactg tttgtgtggg cgctcgatag cgaagtaaaa agtctgcacc
    36961 gacataacgt gcgtctgcgt attattggcg ataccagtcg ctttaactcg cgtttgcaag
    37021 aacgtattcg taaatctgaa gcgctaacag ccgggaatac cggtctgacg ctgaatattg
    37081 cggcgaacta cggtggacgt tgggatatag tccagggagt caggcaactg gctgaaaagg
    37141 tgcagcaagg aaacctgcaa ccagatcaga tagatgaaga gatgctaaac cagcatgtct
    37201 gtatgcatga actggcccct gtagatttag taattaggac tgggggggag catcgcatta
    37261 gtaacttttt gctttggcaa attgcctatg ccgaacttta ctttacagat gttctctggc
    37321 ccgatttcga tgaacaagac tttgaagggg cgttaaatgc ctttgctaat cgagagcgtc
    37381 gtttcggcgg caccgagccc ggtgatgaaa cagcctgatg ggggtcgctt ttgctgaagt
    37441 atcgcctgat atctgctttt gtgttaatac ccgtcgtcat cgcggcgttg tttctgttgc
    37501 cgccggtggg gttcgccatt gtaacgctgg tggtctgcat gctggcagcg tgggaatggg
    37561 gacagcttag cggttttacc actcgttcgc agcgagtatg gttggcggtg ttatgcgggt
    37621 tattgttggc gctgatgctt tttctgttgc cggaatatca ccgaaatatt catcaaccgc
    37681 tggttgaaat ctcactttgg gcttcgctgg gttggtggat tgtcgcgcta ttgctggtgc
    37741 tgttttaccc aggttccgca gcaatctggc gtaactctaa aacattgcgc cttatttttg
    37801 gcgtgctaac cattgttccc ttcttctggg gcatgctggc gttacgggcc tggcactatg
    37861 acgagaatca ttacagtggc gcaatatggc tgctctatgt catgatcctg gtatggggcg
    37921 ctgactccgg cgcatatatg tttggcaaat tgtttggtaa acataagctg gcaccgaagg
    37981 tttctccggg taaaacctgg caaggcttta tcggtggact cgctactgca gcggtaatct
    38041 catggggtta tggcatgtgg gcgaatctcg acgtcgctcc cgtcacctta ctcatttgct
    38101 ctattgtcgc agcgttagcc tcagtgctcg gcgatctgac cgagagtatg tttaagcgtg
    38161 aagcaggaat taaggacagc ggtcatttaa ttccaggaca cggtggtatt ttagatcgta
    38221 ttgatagcct gacggctgcg gtaccggtct ttgcttgctt gttgttactg gtattcagga
    38281 cgctttaacg gaaggtaata tgctgagttt tctctgggat ttggcttcgt tcatcgttgc
    38341 actgggtgta cttatcaccg tgcatgaatt tggtcatttc tgggttgccc ggcgttgtgg
    38401 tgttcgcgtt gagcgtttct caatagggtt tggtaaggcg ctctggcggc gaactgataa
    38461 gctcggcacc gaatatgtta tcgccctgat cccgttgggc ggttatgtca aaatgctgga
    38521 tgagcgcgca gaaccggtcg ttccggaact ccgccaccat gccttcaata ataaatctgt
    38581 cggccaacga gcggcgatta ttgccgcagg tccggttgca aacttcattt ttgctatctt
    38641 tgcctactgg ctggttttta ttattggtgt gcctggcgta cgtccggtgg ttggcgaaat
    38701 agcagccaat tcgatagctg cggaagcaca aattgcacca ggtacggaac taaaagccgt
    38761 agatggtatc gaaacgcctg attgggatgc cgtgcgtttg cagttggtcg ataaaattgg
    38821 cgatgaaagc accaccatta cagtagcgcc atttggcagc gaccaacggc gggatgtaaa
    38881 gctcgattta cgtcactggg cgtttgagcc tgataaagaa gatccggtat cttcgctggg
    38941 gattcgtcct cgtgggccgc aaattgaacc tgtactggaa aatgtgcagc caaactcggc
    39001 ggcaagcaag gcaggtttgc aagcaggcga caggatcgtt aaagtcgatg gtcagccctt
    39061 aacgcagtgg gtgacctttg tgatgcttgt ccgggataac ccgggtaaat ccttagcgtt
    39121 agaaatcgaa aggcagggga gtcccttgtc tttgacatta atcccggaga gtaaaccggg
    39181 taatggtaaa gcgattggtt ttgtcggtat tgagccgaaa gtcattcctt tgccagatga
    39241 gtataaagtt gtacgccagt atgggccgtt caacgccatc gtcgaagcca cggacaaaac
    39301 gtggcagctg atgaagctga cggtcagtat gctgggaaaa ttgatcaccg gtgatgtgaa
    39361 actgaacaac ctcagtgggc cgatctctat cgccaagggg gctgggatga cagcggaact
    39421 cggggtagtt tattacctgc cgtttcttgc gcttattagc gtgaacttag ggataattaa
    39481 cctgtttccg ttgcccgtac ttgacggggg gcatctgctg ttccttgcga tcgaaaagat
    39541 caaaggcgga ccggtatccg agcgggttca agacttttgt tatcgcattg gctcgattct
    39601 gctggtgctg ttaatggggc ttgcactttt caatgatttc tctcggttat gagagttagt
    39661 taggaagaac gcataataac gatggcgatg aaaaagttgc tcatagcgtc gctgctgttt
    39721 agcagcgcca ccgtatacgg tgctgaaggg ttcgtagtga aagatattca tttcgaaggc
    39781 cttcagcgtg tcgccgttgg tgcggccctc ctcagtatgc cggtgcgcac aggcgacacg
    39841 gttaatgatg aagatatcag taataccatt cgcgctctgt ttgctaccgg caactttgag
    39901 gatgttcgcg tccttcgtga tggtgatacc cttctggttc aggtaaaaga acgtccgacc
    39961 attgccagca ttactttctc cggtaacaaa tcggtgaaag atgacatgct gaagcaaaac
    40021 ctcgaggctt ctggtgtgcg tgtgggcgaa tccctcgatc gcaccaccat tgccgatatc
    40081 gagaaaggtc tggaagactt ctactacagc gtcggtaaat atagcgccag cgttaaagct
    40141 gtcgtgaccc cgctgccgcg caaccgtgtt gacctaaaac tggtgttcca ggaaggtgtg
    40201 tcagctgaaa tccagcaaat taacattgtt ggtaaccatg ctttcaccac cgacgaactg
    40261 atctctcatt tccaactgcg tgacgaagtg ccgtggtgga acgtggtagg cgatcgtaaa
    40321 taccagaaac agaaactggc gggcgacctt gaaaccctgc gcagctacta tctggaccgc
    40381 ggttatgccc gtttcaacat agactctacc caggtcagtc tgacgccaga taaaaaaggt
    40441 atttacgtca cggtgaacat caccgaaggc gatcagtaca agctttctgg cgttgaagtg
    40501 agcggcaacc ttgccgggca ctccgctgaa attgagcagc tgactaagat cgagccgggc
    40561 gagctgtata acggcaccaa agtgaccaaa atggaagatg acattaaaaa gcttctcggt
    40621 cgctatggtt atgcctatcc gcgcgtacag tcgatgcctg aaattaacga tgccgacaaa
    40681 accgttaaat tacgcgtgaa cgttgatgcg ggtaaccgtt tctacgtgcg taagatccgt
    40741 tttgaaggta acgatacctc gaaagatgcc gtcctgcgtc gcgaaatgcg tcagatggaa
    40801 ggtgcatggc tgggaagcga tctggtcgat cagggtaagg agcgtctgaa tcgtctgggc
    40861 ttctttgaaa ctgtcgatac cgatacccaa cgtgttccgg gtagcccgga ccaggtagat
    40921 gtagtctaca aggtgaaaga acgtaacact ggtagcttca acttcgggat tggttacggt
    40981 actgaaagtg gcgtgagctt ccaggctggc gtgcaacagg ataactggtt aggtacaggt
    41041 tatgctgttg gtatcaacgg gaccaaaaac gattaccaga cctatgctga actgtcggta
    41101 accaacccgt acttcaccgt agatggcgta agcctcggtg gtcgtctctt ctataatgac
    41161 ttccaggcag atgacgccga cctgtccgac tataccaaca agagttatgg tacagacgtg
    41221 acgttgggct tcccgattaa cgaatataac tcgctgcgtg caggtctggg ttatgtacat
    41281 aactccctgt ccaacatgca gcctcaggtt gcgatgtggc gttatctgta ctctatgggt
    41341 gaacatccga gcacctctga tcaggataac agcttcaaaa cggacgactt cacgttcaac
    41401 tatggttgga cctataacaa gcttgaccgt ggttacttcc cgacagatgg ttcacgtgtc
    41461 aacctgaccg gtaaagtgac cattcctgga tcggataacg aatactacaa agtgacgtta
    41521 gacacggcga cttatgtgcc gatcgatgac gatcacaaat gggttgttct ggggcgtacc
    41581 cgctggggtt atggtgatgg tttaggcggc aaagagatgc cgttctacga gaacttctat
    41641 gccggtggtt ccagcaccgt gcgtggcttc cagtccaata caattggtcc gaaagcagtt
    41701 tacttcccgc atcaggccag taattatgat ccggactatg attacgaatg tgcgactcag
    41761 gacggcgcga aagacctgtg taaatcggat gatgctgtag gcggtaacgc catggcggtt
    41821 gccagcctcg agttcatcac cccgacgccg tttattagcg ataagtatgc taactcggtt
    41881 cgtacttcct tcttctggga tatgggtacc gtttgggata caaactggga ttccagccaa
    41941 tattctggat atccggacta tagtgatcca agcaatatcc gtatgtctgc gggtatcgca
    42001 ttacaatgga tgtccccatt ggggccgttg gtgttctcct acgcccagcc gttcaaaaag
    42061 tacgatggag acaaggcaga acagttccag tttaacatcg gtaaaacctg gtaagtgttc
    42121 tccacaaagg aatgtagtgg tagtgtagcg atgactttag gcgatcaata taaatcgccg
    42181 ggccacgcaa agaactgcac cctccggtgc aaatgggatg gtaaggagtt tattgtgaaa
    42241 aagtggttat tagctgcagg tctcggttta gcactggcaa catctgctca ggcggctgac
    42301 aaaattgcaa tcgtcaacat gggcagcctg ttccagcagg tagcgcagaa aaccggtgtt
    42361 tctaacacgc tggaaaatga gttcaaaggc cgtgccagcg aactgcagcg tatggaaacc
    42421 gatctgcagg ctaaaatgaa aaagctgcag tccatgaaag cgggcagcga tcgcactaag
    42481 ctggaaaaag acgtgatggc tcagcgccag acttttgctc agaaagcgca ggcttttgag
    42541 caggatcgcg cacgtcgttc caatgaagaa cgcggcaaac tggttactcg tatccagact
    42601 gctgtgaaat ccgttgccaa cagccaggat atcgatctgg ttgttgatgc aaacgccgtt
    42661 gcttacaaca gcagcgatgt aaaagacatc actgccgacg tactgaaaca ggttaaataa
    42721 gtaatgcctt caattcgact ggctgattta gcgcagcagt tggatgcaga actacacggt
    42781 gatggcgata tcgtcatcac cggcgttgcg tccatgcaat ctgcacaaac aggtcacatt
    42841 acgttcatgg ttaacccaaa ataccgtgag catttaggct tgtgccaggc gtccgcggtt
    42901 gtcatgaccc aggacgatct tcctttcgcg aaaagtgccg cgctggtagt gaagaatccc
    42961 tacctgactt acgcgcgcat ggcgcaaatt ttagatacca cgccgcagcc cgcgcagaac
    43021 attgcaccca gtgcggtgat cgacgcgacg gcgaagctgg gtaacaacgt atcgattggc
    43081 gctaacgcgg tgattgaatc cggcgttgaa ctgggcgata acgtgattat cggtgccggt
    43141 tgcttcgtag gtaaaaacag caaaatcggt gcaggttcgc gtctctgggc gaacgtaacc
    43201 atttaccatg agatccagat cggtcagaat tgcctgatcc agtccggaac agtggtaggc
    43261 gcagacggct ttggttatgc caacgatcgt ggtaactggg tgaagatccc acagattggt
    43321 cgcgtaatta ttggcgatcg cgtggagatc ggtgcctgca ctaccatcga tcgcggcgcg
    43381 ctggatgaca ctgttattgg caatggcgtg atcattgata accagtgcca gattgcacat
    43441 aacgtcgtga ttggcgacaa tacggcggtt gccggtggcg tcattatggc gggcagcctg
    43501 aaaattggtc gttactgcat gatcggcgga gccagcgtaa tcaacgggca tatggaaata
    43561 tgcgacaaag tgacggttac gggcatgggt atggtgatgc gtcccatcac tgaaccaggc
    43621 gtctattcct caggcattcc gctgcaaccc aacaaagtct ggcgcaaaac cgctgcactg
    43681 gtgatgaaca ttgatgacat gagcaagcgt ctgaaatcgc ttgagcgcaa ggttaatcaa
    43741 caagactaac gttccatctt ttgttcgcca aactttacgg cctgtctcat tcttacgatt
    43801 gcggcaggcc gtgttattat tgtcgtttct tatattttga caggaagagt atcttgacta
    43861 ctaacactca tactctgcag attgaagaga ttttagaact tctgccgcac cgtttcccgt
    43921 tcttactggt ggatcgcgtg ctggattttg aagaaggtcg ttttctgcgc gcagtaaaaa
    43981 atgtctctgt caatgagcca ttcttccagg gccatttccc tggaaaaccg attttcccgg
    44041 gtgtgctgat tctggaagca atggcacagg caacaggtat tctggcgttt aaaagcgtag
    44101 gaaaactgga accgggtgag ctgtactact tcgctggtat tgacgaagcg cgcttcaagc
    44161 gcccggtcgt gcctggcgat caaatgatca tggaagtcac tttcgaaaaa acgcgccgcg
    44221 gcctgacccg ttttaaaggg gttgctctgg tcgacggtaa agtagtttgc gaagcaacga
    44281 tgatgtgtgc tcgtagccgg gaggcctgat acgtgattga taaatccgcc tttgtgcatc
    44341 caaccgccat tgtggaagag ggcgcgtcga ttggcgcgaa cgcacacatt ggtccttttt
    44401 gtatcgttgg accccatgtc gaaattggtg agggtaccgt actgaaatct cacgttgtcg
    44461 tgaatggtca tactaaaatt ggccgcgata atgagattta tcagttcgcc tccatcggcg
    44521 aagttaacca ggatctgaaa tatgctggcg aaccgacccg cgtggaaatc ggcgatcgta
    44581 accgcattcg cgaaagcgtc accattcatc gtggcacagt ccagggcggt ggattgacga
    44641 aggtgggcag cgacaactta ctgatgatca acgcgcacat tgcgcacgat tgtacggtag
    44701 gtaaccgctg tattctcgcc aacaacgcaa cgctggcggg tcacgtatcg gttgacgact
    44761 tcgcgatcat cggcggcatg gccgcagtcc atcagttctg catcattggt gcgcatgtga
    44821 tggttggcgg ctgctccggt gtggcgcagg acgtccctcc ttatgtcatt gcgcagggta
    44881 accacgccac gccgttcggt gtcaatatcg aagggctgaa gcgccgcgga ttcagccgtg
    44941 aggcgattac cgctatccgc aatgcgtata agctgattta tcgtagcggt aaaacgctcg
    45001 atgaagtgaa accggaaatt gctgaactgg cggaaacata tccggaagtg aaagccttta
    45061 ccgatttctt tgcacgctca acgcgcggtc tgattcgtta atgactgaac agcgtccatt
    45121 aacgattgcc ctggtcgccg gagaaacctc cggcgatatc ctgggggccg gtttaatccg
    45181 cgctctgaaa gaacgtgtgc ccaacgcccg ctttgttggt gttgccgggc cacgaatgca
    45241 ggctgaaggc tgcgaagcct ggtacgaaat ggaagagttg gcggtgatgg gcattgttga
    45301 agtgctcggt cgtctgcgtc gcttactgca tattcgtgcc gatctgacaa agcgttttgg
    45361 tgaactaaag ccagatgttt ttgttggcat tgatgcgcct gacttcaata ttatcctcga
    45421 aggtaacctt aaaaagcagg gtatcaaaac cattcattat gtcagtccgt ccgtctgggc
    45481 gtggcgacag aaacgcgttt tcaaaatagg cagagccacc gatctggtgc tcgcatttct
    45541 gcctttcgaa aaagcgtttt atgacaaata caacgtaccg tgccgcttta tcggtcatac
    45601 catggctgat gccatgccat tagatccaga taaaaatagt gcccgtgatg tgctggggat
    45661 cccttacgat gcccactgtc tggcattgtt gccgggcagc cgtggtgcag aagtcgaaat
    45721 gcttagtgcc gatttcctga aaacggccca gcttttgcgc cagacatatc cggatctcga
    45781 aatcgtggtg ccactggtga atgccaaacg ccgcgagcag tttgaacgca tcaaagctgc
    45841 agtcgcgcca gacctgtcgg ttcatttgct ggatggaatg ggccgtgagg cgatggtcgc
    45901 cagcgatgcg gcactactgg cgtcggggac ggcagccctg gagtgtatgc tggcgaaatg
    45961 cccgatggtg gtgggatatc gcatgaagcc ttttaccttc tggttggcga agcggctggt
    46021 gaaaactgat tatgtctcgc tgccaaatct gctggcgggc agagagttag tcaaagagtt
    46081 attgcaggaa gagtgtgagc cgcaaaaact ggctgcggcg ctgttaccgc tgttggcgaa
    46141 tgggaaaacc agccacgcga tgcacgatac cttccgtgaa ctgcatcagc agatccgctg
    46201 caatgccgat gagcaggcgg cacaagccgt tctggagtta gcacaatgat cgaatttgtc
    46261 tatccgcaca cgcagctggt tgcgggtgtg gatgaagtcg gacgcgggcc gttagttggc
    46321 gcggtcgtca ccgctgcggt gattctcgac ccggcgcgcc cgattgccgg gctgaatgat
    46381 tccaaaaagc tgagcgaaaa acgtcgtctg gcgctctgtg aagagatcaa agagaaagcg
    46441 ttgagctgga gtctgggccg cgcggaacct cacgaaatcg acgaactgaa tattttacat
    46501 gcgaccatgc tggcgatgca gcgagccgtc gctgggctgc atattgcgcc ggaatatgtg
    46561 ttgattgatg gtaaccgctg cccgaaatta ccgatgcctg cgatggctgt ggtgaaaggc
    46621 gatagccgcg taccggaaat cagtgccgcg tctatcctgg cgaaagtgac gcgtgacgcc
    46681 gaaatggcgg cgctggatat tgttttcccg caatatggtt ttgcccaaca caaagggtat
    46741 ccaaccgctt ttcatctgga aaaactggct gaacacggcg cgaccgaaca ccatcggcgc
    46801 agctttgggc ctgtcaaacg cgcactggga cttgcgtcct gattcttgtg tcgagattaa
    46861 gtaaaccgga atctgaagat gtctgaacca cgtttcgtac acctgcgggt gcacagcgac
    46921 tactcgatga tcgatggcct ggccaaaacc gcaccgctgg taaaaaaggc ggcggcgttg
    46981 ggtatgccag cactggcgat caccgatttc accaaccttt gcggtctggt gaagttctac
    47041 ggagcgggac atggcgcagg gattaagcct atcgtcgggg cagattttaa cgtccagtgc
    47101 gacctgctgg gtgatgagtt aacccacctg acggtactgg cggcgaacaa taccggctat
    47161 cagaatctga cgttgctgat ctcaaaagcg tatcagcgcg ggtacggtgc cgccgggccg
    47221 atcatcgatc gcgactggct tatcgaatta aacgaagggt tgatccttct ttccggcggg
    47281 cgcatgggcg acgtcggacg cagtcttttg cgtggtaaca gcgcgctggt agatgagtgt
    47341 gtcgcgtttt atgaagaaca cttcccggat cgctattttc tcgagctgat ccgcaccggc
    47401 aggccggatg aagaaagcta tctgcacgcg gcggtggaac tggcggaagc gcgcggtttg
    47461 cccgtcgtgg cgactaacga tgtgcgcttt atcgacagca gcgactttga cgcacacgaa
    47521 atccgcgtcg cgatccacga cggctttacc ctcgacgatc ctaaacgccc gcgtaactat
    47581 tcgccgcagc aatatatgcg tagcgaagag gagatgtgcg agctgtttgc cgacatccca
    47641 gaagcccttg ccaacaccgt tgagatcgcc aaacgctgta acgtaactgt gcgtcttggt
    47701 gaatacttcc tgccgcagtt cccgaccggg gacatgagca ccgaagatta tctggtcaag
    47761 cgtgcaaaag agggcctgga agagcgtctg gcctttttat tccctgatga ggaagaacgt
    47821 gttaagcgcc gcccggaata tgacgaacgt ctggagactg aacttcaggt tatcaaccag
    47881 atgggcttcc cgggctactt cctcatcgtt atggaattta tccagtggtc gaaagataac
    47941 ggcgtaccgg tagggccagg ccgtggctcc ggtgcgggtt cactggtggc ctacgcgctg
    48001 aaaatcaccg acctcgatcc gctggaattt gacctgctgt tcgaacgttt ccttaacccg
    48061 gaacgtgtct ccatgcctga cttcgacgtt gacttctgta tggagaaacg cgatcaggtt
    48121 atcgaacacg tagcggacat gtacggtcgt gatgcggtat cgcagattat caccttcggt
    48181 acgatggcgg cgaaagcggt gatccgcgac gtaggccgcg tgctggggca tccgtacggc
    48241 tttgtcgatc gtatctcgaa actgatcccg cccgatccgg ggatgacgct ggcgaaagcg
    48301 tttgaagccg agccgcagct gccggaaatc tacgaagcgg atgaagaagt taaggcgttg
    48361 atcgacatgg cgcgcaaact ggaaggggtc acccgtaacg ccggtaagca cgccggtggg
    48421 gtggttatcg cgccgaccaa aattaccgat tttgcgccgc tttattgcga tgaagagggc
    48481 aaacatccgg tcacccagtt tgataaaagc gacgttgaat acgccggact ggtgaagttc
    48541 gacttccttg gtttgcgtac gctcaccatc atcaactggg cgctggagat gatcaacaag
    48601 cggcgggcga agaatggcga gccgccgctg gatatcgccg cgatcccgct ggatgacaag
    48661 aaaagcttcg acatgctgca acgctcggaa accacggcgg tattccagct tgaatcgcgc
    48721 ggcatgaagg acctgatcaa gcgtctgcaa cctgactgct tcgaagatat gatcgcactg
    48781 gtggcactgt tccgccctgg tccgttgcaa tcagggatgg tggataactt tatcgaccgt
    48841 aaacatggtc gcgaagagat ctcctatccg gacgtgcagt ggcagcatga aagcctgaaa
    48901 ccggtactgg agccaaccta cggcattatc ctgtatcagg aacaggtcat gcagattgcg
    48961 caggtgcttt ctggttatac cctcggtggc gcggatatgc tgcgtcgtgc gatgggtaag
    49021 aaaaagccgg aagagatggc taagcaacgt tctgtatttg ctgaaggtgc agaaaagaac
    49081 ggaatcaacg ccgaactggc gatgaaaatc ttcgacctgg tggagaaatt cgctggttac
    49141 ggatttaaca aatcgcactc tgcggcctat gctttggtgt catatcaaac attatggttg
    49201 aaagcgcact atccggcaga gtttatggcg gcggtaatga ccgccgatat ggacaacacc
    49261 gagaaggtgg tgggcctggt ggatgagtgc tggcggatgg ggctgaaaat cctgccacca
    49321 gatataaact ccggtcttta ccatttccac gtcaacgacg acggcgaaat cgtgtatggt
    49381 attggcgcga tcaaaggggt aggtgaaggt ccgattgagg cgatcatcga agcccgtaat
    49441 aaaggcggct acttccgcga actgtttgat ctctgcgccc gaaccgacac caaaaagttg
    49501 aaccgtcgcg tgctggaaaa actgatcatg tccggggcgt ttgaccgtct tgggccacat
    49561 cgcgcggcgc tgatgaactc gctgggcgat gcgttaaaag cggcagatca acacgcgaaa
    49621 gcggaagcta tcggtcaggc cgatatgttc ggcgtgctgg ccgaagagcc ggaacaaatt
    49681 gaacaatcct atgccagctg ccaaccgtgg ccggagcagg tggtattaga tggggaacgt
    49741 gaaacgttag gcctgtacct gaccggacac cctatcaacc agtatttaaa agagattgag
    49801 cgttatgtcg gaggcgtaag gctgaaagac atgcacccga cagaacgtgg taaagtcatc
    49861 acggctgcgg ggctcgttgt tgccgcgcgg gttatggtca ccaagcgcgg caatcgtatc
    49921 ggtatctgca cgctggatga ccgttccggg cggctggaag tgatgttgtt tactgacgcc
    49981 ctggataaat accagcattt gctggaaaaa gaccgcatac ttatcgtcag cggacaggtc
    50041 agctttgatg acttcagcgg tgggcttaaa atgaccgctc gcgaagtgat ggatattgac
    50101 gaagcccggg aaaaatatgc tcgcgggctt gctatctcgc tgacggacag gcaaattgat
    50161 gaccagcttt taaaccgact ccgtcagtct ctggaacccc accgctctgg gacaattcca
    50221 gtacatctct actatcagag ggcggatgca cgcgcgcggt tgcgttttgg cgcgacgtgg
    50281 cgtgtctctc cgagcgatcg tttattaaac gatctccgtg gcctcattgg ttcggagcag
    50341 gtggaactgg agtttgacta atacaggaat actatgagtc tgaatttcct tgattttgaa
    50401 cagccgattg cagagctgga agcgaaaatc gattctctga ctgcggttag ccgtcaggat
    50461 gagaaactgg atattaacat cgatgaagaa gtgcatcgtc tgcgtgaaaa aagcgtagaa
    50521 ctgacgcgta aaatcttcgc cgatctcggt gcatggcaga ttgcgcaact ggcacgccat
    50581 ccacagcgtc cttataccct ggattacgtt cgcctggcat ttgatgaatt tgacgaactg
    50641 gctggcgacc gcgcgtatgc agacgataaa gctatcgtcg gtggtatcgc ccgtctcgat
    50701 ggtcgtccgg tgatgatcat tggtcatcaa aaaggtcgtg aaaccaaaga aaaaattcgc
    50761 cgtaactttg gtatgccagc gccagaaggt taccgcaaag cactgcgtct gatgcaaatg
    50821 gctgaacgct ttaagatgcc tatcatcacc tttatcgaca ccccgggggc ttatccgggc
    50881 gtgggcgcag aagaacgtgg tcagtctgaa gccattgcac gcaacctgcg tgaaatgtct
    50941 cgcctcggcg taccggtagt ttgtacggtt atcggtgaag gtggttctgg cggtgcgctg
    51001 gcgattggcg tgggcgataa agtgaatatg ctgcaataca gcacctattc cgttatctcg
    51061 ccggaaggtt gtgcgtccat tctgtggaag agcgccgaca aagcgccgct ggcggctgaa
    51121 gcgatgggta tcatcgctcc gcgtctgaaa gagctgaaac tgatcgactc catcatcccg
    51181 gaaccgctgg gcggcgctca ccgtaacccg gaagcgatgg cggcatcgtt gaaagcgcaa
    51241 ctgctggcgg atctggccga tctcgacgtg ttaagcactg aagatttaaa aaatcgtcgt
    51301 tatcagcgcc tgatgagcta cggttacgcg taattcgcaa aagttctgaa aaagggtcac
    51361 ttcggtggcc cttttttatc gccacggttt gagcaggcta tgattaagga aggattttcc
    51421 aggaggaaca catgaacatc attgccatta tgggaccgca tggcgtcttt tataaagatg
    51481 agcccatcaa agaactggag tcggcgctgg tggcgcaagg ctttcagatt atctggccac
    51541 aaaacagcgt tgatttgctg aaatttatcg aacataaccc tcgaatttgc ggcgtgattt
    51601 ttgactggga tgaatacagt ctcgatttat gtagcgatat caatcagctt aatgaatatc
    51661 tcccgcttta tgccttcatc aacacccact cgacgatgga tgtcagcgtg caggatatgc
    51721 ggatggcgct ctgttttttt gaatatgcgc tggggcaggc ggaagatatc gccattcgta
    51781 tgcgtcagta caccgacgaa tatcttgata acattacccc accgttcacg aaagccttgt
    51841 ttacctacgt caaagagcgg aagtacacct tttgtacgcc gggtcatatg ggcggcaccg
    51901 catatcaaaa aagcccggtt ggctgtctgt tttatgactt tttcggcggg aataccctta
    51961 aggctgatgt ctctatttcg gtcaccgagc ttggttcgtt gctcgaccac accgggccac
    52021 acctggaagc ggaagagtac atcgcgcgga ctttcggcgc ggaacagagt tatatcgtta
    52081 ccaacggaac atcgacgtcg aacaaaattg tgggtatgta cgccgcgcca tccggcagta
    52141 cgctgttgat tgaccgcaat tgtcataaat cgctggcgca tctgttgatg atgaacgatg
    52201 tagtgccagt ctggctgaaa ccgacgcgta atgcgttggg gattctgggt gggatcccgc
    52261 gccgtgaatt tactcgcgac agcatcgaag agaaagtcgc tgccaccacg caggcacaat
    52321 ggccggttca tgcggtgatt accaactcaa cctatgatgg cttgctctac aacaccgact
    52381 ggatcaaaca gacgctggat gtcccgtcga tccacttcga ttccgcctgg gtgccgtaca
    52441 cccattttca tccgatctac caggggaaaa gtggtatgag cggcgagcgt gttgcgggaa
    52501 aagtgatctt cgaaacgcaa tcgacccaca aaatgctggc ggcgttatcg caggcttcgc
    52561 tgatccacat taaaggtgag tatgacgaag aggcgtttaa cgaagccttt atgatgcata
    52621 ccaccacctc gcccagttat cccattgttg cttcggttga gacggcagca gcgatgctgc
    52681 gtggtaatcc gggcaaacgg ctgattaacc gttcagtaga acgagctctg cattttcgca
    52741 aagaggtcca gcggctgcgg gaagagtctg acggttggtt tttcgatatc tggcaaccgc
    52801 cgcaggtgga tgaagccgaa tgctggcccg ttgcgcctgg cgaacaatgg cacggcttta
    52861 acgatgcgga tgccgatcat atgttcctcg atccggttaa agtcactatt ctgacgccgg
    52921 ggatggacga gcagggcaat atgagcgagg aggggatccc ggcggcgctg gtggcgaagt
    52981 tcctcgatga gcgtgggatc gtggtagaga aaaccggccc atataacctg ctgtttctct
    53041 ttagtattgg catcgataaa accaaagcaa tgggattatt gcgtgggttg acggaattta
    53101 aacgctctta cgatctcaat ctgcgaatca aaaatatgct tccggatctg tacgcagaag
    53161 atcccgattt ctaccgcaat atgcgtattc aggatctggc gcaagggatc cataagctga
    53221 ttcgtaaaca cgatcttccc ggtttgatgt tgcgggcatt cgatactttg ccggagatga
    53281 tcatgacgcc acatcaggca tggcagagac agattaaagg tgaagtagaa accattgcgc
    53341 tggaacaact ggtcggtaga gtatcggcaa atatgatcct gccttatcca ccgggcgtac
    53401 cgctgttgat gcctggagaa atgctgacca aagagagccg cacagtactc gattttctac
    53461 tgatgctttg ttccgtaggg caacattacc ccggttttga aacggatatt cacggtgcga
    53521 aacaggacga agacggcgtt taccgcgtac gagtcctaaa aatggcggga taacttgcca
    53581 gagcggcttc cgggcgagta acgtgctgtt aacaaataaa ggagacgtta tgctgggttt
    53641 aaaacaggtt caccatattg cgattattgc gacggattat gcggtgagca aagctttcta
    53701 ctgcgatatt cttggtttca cgctgcaaag cgaagtctat cgcgaagcgc gcgactcgtg
    53761 gaaaggggat ttggcgctta atgggcaata tgtgattgag cttttctcat ttccgttccc
    53821 gccggagcgc cccagccgac cggaagcttg cggcctgcgt catctggctt ttagcgttga
    53881 tgacatcgat gcggcagtgg cgcaccttga aagccataac gtgaagtgtg aagccatccg
    53941 tgtcgatcca tacacgcaaa aacgcttcac cttctttaac gatccggacg ggctgccgtt
    54001 ggaactgtat gagcagtaag gcttgtcatc gccgcatttg cccggtaacg tgccgggcat
    54061 tgctactgta aaatcgcacc atcatgacac tcacgctcaa tagacaactt ctcacctcac
    54121 gccagattct ggtggccttt agcggcgggc ttgactccac cgttctgctg catcagttgg
    54181 tgcagtggcg gacggaaaat ccggggggcg ctctgcgcgc tattcatgtg catcacggtt
    54241 taagtgccaa tgccgatgcc tgggttacgc attgcgaaaa cgtctgccaa cagtggcagg
    54301 tgccgctggt ggtcgaacgc gtacaacttg cgcaagaggg gctggggatt gaggcccagg
    54361 cgcggcaggc gcgttatcag gcatttgccc gcaccttgtt gcccggtgaa gtgctggtca
    54421 ccgcgcaaca tctcgacgat caatgtgaaa cttttctgct ggcgctaaaa cgcggtagcg
    54481 gccctgccgg gctttcggct atggcggaag tttcggagtt tgccggaaca cggcttatcc
    54541 gcccgttgct tgcccgcacg cggggggaac tggcgcagtg ggcattggcg catggtttac
    54601 gctggattga agacgaaagt aatcaggacg actcatacga tcgtaacttt ctgcgcctgc
    54661 gcgtagtgcc gttattgcag cagcgttggc cgcattttgc cgaaacaacg gcccgcagcg
    54721 ccgcactttg tgctgaacaa gagagcctgc tggatgaact gctggcagat gatttagcac
    54781 actgtcaatc gccgcagggg acgctgcaga ttgtgccaat gctggcgatg agtgatgccc
    54841 gccgcgcggc gattatccgc cgctggctgg cagggcagaa tgcaccgatg ccttcccgcg
    54901 acgcgttggt gaggatctgg caggaagtgg cgctggcgcg ggaagatgcc tccccctgtt
    54961 tacgtttggg cgcgtttgaa atccgacgct atcagtcgca actgtggtgg attaaatccg
    55021 tcaccgggca aagcgaaaac attgtgccgt ggcagacatg gcttcaaccg ctggaattac
    55081 cggcggggca gggaagtgta cagcttaatg cgggaggcga tattcgccct ccgcgtgcag
    55141 acgaagcggt cagcgtgcgt ttcaaagcgc caggattgct gcatattgtc gggcgtaacg
    55201 gcggacgtaa gctaaagaaa atctggcaag agctgggcgt gccgccgtgg ctacgtgaca
    55261 ccacgccact gctgttttat ggcgaaacgc tgattgcggc ggcaggggga tttgtgacgc
    55321 aagaaggtgt ggctgaaggt gagaatggca taagttttgt ctggcagcga aacgcttagt
    55381 ttagtgaagg ccggatatga cgcattaagc gtcgcatccg gcgaaagtca atcaggactc
    55441 gctcaccacc accgtaccga tttccgggtg gctaaagctg gtaattttat ccagacgtag
    55501 ctcacgggtt tcgccagcgg cctcgacgac cagatactcc acatttttgc gggagactaa
    55561 atcgctggct ttggcctgca atttttcgcc atctttcagc tcaagtgtca gcattaaatg
    55621 atgctggcag gcgagctcaa gattatcgta atcatcacaa ttgattggtt gatacgtatc
    55681 attcattgac ataatcgctc accagtaagt ttgccgcagc gtatgctgct ttttccctga
    55741 cagcctcaga aagggcgtcg tcggcagcca tttcattcag cactttcaaa acgcagccca
    55801 gcgcgtccgg aacgtatcct aagtctccgc tggcgatttc cgcgtaccgc ttgcgtatta
    55861 actcacaata tttttccaca tgccctcctg tcagcactct gacttaaccg tggatgcaag
    55921 tctaagccta cgaagataaa ctctgtttcg caaggtgact ataccacact catttctgca
    55981 atatcagcgc cgcaactgca cgtattccgt tacaatggcc tcctgattcg aaaggagttt
    56041 tcttatggcg cttaaagcga caatttataa agcgacggtt aatgtggccg atctcgaccg
    56101 caaccagttt ctcgatgcct ctctgacgct ggcgcgccac ccttcagaaa cccaggagcg
    56161 tatgatgctg cgcctgctgg cgtggctgaa atatgctgat gaacgtctgc aatttacccg
    56221 tggtttgtgt gccgatgatg agccggaagc gtggctgcgt aacgatcacc tgggcattga
    56281 tttgtggatt gagctggggc tgccggatga gcgacgaatt aagaaagcct gcacccaggc
    56341 cgcagaagtg gcgctgttta cctataatag tcgggcggcg caaatctggt ggcagcaaaa
    56401 tcagagcaaa tgtgcgcagt ttgccaatct ttccgtctgg tatctggacg acgagcagct
    56461 ggcgaaagtg agcgcctttg ccgatcgtac catgacgctg caggcaacga ttcaggatgg
    56521 cgtgatctgg ttatcggatg ataagaataa tctggaagtg aacttaaccg cctggcaaca
    56581 accttcatga ttgtgatttc ccgacatgtt gctattcccg atggtgagct tgagatcacc
    56641 gccattcgtg cgcagggcgc gggcgggcag catgttaata agacctcaac ggccattcat
    56701 ctgcgttttg acattcgggc atccagcctg ccagagtatt acaaagagcg tctgctcgcc
    56761 gccagccatc atttgatcag cagtgatggc gtaattgtca ttaaggcaca ggaataccgc
    56821 agtcaggaac tgaaccgcga agcggcgctg gcccggctgg tggcagtgat taaagattta
    56881 acaacagaac aaaaagcccg acgacccacg cggcccaccc gtgcatcgaa agagcgcagg
    56941 ctggcatcga aagcacaaaa atcaagcgtg aaggcgatgc gcagcggtcg ggaataaaaa
    57001 gaaggaatgg atggtgaaaa aagcgatagt gacagcgatg gctgtaatca gcctctttac
    57061 tctgatgggt tgtaataatc gggccgaagt cgatacgctt tctccggcgc aggctgccga
    57121 attgaaaccg atgccgcaaa gttggcgcgg cgtgctgccg tgtgccgatt gcgaaggaat
    57181 cgaaacctct ctgttcctcg aaaaagacgg aacatgggtg atgaatgagc gttatctcgg
    57241 tgctcgtgaa gaaccttcct ccttcgcttc ctacggtaca tgggcgcgaa ccgctgacaa
    57301 gctggtatta accgacagca aaggtgaaaa gtcatattat cgggcgaaag gcgatgcgct
    57361 ggagatgctc gatcgtgaag gcaatccgat tgaatcgcag ttcaactata cgctggaacc
    57421 ggcacaatcc agtttaccca tgacgccgat gaccctgcgg ggcatgtatt tttatatggc
    57481 tgatgcggcg accttcactg attgcgcgac cggaaaacgt ttcatggtag cgaataacgc
    57541 agagctggag cgtggctacc tggctgcgcg cggccacagt gaaaaaccgg tgttactgtc
    57601 agtagaaggt cactttacgc ttgaggctaa tccggatacc ggtgcgccaa ccaaagtatt
    57661 agcacccgat acggcaggga aattttaccc aaaccaggat tgcagtagtt tggggcagta
    57721 acccgtcttg agacagaaac aaacgcaaaa caggccagaa ggatatattt caacattttg
    57781 aatttgcacg ttttttgtag gccggataag gcgtttacgc cgcatccggc aatggtgctc
    57841 aacgcctgat gcgacgctgg cgcgtcttat catgcctaca acccccctca taccttaagg
    57901 cggataaggc atttacttta tcaccgggtc aaaccaacaa agcgacccgc cttgatataa
    57961 atccctggct tcaggtgccc aacatactgt aactgcgttt ctggcttaaa cgcagagaca
    58021 tcaccaatac gcatatgcat cagatcggca gggctaatcc agcccgattg cgccagcgta
    58081 agcggatgcc cggctttggc aaatgcatca gtgacaaatt ccgaacaaaa ccacgacttt
    58141 ttgtctcctt cgaccacact gcttaactgc gctttcgcca ggccgctgac gcactgttgg
    58201 cgaaaatcct cggagaacgg attcagtgag cacatctggc gagtcaccat aaagggaata
    58261 aattcgacaa tgccgcgata gttataaccg ctatctttga ttttattggc gaacgcggtg
    58321 atttctgtgg cttgttgcgg ggtaagatcc gggactcgta agacgaaaag tttatcacta
    58381 tgcttcatcg cttttttaag ggaaacaatc tggacgccag cgcctgtcgc ttctgcaacg
    58441 ttattttcac ccagatagat tgcaacgtga ctcacagagg aagtgctgaa aacgcggatc
    58501 ccaaatgagg ttacctccag gcttgaggag aacagcaaat cgccgggttt gagatcaggt
    58561 accgttattt cttttattga ttgttcggtg aaagagcttt gatgctggaa tttgacagcc
    58621 catgttttcg cctcggcatc tactgctgtg gctgatgagt ctggctggct gatatcaacg
    58681 gtacaggccg aaagtaaaag aaagcagggg agaagcaggc ggcagtacgc ctttggttta
    58741 tccattttat acaatccatg taaaaaaggg ccctgaaatt caggaccctt tctggcatca
    58801 gcctttaatc tgtttcacca gatattcgac gatgtcacca gtcttaatta actgtttctc
    58861 gccgttgcga cgatatttat attcgatatc gtcgttgtcg aggttacggt cgcccagcac
    58921 aatagtgtgc ggaataccga tcagttccat atcggcaaac atcacgcccg gacgttcttt
    58981 tcggtcatcc agcagcactt cgataccttg tgcacgcagt tcgctgtaca gtttctcagc
    59041 aagctcttgt acgcggaagg atttgtgcat gttcatcggc agaattgcca cctggaacgg
    59101 cgcgatagcg tcaggccata cgatgccgcg ctcgtcgtag ttctgctcaa tcgccgcagc
    59161 cactacgcgc gttaccccga taccgtagca acccatagtc aggatttggt tacggccatc
    59221 ttcaccctgt acggaggctt tcagtgcttc ggagtacttg gtacccagct ggaagatgtg
    59281 accaacttcg ataccacgtt tgatcagcag cgtaccctgg ccatccgggc ttggatcgcc
    59341 agccaccacg ttacggatat ccgcaacttc cggggtagcg acatcgcgat cccagttgat
    59401 gccgaagtag tgtttaccat cgatgttagc accagcagtg aaatcactca tcgccgcaac
    59461 ggtacggtca atcaccaccg gaatcggcat gtttaccgga cccagtgaac ccggaccggc
    59521 tttaaccacg gcacgaattt cttcttcggt cgcgaaagtc agcgggctgg caacctgcgg
    59581 cagtttttct gctttaactt cgttcagctc gtgatcaccg cgcaccagca gcgcaaccag
    59641 cgggaagcta ctgccttcaa ccgctttaac cagcagagtc ttaaccgttt tcttaatcgg
    59701 cagattgaac tgttcaacca gttccgcgat ggttttcgcg ttcggcgtat caaccagcgt
    59761 catttcctgg gtagcagcag cgcgcggttc tttcggcgcg atagcttctg ccagctcaat
    59821 gttcgctgca tagtcagagg tgtcggagaa gaccacatcg tcttcaccgc tctgcgccag
    59881 cacctggaat tcgtgagagg cgctgccgcc gatagaaccg gtgtcggcct gtacggcgcg
    59941 gaaatccagc cccatgcggc tgaagatttt gctgtaggcc gcatacattg catcgtaggt
    60001 ttcctgcagg gattcctgag aagtatggaa agagtaagca tctttcatca ggaattcgcg
    60061 ggaacgcatg acgccgaaac gcggacgcac ttcgtcgcgg aacttggtct ggatctgata
    60121 gaagttcagc ggcagctgtt tgtaagagct aagctcgtta cgaatcaggt cagtgataac
    60181 ttcttcatga gttgggccga gtacgaacgg acgctcgcca cggtcaacaa aacgcagcag
    60241 ttccggaccg tactgttccc aacgaccact ctcttgccac aaatcggctg gctgaaccac
    60301 cggcatcgac acctcgattg caccggcgtt gttcatctct tcacgcacga tgttttcgac
    60361 ttttttcaga acgcgcacgc cggtcggcag ccaggtatat aacccggagg ccagcttgcg
    60421 gatcatcccg gcgcgcagca tcagctgatg gctgatcacc tcggcgtcgg caggtgtctc
    60481 cttgagagtg gagagcaggt attggctagt acgcatgttg ttacggttcc agttggaagg
    60541 tagaacaggc tcaaggcgag cctgggacaa aaaaagtgat ttagtttacc agtgcaaaag
    60601 aaatgtcaaa agagaagggc gtgaatttaa cgcggttcca gcgcaaagac ttcaaaacct
    60661 gcgtcggtga cgcgccagcg aacgttaaaa tcatgtagcc agacggcata ggttttgccc
    60721 gtttcctcac ctttacgata ggccgggcgc gggtcctgcg ccagtacttc gcggataaac
    60781 agcgttaact gcggataacg cttctccagc gtcaaaagct gcttttcgac ctctgcggta
    60841 aaactcaccg ccatctctgc agctggcgca ctttgcgcat agctggcact ggcatcggga
    60901 agcgattcgg caaaggggag atacggtttg atatccacta ccggcgtacc atcgaccaga
    60961 tccaggctgc cgagcttcag aatcacgcag tctttatggc aaacaacctc tttcagctct
    61021 accagcgaca taccaatcgg gttggggcgg aaagtggagc gcgtagcgaa aacacccatt
    61081 ctggcattac cgccaagacg aggcggacgc acagtcggac gccagccgcc ttccatcgtt
    61141 tgatgaaaga cgaaaaggat ccataaatgg ctgaatgctt ccaggccgcg aacggcgtca
    61201 gcctggttgt agggagcaat gagatgtagt tcgccgttgg cgctttttac cagacctggc
    61261 tggcgcggaa cggcgaattt ttctttatag ggcgagcgaa taacacctat ttgctcgaac
    61321 tggaaactgc tcatttcgcc gtaatgttaa gcgcagaacc gatacataaa gcctgacgat
    61381 agcagcctgg cgtaccgctg gtaacttcgc agctatgcag taataccgca ttggctttca
    61441 ttttcgaggc gttgatttgc atccgcttac gcgcggttgg aatgctcggc ggagagtcct
    61501 gattagaggc ctggcaagag tcgccactga cttcaccgag atcgcggaac ggtttgccga
    61561 ctaattcttc tgcattggta taaattcgga ccggcgtggc gcgcggcgct ttcggttttg
    61621 caggctccgc tttcggctgg ggtgcagtgc tttgaacggg ttcgacaggg gatctgctta
    61681 acatggaaca gccgcttagc atgagtgcta ctaaacagat cggtaaagca cgcatagtat
    61741 ttcctcaatg tatgatcaaa acgtcaatat tgaatcagga gcttgtaaaa atgacaagac
    61801 gggcaagcgc ccgtcctgaa tgatattaca aattgtggaa acagcctaaa aattaccagc
    61861 ctttaacagc gccgccgtta aacactttgt ttgctgcttc gtaaacttcg tcagactgat
    61921 aagcctggac gaatttcttc acgttctcgg cgtctttgtt atcttcacgc gttacgatca
    61981 ggtttacgta cggggagtct ttatcttcga caaagatacc gtttttcgct ggagtcaggc
    62041 caatctggct ggcataggtg gtgttaataa ctgccagagc gatttgcgcg tcgtccagag
    62101 aacgcggcag ttgcggtgct tccagttcaa caattttcag attttttggg ttctcaacaa
    62161 catcaagaac ggtcggcagc aggccaacgc catctttcag tttgatcaag cccacttttt
    62221 gcagcagcag cagtgaacga ccaaggttag ttggatcgtt tggcacggca acctgcgaac
    62281 catcctgcag ttcatccagt gatttgattt tcttggagta accagcaatc ggataaacaa
    62341 aagtgttgcc tactgcgacc agtttgtagc cacgatcttt cagttgctga tcaaggtacg
    62401 gtttatgctg gaaggcgttg gcgtcgatat cgcctttgct caatgcttcg tttggcagaa
    62461 catagtcgtt gaaggttacc agctcaacgt ccaggccata tttgtctttc gcaactttct
    62521 gcgcgacttc tgcaacctgc tgttcggcac caacaatcac gccgacttta atgtggtttg
    62581 gatctttttc atcctgaccg cagcctacca gtgccagtga tccaatcagg gctcccactg
    62641 ccgcaaaggt tttgaatttg aacgccatac cttattcctt cttcaattat ttatgttgtg
    62701 ttgaacgtta cttgcgagtg acagcccgga cgatgcggtc gcctgcgaac tgaattaaat
    62761 aaaccagaat gaccagcaat accagtaccg tattcatcac cgtcgcgtta tagccgatgt
    62821 agccatactg atagccaatc tgacctaaac caccggcacc gactgcacca cccatcgcgg
    62881 aataaccgac cagggtaatc agggtgatag ttgccgcatt caccagaccc ggcaacgctt
    62941 ccggtaacag cactttacgg acgatctgca ttggcgtggc acccattgcg cgggaagctt
    63001 caattaaccc ggttgggatc tccagcagag cgttctcgac catacgggca ataaacggtg
    63061 ctgcaccaac ggttaacgga acaattgctg cctgcaaacc aatcgatgta ccgacaataa
    63121 cgcgggtaaa cggaatcatc cataccagca agataatgaa cgggatggaa cggaaaatgt
    63181 tcacaatcgc agaaacggta cgatacagct tcgcgttagc aataatttgc cccggacgcg
    63241 tgacataaag caggacgcca accggcagac caaccacaaa cccaaaaaat ccggatacga
    63301 aggtcattgc cagcgtttcc catacgccac gaaccagcag ccacatcatc ggctcagaca
    63361 taacccagta cctctacttt tacatggtgt tcctgcagcc aggcaatggc ggcttgcgta
    63421 tcttgttgtg tgccgtgcat ttcagtcagc atgatgccga acttaacgcc accggcgtaa
    63481 tccatctgcg cgctaataat gttgttgttg acgttgaaac gacgcgcggt ttcagaaagc
    63541 agtggggcat cgaccgattg accggtaaac tccagacgca gcatcggcac gcagtcagta
    63601 aatggctccg cttgcagacg ttcctggtaa tcttccggga tatccagatg cagggttgac
    63661 tgaataaact tctgcgccag cggcgttttc ggatgtgaga acacttcact taccgtgtcc
    63721 tgctcgatca gttcgccatt gctgatgacc gcaacgcaat cacaaatacg cttcacaacg
    63781 tccatttcgt gggtgatcaa cagaatcgtc aaacccagac ggcggttaat gtctttcagc
    63841 agttcgagaa tagaacgtgt cgttgccggg tctagcgcgc tggtggcttc atcacacagc
    63901 aatactttgg gattgctggc taatgcacgg gcaatcgcca cacgttgttt ctgcccaccg
    63961 gaaagattcg acgggtagct atcatgctta tctccaagac caaccaatga cagcaattcc
    64021 gtcacgcgac gtttgatctc gtctttcggt gtattgtcca gctccagcgg cagagccacg
    64081 ttgccaaaaa cagtacgcga agagagcagg ttaaaatgct ggaaaatcat accaatctgg
    64141 cggcgagctt tggtcaactc ggattctgac agcgtggtca gttcctgtcc atcgaccagc
    64201 acgctaccct cggttgggcg ctccagcagg tttacacaac gtataagcgt actcttaccc
    64261 gcgcctgagg caccgataac gccataaatt tgtccagctg gcacatgcag gctgacgttg
    64321 ttcaacgcct ggatggtgcg ggtgccctgg tggaacactt tggtgatatt cgaaagtttt
    64381 atcattgatt atttattatc gtcattaagt tagtcgtggc atctcgaatg cctgaaacgg
    64441 gcaacgccgt caatgaaatg gatgttaagg catccagacg tctaaatcaa tcaggtttat
    64501 gcgaagagca ctttcttgca ggtcgaaaca tgcgatacta gcgtcacatg ccttattaag
    64561 gagctataaa aggtggcgaa gagcgtaccc gcaatttttc ttgaccgtga tggcaccatt
    64621 aatgtcgatc acggctatgt ccatgagatc gacaactttg aatttatcga cggtgttatt
    64681 gacgccatgc gcgagctaaa aaaaatgggc tttgcgctgg tggtagtcac caaccagtct
    64741 ggcattgctc gcggtaaatt taccgaagca cagtttgaaa cgctgaccga gtggatggac
    64801 tggtcgctgg cggaccgaga tgtcgatctg gatggtatct attattgccc gcatcatccg
    64861 cagggtagtg ttgaagagtt tcgccaggtc tgcgattgcc gcaaaccaca tccggggatg
    64921 cttttgtcag cacgcgatta tttgcatatt gatatggccg cttcttatat ggtgggcgat
    64981 aaattagaag atatgcaggc agcggttgcg gcgaacgtgg gaacaaaagt gctggtgcgt
    65041 acgggtaaac ctattacacc tgaagcagaa aacgcggcag attgggtgtt aaatagcctg
    65101 gcagacctgc cgcaagcgat aaaaaagcag caaaaaccgg cgcaatgatt aaaagatgag
    65161 cggttgaaat aaaaatgcat ttttccgctt gtcttcctga gccgactccc tataatgcgc
    65221 ctccatcgac acggcgaatg tgaatcactt cacacaaaca gccggttcgg ttgaagagaa
    65281 aaaatcctga aattcagggt tgactctgaa agaggaaagc gtaatatacg ccacctcgcg
    65341 acagtgagct gaaagccgcg tcgcaactgc tctttaacaa tttatcagac aatctgtgtg
    65401 ggcactcgaa gat
//
LOCUS       DBFIFI010000007        65313 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.007, whole genome shotgun sequence.
ACCESSION   DBFIFI010000007 DBFIFI010000000
VERSION     DBFIFI010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65313
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.007"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            123..1820
                     /gene="ypdA"
                     /locus_tag="N7B39_000424"
     CDS             123..1820
                     /gene="ypdA"
                     /locus_tag="N7B39_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HGU2420476.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            1835..2569
                     /gene="ypdB"
                     /locus_tag="N7B39_000425"
     CDS             1835..2569
                     /gene="ypdB"
                     /locus_tag="N7B39_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HGU2420477.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            2582..3439
                     /gene="ypdC"
                     /locus_tag="N7B39_000426"
     CDS             2582..3439
                     /gene="ypdC"
                     /locus_tag="N7B39_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU2420478.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRLVEGEMLFIPARAANLPVNNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(3442..5937)
                     /gene="ptsP"
                     /locus_tag="N7B39_000427"
     CDS             complement(3442..5937)
                     /gene="ptsP"
                     /locus_tag="N7B39_000427"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HGU2420479.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIIRNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSEPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHGLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQAFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(5962..6999)
                     /gene="ypdE"
                     /locus_tag="N7B39_000428"
     CDS             complement(5962..6999)
                     /gene="ypdE"
                     /locus_tag="N7B39_000428"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HGU2420480.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHSAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFNYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(6999..8084)
                     /gene="ypdF"
                     /locus_tag="N7B39_000429"
     CDS             complement(6999..8084)
                     /gene="ypdF"
                     /locus_tag="N7B39_000429"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HGU2420481.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVLISR
                     ESAHILVDSRYYADVEARTQGYQLHLLDATHTLTTIARQIIADEQLQTLGFEGQQVSW
                     ETAHRWQSELNAKLVSATPDVLRQIKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKTSFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFNVYQIVLQAQLAAISAIRPGVRCQQVDEAARRV
                     ITEAGFSHYFGHNTAHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(8099..9346)
                     /gene="fryC"
                     /locus_tag="N7B39_000430"
     CDS             complement(8099..9346)
                     /gene="fryC"
                     /locus_tag="N7B39_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HGU2420482.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(9368..9694)
                     /gene="fryB"
                     /locus_tag="N7B39_000431"
     CDS             complement(9368..9694)
                     /gene="fryB"
                     /locus_tag="N7B39_000431"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HGU2420483.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(9913..10878)
                     /gene="glk"
                     /locus_tag="N7B39_000432"
     CDS             complement(9913..10878)
                     /gene="glk"
                     /locus_tag="N7B39_000432"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HGU2420484.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            11082..12338
                     /gene="yfeO"
                     /locus_tag="N7B39_000433"
     CDS             11082..12338
                     /gene="yfeO"
                     /locus_tag="N7B39_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HGU2420485.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMHQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            12453..12779
                     /gene="ypeC"
                     /locus_tag="N7B39_000434"
     CDS             12453..12779
                     /gene="ypeC"
                     /locus_tag="N7B39_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HGU2420486.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(12919..14157)
                     /gene="mntH"
                     /locus_tag="N7B39_000435"
     CDS             complement(12919..14157)
                     /gene="mntH"
                     /locus_tag="N7B39_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HGU2420487.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            14493..15695
                     /gene="nupC"
                     /locus_tag="N7B39_000436"
     CDS             14493..15695
                     /gene="nupC"
                     /locus_tag="N7B39_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HGU2420488.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ACEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WIGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(15745..17934)
                     /gene="pdeA"
                     /locus_tag="N7B39_000437"
     CDS             complement(15745..17934)
                     /gene="pdeA"
                     /locus_tag="N7B39_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HGU2420489.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSIYRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEVLARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(18142..18217)
                     /locus_tag="N7B39_000438"
     tRNA            complement(18142..18217)
                     /locus_tag="N7B39_000438"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18182..18184),aa:Ala,seq:ggc)
     gene            complement(18257..18332)
                     /locus_tag="N7B39_000439"
     tRNA            complement(18257..18332)
                     /locus_tag="N7B39_000439"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18297..18299),aa:Ala,seq:ggc)
     gene            18553..18912
                     /gene="yfeC"
                     /locus_tag="N7B39_000440"
     CDS             18553..18912
                     /gene="yfeC"
                     /locus_tag="N7B39_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HGU2420490.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            18914..19306
                     /gene="yfeD"
                     /locus_tag="N7B39_000441"
     CDS             18914..19306
                     /gene="yfeD"
                     /locus_tag="N7B39_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HGU2420491.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLLEAEEPLAEYAPGIRTPAYRQIISAIDNMTH
                     SEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(19346..20554)
                     /locus_tag="N7B39_000442"
     CDS             complement(19346..20554)
                     /locus_tag="N7B39_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HGU2420492.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDAPSQPLQQSLKELERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYLNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKKLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCTANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQ
                     EPTEMIQATA"
     gene            20562..20987
                     /gene="tnpA"
                     /locus_tag="N7B39_000443"
                     /pseudo
     CDS             20562..20987
                     /gene="tnpA"
                     /locus_tag="N7B39_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(21105..22520)
                     /gene="gltX"
                     /locus_tag="N7B39_000444"
     CDS             complement(21105..22520)
                     /gene="gltX"
                     /locus_tag="N7B39_000444"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HGU2420493.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYFQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALAFIAERENQQ"
     gene            22779..22854
                     /locus_tag="N7B39_000445"
     tRNA            22779..22854
                     /locus_tag="N7B39_000445"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22812..22814,aa:Val,seq:tac)
     gene            22899..22974
                     /locus_tag="N7B39_000446"
     tRNA            22899..22974
                     /locus_tag="N7B39_000446"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22932..22934,aa:Val,seq:tac)
     gene            23021..23096
                     /locus_tag="N7B39_000447"
     tRNA            23021..23096
                     /locus_tag="N7B39_000447"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23054..23056,aa:Val,seq:tac)
     gene            23101..23176
                     /locus_tag="N7B39_000448"
     tRNA            23101..23176
                     /locus_tag="N7B39_000448"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23134..23136,aa:Lys,seq:ttt)
     gene            23296..23637
                     /locus_tag="N7B39_000449"
     CDS             23296..23637
                     /locus_tag="N7B39_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HGU2420494.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQHI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQRQQAEKAQEKEQRLSLNVSLLNP
                     VENTTHIGIYI"
     gene            complement(23628..24554)
                     /gene="yfeR"
                     /locus_tag="N7B39_000450"
     CDS             complement(23628..24554)
                     /gene="yfeR"
                     /locus_tag="N7B39_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU2420495.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     VILSEPFFLLCHRDSALAAEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNTLMAGREGDPLYQI"
     gene            24644..25642
                     /gene="yfeH"
                     /locus_tag="N7B39_000451"
     CDS             24644..25642
                     /gene="yfeH"
                     /locus_tag="N7B39_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HGU2420496.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(25639..25857)
                     /gene="ypeB"
                     /locus_tag="N7B39_000452"
     CDS             complement(25639..25857)
                     /gene="ypeB"
                     /locus_tag="N7B39_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HGU2420497.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(25859..27874)
                     /gene="ligA"
                     /locus_tag="N7B39_000453"
     CDS             complement(25859..27874)
                     /gene="ligA"
                     /locus_tag="N7B39_000453"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HGU2420498.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KSNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTYCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(27945..28943)
                     /gene="zipA"
                     /locus_tag="N7B39_000454"
     CDS             complement(27945..28943)
                     /gene="zipA"
                     /locus_tag="N7B39_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HGU2420499.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSA
                     PQPAQQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLN
                     SIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQV
                     PSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            29173..29934
                     /gene="cysZ"
                     /locus_tag="N7B39_000455"
     CDS             29173..29934
                     /gene="cysZ"
                     /locus_tag="N7B39_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HGU2420500.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            30119..31090
                     /gene="cysK"
                     /locus_tag="N7B39_000456"
     CDS             30119..31090
                     /gene="cysK"
                     /locus_tag="N7B39_000456"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HGU2420501.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            31474..31731
                     /gene="ptsH"
                     /locus_tag="N7B39_000457"
     CDS             31474..31731
                     /gene="ptsH"
                     /locus_tag="N7B39_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HGU2420502.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            31776..33503
                     /gene="ptsI"
                     /locus_tag="N7B39_000458"
     CDS             31776..33503
                     /gene="ptsI"
                     /locus_tag="N7B39_000458"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HGU2420503.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            33544..34053
                     /gene="crr"
                     /locus_tag="N7B39_000459"
     CDS             33544..34053
                     /gene="crr"
                     /locus_tag="N7B39_000459"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HGU2420504.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(34096..34947)
                     /gene="pdxK"
                     /locus_tag="N7B39_000460"
                     /gene_synonym="thiJ"
     CDS             complement(34096..34947)
                     /gene="pdxK"
                     /locus_tag="N7B39_000460"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HGU2420505.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMLVVVVTADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            35052..35419
                     /gene="yfeK"
                     /locus_tag="N7B39_000461"
                     /pseudo
     CDS             35052..35419
                     /gene="yfeK"
                     /locus_tag="N7B39_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
     gene            complement(35422..36333)
                     /gene="cysM"
                     /locus_tag="N7B39_000462"
     CDS             complement(35422..36333)
                     /gene="cysM"
                     /locus_tag="N7B39_000462"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HGU2420506.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPNNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMADRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITYFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(36467..37564)
                     /gene="cysA"
                     /locus_tag="N7B39_000463"
     CDS             complement(36467..37564)
                     /gene="cysA"
                     /locus_tag="N7B39_000463"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HGU2420507.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIEPRDEELALAQSA"
     gene            complement(37554..38429)
                     /gene="cysW"
                     /locus_tag="N7B39_000464"
     CDS             complement(37554..38429)
                     /gene="cysW"
                     /locus_tag="N7B39_000464"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HGU2420508.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEENHEH"
     gene            complement(38429..39262)
                     /gene="cysT"
                     /locus_tag="N7B39_000465"
     CDS             complement(38429..39262)
                     /gene="cysT"
                     /locus_tag="N7B39_000465"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HGU2420509.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(39262..40278)
                     /gene="cysP"
                     /locus_tag="N7B39_000466"
     CDS             complement(39262..40278)
                     /gene="cysP"
                     /locus_tag="N7B39_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HGU2420510.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            complement(40436..41227)
                     /gene="ucpA"
                     /locus_tag="N7B39_000467"
     CDS             complement(40436..41227)
                     /gene="ucpA"
                     /locus_tag="N7B39_000467"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HGU2420511.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(41356..42213)
                     /gene="murR"
                     /locus_tag="N7B39_000468"
     CDS             complement(41356..42213)
                     /gene="murR"
                     /locus_tag="N7B39_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HGU2420512.1"
                     /translation="MLYLTKIRNAESEFTENEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATAQHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGCDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            42377..43273
                     /gene="murQ"
                     /locus_tag="N7B39_000469"
     CDS             42377..43273
                     /gene="murQ"
                     /locus_tag="N7B39_000469"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HGU2420513.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNIGLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPVVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            43277..44701
                     /gene="murP"
                     /locus_tag="N7B39_000470"
     CDS             43277..44701
                     /gene="murP"
                     /locus_tag="N7B39_000470"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HGU2420514.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PDIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPLGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            44706..46010
                     /gene="pbp4b"
                     /locus_tag="N7B39_000471"
     CDS             44706..46010
                     /gene="pbp4b"
                     /locus_tag="N7B39_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HGU2420515.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMAQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDLIAKYISGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     THWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(46068..46967)
                     /gene="yfeX"
                     /locus_tag="N7B39_000472"
     CDS             complement(46068..46967)
                     /gene="yfeX"
                     /locus_tag="N7B39_000472"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HGU2420516.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNIWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(47063..47638)
                     /gene="yfeY"
                     /locus_tag="N7B39_000473"
     CDS             complement(47063..47638)
                     /gene="yfeY"
                     /locus_tag="N7B39_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HGU2420517.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADAVDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(47699..48148)
                     /gene="yfeZ"
                     /locus_tag="N7B39_000474"
     CDS             complement(47699..48148)
                     /gene="yfeZ"
                     /locus_tag="N7B39_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HGU2420518.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWRVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEVKE"
     gene            complement(48135..48560)
                     /gene="ypeA"
                     /locus_tag="N7B39_000475"
     CDS             complement(48135..48560)
                     /gene="ypeA"
                     /locus_tag="N7B39_000475"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HGU2420519.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            48774..49643
                     /gene="amiA"
                     /locus_tag="N7B39_000476"
     CDS             48774..49643
                     /gene="amiA"
                     /locus_tag="N7B39_000476"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HGU2420520.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            49647..50546
                     /gene="hemF"
                     /locus_tag="N7B39_000477"
     CDS             49647..50546
                     /gene="hemF"
                     /locus_tag="N7B39_000477"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HGU2420521.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREGGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGKDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(50552..51604)
                     /gene="eutR"
                     /locus_tag="N7B39_000478"
     CDS             complement(50552..51604)
                     /gene="eutR"
                     /locus_tag="N7B39_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HGU2420522.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(51650..52150)
                     /gene="eutK"
                     /locus_tag="N7B39_000479"
     CDS             complement(51650..52150)
                     /gene="eutK"
                     /locus_tag="N7B39_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HGU2420523.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGVTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(52163..52822)
                     /gene="eutL"
                     /locus_tag="N7B39_000480"
     CDS             complement(52163..52822)
                     /gene="eutL"
                     /locus_tag="N7B39_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HGU2420524.1"
                     /translation="MPALDLIRPSVTAMRVIASVNADFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAQDTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(52832..53719)
                     /gene="eutC"
                     /locus_tag="N7B39_000481"
     CDS             complement(52832..53719)
                     /gene="eutC"
                     /locus_tag="N7B39_000481"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HGU2420525.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCATTTCAAPVTSESCA
                     LDLGSAEAKVWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTLALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(53740..55101)
                     /gene="eutB"
                     /locus_tag="N7B39_000482"
     CDS             complement(53740..55101)
                     /gene="eutB"
                     /locus_tag="N7B39_000482"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HGU2420526.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELCSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     CIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(55113..56516)
                     /gene="eutA"
                     /locus_tag="N7B39_000483"
     CDS             complement(55113..56516)
                     /gene="eutA"
                     /locus_tag="N7B39_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HGU2420527.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKSLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDVRSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALIQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(56513..57739)
                     /gene="eutH"
                     /locus_tag="N7B39_000484"
     CDS             complement(56513..57739)
                     /gene="eutH"
                     /locus_tag="N7B39_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HGU2420528.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKDDATAAKTEAEAQS"
     gene            complement(57839..59026)
                     /gene="eutG"
                     /locus_tag="N7B39_000485"
     CDS             complement(57839..59026)
                     /gene="eutG"
                     /locus_tag="N7B39_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HGU2420529.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQLVGLYA
                     AAQ"
     gene            complement(59016..59852)
                     /gene="eutJ"
                     /locus_tag="N7B39_000486"
     CDS             complement(59016..59852)
                     /gene="eutJ"
                     /locus_tag="N7B39_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HGU2420530.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTFAGNRRISLEEAEQYKRGHGEEIWPTVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            complement(59863..61266)
                     /gene="eutE"
                     /locus_tag="N7B39_000487"
     CDS             complement(59863..61266)
                     /gene="eutE"
                     /locus_tag="N7B39_000487"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HGU2420531.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     IAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAGKIA
                     AAIGLKVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAVDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(61278..61565)
                     /gene="eutN"
                     /locus_tag="N7B39_000488"
     CDS             complement(61278..61565)
                     /gene="eutN"
                     /locus_tag="N7B39_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HGU2420532.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPHGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(61672..61965)
                     /gene="eutM"
                     /locus_tag="N7B39_000489"
     CDS             complement(61672..61965)
                     /gene="eutM"
                     /locus_tag="N7B39_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HGU2420533.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(62004..63020)
                     /gene="pta"
                     /locus_tag="N7B39_000490"
     CDS             complement(62004..63020)
                     /gene="pta"
                     /locus_tag="N7B39_000490"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HGU2420534.1"
                     /translation="MIIERCCELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            complement(63017..63748)
                     /gene="eutT"
                     /locus_tag="N7B39_000491"
     CDS             complement(63017..63748)
                     /gene="eutT"
                     /locus_tag="N7B39_000491"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HGU2420535.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPLGCQAIVGLSDEDLHRLSHQPLRYLGH
                     DHLVPEASHGRDAALLNLLRTKVREAETVAAQVFITRSFEVLRPDILQALNRLSSTVY
                     VMMILSVTKQPLTVKQIQQRLGETQ"
     gene            complement(63745..64446)
                     /gene="eutQ"
                     /locus_tag="N7B39_000492"
     CDS             complement(63745..64446)
                     /gene="eutQ"
                     /locus_tag="N7B39_000492"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HGU2420536.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDELIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAQPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(64421..64900)
                     /gene="eutP"
                     /locus_tag="N7B39_000493"
     CDS             complement(64421..64900)
                     /gene="eutP"
                     /locus_tag="N7B39_000493"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HGU2420537.1"
                     /translation="MKRIAFVGTVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(64913..65248)
                     /gene="eutS"
                     /locus_tag="N7B39_000494"
     CDS             complement(64913..65248)
                     /gene="eutS"
                     /locus_tag="N7B39_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HGU2420538.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
BASE COUNT        15364 a        18022 c        16544 g        15383 t
ORIGIN      
        1 ctaatacacc atttatctat caatcccctt ttaaaattgt ggtttttcct caaaaacggc
       61 ctgctggtca tcagccttca ggttacctat catagaggtt taatccttat tcagagtcac
      121 ccgtgcacga aatattcaac atgctgctgg cggtcttcga tcgggcagcg ttaatgctta
      181 tctgcctgtt ctttctcatc cgtatccgcc tgtttcgcga actgttgcac aagtcggcac
      241 actcaccgaa agagttgctc gccgttaccg ccattttctc gctgttcgcc ctcttcagca
      301 cctggtccgg cgttcccgta gaaggctcgc tggtgaacgt acgtattatc gcggtgatgt
      361 ccggcgggat tctgtttggc ccgtgggtgg gcatcattac cggcgtgatt gcgggtattc
      421 atcgttattt aattgatatc ggtggcgtga cggcgatccc ctgctttatc accagcattc
      481 tggctggttg tatatcgggc tggatcaacc tgaaaatccc caaagcacag cgctggcgcg
      541 tcggtattct cggcggcatg ttgtgtgaaa cgttgaccat gattctggtg attgtctggg
      601 caccaactac cgcgttgggg atcgatatcg tctctaaaat cgggattcca atgatcctcg
      661 gcagcgtctg tatcggcttt attgtgcttc tagtgcaaag cgttgagggc gaaaaagagg
      721 ccagcgccgc gcggcaggcc aagttggcgc tggatatcgc caacaaaacg ctacctctgt
      781 ttcgccatgt caatagcgag tcattacgca aagtttgtga aattattcgc gatgacattc
      841 acgccgatgc ggtggcgatt accaataccg atcatgtgct ggcctatgtt ggtgtgggtg
      901 aacataacta tcagaatggc gatgacttca ttagcccgac tacccgtcag gcgatgaatt
      961 acggaaaaat catcattaaa aacaatgatg aagcccaccg cacaccagag attcattcca
     1021 tgctggtgat cccattgtgg gagaaaggcg ttgtgaccgg aacgctgaaa atttactact
     1081 gccacgcgca tcagataacc tcgtcattac aggaaatggc ggttggtctg tcgcaaatca
     1141 tctccacgca actggaggtt tcacgcgccg agcagctacg tgaaatggca aataaggcag
     1201 agcttcgcgc cctgcaaagc aaaattaatc cccatttcct gtttaacgct ttgaacgcta
     1261 tttcatcgtc gatccgtctg aatccggata ccgctcgcca gctgatcttt aatctgtcgc
     1321 gttatctgcg ctataacatt gaattaaaag acgatgagca aatcgatatc aaaaaagagc
     1381 tgtatcaaat taaagactat attgccattg agcaggcccg ctttggcgac aagctgacgg
     1441 ttatctatga tattgatgaa gaggtgaatt gctgcattcc cagcctgctg atccagccgt
     1501 tggtggagaa tgccattgtc cacggtatcc agccttgcaa aggtaaaggc gttgtcacca
     1561 tcagcgttgc agagtgcggc aatcgggtac gcattgcggt gcgggatacc gggcacggca
     1621 tcgatccaaa ggtgattgag cgggtcgaag cgaatgaaat gccgggcaat aaaattggcc
     1681 tgctgaatgt ccatcatcgc gtgaagttat tgtatggcga ggggctgcat atccgccgcc
     1741 tggaaccagg aacggaaatt gcgttttaca ttcctaacca acgcacccca gtcgcctcac
     1801 aggctacgtt attgctttga gccggagtga tattgtgaaa gtcatcattg ttgaagacga
     1861 attcctggca caacaggaac tgagctggct aattaaagag cacagccaga tggagattgt
     1921 cggcaccttt gacgacggtc tggacgtgtt gaagtttttg cagcataacc gcgtcgacgc
     1981 catttttctg gatatcaata ttccgtcgct ggatggtgtg ttgctggcac aaaacatcag
     2041 ccagttcgcc cataaaccgt ttattgtgtt catcaccgcg tggaaagaac atgcggtaga
     2101 agcgtttgaa ctggaggcgt ttgactacat tctaaaaccg tatcaggagt cacgcattac
     2161 cgggatgctg caaaaactgg aagcagcctg gcaacaacag cagaccagca gcacgcctgc
     2221 cgcgacggta acgcgtgaga atgacaccat taatctggtg aaagatgagc gaataatcgt
     2281 cacgccaatt aacgatatct attacgccga agcgcacgag aaaatgacct ttgtttatac
     2341 gcggcgtgaa tcctacgtaa tgccgatgaa cattaccgaa ttttgcagca aactgccgcc
     2401 gtcgcatttt ttccgctgcc atcgctcatt ttgtgtcaat ctgaacaaaa tccgcgaaat
     2461 cgaaccgtgg tttaataaca cctatattct gcggctgaaa gatctggatt ttgaagtgcc
     2521 ggtcagccgc agcaaagtta aagaattccg ccagttaatg catctttaat cgggaacttt
     2581 catgaaagca ccaggcttgc ccgccgatca acaatttttc gccgatctgt tcagcggcct
     2641 ggtgcttaac ccgcaactac tggggcgcgt ctggtttgcc agccagcctg cctcgttgcc
     2701 ggtgggcagt ttatgtattg attttccccg tctggatatc gtgctgcgcg gcgaatacgg
     2761 caatctgctg gaagcaaagc agcaacgttt ggtggaagga gaaatgctgt ttattccggc
     2821 gcgcgcggct aatttaccgg tcaacaacaa accggtgatg ctgttaagcc tggtgttcgc
     2881 tccgacctgg cttgggttat cgttttacga tagccgcacc acgtcattgt tgcatcctgc
     2941 tcgccagatc cagcttccca gcctgcaacg cggtgaaggt gaagcgatgc ttaccgccct
     3001 cacccatctt agccgttcgc cgctggagca aaatatcatt cagccgctgg tgttaagttt
     3061 gctgcatctt tgccgtaacg tggtgaatat gccgccgggc aattcgcagc cgcgcggcga
     3121 ttttctctat cacagcattt gtaactgggt tcaggataat tatgcccagc cgctcacccg
     3181 cgagagcgtg gcgcagtttt ttaatatcac gcccaatcat ctgtcaaaac tgtttgctca
     3241 gcatggaacg atgcgtttta tcgagtatgt gcgttgggtg cgaatggcga aggcgaggat
     3301 gattttgcag aaatatcatt tgtcaattca tgaagtggca cagcgttgcg gttttccgga
     3361 tagcgactat ttttgtcgcg ttttccggcg tcagtttggt ctgacgccgg gagagtacag
     3421 cgcccgtttt cagggctaac gtcagaaggt taattctgtt tccagcagcg tcaggatact
     3481 ttgtgcatct tgcgcggcaa acaacgactg gcggaagttt ttattcacca gtttacgcgc
     3541 cagctgcgag aagactttca catgattcat cccttcgtta gcacccagcg tcagcatgat
     3601 caccagttcg acttcgccca tttctgactg ccagtcaacc ggtttcgcca gccgggcaat
     3661 gctgatactg gaatgacgga tccactgaga tttggtgtgc gggatcgcca cgccaaaacc
     3721 aacgccagtg gtgacaatct cttcccgctg ccagacatcc tcttccagct cgaacggatg
     3781 ttcagtgcgc ccgttaacgc cgaggttacc gcacaggaac tggatcgcct gctctttatt
     3841 gctaaaggcc tgatcaacga agatattctc cagcgccagc agcgggcgaa cgtcttcttc
     3901 cggcgtaaag gcggtgagta acgcttcaat ttcctgcgcg ctgcgacatt cacacgcctg
     3961 acgtgccagt tcccgacacg cctcgctatc cagttgacga agctggcttt tcaccgccgg
     4021 aatgcgcggg ctactcatac tcagctcatc caggcccagc ccaagcagta gcggcagata
     4081 acggctttca ccgcccagtt caccgcaaat gcctacccat ttgccccgct gatgcgcagt
     4141 ggtaactatt tgctgcaaca tgcgcaggaa cgatggcgta atcgggttat atagcggcga
     4201 tacgcgcggg ttattacgat cgaccgcata cagatactgg gtcatatcgt tggagccgat
     4261 actgaagaaa tcgacctcat cgcagaagtg atcgatgatg tagcacaccg acggcacttc
     4321 caccatgata cccagcgtaa tcgtctctgc atgacgcagg ccatcgcgct taagctcaac
     4381 gatcgctttt tggatctcgc ccttcaccca taagatctga tcgaggccgt ggaccatcgg
     4441 gatcatcaac tgggcgttgc cgaaactggc ggcgcgcaaa atggcccgca gttgagtgcg
     4501 gaacaggcca gcaaattccg ggtaaatacg taccgcgcga tagccgagga acgggttctc
     4561 ttcctgggga atattcagat aaggaatgct tttatcgcct ccgatgtcca ttgtgcggaa
     4621 gataatcggc ttgtcgcccg ctgccagtag cacctgctgg taggcttcaa attgctcctg
     4681 ctcgtccggc tcgctgtcgc gatccatata gagcatttcg gtacggaaca gcccgacgcc
     4741 ttccgcgccg ttggcaaacg cgccaggcgc ttccagagcg gtgccaatat tcgccgcgat
     4801 atcaatacgc ttgttatcac gggaataagc cagctgcgcg gcagcctgcg cctgttgttt
     4861 ttggcgttta tccgccagcg tctgcgcgac ctgataataa ccgctcaccg cgtcattcgg
     4921 gttaatcgcc agcacgccgc actgggcgtc aagcactgca ggttgcccgg cataacgcgc
     4981 gatcgcatcc agcggcaggc cgctcagtac cgggatcgcc gaggcgcggg ccagaatcag
     5041 ggtatgcgag gtgcgcccgg ttttctccag aatcatgccc gcaagatttt ttaaatccag
     5101 gcttaaaaac tggcttgggg ttaaatcctc ggccaccaga atagtcggtt tttccagcac
     5161 cagattgttg cgcggcttca gttccggcca ggtaatatgc agcaactgtt cgctaatatc
     5221 gcgaatgtcg ctaacacgtt cgcgcagata atcgctggca gaagcagaaa gtttggcgca
     5281 aacctgctcc atatttctga tgatcgccgc ccccagcccc tgatgctgtt ctgtcatcag
     5341 gcgacggata ttgcctgcaa attcatcatc ctgaatcagc gacaaatggg cgctgaggat
     5401 agttttgctt tcgccgtcac gctcacgcaa ttgctggttc agttgctcgg caagcgttgc
     5461 caggctgtgc tccagccggg tggaatcttg cgcactggcg gggattgccc gataactgtc
     5521 gaggctgtcg ctttgtaaca gggtcagcgt acccacgccg acgccgcttg ccagcacatt
     5581 gccgtacagt aaatccgggt taaggcggct taatgaacgc ggcagcggat gcgccgtcag
     5641 ttctgcctgc gtaggctgaa cgctgtcgct gtcgataaag cgcacctgga tgtactcttc
     5701 cagcacgcgc cgcgcctgct cttcatcgct gccgctaatg ttcaggctgc aactgtcatt
     5761 aaatagggtg ccggtgccaa tcagcgccag cgagcttttg gcatcggcct ttgcgttctg
     5821 gcgatggtta ataaaagtga tttcgctttg ccactggctg cactgttctt taagttccca
     5881 cgccggacga gcatgtagac cgttaggcaa aggacagaga aattgaatcg ttaacataac
     5941 cgctccgggt taatcaggag atcatctgaa atccgtcagt tgaacaaccg tctcacgcgt
     6001 aagacgttga ataagggcag ataaaagttg ctccatctgc aaaatgtcac ggcaatcggc
     6061 aatcgacgcg gcgcaatgtc cgtggcgggt ggcaggcccc atcaccactg tgggtacgcc
     6121 agtaccggtt aagtgcaccg ctccaccgtc cgtgccgccg ttactgaaca tatccgcctg
     6181 taacggcacg ccaatttctg ccgccacggt ttcgatccag gcggtgagtt ttggcggcgc
     6241 aatcagtgac ttgtcgctta acaccagcat cgggccgtta ccaatctggc gatggttagc
     6301 cgcgccataa ttaaagtttt tcgcccagca ggcggtatca aggacaatgg cgacgtccgg
     6361 cgacaccgcg cgggtggcag tttgcccgcc gcgtaacccc acctcttcgc tggaactggc
     6421 caccagccac acttccgcag gcagttcagc gctgtgtagt tcgcgcagta acgtcaccag
     6481 caggtagcaa ccgaggcggt catcaaaggc tttccccatc acccgctggt gggggagaac
     6541 ctgaaaagtg gtatcgaacg tgacgcgatc gcctggacga attcccgcct gcatcacttc
     6601 gtcatacgag cgcgcgccaa tatccacgcg catggcgctg acatcattcc cctgccggtc
     6661 gccgtcaagc aggcctggaa ttttgcactc ttcacgggtg gtgatgcgca ccggctgcag
     6721 ctggcgggca gccatgcgta cattgccaac cggcagcaca tcaatcgccc cttcgcggga
     6781 gatgctgcgc accataaatc ccacttcgtc catatgcgca cagatcatca ccttcggacc
     6841 tgtcgattca ttgaggcgga tcagcaccga tcccagccca tcaaatcgca cttctttttg
     6901 caggcgatcc gcttcttcca gcaggatctg ccgcacttcc tgttccgagg aggcgatcgc
     6961 atctgcctcg ctcaacgctt tcaatagcga taaatccatt atgcctctcc cgtgagcaac
     7021 actgttttcg gcatggcgta gagcacttct gcgccttgcg gggtgaccag cacaacatcc
     7081 tcgatgcgca cgcccccttg ccctggcaaa taaatccccg gctccacggt cagtaacatg
     7141 cctggctgta gcgtcgtggt gtcccgcggt gaaaaacgcg gatcttcatg aacttcaatg
     7201 ccgatagcat gagcggtgtt atgaccgaaa tagtggctaa aacctgcctc ggtaatcacc
     7261 cgacgcgcgg cttcgtcaac ctgctggcag cgcacgccgg ggcgaattgc agagattgct
     7321 gcgagctgtg cctgcaaaac aatctgatag acgttaaaca gcgggtgaga ttcggcgctc
     7381 accccttcgc cattcaccag caaggtgcgc gtcatatcag agcagtagcc ctgatagagc
     7441 gcaccgaaat cgagagtgac aaactcgccc gctgcaacaa tcttgtcgct ggctttgccg
     7501 tgcggcagcg ccccacgcca gccactggcg acaatggtgt caaaagaggt tttttctgcg
     7561 ccctgctggc gcataaacca ctccagttca gcggctatct cgcgctcgct catccccgcc
     7621 tgaataaagc ggcgaatatg ctctgcaccg cgatcggcaa tcccacaggc aaggcggatt
     7681 ttctccacct cctctggcgt tttgatttgc cgcagcacat ccggcgtggc gctaaccagt
     7741 ttcgcattga gttcagactg ccagcgatgc gcggtttccc aactcacctg ctggccttca
     7801 aaaccgagcg tttgcaactg ctcatcggca atgatttgcc tggcgatggt tgtaagcgtg
     7861 tgcgtcgcgt caagcaaatg cagctggtaa ccttgcgtgc gggcttctac atccgcgtaa
     7921 tagcgcgagt ccaccagaat gtgcgcactt tcacggctaa tcagcacata gcctgatccg
     7981 gtggagatcc ccagatgcgg ctgtttgttc tgccgtgagg agagaagcac cgcatccagt
     8041 tgttgcgcct taagccagtc gcgcagcgaa gcgagtaatg tcatctggat tttccttctt
     8101 acaggctatc gattaacaat ttgcctttac ggaacatcat cagacgcagg aacaccacca
     8161 tcagcgcagt tatgactgcc cccagcgcga tccccgccat atagacgccc aggttagtga
     8221 ccagcggcca tgcccagata gcggattccg ggaaccattg cactgcgccc agccagacag
     8281 cggcggttga gccgacaatc gcgccgacca tatacgacgg aatggcggtg atggggcttt
     8341 ccagcgcaaa cggaatcgca ccctcactga tccccataaa ggcaaggaac atcgcggtct
     8401 taccctgcgg ataaagttgt gcgttgaaca ggcgtttgcc ggttaaacga cggtcaataa
     8461 tggtcgccag acccagacca atcggcggaa taacgatagc gatagaacga gcggtgaccg
     8521 gtaatacgtg gtcagtggta aagctgaagg caacaaaacc tgcggctttg ttgatcgggc
     8581 caccaaggtc gattgctgtc gccgcggcga tccccattgc gtacatcagc gcgcccttct
     8641 cacctgcggc agtcagcaca gtacggatac cgccgttgat ccagccccca aagggggtga
     8701 tgacgtagta catcgccagc atgacaaaaa tagcggaaag aatcggtagc aaaaatgtgg
     8761 ttttgaacgc taacaggaaa tccggtaact ggattttctg gttcatccat ttcacaaggt
     8821 aaccggcaac gatagaaata atcagcgcac caatgaaggt ggacggcacc ggcgaagagg
     8881 tcgcccattg catagtgctg ggatcgaagt tcagcagttg cgtcggctgg gtagacatca
     8941 agccgccgat aaaaccagcc ggaaacgcca gtttgccacc gatagagttc gccacaaaag
     9001 cagcaaacat cggaatggca aaaccaaaca acacgccgcc gaaggactgc gacaaccagg
     9061 caaatttcag taaggagagg tcgaaaccgg agaatttccc gctattaaga gcatccatga
     9121 tgccgatctc agcaggaatt ttaagccagc tataagcaat caactggctg aaagcgagga
     9181 tcacaccgcc cataattaag gtcggcacca tgcgtgaaat ccctgacatc acatgctgcg
     9241 ggagttcacc ccagaagctg cttttagagg cctgcggatt cttaactgcc gctgccgcac
     9301 cggatgcgcc aggcacaacg gttgcactgc gttttttaat ggccataaca attatccctg
     9361 tcgaatatta ctgttgttca gaggcaatca tctcttcgat ttctttgatg atgcccgcag
     9421 cgtttttaat tgcgtcctgc aaagtgattt cataaacgtc gcgtgattcg aaacgttcgt
     9481 tatcttccgg ggtaactgcc acggagtgga tgatgatggt cgcttcggcg atatcctgcg
     9541 ccgtcaggcg attctggata ccgtccgcgc cctgggtttc aattttcact tcataaccgg
     9601 cttctaccgc cgcttcttcc agcgcctgag cggccataaa agtgtgagcc aggcccattg
     9661 ggcaggcaca taaggcaatc agtttcttac tcatcgttaa ttcctcgttc agtgatagtt
     9721 gacgagtgaa attatgaacg tgcaaaacaa atcgccgtta ccagacaaat aatgcattta
     9781 ctggagaatt aatcccgctg tacggaagtg tgacgcaggt cggccttgtg aggggacaca
     9841 aagaaaagtt gaattaactg tatgatttaa aagattatcg ggagagttac ctcccgatat
     9901 aacaggaagg atttacagaa tgtgacctaa ggtctggcgt aaatgtgcac cggaaccgag
     9961 aagacccgga ttgtcatgga cgatgagata caccggaata tcatggacat attctttaaa
    10021 gcgcccttta tcttcaaatg cggcacggaa accagaggct ttgaagaact caaggaagcg
    10081 cggcacgata ccgcccgcaa taaacacgcc gccaaatgtc ccgagattga gcgccagatt
    10141 gccgccaaaa cggcccataa tgacgcaaaa cagcgacaac gcacggcggc aatcggtgca
    10201 gctgtcagcc agcgcgcgtt cggtaatatc ttttggcttg agattttctg gcaggcggtt
    10261 gtcggcttta acaattgcgc gatacaaatt caccagtcct gggccagaaa gcacgcgttc
    10321 cgccgaaaca tgaccaattt ccgcacgcaa tatttcgagg ataatggcct cttcttcact
    10381 attcggcgca aaatcaacgt gaccgccttc gcccggcaag cttacccaac gcttatcgac
    10441 atggaccaga tgcgcaaccc caagccccgt tccggcaccg taaaccgcaa taggcttacc
    10501 ttctaccggt tctgcgccac caaactgaat cagatgctct tttttcagca tcgggatcgc
    10561 catcgataca gcggtaaaat cgttaataat ttccagatgg ctaaaaccga gattcttttt
    10621 catttcggca attgagaacg cccaggtatg gttggtcatt gccacccagt ctccggtaat
    10681 cgggcaagcg atggcaatac agccgtcttt cacctcgacc ttatgttctt caagataaac
    10741 gcgaatgacc gcttcgaggc tggggtaatc aagccctgaa taggtcttag cctgcgagat
    10801 ttcaccactg gcaatatcac acagagcaag acgtgcgttg gtgccgccca catcaccgac
    10861 taatgcatac tttgtcattc ttcaactgct ccgctaaagt caaaataatt ctttctcaca
    10921 ctgtaaatac ctgggggcat aacaacaacg ccggaaaggc aggctccctg taaatatcga
    10981 tctgggtcac acaattactt tatcgtttca gcaccaattg cagcgatgcc tttttgcaag
    11041 ctgggcaaac taagtatctg accccgcata aggaatagaa catgctccat ccgcgagcca
    11101 gaaccatgtt gttattatcg ctccccgccg tggcaattgg gattgcgtcc agtcttattc
    11161 tgattgtggt gatgaaaatc gcctcggtat tacagaattt gctctggcaa cgactgccgg
    11221 gaactctggg gatagcccag gattcacccc tctggatcat cggtgtatta acgctaacgg
    11281 gtattgcggt ggggttggtt atccgtttca gccagggtca tgccggacca gaccccgcct
    11341 gtgaaccgct gatcggcgca ccggttccgc cctctgcgct accaggactt atcgtagcat
    11401 taattctcgg tcttgctggc ggcgtcagcc tggggccgga acatccgatc atgaccgtca
    11461 atatcgccct tgcggttgcc attggcgctc gtctgttacc gcgcgtcaac cgaatggagt
    11521 ggactatttt agcctctgcc ggaaccatcg gcgcgctgtt tggtacacct gttgcggcgg
    11581 cgttgatatt ttcgcaaacc ttaaatggca gtagtgaagt tccgctatgg gatcgtcttt
    11641 ttgcgccgtt aatggcggca gcagctggtg cacttactac cggattattt ttccatcccc
    11701 atttttcact gcccattgcg cattacggac agatggaaat gaccgatatt ctcagcggtg
    11761 caattgtcgc ggcgattgcc atcgcagcag ggatggtcgc cgtatggtgc ttaccacggt
    11821 tgcacgcgat gatgcatcaa atgaaaaatc cggtgctcgt gctgggtatt ggcggattta
    11881 ttctcggtat tctgggggtt attggtggac cagtttcgct gtttaaaggg ctggatgaga
    11941 tgcagcagat ggtggcaaat caggctttca gcaccagcga ttactttttg ctggcggtaa
    12001 ttaaacttgc cgccctggtc gttgctgccg ccagtggctt tcgcggtggg cgaatcttcc
    12061 cggcagtgtt tgtcggcgtg gcattagggt tgatgctgca tgagcacgtt cccgccgtac
    12121 cagcggcaat aaccgtttcc tgcgctattc tcggcatcgt gctggtggta acacgcgatg
    12181 gctggttaag tctttttatg gcggcagtcg ttgtacccaa taccacattg ctaccgctgc
    12241 tctgtatcgt catgcttccg gcatggctgt tattagcagg taagccgatg atgatggtca
    12301 atcgtccgaa gcaacagcca ccccacgata acgtttagca aaaaatgctc tcctttatga
    12361 tttaagagtt atggcgatga tacggggctt tacctccccg taatattgcg ttaacaggcc
    12421 gctgacggct tcgatcgtga aggagaataa caatgttcag gtcactgttt ctggcggccg
    12481 ccctgatggc atttaccccg cttgcagcaa acgcaggcga aatcacccta ctgccatcaa
    12541 tcaaattaca aattggcgat cgcgatcatt acggtaatta ctgggacggt ggtcactggc
    12601 gcgaccgtga ctactggcat cgcaattatg aatggcgtaa aaaccgctgg tggcgtcatg
    12661 ataatggcta ccatcgtggc tgggataagc gtaaagcata tgagcgtggc tatcgtgaag
    12721 gctggcgcga tcgtgacgat catcgcggaa aaggccgcgg acatggacac cgccattaaa
    12781 cacattcgcc aatggagcac aaggcctgat gcgacgctag tgcgtcttat caggcctaca
    12841 aaccggctct ttaacgcgta gggcggataa ggcgtttacg ccgcatcgcc aatagtgcca
    12901 gatgcgacgc tcattcaact acaatcccag cgccgtcccc accaataacc agatattcag
    12961 cgccacgacc agcactacaa tcacccagcc tgtctgtttt acgcgtttgc tgttcaccag
    13021 atcgcccatc aacttgctgt cactggtgaa aatcagcagt ggaaccagcg ccagggcgat
    13081 accaaaactt aacagcacct gactcataac cagaatccgt gtcggatcta atcccatcag
    13141 aatgacaata aatgacggca gcatggtgac tgtacgacgc acccacagcg ggatatggaa
    13201 gcgaatgaac ccctgcatca ccacctgccc tgccagtgtc cccaccaccg ttgaggacag
    13261 tccggcagca accagactta acccaaagac cgttgccgca gcatggctta acagcggttg
    13321 cagcgtcaaa taagcctcat caagatcggc aacaccagta tgaccagaaa agtggaacgc
    13381 cgccgcagct gtagccatca tcgccagatt gacaaaaccg gcaatagtca tggcgatagc
    13441 cacatcccat ttggtggcgg aataacgttg ttgacgcgaa ccgccatgta aatgctgagt
    13501 gagcgaagag tgcaaataaa tcacatgcgg cataatcgtc gcccctaaca cgcctgctgc
    13561 caggaagacc gcctccgaag taggtaaact cgggatcacc attcctttac ccagctgcgc
    13621 caggttaggc tgggagaaaa tcaactcgac aatgtaagcc gcggcaacaa acaacagtaa
    13681 cccgccaatc actttctcca gcggtttttg cccgcgacgt tgcagcatta aaatcaggaa
    13741 agtcgcgatc cccgtcagca ccgcgccctg caacaacgaa acaccaagaa tgagtttaaa
    13801 accgatcgcc gcaccaataa attccgccag gtcggttgcc atcgcaataa tttctgcctg
    13861 aacccaatag aaccacacta cgggacgcgg atagtgatcg cgaatctgct ccgccagatt
    13921 tttaccggtg gcaatcccta gtttggcaga gaggatctga atcagcatcg ccatcaggtt
    13981 ggcccaaacg acaacccaca gtagctgata gccgaagctg gcacccgcct gaatattggt
    14041 cgcaaagtta ccgggatcga tataaccaat cgccgcaatg aacgcaggtc ccattaatgc
    14101 gagcctcatc ttgcgcgccg cccgtccgct gctactctca acgcgatagt tcgtcatctt
    14161 gtgcctctaa aacatagcct ttgctatgtt tcatgctatg ccaaacgaga atgattatca
    14221 aattcattta aatgggttgt ggtgatttct ctgatagacc aggattatga ctacgaaaag
    14281 attgatgact tgaatgttat ggttatgttt aatgttagca catttacata actttcagct
    14341 tccatacaca acatagcaga aatgtatgac agctcactat ttttgaagct tgtcacagga
    14401 cgtcattata gtgtgtgtca gatctcgttt tccttaacca tgttacatag aatgtgcacg
    14461 gaaatttaac ctgcctcata tttggagcaa atatggaccg cgtccttcat tttgtactgg
    14521 cacttgccgt tgttgcgatc ctcgcactgc tggtaagcag cgaccgcaaa aaaattcgta
    14581 tccgttatgt tattcaactg cttgttatcg aagtgttact ggcgtggttc ttcctgaact
    14641 ccgacgttgg tttaggcttc gtgaaaggct tctccgaaat gttcgaaaaa ctgctcggat
    14701 ttgccaacga agggactaac ttcgtctttg gtagcatgaa tgatcaaggc ctggcattct
    14761 tcttcctgaa agtgctgtgc ccaatcgtct ttatctctgc actgatcggt attctccagc
    14821 acattcgcgt gttgccggtg attatccgcg caattggttt cctgctctcc aaagtcaacg
    14881 gcatgggcaa actggaatcc tttaacgccg tcagctccct gattctgggt cagtctgaaa
    14941 actttattgc ctataaagat atccttggca aaatctcccg taaccgtatg tacaccatgg
    15001 cagcaacggc gatgtccacc gtttctatgt ccatcgttgg tgcatacatg accatgctgg
    15061 aaccaaaata cgtcgttgca gcgctggtac tgaacatgtt cagcaccttt atcgtgctgt
    15121 cgctgatcaa cccttaccgt gttgatgcct gtgaagaaaa cattcagatg tccaacctgc
    15181 acgaaggtca gagcttcttc gaaatgctgg gtgaatacat tctggcaggt ttcaaagttg
    15241 ccattatcgt tgccgcaatg ctgattggct ttatcgccct gatcgccgcg ctgaacgcac
    15301 tgtttgctac cgtgactggc tggattggct acagcatctc cttccagggc atcctgggct
    15361 acatcttcta tccgattgca tgggtgatgg gtgttccttc cagtgaagca ctgcaagtgg
    15421 gcagtatcat ggcgaccaaa ctggtttcca acgagttcgt tgcgatgatg gatcttcaga
    15481 aaattgcctc tacactctct ccgcgtgctg aaggcatcat ctctgtgttc ctggtttcct
    15541 tcgctaactt ctcttcaatc gggattatcg caggtgcagt taaaggcctg aatgaagagc
    15601 aaggtaacgt ggtttctcgc ttcggtctga agctggttta cggctctacc ctggtgagtg
    15661 tgctgtctgc gtcaatcgca gcactggtgc tgtaagacca tacataaaaa agccggggat
    15721 aattccccgg cttttttacg cctgttaatc agctaatggc tgcgggcgac caatcaaata
    15781 cccttgcaga tattgcaccc cgagcttatg caatagcgcc tgttgctgtt gcgtctcgac
    15841 aaactccgcg accacactca atgacttcgc tttcgccaga tcggtaattg atctcacaat
    15901 catcgcatcc agcgtattcg tgacaatatc tttcacaaag acgccatcaa ttttgatgat
    15961 atcagcctgc aaacgcttta accgttcgta gttggcatag ccggtgccaa agtcatcaat
    16021 cgcaatccgg aaaccaaact tatgcagctg ctcgatgttg tacatgctgc tttctgcgtt
    16081 agaaaacgcc tgctcctcgg tgatctcaag aatgaccgcc tgcggggaga tgtgataacg
    16141 cttaaataga cgaataatcc gcccggcaat attcttttgc agcagcgtga gcggcattaa
    16201 attaactgaa aagcgcgggc cttttttgtc gcaagggtgt gttgccagcc acttcaacaa
    16261 ggattccagc acttgcaaat caaaacgcgc gctaaggtta aactgagcaa taaggggcag
    16321 aaacttatcc ggggtcataa tgccgccgtc atatttcagt cgcgcgagga cctcatcata
    16381 accttcaccc tctttgttgc gaattggctg ggcgtagagc agcaaatcac cctgatctaa
    16441 cgccgtgcga atggtattca gcaatagcac ctgtttagtg gtctgcccgg aaaccatctc
    16501 ctctcggcta tccagcgcca gcacatgatg atgtgcgcag gattgctccg ccagccagct
    16561 taactgcccc aacaagggtt gcagggtttc ctgatttcca tcaaaacgcc cccaggcagc
    16621 accatagccc atatccagcc cggtattgtt ccagtgaatt tgccgactat tcaggatgtt
    16681 aaccatatgc tggagtcgcc cttccgtttc aggcccgctc agcactaaca gcagttcact
    16741 acccggcaat tgatacaact tttcgttttc ctgcatcaat ggcagcagcg tacggtaaat
    16801 tgatcggata cagtgaacac gcatcatcaa cccgtaatga cgactcataa actcaagatt
    16861 atcaatgcgc aggcagcaaa aactcttgcc cgcctcttgc tccggcgctt gttccaacgc
    16921 acgaaagttg ggcaggagcg ttaacggatc ggtcagcgcc tgcaaatgcc agcggcgatt
    16981 aagccattca ctgcgatgat aaatgcgcac catatagagc aggcaaacgc taaaggaaat
    17041 cagcaccgcg agaataaatg ccagcgaata ttcggtttcc accccttgca aaaagttctg
    17101 gttgtaattc agcaggcaaa gcgttgaaac agcccaagtg agatttaaaa acggatagcg
    17161 aagcttaccg accccgaggg taaagatgat gaagaaaacg ggtaccaggt aaccggcaat
    17221 aaagtcgttt tcataaggtg tgcacaacag aagtagcagc acgcttaacg ctgccagcca
    17281 gcttaaggta aatgcgcgtt tctctttgcc caacgacgga gcgatatccc tgcgccacaa
    17341 tatctgcgca aagtggggac ttacaatcat gcgagtgaga tagtagaaaa gcatgttgta
    17401 aatcagcaca gcggtgaaaa ggcttagcaa atcaacgacc gtgaaaatgg catccgcatc
    17461 gccgaaaaag gtggatatct ttagcgggaa atcaaagaag ctccccacaa gatacatgct
    17521 gcatttgatg ccgatcggcg tcaccaaacc aagccagaac agacgttgcc agacatatcg
    17581 gctggtcaat ccataacgcc aacgcgtccc cagctgccag cgtaatattg cgcaggcaca
    17641 caacacgaca aacgtctggc taccgagcat gaccgcagtc tgtaaaaaag agagatgaaa
    17701 gttccacaga ttggtgcaaa acatcccgca taaaacggga acgacaccgc gccagccaaa
    17761 gataaacaag attgacagca ttacgcacag tggcatccac gccagaaaga tataactgga
    17821 atggataatg gcaagtggcg aaataaaacg ggatagctga ataagtacca cggtgagcgt
    17881 aaacgctagt gtgaatatct tgatattttt tatcaggtta tgctccacaa acatgagtac
    17941 actactctca ctgatcgggg ttttcaggca cacaaataat aagtttattc gcattgcgaa
    18001 gcaaacaggg gccatcctgt tgccgatttt tcatccaatg atgctttcac caggcggcac
    18061 taacccggac gtagactgta ttacaaaagc ggcaaaaagc agagacaaaa aacccccgct
    18121 ttgcagcgag ggttggaaat ttggtggagc taagcgggat cgaaccgctg acctcttgca
    18181 tgccatgcaa gcgctctccc agctgagcta tagccccacg atgcgtttac gtaccaaatt
    18241 tgctgggtgc aaaatttggt ggagctaagc gggatcgaac cgctgacctc ttgcatgcca
    18301 tgcaagcgct ctcccagctg agctatagcc ccgtcacgta aagcttgtcg agttgacggg
    18361 cggcatcata tgaattccgc ccgaatgtgt caacggcaaa ttgcaacgtg tagtttcaat
    18421 cgctgaaaaa tcaggcaaat gaacaatttt ggaaaaccgc tcgcattcag tagttattca
    18481 tgtcacggtt tcctgtaaag tggtgctata aaatgaacta ctaatagacc cacatacatt
    18541 cagggaattg ttatgttcaa ggagaggatg acaccagatg aacttgccag actgaccggc
    18601 tatagccgcc agaccattaa taaatgggta cgcaaggaag gctggacaac gtcaccaaaa
    18661 cctggcgtcc agggtggcaa agcccgactg gttcacgtca atgaacaggt tcgtgaatat
    18721 attcgtaatg ctgaacgtcc agaaggtcag ggagaagcgc ctgccctttc tggtgatgcg
    18781 ccgcttgaag tattgctggt gacactggcg aaagagatga cgccagttga acaaaaacag
    18841 tttacatcct tgcttctgcg ggaagggatt atcggattgt tacaacgctt agggattcgc
    18901 gatagcaaat aatatgaaaa gattacgcaa taaaatgacc accgaagaac tggctgaatg
    18961 cctcggtgtg gctaaacaaa ctgttaaccg ttggatcaga gaaaaaggct ggaaaacgga
    19021 aaaatttccc ggcgtgaaag gcggtcgtgc cagacttatt ctggtcgata cgcaagtttg
    19081 cgagtttatt cagaacacgc cagccttcca taacacaccg atgcttctgg aagccgaaga
    19141 accccttgcc gaatacgcac cggggattcg cactcctgct tatcggcaaa tcatcagtgc
    19201 aatagacaac atgactcaca gtgagcagga aaaagttgcg caatttttgt cacgcgaagg
    19261 aattcgtaat ttccttgctc gtctcgacat agatgaatca gcttgacatc ctccacgccc
    19321 tgaatgacga ggatccctgc tacgttcagg ctgtcgcctg aatcatttcg gtgggttcct
    19381 gcttcaacga gcggcctgac tgcaccatct ctccacaggc aagaacggcg tgccccgccg
    19441 ctaaaatgtt acgagcgccg tttacatcgg cgttcgctgt atatccacat acctggcatc
    19501 tgaatttact ttgtgacagg cggttttctt tcgctgtatg accacagcac gcgcaacgct
    19561 ggcttgtgta cgctggcgga acagccagta cctgaccgcc acgccagagc tgcttgtact
    19621 caagctggcg gcgcatttca taccagccct gatccagtat cgaacggttt aaacctgatt
    19681 ttgcccggac attgcgcccc ggctggctga ccgtacccgc tgctgacttt gacatgttgc
    19741 tgaccttcaa atcctcaatg actatcattg cgtggttttt gctgacggtc gttgtgactt
    19801 tgtgaaggta gtccctgcgg atatttgcgg tacaggaatg cagtcgctgt attttgcgtt
    19861 tctgcttctg ccagttattg ctgaatttga ccttgcggct taactgtcgc tgaagtctcg
    19921 ccaacttctt ctggtttttc tgaaaactgt ttacaggctc aaagactgtg ccatctgaca
    19981 gcgtggcgag tttagccacg ccagcatcca gtccgaccat tgatgctgaa gggtgaaccg
    20041 gagttgatac ttcactttct gtctgaatac tgatgtacca cttaccgcag gactggctga
    20101 cagtgacatt tttcacaaca cccgtgacct gacggctgtt caggtagcgc atccagccaa
    20161 gtttcggcag aaaaatacgg ctgttttcct gatcgagctt aacgccctgc gggtagcgga
    20221 atgcatcatt ctgtccccgc tttttgaatc ggggaaaagc agcccgcttc cggaagaagt
    20281 ttttgtaagc ccgctcaagc tctttcagtg actgttgcaa tggctgtgac ggggcatctt
    20341 taagccattg cgtttcagtg gcgtttttcc actcaaccag ccaggaagcc attttcccgt
    20401 aagggatgta tttattaccg gcctcatgat tctcattctg aagcgccaga gcacgattga
    20461 aaacgaaacg acatgcgcct gcgaagcgcc tcatttcacg ctcctgttga ccacctggtc
    20521 ttaactggaa tttaaatgcc tgtagtcgtt tcacaggcca aatgatactt tggtctatga
    20581 ataaagaaac cgatatccga cggggaaggc attgtgtttt cctgatgcat gttcatctgt
    20641 tctttgtcac cagataccga cgtcagattt ttgactatga cgcgacagaa aaactacgca
    20701 cttacttttc aaatgtatgt gctgattttg aagctgaact ggttgaaatg gatggcgaac
    20761 cagatcacgt ccatttgtta actaactatc ctcccaaact ggcgatatcc agtctggtaa
    20821 acagcctcaa aggcgtatcg ggtaggttac tgcgacgaga tcgaccagat attgcagtca
    20881 ggtattacta caaaggcgtt ttgtggagtc ctggctattt tgccagtagc tgcggaggtg
    20941 cgccaatatc cgtcatccgc caatacattg aacaacagca aacacctggt caggtggaaa
    21001 accgcgcctt atatccccgc cctgaaggac ggggttttac ggcgcaccgg ataaataaaa
    21061 aacggcaggg gtatctctcc tgccgtttat cttttatgcg ctaattactg ctgattttcg
    21121 cgttccgcaa taaaagccag cgctttgttg atacgctcga tactgcgggt cttaccgatc
    21181 gcgtgaacgg taacgtccag cgccggagac tgccccgcac cggttacggc gacacgcagc
    21241 ggcataccaa ctttacccat acccacttcc agctcatccg ccgtcgcctg aatagcgtga
    21301 tgaacgtttt cagctgtcca gtcagtaatc gcggccagtt tgtcacgaac cacttccagc
    21361 ggctgacgcg ctaccggacg cagatgtttt ttcgcggcgt cggcatcgaa ctcagcaaaa
    21421 tcttcgtaga aataacggca gctctgtgcc atctctttca gcgtcttgca gcgttcgccc
    21481 agcagtttca ccagatcagc cagctgcggg ccgttacggg tatcgatatt ttcctgctca
    21541 atgtgccact gtaagtgagt agcaacatac tccggcggca gcgcgttaat gtagtgatgg
    21601 ttcagccaca gcagcttgtc ggtgttgaac gcactggcag atttgctgac ggcattcaaa
    21661 gtgaagtatt tgatcatctc ttcacgagtg aagatttcct gatcgccgtg ggaccagccc
    21721 agacgcacca gatagttcag cagtgcttct ggcaaataac cgtcatcacg atactgcatt
    21781 acgctgactg ccccgtgacg tttggacagt tttttgccgt catcgccgtt gatcatagaa
    21841 acgtgcgcgt aaaccggtac cggcgctttc agggctttaa gaatgttgat ctggcgtggc
    21901 gtgttgttga tatggtcttc gccacggata acgtgggtga tttccatatc ccagtcatcg
    21961 acaaccacac agaagttata ggttggggaa ccatcggtac ggcggataat aagatcgtcc
    22021 agttcctggt tgctgaactc gatcggacca cggatctgat cgtcaaaaac aacagaacct
    22081 tcctgcgggt tagcaaaacg tacgacacac ggctcatcat cagcgtgatg ctcatgactg
    22141 tggcggcagc gaccgtcata acgcggcttc tcacctttcg ccatttgctc ttcgcgcagc
    22201 gcttccaggc gctctttaga gcaatagcat ttataagctg tgccctcttc cagcatctga
    22261 tcgatcaccg cgttgtagcg atcaaaacgt ttggtctgga agtacggacc ttcatcccac
    22321 tccaggctca gccagttcat gccatccata atggcttcga tagcttccgg cgtggaacgc
    22381 tcaagatcgg tgtcttcaat acgcagcacg aactcaccgc cgtgattacg tgcaaaaagc
    22441 caggagtaaa gagcagtacg cgcgccgcca acgtgcagat agcctgttgg gcttggcgcg
    22501 aagcgagttt tgattttcat gaaatggcct tacgtttaga aagatgccga caaccggcaa
    22561 atcctggaaa aattgagtgg gcgatattct atcactcccg cctgattcct caatgtagtt
    22621 cgggttgcaa ccttacgcat tattgttttc gttgacaaat tgcgcaacac ggtctgtttt
    22681 gcgatcatct tgcttaaatt tacgacgaac gaacaatttc tttagaaaat gcgttgactc
    22741 attttgaact ctccctataa tgcgactcca cacagcgggg gtgattagct cagctgggag
    22801 agcacctccc ttacaaggag ggggtcggcg gttcgatccc gtcatcaccc accaactact
    22861 ttatgtagtc tccgccgtgt agcaagaaat tgagaagtgg gtgattagct cagctgggag
    22921 agcacctccc ttacaaggag ggggtcggcg gttcgatccc gtcatcaccc accactttct
    22981 caccagctaa atttcttgta aaaatgtgaa gtaccgaagt gggtgattag ctcagctggg
    23041 agagcacctc ccttacaagg agggggtcgg cggttcgatc ccgtcatcac ccaccacttc
    23101 gggtcgttag ctcagttggt agagcagttg acttttaatc aattggtcgc aggttcgaat
    23161 cctgcacgac ccaccaatgt aaaaaagcgc cctaaaggcg cttttttgct atctgcgatt
    23221 tgtaagattg atttttcttc tcaagttcct tgttcttctg ccgatattac ctcgtacagt
    23281 gacgaggact gaacaatggc cactatctct tcaacttcaa ttccttccat acaaacacag
    23341 agtagtaaca gggcatcaca aggtagcgat gttgcttcac aaatcgcgcg gataagtcag
    23401 caaatcatca aactcactca acacattaaa gagattgtag acactagcgg cagtgctgaa
    23461 gacaagcaaa aacaggccga attgattcaa cagcaaatca ccctactgga aacacaactg
    23521 gcgcagttgc agagacagca ggcagaaaag gcgcaggaga aagagcagcg tttgtctctg
    23581 aatgtatccc ttctaaatcc tgttgaaaat acgactcata tcggtatcta tatctgatac
    23641 aacggatccc cttcccgccc agccattagc gtattgccct ggtctcgtac cacgtcccat
    23701 aacgcttcag cggcggtgga aagtgaacgg tttttcctac gcaccagcat caactggcgt
    23761 tccaccaccg gcgtaatgcg ctttaccacc agcgggctac cttcaggcaa tggtagcgcc
    23821 agtgcgggaa gaatactgat gccgatgcct gcggctacca tcgggaacag cgtcgccgga
    23881 tgtccaatct cctgcacaat attcgcctga ataccattgc gcgccagcgc tgcgtcaatc
    23941 agcggtcggc tgcctgacgc ataatcctgc aacaccaatt tcgctccctg taatgcctgc
    24001 cagggtacat aatcttccgc ggccaaagcg ctatcccgat ggcaaagcag aaagaaaggc
    24061 tcggaaagaa tcacttcaca ttgcagatcg cccactgggc ctggatcgat gacgatgcca
    24121 aagtcgacat ctccctggcg aatactttcc atcacccact gctgcggacg atcgtgcagg
    24181 acaaactgaa tatctggata gcggcgatgg ctttcagcaa tgcattgcgg aataagatgc
    24241 gcggaaatgg tctggctggc agcgacccga acttttccgc tcagttgttg ccccatacgc
    24301 ccggtatcgc gcaacgtgct gttaagttca tccagcagtc gctcaagacg caaagccaac
    24361 tgctgccctg catctgtaag caccacttca cgcgtggttc tgtccagcag gcgaacacca
    24421 gtatgatttt ccagctcctt cacactgtga cttactgccg actggctcag gccgatacgc
    24481 tctcctgcac gactaaaact tttctcctgc gctactgtga cgaaaacctt taattgtttt
    24541 aaagaataat tcatctgttt tcttcatgaa tggatgcaat aaatcaattt tatttctcaa
    24601 actggaagaa gcacaataga accatcgatc atctggagtc tttatgaaac tttttcgtat
    24661 cctcgatcct ttcaccttaa ccctgatcac ggtggtgttg ctggcctctt tctttcctgc
    24721 cagaggcgat ttcgtcccct tctttgaaaa tctgaccacc gcagctattg ccctgctgtt
    24781 ctttatgcac ggcgcgaagt tgtcgcgtga ggcgattatt gctggcggtg gtcactggcg
    24841 actgcatttg tgggtaatgt gcagcacctt cgtgctgttt ccgattctgg gtgtactgtt
    24901 tgcctggtgg aaaccggtaa atgtcgaccc gatgctctac tccggttttc tctacttgtg
    24961 cattctcccg gctaccgtgc agtctgcaat cgccttcacg tcaatggcgg gcggtaacgt
    25021 cgcggcggcg gtttgttctg cgtcagcatc cagtctgctg gggattttcc tttcaccatt
    25081 gctggttggt ctggtgatga atgttcacgg tgcagggggc agccttgagc aggtcggtaa
    25141 aattatgctg caactgctgc tgccgtttgt gttggggcat ctttcccggc cgtggattgg
    25201 tgactgggtg tcgcgcaata aaaaatggat tgcgaaaact gaccagacgt ccattctgtt
    25261 ggtggtttat acagcgttca gcgaagccgt cgttaatggt atctggcata aagttggctg
    25321 gggatcattg ctgtttatcg tggtggtcag ctgcgttctt ctggctatcg tgattgtagt
    25381 taacgtcttt atggcacgcc gactgggctt caataaggca gatgaaatta ctatcgtctt
    25441 ttgtggttcg aaaaagagtc tggcaaatgg catcccgatg gcaaacattc tgttccccac
    25501 atcggtgatc ggtatgatgg tgctgcccct gatgattttc catcagatcc aattgatggt
    25561 ctgtgcggtg ctggcgcgtc gatacaaacg ccagaccgaa cagttacagg cgcagcagga
    25621 aagcagcgcc gataaagctt aaagcggacg cttcaggggc tggaccagtt gcgtcagccc
    25681 ctcggtttta atcagcagcg tgatttgcat tagctcaccg agcttccctg ccgggaactc
    25741 atctttgcgg gcaaaccaca gcagatactc ctccggcagg tcgattaagc gacgcccttt
    25801 gtatttgcca aacggcatta tcgtattggc tatttcaatc agctgctctt tttccatctc
    25861 agctacccag caaacgcagc atttccgctt cgtcgatgac ttcaatgccc agttcctgcg
    25921 ccttcgccag tttagatcct gcagcttcac ccgctatcac cagatcggtt ttcttcgaca
    25981 cgctgcccgc gactttcgcg cccagttcga ccagtcgagc tttagcgtca tcacgcgaca
    26041 tctggcttaa gctgcccgta agcaccacgg ttttaccagc aaacgggctg tcaatctctt
    26101 ccgcgttgat aacgatcggc gcaggccagt gaacaccttc cgccaacagc tcgctgatga
    26161 cattgcggtt gctttcttcg gcaaagaagt tgtgaacgtg ggatgcaacg acaatgccaa
    26221 catcaggcac cttttgcagc tcttcaatcg aagcggcttc cagcgcttcc agcgttccga
    26281 aatatgccgc cagacctgct gcggtggcct cgccgacttc acggatgcca agtgcataga
    26341 ggaagcgagc aaaggtggtt tctttcgctt tttccagcgc gttaaccacg ttttgtgccg
    26401 attttggccc catacgctcc agtccggtca gtttgcctgc ggtgagtttg aacagatccg
    26461 ccggagtgtg gacatattct ttttcaacca gctgatcgat gattttgtcg cccattccgt
    26521 caacatccat cgcacggcgg gaaacaaagt gtttcagcga ctctttacgc tgcgcaccgc
    26581 aaatcagacc accagtacag cgggcaaccg cttcaccttc cacacgctca acgtcagaac
    26641 cacataccgg acaatacgtc gggaatacaa cctcacgggt atcttccggg cgttcagaaa
    26701 gcacgacgtt aaccacctgc gggatcacgt cgccagcgcg gcgaatcacc actttatcgc
    26761 caatgcgtaa accaagacgt tcgatttcat ccgcattgtg taaggttgcg ttactcacca
    26821 gcacgcctgc aacatggaca ggttccagac gcgcaacagg cgtaatcgcg ccagtacgcc
    26881 caacctgaaa ctcgacgtca cgcacaaagg tcatctgttc ctgcgccggg aatttaaacg
    26941 ctaccgccca gcgcggggct cgcgcgacaa agccaagctg ctcctgctgt tccagtgagt
    27001 tgaccttaat caccacgccg tcgatatcaa agcccagcgt cgggcggtct tcttccactt
    27061 tgtgatagaa cgccagcact tcttccgccg attcacaaag cgttacccga tcgctgaccg
    27121 gcaaccccca ctttttaaat tgcagtaaac ggccaagatg agtatccggc agctcgccac
    27181 cttccagaac accaacgcca tagcagaaaa aagtgagcgg tcgcttcgct gtaatacgcg
    27241 gatcaagctg acgcagtgaa ccagctgccg cattacgtgg gttagcaaac actttcccgc
    27301 ccgtgcgtcg cgcatcttcg ttaatctttt cgaaccccgc ctgcggcagg aacacttcac
    27361 cacgcacttc cagacgcgcc gggatattct ctccgtgcaa cttcagtgga atggcgcgaa
    27421 tagtacgcac attagacgtg atatcttccc cggtggtgcc atcgccacga gtcgcggcac
    27481 tgactaaaac gccattttca tacagaatac tgacggcaag gccatccagc ttcagctcac
    27541 agcaccaggt gactttctcg ttgcttttca gacggtcctg cacacgtttg ttgaaggcaa
    27601 gaaagctttc ttcatcaaaa acgttatcca gcgacagcat tggcacttca tggcgaatct
    27661 ggctgaaagc cgccagcggc gcagcaccta cacgttgggt aggcgaatca ggcgtaatca
    27721 gttctggatg tttggtttcc agctcgcgca gttcgcgcat cagcctgtcg tattcagcgt
    27781 cgggaatttc cggcgcatcc atcacatgat aaagatattc atgatggcga agcgtcgttc
    27841 gcagttctgt cagttgttgt tcgattgatt ccatatcgca ccatcaatgc taaaaacccc
    27901 cgacaagcgg gggttcgaag aggagttaat ttgccttaag tgtatcaggc gttggcgtct
    27961 ttaacttcgc ggatgatgtc ctggtactcg cgcaatttct gcggagtcat catacggcgc
    28021 tgatcgtcaa gcacgacacc gcccacttca tcggcaatat gctgcgcaga ttgcagcatc
    28081 agcttgaagt tctgcagctc gtcaccgtaa gacggtacct gcataaagat agtgacaccc
    28141 ggagtagtga aatccttcat ttcaggatca aaggttcccg gtttcaccat attcgccagg
    28201 ctaaataacg ccgggccgct gccatccggg ctaagatgac gatggtaaat attcatatcg
    28261 ccaaaaatga agcccgcttg ttgaatgctg ttaagaagca gttcaccgtt tagctcgcta
    28321 ccgtgatgcg ccgcgacgtt catgataatc accgcttctt tgcgcttcgg tttatccata
    28381 actggagccg gttccgctac aggctcaggc tgtggtgccg ctacgggttc tgcaggctgg
    28441 aaagcctgtt gtgccggttg cggtgctgaa tgcactggct gcggcgctgg cgcgacctgt
    28501 ggcgaaactg gctgctgcaa cggctgttca ggctgcggct gataggcagg ctgctgcacg
    28561 ggttgctgca ccggctgcgc tggacgcgga gcatgttgcg gcggtacctg cgcttcaggc
    28621 ggctgctgga ccggttgacg cggctgcgca gacgcataag gcggttggta ctggtgttgc
    28681 ggcgacggac gagcagcctc atgctcctgg gcgttagccg gggcatgatt cacgcggtga
    28741 acacgaacct caccaacgcc ctcatcatct tcgacatcct cgtcataaga atcgtcgtca
    28801 cgttttgact tcattcgttt taatggccga tcgcggaaca tagaagatcg ttctttacgg
    28861 ctggtccaga aaccatgtac cagtaaagcg attatggcga tcgcgccaac aatgattaat
    28921 atcagacgca aatcctgcat cattatattc tctgttgttc taacaccttg ccaccacggc
    28981 aaacatttac tcactaagag tatttgccga ttacctcaag tgcaagtgca ctattaactt
    29041 tcacagcaca aagatagatg aaatcgtgct ttttgctgtt ttttcgaaca tatcctaact
    29101 gtccattgcg caattacccg gtaaaatacg cacaattttc ctgggattgg tcaaaaggag
    29161 ctcatcctga ttatggtttc atcattcaca tctgccccac gcagcggttt ttactatttt
    29221 gcgcaaggct ggaagctcgt ctcgcaacct gggattcggc gtttcgttat tttaccgctg
    29281 ctggtcaata ttttgttgat ggggggcgca ttctggtggc tctttacgca actcgatgtc
    29341 tggatcccga ctctcatgag ttatgttccg gactggttac aatggctgag ctatctgttg
    29401 tggcctctgg ctgtcatctc tgtgctgtta gtgtttggct atttcttctc cacgattgct
    29461 aactggattg ccgctccgtt taacggttta ttggctgaac aactggaagc acgattgact
    29521 ggcgctacac cgccagatac cgggattttc ggtatcatga aagatgtgcc gcgaatcatg
    29581 aaacgcgaat ggcaaaaatt tgcctggtat ctgccacgcg caattgtatt gctaattctt
    29641 tacttcatcc ctggtattgg gcaaaccgtc gcgccggtac tgtggttcct gtttagcgcc
    29701 tggatgttag ccatccagta ttgcgattac cccttcgata accacaaagt gccgtttaaa
    29761 gagatgcgca ccgccctgcg cacacgcaaa atcaccaata tgcagtttgg tgctttaacc
    29821 agtctgttta cgatgatccc gctgcttaat ctgttcatta tgcccgttgc cgtttgtggc
    29881 gcgacggcga tgtgggtcga ttgctatcgc gataaacacg cgatgtggcg gtaacaatct
    29941 accggttatt ttgtaaaccg tttgtgtgaa acaggggggg cttatgccgc cccttattcc
    30001 atcttgcatg tcattatttc ccttctgcat atagatatgc taaatcctta cttccgcata
    30061 ttctctgagc gggtatgcta cctgttgtat cccaatttca tacagttaag gacaggccat
    30121 gagtaagatt tttgaagata actcgctgac tatcggtcac acgccgctgg ttcgcctgaa
    30181 tcgcatcggt aacggacgca ttctggcgaa ggtggaatct cgtaacccca gcttcagcgt
    30241 taagtgccgt atcggtgcca acatgatttg ggatgccgaa aagcgcggcg tgctgaaacc
    30301 aggcgttgaa ctggttgaac caaccagcgg taataccggg attgcactgg cctatgtagc
    30361 tgccgctcgc ggttacaaac tcaccctgac catgccagaa accatgagta ttgaacgccg
    30421 caagctgctg aaagcgttag gtgcaaacct ggtgctgacc gaaggtgcta aaggcatgaa
    30481 aggcgcaatc caaaaagcag aagaaattgt cgccagcaat ccagagaaat acctgctgct
    30541 gcaacaattc agcaatccgg caaaccctga aattcacgaa aagaccaccg gtccggagat
    30601 ctgggaagat accgacggtc aggttgatgt atttattgct ggcgttggga ctggcggtac
    30661 gctgactggc gtcagccgct acattaaagg caccaaaggt aagaccgatc ttatctctgt
    30721 cgccgttgag ccaaccgatt ctccagttat cgcccaggcg ctggcaggtg aagagattaa
    30781 acctggcccg cataaaattc agggtattgg cgctggtttt atcccggcta acctcgatct
    30841 caagctggtc gataaagtca ttggcatcac caatgaagaa gcgatttcta ccgcgcgtcg
    30901 tctgatggaa gaagaaggta ttcttgcagg tatctcttct ggagcagctg ttgctgcggc
    30961 gttgaaacta caagaagatg aaagctttac caacaagaat attgtggtta ttctaccgtc
    31021 atcgggtgag cgttatttaa gcaccgcatt gtttgccgat ctcttcactg agaaagaatt
    31081 gcaacagtaa tgccagcttg ttaaaaatgc gtaaaaaagc accttttcag gtgctttttt
    31141 gtggcctgct tcaaactttc gcccctcctg gcattgattc agcctgtcgg aactggtatt
    31201 taaccagact aattattttg atgcgcgaaa ttaatcgtta caggaaaagc caaagctgaa
    31261 tcgattttat gatttggttc aattcttcct ttagcggcat aatgtttaat gacgtacgaa
    31321 acgtcagcgg tcaacacccg ccagcaatgg actgtattgc gctcttcgtg cgtcgcgtct
    31381 gttaaaaact ggcgctaaca atacaggcta aagtcgaacc gccaggctag actttagttc
    31441 cacaacacta aacctataag ttggggaaat acaatgttcc agcaagaagt taccattacc
    31501 gctccgaacg gtctgcacac ccgccctgct gcccagtttg taaaagaagc taagggcttc
    31561 acttctgaaa ttactgtgac ttccaacggc aaaagcgcca gcgcgaaaag cctgtttaaa
    31621 ctgcagactc tgggcctgac tcaaggtaca gttgtgacta tctccgcaga aggcgaagac
    31681 gagcagaaag cggttgaaca tctggttaaa ctgatggcgg aactcgagta atttcccggg
    31741 ttcttttaaa aatcagtcac aagtaaggta gggttatgat ttcaggcatt ttagcatccc
    31801 cgggtatcgc tttcggtaaa gctctgcttc tgaaagaaga cgaaattgtc attgaccgga
    31861 aaaaaatttc tgccgaccag gttgatcagg aagttgaacg ttttctgagc ggtcgtgcca
    31921 aggcatcagc ccagctggaa acgatcaaaa cgaaagctgg tgaaacgttc ggtgaagaaa
    31981 aagaagccat ctttgaaggg catattatgc tgctcgaaga tgaggagctg gagcaggaaa
    32041 tcatagccct gattaaagat aaacacatga cagctgacgc agctgctcat gaagttatcg
    32101 aaggtcaggc ttctgccctg gaagagctgg atgatgaata cctgaaagaa cgtgcggctg
    32161 acgtacgtga tatcggtaag cgcctgctgc gcaacatcct gggcctgaag attatcgacc
    32221 tgagcgccat tcaggatgaa gtcattctgg ttgccgctga cctgacaccg tccgaaaccg
    32281 cacagctgaa cctgaagaag gtgctgggtt tcatcaccga cgcgggtggc cgtacttccc
    32341 acacctctat catggcgcgt tctctggaac tgcctgctat cgtgggtacc ggtagcgtca
    32401 cctctcaggt gaaaaatgac gactatctga ttctggatgc cgtaaataat caggtttacg
    32461 tcaatccaac caacgaagtt attgataaaa tgcgcgctgt tcaggagcaa gtggcttctg
    32521 aaaaagcaga gcttgctaaa ctgaaagatc tgccagctat tacgctggac ggtcaccaag
    32581 tagaagtatg cgctaacatt ggtacggttc gtgacgttga aggtgcagag cgtaacggcg
    32641 ctgaaggcgt tggtctgtat cgtactgagt tcctgttcat ggaccgcgac gcgctgccca
    32701 ctgaagaaga acagtttgct gcttacaaag cagtggctga agcgtgtggc tcgcaggcgg
    32761 ttatcgttcg taccatggac atcggcggcg acaaagagct gccatacatg aacttcccga
    32821 aagaagagaa cccgttcctc ggctggcgcg ctatccgtat cgcaatggat cgtaaagaga
    32881 tcctgcgcga tcagctccgc gctatcctgc gtgcctcggc tttcggtaaa ttgcgcatta
    32941 tgttcccgat gatcatctct gttgaagaag tgcgtgcact gcgcaaagag atcgaaatct
    33001 acaaacagga actgcgcgac gaaggtaaag cgtttgacga gtcaattgaa atcggcgtaa
    33061 tggtggaaac accggctgcc gcaacaattg cacgtcattt agccaaagaa gttgatttct
    33121 ttagtatcgg caccaatgat ttaacgcagt acactctggc agttgaccgt ggtaatgata
    33181 tgatttcaca cctttaccag ccaatgtcac cgtccgtgct gaacttgatc aagcaagtta
    33241 ttgatgcttc tcatgctgaa ggcaaatgga ctggcatgtg tggtgagctt gctggcgatg
    33301 aacgtgctac acttctgttg ctggggatgg gtctggacga attctctatg agcgccattt
    33361 ctatcccgcg cattaagaag attatccgta acacgaactt cgaagatgcg aaggtgttag
    33421 cagagcaggc tcttgctcaa ccgacaacgg acgagttaat gacgctggtt aacaagttca
    33481 ttgaagaaaa aacaatctgc taatccacga gatgcggccc aatttactgc ttaggagaag
    33541 atcatgggtt tgttcgataa actgaaatct ctggtttccg acgacaagaa ggataccgga
    33601 actattgaga tcattgctcc gctctctggc gagatcgtca atatcgaaga cgtgccggat
    33661 gtcgtttttg cggaaaaaat cgttggtgat ggtattgcta tcaaaccaac gggtaacaaa
    33721 atggtcgcgc ctgtagacgg caccattggt aaaatctttg aaaccaacca cgcattctct
    33781 atcgaatctg atagcggcgt tgaactgttc gtccacttcg gtatcgacac cgttgaactg
    33841 aaaggcgaag gcttcaagcg tattgctgaa gaaggtcagc gcgtgaaagt tggcgatact
    33901 gtcattgaat ttgatctgcc gctgctggaa gagaaagcca agtctaccct gactccggtt
    33961 gttatctcca acatggacga aatcaaagaa ctgatcaaac tgtccggtag cgtgactgtg
    34021 ggtgaaaccc cggttatccg catcaagaag taattcttgc tgcagtgaaa aatggcgccc
    34081 atcggcgcca tttttttatg cttccgccag cggcggcaaa atcaattcat cgctctcatg
    34141 ctgctgggtg tagcgcatca cttccagtac gcgcaacccc gcgcggtgca ccgcatcgtt
    34201 taatgccttc cctttcagca aaccactgat aagctgagca caaaacaggt cgccagtccc
    34261 tttcaggtcg gtttttaccc ttgaatggga aatgacattc acgctgtcgg cagtgaccac
    34321 cacaaccagc atctcctgat tttcttcatt accggaggcg ctggtaatca ccacccattt
    34381 tagtgtgtct gaaagcagac tttttgcggc agcaatggca ctgtcgagat cgcggcaatt
    34441 tttaccggtc aggatttcca actcaaagat attgggggta attccctgcg ccagcggcag
    34501 taaatattgt cgatacgctt cgggaaggtc aggtttgaca taaattccgc tgtcaatatc
    34561 gccaatcacc ggatcgacca tgatcaatag gtcaggatgg tctttgcgta gcgcagtcag
    34621 ccactcggca aggattttga tttgcgatgc cgttcccata tagcccgtgg ttacagcacg
    34681 aagttggcgc agcgcatcac gctcctgaag tgcacgcaaa tagccgctaa accattcatc
    34741 cggaatcgca ccaccgtaga aagtgtcata atgcggcgta ttgctcagca ataccgtcgg
    34801 cacggcaaag acattcaggc cgttctgttt gatagcaggc acggcaatgc tgttgcccac
    34861 gctgccgtaa accacctgcg actgcacggc gacgatatcc gcctgcagcg ccctgctctt
    34921 atcgttaaac aacaacaaac tactcattta attttttctc cttgccgatg atcctcatcg
    34981 tattccaacc gaaactttac ctgattctgg cagtcaaatc ggctattaca aaacaaggat
    35041 aaggtaattc agtgaagaaa atcatttgtc tggtcattac actactaatg acactccccg
    35101 cttacgcgaa gttaactgcc catgaagaag cccgcatcaa cgccatgctg gagggattag
    35161 cacagaaaaa ggatttgata tttgtgcgca acggtgatga acatacctgc gatgaagcgg
    35221 tttctcatct gcgtctgaag ctgggcaata cccgtaaccg cattgacact gccgagcagt
    35281 ttattgataa ggttgcttcg tcgtcatcga ttaccgggaa gccgtatatt gtgaagatcc
    35341 ccggtaagag cgatgagaat gcacagcctt tttacatgcg ttaattgcgc agacggataa
    35401 aacggtgcct gcgcaataat cttaaatccc cgccccctga ctaaaatgct cttccccaaa
    35461 caccccggtg gaaaggtagc gatcgccacg atcgcagatg atcgctacca ccaccgcacc
    35521 agggttagct tttgccaccc gcagtgctcc ggcaaccgcg ccgccggagc tgacgccaca
    35581 gaatattcct tcccgcaccg ccagttcgcg catggtgttt tccgcatcgc gctgatgaat
    35641 atccagcacc tcatctacca gagaagcgtt gaaaatcccc ggcagatatt ccgcaggcca
    35701 gcggcgaatg ccgggaatgc tactgccctc ttccggctgc aggccaacaa tggtcaccgg
    35761 tttggattgt tcgcgcataa agcgtgaaac gccggtgata gtgccggttg ttcccatgct
    35821 ggagacaaaa taggtaatgc gcccgccagt ctgctgccag atttccggcc cggtagtggt
    35881 gtaatgggcg taagggttat cgggattatt gaactgatcg agcagctttc cttcaccacg
    35941 atccgccatc tccagcgcca gatcgcgcgc accttccatg ccctgctctt tggtgaccag
    36001 aatcagttcc gcaccataag cacgcatcgc cgcacggcgt tcctggctca tgttgtcggg
    36061 catcagcaat ttcatgcgat agcctttaag cgcggcaatc attgccagcg caatgccggt
    36121 gttaccactg gtggcttcga ttaacacatc acccggttta atttccccgc gcttttccgc
    36181 ctcgacgatc atcgaaagcg ccgcacgatc ttttaccgaa cctgccgggt tattgccttc
    36241 cagttttaac cacacttcac tgccgttatt cggccccatt cgctgcaact tcaccagagg
    36301 cgtattgcct attgtttgtt ctaatgtact cacgatctct atccatacgt ggtgttgcct
    36361 gatgcgacgc ttgcgcgtct tatcaggcct acaggttaca aaccttgcca ttaaaaagcc
    36421 cggatcgcgg gaagcgcctc cgggcgttta acattcactc aacctatcag gcgctttgtg
    36481 cgagagcaag ttcctcatct cggggttcga tacgctcgtc gccgttatac agccgcgcat
    36541 gttgcagacc aacgaataaa cgctcgccac gctgcggggc atcgtcgcca tgcatcacga
    36601 ccgtcagcgg ttcgttgtac caccccagcg gctgcaccac taattgggtg tagtgacctt
    36661 tcgggctggc ttccagtacc tgtaccggca gcggcgaatc gaggctggta cggcggctga
    36721 tatccacttc ccacgggcgc aggaagagat ccaccggccc ctgatacgca ggtgtgtagc
    36781 ccagcggcca gcgatgtgcg ccaacatgga actgcccgcc gcgaatggtt ccctgcaggc
    36841 ggttcacttc gcccataaat tcgagcacaa aacgggtcgc cggttcgcgc catacctgat
    36901 ccggcgcgtc agcctgttca atattgccct ggctcatcac aactacacga tcagctactt
    36961 cggtcgcttc ttcctgatcg tgggtcacaa aaacgctggt gaattttagt tcttcatgga
    37021 gttgacgcag ccatcgacgc agctctttac gcacctgcgc atccagcgcg ccaaacggtt
    37081 catcaagcag cagaatttgc ggttctacag caagcgcacg cgccagcgct acacgctgtt
    37141 tctggccgcc ggaaagctgc gccggataac gatccgccag atgggcaagc tggaccattt
    37201 ccagcaattt tgtcactttc gctttgatgg ctgcggcatt cgggcgctcg cgacgcggca
    37261 gcaccgtcag gccaaaagcg atattgtcga acaccgtcat atggcggaac agcgcgtaat
    37321 gctggaacac gaaaccgact ttacgatcac gtgcgtgcag gcggctcacg tcggtgccgt
    37381 ggaagcgaat atgcccgctg gtttgatgct ccagcccagc gataatgcgc agcagcgtgg
    37441 ttttcccgga accggacggc cccagcaacg cgaccatctg acctgaagga atatccagtg
    37501 agatatcgtt cagcacctgg gtgcgaccaa acgacttctt aatattggca atctcaatgc
    37561 tcatgatttt cctcctgctg cgcgcgtttt tcctgattct ccaggcgcca ctgcaacata
    37621 ctctttaaaa acagggtgat aatcgccatc agcgtcaaca gcgcggcggc ggtaaaggag
    37681 ccgactgtgt tgtagtcctg ctccagcaat tcaatctgta acggcagcga aagtgtttcg
    37741 ccacgaatcg agccggaaac caccgacacc gcgccaaact cgccaatcgc gcgggcgttg
    37801 gtcagcacta cgccataaag cagcgcccag cggatgttcg gcaatgtgac acggcggaac
    37861 atctgccagc cggatgcgcc aagcaaaatc gccgcttcgt cttcctgact gccctggctt
    37921 aacatcaccg gcaccagttc gcgcaccaca aacggacacg tcacgaagat cgtgaccagc
    37981 accattccgg gccaggagaa cataatttgc agattatgtt catccagcca gccgccgagc
    38041 gggccgttgg agccgtagaa cagcagatac accagaccgg caaccaccgg tgacacggca
    38101 aaaggaatgt ccagtagcgt cagcagtaac tggcgtccgg ggaagttaaa gcgcgtcacc
    38161 agccaggcca gcagaatacc gaacaccagg tttaccggta cggcaatcag cgcgatcatc
    38221 accgtcagcc agatggcgtg cagcatatcc ggatcggcca gattctgtaa aaccggcatc
    38281 agccctttgc tgaacgcctg tacgaagata taaatcatcg gcaccagcag gatgaacgcc
    38341 gataccagca tcccgatgcc aatcagaaac catttgcccc agttaatcgg gcgcgcgtca
    38401 taacgcttca attgtgtcac ttccgccatt aatgacctac cacacgccga ccaaagcgac
    38461 tttgcagagt gttaattgag aacagcagca gcagagatgc cgcgaggatc accgaagcaa
    38521 tcgcgctcgc cgccgggtaa tcaaactcct gtaagcgaac gaaaatcatc agcgacgtca
    38581 cttccgtctt ccaagcgatg ttcccggcga taaatatcac cgcgccaaac tcaccaagac
    38641 tacgggtaaa cgacaacgcc acgcccgcca ccagcgccgg agaaagttcc ggcagcacca
    38701 ctttgcagaa actctgccag cgcgttgcgc caagcgtttc tgccgcttct tcatattccg
    38761 ggcctaactc ttccagcacc ggctgcacgg tacgtaccac aaacggaatg ctggtaaagg
    38821 ccattgccac cgcaatcccc agccaggtat aggtgacttt gatatcaaac ttcgccagcc
    38881 attcaccgta aaaaccgttt acagaaaaga gcgaggccag cgttaaaccg gcgactgccg
    38941 ttggcagcgc aaagggtaaa tccatcagcg catcaagcag cgtgcggccc ggaaagcgat
    39001 aacgggttaa aatccacgcc atcagcaggc cgaaaacgcc gttaaaaatt gatgccacaa
    39061 acgccgacag cagcgtcact ttgtaggccg cgaccacctg cgggttggtg atcacttccc
    39121 agtactgcgc ccagctcatc tcagaaagct gcatcaccag cgcggagagt ggcagcagca
    39181 atatcaggca cacaaacagc agactggtgc cgaggcttaa ggtaaagccc ggcaggacgc
    39241 gtcttgatga aactgcaaac attaattacg ccccgccgct aacagcttgt ctaactcgcc
    39301 gccgctggta aagtgggttt tcatcacttc cggccaggag ccaaatttgt cttccacgcg
    39361 gaacagttcg gtctgcggga atttatcttt cagtttgtcc atgacttccg ggttattcac
    39421 gcggtagtaa tagtcggtga tgatggtttg cgcctgcggg ctgtaaagcc agttcagata
    39481 ggcttttgct gctttttcgg taccgttggc ttgcacgttt ttatcgaccc acgccaccgg
    39541 gaattctgcc agaatgttgg ttttcggaat caccacttca aagccctgcg cttcgtactg
    39601 tttgcggatg ttgttcactt ccgattcaaa gctaatcagt acatcgccca ggccgcgctc
    39661 ggcaaaggtg gtggtcgcgc cacgaccgcc ggtatcgaac acttcaacgt ttttcaggaa
    39721 ctgagtcata aactgttcgg ttttggcttt gtcgccaccg tcagctttat ccgctgcgcc
    39781 ccatgccgcc agataggtat aacgcgcgtt acccgacgtt ttcgggttcg ggaaaatcag
    39841 cttcacgtcg gagcgcacca ggtcgttcca gtcgtggata ttcttcgggt tgcccttacg
    39901 gaccaggaag cccatggtgg agtagaacgg cgagctgtta ttcggcaggc gcgactgcca
    39961 gtccgccggg ataagcttgc ctttatcgtg cagaatttgt acgtcggtca cctggttata
    40021 agtgacaacg tcggctttta agccctgcaa aattgccagc gcctgttttg atgacccggc
    40081 atgagattgt tttatcgtca gtttgtcgcc gccgttatct ttggcccatt gctgttcaaa
    40141 cggcgggttc agggcggcaa acagctcgcg ggagacgtca taagaactgt tcagcagttc
    40201 cgttgcctgt acatggcccg ccagcagcag agaagcgacc agcgcgagtg agttcttttt
    40261 cagtaagtta acggccattg cgcaccctta taaatttaat gactttctaa tagccatcat
    40321 atttataacg gacgtgaaag gagtaacggt tttatatacc gtttggtgat ttggaagttg
    40381 aaaagggaat aagacgaaat cctccccaca aatgcaggga ggaaacagag gtgaatcaga
    40441 taccgacgct aaccgtctcc ggcagtgtgc tgccgccatc aatcacattc tgtgtaccgg
    40501 ttaaatagct ggattcatcc gatgcgagga aggccgccag ttcgccgact tccagcggat
    40561 cggcgaggcg acgcagcggg attgcttttg ccatttcagt cagcaccgac tctggatctt
    40621 ccgggttcga ctggcgggca atgctttccg ccattggtgt gcgaacgtat ccagggcata
    40681 tggcgttaac gcgaatacca gactgcgcat actccaccgc cagcgatttt gtcaggccaa
    40741 caatcgccgc tttcgttaag gcgtacgccg tttcacccgg atcggccacc atatcaccag
    40801 tgactgaaga catcatcaca atgcgaccat ctttgcgggc aatcatctcc ggcagcaccg
    40861 ccttcgtgac gttccatacg cctttaatat tgatgtcaat atggaaatcg cgatcgtcat
    40921 cgctcatatc gaggaaactg cccagacgac aaacgcctgc gttattcacc aggatatcaa
    40981 tgcgcccttc tttttccttc gcgcgtttga tagctgcggc taccgacgcc gggtcacgca
    41041 catcggcgac aaccgccgta cagcgatgac cacgaccaca cagttcgtcc gccagctttt
    41101 cgatctcagg ggagatatcc agcaagatta ggtttgcgcc atgacgcgca aaagttctgg
    41161 caattccttc gccaattccc tgcaatgcgc ccgtaatcag tgctgtcttg cccgtgagtt
    41221 tacccatttt aatgctctcc ttgttaatac agagcgttac actccattcc ctctgaaaat
    41281 acaggataaa caaaatatta ctgcggatca gctcactaat tcaggaaaat ctcaatccag
    41341 tcgctgcttt atgcatcatt tcaggcgttg tgttagctca ctgctgcgct gaatcatttt
    41401 caatgactcc acatcattga gttgcacaag accaacaaac agtaaatcgg taacagaatt
    41461 ttgcgccgtg cgggtagaca tcgacgagct acgccattcg gtttcaccgg aaacggtatc
    41521 gagcgtgaaa tgcgccagcc gccgcagcgg agagtcagcc agagaagtaa tggcaatcac
    41581 cgttgccccc tgctttcgtg ccgcttctgc gcacaagaca atctcttttt tactgccgct
    41641 gtaagagata gcgatttgta catcgccttt tttcaacgcc tgggaaacgg tggcttgtac
    41701 gtgcgtgtcg gcttcgcagg ccacgcgata tccaattttc atcagtttga acgacagatc
    41761 gcaccccacc agcgccgatc cgcccaggcc cgttatctgg ataaacggtg ctttactgat
    41821 gacctcaatg attttttgca gccgcgcgta gtcgaacaac gcacaggttt gctccagcgc
    41881 cagctctttt tcgcgattca atttgcgggc aatcacttcc agcgaatcat cactggtaat
    41941 cgaactgtgc aggtgctgcg ccgtggcatt cgttttctcc cggctggcac tgtattcgcc
    42001 tattaacgcc atacgcaatt cagtaaaccc ttgtgcgccg agcttctggg caaacttcac
    42061 aatactcgac tggctgatgc cgagctgttt cgccatcttg cgcgaagaga cggatttcag
    42121 ctcactcacg ttggcccgca gaaagtcagc aattttttgc tcgttctcgg taaattcact
    42181 ttccgcattg cgtattttcg tcaggtacaa catcttccac tctcctgggc tggcagcaaa
    42241 aattccgctt aagttcttat gacgctcttc acactctgcg agtgtagact caatgattcc
    42301 tttaagtctg tcaattcggg atatatgatt ccatacatct gcaattattt gatcgtaaat
    42361 agtaaggtca ccaccgatgc aacttgaaaa gatgattacc gaaggctcga acgccgcctc
    42421 ggctgaaatt gaccgcgtat caacgctgga aatgtgccgg attatcaacg atgaagataa
    42481 aaccgtaccg cttgccgttg agcgcgtact gccggatatc gccgcggcaa tcgatgttat
    42541 ccacgcccag gtaagcggcg gcggtcgtct gatttatctc ggtgcgggaa catccggtcg
    42601 tctggggatt ctggatgcca gcgaatgtcc gcccacctac ggcgtgaaac cgggtctggt
    42661 agttggatta attgctggcg gcgaatatgc cattcagcac gcggtggaag gcgctgaaga
    42721 tagccgggaa ggcggtgtta atgatttgaa aaacatcgga ttaaccgcgc aggatgtggt
    42781 ggttggcatt gctgccagcg gtcgcacgcc gtatgtgatt gccggactgg aatatgcacg
    42841 ccagctcggc tgccgtacag tgggaatttc ctgtaatccg gggagcgccg tttccaccac
    42901 cgctgagttt gctattacgc cggtggttgg ggctgaagtg gtcaccggtt cttcgcggat
    42961 gaaagcaggc acggcgcaga aactggtgct caatatgctt tccaccgggc tgatgattaa
    43021 atccggcaaa gtgttcggca acctgatggt cgatgtggtc gccaccaacg aaaaactgca
    43081 tgtgcgccag gtcaatatcg ttaaaaacgc caccggatgt aatgccgaac aagcagaagc
    43141 agcgttaatt gcctgcgagc gcaactgtaa aacggccatt gtgatggtgc tgaaaaatct
    43201 cgatgccgca gaagctaaaa aacgcctgga tcaacacggc ggctttattc gtcaggtttt
    43261 agacaaggaa taacccatgg ccaaagagat cagcagtgaa cttctgaaca ccattcttac
    43321 ccgtgtcggc ggaccgggaa atatcgccag ttgtggtaac tgtatgacgc gcctgcgtct
    43381 gggtgtacat gacagttcac tggttgatcc cgatatcaaa acgctggaag gcgtgaaggg
    43441 cgtcattttg accagtgacc aggtgcaggt cgtttttggg cctggtaaag cgcatcgtgc
    43501 cgcaaaagcg atgagcgagc tgctgggaga agcaccggta caggatgccg cagaaatcgc
    43561 cgcccagaac aaacgtcagt taaaagccaa acaaacctcc ggcgtgcaac aatttctcgc
    43621 caaattcgcc accatcttca cgccgcttat ccccggtttt attgccgccg gtctgctgct
    43681 ggggatagcg acgttaattg ccacggtgat gcacgttccg gcagatgctc agggcacgct
    43741 acccgacgcg ctgaatttta tgaaggtgtt cagcaaaggt ttgttcacct tcctggtgat
    43801 tctggtgggc tataacgccg cccaggcatt tggcggcacg ggcgtcaatg gcgcaattat
    43861 tgccgcactc tttttgctcg gttataaccc cgctgcaacc accggttact acgccggttt
    43921 tcacgatttc tttggtctgc ccatcgatcc gcgcggcaat attatcggcg tgttgattgc
    43981 cgcgtgggcc tgcgcccgca tcgaaggcat ggtgcgccgc tttatgcccg acgatctcga
    44041 catgctgctg acctcgttaa tcaccctact gatcaccgct acgctggcct atctgatcat
    44101 tatgccgctg ggcggctggt tattcgaagg tatgtcgtgg ctgtttatgc acctgaacag
    44161 taatccgctc ggttgtgcgg ttttagccgg gctgttcctg atcgccgtgg tatttggcgt
    44221 gcatcagggc tttattcctg tttacctcgc gttaatggac agccaaggat tcaacagctt
    44281 atttcccatc ctttcgatgg caggcgcggg ccaggtgggc gcggcgctgg cactctactg
    44341 gcgggcgcaa ccgcacagtg cattacgcag tcaggtacgc ggggcgatta ttcccggcct
    44401 gctgggcgtt ggcgaaccgt tgatttatgg cgtcaccctg ccccgcatga aaccgtttat
    44461 taccgcctgt ttaggcggcg cggcgggcgg tttgtttatc ggattgatag cctggtgggg
    44521 tctgccgatg ggcttaaaca gcgcctttgg gccgtctggt ctggtggcgc tgccgctgat
    44581 gacttcagca caaggcattc ttccggcgat ggcagtctac gctggcggga ttctggtggc
    44641 atgggtttgc gggtttattt tcaccacgct ttttggctgc cgtaacgtca atctggactg
    44701 atatcatgaa acggacaatg ctctatcttt ctctgctggc tgttagttgt agcgtcagcg
    44761 ccgcaaaata ccctgttctg acagaaagtt cgccagagaa agcagggttc aacgtcgaac
    44821 ggcttaacca gatggatcgc tggattagcc agcaagtgga cgccggttat cccggcgtaa
    44881 acctgctgat tataaaagat aatcagattg tgtatcgcaa agcctgggga gcggcgaaaa
    44941 agtacgatgg cagcgtattg atggcgcagc ctgtaaaagc taccaccggg acgctgtatg
    45001 acctggcctc aaacaccaaa atgtatgcca ctaacttcgc tctgcaaaag ctaatgagcg
    45061 aaggcaaact gcatcctgac gatctgattg cgaaatatat ttcggggttt gccgatagtc
    45121 caaatgacac catcaaaggc aaaaacacgc tgcggatttc tgacctgctg catcacagcg
    45181 gcggtttccc ggcggaccca caatatccga ataaagcggt cgcgggcgcg ttatattccc
    45241 aggataaagg ccagacgctg gagatgatca agcgcacgcc gctagagtat cagcccggca
    45301 gcaagcatat ttacagtgat gtcgattata tgctgcttgg atttatcgtc gagtcggtca
    45361 ccggtcagcc gcttgaccgc tatgttgaag agtcgattta tcgcccgctc ggcctgacac
    45421 atacggtatt taacccgctg ctgaaaggct ttaaaccgca acaaattgcc gccacggaat
    45481 tgaacggcaa tacccgcgat ggagtgatcc acttcccgaa tatccgcacc tccactcact
    45541 ggggtcaggt gcacgatgaa aaagcctttt attcgatggg cggcgtttcc gggcacgcag
    45601 gtttgttttc caataccggc gatattgcgg tgttaatgca aacgatgctg aacggcgggg
    45661 gctatggcga tgtgcagctg ttcagtgcgg aaacggtgaa gatgttcacc accagctcta
    45721 aggaagatgc cacttttggc ctcggctggc gcgtgaatgg taatgccacc atgacgccga
    45781 cgtttggcac gctggcaagc ccgcaaacct acggtcacac tggctggacc ggaacggtga
    45841 ccgttatcga tccggtgaat catatggcga ttgtgatgtt aagcaacaag ccacattcgc
    45901 cggttgccga tccgcaaaag aatcccaata tgttcgaaag cggtcagctg ccgattgcaa
    45961 cgtatggttg ggtagttgat caggtgtatg cggcgttaaa gcaaaagtaa taaaaaggcc
    46021 agtcagtaat gttactgcct ggcctttaat caatgaatca gaaacgctta cagcgccatc
    46081 aacttgtcca gcgacggtgc gaaataatag ccgccggtta ccggtttggt gaaacgcaac
    46141 atcgcatcac gcttaccatc ggtatcgcca aacatgctca gcagttgctg ctcaatgtta
    46201 tgcagacgcg cgcagtaggc gcagaagtac agaccgtgag tgccactggc ggtgccgtac
    46261 ggcaggctct ggcgaacaat cttcagccct ttgccatctt ctttcagatc aacgcgggtc
    46321 aggtgagagg tttccggacg ttcgtcgccg tcaatctctt cgttggcctc tttggtgcgc
    46381 ccgatcatca tctcctgatc gtgaacgctc atccggttga gctgcttcag gttgtgctcc
    46441 caacgctgca caaacacata gctgccgccg gcatccacgc cgtctttgat aaccgccact
    46501 tcgcgacgcg tctcttcacc cgccgggttt tctgtgccgt caacaaagcc gctcaggtca
    46561 cgctcttcca cccaacggaa gccgtggatc tcttctttca cttcaatgca gtcaccaaag
    46621 gcttccatcg ccgcctgggc gacagagaag tttacgtcgt gacgcagaga aagaatgtgg
    46681 atcaacacat cgaactgggt ggtcggcgca aggcctttac cgtagcccgg aaaatctttc
    46741 agctcttctg ccccaacgcc gccgctcaga gcgcgccaga tgttgttacc aaaggcaacc
    46801 accgcaccaa gatgcgcgtc cgggaatttc gcttcaaaag ttgccagttt gtcggcaaat
    46861 gttttactgg ccgcacgcag ggcgtcaact tcccctttca cgttggcttc gatccaaatc
    46921 gccgcgcggc aatgttctgg caaaatgcca ctctgaacct gagacattgt tcctcctgaa
    46981 aataataatg ccacgcaatc cgtggcattg ttgagcgcta ttttacgtgt tttttcgcgg
    47041 cggcggtttg ttcagacgca aattaacgcc gccagataat tttactgact ttccagtttt
    47101 tcagggtatc gtccgaaggc attaaccctt caggaccact ccattccccg ctgaactggt
    47161 agctaatatg ttgactgcct tcggctttac attcgacggc acgattatca tcaccatcag
    47221 ctttttggca attgccaaat gctttgctgt aaaggtcgct aaacggtgta ccgattttaa
    47281 caccggtgtc agcaggaata tcgctatcca gcacgtcaat gcgactgatc gtgccttgat
    47341 cgccgttaat caccatcgcc acgttgtcgc ccttcatcac ttcaaaaaag cgcaccacgt
    47401 tgccgttcgc ggttttcatt ccgctgcgca ggcgataatc gccatcaacc gcatcggcaa
    47461 tggcttgttc ttgcagtggt gtggacgccg ttaattcacc aacgccctgc tcgctcactt
    47521 tggtggacga cccaaaccag ttccacgggt tagcggcaga ccagttaact gacgacatcg
    47581 tggaacagcc ggtcagcatc aatggcatag cgcataacat taaacgcagc gatttcatgt
    47641 cacttccttt ggttattcaa taacgttgct tggagtgcaa attcaccaaa aagtgccgtt
    47701 attcttttac ttcattaaaa caagcgcgta aacgtcgatt ggtcagcagc cagattaacg
    47761 ccacaatatc cgccactacc agcgccagac cgataccgct aacggattca ccgttcagcc
    47821 acagccacgg ttgccagcaa agtaaaacca cctgcgccaa cagcaacaga aaatagagta
    47881 cacgccaggt gcgaggaaac gtagcccgcc gaccgctcaa caaaaacgcc agcaccgccg
    47941 gaatgccagg aatcagcccc agccagaaat tatcgtgatc gggataaaac agatttagca
    48001 gcgcagtacc ctgctcgcgc gacgcaccgg caatgacaaa cagcacccag gttcgcgcct
    48061 gaagcaatag cacaagccag aagagcaagg gtaaacgcag gcgaccgtgc gcatcataat
    48121 ggacaggatg aaactcagta ctcttcatct tcaatcaaac gcttacccag actcagcacg
    48181 tcggcgtgtt catatcccag gcgttcatac attccgagca ccatgtcgtt atcttccggc
    48241 acgttgatct gaattttcgg gcagccgcga gcaatcagct ttttctccag ccggttaagc
    48301 aacgcattgg caatcccacg cccacgaaac tctggatgca cgccaagata ataagcagac
    48361 ccgcgatgcc cgtcataacc gcccatcacc gttccgacca cttcaccgtt cacctcagcg
    48421 accagaaaca aactgacgtc atggttcatc ttacgctcga tgtccatttc cggatcgttc
    48481 cacggacgca gcaagtcgca acgctcccaa agggtgatga cctcttcgaa atcttcctgg
    48541 cgaaatacgc gtatctccat ggtattcgtt acctttttgc gggttaaaag gctgattatg
    48601 gcgtgaacgg tcgaattagc caatatctga cgaaaatcgg ttgaaaaagt ggcataatgg
    48661 ggagttgtca actattgaaa tgaaaagtaa aacaattctc aacagcaaac ggtcgtaacg
    48721 gattacgcga tacgatataa catctggaac tttattatta caactcaggc cgtatgagca
    48781 cttttaaacc actaaaaaca ctcacttcgc gccgccaggt gctgaaagcc ggtttggctg
    48841 ccctgacgtt gtcaggaatg tcgcaagcca tcgccaaaga cgaaccttta aaaaccagca
    48901 acggacacag caagccgaaa gctaaaaaat ctggcggcaa acgtgtcgtt gttctcgatc
    48961 caggccacgg cgggattgat accggagcga tcgggcgcaa cggttcgaaa gaaaaacatg
    49021 tggtgctggc gattgctaaa aacgtccgtt ccattttgcg taatcatggg attgatgcgc
    49081 gtttaacgcg ttctggcgat acgtttatcc cactttacga tcgcgttgaa atcgcccata
    49141 aacatggcgc agatctgttt atgtcaattc atgccgatgg ctttaccaac ccgaaagctg
    49201 ccggtgcttc ggtatttgcc ctctctaacc gtggggcaag tagcgcaatg gcgaaatacc
    49261 tgtctgaacg cgaaaaccgc gccgatgaag ttgccggtaa aaaggcgact gacaaggatc
    49321 atctattgca gcaagtgctg tttgatctgg tgcaaaccga tactattaaa aacagcctga
    49381 cgctcggctc gcatattctg aaaaagatta agccggtgca taaactgcac agccgcaaca
    49441 ccgaacaagc cgcgtttgtg gtgttgaaat cgccgtcggt tccttcggtg ctggtggaaa
    49501 cctcgtttat caccaacccg gaagaagagc gactgttagg cacggcggcg tttcgtcaga
    49561 aaatcgccac agcgattgct gaaggcgtga tcagttattt ccactggttc gacaaccaga
    49621 aagcacattc gaaaaagcga taagttatga aacccgacgc acaccaggtt aaacagtttc
    49681 tgctcaacct tcaggatacg atttgtcagc agctgaccgc cgtcgatggc gcagaatttg
    49741 tcgaagatag ctggcagcgc gaaggtggcg gcggcgggcg tagtcgggtg ttgcgtaatg
    49801 gtggtgtttt cgaacaggca ggcgtcaact tttctcacgt ccacggcgag gcgatgcctg
    49861 cttccgccac cgctcatcgc ccggaacttg ccgggcgcag tttcgaggcg atgggcgttt
    49921 ctctggtagt gcatccgcat aatccgtatg ttcccaccag ccacgcgaat gtgcgctttt
    49981 ttattgccga aaaaccgggt gcagagccag tctggtggtt tggcggcggc ttcgatttaa
    50041 cccctttcta tggttttgaa gaagacgcca ttcactggca ccgcaccgcc cgtgacctgt
    50101 gcctgccatt tggtaaagac gtttatcccc gttacaaaaa gtggtgcgac gattacttct
    50161 acctcaaaca tcgcaacgaa cagcgcggta ttggcgggct gttctttgat gatctaaaca
    50221 cgccagattt cgaccactgt tttgccttta tgcaggcggt aggcaaaggc tacaccgacg
    50281 cttatttacc aattgtagag cgacgtaaag cgatggccta cggcgagcgc gagcgcaatt
    50341 ttcagctcta ccgtcgcggt cgttatgtcg agttcaatct ggtctgggat cgcggcactc
    50401 tgtttggcct gcaaactggc gggcgcaccg agtcgatcct gatgtcaatg ccgccactgg
    50461 tacgctggga gtatgattat cagccaaaag atggcagccc agaagcggcg ttaagtgagt
    50521 ttattaaggt cagggattgg gtgtaactcc ctcaccccca ttcccgcatc cgctgatgca
    50581 acgtcaacga cggcttctcg gcaaacagct gctggtaatc cgtggcaaat tgccccagat
    50641 gccagaatcc ccactgcatg gcggcgtctt ttaccgttgt gctttgcgac cacgggctta
    50701 tcagttcgcg gcgtacggcg ttcaggcgaa tgcgtttcag ccacgcgttc gggccaattc
    50761 ctaaaatagc gtgaaacgcg ttttgtagcg tgcggcggct gacatgcagt tgattacaca
    50821 agtccagcac cgtcaccggt tcggacatgt tttccagcac atattcacgg gcgcgggaaa
    50881 gcaaccgacg gtaactctga tgactgatgc tttccgccgt caccattggc tgcgcttctt
    50941 ccagcatcgc ccccatcgcc attagcaaat tatcccccag cacttttcgc actgcaggct
    51001 gatggagatt ttccgggttc tcgcaaaacg tcgccagcgc ctgttggaca aagccccaca
    51061 gcgcggcttt atgctgctct ttcacttcca gcgccgactg gctacgcaac atatgtaata
    51121 cccgatccgg attatgcaaa aagttagcct gacgggtgat gacatcttca gaaagcacta
    51181 cgcccaggat cgtgtaatcg tccggcgtac ttaattcaaa ctcggttcca ccagggcgcg
    51241 tcgctatttc tgcgcttccc agacattgcg aaccgataaa tccctgctca ccgcgcgtcg
    51301 ccggaatgcc aaaccagaac gagttcggcc agaccagaca cgactgacgc agcgccagac
    51361 cggtgtattc acgaaaaacc tgaatatcat cgagtagaat ttccgtaaat tcaccatgaa
    51421 acttgcccgg atgcagctga tcgtaaatct gctgccaggc ggtaatcgtt aaagcatgtt
    51481 catagacatc cgttgtctgt cgttgatgaa cattatccac ttcgaccttc ggcgtgagct
    51541 tcaggttttc gggtaagggt tcatgataaa gatggtgcaa attggctgta cgggtctttt
    51601 tcatgatgtt aatgccgggt gttgtaggac acccggcacc tccgacaggt taatggggct
    51661 tgagacgata acgactactg cgtttacgta acgtcccagc agaaaagagc tgttcgagcg
    51721 catttctggc tttttccagc ggccagccaa agtgggcagc cacttctcct gccgtcactc
    51781 cctgacgtac tgatgttaac agcgccaaca actcgtcagc agattccgcg acgataactg
    51841 gcgcgtcggg ttcctttacg ggttgcttcg gctggcggtt aaagccagta accagccact
    51901 gggtgtcatc gtctggccga ccgatctcct tacggctgat gacacgcccg gtacgcatcg
    51961 cggcagcaca acctgcgtcc agcgcagcac gacacgccgc cagatcgcct tccaccacca
    52021 gcgttaagcg acctgggtca agcacttcgt gactgagcag acgaacgtta gctgctttga
    52081 gcatggcatc cgctgcatct atcgcggcga ccatgccgtc cacttccagc aatcccagtg
    52141 cattgatcat tggcaacctc cgttacgcac gctggattgg attacgcgcg atttccagca
    52201 ctgcatcggt aaaggcgtta caggccgctt tacacgctgc ctggctaccg gttaaaaatg
    52261 ctgccgagta gttagtttca gacggtggcg ggacataggt caccagctgt acgtcggcag
    52321 atttcaacgc tgcatcaatg ccgtaggtcg cttccagcgg cggtgccacc agatacgcca
    52381 tcggatcgcc aagcgtgatc ccggcggttg atgagagata agaaccggta cgcgaaacta
    52441 catgtgccag gaatgccgta tcttgcgcat cattagccca ctggaaagca gcgccatttt
    52501 caatatgcgc aaccatcgca tccagaccgg cacgcacttc cgccgggttt ggcccaccaa
    52561 gcataatcag cacctcaccg gcagtcggtg acgggccgtg tgccgcgccc gcatacagcg
    52621 agcgaccata taccacttct accatcgctt gtttggtcgc ttcgtcagcc gcaatgtagg
    52681 ttacgtcgtc agaatcggca gaaatcagac cgaggctacg aatatgtggc ggcaatttca
    52741 gttcacgcgc aaaatcggcg ttaacggagg caatcacccg catggcggtt accgacggtc
    52801 gaatcaaatc taaagctggc atgatgcctc cttatcgggt catgttgatg ccggatgctt
    52861 tctgctccag catacgtttc gccaaatcca caatgacggc tgcggcttca accggcggcg
    52921 tgccgccctg gtgaatgtta gagatacagg tgcgatcggc ctcaacggtg gtcgccatac
    52981 gcggcgagta aacggcgtag caggagaggc tttctgactg acccaacccc ggacgttcgc
    53041 ccaccagcag gatcactact ttcgcgccga ggatctcacc gatctgatct tcaatcttca
    53101 cgcgaccata acgaacaaag aatggcgtgc cgactttcag cccggcctgt ttcaggcccg
    53161 ccatcagcgg cggcaggatc tcttcgtagt tcacagtgat ggcatcagtt gacaggccat
    53221 cagaaataac gacctgtacg tccggattag caacacactg tgctttcagc gcttcaacag
    53281 cttctgcaca caggcggcgg cccatatccg ggcgagtcag gtagagattt ttgtcgctaa
    53341 tttcggagcg tacttccagc aagccctgcg ctttcaccca ctcttccggc acttctttca
    53401 gtacggtatc tttcgaacga gagtgatcgg ccaggaaacg cagcagcgcc agcgtacgcg
    53461 gacgcggacc ggcacgaccg gtacaaacgc gggccacggt gctgcgacgc agttctgtta
    53521 acacatccgc acgatgtggg ttttcaacac caatccacac ttttgcttcc gcagaaccta
    53581 aatccagcgc gcagctttcc gaggtcaccg gtgccgcaca ggtggtagtg gcgcactttg
    53641 cttctgacgg cgcgggggcc gtttgtccca ttgacgccat cacgctgcgt acaatttctt
    53701 caatctgttt ttgatccatg atgtgttatc tccgcgtcat cagaagaaca gtgacggatc
    53761 gcccgcccgt ttggtcaggc gaccgtttgc cataatgccc atgctttcca gccagcgttc
    53821 aaactccggt gacgggcgca ggttgagtaa ctgacgcaca gtggcggtat cgtggaatgc
    53881 ggtggtctgg tagttgagca tgatgtcatc acccagcggc atccccatga tgtagttgca
    53941 gcctgcggtg gcgagcagga tcatcaggtt ttcgttgagg ttctggtcag cgtcagcgtg
    54001 gttggtgtaa cagcagtcac agcccataga gatgccgctc agcttgccca taaagtgatc
    54061 ttctaagccc gcacggataa tctggcggtc gttgtagaga tactccggcc caataaagcc
    54121 gaccacggtg ttgacgataa acggatcgta atgacgcgcc agcccatagt tacgtgcttc
    54181 catcgtcacc tggtctgcgc cgaagttagc gccagcggat agcgctgaac cttgtccggt
    54241 ttcgaagtag aggcagtttt ccccggcgat acagttgaac tctgcaccca ctgcgcgcgc
    54301 ttcgtcgagc atcgccagtt ccacgccaaa ctctttcagc cctttttcgc tgccacagat
    54361 actctggaaa atcagtccgc caggcgcgcc gcgacgaatc gcttcgatct gggtggtgac
    54421 gtgcgccagt acgcagccct gagttgggat gttgaatttg tcgatcacgc catagatggt
    54481 gtccagcacg cggcttaagt tttccacgtc gtcagtcacc gggttaacgc cgatcaccgc
    54541 atcgcccacc ccgaaggaaa gcccttcgta gatttgcgcg gcgatacttt gcacgtcgtc
    54601 acgggtgtca tttggctgca aacgggcgct gaaggtgccc ggaataccga tggtggtatt
    54661 ggcctttttg attaccggca ttttcttcgc gccgtagatc aggtccgcgt tggagcaaat
    54721 cttcgctacc gccgcgacca cttccgaggt cagccctttg cgggtaaagg caatgtcgtc
    54781 cacgctggtt tcatcgctca gcacatactc acgcagttcg ctgatgctcc agtttttaat
    54841 ctggttgtag gccgtttcgt taacatcgtc ctgaatcagc cgcgtcacgc agtcatcttc
    54901 ataggcaatc accggattat tgcggatgtc cgctacggtc atttccgaca acacctgctt
    54961 tgccgccacg cgctcctgtg agcttgccgc tgcgacgccc gccagcacat cccccgaaca
    55021 cagttcgttg gctttagcca gcacctcttt tacatcctta aactgatata cattgccgaa
    55081 caatgtggtc tttagtttca taagtcgttc cctcaggaag gaaatgcgag tgatttcacc
    55141 gtcaccggca caaccgatcc gccaaaaaga ggcgtaccaa tgtcgatata gtcccccgcg
    55201 cggacaatca cttcgtcaat gactgccaac gggagttgtt gtagctgtgg gcgcaacagc
    55261 atgcccagag ctttaccaaa gtcctgcccg gccaccacca gcaggggatg cggattcgga
    55321 aaacgcgcga cgaaatcgac caacgcgttg atgaccgtca gtaccgcggc gtaacgcaca
    55381 ggcagcgagg cgggaagcgc cagcacgtac gcgtcagttt tgggatcaag atccagctga
    55441 atcagcgcct gttgccaggc actcaccaga tccgtttcat caatcgggat cgccaccggc
    55501 aaattgcgca gcggcagttg tacgccctcc agccagattg tgctgccaga gagcgaaagg
    55561 gtatgtgcgc ccgcgccaat caccgtggcg cgcacggttt gcgccggaaa ctgcacgttc
    55621 atctcacgca ggcgcggatg gtcatgtagc gccgtcgcca gtagcgggcc aatatcggca
    55681 aaacagaacg ggtcagcggg ctggtggcga taacattcgc ccacgccgcc agaaagcgta
    55741 atgatttcgg gcgtaacacc tgcgggcagc aaaccggttt gcattaatgc ctgcgcgagc
    55801 ggcgaaagcg ttccgtcaat cacttcgaca atcagctctg ccatccgtcg ggtaacctgc
    55861 accagctgcg cgccggtcag cgaacggacg tcagtgcctg caccgaaaca ctcatccaca
    55921 atcatctgcc ccggtttatg agcgtaaacc acgcgcccct ggctgtcggt ttccagcagg
    55981 cgaccaccga cgttgaggca ggcagtgccg ctgatttttc cggcatcgaa cagggcgtag
    56041 ttcgcggtgc caccgccgat gtcgatattc agtacccgac acagccgttg ttcagaaagg
    56101 gtttgcgccc cggcaccgtg accggcgatc acggattcga ggtgcggccc ggcgctggcg
    56161 acgacaaaat cgcccagcga ctgagagagc gccatcaccg ccgggcgagc attgcgggtt
    56221 ttcgcgcttt caccggtgat gatgatggca ccagaatcaa cgctttccgg cgcaatgccc
    56281 gcagcctgat attgctctaa aatcagcgat ttcagttccg cttcttttaa accaccctgt
    56341 ttatcgacag gggtaaagaa caccgggctt tgccagctaa tttcgcgttt gatgaattcg
    56401 tagcgcggca cctgcgacac cgccgcacgg ttaaccagtt ccaggtggga gaaaatcacc
    56461 tgggtggtgg tggtgccgat atcgataccg acgctcagta gctggcgagt gttcacgatt
    56521 gtgcctccgc ttcggtttta gccgcggtcg cgtcgtcttt tggcaccagc atcatcgcca
    56581 cgccaatcgc cgttacgccg ccgatcaact tgccgacaat catcgggaag atcatggcat
    56641 tcatgttggc agcggcgaag cctaagtggt cgcccagggc gaaagcagcg gaaacggcga
    56701 aggcgcagtt gatgactttg ccgcgggtat ccatctgctt catcatgccg aacatcggga
    56761 tgttgttggc aagcgttgcc accatgccgg ctgccgcgat gttgttcata ttcagcactt
    56821 taccgacgct catcagcggt ttttcaaacc agcgggtcag cagcagcacc atcggatacg
    56881 cccctaacag aacgcaggag atagaaccga taacttcaat ggcgcgcatc acctcaccgg
    56941 gtttatcgcc aggggccata aagataggat ccagaccggg gatcagttcc cagccaagca
    57001 ggaatttcac taccgcagcg gcaagaccga gggtgatcaa tgcaacgagg aatttggcga
    57061 agatctggaa gccgttgatc attttttctg ggatgaattt cagccccagc gccaccagaa
    57121 tcgcaacaat gatcaccggg atcatgttca tcaggatcag ggcgaaagtg aattccaccg
    57181 gctggccgtt gatctgaaca ccggagtaca tagcaaccag accaccagcg atacaaccaa
    57241 tcggaatggt cacaatgccc gccagcacgc cgagcgccag ataacgacgg tcagaaggtt
    57301 cgataatgcc gagcgccacc ggaatggaaa acacaatcgt tggccccatc atcgacccga
    57361 gaattaaccc agagtatagc cacgcggcta cgtcgccgcc cgccagctct ttggcgagga
    57421 agaagccgcc catatcgcac gccagcagtg ttccggcgaa catcgatggg ttagcgccga
    57481 gcatttcgta aaccggaata attaccggcc cgagtacgtg agccagtacc ggtgccagcg
    57541 cggtcatacc gaccatcgcc aggcccagtg cgcccattgc cataaagcct tcttcgaact
    57601 gaccgccgga tccttcgata cttttaccga acttaccgag gaaacgagca gaaccgccga
    57661 actgtgacag gatcctgtca acggcagcta tcagcataaa gaacatcatg atgtacatga
    57721 tgatttcgtt aattcccatc gcctttactc cctgttagtt gttatttatt agcggatgct
    57781 gcgtgaagcc ttatccgcac tacatgtgca atcccacaga ccggataaga cgcggcattt
    57841 attgcgccgc tgcgtacagg ccgacaagct gttccaggct ggcggtacgc gggttgctgc
    57901 gcagacaaat atcttccagc gcggcctgcg cccatgcacc gtaatgcgca gatgtcgcgc
    57961 caacatcgcc cagtcgttta ccaatcccaa cttccgcaat cagctcactt accgcgttaa
    58021 tagcgtcacg atcgtcggat tttttagttc gcagtgcccg accaatctga ctaaagcgtt
    58081 cgcgacaaac catccggtta aactccatca ccgttggcag caacatagcg ttcgcgagac
    58141 cgtgcggaat atgcagcgcc gcgcccggct gatgcgccat cgcgtggcac aacccaagac
    58201 ccgcactgga aaacgccatt cccgccatac atgaagccag caacatgctc tcgcgcgcgg
    58261 caaggtcgtg accgtagccc accgctttcg gcagcgattt gccaatcatc gcaatggcac
    58321 caatcgccag actgtcggta aacggtgtag cgttcagggc gctgtatgct tcaatggcat
    58381 gggttaacgc atcaatgccg gtcatcgccg tgacatgcga cggcacacct tcggtcaatg
    58441 cggcgtcgag gatcgccaca tccggcatca gcgaggcatg ggctaacacc tgcttgcgcc
    58501 cgctcaccgc gtcgataatc accgttacat tggtggtttc agagccggtt ccggcggtag
    58561 ttggaatggc aatcagcggc aagcgcggtt gcagaacgct ggtttctgac atctctgcca
    58621 gcgtgctatc cgggttcgtc accagcaacg ccacggcttt cgccgcatcc agcaccgagc
    58681 cgccgccaaa tgcgatcacc ccgtcacaac ctgactcacg caactgcgcc acggctgcac
    58741 acacgtcggt aatgcacggt tcgcccaccg gacatggcca gagcgtcatg gcgatacctt
    58801 taacggccag gctacgcgtc agcccggcgg tcatccctgc ctgatgcaaa aagctgtctg
    58861 ccatcacgaa cagatgtttc agcccacgcg tttgcgcttg ctgtccgcaa ctgctcaccg
    58921 agcccggacc gcaaagcgtc accggtggaa cgctaaatgt ttttacccgt tgcagattca
    58981 gggtatcgaa cgcctgaaag agcgcggtct gcaattcatt ttgcatatag cccctccgct
    59041 ttctctcgcc cactactggc gatcgccagc ggggtcataa acaaactgtg ctgcggtaaa
    59101 tgcacctgta acgccgggaa ttgtttgcga aacagttctg ccacgcccgg ttgcatacag
    59161 gagccgcccg ccagccataa atcagtaatg ccctgccctt caatatgccg ggcgacaatg
    59221 tccgccattt tttcgtacac cggtttcacc gtaggccaaa tctcttcacc gtgaccgcgc
    59281 ttgtactgct ccgcctcttc cagcgaaata cggcgatttc cggcgaaagt cagagagatg
    59341 tgatgtccgc cggtggcttc atccgccgag tacgtcactt tgcccttttt cacgatggca
    59401 atgccggtag tgccaccgcc gatatccact acaccggcgt tgtccagttg cagcagatcc
    59461 gcgactgcgg ttggctcatc cagcacatgg ctcacttcca gcccggcaga ctccagcacg
    59521 ttgatggaaa tacgcgggtc cgtacctgga ggaaatgaag tcgccgcatg gctaaaacga
    59581 cgaccgaatt gctgctcgag cgtgtcgaga tggcgacgaa caatggtgac cgcgccgaag
    59641 aaatcccaga cgatgccgtc gcgaaccaca tcggcccagt cgaggcatac cgccactggc
    59701 tgaccgtcgc ggtcgacaac catcgacacc acatcgcagg tgcccagatc cactcccagc
    59761 cacagcggag attccgtcgc ggcgggcgtc tggttacaca gcgttgccgc cgtttgcaga
    59821 cgtggggtga gccattgttc gtcgtgcgcc atctgttact ccttaaacaa tgcgaaacgc
    59881 atcgactaat acacagcgac gcagacggac aaacgtacgc gcgctggtca ccccttcacc
    59941 ggttggcgtg gtgatggtca tggtggtcca gccttccccg cccagcccca gcccggcaat
    60001 gcacggtccg ttcttaacga aaatgctggt atcaacagca ttcgccatct ggttcatgtt
    60061 ttcgatgttg cgcgagtgca ttgccgccgt gtggtggcaa ccgccttcca gtttcaccgc
    60121 tagcgcaatg gcatccgcca cgttggcgac gcgcacgacg ggcaacaccg gcatcatcag
    60181 ttcagtcacg gcaaacggat gttctgcggt ggtttccaca aacagcaggc gcgtttcttg
    60241 cggaacttta aggccgattg ccgccgcgat tttgcctgcg tcgcgaccaa cccagtcacg
    60301 gctgacggtg ccttttccgc gctcgtcgat atttttcagc aacaccggtt gcagctgctg
    60361 cgcctgttct gcggtcagtt tcaccgcgtg ctggccttcc atcagacgca tcagttcatc
    60421 ggctacgcta tcaacaacaa tcagtacctt ttcgtcggca caaatgatgt tgttatcgaa
    60481 agacgcgcct ttgacgatgg actgagcagc acgggcgagg tcagcggttt catccaccac
    60541 taccggcggg ttgccagcgc ctgcggcaat cagacgttta ttggtgtgtt tacgcgccgc
    60601 ttctactacc gcttcgccgc cggtcaccac cagcaggccg atacccggaa acttgaacaa
    60661 gcgttgcgcg gtttcgatat ccggatttgc cacagtaacc agtaagtttt ccggcccgcc
    60721 tgcggcaaca atcgcctggt tgagcagcgt gatcgcccgc tgggagactt ttttcgccgc
    60781 cggatgcggg gcaaaaatga cgctgttgcc cgcggcaatc aggctgatgg cgttattaat
    60841 tacggttgcc gccgggttag tggacggcgt caccgaagcc accacgcccc agggcgcgtt
    60901 ttcaattaaa gtcaggccgt tgtcgcccgt cagcacttgc ggagagagac actcaacgcc
    60961 tggcgtgccg cgtgcctgag cgacgttttt ggcaaattta tcttcaacgc gacccatgcc
    61021 ggtttcactg acggcaagtt ccgctaaatc tctggcgtgt ttttcgcctg cttcacgaat
    61081 ggcagcaatg gctaactggc gcattgccac gctctttaac ccttgctggg caattttggc
    61141 tgccgcaaca gcgtcatcca gggacgcgaa aacgcccatc tcatgaacgg cggcgggcgg
    61201 cgtgtcactg ctttgcattt tcagcagtac cgctttcacc acctgttcaa tatcctgttg
    61261 attcatgatg ttctgcctta tttgtggaaa attacctgac cgccagacac cacctcatcg
    61321 acgatgccaa tcacgcacag atcgaccggt gacgtttcgc ttttatgcgc ctggcgggcg
    61381 gaactgccac tcaccagcaa cacccactcc ccggttcccg cgccaatatt gtcgatggcg
    61441 acggcacatt gcccgtcagg attaccatgt ggatcaatca tttccaccat cagcaatttg
    61501 tcatgcgcca gtccgtgatg gcgtacggta caaacaattt gtccagtgac gactgccagt
    61561 ttcatacccg cctccgtggc gtatttcagg taaaagctcc ccctaccctc cgcggaaggt
    61621 aaaatgaaaa atgagagagc gtgacgcccg aatcgacgtc acacagggtg attacaggtt
    61681 gctgctatcg cctttcaggc cgatcgggaa cacttcttcc aggtcgccgt gcggacgcgg
    61741 gataacgtgt acagatacca gctcgccgat acgctgtgcc gctgcggcac cggcatcggt
    61801 cgccgcttta caggctgcaa catcaccacg aaccatggca gtacacaggc cgccgccaat
    61861 ctgcttaaca ccaaccagct tgacgcgcgc agctttcacc atcgcgtcag aagcctcaat
    61921 cagtgcaacc aggccccggg tttcgatcat tcctaatgct tccattgtgc tttcctcttt
    61981 atcagggtcc agaacgggac cgttcattca accagtgttt gtaaactgct ttcgcggttc
    62041 acttctgtct gacgcggcac agctgccacc agcgccagct cgataatttc ctgcacacta
    62101 caaccacgag agagatcgtg catcggcgcg gcaagtcctt gtatcaatgg cccgacggca
    62161 cgatatccgc cgagtcgttg tgcgattttg tagccaatat ttccggcttc cagcgacgga
    62221 aaaaccatca cattggcctt gccctgtagc gggctggcag gcgctttttg cgccgccact
    62281 tccggcacga aggcggcgtc aaactgcaac tcgccatcga ctaccagctt tggtgcgcgc
    62341 tcacggacga tttctgtcgc ctgctggaca ttggcaacgc aggggtgacg ggcgctaccg
    62401 ttactggaaa acgacagcat cgccacgcgc ggctcttctc cggtgatggc gcgccaggtt
    62461 tcggcgctgg caagcgcgat atccgccagc tgcgccgccg tcggctgtgg taccacgctg
    62521 caatcggcaa agcccaacgc cggaccgctg tactgtggca gcatcaggaa aatggatgag
    62581 agcgttttac agcctggctg caagccgata atgcgtaatc cggcacgcag cacattcgcc
    62641 gtggaagaga ggttgcccgc gatacagaca tccgctttac cggcgctgac cattgcggcg
    62701 gcgaacatca gcggatcggt aagtttttcc agcgcatccg gcggcgtttt ttcgcccgcg
    62761 cgggccagcc agcgatgagc aaattcttcc cgcattgcga ggttgccatg cggatctatc
    62821 acctgtagcc cgtccatcgc cacgccgtga ctgagcgcaa actgacgaag ttcaaacgga
    62881 ttggcgacca gaatgggcgt tgccagacct tgttgatgta aatattgcgc agctttcagc
    62941 acacgttgat ctaacgcatc cggaaaaacc actctggcgg gcgctcgcaa cgccagttca
    63001 caacaacgtt caataatcat tgcgtttccc ccagtcgttg ttggatctgc ttcaccgtta
    63061 gcggctgctt cgttacgctc aggatcatca tcacgtagac cgtgctggaa aggcggttca
    63121 gcgcctgcaa gatgtccggg cgtaatactt caaaactgcg ggtgataaac acctgcgccg
    63181 ctacggtttc ggcttcccgc actttggtgc gtagcagatt cagcaacgcc gcatcgcgcc
    63241 cgtggcttgc ttccggcacc agatgatcgt gaccaagata acgcaacggc tgatgagaaa
    63301 gccggtgcag atcttcgtca gaaagcccga caatcgcctg gcagcccagc ggttccgcca
    63361 gttcaatttg cagccacact gccagcgcaa tggtgctgtc gagaactgcg cgaaaaccca
    63421 gacgcggatc gcttttggcg accatttttt ccgccgacag gtgagtcagc gtatccggct
    63481 tttttgccac cggctggcgg cacagttcgc agcacgcctg cggatgttca tcgctactgg
    63541 ttagcccatg aacgggctgc ggctgctgct gttcatcgtc aacaaacagg cggccctgct
    63601 cgtcaataaa cttgatgcgc agatggcggc tttccagcaa ctcccgggca gagggcgtca
    63661 ggcgactgtc cgcagggaga tggatctctg cgccttcgct gagcgtatgg ttcgctctta
    63721 gccatgcttc ggtgatgaaa tctttcatag ggattgccag ttagccggcc aggcgacata
    63781 caggaatttc acgctggatg tcgtaccaaa ttcgatgctg gagcctttcg ggataaacat
    63841 cacgtcgcct gctttagcga tcatggtttc gccttcgtgg cgaacatgga gctccccttc
    63901 cagcaccatg tcgatttcgt cgtagttcag ggtccacggg aagaatgcgt tctcccactg
    63961 cataaaaccg gcggccatgc tgctgccgtc gtctccggtc acaagatcag tcaagcccac
    64021 gcaatgcggt tgtgcgccat caaagcgacc aaacttgacg ctgctaccat caatcacttt
    64081 gatgccgcct ttgccggtca ccgatttgaa gctcggctgc attgcaccct gctccagcga
    64141 ctgtttttcc ttcatcactt tttccatcag ctgcgcgacc aggctttcgg taaactggcc
    64201 ttccggtagc tgggcgataa tggtttcgcg gatgcgctgg ctttcggttt tatcggctgg
    64261 cacgctggcg ggcacggacg ccgttaccgg gatcaattca tcacattcgg tgatggtaaa
    64321 acccagtaga tccgccactt cacgggcttc cggggtaata atgctggcgc gcagaaccac
    64381 ggacattgcc tgttcgccgc gcgcgtgtgc ttcacgaata tcattcgctg tgatgagttt
    64441 tttcacctgc ttcctcctgt ttggtgagag atgccagata atccaccagt tgctgcacgc
    64501 tttgcgggtc gtggctgttt agctcgaaaa tcggctcctc gaagcccgtc tcaagcaata
    64561 actttcgcgt tgcggcgacg tcagcatccg gcatgtccgt tttactgatg acggcgattt
    64621 gccgtttgct gacgccaata tccagcaacc cggcaggcaa ccgactttcc gggtcattcg
    64681 cgccgtggac atagatcagc atgtccacat cctgtagcgt ggtaattaag gcgtgatacc
    64741 agcggggatg gctaaaatat tcacccggcg tatcaatatc gcccttatca ttaaattcca
    64801 cggcctgtgt ttttctggcg agggtataat tcccctgcaa cgcattaaat aacgttgttt
    64861 tccctgcccc gacggtaccc acaaacgcaa ttcgtttcat gaatgcctcg tattaacttt
    64921 tggtcatttc acaaagcgta taatttaata agcgacctaa accactgacg gtttgtgata
    64981 acgcttcttc tactgcgccc accgagccat aaatcaccaa tgcaccactg aagcggtcga
    65041 gaaatccgat atgcacatcg gcggctttca gcgctaaatc accggcgatc atcgccgttt
    65101 cgccgggagt tagcgtcata atgccaattg cgcccgcatc cggaacgccg attttcttcg
    65161 ccagttcctc gccgggatgc gcgatgagat gcgccagtgt gacctgtttg ccgggcacaa
    65221 attcctgaat gatgcgttct ttatccatga gtcgccgcct gtgaaaaact ttcgtgtatc
    65281 gtgacaattt ttcgtcagag taaataacaa aat
//
LOCUS       DBFIFI010000008        61822 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.008, whole genome shotgun sequence.
ACCESSION   DBFIFI010000008 DBFIFI010000000
VERSION     DBFIFI010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 61822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61822
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.008"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            67..633
                     /locus_tag="N7B39_000495"
     CDS             67..633
                     /locus_tag="N7B39_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGU2420539.1"
                     /translation="MTSKLEIRHKQRQDEIINAARRCFRRCGFHAASMSQIASEAQLS
                     VGQIYRYFANKDAIIEEMVRRIIDFRIAQMDIDARTDHFPEVLALRKSLNEDDDALML
                     EVAAEATRNPRVMAMLEEADARMFANGCAHMKRMHPHLSEEHIRCCVEVFATMMEGTV
                     YRRLTPQKSDPQHLQEIYQDIVSMLINK"
     gene            782..1903
                     /locus_tag="N7B39_000496"
     CDS             782..1903
                     /locus_tag="N7B39_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HGU2420540.1"
                     /translation="MKYIATSVVAMLLLSGCDNTQSNNSSPSETEVGVVTVKSQPVSV
                     VSELTGRTSAALSAEVRPQVGGIIQKRLFKEGDLVKAGQPLYQIDAASYQAAWNEARA
                     ALQQAQALVKADCQKAQRYARLVKENGVSQQDADDAQSTCAQDKASVAAKKAALETAR
                     INLDWTTVTAPISGRIGISSVTPGALVTASQDTALTTIRGLDTMYVDLTRSSVDLLRL
                     RKQSLATNSDTMSVSLILEDGTTYSEKGRLELTEVAVDESTGSVTLRAIFPNPQQQLL
                     PGMFVRARVDEGVMEDAILAPQQGVTRDAKGNATALVVNKDNKVEQRTLETGETYGDK
                     WLVLNGLHSGDRLIVEGSAKVTSGQTVKAVEVQANGGNA"
     gene            1903..5011
                     /locus_tag="N7B39_000497"
                     /pseudo
     CDS             1903..5011
                     /locus_tag="N7B39_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133655.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
     gene            5015..6388
                     /locus_tag="N7B39_000498"
     CDS             5015..6388
                     /locus_tag="N7B39_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HGU2420541.1"
                     /translation="MLRRSLIFLVLLSAGCVSLDPHYSTPESPIPATLPGAQGQGKAI
                     SHDWQQVIHDPRLQQVVTIALNSNRDVQKAIADIDSARALYGQTNASLFPTVNAALSS
                     TRSRSLANGTETTAEADGTVSSFTLDLFGRNQSLSRAARETWLASEFTAQNTRLTLIA
                     EISTAWLTLAADNSNLALAKETMTSAENSLKIIQRQQQVGTAAATDVSEAMSVYQQAR
                     ASVASYQTQVMQDKNALNLLAGTTLEENLLPGTLESLPEQMISLVPAGVSSDVLLRRP
                     DIQEAEHNLKSANADIGAARANFFPTISLTASAGVGSDALSSLFSHGMQIWSFTPSVT
                     LPLFTGGSNLAQLRYAEAQKRGLIATYEKTVQRAFKDVANALARRTTLEEQLDAQRQY
                     VKAEQQTVDVGLRRYQTGVGDYLTVLTAQRSLWSAQQELLALQLTDFTNRITLWQSLG
                     GGMSSLK"
     gene            6397..7560
                     /locus_tag="N7B39_000499"
     CDS             6397..7560
                     /locus_tag="N7B39_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HGU2420542.1"
                     /translation="MARVSLSWALILGLLAGIGPMCTDLYLPALPEMSEQLAATTTIT
                     QLTLTASLIGLGVGQLLFGPLSDKIGRKRPLILSLLLFIVSSILCATTNNIYWLVVWR
                     FIQGIAGAGGSVLSRSIARDKYQGVTLTQFFALLMTVNGLAPVLSPVLGGYIVSTFDW
                     RTLFWVMAEISTVLLLGCVLFINETLPENKRGSSLLLTGRSVVQNRRFMRFCLIQSFM
                     LAGLFAYIGSSSFVLQKEFGFSPMQFSLVFGLNGIGLIIASWIFSRLARRINAMTLLR
                     GGLIAAILCALLTVLCAWTQLPIPALVALFFTIAFCSGIGTVGGAEAMSAVGTQESGT
                     ASALMGMSMFVFGGIAAPLSGFGGETLLKMSLAITVCYTLAFLVALTRIDNQK"
     gene            complement(7612..8418)
                     /gene="sapF"
                     /locus_tag="N7B39_000500"
     CDS             complement(7612..8418)
                     /gene="sapF"
                     /locus_tag="N7B39_000500"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HGU2420543.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTNLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            complement(8420..9412)
                     /gene="sapD"
                     /locus_tag="N7B39_000501"
     CDS             complement(8420..9412)
                     /gene="sapD"
                     /locus_tag="N7B39_000501"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HGU2420544.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            complement(9412..10302)
                     /gene="sapC"
                     /locus_tag="N7B39_000502"
     CDS             complement(9412..10302)
                     /gene="sapC"
                     /locus_tag="N7B39_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HGU2420545.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCM
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            complement(10289..11254)
                     /gene="sapB"
                     /locus_tag="N7B39_000503"
     CDS             complement(10289..11254)
                     /gene="sapB"
                     /locus_tag="N7B39_000503"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HGU2420546.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            complement(11251..12894)
                     /gene="sapA"
                     /locus_tag="N7B39_000504"
     CDS             complement(11251..12894)
                     /gene="sapA"
                     /locus_tag="N7B39_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HGU2420547.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTAWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKLLGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKTLSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            complement(13207..13452)
                     /gene="ymjA"
                     /locus_tag="N7B39_000505"
     CDS             complement(13207..13452)
                     /gene="ymjA"
                     /locus_tag="N7B39_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2543 family protein"
                     /protein_id="HGU2420548.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVADAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            complement(13586..14971)
                     /gene="puuP"
                     /locus_tag="N7B39_000506"
     CDS             complement(13586..14971)
                     /gene="puuP"
                     /locus_tag="N7B39_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HGU2420549.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVAMSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            complement(14961..15125)
                     /locus_tag="N7B39_000507"
     CDS             complement(14961..15125)
                     /locus_tag="N7B39_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2420550.1"
                     /translation="MPMIKSPHGAGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            complement(15274..16692)
                     /gene="puuA"
                     /locus_tag="N7B39_000508"
     CDS             complement(15274..16692)
                     /gene="puuA"
                     /locus_tag="N7B39_000508"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HGU2420551.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            16904..17668
                     /gene="puuD"
                     /locus_tag="N7B39_000509"
     CDS             16904..17668
                     /gene="puuD"
                     /locus_tag="N7B39_000509"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HGU2420552.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPERDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            17695..18252
                     /gene="puuR"
                     /locus_tag="N7B39_000510"
     CDS             17695..18252
                     /gene="puuR"
                     /locus_tag="N7B39_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HGU2420553.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIILTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            18402..19889
                     /gene="puuC"
                     /locus_tag="N7B39_000511"
     CDS             18402..19889
                     /gene="puuC"
                     /locus_tag="N7B39_000511"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HGU2420554.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLANIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLIEANAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVVLKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQQAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIQEGESKGQLLLDGRNAGLAVAIGPTIIVDVDPNAS
                     LSREEIFGPVLVVTRFTSEEQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            19891..21171
                     /gene="puuB"
                     /locus_tag="N7B39_000512"
     CDS             19891..21171
                     /gene="puuB"
                     /locus_tag="N7B39_000512"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HGU2420555.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLEMLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTATGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSDDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            21209..22474
                     /gene="puuE"
                     /locus_tag="N7B39_000513"
     CDS             21209..22474
                     /gene="puuE"
                     /locus_tag="N7B39_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HGU2420556.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERAMQLGQRLTKTL
                     IDAKENVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALRD"
     gene            complement(22605..23582)
                     /gene="pspF"
                     /locus_tag="N7B39_000514"
     CDS             complement(22605..23582)
                     /gene="pspF"
                     /locus_tag="N7B39_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HGU2420557.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            23749..24417
                     /gene="pspA"
                     /locus_tag="N7B39_000515"
     CDS             23749..24417
                     /gene="pspA"
                     /locus_tag="N7B39_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HGU2420558.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            24471..24695
                     /gene="pspB"
                     /locus_tag="N7B39_000516"
     CDS             24471..24695
                     /gene="pspB"
                     /locus_tag="N7B39_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HGU2420559.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            24695..25054
                     /gene="pspC"
                     /locus_tag="N7B39_000517"
     CDS             24695..25054
                     /gene="pspC"
                     /locus_tag="N7B39_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HGU2420560.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            25063..25284
                     /gene="pspD"
                     /locus_tag="N7B39_000518"
     CDS             25063..25284
                     /gene="pspD"
                     /locus_tag="N7B39_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HGU2420561.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            25359..25673
                     /gene="pspE"
                     /locus_tag="N7B39_000519"
     CDS             25359..25673
                     /gene="pspE"
                     /locus_tag="N7B39_000519"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HGU2420562.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATVVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            25883..27561
                     /gene="ycjM"
                     /locus_tag="N7B39_000520"
                     /pseudo
     CDS             25883..27561
                     /gene="ycjM"
                     /locus_tag="N7B39_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
     gene            27575..28867
                     /gene="ycjN"
                     /locus_tag="N7B39_000521"
                     /pseudo
     CDS             27575..28867
                     /gene="ycjN"
                     /locus_tag="N7B39_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            28888..29769
                     /gene="ycjO"
                     /locus_tag="N7B39_000522"
     CDS             28888..29769
                     /gene="ycjO"
                     /locus_tag="N7B39_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGU2420563.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVIFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            29756..30598
                     /gene="ycjP"
                     /locus_tag="N7B39_000523"
     CDS             29756..30598
                     /gene="ycjP"
                     /locus_tag="N7B39_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGU2420564.1"
                     /translation="MATNKRTLSRIGFYRGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPVIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            30629..31681
                     /gene="ycjQ"
                     /locus_tag="N7B39_000524"
     CDS             30629..31681
                     /gene="ycjQ"
                     /locus_tag="N7B39_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HGU2420565.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETIIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWKLLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            31699..32487
                     /gene="ycjR"
                     /locus_tag="N7B39_000525"
     CDS             31699..32487
                     /gene="ycjR"
                     /locus_tag="N7B39_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HGU2420566.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNIEEV
                     KSAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYSVENNLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLTWLRTC"
     gene            32497..33552
                     /gene="ycjS"
                     /locus_tag="N7B39_000526"
     CDS             32497..33552
                     /gene="ycjS"
                     /locus_tag="N7B39_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HGU2420567.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAREMCDTARKQGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGYPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQREMADDNRHLGSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            33549..35816
                     /gene="ycjT"
                     /locus_tag="N7B39_000527"
     CDS             33549..35816
                     /gene="ycjT"
                     /locus_tag="N7B39_000527"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HGU2420568.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSHEAWQRELDFASGELR
                     RNVVWRTSNGAGYTIASRRFVSADQLPLIALEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEICAQQSYDQLLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRSGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMARYNVQQALNIARQFGCSDDA
                     FIHRAEMFLKELWMPETQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAVSCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            35813..36472
                     /gene="pgmB"
                     /locus_tag="N7B39_000528"
     CDS             35813..36472
                     /gene="pgmB"
                     /locus_tag="N7B39_000528"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HGU2420569.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERTQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWPRLSAFWQN
                     V"
     gene            36486..37568
                     /locus_tag="N7B39_000529"
     CDS             36486..37568
                     /locus_tag="N7B39_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU2420570.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQESLH
                     KPIVMGMRPEDIHPDAQEENNISAKISVVELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            37613..38518
                     /gene="ompG"
                     /locus_tag="N7B39_000530"
     CDS             37613..38518
                     /gene="ompG"
                     /locus_tag="N7B39_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HGU2420571.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRISLAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLESD
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSISLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(38629..39627)
                     /gene="ycjW"
                     /locus_tag="N7B39_000531"
     CDS             complement(38629..39627)
                     /gene="ycjW"
                     /locus_tag="N7B39_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGU2420572.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHTPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLEDHNIAVRD
                     EWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGRQSCELLFRLIAGKPSPQNITVATHMTLK"
     gene            39782..41179
                     /gene="ycjX"
                     /locus_tag="N7B39_000532"
     CDS             39782..41179
                     /gene="ycjX"
                     /locus_tag="N7B39_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HGU2420573.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRYKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            41176..42237
                     /gene="ycjF"
                     /locus_tag="N7B39_000533"
     CDS             41176..42237
                     /gene="ycjF"
                     /locus_tag="N7B39_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HGU2420574.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            42385..43926
                     /gene="tyrR"
                     /locus_tag="N7B39_000534"
     CDS             42385..43926
                     /gene="tyrR"
                     /locus_tag="N7B39_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HGU2420575.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRASKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(43970..44476)
                     /gene="tpx"
                     /locus_tag="N7B39_000535"
     CDS             complement(43970..44476)
                     /gene="tpx"
                     /locus_tag="N7B39_000535"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HGU2420576.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            44595..45560
                     /gene="ycjG"
                     /locus_tag="N7B39_000536"
     CDS             44595..45560
                     /gene="ycjG"
                     /locus_tag="N7B39_000536"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HGU2420577.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDTTLIVDANESWHAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            complement(45535..46263)
                     /gene="mpaA"
                     /locus_tag="N7B39_000537"
     CDS             complement(45535..46263)
                     /gene="mpaA"
                     /locus_tag="N7B39_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HGU2420578.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(46554..47192)
                     /locus_tag="N7B39_000538"
     CDS             complement(46554..47192)
                     /locus_tag="N7B39_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309933.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU2420579.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSPKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            complement(47213..48143)
                     /locus_tag="N7B39_000539"
                     /pseudo
     CDS             complement(47213..48143)
                     /locus_tag="N7B39_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622627.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(48143..49063)
                     /locus_tag="N7B39_000540"
     CDS             complement(48143..49063)
                     /locus_tag="N7B39_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU2420580.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAIMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPTQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            49201..50100
                     /gene="pgrR"
                     /locus_tag="N7B39_000541"
     CDS             49201..50100
                     /gene="pgrR"
                     /locus_tag="N7B39_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HGU2420581.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLIIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            50436..52049
                     /gene="mppA"
                     /locus_tag="N7B39_000542"
     CDS             50436..52049
                     /gene="mppA"
                     /locus_tag="N7B39_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HGU2420582.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVCSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDVHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(52100..53131)
                     /gene="ynaI"
                     /locus_tag="N7B39_000543"
     CDS             complement(52100..53131)
                     /gene="ynaI"
                     /locus_tag="N7B39_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HGU2420583.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
     gene            53375..53632
                     /gene="ynaJ"
                     /locus_tag="N7B39_000544"
     CDS             53375..53632
                     /gene="ynaJ"
                     /locus_tag="N7B39_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HGU2420584.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(53682..54632)
                     /gene="uspE"
                     /locus_tag="N7B39_000545"
     CDS             complement(53682..54632)
                     /gene="uspE"
                     /locus_tag="N7B39_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HGU2420585.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(54784..55536)
                     /gene="fnr"
                     /locus_tag="N7B39_000546"
     CDS             complement(54784..55536)
                     /gene="fnr"
                     /locus_tag="N7B39_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HGU2420586.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDTLAQLAGHTRNVA"
     gene            complement(55731..56246)
                     /gene="ogt"
                     /locus_tag="N7B39_000547"
     CDS             complement(55731..56246)
                     /gene="ogt"
                     /locus_tag="N7B39_000547"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HGU2420587.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGELAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(56257..57783)
                     /gene="abgT"
                     /locus_tag="N7B39_000548"
     CDS             complement(56257..57783)
                     /gene="abgT"
                     /locus_tag="N7B39_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HGU2420588.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS
                     "
     gene            complement(57820..59265)
                     /gene="abgB"
                     /locus_tag="N7B39_000549"
     CDS             complement(57820..59265)
                     /gene="abgB"
                     /locus_tag="N7B39_000549"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HGU2420589.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSVTP
                     GENGHGCGHNLLGTAAFAAAIAIKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYQALSHFGTPEWNSEELAFAKQIQ
                     ATLTSNDRQNSLNNIAATGGENGKVFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTLNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            complement(59265..60575)
                     /gene="abgA"
                     /locus_tag="N7B39_000550"
     CDS             complement(59265..60575)
                     /gene="abgA"
                     /locus_tag="N7B39_000550"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HGU2420590.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNENSRMGLPDELTLQREFERARQQGALAQWIAAFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTTIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGYNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            60751..61659
                     /gene="abgR"
                     /locus_tag="N7B39_000551"
     CDS             60751..61659
                     /gene="abgR"
                     /locus_tag="N7B39_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU2420591.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
BASE COUNT        15311 a        15298 c        16279 g        14934 t
ORIGIN      
        1 gagaaacgta taatcagcaa aaatgagaac gatcattctc accaagtgat aggccaggag
       61 acagaaatga ccagcaagct ggaaatacgc cacaaacagc gtcaggatga aatcattaac
      121 gccgcccgtc ggtgttttcg tcgctgcggg tttcatgccg ccagtatgtc gcaaattgcc
      181 agcgaagctc agcttagcgt gggtcagata tatcgttact tcgccaacaa agacgccatt
      241 attgaagaga tggttcgccg tatcatcgac tttcgtattg cccaaatgga cattgacgcg
      301 cgtaccgatc acttcccgga agtccttgcc ttgcgtaaat ccttaaatga ggacgacgac
      361 gcgctgatgc tggaagtggc tgcagaagcc acacgcaacc cgcgggttat ggcaatgctt
      421 gaagaagctg acgccagaat gttcgctaac ggttgtgcac atatgaaaag aatgcatccc
      481 catcttagcg aggagcacat tcgttgctgc gttgaagtgt ttgctaccat gatggaaggt
      541 acggtttatc gtcgtttaac accgcaaaaa agcgatccac aacatctgca ggaaatatat
      601 caggatattg ttagtatgtt gataaataaa taagaacaga attaaccaat agcgggggat
      661 attaatcccc cttatttcat tgcgaaattc atttcgcgat gcgggttcgt tataactgaa
      721 tatccctgac atatcctcat taattttaat gaggagggta tttctcaccc tggagaaaat
      781 aatgaagtat atagcaacat ctgtagtggc aatgctgctc ttatctggtt gtgataatac
      841 gcaaagtaac aattcatccc cgtcagaaac agaagtcggc gttgttacag tcaaatctca
      901 accggtctcg gtagtcagtg aattaaccgg acgcaccagt gctgcgctca gtgccgaagt
      961 acgtccgcag gttgggggaa ttatccagaa acgcttattt aaggaaggtg atctggtcaa
     1021 ggctggacag ccgctctacc agattgatgc ggccagttat caggctgcat ggaatgaagc
     1081 tcgggcagca ttacaacaag cacaggcact ggtaaaagcc gattgccaga aagcgcagcg
     1141 ttatgcccga ctggtgaaag agaacggtgt ttcacaacag gatgctgatg atgctcagtc
     1201 tacctgtgca caagataaag ccagtgtagc ggcgaaaaaa gccgcactgg aaactgcgcg
     1261 cattaatctt gactggacca cggtaaccgc accgatttca gggcgcattg gcatttcgtc
     1321 ggtaacccct ggcgcactgg tgaccgcgtc gcaagataca gcgttaacga ctattcgtgg
     1381 tctggataca atgtatgtcg acctcactcg ctccagtgtc gatttattac gtctgcgtaa
     1441 acagtcactg gcgaccaaca gtgacaccat gagcgtctca cttattctgg aagatggcac
     1501 aacctacagc gaaaaagggc gtctggaact caccgaagtc gctgtagatg agtctaccgg
     1561 ttcggtgaca ttacgggcga ttttccccaa tccacaacag caattattac cgggaatgtt
     1621 tgttcgcgct cgtgtcgatg aaggcgtgat ggaagacgct attctcgcgc cgcaacaggg
     1681 cgtcacgcgc gatgctaaag gcaatgcaac tgcgctggtg gtgaataaag acaataaagt
     1741 agagcagcga acgctcgaaa cgggagaaac atatggtgat aaatggctgg tgctaaacgg
     1801 cctgcacagc ggcgaccgac tgattgttga aggttctgcc aaagtcactt cagggcagac
     1861 ggtcaaggct gttgaagttc aggctaatgg aggcaacgcc tgatgttttc gcgctttttt
     1921 gttcgccgcc cggtctttgc ctgggttatc gccattttga ttatgttggc ggggattctg
     1981 gctattcgca cattgcccgt cgcgcaatat cctgacgttg cgccaccgac cattaaaatt
     2041 tcagccactt atactggtgc ttctgccgaa acgctggaga acagcgtgac tcaggtcatc
     2101 gagcaacaac tcactgggct tgataattta ctctatttca gctcaaccag cagctctgat
     2161 ggttcggtca gtattaatgt gacctttgaa caaggtaccg atccagatac tgcacaggtt
     2221 caggtgcaga ataaaattca gcaggcggag tcgcgcctac ccagcgaagt gcagcaaacg
     2281 ggtgttacgg tggagaaatc acaaagcaac tttttgctga ttgctgccgt gtatgacacc
     2341 actgacaaag cctccagttc ggatatcgcc gactggttgg ttagtaacgt tcaggacccg
     2401 ctggcgcgtg ttgaaggtgt ggggagtctg caagtctttg gcgcggaata cgctatgcgc
     2461 atctggcttg acccggccaa actggcgtct tactcgctaa tgccttcaga cgtgcaaagt
     2521 gctattgaag cgcaaaacgt gcaggttact gccgggaaaa taggggcatt gccttcaccg
     2581 aatactcagc aactgaccgc aacggttcgt gcgcagtctc gcttgcagac ggtggatcag
     2641 ttcaaaaata ttatcgtgaa aagccagtca gacagcgcag ttgttcgtat aaaagatgtg
     2701 gctcgcgttg agatgggcag tgaagattat accgctatcg gcaaacttaa cggtcacccg
     2761 tctgccgggg ttgctgtaat gctttcgccc ggtgcgaatg cgctgaatac ggcgacgctg
     2821 gtcaaggata agattgccga attccagcgg aacatgccgc agggatacga catcgcttac
     2881 ccgaaagaca gcactgaatt tattaaaatc tctgtagagg acgtaattca gacgctgttt
     2941 gaagccatcg tattggtggt ttgcgtgatg tatttattcc tgcaaaacct gcgtgccaca
     3001 ctgattccgg cgttagctgt ccccgtcgtt ttgctgggca cattcggcgt tcttgcgttg
     3061 tttggctatt cgattaatac cctgacgttg tttgctatgg tgctggcgat cggcttgctg
     3121 gtggacgatg ccattgtggt agtggagaac gtcgaacgca ttatgcgtga caaagggttg
     3181 cccgcgcgtg aagccacgga aaaatcaatg ggcgagattt ctggcgcact ggttgccatt
     3241 gcgctggtgt tgtcagccgt attcctgcct atggccttct ttgggggatc tacgggggta
     3301 atttatcgtc agttttcgat aaccattatc tccgcaatgc tgctttccgt ggtggtggca
     3361 ttgaccttga ctcccgccct gtgcggttcc gtcctccagc atgttccgcc acataaaaaa
     3421 ggctttttcg gcgcatttga ccgcttttac cgccgtactg aagataaata tcagcgaggc
     3481 gtaatttatg tcctgcgccg tgctgctcga acgatggggc tttatctcgt actgggtggt
     3541 ggaatggccc tgatgatgtg gaaactgccg ggcagtttct tacccactga agaccagggc
     3601 gaaatcatgg tgcagtacac gctgccggcc ggggcgaccg ctgcccgtac agcagaagtg
     3661 aatcgccaga ttgttgactg gttcctgatt aacgagaaag caaataccga tgtcattttt
     3721 accgttgatg gtttcagttt tagcggcagc ggacagaaca ccgggatggc gtttgtttcg
     3781 ttgaaaaact ggtctcaacg taaaggggca gaaaacaccg cccaggctat cgccctacgg
     3841 gcaaccaaag agctgggcac aattcgtgat gccacggtat ttgcgatgac gccgccagcc
     3901 gttgatgggc tggggcaaag caatggtttt acgtttgaat tgttagctaa cggtggaacc
     3961 gatcgcgaaa cactactgca aatgcgtaat cagttgatag aaaaagcgaa tcaaagtccg
     4021 gagttacatt ctgttcgtgc caatgatttg ccacaaatgc cgcaattgca ggtagatatt
     4081 gatagtaata aagcggtgtc attagggttg agtttgaatg atgtcaccga caccctgtcc
     4141 agtgcgtggg gcggtactta tgtgaatgac tttattgacc gtggtcgtgt gaaaaaagtc
     4201 tacattcagg gcgacagtga atttcgttcc gcgccgtcag acttaggtaa atggtttgtg
     4261 cgcggtagtg ataacgccat gacaccattc tctgcttttg cgaccacccg ctggctgtat
     4321 ggaccggaaa gactggtgcg ctacaacggt tctgcagcct atgaaattca gggggaaaat
     4381 gcgactggct ttagttccgg cgatgcaatg acaaaaatgg aggaactggc aaacagtctt
     4441 cctgcgggaa caacctgggc ctggagtggt ttgtcattgc aggagaaact ggccagcggt
     4501 caggcattga gtctgtatgc agtttctatt ctggtggttt tcctctgcct tgcagcactg
     4561 tacgaaagct ggtcagtccc gttctcggtc attctggtga tccctctggg gcttcttggc
     4621 gcggcgctgg cggcctggat gcgtgattta aacaacgatg tttacttcca ggtggcgcta
     4681 ttaaccacta tcggcttgtc gtcgaaaaac gccattctga ttgtggaatt tgctgaagcg
     4741 gcggttgcag agggttattc cttgagtcgt gcggcattac gcgcggcgca gactcgttta
     4801 cgcccaatca tcatgacctc gctggcgttt attgcggggg gtaatgccgc tggcgatagc
     4861 aaccggctca ggggcgaaca gccgcatcgc cattggtacg ggcattattg gcggtacgct
     4921 gaccgctacg ttgctggcta ttttctttgt tcctctgttt tttgtactgg tgaagcgttt
     4981 gtttgccggt aaatcgcgcc gtcaggagta agttatgttg cgtcgttctc ttatttttct
     5041 ggtgctgttg agtgcgggat gtgtctcgct cgatccccac tacagcacgc ctgaatcccc
     5101 aatcccggcg accttgccgg gagcacaggg ccagggaaag gcaatcagcc atgactggca
     5161 gcaggtgatc cacgatcccc gattgcagca ggtggtgact attgcactga acagtaaccg
     5221 cgatgtgcaa aaagcgattg ctgatattga ctcagcacgg gcgctctatg ggcaaactaa
     5281 tgcgtcgtta ttcccgacgg tgaatgcggc cttaagttca acccgcagtc gttcgctggc
     5341 gaatggtacg gaaaccactg ccgaggctga cggcacggtg tccagtttta cgttagatct
     5401 gtttggtcgt aatcagagtt tatctcgggc ggcgcgagaa acctggcttg ccagtgaatt
     5461 tacagcccag aacacccgtt taacgctgat tgctgaaatc agcaccgcct ggctgacact
     5521 ggcggccgat aacagcaacc tggccctggc aaaagaaacg atgaccagtg cagaaaactc
     5581 attgaaaatt atccagcgcc agcaacaggt tggcacggcg gcagcgacag atgtcagtga
     5641 ggcgatgagt gtttatcagc aagcgcgcgc cagcgttgcc agctatcaga cacaagtgat
     5701 gcaggataaa aatgcgctga acttactggc aggtacaacg cttgaggaaa atttgctacc
     5761 gggaacactg gaaagcctgc cggaacagat gataagtctg gttcctgcgg gagtgtcatc
     5821 tgacgttctg ctgcgtcgcc cggatatcca ggaagcggaa cataatttga agagcgccaa
     5881 tgccgatatt ggcgcggcgc gggctaactt tttcccgacg atttcgctga ctgccagcgc
     5941 tggtgttggc agtgacgcat tgtcgtcgtt gttcagccac ggaatgcaga tctggtcatt
     6001 tacgccatcg gtcacattac cgctgttcac tggcggaagt aatctggcgc agcttcgtta
     6061 cgcagaagca caaaagcgtg gactcattgc cacctatgaa aaaaccgttc agagagcatt
     6121 taaagatgtc gctaacgcac tggcgcggcg gaccacgctt gaagagcaac tggatgcaca
     6181 gcgccagtat gttaaagctg agcaacaaac ggtcgatgtt ggcttgcgtc gctatcagac
     6241 gggtgttggt gattacctga ccgtgttgac ggcgcagcgc agcttgtgga gcgcacagca
     6301 ggagctgctg gcactgcaac tgactgattt tacaaaccga atcacgctct ggcagtcgct
     6361 gggcggcggc atgtcatcac tcaagtaagg aataatatgg ctagagtctc tctttcatgg
     6421 gcattgattc ttggtctttt agccggtatc ggcccgatgt gtaccgatct ttatttgccg
     6481 gctttgccgg agatgtctga gcaactggcg gcaaccacga ccataacgca attaacgctg
     6541 actgcatcac tgatcggtct tggcgtcgga caactgttat ttggccctct gagcgacaaa
     6601 atagggcgta aacgtccgtt aatcttgtcg ttgctattgt ttattgtttc ttccattttg
     6661 tgcgcgacaa cgaacaatat ttactggctg gtggtctggc gttttattca agggatcgcg
     6721 ggggcgggtg gttcggtgct ctctcgttct attgctcgtg acaaatatca gggagtaacg
     6781 ttgacccagt tttttgcgct gttaatgacg gtgaatggcc tggcaccggt gttgtcgcca
     6841 gtgctgggcg ggtacattgt tagcactttt gactggcgca ctttattctg ggtaatggct
     6901 gaaattagca ccgtattgtt gctgggctgc gtgttattta ttaatgagac cttgccagaa
     6961 aataaaaggg gctcatcatt gctattaacc ggacgaagcg tggtgcagaa ccgccgtttt
     7021 atgcgctttt gcctgattca aagttttatg ctggcaggct tgtttgcgta tatcggctct
     7081 tcgtcgttcg tgttgcagaa ggaatttggc tttagtccaa tgcaatttag cctggtgttt
     7141 ggccttaacg gcatcggact tatcattgct tcatggatct tttcgcgtct ggcgcgacgg
     7201 attaatgcga tgacattgtt gcgaggtggc ctgatagcgg caattttgtg tgcattgctc
     7261 acggtcttat gcgcatggac acaattgccc attccggcac tggtggcatt atttttcact
     7321 attgcatttt gtagcggcat cggcactgtt ggcggggcag aggctatgag tgcagtaggg
     7381 acgcaggaat ctggaacggc gtctgcgttg atggggatga gcatgtttgt cttcggcggt
     7441 atagccgcgc cattgtcggg atttggcgga gaaacactgt taaaaatgag tctggcaatt
     7501 acggtgtgtt atacactggc attcctggtt gctctcacca gaatcgacaa tcaaaagtaa
     7561 tgaatacctc atcctgaggg gggttcccct gtcaggatga ggtgcagaac actaacggtc
     7621 cttacgccac gcatccgccg tcaatgcttc gccaaagtgc ccggcaatca gccgtttggt
     7681 gagttcatgc agcggcgatg ccagcacatc cgcggtgctg cctcgctcga caacctcgcc
     7741 ctgatgcatc accagcacct ggtcgctaat gtgcttcatc attccgatat gctgggtaac
     7801 ataaatatac gaaatgccct gtttttcctg taattccagc atcagattaa tcaactgcga
     7861 acgcatcgac atatccagtg aagcgagggc ttcatcggca ataatgactt ttgggcgcaa
     7921 tatcagcgcg cgcgccagac ccagacgctg tttttgcccg ggggccaaca tatgcggata
     7981 gtaactgacg tgatccggta gcagcccaac catacgcatc gtttcaataa tctgtttgcg
     8041 acgctgttcc ggttccaggt tggtgttcag gcgcagtgga aaatccagaa tttgcgagat
     8101 acgttgacgg ggattcaacg aggtcgaagg atcctgaaaa atcatgcgaa tacgctgact
     8161 gcggaaggaa taatcgccaa aatgcagtgg atgatcgtca atcaataact cgccgctggt
     8221 aggttctatc attcccgcca gcatttttgc cagcgtggat ttacccgaac cattctcgcc
     8281 aataatcgcc agtgtctggc cttcacgtag cgtaaagctc aagggtttta ccgcttctac
     8341 ggtctgacga cgaaaccaac cggtccggta gcggaacgtt ttacttaaat tacgcacttc
     8401 aagcagcgtt tcgatcatct cactctttct ccatgttcag cgggaaatga caggcataga
     8461 gatgattttt cgcccccgtc aaacgtggcg tcacaatgca ttctcgttgt gcatacgggc
     8521 aacgtggccc cagacgacaa ccaatcggta actgttcaag cagtgggata gcgccgggta
     8581 gcgtattgag gcgactttta tgcggcatcg cgcttccgaa gtctggtatc gcgcggatca
     8641 gcgcctgggt ataaggatga tgtggcatcg tcactaactc cttactcggc gcggtttcca
     8701 ccgtttgacc gcagtaaagc acgttaattt tatccgccca ttggctaagc atttgtaagt
     8761 catggctgat aagcaaaata gtggtattgc tgttttggtt tagacgcgtc agcaggcgaa
     8821 agatttgcgc ctgggttgtt ggctccattg agtttgtcgg ttcgtcagca atcagcagac
     8881 gcggttgatt cgccagtgca atggctatca tcactttctg acattcacct tcggtcaact
     8941 catagggaaa actgcgcatc gcatctttat gatctttaat tcccacgcgg tgcagcagtt
     9001 caatggcacg gcgtttgcgc cagccaaaac gctgccacca acggccttta taggtccagg
     9061 ctgggatgtt ttgcatcaac tggcgaccca cacgttctga aggatctaga cacgactgcg
     9121 gttcctggaa aatcatcgac acgttgtggc caaccagttt gcgccgttcg cgtgcggaga
     9181 gacgcagcaa atcgatatca tcaaaacgca tacggtctgc agtaacacgc cagttatctt
     9241 tattcacccc acaaatcgct ttcgcaatca aacttttgcc ggaaccggat tcaccaacaa
     9301 gaccgcggat ttcaccttcg gttaaggtca tacttacccg gtcaacggct ttaacccact
     9361 catcaccggt tttaaattca atagtcaggt tacgaatatc gagtaatggc attattccac
     9421 ccccgcaata attgcacgac ggacaccgtc gccaaggagg ttaaccaaca gcacgctaat
     9481 cataattgcc gcacctggca gcatgacagt ccacggggcg acataaatca gttccagcgc
     9541 atcaccgagc atcgctcccc attcaggcga ggggagttgt gcgccaagat cgagaaagcc
     9601 cagcgcggcg atatcgagaa ttgccatcga cagtgcgcgg gtgatctcgg tgaccagccc
     9661 ggcggtgatg ttaggcatca cagcaaacca gagaatattc agcgttgatg cgccatccag
     9721 acgggcggcg ataacgtact ctttttccag ttcgtcatgc accatgctgt aaatcgaacg
     9781 taccatacgc ggcagcagcg ccagccagac agcaaacata gcgtgggaca aactcggtcc
     9841 ggcaaacgct acaacgataa ttgccaacag cagggaaggg atcgccagta aggtatccag
     9901 aatatggtta agcaccgctg agcggaggcc gtgcgtcgcc ccggcgaagg tgcccagtac
     9961 caggccacag atcgtcgcgg caagtgtcac cacaaatgcg ccacccacgg ttggcgcagc
    10021 tccgctcagt aaacggctta aaacatcgcg tccgaggtcg tcagtcccca ggaagaaaga
    10081 aacttcgcca tagcgcgacc atgatggcgg caataattgg taaccgagaa attgctggtc
    10141 gatgccgtaa ggcgcaaacc agccgccaaa catacacagt acagccagcc ccgcgcagcc
    10201 gtacaggccg accatcgcag aggcgtcact ataaaatttg cgccaggcgg tacgcagcgt
    10261 gcccggcggg cgcttttcgc tgtatacgct atcgtaaggc ataccattcc ttatgtttca
    10321 gagggttagc catggcaccc aaaatatcag aaatcacgtt aacaataatg accagtgagc
    10381 cacacaccat cactccggcg gaaatggctg cgtaatcctg ctggcggatg gcgttaatta
    10441 accagcgccc taaacccggc cagctaaaaa ccatttcggt gatcatcgcc agcgtcagca
    10501 tggtggaaaa ctgtaagccc aggcgaggaa taaccggcgg taacgcgtta tgcagaacgt
    10561 ggcgacgcaa aatagtaaag cgcgacaaac cgcgggtcgc cgccgctttt acatagttct
    10621 ggtcatacac ttcgatagtg ctgatacgca tcaggcggat cacttcagtt gttggcgcaa
    10681 ccgacagggt gatcacgggc aatatcatat ggcggattgc gctcatgatc atttcatccc
    10741 gccatggtga gtccgaaagc caggcatcaa tcaacgcaaa accggtaatc ggtttcactt
    10801 cgtagagcag atcgaaacgc cctgaaacgg gcaaccaacc tagcgtgagt gaacaaaaca
    10861 gtgtcaacag aagcgccagc cagaacaccg ggattgaaaa gcccagtaag gcgatggcgt
    10921 tgatcaagtt atcctgccac ttatggcgcg taatcccggc aatcatcccc acggggatgc
    10981 caacaatcag cgcaaaaccg aaggcgagga tgcacagttc catcgtcgcc gggaagacct
    11041 cttttaactg ttcggcaatt ggttgaccgt taatgctgga aacaccaaaa tcccagtgga
    11101 tcaggccgtt aaaccagaac acccaggcat tccacagtga cgcgccttgt aacggcgcgt
    11161 gtggagtgaa atagctcaag ctgaagccaa cgaaggtcag caggaacaag gtgacaatca
    11221 ataacaaaat gcggcgtaag gtgaagataa tcatggtttt ttcacctcat cctgtttctc
    11281 gcgatacacc ccagcaaagg aggcgttacc aaacgggcta agtaccagac ctttgatatc
    11341 gtaccgatag gcctgcaaac gcaatgacga cgccagcggc aaaatgggca attcctgcgc
    11401 cagaatactc tgcgcttcgt cataggcttc aatacgcgcc gccagctgct gcgaggagag
    11461 cgtcttacgc aacacgctgt cgaatttcgg atcgcaccag tgggcgaggt tggtctgtga
    11521 atgaattgcc gcgcagctca gtaacggacg gaagaaactg tccgggtcgt tactgtccgt
    11581 cgcccaaccg gataacgtca gatcatggct catatccatc aaccgcgcct cctgaaagcg
    11641 accttctacc ggcacaatca ccacttttac gccaacctgc gccatatccg cctgaatcag
    11701 ttcggcagtt ttcagtggac tggggttcca cgcctgcgaa cgtgtgggca cccacagttt
    11761 cagcgttaaa ttttccagcc ccaacaactt caactgttcg cgcgatttcg ccggattgta
    11821 ttcagtaatt ttagcctcgt tgtcataggc ccacgaggcg cgcggtaaaa tagaggccgc
    11881 cgtttcagcc gtaccataat agatggattg catcaggcgc tggttattaa tcgccagtgc
    11941 cagcgcatgg cggacagcgg gattatttag cggcggtttg gcggtgttaa atgccagata
    12001 ggcgacgttc atcccaggac gcagcgttaa acgcaagcgc gggtcgtcac gcaaaatgga
    12061 tagctggctg gcagcaggcc aggccagaac gtcgcattcc ccggtcagga gtttcgacag
    12121 acgtccggtg ccgccggagc ctaaatccac caccacctgc ggcattaacg gtttaccgcg
    12181 ccagaagtca tcatgacgtt gtaggcgaat aaattgcccg gcgcggtatt ccgacaactg
    12241 atacggtcca gtgccgaccg gttgacggtc gagttgctcc tggcgatctt ctttctctaa
    12301 cttccgggca tattctgccg acatgaccga agcataatgg gttgcgaggt gccacaaaaa
    12361 agaagcatcc ggctgagcca gacggaactc aacggtatga ttatccagtt tacggacgct
    12421 tttcacgtta tcggcaaatt gcaggctgtc gaagtagggg aagttgctgc cgttgacgtt
    12481 atgccacggg ttgttgcggt caaaaattcg ctggaaggta aacaccacat cgtcggcatt
    12541 cattttgcga gtgggagtaa accaggcggt tttttgaaac gggacatcgc gacgcaggtg
    12601 gaagcgatag gtcgcgccgt tgtcgagtac ttcccagctt tcggcaagtt ctggcatcag
    12661 gcgataggta tagggatcga catccagcag tcgatcataa aactgggcgg caagggtatc
    12721 gacaattaac ccactgctcg ctttggatgg gttaaaggtg ttgacttgcc cgctgacgca
    12781 atagacaaaa ccgctgtcgc ggatatcagc atgcgggggg gattcaggcg cggcgattgc
    12841 ctgaccactc acaagtccag caatcaccaa aagagacgat aatacctggc gcataatatt
    12901 aagggatttt atgtaaagag gctatcttac taatatttaa tgacattttc cattaccgtt
    12961 tgtgttcagg ggtcgtaatg agggccgtgt ggttggtcgt tgaacagatg gcgtcgccat
    13021 ctgttcaaca ttcgtacccg tgatttctct ataactataa ctcacagaac aacttagcga
    13081 ggagcagagc ggtacgatcg tgagaaaccc attaaagcac gctgcagtgc gtgtcattgt
    13141 tagccggatg cggcgtgaac gctttatccg gacaacgata ctgaccgatc gtctgcaatt
    13201 aaataattac tcattacccc attgattcaa aaactctgcg atctcatcaa tgcgtacggg
    13261 attaatcccc gcttcagcag ccatttcatg ctgggcttct tcgctgatct cttcattgtt
    13321 catcaaacgg gtgagcagta actggaagta gtgggccagc ggtgtgcgtt cagcgtctgc
    13381 gacaggctct gcgcattcgg tcgcgtactc atcggcaata tcaaaatatt ttagcgggat
    13441 atcgtggttc attatttgcc cctggggtta atgcgctaag cataagggcg gatgatagca
    13501 ctcgtgatcc gggtcataca tctttttata gttaatggcg cggcgcatta ccctcaggca
    13561 ggataatgcg gcgcgcatcc gactattacg tttcgctcac tggcgttctg tcaccttcgt
    13621 acagcggcac tttgcgatag cggcggatca aataccacaa atatgcgccg cctaacgaag
    13681 cccataccag accgagtgtc agtgacgttg actcgaggtt aacccacagc acacccaccg
    13741 tcagcgcgcc aaccagcggc atcagcaaat agtggaagtg atccttccag cttttattca
    13801 ttcctttacg ccgccagaaa tgattaaaca ccgacagatt cacgaaggta aacgccacca
    13861 gagcaccgaa gttaatcaat gctgtcgcgg tgactaaatc aaagaacaat gccgacatcg
    13921 cgacaattcc gaccataatg acgttcagtg ccggagtccg ccattttggg tgcacatagc
    13981 caaacacgcg ctccggaaac acattgtcgc gccccatcac atacagcaga cgtgacacgc
    14041 tggcatgtga ggccaggcca gacgctaacg tgttcacaaa cgtggtgcag aggaaaatcg
    14101 actggaacag cttaccgccg acgtagagtg caatttcagg cagtgcggcg tccgggtctt
    14161 taaagcggct gatatcgggg aagaacagct gcataaagaa cgacgccgcg ataaagataa
    14221 cgccaccata gaccgccgtc aggaagatgg ctttcgggat cacgcgtgcg gcgtctggtg
    14281 tctcttccga aagtgtggtc accgcatcga aaccgaggaa cgagaaacag acaatcgtcg
    14341 ccccggtaat aatcgggatc aggtgcgcgt tctcgctgat aaacggctga agtgaccaga
    14401 cggtgccaac gccttctcct ttatgcagtc cctgaaccac caggaagata aacaccacca
    14461 tgatggagat ttgcaccagt acaaacaggg tattgaagtt agcgaccagg ttgacgctct
    14521 tcagattcgc ggcggttaaa atggcgacga aggttaccac ccacacccac ggcggcactt
    14581 ccgggaagag ggcggagaga tagattttcg ccaacaaaac gttgatcatc ggcaaaaaga
    14641 gataatccag cagtgatgac cagccgacca taaatccgac gtgcgggtta atcgactttt
    14701 gcgcgtaggt ataggccgaa ccggcctccg gaaactggcg aaccagtttg ccgtagctga
    14761 tagcggtaaa cagcacgccc gccagcgcca gcaaatagga cgcagggacg tgaccgtcgc
    14821 taatgccaga tacaatgcca aaggtatcga acacagtcat cggcgtgaga taggccagac
    14881 ccatcatcac aacctgccac aatttcagtg attttcgcag acgggttttg ccgggttgcg
    14941 cagcaatatt cagtggtgaa ttaatagcca taattgctgc ctcccctgcg taataaggtt
    15001 tgcggtcgtg tcgacctgaa atcaaacctg tttagcaagg gaagcaaagg gggcggagtc
    15061 cagtcagtcg caggaggggc gcatacgcag cctcccgcgc catgcgggga tttaatcatc
    15121 ggcatcatct catttcctcg tcacagtctt aatcgttaac ggaagcccgt tgctgcctgc
    15181 gctccataac gcgacatccg ggaaatgtct ggattaccag caaatgtcag gcctggctcc
    15241 gctcaggccg atgaaacaac cccgcaaggg gtactatgcg tttttcaaca tccactcaat
    15301 ttctgtttct gtgatgaggc gctcaaactg caacagctca tcatttttac aggcgtgata
    15361 cacatggcaa aaccgttcgc ctaaatagcg gcgcaggtga tcattctcga taaactcacc
    15421 cagggcatcg ctctggcgaa tcgggaaggg taagccttcc tgttccagcc cgttaccttc
    15481 gacttcttcc tgcaacggca gctcgttatc aaggccatgc aaaataccgg caaaaatcgc
    15541 tgccatcacc agatatgggt tggcatcggc acccgccacg cgatattcca cgcggtgatt
    15601 atgacgatcg ccgcatggaa tacgcagggc gacggtacgg ttattatggc cccacgacgc
    15661 ctgcgtcggt acatacatcc ccggctggaa gcggcgatac gagttcacgt ttggtgccag
    15721 caacgccatt gatgacggca tcaggtcaat catcccggcg agcatctttt tcagcagcgg
    15781 cgaatcttcg ccttctgtgt cagaaagcac gttctcgcca cgattatttt gcatactgat
    15841 atggatatgc attccgctgc ccgcgtgctc ttcatacggc ttcgccataa aagtggcgtg
    15901 catcttatgc ttttctgcca tcagacgcac caggcgtttt agtgccagcg catcatcgca
    15961 ggcttccagc acgttatcag tatggtaaag gttgatttca aactgacccg gcgaggcctc
    16021 agcgaccgcg ccatctgccg gaatcagctg taactgcgcc agttcatcaa tatcattgag
    16081 cacgtcggcg aagtggttga ggttatcaac ggagtaaacc tggctttgtg tattgcggtc
    16141 atcagtgccg ggcgcgcagg gcggttgcag atacccttca gcgtcgcgct ggcgatccag
    16201 taaatagaac tccagctcta ccgctacgac cgggaacaat ccgcgctggc gcagctgctg
    16261 ccacaggcgg ttaagaacgt tccgcggctc aacgtcaaag ggagcgccat cttcatcgac
    16321 catggtcagg agcatctgac cgataaactc tggatcggcg gcagaagggg ttaaggaacc
    16381 gagaacagga acacaggtac gatccggctc gcccatttcc tgacccagac ctgcttcttc
    16441 tactacgttg cccagaatat ccattgcaaa taccgaggcc gggaaataac accctttctc
    16501 gagcttcttc aggcttgaaa caggaatacg ttttccacgg aagcaaccat tcaaatcggt
    16561 aagtaaaaca tcaacatatt gcgtattcgg gtagcgctcc aggtagcgtt tcacttcctg
    16621 cgtaaaggcg ctaccccgcc tctcttctga ctgctgaaca aagttctcta cttcaacgat
    16681 attggtttcc atgattcttc gcctttggtt tgttttccgc tcgttatcaa agcgtaaaat
    16741 ataatgacca ccattcgaat ctgtatgcaa actaaatgtt tgtcaaatgt taaattgagt
    16801 ttgcaaaaat gaaaacccac tgctagattg aaaaaatatt gaacataaag gtcatttaaa
    16861 ccgcagtaat ggcgataatt tagtccactt tgtgagattg agaatggaaa atataatgaa
    16921 caatccggtt atcggtgtcg taatgtgcag gaacaggctt aaggggcatg cgacccagac
    16981 tctgcaagaa aagtacctga atgccatcat ccacgcaggc ggcttgccaa ttgcgctgcc
    17041 acatgcgctg gcggaaccgt cgttacttga acaacttttg ccgaaactcg atggcattta
    17101 ccttcctggt agtcccagca atgtgcagcc gcacctatat ggtgaaaacg gcgatgagcc
    17161 tgacgccgat cccgagcgtg atcttctgag catggcgtta attaatgccg cactcgaaag
    17221 gcgcatcccc attttcgcca tctgccgggg tttacaggaa ctggtggtgg caaccggtgg
    17281 ttcgttgcat cgcaagctgt gcgaacagcc tgaattgctg gaacatcggg aagatcccga
    17341 actgccggtg gaacagcaat atgcaccgtc gcatgaagtt caggttgaag aggggggatt
    17401 gctgtctgca ttgttacctg aatgtagcaa cttttgggta aactctctgc atggacaagg
    17461 ggcgaaggtc gttagcccac ggttgcgtgt tgaagctcgc tcgccggatg gcttagttga
    17521 ggcggttagt gtcatcaatc atccttttgc gctgggcgta cagtggcacc cggaatggaa
    17581 cagtagcgag tacgcgcttt cgcgtatatt gttcgagggc tttatcaccg cttgtcagca
    17641 ccatatcgct gaaaaacagc gactctgacc actacagttt aaggaaatgc aaatatgagt
    17701 gatgagggac tggcgccagg aaaacgcttg tcggaaatcc gccagcagca ggggctttca
    17761 caacgtcgtg ccgccgaact ctccgggctg actcacagtg ctatcagtac gatagaacaa
    17821 gataaagtca gccctgccat cagtacgctg caaaagctgc tgaaggtgta tggtctgtca
    17881 ctctcggaat tcttttccga gccggaaaaa cctgatgagc cgcaggtcgt cattaatcag
    17941 gacgatttaa ttgagatggg cagtcagggt gtgtcaatga agctggttca taacggtaac
    18001 ccgaatcgca cgctggcgat gatctttgaa acgtaccagc cgggcacaac cactggggaa
    18061 agaattaagc atcagggtga ggaaataggc actgtactgg aaggtgaaat tattctgacg
    18121 attaatggtc aggattacca cctcgtcgcg gggcaaagtt atgccattaa taccggcatc
    18181 ccgcacagtt tcagtaatac gtcggcaggt atttgccgaa ttatcagcgc ccatacgccc
    18241 accacgtttt aatctttttg ttctgtaagc cgggtaagcg caatgcgccc ggcaatccat
    18301 aaagaatgtc gccagaaata aacgggcata cggcccgggg atctctgcgc cctgacgttc
    18361 acaaactgca tatatctgat agacgtgaaa caggagtcat aatgaatttt catcatctgg
    18421 cttactggca ggataaagcg ttaagtctcg ccattgaaaa ccgcttattt attaacggtg
    18481 aatatactgc tgcggcggaa aatgaaacct ttgaaaccgt tgatccggtt acccaggcac
    18541 cgctggcgaa tattgcgcgc ggcaagagcg tcgatatcga ccgtgcggtg agcgcagcac
    18601 gcggcgtatt tgaacgcggc gactggtcac tctcttctcc ggcaaaacgt aaagcggtgc
    18661 tgaataaact cgccgattta atagaagcca acgccgaaga gctggcactg ctggaaactc
    18721 tcgacaccgg caaaccgatt cgtcacagtc tgcgtgatga tatacccggc gcggcgcgcg
    18781 ccattcgctg gtacgccgaa gcgatcgaca aagtgtatgg cgaagtggcg accaccagta
    18841 gccatgagct ggcgatgatc gtgcgtgaac cggtcggcgt gattgccgcc atcgtgccgt
    18901 ggaacttccc gctgctgctg acttgctgga agcttggccc ggcgctggca gcagggaaca
    18961 gcgttgtcct gaaaccgtct gaaaaatcac cgctcagtgc gattcgtctc gcggggctgg
    19021 cgaaagaagc aggcttgccg gatggtgtgt tgaacgtggt gacgggtttt ggtcatgaag
    19081 cggggcaggc gctgtcgcgt cataacgata tcgacgccat tgcctttacc ggttcgaccc
    19141 gtaccgggaa acagctgctg aaagatgcag gcgacagcaa catgaaacgc gtctggctgg
    19201 aagcgggcgg caaaagcgcc aacatcgttt tcgctgactg cccggatttg caacaggcgg
    19261 caagcgccac cgcagcaggc atcttctaca accaggggca ggtgtgcatc gccggaacgc
    19321 gcctgttgct ggaagagagc atagccgatg aattcttagc cctgttaaaa cagcaggcgc
    19381 aaaactggca gccgggccat ccacttgatc ccgcaaccac catgggcacc ttaatcgact
    19441 gcgcccacgc cgactcggtc catagcttta ttcaggaagg cgaaagcaaa gggcaactgt
    19501 tgttggatgg ccgtaacgcc gggctggctg tcgccatcgg cccgaccatc attgtggatg
    19561 tagacccgaa tgcgtcctta agccgcgaag agattttcgg tccggtgctg gtggtcacgc
    19621 gtttcacatc agaagaacag gcgctacagc ttgccaacga cagccagtac ggccttggcg
    19681 cggcggtatg gacgcgcgac ctctcccgcg cgcaccgcat gagccgacgc ctgaaagccg
    19741 gttccgtctt cgtcaataac tacaacgacg gcgatatgac cgtgccgttt ggcggctata
    19801 agcagagcgg caacggtcgc gacaaatccc tgcatgccct tgaaaaattc accgaactga
    19861 aaaccatctg gataagcctg gaggcctgaa atgacagaac ataccagcag ttactacgcc
    19921 gccagtgcga ataaatacgc gccattcgac acgctgaatg agtcaatttc ctgcgacgtt
    19981 tgcgtggttg gcggcggcta taccgggctg tcttccgcac tgcatctggc ggaagcgggc
    20041 tttgacgtgg tggttctgga agcctcacgc atcggctttg gcgccagcgg gcgcaacggc
    20101 gggcagcttg tgaactccta cagccgcgac atcgacgtta tcgaaaaaag ctacggcatg
    20161 gacaccgccc gtatgctcgg cagcatgatg ttcgaaggtg gtgagatcat ccgcgaacgt
    20221 atcaaacgtt atcagattga ctgcgactac cgccccggcg gtctgtttgt ggcgatgaat
    20281 gataaacagc tcgccacact tgaagagcag aaagagaact gggaacgcta cggcaataaa
    20341 cagctggaga tgctggacgc caacgccatt cgccgcgagg tcgccagcga tcgctacact
    20401 ggtgcgctgc tggatcacag cggcgggcat attcacccgc tgaacctcgc cattggcgaa
    20461 gcggacgcta tccgcctgaa cggcgggcgc gtgtatgagc tttccgcagt gacgcagatc
    20521 cagcacacca cgcccgccgt agtgcgtaca gccacaggtc aggtgacggc gaagtatgtg
    20581 attgtcgccg ggaatgcgta cctgggcgat aaagtggagc cggaactggc gaaacgcagc
    20641 atgccgtgcg gtacgcaggt gatcaccacc gagcggcttt ccgatgattt agcccgttcg
    20701 ctgatcccga aaaactactg cgtggaagac tgtaattatc tgcttgatta ctaccgtctt
    20761 accgccgaca accgtttgct gtacggcggc ggcgtggtgt acggcgcgcg cgacccggat
    20821 gacgttgagc gtcttgtggt gccgaaactg ctgaaaacct tcccgcagtt gaagggcgtg
    20881 aaaatcgact atcgctggac gggcaacttc ctgctgaccc tgtcgcgtat gccgcagttc
    20941 ggtcgcctcg ataccaacat ctattacatg cagggttaca gcggccacgg cgtcacctgt
    21001 actcatctcg ccggacgttt gattgccgaa ctgctgcgcg gcgacgccga acgtttcgat
    21061 gccttcgcca atctgccgca ttacccgttc cccggcgggc gcacgctgcg cgtaccgttt
    21121 accgcgatgg gcgcggcgta ttacagcctg cgcgatcgtc tgggcgttta atttccgatt
    21181 aacagtgaag agtcaaaagg tgtgaaacat gagcaacaat gaattccatc agcgtcgtct
    21241 ttctgccacc ccgcgcgggg ttggcgtgat gtgtaacttc ttcgcccagt cggctgaaaa
    21301 cgccacgctg aaggatgttg agggcaacga gtacatcgat ttcgccgcag gcattgcggt
    21361 gctgaatacc ggacatcgcc accctgatct ggtcgcggcg gtggagcagc agctgcaaca
    21421 gtttacccac accgcgtatc agattgtgcc atatgaaagc tacgtcaccc tggcggagaa
    21481 aatcaacgcc cttgccccgg tgagcgggca ggccaaaacc gcgttcttca ccaccggtgc
    21541 ggaagcggtg gaaaacgcgg tgaaaatcgc ccgcgcccat accggacgcc ctggcgtgat
    21601 tgcgtttagc ggcggcttcc acggtcgtac gtatatgact atggcgttaa ctggaaaggt
    21661 cgcgccgtac aaaatcggct tcggcccgtt ccctggttcg gtgtatcacg taccttatcc
    21721 gtcagattta cacggcattt caacacagga ctccctcgac gccatcgaac gcttgtttaa
    21781 atcagacatc gaagcgaagc aggtcgcggc cattattttc gaaccggtgc agggcgaggg
    21841 cggtttcaac gttgcgccaa aagagctggt tgccgccatt cgccgcctgt gcgacgagca
    21901 cggtattgtg atgattgccg atgaagtgca aagcggcttt gcgcgtaccg gcaaactgtt
    21961 tgcgatggat cattacgccg ataagccgga cttaatgacg atggcgaaaa gcctcgcggg
    22021 cgggatgccg ctttcgggtg tggtcggtaa cgcgaatatt atggacgcgc ccgcgccggg
    22081 cgggcttggc ggcacctacg ccgggaatcc gctggcggtg gctgccgcgc acgcggtgct
    22141 caacattatc gacaaagagt cactctgtga gcgcgccatg cagttggggc agcgtctgac
    22201 gaaaaccctg attgatgcca aagaaaacgt tccggccatc gcggcggtac gtggtctggg
    22261 ctcgatgatt gcggcagaat ttaacgaccc gcaaacgggc gagccgtcag ccgccattgc
    22321 gcaaaaaatt cagcaacgcg cgctggcgca gggactgctt ctactgacct gtggcgcata
    22381 cggcaacgtg attcgtttcc tgtatccgct gaccatcccg gatgcgcaat tcgatgcggc
    22441 aatgaaaatt ttgcaggatg cgctgagaga ttaatacgca gaagcagggg caacgcaata
    22501 atgcctgata tgctgcgctt atcagacctg caaacgacgt attgattatg tatgccggat
    22561 aaggcgttca cgccgcatcc ggcaagttgt attgcccaac ttcgctaaat ctggtgcttt
    22621 ttcaacaacg cgcggaactg atgataggtt aagcccagca attcagctgc gcgcttctgg
    22681 ttatatttcc cttgttgcaa actgagttgc agcaactctt tttcctgctg catctgaaac
    22741 tcacgtaaat ccagcggcag tgttggaagc gaggtggttt ctgaaacggc gatagcgtct
    22801 tcaggcggac gccgtttaaa gggatcaata atgatgtcat caagcggata atcgctggtg
    22861 ccgtggcgat acactgaacg ttccaccacg tttttcaatt cacgaatatt tcctggccaa
    22921 cgataattca gcaatgtttc tctggcgcgc tccgtaaacc ccgggaacag aggcagcttg
    22981 atttcccgac acatctggat ggcaaagtgt tccgccatta acataatgtc gctttcacgt
    23041 tcgcgtagtg gcggcagttg cactacatcg aaagccagcc ggtcgagcag gtcagcgcga
    23101 aaagtacctt cattgaccat cgccgggaga tcggcattcg tcgcgcatac caaccgcaca
    23161 ttcacctgca acggctggct gccgccaacg cgctccagtt caccgtactc aatcacgcgc
    23221 aataatttct cctgcaccat catcggtgcc gtagcgagtt catcaagaaa tagcgtaccg
    23281 ccatcggcac gttcaaatct ccctggatga cgtttttgcg caccggtaaa cgcccccgct
    23341 tcgtgaccaa acagttcgga atccagcaga ttttcattta acgccgcgca gttaagggaa
    23401 ataaacggcc cttgccaacg ggaggagaga taatgcaggc ggctggcgat cagctcttta
    23461 ccggtgccgc gttcgccgat gatgagcacc ggtttgtcca gcggtgcgag atgcgaaacc
    23521 tgttccagca cttcgagaaa gctgttcgcc tcaccgagta aattatcttt gtattctgcc
    23581 atgatgaaat tcgccacttg ttagtgtaat tagctaattc atcctggcat gttgctgttg
    23641 attcttcaat cagatcttta taaatcaaaa agataaaaaa ttggcacgca aattgtatta
    23701 acagttcagc aggacaatcc tgaatgcaga aatcaagagg acaacattat gggtattttt
    23761 tctcgctttg ccgacatcgt gaatgccaac atcaacgctc tgttagagaa agcggaagat
    23821 ccacagaaac tggtgcgtct gatgatccag gagatggaag atacattggt tgaagtacgt
    23881 tctacttcgg cgcgtgcgct ggcagaaaag aaacagctga ctcgccgtat tgaacaagcg
    23941 tcggcgcgtg aagttgaatg gcaggaaaaa gccgaactgg cgctgctgaa agagagagag
    24001 gatttggcgc gtgcggcgtt aattgaaaaa cagaaactga ccgatctgat taagtccctg
    24061 gaacatgaag tgacgctggt ggacgatacg ctggcacgca tgaagaaaga gattggcgag
    24121 ctggaaaaca aattgagcga aacacgcgct cgccagcagg cattgatgtt acgccatcag
    24181 gcggcaaact cgtcgcgcga tgtgcgtcgt cagctggaca gtggcaaact ggatgaagca
    24241 atggctcgtt tcgaatcttt cgaacgtcgt attgaccaga tggaagcgga agcagaaagc
    24301 cacagcttcg gtaaacaaaa atcgctggac gatcagtttg ccgaactgaa agccgatgat
    24361 gcaatcagcg agcaactggc acaattaaaa gccaaaatga agcaagacaa tcaataataa
    24421 catccaggcg gcgtccgaac gcgccgccgc tcatcgtcta aggagtactt atgagcgcgc
    24481 tatttctggc tattccatta accatttttg tgctgtttgt tttaccgatc tggttatggc
    24541 tgcattacag caatcgttct ggtcgcagtg aattgtcgca aagtgagcag cagcgattag
    24601 cgcaactggc tgatgaagca aaacggatgc gcgaacgtat tcaggcgctg gaatctattc
    24661 ttgatgcaga acatccgaac tggagggatc gctaatggcg ggcattaatc tcaataaaaa
    24721 attatggcgt attccacagc agggcatggt ccgtggcgtc tgcgccggga ttgccaacta
    24781 ttttgatgta ccggtaaaac tggtgcgtat cctggtggtg ttgtcgattt tcttcggtct
    24841 ggcgctgttt accctggttg cttacatcat tttgtcattt gcgcttgatc caatgccgga
    24901 caacatggcc tttggtgagc agctaccttc cagcagtgaa ttgctggatg aagtcgaccg
    24961 tgaactggcg gcaagtgaaa cgcgtttacg cgagatggaa cgttatgtca cttccgatac
    25021 attcacgtta cgtagccgtt tccgtcaact gtgaggaaag ttatgaatac tcgctggcaa
    25081 caggccgggc aaaaggtaaa gcctggtttc aaattagcag gcaagctggt acttcttacc
    25141 gcactgcgct atggcccagc gggtgtggcg ggctgggcga taaaatcagt tgctcgccga
    25201 ccgctgaaaa tgttgctggc tgtggcgctg gaaccgctgt taagtcgggc tgccaataaa
    25261 ctggcgcagc gttataaaag gtgaggggag tttcgcaaaa attgttaaat ctcaggcgta
    25321 taatggatag caatattcat ccatagaagg acgcttacat gtttaaaaaa ggcttacttg
    25381 ctctggcact ggtgttttca ctgcccgttt tcgccgctga acactggatc gatgttcgtg
    25441 ttccagagca gtatcagcaa gagcacgttc agggggccat caatattccc ctgaaggaag
    25501 tgaaagagcg cattgccacc gtcgttccgg ataaaaacga caccgtgaaa gtgtattgca
    25561 atgccggacg ccaatcaggg caggcaaaag agatccttag cgagatggga tatacccacg
    25621 ttgagaacgc cggtggcctg aaagacatcg caatgccgaa ggtcaaaggt taaaagaatt
    25681 caccatgagc ggcgcttatg cgccgttttt ttctgtcaca ccttatttac atccctatag
    25741 attatttgcg tcagctcaca aatacgcttt ttccctggta aaaaatgatt tcctgcgtga
    25801 ctaaaaccct tgtgctcaat tgacagttta ttttctgcgg agtagtctct catttcatgg
    25861 gaccgctacc aggaaaggca acatgaaaca gaaaattacg gattacctgg acgaaatcta
    25921 cggtggaaca tttaccgcaa ctcatttaca gaaacttgta acgcgtcttg agagtgcgaa
    25981 acgattaatt acacagcgac gtaaaaaaca ctgggatgaa agtgatgtcg tgttaattac
    26041 ctatgccgat caatttcaca gtaatgattt aaaaccacta cccacattta atcagtttta
    26101 ccatcaatgg ctgcaaagca ttttttcaca tgttcattta ttaccttttt atccatggtc
    26161 atctgatgat ggattttcgg taattgatta tcatcaggtc gccagtgaag cgggtagtgg
    26221 caggatattc agcagctcgg tgaatgcagt catttaatgt ttgattttgt ctgcaaccat
    26281 atgtcggcaa aaagtgaatg gtttaaaaac tatttacaac agcagccagg ttttgaagat
    26341 ttttttattg cggttgatcc gcaaaccgat ctcagcgctg tcacccgtcc gcgtgcatta
    26401 ccgctattaa cgccgtttca aatggatgat aattccacgc gccatttatg gaccaccttt
    26461 agcgacgatc aaattgacct gaattaccgt agccctgacg tgttgctggc gatggtggat
    26521 gttttgcttt gttacctgga aaagggcgca gaatatgtcc gtctggatgc cgttggcttt
    26581 atgtggaaag agccgggaac aagctgcatc catctggaaa aaacacatct gattatcaaa
    26641 ctgttacggt cgattattga tgacatcgcg ccaggtacgg tgattattac tgagacgaat
    26701 gttccgcata aagataacat tgcttacttt ggcgaaggcg atgacgaagc gcatatggtg
    26761 taccagttct cgctgccgcc gctggtgtta catgcggtgc aaaaacagaa cgtcgaggcg
    26821 ctgtgccagt gggcgcaaag tctgagcttg ccctccggta aaacgacctg gtttaacttc
    26881 ctcgcctctc acgatggcat cgggctgaac ccactgcggg gcttgttacc agaaagcgaa
    26941 atattagcgc tggtcgaggc attacagcag gaaggtgcat tagtaaactg gaaaaataat
    27001 cccgacggta cgcgcagtcc ctatgaaata aatgtcacct atatggatgc gttaagccgc
    27061 cgcgagagta gcgatgaaga acgttgcgcc aggtttatcc ttgcccatgc gattttgtta
    27121 agtttccccg gtgtgccagc gatatatatt caaagtattc tgggctcgcg taatgattac
    27181 gcaggtgtcg aaaaactcgg atataaccgt gcgattaacc gtaaaaaata tcacagtgaa
    27241 gagataaccc gagaactgaa cgatgaagct acattaaggc atgcggtata tcatgagttg
    27301 tcgcgtttaa ttacacttcg tcgcagccat aacgagtttc atccggataa taattttacc
    27361 atcgatacgg ttaattcatc cgtaatgcgt attcaaagaa gtaacgcaga gggtaattgt
    27421 ctgaccggat tgtttaatgt cagtaaaaat attcagcatg taaatattac tggcctgcac
    27481 ggtcgggatc tgattagtga agttgatata ttgggtaata aaataacgct gcgcccctgg
    27541 caggttatgt ggattaaata aaaaggaaca tctcatgatt aaatcaaaaa tcgtgctgtt
    27601 atcagcactg gtttcctgcg ccctgatttc aggctgtaaa gaagaaaata aaccccatgt
    27661 atccatcgaa tttatgcatt cttcggtgga gcaggagcgt caggcagtta tcagcaaatt
    27721 gattgcccgt tttgaaaaag aaaacccagg tatcaccgtt aaacaagtgc ccgtcgaaga
    27781 ggacgcttat aacaccaaag tcatcactct ttcacgtagc ggctcgctgc cggaagtgat
    27841 tgaaaccagt catgactacg ccaaagttat ggacaaagag cagcttatcg atcgcaaggc
    27901 ggttgccacg gtcatcagca acgttggtga aggcgcgttt tacgatggcg tactgcgtat
    27961 tgtgcgtacc gaagatggta gcgcatggac cggtgttcct gtcagcgcct ggattggcgg
    28021 catctggtat cgcaaagatg tgctggcaaa agcggggctg gaggagccga aaaactggca
    28081 acagctgctg gacgttgcac agaaactgaa tgacccggcg aataaaaaat acggtattgc
    28141 gctgcctacg gcagaaagcg tgctgacgga acaatccttc tcccagtttg cgttatccaa
    28201 ctaggccaac gtctttaacg ccgaaggcaa aatcaccctt gattcaccag agatgatgca
    28261 ggcgctgacc tattaccgcg accttgctgc caacaccatg ccgggttcta acgacatcat
    28321 ggaagtgaaa gacgccttta tgaacggcac cgcgccgatg gcgatttact ccacctatat
    28381 ccttccggct gtgattaaag aaggcgatcc gaaaaacgtc ggtttcgtgg tgccaaccga
    28441 gaaaaactct gccgtctacg gcatgttgac ctcgctgacc attaccgccg gacaaaagac
    28501 tgaagaaacg gaagcggctg aaaaatttgt cacctttatg gagcaggcag acaacattgc
    28561 cgactgggtg atgatgtcgc caggcgcggc gctgccggtg aacaaagcgg ttgtcactac
    28621 ttccacctgg aaagacaacg acgttattaa ggcgctgggt gaactgccaa atcagttaat
    28681 cggtgaactg ccgaatattc aggtctttgg cgcagtaggg gataaaaaat ttacccgcat
    28741 gggcgatgtg acggggtctg gggtggtgag ctccatggtg cataacgtca ccgtaggtaa
    28801 agccgatctc cctggcacgc tgcaaacgag ccagaaaaag ctggatgaac tgatcgaaca
    28861 gcactaaacc caggacagga atccgcaatg aacaggcttt tttcaggtcg ttccgatatg
    28921 ccctttgcgc tgttgcttct cgcacccagc ttattactgc tgggcggtct ggttgcgtgg
    28981 ccaatggtgt cgaatatcga aattagtttt ttacgtctgc cgctcaatcc caacatcgag
    29041 tcaacgtttg ttggggtaag caactatgtg cgtattctct ccgatcccgg cttctggcac
    29101 tcgctgtgga tgacggtctg gtataccgcg ttggtggtgg cgggcagcac cgtgctcggg
    29161 ctggcagtgg cgatgttttt caaccgcgag ttccgcctgc gcaaaaccgc gcgttcgctg
    29221 gtgatcctct cctacgtaac gccgtctatt tcgctggtgt tcgcctggaa atacatgttc
    29281 aacaacggct acggcattgt gaactacctc ggcgtcgatc ttctgcatct ttatgagcag
    29341 gcaccgctgt ggttcgataa tccgggcagt agttttgtgc tggtggtgat cttcgccatc
    29401 tggcgctact tcccgtatgc ctttatctcg tttctggcga ttttgcagac catcgacaaa
    29461 tcgctgtacg aagcggcaga gatggatggc gctaatgcct ggcaacggtt tcgtatcgtc
    29521 acgctgcccg cgattatgcc ggtcctggcg acggtggtga cccttcgcac catctggatg
    29581 ttctacatgt tcgcggatgt ttatctgctg acaaccaagg tcgatattct tggtgtctat
    29641 ctctacaaaa ccgcctttgc ctttaatgac ttagggaaag cggcggcgat ctcggtggtg
    29701 ctcttcatca ttattttcgc tgtcattctg ctgaccagga aacgggtgaa cctcaatggc
    29761 aacaaataaa cgcacactca gtcgtatcgg tttttaccgc gggctggcgc tgtttctcat
    29821 catcacgctg tttccgtttt ttgtgatgct gatgacctcg ttcaagagcg caaaagaggc
    29881 gatctcgctg catcctacgc tgctgccgca acagtggacg ctggagcatt atgtcgacat
    29941 ttttaacccg gtgattttcc cgtttgtcga ctacttccgt aacagcatgg tggtgtcggt
    30001 ggtttcatcc gtggtggcgg tatttctcgg cattctcgga gcttacgcgc tttctcgcct
    30061 gcgctttaaa ggccggatga ccatcaacgc cagcttttac acggtgtata tgttctccgg
    30121 cattttgctg gtggtgccgc tgttcaaaat tatcaccgca cttggcattt acgacaccga
    30181 gatggcgctg atcattacca tggtgacgca aactctgccc accgccgtgt ttatgctgaa
    30241 aagttacttc gacaccatcc ctgatgagat cgaagaagcg gcgatgatgg atggcctcaa
    30301 ccgtctgcaa attatcttcc gtatcaccgt gcccctggcg atgtccgggc tgatatccgt
    30361 attcgtctac tgctttatgg tggcatggaa cgactatctg tttgcgtcga ttttcctctc
    30421 cagcgccagc aatttcacct taccagtcgg cctgaacgcg ctgttcagta cgccagatta
    30481 catctgggga cggatgatgg cggcctcgct ggtgaccgca ttgccggtgg tgattatgta
    30541 tgcgctttcg gaacgtttta ttaagagtgg tttgaccgcc ggtggcgtga agggctaaag
    30601 cggccagttt ctttacaagg agttttacat gaaaaagtta gtagccacag caccgcgcgt
    30661 tgctgcgctg gttgagtatg aagatcgggc gattttagcc aatgaagtga agatccgcgt
    30721 gcgtttcggt gcaccgaaac acggaacgga agtggtcgac ttccgcgccg ccagcccgtt
    30781 tatcgatgaa gactttaacg gcgaatggca gatgttcacc ccgcgtccgg cagatgcgcc
    30841 gcgcggcatt gagtttggca aattccagct tggcaacatg gtggttggcg acattatcga
    30901 gtgcggcagc gacgttaccg actacgcggt gggcgacagc gtatgcggct acggcccgct
    30961 ctccgagacg atcatcatta acgcagtgaa taactacaag ctgcgcaaaa tgccggaagg
    31021 cagctcctgg aaaaacgctg tctgctacga cccggcgcag tttgccatga gtggcgttcg
    31081 cgatgccaac gtacgcgtag gggattttgt agtggtggta gggcttggcg cgatcggtca
    31141 aattgccatc caactggcta aacgcgctgg cgcgtcggtg gtaattggcg tcgatcctat
    31201 tgcccatcgt tgtgatattg ctcgtcgtca cggtgcggat ttctgcctta atcccattgg
    31261 cactgacgta ggcaaagaga tcaaaacgct gaccggcaag cagggtgccg atgtgattat
    31321 cgaaaccagc ggttacgccg acgcgctgca gtcggcgctg cgcggcctgg cttacggtgg
    31381 caccatctcc tatgtcgcgt ttgctaaacc gtttgctgaa ggttttaacc tcggacgcga
    31441 agcgcatttc aataacgcca aaattgtctt ctctcgcgcg tgcagtgaac cgaacccgga
    31501 ttatccgcgc tggagccgta agcgtattga agaaacctgc tggaaactgc tgatgaacgg
    31561 ttatctcaat tgcgaagatt taatcgaccc ggtagtgacc tttgccaaca gcccggaaag
    31621 ctacatgcag tatgtcgatc agcatccgga acagagcatc aaaatgggcg tcacgtttta
    31681 attcaaagga atgaaaaaat gaaaataggc acacagaatc aggcgttctt cccggaaaat
    31741 attctggaga aatttcgtta tatcaaagag atgggcttcg atggttttga gattgacggc
    31801 aaactgctgg tcaacaacat cgaagaagtc aaatcggcga tcaaagaaac cggtttaccg
    31861 gtgaccaccg cctgcggcgg ctatgacggc tggattggtg actttatcga agagcgtcgt
    31921 cttaatggct taaagcagat cgaacgcatt ctcgaagcgc tggcagaagt ggggggtaaa
    31981 ggtatcgtcg ttccggcagc gtggggcatg tttaccttcc gcttaccgcc gatgacctcg
    32041 ccgcgtagcc tggacggcga ccgcaaaatg gtgagtgatt ccctgcgcgt actggaacag
    32101 gtcgccgcgc gtaccggaac cgtggtgtat ctcgaaccgt taaaccgcta tcaggatcat
    32161 atgatcaaca ccctcgccga tgctcgtcgt tacagcgtcg aaaacaatct taaacatgtg
    32221 cagattatcg gcgatttcta tcacatgaac atcgaagaag ataacctggc gcaggcgctg
    32281 catgacaacc gcgacttgct cggtcatgtg catattgccg ataaccatcg ttaccagccg
    32341 ggcagcggca ccctcgattt ccacgcgctg tttgaacagc tgcgcgccga taactatcag
    32401 ggttatgtgg tgtatgaagg gcgtatccgg gcagaagatc ctgcccaggc gtaccgtgat
    32461 tcgttgacct ggttgcgtac ctgctaagag gtctttgtga aaagtgcaat gacaagctct
    32521 ccactgcggg tcgcgataat aggcgcaggc caggtggcgg ataaggttca tgcttcgtac
    32581 tactgcaccc gcaacgatct ggaactggtg gctgtctgtg acagccgcct ttcccaggcg
    32641 caggcgctgg cagaaaaata cgggaatgca tccgtgtggg acgatccgca ggccatgctg
    32701 ctggcggtga aacctgatgt ggttagcgtc tgctcaccta accgttttca ttacgaacat
    32761 accctgatgg cgctggaagc gggctgccat gtgatgtgcg aaaaaccgcc cgccatgacg
    32821 ccagaacagg cgcgggaaat gtgcgatacc gcgcgcaaac agggcaaggt gctggcctac
    32881 gactttcacc atcgttttgc gctcgatacg caacagctgc gtgaacaggt gaccaacggc
    32941 gttttgggag agatttacgt taccaccgcc cgcgccctgc gtcgctgcgg cgttcccggc
    33001 tggggtgtct ttaccaataa agaactgcag ggtggtggcc cgctgatcga catcggcatt
    33061 catatgctgg atgctgcgat gtatgtgctg ggctatcctg cggtgaaaag cgtgaatgcg
    33121 catagctttc aaaagatcgg cacgcaaaag agctgtggtc aatttggcga gtgggatccg
    33181 gcaacttaca gcgtcgaaga ttcgctgttt ggcaccattg aatttcataa cggcggcatt
    33241 ctgtggctgg aaacgtcatt tgcgctcaac atccgcgaac agtcgattat gaacgtcagc
    33301 ttttgtggtg ataaagccgg tgcgacgctg tttccagcac atatctacac cgataacaac
    33361 ggtgaattaa tgacgctgat gcaacgggaa atggcagacg acaaccgcca tttgggcagc
    33421 atggaagcct ttatcaatca cgtacagggc aagcccgtga tgatagccga cgccgagcag
    33481 gggtacatca tccagcaact ggtggcggcg ttatatcaat ccgcagaaac agggacgcgt
    33541 gtggaattat gaccaggcca gtaacgttat cagaacccca tttcagccag cataccctga
    33601 acaagtatgc atcgctgatg gcgcagggga acggctatct tgggcttcgc gccagccatg
    33661 aagaagatta cacgcgccag acgcgaggga tgtatctggc ggggctgtat catcgggcgg
    33721 gaaaaggtga aatcaacgaa ctggtgaacc tgcctgatat cttggggatg gagattgcca
    33781 taaatggtga ggttttctcg ttatcccacg aagcctggca gcgtgagctt gactttgcca
    33841 gtggcgaatt acgccgcaac gttgtctggc gtaccagcaa cggcgcaggt tacaccatcg
    33901 ccagccgtcg ctttgtttcg gcagaccaac tgccgctcat tgcgctggaa atcactatta
    33961 cgccactgga cgccgacgcg tcagtgctga tttcaacagg tatcgacgcc acgcaaacca
    34021 accacggtcg ccaacatctc gacgaaaccc aggtgcgggt gtttggtcag catctgatgc
    34081 aggggatcta caccacccag gatggacgca gtgatgtggc catcagctgt tgctgtaagg
    34141 tgagcggtga tgtgcagcaa tgctataccg ccaaagagcg ccgtttgctg caacatacca
    34201 gtgcgcagct tcatgcaggc gagacagtga cgttgcaaaa actggtgtgg atcgactggc
    34261 gggatgacag gcaagccgtt ttagacgagt ggggcagcgc gtcgcttcgc cagcttgaaa
    34321 tatgcgcgca gcagagttac gaccaacttc ttgcagcatc aacagaaaac tggcgtcaat
    34381 ggtggcagaa acgtcgtatc acggtaaatg gcggcgatgc gcacgatcag caagcgttag
    34441 attatgcgct ttatcatctg cgcatcatga cgccggctca cgacgagcgc agcagtattg
    34501 cggcaaaagg cttaaccggc gaaggctaca aaggccacgt tttctgggat acagaagtat
    34561 ttttgctgcc gttccatctg tttagcgatc cgacggttgc ccgaagttta ctgcgttatc
    34621 gctggcacaa cttgccaggc gcgcaggaga aagcacggcg cagcggctgg cagggcgcgc
    34681 tatttccgtg ggaaagcgcg cgcagcggcg aagaagagac gccagaattt gccgccatta
    34741 acattcgtac cgggctgcgg caaaaagtgg cctcggcgca ggcggaacat catctggtgg
    34801 ccgatatcgc ctgggcggtt attcaatact ggcagaccac gggggatgaa agtttcattg
    34861 ctcatgaagg catggcgcta cttctggaaa ctgcaaagtt ctggattagc cgcgcggtga
    34921 gggttaacga ccgtctggaa attcatgatg ttattgggcc agacgaatat accgaacatg
    34981 tcaataataa cgccttcacc agctatatgg cccgctacaa cgttcaacag gcgctgaata
    35041 ttgcccgcca gttcggctgt agcgacgatg cgtttatcca tcgcgccgaa atgttcctca
    35101 aagagctatg gatgccagaa acgcagcccg atggcgtttt gccgcaggat gattcgttta
    35161 tggctaagcc ggcgattaat ctggctaaat acaaagcggc ggcggggaag caaaccattc
    35221 tgctggatta ttcacgcgca gaagtgaacg agatgcagat cctcaaacaa gctgatgtgg
    35281 tgatgctcaa ttacatgctg ccggagcagt tctcagcggt atcgtgtctt gccaatctgc
    35341 aattttatga accgcgcact attcacgact cgtcattaag taaagcaatc cacggcattg
    35401 ttgccgcacg ctgtggcctg ctgacccaaa gttatcagtt ctggcgcgag gggactgaaa
    35461 tcgatcttgg tgctgatccg catagttgtg atgatggtat ccacgctgcc gcaactggcg
    35521 ctatctggct gggggcgatt cagggttttg ccggggtgag cgtgcgtgac ggtgaattac
    35581 atctcaatcc ggcgttaccg gagcagtggc aacagttgtc tttccctctg ttctggcagg
    35641 gctgcgaatt acaggtcacg ctcgacgcgc agcgtattgc gattcgaact tctgcgcccg
    35701 tttcactgcg tttgaacggg cagcttatat ccgtggctga agaatctgtt ttctgtttgg
    35761 gtgattttat tttgcccttc aatgggaccg ctaccacgca tcaggaggat gaatgaaact
    35821 gcaaggggta attttcgatc tggatggtgt aatcaccgat accgcgcatc tgcatttcca
    35881 ggcgtggcag cagattgccg ctgaaattgg catcagcatt gatgcgcagt ttaacgaatc
    35941 cctaaaaggg atcagccgcg atgagtctct gcggcgcatt ctgcaacacg ggggcaaaga
    36001 gggcgacttt aacccgcagg agcggacgca actggcgtat cgcaaaaatc tgctttacgt
    36061 ccactcactg cgcgagctga cggttaatgc tgtgctgccc ggtattcgca atttgctggc
    36121 agagctccgc gcacagcaga tcccggtagg gctggcttct gtctccctga atgcgccgac
    36181 gattttagcg gcgctggagc tgcgcgagtt tttcaccttc tgcgcggatg cttcccaact
    36241 taaaaactcg aaaccggatc cggaaatctt tctcgctgcc tgtgccgggc tgggcgtgcc
    36301 gccgcaggca tgtatcggca ttgaagatgc gcaggcgggc attgacgcca ttaacgccag
    36361 cggtatgcgc tcggtgggga tcggcgcagg cttaaccggg gcgcaattat tgttgccctc
    36421 aacggactcc ctgacctggc cgcggttatc ggccttctgg caaaacgtat agcaaaggaa
    36481 tcaacatggc tcagctttcg ttacaacata ttcaaaaaat ctacgataac caggtgcatg
    36541 tggtgaagga cttcaacctg gaaattgccg ataaagagtt catcgtattt gtcggcccgt
    36601 cgggctgcgg taaatcgacc accctgcgca tgattgccgg gcttgaggag atcagcggcg
    36661 gcgatctgtt gatcgacggc aaacgaatga atgacgttcc agccaaagca cgcaatatcg
    36721 caatggtgtt ccagaactac gcgttgtatc cgcatatgac ggtttacgac aacatggcgt
    36781 ttggcctgaa gatgcaaaaa atcgccaaag aggtgattga tgagcgggtg aactgggcgg
    36841 cgcaaattct cggcctgcgt gagtacctga agcgcaagcc gggcgcgctt tccggcgggc
    36901 aacgccagcg tgtggcgctc gggcgggcga ttgtgcgcga agcgggcgtg tttttaatgg
    36961 atgaaccgct ctctaacctt gatgccaagc tgcgcgtaca aatgcgcgca gagatcagca
    37021 agctgcatca gaaactgaac accaccatga tctacgtgac ccacgatcag accgaagcga
    37081 tgaccatggc gacgcggatt gtgattatga aagacgggat tgttcagcaa gtgggtgcgc
    37141 cgaaaaccgt ttataaccaa cccgcgaata tgtttgttgc cggatttatt ggatcaccag
    37201 cgatgaattt tattcgcggc acgatcgatg gcgataaatt cgttacggaa acgcttaaat
    37261 taaccattcc cgaagagaaa ttagcggttc tgaaaacaca ggaaagtttg cataagccca
    37321 tcgtgatggg aatgcgcccg gaagatattc atccggacgc gcaagaggaa aataatattt
    37381 ccgccaaaat tagcgtggta gaattaaccg gtgcggaatt tatgctctac accacggttg
    37441 gggggcacga gttagtggtc cgtgctggtg cgttaaatga ttatcatgca ggagaaaata
    37501 tcactattca ttttgatatg actaaatgtc atttctttga tgcagaaacg gaaatagcaa
    37561 ttcgctaaat acagggggaa ggcattcccc caggataata caaggaacaa taatgaaaaa
    37621 gttattaccc tgtaccgcac tggtgatgtg tgcgggaatg gcctgcgcac aggccgagga
    37681 aaagaacgac tggcacttta atatcggcgc gatgtacgaa atagaaaacg tcgagggtta
    37741 tggcgaagat atggatgggc tggcggagcc ttctgtctat tttaatgccg ccaacgggcc
    37801 gtggagaatt tctctggcct attatcagga agggccggta gattatagcg cgggtaaacg
    37861 tggaacgtgg tttgatcgcc cggagctgga ggtacattat cagttcctcg aaagcgatga
    37921 tttcagtttc ggcctgaccg gcggtttccg taattatggt tatcactacg ttgatgaacc
    37981 gggtaaagac acggcgaata tgcagcgctg gaaaatcgcg ccagactggg atgtgaaact
    38041 gactgacgat ttacgtttca acggttggtt gtcgatgtat aaatttgcca acgatttgaa
    38101 cacgaccggt tacgctgata cccgtgtcga aacggaaaca ggtctgcaat ataccttcaa
    38161 cgaaacggtt gccttgcgag tgaactatta tctcgagcgc ggcttcaata tggacgacag
    38221 ccgtaataac ggtgagtttt ccacgcaaga aattcgcgcc tatttgccgc tgacgctcgg
    38281 caaccactcg gtgacgccgt atacgcgcat tgggctggat cgctggagta actgggactg
    38341 gcaggatgat attgaacgtg aaggccatga ttttaaccgt gttggtttat tttacggtta
    38401 tgatttccag aatggtcttt ccatttcgct ggaatacgcg tttgagtggc aggatcacga
    38461 cgaaggcgac agcgataaat tccattatgc aggtgtcggc gtaaattact cgttctgata
    38521 atgggctaaa ttgccggatg cggcgcgagt actttatccg atctataaat gtaggccgga
    38581 taagatgcgc cagcatcgca tccggcattc aggcaaggtc gctggtattt atttcagcgt
    38641 catatgcgtg gcaacggtaa tattttgtgg tgacggtttt ccggcaatta agcggaataa
    38701 taactcacag ctttgtcgac ctaactcctg cgtcggaaca tcgatgccgc ccggtgcagg
    38761 cgttaaaata aatgacagcg tttcattgct ataacccacc accgctaact gctgcggaat
    38821 agcaatgttt ttctctgccg ccgcacgata aatgctcatt aatttcaggc tgtcagtggc
    38881 aaacactgcc tcaggcaacg gtgactgact taataattgc cgtgctgctt gcagtgctgt
    38941 ttcatgggta taaccgccat caacaatcca ttcatcacgc actgcaatat tatgatcttc
    39001 cagactctgc ttataaccat ttacccgatc cactgaaaca tggacatcaa gcggtgcatg
    39061 cagacaggca atattttgat gtccactttc aattaacgca tcggtcaacg caatgctgtc
    39121 accaaaatta tcggtatcga cagaataaac atgggcatat tgaccttcaa ctttgccaat
    39181 caccaccaca ggaatatcgt atttatcaag ttgggcaaaa aatgactcat ccgctggcga
    39241 acttagcata ataatgcctt taatcatttt ctgcttaatt ttgctttcac atttttgtaa
    39301 gtcttctgcc ggggtgtgcg acgtctgcaa tatcacgtcg aaaccttctt cttcagcttt
    39361 ggcggtgatg gcatgcaaaa cttcagagaa aaacggatta cccgccgtag ttttggtcga
    39421 acgggtagaa atcaccataa tggcatcaaa acccgaagag gtcagcgcgc gggccagctt
    39481 gtttggttga tactgtaatt cttcaatggc ccgtaacact ttttcgcgcg cttccgggga
    39541 gatattggtt tgcttattca gcacgcgtga tacggtggat tttgatacgc ctgcaaccct
    39601 ggcaatatca taaatagtag gggacatagg tcaggaactc cgtccgaacg tgaatgctgc
    39661 acatcttatg gagttcgccc gtcgatgaca acacgggtag ttgataatca atggcctggc
    39721 ccccacattc atatccttat gaatgatttt tttctggtct tcagagcagc acaggacagc
    39781 aatgaagcga cttaaaaatg aacttaatgc gctggtgaat cggggtgtcg acagacatct
    39841 gcgcctcgcc gtaaccggac ttagccgcag cggcaaaacg gcgtttatca ctgcgatggt
    39901 caatcagttg ctcaatattc atgccggagc acgtttgccg ctattaagtg cggtgcgtga
    39961 agagcgtctg ctgggcgtaa aacgcattcc tcagcgtgac tttggcattc cgcgttttac
    40021 ctacgacgaa gggttggcgc agctgtatgg cgatcctccc gcctggccga cgccaacgcg
    40081 cggcgtcagc gaaattcgcc tggcactacg ctataaatcg aacgattcgc tgctgcgcca
    40141 ctttaaggat acctccacgc tgtatctgga gattgtggat taccctggcg aatggttgct
    40201 cgacctgccg atgctggcgc aggactattt aagctggtcg cgccagatga cgggcttact
    40261 caatggtcag cgcggcgaat ggtcggcgaa atggcgaatg atgtgcgaag ggctggaccc
    40321 gctagcgcct gccgacgaaa accggctggc agacattgcc gccgcgtgga ccgattatct
    40381 ccaccactgt aaacagcagg ggctgcactt tattcagcct gggcgctttg tcttgccggg
    40441 ggatatggca ggtgcgcccg cgctgcaatt cttcccgtgg ccggatgtcg atgcctgggg
    40501 cgagtccaaa ctggcgcagg ccgataagca caccaatgcc ggaatgctgc gcgagcggtt
    40561 taattattac tgcgagaagg tggtgaaggg gttctataag aatcattttc tgcgctttga
    40621 ccgccagatt gtgctggtgg attgcctgca acctctcaac agtgggccac aggcatttaa
    40681 tgatatgcgt ctggcgctga cgcagctgat gcaaagtttc cactatgggc agcgtaccct
    40741 gttcaggcgt ttgttttcgc cggttatcga taagctattg tttgctgcca ctaaagcgga
    40801 ccatgtgacc atcgatcagc acgccaatat ggtttcattg ctgcaacaac taattcagga
    40861 tgcctggcaa aatgcggcgt tcgaagggat cagcatggat tgcctggggc tggcgtcagt
    40921 tcaggcgacc accagtggca ttattgatgt taacggtgag aaaatcccgg cgctgcgcgg
    40981 taatcgactt agcgatggcg caccgctcac tgtttatcct ggcgaagttc ccgcacgttt
    41041 gcctggtcag gcgttctggg ataagcaagg gttccagttt gaagcgtttc gcccgcaggt
    41101 gatggatgtc gacaaaccgc tgccgcatat tcgtcttgat gccgcgctgg aatttttaat
    41161 aggagataaa ttgcgatgac cgaaccgtta aaaccacgta ttgatttcga cggtccgctg
    41221 gaggtcgaac agaatccaaa attcagggcg cagcagacct ttgacgaaaa tcaggcgcaa
    41281 aattttgccc cggccacgct cgacgaagcg caggaagaag aggggcaagt cgaagcggta
    41341 atggacgcag cgttacgtcc gaaacgcagc ctgtggcgca aaatggtgat gggcgggctg
    41401 gctctgtttg gcgcaagcgt tgtcgggcag ggtgtacagt ggacaatgaa tgcctggcaa
    41461 actcaggact gggtggcgct gggtggatgt gctgctgggg cattgattat cggcgctggc
    41521 gtaggttctg tggtaacaga gtggcggcgc ttatggcgct tgcgacagcg cgcccatgaa
    41581 cgcgacgaag cgcgcgattt gttgcacagc cacggcacgg gcaaaggccg cgcattttgc
    41641 gaaaaactgg cgcagcaggc gggtattgat cagtctcatc cagcgctgca acgctggtat
    41701 gcctcaatcc atgaaacgca gaacgatcgt gaagtggtca gcctgtatgc tcatctggtc
    41761 cagccggttt tagatgccca ggcgcggcgc gaaatcagcc gctcagcagc tgaatcaacg
    41821 ttgatgattg cggtcagccc gctggcgctg gtggatatgg catttatcgc ctggcgcaat
    41881 ctgcgtttga ttaatcgcat cgccacgctg tatggcattg aactgggata ttacagccgt
    41941 ttgcgcctgt tcaagctggt attgctgaat atcgctttcg ccggagccag cgaattggtg
    42001 cgcgaagtgg gaatggactg gatgtcgcaa gatctcgctg ctcgtttgtc tacccgcgca
    42061 gctcagggga ttggtgcagg acttctgacg gcacgactgg ggattaaagc tatggagctt
    42121 tgccgcccgc tgccgtggat tgacgatgac aaacctcgcc tcggggattt tcgtcgtcag
    42181 cttatcggtc aggtgaaaga aacgctgcaa aaaggcaaaa cgcccagcga aaaataatgc
    42241 aatatcgggt gctgaccgga tatctttacg ccgaagtgcc cgtttttccg tctttgtgtc
    42301 aatgattgtt gacagaaacc ttcctgctat tcaaatagtg tcatatcatc atattaattg
    42361 ttcttttttc aggtgaaggt tcccatgcgt ctggaagtct tttgtgaaga ccgactcggt
    42421 ctgacccgcg aattactcga tctactcgtg ctaagaggca ttgatttacg cggtattgag
    42481 attgatccca ttgggcgaat ctacctcaat tttgctgaac tggagtttga gagtttcagc
    42541 agtctgatgg ccgaaatacg ccgtattgca ggtgttaccg atgtgcgtac tgtcccgtgg
    42601 atgccttccg aacgtgagca tctggcgttg agcgcgttac tggaggcgtt gcctgaacct
    42661 gtgctctctg tcgatatgaa aagcaaagtg gatatggcga acccggcgag ctgtcagctt
    42721 tttgggcaaa aattggatcg actgcgcaac cataccgccg cacaattgat taacggcttt
    42781 aattttttac gttggctgga aagcgaaccg caagattccc ataacgagca tgttgttatt
    42841 aatggacaga acttcctgat ggagattacg cctgtttatc ttcaggatga aaatgatcaa
    42901 cacgtcctga caggtgcggt ggtgatgttg cgatcaacga ttcgtatggg ccgtcagttg
    42961 caaaatgtcg ccgcccagga cgtcagcgcc ttcagtcaaa ttgtcgctgt cagcccgaaa
    43021 atgaagcatg ttgtcgaaca ggcgcagaaa ctggcgatgc taagcgcgcc gctgctgatt
    43081 acgggtgaca caggtacagg taaagatctt tttgcctacg cctgccatca ggcaagccca
    43141 agagcgagca aaccttacct ggcgctgaac tgtgcgtcta taccggaaga tgcggtcgag
    43201 agtgagctgt ttggtcatgc tccggaaggg aagaaaggat tctttgagca ggcgaacggt
    43261 ggttcggtgc tgttggatga aataggtgag atgtcaccac ggatgcaggc gaaattactg
    43321 cgtttcctta atgatggcac tttccgtcgg gttggcgaag accatgaggt gcatgtcgat
    43381 gtgcgggtga tttgcgctac gcagaagaat ctggtcgaac tggtgcaaaa aggcatgttc
    43441 cgtgaagatc tctattatcg tctgaacgtg ttgacgctca acctgccgcc gctacgtgac
    43501 tgtccgcagg acatcatgcc gttaactgag ctgttcgtcg cccgctttgc cgacgagcag
    43561 ggcgtgccgc gtccgaaact ggccgctgac ctgaatactg tacttacgcg ttatgcgtgg
    43621 ccgggaaatg tgcggcagtt aaagaacgct atctatcgcg cactgacaca actggacggt
    43681 tatgagctgc gtccacagga tattttgttg ccggattatg acgccgcaac ggtagccgtg
    43741 ggcgaagatg cgatggaagg ttcgctggac gaaatcacca gccgttttga acgctcggta
    43801 ttaacccagc tttatcgcaa ttatcccagc acgcgcaaac tggcaaaacg tctcggcgtt
    43861 tcacataccg cgattgccaa taagttgcgg gaatatggct taagccagaa gaagaacgaa
    43921 gagtaagcgc gaatatgcct gatggtgcaa caccatcagg catattaact tatgctttca
    43981 gtacagccag agctgcttcg taatccggct cggtggtgat ttcatccacc agctggctga
    44041 aaatcacatt gtcattttca tcaataacca caacggcacg cgctgccaga cctttcagtg
    44101 ggccatcagc aattgccaca ccgtaggctt gcagaaattc agcgttacgg aaagtggaga
    44161 gggtgataac gttgttcaga ccttctgcgc cgcagaaacg agactgggcg aacggcagat
    44221 cggcagagat acacagcaca acggtgttgt cgatctcggt tgccagttgg ttaaacttac
    44281 gtactgatgc ggcgcaaaca ccggtatcaa tactcgggaa aatgttcagc actttgcgtt
    44341 tacccgcaaa ctgaccgagg gtgacgtcag acagatcttt tgccacgaga gtaaaagtct
    44401 gcgctttgct acccgcctgc gggatggaat tggcgactgt aaccgggttg ccctggaaat
    44461 gaacggtttg tgacatgatt atctttcctg tttacatata gttaacgtca cacctagttt
    44521 atgctaactg tcaataacac agcaaacgct atttgcgctt aatccgcaga ccaccgcgac
    44581 accaaggagt aaagatgaga accgttaagg tatttgagga agcctggccc ttacataccc
    44641 cgtttgtgat tgcccgggga agtcgcagtg aagcgcgcgt ggtggtggtt gaactggaag
    44701 aagagggtat taaaggcacc ggcgaatgca cgccgtatcc gcgttatgga gaaagtgatg
    44761 cctcggtaat ggcgcaaatt atgagcgtcg tgccgcaatt agagaaaggg ctgacacggg
    44821 aggagttgca aaaaattctc cctgccggcg cagcacgtaa tgcgctggat tgtgcattgt
    44881 gggatctggc cgcgcgaaaa cagcagcaat cgctggctga tttgatcggc ataacgcttc
    44941 ccgagacagt tatcactgca cagacggtag tcatcggtac gcctgatcag atggccaata
    45001 gtgcatcaac actctggcag gccggcgcga aattactgaa agtgaagctg gataaccatc
    45061 ttatcagtga gcggatggtg gcaattcgca cagctgtgcc cgataccacg ctgatcgttg
    45121 atgcaaatga atcctggcat gcagaagggt tggcggcacg ttgccagcta ttggcggatt
    45181 taggcgttgc gatgcttgaa caaccgcttc ctgcgcagga cgatgcggcg ctggagaatt
    45241 ttattcatcc gttgccgatt tgtgctgatg aaagttgtca tactcgtagc aatttgaagg
    45301 cgctgaaagg gcgctatgag atggttaata ttaagctcga taaaaccggg ggtctgacgg
    45361 aagcgctggc gctggcgact gaagcgcgtg cacaaggttt cagtctgatg ctgggctgca
    45421 tgttgtgtac ctctcgggcc attagcgccg ctttaccgct ggtgccgcag gtcagtttcg
    45481 ccgatcttga cggaccgacc tggctggcgg tagatgtgga accggcgctt cagttcacga
    45541 cgggcgaatt gcatctttag gatgccagcg cagcaagttt gccatcgcaa agagatattt
    45601 ttcgctggct tcgtcggagg agatgggcgg aaactctgcg gtgatgcaat gcaagttcag
    45661 atctgcacac caactgccga aagagccagg cgtttcataa ccgacgctgg ttaccagagg
    45721 caattcaaac gcctgagcca gccattcgcc taattcgctg tgtctgggat cttcaataca
    45781 ggccagtgga tcgtggaatg agaccaccca ggcaggttga atgcggtgta taagctggca
    45841 cagcgcctgg gtttcaggtt cggagccagg tttgtcacct gtcagtaata cgacatcacg
    45901 ttcttcagcg gcgctgttcc agcgataaac cgtttcacct tccttccagt tcgccgccgg
    45961 aaagtttctg tttaaatcca caccattagc attggcccgt aaccccagct ggcagccgtc
    46021 aggattcaca cacagcacca catgatggcg gcgcaaagaa ggtgtcaaag tccgcagagc
    46081 acaggagagc gtgacgaccg aagagttttc atcaccgtga gtgccagcca gaatcaaacc
    46141 actttcgcga ctggctgcag gggccggaaa ccagattaac ggcgcgccca ataatgaacg
    46201 tccgtaatgt tctgttccgg gcggaaatgc gccgcgttcg gcgcgtgggc gggttacggt
    46261 catgatcgtc tctggttcag gaagtattac aggcagtgtt gtgcaaattt tgcggattat
    46321 caaatccttt tcttcaacgc tatgtcatcg atcttgtatc acttttttgc cggaagttgt
    46381 ttgtattttc attgatgtgc agatattaga attgtcgtaa tgttatatac atggcgtatt
    46441 ttattcttac tggttctaca catgttaatc gcatctgatt gatcagttca ccttaataag
    46501 tttaataaat cgtactgcac atagtacagt acgatggtgg ttatataatt gatttacatg
    46561 aagaatggct tgtcgccatc agtcccgggt tgattgatgc ctatattctc accaatgtgt
    46621 ttctcaggtt tatcgatgat atctgtaatt aatgctgcga cactcttagg tgatacgatc
    46681 gtacctttga atggttcatt gcgtgaggta agctcataat caataatatc ttcatccgtc
    46741 agccaggcag ggcgaagaat tgtatattcc agtccagaag cctcaattgc atccgcagcc
    46801 ctgcggaatg gttttagggg ctcaccaata actgcattat tccattcgcc aaattttcct
    46861 ggaacttcat cataaatgcc gagggaaaga acaaagatca gacgtttaac atcacaggct
    46921 ttcatcgcag cgatcactga atttgcctga atatctaaat cttcccccgt aaggttggca
    46981 tacacaatat cctgtccctg catggcctgc ttcagtgctg catgattcag tacatccccc
    47041 ataataatct ggctattagt tggatagggt ttgtgtattt ttgctggctg acgagcaaat
    47101 aaagtttgtt taatggtttg tttatctgcc agttgattaa tcacatggcg ggctatctga
    47161 ccgccagcac caagaatgag tacatttttc ataatatttc tcatgaaact tatcacagat
    47221 gggtgttgaa aaaaggaata actttttcca gcgcctcctg aactgcttcc ggtttgtcat
    47281 acagttcata atgtgagaaa tcgggtaatg aaactaactg acgatcctgc gaaaccatcg
    47341 cccggccata gacttccagt ccatcacgat aagcaccgaa ggcaccgact ttttcaccaa
    47401 ctactaccat caccggctga gtcagtaaca cttcagtaaa tgcgaaggca tcccaggcca
    47461 gcgttttctg tgcatgtgag aacaacattt ttgtcgcacc tcccggttgt tgcccgcgtg
    47521 gtgttttata gtagtcagtt gcttcataaa catcgcgttc ggtcagtcca tgggcttttg
    47581 ctgcatccag gctggcggga agtaattcgt tgatctgtaa ttctccacca cgagcctctt
    47641 tggttcgttg cgacgccatt gcattcaatg cgccaatcgg atcgtaattg ctgaaacctt
    47701 cccgaaaaag acgcccgata ttaacacccg taatactcac tacagcttta attcgttttt
    47761 ctgtcagcgc ggcattaatg gcatagccac ccccccccac acacacccag cacgccaatg
    47821 cgttcagcat caacatatgg cagagttact gcataatcga taacacggct aatatcctct
    47881 acgcgctgtg tcggatcttc tatccaacga ggctccccac cagattctcc ctggaaactg
    47941 gcgtcataag cgagaacgac atagcctttt tcagccaatg ccttgccata gatattgcca
    48001 gacgtctgct ccttacaact gccaaaagga tggacgctga taatcgccgg atactgtttt
    48061 gactcatcaa aatttggtgg aaactgaata ttggcagcaa tatgccaggc catatcacta
    48121 ttgcggattt gaatatgttt cattacagag tttcctcaaa gaacggagct aatactgaaa
    48181 ttgcttcagc cacataggct ttgccgtcat aaagatccat atggtttgcg ccttcaacaa
    48241 tgtgatagcg tttatcctgg cttgatgccc gatcgtacag gtcgtcactc atccatttgc
    48301 tccctgcctg gctgcccgcc acaatctgcg tcggctgagt caggtacact tccgccatat
    48361 ggtaagcatc ataggtaata atctggttaa ggctgcgcaa agtagcgtaa cccggtgctg
    48421 ttggatactg cgcgcgaggg gtgtggtaat actcccaggc ctgacgcagt tcttcgttcg
    48481 gcgcatcgga ctccttcatt ggtgccagtg gcataatggc gtattctccg ctgctaatat
    48541 cgctggttct ggcgtttgaa cccgcttcaa cgtatggcag ggcatcaata gatttcacat
    48601 tgttttccca accattacgg aacatcgaac caatattgac cgcactaacg gtaccgatgg
    48661 ccttgatgcg gcgatcctga attgcagcat tggctgtata tcctgcaccg gcacaaattc
    48721 ccatcgcacc aattcgggta ttgtcgacat aagaaagcgt tgtcaggtaa tcaatcacgg
    48781 cactgacgtc ttcagtacga atgtatgggt tttctaactg acgcggctcg ccgccacttt
    48841 caccctgata agatgcgtca taagcaatag tgacaaatcc cttttccgcc agtttttcgg
    48901 cataggtccc ggccgtttgt tctttaacgc ccccacctgg gtgagatacc acaattgccg
    48961 gatattgacg ggtttcatca aattttggag gaaaatagat cactgcagac aaagagatgg
    49021 ttggattatt gctgttagtg aagctgactt tattattcat cattccgttc ctcatggagt
    49081 tgttggttcg ttttaacggt tggtgatatc actatagata ttgatcatta agttgattag
    49141 accctaaatc atgattagac ctatcattta aatgattaat agattgtatg gagatactga
    49201 atgaagcgtg aagagatcgc tgatctgatg gcgtttgtcg tcgttgcaga ggagcgtagc
    49261 ttcactcgcg cagcagcccg cctgagcatg gcgcagtcag ctttaagcca gatagtgcgc
    49321 cgtatagaag aacgattggg attgcggctt ctgacgcgaa ccacgcgcag tgttgtaccc
    49381 actgaagcgg gcgagcatct tttgtctgtt cttggcccta tgctgcatga catagattct
    49441 gccctggcat ccctgagcga tctgcagaac cgcccatccg ggacaatacg tattactact
    49501 gtagaacatg cagcaaaaac gatattgtta ccagccatgc gcacattcct gaaatcacac
    49561 cctgaaattg atattcagct tatcattgat tatggtttga ctgatgtcgt ttctgaacgt
    49621 tttgatgcag gcgtccgtct gggtggggag atggataaag atatgatcgc cattcgaatc
    49681 gggccagata taccaatggc tattgttggc tcaccggatt atttttctcg ccgaagtgtt
    49741 ccaacgtcag tgtcacaatt aatagatcat caggcaatta atttgtatct tcccacatcg
    49801 ggtacagcaa atcgctggag attaatacgc ggtggacgtg aagttcgtgt tcgcatggaa
    49861 ggtcagcttt tactgaatac gatagacctg atcattgatg ctgcaattga tgggcatgga
    49921 ttggcgtatc taccttatga tcaggttgag cgggcgatta aagaaaaaaa actgatacgt
    49981 gttttggata aattcacacc agatttaccc ggttatcacc tgtactatcc acaccgtcga
    50041 catgctggct cggcattctc attatttata gataggctga agtataaagg tgctgtttag
    50101 cactacttgc tgatacatta atttaattct tctcttaacg tattctcagt tcctttcaac
    50161 gttttggtta ttttttattc ttcgtacaat ggcgacagat gctgattatg ataccgaaaa
    50221 ctggtttgaa cgtgtgaagc ccgaacgtag tgttcggtgt tctatgtgct ttgccgcatt
    50281 ttggcgtcta ttatttacac taaatctgat ttaatatatt gatacataaa acattttatg
    50341 cttcctgtgt cactttctga tggaagttgt ttgcatttct ttaaggcgaa acaaataatt
    50401 acgcatcaat tttaatgtcg gttagaggga aacttatgaa gcactctgtt tcagtcacgt
    50461 gttgtgcgct gttggtcagc agcatttctc tttcgtatgc tgcagaagtt ccgagcggca
    50521 cagtactggc agagaagcag gagctggtgc gccacattaa agatgagcct gcgtcgctgg
    50581 atcccgctaa agccgtgggc ctgccagaga ttcaggtcat tcgcgatctg tttgaaggac
    50641 tggtgaatca gaacgagaaa ggggagattg tccccggcgt tgcgactcag tggaaaagta
    50701 atgacaaccg tatctggact tttaccctgc gcgataacgc aaaatgggcg gatggcacac
    50761 cggtaacggc gcaagatttt gtctgcagct ggcagcgtct ggtggacccg aaaacgctat
    50821 cgccatttgc atggtttgcc gcgctggcgg gaatcaacaa cgcacaggcg attattgatg
    50881 gtaaagctac acctgaccag cttggcgtca ccgcagttga tgtccatact ttgaaaattc
    50941 agcttgataa accgttgccg tggtttgtga atttaaccgc taactttgcc ttcttcccgg
    51001 tgcaaaaagc caacgtagaa agcggtaaag agtggacgaa acccggaaat ctgatcggca
    51061 atggcgctta tgttcttaaa gagcgcgtag tcaatgaaaa actggtcgtg gtaccgaata
    51121 cccattattg ggataacgcc aaaacggtac tgcaaaaagt gaccttcctg ccaattaatc
    51181 aggaatccgc agccactaag cgttacctcg cgggggatat tgatatcacc gaatccttcc
    51241 cgaaaaatat gtatcagaag ctgttgaagg atattccggg gcaggtttat acgccgccgc
    51301 agctcgggac ctattattat gcgtttaaca cgcaaaaagg gccgacggca gatcagcgcg
    51361 ttcgtctggc attaagtatg acgatagatc gccgcctgat gaccgaaaaa gtattaggga
    51421 cgggcgaaaa gccagcgtgg cattttacac cagatgttac cgccggattt acgccggaac
    51481 cttcgccgtt tgaacaaatg agtcaggaag aactgaatgc gcaggcaaaa actttgttga
    51541 gcgcagctgg ttatggtccg caaaaaccgc tgaagctgac gcttttgtat aacacttcag
    51601 aaaaccatca aaaaattgcg attgctgtag catcgatgtg gaaaaagaac cttggcgtag
    51661 atgttaaatt gcaaaatcag gaatggaaaa cctatatcga tagccgtaac accggcaatt
    51721 ttgatgttat ccgcgcctca tgggtggggg attataatga accctccact ttcctgacat
    51781 tattaacgtc aacgcattca ggaaatattt cacgctttaa caatccggca tacgacaaag
    51841 ttctggccca ggcatcgacg gaaaataccg ttaaagcgcg taatgccgat tacaacgcgg
    51901 cagaaaaaat cctcatggag caagcaccga ttgcaccaat ttatcaatat accaatggac
    51961 gattaatcaa accgtggctg aaaggttatc ccattaataa ccctgaggat gtggcgtaca
    52021 gtcggactat gtatattgtg aagcattgat gtgatgggaa ctggcgttac ccttgtggat
    52081 aacgccagtg atgtctgatt tagcgaccct gttccggcgg tgtaatgtta tccatataca
    52141 gcgtctggct ggggaaggca aagtccgcgc cgtgtgactg tacaatatcg ataatcttca
    52201 aataaacgtc ttgctgtgca gcaagccact cagcccatac cgtggttttg gtaaagcaat
    52261 aaaccataat attcaatgac gagtcagcaa actggttgaa ataaaccagt aaggtttgtc
    52321 gctggtcgat ggccgggtga tttttcagca tctcacgtac ggcttcgacg ataacgccca
    52381 cttttgccgc atcctcataa cgtaaaccaa tggtcgtggt aatgcggcgg ttggtcattc
    52441 gtcctgggtt ttctacgctg atcgatgaaa acagcgagtt cggtacgtac aatggacgat
    52501 tatcaaaggt cgtaattttg gtaattcgcc agccaatttc cgctactgta ccttcgatat
    52561 ttctgtccgg tgaacggatc cagtcgccaa tactgaaagg acggtcgaaa tagagcatga
    52621 tcccggaaaa gaaattactc agaatatctt tgccagccat accgacagcc agaccaccaa
    52681 taccaccaaa ggtcagcaag ccagaaaggc tcatgccgaa atgttcgcca taaagcagaa
    52741 caagcaccac aataatggtg attttgataa tgcgcgacat aatccgcgca ctggtgatat
    52801 cgcgaccctt tttaatctgc tgtttttcaa actgattaat cagcaaaaat agcttaatcg
    52861 tcagaataac cgcaatcagg gacgtacaga taaaatcgat aacgcctgga gtgataaatt
    52921 tgagtttata gttttctata acataattaa taatgctacc aacagcactg ataattatgg
    52981 tgtagattaa aaattgcacc gcatggaata aaaagccttt tcttttacga tttccgcggc
    53041 gaaaccaaaa gctcatcaga atcaatgctg cgcagctacc gaaaataatg accagattaa
    53101 gcgcattatt tgtaaacagt tcagcgatca ttgttttatc aggctcctcc agataattgt
    53161 cgtcatgccg gaaacccctg gcggggctat tttaccgcga caattcattc agatcatcaa
    53221 tagtcaggga aggaagtagc aacattagct aactaatcaa tgcgttgatt gtaaatccag
    53281 ctaagaggtg aggttttcag agcagacaat ggtgaaatgt catggtattg ttacgtttag
    53341 gtaacaagaa atttgtctgc acaaggatta catcatgatt atggcgaaac tgaagtcagc
    53401 gaaagggaag aaatttctct ttggtttgtt ggcggttttc attattgcgg cgtcggttgt
    53461 gactcgcgcg actatcggcg gcgttataga acagtacaat attccgctgt cggagtggac
    53521 gacatcaatg tatgtgattc agtcatcgat gatttttgtt tatagcctgg tctttactgt
    53581 gttgctggca atcccgttgg gaatttattt ccttggcggc gaagagcagt aagtaaaaaa
    53641 taggcccgat aactcgggcc ttgtcagtta ttgaggagtc gttaatcgtc ttcttcgtca
    53701 tccagttcaa cgggtgtctg atactggtca ggtttaatga ccaacagatc gcagcgaaga
    53761 tgatcaatca cctgttccgc cgtgttgccg aggaatgctg ctgaaatacc ggtgcgtcct
    53821 accgtgccca gaaccacaat ccccgcctgt aagtgctccg ccaaatcagg aatcacctct
    53881 tctggcagac ctttttctac gtgcgtcatg ttttcattaa tgccgaattt ctgccgcagg
    53941 gctttcattg ccagcaaatg ttgcccacga atggcatcgt tataaacgct cggatcaaat
    54001 tccggcagtt caatcgcgat attaattggc gttaccggat aagcgccaac cagatgaact
    54061 tcggtatggt tgacttgttc tgccagttcg atcgtctctt tgaccagttt ttcattgagc
    54121 gcattatgat acggctcttc actggcgaga ttcaccgcca ccagcgcctt acctccttcc
    54181 ggccacggct ggtctttcac catccacacc gggcttgggc atttgcgtaa caggtgccag
    54241 tccgttggcg taaaaatcac cgcttccaga cggtcatgtt ggtgcgccat ttttagcacc
    54301 aaatcgtgcc cgccgctgat cacttcctga atgatggctt cgaaaggacg gttatgccag
    54361 accactttaa tttcaatggg aacgccagca ttgagataat attttgcctg ctcgtggatc
    54421 caggctgtac gctggctgat gacgccctga cgcatagcgg tacgttcgtc cggggagagc
    54481 agggtggtca tttcgtatga gaagtcatag atcggcaaaa aggctttaat tttgccacca
    54541 atccgttgat gtaaataaac agctcgccgc aatgctggtt ggtcgtcctg gttaggatcg
    54601 ataacaacga gcatgttctg atacatagcc atacagggtc tccttacaac aactgtcaac
    54661 gcagtttgta attaaaagat taacccatat ctggtgaatg aaacagtgat gaaccttctg
    54721 ccagatcaat aaatcagaaa aatttaatga tatgacagaa ggatagtgag ttatgcggaa
    54781 aaatcaggca acgttacgcg tatgaccagc aagctgggcc agcgtatcgt tattttcgat
    54841 ggtgatgtat ttacctttga ctgccagcat accgcttttc tggaagcgac ccagcagacg
    54901 gctgatggtt tctaccgtca ggcccagata gttaccgata tcgccacgag tcatcgtcag
    54961 gcggaattca cgaggggaga agccgcgttg ggcaaaacga cgggacaggt tgtagatgaa
    55021 tgcagccaga cgttcctcgg catttttctt tgacaacagc aggatcatgt cctgatcgcc
    55081 tttgatttca ccgctcatca gacgcatcat ctgctgacgc agattcggca ttttaccgga
    55141 caaatcgtcc agcgtttcga acgggatttc acataccatc gaggtttcca gcgcctgcgc
    55201 gaagctcggg tgatggccgc tgccgatggc gtcaaatccc accaggtcgc ctgctaaatg
    55261 gaaaccagtg atttgctcgt cgccttgctc agtgatggta taacttttaa tcgtaccgga
    55321 gcggatggca taaagcgatt taagttcatc accagcctta aacagcgtct ggcctttctg
    55381 aataggcttc ttccgctcaa tgatattatc aagctgatca agctcatgtt cgttgagtgt
    55441 gaacgggatg caaagctggc tgatgctgca atcctggcaa tggatagcac aaccgccaga
    55501 ctgaatgcgc cgtataattc gcttttccgg gatcataggt ctgctcaagc cgtaattgat
    55561 atttgtcaat tttaacatct ttttagggag caagtaagtc taagcaaacc ttaacagcag
    55621 agaattccga tattagatgt aaatatatgt ctatctattt gaaaaccctt aagttgttaa
    55681 gggtaacttt acataaaagt gtgaacaagc tggcacaaat tgtttaatgt ttacagcaaa
    55741 agataacctt cgtggcgcaa taaccactct tttcgctgaa ctccgcctgc atatccggtc
    55801 atggtgccgt ttcggccaat aacccgatgg caaggtacga cgatgctgat gggattcgat
    55861 ccgtttgccg caccaacggc acgcgccgcg ccaggacggc ccaattgctc agccagttcg
    55921 ccgtaatgca ttacctgccc gcaggggata gtgcgtagtg ttttccagac ttcgcgctga
    55981 aatggcgtcc cccccgtagc agtgggaagc gtatcaataa tgctaagatt accggcaaaa
    56041 tattcacgaa gcttgtcgct taaaccgcct ggattggtgg cagaaatgcg ctcatagcct
    56101 tctttgcgat aatggatgtc cagcagctgc accatgcgtt cgctgtactc ttcccattca
    56161 accgcccgca ggcgaaattg ctcatcgcaa atcacccaca gtggacccag tggtgtggca
    56221 attttttctt caagtaatct cagcatccgt tctctcttaa gacaaacgtg ggtaaatacc
    56281 cggacctatc ggcaggccca caagatacca cgccagcaac atcagcagcc ataccaccaa
    56341 aaagataagc ggatagggca agactaacga atagtaagta cccagtttcg cgtctggttt
    56401 gtagcgttgc aggaatccaa gaaacagtgg aacaaaagga gatactggcg ctaaaggcaa
    56461 tacggatgag tcggcaatac gaaagaggat ttgcgcaaat gccgggtgaa agccaagtag
    56521 cataaacatt ggtacgaaaa tgggggccag aatcgaccag attgcggaac cgctggcgat
    56581 aaacatgcat aagaaagagg aaagcaacgc cagaccgaca aacgccggga tgccgctaag
    56641 ccctgaactt tccaggatat cggtcagccc cacggcgatg aatttcccca tgttgctcca
    56701 gttaaacatg gcgacaaact gggcgagggg aaaaaccatc acgataaatc ccgccatctc
    56761 tttcatcggt tcaatcatta aatgcggtaa atccgcctga cgtcgaattg tgcgggtagc
    56821 gatgccataa gccagcgaga caacaaagaa aaaaagaatg atcagtggca cgataccttt
    56881 aataaagggt gatggcatca cggtgtgatt aatcggatcg cgcaatatcc cgttttccgg
    56941 gatcaccatc agcgcaatcg cagcaataaa aagtagcgat acgacacctg ctatgcgtaa
    57001 accaaaacgc tgactttcgg tcaatgtctg cagtttctca tcgctgtttc cctgccattg
    57061 acctaaccgt ggctcgatga ttttgtcggt tatcaggccg ccaacaatcg tcagtacgac
    57121 tacggagctg gccataaaat accagttatc aattacactg acgtgcattt gcggattgaa
    57181 cgcagctgcc gcttccgtgc tgatccccga cagcaacacg tcggttgtga caatcagtaa
    57241 attagccgta aagccgcaac ctacgcctgc aatggcagcc agtaaacctg caactggatg
    57301 cctgcccacc gccagaaaaa tcagcgcacc catcggtggc atgatcacta acgccgcatc
    57361 ggaagaaatg tggctgaaaa aagcaataaa cagcaccata taactggcgt agcgggcatt
    57421 aacatgcgat gccattttaa ccattagtgc tggcagtaag ccgacgcgct ccgccagacc
    57481 ggcacctaaa accagcgcca ggatcgcacc aagtggagca aaaccgctaa agtttttaat
    57541 aacattgggt aaaaaccagt gtaatccttc cacactgagc aggtttttca ccaccaccgg
    57601 cgtaccatcg gtcgggtttt tcgcactgac gccaaaggcc gacaaaattg ccgtcgtcac
    57661 catgagtacg ataatcaaat agataaagag caaaaaagga tggggaacct tgttaccaat
    57721 tctttcgacc cagccataga gcttcccgga ttgggaggac gacggtatgg atgacatact
    57781 catgggcatt cctcggttgt tgtgtttgcg ttgttgttgt tattttaaag gtgacggtgt
    57841 cacgtttttc gggatagggc agtgatacgg ttgcgtgtcc gtaacttgct gatgctcttg
    57901 ttggcattct tgcaatagcc ctgaatcaat gaagagattg agtgtggttg ctgccatagt
    57961 tttcgccgcc agcagcattc ctttatgagc aatagatgtt cgcccctggc taaccagttg
    58021 ccacgtatgt aggggtgtac cgacggcaaa acaggggctg aaacactggg caacaggcag
    58081 tttccaactg acgtcgccga catcagtcga tgccgcaagc acgttatcgg tggcggcata
    58141 tggagcgact tcattcgcca gtaccgtttc acgatgacgt agtgcaaaaa ccttgccgtt
    58201 ttcgccaccg gttgcggcga tattattcag actgttttgc cgatcgttgg aggtaagcgt
    58261 agcctgaatt tgtttcgcaa aagccagttc ttcggagttc cattccgggg taccaaaatg
    58321 ggatagggcc tggtacatgg cattttctaa ggtgcgattc gggagataac tggaacaggc
    58381 tttgtcgaag cggcattcaa ccgtggtttc ggtcatcaat gccgcacctt cggcgatttt
    58441 ggcgacccga tcataaatat gctgcacgtc ggtcatttcg ggggcgcgga taagataaag
    58501 cacttctgcc tgcgcctgga ccacgttggg cgagatcccg ccactatttg tgatggcata
    58561 gtgtacgcgc gctttttcaa taatatgttc gttgaggaag ttggtgccag tggtcatcaa
    58621 cgttacggca tcaagggcgc tgcgtcccaa atgaggggaa ttcgcggcat gtgctgcgat
    58681 ccctttaaag cgccatgatg cctgaatgtt tgccagcgta cgggtattga acataccggc
    58741 aaaggcttcc gggtgccagg tgagtgccgc atccacatca tcaaataccc cctcgcggac
    58801 catgaacgtt ttacccgagc cgccttcttc gccaggacaa ccataaaagc gcactgtgcc
    58861 gccttgccca tattgttcca gccatttctt gatggctatt gcagcggcaa aggcggcggt
    58921 tcccagcaaa ttgtgtccgc aaccgtgacc attttcaccg ggcgtcacgg atgtaggttg
    58981 cgcgcaacct gcttgctgac ttaaacctgc cagggcgtca tattctccca gcagggcgat
    59041 aaccggtttg ccttgaccaa acgaagcaat aaaggcattt gggatattgc ctacgttgcg
    59101 ggtaacggtg aagcctgcag attccagcgc cgaagccaga tgctccgctg accagaactc
    59161 ttcaaaacgt gtttctggat gatcccagat ttgatcggca atatcggtat aacgttggcg
    59221 atcggcttca atcgcatcgt cgataaaacg atagatttcc tgcatcagat acctcgcgtc
    59281 cagggaaaat tgagcgcggt gcgcgccagc gtttcgacgg caatagcgag aacctgctcg
    59341 tcaaaatcga atttttcgtt gtgatgacct gccgccagct gtgtgccaaa caccacgtag
    59401 gaggcttgcc cttgatgttg ctgcacgcgg gccatcatta atgtggcatc ttcggaaccc
    59461 gcaggcgctt caacacgttc aatggcctga ttgacccccg cgacctgagc cgcctgactt
    59521 tgcaaccatg cgacccattg cggcgaagga gaactggcgg tagctgcacc catcagacga
    59581 gtttcaacgc cgacaccata catggttgct gcgccctgaa tcgcttgctg agcacggtca
    59641 aaaacatatt gattaatgac gtcgctggcc ccgcgtgttt ccactttcag caacgccgag
    59701 gcaggaacaa cgttacgacc gcttcctgcc tgcataacgc ccacgtttac tctggaagct
    59761 ccttcgctgt gcggggcgat tgcatgcagt gcaagagtgg cttgtgctgc cgccaacaag
    59821 gcattgtaac cgtcttctgg ttttgcgcct gcgtgagcgg cggtaccggt gaagtgcgcg
    59881 tcaaatttgg tggttgccat aaaattatca ctgccgcaca ccacggtgcc cgcaggtacg
    59941 ccagtgccaa tgtgcacggc agtaaaataa tcaacatcat ctacgacacc tgcatcgacc
    60001 atcgcccgcg cgccacgcgt accttcctct gcaggctgaa aaatcagttt gatgacgcca
    60061 tgtagtccgg actcgaattg tttaagggta tgcgccagcc caagcccaat ggtggtatgc
    60121 ccatcatgac cacaggcatg catcattccg gcgttacatg acgcaaaacc gtcgcggtag
    60181 gggcgatggc tgacatcctg ctcttcactg agatccagcg cgtccatatc gacacggaaa
    60241 gccatcaccg gaccggggcg accggtatcc agggtggcga cgatacccgt gaaaccacct
    60301 tcaaaagccg caatccattg tgctagcgca ccctgttgac gagcgcgctc gaattcgcgt
    60361 tgtagagtga gttcatcagg tagtcccatc cggctatttt cattaactac ttcgcggccc
    60421 agcgccagtg agtagccgag ctgctgcaat tcttccgcaa caagggtggc agtgcggaat
    60481 tccacccagc cagactctgc atagtgatga aaatcacgtc gccagtgcga taattttggg
    60541 gcaagcgaat taacaaattg attcaaagac tccataacct ttcccgtcat cagtaaaaag
    60601 tgtgacccgg ttcacgtagc gatagttttt acttatcact aactgatttt tcacagtttt
    60661 aaccgttcat aaattaccct gacacaatca tctgcattaa agtagatgcc agtttctttg
    60721 gtctgataaa taacggttat cggtggcgtc atggcttttc aggtaaaaat tcatcaaatt
    60781 cgggcttttg ttgaagtggc tcgtcagggc agcattcgcg gagcgagccg aatgttgaat
    60841 atgtcgcaac cggcactgag taaatctatt caggagctgg aagaagggtt agcggcgcaa
    60901 ctcttttttc gccgtagtaa aggcgtgacg ttaactgatg ccggtgaaag tttttatcag
    60961 cacgccagtc ttattcttga agaactgcgc gcagcccaag aggatattcg ccaacgacaa
    61021 gggcaactgg cagggcagat taatatcggc atgggggcca gtatttcccg cagtctgatg
    61081 ccagctgtaa tatctcgttt tcatcagcag catccgcagg taaaagtacg cattatggaa
    61141 gggcaactgg tgtcgatgat taatgaattg cgtcagggag aattggattt caccatcaat
    61201 acctattatc agggaccgta cgaccacgaa tttacttttg agaaattact ggaaaagcaa
    61261 ttcgcgatct tttgccgccc gggacacccc gccattggtg cccgttcgat caaacagtta
    61321 ctggattaca gctggacaat gccgacgcca cacggcagct actacaaaca gttgagtgaa
    61381 ttgcttgacg atcaggcgca aacgccacag gtcggtgtag tctgcgagac gttctcagcc
    61441 tgtatcagtc tggtggcaaa aagcgatttt ctcagcatac tgcctgaaga aatgggctgc
    61501 gatcccttgc acggacaggg gctggtgatg ttgccggtta gcgaaatttt accgaaagcg
    61561 gcctattatt tgattcagcg gcgtgatagt cgccagacac cactgaccgc gtcattaatc
    61621 acgcaattcc ggcgagaatg cggctatctg caaagttaaa actgcataaa aaaatagagt
    61681 ctgtcgacat ccgccagact ctacagtaca cacagcagtg catccgcgtc ttaaatccgg
    61741 cattgtctcc tctgcgccgg tgactgtgtt ataacggttt taacctttag ttgccaattt
    61801 tcttcagtga cagatttcac ga
//
LOCUS       DBFIFI010000009        59899 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.009, whole genome shotgun sequence.
ACCESSION   DBFIFI010000009 DBFIFI010000000
VERSION     DBFIFI010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 59899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59899
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.009"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..346)
                     /gene="yhaH"
                     /locus_tag="N7B39_000552"
     CDS             complement(<1..346)
                     /gene="yhaH"
                     /locus_tag="N7B39_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HGU2420592.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGP"
     gene            complement(637..1623)
                     /gene="yqjG"
                     /locus_tag="N7B39_000553"
     CDS             complement(637..1623)
                     /gene="yqjG"
                     /locus_tag="N7B39_000553"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HGU2420593.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVEKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTRIISIGPWQDLDEPHGRDVRLG"
     gene            complement(1693..2085)
                     /gene="yqjF"
                     /locus_tag="N7B39_000554"
     CDS             complement(1693..2085)
                     /gene="yqjF"
                     /locus_tag="N7B39_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HGU2420594.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITGYAGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            complement(2271..2570)
                     /gene="yqjK"
                     /locus_tag="N7B39_000555"
     CDS             complement(2271..2570)
                     /gene="yqjK"
                     /locus_tag="N7B39_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HGU2420595.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            complement(2560..2964)
                     /gene="yqjE"
                     /locus_tag="N7B39_000556"
     CDS             complement(2560..2964)
                     /gene="yqjE"
                     /locus_tag="N7B39_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HGU2420596.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            complement(2967..3272)
                     /gene="yqjD"
                     /locus_tag="N7B39_000557"
     CDS             complement(2967..3272)
                     /gene="yqjD"
                     /locus_tag="N7B39_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HGU2420597.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            complement(3310..3678)
                     /gene="yqjC"
                     /locus_tag="N7B39_000558"
     CDS             complement(3310..3678)
                     /gene="yqjC"
                     /locus_tag="N7B39_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HGU2420598.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHQKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            complement(3825..4208)
                     /gene="mzrA"
                     /locus_tag="N7B39_000559"
     CDS             complement(3825..4208)
                     /gene="mzrA"
                     /locus_tag="N7B39_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HGU2420599.1"
                     /translation="MQIPRMSLRQLAWSGAVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPQGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            complement(4212..4874)
                     /gene="yqjA"
                     /locus_tag="N7B39_000560"
     CDS             complement(4212..4874)
                     /gene="yqjA"
                     /locus_tag="N7B39_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HGU2420600.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            complement(4896..4979)
                     /gene="yqiM"
                     /locus_tag="N7B39_000561"
     CDS             complement(4896..4979)
                     /gene="yqiM"
                     /locus_tag="N7B39_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HGU2420601.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            complement(5219..5995)
                     /gene="exuR"
                     /locus_tag="N7B39_000562"
     CDS             complement(5219..5995)
                     /gene="exuR"
                     /locus_tag="N7B39_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HGU2420602.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            complement(6125..7423)
                     /gene="exuT"
                     /locus_tag="N7B39_000563"
     CDS             complement(6125..7423)
                     /gene="exuT"
                     /locus_tag="N7B39_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HGU2420603.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            7906..9318
                     /gene="uxaC"
                     /locus_tag="N7B39_000564"
     CDS             7906..9318
                     /gene="uxaC"
                     /locus_tag="N7B39_000564"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HGU2420604.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMSGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            9333..10820
                     /gene="uxaA"
                     /locus_tag="N7B39_000565"
     CDS             9333..10820
                     /gene="uxaA"
                     /locus_tag="N7B39_000565"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HGU2420605.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVRLRQDVARGHKF
                     ALTNIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            10903..11454
                     /gene="ygjV"
                     /locus_tag="N7B39_000566"
     CDS             10903..11454
                     /gene="ygjV"
                     /locus_tag="N7B39_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HGU2420606.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(11459..12703)
                     /gene="sstT"
                     /locus_tag="N7B39_000567"
     CDS             complement(11459..12703)
                     /gene="sstT"
                     /locus_tag="N7B39_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HGU2420607.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(13102..14067)
                     /gene="alx"
                     /locus_tag="N7B39_000568"
     CDS             complement(13102..14067)
                     /gene="alx"
                     /locus_tag="N7B39_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HGU2420608.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQQVG"
     gene            complement(14351..15337)
                     /gene="ygjR"
                     /locus_tag="N7B39_000569"
     CDS             complement(14351..15337)
                     /gene="ygjR"
                     /locus_tag="N7B39_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HGU2420609.1"
                     /translation="MIRFAVIGTNWITHQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     EFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(15416..16108)
                     /gene="ygjQ"
                     /locus_tag="N7B39_000570"
     CDS             complement(15416..16108)
                     /gene="ygjQ"
                     /locus_tag="N7B39_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HGU2420610.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWSDVNVVPARNVGLLLGARPGNRYFTRRIDTAAELYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRANKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLEKGRGKIVRLREKLARVSAVIDAKIFNRQPKYLGPSVII
                     GPFSEHGCPAQK"
     gene            complement(16185..16688)
                     /gene="ygjP"
                     /locus_tag="N7B39_000571"
     CDS             complement(16185..16688)
                     /gene="ygjP"
                     /locus_tag="N7B39_000571"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HGU2420611.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMDPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            16773..17909
                     /gene="rlmG"
                     /locus_tag="N7B39_000572"
     CDS             16773..17909
                     /gene="rlmG"
                     /locus_tag="N7B39_000572"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HGU2420612.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTPQTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            18193..18507
                     /gene="higB"
                     /locus_tag="N7B39_000573"
     CDS             18193..18507
                     /gene="higB"
                     /locus_tag="N7B39_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HGU2420613.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            18504..18920
                     /gene="higA"
                     /locus_tag="N7B39_000574"
     CDS             18504..18920
                     /gene="higA"
                     /locus_tag="N7B39_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HGU2420614.1"
                     /translation="MIAIADILHAGEKLTAVAPFLAGIQNEEQYIQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(18965..20983)
                     /gene="fadH"
                     /locus_tag="N7B39_000575"
     CDS             complement(18965..20983)
                     /gene="fadH"
                     /locus_tag="N7B39_000575"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HGU2420615.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYHNRMRFAVEVVSAVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAMQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRTPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIAGFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(21509..23860)
                     /gene="ygjK"
                     /locus_tag="N7B39_000576"
     CDS             complement(21509..23860)
                     /gene="ygjK"
                     /locus_tag="N7B39_000576"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HGU2420616.1"
                     /translation="MKIKTILTPVTCALLISFSAHATNADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYNIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTSQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKTWVAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGNKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVASGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPATQ
                     YFYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHFYMLYNDFFRKQ"
     gene            complement(23877..24947)
                     /gene="ygjJ"
                     /locus_tag="N7B39_000577"
     CDS             complement(23877..24947)
                     /gene="ygjJ"
                     /locus_tag="N7B39_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HGU2420617.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASNDPSDQIFLMQYFYW"
     gene            complement(25081..26514)
                     /gene="ygjI"
                     /locus_tag="N7B39_000578"
     CDS             complement(25081..26514)
                     /gene="ygjI"
                     /locus_tag="N7B39_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HGU2420618.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(26577..27026)
                     /gene="ebgC"
                     /locus_tag="N7B39_000579"
     CDS             complement(26577..27026)
                     /gene="ebgC"
                     /locus_tag="N7B39_000579"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HGU2420619.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVDEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(27023..30115)
                     /gene="ebgA"
                     /locus_tag="N7B39_000580"
     CDS             complement(27023..30115)
                     /gene="ebgA"
                     /locus_tag="N7B39_000580"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HGU2420620.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKT
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAIFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            complement(30299..31282)
                     /gene="ebgR"
                     /locus_tag="N7B39_000581"
     CDS             complement(30299..31282)
                     /gene="ebgR"
                     /locus_tag="N7B39_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HGU2420621.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            31501..31833
                     /gene="ygjH"
                     /locus_tag="N7B39_000582"
     CDS             31501..31833
                     /gene="ygjH"
                     /locus_tag="N7B39_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HGU2420622.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRIV"
     gene            complement(31875..33254)
                     /gene="ygjG"
                     /locus_tag="N7B39_000583"
     CDS             complement(31875..33254)
                     /gene="ygjG"
                     /locus_tag="N7B39_000583"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HGU2420623.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            33672..35192
                     /gene="aer"
                     /locus_tag="N7B39_000584"
     CDS             33672..35192
                     /gene="aer"
                     /locus_tag="N7B39_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HGU2420624.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTGKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYILCALVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            complement(35346..35969)
                     /gene="nfeR"
                     /locus_tag="N7B39_000585"
                     /gene_synonym="yqjI"
     CDS             complement(35346..35969)
                     /gene="nfeR"
                     /locus_tag="N7B39_000585"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HGU2420625.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLNLRVNQSDITDAQIKKIIAVIDRAAFDITQLD"
     gene            36257..37021
                     /gene="nfeF"
                     /locus_tag="N7B39_000586"
                     /gene_synonym="yqjH"
     CDS             36257..37021
                     /gene="nfeF"
                     /locus_tag="N7B39_000586"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HGU2420626.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            complement(37075..37150)
                     /locus_tag="N7B39_000587"
     tRNA            complement(37075..37150)
                     /locus_tag="N7B39_000587"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37115..37117),aa:Ile,seq:cat)
     gene            37275..37781
                     /gene="mug"
                     /locus_tag="N7B39_000588"
     CDS             37275..37781
                     /gene="mug"
                     /locus_tag="N7B39_000588"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HGU2420627.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDHQLKPQEAQHLLDYRCGVTKLVDRPTVQANEISKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLSIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            complement(37860..39701)
                     /gene="rpoD"
                     /locus_tag="N7B39_000589"
     CDS             complement(37860..39701)
                     /gene="rpoD"
                     /locus_tag="N7B39_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HGU2420628.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(39896..41641)
                     /gene="dnaG"
                     /locus_tag="N7B39_000590"
     CDS             complement(39896..41641)
                     /gene="dnaG"
                     /locus_tag="N7B39_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HGU2420629.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            complement(41752..41967)
                     /gene="rpsU"
                     /locus_tag="N7B39_000591"
     CDS             complement(41752..41967)
                     /gene="rpsU"
                     /locus_tag="N7B39_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HGU2420630.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            42205..43218
                     /gene="tsaD"
                     /locus_tag="N7B39_000592"
     CDS             42205..43218
                     /gene="tsaD"
                     /locus_tag="N7B39_000592"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HGU2420631.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            complement(43261..44724)
                     /gene="ttdT"
                     /locus_tag="N7B39_000593"
     CDS             complement(43261..44724)
                     /gene="ttdT"
                     /locus_tag="N7B39_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HGU2420632.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(44773..45378)
                     /gene="ttdB"
                     /locus_tag="N7B39_000594"
     CDS             complement(44773..45378)
                     /gene="ttdB"
                     /locus_tag="N7B39_000594"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HGU2420633.1"
                     /translation="MKKILTTPIKAEDLQDIHVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPLPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            complement(45375..46286)
                     /gene="ttdA"
                     /locus_tag="N7B39_000595"
     CDS             complement(45375..46286)
                     /gene="ttdA"
                     /locus_tag="N7B39_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HGU2420634.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATIKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIVPDGDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            46493..47425
                     /gene="ttdR"
                     /locus_tag="N7B39_000596"
                     /gene_synonym="dan"
     CDS             46493..47425
                     /gene="ttdR"
                     /locus_tag="N7B39_000596"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HGU2420635.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVYFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            complement(47438..48055)
                     /gene="plsY"
                     /locus_tag="N7B39_000597"
     CDS             complement(47438..48055)
                     /gene="plsY"
                     /locus_tag="N7B39_000597"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HGU2420636.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            48161..48529
                     /gene="folB"
                     /locus_tag="N7B39_000598"
     CDS             48161..48529
                     /gene="folB"
                     /locus_tag="N7B39_000598"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HGU2420637.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DNVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            48620..49441
                     /gene="bacA"
                     /locus_tag="N7B39_000599"
     CDS             48620..49441
                     /gene="bacA"
                     /locus_tag="N7B39_000599"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HGU2420638.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTSGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(49622..50860)
                     /gene="cca"
                     /locus_tag="N7B39_000600"
     CDS             complement(49622..50860)
                     /gene="cca"
                     /locus_tag="N7B39_000600"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HGU2420639.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETENALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFTTLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPE"
     gene            complement(50924..51544)
                     /gene="ygiM"
                     /locus_tag="N7B39_000601"
     CDS             complement(50924..51544)
                     /gene="ygiM"
                     /locus_tag="N7B39_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HGU2420640.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            51786..53087
                     /gene="ygiF"
                     /locus_tag="N7B39_000602"
     CDS             51786..53087
                     /gene="ygiF"
                     /locus_tag="N7B39_000602"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HGU2420641.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLHHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            53110..55950
                     /gene="glnE"
                     /locus_tag="N7B39_000603"
     CDS             53110..55950
                     /gene="glnE"
                     /locus_tag="N7B39_000603"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HGU2420642.1"
                     /translation="MKPLSSPLQQYWQTIVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SISAHPEWLTELESQPPQADEWQHYVAWLQEALINVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRWELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLLRITALLAGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSTEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVPEDCFTAERELVRASWQKWLVEE"
     gene            55998..57431
                     /gene="hldE"
                     /locus_tag="N7B39_000604"
     CDS             55998..57431
                     /gene="hldE"
                     /locus_tag="N7B39_000604"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HGU2420643.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPSSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVVA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(57551..57610)
                     /gene="ibsE"
                     /locus_tag="N7B39_000605"
     CDS             complement(57551..57610)
                     /gene="ibsE"
                     /locus_tag="N7B39_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HGU2420644.1"
                     /translation="MMKLVIILVVLLLLSFPTY"
     gene            complement(57927..57986)
                     /locus_tag="N7B39_000606"
     CDS             complement(57927..57986)
                     /locus_tag="N7B39_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HGU2420645.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            complement(58225..59886)
                     /gene="yqiK"
                     /locus_tag="N7B39_000607"
     CDS             complement(58225..59886)
                     /gene="yqiK"
                     /locus_tag="N7B39_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HGU2420646.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLKIEQQEAFMTLEQEQQVKTRTAEQNAKIAAFEA
                     ERRREAEQTRILAERQIQETEIDREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGGTAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSLLTPTTPVAENVE"
BASE COUNT        14191 a        15536 c        15934 g        14238 t
ORIGIN      
        1 caggtccaaa gcggttttcg cctggcgtac ccgcctggca gttaaagacg atgataataa
       61 gccagccgat aaacgggatt aagaatagca gtgcccacca cgccgagcgg tcggtgtcgt
      121 gcaggcggcg gaactgaact gcccaccacg gtaaaaacac cagaataccg tagattgtcg
      181 tcaggatgcc ttcgccacca gcgcgttgcc agcctaacat tttatccagc agccccagca
      241 cgaacgtaaa gatgatgttg accagaataa acatccagta ctctttgcgc cgcgcacgac
      301 cccggaaacc gacataattt tttagtactt tcagatacca gtccattttt gcctcaattt
      361 tgctcaatca cttgttttta aagcagcaaa ttaagaatag acgtgaatga aatggcagta
      421 aatattcgtt caaggaatta ataaagtgct gtgaaggcgc gctaagactg atgacaagcg
      481 caaaattgcc agatgcgcta cgcttatctg gcccgcgcgg tcattgcaac atattgaata
      541 taaaagtttt gtaggtttga taaggcgttc atgccgcatc cgacatgaac aacatgcact
      601 tagctaatta tcagaggcac ctcgtaaggt gcttttttaa cctaagcgaa catctcgtcc
      661 atgcggttca tcgagatcct gccacggacc aattgaaata atccgcgtag ggttgatggt
      721 cttatgactg cggaagtaat gattacggat atgatcgaaa ttgactgttt cggcaattcc
      781 cggcatctgg tagatatcac gcaggaagcc atacagatta aggtaatcgc tgatgcggtg
      841 cttatcacac ttgaagtggg tcacatacac tggatcaaaa cggaccagcg tggtccacag
      901 gcgaatatcg gcttcggtta gctggttgcc ggtcaggtaa cgatgctgac ctaaaatctg
      961 ttccagtcgc gccagcgatt caaacacttt ctccaccgct tcgtcgtagg cttgctggct
     1021 ggtggcaaaa ccagctttat acacgccgtt gttaacggtg tcataaatcc agccgttaag
     1081 ttcgtcaatt ttcgtttgca gggctggtgg gtagtaatca cccgctttcg cgcccagcgc
     1141 atcaaacgcg gtattaaaca tgcggatgat ttctgctgat tcgttgctga cgatggtgtg
     1201 gttctttttg tcccacagca cgggaacagt aactcgtccg ctgtagtgtg gatcggcgtg
     1261 gagataaagc tgatacaaaa attcatgttg atagagcgtg tctccggttg ctcccggaaa
     1321 actgtcatca aaggtccagc cgttttccag catcagcggg ttcactacgg aaacggaaat
     1381 aaacggttcc agtcctttga gtttgcgcat gatcagcgtg cggtgcgccc acgggcaggc
     1441 gagtgaaaca tagagatgat aacgatcttt ctctgcgata aaaccgcctg tgccagtggg
     1501 gccaggagcg ccatcggcag tgagccagtt acgaaatgcg gaagctgaac gttgaaattt
     1561 accgccggta gatttggtat cgtaccaggt gtcatgccag acgccgtcaa tcagttgacc
     1621 cattgttctc tcctccaata gcaaaaacga ggagcttcct cctcgttttt tatcgttaag
     1681 tatagcgcgt gcttaccact ttttattcag caggcggtcg atgctatacg cgcccggacc
     1741 ggtaattgcc agcagcagga atccgccaga aattgtcagg tttttcatga acatcagcga
     1801 gttgacgcct tcagcaaagt tgctgtgaaa taaaaatgcc gtcagcagcg taaagcccgc
     1861 agtaaacagg gctgtggtgc gagtcaggaa accgaacagg attgccagac caccaccaaa
     1921 ctcaagcaga atcaccagcg gcagcataaa acccgggacg cccatcgctt ccatatattg
     1981 ttgggtaccc gcgtaaccag taatttttcc ccagcctgcg gtaataaaca gaatcggcat
     2041 taaaatgcgc gctaccagta caccaacatc ttctaatttt ttcatcatac tctccactgc
     2101 gccactcagg ctgctgattg tttttttgtg caagggcgcg gtattagcgt cgttgctgtc
     2161 gatggagaga atcataaacg tggtgaatga tgattgttag caaggaaaac tgtcaaaaat
     2221 cttcaaaaaa tttgatgggt aaggccggaa tggctccggc cagagggaag ttaaccgcga
     2281 agctgttgct gcttgagggt cgttttaacc agacgccagg cgctccatac gccaaaaccg
     2341 cgtctggccc agcggaccag catattagga tggcgaatcg tccagatcgc catcacgcta
     2401 ctgccaacca gcgcccagga gcgcagactt agcagcatat tccagcgacg atcgtaagcg
     2461 cctgttgcct ccagccattc acgacgactg gcagaaagat ccagccgttg ttgctggatc
     2521 tggctaagca gttgcgcctt acgtcgttca cgttcgactt tactgctcac gggactcctc
     2581 ctcgagcagc tgccgatcgt ttgccagctc atggcgtgta tggcgcagca aggtggattt
     2641 acgcgattta cgtaacgtcc agataccgcc aatcagtgcc agtagcagca acaccacggt
     2701 ggtggcaatc atcgcattca ggcgatattg cggatcaacc gcccaaataa ttagcaccat
     2761 caggctcata agaccaaatg cagcgaaaag catcgtcagg cccagcatca gtaaaagttg
     2821 aaagagatta gctttttcct cttccagctc aaccaccgcc agccgcagac gtgtttccac
     2881 catttcaacc atgatggaaa caattcgctg cccgatgccc agaacgcttt taccgggccc
     2941 ttgcgcgtga tgagtgtccg ccataattaa cgacgcgaca gcagaacgcc gagcactaca
     3001 ccgattgcag cgccaatgcc cacgcccgtc catggatttt cacgcacata ctcatcggca
     3061 cgcgccgccg cgacacgggt ttgtttggcg atggcatcac cggtttcacc caggcgataa
     3121 cggctctgtt tcagtgcctg ctccgctttg ctacgaatct tactcaactc ttctttcgac
     3181 ttctcgccag atgagctaag cacctcttcc agcgtatcgg aaagggattt caactcagca
     3241 cgcagatgtt ccgtagtgtg ttctttcgac atagttttct ccaggtgagt aagtagtgac
     3301 tattgtgagt tagtagtcgc gcgcttccag ctttttcagc tcttcctgcg cttctgccag
     3361 tttccgttcg cgtttggcaa tcttatcggc atcgcctttt tgcttcgcct cggctaaatc
     3421 ttgctggcgt tccgccacct catctttctg ctttgcgatt ttcttctgat gatcggcacg
     3481 cagctggcta tctgaacagt tggcccggac ttcactcagg gctttattca gaccgtcaat
     3541 acgattctgg ttttggtgtt tttcggcata gctgatctcc ttaaggatat tttgctcctt
     3601 ttcctgacac agggtagtgg cataactacc ggcactaaga gcaaaaagag aaacagctaa
     3661 agcgatgcgg tatttcattc ttgaaccttc cattgtgatt cggacctatt acaaaggaac
     3721 gattgtccag acaacgaagc ggtagtgtta accaccgcct cattaatcat agacagttac
     3781 ggtgaaatca ccaaagtgag tgatctgttt cggacaacag gaagttatcc gaagcgatga
     3841 gagttatccc gtagccgggt cagccactgc atagcctgac tgttattgtc ctgctgcgca
     3901 atgatgtagc cttggggcaa tgttctgtcg aggaccgctt ttgcagcggc gctctggtcg
     3961 ctggagtcaa aggtaattaa cagagtgtcg tttttggggg tgatactttt gaaaggaatg
     4021 ccatgagcgt caaggtgatg ccagattgaa aaaccgtctg gcatcgttgt gccttgatga
     4081 acggcacgaa tcgccagcgt agactcttgc tggcgaaccg cggaccaggc cagcaacagc
     4141 gtgccgacca gaagtaaaac agcgccggac caggctagct ggcgaagcga catgcgaggt
     4201 atttgcatcc cttacccccg atttccatat ttctttttcc ataatacgac cagagaacct
     4261 gccaggccaa acaccagcag caccaccggg agcagcatca ggcatgacat cagctggtcc
     4321 tcgtacttta aaaataccgg cgttttgccg agcatgtaac ccagagttgt caggatcaat
     4381 acccacagca gaccgctcat ccagttgaaa aactgaaagc gcgcgttatt cagccctgat
     4441 aacccggcaa tcgtcggcag cagtgttctg acaaacgcaa taaagcgacc aattaacagc
     4501 gccgataaac cgtgtttatg aaaaagatgg tgtgcgcgtt gatgataatg cgcgggtaaa
     4561 tgagatagcc agttttgtac ggtgcgggta ttgcccagcc atcgcccctg aatatagctg
     4621 acccagcagc cgaggctggc ggcaacggtc agcagcagaa tcgtttgcgg atagcccatc
     4681 gcgcctttcg caatcaacac gccgaccaat accagtaaac tgtcgcccgg taaaaaggcc
     4741 gccggaagca agccgttttc aaggaacaaa attacaaaca agacaaaata caacatgcca
     4801 atcatcgatg gattggccag ggtttcaaaa tcctgcgccc acagggcttg cagcaattgg
     4861 gtcaaaagtt ccattcatta ttcctggtga atcggttaac gccacgctgg ttttaaaaga
     4921 tgcactaccg caatgttatt gctgttttgg tatgttctgc gggtttgata catcaacaca
     4981 gcctgaacta ttccctgtta aatcgtgaga taagaaagca ttcaaaaaca taaattcaaa
     5041 ttgaatctaa ttgtaacaaa ggtcaacgtt gttcgttatc aaaattacat gtccgtggag
     5101 tctgattttg ctgaatgcag gggagggtgg cgagaggatt tacacaggct gacactttat
     5161 acttttcgct taccgacgag acaaacaggc gcgtcgcagc gcctgtcgga caggtatttc
     5221 atttactgcc actggtggcc gtatcgagat gcactaccgg gttttcggcg aacagatagc
     5281 ggtcggcatt gaactcgaag tcgtcgctgg tttcgttgaa taacatgatc ttggtgtttt
     5341 ccaggtgctg ccacattgcc agcttagcag catgaggatc tttgcgaatc agcgccttga
     5401 ggatttgatc gtggtcatca caccagttat cgacggtacg ggaatcaatg tgttcgtgca
     5461 gttttttcca gtacgggtta tggctacgct gggtccacat tttttcaacg atagccgcca
     5521 gggcggagtt ctgcgtcgcc agggctacct gaatatggaa ctgcaaatcc cactcggaat
     5581 cacggaagca ttgttcgccg cgcgcctgct cctggatggc catcaatttc atgatgtcct
     5641 gtttcgttac ctgagttgct gcgaactcgg caatattact ttcgatgagt tggcgagcct
     5701 ggagtaactc aaacggaccg taattggcga attccatatt attgtccgca gcctgctgat
     5761 ggcgcggctg gttggaaacc acgtgaatac ctgaaccttt acgcacttcg acatagcctt
     5821 caacttccag catgatgatg gcttcacgaa ctaccgtacg gctgacgttc ttttcatcgg
     5881 caataaagcg ctcagcaggc agtttatcac ccaccagata gacgccctgt tcgatgcgct
     5941 ctttcaggtc agcggcaagt tgttgataca aacggcgtgg ttcagtgatt tccatatgcg
     6001 ctccaggcat gatacggcag actatatttg ttataccact tttgcgagtt tagctctact
     6061 cgcagtgatg aaaaagccgc ctgagaaggc ggctttgaag cgaaactaaa acttacacga
     6121 agcgttaatg ttgcggtgcg ggatcgttat gtgtttcctg cgccacctca atcgccggtt
     6181 tgttctgcaa tacggtccag ataaccagcg cacccagcag gtcaaatact gccagaactg
     6241 cgaacagcgg gctgaagccg atggtgtcag ccagtgcacc gacaaccagc gcaaacagcg
     6301 tacttgccag ccatgcggac atcccggtta aaccgttcgc cgttgccact tcgttacgac
     6361 caaacacgtc ggaagagagc gtaatcagcg cgccagacag tgcctggtgg gcaaaaccac
     6421 cgatacacag cagcataatt gcgacatacg ggttggtgaa caggccgatc atacccgggc
     6481 caatcatcag cactgcaccc agcgttacga ccatcttacg ggaaacgatc aggttcacac
     6541 caaaccaacg ctggaacagc ggcggcaggt aaccaccgag gatacaaccg aggtcagcaa
     6601 acagcatcgg catccaggcg aacatcgcga tctctttcag gttaaagccg taaactttaa
     6661 acatgaacag cgggatccac gcgttaaaag taccccaggc cggttctgcc aggaaacgcg
     6721 gcagcgcgat accccagaac tgacggttac gcaggatctg accaacggac attttcttcg
     6781 ccgtactcac ctggtgctgg gcttcctgac cattaataat atagtcgcgt tcttcatcgg
     6841 tcagatgctt ctggtcgcgc ggatgtttat agaaaatcag ccatgccatc gcccagataa
     6901 agctcaatgc accggagatg ataaatgcca tctgccagct gtgcatcacg attgcccata
     6961 ccaccagcgg cggcgcaatc atcgcaccaa tcgaagaacc tacgttaaaa tagcctactg
     7021 cgatggaacg ctctttcgcc gggaaccatt cggagctggc tttcagaccc gccgggatca
     7081 tcgctgcttc cgcggcaccg accgcaccac gagcaacagc caggccaccc cagctacctg
     7141 ccagcgcggt tgcaccacag aacacggccc acagcacagc aaacattgca taaccgattt
     7201 tcgtacccag cacatccagt acataacctg ctaccggttg catgaccgta taagcagcag
     7261 aataggctgc gatgatatag gaatactgtt gggtggagat gtttaactct tccatcagag
     7321 ttggcgcagc tgccgccaca gtgttacgcg tcaggtaacc aagcacggtg ccgagcgtca
     7381 ccagtgcgat catataccaa cgtaaccctt taattttacg catgtaaaac ctcatcgttt
     7441 tgttatatcc gcaggaagcg cggccatcat cccgccagag gctgaagatg ttagttacga
     7501 ccgggtaacc gaaaaaaaac cggcacgccc cgaacgttgc catgaaagtt tcgtgctaat
     7561 tcggcttccg taccggttaa cccgattcat ctaacaaatg aatgaatcgg caatttgcat
     7621 tccgtcgact tatgatttgc gacggcagaa agataacttg tcatacaact ttaaaaggtg
     7681 agagccatca caaatgtgcg aatatttgta gggacattac ctgacgacag cgaggccagt
     7741 actggcgcgg cctgcagaga gatttaccac tttgagagta atttttttaa ctacgtttat
     7801 tgatctaact cacgaaaata tcttcggact ctggaaattg gtgtgataac tttgtcagca
     7861 tcgcaccata agcaagctag ctcactcgtt gagaggaaga cgaaaatgac tccgtttatg
     7921 actgaagatt tcctgttaga taccgaattt gcccgccgtc tgtatcacga ctacgcaaaa
     7981 gaccagccga ttttcgatta ccattgccat ttgccgccgc agcagattgc ggaagactat
     8041 cgttttaaaa acctgtatga catctggctg aaaggcgatc actacaaatg gcgcgctatg
     8101 cgtaccaacg gtgtggccga gcgtctgtgt accggtgatg cgtctgaccg tgaaaaattt
     8161 gacgcctggg cggcgactgt tccgcacact atcggcaacc cgttatacca ctggacgcac
     8221 ctcgaactgc gtcgtccgtt tggtatcact ggcaaattgc tttctccgtc aactgccgat
     8281 gaaatctgga acgaatgtaa cgaactgctg gcgcaggata acttctccgc gcgcggcatc
     8341 atgcagcaga tgaacgtgaa aatggtcggc accaccgatg acccgatcga ttctctggag
     8401 catcacgcag agatcgccaa agacggctct ttcaccatca aagtgctgcc gagctggcgt
     8461 ccggacaaag ccttcaacat cgaacaggcg acctttaacg actacatggc gaagctgggc
     8521 gaagtttccg ataccgacat tcgccgcttt gctgacctgc aaactgcctt gaccaaacgt
     8581 ctggatcact tcgccgctca cggctgtaaa gtgtctgacc atgcgctgga cgtggtgatg
     8641 tttgctgaag cgaacgaagc ggaattggac agcattctgg cgcgccgtct ggctggcgaa
     8701 accctgagcg agcacgaagt ggcacagttc aaaactgcgg tactggtgtt ccttggtgcc
     8761 gaatatgcac gtcgcggctg ggtacagcag taccacattg gcgcgctgcg taataacaac
     8821 ctgcgtcagt tcaaactgct ggggccggat gtaggctttg actccatcaa cgaccgtccg
     8881 atggcagaag agttgtctaa gctgctgagc aagcagaacg aagaaaacct gctgccgaaa
     8941 accattctgt actgcctgaa cccgcgcgat aacgaagtgc tgggcaccat gagcggtaac
     9001 ttccagggcg aaggtatgcc gggcaaaatg cagttcggtt ccggctggtg gtttaacgat
     9061 cagaaagacg gtatggaacg tcagatgacc caactggcgc agctcggttt gctgagccgc
     9121 tttgtcggta tgctgactga cagccgtagc ttcctgtcat acacccgtca cgaatacttc
     9181 cgccgcattc tgtgccagat gatcggtcgc tgggtggaag cgggcgaagc accggcggac
     9241 atcaacctgc tgggcgagat ggtgaaaaat atttgcttta acaatgcgcg tgactacttc
     9301 gccattgaac tgaactaagg tctggggttg atatgcaata catcaagatc catgcgctgg
     9361 ataacgtcgc ggtcgcttta gcggatttgg ctgaaggcac agaagtcagt gtcgataacc
     9421 aaactgttag gctgcgccag gatgttgctc gtggacataa atttgcgtta acgaatatcg
     9481 caaaaggggc caacgtcatc aaatatggcc tgccgattgg ttatgcattg gcggatattg
     9541 cggcggggga acacgttcac gcccacaata cgcgcacgaa tctgagcgat ctggatcagt
     9601 atcgctatca acctgatttt caggatctgc ctgcgcaagc ggcagatcgt gaagtgcaga
     9661 tctatcgtcg cgctaacggc gatgtcgggg tgcgtaacga gctgtggatc ctgccaaccg
     9721 tgggctgtgt caacggcatc gcgcggcaga tccagaaccg ttttctgaaa gagaccaaca
     9781 acgctgaagg taccgacggc gtgttcctct tcagccacac ctacggctgc tcacagctgg
     9841 gcgacgatca cattaatacc cgcaccatgc tgcaaaacat ggtgcgccac ccgaacgcag
     9901 gcgcagtgct ggtgattggt ctgggctgtg aaaacaacca ggttgccgca ttccgtgaaa
     9961 cgctgggcga tatcgatcct gaacgcgttc atttcatgat ctgccaacag caggatgatg
    10021 agatcgaagc cggaatcgag catttgcatc agctgtataa cgtgatgcgc aacgataaac
    10081 gcgagccagg caaactcagc gaactgaagt ttggtctgga gtgcggtggt tctgacggtc
    10141 tttctggtat tactgctaac ccgatgctgg ggcgtttctc tgactacgtg attgctaacg
    10201 gcggtactac cgtactgacc gaagtgccgg agatgtttgg cgcagagcag ttgctgatgg
    10261 accattgccg cgacgaagca acgtttgaaa aactggtcac catggtcaac gacttcaaac
    10321 agtactttat tgcccatgac cagccgatct atgaaaaccc atcgccgggg aacaaagcgg
    10381 gcggtatcac cacgctggaa gataaatccc ttggctgtac ccagaaagcg ggttccagcg
    10441 tcgtggttga cgtgctgcgt tacggcgagc gtctgaaaac gccggggctg aacttgttaa
    10501 gtgcgccggg taacgatgcc gtagcgacca gcgccctggc gggtgcgggc tgccatatgg
    10561 tgctgttcag tactggtcgt ggcacgccat acggcggctt tgtaccgacg gtgaaaatcg
    10621 ctaccaacag tgaactggcg gcgaagaaaa aacactggat cgactttgac gcgggtcagc
    10681 tgatccacgg taaagcgatg ccgcagttgc tggaagaatt tatcgatacc atcgttgagt
    10741 ttgccaacgg taaacaaacc tgcaacgagc gtaacgactt ccgcgaactg gcgatcttca
    10801 aaagcggcgt aacgctataa agtcgaaatg agttgttaat aaaaaacggc gtttcataat
    10861 gtgaagcgcc gtttgctttt ccatacctga taaggattta tcatgaccgc gtattggctg
    10921 gcccagggcg tgggtgtcat cgcctttctg attggtatca caacattttt caatcgtgac
    10981 gaacgtcgct tcaaaaagca gctttcggtc tatagcgccg ttattggcgt acattttttt
    11041 cttctgggca cctatcccgc tggtgccagt gccatcctta atgccattcg tacattgatt
    11101 actttacgca cgcgcagctt atgggtaatg gcgattttta ttgtgctgac tggcggaatt
    11161 ggcctcgcga agttccatca tcctgtcgaa ctattgccgg ttatcggtac gattgtcagt
    11221 acctgggcgc tgttccgctg taaagggctc accatgcgct gcgtgatgtg gttttcaacg
    11281 tgttgctggg tgattcacaa cttctgggcg gggtcgatag gcggcacgat gattgagggt
    11341 agttttctgc ttatgaatgg cctgaatatc attcgtttct ggcggatgca gaaaagggga
    11401 attgatccgt ttaaagtaga gaaaacccct cccgccatag acgaaagggg ttaaacaatt
    11461 aattacgcag ggcgctattt gccagacgat cgtcttccgc ctggcaagct gccgcagtga
    11521 acagcacgtc agttgaagag ttcagcgcgg tttcgcaaga gtcctgcaat acgccgataa
    11581 taaagccgac ggcaaccacc tgcatggcga tatcgttcga aataccgaac atattacagg
    11641 ccagtgggat cagcagcaaa gaaccccccg ccacgccgga tgcgccacag gcacacagag
    11701 aagccactac gctcaacagc agcgctgtgg gcagatcgac cggaataccc agcgtattaa
    11761 ccgcagccag cgtcaacacg gtaatggtga ttgctgcgcc cgccatattg atggtggctc
    11821 ccagcggaat agaaacggaa taggtatcgc gatccagatt cagcttttca cacagtgcca
    11881 tattcaccgg aatgttcgcc gcagagctgc gggtgaagaa ggcatacacg ccgctttcgc
    11941 gcaggcacag cagcaccagc gggaacgggt tacgacgaat tttccaccac accagcaatg
    12001 ggttaaccac cagcgccacc agtaacatac agccaaccag cacgaccagc agttgcgcgt
    12061 agccccacag tgtggagaaa ccggtggttg ccagggtaga agaaaccaga ccaaaaatac
    12121 caatcggtgc gaagcgaatg accagtttca ccataaaggt gacggcattc gacatatcgt
    12181 tgaccaggtt tttggtggtc tcgttaccgt gacgcagtgc gaaaccgagg ccaattgccc
    12241 acaccaggat cccgatgtag ttacctttca gcagcgcgtc gatggggttg gaaaccatgc
    12301 tcattaccag cccgcgcatc acttctacaa tgcctgacgg tggcgaaata tcacccgcgc
    12361 tactggataa gtgcagggta gaagggaagg cgaagctgaa aacgactgcg gccagagcag
    12421 cagagaaggt acccagcaga tagaggaaca aaatagggcg gatattggtt ttctgcccgt
    12481 gctggtggtt agcaatagat gccatcacca gcatcaacac caggatgggg gcaacggctt
    12541 tcagtgcgcc gacgaacaaa gtacctaaca gaccaacagc ttccgccgcg ggttttgaga
    12601 tccatgccag aagaatcccc agaacaaggc cgaccaggat ttgttttacc aggctgccat
    12661 gagccagacg ccggaatagc cccggtgaac gttgcgtagt catttttcga tcctttcatt
    12721 gtgttgtcgc acatccctgg tgcgttctgt cgacgcatct ttcaggatgt aacaaagtgt
    12781 ttgcaaggtc gagtataagg aaagttacag cttcgggaag cgaaaaatgc tggattttaa
    12841 agagaggtca tattttgcaa cttaatggtt acatttattt gacataaaaa taaagccaac
    12901 gtgcggcttc cagactgagg actacgtcaa aaaaggactc ggtcagtccc ctcgccccgt
    12961 ttggggagag ggttagggtg aggggaacag gccagcatcg gtgcgaaaaa ttaccctcac
    13021 cccgaccctc tccctggaag ggagagggag aaaacggtgc tcatgttgat gacagggtac
    13081 acggcttagg cagattaaaa atcatccaac ctgctgctta tcatgccgat aattcaccca
    13141 ggcgttgata ataaacgtca tcaccaggat gccaaacacc acgcccagcg agacggcgat
    13201 tggaatatgg tagaagtcga caatcagcat cttgataccg ataaacacca gaatcaccgc
    13261 caggccatat ttgagcatcg agaaacgctc tgctacgccc gccagcagga aatacatcgc
    13321 acgcaggccg aggatcgcaa acaggtttga ggtcagcaca atgaacgggt cagtggtcac
    13381 ggcgaagata gccggaatgc tatccacggc aaaaatcacg tcgctcaatt ccaccagaat
    13441 cagtaccagc atcagcggtg tggcgtacag caatccattc ttacgcacaa agaaatgctc
    13501 gttgtcgatg gtgtcggtca tgcgcaaatg accgcgtagc cagcgcacca gcggcttgtc
    13561 gccaatgcct gattcatctt catgggcgag cgccatcttc acgccggtaa acagcaggaa
    13621 ggcaccgaag atatacagga tccagtcgaa ctgcgaaatc agccagctgc cagtgaagat
    13681 catgatggta cgcagaacaa tcgccccgag cacgccatac accagcacgc ggcgttgtaa
    13741 tgccgccgga acggagaaat agctgaacaa catcagccag acaaagacgt tatcgaccgc
    13801 cagcgacttc tcaatcagat aacccgtgag gaaggccagc gcctgtggat cggcaacggc
    13861 gcgaccttcg gtttgtacca gataccacca gaaagcagcg ttaaacagta acgacagcgt
    13921 cacccagacc agcgaccagg ccgcagcctg tttcatggtc atggcatgtg ccccacgacg
    13981 tccctgcaac aacaggtcga tagccagcat aatggcgaca acaacagcga atccgcccca
    14041 tagcaacggc gtgccgacag tattcataga agttccttac acataaaaaa aacggccaac
    14101 gtcggaagac gtcagccgct gctttttatg catagacctc gccttccggc aaggtctcac
    14161 ttacaacaaa aaaggaatac gtctgcgtat tcctttcggg ttgcccggtg accggatgtg
    14221 gtttttcaca catcgtaatg acgatcgacc ggcaatgaag ttactcccct ttgcaggtaa
    14281 caaagtatga cagaccattc agtcagtcaa tggtctgtta catacctttt acactgtttt
    14341 actttggcaa ttatagtttt acgctatctg ccggaaaaat caccccagtc tggcggcgga
    14401 tctcggtcag cagtttggct gtgatgcgac tgaccgccag ccctggatga tccaccagat
    14461 gctcatccac cagggtagcg aacagctctg cttcgtagag catggtatta atatgctgcg
    14521 gctgggtgag atcctgcatc tggctgccac gcggcacgaa gcacactttc tggcattcag
    14581 acagtttttc aatcaccagc gatcccgctt cgccctgaat ctcgcttgcc aggacagaat
    14641 cgctgacctt tgagtgctgc aaggtgacgc tgaaatcacc gtaatccatc accaccacgc
    14701 cgtgggcatc tacaccactt gccagtaaac tggcggttgc ctgcacgctt ttcggctcac
    14761 caaataacgc taccgccgac gccaggcagt aaaagccgat atccataatt gaaccgttag
    14821 agaatgccgg attaaaggtg ttgggattct caccgtccag gtaacgttga taccgcgagg
    14881 aatactggca atagttgaaa aaaactttcc gcagtttgcc gactttcggc agcgcctggc
    14941 gtaacagatg aaagttaggc aggctggccg ttttaaatgc ctcaaacagc accacctgat
    15001 tttcacgcgc gcaggcaatg gctgcatcca cttccgccag attcgacgcc agtggtttct
    15061 cacaaatcac atgaattttg tggctcagga aaagttgcgt ctgggaaaaa tgcagggcgt
    15121 tcgggctggc aatatacacc gcgtcgatag cgtcactttc cgccattgct tccagcgaag
    15181 taaacagatg ctcgacagaa aactcattgg cgaagtgctg ggcctgttca aggctgcggg
    15241 aataaacggc ggttaactta tatttaccgc tttcatgtgc ggcctccacg aactggtgag
    15301 ttatccagtt cgtaccaatc acggcgaaac gtatcataac gcgtggatac tccataaagg
    15361 gattctgtcg tcaatgtagc acgccttttt catcttcgac gtgcgcggat acgcattatt
    15421 tctgcgccgg acaaccatgc tcactaaacg gaccaataat gaccgacggc ccgagatatt
    15481 ttggctgacg attgaagatt ttcgcgtcaa tcaccgcgct gactctggcg agtttctctc
    15541 gcagacggac aatctttccg cgtccttttt ccaggtctgg tgcgttaaac ccgatagcat
    15601 cgatgccgta ttgttttgcc agccagatgg cgcgttggtt atgaaattcc tgcgagataa
    15661 tggtgatatg gttctcccca aacactttat tggcacgcac cactgaatca agcgttgaga
    15721 atccggcata gtcacagaag atcacttttg ccggtacgcc ctttgcgatc aaagcctgct
    15781 gcatgcctga cgcttcatcg taatttttac gtccgttatc gccactgacc agcagccatt
    15841 tcacttttcc ggcgtggtac aactctgctg cggtatcaat acgtcgggta aagtagcgat
    15901 tacccggcct ggcccccagc aataagccca cgttacgcgc cggaacgacg ttgacgtcgc
    15961 tccaggtcag ctgtttgctg gcgttgacca ttacgcgatc ggcgataaaa atggtcgctc
    16021 ctgccacaag taacagtaag caggcaatct ttagagtccg gcgagaaaaa caaatacgga
    16081 gaagaaggcg ggcaaatgcg cgcagcatgg tcgttatcct taacgaaagg ggggaggtcg
    16141 attttaggcg ctaaagaaag aaaagataac gcctgacatc attttcagat tttgtcctgc
    16201 ccaagcgaca actgggttag ccacagtcgg gtgtcgaact caagctggtg gtactggggg
    16261 tccatatgac agcacaactg gtaaaacgct ttgttatgct ctttctcctt caggtgcgcc
    16321 agctcgtgca ccacgatcat gcgcaaaaac ggttccggcg cgttgcgaaa cacggtagcg
    16381 acgcggatct ctgcttttgc tttcagcttg ccgccctgca cacgagaaac agcggtatgt
    16441 aacccgagcg cgttttttag cacatggatc ttattgtcat acatcacttt attgatcggc
    16501 ggggcattac gcagaaactg atttttcaga tcctgagtat attgccagag ggctttatcg
    16561 gtggcgtagt cgtgtgttcc cggatagcgt tttgccagca catcgcccag acgctgttcg
    16621 ttaatcagtg ttcgcacctg ggaaagtagc tgctcgggat aaccctgaag ataagtaaga
    16681 ttgctcataa ccgcccacga aaaagagaaa aagggtatac tcacgcaccc ttttcagggg
    16741 caatgtcgaa attttatcat tcaggagggc cgatgagcca cttagacaac ggtttccgtt
    16801 cactgacact acaacgtttt ccggcgacgg atgacgttaa cccgctacag gcgtgggaag
    16861 cggcggatga atatttgctg caacagttgg acgacacaga aatccgcggc ccggtgttga
    16921 tcctgaatga tgcctttggt gcgttaagct gcgcgctggc ggaacataag ccgtacagca
    16981 ttggcgactc atacatcagt gaactggcga cgcgcgagaa tttacgcctc aacgggattg
    17041 atgagtcgag cgtgaagttt ctcgacagca ccgccgacta cccgcaacag ccgggtgtgg
    17101 tgctgatcaa agtgccgaaa acactggcat tgctggaaca gcaactgcgt gcgctgcgca
    17161 aagtggtcac gccacaaaca cgtattattg ccggtgccaa agcccgtgac atccacactt
    17221 ccacgctgga actattcgaa aaagtgctcg gcccgaccac caccacgctg gcatggaaga
    17281 aagcgcgcct gattaactgc actttcaatg aaccaccgct ggccgatgcg ccgcagaccg
    17341 ttagctggaa gctggaaggt actgactgga ctatccacaa ccatgcgaat gtcttctccc
    17401 gcaccgggct ggatatcggc gcgcgcttct ttatgcaaca tctgccagag aatctcgaag
    17461 gggagattgt cgatctcggt tgcggtaatg gcgttattgg tctgacgctg cttgataaaa
    17521 acccgcaggc gaaagtggtg tttgtcgatg aatcgccgat ggcggttgct tccagccgtt
    17581 tgaacgttga aaccaacatg ccagaggcgt tggatcgctg cgaatttatg atcaacaacg
    17641 cgctctccgg cgtggagcct ttccgcttta atgctgtgct ctgcaacccg ccgtttcatc
    17701 agcaacatgc gctgaccgat aacgtcgcct gggagatgtt ccaccacgcc cgccgctgcc
    17761 tgaaaatcaa cggcgagctg tatatcgttg ccaaccgtca cctggattac ttccataaac
    17821 tgaagaaaat tttcggcaac tgcaccacca tcgccacgaa taataaattt gtggtgctga
    17881 aagcagtgaa gctggggcgt cgtcggtaag attgacagct acctgccaga taaggcgtaa
    17941 acgccttatc tggcatacgt ttagcacatt acccccaggt cggataagat gcggcaagcg
    18001 tcgcatccgg caatgtctgc ccagttctgg aatcgcgctc cataaaacaa aaaatccctg
    18061 tcaccatcct ctacattctc ttgtttagcg ttgtttaacg tttattcttc cgtcacacag
    18121 ataaattcat ccgttgccaa tctgtcaacg gatgttatca tattgccaat ttgtcaacgg
    18181 acgtgatgaa gaatgcacct gataactcaa aaagcattga aagatgctgc ggaaaaatac
    18241 ccgcaacata aaacggagtt ggtggctcta gggaacacga ttgctaaggg atatttcaaa
    18301 aaacctgagt cattaaaagc agtattccca tctctggata acttcaaata tctggataag
    18361 cattatgttt tcaatgttgg gggcaatgaa ttacgtgttg tagcaatggt cttttttgaa
    18421 tcgcaaaagt gctacatacg tgaagttatg acgcataaag aatacgattt ttttaccgct
    18481 gttcatcgta ctaaggggaa aaaatgattg cgattgccga catcttgcat gcaggagaaa
    18541 agctaactgc tgtggcacct tttctggcgg gtattcagaa cgaggaacaa tacatccagg
    18601 cgctggaact ggtagatcat ctgctgctca acgatcctga aaacccctta ctggatctgg
    18661 tgtgtgccaa aataaccgcg tgggaagaat cagcgcccga atttgcggaa tttaatgcca
    18721 tggctcaagc catgcctggc ggtatagccg tgattcgtac ccttatggat caatatggtt
    18781 taaccctttc cgatctgccg gaaattggca gtaaatctat ggtgtcacgc gttttgagcg
    18841 ggaagaggaa attaacgctg gaacacgcta aaaaattggc aacgcgattc ggcatttctc
    18901 ccgccttgtt tattgattaa cgcgacgtgc cggatgcgtt gcatccggca cttcaatgca
    18961 cgatttaaat ctccagcgcc agccgtgttc cctgggcaat tgcccgtcgt gcgtccagct
    19021 ccatagccac atcgcagccg ccaattaaat gcaccgtttt cccgctatca atcagcggct
    19081 gcgccaatgc gcggtttggc tcttgccctg cgcagatcac cacattgtcc actgctaata
    19141 cctgcgtttc gccgttgatc accacatgca gcccgtcatc gtcaatcttc tgataactta
    19201 cgcctgggat cattttcaca ccccgcgaga gcagggtggt gcgatggatc cagccggtgg
    19261 ttttgcctaa cccctgtccc ggtttgctgg ctttgcgctg gagcatcaca atctgccgtg
    19321 ggctacgcgg gatctgcatt ccctgcgggc ttaagccacc agcctgttgt aggctgctgt
    19381 cgatccccca ttcattacag aacccggcga tattctggct ggtggattcg cccggctgac
    19441 ttaaatacat cgccgtatca aaaccaatcc cgccacaacc gatgatggca actttgttgc
    19501 ccaccggcgc tttgtcgcgc agtacatcga gataactcaa taccttcgga tgatcgatcc
    19561 cgtcgatggg cggagtgcgc ggcacgatcc cactggcgag gatcgtttca tcgaacgcct
    19621 gtaactgatc cgccgtcacg gtgtgattga gttttagcgt cacgcccgtc acttcgatca
    19681 tccggcggta atagcgcagc gtttcgtaaa actcctcttt gccagggatc tgtttggcga
    19741 tattaaactg cccgccgatc tcactgtgcg cgtcaaacaa cgttacctga tgaccacgcg
    19801 ccgccgcgtt aatggcaaac gccagcccag caggtcccgc accgaccacc gccagatttt
    19861 ttttctgcat ggcgggaagg atcggcattt tggtttcatg gcaggcgcga ggattcacca
    19921 gacacgaggt gaccttgcca acgaagatct gatcgagaca cgcctggttg cagccaatgc
    19981 aggtgttgat ctcatcggct cgccctgatt gcgcttttga cagcagttcc gcatccgcaa
    20041 gaaacggtcg cgccatcgat accatatcgg catcgccgcg cgagagaata tcgtcggcaa
    20101 cctgcggatc gttaatccgg ttggtggtca ccagcggcag cgagacgtga cctttcagtt
    20161 tgcgcgtgac ccagctaaat gcgccgcgtg gcacgggcgt ggcgatggtc ggaatacgcg
    20221 cttcatgcca gccaatgccg gtgttgataa tggtcgcgcc cgccgcttca atggcctgcg
    20281 ccagctctac cgtttcggca aaagtcccgc cgccttcgac caggtcgagc atcgacagtc
    20341 ggtagataat aatgaagtcg ttgccaacgc gttcgcgtac cgcactcacc acttcaacgg
    20401 caaaccgcat ccggttgtgg taatcgccgc cccactggtc actgcgctga ttggtgcgca
    20461 gcgtcagaaa ttcgttgatc aaataccctt cggaacccat tacctctaca ccgtcgtatc
    20521 ctgcctcccg tgccagttgc gcgcagcggg cgaaatcgtc gatcagttgc aggatctctt
    20581 catgggtgag ttcatgagga acgaaacggt tgatgggggc ctgcaatgcg gacggtgcga
    20641 ccagatgcgg ttggtagctg tagcgcccgg tatgcaaaat ttgcagggct attttgccgc
    20701 cttcctgatg taccgcttcg gtaatggtgc gatggtgtgg gatctggctg gcgtcattga
    20761 gcattgcgcc gccttccatg ccaacgcctg ttaaatctgg tgcaatgccg ccgctaacaa
    20821 tcagcgccac gccgtgacgg gcgcgttcgg cataaaacgc tgccagccgt tcagcaccgt
    20881 ccgggtattc ctccagcccg gtgtgcattg aacccatcaa cacgcggttt tttaacgtgg
    20941 taaaacctaa atccagcggg gcgaacagcg acgggtagct cataaaattg tccagtatgt
    21001 aaaataattg ttatgtggtc ggatgagttc taatttagcc gtcgccggag aaaagggaaa
    21061 aggggagtgc ggggtttgtg atgggattca aaaagaatac gcccggtggt aacgccggat
    21121 gcgacgcttg ccgggcccta tccgacctgg gggattgcac atgtagggcg gataaggcgt
    21181 tcacgccgca tccgccagtg gcgcggtgca gacgccggat gcgacgcttg tcgggtctta
    21241 tccagcctac gggaacgaac atgtagggcg gataaggcgt tcacgccgca tccgccagtg
    21301 gcgaggtgca gatgccggat gcgacgcttg tcgcgtctta tccagcctac gggaacgaac
    21361 atgtagggcg gataaggcgt tcacgcagca tccgccggtg gcgaggtgca gatgccggat
    21421 gcgacgcttg tcgcgtctta tccagcctac gggaacgaat atgtagggcg gataaggcgt
    21481 tcacgccgca tccgccagcc gttgcaaatt attgcttacg gaaaaaatcg ttatacaaca
    21541 tataaaaatg tgcggcactc caggagaaat ttggtgcgcc ttgctgtgcg cctgtcagcg
    21601 ggttgtaatt ttcctgaatt gggccatcgg cggttaaccc tttggcgtgc cggaagaacg
    21661 tatccgccag tttcagggca tcatcgcgat aaccgtaacg ctccatccct ttcagaccaa
    21721 accagaactg atccacccat acgcgcccgc gccagtagat atcagcgcca aaggccggat
    21781 tggttaacgc cgccgttccc agcggaacaa aggtattgaa ctctttagga tcgagcatca
    21841 ccttcaccac cgcgtcggca ttggcctgcg ttgccgcacc gttaaacagc ggcgaccagc
    21901 cttccggccc tttaccgcgc tcaacaatcg gtttgcccgc gcagccgttc gccagcggtt
    21961 tatcttcaat acgcacgtca tagaagtact gcgtagccgg gtcgaacata caggtgttga
    22021 tgtagtccgc gagctgctgt gccaactggc gatagcgctt agcctcttcc ggtttaccga
    22081 gaatcgtcgc catctccgcc agataatggt tatcgctgta catatagctg gcctgatcca
    22141 ccgactcctg caatagcgag taccccagca acgttccgtc ctgactgcgg ttttcggcga
    22201 atttcactgt ccagtcgcta cgtttgccgc cgcttgcgac atatttatcc agctgttctt
    22261 tgtcgataaa cccaaagacg gcggcatcat cacgccccga ttcccacgat gcagcaacct
    22321 gtgccggaat ttccagactg tcgtactggc ctttctccac cacgcgggcg tagttgttca
    22381 gcccagactg cgtctcttct ttgttgcctt tcttcaccgt aaacagcatc tcgccgctct
    22441 cagtgttgtg ggctttgtcg cgggtcgcgc catattccgg cacgccgttg ccgttgtgat
    22501 cgcggttacg taaccaccag tcgtgatagg ccaccagttt cgggtacatc tctgccaccc
    22561 aggttttatc ctgggtgacg ttgtacactt ccatcaccga ccaggcggca aggctgggtt
    22621 tggtatttcg ttcgttccag ttgccaccat cgccaccacg ctcggggcta agattccacg
    22681 ctatcaggtc ggggacaaag cccacatcct gcggacgcac gctgtcgcca ggctggatct
    22741 gccaggagaa gaccgcgcgg atattctctt tggcgatgtc cggattgaaa tgcgccatcg
    22801 caaacgcctg cttccaggta tcccacggcc aggtctgatt gccggagaac cagcgcccgg
    22861 tcaccgacgg cgtgacggtg ttatatttca ccgcaccccc aggcgagcgc cagttaccgt
    22921 tgagcgtttc gatcgctttc accgcgacgc gtgtctgttc cggcgtcgca tccggattgg
    22981 ttaacccttt cttcagatac tcttcccagc gttgctgcga ggcggtgaga taaaacgccg
    23041 gacgcgccag aatatcgcgg atctgcattt gctctttgct aacttcctga gaggtcagca
    23101 gatgggaata ggtggtatag agcgtcgtcg aaccgttgat atgcgcctta ctggtaaagc
    23161 gattgccgtt gatttcagtc tgcaccggca gggatttatg cacctgatat tctgattcac
    23221 cggaggtcag cagatcccag gtggcgcgca ctttgccaaa ggtgactttc aggccatcac
    23281 gggtggcgct gattttgcgc tggtagtcag ggtattcgcc agcaatggtt ttatcggaaa
    23341 gcggtttccc ttctttcgct tccagttttt ccagcagttc gccatcccac accagatcca
    23401 gcggtttatt gctgatgatt ttagtttcca gtagtgacgt gcgcggcgtg gcgaagcgca
    23461 gagtcatttc gacctgcaca tcttttgctg tcagtttttg caccagcgcg ccgggaatat
    23521 tgtatgcctc cagcgtgaag ttgactttct tgccgtcctg ccagacggtc aggcggtcga
    23581 aattgctggc cataaagttg atgtattctt ccgtcagcag cgcaacgccc ggaaagccgc
    23641 ccatggtgtt cgggccgtct ggcaacagat gaccatgcca ggctccgaga tcgaaaaacg
    23701 gattaaaacg ctggtgatcg tcgtaatcgt aatccttcat gtactgcggc gcaccagtac
    23761 ggttaatcac gtttttataa ttgtcggcgt tagtggcatg ggcggaaaaa cttatcagca
    23821 gagcgcaggt tactggcgtt aaaatagttt tgattttcat gaaattacag tccttattac
    23881 cagtagaaat attgcatcag gaagatttgg tcgctgggat cgttagaggc atttttcatg
    23941 tagaagcggc tatcaaaagc cgttgcgaaa cgtccgccgc catattcgta ccagatacca
    24001 aactgcatga aagaggtggt ttcatcgtca gcgtggtcag ggcgatgttt ggcgtaggtg
    24061 taagcggtgg agagataaac gccctttaac gcttcataca tggcgctcac catcatcccg
    24121 ctttcacttc ccggtacgtc cagcccgtcg gcacgtccgg tatggtgcca ggcgcgagcg
    24181 gcaaagttag gggtaagcag tcccgtgaga tagagttgtc ctgtgccgtc ggtaatttcc
    24241 agaccattca tccaggtgat gccttcggca aggtcatact ggatgtaacc gttcatcatt
    24301 gccggatagg tgtatttgtc gtcgtagcga tcgtacttgc caaaatgtag ccatgctttg
    24361 ctttcgtcat gatgtccggc aggtgtggcg gtgacgctat aacgaaagtt gccgcttaag
    24421 ttctgcacct tcagcgcggt cataatgtcg cgggtattgg gaatgacgta gcccagatcg
    24481 ggtgtgaagt cgccccacca ttgcaggtca tccagcgagg aatcctgacg cagactgagc
    24541 atgatttcgg tgccatcatt ggttcgataa ccgccataga aacggttaat cccaccttcg
    24601 aaaccgcccc agctatggtc aggcacccag gcattgccgt cgtggtcggc ctgcacggtc
    24661 cagccttcgc cgtaaatcag gccgaaccag gagccgtact tcatctccag gccaccttca
    24721 atataggtgc cgtcgctgta tttatgttta ttctcaccct ctaaatccat atatccacca
    24781 acccctaatt cgccgtaaaa acgaaatgaa ggagaagatg tcgaggtaat tgcgggggtt
    24841 tttgtgtcgt catgttctgc cggacgtgct tcttctcctg ccgcagaaaa ggcgtagcaa
    24901 aacggcagag caagtaatgc tctgcatggt gcagtgatta atttcattgt gttaatccat
    24961 gagctaaaga aaaagtacag ccttttatcg ttgtaattat aaaaatataa aggcagaaac
    25021 tattttccct ctcctcatcc aggaagaggg aaaatgcctg ctcatttttt attagtctgt
    25081 ttaatcaaca ttgctggctg gcgtcgcttc aatatggcgc tcttcagccg ttaatccctt
    25141 tatatattta ctgtatttcc accacgcaaa gccaaggaat ataacaatac cgccgacgtt
    25201 ataaaaaatg atggtcagaa tattcgcgcc agtcgggaat gtcgaagcga caaaccctac
    25261 ggcaaatatc gcaatcagca ttgaaacaac gataataccg gtgcggcggg agcccatacg
    25321 gaaatcgcgt ggcaggtgat ctaatttggc gcgtaaattc aggtaagcca gcatgatgaa
    25381 taacggcgga agcatggacg ctgcggcggt catattaata atagtattca tcagatcctg
    25441 cacagtattg gaaccgagca tcgggataat catcagcggg atgacgatca ggaactggat
    25501 ccacgctgcg cgcgccggaa cgccgttttc gttaagttcg acggttttct taccaaagat
    25561 gccttccggg atttcggaga agaaaatttt caccggcgtt gcggtccaca tcagcaggga
    25621 accgaacatt gcggtaaagg acaccagacc gacaaagcga ttcatcaacg cttccggcag
    25681 accaaaatac gccgccatgc cgtggaatac ctgcaccgat ccgccagtaa atttcaactc
    25741 tttgctgctg acgaagacgt taatcagcac cgaggagacg gaatatagta caccgataaa
    25801 aatcccggcg aggatgatca ctttaacgaa cgatttcgaa ccgcctttga cgtcgttaac
    25861 gtacacagcg acggactccg cgccacctgc ggccataaag atccaggtgg taacgccgag
    25921 gaacgcccag ttgaagttcg ggatcatcgc gtcaacggtg atggggtcag caggctgtac
    25981 gccgccaacc agcgccgtac ctgccagtaa aatgtaggag agcgttaaca gcagcatcag
    26041 cgttgaagtg acggaggtaa ttggccccaa cattttcgcc ccgttggtgg aaacccaggt
    26101 ggagaaggcg aacagcacca tactgataat ggtggtggca accggcgtca taatatattc
    26161 atagccgagg aaggcatacg aagcataagc aataacgcgc ggcaacagcg aggtgaaaaa
    26221 gaacaggttt acgaaccagt aggtataggc agtaataaat gcccaacgtc cgcccagcga
    26281 actttttacc cacgcgtaga caccggcttc tgagttttta tttaacgaaa caaattctgc
    26341 gatgatcaga cagaagggaa taaaataaaa aatcgtcgcg aggaaaaaca tcggtgccga
    26401 ggccagtcca agctcaatat tattattgat aacgttgtta aagctgtaaa cggcggcaaa
    26461 agtcagcgag agcaagccga atttgccgat tgtattacgt ttggtatcag acataacaca
    26521 tctccgaaaa tgagtagggt agcggtggcg gcaaactcct tttgccgccg tagttgttat
    26581 ttgttatgga aataaccatc ttcgatggtg actttgagaa ctactttttt gaccgcgtta
    26641 ttgcagataa accgatatgc ttcatggata tcgcaaataa cgatttgccc ttcgtcgacc
    26701 tcaacggttt ctccgcagcc ttttaaatat tcacggtcag tttcatcacg ataatattcc
    26761 acgacctgta atgtctcttt cggcgcatat tcaatttttt gctgcccttg caggtagtaa
    26821 tgcacttcaa aatagcgtcg atgcccggta aatagcgcat cggtcgcgga gtctgtctcc
    26881 acgcggtaag tcaatgagtc accgatggag tgggcgacgc caggctgaat gttgtcgata
    26941 ttttcaatcg cttcaacgca gcgttgccac ttcttaccag aggcgtaaat ctggcggaac
    27001 tgttctaagt tatcgatgat cctcattgct tattctcgct gtgcaaattt gtggaaaaga
    27061 accctgcgcc gaactcatac gacgccaggc tttgcgcggt agcttctccg ccagaaaccg
    27121 gcagcaacgt aaagccgtag ctgaagtcac ggaaccagac gcgccaggag tccagcacct
    27181 cgctgcccca ggaattggag ccgaggccaa gcagctggtg gtcgagattc agggtgatgt
    27241 catcactgcg ctgcagctcg ttacagtgct gggcagcgtg gatgttttcc tgggtatagt
    27301 gccaggcgct gaagttaatt gggcgctgcg gaaccaccag cagaccgttg ccgtggcggt
    27361 tagtcagtgc cgtccagcgg acatgctgac ggttgccgtt gttctgcggg aagggatagt
    27421 tctcgaatat ggcatcgacg gtgctgcgcc agatatcgat gatgttagcc tgctggctgt
    27481 cggcgtagtt ttcgcccggt ccacgaccgt aatacgccac ctgatcgtat tcgccgttaa
    27541 tccccatggt gaaaccgatg cacggaatga tgtgcggata gtcgccgtaa cgctcgccgg
    27601 aaagcgccac gttaacctgg ccatctgcag cgatgcgcca gatgtaggta cagcgcatcc
    27661 cgaagtcaaa caccggcggt gctataaccg tgcggctgat gatcaacact tcaccatcgc
    27721 tctgttctac ggcaaagtcg cgcagatgtt cctgcatgat ctgcaaatga ttcggctgcc
    27781 acaacccttc gtactcctgc ttgtggttgt cgatcattgg cttgaagaag ttgatctttg
    27841 gctcgcgggt cagcagcgat tcgccattta cctgccagga tgtcggttta ccactcgttt
    27901 ttgagaaggt gatcgcaaag ttgtagccgc gaacagtaca gctcaaacga tcgtcttcca
    27961 gcgtcagcgg acgggcgtta ttcggtgcga aaggtacagg ctgcgcggtg ttttccttca
    28021 gcgggaactg ataagtggcg atagaatgcc cggcttcgct gtagcgggtg cgggaatctt
    28081 tggtcaccgt aatgttgagg aacgcttcgc gggcgtccag ctgcggcagc gtgatctgca
    28141 agggggcttc gctgttcggc gcaacgtcgc gcagtttaat ctgttgcgtc gcgagcgttt
    28201 caccttcggc gcgcacctct gcgtgcaggg tgtagtcatc cagcgtggta aaccacagtt
    28261 tattttcgac tttcagctcg ccgcgagtca gatccagcgc gtggattttt accggcgcga
    28321 taacctgttt gtactctttc aggcctggtc ccggcgtctg atcggaatag atcaaaccat
    28381 caagacagaa gttatagttg ttgggatagt cgccgtagtc gccgccgaat ttataccaga
    28441 cattgccgtt gtcatcctgc gcctggatcc cgtggtcgca ccactcccaa acataatgtc
    28501 cctgaataca atcgtgctta tagaagacgt tctggtactc cgtcagcccg cccggtccgt
    28561 tccccatcgc atgagcatat tcacagatga tgcgcggctt cggatgcggg tattcaccaa
    28621 actcattcat cagcggcacg cgggtgtaca tggtggaaat aatatcgacc acttcagcat
    28681 cgcgatcttc ttcgtaatgc accagtcgcg tgtcatccag cgccttcgct gcgtggtaca
    28741 tcgcgcggat gttacagcca tagccggatt cattgcccag cgaccagatg atgatcgacg
    28801 gatggttttt ctgcgcgtga atatggcgaa caatgcgctc gacgtagact ttttcccact
    28861 gcggatcgtc ggtaatacgg ctgatatcgc cgacattagc aaagccgtgc gattcgacgt
    28921 cggtttccgc catcacaaac aggccgtaga tatcacacag ttcgtaaaaa cgcggatcgt
    28981 tcgggtagtg agcggtacgc acggagttga tgttgtgctg cttcatcaac tggagatctt
    29041 tctcgacgcg atccattcca acggcgcggc ctttgcgatg atcgttgtcg tgacggttga
    29101 cgccgtgcag catcacataa cggttattga tccagaacag accgtcgcgc actttgatat
    29161 cacggaagcc aacgcgttgt ggcaccactt ccagaacgtt gccgttggcg tctttcagcg
    29221 tcatgaccag atggtaaaga taaggggatt ctgctgacca ttgctgcggc tgttcgacag
    29281 taaaagcaaa gctggcgctg gtcagttttt caattgccaa atgatcaatg gcgctgctgt
    29341 gcaccacgcg ttcgccatca aacagggtat attccagcgt cgtgacgaca ggggaggcgg
    29401 cgagattttc cagcaccact tcgcaggaaa gcgtggcatc gcaataggct tcgtcaaagt
    29461 cggtacgcac agtgaaatcg ttaatatgcg ttaggtgttt tccgaccaga taaacatcgc
    29521 ggaagatccc cgctgaccac cacatatcct ggtcttccac gtaggtagag tccgcccact
    29581 gcatcacgcg cacacacaac aggttgtcgc cggttttaac catcgcgctg atgtcaaact
    29641 ctgcggtcag gcgactgccc ttgctgaaac ccacatactg accgttaacg tagacttcaa
    29701 aataggtttc gacgccgtca aatttaatca gcgtctgttt accctgccag ccgtcactga
    29761 gggtgaaaat acgttgatag gcaccggttg ggttatcgct gggaacaaac ggcacatcga
    29821 tggggaacgg aaaaccttcg tcggtatatt gcagtttgcc gtgaccttcc atttgccaca
    29881 tggcggggac ggtaatatgc ccccagtcag ccattaactc agaggtgaag gcttctggca
    29941 cttgcagcgg atggtcaaaa aagtggaaat tccactgacc gcttaagggc agaaacaggc
    30001 tgctcgtttc gcgggcaaag gtacgcgctt gcgcaacaga atcatatgaa aaaaagtacg
    30061 cacgcggcgc aagtcggttt tcgtgggtga gctgaatgtt ttcccagcga ttcatagggc
    30121 ctcccggtag agagaaatcg gaaccattca tggtgatggt agtttgtgta attgctgggg
    30181 agtttagtaa aatttttact aaaaagtagc gggtaaatgc atttacttta actggatcac
    30241 gaaaataaac ggcagcactg cccggcaaaa acgattgccg gacagtgtgt aaggggattt
    30301 aacgggtcgt gccgcgcagt tttaatttgc tgggaacgaa gactaacagc ggcagcgcgc
    30361 gaccatcgcg ggctttttca tacaccaggt ttacgccctg acttcccatc atttcggaat
    30421 ggatgcgcac ggtggagagc ggcggaaagg taaatcgcgc ggtggggata tcgttaacgc
    30481 tgataagcga aatatcctgt gggatgttca gaccacgttc atgaatcgcc cgcagtacgc
    30541 cgatagcaat ggaatcggaa gcaacaaaca gtgccttcgg atagtcttcc cgcgccagca
    30601 tttgttttgc cagttcataa cccgacgaac tggaaaaacc gccgcgccag atatcctctt
    30661 cgcgtaccac ttgtttcagt cggccatatt ccgcaaaggc gacctcacga atatccgcct
    30721 tgccaggctc atcttcaccg ccaataaaac caatacgatt aacgccctgg ttgatataga
    30781 agtcgatgat ttctttactg atgcgtgcca gatcgatatc caccgcatcg taaccgctgc
    30841 cgggttcgtg aaagtcgata aaacagatat tgtcggtcaa cgcgctggca gcggcgcgca
    30901 gggcgggcgt gggtttgccg acaattaaaa taccggtgac gtttttaatg tctggtaatc
    30961 cgctgtgttc ataacagttg gtcagttcga tcgccagctt ttcgcactga gtttcaatgc
    31021 cgtggcggat cgccagatag taaggatcgt tgatctccag ctcctgctgg tagctgtaga
    31081 tagccagaat atggtgttgg ttgactgcgc ctgtctgtag tttacgggcg ctactggtct
    31141 tgtactccag cttttcggcg atctcgagaa tgcgatgttt cgtctcttct ttcacattca
    31201 atgtcggatc gtcatttaag accctggata ctgtcgccag ggatacgcca gcttcgattg
    31261 cgatgtcttt tagtgttgcc atgtttacct tttttacttt ccctgatgtg cgcccccatt
    31321 gtagaggaag tgcttatcgg gtcacctgtt ttcagtaaat aaagcgcgtt tctttgacgc
    31381 tgtaacggta acacagcggc tcacgaatgt gatcacgcct gcattctcca tttttagcct
    31441 acaggattga gaaaaacttt tgctatagtt tagtaaaatt ttactcgaag ggaggaagca
    31501 atggaaaccg tggcttacgc tgattttgca cgtctggaaa tgcgcgtcgg aaagattgtg
    31561 gaagtgaaac gccatgaaaa cgccgacaag ctgtacatcg tacaggttga tgtgggggaa
    31621 aaaacactgc aaaccgtgac cagcctggtg ccgtactaca gcgaagaaga gttgatggga
    31681 aaaacggtgg tggtcttatg caatctgcaa aaagcgaaaa tgcgtggtga aacgtcggaa
    31741 tgcatgctgt tgtgtgcgga aaccgatgac ggcagtgaaa gcgtgctgtt aacgccggag
    31801 cggatgatgc ctgcgggcgt acgtatcgta taaaaagatc ggatggcgac gtcgtattgc
    31861 catccgattt gatactacgc ttcttcgaca cttactcgca tggccgccag cgccttacgc
    31921 gccgctttga tcacgagttc acactgttca atggtcagtg tcagtggcgg ttcaatgcgg
    31981 atcgttttgg cgttattgag cgttccggcc accagtacgc gctggcggaa catctcgctg
    32041 gcaaagttat agccgatttc gttatcaaca aactcaatcg ccattaacat ccctttacca
    32101 cgcgcttcct gtaccagatc gggatattcc cgcgccagtt gacggaaacc gtccagcaac
    32161 atatcgcctt tttgctcagc ctgagccggt aagttctgct ccagcaacac attgatggtc
    32221 gccagcgccg ccgcacaggc cagcgggttg ccgccaaagg tggtggtatg caggaacggg
    32281 ttgtcgaaca gaactgagaa cacctcttca gtggcgatgg ttgcgccaat cggcatcaca
    32341 ccgccgccga gggcttttgc caggcacagg atgtccggct gtacgttctc atgttcgcag
    32401 gcgaacatct tgcccgtgcg ccccatgccc gtttgtactt catcgaggat catcagtgcg
    32461 ccgaactcat cgcacagctt acgcacggcg gtgagatagc ccggcggcgg cagaattacg
    32521 ccaccttcgc cctgaatcgg ttcgaggatc accgcggcca catcatcacc ggtttttttg
    32581 cactcattaa gagccgtgcg catggcttcg atattgccaa acggcacatg acggaaccct
    32641 ggcagcagtg gcataaacgg tttgcggaag gtcgatttcg ccgtggccga cagcgcgcca
    32701 agtgatttac cgtggaacgc gccgctggtg gcaataaaag taaatttgcc gcgcggcgac
    32761 tgataagcct tcgccagctt cagcgccgct tcaacggact cggtgccgct attacagaag
    32821 aagctgtatt tcagtttacc tggcgttagc gcagcaaggg ttttcgccag catagcccgt
    32881 aacggatcga gtagctcctg gctgtgcagt ggttgtttcg caagttgatt ctgtacggcg
    32941 gaaaccacaa ctggattacg gtgccccacg ttgaaaattc caaaacctcc caggcagtcg
    33001 ataaactcct gtccctgggt gtcgacaagc gtatttaaac ctcccgcttg ccactctacg
    33061 gctccgtaat ccccgccggc ggtaacagat ttgcgatact ctaaaaaccc cggattgaca
    33121 tgctctttga agtattcaat cacctctcgg ttaagtgctt tcatctcctc atgatccagc
    33181 gttcgctttt caatgagatt cagggcgtgg gcgctgcacg ctaaagccga tgcgctcgaa
    33241 ggtaacctgt tcaaaatatg ctccggaggc tcgcgtatca catgataccg atttaagtat
    33301 tgcagggatt gcgccactcc ggctgcgatc gcgaaaatcg ggataaacac aaatgtggtt
    33361 atcatttgcg caatatttaa tgctatttgt taacatctgt tgttattttg cacggttttt
    33421 gtcgggtatg gatttgataa ttgctgcgca aatgcacgct ttcagggcat taactgcctg
    33481 atttttgggc aagataatta acgttttcgt atagtaacag agtgaaagta ttttaaatca
    33541 acgaattaga tttgtgcttt aatcgcaaat tgcgatctaa atcaaattaa tcggttaaag
    33601 ataaccgcag cggggccgac ataaactctg acaagaagtt aacaaccata taacctgcac
    33661 aggacgcgaa catgtcttct catccgtatg tcacccagca aaataccccg ctggcggacg
    33721 ataccactct gatgtccact accgatctgc aaagctatat cactcatgct aatgacactt
    33781 ttgtgcaggt gagcggcttt accttgcaag agttacaagg gcagccgcac aacatggtgc
    33841 gtcacccgga tatgccaaaa gcggcgtttg cggatatgtg gttcaccctg aaaaaagggg
    33901 agccctggag cggcatcgtg aaaaatcgcc gcaaaaatgg tgaccattat tgggtgcggg
    33961 ccaatgcggt accgatggtg cgcgagggaa aaatcagtgg ctatatgtcg attcgtaccc
    34021 gggcgacgga tgaagagatc gcggcggtgg agccgctgta caaagcgctg aacgccggac
    34081 gtaccggtaa gcgtattcat aaaggcctgg tggtgcgtaa aggctggctg ggtaaactgc
    34141 cttcattacc gcttcgctgg cgggcgcgtg gagtgatgac cctgatgttt atcttgctgg
    34201 cggccatgct ttggtttgtt gctgccccgg tggtgacgta tatcctctgt gcgttagtgg
    34261 tattgttggc aagcgcctgt tttgaatggc agattgtccg cccgatagaa aatgtcgccc
    34321 gtcaggcact gaaggtggcg accggagagc gtaatagtgt tgagcatctg aatcgcagcg
    34381 atgagctggg gctgacatta cgcgcggtag ggcagcttgg cctgatgtgc cgttggttaa
    34441 ttaacgatgt ctcaagccag gtgtccagtg tcagaaacgg cagtgagacg ctggcgaaag
    34501 gcaccgatga actgaacgaa catacccagc agacagttga taacgttcag caaacggtgg
    34561 cgaccatgaa ccaaatggcg gcgtcggtga aacagaactc tgccacggcg tcggctgccg
    34621 ataaactgtc aatcaccgcc agtaatgcgg cagtgcaggg cggggaggcg atgaccacgg
    34681 tgatcaagac aatggacgat atcgccgaca gtacccagcg cattggcacc attacttcgc
    34741 tgattaacga tattgcgttt cagaccaata ttctggccct gaatgcggcg gtggaagcgg
    34801 cgcgtgccgg cgaacagggc aaaggttttg cggtggtggc gggggaagtg cgtcatttag
    34861 ccagccgcag cgccaatgct gccaacgata ttcgcaagct gattgatgcc agtgctgata
    34921 aggtgcaatc cggttcgcag caggtacacg ccgccggacg tacgatggaa gatattgtgg
    34981 cacaggtgaa aaacgtcacc cagttgattg cccagattag ccattcaacg ctggaacagg
    35041 ccgatggtct ttccagcctg acccgtgcag tggatgagct taacctcatc acccagaaaa
    35101 atgccgagct ggtggaagag agtgcgcagg tgtcggcgat ggtgaaacac cgcgccagcc
    35161 gactggaaga cgcggtgacg gtgctgcatt aatcggtatg ccggatccgg tatttgatac
    35221 acgatgcctg atgcgatgct ggcgcatctt atcaggccta cggggtgtgt agcgggtgta
    35281 ggccggataa ggtgcgtcaa gcgtcgcatc cggcaattgc accgcgccac tggcggatgc
    35341 ggcgattaat ccagttgcgt aatatcaaaa gcggcgcggt cgatcaccgc aatgatcttt
    35401 tttatttgtg catcagtgat atcactctgg ttgacgcgta aattcagcac cgctttaaaa
    35461 ttatctagcg cccgcttcat ttgcgggttc tggcgcagcg cagcgccaac gcaacgcgct
    35521 ttgatgcgtt cttcaatcat ctccacctgt tcgcggtttt cttccagcca ctgcgcgcct
    35581 tgttcggtca gcgcaatctg cttcttacca ccttcctctt cgcggatggt aatcagcgac
    35641 tgctcctgca gaaaatccag cgtcgggtag atgacgcccg ggcttggggt gtaattccct
    35701 tgggttagat tctcaatcgc tttaatcaat tcgtaaccgt ggctgtcatc gcgcgagaga
    35761 atatccagaa tcaccagacg caattcaccg tgaccaaaga aacgttgccg acgaccgccg
    35821 ccgcgaccgt ggcgaccacc gcaacattga ccgtgttcat gctggtgacc gtgtccgcag
    35881 tgctcgtgtt ctgacttctc acctttgcag cagccctcat ggcgtggctg gccttcatgt
    35941 ttacaacacc cttcgtgatg atggctcata tcagcctcct tttgcttaat tagatatatc
    36001 taatttatat ctgatgtgag cactcttgca agaataaaaa tgatttttaa atgtggactc
    36061 atctcacacg taggccggat aaggcgttca cgccgcatcc ggcgttgagt aatgatgcct
    36121 gagaagaggc ggttcgtctt gtcagactat agacatatca gttctacaaa tcgcttgcat
    36181 ttatcatgat taacaatcat tatcatttgc gaaaattatt tagatatatc tgattaccat
    36241 cacgaaggcg ataacaatga ataactcccc ccgctacccg cagcgcgttc gcaatgatct
    36301 gcgcttccgt gaattgactg tgctacgcgt tgagcgtatc agcgccggtt ttcagcgcat
    36361 tgtccttggc ggcgaggcgc tggacggttt tacatcgcgc ggctttgacg atcacagcaa
    36421 actcttcttt cctcaacctg acgctcactt tgtgccgcca acggtaacgg aagagggcat
    36481 cgtctggccg gaaggaccac gcccaccgtc gcgtgactat acgccgctgt atgacgaact
    36541 acgccatgaa ctggcgatcg atttctttat tcacgacggt ggggtcgcca gcggctgggc
    36601 gatgcaggcg caaccgggcg ataaacttac ggtggcaggt ccgcgcggtt cgctggtggt
    36661 gccggaagat tacgcgtatc agctgtatgt ctgcgatgaa tccggaatgc ccgcattgcg
    36721 ccgccgcctg gaaacgttga gcaaacttgc cgttaaaccg caagttagtg cgctggttag
    36781 cgtgcgggat aacgcctgtc aggattatct cgcgcacctt gatggtttta atatcgaatg
    36841 gctggcacat gatgagcagg cggtagatgc gcgtctggcg cagatgcaaa tccctgccga
    36901 tgattacttc atctggataa ccggcgaagg taaagtcgtt aagaatttaa gccgccgctt
    36961 tgaagcggaa cagtatgacc cacagcgggt acgtgcagcg gcctactggc acgcaaagta
    37021 actgatatat aaaaagaaaa aggctgacga tttctcgtca gcctttgcta tatctggtgg
    37081 cccctgctgg acttgaacca gcgaccaagc gattatgagt cgcctgctct aaccactgag
    37141 ctaaggggcc atggtagcgg attataaagt aactccgtgt cgcaatccag ccattaccgc
    37201 gcgcctgctg tttttataaa caatgtattt tcaatccgtt atacttttcc tggtgatgtt
    37261 agaaaggagt aaatatggtt gaggatattt tggctccagg gttacgggtc gtgttttgcg
    37321 gtatcaaccc tgggctttca tccgccggga ctggttttcc ctttgctcat ccggcaaatc
    37381 gcttctggaa ggtgatatat caggccgggt ttaccgacca tcagttgaag ccgcaggagg
    37441 cacagcatct gctggattat cgttgtggcg tcaccaaact ggtagacagg ccaacggtgc
    37501 aagccaatga aatttcaaag caagagctac acgcaggtgg gcgtaagctg attgaaaaaa
    37561 ttgaagatta tcagccgcaa gcgttggcga ttctgggcaa acaagcatat gaacagggat
    37621 tcagccagcg tggtgcacag tgggggaaac aaacgctcag cattggttcg acgcagattt
    37681 gggtgctgcc aaatcccagc ggtttaagtc gcgtttcact ggagaaactg gttgaagcgt
    37741 atcgcgagct ggaccaggcg ctggtggtgc gtgggcgata aaaaacgcca ccgaaaaggt
    37801 ggcgtttgtg caaagggaca ggtgccgggt gcggcgtaac gcctgatccg gcctaccgat
    37861 taatcgtcca ggaagctacg cagcacttca gaacggctcg ggtgacgcag tttgcgcagc
    37921 gccttcgctt cgatctgacg gatacgttca cgggtaacgt cgaactgttt acccacttct
    37981 tccagcgtgt gatcggtgtt catatcgata ccgaaacgca tacgcagaac tttcgcttca
    38041 cgcgcggtca ggccagccag cacgtcgtgt gttgccgcac gcaggctttc ggtggtcgca
    38101 gaatccagcg gcagctcgag ggtggtatcc tcgatgaaat cccccagatg cgaatcttca
    38161 tcatcaccga tcggcgtttc catggagatt ggctctttgg cgatcttcag cactttgcgg
    38221 atcttgtctt ccggcatcag catacgttca gccagttctt ccggcgtcgg ttcacggccc
    38281 atctcttgca gcatctggcg agaaatacgg ttgagtttgt tgatagtctc aatcatatgc
    38341 accggaatac ggatggtgcg cgcctgatcc gcgatagagc gggtgatcgc ctgacggatc
    38401 caccaggttg cgtaggtgga gaacttgtaa ccacggcggt attcgaattt atcaaccgct
    38461 ttcatcagac cgatgttgcc ttcctgaatc aggtcaagga actgcaagcc acggttggtg
    38521 tatttcttgg cgatagaaat aaccagacgt aagttcgctt caaccatctc tttcttcgca
    38581 cggcgggctt tcgcttcacc gatggacata cgacggttga tatctttaac ctgctcgatg
    38641 gtcaggccgg tttcttcttc aatctgctgc agtttctgca gggcgcgatg cacttcttca
    38701 gagacatcgt gcagtttttc cgaccacggc ttgttcatcg caattgccgc gttgaaccag
    38761 gtatcgctgg tttcgttgcc ggtaaacaga gtaatgaagt ttttcttcgg cattttgcac
    38821 tgctcaacgc agagcttcat gatcagacgt tcttgcgtac gaacgcggtc catcatgacg
    38881 cgcatactgt tgaccaggta gtcaaactgc ttcggcacca ggcggaactg tttgaatact
    38941 tcagacagtt tcaggatctc ttcctgagcg gcagcgtgac tgcgaccttt cgctttgatg
    39001 gtgtcacgcg ttacaacgta ctgagcgcgc agttccgcaa atttttcgcg agccagttcc
    39061 gggtcgatgc tgttgtcatc atcggcgctg tcgtcatcgc catcttcttc gtcttcatct
    39121 tcgtcatcgt ccagatcttc ctgggaaagc tcagaaccga cgtgagtggc ggtaggtgcc
    39181 agatcttctt ctgcgttcgg gtcaacaaag ccggtgatca gatcggacag acgcgcttct
    39241 tctgcttcaa cacgatcgta ctgttccagc agataggtga tcgcttccgg atattcagca
    39301 acggagcatt gaacctggtt gatcccgtct tcaatacgct tagcgatgtc aatttcgcct
    39361 tcgcgggtca acagttcaac ggtgcccatt tcacgcatgt acatgcgtac cgggtcagtc
    39421 gtgcgcccga tttcagattc cacgctggaa agcacctgcg cggcggcttc ggcagcatct
    39481 tcgtccgcgg tgttttcagc cagcatcaga tcatcggcat ccggtgcttc ttccatcacc
    39541 tgaatgccca tgtcgttgat catttggatg atgtcttcga tctgatctga atcgacgata
    39601 tcttccggca gatggtcatt gacctcggca taggtcagat agccttgctc cttaccacgg
    39661 gtgacaagaa gtttcagctg tgactgcggg ttttgctcca taagacggta tccacactta
    39721 tttcatgagg ttggtgttgg gcgattgacc cgatgtcctt cagccgttaa cgctgaaatc
    39781 caacggttgt ttaccgccaa cgataattac gagggcgtac ttatatattt gccgctgcct
    39841 ctcagtgcgg ctgtcggggg cttcccgatc gctcttcggc acttaagccg ttaaatcact
    39901 ttttcgccag ctcctggttt aatgtccaga gctccaggcg ttcttcgttg cttaaaccat
    39961 gcgtgcgctc acgagcgatt aactcttcct ggcgcagttc aagcagcgaa tcaaacatat
    40021 ggttgagtga gtcggtgaag gtttgctcag caatattctt atctgctata tcgtcccaca
    40081 tcgacagttt ttcaagggtg gcagcattat ttgtaccacg atagtgctct aaaagttgcc
    40141 cggtggtcag acctggctgg gagagacaag tgttgaccag ttctctgaat aagccaagtc
    40201 cagggagctt attttcatcc agattctcaa gcggcgggac caacgtcgct aattctggat
    40261 tttgcaccag caaccctata agtatacgca tggtcgtgcg ttttagctgc ggaacagggc
    40321 gagaaacgcc gctctctgcc gcttttggca ttaatcgttc aagctggctg tcatcaagta
    40381 tgcctaattt gttgcctaat tcctgacgaa gatatattcg cagcgtttcg cccggcactt
    40441 gcgatatcaa tggcagtgcc agcgtactca aacgtgcgcg cccgtcaggg gtactcagat
    40501 caacttgcgg catcaggctg ttaaacagaa atgcggaaag cggcatcgcc tgctccatcc
    40561 gcgcttcaaa cgcttcttta ccttcttttc gtaccagcgt gtcagggtct tcgccatcag
    40621 gcaaaaacat aaagcgtagc tgacggccgt ctgtcatgta aggcagcgcc gtttccagcg
    40681 ctcgccatgc ggcatcacgg cctgcacggt cgccgtcata acagcaaatg acattgttgg
    40741 tcgcgcggaa caacagttgt atgtgatcgg cggtggttga cgtacctaac gacgcaacgg
    40801 cgtaattaat gccgtattgc gccagcgcca ccacgtccat atagccttcg accacaagca
    40861 gacgattggg ttcagcgtta tcctgctgcg cttcataaag accgtaaagc tggcggcctt
    40921 tatggaaaat gtctgtttcc ggcgagttca ggtatttggg ggtatcgttg cccagcacac
    40981 gcccgccaaa accaatcacc cgaccgcgtt tatcgcgaat ggggaacatc acccgctcgc
    41041 ggaaacgatc gtaactgcgt ccctgatcgt tagtgaccaa catccccgca tcaatcaatg
    41101 actggcgatt ttctggattg ccgccaaacc gcttcaggac gttgtcccag ccgggaggcg
    41161 caaaaccaat cgcaaagcga gcgataacct cgtggcttaa tccgcgtttt tccagatact
    41221 ggcgcgcaga cgtggcaaca ggttgttgta aagattgttg gtaaaacgta ttcagaccgt
    41281 ccatcaactg ataaagcgtt tgccgctgat ggcgctcgat ctggctgggg ccgctacctg
    41341 cttcaaatgg cacttcaaga ttgtgcattg ccgccagctc ttcgaccgtt tcgacgaact
    41401 caagcttgtc gtagttcatc aggaagtcga tcgcgttgcc gtgcgcgcca catccaaagc
    41461 agtggtaaaa ctgtttctca ccgttaacgg tgaaggacgg ggttttctcg ttgtggaatg
    41521 gacaacacgc gtggaaattc ttgccctgct ttttcagctt cacacgggca tcgatcagat
    41581 cgacgatgtc agtgcgtgcc agcagatcat taatgaatac gcgtgggatt cgtccagcca
    41641 taggccccga tttttagcaa ttcataaacg aaaataagcc gcgcattcct ttcggaagca
    41701 cggccttaca actacaactc ggtctgatcg gagagcaacg ctctcgggga attagtacag
    41761 acgagtgcgg cgtgcgtttt cgcgagccag tttcttcgcg tgacgtttca ctgcagaagc
    41821 tttagcgcgc ttacgttcgg tagtcggttt ttcatagaac tcacgacgac gaacttccgc
    41881 cagaacacct gctttttcgc aggaacgctt gaagcgacgc agagctacgt cgaacggctc
    41941 gttttcacgt actttaatta ccggcatgtg cctctcacct ttgattaatt cggtttgccg
    42001 ctggcatcaa cgccagctta tttcaaaatg gtgcggaatt ttactgcaat tgctgctgct
    42061 ttgtaaagca ccgcggcctt ttttgaaagg gacttttata agggtgagga gtatacacga
    42121 ggctttctcc aggggcgaac aaagttttac atcaacccgc attggtccta cactgcgcgg
    42181 taataaagcg aggtaaaaca agtcatgcgt gtactgggta ttgaaacttc ctgcgatgaa
    42241 accggcatcg ccatttacga cgatgaaaaa ggtttgttag ccaaccaatt gtatagtcag
    42301 gtgaaattgc acgctgacta cgggggcgtt gtgcctgaac tggcctcccg cgatcatgtg
    42361 cgtaaaaccg taccgttgat ccaggcggcg ctaaaggagt ctggtttaac ggcaaaagac
    42421 attgatgctg tggcctatac cgcaggccct ggattagtcg gcgcactgct ggttggcgcg
    42481 accgtggggc gttctctggc gtttgcctgg aacgttccgg cgatccctgt acaccatatg
    42541 gaagggcatc tgttagcgcc gatgctggaa gataacccgc cggaatttcc gtttgttgcg
    42601 ctgctggttt ccggcggtca tacgcagtta atcagcgtga ctggcattgg tcagtacgag
    42661 ctgctcggcg agtctatcga tgatgccgcc ggtgaagcgt ttgataaaac cgcgaagctg
    42721 ctggggctgg attatcctgg cggaccgtta ctgtcgaaaa tggcggcgca gggtactgcc
    42781 gggcgctttg ttttcccgcg tccgatgacc gaccgtccgg ggctggattt cagcttctcc
    42841 ggcctgaaaa ccttcgcggc aaataccatt cgtgacaacg gcaccgacga ccagacgcgt
    42901 gctgacatcg cccgcgcctt tgaagatgcg gtggtcgata cgttgatgat taagtgtaag
    42961 cgtgcgctgg atcagacggg ctttaagcga ctggtcatgg cgggcggcgt gagtgctaac
    43021 cgcacgttac gggcgaagct ggctgaaatg atgaaaaaac gccgcggcga agtgttctac
    43081 gcgcgtccgg aattttgtac tgataacggc gcgatgatcg cctatgccgg aatggtgcgg
    43141 tttaaagcag gcgcgacggc ggatctcggc gttagcgtgc gtccgcgctg gccgctggcg
    43201 gagttaccgg ctgcgtaaaa ctatcgggtg ccggagagtt acttccggca ccgtccttac
    43261 ttaaagcaac accacgggca tccacagtaa gccggtaatc accagcaata cgaggaatat
    43321 cagcccaaaa atcgccccca gtcgccagta atccgccgtt ggcagataac cactgccgta
    43381 gtaaatcgga ctggggccgg tggcgtatgg tgtaagaatg ctccccagac caattgccgc
    43441 accaaccatc aggcagaaca ccggcagcgg gatttccggc atcgccagcg cagcggcaat
    43501 catcatcggt gcgagagcgg aggtatacgc cgtggcgctg gcaaaaaagt agcgcagtag
    43561 ataaaacacc acaatcaacg ccaccatcac catcgttggc gaataaccgc ttaagctgcc
    43621 tgctaacagt ttgccaaacc agctaataaa accggtgttg ttgagtccgg tcgcgagggt
    43681 gataagcgag gccagccaga agaaaacgtt ccacgccgct ttattactga caatatcgtc
    43741 ccaactgata atgcgcagaa gcagcatcag cgccaccacg ctgtaaccga ccatcgcggc
    43801 atcgatataa tcaccgccga aaatccacag caccagcgcg cctaccatca gccccagcat
    43861 ccgtttttca cgcgaacaga gcgggcccat tgcctgcagt tccgtctctg cccagcgcgg
    43921 cacctgatcg cctgacttca gtacaggcgg gtacagcacg taagccagcc agggaaccag
    43981 caggaccagt aaaatactga gcggcaacat cccgaggaac cagtcgcccc aactcagcgt
    44041 ggcgtgagat gcgcttttca tcagtccaat taacagcaag ttaggagcca tcgccgtcag
    44101 gaaaatggcg ctggtcacgc agtcggcaac aatccccatc cacatgatgt acgagccaat
    44161 actgcgcgaa ctgctgtcgt ttggttgtga ttgatagagc ggcggcaggt tacggatgat
    44221 gggatagata atccccgcac cacgcgccga gttggacggt gtgacaggtg ccaggatcag
    44281 ctcggagaac atcaccgcat agccaagaaa cagcgtgcga tgccccatct ttttcaccag
    44341 aatcagcgcg atacgtcgcc ccagcccggt tttttcatag cctgtgccaa acataaaggc
    44401 ggcgaaaatc agccagataa ccgaattaga aaaaccggaa acggcccacg agagggattt
    44461 tgcagtgaat ttaaagcctg gctgagcgag ctgctccggg ctgaacagca gccagggaga
    44521 gagtatggcg atgatggaga tacccaccat cgccaccacg gcacccggca cgggttcgag
    44581 gatcagtcca acgattacgc cagtaaaaac ggcaaagtag agccaggtat gactctccag
    44641 ccctgcggga accggaatta gagcaataat ggcgatgacc gccagcggcg caaggtatcg
    44701 ccaccattca gtggaaggtt tcatcgtgtt atgcctgcga aaaaggagag ggggcgagcc
    44761 tctccgggag ggttatttga tgtaatggac gtgctcgcag atctcttcca cgatgggatc
    44821 gcggcgttcg gcgaacagct ttttgttttc ggctatcagg ttgttgccgt gggtatcaat
    44881 agagacaatc agcgggccga actctttgac ccggcagacc cacagtgact ccggcatccc
    44941 gagttctgtc cagtgcactt cttcaatctc ttccacctgg gttgccgcca gcaccgcgca
    45001 gcctgccggg aaaattacat gtagcgcctt gaatttctga cagccttctt cggtcagcgg
    45061 ccccattccg cctttgccga ccaccagttt cacgccggtc tgctcgataa attcacgttc
    45121 aaaactttcc atacgcatac tggttgtcgg accgacggag accatctccc atttgtcgcc
    45181 gtttttgcgc acgatgggac cagcgtggaa aatcgctttg ccgttgagat cgtaagggag
    45241 cggacgtttc agttcgatca atcggcggtg acaaacgtca cggcaggtca ccagcgtacc
    45301 ggtcagatag atcacatcgc cgacgtgaat atcttgcaga tcttcagctt tgatcggggt
    45361 tgtcaggatc tttttcataa cgcgctccgg gtgtgagaca gattttcaaa ggtgagatcg
    45421 gcatgaacca gcagtgtgcc gcgacgatgc gcccagcaac cggtagaaac agcaacgccg
    45481 atggttgacg gatggcgggc ggcagattcg atatgcacgc ccatcactga actgttgcca
    45541 gttagccctt gtggaccaat ccccagacgg ttgagtcctt cttccaggcg tagctccagc
    45601 tctgccgctt ttggattggg gtggcggctg ccaatcgggc gcaaaatggc tttacgcgag
    45661 agtacggcgg cggtttccac cgaggtggcg atgcccacgc ccaccagtac cggtggacag
    45721 gcgtttactg cgagggtgga gatattttcg aagacgaatt tcaccacgcc ttcgtagcct
    45781 tctgacggca ttaacacttt cgagcggcct ggtagcgtgc agccgccgcc tgccatgtaa
    45841 acttcgattt ccgcatcgtc accgtcgggg acgatatccc aggtgaccca cggtacgccg
    45901 ctaccggtat ttttgccggt gtttacttcg tcaaaaattt ctaccgcatt gtgacgcagc
    45961 ggcgctttga tggtcgcctc ttccacggct tgtttgagta tgctttgcag ctcgccaagc
    46021 agtgggaagc gggaaccgac tttaacgaag aacataatct ccccggtgtc ctgacaggca
    46081 ggacgattca ggtcaatcgc tttttgcatg ttgtcgaaca tcgtatggta gataattttc
    46141 cccatcgacg acgtttcggc atcctttagc tgttttagtt tatccaccac gtcatcaggc
    46201 attcgggtag aaatcatggc ggtaaagtta gcgacaatct ccgtcaactt attcactgcc
    46261 tgttgcttat tactttcgct catcatggca actccagttt ttgtatttgt ctggaaaaat
    46321 tcaccgccac aaactaccac taaaaatgtg gttttatatt ttttgccacc gtgaatatgt
    46381 aattaacagt tcgtgaatca acgcgaaaat aagaatgaat attgcgccag agcagggaat
    46441 ggcaatgttt tgcgaaatac actgcgctta tcacaactgc ggataattaa cgatgctgaa
    46501 tagctggcct ttagccaaag acttgcaggt actggtagaa atcgtccaca gcgggagttt
    46561 tagtgctgca gcggcgacgc ttgggcaaac gcctgcattt gtcaccaagc ggatccaaat
    46621 ccttgaaaat acgctggcaa caacgctgct gaaccgctcc gctcgcggcg tggcgctgac
    46681 tgaaagtggc caacgttgtt acgaacatgc gctggaaatc ctcacccaat atcagcgact
    46741 ggtcgatgac gtcacgcaaa tcaaaacgcg cccggagggg atgattcgta ttggttgcag
    46801 ttttggtttt gggcgcagcc atattgcgcc agccattacc gaacttatgc gcaattatcc
    46861 tgagttacag gtgtattttg aattgttcga tcggcaaatt gatttagtgc aggataatat
    46921 tgatctggat attcgtatta atgacgaaat tcctgattat tatattgcgc atctgttaac
    46981 gaaaaataaa agaatattat gcgcagcacc tgagtatctg caaaaatatc cgcagccaca
    47041 atccctacag gaattaagtc gtcatgactg tctggtgacc aaagagcgcg atatgaccca
    47101 cggaatatgg gagttgggga acggtcagga gaaaaaatcg gtgaaggtta gcgggcatct
    47161 ttcctccaat agcggcgaga ttgtgctgca atgggcgctg gagggcaaag ggattatgct
    47221 gcgttccgag tgggatgtgt tgccgtttct ggagagtggc aaactggtgc aagtattgcc
    47281 ggagtatgca caaagcgcca atatctgggc tgtttaccgg gagccgctct atcgcagcat
    47341 gaaattacgt gtctgcgtgg aatttctggc ggcatggtgc cagcaacggc tgggcaaacc
    47401 cgatgaaggc tatcaggtca tgtagatcca ccagaaatca ctcgggatcc ttttcgcgct
    47461 ttcttttgaa tttcgtccag atttttgtct cctgacgacg ccacagacgc tggatgttgt
    47521 catgatgacg cagcaggatc aggcaagaga gcatcgaaac cgggaaggtg aattgtggct
    47581 taaaccacca gacataaaac ggagcaatca gtgcactgac aatcgctccc agcgacgagt
    47641 atccgctcaa tagcacggtc agtaaccagg ttcccgccat tactccggtg agatcccagc
    47701 caatgggtgc gatggcacca aaagcggtag caacgccttt tcctccttta aatccgaaga
    47761 aaacgggcca gatgtgtcca agacaggcgg cgatggcaat taagcctagc cagaaggggc
    47821 tgacacctaa ttcatacgcg ccccagacgg gcaacattcc tttcagaacg tcgaaaatca
    47881 gtactgctac ggctgctcct ttgccaccta tacgtaacac gttagttgcg ccaggattac
    47941 cggagccgct ggttcgcgga tcgggcagcc cacacaggcg gcaaaccaga atggcactgg
    48001 aaatggagcc gcagaggtac gcgatgagga tcattccagg cgcgattgca ctcataagct
    48061 gttccgtttt gaaaattcgt gttaaacgat gaatctgtgg ataatacgca catttcgccg
    48121 gaagtggtat ccgggttagc caaaaagcga gcaggtcgtg atggatattg tatttataga
    48181 gcaactttcg gtaatcacca ctattggtgt ttacgactgg gaacagacca tcgaacagaa
    48241 attagtgttc gatatcgaaa tggcgtggga taaccgtaaa gcggcaaaaa gcgataatgt
    48301 ggcggattgc ctcagctacg ctgacattgc agaaacggtg gtcagccacg tcgagggggc
    48361 acgttttgcg ctggtggagc gcgtggcgga agaggtggca gagctgctgt tagcacgctt
    48421 caactcgccg tgggtgcgta tcaaactcag caagccaggc gcagtggcgc gggcggcgaa
    48481 tgttggcgta atcattgagc gtggcaataa tctgaaagaa aataattaat tttacagctg
    48541 ttaaaccaaa cggttataac ctggtcatac tgcagtagtt cggacaagcg ttacattttt
    48601 ataatttagg ggtttattga tgagcgatat gcactcgctg ctgatagcgg caatattggg
    48661 tgtggtcgaa ggattgacag aatttctgcc ggtatccagc acgggccata tgattattgt
    48721 cggtcacttg ttggggtttg agggcgacac ggcgaaaacc tttgaagttg tgatccagtt
    48781 aggatcaatt ctggcggtag tagtgatgtt ctggcggcgt ctgtttggcc tgattggcat
    48841 ccactttggc cgcccgttgc agcacgaagg agaaagcaaa ggtcgtttaa cgctgatcca
    48901 cattttgctg gggatgattc cggcggtggt actggggctg ttgttccacg acacgattaa
    48961 gtcattgttt aacccgataa atgtgatgta tgcgctggtc gttggcggtt tgttgctgat
    49021 tgccgccgaa tgcctgaagc cgaaagagcc gcgtgcgccg ggtcttgatg atatgaccta
    49081 tcgtcaggcg tttatgattg gttgtttcca gtgtctggcg ctgtggccgg gtttctcccg
    49141 ttccggggcg accatttcag gtgggatgct gatgggggtg agccgttacg ctgcttccga
    49201 gttttcgttc ctgctggcgg tgccgatgat gatgggcgca acggcgctcg atctctacaa
    49261 aagctggggc ttcctgacaa gcggcgatat cccgatgttt gccgttgggt ttatcaccgc
    49321 ttttgtggtg gcgctgatag cgattaaaac cttcctgcaa ttgattaagc gcatttcgtt
    49381 tatcccgttc gccatttatc gctttattgt ggcggctgcg gtgtatgtcg tgttctttta
    49441 attgaggaat agtgtcggat gcctggcgtt gtccacgatt gcgtaggccg aataaggcgt
    49501 tcacgccgcg tccggcaatc aacgcttgat gcgacgcttg cgcgtcttat caggcctaca
    49561 actgtcgttg acatgtagcc ggataaggcg tttacgccgc atccggcaaa atgcccaata
    49621 ctcactcagg ctttgggcaa cgttgttcct tccagctggc taccgccgca atccgccgtc
    49681 gggtcagctc ctcgcgaatc tccacacctt taaatcccgc ttcaacgacg gcttttgtcg
    49741 gcactgactg cgccacttcc caggcttcgc gcaaccagcg gccttgcggg tagtccgctg
    49801 attcaaaacc ggttctgccg cgcacgtcag cctcgctggt cagcgccagt tgctcgacac
    49861 gctgcggttt acgccaggca tcgatggaat caaataattt gacgatggtt tttgggttca
    49921 gcattgggaa ggtgtggatg agatcgtgaa actcagccac cagtctggct aaatcgcgaa
    49981 tttcatttgg cacgcgcaga cgctggcata gttgttccac taacttaaca cccgccgggc
    50041 catgaccatg atgacgcggc cagagttctg gcggcgtcag tcctttaccg agatcgtggc
    50101 ataaagttgt gaaacggaca tcgacctgcg gactcagcat cgccgccatt gagagcgtca
    50161 ttaaggtatg aatacccgtg tcgatttccg gatgccacct ggcaggggcc ggaacaccaa
    50221 acagggcgtc aatttccggg aataaaacgc gcagtgcgcc gcaatcgcgc agtacctgga
    50281 agaacacctg tggattgcgg gtggtgaggg cgttttctgt ctctttccat acccgttcag
    50341 gcgtcaggtg ttccagttca cccgcatggg tcatctcgcg catcagcgtc agagtttcat
    50401 cggcaatacg aaaaccgagg tgggcataac gcgcagcaaa acgcgccacg cgcagtacgc
    50461 gtaacggatc ttcgccaaaa gcgggggaaa catggcgcaa cagacgattt tgcagatcgc
    50521 ccagaccgtt gtacgggtcg ataatctcgc cgttatcgtc ctgggccagc gcattaatgg
    50581 tcagatcgcg acgcttaaga tcatcttcca gcgtgacatc cggtgcggca tagcaggtaa
    50641 aaccggtgta accggagccg gatttccgtt cggtacgtgc cagcgcatac tcttcatgcg
    50701 tttgcggatg cagaaacaca ggaaaatcgc ggcctacctg ctggtagccc gcgtcgagca
    50761 tctcctgtgg cgtactgccg accactaccc aatctctgtc tttgaccggt agccctaaca
    50821 atgcatcccg aacagcacca ccgaccagat aaatcttcac gccatcatcc cttcgtcaaa
    50881 tacacaattc ctaataataa gacagtgcaa cgaagaaggc gatttagttc atccagcgat
    50941 ctttgcgttt gcggcttggg atcaggtgcg gcagtaccag accgagcagc aagccaagcc
    51001 ccagcacgcc gccgccatac ataaaccatt gcatgatgat ggtgcgctgt ttgtcatcca
    51061 gctgcacgct ggcggcatcg acctttttct gcgcgacaat cagctcgttt ttcagtttct
    51121 gattttcttc ttttaacccg ttgatcacgc tgtcgctctg cgccactttt tgctgcattt
    51181 ctgcggtgcg ctgattccag gtgttatcga tattggtgag tttatcggtc agggttttga
    51241 cctgattttc cagatctggc acacgggagc gcaggcttgg ctcagtgcta agttgtttca
    51301 acgggatcca ggcggtacgg ccagagctgt ctttcacctg ggcataattg gtgttggcgt
    51361 cagtttgtaa taaggtcact tcctcgccgg cgttaaccgt gcccacgagg cgataatgat
    51421 ctcccggacc gctacggacc caggtattca gttcgtcaga aacatagcgc gtttcttcag
    51481 cgtgtgagac ggcagtcgcg ctaagtgcga gtaaagttaa tccgatcagg cgtaattttg
    51541 gcatcaggct gtcgttattg tcatggaaag tggaacgata gtagtggcat cagtgtcgct
    51601 acgcaaagca ttcgacatca atcggaatcc tctgtgtcag gtcttgccac tgcttcaaac
    51661 ttttacgccc gtcaaattgc ccgttgcatg gcaatttggc gcaaaatact atctactgac
    51721 aaaaaagatc gcattaagtt cgccgttcat cgtcacttat gtgacataac cgataagtaa
    51781 aggccatggc tcaggaaatc gaattaaagt ttattgttaa tcacagcgcc gttgaggcgt
    51841 tgcgtgacca tctcaatacg ctgggcggcg agcaccatga ccccgtgcag ttactgaata
    51901 tttactacga aacgccggat aactggctgc gtgggcacga tatgggctta cgtattcgtg
    51961 gcgaaaacgg tcgctatgag atgaccatga aagttgcagg aagagtgaca ggcggcttac
    52021 accagcgccc ggaatataac gtggcgttga gcgaaccgac gctcgacctg gcgcagttac
    52081 cgacggaagt ctggccgaac ggcgaattgc ccgccgatct cgcttcccgc gtacaaccgc
    52141 tgttcagcac cgatttttat cgcgaaaaat ggctggtggc ggtcgatggc agccgaattg
    52201 aaatcgctct cgatcagggt gaggtgaaag cgggggaatt tgctgaacct atctgtgagc
    52261 tggaactgga gctgcttagc ggcgacacgc gcgcggtgct gaaactggcg aaccaactgg
    52321 tatcgcaaac cggattacgc cagggcagcc tgagcaaagc ggcgcgtggc tatcatctgg
    52381 cgcagggcaa tccggcgcgt gaaatcaaac cgaccaccat tttgcatgtt gcggcaaaag
    52441 ccgatgtgga acaggggctg gaagcggcgc tcgagctggc gttagcgcaa tggcagtatc
    52501 atgaagaact gtgggtacgc ggcaacgatg cggcgaaaga acaggtgctg gcagccatta
    52561 gcttggtccg tcatacgctg atgctgttcg gtggtattgt gccgcgtaaa gcgagcactc
    52621 acttacgtga tttgctgacc caatgcgaag cgaccattgc ttctgcggtt tctgccgtga
    52681 cggcggtcta ctctaccgaa acggcaatgg cgaagctggc gttgaccgaa tggttggtaa
    52741 gcaaagcatg gcagccattt ttagatgcca aagcgcaggg caaaatcagc gactccttca
    52801 aacgctttgc cgatatccat ctttcccgcc atgccgctga actgaaaagc gttttctgcc
    52861 agccgttagg cgatcgctac cgtgaccagt tgccacgcct gacgcgtgat attgactcaa
    52921 tactgttgct ggcgggttac tatgatcctg tcgtcgcgca agcctggctg gagaactggc
    52981 aggggctgca tcacgctatt gcgaccgggc aacgcatcga aattgaacat ttccgtaatg
    53041 aggcaaacaa tcaggaaccg ttctggttgc acagcggaaa acgttaatca ggcaaggata
    53101 aaatcgctta tgaagccgct ctcttcaccg ttacagcagt actggcagac cattgttgag
    53161 cggctgccag agcctttagc cgaggaatca cttagtgcac aggcgaagtc agtacttact
    53221 ttcagtgatt ttgtgcagga cagcataagt gcgcatccag agtggctgac ggaactggaa
    53281 agccaaccgc cgcaggccga cgaatggcag cattacgttg catggttgca ggaggcgctc
    53341 attaatgtga gtgacgaagc cgggttaatg cgcgagctgc ggctgttccg tcggcgcatc
    53401 atggtacgta tcgcctgggc gcaaacgctg gcactggtta ctgaagagag catattgcag
    53461 cagctcagct atctggcgga gacgctgatt gttgcggcgc gtgactggct atatgacgcc
    53521 tgctgccgcg agtggggaac gccgtgcaat gcgcagggcg aagcgcaacc gctgctgatt
    53581 ttaggcatgg gtaagctggg cggcggggaa ctgaatttct cctctgatat cgatctgatt
    53641 tttgcctggc cggaacatgg ttgtacgcag ggtggacgct gggaactgga taacgcgcag
    53701 ttttttaccc gcatggggca gcggctgatt aaagtgctgg atcaaccaac gcaggatggc
    53761 ttcgtctatc gcgtggatat gcggctgcgt ccgtttggcg aaagtggccc gctggtgctg
    53821 agctttgccg cgttggaaga ttattaccag gagcaggggc gcgactggga gcgttacgcg
    53881 atggtcaagg cgcggattat gggcgatagc gaaggcgtct atgctaacga gttgcgtgcg
    53941 atgctgcgcc cgtttgtttt ccgtcgttac atcgatttca gcgtgattca gtcgctgcgc
    54001 aacatgaaag gaatgattgc ccgtgaagtg cgtcgacgtg gtttgaccga caatatcaaa
    54061 ctcggcgcag gcggcattcg cgaaattgaa tttatcgttc aggtgttcca gctcattcgc
    54121 ggcgggcgcg aaccgtcgct gcagtcgcgc tctttactgc caacgctcag cgtcattgcc
    54181 gcgctgcatc tgctttctga aaacgatgct gaacaattgc gagtggcgta tctgttcctg
    54241 cggcgtctgg aaaacctgct gcaaagcatt aacgacgaac aaacccagac gcttccttct
    54301 gatgagctta accgtgcgcg gctggcgtgg gcgatggact ttgctgactg gccgcaattg
    54361 accggggcgc tgaccgcaca tatgaccaat gtgcgccggg tgtttaatga attgattggc
    54421 gacgacgaaa gtgaaactca ggaagagtcg ctgtcggaac agtggcgcga gctgtggcag
    54481 gatgcgttgc aggaagatga cactacacca gtgctggcgc atcttagcga ggacgatcgc
    54541 aaacaggtgc tgacgctgat tgccgatttc cgcaaagagc tggataagcg cacaatcggg
    54601 ccacgcgggc gtcaggtgct cgaccatctg atgccgcatc tgctaagtga tgtctgtgcg
    54661 cgtgaagacg ctgccgttac gctgttgcgc attaccgcct tgctggcggg gattgttacc
    54721 cgcaccacct atttagaatt gctcagtgaa ttccccgcgg cgcttaaaca tttgatttct
    54781 ctgtgtgcag cgtcgccgat gattgccagc cagctggcgc gttatccatt attgctggat
    54841 gaattgctcg atccaaacac cctttaccag ccgacggcga ccgatgccta ccgcgatgag
    54901 ttgcgccagt atttgctgcg cgtgccggaa gatgacgaag agcaacagct tgaggcgctg
    54961 cgtcagttca aacaggcgca gctgttacgc atcgccgcag cggatatcgc cggtacgcta
    55021 ccggtgatga aagtgagcga tcacttaacc tggctggcgg aagccatgat agatgccgtc
    55081 gttcagcagg cgtgggttca aatggttgcc cgctacggta agccgaatca cctgaacgaa
    55141 cgcgaagggc gtggttttgc agtggtcggc tacggcaagc tgggcggctg ggagttaggc
    55201 tacagttccg atcttgacct gatcttcctc catgattgcc caatggatgc gatgactgac
    55261 ggcgagcggg aaatcgacgg acgacagttt tatctgcgtc tggcgcaacg cattatgcac
    55321 ctgttcagca cgcgaacctc ttccggcatt ttgtacgaag tggatgcgcg gctgcgcccg
    55381 tccggggcgg cgggaatgct ggttacctcc acagaagcat ttgccgatta tcagaaaaac
    55441 gaggcctgga cgtgggagca tcaggcgctg gtgcgtgcgc gtgtagtgta cggcgatccg
    55501 cagctcaccg cgcactttga cgcagtgcgt cgcgagatta tgacgctgcc gcgtgaaggt
    55561 aaaactctgc aaacggaagt gcgggaaatg cgcgagaaaa tgcgcgctca tctcggcaat
    55621 aaacatcgcg atcgctttga tatcaaagct gatgaagggg gaattaccga tatcgaattt
    55681 attacccaat atctggtgtt gcgctacgct catgaaaaac cgaagttaac gcgctggtca
    55741 gacaacgtgc gcattctgga actactggcg caaaacgaca ttatggaaga gcaggaagcg
    55801 atggcgctga cccgtgctta cactacgctt cgcgatgaac ttcatcatct ggcattacag
    55861 gaattgccgg gccatgtgcc ggaggattgc ttcaccgcag agcgtgaact ggtgcgggca
    55921 agctggcaga agtggctggt ggaagaatga agtatggtat tatcgcgcgc aaattttgaa
    55981 tctctcagga gacaggaatg aaagtaacgc tgccagagtt tgaacgtgca ggagtgatgg
    56041 tggttggtga tgtgatgctg gatcgttact ggtacggccc ctccagtcgt atctcgccgg
    56101 aagcgccggt gcccgtggtt aaagtgaata ccatcgaaga acgtccgggc ggcgcggcta
    56161 acgtggcgat gaatatcgct tctctcggtg ctaatgcacg cctggtcggg ttgacgggca
    56221 ttgacgatgc agcgcgcgcg ctgagtaaat ctctggccga cgtcaacgtc aaatgcgact
    56281 tcgtttctgt accgacgcat ccgaccatta ccaaattacg ggtactttcc cgcaaccaac
    56341 agctgatccg tctggatttt gaagaaggtt tcgaaggtgt tgatccgcag ccgctgcacg
    56401 agcggattaa tcaggcgctg agttcgattg gcgcgctggt gctttctgac tacgccaaag
    56461 gtgcgctggc aagcgtacag cagatgatcc aactggcgcg taaagcgggt gttccggtgc
    56521 tgattgatcc aaaaggtacc gattttgagc gctaccgcgg cgctacgctg ttaacgccga
    56581 atctctcgga atttgaagct gttgtcggta aatgtaagac cgaagaagag attgttgagc
    56641 gcggcatgaa actgattgcc gattacgaac tctcggctct gttagtgacc cgttccgaac
    56701 agggtatgtc gctgctgcaa ccgggtaaag cgccgctgca tatgccaact caggcgcagg
    56761 aagtgtatga cgttaccggt gcgggcgaca cggtgattgg cgtcctggcg gcaacgctgg
    56821 cagcgggtaa ttcgctggaa gaagcctgct tctttgccaa tgcggcggct ggtgtggtgg
    56881 tcggcaaact ggggacgtcc acggtttcgc cgatcgagct ggaaaacgct gtacgtggac
    56941 gcgcagatac cggctttggc gtgatgaccg aagaggaact gaagctggct gtagtggcag
    57001 cgcgtaaacg tggtgaaaaa gtggtaatga ccaacggtgt cttcgatatc ctacatgccg
    57061 ggcacgtctc ttatctggca aatgcccgca agctgggtga ccgcttgatt gttgccgtca
    57121 acagcgatgc ctccaccaaa cggctgaaag gggattcccg cccggtaaac ccactcgaac
    57181 agcgtatgat tgtgctgggc gcactggaag cggtcgactg ggtagtgtcg tttgaagaag
    57241 acacgccgca gcgtctgatc gccgggatcc tgccagacct gctggtgaaa ggcggtgact
    57301 acaaaccgga agagatcgcc gggagtaaag aagtctgggc caacggcggc gaagtgttgg
    57361 tgctcaactt tgaagacggt tgctcgacca ctaacattat caagaagatc caacaggata
    57421 aaaaaggcta accggaaagc ggttcacaga tctcgcttcg tacatggcag gcctattgac
    57481 gctgttgtct tttcaaccta gagtaaagga acaagggtaa gggaggattt ctcccccctc
    57541 tgatgagttg ttagtaagtc gggaaactta acagtaacaa cacaaccagt atgatgacga
    57601 gcttcatcat aaccctttcc ttctataagg ccccttcctc gggaggggct ttcccgtttc
    57661 agccctttgc ttactccccg attgttgatg agcgccggac cccgcgcaac gctttccctg
    57721 aagcagtttt gccttccccg agcggtcacg caaaacgctg caacgtcctg agcatttgca
    57781 ccttttagcg gaacgcagat ataaaaaatc cccggctttt tgggccaggg atatgtgtta
    57841 atgaaacctt cttgacgctg attttttttc aacctaaagt aaaggaacaa gggtgaggga
    57901 ggatttctcc cccctctgat tggctgttaa taagctgcga aacttacgag taacaacaca
    57961 atcagtatga tgacgagctt catcataacc ctttccttct gtaaggcccc ttcctcggga
    58021 ggggctttcc cgtttcagcc ctttgcttac tccccgattg ttgatgagcg ccggaccccg
    58081 cgcaacgctt tccctgaagc agttttgcct tccccgagca atcacgcaaa acgctgcaac
    58141 agcctgatca tttgcacctt ttagcggaac gcagatataa aaaatccccg gctttttgag
    58201 ccagggatgt tcatagctga aaatttattc tacgttttcg gcgacgggag ttgttggggt
    58261 taagagtgaa gtcaatgcag ccagtgagcc gcctgaaacg ccaatttcat tgagcaagga
    58321 gtcaatcagt ggtgcctgtg tgcggtaaga gagtgcggct gataatgctt gttccgccag
    58381 gtttccgccc cccacattac ccgtgtttgc atcccccgca gtaccaccac gattcagacc
    58441 atcgacctga ataatcttga taccgtcaat ggacttcatt ggttccacgg atttctctat
    58501 taccgcaggt agcgcctgca acaaggccag tttgaattta aggctggttt gttcgtcaga
    58561 aagtacgttg atagcatcgt tcagcgcacg ttgtgcttct gcttccgcca ggcctttttt
    58621 acgcgtagct tcggctaact caacgatcgc cgccgcctgc atctctgcgg cttctttttc
    58681 tgctttcgcc cgcacggtca gttcaaccgc tttggtttct gcatcctgtg ctgcggcaat
    58741 cagcgccacc tgcttggcac gatcggcttc ggctgtctgg cgagtggttt ctacgttttg
    58801 ttgggcgctt actgcttctg cgagtgcaag gttagcacgc gcttctgcct gggactgttg
    58861 ttccgatttg gcggcaatag cgatcgattt cgtctggtta gcgatttcgg tgacctgctg
    58921 ctgttcgatc tctttaatgc gaacttcacg ttcagcttca acttttcttg agcggacggc
    58981 ctgttcgcgg tcgatttctg tttcctgaat ctgtcgttca gccagaattc gcgtctgctc
    59041 tgcttcacga cgacgttcag cttcaaaagc cgcaattttc gcattctgct cagcggtacg
    59101 ggttttaacc tgctgctcct gctcaagcgt cataaacgct tcttgttgtt caatcttcaa
    59161 cttgcgcgaa agtgcatcac ggtttttctc acgcaccgca acttctacat cctgttcaac
    59221 ttcgttacgc tcgcggcgac ggcgctccgt ctcctgagtc agtttggtta aaccttcggc
    59281 gtcaaaggca ttgttaggat tgaaatgttc tttcgaggtc tggttaaagt tggtaagtga
    59341 aacgctttcc agttccaggc cgtttttcga cagatcttca gctacggtat tttgtactcc
    59401 ctgcacgaag ttctcgcggg tatcctgtaa ctcatgcatg gtcatttgcg cagctgttgc
    59461 acggagggca tcgacaaatt tatcttcaac caacatacgt aagtcttcag gcgacagggt
    59521 gcgttgcccc agcgtctggg cagcggtggc aatcccttct actgaaggtt ttacccgcac
    59581 aaagaaagcg actactacat cgacgcgcat acgatctttc gtaatcaggc tatcaatggt
    59641 tgagcggctg acttccagct tcagagtatt catattgatg gggattattt catgaaagat
    59701 cggcatcacg attgcgccac cgctcattac cactttttgc ccccctaagc cggtacggac
    59761 aaatgcttgc tctgccgaag cgcgacgata gagcctggcg aaaataattc caataataaa
    59821 cagaatgcat acggcaataa tcgcggtaaa catccaggag ggcacggaat taacaatatc
    59881 atccattatt aaatttcct
//
LOCUS       DBFIFI010000010        58790 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.010, whole genome shotgun sequence.
ACCESSION   DBFIFI010000010 DBFIFI010000000
VERSION     DBFIFI010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58790
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.010"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..185
                     /locus_tag="N7B39_000608"
     CDS             <1..185
                     /locus_tag="N7B39_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGU2420647.1"
                     /translation="TPDEQKEPVAAIAPSLSTSTQTRVSASSCKVEFRHGNMTLENPS
                     PELLTMLIRELSGRGR"
     gene            182..532
                     /gene="tnpB"
                     /locus_tag="N7B39_000609"
     CDS             182..532
                     /gene="tnpB"
                     /locus_tag="N7B39_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311895.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGU2420648.1"
                     /translation="MISLPSGTRIWLVAGVTDMCKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            563..2148
                     /locus_tag="N7B39_000610"
                     /pseudo
     CDS             563..2148
                     /locus_tag="N7B39_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            2177..2320
                     /locus_tag="N7B39_000611"
                     /pseudo
     CDS             2177..2320
                     /locus_tag="N7B39_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2431..2738
                     /locus_tag="N7B39_000612"
                     /pseudo
     CDS             2431..2738
                     /locus_tag="N7B39_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612262.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2735..3970)
                     /gene="pitB"
                     /locus_tag="N7B39_000613"
                     /pseudo
     CDS             complement(2735..3970)
                     /gene="pitB"
                     /locus_tag="N7B39_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
     gene            complement(4262..6121)
                     /gene="gss"
                     /locus_tag="N7B39_000614"
     CDS             complement(4262..6121)
                     /gene="gss"
                     /locus_tag="N7B39_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HGU2420649.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKE"
     gene            6326..7192
                     /gene="yghU"
                     /locus_tag="N7B39_000615"
     CDS             6326..7192
                     /gene="yghU"
                     /locus_tag="N7B39_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HGU2420650.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(7315..7563)
                     /gene="hybG"
                     /locus_tag="N7B39_000616"
     CDS             complement(7315..7563)
                     /gene="hybG"
                     /locus_tag="N7B39_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HGU2420651.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(7576..7917)
                     /gene="hypA"
                     /locus_tag="N7B39_000617"
     CDS             complement(7576..7917)
                     /gene="hypA"
                     /locus_tag="N7B39_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HGU2420652.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEVHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(7910..8398)
                     /gene="hybE"
                     /locus_tag="N7B39_000618"
     CDS             complement(7910..8398)
                     /gene="hybE"
                     /locus_tag="N7B39_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HGU2420653.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHALSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(8391..8885)
                     /gene="hybD"
                     /locus_tag="N7B39_000619"
     CDS             complement(8391..8885)
                     /gene="hybD"
                     /locus_tag="N7B39_000619"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HGU2420654.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKSAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(8885..10588)
                     /gene="hybC"
                     /locus_tag="N7B39_000620"
     CDS             complement(8885..10588)
                     /gene="hybC"
                     /locus_tag="N7B39_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HGU2420655.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(10585..11763)
                     /gene="hybB"
                     /locus_tag="N7B39_000621"
     CDS             complement(10585..11763)
                     /gene="hybB"
                     /locus_tag="N7B39_000621"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HGU2420656.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(11753..12739)
                     /gene="hybA"
                     /locus_tag="N7B39_000622"
     CDS             complement(11753..12739)
                     /gene="hybA"
                     /locus_tag="N7B39_000622"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HGU2420657.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(12742..13860)
                     /gene="hybO"
                     /locus_tag="N7B39_000623"
     CDS             complement(12742..13860)
                     /gene="hybO"
                     /locus_tag="N7B39_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HGU2420658.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(14049..14336)
                     /gene="yghW"
                     /locus_tag="N7B39_000624"
     CDS             complement(14049..14336)
                     /gene="yghW"
                     /locus_tag="N7B39_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HGU2420659.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(14455..15342)
                     /gene="yghX"
                     /locus_tag="N7B39_000625"
     CDS             complement(14455..15342)
                     /gene="yghX"
                     /locus_tag="N7B39_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HGU2420660.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIFAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLNSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRILKWFDKYLS"
     gene            15499..16539
                     /gene="gpr"
                     /locus_tag="N7B39_000626"
     CDS             15499..16539
                     /gene="gpr"
                     /locus_tag="N7B39_000626"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HGU2420661.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPEDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDERVTSVLVGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(16579..17073)
                     /gene="yqhA"
                     /locus_tag="N7B39_000627"
     CDS             complement(16579..17073)
                     /gene="yqhA"
                     /locus_tag="N7B39_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HGU2420662.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            17264..18148
                     /gene="yghA"
                     /locus_tag="N7B39_000628"
     CDS             17264..18148
                     /gene="yghA"
                     /locus_tag="N7B39_000628"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HGU2420663.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQQTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(18420..18845)
                     /gene="exbD"
                     /locus_tag="N7B39_000629"
     CDS             complement(18420..18845)
                     /gene="exbD"
                     /locus_tag="N7B39_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HGU2420664.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(18852..19586)
                     /gene="exbB"
                     /locus_tag="N7B39_000630"
     CDS             complement(18852..19586)
                     /gene="exbB"
                     /locus_tag="N7B39_000630"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HGU2420665.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            19838..21025
                     /gene="metC"
                     /locus_tag="N7B39_000631"
     CDS             19838..21025
                     /gene="metC"
                     /locus_tag="N7B39_000631"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HGU2420666.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            21165..21824
                     /gene="yghB"
                     /locus_tag="N7B39_000632"
     CDS             21165..21824
                     /gene="yghB"
                     /locus_tag="N7B39_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HGU2420667.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(21864..22763)
                     /gene="yqhC"
                     /locus_tag="N7B39_000633"
     CDS             complement(21864..22763)
                     /gene="yqhC"
                     /locus_tag="N7B39_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HGU2420668.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMARPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            22957..24120
                     /gene="yqhD"
                     /locus_tag="N7B39_000634"
     CDS             22957..24120
                     /gene="yqhD"
                     /locus_tag="N7B39_000634"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311921.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HGU2420669.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLGVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            24225..25052
                     /gene="dkgA"
                     /locus_tag="N7B39_000635"
     CDS             24225..25052
                     /gene="dkgA"
                     /locus_tag="N7B39_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HGU2420670.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     FDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHNRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            25252..26174
                     /gene="yqhG"
                     /locus_tag="N7B39_000636"
                     /pseudo
     CDS             25252..26174
                     /gene="yqhG"
                     /locus_tag="N7B39_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
     gene            26223..26480
                     /gene="yqhH"
                     /locus_tag="N7B39_000637"
     CDS             26223..26480
                     /gene="yqhH"
                     /locus_tag="N7B39_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HGU2420671.1"
                     /translation="MKTIFTVGAVVLATCVLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(26523..28742)
                     /gene="ygiQ"
                     /locus_tag="N7B39_000638"
     CDS             complement(26523..28742)
                     /gene="ygiQ"
                     /locus_tag="N7B39_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HGU2420672.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQCQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(28853..30265)
                     /gene="ftsP"
                     /locus_tag="N7B39_000639"
     CDS             complement(28853..30265)
                     /gene="ftsP"
                     /locus_tag="N7B39_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HGU2420673.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(30340..31077)
                     /gene="plsC"
                     /locus_tag="N7B39_000640"
                     /gene_synonym="parF"
     CDS             complement(30340..31077)
                     /gene="plsC"
                     /locus_tag="N7B39_000640"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HGU2420674.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(31311..33569)
                     /gene="parC"
                     /locus_tag="N7B39_000641"
     CDS             complement(31311..33569)
                     /gene="parC"
                     /locus_tag="N7B39_000641"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HGU2420675.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(33707..35314)
                     /gene="ygiS"
                     /locus_tag="N7B39_000642"
     CDS             complement(33707..35314)
                     /gene="ygiS"
                     /locus_tag="N7B39_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGU2420676.1"
                     /translation="MYTRNLLWLVSLVSAAPLYAADVPTNTQLAPQQVFRYNNHSDPG
                     TLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQPAQAERWEILDGGRRYIFHLRSGLQ
                     WSDGQPLTAEDFVLGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGVKAT
                     DDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDQWV
                     VNEKITARKNPKYRDAQHTVLQQVEYLALDNSVTGYNRYRAGEVDLTWVPAQQIPAIE
                     KSLPGEIRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATT
                     LTPPEVKGFSATTFDELQKPMSERVAMAKALLKQAGYDASHPLRFELFYNKYDLHEKT
                     AIALSSEWKKWLGAQVTLRTMEWKTYLDARRAGDFMLSRQSWDATYNDASSFLNTLKS
                     DSEENVGHWKNAQYDALLNQAMQITDATKRNALYQQAEVIVNQQAPLIPIYYQPLIKL
                     LKPYVGGFPLHNPQDYVYSKELYIKAH"
     gene            complement(35423..35905)
                     /gene="ygiV"
                     /locus_tag="N7B39_000643"
     CDS             complement(35423..35905)
                     /gene="ygiV"
                     /locus_tag="N7B39_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HGU2420677.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCRPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETNVYVPLA
                     "
     gene            complement(35958..36350)
                     /gene="ygiW"
                     /locus_tag="N7B39_000644"
     CDS             complement(35958..36350)
                     /gene="ygiW"
                     /locus_tag="N7B39_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HGU2420678.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            36502..37161
                     /gene="qseB"
                     /locus_tag="N7B39_000645"
     CDS             36502..37161
                     /gene="qseB"
                     /locus_tag="N7B39_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HGU2420679.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALEERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            37158..38507
                     /gene="qseC"
                     /locus_tag="N7B39_000646"
     CDS             37158..38507
                     /gene="qseC"
                     /locus_tag="N7B39_000646"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HGU2420680.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGDKDQWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPVMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNVQGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            38617..39198
                     /gene="mdaB"
                     /locus_tag="N7B39_000647"
     CDS             38617..39198
                     /gene="mdaB"
                     /locus_tag="N7B39_000647"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HGU2420681.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            39229..39543
                     /gene="ygiN"
                     /locus_tag="N7B39_000648"
     CDS             39229..39543
                     /gene="ygiN"
                     /locus_tag="N7B39_000648"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HGU2420682.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(39588..40475)
                     /locus_tag="N7B39_000649"
     CDS             complement(39588..40475)
                     /locus_tag="N7B39_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HGU2420683.1"
                     /translation="MIQKDKTRVDIYGERFRTRASQLTPGLRAVASYINEHREVVLDQ
                     TAMEIAATLNTSDATVIRAIQALGFAGLRDLKRTLEQWLGPALSSSEKMSTTVSNLTS
                     DVNTAIDFVLEGHLYTCNVLSEPENRHALAQAVALLVQARQVAIFGIGASGILADYTA
                     RLFNRIGLPATALNRTGIGLAEQLIALQRGDVLIMMAQKSAHREGLTTLREARRLGIP
                     VILLTNALDSRFSKDASIVIHVPRGDEKGKTPLHGTVLLCLEMIVWSVASAVPQRAVK
                     TIKRINDFHRGLKTGRKNG"
     gene            complement(40472..41419)
                     /locus_tag="N7B39_000650"
     CDS             complement(40472..41419)
                     /locus_tag="N7B39_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HGU2420684.1"
                     /translation="MRLFFSLLILLSFFARATEPVQVFTDDLGRKVTVPAHPKRIVSL
                     HDLDITIPLIELGVPPVASHGRTRPDGSHFIRSGALLTGVDFDNSSIAFIGTADIDIE
                     AIVAAKPDLIITEPTRNTPIERLEKIAPTVSIDHLKGGAPEIYRKLAELTGTQSQLAI
                     LERRYQAQINALKATLDSQKITVSVIQANQGKINVMHSYHSLGRVLRDAGFRFPPLIE
                     SIPEGGRMDVSAERLPELDADFVFATWRGDTGGKPQDELAAMEKVMPGWCQFLTACRS
                     GRYVLISREEAISNSFASLGLMAAQIQSQIAGRPLPEAK"
     gene            complement(41430..42467)
                     /locus_tag="N7B39_000651"
     CDS             complement(41430..42467)
                     /locus_tag="N7B39_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HGU2420685.1"
                     /translation="MNVGLRPLRVGKFSTLVRPKNLVLLGGLFLFAVGILIFGLMHGS
                     FFVPASEVGRALFAPENVSTDARYIVQDIRLPRVIMALLCGAMLGMAGAAMQSIARNG
                     LADPGLIGVKEGCSVAVLWLIFQFPMLGMFWRPVAGLAGGLLVALIVIFCAREISRPR
                     FVLIGIGVSWFFAAGIGVFMTTADVRDVQTALMWLSGSLHAANWMLVGISACWMLPAA
                     LLLLFTARTADIALLGHQVATGLGVNSSRLALLRVAAPIILTAVCVSCVGNIGFVGLI
                     APHISRFILRGGQTTLLLGSAVSGALLVILADSIGRLAFLPLQLPAGIIISLIGGPFF
                     LLLLWQRRNSF"
     gene            complement(42476..43459)
                     /locus_tag="N7B39_000652"
     CDS             complement(42476..43459)
                     /locus_tag="N7B39_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HGU2420686.1"
                     /translation="MMRTGLVTVIFLALLLVGCVVYPGIGARFIAPQTVLQAFLHFEP
                     QNFDHNVIVRLRLPRLAAALLTGASLGVAGALLQAVIRNPLGEPHILGLNAGAALAVV
                     AASALGLAFPVGRPLLASTGGALLFLLILLLSSAGRSGLTPMKVTLCGVALSAFVSSI
                     TAAILILDEQTLLAMRTWLAGDLAGQDWATLGTSAWFSLGGVVLAIYLAPSLNMLALG
                     DRMAQGLGVSVLRTRTFTLLAIALLCGAAVSIAGPIGFVGLLVPQIVRRLVSADLRVL
                     LPLSACVGALLLLLADIIARTLFTPHELATGVMTALVGAPVFVIMATRMFK"
     gene            complement(43456..44265)
                     /locus_tag="N7B39_000653"
     CDS             complement(43456..44265)
                     /locus_tag="N7B39_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU2420687.1"
                     /translation="MAHNAKGQGLILDNVSAGYHKKIIVDGVSFSVPTEKMTVLVGAN
                     GCGKSTLLSTIARVLQPMGGSILLDGKAIHEQPTKALSRRLGILPQSPLLPEGLTVYE
                     LVSRGRFPWQNFIRQWSDADEQAVEEALKLTGTQEFAHLPVEKLSGGQRQRCWIAMVL
                     AQKTPYILLDEPTTWLDLRYQVEILELLHDLTRHHGRTVVVVLHDLNFAVNYGDTLIF
                     LRQGKVVRVLNEGEHCTPELVKAVFDVDVHASINTLTGKPFFMPFRGVEKV"
     gene            44639..46780
                     /locus_tag="N7B39_000654"
     CDS             44639..46780
                     /locus_tag="N7B39_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HGU2420688.1"
                     /translation="MAKFTPSFSGIKGRALFSLLFAAPMIHATDTATTKDGETITVTA
                     DANTATEATDGYQPLSTSTATLTDMPMLDIPQVVNTVSDQVLENQNATTLDEALYNVS
                     NVVQTNTLGGTQDAFVRRGFGANRDGSIMTNGLRTVLPRSFNAATERVEVLKGPASTL
                     YGILDPGGLINVVTKRPEKTFHGSVSATSSSFGGGTGQLDITGPIEGTQLAYRLTGEV
                     QDEDYWRNFGKERSTFIAPSLTWFGDNATVTMLYSHRDYKTPFDRGTIFDLTTKQPVN
                     VDRKIRFDEPFNITDGQSDLAQLNAEYHLNSQWTARFDYSYSQDKYSDNQARVTAYDA
                     TTGTLTRRVDATQGSTQRMHSTRADLQGNVDIAGFYNEILGGVSYEYYDLLRTDMIRC
                     KNAKDFNIYNPVYGNTSKCTTVSASDSDQTIKQESYSAYAQDALYLTDNWIAVAGIRY
                     QYYTQYAGKGRPFNVNTDSRDEQWTPKLGLVYKLTPSVSLFANYSQTFMPQSSIASYI
                     GDLPPESSNAYEVGAKFELFDGITADIALFDIHKRNVLYTESIGDETIAKTAGRVRSR
                     GVEVDLAGALTENINIIASYGYTDAKVLEDPDYAGKPLPNVPRHTGSLFLTYDIHNMP
                     GNNTLTFGGGGHGVSRRSATNGADYYLPGYFVADAFAAYKMKLQYPVTLQLNVKNLFD
                     KTYYTSSIATNNLGNQIGDPREVQFTVKMEF"
     gene            complement(46844..48736)
                     /gene="parE"
                     /locus_tag="N7B39_000655"
     CDS             complement(46844..48736)
                     /gene="parE"
                     /locus_tag="N7B39_000655"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HGU2420689.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGNCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(48765..49346)
                     /gene="yqiA"
                     /locus_tag="N7B39_000656"
     CDS             complement(48765..49346)
                     /gene="yqiA"
                     /locus_tag="N7B39_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HGU2420690.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(49346..50173)
                     /gene="cpdA"
                     /locus_tag="N7B39_000657"
     CDS             complement(49346..50173)
                     /gene="cpdA"
                     /locus_tag="N7B39_000657"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HGU2420691.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(50198..50620)
                     /gene="yqiB"
                     /locus_tag="N7B39_000658"
     CDS             complement(50198..50620)
                     /gene="yqiB"
                     /locus_tag="N7B39_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HGU2420692.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(50621..51250)
                     /gene="nudF"
                     /locus_tag="N7B39_000659"
     CDS             complement(50621..51250)
                     /gene="nudF"
                     /locus_tag="N7B39_000659"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HGU2420693.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            51455..52936
                     /gene="tolC"
                     /locus_tag="N7B39_000660"
     CDS             51455..52936
                     /gene="tolC"
                     /locus_tag="N7B39_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HGU2420694.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTASS
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            53084..53755
                     /gene="ygiB"
                     /locus_tag="N7B39_000661"
     CDS             53084..53755
                     /gene="ygiB"
                     /locus_tag="N7B39_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HGU2420695.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            53761..54921
                     /gene="ygiC"
                     /locus_tag="N7B39_000662"
     CDS             53761..54921
                     /gene="ygiC"
                     /locus_tag="N7B39_000662"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HGU2420696.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(54959..55747)
                     /gene="ygiD"
                     /locus_tag="N7B39_000663"
     CDS             complement(54959..55747)
                     /gene="ygiD"
                     /locus_tag="N7B39_000663"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HGU2420697.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQTLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            55890..56663
                     /gene="zupT"
                     /locus_tag="N7B39_000664"
     CDS             55890..56663
                     /gene="zupT"
                     /locus_tag="N7B39_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HGU2420698.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(56721..56891)
                     /gene="yqiD"
                     /locus_tag="N7B39_000665"
     CDS             complement(56721..56891)
                     /gene="yqiD"
                     /locus_tag="N7B39_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HGU2420699.1"
                     /translation="MFIAWYWIVLIVLVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(57153..57806)
                     /gene="ribB"
                     /locus_tag="N7B39_000666"
     CDS             complement(57153..57806)
                     /gene="ribB"
                     /locus_tag="N7B39_000666"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HGU2420700.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(57910..58058)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            58180..58479
                     /gene="ubiK"
                     /locus_tag="N7B39_000667"
     CDS             58180..58479
                     /gene="ubiK"
                     /locus_tag="N7B39_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311957.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HGU2420701.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRSSELEARNNSVADLQSPPAIPPIDKAE"
     gene            complement(58514..58714)
                     /gene="glgS"
                     /locus_tag="N7B39_000668"
     CDS             complement(58514..58714)
                     /gene="glgS"
                     /locus_tag="N7B39_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HGU2420702.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQTRELELEH"
BASE COUNT        14838 a        15648 c        14664 g        13640 t
ORIGIN      
        1 taacgccgga tgagcagaaa gaacctgtgg cggccattgc gccgtcttta tccacttcca
       61 ctcagaccag agtcagtgcc agttcctgca aggtggaatt ccgtcacggt aacatgacgc
      121 tggaaaatcc atcgccagag ctgctcacga tgttaatccg cgaactgagc gggaggggaa
      181 gatgatttca ctcccatcag gtacccgtat ctggctcgtt gccggcgtta ccgatatgtg
      241 taaatccttc aacggactgg gagaacaggt acaacatgtg ctgaatgata atcccttctc
      301 cggtcacctg tttatcttcc gtggccgacg gggggacacc gttaaaattc tttgggctga
      361 tgctgatggt ctgtgcctgt tcaccaaacg cctggaggaa ggccagttta tctggcctgc
      421 ggtgcgtgac ggtaaggtat ccattacccg ctcgcaactg gcaatgctcc tcgataagct
      481 ggactggcgt cagccaaaaa catcccgcct taacgccctg acaatgttgt aaaaaacacc
      541 tggccgcatt ataaaaacgg ccatgagtca gaaatacctc attcgcattg cagaactgga
      601 aagccagctc cgtcagaaag accagcaact gagtctggtt gaagagacgg aggccttcct
      661 gcactggcac gtgccgaaga aaagatcgaa gaagatgaac gggaaataga acatctgcgg
      721 gctcagatag aaaaactgcg ccggatgctg ttcggtaccc gttctgaaaa actgcgtcgt
      781 gaagttgaac aggctgaggc cctgctgaaa caacgcgaac aggacagcga tcgttacagt
      841 gggcgggaag acgatccaca ggttccccgc cagttgcgac agtcgcgcca tcgtcgtccg
      901 ttaccggcac accttccccg tgaaatacag cgtctggaat ctgaagaaag ctgttgcccg
      961 gagtgtggcg gtgagctgga ttatctgggg gaagtcagtg cagaacaact ggaactggtg
     1021 agcagcgccc tgaaagtgat ccgcacagaa cgggtaaaaa aagcgtgtac aaaatgtgac
     1081 tgcatcgttg aagcaccggc gccgtcccgc ccgatagagc gtggtatcgc gggccccgga
     1141 ttacttgccc gcgtgttaac gggaaaatac tgcgaacacc tgccactgta tcgtcagagt
     1201 gaaatctttg cccgacaggg tgtcgaactg agccgtgcat tactctccaa ctgggttgac
     1261 gcgtgctgcc agttaatgac gccgctgaat gatgccctgt accgttatgt gatgaatacc
     1321 cgcaagcttc acactgacga cacaccggta aaggtactgg caccgggcct gaaaaagacg
     1381 aaaacagggc gcatctggac gtatgtccgg gatgatcgca atgcgggttc gtcatctcct
     1441 ccggcggtct ggttcgcgta ctcaccgaac cggcagggga aacacccgga gcaacacctc
     1501 cgccccttcc ggggtatcct gcaggcggat gcgttcacag gttatgacag gctgttcagt
     1561 gcagaacgtg aaggtggtgc gctgacagaa gttgcgtgcc gggcccatgc ccggagaaaa
     1621 atccacgatg tatacatcag cagcaaaagt gcgacggcag aagaagccct gaagcgaatc
     1681 agtgaactgt acgccatcga ggatgaaata cggggattac cagagtcaga gcgtcttgca
     1741 gtcagacagc agcgaagcaa agcgttactg acgtcgctgc atgaatggat gatggagaag
     1801 aatggcacgc tgtcgaaaaa atccagactg ggcgaagcgt tcagctatgt actgaatcag
     1861 tgggacgccc tctgttatta cagtgatgac ggactggcgg agacggacaa taacacagcg
     1921 gaaagagcgc ttcgtgcagt ctgtcttgga aagaaaaatt acgtgttctt cggtagcgat
     1981 cacggcggcg agcgtggtgc actgctatac gggctgatcg gcacctgccg tctgaacggt
     2041 atcgatccgg aagcgtatct gcgccatatt ctgagcgtac tgccggaatg gccctccaac
     2101 cgggttgatg aactcctgcc atggaacgta gtactcacca ataaataagc gtcaatacgg
     2161 tgctccgttg acgcttacct tctttcattc gctgaaggtg gaatgtatcc acggggaacg
     2221 ctttagcagc cgggaaataa tgcgggaaac ggtgtttaat tatatcgagt gtgattacaa
     2281 tcgctggcgt cgtcacagtg cctgtggcgg tctcagcccg gactgtcttg cactgcaaat
     2341 attcagaaat gagtctatgc agttcaccag taaaatcagc catcagagag ggaatgtagt
     2401 ggcacagtaa atttggccac ctgattaaag gtgatatcct caccacaaca caaaacaggt
     2461 gacttaatga acaagaaaac caaacgtact ttcacccctg aattcaggct ggaatgtgca
     2521 cagctaattg ttgataaggg ctactcatat cgacaagcca gtgaagcgat gaatgtcggt
     2581 tctaccacgc ttgagagttg ggtgcgccag ctcaggcgag agcgtcaggg gattgcgccc
     2641 tctgccacac ctattactcc agaccagcaa cgtatccgcg aactggaaaa gcaggttcgc
     2701 cgcctggagg aacacaatac gatattaaaa aaggcttctc accgatggtc atcgcaacac
     2761 gacgccagcc aatcatggtg ccaatgccga gcgccagtgc tactgccatg atgatccaca
     2821 ccggagcgta ctcaatggtg cttaacatat cgctgcgcag ttttttcagc aggttctggt
     2881 cttctttact gacgcctggc agtttcgcca gcttcgcgga agtatcagag atgcacagca
     2941 taatgcggcg cagctggctg cgctgactca cgcttaacgg ctcgtaactt tccatattgc
     3001 ctggcagcat cgttttaacg cgcgcaatcg catcaaaggt atttcccgga tgacagtgaa
     3061 actccgtcac ttgcgcgcca tcagtagatg ccgcaggcaa tggaggttcc atcgcaatca
     3121 acttctgcgg cagttcagga tgctgttgca ggtagtgttc gaagttggta acggcatcgc
     3181 gggtacgggt aatttcatag ccggacgcgt tcatattgac gacgaagcca gcaggggcaa
     3241 tccccaccag taccagcatc accaggccga tccctttttg tccgtcgttc gcgccgtgcg
     3301 aaaacgccac gcccgcagcg gaaacaatca gcgcaatacg cgtccagaat ggcggcttac
     3361 gtttgccttt tttctttttg cgatcttccg gaatgcggtg aatacggtca cgctttttcg
     3421 ttccgctcca gtagcgtcgc agcaggaata tcaggcctcc cgcaatgacc aggccgacga
     3481 taggggaaac aatcagcgag gagaaaattt tggtcacttc acgcaggttt aacgcatcca
     3541 tcaccgatga gccggttaac agcgcgttgg ttaaaccgat gccgataatc gcgccaatca
     3601 aggtatgcga actggaggcc ggtaaaccaa agaaccacgt tcccaggttc cagataatcg
     3661 ccgccagcag catggaaaag accatcgcca ggccgtgggt tgaccccata ttcagcaaca
     3721 aatcggttgg caacatatgg acaatggcat aggcaacgct aagtccgccc aataacacgc
     3781 caaaaaagtt aaaaaatgcc gccatcacca cggcaagctg tggctgcatg gcacgagtat
     3841 aaataacggt tgccaccgca ttcgccgtgt catgaaaacc attgattgct tcgtagaaca
     3901 acacaaatgc cagagcaagt aataacaaaa gccctgtgta tatatcaagg ccaacaaata
     3961 aatttagcat atattagatt acgccatttt gaatttacga acggacgcat tatcagtgac
     4021 tttaacggtg tgggcaaagt gaaatatcat tttttaacga taaaaagagt gttatttctt
     4081 gtgtatcaga caataagcat tcattaaaaa tctttagaaa taattagaac acaggcattc
     4141 tgctggttaa tgtattcaca tatgaaatga ataaatattt gaacatataa atattgaata
     4201 ttttgattaa tccccgtact gactattctt cataatcagt acgggattgc tacaacatgt
     4261 attactcttt caccacaatt aacggttcaa tatcactctc ttttttaata accagtgatt
     4321 catcaccgcg caaacacgtc ccaccgtagt tgccgccaac ggtgaaggta catacctgaa
     4381 tgtatttacc gtccactttc ggcaaacacc acagttgctg atagatgttt ttctgctcgg
     4441 caaatttacc actggttttg tccagcacct cttcatgatg gctgacgagg tcgatgttgc
     4501 tgccacagcg accggcgatc ggtttcactg cgtaacctgt tttcaccaat tcatcattaa
     4561 cagtgaaatc ggtatccagc aggtaacgat ggtgcgggaa cagcgaccag aggatcggca
     4621 gaatcgcttt gttgccgggg atcaccgtcc acagcggctc aaagaccagc acttccgggc
     4681 gcagcaatac gtcgataaga cgcacttcat tttgcggatg accggtacgg atcggcaccg
     4741 cagcaaactc acggtcgcta acttcacgaa tctgatcaaa cgcggtttcc cacgcccagg
     4801 ttttccacac gcagttaacc agtcgccctt ccccatcaat cagttgcccg gcagcatccc
     4861 agcccagttc atccagcccg cgcaagatac gcgtttcaaa gcccgcctgg tgcagcgcct
     4921 gctccataaa ctgcgcgtga tagttttcct cgatatcttt gtcctgcatg atatggacaa
     4981 acggacgtgc acgactgtgt ttccaggcac cagccagttc gttaatcagc ccttccgccg
     5041 gattgaagcc gttgccttta tagccctgct ccgcccaacg ttcgaggatc aagcccgctt
     5101 cggtatgaca ggaggcggag tcggcgttgt actcgtaaac cttcagacca cgctcatcca
     5161 tgcagaaatc catacgaccg gtgatcatat ggtgacggcg acgctgccag gagagacgca
     5221 aacgtggcca gaggattttc ggaatgtcga acagcgccag caggttgtca tctttcagca
     5281 ccttgtcggt tgcgtgaaga tacatcaggt gcagctcgtt ggtggcttta atcagctcct
     5341 gctcggcgct ctcggtaatg gtgtagtaat gatacggatc ctgattgatc acctgaccgt
     5401 tggcctgcac ataggcgttt tgcagcggat ctttttcatc cagccattta ccgtcaaact
     5461 ggcctttgtt ttccagacgc gctccgctga ttttcagcag ctcgcctgca atttccggct
     5521 gcggtaagct gtattcagta tcttccgtct ggatcatcca gcccagaatg gtggtgtcat
     5581 caaaagtgtc tttaagggta tagcagccgt tttcgaccac catctccagt tcgcgcgtcc
     5641 actgttgccc ttgcggcaac ggggaatgaa tcacgttctg ttccgcaata cggactttgt
     5701 tgccatgtaa ttgggtaatg atggcgacat ggccagtgtc tttaaattca ccgcctttat
     5761 cccagataag aagcgcaccc gcgaccggcg cacgcggcga gccgttagga aatgcctgca
     5821 atggcaggat gttgtcatta accacttcac gcaggaagcg cagcgagaaa atctcccacg
     5881 ccatacccac gtcagtaaag accacaccgt agttcagaaa gagaaaacgg cgagcaaatt
     5941 caacgcattg ccatttgtgg cccatatatt cgtcgtcgat atagctacgg aatacggcgt
     6001 catcttcgta ttcctgcgga tcgagagaac tgtaatctga agagtagatt gctaccccac
     6061 ctggggcgta gcccaataat gtcccgaacg gggcatcctg gctggtcgtt cctttgctca
     6121 tcactttacc tttaacaata caacctaagc agttggcgta attgttgtgc tctgattacc
     6181 tgctcagcga cattaaccgg acagaggtca gactaatcat acacctcatc gcagcggctg
     6241 gcgcaaaggg ttaaaaaatt cacattctgt acagcaagtg acctgctaca ctgcttcaac
     6301 actaccactc agaaggcaac tcactatgac agacaatact tatcagcccg cgaaagtctg
     6361 gacgtgggat aaatccgctg gcggcgcgtt cgccaatatc aatcgcccgg tttctggtcc
     6421 gacgcatgaa aaaacgctgc cggtgggcaa acatccatta caactttatt cgctgggaac
     6481 gccgaacggt cagaaagtaa cgattatgct tgaggagctg ctcgcgctgg gcgttactgg
     6541 cgcagagtac gatgcctggc tgatacgcat aggcgatggt gatcaattct ccagcggctt
     6601 tgtcgaagtg aacccaaatt cgaagatccc ggcgctgcgc gatcataccc ataatccgcc
     6661 gatccgcgtg tttgagtcag gatcgattct gctttatctg gcggagaaat ttggctactt
     6721 cctgccgcag gatctggcaa aacggactga aacgatgaac tggctgttct ggttacaggg
     6781 cgcggcaccg ttcctcggcg gtggttttgg tcacttttac cattacgcgc cggtgaaaat
     6841 tgagtacgcc atcaatcgct ttaccatgga agccaagcgc ctgctcgacg tgctggataa
     6901 gcaactggcg cagcataagt ttgttgcggg cgatgagtac accattgcgg atatggcgat
     6961 ttggccgtgg tttggcaacg tggtgctcgg cggcgtgtat gatgccgcag agtttctgga
     7021 tgcgggaagt tataaacatg tgcaacgctg ggcgaaagaa gtgggcgaac gtccggcagt
     7081 gaagcgtggg cgtattgtta accgcaccaa cgggccgctg aacgagcagt tgcatgagcg
     7141 ccatgacgcc agtgattttg agacgaatac ggaagataag cgtcaggggt aaggattggt
     7201 gttcgtcgca gcaagccatc caggccggat aaggcgttca cgccgcatcc ggcaatcgag
     7261 cacaatgcct gatgcgacgc tgccgcgtct tatcagacct acgaaagcta atcatcacgc
     7321 gctggtaatg tcgtaatcca tttggcgcag tgcgtctaat gtggctttgg cttcatcttc
     7381 gtcgatgatg ctcatggcaa atccgacgtg taccagcacc cactggccca gcagatcggc
     7441 agggttacct tcacaaatca gggcgatatt cacgtcgcgc ttgataccac atacttcaac
     7501 ctgcgcaagc tggtgaatat cttcaccgac agccagcacc tggcctggaa cgccaataca
     7561 cattgttaac tccggttatt caacttcaat acttttgacg atcagcgaat cgccggtatc
     7621 gacacgcaac cgctcgccgt gacagagcgg acactgcgca tcgtgctgat gaacctccac
     7681 cacctggctg caatcccagc accaggcctg ggcgggttta tagacgatat gtaaatcgca
     7741 cccttgcgcc accgttccgt ggcagacaat ttcaaaacta aaacggacgg cgctctcctc
     7801 aacgcaggag agcgcgccaa tttccagcca cacggcggtg acgcgcttaa catcgtgctg
     7861 ctccgcctgc cgctggataa tttcaacggc gctctggcaa agcgacaact catgcatttt
     7921 cgccactcct gcgaccaaac agcagggcgc gacgccctgc gtgtggtaca tccggattcg
     7981 tgactggcag cgaaaggatc attcgtgcgc agtcatccgt caggcgttgg ccctcttcaa
     8041 tcgacatact gtgcgaaagc ggtgacatca gcgaacagga gaggtattgc gaaacaccgt
     8101 ccagttcgcc aacggtaaag gtcatggtgc catacggcag ttgcagacca attttttcac
     8161 tcactttgcg cagcggccag agttgatccg ggccggggaa gataactgca ctcagcatcc
     8221 acggggtgat cacacacccc gtccactgac cttcgaacag cgtaaaatca gaaacataca
     8281 ccggcattga aggatgcaga aaagagagag cgtgcatcga acgccgggca atttcttcaa
     8341 acgctgcctg tacttgcgcc ttcggggagg tctggaaacc tgctatctct tcagtcatga
     8401 atcgcctccc gtgggatggc ttccacgcca gattcacgca gcgcagccag aacctgctca
     8461 agcgcaggtt caatcattgc ttcaaccgtc ggcgttaagc cgatgtgtgg ctccagcgat
     8521 tccggaatta cgccgaccag ggtcagcttt ttcggaaact cgccggtgaa gcgcagggcc
     8581 gacaagacgt cggccaggcc aagctgatgc ggagagattt tgttggtaaa caacgccgga
     8641 acttcttcat cccgcaggat catcatcgtt cccggcgcgc tcttcttcga cacaatggca
     8701 tccgcaataa tcagatgatc gcgatttgcc atgtcgccaa gcagctccat tcccgccgtg
     8761 ccgccatcga ggatctcaac ataatccggc agaatgtatc gttgctctaa cgcttcgaca
     8821 atccgcacac cgatggcttc atcggtcagc aaaatattgc cgacccctaa gactaaaata
     8881 cgcattacag aaccttcact gaaaccactt cgttgccgtc agcatccact acgtgtaccg
     8941 cacaggccat gcacgggtcg aaggagtgaa tggtacgcac cacttccagc ggtttattcg
     9001 gatcagcaac cggtgtaccc accagcgact gctcgtaagg accgacgtca tcattgaagt
     9061 tacgcgggcc agagttccag gttgatggaa caactgcctg gtagttgctg atgataccgt
     9121 ctttgatcac catccagtga gagagcatac cgcgcggcgc ttcgaggaag ccaacacctt
     9181 tgaactcacc cgttgccgga atgttcggct tcacaaaggt ggtgtgatcg cctttgccga
     9241 tattggtgat cagtgcactg tattggtttt gcaggatatc ctgcaattca cagcagtgaa
     9301 cggtacgacc aataatacgg cccagcgtgg agtgcagctg tgccacttcc agcgtgttgc
     9361 cagtcagttt ctgataaatc gcaacgattt cattcagttt gttctgggta gattcgcgac
     9421 ctgccgccag tttcaccagc atattagcca gcggacccac ttctaccgtt ttgccgtaga
     9481 aagtcggtga tttcacccag gaatatttcc cgtcgtcaga ccaaccatca taagccggaa
     9541 tggtggtacc ttcccacggt gcctgcggcg cttcgtcttt ataccaggag tgcttcgcgc
     9601 tttcctgaat gcctttgatc aggtattcat cggaatgaga agtgatcgga cgatacgagg
     9661 acagatccgc attctcaatg tagccgcccg ggaacaggaa gctgccgttt ttactgtcgg
     9721 tcgggaattc cggcacgctc aggtagttca ccgcaccttt accgcgcgtc agccattccg
     9781 ggtagaacgc agcgataacc gcagtatcga ccttgtaaac ctgctcaaca aagtcactca
     9841 gcttgtcgat gaaagacttg atgtacatca ggcgctcaag gttcagcacg cccagaccgt
     9901 cgaggttgat tgggttagcg accccaccta ccgccaggtt ctgaatgtgc ggcgttttac
     9961 cgcccagcag cgccacgacg cggttagcgt cacgctggca ctccagcgcc tgcaggtagt
    10021 gcgctaccgc aatcaggttc acttccggcg gcagtttcat cgccgggtga ccccagtagc
    10081 cattggcgaa aatacccaac tgaccgctgg caaccagatc tttgatcttg ttctgaactt
    10141 tggtgaactc ttccggactg ttcaggtgcc aggtcgaaac acctttcagc atttcggagg
    10201 ctttggttgg gtcagcttgc agtgcagaag tgatgtccac ccagtccagc gccgaaagct
    10261 gatagaaatg aacgatatgg tcatgcgtgg tgtgcgcagc cagaatgatg ttacggatgt
    10321 attgcgcgtt aaccggaacg tcgatattca gcgcactttc tgccgcacga acggaagaca
    10381 gcgcgtgagt ggtagtacat acgccacaga tacgttgcac aatcatccat gcatcgcgcg
    10441 gatcgcggtt tttcacgatc tcttccatgc cgcgccacat ggtaccggaa gcccatgctt
    10501 tcgaaacgac gccattttcg atttcgcaat cgatgcgtaa atgcccctca atacgggtta
    10561 ccggatcaat agtaattctc tggctcatgc tttgctcgcc tcatgacgat tatgatcgtt
    10621 ttgttttaaa ggaggaagta tcggcagtag acgaatgagt acgatgtaag cgcaaatctc
    10681 aatagccaca aaaccaatag aaatcaacag ttcttcccag gtcgggaagt aggcgtaacc
    10741 gccgcccggg ttgaatgcca ccagcgaata ggtcagacgc caggttgcac aacctaacag
    10801 cgcgctcagt gctgacagga acagcatgcg ggaatcatta cgcagcttcg ccacacgcag
    10861 aacgaccagc gggaagagca tcagcaggac ttcaatccag aacatcacgg agtagaagtc
    10921 accggcaaac gctaacgaca gcttgtcgcg atagataagc tcgccaaagc gcagcacgat
    10981 gaaaatcgcc agcaacacac tgatggtgtt ggtcagcttg acgaataggc tcttttcatc
    11041 cggaccgttg ccacgcagac ccgcctgcac cagtgaacct tcaaagatga caatcgagaa
    11101 gcccatgatg aacgccgtca gcagcgagaa cagcggcaac atttcatagc tctgccacag
    11161 cggatgcacc ttgtagcccg ccgagatcat cagcgacccc attgaagact ggtgcatggt
    11221 cggcagcagc gcaccgagcg cgatgatgaa gaacatcacc ttgttcagac gctgtagcga
    11281 caccttccag cccagacgtt caaacagtgc cggagcaaac tccagagcca tcacgccgat
    11341 gtagatggtc atacagaccg ccgtctcgaa cagtaccgag ttcacgttga agtgacccgg
    11401 aatgtagaag tacggcaggt tccagtagcg acccacgtcg atagtgatcg acaagccacc
    11461 cagtgagtaa ccaaacagac tcgccaacag cgccggacgc accagcggat ggtattgccc
    11521 acggttaaag acgtataccg cccacgcaag cgcccagccg ccacaggcaa agccggtgcc
    11581 aatcagcagg tcaaacgcga tccacacgcc ccacgggaag ccgccgttca ggtcagagac
    11641 agagcccaga ccgaacacca gacgcttcac aatcaggagc atacagatga cgattaacgg
    11701 tccaaaaatc atgaccggtt tactgatgat tttgccgccc agcggttgtg gatcatgact
    11761 catgatcgtc tcctccgtcg tgatggtcgt ttttggtgtt gcgacgaacc agcacggtca
    11821 agcccgccag cacagccagt ggtagcatca tgcctttata cagggtgtgt tgaatatttt
    11881 cggaacgcgc accggtagaa agatcgtcca gtttcggcag gtcgagattt tcataaggca
    11941 cacccgtcag taccagaacc tgagtaccgc cgccctcttt ctcgccgtac agatgcggat
    12001 aatatttcgg caccgtatgc aggtaagtgt cgccagattt cagcgtctga cgcggatagt
    12061 ggtattcgct gccaggcttc agcgccagac gttttttcgc ctccgccatc agctcttcac
    12121 gcgtaccaaa aatcaccgcg cccgccgggc acacttctac gcagccaggc agaccgcctt
    12181 tatcgagacg ttccacacct ttctggttgc acagctcgca cttatgcagc gcaccaaacg
    12241 ggttgttgta gtcgtacttc ggcacgttgt acggacaggc gaccatgcag taacggcaac
    12301 cggtgcacac gtctttgtcg taatggacaa tgccggtttt cggatctttt ttcagtgcag
    12361 agaccgggca cacagagaca cagttcggat cgacgcagtg catacactgt ttcttaatgt
    12421 acgcgtagcc gttctcctcc tggtctttgt tgacccctgt gccgctggtc cacacttgaa
    12481 tgatgttatt ggtatacggc gacagtttgt cgttgttcga ccaggtctgt tccccttgcg
    12541 ggttacgttc agggaagttg atatcctgac acttggtgac gcaagcctgg cagcctacgc
    12601 acaaggtcga gtcgtacaac atccccagcg atcccggaat tggcgggcgg ttttcagcag
    12661 ccgcatgact gacagacggc agcgcgcccg tcagcaatgc cccgcaggag gctgctttaa
    12721 taaaattacg tctgttcacg gttattctcc ccgtgagtca gcgttatctt tcttttgctg
    12781 acgacccagt tcacgcaccg ccatcacgct gacaccggca accagcccaa ccacaccgcc
    12841 gagcaaacca atagcgcctg cagagacgtt gccgccctct ttagcgttaa catccggttt
    12901 ctgtgaacgc ggagtctgat tttcgacgtt ggcaagctga tggatgcctt tatggaagcc
    12961 gataccttct tcgttacagc catagcacgg gtgaccaatc gccaccggcc acacaccgcc
    13021 aacatcgcag aattgcagcg ttgagcagtt gccgtaagtt tctggccctt tacagccgag
    13081 gtggtacagg caccagcctt cgcggtggcc ttcatcaccg aactctttgg caaaacgacc
    13141 agcatcgaag tgcgggcgac gttcgcagtg ttcgtgaatc agacggccat aggcgaaggt
    13201 cggacggttt ttgtcatcca gtttcggcgg tttgccgtaa gtgatgatgt gcgcaacggt
    13261 cgcgaggaag ttgtgcgggt tcggcgggca gcccggaata ttgataacgg ttttgcctgg
    13321 cagaacttct tgcaagctga ctgcgccagt tgggttaact ccagctgcgg caacaccgcc
    13381 ccacgcagag caggaaccga tagcgataat ggctgctgcg ccttccgccg ctttgcggat
    13441 gtgatccaca atcggctcac cggcaaccat gcaataaata ccgttatctt ttaatgggat
    13501 ggaaccatcc accactaaca catactgccc tttgtacttc tcgagagcgt tatgtttgtt
    13561 ctcttcgacc tgatgaccga aggcggcgga aagcacttcg tgatactcca gagagatagt
    13621 ctccagtacg aggttttcta ccgttggatg cgttgcacga agcagagatt ccgtacaacc
    13681 ggtgcactcc tgcgcgccaa tccagataac tggcggacgc tgcgggttag taaccgattc
    13741 ggccatctct gcagcggctt tgctacttaa ccccatggtg gcggctaatg ctgcacaaag
    13801 cttcatgaaa tcacgacggt taatgccgtg agaatggatg agggtgttat ctccagtcat
    13861 ttatagttat tccgttgcga agacctggca tatattttgc ctcaatcgca aaatcaataa
    13921 tgcgatcgat gcgccattta ccacacattt attatggtta tcgatatcat gatactgcgg
    13981 cggggcgcta aaacgaaggg aagaagcatt agtgtaatta attaaagcaa gataatacgt
    14041 atgtttgatc aattttcgag gcgataaccg gccataaaaa agcgcaacgt agaacaggaa
    14101 ttattctcac gaaagaaatc cattaccatc tctttatcca gtccatagcg gcgcgtcaga
    14161 atacccgctt cccaggcgct aagctggcga tctccggtct tttcgtacat ccggtgtgag
    14221 tagcctaata caaaaccgcg tttataatcg gcacagaatt ttgtcacatt aaccgcactg
    14281 tcggcatgcg ttgcttttaa tcccgccatt aagcctttac caaaatggtt attcatcgtt
    14341 cacctcattc gctatatatc gtaatatata gcgatatttt aggcaaagaa cgacttttgc
    14401 gcacgctatt ttttcatgct tgtttaagac gtaatcccgt aagagataaa cctatcagga
    14461 gagatattta tcgaaccatt tcagtatcct ttgccaggca agatcggcgg cagatttgtc
    14521 ataacggggc gtggaatcat tatggaatcc gtgattaacc cccggataga tatacgcctc
    14581 ataaacctta ttattggctt tcaacgccgc ctcgtaagca ggccagccct cgttgattcg
    14641 ggtgtccagt tccgcgaagt ggagtagtaa aggcgcttca atcttcgcca catcggcagt
    14701 gggtgcctga cgaccataaa acggcactgc gcaggccagt tccgggtaag cgaccgctgc
    14761 cgcgttcgat acgccaccgc catagcaaaa tccggtaata cccactttgc ctgtcgcttg
    14821 cggatagcgt tgcataaact caattgcggc aaagaaatca ttcatcagtt tggttggatc
    14881 aacctgctgt tgcagctcac gacctttatc atcatttccc ggataacctc caacggaatt
    14941 taagccgtca ggtgccaggg cgatataccc cgccttcgcc actcgccgtg ccacatcttc
    15001 gatatacgga ttcagtccac gattctcatg caccaccacc acggctggcg ttttgccgct
    15061 catttttgcg ggtttaacca gataaccccg tacctcgccg tgaccatttg gcgaaggata
    15121 cgtgatgtac tcagcaaaaa tctccgggtc ggtaaactct acctgagtcg ccagcgcata
    15181 atttggcttg agcaaatcaa acaacgctaa tgccgtcatc ccgcctaccg catacttcgc
    15241 cgcaaggttg aggaactcac gtttcgagat tttcccgtga gcgtaatagt cgtagtaatc
    15301 cagcaactct tgtgggaaat ctttggcggt taaacgcggc atcgttgcac tcctcagttg
    15361 gtgttttttt aagcaaagca taagcacgtg tttttgccca gtttttcgtc actctgtgag
    15421 ccagactacg ggatacgcgc tggcgaatcg ctaaactaga aacattgttt cgaaattgaa
    15481 cggtggaaag gagaggtcat ggtctggtta gcgaatcccg aacgttacgg gcagatgcaa
    15541 taccgctatt gcggaaaaag cggtttacgc ctgcccgcgt tatcgctcgg tttatggcac
    15601 aatttcggtc acgttaacgc gctggaatca cagcgtgcga tcctgcgtaa agcgtttgat
    15661 ttaggcatta cgcactttga tttagccaac aattacgggc cgcctccagg aagcgcagaa
    15721 gagaactttg gtcgcctgct gcgggaggat tttgccgctt atcgcgatga actgattatc
    15781 tctaccaaag ctggctacga tatgtggccc ggcccttacg gctctggcgg ttcacgtaaa
    15841 tacctgctcg ccagcctcga ccaaagcctg aagcgtatgg ggcttgagta tgtcgatatc
    15901 ttttactctc atcgcgtcga tgaaaatacg ccgatggaag aaaccgcctc tgcgctggct
    15961 catgcggtac aaagcggtaa ggcgctgtat gtcgggatct cctcttactc accagagcgg
    16021 acgcaaaaaa tggtcgagtt gctgcacgag tggaaaattc cgctgttaat tcatcaacct
    16081 tcgtacaatt tactaaaccg ctgggtggat aaaagcggcc tgctggatac cctgcaaaat
    16141 aacggcgtgg gctgcattgc ctttactcct ctggctcagg gattgctgac cggaaaatat
    16201 ctcaacggca ttcctgaaga ttcacggatg catcgtgaag ggaataaagt tcgtggtctg
    16261 acgccgaaaa tgctcaccga agccaacctc aacagcctac gcttattgaa tgaaatggca
    16321 cagcagcgtg gacaatcaat ggcgcaaatg gcgttaagct ggttgctgaa agatgagcga
    16381 gtgacgtcgg tattggttgg tgccagccgc gcggagcaac ttgaggagaa cgtgcaggcg
    16441 ctgaataatc tgacatttag caccgaggag ctggcgcaga tcgatcagca tatcgccgat
    16501 ggcgagctga atctgtggca ggcgtcttcc gataaatgac ctgttaataa cgggcgcgag
    16561 aaccgcgccc gaatcagatc agtgattatg acgagtcagt cggtcaagat agcccattac
    16621 aaatgcagag agcacaaacg tcagatggat aatgacgtac cacatcagtt tgttatcagg
    16681 gacatttttc gcatccataa agacgcgcag taagtgaatg gaagaaattg ccacaatcga
    16741 cgctgctact ttgtttttca gcgacgttgc atccattttc cccagccagt tcagcttctc
    16801 tttgttctcg gagatatcga gttgtgagac gaaattctca taaccggaaa acatcaccat
    16861 caccagcaaa ccgccaacca gcgtcatatc caccagcgac agcaacacga ggatcaaatc
    16921 tgattccgcc atcgagaaga tattcggcag tacgtgaata atctcctgga agaacttcag
    16981 cgccagggca actaacgcca gcgaaaggcc aaagtacacg ggggcaagca gccagcgaga
    17041 agcatacatt gcattttcaa gaaaacgttc catacattcc tgtcgtgtag ttaaaacggg
    17101 caacagtata tcgcaattga gtaaacagcg cgcaacagca aatcaacata tccgttaaat
    17161 ctttgtcgca taattctgag ggttagggat cttcctggtt cccttatcaa cgcatcgaga
    17221 gcgtctaccc ttaaaggacg ttgaacctga agggagaaaa acgatgtctc atttaaaaga
    17281 cccgaccacg cagtattaca ctggtgaata tcccaaacag aaacaaccga cgccaggcat
    17341 ccaggcgaag atgacaccgg taccggattg cggcgagaaa acctatgttg gtagcggtcg
    17401 cctgaaagat cgtaaagccc tggtgacagg gggcgattcc ggaatcggtc gcgctgccgc
    17461 catcgcttac gcgcgtgaag gggctgacgt ggcgatcagt tatcttcccg tggaagaaga
    17521 agacgctcag gatgtgaaaa agatcattga agaatgcgga cgcaaagccg ttctgctgcc
    17581 aggcgattta agcgatgaga aatttgcccg ttcgctggtt cacgaagcgc ataaggcctt
    17641 aggtgggctg gatattatgg cgctggtcgc cgggaaacag gttgccattc ctgatattgc
    17701 agacctcacc agcgaacagt ttcaaaagac ctttgccatt aacgttttcg cgctgttctg
    17761 gctaacccag gaagcgatcc ccctgctacc gaaaggtgca agtattatca ccacttcgtc
    17821 aatccaggca taccagccaa gcccgcattt actggactat gcagctacga aggcggcgat
    17881 tctgaactac agccgaggcc tggcaaaaca ggtcgcggag aaaggtattc gggtgaatat
    17941 tgtcgcgcca ggcccgatct ggacagcact gcaaatttcc ggcggacaaa cgcaggataa
    18001 gatcccgcag tttggtcagc aaacgccgat gaaacgtgcg gggcaaccgg cggaactggc
    18061 ccctgtatat gtttatctgg caagtcagga gtcgagctac gtcaccgcag aagtgcacgg
    18121 cgtgtgcggc ggcgagcatt taggttaaaa aaatgcccgg ttgtgaaaag caaccgggca
    18181 tcattgtgaa ttcactctta cctgatgtgg cgaaaacgcc ttatccgcct acaaatgcat
    18241 gaagattcaa catgttgcaa tagattttgt aggcctgata agcgtagcgc atcaggcatt
    18301 gagcacctaa tgccggatgc ggcgtgaacg ccttatccgg cctacaaacg cacgaagatt
    18361 caacatgttg caatagattt tgtaggcctg atgaacgtag cgcatcaggc aattctactt
    18421 tacttcgctt tggcggtttc ttcgccgacc agacctatct tcaggtaacc cgcctgatgc
    18481 agcgtgtcca ttaccttcat caacgtctcg taatcgacag ttttatccgc tcggaagaag
    18541 atggtggtgt ctttcttgcc ttcggttaac gcattcaacg ccgtaatcat tgtttcatcg
    18601 gtgaccggat cgttaccgat aaacatcgag ttgtctgcct tcaccgacag ataaaccggt
    18661 ttttccgggc gcggctgcgg cgtgctggta gaagcaggca agttaacctt cacatctacc
    18721 gtcgctaacg gtgccgccac cataaagata atcagcagga ccaacatcac gtcgataaac
    18781 ggcgtcacgt tgatatcatg catttcgccg ttatcgtcga ggttttcgtt aagatgcatt
    18841 gccatcggac attatcctgc gcgtaatttt tgtgcgacac gaaccggatg cgcagcggcg
    18901 ctggcttcca gatccaggtc acggctttgc agcaacaata cctgcgctgc gacatcaccc
    18961 agcatcgctt taaagccgcc aatctgacgc gcgaatacgt tatagataac gaccgccgga
    19021 atcgctgcca ccagaccgat tgccgttgct aacagagctt ctgcgatacc cggcgcaacg
    19081 actgccaggt tagtggtctg cgtttgcgcg ataccaataa agctgttcat gatgccccag
    19141 accgtaccaa acagcccaac gaacggcgaa atcgcgccga tggttgccag atagccgtta
    19201 ccgcggccca tttgacgacc tactgcggcg acccgacgct ccagacggaa gctggtacgt
    19261 tctttaatac cttcgttatc gtcgctgcct tctgacagtt ccagctcatt ctgcgcttca
    19321 ttgagcaaat gcaggcttaa gcttttgcta ccaaaatcag cggcgatatc gttggcctgg
    19381 tttaaggaac gcgcttcagc cagcagttgc tgctcgcgct taaggcgacg cttctgatta
    19441 aagaactcta cgctcttact gaagaagatt gcccaggtga ctacggaggc caaaataagc
    19501 ccaatcatca cgcacttaac gacaatatcg gcgtgctgat acatacccca gacggaaagg
    19561 tccgtctgca ttaaattatt acccactgtg tatctccagg acgcaagtca caaaatctgc
    19621 gcataataat atcaaaacga cgtcgaattg atagtcgttc tcattactat ttgcatactg
    19681 ccgtaccttt gctttctttt ccttgcgttt acgcagtaaa aaagtcacca gcacgccatt
    19741 tgcgaaaatt ttctgcttta tgccaattct tcaggatgcg cccgcgaata ttcatgctag
    19801 tttagacatc cagacgtata aaaacaggaa tcccgacatg gcggacaaaa agcttgatac
    19861 tcaactggtg aatgcaggac gcagcaaaaa atacactctc ggcgcggtaa atagcgtgat
    19921 tcagcgcgct tcttcgctgg tatttgagag tatggaagcc aaaaagcacg cgacgcgcaa
    19981 tcgcgccaat ggcgagttgt tctatggacg gcgcggaacg ttaacccatt tttccttaca
    20041 acaagcgatg tgtgaactgg aaggtggcgc aggctgcgcg ctatttccct gcggggcggc
    20101 agcggttgct aattcgattc ttgcttttgt cgaacagggc gatcatgtgt tgatgaccaa
    20161 caccgcctat gaaccgagtc aggatttctg tagcaaaatc ctcagcaaac tgggcgtaac
    20221 gacatcgtgg tttgatccgc tgattggtgc cgatatcgtt aagcatctgc agccaaacac
    20281 taaaatcgtg tttctggaat cgccgggttc catcactatg gaagtccacg acgttccggc
    20341 gattgttgcc gccgtgcgca gtgtggtgcc ggatgccatc attatgatcg acaacacctg
    20401 ggcagccggt gtgctgttta aggcgctgga ttttggcatc gatgtttcta ttcaagccgc
    20461 caccaaatat ctggttgggc attcagatgc gatgattggc actgccgtgt gcaatgcccg
    20521 ttgctgggag cagctacggg aaaatgccta tctgatgggc cagatggtcg atgccgatac
    20581 cgcctatata accagccgtg gcctgcgcac attaggtgtg cgtttgcgtc aacatcatga
    20641 aagcagtctg aaagtggccg aatggctggc agaacatccg caagttgcgc gagttaacca
    20701 ccctgccctg cctggcagta aaggacacga attctggaaa cgagacttta caggcagcag
    20761 cgggctattt tcctttgtgc ttaagaaaaa actcaatgat gaagagctgg cgaactatct
    20821 ggataacttc agtttattca gcatggccta ctcgtggggc gggtatgaat cgttgatcct
    20881 ggcaaatcaa ccagaacata tcgcagccat tcgcccacaa ggcgagatcg attttagcgg
    20941 gaccttgatt cgcctgcata ttggtctgga agatgtcgac gatctgattg ccgatctgga
    21001 cgccggtttt gcgcggattg tataacattg ccacttttgg acaattttgc agacatttta
    21061 ttgtgaaaag tcttaaattg ttgcgtccgg gatcaaggcg tcccggacga ttcaggagta
    21121 caataggcag ataaaggctt aaacgctgtt ccacaggaaa gtccatggct gttattcaag
    21181 atatcatcgc tgcgctctgg caacacgact ttgccgcgct ggcggatcct catattgtta
    21241 gcgttgttta ctttgtcatg tttgccacgc tgtttttaga aaacggcctg ctgcccgcct
    21301 catttttgcc aggcgacagc ttgttgatat tggctggcgc attgattgcc cagggggtta
    21361 tggattttct gcctacgatt gcgattctga ccgccgcagc aagtctgggc tgctggctaa
    21421 gttatattca ggggcgctgg ttagggaata ccaaaacggt gaaaggctgg ctggcacagc
    21481 ttcctgctaa atatcaccag cgcgccacct gcatgtttga ccgccacggt ctgctggcgc
    21541 tgctggctgg acgttttctt gcatttgtcc gtacgctgct gccaaccatg gcgggaattt
    21601 ccggtctgcc aaaccgccgt ttccagtttt tcaactggtt aagcggattg ctgtgggtca
    21661 gcgtggtaac cagtttcggc tatgccttaa gtatgattcc gttcgttaaa cgccatgaag
    21721 atcaggtaat gacgttcctg atgatcctgc caattgcctt gttaaccgct ggcttgttag
    21781 gcacgctgtt tgtggtgatt aaaaaaaaat actgtaacgc ctgacgattt tccctgttcc
    21841 cggttgctgt accgggaacg tatttaattc ccctgcatcg cccgcattct tgccgcatct
    21901 tcccccggcg tcacaccgaa gtaacgttta aactcacggc taaattgcga tgcgctttca
    21961 tagccgacgc gcatcgctgc tgcgctggcc ttcatgccgt catggatgat catcatccgc
    22021 gccttatgca gacggtaatt cttcaaatac tgcaacggcg aggtgctggt gacagactta
    22081 aaattatggt ggaacgccga tacgctcata ttagcttctg ccgccagttg ctcgacgctc
    22141 aggttttcgg tgtatttatt ctcaatccgt ttcagcacgc ggctaatcag actgaagtga
    22201 gtctggcgac tgaccagcgc cagtaacgcg ccgccgcaag gtccggtcag cacgtagtac
    22261 agaatttcgc ggatgatctg tttgccgaga atacgcgcat ccagtggtcg cgccatcacg
    22321 tcgagtaacc gctccgccgc gcataaaatc tcttctgata acgtggcgga gttaatcccg
    22381 ctggctgcca tcgacggctg gaaatgctca tcttcgccaa tatccatcaa cagttcctgt
    22441 aactgcaaaa tatcgacatt gagacgcaac cctgccagcg gcacctctga cgtggcatag
    22501 gtttcgcact caaacggcaa cggcaccgtc agcagcaggt attcattggc atcataacga
    22561 aacacgcgtt cattgatata accgatttta tgtccggaaa agagaattat gatgccaggc
    22621 tcgtacatca ccggtgtacg tgcgaaaggc gtctcgccat acaacaaacg cacatcgggc
    22681 aacagttctg acaaactatt ttctttattt ttcagtttat taactttatc cgccagcaag
    22741 cggcaaatct cttcacgttt catatcgcgt aatttcttag gaataatgcg gcaatttgat
    22801 tgtgcgcaat tttgtagcat ttctccagca ctctggagaa ataggcaaga cattggcaga
    22861 aatgagcatt gagagccagg gcgctggcga tcacaatgaa aaacatcagg cagatcgttc
    22921 tctgccctca tattggccca gcaaagggag caagtaatga acaactttaa tctgcacacc
    22981 ccaacccgca ttctgtttgg taaaggcgca atcgctggtt tacgcgaaca aattcctcac
    23041 gatgctcgcg tattgattac ctacggtggc ggcagcgtga aaaaaaccgg cgttctcgat
    23101 caagttctgg atgccctgaa aggcatggac gtgctggaat ttggcggtat tgagccaaac
    23161 ccggcttatg aaacgctgat gaacgccgtg aaactggttc gcgaacagaa agtgactttc
    23221 ctgctggggg ttggcggcgg ttctgtactg gacggcacca aatttatcgc cgcagcggct
    23281 aactatccgg aaaatatcga tccgtggcac attctgcaaa cgggcggtaa agagattaaa
    23341 agcgccatcc cgatgggctg tgtgctgacg ctgccagcaa ccggttcaga atccaacgcc
    23401 ggtgcggtga tctcccgtaa aaccacaggc gataagcagg cgttccattc agcccatgtt
    23461 cagccggtat ttgccgtgct cgatccggtt tatacctaca ccctgccgcc gcgtcaggtg
    23521 gctaacggcg tagtggacgc ctttgtacac accgtggaac agtatgttac caaaccggtt
    23581 gatgccaaaa ttcaggaccg tttcgcagaa ggcattttgc tgacgctgat cgaagatggt
    23641 ccgaaagccc tgaaagagcc agaaaactac gatgtgcgcg ccaacgtcat gtgggcggcg
    23701 acgcaggcgc tgaacggttt gattggcgct ggcgtaccgc aggactgggc aacgcatatg
    23761 ctgggccacg aactgactgc gatgcacggt ctggatcacg cgcaaacact ggctatcgtc
    23821 ctgcctgcac tgtggaatga aaaacgcgat accaagcgcg ctaagctgct gcaatatgct
    23881 gaacgcgtct ggaacatcac tgaaggttcc gacgatgagc gtattgacgc cgcgattgcc
    23941 gcaacccgca atttctttga gcaattaggc gtgcctaccc acctctccga ctacggtctg
    24001 gacggcagct ccatcccggc tttgctgaaa aaactggaag agcacggcat gacccaactg
    24061 ggcgaaaatc atgacattac gctggatgtc agccgccgta tatacgaagc cgcccgctaa
    24121 gctttttacg cctcaaactt tcgttttcgg gcatttcgtc cagacttaag ttcacaacac
    24181 ctcaccggag cctgctccgg tgagttcata taaaggagga acgtatggct aatccaaccg
    24241 ttattaagct acaggatggc aatgtcatgc cccagttggg actgggcgtc tggcaagcaa
    24301 gtaatgagga agtaatcacc gccattcaaa aagcgttaga agtgggttat cgctcgtttg
    24361 ataccgccgc ggcctataag aatgaagaag gtgtcggcaa agccctgaaa aatgcctcag
    24421 tcaacagaga agaactgttc atcaccacta agctgtggaa cgacgaccac aatcgccccc
    24481 gcgaagccct gctcgacagc ctgaaaaaac tccagcttga ttatatcgac ctctacttaa
    24541 tgcactggcc cgttcccgct atcgaccatt atgtcgaagc atggaaaggc atgatcgaat
    24601 tgcaaaaaga gggattaatc aaaagcatcg gcgtgtgcaa cttccagatc catcacctgc
    24661 aacgcctgat tgatgaaact ggcgtgacgc ctgtgataaa ccagatcgaa cttcatccgc
    24721 tgatgcaaca acgccagcta cacgcctgga acgcgacaca caaaatccag accgaatcct
    24781 ggagcccatt agcgcaagga gggaaaggcg ttttcgatca gaaagtcatt cgcgatctgg
    24841 cagataaata cggcaaaacc ccggcgcaga ttgttatccg ctggcatctg gatagcggcc
    24901 tggtggtgat cccgaaatcg gtcacacctt cacgtattgc cgaaaacttt gatgtctggg
    24961 atttccgtct cgacaaagac gaactcggcg aaattgcaaa actcgatcag ggcaagcgtc
    25021 tcggtcccga tcctgaccag ttcggcggct aacgtgcaaa ttctcccggt ggcggtaatg
    25081 ttccgctacc ggacttttca gaaatcattt attcccctcg cgtcccgccc gttgttactc
    25141 ttccttgttc aggaatgcca aatataagga catcatcatg cagagccgga agctcttaaa
    25201 agaacaactc atctatatcc gggataaacg caacggagag gtgaaaaaca gatgaaaata
    25261 atacttctgt ttttagcagc cctggcaagt tttaccgtgc acgcacagcc cccctcacag
    25321 accgtagaac aaacagtccg gcatatttat cagaactata aatcagatgc cactgcccct
    25381 tattttggtg aaaccggaga gcgtgcgata acttctgtgc gtattcaaca ggcgcttacc
    25441 ctgaacgaca atcttacgct gccgggtaat attggctgga ttatgatccg gtttgtgatt
    25501 gtcaggattt tggcgatctg gtgctagaaa gcgtagcgat aaccgaaact gacgccgatc
    25561 atgccgatgc cgttgtgcgc tttcgtatct ttaaagatga taaagaaaag accacgcaga
    25621 cactgaaaat ggtggcggaa aatggtcgtt gggtcattga cgatattgtc agcaatcatg
    25681 gcagcgtctt acaagcagtt aatagcgaga atgaaaaaac gctggccgct ttagcttcgt
    25741 tgcaaaaaga acagccggaa gcctttgttg ccgaactctt tgaacatatt gccgattata
    25801 gctggccgtg gacgtgggtg gtttccgact cttaccgcca ggcggtcaat gccttctata
    25861 aaaccacctt caagacggcc aacaatcccg atgaagatat gcaaatagaa cggcaattta
    25921 tttacgacaa tccgatctgt tttggcgaag agtcgctatt ttcacgcgtt gatgaaattc
    25981 gagtcctgga gaaaaccgcc gattccgccc gcattcatat tcgttttacg ctgaccaatg
    26041 gcaacaacga agagcaagaa ctggttttac aacggcgcga aggtaagtgg gaaatcgctg
    26101 attttatccg cccgaacagc ggcagcctac ttaagcagat tgaggcaaaa accgccgcca
    26161 gattaaagca atgagctgaa ttaaatagca attagccgga acaataaaaa agggaacact
    26221 atatgaaaac gattttcacc gtgggagctg ttgttctggc aacctgcgtg ctcagtggct
    26281 gcgtcaatga gcaaaaggtc aatcagctgg cgagcaatgt gcaaacatta aatgccaaaa
    26341 tcgcccggct tgagcaggat atgaaagcac tacgcccaca aatctatgct gccaaatccg
    26401 aagctaacag agccaatacg cgtcttgatg cacaggacta ttttgattgc ctacgctgct
    26461 tgcgtatgta cgcagaatga taaaaaaatc cccggcagca tgtcagttgc cggggatttt
    26521 ttttaacgtc caaccgccgc tttagggcgt ttcttcgcac cagcattcac cggacgagat
    26581 tgcgtagacg acgctttttt tgccgtagca ggcgtctgac actgggtcgc catcggcgta
    26641 tgtttcgtca acgccggacg ggtattgcgg ttctggcgac gagcctcacg catctcttca
    26701 atggttggcg caggcactaa gcaatcgcga cggctgccaa tcagatgctt tttgcccatc
    26761 gcttccagcg cctggcggat taacggccag tttgccggat cgtggtaacg caacaacgct
    26821 ttatgcaaac gacgctgttt gtcgcccttc ggtacgaaga cgtcttcact cttataacca
    26881 atcttcgcca gcgggttttt cccggtgtaa tacatggtgg tcgagttagc cagtggcgat
    26941 gggtagaagt tctgtacctg gtcgagacgg aaacgatgct ttttcagcca cagcgccaaa
    27001 ttcaccatat cttcatcacg cgtaccgggg tgcgcggaga tgaaataggg gatcagatac
    27061 tgctctttac ctgcctgttt cgagtaggta tcgaacagct ctttaaagcg gtcataactg
    27121 cccatgcccg gcttcatcat cttcgacaac ggcccttctt cggtatgttc cggagcaatc
    27181 ttcagataac cgccgacgtg atgggtcgcc agttctttga tatagcgcgg atcttccacg
    27241 gcgatgtcat aacgtacgcc tgaggcaatc agaatctttt taatgccttt cagatcgcga
    27301 gcgcggcgat agaggttgat cgtcggttcg tggttagtgt ccatatgcgg acaaatatcc
    27361 gggtaaacgc aggacaaacg acggcaggtt tgttcagcgc gtggcgattt gcagcgcaac
    27421 atatacatgt tggcagttgg cccaccgaga tcggaaatta cgcccgtaaa acctggaacg
    27481 gtgtcgcgga tcgcttcgat ctcattaatg atcgaatctt cggaacggct ctgaataatg
    27541 cgcccttcgt gctcggtgat agaacagaaa gagcagccgc caaagcagcc gcgcataatg
    27601 ttgaccgaaa aacggatcat ttcgtaagcc ggaatacggg cattgccata ggccggatgt
    27661 ggcacgcgct tgtacggcag cgcaaaaacg ctgtccatct cttcggtaga aagcgggata
    27721 gcaggcgggt tgatccacac atagcggtca ccgtgttttt gcatcaatgc gcgggcgcaa
    27781 cctgggttag tttcgtggtg cagaatacgc gaagcatggg cgtacagcac tttatcgccc
    27841 ttcactttct cgaaagaagg cagcaacacg taggtttttt cccacggttt cgggcgcggt
    27901 ggctgcacgg ttacggcttt ggcttcctgc tttttcggtg ccaccggttt gttatccgcg
    27961 cacggcaaat cttcaccata cggatgcggg attgggtcga tttttccagg ggtatcaaga
    28021 cgggtggaat ccacgccgct ccagccaggc agcgcctctt tcacgataat cgcggtatta
    28081 cgcacatcgc ggatttcact aatcggctcg cccatcgcca gacggtgcgc cacctccacc
    28141 agcggacgct caccgttacc aaacatcagc atgtcggctt tcgaatccac cagcacggaa
    28201 cggcgcacgg tatcggacca gtaatcataa tgcgcggtac ggcgcagact agcctcaata
    28261 ccaccgagga tcaccggtac atctttccac gcctctttac aacgctgggt ataaaccagt
    28321 gtggcgcgat ccgggcgctt acccgcgacg ttatccggcg tgtaggcatc gtcatgacgt
    28381 aaacggcgat cggcggtata acggttgatc atcgaatcca tgttgccagc agtaacaccg
    28441 aaaaacagat tcggtttacc cagacgcatg aaatcgtctt tgctgctcca gtctggctgg
    28501 gcgatgatcc cgacgcgaaa gccctgtgct tccagcatac gaccgcaaat cgccatcccg
    28561 aagcttgggt gatcgacata cgcgtcgcca gtaaccaaaa tgatgtcgca gctatcccag
    28621 ccaagttgat ccatctcttc acgagacatc ggcaaaaacg gtgccggtcc aaaacaggcg
    28681 gcccagtact gcggccagga gaacaggtcg cgatccggtt ggatcaggga gatagagctc
    28741 attttgcttc cagaaatgat aaaaaaataa tcaaaggccg gggattataa gccggaacga
    28801 aagagaaatc gaaaagtatt ccatactcgc cctcctcggg cgagtatgaa gattacggta
    28861 ccggattgac taacagttgc ccaatcgagc cacggtccgc catttccagc gtctgactgt
    28921 tgaagtagaa cgggaagtgc gcccaggaag gctgaccgaa ataaacaagc agctccactt
    28981 gtccgtcaac ccaaacggta tctttccagc ctctgtcttc cgggaacggc atcgcaccat
    29041 tcacgttacg gatctggaac atcacgcctt caatatggaa cgcttgcggc tcgtccgcgc
    29101 gtaccgtcca gcgttcccac gttccttgct gcgcggtgac atcaatacgg ttgacgtccc
    29161 acagttgtcc attaataccc gggtcatcac ccagactgat atcgcgactg cgaatcggcg
    29221 aaccggccat gatttcagtt ggcagcaagc gcatcggaag actgtctgtg accagcggca
    29281 gaaggccggt tgggcgcagc gttagcacca gggtagaaac cagaatgctg gatggctcaa
    29341 agaagccacg aatacgatca acaatgctcg ccgcttcgcc acaggtgatc gacacttcat
    29401 caccgttgct catatccacc agaatctcgc ggcgctcgcc cggtgccaac gaaagttgct
    29461 tcaccgatac aggagcaggc aggaatccct gatcgccaga aatcacatgt aacgggcgac
    29521 catcgctcat ctgtagttga tagcgacgag agttcgacgc gttcagcagt cgcaagcgca
    29581 cccagccacg cgagacttca acgtacgggc tttgtacacc gttaaccagc agcgtatcac
    29641 caacaaagcc gccgcttccc ggttcgttgt attctggcgt accaaagtta tccagccgtt
    29701 tatcctggat aatgaccgga aaatcatcca caccataatg gttggggata ggcagcgact
    29761 tgctgacttc atcttccacc agccacattc cggcaaggcc gttatagacc tgctgagccg
    29821 tgcggttggg agtattggcg tgataccaca gagtagctgc gttctggcga ataggcagta
    29881 cgggtgccca gtcagcgttt ggcgacatca tccgtgccgg accgcccatc agcgggcctg
    29941 gtacctgtag cccggccacc gtcattgaga cattttctgt caggcggttg ctgtaaataa
    30001 gcttaacatc gtcgcccttc cagacgcgga tagtcggccc caggtaacga ccattgattc
    30061 cccagaccga cgcgcgtgtc cctggcgtaa atgaccagtg cgcacgttgt acagtcataa
    30121 acagcggttg cccacggcga gattcaagca gcggcggaac gggtagcggt tgctgttgcc
    30181 cggctgcgct ggccttcagg ggaacagcgc ctgcacaaag tgcaatcccc gatgcctgaa
    30241 tgaactgacg ccgactgagt gacatatttg ctccatgtaa aactggctaa agtaataccg
    30301 tgcaggaaag tgtttcccgc aaaaatacag attgccgact taaacttttc cggcggcttc
    30361 gcgctctgcg acttctttat cgagctcggc gattttttgt tccattatcg aacgacaatg
    30421 ggcagccagc tcacgaacct gatctttgcc atactgactg acgtcaattg gcggcagcat
    30481 ttcgacaatc accagaccgt tgtgcagtcg attaagatta atcttattcg aagttgtgga
    30541 gacgcacacg ggaataatcg ggacgcccgc cgcaattgcc gcgtgaaatg ccccagtctt
    30601 gaacggtagc aggccgcgac cacggctgcg ggtaccttcc gggaacatcc agatggaaat
    30661 acggcgtttt ttgaaatgat tcactacttc cgcaatagtg ccgtgagctt tagtgcgatt
    30721 gtttctgtcg atcaataagt tgccggttaa ccagtacaac tgcccgaaga aggggatcca
    30781 cagcaagctc tttttaccta ccgtcaccgt cggcggttgc acaatgttcg atgctgtcac
    30841 catgtcatag ttgttctggt ggttagcgat atagatagca ttgccgtagc tttcagcatc
    30901 tgcaggtttg cggcactcaa ctttcaggcc gaacagcggc gcaagacgac caaacatatg
    30961 cccaaaggtg gccacatgtt tcgggttacg cgggctgaaa aggcagtaaa tggagccgaa
    31021 tacacagact aagatgctgt aaatcacggt aataataaga cgaaagatat atagcataac
    31081 accctctgaa ggtctgagag actggcattg tacgtcacct gaaatcaggt tagggcattg
    31141 ttaaaagccg ctctctggaa aagcgtattg ttaatcgcaa cgcatgaata aacaacggtt
    31201 tcaagggaat ttttatcgtt tccgggggtt gaatacgctg ccggatgacg acttaacgtt
    31261 tcatccggcg ttccttgcaa gcgggaggaa acagcgccct ccccggcata ttactcttcg
    31321 ctatcaccgc tgctggcacg gcgaggagag tcgatctcaa cacgatcgat acgctgcaaa
    31381 ccgcgcatca acgtaccgcg gcgtccacgt tcgccagtga ctttctgtag ctcttccgga
    31441 cgcagtttaa ttttgcgttt cccaacatga atggtcagcg tgctttgcgg cggcagaacg
    31501 tacagttgcg ccagaccatc ctcgccacgc gcggcttctg ccgatggaat gttgataatc
    31561 ttgttgcctt tgcccttcga cagctgcggc agatcactta ccgggaacat caacatacgg
    31621 cctgcctgag tgattgccag cagcatatcg gaagcatctt caattaccac cggcggcata
    31681 acatgggcat tttccggtaa ggtgatcaaa gccttacctg cacggttacg cgccaccaga
    31741 tcgttaaagg tgcagacgaa accgtaaccc gcatcggaag ccatcagcag tttctgatcg
    31801 tcgctttcca tcagcatatg gtcaacggtc gccccaggcg gcaacgttaa tttgccggtg
    31861 agcggttcgc cctgaccacg cgccgacggc agcgtaatcg gatcaatggc atagctacga
    31921 ccggtggaat caacaaacac taccggttgg ttgctcttac ctttcaccgc cgctttgaag
    31981 ctatcacccg ctttataatt caggcccggc gcgtcgatat catggccttt agcgctgcgt
    32041 acccagccca tctgcgacag cacaatggtg acaggttcag acggcagcat gtcgtgctcg
    32101 ctcatcgctt tcgcttcttc gcgttcctgc aacggcgaac gacgatcgtc accgtaggct
    32161 tgcgcgtctg cctgcagttc tttcttcagc aggttattca ttttacgctc ggaagccaaa
    32221 atgccctgca actggtcgcg ctctttttcc agctcactct gctcaccgcg aatcttcatc
    32281 tcttccagtt tggcaagatg acgcagtttc agttcgagga tcgcttccgc ctgggtttcc
    32341 gtaaggccaa accgcgacat cagcgccggt ttcggttcat cttcattacg aatgatctca
    32401 atcacttcgt cgatattgag aaacgccacc agcaaacctt cgaggatatg caggcgcttg
    32461 aggactttct ccagacgata gttcagtcgg cggcgcacgg tatcgcggcg gaacaccagc
    32521 cattcggaga gaatttccag caggtttttc accgccggac gaccatccag accgatcata
    32581 ttaaggttaa tgcgatagct cttttccaga tcggtggtag cgaagaggtg gttcatcacc
    32641 tgatccatat ccacgcggtt ggaacgcggc acaatcacca gacgggtcgg gttctcgtgg
    32701 tcagattcat cgcgcagatc gtcaaccatc ggcagctttt tgttgcgcat ttgcgcagca
    32761 atttgctcca gtacgcgcgc acctgaaacc tgatgcggca atgcgctgat aaccaccgcg
    32821 ccatcttctt tcttccacac cgcgcgcata cgcactgaac cacgtccgtt ctcgtagatt
    32881 ttacggatct cggcgcgcga agtgataatt tccgcttcag tcggataatc cggcccctgc
    32941 acgatatcca gcagctgatc gagcgtggtt ttcggctggt cgattaatgc gattgccgcc
    33001 tgagccactt cacgcaggtt atgcggtgga atatcggtcg ccatgccgac ggcaataccg
    33061 gtggtgccgt taagcaaaat gtttggcaga cgggcaggta gcattttcgg ctcctgcaaa
    33121 gtgccgtcga agtttggcac ccagtcagcc gtcccctgcc ccaactcgct caatagcagc
    33181 tcggaatatt tcgacaaccg ggattcggtg taacgcattg ccgcgaacga tttcggatcg
    33241 tccggcgcgc cccagttccc ctgaccatca accagcggat aacggtaaga gaacggctgc
    33301 gccatcagga ccatcgcttc ataacaggcg ctatcgccgt gcggatggta tttacccagt
    33361 acgtcaccga cggtacgggc cgatttttta aatttggcgc tggcattcag gcccagttca
    33421 gacatcgcat acacaatgcg gcgctgaaca ggtttcagac catcaccaat aaacggcaac
    33481 gcacggtcca tgatcacgta catggagtag tttaagtagg cgttttccgt aaattcatgt
    33541 agcgcaaggc gctctgccat atcgctcatt aattctgatt cctcaactta ttcgccagcc
    33601 ttcaaagggg caatattgcc ggagatacta cattatctgc cgcgtcgagt cacaggagat
    33661 cagcgtgaat agcgccgccg gacgtgctcc gacggcgata aaggcatcaa tgtgccttga
    33721 tatacaactc tttgctgtag acataatcct ggggattatg cagcggaaaa ccgccaacgt
    33781 agggtttaag cagtttgatt aacggctgat agtagatagg aatcagcggt gcctgctggt
    33841 tgacgatcac ttctgcctgc tgatacaacg cattacgctt tgtcgcatca gtgatctgca
    33901 tggcctggtt tagtaaggcg tcatactgcg catttttcca gtgaccgacg ttttcttcac
    33961 tatcgctttt gagcgtgttc aggaagctgg aagcatcatt gtacgtcgca tcccacgact
    34021 gccgagacag catgaaatca ccggctcgtc gggcatcaag ataggttttc cactccattg
    34081 tgcgcagcgt cacctgtgca cccagccatt ttttccattc ggaagacaac gctatcgcgg
    34141 tcttttcatg cagatcgtac ttgttgtaga acagctcaaa gcgtagcgga tgagaggcgt
    34201 cgtatcccgc ctgtttcagc aaggcttttg ccatcgcgac gcgctcactc attggctttt
    34261 gcagttcatc gaacgtcgtc gcgctaaagc cttttacctc tggcggcgtc agcgtggttg
    34321 cgggcgttct caaccccagt accttttgcg caataagctg tcgatcaacc gtaagatata
    34381 gcgcccgacg cactcgcaca tcgttaaatg gcggtttctc aaggttgaag ttgtaatatt
    34441 cgctgttcag acgcggaata attcgtatct cgccaggcag tgatttttca atggcgggaa
    34501 tttgctgcgc cggaacccag gtgagatcga cctctcccgc gcgatagcgg ttatagccgg
    34561 tgaccgaatt atccagcgcc agatactcaa cctgttgcaa tactgtatgt tgcgcatcgc
    34621 ggtactttgg atttttgcgt gcagtaatct tttcgttaac tacccactga tcaagcacaa
    34681 aggcaccgtt gtaaaccatg ttctctggct tactccagct atcgccatgt ttagcgatga
    34741 catgatgagg aaccgggaac agcgtcggcc aggcgagcat cgtcgtgaac caaggaaccg
    34801 gctgctcaag cgtaacttca agagtacgat catccgtcgc tttgacaccc agcgatgtaa
    34861 catctgcttt acccgcaaca atagccgcgg cattgttaat gtgcgcctgt gccagatagc
    34921 cagcaaaagg gcttgccgtt ttcgggtcaa ccgcgcgctg ccagccgagg acaaaatcct
    34981 ctgccgtcag aggctgaccg tcggaccact gcaaaccgct acgcagatgg aaaatatagc
    35041 gcctgccgcc gtccagtatc tcccagcgtt cagcctgagc gggctgcacc tggccttcac
    35101 cgtccatcca taccagacct tcaaacagat ccagcacaat ctgcgcggca gtattctcct
    35161 ccaccttttg cgggtcgagc gtacctgggt cgctatgatt gttgtaacga aagacttgtt
    35221 gcggggcgag ctgtgtgttg gtgggaacgt cagcagcgta gagaggagcc gcacttacca
    35281 ggctgaccag ccataataaa tttcgcgtat acatggtatt ccttaccgga gaaattataa
    35341 gcccaacaac aatacgcctg tggcattgtt cgctcaaact tatcgcgagt gatttggctc
    35401 acactccggt aaagattccc catcacgcta acggcacata aacattcgtt tccagtcgct
    35461 gctccgtcac cccttcggca agattggtgt agtgaaacag aatcggtgct ttacgcattt
    35521 tctcgccgct tgcaggcagc cagtggcgaa taatgcccca tatcgtgtgt gaaatatcgt
    35581 ccagctcgcc aacgtggcga gccacggcat aacgtccacc ggtaagttca ccattgctca
    35641 caccataacg attatcggga atcggttcgc taacgctgcc gcagatatca aagcgaaacg
    35701 cttccggtgc ggtggtggca gggtcgtccc aggcgacgcc aaaagtctgg ctttggttaa
    35761 caggagaaag ccccgtctct ttacgccaca tgataaattt cgccacgctg tagttgagca
    35821 actcaggcct acagcggtgc ggcaacatcg ccacagggat tgatgggaaa tcgataatgt
    35881 ttacatccag tgtcaggttt gtcatgttgc ctccctgcga aattattccc gggaacgcat
    35941 cccgggagcg gtaacaatta cggatttact ttgcggatct gtttgacgtc aatttcaaca
    36001 gaattccagt ctttatcgac ttcaccctga atctcaaccg tatctttcgg cgtcaccgtc
    36061 acgccgttcc agcgtttgtg gtcgatatca acattgatag taccgctggc atctttgaac
    36121 acgtagagat cgtcagagat gcgttcaacg atattgccgc gcagggttac ccaggtgtcg
    36181 tcacgcaggg attttgcgct ttctacagtc gttacgctgc cgttcggccc ctggaatcct
    36241 ccggcctgac tttgcgttgc cgatgggcca gaaaaaccgc cctgctctgc tgccatcacc
    36301 ggtgcgctgc acagggccat tactgcgatt actgctgcga attttttcat gtttattact
    36361 ccctttaatg tctgtttccg agcatttaac aagatagtcc ttaacaactt cttaagggaa
    36421 aaaaataaaa tttagtgctg tacagagcgc gttacaacac ggtttactag cagcaaatac
    36481 ggttatcgca gggatgaaaa aatgcgaatt ttactgatag aagatgacat gctgattggc
    36541 gacggcatca aaacgggcct tagtaaaatg ggttttagcg tcgactggtt tacacaaggt
    36601 cgtcagggaa aagaggcgct ttatagcgca ccttatgatg cggtgatcct ggatttaacc
    36661 ttaccaggca tggatggtcg cgatattttg cgcgaatggc gagaaaaagg tcagcgtgag
    36721 ccggtactga tcctgaccgc gcgcgatgcg ctggaggaac gtgtagaagg gctgcgtctg
    36781 ggagctgacg attatctgtg taaacctttt gcgttgatag aagtcgccgc caggctggaa
    36841 gctctgatgc gccgaaccaa cggccaggcc agcaacgagc tgcgccacgg taacgtcatg
    36901 ctcgaccccg gcaaacgtat cgccacgctg gctggcgaac ccttaacact gaaaccaaaa
    36961 gaatttgccc tgctggaatt actgatgcgt aacgctggtc gggtactgcc gcgcaaactg
    37021 attgaagaga aactgtatac ctgggacgaa gaggtcacca gtaatgccgt tgaagtgcat
    37081 gtgcatcatc tgcgacgcaa actcggtagt gattttattc gtaccgtgca tggtattggc
    37141 tacacattag gtgagaaatg aaatttaccc aacgtcttag tctgcgtgtc aggctgacgc
    37201 taatcttttt aattctggcc tcggtgacct ggctgctttc cagctttgtc gcctggaaac
    37261 aaacaacgga taacgtcgat gaattgttcg acacccaact gatgctgttt gccaagcggt
    37321 taagtacgct cgatctcaac gaaatcaacg cggcggatcg catggcacag acgccaaata
    37381 aattaaaaca cggtcatgtt gatgacgatg cgctgacctt tgccatcttt acccacgacg
    37441 gcagaatggt ccttaacgat ggcgataacg gagaagatat tccctatagc tatcaacggg
    37501 aaggttttgc tgacgggcaa ctggtgggcg ataaagatca atggcgtttt gtctggatga
    37561 cctcacctga tggcaaatat cgcatcgttg ttggtcagga atgggaatac cgtgaagaca
    37621 tggcgctggc gattgttgcc gggcaattga tcccgtggtt ggtcgcactg ccggttatgt
    37681 taatcatcat gatggtacta ctgggtcgtg aactcgcgcc gctgaacaaa ctggcgctgg
    37741 cactacgtat gcgtgaccct gactcggaaa aaccactaaa cgcgactggc gtacccagcg
    37801 aagtgcgacc actggttgag tcgctaaatc aactgttcgc ccgcacacat gcgatgatgg
    37861 ttcgtgaacg acgctttacc tccgacgcag ctcacgaact tcgtagcccg ttaacggcgc
    37921 tgaaagtgca aaccgaagtt gcgcagctct ctgacgatga tccgcaggcg cggaaaaaag
    37981 cactgctcca attacattcc gggatcgatc gcgctactcg tctggttgat caactgctca
    38041 cgctatcgcg gctggactca ctggataacc ttcaggacgt cgcggagatc ccgcttgaag
    38101 atctcctgca atcgtcggtg atggatattt accacacggc gcagcaggcg aaaattgacg
    38161 tgcgactgac actcaatgtc cagggcatca aacgcaccgg acaaccgcta ttgctaagtt
    38221 tgttggtgcg aaatttgctg gataacgccg tgcgttacag tccacagggc agcgtggtgg
    38281 acgtcacgct gaatgccgat aatttcatcg taagggataa cggccccggt gtgacaccag
    38341 aggcactggc gcgaattggc gaacgcttct atcgcccacc cggacaaacc gctaccggca
    38401 gtggacttgg gctatcgatt gtccagcgaa tcgccaagct gcatggcatg aatgttgagt
    38461 ttgggaatgc ggaacaaggt ggatttgagg cgaaggtaag ctggtaaggg taagactttt
    38521 gctaaattcg caaaagactt tgcacatttt gctaatttca ccgtaccgct ctgtgacgta
    38581 ctatagtcgg caaacgtctc accttgaggt taaaaaatga gcaacatcct gattatcaac
    38641 ggcgcgaaaa aattcgccca ctccaatggt caactgaacg acaccctgac cgaagtcgcg
    38701 gatggcacac tgcgcgacct cgggcatgat gttcgcatcg ttcgcgccga cagcgactac
    38761 gatgtcaaag cggaagtaca aaactttctc tgggctgatg tggtgatctg gcagatgcca
    38821 ggctggtgga tgggcgcgcc gtggacagtg aaaaaataca ttgatgatgt attcaccgaa
    38881 ggtcacggga ctctgtatgc cagcgatggt cgtacccgca aagatccgtc gaaaaaatac
    38941 ggttccggcg gcctggtaca gggcaaaaaa tatatgcttt ctctgacctg gaacgcgcca
    39001 atggaagcct tcaccgaaaa agatcagttc ttccacggcg ttggcgttga cggtgtgtat
    39061 ctgccgttcc ataaagcaaa ccaattcctc ggtatggaac cgctgccgac atttatcgct
    39121 aatgacgtga taaaaatgcc tgatgttccc cgctatactg aagaatatcg caagcatctt
    39181 gtggaaattt ttggttaact agagctcagg ctttagaagg agttaaccat gcttaccgta
    39241 atcgcagaaa tccgtactcg tcctggtcaa catcaccgtc aggcggtatt ggatcagttt
    39301 gctaaaatcg ttccaaccgt actgaaagaa gaaggttgcc acggctatgc gccaatggtg
    39361 gattgcgctg ctggcgtgag tttccagtct atggcaccgg attctatcgt gatgattgag
    39421 cagtgggaaa gcatcgcgca tcttgaagcg catctgcaaa ccccgcacat gaaggcgtat
    39481 agcgaagccg taaaaggtga cgtgctggag atgaatattc gtattctgca gccagggatt
    39541 taacattcct gaaaagaggc cggtgttgct accggcctca ggtcagatta gccattcttt
    39601 cttcccgttt tcagcccccg atggaaatcg ttaattcgct taatggtttt aaccgcacgc
    39661 tgcggcaccg cggaagccac cgaccagaca atcatttcca ggcacaacaa taccgtacca
    39721 tgtagtggcg ttttcccctt ttcatccccg cgcggaacat gaatcacgat actggcgtct
    39781 ttgctaaaac gtgaatccag cgcgtttgtc agcaaaataa cagggatccc cagccgtctc
    39841 gcttcacgca gcgttgtcag cccttcccgg tgcgcagatt tttgcgccat catgatcaga
    39901 acatcgccac gttgaagcgc aatcagttgc tcggcaaggc caatacccgt acggtttagt
    39961 gccgtggcag gcaaacctat gcggttgaat agtcgtgcgg tatagtccgc cagaataccc
    40021 gaagcgccaa tgccaaaaat tgcgacttgc cgtgcctgta ccaacaacgc taccgcttgc
    40081 gcaagcgcgt ggcggttttc aggctctgac aggacattac aggtatacaa atgcccttca
    40141 agaacaaagt cgattgcggt gttaacatcg ctcgtcaagt tactcaccgt cgtagacatc
    40201 ttttcgctgg aactaagggc tggaccaagc cactgttcca gagtgcgttt cagatcacgc
    40261 agcccggcaa accccagggc ctgtatagcc ctgataactg tggcatcgga agtgttcaac
    40321 gtggccgcaa tttccatcgc cgtttgatcg agtaccactt cacgatgctc attaatatag
    40381 ctggcgacag ctcttaaccc aggcgttaac tgactcgcgc gagtgcgaaa acgctcacca
    40441 taaatatcaa cccgcgtttt gtctttctga atcatttggc ctccggtaaa ggtcgccccg
    40501 caatttgtga ttgtatctgc gcagccatca gccccagaga tgcaaaggag ttagaaattg
    40561 cctcttcacg agaaatcagc acataacgcc cggaacgaca ggcagtcaga aactgacacc
    40621 atcccggcat gactttttcc attgctgcca gctcatcctg aggtttacca ccggtgtcac
    40681 cgcgccaggt tgcaaaaaca aaatcggcat ccagttcagg taaacgctcc gcgctcacat
    40741 ccattcgccc accttcagga atgctctcaa tcagcggcgg aaagcgaaaa ccagcatcgc
    40801 gtaatacccg ccccagcgaa tgatagctat gcatcacatt aatttttccc tgatttgcct
    40861 gaatcaccga taccgttatt ttttggctgt ccagcgtcgc cttcagcgca ttgatttgtg
    40921 cctgatagcg gcgttcaagt atcgccagct gcgactgcgt tcctgtcagt tctgccagct
    40981 tgcgatagat ttctggcgcg ccccctttga gatgatcaat gctcaccgtg ggcgcaattt
    41041 tttccagtcg ttcaatgggt gtattacggg tcggttctgt gatgatcagg tcaggctttg
    41101 ccgccacaat cgcttcaata tcaatatcag cggtgccaat aaaagcgatc gatgaattat
    41161 caaagtcgac acctgtcaat aacgcgcctg atcgaatgaa atggctaccg tcaggccgag
    41221 ttcgcccgtg gctggcgaca ggaggtacac cgagctcaat cagaggaata gtgatatcaa
    41281 gatcgtgtaa agagacaatt ctctttggat gagcaggaac agtgactttg cgccctaaat
    41341 catcagtaaa gacctgaaca ggttctgtcg cgcgggcaaa gaatgacaac agtatcagca
    41401 gagaaaaaaa taaacgcata taactcccat taaaaactgt tcctgcgctg ccagagcagt
    41461 agcaaaaaga atggtccacc aatcaatgaa ataatgattc cggcaggcag ttgcaaaggc
    41521 aaaaaagcca aacgaccaat gctatccgcc aggatgacta acaacgcccc cgatacggcg
    41581 ctccccagca gtaatgtcgt ctgcccgcca cgtagaataa aacgtgatat atggggtgct
    41641 atcaatccga caaaaccgat gttaccaaca cacgaaacgc agacggctgt caggattatt
    41701 ggtgcggcga cacgtaacaa cgccagacga gaactattca cccctaaccc ggttgcaacc
    41761 tgatgaccaa gcagagcgat atccgccgtc ctggcggtga acagcaataa aagagcggct
    41821 ggcaacatcc aacaggctga aataccgact aacatccagt tagcagcatg caggcttccc
    41881 gataaccaca tcaatgcagt ttgtacatct cgaacatctg cagtggtcat aaatacaccg
    41941 ataccggcgg cgaaaaacca ggaaacacca ataccaatca gaacaaatcg cgggcgggaa
    42001 atttcgcgtg cacaaaaaat cacgattaaa gccaccaaca agccaccagc aagaccagcc
    42061 actggacgcc agaacattcc cagcattgga aactggaaaa tcagccacag caccgccacg
    42121 ctacatcctt ctttgacgcc gattaaacca ggatcggcaa ggccgttacg cgcaatggat
    42181 tgcatcgcgg cccccgccat acctaacatg gctccgcata gcaatgccat gatgactctc
    42241 ggcaagcgaa tatcctgcac aatataacgt gcgtctgtgc ttacgttttc tggagcaaac
    42301 aacgctcgtc ctacttccga tgccgggaca aaaaaagaac catgcatcag accgaatatc
    42361 agtattccaa ccgcaaacag aaacagccca ccaagcaaca ccagattttt agggcgtacc
    42421 agtgtcgaaa acttaccaac acgtagtgga cgtagcccga cgttcattat ccgactcatt
    42481 tgaacattct cgtcgccata atgacaaaaa caggcgcacc aactaacgcc gtcatgacac
    42541 cggtcgctaa ctcatggggc gtgaaaagcg tccgagcaat aatatctgcc agtaataaca
    42601 aaagcgcgcc gacacaagct gaaagaggga gcagaacgcg caaatccgct gataccagac
    42661 gcctgacaat ctgcggtact agcagaccga caaaaccaat ggggccagca atcgatacgg
    42721 ctgcgccaca gagcaacgcg atcgcaagca gagtgaatgt tcgcgtgcgg agtaccgaga
    42781 cgcccagccc ctgcgccatg cgatcaccca gtgccaacat gttaagagag ggcgcaaggt
    42841 aaatggctaa cacaaccccc cccagagaaa accaggcgga cgtacccagc gtcgcccaat
    42901 cctgccccgc taaatcacct gctagccatg tccgcatcgc cagcagtgtt tgttcgtcaa
    42961 gaatcaggat tgcagccgtt attgaggata cgaacgccga tagcgccacg ccgcataaag
    43021 taactttcat tggcgttagc cctgaacgcc ctgcggagga aagcagcaat atcaacagga
    43081 acaataacgc gccgcccgta gatgccagca gtgggcgtcc aaccggaaag gccagcccaa
    43141 gcgcactggc agcaacgaca gcaagagcgg ctccggcatt taagcctaaa atatgtggct
    43201 cccccaatgg gttacgaatg acagcctgca acaaagcacc agcaactcca agagaagcac
    43261 cggtaagcaa tgctgccgct aaacgcggca aacggagtct tacaattacg ttgtgatcga
    43321 agttttgcgg ttcaaaatga agaaatgcct gcaacaccgt ttgtggtgca ataaatcggg
    43381 caccaatccc tggatatacc acgcatccca caagcagtaa ggcgaggaaa attacagtaa
    43441 ccagccctgt gcgcatcata ctttttctac accacgaaac ggcatgaaga acggctttcc
    43501 cgtcagagta tttattgatg cgtgaacatc aacgtcaaag acagctttta ccagctcagg
    43561 cgtgcaatgc tccccctcat tcaacacacg caccactttc ccctggcgta aaaatatcaa
    43621 cgtatcacca taattgacgg caaaattcag atcatgcaac accacgacga ctgtacgccc
    43681 gtgatgacgc gttaaatcat gtaataactc aagaatttca acctgataac gtaaatcaag
    43741 ccaggttgtg ggttcatcca gtaggatata aggcgtcttt tgcgccaaca ccatcgcaat
    43801 ccagcaacgc tggcgctgcc caccagagag tttttcaaca ggtaaatgag caaactcctg
    43861 cgtaccggtt aatttcaggg cctcttctac cgcctgctcg tcggcgtcgc tccattgacg
    43921 tatgaaattt tgccagggaa aacgcccacg cgacaccagt tcataaacgg ttaagccctc
    43981 cggcaataat ggcgactgcg gcaagatccc aagtctacgg gataacgctt tcgttggttg
    44041 ctcgtgaatc gctttaccat ccagcaatat tgagccgccc atgggttgca agacacgtgc
    44101 aatggtgctg agcaacgtcg actttccaca gccgtttgcc ccaaccagaa cggtcatttt
    44161 ttctgtggga acggagaaag aaacaccatc aacaatgatt ttcttgtgat acccggcaga
    44221 aacattgtcg aggatcagcc cctgcccttt tgcgttatgt gccacgtgta cgccaactta
    44281 atcaaagaaa aacaaaaata aaactcattc tcttttgaga gtttgacgcc acaaaatgta
    44341 gtagtcaaga agttaaaagc catacaaaaa caaggtaaat aacaggtgaa atatttttaa
    44401 agataaaaat aaaatcatta aatattaata aattaccatc aattgaattg cattaacctc
    44461 tcataaaaac acatgtagca attttttatt atttctcatt tactactaca ttaccgcatg
    44521 attgaatgat tctcaattac atatccgatg gtaaaataac aaacggatag tgcttagtga
    44581 gcaacaagaa acaattttca ctcacgacaa tccgtgagtt tttgggataa cagactaaat
    44641 ggctaagttc acaccttcat tctcaggaat caaaggtcgg gcgctctttt cactgctctt
    44701 tgcagcacca atgattcatg caaccgacac tgcaacgacc aaagatggcg aaacaatcac
    44761 tgttacagcg gatgcaaata ccgcaactga ggcgaccgat ggttatcaac ctctgagcac
    44821 ttccacggcg acattaaccg atatgccgat gctggatatc ccgcaggtgg tcaatacggt
    44881 tagcgatcag gttctggaaa atcagaatgc aacgacgctg gatgaagcgc tttataacgt
    44941 cagtaacgtg gtacagacca atacattagg cggaactcag gacgcctttg tacgtcgtgg
    45001 ttttggtgct aaccgggatg gctccatcat gaccaacggc ctgcgaactg tacttcctcg
    45061 cagtttcaac gccgccacag aacgtgtgga agtgctaaaa ggtccggcct ccacgctgta
    45121 tggcattctc gatcctggtg gattgattaa cgtcgtgacc aagcgcccgg aaaaaacatt
    45181 ccatggttct gtctcagcca cgtcctccag ttttggcgga ggcactgggc aacttgatat
    45241 cacaggtccc attgaaggca ctcagctggc ataccgcctg acgggggaag tgcaggatga
    45301 agattactgg cgaaacttcg gtaaagagcg cagtacattt attgccccgt cactcacctg
    45361 gtttggtgat aatgcaacag taaccatgct ctattcccat cgggactata aaactccgtt
    45421 cgatcgtgga acgattttcg accttacgac gaaacagccc gtaaacgttg atcgaaaaat
    45481 acgttttgac gaaccgttta atattacaga tggtcagtcc gatctggcgc aactcaacgc
    45541 agaatatcat ctcaatagcc agtggacagc gcgctttgat tacagctaca gccaggataa
    45601 atacagcgac aatcaggctc gcgttaccgc gtatgatgca acgacaggaa cgctgacacg
    45661 gcgtgttgat gcaactcagg gatctaccca gcgtatgcat tctactcgtg cggatctgca
    45721 agggaatgtt gatattgctg ggttctataa tgagattctg ggtggggtgt catatgaata
    45781 ttatgatctt ctgcgcacag atatgattcg ctgtaaaaac gctaaagatt tcaatatcta
    45841 caaccccgtt tatggcaata ccagcaaatg cacaacggtt tcggcgtcgg acagtgatca
    45901 gacgatcaaa caggagagct actcagctta tgcacaggat gcgctctatc tgaccgataa
    45961 ctggattgcc gtcgccggga tccgctatca gtattacacg cagtatgcgg gtaaaggccg
    46021 tccttttaat gtcaatactg acagccgcga tgaacaatgg acgcccaaac tggggttagt
    46081 ctacaaactg acgccatcgg tatccttatt tgctaattat tcgcaaacat ttatgccgca
    46141 atcgtcaatt gccagctaca tcggagatct tccaccggaa tcatctaatg cttacgaagt
    46201 cggggcaaaa ttcgagctgt tcgatggtat aaccgcagat attgcgctgt ttgatatcca
    46261 taaacgtaac gtgttgtata ccgaaagtat tggtgatgaa accatcgcca aaacggcagg
    46321 ccgcgttcgt tcaagagggg tagaagtcga ccttgcggga gcattaactg aaaacattaa
    46381 tatcattgcc agctacggct ataccgatgc taaggttctg gaagatcctg attatgcagg
    46441 gaaaccattg ccgaatgttc ctcgtcatac cggttcgcta ttcctgacct atgacattca
    46501 taacatgcca ggcaataaca cactgacgtt tggcggtggt ggacatggcg taagccgtcg
    46561 ttcggcaacc aatggggctg actattatct gccaggctat ttcgttgccg atgccttcgc
    46621 cgcatacaaa atgaaattgc agtatccggt cacactgcaa ttaaacgtca aaaacctgtt
    46681 tgataaaacg tattacacct cttccatcgc cacaaataat ctggggaatc agattggcga
    46741 tccgcgtgaa gtgcaattca cggtgaaaat ggaattttga tcaaaaaagc ccggtataca
    46801 cttgtatacc gggctatgtt ttattcttct ggcgggagga ttatcagacc tcaatctccg
    46861 ccatgtcgcc tttctcttgc aaccagttgc ggcgatcttc cgagcgtttc ttcgccagca
    46921 gcatatccat catcgcgtca gtacgctgat cgtcttcatc atcgatagtc agctgcacca
    46981 gacggcgagt gttcggatca agcgtggttt cgcgcaattg catcgggttc atttccccca
    47041 gacctttaaa acgctggacg ttcggcttgc ctttcttgcg ttttaattgc tcaagtacgc
    47101 cctctttctc ttcttccgtc agcgcgtaat aaacctcttt cccgagatca atacggtaga
    47161 gcggtggcag tgcgacgtaa acgtgaccgt gtttcaccaa cgcgcggaag tgttttacga
    47221 acaaagcgca gagcagtgta gcaatgtgca aaccatcaga gtccgcatcc gcgaggatac
    47281 agattttgcc ataacgaagc tggctcagat cgtcgctgtc aggatcgata ccgatcgcta
    47341 ccgaaatatc atgcacttcc tgcgaggcca gcacttcgtc ggaagaaact tcccaggtgt
    47401 taaggatctt acctttcagc ggcatgatcg cctgatattc gcgatcgcgc gcctgcttgg
    47461 cagatccgcc tgcggagtca ccttccacga ggaacagttc ggtacggtta aggtcctgcg
    47521 cggtacaatc agccagtttg ccaggcagcg ccgggccgct ggtcagcttt ttacgcacca
    47581 cttttttagc cgcacgcata cgacgctggg cgctggaaat cgccatctct gccagcaact
    47641 ccgccgcctg aacgttctgg ttcagccaca ggatgaaggc atctttcacc acgcccgaaa
    47701 cgaatgccgc acactgacgc gaagagagac gctctttggt ctgcccggca aactgtggat
    47761 cctgcatttt tactgacagc acataggcgc agcgatccca gatatcttcc gccgacaact
    47821 ttacaccgcg cggcaaaatg ttgcggtatt cacagaactc acgcatcgcg tccagcaggc
    47881 cctgacgcag gccattaaca tgggtaccgc cctgcatcgt tgggatcagg ttgacgtagc
    47941 tttcggtcag cagttcaccg ccttccggca gccacagtag cgcccagtcc accgcttcag
    48001 tatcgccagc gaaattaccg ataaacggtt tttctggcag cgtcggtaaa ccgtttaccg
    48061 cttccgccag gtaatcattc agaccgtcct gatagcacca gcgctgttcg gtgttgttga
    48121 tctcatcttt aaaggtaatc tcaacgccgg ggcacagtac tgctttggct ttcagcacat
    48181 gcgtcaggcg tgaaacagaa aaacgcgggc tatcgaagaa ggtttcatcc ggccagaaat
    48241 ggacgctggt gccggtattg cgtttaccgc aattgccgac aacctgtaaa tcctgcacct
    48301 tttcgccatt ttcaaaggcg atgttataaa cctgaccatc gcggcgcacg ttaacttcta
    48361 cgcgcttcga cagggcgtta accaccgaaa tccccacgcc atgcaggccg ccagagaact
    48421 ggtaattttt gttagagaat ttaccgcctg catgcagacg gcaaagaatc agttcaaccg
    48481 ccggtacacc ctcttccggg tgaatatcca ccggcatccc gcgcccatcg tcaataactt
    48541 ctaacgactg gtcagcatgt aaaataacgt ccacgcgttt tgcgtgaccc gccagtgctt
    48601 catccacact gttatcaatg acttcttgcc ccaaatggtt agggcgagtg gtatcggtat
    48661 acatccccgg acggcggcga accggctcaa gcccggtgag tacctcaatg gcatcagcgt
    48721 tataagtttg cgtcatggtt taagttagca attcgagttg atcgtcagag atggtgcaga
    48781 ccaagaaaat cgacgatcgg gttgaaataa tcttcgaagc ccgtgaatgc gtggttgccg
    48841 ccttctatga cagtctggcg gcaggaagcg tagtacgcca ccgcctggcg gtaatccagc
    48901 acttcatctc ccgtctgttg cagcagccag atcaaatccg gcgcttccag cgggtcaatc
    48961 tgcatgactt taagatcgta aatatggcgt gactctagca catattgctg cccggtgtag
    49021 gggttctcgt tctgaccgag atagtccgtc agcagttcaa acgggcgcac cgccgggttt
    49081 accaccactg cgggcagcat aaaacattgt gacaaccagg tggcgtaata tccccccagt
    49141 gacgaaccga caatacccag cgaatcgccg ccatgttcca ggacaatgga ttccagcagc
    49201 tctgccgcgt cggaaggata cggcggcaac tgcggaatga tcatctcaac gtcagggtga
    49261 tgttccgcca gccagttttt taacaagctc gcttttgcag agcgcggcga gctgttgaaa
    49321 ccgtgtaaat aaagaagcgt agacatcagt agccttctga agcggtatca ggttggaaac
    49381 gtgtgtccgc caggcgatgc acctcggtgg tcagcgtgcc atcagcatgt aactcgagag
    49441 tacgccagcc gggcgcgata gtatccagcg taaagttgga acagtgcggc ttaaactgca
    49501 cacaggtcga cggtgttgcc agcaggcggc gaccgttcca gtcgagatcc agctcctgat
    49561 gaatatgacc gcacagcaag tatttgacgt gcggaaactt cgccagcacg gtatccagtt
    49621 cgcccgcgtt acgcaggctg tgttgatcaa gccaactaca acccgcaggt agcggatgat
    49681 gatgcagcag cagcaacgta tggcgctctg gcgcatcagc cagtttacgt tccagccact
    49741 caagctgaaa ctcgcttagc tcaccgtgcg gcacgccaaa cacctggcta tccagcaaca
    49801 ggatttgcca ttgctcacca ataaacaccc gcttcgccgg ggagataccc gcatcctgta
    49861 acgcgctgta catcgcgggc tggaaatcgt ggttgcccgg cagccagacg cagggcgcac
    49921 gaaaacttgc gatgccttca gcgaaatgct gataggccgc agaggattga tcctgcgcta
    49981 aatcacctgt cgcgacaatc aggtcgaatt cgtgctggtg tggacgaatc gcctccagca
    50041 ccgcctggta gctttcccag gtgtttaccc ctaagagggc ttcgtgcttt tgtgcaaaca
    50101 ggtgagtatc ggtaatttgt aaaatcctga ctctggcctc accagccaga ggaagggtta
    50161 acaggctttc caaatggtgt ccttaggttt cacgacgcta ataaaccgga atcgccatcg
    50221 ctccatgtgc taaacagtat cgcaaccagt ccgctaaaaa ctggttaatt tgatgctttt
    50281 cgtcgcgttg atgcaacttt ttattaggat aatcataccg cgctttgaag cgaaaaatct
    50341 gctggcttga acacacttca gccaccatcg cgtcatgata cagacgcacc gtcattgacg
    50401 gaaggctcca gtaactgatc gcgggcgcag tctgttctat tgtcaccagg gtagtgtatc
    50461 gggtcgattc cacaatcgtc agccgatatt gtgcgtttgc cacctgatag cttacagttt
    50521 cgccgggtgc gtcattgcgc ggtaacaaac ggcgcaattg tgaaaagttc atctcgcaca
    50581 ggcgcatcat ttcaggaaag tcaggtgtgt aacgcttcat ttatgcccac tcatttttta
    50641 acgcttgatg atgcagctgc agccattgca aagcgatgac cgacgctgcg ttgtcgattt
    50701 tcccctcttc tacccactgg tatgcctgtt cccggcttac cacatgaacg cgaatatctt
    50761 cgttttcatc agccagaccg tgaataccgc ttgcggtcgt ggcgtccact tcgcccacca
    50821 taattgacga acgctcactg gtgccccccg ggcttgccag gaaacttaac accggtttgg
    50881 tccgtttgac tatcagcccc gcctcttcaa tcgcttcgcg acgggcaaca tcttccacac
    50941 tttcaccctc ttcaatcatt ccggcaacca tctccagtag ccaaggggtt tcgctagtgt
    51001 cgtatgcggc aattcgaatc tgctcaatca gcacaacttc atcacgcact gggtcaaagg
    51061 gtagcaagac tgcggcgtga ccgcgctcaa aaatttcccg ccgtacctca tgactcattt
    51121 gcccgttgaa tagacgatga cgaaatctat aaagatctaa tgaaaaaaag ccgcgataaa
    51181 gtgtttctcg tgcaataatt tctacatcgt ttttgccaaa tgtaacgggc aggttgtctg
    51241 gcttaagcat tgttaatgtc ctggcactaa tagtgaatta aatgtgaatt tcagcgacgt
    51301 ttgactgccg tttgagcagt catgtgttaa attgaggcac attaacgccc tatggcacgt
    51361 aacgccaacc ttttgcggta gcggcttctg ctagaatccg caataatttt acagtttgat
    51421 cgcgctaaat actgcttcac cacaaggaat gcaaatgaag aaattgctcc ccattcttat
    51481 cggcctgagc ctttctgggt tcagttcgtt gagccaggcc gagaacctga tgcaagttta
    51541 tcagcaagca cgccttagta acccggaatt gcgtaagtct gccgccgatc gtgatgctgc
    51601 ctttgaaaaa attaatgaag cgcgcagtcc attactgcca cagctaggtt taggtgcaga
    51661 ttacacctat agcaacggct accgcgacgc gaacggcatc aactctaacg cgaccagtgc
    51721 gtccctgcag ttaactcaat ccatttttga tatgtcgaaa tggcgtgcgt taacgctgca
    51781 ggaaaaagca gcagggattc aggacgtcac gtatcagacc gatcagcaaa ccttgatcct
    51841 caacaccgcg accgcttatt tcaacgtgtt gaatgctatt gacgttcttt cctatacaca
    51901 ggcgcaaaaa gaagcgatct accgtcaatt agatcaaacc acccaacgtt ttaacgtggg
    51961 cctggtagcg atcaccgacg tgcagaacgc ccgcgcgcag tacgataccg tgctggcgaa
    52021 cgaagtgacc gcacgtaata accttgataa cgcggtagag cagctgcgcc agatcaccgg
    52081 taactactat ccggaactgg cggcgctgaa tgtcgaaaac tttaaaaccg acaaaccaca
    52141 gccggttaac gcgctgctga aagaagccga aaaacgcaac ctgtcgctgt tacaggcacg
    52201 cttgagccag gacctggcgc gcgagcaaat tcgccaggcg caggatggtc acttaccgac
    52261 tctggattta acggcttcta gcgggatttc tgacacctct tatagcggtt cgaaaacccg
    52321 tggtgccgct ggtacccagt atgacgatag caatatgggc cagaacaaag ttggcctgag
    52381 cttctcgctg ccgatttatc agggcggaat ggttaactcg caggtgaaac aggcacagta
    52441 caactttgtt ggtgccagcg agcaactgga aagcgcgcat cgtagcgtcg tgcagaccgt
    52501 acgttcctct ttcaacaaca ttaatgcttc tatcagtagt attaacgcct acaaacaagc
    52561 cgtagtttcc gctcaaagct cattagacgc gatggaagcg ggctactcgg tcggtacgcg
    52621 taccattgtt gatgtgttgg atgcgaccac cacgctgtac aacgccaagc aagagctggc
    52681 gaatgcgcgt tataactacc tgattaatca gctgaatatt aagtcagccc tgggtacgtt
    52741 gaacgagcag gatctgctgg cactgaacaa tgcgctgagc aaaccggttt ccactaatcc
    52801 ggaaaacgtt gccccgcaaa cgccggaaca gaatgctatt gctgatggtt atgcgcctga
    52861 tagcccggca cccgtcgttc agcaaacatc cgcacgcact accaccagta acggtcataa
    52921 ccctttccgt aactgatgac gacgacgggg cttcggcccc gtctgaacgt aaggcaacgt
    52981 aaagatacgg gttatctgcc gcattcttcc cccttctcgc ttcaatttcg accagccatc
    53041 ctctattctg atgggtattt accacaggtc ccggaagaca aaaatgaaac ggacaaaatc
    53101 catacgccac gcatcgttcc gcaaaaactg gagcgcacgc catctgacac cagtcgctct
    53161 cgcggttgcc actgttttta tgctggctgg ctgtgaaaag agtgatgaaa cagtgtctct
    53221 ctatcaaaat gctgacgact gttcagctgc aaacccaggc aaaagcgccg aatgtaccac
    53281 cgcgtacaac aatgcgctga aagaagccga acgtactgcg ccgaaatacg ccacccgtga
    53341 agactgtgtt gctgaatttg gtgaaggtca gtgccagcag gcaccagccc aggctggcat
    53401 ggcaccagaa aaccaggcgc aggcccagca atccagcggg agtttctgga tgccgctgat
    53461 ggccggttac atgatgggtc gtctgatggg cggcggtgcg ggatttgcac agcagccgct
    53521 gttctcctcg aaaaacccag ccagcccggc ttacggtaaa tataccgacg cgacgggtaa
    53581 aaactatggc gcagcccagc caggccgcac catgaccgta ccgaagacag ccatggcacc
    53641 aaaaccggcg accaccacta ccgttacccg tggcggtttt ggtgaatctg ttgccaaaca
    53701 aagcactatg cagcgtagcg caaccggtac ctcttctcgt tcaatgggtg gctgataccg
    53761 atggaaagag tcagtattac cgagcgcccg gactggcgtg agaaagccca cgaatacggt
    53821 ttcaattttc acaccatgta cggcgagccg tactggtgtg aagatgctta ctacaagttg
    53881 accctcgccc aggttgaaaa gctggaagaa gtcaccgccg aactgcacca gatgtgcctg
    53941 aaagtggtgg aaaaagtgat cgccagcgat gagctgatga ccaaattccg cattccaaaa
    54001 cacacctgga gttttgtgcg ccagtcatgg ctgacgcacc agccatcgct ttattcgcgt
    54061 cttgatctgg cgtgggatgg cactggcgaa cctaaacttc tggaaaataa cgccgatacg
    54121 ccaacgtcac tatacgaggc ggcgttcttt cagtggatct ggctggaaga tcagcttaac
    54181 gccggtaact tgccggaggg cagcgaccag tttaacagtc tgcaagaaaa actgatcgat
    54241 cgcttcgttg agctgcgtga acagtatggc ttccagttgc tgcatctcac ctgctgtcgc
    54301 gacacggtgg aagatcgcgg taccattcag tatttgcagg actgcgcgac ggaagctgaa
    54361 atcgctaccg agttcctcta cattgacgat atcgggttag gtgaaaaagg tcagttcacg
    54421 gatttacagg atcaggtaat ttccaacctg ttcaagctgt atccgtggga atttatgttg
    54481 cgtgagatgt tttccaccaa gctggaggat gcaggcgtac gctggctgga accggcgtgg
    54541 aagagcatta tctccaacaa ggcacttcta ccgctactgt gggagatgtt cccgaatcac
    54601 ccgaacctgc tgcccgctta ttttgcggaa gatgatcatc cgcaaatgga aaaatatgtg
    54661 gttaaaccga tcttctcccg tgaaggcgca aacgtgtcga tcattgagaa cggcaaaacc
    54721 attgaagcag cggaaggtcc gtatggcgaa gaagggatga ttgttcagca attccacccg
    54781 ttaccgaaat tcggcgacag ctatatgctg attggtagct ggctggtgaa cgatcaaccc
    54841 gccggaattg gcattcgtga agaccgtgca ttgatcaccc aggatatgtc tcggttttat
    54901 ccacatattt ttgttgaata atcaatgatg ccggatggca ttcgccatcc ggtaattgtt
    54961 aacctatctg caccgacagc atacttaaac tgcccatttc tataccatcg accggaatgg
    55021 taattggctc ctgcccatcc cacgcaccta agacatacag caacggcaga tagtgctctg
    55081 gcgttgggtt cgataacgcg ccaccttcat ggtcgagata attcaccaga ggatgctgct
    55141 ctaccggccc ttgccacgtc agattcgctt tcacatactc attaaacgac atcgcccaag
    55201 gatacggtga actatcaccg tgccacttca ctgtacgcag gttatgcacc acgttgccgc
    55261 tggcgaccaa cattattcct tcatcacgca gcgccgcgag ttttcgcccc atttcgaaat
    55321 gccaggcggc aggtttgcta ctgtcgatac tcaactgcac catcgggata tccgcgtcag
    55381 gatacatctt aatcagcacg ccccaggaac cgtggtcaaa gccccaggct tctttatcca
    55441 gcgtcaccgg gatcggcgct aacagctcaa ccagacgctg tgccagcgca ggcgaacccg
    55501 gagcaggata atgcgtatcg tacagcgcct gcgggaagcc accaaagtca tgaatcgtgg
    55561 gcggcgtctc catcgcggtc actcctgttc cacgggtaaa ccagtgagcc gaaactacca
    55621 caatcgcttg cgggcgtggc aatgtcatcc ccaacgtctg ccagctgcgg gtatacaaat
    55681 tatcttccag cacgttcatc ggactaccgt gacctaaaaa caatgctggc atacgtgttg
    55741 aagacatgat gatatcctta actaaaggtg tcattttgat atcctcacga tacgcttgtt
    55801 cggcggagta agaacccgga taacaatgat gatgatcatc agttattttg acgatctgcc
    55861 tgaaggtgaa gatttataag gagttgtcga tgtcagtacc tctcattctg accatactgg
    55921 cgggggcagc cacgtttatt ggcgcgtttc tcggcgttct cgggcaaaaa ccctcgaacc
    55981 gcttactggc gttttcgcta ggatttgcgg cggggatcat gttgctcatc tcattaatgg
    56041 aaatgcttcc tgccgcacta gcagctgaag gaatgtcgcc tgtgttgggt tatggaatgt
    56101 ttatcttcgg tctgcttggc tattttggcc tggaccgcat gttgccgcat gcgcatccgc
    56161 aggatttaat gcaaaaatcg gtgcagccgt tgccaaaatc gatcaagcgc acagccattc
    56221 tgctcactct cggcatcagt ctgcataact tcccggaagg gattgccacc tttgtcacgg
    56281 cgagcagcaa cctggagctg ggatttggca tcgcactggc cgtcgcgttg cacaatattc
    56341 ctgaaggtct ggcggttgca ggcccggttt atgcagcaac gggttctaaa cgcacggcaa
    56401 ttctgtgggc ggggatttct ggactggcag aaattcttgg tggtgtgctg gcgtggttaa
    56461 tcctcggtag catgatttcc ccggtggtca tggcagcaat catggcggcg gtcgcaggaa
    56521 ttatggtggc gctctcggtt gatgaattaa tgccgctcgc caaagagatt gaccctaata
    56581 ataaccccag ctatggcgta ctatgtggaa tgtcagtgat gggattcagt ttggtgctgc
    56641 tacaaacggc gggaattggt taaaagcaac atcagaagtt gtccagcgca gggacaactt
    56701 ctgctagttt cactaaagcc ttattctacc ttttcggctg tctcttctct cgtactgttt
    56761 aagtatttgt tccgcgcttc aagcagtgcg tctctgtcct ggcgaaatgc ccgacaataa
    56821 cgtttcaaat gcaggaaata acccaccaca accagaacaa tcaatacaat ccagtaccag
    56881 gcgataaaca tccttttacc cttaatattt aacaatacta aacatttagc gcataaattt
    56941 cacatatcct ttttcggata tattcatatg gtcgcataat aacggaacaa ttatatgatt
    57001 ttgatcataa tgaaaaagaa tgcctgatat taatgaaatt ttctcataga aagagaagcg
    57061 gaattaacat cttgcatagt agggaaaaat aaaaaccgaa gcctgctaca acttcggttt
    57121 cttgattaag gcagtaaatt aagcagcggt tttcagctgg ctttacgctc atgtgcctga
    57181 cggtatgcca ccaggtcttc aatagtcacg agcgccatgt tgtgtttatt ggcaaactca
    57241 atgcactctg gtgcacgcgc catcgtgcca tcgtcattag tcagctcaca cagcacacca
    57301 gccggtttga agcctgccag cgtcattaga tcaatagttg cttcagtatg accgccacgc
    57361 gtcagtacac caccagcctg agcgcggagt gggaaaacgt ggcccgggcg attcagatca
    57421 gaaggttttg cgccatcggc aatcgctgcg cgaacggtcg taatacggtc agcggcagaa
    57481 acaccggtag tcacaccttc agccgcttca atggtcacgg taaaaccggt gccataggcg
    57541 ctggtgttat tttctaccat cattggcaga tcgagttgtt tacggcgatc ttcagtaatg
    57601 cacaggcaaa caataccact accgtggcga atggtcagcg ccatctgctc aacagtcatg
    57661 gtttctgccg ggaagatcat atcaccttcg ttttcacggt cttcatcatc aagcaccatt
    57721 acaccgcgtc cttcacgcag cgcagccagt gcattttcaa cacgttcgaa aggcgtacca
    57781 aaagaggaaa gtagcgtctg attcatggta aaaaaacctc actaaaatta tggttaccag
    57841 aatcagggca gtcttaggag tggcggcata tagccaaaat aacgtgagcg ggtccatgcc
    57901 cgacagaatc gttactctct cccatccgga ctctaaccgt cggccccgga attacaccgg
    57961 atctgctgtc ctttgagttc gcacccaaag cgctcgcggg ctttcaactg agttgattta
    58021 ccgccggtgg ggaatttcgc cccgccctga gaataagcgg attcactata acgctaatga
    58081 ttagcggcag caacgcatag cttcacataa ttctggttta tgacttaccc ttatcgcact
    58141 acaatggcac tcaacaccta tcagtacagg gaaactacaa tgattgaccc gaaaaaaatt
    58201 gagcaaatcg ctcgccaggt tcacgaatca atgcctaaag gaatcaggga gttcggggaa
    58261 gatgtggaga aaaaaatccg ccaaacccta caagcgcagc tgacgcgtct cgatctggta
    58321 agccgcgaag aattcgacgt tcagacgcaa gtcctgttgc ggactcgtga aaaactggcg
    58381 ttactggagc agcgcagcag tgaactggaa gcgcgcaata attcggtagc agatcttcaa
    58441 tctcctccgg ctatcccgcc tattgataaa gcagaataaa gcaacacaaa aacgggccag
    58501 acggcccgta atatcagtgt tctaattcaa gttctctggt ttgcatcatc tgttggcaat
    58561 accaggcata acgtgcgcaa aaccaggttc tatgttcttc atccatgtta ccagtaacgg
    58621 ccagaatatc gaccgggcga ccttctgcgt gcattctggc aaaactacgc gctaggaaat
    58681 cgaaattatt taaagaatta agactatgat ccataagcac atcctccggt tatcgcatac
    58741 cttttaaggc catatgcttt ttctgatttc agtattcccc aatcagtgta
//
LOCUS       DBFIFI010000011        55504 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.011, whole genome shotgun sequence.
ACCESSION   DBFIFI010000011 DBFIFI010000000
VERSION     DBFIFI010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 55504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55504
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.011"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..599
                     /locus_tag="N7B39_000669"
     CDS             <1..599
                     /locus_tag="N7B39_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HGU2420703.1"
                     /translation="PYSIASVSDSSNTADIFPGTPFPLLFEIEVSSCTGNNTNCNSVR
                     FNFNLNTILQVNLMTCGFEDQSIDTGRFVLSRINNENYQFHHIRFDCIQGEQGELVFE
                     PSDVEYYFEPVTIQESGTSILKNELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQ
                     HPHEGSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
     gene            complement(637..1536)
                     /gene="nhaR"
                     /locus_tag="N7B39_000670"
     CDS             complement(637..1536)
                     /gene="nhaR"
                     /locus_tag="N7B39_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HGU2420704.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADV
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(1602..2768)
                     /gene="nhaA"
                     /locus_tag="N7B39_000671"
     CDS             complement(1602..2768)
                     /gene="nhaA"
                     /locus_tag="N7B39_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HGU2420705.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            3354..3506
                     /gene="mokC"
                     /locus_tag="N7B39_000672"
     CDS             3354..3506
                     /gene="mokC"
                     /locus_tag="N7B39_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HGU2420706.1"
                     /translation="MKQHKVMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(3610..4740)
                     /gene="dnaJ"
                     /locus_tag="N7B39_000673"
     CDS             complement(3610..4740)
                     /gene="dnaJ"
                     /locus_tag="N7B39_000673"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HGU2420707.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     TKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(4829..6745)
                     /gene="dnaK"
                     /locus_tag="N7B39_000674"
     CDS             complement(4829..6745)
                     /gene="dnaK"
                     /locus_tag="N7B39_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HGU2420708.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            7122..7526
                     /gene="yaaI"
                     /locus_tag="N7B39_000675"
     CDS             7122..7526
                     /gene="yaaI"
                     /locus_tag="N7B39_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HGU2420709.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNALAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            7552..8265
                     /gene="msyB"
                     /locus_tag="N7B39_000676"
     CDS             7552..8265
                     /gene="msyB"
                     /locus_tag="N7B39_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HGU2420710.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSSYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            8414..8980
                     /gene="satP"
                     /locus_tag="N7B39_000677"
     CDS             8414..8980
                     /gene="satP"
                     /locus_tag="N7B39_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HGU2420711.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(9015..9602)
                     /gene="mog"
                     /locus_tag="N7B39_000678"
     CDS             complement(9015..9602)
                     /gene="mog"
                     /locus_tag="N7B39_000678"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HGU2420712.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(9717..10670)
                     /gene="tal"
                     /locus_tag="N7B39_000679"
     CDS             complement(9717..10670)
                     /gene="tal"
                     /locus_tag="N7B39_000679"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HGU2420713.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            10949..12379
                     /gene="yaaJ"
                     /locus_tag="N7B39_000680"
     CDS             10949..12379
                     /gene="yaaJ"
                     /locus_tag="N7B39_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HGU2420714.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAIIRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVINIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVYTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSRDAWDDVSQE"
     gene            12449..13225
                     /gene="yaaA"
                     /locus_tag="N7B39_000681"
     CDS             12449..13225
                     /gene="yaaA"
                     /locus_tag="N7B39_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HGU2420715.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENTRGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(13378..13674)
                     /gene="yaaX"
                     /locus_tag="N7B39_000682"
     CDS             complement(13378..13674)
                     /gene="yaaX"
                     /locus_tag="N7B39_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HGU2420716.1"
                     /translation="MKKMQSIVLALSLVLVAPMATQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAHHDHHGGHGPGKHHR"
     gene            complement(13888..15174)
                     /gene="thrC"
                     /locus_tag="N7B39_000683"
     CDS             complement(13888..15174)
                     /gene="thrC"
                     /locus_tag="N7B39_000683"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HGU2420717.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(15175..16107)
                     /gene="thrB"
                     /locus_tag="N7B39_000684"
     CDS             complement(15175..16107)
                     /gene="thrB"
                     /locus_tag="N7B39_000684"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HGU2420718.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVESAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(16109..18571)
                     /gene="thrA"
                     /locus_tag="N7B39_000685"
     CDS             complement(16109..18571)
                     /gene="thrA"
                     /locus_tag="N7B39_000685"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HGU2420719.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(18597..18710)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(18652..18735)
                     /locus_tag="N7B39_000686"
     CDS             complement(18652..18735)
                     /locus_tag="N7B39_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HGU2420720.1"
                     /translation="MKRISTTITTTITTTITITITTGNGAG"
     gene            complement(18949..19635)
                     /gene="yjtD"
                     /locus_tag="N7B39_000687"
     CDS             complement(18949..19635)
                     /gene="yjtD"
                     /locus_tag="N7B39_000687"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HGU2420721.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLAILIQ
                     QPTKSDTTADQHQLQALRERAMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(20035..20175)
                     /gene="yjjY"
                     /locus_tag="N7B39_000688"
     CDS             complement(20035..20175)
                     /gene="yjjY"
                     /locus_tag="N7B39_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HGU2420722.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            20271..20987
                     /gene="arcA"
                     /locus_tag="N7B39_000689"
     CDS             20271..20987
                     /gene="arcA"
                     /locus_tag="N7B39_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HGU2420723.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(21047..22399)
                     /gene="creD"
                     /locus_tag="N7B39_000690"
     CDS             complement(21047..22399)
                     /gene="creD"
                     /locus_tag="N7B39_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HGU2420724.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITFGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGIGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHTGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(22457..23881)
                     /gene="creC"
                     /locus_tag="N7B39_000691"
     CDS             complement(22457..23881)
                     /gene="creC"
                     /locus_tag="N7B39_000691"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HGU2420725.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPELGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLEQALGNLLDNAIDFTPKSGRITLS
                     AEVDQEHVALKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(23881..24570)
                     /gene="creB"
                     /locus_tag="N7B39_000692"
     CDS             complement(23881..24570)
                     /gene="creB"
                     /locus_tag="N7B39_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HGU2420726.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDIVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRSL"
     gene            complement(24583..25056)
                     /gene="creA"
                     /locus_tag="N7B39_000693"
     CDS             complement(24583..25056)
                     /gene="creA"
                     /locus_tag="N7B39_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HGU2420727.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            25267..26136
                     /gene="robA"
                     /locus_tag="N7B39_000694"
     CDS             25267..26136
                     /gene="robA"
                     /locus_tag="N7B39_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HGU2420728.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(26133..26780)
                     /gene="gpmB"
                     /locus_tag="N7B39_000695"
     CDS             complement(26133..26780)
                     /gene="gpmB"
                     /locus_tag="N7B39_000695"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HGU2420729.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            26832..27344
                     /gene="yjjX"
                     /locus_tag="N7B39_000696"
     CDS             26832..27344
                     /gene="yjjX"
                     /locus_tag="N7B39_000696"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HGU2420730.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASIAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTTQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVY"
     gene            complement(27491..27817)
                     /gene="trpR"
                     /locus_tag="N7B39_000697"
     CDS             complement(27491..27817)
                     /gene="trpR"
                     /locus_tag="N7B39_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HGU2420731.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(27907..29844)
                     /gene="sltY"
                     /locus_tag="N7B39_000698"
     CDS             complement(27907..29844)
                     /gene="sltY"
                     /locus_tag="N7B39_000698"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HGU2420732.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            30055..31722
                     /gene="ettA"
                     /locus_tag="N7B39_000699"
     CDS             30055..31722
                     /gene="ettA"
                     /locus_tag="N7B39_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HGU2420733.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(31829..31996)
                     /gene="nadS"
                     /locus_tag="N7B39_000700"
     CDS             complement(31829..31996)
                     /gene="nadS"
                     /locus_tag="N7B39_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HGU2420734.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVARHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(32030..33262)
                     /gene="nadR"
                     /locus_tag="N7B39_000701"
     CDS             complement(32030..33262)
                     /gene="nadR"
                     /locus_tag="N7B39_000701"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HGU2420735.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVVDGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEEDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(33283..34665)
                     /gene="radA"
                     /locus_tag="N7B39_000702"
     CDS             complement(33283..34665)
                     /gene="radA"
                     /locus_tag="N7B39_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HGU2420736.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(34714..35682)
                     /gene="serB"
                     /locus_tag="N7B39_000703"
     CDS             complement(34714..35682)
                     /gene="serB"
                     /locus_tag="N7B39_000703"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HGU2420737.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            35788..36429
                     /gene="ytjB"
                     /locus_tag="N7B39_000704"
                     /pseudo
     CDS             35788..36429
                     /gene="ytjB"
                     /locus_tag="N7B39_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
     gene            36460..37476
                     /gene="lplA"
                     /locus_tag="N7B39_000705"
     CDS             36460..37476
                     /gene="lplA"
                     /locus_tag="N7B39_000705"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HGU2420738.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGIPHEQVCEAITEAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            37508..37771
                     /locus_tag="N7B39_000706"
     CDS             37508..37771
                     /locus_tag="N7B39_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU2420739.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(37932..38651)
                     /gene="deoD"
                     /locus_tag="N7B39_000707"
     CDS             complement(37932..38651)
                     /gene="deoD"
                     /locus_tag="N7B39_000707"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HGU2420740.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(38708..39931)
                     /gene="deoB"
                     /locus_tag="N7B39_000708"
     CDS             complement(38708..39931)
                     /gene="deoB"
                     /locus_tag="N7B39_000708"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HGU2420741.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(39983..41305)
                     /gene="deoA"
                     /locus_tag="N7B39_000709"
     CDS             complement(39983..41305)
                     /gene="deoA"
                     /locus_tag="N7B39_000709"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HGU2420742.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(41432..42211)
                     /gene="deoC"
                     /locus_tag="N7B39_000710"
     CDS             complement(41432..42211)
                     /gene="deoC"
                     /locus_tag="N7B39_000710"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HGU2420743.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            42469..44018
                     /gene="yjjI"
                     /locus_tag="N7B39_000711"
                     /pseudo
     CDS             42469..44018
                     /gene="yjjI"
                     /locus_tag="N7B39_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
     gene            43990..44853
                     /gene="yjjW"
                     /locus_tag="N7B39_000712"
     CDS             43990..44853
                     /gene="yjjW"
                     /locus_tag="N7B39_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HGU2420744.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQYPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAAFIKGVGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALRERG
                     VSRLIFPALYL"
     gene            complement(45067..45849)
                     /gene="yjjV"
                     /locus_tag="N7B39_000713"
     CDS             complement(45067..45849)
                     /gene="yjjV"
                     /locus_tag="N7B39_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HGU2420745.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGESGLDL
                     FGDDPQFERQQWFLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLSRTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRVVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFDVLCELRPEPEDEIAEVLLNNTYAVFNVRG"
     gene            complement(45846..46919)
                     /gene="yjjU"
                     /locus_tag="N7B39_000714"
     CDS             complement(45846..46919)
                     /gene="yjjU"
                     /locus_tag="N7B39_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HGU2420746.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAYICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(47041..47202)
                     /gene="ytjA"
                     /locus_tag="N7B39_000715"
     CDS             complement(47041..47202)
                     /gene="ytjA"
                     /locus_tag="N7B39_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HGU2420747.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(47329..47934)
                     /gene="osmY"
                     /locus_tag="N7B39_000716"
     CDS             complement(47329..47934)
                     /gene="osmY"
                     /locus_tag="N7B39_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HGU2420748.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGIVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(48327..49916)
                     /gene="prfC"
                     /locus_tag="N7B39_000717"
     CDS             complement(48327..49916)
                     /gene="prfC"
                     /locus_tag="N7B39_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HGU2420749.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(50007..50684)
                     /gene="yjjG"
                     /locus_tag="N7B39_000718"
     CDS             complement(50007..50684)
                     /gene="yjjG"
                     /locus_tag="N7B39_000718"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HGU2420750.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLNVEPGKLNEAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHHREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(50699..51145)
                     /gene="rimI"
                     /locus_tag="N7B39_000719"
     CDS             complement(50699..51145)
                     /gene="rimI"
                     /locus_tag="N7B39_000719"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HGU2420751.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(51114..51527)
                     /gene="holD"
                     /locus_tag="N7B39_000720"
     CDS             complement(51114..51527)
                     /gene="holD"
                     /locus_tag="N7B39_000720"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HGU2420752.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            51630..52661
                     /gene="rsmC"
                     /locus_tag="N7B39_000721"
     CDS             51630..52661
                     /gene="rsmC"
                     /locus_tag="N7B39_000721"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HGU2420753.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFQHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            52851..52937
                     /locus_tag="N7B39_000722"
     tRNA            52851..52937
                     /locus_tag="N7B39_000722"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:52885..52887,aa:Leu,seq:cag)
     gene            complement(52976..53212)
                     /gene="yjjZ"
                     /locus_tag="N7B39_000723"
     CDS             complement(52976..53212)
                     /gene="yjjZ"
                     /locus_tag="N7B39_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HGU2420754.1"
                     /translation="MLQRTLGSGWGVLLPGFLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
     gene            53353..54141
                     /gene="fhuF"
                     /locus_tag="N7B39_000724"
     CDS             53353..54141
                     /gene="fhuF"
                     /locus_tag="N7B39_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HGU2420755.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDARLAQAVRATIAEHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIREYKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTTGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(54179..54856)
                     /gene="bglJ"
                     /locus_tag="N7B39_000725"
     CDS             complement(54179..54856)
                     /gene="bglJ"
                     /locus_tag="N7B39_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HGU2420756.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCET
                     SNVLHPY"
     gene            complement(54814..>55504)
                     /gene="yjjQ"
                     /locus_tag="N7B39_000726"
     CDS             complement(54814..>55504)
                     /gene="yjjQ"
                     /locus_tag="N7B39_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU2420757.1"
                     /translation="SKIPVMQAGLKEVMRTHFPEYEIISSASAEDLTLLQLRRSGLVI
                     ADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLMCPTATLLSDVEP
                     IENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERKVLRLLGKGWGIN
                     QIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELNLPSVYGDAAEWN
                     TAELRREMSHS"
BASE COUNT        13279 a        14929 c        13846 g        13450 t
ORIGIN      
        1 ttccatactc tatcgcatct gtatcagata gttccaacac cgcagatatt tttccaggaa
       61 ctccttttcc tttgttgttc gaaattgaag tgagttcatg tacagggaat aatactaatt
      121 gtaatagcgt ccgctttaat tttaatttaa atactatttt acaggtaaat ttaatgacct
      181 gtggttttga agatcaatcc atcgacactg gacgatttgt actgtctcgt atcaataatg
      241 aaaattatca gtttcatcat ataagatttg actgcatcca gggcgagcaa ggtgaattag
      301 tttttgaacc cagtgacgtt gaatattact ttgaaccagt gacgatacag gagtcaggaa
      361 ccagcatatt aaaaaatgag ttagaaaaca gtgaggacgg tgcgggtcaa ataggctttc
      421 aactctccaa tgatggtacg aatgaaatcc agtatggtaa aagcaattac tactcttttc
      481 agcatcccca tgaaggaagt aatcagattc ctctatttat tcgcccgcgt acatatggaa
      541 acaatgtctc aagcgggcaa ataatgagcc gagtcaaaat cgtggtaatg tacaactaat
      601 ctatctcccc caccttaact ttgggggagc tgccgattaa cgcaccgctg gactaaaaag
      661 cgcagaataa tccgtattgc agattcgctg taccgccggg tgctgaatca tccgctcagc
      721 aaaaatagca tggtactctt ccatcacatt ctcgacgcga ccaatttcta cgacagtttt
      781 atcggcataa aagtcatatg cataaagcgt tggggcaacg aagattgcat tgtgcatcgc
      841 accaaaagct ttcatcaaag cggcatcatc aaactcgcca aggatttcta cgtttaatcc
      901 ctgggagtta aaccagttaa gtaatttgcg ccctaacatt gaacgtcgcc caggaatcaa
      961 aagccgccgt tcttcaagac aagctgggaa cggtttttcc ggcggtggat ttgtacacca
     1021 gaaactcacg ccacattcgc caattctcac ggagaacagg ccttcctgct gcgtagaatc
     1081 tatcggacag tcggaaatga tcatatccag tttatgctgg cttaattgct ccagcagcat
     1141 ttcgtgggtg gattcgaagc agcgaagatg aatcggctcg ccttctacta ctgcagcatt
     1201 cagcacgcta ctgaccagac gtttggaaag tacatcagcc acgccaacgt caaacaataa
     1261 attggattct ttgcgatagt tcacgatatc cagcatttcc tggctcaagg taaacatttt
     1321 atcggcatag cgatagacca gctcccccag ctcgctgggc tcgagaccac gtcccttgcg
     1381 cttaaataat ttgccttgca ggcgctcttc cagcgcccga atctgtccgg taatggtttg
     1441 tggtgttaaa taaagcgcct ccgctgcgcc aaccacagaa ccttctttat aaacatgcca
     1501 gaagtaatac aagtggttgt aattgatatg agacatgctc atttctctcc ctgataacaa
     1561 tgaaaaggga gccgtttatg gctccccggt aaaccgtcct gtcaaactga tggacgcaaa
     1621 cgaacgcgta accagctgta tccaattacc gccgaagaga tagaaccgac caggataccg
     1681 agtttcgccc agttaatcag ttctggatct acgctaccaa aggccaggct ggcaataaag
     1741 atagacatag taaaaccgat accgcacagg atccccaccg ccataatttg ctgataagtc
     1801 gttccctcag gcagatgcgc cagtttcaaa cgcagcgcca accagcagaa cagactaatc
     1861 cccagcggtt tgccaatcag caagccagcg atgatcccca atggcagaat ggaggtcaag
     1921 ccatccagcg tgacgccttg cagtgaaacg ccagcattag caaatgcaaa cagtggtaaa
     1981 atcagatacg ccacccacgg gtgcaacaca tgctccagtc gcttcgctgg agaacgccca
     2041 tgcttctctt tcaaaggaat aaagaagccg acaattaccc ccgccagggt tgcgtgaacc
     2101 cccgatttca acactgcagt ccacaacact acgccaacca gaatatagac gcccgtgcgg
     2161 cgtacaccac acagattcaa taccgcgagt acagcaattg ctacagccgc gacgccaaga
     2221 gaggccatcg ataagtcatt agtgtagaac aatgcgatga taatgatggc cccaagatcg
     2281 tcgataatag ccagagccat caaaaagatc ttcagcgcta acggaacacg acttcccaat
     2341 agcgccagca caccaagtgc aaaggcaatg tcagtagccg ccgggatcgc ccacccttcg
     2401 cgggtaatcg gatcggcata gttaaaggcc agatagagta atgccggcac aatcatccca
     2461 ccaatagcag cgataactgg aaatgcggcc tggcgtaagc tggctagcga tccttgcatc
     2521 agttcacgtt taacttccag accgactaac aggaaaaata ccgccatcag cgcgtcattt
     2581 atccataaca gcatgttttt gttgatttcg agtgaaccaa cccggagctg aaccggcgtc
     2641 tccagaaagt cgtgatacca tccactggtt gcgccgctgt tggccatcat catcgccagg
     2701 atagcggcaa tgataagaat aatgcctccc gaggcatcac tgctaaagaa tcgatgcaga
     2761 tgtttcactt tttatttctc tttcaggtga atagatcgtt tttactattt taccccgcac
     2821 gaatcatcgt taaaatcaga ttatagtgta ggaataattc gttttttacg atctatagaa
     2881 gaatttgcgc catcagcggt gagatgcgtt tttgacggct gtaagcgctg acgggtgacc
     2941 ctaagccctg ataatcatag cttaaggtga ggatttctga gggattaatt aggaaaagta
     3001 ataattaatt tgttgtcagt cggcgatgtt tgttaggcca atatttcaaa aataatccag
     3061 cttgtcgtca ataattgcga tgaagagata aattaaagtt aaataattaa atcattaaaa
     3121 aattacgcca taaaaatgcg aggcgtcttc cggattagaa tcgataaaac agaatgggtt
     3181 aaagagagcg atataatagc ggcgggtgct tgaggctgtc tgtctcaggc attagctgaa
     3241 cggcagatag agaaaagccc cgagtgatat tttaccatca acccgaggcc ccctatatgc
     3301 tgaacacatg tagagtgcct cttactgacc gtaaggtcaa ggagaagaga gcaatgaagc
     3361 agcataaggt gatgattgtc gccctgatcg tcatctgtat caccgccgta gtggcggcgc
     3421 tggtaacgag aaaagacctc tgtgaggttc acatccgaac tggccagacg gaggttgctg
     3481 ttttcacggc ttacgaatcc gagtaagagc aacggcgggg agtgatcccc gccactttac
     3541 aggtgctcgc atatcttcaa cgcaccctat ttttacccag gcctgcccac gggcaggctt
     3601 ttggggaggt tagcgggtca ggtcgtcaaa aaacttcttc acaccatcaa agaagctctt
     3661 tgagcgcggg ctgttgtgct cgccggttgg gccaccgaag ctttcttgca gctcttgcag
     3721 cagctgtttc tgcttctcgt tcaggcctac cggtgtttcg acgacaacgc ggcacagcaa
     3781 atcaccctgt gcgccaccgc ggacagactt gacgccttta ccgcgcatac ggaatagctt
     3841 accggtctgg gtttcgccag gcactttcag tttgacgcga ccatcaaggg tcggtacttc
     3901 gatttcacca cccagcgccg ccatagcgaa gttgatcggg acttcgcaat acaggttgtt
     3961 gccttcacgc tcgaaaatcg ggtgctgttt aacctgaacc tgaacgtaca gatcgcctgc
     4021 cggtgcgccg tgttcacccg cttcaccttc gcccgcaaga cggatgcggt ctccagtgtc
     4081 cacccctgcc gggattttaa cggacagcgt tttgctgcgc tcaacacgac catgaccatg
     4141 acatttgttg cacggatctt tgatcagcgt accgcggccc tgacagtgtg gacaggtctg
     4201 ctgcacggca aagaaaccct ggcgcatctg cacctgacca gaaccatgac aggtcggaca
     4261 ggtctgcggc tgtgtacctg gttttgcacc gctaccgtgg caaacgtcac actcttccag
     4321 agtcggaatg cggatctctt tggtcacgcc acgtacagct tcttcgaggg tgagctccat
     4381 gttatagcgt aaatcagcac cgcgcgccgc acgttgacga ccacgtccgc cgccaaaaat
     4441 atcgccgaaa acgtcaccaa aaatatcgct gaagtctgcg ccgccgccaa aaccgccgcc
     4501 gcccatgcca ccttgctcaa acgcagcatg accatactga tcgtatgccg cacgtttttg
     4561 cgagtcggtc agaacttcat aagcttcctt gatctcttta aatttcgtct cggcctcttt
     4621 gtcaccctgg ttacggtccg ggtggtattt catggccagg cgtttgtagg cctttttgat
     4681 ttcacgctct tccgctgttt tggaaacgcc taaaatctcg taataatctt gcttagccat
     4741 cttttttaaa ttgcccctag atgaatgcac gggcggagag gaaattcccc ttcgcccgtg
     4801 tcagtataat aacccgttta tagggcgatt attttttgtc tttgacttct tcaaattcag
     4861 cgtcgacaac atcgtcatct ttcgcgttgt ttgcagaagc atcagcaccg gcagtctgct
     4921 gctgggcatg ttgctgctgg gcgatttcca tcagtttctg ggaaacctgt gccagttcct
     4981 gcattttcgc ttcgatagcg gctttgtctt cacctttcag agcagtttcc agtgcagtca
     5041 gcgcagactc gatagcagtt ttgtcgtcag ccggcagttt gtcgcctgct tcttcaacct
     5101 gcttacgggt gctgtgcagc agatggtcgc cctggttgcg agtctgtacc agctcttcaa
     5161 acttacggtc agcttcggcg ttagcttcgg cgtcgcgtac cattttctgg atttcatctt
     5221 cgttcagacc agaagacgcc ttgatggtga tcttctgctc tttaccgctg tttttatctt
     5281 tcgcggaaac gtgcaggata ccgtcagcat cgatatcgaa ggtaacttcg atctgcggca
     5341 tgccgcgcgg tgccgggttg ataccatcca ggttgaactg acccagagat ttgttgtcag
     5401 ccgcacgttt acgttcaccc tgcagcacat ggatggttac cgcagactgg ttgtcttcag
     5461 cggtagagaa cacctggctg tgcttggtcg ggatagtggt gtttttcgcg atcagcgtcg
     5521 tcatcacacc gcccatggtt tcgataccca gagacagcgg ggtaacatcc agcagcagta
     5581 cgtcttttac gtcaccagtc agaacaccac cctgaacagc agcaccgatt gctacagctt
     5641 cgtccgggtt aacgtcttta cgcggctctt taccaaagaa ctcagcaact ttcttctgaa
     5701 ccattggcat acgagtctga ccaccaacga ggataacgtc gtcgatatca gatacggaca
     5761 ggccagcgtc ctgcagcgca actttcagcg gctcgatgga acggttaacc agatcttcaa
     5821 ccaggctttc cagtttcgca cgagtcactt tgatgttcat gtgtttcgga ccggtcgcgt
     5881 ctgcagtgat gtacggcagg ttaacgtcgg tctgctgagc ggaagacagt tcgattttcg
     5941 ctttttctgc cgcttctttc aggcgctgca ttgccagcgg atcgttgcgc aggtcaatgc
     6001 cctgatcttt cttgaattct tcaaccagat agttgatcag acggctgtcg aagtcttcac
     6061 cacccaggtg ggtatcaccg ttggttgcca gaacttcgaa ggttttttcg ccgtcaactt
     6121 cgtcgatttc gataatagaa atatcgaaag taccaccacc caagtcataa accgcgatag
     6181 tacggttgcc agtacctttg tccagaccgt aagccagcgc agctgcggtc ggttcgttga
     6241 tgatacgttt tacttccaga ccagcgatac ggcctgcgtc tttggttgcc tgacgctgag
     6301 catcgttaaa gtatgccggt acggtgataa cagcttcagt taccggttca cccaggtaat
     6361 cttcagcggt tttcttcatt tttttcagca cttcagcaga aatctgcggc ggtgccattt
     6421 tctggccttt aacttcgacc catgcgtcgc cgttatcagc agcaataatt ttgaacggca
     6481 tgatggaaac atcacgctgt acttcttcgt cctggaagcg gcggccaatc aggcgtttaa
     6541 tcgcaaacag ggtgttttgc gggttcgtca ctgcctgacg tttagccggc tgaccaacca
     6601 gagtttcacc atcctgggta taggcaatga tagaaggcgt ggtgcgatcg ccttcggcgt
     6661 tctccagcac acgaggagtg gtgccatcca taatcgctac acaagagttg gtagtaccca
     6721 ggtcgatacc aattatttta cccatctaaa cgtctccact atatattcgg tcatcatgtg
     6781 gttgtgagtc tgtaataggg gcgaaacgtc tggtttcaac tgcccaattt catttttttg
     6841 cagactcact cactgcggtt gactactaaa tggggtcgta acccacgtca tcaaggggga
     6901 gatgcaaaaa ttttttgtgc ttttgcgcag aaatttcgcg atatggcagg acaagacgta
     6961 aaattgacag caattttatt catataaaga atgaatcgca gtcaaggtat tcatcaaaaa
     7021 attgtaataa aaagaaaaga ttacgtgcct gaatcttctc tttatcagca gtaaactagt
     7081 gggtattcat ccccctacct cttcccacta agagaatcct tatgaaatcc gtttttacga
     7141 tttccgccag cctggcgatt agcctgatgc tgtgctgcac ggcgcaggca aacgaccata
     7201 aactcctcgg cgtcattgca atgccgcgta acgaaaccaa cgctctggcg ctgaaacttc
     7261 ctgtttgtcg cattgtgaaa cgcatacaac tctctgccga ccatggcgat ttacagttaa
     7321 gcggtgcatc ggtttatttc aaagccgccc gtagcgccag tcagagcctg aatattcctt
     7381 cagaaataaa agaagggcaa accactgact ggatcaacat taacagcgat aacgacaata
     7441 aacgctgcgt ctcaaaaatc accttttccg gtcatacggt gaactcatcg gatatggcca
     7501 cgctgaaaat tatcggcgac gactaacgcc agaatataga agccacaaaa aatgaatgtt
     7561 aattacctga atgattcaga tctggatttt ctccagcatt gtagtgagga acagttagca
     7621 aatttcgccc ggctgctcac ccataatgaa aaaggcaaaa ctcgcctctc cagcgtactg
     7681 atgcgcaacg aactgtttaa atcgatggaa ggacatcccg agcaacatcg ccgcaactgg
     7741 cagctgattg ctggagaatt acagcatttt ggtggcgata gtatcgccaa caaactgcgc
     7801 ggacacggta aattgtatcg ggccattttg ctcgatgttt caaaacgatt gaagctgaaa
     7861 gccgacaaag agatgtctac gtttgaaatt gagcaacagt tactggaaca atttctgcgt
     7921 aatacctgga agaaaatgga cgaggaacat aagcaggagt ttctgcacgc ggtcgatgcc
     7981 agggtgaatg agctggaaga gctgctgccg ctgctgatga aagacaaatt attggcaaaa
     8041 ggcgtgtcgc atctgctttc cagccaactg acccgcattt tacgcaccca cgcagcaatg
     8101 agcgtacttg ggcatggttt gctgcgcggc gcggggctgg gaggccctgt aggcgcggca
     8161 ctaaatgggg ttaaagcggt cagcggcagc tcctatcgcg tgacgattcc agccgtactg
     8221 caaatcgcct gcctgcgccg gatggttagc gccactcagg tctgataccg gaaaccgggg
     8281 aaaattttca ttttcccctc aaaagatcgt agacactgcc ccactggctg attattatgc
     8341 cgcgccctga aaacactaca gttattcagg gaaattattt caccattcat tcgatgatga
     8401 tttttgagga attatgggca acactaagtt ggctaatccg gcaccgctgg gcctgatggg
     8461 cttcggcatg accaccattc tgcttaacct gcacaacgta ggttatttcg ctctggacgg
     8521 tattattctt gccatgggca ttttctacgg cggcatcgcg caaatttttg ctggtctgct
     8581 ggagtacaaa aaaggcaaca ctttcggttt aaccgcattc acctcttacg gttctttctg
     8641 gctgacgctg gttgcgattc tgctgatgcc gaaactgggt ctgaccgatg cgccaaatgc
     8701 acagttcctt ggtgtctacc tgggtctgtg gggcgtattt acgctgttta tgttcttcgg
     8761 cacgctgaaa ggcgcacgcg ttctgcaatt cgttttcttt agcctgaccg tgctgtttgc
     8821 cctgctggcg atcggtaaca ttgccggtaa cgccgcaatc atccactttg ccggctggat
     8881 tggtctgatc tgcggtgcca gcgcaatcta tctggcgatg ggtgaagtac tgaacgagca
     8941 gtttggtcgc accgttctgc cgattggtga atcccactaa ttgtcttgag atccccccgc
     9001 tcggggggat ttttttattc gctaacgtcg cgtcttgcac tcttcggtct gaatgcagca
     9061 accacttctg gtgccgtttc aacgtatggc ccttccagca actgaatgca gtacggtacg
     9121 ctggcaaaaa taccgtgtac cacaacgtta ccctcagcgt ccttcacacc ttccagcgtc
     9181 tctttaatag acttcggctg accgggtaag ttaaggatca gcgcctgttt gcgaatcaca
     9241 cccacctgac gcgaaaggat cgcagttggt acaaaatgca ggctgatctg gcgcatctgt
     9301 tcaccaaagc caggcatctc gcggtccgct actgccagcg tcgcatcggg cgttacgtca
     9361 cgacgcgccg ggccagttcc gcccgtggtg agcaccagat ggcaactcat ttcatccacc
     9421 agctcacaca acgtttgctc gatgatcgcc tgctcatcgg ggattaagcg ggtttccagt
     9481 tcaaacggcg tggttagcgc cgatgtcagc cattcttcca gcgcagggat acctttatcc
     9541 tgataaacgc cgctggatgc gcgatcagag atggaaacta agccaatacg taaagtattc
     9601 atcaagattt ttccgtttaa acagtctcgt taaacagaat gatacactgc gaagggagtg
     9661 acagacaggc ttcagaagag gtagcgtgac cgacttcccg gtcacgctaa gaatgattac
     9721 agcagatcgc cgatcatttt ttccagtttt tcctggtcaa tagcaaactt acggatacct
     9781 tccgccagtt tatctactgc cattggatcc tggttgtgct gccacaggaa ctcggactca
     9841 gtgatacgcg ccggacgcgc tttcacttcg ccggtgtaag acagtttacg ttcgatagcc
     9901 ccttcgctct cagccagctc tttcagcagt gccggtgcga tggtcagacg gtcgcagcct
     9961 gccagttcca gaatttcgcc gatgttacgg aagcttgcgc ccataaccac ggtttcataa
    10021 ccgtgctctt tgtagtactg gtagatttca gatacagaaa ccacgcccgg atcttctgcc
    10081 ggagcgtact ctttcttatc ggtattcgct ttgtaccagt caagaatacg gccaacaaac
    10141 ggcgagatca ggaacacgcc cgcttccgca caagcacgag cctgagcgaa ggagaacagc
    10201 agggtcaggt tacagttgat accttctttt tccagctgtt ccgcagcacg gataccctgc
    10261 caggtagaag ccagtttgat cagaatacga tcgttgctaa tacctgcatc gttgtagagt
    10321 ttgatcaggc gttttgcttt cgcaattgac gcttctgtgt cataggaaag acgcgcatca
    10381 acttcagttg agatacggcc cggaaccagt ttcaggattt ccagaccaat atttactgcc
    10441 agtttgtcag tcgcgtccac gatctgctgc gcgcgatcgt tgctctgctg tttcgcccag
    10501 gcgacagcat catcaatcaa cttacggtat tccggaatct gcgctgcgtt aagaatgaga
    10561 gaagggttgg ttgtggcatc ctgcggttga tacagcttca ttgccgcgat gtccccagtg
    10621 tcggccacta cggtggtgta ctgacgaagg gaggtcaatt tgtccgtcat gatagtattt
    10681 ctctttaaac agcttgttag ggggatgtaa ccggtctgcc ctgatgatat cacgacgcaa
    10741 cgtcagcgca accgccgaca agacggttat cgccaggcga gactgtttcg gatttctgac
    10801 actgcgttga tatcgcccgc catttttaaa caaaacctca tgtatgctac gcagaagtta
    10861 tcaagtacct cgtagcgtat atacttctta aacaattggt aacgtttaca caggaaagtc
    10921 atcgcgaccg gcaataagag ggatatgcat gccagatttt ttctccttta ttaacagcgt
    10981 cctttgggga tcggtaatga tttacctgct cttcggcgca ggttgttggt tcacttttcg
    11041 caccggattt gtgcagtttc gctacatccg ccagtttggc aaaagtctta aaaatagcat
    11101 tcatccacag ccaggcggtt taacctcatt tcagtcattg tgtaccagtc ttgcggcgcg
    11161 cgtgggtagc ggcaacctgg ccggcgttgc gctggctatt accgccggtg gacctggagc
    11221 cgtcttttgg atgtgggttg ccgcgtttat cggcatggcg acctcgtttg ccgaatgttc
    11281 ccttgcacaa ctttataaag aacgtgacgt caatgggcag tttcgtggcg gaccggcatg
    11341 gtatatggcg cgcgggctgg ggatgcgctg gatgggcgtt ctgttcgccg tctttttgct
    11401 catcgcctac ggcataattt tcagcggagt tcaggcaaac gctgttgccc gagctctgag
    11461 tttttctttt gattttcccc cgctggtgac aggcattatt cttgctgtct ttgctctgct
    11521 ggcaatcatt cgcggtcttc atggcgtcgc ccggctcatg caggggtttg tcccgttgat
    11581 ggcgataatc tgggtactga ccagcctggt gatttgcgta ataaatatcg ggcaacttcc
    11641 ccacgtcatt tggtctattt ttgagagtgc ttttggctgg caggaagcgg caggcggcgc
    11701 ggcgggatat accttaagcc aggcgattac taacggtttt cagcgcagta tgttttccaa
    11761 tgaggcggga atggggtcga cgccaaacgc ggcagcggca gcggcgtcct ggcctccgca
    11821 tccggcagcg caaggaattg tccagatgat tggcattttt atcgacaccc tggtcatctg
    11881 tacggcaagc gccatgctga tattactggc gggtaacggc acaacctaca tgccgctgga
    11941 aggtattcag cttatccaga aggcgatgcg ggtgctaatg ggttcctggg gtgctgaatt
    12001 tgttaccctc gtggttattc tgtttgcctt cagctccatc gttgccaact acatttatgc
    12061 cgaaaacaat ctcttctttt tacgcctgaa caaccctaaa gcgatctggt gtttgcggat
    12121 ctgcaccttc gcaacggtca tcggcggcac cttgctaagt cttccgctga tgtggcaact
    12181 ggcagatatc ataatggcct gcatggctat taccaatttg actgccatat tactgctctc
    12241 gcctgtggtt tataccattg ccagtgatta tctacgccag cgtaaactcg gcgtgcgccc
    12301 ggtgtttgat ccgttgcgtt atccggatat cggtcgccag ctttctcggg acgcgtggga
    12361 tgatgtttcg caggagtaat cacaactatc gatcaactca ttctcttttt ttgctaaagt
    12421 cggcataaat ttcctgcaag gactggatat gctgattctt atttcacctg cgaaaacgct
    12481 tgattaccaa agcccgttga ccaccacgcg atatacactg ccggagctgt tagacaattc
    12541 ccagcagttg atccatgaag cgcggaaact gacgcctccg cagattagca cgctgatgcg
    12601 tatcagcgac aaactggcag gtatcaacgc cgctcgcttt catgactggc agccagattt
    12661 cacgccggag aatgcccgcc aggcgattct ggcgtttaaa ggtgatgtct acaccggctt
    12721 gcaggccgaa accttcagcg aagacgattt cgattttgcc caacagcatt tgcgaatgct
    12781 ttccggcttg tatggcgtac tccgcccgct cgatttaatg cagccttatc gtctggaaat
    12841 ggggatccgt cttgagaata cccgagggaa agatctgtat caattctggg gagatatcat
    12901 caccaacaag ctgaacgagg cgctcgcagc acaaggcgat aatgtggtga ttaatctggc
    12961 gtcagatgag tattttaaat cggtgaagcc gaagaaattg aatgccgaga ttatcaagcc
    13021 ggtgttcctc gatgagaaga acggcaaatt taagataatc agcttctacg ctaagaaagc
    13081 acgcggtctg atgagtcgtt tcattattga aaatcggctg accaaaccag agcaactgac
    13141 tggttttaat agcgaaggtt acttctttga tgaagcttcc tccagcaatg gcgaactggt
    13201 gtttaaacgc tacgagcagc gttaagtttg tagtcaatgc cggatgcggc gtgaacgcct
    13261 tatccggcct acaaaagcat gcagattcaa tatattgcag aattcacgta ggcctgataa
    13321 gacgcgccag cgtcgcatca ggcgctgaat gccggattgt tacccggcat ttgtcattta
    13381 gcggtgatgt ttgcctggac catgaccgcc gtgatgatca tgatgagctt tcttatggtg
    13441 acgcggcggt ggcggcggtc cgtgtgggtg ccagcgattg cctcgccatt cataatgttg
    13501 tttccaccag ccgtggtcgc gccagtgacc gccatcccag taataaccgc gattatcacg
    13561 atcgcctatc tgtaatttta ctgacgggac taacgtaatt tccgcagcct gcgttgccat
    13621 gggagcgacc agaaccaggg aaagtgcgag tacgatagat tgcatctttt tcacaggtta
    13681 ctccttttat cattgcccgt tatgggtcga tatccggaga ttacgcgatg agagcggcgg
    13741 ttgttattct ccgcaatcct aatctctaag ttaccgcatt tattgagaat ttctcctttt
    13801 tccctgtcgt ttctctccat attttcttca caaaaaagcc gggctgcata aaagcaaacc
    13861 cggcctgatt gagataatga atagatgtta ctgatgattc atcatcaatt tacgcaacgc
    13921 agcaaaatcg gcgggcagat tatgcgaaag caagggtaaa tcggcacgtt ctgccagctc
    13981 ttttggcaga tccaacgttt caccgagaat cgcttccacg ctctctttaa atttcgccgg
    14041 atgcgcggtg ccgaggaaca agccatattc gcctggattc aactggtcac gcagcgcacg
    14101 atacgctacg gcagcgtgcg gctccgaagt gtagcccagt tcttttaact cacgcattgt
    14161 ctgttgcgtg gtttcatcat ccacggcggc ataacccagc tctttcagtt gccagatttt
    14221 gcggcggaac aactcttcca cacgcggcca gttgttcggc tgactcacgt ccatcgcgtt
    14281 ggataacgtc gcctgagtcg ctttgggtga ccactgaccg tcatgcagga aacgtggcac
    14341 ggtatcgttc acgttggtcg cagcaataaa acgtttcacc ggcagaccga gtgacttcgc
    14401 cagcagaccc gccgtcaaat cgccgaagtt tccgcttggc accgagacaa ccagctggtt
    14461 gcgcgcttcc tgcggcagct gcgcaactgc ttcaaagtag tagcaaatct gcgccagcaa
    14521 ccggctaatg ttaatcgagt tagctgagtt taaccccagc gccactttca gctcttcatc
    14581 atcaaacgcc tgcttcacca gcgcctgaca ggcatcgaaa tcgccgtcga tggcaacagt
    14641 ttcgatattg ccgcccaatg tacagaacag tttttcttgc agtggactga ttttgcctcg
    14701 tggataaagg ataaccactt tcacattcgg taaaccgtag aaagcatgag ccactgccgc
    14761 tccggtatca ccggaggtcg cggtcagaat ggtcactggc ttatcgcccg caatatgggt
    14821 cagcatttgt gccataaagc gaccgccgaa atctttaaat gccagcgttg gcccgtggaa
    14881 caattccaga caaccgacat cgctttcaac attggcgacc ggagccggga aggcaaacgc
    14941 cgcgcgcacg cgctcttcca ggatttcctg cgggatttca tcaccaataa acgccgagag
    15001 gatcttcgca ctgcgggtga caaaatccag cttcagcatc tcatcaattt cagtcaggct
    15061 gaattccggc aggtcgtgcg gaaaaaacag cccctgattt ttgcccaacc cctgggttac
    15121 ggcttgcgca aagctgacct gctcattgtg atctttaaga ttgtagagtt tcatttagtt
    15181 ttccagtact cgtgcgcccg ccgtatccag ccggcaaata tgaacaaaac cttcctgatt
    15241 ttgcaggtag ttcttaccca accagtcggc aacgcgctgg gcggtatccg gcttgtcaca
    15301 cagagcaaac aaggtcgggc cggagccgga gataccgctc gctaccgcgc cgatttccgc
    15361 gaccgcctgc cgcgcctgcc ggaagccagg cagtaaccgt tcacggtagg gttctgcgat
    15421 aacatctttc atcagcttcg cggcaagctc aggctgacgg gaatagcagg cgtgaatgaa
    15481 gcctgccaag tgtcgcccgt gcgcaatgca atcctggcgg cgatactgcg ccggtaaaat
    15541 agcccgggct tctgccgtcg agactttaat ccccggatac gccagcaccc acagccactc
    15601 atcaaaccct ggcacttgct ggctgatgat gtcgttttct tcgatcatca actgcatacc
    15661 accaagaaaa cacggtgcca cgttgtcgta atgaatgctg ccggagattc gtccttccag
    15721 ctcgcccatc aaagccagca aacgggtgtc attaagcggc ttgccgcagt gttcattcat
    15781 cgccatcaga gccgcgacca ccgaacaggc gctggagcct aagcccgaac cgatcggcat
    15841 attcttttcc agagtcatcg ccactggaat ttgcttgccc agctcctggc aaaaacgctc
    15901 ccagcactga taaacgatat tttcccgtgg ttctgacggc agcttatcgg caaagcgtcc
    15961 gaggttgttg agactgaatg tctctgccga ctcaaccgtg actacatctc cgagcaatgc
    16021 accatcaacg ggtgtcaccg ccgccccgag cacatcaaac ccgacgctca tattggcact
    16081 ggaagccggg gcataaactt taaccatgtc agactcctaa cttccatgag agggtacgta
    16141 gcagatcggc aaagacaccg gcagcggtaa cgtcattgcc cgcaccatat ccgcgcagca
    16201 ccaacggcag cggctgataa tagtggctat aaaaggccag ggcgttttcg ccatttttca
    16261 ctttgaacag cggatcatta ccatccactt cggcaatctt cacgcggcag acgccatctt
    16321 catcaatatt gccaacatag cgcaaaactt ttccttcatc acgggccttc gccacgcgcg
    16381 cggcaaagag atcgtcgagc tgtgacagat tcgccataaa agcggcaaca tcaccctcag
    16441 cgttaaactc tgcgggcagc acaggttcaa tttcaatatc cgccagctcc agttcacgtc
    16501 ccgtttcacg agcgagaatt aatagtttac gcgctacatc cataccagaa agatcatctc
    16561 gcggatccgg ttcggtataa cccatttccc gcgccagcgt agtcgcctcg gagaaactca
    16621 tgccttcgtc taacttgccg aagatataag aaagcgaacc tgaaagaatg ccggagaact
    16681 tcatcaattc atcaccagca ttgagcagat tttgcaggtt ctcaataacc ggtaatccag
    16741 ccccaacgtt ggtgtcatag aggaatttac gccgcgattt ttcagccgca tgacgcaaca
    16801 gatggtagta atccatcgac gaggtgttgg cctttttgtt cggcgtgaca acgtggaaac
    16861 cttcgcgcag gaagtcggca tattgatccg ccactgcctg gctggaggtg cagtcaacaa
    16921 tgaccgggtt cagcagatga tattctttca cgaggcgaat taagcgcccg agattaaacg
    16981 gctctttggc ttgcgccagt tcttcctgcc agttttccag atttaggcca tgcacattgg
    17041 tgagcagagc cttcgagtta gcaacaccgc agacacgtaa gtcgatatgt ttattcttca
    17101 accagctttg ctgacgcttc agttgctcca gcagcgcacc gccaacgcca ccgacgccaa
    17161 tcacaaacac ttcgataacc tgatcggtat tgaacagcat ctgatgagta acgcgcacgc
    17221 cagtggtcgc atcatcgtta tttaccacga cagagattga gcgttcagaa gatccctgag
    17281 caatagcgac aatgttgata ttggcgcggg ccagcgcggc aaagaatttc gccgagatcc
    17341 cacgcaaggt gcgcatacca tcacctacca ccgagataat ggccagccgt tccgtcaccg
    17401 ccagcggctc cagtaagcct tctttcagtt ccaggtagaa ctcttcctgc attgcccgtt
    17461 cagctcgcac acagtcgctt tgcggaacgc agaaactgat actgtattcg gaagatgatt
    17521 gcgtaatcag caccacggaa atacgggcgc gtgacattgc agcaaagacg cgcgccgcca
    17581 tgccgaccat tcctttcatc cccgggccgg aaacgctgaa cattgccata ttattcagat
    17641 tggaaatgcc cttgaccggt aattcgtctt catcacgact ggcaccaatg agcgtacctg
    17701 gagcttgagg atttccggta tttttaatca ggcaagggat ctggaactgg gcgatggggg
    17761 taatggtgcg ggggtgaaga actttagcgc cgaagtagga aagctccatc gcttcctggt
    17821 aggacatcga tttcaacaac ctcgcatcgg gcacctgacg cgggtcgcag gtatataccc
    17881 cgtcaacgtc cgtccaaatc tcgcaacaat cggcgcgtaa acaggcagcc agcaccgccg
    17941 cggagtagtc ggaaccgttg cgtccaagta ccaccagttc gcctttttca ttaccggcgg
    18001 tgaaacctgc catcagcacc atgtgatcag ccggaatacg acttgccgca atacggcggg
    18061 tggactctgc aatatcgaca gtagattcga ggtaatgccc cactgccagc agtttttcga
    18121 ccggatcgat aacggtaacg ttgtgaccgc gcgcttctaa tacgccggcc ataatggcga
    18181 tcgacatttt ctcgccacgg caaatcagcg cagcgttaat gctatccggg cactgcccta
    18241 acaaactaat gccatgcagg acatgtttta tttgggcaaa ttcctggtcg acgaaagttt
    18301 tcaattgcgc cagcgggaat cccggctggg cggcggcgag tcccgtcaga agttcggcaa
    18361 aaatacgttc ggcatcgctg atattgggta aagcatcctg gccgctaatg gttttttcaa
    18421 tcatcgccac caggtggttg gtgattttgg cgggggcaga gaggacggtg gccacctgcc
    18481 cctgcctggc attgctttcc agaatatcgg caacccgcag aaaacgttct gcatttgcca
    18541 ctgatgtacc gccgaacttc aacactcgca tggttgttac ctcgttacct ttggtcgaaa
    18601 aaaaagcccg cactgtcagg tgcgggcttt tttctgtgtt tcctgtacgc gtcagcccgc
    18661 accgttacct gtggtaatgg taatggtgat ggtggtggtg atggtggtgg taatggtggt
    18721 gctaatgcgt ttcatggatg ttgtgtactc tgtaattttt atctgtctgt gcgctatgcc
    18781 tatattggtt aaagtattta gtgacctaag tcaataaaat tttaatttac tcacggcagg
    18841 taaccagttc agaagctgct gtcagagact ctttttttaa tccacacaga gacatattgc
    18901 ccgttgcagt cagaatgaaa agctgaaaaa tacttactaa ggcgtttttt atttggtgat
    18961 atttttttca atatcatgca gcaaacggtg caacattgcc gtgtctcgtt gctctaaaag
    19021 ccccaggcgt tgttgtaacc agtcgaccag ttttatgtca tctgccactg ccagagtcgt
    19081 cagcaatgcc atggctcgtt cgcgtaaagc ttgcagttga tgttggtctg ccgttgtgtc
    19141 acttttcgtc ggttgttgta ttaatattgc taattgatag caatagacca tcaccgcctg
    19201 ccccagattg agcgaaggat aatccgccac catcggcaca ccagtaagaa cgtcagccaa
    19261 cgctaactct tcgttagtca gcccggaatc ttcgcgacca aacaccagcg cggcatggct
    19321 catccatgaa gatttttcct ctaacagcgg caccagttct actggcgtag cgtagtaatg
    19381 atatttcgcc cgactgcgcg cagtggtggc gacagtgaaa tcgacatcgt gtaacgattc
    19441 agccagtgtc gggaaaactt taatattatc aataatatca ccagatccat gtgcgaccca
    19501 gcgggtggct ggctccaggt gtgcctgact atcgacaatc cgcagttcgc taaaccccat
    19561 cgttttcatt gcccgcgccg ctgccccaat attttctgct ctggcgggtg cgaccagaat
    19621 aatcgttata cgcatattgc cactcttctt gaccaaatga ccgcgaacag ggtgatcact
    19681 gtcaacttat tacgcggtgc gaatttacaa attcttaacg taagtcgcag aaaaagccct
    19741 ttacttagct taaaaaaggc taaactattt cctgactgta ctaacggttt aattgttaaa
    19801 aaatgctaca tatccttctg tttacttagg ataattttat aaaaaataaa tctcgacaat
    19861 tggattcacc acgtttatta gttgtatgat gcaactagtt ggattattaa aataatgtga
    19921 cgaaagctag catttagata cgatgatttc atcaaactgt taacgtgcta caattgaact
    19981 tgatatatgt caacgaagcg tagttttatt gggtgtccgg cccctcttag cctgttatgt
    20041 tgctgttaaa atggttagga tgacagccgt ttttgacact gtcgggtcct gagggaaagt
    20101 acccacgacc aagctaatga tgttgttgac gttgatggaa agtgcatcaa gaacgcaatt
    20161 acgtacttta gtcatgttac gccgatcatg ttaatttgca gcatgcatca ggcaggtcag
    20221 ggacttttgt acttcctgtt tcgatttagt tggcaattta ggtagcaaac atgcagaccc
    20281 cgcacattct tatcgttgaa gacgagttgg taacacgcaa cacgttgaaa agtattttcg
    20341 aagcggaagg ctatgatgtt ttcgaagcga cagatggcgc ggaaatgcat cagatcctct
    20401 ctgaatatga catcaacctg gtgatcatgg atatcaatct gccgggtaag aacggtcttc
    20461 tgttagcgcg tgaactgcgc gagcaggcga atgttgcgtt gatgttcctg actggccgtg
    20521 acaacgaagt cgataaaatt ctcggcctcg aaatcggtgc agatgactac atcaccaaac
    20581 cgttcaaccc gcgtgaactg acgattcgtg cacgcaacct gctgtcccgt accatgaatc
    20641 tgggtactgt cagcgaagaa cgtcgtagcg ttgaaagcta caagttcaat ggttgggaac
    20701 tggacatcaa cagccgttct ttgatcggcc ctgatggcga gcagtacaag ctgccgcgca
    20761 gtgagttccg cgccatgctt cacttctgtg aaaacccagg caaaattcag tcccgtgctg
    20821 aactgctgaa gaaaatgacc ggccgtgagc tgaaaccgca cgaccgtact gtagacgtga
    20881 cgatccgccg tattcgtaaa catttcgaat ctacgccgga tacgccggaa atcatcgcca
    20941 ccattcacgg tgaaggttat cgcttctgcg gtgatctgga agattaatcg gctttaccac
    21001 cgtcaaaaaa aacggcgctt tttagcgccg tttttatttt tcaaccttat ttccagatac
    21061 gtaattcatc gtccgttgta acttctttac tggctttcat tttcggcagt gaaaacgcat
    21121 accagtcgat attacgagtc acaaacatca tgccggccag cgccaccacc agcacactgg
    21181 ttcccaacaa cagcgcgcta tcggcagagt tgaggagtcc ccacatcaca ccatccagca
    21241 acaacagcgc gagggtaaac aacatgctgt tgcgccaacc tttcaatacc gcctgcaaat
    21301 aaataccgtt cattaacgcc ccaatcagac tggcgattat ccatgccacg gtaaaaccgg
    21361 tatgttcaga aagcgccagc aagagcaaat aaaacatcac caatgaaagc cccaccagca
    21421 aatattgcat tgggtgtaaa cgttgcgcgg tgagcgtttc aaaaacaaag aacgccataa
    21481 aagtcagcgc aatcagcaga atggcatact tagtcgcccg gtcagttaat tggtattgat
    21541 cggcgggcgt cgtcactgcg acgctaaacg cggggaagtt ttcccagccg gtatcattgc
    21601 ctgaagcaaa acgctcaccg agattattag caaaccagct gctttgccat tgcgcctgaa
    21661 aacctgactc actaacttcc cgtttggctg gtagaaaatc acctaaaaaa ctgggatgcg
    21721 gccagttgct ggttaaggtc atttcgctat tacgccccgc aggcaccaca gaaagatcgc
    21781 cggtaccgct taaattcagt gccatattca gcttcaggtt ctgcttccgc cagtcccctt
    21841 cgggtaaagg gatatgcacg ccctgcccgc cttgctctaa cccgatgccg ggttcaatag
    21901 tcagcgccgt tccgttaact tcaggcgctt tcaccacacc aataccacgc gcatccccga
    21961 cgctaatcac aataaatggc ttgccaaagg tgatatttgg cgcgtcgagt tcgctaagac
    22021 gcgaaacatc gaaatcggct tttagcgtta aatcactgtg ccagacctga ccggtataaa
    22081 tccctatctt gcgttcttcc acgttctgat tgccatcaac catcaatgac tcaggtaacc
    22141 aaaaatggat aaaacttcgt ttccgctcca cggttttatc ctcttcctgc accgtataaa
    22201 gctcggtcac aggaatagcg ataagaggcc caacgagttt ttgcggcccg ctggtacttt
    22261 ggcgaatcgc atcttccaca tcgctacggt aatcggcacg ttcgacaatc acctgccgaa
    22321 tcagcattat cggaatcaac aacagcaata ctgcaccaaa cagggtggtc attttccaga
    22381 acagggggga tttcaacata gtcttctcct ttgcaatggc agcaggatac gaaggctatg
    22441 tgggaagaat ttgaagctat gtgaagtgac ggtgaagccg aagcgaggcc agcacgccac
    22501 cttcctgcac gttgcgcagc gtgacttcgc cgttaaacaa acgggcgacc tcactgacaa
    22561 acgccaaccc cagaccgctg cttttttgcc catttgcacg cggcaaagag taaaagcgtt
    22621 caaaaatacg tgacagcgcg tagtcaggaa taccactacc ggtatccagc accttaaggg
    22681 cgacgtgttc ctgatccact tcggcgctta gcgttatgcg accgctcttg ggggtaaaat
    22741 cgatggcgtt atccagtaaa ttccccagcg cctgctccag taacgccggt tcagcagcaa
    22801 cgttaacctc agtaggcata acatgcaaag tgatgttttt ttctgccaac tgcacggtgc
    22861 gcgcttcgct gacgcggcga aataatgccg ccacatcaac agcagtcaga acgacttcct
    22921 gacgattctc cagtcttgcc tggcgtagta acgtttctac caatgcctgc atacgcgcat
    22981 tttgcgtcag aatgttgtcg gtaaaacgag ccaccacttc cggtggcgga ccttcgcgta
    23041 aaatttccgc cgcgccacga atcgccgcca gtgggctttt tagctcatgg gttaacgcat
    23101 aaacatactg ctcaatatag tttttccctt ccagcttcac gcgcatactt tccagcgcct
    23161 gcgcaagctt acgcaactcg ctactgccga gttcaggaag aggaacgggc ttattgtcag
    23221 tgacggaatc ggcatagcga gtgagccggg caatggagcg gttgatccac caaaccatgc
    23281 ctgcgccaat caccagtgca atccccagca aaatggcgct ggcccataaa attcgccgct
    23341 cgctgcgctt aatgaccgga gccatcgccg cgttcggttt gcctacgctc aaaacgccaa
    23401 taagccgcga gccgtccata atcggcgcgg caacatacat caccgaactt tcgggatcgg
    23461 caggattttg caacgtgctg cgcgcaccat actgaccacg caacgttagc cagacgtcat
    23521 tccagcgcga ataatcctgc ccaacggctt tatttgccga atcgaacaat actttgccct
    23581 gcgcatcggt catatagaca tgatattcat tgcgcacttt gttaatgcca ccgatattag
    23641 cgcgaaacgg gcgatgttgt agctgattaa acgcctgcgc cagttgccca tgcgttgggt
    23701 ccccagagag caaatcggga cgcgccagct ccgccagcaa cgttgcggtg tcgatcagcg
    23761 tcccctccgt tgctcttcgc acgcccggtt taacttcttt gacaaaaata gccagtacga
    23821 accaggccgc caccgccacc agtaaaaaat agcccagcaa caaccgcatg ccgatacgca
    23881 ttacaggctc ctcaggctgt atcccatgcc gcgatgagta ttaatcggtg aaagatcggg
    23941 gttgatggcg cgcagcttgg cacgcagcgt tttaatgtgg gtatcgacgg tgcgatcgta
    24001 ggtgtcctgc gcatcttccc acacgatatc catcagttgc tggcgggacc agacgcggcc
    24061 tggcgacttg agtaacgtct tcagcaataa aaactcatac cgcgtcagcg ttaatggcgt
    24121 gtcaaaccag ctgatctgcg ccgcgggttc attcaattca aaatgaccaa tacggatgac
    24181 gggagacggc gtcgagaact tcttcacccg acgcagcaag gtgcgcaccc tagcgcacac
    24241 ttcgcggggt gaaaacggtt tagccacata gtcgtcagcg ccaatttcca gcccaagcag
    24301 gcgatcgacc tcttcacttc gggccgtcag gaacagtaca ggtaacgccg gatggagcgc
    24361 cagtaactgg cggcacaatt caaagccact gatatcaggc aaaccaacgt cgagaatcat
    24421 gacatcggga acctgctggc gagctttatc cagcaccggc aagcctcgct caaagacctc
    24481 gacggcaaaa ccttcctgct gcaacatgta gaccagcgtg tcggctatcc cttgctcatc
    24541 ttccactaac cagaccgttt cccgttgcat aaatcgcctc tgttattgtc gccacggcat
    24601 gacaggaacc gcgctaatcg cgtttttcgg cgaaccttct acaactttgt cggagtaagc
    24661 cagataagcg agcgcgttgc gtttggcgtc ataaaagcgc acgacctgta acgacttaaa
    24721 gaccagggac gtgcgttttt tgaatactac ctcgccctga gctttgccgt ttttaatacg
    24781 atccgacagt tcaatcggcc cgacttgctg acaagaaata gccgcatcgg aggtatcttc
    24841 cgccagaccc aatccccctt taataccacc tgttttcgcc cggctcacat aacaggtgac
    24901 atttttcaca tcgggatcgt caaaggcttc cacaacaatt ttgtgatccg ggccgatcat
    24961 tttaaatacg gtgtcgaccg aaccaatctc ttctgcatga gccaatggcc ccagcattat
    25021 caggcttaaa gacaggatca aatgcttgta tttcatattg ttaccatttt tatctattac
    25081 acgagcaatt agttcgtcac ggtaaataac atattgatta agaacaaaat ctcaatactt
    25141 ttatttccgt caagacctaa aacatactct actaagaaaa aaacactgaa tgctaaaaca
    25201 gcaaaaaatg ctattatcca attacctgat gtcaggtgct cgttgttgaa aggatgagga
    25261 tattttatgg atcaggccgg cattattcgc gaccttttaa tctggctgga aggtcatctg
    25321 gatcagcccc tgtcgctcga caatgtagcg gcgaaagcag gttattccaa gtggcactta
    25381 cagagaatgt ttaaagatgt cactggccat gctattggcg cgtatattcg tgctcgtcgt
    25441 ttgtcgaaat cggcggtcgc actacgcctg actgcgcgtc cgattctgga catcgcgctg
    25501 caataccgct tcgactctca acagacattt acccgcgcat tcaagaagca gtttgcccag
    25561 actcctgcac tttaccgccg ttctcctgaa tggagcgcct ttggtattcg cccgccgctg
    25621 cgtctgggtg aattcactat gccagagcat aaatttgtca ccctggaaga tacgccgctg
    25681 attggtgtta cccagagcta ctcctgttcg ctggagcaaa tctctgattt ccgccatgaa
    25741 atgcgttatc agttctggca cgattttctc ggcaacgcgc cgaccattcc gccagtgctc
    25801 tacggcctga atgaaacgcg tccgagtcag gataaagacg acgaacaaga ggtattctat
    25861 accaccgcgt tagcccagga tcaggcagat ggctatgtac tgacggggca tccggtgatg
    25921 ctgcagggcg gcgaatatgt gatgtttacc tatgaaggtc tgggaaccgg cgtgcaggag
    25981 tttatcctga cggtatacgg aacgtgcatg ccaatgctca acctgacgcg ccgtaaaggt
    26041 caggatattg agcgatacta cccggcagaa gacgccaaag cgggagatcg cccaattaat
    26101 ctgcgctgtg aactgctgat tcctatccgc cgttaacgct gcagctcatc taatgcaggg
    26161 gcgtccagat gcgagatgtc ccctgcggtt tcaacgaccc agccagacgc cagccacagg
    26221 ctttcctgat aatccacgcg cgaaatagaa cagttacgca gacgtaagcg gcgctctgcc
    26281 catgctggta atccgagaat cgtactcacc agacatccca gtgcaatacc atggctcacc
    26341 agcagcggtc ggcttccctg cggtaagtcc cggcaagatt ccagtgccgc attgacgcga
    26401 tcgctgagct cctgcattga ctcgccttca ggaatacgcc catcaacggt gccattgacc
    26461 agctgccgac gccagttctc ttcttcttcg gtcagagaat cgatatgtct tttttccagc
    26521 acacccatgt ttaattcacg caggcgagaa tcaaagatga tgtcacagcc gcaggcctgg
    26581 gcgatgattt ccgccgtacg ccgggtgcgt cctaaatcgc tactgatgat atgcgtaatg
    26641 ccaagctctt tggcacgcgt cgccacctgc atcgcctgtt gctcaccttt ggcggtcagc
    26701 gggctgtcag actggccctg aatacgtcgc tcggcgttcc actgcgtttc accgtggcgg
    26761 actaggtata cctgtaacat gctttttttc cgtatactgc tgtaacgtta attttctgag
    26821 taatgctgat tatgcaccaa gttgtctgtg cgaccaccaa tcccgctaaa attcaggcca
    26881 ttctgcaggc atttcacgag atcttcggcg aaggatcctg ccatattgca tccattgccg
    26941 tcgagagcgg tgtaccggaa cagccctttg gcagtgagga aacgcgcgct ggcgcacgaa
    27001 accgggtagc caatgcccgc cgtttacttc cagaggctga tttttgggtg gcgattgaag
    27061 ctggcattga tggcgacagc actttcagtt gggtggtgat tgaaaacacc acccagcgcg
    27121 gcgaggcgcg ttcggcaact ttgccgttgc ctgcggtgat attggaaaaa gtacgtgaag
    27181 gtgaggcgct tggtcccgtg atgtcgcgtt ataccggtat tgatgagatt ggtcgtaagg
    27241 aaggggcgat cggcgtgttt accgccggga aactcactcg cgccagcgtc tatcaccagg
    27301 cggtgatcct ggcgctgagt ccgtttcata atgcggtgta ttaagcgcct gatgcgacgc
    27361 tgccgcgtct tatcatgcct accaaacata ttgaaattac gggtatttgt aggacggata
    27421 aggcgttcac gccgcatccg gcattcggtg cacgatgcct gatgcgccac gtcttatcag
    27481 gcctacaaaa tcaatcgctt ttcagcaaca cctcttccag ccactggcgc agctccacgg
    27541 gcgcggcttt caggctgtta gatccacgcg taatcgtcgc gatgcctgcg ccgagttcat
    27601 tttttaactc acgctggctc atttcgccgc gcaacagctc ttcgacaata cgcacgcgag
    27661 tccccaacgc ttcgcgctca tctggcgtca gcatcaggtt taacaacggt aaatggagat
    27721 cgttttggta ggcattctta agcaggtcga caaaacgtaa ccactcctgg tgacgctgtt
    27781 ctgccatcgc tgctgaatag ggtgattgtt gggccataat atgtcgccat tgttagcggg
    27841 ggaagcaaaa tgcctccccc ggttgtactc gctaaagagt acgatagcat atcataaacg
    27901 tgcggatcag taacgacgtc cccattccgt ggcgctcatc aacgtcggtt tatcccccat
    27961 gaaatagcgg tagtaagcgt cataagccag cacgttcttc acataaccgc gcgtctcgga
    28021 gaacggaata ctctcgacaa atgccacagc gtcgatacgc ccggcgctgt tgccaagcca
    28081 ggttcgcacc cgtccaggtc cggcgttata agctgctgag gagaaaatac ggttattacc
    28141 aaattgctga taaacatatt gcaggtaact ggtgccaatg ttgatattcg tttccggatc
    28201 cagcaattgc ccagggctgc tataaccagg aatagagaac atcttcaccg tatgggtcgc
    28261 cgtgcccggc ataatctgca tcaggccgct ggcccctacc ggtgatttca ctttcggatt
    28321 ccaggcgctc tcctgacggg caatcgccat cgcatagctt tgcgggatct ccttcccact
    28381 ggtgtaacgt ttgaaaagat cgttgtaagc cagcgggaat cgctcttcca gatgatccca
    28441 aagcttcccg gcgatcgttg cctgaacgct aagatcccac cattggttgt tgaaagcata
    28501 ccgcgccagt tgagcctgct ctgtttttga cttgctcttc accagattgg cccactcgct
    28561 acgcgcggtg ttatcgagat tccagtacat caactcgcgc acgcgcgcca tctccggccc
    28621 ctgagtcagg gcgctgtcaa cattctgcgg cgctttatca atcttcagct catactcttc
    28681 gccgatgcgt tgtgcagcaa ccatcgggta gaaaccacgc tgttgcatga gttgatgcaa
    28741 aatctctttt gcttcagctt cacgtccgcg ttccagcaat aaatccgcct gccagtaacg
    28801 ccattcatct ttctctttcg cttccatagg cagacgcgcc agccaggtat tcaggccgcg
    28861 acgatcgcca gtgccaagcg ccattcgcac acggcgttca ataagcgaag tagattgcga
    28921 gcgcataatg gcgtcatcgc gccatttcgc ctgctcgtcg gtgacatcgt tgcccatcaa
    28981 acgccaggcg acgatatcgc gcagctcctg aatctgatct tcattaagct gctgcgcctg
    29041 ggcaagcgat gggatcatca gccgtgcatt ttcagcatcc tgccgcgcca cactggcaaa
    29101 cgccaccgcc gccatttgac gggtaaaatc ggtcgctccg gttgtacgcg cgaaggtcag
    29161 taccgtattg gggttgttcg ccagtgaaat gattgccgag gcgatagtct ggtaatcggc
    29221 aggcatctgc cctgccagca ctgttaccag gccagtgtta cccgctttca tcgccagacg
    29281 gatacgctct aaatacgcca gcggatcttg tttacctgac gcacgccaga cgctaaataa
    29341 cttgtcacag gcgttaggct ggctcttgcc ggttagccac agctctttcg ccccttgcca
    29401 ggcttcttca ctctgcccgg tgttccattt cgcatagtag taattacatt gtgcttcggt
    29461 agttccgggc ttttccgggc taaaggctaa caggccacgc cagtcttcac gccgcgccag
    29521 ttcattgacg aaacgagatt gcagcgtgcg agcgggagga agcgtggggt tagcgcgaac
    29581 aaagttagtg accgtcaccg ccggttgatt catcaggtca tcggtgatct ggcggtattc
    29641 caggtaggga taaagcggat aatccttcag tccaggcatc atttgttcga ccacatccat
    29701 ttgtcgatta tcccaggcct gcttgatttg cgcgtaacga ctacgctgct catccagtga
    29761 gtcggctcgc gccacgctgc tgaccgtcag cagacagaca ccggcagcca acagccgcca
    29821 ggtaacttgt ttggcttttt ccacaagcgc atcctctaag tgtaaataca tcaatgcagc
    29881 gtcatgccgc gtaatagatt tatgctaacc agtcattgcc gtttacgcca cgttacggac
    29941 acttttttac ttttactgcg agggtgatcg accggctggg attaggcggg gaaaaaggct
    30001 acacttcgcc tttgaaaact catcatcacg ataaacatag aggcgaagtc caacgtggct
    30061 caattcgttt ataccatgca tcgtgtcggc aaagttgttc cgccgaaacg tcatattttg
    30121 aaaaacatct ctctgagttt cttccctggg gcaaaaattg gtgtcctggg tctgaacggc
    30181 gcgggtaagt ccaccctgct gcgcattatg gcgggcattg ataaagacat cgaaggtgaa
    30241 gcgcgtccgc agccagacat caagattggt tatctgccgc aggaaccgca gctgaacccg
    30301 gaacacaccg tacgtgagtc cattgaagaa gcggtttcag aagtggttaa cgccctgaaa
    30361 cgcctggatg aagtgtatgc gctgtacgcc gatccggatg ccgattttga caagctggcc
    30421 gctgaacaag gccgtctgga agagatcatt caggctcacg acggtcataa tctgaacgta
    30481 cagctggagc gtgcggcgga tgcgctacgt ctgccggact gggacgcgaa aatcgctaac
    30541 ctctccggtg gtgagcgtcg tcgcgtggcg ttgtgccgcc tgctgctgga aaaaccagac
    30601 atgctgctgc tcgacgaacc gaccaaccac ctggatgccg aatccgtggc ctggctggaa
    30661 cgcttcctgc acgacttcga aggcaccgtg gtggcgatta cccacgaccg ttacttcctc
    30721 gataacgttg cgggctggat cctcgaactt gaccgcggtg aaggtattcc gtgggaaggt
    30781 aactactcct cctggctgga gcagaaagat cagcgtctgg cgcaggaagc ttcacaagaa
    30841 gcggcgcgtc gtaagtcgat cgagaaagag ctggagtggg tacgtcaggg aactaaaggc
    30901 cgccagtcga aaggtaaagc acgtctggca cgctttgaag aactgaacag caccgaatat
    30961 cagaaacgta acgaaaccaa cgaactgttt attccacctg gaccgcgtct gggcgataaa
    31021 gtgctggaag tcagcaacct gcgtaaatcc tatggcgatc gtctgctgat tgatgacctg
    31081 agcttctcga tcccgaaagg agcgatcgtc gggatcatcg gtccgaacgg tgcgggtaaa
    31141 tcgaccctgt tccgtatgat ctctggtcag gaacagccgg acagcggcac catcactttg
    31201 ggtgaaacgg tgaaactggc gtcggttgat cagttccgtg actcaatgga taacagcaaa
    31261 accgtttggg aagaagtttc cggcgggctg gatatcatga agatcggcaa caccgagatg
    31321 ccaagccgcg cctacgttgg ccgctttaac tttaaagggg ttgatcaggg taaacgcgtt
    31381 ggtgaactct ccggtggtga gcgcggtcgt ctgcatctgg cgaagctgct gcaggttggc
    31441 ggcaacatgc tgctgctcga cgaaccaacc aacgacctgg atatcgaaac cctgcgcgcg
    31501 ctggaaaacg ccctgctgga gttcccgggc tgtgcgatgg ttatctcgca cgaccgttgg
    31561 ttcctcgacc gtatcgccac gcacattctg gattaccagg atgaaggtaa agttgagttc
    31621 ttcgaaggta actttaccga gtacgaagag tacaagaaac gcacgctggg cgcagacgcg
    31681 ctggagccga agcgtatcaa gtacaagcgt attgcgaagt aatgcgtaaa atgccggatg
    31741 cggcgcgaac gccttatccg gcctacaaaa ccagcaattt caatacgttg taaaactgta
    31801 gacctgataa gacgcggtaa gcgtcgcatc aggcattgag caccgaatgc cggatgcggc
    31861 gtgaacacct tttccggtct acaacagcct tagcatcctc aatttcgtgg cgggccaccc
    31921 gtgcaggttt tttcaaacct tgtttaatct cgacagcctc ttccagagag gttttcaact
    31981 catcaaaaaa actcatttta tacctcgcct ttgagcagtt tcatcacggt tatctctgct
    32041 cccccatcat ctcccgcacc agctccacgc agcgcaggaa acggctgtcg taatcttcct
    32101 cttcaacccg cacaaattcg atattgttct cttccagcat ctccaccagc agattctgga
    32161 actctttacg atccaccgaa ctgccgaggc tgcgtaaacc atccaccacc cacggcgtgt
    32221 tgttctccag caggatcacc agatcgaaac ggtattcatc aatcagcgcc tgcacgaacg
    32281 gatgttcccg cccttcgtac tttttgcaga acgcctgagt ggtgacaaaa tcggtatcga
    32341 taaatgccac tttattggca tatttcaccg caaaatcaat gtattgagcg tggcccagcg
    32401 cgattttatc gtagtcagaa tactgcaatg cgatctcatc accgccgagg tgtgaaaaga
    32461 cataatcgcg gccatattcc cacgcactgg tggtgttgaa gatattggca agtttgttta
    32521 ccagggtgga tttaccgctc gactcgccgc caaggatcgc cacggtacgc acaaaaaacg
    32581 gcttcacttc ggtaggaata tattcccagt agcggaacgg gttttcgcgg atctgcgcac
    32641 cgctgatact cataaaggta cgtttcggat cgaccagcac cgtctcgatc cccagatgtt
    32701 ccatatactg tggcgcatcg gcttcttccg aggtgtagat cagatccggc tggatcccct
    32761 tttcagccat aaactttttg ataccgttgc tccacacatc ccagccgtgc ggatacggct
    32821 ccatgccctc ttcgttgaaa gcatgaatgc gaatattttt ctgatattta aaagtttgca
    32881 ataaccagcg cagacgatcc ggcacggtcg gctgctgcga catggcactg tcttcgaaca
    32941 acgcgcggtc acgggtatcg tcaaaaccca taatgatatg cagctcgtca acctggctac
    33001 aggcgcgctg gataaggtag atatgtccgg tatgcagtgg gtagaactta ccgaatacga
    33061 caccgatcgt tttcttctgc cggggaaact caagccccaa aaaacggtgc aacgcctcca
    33121 gcttttgcgc gctggggctt ttgattttgg cattcagtaa ctggcttaaa taccctttgg
    33181 tcataccgct ggcatcagct acctgctgta gcgtgcagcc ctgttgcttg atggcagttt
    33241 tcaggtaatc aaatgacgac atatctccct ccgtatttct cattataagt cgtcgaacac
    33301 gctaagcgcg tcggagagtt ttttaacgcc aaaaatctgc atcccttccg gcgctttttt
    33361 cggtacgtta gccgccggaa caatcgcccg gcgaaaaccg tgtttcgccg cttcagagat
    33421 tcgttcctga ccgctgggca ccgggcggat ctcccctgcc agcccgactt caccaaacac
    33481 caccagatcc tgcggtagcg gtctgtcacg caggctggaa accatcgcca gcagtaacgc
    33541 taagtcggcg ctggtttcgg ttacctttac gccgccgacc acgttcacaa acacatcctg
    33601 atcggccatt tgcagaccac cgtgacggtg caagacagcc agcaggattg ccagacggtt
    33661 ttgttccagc cccactgcca cgcggcgcgg attcgccatc atcgagtgat cgaccagcgc
    33721 ctgaatctcc accagcaacg gacgcgttcc ttcccacacc accatcacgg agctgccgga
    33781 ggtcacttca tctccgcgac ttaagaaaat tgccgaaggg ttgctgactt cacgcagccc
    33841 ctgttcggtc atcgcgaaga cgcccagctc attcaccgcg ccgaagcggt ttttatggct
    33901 gcgcaaggtg cgaaaacggg agtcggcatc gccatctaaa agcaccgaac agtcgataca
    33961 gtgttccagc actttcgggc cagccagcga accatctttg gttacgtgcc ccaccatgac
    34021 aatcgccaca ccgcgcgttt tggcgaagcg tgtcaaataa gccgccgttt cacgcacctg
    34081 cgccacgcta ccaggcgatg actgtacatc cgccatatgc atcacctgga tcgagtcaat
    34141 taccatcagc ttcggttgct cttcttcggc aatcaggcag atctgttcga tgctggtttc
    34201 cgacaacata ttgagattgt cagtcggcag gccaaggcga tgagcgcgca ttgccacctg
    34261 ttgcagcgac tcttcgccgg tgacatacag cgttttcatc tgctgggcca gtttgcacag
    34321 cgtttgcagt agcagcgtgg atttccccgc accagggtta ccgccaatca gaatggcact
    34381 tcctggcacc acgccgccgc ctagtacgcg gtcgaactct ttaaatccgg tggaaaaacg
    34441 cggcagctct tcaaggctga tatcggagag tttctggact tttgccaccc cggcgctacc
    34501 ggcatagccg ctgagacgct cgttacgcgc caccgtaggc gacgcagcaa gacgcacctc
    34561 ggtgatggtg ttccaggcat gacaggcact gcactgcccc tgccagcgcg gataatcggc
    34621 cccgcattca ttacaaacaa aggcgcgttt tggagctttt gccacggtgt acctcgttaa
    34681 tgctgtgccg cccgcaggat ggcgggcgag caattacttc tgattcaggc tgcctgagag
    34741 gatgcagaat acccccatca ggtcagcgtg acggatggtg acttccgcct tttcattcac
    34801 ttttggcttg gcatggtagg caatccccag ccctgccgct ttgatcatcg gcaggtcatt
    34861 ggctccatcg ccaatcgcca cggtctgcgc cagcgggatt tcatactcct gcgcgaggcg
    34921 agtcagagtt ttcgctttgt actgcgcgtc tacgatgtcg ccgatcacat tgccggtaaa
    34981 tttaccgtcc atgatctcca gttcattggc taccacggct gtcaggcgca gcttgtcgcg
    35041 caggtattca gcaaagaaag taaagccgcc ggaggcaatc gccactttcc agcccagcgt
    35101 ttccagcttg agcaccagtt gcgttaagcc tggcatcagc ggcagatttt cacgcacctg
    35161 ttgcagaata ttggcgtcag cgcctttcag cgtcgccacg cggctgcgca ggctggcggt
    35221 aaaatcgagt tcgccgcgca tcgcccgttc ggttacttcc gccaccatct cgcccgttcc
    35281 ggccagtttg gcaatttcat caatacattc aatctggatg gcggtggaat ccatgtccat
    35341 caccagcaaa cccggcgtgc gcaggtgcgg gatttttccc agcggcgcga catccagctg
    35401 cgcttcgtgg gccaggcgtg tagcccgtgc ggtgagtgaa cctgccagac gaatcacctg
    35461 ataatcttcc acgcaccagg cggcaacaat caccatcgcc gcacccagtt tgctctggta
    35521 ttgggtcaga cgctgtttat ccagcccacg accatacagc agccagccgc tacgacctgc
    35581 gtggtaatcc agtggcatca cttcatcacc acttaatgaa agaggcagac ccggccataa
    35641 agagacatct tcaggcaggt cgcaccaggt aatgttaggc attaaggctc ctgtaaaatc
    35701 gttcgaagca gggaaaataa cgcatgaggc taccttgtat ccattgcttc tggcaacatt
    35761 aagtctcaaa ttttcaaagg gtggaagatg gctcgcacaa aactgaaatt ccggctgcat
    35821 cgggcagtga ttgtcctgtt ctgtcttgcc ttgttagtgg cactgatgca gggggcgtca
    35881 tggtttagtc aaaaccacca gcgacagcgt aatccacagc tggaagaact ggcccgcacc
    35941 ctggcgcgtc aggtgacgct gaacgttgca ccgctgatgc gtaccgactc accggatgaa
    36001 aaacgcattc aggcgatcct cgatcagtta acggatgaaa gccgtatcct cgacgcgggt
    36061 gtgtatgacg aacaaggcga tcttatcgca cgttctggcg aaagcgtcga agtgcgcgat
    36121 cggctggcgc tcgacggtaa aaaagcaggc ggctatttta accagcagat tgtcgagcca
    36181 attgccggga aaaacggacc gctcggctat ctgcgcctga cactcgacac ccatacgctc
    36241 gccaccgaag cccaacaggt ggataacacc actaacattt tacgcctgat gttgctgctc
    36301 tcactggcaa tcggcgtagt gctgacccgc acgctgctac agggtaaacg cacccgctgg
    36361 cagcaatcgc ccttcctgtt aaccgccagc aaaccggtgc cggaagagga agaaagcgag
    36421 aaaaaagagt gccccattac tacaagaaag gaaatcgtta tgtccacatt acgcctgctc
    36481 atctctgact cttacgaccc gtggtttaac ctggcggtgg aagagtgtat ttttcgccaa
    36541 atgcccgcca cgcagcgcgt tctgtttctc tggcgcaatg ccgacacggt agtaattggt
    36601 cgcgcgcaga acccgtggaa agagtgtaat acccggcgga tggaagaaga taacgtccgc
    36661 ctggcgcggc gcagtagcgg tggcggcgcg gtgttccacg atctcggcaa tacctgcttt
    36721 acctttatgg ctggcaagcc ggagtacgat aaaaccatct ccacgtcgat tgtgctcaat
    36781 gcactgaatg cgctcggcgt cagtgccgaa gcgtccggac gtaacgatct ggtggtgaaa
    36841 accgccgaag gcgaccgcaa agtctcagga tcggcctatc gcgaaaccaa agatcgtggc
    36901 ttccaccacg gcaccttgct gctcaatgcc gaccttagcc gcctggcaaa ctatctcaat
    36961 ccggataaaa agaaactggc ggcgaaaggc attacttcag tgcgttcccg cgtgaccaac
    37021 ctcaccgagc tgctgccggg gatcccccat gagcaggttt gcgaggccat aaccgaggcc
    37081 tttttcgccc attatggcga gcgcgtggaa gcggaaatca tctccccgga caaaacgcca
    37141 gacttgccaa acttcgccga aacctttgcc cgccagagta gctgggagtg gaacttcggt
    37201 caggctccgg cattctcgca tctgctggat gaacgcttta gctggggcgg cgtggaactg
    37261 catttcgacg ttgaaaaagg ccatatcacc cgcgcccagg tgtttaccga cagcctcaac
    37321 cccgcgccgc tggaagccct cgccgggcga ctgcaaggct gcctgtaccg cgcggatatg
    37381 ctgcaacaag agtgcgaagc gctgttggtt gacttcccgg accaggaaaa agagctacgg
    37441 gagttgtcga cgtggatagc gggggcggta aggtaactcg ctgattaaaa tatccaagga
    37501 aggaaatatg atcgatctgg aaaaccagga acgggagatt atcaatttaa tgttcagcca
    37561 gcgtatttcc tggctagccg ccgtgcgtat caggcataaa ttatcgcttg ctgaagtaag
    37621 taaaatgtta ggtatctcga ttaattctct aaaacagatc gaaaagacgg aacggcttag
    37681 ctcaaacatc aaaagcaaaa tggcagaaat ttatggttgt ccaccagagt tgctgatatg
    37741 cccttcctgg atgacggctg aacataaatg aatagattcg tgaatagata cttgaatgga
    37801 ttttattttc tatccatatg aaaaataata gtaaatctat aaatcagttg ctattgatga
    37861 atagatacta aaaagccgga gcagtccccc actccggctt aacacgacaa tcgcctttca
    37921 gcgaaacaca attactcttt atcgcccagc agaacggatt ccagtgcgat tttgatcatg
    37981 tcgttgaagg tggtctgacg ctcagcggca gtggtttgct cgtgagtgcg gatgtggtca
    38041 gacacggtgc agatggtcag ggctttcgcg ccaaactctg cagccacgcc gtagatacca
    38101 gccgcttcca tttccacgcc gagaatgccg tatttttcca tcacgtcgaa catttcgccg
    38161 tccggagagt agaacaggtc agcggagaac aggttaccaa cgcgagcatc aatgcccagt
    38221 gctttagctg catctactgc gttacgcacc atgtcgaagt cagcgatagc ggcaaagtca
    38281 tggtctttaa aacggatgcg gttaactttg gaatcggtgc aggcacccat accgataacg
    38341 acgtcgcgca gttttacgtg cggcagaact gcgccacagg aacccacgcg gataattttc
    38401 ttcacgccga aatcggtgat cagttctttg gtgtagatgg agcaggacgg gatacccata
    38461 ccgtgaccca ttacggaaat tttgcggcct ttgtaagtac cggtgaagcc cagcataccg
    38521 cgaacgttgt tcacttcacg ggcatcttca aggaaagttt cagcaatata cttcgcacgc
    38581 agcgggtcgc ctggcatcaa aactacgtca gcgaaatcgc ccatttctgc attaatgtgt
    38641 ggggtagcca ttgttttatc ctttttgtga cataacaaag gcggagtcaa cgctccgccc
    38701 aaatccatca gaacatggct ttgccatatt ccatatcaga agtaccaaaa tattttgcca
    38761 gagtctggcc gatatccgcg aaggtttcac ggtgacccag tgagcccggt tttactttcg
    38821 ggccgtaaac cagtaccgga atgtgttcac gcgtgtggtc agtaccggtc caggtcggat
    38881 cacagccgtg gtcagcggtg aggatcagga tgtcgtcatc gcgcagcaga gacatcagct
    38941 ccggcagacg gcggtcgaac agttccagac ccgcagcata accggcgacg tcgcgacggt
    39001 ggccccagga agagtcgaag tcaacgaagt tggtgaagac gatggtgtta tcacccgctt
    39061 ctttcatctc tttgatggtg gcgtcaaaca gcgcgtccag gccagtcgct ttcacttttt
    39121 tggtgatacc gcagttggcg tagatgtctg caattttacc gaccgaaacc acctggccgt
    39181 gtttttcatc aaccagtttc tgcagcacgg tcggtgctgg cggctcaaca gccaggtcgt
    39241 gacggttacc ggtacgctgg aagttaccgg ctttgtcgcc gataaacgga cgagcgataa
    39301 cacgaccgat attgtagccg ccgttggtca gctcttcacg ggcgatttcg cacagttcgt
    39361 agagtttatc cagaccgaaa gtttcttcat ggcaggcaat ctggaacacg gagtcagcgg
    39421 aggtatagaa aatcggcttg ccggttttca tgtgctcttc gcccagttga tccagaatga
    39481 ccgtaccgga agagtggcag ttaccgaggt aacccggcag attagcgcgt tcgaccagtt
    39541 tatccagcag ctcttgcggg aagctgtttt cgtgatcgga gaaatatccc cactcaaaca
    39601 gaaccgggac accggcaatt tcccagtgac cagacggggt atctttaccg gatgacattt
    39661 cgtgcgccca tgcgtacgcg ccgataactt cagcgttgcc gtccattccc gccggaatga
    39721 aaccggtaga accttcgtgt gctttcgcca gccccagacg ggtcagattt ggcagattga
    39781 gcgggccttt acgaccgtta tcagcttcgc ctttggcaca agcttctgcg atatgaccca
    39841 gggtgtcagc cccgacgtca ccaaagcgtt ctgcatcttc tgtagcgccg atgccgaatg
    39901 agtccagcac cataataaat gcacgtttca tatgttctcc gtactcagtg cttcaaattt
    39961 aaatgatcag atcagtatac cgttattcgc tgatacggcg atagacagtt ggtgtgcttt
    40021 ccggtgcttt atcggcaagt ttaattgccg ctttcaccgc tttcgccgct tcctgccagc
    40081 tgttttcgtc tttcgcgtgg ataaccgcca gcggacgctg gccgtctacc tggtcgccca
    40141 gacgtgccat atcagtaaag ccgacgctgt aatcgatggt gtcagatgcc tgacggcgtc
    40201 cgccgcccat tgcaaccact gccatcccca gcgcgcgggt atccatttca ctgacaaaac
    40261 cttcggtatc agcatagact gctttcgtca gcatcgctgt cggcagatac ttcgcgtagt
    40321 tctcaacgaa gtcggtcggg cctttttgtg ccgctaccat acgaccaaag acttctgccg
    40381 ctttaccgtt gtccagcacc gcctgcaatt tcgcgcgcgc ttcggcgtca tctttcgcca
    40441 gtttgccgga gataagcatc tccacacaca gcgccatcgt gacatcaaac agacgcgggt
    40501 tacgatattc acccgtcagg aactgcaccg cttcacgtac ttcaaccgcg ttaccggcac
    40561 tggaggccag cacctgattc atgtcggtga gcagtgcggt ggtgcgcacg cctgcgccgt
    40621 tagccacgcc aacaatcgct tcggcaaggg cttcagagag ttcgtaggtc ggcataaacg
    40681 cgccgctacc cactttcacg tccatcacca gcgcgtccag accttccgcc agtttcttcg
    40741 ccaggatcga ggcagtgatc agcgggatgg agtccacggt tgcggtaata tcacgggtcg
    40801 cgtagaaacg tttatccgcc ggagccagtg agctggtctg accgataatc gccacgccga
    40861 cgtctttaat aatttcgcgg aaacggttgt catccgggaa aatgtcgaag ccagggatgg
    40921 attccagttt gtcgagcgta ccgccagtat gaccgaggcc gcgaccagag atcatcggaa
    40981 tatagccgcc gcaggctgcg accatcggcc ccaacatcag cgaagtcaca tcgccgacgc
    41041 caccggtgga gtgtttatca acaatcgggc cattcagatg caggcttttc cagtcgagaa
    41101 cggttcctga atctcgcatc gccatggtca gcgagacacg ctcaggcatt gtcatatcgt
    41161 ggaagaaaat ggtcatcgcg agggcggcaa tctgcccttc ggagatagtg ttgtcgcgaa
    41221 taccgttgat aaagaaacga atttcttcat cgctcagcgc atgaccatca cgttttttac
    41281 gaataatttc ttgtgcgaga aacaaggtaa cctcccgagg aaaagagtat gaggtggctt
    41341 ttgccggatg cgtctacgcc ttatccggcc tacgggtaga tacgtctcgt aggcctgata
    41401 agcgcagcgc atcaggcgta aagcatctta cttagtagct gctggcgctc ttaccgtcgc
    41461 cgtgacccag cgctttcagc aggcttgcca gcaggctgga agcaccaaag cggtagtgac
    41521 gcgcatcagc ccagtcagca ccgaacagtt catctgcgat ggcgagatat ttctgcgcat
    41581 cttccgcagt acgcacgccg cccgccggtt tgaaaccaac ggttttttct acgcccatat
    41641 cacggatcac ttccatcatg atgcgcgcgc tttccggcgt cgcgttcaca gccactttac
    41701 cggtagaggt tttgatgaag tccgcacccg ctttgatgga gatttcagac gctttacgga
    41761 tcagcgcttc gtctttcagt tcgccggttt cgatgatcac tttcagcagt acattcgctg
    41821 ccgcgcaagc ctctttacag gctttcacca ggtcaaaacc aacctgctcg ttacccgcca
    41881 tcagcgcgcg gtacgggaac acaacgtcaa cttcatcagc accgtaggcg attgccgcac
    41941 gggtttctgc cagcgcgatt tcgatgtcgt cgttaccgtg tgggaagttg gttaccgtag
    42001 caatacggat ttccggggtg ccctgctctt tcagtgtttt gcgagcaatc gggataaagc
    42061 gaggatagat acagatagcg gcggtattgc cgaccggagt tttggcctga tgacacagag
    42121 caattacttt ctcgtcggtg tcgtcgtcat tcagggtggt caggtccatc aatttcagtg
    42181 cacgcaggct gcttgctttc agatcagtca tttcattctc ctggcttgtc gccaataaaa
    42241 ttcaccttgc gagtttgtta gtattctaac atctactccg caacacactt cgatatacat
    42301 cacaattaag gaaatctact tacaagtttg catcactata atgcgatctg gttcaaataa
    42361 ttcactttca aatgaatgcg tcagtggtgg caaacgcatc aggatcaaag tgaacatcac
    42421 gaaacttctt acaatggcgc atcaccttcg gcataaaagg aaaagaagat gcccacttct
    42481 catgaaaatg cactgcaaca acgttgccag caaattgtca ccagcccagt gcttagcccg
    42541 gagcagaagc gccattttct ggcactggaa gcagaaaaca atctgcctta cccacagctt
    42601 cctgccgaag cccgccgcgc gctggatgaa ggtgtaatct gcgatatgtt tgaaggtcat
    42661 gcgccgtaca aaccgcgcta tgtcttaccc gattacgccc gttttctggc gaacggttcc
    42721 gaatggctgg agctggaagg cgcgaaagat cttgatgacg cactctctct gctgaccatt
    42781 ctttaccacc acgtaccgtc ggtcacatcg atgccggtct acctggggca actggatgcg
    42841 ttgttgcaac cgtatgttat aattctaaca caagacgaga tcgatgttcg aataaaacgt
    42901 ttctggcgtt acctcgacag aaccctgcca gacgccttta tgcacgccaa tatcggcccg
    42961 tctgattcgc ccattacccg cgcgatctta cgtgcagacg cagatttgaa gcaggtttca
    43021 ccgaacctga cctttatcta cgatcctgaa atcacccctg atgacctgct gctggaagtg
    43081 gcgaagaaca tctgtgaatg tagcaaaccg cacatcgcca acggtccggt gcatgataaa
    43141 attttcacaa aagggggcta cgggattgtg agctgttaca actcactgcc gctggcgggt
    43201 ggtggcagca cgctggtacg ccttaacctg aaagccattg ccgagcgcag cgaatcgctg
    43261 gatgacttct ttacgcgcac tctaccgcac tactgccagc agcagatcgc catcatcgat
    43321 gcgcggtgtg aattcctcta tcaacaatca cacttctttg agaatagctt cctggtgaaa
    43381 gaagggctga ttaaccctga acgttttgtg ccaatgtttg gcatgtatgg gctggcggaa
    43441 gcggttaact tgctgtgtga aaaagaaggg attgccgcgc gctacggtaa agaagccgcc
    43501 gcaaatgaag taggttatcg catcagtgca cagctggcgg agtttgtcgc caataccccc
    43561 gtgaaatatg gctggcaaaa acgcgccatg ttacacgcac agtcggggat cagttccgat
    43621 atcggcacca cgccgggcgc gcgtttgccg tatggcgatg agccagatcc gatcacccat
    43681 ctgcaaactg tcgcccgcat catgcttatt attattctgg catcagcgac attctgacgc
    43741 tcgacgaaac catcaaacgt aacccgcagg cgttggtaca gctttgcctc ggtgccttta
    43801 aagccggaat gcgtgaattt accgccaatg tcagcggtaa cgatctggtt cgcgttaccg
    43861 gttatatggt gcgtttgtcg gatttagaaa aatatcgcgc cgaaggttca cgcaccaaca
    43921 ccacctggct gggcgaagaa gccgcacgca acactcgtat tctggaacgc cagccgcgcg
    43981 tgataagcca tgaacagcag atgcgcttta gtcagtaaga ttatcccctt ctcctgcgtt
    44041 gacgggccag gcagtcgtct ggctctgttt ttgcagggct gcaatctgcg ctgcaaaaac
    44101 tgtcacaatc cgtggacgat gggacgttgc aatgactgtg gggagtgcgt gccacagtat
    44161 ccgcatcagg cgttgcagat tgttgacggc aaagtggtgt ggaacgctgt ggtctgcgag
    44221 caatgtgata cctgcctgaa gaggtgtccg caacatgcca cgccaatggc gcaatccatg
    44281 agcgtggatg aggtgcttag ccatgtccgc aaagcagtgc tgtttatcga agggataacg
    44341 gtgagtggcg gcgaagccac gacccaactg ccgtttgtag tggcgctgtt tactgctatc
    44401 aaaaacgatc cgcaactgcg ccacctcacc tgcctggtgg acagtaacgg tatgttgagc
    44461 gaaaccggct gggaaaaatt gctcccggtg tgcgacggcg caatgctcga tctcaaagcg
    44521 tgggggagcg aatgtcatca gcaattaacc ggacgcgata atcagcagat taagcgcagc
    44581 atctgtttgc tggcagagcg cggtaagctg gcggaactgc gtttgctggt aatccctggc
    44641 caagtggatt atttgcaaca catcgaagaa ctggcggcgt ttatcaaggg agtcggcgat
    44701 gttccggtac gcctgaacgc gtttcatgcc cacggcgtgt atggcgaggc gcaaagctgg
    44761 gcgagcgcca cgccggaaga cgttgagccg ttggctgatg cgttacggga gcgcggggtg
    44821 agccggttaa tatttccggc gctgtatttg tgacatgccg gatacgacct acggttcggc
    44881 acagacttgt aggcctgata agacgcgtca agcgtcgcat caggcattgt gcgccaactg
    44941 ccggatgcgg cgtgaacgcc ttatccggcc tacggttcgg cacaaacctg taggcatgat
    45001 aagacgcgtt aagcgtcgca tcaggcatta tgcgccgact gccggatgct gcctacgaag
    45061 ggcaaactac ccacgaacgt taaacaccgc atacgtgtta ttaagcaaca cttcggcaat
    45121 ctcatcttct ggttccgggc gcaactcgca aagcacgtca aacacccgcg cagcctgttc
    45181 cgggcggtta ggctgcccct gaaaaccgtt gagcggcata tccggcgcgt cggtttccag
    45241 caacaacgat gccagcggta attttgcgat gacaacgcgg gttttactgg cgcgtggata
    45301 ggtgatagta ccgcctacgc cgattttgta gcccagctgt acaaaccgct cggcctgttg
    45361 caggctgccg gaaaaaccgt gaaccacgcc agtgcgcgat aaatcgtggc gtttaagatg
    45421 catcgccagt ttgtcgtgtg tgcgccgtga atgcaggatc accggcagat cgtagcgttt
    45481 cgccagtttc agttgttcgt cgagaaacca ctgctgcctc tcaaattgcg gatcgtcgcc
    45541 gaagagatcc agaccgctct cccccaccgc caccaccttc gccggacgcc tttccagcgc
    45601 ctgctgtagc tgatcaagag acacatcgct atgtttttcc aacataccag gatgcaagcc
    45661 caatgcggca tacagcggtt gataattttc cgctaacgcc tgcacgcggg caaaattttc
    45721 cgcctcggtt gccggaacaa tgatcttgcc tacgcccgct tgtgccgcgc gttgcaggct
    45781 ggcctcttca tcgccaataa aaggcgggaa atcaaaatgg cagtgggtgt cgataaaacg
    45841 gcaaatcaag ccagatcctc attgttaaat gtcgggtcgt tcgcctgcgg agcgtcgctc
    45901 acttcggcaa ccagcgtatc gttggcgact ggcgcaggcg gaatgacaat cgattccggc
    45961 gtcaccactg gcaccagatg gcgggtaagc ggcgcttttt cagttaatag cttgccaacc
    46021 gtggcgagga aataacgacc gcataaacgc ccaagtttat agtcttcacg cagcgccgga
    46081 atccgactgc caagcgcgat actatgtaat ggcttcggcg gataaatttc gaatatccgc
    46141 agcttgcccg gtggtttctc aataaattgc tgaatgtcac gatagctggt ttcatgatgc
    46201 tgcaccagat tgaccagcgg ctgcaggcta ctgtcaccca gccagcgttc catgcgtttg
    46261 aaccactgcg gcgtgtagta catttgtgac ggcacagtgc ggatgacgac caacgtttta
    46321 gcgccctgcc ttgccgcctc tttaaccgga atcgcatcac tgatcccgcc atccaggtag
    46381 ttaatgcctt ccagcgacac tccgctacga taaaagccag gtatcgcact ggaggcgcga
    46441 atcacatcca gccagttttg tttggttggt aaaaagtaat tcggcgcgta gtcatcctga
    46501 cgacaggcgc acatataaaa cgatttgccg ctgtcaaaca accgcgcggc ggtatccatt
    46561 tgcaacggca tctggcttgc ggtggcctcc accagccagt cgagatcgat aagatttcct
    46621 ccacggacaa agcgcaatgg atcgaaaaat tcgcgttttg tggtatagcg catgatgact
    46681 ttgcgcgcgt aaccgggctg attgcagata tacgccgaga ggttctgcgc cccggcagat
    46741 gtgccgagat aaagatcgaa aggattaaac tgcgcgcgca taaactcatc cagcacgcca
    46801 gccgtgaaaa ttccacgctg tccgccgcct tcgcacacca gagctattcg tccaggctgg
    46861 aacggcctta gcgacaacgg cgcaatatta ccaagcgtta caggtattcg ctgccccacc
    46921 tctgccttcc tgtttttgtt gtgaaagaat gacaaagtaa cgcaatttat tattcgctaa
    46981 aaccgcaaaa gccagtccgc tggactggct ttggataaaa ttttcttaat ggtctgaaat
    47041 ctagggtcgt tttcggccca tgaacaaact caccaggaac agaataatcc cgacgacaaa
    47101 gacaatttta gctgcgcctg cagcggtacc ggccagacca ccaaacccaa gtgcggcggc
    47161 gattaacgcg ataaccagaa atatgatgcc ccaacgaaac ataagcgtct cctttaccat
    47221 agttaatgtc accgcttgat atgagcgtga aaatcactca atgcgatatt tttagtgtgg
    47281 tgcacatcgc gcctcccgac aaagttcggg aggacgaatt acgacgaatt acttagtttt
    47341 cagatcattt ttaacgcttt tcacaccatc taccgctttg gcgatacttt cagcacggtc
    47401 actttgtgcc tgagaatcta cgataccaga gagctgaacc acgccatcgg tggtttcaac
    47461 tttcacatga cgggaaggga cgatatcgtc cgccagcaat ttggctttga tttcactggt
    47521 ggtggcggtg tcacccgcgt agcccttcac agagccttct ttagcgtcgc gaacgtgcag
    47581 tttgtcgctg acagaggtca ccccttcaac acctttcgcc actttcactg cctcttcggc
    47641 ctgggcctgg ctttcaacga aaccgctcag ggtcacgact ttttgatcgg ttttgacaga
    47701 gatatcggtg ctcttgatgt tgtcatgatc caccagcgcc gccttcactt tcgcggtgat
    47761 ggcgctgtca tccatgaaat taccgacttt attcatagag ctatcgactt tttgccctgc
    47821 gctttcattg gtagtctgcg cgttgttttc cgcgtaggca gagccggtcg cgacggcaga
    47881 ggtcaacatt acagccagca gagttttcga aatcttcagt cttgtcatag tcatcgattt
    47941 attcctgtat gtttgctcgt aatttgagcc tggcaacacg aggttgcatt gctgaatagg
    48001 gagagacttc accctctaca gaagtcaatg gtcgccatca caaaagcgat gagtgatgaa
    48061 taacgaccat tacagcctct gaatcagtta ttaatatcgg tagaatgaca atcgacggct
    48121 ttagatactg atatctacgc attgaacggt atttaacgcc gtcagaaatg tcatcacttt
    48181 gttaaatata gatcacaatt ttgaaaccgc tcgggatatc agcgaaaaca taagcaaaag
    48241 tgaatgtttt aagaacattc cgtaagcggc taataaggaa gggaaattga caggacgcag
    48301 cggctaccgc gccctggagg caagaattaa tgctcgcggg tctggtggaa ctgaacgtcc
    48361 ggataacgtt cctgtgccag gcgcaggttg accatgctgg tagcgatgta agcgaggtta
    48421 tcgccgccat caagcgccag ttggctttcg ttcttacgct tgaactcttc gaatttcttc
    48481 gcgtctgcac attctaccca gcgggcagtg gcaacgttga ctgactcata cactgcttca
    48541 acgttgtatt cgctcttcag gcgcgctacc accacatcaa actgcagcac accaaccgca
    48601 ccaacgatca gatcgttgtt ggagattgga cggaacacct gcaccgcgcc ctcttcggaa
    48661 agctgtacca gccctttgag cagctgtttt tgcttcagcg gatctttcag gcggatacga
    48721 cggaacagtt ctggtgcgaa gttcggaata ccggtgaact tcatcatctc accctgggta
    48781 aaggtgtcgc cgatctgaat ggtgccgtgg ttgtgcaggc cgaggatatc gcccggatac
    48841 gcttcttcaa cgtgcgaacg gtcacccgcc ataaaggtca gcgcgtcgga gatcaccaca
    48901 tctttcgcag tgcgcacctg gcgcagcttc atgccttttt catatttacc ggacaccaca
    48961 cgcataaacg ccacgcggtc gcggtgtttc gggtccatgt tggcctgaat tttaaatacg
    49021 aagccggtaa atttatcttc gctcgcttct acggtacggg tatcggtctg acgcggcatc
    49081 ggcgcaggcg cccactccac caggccatcc agcatatgat caacgccgaa gttacccagc
    49141 gcagtaccga agaataccgg agtgatttcg cccgcaagga acagctcttt gtcgaactcg
    49201 ttagacgcgc ctttcaccag ttccagttcg tcacgcagct gctgtgccag atcttcacca
    49261 accgcagcat cgagatccgg gttattcagc cctttaacaa tgcggacttc ctgaatggtg
    49321 tgccctttac cgctctgata gagataggtt tcatctttat aaaggtggta aacgccttta
    49381 aacagcttgc cgcagccaat tggccaggtg atcggtgcgc agccaatttt cagctcgttc
    49441 tcaacttcat cgagcaattc catcgggtcg cggatatcac ggtcaagttt gttcataaag
    49501 gtgaggatcg gcgtgtcgcg cagacgggta acttccatca gcttacgggt acgatcttca
    49561 acaccttttg cggcgtcgat aaccatcagg cagcagtcca ccgccgtcag ggtacgatag
    49621 gtatcttccg agaagtcttc gtgccccggg gtgtcgagca ggttaaccag gcaatcgtga
    49681 tacggaaact gcatcacaga cgtagtaatg gagatcccac gctgcttttc catctccatc
    49741 cagtccgact tagcgtgctg gttggaacca cggcctttta ctgtaccggc ggtctgaatg
    49801 gcctgtccga acagcagcac cttctcggtg atggtagtct taccggcgtc cgggtgagaa
    49861 ataatggcaa aagtgcggcg cttcgccacc tcttgcaaat aaggagacaa cgtcataatt
    49921 ctttcttctt gagtaagcgg catcgcgccg cgcttgttga aaacgaaaaa ttgcggctat
    49981 tttacccatc aacggggggg aggcaatcag tgtttacaca ggagctgctc cagttcgtgc
    50041 aacgaagaaa cggtccaggt gggcgcgatg ccttctggtt gctcgcgatg gtgtgcattc
    50101 agccagcagg tcgcaagccc ggcgttgatg ccaccgagaa tatcggactc ggcagtgtcg
    50161 ccaaccatca gcacgcgtga acggtcagga ttgcccgcct gttccagcgc ataatcgaaa
    50221 attttcttat tcggtttggc aacgccaact tcttcggaaa tcaccagcaa atcgaagtaa
    50281 tcacgcaggc ccgtgcgttc cagacgcacc tgttgcaagg cactaaagcc gttggtgatg
    50341 atgccgattt tggcgttgcc acgaatggcg ttaagcagag aaaccgcgcc cggcagcggc
    50401 gtgcagattt ccgccatcgc attaataaag gcttcgttga gtttacctgg ctcgacgttc
    50461 agccgttcgg cccagctctc aaaccgcccg tgctgaagct gtaatgaagt gatcgcgccg
    50521 ttttgataat ccacccacag tggcttgtta acggcctgat agtcctgaaa atcttcagcg
    50581 gtaaaggtga cgctgtaatc aagaaacatc cgctgcaggc cggtgaatga gtcaaaggta
    50641 aacagcgttt catcggcatc aaagaaaatc cagtcccact tcattattcc accttgtctt
    50701 acatactgat tggcaacgcc atgatgatgg cgtcttcgcg accgtccgtg gtggggtagt
    50761 aattgcggcg aatcgtcgcc tcgttaaagc ctaaactttc gtacagggca atggcggcag
    50821 catttgaggc acggacttcc agccatagtg tcgccacgcc gcgtttttcc agttcgtcga
    50881 tcagatgttc cagcagcgcc cttcccaatc cctgacgctg atagtcagga tcgaccgcaa
    50941 tattgaacaa tgtagcttca tccagcacca cttgcgtaat cgcaaacgcc gccattttgc
    51001 cgttttgcgt taactgaaag ttgagataac gctcgccctg gttgctggca aaggtttttt
    51061 cactccacgg aaaggcgtgg gcgcgttgtt caatgtggta agccgctggt aaatcagtcg
    51121 tttcgaggga agaaatcgtg ttcatatgtg caaatttgtt gccataacgc ggcgcgtgcc
    51181 gttgggtttg cccgtaattc ggtgagcgcc ggtgatgcca cctgagcgcc ttccagtgat
    51241 agcggttcgt cagtacccaa ccgccagctg ttgcagcgac tgccttgcgg cagcatcgcg
    51301 attttttctg gcgtcagttg cagcacttga tcggggctga cggttaatgc gcgcagaaca
    51361 tcgctcacta aaggatcagt cagggcggga agatcgtttg ccaccatcac cagacggacg
    51421 tgtgccggga tcgcaatggc aatctccccc tgcaacgcgc cagggcgacg cagcgaccac
    51481 tgggtaatgc ccagttgctg taactgccag tctcgtcggg atgtcatagc gaatcgctcc
    51541 tgttgtcagg ggcgcaaata tagcaaattc gtcgataccg cgccaacata tggctataat
    51601 cgccgccagt atcaattgag gagcattcca tgtctgcatt taccccggca agtgaagtct
    51661 tgctgcgtca cagtgatgat ttcgaacaaa gccgtattct gtttgccgga gacttacagg
    51721 atgacctgcc cgcgcgttta gataccgcgg ccagccgtgc tcatacccag caattccagc
    51781 attggcaggt gttaagccgc cagatggggg ataacgcccg ttttagtctg gtcgccacgg
    51841 cggatgatgt cgcagattgc gatacgctga tttactactg gccgaagaac aaaccggaag
    51901 cccagttcca gttgatgaat ttactttctc tgcttccggt ggggacggat atttttgttg
    51961 ttggcgagaa ccgcagcggc gtgcgcagcg ccgagcagat gctggctgat tatgcgccgt
    52021 tgaataaagt cgacagcgcc cgtcgctgtg gcctctattt tggtcgtctg gaaaaacagc
    52081 cggtatttga tgccgataaa ttctggggcg aatacagcgt cgatggcctg acggtcaaaa
    52141 cgctgcctgg cgtgtttagc cgcgacggtc tggatgtcgg tagccagttg ctgctctcga
    52201 cgttaacccc gcacacgaaa ggtaaagtgc tggatgtcgg ctgtggcgcg ggcgtgcttt
    52261 cggttgcctt tgcgcgccat tcgccgaaaa ttcgtctcac cttgtgcgat gtctctgcgc
    52321 cagcggtaga agccagccgc gcaacacttg cggccaacgg tgttgaaggt gaagtctttg
    52381 ccagcaacgt cttttccgag gtgaaaggtc gttttgatat gatcatctcc aacccgccgt
    52441 tccacgatgg gatgcaaacc agcctggatg cggcgcaaac gctgattcgc ggtgcggtgc
    52501 gtcatcttaa tagcggcggc gagctgcgaa ttgtagcgaa cgccttcctg ccttatccgg
    52561 acgtgctgga tgagacattt ggcttccacg aagtgatcgc gcaaacaggg cgcttcaagg
    52621 tgtatcgcgc cattatgacc cgccaggcga agaaaggtta attatctcgc cgaataccgt
    52681 gtcggatgcg gcgtgaacgc cttatccgac ttacaaaacc tgcacgttag tctcctgatg
    52741 gacatttttc cagcaattac acatcaggcg ataattaact attgacgaaa agctgaaaac
    52801 cactagaatg cgcctccgtg gtagcaattc tttttaagaa ttgatggtat gcgaaggtgg
    52861 cggaattggt agacgcgcta gcttcaggtg ttagtgtcct tacggacgtg ggggttcaag
    52921 tcccccccct cgcaccaatt atctttactt cctttcttgt ttcttccctg ttttttcatc
    52981 cctgattcaa attcattatc atcagcatga tgccagcaat aagcgccagg cacgatggta
    53041 gcaaaatgta atgccgcaac tgtttgtgat acagcatcac cggcgtcaat actaatccca
    53101 tcagaatgac aatccgccac tgatccggcg ataaatccgc atacatcagc cctgcaatca
    53161 gaaatcccgg cagcaacact ccccagccac tgcccagcgt acgttgcaac atggttttat
    53221 ctctttcatt gataatgata accaatatca tatgataatt tttatcactt gcaagccaga
    53281 gaaatccctt gctatcgggt aaacctatcg ctaggattag caatcattat catttagatt
    53341 actatcccga ttatggccta tcgttccgca ccgctctatg aagatatcat ctggcgaacg
    53401 catctccagc cacaggatgc taggcttgca caagccgtac gtgcgacgat cgccgaacat
    53461 cgtgaacatt tgctggagtt tatccgcctg gatgaacccg ctccgcttaa cgccatgacg
    53521 ctggcgcaat ggtcatcacc gaatgcgcta agttctctac tggcggtcta ttccgatcat
    53581 atctatcgca accaaccaac gatgatccgc gagtacaaac cgctgatctc actatgggca
    53641 caatggtata tcggcctgat ggtgccacca ttaatgctgg cgctactgac gcaggaaaaa
    53701 gcattagatg tgtcgccgga acatttccat gctgagtttc acgaaaccgg acgcgtcgcc
    53761 tgtttctggg tcgatgtgtg tgaagataaa aacgcaacac cacattcgcc gcagcagcga
    53821 atggaaacgt taatcagcca ggcgctggtt ccggttgtgc aggcactaga agcgaccgga
    53881 gagatcaacg gcaaacttat ctggagtaat accggttatt tgatcaactg gtatcttact
    53941 gagatgaagc aactgctcgg cgaggcgacg gttgaatcgc tgcgccatgc cctctttttt
    54001 gagaaaacgc ttactaccgg tgaagataat ccactctggc gtaccgtggt gctacgcgac
    54061 ggcctgctgg tgcgccgcac ttgctgccag cgttatcgct taccggatgt acagcaatgt
    54121 ggcgattgta cgctgaaatg aaagcactgc cggggaagta aacccggcat catgcggatt
    54181 aatagggatg caacacatta cttgtttcgc aatgccgcat acataacaga atatctgcgg
    54241 cctccaacaa ccctgcgtca gaactgacgc ccagtttcga catcacatta aatttatgtg
    54301 cacggatcgt tttgatattg cgtttaagct gctcggcaat ttgtgtcatt gagtagccac
    54361 gcgacataaa gcgcaatatt tctctctccg tcgggcttaa cgtccggctt tggttaatgt
    54421 accactgatt gttcagtcgg tcggtcgcct gacgtacacc atttaatgac aaaaagagtt
    54481 cctgatgaaa aatctccagc gttgacgctt tacttaatac accgtccagc ggtgatggcg
    54541 acaatgaacc aatcagccga gcttcaatat catcatccgc aataactaaa cgccgggtac
    54601 gcggaaactt aatcgccagt tcagtcaggc aagataatcc ctctctgcgc tcacttctca
    54661 tggcagaaaa agaaaaaatg accgccgcaa acgagatccg cgacatggca tcctgaaatg
    54721 actcctgact ggtataggta tggagcttat aagggttgcc cgcaaacttt ctgaataaac
    54781 tctcaatacc gatactactc atgacgcatt tttctatgag tgcgacattt ctcttcttaa
    54841 ttcggctgtg ttccattctg cggcatctcc ataaacagaa ggcaagttaa gttctcttgc
    54901 accctgcgcg ctatttatcc atgcatacat ttcagcgttg ctgtgaattg ccagtcgacg
    54961 catcgcactg tttttttggg cgctgatagt tttattactt ttcttaagca atgaagctat
    55021 ctggttgatg ccccatcctt tacctaatag ccgcagtacc ttgcgctctg aaagagtgag
    55081 aatgacggag cgatagctaa aatcatgagt ttcagtcatt gccggagagg tcagcatggc
    55141 gctgatacgc tctgcgtgcg tattgccgga acgtacggtc ttgaccagat tctcaatggg
    55201 ttcaacatca gacaataacg tcgccgtagg gcacatgagc agttctacag cctgggaata
    55261 ccaagagcgt gacaccatga aaacccagtg aatttcccga tattgtgaga ttaaagaata
    55321 ataatgttca caaacagaac gtggatcttc actttcaccg gctaaatcag caatgactaa
    55381 tccggaacga cgtaattgta ataaggtaag gtcctccgca gaggcgctgg atattatttc
    55441 atattcaggg aagtgagtcc tcatgacctc ttttaaccct gcttgcataa caggtatttt
    55501 actg
//
LOCUS       DBFIFI010000012        48704 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.012, whole genome shotgun sequence.
ACCESSION   DBFIFI010000012 DBFIFI010000000
VERSION     DBFIFI010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48704
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.012"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            126..1343
                     /gene="alaA"
                     /locus_tag="N7B39_000727"
     CDS             126..1343
                     /gene="alaA"
                     /locus_tag="N7B39_000727"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HGU2420758.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPCGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1427..2026
                     /gene="yfbR"
                     /locus_tag="N7B39_000728"
     CDS             1427..2026
                     /gene="yfbR"
                     /locus_tag="N7B39_000728"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HGU2420759.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2085..3917)
                     /locus_tag="N7B39_000729"
     CDS             complement(2085..3917)
                     /locus_tag="N7B39_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HGU2420760.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4004..4654)
                     /gene="hxpA"
                     /locus_tag="N7B39_000730"
     CDS             complement(4004..4654)
                     /gene="hxpA"
                     /locus_tag="N7B39_000730"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HGU2420761.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4665..5159)
                     /locus_tag="N7B39_000731"
     CDS             complement(4665..5159)
                     /locus_tag="N7B39_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HGU2420762.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5242..5697)
                     /gene="yfbV"
                     /locus_tag="N7B39_000732"
     CDS             complement(5242..5697)
                     /gene="yfbV"
                     /locus_tag="N7B39_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HGU2420763.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6035..7237
                     /gene="ackA"
                     /locus_tag="N7B39_000733"
     CDS             6035..7237
                     /gene="ackA"
                     /locus_tag="N7B39_000733"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HGU2420764.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7312..9456
                     /gene="pta"
                     /locus_tag="N7B39_000734"
     CDS             7312..9456
                     /gene="pta"
                     /locus_tag="N7B39_000734"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HGU2420765.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIDSLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9646..11166
                     /gene="yfcC"
                     /locus_tag="N7B39_000735"
     CDS             9646..11166
                     /gene="yfcC"
                     /locus_tag="N7B39_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HGU2420766.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRLLTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11199..11741)
                     /gene="yfcD"
                     /locus_tag="N7B39_000736"
     CDS             complement(11199..11741)
                     /gene="yfcD"
                     /locus_tag="N7B39_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HGU2420767.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(11799..12350)
                     /gene="yfcE"
                     /locus_tag="N7B39_000737"
     CDS             complement(11799..12350)
                     /gene="yfcE"
                     /locus_tag="N7B39_000737"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HGU2420768.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12406..13050)
                     /gene="yfcF"
                     /locus_tag="N7B39_000738"
     CDS             complement(12406..13050)
                     /gene="yfcF"
                     /locus_tag="N7B39_000738"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HGU2420769.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLLFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLVLGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPECLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13186..13833
                     /gene="yfcG"
                     /locus_tag="N7B39_000739"
     CDS             13186..13833
                     /gene="yfcG"
                     /locus_tag="N7B39_000739"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HGU2420770.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAGLDYRLIKVDLGKGGQFRPEFL
                     LISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            13890..14252
                     /gene="folX"
                     /locus_tag="N7B39_000740"
     CDS             13890..14252
                     /gene="folX"
                     /locus_tag="N7B39_000740"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HGU2420771.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14273..15166
                     /locus_tag="N7B39_000741"
     CDS             14273..15166
                     /locus_tag="N7B39_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HGU2420772.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVMTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGIDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNKFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPIRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15214..16104)
                     /gene="rpnB"
                     /locus_tag="N7B39_000742"
     CDS             complement(15214..16104)
                     /gene="rpnB"
                     /locus_tag="N7B39_000742"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HGU2420773.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYLYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDVGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLEKGFD
                     HEVILTLTRLSPDDLIAQSH"
     gene            complement(16301..17074)
                     /gene="hisP"
                     /locus_tag="N7B39_000743"
     CDS             complement(16301..17074)
                     /gene="hisP"
                     /locus_tag="N7B39_000743"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HGU2420774.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17082..17798)
                     /locus_tag="N7B39_000744"
     CDS             complement(17082..17798)
                     /locus_tag="N7B39_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU2420775.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(17795..18481)
                     /gene="hisQ"
                     /locus_tag="N7B39_000745"
     CDS             complement(17795..18481)
                     /gene="hisQ"
                     /locus_tag="N7B39_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HGU2420776.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAARAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18571..19353)
                     /gene="hisJ"
                     /locus_tag="N7B39_000746"
     CDS             complement(18571..19353)
                     /gene="hisJ"
                     /locus_tag="N7B39_000746"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HGU2420777.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19574..20356)
                     /gene="argT"
                     /locus_tag="N7B39_000747"
     CDS             complement(19574..20356)
                     /gene="argT"
                     /locus_tag="N7B39_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HGU2420778.1"
                     /translation="MKKSILALSLLVGISAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20622..21191)
                     /gene="ubiX"
                     /locus_tag="N7B39_000748"
     CDS             complement(20622..21191)
                     /gene="ubiX"
                     /locus_tag="N7B39_000748"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HGU2420779.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21286..22803)
                     /gene="purF"
                     /locus_tag="N7B39_000749"
     CDS             complement(21286..22803)
                     /gene="purF"
                     /locus_tag="N7B39_000749"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HGU2420780.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIEAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(22840..23328)
                     /gene="cvpA"
                     /locus_tag="N7B39_000750"
     CDS             complement(22840..23328)
                     /gene="cvpA"
                     /locus_tag="N7B39_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HGU2420781.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGLLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(23860..24522)
                     /gene="dedD"
                     /locus_tag="N7B39_000751"
     CDS             complement(23860..24522)
                     /gene="dedD"
                     /locus_tag="N7B39_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HGU2420782.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVVPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(24512..25780)
                     /gene="folC"
                     /locus_tag="N7B39_000752"
     CDS             complement(24512..25780)
                     /gene="folC"
                     /locus_tag="N7B39_000752"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HGU2420783.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVCYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKTLPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(25850..26764)
                     /gene="accD"
                     /locus_tag="N7B39_000753"
     CDS             complement(25850..26764)
                     /gene="accD"
                     /locus_tag="N7B39_000753"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HGU2420784.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(26920..27579)
                     /locus_tag="N7B39_000754"
     CDS             complement(26920..27579)
                     /locus_tag="N7B39_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HGU2420785.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(27662..28474)
                     /gene="truA"
                     /locus_tag="N7B39_000755"
     CDS             complement(27662..28474)
                     /gene="truA"
                     /locus_tag="N7B39_000755"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HGU2420786.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28474..29487)
                     /locus_tag="N7B39_000756"
     CDS             complement(28474..29487)
                     /locus_tag="N7B39_000756"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HGU2420787.1"
                     /translation="MSEGWNIAVLGTTGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFVQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29553..30689)
                     /gene="pdxB"
                     /locus_tag="N7B39_000757"
     CDS             complement(29553..30689)
                     /gene="pdxB"
                     /locus_tag="N7B39_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HGU2420788.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHERTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADCGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            30788..31783
                     /gene="flk"
                     /locus_tag="N7B39_000758"
     CDS             30788..31783
                     /gene="flk"
                     /locus_tag="N7B39_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HGU2420789.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHCPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQSEAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMTLKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(31780..32958)
                     /locus_tag="N7B39_000759"
     CDS             complement(31780..32958)
                     /locus_tag="N7B39_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2420790.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALALTTMALPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(33233..34453)
                     /gene="fabB"
                     /locus_tag="N7B39_000760"
     CDS             complement(33233..34453)
                     /gene="fabB"
                     /locus_tag="N7B39_000760"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HGU2420791.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            34612..36618
                     /gene="mnmC"
                     /locus_tag="N7B39_000761"
     CDS             34612..36618
                     /gene="mnmC"
                     /locus_tag="N7B39_000761"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HGU2420792.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEARFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     ISQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNGFTFAR
                     RLYDSLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPEELAVAVEANAVEQITGVT
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQDLSRKDDCWLLTFAGDQ
                     QATHSVVVLANGHQISRFSQTSSLPVYSVAGQVSHIPTTPELAKLKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEETAYSEDNQQQNRQRLIDCFPHAQWAKTVDVSKKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDKAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(36739..37017)
                     /locus_tag="N7B39_000762"
     CDS             complement(36739..37017)
                     /locus_tag="N7B39_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HGU2420793.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(37051..37599)
                     /gene="epmC"
                     /locus_tag="N7B39_000763"
     CDS             complement(37051..37599)
                     /gene="epmC"
                     /locus_tag="N7B39_000763"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HGU2420794.1"
                     /translation="MNSTHHYEQLIEIFNSCFADDFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARREQVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(37599..38408)
                     /locus_tag="N7B39_000764"
     CDS             complement(37599..38408)
                     /locus_tag="N7B39_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HGU2420795.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(38408..39232)
                     /gene="mepA"
                     /locus_tag="N7B39_000765"
     CDS             complement(38408..39232)
                     /gene="mepA"
                     /locus_tag="N7B39_000765"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HGU2420796.1"
                     /translation="MNKTAIALLALLASSVSLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRRTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(39236..40321)
                     /gene="aroC"
                     /locus_tag="N7B39_000766"
     CDS             complement(39236..40321)
                     /gene="aroC"
                     /locus_tag="N7B39_000766"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HGU2420797.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSQVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(40356..41288)
                     /gene="prmB"
                     /locus_tag="N7B39_000767"
     CDS             complement(40356..41288)
                     /gene="prmB"
                     /locus_tag="N7B39_000767"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HGU2420798.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKAQLLAAREHFAIYKD"
     gene            41454..42005
                     /gene="smrB"
                     /locus_tag="N7B39_000768"
     CDS             41454..42005
                     /gene="smrB"
                     /locus_tag="N7B39_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311241.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HGU2420799.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKVSEVPV
                     KRLIQEQVDASHYFSDEFQPLLNADGPVKYVRPGVDHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(42176..43018)
                     /locus_tag="N7B39_000769"
     CDS             complement(42176..43018)
                     /locus_tag="N7B39_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HGU2420800.1"
                     /translation="MKKWTIFLTSLILLALSLETPKCYAGDKLMSASFSSTKIYYAMK
                     NVTASGSLYFYVTVVTPGEVSYGQYNSNARKGDTLKLISWSGSGPAPTLVLTDYRRTD
                     TSNCPGINTRVFVCAYMTFNVTVESDNYGCPWIASFYSVSEAFGFGTYTSPTVHDSIC
                     PTIPVASFDISWSDNYVSHNKALRLQSDGSTITTTLSTYLMESGKLCDGSIFDSRGAY
                     CRAVSDLLTFTSYGCDNAKVTVTPSRQPLTDRKLHDIVVQVNTSSREPIDSTCRFQYV
                     LNEL"
     gene            complement(43020..43541)
                     /locus_tag="N7B39_000770"
     CDS             complement(43020..43541)
                     /locus_tag="N7B39_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2420801.1"
                     /translation="MKNNRAWALISGLILFSGTAPAADNLHFTGNLLGKSCTPVINGN
                     LLAEIHFPTIAASDLMQRGQSDRVPLVFQLKDCKSTTAFNVKVTLMGTEDTDLPGFLS
                     IDSSSSATGVGIGIETAGGAAVPINSTTGASFPLNQGNNSVNFNAWLQTVNGRNVTSG
                     DFTATMTVTFEYF"
     gene            complement(43538..44008)
                     /locus_tag="N7B39_000771"
     CDS             complement(43538..44008)
                     /locus_tag="N7B39_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2420802.1"
                     /translation="MKRISLLMLWSFSFMALSNVSFHGYLVQPPNCSISNGQNIELTF
                     RDVNIDDINGSNYEQVVPYTITCDTAVRDPQMEMTLTWSGTQSDFDDSAVATDLNGLG
                     IHLKQAGSDFKLHTPLVVNETSLPVLTAVPVKKSGVDLPESDFEAWATLQVDYQ"
     gene            complement(44005..44505)
                     /locus_tag="N7B39_000772"
     CDS             complement(44005..44505)
                     /locus_tag="N7B39_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2420803.1"
                     /translation="MKGAYSAWAWLLLCSIVSTPVMAGSKSVAMVLRVLVDAPPPCTV
                     TGASVEFGNVFISKIDGVSYKRPIDYSLVCNNLAMDDLRLNMQATTTVINGETVIDTG
                     IAGFGIRIQKVSDHSILDLTPGAWLPFNFSSGALALEAVPVVQSGVSLTAAEFSASAT
                     IVVDYQ"
     gene            complement(44516..45274)
                     /locus_tag="N7B39_000773"
     CDS             complement(44516..45274)
                     /locus_tag="N7B39_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HGU2420804.1"
                     /translation="MTNLFCSARTAATTLALLITAASLSVQASVTPDRTRLIFNESDK
                     SISVTLRNNDPKMPYLAQSWMEDGQGNRISSPLTVLPPVQRIDSMMNGQVKVQGMPDI
                     NKLPADRESVFYFNVREIPPKSNKPNTLQIALQTRIKLFWRPKALENVSMKNPWQHKV
                     TLTLSGQEFTVNNPTPYFVVISNASAQKNGNPAAGFSPMVISPKTSVPLKVKMDSVPV
                     LTYVNDYGARLPLFFRCTGNTCQVDEEQNSKGQG"
     gene            complement(45297..47936)
                     /locus_tag="N7B39_000774"
     CDS             complement(45297..47936)
                     /locus_tag="N7B39_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HGU2420805.1"
                     /translation="MPDHSLFRLRVLPCCVALAMSGSYVNAWAENEIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLTEEYDIYWYASENDASKTYACLTPELV
                     AQFGLKEDVAKNLQWIHDGKCLKPGQLEGIDIKADLSQSALVISLPQAYLEYTDINWD
                     PPSRWDDGISGLIADYSITAQTRHEENGGDDSNEISGNGTVGVNLGAWRLRADWQTDY
                     LHSKSNDDDVINGDDTQKNWEWSRYYAWRALPSLKAKLGLGEDYLNSDIFDGFNYVGG
                     SISTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQLGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDINTASMPFLTRPGQVRYKLMMGRPQEWGHHVEGGFFSGG
                     EASWGIANGWSLYGGALADEHYQSAALGVGRDLSVFGAVAFDITHSHTRLDKETAYGK
                     GSLDGNSFRLSYSKDFDELNSRVTFAGYRFSEENFMTMSEYLDASDSEMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDRDEQTNYNVMLSHYFNLGSIRNMSISMTGYR
                     YEYDNQADKGVYISLSMPWGDSSTISYNGNYGSGSDSSQVGYFSRVDDATHYQLNVGT
                     SDNHSSVDGYYSHDGSLAQVDLSANYHEGQYTSAGISLQGGATLTAQGGALHRTQNMG
                     GTRLLIDADGVAGVPVEGNGAAVYTNMFGKAVVADVNNYYRNQAYIDLNNLPENAEAT
                     QSVVQATLTEGAIGYRKFSVISGQKAMAVLRLQDGSYPPFGAEVKNDSAQNVGLVDDD
                     GNVYLAGVKPGEHMIVSWGGVAHCDIHLPDPLPADLFNGLLLPCQQTGAISPSMPHEI
                     KPVIQEQTQQVMPTEAPVSVSAN"
     gene            complement(48018..48581)
                     /locus_tag="N7B39_000775"
     CDS             complement(48018..48581)
                     /locus_tag="N7B39_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2420806.1"
                     /translation="MSKFVKTAIAATMVMGAFASTSTIAAGNNGTARFYGTIEDSPCS
                     IVPDDHKLEVDMGDIGSGILKNNGTSTPKAFQIHLQDCVFDTQTTMTTTFTGNASSTN
                     SGNYYTIYNTDTGAAFNNVSLAIGDAQGTSYKSGAGIEQKIVNDTATNKGKAKQTLDF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        11957 a        13132 c        12164 g        11451 t
ORIGIN      
        1 cgagccttcc cctctggcaa aatcttattc tgcagacctt caaaacaccg tcctggggga
       61 gtacattgtt ctaagctgac ttccacggca gggagtggcg ataacagcaa aaaaggtcaa
      121 gattcatgtc ccccattgaa aaatccagca aattagagaa tgtctgttat gacatccgtg
      181 gtccggtgct gaaagaagca aaacgcctgg aagaagaagg taacaaggta ctgaaactga
      241 acatcggcaa cccagccccg ttcggttttg acgcgccaga tgaaatcctc gttgacgtga
      301 tacgcaacct gcctaccgct caagggtatt gcgattccaa aggtctttac tccgcgcgta
      361 aagccatcat gcagcactat caggctcgtg gcatgcgtga tgttaccgtg gaagatattt
      421 acatcggcaa tggtgtatcg gagcttatcg ttcaggcaat gcaggcattg ctgaacagcg
      481 gggacgaaat gttggttcct gcaccagatt acccactatg gaccgcggcg gtttcgcttt
      541 ccagcggtaa agcggtgcat tatctttgcg atgaatcctc tgactggttc ccggacctcg
      601 atgatattcg cgctaaaatt acgcctcgta cgcgtgggat cgttattatc aacccaaata
      661 acccaaccgg cgcggtatat tccaaagagc ttttaatgga gattgtggag attgcacgtc
      721 agcataatct cattatcttc gccgatgaaa tttatgacaa aattctctac gacgacgctg
      781 agcatcactc aattgcgccg ctggcacctg acctgctgac cattaccttt aatggactgt
      841 cgaaaacgta ccgcgttgca ggcttccgtc aggggtggat ggtgttgaac gggccgaaaa
      901 aacacgccaa aggctacatc gaaggtctgg aaatgctggc ttcaatgcgc ctgtgtgcta
      961 acgttcctgc gcaacacgcc attcagaccg cgctgggtgg ttatcagagc atcagtgaat
     1021 ttattacccc tggcggtcgt ctttatgagc agcgtaaccg cgcgtgggaa ttgatcaacg
     1081 atattccggg cgtttcctgc gtgaaacctt gtggtgcgct gtacatgttc ccgaaaatcg
     1141 acgccaaacg ctttaacatt cacgacgatc agaaaatggt gctggatttc ctgttgcagg
     1201 aaaaagttct gttggtgcaa gggacggcat tcaactggcc gtggccggat cacttccgca
     1261 ttgtcacgct accgcgtgtc gatgatatcg agctgtcgtt gagcaagttc gcgcgtttcc
     1321 tttctggtta tcatcagctg taatcttaat ttcactgccg gagattgcat ccggcagtgt
     1381 tatcccgcca caatgacctg atgatgtcat catacgtaag gtcactatga aacagagcca
     1441 tttttttgcc catctctccc gcctgaaact cattaaccgc tggccgctca tgcgcaacgt
     1501 gcggacggaa aatgtgtccg aacacagttt gcaggtagcg atggtcgccc atgcgctggc
     1561 agctatcaaa aatcggaaat ttggcggtaa tgtcaacgcc gaacgtatcg ctttactggc
     1621 gatgtaccac gatgcctcag aagtgctcac cggcgatctc cctactccgg tgaaatactt
     1681 caattcgcaa atcgctcagg agtacaaggc tattgaaaaa atcgctcagc aaaaactggt
     1741 cgatatggtt ccggaagagc tgcgggatat ctttgcgccg ttaattgacg agcatgcata
     1801 tagcgatgaa gaaaaatcgc tggtgaaaca ggcagatgca ctgtgtgcat atctgaaatg
     1861 tctggaagaa ctcgcggcgg gaaataatga attcttgctg gcaaaaacgc gactggaagc
     1921 gacgcttgaa gcgcgccgca gccaggagat ggactacttc atggaagtat ttgttcccag
     1981 cttccatctt tcgctcgatg agattagcca ggattcaccg ctgtaagcag ctggagtctg
     2041 cgtcgcatca ggcaataagc gccggatgcg acatcaggct cttgtcaaaa cggaaacagc
     2101 atcgggatca tcaccacaca aaccgccatc acgataatgg tgaacggtac ccccagcttc
     2161 acaaagtcac tgaagctgta atttcccgga cctaaaacca atgtgttaac aggtgaagaa
     2221 accggtgtca taaaggcggc ggatgctgcc atcgccacga ccatcgcgaa tggataaggc
     2281 gacaccccca tcgttttggc agcagccagc gcaatcggag ccatcaacac cgccgtcgcg
     2341 gtattagaga taaatagccc aataaccgcc gacaagacaa acaaacaacc cagcatcata
     2401 tgtggcccgt aaccaccgcc aatgtccatc agccctttca ccgccagcgc gacacctccc
     2461 gttttctgta atgccacagc aaacggcatc atcccaacga tcaaaataat gctcggccag
     2521 tgaatggatt tataggcgct ttcagcatct atacagcgga atttccccat cagcaggcag
     2581 gcgatgatag cggcaacagg attaggaatt tcatctgtca gcattaacgc caccattagc
     2641 accagacaga aaatggcatg gggtgcctgg ctgtgcgcgg gcgatgcttc actcacctct
     2701 tccggtaagt tcagcgctac gaagtcgcgg ccctgtttgg ccagcatacc gatcagtttc
     2761 cagttaccca caaccaggat gatatcgccc agcagcagag gctcatccgc cagcgaacct
     2821 tccagcgcca cgccattgcg ctttagcccc accacattca gtccgtagcg ggtacgaaaa
     2881 ccaatttcgc gcaccgattt accaatcagt tctgactcag gaattaatga aatctctgcc
     2941 atgcccacat caagggcctg gtcagaaaaa tactcgccgc gcagtaccat cggctccagc
     3001 aattgctcac tacaaaattg ccggagatcg acatcagccg cagacatatc aataagcaaa
     3061 acgtcacgcg cgcgaaattc agaaacccca ttaacgttca cgataacgcg acgaaaacgc
     3121 cgccagcgtt caacaccgat gacgttagcg ccataacgct cacgtaattt gagatcatcc
     3181 agccgttgac caatcattgg cgatccgggg cgaatagcca gacgtcgcgc acgcccggtc
     3241 agtcgatatt cacggataag atcgcgaaag gttcgacgcg tccagccttc gcgctgcggg
     3301 gtctgggtat cccctttcag catgaaacgc atcactaaca tatacaaaat acccagcacc
     3361 agcacgacca ggccaatagg tgttacgcta aagaaactga agccgtgata gccttcacgc
     3421 agcaattcac tgttgactac caggttcggc ggcgtcgcca ccagcgtcat catgccgcta
     3481 atcagcccgg caaaacttaa cggcatcatc agacgtgacg gcgaagtttg catacgcatg
     3541 gcaacgctta acaccacggg aataaagata gcgacaacgc cggttgaact cataaacgcg
     3601 ccaagccccg cgacggtcag catcaacaaa accaacattt tgatttcact gttgcccgca
     3661 actttgacca gccatgttcc cattacggtg gcaacaccgg tacggaccaa accatcgcca
     3721 ataataaaca aggcggcaat caggacaacg ttaggatcag aaaagccaga aaatacttct
     3781 gggactgtca gcgttccgct taatgcaaac gcgacaataa caaacaaagc gaccgcatcc
     3841 atacgcactc tgcccgtcgc aaacaagacg atggcaacgg ccagtaatga aagaacccaa
     3901 atcaattcac cgttcacaac ttatccttgt taattgaggg ggatgacttg attctgccat
     3961 aaaaaagccc cgacgagacg gggctaaatc atgatcaggt gtttcactga ataataacat
     4021 cgccatttgg ctgtttggtc acagtaattt gctccagact gtggaggacc aaatcgacct
     4081 cattcaggcg cggggtatct gccggagcgt taaccgcaat gacatggcaa cccgccgcca
     4141 ggccagaaag cacgccagcg ggagcatctt ccaccaccac acactcctgc ggcgcaagcc
     4201 ccagcagctg cgcgcctaac agatacgcat caggttctgg ttttccgcgc ttcactcgct
     4261 cagcggttac aaacacctct ggtgcgggaa gccccgctat tttatggcgc gctcgcgcta
     4321 ccggcatgga gccagaagtc acaatggccc acggaatacc tgctttattc aaatgattga
     4381 gtaaggcgat tgcccccgga agcgcggtaa taccttcggt ttccgtggcc tcgatttgct
     4441 ccaaacgcgt aaactcggcg gcaatatcag cctcggattt gcccgccata aaatggcgca
     4501 gagaggtgat cgcctgttta ccgtgaatga aagccagcac ctcttccggc gctaacccat
     4561 gacgtctggc ccagttgctc cacgcccgtt ctaccgcagg cagggaatcc accagcgttc
     4621 catcaagatc aaacagaaaa cctttgcacc gcacgcgggc ctcctcaggc gttgataatt
     4681 tgattaattt cgttggcgct caaatggtac tgacgcgggc aggcatgcca cacattaagc
     4741 atgcgctgat atttttccca cattggcgtc tgggcgttaa aaccgtgagt tccggcgtca
     4801 aaatgggtat agcgcccttc cacattaacc ataaagcgga cataaccgag gtaacgtgct
     4861 tcagtggcgg cgtcaaagcc gaggaaggtg acacgacgtt catcgatgga ttgctggtcc
     4921 tgcaaattag accaggaaac atgcaacgca tgatacatct ccataatgtc gatgatagtg
     4981 cggcaggttt cttctttcag ctcgccaaac tcgcgatcca gttcacgcat ctgtaatcca
     5041 taaccccgct caatgattgt ttgcaggcga cggtaacgtt cagcatttgc cggatcgagc
     5101 atagtcatca tcttgtactg gttagacaaa ataagacgtt gcgcgttggt catttccatt
     5161 gttgactcct gtatcactct actacggtga aaaaaaagaa ggctgagtat gccttctttt
     5221 atatgcgtaa tcaggggtca attacaaatc atcaaggaaa gttttatcca gttgtttgaa
     5281 ggcacgctta agcgtgtcag ctaatgcctg gtaatcaggc ttgccttcaa cgggtgccaa
     5341 cacctgtcca gactcctgca atttaccgcg aacttcataa aaccagttga ggattgcagg
     5401 gggtaatggc gtgacagaac gcttgcccag ccaccacaat ccctgcatgg gtaaacttaa
     5461 ggcgaacagg gcagtggcaa ctgccggccc aagctgaccg cccagggcaa tctgccagca
     5521 gagagtaaat acggcgatcg gcggcataaa acggatcgca taacgcgtca tcttgataac
     5581 gcgattttcg acaaagaccg gggcaaggcg tttttccagc ggccacgtct ttgagtaatg
     5641 ctgtccccgg cgaaacaagc taaaaaaatt aacagaacga ttatccggcg ttgacatgct
     5701 tcacctcaac ttcacatata aagattcaaa aatttgtgca aattcacaac tcagcgggac
     5761 aacgttcaaa acattttgtc ttccataccc actatcaggt atcctttagc agcctgaagg
     5821 cctaagtagt acatattcat tgagtcgtca aattcatata cattatgcca ttggctgaaa
     5881 attacgcaaa atggcataga ctcaagatat ttcttccatc atgcaaaaaa aatttgcagt
     5941 gcatgatgtt aatcataaat gtcggtgtca tcatgcgcta cgctctatgg ctccctgacg
     6001 tttttttagc cacgtatcaa ttataggtac ttccatgtcg agtaagttag tactggttct
     6061 gaactgcggt agttcttcac tgaaatttgc catcatcgat gcagtaaatg gtgaagagta
     6121 cctttctggt ttagccgaat gtttccacct gcccgaagca cgtatcaaat ggaaaatgga
     6181 cggcaataaa caggaagcgg ctttaggtgc aggcgccgct cacagcgaag cgctcaactt
     6241 tatcgttaat actattctgg cacaaaaacc agaactgtct gcgcagctga ctgctatcgg
     6301 tcaccgtatc gtacacggcg gcgaaaagta taccagctcc gtagtgatcg atgagtctgt
     6361 tattcagggt atcaaagatg cagcttcttt tgcaccgctg cacaacccgg ctcacctgat
     6421 cggtatcgaa gaagctctga aatctttccc acagctgaaa gacaaaaacg ttgctgtatt
     6481 tgacaccgcg ttccaccaga ctatgccgga agagtcttac ctctacgccc tgccgtacaa
     6541 cctgtacaaa gagcacggca tccgtcgtta cggcgcgcac ggcaccagcc acttctatgt
     6601 aacccaggaa gcggcaaaaa tgctgaacaa accggtagaa gaactgaaca tcatcacctg
     6661 ccacctgggc aacggtggtt ccgtttctgc tatccgcaac ggtaaatgcg ttgacacctc
     6721 tatgggcctg accccgctgg aaggtctggt catgggtacc cgttctggtg atatcgatcc
     6781 ggcgatcatc ttccacctgc acgacaccct gggcatgagc gttgacgcaa tcaacaaact
     6841 gctgaccaaa gagtctggcc tgctgggtct gaccgaagtg accagcgact gccgctatgt
     6901 tgaagacaac tacgcgacga aagaagacgc gaagcgcgca atggacgttt actgccaccg
     6961 cctggcgaaa tacatcggtg cctacactgc gctgatggat ggtcgtctgg acgctgttgt
     7021 attcaccggt ggtatcggtg aaaatgccgc gatggtccgt gaactgtctc tgggcaaact
     7081 gggcgtgctg ggctttgaag ttgatcatga acgcaatctg gctgcacgtt tcggcaaatc
     7141 tggtttcatc aacaaagaag gtacccgtcc tgcggtggtt atcccaacca acgaagaact
     7201 ggttatcgcg caagacgcga gccgcctgac tgcctgattt cacaccgcca gctcagctgg
     7261 cggtgctgtt ttgtaacccg ccaaatcggc ggtaacgaaa gaggataaac cgtgtcccgt
     7321 attattatgc tgatccctac cggaaccagc gtcggtctga ccagcgtcag ccttggcgtg
     7381 atccgtgcaa tggaacgcaa aggcgttcgt ctgagcgttt tcaaacctat cgctcagccg
     7441 cgtaccggtg gcgatgcgcc cgatcagact acgactatcg tgcgtgcgaa ctcttccacc
     7501 acgacggccg ctgaaccgct gaaaatgagc tacgttgaag gtctgctttc cagcaatcag
     7561 aaagatgtgc tgatggaaga gatcgtcgca aactaccacg ctaacaccaa agacgctgaa
     7621 gtcgttctgg ttgaaggtct ggtcccgaca cgtaagcacc aatttgccca gtctctgaac
     7681 tacgaaatcg ctaaaacgct gaatgcggaa atcgtcttcg ttatgtctca gggcactgac
     7741 accccggaac agctgaaaga gcgtatcgaa ctgacccgca acagcttcgg cggtgccaaa
     7801 aacaccaaca tcaccggcgt tatcgttaac aaactgaacg caccggttga tgaacagggt
     7861 cgtactcgcc cggatctgtc cgagattttc gacgactctt ccaaagctaa agtaaacaat
     7921 gttgatccgg cgaagctgca agaatccagc ccgctgccgg ttctcggcgc tgtgccgtgg
     7981 agctttgacc tgatcgcgac tcgtgcgatc gatatggctc gccacctgaa tgcgaccatc
     8041 atcaacgaag gcgacatcaa tactcgccgc gttaaatccg tcactttctg cgcacgcagc
     8101 attccgcaca tgctggagca cttccgtgcc ggttctctgc tggtgacttc cgcagaccgt
     8161 cctgacgtgc tggttgccgc ttgcctggct gccatgaacg gcgtagaaat cggtgccctg
     8221 ctgctgactg gcggctacga aatggacgcg cgcatttcta aactgtgcga acgtgctttc
     8281 gctaccggcc tgccggtatt tatggtgaac accaacacct ggcagacttc tctgagcctg
     8341 cagagcttca acctggaagt tccagttgac gatcacgagc gtatcgagaa agttcaggaa
     8401 tacgttgcta actacatcaa cgctgactgg atcgattctc tgactgccac ttctgagcgc
     8461 agccgtcgtc tgtctccgcc agcgttccgt tatcagctga ctgaacttgc gcgcaaagcg
     8521 ggcaaacgta tcgtactgcc ggaaggtgac gaaccgcgta ccgttaaagc agccgctatc
     8581 tgtgctgaac gtggtatcgc aacttgcgta ctgctgggta atccggcaga gatcaaccgt
     8641 gttgcagcgt ctcagggtgt agaactgggt gcaggcattg aaatcgttga tccagaagtg
     8701 gttcgcgaaa gctatgttgg tcgtctggtc gagctgcgta agaacaaagg catgaccgaa
     8761 accgttgccc gcgaacagct ggaagacaac gtggtgctcg gtacgctgat gctggaacag
     8821 gatgaagttg atggtctggt ttccggtgct gttcacacta ccgcaaacac catccgtccg
     8881 ccgctgcagc tgatcaaaac tgcaccgggc agctccctgg tatcttccgt gttcttcatg
     8941 ctgctgccgg aacaggttta cgtttacggt gactgtgcga tcaacccgga tccgaccgca
     9001 gaacagctgg cagaaatcgc gattcagtcc gctgattccg ctgcagcctt cggtatcgaa
     9061 ccgcgcgttg ctatgctctc ctactctacc ggtacttctg gtgcaggtag cgacgtagaa
     9121 aaagttcgcg aagcaactcg tctggcgcag gaaaaacgtc ctgacctgat gatcgacggt
     9181 ccgctgcagt acgacgctgc ggtaatggct gacgttgcga aatccaaagc gccgaactct
     9241 ccggttgcag gtcgcgctac cgtgttcatc ttcccggatc tgaacaccgg taacaccacc
     9301 tacaaagcgg tacagcgttc tgccgacctg atctccatcg ggccgatgct gcagggtatg
     9361 cgcaagccgg ttaacgacct gtcccgtggc gcactggttg acgatatcgt ctacaccatc
     9421 gcgctgactg cgattcagtc tgcacagcag cagtaacctc gtcatcatcc gcagctttgc
     9481 gctgcggata tctgaaccgg aaatagtcac tatttccggt tttttattct cttaatttgc
     9541 attaatcctt tctgattatc ttgcttaact gcgctgcatc aatgaattgc gccatctcac
     9601 tttgcatact taccactttg ttttgtgcaa gggaatattt gcgctatgtc cgcaatcact
     9661 gaatccaaac caacaagaag atgggcaatg cccgatacgt tggtgattat cttttttgtt
     9721 gctattttaa ccagccttgc cacctgggta gttccggtgg ggatgtttga cagtcaggaa
     9781 gtgcagtatc aggttgatgg tcaaacaaaa acacgcaaag tcgtagatcc acactcattt
     9841 cgccttctga ctaacgaagc aggcgaacct gagtatcacc gcgtacagct gttcacgacg
     9901 ggcgatgaac gcccgggcct gatgaacttc ccgtttgaag ggttaacctc aggatcgaaa
     9961 tacgggacag ccgttggcat catcatgttt atgctggtga ttggcggcgc gtttggcatt
    10021 gtgatgcgta caggaaccat tgataacggt atcctggcgc ttattcgcca tacccgtggg
    10081 aatgaaattc tctttattcc tgcgctgttt attctgtttt cacttggcgg cgcggtattt
    10141 ggtatggggg aagaggccgt cgcctttgcc attatcatcg caccgctaat ggtccggctg
    10201 ggctatgaca gtattaccac cgtcctggtg acctatattg ccacgcaaat cggttttgcc
    10261 agttcgtgga tgaacccgtt ttgtgtggtc gttgctcagg ggattgccgg cgttccggtg
    10321 ctttctggct ccgggttgcg catcgtggtg tgggttatcg ccactctgat tggcctgatc
    10381 tttaccatgg tgtacgcctc acgagtgaaa aagaatcctc ttctgtcacg cgtgcatgag
    10441 tccgaccgct tctttcgtga aaagcaggcg gatgttgaac aacgtccgtt tacctttggt
    10501 gactggctgg tattgattgt cctgaccgcc gtaatggtct gggtgatttg gggcgtgatc
    10561 gttaatgcct ggtttattcc agaaattgcc agccagttct tcaccatggg tctggtgatt
    10621 ggcatcatcg gcgttgtttt ccgccttaac ggtatgacgg ttaataccat ggcttcatcc
    10681 ttcaccgaag gggcgcgaat gatgatcgcc cctgccctgc tggtgggttt cgccaaaggg
    10741 attttgctgc tggtcggtaa tggtgaagcg ggtgatgcca gcgtgttaaa tactatcctc
    10801 aacagcattg ccaatgccat tagcggtctg gacaacgcgg tcgcggcctg gtttatgttg
    10861 ctcttccagg cggtatttaa tttcttcgtg acgtccggtt ctggtcaggc ggcgttaacc
    10921 atgccgttac tggcaccgct tggcgatctg gtcggtgtta accgtcaggt taccgtgctg
    10981 gctttccagt ttggtgatgg cttcagtcac atcatttacc cgacctcagc ttcgttaatg
    11041 gcgacgctcg gtgtttgccg ggtggacttc cgtaactggc tgaaggtggg cgcgaccctg
    11101 cttggactgc tgtttattat gtccagcgtc gtggtgatcg gcgctcagtt gatgggctac
    11161 cactaaaaat gttaagagcc gcaattgcgg ctcttttttc attctgccgt ttcagtctct
    11221 acggcttcat ttttggcatt gcgcttcatc cacaacgcta gcgctttcag cgagtctgga
    11281 gtgaactcat cgcagcgtgc ggtgatttct tccggcgtca gccagcaaac ttcactgact
    11341 tcatcttcct gtagggcgaa gggaccgtga gagacgcagc tgaacaatgc gccccagaca
    11401 cggcaatttt tatcttcgaa atagaactgc ccgtgctcgg caaaggggac accggcaatg
    11461 cccaactctt cttccgcttc gcgacgcgcg gattccagca gttgctcatc ggcctggact
    11521 acaccgccag cggtcgcatc taacatgccg ggtaaaaagt cttttgtctc ggtacgacgc
    11581 tggaccagaa ttttgcccat gccatcatgc acgacgatgt aagttgcacg atgacgcaga
    11641 cactgtgccc gcatttgctc ccggctggct tgtgcaatga cttcgttctc ttcattgaca
    11701 atatccaccc attcagtact tgccaaacga cgctgttcca ttatcaggaa accttctttt
    11761 tctggcgctc ttacggcgca ttttgagttg tgggtaaatt acggattaat cgcgacctgc
    11821 gcaatgatac tttgatcatt gagtgcgatt acactaagta cgtcattatc cagcatgcca
    11881 taactcgccg gattaccgcc tttcggaata ctcaccgagc cggggttgaa gtggaaaatc
    11941 tccccccgct gttctgctac tggcagatgg gtatgaccgt aaaccagcac atcgttctgg
    12001 tttaaagcag gcagattttc cgggccaaaa agatgaccat gcgtcaaaaa cagacgttgt
    12061 ttttccagta atacctgttg ccacggcgcg gttatcggga aatgcagcag catttgatcc
    12121 acttcgctgt cgcagttgcc gcgcacagcg ataaccttat gtgccacttc attaagccgt
    12181 tcagcgactt tggctggcgc gtaaccctcc ggtaaagcat tacgcgggcc gtgattcaac
    12241 acgtcgccaa ggatcaccag ccactgggca ccgctttggg caaacaactc cagaacacgt
    12301 tccgtcgccg gtaacgaccc atgaatgtcc gatgcaaaca tcagtttcat cactcactcc
    12361 tcgtcgaaaa aacgtaagct atgatactgg attctgtcgt tgctatcagc cagattgctt
    12421 cgctgaaagt gcaataaaac gctggacaga cgctcgctgc cactggaatg ttgcataatc
    12481 caccaggcat tctggcacct catcgccatg tagtaccagg cggttaatca ttagcgccag
    12541 atcagtatca gcaatgcacc attcaccaaa taaattcggc tgacccagta ctaacaaatg
    12601 ttctgccatc gcgaacagtt tctctgcact ggcttttccc tcggccgtta gtggcgcttt
    12661 tttcgccccc gcaaagacaa catctgtcgg acgctcttcg cggatgggca tcagatcgct
    12721 gcgcaaccag gcctgaatct gtcgtgcacg cgcacgattt tctaaatcaa gcggataaat
    12781 acgttcccag gtcggtggcg caaatcgatc ttccagatac tccgcaatgg cagaagattc
    12841 actcaactca aaatcatcga tttgtaataa cggcacacgg cgtgtctgac cgtaaccttg
    12901 ccacgtcggt tgcaaatgtt caccgctgtc gaggtcgatg gtcttgatat gaaacaacag
    12961 gcctttttcc tgcaacgcca cccaagcgga taacacataa ggggagaaaa aatgggcatc
    13021 tgaccaaagc gtgatagcgg gtttactcat tatgtcctcg gcaataaagg gggctggcta
    13081 tcaaactata gagccttttt gtcactgtca cgtgacgaat actcacgtaa gtcgccgcgc
    13141 tttctataat tgtggtgttc agatgattag acaaccggag ctccaatgat cgatctctat
    13201 ttcgccccga cacccaatgg ccacaaaatt acgctgtttc tcgaagaagc agggctggat
    13261 tatcgcttga taaaggtaga cctggggaaa ggcggtcagt ttcgcccgga atttttgctc
    13321 atttcgccta acaacaaaat tccggcaatt gttgatcatt ctccagccga tggcggcgaa
    13381 ccgctaagcc tctttgaatc tggtgccatt ttgttgtatc tggctgagaa aacggggctc
    13441 tttttgagtc atgaaacgcg tgaacgcgcc gccacattac agtggttatt ctggcaggta
    13501 ggcggactgg ggccgatgct tgggcaaaat catcatttta atcacgcagc cccccaaacc
    13561 attccttacg ctattgaacg ttatcaggtt gaaactcagc gtctgtacca tgtactgaac
    13621 aagcggctgg aaaactcgcc ctggctggga ggcgagaact acagcattgc ggatattgcc
    13681 tgctggccgt gggttaatgc ctggactcgc cagcgaatag acctcgctat gtatccggca
    13741 gtcaagaact ggcatgagcg gatccgttcg cgccctgcca ccgggcaggc actgctaaaa
    13801 gcacaactcg gtgatgagcg ttcggatagt taacagaaac aggttctcgt gtattatttc
    13861 atcctaagta aaacaacgga gaacctgcaa tggcacaacc tgccgctatt attcgtataa
    13921 agaaccttcg tttacgtaca tttatcggaa ttaaggaaga agaaattaac aaccgtcagg
    13981 atattgttat caatgtgacg atccactacc ccgccgataa agcgcgcact agcgaagata
    14041 tcaacgatgc gctgaattat cgcaccgtaa cgaaaaacat tattcagcat gtagagaata
    14101 accgtttctc tttgctggaa aaattaactc aggatgtgct cgatatcgca cgtgaacatc
    14161 actgggtgac gtatgctgaa gtggagatcg ataaactgca cgcgctgcgc tacgccgatt
    14221 cggtatccat gaccttaagc tggcagcgtt aaccgccata tcgggaggct gcatgaacat
    14281 agtgatcacc ggagggacgg gattaattgg tcgccatttg attccacgtt tgctggagct
    14341 gggccatcaa atcacggtaa tgacgcgtaa cccgcagaaa gccagttccg ttctcggccc
    14401 tcgggtgaca ctatggcaag ggcttgccga tcaaagcaac ctcaacggca ttgatgcggt
    14461 aatcaacctg gccggagaac cgattgctga taaacgttgg actcacgagc aaaaagagcg
    14521 tctctgccaa agccgctgga atatcacgca aaaactggtc gatttgatta atgccagcga
    14581 cacgccaccg tcggtactca tttccggctc ggcaacgggc tattatggcg acttaggtga
    14641 agtggtggtt accgaagagg aaccgccgca taacaaattt acccataaac tctgcgcccg
    14701 ctgggaagaa attgcctgtc gggcgcaaag tgacaaaacg cgagtgtgcc tgctgcgtac
    14761 cggcgtagtg ctggcaccgg atggcggtat tctcggtaaa atgctgccgc cgtttcgtct
    14821 tggcctgggc gggccgattg gttccggtcg gcagtatctg gcctggattc atatcgatga
    14881 tatggtcaac ggcattctct ggctgctgga taacgagctg cgcgggccat ttaatatggt
    14941 ttcgccctac ccgatacgca atgaacaatt tgcccatgcg ctcggtcatg cgctgcatcg
    15001 cccggctatt ttgcgcgtcc ccgcaaccgc cattcggctg ttaatgggcg aatcttcagt
    15061 actggtatta ggtggacaac gcgcgctgcc taaacggctg gaagaagcgg gttttgcgtt
    15121 tcgctggtac gatttagaag aggcgctggc ggatgtcgtt cgctgatgtg gtttactgca
    15181 aacatccgcc agttaactcc cggtgttaca gggttagtgg ctttgcgcga taagatcgtc
    15241 tggtgaaagt cgggtcagcg tcaggatgac ttcgtggtca aatccttttt ccagcatctc
    15301 ctgagcaata cgcagagctt cttcgtgttt gccctgcatt gcgccttctt cacgtaatct
    15361 gtcagcaatg gtcatcagtt tctccttttc ttgtggtgcg cgttctgtta tctcaccaat
    15421 aaatgcacgg aaacgctggg catctcccgt ttgtaataca taattaaaca gggcttttag
    15481 ctgtctgtca ttagtgttcc ctgtaactag cagcgaaaca atttggtcga ctaatcccaa
    15541 cagatcgcgc tgacgaatat gtttctgaat taactccaac agcgccattt tacgatgttg
    15601 cataatctcg tcatccggca ccacggtaat atccaccaac ggaaaagccg atgaatatat
    15661 tttacgggca atcgcaggct cggcaaattc atcaagccag cagagtgaat aaggataagg
    15721 acttctgcaa ccatgataaa acagcatcgg gatcaccaat ggaagctctt tatagcccac
    15781 atcaagatgg ttttgcattg ccgcaatgga ataacgcatc atgcgaaaag ccattaattc
    15841 ttccggctta ctttggtgct ctatcactac ataaagataa cccactccct cctgcgtttt
    15901 cacagaccac aagaggtcgg aataatattg ccgcaggtct tcatcaataa aactgtttgg
    15961 ttccagttta agcgtcgtta aatcacacag tttgcgcagc ggcgcgggaa gatgaatatc
    16021 aataaaatcc cgcgcggtgt ctggatggcg taaaaaagat ttaaataccg catcatgcgg
    16081 cgtggaagtt gtcgatattg tcatggcgtt ccgtcacccc tcaaatttaa gatgacgcga
    16141 caataaccac gattaacacc caatgcagca ggcagttttt atctttggac agcgaattca
    16201 gaagtatttt tagcggactg caacttttac atttcagcgg aagctgactc gcaaattccc
    16261 cttgaaaata ctccggcagc gttagccacc ggagaggcaa ttatttcagc gaacctttaa
    16321 ggaactgttg cagacgaggg ctttgcgggt tgccaaataa ctgctccggc gcgccttctt
    16381 cctctatttt cccctgatgg aggaaaatga cgtgagtaga tacatggcga gcaaagccca
    16441 tttcgtgggt caccaccacc atcgtttttc cctcttccgc cagctgctgc ataatgcgca
    16501 gcacttcacc caccagctct gggtcaaggg ccgatgtcgg ttcatcaaac aacagaactt
    16561 ccggttccat tgccagcgcc cgcgctatgg aaacacgctg ttgctgacca ccggacaggt
    16621 gcaccgggta tttcccctgc gcacgttcgt ctatcccgac ttttgccaga tacttcaccg
    16681 cccgctcgcg cgcttcctgc ttgctcaggc ccaacacctg aatcggcgct tccatgacgt
    16741 tttccagcac cgtcatatgg ctccagagat tgaagtgctg gaataccatc gtcaggcgtg
    16801 tgcgcagtaa gcgcagttga tttttatcgg cgactttgag ttgaccgtct ttgtcgcgca
    16861 ccagattgat cgtctggcca ttgaccacga tcgacccttc actcggtttt tcgaggaagt
    16921 taatgcagcg cagaaaggta cttttccccg atcccgacga tccgatgatg cttattacat
    16981 ctccggcatt cgcttgcagt gacacccctt tcagcacttc gtgttcgcca tagcgtttgt
    17041 gcaaatcgat aacgtttaat ttattctcgg acatcgtatt ctcagtgcgt tgaagaaggt
    17101 ttcacatgct gcaaccagcg tttttccgct ctgcgaaaga ggctgatcag gacataagag
    17161 atgattaaat agagcaccgc ggcaatgccg aaggcggtaa aaggttgata cgtggcggcg
    17221 ttaatatcgc gggctatttt cagcagatcc ggcaccgtgg cagtaaatgc caacgcagta
    17281 gagtgcagca tcaggatcac ttcgttgctg tatgccggta acgcaatacg cagcgcagaa
    17341 ggcaaaataa tgcagcgata cattttaaaa gtcgagaagc cataggctct ggcggcttca
    17401 atttccccat gcggcaccga acggattgcc ccagcaaaaa tctcggtagt gtaagcgcag
    17461 gtgttaagcg tcagcgccag cacggtacag ttcaggccac tgcggaaaaa ggcgttaaga
    17521 aactcggtcc ccttaacaat ctccagcgta tacatgccgg aatagaacac cagcaactga
    17581 acatacagcg gcgtaccgcg aaaaatatag gtaaataacc agattggaaa ctggatgtat
    17641 ttattactgg agacacgacc aatcgccaga aacagcgcca ggactccgcc tatcactacc
    17701 gacaaaataa gcagccacag agtgatcgcc acaccagtaa agcgataacc gtcggtccac
    17761 agcagcggtt tccagtattc atgtaagatt tcgatcacag gtcagccctc ttcacaccca
    17821 cagagtagcg gcgctcaagg aacagcagca caccattgga aacggtggtg aaaaccaggt
    17881 aaatcacgcc acagacgata gcgaaataga acggttccca ggtacttttg cctgccagct
    17941 gcgtggcttt gaccacatct tccaggccga gtaacgaaac cagtgcggta gatttgagga
    18001 tcacctgcca gttgttgcca atgcctggca gggcgtaacg catcatcgcc ggaaacatga
    18061 tccgccgaaa cacttgccca cgagtaaaac cgaacgccct cgccgcctct atatgtcctt
    18121 tcggcactgc cataaaagca ccacggaacg tttcggtaaa ataagcaccg tagataaaac
    18181 cgagagtgat aataccagcg accatcggat cgatatcaat ctgcccgacg cccatcgcct
    18241 ccgtcaccgt gtttagcgca atctgcaaac cgtagaaaat cagcagcatc aacactaagt
    18301 ccggcacgcc acgaatcagc gtggtgtacc cttcgaaaat cagccccgaa agccgatttt
    18361 gcgagagctt accgccagcg ccaattaaac cgatgattac agcgagcact acagagctga
    18421 tagccagctc cagcgtgacg agcgcaccct gtaaaataac acctgaaaac ccatacaaca
    18481 tgctgcctgt cctgtcgtgt gtggtgaatt accgcctctt gtctccctcc ggttacccga
    18541 agggagcggg ctttctttca ccgatggcga ttaaccacca taaacatcaa aatcgaagta
    18601 ctttttcgct aatttctcgt aagtaccgtc agcgcgcatt tcggcaaagg ctttgttcag
    18661 tgcctcgcgc agttcgttat cttctttacg caggcccatg ccggtcccta cgccaaacag
    18721 tttttcatct ttaacagacg ggccaccgaa tttgtaatct ttaccgacag gttgtttgag
    18781 gaaaccttcg ctggcagcga cctcatcctg gaacgcggca tcaatacgtc cggcagtcag
    18841 gtcagaataa atgttgtcct gcccctgata cgagacgatt tcaatgcctt ttggtgccca
    18901 atgttcatta ccgaacgtct cctgggtagt gccctgcaat acgcctaccc gtttgccttt
    18961 cagcgactcg actgtcggct gaatgtcaga atttttcgcc accaccagac gagaatcggc
    19021 agcgtacagt ttgtcggtga aggctatttc ttgctgacgt ttttccgtaa tggaaagcga
    19081 tgacatgatg gcgtcaatct tcttcgcttt taaggacggg attaacgcat ccagcggatt
    19141 ttcgacaaac gtacattgcg tattgatgcg tttgcataat tcctttgcca gatcgatatc
    19201 gaagccaacc agttcgcctt gtgaattttt tgattcaaat ggcgcatagg tcgggtcggt
    19261 accgatgcgg atgttttgcg gaatcgcagc aaatgccgca gttgcgctgg agaaggccag
    19321 aaccagagag agcgatagca ccagtttttt cataatgtcc tcaactgaca gtctttttat
    19381 agggaatatt tacaggtttg ttacacttat cgtgccataa aattgaccat caaggcaaaa
    19441 tatcccgccc cgttagacgt attatttttc attaaaaatg cttaagtatg catttcaatg
    19501 caccacgata gtgcaacaaa cctggcgtgc accatgatga tcattccatc aggtacagct
    19561 tccctgcgac gtatcagtca ccgtagacat taaagtcaaa atactttttc gccatcttgt
    19621 cgtaggtgcc gtcctgacgc agctcgccaa gcgccttatt gaaggcagcc gtcagttcag
    19681 catcatcttt acgtagccct acaccggtgc catcaccgaa gtattttttg tcttttactg
    19741 atgagccagc aaaggcgaaa tctttaccag caggttgctt gaggaatcct tcgctggcag
    19801 caacttcatc ctgcagtgca gcatccagac gtcctgcagc cagatcggaa tagaccaaat
    19861 cctggttggc ataggccacc acatccacac ctttgctacg ccaggtctcg ttagcgtaag
    19921 cttcctgggt tgatccctgc agcacaccaa catgtttacc tttcagtgag tccagcgttg
    19981 gctgaatcgg cgaacctttg gcggcaatca gacgggaatc tgcggcgtac agcttgtcgg
    20041 agaaggcaat ctcctgctga cgtttatcgg taatggaaag cgacgaaata atggcgtcga
    20101 tttttttcgc tttcagtgag gggatcagcg cgtcaaagtc actggcaacc caggtacatt
    20161 tcacctgcat ccgtttgcac atctcgttac cgagatcgat atcaaagccg acaaagtcac
    20221 ctttggcatc tttcgacgag aacggtgcat aggtggtatc ggttccgata cgtaccgtct
    20281 ccggcaatgc cgcatagctg gaagccgctg cggaaatacc gactaacaaa gacagagcga
    20341 gaatcgactt cttcatacat aaccctcaag tgaaaatggc ttttttatgt tgtgtattgt
    20401 gttgtgtgtt tgcaggcttg ttcatgcagg tcttatgcca tcttgacgac aacagtaaca
    20461 ttcaacgtta aatatgttaa taagacgttg cattattgtc ctaaagttga agatagcagg
    20521 tatagcggtt gaatcgcagc gtttcgattg aatggcagaa acaaaatgtc gaggatttga
    20581 tcgcggttgc aaaattgccc tgaaacaggg caacagcgga gttatgcgcc ctgccagcgg
    20641 gcaaagagat cttcaggaag ggttatcgca aactggtcaa gaacacgatt aaccgtctga
    20701 tttatcacat catcaaggga ttgcgggcga tgataaaacg ccggaacggg aggcataatc
    20761 accgcaccga tttctgctgc ctgcgtcatt aaacgcagat ggcctaagtg caatggtgtt
    20821 tcacgcacgc agagcaccag tgggcgacgc tctttcagca ccacatctgc cgcacgggtc
    20881 agtaagccat cagtatagct atggacaatg ccggacaggg ttttgattga acagggtaaa
    20941 atcaccatcc ccagtgtctg gaaagaaccg gaagagatgc tggcggcaat atcgcgcgca
    21001 tcatgcgtga catcagctaa tgcctgcact tcgcgcaggg aaaaatccgt ttcgagggat
    21061 aaggtctggc gcgccgcctg gctcattacc agatgcgttt cgatatctgt gacatcgcgc
    21121 agaacctgta ataagcgcac accataaatc gcgccgctgg caccgctgat gcccacaata
    21181 agtcgtttca taaatagttg ccctgtcaga cttgcaggca gactttgcag gatttcggcg
    21241 ggagttgcaa gtcagggtgc cagaccggca ccctgagcga aggcatcatc cttcgttatg
    21301 catttcgaga ttttccactt cgttctgacg ttgcactgct ttggcgtcgt cattacgtaa
    21361 agtatcgagg aaatcgaggt agccctgatc aacatctttg gtgacgtaga cgccgttgaa
    21421 caccgagcat tcaaactgct ggatatccgg gttttcagcg cgaacggctt cgatcagatc
    21481 gttcagatcc tggaaaatca acccgtcagc accgatgatc tggcgaattt catctacttc
    21541 gcgaccgtga gcgatcagtt ccgtggcgct cggcatatca ataccataaa cgttcgggaa
    21601 gcgaatttcc ggtgccgcag aagcgaggta cactttcttc gctccggctt cgcgtgccat
    21661 ctcgataatc tgctcagaag tggtgccacg gacgatagag tcgtcgacca gcaggacgtt
    21721 tttatcgcgg aactcggcgc ggttggcgtt cagtttacgg cgcacggact tacgacgcag
    21781 ctgctgaccc ggcatgatga aggtgcggcc aacatagcgg tttttaacga agccctggcg
    21841 gtacggtttg cccagaatac gagcaatttc cagcgcgata tcacacgagg tttccgggat
    21901 cgggatcacc acgtcgatat ccagatcttc ccattcgcgg gcaattttct cgcccagctt
    21961 cgtgcccata ttcacacgcg cgctgtaaac ggaaattttg tcgatgaacg agtccgggcg
    22021 agcaaagtat acatactcaa acaggcacgg attgctgacc ggattgtcag cacattgacg
    22081 ggtaaacaac tgcccttctt cagtgatgta aatcgcttcg ccaggcgcga cgtcacgcag
    22141 gaaatcaaag cccagtgtat cgagcgcaac gctttcggaa gcgaccatat attctgtgcg
    22201 gttctcgtca atatcacgtt ttcccagaac cagcggacga ataccgtttg gatcgcggaa
    22261 agcaaccata ccgtggccga taatcatcgc cacacaggca tacgcgccgc ggattaagcg
    22321 gtttgtagca gcgatggcgg cgaaaatgtt gtcggcttca agcgggtagt ggcggaagtt
    22381 gtccagctcg ctggcgaaga tattaagcag aatttccgag tcggaagtgg tgttgatgtg
    22441 gcggcgtttt tcttcaaaca gttttttacg caactcgtga gcgttggtca gattgccgtt
    22501 gtgggcaagc gtaatgccat acggagagtt aacgtaaaac ggctgcgctt cagaggcgct
    22561 ggagctgcca gccgtagggt aacgcacatg accaatgccc atattgccct gcaaacgctg
    22621 catatggcga gcttcaaata catcgctcac cagcccgttc gctttacgca aacggaagca
    22681 gttattggca tctatggtga tgatgccggc ggcatcctga ccgcgatgct gaagcaccgt
    22741 taaggcatca taaatcgact ggttaaccgg cataacaccg gcgataccga caataccgca
    22801 catacgtctt tttcctcgtt aagccacatc tcagagcact tacgctctgg gcaagaaact
    22861 tgacgagctt tgcagataat caaaaaacca tctgatgata aaactgaact gcggaatcag
    22921 ttgtgattta ctccagtctt cgcttttcga caccccggta aaggagtcga gaaagaagag
    22981 aatagcagca acaatcaaca caccgcgcaa tgcaccgaaa cagacgccca gcacccgatc
    23041 ggtgcctgac aaccccgttt tctccaccag caggcctatc acgaagttca cgatagcgcc
    23101 aacgatcagg gtggcgataa acaggaccgc gatggcaatc ccatttcgaa ccagttcgtc
    23161 ttcaaagccc gtaaaccaga ctgacaggta agtgtagtaa tgactggcaa caaagaaagc
    23221 acaaccccat gtcaccagcg ataacgcttc acgaacaaag ccgcggatca ggctaaccag
    23281 agaggaaaaa gcaatcaccg cgattatggc gtaatcaatc cagaccatat gtgtcccacg
    23341 attttacgcc ctgtcatcct gttcggggcg cattctaaca gaaaaagaaa acgtttgcgt
    23401 agggatttcc ttcccgcgca tcaataaaaa tggcgctgaa aaaatattca acgccatcga
    23461 ctttttatgc ctttgcggca tcggacaatg cgtgtcggat gcggcgtaaa cgtcttatcc
    23521 gacctacggt tctacccctg cgtaggcctg ataagacgca ccagcgtcgc atcaggcaac
    23581 ggctgtcgga tgcggcgtga acgccttatc cgacctacgg ttctacccct gcgtaggcct
    23641 gataagacgc accagcgtcg catcaggcaa cgcctgtcgg atgcggcgtg aatgccttat
    23701 ccgacctacg gttctacccc tgcgtaggcc tgataagacg cgccagcgtc gcatcaggca
    23761 acggctgtcg gatgcggcgt aaacgcctta tccgacctac ggttctaccc ctgcgtaggc
    23821 ctgataagac gcgccagcgt cgcatcaggc aagaccgtat taattcggcg tatagcccat
    23881 taccacgcca cttaagccag aaagttgctt caactcaccc agcgaacctt tcagcttatc
    23941 tttcgaggca tccggcccaa ccagaatacg ggtaatttta ccctgcactg gcgtggatgg
    24001 cgacgtataa acccgataac cggcaccgcg cagcttaccg acaatctcat tcactttatc
    24061 ggcatttttc agcgcgccca gttgcacaac ataggcttta cccgttggcg ctgctttttc
    24121 ttccacgacc ggcttcggtt ctggcttcgg cgcaggtggt gcttcaacct tcggcttagg
    24181 cggctccacc ggtttcggct tcggtgggac gaccggtgca ggttccggtt caaactcggt
    24241 gttattggct gcaatagtgg ccggatcgag cgacggtgct gcggcatcac ctgcccgcac
    24301 ctcttccgct gcgccttccg gcggttgcgt cggcaacgcc tgggtggcgg ctggcatcat
    24361 atcaggctca tcgcgatcgc ccgcttttgg caccagcggg atagccgcaa actcatcctg
    24421 ataatgtttt ttctgcccgt ccagcagccc tggaagtaca atcaccccca gcgccaccag
    24481 cacgatcgtg cccactaacc gattctgaaa cttacttgcc accgcttctc ctcgcgtcaa
    24541 tcacttccat gacatgtgcg accgtgtgga acgaaccaca caccagcacg gtgtcttccg
    24601 ctttagcgtc cgccattgcg gcatcccatg cctgcgcaac gctatcaaat gatttgccgt
    24661 tacccaaatg ctcaagcagt tgttctgccg tggcaccgcg cggcccttcc agtggcgcac
    24721 aataccagtc atcaaccacg cttttcaacc aggccagagt tccggcaata tctttatcat
    24781 gtagcatacc gataaccgcc agcacgcgcc cgtttttcgg tagcgttttc atacgcccgg
    24841 tgagatattc cgccgcatgt ggattatgcg cgacatcaaa aataacgcgt ggcgactcgc
    24901 tcacaatctg gaaacgtccc ggcaaaattg cgctggcaat cccgtcgcga atggcatttt
    24961 cactgacctc cagcccgctg gcacgcagtg ccgccagcgc cgttgcggca ttcggttgcg
    25021 ggacaagcgg caacggcagg ttttccagcg tgccgtgagc atcgctaaac gcccagtcat
    25081 gatcggtgac ggaatagttc cactcaacgc cccgacgttg taacagtgca cctttttcct
    25141 gcgccacatc agcaatggta gaaggcattt ccggctcacc gacaattgcc ggtttttcgc
    25201 tgcgaaagat gcctgctttc tcgcgaccaa tactttcgcg atctggcccc agccagtcgg
    25261 tatgatccag cgcaatactg gttactaccg cgacatcggc gtcgacaata ttggttgcgt
    25321 ccagacgacc gcccagccct acttccagaa tcaccacgtc aagttgtgcc tgtttgaaca
    25381 gccacaacgc cgacagcgta ccgtactcga aataggtcag ggaaatatca ccgcgtgccg
    25441 attcaatctc cgcaaaagag gcggtgtggg ccgattccgg caattcctga ccctgcacac
    25501 gtacgcgctc ggtataacac accagatgag gcgaactgta gacgcccact ttgtaccctg
    25561 ccgccatcag aatcgactcc agcgtacggc aggtggtgcc tttgccattc gtacccgcaa
    25621 cggtaaacac aaatggcgct ggtttcagaa cgccaagacg cgccgcgacc tggctcacgc
    25681 gctcaaggcc gagatcgata gttttactgt gcaggttttc cagataagaa agccacgaag
    25741 ccagaggcga cgcggcttga ggagtgcgtt tgataatcat ggtatccgct gattcgttac
    25801 ggtgagaata gcaaaagggc agagccagtg gccctgccct tatcagttat caggcctcag
    25861 gttcctgatc cggtaccggg ggtaccacta cgccttcacg cggcgcttca ggattcggcg
    25921 ctggcagatt catcaacttc gccagaatgc tcgccagttt caggcgcatt tccggacgac
    25981 ggacgatcat gtcgatcgcg cctttctcga tcaggaattc actgcgctgg aatcctggcg
    26041 gcagtttttc gcgaacggtc tgttcgataa cacgcggacc ggcaaagccg attaacgctt
    26101 tcggttcagc gatgttgaga tcgcccagca tggcgaaact tgcagaaaca ccgcccatcg
    26161 tcgggtcggt cagcacggag atgtacggca agccgcgctc ctgcattttt gccagtgccg
    26221 cagaggtttt cgccatctgc atcagcgaca tcagtgcttc ctgcatacgt gcgccaccag
    26281 aggcggagaa gcagatcagc gggcagttat cttccagcgc ctgctcaacg gcacgcacga
    26341 aacgtgcacc cacaacagac cccattgaac cgcccataaa ggcgaactcg aatgccgcag
    26401 cgacaaccgg cattccatac agagtgcctt tcatcaccac cagcgcatct ttttcgccgg
    26461 tttctttctg cgcagatgcc agacggtctt tatacttctt ggagtcacga aacttcagca
    26521 catctttcgg ctcaagctcg ctacccagct ccacaaggct tccttcatct aacaggctat
    26581 gcaggcgatt acgcgctgtc atacgcatgt gatggtcaca cttcggacag acctcaagat
    26641 tacgttccag ctcagcgcgg tataaaacct gaccgcagct atcacactta gtccacaccc
    26701 cttcaggaat gctcgccttg cgggtgggag taatgttgct tttaattcgt tcaatccagc
    26761 tcattaggga cctttctgtc tgaacctggt tcgatgccag ttttatcttt ggggacgcat
    26821 aatgccattt ttgcccccaa cagaccatga atgttgcaca ttaaaacata acagcccgaa
    26881 actttggata aaaaagtggt cgaaccgcgg agttactttt tattttgcgg cgcgtgccgc
    26941 agcgcgtttg tgacggatta tttcgatgac gcccggcaaa atagaaacca caataatccc
    27001 gacgatcagc agcttaaggt tatcctgaac catcgggatt gtaccgaaga aatagcctgc
    27061 gtaggtaaaa agcagtaccc acaacagtgc gccgatcacg ttataagcag cgaaatgacg
    27121 gtacgacatg tggcccattc ccgcaacaaa cggggcgaac gttctgacga tcggcacaaa
    27181 acgggcgaga ataatcgttt tgccgccatg tttctcataa aactgatggg ttttatcgag
    27241 ataactgcga cggaaaattt tcgaattggg attactgaat aacttttcac cgaacaagcg
    27301 cccgatagtg taattgaccg cgtcacccac aatcgcggcg atcagcatca gcaccaccat
    27361 catatggacg ttgagatcgt tggtttccag tgatgccaac gctccggcaa caaacagcaa
    27421 tgaatcaccc ggtaaaaacg gcgttaccac cagaccggtt tcacagaaca aaattaagaa
    27481 caaaatggca taaacccaga cgccgtactc cgcgaccagc tctgccagat gcacgtcaat
    27541 gtgcaggata aaatcgatga ggaaataaat caggtccata tttgcctatg ccttgtactc
    27601 gtcatctttc aggctgtaac tacgttggct acagcctgaa atgttccgag cattattctc
    27661 gttagtccgc cagaaatagc gggcccattg gcggttttgg aagatcatac cggtcagggt
    27721 aatccaccgc gaccagatac agcccttccg cttttgccgt tgctgccgcc agcgttctgt
    27781 cctttgccgc cagcaactct gctatccagc tctccggctg gttgtgggca ccgacttcca
    27841 tcaggctgcc gacaatattc ctgaccatat gatgtacaaa ggcattcgct ttgatatcaa
    27901 ccaccacata aggaccgtga cgcgtgacat taatatgcat aacgttgcgc cacggggttc
    27961 gggactggca ctgcaccgca cggaacgagg tgaaatcatt ctcgcccagc aagcactgcg
    28021 cagcccgatg catccgttca gcgtccagcg gttcgtaaaa atgggttacc cctttactca
    28081 gtaccgccgg gcgcagccga tgattgtaga tgatgtagcg ataacggcga gccgtggcgc
    28141 taaatcgggc atgaaaatca tcaggtacag ctttaaccca acgcacggcg atgtcaccag
    28201 gtaaattcgc atttacgccc aacgtccacg cagcgtcttt gcgctgcgcg gtagtttcga
    28261 aatgcacaac ctgcccggta ccgtgtaccc ctgcgtcagt acgcccggcg cagaagacgg
    28321 tgatgggttc gttcgccacc tgggagagcg ccttttccag cttctcctgc acactgcgga
    28381 cttcattctg ccgttgccag ccgtaatact tactgccgtc gtactcaatg cccagcgcaa
    28441 ttttataaac tggcggttgt tgctggtcgg acattagtac agatactcct gcaccagttt
    28501 ctcggcgatt tttactgcca tcagcgcgcc gccaaagcga acgttatcgg ccaccgacca
    28561 gaactggact tgctccggca taccgtagtc attacgcacg cagccaacag aaagatgcgg
    28621 cgtacccgaa gcatcaccta cctgagttgg gaattcgttc tcttcagaga gcacaatatc
    28681 ttccccttga acaaacgcat cacgcgcttc ttctgctgcc agtggacgca gagcttcaaa
    28741 gttgaccatc tgggcatgac cgtagaatac cggtgcctgg acgacgcttg ccgaaatcat
    28801 cagtccttcg tcctgcagga ttttgcgtac ttcgtcaacg atacgacgtt cttcacgcac
    28861 gctaccttcg ctatccggca gtaacggcag catgttgaac gcgagctgac gcccgaagaa
    28921 atcttcttcg tcaatcggaa tgccgttgag caatttcgca ctctgccccg ctaacgcatc
    28981 gaccgctttt ttgccctggg cggaggctga aatcaggctg gtaacactga tacgcgacaa
    29041 accgccctga tcgattaacg gtttcagtgc cgccagcagc tggctggtca gactgtctgg
    29101 tacggcgatg acattccggt tgcggtaatc ggtcagtaca aacgggttta cttccggcac
    29161 caccagcggt acgtcgggtt caagagcaaa caatccgctg ctgtcgatca ccaggcaacc
    29221 tgagttggtc gcttcttcaa cccaggctgc ggtagcttct ttgcctgcga caaaaaatgc
    29281 cagctgcgcc tgcgtccagt cgaattcagc agcatcctgc acggtgattg tcttaccacc
    29341 aaagcgcaat tgttcgcctg cgctttcgtt acgtgccagt gcataaattt ccccaaccgg
    29401 gaactgacgt tcagccagcg tttcaagcag ggcttcgccc acagcgccag ttgtgcccag
    29461 gacggcaatg ttccagcctt cagacatggt ggtttactcc agaaatagca aagctccctg
    29521 ccaatgttat agcagagagc atgaagaaga gattaacgtg ccggatgatg aacggcgtta
    29581 aaacccagtt tacacagcaa tgatgccgca ctggcgtcat cacaaattac atacagagat
    29641 gaccattcac ggcgctcaag atagtttttg cgcagtttat cgaactcacc cggtatcccg
    29701 gcgactttac gcagcggtgc gtcatcgcgg cgcacatcat acaccaaatg caccagcctt
    29761 ttcagcgtcg gttgatcgag cggtccatgc agagtaatgc gaccaaactc tggcgcaggc
    29821 agtaatgtat ccagcgcaac gtgctgttca tgcccaataa acttgctata ggcttcaaac
    29881 acttgcgtag taccgcgtgc tttaccttcc agggtatagc ctgcaatatg cggcgtgccg
    29941 atatccactt ttgtcagcaa ttccacattt agttccggtt cgccttccca gacatccagc
    30001 actacgctta acttctggcc ttcattcagg caggtcagca gcgcggtatt atcgacgact
    30061 gcgccacggc aggcgttaat cagaatcgct ccgggcttca ggctacggat cagtttttca
    30121 tccgccagat gcagcgtttt gtacggcccg tctttgaaga gtggcgtatg gaaagtcaga
    30181 atatccgcgc gctggactaa ctcatccagc gagcggaaat caccctcatc cccacagtcg
    30241 gcgcgaggcg gatcgcaaag taaggtttta atccctaacg cttccagtcg tgcctgtaaa
    30301 cgccgaccaa cgttaccaac gcctacgatc cccacggtac gctcgtgcag tgaaaatcca
    30361 tcgcgttcgg caagcatcag cagggaggag aaaacatatt ccaccaccgc aatcgcatta
    30421 cagccaggtg cagcggaaaa accaattccc gcctgcttca accatgcttc atcgacatgg
    30481 tcggtccccg cagtggcagt gccaacaaat ttaatgggtt ttcctgccag caaagattca
    30541 ttcactttcg tgaccgaacg caccatcagc gcatccgcgt ctgccagttg agcgacgggg
    30601 attggacgcc cgggaaccgc gatcacctca cccaaacggc taaataagtc gcgggcataa
    30661 ggcatatttt catcaacaag gattttcacg tttgtgttac ctgtatgaga cgagagttaa
    30721 ccggacaagt gtgccataat ctcgcggcca ggcatacttg cgaagatttc aggtataagg
    30781 atacgtaatg atacaaccta tttccggccc tcctcctggg caaccaccag gtcagggaga
    30841 taacctgccg tctggcgcgg gcaatcagcc tttatccagt cagcaacgta cttcgctgga
    30901 aagcttaatg acgaaagtga cctcactgac gcaacagcaa agagcagaac tgtgggcggg
    30961 tatcaggcac gatattggtc tgtcgggaga ttcaccgctg ctttcgcgtc actgccctgc
    31021 cgctgagcat aatctggcgc aacgtctgct ggccgcgcaa aaaagccatt ctgcccgcca
    31081 gcttttagcg caattagggg agtatttacg tctggggaat aatcgtcagg cggtcacgga
    31141 ttatatccgt cataactttg gtcagacgcc gctgaatcag ctctcaccgg agcaattaaa
    31201 aaccattctc accctgttgc aggaagggaa gatggttatt ccgcaaccgc agcagagcga
    31261 ggcgaccgat cgtcctctat taccggcgga gcacaatgcg ctcaaacagc tggtgaccaa
    31321 acttgcggcg gcaacggggg agcccagcaa acagatctgg caatcgatgc tggaactttc
    31381 cggggtgaaa gatggcgagt taattccagc gaaactgttt aaccatctgg tgacctggct
    31441 gcaggcgcgt cagacgctaa gccagcaaaa tacgccgacg ctggaatcac tacaaatgac
    31501 gctaaaacaa cctttagatg ccagtgaact ggcggcgtta tcggcatata tccagcaaaa
    31561 atatggcctt tctgcgcaat catcgctttc ttctgcccag gccgaggata ttcttaatca
    31621 gctttatcaa cggcgggtta aagggattga tccgcgtgat atgcaaccgc tgcttaatcc
    31681 ttttccaccg atgatggaca cgttgcaaaa tatggcaacg cgtcccgcgc tgtggatact
    31741 gttagtcgcg attatcctga tgctggtctg gctggttcgt taaccccgac gaaacgacag
    31801 tatcgtgaca ataattccca gcaccgcaga gatcgccccg gcaagaaata ccgaagagta
    31861 accaaacgtg gtcgccagca ttcccgcaag cggcccggag acgccgaggg cgatatcctg
    31921 aaacgcggcg taaccgccca gtgcggtgcc gcgaacttgt gaggggacgc gtttaaccac
    31981 ctccacaccc agcgcaggaa agataagcga acatccggct ccggttaacg ccgcgcccgc
    32041 taatgcgacc cacgcccctg gggcttgcca gagcagcaac aagcccaccg tttctacaag
    32101 cagagagaca atcgccactt tcacgccgcc aaaacggtcc ggcatccagc caaacatgac
    32161 gcgcatcacg acaaatgcgc cgccaaacgc ggtaagagta aagcccgcca tcgcccatcc
    32221 tttgctggca aagtagagcg aaacgaaagt cccgataacc gcaaaaccaa cgccttgtag
    32281 tgccagacct aaccctggtt tccagataag tccgacaacg ctccacagag atggacgttc
    32341 tcccgccagg gctggtactt tgcgcactgt gccgttacag gcccacgcca gtaagggtaa
    32401 tgccattgtg gtgagcgcca gtgcggcaaa accgtaatgg ctatgaatca acaggccaag
    32461 cggagcacca acagcgaggg caccgtaaat cgccattcca ttccatgaca tcactttgcc
    32521 agagtgtttt ggccctacga tgcctaaccc ccaggtcaga gcgcctgtca gtaactggct
    32581 ttcaccaaac ccaagaatca aacgcccgat gaccaacagg gcaaatttga acggtgcgga
    32641 gacaggcaaa atcgccgcca gcagcaacgc gccgccagcc agaccacaag ctaacatccc
    32701 ctgaagcgcc gaacgttttg cgccatattg atcggccagt cgcccggcat agccacgcgt
    32761 cagcaccgta gccagaaact gaatgccgac ggcaatgccg accatggtat tgccatagcc
    32821 cagatcatga tgaacaaaca acgggataac cggcaacggc aaccctacgg tcatgtaggt
    32881 gagaaaaacc gcaaaagcga tgcggaagag cgaaaaattg gcagaagatc gtgtttcggt
    32941 ttggcttaca gcagtcatgc attactccag aatgcagcgc aaggcgagga gtatccccgt
    33001 ctcatctctc tggtttcagg gttacagtgc gttggcagga tttaacgcgt acgtcttttc
    33061 agaaggaaat cgacaaagcg gggagtttgc ctggaactgg cggcgattgt caatgatgtg
    33121 aaaaagggaa ccatcaggtt cccttttgcg ttagtgccgg aggccgcatt ggcgcgtaac
    33181 gtcggatgcg acgctggcgc gtcttatccg acctactccg gcctactgcg aattaatctt
    33241 tcagcttgcg cattaccagc gtggcgttgg tgccgccgaa gccgaagctg ttagacataa
    33301 cagtggtcag ttcgcgatcg gtcgtttcgg tcacgatgtt cagacccgca gcctgctcgt
    33361 ccagctcttc aatgttgatg ctcggggcga taaagccgtg ttccagcatc agcagagagt
    33421 agatagcttc ctgtacgcca gcagcgccca gagagtgacc ggtcatggct ttggttgcag
    33481 aaatcgccgg gctcttatcg ccgaacactt cacggatagc tgccagctct ttcacgtcgc
    33541 caaccggagt cgaagtgccg tgggagttca ggtaatcgat tggggtatca acgccatgca
    33601 tcgccatctg catacaacgt actgcgcctt cgccagaggg agcaaccatg tctgcgccat
    33661 cagacgttgc gccgtagcca acgatttcag catagatgtg agcaccacgc gccagcgcgt
    33721 gttccagctc ttcaaccacc accataccgc cgccgccagc gataacgaaa ccgtcacggt
    33781 gagcatcgta agtacgggag gctttttccg gggtgtcgtt gtatttagta gacagcgcac
    33841 ccattgcgtc gaattcgcaa gccatttccc agcacagctc ttcgccgccg ccagcaaaca
    33901 cgatgtcctg tttgcccagt tggatctgct ctactgcgtt accgatacag tgtgcggaag
    33961 tcgcacacgc ggagctgatg gagtagttaa cgccatgaat tttaaacggc gtggcgaggc
    34021 aggcagaaac gccggatgcc atcgctttgg tgaccacata cgggccaacc gctttcaggc
    34081 cgcgcgggcc gcgcattgcg tcagcgccga acacctggaa acgcggggaa ccgccgccag
    34141 aacctgcaat caggccaaca cgcggattat tctggtaagc ttccggagag aggcccgcat
    34201 ctgcgattgc ctgctccata gaaaggaatg cataaatgga tgcgtcgctc ataaagcgca
    34261 caactttgcg gtcaatgagg ccagtggtat ccagttttac gttgccccag acgtggctac
    34321 gcatgccgga atccttcagc tcctgagaga aagtgatccc tgaacgtcct tcacgcagag
    34381 atgccaggac ttcctgctgg ttattaccga tgctggaaac aatgcccagg ccagtaatca
    34441 ctgcacgttt cattcaatac ctctgtaagt cgcacataga gtaagtttcg aatgcacaat
    34501 agcgtacact tgtacgccga acaagtccga tcagccaatt aatagagaaa tttgcgcagc
    34561 cttacacaca tcgctaagat cgagctaccg cctgtaagac gagtaactta cgtgaaacac
    34621 tactccatac aacctgccaa cctcgaattt aatgctgagg gtacacctgt ttcccgagat
    34681 tttgacgatg tctatttttc caacgataac gggctggaag agacgcgtta tgtttttctg
    34741 ggaggtaacc aattagaggc acgctttcct gaacatccac atcctctgtt tgtggtagca
    34801 gagagcggct tcggcaccgg attaaacttc ctgacgctat ggcaggcatt tgatcagttt
    34861 cgcgaagcgc atccgcaagc gcaattacaa cgcttacatt tcattagttt tgagaaattt
    34921 cccctcaccc gtgcggattt agccttagcg catcaacact ggccggaact ggctccgtgg
    34981 gcagaacaac ttcaggcgca gtggccaatg cccttgcccg gttgccatcg tttattactt
    35041 gatgaaggcc gcgtgacgct ggatttatgg tttggcgata ttaatgaact gatcagccaa
    35101 ctggacgatt ctctaaatca aaaagtagat gcctggtttc tggacggctt tgcgccagcg
    35161 aaaaacccgg atatgtggac gcaaaatctg tttaacgcca tggcaaggtt ggcgcgtcca
    35221 ggcggcacgc tggcgacatt tacgtctgcc ggttttgtcc gccgcggttt gcaggaagcc
    35281 ggattcacga tgcaaaaacg taagggcttt ggacgcaaac gggaaatgct ttgtggggtg
    35341 atggaacaga cattaccact cccctgctcc acgccgtggt ttaatcgtac gggcagcagc
    35401 aaacgggaag tagcgattat cggcggtggt attgccagcg cattgttgtc gctggcgcta
    35461 ttgcgtcgcg gctggcaagt aacgctttat tgcgcggatg aagcccccgc gctgggtgct
    35521 tccggcaatc gccagggggc gttgtatccg ttattaagca aacacgatga ggcgctaaac
    35581 cgctttttct ctaatgggtt tacttttgct cgtcgacttt atgattcatt acccgttaaa
    35641 tttgatcatg actggtgcgg cgtcacgcag ttaggctggg atgagaaaag ccagcataaa
    35701 atcgcacaga tgttgtcaat ggatttaccc gaagaactgg ctgtagccgt tgaggcaaat
    35761 gcggttgaac aaattacggg cgttacgaca aattgcagcg gtattactta tccgcaaggt
    35821 ggctggctgt gtccggcaga actgacccgt aatgtgctgg aactggcgca acagcagggt
    35881 ttgcagattt attatcaata tcagttacag gatttatccc gtaaggatga ctgttggttg
    35941 ttgacgtttg caggagatca gcaagcgaca cacagtgtag tggtactggc gaacgggcat
    36001 caaatcagcc gtttcagcca aacatcgtct ctgccagtgt attcggttgc cggtcaggtc
    36061 agccatattc cgacgacacc cgaactggca aaactgaaac aggtgctgtg ctatgacggt
    36121 tatctcacgc cgcaaaatcc ggcgaatcag caccattgta ttggtgccag ttatcatcgc
    36181 ggcagcgaag agacggcgta cagtgaagac aatcagcagc agaatcgcca gcgattgatt
    36241 gattgtttcc cacacgcaca atgggcgaag acggttgatg tcagtaaaaa agaggcgcgc
    36301 tgcggtgtgc gttgtgccac ccgcgatcat ctgccaatgg taggtaatgt tcccgattat
    36361 gaggcaacac tcgtggaata tgcgtcgctg gcggagcaga aagataaggc ggtaagcgcg
    36421 ccggtttttg acgatctctt tatgtttgct gctttaggtt cgcgcggttt gtgttctgcc
    36481 ccgctgtgtg ccgagattct ggcggcgcag atgagcgacg aaccgattcc gatggatgcc
    36541 agtacgctgg cggcgttaaa tccgaatcgg ttatgggtgc ggaaattgtt gaaaggtaaa
    36601 gcggttaagg ccgggtaatc tgctctcgca gtgtttgccg gatgcggcgt gaacgcctta
    36661 tccgacctac gtgtgacgtg taggcctgat aagacgcgat aagcgtcgca tcaggcatgg
    36721 tgctccagac gccgcaactt actgctgtga cgcctgctga aacaggtttt cccacatatc
    36781 ggtcaccagc gcctggtcac gcggcgacaa ttcaccagca ccaatggctt tttccagact
    36841 ctggctaacg gtcgtatgca ccgcctgagc ggagtggtca tcaccacttt ccagttctgc
    36901 gatggctaat gtaaggtggc cacgcaaata cccactggca aacaactcat catcactggc
    36961 atggtcaacc ataccgtcga ttaatgccag aatgcgtgat tcaaactccg cgatcatctt
    37021 ctttcctcat tgtaaaacgt ggcgctggct tcagttgagc gcttccggcc acgggaactg
    37081 ttccgccgta agttccggcg tgtgataata attttgtagt gctttaatga aacgtgccgg
    37141 gcgttccgga atgccatttg ttagataatc catcacctgc gcatgtactc gccgttgaaa
    37201 caccacgcgg tccggttcga agtcgccttc cagattgtcg caactgacgt taaacgggta
    37261 tcccgccgcc acgcagaaca accagtcgag cgcctgcggc ttcacttcaa catcttcaaa
    37321 ctggctttgc gtttgggcat cgcgaccgtc cgggcaatac cagtagccga aatcaacctg
    37381 ctcgcggcgc gctttcccgg caatacacca gtgcgaaatc tcatgaattg cgctggcata
    37441 aaagccgtgg gcaaagacga ttcggttata cggtacttcc gcatcagcgg gaagatagat
    37501 cggttcgtcg tcgcctttaa tcagacgggt attaaaatca tcggcaaagc agctattaaa
    37561 aatttcaatc aactgctcat agtggtgtgt actgttcatt agttcatccc caaccagtgg
    37621 aggatctcct gtccgtggct atcataaagt aatttggcac tcatcaccgc cgagacgata
    37681 acgatcatcg ggcggatcag tttttgtcct ttgctcaaca ccagtcttga acccatgcgc
    37741 gcgccaagga actgccccac cagcatcaca aagcccgtcg cccaaatcac tttgccgccg
    37801 agaataaaca gcagtaaacc gccgatgttt gacgttgcgt tgagtaattt ggcgtgagcc
    37861 gtggctttgg cgaggttgaa tccgcacagc gtaacaaagg ccagcgcgta aaacgatccg
    37921 gctgccggac caaagaatcc gtcataaaaa ccgacgcagc caccggcaat taacgcgaag
    37981 ggcaaaccgt acatccggcg ctggcgatct tcttcaccca gctttggcat cagcaagaaa
    38041 tagaggccga tacaaatcac cagaatgggc aaaatctgcc gtaagacatc agcctgaacg
    38101 tactgtacca acagtgcgcc gctcattgag ccgacaaagg tcatggcaat attaagtttc
    38161 tgatcgctta aactaaccac tttgcggcga ataaagtaga tagtggcgga aatagagccg
    38221 ccgcaggctt gcagtttatt ggttgccaga gcattagcgg gagacatccc cgctgccatc
    38281 aatgccggaa tggtgagtaa cccaccgcca ccggcaatcg agtcgataaa tcccgccagc
    38341 atggcgacaa aaaagagaac tcccagcaac agcggggaaa ccataaacag gctattaaac
    38401 gtttccatta gatcacgtgc tcatccagta gcgcctggca ggaaggcggc aacggaggcg
    38461 gtgtcttctt ctcaggcttt gttgttcccg gttttggagg ttcaaaccag ctttgcagtt
    38521 ctgccccgca accatcgcct ggtggcggta aaggttgatc ttcacactcc agactatcgg
    38581 caggacaacg taatcgtaca tgcatatgcg cacgatgctg gaaccagggt cgcactttgc
    38641 gcaaccagtc gcgatcggtg cccgcatcaa ggcaaagttg ttgtttaatc gccggattaa
    38701 caaaaatgcg cgtgacgtct ttgtcctggg cggcgagttt gatcaagctg aaaatttctg
    38761 gcttccacag cgtggagaca acgtgtttac cgtcgcggga aactaagtcc agtgcttgcg
    38821 ggcgcaagag ctgcgcggag gtccggcgtg ttttcggcag ttgcagaaag atatcgacat
    38881 ccagtccggt ctggtggctg gcgtgaccgc cgttgaaacg cccaccagca ggcattccca
    38941 tatcgccaat caacaccgta cccatgccca gattgctcac ctgactactc aggcgctgga
    39001 taaacatcac caggtccggg tgaccgaaat agcgacgctg atcggtacgc atgacctgat
    39061 aatgttcgga ctgtatcggc aacgtgtcag cgccgacgat acaaccattg gaaaaactgc
    39121 ctatcgattg tgcgctaccc ggcacaggtt gggttatttt ttgccacggc gtcgctgcca
    39181 ggctgacgct actggcaagc agggccagca gcgcaatcgc ggttttgttc attttttacc
    39241 agcgtggaat atcagtcttc acatcggcat tttgcgcccg ctgccgtaac aggtgatcca
    39301 ttaaaacgat cgccagcatc gcttctgcta tcggcaccgc gcggatcccg acacagggat
    39361 cgtgacggcc tttggtgatc atctcaactt cttcgccaaa gcggttaatg gtgcgccccg
    39421 gcacggtaat actggaggtc ggtttcagtg ccatatgagc aatgatttgc tgcccgctgc
    39481 taattccgcc gagaatgccg cccgcatggt tactctggaa accgtctttg gtgatttcgt
    39541 cgcggttctg gctgccacgc agcgccacca cgtcaaaacc atcaccaatt tccacgcctt
    39601 tcaccgcgtt aatgctcatt agcgcatggg cgatgtcggc atccaggcga tcaaagaccg
    39661 gctcaccaag cccggcagga acgccactgg caacaacggt gactttcgcg ccaatagagt
    39721 cgccctcttt tttcagcgcg cgcatcagtt catctaacgc gtcgattttg tccgggtccg
    39781 ggcagaagaa tggattttgc tcgacctgcg accagtcttt gatttccagc ggaatgtcgc
    39841 ccatctgggt caggcagccg cgaatttcga tgccaaattt ctcagcgaga tattttttgg
    39901 caatcgcccc tgctgcaacg cgcatggcgg tttcacgggc ggaagaacgt ccaccgccgc
    39961 gataatcgcg cagaccgtat ttttgttcgt aggtgtaatc ggcatggcca ggacggaaaa
    40021 cgtccttaat cgcactataa tcctgagaac gctgatcggt attttcgatc aacaaaccaa
    40081 tgctggtgcc ggtagtaaca ccttcaaaaa caccggagag aattttgacc tgatctggct
    40141 cacgacgctg ggtggtatag cgcgatgtcc cagggcgacg acggtcgagg tcatgttgca
    40201 ggtccgcttc cgtcagcgga atgcctggcg gaacaccatc gacgatgcag ccgagcgcca
    40261 gcccgtgcga ttcgccgaag gtggttacgc gaaagagttg tccaattgtg tttccagcca
    40321 tcacggctcc gttattgttg tgtttgcgtg tttacttaat ctttataaat cgcgaaatgt
    40381 tcacgggccg ccagcagctg cgctttggtg agcataaaca caccatcgcc gccgttatca
    40441 aactccagcc aggtgaacgg aacatcggga tattgttcca taagatgtac catgctgttg
    40501 ccgacttcac aaatcagtac gccatcatca gcaagataat ctgccgcgtt tccgagaatg
    40561 cgacgcgtca gtttcaggcc gtcggtgcca gatgccaggc ccagttccgg ctcgtggcgg
    40621 tattcatttg gcaggtcgga catatcttcc gcatcgacat acggcgggtt agtgacaatc
    40681 aggtcgtact gcactttcgg caaatcacgg aacagatcgg aacgaatcgg tatgacgttg
    40741 tggatcagac cgtgttcttc gatgttctgt tcggcaaccg ccagcgcgtc gggagagata
    40801 tctaccgcat cgacttccgc ttccgggaag gcataagcac aggcaatggc gatgcagccg
    40861 ctaccggtac acatatctaa aatatgctgc ggttgcttgc tgataagtcc ggcaaattta
    40921 ttgttgatca attcaccaat cggcgagcgc ggcaccagca cgcgttcatc gacgtaaaat
    40981 tcatggccgc agaaccacgc tttgttggtc aggtaagcta ccggaatgcg ttcgttgacg
    41041 cggcggatca cgcgctcaac aatacgatgt ttttcgctgg aggtcagacg cgcggtgcgc
    41101 atatcttccg gaatatccag cggcaggtag agcgacggca ataccagctg tacggcttca
    41161 tcccacgggt tatcggtgcc gtgaccgtac cagatatttg ccgcgctgaa gcggctcacc
    41221 gaccagcgca acatgtcctg aatggtttgc agctcattta ctgcttcatc aacgaaaatt
    41281 ttatccacgt attcctccag ggcatgatcg caataatttc ggcggctagt ttgccatgaa
    41341 gatgacgata aatcagcatt cacgcgcggt gagtgaggaa aaatacgttt aaaacgatcg
    41401 attgcgctac gagtcgggta aactgtagga aaattagaaa tagagacaga taaatgaaaa
    41461 agaaaacaac acttagcgag gaggaccagg ctctgtttcg ccagttgatg gcggggactc
    41521 gcaagattaa gcaggacacc attgtccatc ggccacagcg taaaaaagtc agcgaagtgc
    41581 ccgtcaaacg gttgattcag gagcaggttg acgccagtca ctatttctct gacgaatttc
    41641 aaccgttatt aaatgctgat ggcccggtga agtatgtacg ccctggtgta gaccattttg
    41701 aggcgaaaaa gctgcgccgc ggcgattatt cgccggaatt gtttttggat ttacacggtc
    41761 tgacgcagct gcaggccaag caggaactgg gcgcgttgat cgccgcctgt cgccgcgaac
    41821 atgtgttttg cgcctgcgtg atgcatggtc acgggaaaca tatattgaag caacaaacac
    41881 cgctgtggct ggcacaacat ccgcatgtca tggcgtttca ccaggcacca aaagagtatg
    41941 gtggtgatgc cgcattgctg gtgttgattg aagtggaaga gtggctaccg ccggagttgc
    42001 catgaatatt agggggcgga tgcgttttgc catttcgccc tgaatgctgg tatcgaatgc
    42061 cggatgcggc gtaaacgcct gatccggctt atcaaaatgt gcaaattcaa tacgttgtga
    42121 ttcagttgta ggcatgataa gacgcgtcag cgtcgcatca ggcatcaacg cacaactaca
    42181 gttcattcag tacatactgg aaacggcatg tagaatcaat tggctcacgg ctactggtat
    42241 tcacctgtac cacaatgtcg tgtagctttc tatccgtcaa tggttgacgt gagggggtga
    42301 ctgtcacctt tgcattatca caaccatacg aggtaaaggt taataagtca gaaacagcac
    42361 gacaatatgc ccctctggaa tcaaaaatac tgccatcgca gagtttgccg ctttccatca
    42421 ggtaggtcga aagcgtagtc gtgattgtcg aaccatcgga ttgcaatcgc agcgctttat
    42481 tatggctaac ataattatca ctccaggata tatcaaacga agcgacggga attgtcgggc
    42541 aaatagaatc atgaactgtt gggcttgtat acgtaccaaa tccaaatgcc tcagatactg
    42601 aataaaatga ggcaatccac ggacaaccat aattatcaga ttctacagtg acgttaaaag
    42661 tcatatatgc acagacaaag acacgagtat tgataccagg gcaatttgag gtgtccgtac
    42721 gtctataatc agtcaaaacc agtgtcggtg ccgggccaga tccgctccag gatattaact
    42781 tgagggtgtc gccttttctt gcattggagt tgtattgtcc gtagcttact tctcccggcg
    42841 tcactaccgt gacatagaaa tataaactgc cggaagcagt aacgtttttc atcgcataat
    42901 aaattttagt actggaaaag cttgcactca tgagtttatc accggcatag cactttggtg
    42961 tttctaaact gagagcaagt aatatcagac ttgtcaaaaa tatcgtccat tttttcattt
    43021 taaaaatact caaaagttac cgtcattgtg gcggtgaaat cacccgatgt aacatttcgt
    43081 ccatttacgg tctgtaacca ggcattaaaa ttgacactgt tatttccctg atttaatgga
    43141 aatgaggcac ctgtggtact gttaataggt acagccgccc ctccggcagt ttcaatgcca
    43201 atcccaacac ccgttgcaga agatgacgaa tcaatcgaca gaaatcctgg taagtcggta
    43261 tcttctgttc ccatcaaggt caccttgaca ttaaacgccg tggtgctttt gcaatctttc
    43321 aactgaaaaa ctaacggtac gcgatctgac tgaccacgtt gcattaaatc gctggcagca
    43381 attgtgggga aatgaatttc tgcaagtaag ttgccattga ttacaggagt acaggattta
    43441 ccaagcaaat taccggtaaa atgcaggtta tcggcagctg gggccgttcc gctgaacaat
    43501 atcagaccac tgataagcgc ccacgcacgg ttattcttca ttggtaatcc acctgtaacg
    43561 tagcccatgc ttcaaagtct gactcgggta agtcgacacc gctctttttt acgggtactg
    43621 ccgttagtac cggaagggat gtttcattca ccactaaggg cgtatgcagc ttaaaatcgc
    43681 tcccggcttg tttgaggtgt attcctaatc cattgagatc cgtggctacg gccgaatcgt
    43741 caaagtcact ctgcgtacca ctccaggtca acgtcatttc catttgaggg tcacggacag
    43801 cggtatcgca ggtaatggta tacggcacga cctgttcgta attgctaccg ttaatgtcat
    43861 caatattcac gtcccgaaac gtcaactcta tattttgccc attactaata gagcaattgg
    43921 gtggctgcac taaataacca tgaaaagaga cattcgagag cgccataaag ctaaagctcc
    43981 acaacattaa gagagagatc cgtttcattg ataatccacc acaattgtgg cactggcgct
    44041 aaattccgcc gccgttagcg atacaccgct ctgcacgaca ggaaccgctt ccagagccag
    44101 tgcgccagat gaaaagttaa acggtaacca tgcgccaggc gttaaatcca aaatggagtg
    44161 atcgcttact ttctgaatac ggataccaaa accggcaata cccgtatcga ttaccgtttc
    44221 tccgttaatt acggttgtgg ttgcctgcat atttaagcgc aggtcgtcca ttgccagatt
    44281 gttgcacaca agcgagtagt caatggggcg tttgtaactt accccatcga tcttggaaat
    44341 gaagacatta ccaaactcca cgctcgcacc cgttaccgta cagggcggcg gggcatcaac
    44401 gagcacacgc aagaccatcg caaccgattt actccccgcc attacaggcg tgctcacaat
    44461 gctacacagc aataaccacg cccaggccga ataagctcct ttcatgctat tgctcctatc
    44521 cctggccttt gctgttttgc tcttcgtcca cctgacaggt atttcccgta cagcggaaga
    44581 acagcggcaa acgcgcacca taatcattca cataggtcag caccgggacg ctatccattt
    44641 tgacttttaa cggcacagag gtttttggcg atatcaccat gggggaaaat cccgctgccg
    44701 gattgccatt tttctgcgca ctggcgttac tgataaccac gaagtacggc gtcgggttat
    44761 tgacggtaaa ctcctgacca ctgagggtta aggtcacctt atgctgccac gggtttttca
    44821 tgctgacgtt ttccagcgcc tttggtcgcc agaacaactt aatgcgcgtc tgcagcgcta
    44881 tctgtagcgt gttaggtttg ttcgatttcg gcgggatctc acgcacgttg aaatagaaca
    44941 cgctctcgcg gtcggcaggc agcttgttga tatccggcat gccctgtacc ttcacctgac
    45001 cgttcatcat ggaatcgatg cgctgcactg gcggcaggac cgtcagtggc gagctaatcc
    45061 tgttaccctg tccatcttcc atccagcttt gtgccagata cggcattttc gggtcgttat
    45121 tgcgcagcgt gacgctgatt gatttatcac tttcgttaaa aatcaggcgt gtacggtccg
    45181 gcgtgacaga ggcctgtact gacaggctgg cggcagttat cagtaacgcc agcgtcgttg
    45241 cggccgttct ggcagagcaa aatagattcg tcatggatca ttccttaatc acgttattaa
    45301 ttggctgata ccgatactgg cgcttccgtt ggcatcacct gctgggtctg ctcctggatc
    45361 accggcttaa tttcatgagg catcgaagga gatatcgccc ctgtttgctg gcatggtaat
    45421 aacaggccat tgaacagatc ggctggcagc gggtcaggca gatgaatatc gcagtgggcc
    45481 acaccgcccc atgaaacgat catatgctcg ccaggtttta cgcccgcgag gtagacgttg
    45541 ccgtcatcgt caaccagacc gacgttctgc gcgctgtcgt ttttcacttc cgcgccaaac
    45601 ggcggataac tgccatcttg cagacgcagc accgccatcg ctttttgccc gctgatcacc
    45661 gagaacttac ggtagccaat ggccccttcg gtaagcgtgg cctgcaccac ggactgggtg
    45721 gcttcggcgt tttccggcag gttgtttagg tcgatatacg cctggttgcg gtagtagttg
    45781 ttgacgtctg ccaccactgc cttaccgaac atattggtgt aaaccgccgc gccatttcct
    45841 tccaccggaa caccagccac accgtcggca tcaatcagca gacgcgtacc gcccatattc
    45901 tgggtacggt ggagcgcgcc accttgtgcg gtgagcgtcg cgccgccctg taaggaaata
    45961 cccgccgagg tgtactgccc ttcatggtag ttagcgctga gatcgacctg cgccagcgat
    46021 ccgtcgtggc tgtaataacc gtcaacgctg gagtgattgt cgctggtgcc tacgttcaac
    46081 tggtaatggg ttgcgtcatc gacacggctg aaatacccca cctggctgct gtccgaaccg
    46141 ctgccgtagt tgccgttata gctgatggtg ctgctgtcac cccacggcat actgagcgat
    46201 atgtacacac ctttatcggc ctggttgtca tactcgtagc ggtatccggt catggaaatg
    46261 ctcatgttgc ggatactgcc caggttgaag tagtgcgaga gcatgacgtt gtagttggtc
    46321 tgttcgtcgc gatcccagta ggtatggcgg gtgtagttga gataaacaga cacaccggca
    46381 tccctgaagt tctggttata ggtggcggtg tacatctctt tgtcgttgcc ggtgcgcacc
    46441 atttcgctgt cgctggcatc gagatactcg ctcatggtca tgaagttctc ttccgagaag
    46501 cggtatccgg caaaagtgac gcggctgttc agttcatcga agtctttgga atagctcagg
    46561 cgaaacgagt tgccgtccag tgaacctttc ccgtaggcgg tctctttatc cagacgggta
    46621 tgcgagtggg tgatatcaaa ggccaccgca ccaaacacag acaggtcgcg accgacgcca
    46681 agcgccgccg actgatagtg ttcatctgcc agcgccccgc cgtatagcga ccagccgttg
    46741 gcaatccccc aggaagcttc gccgccggag aagaaaccgc cttccacgtg gtgcccccac
    46801 tcctgcgggc ggcccatcat cagtttatag cgcacctggc cggggcgagt caggaacggc
    46861 atcgaggcgg tgttgatgtc atattcctgc acctgaccgt tctgttcttc aatgcggata
    46921 tgcagcgtac cggagaccga atcgccaaga tcctggatgc ggaacggccc cgccgggacc
    46981 tgggtttcgt agatgacgcg gcccaactgg ctgacggtca ctttcgcggt ggtgtgcgcc
    47041 acgccggaga tatccggcgc atagccgcgc agattcggcg gcaacatttg atcgtcggtg
    47101 ctgatactgc cacccacgta gttaaagccg tcgaaaatat cagaattcag gtagtcttcg
    47161 ccaaggccaa gtttggcttt tagcgacggt aaggctcgcc aggcgtagta gcggctccac
    47221 tcccagtttt tttgcgtgtc atcaccgttg ataacatcgt catcattgct tttactatgc
    47281 aaataatcag tctgccagtc ggcacgaaga cgccatgcgc cgaggttcac cccaaccgtc
    47341 ccgttaccgc taatttcatt gctgtcatcc ccgccatttt cttcatgtcg tgtctgggcg
    47401 gtaatactgt agtcagcaat taaaccagat ataccgtcat cccagcgtga aggcggatcc
    47461 cagttgatgt cggtatattc aaggtaagcc tggggtaatg aaatgactaa cgctgactga
    47521 ctcaggtcag ctttaatatc aatgccttcc agttgaccgg gtttcaggca tttgccgtcg
    47581 tggatccatt gcaggttttt tgccacatcc tcttttaagc caaactgcgc gaccagttca
    47641 ggcgtcaggc aggcataggt tttactggca tcgttctcag aggcgtacca gtaaatatcg
    47701 tattcttccg tcagcggctg tttatttagt tgaacctgta aattgtattt cccaggttcg
    47761 acataaccct ggctggaaaa tcgcttcaga tcgatttttg tgtcgccttt taactccaga
    47821 aaacgggaat caaactgaat ttcattttca gcccaggcat tgacataact cccggacatt
    47881 gccaatgcaa cgcagcaggg aagtacccgc agtcggaaaa gagaatggtc aggcatacac
    47941 gtatccatcc gtaattatta taatttgcgc gaattccatt taaaaacccc atagcccgaa
    48001 agctatgggg gtatcactta gagataagta atctggaagg tcgtgttggc ttcaaaatta
    48061 cctaaatctg gcgcatcagc agcgcccacc agccaggctt taaagtccag cgtctgcttc
    48121 gctttgcctt tgttggtcgc cgtatcgttt acgattttct gttcgatacc cgcgccgctt
    48181 ttataagagg ttccctgagc gtcaccaatg gccaggctga cattgttaaa tgccgcacca
    48241 gtatcggtat tgtaaatggt gtagtaattg ccgctgttgg tagaagacgc gttaccggtg
    48301 aaggtagtgg tcatcgttgt ctgggtgtca aacacacagt cttgcagatg gatctggaaa
    48361 gctttcggtg tagaagtccc gttatttttc aggatccctg aaccaatgtc acccatatca
    48421 acttccagtt tgtgatcatc aggaacgata gagcacgggg aatcttcaat ggtgccgtag
    48481 aaacgtgctg taccattgtt gccagcggcg attgttgaag tagaagcgaa cgcacccatt
    48541 accattgttg cagcaatagc tgtttttaca aacttactca ttatttgtcc ttaaaccata
    48601 aataattaaa aatataaaca ttgcaataca ttgattcagt caatagcaat tatttatacc
    48661 cagccattga ataagctatc cgatctaact aacaagaaaa tagg
//
LOCUS       DBFIFI010000013        48212 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.013, whole genome shotgun sequence.
ACCESSION   DBFIFI010000013 DBFIFI010000000
VERSION     DBFIFI010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.013"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            24..548
                     /gene="lpfA1"
                     /locus_tag="N7B39_000776"
     CDS             24..548
                     /gene="lpfA1"
                     /locus_tag="N7B39_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA1"
                     /protein_id="HGU2420807.1"
                     /translation="MKKVVFALTALALTSGTVFAAESGDGTVKFTGEIVDSPCVLSVD
                     SQNQEVVLGQVQKSVFAAVGDKSPAKPFEIKLEDCDTTTMKKANVSFSGVGDADKSDL
                     ISVSTEAGAAKGVGIGIYDNSNTLVALNGGKASVDLSKGQTVLYFTANYVSTLATVTT
                     GYGNAQVDFNLSYE"
     gene            613..1299
                     /gene="lpfB"
                     /locus_tag="N7B39_000777"
     CDS             613..1299
                     /gene="lpfB"
                     /locus_tag="N7B39_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312457.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial biogenesis chaperone LpfB"
                     /protein_id="HGU2420808.1"
                     /translation="MKSKFVALALSLFLSQSVLAGGVGLSSTRVIYDGSKKEASLTVQ
                     NKSKTEEFLIQSWVDDAAGSKKTPFIITPPLFKLDPEKNNILRIVNITPGLPQDRESV
                     YWVNVKAIPSKSDDSENKNVLQIAVRTRIKLFYRPAGLKGDVKTAPNELRFTRNGNQL
                     RVDNPTVFNITFNQFFANDKEIEKAGMVPAKGALNITLPAGVGSVSKIKYNTINDFGS
                     SAEMITKNVD"
     gene            1328..3901
                     /gene="lpfC"
                     /locus_tag="N7B39_000778"
                     /pseudo
     CDS             1328..3901
                     /gene="lpfC"
                     /locus_tag="N7B39_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088424.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein LpfC"
     gene            3916..4971
                     /locus_tag="N7B39_000779"
     CDS             3916..4971
                     /locus_tag="N7B39_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2420809.1"
                     /translation="MKAAIALSLLGCVFGFSGKAFAGDAWGPCTPADGTTYHYNVDVD
                     VGIPDAAKNVAGTVLPDVLNWSNGQNVSLICECPDSYKNEKDTLVQGVSMLPPSGRTV
                     DSMKYYTLTEELEVATNIRISTSVYGFVPFKNQQALQTTGCNKVITTPYMGGAGLLSF
                     AITKPFIGDSVIPLTLIAELYASKTNKDYGTIPISSVSIQGRVTVTQDCEIKPGTVLD
                     VPFGEFPSSAFKNRQGQMPEGATEQEINLSFDCNNISDGIKVALRLEGATNADDPRAV
                     DMGNPDIGVLVKDSSGKILVPNDSSSTTLLNLSSLDSKTHRNAAIRLLALPISTTGKA
                     PKGGTFEGVTTIYLEME"
     gene            4976..5506
                     /gene="lpfE"
                     /locus_tag="N7B39_000780"
     CDS             4976..5506
                     /gene="lpfE"
                     /locus_tag="N7B39_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HGU2420810.1"
                     /translation="MKFKRLLHSGIASLSLVACGVNAATDLGPAGDIHFSITITTKAC
                     EMEKSDLEVDMGTMTLQKPAAVGTVLSKKDFTIELKECDGISKATVEMDSQSDSDDDS
                     MFALEAGGATGVALKIEDDKGTQQVPKGSSGTPIEWAIDGETTSLHYQASYVVVNTQA
                     TGGTANALVNFSITYE"
     gene            5761..7452
                     /gene="eptB"
                     /locus_tag="N7B39_000781"
     CDS             5761..7452
                     /gene="eptB"
                     /locus_tag="N7B39_000781"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312452.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HGU2420811.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GISAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVNDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPVNAQ
                     AFAQLKKAADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKEAAAN"
     gene            7544..7620
                     /locus_tag="N7B39_000782"
     tRNA            7544..7620
                     /locus_tag="N7B39_000782"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7578..7580,aa:Pro,seq:cgg)
     gene            8365..9972
                     /gene="dppA"
                     /locus_tag="N7B39_000783"
     CDS             8365..9972
                     /gene="dppA"
                     /locus_tag="N7B39_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HGU2420812.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            10123..11142
                     /gene="dppB"
                     /locus_tag="N7B39_000784"
     CDS             10123..11142
                     /gene="dppB"
                     /locus_tag="N7B39_000784"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HGU2420813.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            11152..12054
                     /gene="dppC"
                     /locus_tag="N7B39_000785"
     CDS             11152..12054
                     /gene="dppC"
                     /locus_tag="N7B39_000785"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HGU2420814.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            12065..13048
                     /gene="dppD"
                     /locus_tag="N7B39_000786"
     CDS             12065..13048
                     /gene="dppD"
                     /locus_tag="N7B39_000786"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HGU2420815.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            13045..14049
                     /gene="dppF"
                     /locus_tag="N7B39_000787"
     CDS             13045..14049
                     /gene="dppF"
                     /locus_tag="N7B39_000787"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HGU2420816.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKIGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(14079..15350)
                     /gene="yhjV"
                     /locus_tag="N7B39_000788"
     CDS             complement(14079..15350)
                     /gene="yhjV"
                     /locus_tag="N7B39_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HGU2420817.1"
                     /translation="MQHNTLPKHDQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFTMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKTWLILLYGILLCLSPLLKLIE"
     gene            15826..15933
                     /gene="ldrD"
                     /locus_tag="N7B39_000789"
     CDS             15826..15933
                     /gene="ldrD"
                     /locus_tag="N7B39_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HGU2420818.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(16020..17699)
                     /gene="bcsG"
                     /locus_tag="N7B39_000790"
     CDS             complement(16020..17699)
                     /gene="bcsG"
                     /locus_tag="N7B39_000790"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HGU2420819.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(17696..17887)
                     /gene="bcsF"
                     /locus_tag="N7B39_000791"
     CDS             complement(17696..17887)
                     /gene="bcsF"
                     /locus_tag="N7B39_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HGU2420820.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(17884..19455)
                     /gene="bcsE"
                     /locus_tag="N7B39_000792"
     CDS             complement(17884..19455)
                     /gene="bcsE"
                     /locus_tag="N7B39_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HGU2420821.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            19728..19916
                     /gene="bcsR"
                     /locus_tag="N7B39_000793"
     CDS             19728..19916
                     /gene="bcsR"
                     /locus_tag="N7B39_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HGU2420822.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDILALKQAFSLPGIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            19928..20680
                     /gene="bcsQ"
                     /locus_tag="N7B39_000794"
                     /pseudo
     CDS             19928..20680
                     /gene="bcsQ"
                     /locus_tag="N7B39_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
     gene            20677..23295
                     /gene="bcsA"
                     /locus_tag="N7B39_000795"
     CDS             20677..23295
                     /gene="bcsA"
                     /locus_tag="N7B39_000795"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HGU2420823.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGGALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTVGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            23306..25690
                     /gene="bcsB"
                     /locus_tag="N7B39_000796"
     CDS             23306..25690
                     /gene="bcsB"
                     /locus_tag="N7B39_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HGU2420824.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKTPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAVLAVLAVLAVLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            25697..26803
                     /gene="bcsZ"
                     /locus_tag="N7B39_000797"
     CDS             25697..26803
                     /gene="bcsZ"
                     /locus_tag="N7B39_000797"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HGU2420825.1"
                     /translation="MNVLRSGLVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            26785..30258
                     /gene="bcsC"
                     /locus_tag="N7B39_000798"
     CDS             26785..30258
                     /gene="bcsC"
                     /locus_tag="N7B39_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
                     /protein_id="HGU2420826.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADAPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVSALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWSSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWALQRRDYTAARA
                     AYQNVLTREPTNADAILGLTEVDIAAGDTAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPKQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLRDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            30340..32328
                     /gene="hmsP"
                     /locus_tag="N7B39_000799"
     CDS             30340..32328
                     /gene="hmsP"
                     /locus_tag="N7B39_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HGU2420827.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVHEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSISVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSVLQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            32511..33797
                     /gene="dctA"
                     /locus_tag="N7B39_000800"
     CDS             32511..33797
                     /gene="dctA"
                     /locus_tag="N7B39_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HGU2420828.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDALNNRAPDGKTHELSS"
     gene            34018..35514
                     /gene="yhjJ"
                     /locus_tag="N7B39_000801"
     CDS             34018..35514
                     /gene="yhjJ"
                     /locus_tag="N7B39_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HGU2420829.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(35610..36539)
                     /gene="kdgK"
                     /locus_tag="N7B39_000802"
     CDS             complement(35610..36539)
                     /gene="kdgK"
                     /locus_tag="N7B39_000802"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HGU2420830.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESDQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            36771..37538
                     /gene="pdeH"
                     /locus_tag="N7B39_000803"
     CDS             36771..37538
                     /gene="pdeH"
                     /locus_tag="N7B39_000803"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HGU2420831.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTAVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            37608..39668
                     /gene="yhjG"
                     /locus_tag="N7B39_000804"
     CDS             37608..39668
                     /gene="yhjG"
                     /locus_tag="N7B39_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HGU2420832.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGIAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(39850..41172)
                     /gene="yhjE"
                     /locus_tag="N7B39_000805"
     CDS             complement(39850..41172)
                     /gene="yhjE"
                     /locus_tag="N7B39_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2420833.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLSAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(41584..42597)
                     /gene="yhjD"
                     /locus_tag="N7B39_000806"
     CDS             complement(41584..42597)
                     /gene="yhjD"
                     /locus_tag="N7B39_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HGU2420834.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(42646..43545)
                     /gene="rcdB"
                     /locus_tag="N7B39_000807"
     CDS             complement(42646..43545)
                     /gene="rcdB"
                     /locus_tag="N7B39_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU2420835.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLNELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPHLSEFLYQHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQNPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEYLD"
     gene            44065..44667
                     /gene="yhjB"
                     /locus_tag="N7B39_000808"
     CDS             44065..44667
                     /gene="yhjB"
                     /locus_tag="N7B39_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGU2420836.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGIMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            complement(44718..46367)
                     /gene="treF"
                     /locus_tag="N7B39_000809"
     CDS             complement(44718..46367)
                     /gene="treF"
                     /locus_tag="N7B39_000809"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HGU2420837.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            46772..48169
                     /gene="ccp"
                     /locus_tag="N7B39_000810"
     CDS             46772..48169
                     /gene="ccp"
                     /locus_tag="N7B39_000810"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HGU2420838.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLDVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
BASE COUNT        11622 a        11981 c        13123 g        11486 t
ORIGIN      
        1 taatttttta aatggagttt ttcatgaaaa aggtcgtttt tgccttaacc gcactggcgt
       61 taacttctgg cactgtattc gcagcagagt ctggtgacgg cacggttaaa tttacaggcg
      121 agatcgtgga ttcaccttgc gtactgtccg ttgactctca gaaccaggaa gttgtgttgg
      181 gtcaggtaca aaaatctgta tttgctgcgg ttggtgacaa atccccggca aaacctttcg
      241 aaatcaaact ggaagattgc gataccacca cgatgaaaaa agccaatgtg agctttagtg
      301 gtgttggtga tgcagataaa tctgatctga tttctgttag cactgaagcg ggcgcggcca
      361 aaggtgttgg tatcggtatt tatgataaca gcaataccct ggtcgctctt aacggtggta
      421 aagcaagtgt tgatctgagt aaaggccaga cggttctcta ctttaccgct aactacgttt
      481 ctactctggc aacggtaacg accggttacg gcaatgcgca ggtcgatttc aacctgtctt
      541 acgagtaatc gtccgtttta caaatggcgg cgtaattgcc gccattagtc gtcaaagttc
      601 atggatcgaa tgatgaaaag taaatttgtt gcgttggctc tctctctgtt cctttcacag
      661 tcagttctgg cgggtggcgt cggacttagc tctactcgcg taatttatga tggcagtaag
      721 aaagaagcct cgctgaccgt acagaataaa agtaagacgg aagagtttct tatccaatcc
      781 tgggtggatg atgccgcggg cagcaaaaaa acaccgttta ttattacgcc cccactattc
      841 aagcttgatc cagaaaagaa caatattttg cgtattgtga atattacgcc cggcctgcct
      901 caggatcgcg aatcggtcta ctgggttaat gtgaaagcca ttccatctaa aagtgacgac
      961 agcgaaaaca aaaatgtttt gcagattgcc gttcgcaccc gaatcaaact tttttatcgc
     1021 ccggcaggtt taaagggtga tgtaaaaacc gcgccaaatg aactgcgatt tacgcgtaac
     1081 ggtaatcagc tccgcgttga taatccgaca gtttttaata ttacctttaa tcaatttttt
     1141 gcgaatgaca aagaaattga aaaagcaggc atggtacccg ctaaaggggc attaaatatc
     1201 acattacccg ccggggtagg tagcgtttct aaaattaaat ataacaccat caatgacttt
     1261 ggatcgtcag cggaaatgat cactaaaaat gttgattgat taatatcttt tcaggagaaa
     1321 tagctcaatg tcacggaaga ccgtttcacg tactttttct tccttttcta tctctgtggt
     1381 ggcggttgct gttgccagca cgttttctgc acatgccgga aagtttaacc ccaaattcct
     1441 ggaggacgtt cagggtgtgg gccagcatgt cgatttaacg atgttcgaaa aaggccagga
     1501 acagcagtta cccggcatct atcgtgtctc tgtttacgtc aatgaacaga gaatggaaac
     1561 ccgcacactg gaatttaaag aggccacaga agcacagcgc aaagcgatgg gggaatccct
     1621 tgtgccttgc cttagccgga cgcaactggc agagatgggc gtgcgcgttg aaagctttcc
     1681 ggccctcaat ctggtgtctg ccgaagcctg cgtaccgttt gacgaaatca ttcccctggc
     1741 ctcaagccat ttcgacttca gtgaacaaaa gctggtatta agtttccctc aggcggcgat
     1801 gcaccaggtg gcgcgcggca cggtgccgga atctttatgg gacgaaggta tccctgcgtt
     1861 gctgctggat tacagtttta gcgggagcaa cagtgaatat gactcgacgg ggtcttccag
     1921 cagttatgtc gatgataatg gcactgttca tcatgatgat ggtaaagata cgctaaagag
     1981 cgacagctac tacctcaact tacgcagcgg gctgaacctt ggcgcttggc ggttacggaa
     2041 ttacagtacc tggagccatt ccggtggcaa agcgcagtgg gataatatcg gtacgtcttt
     2101 gagtagggcc atcatccctt tcaaagcgca attaacaatg ggggatacgg cgaccgcggg
     2161 ggacattttt gacagcgttc agatgcgtgg cgcgatgtta gcctctgatg aagagatgct
     2221 gcccgacagc cagcgaggct ttgcacccat cgttcgaggg atcgccaaaa gcaacgccga
     2281 agtcagtatt gaacaaaacg gctacgttat ttatcgcacc tatgtgcagc ctggtgcctt
     2341 tgaaataaac gatctttacc ccactgcaaa cagcggtgat ctcacggtca ttattaaaga
     2401 agcggatggc agtgaacaac gctttattta gccattctca tcggtgccaa ttttccagcg
     2461 tgaaggccat ctcaaatata gctttgccgc aggtgaatat caggccggga attatgacag
     2521 cgcctcgccg cgtttcgggc agcttgatct gatctacggt ttaccgtggg ggatgacggc
     2581 ctacggcggc gtattaatct ctaataatta caatgcattt acattaggga tagggaaaaa
     2641 ctttggttat atcggggcga tttccattga tgtgacgcag gctaaaagcg aactgaataa
     2701 cgatcgcgat agccagggac aatcttatcg tttcttatat tccaagagct tcgaaagcgg
     2761 caccgatttc cgccttgcgg gctatcggta ctctaccagc ggtttctata ccttccagga
     2821 agccaccgat gtgcgcagtg acgctgacag cgactataac cgttatcaca agcgcagcga
     2881 aatacagggt aacctgacgc agcaattagg ggcctatggc tctgtttatt taaatttaac
     2941 gcagcaggat tactggaacg acgcaggtaa acagaacacg gtatcggcgg gttacaacgg
     3001 acgtattggc aaggtcagtt acagtattgc atatagctgg aataaaagcc ctgaatggga
     3061 tgaaagcgat cgcttgtggt ctttcaatat ttccgttcca ctaggccggg cctggagtaa
     3121 ctatcgcgtc acgaccgacc aggatggtcg taccaatcaa caggttgggg tcagcggaac
     3181 gctgcttgag gatcgcaacc tgagctacag tgtccaggaa ggctacgcca gcaacggtgt
     3241 gggtaacagc ggtaacgcta acgttggcta tcagggtggg tccggtaatg tcaacgtagg
     3301 ctatagctac gggaaagatt accggcagct caactacagc gttcgcggcg gcgtgatagt
     3361 tcatagcgaa ggcgtgacgc tttcccaacc gctaggcgaa accatgacgc tcatctccgt
     3421 acccggtgcg cgcaatgccc gcgtggtgaa taacggcggc gttcaggttg actggatggg
     3481 taacgcgatc gtgccttatg ccatgccgta tcgtgaaaac gaaatctcac tgcgtagcga
     3541 ttcgttgggt gacgatgttg acgttgaaaa tgcgttccag aaagtggtgc caacgcgtgg
     3601 agcgattgtc agagcgcgtt ttgatacccg cgttggttac cgcgtattaa tgacgctgct
     3661 tcgttccgcg ggcagcccgg tgccctttgg agcaacggca acgctaatca ccgataaaca
     3721 aaacgaggtg agcagtatcg ttggtgaaga aggacagctc tatattagcg gaatgccaga
     3781 ggaaggacgg gtattgatta aatggggtaa tgacgcgtcg cagcaatgcg tggcgcctta
     3841 taaattatcc ctggaattaa aacagggcgg aattattcct gtttcggcca attgccagta
     3901 agcaaggaga gcgaaatgaa agcggcaata gcattatcct tattaggatg cgtattcgga
     3961 ttttctggca aggcatttgc cggagatgcg tgggggccat gtacccccgc ggatggcacg
     4021 acttatcact ataatgtcga tgtggatgtt ggtatacctg atgcggcaaa aaacgtcgca
     4081 ggaacggttt taccggatgt ccttaactgg tctaatggac aaaacgtatc gctaatttgt
     4141 gaatgtcctg attcttataa gaatgaaaaa gatactctag tgcagggggt gagtatgctt
     4201 cccccttctg gccgtacggt tgacagtatg aaatattata ctttgacgga ggaactggag
     4261 gttgcgacga acattcgcat cagtaccagt gtgtatgggt ttgttccttt caaaaaccag
     4321 caggcgctgc aaacaaccgg atgcaacaaa gtcattacta cgccctatat gggcggcgca
     4381 ggtctacttt cctttgctat tactaaaccc tttatcggcg attccgtcat ccctctgacg
     4441 ttaattgccg aactgtatgc ctcgaaaaca aataaagatt acggaacaat acccatatct
     4501 tccgtatcta ttcaggggcg tgtcaccgta acccaggatt gcgaaattaa acccggcacg
     4561 gtgctggatg tgccatttgg tgaattcccc tcctcggcgt ttaaaaacag gcaagggcaa
     4621 atgcctgaag gtgcgacaga gcaagaaatt aacctcagct tcgattgcaa caatatctct
     4681 gatggtatca aggttgcgtt gcgccttgag ggggcaacca atgctgacga tccccgagcg
     4741 gtggatatgg gcaaccccga tattggcgta ctggtgaaag attccagcgg caaaattttg
     4801 gtaccgaatg attcaagcag caccacgtta ttaaatttga gcagcctgga ttctaaaact
     4861 caccgcaatg ccgcgattcg actgctggct ttaccgatta gcacgacggg taaagcgcct
     4921 aaaggcggga cgtttgaagg cgtaacgacc atatatcttg aaatggagtg atgccatgaa
     4981 gttcaaacga ttgctgcata gcggcatcgc cagtttgagt ctggttgcct gcggggtgaa
     5041 tgcggcgacg gatcttggcc cggcagggga tattcatttc tccatcacta tcaccactaa
     5101 agcttgcgag atggaaaaaa gcgatctcga agtcgatatg ggaacaatga cgctgcaaaa
     5161 acctgcggca gtcggtacgg tgttgagcaa gaaagatttc accattgaac tcaaagagtg
     5221 cgatgggata tccaaagcga ccgttgagat ggacagtcag tcggacagcg atgatgattc
     5281 catgtttgcc cttgaggctg gtggcgcaac gggtgttgcg ttgaagatag aggacgataa
     5341 aggaacgcag caagttccca aaggctccag cggaacgccg attgaatggg cgattgatgg
     5401 cgaaaccacg tcgcttcact accaggcgag ttatgtggtc gtcaacactc aggccactgg
     5461 tggcacagcg aatgcccttg tcaatttttc catcacctat gagtaagtta ctgggccagc
     5521 atcctggccc aacctatctc tgaatagtcg ttaatccgtt aaaatggtct attccggact
     5581 tgcacttttg taaatttgtg cttcatgcac actctttccc cacacttttt ccctttgctg
     5641 tggtctactt attcgcgcgt gtagatttta cttatctgac tacctccgca cttttcccct
     5701 gctgggcctg aaaagccgct aagcagggtg ttatcacctg tttgtccagg gtttgtttgc
     5761 atgagataca tcaaatcgat tacacagcag aagctgagct ttttgcttgc aatctatatt
     5821 ggccttttta tgaatggcgc ggttttttac cgccgcttcg gcagctatgc gcacgatttt
     5881 accgtctgga aaggcatttc tgctgttgtt gaactggccg ccaccgtact ggtgaccttc
     5941 tttttactac gtcttctttc gctgtttggc cgccgcagct ggcgtattct ggcatcgctg
     6001 gttgtgctct tttccgcagg tgccagctat tacatgacct tccttaatgt ggtcattggt
     6061 tatggcatca tcgcttccgt catgaccacc gatatcgacc tgtcaaaaga agttgttggt
     6121 ctgaacttta ttctctggtt aatcgccgtt agcgcattgc ctcttatcct tatctggaat
     6181 aaccgctgtc gctacacctt gctccgacaa ctgcgaaccc cagggcagcg tattcgcagc
     6241 ctggcggtcg tcgtactggc gggaattatg gtttgggcac cgattcgttt gctggatatc
     6301 cagcagaaga aagtggagag ggcgaccggc gttgatttgc cgagttatgg cggtgtcgta
     6361 gcgaactctt atctgccatc aaactggctt tctgcgttgg ggctgtacgc ctgggcgcgg
     6421 gtcgatgaat cttccgataa taattcattg cttaacccgg cgaagaaatt cacttatcag
     6481 gcaccgcaaa atgttaatga tacttatgtc gtgtttatca tcggtgaaac cacgcgttgg
     6541 gaccatatgg gcattttcgg ctatgagcgt aataccacgc cgaagctggc ccaggagaaa
     6601 aatctggcgg cgttccgtgg ttactcctgt gataccgcaa ccaaactctc tctgcgttgc
     6661 atgtttgtac gtcagggggg cgcggaagat aatccgcagc gcacattaaa agaacagaac
     6721 attttcgcgg tactgaagca gttaggattc agttctgacc tctacgctat gcagagcgaa
     6781 atgtggttct acagcaatac gatggcggac aatattgctt accgtgaaca gattggtgcg
     6841 gagccacgta accgtggcaa gccggtagat gatatgttgc tggtagacga aatgcagcaa
     6901 tcgctggggc gcaacccgga tggtaagcat ctaatcattc tgcataccaa aggttcgcac
     6961 tttaactaca cccagcgtta cccgcgcagc tttgcgcagt ggaagccgga atgtattggt
     7021 gttgatagcg gatgtaccaa agcgcagatg atcaactcct atgacaactc ggtgacctat
     7081 gtggatcact ttatctccag tgtgattgat caggttcgcg ataagaaagc gattgtgttc
     7141 tacgcagctg accacggcga gtcaattaat gaacgcgagc acctgcacgg cacgccgcgt
     7201 gaactggcac cgccggagca gttccgcgta ccgatgatgg tctggatgtc agataaatat
     7261 ctggaaaatc cggtcaatgc gcaggcgttt gcgcagctga aaaaagcagc cgacatgaaa
     7321 gtgccacgcc gtcacgtaga gctgtacgac accatcatgg gttgtcttgg ctatacttca
     7381 ccggatggtg gaattaacga aaacaacaac tggtgtcaca tcccgcagac aaaagaggca
     7441 gcggctaact aagaggcctg ctgactttct cgccgatcaa aaggcatttt gctattaagg
     7501 gattgacgag ggcgtatctg cgcagtaaga tgcgccccgc attcggtgat tggcgcagcc
     7561 tggtagcgca cttcgttcgg gacgaagggg tcggaggttc gaatcctcta tcaccgacca
     7621 aattcgaaaa gcctgctcaa cgagcaggct tttttgcatc tgcagcccat gaggaagaga
     7681 acctccgggg gcagggaggt tcgactcgac gtaggcctga taagacgcgt cagcgtcgca
     7741 tcaggcaacg gcaccgcact tattgtcgga tgcggcgtga acgccttatt cgacctacac
     7801 gccacacatt tccctgccaa atttgcgccc catatctcac tatccattaa cggatttgtg
     7861 acagaaacca ttgtcttttt ttatatttgc ttaaatcttt ttttgcgtta cttatggctg
     7921 gcgctggcat tttccgctgt gcgttttagc gttcatggta ttaatcgctc agatatccac
     7981 cttttcctca gatttttttc actaaatgca taaaacttaa tcaccaaatg ttgctaaata
     8041 ttaagctgat tgttctggtc atgttggagt agcacaaatt tctctgctgc aaatagctgt
     8101 ttgaagtttt tttaatcttt gtttgtggat tctcaccgtt ggtgtaaatc ccggttggct
     8161 gtattgacgt tttcacattc tgttgacaga ttgtaggtca cgaggggcat tttatggagg
     8221 atccgcactg ttacactgat gttaattagt acggcatccc cacctcataa cgttgacccg
     8281 accgggcaaa aaacaaaaaa ggtcaggcag cgacaaccca ctgcaaaggg ttaaaacaac
     8341 aaacatcaca attggagcag aataatgcgt atttccttga aaaagtcagg gatgctgaag
     8401 cttggtctca gcctggtggc tatgaccgtc gcagcaagtg ttcaggctaa aactctggtt
     8461 tattgctcag aaggatctcc ggaagggttt aacccgcagc tgtttacctc cggtaccacc
     8521 tatgacgcct cttccgtacc gctttataac cgcctagttg aatttaaaat cggcaccacc
     8581 gaagtgattc cgggcctcgc tgaaaagtgg gaagtcagcg aagacggtaa aacctatacc
     8641 ttccatctgc gtaaaggtgt gaagtggcac gacaataaag aattcaaacc gacgcgcgaa
     8701 atgaatgccg atgacgtggt gttctcgttc gatcgtcaga aaaacgcgca aaacccgtac
     8761 cataaagttt ctggcggcag ctacgaatac ttcgaaggca tgggcttgcc ggagctgatc
     8821 agcgaagtga aaaaggtgga cgacaacacc gttcagtttg tgctgactcg cccggaagcg
     8881 ccgttcctcg ctgacctggc aatggacttc gcctctattc tgtcaaaaga atatgctgat
     8941 gcgatgatga aagccggtac accggaaaaa ctggacctca acccaatcgg caccggtccg
     9001 ttccagttac agcagtatca aaaagattcc cgtatccgct acaaagcgtt tgatggctac
     9061 tggggcacca aaccgcagat cgatacgctg gttttctcta ttacccctga cgcttccgtg
     9121 cgttacgcta aattgcagaa aaatgaatgc caggtgatgc cgtacccgaa cccggcagat
     9181 atcgcccgca tgaagcagga taaatctatc aacctgatgg aaatgccggg gctgaacgtc
     9241 ggttatctct cgtataacgt gcagaaaaaa ccactcgatg acgtgaaagt tcgccaggct
     9301 ctgacctacg cggtgaacaa agacgcgatc atcaaagcgg tttatcaggg cgcgggtgta
     9361 tcagcgaaaa acctgatccc gccaaccatg tggggctata acgacgacgt tcaggactac
     9421 acctacgatc ctgaaaaagc gaaagccttg ctgaaagaag cgggtctgga aaaaggtttc
     9481 tccatcgacc tgtgggcgat gccggtacaa cgtccgtata acccgaacgc tcgccgcatg
     9541 gcggagatga ttcaggcaga ctgggcgaaa gtcggcgtgc aggccaaaat tgtcacctac
     9601 gaatggggtg agtacctcaa gcgtgcgaaa gatggcgagc accagacggt aatgatgggc
     9661 tggactggcg ataacgggga tccggataac ttcttcgcca ctctgttcag ctgcgccgcc
     9721 tctgaacaag gctccaacta ctcaaaatgg tgctacaaac cgtttgaaga tctgattcaa
     9781 ccggcgcgtg ctaccgacga ccataacaaa cgcgttgaac tgtacaagca agcgcaggtg
     9841 gtgatgcacg atcaggctcc ggcactgatc atcgctcact ccaccgtgtt tgaaccggta
     9901 cgcaaagaag ttaaaggcta tgtggttgat ccattaggca aacatcactt cgaaaacgtc
     9961 tctatcgaat aattaaaagc catacccggt ggtgctgtgc ctctgcgtgc agcgccatcc
    10021 ggcagcaata cttcattccc tgcccgacct gggcgggaat tgatttgtga gcaatacaga
    10081 cacgcagttc caggctgcgg gtcactacag agaatccggg ttatgttgca gtttattctc
    10141 cgacgtttgg gactcgtcat ccccacgttt atcggtatta cccttctcac atttgccttt
    10201 gtccacatga tcccgggcga tcctgtgatg atcatggcgg gcgaacgtgg gatctcccca
    10261 gagcgtcacg cgcagttgct ggctgaactc ggcttagata aaccgatgtg gcagcagtat
    10321 ctccattaca tttggggcgt tatgcatggc gatctaggca tttcaatgaa aagccgaatt
    10381 ccggtatggg aagagttcgt gccgcgcttt caggccacgc tggaacttgg cgtctgcgcg
    10441 atgatttttg cgaccgcggt cggcattccg gttggtgtgc tggccgcggt taaacgcggt
    10501 tccattttcg atcacacagc ggttggcctg gcgctgacag gttattcaat gcctatcttc
    10561 tggtggggca tgatgctgat catgctggtt tcggtgcact ggaacctgac acccgtctcc
    10621 ggtcgcgtga gcgatatggt gttcctcgat gattccaatc cgttaaccgg ttttatgctg
    10681 atcgacaccg ccatctgggg tgaagacggt aactttatcg atgccgtcgc ccatatgatc
    10741 ttgcccgcca ttgtgctggg tactattccg ctggcggtca ttgtgcgtat gacacgctcc
    10801 tcgatgctgg aagtgctggg tgaagattac atccgcaccg cgcgcgccaa agggttgacc
    10861 cgcatgcggg tgattatcgt ccatgcgctg cgtaacgcga tgctgccggt agtgaccgtt
    10921 atcggcctcc aggtgggaac attgctggcg ggggcgattc tgaccgaaac catcttctcg
    10981 tggcccggtc tggggcgctg gttgattgac gcactgcaac ggcgcgatta tccggtggtg
    11041 cagggcggcg tattgctggt ggcgacgatg attatcctcg tcaacctgct ggtcgatctg
    11101 ctgtatggcg tggtgaaccc gcgtattcgt cataagaagt aaggggtcat catgtcacag
    11161 gttactgaaa ataaagtgat tagcgcgccg gtaccgatga cccctttaca ggagttctgg
    11221 cactatttta aacgcaacaa aggcgcggtc gtcgggctgg tttacgtcgt catcgtgctg
    11281 tttatcgcga tctttgccaa ctggattgct ccctataacc cggcggaaca gttccgcgat
    11341 gcactgctcg ccccgccagc ctggcaggaa ggcggcagca tggcgcactt gctgggtacc
    11401 gatgatgtag gccgtgatgt gctgtcgcgg ctgatgtacg gcgcgcgctt gtcgctgctg
    11461 gttggctgtc tggtggtggt gttatcgctg attatgggcg ttattctcgg cctgatcgcc
    11521 ggttacttcg gcggcctggt cgataacatc attatgcgcg tggtcgatat catgctggcg
    11581 ctgccaagtc tgctgttggc actggtgctg gtggcgattt tcggcccgtc gattggtaac
    11641 gccgcgctgg cgctgacctt tgttgccttg ccgcactatg tgcgcttaac ccgcgccgcc
    11701 gtgctggtgg aggtgaatcg cgattacgtc accgcttctc gcgtggcagg agccggggcg
    11761 atgcgccaga tgtttattaa catcttcccg aactgccttg caccgctgat tgttcaggcg
    11821 tcgctcggtt tctctaacgc cattctcgat atggctgctc ttggtttcct cggcatgggg
    11881 gcgcagccgc caacgccgga gtggggcacc atgctctccg acgtgttgca gttcgcgcaa
    11941 agcgcctggt gggtcgtgac cttcccgggt ctggcgatcc tgctgacggt gctggcattt
    12001 aacctgatgg gtgacggtct gcgtgacgcg ctcgatccca aactgaagca gtaagaggtt
    12061 cgagatggcg ttattaaatg tagataaatt atcggtgcat ttcggcgacg aaagcgcacc
    12121 gttccgcgcc gtagaccgca ttagctacag cgtaaaacag ggcgaagtag tcgggattgt
    12181 gggtgagtct ggctccggta agtcggtcag ttcgctggcg attatggggc tgattgatta
    12241 tccgggccgc gtgatggcgg aaaagctgga gtttaacggt caggatttgc agcgtatctc
    12301 ggaaaaagag cgccgcaacc tggtgggtgc cgaagtggcg atgatcttcc aggacccgat
    12361 gaccagcctt aacccgtgct acaccgtggg tttccagatt atggaagcga ttaaggtgca
    12421 tcagggcggt aataagagca ctcgtcgtca gcgggcgatt gacttgttga atcaggtcgg
    12481 tattcccgat ccggcttcgc gtctggatgt ttacccgcat cagctttccg gcggcatgag
    12541 ccagcgcgtg atgattgcga tggccattgc ctgtcggcca aaactgctga ttgccgatga
    12601 accaaccact gcgctggacg tgaccattca ggcgcaaatc attgaactac tgctggagct
    12661 acagcagaaa gagaacatgg cgctggtgtt aattacccat gacctggcgc tggtggcgga
    12721 agcggcacat aaaatcatcg tgatgtatgc cggtcaggtg gtggaaacgg gcgatgcgca
    12781 cgccatcttc catgcgccgc gtcacccgta tactcaggca ttgctgcgtg cgctgccgga
    12841 atttgctcag gacaaagaac gtctggcatc gttgcctggc gttgttcccg gcaagtacga
    12901 ccgcccgaac ggctgcttgc ttaacccgcg ctgcccctat gccactgaca gatgtcgcgc
    12961 tgaagaaccg gcgctgaata tgctcgctga cgggcgtcag tccaaatgcc attacccact
    13021 tgatgatgcc gggaggccga cactatgagt acgcaagagg ccacctcgca acaaccgctg
    13081 ttgcaggcta tcgacctgaa aaaacattat ccggtgaaga aaggtatgtt cgctccggaa
    13141 cgtctggtga aggcgctgga cggcgtttcg tttaaccttg aacgtggcaa aacgctggcg
    13201 gtagtaggtg aatctggctg cggtaaatcg accctcggtc ggttgctgac gatgattgaa
    13261 acgcccaccg gcggcgagct gtattaccag gggcaggatc tgcttaagca cgatccgcag
    13321 gcgcagaagc tgcgtcggca gaaaatccag attgtcttcc agaaccctta cggttcgctg
    13381 aatccgcgta aaaaaatcgg gcaaattctt gaagagccgc tgctgatcaa caccagctta
    13441 agcaaagaac agcgtcggga aaaagccctg tcgatgatgg cgaaagtcgg cctgaaaacc
    13501 gagcactatg accgctatcc gcatatgttc tccggcggtc agcgtcagcg tatcgccatt
    13561 gcccgaggtc tgatgctcga cccggacgtg gtgattgccg atgaaccggt ctccgcgctg
    13621 gacgtctcgg tgcgtgcaca ggtgctcaac ctgatgatgg atttgcagca ggaactgggg
    13681 ctttcttatg tcttcatctc ccacgacctg tcggtggtgg agcacattgc tgatgaagtg
    13741 atggtgatgt acctgggccg ctgcgtggag aagggaacga aagaccaaat cttcaataac
    13801 ccgcgccatc cgtacactca ggcgctactt tccgcgacgc cgcgcctgaa tccggacgat
    13861 cgccgcgagc gcatcaaact caccggtgaa ctgccaagcc cgctgaatcc accgccgggc
    13921 tgcgcgttta acgcccgctg tcgtcggcgc ttcggcccct gcacccagtt gcagccgcag
    13981 ctaaaagact acggcggtca actggtagct tgttttgctg ttgatcagga tgaaaatccg
    14041 cagcgttaac tcacaaaacc ggggccatgc gctccggttt actcaatcag cttcaacagt
    14101 ggcgacaagc atagcaaaat gccgtaaagc agaatcagcc aggttttaaa tccgcgaagt
    14161 ttttccagtt gtgcgacttt atagatcagg aaaaacggaa tgaggcacga cacaatgcca
    14221 tataacgggc ttcccaactg aaagaacacc agcaccgata cacgaaacga aacccaaatc
    14281 gtcaacgtaa tgacgataaa agcgcagatc gccagagtca gcacgcgtga gttaattttc
    14341 ttggtatcaa taattcggct taacagattg agaataatgc ctttaatggc ctcgtggaaa
    14401 ccgagataaa tgccaaagaa tgcggtcagt acggcaaaga tattcagcac cgtagaggtg
    14461 atatgaatga tatgcccagg gatcacctgc gcggccagtg ccagtgccga gatattttgt
    14521 tcaaaggcag aaacggcttc ttcgtggcta attgagaagg taaacgaaaa ggcaaaaaac
    14581 agtatcaccg cgatgagcgt gatataacta attcggtggg tacgcagcgc gagccgggtt
    14641 gccagtactt tatccgcttc ccgtttacga taggcaatat tcattgggtt aagtacctga
    14701 ataaatactg cagaaaagaa gcaaaatgga atggtaagca ggacatcgcg aaaaaagacg
    14761 gaggcttgcg ggaaggcggt tatattggcg aaattccagt gcgggatcat cgtaaaaccg
    14821 aacacgacaa taatccctac tttgaccacc accattggcc cagaaatctt aaacagtaat
    14881 cgttcaccac cagacgcaat cgctaccagt acggcgaaaa tagcgacttt ataaagtaga
    14941 gattgtgaaa gatcggcatc ggttaaaccg aaggttttca ggtacgaggc gctgtcgaaa
    15001 accacggaga gagagtagat aaaaatcccg tggataatca tcaaaaagta gataaccccg
    15061 aggaaaattc cccagttctt ccccaggtaa tgactgataa tatcggtgta gtcattacag
    15121 gaatcgcttt cagaaagggt ttttaaataa atgtcctgca ctacccaggt ggcaggataa
    15181 gcaatgatcg ctgcggtaat aaatacccaa atgcccttca agccaatttg tactggcatc
    15241 agcacggttc cggcaccaat cgccatgcct atgcataata aaacccagcc gaagtcgtag
    15301 cgtgtaaacg gcaatttctg atcgtgtttc ggtagtgtgt tgtgctgcat aaagtcctgt
    15361 ccagaaagaa gtcggcctcg caaaaatgaa taaacccagt ttctcttgcg caacaggtgg
    15421 agaagtcgaa tacgcagtat gtaaaaaatg aggcaacaga ttcactgggg aggtcgcgag
    15481 gaggcgcaat tctacaaaat tgcgcatttc actaccagtc tggagtcagc ccggatatga
    15541 actgcaacta ttgcaaatta agaaattaag ctggaaggca tcacgcaatg aggaagattg
    15601 ccgagtgctg acagtaaaaa gcaatcgtaa aattagcggc aagccggaag ggttccggtg
    15661 aggcgcaatg ttgcgggggc ttaatccctg gtggcattgg ttgctggaga gagaaaaccc
    15721 ccgcacgttg caggtatgca cctgacaaca ccacgggggc taatcttgaa cctagaccac
    15781 tcaagaatag ccgcgaaacg ttgtcattac aacacaggcg gctatatgac gctcgcagag
    15841 ctgggcatgg ccttctggca tgatttagcg gctccggtca ttgctggcat tcttgccagt
    15901 atgatcgtga actggctgaa caagcggaag taacgtgtca tgcgggcgtc aggctgccgt
    15961 aatggcaatt tgcgcccgga ccagaccgca ggggggaaac tctgcggcct ttttcgtgct
    16021 tactgcgggt aaggcaccca gtcgccgccg ttcaggcgaa cgtacggttt atcctggtat
    16081 tgaataacta ctgcatttga gttctctgag accggtgctg tttgtggcaa cccactggtg
    16141 agttttttcc agtcaacatt gtcttcggtg aaaatcttgc catcaagaac gcgaaccacc
    16201 agatcggaga tagccaggaa gctgctcggt tggtcgatga caatcggtgc cccctgatgt
    16261 ggtgccttca tgccgaagaa tttcacccca acggggacgt cggtgataga cgggctaggg
    16321 atatcacgta ggccagatac ctgcattctg tcgcccttca gcgcgccgcc gtgttccggc
    16381 accacgacca ccatcacttt acgacccgat ttctccagtt cagtaaagaa ggcgtccagt
    16441 tcatcaaaga atttctgcgc ccgcgctttg taatccgctg ttttgctgac gcccggataa
    16501 tggttgccgt catgcagtgg aagcgtgttg tagaacgtgg cactacggct gtttttatct
    16561 ttttcggtaa cgtccagcca gcggttaagc acggcggtat cgtcataaac cggcgaacca
    16621 tcaaagccca gcaaaataac cggcagattt gtttgatcca tcaattcagt ctgcatgccg
    16681 ccattttcgc gaacttcttt caaaaaaccg ccgaactggc cgttatgtcc catcatcagg
    16741 tgctgggtaa agcccagttt cgacaggtta tcaaacagat agcagtcgtt atttgccggt
    16801 tgatacagat tagtgtgcga agtctgcccg cagctggcgc gcagtaaacg gatcgccgcc
    16861 gggccactgt aggaggtggc ggagttaaag ttcttgaact caatatcgaa atgcgaccac
    16921 agtggatgcg acatcaaccc agcggcttct atatccgacc aggaaagcga acagatgtta
    16981 atcaccagta gttcaaatgg ctgagcatca gcgggcagcg aagacgggaa ggtcgattta
    17041 cgtttcgcct ccgcgttata gaaattattc agccaggcgt taaggttcgc cgttgttggc
    17101 ggtgcagttt gtgcgggcat atcacccact accggtgcgc cacccgtcgc cgcaacggtt
    17161 gccgctgcgt taccacccgt cgttgttaca gtggtcgtcg gttgtccggc tggccacaag
    17221 gagaaacttg gtcccgccag ggtaagtacg ttcagccata gcagtatggc aaccacaaaa
    17281 acggtaatgc gaatccattg tgacaggaat aaccaggcca ctaataaaac aaaaatggcc
    17341 ccaatcatct gccagttaat aaagcgtgtg acaaggtcga ttaaataatc ggtactgaac
    17401 cccgccacct gcgaaccctg gctcattatg ctttccgggc caggcaacca ggtgtcatgc
    17461 cagaacaaag caaagccgat cggcagggca atccagtggc gcaagcgatg caggctgtag
    17521 cgcggaatgg gcatcagcag aaacgcggca aacaccaaat tgaggagcgg atggaagtta
    17581 agatatcccg cccacaacag gccgaactta accagaaaat agaagttcca gccggaaagg
    17641 ccgcgccagt attgccagag ggaagaaggc atggcggtat tttgcgtaaa ttgagtcatt
    17701 ttttggttgc cctggctttt tccgtgcggc gtaacgtccc agccggttta acataacgag
    17761 gtttagcaaa gaacaaacgt aaggtgtcgc gaatgcgtcg caaagagtgc cgcgccagat
    17821 agcccagcgg gaaaaatatc agtgcgcaaa cgacaataat ttcaatgata tcgctgatgg
    17881 tcatcatgat gagcgctcca cagcatcatc taacagtcgc atgggttctg gtattcgtcg
    17941 ccagtggcga ccatcgtgct cggcattgat gaccggttta gaactttgcg ttaaaggcag
    18001 cggcatgccc cattgttctg gggcaagcag gcgcatctgc accagctcgg cactgatttg
    18061 atcatcttca aaccagacca tacggtttga gaaaatgtcg ccagtaggca atgggaaaat
    18121 atgattcaac gcggtatcca gatcgttaat ccgacagaat gagagaaaca gcaccagccg
    18181 attaccgcca atggtcatga tatcgccggt acggttaggg cgacacagcg tcagggcttg
    18241 ttcaacgcgg atacccggta ccggacgtag ggcaaccaga acgcctttac cgtgggcagg
    18301 taatagaggg ttattcatca tgttgttgac ggcattacag aacacatccc acttctggaa
    18361 accacgcagt ttgagcggct gggtcattga cagcaaggta gtgatatctt ccggcacata
    18421 gcgactaaac ttctgccctt gcacgctttc gatcatcgtc agacaacggg agagtggcgc
    18481 attccacgga ataaccatat ttgcaccgca ggccaataac aaacgttcat cggtggcgcg
    18541 caggctagcg gtattttccc gcacgaggat tttcatcgca ctaccgcgct ggcgacgcag
    18601 ggtatgaatg ctgcgggcca gtggctcgat ttgcgcattt tgctgtaaag aaaagaccac
    18661 cgtcgccgcc tgagcggtac gggcttcatt gaacaggact tcgttattgt tgaacagttg
    18721 ccagtgttcc gatagcggcg gcgcaccttc cagtacagca acattactca gaatgcgttt
    18781 ttcgtcgctg cgtggttgga tctccgcctc ttcgctttga actaatgtcc agataccatt
    18841 ttgttgctga acgctaagct gctgacgggc gctgacccct ttttcgttgc accagaaggc
    18901 aatatccagc aaatgttggt cgccctgaaa acgcaaactg gcaagaccaa aaagtgaacg
    18961 gtattcctca agcaacaatg aaaattgttt atcgttatta tttccgggat taattaccaa
    19021 aagcgaacaa tggtttaacc tgctccattt attcatttta tccaaccatg agcgcagccg
    19081 ctcggcagga atgttttgcc atgcgttatt tgcgcaaaca agaataaaaa ggtaattatg
    19141 gggatcaata gaacactgca aatcacgggc caaatagtat agaccttttt catgatttag
    19201 cattgaaaat aattttattt tttccggccc ttgagaatca tctaattgaa agattttcgc
    19261 cggatcgctg tccatgctaa tgaccgcgac gtgtgcggtt tcagcctggg atgcaattgt
    19321 ttgattcgcc agactgatag catcttcatg gcgatcgacg ttaaaccacc agacgccgcc
    19381 tgctggcata tgtcgcagct catcccataa tgatgagata ccgatagaga atacagggtc
    19441 cacaatgtcc ctcatgtcac cattttgtct gattatcagt ttactagcga aagctaagaa
    19501 ataaacctac cattgaaatt aaaacttatc agatttagca tgtgaatcaa tttagcaggt
    19561 cacaagacgt gtgattttag tcttttcgtt tttaactttt tctgttgcgt aacggaaagt
    19621 caaaaagtga gcaaattccc gtctcgccgt aaacaataaa ccggcgaaat gctcacggtt
    19681 agaattgtct catgaacggt acggttattt catagggatc aagcaaaatg aataacaatg
    19741 aaccagatac tctgcctgat cccgcgatag gctatatctt ccagaatgat attttggcgt
    19801 taaagcaggc attttcactg cctggtattg attatgccga tatttcccaa cgcgaacagt
    19861 tggccgcggc attaaaacgc tggccgttgc tggcagagtt tgcgcaacaa aagtagggga
    19921 ttggtgaatg gccgtactgg gattgcaggg ggtgcgggga ggcgtgggga caacaaccat
    19981 caccgccgca ttagcctggt cattacaaat gttgggagaa aatgtcctgg tggtcgatgc
    20041 ctgcccggac aacttgttgc gcctgtcatt taatgttgat tttacccacc gtcagggctg
    20101 ggccagagct atgctggatg gctaggactg gcgcgacgct gggttgcgct acacctcgca
    20161 gctcgatttg ctgccttttg gtcagttatc cattgaagaa caagaaaatc cacagcactg
    20221 gcaaacccgg ctgagcgata tttgctccgg cttacagcaa ctaaaagcca gcgggcgtta
    20281 ccagtggatt ttaatcgact taccgcgtga tgcctcgcag ataacccacc agctgctgag
    20341 tttgtgcgat cactcgctgg caatcgtcaa tgtggatgcc aactgccata tccgactgca
    20401 tcagcaagcg ctgccggatg gcgcacatat tttgattaat aacttccgta ttggcagtca
    20461 ggttcaggac gatatttacc agctttggtt gcaaagccag cgccgattac tgccgatgct
    20521 cattcatcgt gatgaagcga tggctgaatg cctggcggct aagcaaccag taggtgaata
    20581 tcgcagtgat gcgctggcgg ctgaagagat attgacgctg gcgaactggt gcctgttgaa
    20641 ctattccggg ctgaaaacgc cagtcgggag tgcatcatga gtatcctgac ccggtggttg
    20701 cttatcccgc cggtcaacgc gcggcttatc gggcgttatc gcgattatcg tcgtcacggt
    20761 gcgtcggctt tcagcgcgac gctcggctgt ttctggatga tcctggcctg gatttttatt
    20821 ccactggagc acccgcgctg gcagcgtatt cgcgcagaac ataaaaacct gtatccgcat
    20881 atcaacgcct cgcgtccgcg tccgctggac ccggtccgtt atctcattca aacatgctgg
    20941 ttactgatcg gtgcatcgcg caaagaaacg ccgaaaccgc gcaggcgggc attttcaggt
    21001 ctgcagaata ttcgtggacg ttaccatcaa tggatgaacg agctgcctga gcgcgttagc
    21061 cataaaacac agcatcttga tgagaaaaaa gagctcggtc atttgagtgc cggggcgcgg
    21121 cggttgatcc tcggtatcat cgtcaccttc tcgctgattc tggcgttaat ctgcgttact
    21181 cagccgttta acccgctggc gcagtttatc ttcctgatgc tgctgtgggg gggagcgctg
    21241 atcgtacggc ggatgccggg gcgcttctca gcgctaatgt tgattgtgct gtcgctgact
    21301 gtttcttgcc gttatatctg gtggcgatat acctctacgc tgaactggga cgatccggtc
    21361 agcctggtgt gcgggcttat tctgctcttc gctgaaacgt acgcgtggat tgtgctggtg
    21421 ctcggctact tccaggtagt atggccgctg aatcgtcagc cggtgccatt gccgaaagat
    21481 atgtcgctgt ggccgtcggt ggatatcttt gtcccgactt acaacgaaga tctcaacgtg
    21541 gtgaaaaata ccatttacgc ctcgctgggt atcgactggc cgaaagacaa gctgaacatc
    21601 tggatcctcg atgatggcgg cagggaagag tttcgccagt ttgcgcaaaa cgtgggggtg
    21661 aagtatatcg cccgtaccac tcatgaacat gcgaaagcgg gcaacatcaa caatgcgctg
    21721 aaatatgcca aaggcgagtt cgtgtcgatt ttcgactgcg accacgtacc aacgcgatcg
    21781 ttcctgcaaa tgaccgtggg ctggttcctg aaagagaaac agctggcgat gatgcagacc
    21841 ccacaccatt tcttctcgcc ggacccgttt gaacgcaacc tggggcgttt tcgtaaaaca
    21901 ccgaacgaag gcacgctgtt ctatggtctg gtgcaggatg gcaacgatat gtgggacgcc
    21961 actttcttct gcggttcctg tgcggtgatt cgccgtaagc cgctggatga aattggcggc
    22021 attgctgtcg aaactgtgac tgaagatgcg catacttctc tgcgtttgca ccgtcgtggc
    22081 tatacctccg cgtatatgcg tattccgcag gcggcggggc tggcgaccga aagtctgtcg
    22141 gcgcatatcg gtcagcgtat tcgctgggcg cgcgggatgg tgcaaatctt ccgtctcgat
    22201 aacccgctca ccggtaaagg gctgaagttt gctcagcggc tgtgctacgt caacgccatg
    22261 ttccacttct tgtcgggcat tccacggctg atcttcctga ctgcgccgct ggcgttcctg
    22321 ctgcttcatg cctacatcat ctatgcgcca gcgttgatga tcgccctgtt cgtgctgccg
    22381 catatgatcc atgccagcct gaccaactcc aagatccagg gcaaatatcg ccactctttc
    22441 tggagtgaaa tctacgaaac ggtgctggcg tggtatatcg caccaccgac gctggtggcg
    22501 ctgattaacc cgcacaaagg caaatttaac gtcaccgcca aaggtggact ggtggaagaa
    22561 gagtacgtcg actgggtgat ctcgcggccc tacatcttcc ttgttctgct caacctggtg
    22621 ggcgttgcgg taggcatctg gcgctacttc tatggcccgc caaccgagat gctcaccgtg
    22681 gtcgtcagta tggtgtgggt attctacaac ctgattgttc ttggcggcgc agttgcggta
    22741 tcggtagaaa gcaaacaggt acgccgatcg caccgcgtgg agatgacgat gcccgcggca
    22801 attgcccgcg aagatggtca cctcttctcg tgtaccgttc aggatttctc cgacggtggt
    22861 ttggggatca agatcaacgg tcaggcgcag attctggaag ggcagaaagt gaatctgttg
    22921 cttaaacgcg gtcagcagga atacgtcttc ccgacccagg tggcgcgcgt gatgggtaat
    22981 gaagttgggc tgaaattaat gccgctcacc acccagcaac atatcgattt tgtgcagtgt
    23041 acgtttgccc gtgcggatac atgggcgctc tggcaggaca gctacccgga agataagccg
    23101 ctggaaagtc tgctggatat tctgaagctc ggcttccgtg gctaccgcca tctggcggag
    23161 tttgcgcctt cttcggtgaa gggcatattc cgtgtgctga cttctctggt ttcctgggtt
    23221 gtatcgttta ttccgcgccg cccggagcgg agcgaaacgg cacaaccatc ggatcaggct
    23281 ttggctcaac aatgatgata acgcgatgaa aagaaaacta ttctggattt gtgcagtggc
    23341 tatggggatg agtgcgttcc cctctttcat gacgcaggcg acgccagcaa cgcaaccact
    23401 gatcaatgct gagccagctg tagccgccca gacggaacaa aatccgcagg tggggcaagt
    23461 gatgccgggc gtgcagggcg ctgatgcgcc agtcgtggcg cagaacggtc cttcgcgtga
    23521 tgtgaagctg acctttgcgc aaattgcgcc gccgccgggc agcatggtgc tacgtggcat
    23581 taacccgaac ggcagcattg agtttggtat gcgcagcgat gaagtggtga cgaaggcgat
    23641 gctcaacctc gaatacacgc catcgccatc gttactgcct gtccagtcgc agttaaaggt
    23701 ttatctcaat gatgagctga tgggcgtgct gccagtgacc aaagaacagt tgggtaaaaa
    23761 aacgctggcg caaatgccca ttaatccact gtttattacc gacttcaacc gtgtgcggct
    23821 ggagtttgtc ggccattatc aggacgtgtg cgaaaacccg gccagcacca cgctttggct
    23881 ggatgttggg cggagcagtg gactggatct gacctatcag accctgaatg tgaagaatga
    23941 cctgtcacac ttcccggtgc cattctttga cccgcgtgat aaccgcacca acaccttgcc
    24001 gatggtcttt gcgggtgcgc cggatgttgg gctgcaacaa gcatctgcca ttgtcgcctc
    24061 gtggtttggt tcgcgttctg gctggcgtgg gcagaacttc ccggtgctct ataaccaact
    24121 gccggatcgt aatgccattg tctttgcaac caacgacaaa cggccggact tcctgcgcga
    24181 tcatccggcg gtaaaagccc cggtgattga gatgattaac catccgcaga atccttacgt
    24241 caaactgctg gtggtgtttg gtcgtgacga caaagacctg ttgcaggcag cgaaaggtat
    24301 cgctcagggt aacattctgt tccgtggtga aagcgtggta gtgaatgaag tgaaaccgct
    24361 gctaccgcgt aagccgtacg atgcgccgaa ctgggtgcgt accgatcgtc cggtcacctt
    24421 tggtgaactg aaaacctatg aagaacagtt acaatccagc ggtcttgagc cagcagcgat
    24481 taatgtttcg ctaaacctgc cgccagatct ctacctgatg cgcagtaccg gcattgatat
    24541 ggatattaat taccgctaca ccatgccgcc ggtgaaagac agttcgcgga tggatatcag
    24601 cctgaataac cagttcctgc aatccttcaa cctgagcagc aaacaggagg cgaaccgcct
    24661 gctgctgcgg attccggtat tacaaggttt gctggatggc aaaacggatg tctctattcc
    24721 ggcgctgaaa ctgggcgcga ccaaccagct gcgcttcgac tttgagtata tgaacccgat
    24781 gcctggcggt tcggtggata actgtattac cttccagccg gtgcagaatc atgtggtgat
    24841 tggtgacgac tccaccatcg acttctcgaa gtattaccac ttcatcccga tgccggatct
    24901 acgcgccttt gctaacgcgg gcttcccgtt cagccggatg gcggatctgt cgcaaaccat
    24961 caccgtgatg ccgaaaacgc ctaacgaagc acagatggaa acgttgctga atactgttgg
    25021 ttttatcggc gcacagacgg gcttcccggc gattaatctg acggtgaccg atgatggcag
    25081 caccattcag ggcaaagatg ccgacatcat gatcatcggt ggtatcccgg acaaactgaa
    25141 agacgataag cagatcgacc tattggtgca ggcgaccgaa agctgggtga aaacaccgat
    25201 gcgccagacc ccgttccccg gcattgtgcc ggacgagagc gatcgcgcgg cagaaaccca
    25261 gtcaacgctg acctcttccg gtgcgatggc ggcggtgatt ggcttccagt cgccgtataa
    25321 cgaccagcgc agcgtgattg cgctgctggc agatagccca cgcggttatg aaatgcttaa
    25381 cgatgcggtg aacgatagcg gcaaacgcgc caccatgttc ggttcggtcg cggtgatccg
    25441 cgagtccggt atcaacagcc tacgtgttgg cgacgtttat tacgtaggtc atctgccgtg
    25501 gttcgagcgc ttgtggtatg cgctggcaaa ccatccgatt ctgctggcgg tgctggcggt
    25561 gctggcggtg ctggcggtgc tggcggtgct ggcggtgctg gcggctatca gtgtgatatt
    25621 gctggcatgg gtactgtggc gtttgctgcg tattatcagc cgtcgtcgtc ttaacccgga
    25681 taacgagtaa ttgaagatga atgtgttgcg tagtggactc gtgacgatgc tgctgttggc
    25741 tgcctttagt gttcaggcag cctgtacctg gcctgcctgg gagcagttta aaaaggatta
    25801 catcagtcag gaagggcgcg tcatcgaccc cagcgacgcg cgcaaaatca ccacctctga
    25861 agggcaaagt tacggcatgt tctttgccct ggcggctaac gaccgtgcag ctttcgataa
    25921 tattctcgac tggacgcaga acaatctcgc tcagggttct ttaaaagaac gtttgcccgc
    25981 ctggctgtgg ggcaagaaag agaacagtaa gtgggaagtg ctggacagca attcggcctc
    26041 cgatggtgat gtctggatgg cttggtcgtt gctggaggcg gggcgtttgt ggaaagagca
    26101 gcgttatacc gacatcggca gcgcgttgct aaaacgtatt gcgcgggagg aagtggtgac
    26161 ggtgcctggg ctgggttcca tgttgttacc gggcaaagtg ggttttgctg aggataacag
    26221 ctggcgtttt aaccccagct acctgccgcc gacgctggcg cagtatttca cccgctttgg
    26281 cgcgccgtgg actacgctgc gcgaaaccaa tcaacgttta ttgctggaaa ccgccccgaa
    26341 aggcttttcg ccagactggg tgcgctatga gaaagacaaa ggctggcagc taaaagccga
    26401 aaaaacattg atcagcagct acgacgctat ccgcgtttac atgtgggtag gcatgatgcc
    26461 tgacagcgat ccgcagaaag cgcggatgct caaccggttt aaaccgatgg cgacattcac
    26521 tgagaaaaac ggttatccgc cggaaaaagt ggatgtggct acggggaaag cgcagggtaa
    26581 aggaccggtc ggtttttctg ccgccatgct gcccttttta caaaaccgtg atgcgcaggc
    26641 cgttcagcgc cagcgcgtgg ccgataactt tcccggcagc gatgcctatt acaactatgt
    26701 gctgaccctg tttggacaag gctgggatca acaccgtttc cgcttctcga caaaaggtga
    26761 gttattacct gactggggcc aggaatgcgc aaattcacac taaacatatt cacgctttcc
    26821 ctcggtctgg ccgtcatgcc gatggtcgag gcagcaccaa ccgctcagca acagttgctg
    26881 gagcaagttc ggttaggcga agcgacccat cgtgaagacc tggtgcagca gtcgttgtat
    26941 cggctggaac ttattgatcc gaataacccg gacgtcgttg ccgcccgttt ccgttctttg
    27001 ttacgtcagg gcgatattga tggcgcgcaa aaacagctcg atcggctgtc gcagttagcg
    27061 ccgagttcaa atgcgtataa atcgtcgcgg actacgatgc tactttccac gccggatggt
    27121 cgtcaggcac tgcaacaggc aagattgcag gcgactaccg gtcatgcgga agaagctgtg
    27181 gcgagttaca acaaactgtt caacggtgcg ccgccggaag gtgacattgc tgtcgagtac
    27241 tggagtacgg tggcgaaaat tccggctcgc cgtggcgaag cgattaatca gttaaaacgc
    27301 atcaatgcgg atgcaccggg caatacgggc ctgcaaaaca atctggcgct attgctgttt
    27361 agtagcgatc gccgtgacga aggttttgcc gtcctggaac agatggcgaa atcgaacgcc
    27421 gggcgcgaag gggcctctaa aatctggtac gggcagatta aagacatgcc cgtcagtgat
    27481 gccagtgtgt cggcgctgaa aaaatatctt tctatcttta gtgatggcga tagcgtggcg
    27541 gctgcgcaat cgcaactggc agaacagcaa aaacagctgg ccgatcctgc tttccgcgct
    27601 cgtgcgcaag gtttagcggc ggtggactct ggtatggcgg gtaaagccat tcccgaacta
    27661 caacaggcag tgcgggcaaa cccgaaagac agtgaagctc tgggggcgct gggccaggca
    27721 tattcacaga aaggcgatcg cgccaatgca gtggcgaatc tggaaaaagc cctcgcactg
    27781 gatccgcaca gcagcaacaa cgacaaatgg aacagtctgc tgaaagtaaa ccgctactgg
    27841 ctggcgatcc agcagggcga tgctgcgctg aaagccaata atcctgaccg ggcagaacgc
    27901 ctgttccagc aggcgcgtaa tgtcgataac accgacagtt atgcagtgct ggggctgggc
    27961 gatgtggcga tggcgcgaaa agattatccc gccgccgaac gttattatca gcagaccttg
    28021 cgtatggaca gcggcaacac taacgccgtg cgcgggctgg caaatattta ccgccagcaa
    28081 tcgccagaaa aagctgaagc gtttatcgcc tcactctctg ccagtcagcg gcgtagcatt
    28141 gatgatatcg aacgcagcct gcaaaacgac cgtctggcac agcaggcaga ggcactggaa
    28201 aaccagggca aatgggcgca ggcggcagca cttcagcggc aacgactggc gctggacccc
    28261 ggcagcgtat ggattactta ccgactttcg caggatctct ggcaggccgg acaacgcagc
    28321 caggccgata cgttaatgcg caatctggcg cagcagaagc cgaacgaccc ggagcaggtt
    28381 tacgcttacg ggctgtacct ctctggtcat gaccaggaca gagcggcgct ggcgcatatc
    28441 aacagcctgc cgcgtgcgca gtggagcagc aatattcagg agctggttaa tcgactgcaa
    28501 agcgatcagg tgctggaaac cgctaatcgc ctgcgagaaa gcggcaaaga agctgaagcg
    28561 gaagcgatgc tgcgtcagca accaccttcc acgcgtattg acctcacgct ggctgactgg
    28621 gcgctgcagc gacgtgatta caccgccgcc cgcgctgcat atcagaatgt cctgacgcgg
    28681 gagccaacta acgccgatgc cattcttggt ctgacggaag tggatattgc tgccggtgac
    28741 acagcggcgg cacgtagcca gctggcgaaa ctgcccgcta ctgataacgc ctcgctgaac
    28801 acacagcggc gcgtggcgct ggcgcaggcg cagcttggag ataccgcagc ggcgcagcag
    28861 acgtttaata agttgatccc gcaggcaaaa tctcagccac cgtcgatgga aagcgcgatg
    28921 gtgctgcgcg acggtgcgaa gtttgaagcg caggcgggcg atccaaagca ggcgctggaa
    28981 acctacaaag acgccatggt cgcatccggt gtgactacga cgcgtccgca ggataacgac
    29041 acctttaccc gactgacccg taacgacgag aaagatgact ggctgaaacg cggcgtgcgc
    29101 agcgatgcgg cggacctcta tcgccagcag gatcttaacg tcaccctcga gcatgattat
    29161 tggggttcga gcggcaccgg tggttactcc gatctgaaag cgcacactac catgttgcag
    29221 gtggatgcgc cgtattctga cgggcggatg ttctttcgca gtgatttcgt caatatgaac
    29281 gtcggcagtt tctccactaa tgccgatggc aaatgggatg acaactgggg cacctgtaca
    29341 ttacgggact gtagcggcaa ccgcagccag tcggattccg gtgccagcgt ggcggtcggc
    29401 tggcgaaatg acgtctggag ctgggatatc ggcaccacgc cgatgggctt caacgtggtg
    29461 gatgtggtcg gcggcatcag ttacagcgat gatatcgggc cgctgggtta caccgttaac
    29521 gcccaccgtc gtcccatctc cagttctttg ctggcctttg gtgggcaaaa agactccccg
    29581 agcaataccg ggaaaaaatg gggcggcgtg cgtgccgacg gcgtggggct aagcctgagc
    29641 tacgataaag gtgaagcaaa cggcgtctgg gcatcgctta gtggcgacca gttaaccggt
    29701 aaaaatgtcg aagataactg gcgtgtgcgc tggatgacgg gctattacta taaggtcatc
    29761 aaccagaaca atcgccgcgt cacaatcggc ctgaacaaca tgatctggca ttacgacaaa
    29821 gacctgagtg gctactcact cggtcagggc ggttactaca gcccgcagga atacctgtcg
    29881 tttgccatac cggtgatgtg gcgggagcgc acggaaaact ggtcgtggga gctgggggcg
    29941 tctggctcgt ggtcgcattc acgcaccaaa accatgccgc gttatccgct gatgaatctg
    30001 atcccgaccg actggcagga agaagctgcg cggcaatcca acgatggcgg cagcagtcag
    30061 ggcttcggct atacggcgcg ggcattactt gaacgacgtg ttacttccaa ctggtttgtc
    30121 ggcacggcaa ttgatatcca gcaggcgaaa gattacgccc ccagccattt cctgctctac
    30181 gtacgttatt ccgccgccgg atggcagggt gacatggatt taccgccgca gccgctgata
    30241 ccttacgccg actggtaagt tttcagatag cgcctctctt aatgccgctg cgatcgggta
    30301 tactcgggcg gcaatctggg atttccgggg ggagacaatt tgcgcgtaag tcgctcgtta
    30361 acaatcaagc agatggcaat ggtggcagcc gttgtcctgg tgttcgtttt tattttttgc
    30421 accgttttgc tgttccatct ggtccagcag aatcgctata acacggctac gcaactggaa
    30481 agcattgctc gctctgtccg cgaaccttta tcttcagcca ttttgaaagg tgatattccc
    30541 gaagcggaag ctattcttgc cagcattaaa ccggcaggcg tggtcagccg tgccgatgta
    30601 gtgctgccta accagttcca ggcgctgcgt aaaagtttta ttccagagcg tccggtgccg
    30661 gtaatggtta ctcgcctgtt tgagctaccg gttcaaatct cgctgggcgt ctactcgctc
    30721 gaacgtccgg caaatccgca gccaattgcc tatctggtgc tacaggcgga ttccttccgt
    30781 atgtataagt tcgtgatgag caccctctca acgttagtga ccatttactt acttttgtcg
    30841 ttaatattga cggtggcgat tagctggtgc attaaccgcc tgattttgca tccgttacgc
    30901 aatattgctc gcgaacttaa cgccatccca gcccaggagc ttgttggtca ccaactggca
    30961 ttaccgcgtc tgcatcagga cgatgaaatc ggtatgttgg tgcgcagtta caacctcaac
    31021 cagcaattgc tgcagcgcca ttatgaagaa cagaacgaaa atgcgatgcg cttcccggtg
    31081 tcggatttgc cgaacaaagc cttgctgatg gagatgctgg agcaggttgt cgcgcgtaaa
    31141 caaaccaccg cgctgatgat catcacctgt gaaactctgc gtgatactgc gggcgtgctg
    31201 aaagaggcgc aacgagaaat tctgctgctg acgctggtgg aaaaactcaa atcggtactg
    31261 tcgccacgta tgatcctcgc gcagattagc ggttatgact ttgctgtcat tgccaacggt
    31321 gtacatgaac cgtggcacgc aatcactttg ggtcagcaag tgctcactat catgagcgag
    31381 cgcctgccga ttgaacgtat tcaactccgt ccgcactgta gcattagcgt ggcgatgttc
    31441 tacggcgatc tcaccgccga acagctttac agtcgcgcta tttctgcggc atttaccgct
    31501 cgccataaag gcaagaatca gattcagttc tttgatccgc agcagatgga agccgcccag
    31561 aagcggttga cggaagagag cgatatcctt aatgcactgg aaaatcatca gtttgctatt
    31621 tggttacagc cacaggtcga gatgaccagc ggtaaactgg tcagtgcgga agtgttactg
    31681 cgtatccagc aaccggatgg cagttgggac ctgccggatg gcttaatcga tcgcattgag
    31741 tgctgtgggc tgatggttac cgtcggtcac tgggtgctgg aagagtcctg tcgattgctt
    31801 gcagcctggc aagagcgcgg cattatgctg cccttgtcgg taaacctctc tgtgctgcaa
    31861 ctgatgcacc cgaatatggt ggcggatatg ctggaactgt taacccgcta tcgcattcag
    31921 ccgggaacac tgattctgga agtgacagaa agccgacgta ttgacgaccc tcatgctgcg
    31981 gtggcaatcc tccgtccgct gcgtaatgcc ggagttcggg tggcgctgga tgatttcggc
    32041 atgggctacg cagggctgcg tcagctgcag catatgaaat cgttgccaat cgacgttctg
    32101 aaaatcgaca aaatgtttgt tgaaggcttg ccggaagata gcagcatgat tgctgcaatt
    32161 atcatgctgg cgcagagcct gaacttacaa atgattgccg aaggcgtgga gactgaagca
    32221 caacgcgact ggctggcaaa agcgggcgtt ggtattgccc agggcttcct ttttgctcgt
    32281 ccactcccta ttgaaatctt cgaagagagt tacctggaag aaaagtagct accccaaact
    32341 gattacaaaa ctttaaaaag tgctggtttg tgcgagccag ctcaaacttt ttaacctttt
    32401 tgtttcaatt atgatccagg tacatttctg tgatgttgtc tgggtgttat tttaaggccg
    32461 caggtacccc ataaccttac aagacctgtg gttttactaa aggacaccct atgaaaacct
    32521 ctctgtttaa aagcctttac tttcaggtcc tgacagcgat agccattggt attctccttg
    32581 gccatttcta tcctgaaata ggcgagcaaa tgaaaccgct tggcgacggc ttcgttaagc
    32641 tcattaagat gatcatcgct cctgtcatct tttgtaccgt cgtaacgggc attgcgggca
    32701 tggaaagcat gaaggcggtc ggtcgtaccg gcgcagtcgc actgctttac tttgaaattg
    32761 tcagtaccat cgcgctgatt attggtctta tcatcgttaa cgtcgtgcag cctggtgccg
    32821 gaatgaacgt cgatccggca acgcttgatg cgaaagcggt agcggtttac gccgatcagg
    32881 cgaaagacca gggcattgtc gccttcatta tggatgtcat cccggcgagc gtcattggcg
    32941 catttgccag cggtaacatt ctgcaggtgc tgctgtttgc cgtactgttt ggttttgcgc
    33001 tccaccgtct gggcagcaaa ggccaactga tttttaacgt catcgaaagt ttctcgcagg
    33061 tcatcttcgg catcatcaat atgatcatgc gtctggcacc tattggtgcg ttcggggcaa
    33121 tggcgtttac catcggtaaa tacggcgtcg gcacactggt gcaactgggg cagctgatta
    33181 tctgtttcta cattacctgt atcctgtttg tggtgctggt attgggttca atcgctaaag
    33241 cgactggttt cagtatcttc aaatttatcc gctacatccg tgaagaactg ctgattgtac
    33301 tggggacttc atcttccgag tcggcgctgc cgcgtatgct cgacaagatg gagaaactcg
    33361 gctgccgtaa atcggtggtg gggctggtca tcccgacagg ctactcgttt aaccttgatg
    33421 gcacatcgat atacctgaca atggcggcgg tgtttatcgc ccaggccact aacagtcaga
    33481 tggatatcgt ccaccaaatc acgctgttaa tcgtgttgct gctttcttct aaaggggcgg
    33541 caggggtaac gggtagtggc tttatcgtgc tggcggcgac gctctctgcg gtgggccatt
    33601 tgccggtagc gggtctggcg ctgatcctcg gtatcgaccg ctttatgtca gaagctcgtg
    33661 cgctgactaa cctggtcggt aacggcgtag cgaccattgt cgttgctaag tgggtgaaag
    33721 aactggacca caaaaaactg gacgatgcgc tgaataatcg tgcgccggat ggcaaaacgc
    33781 acgaattatc ctcttaatct catcacttat gcccgcactc ccttccccga gtgcgggtgt
    33841 ttgcgcataa ttgacccctg tgcctgaact taccacctgg gtgagttcag gtttatttca
    33901 cttcttccgt gacattttca tgttcttgcg gtctaacacg aagtgttttt aacgtcattt
    33961 agactgaccg acaacgggca gtttttttat taccaggata gttgatcagg ggttcacatg
    34021 cagggcacaa aaattcgact tttagcgggc ggtttgctga tgatggccac tgctggctat
    34081 gtgcaggcag atgcgctcca gcctgatcca gcatggcaac aggggacgct ttccaacggt
    34141 ttacagtggc aagtgctgac caccccccag cgtcccagcg atcgtgttga aattcgcctg
    34201 ctggttaata ccggttcgct cgccgaaagt acacaacaga gcggttacag tcacgccatc
    34261 cctcgtattg cgctaacgca aagcggtggc cttgacgcag cacaggcgcg ttcattgtgg
    34321 cagcagggga tcgaccctaa acgcccgatg ccgccggtaa ttgtctctta tgacaccacg
    34381 ctgtttaatc tgagtttgcc caataaccgt aacgatttgc tgaaagaggc gctctcttat
    34441 ctggcaaatg ccactggcaa actgactatc acgccagaaa ccatcaacca cgcgctgcaa
    34501 agtcaggaca tggtggcaac ctggcctgcc gatactaaag agggctggtg gcgctatcgt
    34561 ctgaaagggt caaccttgtt aggtcacgat cctgccgatc cgctgaaaca acccgttgaa
    34621 gcggaaaaaa ttaaagattt ctatcagaaa tggtacaccc cggatgcaat gacgctgctg
    34681 gtggtgggaa acgtggatgc gcgctcggtc gtcgaccaaa tcaacaaaac gtttggcgaa
    34741 ctgaaaggca aacgtgaaac accggctccg gtgccgacgc tttctccgct gcgtgcggaa
    34801 gcggtgagta ttatgactga cgcggtgcgt caggaccggt tatctatcat gtgggatacg
    34861 ccgtggcagc cgattcgtga atcagccgca ctgctgcgct actggcgtgc ggacctggcc
    34921 cgtgaggcgc tgttctggca tgttcagcaa gcgttaagcg ccagtaacag caaagacatc
    34981 ggtcttggat ttgactgccg tgtgctgtat ctgcgtgcgc agtgtgccat caacatcgaa
    35041 tcaccaaacg acaagctgaa cagcaacctt aatctggtgg cgcgtgaact ggcgaaggtt
    35101 cgcgataaag gtctgccgga agaagagttc aatgcgttag tggcgcaaaa gaaactggag
    35161 ctgcagaaac tgtttgccgc ctatgcacgg gctgataccg atattctgat gggccagcgg
    35221 atgcgttcgt tgcaaaatca ggtcgtcgat atcgcgccgg agcagtatca gaaactgcgg
    35281 caggatttcc ttaacagcct gactgtggag atgttaaatc aggatctgcg tcagcagttg
    35341 tcgaatgata tggcgttaat actgctgcag ccgaaaggcg agccggaatt taacatgaaa
    35401 gcgttgcagg cggcctggga tcaaatcatg gccccatcta ccgccgctgc gaccacctct
    35461 gtcgccacgg atgacgtaca tcctgaagtg acggatattc cacctgcaca gtaatgcctg
    35521 atggtgatga gttagcacaa ccatcaggta tttatggatg agctggatag aggggtaact
    35581 ctttatccag ccttttgcat atgctgcgtt tacgctggca tcgcctcacg cgggataatc
    35641 gcgccgcgat actgaataac ggtacttgcg gtcaggtgcc cgcgtttcgc tgcgttttcc
    35701 gcactgccgc ccgtcaaacg taccgccaga taaccggcac tgaaagagtc gccagctgcg
    35761 gtggtatcga tcactttttc tttcggcagt ttcaccgccg gaacatccac taacccttcg
    35821 ccagcaatgg acaccaggca agaatccgcc ccgcgtttca ccaccacttc tttcacgccc
    35881 gcgttatggg tgcgcgcaat gacgtcttcc accggctgtt gaccccacag cgcgtcttca
    35941 tcgtccagcg tcaggaaggc gatatccgtg cattccagca tttgttggta cacctgctgt
    36001 gtctcttctt tgctggccca caggcgcgga cgatagttat tgtcgaaaat cacttttccg
    36061 ccgttggcgc ggcattcgcg cagcagggaa agcagctttt cgcggctggt cgggcttaag
    36121 atcgccaggc taatcccgct caggtagaga taatcgaaat tcgccagctc ttcgcaaatc
    36181 gccgcggatt gatcgctctc cagccagaat ttggcggcgg cttcgttacg ccagtagtag
    36241 aacgtacgtt cgccggtgct gtcggtttca atgtagtaaa ggcccggcag acggttttcc
    36301 atccgttggg tcagggaagt atcaacgttc tcgccgtgcc aggcgtccag catctgctgg
    36361 ctaaaactgt ccgttcccag cgccgttacg taatgaacgg ttaatgccgc aggatcgacc
    36421 tgacgggcga tatagacgga agtgttcagg gtatcgccgc cgaaaccgcg cttaacgtcc
    36481 gcgcctttct cggaaagctc aatcatgcat tcgccaatca cggcaatctt tttggacata
    36541 gtcgtgaacc tgatctgtaa aaaattagcg ttagtttgcg ctgtggtggt ttgctggtca
    36601 accatattaa aacagtgttc cattattttt ttgagccaga acgtattcct gaaagattcc
    36661 gttgtggagt ggggaaattt accccggcca ggcggtaaag ttctgccctt gcgcgccgat
    36721 aatctttgtc gagtccgggc tgcatcactt ttaaacacag gacatctttg atgataaggc
    36781 aggttatcca gcgaataagc aaccctgaag caagcatcga gagcttgcag gaacggcgtt
    36841 tttggttgca gtgtgagcgt gcttacacct ggcagccgat ctatcaaaca tgcgggcggt
    36901 taatggccgt ggagctatta acggcggtca cgcatccctt gaacccttcg caacgcctgc
    36961 cgccggatcg ctattttact gaaatcaccg tcagccatcg gatggaggtt gtgaaagagc
    37021 agattgattt gctggcgcaa aaagccgact tctttataga gcacggcctg ctggcatcgg
    37081 tcaatattga tggtcctacg ctcatcgccc tgcgtcagca accaaaaatc ctgcgccaga
    37141 ttgagcgtct tccctggctg cgtttcgaac tggtggagca tatccgtctg ccgaaagatt
    37201 caacctttgc ctcgatgtgt gaatttggcc cgctgtggct ggatgatttt ggtaccggga
    37261 tggcaaattt ctctgcacta agtgaagtgc gttatgacta catcaaaatc gcgcgagaac
    37321 tgtttgtgat gctgcgtcag tcgccggaag gacgcacact cttttctcag cttttacatc
    37381 taatgaatcg ctattgtcgc ggggtgattg tcgagggcgt agaaacgccg gaagagtggc
    37441 gtgatgtaca gaactcgccc gcattcgccg cacaaggctg gtttctttca cgcccggcac
    37501 cgatagaaac gctgaatacg gcggttctgg cgctataagc tgcctcattt tccgcctggc
    37561 tggactatct ttaggactgg cacaggaaga atatgaggaa gcgaacgatg agcaaggcag
    37621 gcaaaataac cgctgcgatt tcaggggctt tcttgttgtt gattgtcgtg gcgatcattt
    37681 tgattgcaac ttttgactgg aatcgactca aaccgaccat caaccagaaa gtctctgcgg
    37741 agttgaatcg tccgttcgct atccgtggcg atctgggcgt ggtgtgggag cggcaaaagc
    37801 aagaaactgg ctggcgcagc tgggtgccgt ggccccatgt acacgcggaa gacatcattc
    37861 ttggcaatcc accggatatt cccgaagtca cgatggtgca tttgccacgc gtagaagcaa
    37921 cgctggcccc gctggcgctg ctgaccaaaa cggtctggct gccgtggatc aagctcgaaa
    37981 agcccgacgc gcgcctgatt cgcctctctg aaaagaacaa taactggacg tttaatctcg
    38041 ccaacgatga taacaaagac gcgaatgcaa agccgtcggc atggtcgttt cggctggata
    38101 atattctttt cgatcaaggg cggatcgcca ttgatgacaa agtaagcaaa gcggatctgg
    38161 agatttttgt tgatccctta ggcaagccgc tgccgttcag cgaagttact ggatcgaaag
    38221 gtaaagcgga taaagaaaag gtgggcgatt acgtttttgg cctgaaggcg cagggacgtt
    38281 ataacggtga accgctcacg ggtacgggaa aaataggcgg tatgctggcg ctgcgtggcg
    38341 aagggacgcc gtttccggta caggctgatt tccgttcagg taatacccgt gttgcttttg
    38401 atggcgtcgt gaatgaccca atgaagatgg gcggtgtcga tttacggctt aaattttctg
    38461 gcgattcact gggtgatctc tatgaactga cgggcgttct gctgcccgat accccgccgt
    38521 ttgaaacgga tggtcggctg gtagcgaaaa tcgacactga aaaatcgtcg gtctttgatt
    38581 atcgcggctt taatgggcga attggcgata gcgatatcca cggttctctg gtctacacca
    38641 ccggcaagcc acgaccaaaa ctggaaggtg atgtcgagtc gcggcaattg cggctggcgg
    38701 acctgggacc gttgattggc gttgattccg ggaaaggggc agaaaagtcg aaacggtctg
    38761 aacagaagaa gggcgaaaaa agcgttcagc ctgcgggcaa agtgctgcct tatgaccgct
    38821 tcgaaaccga taaatgggac gttatggatg ccgatgttcg cttcaaaggg cggcgcattg
    38881 agcatggcag tagcctgccg attagcgatc tttctactca tatcatcctc aaaaatgctg
    38941 acttgcgcct gcaaccgctg aaatttggca ttgcgggtgg cagcattgcg gcgaatattc
    39001 atctggaagg cgataaaaag ccgatgcagg ggcgggcaga tattcaggct cgtcgactga
    39061 aactgaaaga actgatgccc gatgtggaac tgatgcagaa gacgctgggg gaaatgaacg
    39121 gtgacgcgga actgcgcggt agcggtaact cggtggcggc acttttaggc aacagtaacg
    39181 gcaacctgaa actgttgatg aatgacgggc tggtgagccg caacctgatg gagattgttg
    39241 ggctgaatgt cggcaactac attgtcggtg cgatatttgg tgacgatgag gtgcgggtga
    39301 actgcgcggc ggcgaatctg aatattgcca acggcgtggc acgcccgcag atttttgctt
    39361 tcgatactga gaacgcgttg attaacgtta ccggcacggc aagttttgct tcggaacagc
    39421 tggatttgac tattgatccg gagagtaaag gtattcggat tatcacactg cgttcgccgc
    39481 tgtatgtgcg tgggacgttt aaaaatcctc aggctggggt gaaagccggg ccgttgattg
    39541 cccgtggtgc tgttgctgcg gcactggcaa cgctggttac gccagcggca gcgttgctgg
    39601 cactgatctc accttccgaa ggggaggcta atcagtgccg gacgattttg tcgcagatga
    39661 agaagtgaag ataattgccg gatgtctgac atccggcaat taaacagatt tagattgttg
    39721 gcaaagtgtg ctttgttcat gccggatgcg gcgtgaacgc cttatccggc ctacaaatcc
    39781 ttgcgaattc aatatattgt atggaaattc aggcctgata agcgtagcgc atcaggcttt
    39841 tcactcttat tacaacgact gatgtcgcgt ctcatgggtc agcagcaggg cgattaacgt
    39901 caagccagcc atcgccgcca gatataaccc caccgcactt agcccgtagt tagtctgcaa
    39961 ccaggctgcg atatatggcg caacggaagc cccgagaatc gacgctacgt tgtaagagaa
    40021 cgatgctccg gtgtaacgca cttctgtcgg aaacagctct ggtaacagcg cacccattgg
    40081 cccgaaggtc agacccatca gacttaaccc cagcagcagg aaggcaaaaa ccagaatcgg
    40141 gttgccagaa ccgagcagtg ggttaaaggc gaacagcgcg aacaggatga tcagcgtggt
    40201 gatgattacc atgcttttac ggcgaccaaa ggcatcagcc agtaatccag cgactggcac
    40261 catcacgcca aaaccaataa ctgccatcat caacatccac aacacttcgt tacgcggcag
    40321 gccaagccca actggcgcgg cggcggtact aaaggtcata gagtagaccg tcatgatgta
    40381 aaacagcgta taggttgcca gcataatgaa cgtacccagt acggttacgc gaacatgttt
    40441 ggtcagcagc gtacccagcg ggatcttcac ctgttttttc gctttagcga ctttctcaaa
    40501 caccggcgac tcatgcagcg acacgcgaac atacaggccg ataatgacca gcaccgccga
    40561 gaagataaat ggcacacgcc agccccagct cataaactgc tcgtcggtca gtagccagga
    40621 aagcagcaag aaagtgccgt tggcaaagaa gaagccaatc ggtgcgccca gctgcgggaa
    40681 ggagccgtac aatgcacgtt tgcgcggtgg ggcgttttca gtcgccagca gcgccgcgcc
    40741 gccccattca ccacctaagc ccagaccctg accaaatcga gccagcgcca gcagcagcgg
    40801 ggcgaaaata ccaatcgtgg catagcctgg cagcagacca atcaccacgg tcgaaatccc
    40861 catcgttagc aacgaggcga ccagcgtcgc tttacgccca acgcgatcgc caaaatgacc
    40921 aaaaacggca gagccaatgg ggcgcgcgac gaaggcgatg gcgaaggtgg cgagcgactg
    40981 tagcgttgct gccgtaggat cgccctgcgg gaagaagata tgcggaaaca caataacggc
    41041 cgcagtggcg taaatgtaaa agtcgaagaa ctcaatggcc gtgccaatga gagaggcgac
    41101 aaggacttta ttacgcgagt tgatcggcgt gtattcttgc tcgtggtcga gtgttgtggc
    41161 tgttgcttgc ataatctttt cttatttttt ggctaacgaa tagccatatt acgcacagca
    41221 aaagtgatat ttcaatgtgt aacaaatcgc ggaaatggtc aaaaaccagg caaaaagggg
    41281 ataatgttta ggccagtgat ttttcttcta tgaaatgctt cacataattt tataaatagt
    41341 ggataaaact ggtttcaggt taaataaaag ttaccaactg gatttatatg ctggggttat
    41401 gagttggcat gaaattgttt gctcctgaat agatggctga agaggctctg ggaaagagtt
    41461 tcaagaggtt atgtgcgcat tatagccgga tgcgatgctg gcgcatctta ttcggcctac
    41521 ggggagccag gccgaataaa ttcgtatgcc ggataaggcg ttttgaactg catccggcct
    41581 attttaaggc tgcgttttcc ccggcattcg cgggtcgtct ttatattcgg cggtggcaat
    41641 ccacgccgcg caaaacagcg tcaaacgggc gaagaagtag aaaaatgcca tcagccccag
    41701 cacggaacca aatgctgcgc cagaggggga tttcatcaac gatggcaggg tgtaggtcat
    41761 cacgatttta atcacttcaa aaccaatagc agccagaaat gttccgcgaa tcagggcttt
    41821 tttgcgcgga cggtggcgcg gcaaccgcca gaagatccag aagaaaagca gatagttggc
    41881 gaagatggaa atcgccaggc caatcaaccg ccacgtcggt ttcagccact caatgctgtt
    41941 caggtgcagg gcgctaataa tcatttgctg cgccgaaccg gcaaccgagg tgatcgaaag
    42001 cgtcacaatc agcgcaatca acaaaccaat cagcgaaata aaatcacgca gatattttac
    42061 ccagaacttc tcctgatctt gcggcgagcg ttcccagaca tcgcgcgact gggcacgaat
    42121 cgcttcacgc aggttaccca tccagttgat gccggaataa agcgccaccg ccaggccgac
    42181 aagccctaca gtcgtacgct gctgaacggc ggtgttgatg gtgtttttca acgtagcggc
    42241 tagcgtcgga tcgctgatgt tttgcagaat tttgtcgaag atatcctgta gcaacatcgg
    42301 atgggaggcc agcacaaagc cccctgcggc aaacgacacc atcaaaatcg ggatcatcga
    42361 caaaaatgag aaataggtga tagccgcacc aaactggtta cccagccgat cgttaaagcg
    42421 ttctgtcgcg cgaatcaggt gcgcaataac cggttggcgt tggacttttt cggcagtggt
    42481 ggtgacggtt tccagcgcct ggctaacttt gccgcctttc tcgctattat ccaactgctt
    42541 aatcggctcg tgctccagat cctgggtggg acgtttgatc tcgttttcct gcgtcatccg
    42601 ctcttttgtc cttctgtcgt ggtcattctt aaaaagtata gtcggtcagt ccaggtactc
    42661 tttcattacg ccagccagcc actgcataaa caggtttaca cgccgggaaa gctcccgacg
    42721 ctgcggataa accagcgaaa gggagagcgg ctcggcacgg tagcctggca acacttcaat
    42781 aagccgcccg gcacgcaggg cttcgcgcac ggcaatgcgc gggatctgaa taatccccag
    42841 accggcaaga ccggcggcga gatagttttc gctactgttt accgtcaaca tgccgccaga
    42901 cttaaaccac tggacgccat taacgctggc aacctcaaaa cctaacggat gtacacccag
    42961 gtgcggtgta taacgcacta ttgcgtgtga agtgagatcg tcggggtttt gcggataacc
    43021 aaagcgcgtc agatagtgcg gactggcaca gttgaccatg gtcagtttgc cgaggggacg
    43081 ggcgataacg ccatcttccg gtaacgcgcc agtgcgtatc acgcaatcaa aaccatcatg
    43141 aagaatatct accggacggt catggctact cagttccagc tcaattcccg gatgctgata
    43201 gagaaattcc gacaggtgcg gcagtaacag gctttttgcg attcctggcg ggatgtcgat
    43261 gcgtaattta ccgctgatac tggtggcatc ctgctgaaac agaccgtcca gttcgttgag
    43321 attactcaac acatcttttg ctcgttgata ataggtcatg ccttctggcg tgagtttgac
    43381 ccgtcgcgtg gtgcgctgga gaagctgggt gccaagttga tgttccagtg cctgtatctg
    43441 gcgcgaaaca cttccttttg gcaaagcaaa gaaatccgct gcgcgggaaa aactttccag
    43501 ctccgcgact ttgatgaaca actgcattgc gtgaatttta tccatttctt tgaccgattg
    43561 ttgttcacag cgaaacagta acgcgcatat cgcattgttt attatttttt ctaacatcta
    43621 atgccgggtt tcacaatgtt gtcatgacga atgtggcgat ttctcagcag gaaaagggcg
    43681 gaatagcttt ctgagaaagg tgaatcccct taaacagagc gtatttttca gcgattccct
    43741 ttaaagtgaa tagaggacat attggctatt tgtgcttatt tttgtctcat tgccatttgt
    43801 tattttggct tgtggtcgcg gaatttcatt tttaattgca tgcgtactaa ccaccaatat
    43861 aaggaaatcc tcaaaattgt tttaaatagt gccctcgaca tgtaatttca ggcgtttagc
    43921 tgtaactatt tatatcggta actattttct acgcgccatc tctgtagaat ggccaccgcc
    43981 aaatggcgaa ttcttatttt ttctcgcggg taataccctt ccactactta agtcttttta
    44041 acgtattcag gtggagattc agttatgcaa atagtcatgt ttgacaggca gtcaatattt
    44101 attcatggaa tgaaaatcag tttacagcag cgtattccag gagtgagtat tcagggggcc
    44161 agtcaggcag acgagttatg gcaaaagctg gaaagttacc ctgaagcctt agttatgctc
    44221 gatggcgatc aggatggtga gttttgctac tggttgctgc aaaaaaccgt ggtgcaattt
    44281 cctgaggtta aggtgttaat tacggcgacg gactgcaata aacggtggtt acaggaagtt
    44341 atccatttta atgtgctggc cattgtgcct cgtgattcaa ccgtcgagac atttgcgttg
    44401 gcggtaaata gtgcggcgat ggggataatg tttctgcctg gagactggcg gacgacgccg
    44461 gaaaaggaca ttaaagatct caaatcgtta agtgcccgcc agcgggagat tttaaccatg
    44521 ttagcggcgg gcgaatcaaa taaggagatc ggcagagcgc tgaatatcag taccggaacg
    44581 gtaaaagcgc atctggaatc cctgtatcgc cgtctggaag tgaaaaatcg cacccaggcg
    44641 gcaatgatgt taaatatctc ctcctgaaga ggataatttt acagccagca ggaagttagc
    44701 ggctggctgt aaaaatatta tggttcgccg tacaaaccaa ttaaacggcg taccacaccg
    44761 ttagtccagc caaacccatc ctgcaacgga tactcgccac cgccgccttc gcggggaaca
    44821 ccatcggcaa tatggtattt ttcgatcagt ttgtgctgtt ccagataaaa ctgattcacc
    44881 gtcttcagcc agctgcgcgc gatttcatcg cccagaaggt catcgccata cattttaaat
    44941 ccctgaattg ccatccattg taacggtgcc cagccgttgg gtttatccca ctgttcaccg
    45001 gtttcgtact cgcttgccag aatcccgcca ggtgtcagta accgactgcg cacggcgttt
    45061 gccagacgat cggcctgttc atggttcgcc ataccgacat agagcggcac aatggcggca
    45121 gcggaaaaca gcgccagttg ttcgcgtcgc cagtcgtaat cgcggtagat gccgttttca
    45181 tcatcccaga ggtaacggtt taccgcatcg cgacgggcac tggccttctg gcggaacagt
    45241 gcttctgtct ctttctcacc tttcagcgcc gagatgttgg cgatggcgct ctccagttta
    45301 aacaggaagg cattcagatc gatggggatg aactgggtgg tacgaatgct cgccagacga
    45361 ccagtatcac ggagccaacg ggaagagtaa tcccaaccgg aggccgcccc cgcgcgtaaa
    45421 tcgcggtaca cctcgttggg cgggcgacca gaatgtttcg cggtttcaac gtcctcaagc
    45481 caggattcgt cacgcggcgt gtcgcgatcg tcccagtaac ggttgagcag cgatccgtcc
    45541 ggcatccgca caacatggcg ataggcctga ttagggatca acgattctgc accgtccatc
    45601 cagaaggcat attccatttt caggtggtcg agatagcggc gcgcaccgcg tacaccatct
    45661 tcttcaaaca actccaccat cagcgcaaaa accggtggtt gcgagcggct caaataatag
    45721 gtgcggttgc cgttggggat gtgaccgtag ttttcgatca tccaggcgaa gttatcggcc
    45781 atgcatttca gcaaatcttc ccgaccactt tccgccagcc ccagcatggt gaaataggaa
    45841 tcccaatagt aggtttcgct aaaacggccg cccgggacaa tatatgactg cggcagcgcc
    45901 agcagagaag accacggaat gtgatcctgt ggttcgcggg ttagcaccgg ccacagctgg
    45961 tcgatatgct ctttcaggga attttgcggg tccgatacat actcgctgga gtagacctcc
    46021 ggcagccaga agtgattttc aacaaacttg cgcaagtcaa aatcacgatg acggcgcact
    46081 ttacggtagc ggattaaaat atccagcggg tccattttgg gtgcgcagtc gggaaaggtt
    46141 ttactgtcgg ggaaaatttt cgccgactga acatgctcga acagttcgag atagcgatcg
    46201 gcaggcgtca gcgcatcaga ggcgggcagc ccttcaatca tctcgggttc cggttctgcc
    46261 tcgatcatct catccagttt taattcatac gggtccagtt catagcagag atcgacctcg
    46321 atcatcagtt cgtctggatt agggttttga attttctgat tgagcataag gatccaacct
    46381 ccgaaattcg tcgctaatta tcgggctttt ctttaagggt agtcattcgc tccgcctggt
    46441 gggcgacaaa gcgtgcggtg gatcacgttt attaacctgt caaaaaatat aacgcactaa
    46501 tatcaatgga atttataaaa atacagattt cgtgttgttt ttcattttca gaacattcaa
    46561 tcctgctctt gccgcaatca tgatatgcca ttcctgatct gggtaatagg cacaggctat
    46621 cttattgata gtttatattc atgtaattga ttgattccta tcaaaaagcg tatctccctg
    46681 ttttttatga tggctttgtc tcgcagagac gtttgcagca ttctggaaaa ttttctgggt
    46741 cacaataact caccaccgtg agggaagaat aatgaaaatg gtctcacgta ttaccgcgat
    46801 cggcctggct ggcgtcgcga tttgctattt agggttatct ggttatgtgt ggtaccacga
    46861 taataaacgc agtaaacagg ccgatgttca ggcatctgct gtcagtgaaa ataataaggt
    46921 tttaggcttt ctccgcgaaa aaggatgcga ctattgccac acgccttcgg cagaattacc
    46981 cgcctattat tatattcctg gcgcgaaaca gttgatggat tacgacatta agcttggata
    47041 taaatctttt aacctcgagg ccgtgcgtgc tgctctgctg gctgataaac ccgtttcgca
    47101 aagcgatctg aataagattg aatgggtgat gcagtatgaa actatgccgc caacgcgtta
    47161 taccgcgcta cactgggcgg gtaaggtgag tgatgaagag cgggcggaaa tactggcctg
    47221 gattgcaaaa cagcgcgcgg aatattacgc cagcaatgat actgctccgg agcatcgcaa
    47281 tgaaccggtg cagcccatcc cgcaaaaact gcctaccgat gcgcaaaaag tggcgttggg
    47341 ctttgcgctg tatcacgatc cccgtttatc ggctgatagc accatttcat gcgctcattg
    47401 ccatgcgttg aatgcggggg gcgtcgatgg cagaaaaaca tcgattggtg ttggtggcgc
    47461 agttgggccg attaacgcgc cgacggtatt taactcagta tttaacgttg agcagttctg
    47521 ggatggtcgt gcggcaacat tgcaggatca ggctggtgga ccgccgttga acccgattga
    47581 aatggcgtca aaatcctggg acgaaattat tgctaagctg gaaaaagatc cgcagcttaa
    47641 agcgcagttc ctcgacgtct atccgcaagg tttcagtggc gaaaatatta ctgatgccat
    47701 tgctgaattt gagaaaacat taattacgcc ggattcccca tttgataaat ggttgcgtgg
    47761 ggatgaaaat gcgctgacgg cgcaacagaa aaaaggctat caattattta aagataataa
    47821 atgtgcaact tgtcatggtg gaattattct cggcggacgt tccttcgaac cgttggggct
    47881 gaaaaaagac tttaactttg gggaaattac ggcggcggat attggtcgta tgaatgtgac
    47941 taaagaagag cgtgataaat tgcgtcagaa agtacccggt ttacgtaacg ttgctttaac
    48001 ggcaccgtac ttccatcgcg gtgacgtgcc gacgctggac ggggcggtga aactgatgtt
    48061 gcgctatcag gtaggcaaag agctgccgca ggaggatgtg gatgatatcg tagctttcct
    48121 gcacagtctg aacggggttt acacgccgta tatgcaggat aaacaataat taatttgatc
    48181 gcccgaacag gaatgtttgg gcgattttta tt
//
LOCUS       DBFIFI010000014        46832 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.014, whole genome shotgun sequence.
ACCESSION   DBFIFI010000014 DBFIFI010000000
VERSION     DBFIFI010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 46832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46832
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.014"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            3..587
                     /gene="yraH"
                     /locus_tag="N7B39_000811"
     CDS             3..587
                     /gene="yraH"
                     /locus_tag="N7B39_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2420839.1"
                     /translation="MNKVTKTAIAGLLALFAGNAAATDGEIVFDGEILKSACEINDSD
                     KKIEVALGHYNAEQFRSVGDRSPKIPFTIPLVNCPVTGWEHDNGNVEASFRLWLETRD
                     NGTVPNFPNLAKVGSFAGTAATGVGIRIDDAESGNLMPLNAMGNDNTVYQIPADSAGI
                     VNVDLIAYYVSTVEASEITPGEADAVVNVTLDYR"
     gene            667..1362
                     /gene="yraI"
                     /locus_tag="N7B39_000812"
     CDS             667..1362
                     /gene="yraI"
                     /locus_tag="N7B39_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HGU2420840.1"
                     /translation="MSKRTFAMIFTLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGTSRAPFIITPPLIRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVSDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKADLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1391..3907
                     /gene="yraJ"
                     /locus_tag="N7B39_000813"
     CDS             1391..3907
                     /gene="yraJ"
                     /locus_tag="N7B39_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HGU2420841.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYNFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWDYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDDDNNHN
                     SSVNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            3918..4651
                     /locus_tag="N7B39_000814"
                     /pseudo
     CDS             3918..4651
                     /locus_tag="N7B39_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            complement(4694..5554)
                     /gene="rsmI"
                     /locus_tag="N7B39_000815"
     CDS             complement(4694..5554)
                     /gene="rsmI"
                     /locus_tag="N7B39_000815"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HGU2420842.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            5619..7655
                     /gene="lpoA"
                     /locus_tag="N7B39_000816"
     CDS             5619..7655
                     /gene="lpoA"
                     /locus_tag="N7B39_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HGU2420843.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFVGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADGVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            7613..8008
                     /gene="yraN"
                     /locus_tag="N7B39_000817"
     CDS             7613..8008
                     /gene="yraN"
                     /locus_tag="N7B39_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HGU2420844.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEVQARRWLEGKGLRFVAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDTFNDHS"
     gene            8028..8618
                     /gene="diaA"
                     /locus_tag="N7B39_000818"
     CDS             8028..8618
                     /gene="diaA"
                     /locus_tag="N7B39_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HGU2420845.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            8628..9203
                     /gene="dolP"
                     /locus_tag="N7B39_000819"
     CDS             8628..9203
                     /gene="dolP"
                     /locus_tag="N7B39_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HGU2420846.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(9317..10357)
                     /gene="yraQ"
                     /locus_tag="N7B39_000820"
     CDS             complement(9317..10357)
                     /gene="yraQ"
                     /locus_tag="N7B39_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HGU2420847.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(10430..11065)
                     /gene="yraR"
                     /locus_tag="N7B39_000821"
     CDS             complement(10430..11065)
                     /gene="yraR"
                     /locus_tag="N7B39_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HGU2420848.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            11193..11711
                     /gene="yhbO"
                     /locus_tag="N7B39_000822"
     CDS             11193..11711
                     /gene="yhbO"
                     /locus_tag="N7B39_000822"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HGU2420849.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(11691..12134)
                     /gene="yhbP"
                     /locus_tag="N7B39_000823"
     CDS             complement(11691..12134)
                     /gene="yhbP"
                     /locus_tag="N7B39_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HGU2420850.1"
                     /translation="METLTAISRWLAKQHVVTWCVQQEGELWCANAFYLFDVQKVAFY
                     ILTEEKTRHAQMRGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRPDEIKFTDNTLGFGKKMIWLRGSGTEQA"
     gene            12185..12487
                     /gene="yhbQ"
                     /locus_tag="N7B39_000824"
     CDS             12185..12487
                     /gene="yhbQ"
                     /locus_tag="N7B39_000824"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HGU2420851.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAKLLSSLQTPEIKSD"
     gene            complement(12474..12977)
                     /gene="yhbS"
                     /locus_tag="N7B39_000825"
     CDS             complement(12474..12977)
                     /gene="yhbS"
                     /locus_tag="N7B39_000825"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU2420852.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(12971..13495)
                     /gene="ubiT"
                     /locus_tag="N7B39_000826"
     CDS             complement(12971..13495)
                     /gene="ubiT"
                     /locus_tag="N7B39_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HGU2420853.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            13704..14699
                     /gene="ubiU"
                     /locus_tag="N7B39_000827"
     CDS             13704..14699
                     /gene="ubiU"
                     /locus_tag="N7B39_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HGU2420854.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            14708..15586
                     /gene="ubiV"
                     /locus_tag="N7B39_000828"
     CDS             14708..15586
                     /gene="ubiV"
                     /locus_tag="N7B39_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HGU2420855.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            15667..16674
                     /gene="yhbW"
                     /locus_tag="N7B39_000829"
     CDS             15667..16674
                     /gene="yhbW"
                     /locus_tag="N7B39_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HGU2420856.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSARDAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVAWFDARDPNPHVRPVPG
                     YGEQIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(16791..18035)
                     /gene="mtr"
                     /locus_tag="N7B39_000830"
     CDS             complement(16791..18035)
                     /gene="mtr"
                     /locus_tag="N7B39_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HGU2420857.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIDIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(18189..20078)
                     /gene="deaD"
                     /locus_tag="N7B39_000831"
     CDS             complement(18189..20078)
                     /gene="deaD"
                     /locus_tag="N7B39_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HGU2420858.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFSGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(20071..20151)
                     /gene="yrbN"
                     /locus_tag="N7B39_000832"
     CDS             complement(20071..20151)
                     /gene="yrbN"
                     /locus_tag="N7B39_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HGU2420859.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(20258..21142)
                     /gene="nlpI"
                     /locus_tag="N7B39_000833"
     CDS             complement(20258..21142)
                     /gene="nlpI"
                     /locus_tag="N7B39_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HGU2420860.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(21251..23386)
                     /gene="pnp"
                     /locus_tag="N7B39_000834"
     CDS             complement(21251..23386)
                     /gene="pnp"
                     /locus_tag="N7B39_000834"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HGU2420861.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(23633..23902)
                     /gene="rpsO"
                     /locus_tag="N7B39_000835"
     CDS             complement(23633..23902)
                     /gene="rpsO"
                     /locus_tag="N7B39_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HGU2420862.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTRLIERLGLRR"
     gene            complement(24051..24995)
                     /gene="truB"
                     /locus_tag="N7B39_000836"
     CDS             complement(24051..24995)
                     /gene="truB"
                     /locus_tag="N7B39_000836"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HGU2420863.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDVDGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(24995..25396)
                     /gene="rbfA"
                     /locus_tag="N7B39_000837"
     CDS             complement(24995..25396)
                     /gene="rbfA"
                     /locus_tag="N7B39_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HGU2420864.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(25561..28233)
                     /gene="infB"
                     /locus_tag="N7B39_000838"
     CDS             complement(25561..28233)
                     /gene="infB"
                     /locus_tag="N7B39_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HGU2420865.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(28258..29745)
                     /gene="nusA"
                     /locus_tag="N7B39_000839"
     CDS             complement(28258..29745)
                     /gene="nusA"
                     /locus_tag="N7B39_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HGU2420866.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQVQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(29773..30225)
                     /gene="rimP"
                     /locus_tag="N7B39_000840"
     CDS             complement(29773..30225)
                     /gene="rimP"
                     /locus_tag="N7B39_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HGU2420867.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(30432..30508)
                     /locus_tag="N7B39_000841"
     tRNA            complement(30432..30508)
                     /locus_tag="N7B39_000841"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30472..30474),aa:Met,seq:cat)
     gene            30856..32199
                     /gene="argG"
                     /locus_tag="N7B39_000842"
     CDS             30856..32199
                     /gene="argG"
                     /locus_tag="N7B39_000842"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HGU2420868.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSAASGVPQMENLENKGQ"
     gene            complement(32207..33832)
                     /gene="yhbX"
                     /locus_tag="N7B39_000843"
     CDS             complement(32207..33832)
                     /gene="yhbX"
                     /locus_tag="N7B39_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HGU2420869.1"
                     /translation="MTVFNKFARSFKSHWLLYLSVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVAKMLGMYSPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKNAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQSQTLEEVIVHYKGDS
                     LVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(34291..34377)
                     /locus_tag="N7B39_000844"
     tRNA            complement(34291..34377)
                     /locus_tag="N7B39_000844"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34341..34343),aa:Leu,seq:gag)
     gene            complement(34392..34724)
                     /gene="secG"
                     /locus_tag="N7B39_000845"
     CDS             complement(34392..34724)
                     /gene="secG"
                     /locus_tag="N7B39_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HGU2420870.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(34952..36289)
                     /gene="glmM"
                     /locus_tag="N7B39_000846"
     CDS             complement(34952..36289)
                     /gene="glmM"
                     /locus_tag="N7B39_000846"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HGU2420871.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVETALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(36282..37130)
                     /gene="folP"
                     /locus_tag="N7B39_000847"
     CDS             complement(36282..37130)
                     /gene="folP"
                     /locus_tag="N7B39_000847"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HGU2420872.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(37220..39154)
                     /gene="ftsH"
                     /locus_tag="N7B39_000848"
     CDS             complement(37220..39154)
                     /gene="ftsH"
                     /locus_tag="N7B39_000848"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HGU2420873.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNAGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(39254..39883)
                     /gene="rlmE"
                     /locus_tag="N7B39_000849"
     CDS             complement(39254..39883)
                     /gene="rlmE"
                     /locus_tag="N7B39_000849"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HGU2420874.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            40009..40302
                     /gene="yhbY"
                     /locus_tag="N7B39_000850"
     CDS             40009..40302
                     /gene="yhbY"
                     /locus_tag="N7B39_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HGU2420875.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(40458..40934)
                     /gene="greA"
                     /locus_tag="N7B39_000851"
     CDS             complement(40458..40934)
                     /gene="greA"
                     /locus_tag="N7B39_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HGU2420876.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            41182..42615
                     /gene="dacB"
                     /locus_tag="N7B39_000852"
     CDS             41182..42615
                     /gene="dacB"
                     /locus_tag="N7B39_000852"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HGU2420877.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAVAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(42655..43827)
                     /gene="cgtA"
                     /locus_tag="N7B39_000853"
     CDS             complement(42655..43827)
                     /gene="cgtA"
                     /locus_tag="N7B39_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HGU2420878.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKVEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(43843..44808)
                     /gene="yhbE"
                     /locus_tag="N7B39_000854"
     CDS             complement(43843..44808)
                     /gene="yhbE"
                     /locus_tag="N7B39_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGU2420879.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLKSLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(44935..45192)
                     /gene="rpmA"
                     /locus_tag="N7B39_000855"
     CDS             complement(44935..45192)
                     /gene="rpmA"
                     /locus_tag="N7B39_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HGU2420880.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(45213..45524)
                     /gene="rplU"
                     /locus_tag="N7B39_000856"
     CDS             complement(45213..45524)
                     /gene="rplU"
                     /locus_tag="N7B39_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HGU2420881.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            45783..46754
                     /gene="ispB"
                     /locus_tag="N7B39_000857"
     CDS             45783..46754
                     /gene="ispB"
                     /locus_tag="N7B39_000857"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HGU2420882.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLKPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
BASE COUNT        11576 a        12681 c        11414 g        11161 t
ORIGIN      
        1 aaatgaataa agttacaaaa acagctattg cgggcttact tgcgcttttc gcaggtaatg
       61 ccgctgcaac cgatggtgaa atagtttttg atggcgagat tttaaaatcc gcttgtgaaa
      121 tcaatgactc tgataagaaa attgaagttg ctcttggtca ctataatgcc gaacagtttc
      181 gttctgtagg cgatcgtagc ccaaaaattc cattcactat tcctttagtt aactgcccag
      241 tgactgggtg ggagcacgat aacggcaacg ttgaagcgtc tttccgttta tggctggaaa
      301 cgcgggataa tggtaccgtc cctaacttcc ctaacttggc aaaagtgggc tcttttgctg
      361 gcacagcagc aacaggtgtg ggtatccgta ttgacgatgc ggaaagcgga aaccttatgc
      421 cactaaatgc tatgggcaat gataacacag tttatcagat ccctgcggac tccgctggaa
      481 ttgtcaatgt tgacctcatc gcttactacg tatcaaccgt tgaagcatcc gaaatcaccc
      541 caggggaagc agacgctgtc gttaacgtaa cgctggatta ccgttaatac gttacggcgt
      601 tatctgacct ttcagataac gcccttttcc ttcctatttc tcgttgtatc aggttgaaaa
      661 ataactatgt caaaacgaac attcgcgatg atatttacct tgttgtgtag cttctgtatc
      721 ggccaggcgc ttgcaggggg tatcgtttta cagcgaacgc gagtgatcta tgatgccagc
      781 cgcaaagagg ctgcgttacc tgtcgcaaac aaaggcgcag aaacacctta tttactgcaa
      841 tcatgggtag ataatataga tggtacaagc cgtgccccat ttattataac cccaccgcta
      901 attcgtcttg aagctggcga tgattcatca ctgcgaatta ttaaaacagc tgataacctg
      961 cctgaaaata aagagtcgct gttctacatt aatgttcgtg ccattccagc aaagaaaaaa
     1021 tcagatgatg ttaatgctaa cgagttgacg ctggtattta aaacacggat caaaatgttt
     1081 tatcgccccg cacacctgaa gggacgggta agcgatgcgt ggaaatcact ggaatttaaa
     1141 cgtagtgatc attcactcaa tatatataac ccaactgaat attacgtcgt atttgccgga
     1201 ctagcagtcg ataaagccga tctcacaagc aaaattgaat atatcgcgcc cggagaacat
     1261 aaacagttac cacttccagc atctggcgga aagaacgtga aatgggctgc gatcaatgat
     1321 tatggcggca gttccgggac agaaactcgt ccactgcaat aacaaatata aaaacacagg
     1381 tcatcaggga atgccacaac gacaccacca gggacataaa cgcacaccga aacagttggc
     1441 gctcattatc aaacgctgtt tgccgatggt gctcactggc agcggcatgc tttgcactac
     1501 cgctaacgcc gaagagtatt atttcgaccc cattatgctg gaaaccacaa aaagtggtat
     1561 gcaaacaacc gatctgtcac gtttttcaaa aaaatacgca caactaccag gaacttatca
     1621 ggttgatatc tggctgaata aaaagaaggt ttcacagaaa aaaattacat ttaccgccaa
     1681 tgcagagcaa cttctgcagc cacagtttac ggtagaacaa ctacgtgagc tgggtattaa
     1741 ggtggatgaa atcccggcgc tggctgaaaa agatgacgat agcgtgatca actcgcttga
     1801 acaaatcatt cccggtacag ctgctgaatt tgatttcaat catcagcgac ttaatttgag
     1861 cattccccaa attgcactgt accgtgatgc aagaggttac gtctcccctt ctcgttggga
     1921 cgatggtata ccaacgctgt ttaccaacta ctcgtttaca ggttctgata accgttaccg
     1981 ccagggcaat cgtagccaac gacagtacct aaatatgcaa aatggtgcca attttggccc
     2041 ctggcgatta cgtaactatt ctacgtggac acgcaacgat caggcgtcaa gctggaacac
     2101 tatcagtagt tatttacaac gtgatatcaa ggcgttgaag tctcagttgc ttctgggaga
     2161 aagcgccacc agcggcagta ttttttccag ctacaacttt actggcgtgc aactcgcttc
     2221 cgacgataat atgttgccaa acagccagcg cggatttgcc ccaacggtac gcggtatcgc
     2281 aaacagtagt gcaatcgtga ctatcaggca aaatggttat gtgatctatc aaagcaacgt
     2341 gccagcgggt gcctttgaaa ttaacgatct ctacccctct tccaacagcg gcgatttaga
     2401 agtcacgatt gaagaaagtg acggtacgca acgtcgcttt atccagcctt attcttcatt
     2461 acccatgatg cagcgacctg ggcatctaaa atatagcgcg accgctggac gctatcgcgc
     2521 tgatgcaaac agtgatagca aggaacccga atttgctgaa gccacggcaa tatatggttt
     2581 gaataatact tttacgctgt atggcggcct gctcggttct gaagattatt atgcgctggg
     2641 gatcggtatc ggcggcacac ttggcgcact gggcgcgttg tcgatggata tcaacagagc
     2701 tgacacccaa ttcgataacc agcactcttt tcatggctat caatggcgta cgcagtacat
     2761 caaagatatc ccggaaacca acaccaatat cgctgtcagc tactatcgct ataccaacga
     2821 tggctatttt agttttgatg aagccaatac ccgcaattgg gactataaca gtcgccaaaa
     2881 aagtgaaatt caattcaaca tcagccagac aatatttgat ggggtaagtc tgtatgcctc
     2941 cggttcacag caagactatt ggggcaataa cgagaaaaac aggaatatct ctgttggggt
     3001 ttccggccag caatggggaa ttggttacag cctgaattat caatacagcc gctacactga
     3061 tcaaaataat gaccgcgcac tctctttgaa tctcagtatt ccgttagaac gctggttacc
     3121 gcgtagccgg gtttcctatc agatgaccag ccagaaagat cgcccaaccc aacatgaaat
     3181 gcgtcttgat ggctcactgc tggatgatgg tcgcctgagc tatagtctgg aacaaagtct
     3241 ggatgacgat aacaaccata acagtagcgt gaacgccagt taccgttcac cttatggaac
     3301 cttcagtgcc ggatacagtt acggtaatga cagtagccaa tacaattacg gcgttaccgg
     3361 cggcgtggtt atccatcctc atggtgtgac actctcgcaa tatctgggca acgcttttgc
     3421 gcttattgat gctaacgggg catctggcgt gaggatacaa aactatccgg ggattgctac
     3481 tgatcccttt ggctatgcag tggttcctta tctcacaact tatcaggaaa accgtctctc
     3541 ggtagatact acgcagctgc ccgataacgt cgatcttgaa caaacaacac agtttgtggt
     3601 gcccaacaga ggtgcaatgg tagcggcgcg tttcaacgcc aatatcggtt atcgcgtact
     3661 tgttacagtc agcgatcgca acggtaaacc gttgcccttt ggcgctcttg ccagcaacga
     3721 tgatacgggg caacaaagta tcgtcgatga gggcggcata ctatatctct ctgggatatc
     3781 gagtaaatca caaagctgga ctgtacgctg gggaaatcag gcagatcaac aatgtcagtt
     3841 tgcttttagt acaccggatt cagaaccaac aacctctgta ttacaaggca cagcgcagtg
     3901 ccattaagga taaaaaaatg aaaagagcgc ctcttataac aggacttttg ttgatatcca
     3961 catcctgcgc ttatgcctcc tcagaagggt gtggagctga cagcactagc ggtgcgacaa
     4021 attacagcag tgtggttgat gatgttacgg tgaaccagac agataacgtg acaggacggg
     4081 agtttacctc tgcaacgcta agtagcacta actggcaata cgcctgttcc tgctctgcgg
     4141 gtaaggcagt taaacttgtc tatatggtca gccccgtact taccaccact ggacatcaga
     4201 caggatatta caaactcaat gacagcctgg atattaaaac catgttatta aggttaattt
     4261 tggattcatc aatggtcaga agtttaccac ccgcaatgcc atgccagacg gttttactcc
     4321 agtagacttt gatatcactt atgactgtgg tgatacttca aagattaaaa actcgttgca
     4381 aatgcgcatc gacggtacaa ctggggtagt agaccagtac aacctggtcg ccagacgaag
     4441 aagttcagac aatgcgcccg atgtcggtat tcgtattgaa aatctcggcg gcggagttgc
     4501 aaatattcct tttcagaacg gtatccttcc cgttgatcct tccgggcatg gcaccgtcaa
     4561 tatgcgcgcc tggccagtta atctggtcgg tggtgagctg gaaacaggaa aatttcaggg
     4621 cacagccacc attaccgtca tcgtgcggta aaagaagacg ttgcgttttg attaacgcaa
     4681 cgcttcggca ctcttacccc tgctgctcca gcgcatactt atacagcgca tttttcttca
     4741 cgccgtgaat ttctgcggcc agcgccgccg cttttttcag cggcagttct gcctgtagca
     4801 gcgccagcgt gcgcagggca tcggcgggta agtcttcttc ctgtgcttta tgaccttcga
     4861 caatcagcac catttcgcct ttgcgacggt tttcatcttc ctttacccac gccagcagct
     4921 cgccaacggg cgcgccgtga atggtttccc aggttttggt cagctcacgc gccagaacca
     4981 cgtagcggga ttcgcctaat accgcaacga tatcttccag gctatctaac agacggtggg
     5041 tagattcata aaaaatcagc gtgcgcggct ccgcttcaat ggcttttagc gcatcacggc
     5101 ggccttttga tttggcaggt aaaaagcctt cgtaacagaa acggtcagag ggtaaacccg
     5161 cggcgcttaa cgcagtgata gcagcacacg gtccgggtag cggcaccaca cgaatccccg
     5221 cttcacggca ggtacgcacc agatggtagc caggatcgtt aattagcggc gttccggcat
     5281 cggaaaccag cgcaatgttt tgcccctctt gcagcttcgc cagcagcgtt tcggcttttt
     5341 gttgttcgtt atggtcgtgc agcgcaaaca accgggcatt aatcccaaaa tgttgcagca
     5401 ataaaccggt gtgacgagta tcctcggcgg caatcagatc aacggcctgt aatacctcta
     5461 acgcacgctg ggtgatatcc gccagattgc cgattggcgt cggtacaatg taaagctggc
     5521 cctgagaatt atccgccgat tggtgttgtt tcattgtgtc gtccgtattg ccgatttaat
     5581 attgagcatt gcgtaaaaaa aatatcactg gatacattat ggtaccctca acattttctc
     5641 gtttgaaagc cgcgcgttgt ctgcctgttg ttctggcagc cctgattttc gtcggttgtg
     5701 gcacccatac tcccgatcag tccactgctt atatgcaggg cacggcgcag gctgattctg
     5761 ccttttatct tcagcagatg cagcaaagct ctgatgatac caggatcaac tggcaattac
     5821 tcgccattcg tgcactggtg aaagaaggta aaaccgggca ggcggttgag ttgtttaacc
     5881 aactaccgca agaactgaac gattctcagc gtcgcgagaa aacactgctg gcggtagaga
     5941 ttaaactggc gcagaaagat tttgctggcg cgcaaaactt gctggcgaaa attacacccg
     6001 ccgatttaga acaaaaccag caagcccgtt actggcaggc aaaaatcgat gccagccagg
     6061 ggcgtccttc cattgattta ctgcgcgcgt taattgctca ggaaccgctg cttggcgcga
     6121 aagaaaaaca gcagaatatt gatgccacct ggcaggcgct ctcctccatg actcaggaac
     6181 aggcgaatac gctggtgatc aacgccgacg aaaatattct gcaaggctgg ctggatctgc
     6241 agcgcgtctg gtttgataac cgtaacgatc ccgacatgat gaaagccggg atcgccgact
     6301 ggcagaaacg ttatccgaac aatccgggcg cgaaaatgct gccaacgcag ttggttaacg
     6361 taaaagcgtt taaaccagcc tcgaccaaca aaatcgccct gctgttgcca ctgaatggcc
     6421 aggcagcggt atttggtcgc actattcagc aaggctttga agcggcgaaa aatatcggca
     6481 ctcagccagt ggcggctcag gtagctgccg cacctgccgc agacgtagca gaacaacctc
     6541 agccgcaaac tgcggatggc gttgccagcc cggcacaagc ctcggttagc gatctgaccg
     6601 gtgatcagcc tgcggcccag ccggtgcctg taagcgcccc ggcgacaagc accgcagcgg
     6661 taagcgcacc cgcaaatcca tccgcagagc tgaaaatcta cgatacctca tcacaaccac
     6721 ttagccagat cttaagccag gttcagcagg atggcgcgag tattgtggtt ggtccgttgc
     6781 tgaaaaataa cgttgaagag ttgctgaaga gcaacactcc gctgaacgta ctagcactga
     6841 accagccgga gaatatcgaa aatcgcgtca atatttgtta cttcgcgctt tcaccggaag
     6901 acgaagcgcg cgatgcagcg cgtcatattc gtgaccaggg taaacaagcg ccgctggtgc
     6961 tgatcccacg cagttcattg ggcgatcgcg tagccaatgc gtttgcgcaa gagtggcaga
     7021 aattgggcgg cggcaccgtt ctgcaacaaa aatttggttc caccagcgaa ttacgcgcgg
     7081 gtgttaacgg cggttctggt attgctttaa cgggtacccc gattactccc agagcgacaa
     7141 ccgactccgg catgacgacc aacaatccaa cgctgcaaac cacgccaacc gatgaccagt
     7201 tcaccaataa tggcggtcgt gtcgatgcgg tgtacattgt ggcaacgccg ggtgaaatcg
     7261 cttttattaa accgatgatc gccatgcgta acggtagcca gagcggtgca acgctgtacg
     7321 ccagctcccg cagtgcgcaa gggaccgctg gcccggattt ccgtctggag atggaaggct
     7381 tgcagtacag cgaaatcccg atgctggcgg gcggtaatct gccgttaatg cagcaggcac
     7441 tcagcgcggt gaataacgat tattcactgg ctcgcatgta tgcgatgggc gtcgatgcct
     7501 ggtcgctggc aaatcatttc tcacaaatgc gccaggttca gggttttgaa atcaacggta
     7561 ataccggaag cctgacggct aacccggatt gcgtgattaa caggaagtta tcatggctac
     7621 agtaccaaca aggtcaggta gtccccgcca gttaaccacc aaacagaccg gcgatgcgtg
     7681 ggaagtacaa gcgcgtcgct ggctggaagg caaaggactg cgatttgtcg ccgctaacgt
     7741 gaacgagcgt ggcggcgaga tcgatctgat aatgcgtgaa ggccggacca ccgtttttgt
     7801 cgaggtgcgt taccgccgtt ctgcgcttta tggcggcgcg gcagccagtg tgacccgcag
     7861 caaacaacac aaattattac agactgcccg cttgtggctc gcgcgtcata atgggagttt
     7921 tgatactgtg gattgccggt tcgatgtggt agccttcacc gggaatgagg ttgagtggat
     7981 taaggatacc tttaatgacc actcataatt aaggtttaag gattagcgtg caagaaagaa
     8041 ttaaagcttg cttcactgaa agcattcaaa ctcaaattgc ggcggcagag gcgcttccgg
     8101 atgccatctc ccgtgcggcc atgacgctgg ttcagtctct gctcaatggc aacaaaatcc
     8161 tctgttgtgg taatggaact tccgctgcca atgcacagca ttttgctgcc agcatgatca
     8221 accgtttcga aacggagcgg cccagcttac ctgccattgc actaaatacc gataatgttg
     8281 tcttaacggc gattgccaac gatcgcttac atgatgaagt gtatgcaaaa caggtgcggg
     8341 cgctggggca tgcgggagat gtattgttag ccatttccac ccgtggcaac agccgcgata
     8401 tcgtaaaagc agtagaagct gccgttacgc gtgatatgac cattgtggca ttgaccggtt
     8461 atgacggcgg tgaacttgca ggtttgttag ggccacagga tgtggagatc cgcatccctt
     8521 cgcatcgcag tgctcgcatt caggaaatgc atatgctgac ggtaaattgc ctgtgcgatc
     8581 tgatcgataa cacgcttttc cctcaccagg atgattaagg agaatacatg aaggcattat
     8641 cgccaatcgc agtccttatt tccgcgctgc tgttgcaagg ttgtgttgcc gctgccgtag
     8701 tgggtactgc tgctgtgggt accaaagccg caactgaccc acgcagtgtc ggcacccagg
     8761 tggacgatgg taccctggaa gtgcgcgtga acagcgcatt gtcgaaagac gaacagatta
     8821 agaaagaagc gcgcattaat gtaacggcct atcagggcaa agtgctgctg gttgggcagt
     8881 caccaaacgc tgaactttcg gctcgcgcca aacagattgc tatgggcgta gacggtgcca
     8941 acgaagtgta taacgagatt cgtcagggcc agccgattgg tctgggcgaa gcgtctaacg
     9001 atacgtggat caccaccaaa gtgcgttcgc agctcttaac cagcgacctg gtgaaatcgt
     9061 ccaacgtgaa agtgaccacc gaaaacggtg aagtgttcct gatggggctg gtgactgaac
     9121 gtgaagcgaa agcggcggca gatattgcca gccgggtgag cggcgtgaag cgggtaacta
     9181 cggcgtttac gtttattaaa tagcagcccc ttgtaatgcc tgatgcgacg cttgccgcgt
     9241 cttatcaggc ctacaacgat gcaaaccgta ggtcggataa ggcgtttacg ccgcatccga
     9301 catgttttcc ctcaaatcag aataacagcg ccagcccgcc gacaatcacg cctgacactg
     9361 ccaccatcgc ccccgtcagc cataaggctt tcgccgggaa cgctttgcgc agcataatca
     9421 gtgacggcaa actcaccgcc gggagcgtca tcaacaatgc cagcgccgga gcagttccca
     9481 tacctgccag catcatcgtt tgtacaatcg gaatttctgc tgccgtggga atgacaaaca
     9541 agcaccctgc taccgccatc gccaccaccc acatcaggct gttatcgaca gcaccatcgg
     9601 catgggggaa taaccagacg cgagcggcac ccaacaccag tactgcaagg atgtaaaccg
     9661 ggatcgtact ccagaaaagc gtccatagcg ccctgcccca gcgactaaaa aatccgccct
     9721 gtgcttccgg tatgtcaatt tcgaccggtg cttgcgtttg cggtgtttca cgcacccatt
     9781 tttgcaccag cgtcgcaatc agcaacacca tcaccagccc ggccaccaga cgaatcgccg
     9841 caaaacccca gccgaggaca aaccccataa acaccagcgt cgccgggttt aacaccggat
     9901 tgcccatcca gaatgccagc gcaccgccca tcgacacctg ttggcgacgc attcccgccg
     9961 cgaccggagc cgcacagcag gtacacatca tgcctggcag cgaaaacagc gttcccagca
    10021 gcgtgccgcg aaagcgcgat tgcccaagcg tacgcaacaa ccagtcacgc gggatcagca
    10081 cctgaatcaa cgaaccgagg atcaccccca gcaccgccgc tttccatacc gcgaggaaat
    10141 agatcatcgc gtaatccaac gccgcctgcc atggattagc atccgcctgc gcaaggatag
    10201 atttaccgat actgtgggtt tcggcagcag taaacgcttt gccgtagtaa ggttgccatt
    10261 tcacatacca gagaccggca atgacaacga gaaagaaaag cgcgggtttc caccactgaa
    10321 tgggcgttgc cgcctgagat gaagactgac cagtcatagc attccccagg aataagttat
    10381 gataattgag cgcgtgaata ttacgctcac tatcaattct tgggaataat tattcagctc
    10441 ttttgcgtaa ttctgaagag cttaaaatcg tcacaccttc atgctcgggc cgcatcgcct
    10501 ctgccagcat gacccgcgcc acgtctctcg catcaatgga tttccagtta ccgggtaaca
    10561 aacggaacag cggcgcaaaa agcgtttcgt tcatccgctg tttgctacga tcgcccagta
    10621 acatcgacgg gcgagcaatg gtcaatttcg gccagttctg ggcgattaat gcttcttcca
    10681 tctccccttt gacgcggtta tagaaaaacg gcgagtgggc attggcaccc atagcactga
    10741 ccaccagcat atgctgcgcg cccagtcgcc gcccggttaa tgcggtatcc actaccagcg
    10801 tgtaatcggc atgaataaac gcctctttgc tccccgcttc tcgccgcgtg gtgccgagac
    10861 aacaaaacac aatgtcgatg ggatcggtga cctgcgccag cgcgtcagtc agttgcggat
    10921 catggggatt aaataccccg ggcatatcgc ccaacggacg tcgcgtcggc gcggctatgg
    10981 cgttaacttt cggttcgtta atcaacatcc gcagcaggtg accgcccacc agccccgttg
    11041 cgcctgtaat cagtacctga ctcatcttcg ctcctttaca gaattgtctg ccttgcgctc
    11101 cacggctcat gcaccaggct taatagacca tgaggtaatt atccccgatt gtgggaaatt
    11161 cgcctcatcc aatgcaacaa cgcggaggaa gcatgagtaa gaaaattgcc gttttaatca
    11221 ctgatgaatt tgaggattca gaatttactt cacccgcaga cgagttccgt aaagccggac
    11281 acgaagtgat taccattgaa aaacaagcgg gtaaaacggt gaaaggcaaa aaaggagaag
    11341 ccagcgtgac catcgataaa tccatcgatg aagtgacgcc agcggagttt gatgccctgt
    11401 tgctaccggg cggtcattca ccggattatc tgcgtggcga caaccgtttt gtcaccttta
    11461 cccgtgattt tgtgaatagc ggcaaacctg ttttcgccat ctgtcacggt ccgcagctgc
    11521 tgatcagcgc cgatgtgatt cgggggcgca aactgaccgc ggttaaaccg atcattattg
    11581 atgttaaaaa cgcgggcgcg gaattttacg atcaggaagt cgtggtcgat aaagatcagc
    11641 tggttaccag ccggacaccg gacgatctgc cagcgtttaa ccgcgaggcg ttacgcctgc
    11701 tcggtgcctg agccgcgtag ccaaatcatt tttttaccaa agcccagcgt gttgtcggtg
    11761 aatttgattt catcgggccg gatttcccac accggggccg acagcattct ggcaaccgga
    11821 aagcgacgat tgtacgcctt gcgcgcgagg tcgctttcct caccttccag cctgcggatc
    11881 tcacctttaa actgcacgcc gcgaattaac gctaccgttt tcggctgacc gtttaccgtt
    11941 ccggcaaccg cagcctgcgg cccacgcatc tgcgcgtggc gcgttttttc ttccgtcaaa
    12001 atgtagaagg cgactttctg cacatcaaac agataaaagg cattggcgca ccagagttcc
    12061 ccttcctgct gcacacacca ggtgacaaca tgttgttttg ccagccaacg gctgatggcg
    12121 gtgagtgttt ccattgctgt tctcttttat actgtgggcc tgaccttaac atgcgacgca
    12181 gacgatgaca ccctggtttc tttacttgat ccgtaccgcc gacaataagc tttataccgg
    12241 gatcaccacg gatgtcgaac gccgctatca gcagcaccaa agcggcaaag gggcgaaagc
    12301 actgcgcggg aaaggagaac tgacgctggc gttttctgcg ccagtgggcg atcgttcgct
    12361 ggcgttacgg gcggaatatc gcgttaagca actgaccaaa cggcaaaaag agcgtctggt
    12421 agcggaaggc gcagggtttg caaagctgtt aagcagtctg caaaccccgg agattaaaag
    12481 cgattgaaat gctcgtgata ctcaaccaga ccagttacgc cattcaacgc gtcatccgct
    12541 aaacgatgta cctggaaggc gctttcagtg cccggccagc ggcaacgcag atcgtgatgc
    12601 gccgccagtt caaagccgaa acggctgtat agcgccggat cgcccagcgt caccactgcg
    12661 gcatagccga actcattaag cgaatcgagt ccttcataga ccagttggcg tgccagcccc
    12721 tgtccacggt atttttcatc taccgccagc ggtgccatgc cgacccattg caggtcttcg
    12781 ccctgcacat caaccggact aaatgccaca tagccaatga cctgaccttc gtcatctgtc
    12841 gccaccagcc ccagcgtcag aaagccatct tcacgcagat cgtgaaccag cttcgcttcc
    12901 gcatcacttt cgaatgaacg acgcagcagg gcatcaatac ccggcgcatc aatgggaatt
    12961 tctactcgaa ttagcatggt tcacctaccg atgtctgttt ggtttcaggc gcggttttca
    13021 ttcccgcctc aacaaaatcc gccagttgca gcagcatcat gcgtaaggct ttcggcattt
    13081 gctccagttc aatggcgtcc atcaggtttt tcacatacag ccccagctcc gtatcgcctt
    13141 caatcaccag ccgacgctgg aagaagagcg tatccggatc ttgtttacgc gccgcaatca
    13201 tcagcagatc gctggcgtcg gcactaaaac tcacatcagc ttgcgcgttc tggctgacca
    13261 ccagtttgcc attcaccacc gaggtaaacc attgcaggtc aatatcacgc acatgaatac
    13321 ttaaccagcg gccttcaaga aactccagct caccatcatc cagcgcctgg cggaattgcc
    13381 agcttaagac ctgctcaaga acctggcgtt ttagcgcaaa tggcgtcagt tttaccggta
    13441 cactcaacag agatggcccc aaatgcacaa tacgggaacg cagtttatcc aacacgagtt
    13501 ttactccctg tttcaacaat catcctattt tgccatatca gaaaaataac atagcggtat
    13561 aaatcaacaa ttccatatga aattgctgct accaccaata caactttaac tgccttaaat
    13621 caaaaattgt cgcagcaagg ttaactaaaa tcccagttcg ttaacatttt tgcgttttga
    13681 tagcgcaacc ttcaggaaaa attatggagc tgctctgccc tgccggaaat ctcccggcgc
    13741 ttaaggcggc catcgaaaac ggcgcagatg ctgtttatat cgggctaaaa gatgatacca
    13801 atgcccgtca cttcgccggc cttaacttta ccgagaaaaa attgcaggaa gcggtgagtt
    13861 ttgtccatca acatcgccgc aaacttcaca tcgcgattaa cacttttgcg catccggacg
    13921 gttacgcccg ttggcagcgt gccgtggata tggcggcgca gctgggtgcc gacgcgctga
    13981 tcctcgccga cctcgccatg ctagagtacg ccgccgagcg ttacccgcat attgagcgtc
    14041 atgtgtcagt gcaggcttcg gcgaccaatg aagaggcgat taacttttat catcgccatt
    14101 ttgacgttgc tcgcgtggtg ctgccgcgcg tgttgtcgat tcatcaggtg aaacaactgg
    14161 cacgggtcac acctgtcccg ctggaagtct ttgctttcgg cagcctgtgc attatgtcgg
    14221 aaggtcgttg ctatctgtcg tcgtatctga cgggtgagtc gcccaacacc gtgggcgcgt
    14281 gttctccggc ccgtttcgtg cgctggcagc aaacgccgca ggggctggaa tcccgcctga
    14341 acgaagtgct gatcgaccgt tatcaggacg gcgaaaacgc aggttatccg acgctatgta
    14401 aagggcgtta tctggtggac ggcgagcgct atcacgcgct ggaagaacca accagtctca
    14461 ataccctgga actgctgccg gagttaatgg cggcgaatat tgcttcggtg aaaattgaag
    14521 gccgccaacg tagcccggcg tatgtcagcc aggtggcgaa agtctggcgt caggctatcg
    14581 accgttgtaa ggccgatccg caaaacttcg taccgcaaag cgcgtggatg gagacgctcg
    14641 ggtcgatgtc cgaaggcacg cagaccaccc ttggcgcgta tcaccgtaaa tggcagtgag
    14701 aagagcaatg aaatattcct tagggccagt gctgtggtac tggccaaaag agacgctgga
    14761 agatttttat cagcaggccg ccaccagcag cgccgacgtg atttatcttg gcgaagcggt
    14821 ttgcagcaag cgccgggcaa ccaaagttgg cgactggctg gagatggcaa aatcgctcgc
    14881 cggaagtggt aagcagattg tgctctccac gctggcgctg gtgcaggcat catctgaact
    14941 gggcgaactg aaacgctatg ttgagaacgg tgagtttctg attgaagcca gcgatctcgg
    15001 cgtggtgaat atgtgcgctg aacgcaaact gccgttcgtc gccggacacg cgctgaactg
    15061 ctataacgcg gtgacgctga aaatattgct caaacagggc atgatgcgct ggtgtatgcc
    15121 ggtggagctt tcccgcgact ggctggtgaa tctgcttaat cagtgcgatg agctgggcat
    15181 tcgcaaccag tttgaagttg aagtcctgag ctacggtcat ctaccgctgg cctactccgc
    15241 tcgctgcttt accgcgcgtt cggaagaccg cccgaaagat gagtgtgaaa cctgctgcat
    15301 taagtatccg aacgggcgca acgtgctgtc gcaggaaaac caacaagtgt ttgtactcaa
    15361 tggcattcag accatgagcg gctacgttta caacctcggt aacgagctgg catccatgca
    15421 gggcctggtt gatgtggtcc gcctgtcacc gcagggtact gacactttcg cgatgctcga
    15481 cgccttccgc gctaatgaaa atggcgctgc accactgccg ttgacggcga acagcgactg
    15541 taacggctac tggcggcggc tggcaggact ggagctgcaa gcctaagtaa atagctcact
    15601 ttgttaacaa ctttaactac tctttaatgc agtattaaag attaatcggt aacaaagtga
    15661 gctgttatga ctgataaaac cattgcgttt tcgctactcg atctggcccc cattcccgaa
    15721 ggttcttcag cgcgagatgc attctcccac tctctcgatc tcgcccgtct ggctgaaaag
    15781 cgcggctatc atcgctactg gctggcagaa caccacaata tgactggcat tgccagtgct
    15841 gccacgtcgg tattgattgg ctatctggcg gcgaatacca ccacgctgca tctggggtct
    15901 ggcggcgtga tgttgcctaa ccactcaccg ttggtcattg cagaacagtt cggcacgctc
    15961 aatacactct atccggggcg aatcgatttg gggctgggtc gtgctccggg tagtgaccag
    16021 cgaaccatga tggcgctacg tcgtcatatg agcggggata ttgataattt cccccgcgat
    16081 gtcgcggagc tggtggcctg gtttgacgcc cgcgatccca atccgcatgt gcgcccggta
    16141 ccaggctacg gcgagcaaat ccccgtgtgg ttgttaggct ccagccttta cagcgcgcaa
    16201 ctggcggcgc agcttggtct gccgtttgcg tttgcctcac acttcgcgcc ggatatgttg
    16261 ttccaggcgc tgcatcttta tcgcagcaac ttcaaaccgt cggcacgact ggaaaaacca
    16321 tacgcgatgg tgtgcatcaa tattatcgct gccgacagca accgcgacgc tgaatttctg
    16381 tttacctcaa tgcagcaagc ctttgtgaag ctgcgccgtg gcgaaaccgg gcaactgccg
    16441 ccgccgattc aaaatatgga tcagttctgg tcaccgtctg agcagtatgg cgtgcaacag
    16501 gcgctgagta tgtcgctggt gggcgataaa gcgaaagtgc gtcatggttt gcagtcgatc
    16561 ctgcgcgaaa ccgacgccga tgagattatg gttaacgggc agattttcga ccaccaggcg
    16621 cgcctgcatt cgtttgagct ggcgatggat gttaaggaag agttgttggg atagtgtgtc
    16681 ttaacgcggg aagccttatc cgagctggca acgctgtccc acgtaggcct gataagcgaa
    16741 gcgcatcagg cattgtgtag gcagcagaaa tgtcggataa ggcccgctga ttactgatac
    16801 accggcagca aattaaagct cgataagata tggaccagtg cgttgccaac gccaaatacc
    16861 agaatcagcg taatcatcgg cttgccgccc cagacgcgga atttcgggct gccaaagcgt
    16921 ttacgcgatg cgcgggccag cagtgccgga acaattgccg cccagatggt agctgctaaa
    16981 ccagcataac caatggcgta caggaatccg ttcgggaaca gcagaccacc cacaacagga
    17041 ggggcaaagg tcagcaatgc cgttttcaag cggcccacag ccgagtcgtc gaaaccaaac
    17101 agatctgcca gatagtcaaa caaacccagc gttacgccga ggaacgaact cgctaccgca
    17161 aagtttgaga acacgaccag cagcagatcc agactacggc tgttcagtac gccgcttaac
    17221 gcctgtacca gcacatcaat attaccgccc ttctctgcaa tatcgataaa ctccggacgc
    17281 gggatgttac ccatcgtcgc cagcaaccag atggtataca gcgccagcgc catcagcgta
    17341 ccgtacacca gacatttcac gatggttttc ggatctttgc cgtaatactt catcaggctt
    17401 ggcacgttac cgtgataacc aaacgatgcc agacagaacg gcaaggtcat caacagatac
    17461 ggtgcataag acgcattgct ttcagcgacg ttgaacaatg tcgcaggctg cacatgcccc
    17521 agcaggctac cgaaggtgag gaagaaggta atgactttcg cccccaatac aatcgctgtc
    17581 atgcgactga cggctttagt gctcaaccac accacaaacg ctaccagcaa tgcaaaacca
    17641 aaacccgccg cccgtgccgg gacgtttagt gacatctctg cgaaggtgtg atgcagaatc
    17701 gaaccactgg cagaaatata ggcataggtc aggatataga gcacaaaggc aatggaaatg
    17761 ccgttgacca cgttccagcc tttgcccagc aaatctttgg tgatggtgtc aaaactcgaa
    17821 ccgattctgt aattcaggtt agcttccaga atcatcaagc cggaatgcag catacagaac
    17881 caggtaaaga tcagcgccgc cattgaccag aaaaaccacg ccccggacat gaccactggc
    17941 agagaaaaca tccctgcgcc aataatggtg ccgccgataa tcaccacgcc gccaagcagc
    18001 gacggtgacg tttgggtggt ggttagtgtt gccatgaggg cttctctcca gtgaaaaata
    18061 gtgcgactgc gttgttatgt attgcactgt accagtacgc gagtacaaaa gacagaaaaa
    18121 aagccccgat ggttcaaatc ggggctgtat atattatttt acagactgtg ttcgctgttc
    18181 agcgatgatt acgcatcacc accgaaacga cgacgaccgg tagagtcatc acgacgcgga
    18241 gcgcggcctt cacgacgttc gccgctaaaa cgacgaccat caccacgacc accttcacgg
    18301 cgttcaccgc tgaagttacg accaccttca cgacgctcgc cactgaaacc acgaccaccg
    18361 ccacgacgct ctccgccagt atgcggctgt gcatcgccca gtaattgcat attcatcggc
    18421 ttgttcagaa tgcgagtacg tgtaaagtgt tgcagcactt cacccggcat acctttcggc
    18481 agttcgatgg tggagtgaga agcaaacagc ttaatgttac cgatgtaacg gctgctgatg
    18541 tcgccttcgt tagcaatcgc accaacgata tgacgaactt caacaccatc atcgcggccc
    18601 acttcaatac ggtacaactg catgtcacca acatcacgac gttcacgacg cggacgatct
    18661 tcacggtcgc cacgcggacc acggtcgtta cgatcgcgcg gaccacggtc atcacggtca
    18721 cggaattcac gtttcggacg catcggcgca tctggcggaa caatcagagt acgttcaccc
    18781 tgtgccattt tcagcagtgc ggcggccaga gtttcgagat ccagctcttc accttctgca
    18841 gtcggctgaa ttttgctcag cagtgcgcgg tattgatcca gatcgctgct ttccagctgc
    18901 tgctgtactt tagcggcgaa tttttccaga cggcgtttgc ccagcagttc tgcgttcggc
    18961 agttctactt ccggaatagt cagcttcata gtacgttcaa tgttgcgcag cagacgacgc
    19021 tcgcggttct caacgaacag cagcgcgcgg ccagcacgac ccgcacgacc ggtacgaccg
    19081 atacggtgaa cgtaagactc agaatccatc gggatatcgt agttaaccac caggctgata
    19141 cgctcaacgt ccaggccacg ggctgcaacg tcggtcgcaa tcaggatgtc cagacgacca
    19201 tctttcaggc gttccagtgt ctgctcacgc agcgcctggt tcatgtcacc gttcagcgcg
    19261 gcgctgttgt agccgttacg ctcaagagct tcagccactt ccagagtcgc atttttggta
    19321 cgaacgaaga taatcgccgc atcaaaatct tccgcttcca ggaaacgtac cagtgcttcg
    19381 tttttacgca taccccagac agtccaatag ctctggctga tgtcaggacg ggtagtcacg
    19441 ctggactgaa tgcgcacttc ctgcggctct ttcataaagc ggcgggtaat gcgacgaatc
    19501 gcttccggca tggttgcaga gaacagagcg gtctgatgac cttccgggat ctgcgccata
    19561 atagtttcaa cgtcttcgat gaagcccatg cgcagcattt cgtcagcttc atccagaacc
    19621 agaccgctca gtttagagag gtccagagtg ccacgtttca ggtggtccag cagacggccc
    19681 ggagtaccga caacgatctg cggcccctga cgcagggcgc gtaattgcac gtcataacgc
    19741 tggccgccgt acagggcaac cacatttacg ccgcgcatgt gtttagagaa atccgtcatt
    19801 gcttcagcaa cctgtaccgc cagttcgcgg gtcggtgcta gcaccagaat ctgtggtgct
    19861 ttcagctcag gatcaagatt ttgcagcaga ggtaaagaga atgctgcagt ttttccgctc
    19921 cccgtctggg ccatacccag tacgtcgcgg ccattcagca gatgtggaat acactccgcc
    19981 tgaattggag atggtttttc gtaacccaga tcgttaaggg cttcaaggat aggagccttc
    20041 aggcccagat ctgcaaaagt ggtttcgaat tcagccatgt agtacgtgtg cctcaaaatt
    20101 aatggcggcc agtctacata actcatcatg aaattgatca gcaattttca ttgaaaagtg
    20161 tgaaccggct caaagtaggt gtattaacga acaacaacgc cctcacccgt taaggtgatg
    20221 gcaatcaaaa aagattacgg gctgatgtgt acgtcagcta ttgctggtcc gattctgcca
    20281 ggtcatcttg gtcctggccc aggagcgata attccaacaa tgcgtatcgg tgctcaacaa
    20341 agttatgaac gttgttggca accgccagtt tgaacagtgc cgtggcgctg tccaaatccc
    20401 ccagacttag gtagtactta cctaaataga agttggtttc actgagatgc tcagcgagcg
    20461 aggtgttatc cgttgcgtcc gccttgagcc tttccattaa cgtttgttcg ctaatgttgc
    20521 ccaggtagaa ctcgacaatg ttccatcccc actgttcctt atccgatttt tcgaagtgct
    20581 gtttcaacac ttctttagcc tgcttctcat cgagcttctg ctcggcgaga taaagccaca
    20641 gactacggaa aggatcattg ggatcgtctt gataaaacgc cagcagatca tcttgcgcta
    20701 acttgtcacg accgccgtaa tataatgcga tcccgcgatt caagtgcgcg tagttgtaag
    20761 ttggatcaag ctcaagtaca gaatcaaacg cttcataggc agcatcaaaa ttgcctgcct
    20821 gcgttaaata tatgcctaag taattgaata cttcaggcat atccggtcgg attgccagcg
    20881 cttgcgaaaa atcgttacgc gctaatgccc tcagaccgag actatcatac aacactccgc
    20941 gctcatataa aagctgtgcg cgttcgtcat cggttaaagc ccgactggca aggatttgtt
    21001 ccatacgtgc cagaatcact tcctgctgta aagtcggttg caatggtacc gcgaggactt
    21061 cacttttacg ccaggaagta ttactgcatc ctgcaagcgt aagtgctgtc gcaacgaaac
    21121 accagcgcaa aaaaggcttc atttcccact cccgaagacc acggttgaat gaacgtcctg
    21181 ttcccggttg ctaacaaggc gtcctgcccg gttaaaagcc ccccgccgca gcggagggca
    21241 aatggcaacc ttactcgccc tgttcagcag ccggagcttc cggtgctgca gcaggttgag
    21301 actgctcagt cgcttcttta atgctcagac ggatacggcc ctggcgatca acttccagaa
    21361 ctttcaccgg tacttcctga cccatctgca ggtaatcggt cactttctca acgcgtttgt
    21421 cagcgatttg agagatgtgg accagacctt ctttaccgcc gccgatggca acaaatgcgc
    21481 cgaagtcaac gatacgggtc actttaccag tgtagacgcg gcccacttca atttctgcag
    21541 tgatctcttc gatacgacga atagcgtgtt tcgctttctc gccatcggtc gctgcgatct
    21601 tcacagtacc gtcatcttcg atttcgatgg tggtgccagt ttcttcggtc agggcacgga
    21661 tcacagagcc gcctttaccg ataacgtctt tgatcttgtc cgggttgatc ttgatggtat
    21721 ggatacgcgg agcgaactca gagatatcgc cacgtggcgc gttgatcgcc tgttccatta
    21781 cacccaggat gtgcagacgc gcacctttag cctggttcag tgcaacctgc atgatctctt
    21841 tggtgatacc ttcaattttg atatccatct gcagtgcaga gataccgtcg cgggaacccg
    21901 caactttgaa gtccatatcg cccaggtgat cttcgtcgcc caaaatgtca gacagtacaa
    21961 cgtagttgtc gccttctttc accagaccca ttgcgatacc cgcaacggca gctttgattg
    22021 gcacacctgc gtccatcagc gccagagacg caccgcacac ggaagccata gaagaagaac
    22081 cgttggattc agtgatttca gacacaacac gtacggtgta cgggaatttg tccatatccg
    22141 gcattactgc cagcacgccg cgcttcgcca gacgaccgtg accaatttca cgacgcttcg
    22201 gagaaccgac catgccggtt tcgcctacgg agtacggagg gaagttgtag tggaacagga
    22261 aggtatcggt acgttcgccc atcagttcat caagaacctg cgcgtcacga gcagtaccca
    22321 gcgttgcggt aaccagcgcc tgcgtttcac cacgggtgaa cagcgcagaa ccgtgagtac
    22381 gcggcagcac gccagtacgc acatccagac cacggatcat atctttttca cgaccatcga
    22441 tacgcggttc gcctgccagt acgcggctac gaacaacgtt tttctcgatc gcgtgcagaa
    22501 tttcacccag ttcgttttcg tccagggttt cgtcttcagc aagcagcgtc gcgatggttt
    22561 cagatttgat gacatcaacc tgcgcataac gctcttgttt gtcggtgatg cggtaagcat
    22621 cgctcagacg agcttcagcc agtgcagcaa cgcgcgcgtt cagcgcttcg tttaccggct
    22681 ccggctgcca gtcccaacgc ggtttaccgg cttctttcac cagttcattg atgttctgaa
    22741 taacaacctg ctgttgttca tgaccgaaca ctactgcgcc cagcatctgg tcttcgctca
    22801 gcagttcagc ttcagattca accatcagta cagcggcttc agtacccgca acaaccagat
    22861 ccagtttgct ctctttcagc tcgtcctgag tcgggttcag tacgtactgg tcattgatgt
    22921 aacctacgcg ggcagcacca atcgggccat tgaacggaat accagacaga gacagtgctg
    22981 cggaagcacc aatcatcgcg acgatatccg ggttaacctg cgggttaaca gaaaccacgg
    23041 tggcgataac ctgaacttcg ttgacgaagc cttccgggaa cagcgggcga atcgggcggt
    23101 caatcagacg cgcgatcagg gtttcgcctt cgcttggacg accttcacga cggaagaagc
    23161 tacccgggat acgaccagca gcgtaggtac gctcctgata gttaacggtc agtgggaaga
    23221 agtcctgacc tggtttggct tttttctggc caacaacggt aacaaatacc gcggtgtcat
    23281 ccatgctaac cataacagcg gcagtagcct gacgagccat catgccggtt tccagagtca
    23341 cggtgtgttg gccgtactgg aatttacgaa cgatcggatt aagcaatgta atatcctttc
    23401 tctttcttag acagtacctt acggcactgg tgttaatacc cgatcttctg cgcatcctcg
    23461 cgactaatga caaccctaac ccagctctat gtgggtaaag cctctcatta gccgcgcgaa
    23521 cctctgcaac ggaagatcat tcatagcaac aatacattag tttccagtga attgctgccg
    23581 tcagcttgaa aaaaggggcc actcaggccc ccttttctga aactcgcaag aattagcgac
    23641 gcagacccag gcgctcgatg agccgggtgt aacgtgctac gtctttacgt ttcaggtagt
    23701 cgagcagttt acgacgctga gaaaccatgc gcagcagacc acgacggctg tggtgatctt
    23761 ttttgtgctc tgcaaagtgg ccctgcaggt ggttgatctg tgcagtcagc agtgctacct
    23821 gaacttcggt agaaccggtg tcgtttgcgt cacgaccaaa ctcagaaacg attttagctg
    23881 ttgcttcagt acttagagac attttaaaac tccaaagtat atagaatgaa aagacgccga
    23941 tctctaattc agcgatccca gtgtacgtta cgcaaagtgt taaacaattt acgcgacgtt
    24001 aagcggcagt attctactcg tagcgacctg ttatcgcaag acggttaaca ttacgccggg
    24061 tattcaacca ccagacgacg aggcgcaacg cggccttcat cgtcaatttc gcccataccg
    24121 ataaacttgc cgttctcacc ttccgtaacg cgaaccagtc cttccagcgg cgcgccagat
    24181 gtacgaaccg ggttaccatt tttgaagtaa acagaagacg ttaacggaag attcaccacc
    24241 gggtagtccg aagctggact gtccattggc atcagtaatg gatcaagcaa ctccgcagcc
    24301 ggaatatcct gctgttcggc ttgttcgacc agttcacgca ggtgctccag ggtcaccatc
    24361 cgttcaaccg gatatttact taccgccaga cgacgcaggt aaatcacatg cgcgccacag
    24421 ccgagttttt cacccaggtc atcaatgatg gtgcggatat aagtgccttt tgagcagtga
    24481 atttccagct ccagctcatt gccttcatgg cgaataaaca gcaattcata aacggtaatt
    24541 ggacgcgctt cacgcggaac ttcaatgccc tgacgcgcat attcgtacag ttttttgccc
    24601 tggtatttca gtgctgaata catcgatggg atctgttcga tatcgccacg gaaagtatcc
    24661 agtgccgccg ccagttgttc tgcactaaag gttaccggac gctcttcaac gatttgtccg
    24721 tcgacgtcag aagtatcggt acgctgtcca agacgcgcaa tgacccgata gcgtttgtcg
    24781 gagtccagca gatactggga aaacttcgtc gcttccccga ggcaaatcgg caacatgccg
    24841 gtcgccagcg ggtccagcgc accggtatgc ccggcacgat tggcgttata tatacgtttc
    24901 actttttgca gcgcatcgtt gctggacata ccctgcggtt tatccagcaa caaaacgccg
    24961 ttaatgtcgc gaccgcgacg acgaggacga ctcattagtc ctccttgctg tcgtccgggt
    25021 taacacgacg ttcttcgtca tgtttgacca cgctggtcac caggtttgac atacgcatcc
    25081 cttcaaccag agagttgtcg tagaagaagg tcagttccgg cacgatacgc aggcgcatcg
    25141 ctttacccag caggctgcgg atgaaaccag aagcttcctg caacgctttg atgcccgctt
    25201 taaccgcgtc ttcatctttg tcgttgagga aagtcacata cactttggca tacgccaggt
    25261 cgcgagacat ttcgacaccg gaaacggtgg tcatcatgcc cagacgagga tctttaattt
    25321 cacgctgcag gatgagagcg atctcttttt gcatttcctg cgctacgcgc tgcgggcgac
    25381 caaattcttt cgccataata aattctcctg acaaaaaagg ggctgttagc ccctttttaa
    25441 aaattaattt caggtggaag ggctgttcac gttggcctga taagacgcgc cagcgtcaca
    25501 tcaggcaatc catgccggat gcggcgtaaa cgccttatcc cgcatggaac cctaaaaacc
    25561 ttaagcgatg gtacgttgga tctcgatgat ttcgaatact tcgatcacat cgccagtgcg
    25621 gacgtcgttg tagttcttaa cgccgatacc acattccata ccgttacgga cttcgttaac
    25681 gtcatctttg aagcggcgca gggactccag ctcgccttca tagataacca cgttgtcacg
    25741 cagaacgcgg atcgggttgt gacgtttaac cacaccttcg gtaaccatac agcctgcgat
    25801 ggcaccaaat ttcggcgatt tgaacacgtc acgaacttcc gccagaccga taatctgctg
    25861 tttcagttcc ggagacagca taccgctcat cgccgctttc acttcgtcga tcaggttata
    25921 gatgacggag tagtaacgca gatccaggct ttccgcttca atcactttac gtgcagaggc
    25981 atcagcacgt acgttgaagc caaccaggat ggcgttggac gccgcagcca aggtggcgtc
    26041 ggtttcggtg ataccaccta cgccagaacc gatgatcttc actttaactt cgtcagtaga
    26101 cagtttcagc aaggagtcgg agatcgcttc gacagaaccc tgtacgtctg ccttcaggac
    26161 gatattcact tcgtgaactt cgccttcggt catgttggcg aacatgttct cgagtttaga
    26221 tttctgctga cgcgccagtt taacttcgcg gaatttaccc tgacgataga gtgcaacttc
    26281 acgcgctttc ttctcgtcac gtacaacggt aacttcatca cccgcagccg gaacaccgga
    26341 caagccgagg atttccaccg gaatggacgg acccgcttcc agcacttcct gacccagttc
    26401 gttacgcatc gcacgaacac gaccgtattc gaagccacac agaacgatat cgcccttgtg
    26461 cagagtacct tcacgtacca gaacggtagc aaccggacca cgacctttat cgaggaagga
    26521 ttcgataacc gcaccgctcg ccataccttt acgtaccgct ttcagctcca gaacttccgc
    26581 ctgcagcagg atagcgtcca gcagttcatc gataccggta cccgctttcg cagatacgtg
    26641 tacgaactgg ctttcaccgc cccactcttc cggcaggatg ccgtactggg agagttcgtt
    26701 cttaacgcga tccggatcgg cttctggttt atcgatcttg ttcactgcaa ccaccaccgg
    26761 tacctgcgcc gctttcgcgt gctggattgc ttcgatggtc tgcggcatta cgccgtcgtc
    26821 ggcagcaaca accaggacta cgatgtcagt tgcctgcgca ccacgtgcac gcattgaagt
    26881 aaacgcggcg tgccccgggg tgtccaggaa ggtgatcatg ccgttttcag tttcaacgtg
    26941 gtatgcacca atgtgctggg taatgccgcc cgcttcgcca gaggccactt tcgttgaacg
    27001 aatgtagtcc agcagagagg ttttaccgtg gtcaacgtga cccatgatgg tcacaaccgg
    27061 agcgcgcggt tcagccgcag ctcccgtgtc acggtcgctc attaccgcct cttccagctc
    27121 gttttcacga cgcaggataa ctttgtggcc catctcttca gcaaccagct gtgcggtttc
    27181 ctgatcgata acctggttga tggttgccat tgcgcccagt ttcatcatcg ctttgatgac
    27241 ctgagagcct ttaaccgcca tcttgttcgc cagttcgcca acggtgatag tttcgccgat
    27301 cacaacgtca cggttaacgg cctgagcagg cttctggaag ccttgctgca gcgaagaacc
    27361 tttacgtttt ccgcccttac cgccacgtac tgctgcgcgt gcttcttcac gatcagcttt
    27421 tgattcagcg tgtttgttgc ctttcttcgg acgcgctgct ttcgcgttac gaccacggcc
    27481 acggccgcct tcgacttcac gatcgctttc gtcttctgcc tggcgagcat gttgagaagt
    27541 agtgacgtga taatcgctgg aatcctcagt cggttccgcg ttatcagtcc atttgttttc
    27601 ttccgccata cgacgtgctt cttcagcaac gcgacgtgct tcttcttcga gtttacgacg
    27661 cgcttcttct tcggctttac gcttgagctc tgcagcttcc tgctcacggc gggctttttc
    27721 agcctgggcg tttttagtca tatcgtcttg ttgattgctc actttgtctt tttccgcagc
    27781 ttcacgtttc gcttgttcag cagcttcacg cttagcttgt tctgcggcct cacgttcagc
    27841 tttttgttgc gcctcgcgtt tagccgattc ttctgcctca cgacgggctt gctcttccgc
    27901 ttcacgctgc gcttgctctt ccgctgcaag gcgttcagcc tcttgcggat cgcgtttcac
    27961 aaaggtgcgt ttcttgcgga cttcgatttg taccgatttg ctttttccac cggtaccagg
    28021 aatgttaagg gtgctgcgtg ttttacgttg cagcgtcaat ttgtccgggc ctgaattttt
    28081 ctgattcagg tggtcaatca aagtctgttt ctcttgtgca gacacagagt cgtcagcaga
    28141 cttccggata cctgcatcag caaattgctg taccaggcgt tccacggagg tctgtcgctc
    28201 tgcggccagc gttttaatcg ttacatctgt catgctgttc cttcctgcta cagtttatta
    28261 cgcttcgtca ccgaaccagc aaatattacg ggcagccata atcagtgctc cggctttttc
    28321 gtcggtcaac ccttcgatat cagccagatc atcaatgccc tgttcggcga gatcttccag
    28381 cgtacaaacg ccacgggcgg ccagtttgaa tgccaaatca cgatctaccc cttcaaggtt
    28441 cagcagatcg tcagccggtt tgttatcacc gaggctttct tcctggacct gtgcaatggt
    28501 ggccagtgca tttttagcac gctcgcgcag tgcttcaacg gtcggctcat caaggccttc
    28561 gatttccaac agctctttca tcggcacata ggccagttct tccagcgtcg agaagccttc
    28621 ctctaccaga acagtcgcga agtcttcgtc gatgtcgaga tatttggtga aggtgtcgat
    28681 cgctgcgtgc gcttccgcct gatgcttagc ctgcaggtcg tcaacggtca tcacgttgag
    28741 ttcccaaccg ctcagctgcg aagccagacg cacgttctga ccgttacggc caatcgcctg
    28801 cgccaggtta ccggcttcaa cggcgatatc catggtgtgt ttatcttcat ccaccacgat
    28861 agaagcaacg tctgccggtg ccattgcgtt aatcacaaac tgcgccgggt tatcatccca
    28921 caggacgata tcgatacgct cgccgcccag ttcagtagaa accgcctgaa cacgcgcgcc
    28981 acgcatacct acgcaagcac ctaccggatc gatacgttta tcgttggttt tcaccgcgat
    29041 tttcgcacga gaacccgggt cgcgagccgc tgctttaatt tcaatcactt cttcgccgat
    29101 ttctggcact tcaatacgga acagttcgat cagcatttcc ggcttggaac gagtgacgaa
    29161 cagttgcgcg ccacgcgctt ccgggcgaac ggaataaagc acgccacgaa cgcggtcgcc
    29221 agggcggaag ttttcacgcg gcagcatatc ttcgcgcagg atcacggctt cagcgttgtt
    29281 gcccagatcc agagagatgt tgtcgcggtt tacttttttc accacgccgg tgatgatttc
    29341 accttcgtgt tcacggaact gatcaaccac catcgcacgt tcggcttcac gcactttctg
    29401 cacgataacc tgttttgccg tctgggtggt gatacggtca aaggtaacag actcaatctg
    29461 atcttcaacg taatcgccca ggttcaggct ttcatcttca taacgtgcgg cttcaagggt
    29521 gatttccttg gtcggctggg tgacttcatc aacaactaac cagcgacgga aggtgtcaaa
    29581 atcaccgctt ttgcgatcga tctgtacgcg gacgtcgatc tcttgttcat attttttctt
    29641 tgttgctgtc gccagcgcgc tttccaatgc ttcgaaaatc ttctcgcgag gtagcgcctt
    29701 ttcattggat acggcttcaa ctacagccaa aatttctttg ttcatcgcgg gcttttcacc
    29761 tcatccagac tattaaaagt ggggaaccaa gttcgccttc tggatattac tcagcgcgaa
    29821 cacttcatct ttaccttcga cggtaactgt gatcatttca ccgtctaccg ctttgataac
    29881 gccctgccat ttacgacggt tttgtaccgc catacggaga accagagtca cctcttctcc
    29941 gacaaaacgg gcgtagtgtt cagccgtgaa cagtgggcga tcgagacccg gtgaggagac
    30001 ttccaggtta taagcaacgg tgatgggatc ttcaacatcc agcacagcac ttacctggtg
    30061 gctcacatca gcacaatcat caacattgat gccatcttca ctatcaatat agatgcgcag
    30121 tgtggatgtg cgaccgcgaa taaattcgat gccaaccagt tcaaaaccca gggcctcaac
    30181 tggcgcagta atcatctctg ttaatttttg ctctaatgtg gacaagccca cccccaagac
    30241 ataaaaaaag ggcctaaagc ccagttattc tgtagtcaga taacaaaaaa ccccgataaa
    30301 tcggggcttt atataactga accctataac cgcaactgcg gtctggagca ctttccagaa
    30361 ggattttttc aaatcccact acgaaggccg aagtcttcac agtatatttg aaaaaggact
    30421 ctaagggaaa gtggttgcgg gggccggatt tgaaccgacg accttcgggt tatgagcccg
    30481 acgagctacc aggctgctcc accccgcgcc tgaaacgtgg caaattctac tcgttttggg
    30541 taaaaaatgc aaatactgct gggatttggt gtaccgagac gggacgtaaa atctgcaggc
    30601 attatagtga tccacgccac attttgtcaa cgtttattgc taatcacgtg aatgaatatc
    30661 cagttcactt tcatttgttg aatacttttg ccttctcctg ctctccctta agcgcattat
    30721 tttacaaaaa acacactaaa ctcttcctgt ctccgataaa agatgattaa atgaaaactc
    30781 atttattttg cataaaaatt cagtgagagc ggaaatccag gctcatcatc agttaattaa
    30841 gcagggtgtt attttatgac gacgattctc aagcatctcc cggtaggtca acgtattggt
    30901 atcgcttttt ccggcggtct ggacaccagt gccgcactgc tgtggatgcg acaaaaggga
    30961 gcggttcctt atgcatatac tgcaaacctg ggccagccag acgaagagga ttatgatgcg
    31021 atccctcgtc gtgccatgga atacggcgcg gagaacgcac gtctgatcga ctgccgcaaa
    31081 caactggtgg ccgaaggtat tgccgctatt cagtgtggcg catttcataa caccaccggc
    31141 ggcctgacct atttcaacac gacgccgctg ggccgcgccg tgactggtac catgctggtt
    31201 gctgcgatga aagaagatgg cgtgaatatc tggggtgacg gtagcaccta taaaggaaac
    31261 gatatcgaac gtttctatcg ttatggtctg ctgaccaatg ctgaactgca gatttacaaa
    31321 ccgtggcttg atactgactt tattgatgaa ctgggcggtc gtcatgaaat gtctgaattt
    31381 atgattgcct gcggtttcga ctacaaaatg tctgtcgaaa aagcttactc cacggactcc
    31441 aacatgcttg gtgcaacgca tgaagcgaag gatctggaat acctcaactc cagcgtcaaa
    31501 atcgtcaacc cgattatggg cgtgaaattc tgggatgaga gcgtgaagat cccggcagaa
    31561 gaagtcacag tacgctttga gcaaggtcat ccggtggcgt tgaacggtaa aacctttagc
    31621 gacgacgtag aaatgatgct ggaagctaac cgcatcggcg gtcgtcacgg cctgggcatg
    31681 agcgaccaga ttgaaaaccg tatcatcgaa gcgaaaagcc gtggtattta cgaagctccg
    31741 gggatggcac tgctgcacat tgcgtatgaa cgcctgttga ccggtattca caacgaagac
    31801 accattgagc agtatcacgc gcatggccgt cagttgggcc gtctgctgta ccaggggcgt
    31861 tggtttgatt cccaggcgct gatgctgcgt gactctctgc aacgctgggt tgccagccag
    31921 atcactggtg aagttaccct ggagctgcgc cgtgggaacg attattcaat cctgaatacc
    31981 gtctcagaga acctgaccta caagccagag cgtctgacga tggaaaaagg cgactcggtg
    32041 ttctcgccag atgatcgtat cggtcaactg accatgcgca acctggatat caccgatacc
    32101 cgcgagaaac ttttcggtta tgccaaaact ggcctgcttt cctcctctgc cgcttcaggc
    32161 gtgccgcaga tggagaatct ggaaaacaaa ggccagtaat tcgacatcaa ccctgccctt
    32221 caggggtggg gttaccttgc ttatcctctg taaactcctt tctgagcatc acataatcga
    32281 aaacatcatg gtttgcatcc acaaccagtg agtctccttt atagtgtaca atcacttcct
    32341 caagcgtttg cgactgttcc ggctttgtta cccccatcca cgcattaata aggtaattat
    32401 tgtaagctgt cgaaaagatg ttgttttccg ttgtgcgatc cacaccatcc ccaagaacgg
    32461 ggctatacca gataaacatc ggaacatgat atgcctgctg gctggcctcc ctgcctccat
    32521 gaaaatagac gttcttcttc gtagggtcac gttccaggcc gtggtcggca aaatacatga
    32581 ccgaggcgcg gcgatctttt aataattcaa aaacctgacc tagcaaacta tctgtgtaat
    32641 gaatggagtt gtcatagcag gcatcctgat cgtcctgcgg ttgaaacacg gcgctggatt
    32701 gcggataggc gctacaagcc ggttcatggc ttccatttaa atgaagaaca atcagtttct
    32761 tttgctgcgt attttgctgt aatgcttgcg ataaatgcgg caacaacaat tcatcaaatc
    32821 ctctgacata aactgtttcc atggcacgca tggcgatact ggtaactgct gtaccattct
    32881 gccgaaaagc ggattgcgag cttagccaga aagtctgaaa tcctgcttga ttagccatat
    32941 taataatgtt gtcggggtaa ttatgaatgt catggctcaa aacagaatca gcagttaaag
    33001 aaaggggaac cgacagcgca gtgtaaggtg caccgcttat tgcttgatta aacagtttga
    33061 tctgttttct ttgtgcttcg acttgcggtg tcgtagagcg tgtatatcca tacaaagaca
    33121 tattgtcgac acgtacagac tcccccacaa tcaacacgta ggtatcaata cctgtatccc
    33181 tgactgataa ttgaaaatac ggcactgtgt ttgcaattga gagtaatctt tgatgctctt
    33241 tcgctgctaa agcaaaataa ttgagattga aaaacggcgt ataggtagca aatcgcgacg
    33301 ctagtatata tggactgaac gcatttttat ttttggcatc tttataagca aattgacaag
    33361 cggaaaataa acttcccgaa atgacaatca gcaataatat tcctgtcact ttttttgtcg
    33421 gcaaggaaac atcatatttt attattacag ccaaaaaaag aagggataaa aaggcaaaag
    33481 cacatagata agggctatac attcccagca ttttggcaac ttcatccgga tcactctgca
    33541 gcacactaat cgcaaatcca tcattgaaag ttgtaccaaa ggtacaccat gaatagaggc
    33601 taatactcat gtctgccgca gtcagtaaca caaatggtgc ggcaacaagc aggcgaagcg
    33661 gaagggatga tatacgttta acaaccagga tggtcagaac gaagaacagc aatcgctgaa
    33721 ccatatgcgc tccggaagag gcgactaagt tcgtaatacc aaaaacaata acactaagat
    33781 acaacaacca atgagattta aaacttctag caaatttgtt gaatactgtc atccagaaat
    33841 ccaagttcac aaccaatata aacaattatc aacaatgtgc aattttatat cagacatgtt
    33901 tttcaactct gatccatatc agaacgaaac actgttagtt ttttcttttt aaatgatgcg
    33961 gccattgcgt tctcagtaat taaataacat tatcaatgcg tctctaaaca ttacgcagaa
    34021 cgtagccatc agtagttata ctgacaggag caggatggtt tatcgagaag taaacatatc
    34081 cgtattcgta ccggaactgg agttgacaca ataaagtgcc aattatgtca gcagtaggga
    34141 aaagtttata acgaaggcgt accggcacca tcgtttcaag gtaccagcta cgagtaaagc
    34201 aactggacga gatacagata cctgacaacc attcctcaga ccaggaccaa aacgaaaaaa
    34261 gacgcttttc agcgtctctt ttctggaatt tggtaccgag gacgggactt gaacccgtaa
    34321 gccctattgg gcactaccac ctcaaggtag cgtgtctacc aattccacca cctcggcacg
    34381 gaaactactt tttagttcgg gatatcgctg gtcggcttag ccggagcagc tggctgagtt
    34441 tgttcggttt tcgccggcgc actcaggttt tcccattcgc tacctttatt ggtcttgttg
    34501 ctattgatgt tacccagcac cagactgatg atgaagaata acgttgccag caacgccgtc
    34561 atgcgggtca tgaagttacc agaaccactt gaaccaaaca gcgtagcgga agcgcctgct
    34621 ccgaaggagg ctcccatatc agcgccttta ccttgctgta gcatgatcag accaacaagg
    34681 ccaattgcca caataaggaa aactaccaaa agagcttcgt acataatcaa cctgttcctt
    34741 gcggagttgc cgcgtaccaa tgcttcaacc aataaagcgg gagtttataa tctttcccac
    34801 tgaagcgggt gtgaatacta accaaaacga atgaccttcg caagggcaat tttatcgcat
    34861 tgtatcaact gcggaaaaaa acagcaaaac cgatgtgttc gccgagaaaa aaggcgacaa
    34921 tcgccgcctt tttagccagt tatctaacgc tttaaacggc ttttactgca tcggcgatgc
    34981 ggtgtgcaaa ttcagtcacc tgcgcttcgt cttcgccttc caccatcacg cgaattaacg
    35041 gttcggtgcc ggatttacgc aacagcacac gtccacggct acccagcgcc gtttcaactt
    35101 ctgcggtcac cgctttgact gattcatgct ccagcggatc gccgctacct gcggtgtaac
    35161 gcacgtttac caggatttgc gggaacattt tcatgccgct gcaaaggtcg tgcaggctca
    35221 tatggttacg cgccatcgcc gccagcacct gcaaaccagc aacgatgccg tcaccggtag
    35281 tggttttatc cagcaggatc acatgaccgg aattctctgc accgatacgc cagcctttct
    35341 cctgcatttt ttccagtacg tagcggtcac ccactttcgc gcgcgcaaat ggaattccta
    35401 actgtttcag cgccagttca agccccatgt tgctcatcaa tgtacccaca gcgccaccac
    35461 gcagctggcc ctgacgaaga ccttcacgcg cgatgatata catgatctga tcgccatcga
    35521 ctttattgcc ttcatggtca accataatca cgcgatcgcc gtcgccgtcg aaggcaatac
    35581 cgagatccgc tttttcagcc agcacacgag cctggagcgc gcgaacgtcg gtagccccca
    35641 cttcggcatt gatgtttaca ccgtttggct cacaaccgat agcgataaca ttcgctccca
    35701 gttcgcgcag cacgttcggc gcgatgtgat aagtcgcacc gtttgcgcaa tccaccacaa
    35761 tcttcagttc actgaggcta agttcgttcg ggaacgtggc tttgcaaaac tcgatatagc
    35821 gacccgcagc atcaacgata cggctggctt tacccagttc tgccgaatca acacagctga
    35881 tctctttttc catttccgct tcgatggctt cttcaactgc atccggcagc ttagtaccgt
    35941 caatagaaaa gaatttaatg ccattatcgt agaacgggtt atgcgatgca gatatcacaa
    36001 ttccggcctc tgcgcggaag gtacgcgtca gataagccac ggccggtgtt ggcatcgggc
    36061 cagtgaagag tgcggaaagg cccgcagccg ccagccccgc ttccagtgct gactccagca
    36121 tatagccaga aatacgcgtg tctttaccaa taataatctt acgggagccg tggcgcgcca
    36181 gcactttacc cgcggcccaa cccagcttaa gcacaaaatc aggtgtgatc ggcgcatccc
    36241 ctacacgacc acgaatccca tcggtaccga aatatttacg attactcata gcgtttgttt
    36301 tcctttgcag acagagtagc ttccaccact cgcatcgctt ctacggtttc tttgacgtca
    36361 tgaacacgaa taatgtgcgc gccttgcatt gcggcaatga ccgcacaggc cagactaccg
    36421 ctcaggcgct cggacggccc cacgttcagc agctgcccaa tcatcgattt tcgtgacata
    36481 cccaccaaca gcggcaggtt gaaatggtga aattcagcca ggcgcgccag taatgaatag
    36541 ttatgggaga gatttttacc gaaaccgaat ccggggtcga gcaacaattt ctcttttgcg
    36601 atacccgcct gctcgcaacg tgctatttgc tcaataaagt agcgattcac ttctgcaaag
    36661 acatcgtcat acttcggggc ttcctgcatg gtttttggat ttccctgcat atgcatcaga
    36721 caaaccggta aaccggtttc tgcagccgcc tccagagcgc caggttcgga aagggagcgg
    36781 atatcgttaa taatgtgagc gccaactttc gctgactcac ggatgacttc tggtttggat
    36841 gtatcgaccg agatccagac ttcgaagcgt tgagcaattg cctcaaccac aggaataaca
    36901 cgttgcaact cttcttcaac gctaacttcc gccgcccctg gccgcgtgga ctcgccacca
    36961 acgtcaatga tcgtcgcgcc agcgttgatc atcagattcg catgtttcac cgcatctatc
    37021 agcgagttat gcgtgccacc atccgaaaag gaatcaggcg tgacgttgag gatccccatt
    37081 acgtgaggat ggctaaggtc cagtgaagta ccctgggcaa agagtttcat gatgttatcc
    37141 ctggtatgaa ttgataagaa aaaaccccgg agcacgcccc ggggttttcg gtacaaatac
    37201 agtcatctga tgcgggaact tacttgtcgc ctaactgctc tgacatggtg ttacccgggt
    37261 tcggcgtacg cggttcatca accggacgag gagcctttgg actaccattg tcgccagcat
    37321 tgttagaagc gcctggttct tcccagcccg ctggcggacg tacatcgcga cgtgccatca
    37381 ggtcatcaat ctgcggtgcg tcgatagtct catatttcat gagagcatct ttcatcgcat
    37441 gcagaatatc catattgtcg gtcagaagct gacgcgcacg attatagtta cgctcaatca
    37501 gtgctttcac ttcctggtcg atgatacgtg cagtttcatc ggacatatgt ttcgctttcg
    37561 ctacgctacg gccgaggaac acttcacctt cttcttccgc gtacagcagt ggacccaatt
    37621 tctcagagaa gccccactga gtcaccatgt tacgtgccag gttggtcgca actttaatat
    37681 cgttggacgc accggtagaa acatgttccg gcccgtagat gatctcttct gccagacgac
    37741 caccgtacag cgtagaaatc tggctttcca gtttctgacg gctggcgctg attgcgtcgc
    37801 cctcaggcaa gaagaaagtc acacccagcg cacgaccgcg tgggataatc gtcactttgt
    37861 gcaccggatc gtgctccggc accaggcgac cgataatcgc atgacccgct tcgtggtaag
    37921 ccgtcgattc tttctgcgct tccgtcatca ccatggagcg acgttccgca cccatcatga
    37981 ttttgtcttt cgctttctcg aactcaacca tcgacacaac gcgtttgttg ccacgagcag
    38041 cgaacagtgc cgcttcgttc accaggttcg ccaggtcagc accggagaaa ccaggagtac
    38101 cacgggcaat gattgccgcg tcgatatcgg gtgccaatgg tacgcgacgc atgtgaacct
    38161 tcaggatctg ctcacgaccg cgaacatctg gcaagccaac cacaacctga cggtcgaaac
    38221 ggccaggacg cagcagcgct gggtcgagaa cgtccggacg gttagtcgcg gcgataacga
    38281 tgataccttc gttaccttcg aagccatcca tctcaaccag catctggttc agagtctgtt
    38341 cacgttcatc gtgaccaccg cccagacctg cgccacgctg gcggcctacg gcgtcgattt
    38401 catcgataaa gatgatgcac ggtgccgctt tcttcgcctg ttcgaacatg tcacgaacac
    38461 gggatgcacc cacaccgacg aacatttcta cgaagtcaga accggagata gtaaagaacg
    38521 gaactttcgc ttcgcctgca atcgctttcg ccagcagcgt tttaccggta cccggaggac
    38581 cgaccatcag gacgcctttc gggatcttac cgccgagttt ctggaagcgg ctcggctcgc
    38641 gcagatactc aaccagttca gcaacttctt cttttgcttc gtcgcagccc gcaacgtcag
    38701 caaaggtcgt tttgatctga tcttccgtca gcatgcgcgc tttactctta ccaaacgaca
    38761 tggcaccttt gccaccgccg ccctgcattt gacgcatgaa gaagatccag acaccaatca
    38821 gcaacagcat cgggaaccag gagatgaaga tagaagccag caggctgggt tcttcaggcg
    38881 gttcaccgac aaccttgacg ttcttggtca acaggttatc cagtaatttc ggatcctgaa
    38941 ccggaatgta agtggtataa cggttactat ctttcttggt aacgttgatt tcacgtccgt
    39001 tgatacgcgc ttcacgaacc tggtcgttat tgacctcttg taggaaggta gagtaatcca
    39061 ccttacggcc attagactcg ctgggcccaa agctctggaa tactgacatc agcacaacgg
    39121 caatgaccag ccagagtatt aggtttttcg ccatgtcact caagggatta acctcttatt
    39181 acaactgtgt taaaaacagc gtcaggatac tctatatcca gcatctttca aactttcgtc
    39241 tgaaatctcc cggttagggt ttacgcccgg tcgctacaat atacacttcc cgcgaacgtg
    39301 cacgagaaga gtccggctta cgaactttga ccttcgtaaa cagggagcga atttccctta
    39361 gatactcatc gaaaccttcg ccctggaaca ccttcactac aaaactgcca cctggcgcta
    39421 atacatcacg acacatttct agcgccagtt ccaccagata catggcacgg gggatatcca
    39481 ccgccggtgt tccgctcatg tttggtgcca tatcggacat gacaacctgg actttgctgt
    39541 cgccaacgcg ctccagcagt gctttcatca ccagttcatc acgaaaatcg ccctgaagaa
    39601 agtccacacc aacgatagga tccataggta aaagatcgca agcgatgatg cggcctttgc
    39661 cgccaatttg ggtgaccaca tattgtgacc aaccacccgg agcagcacca aggtcgacaa
    39721 ccgtcattcc cggtttaaag agtttgtcac tttgctgtat ttcatcaagt ttaaaccagg
    39781 cacgggaacg taaccccttt ttctgtgcct gttgaacata tttatcgcta aagtgttcct
    39841 gaagccagcg gctggagctg gcagaacgct tcttacctgt catttaactt tcccatgggg
    39901 ataactcatc gtaaccaatt gcgtaaattt ttacgcgcct atttggtgat atatgggaga
    39961 tggcggtaga atgacccgtt ttcaatccca acgtaagcaa aaaatacgat gaatctgagt
    40021 actaaacaaa aacagcacct gaaaggtctg gcacatccgc tcaagccagt tgttctgctt
    40081 ggcagtaatg gtttgaccga aggggtgctg gccgagattg aacaagcgtt agagcaccat
    40141 gaactcatca aggtgaaaat cgccaccgaa gatcgcgaaa ctaaaacctt gatcgtggaa
    40201 gctatcgtgc gcgaaaccgg cgcctgtaat gtacaggtca tcggtaaaac gctggtgctt
    40261 tatcgcccaa ctaaagaacg taaaatctcg ctgccacgct aagattatcc taaagttaca
    40321 cacatttgct gtgtaaaacg agggggtttc cgcaggcagg agagcaaaat gccacgctct
    40381 gttcgttgat aaaaggccgc tatgcggcct ttttcctttc tttacaatac atcaacatct
    40441 tgagtattgg gtaattctta caggtattcc accttaatta cttcaaattc tacttcgccg
    40501 cccggcgttt tgatgaccac aacatcatct tcttctttgc cgatcaggcc acgagcaata
    40561 ggtgagttta cagaaatcag gttttgttta aagtcagctt cgtcatcgcc aacgatgcga
    40621 taagtctgtt cttcgtcaga atcgagattc agcaccgtta cggtagcacc aaaaataacg
    40681 cgcccattgt tgggcatttt ggtgacatca atcacctgcg cgttcgacag cttggcttcg
    40741 atgtctttga tacggccttc gcagaagccc tgctgttcac gagctgcgtg gtattcggcg
    40801 ttttctttca ggtcgccatg ctcacgcgct tccgcgatag cagcaatgat ttcaggacgg
    40861 cgcacagatt tcagaaaatc cagctcttcg cgtaattttt cagcgccgcg taaggtcatc
    40921 ggaatagctt gcatttgtta tacctcttga atattcctga tggggcaagt gttcacccac
    40981 cccggctgtt cggcctgccc ggcataactc ctcaccggga ccagaagcaa aaaaataccg
    41041 acccgggtac aagtcccagg tcagctacaa ttcacatttt gatagtcatt ttaccctgaa
    41101 gttcccgaag ggtcatcgtt tactttatag ggcgttgcgc cgtagtatga cggctcgatt
    41161 ccaggttgtt agcgcgagat tatgcgattt tccagattta tcatcggatt gaccagctgt
    41221 atagcgttca gtgttcaggc cgcaaatgtt gatgagtaca ttactcaact ccccgctggt
    41281 gccaaccttg ccctgatggt gcaaaaagtc ggcgcgtcgg cccccgctat tgattaccac
    41341 agtcagcaga tggcgctgcc cgccagtacc cagaaagtga ttactgcgct ggcggcgttg
    41401 attcaactcg gccccgattt tcgtttcacc acgacgcttg aaaccaaagg caatgtggaa
    41461 aacggcgtac ttaagggtga cttagtggcg cgatttggtg ccgatccgac gttaaaacgt
    41521 caagatattc gcaatatggt cgcgactttg aaaaaatctg gcgtcaacca aatcgatggc
    41581 aatgtgttga tagatacctc cattttcgcc agccacgata aagcccccgg ctggccatgg
    41641 aatgacatga cacaatgctt tagcgctccg cctgccgtcg ccatagttga ccgcaactgt
    41701 ttctccgtct cgctctacag tgccccaaaa cctggtgata tggcttttat acgcgtggca
    41761 tcttattacc ccgttacgat gttcagccag gtacgcaccc tcccccgtgg ttctgccgaa
    41821 gcgcaatact gcgaactgga tgtggtgcca ggcgacctga accgctttac gctgacggga
    41881 tgcctgccac aacgttctga gccgctcccg ttggcttttg ccgtgcagga tggagccagc
    41941 tatgccggtg caattctgaa agatgagtta aaacaggcgg gtatcacctg gagcggaaca
    42001 ctgctgcgcc agactcaggt taacgaacct ggaacggtag ttgccagtaa acagtcggcc
    42061 ccgctgcacg atctgcttaa gattatgctg aaaaagtcgg acaacatgat cgccgatacg
    42121 gttttccgca tgataggcca tgcgcgcttc aatgtgcctg gaacatggcg ggccgggtcg
    42181 gacgctgtgc gtcagatcct gcgccagcaa gccggtgtcg atattggaaa caccattatt
    42241 gccgatggtt cagggctttc gcggcataac ctgattgccc ccgccaccat gatgcaggtg
    42301 ctgcaataca ttgcccaaca cgacaatgaa cttaacttta tctccatgct gccgctagcg
    42361 ggctatgacg gctctttgca gtaccgtgca ggtctgcatc aggcgggcgt ggatggaaaa
    42421 gtctcagcga aaaccggttc gttgcagggg gtatataacc tggcgggatt cattaccaca
    42481 gcgagcgggc aacgaatggc gtttgtacaa tatctttctg gctatgcagt agaaccggcg
    42541 gatcagcgta atcgccgtat tccgttagtg cgttttgaaa gccgtttgta taaagatatt
    42601 tatcagaaca attagtcaaa aagaaacccc ggcacatggc cggggttttg ctgattaacg
    42661 cttgtaaatg aactcaacgc cttcttcgtc gtcttcgtcc cagtcgtcat cccagtcctc
    42721 atcatcctct tcagcaatct cttcaagctg ctggcgatga taatcatccc acatgaattc
    42781 gactttctct ggctgtttcg cttcttcagc ctgcacgacc gggttttcaa tgataaaggt
    42841 catcacatcc cagcagagat ctttcacgcc cagtccactc gccgcagaga tcagataata
    42901 tttatcttcc cagcccagcg cctctgcgat cgctttcgct ttctcttcgg cttctacctt
    42961 atccagcaga tcgatcttgt tgaagactaa ccaacgcggt ttcgccgcca gatcctggct
    43021 gtatttttcc agctcgctga taataatacg cgcgttttca accggatcgg tgccgtcaat
    43081 cggatcgata tcgatgaggt gcaacaggac gcggcaacgt tccaggtgct tcaggaagcg
    43141 aatgcccaga cctgcgcctt ccgcagcgcc ttcaatcagt cctggaatat cggcaacaac
    43201 gaagctcttt tcgttgtcca ttcgtaccac acccagactt ggcaccagag tggtaaacgg
    43261 ataatccgcc actttcggtt tagccgccga taccgcacga ataaaggtcg atttacccgc
    43321 gtttggcatc cccaacatac cgacgtcagc cagcagcatc agctccagca gcagctcacg
    43381 cttatcgccc ggcgtaccgt tagttttctg ccgtggtgta cggttaactg acgatttgaa
    43441 acgggtattg cccagaccgt gccagccgcc cttagcaacc agcagacgct gaccgtgttt
    43501 ggtcatatcg cccatggttt caccagtacc ctggtcgatt acacgcgtac ctaccggcac
    43561 tttaattgtg acgtctttac cgcgcttacc ggtacagtcg cggcttgcgc cattctgacc
    43621 gcgctctgca cggaaagatt tttcaaaacg ataatcgata agcgtgttca ggttctcgtc
    43681 ggcttccatc catacgtcac caccatcacc gccgtcgccg ccatccgggc cgcctttcgg
    43741 aatatacttt tcgcggcgga agctcacgca accattaccg ccatcacctg caacgaccag
    43801 aatcgatgct tcatcaacaa acttcatttt attctccgta aatcattcgc ctgcgcgggg
    43861 ttgcgatacc accgttgtat gcttacgtaa tccgccccaa atacgatgac caatggcgga
    43921 atacatcgcg cccgcaacca cgacaaacgc accgagataa cctaaaaggt ttaacatcgg
    43981 tctggcgaag aaatcgggcc aggccagtga taaaagatct gaaaaaaaca gcgtaaacag
    44041 tggggtgagc gtgatgatcg cgctcacctg cgctgcctgc cagcgagcca tcgcttccgc
    44101 cagggcgcca tatcctacca aggtattcag tccgcaaaaa attaaacatg cgagctgcca
    44161 gtggctaagc tgcgctatca ctccaggctt tgccagaggg aagagcgcaa ttgtacataa
    44221 agtgtacagt aaaaacagga tctgcggtga ggccagccga cgcaataaaa ccttttgcgc
    44281 cacgccataa ctcacccaaa ccgtcgcagc accgacccca aagataactc cccaggtgta
    44341 atcggttagc tttgtaaata tctcgaccag actggtgtta aaaaacatca ccaggccgct
    44401 caggagcatc aatgccccta caacctgagt gctgcgcatt ttctctttca ggataaatac
    44461 gctggcaacc atcatgccaa ctggcgagag ttgcccaatc acctgcgaag cggtcggact
    44521 cagggatttc aaggatgagc tgaacaggat gaagttccca aacagcccgg cggtcgccac
    44581 tgccaacaaa atcaaccagc gtggcttacg aaacacgcgt aatggcggca acctcttctt
    44641 caccgcaaga atggccccca ggccaatact cgccatcaag aaacggtaaa acacgattgt
    44701 cggaggttcc atcacctcca gcacctgctt cattgcaatt ggcaacgccc cccagcaaat
    44761 tgctgtggtg agcgccaaaa gaatgccaat gcctgcctgc tgcttcatac cgtattccct
    44821 gcaaagcgca tttgcggatt aacgtcgcac catgcgcatt tttaccggtt atcgaatgta
    44881 aaaagccccg caacgtgttg cggggctttc atccgttacc gggacgcgaa aaacttattc
    44941 agcttcgatg ctgataaatt tacggttttt cgggccttta acttcgaatt tcactttacc
    45001 gtctgcttta gcaaacagag tgtggtcacg accgcaacct acgttagcgc cagcgtggaa
    45061 tttggtacca cgttgacgaa cgatgatgct acccgccaga acggattcgc caccgaaacg
    45121 cttaacgccc aggcgtttag cttctgaatc gcgaccgtta cgtgtggagc cgccagcctt
    45181 tttatgtgcc atttgaaatc tctcctcagg tcttaggcgc tgatgccagt aattttcaca
    45241 tcagtgaacc actgacgatg gccctgctgc ttacgatagt gtttacgacg acgaaactta
    45301 acgattttaa ctttctcgcc acgaccgtga gcaacaactt cagctttgat tacgccgcca
    45361 tcaacgaaag gaacgccgat tttgacttct tcaccgtttg cgatcatcag cacttcagcg
    45421 aactcaacag tttcgccagt tgcgatgtcc agcttttcca ggcgaacggt ctgaccttcg
    45481 cttactcggt gttgtttacc accactttgg aaaaccgcgt acatataaaa ctccgcttcc
    45541 gcgcacacct tttcaatgat tcagagtgcg ctataaatat tcacaatagg gcgcgaatat
    45601 tacgcaaaac gcacgccttt gacaagtgct acagtcaata cacgaagaaa aaaaacacaa
    45661 cttgtacggt aacgtttatc tgtgccattt tttcagtaca atcaccctat attcctaacc
    45721 ataaacccta agttgccttt gttcacagta aggtaatcgg ggcgaaaagc ccggcttttg
    45781 cgatgaattt agaaaaaatc aatgagttaa ccgcgcaaga tatggcgggt gttaatgcgg
    45841 caatccttga gcagcttaat tccgacgtcc aactgatcaa tcagttaggc tattacatcg
    45901 tcagcggcgg cggtaaacgt attcgtccga tgattgctgt actggctgca cgagctgttg
    45961 gctatgaggg aaatgcgcat gtcaccattg ctgccctgat cgagtttatc cacacggcga
    46021 ctctgctgca cgacgacgtt gtggatgaat cagatatgcg caggggtaaa gctaccgcca
    46081 acgccgcatt tggcaatgcc gccagcgtgc tggtaggcga ttttatttat acccgcgcgt
    46141 tccagatgat gaccagcctc ggttcactca aagtgctgga agtcatgtca gaagccgtaa
    46201 acgtcatcgc agaaggtgaa gttctgcaac tgatgaatgt taacgatccg gacatcactg
    46261 aagaaaacta catgcgcgtt atctatagca aaaccgcgcg tctgtttgag gctgccgcgc
    46321 agtgttccgg aattctggcg ggctgtacgc cggaggagga gaaaggcctg caggattatg
    46381 ggcgctatct cggcactgct ttccagttga tcgacgattt actcgattac aatgccgatg
    46441 gcgaacagtt aggtaaaaac gtcggcgacg atctgaacga aggtaaaccg acgctgccgc
    46501 tgctgcatgc gatgcatcat ggcacaccag aacaggcaca gatgatccgt accgccatcg
    46561 aacagggtaa cggtcgccat cttctgaaac cggttctgga agcaatgaac gcttgtggat
    46621 ctcttgaatg gacgcgtcag cgcgccgagg aagaagcaga caaagccatc gcagcgttac
    46681 aggtgctccc ggacacccct tggcgagaag cactcatcgg cctcgcgcac atcgctgttc
    46741 aacgcgatcg ttaatcccct cccctcatcc cgcgcagtgc gcgggatgat tccagtaaac
    46801 tccataagaa tacctattca gcgtaataat gc
//
LOCUS       DBFIFI010000015        46408 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.015, whole genome shotgun sequence.
ACCESSION   DBFIFI010000015 DBFIFI010000000
VERSION     DBFIFI010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 46408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46408
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.015"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(14..727)
                     /gene="lpxT"
                     /locus_tag="N7B39_000858"
     CDS             complement(14..727)
                     /gene="lpxT"
                     /locus_tag="N7B39_000858"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HGU2420883.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            complement(766..1752)
                     /gene="yeiR"
                     /locus_tag="N7B39_000859"
     CDS             complement(766..1752)
                     /gene="yeiR"
                     /locus_tag="N7B39_000859"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HGU2420884.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVAGHLLDLPRHNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGHQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            complement(1870..3336)
                     /gene="yeiQ"
                     /locus_tag="N7B39_000860"
     CDS             complement(1870..3336)
                     /gene="yeiQ"
                     /locus_tag="N7B39_000860"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HGU2420885.1"
                     /translation="MNTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQAIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLDE
                     QAPTLRIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRDLVAGSISEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQARQQIAQFGAHQALLNTLKI"
     gene            complement(3559..4131)
                     /gene="yeiP"
                     /locus_tag="N7B39_000861"
     CDS             complement(3559..4131)
                     /gene="yeiP"
                     /locus_tag="N7B39_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HGU2420886.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            4286..4540
                     /gene="yeiW"
                     /locus_tag="N7B39_000862"
     CDS             4286..4540
                     /gene="yeiW"
                     /locus_tag="N7B39_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HGU2420887.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            complement(4537..5718)
                     /gene="setB"
                     /locus_tag="N7B39_000863"
     CDS             complement(4537..5718)
                     /gene="setB"
                     /locus_tag="N7B39_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HGU2420888.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGVLVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            6086..7216
                     /gene="fruB"
                     /locus_tag="N7B39_000864"
     CDS             6086..7216
                     /gene="fruB"
                     /locus_tag="N7B39_000864"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HGU2420889.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            7216..8154
                     /gene="fruK"
                     /locus_tag="N7B39_000865"
     CDS             7216..8154
                     /gene="fruK"
                     /locus_tag="N7B39_000865"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HGU2420890.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            8171..9862
                     /gene="fruA"
                     /locus_tag="N7B39_000866"
     CDS             8171..9862
                     /gene="fruA"
                     /locus_tag="N7B39_000866"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HGU2420891.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATSE
                     GKNESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            10286..11227
                     /gene="psuK"
                     /locus_tag="N7B39_000867"
     CDS             10286..11227
                     /gene="psuK"
                     /locus_tag="N7B39_000867"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HGU2420892.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRERLSQIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            11215..12153
                     /gene="psuG"
                     /locus_tag="N7B39_000868"
     CDS             11215..12153
                     /gene="psuG"
                     /locus_tag="N7B39_000868"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HGU2420893.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEHTINAVIDQAVAEAEAQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            12247..13497
                     /gene="psuT"
                     /locus_tag="N7B39_000869"
     CDS             12247..13497
                     /gene="psuT"
                     /locus_tag="N7B39_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HGU2420894.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVAAALLLQIALGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMSVPIDYLLAASLMAIPGGILFAR
                     ILSPATELSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(13568..14266)
                     /gene="yeiL"
                     /locus_tag="N7B39_000870"
     CDS             complement(13568..14266)
                     /gene="yeiL"
                     /locus_tag="N7B39_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HGU2420895.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            14396..15337
                     /gene="rihB"
                     /locus_tag="N7B39_000871"
     CDS             14396..15337
                     /gene="rihB"
                     /locus_tag="N7B39_000871"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HGU2420896.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGDTGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDSDWFWGLVEECVRGYIKTH"
     gene            15437..16687
                     /gene="nupX"
                     /locus_tag="N7B39_000872"
     CDS             15437..16687
                     /gene="nupX"
                     /locus_tag="N7B39_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HGU2420897.1"
                     /translation="MDVMRSVLGMVVLLTIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESIVGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(16743..17831)
                     /gene="yeiI"
                     /locus_tag="N7B39_000873"
     CDS             complement(16743..17831)
                     /gene="yeiI"
                     /locus_tag="N7B39_000873"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HGU2420898.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGMADIRYPQSASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDET
                     VLAINDTHLLEQLSPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLAGEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(17834..18691)
                     /gene="nfo"
                     /locus_tag="N7B39_000874"
     CDS             complement(17834..18691)
                     /gene="nfo"
                     /locus_tag="N7B39_000874"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HGU2420899.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARTVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(18765..19814)
                     /gene="yeiH"
                     /locus_tag="N7B39_000875"
     CDS             complement(18765..19814)
                     /gene="yeiH"
                     /locus_tag="N7B39_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HGU2420900.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            19913..20794
                     /gene="yieE"
                     /locus_tag="N7B39_000876"
     CDS             19913..20794
                     /gene="yieE"
                     /locus_tag="N7B39_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HGU2420901.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            20999..22468
                     /gene="lysP"
                     /locus_tag="N7B39_000877"
     CDS             20999..22468
                     /gene="lysP"
                     /locus_tag="N7B39_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HGU2420902.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPGKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            22760..24739
                     /gene="cirA"
                     /locus_tag="N7B39_000878"
     CDS             22760..24739
                     /gene="cirA"
                     /locus_tag="N7B39_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HGU2420903.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFTA
                     NGVPVFSYYNVNKARIQGVETELKIPFNDEWKLSLNYTYNDGRDVSNGENKPLSDLPF
                     HTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVTKDVK
                     LRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(24771..25607)
                     /gene="yeiG"
                     /locus_tag="N7B39_000879"
     CDS             complement(24771..25607)
                     /gene="yeiG"
                     /locus_tag="N7B39_000879"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HGU2420904.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSRYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            25865..26533
                     /gene="folE"
                     /locus_tag="N7B39_000880"
     CDS             25865..26533
                     /gene="folE"
                     /locus_tag="N7B39_000880"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HGU2420905.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            26550..27707
                     /gene="yeiB"
                     /locus_tag="N7B39_000881"
     CDS             26550..27707
                     /gene="yeiB"
                     /locus_tag="N7B39_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HGU2420906.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVIINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMQFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGPAISKTSR"
     gene            27849..28889
                     /gene="galS"
                     /locus_tag="N7B39_000882"
     CDS             27849..28889
                     /gene="galS"
                     /locus_tag="N7B39_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HGU2420907.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYTHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            29168..30166
                     /gene="mglB"
                     /locus_tag="N7B39_000883"
     CDS             29168..30166
                     /gene="mglB"
                     /locus_tag="N7B39_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HGU2420908.1"
                     /translation="MNKKVLTLSAVMASMLFGATAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            30227..31747
                     /gene="mglA"
                     /locus_tag="N7B39_000884"
     CDS             30227..31747
                     /gene="mglA"
                     /locus_tag="N7B39_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HGU2420909.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYKKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKKINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKRDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            31763..32773
                     /gene="mglC"
                     /locus_tag="N7B39_000885"
     CDS             31763..32773
                     /gene="mglC"
                     /locus_tag="N7B39_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HGU2420910.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQAPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(33016..34251)
                     /gene="preA"
                     /locus_tag="N7B39_000886"
     CDS             complement(33016..34251)
                     /gene="preA"
                     /locus_tag="N7B39_000886"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HGU2420911.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(34245..35483)
                     /gene="preT"
                     /locus_tag="N7B39_000887"
     CDS             complement(34245..35483)
                     /gene="preT"
                     /locus_tag="N7B39_000887"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HGU2420912.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLLRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDDFAKLEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(35677..35916)
                     /gene="yeiS"
                     /locus_tag="N7B39_000888"
     CDS             complement(35677..35916)
                     /gene="yeiS"
                     /locus_tag="N7B39_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HGU2420913.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(35919..36638)
                     /gene="sanA"
                     /locus_tag="N7B39_000889"
     CDS             complement(35919..36638)
                     /gene="sanA"
                     /locus_tag="N7B39_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HGU2420914.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(36788..37672)
                     /gene="cdd"
                     /locus_tag="N7B39_000890"
     CDS             complement(36788..37672)
                     /gene="cdd"
                     /locus_tag="N7B39_000890"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HGU2420915.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LADAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHNIDRVLLA"
     gene            complement(37802..38497)
                     /gene="yohK"
                     /locus_tag="N7B39_000891"
     CDS             complement(37802..38497)
                     /gene="yohK"
                     /locus_tag="N7B39_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HGU2420916.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APILFPIILAVMG"
     gene            complement(38494..38892)
                     /gene="yohJ"
                     /locus_tag="N7B39_000892"
     CDS             complement(38494..38892)
                     /gene="yohJ"
                     /locus_tag="N7B39_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HGU2420917.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAI
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            complement(39023..39931)
                     /locus_tag="N7B39_000893"
     CDS             complement(39023..39931)
                     /locus_tag="N7B39_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGU2420918.1"
                     /translation="MANWAQKLKLHHLEMLVALGEQGNLTHVARMMNITQPALSKWLS
                     QLEDEIGITLFERHSKGLRPTEGGKLLLQHAQRLINDMERSQHELARFKQGGLVGSLK
                     IGCSPVATDCVSQAILHLLTKMPTLHLNIEEKVMTPLLQDLLAGSVDVVVGRIGGRAL
                     QLPLNYQVLYTEPVCFVARPEHPLAKYATLSWNDLANWRWIVWPTGTPIRISIDNALV
                     DHGVMLPENTIESASMNVSTNLLQSSDMISILSLRLAQRYASQGQLVILNLPKIEQKG
                     SVGVFWRNNETPSYALSQFLHFLQQN"
     gene            40058..41416
                     /locus_tag="N7B39_000894"
     CDS             40058..41416
                     /locus_tag="N7B39_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2420919.1"
                     /translation="MTQRRDLQALIDAAPVGKMQWRVIICCFLVVMLDGFDTAAIGFI
                     APDIRTHWQLTAGDLAPLFGAGLLGLTAGALLCGPLSDRFGRKRVIELCVFLFGALSL
                     ASAFSPDLQTLVFLRFLTGLGLGGAMPNTITMTSEYLPARRRGALVTLMFCGFTLGSA
                     FGGIVSAQLVPVIGWHGILVLGGVLPLMLFVALLVVLPESPRWQVRRQLPQAVIAKTV
                     SAITRERYVDTHFYLIESASVTKGSIRQLFMGRQLPITLMLWVVFFMSLLIIYLLSSW
                     MPTLLNHRGIDLQHASRVTAAFQIGGTLGALALGVLMDKFNPFRVLTLSYAIGAICIV
                     MIGLSQDGLWLMALAIFGTGIGISGSQVGLNALTATLYPTQSRATGVSWSNAIGRCGA
                     IVGSLSGGVMMAMNFSFDTLFFIIAVPAAISAVMLTLLITVVRQSTSVPDSLPRAGVV
                     NE"
     gene            41428..42456
                     /gene="gtdA"
                     /locus_tag="N7B39_000895"
     CDS             41428..42456
                     /gene="gtdA"
                     /locus_tag="N7B39_000895"
                     /EC_number="1.13.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047922 - gentisate 1,2-dioxygenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gentisate 1,2-dioxygenase"
                     /protein_id="HGU2420920.1"
                     /translation="MTDNNQNSREQFYQHISGQNLTPLWESLHHLVPKTPNANCAPAY
                     WNYQEIRPLLLESGGLIGAKEAVRRVLVLENPALRGQSSITATLYAGLQLIMPGEVAP
                     SHRHNQSALRFIVEGKGAFTAVDGERTPMNEGDFILTPQWRWHDHGNPGDEPVIWLDG
                     LDLPLVNILGCGFAEDYPEEQQPVTRKEGDYLPRYAANMLPLRHQTGNSSPIFNYRYD
                     RSREVLHDLTRLGDADEWDGYKMRYVNPVTGGYPMPSMGAFLQLLPKGFASRVARTTD
                     STIYHVVEGSGQVIIGNETFSFSAKDIFVVPTWHGVSFQTTQDSVLFSFSDRPVQEAL
                     GLFREARY"
     gene            42471..43172
                     /locus_tag="N7B39_000896"
     CDS             42471..43172
                     /locus_tag="N7B39_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HGU2420921.1"
                     /translation="MTQYVFEPQAPVTVPVVGSDEQFPVRRVYCVGRNYAAHAREMGF
                     DPDREPPFFFCKPADAVVPVAAGETLALPYPAQTDNYHYEIELVVAIGKKGSDIPLEH
                     AHEYVWGYATGLDMTRRDRQMEMRQMGRPWEIGKAFDLSAPIAPLHKARDIGGIDNAP
                     IWLQVNGEDHQRSDIRHLIWSVNETICYLSGFFELQPGDLIFTGTPEGVGPVVKGDVI
                     TGNVEGLTPIAVKIV"
     gene            43181..43825
                     /gene="maiA"
                     /locus_tag="N7B39_000897"
     CDS             43181..43825
                     /gene="maiA"
                     /locus_tag="N7B39_000897"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311055.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HGU2420922.1"
                     /translation="MKLYSFFNSSASYRVRIALALKGINYQTEGVNIRIGQQNELAYR
                     RMNPVGLVPTLLTDEGESLGQSLAIIDWLERHYPQVPLVPQEEPARNKVLEIVYAIAC
                     DIHPLNNLRVLRYLTEELNVSEEEKKRWYAHWIQQGLSAVEQLLRQNQSGQFCVGETP
                     TLADCCLVPQWANALRMNCDLSGYPRCKAVYDACTQLPAFIAAAPENQQDKISA"
     gene            43840..45033
                     /locus_tag="N7B39_000898"
     CDS             43840..45033
                     /locus_tag="N7B39_000898"
                     /EC_number="1.14.13.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybenzoate 6-monooxygenase"
                     /protein_id="HGU2420923.1"
                     /translation="MTKVTRAVIVGGGIGGAATALSLARLGIKVVLLEKAHEIGEIGA
                     GIQLGPNAFSALDSLGVGEVARQRAVFTDHITMMDAVNAEEVVCIETGQAFRDHFGGP
                     YAVIHRVDIHASVWEAVLTHPDVEYHTSTNVVDIRETEDDVTVFDDRGNSWTGDILIG
                     CDGVKSVVRQSLLGDAPRVTGHVVYRAVIDRADMPEDLRINAPVLWAGPHCHLVHYPL
                     RGGKQYNLVVTFHSRQQEEWGVKDGSKEEVLSYFEGIHPRPRQMLDKPTSWRRWSTAD
                     REPVAKWGTKRITLVGDSAHPVAQYMAQGACMALEDAVTLGKALAQCEGDAAQAFALY
                     ESVRIPRTARIVWSTREMGRVYHAAGVERQVRNLLWKGKSQAEFYRGMEWLYGWKEDN
                     CLQPR"
     gene            45192..46142
                     /gene="dusC"
                     /locus_tag="N7B39_000899"
     CDS             45192..46142
                     /gene="dusC"
                     /locus_tag="N7B39_000899"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HGU2420924.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQECMAISGC
                     DSVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIGKLR"
BASE COUNT        11205 a        11503 c        12376 g        11324 t
ORIGIN      
        1 taagtactgt gagttatttg ttttggaaat gtttgttttt tcccggtagt gatttgtcaa
       61 aaaaggtgat taatcgatca cttaatggcg tcagcaacac ccagggcaaa ccgatcaata
      121 tcacggtcat cgaaccgaca atgatgtcag taaaccagtg tgcgccaatc attactctgg
      181 gaaacgcaaa aaccacaaaa ataataaggg cgataagacc tgcaactttg ccgaaataac
      241 gccacatgaa tgccgaaaaa ataagcagca tcatgccgtg atcgccggga aagctatctc
      301 gtgaggcatc tttcgtgggg acagagagca gttcgctgac gcggttaata tcggtaaaag
      361 tcaatgttgg gctggcccgt tttacaggaa ttaatgcctg acccagctgg tttaatacca
      421 ctgcagttaa tagcatgacc agaccaataa tcacgatacg tcgtctgcct ggggcgtttt
      481 ctttcagcca gaaactcagc atcaacattc ccattgccag cagtgaacaa ccgtcgaaag
      541 cgcgattgtt ggtcaatgca accagccaca aaaaggcctt actttcgacc agtttctgat
      601 taaagaaata aaaaatatcc gcatcaatcg gcaaccagaa tccatgatta acgggaatat
      661 accaggaaag aaacagcgcg aggccgacaa tattcaacaa cactatttgc ggcaaatttt
      721 taatcatatt ttctctaatt atcttactga aaaacgcagg caaccttacg cggtagtcgc
      781 taaacgaagc ttcaacagcg cgctctgcaa ggcattccag tcagcttcgc tgctggaaat
      841 cagctcaata cggctgtccg gtggcgcaac gttttgcgtt tcaatgtgca ggtcatcgcc
      901 ctgacggttg attcgcacca gcccttcggg aatacgcagc acgcctttga cgcgttccac
      961 cggtgcaagt cgcgcccatt ccagaatgcc aatggtgtcg aataccgtat cagcgtcgaa
     1021 tatccagccg caggcctgat gtccttgtcc actgttcaga ctgcgacgcc agcgttgatg
     1081 ctctggcagg cttaacgctg ctaacccttt tttcacgaca tgctgatgag aatgcgcggc
     1141 gctggcgggc aactcggcta aattgtgacg cggcaaatcc agaagatgac cggcaacttt
     1201 cccatgctca ctgtgaatta attgccgatc gccaccattt tgctgccacc agcgctgtag
     1261 cgcttgctcg ctttcaggtg tcgcacggtc ggatttattg gcgacaatga tgtctgcggc
     1321 agccagctgg tcacggaagt tttcattgct ggcgcttttt tcgtccagca gcaggcgtgg
     1381 atcgagaatg cacaacgtgg cgcgcagatc tatccacggt tcatagactg gtgcggttaa
     1441 cagatcgagg atctgtttcg gatggcccag cccagtcggc tctatcaaca agcggtctgg
     1501 ttttccctga cgcagtaagg tattcaaccc tacctgcatg ggtaaaccat taacgcagca
     1561 catgcagccg ccggggatct ctttcagcag tgcgccgcta tcggcgagca aagcaccatc
     1621 aattccgact tccccaaatt cattaaccag taccgcccat ttttcgttgg gatctttatg
     1681 ggctaacaga tgaagaatcg acgtggtttt cccgctgccg agaaaaccgg tgatgaggtt
     1741 ggtcctggtc acaacagctc cgcaatactc tggtaagtta actgagcgtt aatcctggct
     1801 aaaaacggca gaaaagagaa ggggagagcg gacgaaggcg agcttcatcc gctttaaccg
     1861 cagaaatcgt taaattttga gggtgtttaa tagcgcctga tgcgcgccga attgtgcgat
     1921 ttgttgccgg gcttgttcga tggcctgcac aaaatgcggg ttatctggca gatcatcacc
     1981 gaaaacttca cgcagggaaa gcagggcggt gacgcgttgc tcactgatgc tgcccgcaac
     2041 aagatcgcga attttatcgc taagcggatc gcgaacatca atggcatttc cggcatcatc
     2101 aacgccgctg acgtaacgca tccagcctgc aacgcctaat gccagcaacg accagtccgt
     2161 ttcgcgcccc agatgtatgc gaatacctgc cagcatgcgt tgcggtaatt tctggctgcc
     2221 atccatcgcg atttgccagg tcttatgttt cagcgccgga ttagcaaaac gtgcaattaa
     2281 cttatccgca tattgtgtta aatcgacatc tttaatccgc agtgtcggcg cttgctcatc
     2341 cagcattaat gttctggcgg catggcgaaa tgcgcgatcc tgcatgcaat cactgatatg
     2401 ggcgaatcct gagaggtaac ccagataagc gagaaaagag tggctgccat taagcatccg
     2461 cagtttcatc tcttcccatg gcaggacatc attcaccatt tgtacacctg cgacttccca
     2521 ggcaggacgc ccagcgacga agttatcttc caccacccac tggataaacg gttcgcagct
     2581 aatcgcgcag ggatcattta cccccagatg ctggctgatt tctgccagtg attcgtcggt
     2641 tgcagccgga acaatgcggt cgaccatggt tcccggaaaa ctgacgtgtt cttttatcca
     2701 cccggcgagt tctggcgaac gtttttctgc cattcccagc accgcgtttt tcaccacatg
     2761 gccattgtcg ggaatattgt cgcaggagag cacggtaaac ggtgtaaggc cgcgctcacg
     2821 gcggcgtttc agtgcttcga cgagaatacc cggtgcggag tgaggttctt caggggtttg
     2881 tagatcgtga ataatccgcg gattgctggt gtcgagtgca ccggtggccg ggtcaataca
     2941 atagcctttt tcggtaatcg tcagggaaac aattgccacc tgtggctcgc aaaatttctc
     3001 aataattgcc gctaaggaat ccagttttgc attcaggcat tcgtgaacgg caccgacaat
     3061 tatcgcctga ttgccgtccg cccctttctc cagcacggta tataaatggt tctgtgcgcg
     3121 gagctggctc atcagttgat caccgctgaa caagctgatt tcacagatcc cccagtcgcc
     3181 gccctgggca ttcagcacac gatcggtcag taacgcctga tgtgcgcggt gaaaggctcc
     3241 aaagccaaaa tgaacgatac gtgattgcaa ctgctggcga tcgtagcgtg gagcgtgtac
     3301 atgatgcggg agcgtaacgg aggcgattgt attcatagga gtcatgtctt agtaacgatt
     3361 aattaacaat gacagtgtaa agttatatga cagcaattga aattggtctg gattgttaat
     3421 gcgatgaagt gtgagggcga tcaatcaacg agtgaaaatt gagggcgtaa ggtgttcagg
     3481 aagcgcaaaa tagaagattg gtataataaa ttaaaaaagg gacagcctga gctatccctt
     3541 ttctgcatgc ggctgaagtt agtcagcacg ccccatataa cggcgttctt cgatatggat
     3601 acgaattttt tcgcccgggc ttaagtattc tggtacctga atcaccagac cagtgctcaa
     3661 tgtcgccggt ttgttacggg cgctggcaga tgcccctttg atgcctggtg cggtttcaac
     3721 gatttccaga tcaacggtct gcggcagctc aagcgccagc agttgaccgt cccaggtcag
     3781 cacctgcatg tccggcatgc cgccctctgg cataaacagc aactcttctt caatctgatc
     3841 tttggtgaag gtatacgggg tatagtcttc tttatccata aagacatatt cgttgccatc
     3901 gacataggag aagtcaacgt aacggcgggt cagcgtcacg gtgtcaacga tatcatctcc
     3961 tttgaaacgc tcttctactt tcagcccggt acggacatca gaaaaacgca ttttgtacag
     4021 cgtagcggca ccgcgggcag tgggcgactg aatatcaatg tccttaacca gcagcagttt
     4081 gccgttgtaa ttcagtacca tacctttttt aatttcgttc gctcttggca tcgaaaaatt
     4141 cctgtagtcg aaaatgtcaa aaatatcgcg acaaagtact cgcgacgagc ctttcgggca
     4201 agtggtattc gcacttttgc tggtgcaaaa aaggtggtac tgtgcgcgct catcaatccg
     4261 gtggttaacc ttaagagaac aacgtatgga atgccgtccg ggttgcgggg cgtgttgcac
     4321 tgccccttca atttccagcc ctattcccgg tatgccagat ggcaagcccg ccaatacgcc
     4381 ctgcattcag cttgatgaac agcaacgctg taaaattttc acctcgccac tgcggccgaa
     4441 agtctgtgca ggcttacaag ccagcgctga aatgtgcggt aactcgcgtc agcaggcaat
     4501 gacctggctt atcgatctgg aaatgctgac cgcgccttaa acatctttaa tccgcagtaa
     4561 gcaaaacaga gtggcgataa tcatcaccat cgcaaaccag aacacagcgt gataattcca
     4621 gatctcggcg acgatgcccg ccactgatcc cgcgatgatc cagcccacgc gcgacgtgtt
     4681 ggtatagagc gtggtggctg aacctgcctg accgggcatc agatcctgaa aatagagcat
     4741 cccgataccg cccagaatgc caataaaaat agcatttagc agctgcaagc ccaacagaat
     4801 gacaggagaa tgcgccatca gcattcctgc gtaaaaacag acgccaccca cggcagcaac
     4861 gcgcattaag aaacgcttac ccagacgttt ggcgaaatat ccggcaatca acatggtcgg
     4921 gatttccagc ccggcggcgg tccccatcat cacaccggcc agtttctcgg gaagatggag
     4981 ttcgttgata ataaatagcg gcatgttgat gatgtacagg ctgttcgagc cccacatcaa
     5041 tgtacaaatg acaaacagca gcagcgtatc gcgacggtta cggcgcggcg cttcaaccgt
     5101 gccggtcgcc agcggaagct ctttttgcat cgacggtaaa aacagccaca ccatcacgcc
     5161 gcaaacaata aatgctaccg ctgcgctcag atacattacc gtaaagctga aacccatcgc
     5221 taaggcataa gccagcggtg ggccaatgac ccatgccagt gaaacctgag cgcgtaaaaa
     5281 agagctgaac atcaccgcct cacgtccggt tttgtcggca tgttcacggg caagggcaaa
     5341 catttgcggg ttagcggtcg agccaaagct gctaagaaag acgccaacga atagcaaaac
     5401 aaagtagttg cgattccagg caaaaagggt gcaggccagc acgcctaaca ggcagcaaaa
     5461 gacaatcagc gatttgcgat cgccgcgctt atcagagcgc ccggcgagaa actgactcac
     5521 cagaacccca atgacagcgc tgccggtaaa gaagaatccc accatcgccg gacgggcatg
     5581 tacttcatcg gtaagaaaaa tactgagtgt cggggtttgc agagcgcccg caataccggt
     5641 gagaaaggca acgattaaaa acgccgtcga ggtcaggtca aacgatttcg cgctggagac
     5701 tgcgggggag ttatgcatgc tggtcgatca ctggaagagg aagacgcgga gtttacgctg
     5761 cttgtcagaa aataaacaga cagcgaaatc aaaagcgcca cgaataatca aaatgccatt
     5821 tcactgttgc tgattggctg ctgacgctgc tgaaatgaaa ttgctgataa cggattttcc
     5881 catcagcaat taggaaaaat gacaaaaaat tgtgcagcac atcaaacttt tgctcataac
     5941 tttacggctt tccttgcgtg ctgaaacaga agtattatgc tttcttgaaa cgtttcagcg
     6001 cgatcttgtc tttaacccta agccaggttg gcgctttttt tctcatagaa gctgaatcgt
     6061 ttcaattcag caagagagga gaactatgtt ccagttatcc gtacaggaca tccatccggg
     6121 cgaaaaggcc ggagacaaag aagaggcgat tcgccaggtc gctgcggcgc tggtgcaggc
     6181 cggtaatgta gcagaaggct acgtcaatgg catgctggcg cgcgaacagc aaacctcaac
     6241 gttcctcggc aatggtattg ctattccaca cggcactacc gacacccgcg atcaggtgct
     6301 gaaaaccggc gttcaggtat ttcagttccc ggaaggcgtc acctggggtg acggtcaggt
     6361 agcgtacgta gcgatcggta ttgctgccag ctcggatgag catctgggcc tgctacgcca
     6421 gctgacccac gtactgagcg atgattccgt tgctgaacaa ctgaagtcag caacaacagc
     6481 agaagaactt cgcgcattgc tgatgggcga aaagcagagt gagcagctga agctcgacaa
     6541 cgaaatgctg acgctggata tcgtcgccag cgatctgctg actcttcagg cgctgaacgc
     6601 tgcgcgtctg aaagaggcgg gggcagttga cgccactttc gtcaccaaag ccatcaatga
     6661 acaaccgctg aacctcggac agggtatctg gctgagcgat agcgccgaag gcaatctgcg
     6721 tagcgcaatt gcggtaagcc gtgcggcaaa tgcttttgat gtggacggcg aaacggcagc
     6781 catgctggtg agtgtggcga tgaatgacga tcagcccatt gcggttctta agcgtctcgc
     6841 tgatttgttg ctcgacaata aagctgaccg cttgctgaaa gcggatgcgg caacgttgct
     6901 ggcgctgctg accagcgatg atgcgccgac cgacgacgtg ttaagcgcgg agtttgtggt
     6961 gcgcaatgaa cacggcctgc atgctcgtcc aggtaccatg ctggtcaaca ccattaaaca
     7021 atttaacagt gatattaccg tgacaaacct tgatggcacc ggcaaaccgg caaacggacg
     7081 tagtctgatg aaagttgtgg cacttggcgt taagaaaggt catcgcctac gctttaccgc
     7141 ccagggtgca gatgctgaac aggcgctgaa agcaatcggc gacgctatcg ctgctggtct
     7201 tggggagggc gcataatgag cagacgtgtt gctactatca cccttaatcc ggcttatgac
     7261 cttgttggtt tctgcccgga aattgaacgc ggcgaagtga acctggtgaa aaccaccggt
     7321 ctgcatgcgg cgggtaaagg catcaacgtg gccaaagtat taaaagacct gggaattgat
     7381 gtcaccgttg gcggcttcct cggtaaagac aatcaggatg gttttcagca actgttcagc
     7441 gagctgggca ttgccaaccg tttccaggtt gtacaggggc gcacccgaat taacgttaag
     7501 ctgacggaaa aagacggcga agtgaccgac ttcaacttct cgggttttga agtcaccccc
     7561 gccgactggg aacgctttgt gactgattct ctgagctggc tcggtcagtt cgatatggtc
     7621 tgtgtcagcg gaagcttacc gtcaggcgtc agcccggaag cgttcaccga ctggatgact
     7681 cgcctgcgta gtcagtgccc ttgcattatc tttgatagta gccgtgaagc gttagtagca
     7741 ggtttgaaag cggcaccgtg gctggtgaaa cctaaccgcc gcgagctgga aatctgggca
     7801 ggccgtaaac tgcctgaaat gaaagatgtg attgaagctg cgcatgcgct gcgtgaacaa
     7861 ggtattgcgc atgtcgttat ttcactgggt gccgaaggcg cgctttgggt taatgcctcc
     7921 ggcgaatgga tcgccaaacc accgtcagtc gatgtcgtaa gcaccgttgg cgcaggggat
     7981 tctatggttg gtggcctgat ttatggcttg ctaatgcgtg aatccagtga acacacactg
     8041 cgtctggcga ctgctgttgc agccctggcg gtaagtcaaa gcaatgtggg tattaccgat
     8101 cgtccgcagt tggccgcaat gatggcgcgc gtcgacttac aaccttttaa ctgacagcag
     8161 gagaggcata atgaaaacgc tgctgattat tgacgctaat ctcggtcagg cacgcgccta
     8221 tatggcgaag accctgctgg gcgcggcggc gcgaaaagca aaactggaaa tcatcgacaa
     8281 tccgaacgac gcagaaatgg cgattgttct cggtgattcc atcccgaatg acagcgcgct
     8341 gaacggtaaa aatgtctggc tgggcgatat ttcccgggca gttgcgcacc ctgagctgtt
     8401 cctgagtgaa gccaaaggcc atgcgaaacc ttacactgcg ccggtcgctg cgacagcacc
     8461 ggttgccgcc agcggtccga aacgcgtagt tgcggtgact gcttgcccga ctggcgtagc
     8521 acacaccttt atggcggctg aagccattga aaccgaagcg aaaaaacgtg gctggtgggt
     8581 gaaagttgaa acccgtggtt ctgttggcgc gggtaatgca atcactcccg aagaagtagc
     8641 cgcagcggat ctggtgattg tggcggcaga tatcgaagtg gatctggcga aatttgctgg
     8701 taaaccgatg tatcgcacct ctaccggtct ggcgctgaag aaaaccgcgc aggaactgga
     8761 taaagcggtt gctgaagcaa cgccgtatga accggcgggc aaagctcaaa cggcgacctc
     8821 tgaaggtaag aacgagagtg caggcgcata ccgtcacttg ctgacgggcg tctcttacat
     8881 gctgccgatg gtcgttgcag gtggtctgtg tatcgcgctt tcttttgctt ttggtatcga
     8941 agcgtttaaa gagccgggta cgttggcagc ggcgctgatg cagattggtg gtggttcagc
     9001 ctttgcgctg atggtgccgg tactggcagg ttatattgcc ttctccattg ccgatcgtcc
     9061 gggtctcacg ccgggtctga ttggcggtat gctggcggtc agcaccggtt ctggcttcat
     9121 tggcggtatt attgcgggct tcctggctgg ttacattgcg aagttaatca gtacgcaact
     9181 gaaactgcca cagagtatgg aggcgctgaa accgatcctg atcattccgc taatttccag
     9241 tctggtggtc ggtctggcga tgatctacct gatcggtaaa ccggttgctg gcattctcga
     9301 agggttgact cactggctgc agaccatggg gactgcgaat gcggttctgc tgggggcgat
     9361 cctcggtggc atgatgtgta ctgacatggg cggtccggta aataaagcag cgtacgcatt
     9421 cggtgtgggt ctgctgagta ctcaaaccta tggcccgatg gcggcgatta tggcggcagg
     9481 tatggtgcca ccgctggcaa tgggtctggc aacaatggtg gcgcgtcgca aatttgacaa
     9541 agcgcagcag gaaggtggca aagccgctct ggttcttggt ctgtgcttta tttcggaagg
     9601 tgcaattcct tttgctgctc gtgatccgat gcgtgtgctg ccgtgttgta tcgttggtgg
     9661 ggcgctgact ggcgcaatct caatggcgat tggtgcgaaa ctgatggcac cgcacggtgg
     9721 tctgtttgtt ctgctgatcc ctggcgctat tacgccggta ctgggttacc tggtagcaat
     9781 tattgccggt acgctggtgg cgggtttggc ctatgccttc ctgaaacgtc cggaagtgga
     9841 cgcagtagcg aaagccgcgt aataaaaggt gtgttacagg gcagaaattt actgccccgt
     9901 atcattggat gagcgcaaag agaatgaccg gtttgtgaat gtaattttat gaatttaaat
     9961 ggttttatct ttcagtagaa acctgaaacg ttcatcaaac actaaggtta tttcctcaag
    10021 ggcagattct tttctgccct tttttagtgt tcatgttctg tgcatcccgt cacaaattcc
    10081 tcgcttttgt aattcaggca tttaatcgca ttttgtgagc ctgctcaaat ctgcccgctt
    10141 tgcctctctg gcggcaattt actttacgta aaacatgcat actatgagca catgtttaaa
    10201 tatgaacaca tgtttaaacc gtctttgcag tatctatatc cttgtggatt gacagggttg
    10261 gtaatttttt accagaggac gtgctatgcg cgaaaaggat tatgtcgtaa ttataggctc
    10321 ggcgaatatt gatgtcgccg gatattcaca tgaatcatta aattatgcgg attcaaatcc
    10381 aggtaaaata aaatttacgc ctggtggggt agggcgcaat attgcacaaa acctggcgtt
    10441 gctgggtaat aaagcctggc tactgagcgc cgtaggcagt gatttttatg gccaatcgct
    10501 gctaacgcaa accaatcaat ctggcgttta tgtcgataaa tgcctgattg tgccgggaga
    10561 aaatacgtcg agctatttat cattactcga taataccggt gaaatgctgg ttgctataaa
    10621 tgacatgaat attagcaacg ctattacagc tgaatatctc gcacagcacc gtgaatttat
    10681 tcagagggca aaggtcattg tcgcggactg taatatcagt gaagaggcac tggcatggat
    10741 tctggataat gctgccaacg tacccgtatt tgtcgatccg gtttccgcgt ggaaatgcgt
    10801 caaagtacgc gagcgattaa gtcaaatcca tactctcaag ccaaaccgcc ttgaagcgga
    10861 aaccctgagt gggattgcgc tgtcagggcg tgaagatgtg gcaaaagttg ctgcctggtt
    10921 ccatcaacat ggcctgaacc gactggtatt gagcatgggc ggcgacggcg tttattacag
    10981 cgatatcagc ggtgaaagtg gctggtctgc gccgatcaaa accaatgtta ttaatgttac
    11041 cggagcgggc gatgccatga tggcgggact tgcttcgtgt tgggtagacg gaatgccgtt
    11101 tgccgaatct gttcgtttcg cacagggatg ttcgtcaatg gcgctctcct gtgaatacac
    11161 caataacccc gatttatcga ttgccaacgt tatatcgtta gtggagaacg cagaatgtct
    11221 gaattaaaaa tttcccctga attattacaa atttccccgg aagtgcagga cgctttaaaa
    11281 aacaaaaaac cggttgtggc gctggaatcg accattattt ctcacgggat gccgttccca
    11341 caaaatgccc agaccgcaat tgaagtagaa gaaactattc gtaaacaggg cgcagtacct
    11401 gccactatcg ccattattgg cggcgtgatg aaagtgggtt taagcaaaga agaaattgaa
    11461 ttactgggtc gtgaagggca taacgtgact aaagttagtc gtcgcgattt accttttgtc
    11521 gttgccgcag gaaaaaatgg tgcaaccacc gtggcttcaa cgatgattat tgcggcgctt
    11581 gccggaatta aagtatttgc caccggcgga attggtggtg ttcatcgagg ggcggaacat
    11641 accttcgata tttctgccga tttgcaagaa ctggcaaata ctaatgtcac cgttgtttgt
    11701 gccggggcga aatctattct cgatttagga ttaaccactg agtatttaga aaccttcggt
    11761 gtgccgttaa ttggctatca gactaaagcg ctgcctgcgt ttttctgccg taccagcccg
    11821 tttgacgtca gcattcgtct cgacagcgcc agtgaaattg cccgtgcaat ggcggtgaaa
    11881 tggcaaagcg ggctgaacgg tggcctcgtg gtagcgaacc cgatcccgga acagtttgcg
    11941 atgccagaac acactatcaa tgcggtgatc gatcaggcgg tagctgaagc tgaagcgcag
    12001 ggtgttattg gtaaagaaag tacgccattc ctgctggcgc gcgttgctga gctgaccggc
    12061 ggtgacagcc tgaaatccaa catccagctt gtgttcaaca acgccattct ggcgagcgaa
    12121 attgccaaag aatatcagcg tctcgcgggt taatttctga cgggcagaaa gccgtcgccc
    12181 gtactcatca aacataccga aacctggcgc tgacgccggg tttactggca tgaagggaaa
    12241 atcattatgg acataatgag aagtgttgtg gggatggtgg tgttactggc aatagcattt
    12301 ctgttgtcag tgaataaaaa gagcatcagt ttgcgtacgg ttgcagccgc actactgctg
    12361 caaattgctc ttggtggcat catgctctat tttccgccgg gaaagtgggc tgtagaacag
    12421 gcggcattag gcgttcataa agtgatgtct tacagtgatg ctggtagcgc cttcattttt
    12481 ggttcgctgg tcgggccgaa aatggatgtc ctctttgacg gtgcgggttt tatcttcgcc
    12541 tttcgcgtac ttccggcgat tatcttcgtc actgcgctca ttagtctgct gtactacatt
    12601 ggcgtgatgg ggctgctgat tcgcatcctt ggcagcattt tccagaaagc cctcaacatc
    12661 agcaaaatcg aatcttttgt cgcagtcacc actattttcc tcgggcaaaa tgagatcccg
    12721 gcgatcgtta aaccgtttat cgatcgcatg aatcgaaacg agttgtttac ggctatttgt
    12781 agcgggatgg cgtccattgc tggttcgatg atgattggtt atgccggaat gagcgtacca
    12841 attgactacc tgttggcggc atcgctgatg gcgatccccg gcggtatttt gtttgcacgt
    12901 attcttagtc cagcaaccga gctttcgcag gtcacgtttg aaaatctgtc gttcagcgaa
    12961 acgccgccaa aaagctttat cgaagcagcg gcgagcggtg cgatgaccgg gctgaaaatc
    13021 gccgctggtg tggcgacggt ggtaatggca tttgtggcaa ttattgcgct gatcaacggc
    13081 attatcggcg gaattggcgg ctggtttggc ttcgccaatg cttctctgga aagtattttt
    13141 ggctatgtgc tggcaccgct ggcgtggatc atgggtgtgg actggagtga tgccaacctt
    13201 gcgggtagcc tgattgggca gaaactggcg attaacgaat tcgtcgctta cctgagtttc
    13261 tccccatacc tgcaaacggg cggcacgctg gaagtgaaaa ccattgcgat tatctccttt
    13321 gcgctttgtg gttttgctaa ctttggttct atcggtgttg tcgttggggc attttcggct
    13381 atttcgccaa aacgcgcacc ggaaatcgct cagcttggtt tacgggcgct ggcagcagca
    13441 acgctttcca acctgatgag cgcgaccatt gccgggttct ttattggtct ggcgtaatgc
    13501 tcaatttgta gaccggataa aacgcatcaa gcgttgcatc cggcaatcgc gctattgcaa
    13561 ataattttta ctgcatcatc ccggagaatt tattctccgg gtccatctcc agtgccagcc
    13621 ctgacaactg ctttctgttt ttaattagat atcctttctt gcttttaatt aataaaccgt
    13681 cgtgaatgaa ttgcgcgaga acatataaaa gatgtcgata agaaacgcct aaatactctg
    13741 ccgcttgcgt atgcttttcg tgataaagat caccttcttg tgagagtaat ataaatgctg
    13801 ccaggcgatt cactaacgga aatgattgat tctgagttaa agaaacaata ttacgataat
    13861 ttttatgact taaggtgacg caaagttttc gtaaaaatag ggtgtcgttt aataacagcg
    13921 gacggtaatg tttcataggg agcgcaaggc accaacactc ttcaatagcc tgtaccgcac
    13981 gcggttcatg gtctttatcg attaactcaa tctcaccaat aaaacagggg gcggcaaaga
    14041 aatcgatcag cgacacgcga ccattagcta gcgtggcgta aagcctggcg cggcctcgcg
    14101 tcaggtaaaa cagccaggac ggctgttgcc cttcctgcac aatgtagtca cgcgctaaaa
    14161 aatgaaacag ccgcgtatcg gctgaaacat ccgttaaaaa gcaatcctta aacgcggatt
    14221 cactcatcaa ttgctgctta agatcattat tgtggatttc tttcacgatg tttatccgct
    14281 atgagatttc tcatatttag catacgtacg ctgttgttaa gattcaatca tcggcccgat
    14341 cgaaatttct ttgctgtaga aaaaataacg caactggaaa cagaggaaat aaacaatgga
    14401 aaagagaaaa attattctgg attgtgatcc gggtcatgat gatgctattg ctataatgat
    14461 ggcggcgaaa catccggcaa tagatttatt aggcatcact attgtagcgg gtaatcagac
    14521 gcttgataaa acattaatta atggcctgaa tgtttgccag aaactggaga ttaatgttcc
    14581 ggtttatgcg gggatgccac aacccattat gcggcaacaa atcgttgccg ataatattca
    14641 cggtgatacc ggactggatg gcccggtatt cgagccgctg acccgccagg cagaaagcac
    14701 tcatgcggtg aaatacatca ttgataccct gatggcaagc gatggcgata tcactctggt
    14761 gccggttggt ccgctttcaa atatcgcggt ggcaatgcgt atgcaacccg cgatcctgcc
    14821 caaaatccgt gaaattgtgc taatgggcgg cgcttacggt acaggcaact tcacgccatc
    14881 tgccgagttc aacatctttg ctgacccgga agccgcacgc gtggtgttca cctctggcgt
    14941 tccattagtg atgatgggcc tcgatctcac caaccagacc gtttgcaccc cggacgtgat
    15001 tgcacgaatg gaaagggcag gcggccctgc cggagagctg ttcagcgaca tcatgaactt
    15061 cactctcaaa acccagttcg aaaactacgg cctggctggc ggcccggtgc acgacgccac
    15121 ctgcatcggt tatctgatta accctgatgg cattaaaacc caggagatgt acgtcgaagt
    15181 ggacgtcaac agtggccctt gttatgggcg taccgtctgc gacgagctgg gcgttcttgg
    15241 caagcccgct aataccaaag tcggcatcac tattgatagc gactggttct ggggcttagt
    15301 cgaagagtgc gtgcgcggct acatcaaaac ccattaagtg ttgataacaa gccgggccgg
    15361 tatcgcaaga tgcaggcctg gctatttctg atgggcagaa agccgtcgcc caatatgaca
    15421 tgaagagata atcactatgg atgtcatgag aagtgttctg ggaatggtgg tattgctgac
    15481 gattgcgttt ttactgtcag taaacaagaa gaagatcagc ctgcgtaccg ttggcgcggc
    15541 gttagtgtta caggtcgtga ttggcggcat tatgctttgg ttaccgccag ggcgttgggt
    15601 cgctgaaaaa gtcgcttttg gtgtgcataa agtgatggcg tacagcgacg cgggtagcgc
    15661 atttatcttc ggttctctgg tcggaccgaa aatggacacc ttatttgatg gcgcaggatt
    15721 tatctttggt ttcagggtat taccggcaat tatcttcgtc actgcactgg tgagtattct
    15781 ctactacatc ggtgtgatgg ggattttaat tcgcattctc ggcggtatat tccagaaagc
    15841 attaaatatc agcaagattg agtcattcgt cgcggtcact accattttcc tcgggcaaaa
    15901 cgaaattccg gcgattgtga agccctttat cgatcggctg aatcgcaatg aattatttac
    15961 agcgatttgt agcggcatgg cctcgattgc tggttcgaca atgattggtt atgccgcact
    16021 gggcgtgcct gtggaatatt tgctggcggc atcgttaatg gcgatccccg gggggatctt
    16081 gtttgcccgc ctgctaagcc cggctacgga atcttcgcag gtttctttta ataacctctc
    16141 tttcaccgaa acaccgccaa aaagcattat tgaagccgcc gcgacagggg caatgaccgg
    16201 gctgaaaatc gccgcaggtg tggcgacagt agtgatggca ttcgtcgcca tcattgcgtt
    16261 aattaacggt attatcggcg gcgttggcgg ctggtttggt tttgaacatg cctcgctgga
    16321 gtccattgta ggttatctgc tggccccact ggcgtgggta atgggtgttg actggagtga
    16381 tgcgaatctt gccgggagtt tgattggaca gaaactggca ataaatgaat ttgtcgctta
    16441 tctcaatttc tcaccctatc tgcaaacggc tggcactctg gatgctaaaa ccgtggcgat
    16501 tatttccttc gcgttgtgcg gtttcgctaa ctttggttct atcggggtgg tggtgggggc
    16561 gttttctgcg gttgcgccac accgtgcgcc ggaaatcgcc cagcttggtt tacgcgcgct
    16621 ggcggcggcg acactttcta acctgatgag tgccaccatt gccgggttct ttattggttt
    16681 agcgtagtgt gcgatttatg agccggataa gatgcggtca agcgtcgaat ccggcaacat
    16741 tgttacacca ttggcactaa tgaaagcgcg ttatcggcag acagggtagg gttgtttaag
    16801 ctgccgctgg cgcgtgaaat tgccgcgcag gccatcgcaa aacgggcgct gtcacggaaa
    16861 ttgtttcctt ccagaaagct atataccagg cccgccataa aaccatcgtc agcaccaaaa
    16921 ctgtctactg tcgtatgcgc tggtgcggtc agcaaaaatt gttctccatc cttttcgctg
    16981 caataaactg actcatcggg caaataaaca aacagttgct gaacaccttg ctgatgcaac
    17041 gcattcactg cagcattacg gtcagcatcg ctggtgatcg cctgtcccca taaaatttcc
    17101 agctccggta aagtgggttt cagggtgtga atatgcgcca gccagtgttt gattttgccc
    17161 gctttgaatt ctgaaacggt atcgacaaac accgggatct caccagcgag ggtaaagacc
    17221 cattccagcg cctcggctgt caggttgcaa tcagccagta ccacgcccgc atgacgaagt
    17281 aaatcgcgcg acccgttcaa taattgcggc gataactgtt ccagcagatg ggtatcgtta
    17341 atcgccagca cggtttcatc gtctcgattg gcaattgcca gatacgtcga tgtgctttga
    17401 ccatgtaaac gaacgcagcc ggagacattc acgccggcgc ggcgcgtttc ttccaggagc
    17461 atttcgccat aaaagtcatc gccaatcact gaaagcaaat ggacgtcacg gcctaacagc
    17521 gccagattgt gggcgatgtt gcgtcccacg ccgcccgctg agcaatgaat tgtaccggga
    17581 tgagaagccg attgcgggta acggatatcc gccatcccgc gaatatccat attgattgtc
    17641 cccaccacta cgcagtattc ctgctcggtg agaatgtaac ctttgccttt aatccggcct
    17701 ttgcgcatta aatccataat atgcgccgca acgcgcgaac ggctgatttg cagcatgtcc
    17761 gcaatttcgt tctgctgaat cagcgggtta cgccgtaaaa ttgcaaggat ctccttttcc
    17821 cggttattca tcttcaggct accgcttttt cagtttgttg cgctttcagc caggcgatct
    17881 cttctgccca gatatccggg ttgatggttt cgaggatcag cggaatgccg tcgaaacggt
    17941 cgtcctgcat gatccagcgg aacgcatcat gaccgatatt gccttcaccg aggctatgat
    18001 ggcggtcaac gcggctgcca aaggtgcttt tcgcatcgtt aaggtgcatc ccgcgcagat
    18061 acttaaagcc gacagtacgg gcaaaatccg cgaatgtttt ctcgcattcg gctggagtac
    18121 gcaaatcata cccggcagcg aaagcatggc aggtatcaat gcagacgccg acgcgggatt
    18181 tatcttccac gccgtcgata atcgccgcga gatgttcgaa tttaaaccct aagttactgc
    18241 cctgaccggc ggtgttttct atcaccgctg tcacaccttg agttttatcc agcgcaatgt
    18301 tgatggattc ggcaatacgc gcaaggcaat cctcttctga aatctgcatc agatggctgc
    18361 cagggtggaa gttgagcaaa gaaagcccca gctgttcgca acgctgcatt tcatctataa
    18421 aggcatcgcg cgatttttcc agagcttcag tgaccggatg tccgaggtta atcagataac
    18481 tgtcgtgggg aagaatttgc gccgatgtgt agtgatattt ttcacaggcg gctttgaatt
    18541 catcgatggt ttgcgtcgtg agcggtgcgg cacgccactg acgttggttt ttggtgaaca
    18601 aggcaaacgc ggttgcgtcg atttcggcgg cgcgaattgc ggcatttgcc agaccgccag
    18661 cagcactaac gtgcgctcca atgtatttca tgcgaggact cctgttaaac ccgctggagg
    18721 aaaacggtaa tgatagcggg ttaacaggag caagatgtag tggtttatgc gatgacgctt
    18781 tgaatcacat agttaatcgc accaccacca acaatcagcc aggcaaacag taccagtgcc
    18841 atcagcagag gtttcgcccc agcttttttc agcgcgctga cgtgagtggt cagacccagc
    18901 gccgccatcg ccattgccag caggaaggta tccagcgtta ccagcatgtt caccacgctc
    18961 tgcggtaaca ggtggaacga gttaaagatg gcaactacga tgaacaagat ggcaaaccac
    19021 ggaatagtga ttttgctttt ctcgccgctg ttcgccccag acagctgttt aacacgcgcc
    19081 gccagcagga tgaggaacgg agccagcatc atcacgcgca gcattttgga aataactgct
    19141 gcgttttccg catccgggct gatggcatga cctgccgcca ccacctgcgc cacttcgtgc
    19201 acagtagaac caatgtagat accgaaagtt tccggactaa accattgaga catcagcgga
    19261 tatatcgccg ggtagaggaa aatcgcgacg gtcccgaaga taacaacggt tgcaacagcc
    19321 acggttactt tactggcttc cgctttcact accggctcag tcgccagtac cgcggcagca
    19381 ccacagatac tgctaccggc accgatcaac cagctggtgt gcttatccag accaaacact
    19441 ttctgcccca ggaagcaagc cagcaggaag gtactggaca gcgtcagcac gtcaatgatg
    19501 atcccactga taccgacatc ggcaatttgc gagaacgtca gacggaagcc ataaagaatg
    19561 atacccagac gtaataaata ttgcttggca aacagcacac caccgtcaca gcttttccag
    19621 atgtgcggat agatggtgtt gcctaaaacc atccccaaca agattgcgag ggtgagggca
    19681 ctaaacccgg cacccgcaac cgcgggaatg gaaccacccc acagggcgac cccggtgata
    19741 actgcactca gggctaaccc cggaataaaa tgccacagtg tacgatgttg tttctgtaag
    19801 gtgatattcg tcataaccct ctcctttaac cgggcataag gttacgacta actggtttaa
    19861 aaataaaatt gattatatat ttataattaa tctttataag tggtaagcga ctatgcacat
    19921 caccctccgg cagttggaag tttttgcaga agtattgaaa agtggatcaa ccacccaagc
    19981 gtcggtgatg ctggcgttgt cgcaatcagc agtgagcgca gccttgaccg acctggaagg
    20041 gcagcttggc gtgcaactgt ttgatcgcgt ggggaaaaga ctggttgtta atgaacacgg
    20101 gcggctgctc tatccgcgtg cgttggcatt gcttgaacag gcggttgaaa tcgaacaact
    20161 gtttcgcgaa gacaacggcg cgattcgtat ctatgccagt agtaccatcg gtaactacat
    20221 tctgcctgca gttatcgccc gttatcgcca tgattatccg cagttgccga ttgaacttag
    20281 cgttgggaat agccaggacg tgatgcaagc ggtgctggat ttccgcgttg atattggctt
    20341 tattgaagga ccgtgccaca gcactgaaat catttctgaa ccgtggctgg aagacgagct
    20401 ggtggttttc gccgcgccga cttcgccgtt ggcccgtggt ccggtcacct tagaacagct
    20461 ggccgctgcg ccgtggatcc tgcgtgaacg cggttccggc acgcgggaga ttgtcgatta
    20521 tctgttgctg tcacatttac cgaagtttga gatggcgatg gaattaggta actccgaggc
    20581 aatcaaacat gcggtgcgtc atgggttggg aattagttgc ctgtcgcgac gtgtgattga
    20641 agatcaattg caggcaggca cattaagtga agttgcggtc cctctgccgc gcctgatgcg
    20701 tacgttgtgg cgtatacatc atcggcaaaa acacctttcc aacgcgctac ggcgctttct
    20761 ggactattgc gatcccgcaa atgtgccgcg ttaagttgct gtacaagaac atgctggtgc
    20821 tgtgtcgatt tcgtgacgca gcgccttcag catgcattcg ccagaaaaga gattggctgc
    20881 tttacttata atccctgggc gatcatgaag gtgtcttata accgtgtatt tctgccggaa
    20941 ggattgccaa tcgtctgcta caatcgcgcc tcatttttta gatggatagc atttttgtat
    21001 ggtttccgaa actaaaacca cagaagcgcc gggcttacgc cgtgaattaa aggcgcgtca
    21061 cctgacgatg attgccattg gcggttccat cggtacaggt ctttttgttg cctctggcgc
    21121 aacgatttct caggcaggtc cgggcggggc attgctctcg tatatgttga ttggcctgat
    21181 ggtttacttc ctgatgacca gtctcggtga actggctgca tatatgccgg tttccggttc
    21241 gtttgccact tacggtcaga actatgttga agagggcttt ggcttcgcgc tgggctggaa
    21301 ctactggtac aactgggcgg tgactattgc cgttgacctg gttgcagctc agctggtcat
    21361 gagctggtgg ttcccggata caccgggctg gatctggagt gcgttgttcc tcggcgttat
    21421 cttcctgctg aactacatct ctgttcgcgg ctttggtgaa gcggaatact ggttctcact
    21481 gatcaaagtc acgacagtta ttgtctttat catcgttggc gtgctgatga ttatcggtat
    21541 cttcaaaggc gcgcagcctg cgggctggag caactggaca atcggcgaag cgccgtttgc
    21601 tggtggtttt gcggcgatga tcggcgtagc tatgattgtc ggcttctctt tccagggaac
    21661 cgagctgatc ggtattgctg caggcgagtc cgaagatccg gggaaaaaca ttccacgcgc
    21721 ggtacgtcag gtgttctggc gaatcctgtt gttctatgtg ttcgcgatcc tgattatcag
    21781 cctgattatt ccgtacaccg atccgagcct gctgcgtaac gatgttaaag acatcagcgt
    21841 tagtccgttc accctggtgt tccagcacgc gggtctgctc tctgctgcgg cggtgatgaa
    21901 cgcagttatt ctgacggcgg tgctgtcagc gggtaactcc ggtatgtatg cgtctactcg
    21961 tatgctgtac accctggcgt gtgacggtaa agcgccgcgc attttcgcta aactgtcgcg
    22021 tggtggcgtg ccgcgtaatg cgctgtatgc gacgacggtg attgccggtc tgtgcttcct
    22081 gacctccatg tttggcaacc agacggtata cctgtggctg ctgaatacct ccgggatgac
    22141 cggttttatc gcctggctgg ggattgccat tagccactat cgcttccgtc gcggttacgt
    22201 attgcaggga cacgacatta acgatctgcc gtaccgttca ggtttcttcc cattggggcc
    22261 gatcttcgca ttcattctgt gtctgattat cactttgggc cagaactacg aagcgttcct
    22321 gaaagatacc attgactggg gcggcgtagc ggcaacgtat attggtatcc cgctgttcct
    22381 gattatttgg ttcggctaca agctgattaa aggaacccac ttcgtacgct acagcgaaat
    22441 gaagttcccg cagaacgata agaaataagt ttctcccttc cttgctaagc cctctcaacc
    22501 gagagggctt tttcaattcc atttccctga caaatcatgc ggatataaaa tttaacattt
    22561 ggattgataa ttgttatcgt ttgcattatc gttacgccgc aatcaaaaaa ggctgacaaa
    22621 tcagaggctg gtccggcttt ctgggatgat cacctgcata caaataagtc caccgcgatg
    22681 ctgccgtacg caaggggacg tgaagaagat gtgagcgata acccatttta ttttcgtagt
    22741 tacctcatgg agatatggaa tgtttaggtt gaaccctttc gtacgggtcg ggctgtgttt
    22801 gtccgctatt tcttgtgcat ggcctgtgtt agcggtcgat gacgatggcg aaacgatggt
    22861 tgtcactgca tcttccgttg aacaaaacct taaagatgca cccgccagta tcagcgtcat
    22921 tacccaggaa gacctgcagc gaaaaccggt acagaatctg aaggatgtcc tcaaagaagt
    22981 gcctggcgta caactgacga acgaagggga taaccgtaag ggcgttagta ttcgtggtct
    23041 ggacagcagc tacaccctga ttcttgtcga cggtaaacgc gttaactccc gcaatgccgt
    23101 cttccgccac aatgatttcg atctgaactg gatcccggtc gattccatcg aacgtattga
    23161 agtggtccgt ggcccgatgt cgtcgctgta cggttccgat gcgctcggcg gtgtagtgaa
    23221 tatcatcacc aaaaaaatcg gtcagaaatg gtcgggtacc gttaccgtcg ataccaccat
    23281 tcaggaacat cgcgatcgcg gtgataccta taacggtcag ttctttacca gtggaccatt
    23341 aattgatggt gtgctgggaa tgaaagctta cggcagcctg gcaaaacgtg aaaaggatga
    23401 cccgcaaaac tcaacgacca ccgataccgg agaaacgccg cgtattgaag gattctccag
    23461 ccgcgacggc aatgtcgaat ttgcctggac accgaatcaa aatcacgatt ttactgccgg
    23521 atacggtttc gaccgtcagg atcgtgattc cgactcgctg gacaaaaacc gcctggaacg
    23581 ccagaactac tccgtcagcc ataatgggcg ttgggattac ggcaccagcg aactgaaata
    23641 ctacggtgag aaagtcgaga acaaaaaccc tggcaacagc agcccgataa cttccgaaag
    23701 caatacggtc gacggcaaat acacgttgcc gctgacggcg attaatcagt ttctcacggt
    23761 tggcggtgaa tggcgtcacg acaaacttag cgatgcggtg aacctgaccg ggggaaccag
    23821 ctccaaaacg tctgccagcc agtacgcgct gtttgtggaa gatgaatggc ggatcttcga
    23881 gccgctggcg ctgacgaccg gcgtgcgtat ggacgatcac gaaacctacg gtgaacactg
    23941 gagtccgcgt gcctacctgg tttataacgc caccgacacc gtaacggtga aagggggctg
    24001 ggcgacggca tttaaagcac cttctctgtt gcaacttagc cctgactgga cgagcaattc
    24061 ctgccgtggc gcatgtaaga ttgtgggtag cccggatctg aaaccagaaa ccagcgaaag
    24121 ttgggagctg gggctttact acatgggtga agaaggctgg ctggaagggg ttgaatccag
    24181 cgttaccgtt ttccgtaacg atgtgaaaga tcgtatcagc atcagccgta cgtctgacgt
    24241 caatgctgca ccgggctacc aaaactttgt tggttttaca gcgaacggtg tgccggtatt
    24301 tagctactac aacgttaaca aagcacgtat tcagggcgtg gaaaccgagc tgaaaattcc
    24361 gttcaacgat gaatggaaac tgtcactcaa ctacacctac aacgatggtc gtgatgtcag
    24421 caacggcgaa aacaaaccgc tatccgatct gccgttccat accgctaacg gtacgctgga
    24481 ctggaaaccg ctggcgctgg aagactggtc attctatgtt tctggtcact ataccgggca
    24541 gaaacgagcc gacagcgcga cggctaaaac accgggcggt tataccatct ggaataccgg
    24601 cgcggcctgg caggtgacta aagacgtcaa actgcgcgca ggcgtgctga accttggcga
    24661 caaggatctc agtcgtgacg actacagcta taacgaagac ggacgtcgtt actttatggc
    24721 agtggattat cgcttctgat gagaagatgc ccggcgaacc gggcggactt tcacttcagt
    24781 aaatactgcg catggaagcg caggtgatcc tctataaaag aggcgatgaa gtagtaactg
    24841 tgatcatatc ccggctgaat acgcagcgtc atcggccatg ctttctggcg cgcggcttct
    24901 gcaagtactg caggttgcag ttggtcagca agaaactgat cgttatcacc ctgatcgata
    24961 agcgtcggga tcgcatcctg cgcgttactg gcatacatca gcgcgcaact gtcccattcc
    25021 agccatgcat ttttgtcctc acctaaatag cggctaaacg ctttgattcc ccacgggacg
    25081 ctgcacggat tcacaattgg cgcaaaggcc gaaacgctgg tgtatttgcc cgggtttttc
    25141 agcgccataa tcagcgcacc gtgaccaccc attgaatgac cgctaatagc gcagcggtcg
    25201 ctgacattaa attgcgactg aaccagcgcc ggtaattcat cacgcagata atcatacatc
    25261 cggtaatgcg tcgcccacgg cggttgcgtg gcattaagat aaaagcctgc gccctggcct
    25321 aaatcgtagc catcgtcgtt ggcaaccttt tcgccgcgcg ggctggtgtc tggcataacc
    25381 agtacaatcc ccagttccgc cgccacgcgc tgggcacccg ccttggtggt gaagttctcg
    25441 tcattgcagg ttaatccgga aagccagtac agcactggtg gcggggtgtg atcacgaggg
    25501 ggtgggagaa agatactgaa cgtcatcggg cagtttaagg tactggagtc gtgtcgccag
    25561 cgttgctgcc agccttcaaa acagcggtgc tcttcgagca tttccatgcg tggctccttg
    25621 ttgtgttgtt tgcaatttct tcatcatacc gtttattcaa tgtgctgtga gtaactttca
    25681 cttccgtatt tgcataacga tgttttaaca tctgctgatg aaaggcagcg gcaattacaa
    25741 taattatcgc tgtgaatact ggattatgtg cgccgcctca cgcacaataa tcaggctgta
    25801 aatcagctta ataactttgc ccccacgcag ggcggaggcg tcacacctgc aggagaaatc
    25861 ataaatgcca tcactcagta aagaagcggc cctggttcat gaagcgttag ttgcgcgagg
    25921 actggaaaca ccgctgcgcc cgcccgtgca tgaaatggat aacgaaacgc gcaaaagcct
    25981 tattgctggt catatgaccg aaatcatgca gctgctgaat ctcgacctgg ctgatgacag
    26041 tttgatggaa acgccgcatc gcatcgctaa aatgtatgtc gatgaaattt tctccggtct
    26101 ggattacgcc aacttcccga aaatcaccct cattgaaaac aaaatgaagg tcgatgaaat
    26161 ggtcaccgtg cgcgatatca ctctgaccag tacctgtgaa caccattttg ttaccatcga
    26221 tggcaaagcg acggtggctt atatcccgaa agattcggtg atcggtctgt caaaaattaa
    26281 ccgtattgtg cagttctttg cccagcgtcc gcaggtgcag gaacgtctga cgcagcaaat
    26341 tctcattgcg ctacaaacgc tgctgggcac caataacgtg gctgtctcta tcgacgcggt
    26401 gcattattgc gtgaaggcgc gtggcatccg cgatgcaacc agtgccacga caacgacctc
    26461 tcttggtgga ttgttcaaat ccagtcagaa tacgcgccac gagtttctgc gcgctgtgcg
    26521 tcatcacaac tgatttaaag gcaggaacca tggagcgcaa cgtcacgctc gactttgttc
    26581 gcggcgtcgc cattctgggg atcctgctat taaacatcag cgcctttggg ctaccaaagg
    26641 cggcttatct taatcccgcc tggtacggcg ctattacgcc gcaggatgca tggacctggg
    26701 catttctcga tctcatcggc caggtgaaat tcctcacgct ttttgcgctg ctgtttggtg
    26761 ctggcctgca aatgttgctg ccccgtggca gacgctggat ccagtcgcgg ttaacgctgt
    26821 tagtcttgct gggctttatt cacggtttat tgttctggga cggcgatatt ctgctggctt
    26881 acgggctggt gggcttaatc tgctggcggc tggtgcgcga tgcgccatcg gtaaaaagcc
    26941 tttttaatac cggcgtcatg ctttatctgg tggggcttgg cgttttgctg ttattggggt
    27001 tgatttccga tagccagact agccgcgcct ggacgccgga tgcatcggct attttatatg
    27061 aaaaatactg gaagcttcac ggcggcgttg aagcgatcag taatcgtgcc gatggtgttg
    27121 ggaacagttt attggcactg ggcgcacagt atggctggca actggctggg atgatgctca
    27181 ttggtgccgc attgatgcgc agtggctggc tgaaagggca gttcagctta cgtcactatc
    27241 gtcgtactgg ttttgtgcta gtggcgattg gggtgatcat taaccttcct gccatcgccc
    27301 tgcaatggca gctggactgg gcgtatcgct ggtgtgcctt cttacttcaa atgccgcggg
    27361 aactgagtgc gccgtttcag gcgattggct atgcgtcgct gttttatggc ttctggccgc
    27421 aattgagccg ctttaagctg gtgctggcga tcgcctgcgt cggacggatg gcgctgacca
    27481 actatctatt gcaaacgctg atttgtacca cgctttttta ccacctcggt ctgtttatgc
    27541 agtttgaccg tctggaattg ctggcgtttg ttattccggt atggctggcg aatattctct
    27601 tctctgttat ctggctgcgt tacttccgcc aggggccggt ggaatggctc tggcgtcagt
    27661 taactttgcg tgctgccgga ccggcaatat ctaaaacatc aagataacga tctggatcac
    27721 attcgttaac aaaacggctg taaccgtttc cattgctgtg actcgattca cgaagtcctg
    27781 tattcagtgc tgacaaaata gccggcagca agcagtcatt tactgcaatc tcataacagg
    27841 tagtgaatat gatcaccatt cgtgatgtag cgcgtcaggc tggcgtctct gtggcaacgg
    27901 tttcccgggt gctcaataac agcacgctgg tcagtgccga cacgcgtgaa gcagtaatga
    27961 aagccgtgag tgagctggat tatcggccaa acgccaatgc ccaggcgctg gcaactcagg
    28021 ttagcgacac cattggcgtg gtggtgatgg acgtttctga tgcgtttttc ggcgcgctgg
    28081 taaaagcggt ggatctggtc gctcagcagc atcagaaata cgtgctaatc ggcaatagct
    28141 atcatgaagc ggaaaaagag cgtcacgcca ttgaggtgtt aattcgccag cgttgtaatg
    28201 cgttgattgt tcactcaaaa gcattgagtg acgatgaact ggcgcaattt atggataaca
    28261 ttcccggtat ggtgttaatc aaccgcgttg tgccggggta cacccatcgt tgcgtttgcc
    28321 tggataatct cagcggtgcc cgaatggcga cgcgcatgtt gctgaataac ggtcatcaac
    28381 gtattggtta tctttcttcc agccacggca ttgaagatga cgccatgcgt aaagcaggct
    28441 ggatgagtgc gttgaaagag caggatatta ttccgccgga aagctggatt ggcactggta
    28501 cgccggacat gccgggcggt gaggcggcga tggttgaact gctggggcgc aatctacaac
    28561 ttaccgctgt atttgcttat aacgacaata tggctgctgg cgcactgaca gcattaaaag
    28621 ataatggcat tgcgattccg ttacatctct caatcatcgg tttcgatgat attcccatcg
    28681 cccgttacac cgacccgcaa ttaacgaccg tgcgttatcc cattgcttca atggcgaaat
    28741 tagccaccga actggccttg cagggggcag caggcaatat tgatcctcgt gccagccact
    28801 gttttatgcc gacgttagtg cgtcgccatt ctgtcgcaac acgccagaat gcggcggcga
    28861 tcactaactc aacaaatcag gcgatgtaac cgctttcaat ctgtgagtga tttcacagta
    28921 tcttaacaat gtgatagcta tgattgcacc gttttaacgt tgtaacccgt atgtaacagt
    28981 gaataatcac tttcaccgag gtaatgcgtc ataacaacaa ttaaagccgt tttctggagc
    29041 gttaccgggc atggaagaac gaattttaaa aagtgagctt cggcgttcag taacacttca
    29101 ttaactctac tgccccgccg agcatttatc tcaagcacta ccctgcataa gaaaaaccgg
    29161 agataccatg aataagaagg tgttaaccct gtctgctgtg atggccagca tgttattcgg
    29221 tgccactgca cacgctgctg atactcgcat tggtgtaaca atctataagt acgacgataa
    29281 ctttatgtct gtagtgcgca aggctattga gcaagatgcg aaagccgcgc cagatgttca
    29341 gctgctgatg aatgattcac agaatgacca gtccaagcag aacgatcaga tcgacgtatt
    29401 gctggcgaaa ggggtgaagg cactggcaat caacctggtt gacccggcag ctgcgggtac
    29461 ggtgattgag aaagcgcgtg ggcaaaatgt gccggtggta ttctttaaca aagaaccgtc
    29521 tcgtaaggcg ctggatagct acgacaaagc ctactacgtt ggcactgact ccaaagagtc
    29581 cggcattatt caaggcgatt tgattgctaa acactgggcg gcgaatcagg gttgggatct
    29641 gaataaagac ggtcagattc aattcgtact gctgaaaggt gaaccgggcc atccggatgc
    29701 agaagcacgt accacttacg tgattaaaga gctgaacgac aaaggcatta aaactgaaca
    29761 gttacagtta gataccgcta tgtgggatac cgctcaggcg aaagataaga tggacgcctg
    29821 gctgtctggc ccgaacgcca acaaaatcga agtggttatc gccaacaacg atgcgatggc
    29881 aatgggcgcg gtagaagcac tgaaagcaca caacaagtcc agcattccgg tgtttggcgt
    29941 cgatgctctg ccagaagcgc tggcgctggt gaaatccggt gcactggcgg gcaccgtact
    30001 gaacgatgct aacaaccagg cgaaagcgac ctttgatctg gcgaaaaacc tggccgatgg
    30061 taaaggtgcg gctgatggca ccaactggaa aatcgacaac aaagtggtcc gcgtacctta
    30121 tgttggcgta gataaagaca acctggctga attcagcaag aaataagact gattgggcgc
    30181 agtctattac tgcgccctta ttgtatggcc aacaaggtaa aattatatgg tcagctcaac
    30241 gactccgtct tccggggaat acttgttgga aatgagcggt atcaacaagt cttttcctgg
    30301 tgttaaggca cttgataacg ttaatttaaa agtccggcca cattctatcc atgcattaat
    30361 gggggaaaac ggcgcaggaa aatcaacatt attaaaatgc ctgtttggta tttataaaaa
    30421 agactccggc accattttat tccagggtaa agagatcgat ttccattcgg ccaaagaagc
    30481 actggaaaat ggtatttcga tggtacacca ggagttaaac ctggtattac aacgttcggt
    30541 gatggacaac atgtggcttg ggcgataccc caccaaaggc atgtttgtcg atcaggacaa
    30601 aatgtaccgc gaaaccaaag cgatttttga tgaactggat attgatatcg atccgcgtgc
    30661 gcgcgtcggc acattatctg tttcgcaaat gcagatgatc gaaatcgcca aagcgttttc
    30721 ctataacgcg aaaattgtga ttatggatga accgacttct tcgttaaccg aaaaagaggt
    30781 caatcatctg ttcactatta ttcgtaaatt aaaagagcgc ggctgcggta ttgtttatat
    30841 ctcgcataaa atggaggaaa tcttccagtt atgtgatgaa gttaccgtat tgcgcgacgg
    30901 tcagtggatc gccaccgaac cgctggcagg gctgacgatg gacaagatta tcgccatgat
    30961 ggttgggcgt tctcttaacc agcgttttcc tgacaaagaa aacaagccgg gcgaagtcat
    31021 cctcgaggta cgtaacctga cgtcactgcg ccagccgtcg attcgcgatg tctcgtttga
    31081 tctgcataaa ggggagatcc tcggtattgc cggtctggtg ggggcgaaac gtaccgatat
    31141 tgttgagacg ttatttggta ttcgcgagaa atcggctggc accattacgt tgcacggcaa
    31201 aaagatcaat aaccataatg ccaacgaagc cataaaccac ggatttgcac tggtaactga
    31261 ggagcgccgc tcaacgggaa tttatgccta tctggatatt ggttttaact cgttaatttc
    31321 caatattcgc aactacaaaa ataaagttgg tttactggat aactcgcgga tgaaacgcga
    31381 tacccagtgg gtgattgatt cgatgcgggt aaaaacaccg gggcatcgga cgcaaattgg
    31441 ttcgctctca ggtggtaatc agcaaaaggt gattattggg cgctggctgt taacgcaacc
    31501 agaaatatta atgctcgatg aaccgacgcg cggtattgat gttggggcga agtttgagat
    31561 ttatcagtta attgccgaac tggcgaagaa aggcaagggg attattatta tctcctctga
    31621 aatgccagag ttgttaggga taacagaccg tattctggtt atgagcaatg gcctcgtttc
    31681 cggaattgtc gatacaaaaa caacaacgca aaacgaaatt ctacgtcttg cgtctttgca
    31741 cctttaagat taggggcttc ccatgagtgc gttaaataag aaaagttttc ttacttacct
    31801 gaaagagggc ggtatttacg tcgttctttt agttttgctt gcgattatta ttttccaggc
    31861 cccaacattt ttaagtctgt tgaacttaag taatattctc acccagtcat cggtgcgtat
    31921 tattatcgcg ctcggtgtgg cagggttaat tgtcacccag gggaccgacc tttctgcggg
    31981 gcgtcaagta gggctggcgg cagtggtggc agcgacatta ttgcagtcca tggataacgc
    32041 caacaaagtg ttcccggaaa tggcgacgat gccgattgcg ctggttattc tgattgtctg
    32101 tgccattggt gcggtgatcg gtttgatcaa cggtctgatt atcgcttatc tcaacgtgac
    32161 gccgttcatt accacgctcg gcacgatgat catcgtctat ggcatcaact cgctttatta
    32221 cgactttgtc ggggcgtcgc caatttctgg ttttgacagt ggcttctcta cctttgctca
    32281 gggctttgtc gcgctgggga gtttccgtct ctcttacatc accttctacg cgttgattgc
    32341 ggtggcgttc gtctgggtgt tgtggaacaa aacccgcttc ggtaagaaca tttttgccat
    32401 tggcggtaac ccggaagcgg caaaagtatc tggtgtcaac gtcggcctga acctgctgat
    32461 gatctacgcg ttgtctggcg tgttctatgc ctttggcggg atgttagaag ccggacgtat
    32521 cggctctgcc accaacaacc tcggctttat gtatgaactg gatgctatcg cggcgtgcgt
    32581 ggtaggcggt gtatcgttca gcggcggtgt ggggacggtg attggcgtgg tgaccggggt
    32641 aattattttt accgtcatca actatggcct gacgtacatc ggcgtaaacc catactggca
    32701 gtacatcatc aaaggggcga ttattatctt cgccgtagcg ctggattcac tgaagtacgc
    32761 gcgtaagaag tgaaatgatt gtggtccgca ttcggtcata aacgcacata acatcatgat
    32821 ctgtgtggtt ttgtaggccg gataaggcgt tcacgccgca tccggcgaca ggagccaaat
    32881 gccggatgca acgctggcgc gttttatccg gcctgcaatg agctcaacat actgatttgt
    32941 atggttttgt aggccggata aggcgttcaa gccgcatcca gcgacaggaa ccaaatgccg
    33001 gatgcgtctc tggttttaca acgttaccgg gtgttctttc tcgcctttct taaacttcac
    33061 ttccccgagc tcaatacaac ccaccgggca gacgtgacca caaagcagac aacccacaca
    33121 tttctcggta ttacaatgcg gtgtgcgggt tttctcgctc cattccatcg cctggtgacc
    33181 gccgtcgtaa caggaaatat aacagcgtcc acagccaaca catttatcaa gattgatacg
    33241 gggatagaca atataactgc ggtctaaatc ttccgccggg acaatattgt tattcgccag
    33301 acctaccatc tcctgcaacg aatcaaatcc ctgatcggcg agataatgcg ataacccgct
    33361 cgccatatct tctacgatgc gatagccgta ctgcataatg ccggtggtga cctgtaacgt
    33421 tgctgcgccg agcaataaaa actccgccgc atcctcccag gtttcaatgc cgccgatacc
    33481 gctgattggg aaatcgcgca gttctggatg ggtgcgcatt tgctgaatga agcgcagggc
    33541 gatcggtttt accgctttac cggaatatcc ggaaatactc gattttccgt taacgatcgg
    33601 cataccgatt ttctgattaa gatcgatatt ggtgatggat ttaacggtgt taatcgccgc
    33661 aatgccatct gcgccgccgc gcttcgccgc cagcgccact tcgcacatat caccgatatt
    33721 cggcgtcatc ttcgccagca ttggcagcgt ggaaccccgt ttcactgccc gacaatattt
    33781 ttctaccagc tccgggcttt gcccgacatc gctacccatc gcatgagaag tcatttgcgg
    33841 acaggagaag ttacactcga tcatatccgc gccagcttct tgcaccaggc gcgccagctc
    33901 ctcccattgc tgctcatttt cccccatgat cgaagcgatc aatactttgt ccgggtaatc
    33961 ttccttcagc cgacgcaggg cggccagatt ctcttccaac ggatgttcag caatctgttc
    34021 catatttttg aagccgataa aaccggtatc ttctttcacc agatgatcaa aacgcggcga
    34081 gacttcgttg gcgataaaaa agccgatcgt tttaaacacc acgccgcccc aacctgtgtc
    34141 gtaggctttg gcacacatct catagcagtt gcctaccggc gaagaagaga ggcagaacgg
    34201 gttgggaaac ttcacgccgc aaaaagtaat cgaaagatct ttcgttaaca tgagcaagct
    34261 ccctctaaat agtgatgaat cgcctcggcg gcttctttcc cggttttcac ggcatagacc
    34321 acggttttgt caccctcaac aatatcgcca gcagcaaaga cttgcggatc gcgggtctga
    34381 taattttgtg ttttgatggt gttacgctgc ggctctaact tcgcaaagtc atccagtctg
    34441 gcatgctgac cgacggcgag aataatttta tctgccgcca tcgtcagttc gcccgataac
    34501 cgtacatgct taaacgtgac tttattgcct tccacggcta ctggcgtgaa tccatcgatg
    34561 atcgaaacgc ccagctcccg ggcgctggca aactcttttt cgcttgccgg aaactcatct
    34621 aactcttcac gcgctacgca agttaccgcc tgacagccga gaactttcag cgtgctggct
    34681 acgtccatcg cgacatcacc gccgccgata attaatgcgc tttgtggaat gctgatatcg
    34741 ccttgcgcct gtcgtgcacg tagcaggaag tcgacggcaa tctcaacgtc gctatgctca
    34801 aacagcggta gcccggaacc gcttgataac cccacggtga ccagtaccgc gcggttttcc
    34861 gctttcagtt gctccagggt gagtgtttta ccgatttcgt tattgcactt aatggtcacg
    34921 cccatttttt caatacgggc gatttctgca tccagcactg attgcggtaa ccgaaattgc
    34981 ggaataccgt tacgcaacca gccaccgggg tgcgcttctt tctcataaat cgtgacgtca
    35041 taaccctggt ttgttagcgt cacactggcc tgcaatccgg caggacctgc gccaataatc
    35101 gcgactttgc cgagcgtttt agtaccgggc tgataaattt ccatcccggt ttgttgttca
    35161 aaatcagtaa caaaacgctg taagcggccg atatcaatgg gagcatcaac accggcacgg
    35221 gtacaaccgc tttggcataa tttctccgtc gggcacactc tggcacaaac ggcaccgagg
    35281 gcattatttt cgcgaattgt ctcggcagcg cctttaaaat tacgaaagta gattgagcga
    35341 ataaatttcc ccggatcggt ctgtgccggg caagcctgac tacagggagc gtcgtgacat
    35401 aataaacagc gagaggcttc tttaatcgcc agtaacggcg taaaagccgg agtgagttca
    35461 tccagataat tttgttgcgg catgtgatct tccttaatgc tatgagtaat taaggcgaac
    35521 gctaaataaa tattgcgtgc tgatattaag gctatgcgcg tggggaaatt taaactgtga
    35581 aaggattcac aaatagctgc ggttcagaat gttatatccg cagcttattg agtgaagtaa
    35641 taattattct caataattaa cagaaaagag aaaatattaa cgatagaaaa taagataaac
    35701 aatcatgccg atagataaaa ttccgaagcc gatatatgcc accatataag cgaaaaagag
    35761 tccgggattt tttgctcgcc agacatacac cggttccggt gcatttttaa cccgtttatc
    35821 aatcgcgcct gcgcgccagc ctttccatgc ttcgcggaaa caaaatatcg ggataaggaa
    35881 aaaaacggca acgacaaaaa actgctgtac atccataact actttccttg tttcttttgt
    35941 aattcaagta actgttcggg tgtgacggcg ggatacccct gcgcatcttc cggcacctgg
    36001 tgcatagccg gaatagggac cagcggccct aaaaaacgcg gttcacgttt aaaaatataa
    36061 aggtcagcca gcgcaccgaa acgggcggca aattcacgaa tacgtactga cagcatatct
    36121 ttcggtgacg gtacggcata acactgagcc tgaatcccca tatgcagcgc aataaataat
    36181 gcccgctcac agtggaaacg ttgggtgata ataatgaaat cattagtatc gaaaactttg
    36241 cgtgtacgca cgatggaatc cagcgtacga aagcctgcgt aatcgagaac aatatctgat
    36301 gggtcgacac cagcagcgat taaatctttg cgcatggtca tcggctcatt ataactttgc
    36361 aatgcgttat cgccgctcag taatagataa tttaccttac cgctgttata ggcattaatc
    36421 gctccttgaa tgcggtagcg ataatactga ttaattacgc cagtacgata atattttgct
    36481 gttccgagca ccacaccgac ctggcggtag gggagatcct gcaattcgtc gtagatataa
    36541 ggcgcggttt tccagctcat ccagcgatcg aggccgagca cagtcaacag cagcaagccg
    36601 atcaggacta acaggctgag gaacacgcgc tttaacatgc aggagactcg atctaaggaa
    36661 aatttccatc aggctacttt acccgctggt tttgcgcaag aaacgacaaa ctgtttgccg
    36721 ggattttcga caccaggaga gggctggcaa gccggacaag gcgttcacgc cgcatccggc
    36781 accaggctta agcgagaagc acgcggtcga tattgtgaca gccgagagct ttcagtgttg
    36841 cggaggtggc atcccactga atcaacggcg catcggcttt ctctgccaga actgcgcgct
    36901 ggatatccgg gtaatcataa cccttgagat tcaatagaat taacgctccc tgcaacggtg
    36961 gcagagtcgg gttgaatgcg gcgttttcag cgtagctgcc gctgaaaata cgaccgtctt
    37021 tacattccag cgcgacaccg cttggcgact tactgtaagg catgtgcgaa cggtttgccg
    37081 ccgcaatcgc cgcctgagaa agcgcatcac cagtcagcgc atagccgtga tcctgctcgt
    37141 ccatcagcag cgttttaatc tccagatctt tcggcccaaa ggcatccgcc agatagtcac
    37201 gcagcgcgtg tgcctcgcgg cccggcagat gaatacgcag atccagaccg ctgttcagtt
    37261 cattcataaa ctgacggcag tgaccacaag gcgtgtagtt aacggtgatg gctgcaagcg
    37321 ctttttcacc actcaaccag gcgtggctga tcgcgctttg ttcggcatga acggtttgct
    37381 gcattgtcgc accaataaat tccatattgg caccaaaata ccaggttccg ctcacaccgc
    37441 gcgcaattgc gccaacatta aaattcgaca atggcgtacg cgcacaggcc gccgccagcg
    37501 gaagtagtgc gaatgccagc gcgtcttcgt ccagccccgt tgcgctcttc agcgatgaga
    37561 cttgctcccc ggtcaacaaa gcggggaagt acttgtctgc cagaataggt tccagtgcag
    37621 attgcaagtt atccgcaagt tgggcaaaag cggtttgaaa acgtggatgc atggcgttgc
    37681 ctcataatca tgtaatggga taacagttta cgaacccgtt atggctttat atgtgatctg
    37741 aatctcatta attatgcaac gcgtgaaact tacatcaaaa atgcgcgacg catcgcaaat
    37801 tttagcccat tactgccaga ataatcggga aaaggatcgg tgcgatcagc gaagtaatta
    37861 tcccgcataa caccagcgct agcgaactaa atgcaccttc ctgataatcc agctcggcgc
    37921 agcgcgccgt accgagggcg tgcgaggcag tccccattgc cagaccgcgc gcagctttgg
    37981 tacgaatacg catcgcatta agcaatgtat ggccaaatac cgcgccgagg atgccgacga
    38041 aaatcacgca aactgcgctg attgccggaa taccgccaat actgccgcca accgccattg
    38101 caataggcgt ggtgactgat ttcggcagga ttgacgcggc gatttccggt gaagcgccca
    38161 tcaacaatgc cacggaagtc cccgtcacca tcgcaaccac gctgccgata aaacagatgg
    38221 tgatgatcga tttccagcgc gcgcggatct ggtgtagctg ctcatataaa ggataggcca
    38281 gcgcgacgac cgccggttgc agcagatcgt ttagcacttc gctaccttta aagtaactgt
    38341 cgtaagagat gccggtcagc attaaaaaag gaatgatcac caccattgct accagcaacg
    38401 ggttaagcag gggaaactta taccgtgccg ccagtttgcg ggcggcaaaa aagacaatca
    38461 acgtcagcgg taatgaccac cagatattcg ccatcattct tctgatcctt tctgacctac
    38521 gactttacgt tcaccgtgta ccagttgcga actccagctc accaccagaa aaaccaccag
    38581 cgtactgatt gcacaggaaa ccactaccgg gccaaactgt gcgcggagca aatcaaaata
    38641 ttgcatgacg cctacgccaa tcggcacaaa caatagcgcc atatagcgaa tcagtacgta
    38701 gcaccccgga ttgacccatt tcgccggaag aatttgcaag gccagcagga caaacaggat
    38761 cagcatcccg atgatgctgc ccggaatggt taccggtagc agggaagcaa tgaaaatgcc
    38821 tgcatacagg caggcataaa tcaggacgaa agcgcgtaaa tattgccaga taatgttcag
    38881 tgtcttactc ataatcaaca ccccagatga gatggattca tcatacaatt aattcgtaaa
    38941 acgtgctacc gatcacatta agaattctca gcatagttga attttaggga ggtaacctcg
    39001 ggcgagcgcc ccgttcagca cactaattct gctgtaaaaa gtgcagaaac tgacttaaag
    39061 cataagaagg cgtttcgtta ttgcgccaga acacaccgac gctacctttc tgttctatct
    39121 ttggcaggtt gagtatcacc agttgtccct ggctggcata acgttgcgcc aggcgtaatg
    39181 acaaaataga gatcatatcg ctgctttgta acaggttggt actgacgttc atcgacgccg
    39241 actcaatcgt gttttctggc agcattacgc cgtggtctac cagcgcatta tcaatactaa
    39301 tgcgaatggg ggtacccgtc ggccagacga tccagcgcca gttggcaaga tcattccagc
    39361 tgagggtggc gtatttcgcc agtgggtgct cagggcgggc gacaaagcat actggctcgg
    39421 tgtacagcac ctgataattc agtggcagct gcaatgcgcg accgccaatt cgcccaacga
    39481 ccacatcgac agaaccggcc aatagatctt gcagcaatgg ggtcatcacc ttctcttcga
    39541 tattcaggtg tagagttggc atcttcgtaa gcaaatgcaa aatcgcctgg gatacgcagt
    39601 cagtggcgac gggagaacag ccaattttca gactgccgac cagtccacct tgtttaaaac
    39661 gggccagttc atgttgcgat cgctccatgt cattgattaa ccgttgggca tgttgcaata
    39721 acaatttgcc gccttcagtc ggacgtaatc ctttgctgtg ccgctcaaaa agtgtgattc
    39781 caatctcatc ttcgagttgt gacaaccatt tcgaaagcgc aggttgggtg atattcatca
    39841 ttcgtgcgac atgggttaga ttgccctgtt cacccagcgc caccagcatt tcaaggtgat
    39901 gtagttttaa tttttgcgcc cagttcgcca tttgccataa cctcaaggtt atctctctgc
    39961 ccgaaatgcc attgtacatc tcatctcttt aatcacaata tcgcaacata tacaaaacaa
    40021 aagaaacatc taccttacct gcactgaggc atttactatg actcaacgac gtgatttaca
    40081 agcacttatc gatgctgctc cggtcggcaa gatgcagtgg cgcgttatca tctgctgttt
    40141 tctggtggta atgctcgatg gttttgacac cgcggctatc ggatttatcg cccctgatat
    40201 tcgcactcac tggcaactca ccgcaggcga tctcgccccg ctttttggcg cgggtctgct
    40261 aggattaacc gcaggcgcac tgctttgtgg cccgttgtcc gatcgctttg gtcgcaagcg
    40321 ggtgattgag ctgtgcgtct ttttgtttgg cgcgttaagc ctcgcctcgg ctttctcacc
    40381 ggatctgcaa acgctggtct tcctgcgctt tctcaccggc ctgggtctgg gcggcgcgat
    40441 gccgaacacc atcaccatga cctcggagta tctgcctgcc cgccgccgcg gcgcgctggt
    40501 gacgctgatg ttttgcggct ttacgctggg ttcggcgttt ggcgggattg tcagtgcgca
    40561 gctggtgccg gtgattggct ggcacggcat tctggtgttg gggggcgtgt taccgctgat
    40621 gctgtttgtc gcactgctgg tagttctgcc ggaatcgcca cgctggcagg tgcgccgcca
    40681 gctaccgcag gcggtgattg cgaaaaccgt cagcgccatc acccgcgagc gttatgtcga
    40741 tacccacttt tatctcattg aatcagcctc cgttacaaaa ggcagcattc gtcaactgtt
    40801 tatgggacgt cagttaccca tcacgttgat gctgtgggta gtgttcttta tgagcctgct
    40861 gattatttac ctgctctcaa gctggatgcc gacgctactg aaccatcgcg gcatcgacct
    40921 gcaacacgcg tccagggtca ccgccgcgtt ccagattggt ggtacgttag gcgcgctggc
    40981 gctgggtgtg ctgatggata agttcaatcc tttccgcgtg ctgacgctga gctatgccat
    41041 aggcgctata tgcatcgtga tgattggctt aagccaggat ggattatggt tgatggcgct
    41101 ggcgattttc ggcactggca ttggcattag cggatcgcag gtggggctga atgcgctgac
    41161 cgcaacactt tatccaacac aaagtcgagc cacgggcgtg agctggtcaa atgctatcgg
    41221 ccgctgcggc gcgattgttg gttcgctgtc tggcggcgtg atgatggcga tgaatttctc
    41281 tttcgacacc ctgtttttca ttatcgctgt gcctgcggcg atcagtgcgg tgatgttaac
    41341 cctgctgatt accgtggttc gccagtcgac ctctgtccct gactcactgc ctcgcgcagg
    41401 cgttgtgaac gaataaggag catctcaatg actgataaca atcaaaatag ccgcgagcaa
    41461 ttttatcagc atatttccgg gcaaaacctg accccgctgt gggagtcact gcaccatctg
    41521 gtgccgaaaa cgcccaacgc taactgtgcg ccagcgtact ggaattatca ggagatccgc
    41581 ccgctgctac tggagagcgg cggcctgatt ggtgcgaaag aggccgtgcg ccgcgtgctg
    41641 gtgctggaaa acccggcgct gcgcgggcag tcttccatta ccgcgacttt atatgccggt
    41701 ttgcaactga tcatgccggg cgaagtggca ccgagtcatc gtcataatca gtcggcgctg
    41761 cgttttattg ttgaaggcaa aggggcattt accgccgttg atggcgaacg cacgccaatg
    41821 aatgaaggcg attttatcct gaccccgcag tggcgctggc acgatcacgg taaccctggc
    41881 gacgaaccgg ttatctggct cgatgggctg gatctgccat tagtgaatat tctgggctgc
    41941 ggttttgccg aagattatcc ggaagagcaa caaccggtaa cgcgtaaaga gggagattat
    42001 ctgccgcgtt acgctgccaa tatgttgccg ctgcggcatc agacggggaa ctcctcgccg
    42061 atctttaact atcgttatga ccgcagccgc gaagtgctgc acgatttaac ccgactgggc
    42121 gatgccgacg agtgggatgg ctacaaaatg cgctacgtca acccggtcac cggcggctat
    42181 ccgatgccgt cgatgggcgc tttcctgcaa ctgctgccga aagggtttgc ctcacgcgtt
    42241 gcgcgtacca ctgacagcac catctaccat gtggtggaag gtagcgggca ggtcatcatc
    42301 ggcaatgaaa ccttcagctt tagtgcaaaa gatatcttcg tggtgccgac ctggcatggc
    42361 gtgtcgttcc agaccacgca agattccgtt ttatttagtt tttcggacag accggtacaa
    42421 gaagccctgg ggctgttccg cgaagcgcgt tattaattta ggagagaaaa atgactcaat
    42481 acgtattcga accgcaagcg cccgttaccg taccggttgt tggcagtgat gaacaattcc
    42541 ccgtgcgccg cgtttactgc gtcggacgta actatgccgc tcacgcccgc gaaatgggct
    42601 ttgatccgga tcgtgaaccg ccattcttct tctgcaaacc ggcggatgcc gttgttccgg
    42661 ttgccgctgg tgaaacgctg gcgctgccgt atccggcaca gaccgataac taccattacg
    42721 aaattgaact ggtggtggcg attggtaaaa aaggcagcga tatcccgctg gaacacgccc
    42781 atgaatatgt ctggggttat gccacgggtc ttgatatgac tcggcgtgac cgtcaaatgg
    42841 aaatgcgcca gatgggccgc ccgtgggaaa tcggtaaagc gtttgatctc tcagcgccca
    42901 tcgcgccgct gcacaaagcc cgcgatatcg gcggtattga taatgcgcca atctggttac
    42961 aggttaacgg cgaagatcat cagcgcagcg atattcgcca tctcatctgg tcggtgaatg
    43021 aaaccatctg ctatctgtca ggcttctttg agctgcaacc gggcgatctg atcttcaccg
    43081 gtacgccgga aggggttggc ccggtagtga aaggtgatgt gatcacaggc aacgtcgagg
    43141 gcttaacgcc tatcgcggtg aagattgtct gaggtgcatg atgaagctgt acagtttttt
    43201 taatagttca gcgtcgtacc gggtacgcat tgcgctggcg ctgaaaggca ttaattacca
    43261 gaccgaaggg gtgaatatcc gtattggtca gcaaaatgaa ctggcctatc ggcggatgaa
    43321 tccggtcggg ctggtgccga cgctgctgac tgacgagggc gaatcactcg gacaatcgct
    43381 ggcgattatt gactggctgg agaggcacta cccgcaagtt ccgctggtgc cgcaggaaga
    43441 gccggcccgc aacaaggtgc tggaaattgt ctatgccatc gcttgtgata ttcatccatt
    43501 gaataacttg cgcgtgctgc gctacctgac cgaagaactg aacgtcagtg aagaagaaaa
    43561 aaaacgttgg tatgcgcact ggatccagca agggttaagc gcggtggaac aactgttgcg
    43621 ccagaaccag tcggggcaat tttgtgtggg cgagacgccg acgctggcag attgctgcct
    43681 tgtcccgcag tgggcgaacg cgctacgcat gaactgcgat ttgagcgggt atccacgctg
    43741 caaagcggtg tacgacgcct gcacacagct gccagcgttc attgccgcag cgccagaaaa
    43801 tcaacaagat aaaatttcag cctgaccagg agaactataa tgacgaaagt gacacgcgca
    43861 gtaattgtgg gaggcgggat cggcggtgcg gcaactgcgc tgtcactggc ccgcctgggg
    43921 atcaaagtgg tgctgctgga aaaggcgcat gagatcggtg agattggcgc gggcatccag
    43981 ttggggccaa atgcgttttc ggcgctggac agcctcggcg tcggcgaagt tgcccgccag
    44041 cgcgcggtgt ttaccgatca cattaccatg atggatgcgg tgaacgccga agaagtggtg
    44101 tgcattgaaa ccgggcaggc ctttcgtgac catttcggcg gcccttacgc ggtgatccac
    44161 cgggttgata ttcacgcctc ggtgtgggaa gcggtactga cgcatccgga cgtggaatat
    44221 cacacctcca ccaacgtcgt ggatattcgc gagacggaag acgatgtcac cgtgtttgac
    44281 gatcgcggca atagctggac cggcgatatt ctgattggct gtgacggcgt gaagtcagtg
    44341 gtgcgccaga gcttattggg cgatgcgccg cgtgtcaccg gacatgttgt ctaccgtgcg
    44401 gttatcgatc gtgccgatat gccggaagat ttgcgcatta acgcaccggt actgtgggcg
    44461 gggccgcatt gtcacttggt gcattaccct ttgcgcggcg gcaagcagta taacctggtg
    44521 gtaacattcc acagccgcca gcaggaagag tggggcgtga aggatggtag taaagaggaa
    44581 gtgctctctt actttgaagg gattcacccc cgtccgcgcc agatgttgga taaaccgact
    44641 tcatggcgac gctggtcaac cgctgaccgt gaaccggtgg cgaagtgggg aacgaagcgc
    44701 ataacgcttg tgggcgattc ggcccatccg gtggcgcaat atatggcgca gggagcctgt
    44761 atggcgctgg aagatgcagt aacgctgggt aaggcgctgg cgcagtgtga gggtgatgcg
    44821 gcgcaagcct ttgcgctgta tgagtcggta cgaattccgc gcaccgcgcg catcgtctgg
    44881 tcgacccgtg aaatggggcg ggtttaccac gccgcagggg tagaacgcca ggtacgtaat
    44941 ctgctgtgga aagggaaatc gcaggcagaa ttttatcgcg gcatggagtg gttgtacggc
    45001 tggaaagaag ataactgttt gcaaccacgc taagcacttc ttcacccaat caggcgcacg
    45061 ggcggtcccc tcgccccttc gggagagggt tagggtgagg gtaaaaatct tgcccgaatt
    45121 aatcagtaag tcagtttgtc tgcaaacaca ggaggcgcta acatagcgcc tcattttttg
    45181 cgggtgatga tatgcgtgtg ttactggctc cgatggaggg agtgcttgac tctctggtgc
    45241 gtgaattgct gaccgaagtt aacgactacg atctgtgcat caccgagttc gtccgcgtgg
    45301 tggatcaact gctgccggtg aaagtctttc atcgcatttg ccctgagcta caaaacgcca
    45361 gccggacacc atccggtacg ctggtgcgcg tgcagttgtt gggtcagttc ccacaatggc
    45421 tggcagagaa cgccgcccgt gcggtggaat taggttcctg gggcgtggat ctcaattgcg
    45481 gctgcccgtc gaaaacggtt aacggtagcg gtggtggggc gacgttactc aaagatcctg
    45541 aactcatcta ccagggtgca aaagcgatgc gtgaagctgt accggcgcat ttgcccgtca
    45601 gcgtgaaagt gcgtctgggc tgggacagcg gtgagaagaa atttgaaatc gccgatgcgg
    45661 ttcaacaggc tggtgctacg gagctggtgg tgcatggacg gacaaaagag cagggttacc
    45721 gcgcggagca tattgactgg caggcgattg gcgagattcg ccagcggctg aatattccgg
    45781 tgattgccaa cggcgaaatc tgggactggc agagcgcgca agaatgcatg gcgatcagcg
    45841 gctgcgactc agtgatgatt ggtcgcgggg cgctcaatat tcccaacctg agccgggtag
    45901 taaaatataa cgaaccgcga atgccgtggc cggaggtggt tgctttgctg caaaaatata
    45961 cccgtctgga aaagcagggc gataccgggt tatatcacgt agcgcggatt aaacagtggt
    46021 tgagttattt gcgtaaagaa tacgatgaag caacagaatt atttcagcat gttcgtgtgt
    46081 tgaataattc ccctgatatt gcaagggcta ttcaggcaat tgatatcggt aagctgcgat
    46141 aatagtggaa tcgcagttta tcagaaacaa tgaatcgctt attcagcttc atcatcttgt
    46201 tgcgctcctt tcatgagcta agccacatat tgccactggc gcaaggagcg cgcaggggga
    46261 ggacaatcgc ctcctccccc tgcacccccg ggctctggcg aacaaaatcg ccgctgcgcg
    46321 gtaccttcag cttatcccct tcggctaccg ggtcgggcgc gaggaaacgt ccttgtaaga
    46381 cgcgccctca gcccacatcc atgtgggc
//
LOCUS       DBFIFI010000016        45482 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.016, whole genome shotgun sequence.
ACCESSION   DBFIFI010000016 DBFIFI010000000
VERSION     DBFIFI010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45482
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.016"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            274..2667
                     /gene="malP"
                     /locus_tag="N7B39_000900"
     CDS             274..2667
                     /gene="malP"
                     /locus_tag="N7B39_000900"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HGU2420925.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLKKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            2677..4761
                     /gene="malQ"
                     /locus_tag="N7B39_000901"
     CDS             2677..4761
                     /gene="malQ"
                     /locus_tag="N7B39_000901"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HGU2420926.1"
                     /translation="MESKRLDNAALAAGISPHYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHITGGKAFNLPT
                     KLPEGYHTLTLTQEDQRGHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVADQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWQAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLETMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(4806..6122)
                     /gene="gntT"
                     /locus_tag="N7B39_000902"
     CDS             complement(4806..6122)
                     /gene="gntT"
                     /locus_tag="N7B39_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HGU2420927.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(6483..7058)
                     /gene="nfuA"
                     /locus_tag="N7B39_000903"
     CDS             complement(6483..7058)
                     /gene="nfuA"
                     /locus_tag="N7B39_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HGU2420928.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            complement(7117..7800)
                     /gene="gntX"
                     /locus_tag="N7B39_000904"
     CDS             complement(7117..7800)
                     /gene="gntX"
                     /locus_tag="N7B39_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HGU2420929.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSLLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAITRTRAT
                     ATQHFLSARLRRRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            7838..8608
                     /gene="bioH"
                     /locus_tag="N7B39_000905"
     CDS             7838..8608
                     /gene="bioH"
                     /locus_tag="N7B39_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312282.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HGU2420930.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLAEMAEAVLRQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRLPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFRHVLVALKQRV"
     gene            complement(8635..9513)
                     /gene="rpnA"
                     /locus_tag="N7B39_000906"
     CDS             complement(8635..9513)
                     /gene="rpnA"
                     /locus_tag="N7B39_000906"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HGU2420931.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYPDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDIT
                     VMPDDEIMQHRRMTLLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASQ"
     gene            complement(9716..9952)
                     /gene="feoC"
                     /locus_tag="N7B39_000907"
     CDS             complement(9716..9952)
                     /gene="feoC"
                     /locus_tag="N7B39_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HGU2420932.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            complement(9952..12273)
                     /gene="feoB"
                     /locus_tag="N7B39_000908"
     CDS             complement(9952..12273)
                     /gene="feoB"
                     /locus_tag="N7B39_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HGU2420933.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            complement(12290..12517)
                     /gene="feoA"
                     /locus_tag="N7B39_000909"
     CDS             complement(12290..12517)
                     /gene="feoA"
                     /locus_tag="N7B39_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HGU2420934.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            complement(12955..15276)
                     /gene="yhgF"
                     /locus_tag="N7B39_000910"
     CDS             complement(12955..15276)
                     /gene="yhgF"
                     /locus_tag="N7B39_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HGU2420935.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLGYLRELEERRQAILKSISEQGKLTDDLANAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIITDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAENEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNDRPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(15373..15849)
                     /gene="greB"
                     /locus_tag="N7B39_000911"
     CDS             complement(15373..15849)
                     /gene="greB"
                     /locus_tag="N7B39_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HGU2420936.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            16077..16796
                     /gene="ompR"
                     /locus_tag="N7B39_000912"
     CDS             16077..16796
                     /gene="ompR"
                     /locus_tag="N7B39_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HGU2420937.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            16793..18145
                     /gene="envZ"
                     /locus_tag="N7B39_000913"
     CDS             16793..18145
                     /gene="envZ"
                     /locus_tag="N7B39_000913"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HGU2420938.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQSTTKEG
                     "
     gene            complement(18221..19843)
                     /gene="pckA"
                     /locus_tag="N7B39_000914"
     CDS             complement(18221..19843)
                     /gene="pckA"
                     /locus_tag="N7B39_000914"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HGU2420939.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTKQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVSEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            20222..21946
                     /gene="yhgE"
                     /locus_tag="N7B39_000915"
     CDS             20222..21946
                     /gene="yhgE"
                     /locus_tag="N7B39_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HGU2420940.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLSGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATLGMSGWLKWQTDGMTPWRAEKALWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYRYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWSELFKLVGITFFKTLFFATDWFIYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFIAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRKGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEHSGRYGRAALESLKSDAEYMKDPKRARDLLMALDGEQHLQQQISEKV
                     LAENVLIAPGSGKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGRAERILFAF
                     DDERYIVYGFDPDKKEWQELTMSLLPRDITKEKLLTAAKDGKLGTKPKAWRDLVVDGE
                     RLDVNLNE"
     gene            complement(22009..22887)
                     /gene="hslO"
                     /locus_tag="N7B39_000916"
     CDS             complement(22009..22887)
                     /gene="hslO"
                     /locus_tag="N7B39_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HGU2420941.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(22912..23313)
                     /gene="hslR"
                     /locus_tag="N7B39_000917"
     CDS             complement(22912..23313)
                     /gene="hslR"
                     /locus_tag="N7B39_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HGU2420942.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(23324..23992)
                     /gene="yrfG"
                     /locus_tag="N7B39_000918"
     CDS             complement(23324..23992)
                     /gene="yrfG"
                     /locus_tag="N7B39_000918"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HGU2420943.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(24057..26192)
                     /gene="igaA"
                     /locus_tag="N7B39_000919"
     CDS             complement(24057..26192)
                     /gene="igaA"
                     /locus_tag="N7B39_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HGU2420944.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHLVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            26512..27072
                     /gene="nudE"
                     /locus_tag="N7B39_000920"
     CDS             26512..27072
                     /gene="nudE"
                     /locus_tag="N7B39_000920"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HGU2420945.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVFEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(27237..29789)
                     /gene="mrcA"
                     /locus_tag="N7B39_000921"
     CDS             complement(27237..29789)
                     /gene="mrcA"
                     /locus_tag="N7B39_000921"
                     /EC_number="2.4.99.28"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HGU2420946.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            29909..30703
                     /gene="hofM"
                     /locus_tag="N7B39_000922"
     CDS             29909..30703
                     /gene="hofM"
                     /locus_tag="N7B39_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HGU2420947.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQRLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGWRLGRRTDEPAN"
     gene            30687..31226
                     /gene="hofN"
                     /locus_tag="N7B39_000923"
     CDS             30687..31226
                     /gene="hofN"
                     /locus_tag="N7B39_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HGU2420948.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTS
                     SAEARVDAVLLQGEQQLARSLQITKPRLLERQQIREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            31210..31650
                     /gene="hofO"
                     /locus_tag="N7B39_000924"
     CDS             31210..31650
                     /gene="hofO"
                     /locus_tag="N7B39_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HGU2420949.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQHWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVFWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            31640..32044
                     /gene="hofP"
                     /locus_tag="N7B39_000925"
     CDS             31640..32044
                     /gene="hofP"
                     /locus_tag="N7B39_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HGU2420950.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTLDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            31956..33194
                     /gene="hofQ"
                     /locus_tag="N7B39_000926"
     CDS             31956..33194
                     /gene="hofQ"
                     /locus_tag="N7B39_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HGU2420951.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRNITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            33595..34116
                     /gene="aroK"
                     /locus_tag="N7B39_000927"
     CDS             33595..34116
                     /gene="aroK"
                     /locus_tag="N7B39_000927"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HGU2420952.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            34173..35261
                     /gene="aroB"
                     /locus_tag="N7B39_000928"
     CDS             34173..35261
                     /gene="aroB"
                     /locus_tag="N7B39_000928"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HGU2420953.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGTFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            35353..36639
                     /gene="damX"
                     /locus_tag="N7B39_000929"
     CDS             35353..36639
                     /gene="damX"
                     /locus_tag="N7B39_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HGU2420954.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            36746..37582
                     /gene="dam"
                     /locus_tag="N7B39_000930"
     CDS             36746..37582
                     /gene="dam"
                     /locus_tag="N7B39_000930"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HGU2420955.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            37600..38277
                     /gene="rpe"
                     /locus_tag="N7B39_000931"
     CDS             37600..38277
                     /gene="rpe"
                     /locus_tag="N7B39_000931"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HGU2420956.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            38270..39028
                     /gene="gph"
                     /locus_tag="N7B39_000932"
     CDS             38270..39028
                     /gene="gph"
                     /locus_tag="N7B39_000932"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HGU2420957.1"
                     /translation="MSKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            39021..40025
                     /gene="trpS"
                     /locus_tag="N7B39_000933"
     CDS             39021..40025
                     /gene="trpS"
                     /locus_tag="N7B39_000933"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HGU2420958.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGDIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASAHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(40156..40887)
                     /gene="frlR"
                     /locus_tag="N7B39_000934"
                     /gene_synonym="yhfR"
     CDS             complement(40156..40887)
                     /gene="frlR"
                     /locus_tag="N7B39_000934"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HGU2420959.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERISAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(40987..41772)
                     /gene="frlD"
                     /locus_tag="N7B39_000935"
                     /pseudo
     CDS             complement(40987..41772)
                     /gene="frlD"
                     /locus_tag="N7B39_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
     gene            complement(41772..42599)
                     /gene="frlC"
                     /locus_tag="N7B39_000936"
     CDS             complement(41772..42599)
                     /gene="frlC"
                     /locus_tag="N7B39_000936"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HGU2420960.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDE"
     gene            complement(42650..43672)
                     /gene="frlB"
                     /locus_tag="N7B39_000937"
     CDS             complement(42650..43672)
                     /gene="frlB"
                     /locus_tag="N7B39_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HGU2420961.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHTIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDARCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPHAEIGKIKNDLKQLPNALGYLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAAISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            complement(43693..45030)
                     /gene="frlA"
                     /locus_tag="N7B39_000938"
     CDS             complement(43693..45030)
                     /gene="frlA"
                     /locus_tag="N7B39_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HGU2420962.1"
                     /translation="MGSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWL
                     TVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDA
                     PSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAK
                     IIPFTIVIGLGIFWFKAENFAAPATTAIGATGSFMALLAGISATSWSYTGMASICYMT
                     GEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPAL
                     GSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSI
                     ILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGL
                     MTPLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
     gene            complement(45326..45481)
                     /gene="yhfL"
                     /locus_tag="N7B39_000939"
     CDS             complement(45326..45481)
                     /gene="yhfL"
                     /locus_tag="N7B39_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020240495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HGU2420963.1"
                     /translation="MKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISISKIIG
                     GCGPTAQ"
BASE COUNT        10737 a        11606 c        12408 g        10731 t
ORIGIN      
        1 actcctttac ttaatttata gagttacctt tcgctttttg aaaatacgca acggccattt
       61 tttgcactta gatacagatt ttctgcgctg tatggcattg atttgctgct aatcctgtgg
      121 tttgcactag ctttaagtgg tcgagatcac atttccttgc tcatccccgc aactcctccc
      181 tgcctaatcc cccgcaggat gaggaaggtc aacatcgagc ctggcaaact agcgataacg
      241 ttgtgttgaa aatctaagaa aagtggaact cctatgtcac aacctatttt taacgataag
      301 caatttcagg aagcgctttc acgtcagtgg cagcgttatg gcttaaattc tgcggctgaa
      361 atgactcctc gccagtggtg gctggcagtg agtgaagcac tggctgaaat gctgcgtgct
      421 cagccattcg ccaagccggt ggcgaatcag cgacatgtta actacatctc aatggagttt
      481 ttgattggtc gtctgacggg caacaacctg ttgaatctcg gctggtatca ggatgtacag
      541 gattcgttga aggcttatga catcaatctg actgacctgc tggaagaaga gatcgaccct
      601 gcgctgggta acggtggtct gggacgtctg gcggcgtgct tcctcgattc aatggcaact
      661 gtcggtcagt ctgcgacggg ttacggtctg aactatcaat atggtttgtt ccgccagtct
      721 tttgtggatg gcaaacaggt tgaagcgccg gatgactggc atcgcagtaa ctacccgtgg
      781 ttccgccaca acgaagcact ggatgtgcag gtagggattg gcggtaaagt gacgaaagac
      841 ggacgctggg agccggagtt taccattacc ggtcaagcgt gggatctccc cgttgtcggc
      901 tatcgtaatg gcgtggcgca gccgctgcgt ctgtggcagg cgacgcacgc gcatccgttt
      961 gatctgacta aatttaacga cggtgatttc ctgcgtgccg aacagcaggg catcaatgcg
     1021 gaaaaactga ccaaagttct ctatccaaac gacaaccata ctgccggtaa aaagctgcgc
     1081 ttgatgcagc agtacttcca gtgtgcctgt tcggtagcgg atattttgcg tcgccatcat
     1141 ctggcggggc gtaaactgca cgaactggcg gattacgaag ttattcagct gaacgatacc
     1201 cacccaacta tcgcgattcc agaactgttg cgcgtgctga tcgatgagca ccagatgagc
     1261 tgggatgacg cctgggccat taccagcaaa actttcgctt acaccaacca taccctgatg
     1321 ccagaagcgc tggaacgctg ggatgtgaaa ctggtgaaag gcttactgcc gcgccacatg
     1381 cagattatta acgaaattaa tactcgcttt aaaacgctgg tggagaaaac ctggccgggc
     1441 gatgaaaaag tgtgggctaa actggcggtg gtgcacgaca aacaagtgca tatggcgaac
     1501 ctgtgtgtgg ttggcggttt cgcggtgaac ggtgttgcgg cgctgcactc ggatctggtg
     1561 gtgaaagatc tgttcccgga atatcaccag ctatggccga acaaattcca taacgtcacc
     1621 aacggtatta ccccacgtcg ctggatcaaa cagtgcaacc cggcactggc ggctctgttg
     1681 gataaatcac tgaaaaaaga gtgggctaac gatctcgatc agctgatcaa tctggaaaaa
     1741 ttcgctgatg atgcgaaatt ccgtcagcaa tatcgcgaga tcaagcaggc gaataaagtc
     1801 cgtctggcgg agtttgtgaa agttcgtacc ggtattgaga tcaatccaca ggcgattttc
     1861 gatattcaga tcaaacgtct gcatgagtac aaacgccagc acctgaatct gctgcatatt
     1921 ctggcgttgt acaaagaaat tcgtgaaaac ccacaggctg atcgcgtacc gcgcgtcttc
     1981 ctcttcggcg cgaaagcggc accgggctac tacctggcga aaaatattat ctttgcgatc
     2041 aacaaagtgg ctgacgtgat caacaacgat ccgctggttg gcgataagtt gaaggtggtg
     2101 ttcctgccgg attattgcgt ttcggcggcg gaaaaactga tcccggcggc ggatatctcc
     2161 gaacaaattt cgactgcagg taaagaagct tccggtaccg gcaatatgaa actggcgctc
     2221 aatggtgcgc ttactgtcgg tacgctggat ggggcgaacg ttgaaatcgc cgagaaagtc
     2281 ggtgaagaaa atatctttat ttttggtcat accgtggaac aagtgaaggc aattctggcc
     2341 aaaggctacg acccggtgaa atggcggaag aaagataagg tgctggacgc agtattgaaa
     2401 gagctggaaa gcggtaaata cagcgacggc gataagcatg ccttcgacca gatgctgcac
     2461 agtatcggca aacagggcgg cgatccgtat ctggtgatgg cggatttcgc agcctatgta
     2521 gaggcacaaa agcaggtgga tgtgctgtac cgcgaccagg aggcctggac tcgcgcggcg
     2581 atcctcaata ccgcccgctg cggtatgttt agctcggatc gctctattcg cgattatcag
     2641 gctcgtatct ggcaggcaaa acgctaagga agctcgatgg aaagcaaacg tctggataat
     2701 gccgcgctgg cggcggggat tagccctcat tacatcaatg cccacggtaa accgcagtcg
     2761 attagcgccg aaaccaaacg gcgtttgctt gacgcgatgc atcaacgtac cgccacgaaa
     2821 gtggcggtaa cgccagtccc gaatgtcatg gtttatacca gcggcaaaaa aatgccgatg
     2881 gtggtggagg gcagcggcga atatagctgg ctgctgacca ccgaagaagg aacgcagtac
     2941 aaaggccata taacgggggg caaagcgttc aatctaccga cgaagctgcc ggaaggttat
     3001 cacacgctga cgcttaccca ggaagaccag cgcgggcatt gcagggtgat tgtcgccccg
     3061 aaacgctgtt acgaaccgca ggcgttgctg aataaacaaa agctgtgggg tgcctgcgtc
     3121 cagctttata cgctgcgatc ggaaaaaaac tggggtattg gggattttgg cgatctcaaa
     3181 gcgatgctag tggatgtggc aaaacgtggc ggatcgttca ttggtctgaa cccgattcat
     3241 gcgctctatc cggcaaatcc ggagagcgcc agcccgtata gcccgtcttc tcgccgctgg
     3301 ctgaatgtga tttatatcga cgttaacgcc gtcgaagatt tccatcttag cgaagaggct
     3361 caggcctggt ggcagttgcc gaccacgcaa cagacgctgc aacaggcgcg ggatgccgac
     3421 tgggtcgatt actccacggt taccgcccta aaaatgacag cattacgaat ggcgtggaaa
     3481 ggtttcgcgc aacgtgatga tgagcagatg gccgcgtttc gccagtttgt tgcagatcaa
     3541 ggcgacagcc tgttctggca ggcagccttt gatgcgctac atgcccagca agtgaaagag
     3601 gacgaaatgc gctggggctg gcctgcatgg ccagagatgt atcagaacgt ggattcacca
     3661 gaagtgcgtc agttctgcga agaacatcgc gatgacgttg atttttatct ctggttgcag
     3721 tggcaggctt acagccagtt tgccgcctgc tgggagataa gccagggcta tgaaatgccg
     3781 attggcttgt atcgtgatct ggcggttggc gtagcggaag gtggggcgga aacctggtgc
     3841 gaccgtgaac tgtattgcct gaaagcgtcg gttggcgcgc cgccggatat cctcggcccg
     3901 ttggggcaga actggggatt gccgccaatg gacccgcata tcatcaccgc gcgtgcttat
     3961 gaaccgttta tcgagctgtt gcgtgccaat atgcaaaact gcggcgcatt acgaattgac
     4021 catgtgatgt cgatgctgcg tttgtggtgg ataccgtatg gcgagacggc agatcagggc
     4081 gcgtatgttc actatccggt ggatgatctg ctctcgattc tggcactcga aagtaaacgt
     4141 catcgctgta tggtgattgg tgaagatctc ggtaccgtac cggtagagat tgtcggtaag
     4201 ctgcgcagca gcggtgtgta ctcctacaaa gtgctctatt tcgaaaacga ccacgagaag
     4261 acgttccgtg caccgaaagc gtatccggag cagtcgatgg cggttgcggc gacacatgac
     4321 ctgcctacgc tacgcggcta ctgggagagc ggggatctaa cgctgggcaa aaccctgggg
     4381 ctgtatccgg atgaagtggt actgcgcggt ctgtatcagg atcgcgaact ggcgaagcaa
     4441 gggctgctgg atgcactgca taaatatggt tgtctgccaa aacgtgccgg gcataaggca
     4501 tcgttgatgt cgatgacgcc gacgcttaat cgtggtttgc agcgctacat tgccgacagt
     4561 aacagtgctc tgttaggact acagccggaa gactggctgg atatggccga accggtgaat
     4621 attcctggca ccagttacca gtataaaaac tggcgacgca agctttccgc aacgcttgag
     4681 acgatgtttg ccgatgatgg cgtgaacaag ttgctgaagg atttggacag acggcgcaga
     4741 gctgcagcga agaagaagta gagtgcggtt gatgccggat gcgatgcatc cggcagtcag
     4801 tgttatcaaa tcaccatatt cagcagcaga cagcctacca gaccgcacac cgagataatg
     4861 gtttccagca tcgaccagga tttgatggtc tcgccgatag tcaggttaaa gtactctttg
     4921 aacagccaga agcccggatc gttcacatga gagaaaatca cactaccgga acccaccgca
     4981 ataaccatca gctccgggct aacacccgtc gttgcaatca gcggtgccgc gataccaccc
     5041 gcagtgattg ccgcaacggt tgcggaaccc agcgcgatac gcagtacggc agcaatcgac
     5101 caggccatca gcagcggaga aatgttggtt tcgtgcatca tggaagcaat gtatttgtcc
     5161 acgccgctgt ctaccagcac ctgcttgaac gcaccgccac caccgatgat caacagcatc
     5221 atcgcaatga ttttgatgga agaaaccagc gtgtcgttaa tctgatccat tgaacgacca
     5281 cggttcagac caaaggtgaa catcgcaatc agcacggcaa tcagcgtagc cattaccggg
     5341 tcaccgagga actccgctac cggcaggaaa gcgtgacctt tcggcaggat catttcggca
     5401 atcgcacgca tcgccatcag tactaccggc accagagagg tccagacgct gacgccaaag
     5461 ctcggcatct cttcttcgct gaaggttttc gcgctgtaga gaccttccgg aattggctta
     5521 tcgatacctt tcaacacgcg agcgtaaacc ggaccggcga gaatcacggt cgggattgcc
     5581 agaatagtac cgtacagcag ggttttaccc atatcggcat tgaaaatggt ggcaatcgca
     5641 gtcggacccg gatgcggtgg caggaagccg tgggtcacag acagtgcagc tgccattggt
     5701 acaccaacat acagcagtgg aatattcgca gaagccgcga tggtaaacac cagcggcagc
     5761 atcagcacaa agcccacttc atagaacagg gcaaaaccaa cggtaaaacc ggtcagtacc
     5821 accgcccact ggatgtgttt tttaccaaat ttggcaatca gcgtggtggc gatacgttgt
     5881 gcgccaccgc agtctgccag cattttgccc agcattgcgc caaaacccat gatcagggca
     5941 aggctaccga gcgtcccgcc gacaccggct ttgatggagc caataacttt atccagcggc
     6001 atcccttgca ttaatccaac cgcaagcgcc acgaggacga gagcgatgaa gccgttcatt
     6061 ttgaagcgga tcatcaggag taataacaag attacaccga tagcaacaat gactaatggc
     6121 atgatttacc tggcctttca tttgttatgg gtaacgtcaa ttttctgacg acaagctcta
     6181 attatcccaa tcgggaacag agatattgcg gcaccacgac tgatacccac taaaactaat
     6241 tattgtagtc agatgtcagg agtatgtttg gtacccacgt gaatgatacg ggtaacatct
     6301 ggcgtttgag aatcaccaga gcggggtaaa tttaaattat gagaggttgg tcatattatc
     6361 gcggggaaac gaagggaggg atttgccaaa gcaatgctgc gccaacgtct ggcacatgtt
     6421 caacgtaggc ccgaaatgac gctttagcgt cgcatcgggc aatctacaaa agaggggata
     6481 acttagtagt aggagtgttc gccgcgctgg tgttcggtga gatcgcgcac acctttcagc
     6541 tccgggaatt cgtttagtag ctgcttctcg atcccttctt tcagcgtcac atcgaccatg
     6601 gaacaaccgt tacagccgcc gccaaattgc agaatggcgt aaccgtcttc ggtgatttcc
     6661 atcagtgaaa cgcggccacc gtgaccagca agctgtgggt tgatctgcga ctgcagcata
     6721 tactccacgc gctccatcag tggtgcatcg tctgccactt tacgcatttt ggcgttaggc
     6781 gctttcagtg ttagctgaga acccaactgg tcggtaacaa aatcgatctc tgcatcttcc
     6841 aggtatggtg cgcttaactc atcaacatac gcggtcagca ggtcaaattt cagggcggtg
     6901 tcggtggctt ccacggcatc cggcggacaa taagaaacac cacattcagc gttaggcgtg
     6961 ccagggttaa tcacaaatac gcggatttgt gtcccttctt cctgatttgc cagcagtttg
     7021 gcaaagtgcg cttgtgcagc atcggaaata cggatcatag taatggccta atagttgact
     7081 attttagttg gttataatac gcccatcatc gaggctctac aaggttcgac aaaggcacca
     7141 gacctggaca gtcgccgcac cattgcgtaa aagcaactgc gcaatctctg cgacggtact
     7201 tccggtggta acgacatcat ccacaatcac catatggcga ccttgcacag gcaattcaag
     7261 gcgaaaggca tttttcaggt tgcgcctgcg tagccgggcg ctaagaaaat gctgggtcgc
     7321 agtggcccgt gtacgtgtga tggcttcgct atcccattgg cagtgcaacc agcgtgataa
     7381 cggctgacac agcaaatcgc tctgattaaa tccccgacgc cagtgacgcc gctgccataa
     7441 cggaacgctg atgatacgat ccggcaattg caacccggtg gtgcgacgag cgtgtaagac
     7501 ttccaatagt aacagacgtg acagggcgct ggcgatttcg ctgcgccggg aaaacttaag
     7561 ctggtggatg agcagactta acggcggcgc atagtcggca accgtgacca gtctttgcca
     7621 gggcggcggt ttttgcaggc agcgaccgca gggaagatgg gagtgtgtgg cgggtaatcc
     7681 acattgtggg cataacgttt tatctgtacg ggcggcgcgt gagcagaccg aacaaatccc
     7741 ccaatgacct aatgccagtg gcattcggca tagccagcat aatcccggta ctgttagcat
     7801 atgttcatcc ttgtaagtca aaagagaaca atagcggatg aataacatct ggtggcagac
     7861 caaaggtcag gggaatgttc atcttgtgct gctgcacgga tggggactga atgctgaagt
     7921 gtggcgttgc attgacgagg aacttagctc gcattttacg ctgcatcttg ttgacctgcc
     7981 cggcttcggg cgtagccggg gatttggtgc gctgtcactt gcggagatgg ccgaagccgt
     8041 gctgcgacag gcacctgata aagccatttg gttaggctgg agtctgggcg ggctggtggc
     8101 aagccagatt gcgttaaccc atcccgagcg tgttcaggcg ctggtcaccg tggcgtcgtc
     8161 accttgtttt agtgctcgtg acgagtggcc ggggataaaa ccggacgtgc tggcgggatt
     8221 tcagcagcaa ctcagtgatg attttcagcg tacggtggaa cggttccttg cgttacaaac
     8281 catgggtact gaaacggcgc gtcaggatgc gcgggcgttg aagaaaaccg ttctggcgtt
     8341 accgatgccg gaggttgacg tgctcaatgg tgggctggaa atcctcaaaa cggttgatct
     8401 ccgcttgccg ctgcaaaacg tacccatgcc gtttttgcga ctgtatggct atctcgatgg
     8461 tctggtgccg cgcaaagtgg tgccgatgct ggataaactc tggccacaca gcgagtcata
     8521 catcttcgcc aaagcggctc atgcgccgtt tatttcgcat ccggtcgagt ttcgtcacgt
     8581 gctggtggcg ttgaagcaga gggtgtaggc acttttgaat ggcgagacag aactttactg
     8641 actcgccgca gccaactctt cttctgacag cccggtaaag cgcatgatgt ctgcaagagg
     8701 gactccggat tcaagcatta ttttggctat atgcagggct ttggattgtt ccccctcctg
     8761 ccgcaatctt tccgcaatag tcattaaact ctccttgtgt ttcggtgatc gttcggcaac
     8821 gccgtcgata aaatcgttaa aacgtaccgc atcgccggtt tgcagtatgt aattaaacag
     8881 ccctttgatt tgtctgtcat tagcgtatcc actacttaat aagcaggcca tttgctctac
     8941 cagccccatc aggtcgcgtt gacgaatatg tttttgaatt aactccagca gcgtcatgcg
     9001 tcggtgctgc atgatttcat catcaggcat gacggtgata tcaatcagcg gaaatgcgga
     9061 ggcataaagc tgccttgcca gtttgggatc ggcgaaacaa tccagccaac acagcgaata
     9121 gggatagggg ctttcaatac cgtggtaaaa caacaatggc accaccatcg gcaacgtttt
     9181 gtatccggca tcaaggtgat tttgcattgc ggcaatagcg taacgcatca tgcgaaatgc
     9241 gatcagtttg tttgaggtgc tttgatgttc aatcagacaa tagatgtatc ctggtccttg
     9301 ttctgttttc accgaccaca gcacatcggg atagctttca cgcagatcgt catcaataaa
     9361 gctgctcgac tccagcttga gggttttcat atcacagagc gcgtgaatgg gtgctggtaa
     9421 atgaaacgca agaaaatcgc gtgccgtctc cggttggcgt aaaaagagtt tgaacagcgc
     9481 atcgtgtggg gtggaactct gctttttgct catggcgttc cttcacctgt tgtttgaatg
     9541 ccgcgactct acgcgccact ccacacctgt gcattagcca gttttcatcc ctgcgagacg
     9601 ctttcagagg aacttacgtc gcgctgcaag aattgcattt ttatggaaag ccgcacgcaa
     9661 aaaagcccgg atgcaaacgc ataccgggct tttcaaaaca ggcgatggag tatggttaac
     9721 gcagcgccca ccactcgcgc agacaggctt ttccttccgg gcagctttta caactgccgg
     9781 agaggcagcc gtcaggttct tcctgaatcc gcacggcttt gcccatactt tccagttgtt
     9841 gcagcatggc gttaatcatt ggctgtggag tgttcaatgt ctggcttatc tgggccgctt
     9901 ccatacggcc ccgtaacgcc agcaaatcac gcacctgaat aagtgaagcc attaatggca
     9961 atcaccggtg gtgctggctg cgcagcaact acttaccgac ttgcgggttg ccagcagttc
    10021 gatatccacc cggctacgcg cgcggcgcag cagaccgata accacgatgt taaacaggat
    10081 aaccgccaga atgcacacca ggctgtaagt tgggtgctga ctgtagctgg cgacttgata
    10141 gaacaatgtt gccagtgagt aagcgatatt cagcccccac aggatggaga agcccatcca
    10201 gccacggctt gattcacggg cgatggctcc catcaccgag atacatggta catacagcag
    10261 gacgaaaatc aggtagctgt atgcggcagc tgcgctaccg aatttctgat ccatcacgcc
    10321 catcgccccg gtacccattt cgccgtcgcc tttgctagct tcaatggggt tcatcagtac
    10381 gctaaggctg aaggtgtctt tcaggctctg ccaggtttca tctaccgcgc tgaacaactc
    10441 ttcaccgagg ttaaattccg ccggattgaa ctcttcgtcc tgaatatttt ctgcggtgta
    10501 gagggtgttg agcgtaccca ccaccacttc tttcgccatt gcacccgtaa acaggccaac
    10561 tgttgcctgc cagttatctt catgcacgcc aatcggcttg aagaccgggg tgatcacccg
    10621 gctgacggaa gccagcgccg agtcgttgat gttatcgacg attttcccgc tcaacgagaa
    10681 gctgttgaaa gcgctcagga aaatgctgac gatgataatc actttaccag cacgcagaac
    10741 gaagcctttc agacgctgcc aggtctggat aatcaggctt ttaacgtgtg gtacatggta
    10801 gaccggcagc tccatgacaa acggcgtcgc ttcaccgcgc atgatggtgt acttgagcat
    10861 caggccagtc agcaccgcca tcacgatacc gagcatatac agcgagaaga ccgccagcgc
    10921 accgttctgc ccgaagaagg cagccgcgaa tactgcgaag atagccagac gcgcgccgca
    10981 ggacataaac ggtgccatca tgatggtcat cagacgttca cgcggtgcat caagcgtacg
    11041 tgcacccatt accgacggta cgttacaacc gaaaccgacg atcagcggca caaaggattt
    11101 ccccggcaag cccagcgcct gcatcaggcg gtccatcaca aacgccgcac gcgccatata
    11161 gccggagtcc tcaaggaagg agaggaacag gtacatcatg ccgatctgcg gcaccagtgg
    11221 cagcacggtg ttaataccgc cacccaggcc ctgggcgagg aagatagtca gccagtccgg
    11281 gaagtggagt gtgtagccaa tccattgaat accatgcaca aatagagcca cggagccgac
    11341 gtcaaacagc ggctgtaacg ccccgccgat gttgatagcc agcaggaaca tcaggtacat
    11401 cacaaagagg aaaatcggca gaccgaggaa acggttgagc acgattttat ctaccgcagt
    11461 ggtgaaacgg ctgggttctg ccgtcagggt gttgcttacc acatcacaga tggcagcaat
    11521 gcactggtaa cgcgcatcgg caatgtgcag cgccggatcg tccatctcat tacgcagacg
    11581 ggcgagggcg gcatcaagat gctgcgacgc ttcaccggcg taggcgcggc tgtagatatc
    11641 gccttccagc atttgcaggc ccagccagcg acgttgtttc agcgggatgt cggaaggcat
    11701 cacttttgcc agtgaatctg cttcgttgag tagcggctgt gcgtaatgca ccagttccac
    11761 attctcgtta gctttatagc gatcaatcgc cagcttgagc gcttcaatac cgcgaccacg
    11821 ggttgaaacc agcgggatca ctgggcagcc aagacgcgcc gacagagcat caatttcaat
    11881 acgaatattt tgcttctcgg caatgtcgag catgttcagt gccacgatgc agggaatgcc
    11941 gagttccagc agttgtagcg tcaggtacag gttacgctca aggttagacg catccaccac
    12001 gttaatcagc aggtcggcgt cgccactcaa aatgtagtga caggcgattt gctcatcgag
    12061 cgaggtctgc gatgagatgg tggtcagaga ataggtgccg ggcaggtcca ccagcgtgac
    12121 ctgatgatcg gtggtggaga attgcccttc tttacgttcg acggtaacgc cagcccagtt
    12181 acctacacgc tgacgtgcgc cagtgagctg gttaaataag gttgtcttgc cagaatttgg
    12241 attaccaatt aagcctatgg ttaatttttt cattgttgtt atcaccgtat taacaggaaa
    12301 ccgcttccac ttctaataag gccagatctt ttttgcgtaa taccaggctc acacgacggg
    12361 tttcgatatg aatggggtcg ccgagtggag cgacgcgcac cacattaaaa gaggagccag
    12421 gtaacatgcc aagagaaagc agtttttggc gatatgccgg gctgatttca cgggaaaagc
    12481 cagtgatttt ccacgcagta tctggagtgt attgcatagg tgcctacttg tttctcatta
    12541 actggataac tacctcatgg ggcgtttgac agcgacatga gaccagatga tttgccaacg
    12601 aaggagagat taaataatca atagtacaat gataatgaga atggtttcta tcagcaatac
    12661 ttaaaatgtg tgcgaatgtt ggtttgaata ataatctgac tcaatgttga tgtggctcaa
    12721 ggttatatcg cagcgaaaat ataattcgaa tagcgaatta ccaggactgg ctaatgttta
    12781 attaaggttt cagttgttaa tgaagtgata aatattaacg aaatatattg tgagtttggc
    12841 tgtggaataa atatgccgga gatattctcc ggcactttta tgtcaggcaa ggcgttcgtt
    12901 gccagatgcg gcgtgaacgc cttatcgcgc aggtttaggg gccagtccgg gcgtttaacg
    12961 ttttttgccc attgctgccg ccagcgcgtc catcatcgcg ctgttaccgg caggctgcgc
    13021 ttcacgaccg cgtggtttgg ctgccgggcg gttgttttgc gggcgatcgt taccgccgcc
    13081 gcgacgagag ttggtttcgc caggctgctc gtccagacgc atagtcaggg cgatacgttt
    13141 acgctgaaga tccacttcca gcactttcac cttcacaatg tcgcccgctt tcaccacggt
    13201 atgcggatct tccacaaact tgttcgacaa tgaagagatg tgaaccaggc cgtcctgatg
    13261 cacgccgata tcgacaaacg cgccaaagtt ggtgacgttg gtgacagcgc cttcgaggat
    13321 catacccggt tgcaggtcgt tcattgtctc gacgccatcg gcaaactgag cggttttaaa
    13381 ttccggacgc ggatcgcgac ccggtttttc cagctctttg atgatgtcag ttactgtcgg
    13441 cacaccgaat ttttcatcag taaagtcaga cgctttcagg ttacgcagtt cgctgctgtt
    13501 acccatcaga tctttcagtg cctgctgtgt tgctgccaga atgcgttcca ccaccggata
    13561 ggcttccggg tgaacggtag aggcgtccag cgggttgtca ccgtggttaa tgcgcaggaa
    13621 gcccgcgcac tgctcgaagg ctttcggccc cagacggctg actttcagca gttgctgacg
    13681 gttctggaac tggccgttct catcgcgcca ggccacgatg ttttgcgcca tcattcgcgt
    13741 caggcccgcc acgcgagtta atagcggaac agaagcggtg ttgagatcga cgccaacggc
    13801 gtttacgcag tcttctacta ctgcatccag tttgcgggcc agttgcgtct ggctgacgtc
    13861 atgctgatac tgacctacgc cgatagattt cggatcgatt ttcaccagct ccgccagcgg
    13921 atcctgcaaa cggcgggcga tagaaaccgc gccacgcagc gaaacgtcga gatccgggaa
    13981 ctcctgcgct gccagctcgg aagccgagta aaccgacgcg ccagcttcgc tgacgatcac
    14041 tttctgtgcg gtcactttcg ggaactgctt ctgcacgtcg agatagaaac gttcggtttc
    14101 gcgggaagct gtaccgttgc cgatcgccac cagttcaacg ttatgctttt cacacagcgc
    14161 agcaacagtc atcgctgctt ttgcggcttg tccggtgtgc gggtaaatgg tatcggtcgc
    14221 taccagtttg ccagtggcat cgaccaccgc cacttttacc ccggtacgca gacccggatc
    14281 gaggcccatc gttgcacgca gtccggcagg ggcagccatc agcagatcgt gcaggttacg
    14341 ggcaaagacg ttgattgctt cattttccgc acgttcgcgc acggtgccca tcagttcggt
    14401 ttccagatgc atcagcacct tgatgcgcca cgtccagctc accacgcctt tgcgccagct
    14461 atccgccggg gcattgttca ggcgcaggcc aaggtgatcc gtgatgattt gctcgcaata
    14521 gctctctttg ggcggctcat cgaactgtgg atcggcattc agcgaaagct gaagtacgcc
    14581 ttcgttacgc ccacggaaca tcgccagcgc gcggtgagaa ggcaccgtgg acaacggttc
    14641 gtgatgatcg aaatagtcgc ggaatttcgc cccttcctct tctttaccgc tcaccaccgt
    14701 agaaaccaaa tgcgcgttct tccacagata atcacgcact ttcgccagca gcgcggcatc
    14761 ttcggcaaac cgttccatca ggatatagcg cgcgccgtcc agcgcggctt tggtatctgc
    14821 cacgccttta tcggcatcaa tatattgcgc agcggcgact tctggcgtgt gtgacggatc
    14881 gctccacagc aggtcagcca acggctcaag ccctgcttca atggcgattt gcccgcgggt
    14941 gcggcgttta ggtttgtagg gcaggtagag gtcttcgagt tcggttttgc ttagggtggc
    15001 gttgatggca ttcgccagat catcggtgag tttgccttgc tcggaaatgg acttgaggat
    15061 cgcctgacgt ctctcttcca gctcgcgcag gtatcccaga cgcgtttcca gattacgcag
    15121 ctgcgtgtca tccagaccgc cggtgatttc cttacgataa cgtgcaataa aaggcacggt
    15181 attcccttcg tcaagcaggc gaacggcagc gtcaacctgt tccgggcgcg cctgaatttc
    15241 acccgcaata atgcggcaga acgaatcatt catcatggtt tgagttcatc ttttcggatc
    15301 aaaaatcagg ggatagttat acggactggc tggcaaaaat gccagccatc ggcaggaggt
    15361 taagactctt ccttacggtt tcacgtactc gatagcatta acataccagc tcgcttcccc
    15421 ggcaggagta ttcaccacag ccagatcgcc gacttctttt ttcagcaatg cgcgggccat
    15481 cggggaatcg atagagatgt aatctttacg gccaaaaatt tcatcgtagc cgacaatacg
    15541 gaaacggtga gtcacgccat cgtcgttttc aatctccacc cacgcgccaa aaaagacttt
    15601 gccttcctgc tgcggggagt aatcgacgat tttgagattt tccatgcatt tagtgagata
    15661 gcgcacgcga cggtcgattt cacgcaggcg ctttttatta tactgatagt cagcattttc
    15721 gctgcggtcg cccagacttg cggcccaggt cacctttttt gtgacctccg ggcgttcttc
    15781 acgccagaga taattaagct cttgtttgag tttttcatac ccttcccggg taaccagggg
    15841 cgttttcatc tcgttgattc cctttgtctg tttgataatg cgcacattgg gtataacgtg
    15901 atcatatcaa cagaatcaat aatgtttcgc cgaataaatt gtatacttaa gctgctgttt
    15961 aatatgcttt gtaacaattt aggctgaaat tcataccaga tttagctggt gacgaacgtg
    16021 agctttttta agaatacacg cttacaaatt gttgcgaacc tttgggagta caaacaatgc
    16081 aagagaacta caagattctg gtggtcgatg acgacatgcg cctgcgtgcg ctgctggaac
    16141 gttatctcac cgaacaaggc ttccaggttc gaagcgtcgc taatgcagaa cagatggatc
    16201 gcctgctgac tcgtgaatct ttccatctta tggtactgga tttaatgtta cctggtgaag
    16261 atggcttgtc gatttgccga cgtcttcgta gtcagagcaa cccgatgccg atcattatgg
    16321 tgacggcgaa aggggaagaa gtggaccgta tcgttggcct ggagattggc gctgacgatt
    16381 acattccaaa accgtttaac ccgcgtgaac tgctggcccg tatccgtgcg gtgctgcgtc
    16441 gtcaggcgaa cgagctacca ggcgcaccgt cacaggaaga ggcggtaatt gctttcggta
    16501 agttcaaact taacctcggt acgcgcgaaa tgttccgcga agacgagccg atgccgctca
    16561 ccagcggtga gtttgcggta ctgaaggcac tggtcagcca tccgcgtgag ccgctctccc
    16621 gcgataagct gatgaacctt gcccgtggtc gtgaatattc cgcaatggaa cgctccatcg
    16681 acgtgcagat ttctcgtctg cgccgcatgg tggaagaaga tccagcgcat ccgcgttaca
    16741 ttcagaccgt ttggggcctg ggctacgtct ttgtaccgga cggctctaaa gcatgaggcg
    16801 attgcgcttc tcgccacgaa gttcatttgc ccgtacgtta ttgctcatcg tcaccttgct
    16861 gttcgccagc ctggtgacga cttatctggt ggtgctgaac ttcgcgattt tgccgagcct
    16921 ccagcagttt aataaagtcc tcgcgtacga agtgcgtatg ttgatgaccg acaaactgca
    16981 actggaggac ggcacgcagt tggttgtgcc tcccgctttc cgtcgggaga tctaccgtga
    17041 gctggggatc tctctctact ccaacgaggc tgccgaagag gcaggtctgc gttgggcgca
    17101 acactatgaa ttcttaagcc atcagatggc acagcaactg ggcggcccga cggaagtgcg
    17161 cgttgaggtc aacaaaagtt cgcctgtcgt ctggctgaaa acctggctgt cgcccaatat
    17221 ctgggtacgc gtgccgctga ccgaaattca tcagggcgat ttctctccgc tgttccgcta
    17281 tacgttggcg attatgctat tggcgatagg cggggcgtgg ctgtttattc gtatccagaa
    17341 tcgaccgttg gtcgatctcg aacacgcagc cttgcaggtt ggtaaaggga ttattccgcc
    17401 gccgctgcgt gagtatggcg cgtccgaggt gcgttccgtt acccgcgcct ttaaccatat
    17461 ggcggctggt gttaagcaac tggcggatga ccgcacgctg ctgatggcgg gggtaagtca
    17521 cgacttgcgt acaccgctga cgcgtattcg cctggcgacc gagatgatga gcgagcagga
    17581 tggctacctg gcagaatcga tcaataaaga tatcgaggag tgcaatgcca tcattgagca
    17641 gtttatcgac tacctgcgca ccggacagga gatgccgatg gaaatggcgg atctcaacgc
    17701 agtactcggt gaggttattg ctgccgaaag tggctatgag cgggaaattg aaaccgcgct
    17761 ttaccccggc agcattgaag tgaaaatgca cccgctgtcg atcaaacgcg cggtggcgaa
    17821 tatggtggtc aacgccgccc gttacggcaa tggctggatc aaagtcagca gcgggacgga
    17881 gccgaatcgc gcctggttcc aggtggaaga tgacggtccg ggaattgcgc cggaacaacg
    17941 taagcacctt ttccagccgt ttgttcgcgg cgacagtgca cgcaccatta gcggcacggg
    18001 attagggctg gcgattgtgc agcgtatcgt ggataaccat aacgggatgc tggagcttgg
    18061 caccagcgag cggggcgggc tttccattcg cgcctggctg cctgtgccgg taacgcgggc
    18121 gcagagcacg acaaaagaag ggtaaataaa cgggaggcga aggtgcctcc cgttttgctt
    18181 tctataagat actggataga tattctccag cttcaaatca ttacagtttc ggaccagccg
    18241 ctaccagcgc ggcacccgca ggggtgtcgg tgtatttatc aaagttgtcg ataaacagtt
    18301 tcgccagggt ttcggctttt tcctgccact gttccgggga agcgtaggtg ttacgcggat
    18361 cgagaatctt cgtgtctacg cccggcagtt cggttgggat cgccaggtta aacatcggca
    18421 gagtgaaggt ttctgcatta tccagcgaac cgttgaggat ggcgtcgata atggcgcggg
    18481 tatctttaat cgagatacgt ttgccagtgc cgttccagcc agtgttaacc agataagcct
    18541 gcgcgcccgc cgcctgcata cgtttcacca gcacttctgc gtactgagtc gggtgcagcg
    18601 acaggaatgc cgcgccgaag caagcggaga aggttggcgt cggttcggtg atgccacgct
    18661 cagtaccggc cagtttggcg gtgaagccag agaggaagtg gtactgggtt tgatcggcag
    18721 tcagacgaga aaccggtggc agcacaccaa aggcgtctgc ggtcaggaag ataactttcg
    18781 ttgcgtgacc cgcttttgaa accggcttaa cgatgttatc gatgtgatag atcggataag
    18841 aaacgcgggt gttctcggtt tttgaaccat catcaaagtc gatagtgcca tcttcacgca
    18901 cggtgacgtt ttccagcagc gcatcgcgac ggatagcgtt gtagatttca ggttccgctt
    18961 ctttcgacag cttgatagtt tttgcgtagc agccgccttc gaagttaaac acgccgtcat
    19021 cgtcccagcc gtgttcgtca tcgccaatca ggcgacgttt cgggtcggtg gaaagggtgg
    19081 ttttaccggt gccggaaagg ccgaagaaca ccgcaacatc gcctttctcg ctaacgttgg
    19141 cggagcagtg catagaagcg atacctttca gcggcagcag gtagttcatc atcgagaaca
    19201 tccctttctt catttcgccg ccataccagg tgccgccaat cagctgcatg cgctcggtca
    19261 ggttaaaagc cacgaagttt tcggaattca gaccctgttc tttccactgc gggttagtgc
    19321 acttcgcgcc gttcataacg ataaagtctg gtttgaaacc tgccaactct tcatcgctcg
    19381 ggcgaataaa catgtttttg acaaaatgcg cctgccaggc cacttcggtg atgaaacgga
    19441 cggaaagacg agtatccggg ttcgcaccac agaaggcgtc gacaacgaac agacgtttgc
    19501 cggaaagctg tttggtcacc aggcctttca gatgttgcca ggtttccgga gagagaggtt
    19561 tgttatcgtt cttacctttg cctttgtctg cccaccagaa agtatcgcga gtggtatcgt
    19621 cacggacgat atacttatct tttggtgaac gaccggtgaa gatcccggta tcgacggcaa
    19681 cggcacccag attagttaac accccgcgct cataacctgt caggctcgga tcgagctctt
    19741 cctgatacag caggtcgtag cttgggttgt aaacgatatc atgtacgtca ctgataccat
    19801 aagcctcgag ttcttgcggg gtcaaaccat tgttaacgcg catttcactg ctccttagcc
    19861 aatatgtatt gcctgaatag taaagtcttt ttgggggtgt taaccgcgac aaggctcata
    19921 gatttacgta tctggagaaa ttcatatttg acaaaaaact ccgtgattcc tgtcacgaaa
    19981 cggttgctat tatcgcagaa atagcatttt aggtgtggaa aatgttctca aaaggttaaa
    20041 tcttggttta tatggcggga atattgtgag tggaatcgca ttcctgaatg aaattgatgc
    20101 aaattcaacc ggtaaagtat cgattcacca cgctaacttt ttttgattat tttctgaaaa
    20161 aagcaaaata ctactgcccg gatgcgataa ttaacccacc ttttgattct ggataaccaa
    20221 catggataac gttgaacttt cacccgcaac acgctggggg atgattgcta ccggattact
    20281 tcagggtctg gtttgctacc tgctgatcgc ctggctttcc ggaaaaaatc acagttggat
    20341 tgtttatggc gtgcctgcga ccgtcgcgtt ttcatccgtt ttactcttct ccgtgatctc
    20401 ttttaaacag aagcgccttt ggggatggct ggcgctggtg tttattgcca cgctggggat
    20461 gagcggctgg ctgaagtggc aaactgatgg catgaccccc tggagagctg aaaaggcgct
    20521 ttgggatttt ggttgctatc tgctgctgat ggcaatgttg ttgctaccgt ggatacaaca
    20581 aagccttcgc atccgcaatg acagtagccg ctatcgctat ttttaccagt cagtatggca
    20641 taacgtactt atattactgg tgatttttct cgccaatggt ctgacgtggc tggtgctttt
    20701 actgtggagt gagttgttta aacttgttgg catcacgttt ttcaaaacgc tcttttttgc
    20761 aaccgactgg tttatttatc tcacgttagg tctggttacc gcgctggcgg tgatcctcgc
    20821 gcgaacacag tcacgtttaa tcgactctat tcaaaagttg ttcacgttaa tcgccacggg
    20881 gttgctgccg ttagtatcat tgctaaccct gatgtttatc atcaccctgc cgtttacagg
    20941 cctgagtgcg atttctcgcc acatctccgc cgccgggttg ctgttaacgc tggccttttt
    21001 gcaattgatc ttaatggcta ttgtccgcga tccgcaaaaa gcgtcacttc cctggacggg
    21061 gccgttgcgt tgcctgatta aaaccgctct gctggttgct ccgctgtatg tgttcatcgc
    21121 cgcctgggcg ttatggctgc gggtcgctca gtacggctgg actgtcgacc gcttgcaggg
    21181 cgcgctggcg gtgctggtgt tactggtatg gtcgctgggg tattttgtca gtatcgtctg
    21241 gcgtaaaggg caaaatcccc ttgttcttca gggcaaagtg aaccttgcgg tttcgttatt
    21301 ggtgttggta atactggtgc ttcttaattc gccggtgctg gacagtatgc gcattagtgt
    21361 gaacagtcat atggcgcgtt atcagagcgg caaaaacacg ccagaccagg taactatcta
    21421 catgctcgaa cacagcggtc gctatggacg tgcggcgctt gagtcgctga aaagcgatgc
    21481 cgagtatatg aaagacccga aacgcgcgcg ggatctactg atggcgttag atggagagca
    21541 acatcttcag caacagatat cggaaaaagt attagccgaa aatgtgttaa ttgcccctgg
    21601 ttctggtaaa ccggatgcga cattctggtc ggccttaatc caggatcgct ataacgtgat
    21661 gacctgtatt gaaaaagacg cctgcgtact ggtcgagcaa gatctgaata gtgatggcag
    21721 ggcagagcgg atcctctttg cctttgatga tgaaagatac attgtctatg gctttgatcc
    21781 tgacaagaaa gaatggcaag agcttacgat gagtttactt ccgcgtgata taacgaaaga
    21841 aaaattactc acagctgcga aggatggaaa actgggaacg aagcctaaag cgtggcgcga
    21901 tcttgtagtg gatggtgaaa ggctggatgt gaatctgaat gagtgacaaa gcgcatcagg
    21961 tgtattagtc gtaaaatccg gcagagctct ctctgccgga catactcatt aatgaacttg
    22021 cggatctgcc ggagacgcgt tgttgcggat ttcagcaata tccatcgcat tgaacagata
    22081 gtggttaccg cagtaatcac aatgcatgtc aatttcgcca tcttccgcca ggatgctatc
    22141 gacttcttca tcaggtagcg ttttcagcgc atcggcgcaa cgttcacgcg agcaggtgca
    22201 tttgaactcc acatcctgcg gatcgtaaac cgtcacctct tcttcgtgat acaaacgcca
    22261 caacacttcg tttgccggta aggtcagcag ttcttcggtt ttgatggttt cggttagcgt
    22321 cgccaggtgg tcaaagtcgt cctgctgggc attttgcgca ggcattacct gcaacaacat
    22381 accgcctgca gccggtttgc cgtctacgtc gccggtgcga ataaacaggc gcgtcggcag
    22441 ctgttcagaa cgcataaagt aatcttccag gcaggccgcc agggtatcac cttccagacc
    22501 caccacgccc tgatagcgtt caccttcgct cggggtaatg gtgatcacca cgtaaccatt
    22561 gccgaccagc gttttcaggt cggcattttc tggaatttcg ccctgcacgc gcgccacacc
    22621 gcgcatctgc tggttgttgt taccgttaat aaccgccaga ttcatcggac cgtcgccttg
    22681 cagctgtacg gtgatatcac catcaaactt cagcgtagcg gttaacaggc tggtcgcaac
    22741 cagcagttct gccagcacgt ttttaacggg ctgcggataa tcgtgattct caaggatctg
    22801 ttgcagggtt tccgaaacgg ttaccagttc gccgcgcacg gcaaagtttt caaacagata
    22861 gcgatgtaat tggtcatgtt gcggcataat catctctctt gcaggtgaca gttattcact
    22921 gtcgccgtgt ttaaatcgta acaggtcgcg gcgctctttt ttgtccgggc gtcggtccgg
    22981 gtgcggcatg gttaaggcat taagtttacg tgccagcgcc attttttcgc gtttctctac
    23041 actttccgca gtctcttcat acagcaaggc tgcctcgctg gcggggcgac gctgttcagt
    23101 aatcgccttt acaatcaccg tgcgttcgtc atttccctgg cgcagagtga gcgtggcatt
    23161 cagctcgacg attttgctcg gcttgctgcg ctgcccgttg taatgcacct taccgccttc
    23221 aatcatttca cgggccagcg cgcgggtttt ataaaaacgg gcagcccata gccatttatc
    23281 cagtcgaacc tcaacagcag gtttttcttt catggcgtct ccttcacatt agcgagggga
    23341 tcaggcggcg gtagtcattc agtgacggat ggcgttgata ctgtttctcg gcaatccctg
    23401 agtcaggatt agtcacgccg aggcagtaac gaataccaaa ttgcgcggca gcatcgagaa
    23461 tcgcttcgct gtcatcaata aacagcgttc tttcagcttt cagacccgta gcttcggcca
    23521 ccgcatgcca taaccgctga tcctctttcg gataaccaaa tgtgtgggtg gaaagtaata
    23581 aatcaaggtg tgcgtccaga ccggtatgct caagttttac cgccaggttg tgcggatgcg
    23641 cattggtgag caaaattcgc tgcttaccgc tggctttcag tgcctcaaga aacggaatgg
    23701 tatcttcacg cagtacggca cgcggtccca tctcggtggt catcgcacag atatccagac
    23761 ccagttgctc actccagtaa tcaagacagt accagtttag cgtatgctgt acgtcgtgat
    23821 attgctggcg catatattcc atcgcttcct gtggcgtaac cccgtttttc gcgccccatg
    23881 tttcaggcac cagcttttgc cagaaatagt tatcgaaggc gaggtcgagc aacgtgccgt
    23941 ccatatccag cagaacggta tctacgtcct gccaggcaat gttgatatgc atgaggaaaa
    24001 tctccagagt gaagcaattt gcgcgacagg gtagcataac ctgccgcgca aacgtgttat
    24061 tcgataaggc tttctgaagg ggtgatcagt tgcgggttca ggcagctttc ataataggcc
    24121 tgaatcttca tcatgcgagt gcgatgacgc tggtaacgcc gccaggcctg tacgccatta
    24181 taaatggcgc tacccagcat cgtcagtagc agcagcgtgg tgctgagata gcgccacagg
    24241 ccggaacgat ccgggatcgg atgaaggcct atatgctgcg taccattggc gtcagtgaag
    24301 atttttgtga cgataccctc ggcgttaaac ggcgtatgca tcagcatttg tgccagtttc
    24361 tggaaagcgt tccactgttc ttgcggcggg tagtcgtaaa gtgatgccga aggccagggc
    24421 tgatcaacaa aatcgctgcc ttcgtcgctg acaatcagga atccgccggg ggccggactg
    24481 tttagtgatt gtgccgctcg cgccgtttca tgcgtgataa acggcgcggt ggaggttgcc
    24541 accaggttat ccagcgattc cgcactcacc gggcgtaata acacattcac gccatcgagc
    24601 ttcccggcgt tggcgcgttt taccagcgcg tcccagtctt tactgttgcc gagattgacc
    24661 agcgcatttt tcagtcgcac acagtcatct ttggcagaac acagatccgc tgtcttcagt
    24721 acaatgtcgc caaaatcatc aagcaatacc atgccggatt tttgaattgc tgaacgtaat
    24781 gaggcgctga cgcgagattc atcttccggt ttagggtgca gctggcgatt aactgcttca
    24841 gtcaatgccg tcgctttgtt gaccagttca gattctggta atggtaatga gcgggcgtcg
    24901 ttccagatga tctgcgagca gtcaaacggt aaaaaaggtg aattggtttt cgcgctccag
    24961 gttccggaag tgcgaatatt acacattccc gtaccgctaa tacgcaatgt atcgcctacc
    25021 cgcacgccag cgtcagccag ttgttttacg ctggtggctt caatggtctg cgcacctttc
    25081 atccatgaga gagtgaattt cagcggcata tccagcggga tccagaataa cagcataaac
    25141 agcaccagca gcgagcccgc agcgataatc gtactgcgca gccagtgctg taacggaaag
    25201 ttttttactt catcatgcag cgaaagatat cgcccctgac gcactaggtg gcggtcgaga
    25261 tagatatcaa tatcggtttg ttgaccgaga tcctgagcaa tgtatggctg ccagtgtgcg
    25321 ggatagacca ggtcgataat accgagtgaa atattgttga tctgttcctg atcgttttcg
    25381 ccaaacaatc cccaacgacg gggtgtaccg cgcagacaat gaatttcccg cagggaggat
    25441 tttgccgggg gggcgaatag cccccacaga cctgcgccca gcagcagtaa cgcgccgcct
    25501 gccagccacg gaacaaatac atccggggta atcaggcaga aaaagaacat caggaaggag
    25561 gcgacgatca gcaacgcttc acgcagcccg cgcggacgac tcagcgcata ttcttcatgc
    25621 gtttctttgc gaatattgag tagctcgatt tgctcactct cttccccgcg aatcgacgcc
    25681 tgcgtggacg gaacaggttg caaggcatag ccgcgagttt cctgcatgta ctcctgcagc
    25741 gtatgaccgt tgagtgaaat aaccagcggc agcgaatcgg tatgaatcag ttcaacggta
    25801 ttctcatcgt tgatgtactg ctcccagaaa gggggcaggt ggacttctac cgaatcgagg
    25861 tagtaacgcc atttattcgg atcgtcggta gagatgccgt aacgcgtgat agcgtgtgtc
    25921 agcatcatga cgttgttgct ttcggcattc agcgccagag agatcggtgc cgcgctggct
    25981 cccgttgggc caggcacctg taatacctgc gtcaggctct caagataatt ttcaacggcg
    26041 ctacgttctt caggtgtgag tttacgcgtt tgcgcatccg cgaaggcgtt ggtccagggc
    26101 agctgacgcc gtctggatcg cacttttatc agccatcccg caagtagtga gcaggccagc
    26161 aaagcagcta aaaaaatcac aatggtgctc atgctttccc catcttactt agtctgtcag
    26221 gcgtggtcag tcgcaccgga cacaatacat gattctgcgg ttggctatcg gcaagcgtgg
    26281 agtttaactt gagcatcatt aagcacatca cagtaaccgc tgatcatagc aaaccctctg
    26341 aaggcggaat atcagcaaat tcctggagtt atttcaacct attgactttt aatttgattt
    26401 gaggacgatt atttattaag ttacataaat gtaaataaca tgcaattcac attgccgaaa
    26461 gcgtgtccga tatcgcacaa taaccgctga tttcacagca tagatcccac gatgagcaaa
    26521 tcattacaaa aacccaccat tctgaatgtt gaaactgtag cccgttcccg actgtttacc
    26581 gtcgagagcg tggatctaga gttcagcaat ggcgtgcggc gtgtttatga acgaatgcgt
    26641 ccaaccaacc gggaagcagt gatgattgtg ccgattgtgg acgatcacct gatcctgatc
    26701 cgcgaatatg cagtgggaac tgaatcctac gaattaggtt tttcgaaagg attaattgat
    26761 ccgggtgaaa gcgtcttcga agccgctaac cgcgagctaa aagaagaggt tggatttggt
    26821 gcgaacgatc tgactttttt gaagaagctc agcatggcac cgtcttactt ttccagcaaa
    26881 atgaatatcg tggtagcgca agatctctac ccggaatcgc tggaaggcga tgagccagag
    26941 ccgctaccac aggtgcgctg gccgctggcg catatgatgg atttgctgga agaccctgac
    27001 ttcaacgaag cgcgtaatgt cagcgcgctg ttcctcgtgc gcgaatggtt gaaagggcag
    27061 gggcgagtgt aaatgcgtga agtttgcctg atgcacaatg cttatcaggc ttatggggtt
    27121 cctgcaatat attgagtttt cacgattttg ttagccggat aacgcgttca cccgtatccg
    27181 gcataaacaa gtgcactttg tcagcaaact gaaaaggcgc cgaagcgcct ttttaatcag
    27241 aacaattcct gtgcctcgcc attatcgata atggtcgttc ccacctcgtg caccgcctgt
    27301 tgtgtcggct gcgtaccttc gatgaaatac tcttcgcggc tgttgccacc attagctaac
    27361 tgcccggtgc tgcgatcgat attcaccgtc acaatacccg gcggcggcgt cagcggctgc
    27421 tccggcacac cttcaagaac ggctttcata taagcgtccc atgcaggctg ggcactcttg
    27481 gcaccgcctt cgtaacctga aatctgatct ttaatcgctc cggaagccgt tgtatgaccg
    27541 agattacgac ggtgatcatc aaagccaatc cagaccgagg tcacaacgcc cggtccgtaa
    27601 cccgagaacc acgcatcttt cgaactgtta gtggtaccag ttttcccgcc gatatcgcga
    27661 cgctgcaaat cacgacctgc acgccagccg gtaccctgcc agcctggctc accaaagata
    27721 ttggtgttca aagcactctt aatcaggaat gccagcggag tgttgatgac gtgcggtgcg
    27781 tactcctgcg cgccagtctt cgccactaac gcctgatttg cctgctccag ctgcggcatt
    27841 ggtacagaaa cattctgctg ctcgcgggag atagcgacat cttcaacatc gttattttcc
    27901 agcacgttcg atttctgcgt atcaccgtaa atcaccggaa tatcgcattc cgggcaggct
    27961 actttcggtt tcgcttcgaa aatcacgccg ccctgatcgt tttcaatttt gctgataaac
    28021 cacgggtcca ccaggaagcc gccgttcgcc atgaccgcgt agccgcgcgc cacctgcatt
    28081 ggggtgaagg acgctgaacc cagcgccagc gattcggtgt ggacaatgtt ttgcgccggg
    28141 aagccgaagc gttgcagata ttctgcagcg tagtcgacgc ccatcgcccg cattgcgcgt
    28201 accatcacca cgtttttcga ctgacccagc ccctgacgta agcgaattgg accggcatac
    28261 tgcggtggtg agtttttcgg ctgccagtca gaaccggcac ctgcgtccca gcgagaaatt
    28321 ggcacatcgt tcaacatact tgccagcgtc agacctttat ccatcgccgc ggtgtagagg
    28381 aacggtttga tgttggaacc cacctgacgc agtgcctggg tggcgcggtt aaacttgctc
    28441 tgattgaaat caaagccacc gaccagcgcc ataacggcac cattttgcgg attgatcgac
    28501 accagtgccg agttcacttc cggcacttgt gccagccacc atgcatcgcc aacctgacga
    28561 acccagattt gctgacccgt ttgcagaaca tcggtcactt tacgcggcgt cggtccctgc
    28621 tgagtatccg aacggtaagg acgcgcccag cgaacgcctt ccatactcaa tgcgacggtc
    28681 gacccgtccg ccagcattgc cgtcgcttcc tgaggattgg cgctggtgac tgcggcaggc
    28741 agcagcggac cataggtcgg cagcgccttc agcgtatcgg taatcttgtt gttatcccac
    28801 gccgactcgc ccactttcca cagcacattt gccgggccgc gatagccgtg gcgcatgtcg
    28861 tagtccagca cgttattacg taccgcctgc tgcgcggcct gctgcacttt gcgggtgatg
    28921 gtggtgtaaa tgcgataacc gtcttcatag gcactttcgc cataacggtt atacatctcc
    28981 tggcgcacca tttcgctcag gtacggcgca gaaaaagcaa tctccggcgc gtgatagtta
    29041 gcgttaatcg cctcagtgcg tgtctgatcg aactgttgct gggtgatata tccttcatcc
    29101 agcatccgcg atagcacgac gttacgccgc gcgacggcac gatccatcga gtagagcggg
    29161 ttgaaggtgg aaggcgcttt cggcagcccg gctatcaccg ccatttcgtt cagcgtcagt
    29221 tggtcgaccg tttttccgaa atagacttgt gccgcagcac cgacaccata ggcgcggtaa
    29281 ccaaggtaaa tcttgttcag ataaagctcg aggatctcgt ctttcgtcag caactgttca
    29341 atgcgaatcg cgaggaagac ttccttaatc ttacgcatca gcgtgcgttc tggactgagg
    29401 aagaagtttc tcgccagctg ctgggtaatg gtacttgccc cttgtgacgc gtggccggag
    29461 aacagcgcca cacttgccgc acggaagatc cccaccgggt caacgccgtg atgctcgtag
    29521 aagcggctgt cttccgtcgc gataaaggct ttcaccatct ccggcgggat ttgatccaac
    29581 gtaaccggaa tacgacgttt ctcaccgtat tgagcaatca gctcgccatc ggcgctgtaa
    29641 atctgcatcg gaatttgcag gcgaacatct tttaatgtcg ccacatccgg cagttgtggc
    29701 tcgatgtagc ggtataggcc ataaatcgag cctgctccca gcagaatgca acagactgca
    29761 aggatcaaaa aatactttac gaacttcact ggaaatttcc catttagttt catttgggca
    29821 gtttataaac aaacgcgcgg tagtataaag gcaagccaga cgcattgata tacccgtcag
    29881 agtgacgggt gataaggaga tcatcacaat ggcatttaag atctggcaaa ttggtttgca
    29941 tttacaacag caagaagcgg tagcggttgc tattgtgcgg ggcgcaaaag aatgcttttt
    30001 gcaacgctgg tggcggttgc cgctggcgca cgacattatc aaagatgggc ggattgttga
    30061 tgcgcagcgg ctggctaaaa cgttgttacc gtggagtcgc gaactgccgc agcgccatca
    30121 catcatgttg gcgtttcccg ccagtcgcac attacagcgg tcatttccgc gcccgtcgat
    30181 gtccctcggt gagcgggagc aaacggcctg gctgtccggg acgatggctc gcgagctgga
    30241 tatggatccg gactccctgc gcttcgatta cagcgaagac tcactcagtc ccgcctataa
    30301 cgtgactgcc gcgcaaagca aagagctggc aacgctgctt actttggcag aaaggttgcg
    30361 tgttcatgtt agtgcgatca ccccggatgc cagtgcatta cagcgattcc tgcctttttt
    30421 accttctcat cagcaatgtc tggcctggcg tgataacgaa cagtggctgt gggcgacgcg
    30481 ctatcgctgg gggcgcaaac tggcggtggg gatgactagc gcgaaggagc tggcggcagc
    30541 gttatccgtt gatcccgaaa gcgtcgcgat atgtggcgaa ggcggatttg atccttggga
    30601 ggccgtttct gttcgtcagc cgccgttacc gccgccaggt ggagactttg ccatcgcgct
    30661 gggctggcgc ttgggaaggc gtactgatga acccgccaat taattttttg ccctggcgac
    30721 agcaacgccg gaccgctttt cttcgtttct ggttgctgat gttcgttgcg cctctgctgc
    30781 tggccgtcgg gataacgcta atactgcgtc tgacaagcag cgccgaagct cgcgtagacg
    30841 ccgttttgct tcagggggaa caacaactcg cccgcagctt acagataacc aaaccacgtt
    30901 tgctggagcg ccaacaaatt cgtgagcagc gtttgcaaag gcagcgccag cgacaattta
    30961 cccgcgactg gcaatctgcg ctggaagcac tggcggcgct tttacccgag cacgcctggc
    31021 tgacaacgat aagctggcag cagggaacgc tggagatcaa ggggcttaca acaagcatta
    31081 ccgcgttaaa cgcactagaa acgtcactcc gccaggatgc atcttttcat ctcaatcagc
    31141 ggggggccac gcagcaggat gcgcagggac gctggcaatt tgagtatcag ttaacaagga
    31201 aggttagcga tgaacatgtt ctttgactgg tggttcgcca catcaccccg cctccgccag
    31261 ttttgctggg cattctggtt gctgatgtta gttacgctca tttttctgtc atcgacacac
    31321 catgaagagc gcgacgcatt aattcgacta cgggcaagtc atcatcagca ttgggccaca
    31381 ctgtatcgcc tggttgacac cactcccttc agcgaagaaa aaacgctgcc cttttcgcca
    31441 ctggattttc agttgcccgg cgcacaactg gttttctggc atccatccgc gcagggaggc
    31501 gagttggcgt tgaaaacgct gtgggaagca gtgccgtcgg catttacacg gctggcagag
    31561 cgcaacgtca gcgtgagccg tttttcatta agcgtggaag gtgatgatct tttgttcacc
    31621 ctacaactgg agacgccgca tgagggttaa acgctggttg ttggcaggta ttgcattgtg
    31681 ccttttaacc ggtatgcgtg acccttttaa accgccggaa gatctatgcc ggattagcga
    31741 acttagccag tggcgctatc aggggatggt agggcgaggc gagcgcatca tcggtgtaat
    31801 aaaagacggg caaaagaaat ggcgacgggt gcagcaaaac gatgtgctgg aaaacggctg
    31861 gacaatttta cagctgacgc tagacgtact aacgctgggt accgggacaa actgcgaacc
    31921 gccacaatgg ttgtggcaac ggcaaggaga tacaaatgaa gcaatggata gccgcactac
    31981 tgttgatgct gatacccggc gtacaggcgg caaagccgca aaaagtgacg ctgatggtgg
    32041 atgacgttcc ggtagctcag gtgttgcagg cgctggctga acaggagaag ttgaacctgg
    32101 tggtgtcgcc agacgtcagc ggtacggtgt cgttacatct aacagatgtt ccctggaagc
    32161 aggcactaca aactgtagtg aaaagcgccg gactgataac gcgccaggag ggcaacattc
    32221 tctcagtgca ttccattgcc tggcagaatg acaatatcgc ccgtcaggaa gcggagcagg
    32281 cgcgggcgca ggcaaatctg ccgctggaaa atcgcaatat tactctgcaa tacgccgacg
    32341 ccggagagct ggcgaaagct ggggagaagc tactgagtgc caaagggagt atgaccgtcg
    32401 ataaacgcac caatcgcctt ttgctacgag ataacaaaac ggcgttaagc gcccttgaac
    32461 agtgggtagc gcaaatggat ctgccggtcg ggcaggttga gctgtcggcg catattgtca
    32521 ccattaatga aaaaagtttg cgtgagttag gcgtgaaatg gacgctggcc gatgcgcaac
    32581 acgctggtgg cgttgggcaa gtcaccacgc ttggtagcga cctctccgta gcgacggcga
    32641 caacgcatgt cggttttaac attgggcgca tcaacggacg tttgctggat cttgagcttt
    32701 ccgcgctcga acaaaaacag cagctggata ttatcgccag tccgcgtctg ctggcctcac
    32761 atcttcagcc tgccagcatt aaacagggga gcgaaattcc atatcaggtt tccagcgggg
    32821 aaagtggcgc gacgtcggtg gaatttaaag aggccgtcct ggggatggag gtcacgccca
    32881 cggtgttaca aaaaggtcgc atccggctga aattacacat cagccagaac gttccggggc
    32941 aggtgctaca gcaggccgat ggcgaagtgc tggcgattga taagcaggag atcgaaacgc
    33001 aggtcgaggt caaaagcgga gaaacgttgg cgctgggcgg catttttacc cgtaaaaata
    33061 aatcgggtca ggatagcgtg ccgttgcttg gcgacattcc ctggttcggg caattatttc
    33121 gtcatgacgg aaaagaagat gaacgacgcg agttagtggt gtttatcacg ccacgactgg
    33181 tttccagtga gtaaacagcc gtaaaagcgg taatgttttt acgctgaacg tgtttcatct
    33241 atttgacgcg cgcaggtatt tagcatacaa ggagtaccga tttgagagtt ggtgctcttc
    33301 gctgcctgcc ttccatgatg atgatttatc attcaggcgg cattttgctg tcttttttac
    33361 gctaatctta cccggtgatt tatcgccaga gcggtggtag caaggcagcg cgcttgcagc
    33421 gaccagatat gcagagggat gggtgattta ttcagttgcc aaacccgctg gagtattgag
    33481 ataattttca gtctgactct cgcaatatct tatgaggttt cagttcatgt cctgcggagc
    33541 tctctgagcg aagcgggttt atcattaacg aatagtctta gtagtaccga aaaaatggca
    33601 gagaaacgca atatctttct ggttgggcct atgggtgccg gaaaaagcac tattgggcgc
    33661 cagttagctc aacaactcaa tatggaattt tacgattccg atcaagagat tgagaaacga
    33721 accggagctg atgtgggctg ggttttcgat ttagaaggcg aagaaggctt ccgcgatcgc
    33781 gaagaaaagg tcatcaatga gttgaccgag aaacagggta ttgtgctggc tactggcggc
    33841 ggctctgtga aatcccgtga aacgcgtaac cgtctttccg ctcgtggcgt tgtcgtttat
    33901 cttgaaacga cgatcgaaaa gcaacttgca cgcacgcagc gtgataaaaa acgcccgttg
    33961 ctgcacgttg aaacaccgcc gcgtgaagtt ctggaagcgt tggccaatga acgcaatccg
    34021 ctgtatgaag agattgccga cgtgaccatt cgtactgatg atcaaagcgc taaagtggtt
    34081 gcaaaccaga ttattcacat gctggaaagc aactaattct ggctttatat acactcgtct
    34141 gcgggtacag taattaaggt ggatgtcgcg ttatggagag gattgtcgtt actctcgggg
    34201 aacgtagtta cccaattacc atcgcatctg gtttgtttaa tgaaccagct tcattcttac
    34261 cgctgaaatc gggcgagcag gtcatgttgg tcaccaacga aaccctggct cctctgtatc
    34321 tcgataaggt ccgcggcgta cttgaacagg cgggtgttaa cgtcgatagc gttatccttc
    34381 ctgacggcga gcagtataaa agcctggctg tactcgatac cgtctttacg gcgttgttac
    34441 aaaagccgca tggtcgcgat actacgctgg tggcgcttgg cggcggcgta gtgggcgatc
    34501 tgaccggctt cgcggcggcg agttatcagc gcggtgttcg tttcattcaa gtcccgacga
    34561 cgttactgtc gcaggtcgat tcctccgttg gcggcaaaac tgcggtcaac catcccctcg
    34621 gtaaaaacat gattggcgcg ttctaccagc ctgcttcagt ggtggtggat ctcgactgtc
    34681 tgaaaacgct tcccccgcgt gagttagcgt cggggctggc agaagtcatc aaatacggca
    34741 ttattcttga cggtacgttt tttaactggc tggaagagaa tctggatgcg ttgttgcgtc
    34801 tggacggtcc ggcaatggcg tactgtattc gccgttgttg tgaactgaag gcagaagttg
    34861 tcgccgccga cgagcgcgaa accgggttac gtgctttact gaatctggga cacacctttg
    34921 gtcatgccat tgaagctgaa atggggtatg gcaattggtt acatggtgaa gcggtcgctg
    34981 cgggtatggt gatggcggcg cggacgtcgg aacgtctcgg gcagtttagt tctgccgaaa
    35041 cgcagcgtat tataactctg ctcacgcggg ctgggttacc ggtcaatggg ccgcgcgaaa
    35101 tgtccgcgca ggcgtattta ccgcatatgc tgcgtgacaa gaaagtcctt gcgggagaga
    35161 tgcgcttaat tcttccgttg gcaattggta agagtgaagt tcgcagcggc gtttcgcacg
    35221 agcttgttct taacgccatt gccgattgtc aatcagcgta acaacaagaa aggtcaggcc
    35281 gcttatcaag cggtctatta gcttcaggtt aattgcaacg tggtaagcat taacctttta
    35341 gtggggtgtt aaatggatga attcaaacca gaagacgagc tgaaacccga tcccagcgat
    35401 cgtcgtactg gtcgttctcg tcaatcttct gaacgttctg agcgtactga acgtggcgaa
    35461 ccgcagatca attttgatga tattgaactt gatgacactg acgatcgccg tccgactcgt
    35521 gcgcaaaaag agcgcaatga ggaaccggaa atcgaagaag aaattgacga atccgaagat
    35581 gaaaccgtgg atgaagagcg cgtagagcgt cgtccgcgta agcgcaaaaa agcagccagt
    35641 aaacccgctt ctcgtcagta tatgatgatg ggcgtcggca ttctggttct actgctgttg
    35701 atcatcggta tcggttctgc gctaaaagcc ccctcgacca cttccagcga tcaaaccgcg
    35761 tctggcgaga agagtattga tcttgctggc aatgcgaccg atcaggcgaa tggcgtgcag
    35821 ccagcgccgg gaaccacgtc tgcggaaaat actcagcagg atgtttctct gccaccgatc
    35881 tcttctacgc cgactcaagg gcaaaccccg gcggcaacgg atggtcaaca acgtgttgaa
    35941 gtgcagggtg acctgaacaa tgcgctgacc cagccacaaa atcagcaaca gttgaacaat
    36001 gtggcggtca attctacatt gccgactgaa ccagcaactg tcgcgccagt acgcaatggc
    36061 aatgcatcgc gtgacacggc gaaaacgcaa actgctgaac gtccggccac tacgcgtcca
    36121 gctcgtcagc aggcggtgat tgaaccgaaa aaaccgcaag caaccgtgaa aacggaaccg
    36181 aagccggtag cacagacgcc gaagcgtact gaaccagctg ctcctgtggc gagcacgaag
    36241 gcaccggctg cgacttctac gccagcacca aaagagacgg cgactacggc tccagtacag
    36301 acggcatccc cggcgcaaac cacggcaaca ccagccgctg gagggaagac cgcaggtaat
    36361 gtgggttcgt tgaaatcggc accgtccagc cattacactc tgcagctgag cagttcctct
    36421 aactacgaca acctgaacgg ttgggcgaag aaagagaatc tgaaaaacta cgttgtctat
    36481 gaaacgacgc gtaatggtca gccgtggtat gtcctggttt ctggcgtgta cgcttcgaaa
    36541 gaagaggcga aaaaagcggt atctacattg ccagcagatg ttcaggccaa aaacccgtgg
    36601 gcgaaaccgc tgcgtcaggt acaggccgat ctgaagtaat caaggttatc tcccgcaatg
    36661 gtttatcgtt gcgggagttg cctgaagcgc tggatgctgt cggagctttc tccacagccg
    36721 gagaaggtgt aattagttag tcagcatgaa gaaaaatcgc gcttttttga agtgggcagg
    36781 gggcaagtat cccctgcttg atgatattaa acggcatttg cccaagggcg aatgtctggt
    36841 tgagcctttt gtaggtgccg ggtcggtgtt tctcaacacc gacttttctc gttatatcct
    36901 tgccgatatc aatagcgacc tgatcagtct ctataacatt gtgaagatgc gtactgatga
    36961 gtacgtacag gccgcacgcg agctgtttgt tcccgaaaca aattgcgccg aggtttacta
    37021 tcagttccgc gaagagttca acaaaagcca ggatccgttc cgtcgggcgg tactgttttt
    37081 atatttgaac cgctacggtt acaacggcct gtgtcgttac aatctgcgcg gtgagtttaa
    37141 tgtgccgttt ggccgctaca aaaaacccta tttcccggaa gcagagttgt atcacttcgc
    37201 tgaaaaagcg cagaatgcct ttttctattg tgagtcttac gccgatagca tggcgcgcgc
    37261 agatgatgca tctgtcgtct attgcgatcc accttatgca ccgctgtctg cgaccgccaa
    37321 ctttacggcg tatcacacaa acagttttac gcttgaacaa caagcgcatc tggcggagat
    37381 cgccgaaggt ctggttgagc gccatattcc tgtgctgatc tccaatcacg atacgatgtt
    37441 aacgcgtgag tggtatcagc gcgcaaaatt gcatgtcgtc aaagttcgac gcagtataag
    37501 cagcaacggc ggcacacgta aaaaggtgga cgaactgctg gctttgtaca aaccaggagt
    37561 cgtttcaccc gcgaaaaaat aattctcaag gagaagcgga tgaaacagta tttgattgcc
    37621 ccctcaattc tgtcggctga ttttgcccgc ctgggtgaag ataccgcaaa agccctggca
    37681 gctggcgctg atgtcgtgca ttttgacgtc atggataacc actacgttcc caatctgacg
    37741 attgggccaa tggtgctgaa atccttgcgt aactacggca ttaccgcccc tatcgacgta
    37801 cacctgatgg tgaaaccggt cgatcgcatt gtgcctgatt tcgctgccgc tggtgccagc
    37861 atcattacct ttcatccaga agcctccgag catgttgacc gcacgctgca actgattaaa
    37921 gaaaatggct gtaaagcggg tctggtattt aacccggcga cacctctgag ctatctggat
    37981 tacgtgatgg ataagctgga tgtgatcctg ctgatgtccg tcaaccctgg tttcggcggt
    38041 caatctttca ttcctcaaac actggataaa ctgcgcgaag tacgtcgtcg tatcgacgag
    38101 tctggctttg acattcgtct ggaagtggac ggtggcgtga aggtgaacaa cattggcgaa
    38161 atcgccgcgg cgggcgcgga tatgttcgtt gccggttcgg caatcttcga ccagccagac
    38221 tacaaaaaag tcattgatga aatgcgcagt gaactggcaa aggtaagtca tgagtaagtt
    38281 tgaagatatt cgcggcgtcg cttttgatct cgacggtacg ctggtggaca gtgctcccgg
    38341 tcttgctgct gcggtagata tggcgctgta tgcgctggag ttgcccgtcg caggtgaaga
    38401 acgcgttatt acctggattg gtaacggcgc agatgttctg atggagcgcg cattgacctg
    38461 ggcgcgtcag gaacgtgcga ctctgcgtaa aacaatgggt aaaccgcccg ttgatgacga
    38521 cattccggca gaagaacagg tacgtattct gcgtaaactg ttcgatcgtt actatggcga
    38581 ggttgccgaa gaggggacgt ttttgttccc gcacgttgcc gatacgttgg gcgcgttgca
    38641 ggctaaaggc ctgccgctag gcctggtcac caacaaaccg acgccgttcg tcgcgccgct
    38701 gctcgaagcc ttagatatcg ccaaatactt cagcgtggtg attggtggtg atgatgtgca
    38761 aaacaaaaaa ccgcatccgg acccgctgtt actggtggct gagcggatgg gaattgctcc
    38821 acaacagatg ctgtttgttg gcgactcacg caatgatatt caggcagcaa aagcggcagg
    38881 ttgcccatca gttggcttaa cctacggata taactacggc gaggctatcg atctcagcca
    38941 gcctgatgta atttatcagt ctataaatga ccttctgccc gcattagggc ttccgcatag
    39001 cgaaaatcag gaatcgaaaa atgactaagc ccatcgtttt tagtggcgca cagccctcag
    39061 gtgaattgac tattggtaac tacatgggtg cgctgcgtca gtgggtaaac atgcaggatg
    39121 actaccattg catttactgt atcgttgacc aacacgcgat caccgtgcgc caggatgcac
    39181 agaagctgcg taaagcgacg ctggatacgc tggccttgta tctggcttgt ggtatcgatc
    39241 ctgagaaaag caccattttt gttcagtccc acgtaccgga acatgcgcag ttaggctggg
    39301 cactgaactg ctatacctac ttcggcgaac tgagccgcat gacgcagttt aaagataaat
    39361 ctgcgcgtta tgccgagaac atcaacgctg gtctgtttga ctatccggtg ctgatggcag
    39421 cggacatcct gctgtatcaa actaatctgg taccggtggg tgaagaccag aaacagcacc
    39481 tggaactgag ccgtgatatc gcccagcgtt tcaacgcgct gtacggcgat atctttaagg
    39541 tgccggagcc gtttattccg aaatccggcg cgcgcgtaat gtcgctgctg gagccgacca
    39601 agaagatgtc caagtctgac gataaccgca ataacgttat tggcctgctg gaagatccga
    39661 aatcggtagt gaagaaaatc aaacgcgcgg tcaccgactc cgacgagccg ccggtagttc
    39721 gctacgatgt gcagaacaaa gcgggcgttt ccaacctgct ggatatcctt tctgcggtaa
    39781 cgggccagag catcccggaa ctggaaaaac agttcgaagg caagatgtat ggtcatctga
    39841 aaggcgaagt ggctgatgcc gtttccggta tgctgactga attgcaggaa cgttaccacc
    39901 gtttccgcaa cgatgaagcc ttcctgcaac aggtgatgaa agacggcgca gaaaaagcca
    39961 gcgcgcacgc ttcccgtacg ctaaaagcgg tgtacgaagc gattggtttt gtggcgaagc
    40021 cgtaatatca aaacgctcgg acggtcccct cgcccctttg gggagagggt tagggtgagg
    40081 ggaacgtgcg ggatgtaaaa ttcagtgcgc cggcatcgcc ggatgcgatg ctggtgcatc
    40141 ttatccggcc tacggttaat gtcttttatt atcaattgtt aaggtgatgc ggttggcgcg
    40201 gcagaagagt tcggagacgt gcaccggttt gtcattctga tcaaaggcga ttttgctgat
    40261 gcgaaacaac ggttcgccca gttcgcattt cagccatttt gcctgcgggc gggtggcggc
    40321 aaagatatcg atggtctttt tgtcgctgac cactcgcgtg tcaaaacgct cctgaaataa
    40381 ctgataggtg gagcttcctt cgacgtaaat ctcatcaaag tcaggataac gcgacagcgg
    40441 gatccacgaa ctatcaataa atagcggctc tttatcgaga tacatcaccc ggcaaagatg
    40501 gaacacttcg ctgttaccgg ggatattgag cttttcgcaa aacggcgcgg cgctgatccg
    40561 ttcttgctcg atcactttct ctttcgtcgc cttgccttgt gagacgccaa aatcggtaaa
    40621 accactgacg gtcagcaggg cgttttcgac cttctggctt tgtacaaagg tgcctttgcc
    40681 ctgccagcgg atcagtacgc cgtctgccac taagtcgctg atggctttgc gaatggtaat
    40741 gcggctgacg ttatattgtg tacaaagctc attttcggta gggatctgtt gcccggcctg
    40801 gtaaaccccc tgcgcgatat catccagcag ccgctggcgg acggtagcgt agaggagttg
    40861 atgagagtag cggtccgtag ctgacataat cgaccctgaa ataaagaatg aattacacgg
    40921 attatagggt cgttttccgg caagtaacag cacgttggcg tgaagatgct cacgccaacg
    40981 ttatgcctac caggcaccgt ggtactgaat agtttgtgcc gcgcacgccg ttccctgcgc
    41041 catcgcctgc ggtaatgtca tccccgcaga ccagccgcaa aggaatccgg caatgaacga
    41101 atctccggca cccatggtgt cgataaccgt cacctgttca ggagcctgac gccagaactg
    41161 cacgccatcc caggcaatac tgccgttttc acccaacgtg acaatcacca cgcctgcgcc
    41221 acgggcaaca atcgctttca tcttcagacg cagcgcttcg tcttcttgcg gtgcggaggc
    41281 aaaggcaaaa tcgagatgcg gcaccagtgt ctgccagagc gggctgtccc acttgtcgga
    41341 gacgtcgaaa gcggtaagtt tgcccgcagc gtgcagctgt gggaatgcgt cttccgcatg
    41401 tccccagatt gccgcgtgca caatgtcata ctgcgccagc taggcgtaat cctcttcact
    41461 cagggcaaag tcggccatca cgccttcggt gtagtcgcca aaaacgcgat cattgtcgtg
    41521 cagttccacc tgagtttgtg cggtaacgcc gtgtttcgta tggacatggc tgatatcgac
    41581 gcccatgctg gcgagatcct gcttaagctt tgtgccgtag tcatcgtcac caacccaggt
    41641 aatgcatccc ggctttatac cgtagcgagt gcagtacacc gccacgttca ccgcattacc
    41701 gccagaaaac gctttattca gttgcgggta gatatcgacg cagttatcgc cgattgtcgc
    41761 cagggttttc atcactcatc ctccggcagc agcgcgcgaa agcgttccag cgcctggcgg
    41821 gcgtaaagtc tgggctcgtt catatacatg gttaccagtt ccactgtgca gtatccctca
    41881 tagccccgat caataatatc gcgcatcagt tcccgcagcg gcattttgcc ttcgccggga
    41941 atgtaatgcg tgtcgctggc cccgtcgctg tcgacaatat gcagatgacg taatttatcg
    42001 cccagtttgt cgaaataact catcaccggt tccgcctgga catacggcgc gcaaatgtcg
    42061 accatgctga acaggcgcgg cgaaggcacc agcgccagcg catgaagcac atcattagca
    42121 ttacacacga cgttcgattc atacggcgtt aacggttcga ggatcagatc catgccgatg
    42181 ttttccgcgt actcacacag ttcgcttaag ttctctgcca gccgccccca gataacgtta
    42241 ggcggcgtga gatagcccgc gtgggccgca gaaatcagcg tataacccgc gttcatctct
    42301 tttgccatat ccatcgccag cttgatcatg tccaggcttt cacgacgcat atgttcatcg
    42361 cccagcatca tgttatacgg atagccgttg gtttctggcg tgtaaccgat aatcggcatc
    42421 tgatacgtct gcgccagcgc cttgatttgt ttgatgccgc ccgcttttaa gtccggcgcg
    42481 aacgcgtgcg ggcggccgcc ccaaatttcg atgccatcgt aacccagctc gcttgcatca
    42541 cggaatgcat gttcaatagg cagccgctgg tggccgcagg taaacatacc tgttttcatc
    42601 gggtaactcc ttgcgtaaat ccggcccgtt gccgggccgg gagagaggat taatattcca
    42661 ccagaccacc gtaatagcgg cgttcatccg ggttgtgatc tttgtaaata gacaggtagt
    42721 agcagagcca ctccattggc acgaacatca ggaacggtgc cagccacggg tgcagtcctt
    42781 gcgaaattgc ggcgtaatcg atgacgatca cgttgtcagt acgctgttta acaaagttaa
    42841 tggcgcgttc ggtggtgtgg cgactttcat cattgccgag caggaacagg aacggaacgc
    42901 ccggttcgac aatctccagc gggccgtgac ggaactcacc gctctcaatc acacagccgt
    42961 gcgtccaggt aaattccatc agcgtcacga tgccttcttt gtaacccagc gggcgcagcg
    43021 ggcctgcagc aaccgtgtaa atcatcggcc actggctggc cagttcacca agctggcggc
    43081 ctttttcttc ccaagtgcgt accagataac cgagcgcatt cggcagctgt ttgagatcgt
    43141 ttttgatctt gccgatttcc gcgtgcggcg cgaggcgggt gatcatctcc agcaccacgc
    43201 tgtagcagag cagcagatga atttcccaga tacagtcggc ctgataatca atgctaaatt
    43261 ccgccgccga ggtaatcggg ctatccgcgc gtttggtgaa cgccgcagtg agtgcgccgc
    43321 aggcccggcc cagctccagc gctttgatta cctcttcggt tttaccgtag tcagaaacgc
    43381 caatcaccgc gcaacgagcg tcgaggcgat atggggtgtt atcgcagaac tcccagccgg
    43441 aaatggcgta aacctgaaga tcggaaaagc gatctgccag atgtttcgcc gtttgcgccg
    43501 cgttgagtgg cgatccgcag gcaacgaaat aaatacgatc gatgtcgcgc ttcaccatct
    43561 cttccacgat ggtgtgcacc agcggaacgt catggctgag aactttttcc acttcctgaa
    43621 ccatattttc ggtgaccaga aagtccacag tgcttttatc aatatccaac attttatttt
    43681 ctccagataa cattacgaca aagcgttgag ctggcggcta cgcttcgccc agaaggcgta
    43741 agcaggcaga ccagtggcaa taacgatgac ggcgcagata aggccgggaa tcggtgccca
    43801 gacaaaggtt gaggcgacca gaatgaggct tgacgcaatg gcgagggggg tcatcagccc
    43861 gaaagccgga gtacgccaca gcggtttgta atcgtcgcgt ttacgacacc agatgatgga
    43921 gccgaaggtg agggtatttt tgaaacacat taccagggtg aaatagccca gcaggctggt
    43981 gagatcggaa acgaagatga agaagatccc cagcgcccct tgcaaaatga tggagacatc
    44041 cggcgtgttg tatttcggat gcacatggcc gaagcatttg aagaacaggt tgtctttcgc
    44101 catcgcgtat tccagccgcg gctggtacat cacgcagctg gaaagcgaac caagaatgac
    44161 gatcatcgcc gtgatggcaa caaagatccc agcggtgctg ccgagtgcgg ggatccaggt
    44221 cagggcgtcg gaaatcggcg tttcagaatt ggcgagtttg tcgaagggca tcaggccgga
    44281 aatcaccagc gccagcaggg tgtagagcac cagaaccagc agacaggaac caatcagcgc
    44341 tcgtggcatg gttttgccgg ggtttttaat ttcgccggtc atataacaga tagaggccat
    44401 gccggtatac gaccaactgg tggcagagat ccccgccagc agcgccataa agctgcccgt
    44461 tgcgccaatc gcagtggtag caggggcggc aaaattctcc gctttaaacc agaagatccc
    44521 aagaccaatg acgatagtga acgggataat tttggcgatg gtaattagcg tctgaaacgc
    44581 tgcgccgcct tcaacggagc gcaggtgcag cagcataaag gcgataatta atccggcggc
    44641 gataaattta ccgagcaacg gatcgatagg cgttaaaaag ccaagattgc tgacaatcgc
    44701 cagcgccata atcgacaatg acggcgcatc gttggcccag aagctggccc agccggagag
    44761 gaaagccagc ggtcggcttc cggcattttt cagataaaca taatctgcgc cattttccgg
    44821 ataagcggtg gatagttccg catatacgca catttgcggg atcacaatta acccgccgat
    44881 gacaaatgcg agcaccgtca gccacggcgt gcccgccgct tttgccactt cacccacaga
    44941 tacaaaaata ccggagccga cggttgtccc gacggcgatt gcaagaacgg cccaaaatcc
    45001 gagcttgcgt tggagttcct ggcttcccat agcgatacct ttttcttgtc caacacgttg
    45061 taggggagag tttgcagtca tttaaaaggt gacatgacaa tgcatgatga atataacaac
    45121 atatgatgtt atgtaatgtg gaggaagatc acataatttc gcacaggatc gcgctgtggc
    45181 tgatgaatgt ggttatccaa ttgaaattaa agtgaaaata tttttacggg tggggagcaa
    45241 gaaggatata gaaacaaata cgccctcggg taatcccgag ggcgtcaggc aattaaatca
    45301 gcgttagctg atttctgtca gagacttact gtgcagtagg accgcaaccg ccaatgattt
    45361 tagaaataga aattgctggg tgcagcaggt agtcgtaaga gcagttctta tcacggtttt
    45421 caatatgacc agtacatgca gttaacgtag ccagtactgc acctaccagt gcaactttaa
    45481 ta
//
LOCUS       DBFIFI010000017        45011 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.017, whole genome shotgun sequence.
ACCESSION   DBFIFI010000017 DBFIFI010000000
VERSION     DBFIFI010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45011
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.017"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(85..1734)
                     /gene="actP"
                     /locus_tag="N7B39_000940"
     CDS             complement(85..1734)
                     /gene="actP"
                     /locus_tag="N7B39_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HGU2420964.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRNDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSITVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1731..2045)
                     /gene="yjcH"
                     /locus_tag="N7B39_000941"
     CDS             complement(1731..2045)
                     /gene="yjcH"
                     /locus_tag="N7B39_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HGU2420965.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPVGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2246..4204)
                     /gene="acs"
                     /locus_tag="N7B39_000942"
     CDS             complement(2246..4204)
                     /gene="acs"
                     /locus_tag="N7B39_000942"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HGU2420966.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINAPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLKLGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDQVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDS
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4597..6033
                     /gene="nrfA"
                     /locus_tag="N7B39_000943"
     CDS             4597..6033
                     /gene="nrfA"
                     /locus_tag="N7B39_000943"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HGU2420967.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSHDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6078..6644
                     /gene="nrfB"
                     /locus_tag="N7B39_000944"
     CDS             6078..6644
                     /gene="nrfB"
                     /locus_tag="N7B39_000944"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HGU2420968.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6641..7312
                     /gene="nrfC"
                     /locus_tag="N7B39_000945"
     CDS             6641..7312
                     /gene="nrfC"
                     /locus_tag="N7B39_000945"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HGU2420969.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7309..8265
                     /gene="nrfD"
                     /locus_tag="N7B39_000946"
     CDS             7309..8265
                     /gene="nrfD"
                     /locus_tag="N7B39_000946"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HGU2420970.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLSLVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAQFVH
                     RMEIPVVWGEIFLLAAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LFKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8285..10003
                     /gene="nrfE"
                     /locus_tag="N7B39_000947"
     CDS             8285..10003
                     /gene="nrfE"
                     /locus_tag="N7B39_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HGU2420971.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWKLKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWQYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRAQLPALLAHAGILLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDV"
     gene            9996..10379
                     /gene="nrfF"
                     /locus_tag="N7B39_000948"
     CDS             9996..10379
                     /gene="nrfF"
                     /locus_tag="N7B39_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HGU2420972.1"
                     /translation="MYKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKSEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10376..10972
                     /gene="nrfG"
                     /locus_tag="N7B39_000949"
     CDS             10376..10972
                     /gene="nrfG"
                     /locus_tag="N7B39_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HGU2420973.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCIGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQNPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11314..12627
                     /gene="gltP"
                     /locus_tag="N7B39_000950"
     CDS             11314..12627
                     /gene="gltP"
                     /locus_tag="N7B39_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HGU2420974.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12805..13494)
                     /gene="yjcO"
                     /locus_tag="N7B39_000951"
     CDS             complement(12805..13494)
                     /gene="yjcO"
                     /locus_tag="N7B39_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HGU2420975.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13588..15735)
                     /gene="fdhF"
                     /locus_tag="N7B39_000952"
     CDS             complement(13588..15735)
                     /gene="fdhF"
                     /locus_tag="N7B39_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(15316..15318),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase N subunit alpha,
                     selenocysteine-containing"
                     /protein_id="HGU2420976.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLSIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15933..17399)
                     /gene="mdtP"
                     /locus_tag="N7B39_000953"
     CDS             complement(15933..17399)
                     /gene="mdtP"
                     /locus_tag="N7B39_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HGU2420977.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVTAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDSIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGSGYQAAPVVEKK"
     gene            complement(17396..19447)
                     /gene="mdtO"
                     /locus_tag="N7B39_000954"
     CDS             complement(17396..19447)
                     /gene="mdtO"
                     /locus_tag="N7B39_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HGU2420978.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAITEGQCWQSDWRISESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIHQGRDLLHAWDATWNSAQALDNALQPDKAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19447..20478)
                     /gene="mdtN"
                     /locus_tag="N7B39_000955"
     CDS             complement(19447..20478)
                     /gene="mdtN"
                     /locus_tag="N7B39_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HGU2420979.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYV
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKSEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20497..20772)
                     /gene="ytcA"
                     /locus_tag="N7B39_000956"
     CDS             complement(20497..20772)
                     /gene="ytcA"
                     /locus_tag="N7B39_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HGU2420980.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRVIQRANIKLAFVGIIYTALFALYAMLFWLAFF"
     gene            complement(20981..22966)
                     /gene="yjcS"
                     /locus_tag="N7B39_000957"
     CDS             complement(20981..22966)
                     /gene="yjcS"
                     /locus_tag="N7B39_000957"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HGU2420981.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKVYYRADDYKFDINAAAPETV
                     NPSLWRQSQINGISGLFKVTDKMYQVRGQDISNITFVEGEKGIIVIDPLVTPPAAKAA
                     LDLYFQHRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLPHNAQGNVGNGLGVTLATGDPSIIAPTKTIVRTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTITFIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVKALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMAVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQPQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
     gene            23257..23964
                     /locus_tag="N7B39_000958"
     CDS             23257..23964
                     /locus_tag="N7B39_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGU2420982.1"
                     /translation="MKPVVLVVDDDTAICALLQDVLSEHVFTVSVCHTGQEAILRIEG
                     DPDIALVVLDMMLPDTNGLRVLQQIQKLRPTLPVVMLTGMGSESDVVVGLEMGADDYI
                     CKPFTPRVVVARLKAVLRRVGALAVNDEKSAGLSFNGWYLDTMRCQLHNPLQQHVELT
                     QGEYGLLLALAQNARRVLSREQLLAFTHSDSVEVFDRTIDVLIMRLRRKIELNPHQPM
                     LIKTLRGLGYVFAADVH"
     gene            complement(23961..24962)
                     /locus_tag="N7B39_000959"
     CDS             complement(23961..24962)
                     /locus_tag="N7B39_000959"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HGU2420983.1"
                     /translation="MMERKGIIAAGNMLVDHVHQIIQWPERGWLAEITHSERSTGGAP
                     LNVLLTLAKMRTGLPLQAVGLIGQDSDGDYIMAMLEQYHVNRQHVQRTTFAPTSMSQV
                     MTDPSGQRTFFHSPGANRLLDLPAFDQLDNTMKIFHLGYLLLLDSLDMPDDVYGTRSA
                     RLLAQMRDTGFETSLDLVSRKGDPRYQPLVLPALRHLDYLVINELEAGEFSGLEIRLP
                     NGEPHITHIAAAARELLDAGVRQRVVIHYPEGAWGETPEQGGVWIPSQKLEQREIIGS
                     VGAGDAFCAGFLYGCHERWTLTESIKLAHACARASLLCANAIDGAKTLEELQTEMSD"
     gene            complement(24959..25819)
                     /locus_tag="N7B39_000960"
     CDS             complement(24959..25819)
                     /locus_tag="N7B39_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose 1,6-bisphosphate aldolase"
                     /protein_id="HGU2420984.1"
                     /translation="MPLISLAEGLAHAREHHYSLGAFNVLDSHFLRALFAAARQERSP
                     FIINIAEVHFKYISLDSLVETVKFEAARHDIPVVLNLDHGLHFESVVQALRLGFSSVM
                     FDGSTLGYEENVRQTREVVKMCHAVGVSVEAELGAVGGDEGGALYGHADESLFTDPQL
                     ARDFVDRTGIDALAVAIGNAHGKYRGEPKLDFPRLDAIRQQAAIPLVLHGGSGISDAD
                     FRRAIELGIHKINFYTGMSQAALAAVEARMAHRQPMYDEFAELLLAVEAAISDTVAQQ
                     MRIFGSAGQA"
     gene            complement(25834..26517)
                     /locus_tag="N7B39_000961"
     CDS             complement(25834..26517)
                     /locus_tag="N7B39_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HGU2420985.1"
                     /translation="MKRSAINDILGHTRQFFSQHDVHLPPFASFSPAQWQQLDTAAWE
                     EVFDLKLGWDVTAFGRNNFAAHGLTLFTLRNGSAKGMPYVKCYAEKIMHVRDAQVTPM
                     HFHWRKREDIINRGGGNLIVELWNADSNEQTADSDITVVIDGCRQKHTAGSQLRLSPG
                     ESICLPPGLYHSFWAEAGFGDVLVGEVSSVNDDDHDNHFLQPLDRYNLIDEDEPAQLV
                     LCNEYRQFR"
     gene            complement(26572..27513)
                     /locus_tag="N7B39_000962"
     CDS             complement(26572..27513)
                     /locus_tag="N7B39_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGU2420986.1"
                     /translation="MRLKPLVTALCAGALLAAAPFAQAKELKSIGVTVGDLANPFFVQ
                     ITKGAELEARKLAGDNVKVTLVSSGYDLGQQVSQIDNFIAANVDMIILNAADSKGIGP
                     AVKRAKDAGIVVVAVDVAAEGADATITSDNTQAGEMACKYITDRLKGKGNVVIINGPP
                     VSAVQNRVEGCQTEFKRHPDIKVLSDNQNAKGSREGGLEVMTGLLAANPKIDGVFAIN
                     DPTAIGADLAAKQAQRNEFFIVGVDGSPDAEEALKRENSLFVATPAQDPQVMAAKAVE
                     IGYDILQGKPAPKEPVLIPVTMIDKNNVGNYKGWTVK"
     gene            complement(27532..28506)
                     /locus_tag="N7B39_000963"
     CDS             complement(27532..28506)
                     /locus_tag="N7B39_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HGU2420987.1"
                     /translation="MTTSAKTSAKKMLMSDLMQTVGILPILILIVAVFGFITPNFFTE
                     SNLLNITRQASINIVLAAGMTFIILTGGIDLSVGSILGTTAVAAMVVSLSPEFAMLSI
                     PAALMLGLLLGLFNGALVAFAGLPPFIVTLGTYTALRGAAYLLADGTTVINSNINFEW
                     IGNNYLGPVPWLVVIALAVIVVCWFILRRTTLGVHIYAVGSNMQAARLTGIKVWLVLL
                     FVYGMSGLLSGLGGIMSASRLYSANGNLGMGYELDAIAAVILGGTSFVGGIGTITGTL
                     VGALIIATLNNGMTLMGVSYFWQLVIKGAMIIIAVLIDKYRTRHHQSA"
     gene            complement(28503..30023)
                     /locus_tag="N7B39_000964"
     CDS             complement(28503..30023)
                     /locus_tag="N7B39_000964"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HGU2420988.1"
                     /translation="MNSTPVLEMRNIAKAFGKFYALKGVDLTVYPGEIHALMGENGAG
                     KSTLMKVLAGAYTATSGEILIDGKPFHIRTPKDALSAGITLIYQEMQLAPNLSVAENI
                     FLGSELSHGGLVQRKEMLVQAQKVIDRLGAQFNASDKVMTLTIAEQQQVEIARALHRN
                     SRILVMDEPTAALSSRETHRLFELIMRLRDEGMAIIYISHRMAEVYELSDRVSVLRDG
                     QYVGSLTRDNLNAGELVRMMVGRPLSDLFNKERDIPLGKARLNVHHLTDGGKVQPSSL
                     LVRSGEIVGLAGLVGAGRSELAQLIFGVRKATGGMIEVDGEPVVIHSPREAIDLGIGF
                     LTENRKEQGLFLEMAAAENITMATLERDARWGMLNRKKAQTISDDAIKLLNIRVPHAQ
                     VRAGGLSGGNQQKLLISRWVAIGPRILLLDEPARGVDVGAKSEIYRIMNEMARKGVAI
                     LMISSELPEIVGMSDRVYVMREGSIAGELNGKNITQENIMTLATGVNDAHSQAVTS"
     gene            30137..32407
                     /locus_tag="N7B39_000965"
     CDS             30137..32407
                     /locus_tag="N7B39_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGU2420989.1"
                     /translation="MAHPGRHFFASARGRLLLLNLLVVAVTLMVSGVAVMGFRHASQM
                     QELVQQQTVDDMTGSLNLARDTANVATAAVRLSQVVGALEYKGEAERLQETQRALKSS
                     LAQLANAPLAQQEAGLVTRIITRSNELQTSVGGMLERGQRRHLERNALLSSLYQNLSY
                     LRHLQKVTHAQDDILLNEMNRLIVAAIATPAPQAIIHQLVGVMSALPTHSDTPLVNTL
                     LNDFNDFNDEMRKLAPLSAALEQSDLAISWYMFHIKALVAILNGDINRYAEQVATLSG
                     QRVAQSYQDLRSGERVIMIFALLAVVITAFAGWYIYRNLGTNLTAISRAMSRLAHGEA
                     DVTFPALQRRDELGELARAFNVFARNTASLERTSRLLKEKTTQMEIAQTERQGLEEAL
                     LHSQKLKAVGQLTGGLAHDFNNLLAVIIGSLELVNPDSPDAPRIQRALQAAERGALLT
                     QRLLAFSRKQSLHPQAVELKTLLEELSELMRHSLPATLALEIEAQSPAWPAWIDISQL
                     ENAIINLVMNARDAMEGQTGIIKIRTWNQRVTRSSGQRQDMVALEVIDHGCGMSQAVK
                     SQVFEPFFTTKQTGSGSGLGLSMVYGFVRQSGGRVEIESAPGQGTTVRLQLPRALTAA
                     KNLSPAAVEQASVSGDKLVLVLEDEAAVRQTICEQLHLLGYLTLEASSGEQALDLLAA
                     SAEIDIFISDLMLPGGMSGAEVVNAARKLYPHLTLLLISGQDLRPSHNPALPDVALLR
                     KPFTRAQLAQALGQEN"
     gene            32476..32805
                     /gene="yjdP"
                     /locus_tag="N7B39_000966"
     CDS             32476..32805
                     /gene="yjdP"
                     /locus_tag="N7B39_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HGU2420990.1"
                     /translation="MKRHSTLFLFTLLTLTTVPAQADIIDDTIGNIQQAINDASNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQHQLNQERRQLEDEERR
                     MEDEYGQ"
     gene            complement(32882..33640)
                     /gene="phnP"
                     /locus_tag="N7B39_000967"
     CDS             complement(32882..33640)
                     /gene="phnP"
                     /locus_tag="N7B39_000967"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HGU2420991.1"
                     /translation="MSLTLTLTGSGGAQGVPAWGCECAACARARRSPHYRRQPCSGVV
                     KFNEAITLIDAGRHDLADRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPREDAPRNHCDLNTVLALNQVIRSPRV
                     VLTHISHQFDAWLMENALPSGFEAGFDGMEIGVA"
     gene            complement(33642..34077)
                     /gene="phnO"
                     /locus_tag="N7B39_000968"
                     /pseudo
     CDS             complement(33642..34077)
                     /gene="phnO"
                     /locus_tag="N7B39_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
     gene            complement(34064..34621)
                     /gene="phnN"
                     /locus_tag="N7B39_000969"
     CDS             complement(34064..34621)
                     /gene="phnN"
                     /locus_tag="N7B39_000969"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HGU2420992.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYQSALLPICLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            complement(34621..35757)
                     /gene="phnM"
                     /locus_tag="N7B39_000970"
     CDS             complement(34621..35757)
                     /gene="phnM"
                     /locus_tag="N7B39_000970"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HGU2420993.1"
                     /translation="MIINNVKLVLENEVVNGSLEMQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHNTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAM
                     CRARQIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDQSNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            complement(35754..36434)
                     /gene="phnL"
                     /locus_tag="N7B39_000971"
     CDS             complement(35754..36434)
                     /gene="phnL"
                     /locus_tag="N7B39_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HGU2420994.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWRLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGAAIVGIFHDEAVRNDVADR
                     LHPMGASS"
     gene            complement(36545..37303)
                     /gene="phnK"
                     /locus_tag="N7B39_000972"
     CDS             complement(36545..37303)
                     /gene="phnK"
                     /locus_tag="N7B39_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HGU2420995.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYGMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            complement(37300..38145)
                     /gene="phnJ"
                     /locus_tag="N7B39_000973"
     CDS             complement(37300..38145)
                     /gene="phnJ"
                     /locus_tag="N7B39_000973"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HGU2420996.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     VIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            complement(38138..39202)
                     /gene="phnI"
                     /locus_tag="N7B39_000974"
     CDS             complement(38138..39202)
                     /gene="phnI"
                     /locus_tag="N7B39_000974"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HGU2420997.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDESPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            complement(39202..39786)
                     /gene="phnH"
                     /locus_tag="N7B39_000975"
     CDS             complement(39202..39786)
                     /gene="phnH"
                     /locus_tag="N7B39_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HGU2420998.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVSQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAVPRTTHVEVC"
     gene            complement(39783..40235)
                     /gene="phnG"
                     /locus_tag="N7B39_000976"
     CDS             complement(39783..40235)
                     /gene="phnG"
                     /locus_tag="N7B39_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HGU2420999.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITANYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQTRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            complement(40236..40961)
                     /gene="phnF"
                     /locus_tag="N7B39_000977"
     CDS             complement(40236..40961)
                     /gene="phnF"
                     /locus_tag="N7B39_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HGU2421000.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGIALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            complement(40982..41761)
                     /gene="phnE"
                     /locus_tag="N7B39_000978"
     CDS             complement(40982..41761)
                     /gene="phnE"
                     /locus_tag="N7B39_000978"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HGU2421001.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMLVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            complement(41867..42883)
                     /gene="phnD"
                     /locus_tag="N7B39_000979"
     CDS             complement(41867..42883)
                     /gene="phnD"
                     /locus_tag="N7B39_000979"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU2421002.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFSKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAMQ"
     gene            complement(42908..43696)
                     /gene="phnC"
                     /locus_tag="N7B39_000980"
     CDS             complement(42908..43696)
                     /gene="phnC"
                     /locus_tag="N7B39_000980"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HGU2421003.1"
                     /translation="MQTIICVEQLSKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMAHFAYQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRVEENAKAA"
     gene            complement(43829..44272)
                     /gene="yjdN"
                     /locus_tag="N7B39_000981"
     CDS             complement(43829..44272)
                     /gene="yjdN"
                     /locus_tag="N7B39_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HGU2421004.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            complement(44531..44866)
                     /gene="yjdM"
                     /locus_tag="N7B39_000982"
     CDS             complement(44531..44866)
                     /gene="yjdM"
                     /locus_tag="N7B39_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HGU2421005.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
BASE COUNT         9681 a        12267 c        12732 g        10331 t
ORIGIN      
        1 tatttgttta atattatggg aaaaggtgat gcatttggga gaggaagagt attccccggt
       61 cagacgaccg gggaagggga gagattaatg cgcgcggcct tgctcaacgc caaagccggt
      121 ctgggagcgg ataaactgcg cgcggaacag ttcacgctca cgcgcgcctt ccgctgagtt
      181 atcggttgcc gagaagaacc agatgccgag gaatgccacg gtgatagaga acagcgccgg
      241 gtattcatac gggaagatgg ctttttcgtg accgaggatc tgtacccaaa tcgtcgggcc
      301 gaggatcatc agtaccactg cggtaatcag ccccagccag ccacccatca tcgcgccacg
      361 cgtggtcagt ttcgaccagt acatagaaag cagaatgatc gggaagttac agctcgccgc
      421 gatggcaaac gccagcccca ccataaaggc gatgttctga ttctcaaaca gcacgccgag
      481 gataatcgca atcacaccca aaatcaggac ggtgattttc gatacccgca gctcttcacg
      541 ttcggtcgcg ccttttttga agacgttagc gtacaggtca tgcgaaaccg ctgatgcgcc
      601 cgccagcgtc agacccgcaa ccaccgcgag gatagtggcg aaagcaaccg ctgaaataaa
      661 gccgaggaac aggttgccgc ccaccgcatt ggccaggtga actgctgcca tgttgttacc
      721 accaatcaga tggcccgccg cgtctttata ttccggattc gcaccgacca gcatgatcgc
      781 gccgaagccg ataataaagg tcaggatata gaagtagccc ataaatccgg tagcgtagaa
      841 cacgctttta cgcgcttcgc gggcatcgct gaccgtaaag aagcgcatca gaatgtgcgg
      901 caagccagcc gtaccaaaca tcagtcctag ccccagagag agtgcggaga tcggatcttt
      961 caccagtccg cccggtttca tgatgtcgac acctttcggg tgtaccgcca tcgcttcgct
     1021 gaacagattg ttgaagctaa agccgacgtg tttcatcacc ataaaggcca taaagctggc
     1081 accgaacagc aacagcacag ctttgataat ctgtacccag gtggtcgcca gcatgccgcc
     1141 gaacaggacg tacatcatca tcagcacgcc gaccagcacc actgcaatgt gatagttaag
     1201 gccaaacagc agctcgatca gtttacctgc ccccaccatc tgggcgataa ggtaaagcgc
     1261 caccaccacc agagaaccac aggccgaaag aatacgaatc ggcccttgtt tcagacgata
     1321 agaggctaca tcggcaaagg tgtaacgccc caggttacgc agacgttcgg caatcaggaa
     1381 caaaatgatc ggccagccca ccaggaagcc cagcgagtaa attaagccgt catagccgga
     1441 ggtaaacacc agtgcggaga tccccaaaaa tgaggcggcg gacatatagt caccggcaat
     1501 cgccagcccg ttctgaaaac cggtgatatt gccgcctgcg gtgtaatagt cattacgcga
     1561 acgtacgcgt tttgacgccc agtaggtaat gccgagcgta aacacgacga aaatcaggaa
     1621 cataataatc gcctgccagt tcgttggctg gcgctctacg gccccgctaa tagcatccgc
     1681 ggcgttagct gcgaaaggga gtgtggcggc aagcgccgtc agaactcttt tcatgatgct
     1741 tgtacctcat gcaggacttc attattaaga cggtcgaatt cgccgttcgc ccgccagatg
     1801 tagataccag tgagaacaaa ggagatcact attaccccaa caccgactgg aataccgcgt
     1861 gtgacgctgg tgttcggatt cagcggggtg cccagccagc cgggcgcgaa ggcgatcagt
     1921 aaaataaagc cgatataaac tgccagcata ataatcgaca ggatggtggc aaaccgttgc
     1981 cgtttttcga ctaactccct gaaatgcgca ttgtcttcta tccgctgata aatagtgcca
     2041 ttcatcacag attctccaga ggtaatgtag ggattgtttt aattcccgct cccttatggg
     2101 agaaggttaa cgctcgggta acccttgccg aatgtaggcc ggataaggcg ttatacgccg
     2161 catccggcaa tcaatgcctg atgcgacgct gtcgcgtctt atcaggccta caaaccgtta
     2221 ccgactcgca tccggcaatt gtgggttacg atggcatcgc gatagcctgc ttctcttcaa
     2281 gcagcttctc gactacgcca ggatcggcaa gcgtcgaggt atcgcccagg ttgctggtat
     2341 cgcccgccgc aattttgcgc agaatacggc gcataatttt gccggagcgg gttttaggca
     2401 gggagtcggt ccagtgcagc acgtctggcg tcgccagcgg gccaatctct ttacgcaccc
     2461 agttgcggac ttctgcgtac agttctggtg acggttcctc cccgtgatta agcgtgacgt
     2521 aggcgtagat cgcctgacct ttaatattgt gcggaatacc gacgacagcg gcttcggcga
     2581 ttttcggatg cgccaccagc gccgactcaa tctccgccgt tcccagacgg tgaccggaga
     2641 cgttcagcac atcgtccaca cgcccggtga tccagtaata gctatcttca tcacgacgcg
     2701 cgccgtcgcc gctgaaatac atatttttga aggtggaaaa ataggtctgc tcaaaacgtt
     2761 cgtgatcgcc aaacagcgta cgcgcctgac ccggccagga gtcggtgatc accaggctac
     2821 cttcggtagc cccttccagc gggttacctt cgttatcgac cagcgccggt tgcacgccga
     2881 agaacggacg tgttgccgaa ccggctttca gctcggtagc gccaggcagc ggggtgatca
     2941 tgaaaccgcc ggtttcggtc tgccaccagg tatcgaccac cggacatttc tcgttgccga
     3001 tttttttcca gtaccactcc cacgcttccg ggttaattgg ctcgcccacg gaaccgagaa
     3061 tgcgcagcga cgaacggtcg gtgccttcga tcgctttatc gccttccgcc atcagcgcgc
     3121 gaatcgccgt gggcgcggta tagagaatat tgacctgatg cttgtccacc acctgtgcca
     3181 tacgggcagg cgtcggccag ttcggtacgc cttcaaacat cagcgtggtc gcgccgcagg
     3241 ccagcgggcc gtacagcaaa taactgtgtc cggtcaccca gcccacatcg gcggtgcacc
     3301 agtagatatc gcccggatga taatcaaaga catatttaaa ggtcagcgcc gcgtacacca
     3361 gataaccgcc ggtagtgtgc agtacgcctt ttggttttcc ggtagaaccg gaggtataga
     3421 gaataaacag cggatcttcg gcgttcatct cttccgcctg gtgctgatcg ctggcttgct
     3481 caacctggtc gtgccaccac aggtcgcgcc cttcctgcca gtcaattttc ccgccagtac
     3541 gcttcagtac caccacatgc tctacgctgg tgacgttcgg gttttttagt gcgtcatcaa
     3601 cgtttttctt cagcggaata ctacgcccgg cgcgcacgcc ttcgtcggaa gtgatcacca
     3661 gtcgtgagtt ggaatcgata atgcgcccgg caaccgcttc cggcgagaag ccaccgaaaa
     3721 ttaccgaatg caccgcgcca atacgggcgc aggccagcat cgcaaccgcg gcttccggca
     3781 ccatcggcat ataaatcgcc accacatcac cttttttaat gcccagcttg agcagggtat
     3841 tggcgaagcg gcagacgtcg cggtgcagct ctttatagct gatatgtttg ctctggctgg
     3901 cgtcgtcgcc ttcccagatg atggcggtac gatcgccgtt ttcttgcaga tggcggtcaa
     3961 ggcagtttgc cgccagattc agcgtgccgt cctcgtacca tttaatggac acattaccgg
     4021 gggcaaagga ggtgtttttc accttctggt acggtttgat ccagtcgaga atttttccct
     4081 gttcgcccca gaaggtatca ggtgcgttaa tagattgttg atacatcgcc tcgtactgct
     4141 gagggtttat caggcaacgg tctgcgatgt tggcaggaat ggtgtgtttg tgaatttgac
     4201 tcatgctttt gttctccttg taggatgtta ataatatgtg gcataagcgt taaatgtagg
     4261 ggtattggca gttttgttta gtatttgggc gacagatcac gcaaaagtag aattgtgcaa
     4321 ataaacggca gggtaatttt tgaaggtcag caacaaaagt tgattaattc tttgaggaac
     4381 atgcagttat gcatgctgtt gaaaaagagg aagatactga ctaactctaa agtggtattt
     4441 tacatgcact tacaattgat taaagacaac attcacagtg tggttatttg ttacacttag
     4501 gggcgagcaa tgtcatgaca gtgtaggtgc ggttactgtc gtgaaaaaca ataaaaaccg
     4561 ccattgcaac aatggcgcaa ttcggatgaa gcccctatga caaggataaa aataaacgca
     4621 cgccgtatct tcagcttatt gattcctttt ttctttttca cttctgttca cgctgaacaa
     4681 acggctgctc ccgcaaaacc tgtaactgtg gaagcgaaga atgaaacctt tgccccgcag
     4741 catcccgatc aatatctctc ctggaaagcc acctcggaac agtcagagcg tgttgacgcc
     4801 ctggcggaag atccacggct ggtgatcctg tgggcggggt atcccttctc gcacgattac
     4861 aacaagccgc gtggacatgc ctttgctgtg accgatgtgc gtgaaaccct gcgtaccggt
     4921 gcgccgaaaa acgctgaaga tggtccgcta ccgatggcgt gctggagttg taaaagcccg
     4981 gatgtggcgc gtctgatcca gaaagacggc gaagatggct acttccacgg taaatgggcg
     5041 cgcggcggcc cggaaatcgt caacaactta ggttgtgccg attgccataa caccgcctct
     5101 ccagagttcg ccaaaggcaa accggagtta accctttccc gtccgtatgc ggctcgcgcg
     5161 atggaagcca ttggtaaacc ttttgagaaa gccggacgtt tcgaccagca atcgatggtt
     5221 tgcggtcagt gccatgtgga gtattacttc gacggcaaaa acaaagcggt taaattcccg
     5281 tgggatgacg gcatgaaagt cgaaaatatg gagcagtatt acgacaaaat tgccttctct
     5341 gactggacta actccctgtc gaaaacgcca atgctgaaag cgcagcaccc ggaatatgaa
     5401 acctggacag cgggcattca cggtaaaaac aacgtgacct gtatcgactg ccatatgcca
     5461 aaagtgcaga acgccgaagg caaactctac accgaccata aaattggtaa tccgtttgat
     5521 aacttcgccc agacttgtgc gaactgccat acccaggaca aagctgcctt gcaaaaagtg
     5581 gtcgcggaac gtaagcagtc gattaacgac ctgaaaatca aggttgaaga tcaactggtt
     5641 cacgctcact tcgaagcgaa agcggcgctg gacgcaggtg cgacggaagc cgaaatgaag
     5701 ccgattcagg acgatatccg tcatgcccag tggcgttggg atctggcgat cgcttcccac
     5761 ggcattcata tgcacgcacc ggaagaaggt ctgaggatgc tcggtacggc gatggataaa
     5821 gcggcggatg cacgcaccaa actggcgcgc ctgctggcga ctaaaggcat cacccatgaa
     5881 atccagatcc cggatatctc gaccaaagag aaagcccagc aggccattgg cctgaacatg
     5941 gaacaaatca aggccgagaa gcaggacttc atcaaaacgg tgatcccgca gtgggaagag
     6001 caggcacgta aaaacggtct gttaagccaa taaccccgtt ccgcctcgca aggggcggaa
     6061 aacacaatgg agtgaatatg agcgtattac gttcgttatt aactgccggg gtgctggcgt
     6121 cgggcctgtt gtggagcctg aacggaatta ccgctacccc tgcggcgcag gcatctgatg
     6181 atcgctacga agttacccag cagcgtaacc cggatgccgc ctgtctggac tgtcataagc
     6241 cagataccga aggtatgcat ggcaaacatg cttccgttat caacccgaat aacaaactgc
     6301 cggtcacctg caccaactgt cacggccagc catcaccgca acaccgcgaa ggggtgaaag
     6361 atgtgatgcg ctttaacgag ccgatgtaca aggttggaga gcagaacagc gtctgtatgt
     6421 cctgccatct gccggaacag ttgcaaaaag cgttctggcc gcacgatgtc cacgtaacca
     6481 aagtggcgtg cgccagctgc cattccctgc atccacagca agatacgatg cagacgttaa
     6541 gtgacaaagg acggattaag atttgcgtcg attgccacag cgatcagcgc accaatccga
     6601 actttaaccc ggcgtctgtt ccgttgctta aggagcagcc atgacctggt ctcgtcgcca
     6661 gtttctcacc ggcgtcggcg tgctggcagc cgtcagcggc accgcagggc gcgtggtggc
     6721 gaaaacgttg aatatcaatg gggtgcgtta cggcatggtg catgacgagt cgttatgcat
     6781 cggctgtacc gcctgtatgg atgcttgtcg ggaagtgaac aaggtgccgg aaggcgtctc
     6841 gcgcctgacg attattcgta gcgagccgca gggcgaattt cctgatgtga aatatcgctt
     6901 cttccgtaag tcttgccagc actgcgatca tgcgccgtgc gttgacgtct gcccgaccgg
     6961 tgcatctttt cgcgatgctg ccagtgggat tgtcgatgtt aatccggatc tctgcgtcgg
     7021 ttgtcagtac tgcatcgccg cctgtccgta ccgcgtgcgc tttattcatc cggtcacgaa
     7081 gacagcggac aaatgcgatt tctgccgtaa gaccaatttg caggccggta agcttcccgc
     7141 gtgcgttgag gcttgcccga cgaaggcgct gacgtttggc aatctggacg atcccaacag
     7201 tgagatttcg caactgctgc gccagaagcc cacttaccgc tacaagctgg cgctgggaac
     7261 caaaccgaag ctgtaccgcg taccgtttaa atacggggag gtgagccaat gacgcagact
     7321 tccgcatttc attttgaatc gctggtgtgg gactggccga ttgccatcta cctgtttttg
     7381 attggtattt ctgccggtct ggtgacgctg gccgtgctgt tacgtcgctt ctacccgcag
     7441 gcgggcggtg cagacagcac gttgctgcgc accacgctga ttgtcggacc tggcgcggtg
     7501 atcctcggtc tgttgagcct cgtcttccac ctgacaagac cgtggacctt ctggaagctg
     7561 atgttccact acagttttac ttcggtgatg tcgatggggg tgatgctgtt tcagctttac
     7621 atggtggtgc tggtgctgtg gctggcgaaa atctttgaac atgatttgct tgccctgcaa
     7681 caacgctggt tgccgaagct ggggatcgtg caaaaggttc tgagcctgct gacgcccgtt
     7741 catcgcggac tggaaacatt gatgctggtg ctggcggtgt tgttgggggc ttataccggt
     7801 tttctgctgt cggcgctgaa atcgtatccg ttccttaata acccgatcct gccggtgctg
     7861 ttcctcttct ccggcatctc gtccggtgcg gcggtggcac tgatcgccat ggcgatacgc
     7921 caacgcagta acccgcattc cacggaagcg cagtttgtac accgtatgga aatccccgtg
     7981 gtatggggtg aaatcttcct gctggcggcg ttttttgtcg gtctggcgct gggcgatgac
     8041 ggtaaagtgc gtgcgctggt ggcggcattg ggcggcggtt tctggacgtg gtggttctgg
     8101 cttggtgtcg ccgggctggg gctgattgtg ccaatgttgt tcaaaccgtg ggtcaatcgc
     8161 agttccggca ttcctgccgt gctggcggcg tgtggggcca gtctggtcgg cgtgttgatg
     8221 ttgcgatttt tcattctcta cgccgggcag ttaacggtgg cgtaagccag aacagaggtg
     8281 gtttctggac gtattccttc ctgaagtcgg ttttctggcg ctgttgttaa gtctcggggt
     8341 caacgtgttg accccgttga cggcctttgc gggagtgcgg ttgcgctggc ctgccatgat
     8401 gcgactcact tgcatcggca ttctggcaca gttcgcgctc ctgctgctcg cctttggcgt
     8461 actgacgtat tgttttctca tcagcgattt ctcggtcatt tatgtcgccc aacatagcta
     8521 cagcttgctg tcgtggaaac tcaaactggc ggcggtgtgg ggcggtcatg aaggttcgct
     8581 gctgctttgg gtgctgctgc tttccgcctg gagcgcgctg tttgcctggc aatatcggca
     8641 gcaaaccgat ccgctatttc cgctgacgct agccgtttta tctctcatgc tcgccgcact
     8701 gctactgttt gtagtgctgt ggtccgatcc cttcgtgcgg atatttccac cagcaatcga
     8761 aggccgcgat ctcaatccga tgctgcaaca tcccggtctt atctttcatc caccgctgct
     8821 ttaccttggc tatggcggtt tgatggtagc ggcgagcgtg gcgctggcga gtctactgcg
     8881 cggcgagttt gatggtgcct gcgcccgaat ttgctggcgc tgggcgttac ctggctggag
     8941 tgcattaact gcggggatca tcctcggttc ctggtgggcc tactgcgaac tcggctgggg
     9001 cggctggtgg ttctgggatc cggtggaaaa cgcctcttta ttaccctggc tttctgccac
     9061 tgcgctgctg cacagtttat ccctgacacg ccagcggggg atttttcgcc actggtcgct
     9121 gttgttggcg atagttactc tgatgctgtc gctgctgggc accttaattg tccgttctgg
     9181 cattctggtt tcggttcatg cgttcgctct ggataacgtt cgcgctgtgc cgttgttcag
     9241 cctgtttgca ctgattagcc ttgcgtctct ggctctgtat ggctggcgag cgcgggacgg
     9301 tggcccggcg gtgcgttttt cggggttatc gcgggaaatg ttaatcctcg ctacgctgtt
     9361 gctgttttgc gcagtgctac tgatcgtgct ggtgggaacg ctttatccga tgatttacgg
     9421 cctgctgggc tggggacgtc tctccgttgg cgcaccgtat tttaaccgcg cgacgttacc
     9481 gtttggtctg ttgatgttgg tggtgattgt gctggcgacg tttgtctctg gcaaacgcgc
     9541 gcagcttccg gcgctgctgg cgcatgcggg catactgtta tttgccgcag ggatcgttgt
     9601 ctccagcgtc agccgtcagg agatcagcct gaatttacag ccgggtcagc aggtgacgct
     9661 ggcaggatac accttccgtt ttgagcgcct cgatctgcaa gccaaaggca attacaccag
     9721 cgaaaaagcg atagtggcgc tgtttgacca tcagcaacgc attggtgaat taacgccgga
     9781 gcggcgtttt tacgaagctc gtcgtcagca aatgatggaa ccgtcaattc gctggaacgg
     9841 catccatgac tggtatgcgg tcatggggga gaaaactgga gcggatcgtt acgcttttcg
     9901 cttgtatgta caaagcggtg tgcgctggat ctggggggga ggattgttga tgattgcggg
     9961 cgcattgtta agcggatggc gggggaagaa gcgcgatgta taaagggctt ctcacgctac
    10021 tgctgttatt tacctgtttt gcgcgcgctc aggttgtaga cacctggcaa ttcgccaatc
    10081 cgcaacaaca gcaacaggcg ttaaatattg ccagccagtt acgttgtccg cagtgccaga
    10141 atcaaaactt actggaatcc aacgcgccgg tggcagtcag tatgcgccat caggtttaca
    10201 gcatggtggc ggaggggaaa agtgaagtcg aaatcattgg ctggatgacc gaacgctacg
    10261 gagattttgt tcgctataac ccaccgttaa cgggtcagac gctggtgttg tgggcgctgc
    10321 cagtggtgtt gttactgctg atggcactga tcctctggcg agtgagggcg aagcgatgaa
    10381 acagcctcaa ataccggtga aaatgctgac aacactcacg atattgatgg tattcctctg
    10441 tatcggtagt tatctgttaa gcccaaaatg gcaggcggta cgtgcggagt atcagcgtca
    10501 gcgcgatccg ctacatcagt ttgccagcca gcaaaacccg gaagcgcagc ttcaggcatt
    10561 gcaggataaa atccgcgcta atccacaaaa cagcgaacag tgggcgttac tgggcgagta
    10621 ctatctgtgg caaaacgatt acagcaattc gctgctggcg taccgtcagg cgttgcaact
    10681 acgtggtgag aacgctgaac tgtatgcggc gctggcgacg gtgctttatt accaggccag
    10741 ccagcatatg accgcccaga ctcgcgcaat gatcgacaaa gccctcgcgc tggactctaa
    10801 cgaaatcacc gctctgatgc tgctggcttc cgatgcgttt atgcaggcga attacgcgca
    10861 agccatcgaa ctatggcaaa aagtgatgga tctcaactca ccccggatca accggacaca
    10921 gctggtcgag tcgattaata tggcgaaatt gttgcagcgg agatcggatt aatcatctgg
    10981 tgatgaagat tatgttattg agctgcataa aaaataatcg aatgaacata tgccaaaaat
    11041 aatcactaat cagtattatt gcagattaaa caaataaaaa tctttccata acaaatggtt
    11101 attcattaat cctgccataa actcgcgtta ttatgcatta atgcagcgaa aagctctgtt
    11161 gttaaagggt tgcgcaacat accgcgcaaa tgatactgat cataagcgtt aaaaaaatct
    11221 acaaaccaac gcaacacaat tcatgccctg gcagtatgtc acgttctcgc gtttctgaac
    11281 ggggaacggc gctccattga ggaagtcatt catatgaaaa atataaaatt cagcctggcc
    11341 tggcagattc tgtttgctat ggtgctgggc attctcctgg gaagctacct gcactaccat
    11401 agcgacagcc gcgactggct ggtcgtcaat ttgctctctc cggcgggtga tatcttcatc
    11461 catctgatca aaatgattgt tgtgccgatt gtgatctcca cgctggtggt gggtatcgcg
    11521 ggtgttggtg atgccaaaca gctcgggcgt attggcgcga aaaccattat ctacttcgag
    11581 gtgatcacca ccgttgccat cattttgggg atcactctgg cgaacgtctt ccagcccggt
    11641 gccggggtgg atatgtcgca gctggcgacc gtcgatatct cgaaatatca gagcactacg
    11701 gaagcggtac aaagcagttc ccacggcatt atgggcacga ttttgtcgct ggtgccgacg
    11761 aatattgtgg cgtcgatggc gaaaggcgaa atgttgccga tcatcttctt ctcggtgctg
    11821 tttggtctgg ggctttcctc cctgcccgcg acgcatcgtg aaccgctggt gaccgtgttc
    11881 cgctccatct ctgaaaccat gtttaaagtg actcacatgg tgatgcgtta tgcgccggtg
    11941 ggtgtgtttg cgctgattgc agtgacggtg gctaactttg gtttctcttc tctgtggcca
    12001 ctggcgaaac tggtgctgct ggtgcatttc gccattctgt tcttcgcgct ggtagtgctg
    12061 ggaattgtgg cgcgcctatg cgggttaagc gtctggatcc tgattcgtat tctgaaagat
    12121 gagctgattc tggcgtactc cactgccagc tctgaaagcg tactgccgcg aattattgag
    12181 aagatggaag cctacggagc accggcgtcg atcaccagct tcgtggtgcc gaccggttac
    12241 tcttttaacc ttgatggttc gacgctgtat caaagtattg ccgctatttt catcgctcag
    12301 ctgtatggca ttgacctgtc catctggcag gaaatcattc tggtgctgac gctgatggtg
    12361 acctcgaaag ggattgctgg cgtgccgggc gtgtcgtttg tggtgttgct ggcaacgctg
    12421 ggtagcgtag gtattccgct ggaaggtctg gcgtttattg ctggtgttga ccgtatcctc
    12481 gacatggcgc gtactgcact gaacgtggtg ggtaatgcgc tggcggtgct ggtgattgcc
    12541 aagtgggaac acaaatttga ccgcaaaaag gcgctggctt atgagcgtga agtgctgggc
    12601 aaatttgata aaactgcgga tcaataattg aagattgccg gggatatccc ccggcaatgt
    12661 gtggatgcct gatgcgacgc tggtgcgtct tatcaggcct acgtcagaca gcgctagcgt
    12721 gattttgtag gtctgataag gcgtttacgc tgcatccgac atcaacgcct gatgcgacgc
    12781 ttaacgcgtc ttatcaggcc taccttaacc gttagttaat ttttcaaact cttcacaccc
    12841 ggtatcaaac ccttccatac agctcaggtt caaccagtgc agcgcctttt gcttgttctt
    12901 ctcgataaaa ccttcttcac cgtttaagaa catcattccc gcccagtact cggaataacc
    12961 ggttcgggaa attgcagagc tgcgtttgaa ataccaggtt gccttgtcat cgtcggcctt
    13021 aatgcccacg ccgttggcgt aaatcaaacc aagcagcatt tgggcatcga cggcagagtc
    13081 gttctccaga tcttccgagg cgttttctaa cagcgaaatc gcctttggat aatccggttt
    13141 acccgcttga gtatttacca gaatatgcgc cagcgttact tcaccttctt tactgcccgc
    13201 ttgcgccgct ttctctgcaa gaacttttgc ttgtggatag tccagactga ccggattggt
    13261 gattttgatc tgcgccaaca gcgcgcaggc atcagcatca ccgctgtcgg cggctttctg
    13321 tgcccaatat tcggctttgc tcaaatcgcc ggagctaaac cagctgtcgg caagaaaata
    13381 ttgtgcgcgt cggtccccgg cctctgctgc ctttaaatac tggctgccag gctcggagtc
    13441 gttggcgtgg gcaaagaatg tcaaaaacaa catcaatgcg ataatttttt tcattttgat
    13501 aatacgattg ggtagggagt aaccagtata aagagttcga atggataaaa aaacagcctc
    13561 cgaaaggagg ctgtagaaag gactgtatta cgccagtgcc gcttcgcgca ggcgagtttt
    13621 caacttgttg tactcgtcaa tcacgtactg ctcggcggcg cgctgatcgg cgatcggctc
    13681 gacgcgcacg gcgcagtatt tgtattccgg cgttttcgta atcgggctta agttttcggt
    13741 aaccagctcg ttacaggcac caatccacca ctggtaggtc atgtaaatcg cccctttgtt
    13801 cggacgatcg ctgacctgcg cacgggtgat aattttgcct ttacgcgagt gcacccaaac
    13861 caatgcctca tcttcaatac tcagacgttt ggcgtcttcg gtattgattt gtgcgtagcc
    13921 aggttcatca gccagcgccg ccagtgccgc acagttaccg gtcatcgaac ggcaagagta
    13981 gtgaccaact tcacgcaccg ttgacagtac catcgggtac tcgtcggtga gtttgtcgat
    14041 tggcgctacc cagtcgcagg tgaagaactg cgccagaccg ttcggggtgt caaacttctc
    14101 tttaaacaga taagaagtcc cctgatcggc atctgaagta tcgcggcaag gccactgaat
    14161 gaagcccagt tcgcccattt tctcgtaagt cgcaccgtag aaatccgggc acagatgacg
    14221 caactcatcc cagatctcct gggtgttgtt gtagtgcatc ggataaccca tacgggtggc
    14281 gatttcactg atgatttgcc agtccgtttt cagatcccat ttcggttcaa ctgctttgaa
    14341 gaaacgctgg aagccacggt cagccgcagt aaacacgcct tcatgctcgc cccacgacgt
    14401 tgacggtaaa ataacatccg ccgccgacgc ggttttggtc ataaagatgt cctgaacgat
    14461 aaccagttcc agatcttcaa aggctttacg tactgccgac agctccgcgt cagtttgtag
    14521 cggatcttcg cccataatgt acgcggcacg cacttcgcca tgcgctgcgc ggtgcggcag
    14581 ctcgctgatg cgataaccgg tatgagcagg caggctttcc acgccccagg ctttggcgaa
    14641 tttctcgcgg ttagccggat cttttacgta ctggtatccc ggatacgtat ccggcagcgc
    14701 gcccatatcg caggcaccct gcacgttgtt ctgaccacga accgggttaa cacccgcatg
    14761 tggcttaccg aggttaccgg tcagcatcgc gaggctggtc agagaacgca cggtttccac
    14821 gccctggtag aactgggtta cacccatgcc ccacaggatg gcggcgcttt tcgcctgggc
    14881 atacatccgt gccgcctgac gaatctcact ggcgctgacg ccggtgatat cttcaaccga
    14941 ctccggcgtg tagccttcaa cgattttacg atactcttca aagccttctg tacgggaagc
    15001 gacgaatgct ttgtcgtaca gattttcttc aataatgaca tggcccatcg cattcaacag
    15061 cgcgatgttc gagccgtttt tcagtgcaat gtgcatgtca gcaatgcgcg cggtttcaat
    15121 tttgcgcgga tcgcagacga taattttcgc cccgttacgt ttagcgttaa ttacgtgatt
    15181 cgccacgatt gggtgggaat ccgccgggtt gtacccgaaa acgaacacta aatcggtatt
    15241 atcaatttcg ttaatagcat tgctcattgc gccattaccg accgattggt gcagacctgc
    15301 aaccgatggg ccgtgtcaga cacgagcgca gcagtcaacg ttattggtac caataacggc
    15361 gcgcgcaaat ttttgcatta catagttggt ttcgttaccc gtaccacgcg aggagccggt
    15421 cgtctggatg gcatccggac cgtacttctc tttgatggcg ctcaggcgct cggcaacgta
    15481 attcagtgcc tcatcccagg aaacaggttc gagtttgccg ccacgctggc gacggatcat
    15541 gggggttttc aggcgcgggg tcaggatctg ggtatcgtta atgaagtccc agccataata
    15601 acccttcaga cacagggtac cctggttggt tttcccctgc gctgcctccg cccggacgat
    15661 tttgccgtta tcgacgacca ggttgatttt gcaacctgat gcgcaatagg ggcaaaccgt
    15721 gacgactttt ttcatcggtc tcgctccagt taatcaaatc acgcatacgc gctctcgact
    15781 acagtatgca tcttttatgc cacattttat ttggggtcat tccctgatat tacgggcact
    15841 atttattcaa aactctgacg aaaaacaggc tgtcgtcagt tttgacgtga cgaaacgaaa
    15901 taccgcgtga cagccatcac acggcagaca ttttattttt tctcgacgac gggagccgcc
    15961 tgatacccgc tgcccagcga tttcatcaac tgaatgcttt ggatcacccg gcggctgtcc
    16021 agcatcagta atgacatctc ttcggcaagc accggcaacc gggcttcggt agcctgtaag
    16081 cggctggtta agccgcgctg ataggcggcc tcggcagcgc gctgggtgaa gcgcgttgct
    16141 tccacgcgtt cagcctgcat ttctcgctcg tcgttgagcg tttgcagacg cgtgccgttg
    16201 acggcaacgt cacgcaccgc gttcagtact gactggttgt aacgttcaat catcatgttg
    16261 ctggcggcgc gagtgccttc gagattggca ttcaaccgtc caccgtcaaa cagcggcaat
    16321 ttcagacccg ggatgaagtt gaactggcga ctggtttttt tgaataaggt atccaggtgg
    16381 atggagtcca gaccgaaaaa cgctttgata tcaaagcttg ggtagaacag cgcccgcgcg
    16441 gaatccacct gatctaatga cgcctgaaca taccagcgca tggcttgcag atccgggcgt
    16501 ctggcgagca actcataaga gagtgtcgcc ggaataccgg tctggactcg tggtaatgcc
    16561 actggtttga tctccggcat atcgctggct cccgcgccaa tcaatgcacg cagagattct
    16621 cgcgtttcgg tgatttgccc tttaacggca gcaatttgtt tatcgaccgc cagaatctgt
    16681 gcccgcgcgc cgtggaaagg cacttgcgct tccagaccgt gcgccacttt actttggtgc
    16741 gctttcaccg cgtaatcaat cacatcgcga gtttgttcta acagatcgag catctgatag
    16801 ctggcctgca tactgtaata aagctgcgct acgcccgtgg tcagcgatag ctctactgct
    16861 gcggtttctg ccagcgcggc attatgcgcg ccaatggcgg cggtaaccgc tgagcgatgc
    16921 acaccccaca aatcaagatc cagtccggca aacaaaccga ctgtggcttc cgtatagtac
    16981 ggcccgtcca tacccagtgc tggcgcatcc atcgcataag ggcttaaaaa gccgttcgcc
    17041 gagacgcgtt ggcggttgag catccctaac gctgcgacct gtaattgtga accggcatct
    17101 aacaaatcgg cctgggactg cgctttttct tcccgcagtt tcgcttcggc gagggtgtgt
    17161 gaaccactta gcgtccgttg gatcagcgca tccagctgcg ggtcattgag ttgtttccac
    17221 cactgcgcct gcggccagcc ggagctggca agatgaatat catcggccag tttgatttgt
    17281 tccggtttga gctgttgatg aggcgcagaa tccttgcgta ccagggcaca gccggaaatc
    17341 agcgtcgtgc tgccgagaat gctgcacaac agcagacgtg aaagttgacg attgatcatt
    17401 gcgtggctcc ttgtgcctgt tccgtggcgg gcgttaatgc cggggctgtc cagtctggca
    17461 agcgggcgaa aagctggcag acgtgttgct cctgttttaa taaggtgggc aggatggcct
    17521 gcgaggcggg tgtctcttca agcgttattt gaggagaacg gctgagtgcg gttgccagac
    17581 cggcagcgta tttttccagg gcgtcggcaa actgacctgc tttgtccggc tgtagtgcgt
    17641 tatccagcgc ctgcgccgag ttccaggtgg catcccaggc gtgaagaaga tcgcggccct
    17701 gatgaataac cgtttgcgaa cgttcaatca gtaaggcgcg ttcttcgctg tccagttgac
    17761 gctccagcgc cacgcgttgg cacatctctt cacaggcatt aaacgccgca tgcagaccta
    17821 tacgaatttg cagataagtg cgcagagccg tgacttcctg ctggcgtggg atccgcatta
    17881 ctttacttag catacccagc gcgccagcca gtttttgcgg cagcgtgcgc gcttcacttt
    17941 caggccagac aaaggtgtaa atcaccgcag acaccacggt accaatgatg atacccaggg
    18001 cgcgatcgcg aatttccacc aggtcgtaca ccggaccaaa aacgttttcg agcgtggcga
    18061 gcgcgaaggt gaccaccatc tgtgtgccga tataagaaga gcgttcagag ctggtggcaa
    18121 tccatgcgcc caacaggaaa atcggtgcca gcacaaacag caattcgaca atattgtcca
    18181 gccagggcat gaccagtagc gtgaataaca gcgccagaat cgcgccgcaa aaggccccgc
    18241 caaaacgcag caccatcttc tggtacgatg aaccgacatt cggattagcg acgatcacgc
    18301 aggtcagcat acaggtgtga atgccttccc agtccacgcc gctgtagaag gtgtaacaga
    18361 tcaaacaggc gagcagcgtt tttaccgcgt agcgcatata gtctggattg gtaaaagcat
    18421 cagcgaccat tgatggcggt ttggcggcgg gcgttggcgg cgtattcggg tccatctgac
    18481 ccagttgtaa caacgtctgg cagatattct ccaggttaca ttcccgtgcc gccatcgctt
    18541 cactttcact gatccgccag tcgctttgcc agcactgacc ttcggtaatg gcatgctgca
    18601 gcttgttgat ttctgaagct aatttttgtc ggaattcaat aattgcctga gaatcagcaa
    18661 aagaggtggg atcgtagcga ttcagcgtcg agtaaatgta ggttaccgtt gccacgcagc
    18721 tttgccacca tgcgctttga gttcgccagt tggcatcgtc cgcgaggcaa aagacattga
    18781 gtttttgcag cgccagcgcc tctctttcaa tccgcgtttc gggtagcggt gcgaggctgt
    18841 ccgtcaggtg gctaatggca tcatcaagcc gatcattaag cgcttgatgc atttgcgaaa
    18901 tggcacgact gggaaaccac agtacgccga ttaacgtcat taataacgtc gggtagaggc
    18961 caacaacgat acaccacagc gttaagcgca cgaccacttc cggatagtcg agcatggcgg
    19021 ggaaggtttg cccataaatg gcgacaatgg cgacagcgaa aaagaccagt cccaaccgat
    19081 gggtgcgcat caaaaacatg cagcccatca ggatcggccc ggcgatgatc aagcggatca
    19141 acggttcgcc gtatgaccat ttgtagatca aaaacaggct gccgatctcc agcaccgtgg
    19201 caaccacaaa caagatcgcg acaaatttgg tgtaaaacgc gttcgactga ataccgtaaa
    19261 acagcactgc cagcgataac gccacaaaag ggatctcaaa agtcatcgag atcagaatca
    19321 ccagcaggca gcctacccag agttgcacgg tctgcggcac gcgacctggt cgccgctcgc
    19381 ttaactcttc atgaaagaac gccagcagcc tgaccaccgg taatggcagg gagttgagcg
    19441 cgctcattat tgcggctcaa gattagcgac tgccgaagcg ccgatgcgga acatttccgg
    19501 gtcaggttta tcaaccatga ttttgaccgg aaaacgctgg gcaacacgga cccagttaat
    19561 agaacgagaa actttcggca ggccgcccag caccaggccg ccgtcatccg gtagcacgcc
    19621 gtagccaatt gaatccactt taccctcgaa agttttgccg ctgtcactca tcaggcgaat
    19681 cgttgcaggt gtacctgagc gaatattttt cagatcggtt tcgcggaagt tggcgatcac
    19741 gtaccagtga cgagtgtcga ttagggtaaa aatagggcgc atggcagaag caaattgccc
    19801 gacggaggtt ttgagggaaa tgacccggcc atcaaacggt gcgcgaacgg tcgccatttc
    19861 cagatgcagt ttggtcaggg caatatccgc ttcgaccgcc gcacgctggg caactaatgc
    19921 atccacgccg ctgacggcgc tggcggctga ctgcgcctgc aacaatacag cattaagatc
    19981 ggcttctgca gcgcgctgcg cggtcctcgc acggtcaacg tcttccgctg agacaaaacc
    20041 ttctttcagt aatggctcgg tgcggcgtaa tgtatctgtg gcctgtttag cggcggcacg
    20101 ggctttttct accgtggcgt taaccgagtc ggcaccaaac tgttgcgcgt caacgctacg
    20161 ctgggtgagc ataatttgct tatccagtgc cgcgagggag gcttcagatt tcgccagatt
    20221 ggcttcgtac gggcgcgggt caatgcggaa cagcaaatcg ccctgtttga ctgcctggtt
    20281 gtcggtgacc gccagttcta caatgcggcc gctgacttcc ggcaccacat caatggtatc
    20341 tgctgacacg taagcgtcat tagttgatgg cgcactgtct acgcgccaga taacgaaaac
    20401 aagggcaacc agcgccaacg caaccactaa cagagcaggg aatttactgc gaggagcttt
    20461 tttcggcgta ctttccatga gcgcgtctca aatagattag aaaaatgcca gccagaacag
    20521 catggcgtag agagcaaaaa gggcggtata aataattccg acaaatgcca gcttgatatt
    20581 tgcccgctga ataactcgcc tcgtgatgag cgtcaatata aggctggcaa tggcgcagaa
    20641 aaaccagctg ggataataag cgccgatcac cgggattgcc ggagataatg agcatcccga
    20701 aaccatgaag acgggaatta tccaggctgt ttttttgagt tgcatcgcca tgcgagagag
    20761 aactgttggc atattaaata catcctgtct gctgcgaaca atatctgtag tgaatatcaa
    20821 tatccattga atgcgtcagt atggctattt tcttgatgaa taaaaatagc tttattcagc
    20881 caccgcattc ataacttata tattttattt attaattggc tgtatatatt tttaaaataa
    20941 atgcttttgg gggcttcagc gcccccaaaa gcgagtgaca ttaatttggg gttacgatat
    21001 tcacccacaa atcgaaatta tccagacagg cgataatttc ttccagtttc gcgccattgc
    21061 caataatttt ggctttcttc gctttcacca gatccgccat ttttgcctgt ccggtcagca
    21121 cggcgtgcag atcttcacgg ctgatgtaga aagaggcgtc ggcttgcggt tgcagtgttt
    21181 tacggtagtt aagcacgcta tcgttcagcg tcaggttgag gttatcgccg ttgctcatat
    21241 tgaagttcag gctgatattt ttacccgcag ctttcgcgct atcgaggcga acggccataa
    21301 agtcgaacag catttcgacc gacattccgc gaatggtgtc cggggaaccg gtggtgccgt
    21361 ggctgaactt atgcacccct tcgcgcagct ctttcgcgcc ggtcaggtag aaaccgcgcc
    21421 atgtggcgga ctcggcctga tagcccagct gttcaaagtt attcgcttgc agattcttcg
    21481 ccacctggtc acccgggttg gcggcaatca cctgtttcag tagttctgcc gaccagcggt
    21541 aatcaccttg cttattcgct tcttgcgcca ggttgatgac acgggcagag ccgcccagcg
    21601 ctttcacgta acgtttgccc atctctacct gaccatacgg atgcaggtta gccgggttac
    21661 cgtcgtaata gccaagatag aagttataca ccgcgcgggc gttgtggctg acagaaccgt
    21721 aatagccacg gctggcccag ttattggcaa gtgcaggcgg cagcttaatc atgtcgccga
    21781 tttcattcat ggtgtagccc tggttcgcca ggtgcagggt ctggtcgtga atgaacgtga
    21841 tagtatcgcg atatttacca atataatcat tgatatgctt attgccccag accggccatg
    21901 tgtgcggcat aaacagtact tccgcgtcgt taccccacat atccagcgtt tcgttcaggt
    21961 actcggtcca cttgctggta tcgcgggttt tcgcgccgcg cagagtgtag aagttgtgca
    22021 gggtatgcgt ggcgttctcg gcggtacaca gggctttcag ggccggaata tagaagtgca
    22081 tttcggctgg cgcttcgcta cctggggtca tcaggaagtc aaactccagg ccgtcgataa
    22141 tcatcttctc gccagttctg acgatagttt tagtcggtgc aataatgctc gggtcgcccg
    22201 ttgccagcgt cacgccaagg ccattaccca cgttgccttg cgcgttgtgc ggcagtaaca
    22261 gaccgtaaga gtagagcgca cggcggctca tgatgttacc cgccagcacg ttttcgctga
    22321 tggcttcgtc cataaagcca gcaggggcaa tcacctgaac tttgccggat ttaacatcgg
    22381 cttcagagat aatgcctttc acgccaccat agtggtcggt gtggctgtga gtgtagataa
    22441 cggcaacaat cggtttttgc ggacgatgct ggaagtaaag gtcaagtgcg gcttttgcgg
    22501 caggcggcgt caccagcggg tcgatgacaa taatgccttt ctccccctca acgaacgtaa
    22561 tgttagagat atcctggccg cgcacctgat acattttatc ggtgactttg aacaggccag
    22621 aaatgccgtt gatctgcgac tgacgccaca ggctggggtt tacggtttcc ggcgcagcgg
    22681 cattaatatc aaatttgtaa tcgtctgcgc ggtagtaaac tttaccgttc gcatcacgca
    22741 gaataccttc atccagcagc ggggcgataa atccacgctg ggcatcgtca aaatcctgac
    22801 gatcgctaaa cggtaatgat ttggcgtaat tctgattaac ctgttgggta tattgagtgg
    22861 cggctttcgc ttcctctttc gcgttagcgg tatttaccat catgccgctg gcggcagtta
    22921 acgcaataac aatcctgctc aaacggaata accgagagtt attcatataa atctccattt
    22981 atcatttatg tcgtaaatat gtaatccatg caaaatacgt catttaaagc tggcgttatc
    23041 ataattggga aattgcgact ttaataagtg gaagtgtgag cggaacgatc cattttatta
    23101 ggcatttgtg ctgaagaggg aaaagtacga catatttatt actatcatca ataatgtgac
    23161 ctgacttata tttttattat ttatattact ttcagctttc acttcgacaa gaagtacgca
    23221 caatgattaa tcagatgaat gatgcggagt gtaaccatga aacccgtcgt gctggtggtg
    23281 gatgatgaca cggctatctg cgcactgttg caggacgtgt taagtgaaca tgtctttacg
    23341 gtttcagtct gccataccgg acaggaggcc atattgcgta ttgagggcga tcctgacatt
    23401 gcgctggtgg tgctggatat gatgctaccc gataccaacg gcctgagggt attgcagcaa
    23461 atccagaaac tgcgtccgac attgccggtg gtgatgttga ccggcatggg cagtgagtct
    23521 gatgtggtgg tcgggctgga aatgggcgcg gatgattaca tctgcaaacc gttcaccccg
    23581 cgggtggtgg tcgcacgact gaaagcggtg ttgcgacggg ttggcgcgct ggcggttaat
    23641 gacgagaaaa gcgccgggct gtcattcaat ggctggtatc tcgataccat gcgctgccag
    23701 ttgcacaatc cgcttcagca acatgtcgag ttaactcagg gcgaatatgg cttgttactg
    23761 gctctggcgc aaaacgcgcg tcgggtattg agccgcgagc agttgctggc gttcacccac
    23821 agcgacagcg tggaggtgtt tgatcgcacc atcgacgtat taatcatgcg cttacgacgc
    23881 aaaatcgaac tcaacccgca tcagccgatg ttgattaaaa ctctgcgcgg tctggggtat
    23941 gtcttcgctg ccgacgtgca ttaatccgac atttctgttt gcagctcttc cagcgttttc
    24001 gccccgtcga tggcattggc gcataacagg cttgcccgcg cgcaggcgtg tgccagtttg
    24061 atgctctcgg taagcgtcca gcgttcatgg cagccatata aaaatccggc acaaaatgca
    24121 tcgcccgcgc cgacgctgcc gataatttcc cgttgttcta acttttgcga agggatccac
    24181 acgccgccct gttctggggt ctctccccac gccccttccg ggtagtggat caccacccgc
    24241 tggcgcacgc ccgcatctaa cagttcacgc gcggcggcag caatatgcgt gatatgcggt
    24301 tcgccgttgg gtaaccgaat ttccagcccg ctaaactccc cggcctccag ttcgttaatt
    24361 accagataat ccagatggcg caacgcgggc agcaccagcg gctgataacg cggatcgccc
    24421 ttacgggaga ccaggtcgag tgaggtttca aagccagtat cacgcatttg cgccagcagg
    24481 cgtgcgctac gggtgccgta aacatcgtcc ggcatatcca ggctgtcgag caacagtaaa
    24541 tagccgagat ggaaaatctt catggtgtta tcgagctgat cgaacgccgg aagatccaaa
    24601 agtctgtttg cacccggcga atggaaaaac gtccgctgcc cgcttgggtc tgtcatcacc
    24661 tgcgacatcg acgtcggggc aaaggtagtg cgctgcacat gctgacgatt gacgtgatat
    24721 tgctcaagca tcgccatgat gtaatcgccg tcgctgtcct gaccgattag cccaaccgcc
    24781 tgtaacggca gcccggtgcg cattttcgcc agcgtcagca ggacgttaag cggcgcaccg
    24841 ccggtggagc gttcgctgtg ggtgatctcc gccagccagc cgcgctctgg ccactggata
    24901 atctgatgga cgtggtcaac caacatattg cctgcggcga taatcccttt acgctccatc
    24961 atgcctgccc cgcactgcca aaaatacgca tctgttgtgc aaccgtatcg ctgatggcgg
    25021 cttccactgc cagcagtaac tcggcaaact cgtcatacat cggctgacgg tgcgccattc
    25081 gggcctcaac ggcagccagc gccgcttgcg acatgccagt gtagaaattg attttgtgga
    25141 tacccagttc gatggcgcgg cggaaatcgg catcgctaat ccctgagccg ccgtgtaata
    25201 ccagcgggat agcagcctgc tggcggatag cgtcgaggcg cgggaaatcg agtttcggtt
    25261 cgccgcgata tttgccgtgt gcattgccaa tcgccacggc cagcgcgtca atgccggtac
    25321 ggtcgacaaa atcacgcgcc agttgcggat cggtaaacag ggattcatcg gcatgaccat
    25381 agagtgcacc gccttcgtcg ccacccaccg cgcccagttc agcttctacc gatacgccaa
    25441 cggcatggca cattttcacc acttcccgcg tctggcggac attttcttca taccccagcg
    25501 tggagccatc aaacattacc gagctgaatc ccagccgcaa cgcttgcacc accgattcaa
    25561 aatgcagtcc gtgatcgaga tttaacacca ccggaatgtc atgacgcgcc gcttcaaatt
    25621 tcactgtctc gaccagggaa tccagcgaga tatatttaaa atgtacttcg gcaatgttga
    25681 taataaaggg cgaacgttct tgtctggcag cagcaaacaa ggcgcgtaaa aagtgcgagt
    25741 ccagcacatt aaacgcacct aacgaatagt gatgttcgcg ggcatgtgcc agcccttcag
    25801 cgagagaaat caatggcata acgttatcct tattcagcgg aactggcggt attcgttaca
    25861 caataccaac tgtgcaggtt cgtcttcgtc aatcaggttg tagcggtcca gtggctgtaa
    25921 aaagtggtta tcgtggtcgt cgtcattaac agaagagact tcgccaacca gcacatcgcc
    25981 aaaaccggct tctgcccaaa agctgtgata caggccgggc ggcaggcaga tactttcgcc
    26041 gggagacagg cgcaactgag agcctgcagt atgtttctgg cggcagccgt ctatcaccac
    26101 cgtaatgtca ctatcagccg tttgctcgtt actatcggca ttccacagtt caacaattaa
    26161 attaccgccg ccgcgattga taatgtcctc acgcttacgc cagtgaaaat gcattggcgt
    26221 gacctgcgca tcgcgcacat gcatgatctt ctcggcgtaa catttcacat acggcattcc
    26281 ttttgctgag ccgttacgta gggtaaacag cgtcagcccg tgtgctgcaa agttgttgcg
    26341 accaaaagcg gtaacatccc agccgagttt aagatcaaaa acctcctccc acgcggcggt
    26401 gtcgagctgc tgccattgag caggcgagaa actggcaaag ggcggcagat gtacgtcatg
    26461 ttgcgaaaag aattgccgtg tatggccgag aatgtcgttt atagcggagc gtttcatcga
    26521 aagcgttctc cttagcggtg accgggcagc tgcgccgccc ggcgtgtgga cttatttcac
    26581 cgtccagcct ttgtaattgc cgacgttgtt tttatcgatc atcgtcaccg ggatcagcac
    26641 cggctctttc ggcgcaggtt tgccctgcaa aatgtcgtag ccaatttcca ccgctttcgc
    26701 tgccatcacc tggggatctt gcgccggagt agcgacaaac aacgagttct cacgcttgag
    26761 cgcctcttcg gcgtccgggc ttccgtccac accgacaata aagaactcgt tacgttgtgc
    26821 ctgttttgct gccagatcgg caccgattgc ggtgggatcg ttaatcgcaa atacgccgtc
    26881 gatcttcgga tttgccgcca gcagtcccgt cattacctcc agcccgcctt cacgactgcc
    26941 tttcgcgttc tggttgtcag agagcacttt gatgtcagga tggcgtttaa attcagtctg
    27001 gcatccttcg acgcggtttt gcacggcgga taccggcggg ccgttgatga tcaccacgtt
    27061 gcctttgcct ttcaggcgat cggtgatgta tttacaggcc atttcgcctg cctgggtgtt
    27121 atcggaagtg atggtggcat cggcaccttc tgccgctacg tcaaccgcga cgaccacgat
    27181 cccggcatct ttcgcccgtt tcaccgccgg gccgatccct tttgaatcgg cagcgttgag
    27241 gataatcata tcgacgttag cggcgataaa gttgtctatc tgcgacacct gctgacccag
    27301 atcgtagccg ctggaaacca gcgtcacttt gacgttatcg cctgccagtt tgcgtgcttc
    27361 cagctccgcg cctttggtga tctgcacgaa gaacgggttc gccagatcgc ccaccgttac
    27421 gcctattgat ttcagttctt tcgcctgggc gaacggtgct gcggccagca gcgcgcctgc
    27481 acagagcgcg gtcactaagg gcttcaatct catgctgtat tcctcatgtg tttatgcact
    27541 ttgatggtgt cgggtacggt atttgtcgat cagcaccgca atgatgatca tcgccccttt
    27601 gatcaccagt tgccagaagt aagagacgcc cattaaggtc atgccattgt tgagcgtggc
    27661 gataattagc gcgccgacca gcgtgccggt gatggtgccg atgccgccga caaaactggt
    27721 gccgccgagg atcaccgctg caatggcatc cagttcgtaa cccatgccta agttgccgtt
    27781 ggcgctgtac agccgtgagg cgctcataat cccaccaagc ccagaaagca ggccgctcat
    27841 gccataaaca aacagcagca ccagccagac cttaatgccg gtaagacgcg ccgcctgcat
    27901 attgctgccg acggcgtaga tatgaacgcc aagcgtggtg cggcgcagaa taaaccagca
    27961 caccacgatc accgccagcg caatcaccac cagccatggg accggcccga gatagttgtt
    28021 gcctatccac tcgaaattaa tgttggagtt gataaccgtg gtgccgtccg ccagcagata
    28081 cgccgccccg cgcagcgccg tataggtgcc aagcgtaacg ataaacggtg gcaatccggc
    28141 aaaggccacc agcgcgccgt tgaacagccc cagcagcagg ccgagcatca gcgctgccgg
    28201 gatcgacagc atggcgaact cagggctaag ggagacgacc atcgccgcca ccgctgtggt
    28261 gccgaggatc gaacccacgg aaagatcgat cccgccggtc aaaatgatga aggtcatccc
    28321 cgccgccaga acaatgttga tcgatgcctg gcgggtaata ttcagcaggt tgctttcggt
    28381 aaaaaagttt ggcgtgataa agccaaatac cgccacgatc agaatcaaaa tgggcaagat
    28441 gccgaccgtt tgcatcagat cgctcatcag catttttttg gcagaggttt ttgcagaggt
    28501 cgtcatgagg ttaccgcctg ggaatgggcg tcgttgacgc cagtcgctaa ggtcataatg
    28561 ttttcctggg tgatgttttt gccgtttaac tcaccggcaa tgctgccttc gcgcatcaca
    28621 tagacgcgat cgctcattcc cactatctcc ggcagttcgc tggagatcat caggatcgcc
    28681 acgcccttgc gcgccatctc gttcatgatc cggtagatct cacttttggc gccaacgtcc
    28741 acgccgcggg cgggttcatc gagcagtaaa atgcgcgggc caatcgccac ccagcgggag
    28801 atcaacagtt tttgctgatt gccgccggaa agcccgcccg cgcgtacctg ggcatgaggc
    28861 acgcgaatgt tgagcaactt aatggcgtca tcggaaatgg tttgcgcttt tttgcgattg
    28921 agcattcccc agcgggcatc gcgctccagg gttgccatgg tgatgttttc ggctgctgcc
    28981 atttcaagga ataagccttg ttctttgcgg ttttcggtga gaaaaccaat gccaagatcg
    29041 atggcttcgc gcggggagtg gatcaccacc ggttcaccat cgacttcaat cattccgcct
    29101 gtcgctttcc gcacgccgaa gatcaactgc gccagttcgg aacgtccggc acccaccagt
    29161 ccggcgaggc caacaatttc gccggaacgc accagcaggc tactcggctg gactttgccg
    29221 ccgtcggtca ggtggtgaac attcaggcgg gctttaccga gcgggatatc gcgctcttta
    29281 ttgaacagat cgctcagtgg cctgccgacc atcatccgca ccagctcccc ggcattgagg
    29341 ttatcgcggg tcaggctgcc aacgtattgc ccgtcgcgta gcacgctgac gcgatcggaa
    29401 agctcataca cttccgccat gcggtggcta atgtagataa tcgccatccc ttcatcgcgc
    29461 agccgcataa tcagttcaaa caggcggtga gtttcgcggg aggagagggc agcggtgggt
    29521 tcgtccatca ccagaatgcg gctgttgcga tgtagtgcgc gggcgatttc gacctgctgt
    29581 tgctcggcaa tggtcagcgt catgacctta tcgctggcgt taaactgcgc gccgaggcgg
    29641 tcgatcactt tttgcgcctg cactagcatc tctttacgct gcaccagccc gccgtgggaa
    29701 agctcgctgc cgagaaaaat attttctgcc accgacagat tcggtgccag ctgcatctcc
    29761 tgataaatca gcgtaatacc ggcgcttaag gcatcttttg gcgtgcgaat gtgaaagggc
    29821 ttgccgtcta tgagaatctc gccgctggtg gcggtatacg cccccgccag caccttcatc
    29881 agcgtgcttt ttcccgcgcc gttttccccc atcagggcat ggatctcgcc agggtagacc
    29941 gtcaaatcca cccctttgag tgcataaaat ttgccgaagg ctttggcaat attgcgcatc
    30001 tccagaactg gcgtgctgtt catggtggac tcctgcgagg ttgtcgatat cagcactcca
    30061 tcacaggaaa gtaaatgcaa aatgtcagaa gccgttcata tttgtcatat tgcgattttt
    30121 cttcacggag cgtgatatgg cgcaccccgg acgtcacttt tttgccagcg cgcgcgggcg
    30181 attgctgctt ttgaatttgc tggtggtggc ggtgacgttg atggtcagcg gcgtggcggt
    30241 aatgggcttt cgtcatgcca gccagatgca ggagctggtg cagcagcaaa cggtggatga
    30301 tatgaccggc agcctgaatc tggcgcgcga cacggcaaac gtggcaacgg cggcggtgcg
    30361 attgtcgcag gtggtgggag cgctggaata taaaggcgaa gccgagcggt tgcaggagac
    30421 acagcgggca ttaaaaagct cgcttgcaca actggcgaac gcgcctttag cgcagcagga
    30481 agccgggctg gtgacgcgaa tcatcacgcg tagcaacgaa ctgcaaacca gcgtcggcgg
    30541 tatgctggag cgcgggcaaa ggcggcatct ggagcgcaac gcgctgttaa gttcgctgta
    30601 tcaaaactta agttatctgc ggcatctgca aaaagtcact cacgcgcaag atgatatttt
    30661 gcttaacgag atgaaccgac tgattgttgc cgctattgcc acccccgcgc cgcaggcgat
    30721 tattcatcaa ctggtcgggg taatgtctgc gttgccgacc cacagcgata cgccattggt
    30781 taatacgtta ctcaatgatt tcaatgattt caatgatgaa atgcgtaaac tcgcgccgct
    30841 ttctgccgct ctggagcaaa gcgatctggc aatcagctgg tatatgtttc acatcaaagc
    30901 gctggtggcg atcctcaatg gcgacatcaa ccgttacgcc gaacaggtgg cgacgctctc
    30961 cggtcagcgt gtggcgcaaa gttaccagga tttgcgctcc ggcgagaggg tgatcatgat
    31021 tttcgccctg ttagcggtgg tgatcaccgc gtttgccggc tggtatatct atcgcaacct
    31081 cggcactaac ctgacggcga tttcccgcgc catgagccgc ctggcgcacg gcgaagcgga
    31141 cgtcactttt ccggcgttac agcggcgtga tgagttgggt gaactggcgc gcgcctttaa
    31201 cgtttttgcc cgcaataccg cgtcactcga acgaacctca cggctgctaa aagagaagac
    31261 cacgcagatg gagattgccc aaactgagcg gcaggggttg gaagaggcgc tattacacag
    31321 ccagaaactg aaagctgtcg ggcaactgac gggcgggctg gcccatgatt ttaataatct
    31381 gctggcggtg attattggca gcctggagct ggtgaatccg gactcgccag acgcgccgcg
    31441 aatacagcgg gcgctgcaag ctgccgaacg tggggcgcta ctgacacaac gactgctggc
    31501 gttttcccgc aagcagtcct tacatccgca agccgttgaa ctgaaaacat tgctggaaga
    31561 gttaagcgaa ttaatgcgcc actccttacc ggcaacgctg gcgctggaga ttgaagcgca
    31621 gtctccggcg tggcccgcgt ggattgacat cagccaactg gaaaatgcca tcatcaacct
    31681 ggtgatgaac gcccgtgacg cgatggaagg gcagacggga atcatcaaaa ttcgtacctg
    31741 gaatcagcgc gttactcgca gcagtgggca gcgtcaggac atggtggcac tggaggtcat
    31801 cgatcatggc tgcggtatgt cgcaggcggt gaaaagccag gtatttgagc cattctttac
    31861 caccaaacag acgggcagcg gcagcgggct gggtttgtcg atggtctatg gttttgtgcg
    31921 ccagtccggt gggcgcgttg aaattgaaag cgcgccgggg caggggacaa cggtcagatt
    31981 acagcttccc cgcgcgctga cggcggcgaa aaacctatcg ccagcggcag tagaacaggc
    32041 ttctgtcagc ggcgataaac tggtgttagt gctggaagat gaagccgcgg tgcgccagac
    32101 catctgcgaa cagttgcacc tgctgggcta tttgacgctg gaagcaagca gcggcgaaca
    32161 ggcgctggat ctgctggcgg catcagcaga aatcgatatt tttatcagcg atttaatgct
    32221 ccccggcggc atgagcggcg cagaagtggt caatgcggcc cgcaaacttt acccgcacct
    32281 gacgctgcta ctcatcagcg gtcaggattt acgccccagc cataaccccg cgctgccaga
    32341 cgtggcgctg ttgcgcaaac cctttacccg tgcgcagttg gcgcaggcgc tggggcaaga
    32401 gaattgagaa tcctcctcta ctcgtaacag gcacctttgc ctaccttgta ttaactttgt
    32461 tgcgaggccg agtttatgaa acgccactcc acgctttttc ttttcaccct gctcacgctg
    32521 accaccgtac cggcccaggc cgatattatc gatgacacca ttggcaatat tcagcaggcg
    32581 attaacgacg cttctaaccc tgaccgtggg cgagattatg aagattcgcg cgacgacggc
    32641 tggcagcgtg aagtgagcga cgatcggcgc agacaatatg acgaccgccg tcgccagttt
    32701 gaagatcgtc gcaggcagct ggacgatcgc cagcaccagc ttaatcagga gcgtcggcag
    32761 ctagaagatg aagagcggag aatggaagat gagtatgggc aatgatgttg ggtatggggc
    32821 aggcatcgcc cctctgggga gagggttagg gtgagggggc cagcacgcac attccctctc
    32881 atcacgccac cccaatctcc atcccatcaa accccgcctc aaaccccgac ggcagtgcgt
    32941 tttccatcag ccacgcatca aactggtggc tgatatgggt cagaaccacg cgcggcgagc
    33001 ggataacctg attcagcgca agcacggtat ttaaatcaca gtgattacgc ggtgcatcct
    33061 cgcgcggcgg gtggctgcaa tcaatcacca ttacctgcgg ctgattattg cgtaaaaatt
    33121 tcagcgtttt ttccggcaag ccagcggtgt cagacagcca cgccacccgg ctgtgcgccg
    33181 tttccagcag ataaccgaag gtcagttttg agtggttgag cggcaggggt gtgacctgta
    33241 acccctgcaa atcaaacacc acaaacggtt ccaccgtgtg gctgaaatca agcaggcccg
    33301 gatgtttaaa cagatcgtcg cagccctgtt catccggtgg gccgtaaacc gggatcggat
    33361 cgcccacgcc ccagcgcagc gggaatagcc cctgcacgtg atccatatgg tagtgcgtca
    33421 gcaaaaactg ctggaacgat ccgggcgacc agcgatcggc gagatcgtgc cgcccggcgt
    33481 cgatcagggt gatcgcctcg ttaaacttca ctacgccgct gcatggctgg cggcgatagt
    33541 gcggcgagcg ccgcgctctg gcgcaggccg cacactcgca gccccatgcc ggaacgccct
    33601 gtgcgccgcc ggagccggtg agcgtgaggg tcaggctcat gttacagcac cttggtgaag
    33661 cggaagtggc tctgctcgta gccttcgcgc agatagaaac ggtgcgcgtc gtggcgcttc
    33721 acgttggtcg aaagttcggt catttcggcc ccggcctggc gggcttcttc ttctgcccac
    33781 gccagtaact tactgccgac gttcagaccg cgcgcctgcg gcattaccac caactcctga
    33841 atttcgccga tccagttgac atgatgcaga tgaaactgca aatgcaggcc gatcataccg
    33901 acaacttcgc catccagcag cgccagatgg tagcgcatgt tcgggtcgcg caggttggcg
    33961 ttaaagccca cgcgaaacgc gtggtggtca aactccgcct gttttagctc gcaaatcaac
    34021 gcgtaaaccg cgtcggtgtc gtactgcgtg gccgggcgaa agctcacaag caggcatggt
    34081 gtttctcctt ctgatggatt agcgtcagca gcgtgtcgac cgactggcgc aggctgccgt
    34141 cattattgag cgtatggcaa tcctgtggag tatagcgggc ggcgcgcgcc agccgggcgt
    34201 taatttcact ggcgttttcg cggccacggt tttccaggcg ctggcgcagg atctctggcg
    34261 aaacctgtaa gcaaatgggc agcagcgccg attgatagcg cgcccgcgcc tgcggcagat
    34321 gggcgcgtga gccgttgacc agcacgtcga atccagcgtg cagccagagg tcaatctcga
    34381 cgccgacgcc ataatacaga ccgttagcgt gccagcttaa ggccagcaga ttttgccccg
    34441 cgcgggtaaa aaattcctgc tcgctcaggg cgatatggtt ttcgcttccg gcgctggcgt
    34501 cgcgcgtgat gtagcgatgc gccaccagta gctgagtttg ttcccgcagg cggagttccg
    34561 ccagcaggct atctttcccg gagccggacg gccccattaa ccaaatcagt tttcccatca
    34621 tcagaacacc cttttaccct gacgccagac gtggtcgata tgaatgtgat tgcccttgcg
    34681 atgcgccagc accaggtcgg cgcgtttgcc ctcgccaatc accccgcgat cctggagatt
    34741 aagcgcctgc gccggatttt tggtcaccag cttcaccgcc tgcggcagcg taaagcggtt
    34801 gctctggtca tcggcgacgc ggaacgccgc atccagcagg ctggcggggt agtagtcgga
    34861 agagaggata tccagcaggc caagctgtgc cagttcactg gccgccacgt tgccggagtg
    34921 cgaaccgcca cgcacgatat ttggcgcgcc catcagcacg ttcatgccat gcttgcgcga
    34981 ggcttccgct gcttcaaacg tggtgggaaa ttcggcgatc acgctgccaa gctggtgaga
    35041 ttcggcaacg tgggcgtgcg ttgcgtcatc gtggctggcg agcgcaattt gtcgcgcgcg
    35101 gcacattgcg gcgatggatt cgcgattcgg ctgcgaccag cgtgcggcaa gcgccagctg
    35161 ttcctcttca tactgctgca tctgcgcatc ggtgagcgag tatttgccct gataatattc
    35221 gcgatacttc tcgcggttgg cgaactggcg ctggcccggc gagtgatcca tcagcgacac
    35281 cagcgtcacc ggctcgcgct gcaccagctt ttcaaacagc ggcagcgtgg tgttatgcgg
    35341 cagctcgcag cgcaggtgca ggcggtgctc ggcgcggttg acgccgcgtt tctgcgtctc
    35401 ttcgatggcg ttgatcatct tctccagatt ctccagccga tcgccgccgt ctcgcacgtc
    35461 gccaatcgcc acggcgtcca gtacggtggt gatgccgctc gccaccatca gcgcgtcatg
    35521 gctgctcatc gccgagtggg cgggccagtc aactttcggg cgcggggtga agaatttatc
    35581 gaggttgtct gtatgcagct caatcagccc cggcagcagc cagccgcctt cgccgtccat
    35641 cgcctccggc aggcggctct ggctttcggc aaaggcgcgg atttcgccgt cctgcatctc
    35701 cagcgaaccg tttaccactt cgttttccag caccagtttg acgttattga taatcatgaa
    35761 gaggctccca ttgggtgcag gcggtcggcg acgtcattac gtacggcttc gtcatggaaa
    35821 atgccgacga tggctgcgcc gcgggctttg gcttctcgga tcagttcgac tacggcggcg
    35881 ctgtttttgg cgtccagcga ggcggtaggt tcgtcgagca gcagaatggg gtagtcgacg
    35941 ataaagccac gggcgatgtt gacgcgctgc tgttcgccgc cggagaaggt cgatggcgca
    36001 aggcgccaca ggcgttccgg cacgttcaga cgggtgagca gacgcgcggc tttagcggcg
    36061 caggcttcac gcggtacgcc ggtatcgagc agcggctgca ttactacttc cagcgccgaa
    36121 atgcgcggga taacgcgcag aaactggctc acccagccaa cggtggtttt gcggatttcc
    36181 accactttgc gcgctggcgc agtgaccagg tctacccact cgtcaccgtg tttgatctgg
    36241 atttgccctt catcgggcag atagttggcg tacagcgagc gcagcagggt tgatttgccg
    36301 ctgccggagt ggccgtggag caccacgcat tcgcccgcgt tgacggtgag cgaggcgcgg
    36361 ttgaggacgg gcaagcgcac gccgttttgc tggtgcagga tgaaggtttt actgacgttt
    36421 tgtacgttaa tcatttttcg cctcgtggtt cgggtgtcgg atgcgacgct gatgcgtctt
    36481 atccgaccta cggggagcgc atttgtaggc cggataaggc gtttacgccg catccggcac
    36541 cggctcaatt ctgcaaaacc gatgacacca gcagctgtgt atacggatga tgcgggtcgt
    36601 cgagcacgcg gtcggttaac ccactctcca ccacttgccc ctgcttcatc accagcaaac
    36661 ggtccgccag caggcgggcg acgcctaaat cgtgggtgac aatcactacc gcgaggttca
    36721 gctccaccac caggccgcgc agcaggtcga gcaggcgggc ctgcaccgac acatccagcc
    36781 cgccggtcgg ttcatccata aacaccagct tcggatgcgt caccaggttg cgggcaatct
    36841 gcaaacgctg ctgcataccg ccggaaaagg tggtcggcag gtcgtcgatc cggttggcgg
    36901 gaatttccac ctcttccagc cacttctgcg cggtggcgcg aatatcgccg taatgacgcg
    36961 ccccggtcgc catcagccgc tcgccgatat tgccgcctgc cgagacctgg cggcgcaggc
    37021 cgtcgagcgg atgctgatgc actacgcccc attcggtgcg cagcaggcga cggcggtcgg
    37081 cctcgctcat gccatagagg gaacggtttt catagtgaat ctccccctgc tgcggcgtca
    37141 gacgcgcgga aattgatttc agcagcgtgg tcttcccgga gccggattcc ccgacaatgc
    37201 ccagcacttc ccccggccat aaatcaaaag agacatcgct aaagcctttg cccggcgcgt
    37261 aaaggtgggt caggttattg accgaaagta acggttgatt cattggcttt ttgcctcgct
    37321 ctgttggcgg caataatcgg tatcggagca gacaaacatg cggtttccgg cgtcatccag
    37381 caccacttca tcaagatagc tgtgggtcga tccgcagatg gcgcatggct catcccactg
    37441 ctgaacggtg aacgggtgat cgtcgaaatc gaggctttcc acgcgggtaa acggcggcac
    37501 cgcatagatg cgcttctcac gcccggcgcc gaagagttgc agggcgggca tcatgtccat
    37561 tttcgggtta tcgaatttcg ggatcggcga cgggtccatt acgtagcgcc cgttcacctt
    37621 caccggatag gcgtaggtgg tggcgatatg accgaagcgg gcgatatctt catacagctt
    37681 cacctgcatc acgccgtatt cctccagcgc gtgcatggtg cgggtttccg tttcgcgcgg
    37741 ctcgataaag cgcagcggtt cggggatcgg cacctggaaa ataatgatct gatcttcggt
    37801 cagcggcgtt tcggggatgc ggtgacgcgt ctggatgacc gttgcatcgt ccgtgcgctc
    37861 ggtggtgttt accccggtca cgcgcttaaa gaagttgcga atcgacacgg cgttggtggt
    37921 gtcatccgcg ccctggtcaa tcaccttcag cacgtcgctt tcgccaatca cgctggcggt
    37981 aagctgaatg ccgccggttc cccagccgta cggcattggc atctcgcgcc cgccaaacgg
    38041 cacctgataa ccggggatcg ccaccgcttt taagatggcg cggcggatca tgcgtttggt
    38101 ctgctcgtcg aggtaggcaa agttgtagcc gctcagatta gccatggttc tgctcctgtt
    38161 gcagacgttt gagtagctcc agttcggcct ggaaatcgac gtagtgcggg agtttgaggt
    38221 gcgagacaaa gcctgcggct tcgacgttgt cggcatgtgc cagcacgaac tcttcatcct
    38281 gcgccgggcc tgtcgcgtgc tcgccgtact ccggggcttg cagagcgcgg tcgaccagcg
    38341 ccatcgccat cgctttgcgc tcgctcatgc cgaacaccag cccgtagccg cgcgtgaagt
    38401 gcggagattc atccggtggg tcgataaaac cgttgaccat ttcacactca gtcatcagca
    38461 gttcgccgac gttcaccgca aatcccagct cttcgggcac aatcgacacg tcgatgtaac
    38521 cactgcggat ttcacccgcg aacgggtgat tgcgcccgta accgcgttgg gtggagtagg
    38581 ccagcgccag caaatagcct tcgtcgccgc gcatgagctg ttgcaggcgg gaagaacgtg
    38641 agcaggggta aaccggtggc gtgcgggtga tgtcatccgg ctgtgcgccg ctatcctctt
    38701 caaacttcgc cagcccctga cgcgccagca ggctgaaaac gtgcggcgac ggctgctgtt
    38761 cgctgtcggc ggtggtcagc gtcggcgctt cgccgtttgc cagcagggtg aaatcgagca
    38821 ggcgatgggt gtagtcgtag gttgggccaa gcagctggcc gccgggaata tctttataaa
    38881 ccgctgaaat acgccgttcg agacgcatct ccgtggtgtc gagcggctcg cttaccgcga
    38941 gcttcgccag cgtggtgcgg taggcgcgca gcaggaaaat tgcttcgacg ttatcgccgc
    39001 tggcctgttt cagcgccagc gccgccagct cgcggtcggc aatgccgcct tcggtcatca
    39061 cgcgatccac cgcaagatta agctgctgtt caatctgggc gacgctcagt tcaggcaaat
    39121 cggtatcgcc ccggcgtcgg ctctcttgca gggcgtgggc ggcgtcgatc gccttctcgc
    39181 cccctttcac ggcaacgtac atcagcacac ctccacatga gtggttcgcg gaacagccag
    39241 caggcgctcg ccgcaggtca ggatcaggtc gatgccgagc gggaacgggt gcgggcgctc
    39301 ggtgagttcg tgcagaatgc actccggcag ctgcggggcg atcattcgtt cttcggcaat
    39361 acccgcgccg gtgagacgca gcatgcgccc gccgctcagg ctggcgacct gtaaaatcag
    39421 cgttgcgccc gcttccggcg caacggcggt gccggtggaa agggcgttaa gctgttcgct
    39481 ggaaatcgcc tcatccgtta ccgcgaaggt cgcctgttcc ggctggctga ccagcggcgc
    39541 gttggtatga aaacgcaggc tctggctgac gatatcgttg ctcaatggcg cggcaagcca
    39601 caccggcgtg tcgttatcgg ccagcgtcag cagcacgctg gtggtggcga tattcagcgg
    39661 ctgccagccg cgtttgagct gatgcagggc gacaatcacg cccggctcgc tcatggcctt
    39721 taacaggcga cgaaaactgt gctgggcatc ctgcacggga agcataaaag cggtttccag
    39781 ggtcatgcgt tgtctccgcg aaccatcgta aagaagtcga cccggctggc gttcacttcg
    39841 gcctggcgtg cggcaatgcg tgccatacgg tcagcgtcca gcggggcaat aagcgtttct
    39901 gataagtttt gaaaatggcg ggtttgctgc atcagcgcgt caatcagcgc gcagcgttcg
    39961 gcgtgctgtt tgtcgcgccc cagcacccag ctgtagccga gggtgccgtc agtcagacgt
    40021 acggcggcgc gggtcagcgt ggcgtcgccg gcaaaaaagc gttcgccggt gccgcccatc
    40081 cgcgcctgaa tctgtaccag gccagtttca gcggcgcgga tcacctcata gttggcggtg
    40141 atgttcagcg cgttcaggcg ggctgccagt tctgccggtt ggctgtgtgc cagtacggac
    40201 atccagtgct ggcgggtcgc ggtatctgcg tgcattcagt gctccatagt gaattcaatc
    40261 atgtcggcgc gcgtcaggct gacggagtac tccgccgggc tgctttcacc gtcacggtgg
    40321 ttaagggtgc gcacgcacag cagcggggac atattcggaa tttcaagacg ctggcactct
    40381 ttggcctggg cgcggcgggc gctgatccgc gtctggctgc ggcgcagtgc aatccccgtt
    40441 tgctcgcgca gaaaatcgtg cagcgagccg ctgtcgaagc gttgcagcgt cggccagagg
    40501 gtgaggtccg cgaagtagtg gtcgattaaa cagagcgcga tgccgttgac ccggcgcagg
    40561 gtgcgcagat ggatgacgtt ctccccctcg gttatcccca gcgcgtcagc gacgtgaccg
    40621 gaggccgggc gcagcacaga cagcagtttt tcgctggtgg gatggctgcc ctgatccagc
    40681 agattctggc taaaacgcgc ctgggcgttg agcgggtaat cgaacgggcg catcagcacc
    40741 agcacgccga cgccctgacg gcgctgtacc cagccttttt ccaccagttg gtcgatggcg
    40801 cggcgcaggg tgtggcgatt tacctcaaag cgcgccgcca gctgctgctc ggcgggaaga
    40861 tagtcgccgc agcggtagtg ttgacgaagc tcctgctcaa gttttgcggc tatctcctga
    40921 tagcgtgttg ggtagctggt cggatgtgta gacaagtgca tagctatcaa tgcctcgcta
    40981 atcagataaa gtgcttacgc aaccgttgag agaggaaatc cagcaggctg accgtgacga
    41041 taatcagcac catcagggcg caggtttgct ggaactggaa accgcgaatc gcttcccaca
    41101 gcgtgacgcc gatcccgccc gcgccgacca tgccgacgac ggtcgccgag cggacgttgg
    41161 attcgaagcg atagagggag taggagatca gcagcggcat cacctgcggc agcacgccgt
    41221 agaggatctc ttcgagcttg ttggcgccgg tggcgcgaat gccttccacc gggccgggtt
    41281 caatcgcttc caccgcttcg gaaagcagtt tggagagcac accggtggtg tggataaaca
    41341 gcgccagcac accagcgaat gggccgaggc cgacggcgac cacgaacagc atggcgaaga
    41401 ccatttcgtt aatggcgcgg caggcgtcca tcaggcggcg cacgggctgg taaacccacc
    41461 acggcaccag gttttcggcg ctcatcaggc cgaaggggat ggagagaacc accgccagcg
    41521 cggtgcccca gacggcgatt tgcagcgtga cgagcatttc ggtgaggtaa tcctgccact
    41581 ggctgaaatc gggcgggaag aagtcggcgg cgaaggtcgc catgttgccg ccgtctttga
    41641 tcagcgtgag cggggccatt tccgcgccct gccacgagac gaccaacacg gcgagtacaa
    41701 cggcccagct cagaagcgag aaccagctgc gcttgggtgg ggcgatggtg atggtttgca
    41761 tgtttggctc cggtctgtag gccggataag gtgcttgcac cgcatccggc atcaacgcct
    41821 gcacattgtc ggatgcgaca ccggggcgtc ttatccgacc tacgatttac tgcatcgctt
    41881 tacttaccga actcatcgcg cttaacgcgt tgttcaggcg gtccaggtca tccagctgcg
    41941 cttgaatctc ggtggttttt gccagcttgt cctgctcatt cagcccttta ttgcctttca
    42001 cgctttgcat ctctttaaac agtgcgagct ggcgaatcgg caccagttgc aggtcgctgg
    42061 aggcgcggaa cggcgcccag cccaggcgtt ccagcaccgc tttctcttcc ggcgtcttgc
    42121 cgtaattcat aaagaagtcg tagatcttgt ctttggtggt ttcggaaagg tttttacgcc
    42181 agacgatcgg atcgcctggg atcagcggtg acttccagat cactttcagt tctttcagct
    42241 tctccggcgc ggaggttttc agcttgtcga ggttttcggt gttgttggtc gccacatcca
    42301 cctgcttgtt ggcgacggcc agcgcgttgg tttcatgccc ggcgttgacg gtgcgcttga
    42361 agtcactggc ggagatattg tttttggaga agacgtagta gccggggacg aggaagccag
    42421 aggtggagtt aggatcgcca ttaccgaagg tgagatcttt ccgcttcgcc agcagatcgt
    42481 tcaggttgtt gatcgggctg tctttgttga cgatcaacac gctccagtaa cccggcgatc
    42541 catccgccgc gacggtctga gcgaagactt ggccgttggc gcgatccact gcttccatcg
    42601 ccgacaggtt gccgtaccag gcgatatcca ctttgttgaa acgcattccc tggataatgc
    42661 ccgcatagtc cggggcaaag aaggcgttga ccttcacgcc cagcttcttc tccatatcct
    42721 gtaaaaacgg cgtccattgc ggtttcaggt tttgctgtga ttccgttgaa ataatgccga
    42781 aattcagcgc cttttcctgc tcttcggcgt gtgccgggct taacagggtg ctgaggctga
    42841 acatgctggt gaaggccagc gaggcaatta tcttagcgtt cattcgtttt cctcaatgat
    42901 ggggatgtca ggcagctttc gcgttctctt cgacgcggtt gatgctgcgg tagagatggt
    42961 caaaacgttc gttatcaaac tgttggctgc tgccgtcgta gaagacgtgc ccctggcgca
    43021 gggcgacgat gcgttcgcag tagcgcaggg cgtaatccac ctgatgcagc gtgacgacca
    43081 cggtgatgcc gtcgttctgg ttgatgtcgc gcagggtgtc catcacgatg cgcgccgatt
    43141 ccgggtccag cgaggcgatc ggttcgtcag cgagaatcac tttcgcctgc tgcatcagcg
    43201 cgcgggcaat cgccacacgc tgctgctggc cgccggagag cgtggaaaca cgctgatacg
    43261 caaagtgcgc cataccgacg cgggtcagcg cctgtaatgc gcgctgtttc tgctcgcggg
    43321 tgaaccaact aaaacaggtg cgccagaacg gcgtgctgcc gagcgcgcca atcagcacgt
    43381 tctccagtac gctcaggcga ttcaccaggt tgaattgttg gaagatgtag ccggtgttgg
    43441 cgcggctttt gcggatatcg cgggccaggc ggccttcgcg ctggacagtg cggcccagca
    43501 gctcgatatg gctaccggcg gatttatcgc cggtaatcaa accgcttaag tgacgtaaaa
    43561 gggtggattt gccggaaccg gacggcccaa gcagagccac catttcacca tgatggatat
    43621 tcagatcaac cgcgtgcagc gcctgatgct ggttgaaggt tttgctcagt tgttcgacgc
    43681 agataatcgt ttgcatgatg cagcctccct aaaaaagtgg ctccatcgtg gcggattatt
    43741 gtgacgattt ggttaattaa aagtgactaa cagatgaaga gttaacggga attcgatgac
    43801 agtgcgggag ggcgggcctc ccggcggatt attgcgttgg ttgttgtttg acgacattaa
    43861 tcatccacgg tacgccaaat ttatcggtga ctttgccaaa gccatgcgcc cagaaagttt
    43921 cctgccaggc catttcgatt tttccgttag cggcaaggtt atcaaaccag cgttttcctt
    43981 cttcgacctg ttgcgaatcg agcaccagcg taaaaccgga gtagctggct tttcctgacg
    44041 gtatagcatc gctcatcatg atatcgcttc cggcaatgcg cacattggcg tgggcgatgg
    44101 cggtatcggg aaattgcatt ccggaagggc agttctcggc gctgtcctgc gctgattttg
    44161 gcatttcgcc gaagctgatt ttatagagca gttcagcgcc caacgtacgt tgataatagg
    44221 caatcgcgtc ggagcagtta ccggcaaaag agaggtaggg acttaacggc atgatggtga
    44281 cctcagttaa gagaagccag ttaagtgtag ttcgaaattt atacagatga gagggaagcc
    44341 tgataagacg cgcgagcgtc gcatcaggca gtcggcactg ttgccggatg cggcgtaaac
    44401 gccttatccg gcctacggag ggtgcgggaa tttgtaggcc tgataagacg cgcgagcgtc
    44461 gcatcaggca tctggcactg ttgccggatg cggcgtaaac gccttatccg gcttaccgat
    44521 cacaatacaa tcagttcttt ttcacaaact cagatttcag tttcatcgga ccaaaaccgt
    44581 cgattttgca atcgatgtta tggtcgcctt caaccaggcg gatgtttttc actttggtgc
    44641 caattttcag catcgaagag ctacctttca ccttcagatc tttaatgatg gtaacgctgt
    44701 cgccgtcagc cagcagattg ccgttagcat ctttaacgat cagctcgtcg ctttcctgtg
    44761 caggttctgc gtcgttccat tcgtaggcac attccgggca gatgtacatg ccgttatctt
    44821 cgtaagtgta ttcggagttg cattttgggc agtgtggtaa tgacatgtgg atttcctcaa
    44881 aagtcagcag ggcaaaagcg ccaaaaaacg gcagattgcc gaaaaaggcc gcaattatac
    44941 acaaaatccc tgagtttgtc gggggtactg ctgattttag acgacgatcg ttttcatttg
    45001 aaaggaaacg c
//
LOCUS       DBFIFI010000018        43334 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.018, whole genome shotgun sequence.
ACCESSION   DBFIFI010000018 DBFIFI010000000
VERSION     DBFIFI010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43334
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.018"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..391)
                     /locus_tag="N7B39_000983"
     CDS             complement(<1..391)
                     /locus_tag="N7B39_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2421006.1"
                     /translation="MMKTITKQPILFTDVPVADLRNSMKQDLNQNLIERLWNKIRDFF
                     LDSDKQKAFKSIHKYINTLSVLNYNSALTPDPNFNIDATSDLDSYLKLDFDRLSPKQK
                     QTTLCCFWNKIASSLPEPYNSTIKHNII"
     gene            complement(622..1596)
                     /gene="yajO"
                     /locus_tag="N7B39_000984"
     CDS             complement(622..1596)
                     /gene="yajO"
                     /locus_tag="N7B39_000984"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HGU2421007.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFSRREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            complement(1651..3513)
                     /gene="dxs"
                     /locus_tag="N7B39_000985"
     CDS             complement(1651..3513)
                     /gene="dxs"
                     /locus_tag="N7B39_000985"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HGU2421008.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDETLILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            complement(3538..4437)
                     /gene="ispA"
                     /locus_tag="N7B39_000986"
     CDS             complement(3538..4437)
                     /gene="ispA"
                     /locus_tag="N7B39_000986"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HGU2421009.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKAQDLIDDARQSLKQ
                     LAEQSIDTSALEALADYIIQRNK"
     gene            complement(4437..4679)
                     /gene="xseB"
                     /locus_tag="N7B39_000987"
     CDS             complement(4437..4679)
                     /gene="xseB"
                     /locus_tag="N7B39_000987"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HGU2421010.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            4885..6333
                     /gene="thiI"
                     /locus_tag="N7B39_000988"
     CDS             4885..6333
                     /gene="thiI"
                     /locus_tag="N7B39_000988"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HGU2421011.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     DGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTG
                     QEVVEVETVNDFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFKNVKVYRP"
     gene            complement(6387..6977)
                     /gene="yajL"
                     /locus_tag="N7B39_000989"
     CDS             complement(6387..6977)
                     /gene="yajL"
                     /locus_tag="N7B39_000989"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HGU2421012.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLIRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(6940..7851)
                     /gene="panE"
                     /locus_tag="N7B39_000990"
     CDS             complement(6940..7851)
                     /gene="panE"
                     /locus_tag="N7B39_000990"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HGU2421013.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAACRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRTLRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            8019..8510
                     /gene="yajQ"
                     /locus_tag="N7B39_000991"
     CDS             8019..8510
                     /gene="yajQ"
                     /locus_tag="N7B39_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HGU2421014.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            complement(8638..10002)
                     /gene="yajR"
                     /locus_tag="N7B39_000992"
     CDS             complement(8638..10002)
                     /gene="yajR"
                     /locus_tag="N7B39_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2421015.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLRNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPANIAANEALKVRLLETEGIKEVLIAEEEHSAYVKIDSKVTNRFDVEQA
                     IRQA"
     gene            complement(10151..11041)
                     /gene="cyoE"
                     /locus_tag="N7B39_000993"
     CDS             complement(10151..11041)
                     /gene="cyoE"
                     /locus_tag="N7B39_000993"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HGU2421016.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(11053..11382)
                     /gene="cyoD"
                     /locus_tag="N7B39_000994"
     CDS             complement(11053..11382)
                     /gene="cyoD"
                     /locus_tag="N7B39_000994"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HGU2421017.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(11382..11996)
                     /gene="cyoC"
                     /locus_tag="N7B39_000995"
     CDS             complement(11382..11996)
                     /gene="cyoC"
                     /locus_tag="N7B39_000995"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HGU2421018.1"
                     /translation="MATDTLTHATVHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(11986..13977)
                     /gene="cyoB"
                     /locus_tag="N7B39_000996"
     CDS             complement(11986..13977)
                     /gene="cyoB"
                     /locus_tag="N7B39_000996"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HGU2421019.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(13999..14946)
                     /gene="cyoA"
                     /locus_tag="N7B39_000997"
     CDS             complement(13999..14946)
                     /gene="cyoA"
                     /locus_tag="N7B39_000997"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HGU2421020.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGFATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAAFDQWVAKAKQSPNSMSDMAAFEKLAAPSEYNQVEYFSNVKPNLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            complement(15406..16881)
                     /gene="ampG"
                     /locus_tag="N7B39_000998"
     CDS             complement(15406..16881)
                     /gene="ampG"
                     /locus_tag="N7B39_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HGU2421021.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTAYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(16925..17503)
                     /gene="yajG"
                     /locus_tag="N7B39_000999"
     CDS             complement(16925..17503)
                     /gene="yajG"
                     /locus_tag="N7B39_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGU2421022.1"
                     /translation="MFKKILFPLVALFMLAGCARPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            17808..18125
                     /gene="bolA"
                     /locus_tag="N7B39_001000"
     CDS             17808..18125
                     /gene="bolA"
                     /locus_tag="N7B39_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HGU2421023.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            18469..19767
                     /gene="tig"
                     /locus_tag="N7B39_001001"
     CDS             18469..19767
                     /gene="tig"
                     /locus_tag="N7B39_001001"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HGU2421024.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            20012..20635
                     /gene="clpP"
                     /locus_tag="N7B39_001002"
     CDS             20012..20635
                     /gene="clpP"
                     /locus_tag="N7B39_001002"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HGU2421025.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            20761..22035
                     /gene="clpX"
                     /locus_tag="N7B39_001003"
     CDS             20761..22035
                     /gene="clpX"
                     /locus_tag="N7B39_001003"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HGU2421026.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            22223..24577
                     /gene="lon"
                     /locus_tag="N7B39_001004"
     CDS             22223..24577
                     /gene="lon"
                     /locus_tag="N7B39_001004"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HGU2421027.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILNTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            24768..25058
                     /gene="hupB"
                     /locus_tag="N7B39_001005"
     CDS             24768..25058
                     /gene="hupB"
                     /locus_tag="N7B39_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HGU2421028.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            25250..27121
                     /gene="ppiD"
                     /locus_tag="N7B39_001006"
     CDS             25250..27121
                     /gene="ppiD"
                     /locus_tag="N7B39_001006"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HGU2421029.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADLQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDVAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKENLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            27272..27643
                     /gene="ybaV"
                     /locus_tag="N7B39_001007"
     CDS             27272..27643
                     /gene="ybaV"
                     /locus_tag="N7B39_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HGU2421030.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QNKAAVPAKASDEEGSRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            27749..28147
                     /gene="fadM"
                     /locus_tag="N7B39_001008"
     CDS             27749..28147
                     /gene="fadM"
                     /locus_tag="N7B39_001008"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HGU2421031.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFHWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(28199..28894)
                     /gene="queC"
                     /locus_tag="N7B39_001009"
     CDS             complement(28199..28894)
                     /gene="queC"
                     /locus_tag="N7B39_001009"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HGU2421032.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKVLNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLK"
     gene            complement(28959..30659)
                     /gene="ybaE"
                     /locus_tag="N7B39_001010"
     CDS             complement(28959..30659)
                     /gene="ybaE"
                     /locus_tag="N7B39_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HGU2421033.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEKALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSADGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMTDATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            30759..31577
                     /gene="cof"
                     /locus_tag="N7B39_001011"
     CDS             30759..31577
                     /gene="cof"
                     /locus_tag="N7B39_001011"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HGU2421034.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGAISLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLHEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            31729..32187
                     /gene="decR"
                     /locus_tag="N7B39_001012"
     CDS             31729..32187
                     /gene="decR"
                     /locus_tag="N7B39_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HGU2421035.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            32217..33989
                     /gene="mdlA"
                     /locus_tag="N7B39_001013"
     CDS             32217..33989
                     /gene="mdlA"
                     /locus_tag="N7B39_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HGU2421036.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDVLAQQSGWYRD
                     MYRYQQLEAALDDAPENREEAVDA"
     gene            33982..35763
                     /gene="mdlB"
                     /locus_tag="N7B39_001014"
     CDS             33982..35763
                     /gene="mdlB"
                     /locus_tag="N7B39_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HGU2421037.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            35944..36282
                     /gene="glnK"
                     /locus_tag="N7B39_001015"
     CDS             35944..36282
                     /gene="glnK"
                     /locus_tag="N7B39_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HGU2421038.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            36312..37598
                     /gene="amtB"
                     /locus_tag="N7B39_001016"
     CDS             36312..37598
                     /gene="amtB"
                     /locus_tag="N7B39_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HGU2421039.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(37647..38507)
                     /gene="tesB"
                     /locus_tag="N7B39_001017"
     CDS             complement(37647..38507)
                     /gene="tesB"
                     /locus_tag="N7B39_001017"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HGU2421040.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            38725..39297
                     /gene="ybaY"
                     /locus_tag="N7B39_001018"
     CDS             38725..39297
                     /gene="ybaY"
                     /locus_tag="N7B39_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HGU2421041.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(39330..39641)
                     /locus_tag="N7B39_001019"
     CDS             complement(39330..39641)
                     /locus_tag="N7B39_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HGU2421042.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            39804..39900
                     /gene="ffs"
                     /locus_tag="N7B39_001020"
     ncRNA           39804..39900
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="N7B39_001020"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            40020..40373
                     /gene="ybaA"
                     /locus_tag="N7B39_001021"
     CDS             40020..40373
                     /gene="ybaA"
                     /locus_tag="N7B39_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HGU2421043.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(40415..41965)
                     /gene="pdeB"
                     /locus_tag="N7B39_001022"
     CDS             complement(40415..41965)
                     /gene="pdeB"
                     /locus_tag="N7B39_001022"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HGU2421044.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGADCSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASSKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(42129..42599)
                     /gene="ylaC"
                     /locus_tag="N7B39_001023"
     CDS             complement(42129..42599)
                     /gene="ylaC"
                     /locus_tag="N7B39_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HGU2421045.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(42715..43266)
                     /gene="maa"
                     /locus_tag="N7B39_001024"
     CDS             complement(42715..43266)
                     /gene="maa"
                     /locus_tag="N7B39_001024"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HGU2421046.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     ALGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
BASE COUNT        10849 a        10743 c        11528 g        10214 t
ORIGIN      
        1 aaataatgtt atgtttgatg gtactgttgt atggctctgg caatgaggat gcaattttat
       61 tccagaaaca acaaagcgtg gtctgttttt gtttgggcga gaggcgatca aaatccagct
      121 ttaaatatga gtctaaatct gacgttgcat ctatattaaa gttgggatct ggagttaacg
      181 ctgaattata atttagaact gacaatgtat tgatgtattt atgaatcgat ttaaatgcct
      241 tttgtttatc actgtccaga aaaaaatcac gaattttgtt ccacagcctt tcaataagat
      301 tttgatttaa atcttgcttc attgagtttc ttaaatctgc aaccggtaca tcagtaaaca
      361 ggatcggttg tttggtgatg gttttcatca tgattgtaat cctcttacgt tgactaaata
      421 agacagtttt aggggatatt acctggagaa actatcaaaa atcgctcaaa gggatgaaga
      481 cgatgaatag ctgcaaactc attcacgaag aatttctctt gcagaggtgg attatggtta
      541 cagaaggtga tgggaggtac gttgtcccca tcatcgcaga cgatggggaa ttcagcagat
      601 aaaatgggtg aaaagcgaaa tttacttaaa tcctacgacc ggatgcggtt tatacggtgt
      661 ttccagttca gcaatctgtt ccggcttcaa agtgatatcc accgcgttca gcagctcatc
      721 aagctgttct tcccgcgaag ttccgataat cggtgcggca atgcccggtt tactcaacaa
      781 ccaggccagc gcaacttgtg ctcgtgttgc accgagttct tcactgacgc ccgttaaccg
      841 ctctgcgatc tgcgcgtcat tttcatcgct ttctttatag agatttttcc ccacctcatc
      901 agacaccagt cgtgcggtag tttctcccca cggacgcgtc agtcgccccc gcgccagcgg
      961 actccacgga atgaccgcca cgccttcctg ataacacagc ggcagcatct cgcgctcttc
     1021 ttcgcgataa atcagattgt agtgatcctg catactgaca aactgcgccc agccgtgctg
     1081 tttttgtagt tccagtgcct gagcaaactg cgaagcgtgc atggatgacg cgccgatata
     1141 acgcgctttc ccggctttta ccacgtcgtt gagggcttcc agcgtctctt cgatcggcgt
     1201 gttgtaatcc cagcgatgaa tttgcaggat atcgacataa tccatgccga gacgtcgcag
     1261 gctgtcgtcg atagagcgca aaatttgtgc acgggataat ccttccggta aatcaccaac
     1321 gcgatggaac actttggtgg caacgaccac gtcttcacga cgggagaaat cccgcagtgc
     1381 gcgaccgacg atctcttcgc tgctgccatc ggaatagcta ttggcggtat caaagaaatt
     1441 tatgccgcct tccagtgcgc gtttaatgat gggacggctg ctctcttccg gcagtgtcca
     1501 tgcgtgatta ccacgatctg gctcgccaaa ggtcatacag ccgaggcaaa gtcgggaaac
     1561 gcgaaggtcg gtttttccta aggggttgta ttgcatgctg ccactcctgc catgaataat
     1621 ttcttaagca tagcaggagt ggagtaggga ttatgccagc caggccttga ttttggcttc
     1681 cataccggcg gcatcgaggc cgagttcggc gcgcatttct tcctgagttc cttgcggaat
     1741 aaagaagtcc ggcaggccaa tgttcagcac gggtactggt ttacgatggg ccatcagcac
     1801 ttcgttcact ccgctgcctg caccgcccat aatggcgttt tcttctacgg taaccagtgc
     1861 ttcatgactg gcggccattt ccagaattaa cgtttcatca agcggtttca caaaacgcat
     1921 atcgaccagc gtggcgttca gcgattcggc gactttcgcc gcttctggca tcagcgtacc
     1981 aaagttaagg atcgccagtt tctcgccacg acgcttcaca atgcctttgc caattggtag
     2041 tttttccagc ggcgtcagtt ccacgccgac cgcgttgcca cgcgggtagc gcaccgctga
     2101 cgggccatcg ttatagtgat agccggtata gagcatctgg cgacattcgt tttcatcgct
     2161 cggggtcata atgaccattt ccggtatgca gcgcaggtaa gagagatcaa aagcgccctg
     2221 gtgggtttga ccgtcagcac caacaatgcc cgcgcggtcg atggcgaaca ggaccgggag
     2281 tttttgaatt gccacgtcat gcagcacctg atcataggcg cgttgcagga aggtggagta
     2341 aattgccacg atcggtttgt agccaccaat cgccagaccg gcggcaaagg tcaccgcgtg
     2401 ttgctcggca atcgctacat caaaatagcg atccgggaat ttacgtgaaa actcgaccat
     2461 gccggaacct tcacgcatcg ccggagtaat cgccatcagt ttgttatctt tcgctgccgt
     2521 ttcgcacaac cagtcgccaa agatttttga atagcttggt aaaccaccgc tacttttcgg
     2581 caaacaaccg ctggagggat caaatttagg cacggcgtgg aaggtgattg ggtctttttc
     2641 tgccggttca taaccacgac cttttttggt catgatatgc aggaactgcg ggcctttcag
     2701 gtcgcgcatg ttcttcagcg tggtgataag ccccagcaca tcgtgaccgt caaccgggcc
     2761 gatgtagtta aagcccagct cttcaaacaa cgtgccaggc accaccatgc ctttaatatg
     2821 ttcttcggta cgtttgagca gctctttaat tggcggaacg ccagagaaaa cttttttccc
     2881 gccttcgcgc agtgaagagt aaagcttacc ggaaagcagc tgcgccagat ggttgttaag
     2941 cgcgccaaca ttttcggaaa tcgacatttc attgtcgttg aggaccacca gcatatcagg
     3001 acgaatatcg cccgcgtgat tcatcgcttc aaacgccatg cctgcggtaa tcgcgccgtc
     3061 gccaatcaca cagacggtgc ggcgattttt gccttctttc tcggcagcaa ccgcaatacc
     3121 aattccggca ctgatggagg ttgatgaatg cccgacgctt aatacgtcat attcgctttc
     3181 gccgcgccac gggaacgggt gtagaccgcc tttctgacgg atggtgccga ttttgtcgcg
     3241 gcgtccggtc aaaattttat gcggataagc ctgatgcccc acatcccaaa tcaattggtc
     3301 aaacggggtg ttatagacat agtgcagcgc cacggtcagt tcgaccgtgc ccagcccgga
     3361 ggcgaagtgc ccgctggaac ggctcacgct gtcgagtaaa tagcggcgca gttcgtcgca
     3421 gagtttcggt aaactctctt ttggcaacag tcgtagctcc tgggtggagt cgaccagtgc
     3481 cagggtcggg tatttggcaa tatcaaaact catcaggggc ctattaacac ttattgttta
     3541 tttattacgc tggatgatgt agtccgctag cgcttccagt gccgaagtat cgatcgactg
     3601 ttcagccagt tgtttcagcg actggcgggc atcgtcgatc agatcctggg ctttcttccg
     3661 ggcttgctca agacccagga gtgcagggta ggtacttttg ccaagctgct ggtcggcacc
     3721 ctgacgtttt cccaacgttg cagtatctcc caccacatcc aggatgtcat cctgaacctg
     3781 gaaggcaagg ccgatgctct ctgcgtactt gtcgagtact ggtagagcac gacgtccttt
     3841 atctccggcg cttaatgcac caaggcgaac ggcggcgcga attaatgcgc ctgttttatg
     3901 acgatgaata cgctcaagcg cgtccagagg tacgtgtttg ccttccgcgt ctaaatccaa
     3961 cgcctgacca ccacacattc cggcaatacc gctggcgctc gccagttcag aaatcatcga
     4021 aattctgtcg cgatccgaca cttctggcat atcagcatcg cttaaaatcg agaacgccag
     4081 cgtttgtaaa gcgtcgccag cgagaatcgc gttcgcttcg ccaaacttca catggcaggt
     4141 cggcaagccg cgacgcagat cgtcatcatc catcgccggt aaatcatcat gaattaatga
     4201 gtaagcgtgg atacactcta cggcggcagc gggtgcgtcc agcgtgtttg tgctaacgcc
     4261 gaacatatga ccggtggcat aaaccaggaa aggtcgcagg cgcttaccac ctaataatgc
     4321 gccatactgc atggtttcga ccacgggagt gttctgaaag ggcagtgggg cgataaaacg
     4381 gctcagcgcc tggttggcct gcttaacgca ggcttcgagt tgctgcggaa agtccattac
     4441 tcattgtccg gtgtaaaagg ggttagagag gcgtcttcgt tgtcagacag cagaatttgt
     4501 acgcgctgtt cggcttgttg taatttggcc tgcccctgac gtgccagctg cacgccgcgt
     4561 tcgaactcgt tcagcgcctc ttccagcggc aggtcgccac tttccagacg ggttacaatc
     4621 tgttccagct cgctcagcgc cttttcaaag ctggcgggcg cctcattttt cttcggcata
     4681 atgaatttct gactctcaat atttttcgcc ccgtcatggt aacggactca gggcaaatag
     4741 caaataacgc gcaatggtaa ggtgatgtgc gcagcaaagc gatgttagtg gtatacttcc
     4801 gcgcctggat gcagccgcag gtgtgggctg ctgtattttt ccctatacaa gtcgcttaag
     4861 gcttgccaac gaaccattgc cgccatgaag tttatcatta aattgttccc ggaaatcacc
     4921 atcaaaagcc aatctgtgcg cttgcgcttt ataaaaatcc ttaccgggaa cattcgtaac
     4981 gttttaaagc actatgatga gacgctcgct gtcgtccgcc actgggataa catcgaagtt
     5041 cgcgcgaaag atgaaaacca gcgtctgact attcgcgacg ccctgacccg tattcctggt
     5101 atccaccata ttctcgaagt cgaagacgtg ccgtttaccg acatgcacga tattttcgag
     5161 aaagcgttgg ttcagtatcg cgatcagctg gatggcaaaa ccttctgcgt acgcgtgaag
     5221 cgccgtggca aacatgattt tagctcgatt gatgtggaac gttacgtcgg cggcggttta
     5281 aatcagcata ttgaatccgc gcgcgtgaag ctgaccaatc cggatgtgac tgtccatctg
     5341 gaagtggaag acgatcgtct cctgctgatt aaaggccgct acgaaggtat tggcggtttc
     5401 ccgatcggca cccaggaaga tgtgctgtcg ctcatttccg gtggtttcga ctccggtgtt
     5461 tccagttata tgttgatgcg tcgcggctgc cgcgtgcatt actgcttctt taacctcggc
     5521 ggcgcagcgc atgaaattgg cgtgcgtcag gtggcacatt atctgtggaa ccgctttggc
     5581 agctcccacc gcgtgcgttt tgtcgctatt aatttcgagc cggtagtcgg ggaaattctc
     5641 gagaaaatcg acgacggtca gatgggcgtt atcctcaaac gtatgatggt gcgtgccgcg
     5701 tctaaagtgg ctgaacgtta cggcgtacag gcgctggtca ccggcgaagc gctcggccag
     5761 gtgtccagtc agacgctgac caacctgcgc ctgattgata acgtctctga tacgctgatc
     5821 ctgcgtccgc tgatctctta cgacaaagag cacatcatca acctggctcg ccagattggc
     5881 accgaagact ttgcccgtac tatgccggaa tactgcggtg tgatctccaa aagcccgacg
     5941 gtgaaagcgg ttaagtcgaa gattgaagcg gaagaagaga aattcgactt cagcattctc
     6001 gataaagtgg ttgaagaagc gaataacgtt gatatccgcg aaatcgccca gcagaccggg
     6061 caggaagtgg tggaagtgga aaccgtcaat gacttcggcc cgaacgacgt gattctcgat
     6121 atccgttctg tcgatgaaca ggaagataag ccactgaaag tcgaagggat tgatgtggtt
     6181 tcactgccgt tctacaagtt gagcaccaaa tttggcgatc tcgaccagaa cagaacctgg
     6241 ctactgtggt gtgagcgcgg ggtaatgagc cgcctgcagg cgctctatct acgcgaacag
     6301 ggctttaaga atgtgaaggt gtatcgtccg taatttgtgg tttttacgtc gcatctggtc
     6361 agatgcgacg tttgccgcat ccgacactac tcgtaataat tataaatccc tgccgccatc
     6421 accagttgtg atgccacttc atgggctttc tcgcgcccaa ccagcaggtc gataattttc
     6481 agaccaaagt cgatagctgt acccggcccc tggctggtca gcaattttac ccgtgcatcc
     6541 cagacgacgc gcttgtcctg ccattgttca gcgggaattt tgtctttcag cgtcgggaag
     6601 ccggtcatat tgccaatcgg aaagatatcg tgcggcacca gcacggtggc tggcgcggcg
     6661 caaatagccg cgacgatacg cccggaacgg tggaactgtt taacggtttc aaccagcagg
     6721 gtactatcgc gaaaacactc cgcgccttta atgccaccag gcagcacgat cacgtcatat
     6781 tcgccatcag ccacttcgac cagcggcgca tccgccagca gcttcacgcc gcgcgagcag
     6841 gtaatcgcca ggttgccatc gctggctacg ctggcagtgg tgactttgat accgccgcga
     6901 atcaagaggt cgatcgtggt gacggcttca gtctcttcac taccaggggc gaggcaaacc
     6961 agtgccgatg cgctcatatt cactctcctt tctttttacc atttcaaaca ggcgggtgtt
     7021 ttccggtacg gcaatcccat gcgcgcgggc gcggcgtaag agaaaaccat tgatatagtc
     7081 gatttcggtg tggcgcagcg tgcggatatc ctgcaacatc gacgagatat tttccgctgt
     7141 ggcatcaatc acctgcatca cgtaatcacg taaatcttcc gctgaagtat gatgcccttc
     7201 gcgttcgatc actgccgcga cttcttcgca tatttgcata atttcttgcg gatgatgacg
     7261 taattcaccg ttcgggcaat tccagatggc agtcagtgga ttaatcacac agttgactgc
     7321 cagcttgcgc cacagctcgg cgcgaatatt gttatgccag gcaacgtcag gcaacacggt
     7381 ttgcaatata tccgccagat aactgtaatc gccgtcctgt tgccgtgctg gaccaatatg
     7441 cgtgataccg tttgccacat gaataatgac attgccgtcg cggcaggcgg catgggtggt
     7501 ggtgcccatc agtaatggct gctgaatgtt ttgcaattct tcgatggtgc ccatgccgtt
     7561 gtgaattaac agtattggcg tagttacagg cagtgtggac gcgaggcttt tgacggcatc
     7621 ggaaacctgc catgctttca gcgtcaccag gagcagatcg ctggtggcaa gaaaatcggg
     7681 atcgttggcg gtcagcgatt cgttaaatat cgaaccatca gtctcaacca gattcacact
     7741 acaataaggt tgcggtacgc gcagccagcc ctgaacttcg tgaccctgtt tacaaagtgc
     7801 tgtaagccat aattgcccta aggcaccgca tcccaatacg gtaattttca ttgttcctcc
     7861 tcacccgcaa ccactccggg tgttcaataa ggctatccct taattgtgca tgctgttgcg
     7921 actatgcaca attaagggat acgtcctggt gcaggactgt tggttattta actttgcagg
     7981 tattatgctt cgcatcaaaa atgaagggag aggaaaagat gccatctttc gatattgtct
     8041 ctgaagttga tcttcaggaa gcacgtaacg cggtcgataa cgcgagccgc gaagtggagt
     8101 cccgttttga cttccgtaac gttgaagcct catttgagct gaacgacgcc agcaaaacca
     8161 tcaaagtgtt gagcgagtcc gacttccagg tcaatcagtt gctggatatt ctgcgtgcca
     8221 agctgctgaa gcgcggcatt gaaggcagtt cactggatgt accggaaaat atcgttcata
     8281 gcggtaaaac ctggtttgtg gaagcgaaac tgaaacaggg cattgagagc gccactcaga
     8341 aaaaaatcgt caagatgatc aaagacagca aactgaaagt gcaggcgcaa attcagggcg
     8401 atgagatccg tgtgacgggc aaatctcgtg atgatttaca ggctgtcatg gcgatggtgc
     8461 gcggtggcga tctcggtcag ccgttccagt tcaaaaactt ccgcgattaa tcgcgacaac
     8521 ctgcgctttg ttcatgtcag atgcggcatg aacgcctggt tcggcctaca aagttttgta
     8581 aatcaggaac ttagtaggcc tgataagcgt agcgcagcag gcaatttcgc ctcgctttta
     8641 tgcctggcga attgcctgtt caacatcaaa gcgattcgtc actttgctgt cgattttcac
     8701 ataagctgaa tgttcttctt ctgcaatcaa cacttctttg atgccttcag tttccagcaa
     8761 acgcactttt aacgcctcgt ttgcggcaat gttcgccggg atttcaatgc gcaaactgct
     8821 gacatacggc ggttctttca tggtactggc gactgccagc cacactgcgg ccagcattgc
     8881 gccagcgaga aataccccct gaccgtcaaa catgccgtca atccagccgc ccagcgaacc
     8941 gccaatcgcc acgccaagaa actggctggt ggagtaaaca cccatcgccg tacctttgta
     9001 acctgctggc gactctttac tgataagtga gggcaggagg gcttccatca aattaaacgc
     9061 cacaaagaaa agctgcacgc cgaccaccag ttgccagaac tgcgtttgcg cgttcctcaa
     9121 cacaatttcc gcaaccacga tcaacccgac gcagaagaca aagacttgct tcattttgcg
     9181 cttaacttca gcgtagataa tgaaaggcac gaccgagcca aaggcgatta gcattgtcgc
     9241 cagatagacc ttccagtgtt cagccgccgg gaaccccgca tcagccagtt gtccgggcag
     9301 ggcaacaaac gtcgacatca gcaaaatatg cagacacata atgccaaagt tgagtttcag
     9361 cagccgcggt tccgccagca ccttactgaa actgcctttc accattccgg actcacgatt
     9421 aagtacgtga gtgctactgt tgggcacaac ccaaatggtc aacgcaatgc cggtcgttgc
     9481 cagaatagcg atcatccaga acagcgcgtg cagcccaagt ttgtgagtga tgatcgggcc
     9541 aagcaccatc gcaatggcaa aggtaatgcc aaagctcacg ccgataaacg ccattgcttt
     9601 ggtgcggttt tgttcgcgcg tgagatcgga aagcagcgcc ataacggcag cggcaatcgc
     9661 accggagcct tgtagcgccc gacccagaat aattccccag atggagtcag agagcgcagc
     9721 gataacgcta ccggcggcaa acactgccag cccaccgaca attaatggtt tgcgaccaat
     9781 gcggtcggaa agcaggccaa acggaatctg aaaaacggcc tgagtcagac cataaatacc
     9841 aatggcaata ccgattaatg cttcgctggc accttgcaga gccatgccgt acgtggtcag
     9901 aaccggcaga accatgaaca tgcccagcat gcgcaacgag aatacggtcc ctaaacccca
     9961 ggtcgcgcgc cgctcacctg gcgtcatttt ataatcgttc attaccacct ctgttttaaa
    10021 ttcgcgacta gtgtaaagcg gcaaacgaat ggggtaaaca tgcggatttt ttagtaaata
    10081 tttcgagagg gatatgtttc taatgctaag aaaaaaggtg ccgtagcacc tttttaatag
    10141 agaggttgtg ttaccacaca gcagccagca gcgtatgcga gtccggcacc ataaaatcaa
    10201 cggacatcat caccgagagg gcagtgatgg cgatgataga gaagccgaac agcttgcgcg
    10261 cccagattct gtcatcagca actttataac cgcgcagagc catacctaac caccagacgc
    10321 taaccgccgc ggcgaccacc agatatttat atccagcgta accgccaaga gagagcatca
    10381 gcgtggcaac ggcaaaggcg atgatataca gcgtgatgtg attcttcgcc accgaaatgc
    10441 cttttaccac tggcaatacc ggaatgtttg ccgcctggta atccttaaag cggaaaatgg
    10501 cgatggcata ggagtgaggc atctgccaca ggctgaagat agccagcagg atcgctgcgc
    10561 cgctatcgaa ctcgccggtt actgcacagt agccaatcac cggcggcgca gcgccggaga
    10621 gcgaaccaat caatgtgccg tagacggagt ggcgtttcat gtacaggcta taaacgccga
    10681 cataaaccac aaagcccatc acccccagcc agcaggccag cggattcgcg ccaaaccaca
    10741 gcagcataaa gccagcaata cccagcaagg tggcgtacac cagcgagaca gcaggagaga
    10801 tcaggccttt aaccagcacc cgattcttcg tcctttccat ctttctgtcg atatccctgt
    10861 cgatgtagtt gttaaacaca caacccgacg ccacaaccag tgacacccca accagcgtgt
    10921 agataaacag gggataatca atgctgccct ttgaggccag caggaatccc ccaatcaccg
    10981 agatcaggtt gccaaagatg atgcctggtt tcgttacttg caggtattgc ttaaacatca
    11041 taaccgccgc tcttagtgca tcatcatgtt gtagttgagg ttccacataa tccagatgga
    11101 gcctacaact aggatagcga tgattagcac ggtgaagaca aacgccgtca tgttccagcc
    11161 ttcatctgat ttggtattca tgtgcaggaa gcacaccaga tgcaccagaa cctgtaccac
    11221 tgccattgcc aggattgttc ccagaattac ggccggagag gcagcccctg tcatcaccat
    11281 ccagaacgga atcaccgtca gaatgatcga caggataaag cctgtcatgt aggtttttac
    11341 gctgccatgg gacgcgccgc tgtgatcggt agaatgactc attacatcgc ccccatcaga
    11401 taaacaacag tgaacacaca gatccaaacc acatccagga agtgccagaa caggctcagg
    11461 cacatgatgc gggtacggtt agtgctggtc aggccgcgac gagcgatttg taccatcagc
    11521 accgccatcc agataagacc ggaagtgacg tgcagaccgt gcgtgccgac cagcgcaaag
    11581 aacgctgaca ggaagccgct gcgatccgga cccatgccgt taacaatcag gtgatggaat
    11641 tcgtagattt ccatcccgat aaatccggcg ccaaacaacc aggtcaacgc cagccaggag
    11701 ataacctggc ttttgttgtt tttgtacatg gcgatagccg ccatgccgta ggtgatggag
    11761 ctgaacaaca gcaagaaagt ttcaaccaga acgaacggca gttcgaaaat gtccttacct
    11821 gtcgggccgc ctgcggtgcc gttcaccaga acggcatagg tagcaaacaa gatagagaac
    11881 agaatgcagt cgctcatcag gtagatccaa aatccgaaga ttttggttcc gcctgcatcg
    11941 tggtgcccgt gttcgtgcgc gtggacagtc gcgtgcgtca aagtatcagt tgccattttt
    12001 cagccctgcc ttagtaatct catcgaaatg ctggttttcc agtttttcga tttctgccac
    12061 cggcacgtag taatccacgt cctcgtcgaa gcttttcacg atccaggtga tgatcatgcc
    12121 tgcgaagcca acaatcgcca gccaccagat atgccagatc atggcgaaac cgaagatggt
    12181 ggagaaagcc gcaatgacga tacctgcacc gctgtttttc ggcatatgaa tttcttcata
    12241 gtggtcaggc tttttatacg cttcgccttt ctctttcatt tcccagaatg catcacgctc
    12301 gtgaacgtgc ggcacaacgg caaagttata gaacggaggc ggggaagagg ttgcccactc
    12361 cagcgtacgg ccaccccacg ggtcgccagt caggtcacgg ttctggtcgc ggtcacgaat
    12421 agaaacgtac atctgaataa cgaggcagag aatacctagc gcaatcagta ctgcaccgct
    12481 ggctgcaatc atcagcatgg tgtggaactg cgggtcaatc tgctggctca aacgacgggt
    12541 catgcccatg aagcccagcg cgtacagtgg cataaaggca acgaagaagc cgatgatcca
    12601 gaaccaaaac gcgcgtttac cccaggtttc gttcagtttg aaaccgaacg ctttaggcca
    12661 ccagtaggtc atccctgcga agcagccgaa gaccacgccg ccgatgatca cgttatggaa
    12721 gtgcgcaatc aggaacaggc tgttatgtag aacgaagtcc gcgcccggta ctgccagcag
    12781 cacgccagtc ataccgccca ccgagaaggt gacgataaaa ccgatagtcc acagcatcgc
    12841 agaatggaac acgatgcggc cctgatacat ggtgaacagc cagttgaaga tcttcacccc
    12901 ggtcgggatg gcgataatca ttgtggtgat accaaagaag gcgtttacgt tcgcgcccgc
    12961 acccatcgta aagaagtggt gcagccaaac gatgaacgac agcacggtga tacagacggt
    13021 tgcccatacc agcgaggtat aaccaaacag acgtttacgc gagaaggttg ccgcaatttc
    13081 ggagaacaca ccgaaaacag gcaggatcag gatgtaaact tccgggtggc cccaggccca
    13141 aatcaggttg atgtacatca tcatgttgcc acccatatcg ttggtaaaga aatgggtgcc
    13201 cagatagcga tccagggtca acaacgcgac ggtaaccgtc agaattggga aggaagcaat
    13261 aatcagtacg ttcgcgcaca gtgatgccca ggtaaatact ggcatcttga acatggtcat
    13321 gcccggtgcg cgcatcttca gaatggtaac gaagaagttg ataccggtaa gcgtcgtacc
    13381 tataccggat agctggagac tccatatcca gtaatcgaca ccgactcccg gactgtactc
    13441 tattcccgat agcggtggat aggccagcca gccggtctgc gcaaattcgc ccacgccgag
    13501 agaaacgtta accagaatca caccaacaac ggtaaaccag aagcttaagt tgttgaggaa
    13561 cgggaacgca acgtcacgcg cgccgatctg cagcggaacc accaagttca tcagaccgat
    13621 aacgaaaggc atcgccacga agaagatcat aatcacgccg tgcgcggtga agatctgatc
    13681 gtagtggtga ggtggcagga agcccgcttc gcccgccgag gcaagagcct gctggctacg
    13741 catcataatg gcgtcagcaa aaccacgcag caacatcaca atcgccacga tgatatacat
    13801 gataccgagg cgtttatggt cgacggaggt cagccactct ttccacaggt aggtccactt
    13861 accgaagtaa gtgatcaggc caacgagcgc cagacctccc aaaataatgc cagcgatcgt
    13921 aaccatgacg ataggttcat ggaacgggac tgcatcaagt gataattttc cgaacatctt
    13981 tattcttcct caaccccttt aatgggcgga ttccgcgtgg ctcatgtcca tgccttccat
    14041 accttcgtgt gcgctgtgct caccttctgg ctgggtcatg tccatgctct taccgtgagc
    14101 cataaacttg ttaattacat cggcaaacaa gtttggtttc acgttggaga aatattccac
    14161 ctggttgtat tcgctaggcg cggccagttt ttcgaacgct gccatgtcag acatggagtt
    14221 cggcgactgt ttcgcttttg cgacccactg gtcgaatgcg gcgcgatccg gtgttgcaat
    14281 agctttgaac ttcatgcctg agaagcccgg gccgctgtag ctggcggaga taccgtcata
    14341 agtgccgggt tcgttggcga tcagatgcag gcgagtctgc ataccggcca tggcataaat
    14401 ctggctaccc agacgcggaa tgaagaagga gttcatcacg gagttggagg tcactttgaa
    14461 gtacaccgga gtgttcgccg ggaaagcgat ttcattcacg gtagcaaagc cctgttccgg
    14521 gtagatgaag aaccatttcc agtccatgga aaccacttcg atggtaatgg gcttctcgtc
    14581 gtgtgccagc ggcttgctag gctcaagagc gtgagtggtt ttccaggtca gtaccgcaag
    14641 gaagatgatg attaagatag gtaccgtcca gaccacagct tccactttat tggagtgtga
    14701 ccagttcggg ctgtacttag catctttatt gctcgcacgg tacttccagg cgaaaccaac
    14761 agccatcaag attgcgggaa taacgacaat caacatcagg ccaaatgccg tcagtatcag
    14821 tgaacgttgc tccagaccaa tctgtccttt gggatctaac agcgcagaat tacagccact
    14881 gagcaataca gtgcctgcaa ataatgacaa ccatcccaaa cttttattgt atttcctgag
    14941 tctcatttaa cgacctcaat tccacgggat ctggtggcgt ttaaaatgtg tgggcatttt
    15001 acgggaaggt tacattactg taaacatgat taaattcctg ttacttggat ttggctgtca
    15061 ggtcacatat gttgcaaaac atatcaggtt gattttaaga agctgttgca aaagggcgtt
    15121 ataacaaaag gggagtgcag aagaagtctg gacgggcaat tggtataatc aatgcgaaat
    15181 aaaacaatta tttaacaaat aattatcatt ggagtgacaa cttggtggtg agaaaataaa
    15241 gactcattag ctgaatcgtg taagaaaaat agaatttata aatggcgcca ataatttcca
    15301 aacgctaagc cgcacaaaag aacaaatatt aataagcgat gctgatgttt atttagccgt
    15361 aataattacg gcgagtgatt actacagcta aataatattt acagattacg tcagatgcgt
    15421 ttttcgtagc gccagataat ccagcaaacc accaagcacg acgccagaaa gggcgactaa
    15481 aaccccgact tccagcagag caggcaggaa agagaagtgc gtcaaatcca gcgcgtccat
    15541 cgtcaatagc agtaaccaca cggccaacag gctgacgccc gccgccagtg tccacatggc
    15601 aaaggcataa cctgccggat atgcggtacg ggagataaag ttgtcattta ctcgtgtata
    15661 ttcaagcgtc tggcggcaaa ccagcagcaa aataagccct ggtacggcag cggcgacgga
    15721 gaatagatag aatgtcgacc agccgtgtgc ttcaacaaac caacccgcca cggggccgac
    15781 ataaactcgc cctacagcag aaagcgctga gagcagggca aattgagtag cggaaaatga
    15841 cttattacat agcgtcatta acagcgcgac aaaggctgat gtgcccatcc cgccacagag
    15901 gttttcgaaa aagacggctg cgcccatgct gtagagatgc ttatcagtaa tcgacagcag
    15961 ccagtaacca gcgttagacg caccttgtaa aatgccgaaa atcagcagtg cccggaacag
    16021 tgacaggcgc tgcatcaaaa tcccaccgta caatgcgcca acaatggtcg ctagtaagcc
    16081 aagcgttttg tttaccacgc ctacttcacc cgcatcaaac ccgacgccgc gaatcaaaaa
    16141 cgtggttgtc aggctcatgg cgaatgcgtc gcccagctta tacagcacga taagaagcaa
    16201 aataagccag gcattattgc gaccaaagaa atctcgcaga ggtgcaacaa ccgcttgttc
    16261 cagcgttttg ggcacaggaa tggtgtcggt tggttctggt gcaagcaacg tcgcgataat
    16321 acaggggatc aacagcgccg ccatcaacca gtacatgccc tgccagccca gccatttatc
    16381 tgccagccac agggctaggc cgccggaaac cagcatccct aaacggtaac ccagaacgct
    16441 gattgccgcg cccgcaccac gttcttctgc cgggagcaca tcggttttcc acgcatcgaa
    16501 gacaatatcc tgggaggcag agcaaaaagc gatcaccacc gccagcgccg ccatccagcg
    16561 gagttgggtg cctggttcga gaaaacccat cgccgcaatg gcgactaata acaggatttg
    16621 cgtggcgagc agccaaccgc gccgccgccc aaaaaatggg ggcgtgtagc ggtccatcag
    16681 cggtgaccag aggaatttaa aaacgtaagc ctggcctacc agagagaaga aaccaatggt
    16741 tttgagatcg atattctcga ccgtcatcca ggcctgtaag gtgccggatg tcagggcgag
    16801 cggtagcccg gaagcaaaac ccaggatcag caatatggct gaacgcggct gttgaaaaat
    16861 acgtaaatat tgactggaca tgggcgtcta caggcccggc ttgcaccggg ccagagggca
    16921 gaaattaacg cgcgttctgc ttgatgaatt cgtgaatgct ggtgtcctga gacatatcag
    16981 cgatggtatc ggtcagcaca ctgttaaccg catcggcgat atttttgttg gaggcctgga
    17041 acgcaccttc aacgttgtag ctggcacgat agtttttggt cattttgttg ccattctgcg
    17101 cggtagcgat gatggcgata tccgctttgg tagcgatgtt gtagcgcacg ttgccctggg
    17161 acacgtcagc atacagctgg ctaacgatga tttgcagatt aaccgggcca ttcggaccaa
    17221 ccatgtaacc acgcgcggtc atctgttttt ccagcacttc ttgcagcagg aaacgcagat
    17281 cgcgggaggc ggtcagggta acgatttgat tatcgcgggt gacttttgcc agcgcctgat
    17341 cggtacgctg atcggcacca ttaatgctta cggtgacgcc catcaggctt ggatcctgct
    17401 gtggcagtgt aatcgtcggg gaaacttcaa tagttgttgg cggtcttgcg catcctgcaa
    17461 gcataaacag agcaactaac gggaagagga tttttttgaa catgttcggg ctctcagaga
    17521 ctcttaagcg tgtttggtaa aaattcccgc catcataaca ttgccaacgg cgaggggaag
    17581 taggtaaggc atgtaaattc atcatgttga cgaaataatc gcccctggta aaagaaacgc
    17641 tgatgcgagg cctgtgtttc aatctttaaa tcagtaaact tcatacgctt gacggaaaaa
    17701 ccaggacgaa acctaaatat ttgttgttaa gctgcaatgg aaacggtaaa agcggctagt
    17761 atttaaaggg atggatgaca tctcagcgtt gtcggaggag atatttcatg atgatacgtg
    17821 agcggataga agaaaaatta agggcggcgt tccaacccgt attcctcgaa gtagtggatg
    17881 aaagctatcg tcacaatgtc ccagccggct ctgaaagcca ttttaaagtt gtgctggtca
    17941 gcgatcgttt tacgggtgaa cgttttctga atcgtcatcg aatgatttac agtactttag
    18001 cggaggaact ctctactacc gttcatgcgc tggctctgca tacttacact attaaggagt
    18061 gggaagggtt gcaggacacc gtctttgcct ctcctccctg tcgtggagca ggaagcatcg
    18121 cgtaaaaacg catttgcaac tgtcggcgct tttccagtat gttgctaaag attttatgaa
    18181 aaacggcctg cgggccgttt tgttttgtct ggattttgcg ctttttgccc agcattcaga
    18241 cgaaaattgc ccgggaattg tgaaaaaata cgcgacagcg cgcaataacc gttctcgact
    18301 cataaaagtg atgccgctat aatgccgcgt cctatttgaa tgctttcggg atgattctgg
    18361 taacagggaa tgtgattgat tataagaaca tcccggttcc gcgaagccaa caacctgtgc
    18421 ttgcggggta agagttgacc gagcactgtg attttttgag gtaacaagat gcaagtttca
    18481 gttgaaacca ctcaaggcct tggccgccgt gtaacgatta ctatcgctgc tgacagcatc
    18541 gagaccgctg ttaaaagcga gctggtcaac gttgcgaaaa aagtacgtat tgacggcttc
    18601 cgcaaaggca aagtgccaat gaatatcgtt gctcagcgtt atggcgcgtc tgtacgccag
    18661 gacgttctgg gtgacctgat gagccgtaac ttcattgacg ccatcattaa agaaaaaatc
    18721 aatccggctg gcgcaccgac ttatgttccg ggcgaataca agctgggtga agacttcacc
    18781 tactctgtag agtttgaagt ttatccggaa gttgaactgc agggtctgga agcgatcgaa
    18841 gttgaaaaac cgatcgttga agtgaccgac gctgacgttg acggcatgct ggatactctg
    18901 cgtaaacagc aggcgacctg gaaagaaaaa gacggcgctg ttgaagcaga agaccgcgta
    18961 accatcgact tcaccggttc tgtagacggc gaagagttcg aaggcggtaa agcgtctgat
    19021 ttcgtactgg cgatgggcca gggtcgtatg atcccgggct ttgaagacgg tatcaaaggc
    19081 cacaaagctg gcgaagagtt caccatcgac gtgaccttcc cggaagaata ccacgcagaa
    19141 aacctgaaag gtaaagcagc gaaattcgct atcaacctga agaaagttga agagcgtgaa
    19201 ctgccggaac tgactgcaga attcatcaaa cgtttcggcg ttgaagatgg ttccgtagaa
    19261 ggtctgcgcg ctgaagtgcg taaaaacatg gagcgcgagc tgaagagcgc catccgtaac
    19321 cgcgttaagt ctcaggcgat cgaaggtctg gtaaaagcta acgacatcga tgtaccggct
    19381 gcgcttattg acagcgaaat cgacgttctg cgtcgccagg ctgcacagcg tttcggtggc
    19441 aacgaaaaac aagctctgga actgccgcgc gaactgttcg aagaacaggc taaacgccgc
    19501 gtagttgttg gcctgctgct gggcgaagtt atccgcacca acgagctgaa agctgacgaa
    19561 gagcgcgtga aaggcctgat cgaagagatg gcttcagcgt acgaagatcc gaaagaagtt
    19621 atcgagttct acagcaaaaa caaagaactg atggacaaca tgcgcaatgt tgctctggaa
    19681 gaacaggctg ttgaagctgt actggcgaaa gcgaaagtga ctgaaaaaga aaccactttc
    19741 aacgagctga tgaaccagca ggcgtaattt acgcagcata acgcgctaaa ttcgcacaaa
    19801 ggcccgtcac cgccaggtgg tgggcttttt ttgtcatgaa ttttgcatgg aaccgtgcga
    19861 aaagcctctt tcggtgttag cgtaacaaca aaagattgtt atgcttgaaa tatggtgatg
    19921 ccgtacccat aacacaggga ctagctgata atccgtccat aaggttacaa tcggtacagc
    19981 aggttttttc aattttatcc aggagacgga aatgtcatac agcggcgaac gagataactt
    20041 tgcaccccat atggcgctgg tgccgatggt cattgaacag acctcacgtg gtgagcgctc
    20101 ttttgatatc tattctcgtc tacttaagga acgcgtcatt tttctgactg gccaggttga
    20161 agaccatatg gctaacctga ttgtggctca gatgctgttc ctggaagcag aaaacccaga
    20221 aaaagatatc tatctgtaca ttaactcccc aggcggggtg atcactgccg ggatgtctat
    20281 ctatgacacc atgcagttta tcaagcctga tgtcagcacc atctgtatgg gccaggcggc
    20341 ctcgatgggc gctttcttgc tgaccgcagg ggcaaaaggt aaacgttttt gcctgccgaa
    20401 ttcgcgcgtg atgattcacc aaccgttggg cggctaccag ggccaggcga ccgatatcga
    20461 aattcatgcc cgtgaaattc tgaaagttaa agggcgtatg aatgaactta tggcgcttca
    20521 tacgggtcaa tcattagaac agattgaacg tgataccgag cgcgatcgct tcctttccgc
    20581 ccctgaagcg gtggaatacg gtctggtcga ttcgattctg acccatcgta attgatgcca
    20641 gaggcgcaac tgtgccgcta tacttatcca gggcggcaca acgctgtaag cggcttgcgc
    20701 ctgagaatgg catttgcgtc gtcgtgtgcg gcacaaagaa caaagaagag gttttgaccc
    20761 atgacagata aacgcaaaga tggctcaggc aaattgctgt attgctcttt ttgcggcaaa
    20821 agccagcatg aagtgcgcaa gctgattgcc ggtccatccg tgtatatctg cgacgaatgt
    20881 gttgatttat gtaacgacat cattcgcgaa gagattaaag aagttgcacc gcatcgtgaa
    20941 cgcagtgcgc taccgacgcc gcatgaaatt cgtaaccacc tggacgatta cgttatcggc
    21001 caggaacagg cgaaaaaagt gctggcggtc gcggtataca accactacaa acgtctgcgc
    21061 aacggcgata ccagcaatgg cgtcgagttg ggcaaaagta acattctgct gatcggtccg
    21121 accggttccg gtaaaacgct gctggctgaa acgctggcgc gcctgctgga cgtcccgttc
    21181 accatggccg acgcaaccac gctgaccgaa gccggttatg tgggcgaaga cgttgaaaac
    21241 atcattcaga agctgttgca aaagtgcgat tacgacgtac agaaagcgca gcgcgggatt
    21301 gtctacatcg atgaaatcga caagatttct cgtaagtcag acaacccgtc tattacccgt
    21361 gacgtttccg gtgaaggcgt acagcaggca ctgttgaaac tgatcgaagg tacggtagct
    21421 gctgttccac cgcaaggtgg acgtaaacat ccgcagcagg aattcttgca ggttgatacc
    21481 tctaagatcc tgtttatttg tggcggtgcg tttgccggtc tggataaagt gatttcccat
    21541 cgtgtagaaa ccggctccgg cattggtttt ggcgcgacgg taaaagcgaa gtccgacaaa
    21601 gcaagcgaag gcgagctgct ggcgcaggtt gaaccggaag atctgatcaa gtttggtctt
    21661 atccctgagt ttattggtcg tctgccggtt gtcgcaacgt tgaatgaact gagcgaagaa
    21721 gctctgattc agatcctcaa agagccgaaa aacgccctga ccaagcagta tcaggcgctg
    21781 tttaatctgg aaggcgtgga tctggaattc cgtgacgagg cgctggatgc tatcgctaag
    21841 aaagcgatgg cgcgtaaaac cggtgcccgt ggcctgcgtt ccatcgtaga agccgcactg
    21901 ctcgatacca tgtacgatct gccgtccatg gaagatgtcg aaaaagtggt tatcgacgag
    21961 tcggtaattg atggtcaaag caaaccgttg ctgatttatg gcaagccgga agcgcaacag
    22021 gcatctggtg aataattaac cattcccata caattagtta accaaaaagg ggggatttta
    22081 tctccccttt aatttttcct ctattctcgg cgttgaatgt gggggaaaca tccccatata
    22141 ctgacgtaca tgttaataga tggcgtgaag cacagtcgtg tcatctgatt acctggcgga
    22201 aattaaacta agagagagct ctatgaatcc tgagcgttct gaacgcattg aaatccccgt
    22261 attgccgctg cgcgatgtgg tggtttatcc gcacatggtc atccccttat ttgtcgggcg
    22321 ggaaaaatct atccgttgtc tggaagcggc gatggaccat gataaaaaaa ttatgctggt
    22381 cgcgcagaaa gaagcttcaa cggatgagcc gggtgtaaac gatcttttca ccgtcgggac
    22441 cgtggcctct atattgcaga tgctgaaact gcctgacggc accgtcaaag tgctggtcga
    22501 ggggttacag cgcgcgcgta tttctgcgct ctctgacaat ggcgaacact tttctgcgaa
    22561 ggcggagtat ctggagtcgc cgaccattga tgagcgagaa caggaagtgc tggtgcgtac
    22621 tgcaatcagc cagttcgaag gctacatcaa gctgaacaaa aaaatcccac cagaagtgct
    22681 gacgtcgttg aatagcatcg acgatccggc gcgtctggcg gataccattg ctgcacatat
    22741 gccgctgaaa ctggctgaca aacagtccgt tctggagatg tccgacgtta acgaacgtct
    22801 ggaatatctg atggcaatga tggaatcgga aatcgatctg ctgcaggttg agaaacgcat
    22861 tcgcaaccgc gttaaaaagc agatggagaa atcccagcgt gagtactatc tgaacgagca
    22921 aatgaaagct attcagaaag aactcggtga gatggacgac gcgccggacg aaaacgaagc
    22981 cctgaagcgc aaaatcgacg cggcgaagat gccgaaagag gcaaaagaga aagcggaagc
    23041 agagttgcag aagctgaaaa tgatgtctcc gatgtcggca gaagcgaccg tagtgcgtgg
    23101 ttatatcgac tggatggtac aggtaccgtg gaatgcgcgc agcaaggtca aaaaagacct
    23161 gcgtcaggcg caggaaatcc ttaataccga ccattatggt ctggagcgcg tgaaagatcg
    23221 catccttgag tatcttgcgg ttcaaagccg tgtcaacaaa atcaagggac cgatcctttg
    23281 cctggtaggg ccgccggggg taggtaaaac ctccctgggt cagtccattg ccaaagccac
    23341 cgggcgtaaa tatgtccgta tggcgctggg cggcgtgcgt gatgaagcgg aaatccgtgg
    23401 tcaccgccgt acttacatcg gttctatgcc gggtaaattg atccagaaaa tggcgaaagt
    23461 gggcgttaaa aacccgctgt tcctgctcga tgagatcgac aaaatgtctt ctgacatgcg
    23521 tggcgatccg gcttccgcac tgcttgaagt gctggatcca gagcagaacg tggccttcag
    23581 cgatcactac ctggaagtgg attacgatct cagcgacgtg atgtttgtcg cgacgtcgaa
    23641 ctccatgaac attccggcac cactgctgga tcgtatggaa gtgattcgcc tctccggtta
    23701 taccgaagat gaaaaactga acatcgccaa acgtcacctg ctgccgaagc agattgaacg
    23761 taatgcactg aaaaaaggtg agctgaccgt cgacgatagc gccattatcg gcattattcg
    23821 ttactacacc cgtgaggcgg gcgtgcgtgg tctggagcgt gaaatctcca agctgtgccg
    23881 taaagcggtt aagcagttac tgctcgataa gtcattaaaa catatcgaaa ttaacggcga
    23941 caacctgcat gactaccttg gtgttcagcg tttcgactat ggtcgcgctg ataacgaaaa
    24001 ccgtgtcggt caggtaaccg gtctggcgtg gacggaagtg ggcggtgact tgctgaccat
    24061 tgaaaccgca tgtgttccgg gtaaaggcaa actgacctat accggttcgc tcggcgaagt
    24121 gatgcaggag tctattcagg ctgcgttaac ggtggttcgc gcgcgtgcgg aaaaactggg
    24181 gatcaaccct gatttttacg aaaaacgtga catccacgtc cacgtaccgg aaggtgcgac
    24241 gccgaaagat ggtccgagtg ccggtattgc tatgtgcacc gcgctggttt cttgcctgac
    24301 cggtaacccg gttcgtgccg atgtggcaat gaccggtgag atcactctgc gtggtcaggt
    24361 actgccgatc ggtggtttga aagaaaaact actggcagcg catcgcggcg ggattaaaac
    24421 agtgttaatt ccgttcgaaa ataaacgcga tctggaagag attcctgaca acgtaattgc
    24481 cgatctggac attcatcctg tgaagcgcat tgaggaagtt ctgactctgg cgctgcaaaa
    24541 tgaaccgtct ggcatgcagg ttgtgactgc aaaatagtga cctcgcgcaa aatgcactaa
    24601 taaaaacagg gctggcaggc taattcgggc ttgccagcct ttttttgtct cgctaagtta
    24661 gatggcggat cgggcttgcc cttattaagg ggtgttgtaa ggggatggct ggcctgatat
    24721 aactgctgcg cgttcgtacc ttgaaggatt caagtgcgat ataaattata aagaggaaga
    24781 gaagagtgaa taaatctcaa ttgatcgaca agattgctgc aggggctgat atctctaaag
    24841 ctgcggctgg ccgtgcgtta gatgctatta ttgcttccgt aactgaatct ctgaaagaag
    24901 gggatgatgt agcactggta ggttttggta cttttgccgt taaagagcgt gctgcccgta
    24961 ctggccgcaa cccgcagacc ggtaaagaga tcaccatcgc tgccgctaaa gtaccgagct
    25021 tccgtgcagg taaagcactg aaagacgcgg taaactaagc gttgtcccca gtggggatgt
    25081 gacgaagttc aagggcgcat ctactgatgt gcctttttta tttgtattcg gtgactttct
    25141 gcgtcttgtg ggctgacaat tgcccccgtt tcttgtcaca ataggccttt gcgcgcatcg
    25201 atacgttgcg tgaggtacac agtcatctac agcggagtgt tgttacacca tgatggacag
    25261 cttacgcacg gctgcaaaca gtctcgtgct caagattatt ttcggtatca ttatcgtgtc
    25321 gttcatattg accggcgtga gtggttacct gattggcgga ggcaataact acgccgcaaa
    25381 agtgaatgac caggaaatca gccgtgggca attcgagaat gccttcaaca gcgagcgtaa
    25441 tcgcatgcag caacagctgg gcgatcaata ctccgagctg gcagcgaacg aaggctatat
    25501 gaaaaccctg cgtcaacagg tgctgaatcg tctgatcgac gaggcgctgc tggatcagta
    25561 cgcacgtgag ctgaaactgg gtatcagcga tgagcaggtt aaacaggcga ttttcgcgac
    25621 cccagccttc caggttgatg gcaaatttga taacagccgc tataacggta tcctcaacca
    25681 gatggggatg accgccgatc agtacgccca ggcgctgcgt aaccagctca ctacccaaca
    25741 gctgattaac ggcgttgctg gtaccgattt tatgctgaaa ggtgaaaccg acgagctggc
    25801 ggcactggtc gctcaacaac gcgtggtgcg tgaagcgact atcgatgtta acgcgctggc
    25861 ggcgaagcag cctgtgaccg aacaggaaat tgccagctac tacgaacaaa acaaaaacaa
    25921 tttcatgacg ccggaacaat tccgcgtgag ttacatcaag ctggatgccg caacgatgca
    25981 gcaaccggtt agcgatgcgg atctccagag ctactacgat cagcatcagg atcaattcac
    26041 ccagccgcag cgtacccgtt acagcatcat ccagaccaaa actgaagatg aagcgaaagc
    26101 ggtacttgat gagctgaata aaggcggtga ttttgctgca ttagccaaag aaaaatctgc
    26161 cgatattatc tctgctcgta acggcggcga tatgggttgg ttagaagatg ccactatccc
    26221 ggatgaactg aaaaatgctg gtctgaaaga aaaaggccaa ctgtctggtg tcatcaaatc
    26281 ttcggtcggt ttcctgattg tacgtctgga cgacattcag ccagcgaaag tgaaatcgtt
    26341 agacgaagta cgtgacgatg tcgcggcgaa agtgaaacac gaaaaagccc tcgatgcgta
    26401 ctacgcgctg cagcagaaag tgagcgatgc ggcaagcaac gacaccgagt ctctggccgg
    26461 tgcagagcaa gctgccggcg ttaaagccac tcagacgggt tggttcagca aagagaacct
    26521 gccggaagag ttgaacttca agccggttgc tgacgctatc tttaacggcg gtctggtagg
    26581 tgaaaacggc gcgccgggca tcaactctga catcatcacc gtagacggcg accgcgcatt
    26641 cgtgctgcgc atcagcgagc acaaaccgga agcggtgaaa ccgttggcag atgttcagga
    26701 acaagttaag gcactggttc agcacaacaa agctgaacaa caggcgaaag tggatgctga
    26761 gaaactgctg gttgatttga aagccggcaa aggtgcggaa gctatgcagg ctgccggtct
    26821 gaaatttggc gagccgaaaa ccttaagccg ttccggtcgt gacccgatta gccaggcggc
    26881 gtttgcactg ccactgccag cgaaagacaa accgagctac ggtatggcga ccgatatgca
    26941 aggtaatgtg gttctgctgg cgctggatga agtgaaacaa ggttcaatgc cggaagatca
    27001 gaaaaaagcg atggtgcagg gtatcaccca gaacaacgca caaatcgtct ttgaagctct
    27061 gatgagtaac ctgcgtaaag aggcgaaaat caaaattggc gatgcgttgg aacagcaata
    27121 atcctgaagc cgcctcgcaa aaaaatgctt tgcagctgta acaagaaaag gtcgctttcg
    27181 cggccttttc catttctgaa cattgccatt tgtttactgt tttcactgcc gttaaggtga
    27241 ttccactgtt aacaaacaag gagaaaacag tatgaaacac ggaattaaag cactgctcat
    27301 taccctgtcc ctggcctgtg ccggaatgtc tcatagcgcg ctggcggcag cttctgtggc
    27361 gaaaccgacg gcggtagaaa ccaaagcgga agctcctgca gcacaaaata aagcagcagt
    27421 accggcgaaa gccagtgacg aagaaggctc ccgggtcagc attaataatg ccagcgcgga
    27481 agagctagcc cgcgcgatga atggcgttgg cctgaagaaa gcgcaagcga ttgtcagtta
    27541 tcgcgaagag tacggtccgt ttaaaacggt ggaggatcta aagcaggtgc cggggatggg
    27601 caattcgctg gtggaacgta atctggcggt attaaccctg taattaattt gcatagtggc
    27661 aattttttgc cagactgaag aggtcatacc agttatgacc tctgtactta taacagtttt
    27721 tataaaaaca acgtaaggtt attgcgctat gcaaacacaa atcaaagttc gtggatatca
    27781 tctcgacgtt taccagcacg taaacaacgc ccgctacctt gaatttctcg aagaagcccg
    27841 ctgggatggg ttggaaaata gcgacagttt tcactggatg acggcccata acatcgcttt
    27901 cgtggtggtg aatatcaata ttaactatcg tcgtccggcg gtattaagcg acttgttaac
    27961 cattacgagc cagttacagc aattaaacgg taaaagcggc atcttaagcc aggtcattac
    28021 actggagccg gaagggcagg tggtagcgga tgcgcttatt acgtttgttt gtattgatct
    28081 taaaacgcag aaagcattag ctctggaagg ggaattgcgc gaaaagctgg agcagatggt
    28141 taagtaaacg ttttgtggtg ccggatgctc aagccgcatc cggcgacacc cggaataatt
    28201 acttcaaccc ggttttctgc ttcatcgctg ccatcaccgt cggtttatcg gccagataat
    28261 gattcaaacc gttggcgcgt aaattacatg ccgcacaatg accgcaaccg tcgcctttaa
    28321 tgccgttata gcaggtcaac gtttcgttac ggactaaatc cagtttgccg taataatctg
    28381 ccagcgccca ggtttccgct ttatcaatcc acatcagcgg cgtttcaaaa cgaatatctt
    28441 tcgccatgcc caaactgacg gcatggttta gtactttcac aaactcatcg cggcaatccg
    28501 ggtagccgga gaaatccgtt tcgcagacgc cggtaattac ggcttctgct tttacctgat
    28561 acgcatatat tgccgccagc gtcaggaaca aaatattacg ccctgggaca aacgtattcg
    28621 ggatgccatc ggcttcaggt tcataatcag gcaccggaat gctgtcacgc gtcaggctgc
    28681 tgaccgccag ctcgttgagc agcgttacat caagaacctt atgcgcgcgt gcccccagtt
    28741 tcagcgccag ttcgcgtgcc acgtcgattt ctgcgcgatg gcgctgaccg taatcgaacg
    28801 tcacgcaatg gacttcatca tattgttgta atgcctgcac cagacaggtg gtggaatcct
    28861 ggcctccact gaacacaacg acagcacgtt tcatagataa tcctgcttga acaataaaag
    28921 cgttatggta acgcctgcga ttaacccgga ccagctattc attgcgacgg cgcgggaagc
    28981 caggcttcgg taaattcaaa ccagccgcgc ggtgtcagtc gcacaccgtt cacgccggga
    29041 ggggcactaa tgcgatagtg atagttgaac agcggcgtca gcgtcgcatc tgtcattaac
    29101 tggctaaaaa ccgctttcag ggcattaaat cggttttcct catcaggcat tacttgcacc
    29161 gcgtccagtg tcgattgtag atgtgcgtat gctggagcgt cgaaaacatg tggccacagc
    29221 ggatcgcagc gcagccattg ctccagagta tattccggtg cttcgccaat taatctgtcg
    29281 cccatcatga ggtctgcgtg tgccagtagg gtcgtgtcgt cccagttttt tgcgttatga
    29341 aaaataattg tgagttcaca gccttccgct gccagtgtcg cctgtaggcg ttctgccatg
    29401 gtatgaagtt ctatcgggag gtgataaacc agcgtcaagg ttttcggtag tttgacttca
    29461 tccggtactt gccaatgcgg aatagtccag cctggcagta atgcatgact ggcggtgatc
    29521 aggttttctc cgacttctaa cgtttgtaat aaaccggatt gatgaataat ggagatcact
    29581 tttcgcgcct gccagaggga gagtcgggga cttttgcgca acgttaaata gcaaaaacct
    29641 aaactgatgc cactactgac ctggctgacc cgttgcagct cctccggttt gccgatggtg
    29701 atttgcacgg gatgccgaca actggttccc aaatcttttt cgaaaagcgg cggagttatc
    29761 cagtactcaa ccgctttaag cagcggatga cgtaaatggt aataatcatg gctttccagg
    29821 cgcaccagtt cagctgtgaa ttgtgttaag cgaaaaggac ccgtgccaat cagtgggaat
    29881 tgcggatgcg ccagatggct gcaatagctc gccagccggt gcgcaagcca gtaatcgggg
    29941 cgatgtaaaa agaaggtcag acactgcgga tgggtgacct caatacgctt cacgctaata
    30001 aataattgat ccagtgctgg cagttgtaac agcatcaata atcgctggtg taagtgtgag
    30061 gcttttactg catcgccgtt atgccagtgt agggttgaac gaagataaaa gtcccagcgt
    30121 aacccgtcag cagaggtttc ccagtgatgc gctaaatcgc caatcgggcg ctgggtatta
    30181 ttatcgaagc gggtcaggcc ggaaaatatc tgtccggcga gatgctgctc ggcacggccg
    30241 ggcaaaaagc ccggttgtag cggttcgagc gggcgatagt agggaatacg caacgtgggt
    30301 gtatcgtttt gccattgtcc gcccataaac ggctgtaaca gagtgcgcag ctcacctggg
    30361 gccagttgcg ccagctccag cacatcttgc tgctttccgg tttccagtgc cttttccatc
    30421 atcgcattgc gtagcgattc tggcgtgacc agaaagcgta attgtccgcg ctttccgcgt
    30481 cctgactgcg cctgccactc cagccatccc gcttcctgtg cctgacgcaa cagcgtacga
    30541 acatggcgtt cgctgcaaaa acagcgttcg gccagttcgc tgacggtgac ggtttgcggc
    30601 tttccggcgg aaggttgcca cagacgttga tactggttaa ggcggttgag cagtcgcata
    30661 aaaacccgga acaatattat ttaactattc actattactt ccgtatatat caggtgatac
    30721 tcaatcacca ttaaccgtgt cacagagtgg agaaagaaat ggctcgtctg gcagcatttg
    30781 atatggatgg cactttattg atgcctgacc atcatttagg tgagaaaacc ctctctactt
    30841 tggcgcgact gcgtgaacgc gacattaccc tcacttttgc cacggggcgt catgcgctgg
    30901 agatgcagca tattctcggg gcgatatcgc tggatgcgta tttgattacc ggcaacggaa
    30961 cgcgcgtgca ttctctggaa ggtgaacttt tacatcgtga tgatttacct gcggatgtcg
    31021 cggagctggt gctgtatcag caatgggata cccgagccag catgcatatt ttcaatgacg
    31081 acggttggtt taccgggaaa gagatccctg cgttgttgca ggcatttgtc tatagcggtt
    31141 ttcgttatca aataatcgat gtcaaaaaaa tgccactcgg cagcgtcacc aagatctgct
    31201 tctgtggcga tcacgacgat cttacacgtt tgcagatcca gctacacgaa gcattaggcg
    31261 agcgtgcaca tttgtgtttt tccgccacgg attgccttga agtgctgccg gtgggctgca
    31321 ataaaggcgc tgcactgacg gtgctgaccc aacatttagg tttatcgttg cgcgattgca
    31381 tggcctttgg tgatgcgatg aacgatcgcg aaatgttagg cagcgtcggt agcggattta
    31441 ttatgggcaa tgcgatgccg caactgcgcg cggagctccc gcatttaccg gtgattggac
    31501 attgccgaaa tcaggctgtc tctcactatt tgacgcactg gctggactat ccacatctac
    31561 cttattcccc cgaataacga gatcccttcc agcaccgggc aattgcccgg tttttttgcg
    31621 ttgaatttgt cattttgtgc cgtggtgttt aaaccgcaca gaataaattg tcgcgatttc
    31681 acctttaaaa tagaattaaa agagaaaaaa attctccgtg gaagggctat gttagataaa
    31741 attgaccgta agctgctggc cttactacag caggattgca ccctctcttt gcaggcactg
    31801 gctgaagccg ttaatctgac aaccaccccc tgctggaagc gcctgaaacg gctggaggac
    31861 gacggtatcc ttatcggcaa agtcgccctg ctggatccgg aaaaaatagg cctcggcctg
    31921 accgcctttg tgctgataaa aacgcaacat cacagcagcg aatggtattg ccgctttgtc
    31981 acggtggtta ccgaaatgcc agaagtgctg gggttctggc gcatggctgg tgaatacgat
    32041 tatctgatgc gcgtccaggt tgccgacatg aaacgctacg acgagtttta taagcgtctg
    32101 gtaaacagcg tgccggggct gtcggacgtc acttccagct tcgcgatgga acagattaaa
    32161 tacaccactt ctttacccat cgaataaata tccagaatca ggtcaggaca caacgcgtgc
    32221 gattatttgc tcaattaagc tggtatttcc gtcgggaatg gcgtcgctat ctcggggctg
    32281 tcgccttgct tgtcattatc gcgatgctgc aactggttcc accaaaagtg gttggtattg
    32341 ttgtcgatgg cgtgacagaa caacacttta ctaccgggca gatcctgatg tggatcgcca
    32401 ccatggtgct gattgccgtt gtggtttatc tcctgcgtta cgtctggcgc gtattgctgt
    32461 ttggtgcgtc ttatcaactg gctgttgaac tgcgtgaaga ttattaccgt cagctaagcc
    32521 ggcagcatcc tgagttttac ctgcgtcatc gcacgggtga cctcatggct cgtgcgacta
    32581 atgacgtcga tcgcgtcgtg tttgccgccg gagaaggggt gctgacgctg gtggattcac
    32641 tggtaatggg ctgcgctgtg ttgattatga tgtctacgca aattagctgg cagttgacct
    32701 tatttgccct gttaccgatg ccagtgatgg cgatcatgat taagcgcaac ggcgatgctt
    32761 tgcatgaacg ctttaagctg gcacaggcgg cgttttccag tcttaatgac cgcacccagg
    32821 aaagcctcac cagtatccgc atgatcaaag cctttggtct ggaagatcgc cagtcggcgt
    32881 tatttgccgc ggatgccgaa gataccggca aaaaaaacat gcgggtggcg cgtattgatg
    32941 ctcgtttcga cccgaccatc tatatcgcga ttggtatggc gaacttgctg gcgattggcg
    33001 gcggtagttg gatggtggtg cagggcagtt taacgctggg ccagctcacc agttttatga
    33061 tgtatttagg tctgatgatt tggccaatgc tggcgctggc atggatgttt aacattgtgg
    33121 aacgtggtag tgctgcgtac agccgtattc gcgcgatgct ggcggaagcg ccggtggtga
    33181 acgatggtag cgaacccgtg ccggaagggc gtggcgaact ggatgtaaat attcaccagt
    33241 tcacgtatcc gcagactgac catcctgcgc tggaaaacgt caatttcgcc ctgaaacccg
    33301 gtcagatgct gggtatctgc gggccgactg gttccggcaa aagtaccctg ttgtcgctca
    33361 ttcagcgtca tttcgacgtc agcgaggggg atattcgctt tcatgatatt cctctgacga
    33421 agttacaact cgatagctgg cgtagccgcc tggcggtagt tagccagacg ccattccttt
    33481 tttctgacac cgtggcgaat aacatcgcgc tgggttgccc gaatgccacc cagcaagaga
    33541 ttgagcatgt cgcgcggtta gccagcgtac atgacgatat tttgcgtcta ccgcaaggtt
    33601 acgatacaga ggtgggcgag cgcggtgtga tgctttccgg cgggcaaaaa cagcgtatct
    33661 ccattgctcg tgcgttatta gtcaacgcgg aaatcctcat ccttgatgat gcgctttcgg
    33721 cggtggacgg acgcactgag caccagatcc tgcataacct gcgtcagtgg gggcagggaa
    33781 gaacggtaat catcagtgcc catcgccttt ctgcactgac ggaagccagt gaaattattg
    33841 tgatgcagca cggacatatc gcccagcgtg gcaatcatga tgtgctggca caacaaagcg
    33901 gctggtatcg cgatatgtat cgctatcaac aactggaggc ggcgctcgac gacgctccgg
    33961 aaaatcgcga ggaggccgtc gatgcgtagt tttagccaac tgtggccgac tctcaagcgc
    34021 ctgttagcgt acggttcacc gtggcgtaaa ccgctgggga ttgcggtcct gatgatgtgg
    34081 gttgcggcgg cggcagaagt cagtgggccg ctgcttatca gctattttat cgacaatatg
    34141 gtagcgaaaa ataacctgcc gttgaaagtg gttgcagggc tggctgcggc gtatgttggg
    34201 ctgcaactgt ttgccgccgg gctacattac gcgcagtcgc tgctgtttaa tcgggcggca
    34261 gtaggcgtag tgcaacagtt gcgtaccgac gtgatggatg ctgcgttacg ccagccatta
    34321 agcgagtttg atacccaacc cgtcgggcag gtgatttccc gcgtcactaa tgacactgaa
    34381 gtgatccgcg atctctacgt taccgtagtg gcaactgtcc tgcgcagtgc cgcactggtg
    34441 ggcgcgatgc tggtggcgat gttcagcctc gactggcgaa tggcgctggt ggcgataatg
    34501 attttcccgg tggtgctggt ggtaatggtg atataccagc gttacagcac gccaattgtc
    34561 cgtcgtgtgc gcgcctatct ggcggatatc aacgacggct ttaacgaaat catcaatggc
    34621 atgagcgtta tccagcagtt tcgtcagcag gcgcgatttg gcgaacgtat gggtgaggcc
    34681 agtcgttcac actatatggc gaggatgcaa accctgcgcc tcgacggttt tctgctgcgt
    34741 ccgctgctga gtctgttttc atcgctcatt ctctgtggct tgttgatgct gtttggcttc
    34801 tccgccagcg gcaccattga agtgggcgtg ttgtatgcgt ttatcagcta tcttgggcga
    34861 cttaacgaac ccttaatcga actgaccacg cagcaggcga tgctgcaaca ggctgttgtt
    34921 gctggtgagc gcgtgtttga gctgatggac ggaccgcgcc agcaatatgg caatgatgat
    34981 cgcccgttac agagtggcac catcgaagtc gataacgtgt catttgctta tcgcgatgac
    35041 aatctggtgc taaagaacat taatctctct gtgccttcgc gtaactttgt ggcgctggtc
    35101 gggcataccg gcagtggtaa aagtaccctc gccagtttat tgatgggcta ttacccgcta
    35161 acggaaggtg agattcgcct tgatggtcgt ccattaagtt cgctaagtca cagcgcgctg
    35221 cgccagggcg tggcaatggt gcagcaagat ccggtggtgc tggcggatac cttcctcgcc
    35281 aacgtgacgc tggggcggga tatctccgaa gaacgcgtct ggcaggcgct ggaaaccgtg
    35341 caactggcgg agctggcacg cagtatgagc gacgggattt atacgccgct gggcgagcag
    35401 gggaataatc tctctgtcgg gcaaaagcaa ctgctggcac tggcgcgcgt gctggtcgag
    35461 acgccgcaaa tcctgatcct tgatgaggca accgccagca ttgactccgg tactgaacag
    35521 gcgattcaac atgctctggc ggcggtgcgt gaacatacca cgctggtagt gattgctcac
    35581 cgcttatcga ctattgttga tgccgacact attctggtgc ttcatcgtgg gcaagccgtg
    35641 gagcagggca ctcaccagca actgctggcg gcccagggac gctactggca gatgtatcaa
    35701 ctgcaacttg cgggcgaaga gcttgcagcc agcgtgcgtg aagaagaatc attgagcgcc
    35761 tgaatagcgc aatatctcat cgttggtgca aaaatgtaac gcactgtgca ctgtcatagt
    35821 gcgttttcat tttcaatctt cttaacttcc tgctctcttt ctcgtttttc atttctggca
    35881 caccgcttgc aataccttct tcgtgtagca gaaccattac cgaattctga ccggagggga
    35941 tctatgaagc tggtgaccgt gataatcaaa ccattcaagc tggaagacgt tcgtgaagcg
    36001 ttatcttcca ttggtattca gggcctgacc gtcaccgaag tgaaaggttt cgggcgtcag
    36061 aaagggcatg ccgagctgta ccggggggcg gaatacagcg tcaacttcct gccaaaagta
    36121 aaaattgatg tggcgattgc tgatgaccaa ctcgatgaag tgatcgatat cgtcagtaag
    36181 gcggcttaca ccggaaaaat tggcgacggc aaaatcttcg tcgctgaatt gcaacgcgtc
    36241 attcgtattc gtaccggcga agccgacgaa gcggcgctgt aatctctggc acacagcaac
    36301 aggaacgaaa aatgaagata gcgacgataa aaactgggct tgcttcactg gcgatgctgc
    36361 cgggactggt aatggctgca cctgcggtgg ccgataaagc cgacaatgcg tttatgatga
    36421 tttgtactgc actggtgctg tttatgacta ttccggggat tgccctgttt tacggtgggt
    36481 tgattcgcgg caaaaacgtg ctgtcgatgc tgacgcaggt gacggtgacg tttgcactgg
    36541 tctgtattct ctgggtggtt tacggttact cgctggcgtt tggtgagggc aacaacttct
    36601 tcggcaacat taactggttg atgctgaaaa acatcgaact gacggcggtg atgggcagca
    36661 tttatcagta tatccacgta gcgtttcagg gatcgtttgc ctgcattacc gtaggcttga
    36721 tagttggggc gctggcggaa cgaatccgct tctcagctgt gttgattttc gtggtggtat
    36781 ggctgacgct ctcttacatt cctattgcgc atatggtgtg gggcggtggt ttgctggctt
    36841 ctcacggtgc gctggatttc gcgggtggca cggtggtgca tattaacgcc gcaatcgccg
    36901 gtctggtggg cgcgtatctg ataggcaaac gcgtgggctt cggtaaagag gcgtttaaac
    36961 cgcacaacct gccgatggtc ttcaccggga ctgccattct ctatatcggt tggtttggct
    37021 ttaacgccgg gtcagcgggc acggcgaatg aaatcgcggc actggcattt gtgaatactg
    37081 tggtcgcaac ggcggcggca attcttggct ggatcttcgg tgaatgggcg ctgcgtggta
    37141 agccttcact gctgggagcg tgttctggcg cgattgccgg tctggtcggc gtgacgccag
    37201 cgtgtggcta cattggtgtt ggcggcgcgt tgattatcgg cgtggtagct ggtctggcgg
    37261 gcttgtgggg cgttaccatg ctcaaacgct tgctgcgggt ggatgatccc tgcgatgtct
    37321 tcggtgtgca cggcgtttgt ggcattgtcg gctgtatcat gactgggatt tttgccgcca
    37381 gctcgctggg cggcgtgggc ttcgctgaag gtgtgacgat gggccatcag ttgctggtac
    37441 agttggaaag cattgccatt acgattgtgt ggtctggtgt ggtggcattt atcggctaca
    37501 aactggcgga tctgacggtt ggtctgcgtg tgccggaaga acaagagcga gaagggctgg
    37561 acgtcaacag tcacggcgag aatgcctata acgcataaca agcactgcaa aaaacagccg
    37621 gacggttttc acctccggct atttttttaa ttgtgattac gcatcacccc ttcctgaacg
    37681 gtcgaggcaa ccagtacgcc gtcctgggta taaaactcac cgcgcacaaa gccacgtgcg
    37741 ctggacgccg aggtgctctc cacgctatac agcagccatt cattcaaatt aaacgggcga
    37801 tggaaccaca tggaatggtc aatggtggca atctgaatcc ccggttcgag aaaaccgata
    37861 ccgtgcggct gtagagctac cggcaggaag ttaagatcag aagcgtaacc gagcagatac
    37921 tgatgaacgc gcaggtcatc cggcacgcta ccatttgcgc ggatccacac ctgacgatgt
    37981 ggttctgcga cgtgaccttt cagtgggtta tgaaactcca ccggacggac ttccagcgga
    38041 cgatcgcaga tgaatttatc tttaagcact ggcggcagca ggtgcgccag cgattgggcg
    38101 atttgcgttt ccgaaggcag gccatcaggc gctggcgcgg acggcattgt tttttgatgt
    38161 tcgaaacccg cttctggtgc ctggaaagag gccgtcatat aaaaaatcgg tttgccgttt
    38221 tgaatagcag caacccggcg ggcgctgaag ctgttaccgt cacgcagcgt ttcgacatca
    38281 taaataatcg gcttcttact atcgccaggg cgaagaaagt agctgtgaaa cgaatgtacc
    38341 agccgctctt cagggacggt ctcttttgca gcatacaagg cctgacccac gacctggccg
    38401 ccaaatacct ggcgtaaacc taaatcttca ctctggccgc gaaagagtcc ttcctcaatt
    38461 ttttccagat ttaacaatgt cagtaaattt tttagcgcct gactcatata actctccagt
    38521 aacaaagctg ccgcagcaag ccaaagtgag ttgagtataa cgcaaatttg ctactggtcc
    38581 gatgggtgca atggtctgaa ttacgggcta attacaggca gaaatgcgtg atgtgtgcca
    38641 caattgttga cgttactatt ttgttaacca ctcttccggc gaggaaagtt agcccgctgg
    38701 tgcattgata ataaggagaa atgaatgaaa ctcgtgcaca tggccagtgg tttagcggtt
    38761 gcgattgcgt tggcggcttg cgcagataaa agcgcggata ttcagacgcc agccccggct
    38821 gcaaatacgt ctatttcagc aacacaacaa cctgctatcc agcaaccgaa tgtctccggt
    38881 accgtctgga tccgtcagaa agtcgcactg ccgcctgatg ctgtgctgac cgtgacactt
    38941 tctgacgcgt cgttagccga tgcaccgtca aaagtgctgg cgcagaaagc ggtgcgtact
    39001 gaaggtaaac agtcaccatt cagctttgtt ctgccattta acccggcaga tgttcagccg
    39061 aacgcgcgta ttctgttgag tgcggcgatt accgtgaatg acaaactggt atttattacc
    39121 gataccgttc agccggtgat caaccagggg ggaaccaaag ccgacctgac attggttccg
    39181 gttcagcaaa ccgccgtgcc tgtccaggcc agtggtggcg caacaactac cgtaccatcg
    39241 acttcaccga cccaggtgaa tccatcttcg gcagttcctg cccctacgca atactaagcg
    39301 ttactaaccc ctttccatct ggagaggggt tagtagttcc agcgatagcg ctgtaagtcg
    39361 atttgcccgc ttcccgatac catcacacct tctgccagta atgcctgtcg ttgacgctgt
    39421 aaatccggtc cggttagcga aatggtgccg tggcgattaa ccacccggtg ccagggcaag
    39481 gtgctgcctt cggggagacg ctttaacaca ccgccaacct ggcgcgcggc gcggggcgat
    39541 cccgccagtt tcgccacatc accgtaagtg gtgacatacc cttcgggaat agcggctacg
    39601 atttgccaga cgcgttgggg aaatgaatct tctttttcca tcttttcttc ctgagataat
    39661 ttttcagcat aatctggaaa aacgcccgag tgaagccgca ttgcgcaaga aaccagcatc
    39721 tggcacgcaa tgggttgcaa ttagccgggg cagcagtgat aatgcgcctg cgcgttggtt
    39781 ctcaacgctc tcaatggggg ctctgttggt tctcccgcaa cgctactctg tttaccaggt
    39841 caggtccgga aggaagcagc caaggcagat gacgcgtgtg ccgggatgta gctggcaggg
    39901 cccccaccca tttctgcctc ccaccgtttc gtcaaaaaat cccaacatga ctaaacttta
    39961 accacaactg acgtcgcaag aattgtctgg ctgcgcagta cgcttcggag gtatgtctga
    40021 tgaagtatgt tgatggtttt gtggttgccg ttcctgccga taaaaaggat gcctatcggg
    40081 aaatggccgc taaggccgcg ccattgttta aagagtttgg cgcgcttcgt attgtcgaat
    40141 gctgggccag cgatgtaccg gatggcaaag tgaccgattt tcgtatggcg gtgaaagcgg
    40201 aagagaatga agaggttgtc tttagctgga ttgaataccc ttcaaaagag gtccgcgacg
    40261 cagctaatca aaagatgatg tcggacccac ggatgaaaga gttcggcgag tccatgccgt
    40321 ttgacggcaa gcgaatgatc tatggcggat tcgagtcaat catcgacgaa taggtagcgt
    40381 gacgggctgt gcggcgtgcc gtcagcccaa aaattcacaa atgttgctcg gcccagcgta
    40441 agaaatcttc tttcggtaat gccttgctgt agagccagcc ctgaccgtag tgcacgccat
    40501 gctggcgtag ccactcttct tgtttactgg tttcgattcc ctccgctacc attttcagtt
    40561 tcagtgtttt tgccatttcg atgatatgcg gcgtgacatt tttatattcc agcgcatcaa
    40621 cgaaagattt atcgatcttc agaatgtcga catccagatc ctgtaaataa cttaaacttg
    40681 aataccccgt accaaaatca tcaagataaa tttcatggcc cgcctcccgg tagcgagaaa
    40741 ttatcggggc gctggttttc ggatcggcaa actcgcgttc agtgagttca agcgcgatct
    40801 gtctgggatt aacctgatag tgattgatca tttcacgcag caattgcggg attttttccg
    40861 aggtgagcac ggtggattca agattgatcg aaatatgctg ctgtggatgc tgacgcagcc
    40921 agtcgcccat atcttcaaag acgcttctta taatcagtag cgtcaatggc tcagaaaggc
    40981 ccgtttgctg tgccagcgga ataaaactat ctggtgacaa ccaactaccg tctgtctgcg
    41041 gccagcgcgc cagtgcctca gcaccgacaa ttttgccatt ggctaaggag acaatcggct
    41101 gatagtgcac gcaaatatca cgattttcga tagcatcctg cagccgatga tgcggtgact
    41161 ggatgcggcg caaaatgcgc agcacaaaca tcgctgccag caggccaatc accaacccga
    41221 gcggtaacca aataaagact tgccgatgcc agcctttctg caacattttc gatgaagccc
    41281 aggtgataat tgaaatattc atctccggga agggcaggat atcgtaaatg attccattat
    41341 tttcgatatg ctcaccgggt gttttttgta gcctggtaat aattccctga gcaatttcat
    41401 cgctgctggt tatgacaacg ttatgggcat tgccaataat ggcggcatca atttgccatg
    41461 agctataggg aatgacatca ataaaggaag cggggtcgat catgacgaca taatgtgccg
    41521 ttcccatggc gaccatgtaa cggataatgc ctaaatcgtt atgtgatgtt aaccagacac
    41581 gataaccatc tttcgaaatt ttacctggct cggggaaggt atcgggcgga ctttcatgct
    41641 ccagagacga acactgggga acgttgttat cgatataaac cacttcctga atatagcgat
    41701 aactgtaaga tacccgacgc atttccatga gatgggcttc actacagtca gcgccttgcc
    41761 atctttccag ctcttgcagc gcatctttcc cttgtgtcgc caccttattg gcccgaatag
    41821 cgacgcggga ggaataggta tccagctctt caataaacga tgtttctacc tgctgatggg
    41881 ccagccagat gcttaagccg acaggcagca atactgaaag aatcagtact cccgaaataa
    41941 ggccgaccag atgtcgtgtt ctcaccagca tatccttatt aacacagtgt agggaaagta
    42001 tatctgattg tggaaaaagg ctggcaggag ataaggaggt agagctggta tttcatgaag
    42061 tgatgggtgg aaacgatcgt tgtcatttga ccaggcgcag agctttacgc ctggtatgta
    42121 atcctaactt aagatgttga ttcggcgcga gcgagggtaa aaatatcgta aaaagacaat
    42181 tcacctttac ggacgatcat tttttgcagt tgttccttat gaacatccgc gacacgcgga
    42241 gagttcaaga tggcttcaac cagcgcggca ggtacaaagc gcgtgttgta ccattcgccg
    42301 ttatagcaaa cgcggaaatc caacacgtcg atatcttcat agcggtagcg tgggtagaca
    42361 tcgaaaaaga agaaaccatt aagcaggata aataatacaa ccagtagcca gacagagcct
    42421 gaaagcgttt cggactgcaa catcaccgcc agggtggcaa aaaaagcaac gtacataccg
    42481 ataaacagcc ccggatgttt acggataaaa ctgatactaa agcggggttt gttgtcgcgt
    42541 ttttcgcggg tattcagaga ctcaatcgtt tcggtcagca ggcgttgtat ttcggtcatc
    42601 gtttgccttc gtggtatctg caactttcca gggaacaccc tattttaacg tgggaatagt
    42661 agagggaaag taacagatct gccacaattt tgcataacag ttgcgaaaaa ccgattacaa
    42721 ttttttaatt attctggctg gattaccgcc cacgacaacg ttgtccggga catcttttgt
    42781 gacaactgca cctgaggcta ccacgacgtt atcaccaatg gtcacaccag ggttaatgac
    42841 cgcgcgtccg ccaatccaga cgttattacc gatggtgacg ggtttcccca gttcagcacc
    42901 gctattacgt gctacagggt cgatgggatg tgttgccgtg tagatatgaa cgcctagtgc
    42961 caacatacag ttatcaccga tgcgaatagg gcagacatca agcatcacgc aatcgaagtt
    43021 ggcgaaaaaa ttattaccga gaaaaatgtt atagccatag tcacagcgaa acgttggctc
    43081 aatataagcc tctgtcacct gaccgaatag atcagcgaga atttgctggc gtaatgtgtg
    43141 ctcttccgcc agggaatgat tgtatcggtg aataagctga cgagcgcgca ggcgatcgcg
    43201 agataacgtc tcatctgccg agcgatacaa ctcaccagca atcatctttt ctttttctgt
    43261 gctcattact gacctccggt tgctgaaaat gcggcaacac agtaagacga aaagtcagtg
    43321 aagagaacgt tccc
//
LOCUS       DBFIFI010000019        39800 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.019, whole genome shotgun sequence.
ACCESSION   DBFIFI010000019 DBFIFI010000000
VERSION     DBFIFI010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39800
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.019"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            76..152
                     /locus_tag="N7B39_001025"
     tRNA            76..152
                     /locus_tag="N7B39_001025"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:110..112,aa:Met,seq:cat)
     gene            complement(226..1479)
                     /gene="amiC"
                     /locus_tag="N7B39_001026"
     CDS             complement(226..1479)
                     /gene="amiC"
                     /locus_tag="N7B39_001026"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HGU2421047.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            1711..3042
                     /gene="argA"
                     /locus_tag="N7B39_001027"
     CDS             1711..3042
                     /gene="argA"
                     /locus_tag="N7B39_001027"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HGU2421048.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKSCRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(3104..4930)
                     /gene="recD"
                     /locus_tag="N7B39_001028"
     CDS             complement(3104..4930)
                     /gene="recD"
                     /locus_tag="N7B39_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HGU2421049.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEESHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPTGDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRNPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(4930..8472)
                     /gene="recB"
                     /locus_tag="N7B39_001029"
     CDS             complement(4930..8472)
                     /gene="recB"
                     /locus_tag="N7B39_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HGU2421050.1"
                     /translation="MSDVAETLDPLRLPFQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAKWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     RNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVSAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMMRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLNFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQARGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYERHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIDLMDEMFAGMTLEEA"
     gene            complement(8465..11353)
                     /gene="ptrA"
                     /locus_tag="N7B39_001030"
     CDS             complement(8465..11353)
                     /gene="ptrA"
                     /locus_tag="N7B39_001030"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HGU2421051.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEALKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(11529..14897)
                     /gene="recC"
                     /locus_tag="N7B39_001031"
     CDS             complement(11529..14897)
                     /gene="recC"
                     /locus_tag="N7B39_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HGU2421052.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNALFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVSGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(14910..15233)
                     /gene="ppdC"
                     /locus_tag="N7B39_001032"
     CDS             complement(14910..15233)
                     /gene="ppdC"
                     /locus_tag="N7B39_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HGU2421053.1"
                     /translation="MSASLKNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLTSR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGRAGEMTRLH
                     CPNRQ"
     gene            complement(15218..15625)
                     /gene="ygdB"
                     /locus_tag="N7B39_001033"
     CDS             complement(15218..15625)
                     /gene="ygdB"
                     /locus_tag="N7B39_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HGU2421054.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCLLYAATGARVCLRLLEDNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(15622..16185)
                     /gene="ppdB"
                     /locus_tag="N7B39_001034"
     CDS             complement(15622..16185)
                     /gene="ppdB"
                     /locus_tag="N7B39_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HGU2421055.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGICTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEQVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSLVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(16176..16646)
                     /gene="ppdA"
                     /locus_tag="N7B39_001035"
     CDS             complement(16176..16646)
                     /gene="ppdA"
                     /locus_tag="N7B39_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HGU2421056.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISFIREGTLWCLVSSVAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIGFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(16830..17624)
                     /gene="thyA"
                     /locus_tag="N7B39_001036"
     CDS             complement(16830..17624)
                     /gene="thyA"
                     /locus_tag="N7B39_001036"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HGU2421057.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(17631..18506)
                     /gene="lgt"
                     /locus_tag="N7B39_001037"
     CDS             complement(17631..18506)
                     /gene="lgt"
                     /locus_tag="N7B39_001037"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HGU2421058.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(18657..20903)
                     /gene="ptsP"
                     /locus_tag="N7B39_001038"
     CDS             complement(18657..20903)
                     /gene="ptsP"
                     /locus_tag="N7B39_001038"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HGU2421059.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(20916..21446)
                     /gene="rppH"
                     /locus_tag="N7B39_001039"
     CDS             complement(20916..21446)
                     /gene="rppH"
                     /locus_tag="N7B39_001039"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HGU2421060.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(21799..21945)
                     /gene="ygdT"
                     /locus_tag="N7B39_001040"
     CDS             complement(21799..21945)
                     /gene="ygdT"
                     /locus_tag="N7B39_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HGU2421061.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            22131..22820
                     /gene="mutH"
                     /locus_tag="N7B39_001041"
     CDS             22131..22820
                     /gene="mutH"
                     /locus_tag="N7B39_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HGU2421062.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            22889..23602
                     /gene="ygdQ"
                     /locus_tag="N7B39_001042"
     CDS             22889..23602
                     /gene="ygdQ"
                     /locus_tag="N7B39_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HGU2421063.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            23740..23958
                     /gene="ygdR"
                     /locus_tag="N7B39_001043"
     CDS             23740..23958
                     /gene="ygdR"
                     /locus_tag="N7B39_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HGU2421064.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            24066..25106
                     /gene="tas"
                     /locus_tag="N7B39_001044"
     CDS             24066..25106
                     /gene="tas"
                     /locus_tag="N7B39_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HGU2421065.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLAPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(25138..26331)
                     /gene="lplT"
                     /locus_tag="N7B39_001045"
     CDS             complement(25138..26331)
                     /gene="lplT"
                     /locus_tag="N7B39_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HGU2421066.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHEQLPAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(26324..28483)
                     /gene="aas"
                     /locus_tag="N7B39_001046"
     CDS             complement(26324..28483)
                     /gene="aas"
                     /locus_tag="N7B39_001046"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HGU2421067.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDPQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEVEQHDE"
     gene            29069..30100
                     /gene="galR"
                     /locus_tag="N7B39_001047"
     CDS             29069..30100
                     /gene="galR"
                     /locus_tag="N7B39_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HGU2421068.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLLKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(30107..31369)
                     /gene="lysA"
                     /locus_tag="N7B39_001048"
     CDS             complement(30107..31369)
                     /gene="lysA"
                     /locus_tag="N7B39_001048"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HGU2421069.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMFDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVEPRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            31491..32426
                     /gene="lysR"
                     /locus_tag="N7B39_001049"
     CDS             31491..32426
                     /gene="lysR"
                     /locus_tag="N7B39_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HGU2421070.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLVRYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDELCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(32413..33105)
                     /gene="ygeA"
                     /locus_tag="N7B39_001050"
     CDS             complement(32413..33105)
                     /gene="ygeA"
                     /locus_tag="N7B39_001050"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HGU2421071.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(33234..34652)
                     /gene="araE"
                     /locus_tag="N7B39_001051"
     CDS             complement(33234..34652)
                     /gene="araE"
                     /locus_tag="N7B39_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HGU2421072.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLS
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(34967..35728)
                     /gene="kduD"
                     /locus_tag="N7B39_001052"
     CDS             complement(34967..35728)
                     /gene="kduD"
                     /locus_tag="N7B39_001052"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HGU2421073.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(35758..36594)
                     /gene="kduI"
                     /locus_tag="N7B39_001053"
     CDS             complement(35758..36594)
                     /gene="kduI"
                     /locus_tag="N7B39_001053"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HGU2421074.1"
                     /translation="MDVRQSIHSAHAKTLETQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIIGGIMPVTKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTGTPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(36881..38062)
                     /gene="yqeF"
                     /locus_tag="N7B39_001054"
     CDS             complement(36881..38062)
                     /gene="yqeF"
                     /locus_tag="N7B39_001054"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311728.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HGU2421075.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMTQSNGQTLVVDTDEQ
                     PHTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            38317..39546
                     /gene="yqeG"
                     /locus_tag="N7B39_001055"
     CDS             38317..39546
                     /gene="yqeG"
                     /locus_tag="N7B39_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HGU2421076.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
BASE COUNT         9853 a        10816 c         9673 g         9458 t
ORIGIN      
        1 ataacccgaa ggtcgtcggt tcaaatccgg cccccgcaac caatcaaatt tgatgaagta
       61 aaagcagtac ggtgacgcgg ggtggagcag cctggtagct cgtcgggctc ataacccgaa
      121 ggtcgtcggt tcaaatccgg cccccgcaac caattattga acaccctaac gggtgttttt
      181 ttgtttctgg tctcctataa aaaagcgcca ttcagcgcct ttttatcatc cccttctcgc
      241 cagggtcgcc ccatcggcaa aatacgcttt aattcccgca agaatagact ccgcaacttc
      301 ctgctggaag gtcgccgttt tcagtttacg ctcttcctca acgttactga taaacgccgt
      361 ttcgaccaga atggagggaa tatctggtgc ctttagtacg gcaaacccgg cctgttcaac
      421 ttgattttta tgcagcttgt tgattttacc gagcttattc agcaccgctt taccaaactt
      481 aaggctgtca gcaatggtca gcgactgcac catatcgaac atggtgtggt cgacatagcg
      541 gtcaccgctt ttgctcacgc caccaatcaa gtcggaggcg ttctgggttt gtgccagata
      601 ttttgccgca gtactggttg cacctttggt tgagagcgca aacaccgagg aaccgctcgg
      661 ctggcgactg gtaaaggcgt cggcatggat agagacaaac aggtcagcac gctgtttctg
      721 agcttttgct acgcgcactt gcaacggaat gaagatgtct tcattgcgcg tcatgtacac
      781 cttcatattg ccctctttct cgatcagaga gcgcaaacgg cgagctattt gcaataccac
      841 atctttttcg cgcgttttgt atttccccac cgcaccggag tcttcgccac cgtggccagg
      901 gtcaagcata atgacaatcg gacgatcgcg ccctgcttta cctggttgtg gaccactttg
      961 cgctggcggc acctgctttt cgaggtcgcc tttgttgtaa tcctccagca gcgccagcag
     1021 cgggtcctgc atatcctgtg cattggccgg atagaggtcc atcaccagac gctctttaaa
     1081 cccggcgacc ggcgcaaggg caaacagctg cggttttacg ttttgcttta attcaaaaac
     1141 catccgtacg gtttgcgggt caaattgccc gacgcgcgcc gacttgatga acgggtcgtc
     1201 tgcacgaatt tgtgccgcca tccccttgag taccgagttc aggtttacat cttcgatatc
     1261 caccaccacg cgttccgggt tactcaacgc gaactgctta tatttcagct gacgatttga
     1321 ttctaccgtc acgcgggtgt agctggacgc aggccagacg cgcaccgcca cgacctggct
     1381 gaccgcagcc agactgacct gacttacgct caatagccac atggcacccg cgccttgcag
     1441 taaacgacga cggctgattg cagtgttgga tcctgacatg cctctcccga gcaaaagaaa
     1501 tctaagctgt gtaacaagta aacgactaat ttgaccggaa actttagcga aagacgcata
     1561 atctgtcatc taataaacgg taaacattct ttttatattc acgccattac tgataaaaaa
     1621 gtcgctctcg cataaaattt acacttgcac cctgcgaaaa aacagaataa aaatacaata
     1681 aattcgaata ataatgcaaa gaggtgtgcc gtggtaaagg aacgtaaaac cgagctggtc
     1741 gagggattcc gccattcggt tccctatatc aatacccacc ggggaaaaac gtttgtcatc
     1801 atgctcggcg gtgaagccat tgagcatgag aatttctcca gtatcgttaa tgatatcggg
     1861 ttgttgcaca gcctcggcat ccgtctggtg gtggtctatg gcgcacgtcc gcagatcgac
     1921 gcaaatctgg ctgcgcatca ccacgaaccg ctgtatcaca agaatatacg tgtgaccgac
     1981 gccaaaacac tggaactggt gaagcaggct gcgggaacat tgcaactgga tattactgct
     2041 cgcctgtcga tgagtctcaa taacacgccg ctacagggcg cgcatatcaa cgtcgtcagt
     2101 ggcaatttta ttattgccca gccgctgggc gtcgatgacg gcgtggatta ctgccatagc
     2161 gggcgtatcc ggcggattga tgaagacgcg atccatcgtc aactggacag cggtgcaata
     2221 gtgctaatgg ggccggtcgc tgtttcagtc actggcgaga gctttaatct gacctcggaa
     2281 gagattgcca ctcaactggc catcaaactg aaagctgaga agatgattgg tttttgctct
     2341 tcccagggcg tcactaatga cgacggtgat attgtctccg aacttttccc taacgaagcg
     2401 caagcgcggg tagaagccca ggaagagaaa ggcgattaca actccggtac ggtgcgcttt
     2461 ttgcgtggcg cagtgaaatc ctgccgcagc ggcgtgcgtc gctgtcattt aatcagttat
     2521 caggaagatg gcgcgctgtt gcaagagttg ttctcacgcg acggtatcgg tacgcagatt
     2581 gtgatggaaa gcgccgagca gattcgtcgc gcaacaatca acgatatcgg cggtattctg
     2641 gagttgattc gcccgctgga gcagcaaggt attctggtac gccgttctcg cgagcagctg
     2701 gagatggaaa tcgacaaatt caccattatt cagcgcgata acacgactat tgcctgcgcc
     2761 gcgctctatc cgttcccgga agagaagatt ggggaaatgg cctgtgtggc agttcacccg
     2821 gattaccgca gctcatcacg gggcgaggtt ctgctggaac gcattgccgc tcaggcgaag
     2881 cagagcggct taagcaaatt gtttgtgctg accacgcgca gtattcactg gttccaggaa
     2941 cgtggattta ccccggtgga tattgattta ttgcccgaaa gcaaaaagca gttgtacaac
     3001 taccagcgta aatccaaagt gttgatggcg gatttagggt aagcgatgaa aatcgtcgga
     3061 tgcgacatgc gtaacactcg tacgtcgcat ccggcaatta cgtttattcc cgtgaactaa
     3121 acaacgccgc cagaccactg cgccgctcag tacgagtggc gattgccgca cttaatatgc
     3181 gctcatcggc atacagcgac agacggcgac gcgcgcgggt caccgcggta taaaccagct
     3241 ctcgcgttac taccggcgtg cgttggctcg gtaaaatcaa cgccgcatgg tcgaactccg
     3301 atccctgcga tttatgtacc gtcatcgccc acgtagtttc gtgctctggc aagcgactcg
     3361 gttgcacaga cttaatattg ccgtccggca tcgcaaacca gacgcgcgtc ccctgcccgc
     3421 gatccagcgc aataccgata tcgccattaa acaacccaag cgcgctgtca ttacgggcaa
     3481 tcatcaccgg tcggccttcg taccaacgag agtgcggatt acgatgaatt ttgcgcttct
     3541 gttgcataaa ctgctcaatt cgctcattca gtccagccac gccaaatggc ccttcccgca
     3601 gggcgcacaa aagttggtac tcattgaacg cctgaatgat taaatccggc tcggcacgcg
     3661 cttgcagcag atccagataa cgtccataac ccgcaagagc ttcctcaagc atcgcaatat
     3721 aatcttcgcc gctctgtaaa agccgttttt caatatcagt aaaatcctgc tgaaaaacgg
     3781 ttttcactgc cgttttatca ccacggttga tcgccgcagc taactgacca atgccagaat
     3841 cgctgccgaa acgatagctt ttttgcagca ggcagagact gtcgcgcaaa gatgccgctt
     3901 ctgtgccagt tcctgccgga acgtgagttc ccgtcaggcg gcttagctgc ccggcacgct
     3961 cggcggtaaa gccggcattg gcataagcgc agatatcgcc cagcacagcc ccagcctcaa
     4021 ccgaggccag ttgatcacga tcgccgagaa agatcactcg cgcatgatcg ggcaaggcgt
     4081 cgatcagtct cgacatcata ggcagatcga tcattgacgc ttcatctacc accagcacat
     4141 caagatgcag cgggttaccg gcatgatgac gtaaacgctg gctacccggc tgcgcaccca
     4201 gcaatcggtg caaagtactg gcatcttccg gaatacgttt cttttgttca tcggtcagcg
     4261 gtaactgtcg caaagccttg ccgagagatt cggttaagcg cgcggcagct ttacccgttg
     4321 gtgcagccag acggatacgg cagcgttcgc cgtcggccat ttgaattaac gctgccagca
     4381 acttcgctac ggtggtcgtt ttaccggtgc cagggccgcc ggaaatcacc gagatccgcc
     4441 gcgtcagcgc cactgctgcc gccacttttt gccagttaat ttcatcacca gtcggaaaga
     4501 gtttatccag ggtttgcgcc aacaaggctt catccacctc gatggcatga ttcacctcat
     4561 tgaagaagcg cgctaccgtc cgctcgttac accacatgcg attcaaataa agacgatcgc
     4621 cacagaggat catcggcgtg ggttcatctc cccggctgac cgcttgtgaa gccagcaagc
     4681 attcttccca attttgtagc tcaccgattt cactgacaca ggtcgccaac agcggatgcg
     4741 actcctcatt attttccagt cgtgaaagcg gcaaacaaac gtgcccctct ccggcatcat
     4801 gacttaacag tgccgccgcg agggtgacgg caggatgttc atctcccgcc acggtcaggg
     4861 caaactgcac gtccagcggg cgtagctgtt tgtgctccac agcttccagt aattgctttt
     4921 gcaatttcat tacgcctcct ccagagtcat accggcaaac atctcatcca tcaggtcaat
     4981 caacccggcg ttggggcggg ttgtgtaaat cccttgttgc ggatgttctt tatcaacacc
     5041 acgcaggaac agataaataa cgccgccaaa gtggcgctca tagtcgtaat cagcaatgcg
     5101 atggcgcaga taacggtgca gcgccagggt ataaagctga tattgcagat catagcggtg
     5161 tgcctgcatt gccgctgcca tagcctgttg ggtgtaagcc gaactatctt cacccaacca
     5221 gttggattta tagtcgagca ggtaataacg cccttcgtgg cggaacacca ggtcgataaa
     5281 gccttttaac atgccacgtg cctgcatgaa ctccagcggc gggcagccag cggatagcgg
     5341 gtcaaactgg cgaattagtg catcaagctg gctggcgatg agcggttcgc taatcggcag
     5401 gtaaaactcc atctccacct gtttatcgcg gtcggaaagc tgactcaggc taacacccgt
     5461 ttcattgaga ggtgcctgga ggacagccgt gatccactcg gtcaacaccg gttcccactg
     5521 cgattcaaag ccgccgagtt ccagtttttc ctgtacccag ttcgggtcaa ccggctgggt
     5581 aaaattgagg tcttcaaaca aactgtgcaa gaatgtcccc ggtgacgcac cgcgcgggaa
     5641 ctgatgcggt gttaacgtcg gttcttcaac gacgctgacc acgcctgcgg catcaacatc
     5701 cagccgtggc atcagatcct gagcgatacc gtgaccacgc tgctgcaagc cggagtagct
     5761 ggtgacacgc cagttatcgc cgggcaatcg ttgtagcgtc ctcgcattca gttctgcagt
     5821 taaagcatca ttaacctgcc agggctggtt atcaccagtt tgtgccgttt gccaggcaat
     5881 atcatcatcg cataacgctt caatacaggt gcgaagccct gccgcatctt gcggttcccc
     5941 tttttgcagc aaacgcccga gggcactttg gtggacgtcg gtgtcacctt ttttatcgcc
     6001 acgacggcgc accagcggtg caacgccgag actgcaatgc caaaccgaac gcgtcagcgc
     6061 cacgtaaagc aaacgcagat cttccgccag acgttcgacc tccgcaagat cgacgctttc
     6121 tggcgcagcg ttaagatcca gaacagcctc aaacgagtgg cgatcgtgat aaaacgcctg
     6181 atcctggacg cggaaattgg taataaatgg caaccagacc agcggatatt ccagcccttt
     6241 tgatttatgg atcgtgacaa tctgtaccag atgtttatca ctttcgaggc gcatttgttg
     6301 gctggaggca ttactgtctg gctcgaggat atgttgcgat aaccagcgta ccagcgcatg
     6361 ttcactttcc agctgcgttc cggcttcttg tagcagttcg ctgatatgca agatatcggt
     6421 aagacgccgc tcaccgcctg ccgttgccag caagttttct gcaatgttac gcgccgacat
     6481 cagcgcccgc atcatcggca taacgccacg tttgcgccag atttgccgat aaccatcgaa
     6541 ctcttcgacc accgcgtccc acgcatgttc gtcattattc agcgtttcga tatccagcgc
     6601 gttcagcccc atcattgacg ttgccagcgc actacgcagg gtgttctcac gttcgggcgt
     6661 catcaccgcc tgcaacaacc aaagcatttc ctgcgcttcc agagtttcaa aaacactgtc
     6721 gcggttcgaa aggtaaacgg aagggatttc cagcaacgtt aaggcatcgc gcacctgggc
     6781 ggcctcctgg cggctgcgca ccagcacact gatgtccgaa gcacgcaccg gacgcgcgtc
     6841 gtcgccgttc atcagcaacg cttcgccccg ctgtccggct tgtagccagt cgcgaatttg
     6901 cgcagcactt acctgcgcca tggtactttg atagtcgcca acgccgcaac tttcgccttc
     6961 catcagccac attttcatcg caggctgcgt ttcaccttta aagacaaaac gtaacgcctg
     7021 atttttcccg gctgatttca ctggaataaa cggtatttcg cgaaacatga acgcgtcatc
     7081 agtctggctg aaaagcttgt tcacactgtt caccattcca ggtgcggagc gccagttggt
     7141 gtctaaagtg tagtgggcgt gaacttcact acgcgccttc atataagtga agatatccgc
     7201 accccggaat gcatatatgg cctgcttcgg gtcgccaatc agcaacaatg cggtttccgg
     7261 ctgatggtgc cagatacggc gaaaaattcg gtactgctgg gggtcggtat cctgaaattc
     7321 atcgatcatt gccaccggga atcgcgtacg gatcgccgct gccaacacct caccgctttc
     7381 gctacgcagc gcggaatcga gccgacttaa catgtcatca aaacccaatt cgccacggcg
     7441 gcgtttttca cgcgctactg tttcgcggat ctcagccaat gcgcgggtga tcaccagatc
     7501 gcggatcgac aatggttctg caagcagttg atcgatcgcc tcaaacagtg gatgtcgcgg
     7561 ggtttccccc ccggccttcg tgcgatcttc taagaaacgc tgggagaatt tttccagcga
     7621 ctccggcaac tgataactgt ttctctcttc ttctgcccag gcgctgatct tgtcgatcca
     7681 tttagcctga ttgctacggt taaacttgcg tcgatcaata ccagaagatt cgatcagcgc
     7741 atccagttca cccactgcgt cgcgccactg ctgttttacc gtatcaatac gcgccacaat
     7801 ttgcgcgtga cgggaagcca gcgtttcatc atcgggcggc ggtgctttga taaccggcgc
     7861 ttcaccttgc agataacgat taatatcgcg cagcaacgcc tgcggccctt tccaggtttc
     7921 aaagacgacc tgggctattt cacgcggcag cgggtagcag tggcgacgcc agaaatcggc
     7981 gcaggcctgg taacgtagca gagactcatc ttcaatcagc tgctgctcaa acagcatgcc
     8041 ggattcaaag gcattcaggt tgagcatgcg ctggcaaaaa ccgtgaatag taaagactgc
     8101 cgcttcatcc atctgccgtt cggctaacaa caaccacttc gcggcttgcg ctttatcgtc
     8161 gatctcttcc agcagacgtt cgtacagtgg attgtcggtg gtttcacgca gacaggcgat
     8221 gcgcaactca tggatattgc tacggatacg accacgcaat tctgccgtgg cagcctcggt
     8281 aaaggtgacc accagcagtt cttcaacggt cagcgggcgg ggaaaggcgg cggaaccgcc
     8341 tagtccaagt aacaggcgca aatagagcgc cgcaatcgta aaggttttgc ctgtgccggc
     8401 agaggcttca atcaggcgct cgccctgaaa gggcaagcgc aaaggatcta gtgtctcggc
     8461 gacatcactc attcttttca ctcatcaggg gcattgtttg ctgcaacgcg ctgacgttct
     8521 cccacacttt ccagccttca ggatgcacat attcggcttt cccgttctgg ctgccggaaa
     8581 tctgcgacag aatagccatg ccttgcggct cgaccaccgc ctgatggaag aaatcagcaa
     8641 gtttttgcgg cgtcagcagt tttatctggg ccacgatttt atcacgcgaa tcgaagcgca
     8701 tattgccgcg atcgaaatct ttacttaact tcaatgcttc ttcgccgagc gtttgcggtg
     8761 cctgcagcat ctgggtaatt accgcctgct ggatttgcgc aaactcatct ggcttcatcg
     8821 ctcgcaattt tgcctctgcg gttgggaaaa acgccttgta acgctcccac aagaatgaag
     8881 gctgtttatc attgctttgc aaaaggaagc ccatgcccca ctgacgcccc acgctcattg
     8941 gaaacgcaaa cacggcatag cccaattgtt cttcggtacg caactgattg tagaaccacg
     9001 gctgtacgat ctgccccaac agagagctat aggctgagct tgtgtattca tcgtagccag
     9061 tcggtacaaa taccgctgcc agtgcggagt cggtgctgtt accggctttt tcaaagatga
     9121 cggattgttt tttatcgacc actacatctt tgtttcgaca ccactctgaa ccatcagcgc
     9181 ccaactgttt ttgcacatcg cgtgccagcg ttgttgcctg ggcctcggtc atgttgccga
     9241 taaccataaa ctctggtcga gcccctgatt ttaaggcgtc acgataagcc agcacctctt
     9301 tcaacgtaat ggagggcaaa attttacgcc gttcatctcg cgagaagtac ggcacttgcg
     9361 agagcatctg cgcgggcata atcgcctgct caaacgcttt acccttttct gcggaatcca
     9421 tcatctggtt ataccaggac ttcgcctgct caagctgatc ttccgtagcg gtatagctaa
     9481 agtatccctc gagcaatgcc tggaacagct gcggcagacg ctgggtgtaa ccattagcat
     9541 taaccataag gccgttgtta gcgttggtgg aaaaacttat gccaccaacc gacgcctggt
     9601 tgcttaactg atcaagcgcc agccctgcga gataatcatt gagcgcaaac atcacctgat
     9661 tgcgggcgct gtccatggct ttcggattac gcaaaatcag gctgacatca gctttgggtt
     9721 cgctggaaaa ataacggctt ggcgcataca ccacgcgcag attcgactca tcaacaatca
     9781 gctctggatg gtcatatttc ttctctgact taatcagcga gaaatcgtca ggaatatagg
     9841 ggttaagctc cggtaaggag agcgcaatat tggcagcttt ttgctgccag tcagcgaaag
     9901 tttgttcgct gattttatcg acctgatacg gcgcatcgac aaaataagcc gttttattgt
     9961 gcggctcttt cgggctgata taccagatac gcgcattctg cggcgtcatc atcgccagac
    10021 gttcttttac tgctttagca tcgtaccgat cggcaatatt gactgcatcc agcgtatgct
    10081 caacaggaac gcgaatcatg gtatctgcca gccattcgac gtaatccata tcacgggtga
    10141 ttgacggata acggaagtcg atatccagca cattcgccag ttcatcgaag tattgtttat
    10201 cgatcccttt ttcacgtaac aaattgaggt agctaaaaat agccgccaca acctgatcgc
    10261 gattcgccag acctttatcg gttaaagacg cagagatcgc taatacgccg ctgttgccgt
    10321 tgacgatagg atcggagttg gcgctaatgc cctcaactaa cccctgcttt tgcagccagt
    10381 cagacagtgt acctgggctg cgattaccaa tcagataggt aatcaattca tccgttttac
    10441 tgcggaactt cgctgagttg ttatcgatgc gaaactcaac gcgcagaact ttacgcggca
    10501 gcgctgggac gtaatgaatg ataatgccct tttgcgcgtc ggtgactacc ggcacggtga
    10561 tttccggttt tttgctctct ttgttcggca cgcgaccaaa agtgtccgcc gccatttttg
    10621 ccaactccgg cagcggttta ttactgtaaa taaccgcctt catcaaattg gcggaatagt
    10681 acttctcgtg gaaatctttc agcgcctgct gcaccggatt accaggtttg tcgcttaaag
    10741 tttcgaggtt accaccagaa aactttgaac cggggtgtgc cgggttaatg gtttctgcgc
    10801 tgacctgtgc catgcgcatc ccgtcacgtg tacgcgccat ggttaattca gcgttcactg
    10861 cattacgctc acgttcggcg tatttcttgt cgagcaaggg ttctgcaata gcatcggcca
    10921 ggcggtctac cgcaccgggc aaggcgtcgt tctcaacttc cagatagaaa gccgtgcgat
    10981 acggcgcagt gctggcattg tgactaccgc cgtgcatttt gagatattcg gccagactgt
    11041 cagcctgcgg gtactttttc gaccccatca gactcatatg ttcaaggtaa tgtgccagcc
    11101 cctggtacgc ctcgggatct tccagcgacc caacgggcac caccagcgcc gatagcgatt
    11161 taactgcctg cggatcagaa accagcaaga ccaccatacc gttatccaga cgtatagcct
    11221 gatactggcg gttatcttta tcacttttac ggatggtttc ctgaatcggc tgccatcccg
    11281 tttctgcctg acttaagggt gcccaaaggg caactaacaa caataatgct ttgaaccagg
    11341 tgctgcgggg cattcacgga cctcataatc aactttattt tctggccaga ttcaacaggt
    11401 taacgcaaat catctgccag aatttaatct tgtgctgcac gagtcagcct atgtttatat
    11461 aaccatcagt ccgtgactgg tgcgcatcat aaagtaagcg gatagattgc gcaattttta
    11521 tacagcgctc atgactgatt aaagcgaaac agcggtaaca ggaaacgttg cgactgttca
    11581 acgattgcct ccattgtctc tggtgttaat tgccgccaga gcctttgata ccagatatca
    11641 tcaccttcgc cactcaccat catgttgcct tcgtaagcct gaaggaattt cgtacgggct
    11701 ttttgcaacg tggaatcgtc atccagcatg gcatcgtttt gcgcgtcata acaggttttt
    11761 agccacgcgc cgccactttc aggtaacacc agcaatggcg cggacatccc ttcacgatac
    11821 ccctcaatca gttgtgagag gtaatgcaaa gcctgttcgg ctgcaagcgg cggaaaacgc
    11881 cactcgccgt ctttgcgtag aaaaaggcga ctttcaccat taccaccgct ggcacagtag
    11941 acaaggtgtt ccagccaaag ttgcattccc tgcgccacac ttaataaaga gggacgccag
    12001 cgcaacaagc catccggctg cacctgcggc aaccagccag ttatctgcac accgttgcaa
    12061 gcgagatcaa tttccatact ctgccccggc tggcgacagg caatgactct gtcggcaagc
    12121 tgctgcatct cctggcactg tgtttcccag aaaatttcac caaaagcgcc atacggtaaa
    12181 tcccctgccg cccggaagcg gcggaacaag cgttcggcat catcctgctc aaccagtgca
    12241 ttcaataact gctgattgat ttgataacgg ctaagtcctt ccagaataaa tggctcggtg
    12301 tcggggattt cgctgtcttc agtacggaag ttcacctgca aacgcatctg gaagaacgcc
    12361 cgcaccggat gtgcccagaa tcgttgtagc gtttccagcg gcacggtttc cggtaaggta
    12421 aacggcagcg gctgaacaaa ttcagaatgt gctttaccag cctggctggc cgcaggtagc
    12481 cattcacgag catagctttg tcgttcgcct ggctggtagt tttgcggatc aaacggcatc
    12541 cgggtatgga ggcaagtaag atgcgctttt acccttgcct cgctttcatc acagttgagc
    12601 gcttcatcgc ccggtagata atgactttgc ccgatgtagt cgatcagttc ctgcaccagt
    12661 accgacggga aacgttcact gttatcctga atggaacgcc cgatatagct gatatagagt
    12721 ttttgctgcg cggaaattaa cgcttccagg aacaggtagc ggtcgtcatc gcgacggcta
    12781 cggtcgccac gcttcggttt ctggctcatc aggtcaaagc ccaatggcgc aagctgacgt
    12841 ggataaacgc cgtcgttcat tcccagcagg caaaccactt tgaacggaat tgaacgcatt
    12901 ggcatcagag tacaaatgtt aaccggcccg gcgagaaaac gctggctgat acgttcttga
    12961 tccaggcgct gtgccagttc atcacgcaat agtgacagcg gcaccgcatc gccatactgc
    13021 gcacctaaac cttcggcgat aatcgcctgc cattgttgtt cgatcagcgt catcgccgct
    13081 tcggtttccg catccggcag gaagagggcg ttgagcatat cgcgacaaac cggcaaccac
    13141 tcttccagcg gacgctcctg tgccagcccg cgacgccaga tgtttagctg cattagcagt
    13201 gaagccagat gccccaccag ttctgcaatt aagccgctcg attcatcata aggtagaacc
    13261 gattgccact cgccctgcgc gctctccatc gcgtagccca acaacatacg cgtcaggcca
    13321 aatcgccagg tgtgttgtcc ggtggcgggg agttccagct cgcgaacgtt gtcgtcatct
    13381 atcccccaac gaatgccgga ttcgttgacc cactgacgta aataacgcag cccttcttcg
    13441 gtgatgtcaa accgcgccgc cagcaccggc acatccagta atgccagcac gtcttccgac
    13501 acaaagcggc tgtctggcaa cgataacagg ctgataaacg cttcaagtac aggatgcgac
    13561 tgccgcgccc gacggtcgga aatggcgtaa ggcaggtaac gatccgcagg tgcgctacca
    13621 aacacagcct gaataaacgg actgtagctg tcgatatcag ccaccatcac gatgatgtcg
    13681 cgcggagtaa gtgtcgggtc ttcttccagc atcgccagca ggcgatcgtg taaaacttca
    13741 acttcacgct gcgggctatg gcaaacgtgg aaggtgatac tgctatccag tggatcaagc
    13801 ggacgtttgt tatcgctacg ggaaaactct tcgatgttca caccagcaac ggcgcggttt
    13861 tccagttcca gaatgtcaga ctgaatgtta tgcagcaggt tatctggcgt cacatcgaca
    13921 aaggcgtcca gctcctggct gctctccagg tcagaaagga gataaatgta gtcgcgccca
    13981 agcttgcccc atgaggccag cagcgggttg ccgacatcct gttcaccatc gctgttaaag
    14041 agctgcccgg cattttcgct gtcgcgaaat agcggtaatt cgcgatcttc aaaactgtgt
    14101 cggcgctggc gagtcagtag tttcgccaga taagcaggat ctttaatgtc gccccagtaa
    14161 taacggcagg ggttggtaaa caggagatgg atttcaatat gtttacccag cgcctggagc
    14221 gcctggagat aaacaggcgg taacgcggaa ataccgcata taaagacgcg cgaaggtaac
    14281 cccggcgggc aggtcgtcgc ggactccagc gtttcgataa agcgctgata gagattggcg
    14341 cggtgccagc gcggttgtcc gagttcatgg gtatattcca ccagcgcctt ccacaacggc
    14401 gcttgccagg cctgtgcctc tcccagccct tcaaccaaat gtcctgtttc ccactgtgcc
    14461 agccagtccg gacgatagac cagatactgg tcaaacaggt ccgccgcttt tgaggaaagc
    14521 tggaacagct ttcgcttgtc gctatcgtca gtcagataat gccgcaacag ggtaaagtct
    14581 tcgcgctcca gcaattgcgg cagcagagtc atcagtttcc agctcatgct ctgtttgtta
    14641 aaggcgctct ctttggggat ctccggtaac acccggacga acatatccca gataaagctc
    14701 gcaggcagcg gaaaatcaat gtttgccgca ataccaaact tttgcgacag ggtcatttgc
    14761 agccactgtg ccataccggt actttgcacc agaatcatct ctggttcgaa aggatcgtcc
    14821 agccgttcgc gttcgacaat aaactccatc aacgcttcca gcacgtccag acgattggaa
    14881 tggtagaccc ttaacatagc ggctcctgac tactgacgat tcgggcaatg caggcgggtc
    14941 atctcgcctg ctctgccccc gggtgaaact agcgtaacgc tgatgctgac acatcccgcc
    15001 tgcgatgtct gcattcggtt gacctgccag ttggcaggtg gcgaaatcgc gcgcaattgc
    15061 gtttgctgcc aaccatgccg ccagagctgt tggtactggt ttctgctggt aagactgttc
    15121 attaatgttc gctggaaacc cgataacgca gtgacaatca tcaccatcaa caccatcgcc
    15181 aacattacct ccggcaggct aaagccttgt tgattcttca gggaagctga cataacgccc
    15241 cctctttcag cggacaaaaa tcgctccagc cgcgtggcga aaaaacaatt tttccatcaa
    15301 tgacttcgcc tgttcgccac aacgaaacgc cttcataacc ggcaattaat aaggcttcat
    15361 tatcttccag taaacgcaaa caaacccggg caccagtggc agcgtacagt aagcactgaa
    15421 ctgcgggctg cgtctgccag gagtgcattt tcccccacgc caacgccgac tgaacgatgg
    15481 cctggcggcg cagtgactga ctttccatgc tcacgcgaga ggcaaaactg cgatcctgct
    15541 ggctcattcc ttgtaatagc aagctaccca aaaccagcag catcaggacc agagccagtg
    15601 acgaaacacc cttttcgcgg ttcacaggtt gaatcctgtc acgctatagc tggcatccac
    15661 cacggtttgc ggttcagact tactggcagc acgcatatta accgtcaaca ccagcgagaa
    15721 gccgctgaca tcctgacgta cgacctgaaa agtgtcgata atgatggcat ccggattagt
    15781 gactttatcc cagcccttac cttcacagga tgtcgcaccg cgtagcgttt ccaacacttg
    15841 ctccttcaga cgaaatccaa tctggtcgga ctcttttacc ggttcgcgat cccagatacc
    15901 gttactgttc gcatcccact gcacaatgat acagtcaccc tgtccgacaa tttccagccc
    15961 ttcgccggta cagatgccat gacaataacc cgccctctgg agatgcttcg cgacggtaaa
    16021 tacccgcagc cagatttcat cttccagtgc cagcttacgg gtgctcgtta aactttcacg
    16081 ctgtaacgca ggcagaaagc gtgcagcccc caacaacaat acgctactga tcgccatagc
    16141 aatcaacact tccagcagag aaaaaccttg ctcttttaca ggcatccttc tgtttctcct
    16201 tgctgacaaa gccggagtct tccccacggc gaaaccacca gccaccactc gcccgttgag
    16261 tttttgaagc caatatgccc ggcccatgcg gtattgcgca ggccaaagaa agcaagcgaa
    16321 ggtgtcaggt cgctcatttc gacttcgggc cagcgtggca caaagaccaa tggtgaactg
    16381 ccatgacagg tattcgcccc agcaacggaa ctcacaaggc accataacgt cccctccctg
    16441 ataaaactga tactgtggtc gcggttatgc cagttggcat cttcacgtaa atagagcaaa
    16501 tagtcccgcg cctggctggc ggtttgccaa agccgttgcg actgctgcca gtattgccag
    16561 ccatagagtc cacttgcgct tagcatgacc aaaatcagca tcgcgaccag cgtttcaatc
    16621 agcgtataac cacgttgtgt tttcatgccg gcagtatgga gcgaggagaa aaaaagacga
    16681 gggccagttt ctatttcttc ggcgcatctt ccggactatt tacgccgttg caggacgttg
    16741 caaaatttcg ggaaggcgtc tcgaagaatt taacggaggg taaaaaaacc gacgcacact
    16801 ggcgtcggct ctggcaggat gtttcgtaat tagatagcca ccggcgcttt aatgcccgga
    16861 tgtggatcgt agccttcaat ctcaaagtct tcgaaacggt agtcgaagat ggattcgggt
    16921 ttacgtttga taatcaactt cggcagcgga cgcggttcac ggcttaattg cagatgagtt
    16981 tgatccatat gattactgta caggtgcgta tcgccaccgg tccagacaaa atcacccact
    17041 tccagatcgc actgctgcgc catcatatgc accagtaacg cgtagctggc aatgttgaac
    17101 ggcaggccga ggaagacgtc acaggagcgc tgataaagct ggcaagagag tttgccgtct
    17161 gccacataga actggaagaa tgcatggcac ggtgccagcg ccattttatc cagttcgcct
    17221 acgttccacg ctgaaacaat aatgcggcgg gaatccgggt cgtttttcag ctggttcagt
    17281 accgtagtga tctggtcaat atgacgacca tctggcgttg gccaggcgcg ccactgttta
    17341 ccatacactg gtccgaggtc gccgttttca tcggcccatt cgtcccagat ggtgacattg
    17401 ttttcgtgta gataagcaat gttagtgtcg ccctgcagaa accacagcag ttcatggatg
    17461 atggaacgca ggtggcaacg tttagttgtc accagcggga atccatcctg caggttaaaa
    17521 cgcatctgat gaccaaaaat ggaaagcgtt ccggttccgg tacggtcgtt tttctgtgtg
    17581 ccttcgtcga gcactttttg catcagttct aaatactgtt tcatggttcc tcaggaaacg
    17641 tgttgctgtg ggctgcgacg atatgcccag accatcatga tcacacccgc gacaatcatc
    17701 gggatggaaa gaatttgccc catgctgatg tactgcaccc aggcaccggt aaactgcgcg
    17761 tcgggctggc ggaaaaactc aacaatgatg cgaaacgcgc cgtagccaat caggaacaag
    17821 ccagacacgg ctcccatcgg gcgcggttta cgaatataca ggttgaggat aataaacagc
    17881 accacacctt ccagcagcag ctcgtaaagc tgtgatgggt ggcgcggcag cacaccgtaa
    17941 gtgtcgaaaa tggattgcca ctgcgggttg gtttgcagca gcaaaatatc ttctgtacgg
    18001 gagcctggga acagcatggc aaacgggaag ttcgggtcaa cgcggcccca caattcaccg
    18061 ttaataaagt tgcccaaacg cccggcacca agaccaaacg gaatgagtgg tgcgataaaa
    18121 tcagaaacct ggaagaagga acgtttagta cggcgggcaa agataatcat caccacgata
    18181 acgccaatca ggccgccgtg gaaagacatg ccgccgtccc agacacggaa cagatacagc
    18241 ggatcggcca taaactgcgg gaaattgtag aacagaacat aaccaatacg tcccccgagg
    18301 aagacgccga ggaagcccgc atagagtaag ttttcaactt catttttggt ccagccgctg
    18361 cccggacgat tcgcccgtcg tgttgccagc cacattgcaa aaatgaaacc caccagatac
    18421 atcaggccgt accagtgaag cgccacgggt cctattgaga aaatgaccgg atcaaactcc
    18481 ggaaaatgca gatagctact ggtcatctgt caccacaagt tcttgttatt tcgctgaaag
    18541 agaacagcga ttgaaatgcg cgccgcaggt ttcaggcgct ccaaaggtgc gaataatagc
    18601 acaaggggac ctggctggtt gccggatacc gttaaaagat atgtatatga tccgcgctat
    18661 aaccctccgc gaatcagccc gcccatgccg cgacgctcca taaaggctgc aacctgatgg
    18721 cgaacttcgg tcgccagttg cgcttccaga ctacgctgcg caagattttc tgcttcggca
    18781 taatcaatgc gccgcagcag gtattttacc cgcgctacag aacgtccgtt catagacaga
    18841 tggcgatacc caagcccaat gaggattgcc acgcacatgg gatcgcccgc catttcaccg
    18901 cacaaacgga gatcgattcc atgtatttcc gcttcccggg cgatcatcgc cagagctcgt
    18961 aacattgcag gatgaagact gtcataaatg ttcgccaccc gggtattgtt gcgatcaacg
    19021 gccagaatgt attgagtcag atcgttggtg ccaacagaga tgaaatcgac ccgctttgcc
    19081 agatgcggca gcataaatac cattgacggc acttccagca tgatgccgat acgtggtttg
    19141 ggaatttcgt aaccgatcat ctcctcgact tcacgtccgg cacgttcaat caggcggcgt
    19201 gcttcgtcaa cttcatcgag gcttgtgacc atcggcaaca gaatattcag gttgcccgta
    19261 gcggcattag cacgcagcat cgcccgcacc tggatcaaga agatctccgg ctgatcgagc
    19321 gtaatgcgaa tcccacgcca acccaggcat ggattctctt cgctaatcgg catgtaaggc
    19381 agctgcttat ctgctccgac atccagcgta cgcaaggtga cgggtttatc attgaacatt
    19441 tgcagcatcc cctgatactg cgccacctgt tcttcttccg acggaaaacc actttgcagc
    19501 atgaatggga tttcagtgcg ataaagaccg atgccatcaa tacggctgcc cagtttttct
    19561 tcatgttccg ggcttaaacc agcattgagc atgactttta tacgctcacc gctttttaac
    19621 tgcgcgggta aattgacgtc atcttccgcc agacggctaa gctcaatctc ttcactaatt
    19681 agccgctgat attcttgcag cagtaccggc tccggatcga ccagcaattc accgcgatag
    19741 ccatcgacga tcagcgtccg acgatgcagc accgaaggct gaatatccgc gcccatcacg
    19801 gtagggatcc ccagcgcacg taccatgatc gcagcatggg agttggctgc gccatctcgc
    19861 acgacaacac cgactaagcg atcctggggc agctcagcaa gagttgtcgc tgacagttca
    19921 tctgccacca gaatgaaacg ttccggccag gcgttcggcc cttgattagc gtcatcaaga
    19981 tgaaacagca atcgctgacc cagcgcacgt aaatcgccag cccgctcttt gagatagtta
    20041 tcgcttagcg cggcaaactg ttcggcaaat ttttcaatga ccgtttttac cgcccattct
    20101 gccaccgagc ctttatcaac ctcggcaaac aattcgcgac gcagccgggt gtccgaaagc
    20161 aggtgcgagt aaagatcgaa aatagccgcc gtttcttttt gtgcaccggc ggcaaagcgt
    20221 ttgctgtagc ggcgaaactc gtttgccgct tcttccagcg ccccggtcag tcgttcgcgt
    20281 tccagagccg gatccagcgt tgatgcctga tacacctgtt ccattaaagg taacgtggca
    20341 tcctgccagc cttcggcaat cgctacacca ggtgctgccg gtaatgcgcg gattcgcgtc
    20401 tggcgatatt gcccaaacaa ggcagtcaac tgcgactgag aaagaatcgc tgccatctgg
    20461 gtggcaagcg tcaccaggaa ggattcttca ctttcgtcat actggcgcaa ctctcgttgc
    20521 tgtaccacca gtacaccaag caactggcga cgttgaataa ttggtacgcc taaaaacgcg
    20581 cggaaacgtt cttcttttac ggaggggatg tatttgaagc tggggtgctt ttgcgcatct
    20641 gcaaggttta tcggttccgc cagcctgcca accaggccga cgatcccttc atcaaaggcg
    20701 agcgttacag tgcgaccgcg tggttttttc agcccccggg tcgccatcag gtagtaacaa
    20761 cgtcgatcat gatcggccag gtagaccgaa cagacctcgg tatccatcgc aagacagatg
    20821 tcggtaacca gaatatttaa cgcctcattc aggcgtggtg cgctggctac cttttcgact
    20881 atttcgcgca ggcgagtgag cataattggc gtgacttaac ctcttttacg tcgataagca
    20941 gatgcgtttt gtggttttgg cgtattttcc tgcagtgaca tcaccacact cgcgaactct
    21001 ttcattaccc tacggtagac atcacgttta aatgacacca cctgtctgac cggataccag
    21061 taacttaccc atcgccagcc gtcaaactct ggtgtactgc tggtttgcat attgatttct
    21121 gcatcgccgc tcaccagctg caagagaaac catttttgtt tttggccgat acaaaccggc
    21181 ttcgtgtccc aacgcaccaa acgtttcggt aatttgtagc gcaaccagtt acgcgttgaa
    21241 gcaaggattc gaacgtcttt gcggcttaat cctacttctt caaacaattc acggtacatc
    21301 gcctgctctg cggattctcc ggggttgatt ccgccttgcg gaaattgcca ggagtgctga
    21361 ccaaatcgcc gggcccacat tacctgcccc tggcgattac aaatcacgat acctacgttt
    21421 gggcggtagc catcgtcatc aatcaccgga ctacctcaaa ataaagcttt atatacgaat
    21481 gattgtttca tactccagga agacggtaaa ccactctctg cagggcatta cacactaaca
    21541 acaattgaat aactcacagt tatgtgcaga gttataaaca gaggaagggg tggatagccg
    21601 tttttattca ccttttctgt ggatagagtt gtgaagaact acggaattac tacgggaaaa
    21661 cccggagcat tctgaataag cccgatatag acattttaaa atattcatta ttagtcaatg
    21721 cattacatgt aatttaagta atgaaaaaga gtaattcgtg acccaggtca cacctctcat
    21781 ttacgggttg gcgaaagatc aaccaatgcc gtatttatcc acagaatgtg ccactaagtt
    21841 aagcactgaa ccactaaaaa ctggagtttc gtcgcacgtc aaggctgtaa atggaaacag
    21901 tagtggaggt ttttcacagt tatcccagct ttctgtggat aacatggtgt aagatcctgt
    21961 ttattttcag tgaccagatt tggaaaaccc gttgcagtgt tgcgcaactc gattaccggc
    22021 aacctaaaaa gcaatataaa tcagataatt gaacaacgca tgtggaaaaa gttacactgc
    22081 gaatattcgg cacataattg ctgtttgttt tttaaccaag gtatcatgac atgtcccaac
    22141 ctcgcccact gctctctcct cccgaaactg aagaacagtt gttagcgcaa gcacagcaac
    22201 tttctggtta tacattggga gaactggcgg cacttgccgg gctggttacg ccagagaatt
    22261 taaaacgcga taagggctgg attggcgtgt tactggagat ctggctaggt gccagcgcag
    22321 ggagtaaacc tgaacaagat tttgctgctc tgggcgtgga acttaaaact atccctgtgg
    22381 atagtcttgg tcgtccgctg gaaacaacat tcgtttgtgt tgccccgtta acgggcaata
    22441 gcggagtgac ctgggaaacc agccacgtgc gccacaaact caaacgcgta ctgtggatac
    22501 cggttgaagg cgagcgcagc atcccgctgg cgcagcgtcg cgttggatca ccgttgctgt
    22561 ggagcccgaa tgaagaggaa gaccggcagc tacgcgaaga ctgggaagaa ttaatggata
    22621 tgattgttct cggtcaggtt gagcggatta ccgctcgtca cggggagtat ttacagatac
    22681 gaccgaaagc agcgaatgcg aaagcgctta ccgaagccat tggtgcacgg ggcgaacgga
    22741 ttctgacgct gccgcgcggc ttttatttga agaagaattt caccagtgca ctactggccc
    22801 gtcattttct gatccagtag ccatcgcttt gacctgccgc tttccgggca tataattacc
    22861 gcttcatttt tttggcaggg ctttttagat gttatttgca tggataaccg atcctaacgc
    22921 ctggcttgcg ctcggtacgc tgacgctgct ggagatcgtt cttgggatcg acaatattat
    22981 tttcctttct ctggtggtgg caaagctccc cacagcacaa cgtgctcatg cgcgccgtct
    23041 ggggttggcg ggagccatgg ttatgcgtct ggcgctgctg gcatcaatcg cctgggttac
    23101 gcgcctgacg aatccgcttt ttacaatatt cagtcaggaa atttctgccc gtgacttgat
    23161 tctgcttctg ggtggcttgt tccttatctg gaaagccagc aaggaaatcc acgaatccat
    23221 tgaaggtgaa gaagaagggc tgaaaacacg cgtttcatca ttcctcggcg ctatcgtgca
    23281 gattatgctg ctggatatta tcttcagcct cgactcggtg attaccgctg tgggtctgtc
    23341 agatcacctg tttattatga tggccgccgt ggtgattgcc gtaggcgtga tgatgttcgc
    23401 cgcgcgctcg attggtgatt ttgtcgagcg ccatccttcg gtaaaaatgc tggcgctctc
    23461 tttcctgatt ctggtgggct ttaccctgat tctggaaagt ttcgacatcc acgtaccgaa
    23521 aggttacatc tacttcgcga tgttcttctc tattgcggtt gaaagcctca acctgattcg
    23581 caacaaaaag aacccgctct gatactccgt acgctctcct gcgggagggc gtactcactt
    23641 ctgttaccct cacagtttaa gattttcctg ctttcaagat atataacgtc agtttataaa
    23701 cagactatta tcataggtgg gccaaatgag gataaccgga tgaaaaaatg ggcagtaata
    23761 atttctgcag tcggactggc gtttgctgtt tccgggtgtt ccagtgatta cgtcatggcg
    23821 accaaagatg gccgtatgat tttgaccgat ggaaaacctg aaattgatga tgataccggg
    23881 ctggtgagtt atcacgatca gcaaggtaac gcgatgcaaa ttaaccgtga tgatgtttcg
    23941 caaataattg aacgttaaca aataaggtca gcatccggct ggccttaaga tttttctctt
    24001 tcccttttcc cttccctctg ccatttttat attccttatg tcgtgattat aaaaaggaaa
    24061 cggctatgca atatcaccgt ataccccaca gttcgctgga agtcagcacg ctggggcttg
    24121 gcacgatgac gtttggtgaa cagaacagcg aagccgacgc ccacgcacaa ctcgactatg
    24181 ccgtcgctca gggcattaac cttatcgacg ttgccgaaat gtacccagta cctccgcgcc
    24241 ccgaaacgca agggttaacc gaaacctacg tcggcaactg gctggcgaaa catggcagcc
    24301 gcgaaaagtt aattatcgcc tccaaagtga gcggaccgtc gcgcaataat gacaagggca
    24361 tccgcccgga tcaggcgctg gatcggaaga atatccgcga agcgctgcat gacagcctca
    24421 agcgtctgca gactgattac ctcgatcttt atcaggtgca ctggccgcag cgcccaacca
    24481 actgcttcgg caaactcggt tatagctgga cagattctgc gcctgcggtt tcgctgctgg
    24541 atacgctgga cgcactggca gagtaccaac gagcgggaaa aattcgttat atcggcgtgt
    24601 cgaacgaaac tgcatttggc gtaatgcgct acctgcatct ggcggacaaa cacgatctgc
    24661 cgcgtattgt caccattcag aacccttaca gtctgttaaa ccgcagtttt gaagtaggtc
    24721 tggcagaagt cagccagtat gaaggggtcg aactgctggc ctattcgtgc ctgggtttcg
    24781 gcacgctgac cgggaaatat ctcaatggtg caaaacccgc tggcgcacgt aatacgctct
    24841 ttagtcggtt cacccgctat agcggtgagc aaacgcaaaa agccgtcgcg gcgtatgttg
    24901 atatcgccag acgtcatggc ctggcccctg ctcagatggc gctcgcgttt gtacgccgtc
    24961 aaccgtttgt tgccagcact ctgctgggcg caaccacgat ggatcagctg aaaactaaca
    25021 tcgaaagttt gcatctggag ttaagcgaag acgtattagc tgaaattgaa gcggtgcatc
    25081 aggtttatac ttatccggca ccataagatt aacgccggtt aaaaccggcg ttaaatatta
    25141 atgacggcgc tgccagatcc acagcgccgt tattgccagc gcaaatagcg caccgaagcc
    25201 aatgccaatg ggcacgaccg ggatgcctac cattaccgcc agcgagtaaa tgcccagcat
    25261 caacaacatg gcgctgtttt cgccaaggtt ttgtactgca atcgcattcc ccgccccgac
    25321 gcttttttta ccccgctcct gtagcaacgc attgagcgga acgacaaaaa agccccccaa
    25381 cacgccaatc agcatcaaca aggcataggc tggcagctgc tcgtgttgca gggagaaaat
    25441 cagtaccacc acgccaatca aaattccggc tggcatacag cgtgacacgg tttccagcgt
    25501 aaccagcttc gctgccgcac ctgcgccaac cacgatacca atcgctacca tcgcgttgag
    25561 ataggtcggc gtagcgttat cggtaatgcc cagcgccacc ggtacccaca gcaccaacag
    25621 gaaacgcagc gtgacacccg ctccccagaa taaactggtg cccaccagcg aaaaacgcgt
    25681 ttcaccattg cgccatagcg aggtgcaggc attcaggaaa ctgcgggtca tgttgatgag
    25741 attccaggac tgccccggac gcgccgccgc cagtttggga atgtagatat tggcaacgac
    25801 cgcaccaccg taggccagtg cgcatgcggc cagggcgacc aggacatgcc agtcagccag
    25861 cacaccaccg gctacggaac cgagcaaaat cgccgctatg gtagaagctt ccattaaacc
    25921 gttagctttt actaacttac tacccgtggt taattcgccg agaataccgt atttcgccgg
    25981 tgaataggct gcagcaccaa cacccaccag cgtatagccg agaaacggat tgataccaaa
    26041 gcagatactg gctgcgccca gcagcttcag gccgttggca aacatcatca cccggccttt
    26101 ggcgaagcta tccgccacct gcccgacaaa cggcgcaaaa agaatgtaag cacctacaaa
    26161 caccatttgc aggatgggct ggctccactc cggatagaac tgcgctttca gtaacgccag
    26221 agtggcaaac agtagggcat tatcgccaaa cgcagagaga aactgcgcca cgataaccgc
    26281 tttcatcccc ttcgaccaca acgaagtgtt agtgtgcact gactcactca tcgtgttgtt
    26341 ccacttcgtc tacccagctt ttcaacgtga caaagtcagg tttgccgctg ccaagtaatg
    26401 gcatctgttt caaatagcga atatcacgcg gtacagcaag ctccggcacg ccgtgctcac
    26461 gggcatactg ttgcaactta tcgcgcgtca gttcgttatc tgtggtgaaa agcaccagtg
    26521 cctcgccttt gctggcatcg ctcttaatcg cagtggcatg gactttatct ggcgaaacac
    26581 caagtgccag ttgttccacc atttccagcg acaccatttc gcctgcaatt ttggcaaagc
    26641 gttttgcgcg gccctgaatc tgcacaaagc cctgctcgtc aaaacgcaca atatcgccag
    26701 tgtcatacca gccgcgctcc atttcgccgc gaacattctc ggcggtgggc acttccagta
    26761 cacctggctt ctccacccgc agatagccgt tcattatgtt cggccctttc agttgcaggc
    26821 gtccgccctc ttcgatacca gggaccgaca acaggcgcgc atccattcct ggtagaatac
    26881 gccctaccgt accgggtttc gccgccatcg gtacgttgat agaaacgaca ggcgcgcatt
    26941 cggtcacacc gtagccttca aggatgcgca ggccaaattt atcctgccaa agctgtttgg
    27001 tactttcttg tagtttttct gcgcctgcca ccacatagcg tagacgatag aagtcatacg
    27061 ggttggcgaa acgcgcgtag tgaccgagga aagtcgaagt gccgaacaac acggtgcaac
    27121 tgcggtcata caccaactcc ggcacaatac ggtaatgcag cgggcttgga taaagaaaca
    27181 cttctgcacc tgtgagcagt ggcgtaaaca ggcctaccgt cagcccaaag gagtgaaaca
    27241 gcggtaacgc cgacataaag cgatcgttgg tggtgaagtc ggcaatcgtt ttaatctgct
    27301 cgacattcgc cagaatgctt ttatggctat ggacgacgcc ttttggatgg ccttcagaac
    27361 cggaggtaaa aaggatcagc gcctcttctt ccggctgctg tttaacctgt gccagacgcg
    27421 gcatcagcaa atgagcgaag atccatactt tgtcggcagt ggtgacatct gcttttaaat
    27481 cttccagata gacccagcgt acctgagtaa gttgctccgg cagatgccag agtttgcctt
    27541 tatcgagaaa ctggcgggaa gtgaagatgg ttttgatttc agccgccgta atagcactgg
    27601 tcagcccttt taccccagca gtgtagttca tcattgcggg aatgcggcga cgggcgatgg
    27661 ccccaaaaat cactgccgca ctgatgcctg cattgggcag cattaagccg atgcgttcgc
    27721 cttcaacact gtatttttca aggatgcgtc caacaaacag cgttttcgta agcaatttgc
    27781 gataggagtc tggggtaaaa ttgacgtctt cgacacattt cttccctgct ccgaagcggt
    27841 acattgcact cagtaaagat tcgtacagcg tttcacgcgg gcgcaccgcc attcgcgctt
    27901 ccatcattat ttgatgcagc atttcgccag cgattttgcg acggtcacgg gcacgcggcg
    27961 catccggcat ctccacctgc gttggtggca aaatatgcag agtaatttgc gggaacaagc
    28021 gacgtttaac cagacctttc aggcggctga agtgcgtaag ttccgcccct tcaatacgca
    28081 caggaataac cgttgcacca gacttcgccg cgacaaaacc cgcgccatcg tagattttca
    28141 tcagcgagcc tgtggtggtg atgcgtcctt cagggaaaat caccactggt cggccctgtt
    28201 ccaccagacg taccagatgt ttaatcgcca taggttgcgt cgggtcgaga ggaacaaagt
    28261 cgataaatga tttcagccaa cgcatatacc actgttggct tattgaggtg taaacggcaa
    28321 acactggacg cacaggtaaa aacagtccaa gcaaaatgcc atcaataaaa gagacgtgat
    28381 taggcgtaat tagaacgcgc tcgcccttca gtgcctgggg gtcacccgta acgcgaacgc
    28441 gatacaaaac acggcacaaa tttcgaaaaa agctaaaaag cattccaact ccctttgctc
    28501 tgattcagta aaagcgaatg gagggagatt acacgagata aagaacgcga gcgacagtaa
    28561 attaggtgcg aaaaaaaacc tgcgcatccg cgcaggttgg tgcaagagac agggtacgaa
    28621 gagcgtaccg aataatctca ccaatcaata cctctgggat cttgattgtg gtctgcacga
    28681 cgactcttcg ccagcgagaa aacgcaaagg aatgaaggga aatgcaacga ggtgtgtaaa
    28741 ttgtcggtta ctgttacaga ttgatgaccg gcaaaaaaaa acctgcgcat ctgcgcaggc
    28801 tggtgtaatt catgtgctca acccgaagtt gacttcacct atcaatacct ctgggatcac
    28861 cactttagca acctgaagcc aaacgccacc agcggtcaat cgcaacagcg tttcgcaaag
    28921 tgtaaccaaa ggtttgcaat ctcttttttt gtcttactta tttgcgtttg gctcacattc
    28981 ccacgatgaa aacacgccgc cccttgaacc aacgggcgtt ttccgtaaca ctgaaacaat
    29041 gtaagcgttt acccactaag gtattttcat ggcgaccata aaggatgtag cccgactggc
    29101 aggcgtttca gtcgccaccg tttcccgcgt cattaataat tcacccaaag ccagcgaagc
    29161 ttcccggctg gctgtgcata gtgcaatgga gtctcttagc tatcacccga acgccaacgc
    29221 ccgtgcgctg gcgcagcaga ccactgaaac ggtcggtctg gtcgttggtg atgtttccga
    29281 tccgtttttc ggtgcaatgg tgaaagcggt cgaacaggtg gcttatcaca ccggtaattt
    29341 cttattgatt ggcaatggtt accacaacga acaaaaagag cgtcaggcca ttgagcaact
    29401 gatccgccat cgctgtgctg cgttggtcgt ccatgccaaa atgatcccgg atgccgattt
    29461 agcctcatta ttgaaacaaa tgcccggtat ggtgctgatc aaccgtatcc tgcctggctt
    29521 tgaaaaccgt tgtattgctc tggacgatcg ttacggtgcc tggctggcta cgcgtcattt
    29581 aattcagcaa ggtcataccc gcattggtta tctgtgctct aaccactcta tttctgacgc
    29641 cgaagatcgt ctgcaagggt attacgatgc ccttgctgaa agtggtattg cggccaatga
    29701 ccggctggtg acatttggcg aaccagacga aagcggcggc gaacaggcaa tgaccgagct
    29761 tttgggacga ggaagaaatt tcactgcggt agcctgttat aacgattcaa tggcggcggg
    29821 tgcgatgggc gttctcaatg ataatggtat tgatgtaccg ggtgagattt cgttaattgg
    29881 ctttgatgat gtgctggtgt cacgctatgt gcgtccgcgc ctgaccaccg tgcgttaccc
    29941 aatcgtgacg atggcgaccc aggctgccga actggctttg gcgctggcgg ataatcgccc
    30001 tctcccggaa atcactaatg tctttagtcc gacgctggta cgtcgtcatt cagtgtcaac
    30061 tccgtcgctg gaggcaagtc atcatgcaac cagcgactaa ccgcagttaa agcaattcca
    30121 gcgccagtaa ttcttcgatg gtctggcgac ggcgaatcaa ccgcgcctga ccattatcaa
    30181 acagaacttc tggtaacagc ggacggctat tgtagttgga tgacattgat gcgccatatg
    30241 cccctgtatc atgcagtacc agataatcac ctgccttcac ttccggcaag gcgcggggtt
    30301 caacatttcc cccttcctgc tgggtaaaga catcgcccga ttcacataac ggtccggcga
    30361 cgacggtttc caccgttggt gcgtgttcca gagaacgacc atcagctgcc agggcactga
    30421 tatggtggta actaccgtac attgccgggc gcatcagatc gttaaacccg gcatcaacca
    30481 gcacaaagtg gcggctaccc atttgtttga cgctccgcac ctgagtaatt aacacgccag
    30541 actgcgctac caggaagcga cccggttcaa tttccagttt cacagggtgg cccaaatggc
    30601 gggcgatttg ctcacgcgcg gcattccaca gaccataata atgttcggta tcaaccgcct
    30661 cttcaccctg ttgataagga atagaaagcc caccgcccgc agaaatagcc tgtaaatcct
    30721 gaccgaattc gatgacctga cgcaccatag caccacacac ctgttccaga tgggcataat
    30781 caacgccaga accaatgtgc atgtgaatgc cgaccagctg cagatgatga cgttgtatca
    30841 cgtccagtgc ggcgggcaga tcggtgtacc agataccgtg cttgctgttt tcgccaccgg
    30901 tattggtttt ttggctatgt ccgtgaccaa accccggatt aacgcgcagc catacccgat
    30961 gccctggcga aacctggccc agttggtcga acatatcaac agaacccgca ttcaccggaa
    31021 tttgcaattc actgacgcgt tcaagcgtcg cctgatcgat aacatctgcc gtaaaaacaa
    31081 tatcatcggg gtgcgtttgc ggattgtaac ccgccgccaa cgcacgctct atttcgccta
    31141 acgagacgga atccactttc acgccctgct cacgcattaa gcgcaaaata tgaatattgg
    31201 aacaggcttt ctgtgcaaag cgcaccacat caaactgttt cagcgctgca atctgccgac
    31261 gaataatttg cgcatcgtag acccacaccg ggcagccaaa ttcagcgggc aaacgcagca
    31321 gattttcggc ggtgagatcg gtatcggtgc tgaacagtga atgtggcata acaaactcca
    31381 gatgagtgct tttttatgat tacgccacat cataaaaaga ataaaaaata tcgatttatg
    31441 tcgagtctat gcaaaaatga tatggattac cggattgcga gagagcgcta atggccgccg
    31501 ttaacttacg tcatattgaa atttttcatg cggtaatgac cgccggaagc ctgactgagg
    31561 cagcacacct gctacacacc tcacagccaa ccgtcagccg cgaactggcg cgctttgaga
    31621 aggtgatcgg gctgaaattg tttgagcgcg tacgtggacg attacatcct accgtgcaag
    31681 gactgcgtct gtttgaagaa gtgcaacgat cctggtacgg actggatcgc attgtcagcg
    31741 ccgcagaaag tctgcgcgag tttcgccagg gagaactgtc tattgcctgc ctgccggtct
    31801 tttcgcaatc ttttttaccg cagctcctgc aaccctttct ggtacgttat cccgatgtca
    31861 gcttaaatat cgtgccccag gaatcaccgc tacttgaaga gtggctctcg gcccagcgtc
    31921 atgatttagg actcactgag acactccata cgcctgcggg aacagaacgt accgaattac
    31981 tctctttaga tgaattgtgt gtgttacctc cgggtcatcc gctggcggta aaaaaggtat
    32041 taacgccgga tgattttcag ggtgagaact acatcagcct ttcccgtact gacagctatc
    32101 gccagttgct ggatcagcta tttactgaac atcaggttaa acgacgcatg atcgtagaaa
    32161 cccacagcgc cgcgtcagtc tgcgcaatgg tacgggcggg ggtaggtgtt tcggtggtta
    32221 acccgctcac cgcactggat tatgcggcaa gcggtttagt ggtgcggcgg ttcagtattg
    32281 cggttccgtt caccgtcagc ctgatccgcc ccctgcaccg cccgtcatca gcgctggtgc
    32341 aggcgtttag tgagcattta caagcggggt taccgaaact ggtcacttct cttgacgcta
    32401 ttttgtcgtc agctacgaca gcataaaagc gacagcatcc tcggcatgga tcgccgcagt
    32461 atcaaacaca ggcaaaacac tgcgctcttc tggcaccagt aaaccaattt ctgtgcagcc
    32521 aaaaatgacg ccctgtgcgc cctgttctgc aaggcgagca atcacttgcg cataataagc
    32581 gcgtgacgct tcggtaaatt gccccagaca cagttcttca aaaataatct gattaatttt
    32641 cgcccgttca tccgcttcag gaataagaca attgatggaa aattgttccg tcagccgccc
    32701 gcgataaaaa tcctgttcca tggtgtaacg cgtacccagc agcgccacac gagtcattcc
    32761 ggccccggta attgcacgtc cggtggcatc cgcaatgtgt aagaaaggca gagtgcaacg
    32821 tgactcaatg gcatccgcca ctttatgcat cgtattggta cataacacaa taccttctgc
    32881 gcccgcccgc tgtaagccaa gcgccgcctc agccagaatg tccccggttt tatcccattc
    32941 cccgcgacgc tggcactctt ctatttcatg aaaatcgacg ctatgtagca gcacttgcgc
    33001 agagtgaagc ccaccaagcc gctgtttaat gccttcattt atcagacgat agtaaggaat
    33061 ggtggattcc cagctcattc ctcccagcaa accaattgtt ttcattcctc tctcctgatg
    33121 tgtgttaccg caacaaggct gaataaggaa cgagacaaac gcctcaacgg ccaggtgccc
    33181 gatctctatt aacgaaaaaa gggccggatg tacagcacat ccggcccgtg aaatcagacg
    33241 ccgatatttc tcaacttctc gcctgccatc agtttgcgtt cgatatgttc cagcgtgaca
    33301 tttttggttt ccggaatgag ccagaaagtg atgcccacaa acgcaatgtt cagcgcagtg
    33361 tagagccaga acgtaccggc agcgccaatg ctatcaagca gtgtcaggaa ggtcgcgccg
    33421 ataatcatat tcgacaccca gtttgtcgtc gtcgaacagg taataccgaa atcgcggcat
    33481 ttcagcggct gaatttcaga gcacaggatc cacaccactg gcgcggcgct catcgcataa
    33541 ccggcaatac acatcatcgt catgccaaca gagagccagg acaagccact ggaagccgta
    33601 ccgttatcaa actgcatcag gcaatagccc agcaccagag tgcctaacgc catcacgctg
    33661 aaaccaattt tcagagccgg tttacgacct gctttatcta ccgtaaagac cgcaatgaag
    33721 gtcgcgaaca taaaggtcag tcccacgacc agagtcgcaa tcatctgttg ttctgtggtc
    33781 gtaaagcccg ccattttgaa gatacgcggc gcatagtaca tgatgatgtt cataccggta
    33841 aactgctgca tcgcctgcaa caacataccg agaaacacag cacgacggac gttacggttg
    33901 atcttaaaca gtgcccaacc gccttgtttt aacttcaggc tttcacgaat ttcgttgagt
    33961 tcttctcgcg ctttttccga cgtatcgcgc agcatacgca acacttcttc cgcctcaata
    34021 tgacgcccct tttccgccag ccagcgcggg ctatttggca ggaagaccac cagaataatc
    34081 agcaggactg ctggtaaagc aagaaccccc aacattgcgc gccagttacc gctataactg
    34141 aacgctgtat cggataaaaa cgccagcacg atgccgagtg tgaccatcaa ctggtacata
    34201 ctgatcatct taccgcgaac gttttcactt gccatttcag aaagatacag aggagcggtg
    34261 taagacgcga tcccgacagc aatgcccagc accacacgag cggcgattaa catctctacg
    34321 ctggtcgcaa aagcggaccc tatagaactg agtacaaaca ggatggcccc cgccatcagg
    34381 ctgtatttac gccccaggcg gaacgacagc caaccattaa acagcgcacc aattgctgcg
    34441 ccgagcatca tgctactaac cacccattcc tgcaaacgac tggtcagcac aaagtgatcg
    34501 gtaatgaacg gcaacgctcc ggcgattacg ccgatatcaa gaccaaataa caatcctgcg
    34561 accgcagcag ctaccgaaac aaacatattc atacgccgcg tatcacgcaa agaacgtggc
    34621 gttaaagcag attccgtatt gatagtaacc attttttcct gccagcagag agtaagacat
    34681 agtgaaaaaa tacgtgaaca attcacacag gtgtcaggtc ggaaacagca taaatatgga
    34741 ttaaattgct gcgacatgcc gttatgtgat ggatattcca attttaaaat taagttgaat
    34801 tattgagatt attattaacc acctaatttt aaagcagata aaattcataa agttcattaa
    34861 ttgataattg atatggatta tttcataacc atgatatgga ttatgatgat ctagagatat
    34921 aaaaaaccct gccatgcggc agggtcataa aagtaagagg aatgaattaa cgcgccagcc
    34981 aaccgccatc cacagcaatg gtataaccat ttacataatc tgaagcgctg gaggcaagaa
    35041 acactaccgg ccccatcagg tcactcggca gtccccaacg gccagctgga atgcggtcga
    35101 gaatttccgc gctacgttgt tcatctgccc gcagttgttg ggtattgttg gtcgccatgt
    35161 aacccggagc tatcgcatta acattaatgt tgtgtttagc ccattcattc gccatcaatc
    35221 gcgtcacgcc catcacgccg ctttttgatg cggtataaga aggcacacgg atcccgccct
    35281 ggaaggagag cattgacgcg atattgataa tcttgccgcc attgccttgc gcgatgaagt
    35341 gtttcgccgc tgcctgagac atgaagaata cgctcttgat attcaggttc atgacatcgt
    35401 cccagtcctt ttcgctgaac tcgagagcat cttcgcggcg aatcaatccg gcgttattca
    35461 ccaggatatc aatatgacca aactcagcta ccgcgcgatc cagcagtcca ggaataccat
    35521 caatctttcg cagatcggcg gtcaggctta aaaaacgacg ccccagcgcc gtgacctgct
    35581 tgatggtttc agtcggttca acgatgttaa tgccaacaat gtcacagccc gcttgcgcca
    35641 gccccaacgc catcccctgg cccagcccag tatcacaacc agtgacgacc gcaactttac
    35701 cttcgagaga aaatgcactt aaaatcataa caatacctta ttcgtttatg cccacaacta
    35761 gcgcaaatct ttaacggcca catggtccat atcatcaaag acctggtttt caccgaccat
    35821 gccccagata aaggtataag ctttggttcc gacgccggaa tgaatcgacc agctcgggga
    35881 gatcaccgcc tgctcgttat gcatcacaat gtgacgcgtt tcttgcggct gccccatcat
    35941 gtggaaaacg caggcgtcat catccatatt gaaatagaaa taaacttcca tccggcgctc
    36001 gtgggtgtga cacggcatgg tgttccacaa gttacccgga gccagctccg tcagccccat
    36061 actcaattgg caggtttcca gcacatccgg gacaaaatat ttgttaatcg tgcgacggtt
    36121 actggtgagg ttatcgccta acgtgactgg agatacttcg tccggtgtga cttttttggt
    36181 aggatacgtc gtatgcgcgg gtgcgcaatt gtaataaaac ttcgccggag tgccggtatc
    36241 aatactagca aagacaactt cttttgcgcc tttacccaca tacagggcgt cgcggtgacc
    36301 gatttcatag cattgaccat cgacagtaat cgtaccggca ccgccaatat tgataacacc
    36361 taactcgcga cgttcaagga aataacttac gccgagttgt ttacccactt ccccgccaac
    36421 ggaaaccgtt ttagttaccg gcataatgcc gccaataata atacggtcaa tgtggctgta
    36481 aaccatggtg tactcatcgg cgacaaatac cttttcaacc aaaaattcat tgcgcagccc
    36541 ctgggtttcc agcgtttttg cgtgcgcact gtggatgctc tgtcttacgt ccacatcaac
    36601 ctccgataat cagtgaagcg tgaaaataga aaacgatagt gaaacactgt ttcgttttct
    36661 ctataagggg atgatatgcc catgggatcg ggttttcaat taaaaataaa acaatgtttc
    36721 atttttaagt taaggattaa aaaaagtgca gtgtcgatca cgaatctgtc atgagaatgg
    36781 aaaaatcagg gagcccggaa aatgtatgtc gacttgccag ccatcattat tcaacagatt
    36841 aatgacggca gatatttatc ggtaaggagg aaatgcagag ctattcgtca cgttcaatgg
    36901 tcaatgccac accctggccc ccgccgatac aaagcgttgc cagtcctttg cgggcattac
    36961 gtttcaccat ttcatgaacc agagaaacca ggattcggca accggaagcg cctatcgggt
    37021 gaccgagtgc gatcgcgcca ccattgacat tgacccgacg ctcatcccac tcaagcatct
    37081 tgccaaccga aagcgcctgt gcagcaaacg cttcattagc ctcgataaga tcaacatcag
    37141 ccaactgcca gcctacacgc tccaggcaac ggcgggtcgc atacaccggc gcaattccca
    37201 tcaatgccgg atctacacca acgctggcaa atgcgcggat gcgggccagc acgggtaaat
    37261 tcaacgctcg tgctttggct tcgctcatca tcattaccgc agctgcgcca tcgtttatgg
    37321 atgatgcatt acccgctgtc acagaaccga gactatcaaa tgaaggattt aaacgggcta
    37381 aaccttctgc actggcgtca gtgtgtggct gttcatcggt atcaacaacc aacgtctgac
    37441 cgttactttg ggtcattacc gggacgatct catctttaaa tcgtccggcg tcaatcgccg
    37501 ctcgcgcttt ttgttgcgag ctaagtgcgt aagcatcctg caattgacgg ctgatgccat
    37561 attcgcgagc cagattttcg gcggtgacac caatatgata atcattgaag gcatcccata
    37621 acccatcatg cacaagactg tcaaccagct ggctattgcc aagctgtgca ccggtgcggc
    37681 tatcagtcag aacatgtggt gcgcggctca tgttttcctg gccaccggcg atgacaatat
    37741 cagcctcgcc acactgtatc gcctgagtag ccagatgcag tgctttaagc ccggaaccgc
    37801 agacgtcatt aatcgtgatt gcagaaacgc tattaggcag accaccttta atagccgatt
    37861 gccttgccgg attctgccct gcccctgcag tcaacacctg accaagaatt acttcatcca
    37921 ccgcatatgc aggaacgccg gtacgttcta ttaacgcttt cacgaccaga ctaccaagtt
    37981 ccacggcgga atgacccgct aacgcaccgc gaaagcagcc gataggtgtc cgtaacgccc
    38041 cgacaatcac aacgtctttc atcacaacct cgcaccaatt aacagtgcaa tagtaaatga
    38101 ttgttaatta cagttatctt aattgtttaa aaaaagtgat ttttatcaca aaggaaatat
    38161 acctgagcag tagtcagagt cataactggc acgtaaggtt tgcaaccact accccaccaa
    38221 tagaggggta gatagggcgt taatctccca tacttaacct ggtttatggt aaattgccct
    38281 ccattttgtt taatttgtag atgatacgtt cagataatgt ctaatatttg gtctaaagaa
    38341 gaaactctgt ggagtttcgc gctctacggc acagccgttg gtgcaggcac gctcttcctt
    38401 cctattcagt taggttcggc aggggctgtg gtcctgttta ttactgctct ggtcgcctgg
    38461 cctttaacat actggccaca taaagcttta tgccagttca tcctctcatc gaaaacatca
    38521 gcaggtgaag ggataacggg cgcggtaaca cactactatg gcaagaagat tggtaatctg
    38581 attaccacgc tgtacttcat cgcctttttt gtcgtcgtgt tgatatatgc agtggcaatt
    38641 accaactcac ttacggaaca gctggcaaag catatggtta ttgatcttcg catccgtatg
    38701 ttggtgagtc tgggtgttgt attaattctg aatctcattt ttctgatggg acgtcatgcc
    38761 actattcggg taatgggatt tttggtattc ccattgattg cctatttctt atttctttcc
    38821 atttaccttg taggtagttg gcaacctgat ctattaacaa cccaggtaga gttcaatcag
    38881 aatacccttc accagatatg gatatcgatt cccgtgatgg ttttcgcctt tagccatacg
    38941 cccattattt ctacgtttgc catagacaga cgtgaaaaat atggcgaaca cgctatggat
    39001 aaatgcaaaa aaattatgaa agtcgcttat ctcatcatct gcataagtgt actgttcttt
    39061 gtctttagct gcctgctttc tattccacct tcgtatattg aagcggctaa agaagaaggg
    39121 gtcaccattt tatcggcgct ttctatgctg ccgaacgccc cagcatggtt gtcaatttcc
    39181 gggattattg tcgcagtagt tgcgatgtcg aaatcattcc tgggtacgta ctttggcgtt
    39241 attgaaggtg ccacggaggt cgtcaaaaca acactacagc aggttggtgt aaagaaaagt
    39301 cgtgcattta accgcgcact atcaattatg ttggtatcgc tgattacctt cattgtttgt
    39361 tgcattaacc cgaacgcgat ttcgatgatt tatgcgatca gcggcccgct cattgccatg
    39421 atacttttca tcatgcctac gctatcaaca tatctcatcc cggcgcttaa accctggcgc
    39481 tccatcggaa atctgattac gctgatcgtg ggtatcctgt gcgtatcggt aatgttcttt
    39541 agctaatatc cattgtgaat ggttcaaaat tgctacaatt ttgaaccatt ccagttttca
    39601 taatgtgcat ttgttattat tccgctctat cacttctctc gtcatatcct agtattatca
    39661 gttagttaaa cacacaaaga gatctgaaca tcttaaataa gactatttaa gatgcataac
    39721 ttagattcgc aagatatctt ctggtcattc aaaaacaatt tgcgatatag atattctcat
    39781 accatgagat agttatctga
//
LOCUS       DBFIFI010000020        39248 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.020, whole genome shotgun sequence.
ACCESSION   DBFIFI010000020 DBFIFI010000000
VERSION     DBFIFI010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39248
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.020"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(106..1005)
                     /gene="lysO"
                     /locus_tag="N7B39_001056"
     CDS             complement(106..1005)
                     /gene="lysO"
                     /locus_tag="N7B39_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HGU2421077.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVASSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(1463..2158)
                     /gene="aqpZ"
                     /locus_tag="N7B39_001057"
     CDS             complement(1463..2158)
                     /gene="aqpZ"
                     /locus_tag="N7B39_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HGU2421078.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRN"
     gene            2584..4242
                     /gene="ybjD"
                     /locus_tag="N7B39_001058"
     CDS             2584..4242
                     /gene="ybjD"
                     /locus_tag="N7B39_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HGU2421079.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQAHHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGHAD"
     gene            complement(4239..5195)
                     /gene="ybjX"
                     /locus_tag="N7B39_001059"
     CDS             complement(4239..5195)
                     /gene="ybjX"
                     /locus_tag="N7B39_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HGU2421080.1"
                     /translation="MNMSQLTERTFTSSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            5346..6461
                     /gene="macA"
                     /locus_tag="N7B39_001060"
     CDS             5346..6461
                     /gene="macA"
                     /locus_tag="N7B39_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HGU2421081.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            6458..8404
                     /gene="macB"
                     /locus_tag="N7B39_001061"
     CDS             6458..8404
                     /gene="macB"
                     /locus_tag="N7B39_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HGU2421082.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTASGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFMTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(8477..8701)
                     /gene="cspD"
                     /locus_tag="N7B39_001062"
     CDS             complement(8477..8701)
                     /gene="cspD"
                     /locus_tag="N7B39_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HGU2421083.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            8851..8925
                     /gene="yljB"
                     /locus_tag="N7B39_001063"
     CDS             8851..8925
                     /gene="yljB"
                     /locus_tag="N7B39_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HGU2421084.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            9024..9344
                     /gene="clpS"
                     /locus_tag="N7B39_001064"
     CDS             9024..9344
                     /gene="clpS"
                     /locus_tag="N7B39_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HGU2421085.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            9375..11651
                     /gene="clpA"
                     /locus_tag="N7B39_001065"
     CDS             9375..11651
                     /gene="clpA"
                     /locus_tag="N7B39_001065"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HGU2421086.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(11997..12084)
                     /locus_tag="N7B39_001066"
     tRNA            complement(11997..12084)
                     /locus_tag="N7B39_001066"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12048..12050),aa:Ser,seq:gga)
     gene            complement(12338..12556)
                     /gene="infA"
                     /locus_tag="N7B39_001067"
     CDS             complement(12338..12556)
                     /gene="infA"
                     /locus_tag="N7B39_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HGU2421087.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(12841..13545)
                     /gene="aat"
                     /locus_tag="N7B39_001068"
     CDS             complement(12841..13545)
                     /gene="aat"
                     /locus_tag="N7B39_001068"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HGU2421088.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRNYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(13587..15308)
                     /gene="cydC"
                     /locus_tag="N7B39_001069"
     CDS             complement(13587..15308)
                     /gene="cydC"
                     /locus_tag="N7B39_001069"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HGU2421089.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDS
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAVIL
                     RRVGLEKQLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(15309..17075)
                     /gene="cydD"
                     /locus_tag="N7B39_001070"
     CDS             complement(15309..17075)
                     /gene="cydD"
                     /locus_tag="N7B39_001070"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HGU2421090.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGETELASTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(17198..18163)
                     /gene="trxB"
                     /locus_tag="N7B39_001071"
     CDS             complement(17198..18163)
                     /gene="trxB"
                     /locus_tag="N7B39_001071"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HGU2421091.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            18708..19202
                     /gene="lrp"
                     /locus_tag="N7B39_001072"
     CDS             18708..19202
                     /gene="lrp"
                     /locus_tag="N7B39_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HGU2421092.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            19337..23365
                     /gene="ftsK"
                     /locus_tag="N7B39_001073"
     CDS             19337..23365
                     /gene="ftsK"
                     /locus_tag="N7B39_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HGU2421093.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDENIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQPQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLSPPPFD"
     gene            23520..24131
                     /gene="lolA"
                     /locus_tag="N7B39_001074"
     CDS             23520..24131
                     /gene="lolA"
                     /locus_tag="N7B39_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HGU2421094.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            24142..25485
                     /gene="rarA"
                     /locus_tag="N7B39_001075"
                     /gene_synonym="mgsA"
     CDS             24142..25485
                     /gene="rarA"
                     /locus_tag="N7B39_001075"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HGU2421095.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            25576..26868
                     /gene="serS"
                     /locus_tag="N7B39_001076"
     CDS             25576..26868
                     /gene="serS"
                     /locus_tag="N7B39_001076"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HGU2421096.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            27107..29551
                     /gene="dmsA"
                     /locus_tag="N7B39_001077"
     CDS             27107..29551
                     /gene="dmsA"
                     /locus_tag="N7B39_001077"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HGU2421097.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSGEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGIHNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            29562..30179
                     /gene="dmsB"
                     /locus_tag="N7B39_001078"
     CDS             29562..30179
                     /gene="dmsB"
                     /locus_tag="N7B39_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HGU2421098.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLMPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            30181..31044
                     /gene="dmsC"
                     /locus_tag="N7B39_001079"
     CDS             30181..31044
                     /gene="dmsC"
                     /locus_tag="N7B39_001079"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HGU2421099.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGTELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(31080..31706)
                     /gene="ycaC"
                     /locus_tag="N7B39_001080"
     CDS             complement(31080..31706)
                     /gene="ycaC"
                     /locus_tag="N7B39_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HGU2421100.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            32021..33169
                     /gene="ycaD"
                     /locus_tag="N7B39_001081"
     CDS             32021..33169
                     /gene="ycaD"
                     /locus_tag="N7B39_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2421101.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            33379..34809
                     /gene="ycaM"
                     /locus_tag="N7B39_001082"
     CDS             33379..34809
                     /gene="ycaM"
                     /locus_tag="N7B39_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HGU2421102.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYTPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(34810..34920)
                     /locus_tag="N7B39_001083"
                     /pseudo
     CDS             complement(34810..34920)
                     /locus_tag="N7B39_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(35019..35759)
                     /gene="pflA"
                     /locus_tag="N7B39_001084"
     CDS             complement(35019..35759)
                     /gene="pflA"
                     /locus_tag="N7B39_001084"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HGU2421103.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(35951..38233)
                     /gene="pflB"
                     /locus_tag="N7B39_001085"
     CDS             complement(35951..38233)
                     /gene="pflB"
                     /locus_tag="N7B39_001085"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HGU2421104.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(38288..39145)
                     /gene="focA"
                     /locus_tag="N7B39_001086"
     CDS             complement(38288..39145)
                     /gene="focA"
                     /locus_tag="N7B39_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HGU2421105.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT         9784 a         9626 c        10401 g         9437 t
ORIGIN      
        1 aaggttaaga cgcttaacgc ggggataaaa gggatttttc atgcaacttt aagggagatt
       61 gatttagcgc aattttggcg gcagggatct accgccagag aggtattacg cggagaaaaa
      121 ggcgatgagg atcggcacta acaggctaag aataaaaccg tgaacaattg ccgccgggac
      181 catatccagc ccgccagtac gttgaagaac gggcagggtg aaatccatcg acgtcgcacc
      241 gcataagccc agtgcggtag agcggctgcg gcgaatcagc ccagggatca acataattgc
      301 aatcagctca cgggccagat cattaaaaaa cgccgcgctc ccgattaccg gaccaaaaga
      361 ttcggtcaat aaaataccgg aaagagaata ccagccgaaa ccagaggcca ttgccagcgc
      421 ggtattgatg ggtagatcaa gaataaaggc gttaattaaa ccaccaatta atgaactggc
      481 aaccaccacc acggcgacaa tcattccccg gcgattaagg acaatctgct ttaaggtcat
      541 gccattattg cgcaactgaa taccgacgag gaaaagtagc agaattaacg tgtattcact
      601 ggcttcggtc gcgtgttgta agaaagccag tccactcagg ccaatggcaa aaccaatcac
      661 tactacgccg cacagtttta gcgactcaag cgccatcgca atacgcgacg ggagtttttc
      721 ttgctgatgg tggttgcgcc acggcagacc gcgctccagc cacataaggg cggcaatatt
      781 acacagtaaa ataacggtaa tactgacggc agaataatgc agaatcgcca acaggttact
      841 ggcgaggtta tcgagaaacg ccagactgat acccataaaa aagagaataa ggtaaaccat
      901 ccagcttaat agctgattaa taacttttaa cgcagcttgt tggcgaagcg gaatgaggta
      961 acccacaatc aggggaacca gaatgattaa caacccagaa aacatgaaaa accagccctt
     1021 gcaaagaaag aagtcgaaat gcgcgatgac acactactga aagcggaagg acgagtaaag
     1081 ttgcaattaa aaggaaatgt tatgcatcag agggggattt ttaattctat atgagtgttt
     1141 agcattgttg cgctcctttc atgtgcaatg ccatataaag ccactggcgc agggagcgcg
     1201 cagggggcgg ccagtcgccg ccgccccctg cacccccggg ctctggcgaa caaaatcgcc
     1261 gctgcgcggt accttcagct tatccccttc ggcaaccggg tcgggcgcga ggtaacgtcc
     1321 tgtaagacgc gccctcagcc cacatccatg tgggctgccc cggccttcgg ggaacgcttc
     1381 agcgattttg acgccaccaa tacccgtgcg gtttgtaatg tcgaagataa aacaaccagg
     1441 gcagataaaa tgccaatgct aattaattac gcttttccag cagagtccgg taaatcagac
     1501 caccgataat gccaccgaca attggcacca cccagaagaa ccacagttgt tctaatgccc
     1561 agccgccctg gaagatggca accgcggtgc tgcgcgccgg gttaacagaa gtgttagtca
     1621 ccggaatact gattaagtga atcagggtta aggccagacc aatagcgatc ggcgcaaaac
     1681 ctgccggagc gaatttgtcg gttgcaccgt ggatcactaa caggaagccc gcactcaata
     1741 ccagttcaac taccagcgcg gaaagcatgg aataaccgcc tggtgaatgc tcgccataac
     1801 cgttagaagc aaaaccgctg gctgccgcgt caaaacccgt tttaccactg gcaattaaat
     1861 acagcagcgc tgccgcaaca ataccgccga caacctgggc aattacgtag ccaacgactt
     1921 cttttgccgg aaaacgtccg ccagcccata aaccaatagt gaccgccggg ttaaaatgac
     1981 caccagaaat atgaccaaca gcaaaggcca tcgtcagaac ggtcagaccg aacgccaacg
     2041 ccacgccggc aaaaccaatg cctaattccg ggaagcctgc ggccagtaca gcactaccac
     2101 agccaccaaa aacaagccag aaagtaccaa aacattcagc tgctaatttt ctgaacatat
     2161 ccacctcaat taaaaattga ccctgtgaaa aatatggtcg ttttataggg tcgtcgtaaa
     2221 aaagtgacga cggaaataat gcgcggctat tttaaaaacg aaggcgagtc attcaccaga
     2281 taaataaatc cagtaaattt gatttagggc aacagcgggt tgccccatat agtcatttgt
     2341 ctgattgaca gtgtagtgca agcaaaagat ttaatccttt aggcgtaata aaaaataatt
     2401 tatcatgcta attatttgat tttgttgttt ttgcagactt atcagcaaga gggagtataa
     2461 cgcgattatt cgctcatttt tcagacattt gccatgctta aatgtgatgt catcacgtat
     2521 tagcaaggcc tttcccgtta tactgccagc gtaaaggata agtcacatat ttctggaggg
     2581 gatatgattc ttgagcgcgt tgaaattgtg ggttttcgcg gtatcaaccg tttgtcgttg
     2641 atgctggaac aaaacaacgt cctgattggg gagaacgcgt ggggtaaatc cagcttgctg
     2701 gacgccttaa ccctgctgct atcgccagaa tcagatctct accattttga gcgcgacgat
     2761 ttctggttcc cgccgggaga tatcaacggg cgagaacatc atctgcatat tattttgacc
     2821 ttccgcgaat cgctgccagg tcgacatcgg gttcgccgtt atcggccgct ggaagcgtgc
     2881 tggacgccat gcaccgatgg ctatcaccgt attttttatc gtctggaagg ggagagtgcg
     2941 gaagacggca gcgtgatgac actgcgcagt tttctcgata aagacggaca tccgattgat
     3001 gttgaggata ttaacgatca ggcacaccat ctggtgcgtt taatgccggt gctgcgcttg
     3061 cgtgatgccc gttttatgcg ccgtattcgt aacggcacgg tgccaaatgt ccccaatgtg
     3121 gaagtcaccg cgcgccagct cgatttcctc gcccgtgagt tgtcctcaca tccgcaaaat
     3181 ctctctgatg gacagattcg tcagggactt tccgcaatgg tgcagttgct tgagcattat
     3241 ttctctgagc agggggctgg acaggcgcga tatcgtttaa tgcggcggcg ggccagcaat
     3301 gagcaacgaa gctggcgcta tctggacatc atcaaccgga tgattgaccg acctggaggg
     3361 cgctcgtatc gggttatttt gctcggcctg tttgctactt tattgcaggc aaaaggcaca
     3421 ttgcgactgg ataaagacgc ccgtccattg ttgctgatcg aagatccaga aacgcgttta
     3481 caccccatta tgctttcagt tgcctggcat ctgttgaatc ttttgccttt acaacgtatt
     3541 gctaccacca actctggcga gctgctttcg ttaactccag tggaacatgt ttgccgcctg
     3601 gtgcgtgaat cttctcgtgt tgccgcctgg cgtctggggc caagtggctt gagcaccgaa
     3661 gacagccgcc gcatctcttt tcacattcgt tttaatcgtc cgtcatcgct gtttgctcgc
     3721 tgctggttgc tggtggaagg ggaaacggaa acctgggtta tcaatgaact ggcgcgtcag
     3781 tgcggacatc attttgatgc cgaagggatc aaggtcattg agtttgccca gtccgggcta
     3841 aagccactgg ttaaatttgc ccgccgaatg gggattgaat ggcatgtact ggtcgatggc
     3901 gatgaagcag ggaagaaata tgccgctacg gtacgcagcc tgttgaataa tgatcgggaa
     3961 gccgaacgag aacatttaac ggcgttaccg gcgctggata tggaacattt tatgtatcgc
     4021 cagggatttt ccgatgtgtt ccaccgcgtg gcgcaaatcc cggaaaatgt cccgatgaat
     4081 ctgcgcaaaa ttatctcgaa agcgatccat cgctcttcca aacccgatct tgccattgaa
     4141 gtggcaatgg aggccggacg tcgtggtgtg gactccgtac cgacgctgct gaaaaaaatg
     4201 ttctcacgcg tgctgtggct ggcgcgcggt cacgcggatt aaccgcgaaa catcgtggcc
     4261 atttgtggct gaatagcgtc gagcatctca tagcgccgac ggtattcagc ccgtttttta
     4321 ctggcgattt cggcaatctc ttttcgtgct atctgtgctg gaaggcggta atggcgttca
     4381 gcatcacata cgccgccaac cgattcccag aaagcgttgt aatcggcatg gatcttgcct
     4441 tctttatcgc gataacgcag gctgcggtaa atatgcgttt cattgctgac ggcaataatc
     4501 tgctctacct gcaaacgttg ggcaaacaga caggccgctt ccatcacgag gcgtttggga
     4561 aatagcccgt ggcaggcttt cgtcgcattc tggatttcct gatgtggaat ttcccatttt
     4621 gcgccttgca gtccaccaat aaacatcgtt cttttcccct gatattcaca cagggtaaac
     4681 gtgatttctg ccagaggaat accttcgctg ttgcggaaca ggattgtgct gtcaccttct
     4741 ttatccattg agatcatcat ggtcagctca agcgtgaact gctcgccgtt tttgccttcc
     4801 agcttcgcca gttgcagccc gggggtattc aaatataagc tgaattcttc cgccgacata
     4861 catccacgga gtaacgcata atggtaacgt aacgcctcca gcaattgctt acggctaaga
     4921 ttcgccgcaa ggtaagggcg atgcagacgc acaggcagtc gcggctggcg cgttaacaat
     4981 acattgagat taggccagtg ggaaagttcg ttcatccact caacgcttaa acgcggcata
     5041 atcaacgagc gcagcaaaaa tttctggcga aaactacggc gatgccagaa tttacccggc
     5101 cgacactgtc cacgtgccag actaagaaaa agtgacaggc tgctgagaga ttcagatgac
     5161 gtaaaggtcc gttcggttag ctgcgacata ttcatgaaat caatggttgt acatgacgtc
     5221 gatttcacca ttgcgtatct taaccaaaca tcaatagtgt gattactaac gtaaatttta
     5281 gggttttgtt gatatttcgt tgaagttaat gacccggatt ggcatatgga gtattcagaa
     5341 tatttatgaa aaagcggaaa accgtgaaga agcgttacgt tattgcgctg gtgatagtca
     5401 tcgccggact gattacgtta tggagaattc ttaacgcacc cgtgccgact tatcagacac
     5461 tgattgtgcg ccccggtgat ttacagcaaa gcgtgctggc gaccggaaag ctggacgcgc
     5521 tgcgtaaggt tgacgtgggc gcgcaggtca gcggtcagtt gaaaacgctg tcggtggcga
     5581 ttggcgataa agtaaaaaaa gaccagcttt taggggttat tgatcctgaa caggctgaaa
     5641 accagatcaa ggaggtcgaa gcaacgctga tggagctacg cgcgcagcgg cagcaggcgg
     5701 aagcggagct gaaactggcg cgggtgacgt attcccgtca gcaacgtctg gcacaaacgc
     5761 aggcggtttc actgcaggat ctcgacaccg ccgcgacgga gatggctgtg aaacaggcgc
     5821 aaattggcac cattgacgcg caaatcaagc gcaatcaggc ttctctcgat acggctaaaa
     5881 ccaatctcga ttacactcgc atcgttgccc cgatggccgg ggaagtcacg caaatcacca
     5941 ctctgcaagg ccagacggtg attgccgcac aacaagcacc gaacattctg acgctggcag
     6001 atatgagcac catgctggta aaagcgcagg tttctgaagc ggatgtaatc cacctgaagc
     6061 cggggcaaaa agcctggttt acggtacttg gcgatccact gacgcgctac gaggggcaaa
     6121 tcaaggatgt actaccgacg ccggaaaagg ttaacgacgc tattttctat tacgcccgtt
     6181 ttgaagtccc caaccccaat ggtttgctgc ggctggatat gactgcgcaa gtgcatattc
     6241 agctcaccga tgtgaaaaat gtgctgacga tccctctgtc ggcgttaggc gatccggttg
     6301 gcgataatcg ttataaagtc aaattgttgc gtaatggtga aacacgcgag cgtgaagtga
     6361 cgattggcgc acgtaacgat accgatgttg agattgtcaa agggctggaa gcgggcgatg
     6421 aagtggtgat tggtgaggcc aaaccaggag ctgcacaatg acgcctttgc tcgaattaaa
     6481 ggatattcgt cgcagctatc ctgccggtga tgagcaggtt gaggtgctga agggcatcac
     6541 cctcgatatt tatgccggtg agatggtagc gattgttggc gcttcgggtt ccggtaaatc
     6601 gaccctgatg aatattctcg gctgtctgga taaggccacc agcggcacct atcgcgtcgc
     6661 cggtcaggat gttgccacgc tggacgccga tgcgctggcg caactgcgcc gcgagcattt
     6721 cggctttatt ttccagcgtt accatttgct ttcgcattta accgccgagc agaacgttga
     6781 agtaccagcc gtctatgctg gtcttgagcg gaaacagcgg ctgcttcgcg cccaggagtt
     6841 gctgcaacga ctggggctgg aagaccgtac agagtattat ccggcacagc tttcgggtgg
     6901 tcagcaacag cgcgttagca tcgcgcgggc attgatgaac ggcggtcagg taattcttgc
     6961 cgatgaaccg accggcgcac tggacagcca ttcaggcgaa gaggtgatgg cgatcctgca
     7021 tcagctgcgc gatcgcgggc atacggtgat tatcgtcact cacgatccgc aggtcgctgc
     7081 tcaggccgag cgggtgatcg aaattcgcga cggcgaaatt gtgcgcaatc ctcccgccat
     7141 tgaaaaagtg aatgttgctg gcgggacgga acctgttgtc aacacggcgt ctggctggcg
     7201 gcagtttgtc agcggtttta atgaggcgct gacgatggca tggcgggcgc tggcagcgaa
     7261 taaaatgcgt actttactga ccatgctggg gattataatc ggtattgcgt cggtggtttc
     7321 cattgtcgtg gtgggcgacg ccgccaaaca aatggtgctg gcggatattc gttctattgg
     7381 tacgaatact attgatgtct atcccgggaa agattttggc gatgacgatc cgcaatatca
     7441 acaggcgctg aagtacgatg acttaatcgc catccaaaaa caaccgtggg tcgcttcagc
     7501 cacacctgcc gtctcgcaaa acctgcgcct gcgttataac aatgttgatg ttgctgccag
     7561 tgccaatggc gtgagcggcg attattttaa tgtctatggc atgaccttca gtgaaggaaa
     7621 cacctttaac caggaacagc tgaacggtcg tgcgcaggtc gtggttctcg acagtaatac
     7681 tcgccgccag cttttccccc ataaagcaga tgtggttggc gaggtgattc tggtcggcaa
     7741 tatgcccgcc agagtcattg gtgtggcgga agaaaaacag tcgatgtttg gtagcagtaa
     7801 agtgctgcgt gtctggctac cttacagcac gatgtccggg cgagttatgg gccagtcgtg
     7861 gctgaactcc attactgtca gggtgaaaga aggatttgac agcgccgagg cggaacagca
     7921 actcacgcgt ttactttcac tgcgccacgg taagaaggat ttctttacct ggaacatgga
     7981 cggcgtcttg aaaaccgttg aaaagaccac acgtacttta caactgttta tgacgctggt
     8041 ggcggtgatt tcgctggtag tgggcggtat tggtgtaatg aatattatgc tggtgtcagt
     8101 gaccgagcgg acgcgggaaa ttggcattcg catggctgta ggtgcgcgag caagcgatgt
     8161 tttgcaacag ttcctgatcg aagccgtact ggtttgcctg gtcggtggcg cgttgggaat
     8221 aacactgtca ctgttaattg ctttcacctt gcagcttttc ttacccggct gggagattgg
     8281 tttttcaccg ttggcactgc tgctggcgtt tctctgctcg acggtcaccg ggattttatt
     8341 tggctggtta cccgcacgaa atgcggcacg actggatcca gtagatgctc tggcacgaga
     8401 gtaatttttg agataaaaat gccagccgat cgggctggca ttttgccttt aggatgtaca
     8461 caatgagaca gaagagctat gcgactgccg cttctacttc gacgggcaca ataacactgg
     8521 cgtgattgcc ttttggcccc tggtggacat caaactgaac ggattgtcca gcttttagcg
     8581 ttctgtaacc atccatctga atggtggaat aatgagcgaa aatatcttcg ccgccgcctt
     8641 cagggcagat gaaaccaaac cctttggcat tgttgaacca cttaacagta cccttttcca
     8701 tgcttcgaca tccttcgcaa atcttataca agtaagatgg aataaaccgg ggtcagagag
     8761 ggggctgttc aaaacctcgc caactctaga aatacaattt agagaattag ggcgagccgt
     8821 caagcatttg acaggggaca gggggcaggt atgaatcaaa aatttgaagc agttaacgct
     8881 attgacagga atgtgacaga tgtcgctgat gccaacgata gatgatagtt atctatcatg
     8941 tggagtagat tggtcaggca aataagctct tgtcagcggc agggcgttct gccgataacc
     9001 gtaaccgaag atgataactg acaatgggta aaacgaacga ctggctggac tttgatcaac
     9061 tggcggaaga aaaagttcgc gacgcgctaa aaccgccatc tatgtataaa gtgatattag
     9121 tcaatgatga ttacactccg atggagtttg ttattgacgt gttacaaaaa ttcttttctt
     9181 atgatgtaga acgtgcaacg caattgatgc tcgctgttca ctaccagggg aaggccattt
     9241 gcggagtctt taccgccgag gttgcagaaa ccaaagtggc gatggtgaac aagtacgcga
     9301 gggagaatga gcatccattg ctgtgtacgc tagaaaaagc ctgaatgcag gcataaaaat
     9361 tgggggaggt gcctatgctc aatcaagaac tggaactcag tttaaatatg gctttcgcca
     9421 gagcgcgcga gcaccgtcat gagtttatga ccgtcgagca cttgttactg gcgctgctca
     9481 gtaacccatc tgcccgggag gcgctggaag cgtgttctgt ggatttggtt gcgctccgtc
     9541 aggaactgga agcctttatt gaacaaacca cacccgttct gcctgccagt gaagaggagc
     9601 gcgacacaca gccgacgctg agttttcagc gtgtactgca acgtgcggtc ttccatgtcc
     9661 agtcctccgg tcgcaatgag gttaccggtg caaacgttct ggtcgctatc tttagcgaac
     9721 aggagtcgca ggcggcatat ctgttgcgta aacatgaagt cagccgtctc gatgtggtga
     9781 actttatctc tcatggcacg cgtaaagacg agccgacaca gtcttctgat cctggcagcc
     9841 agccaaacag cgaagaacaa gctggtgggg aggaacgtat ggagaatttc acgacgaacc
     9901 tgaatcagct tgcgcgcgtg ggcggaatcg acccactgat tggtcgtgag aaggagctgg
     9961 agcgtgctat tcaggttctc tgccgtcgcc gtaaaaacaa cccgctgcta gtgggggaat
    10021 ctggtgtcgg taaaaccgcg attgcagaag gtcttgcctg gcgaattgtt cagggcgatg
    10081 tgccggaagt gatggctgac tgtacgattt actctctcga tatcggttct ctgttagcgg
    10141 gcacaaaata tcgcggcgac tttgaaaaac gttttaaagc gttgctcaag cagctggagc
    10201 aggacactaa cagcatcctg tttattgatg agatccacac cattatcggt gcgggtgcag
    10261 cgtctggtgg tcaggttgat gcggctaacc taatcaaacc gttgctctcc agcggtaaaa
    10321 ttcgcgtaat tggttcgaca acctatcagg agttcagcaa cattttcgag aaagaccgtg
    10381 ctctggcgcg tcgcttccag aaaattgata ttactgaacc gtcgatcgaa gaaactgttc
    10441 aaatcatcaa tggcctgaaa ccgaagtatg aagcacacca cgacgtgcgt tataccgcaa
    10501 aagcggtgcg tgcggcggta gagctggcgg tgaaatacat taacgatcgt catctgccgg
    10561 ataaagccat tgatgttatc gacgaagcgg gcgctcgcgc acgtctgatg ccggtaagca
    10621 aacgcaagaa aaccgttaat gtggcggata ttgagtccgt ggtggcccgt attgcacgca
    10681 ttccagagaa gagtgtttct cagagtgacc gcgataccct gaaaaacctc ggcgatcgcc
    10741 tgaaaatgct ggtcttcggt caggataaag ccattgaggc gctgactgaa gccattaaga
    10801 tggcgcgtgc aggtttaggt cacgaacata aaccggtcgg ttcgttcctg tttgccggcc
    10861 ctaccggggt cgggaaaaca gaggtgacgg tacagctttc gaaagctttg ggcattgagc
    10921 ttctgcgctt tgatatgtcc gagtatatgg aacgccatac cgtcagccgt cttattggtg
    10981 cgcctccggg atacgttggt tttgatcagg gcggtttgct gactgatgcg gtcatcaagc
    11041 atccacatgc ggtgctgctg ctggacgaaa tcgagaaagc gcacccggac gtgttcaata
    11101 ttctgttgca ggtgatggac aacggtacgc tgaccgataa caacggacgc aaagcggact
    11161 tccgtaacgt ggtactggtg atgaccacta acgccggggt acgggaaact gagcgcaaat
    11221 ccattggtct tatccaccag gataacagca ccgatgcgat ggaagagatc aagaagatct
    11281 ttacaccgga attccgtaac cgtctcgaca acattatctg gttcgatcat ctgtcaactg
    11341 acgtgatcca tcaggtggtg gataaattca tcgtcgagtt gcaggttcag ctggatcaga
    11401 aaggtgtttc tctggaagtg agccaggaag cgcgtaactg gctggccgag aaaggttacg
    11461 accgggcaat gggcgcacgt ccgatggcgc gtgtcatcca ggacaacctg aaaaaaccgc
    11521 tcgccaacga actgctgttt ggttcgctgg tggacggcgg tcaggtcacc gtcgcgctgg
    11581 ataaagagaa aaatgagctg acttacggat tccagagtgc acaaaagcac aaggcggaag
    11641 cagcgcatta atctgattgt caggtaggtt ggtgaagtcc gtaatctcga aagaggttac
    11701 ggacttttta tttatggggt ggaacggtga aaaccctatt tttggaggtg aaggtaattt
    11761 gttgataatt agtgctgctg gaaaaggtaa ggataaaaaa gggtgacagc aggaaaatga
    11821 gatggttttg ctttattaac aacgggctaa acgtgtagta tttgagttca ctgccgtaca
    11881 ggcagcttag aaattcactg gtaacatact ccacccgccc accatgttca ctgccgtaca
    11941 ggcagataaa atgcgaaaaa aaagcccgta ctttcgtacg agctcttctt taaatatggc
    12001 ggtgaggggg ggattcgaac ccccgatacg ttgccgtata cacactttcc aggcgtgctc
    12061 cttcagccac tcggacacct caccaaattg ttttgctgcc aaacctcatg ggtggcaacg
    12121 gggcgctact atagggagtt ggagtaaaac ggtcaagaag aattttaatg ataattattg
    12181 tttgctcata ctgtaaacaa cttgtgcagt atatctacat cgagacaagt tatggactta
    12241 tacttccaaa gtacttcatg catatcacaa aataaaaagg ccggttaaac cgacctttta
    12301 ctcgttcttt ctcttcgccc atcaggcggt aaaacaatca gcgactacgg aagacaatgc
    12361 ggcctttgct caggtcgtac ggggtcagtt caacagtcac tttgtcgccc gtcaggatgc
    12421 ggatgtagtt tttgcgcatt ttaccggaga tgtgtgcagt aaccacgtga ccgttttcta
    12481 actctacgcg gaacatggta ttaggcaacg tttcaagaac ggtaccttgc atttcaatat
    12541 tgtcttcttt ggccatctaa tcctctgggg tatcactacc gtaatttgaa ccggcaagat
    12601 aatgccgaag ttctgtaaat aagtaaagat ttgcgcgcta aatcgcaaca aacaggttcg
    12661 gcacattact ccgaaaacac acggctaagc cgcaccaaaa gcgcaacgta taagggagcg
    12721 gtgagataaa cgatgggcgt tacctgacgc gaaaaattcc ttatcggcag cggggtaatg
    12781 agcgtaacca actctgcgac cgcaattata acactctggg gagaaatgtg ccgaaaacat
    12841 tcattcttgt ggtgaaaaca agcatcgtgg tacccagaaa ttattcggca atcgtccgag
    12901 gcgcatttga ttgagataat taaggtaatt ccggcgggga atttcgcagg caccaagcga
    12961 tgctgtgtga tcgttaagga cctggcagtc gataagctta ccgccatgac cgataaattc
    13021 ctcacagaat accagaagcg ccgttttaga cgcattttcc atccggctga acatggactc
    13081 gccacaaaat agcgttccct gggccacgcc gtacataccg ccgacaagct catcttcacg
    13141 ccagacttca atggagtggg catgaccgag ttcgtgaagg cgatggtagg cttcgaccac
    13201 gccacgcgtg atccaggttc cttcttcgcg atcgctggca cagccttcaa tgacctgacc
    13261 aaaagcgtaa ttcatcgtga cacgataggg cgagcgttta tgaaatcgct tcatactacg
    13321 gctgatatgc agtgattctg gccatagcac cgcgcgggga tcgggcgacc accagaggat
    13381 ggggtcgcct ggagaaaacc acggaaaaat accacgctgg taagccatta acaggcgcgc
    13441 agggctaaga tcgcccccaa gtgccagcag gccgttaggc tcacgtaatg cgccttccgg
    13501 ggaagggaag gctattgaat ggcgagaaag ctgaaccagg cgcatgaccg caaaactcca
    13561 cgcaagtcgg atcgttcaat aatagcttac aaaccctgct tgaactggta ataacgcccc
    13621 tgtctggcaa gcagttctgc gtgagtacct tgctcaataa tttgcccgtt gtccatcact
    13681 attatttgtt ggaaacgaga gagtccgcga agtcgatggg tgaccattaa caccgttttc
    13741 tcacgcatca tttctgcaag caattcaagg atctggcttt cggttgtggc atctaagcct
    13801 tcggtaggtt catccagcaa caccagtggc gcatcatgta acagcgcacg ggcgatagcc
    13861 agacggcgca gttcaccacc ggagagctgg cgtccgcctt cacctaacca actgttgaga
    13921 cctgcatcct cgagctgctt ctccagacca acgcgacgta agatcaccgc cagagcctca
    13981 tcactactgc caggcgaggc gagtaacaga ttatcacgca gcgtggcgct aaacagatgc
    14041 actcgctgag gaacaacgct gatggtctgt cgtagagccg cttcattcag gctggctatg
    14101 gggctatcgt taagcaaaat ctcgccctgt tgcgggtccc atgcgcgggt cagcagttgt
    14161 aacagtgttg atttgccgca tccggttcgc ccgagaatcg ctatatgttc cccggcgttt
    14221 acctgaagag aaatcccttt aagtgcctgt tgggattgct ccggataagt gaactgaaca
    14281 tcccgtaatg tcagcgaaac gcgatcggca acacgagttt gggtatcagg gaaggtgacc
    14341 tccggttttt gatccgttaa gtcagagata cgtacggcag aggcaatgac ttgccccaga
    14401 tgctgaaatg cacccgttac tggtgccagt gcttcaaacg cggctaacgc gcaaaagaca
    14461 aacagggcaa ttaacgcgcc gggttgagca ttgccgccaa cgccgccaga cgccatccac
    14521 agcatcagga tcaccgctaa cgcgccaatg agcagcatta tcgcttgcga caatgcggtc
    14581 agttcagatt gacggcgttg cgcttccagc cattgaattt ctgtattctc tagttgcgtg
    14641 cgataacgat cgctggcacc aaaaatggtc agctcagctt gcccttgcag ccaggccgtc
    14701 agttgttggc gatactgtcc gcgaagatga gtcagatttt gcccggtgct ttttcccgca
    14761 cgataaaaca gcggtggcat caggaaaagc gtcagtaaca taatgccgcc cagcgtaaag
    14821 gcgagggtgg aatcaaggaa acttaacccg attgtcacca ccataatcac cacaaaagcg
    14881 cccaccagcg gcgagataac gcgcaggtaa agatgatcga gcgtatcaac atccgccacc
    14941 acgcggttga gcaattcgcc ctgacgatag cgcgccagtc cggcagggga gaggggcaac
    15001 aatttgctga aggtgtaaat gcgcagatgc tgcaacacgc ggaaagtcgc gtcgtgactt
    15061 accagacgtt caaaataacg cccggcagta cgggtgattg ctgcaccacg cacgcccgca
    15121 gcgggtagca tatagttgaa gctgtacagt ccggcaaccc ccgcaaccgc tgaggccgag
    15181 aggaaccagc cggaaagtgt caacagaccg atactggcga gcagcgtcac aattgccagc
    15241 acaataccaa gacttaacat ccatttatga cgtttataca gtgccagata gggtagcaaa
    15301 gcgcgcattt aaatctcctc ctgacgatgg gccagtaatg tggcgaatgg gccaccagcc
    15361 acacttaatt ccgcgtaacg tccttgctca ataatccggc catcctgcat aacccaaatg
    15421 acatcccagt cagcaagatc ttctaactgg tgggtgacca ttaacgttgt ctggcgcaga
    15481 gaggcggcat tcagcgcctc cattacgcgc tgttcactgt gagcatcaag gctggcagcg
    15541 ggttcatcca acagtaatag cgaacaggga tttagtaacg cacgggccac cgccacgcgc
    15601 tgcgcctgcc ccacggaaag gcgggcagcc tggtcgccaa caggcgtatc aacgccttgt
    15661 ggcaggagcg gtagaaactc gctgacccag gcgttatcca gcgctgcttg taattcttgt
    15721 tcgctggcat caggtcgcgc cagtagtacg ttatcccgta atgttgctgc cggtaattgt
    15781 gggttttgac caacccagga gagatgttta cgccatgatt ctggtgataa atcgcgtaat
    15841 tctatcccgt tgattcgtaa tgatccctga tatgagagaa aaccagaaag cgcgttcagc
    15901 agtgagcttt tacctgaacc gctgcgacca accaacactg cacgttggcc tgctggcaaa
    15961 gtaaagttca gcggtccggc cagcgtttta ccttccggcg acgtgataaa cagatcctcg
    16021 gcctcaatgg tcagcggatc ggttgatgct aattccgtct caccgcgctg cggatgggcg
    16081 agcggggttt ccataaacgt tttcagactg tcagctgcac caacagcctg ggctttagca
    16141 tgataaaacg taccgagatc gcgtaatggc tggaaaaact ctggcgcaag gatcagggcc
    16201 agaaaacccg cagccagcgt cacgccagta tcgtagtgac caaaatccag ctcgccgaga
    16261 taggaaaaac caaagtagac cgccaccaga gcaattgaca gcgaggtaaa aaattcgaga
    16321 atgccggagg ataaaaacgc cagccgtagc acttccattg tccgttggcg gaaatcttcc
    16381 gaagcagaac gaatactttc aatttcagct tcaccacgac caaaaatacg caatgtttcc
    16441 atgccgcgca ggcgatcgag gaaatgccca cttaagcgag caagagcgag aaagttacgt
    16501 cggttagcat cggcagcccc catcccaacc agcgccataa acaacggaat taacggtgca
    16561 gtgcccagca gaatgagcgc cgcagcccag ttagagggga agatcgccac cacaatcagc
    16621 aacggcaccg acactgccag cgccatttgc ggcaggtagc gtgcatagta atcatgcata
    16681 tcgtcaattt gctcgagtac cagcgtcgcc cagctccccg caggtttacc ctgaatccac
    16741 gctggccctg cttgttgcag acggtcgaga acctgacggc ggatggcaaa gcggatatgc
    16801 tgcccggcgt gataacccac ccgttcgcgt aaccagacca cccatgcgcg cagtacaaag
    16861 gtcagaacca gtaacgtaaa gggaagcagc agggcttcac ggggaatatt ctccataatc
    16921 atatgttgca gaattcgcgc catgaaccat gcctgggcaa tgatcaatat gccgctcaca
    16981 aagcccagca gacgagaaat attcagccaa cgttgggaga tgacgctttg ctgttttaac
    17041 cagcgggtta actctttttg acgagattta ttcattgcac gcttagcagg tgagttatca
    17101 gaattatttg cagagcaatg ttacaacggg gaaaaaataa aggcgaccca tagtcgcatg
    17161 gtgtcgcctt ctttactttt gttactgatt tgtaaaatta ttttgcgtca gctaaaccat
    17221 cgaggtagcg ttccgcatca agtgctgcca tgcagcctgt accagcagat gtaatggcct
    17281 ggcgataaat gtgatccatc acgtcgcctg cggcaaagac gccaggaatg ctggtctggg
    17341 tggcattacc atgaataccc gactgcactt tgatgtagcc gttttccagt tccagctgcc
    17401 cttcgaaaat ggcagtattc gggctgtgac cgatagcaac aaacagaccg gcaacgtcga
    17461 gtgactcgat gttatcgctg ttttgcgtat cgcgcagacg aacgccagtg acacccattt
    17521 gatcgccggt cacttcttcc agcgtacggt tggtgtgcag aatgatgttg ccgttctcca
    17581 ctttatccat cagacgctta atgaggattt tttccgcgcg gaaaccgtca cggcggtgaa
    17641 tcagatgcac ttccgaagcg atgttagaca gatacagcgc ctcttcaacc gcggtattgc
    17701 cgccgccgat gaccgcaact ttctggttgc gatagaagaa accgtcgcag gttgcacaag
    17761 cagaaactcc acggccttta aaggcttctt cagagggcag gccgagatag cgtgcagaag
    17821 ctccggtggc aataatcagc gcgtcgcaag tgtattcgcc gttatcgcca ttcagacgga
    17881 acggacggtt ttgcagatcc accttgttga tatgatcaaa aatgatctca gtttcaaact
    17941 tggtggcatg ttcgtgcatg cgctccatta ataacggacc ggtcagatcg tttggatcgc
    18001 caggccagtt ttccacttcc gtggtggtgg tcagttggcc gcctttttcc atgccggtaa
    18061 tcagcacagg ttgcaggttg gcgcgcgccg catagacagc agcggtgtat cccgccgggc
    18121 ctgaacccag gataagcagt ttactgtgtt tggtcgtgcc catgagatcc ccatagttgt
    18181 tggcagacaa tgggcaggat tgtagggaat ttacagacgt aaaaaaagag tatgacgatt
    18241 ttgttaacaa tttgtgcaat cggcagcatc gataagcagg tcaaattctc ccgtcattat
    18301 cacctctgct acttaaattt cccgctttat aagccgatta aatgatgaat aaacgcccct
    18361 gttaatgaat atctggcatg ttgtactaaa aatcgatgtt ttgctttgac aatcccctgg
    18421 tgttttgcga aaacattcga ggaagaaaaa aaacagtatt cttatatgcg cataaccatg
    18481 catgtaaata ccatgtttac cgtgctagtg aaatctacgt atggcgtgga cagacgccat
    18541 tcgtgatgtc gatagctgcc acaaggcaac ggtcttctca ccgtagaccc aggcattgcg
    18601 cgccgtgaat cttcatgatt tcggtctatc gtgacgggta gcgactctga acagtgatgt
    18661 ttcagggtca gacaggagta gggaaggaat acagagagac aataataatg gtagatagca
    18721 agaagcgccc tggcaaagat ctcgaccgta tcgatcgtaa cattcttaat gagttgcaaa
    18781 aggatgggcg tatttctaac gtcgagcttt ctaaacgtgt gggactttcc ccaacgccgt
    18841 gccttgagcg tgtgcgtcgg ctggaaagac aagggtttat tcagggctat acggcgctgc
    18901 ttaaccccca ttatctggat gcatcacttc tggtattcgt tgagattact ctgaatcgtg
    18961 gcgcaccgga tgtgtttgaa caattcaata ccgctgtaca aaaacttgaa gaaattcagg
    19021 agtgtcattt agtatctggt gatttcgact acctgttgaa aacacgcgtg ccggatatgt
    19081 cagcctaccg taagttgctg ggggaaaccc tgctgcgtct gcctggcgtc aatgacacac
    19141 ggacatacgt tgttatggaa gaagtcaagc agagtaatcg tctggttatt aagacgcgct
    19201 aacacggaac aggtgcaaaa tcggcgtatt ttgattacac tcctgttaat ccatacagca
    19261 acagtactgg ggtaacctgg tactgttgtc cgttttagca tcgggcagga aaagcctgta
    19321 acctggagag cctttcttga gccaggaata cactgaagac aaagaagtca cattgacaaa
    19381 gttaagtagc ggccgccgcc ttctggaggc gttgctgatc cttattgtcc tgtttgccgt
    19441 ctggttgatg gctgccttac taagctttaa cccttcggac cccagctggt cgcaaacggc
    19501 ctggcatgaa cctatccata atttaggtgg gatgcccggt gcgtggttgg cagatacgct
    19561 gttctttatt tttggcgtga tggcttacac cattcccgtc attattgtcg gcggttgttg
    19621 gtttgcctgg cgtcatcagt ccagcgacga aaacattgat tattttgccg tttcgctacg
    19681 catcattggc gttttggcgc tcatccttac ctcctgtggt ctggcggcaa tcaacgctga
    19741 cgatatctgg tattttgcct ccggtggcgt cattggcagc ttactaagca ctacgctaca
    19801 accactgcta cacagtagcg ggggaactat tgcgctgctc tgcgtttggg cagcgggcct
    19861 gacgttgttc accggttggt catgggtgac cattgctgaa aaactcggcg gctggatttt
    19921 aaacattctc accttcgcca gtaatcgtac ccgtcgcgat gatacctggg tcgatgaaga
    19981 tgagtatgaa gacgacgaag agtatgaaga tgaaaaccac ggcaaacagc atgaatcacg
    20041 ccgtgcccgt attcttcgcg gcgcgctagc gcgtcgtaaa cggttggcgg aaaaattcat
    20101 taatccgatg gggcggcaaa cagacgctgc gttgttctcc ggtaagcgga tggatgatga
    20161 cgaagagatt acctacactg cacgcggtgt ggctgccgac ccggacgacg tcctattttc
    20221 gggcaatcgt gcaacgcagc cagaatatga cgaatacgat ccattattaa acggtgcgcc
    20281 aattaccgaa cctgtcgctg tagcagctgc tgctaccacg gcgacacaaa gctgggctgc
    20341 gccggttgaa cctgtgactc agacgccgcc tgttgcctct gttgatgttc caccttcgca
    20401 acctacagta gcctggcagc ctgtaccggg tccacaaacg ggagagccgg ttattgctcc
    20461 tgcaccggaa ggttacccac agcagtcaca atatgcgcag cctgcagtgc aatataatga
    20521 gccgctgcaa caaccagtac agccgcagca gccgtattat gcacctgcag ctgaacaacc
    20581 tgcgcaacag ccgtattatg cccctgcgcc agaacaaccg gtggcaggta acgcctggca
    20641 agccgaagag cagcaatcca cttttgctcc acagtctaca taccagactg agcaaactta
    20701 tcagcagcca gccgctcagg agccgttgta ccaacagccg caacccgttg aacagcagcc
    20761 tgttgtggag cctgaacccg ttgtagaaga gacaaaaccc gcgcgtccgc cgctttacta
    20821 ctttgaagaa gttgaagaga agcgagcccg tgaacgtgaa caacttgcgg cctggtatca
    20881 accgattcca gaaccggtta aagaaccaga accgatcaaa tcttcgctga aagcaccttc
    20941 tgttgcagca gtacctccag tagaagccgc tgccgctgtt tctccgctgg catctggcgt
    21001 gaaaaaagcg acactggcga cgggggctgc cgcaaccgtt gccgcgccag tcttcagtct
    21061 ggcaaatagc ggtggaccgc gtccgcaggt caaagagggg attggcccgc agttgccacg
    21121 accgaaacgt atccgcgtgc caactcgtcg tgaattggcg tcttacggta ttaagctgcc
    21181 ctcacagcgt gcggcggaag aaaaagcccg tgaagcccag cgcaatcagt acgattctgg
    21241 cgatcagtac aacgatgatg aaatcgatgc gatgcagcag gatgaactgg cacgtcagtt
    21301 cgcccagaca cagcagcaac gctatggcga acagtatcaa catgatgtgc ccgtaaatgc
    21361 agaagatgca gatgctgcgg cagaggctga actggctcgt cagtttgcgc aaactcaaca
    21421 acaacgttat tccggcgaac aaccggctgg ggcgaatccg ttctcgctgg atgattttga
    21481 attttcgcca atgaaagcgt tgctggatga tggtccacac gaaccgctgt ttacgccaat
    21541 tgttgaacct gtacagcagc cgcaacaacc ggttgcaccg cagcagcagt atcagcagcc
    21601 acaacagccg gttgcgccac agccgcagta tcagcagcca caacagcagg ttgcgccgca
    21661 gccacaatat cagcagccgc aacaaccggt tgcgccacag cagcaatatc agcagccgca
    21721 acaaccagtt gcgccgcagc cgcagtatca acagccgcaa caaccggttg caccgcagcc
    21781 gcagtatcag cagccacaac agccagttgc gccacagccg caggatactc tgcttcatcc
    21841 gctgttgatg cgtaatggcg acagccgtcc gttgcataaa ccgacgacgc cgctgccttc
    21901 tctggatttg ctgacaccgc cgccgagcga agtggagccg gtagatacct ttgcgcttga
    21961 acaaatggct cgcctggtgg aagcgcgtct ggctgatttc cgtattaaag ccgatgtcgt
    22021 caattactct ccggggccgg ttatcactcg ctttgaactt aacctggcac cgggcgtaaa
    22081 agcggcgcgc atttctaact tgtcacggga ccttgcccgt tcactttcga cggtggcggt
    22141 gcgtgtcgtt gaagttattc ctggcaaacc ctatgtaggt ctggagttac cgaataaaaa
    22201 acgacaaacc gtttatctgc gcgaagtatt ggataacgcc aaattccgcg ataatccatc
    22261 gccattaacc gtggtgctgg gtaaagatat cgccggtgag ccggtggttg ccgatctggc
    22321 gaaaatgccg cacttgttag ttgcggggac taccggttcc ggtaaatctg tcggtgtgaa
    22381 cgcgatgatc ctgagcatgc tttataaagc acagccagaa gatgtgcgtt tcatcatgat
    22441 cgacccgaaa atgctggagc tttcggtgta tgaaggcatt ccgcacctgt taacggaagt
    22501 cgttactgat atgaaagatg ccgccaacgc gctgcgctgg tgtgttaacg agatggagcg
    22561 tcgttataaa ctgatgtctg cgctgggtgt gcgtaatctg gcgggttata acgaaaaaat
    22621 tgctgaagcc gatcgcatga tgcgtccgat tccagacccg tactggaagc cgggtgacag
    22681 tatggatgcc cagcatccgg tgctgaaaaa agaaccatac attgtggtgt tggtggacga
    22741 atttgccgac ctgatgatga cagtaggtaa aaaagtggaa gagctgatag cacgtctggc
    22801 gcaaaaagcc cgtgccgcgg gtatccacct cgtactggca actcagcgtc catcggttga
    22861 tgttattact ggtctgatta aagcgaatat tccgacccgt atcgccttta ccgtatccag
    22921 taagattgac tcacgtacca ttcttgatca ggctggcgcg gaatcactgc tgggtatggg
    22981 ggatatgctc tactctgggc cgaactccac gttgccggta cgtgtccatg gtgcttttgt
    23041 tcgcgatcag gaagttcatg ccgtggtgca ggactggaaa gcgcgtggtc gcccacagta
    23101 tgttgatggc atcacctccg acagcgaaag cgaaggtggt gcgggtggtt tcgatggcgc
    23161 tgaagaactg gatccgttgt tcgatcaggc ggtgcagttt gtcactgaaa aacgcaaagc
    23221 gtcaatttct ggcgtacaac gtcagttccg cattggttat aaccgtgcag cgcgtattat
    23281 cgaacagatg gaagcgcagg ggattgtcag cgaacagggg cacaacggta atcgtgaagt
    23341 gctgtcccca ccgccgtttg actaattaat gcattgccgg ataaggcgcg gtagcgtcgc
    23401 atccggcact ctatcaactg aaaattcagt attttcttct ttcctcaagc tgattattag
    23461 cctggaatag agagtagagg gaactcccga tcgggagtga cgtaatttga ggaataatga
    23521 tgaaaaaaat tgccatcacc tgtgcattac tctcaagctt agtagcaagc agcgtttggg
    23581 ctgatgccgc aagcgatctg aaaagccgcc tggataaagt cagcagcttc cacgccagct
    23641 tcacacaaaa agtgactgac ggtagcggcg cggcggtgca ggaaggtcag ggcgatctgt
    23701 gggtgaaacg tccaaactta ttcaactggc atatgacaca acctgatgaa agcattctgg
    23761 tttctgacgg taaaacactg tggttctata acccgttcgt tgagcaagct acggcaacct
    23821 ggctgaaaga tgccaccggt aatacgccgt ttatgctgat tgcccgcaac cagtccagcg
    23881 actggcagca gtacaatatc aaacagaatg gcgatgactt tgtcctgacg ccgaaagcca
    23941 gcaatggcaa tctgaagcag ttcaccatta acgtgggacg tgatggcaca atccatcagt
    24001 ttagcgcggt ggagcaggac gatcagcgca gcagttatca actgaaatcc cagcaaaatg
    24061 gggctgtgga tgcagcgaaa tttaccttca ccccgccgca aggcgtcacg gtagatgatc
    24121 aacgtaagta gaggcacctg agtgagcaat ctgtcgctcg atttttcgga taatactttt
    24181 caacctctgg ccgcgcgtat gcggccagaa aatttagcac agtatatcgg tcagcaacat
    24241 ttgctggctg cggggaagcc gttgccgcgc gctatcgaag ccgggcattt acattctatg
    24301 atcctctggg ggccaccggg taccggcaaa acaaccctcg ctgaagtgat tgcccgctat
    24361 gcgaacgctg atgtggaacg tatttctgcc gtcacctctg gtgtgaaaga gattcgcgag
    24421 gcgatcgagc gcgcccggca aaaccgcaat gcaggtcgcc gcactattct ttttgttgac
    24481 gaagttcacc gtttcaacaa aagccagcag gatgcatttc tgccacatat tgaagacggc
    24541 accatcactt ttattggcgc aaccactgaa aacccgtcgt ttgagcttaa ttcggcactg
    24601 ctttcccgtg cccgtgttta tctgttgaaa tccctgagta cagaggatat tgagcaagta
    24661 ctaactcagg cgatggaaga caaaacccgt ggctatggtg gtcaggatat tgttctgcca
    24721 gatgaaacac gacgtgccat tgcagaactg gtgaatggcg acgcgcgccg ggcgttaaat
    24781 acgctggaaa tgatggcgga tatggccgaa gtcgatgata gcggtaagcg ggtcctgaag
    24841 cctgaattac tgaccgaaat cgccggtgaa cgtagcgccc gctttgataa caaaggcgat
    24901 cgcttttacg atctgatttc cgcactgcat aagtcggtac gtggtagcgc acccgatgcg
    24961 gcgctgtact ggtatgcgcg aattattacc gctggtggcg atccgttata tgtcgcgcgt
    25021 cgctgtctgg cgattgcgtc tgaagacgtc ggtaatgccg atccacgggc gatgcaggtg
    25081 gcaattgcgg cctgggattg ctttactcgc gttggcccgg cggaaggtga acgcgccatt
    25141 gctcaggcga ttgtttacct ggcctgcgcg ccaaaaagca acgctgtcta cactgcgttt
    25201 aaagccgcgc tggccgatgc tcgcgaacgc ccggattatg acgtgccggt tcatttgcgt
    25261 aatgcgccga cgaaattaat gaaggaaatg ggctacgggc aggaatatcg ttacgctcat
    25321 gatgaagcaa acgcttatgc tgccggtgag gtttacttcc cgccggaaat agcacaaaca
    25381 cgctattatt tcccgacaaa caggggcctt gaaggcaaga ttggcgaaaa gctcgcctgg
    25441 ctggctgaac aggatcaaaa tagccccata aaacgctacc gttaatgtta tcgttgcggt
    25501 aatgttgtta ctgtatccct gtggtcgcag gctgtggcca catctcccat ttaattcgat
    25561 aagcacagga taagcatgct cgatcccaat ctgctgcgta atgagccaga cgcagtcgct
    25621 gaaaaactgg cacgccgggg ctttaagctg gatgtagata agctgggcgc tcttgaagag
    25681 cgtcgtaaag tattgcaggt caaaacggaa aacctgcaag cggagcgtaa ctcccgatcg
    25741 aaatccattg gccaggcgaa agcgcgcggg gaagatatcg agcctttacg tctggaagtg
    25801 aacaaactgg gcgaagagct ggatgcagca aaagccgagc tggatgcttt acaggctgaa
    25861 attcgcgata tcgcgctgac catccctaac ctgcctgcag atgaagtgcc ggtaggtaaa
    25921 gacgaaaatg acaacgttga agtcagccgc tggggtaccc cgcgtgagtt tgactttgaa
    25981 gttcgtgacc acgtgacgct gggtgaaatg cactctggcc tcgactttgc agctgcagtt
    26041 aagctgactg gttcccgctt tgtggtaatg aaagggcaga ttgctcgcat gcaccgcgca
    26101 ctgtcgcagt ttatgctgga tctgcatacc gaacagcatg gctacagtga gaactatgtt
    26161 ccgtacctgg ttaaccagga cacgctgtac ggtacgggtc agctgccgaa atttgctggc
    26221 gatctgttcc atactcgtcc gctggaagaa gaagcagaca ccagtaacta tgcgctgatc
    26281 ccaactgcag aagttccgct gactaacctg gtgcgcggtg aaatcatcga tgaagatgat
    26341 ctgccaatta agatgaccgc ccacacccca tgcttccgtt ctgaagccgg ttcatatggt
    26401 cgtgacaccc gtggtctgat ccgtatgcac cagttcgaca aagttgaaat ggtgcagatc
    26461 gtgcgcccgg aagactcaat ggcggcgctg gaagagatga ctggtcatgc agaaaaagtc
    26521 ctgcagttgc tgggcctgcc gtaccgtaaa atcatccttt gcactggcga catgggcttt
    26581 ggcgcttgca aaacttacga cctggaagta tggatcccgg cacagaacac ctaccgtgag
    26641 atctcttcct gctccaacgt ttgggatttc caggcacgtc gtatgcaggc acgttgccgc
    26701 agcaagtcgg acaagaaaac ccgtctggtt cataccctga acggttctgg tctggctgtt
    26761 ggtcgtacgc tggttgcagt aatggaaaac tatcagcaag ctgatggtcg tattgaagta
    26821 ccagaagttc tgcgtccgta tatgaacgga ctggaatata ttggctaata cccaattttt
    26881 ctgaatctaa aaagcgccta cgggcgcttt ttttgtctcc ctttgatacc gaacaataat
    26941 tactcctcac ttacacgtaa tactactttc gagtgaaaat ctacctatct ctttgatttt
    27001 caaattattc gatgtataca agcctatata gcgatctgct atagaaataa ttacacaata
    27061 cggtttgtta ctggaatcaa tcgtgagcaa gcttgagtga gccattatga aaacgaaaat
    27121 ccctgatgcg gtattggctg ctgaggtgag tcgccgtggt ttggtaaaaa cgacagcgat
    27181 cggcggcctg gcaatggcca gcagcgcatt aacattacct tttagtcgga ttgcgcacgc
    27241 tgtcgatagc gccattccaa caaaatcagg cgaaaaggtt atctggagcg cctgtacagt
    27301 taactgtggt agtcgctgcc cgctacgtat gcacgtcgtg gacggtgaaa tcaaatatgt
    27361 cgaaacggac aataccggcg atgacaatta cgacggcctg caccaggttc gcgcctgcct
    27421 gcgtgggcgt tccatgcgtc gccgtgtcta caatccggac cgcctgaaat atccgatgaa
    27481 acgagtcggg gcgcgcggtg aaggcaaatt cgagcgcatt agctgggaag aagcctacga
    27541 catcatcgcg accaatatgc agcgcctgat caaagagtac ggcaacgaat ccatctatct
    27601 gaactatggc accggtacgc tgggtggcac catgacccgc tcctggccgc cgggaaatac
    27661 cctggtcgcg cggctgatga actgctgcgg cggctatctg aaccattacg gcgactactc
    27721 ctccgcgcaa attgcggaag gtctgaacta tacctacggc ggctgggcag atggcaacag
    27781 cccgtcggat atcgaaaaca gtaagctggt agtgctgttt ggtaataacc ctggcgaaac
    27841 gcgaatgagt ggcggtgggg tgacttacta tcttgaacag gcacgccaga aatctaatgc
    27901 ccgcatgatc atcatcgatc cgcgctatac cgacaccggt gccgggcgcg aagatgagtg
    27961 gatccctatt cgtccgggaa cagatgccgc actggttaac ggtctggcgt acgtcatgat
    28021 cactgaaaac ctggtggatc aggcattcct cgataaatat tgcgttggct acgatgagaa
    28081 aaccctgcca gccagtgcgc cgaaaaatgg ccactataaa gcttatattc tgggtgaagg
    28141 gccagatggc gtggctaaaa caccggaatg ggcctcgcaa atcaccggtg ttccggcaga
    28201 caaaatcatc aaactggctc gtgaaatcgg cagtaccaaa ccggcgttta tcagccaggg
    28261 atggggcccg cagcgtcacg ctaacggtga aatcgcaacc cgtgctatct cgatgctggc
    28321 gattctgacc ggtaacgttg gtattaatgg agggaacagc ggcgcgcgtg aaggttcata
    28381 cagtttaccg tttgtccgta tgccgacctt ggaaaacccg atccagacca gcatttcgat
    28441 gtttatgtgg accgatgcca ttgaacgtgg cccggaaatg acggcgctac gtgatggtgt
    28501 gcgcgggaaa gataagctgg atgtgccgat caaaatgatc tggaactatg ccggtaactg
    28561 cctgattaac cagcattctg aaatcaaccg tacccatgaa atccttcagg atgataagaa
    28621 gtgcgagctg attgtggtta tcgactgcca catgacctca tcggcgaaat atgctgacat
    28681 cctgctgcct gactgcaccg cttccgaaca gatggacttt gcactggatg catcctgcgg
    28741 gaatatgtct tacgtgattt tcaacgatca ggtgattaaa ccgcgctttg aatgtaagac
    28801 catctatgaa atgaccagcg aactggcaaa acgtcttggc gttgagcaac agtttactga
    28861 aggccgtacc caggaagagt ggatgcggca tctgtatgcc cagtcgcggg aagcgattcc
    28921 tgaactgcca acgtttgaag agttccgcaa gcaggggatc tttaaaaagc gcgacccaca
    28981 agggcatcac gttgcttata aagccttccg tgaagatccg caggcaaatc cactgaccac
    29041 gccatcgggt aaaattgaga tttattcgca ggcgctggct gacattgccg ctacctggga
    29101 attgccagaa ggcgatgtga tcgatccact gccgatctac acgccgggct ttgaaagtta
    29161 tcaggatccg ctgaacaaac agtatccgct gcagcttaca ggtttccact ataaatctcg
    29221 cgttcactca acttacggca acgttgatgt gctgaaagcg gcttgccgtc aggaaatgtg
    29281 gatcaacccg cttgatgccc aaaaacgcgg tatccacaac ggcgataaag tcaggatctt
    29341 taacgatcgt ggtgaggttc atattgaggc gaaagtgacg ccacgaatga tgccgggtgt
    29401 ggtcgcactg ggtgaaggtg cctggtatga cccggatgca aaacgtgtcg ataagggtgg
    29461 ttgtattaac gtactgacca ctcaacgtcc gtctcctctc gctaagggga atccgtcaca
    29521 tacaaacctt gttcaggttg aaaaggtgta aggagtaacc gatgacaacc cagtatggat
    29581 tttttattga ttccagccgt tgcaccggtt gcaaaacctg cgagctggct tgtaaagact
    29641 acaaagattt gatgccagaa gtcagcttcc gccgcattta tgaatatgct ggtggcgact
    29701 ggcaggaaga taacggtgtc tggcaccaga acgtgtttgc ctactatctg tcgatttcgt
    29761 gcaaccactg cgaagatccg gcttgtacta aagtctgccc gagcggtgcg atgcataaac
    29821 gtgaagatgg ttttgttgtg gtcgatgaag atgtgtgcat tggctgccgc tactgccata
    29881 tggcttgccc gtatggcgca ccgcaatata acgaaacgaa aggccatatg accaaatgcg
    29941 atggttgtta tgaccgtgtt gcggagggta aaaagccgat ctgtgttgaa tcttgtccgc
    30001 tgcgggcgct ggatttcggc cctatcgacg agctgcgtaa aaaacatggc gatctggcgg
    30061 ccgttgcgcc gttgccgcga gctcacttta ccaaaccgaa tattgtgatc aaacccaatg
    30121 ccaatagccg cccgaccggg gataccactg gctatctggc aaacccgaag gaggtgtgag
    30181 atgggaagtg gatggcatga atggccgctg atgatcttca cggtcttcgg gcaatgtgtg
    30241 gcgggtggtt ttatcgttct ggctttggcg ctgcttaaag gcgacctgcg agcagaagcc
    30301 cagcagcgtg ttatcgcctg catgtttggc ttatgggtgc tgatgggcat tggctttatc
    30361 gcctctatgc tccatcttgg ttcaccgatg cgcgccttta actcgctcaa ccgggtaggg
    30421 gcttcagcac tcagtaacga aatcgccagc ggttcgatct tttttgctgt aggcggcatc
    30481 ggctggttgc tggcaatgct gaaaaagctg tcacctgcat tgcgtacgct atggctggtg
    30541 gtgacgatgg ttcttggcgt cgtctttgtc tggatgatgg tgcgtgtgta taacagcatt
    30601 gataccgttc cgacctggta cagcatctgg acgccgatgg gcttcttcct gacgatgttc
    30661 atgggcggcc cgttactggg ttacctgttg ctgagtctgg ctggcgtcga tggctgggcg
    30721 atgcgtctgc tgccagccat ttctgtactg gcactggtgg tgagtggcgt gatgtcagtg
    30781 atgcagggca cagagctggc aactattcat agttctgtgc agcaggccgc agcgctggtg
    30841 ccggactatg gtgcgctgat gtcctggcga atcgtgcttt tggccgttgc cctgtgcttg
    30901 tggattgcac cacagctaaa aggttatcag cctgcggttc cgctactatc cgtctcattc
    30961 attctgcttc tggcagggga attaatcggt cgcggcgtat tctatggttt gcatatgacc
    31021 gtggggatgg ccgtcgcaag ctaatcagta acaaccgggg tttcggcccc ggttctcttt
    31081 tatttctgct tcgttaacgt gtcataactg gtcatcaaat tacgataatc aggaatgtgg
    31141 ttagagaaca acgtcgccag tccttcaata tcattacgcc agtcacggtg cagctcgcaa
    31201 gccacgccga accatgtcat caattgcgca cctgcttgcg acatgcgatc ccatgccgaa
    31261 tgccgggtaa tttcattaaa ggtccctgac gcgtcagtca caacaaagac atcaaaccct
    31321 tcttcaatgg ctgaaagcgc cgggaatgcc acgcaaactt cggttaccac accggcaata
    31381 attaactgtt ttttacctgt cgctttgact gcttttacaa aatcttcgtt atcccaggcg
    31441 ttaatattgc cagggcgagc aatgtaaggt gcatcgggga attgtgcttt cagctctgga
    31501 actaatgggc cgttaggacc ggtttcaaaa ctggtggtaa gaatagttgg taagttgaaa
    31561 tacttagcca ggtcacccag cgccagcacg ttatttttaa acttatcggg ttcgatatcc
    31621 cgtacaaggg aaagtaaacc agcctggtga tcaacaagca aaacggcagc atcattttta
    31681 tcaagacgaa catacggttt ggtcatcctc ttctcctttc gagagtcagt ggtacgtctg
    31741 aaatcggtca gacgcgatgc attgctctga aagcatagac gggaaatatg agtttgctgt
    31801 gaccatgaaa tttttcgact gaacgaagcg tatgaaattt gtgttagatc aataaaaaca
    31861 atcagatgag attaatcatc tcttcagcat tagcgttatg aatgagtaat ataaatcgat
    31921 aataccgccg ctgcgatatg cgcgtatttt cacccattga caatgttttt tgcggtggca
    31981 tgatgcgcat gaaatttgaa cttcctcacg gttttaattc atgtccacgt atacccggcc
    32041 tgtcatgctt ttgctgtctg gcctgctttt gttgactctg gcgatcgcgg tgttaaatac
    32101 actcgtgccg ctttggctcg cccaggaaca catgtccaca tggcaggtag gcgttgtcag
    32161 ctcatcctat tttaccggca accttgtcgg tacattgctg acagggtatg tcattaaacg
    32221 cattggcttt aaccgcagct attatctggc ctccttcatt tttgccgctg gctgtgccgg
    32281 ccttggcctg atgattggtt tctggagctg gttggcgtgg cgttttgtcg cgggcgtcgg
    32341 ctgtgccatg atttgggtag tcgttgagag cgcgctgatg tgcagtggga cgtcacgtaa
    32401 ccgtgggcgt ttgcttgctg cgtatatgat ggtttattac gtgggaacat ttttaggcca
    32461 gttactggtc agcaaagtct caaccgagct gatgagcgta ttgccgtggg ttacaggttt
    32521 gacgttggca gggatcttac cgctgttgtt tacgcgtgtc ctgaatcagc aggctgaaaa
    32581 ccatgattcg acgtcaatta cgtcaatgct aaaactccgt caggcgcggc ttggcgtgaa
    32641 tggctgcatt atctcaggaa tcgtgctggg atctctatat ggcctgatgc cgctgtacct
    32701 caatcacaaa ggggtgagca atgccagcat tggtttctgg atggcggtac tggtcagtgc
    32761 gggtatcctc ggacaatggc cgattggacg tctggcggat aagtttggtc gactgctggt
    32821 gttgcgtgtt caggtctttg tcgtcattct cggcagtatc gcgatgctta gccaggcggc
    32881 gatggcccca gcgttattca tcctcggtgc cgctggcttt acgctatatc cggtggcgat
    32941 ggcctgggct tgtgagaaag ttgaacatca tcaactggtg gcgatgaacc aggccttact
    33001 gttgagctat accgtgggaa gtctgcttgg cccgtcattt accgctatgc taatgcagaa
    33061 tttctccgat aatttattgt ttatcatgat cgccagcgta tcgtttatct atttgctgat
    33121 gctgcttcgc aacgccggtc atacgccgaa acccgttgct cacgtgtaaa tgaattcaag
    33181 caaagtgtga acttactgtt tcacactctg cttttttgtt tcttctatct gacttgcttt
    33241 tttccaaatt ttattcgttt aaaaataaaa tgtgcagcag gttataattt tgcatttcgc
    33301 tatttccgca cttcttattt gccgcgcata atccctcgtt ttacagatgc ccctttaatt
    33361 ttggcgaagg atttgtctat ggctgggaat gttcaggaaa aacagctgcg atggtacaac
    33421 attgcgctga tgtcttttat cactgtctgg ggttttggca acgttgttaa taactatgcc
    33481 aaccaggggc tggtggttgt tttttcatgg gtgtttatct ttgcgctcta tttcacacct
    33541 tatgcactaa ttgttggtca gttaggctcg accttcaaag atgggaaggg cggggtcagt
    33601 acctggatta aacacacgat gggacccgga ttggcttacc tcgccgcgtg gacctactgg
    33661 gtggtgcata ttccctatct ggcacaaaaa ccccaggcaa tactgattgc gctcggttgg
    33721 gcgatgaaag gcgacggttc gctaatcaaa gaatattcag tcgtagcgtt acaggggtta
    33781 acgctggtgc tgtttatctt ctttatgtgg gttgcttcac gcggtatgaa atcgctgaaa
    33841 atcgtcggtt ctgtggcagg gattgcgatg tttgttatgt cgctcctgta tgtggcgatg
    33901 gcggtaaccg cgcctgcgat tactgaagtg catattgcga ccacaaacat tacctgggaa
    33961 acgttcattc ctcatatcga ctttacctac attaccacta tttcaatgct ggttttcgcg
    34021 gttggcggag ctgagaagat ttctccttac gttaatcaaa cgcgcaaccc aggaaaagaa
    34081 tttccaaaag ggatgttatg cctggcggtg atggttgcgg tttgtgccat tctgggctca
    34141 ctggcgatgg ggatgatgtt tgactcgcgt aatatcccgg atgacttaat gactaacggt
    34201 cagtattacg cctttcagaa gctgggcgag tattacaaca tgggtaatac tttaatggtg
    34261 atttacgcca ttgcgaatac cctgggacaa gtagctgcgc tggtattctc gattgatgcc
    34321 ccgcttaaag tgctattagg cgatgctgac agcaaatata ttccagccag tttatgtcgt
    34381 accaacgctt ctggtacgcc cgttaatggc tattttctga ccctggtgct ggtggcgatc
    34441 ctgattatgc tgccgactct cggcatcggt gatatgaaca atctctataa atggctgttg
    34501 aatcttaact cggtagtgat gccgctgcgt tatttatggg tatttgttgc atttattgca
    34561 gtcgttcgcc tggcgcagaa atatacacca gagtatgtct ttattcgtaa taagcctctg
    34621 gcaatgaccg tcgggatttg gtgttttgcc tttactgcct ttgcctgttt gacggggatc
    34681 ttcccgaaaa tggaagcctt cactgcagag tggaccttcc agttggcgct gaatgttgca
    34741 acgccgtttg tgctggtagg attaggactg atattcccgc tgctggcgcg taaagcgaat
    34801 agtaaataat tattgtggtc ggtgacgctg actctggcag atctgacccg tttttacata
    34861 atcgagaaat gcgcgcaagc cgcaggacat atactggcgg tttgggtaat acatttggaa
    34921 gctgtgctgt aagggcgtta gaaatagagc tctgaattat ttatgcagcg cggaataaaa
    34981 aagcggcaac tcaataaagt tgccgcttta cggggaaatt agaacattac cttatgaccg
    35041 tactgctcaa gaataccttt cacgcgttcc atggtttctt tcttcggtgg tttaacacca
    35101 tcgagtttgt attcttcacc catcgccacc catttgtgtt tgcccagttc gtggtagggg
    35161 agaagttcga ttttctcaac gttacccata tcacgggtaa attcaccgag gcgatgcgct
    35221 gaatcgtcat cgtcagacca gcctgggaca acaacgtagc ggatccacac cttcacattt
    35281 ttattcgcca gatatttggc gaactccagc gtgcggtggt tggaaactcc aaccagattt
    35341 tggtggatct cgtcgttcat ctgtttgaga tcgagcatta ccaggtcggt tacttccagc
    35401 agttcatcaa tcaccggatc gtaacgacga acaaaaccgt tggtgtccag acaggtatga
    35461 atgccttctt ttttgcaggc gcggaaccag tcacgaacaa actcagcttg caggattgct
    35521 tcaccgccgg atgcggtaac gccgccgccg gaagcgttca taaagtggcg ataggtcacc
    35581 acttccttca tcaaatcttc aacggtaact tctttaccgc catgcgtgtc ccaggtgtcg
    35641 cggttatgac aatacaggca gcgcatcagg cagccctgga aaaaggtgat aaagcgaata
    35701 cctgggccgt ctacggttcc acaggattca aaggagtgaa tgcgaccaat aactgacatt
    35761 gcggtgtttc tccagatgtg gcccatctga ggccgtgttg gtgcgcagct cgaaggctac
    35821 gtcgagtctg ttttggcagt caccttaaag tatagatagc tgacaaaaaa ggctcacgtg
    35881 ctaaaaaagg ccccactttc gtggagcctt tattgtacgc tttttactgt acgatttcag
    35941 tcaaatctaa ttacatagat tgagtgaagg tacgagtaat aacgtcctgc tgctgttctt
    36001 tagtcagcga gttgaaacgt actgcgtagc cagatacacg gatggtcagc tgcggatatt
    36061 tttccggatt ttccatcgcg tcgagcagca tttcacggtt catcacgtta acgttcaggt
    36121 gctgaccacc ttcgatggat gcttcgtggt ggaagtaacc atccatcaga ccagccaggt
    36181 tggtcttacg aacttcgtcg tctttaccca gtgcgttcgg aacgatagag aaggtgtagg
    36241 agataccatc tttagcgtaa gcaaacggca gtttagcaac ggaagtcaga gacgctacag
    36301 cacctttctg gtcacgaccg tgcatcgggt tagcacccgg tccgaacggc gcgccagcac
    36361 gacgaccgtc tggggtgtta cccgttttct taccatacac aacgttagaa gtgatggtca
    36421 gaacagactg agtcgggata gcgtcacggt aggtgtgcag tttctgaatt ttcttcatga
    36481 aacgttctac caggtcaaca gccaggtcat ctacacgcgg atcgttgtta ccaaactgcg
    36541 ggtattcgcc ttcgatttcg aagtcgatag ccagaccgtc ttcgtcacga atcggtttaa
    36601 ctttcgcata tttgattgca gacagggagt cagcagcaac ggacagacca gcgataccac
    36661 acgccatggt gcggataacg tcacggtcgt gcagcgccat cagagaggct tcgtagctgt
    36721 acttgtcgtg catgtagtga atgatgttca gtgcagtgat gtactgttta gccagccagt
    36781 ccatgaagtg atccatgcgc tccatcactt catcgtagtt caggagatcg cctttgatcg
    36841 gttcagactt cggaccaacc tgcattttca gtttttcgtc aacgccgccg ttgattgcgt
    36901 acagcatggt tttcgccagg tttgcacgcg caccgaagaa ctgcatttgt ttaccaacga
    36961 tcatcgggct tacgcagcaa gcgatagcgt agtcatcgtt gttgaagtcc ggacgcatca
    37021 ggtcatcgtt ctcatactgc agagaagagg tgtcgatgga cactttagcg gcgaatttct
    37081 tgaagttcag cggcagtttt tcagaccaca gaatggtcat gttcggttcc ggagacggac
    37141 ccatggtgta cagggtgttc aggaaacgga agctgttttt ggtaaccagg gtacgaccgt
    37201 cgaggcccat accaccgata gattcggttg cccagatcgg gtcgccagag aacagttcat
    37261 cgtactccgg agtacgcagg aagcgaacca tacgcagttt catgaccagg tggtcaacca
    37321 tttcctgcgc ttcttgttcg gtgatcttgc cagctttcag gtcacgttcg atgtacacat
    37381 ccaggaaggt ggaggtacga ccgaaggaca ttgcagcacc gttctgagac ttaacagcag
    37441 ccaggtagcc gaagtaagtc cactggatag cttcctgagc gttggtagcc ggaccagaga
    37501 tgtcgtagcc gtatttcgca gccatttctt tcatctgacc cagagcgcgg tgctgttcag
    37561 cgatttcttc gcgcagacgg atagtctgtt ccaggtttac gccgttttcc agatcagcct
    37621 gcagagaggt gaactgagcg tatttgtctt tcatcaggta gtcgataccg tacagcgcaa
    37681 cgcgacggta gtcaccgatg atacggccac ggccataagc atctggcaga ccggtcagaa
    37741 caccggattt acggcaacgc aggatgtccg gagtgtaaac gtcgaacacg ccctggttgt
    37801 gagttttacg gtattcagtg aagatttttt tgatcatcgg gtccagttcg cggttgtacg
    37861 ctttgcagga accttcgatc attttgatac caccgaacgg gataagagca cgtttcagcg
    37921 gagcttcagt ctgcagacca acaacttttt ccaacgcttt gttgatgtag ccagcgtcgt
    37981 gagaggtgat ggtggaagca acagcggtgt cgaagtcaac tggcgcgtga gtgcggtttt
    38041 ccagtttaac gccttccatt actttgtccc acagggtggt ggtcgcttca gtagcgccag
    38101 ccaggaagga ctcgtcaccc tcgtacggag tgtagttttt ctgaatgaag tcacggacgt
    38161 ttacttcatt ctgccagtca cctttggtaa aaccttccca ggctgtggct aacttttcat
    38221 taagctcgga catgtaacac ctaccttctt aagtggattt tttatttact gcgtacttcg
    38281 acaaccatta atggtgatcg ttttcacgca ggtaaatgac ccagtatgtc aacccaacca
    38341 acaaaccacc gccgataata ttaccgatcg taaccggaat caggttatca gtgatgaagt
    38401 tcatcacggt caggtgagaa aaattttccg gtgcagaacc gacagcggtc cagaattccg
    38461 gagatgcgaa gtcgcggatt acaataccca tcgggatcat aaacatgttt gcgatactgt
    38521 gctcaaaacc gctggcaaca aacatcgcga ccggcagcac catgataaac gctttgtcca
    38581 tcaggctgcg gccagaataa ctcatccata ctgccagaca taccatcagg tttgccagga
    38641 taccaaggca gacggcctca ataaaagtat ggtgcacttt gtggtcggcg gtttgtagga
    38701 cgtttagtcc ccattgacca tttgcggtca tatactcgcc ggaaagccac attaaaagta
    38761 caaacagcag tgcgccgacc aggttgccaa aatagacatt tagccagttt ttagccaact
    38821 gaccccaggt gatgcgccca ctcgccttag caacaacaat caacactgtg gaagtaaaga
    38881 gatcggctcc gcagacaaca caaagaatca gtcccagaga gaagcaaatg ccgccaacca
    38941 gttttgccat gccgaagggc attgtgcctg tgccagtggt tgctgtgata tagaagacga
    39001 atgcgattga gatgaaaaca ccggcggtaa tcgccagata gaaagtctta agcggatgtt
    39061 tcgttgcttt atagacaccc gcctcttcgg ccactttggc cattgcagca ggaagtaaaa
    39121 gatcaaaagg gttgtcagct ttcacactaa ctctctcttt attaagtcgg cgacgagata
    39181 ctaacaaagc attatagatg agaaattgat atagatcata tctcgcctgg cttataggcc
    39241 cgtaactc
//
LOCUS       DBFIFI010000021        38351 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.021, whole genome shotgun sequence.
ACCESSION   DBFIFI010000021 DBFIFI010000000
VERSION     DBFIFI010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38351
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.021"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..595)
                     /gene="yncG"
                     /locus_tag="N7B39_001087"
     CDS             complement(<1..595)
                     /gene="yncG"
                     /locus_tag="N7B39_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HGU2421106.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQVPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWASDAPEQLKKNVIEYRKSLYVWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNATNISAIADAVCQLPTLQEI"
     gene            862..2361
                     /gene="ansP"
                     /locus_tag="N7B39_001088"
     CDS             862..2361
                     /gene="ansP"
                     /locus_tag="N7B39_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HGU2421107.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            complement(2476..3537)
                     /gene="yncE"
                     /locus_tag="N7B39_001089"
     CDS             complement(2476..3537)
                     /gene="yncE"
                     /locus_tag="N7B39_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HGU2421108.1"
                     /translation="MHLRHLFSLRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKDSVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            3779..5881
                     /gene="pqqU"
                     /locus_tag="N7B39_001090"
     CDS             3779..5881
                     /gene="pqqU"
                     /locus_tag="N7B39_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HGU2421109.1"
                     /translation="MKIVFVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDDASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSSRDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGSVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGMEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PAPGRNYGVGVNIAWRFE"
     gene            complement(5917..6582)
                     /gene="mcbR"
                     /locus_tag="N7B39_001091"
     CDS             complement(5917..6582)
                     /gene="mcbR"
                     /locus_tag="N7B39_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HGU2421110.1"
                     /translation="MPGTEKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(6780..7817)
                     /gene="curA"
                     /locus_tag="N7B39_001092"
     CDS             complement(6780..7817)
                     /gene="curA"
                     /locus_tag="N7B39_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HGU2421111.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQSGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQREMGQWVKEDKIHYREEITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            7998..8516
                     /gene="mddA"
                     /locus_tag="N7B39_001093"
     CDS             7998..8516
                     /gene="mddA"
                     /locus_tag="N7B39_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HGU2421112.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            8513..8962
                     /gene="ydcZ"
                     /locus_tag="N7B39_001094"
     CDS             8513..8962
                     /gene="ydcZ"
                     /locus_tag="N7B39_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGU2421113.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNSLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(8963..9196)
                     /gene="ydcY"
                     /locus_tag="N7B39_001095"
     CDS             complement(8963..9196)
                     /gene="ydcY"
                     /locus_tag="N7B39_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HGU2421114.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(9282..9455)
                     /gene="ortT"
                     /locus_tag="N7B39_001096"
     CDS             complement(9282..9455)
                     /gene="ortT"
                     /locus_tag="N7B39_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HGU2421115.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            9650..9745
                     /gene="yncL"
                     /locus_tag="N7B39_001097"
     CDS             9650..9745
                     /gene="yncL"
                     /locus_tag="N7B39_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HGU2421116.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(9842..11266)
                     /gene="patD"
                     /locus_tag="N7B39_001098"
     CDS             complement(9842..11266)
                     /gene="patD"
                     /locus_tag="N7B39_001098"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HGU2421117.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTAPSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTVACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTLFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(11288..12082)
                     /gene="ydcV"
                     /locus_tag="N7B39_001099"
     CDS             complement(11288..12082)
                     /gene="ydcV"
                     /locus_tag="N7B39_001099"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU2421118.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLMALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLESGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(12072..13013)
                     /gene="ydcU"
                     /locus_tag="N7B39_001100"
     CDS             complement(12072..13013)
                     /gene="ydcU"
                     /locus_tag="N7B39_001100"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU2421119.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            complement(13014..14027)
                     /gene="ydcT"
                     /locus_tag="N7B39_001101"
     CDS             complement(13014..14027)
                     /gene="ydcT"
                     /locus_tag="N7B39_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU2421120.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFICVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            complement(14045..15190)
                     /gene="ydcS"
                     /locus_tag="N7B39_001102"
     CDS             complement(14045..15190)
                     /gene="ydcS"
                     /locus_tag="N7B39_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HGU2421121.1"
                     /translation="MSKTFARSSLCALSMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLQDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRDQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQTNALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASSL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            complement(15435..16841)
                     /gene="ydcR"
                     /locus_tag="N7B39_001103"
     CDS             complement(15435..16841)
                     /gene="ydcR"
                     /locus_tag="N7B39_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HGU2421122.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKVPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQEYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHNDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWLWG
                     EREEQAVKQLGKLIQERL"
     gene            complement(16920..17336)
                     /gene="hicB"
                     /locus_tag="N7B39_001104"
     CDS             complement(16920..17336)
                     /gene="hicB"
                     /locus_tag="N7B39_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HGU2421123.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            complement(17382..17558)
                     /gene="hicA"
                     /locus_tag="N7B39_001105"
     CDS             complement(17382..17558)
                     /gene="hicA"
                     /locus_tag="N7B39_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin HicA"
                     /protein_id="HGU2421124.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIK
                     EPLRKAILKQLGLS"
     gene            17780..18010
                     /gene="yncJ"
                     /locus_tag="N7B39_001106"
     CDS             17780..18010
                     /gene="yncJ"
                     /locus_tag="N7B39_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HGU2421125.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            complement(18102..20063)
                     /gene="rlhA"
                     /locus_tag="N7B39_001107"
     CDS             complement(18102..20063)
                     /gene="rlhA"
                     /locus_tag="N7B39_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HGU2421126.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVVKVAKDHLDV
                     AVTEPLANGDGLNVLIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(20136..20672)
                     /gene="sutR"
                     /locus_tag="N7B39_001108"
     CDS             complement(20136..20672)
                     /gene="sutR"
                     /locus_tag="N7B39_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310064.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HGU2421127.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVIISLFPYDPQLCFEHF
                     SIQMAPGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            20764..21937
                     /gene="ydcO"
                     /locus_tag="N7B39_001109"
                     /pseudo
     CDS             20764..21937
                     /gene="ydcO"
                     /locus_tag="N7B39_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310065.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
     gene            complement(21972..22640)
                     /gene="ydcL"
                     /locus_tag="N7B39_001110"
     CDS             complement(21972..22640)
                     /gene="ydcL"
                     /locus_tag="N7B39_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HGU2421128.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(22944..23537)
                     /gene="tehB"
                     /locus_tag="N7B39_001111"
     CDS             complement(22944..23537)
                     /gene="tehB"
                     /locus_tag="N7B39_001111"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HGU2421129.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGEYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWEMVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            complement(23534..24526)
                     /gene="tehA"
                     /locus_tag="N7B39_001112"
     CDS             complement(23534..24526)
                     /gene="tehA"
                     /locus_tag="N7B39_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HGU2421130.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTLALLMQGKLLVRTERAVLMKAEDKE"
     gene            24650..25630
                     /gene="ydcK"
                     /locus_tag="N7B39_001113"
     CDS             24650..25630
                     /gene="ydcK"
                     /locus_tag="N7B39_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HGU2421131.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKNVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     INQGAYISDSVTIHDSLVCGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVIRYAFIEHRAEVFDFASIEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            complement(25625..26161)
                     /gene="rimL"
                     /locus_tag="N7B39_001114"
                     /pseudo
     CDS             complement(25625..26161)
                     /gene="rimL"
                     /locus_tag="N7B39_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
     gene            complement(26224..26448)
                     /gene="ydcH"
                     /locus_tag="N7B39_001115"
     CDS             complement(26224..26448)
                     /gene="ydcH"
                     /locus_tag="N7B39_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HGU2421132.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDDRGY
                     NAEVVRMKKQKLQLKDEMLKILQHESVKEV"
     gene            complement(26588..28243)
                     /gene="opgD"
                     /locus_tag="N7B39_001116"
                     /gene_synonym="mdoD"
     CDS             complement(26588..28243)
                     /gene="opgD"
                     /locus_tag="N7B39_001116"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HGU2421133.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            complement(28468..29811)
                     /gene="ydcJ"
                     /locus_tag="N7B39_001117"
     CDS             complement(28468..29811)
                     /gene="ydcJ"
                     /locus_tag="N7B39_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGU2421134.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYDQRGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVAAQPITYEDFLPVS
                     AAGIFQSNLGNETQARSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            30028..30951
                     /gene="ydcI"
                     /locus_tag="N7B39_001118"
     CDS             30028..30951
                     /gene="ydcI"
                     /locus_tag="N7B39_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGU2421135.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGPNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            complement(30989..32629)
                     /gene="trg"
                     /locus_tag="N7B39_001119"
     CDS             complement(30989..32629)
                     /gene="trg"
                     /locus_tag="N7B39_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HGU2421136.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRTLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMKDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHYA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLNKQSVLAEPRGAGELVSFAPV"
     gene            33028..33177
                     /gene="hokB"
                     /locus_tag="N7B39_001120"
     CDS             33028..33177
                     /gene="hokB"
                     /locus_tag="N7B39_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HGU2421137.1"
                     /translation="MKRNPLVVCLLIICITILTFTLLTRQTLYELRFRDSDKEVAALM
                     ACTSR"
     gene            complement(33155..33223)
                     /gene="yncP"
                     /locus_tag="N7B39_001121"
     CDS             complement(33155..33223)
                     /gene="yncP"
                     /locus_tag="N7B39_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HGU2421138.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(33249..33422)
                     /locus_tag="N7B39_001122"
     CDS             complement(33249..33422)
                     /locus_tag="N7B39_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2421139.1"
                     /translation="MKKLALILFMGALVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(33667..34197)
                     /gene="cybB"
                     /locus_tag="N7B39_001123"
     CDS             complement(33667..34197)
                     /gene="cybB"
                     /locus_tag="N7B39_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HGU2421140.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            34386..35387
                     /gene="gap"
                     /locus_tag="N7B39_001124"
     CDS             34386..35387
                     /gene="gap"
                     /locus_tag="N7B39_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HGU2421141.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYKVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(35429..36868)
                     /gene="aldA"
                     /locus_tag="N7B39_001125"
     CDS             complement(35429..36868)
                     /gene="aldA"
                     /locus_tag="N7B39_001125"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HGU2421142.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(37065..37865)
                     /gene="ydcF"
                     /locus_tag="N7B39_001126"
     CDS             complement(37065..37865)
                     /gene="ydcF"
                     /locus_tag="N7B39_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HGU2421143.1"
                     /translation="MNITPFPTLSTATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDVPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            complement(37981..>38351)
                     /locus_tag="N7B39_001127"
     CDS             complement(37981..>38351)
                     /locus_tag="N7B39_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2421144.1"
                     /translation="RFINITPTSLSFKHADLSGLSYKNARLELALICPAIKSEVKISF
                     DWIHSFRVTDEGDLLGMSGKQGINQTGIYRVANSTYLTWFTMQSCEIHKNKMIEHYVI
                     ATSNDVIDVLSTVSPQIVYD"
BASE COUNT         9571 a         9779 c         9213 g         9788 t
ORIGIN      
        1 gtatttcttg taacgttggt aactgacata ctgcatcagc aatcgcactg atattcgtgg
       61 cattatcctg aaaccattca tgcccaggcc cccatgtgcg catagtgcaa agataacagt
      121 ccaccagcgt tagttgctca ccaaacgcat aaggttcagc ggtcaattgt gaattcagcc
      181 agacataaag cgatttacga tattcaataa cattcttctt taactgttca ggtgcgtcag
      241 atgcccaacg ctcagggtaa tcggcgaaag tgaacgttgg atagacatta gcaaccagcc
      301 agaccaataa ccgttgaaac tgctgacgtt cggcacgccc aaccggtggc gcaaggtccg
      361 ggcgacgatc gagaaccatc aatgcaatcg ccgctgtctc cgtcataatc tcgtcatttt
      421 ccagtgccag tgtcggaacc tgacacaacg ggttcagggt tttcaataac tcgcgtgaag
      481 ttccctcgtg gtcaaaacca ctgacatcaa cgaactgata aggaatatca gccagtgtta
      541 gcatcagctc actgattgta gagccccatc cgggtacgcc atacacctta atcatatgcc
      601 cctcacaatc ttttaaagat taaatgtagg acagtacaaa ggttaaccag caaaaggatt
      661 tgtcattttt tcgtcagttg tattaatccc cttcaggatt gtaaggggat gttttgcatc
      721 gtttatcgat aacgggtagg atgctgcgcc gatagtttta tacccataaa gaataatggt
      781 gataactatc atcgccagga tgaataaaca ttgttcatgg caacttatat gactttttca
      841 ttaaagcaat cagggagagc aatgagtaaa cacgacaccg acacttcaga tcaacacgcc
      901 gcgaaacgcc gctggcttaa tgcccacgaa gaggggtatc acaaagcgat gggcaatcgc
      961 caggtacaga tgatcgccat tggcggcgcg attggcaccg gcttgttttt aggtgcagga
     1021 gcccgactgc aaatggcggg tccagcactg gcactggttt atttaatttg tggcttgttt
     1081 tcgtttttta ttctgcgtgc attgggtgag ctggtgctac accgcccttc cagtggcagt
     1141 tttgtctctt atgcccgtga gtttttgggt gagaaagccg cttatgttgc tggctggatg
     1201 tacttcatca actgggcgat gacggggatt gttgatatta ccgccgtcgc tctgtatatg
     1261 cattactggg gtgcgtttgg cggcgtgccg cagtgggtct ttgcgctcgc tgcacttacc
     1321 atcgttggca ccatgaatat gatcggtgtg aaatggtttg cggagatgga gttctggttt
     1381 gcgcttatta aagtgctcgc cattgtgacc tttttggtcg tgggtacggt gttcctcggt
     1441 agtggtcagc cgctggatgg caacaccact ggctttcatt taatcaccga taatggcggc
     1501 ttcttccccc acggtttgct gcctgcgctg gtgttgattc agggcgtagt gtttgctttt
     1561 gcctccattg aaatggtggg tacagctgcc ggagaatgta aagatccgca gaccatggtg
     1621 cctaaagcga ttaacagtgt gatttggcgt attggcctgt tttacgtcgg ctccgtggtg
     1681 ttgctggtta tgttattgcc gtggagcgcg tatcaggcgg ggcaaagtcc gttcgtgacg
     1741 tttttctcta aactgggtgt gccatatatc ggcagcatta tgaacattgt ggtgctgacc
     1801 gctgccctct ccagcctgaa ttcaggtctg tactgcaccg gacgtattct gcgctcaatg
     1861 gcgatgggcg gttccgcacc gagttttatg gcgaaaatga gtcgtcagca tgtgccgtat
     1921 gccgggattc tggcgacact ggttgtgtat gtcgtcggcg tattcctcaa ctatctggtg
     1981 ccgtcgcgcg tatttgagat tgtgttgaac ttcgcgtcgc tgggaatcat cgcttcatgg
     2041 gcgtttatca tcgtgtgcca gatgcgcctg cgtaaagcga ttaaagaagg caaagcagcg
     2101 gatgtcagtt ttaaactgcc tggcgcgccc ttcacttcct ggctgacatt actgttttta
     2161 ctgagtgtcc ttgtgctgat ggcgttcgat tacccgaacg ggacttatac tatcgcggcg
     2221 ctgccgatta tcggtattct gctggttata ggctggtttg gtgtgcgcaa acgcgttgct
     2281 gaaattcaca gcactgcgcc agtcgtcgag gaagatgaag aaaaacagga aattgtgttt
     2341 aagcctgaaa cggcgagcta attgcaaaat tgcccggcta cgtgccgggc aaatttagat
     2401 tactgacgaa ggtgtgccgc atgcggcgtg atcaaagtca ggcaagtctc tccgttatca
     2461 ccctcgccat atctattaca gcgcaatacg aatcacatcg tctggctggg tagcttcctg
     2521 ctgtttagtg gatttttgtt tcacactgac atacagcgtt ttgccatcgg cagacagcgc
     2581 caggctgttc ggatgagtcg gcgtatcgaa cgttttcacc actttatagc ttttcgcgtc
     2641 aatcacactg actttacctg cctgacgatg cgttacgtag gcttcattac gcgctgggtt
     2701 aaacagcaca gccagtgatt ccggtgccgc aaccttcgcc agaatattgc cattacgggt
     2761 atcgaccact aacacttctg ccgctttaga atcggtgata aatgcacgct gcctggcggt
     2821 atcaaggctg atgttgataa agaagtgctc tttgccgtca tccagcagct ttttacggct
     2881 gaggatttta ttgtcggcgg tgtcgatggt aatcaattcg ccgtcagcgt tagtggtgta
     2941 aagacgtttg cctttgctat ccagtgccag accggtactc attttaccgg tgttctggat
     3001 ggcggttttc agtttgatat tctcgccatc aacgacccaa atcacgctat ctttaccaat
     3061 accactgatg taaacggtgt tcgtggcatc atcagctacc agctcacgcg gttgcagcgg
     3121 gcgcacctct tccgtgcgct tacgatcatc cagcaccaga cggcctttca cctcgccagt
     3181 tttggcatct atcgccgtga ccgcgctgtt tacggtgtta ccaaaccaca acgtctgagt
     3241 cgtgttattg atggtggcac caaacggctt gagatcgtta tggatcgcct gcgtcacttc
     3301 cagagtgacc ggatcaagac gataaaccac gccgccttta tccagtttgc ggctttgcga
     3361 agtggcgagc cacagcgcgt tttcttgctg gctataagcc atttcgtagg cacctttacc
     3421 taccgcttta cgcagcattt cttctgcggc ctgcgtactg aatgatgaag caacaagcaa
     3481 tgaacctaac agtaatgaac cacgcaggcg caatgaaaac agatgacgta aatgcatgac
     3541 gactcccttt gatgaaccga tatttttgct gcttcacaat gcttcgctaa gcgtaatcat
     3601 cgttacgctc ttgttataaa tgggaatggt aatcattatt tactcaaatg tggagcattt
     3661 cttgccgtta cgactacaat tctctcaatt aattaacgta tcgtgccgtt tcgttttcca
     3721 taaatttaca atttcattac catgacggtg ttttcccatt cttaacctgg cttttttcat
     3781 gaagattgtt ttcgttcgac agaccgtttt gcccgccctg cttgtccttt cccccgttgt
     3841 ttttgccgct gatgaacaga ccatgattgt cagtgccgcg ccgcaggtgg tttcagaact
     3901 ggatacccct gcagcagtaa gcgtggtgga tggcgaggag atgcgcctgg caacaccgcg
     3961 cattaacttg tccgaatcac tgaccggcgt gcctggtttg caggtacaaa accggcagaa
     4021 ctatgcgcaa gatttacagc tgtcgattcg tggatttggc tcccgctcca cttacggaat
     4081 tcgcggtatt cgcctgtatg tggacggtat tcccgctacc atgcccgacg ggcaagggca
     4141 aacatccaac atcgatttaa gcagtgtgca aaatgtggaa gtgctgcgtg gccccttctc
     4201 tgccctgtat ggcaacgcgt ctggcggtgt aatgaatgtc accacccaga ccgggcaaca
     4261 gccaccaacc attgaagcca gtagttacta cggcagtttt ggcagctggc gctatgggct
     4321 gaaagcaacg ggcgcaacgg gagacggcac acagcctggc gatgttgatt acaccgtctc
     4381 aaccacgcgt tttacgaccc acggctatcg tgaccatagt ggcgcacaga aaaatttagc
     4441 taatgccaaa ctgggcgtac gcattgatga cgccagcaaa ttaagcctga ttttcaatag
     4501 cgtggatatc aaagcagatg acccaggtgg gctaaccaaa gcagaatgga aagcgaatcc
     4561 gcaacaagcg cctcgtgcag aacagtacga cacgcgaaaa accatcaagc aaactcaggc
     4621 tgggttgcgc tatgaacgta gcctgagttc gcgggatgat atgagtgtga tgatgtatgc
     4681 cggagagcga gaaacgaccc agtaccagtc aatacccatg gcaccacaac ttaacccgtc
     4741 acatgcgggc agcgtgatta ccctgcaacg ccattaccag ggaatagaca gccgctggac
     4801 acaccgtggt gaactgggcg ttccggtcac gttcactacc ggcctgaact acgaaaacat
     4861 gagtgaaaac cgcaagggct acaataactt ccgcctgaat agcggcatgc cggagtacgg
     4921 gcaaaaaggt gagttgcgtc gcgacgaacg caatctgatg tggaacatcg atccctattt
     4981 acagacgcag tggcagctga gcgaaaaact gtcgctggat gctggcgtgc gctacagctc
     5041 cgtgtggttt gattccaacg accattacgt tactccgggt aacggcgatg acagcggtga
     5101 tgccagttat cacaaatggc taccagccgg atcgttaaaa tatgcaatga ccgatgcctg
     5161 gaatatctat ctggcagccg ggcgtggttt tgaaacgccg acgattaatg agctgtctta
     5221 tcgcgctgat gggcaaagcg gtatgaactt tggtttaaaa ccatccacca acgatacaat
     5281 tgagatcggc agtaaaacgc gtattggtga tgggctgctt agtctcgcat tgttccagac
     5341 cgacaccgat gatgaaattg ttgtcgatag cagtagcggt gggcgtacga cttacaaaaa
     5401 tgctggaaag acccgtcgtc aaggcgctga actggcatgg gatcaacgtt tcgcgggaga
     5461 ttttcgcgta aacgcgtcct ggacctggct tgatgcgacc tatcgcagca atgtttgcaa
     5521 cgaacaggat tgtaacggta atcggatgcc agggatcgcc cgtaatatgg gctttgcgtc
     5581 gataggttat gtaccggaag atggttggta tgcaggcatg gaagcgcgtt atatgggcga
     5641 tattatggca gatgatgaaa atacggccaa agcgccgtct tatactctcg tcggcttatt
     5701 caccgggtat aaatacaatt accacaattt aactgtggat ttgtttggtc gtgtcgataa
     5761 tttattcgat aaagaatacg ttggttctgt cattgtcaat gagtcaaacg ggcgatatta
     5821 cgaacctgcg cccgggcgaa attatggtgt cggcgtgaat atcgcatggc gatttgagta
     5881 attaccttga tgcccggtat ttgccgggca tttactttaa cgattgtatt gctggtataa
     5941 aatagcaata ttttgttgca taatttcagc aagacaatgt ctgcatccct ctttgtcttt
     6001 tgcttttaac gcagcgagta acagatgata gttttctata tgctcccgta attccgctgg
     6061 attaatcgct tcatagagat aatgtaaacc aggtcccatc ctgacccaca gttgctcaat
     6121 catctcacac aggatgggca tatttgagcg atgataaatc gctaagcgaa atagcctgtt
     6181 tacattaatg atttgttcca tgtcgccctt ttcttgcgcc tgctgtaatt tctcgagcaa
     6241 ttcctggagt tccgccaggt cttgcggggt gaggttttca acagccagag caactgccat
     6301 taattccagt tcgtagcgga tccgattgat ttcgtctaat tgacgtttcc ccacttccgg
     6361 aactgtaaat gcttgcgctg gtgcgacaga aagcgcattc accgaaacca gacgtaataa
     6421 tgcttcacgc acaggtgtaa tactcatacc taattgctcc gccagatttt tagtaatcag
     6481 gcgtgcgcca ggttttagtg cgccaatact aagctgatgt ttcaggtcgt tctcaacctg
     6541 cagagtcaaa ctgacatgtt tcattttttc cgttccaggc attttaattc cttcgcgtgt
     6601 tttctgtcca accctgcctg catggcagaa ggaattcacc tggtgtatta aagtgaataa
     6661 tcataaacca ctcacctttg ttgttattag cagcagaaca atgatgtggt tagttaatta
     6721 aagctgaaaa taagcttttt gcctggattt ttatggcggc ttacgccgcc gttgaaactt
     6781 taatcatcac ccgccacgcg gatcaccact ttgccgaagt ttttaccctt cagcaggccg
     6841 ataaacgtct gtggcgcatt ctctaaaccg tcagtaattt cttcgcggta gtggatttta
     6901 tcctctttca cccattgccc catctccctc tgaaactcat ggatgcggtg accataatcc
     6961 tgagcgataa taaaaccttg caagcgaata cgttttttca gcactgtagc catcaacaga
     7021 ggtaaacgat ccggaccggg tggtagctct gtagcgttat agctgctcac taatccgcag
     7081 acgggaatgc gcgcagatgt attaagtaac ggtagcaccg catcgaatac cttaccgccc
     7141 acgttttcat aatagatatc aatacctttt gggcacgctt tcgccagttg ttcggcaaaa
     7201 tcatccgcgt ggtgatcaag acaaacatcg aagcctaaca cctcggtagc atggcggcat
     7261 ttttccgcgc caccggctac ccccaccact ctgcaacctt taagtttgcc gatttgcccc
     7321 accgtcgccc ccactggtcc tgtcgccgca gctaccacca acgtttcgcc ctctttaggc
     7381 tgaccgatat ccagtaggcc catataagcg gtaaagcctg gcatccctag cacacccagc
     7441 gaccacgatg gattttgcgg atgatcgcca agtttcacca gatcatcacc actggatatg
     7501 tcatagtctt gccatccact gtagcccagc acccagtcgc cagactgata atcaggatga
     7561 ttcgactcca cgacacggct caccgtaccg ccgaccatca cgccgccaat atcaaccggc
     7621 ggtgaataag atggctcatc gctcatacgt ccacgcatat acgggtccag ggacaaataa
     7681 actgtgcgca gtaacacctg tccttcaccc ggtgtggcga catcatcttc ttcaagacgg
     7741 aaattctccg gaaccggtgc gccatgtgga cgcgaggcca gaacccaacg tcgattacgc
     7801 tgcttttgtt gccccataat gctctcctgt gcttttggca tgaatccatc aatagtagca
     7861 ggctcgcagg attggttttg tttgcagccg tcaccccccc tctgcaacac gcaataattt
     7921 catcgaggaa tccgttgctt cgggctgttt tcgatagtat tgataacatc atcatcatta
     7981 ttttattgag gccagacatg tccatccgtt ttgcccgcaa agccgactgt gctgccattg
     8041 cggaaattta taaccacgcc gtgttgtata cggcggctat ctggaatgac caaacggtgg
     8101 atgcagataa ccgcattgcc tggtttgaag cgcggaccat agcaggttat ccggtgctgg
     8161 taagtgaaga agatggcgta gtgacgggat atgcctcatt tggcgactgg cgtagtttcg
     8221 atggttttcg ccataccgtg gaacattcgg tttatgtcca tcccgatcat cagggcaaag
     8281 gtctggggcg taaattgtta agccgattga ttgatgaagc gcgagattgc gggaagcatg
     8341 tcatggtcgc cgggatcgaa tcgcaaaatc aggcctcgct gcatctgcac cagtcgctgg
     8401 gatttgtcgt caccgcacaa atgccgcagg tgggcactaa atttgggcgc tggctggatc
     8461 tgacatttat gcagttgcaa ctcgacgaac gcactgaacc ggacgcgatt ggatgaatca
     8521 gtcgctcacc cttgcctttt tgattgccgc cggaattggt ctggtagtcc agaactcgct
     8581 gatggtgcgc atcacccaga cctcctctac cattctcatc gccatgttgc tgaactcact
     8641 ggtggggatt gtgctctttg tttctatttt atggtttaaa caaggtatgg cggggtttgg
     8701 cgaactggtg tccagcgtgc gctggtggac acttatacct ggcttgctgg gatcgttttt
     8761 tgtcttcgcc agtatcagcg gatatcagaa tgtgggagcg gcgacaacta ttgcagtact
     8821 ggtcgccagc cagctgattg gcggtctggt tctggatatc ttccgcagtc atggcgtacc
     8881 actacgggcg ttgttcggcc caatttgtgg cgcaatttta cttgtggttg gcgcctggtt
     8941 ggtagccaga cgctcatttt aatcacagga tggtgccgcc tttggttaac tgttcatggc
     9001 gcgcttccat atcttcttta tgcttgcgac cgtgatgagc aatagcggtg cgcaaacgct
     9061 gctgctgggt gtagcgatct tcccgactta actccgcgtc atcgctcagg gcgattaata
     9121 attcgttcat atgggcaatg acgctctctt caatcgcggc atccacgcgc gcgatgactt
     9181 catccagatg cgacatactt tctccttagc cacttgtgaa atttatcgat tcatccaaat
     9241 gatataagtc tgcgtggtgc cgtcaagcca gtgagtaaga tttaaaacag tgaaaacaac
     9301 agcgccaccg ggaaactcat cggccatgtt gcccccacca gaaaggcgct taagaaacgg
     9361 atgcgtttct tatcgtgaga aagaaaccaa gtgataagaa atgcgattgc tgccataacc
     9421 gcataaaaaa caagcatgtg ttgatagaga gacatgtacg ggcgtccagc cagatgaata
     9481 aaacgcgcgc aaatatgcgt cattttttga cgtgcatcaa gttttttaac cggtttgaga
     9541 aaaaaacatc aaacagcaac ttgcccttat ccatcttcag aatgagtatt attgcggatt
     9601 ttcttaaccc gtactataca cataggggca atgataaaag gtggcaaaaa tgaatgtttc
     9661 cagtagaact gtagtactga taaatgtctt tgctgctgtt ggtttgttta ctcttatctc
     9721 tatgagattt ggctggttta tttgatgtag tgattttgca cgcagcacgt actgtcagag
     9781 aaatcagtgc tctgacagta cgcaagatca tctcatactc ttacgatctg caatattatc
     9841 cttaatgttt aaccatgacg tggcggacga cggtgtaatc ctccagccca taaagtgaca
     9901 tatccttgcc gtaaccagaa agtttctgcc caccgtgcgg catttcactt accagcatga
     9961 aatgggtatt gacccaggta caaccatatt gcagccgtgc actgacgcga tgcgccctgc
    10021 ccacatcttt cgtccatacc gaagatgcaa gtccgtactg gctgtcattc gcccagttta
    10081 ccacctgttc ttcgttgtcg aagagcgtaa cactcactac tggaccaaat acctcttttt
    10141 gcacgatggc atcgtcctgt aatgcgccag ccagcagcgt cggcgcatag taatagccat
    10201 tacccttgcg cttttcaccg ccagtgatca ctttgatgtg ccctgtcgct ttcgcctctt
    10261 ctactgcctt gctgacgcgt tcgagatgcg ccagcgagct taaaggtcca agctccgtag
    10321 actcgtcatc cggcgcacca gattttaacg ttgccactgc agcaccgagt ttttccacca
    10381 gcgtatcgta aatgcctttt tgcgcgtaga tccgacaagc cacagtacaa tcctgtccgg
    10441 cattgtaata gccaaatgta cgtacacctt cgaccactgc ttcaatatcc gcatcatcaa
    10501 aaacaatcac tggcgctttg ccaccaagtt ccatatgagt acgcttaatg gagggcgcgg
    10561 tatggctgat gatgtgctcg ccggtggcga tagagcccgt cagcgacacc atccgcactt
    10621 tgggatgacc ggtcagcgga tcgcccaccg ttttgcctct gccaaacagt acgttaatca
    10681 cgcctgccgg gaagatatct ttcgccagct ctgccaactt caacgcggtc agcggggtaa
    10741 tttctgatgg tttaagcact acgcagttcc ctgccgccag cgccggagcc agtttccacg
    10801 ctgccatcat cagcggataa ttccacggtg cgatagaagc cacgaccccc aacggatcgc
    10861 gacggatcat cgaagtatga ccttcaagat attcacctgc cgccagacca ttcagacagc
    10921 gcgccgcacc cgcgaaaaag cgaaaaacat cgacaatcgc cgggatttca tcattgaacg
    10981 cactatgcag cggtttgcca caattacggg actccagttc ggcaaaaacc tgaccatttt
    11041 cttcgataac atcagccagt ttcagcagac attccgcacg cactttcggc gtggtttgcc
    11101 cccattcggc aaatgctgca tctgccgcgc gcacagcagc atcgacctgc tctgcggatg
    11161 cctcggcaat ctccagtaaa acgtcccccg ttgccggatt atagacaggc tgtttttccc
    11221 cttcgccgct aaccagttct ccgttaatca gtaacttatg ttgcatagca tattcctgta
    11281 tcagtggtta ttgaccattg tcgccttcgc gggttagcca ccaggctccc aggatcggca
    11341 aggttgttac caacataacc agcagtgcca ccacgttagt taccggtaca tcacgcggtc
    11401 gcccaagctg attgagcaac cacaacggta acgttcgttc atgacctgcc gtaaaggtcg
    11461 taacgatgat ttcatcgaac gacaaggcaa acgccagcat tcctcctgcc agtaacgccg
    11521 aactgagatt cggcaacact acgtagcgga aggtttgcca gccattggcc ccaagatcca
    11581 ttgacgcctc aaccagactc catgaggtgc ggcgaaaacg ggcgatgaca ttgttaaaca
    11641 ccacaactac acaaaaagtc gcatgaccga ccacgatggt gaaaaatccc gactccagat
    11701 tgatggtttt aaaggcggtt aataacgcca gaccagtgac aatgcccggc agcgcaatgg
    11761 gcagcagcag taacagcgaa atggcgtttt tgccgaaaaa gtctcgtcgc cacagcgcgg
    11821 cagctgccag cgtccctaac accagcgcca ttaatgtcgc cagcgccgcc actttaagtg
    11881 acagtgtcac ggcatcaaga atatcactac gctgtgctgc cacgctaaac cagcgcagcg
    11941 tcaggccctg cggtggaaaa ctaaacgccg catcttcagt gttaaacgca taggcggcga
    12001 ttatcaggat gggaaaatgt aggaaaacaa cgccgcccca ggccgccagt ttgaggaaaa
    12061 acggtgcgcg ttcagagtgc atcaaacgct cccagacgtt tcacgaacgc caggtacagt
    12121 gcgataagaa caatcggcac cagggtgaat gccgccgcca tcggcatatt gccaatcgcc
    12181 ccctgctggg aataaaccat attgccgata aaatatcctg gcgggccaac cagctgcggg
    12241 acgataaaat cgcccagtgt gagtgagaag gtaaagatag agccagcggc aatacccggg
    12301 atcgccagcg gcaacaccac atagcgaaag gtttgtcgtg gacgtgcgcc gagatcagct
    12361 gacgcctgca acaatgacgg cggcaaacgc tcaagcgccg cctgcacggg caggatcatg
    12421 aacggcagcc agatatagag aaacaccaga aagcgcccca gcccggaagt tgacagcgta
    12481 ttgccgccca ccgcaggtaa tgtaaggaac gctgtcagca gtggttccag cccaagatgt
    12541 tgtaaaaacc actgagccac gccatctttt gccagcaata acgtccaggc ataggcttta
    12601 acaatgtagc tcgcccacat cggcaacatt accgcaatat aaaaaaacgc tttcattttc
    12661 ccgctggtat agcgcgccat ataccacgcc attggaaaag ccagaatggc gctggcgata
    12721 gtgactgcca cagccatagt cagcgtgcgg agaatgatgt cgtaattcgc cggattaaac
    12781 agcgcacgga tgttcgccag cgtcagttcc ggcgttaccg acatggtgaa atcgtcaaag
    12841 gtataaaagc cctgccataa cagcgttagc agcgagccga aatagacaat gccaaaccac
    12901 attagcgggc caagcagcag caaaaacagc cccagccccg gattccgcca gaagaaaccg
    12961 gacaccttac ccagacctgg acgtgaaggt gattgcaata cgttcatcgc cattcacctc
    13021 tcctcaacca gcggcaccat cacatcacgc gaccaggaaa ccatcacctg ttgtccgggc
    13081 gtgagcgtgg caggcagttc ttcgcctgtc atattggcct gactcaccag cagtttttca
    13141 ccgccgttca atttcagttc aaaacgagtt gccgcgccct gatattgcac cgcctggatc
    13201 gtgccattgg cctgcagttc accaggggtg ttgaggcgga tatgttccgg ccgcagggcg
    13261 aagcttcccg tcatgccaca aagtttctct gccatcagtc catcaaaaac attcgatgta
    13321 ccaacgaacc cggcaacaaa cggcgtgcgc gggcgcatat agagatcgcg cggggaatcg
    13381 acctgctcaa tgcgtccatt attgaaaacc gccacacgat cggacatcga taacgcttcg
    13441 ccctgatcgt gggtaacgca gataaaagtg ataccgagag actgttgcag ttttttcagc
    13501 tcaagctgca tctgctcacg caatttgaga tccagtgcgc cgagcggttc atccaacagc
    13561 aatacgcgcg gttcattcac caatgctctg gcgatagcaa cccgctggcg ctgaccacca
    13621 gaaagttgtg acggtttacg ttgctgtaca aaccccaacg ccactttctc cagcgcctct
    13681 tgcgccattg cgtgccgctg ctttttattc acgcctttga ccatcagccc ataggcgaca
    13741 ttgtcaagaa tcgacatatg cggaaatagc gcgtagtcct gaaagacagt attcacgtcc
    13801 cgctcccacg gtggcagatt gctggcgggt ttaccaaaga tagagatagc cccgccggaa
    13861 agctgttcga agccagcaat caggcgcagg caggtggttt tgccggagcc ggacggcccc
    13921 agcatagaga agaactcacc atcttttatc gcaatactga cgccatctac tgcgcgcacg
    13981 tcaccgtaca accgcgagac gttgtcaaac tccactgcgt acgtcataaa gcaccccagc
    14041 gaagttagcg accgcccata atggcaatgt aatcctgcgt ccagcgactg tagggaacaa
    14101 acttgccccc ttctgctata ggcgttttcc agaaggctat tttatcgaaa tagttaaaac
    14161 cgtttgtttc acaacctttc tcgcctaata acgaactggc tttacaccct tccggcacga
    14221 ctggtaacga gccaaaccag gccgccacat cgccctgcac ttttggcgtt aatgaccagt
    14281 tcatccattt gtaggcgcaa accggatgtt tcgcttcgct atgtagcatg gtggtgtcag
    14341 cccaaccggt aacgccctcc ttcgggaaaa cggtagcgac aggctgacct tcggctttca
    14401 gggcgttggt ctgatagggc caggcactgg aagcgaccac accctcgttt ttgaaatcgc
    14461 tcatttgcac ggtagtgtca tgccagtagc gatggatcaa actatgttga tcacgcagca
    14521 ctttcagcac cgcctggtac tgttcttcgg tgagttgata cggatcgctg atgcccaact
    14581 gcggctgagt ggctttaacg aacaacgcag cgtccgcaat gtagataggg ccatcataag
    14641 cctgaacgcg gcctttattg ctcttgccgt cctgcagatt ttgctcaaca aaaaccactt
    14701 gccagctatc cggcggcgtc gggaaggttt tagtgttgta catcagcagg ttcggccccc
    14761 attggtaagg tgtgccgtaa actttgccgc caacgttaaa ccagtcgcct ttaaccacgc
    14821 gcggatcaag cgttttccag ttgggaatca aggcggtatt aatcggctgc acgcgtttac
    14881 ccataatcaa acgcagcgag gcatcgccgg atgccgtaac cagatcgtaa ccccctttgg
    14941 tcatcagact gaccatctca tcggaagtcg cggcggtttt aacattcacc gcgcagcctg
    15001 tctctttttc gaattgcgtt acccagtcgt attgtttatc agtttgtccg cgttcgatat
    15061 atcccggcca ggcgataata tccagtcgcc cttccggttt atctaaattg gtaggcggtt
    15121 cggcggcgtg agcggtcatt attgtcatgc tgagcgcaca caggctgctg cgggcaaatg
    15181 tcttgctcat aaggtcttac tcctgtcgta attaattgtt cagccgccgt gccgtaaaaa
    15241 tatctccttg tttaaccata gacggcggct tgcaggcaca ccttcgcccc tgagaaaatt
    15301 taatcaggtt gtgagctacc gcgcattatg ccgtctgtaa ggttatcttc tggagtatag
    15361 acaaggagtt aggaaatatg actgctattc gaaattcata ttacccaatc taagaggaat
    15421 taaattaaac gctattacag ccgttcttga ataagtttgc ctaattgttt taccgcctgt
    15481 tcttcacgtt ctccccacag ccacgcggta ttaaaacgaa aaaaacgtga ccagttttca
    15541 ccggtagaaa acattttacc tggcgcaata ctgatatgat gcgtcagtgc cgccaggctc
    15601 aattcgccgg catctaacgg ctcggggagc tccagccaga gaaaataacc gctgtcatta
    15661 tgatgaattt tcacttccgc aggcagataa cgcagcagtg cctgccaggc acgttgttta
    15721 cgttccgcaa gctggcgacg caggcgacga agatgggcgt cgtatcggcg cgtggaaagg
    15781 taatccacca gcgcaagctg catcggtgag ctggtggaaa gcgtactcat cagctgcaag
    15841 cgttgaattt tacgtgcatg ttttccggcg gcgacccaac caatacgaaa accaggcacc
    15901 agacatttcg aaaacgaaga gcaatgcaaa acgccatcgt ggcgatccca cgctttcgca
    15961 ggcagcggtt tttcccgtcc aaaataaagt tcgctgtaaa cgtcatcttc aatcagcgtt
    16021 acgttgtact gattgagcaa cgccaccagt tgtgcttttt tttgcggcgt taaggtaaat
    16081 ccgagtggat tctggctatt agtcatcagc cagcacgctt tcaccggata ctcctgcaac
    16141 gccagttcca gcgcctgaag atcgatccct tctttaacat ccgtcgccac cgataacgcc
    16201 ttcagccgta gccgctccag cgcctgcaac gcaccgtaga aacaaggatt ctctactatc
    16261 acccaatcgc ccggttcagt taccgcttgc aaactgaggt ttaatgcctc taacgccccg
    16321 gcagtgatga caatttcatc aggagaaatg gtgatcccct gtaaggcata gcgacgggca
    16381 atagcctgac gcagttctgc gtttcctggc ggcaagtttt caatcacgct catcgccgtg
    16441 gcggttttgc ttacctgcgc cagcgagcgg tttagttgtt ggagggggaa aagtcgcggg
    16501 tcgggaaagg ccgaggcaaa cggaacgacc gacggatcgc gactggcctg caacatatca
    16561 aaaatataag tgttgatatc gactgcttca tctcgagtga ctggaatgac tggcactttc
    16621 ggcattttta ttgcctgtgg cgcaacgtaa taacccgact gcggtcgtgc gataatatat
    16681 ccctgacttt cgagcaactg ataggcatgg ctgacagtca taaagctcat gccagaaagc
    16741 gccacctggt cacgcaacga aggtaaacga tcgccgggtt gccagatacc cgacgcaatc
    16801 tgctcgcgta attgttctgc aagctgctgg tattttttca ttatcgaatc gtaattatgt
    16861 gcagatgatt cggcagtcta tatcagttgt taaaaatgac aacttttcgt taactgtaat
    16921 taaaccatca ccagcgataa ctctttgcca agcgcctttg ccgcgagctg gacggcgtcg
    16981 atttttgtcg catgatgcaa gttaaatagg cgagtaatct cttgtttagg tttgccaatt
    17041 cgcctggcta actcttgctg agtgatttct gactgtaaaa aagcatttaa cagcaatacc
    17101 ttagaggcga cgctcaaagg tacttcaata aagtgatcat gactatttaa tggcgaaggt
    17161 aaagggataa gctcgttatc ttcaaaataa aaatcaaatg cggtcagtaa agcatctttt
    17221 gccgcttcca tcgcttcagc gacagtttcg ccctgcgtca acgcttcagg gatatccaca
    17281 aaagaaacca tataaccgcc ttccggcgcg ggtgtaagag tgacgggata acgcataatt
    17341 tcgactgtct ctcgcatgtc attaaccttt ttaattggcg attaactcaa accgagttgt
    17401 ttcaggattg ctttacgcaa tggttcttta atctcatcgc aggggtgacg cggcatgaca
    17461 ctgcgcctcc catgaaacct gagtttcaaa tggttgctgc cattcgctac atcgacgccc
    17521 tgagattcga gccaacgtct gaactcgctt tgtttcacat cgcctccctg cggctgagta
    17581 tatcaacaaa gtaaacatta gtgtttacct ccgtgtgtaa tttgctcgaa aatagcacta
    17641 aatcgccgca acggcagaat gcaatgcaaa atcaggaagg aagattccag aatttctttg
    17701 cgctaatttg caaaactgaa taataacctc aagaaacgat cggcatcata gcggcaaaag
    17761 gtaagaaaaa ggtggcagga tgtttacgaa ggcgttatcg gttgtcttat taacgtgtgc
    17821 gctgttctca ggacaactca tggcagggca caaaggacat gaatttgtgt gggtaaagaa
    17881 tgtggatcat cagctgcgtc atgaagcgga cagcgatgaa ttgcgtgctg tggcggaaga
    17941 gtcggcggaa ggtttgcgcg agcattttta ctggcaaaaa tcgcgcaaac cagaagcggg
    18001 acaacgttga gtcgtttgat gccggatgcg gcgtaacgcc ttacccggca ggcaatatca
    18061 tgcagattca ataagttgtg agtaaccaga aaactggcgt tttacccttt tcgcttcggc
    18121 aatgttttca gcagctcatc cggacttacg gatgccacta cgcttcccgg taacggcatt
    18181 ttcagtatgt gatttttgat tttgccaatg acgtgcatct cgcatgggcg gcaatcaaac
    18241 tttagcgtta atacttcatc gccgttaacc agttgcattg gcgtcgcctt ccagcttttg
    18301 atattgcctt tcgcctgttt cgggcacaga ttaaaggcaa agcgcagaca atgcttggtg
    18361 atcatcaccg ggacttcgcc cttctcttca tgtgcctcat acgccgcgtc aatcagctgc
    18421 acaccatagc gatgataaaa ttcacgcgct ttctggttgt aaacgttcgc gaggaaactc
    18481 agatgtgttt gcggataaac cggcgcagga tcagcaaccg gtttacggct gccgcgctgg
    18541 taactggcaa gacgcgcagc atccagcatg tcggcagctt cacggcggaa ctggtttaac
    18601 agactgtttg gtacaaacag cgcccccggc aaattaattt gcacatcgcg ggcgtaatag
    18661 agggtttgcc ccagttttgc cagaccatcc ttcagattgt tcattgcttt ttctgcgtta
    18721 ttggcttcgt cgaactgccc atccagcgta tgcgttatgc tgaccccctc ttcactggtg
    18781 agagtcagaa tcaattgttc ctgccaaccg cccagttcaa tgtctaccgc cacccgacgt
    18841 tcgctagagg tttttgtcag tgcctgctgc cagttatgat caagattacg gtttagtggg
    18901 tgatgcgggc gaattttgtg caaatctgct ggcatttcat tgggccagac gcggtactga
    18961 ttttctccgg ttttctcgac cgtattggca cgaaaaccga cgacttcacg tttaatcaac
    19021 acgttcaggc catcgccatt tgccagtggc tcggtaacgg caacatcgag atgatctttc
    19081 gccactttca ctacttcacc taccggcagg ccgataaatt tcggcgaatc gaacgcgcca
    19141 atatcgcctt tacgggcatt cacaaaataa tctgtgctac cacggtggaa agtcttttcc
    19201 gtcgatggaa caaagaaatg ttcagtacga cctgatgaag cgcgcgccag atcgccacgt
    19261 tcttcaataa tggcatcaag catctggcga taatgggcgg tgatattctt cacgtagctc
    19321 atatctttgt aacgcccttc aatcttgaag gaacgtacgc cagcatcaat cagcgcgccg
    19381 aggttggcag tctgatcgtt atctttcatc gacagcagat gtttttcata ggaaaccacc
    19441 cgcccctgat cgtctttcaa tgtgtatggc aaacggcacg cttgcgagca atcgccacgg
    19501 ttggcgctac gccctgtttg cgcatgagaa atgtagcact gacccgaata ggccacgcac
    19561 agtgcgccat gaataaagaa ttcaatggtc gcgtccgtgg cctggtgaat cgcgcggatc
    19621 tgatcaagat tcagctctcg cgccagcaca atctgcgtga agccaacatc agagaggaac
    19681 ttcgcttttt ctactgtacg aatgtcgcac tgcgtactgg cgtgcagttc aatcggcgga
    19741 atatcaagtt ccagaatccc catatcctga acaatcagcg catcgacacc ggtctggtag
    19801 aggtcagtaa tcagccgttg cgcgggttcc agctcatcat catgcaaaat ggtgttaagc
    19861 gtgacgaaaa tttttgcacc ataacgatgg gcaaacggca ccagctcggc aatatctttc
    19921 aggctattac tggcattatg acgggcacca aaaccagggc cgccgatata aacagcatcg
    19981 gcaccgtgca aaatagcttc gcgggcaatg gcggcatcgc gtgccgggct taacagttca
    20041 agtcgatgag aagatacggt cacattctta tccagtagct gaaaaatggc ggctatttta
    20101 gccacaagcg cgacgttgcg aaatagtttt cccgcttaac tgcgcgggta atggatgagg
    20161 gaatgaaaat gtacggtttg ctccccacca ttacgataga tatgcgtgac gtctgcagca
    20221 aatcgaacgc cttccccaca attaagggtc tgccattcac catcaacgca cagatcaagt
    20281 tgtccatcaa tgacgacgac atgttcgatt acccccttct catggggcgt cgattcgctt
    20341 attgcaccgg gtgccatctg aatcgagaag tgttcaaagc agagttgtgg atcataagga
    20401 aacagcgaaa tgatcaccat cgcctgctgt tgtggatcat aaactgaagg cgtagcggac
    20461 tgtggcggag aaataaatgt cgaaaatggc acgttcaacc cagtggcaat tttccataat
    20521 gtcgcgaccg tcgggctgga ctcattacgc tcaatctgtc ctaacattgc tttagaaacg
    20581 cccgtcgctt ctgccagtcg cgaaagactc cagccgcgct gctgacgtag ttgtttaagt
    20641 gtggtggata aaaagcgagc gagattttcc atgcgactcc tccggcaaaa cggaagttta
    20701 tcacttgtgc gttataacgg acaaatgcta cggtgcctgt acgctataac gcacgaggtg
    20761 actatgcgtc tgttttctgt tcctccaccc acgctactgg cggggtttct ggcggtatta
    20821 attggctacg ccagttcagc ggcaataatc tggcaagcag cgattgtcgc cggagccacc
    20881 actgcacaaa tctctggctg gatgacggcg ctggggctgg caatgggcgt cagtacgctg
    20941 actctgacat tatggtatcg cgtacctgtt ctcaccgcat ggtcaacgcc tggcgcggct
    21001 ttgttggtca ccggattgca gggactaaca cttaacgaag ccatcggcgt ttttattgtc
    21061 accaacgcgt taatagtcct ctgcggcata acgggactct ttgctcgtct gatgcgcatt
    21121 attccgcact cgcttgcggc agcaatgctt tccgggattt tattacgctt tggtttacag
    21181 gcgtttgcca gtctggacgg tcaatttacg ttgtgtggaa gtatgttgct ggtatggctg
    21241 gcaaccaagg ccgttgcgcc gcgctatgcg gtaattgccg cgatgattat tgggatcgtg
    21301 atcgttatcg cgcaaggtga cgttgtcaca actgatgttg tctttaaacc cgttctcccc
    21361 acttatatta cccctgattt ttcgtttgct cacagcctga gcgttgcact cccccctttt
    21421 tctggtgacg atggcatcgc aaaacgcacc gggtatcgca gcaatgaaag cagccgggta
    21481 ttcggctcct gtttcgccgt tgattgtgtt tactggattg ctggcactgg ttttttcccc
    21541 tttcggcgtt tattccgtcg gtattgcggc aatcaccgcg gctatttgcc aaagcccgga
    21601 agcgcatccg gataaagatc aacgttggct ggcagctgcc gttgcaggca ttttctattt
    21661 gctcgcaggt ctgtttggta gtgccattac cgggatgatg gctgccctgc ccgtaagttg
    21721 gatccagatg ctggcaggtc tggcgctgtt gagtaccatt ggcggcagtt tgtatcaggc
    21781 gctgcataat gagcgtgagc gcgacgcggc ggtggtggca tttctggtaa cggcaagtgg
    21841 attgacgctg gtcgggattg gttctgcgtt ttggggatta attgccggag gcgtttgtta
    21901 cgtggtgttg aatttaatcg ctgacagaaa ccgataataa aaagcgccat ctaacgatgg
    21961 cgcgtctgga actacttttt gttaacgtca aacatggtgg catcggtcgc catgtcatca
    22021 ataacttgtt taatattttc gaaagccatt ggcgtacttt cgttattcag gtctttacct
    22081 tcgccctgac gaacgacttt gataaccggt ttattagtcg ctgcatcaat cagctcacct
    22141 tcaaaataca agcgagtatc catggtacgg tggcctgtag ccatttgcgt ccccgcaacc
    22201 actaatgcaa caggaaccac ttcatagaat tgcagccctt ctttgctggt atctacaccg
    22261 gtaatggccc cacggaaaat cagactacgc ggccccgcgg tggtaaccag tggtttacgc
    22321 tgcgctatcg cttctttcat ttcggtgttg gtatagttca aaattttatc cagaactttc
    22381 tgccctacct gggtcgaagg tttcggtacc ggataataag tgattgggtt ccagacgatg
    22441 ctgtcatatt tgctttgatc aaaactcggg tctacccaac gtaaaaccgg tttacctgta
    22501 gccgaggttg tttcttttaa atcagagtaa ttgtttaaaa aaccagaata tttatctggc
    22561 tgggtgattt tagaggcaca accagacaga gccaacaagc cgcataaagc tgcaactttc
    22621 gcaaatgatg tggtacgcat gaatagtttc ctgagatgat tatattactt tttaagttat
    22681 agcaaaaagc gccgcttcgt gttgtggcaa aaaaagtgtt ggtgaaaata aattttttta
    22741 gccccttttc agacgacata gtcaatgccg caaaaggggc taagtcaatc atcagtagtt
    22801 aaatttcagt aagttaagct gctaaaaaac ttcgactact gtgattaatt tcacatccct
    22861 tcgataaaaa atatgcttac cgaaaagaag gacagtaaac catctcagcg tggggaattt
    22921 tcacgcgctt tgttcttacc gggtcatttt ttacgtgcca gcatcgtggc gaaacgcagt
    22981 ttaatacgat taccgttggc gtcggtgcgg tgcagctcgc cgacgtcttc attgtatttc
    23041 accatctccc agccttcgta atatcgacgt aattctccct ctttgaaggc aaacgggaag
    23101 ccgacggtac atggataatc agcggtatcc atcgccgcca caatcaggtt gtaaccgcca
    23161 ggtttagtgc aacgttgcat attggcaatc agccctggga tggttttagc ctcaaggaac
    23221 atcagcacca cagtcgaaag aataaaatcg tactctccat caaatgtgag gttattcaga
    23281 tcaacgaccc gggtgtgtaa attatccaga ttttcaatgg atttaatgcg ctcgacgttg
    23341 gcgatactca tggcattttt atcccatgcg tcaacatcat aaccattggc tgccaggtaa
    23401 agactgttac gaccattgcc acagcccaga tccagcgttt tacccggttt aaccactttc
    23461 accgcttcca gtacttcaga gtgtgtgcgg gttaattcat atttatcagt aaaataattt
    23521 tcgtcacgaa tgatcattct ttgtcctctg ctttcattaa aacggcgcgc tcggttctga
    23581 ctaacaattt tccctgcatc agaagcgcca aagtacggat gagcagtatt gcaataataa
    23641 aattggtaaa gataaacagc ggcaccgcca gcgtatggaa aaatccatta tcgctgccac
    23701 tccccagatg caaaccggtg gttgccagtg cagatacgcc gaacgagaaa ctccagaatg
    23761 aagcattaaa tggctgggag agataccatg gcatcagacg tagcataaac agcagttgca
    23821 gcagtccata accgaaaagc attttcgcca gcgtgtcacc ctcgccgccg ttgacgctca
    23881 gccaggcact acaggccacc agcgcaggag cgagctgaat gccgagtgat gtccgcagtg
    23941 ccgtgggtaa ttctcccgaa ctacgcagac gctgcaagat caccggttca aggcttagcc
    24001 atgagaaaac gcctgcgcct aaaaacacca gaccggcgtc ggtgtagccc aacgcaccac
    24061 aggccattgc gctgataaag ttgttggcaa ctgtcggcag atacagtcca ggcgtagtag
    24121 cttcttcagg gtgagatccg cgccataatc ccgcagtttg ccaggcggca taagccaact
    24181 gaaccacgac accaaaactg aacaggcaca ccgccagtgg gcgaaaccac ggaacaaaac
    24241 caatcgccac cagcatcgtc gttgcaggaa acaaactcac aaaactgctc agcactggat
    24301 ggcgaacttc cgccagcacg ctatgcggaa agcgtatgag tcgggtaata aatgcgctag
    24361 tcaataatcc ccagatgatc atcgccagaa tcaccagccc atcccctaac cagtggctga
    24421 cctgccaaac ctggctggca tagcgccagg caaatcccat ccctatcgtc cccaacacaa
    24481 taccaaagta gcctgccggc aaattgagca ctttatcgct ctgcatcttc ttactcattt
    24541 tgtttaattt ataaagtata tttgaaatgc attttatgga attttctcga gcatagccag
    24601 agccgcagaa tttgctacgg ttatagtaag tcacctgtgg gaaaacgcta tgcgcaaata
    24661 tcgtctaagt gaagaacagc gagcctttag ttatcaggaa gatggcacta aaaaaaatgt
    24721 gttattacgg cagattatcg ccattagcga ttttaacgat gtgatagcag gaaccgctgg
    24781 cggctggatc gatcgcgaaa cggtgttagc acaggaagga aattgctgga tttacgacca
    24841 gaatgctatt gcgttcggcg gaacggtgat ttccggtaat acgcgcatca ccggcaccag
    24901 cgtactgtgg ggagaggttt acgcaacgga taatgtctgg atcgataata gtgaaatcaa
    24961 ccagggtgca tatataagtg acagtgtcac cattcatgac tcactggttt gcgggcaatg
    25021 tagaatattt ggtcacgcct taattgatca acattctatg atcgtcgccg cacaaggctt
    25081 aacgcccgac catcaactcc tgctccaaat ttatgatcgc gcaagggtta gcgcctcgcg
    25141 tattgtccac caggcacaaa tttatggcga tgccgtcatc cggtatgctt ttattgaaca
    25201 tcgcgccgaa gtttttgatt ttgccagtat tgaaggtaat gaagagaaca acgtctggct
    25261 atgcgattgc gctaaagtct acggtcacgc gcaggtgaaa gctggcatag aagaagatgc
    25321 cattccaacg attcattaca gttcgcaggt ggcggaatat gccattgtgg aaggtaattg
    25381 tgtgttaaaa catcatgtct tgattggcgg aaatgccgtg gtacgtggcg gaccgattct
    25441 gctcgacgag cacgttgtaa tacaaggtga gagccgtatt acaggtgcgg tgataattga
    25501 aaaccatgtg gagttaaccg accacgccgt cgttgaagcc tttgatggcg acacggttca
    25561 tgtccgtggt ccaaaagtca ttaacggcga ggagcgcatt acgcgaacgc cgctggcagg
    25621 gttattatga atcgataata cgcgcgtata agttcacatc atcataggca tcattcagga
    25681 actcagcctg tttcaggcaa ccttcaagga taaaaccatt gcgcaaagcg acctggttgc
    25741 tttgcggatt gtccacccga catttgatca cgaagcgtct aagttcacca gactgagcgt
    25801 aatgatgaat cagtgcctgc agcgcctgag aaatgatccc ctgcccctga tgagattcgt
    25861 ccagtcagta gccgatttca gcggttttat tcagtggttc aatacgatta aacgagataa
    25921 cgccgataag ttcatcttct ttgaaaatca tgaacatttt ggcatagccg cgttgatgca
    25981 acatcacatt accctgcacc gtttttcgcg tgtcctcttc aatctgaacg aactgcggcc
    26041 agtttagcga ctgctgtaac caggttttat ttttacagat taactgataa agaggagtga
    26101 cgtgattttc tgcaacagca tgtaattcaa gtgattcgct tacttttatc gtttcagtca
    26161 tcttatctcc agtcaatcca tgcagttgtt aaaaaagccg cctggctggc ggcttaggaa
    26221 agtttacacc tctttgacgc tctcatgctg caggattttg agcatctcat cttttaactg
    26281 tagcttttgt tttttcatgc ggaccacttc cgcattgtac cctcgatcgt cggaaccttc
    26341 ctttctggca atttcatgat caagtttatt gtgtttatcg aacaaggaca taaagcgagg
    26401 attttcgttt ttcagacggg atattaaatc tcgatattct ggaaacatac gcactccctg
    26461 ttagtggctg acaaggtgta tctgaaatac acaccatcag attactcatt taacttaggg
    26521 aaaaatgcga gcgggatcgt gtttaaccag acggcacctc ccgaagaagg tgccgaagaa
    26581 aaaacgatta actcatcacg cggtcgtcaa catactgacg tttatccggc gctggcggga
    26641 aatactgata cagccatgtt tcactgatag cgtcgccctg acaacgcaga tacatccgca
    26701 tatcgaccgg atcagtggag tccgaagtcg gataccagtc aaactgaata cgataaccat
    26761 caatgggttc aatatagaga atttcgattt gcttcgcttc cccactggaa agcgtaatca
    26821 ccggctcaat gccttttggt gcggcagctt tcagatcacc accaacgaaa tcgacggcaa
    26881 aacgacgcgc ccatttttcg ggatagtgtt cacctggtgc ccaaccttcc gggaaaccgc
    26941 ccatgccggt acgcgtcgcc ataacgcgcg ctaatgggca atgaacaggc ggttgcgcac
    27001 tccagtacag acgatactgg aatgcaaact catcacccgc ctttacagct ttttctggct
    27061 gccagaagca gacaatgtta tccagcgttt cgcccgttgt tgggatttcc atcaggccga
    27121 tggtgccctt accccacttg ttacgcggtt ccacccacag acttgggcgt ttgttatacc
    27181 agcccataat gtcctgataa tgggagaaat cacgatccag ttgcaataaa ccaaaccctt
    27241 tcgggttgtt gtcggtgtaa gcattgaact gcaatttttg cggattattc agcggacggc
    27301 aaatccactc gccgttgccc cgccacatgg acagacgatc agagtcatga atttgcggat
    27361 gaatggtgtc gcacatccga cgttcattag taccgcagct gaacatactg gtcatcggcg
    27421 caatgcccag ctgtttaatg tctttgcgcg catacaggtg attttccaca tccataatca
    27481 cctgactttt ctcacaatgg atagtgaact tataggcacc agtaatgctg gcgctatcga
    27541 gcaacgcata aacggtaaag gtagttgccc ccggttttac cgtatcaaac cagaaggcgg
    27601 taaagtcggg gaactcttct ttactgtcgg tgtaagtgtc aatcgccagg ccgcgggccg
    27661 acaaaccgta ttgatatgtg tcatcaacgg cgcggaaata actcgctccg aggaatgata
    27721 ctacatcacg gcgcgccagt tcgggggctt taaacacgcg aaaaccggca aagccgagat
    27781 cgctttgccc ttctaattgt ttggtatcaa cacctgcatc gttgtatttg aacaactccg
    27841 ggcgaaagtg aatttcacgc gccagatgtg ttgctggatc tacagaaaac atacgaacgc
    27901 ggcgacggaa tcccattccc atatggaaga actgagcgtc cagttgacgg ttctcaacgt
    27961 tatgccagag cgatttttcg gcgtcgtatt gaatactgtt ataagcctgc ggcgtcattg
    28021 tcgccagcgt gtcaggtaac ggacgaggcg caccacgcca cgctgtttgc gctaagtcgt
    28081 gcgccattga ctgtagaatg gagaagtcaa aacgctgggt ttgcccgtcg gcaatatcag
    28141 aatctgccgc gaatgccgcc tgagaaaaaa gagaagcaat gccgctggta ccgcacacgg
    28201 cggccatagc cattgaacct ttaataaatc gtctacgatc catacctgaa agtgagtcct
    28261 tctggtgttg tgaatggtcc cgcgctggtc gttttcagcg gcaagaaagt gcttatttat
    28321 gaccgctcac tctagacaat atttattaat aatccaattg ttgaactgag agaatatgca
    28381 taaatcggca atttttttat gtcgatggga tgagcctgat tcgtttgtaa aatcaggcaa
    28441 atttttgcaa aggcggatgg tcagaattta aagcaaacca cagcgacgtt tactgcgttc
    28501 ttccgcttcc tgataaagct ggaactcatc caaaaccgga caacccaacg cctgctcaaa
    28561 tgcttcgcga ctggcattac cgtggctgcg tgcctgcgtt tcattaccca gatttgactg
    28621 gaagatcccc gccgcgctaa cgggcaagaa atcttcatag gtaatgggtt gcgccgcgac
    28681 ccaaccacgt tcaattaagg gctgtggatc gtctccggga tgaatcgcct gacgatgcgc
    28741 ctcacctgaa ggcgtcagac ggtaccggaa ccatgccagc ccttgctgac gcattaaaaa
    28801 ctcactgtca ggaaaagtgc ggaaggtttc ctgtaaatgc atttggtgag tgagattatc
    28861 ctgcccggtt ccagcgttac gcagaagatc atcatacagt tgtcgacctt tcggcgttaa
    28921 tgccacgcca cgctgctcaa tttcaccaaa gcgcgcagta tgcgtgccct gtttctgccc
    28981 cgcaaacaac accgtctctt ccagtgcttt aaagctggtc tggcgtagta aaatcggtac
    29041 ctcgcggcgc ggcggccctt cgatcagaat ttttggttca attccgcatt caggcatcat
    29101 cgactgcacc cggtcaatat ccagcgtacg tggcgtcaga tggttgatat ggcatccagg
    29161 aaaacagacc acatcagcaa tcaaccgatg ttcgttgtgc aaggcgcgat aggtttcttc
    29221 atctaccgtt gctgactggt gccaacggaa cgtttccagg gcctcctgca caaactcctg
    29281 tgcctgtgtt tcgttaaaac cgccccgctg gtcatactcc tctaacagtt gtcgacaacg
    29341 tggggtgaag atatcgcgct gacgcagaat ctccgccgct ttctggcgca aaatttcgtt
    29401 ctcgataagc tcaaggcgga gtaaggaggt aaaaacgcgg aagggattac gcgccagaga
    29461 agcatcatca atgggccgaa atgctgtcga atgtaccggc acccctgcct gcgagagatc
    29521 gtaatagctc accgggtaca tccccataat ggcaaacatc cgccgaagag tagcaagctc
    29581 ttgtgcagtc ccaacgcgaa tcgccccatg acgttcaaca ttcagtcgcg ccagctcgtc
    29641 tgcatttacc attttttcgt gcagttgagg attgttttcc agcacagcca gattcacatc
    29701 agctaccagt tccagcagcg tgccatattg cggaacttct tgctggtaca tggctgacat
    29761 tgcctgcgaa aactgttccc gaatctcatc cgccgtgatg ctgttcgcca tagtgtctgc
    29821 ctccagtggt cagtaatctg gagtgtaggt aaccgcattc actctggcgg gaagaattta
    29881 caaactgtga tctcgccgcg aaaacatcaa tattatccat tttgctgtaa cataattgct
    29941 ttaattgtta ataatatttt gcaatcaagt tatcataatc aaacaacttc acttgtcagc
    30001 gacaccgctt cgtttttaac atcgcttatg gaaaaaaata gtctgtttag tcagcgcatc
    30061 cgtttgcgcc accttcatac attcgtagct gtcgcacaac aaggaacttt ggggcgcgcg
    30121 gctgaaaccc ttaatttgag tcaacctgcg ctctctaaga cattaaatga actggagcag
    30181 ctgacgggcg ctcgcttgtt tgagcgtggt cgtcaggggg cgcaacttac cttacccggc
    30241 gaacaatttt taacgcatgc agtcagagta cttgacgcca tcaacactgc cggacagtcg
    30301 cttcatcgta aagaaggtcc taataatgat gtcgtcaggg ttggtgcact acctactgcg
    30361 gcactgggga tattaccttc ggttataggt cagtttcatc agcaacaaaa agagacgacc
    30421 ttgcaagttg cgacaatgag taaccctatg attctggcgg gtttgaaaac cggggaaatc
    30481 gatatcggca ttggtcggat gtcagatcct gaactgatga ccgggcttaa ttacgaactg
    30541 ctgtttcttg aatcgctgaa gctggttgtc cgccctaatc atccgctact tcaggagaac
    30601 gtaacgctaa gccgggtgct ggaatggccg gtcgttgtat caccagaagg cactgcgcca
    30661 cgccagcatt cagatgcatt agtacagagc cagggatgta aaattccttc gggttgtatc
    30721 gaaacgctgt ctgcttcgct atctcgtcaa cttacggttg aatacgacta cgtgtggttt
    30781 gtcccttctg gcgcggtaaa agacgacctg cgtcatgcca cgctggtggc cctgcctgtt
    30841 ccgggacatg gtgcaggcga accgattgga atactgaccc gcgtagatgc gacgttctct
    30901 tctggttgcc agttgatgat taacgctatt cgaaaatcaa tgccgttctg aaaggtgaag
    30961 ggatctgtcg atccctcctt taacattttc acaccggagc gaaactaact agttcacccg
    31021 ctccgcgagg ttctgccaac acagattgtt tgttcagacg gaatacgtcc accgcctcag
    31081 ttaatcgtgc cgcctgttct tcaagggaca ccgccgccgc tgaggcctct tctaccagag
    31141 aagcattctg ttgcgtcacc ttatccattt cagaaatcgc ctggctaacc tgcgttatgc
    31201 ccctactttg ctcatccgag gcggcggcaa tttcctgcat gatatgtgtg acactcgcga
    31261 cggcatcaac aatagtgctc atggttttcc cggccgttgc cacctcatcc gatcccaggt
    31321 caattaacct gactgattca ctgatcaagc cttcaatttc ttttgccgcc tgggcgctgc
    31381 ggcttgcgag tgtccgtact tcgctggcga caacggcaaa tccacgccct tgctcacccg
    31441 ctcgcgcagc ttcaacggca gcattcagtg ccagaatatt cgtctggaaa gcaatactgt
    31501 tgatgacggc ggtgatctca gaaattttct tcgaactcgt ggagatagcg cccatcgttt
    31561 ttactacacc ggaaaccgtc tgcccgccat cgctggcttt aatagaagcc tcttgcgcca
    31621 gtttgctggc ataatgcgcg ttatccgcat tctgtttcac cgtcgcagtg agttgctcca
    31681 tgctggcggc agtttgttcg atagccgccg cttgttcttc ggtgcgagat gacaggtccg
    31741 cattgccagc tgaaatttcg ctggtgccac gataaatctc ttccgcaccc tgtcgaacag
    31801 tccctacggt catccccagt gaatgctgca tttgctgtaa atgacgactt aagcgaccga
    31861 tttcattacg acccgccggt tcatccttca tcgtcagatc gccactagcg attttttcaa
    31921 tccgttgtgc ggcatgttgc agtggacgaa tgacgatccg acgtagcacc ataaatgtta
    31981 tcagcgtcat gaccagggca agcacaaacg caccaatcat gaacatcccg cccaggcggg
    32041 tgcgctgatg ggccagttcc gccagttggt tggctctggt gctacgtatc ttaacggctt
    32101 tgttcaaaat atcggtataa gcattatcca gcgttcggat ctgctcactt tcatgattga
    32161 taatcgcttc aaacatgcca tttttggcat atttcaacat aggttgcata cccgtgatat
    32221 aagcctgaaa gcgttgattt aattcagtgt cgagggcttc atcagcaggt gttttcaccg
    32281 gccgattctg ataagcacga taaccttgct gcgactgttt aatctccgat tcggcttgcg
    32341 caatatttcg cttcattgct tccatttccg caatacgact cgcagccccg gcctgaatca
    32401 tattgatccg ggctgaacgc aaaaaatctg aactgttcgc taacccggtc cgaatctcaa
    32461 tctccgcagt aacatcacgc tgatcgcgat cggcctgcca gaggaaatag ccagccaggg
    32521 ctgaacttaa tgcgaataga actaagatac cgcctagaat gcaggcaaat aacggaacca
    32581 acctgatgtg atgcaaaaaa cctaatcgct gtgagggagt tgtattcatt accgttgtct
    32641 ctcgtccagg tttactcctg atagaaagtc atcggcattt gacggtaatt acttacgact
    32701 aaaagcctct ttgcgtcaca tttttacaac aattattgca gagaagatta atgccattgg
    32761 aatgaagaga gtaacgaatg gttaaactgg ctaacccgaa tgacgaaaaa ttctggaaaa
    32821 cagcacgcaa tttcgtcagg tgattatttt cgtccctaag gaaaccacat aggatgctca
    32881 cgggtgcctg agactttctg ttttcaggct aaccgggaat cagaaagacg aaggccccga
    32941 ccgatatttc tatcaaccga ggccaacgaa tgaaccaagc aattcagatg ttagcctctt
    33001 acccgccatc cggcaaggag aaaggctatg aagcgcaacc ctctggtggt gtgtctgctc
    33061 attatctgca ttacgattct gacattcaca ctcctgaccc gacaaacgct ctacgaactg
    33121 cggttccggg acagtgataa ggaggttgct gcgctcatgg cctgcacgtc caggtaaggg
    33181 caagcgcggg gattttcccc gcgcatctga ctagcagatt cattttctca tggcacccct
    33241 tttacacttc agttagtgca tgtttttttc gatgcactaa tagaaccatc attacagaca
    33301 aatttgccgc ctgccgtaca gtgtgagatc ccccctttcg aaccagaaca gggtttgcgc
    33361 ccggcatcgg cataaaagga aacaagcgct cccataaaca aaataagtgc cagttttttc
    33421 atataacctc catggtttat cgaatcttat gttcgatttg aacgttatac gctggtttat
    33481 tttgataccg ttgatgagat caaagatcgg gtgaattgat taaaaataag ccagatggac
    33541 gtatgggcaa ggattatggt tttatttgtc atacaaataa atataatagg cgcttccgat
    33601 gtagacccgt attcttcgcc tgtaccacgg gtcggtttta gtacaggcgt tttctttaaa
    33661 tatccttcag gaacgtttac gcggcatcat gcgtagaagt gtgttgtcct tccagaaata
    33721 gtggtgtgcc agtgcagccg cagcgtgcaa accgatgaca aaatatccca gattcgccag
    33781 cgtttcatgc cacgacttta agctatctac ccgttcgaaa ttggcctctg aagcgtaagg
    33841 catcgtcaaa ccaaacgcaa accacgggtt gcctcggtta tacatcatca ccaaaccaat
    33901 cactggcagc gcaataaaca ggagataaat caccaaatgt cccaaatgcg ccagtcccgt
    33961 catcatcggt tttggcttag gtataatcgg cggggttggg tatttcagcc ttaacaaaag
    34021 acgaacgacc atcagcacga gaattgatat gccgcaggaa acatgaatca tgttgatgag
    34081 tggccgatcg ctacgtggga agaaaccccg aaactccatt gcgcaatacg ctgcgataac
    34141 cagcaaaaag accagccagt gaatgctgat ttgtaacctt gagtatttat tttccataac
    34201 atttcctgct ttaacataat tttccgttaa cataacgggc ttttctcaaa atttcattaa
    34261 atattgttca ccctttttca gtacgttacg tttcactgta agaatggaga ttgcccatcc
    34321 cgccatcctg gtctaagcct ggaaaggatc aattttcatc cgaacgttcc tgacaggaga
    34381 aaaccatgag taaagttggt attaacggtt ttggtcgtat cggtcgactg gtgttgcgtc
    34441 gattacttga agtcaaaagc aacatagacg ttgtcgctat taatgatctt acttccccaa
    34501 aaattctcgc ctatctgctg aaacatgatt caaactatgg tccgttcccc tggagcgttg
    34561 attttacgga agattcactt atcgttgatg ggaaaagtat cgcggtttac gccgaaaaag
    34621 aggctaaaaa tattccgtgg aaagcgaaag gtgcagaaat cattgtcgaa tgtactggct
    34681 tttatacctc cgccgagaaa tcgcaggcgc atcttgatgc tggcgcgaag aaggtgttga
    34741 tttccgcccc tgcaggtgaa atgaaaacta tcgtttataa agtcaatgac gacactctgg
    34801 atggcaacga caccattgtt tccgtggcat catgcactac taactgtctt gcgccgatgg
    34861 ccaaagcctt gcatgacagt ttcgggatag aagtcggcac gatgacgacc attcatgcct
    34921 atactggcac ccagtcactg gtggatggcc cgcgtggtaa agatttacgt gcttcacgcg
    34981 ccgcggcaga aaatatcatt ccccacacta cgggcgcggc aaaagccatt ggtctggtga
    35041 tcccggaact gagcggcaaa ctgaaaggtc atgcgcaacg cgtgccggtg aaaacaggtt
    35101 cggtcactga actggtatcg attctcggaa aaaaagtgac tgccgaagag gtgaataacg
    35161 cacttaaaca agcaaccacc aataacgagt catttggtta taccgatgaa gaaatagtct
    35221 cttccgatat cattggcagc catttcggtt cggtgtttga tgccacgcaa acggaaatta
    35281 ccgccgtggg cgatttacaa ctggtgaaaa cggtcgcctg gtacgacaac gaatatggct
    35341 tcgtcacgca gcttattcgc accctcgaaa aattcgctaa actctgacgc gcacaggcgg
    35401 aggaaaaaac ctccgcctct ttcactcatt aagactgtaa ataaaccacc tgggtctgca
    35461 gatattcatg caggccatgt ttaccatctg cgccgccaat accggattta cgccatccgg
    35521 cgtggaagcc ttgcatagct tcgaagtttt cacggttgat gtaagtttca ccaaacttca
    35581 gccctttaat ggctttcatc gcgacgttca gattttgggt atagattgat gaggtcaggc
    35641 cgtaatcact gtcattagcc attgagatag catcttccag cgtgtcaaat gcgactaccg
    35701 gcagaaccgg gccaaaggtt tcctcatgca taatcgacat ttcctggcga acatccagca
    35761 gcaatgtcgg cggataataa tatcctttcc cctctaccgc tttgccaccc aacgccactc
    35821 tcgccccttc ttctactgcg cgcgccactt tttgctcgac cctttccagc gccgcggcgt
    35881 taatcaacgg ccccatcgca atgtcgttgc gttcagcggg gttaccaaat tgaaccgcct
    35941 gcatcgcttc acccagccga ttgacgaact gatcataaat gcctttctgt acataaacac
    36001 gttctgcaca gttacacact tgcccactat taatgacgcg tgaatcaacg atggctttga
    36061 ctgccagttc aagatcggca tcgtccatta cgatagctgg tgctttaccc cccagttcca
    36121 ggcacacttt ggtgatgttt ttcgccgcag tcgccatgat cttctcacct gcagagacgc
    36181 tgcctgtcat actgaccatt gcgacctttg ggttacccgc cagttcttgc ccaacggttt
    36241 caccacgccc cagaacaagg ttaaacacgc cgcgcggaag gcctatttca tcgacgattt
    36301 tggcgaatgc aatcgcattg tttggcgtaa attcactggg tttaatgaca atggtattac
    36361 cagtcaaaag agcgggagcc attttgcggg caatgaggaa gaacgggaag ttccacggca
    36421 gaatgccggt agtcacacca agcgcacgtt taaacagaag aatattttct cctggacgat
    36481 cgctttgaat aatctcgccc tcgtaacgcc gtgcccactc cgccatgtaa tcgatatagt
    36541 cggcagtaaa agccacttcg acttcagcca gctgctggat cttgccccct tcttcaacaa
    36601 tcagcgcact gatttcactg gcgcgttcgc ggatcccggc ggagattttg cgcaaccaac
    36661 tggcgcgttc aattgcaggc aacgcttccc attctggttg tgcacgttct gctgcatcga
    36721 ttgccttacg ggcatcctcg gcctgaccat cgggaatgcg ggaaatgaca gcctctgtag
    36781 cagggtttac cacatcaatc catgcgtctc cacgccaggt aacaaactgt ccatcaatat
    36841 acataggatg ttgaacgggt actgacatgg gcgactcctg tgatttatat gtttgttttt
    36901 ggcaaataca ttgttaatta acatcaatca tcatagagac ggcatcatga aaggacaatt
    36961 ctgtgaaggg cttcataaaa acaagtgtaa aaggaataag aggcagaaat aaaaggcagg
    37021 agttatgccc gtttgcaaac aagtggttca caaacgggca atttttaacg taacgagcga
    37081 ctttccatcg cctcgatgag caccgcatcg tgtttcagcg tttgccatgc atggatgact
    37141 tctgccggaa aatcaacgtg aacgataaaa tctcgcccgc ggggaccgta gccatcgcta
    37201 tcatcgcgta aacgcggcag ctcgccagtg agtagtgaga gataacgctc aaccggccat
    37261 aatccttgta actgattaat aaagattaca ctgtctgcgt tatttcctaa ctgaggaacg
    37321 aatccgggat aacttaacca gcgtggtaca tcgggattgt ccccggtcat acggcggaac
    37381 gtcgccatcg tgcgccgctg catggtgggg tcctgtacaa cgatagctgt gtgaactcgt
    37441 tctacggcct gattcaatag cgcgatgcta aagcgtgcgt tttcaccgca gtttgttgac
    37501 tggtcttcaa tccagatttt ttcatgcgga atatgccaga actgatgagc gatatccgcc
    37561 aggatggtcg cttctgctct gccagtggtg cggatagtgt tgtagtgcgg atgctgtgcg
    37621 atggcgctat acaaaaaagt ggtcgagtga ccgataccac cactaatcag taaaggaatt
    37681 tgctgatcgc gagcaatctt acatgccgca tcgatagtcg gcataaccgc atttcctgca
    37741 agtatgacgc aatcagcctg atacggcacc tcaccggaga aatcatcctg cgccagccac
    37801 tgtccgataa catttatggc atctatagtt gccgtcgaaa gcgtcggaaa cggggtaatg
    37861 ttcatggttc ctccttatcg ttcccttaag aatactgcgt gagataatag tgagaagcgg
    37921 agcactctga aaggaagata atgctttttc tatctcgcca tcgcgaacca gacactggtt
    37981 ttagtcatac actatttgcg gagaaactgt tgataaaaca tcaataacat catttgacgt
    38041 ggcaataaca taatgttcga tcattttatt tttatgaatt tcacaacttt gcatagtaaa
    38101 ccatgtaaga taagtggaat tggctactct atagatcccc gtttggttaa ttccctgctt
    38161 tcctgacata cctaacaaat caccttcgtc agtaacacga aatgagtgta tccagtcaaa
    38221 tgatatctta acttctgact tgatcgccgg gcatatcagg gccagttcaa gcctggcatt
    38281 cttatagctt aaacctgaca gatcggcgtg tttaaaagac aacgaagtcg gcgtgatatt
    38341 tataaaacgt t
//
LOCUS       DBFIFI010000022        37752 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.022, whole genome shotgun sequence.
ACCESSION   DBFIFI010000022 DBFIFI010000000
VERSION     DBFIFI010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37752
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.022"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..779
                     /gene="ytfI"
                     /locus_tag="N7B39_001128"
     CDS             <1..779
                     /gene="ytfI"
                     /locus_tag="N7B39_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YtfI"
                     /protein_id="HGU2421145.1"
                     /translation="EFFRCATQIDKSSYQVLSSKNETVNAMDKFLISFSLKDNGAEYT
                     MTLRGSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYAWDILQKKQSDIPKRIKG
                     YIHQAIGDVSLGYALLDDIVSKLKRGKFERQGPGGGIKQCDGWYIYEKIIDDNFAIVI
                     ESLGFALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANL
                     AADSDITKEKICEKYKSTIEAFKKEQLALPVLVRRKNSET"
     gene            complement(842..1396)
                     /gene="ytfJ"
                     /locus_tag="N7B39_001129"
     CDS             complement(842..1396)
                     /gene="ytfJ"
                     /locus_tag="N7B39_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HGU2421146.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWLLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLQKLLK"
     gene            1721..1927
                     /gene="ytfK"
                     /locus_tag="N7B39_001130"
     CDS             1721..1927
                     /gene="ytfK"
                     /locus_tag="N7B39_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HGU2421147.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            complement(2006..3349)
                     /gene="paeA"
                     /locus_tag="N7B39_001131"
     CDS             complement(2006..3349)
                     /gene="paeA"
                     /locus_tag="N7B39_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HGU2421148.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANI
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(3672..4310)
                     /gene="msrA"
                     /locus_tag="N7B39_001132"
     CDS             complement(3672..4310)
                     /gene="msrA"
                     /locus_tag="N7B39_001132"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HGU2421149.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLHGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            4516..6249
                     /gene="tamA"
                     /locus_tag="N7B39_001133"
     CDS             4516..6249
                     /gene="tamA"
                     /locus_tag="N7B39_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HGU2421150.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            6246..10025
                     /gene="tamB"
                     /locus_tag="N7B39_001134"
     CDS             6246..10025
                     /gene="tamB"
                     /locus_tag="N7B39_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HGU2421151.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGILNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQIYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGLEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEIPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELTVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTLVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTEEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            10028..10369
                     /gene="ytfP"
                     /locus_tag="N7B39_001135"
     CDS             10028..10369
                     /gene="ytfP"
                     /locus_tag="N7B39_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HGU2421152.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            10581..10832
                     /gene="chpS"
                     /locus_tag="N7B39_001136"
     CDS             10581..10832
                     /gene="chpS"
                     /locus_tag="N7B39_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin ChpS"
                     /protein_id="HGU2421153.1"
                     /translation="MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEVQVSNNQLILT
                     PISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGDEIW"
     gene            10826..11176
                     /gene="chpB"
                     /locus_tag="N7B39_001137"
     CDS             10826..11176
                     /gene="chpB"
                     /locus_tag="N7B39_001137"
                     /EC_number="4.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease toxin ChpB"
                     /protein_id="HGU2421154.1"
                     /translation="MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLG
                     MTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVRMMDLRARLAKRIGLAADE
                     VVEEALLRLQAVVE"
     gene            complement(11256..11786)
                     /gene="ppa"
                     /locus_tag="N7B39_001138"
     CDS             complement(11256..11786)
                     /gene="ppa"
                     /locus_tag="N7B39_001138"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HGU2421155.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            12096..13052
                     /gene="ytfQ"
                     /locus_tag="N7B39_001139"
     CDS             12096..13052
                     /gene="ytfQ"
                     /locus_tag="N7B39_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HGU2421156.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMMDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            13192..14694
                     /gene="ytfR"
                     /locus_tag="N7B39_001140"
                     /pseudo
     CDS             13192..14694
                     /gene="ytfR"
                     /locus_tag="N7B39_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
     gene            14708..15730
                     /gene="ytfT"
                     /locus_tag="N7B39_001141"
     CDS             14708..15730
                     /gene="ytfT"
                     /locus_tag="N7B39_001141"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HGU2421157.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIIT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLILFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            15717..16712
                     /gene="yjfF"
                     /locus_tag="N7B39_001142"
     CDS             15717..16712
                     /gene="yjfF"
                     /locus_tag="N7B39_001142"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HGU2421158.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPVYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQQ"
     gene            complement(16745..17743)
                     /gene="fbp"
                     /locus_tag="N7B39_001143"
     CDS             complement(16745..17743)
                     /gene="fbp"
                     /locus_tag="N7B39_001143"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HGU2421159.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYSTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFICEFPDA"
     gene            17919..19292
                     /gene="mpl"
                     /locus_tag="N7B39_001144"
     CDS             17919..19292
                     /gene="mpl"
                     /locus_tag="N7B39_001144"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HGU2421160.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(19448..19999)
                     /gene="yjgA"
                     /locus_tag="N7B39_001145"
     CDS             complement(19448..19999)
                     /gene="yjgA"
                     /locus_tag="N7B39_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HGU2421161.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            20093..21445
                     /gene="pmbA"
                     /locus_tag="N7B39_001146"
     CDS             20093..21445
                     /gene="pmbA"
                     /locus_tag="N7B39_001146"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HGU2421162.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            21629..22015
                     /gene="cybC"
                     /locus_tag="N7B39_001147"
     CDS             21629..22015
                     /gene="cybC"
                     /locus_tag="N7B39_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HGU2421163.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNSYHKKYR"
     gene            complement(22060..22524)
                     /gene="nrdG"
                     /locus_tag="N7B39_001148"
     CDS             complement(22060..22524)
                     /gene="nrdG"
                     /locus_tag="N7B39_001148"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HGU2421164.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(22714..24852)
                     /gene="nrdD"
                     /locus_tag="N7B39_001149"
     CDS             complement(22714..24852)
                     /gene="nrdD"
                     /locus_tag="N7B39_001149"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HGU2421165.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDISEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(25246..26901)
                     /gene="treC"
                     /locus_tag="N7B39_001150"
     CDS             complement(25246..26901)
                     /gene="treC"
                     /locus_tag="N7B39_001150"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HGU2421166.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPDDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVALNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DESSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQLGQMRGNWQLVMHNYEEASPQPCAMTLRPFEAVWWLQK"
     gene            complement(26951..28372)
                     /gene="treB"
                     /locus_tag="N7B39_001151"
     CDS             complement(26951..28372)
                     /gene="treB"
                     /locus_tag="N7B39_001151"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HGU2421167.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(28491..29438)
                     /gene="treR"
                     /locus_tag="N7B39_001152"
     CDS             complement(28491..29438)
                     /gene="treR"
                     /locus_tag="N7B39_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HGU2421168.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPANLS"
     gene            29623..29676
                     /gene="mgtL"
                     /locus_tag="N7B39_001153"
     CDS             29623..29676
                     /gene="mgtL"
                     /locus_tag="N7B39_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HGU2421169.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            29817..32513
                     /gene="mgtA"
                     /locus_tag="N7B39_001154"
     CDS             29817..32513
                     /gene="mgtA"
                     /locus_tag="N7B39_001154"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HGU2421170.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTVVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(32719..33105)
                     /gene="ridA"
                     /locus_tag="N7B39_001155"
     CDS             complement(32719..33105)
                     /gene="ridA"
                     /locus_tag="N7B39_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HGU2421171.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(33178..33639)
                     /gene="pyrI"
                     /locus_tag="N7B39_001156"
     CDS             complement(33178..33639)
                     /gene="pyrI"
                     /locus_tag="N7B39_001156"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HGU2421172.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(33652..34587)
                     /gene="pyrB"
                     /locus_tag="N7B39_001157"
     CDS             complement(33652..34587)
                     /gene="pyrB"
                     /locus_tag="N7B39_001157"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HGU2421173.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(34591..34725)
                     /gene="pyrL"
                     /locus_tag="N7B39_001158"
     CDS             complement(34591..34725)
                     /gene="pyrL"
                     /locus_tag="N7B39_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HGU2421174.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(35006..35401)
                     /gene="yjgH"
                     /locus_tag="N7B39_001159"
     CDS             complement(35006..35401)
                     /gene="yjgH"
                     /locus_tag="N7B39_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HGU2421175.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPTWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(35532..36245)
                     /gene="bdcA"
                     /locus_tag="N7B39_001160"
     CDS             complement(35532..36245)
                     /gene="bdcA"
                     /locus_tag="N7B39_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU2421176.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            36316..36909
                     /gene="bdcR"
                     /locus_tag="N7B39_001161"
     CDS             36316..36909
                     /gene="bdcR"
                     /locus_tag="N7B39_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGU2421177.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLVDILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETAIYDYIARLHPQSAQCVTDFM
                     STVMSGLSAKAREGHSLEQLCATAALAGEAIKTILKE"
     gene            37054..37506
                     /gene="tabA"
                     /locus_tag="N7B39_001162"
     CDS             37054..37506
                     /gene="tabA"
                     /locus_tag="N7B39_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HGU2421178.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            37629..>37752
                     /locus_tag="N7B39_001163"
     CDS             37629..>37752
                     /locus_tag="N7B39_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2421179.1"
                     /translation="MSKISDLNYSQHITLADNFKQKSEVLNTWRVGMNNFARNAG"
BASE COUNT         9119 a         9362 c        10133 g         9138 t
ORIGIN      
        1 gggagttctt tcgttgcgca actcagattg ataaaagtag ttatcaggtg ctttcatcaa
       61 aaaatgaaac tgttaacgct atggataagt ttcttatcag tttcagcctt aaagataacg
      121 gtgccgaata tacaatgaca ttgcgaggca gtggatttga atacgaagaa atccccataa
      181 caataaatga gtataactca ttcatggatt tcaagaaccg tgaatttccg ttagaacaaa
      241 acagacgatt gtatgcctgg gatattctgc agaaaaaaca gtctgacata ccgaaaagga
      301 ttaaaggtta tattcaccag gcgattggtg acgtgtcttt aggttatgct ttattggatg
      361 atatagtaag caaattaaaa cggggtaaat ttgaacgaca agggccagga ggtggaatta
      421 aacagtgcga tggatggtat atttacgaaa aaataattga tgataatttt gccatagtca
      481 tagaatcttt gggctttgcg cttaaaatct atggagggga tgaacgtttt cgtaatggtt
      541 cctctgttgt cttggaggat gaagattact ctcttattta taattttctg gttaatgcgg
      601 gttgccaaca agtcgaatta gccgaacaag tggatgcgat agtaagtgcg aatctggccg
      661 cagatagtga tattaccaaa gaaaagattt gcgagaaata taaatcgacc attgaggcgt
      721 ttaaaaaaga gcaactagcg ttaccagtat tagttcggcg taagaactca gagacttaat
      781 tcccctatcc catagataac gataggggaa aattattttt gcagaattta tgttattgcc
      841 attatttgag caacttttgc agcaggtcca tcacttgctg cacctcttcc tgagtgagcg
      901 ccccgtcttt ggcccattgc acgcgaccgt ctttatccag caccactaca gcggaacttt
      961 cttcatccag cagccaggca ccgcgcgcga cgccattgct atcgacaata aactgcgacc
     1021 agggataaag ctttttatta ctctccagac tgctgcgcac aaacatgccg gaacccggaa
     1081 tcgcgtcgtc ggtgttaaca atggtggtgg tctggtaacg atcgtgcggt aacttcgctg
     1141 atttaatcgc ttcaatcagc gtcgcgtttt tctcttttgc agaggtgcga ccagcaatat
     1201 gttgcagtac tcgcactttt cccactaact gcgcgctgtt ccaggttttg tagctaaact
     1261 gatctttatc aagcaccaac tcgccccgat cggtaatgcc aatcggcggc actcgctgac
     1321 cggtttcgaa ctgatgtgcg gaagccatca tcggcaacag caggcaggtg agtgccagaa
     1381 tcttgcgtag ggtcatggtg tttccttctt atgatatgca ggtgatccga ccacttgggt
     1441 ctgagtttta atcatatgtg ctatttatcg aatttcccgc aagtgtgatg ccagtttgcg
     1501 gtcaagcgca caaatcatat gaaaaatgaa tgcttatact gaagaccgcg cttcggtaaa
     1561 aagataattc tgaataattg taacctttag gtaaaaaaag ttatacgcgg tggaaacatt
     1621 gcccggatag tctatagtca ctaagcatta aaatttgcgc ctcataatag ttgggccgat
     1681 tgtggcaccg cacaggcgta atactcagca ggagataaca atgaaaattt tccaacggta
     1741 caacccactt caggtggcga agtacgtaaa gatcctgttc cgtggacgat tatacatcaa
     1801 ggacgttggc gcttttgaat tcgataaggg taagattctt atcccaaaag tgaaggataa
     1861 gctgcatttg tcagtgatgt ccgaagttaa ccgtcaggtt atgcgtctgc aaacagagat
     1921 ggcttaacca aagtgctatg cagtaaaaag tgctatgcag taataagacg gctcctgatt
     1981 caggagccgt tgatgtttat gggggttacg cgacgctttc ttctttatct ttagcgtcag
     2041 gcagttttgg cgaaagggct gtggccttgc tgtcgatccg ggtcaccagc agctggtcga
     2101 tgcggtagtt atcgatatcc accacttcaa atttgtagcc ggcgaatttc accgaatcgg
     2161 tgcgtttcgg gatcttacgc agcataaaca tcataaagcc gccgatggtt tcgtagttgc
     2221 ccgactgcgg gaactcgtca atatccagca cgcgcatgac gtcgtcaatc ggagtgccgc
     2281 cgtcaatcag ccatgagttc tcatcacggg cgacaatctg ctcttccagt ccctgaccga
     2341 ccagatcgcc catcagcgtg gtcatcacgt cgttgagggt gatgatcccc actaccagcg
     2401 cgtactcgtt catgatcacc gcgaagtctt ctcctgcggt tttaaaactt tccaacgcct
     2461 ctgaaagggt taacgtatcc ggcacaatca gcgtgttgcg aatttgtacg ccgctgttca
     2521 gtgccaggct ttggttagcc agcacgcggt tcagcaggtc tttagaatcg acatagccga
     2581 tgatgtggtc aatatcttca ttacagacga ggaacttaga gtgcggatgt tccgccacct
     2641 tatttttcag actttgctca tcttcgtgga gatcaaacca aatcacgttt tcacgcggtg
     2701 tcattgaaga cggaacggta cgggattcca gctcaaagac gttttcaatc agctcgtgtt
     2761 cctgtttacg taacacgccc gccagcgcac cggcttccac taccgcgtag atgtcatcag
     2821 aagtgatgtc atctttacgt accattggca gtttgaaaat acggaagatt atgttcgcca
     2881 ggccgttgaa gaaccacacc agcggggtgc aaacgtacag gcagaagcgc atcgggttga
     2941 tgatacgcaa agccacagct tctggcgcaa tcataccgat gcgtttcggg gttaaatccg
     3001 caaacaggat aaacatgcca gtcactaacg agaaagagag aataaagctc agttgctcag
     3061 agagctctgc cgacatatag cgggagaaca ggctgtgaaa agctggagaa aatgccgcat
     3121 caccgacgat accgccgaga atcgccactg cgttcagacc gatttggacc acggtaaaga
     3181 acatgccggg attttcctgc atattcagaa cgcgttgggc atttatattg ccttcatcag
     3241 ccagcagttt aagtttgatt ttgcgtgagg cggcaagtga gatctcagac atcgagaaga
     3301 acgcacttac agcgatcaag cagagtatga ctaaaatact gtttaacata tcttatccga
     3361 cctttcaggc cagatcctcg gaaggggaag ttgattaatt tgtgtgaaat acacattgaa
     3421 agccgattcg aagaaagtga accggcattt tcagtggcta gtatagcgta aggtactgta
     3481 aagccgccag agggttaaaa ttcagacagc tgaaaaatgc aaaactgcct gatgcgctat
     3541 gccgatcagg cctacgcagt ccttgcaata tattgaattt ggacgatttt gtaggcctgg
     3601 caaggcgttt acgccgcatc cggcataaac aaaacgcact ttatcaacaa tctacatttg
     3661 tacccgtaac gctatgcttc cggtggcaga cagacgccaa ttccgccaat tccacagtaa
     3721 ccgtacgggt ttttatgcag atattgctgg tggtcatctt cggcataata aaacggtgtg
     3781 gcgttagcga tttccgtggt gatgtgacga tcgtcatcgg cggcaagcat cgccgcctga
     3841 aaacgttcca gactggcgcg agctgcggca tcctgttctg gggtcagcgg ataaatcgct
     3901 gaacgatact gcgtgccgtg gtcattgccc tgacgcattc cctgggcggg atcgtgattc
     3961 tcccaaaata cctgtagcaa ctgctcatag ctgatgacgg aaggatcgta aacaatgcgt
     4021 accgcttcgg catgaccagt atcaccggag cacacttccc gataagtcgg atttggcgta
     4081 tagccgccgg tataacctgc ggcggtgctg taaacgccgt gtaactgcca gaacagacgc
     4141 tccacacccc agaaacaacc catcgcaaaa atggcaatct ccattccgtc aggtacattg
     4201 gtcattgagt gaccgttaac cgcatgcagc gtggctacgg gcatcggggt gttacgtcca
     4261 ggcagggcat cggcggggga aaccagatgc tttttatcaa ataaactcat ggtgtcgctc
     4321 tcccgaaaat cggtcattgg ggttaaggtt gtaacaagag gcgtatttgc acacaataac
     4381 caccgtgaat aggtctaaag taaaacataa gaaatatttg gggtttagtc tgctttttaa
     4441 tccatattac tggatttttg ttaagccgtt taacggcgtt ccaggggcag gaaaaaagga
     4501 tattcaggag aaaatgtgcg ctatatccga cagttatgct gtgtaagctt actctgctta
     4561 agcggatctg ccgtcgccgc gaacgtccgt ctacaggtcg aggggttatc gggacagctg
     4621 gaaaagaacg ttcgtgcgca gctttctacg attgaaagtg atgaagtgac gccagaccgt
     4681 cgctttcgcg cacgcgtcga tgatgctatc cgcgaaggtc tgaaagcgct gggttattac
     4741 cagccgacca ttgaatttga tctccgtcca ccgccaaaga aagggcggca ggtattgatc
     4801 gccaaagtca cgccaggcgt gccggtgtta attggcggca ccgatgtggt attgcgcggg
     4861 ggcgcgcgga ccgataaaga ctatttgaaa ttgctcgata ctcgcccggc tattggcacg
     4921 gtactgaacc agggcgatta tgaaaatttc aaaaagtcct taaccagcat tgcgttgcgt
     4981 aaaggttatt tcgatagcga atttaccaaa gcgcagctgg gcattgcgct cggcctgcat
     5041 aaagccttct gggatattga ttataacagt ggcgaacgtt accgctttgg gcatgtgacc
     5101 tttgaaggat cacaaatccg cgatgaatac ctgcaaaatc tggtgccgtt taaagagggc
     5161 gatgagtacg aatcgaaaga tctggcagaa ctgaaccgcc gactttctgc taccggctgg
     5221 tttaactcgg tggtggtggc tccacaattt gataaagcgc gcgaaacgaa agtattacca
     5281 ttgacgggcg tggtttcgcc gcgaacagaa aacactatcg aaaccggggt cggttactct
     5341 acggacgtgg gaccgcgcgt gaaagcgacg tggaaaaaac cgtggatgaa ctcatacggt
     5401 cacagtctga ccaccagtac cagtatttcc gcgccggaac agaccctcga cttcagctat
     5461 aaaatgccgc tgctgaagaa tccactggaa caatattatt tggtgcaggg cggttttaag
     5521 cgcactgacc tgaacgatac cgagtctgac tccactacgc tggtggcttc tcgctactgg
     5581 gatctctcca gcggctggca gcgtgccatt aacctgcgct ggagtctcga ccactttact
     5641 cagggtgaaa ttaccaacac cacgatgctg ttttatcctg gggtgatgat tagccgcacg
     5701 cgttctcgtg gtggcctgat gccaacctgg ggcgactcgc aacgctactc tatcgactac
     5761 tccaacacgg cctggggttc agatgtcgat ttctccgttt tccaggcgca gaacgtctgg
     5821 atccgcacac tgtacgatcg ccatcgtttt gtgacacgcg gcacgctggg ctggattgaa
     5881 accggtgatt tcgacaaagt accgccggat ctgcgtttct tcgccggggg cgatcgcagt
     5941 attcgcggct acaaatacaa atctatcgcc ccgaaatacg ctaacggtga cctgaaaggg
     6001 gcctcgaagt tgataaccgg atcgctggaa taccagtaca acgtgaccgg aaaatggtgg
     6061 ggcgcggtgt ttgtcgatag tggcgaagcg gtaagcgata ttcgccgcag cgactttaaa
     6121 accggtaccg gggtcggcgt acgctgggaa tcgccggtcg ggccaatcaa actcgatttt
     6181 gccgtaccgg tcgcggataa agacgaacac gggttacagt tttacatcgg tctggggcca
     6241 gaattatgag tttatggaaa aaaatcagcc tcggcgtggt tatcgttatc ttactgttgc
     6301 tgggatcggt ggcgtttctg gtgggcacca ccagcggcct gcatctggta tttaaagcgg
     6361 cggatcgctg ggtgccagga ctggatattg gcaaggtcac cggcggctgg cgcgatctca
     6421 ccttgtctga cgttcgttat gagcagccag gcgtggcggt aaaagcgggt aatctgcatc
     6481 tggcggtcgg tcttgagtgc ctgtggaaca gcagtgtttg tattaatgac ttggcgctga
     6541 aagacattca ggtcaacatc gacagtaaaa aaatgcctcc ttctgaacag gttgaagaag
     6601 aggaagatag cggtccgctg gatctctcca cgccgtatcc catcaccctg acacgggtgg
     6661 cactggacaa cgtcaacatc aagattgatg acaccacggt atcggtgatg gacttcacct
     6721 ccggcctgaa ctggcaggag aaaaccctga ccctgaaacc gacgtcgctg aaaggcctgc
     6781 tgattgctct gccgaaagtg gcggaagtgg cgcaggaaga agtggtcgaa ccgaaaattg
     6841 aaaatccgca gccggaggaa aagcctctcg gcgaaacact caaagatctc ttttctcgcc
     6901 cggtattgcc ggaaatgacc gacgtgcatc tgccactcaa cctgaacatt gaagagttta
     6961 agggagagca actgcgcgtg acgggcgata cggacattac cgtgcgcacc atgctgctga
     7021 aagtgagcag cattgacggc aatactaaac tggacgccct ggatatcgat tccaatcaag
     7081 ggatcctcaa cgccagcggc acggcgcagc tgtcagacaa ctggccggtg gatatcaccc
     7141 tcaacagcac actgaacgtg gagccgttga aaggtgaaaa ggtgaagctg aaagtgggcg
     7201 gcgcgctgcg cgaacagctg gagattggcg ttaatctttc cggtccggtg gatatggatt
     7261 tacgcgccca gacgcgactg gcggaagccg gattgccgct caacgtggaa gtgaacagca
     7321 aacagattta ctggccgttc actggtgaga agcagtatca ggcggatgat ctgaaactga
     7381 aacttaccgg caaaatgacc gattacacgc tctctatgcg tacggcagtg aagggactgg
     7441 agatcccgcc agccaccatt acccttgatg ccaaaggtaa tgaacagcag gtcaatctcg
     7501 acaaactcac cgtcgcggcg ctggaaggga aaactgaact caaggcgttg cttgactggc
     7561 agcaggccat tagttggcgc ggtgagctaa cgcttaacgg cattaacacc gccaaagaga
     7621 tcccagagtg gccgtcgaaa ctcaatggct tgattaaaac ccgcggtagc ctgtacggcg
     7681 gcacctggca gatggaggtg ccagaactga agcttaccgg taacgtcaaa cagaacaaag
     7741 tgaacgttga cggcacgctg aaaggcaaca gttatatgca gtggatgatc ccagggcttc
     7801 atctggaact ggggccaaac agtgccgaag tgaaaggcga gctgggggta aaagatctca
     7861 atcttgatgc caccatcaac gcgccggggc tggataacgc gctgccgggg cttggcggta
     7921 cagcgaaagg gctggtgaaa gttcgcggca cggtggaagc gccacaacta ctggcggaca
     7981 tcaccgcgcg cggcctgcgc tggcaggagc taaccgtggc gcaggttcgc gtggaaggcg
     8041 atatcaaatc caccgatcag attgcgggta aactcgacgt gcgcgttgag caaatttcac
     8101 agccggatgt aaatatcaac ctcgtcaccc tgaatgccaa aggcagcgaa aaacagcacg
     8161 agttacagtt gcggattcag ggcgagccgg tttccgggca gcttaatctg gcaggaagtt
     8221 ttgatcgcaa agaagaacgc tggaagggaa ctcttagcaa tacccgcttc cagacgctgg
     8281 tcggcccgtg gtcgctgacc cgcgatattg cgctggatta ccgcaataag gagcaaaaaa
     8341 tcagcatcgg gccacactgc tggcttaacc cgaatgcgga actgtgcgtg ccgcaaacta
     8401 tcgatgcggg ggccgaaggg cgtgcggtgg tgaatctcaa ccgcttcgac cttgccatgc
     8461 tgaaaccgtt tatgccagaa accactcagg ccagcggtat cttcacgggt aaagcggacg
     8521 ttgcctggga caccaccgaa gaggggctgc cgcagggcag tatcaccctt tcggggcgta
     8581 acgtgcaggt aacgcaaacc gtcaacgatg cggcgctgcc cgtggcgttt cagacactga
     8641 atctgacggc ggaattgcgt aacaaccgtg ccgaattggg ctggaccatc cgcctgacca
     8701 ataacggcca gtttgatgga caggtgcagg tgaccgatcc gcaaggccgc cgtaatcttg
     8761 gtggcaacgt caatatccgt aacttcaacc ttgcgatgat aaaccccatc tttacccgtg
     8821 gggaaaaagc agcggggatg gtgagtgcca acttgcgtct gggtggtgat gtgcaaagcc
     8881 cgcagttgtt tggtcagctt caggttacgg gtgtggatat cgatggcaac tttatgccgt
     8941 ttgatatgca accgagccag cttgcggtca actttaacgg tatgcgctcg acgcttgccg
     9001 gtacagtacg aacccagcag ggtgaaatct acctgaacgg tgatgccgac tggagccaaa
     9061 ttgaaaactg gcgggcgcgg gtaacggcga aaggcagtaa agtgcggatc accgtgccgc
     9121 cgatggtacg aatggatgta tcgccagatg ttgtattcga ggctacacca aacctgttta
     9181 ccctcgatgg tcgcgtggat gtcccttggg cgcgcatcgt ggtgcacgat ctgccggaaa
     9241 gcgcagtagg cgtctccagc gatgtggtga tgcttaacga taacctgcaa ccggaagagc
     9301 cgaaaacggc gtcgattccg attaacagta acctgattgt ccacgttggc aacaatgtgc
     9361 gcattgacgc ctttggcctg aaagcgcggc tgacgggcga tcttaacgtc gttcaggaca
     9421 aacaagggct gggcctgaac gggcagatca acatccctga agggcgcttc catgcctatg
     9481 gtcaggatct gattgtgcgt aaaggtgagt tactgttctc tggcccgccg gatcaaccgt
     9541 atcttaatat tgaagctatt cgtaacccgg atgctacaga agacgacgta atcgccggag
     9601 ttcgcgtcac tggtctggcg gacgaaccga aagcggagat cttctctgac ccggcgatgt
     9661 cgcaacaagc tgccttgtct tatttgctac gtggacaagg gctggagagc gatcagagcg
     9721 acagtgcggc aatgacctcg atgctgattg gtctgggggt tgcgcaaagt ggccagattg
     9781 taggtaaaat cggcgagacg tttggcgtaa gcaatttagc gctcgacacc cagggagtag
     9841 gcgactcctc ccaggtagtg gtcagcggct atgtattgcc aggtctgcaa gtgaaatacg
     9901 gcgtgggtat atttgactct atagcaacac tcacgttacg ttatcgcctg atgcctaagc
     9961 tatatctgga agccgtgtct ggtgtagacc aggcactgga tttgctctat cagttcgagt
    10021 tttagcaatg cgaatatttg tctacggcag tttacgccac aaacaaggca acagtcactg
    10081 gatgaccaat gcccagttac tgggcgattt cagtatcgat aactaccagt tgtatagcct
    10141 gggccactat ccaggcgcag ttccggggaa cggaacggta cacggtgaag tttatcgtat
    10201 tgacaacgcc acgctggccg aacttgatgc cttgcgcacc aggggcggtg aatacgcgcg
    10261 ccagttgatt cagacgccgt acgggagtgc atggatgtac gtttatcaac gacccgtcga
    10321 tggattaaag ctaattgaaa gcggcgactg gttagacagg gataagtaac cctatgcata
    10381 cgccaccttc gggtggcgtt gttttttgcg agacgactcg cattctgttt tgtaattccc
    10441 tcaccttttg cttttctctc cgagccgctt tccatatcta ttaacgcata aaaaactctg
    10501 ctggcattca caaatgcgca ggggtaaaac gtttcctgta gcaccgtgag ttatactttg
    10561 tataacttaa ggaggtgcag atgcgtatta ccataaaaag atgggggaac agtgcaggta
    10621 tggtcattcc caatatcgta atgaaagaac ttaacttaca gccggggcag agcgtggaag
    10681 tgcaggtgag caacaaccaa ctgattctga cacccatctc caggcgctac tcgcttgatg
    10741 aactgctggc acagtgtgac atgaacgccg cggaacttag cgagcaggat gtctggggta
    10801 aatccacccc tgcgggtgac gaaatatggt aaagaaaagt gaatttgaac ggggagacat
    10861 tgtgctggtt ggctttgatc cagcaagcgg ccatgaacag caaggtgctg gtcgacctgc
    10921 gcttgtgctc tccgttcaag cctttaatca actgggaatg acgctggtgg cccccattac
    10981 gcagggtgga aattttgccc gttatgccgg atttagcgtt cctttacatt gcgaagaagg
    11041 cgatgtgcac ggcgtggtgc tggttaatca ggtgcggatg atggatctac gcgcccggct
    11101 ggcaaagcgt attggtctgg ctgcggatga ggtggtggaa gaggcgttat tacgcttgca
    11161 ggcggtggtg gaataaggtg tgtttattta tcgcgggcat aaaaaaaccc ttactaaccg
    11221 aagcccggcg ttcagggtta ttacgccaga agaacttatt tattctttgc gcgctcgaag
    11281 gaggcaacga tttcagcttt agcggcttct gcgttttccc aaccttcaac tttcacccac
    11341 ttgccttttt cgaggtcttt gtagtgctcg aagaagtgag cgatttgcgc tttcagcagt
    11401 tcaggcagat cgttaacgtc tttaatgtga tcgtattctt tgctcagctt gctgtgcgga
    11461 accgcaacca gtttcgcatc ttcaccggct tcgtcggtca ttttcagaac gccaaccgga
    11521 cggcaacgga tcacagaacc cggctgcagc gggtacggag ttgggaccag tacgtcaact
    11581 gggtcaccgt ccagagacag ggtgtggttg atgtaaccgt agttgcacgg atagaacatc
    11641 gcggtggaca tgaagcggtc aacgaacagt gcgccgctct ctttgtcgat ttcgtatttg
    11701 atcggatctg cgttagccgg gatctcaata acaacgtaga tgtcttccgg cagatcttta
    11761 cccgcaggga cgttgagtaa gctcatgtct gtttccttta aaaatatgtg gcaaacaagt
    11821 gccgagtatt atagccaact cgcgccgaat gtcttcgctt gttttcgtct tcattttcct
    11881 ttttcgccag ttttaagacg gtatgcagag caggaaaatc cataaccgta gccgcatttt
    11941 tcatagtgag atgaaagcga ttacaaactt gtgattaacg ttttatttac ttttttgaag
    12001 tgtgatgtaa cgcaatccgt tacataacga attgtctata gttttttcgc gaacatcttt
    12061 tcaccaataa taattactcc gacgaggaca accctatgtg gaaacgctta cttgtagtct
    12121 ctgcagtctc ggcagccatg tcgtctatgg cgttggccgc tccattaacc gtaggatttt
    12181 cgcaggtcgg atcggaatcc ggctggcgcg ccgcagaaac caatgtggcg aaaagtgagg
    12241 ccgaaaaacg cggaattacg ctgaaaattg ccgatggtca gcaaaagcag gaaaaccaga
    12301 ttaaagcggt acgttccttc gttgcacaag gggtggatgc gatctttatc gctccggtgg
    12361 tcgcgacagg ttgggaaccg gtattaaaag aggcgaaaga tgccgaaatc ccggtcttct
    12421 tgctcgaccg ttccatcgat gtgaaagaca aatctctcta tatgaccacc gttactgccg
    12481 acaacatcct cgaaggtaag ttgatcggtg actggctggt aaaagaagtg aatggcaaac
    12541 catgcaacgt ggtggagctg cagggcaccg ttggggccag cgtcgccatt gaccgtaaga
    12601 aaggctttgc cgaagccatt aagaatgcgc caaatatcaa aatcatccgc tcgcagtcag
    12661 gtgacttcac ccgcagtaaa ggcaaagaag tcatggagag ctttatcaaa gcggaaaaca
    12721 acggcaaaaa catctgcatg gtttacgccc ataacgacga catggtgatt ggtgcaattc
    12781 aggcaattaa agaagcgggc ctgaaaccgg gcaaagatat cctcacgggt tccattgacg
    12841 gtgtaccgga catctacaaa gcgatgatgg atggcgaagc gaacgccagt gttgaactga
    12901 cgccgaacat ggcgggaccc gctttcgatg cgctggagaa atacaaaaaa gacggcacca
    12961 tgcctgaaaa gctgacgtta accaaatcca ccctttacct gcctgatacc gcaaaagaag
    13021 agttagagaa gaagaaaaat atggggtatt gagggttgct atgcctgatg ccgattcgta
    13081 ggccggataa ggcgctcgcg ccgcatccgg cgatggtgca ctgaagcctg atgcgacgct
    13141 taccgcgtct tatcatgcct actgggagca cgctttacac cgggggaaac catgacgacc
    13201 gaccaacacc aggagatcct ccgcaccgaa ggattaagta aatttttccc cggcgtcaaa
    13261 gcgttagaca acgttgattt cagcctgcgc cgtggcgaaa tcatggcgct gctcggtgaa
    13321 aacggggcgg gaaaatcaac gctaatcaaa gcattaactg gtgtatacca cgctgatcgc
    13381 ggcaccatct ggctggaagg ccaggctatc tcaccgaaaa ataccgccca cgcgcaacaa
    13441 ctcggcattg gcaccgtcta tcaggaagtc aacctgctac ccaatatgtc ggtcgctgat
    13501 aatctattta taggccgcga acccaaacgc ttcggccttc tacgccgcaa agagatggaa
    13561 aagcgcgcca ccgaactgat ggcatcttac ggtttctccc tcgacgtgcg cgaaccgctc
    13621 aaccgctttt cagtcgcgat gcagcaaatc gtcgctattt gccgggctat cgatctctct
    13681 gccaaagtgc tgatcctcga tgaacccacc gccagtctcg acacccagga agtggagtta
    13741 ctgtttgacc tgatgcgtca gttgcgcgat cgcggcgtca gcctgatctt tgtcactcac
    13801 tttctcgatc aggtctatca ggtcagcgat cggatcaccg tcttacgcaa cggcagtttc
    13861 gtaggctgtc gggaaacgtg ctagctaccg cagatcgaac tggtaaaaat gatgctgggg
    13921 cgcgagctgg atacccacgc gctacagcgt gccgggcgaa cattgttgag cgacaaaccc
    13981 gttgccgcgt tcaaaaatta cggcaaaaaa ggaacgatcg caccgtttga tctcgaagta
    14041 cgccccggcg agatcgtcgg tctggctgga ttgctgggat caggacgtac cgaaaccgcc
    14101 gaagtgatct tcggtatcaa acctgctgac agcggcacgg cgttgatcaa aggcaaaccg
    14161 caaaacctgc gatcgccaca tcaggcttcg gtacttggca ttggcttctg cccggaagac
    14221 aggaaaaccg atggcatcat cgctgccgcc tcggtgcggg aaaatatcat cctcgctctc
    14281 taggcccagc gcggctggct acgtcccatt tcccgcaaag aacagcaaga gattgccgaa
    14341 cgctttatcc gccagcttgg cattcgcaca ccttcaactg aacaaccgat tgaatttctc
    14401 tccggcggca atcagcaaaa agtgttgctt tcacgttggc tactgacccg accgcaattt
    14461 ctgatcctcg atgagccaac ccgcggcatt gatgttggtg cccacgccga gatcatccgc
    14521 ctgattgaaa cgctatgcgc cgatggtctg gcgctgctgg tgatctcctc cgaactggaa
    14581 gaactggtgg gctatgccga ccgggtgatc atcatgcgcg atcgcaaaca ggtggcggag
    14641 atcccgctgg cagagctttc cgttccggcg atcatgaatg ccattgcggc gtaaggagaa
    14701 cagtgtgatg cctcaatctc tcccggacac tacgccgcca aaaaggcgct ttcgctggcc
    14761 aacgggaatg ccgcagctgg cggcactgtt gctggtgctg ctggtcgata gcctggtggc
    14821 cccgcatttc tggcaggtgg tgcttcagga tgggcgtttg ttcggtagcc ctatcgacat
    14881 tcttaatcgc gccgcccccg ttgcgctact ggcgattggt atgacgctgg tgatcgcaac
    14941 aggtgggatt gatctctccg taggggcggt gatggctatc gccggagcca caacggctgc
    15001 gatgacggtc gcgggattca gcctgccgat tgttttgtta agcgccctgg gcaccggcat
    15061 cctggcggga ttgtggaacg gcatactggt agcgatcctc aaaattcagc cgtttgtcgc
    15121 cactctgatc ctgatggtcg ccgggcgcgg cgtggcgcaa ctgatcacct ccggacagat
    15181 catcacgttt aactcgccgg atctctcatg gttcggcagt ggatcgctgt tgttcctgcc
    15241 aacgccggtc attatcgcgg tgctgacgct tatcctgttc tggctgttga cccgcaaaac
    15301 ggcgctgggg atgtttatcg aagccgttgg tatcaacatt cgggcggcaa aaaatgccgg
    15361 ggtaaacacg cggatcatcg tcatgcttac ctacgtgttg agcgggctgt gtgcggcgat
    15421 tgcgggcatt atcgtggcgg cggatattcg cggtgccgat gccaacaacg ccggattatg
    15481 gctggagctg gacgccattc tcgcggtggt gattggcggt ggttcgctga tgggcggacg
    15541 ttttaaccta ctgctttcgg tggtgggggc gctgattatt caggggatga acaccggaat
    15601 tttgctttcg ggctttccgc cagagatgaa ccaggtggtg aaagcggtgg tggtgctttg
    15661 cgtgctgatt gtccagtcgc aacgctttat cagtctgatt aaaggagtgc gtagccgtga
    15721 taaaacgtaa tttgccactg atgatcacca tcggcgtctt tgtgctgggt tatctctact
    15781 gcctgaccca gtttcccggt tttgcttcca caagagtgat ctgcaatatc ctgaccgata
    15841 atgcctttct tgggataatc gccgttggca tgacctttgt gatcctctcc ggtgggatcg
    15901 atctctccgt tggttcggtg atcgccttta ccggcgtgtt tctggcaaaa gtgattggcg
    15961 atttcggcct ctcgccgttg ctggcatttc cgctggtgct ggtaatgggc tgtgcctttg
    16021 gcgcatttat ggggcttttg atcgacgccc tgaagatccc ggcatttatc attactttgg
    16081 caggaatgtt ctttttgcgc ggcgtcagct acctcgtttc ggaagagtcg atcccgataa
    16141 accatcctgt ttatgacacg ctctcaagcc ttgcatggaa aattcctggc ggcggtcgct
    16201 taagtgcgat ggggctgctg atgctggcgg tggtggttat cggcatattc ctcgcgcatc
    16261 gtacccgctt tggtaatcag gtgtacgcca ttggcggcaa tgcgacgtcg gcaaacctga
    16321 tggggatttc cactcgcagc acaactattc gcatttatat gctctctacc ggactggcga
    16381 cactggcggg gattgtcttc tcgatttata cccaggccgg atacgcgctg gcgggcgtag
    16441 gtgtggaact ggacgctatc gcctcagtgg tgattggcgg tacgcttttg agcggtggcg
    16501 ttggaacggt attagggacg ctttttggcg tggcgattca gggactgatt cagacttaca
    16561 taaactttga tggtacgctg agctcatggt ggacgaaaat cgccatcggt attttgttgt
    16621 ttatttttat agcattacag cgtggattaa cggtgctgtg ggagaatcgt cagagttcgc
    16681 ctgtgacaag agtcaacatt gcgcagcaat aaaatcgcct ctccgtgtgg agaggcgcag
    16741 gagattacgc gtccgggaac tcacagataa agcgttcgac atcttcaacc atatggtcgt
    16801 tgccgacaaa gaatgaacgg cgctggtgca gggtttccgg gataatatcc agaatacgct
    16861 ctttgccatc gctcgcttta ccgcccgctt gttccgccag gaatgccatc gggttgcact
    16921 catacagcaa acgcagtttg ccgtccgggt ggctggcggt gcttgggtag agataaatac
    16981 cgcctttcag taggttacgg tggaaatccg caaccagtga gccgatataa cgtgaggtat
    17041 acgggcggtt ggtggattta tcttcttcct ggcagaattt aatgtacttc ttcaccccgt
    17101 tcggaaactt aatgtagttt ccttcgttga tggagtaggt tttgcctttc tccgggaagc
    17161 gcatccgttc ctggcacagg cagaaaacgc cgagcgaagg atcgtaagta aaagcgtgaa
    17221 caccgcatcc ggtactgtaa accagcatgg tagaggagcc gtataccacg taacctgccg
    17281 caacctgttt gttaccaggc tggaggaaat cttcttccgt taccggcgtg ccgacgggcg
    17341 taactcggcg gtagatggag aaaatggtac cgacagagac gttaacatcg atgttggacg
    17401 agccatccag tgggtccatc agcaccacgt attttgcatg ttcacagcct tcaaagacaa
    17461 caatctcatc ttcttcttca gaggcaatgc ctgcaacgat atcgcgtgct ttcagtgcgg
    17521 ctttcagttt ttcattagcg aacaagtcga gtttttgctg aacctcgccc tgcacgttct
    17581 cagcaccgct ggcacccagg atatcaacca gtcctgcttt gttgatatcg cgatggataa
    17641 tcttggcgcc cagttttatt gccgacagca aagcagtgag ctcaccggta gcatgagaaa
    17701 actcgtgctg cttttcgaca ataaattcac ctaacgtttt cataaaactt tccctgcaat
    17761 gtttatggag taaagcgacc gcaacaatct taacaaataa tctcaatgtt gcgctcaggt
    17821 gaatcgcgcc agcaaattac ggattatcct gaaatgcgtt tctcacttgc ccgacatatg
    17881 cgtaaaatga gcggcagatt aaaaaaggat agtgacgcat gcgcattcat attttaggaa
    17941 tttgtggcac gtttatgggc ggtctggcga tgctggcgcg ccagttaggc catgaagtaa
    18001 cgggttcgga cgccaatgtg tatccgccga tgagcacctt acttgagaag caaggcattg
    18061 agctgattca gggttacgat gccagccagc tcgacccgca gccggatctg gtgattattg
    18121 gcaacgccat gacccgtgga aatccgtgtg tggaagcggt actggaaaaa aacatccctt
    18181 atatgtcagg tccacagtgg ctgcacgatt ttgtgctgcg cgaccgctgg gtgctggccg
    18241 ttgccggtac acacggcaaa accaccaccg cgggaatggc gacttggatt ctggaacagt
    18301 gtggttacaa accgggcttt gtaatcggcg gtgtgccggg gaactttgag gtttcggcgc
    18361 gtctgggcga aagcgacttc tttgttatcg aagcggatga gtacgactgc gccttcttcg
    18421 acaaacgctc taaatttgtc cattactgcc cgcgtacgct gatcctcaac aaccttgagt
    18481 tcgatcacgc cgatatcttt gacgacctga aagcgatcca gaaacagttc caccatctgg
    18541 tgcgtatcgt tccggggcag ggtcgtatta tctggccgga aaatgacatc aacctgaaac
    18601 agaccatggc gatgggctgc tggagcgagc aggagctggt gggtgagcag ggtcactggc
    18661 aggcgaaaaa gctgaccacc gatgcttccg aatgggaagt tttgctggat ggcgaaaaag
    18721 tgggcgaagt gaaatggtcg ctggtaggcg aacataatat gcacaatggc ctgatggcga
    18781 ttgcagcggc tcgccatgtt ggtgtagcgc cggcagatgc cgctaacgcg ctgggttcgt
    18841 ttattaacgc tcgccgccgt ctggagttgc gtggtgaagc gaatggcgtc acggtatatg
    18901 acgattttgc ccatcatccg acggcgattc tggcaacgct ggcggcgctg cgtggcaaag
    18961 ttggtggtac ggcgcgcatt attgctgtgc tggagccgcg ctcgaatacc atgaaaatgg
    19021 ggatctgcaa agacgatctg gcaccttcat taggtcgtgc cgatgaagtc ttcctgctgc
    19081 aaccggcgca tattccgtgg caggtggcag aagtggcaga agcctgcgtt cagcctgcac
    19141 actggagtgg cgatgtggat acgctggcag atatggtggt gaaaaccgct cagcctggcg
    19201 accatattct ggtgatgagc aacggcggtt ttggtgggat ccatcagaaa ctactggatg
    19261 gtctggcgaa gaaggcggaa gctgcgcagt aattcggcct cagcctgaga tagtgtgcgt
    19321 aggccggata aggcgttcac gccgcatccg gcatttgagc atagtgcctg atgcgacgct
    19381 tgatgcgtct tatcaggcct acaggtgcac cgcatccggc aaaccatact cacatcaaca
    19441 acgaaaatta cccttcattc tccgccagtt cgcgcagata ctggaaaatc tggcgtgcgg
    19501 atttcggcgg cttattccct tctttctctt tcttcgcgtt gcggatcaga gtacgcagtt
    19561 gctgacgatc ggcatcgggc cacagattta atacctcagc gatggcgtca tcaccttgat
    19621 cgatcagacg atcgcgcagg ttttccagtt tatggaacag caccacctgc tggttgtgac
    19681 ggtttttcag cttatccagc gcctgacgga tcggttctac atcgcgctgg cgcagcatct
    19741 taccgatgag ctgcaactgg cggcggcgac cttccatctt aatacgctgg gcgagctcaa
    19801 tagccgcgcg cagatccgca tctaacggga tcttatccag cgcgtttttc cccagatcaa
    19861 caatttccgc gccaaggcgt tttagctcct cggcatcacg tttaatttca cttttactga
    19921 cccagataat ttcatcgtct tcgtcttcga tgtcatcacc gggaacgtcg tcgagccagt
    19981 cttcgggctg cttagtcatc tcaggctcct taaaaaaaga ggctaatgtt accagttaag
    20041 atgcgcactg aaaaacggtt ctctgttaga cttcagagaa actctctaca ttatggcact
    20101 tgcaatgaaa gtaatctctc aagttgaagc gcagcgcaag attctggaag aagcagtttc
    20161 cactgcgctg gagttggcct caggcaaatc ggacggtgcg gaagttgccg tcagcaagac
    20221 caccggcatt agcgtaagca cgcgttatgg tgaagtggag aatgttgaat tcaatagcga
    20281 tggcgcgctg gggatcactg tttatcacca gaaccgcaaa ggtagcgcat catccaccga
    20341 tttaagcccg caggccattg cccgtactgt acaggcggcg ctggatattg cccgttatac
    20401 ctcgccagat ccctgtgccg gcgtggcaga caaagagctg ctggcctttg acgcaccgga
    20461 tctcgacttg ttccaccctg cggaagtttc cccggatgaa gccattgaac tggcggcccg
    20521 cgcagaacag gcggcattgc aggcggacaa acgcatcacc aataccgaag gtggcagctt
    20581 taacagccac tacggtgtca aagtttttgg caacagccac ggcatgttgc agggttactg
    20641 ctcaacgcgt cattcgctct ccagctgtgt aattgccgaa gaaaatggcg atatggagcg
    20701 tgattacgcc tacaccattg gccgcgcgat gagcgatttg cagacgccag agtgggtcgg
    20761 ggccgattgc gctcgccgta ctttatcgcg tctgtcaccg cgtaaactct ccaccatgaa
    20821 agcgccggtc atttttgcca atgaagtggc aaccgggctt tttgggcatc tggtgggggc
    20881 gatagcgggt ggatcggttt accgtaaatc taccttcctg ctggattcgc tgggtaaaca
    20941 aattctgccg gactggctga ccattgaaga gcatccgcat ctgctgaaag ggctggcatc
    21001 gacgccattt gacagcgaag gtgtgcgcac cgagcgtcgc gatattatta aagatggcat
    21061 cctgactcag tggctgctga ccagctactc ggcgcggaaa ctggggctga aaagcaccgg
    21121 acatgcgggc ggtattcaca actggcggat tgccggacaa ggtctaagct tcgagcagat
    21181 gctcaaagag atgggcaccg ggctggtggt gacggaattg atgggccagg gcgtgagtgc
    21241 aattaccggt gattattccc gtggtgcggc gggcttctgg gtagaaaacg gcgaaattca
    21301 gtatccggtt agcgaaatca ccatcgcagg taatttaaaa gatatgtggc gcaatattgt
    21361 caccgtcggt aacgatattg aaacacgcag taatatacag tgtggttctg tgctgttgcc
    21421 ggagatgaaa atcgccggac agtaaaaaaa ctggcgcgac ctgccgcgct ataaactaaa
    21481 aaattacaca aaatcattcg cactgcatcg agacggaaag tgaatgaggg ccggggagcg
    21541 tactcgcagt acgtgacccg gtcgaatgag cgtagtcgtc gatgaggcag tgtgaaggat
    21601 gaagtgtaaa taaaaaagga agtgagcaat gcgaaaaagc ctgttagcta ttcttgcagt
    21661 ctcctcgttg gtattcagtt cggcgtcgtt tgctgctgat cttgaagaca atatggaaac
    21721 cctcaacgac aatttaaaag tggtcgaaaa agccgataac gcggcgcaag tcaaagacgc
    21781 gttaacgaag atgcgcgccg cagcgctgga tgcgcaaaaa gcaacgccgc cgaagctcga
    21841 agataaatca ccggacagcc cggaaatgaa agatttccgc cacggtttcg acattctggt
    21901 cggtcagatt gacgacgcgc tgaagctggc aaatgaaggt aaagtaaaag aagcgcaggc
    21961 tgctgcagag caactgaaaa cgacccgcaa ttcatatcac aagaagtatc gttaattcct
    22021 catttccctg ttgcctgcac tcaggtaaca gggaacttat catcgcaaat gatgcaccac
    22081 ctgattgctg ctgccgcgcc agatcaggga agggtctttt aaatcctgca caaatttgcc
    22141 gtcgaccagc acgttaatca aatcaacaac ctgcatttgc gcagcgttga gttcgtcgag
    22201 tttatagcct gtccacaccc agatgtcttt acccggacac tcggcgcgga tgcgttgtac
    22261 cagtttcaga atatccggca cgttttgcgg atgcagcgga tcgccgccgg agagggaaat
    22321 accctggcgt ttgatacgag tgtcattcag atcgttaatg atctggtctt ccattgcttt
    22381 agtaaatggc tgaccggaat ttacccgcca tgtgcttttg ttatagcaac cggggcattc
    22441 atgaacacac ccggagacaa acagggtgca gcgagtgccg ggaccgttga cgatgtcgac
    22501 aggatagtac tgatgataat tcattatttc gcctggctta tcagtccgcg tgtaggctgt
    22561 atcaggcaat caacgcctga tgcgacgctg tcgcgtctta tcaggcctac agccgttgcc
    22621 aaatgtaggc cggataaggc gtttacgccg catccggcaa tcaacgcggg atgcgacgct
    22681 ttcacatcac atccggcatt tgcttagtgc agatcaacct atctgcccat ttcccaaatg
    22741 tttaacgcgg cgcttaactt cttcctgctt gccggcgtta aacggacgtg catccgggct
    22801 acctaaatat ccgcacacgc ggcgagttac cgacacacgg gaggcgtcat ggttaccaca
    22861 tttcgggcag gtgaagcctt tgctggtgca ctcgaactca ccggtaaagc cacactcgta
    22921 gcactcatca atcggtgtat tggtgccgta atacggtaca tgctgatagc tgtaatccca
    22981 gacatcttcc agcgccttca ggttgtgctg aatgtttgga tactcgccgt agcaaatgaa
    23041 accaccgttc gccagcggcg ggtaaggcgc ttcaaagtcg atcttgtcgt acgggttcac
    23101 cttcttctcc acatcgaggt ggaaactgtt ggtgtagtaa cctttatcgg tcacgcccgg
    23161 caccacgcca aactcagcag tatcgagacg gcagaagcgg tcgcacaggt tttcactcgg
    23221 cgtgctgtag agactgaaac cataacccgt ttcttctttc cactgatcca ctgcctgacg
    23281 cagacgttca acaatggcga tacctttcgc gcggagctgc tcgttgtcgt aaacatgttc
    23341 gccgccgaac agcgcgttaa tggtttcgtg gatgccgatg taacccagcg aaatagacgc
    23401 acgaccgttt ttgaagattt cagaaatatc atcgtcagca ttgagacgca cgccacaagc
    23461 accttccata tagaggatcg gggccacgcg cgctttcacg ccttcgagac gagcgatacg
    23521 ggtcatcaga gccttacgtg ccagcaccag acgttcatcc agcagcttcc agaaggtggc
    23581 ttcatcgcct tttgcttcca gagcaatacg cggcaggttc aggctgatca cgccgaggtt
    23641 gttacgacca tcgtggatct gctcgccgtt ttcattttcc cacacgccga ggaagctgcg
    23701 gcagcccatc ggggttttaa acgaaccggt gactttcact acctgatcgt agttcaggat
    23761 atccggatac atgcgcttgc ttgcgcactc cagcgccagc tgtttgatgt cgtagttcgg
    23821 atcgcctttt ttatggttca gaccatcgcg aatcgcaaac accagtttcg ggaacaccgc
    23881 agttttacgg tttttaccga ggcctgcgat acggttacgc aggatagatt cctgaatcag
    23941 gcgcgactcc cagctggtgc ccagaccaaa accaaaggtt acaaacggcg tctgaccgtt
    24001 ggcggtgtgc agggtgttta cttcgtactc cagcgactgg aaggcatcgt agcactcttt
    24061 aatggtacga gagttagcgt agccttcggc gtccgggatg ttccactctt ctgcggtctt
    24121 gcgatgtttg ttgtagctgg cggtgacaaa cggtgccagc acttcatcga tacggttaat
    24181 ggtggtgccg ccataaatat ggctggcaac ctgagcaata atctgcgcag ttaccgcggt
    24241 tgccgtagag atcgacttcg gcggttcaat ctcggcgttc cccatcttaa agccctgggt
    24301 cagcatgcct ttcaggtcga tcaacatgca gttgaacatc gggaaaaacg gtgagtaatc
    24361 gagatcgtga tagtgaatat cgccacgctc atgtgcctgc accacgtcac gcggcagcag
    24421 gtgctgacgt gcatagtgtt tagccacgat cccggccagc aggtcgcgct gggttggaat
    24481 caccttgctg tctttgttgg cgttttcgtt gagtaacgag gcgttggtct gctcgaccag
    24541 accacggatc tcctgattca ggcgaccgcg tttttcacgt tcaatgtcgc gatcgtgacg
    24601 gtactcgatg taagcacgag ccagttgttt gtatggacct gacatcagct ggttttcaac
    24661 agcggtctgg atctcattga tatccacctg gttgcggccc tgcatctgct cgctgacaac
    24721 tgcagcaaca gtggcgcaat aatcggcatc atcgacttcc gctgctttag ctgcacgcag
    24781 aatcgcttct ttgatgcgct ctgatttaaa cggcactttg cagccgtctc gtttcatcac
    24841 atgcggtgtc atgatcgctc catattttta agaacaggtt atccacagaa attgggaaag
    24901 gcgttttccg gttttttcgt ttcgttttcc ggtgctttcc gcaatcgcca tccgcttatc
    24961 cacaacgccg cacctttttc gtggggagct gtagtagcaa ttatagtcga ttgatacaac
    25021 atattgggtt gggacgcatt ttaaagtcta tatatagtgc tttgcatcaa ggatgtttga
    25081 actttttttg atgtagctca aagtaaaaag cagagcgtac ggatgacggg tgctacagcg
    25141 ttatgtaaat tttttaatga atttgctggt tgaaaaatca acaaaaacaa catgctgaca
    25201 gacaaaaccc cggaatgacc ggcattaccg gggcttaggg aagatttact tctgtaacca
    25261 ccagacagcc tcaaaaggcc gtaaagtcat ggcacagggt tgtggtgagg cttcttcgta
    25321 gttatgcatc acaagctgcc agttgccgcg catttgccct agctgccagg gttggatctc
    25381 acggctaagg ttggcaatga ccagcaaggt ttgccccttc cattcacggc gatagcacca
    25441 caatacaggg ctgtttggca gcagatcctg gtaattgccc catgtcagga tggcttcctg
    25501 cttacgcagt gcgattaact tttggtaggt gtaaaacacc gaggactcat cggccagcgc
    25561 ggcttctacg ttgatttgtt gatagttatc gcccaggcca atccacggtt cgccagccgt
    25621 aaacccggca ttatcgccgt tgctccattg catgggcgtg cggctgttgt cacgggattt
    25681 actggcgagg attgccaata actcgtcggc atcacgccca tcgttgcgca gctcggcaaa
    25741 catattgagg ctctctacgt cgcgatagtc agtaatgcgc gtgaaatgcg ggttggtcat
    25801 gccaatctct tcgccctggt agatatacgg cgttccctgc atgccatgca gcaccatcgc
    25861 cagcattttt gccgcaggca cgcggtattc accttcatca ccaaagcgag aaacaatgcg
    25921 cggctgatcg tggttacacc agaacaaggc attcaatgct acgttgtgca ttccttgttg
    25981 ccagtggcgg aacaatgttt tcaacgccac aaagtcaggt ttagccagcg tccatttttc
    26041 accaccggga taatcgacct tcaggtgatg aaaattaaag gtcatcgaca attcactgcc
    26101 tgtcagtgcc gcgtatcgct ggcaatgctc aaggctggtg gaggacattt cacctacggt
    26161 cattaacccg cgtggcgtaa acacatcgcg gttcatctcg tgcaaaaact cgtgtgctcg
    26221 tggcccgtcg gtgtagaagc gacgcccgtc gccatccagg tcatcaggga aacgcgggtc
    26281 tttggagatc agattcacca catccaggcg caacccgtcg accccacgat cggcccagaa
    26341 ctcacagact tttttcagct ctgcgcgtac cgctggattc tcccagttga gatccgcctg
    26401 ttctggtgca aagagatgca aatagtactg ttcgctttcc gcatgccagc gccacgcact
    26461 accgccaaat tttgaacgcc agttgttcgg tggcgtttct ggttcgccat cgcgccagat
    26521 ataaaactgg cggtaagggc tttctttgtt cagcgcctcg cgaaaccagg catgttgggt
    26581 agaggtatgg ttaaacacca tatcgagaat gatacgaatc ccgcgcgatt ttgcctgcgt
    26641 caccagttcg tcaaaatcgt ccagcgtgcc gtaggtggga tcaatcgccg tatagttcgc
    26701 tacatcgtaa ccgttatcga cctgcggaga gacataaaag ggggttagcc agatggcatc
    26761 aacgcccagt ttatgcagat agtccaggcg ttggataacg ccacgtaaat cgccggtgcc
    26821 gctacccgtg gtgtcctgaa aactctttgg ataaatctgg tagataacgc cgttttgcca
    26881 ccagtgggga agattagtca ttacgttatt cctgcaaatg cgagggggcg caattgcgcc
    26941 ccgaagaaaa ttaaacaatg tccagcgtgc ccaggcggta tttccgctga tagataaacg
    27001 aggtgagtac aatcgggatg atgatggcga tagccattgc cagcgcaaac acctgccagt
    27061 agctcggttg aatcgagaga attcccggca ggccgcctac gccgatgcca ttcgccataa
    27121 cgccgttcag gccgcatagc aatcctgcca gaccagaacc aatcatcgcg cacagcatcg
    27181 ggaagcgata tttcaggttg atgccgtaca ttgcaggctc agtgacccca agccaggcgg
    27241 agatcgccgc aggcacggag atctcgcgtt cattgtgctt gcggctggaa atgataatgc
    27301 ctatcacggc ggagccctga gcgatattcg acagcgcaat cagcggccac actggcgtac
    27361 cacccatgct ttgaatcatc tgcaaatcaa tagcaagcgt ggtctggtgt acaccggtga
    27421 tcaccagcgg ggcgtacagg aagccaaaca atgccgcgcc aatcggagca aagctgccgg
    27481 tcatcaggtg acgtaccgca aaggcaacgc catcgccaat catgcgacca aacggaccaa
    27541 tcagcgcatg ggcgaggaac accgcgagga tcagcgaaca gacgggcacc accaccagat
    27601 agaggtaatc cggcacgatg cgtttaaggc gagtttcaat aacgcccagc gccagcccgg
    27661 ctaacagtgc cgggatcacc tgcgcctgat agcccacttt ggcgatgctg aacatgccaa
    27721 agtcccacac ttccggcagc tgctgcccga gcagataagc gttcatcagt tggggggaaa
    27781 ccagtgtcac gccaagcacg ataccaagga tcggcgtgcc gcccattttt ttcaccgctg
    27841 accagcaaat cccgaccggc aggtagaaga agatcgcttc accgatcaac cacagaaaat
    27901 cgtagatcgt ttgcagggaa gggtacattt gcgccagcgt ttgaccgttg ctcatgggca
    27961 aatcgccgat cacattgcga aaaccgagga tcaaaccgcc gctaatcaac gcgggcagca
    28021 acgggaagaa gatctctgcg aaatgagaga tcaactgctc atgccatttc atattctgcc
    28081 gggcggcttt ttttacctgc tctttatcaa cctgcgcctg tccggttgac gctatcagtg
    28141 cttgatagta atcacccacg ttggtgccaa tcaccacctg aaattgcccg gcattggtga
    28201 aacagccttt caccatgggg agttgctcaa tttctttcgg tctggcattg gccggttggt
    28261 tgaggacaaa gcgcaggcga gtaatacagt ggctcaccgt cgcaatattg ccgcgcccgc
    28321 cgaccagttc aatcaaccga tcgatatccg tttggtttat tttgctcatc ataaagcccc
    28381 atggcagatg acatttttgg ttggctgcag aatatattgc gcggaaaaat ttaaaaacgg
    28441 gaacgttccc gaaacgcagc gaagatcaca atttatcgtt caggaaacga tcaggacagg
    28501 ttggcgggga tgatgatttg ttgcggttcg ctgcgtccgg ttacctgcgc gatcaactgg
    28561 caagccgcct ggcgtccagc ttcggcgtaa ccaggatcta cggtgacgat ctccggatga
    28621 aggaatttca ttaacggcgt attaccgacg ctcgccagtt gcaaggtgtc gatgcgttgc
    28681 tcttgcaggt atttacttgc gccaagtgcc agcgtgtcgg ttgcgcacag taaggcggta
    28741 gtttcaggcg taatcacttt tgctacgttc tcatagcctt gcttcatagc aagccctggc
    28801 agggcggcaa cgggatgcag tttatgcgct ttgcagaacg ccaggtaggc ttcgtgacgt
    28861 cgcttaccgg ttgtcacgtc actgtgcggc acgccgagat aactgatatt acgatgcccc
    28921 tggtcataca gccgttgcat caggatcttg attgcccctt cgtcgtcata acagaccgaa
    28981 gcaaagcctt ttgcgtcacg cgccagcaga accagcgatg actgccagtg ggctaacatt
    29041 tcttctgtta tgccagtaaa accgaacagc actacgccgt cgatattacg ccgtttcagc
    29101 acccccaaat gttcggcaac taattgcggg gaaaactgac tttccatcat gattgggtcg
    29161 taaccttgtt catagaacgc tggcagcatg gtttgaacgg cgagattttc tgacaacgaa
    29221 tccagacggg taacaatgat ggcgaccact ttatcgcttt gcccacgcat agcgcgcgca
    29281 gaacgggaag gggaaaatcc atgctgattc atcactgctt caacacgctc gcgggtgcgc
    29341 tggctcacgc cgctttcgtt attcagcacc cgggaaactg tagatttccc cacgccgctt
    29401 aagcgcgcga tgtctttgat ggtcagccga ttttgcatcc tgttgtcctg taacgtgttg
    29461 tttaattatt tgagcctaac tttacccgtg cattcagcaa tgggtaaagt ctggtttatc
    29521 gttggtttag ttgccagcag gtattatatc gccatagatg ctacgaatat tattggattc
    29581 tccttattat ttgcggcgct tttttcactt accggaggtt atatggaacc tgatcccacg
    29641 cctctccctc gacggagatt aaaacttttc cggtaagccc gtcttttcac ggcgttaccg
    29701 gatgcgtaag gccgtgacgt tttaacgtcc ctgctcagct ttattacctt caggtaaggc
    29761 ttcgccacgc ctgaagacat ttctgtactg tttcagacag tgcggaggga ctccttatgt
    29821 ttaaagaaat ttttacccgg ctcattcgcc atttaccttc ccgtctggtt catcgtgatc
    29881 cattgcctgg cgcgcagcag acagtgaata cggtggtccc gccgtcctta agtgcgcatt
    29941 gcctgaaaat ggcggtgatg cccgaagaag aattgtggaa aacgttcgac acccatccgg
    30001 aagggttaaa tcaggcggaa gtggaatctg cccgcgaaca acatggtgaa aataaattac
    30061 ccgcacaaca accgtcgccg tggtgggtac atttatgggt ctgctatcgc aaccccttta
    30121 atattttact caccattctc ggcgctattt cttacgccac ggaagattta tttgccgcgg
    30181 gcgttatcgc gctaatggtc gctatttcaa cgttgctgaa ctttattcag gaagcacgtt
    30241 ccactaaagc ggcagatgcc ctgaaagcga tggtcagcaa taccgcgacg gtgctgcgcg
    30301 taattaacga caaaggcgaa aatggctggc tggagatccc gatcgaccag ctggtgcccg
    30361 gcgatattat aaaactggcg gcgggagata tgatcccggc agatttacgt atcttgcagg
    30421 cgcgggatct gttcgtcgct caggcgtcgt taaccggtga gtctctgccc gtggaaaaag
    30481 ccgctaccac tcgccagccg gagcacagca atccgctgga gtgcgacacg ctgtgtttta
    30541 tgggcaccac cgtggtgagc ggcacggcac aagcaatggt gattgctaca ggtgccaata
    30601 cctggtttgg tcaactggcg gggcgtgtta gtgagcagga aagcgagccg aatgcctttc
    30661 agcaagggat cagccgcgtc agtatgctgc tgattcgctt tatgctggtg atggcaccgg
    30721 tggtgctgtt aatcaatggc tacaccaaag gcgactggtg ggaagcggcg ctgtttgcgc
    30781 tttcggtcgc ggtaggccta acgccggaaa tgttgccgat gattgttacc tcgacgctgg
    30841 cgcgcggcgc agtaaagctg tcgaaacaga aagtgatcgt caaacatctg gatgctattc
    30901 agaactttgg cgcaatggat attctgtgca ctgataaaac cggcaccctg acacaggata
    30961 aaattgtgct ggagaatcat accgatatct ccggtaaaac cagcgaacgc gtgctgcata
    31021 gcgcgtggtt gaatagtcat tatcagaccg gacttaaaaa cctgctcgat acagcggtgc
    31081 tcgaaggtac ggatgaagag tcagcgcgct cgctggccag tcgttggcaa aaaatcgatg
    31141 agattccgtt tgatttcgag cgtcgccgga tgtcggtggt agtggcagaa aataccgagc
    31201 accatcagct ggtttgcaaa ggtgcattgc aggaaatcct caatgtgtgt tcgcaggtgc
    31261 gtcacaatgg cgagattgtg ccgctcgatg acatcatgct gcgtaagatt aagcgggtta
    31321 ctgatacgct gaatcgtcag gggctgcgcg tggttgcggt ggcgacgaaa tacctgccag
    31381 cgcgtgaagg agattaccag cgggcggatg aatccgacct gatcctcgaa ggatatattg
    31441 ctttccttga tccgccaaaa gagacaaccg ctccggcact gaaggcatta aaagcgagtg
    31501 gaattaccgt aaaaatcctt actggcgaca gtgagttagt tgcagcgaaa gtgtgccatg
    31561 aagtggggct ggacgcggga gaggtggtca ttggtagtga tattgaaacg ctatctgacg
    31621 acgaactggc aaatcttgca cagcgcacta cgctgtttgc ccgcctgacg ccgatgcata
    31681 aagaacgcat tgtgacctta ctgaagcgcg aagggcatgt ggttggcttt atgggcgatg
    31741 gtattaatga tgcgcccgcc ttacgcgctg cggatatcgg catttctgtg gacggcgcgg
    31801 tagatattgc ccgtgaagcg gctgatatca tcctgctgga aaaaagcctg atggtgctgg
    31861 aagagggggt tattgaggga cgtcgcactt tcgccaacat gctgaaatac atcaaaatga
    31921 cggcgagttc taacttcggt aatgtgttca gcgtgctggt ggcgagtgct ttcttgccct
    31981 tcctgccgat gttgccgtta cacttgctta ttcagaacct gctgtacgat gtgtcacagg
    32041 tggcgatccc gtttgataac gtcgacgacg agcaaattca aaagccgcag cgttggaatc
    32101 cggcggatct ggggcgcttt atgatcttct tcggaccgat cagctcgatc ttcgatattc
    32161 tgacgttttg cctgatgtgg tgggtattcc atgccaacac gccggaaacg caaacgctgt
    32221 tccagtcggg atggtttgtg gtgggcttac tgtcgcaaac gctgattgtg catatgatcc
    32281 gcacccgccg tgtgccgttt attcagagct gtgcatcgtg gccgttaatg atcatgaccg
    32341 tgatcgtgat gattgtcggg atcgcattgc cgttttcacc gctggccagt tatctgcaat
    32401 tacaggcgct gccgttaagc tatttcccgt ggctggttgc gattctggca gggtatatga
    32461 cattaaccca gttggtgaaa gggttctata gccgtcgtta cggctggcaa taaagaataa
    32521 actgggcacg atagccccga ttcagattgc agacaaagta agctttgttc atgccggatg
    32581 cggcgtaaat gccttatccg gccaacaaat tcaataaatt gcaggaatca cgtaggcctg
    32641 ataagcgtag cgcatcaggc gattttgcgt ttgtcatcag acttaatccg ggcatgatag
    32701 cccggatttc catcaagatt agcgacgaac agcgatcgct tcgatctcaa tcttcacgtc
    32761 tttcggcaga cgggcaactt caacgcaaga acgtgccggg aaggtggcgt tgtgttcggt
    32821 gaagaaggct tcgtaagtgg cgtttacggt tgcgaagtcg ttcagatctt ttacaaacac
    32881 ggtagtttta acgatgtcgc ccactttcag accagcggct tcgacgatcg cttttacgtt
    32941 atccagcgac tgacgtgcct gtgcagcgac gtctgccggt acttcgcccg ttttcggatt
    33001 taccgggatc tgaccggaag tgatgatcat attgcccaga tcaacgccct gtacgtaagg
    33061 accgatagct gccggtgcat tttccgtcgc gatagttttg ctcatgattt ctcctttatt
    33121 acagcggtaa aaagtctggc tcattatagg gagccagact tttattacca accgcaatta
    33181 attggccagc accacattat gggaaaactc tttttcacag tatttgcatt tgagcgcgat
    33241 atcattggcg cgttttcgca cggcaaagct ggaagaaacc ggttcggcat ggctgataca
    33301 gttgctgttc gggcagacca gcacattgtc gatgcgctcc ggcagacttg ggcgcgattt
    33361 acccaccact tcatagttgt cgatacggtt aaccgtggct tgcggcgcat acaatgccag
    33421 ttgatctacc tggtcttcac tcaaaaaggt attttcgatt ttgatcagat ctttgcggcc
    33481 catctcgcca gaaggcaggt tcagaccgat ggtgatgcgc tgatccgttt cggtcagctt
    33541 gaacagactc aacagcttaa aaccgatctg ggcggggata tggtcaatta ccgtgccgcg
    33601 tttaatagct tcaacctgca atttattatc gtgtgtcatc tctatttccc cttacagtac
    33661 cagatcgcga ttcagaacca gtgccagtaa cgcctggcga gcgaaaatcc cgttgcctgc
    33721 ctgctggaag taccaggcgt gtggcgtttt atcaacatcc gtcgcaatct catcaacacg
    33781 cggcagcgga tgaagtactt tcatattggc tttggcgttg tggagatcgc tggcgcgaag
    33841 aacaaactgc gctttcacgt tggcgtactc ggacgggtcc agacgctctt tttgcacgcg
    33901 ggtcatgtac aggatgtcaa cttccgccat cacttcttca atagagctgt gcagactcca
    33961 tgcgatccct ttttcatcaa gcatatccag aatgtattgc ggcattgcca gcgcgtccgg
    34021 cgcgatgaag taaaaacggt tgccgtcgaa cttcgctaac gcctgagtca gggagtgaac
    34081 ggtgcggcca tatttcaggt caccaaccat tgcgacgtgg agattgtcca gacgcccctg
    34141 ggtttcctga atggtgaata agtccagcaa ggtttgcgtc ggatgttggt tggagccatc
    34201 accggcattc agtaccggta cattgccgga aaactcggtg gccaggcgcg ccgcaccttc
    34261 ctgcggatga cgcatcacta tcgcatcgac gtaagtgctg ataaccgaaa tggtatcggc
    34321 cagcgtttcg ccctttttac ccagtgatgt attggcgctg tcggagaagc ccaccacgct
    34381 ggcacccagg cggtgcatgg aagtttcgaa agagaggcgg gtacgggtag aggcttcgaa
    34441 gaaacagctg gcaatgactt tgtgcttcaa cagctctggt tgcgggtttg ctttcagttt
    34501 cgccgctgtc gccagcacca gattaaggtc atcgcgacta aggtcgttta tggaaatgat
    34561 atgtttctga tatagcggat tagccatctt ttatctcctg acgcctgggc aaaaaaaagc
    34621 ccctcgattg aggggctggg aatgggtgat caacgggaag aaaaacggca ggccagcgtc
    34681 ttttttcaga cgcggtaaga caaaatgtcg aacacactga accatacatc ctcccggcaa
    34741 attgtccggc attatactca tcgtcagaag cggtgcaagc atttgatgca atattttgtc
    34801 agcgcaaacg gtttatttga attataagtc aaaatatatg catttttatt tactgtgatt
    34861 ctggagggga actgtcgttc ggtatcaata attgcagaca tttcctgctt ttttcttttt
    34921 cactttcacg caatcagata tgcattttat tccattcatc cgacttatag ggcggagtgt
    34981 gaaaacgaac ggctaacact attgcttaca gctcagggat gcgcgctatc actttaattt
    35041 caaaatcaaa gcctgccagc catgtaacgc ccaccgccgt ccaggttgga taaggtggcg
    35101 cgctaaatat ttcatttttc accgtcatga tgtcttcaaa ttgtttttct ggatcggtat
    35161 gaaagctcgt aacatcaatg atatcgtcaa aagtgcatcc cgcagctgcc agggtcgcat
    35221 gcaaattatc aaatgccagt ctgacttgtt gctgaaaatc gggttctggt gttccgtcct
    35281 ctcgacttcc tacttgcccg gaaacaaaca gcaaatcgcc ggaacgaata gccgcagaat
    35341 aacgatgttc tgcatacagt gaatgtcggc cagcagggaa aacagcggtt ctttctacca
    35401 tttggttatc ctcaagattt acgacatgaa cagaagattt atatttacca ggagccgctt
    35461 ttagcggacg acgtgagtaa acaaaaccca gacatcatgg gaaatggctg ggcttaattg
    35521 agcgtagtcg gttatgcgcc aaacgcgcca tcaatggtat gcatcgcgcc ggtaacaaaa
    35581 ctggcttctg gccctgctaa ccatgcgacc ataccagcga cctcttccgg ttgcccatgt
    35641 cttttgatag ccataaaact atgcaacata tcgcgcattg gcccgttggc gggattagcg
    35701 tcggtatcaa ttggccctgg ctggacgacg ttgatggtga tcccacgcgg tccaaaatca
    35761 cgggccagcc cgcgcgccat gccttgtagg gcagatttac tggcggcata agcagccatg
    35821 cctgcaacag gcatacgatc gccattcacg gagccgatga ttaagatgcg cccaccttcg
    35881 ggcatctgcc gggcggcttc aacagaggca tggtaaggag catgaatatt gattttgaaa
    35941 aggcgatcaa tatcgtcggc atttaattcc agggcatcgc caaagacgcc aatacctgca
    36001 tttaccacca ggatatccaa tgcgccgctc ttacgaacga catcaatgac agcgtctctg
    36061 tcagcactat ctgtaaatac tgctgtcgct cccgtctctt gtgccaggcg ttcagcggca
    36121 tctttcgacc ccgcataggt gaatcgtaca ttggccccat cggtgacgaa acgacgtacg
    36181 atagcggcac cgattccacg actgccaccg aggatgagaa ctgtcttacc tgtaaaagcg
    36241 cccataagga ctccttgatt tattatgtaa catgcattac aaaactgttt taactttctg
    36301 tcaacaagtt ttgtaatggt tactaaaaaa caatctcgcg ttccaggtcg tcccagacgt
    36361 ttcgctcctg agcaggcagt ctctgcggca aaagtgcttt ttcaccaaaa aggtttcgat
    36421 gctgtcagtg ttgctgaagt tactgattat cttggtatta accccccgag cctctacgcg
    36481 gcttttggca gtaaggctgg gttatttagc cgtgtactca atgaatatgt cggtacggaa
    36541 gctattccgc ttgtcgatat tcttcgtgat gatcgtccgg taggggagtg cctggctgag
    36601 gtattaaaag aagcggcgcg cagatatagc caaaacggcg gctgcgctgg ctgtatggtt
    36661 cttgaaggca ttcatagtca tgatccacaa gcgcgtgata ttgccgttca atattatcac
    36721 gccgcagaaa cggccattta tgactatatc gccaggctgc atccacaaag cgcacaatgt
    36781 gtgactgatt ttatgagtac cgtgatgtca gggctttctg cgaaggcacg agaggggcac
    36841 tccctcgaac aactctgtgc aactgctgca ctggcggggg aagcgataaa aactattctc
    36901 aaggagtgat gctggccttg atccgaaagg cgggaacgcg cctgccgata agttgtgata
    36961 agacaataat tcacgcgtta aggctggctg aattgatcat cttttcgtat aacggtggaa
    37021 atgaaacatt gttttaatta aggagtggaa aagatgatca tcggaaatat tcataatctt
    37081 cagccgtggc taccccagga gttacgccag gcgattgagc atatcaaagc acacgttacg
    37141 gcagaaacgc caaagggcaa gcacgatatc gaaggtaatc gtctgtttta tcttatctcg
    37201 gaagatatga ccgagccgta cgaagctcgc cgtgcggagt accatgcccg ctatctcgac
    37261 attcagattg tgttaaaagg tcaggaaggc atgaccttca gcacacaacc ggcaggtacg
    37321 ccggataccg actggttagc tgataaagac atcgcatttt tgccggaagg cgttgatgag
    37381 aaaacagtta tccttaatga aggtgatttt gttgtgtttt atccggggga agtgcataaa
    37441 ccgctgtgcg cagtgggtgc accagcccag gttcgcaaag cagtagtgaa gatgctgatg
    37501 gcgtgatgac ttttcgccgt aaataactcc aggtttacgg cgagtttgtg aaaagagcgt
    37561 tttttgatat ttttttgtga gtaaaatttg taatgcttag acgttcttat taactcaagg
    37621 agttcgtcat gagcaaaata tcagatttga attattctca acacattaca ttagccgaca
    37681 attttaaaca aaaaagtgaa gttttaaata cctggcgtgt tggaatgaat aattttgccc
    37741 gtaatgccgg gg
//
LOCUS       DBFIFI010000023        37302 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.023, whole genome shotgun sequence.
ACCESSION   DBFIFI010000023 DBFIFI010000000
VERSION     DBFIFI010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37302
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.023"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            84..1457
                     /gene="aroP"
                     /locus_tag="N7B39_001164"
     CDS             84..1457
                     /gene="aroP"
                     /locus_tag="N7B39_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HGU2421180.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVTVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAVVLVIMLMTPGMAISVYLIPVWLVVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(1500..2354)
                     /gene="ampE"
                     /locus_tag="N7B39_001165"
     CDS             complement(1500..2354)
                     /gene="ampE"
                     /locus_tag="N7B39_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HGU2421181.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFRTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(2351..2902)
                     /gene="ampD"
                     /locus_tag="N7B39_001166"
     CDS             complement(2351..2902)
                     /gene="ampD"
                     /locus_tag="N7B39_001166"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HGU2421182.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            2990..3883
                     /gene="nadC"
                     /locus_tag="N7B39_001167"
     CDS             2990..3883
                     /gene="nadC"
                     /locus_tag="N7B39_001167"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HGU2421183.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4086..4526
                     /gene="ppdD"
                     /locus_tag="N7B39_001168"
     CDS             4086..4526
                     /gene="ppdD"
                     /locus_tag="N7B39_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HGU2421184.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            4536..5921
                     /gene="gspE"
                     /locus_tag="N7B39_001169"
     CDS             4536..5921
                     /gene="gspE"
                     /locus_tag="N7B39_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HGU2421185.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLAGFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQKLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPVNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            5911..7113
                     /gene="hofC"
                     /locus_tag="N7B39_001170"
     CDS             5911..7113
                     /gene="hofC"
                     /locus_tag="N7B39_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HGU2421186.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQRAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGIMLTIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNIAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(7148..8191)
                     /gene="guaC"
                     /locus_tag="N7B39_001171"
     CDS             complement(7148..8191)
                     /gene="guaC"
                     /locus_tag="N7B39_001171"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HGU2421187.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIIS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(8347..8391)
                     /locus_tag="N7B39_001172"
     CDS             complement(8347..8391)
                     /locus_tag="N7B39_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2421188.1"
                     /translation="MIIKTLKMSARKRN"
     gene            8416..9036
                     /gene="coaE"
                     /locus_tag="N7B39_001173"
     CDS             8416..9036
                     /gene="coaE"
                     /locus_tag="N7B39_001173"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HGU2421189.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            9036..9779
                     /gene="zapD"
                     /locus_tag="N7B39_001174"
     CDS             9036..9779
                     /gene="zapD"
                     /locus_tag="N7B39_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HGU2421190.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDCQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            9789..9986
                     /gene="yacG"
                     /locus_tag="N7B39_001175"
     CDS             9789..9986
                     /gene="yacG"
                     /locus_tag="N7B39_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HGU2421191.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(10077..10475)
                     /gene="mutT"
                     /locus_tag="N7B39_001176"
     CDS             complement(10077..10475)
                     /gene="mutT"
                     /locus_tag="N7B39_001176"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HGU2421192.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     KWMSLVGLNADDFPPANEPVIAKLKRVYVG"
     gene            complement(10535..13240)
                     /gene="secA"
                     /locus_tag="N7B39_001177"
     CDS             complement(10535..13240)
                     /gene="secA"
                     /locus_tag="N7B39_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HGU2421193.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLAAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(13302..13814)
                     /gene="secM"
                     /locus_tag="N7B39_001178"
     CDS             complement(13302..13814)
                     /gene="secM"
                     /locus_tag="N7B39_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HGU2421194.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLS"
     gene            complement(14045..14962)
                     /gene="lpxC"
                     /locus_tag="N7B39_001179"
     CDS             complement(14045..14962)
                     /gene="lpxC"
                     /locus_tag="N7B39_001179"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HGU2421195.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(15063..16214)
                     /gene="ftsZ"
                     /locus_tag="N7B39_001180"
     CDS             complement(15063..16214)
                     /gene="ftsZ"
                     /locus_tag="N7B39_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HGU2421196.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(16275..17537)
                     /gene="ftsA"
                     /locus_tag="N7B39_001181"
     CDS             complement(16275..17537)
                     /gene="ftsA"
                     /locus_tag="N7B39_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HGU2421197.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(17534..18364)
                     /gene="ftsQ"
                     /locus_tag="N7B39_001182"
     CDS             complement(17534..18364)
                     /gene="ftsQ"
                     /locus_tag="N7B39_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HGU2421198.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(18366..19286)
                     /gene="ddlB"
                     /locus_tag="N7B39_001183"
     CDS             complement(18366..19286)
                     /gene="ddlB"
                     /locus_tag="N7B39_001183"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HGU2421199.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRVEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSVRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(19279..20754)
                     /gene="murC"
                     /locus_tag="N7B39_001184"
     CDS             complement(19279..20754)
                     /gene="murC"
                     /locus_tag="N7B39_001184"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HGU2421200.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(20808..21875)
                     /gene="murG"
                     /locus_tag="N7B39_001185"
     CDS             complement(20808..21875)
                     /gene="murG"
                     /locus_tag="N7B39_001185"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HGU2421201.1"
                     /translation="MSAQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(21872..23116)
                     /gene="ftsW"
                     /locus_tag="N7B39_001186"
     CDS             complement(21872..23116)
                     /gene="ftsW"
                     /locus_tag="N7B39_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HGU2421202.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(23116..24432)
                     /gene="murD"
                     /locus_tag="N7B39_001187"
     CDS             complement(23116..24432)
                     /gene="murD"
                     /locus_tag="N7B39_001187"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HGU2421203.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGVQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(24435..25517)
                     /gene="mraY"
                     /locus_tag="N7B39_001188"
     CDS             complement(24435..25517)
                     /gene="mraY"
                     /locus_tag="N7B39_001188"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HGU2421204.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLVGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(25511..26869)
                     /gene="murF"
                     /locus_tag="N7B39_001189"
     CDS             complement(25511..26869)
                     /gene="murF"
                     /locus_tag="N7B39_001189"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HGU2421205.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIVWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFAEKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(26866..28353)
                     /gene="murE"
                     /locus_tag="N7B39_001190"
     CDS             complement(26866..28353)
                     /gene="murE"
                     /locus_tag="N7B39_001190"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HGU2421206.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKGEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQDATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEDRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(28340..30106)
                     /gene="ftsI"
                     /locus_tag="N7B39_001191"
     CDS             complement(28340..30106)
                     /gene="ftsI"
                     /locus_tag="N7B39_001191"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HGU2421207.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(30122..30487)
                     /gene="ftsL"
                     /locus_tag="N7B39_001192"
     CDS             complement(30122..30487)
                     /gene="ftsL"
                     /locus_tag="N7B39_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HGU2421208.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(30484..31425)
                     /gene="rsmH"
                     /locus_tag="N7B39_001193"
     CDS             complement(30484..31425)
                     /gene="rsmH"
                     /locus_tag="N7B39_001193"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HGU2421209.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(31427..31885)
                     /gene="mraZ"
                     /locus_tag="N7B39_001194"
     CDS             complement(31427..31885)
                     /gene="mraZ"
                     /locus_tag="N7B39_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HGU2421210.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(32487..33491)
                     /gene="cra"
                     /locus_tag="N7B39_001195"
     CDS             complement(32487..33491)
                     /gene="cra"
                     /locus_tag="N7B39_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HGU2421211.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(33573..33659)
                     /locus_tag="N7B39_001196"
     CDS             complement(33573..33659)
                     /locus_tag="N7B39_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HGU2421212.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(33671..34162)
                     /gene="ilvN"
                     /locus_tag="N7B39_001197"
     CDS             complement(33671..34162)
                     /gene="ilvN"
                     /locus_tag="N7B39_001197"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HGU2421213.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLYKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(34165..35889)
                     /gene="ilvI"
                     /locus_tag="N7B39_001198"
     CDS             complement(34165..35889)
                     /gene="ilvI"
                     /locus_tag="N7B39_001198"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HGU2421214.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGIG
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGGAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPQELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            complement(36207..37151)
                     /gene="leuO"
                     /locus_tag="N7B39_001199"
     CDS             complement(36207..37151)
                     /gene="leuO"
                     /locus_tag="N7B39_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308107.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HGU2421215.1"
                     /translation="MPEVQTDHPETAEFSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKLNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT         9020 a        10401 c         9104 g         8777 t
ORIGIN      
        1 acaaattaat acaacaaacg gaattgcaaa cttacacacg catcactgcg tagatcaaaa
       61 aaacaatcac cgcacgaggt ttcatgatgg aaggtcaaca gcacggcgag cagctaaagc
      121 gcggccttaa aaaccgccat attcagctta tcgcgctggg tggcgcgata gggacagggt
      181 tattcctggg tagcgcctcc gtaatacagt ccgcagggcc agggattatc ctgggttacg
      241 ccattgctgg ttttatcgcc tttctgatca tgcgtcagct gggtgaaatg gtggtcgaag
      301 aacctgtcgc aggctccttt agccactttg cttataaata ctggggcagc tttgctggct
      361 tcgcttctgg ctggaactac tgggtactgt acgttttagt tgccatggca gagctgactg
      421 ctgtgggtaa atacattcag ttctggtatc cggaaatccc aacctgggtt tctgccgccg
      481 tgttctttgt ggtgattaac gccatcaacc tgaccaacgt aacagtgttt ggtgagatgg
      541 agttctggtt tgccattatc aaagttattg cggtagtagc gatgatcatc ttcggcggct
      601 ggctgctgtt cagtggtaac ggcggtccgc aggcaagcgt tagcaacctg tgggatcagg
      661 gcggtttcct gccgcacggc ttcaccgggc tggtgatgat gatggcgatt atcatgttct
      721 cgttcggtgg tctggaactg gtggggatca ccgcagcaga agctgataac ccggagcaaa
      781 gtatcccgaa agcaactaac caggttatct accgcatcct gattttctat attggttcgt
      841 tagccgttct gctctcactg atgccgtgga cccgcgttac cgccgatacc agtccgtttg
      901 tgctgatctt ccacgagtta ggcgatacct ttgtggcgaa tgcgctgaac atcgtggtac
      961 tgactgcggc gctctccgtg tacaacagct gcgtatattg caacagccgt atgctgtttg
     1021 gtctggcaca acagggtaac gcgccaaaag cgctggcgtc tgtcgataaa cgcggcgtac
     1081 cggttaacac cattctggtg tctgcgctgg ttacagcatt gtgcgtattg attaactatc
     1141 ttgctccgga atccgcattt ggcctgttaa tggcactggt ggtatccgca ctggtgatca
     1201 actgggcgat gatcagtctg gcgcatatga agttccgtcg cgccaagcag gaacaaggcg
     1261 tggtaactcg cttccctgct ctgctttatc cgctgggtaa ctggatctgc ctgctgttta
     1321 tggcggtggt actggtgatt atgctgatga ccccaggaat ggcgatttcg gtatacctga
     1381 tcccggtatg gctggtggtg ttaggtatcg gctatctgtt taaagagaaa accgcaaaag
     1441 ccgtaaaagc acattaatct ctctacgccc tcacccgtac agggtgaggg caataatctt
     1501 tacaccaacg ccccgtaaat cgtcagtagc gcaatcacca ccacgaccac gaacgaggtt
     1561 ttcttcgcca ttgaaaccgc tgccttcggc gtctccacct tatcgacatg cggttcacgc
     1621 gccagagaga actgcgccag acgcgttaat acctgatatt gcgaagtacg gaaatcaccc
     1681 agcgaagcaa accaggccgg taacgctttc tcaccatgac cgatcaaggc atataccaca
     1741 cctgcaagac gaaccggcac ccaatccagt acatgaagca ctgcatcaat gccggactgt
     1801 aaacgatgat gcggcgtctg gtatcgcgcc agccagtatt gccatgcacg caaaaacgca
     1861 taccccatca gcgtaacggg tccccaggtt ccccccacaa tcagccagaa cagcggtgca
     1921 agataaaaac gaaagttaat ccacagcaat gcattttgca gctcacgcaa atactcacgt
     1981 tcgtcgcagc ctgccgggac gccgtgaatc atggtgagtt cgccagccat cgtggcacgg
     2041 gcatggctat cattacgtga agcagctgtc agataagcat gataatgaag acgaacttta
     2101 cctgcgccaa tacacagcaa accaatcagc agccacacca gcagcgtggg aacgttgaac
     2161 aatactccct gcaatgcgcg taacagtaaa aaagtcacgc ccatcgcaat aatggtcatg
     2221 cctaacgtgc gcccgagaga aaaatgtttc acccgccgaa agaacgcttc aagacgatga
     2281 tcaagctgcc agtgctcgcc caacttaaac aggcgctcga aaattaacac cagtaaggtt
     2341 gtaaatagcg tcatgttgtc tccttgctga ccagcgcacg aaaccgtgcc caatcaaatg
     2401 caggaccggg atcggttttc cgatccggcg caatatcaca atggcccgtc atgttattag
     2461 cgatatccgg atagcaatca atcagtgccc gcgtaaccgc cgcaagctgt tgatactgcg
     2521 catcggtata cgccagcgta tcggtgcctt caagctcaat cccaatagaa aaatcattgc
     2581 agcgttcgcg cccctgatac tgagagactc ccgcatgcca tgcacgttta tcgaaaggaa
     2641 catactggac tatttcacca tcacggcgaa tcaaacagtg agcggagacg cgcaaatggg
     2701 cgatctcagc aaagaaagga tgtgcctgcg gatcaatagt tccagtgaat aatgcgtcga
     2761 tccacggacc gccaaactcg cctggcggca ggctaatatt gtgcaccacc agcagggtgg
     2821 gtgtttcgtc atccgggcgg caatcgtaat gtggtgaggg aacgcggcgc gcgccaacca
     2881 gccacccctg ttctaacaac atgcaggatc tccttatatg tggtgctaat acccggttca
     2941 gagtagcatg tttctacctt atgattcgtt agctatctgg agttttaaca tgccgcctcg
     3001 ccgctataac cctgacaccc gacgtgacga gctgctggaa cgcattaatc tcgatatccc
     3061 cggcgcggtg gcccaggcgc tgcgggaaga tttaggcgga acagtcgatg ccaacaatga
     3121 cattacggca aaacttttac cggaaaattc tcgctctcat gccacggtga tcacccgcga
     3181 gaatggcgta ttttgcggca aacgctgggt tgaagaggtg tttattcaac tggcaggcga
     3241 cgatgtcacc ataatctggc atgtggatga cggcgatgtc atcaacgcca atcaaccctt
     3301 gttcgaactt gaaggcccat cccgcgtgct gttaacgggc gaacgcactg cgcttaattt
     3361 tgtgcaaacc ctttcaggag ttgccagtaa ggtacgccac tatgtcgaat tactggaagg
     3421 caccaacacg cagttgttgg atacgcgtaa aaccttaccc ggtctgcgtt cagcgctgaa
     3481 atacgcggta ctttgcggcg gcggagcgaa tcaccgtctg gggctttctg atgccttcct
     3541 gatcaaagaa aaccatatta ttgcctccgg ctcagtgcgc caggcggtcg aaaaagcgtc
     3601 ctggctgcac ccggatgcgc cagtagaagt cgaagtagag aatctggaag aacttgatga
     3661 agccctgaaa gcaggagccg atatcatcat gctggataac ttcgaaacag aacagatgcg
     3721 cgaagccgtc aaacgcacca acggcaaggc gctactggaa gtgtctggca acgtcactga
     3781 caaaacactg cgcgaatttg ccgaaacggg cgtggacttt atctccgtcg gtgcgctaac
     3841 taagcacgta caggcactcg acctttcaat gcgttttcgc tagttgctga cagatttatc
     3901 cgctccatag tgggcggata atctcccctt ccccaacctt cttcgtaacg cctcgcaaat
     3961 ttcttgtctt tcgctgcaac gaaataaaaa acctcggaag cagctttccc gtttcgtttt
     4021 ttgcccctcg cctgtgctga tggcgtttgc caaagtagca ccaaccaaat caaggagcga
     4081 aacagatgga caagcaacgc ggttttacac ttatcgaact gatggtggtt attggcatca
     4141 ttgccatttt aagcgccatt ggtattcccg cttatcaaaa ctacctgcgc aaagccgcac
     4201 tcaccgacat gctacaaacc tttgtgcctt accgtaccgc cgtagagttg tgcgcgctgg
     4261 aacatggtgg attagatacc tgcgacggtg gcagcaatgg cattccctcg cctaccacca
     4321 cccgctatgt ttcagccatg agtgtggcaa agggcgtggt gtcgctgacc gggcaagaaa
     4381 gtctcaatgg gctaagcgtc gtcatgacgc caggttggga taacgcaaac ggcgtcaccg
     4441 gctgggcgcg caactgcaat attcaaagtg acagcgcatt gcagcaagcc tgcgaagatg
     4501 tcttccgctt tgatgacgcc aactaaggag cggcaatgaa tattccacag ctcacggccc
     4561 tgtgcctgcg ttatcaggga gtcttgctgg atgccagcga agaagtggtt catgttgcgg
     4621 tggtcgatgc cccctcacat gagttgctgg acgcattgca tttcgctacc accaaacgta
     4681 ttgagatcac ctgctggacg cgccaacaaa tggaaggtca cgccagtcgc acacaacaga
     4741 cattgcccgt agctgttcag gagaagcatc agcccaaagc agagttgctg actcgaacgt
     4801 tacaatctgc gctggaacaa cgcgcgtctg atattcatat cgaaccagcg gacaatgcct
     4861 accgcatccg cttgcgtatc gacggcgtat tgcatccttt accggatgtt tcaccggatg
     4921 ccggagtcgc attaaccgcc agattaaaag tgctgggaaa cctggatatt gcggaacatc
     4981 gcctgccgca ggacgggcaa ttcactgtcg aactggcagg aaacgccgtc tcatttcgta
     5041 ttgcgacctt accatgtcgg ggtggtgaaa aggtggtatt aaggttgtta cagcaggtga
     5101 gccaggcact ggatgttaac acgctgggaa tgcagccgtt acaactggcg ggctttgctc
     5161 atgccttgca acaaccacag ggactggtgc tggtaactgg ccctaccggc agcggcaaaa
     5221 cggtcacgct ttatagtgcc ctgcaaaagc tgaataccgc tgacattaat atttgtagcg
     5281 tcgaagatcc ggttgagatc cccatagccg gactaaacca gacgcaaatc catccgcgtg
     5341 ccggactcac ctttcagggc gttttgcgtg cgttattgcg ccaggatcct gacgtcatca
     5401 tgatcggaga gatccgcgat ggcgaaacgg cagaaattgc cattaaagcc gcgcaaaccg
     5461 gtcacctggt gttgtctacc ctacacacta attccacctg cgaaacgctg gtacgtttac
     5521 agcaaatggg ggtcgcccgc tggatgctat catcggcgct tacgctggta atagcccagc
     5581 gtctggtacg caaactttgc ccacattgtc gccggcagca aggggagccc atccacattc
     5641 cagtcaatgt atggccgtcg ccgctgcccc actggcaagc acccggttgt gtacattgct
     5701 accacggttt ttatggtcgc actgccttat ttgaagttct gcccataaca ccggtcatac
     5761 gtcagcttat ttccgctaat accgacgttg aatcgctgga aacgcacgcc cgacaggcgg
     5821 gtatgcgaac gctttttgaa aacggctgcc tggccgtgga gcaaggctta accacctttg
     5881 aagagttaat ccgcgtattg gggatgccgc atggcgagta agcaactctg gcgctggcat
     5941 ggcataaccg gcgacggcaa tgcgcaagat gggatgctat gggcagagag ccgtacttta
     6001 ctgcttatgg cactacagca acagatggtt accccactaa gcctgaagcg aattgctatc
     6061 aattctgcgc agtggcgagg agataaaagc gcggaagtca ttcatcaact ggcgacgcta
     6121 cttaaagccg ggttaacgct ttctgaaggg ctggcactgc tggcggaaca gcatcccagt
     6181 aagcaatggc aagcgttgct gcaatctctg gcacacgatc tcgaacaggg cattgccttt
     6241 tccaatgcct tattaccctg gtcagaggta tttccgccgc tctatcaggc gatgatccgc
     6301 acgggtgaac tgaccggtaa gctggatgaa tgctgctttg aactggcgcg tcagcaaaga
     6361 gcccagcgtc agttgaccga caaagtaaaa tcagcgttac gttatcccat catcatttta
     6421 gcgatggcaa tcatggtggt tgtggcaatg ctgcattttg ttctgccgga gtttgccgct
     6481 atctataaga ccttcaacac cccactaccg gcactaacgc aggggatcat gacgctggca
     6541 gactttagtg gcgaatgggg ctggctgctg gtgttgttcg gcattatgct gacgatagcc
     6601 aataagttgc tgatgcgccg cccgacctgg cttatcgcgc ggcagaaatt gctgttacgc
     6661 atcccgatta tggggtcact gatgcgggga caaaaactca cgcagatctt tacgattctg
     6721 gcgctgactc aaagtgcagg cattactttt ttacagggcg tagagagcgt cagagaaaca
     6781 atgcgctgcc cgtactgggt gcaacttctg acacaaatcc agcacgatat cagtaacggt
     6841 catcccatct ggctggcgtt aaaaaatgcc ggtgagttta gcccgctctg tttgcaatta
     6901 gtgagaacgg gagaggcctc cggctcgctg gatctcatgt tagacaacat cgcccatcat
     6961 catcgggata acacaatggc gctggcggat aacctagcag ccttactgga accggcgttg
     7021 ctgatcataa cgggaggaat tatcggtacg ctggtggtgg cgatgtatct gccaattttc
     7081 catttaggcg atgcgatgag tgggatggga taacgctggc gtgttgctcc acaccagcgt
     7141 tggaagatta caggttgttg aagatgcggt tttcttgttc ctgcacacgg ataaatgtgg
     7201 tgcgcttggt cagttctttc aggcgtgaag ccccaacgta tgtacaagct gaacgcaggc
     7261 cgcccaaaat atcacgcgcg gtattttcaa ccgggcctcg cagcggcagc ttaacggttt
     7321 taccttctgc tgcgcgatat tcagcaacgc cgccaacgtg acgtttcatc gcagactcgg
     7381 agctcatgcc gtagaacagc ataaatttct cgccgttctc ctcaacgatg cgaccgccgc
     7441 tctcttcgtg gcccgccagc atgccgccaa gcatgacgaa atcggcaccg ccaccaaagg
     7501 ctttcgccac atcgcccggc gtggtgcagc caccatcgct gatgatcatt ccgcccagac
     7561 cgtgcgcagc atcggcacat tcgattaccg cagaaagttg cggataaccg acgcctgttt
     7621 tgacgcgagt tgtacaaaca gaacctgggc caatgccaac tttaacgata tcggcacctg
     7681 agaggataag ctcctcacac atttcaccag tcactacgtt accagcacaa atggttttgg
     7741 tcggccacgc ttcacgcgct ttcgcaacga actgcacgaa gtgttcggaa taaccattcg
     7801 ccacgtcaat acaaacgaag tttaatgccg ggttcaggtc gagaatctgt ttagtttttt
     7861 cgaaatccgc atcagacgta ccggtagaaa ccatcacatg tttcagcaca tcagcggaag
     7921 aattgttgat aaacgcttgc cactcttcga cagaatagtg tttatgcaca gcagtcaaaa
     7981 tatcaaaaga agccagcgca gaggccatag aaaatgtgcc tacggtgtcc atatttgcgg
     8041 cgataatcgg cacgccggac cagctctgac ctgaatgttt gaaggtgaat tgacgttcca
     8101 gttcaacatc ggaacggctt ttaagagtgg agcgtttagg gcggatgaga acgtctttaa
     8161 aacctaactt cagatcttct tcaatacgca tgtgcggatt cctggggtta atggctataa
     8221 gggtaagagc gcaactccag tgacgctatc atacgcacta atcaatgcgg cgcaagactg
     8281 cgaaattgtc tttttttacg ctacaatccc ataaatttac ctggtaaata gtgagcgaac
     8341 gtttcatcaa tttcgctttc gtgcgctcat ttttaacgtt ttgataatca tgatgaattc
     8401 ccgggaatat aaattatgag gtatatagtt gccttaacgg gaggcattgg cagtggcaag
     8461 agtaccgttg ccaatgcgtt tgctgatctc ggaattaacg tcattgatgc cgatattatt
     8521 gcgcgtcagg tggttgaacc aggtgcacct gcgctacatg ccattgctga tcactttggc
     8581 gctaatatga ttgctgctga tggaacattg cagcgccggg ccttgcgcga gcggatcttc
     8641 gccaacccgg aagagaaaaa ctggcttaac gccctgctgc atccgctgat tcagcaagag
     8701 acgcaacacc agatccagca agctacttcc ccctatgtac tgtgggttgt gccattgctg
     8761 gtagaaaact cactgtataa aaaagcgaat cgagtgctgg tggtggatgt cagcccagaa
     8821 acgcaactta agcgcaccat gcagcgcgat gatgtaactc gcgagcatgt cgaacaaatc
     8881 cttgctgctc aggcaacgcg cgaagcccgc cttgccgtgg cagatgacgt cattgataat
     8941 aacggcgcac cggatgctat cgcatcggat gttgcccgcc tgcacgcaca ctatttgcag
     9001 cttgcgtcgc agtttgtctc acaggaaaaa ccgtaatgca gacccaggtc ctttttgaac
     9061 atccactaaa tgaaaaaatg cgtacatggc tgcgcattga gtttttgatt cagcaactca
     9121 ccgttaattt acccatcgtt gaccacgctg gcgcgctgca tttcttccgt aatgttagtg
     9181 aattactgga tgttttcgag cgcggcgaag tccgcactga gctgttgaaa gaacttgacc
     9241 ggcagcaacg taaactccag acctggattg gcgtgcctgg cgtggaccag agccgtattg
     9301 aagcattaat tcagcagtta aaagcggcgg ggagcgtatt aatttccgcg ccgcgtatcg
     9361 ggcaatttct gcgtgaagat cgtttgattg ctctggtgcg tcagcgactg agcatcccag
     9421 gcggctgttg cagctttgat ttacctacat tgcacatttg gctgcatcta ccccaggcgc
     9481 agcgcgactg ccaggtagaa acctggattg ccagcctgaa cccgctcacc caggcactta
     9541 ccatggtgct ggatttaatt cgccagtcgg cccccttccg taaacaaacc agcctgaatg
     9601 gtttttatca ggataacggt ggcgatgccg acttgctgcg cctgaatctg tcgctcgatt
     9661 cacagcttta tccgcaaatt tccggacata agagccgttt tgccattcgt tttatgccgc
     9721 tggacactga aaacggacag gtaccggaac gtctggattt cgaactggcc tgttgctaag
     9781 gagtaaaaat gtcagaaact attacggtga attgcccaac ctgcgggaaa acggtggtgt
     9841 ggggtgaaat cagcccgttt cggccatttt gctccaaacg ttgtcagctg atcgacctcg
     9901 gagaatgggc tgctgaagaa aaacgaatcc ccagcagtgg cgatctttcc gaaagcgatg
     9961 actggagcga agagccaaag cagtgacatt tgtctgatgc cgcacgtagg cctgataaga
    10021 cgcggacagc gtcgcatcag gcatcttgtg caaatgtcgg atgcggcgtg aacgccttat
    10081 ccgacataca cacgcttaag cttcgcaatt accggttcat tggctggcgg aaaatcatcg
    10141 gcattaagac cgactagcga catccactta ccgggttgcc cttctttacc ccacggctcc
    10201 ccttcccagc tttcgaccag ccagaaccac agagttatat gcctgtccgg gaattcatat
    10261 tccagttttt caaatagcga aaaatgttgt ggggtaatcc cgacttcttc ctgaagttca
    10321 cgcaccaccg cctgttccgg cgtttcaccc atttcaattt taccgccggg aaactccagt
    10381 ttattcgcca tgtgcgcatc tgctgcgcga cgcgttataa agatttcatt gttctcgttg
    10441 cgaataatac ctaccgcaat ttgcagcttt ttcattgtct taaacctata aaaaaggcgc
    10501 agaatcctgc gccttttact tcaacagtta gcttttattg caggcggcca tggcactgct
    10561 tgtatttttt accagaaccg cacgggcaag gatcgttacg tcctactttg cgctcaccgg
    10621 tttgcgccgc cagtgcagct gcggctgcag agtcgtcatc ctgatggcta agctgctgca
    10681 tttgcgctaa acgctcggct tccatacgac gctgttgttc cagctcctca acctcttcag
    10741 gcatacgtac ctgaactttg ctcagcgtac tgataacttc atatttcaac gactccagca
    10801 tcgctgcaaa catggagaac gattcacgtt tgtattcctg cttcggatct ttctgtgcat
    10861 agccacgcag gtggataccc tgacgcagat agtccatcgc tgccaggtgc tctttccaca
    10921 gggagtcaag cgtttgcagc atgacgcctt tctcgaagtg acgcatcatc tcagcaccaa
    10981 ccacttcttc tttacgctga tacacttcga tggactgcgc cagaatgcgc tcacgcagcg
    11041 tctcttcatg cagttctggt tctttatcca gccactcagc aattggtaaa tcgaggtcga
    11101 aatcgttctt cagacgttcc tgcagccccg gaatatccca catttcttcc agcgactgtg
    11161 gcggaatgta ggcatcaatg gtcgctttga acacatcttc acgaatgctg ttaatggttt
    11221 cgctcacatc gctgacatcc agcaattcgt tacgctggga gtaaatggcg cgacgctgat
    11281 cgttagccac gtcatcatat tccagcagtt gcttacgaat gtcgaagtta cgactttcaa
    11341 ctttacgctg ggcgttggca atcgctttag tcacccacgg gtgttcaatg gcttcgcctg
    11401 gcttcatacc cagtttacgc atcatgccgg atactcggtc ggaagcaaaa atacgcatca
    11461 gcgcatcttc catcgacagg tagaaacggg aagaaccagc atccccctga cgaccagaac
    11521 gaccgcgcaa ctggttatcg atacgacggg attcgtgacg ctcggtaccg atgatatgca
    11581 ggccacctgc tgccagtacc gcatcgtgac gtacctgcca gtcggcttta attttttcaa
    11641 tttgctctgc ggtcggattt tccagcgcgg caacttctgc ctgccagcta ccaccgagca
    11701 caatatctgt accacgaccc gccatgttgg tcgcgatagt caccgcagcc ggataacctg
    11761 cctgagcaac aatcgccgct tcgttggcgt ggaatttggc gttcaggaca ttgtgcttaa
    11821 taccggcttt ggtcagttcg tttgacacca gctccgattt ttcgatggag atagtaccca
    11881 ccagcaccgg ctggcctttc gcagtacgtt ctttgatatc ttcaatgatc gcctgaattt
    11941 tttccgcttc agtcatgtag accaggtccg gcagatcttt acgaatcatt ggacggttgg
    12001 tcggaacaac gacggtatcc agtttgtaga tggagctaaa ttcgaaagct tcggtatcag
    12061 cagtaccggt catccccgcc agtttttcat acagacggaa gtagttctgg aaggtgatcg
    12121 aagccagcgt ttggttttcg ttctggatct gcacaccttc tttcgcttcc acagcctggt
    12181 gcagaccatc ggaccagcga cggccctgca tggtacgacc ggtgtgttcg tcaacgatga
    12241 taacttcacc atctttaacg atgtagtcga cgtcacgggt aaacagcgca tgagcgcgca
    12301 gcgccgccgt tacgtggtgc atcagcatga tgttggccgg agagtacaga gactcccctt
    12361 catccatgat gccctctttc accagcagtt cttcaatcag caccagacca cgttcggtca
    12421 ggttcacctg gcgagatttt tcgtccaccg agaagtggcc ttcgccctgg aaggtttcgg
    12481 agtcttcttt ttcctgacgg atcaggtgcg gaataatttt attcacgcgt ttatacattt
    12541 ccgagctgtc ttctgccggg ccggaaatga tcagcggtgt acgcgcttca tcgatcagga
    12601 tggagtccac ttcgtccacc agcgcatagt gcagtttacg ctgtacacgt tcttcagggc
    12661 tgaacgccat attgtcgcgc aggtagtcaa agccgtattc gttgttcgta ccgtaagtga
    12721 tgtcagctgc gtaagcttcg cgctttgccg gtgctggcat gcccggcagg ttgataccga
    12781 cagtcaggcc aaggaattca aacagcggac ggttgttttc ggcgtcacgt tgcgccaggt
    12841 agtcgttgac ggtaactaca tgcacgcctt taccggttag tgcgttcaga taagcaggca
    12901 gcgttgcggt cagggtttta ccttcaccgg tacgcatttc ggcgatgcag cgttcgttaa
    12961 gaaccatacc gccgagtaac tgaacgtcga agtgacgcat accaaagacg cgcttacttg
    13021 cctcacgtac cacggcgaaa gcttccggga tcagattttc cagcacttcg cctttttcca
    13081 gacgtgcacg aaactctgcg gttttccctt tcagttcttc gtcggagagt ttttccatct
    13141 ccggttccat ggcattgatg atgttgacca ctttgcgcat ccggcgcagg gtgcgatcgt
    13201 tacgactacc gaaaacttta gttaataatt tgattagcat aataaaatct caaacgcccc
    13261 gcgttgcgga gttaataaaa tgaagtaaag gtttattgtt gttagctgag gcgttgaggg
    13321 ccagcacgga tgccttgcgc ctggcttatc cagacgggcg tgctgaattt tgcttgtggg
    13381 gtaaaatgcg cataatcaat gcgataaccc ttttcagacg gcgtgccttc ctgggtcagc
    13441 agcgcgctga gcgtatccag taatgcaaga tgttgcgcct gaagaggcaa agattcttca
    13501 gcaacgggca gcgtttgcgg tgccattgcg aaggaaagat gacggattac cgtgcgaatg
    13561 gcatgttgat gccagtaatc tacggaatag ttcgaattcg ggcggcgcgt gttcgcttcc
    13621 agcaaggcca attgaccaaa gttaactttg gctgaaggct cgtggttgcg ggttgtcgct
    13681 tttgcgggcg cgtttggttc ggcggcgttg ctgagcgcag gcaaacctaa actcgccgca
    13741 accatcccta ataagagatg cggccagaag tagcgtttac caaactgtcg ccagcgcgtc
    13801 agtattccac tcacgttatt gccatcccga tcgtattcaa acgtatttag cgcacaaatc
    13861 ttatcattaa agcctgaagt ccacagcacg aatgaagata agttgtccgt aaaagtgctt
    13921 aagaaaacag cgttcgcacc gttttttata gctcttataa aattcaaaaa aaaacgactg
    13981 gttcacctgg ccggagagag tgccagattt gccagtcgaa ttttatacga cagtataaat
    14041 gtcgttatgc cagtacggct gaaggcgctt tgaaggccaa cggcagttct gcgtcgtcct
    14101 ggaaggtcac atattcccag gcttcctgtt tcgccaggac agcctgcagc agtttgttat
    14161 tcagtgcatg accggattta taagcggtaa atgcaccaat aatattgtga ccacacatga
    14221 acaagtcacc gatcgcatcg agcattttgt gacgcacaaa ttcgtcttca aaacgcaggc
    14281 cgtcttcgtt cagtacgcga taatcgtcaa caacgatggc acaatcgaag ctgccgccca
    14341 ggcacaaacc acgggactgc agatattcga tatcacgcat gaaaccgaac gtacgcgcac
    14401 ggctgatctg gcgcataaac gcatcagcgg agaagttcat cgcatagcgc tggttgctgg
    14461 aatcaatagc cggatggtta aaatcgatgg tgaaatccag cgaaaaacca ttgtacggct
    14521 taaattcagc ccacttatcg ccatcttcga cacgaacagt ctctttgatg cgaacaaatt
    14581 ttttggcgca gttcaactcg tcgataccgg cgtcaagcag caggtataca aacggagcgg
    14641 cgctgccgtc catgatcggg atttccggcg cgttaacttc gataacaatg ttatcgatgc
    14701 ccaagcccgc gagagcagca ttgaggtgct ctacggttga aatccgtaca tcatgctcgt
    14761 tgaccagaca cgtacagagc atggtatcac gcacagattt ggcatcggcc gggaaatcta
    14821 ccggtggatt caagtcggtg cgacgataga tgaccccggt gttggccggc gcagggcgta
    14881 acgtcagggt gactttcttg ccggtatgta aaccgacacc cgtcgcctga acgatacgtt
    14941 taagtgtcct ttgtttgatc atcgtattat ctcgccaaat tacctatcca accgaagtgt
    15001 actatacatt cggcgggcca gtttagcaca aagagcctcg aaacccaaat tccagtcaat
    15061 tcttaatcag cttgcttacg caggaatgct gggatatcca gataatccgg ctctttcgca
    15121 gtttgcggcg cattgtcatt cacgacttta gcaaccggct tctgctcctg ggtcagcgga
    15181 gccatcccat gctgctggta gcgatccatc actggctgct gaacctgctt attggtcacc
    15241 agagtgattt caggacgttt gtccatgccg atacctgtcg caacaacggt tacgcgcagc
    15301 tcgtcattca tatccgggtc aagagaagta ccgataacca cagtcgcgtt gtcggaagca
    15361 aatgcacgga tggtgttacc taccgtttcg aactcatcca gacgcaggtc gaagcccgct
    15421 gtgatgttaa ccagcacgcc gcgtgcgcca gacaggtcga tatcttccag cagcggagaa
    15481 gagatagcca tttcagcagc ttcttccgca cggtcttcac cgctcgccac gccagaaccc
    15541 atcattgcgt agcccatttc agacattacg gtgcgtacgt ctgcaaagtc cacgttcatc
    15601 aaacccggac gagtaatcag ttcggcgatg ccttgcacag cgcctttgag tacatcgttc
    15661 gctgcgccaa acgcatccag cagggagata ccgcggccca gaactttcag cagtttgtcg
    15721 ttcgggatag tgatcagaga gtccacatgc ttggacagtt cagtgatccc ctgctccgcg
    15781 aatgccatac gcttcttgcc ttcaaagttg aagggcttag tgacgacagc aacggtcagg
    15841 atacccaaat cttttgccac ttcagcgacg actggtgctg cacctgtacc ggtaccacca
    15901 cccatacccg cagcaataaa gaccatgtct gcaccttcca gcgccgcacg cagtgcatcg
    15961 cgatcctcat ctgccgcatt gcggccaact tctggattag cgccagcgcc cagtcctttg
    16021 gtgataccgc taccgatttg aatcgtctgt ccaaccgctg ttttacgcag cgcttgtgca
    16081 tcggtattta ccgcgaagaa ttcaacacct tcaatgcgct cgcgcaccat gtgttcaaca
    16141 gcattaccgc cgccgccgcc gacgccgatg actttaatca ccgcgtcatt ggtaagttcc
    16201 attggttcaa acatagtttc tctccgattt gtgcctgtcg cctgaggccg taatcatcgt
    16261 cggcctcata aaaattaaaa ctcttttcgc agccaactat tgagtcgctt gatccacgag
    16321 ccaactgatg ctgtaacacg tttttctact tcagcttcac cgttaagatg tgactctttc
    16381 ccatagtgaa gcaatcccac cgccgtcgaa taatacggct cctgagcata atccgttaaa
    16441 ccggtaatgt tcagcggcgc gccgatacgc acttgcgtat gaaacacgcg ctgagcacag
    16501 gctgcaagac cttcgatctg cgctgcgcca ccggttaata caatgcctgc cgccaggtga
    16561 tgtttaaccc cttgttggcg aagcttttcc tgcaactgca atatctcttc gttgaccagg
    16621 ttgagcagct cggtatagcg cggctcgatc acctctgcca gggtctgacg ttgcagactc
    16681 cgcggcggac gaccgcctac gctcggcact tccacgctct catcttttcc aacgatggaa
    16741 cccagcgcac aaccgtggcg aactttaatc gcttcggcgt cgcttggcgg cgtgccaaag
    16801 gcgtaagcga tatcactggt cacgacattg ccagcataag gaattacctt agtgtggcgc
    16861 aatgccccac cggtataaac ggcgatatcc attgtaccac caccgatatc gacgacgcag
    16921 acacccagtt cacgttcatc ttccgtcaat accgaataac ttgatgccag accggcaaat
    16981 atcagttggt caactttcag cccacaacgt tcaaccgctt tgacgatgtt tttcgccata
    17041 tcgttgtggc atgtgatcag gtgcactttt gcctgcatcc gcacgcccga aagccctacc
    17101 ggattcttga tcccttcctg atagtcaatc gcatactctt gcgggatcac atgcagcaca
    17161 cgatgctcat cgcgtacacg caccgatttc gcggtatgga cgacgttttc cacatcttct
    17221 tgcgttactt cttcttcaga aataggcacc ataccaattt cattctggca gctgatgtgc
    17281 ttaccagaaa gcgccagata taccgaagag atctgacaat ctgccatcaa ttctgcctgg
    17341 tcaatggcac gttgtacgca cttgaccacg gattcgaggt cgttcacccc gcctttatcc
    17401 ataccacgcg acgggcagct gcccacgcca atgatattga ccataccgtc gggcagaact
    17461 tcccctacta aagcggcaac cttcgcggta ccaatctcca gtcctactac cagttttctg
    17521 tccgtcgcct tgatcattgt tgttctgcct gtgcctgatt ttgttgctga gtagattcct
    17581 ctggcggcaa gggcgcccag cctactgccg ctccagagtc ataacgcaaa tcaacgtagc
    17641 taatccgttt gccatcggtt tgcgcctgct gctgtagaac cggataaagt tctacaaagc
    17701 gagccaaacg tttcatcgta tcgccccggc caagattgag cttaatatcg ttattcagcg
    17761 tcaactgcca ggaacgccgc gcggtcatcg ccgcttcctt cagagtaaat ctgtcctttg
    17821 ccagcatctg ccccatttcg cgatagccct gcaacacttc attggcgctg ccttccgggc
    17881 catacagcat tggaagcacc tgcttgctgg tgcgatctgg cggcacgctg aaggtatttc
    17941 cttccgcgtc taccatgtgt tgatcattcc accgcgcaat cggcacatat tcaaccagat
    18001 gaatcttcaa ttcatcaggc cactgctttc tgacgctcac ctgcttaatc cacggcagac
    18061 gttgttctat ttgcgtctgg atgatgttga catcctgggt cataaaggta cccggctcac
    18121 ccaatgccag gatcgactgc cggatatcgt cattacgcgt gtaatggcgt tcaccggtca
    18181 acaccagctt tgagagcggc aggcgttgcg catcttccat ccagcccaac acgacccagc
    18241 cgctcaccaa cactgtcgtt aaaacggtca gcaggaaaag gatccccgcc agacgcgttc
    18301 cattattgcg gcgagaagaa acctcttctt cgctgtttcg cgtgttcaga gcagcctgcg
    18361 acatattagt ccgccagttc cagaattcgt actaccaact gcgagaagct catacctgcc
    18421 tgacgtgccg ccatcggcac caggctgtgg ctggtcatac ccggtgaggt attggcttcc
    18481 agcagataaa actgtccatc gctgtccagc ataacgtcaa tacgtcccca tcctttgcaa
    18541 cctaacgtcg tccatgcttt cagcactaat gcctgcaaat tggcctcttg tgacgcttcc
    18601 agacctgcgg ggcagaaata ctgtgtctca tcagagagat acttcgcctc ataatcatag
    18661 aaggttccgg acggttgaat acgtactgac ggtaaaattt cttcaccgag tatcgcaacc
    18721 gtgaactccg gcccacttag ccatttttca atcaatactt cttcatcgtg ctgaaatgcc
    18781 aatcttaatg catcttgtag agcattttct gctactactt ttgacattcc cacactggaa
    18841 ccttcgcggc tcggcttaac gataaccggc aaacccagag cagaaatttc tgctaactgc
    18901 ttatcactca ggcctttttc aaactctacg cgggttaacg ctacccacgg cgcgaccggt
    18961 aaaccggcac cttgccacag aagtttgctg cgtagtttat ccattgaaag cgcagatgcc
    19021 atcactccgc tgccggtata aggcaagccc atcagctcga gcatcccctg cagcgtacca
    19081 tcttcaccgc cgcggccgtg tagcgcgata aacactttct gaaagcccat cgacttcagt
    19141 tgcgtcacgt caacttcttt cgggtcgaca ggatatgcgt caataccgcc ttcacgcagc
    19201 ccggctaaca ccgctgcgcc agaattcaga gaaacttccc gctcagcgga agtcccgccc
    19261 aacaggaccg cgattttatc agtcatgttg ttcttcctcc ggagtttgcg gcttcagttt
    19321 gatttcagct aaagaacggg caatttttcc gatattacca gccccctgaa cgagaatcag
    19381 gtcgttaccg gttaataccg gtgccagcat ctcggctacc tgcgcagggt ccggcaccag
    19441 aatgggatct attttcccac gtccacgaat tgtgcgacac agcgaacggc tgtccgcacc
    19501 cggaattggc gcttcgccag ccggatacac ttccagcatc aacagggtat caacctgcgt
    19561 cagcacattg gcgaaatcat catacaggtc gcgtgtacgg gtaaaacggt gcggctgaaa
    19621 cagcattacc aggtttttat ccggccagcc tgcgcgcgcc gctttaatgg tggcgtccac
    19681 ttccgtcggg tggtggccgt agtcatcgac cagcattgcc gtaccgcttt taccattcac
    19741 tggctccagc gggaattcac cgaggaaatc aaaacggcga ccagtcccct ggaagctctc
    19801 cagcgcacgc aaaatagcct cgtcgtcaat gccctcttcc gtagcaaccg caaccgcagc
    19861 tgcggcgttc agcgcgttat gacggcctgg cgcattcagg gtgacgcgca tcggctcttt
    19921 gtcctggcgc agcagcgtaa agtgcccctg cgggccaatc tgctgataat cttctacacg
    19981 cacgtcggca tcttcgctga agccgtaagt tgtggtctga cgtcccactc gcggtaacaa
    20041 ttcgcggatc accggatcat caacacacat caccgcacga ccgtaaaacg gcaggttgtg
    20101 cagaaaatta ataaaagtct gttttaaatt ctcaaagtcg ccctggtagg tatccatgtg
    20161 gtcggcttcg atattggtga caatcgccac catcggttgc agatgcagga acgatgcatc
    20221 actctcatct gcttcggcaa tcaggtaccg accatgcccc aaacgcgcat gaacccccgc
    20281 cgcttttacc agcccgccgt taacgaaggt tgggtcgagc cccgcttctg cgtagatgct
    20341 ggagaccatc gcggtagttg tcgttttgcc gtgcgttcct gcaatggcga tgccatgacg
    20401 aaaacgcatt aactcagcca gcatttcggc acgacggatc accggaatac gcgcttcatg
    20461 agctgcgaca atttccgggt tatcggcaga aatggcgctg gaaacaacga ccacgctggc
    20521 atcacgtacg ttttccgggc gatggttgaa ataaatcgtc gctcccagat tcattaactg
    20581 ctgcgtgacc ggatttggcg ctaaatcgga accactgatc tgataacctt cattggccag
    20641 aacttcggca ataccgccca taccggcacc accgatgccg acaaaatgta tgtgccgaac
    20701 gcgacgcatt tcgggcacga tggaacgcag ttttgccaat tgttgtgtat tcattcttta
    20761 cgccattaac ttcttaaatt catacgatgc aaaaggcatc gctacaatta cgcccgggca
    20821 gcccgactca cttcatttgc cactcgctcg gtggcatccg gaatggatgc agcgcgggcg
    20881 cgttctgcca tggttaataa ggtttctcgc gaccacccgg ccagggtgtt ggcgacagca
    20941 tccacgctaa gctgtggctg ctcgataatt ttggctgcgc ccgctttttc cagcggtagc
    21001 gcattccagt attgctggcg gtctttatgt tgaaacggca caaacaacgc cggtagtcct
    21061 gccgcggcga tttcactcac cgttaaagca ccggagcggc aaacgacgac atccgcccac
    21121 gcatacgccg ccgccatatc atcaataaat tcagtcactt tatgctgcgg ttgccccgct
    21181 tcggcatacg cctgttcaac ggattgttgc gaacctttgc cgctctgatg ccagatagtg
    21241 actgaatcac ccagtttcgc agcaacctgc ggcattgtct ggttaagaat gcgtgcgccc
    21301 tgggaaccac ccactaccag cacacgaacc ggaccttcac gtccagccaa acgttgctgc
    21361 ggcaacggta gcgccaacac atcggtacgc accgggttac ccaccacttc cgcattgggg
    21421 aaagcacctg gaaacgcctg catcactttg gtggcaatct tcgccagcca tttattggtt
    21481 aagcccgcaa taccgttttg ttcatgaagt acaaccggaa tgcctaacga ccacgcggcc
    21541 agaccaccag gacctgatac gtagccgccc ataccgagca ccacgtcagg tttgtacgct
    21601 ttcataatcg cccgcgcctg acgccaggca ttgaagatac gcagcggcgc agctatcagt
    21661 gcttttatac cttttccacg cagaccagag atacgaatga aatcaatttc gatgccatgt
    21721 tttggcacta agtccgcttc catacggtcg gcagtcccca gccagcgaac ttgccaaccc
    21781 tgagccatta gatggtgcgc aaccgccagt cccgggaata catgtccacc ggttccgcct
    21841 gccatcacca ttaatcgctt tccttgagca ctcatcgtga acctcgtaca aacgcctgcg
    21901 ctttctccag acgcgtttca taatcaatac gcaacagcat catgatggct gtcgacataa
    21961 tcagtaagct cgaaccaccg taactgatca gcggcaatgt cagacctttg gtcggtaaca
    22021 tccccgccgc cgcgcctacg ttaaccagcg cctggaagct aaaccagatg ccaatagaac
    22081 aggcgagaaa accggaaaaa cggtggtcaa tttctaatgc tttacggcca atcgacatcg
    22141 cgcgaaaagc gacgaagaat accattaaaa gtgccagcac cacaccgaca taccccagtt
    22201 cttcgccgat aatggcgaaa ataaagtcag tgtgcgcttc cggcagatac tccagttttt
    22261 gtacagagtt acctaaacct tgtccccaca gttcgccgcg accaaacgcc atcagcgatt
    22321 gcgttaactg atagccgctg ccaaagggat cttcccacgg gttccagaat gcggtaacac
    22381 ggcggatacg gtacggttcg gcgagtatca gcaacacaac cgctgaaatg cccataccga
    22441 taatggcaat gaactgccac aatttcgctc ccgccaggaa caacatcgcc agcgtagtca
    22501 caaacaacac caccaccgta ccaaggtctg gctgtgccag cagtaacact gccaacacca
    22561 gaatcacgcc catcggtttc aggaagccgc gcaggttatt acgtacttcg tcacctttac
    22621 gcaccagata gttggcgata tagcaaaaca gcgacagttt tgtcagctca gcaggctgga
    22681 tacgcagcaa accgagatcg atccaacgcg atgccccttt aaccgagctc cccactacca
    22741 gcacgatcat cagcaggatg atagatccga gcagcatcgt ggcactgtag cgttgccaga
    22801 actccatcgg cagacgcagc gtaatgatcg ccagaataaa cgccaaaatc agatagacac
    22861 catcacgctt cgcgaagaag aacggatcgt tggttaagcg ttgccctatg ggcattgacg
    22921 ccgaggtcac catgataaag ccaatcgccg cgaggccgaa ggtcagccac agtaaggtgc
    22981 gatcgtacat gatcaggctg tcggtatctt tttcccgcga gcccatcacc cagcccttta
    23041 gcgccgtgga gatccagacc aggatactga atcctggcag gcgcggcatt ttcaggcgag
    23101 ggagagataa acgcatcaac ctaactcctt cgccagacgg gcaaactcat tgcctcgttg
    23161 ttcaaagttc ttgaactgat caaggctggc acaggccggg gagagtagaa ccatatcgcc
    23221 cggctgaaca cgcggagcca gcaagcgcat cgcctgttcc atagtttcgg tttgttctgc
    23281 cacttccggg cgtagcgccg ccagctgcac gccgtcacga ccgaaacaat acagacgtac
    23341 gttatcgcca ttcaggtaac gcgccagtgg gctaaagtcc gccgatttac catcgccgcc
    23401 cagcaacaaa tgcagcgtgc cgtctacgtg caggccattc agcgccgctt ccgtactgcc
    23461 gacgttggtc gctttcgaat cgttaatcca acgtacgccg ttatgctcca gcacaacttc
    23521 aaagcgatgc ggcagaccag tgaatgtggt taacgctttc aggctgctgg cacgcggtaa
    23581 ccctgcggca tctgccagcg ccagcgccgc cagcgcattg gtgtagttat gctgcccgga
    23641 aagtttcatt tctttcacgt tcagcacttt ctcgcctttc acccgcagcc aggtttcgcc
    23701 ctgctgatga ttcaggtgat agtcacccat gttgacgcca aagctgacgc agcgttcatc
    23761 cgcaccgcga atcggcattg ttaaggcatc atcagcattg acgacacaaa ctttcgcatt
    23821 ttcgtaaata cgcaattttg ccgcacggta ttgctgtaaa ccaaacggat agcgatccat
    23881 atgatcttca gtcacgttca gaatggtcgc tgctaccgcc tgtaagctgg aggtggtttc
    23941 cagctggaag ctcgacagtt ccagtacgta cagttcgcac tcatcatcca gcagcatcaa
    24001 cgcgggcagg ccaatattgc cacccacacc aacgttaacc cccgccgctt tcgccatttc
    24061 acccactagc gtggtgaccg tgcttttgcc gttagaaccg gtaatcgcca caatcggtgc
    24121 ttgtgcttcg cgacagaaca gctcgatatc gccaacgatt tcgattccgg catcagcggc
    24181 agcgcttaag gatggatgcg ccagtgcaat accgggactg gcgacaatca gatctgccgc
    24241 catcagccat tcatcattca gactgcccgt gtggcgttct acggcttcgg gtaatttatc
    24301 caggccaggc ggtgtcatac gcgtatccat aacgcgcggc gtcacaccgc gagcgaggaa
    24361 aaagtccacg caggaaagcc cggtgaggcc caggccgata atgacaacat ttttaccctg
    24421 gtaatcagcc atgattaacg taccttcagc gttgccagac caatcaggac cagcatcagc
    24481 gaaataatcc agaaacgcac aatgacgcgc ggttccggcc agcctttcag ttcatagtgg
    24541 tgatgaatcg gtgccatgcg gaaaatacgt tgtccgcgca gtttaaagga gccgacctgc
    24601 aggatgacgg aaagcgtttc taccacgaac acgcccccca taatcaccag caggaattcc
    24661 tgacgcagca gtacggcgat aatgcctaac gcaccaccta acgccagcga acctacatcg
    24721 cccataaaga cctgcgccgg ataggtgtta aaccacagga agcccagccc tgccccgact
    24781 atcgcggtac agacaataac cagttccccg gcgtgtcgca gatatggtat gtgtaaatag
    24841 ctggcaaagt tcatgttgcc ggtcgcccac gccaccagcg caaaaccacc ggcgacaaat
    24901 acggtcggca taattgccag accgtcaaga ccatcggtca ggtttaccgc gttgccagta
    24961 cccacaatga cgaagtaagc cagcagaatg tagaacagcc ccagttgcgg catcacatct
    25021 ttaaagaatg ggaccaccag ctgcgttgcg ggcgtgtctt tgccaacaag gtacagcgcg
    25081 aaggcgacac ccagcgcaat gaccgacatc cagaaatact tccaacgagc gatcaaccct
    25141 ttggtgtctt tacgcactac tttgcgataa tcatcaacaa agccaataac accgtaacct
    25201 accagcacca ccaacacgca ccagacgtac ggattggacg ggtaagccca cagcagtacg
    25261 gagatcacaa tcgccgtcag gatcataatc ccgcccatgg tcggcgtacc gcgcttgctg
    25321 aagtgtgatt caggaccgtc gttacgcacc acctgaccaa aggaaagttt ttgcaaatga
    25381 gcaatcatac gcgggcccat ccacaatgag atgaacagcg cggtcagcag gctgacgatg
    25441 gcgcgaaacg tcagatagga aaagacgtta aagccggaat aatatttgac caaatgttcg
    25501 gccagccaaa ctaacatgtc ccattctcct gtaaagcgcg tactacctct tccatggcgg
    25561 cactacgtga acccttaact aaaatcgtaa ttacctgttg ctcagcaatc agtgatttaa
    25621 gacgcgtaat taacgcagtt ttctcagcaa aatgttcgcc aacgccgctg gcggtgctga
    25681 tagcatggct ttgtttaccc acgcttaaca cgcggtcaat accagcagct tttgccgcct
    25741 cgcccacctg tacatggcag gcttcgcttt cagcgcccag ttccgccata tcgcccacca
    25801 ccagcacgcg gtagcccggc atttcagcca gtacctgaac agcggcggtc attgaaccga
    25861 cattggcgtt gtaggagtcg tcgagcagca actggttttc tgccagttga atggggaaca
    25921 aacggcctgg aacagctttc agatttgcca gccccgcttt gatagcatca agcgttgcgc
    25981 ccacggacat ggagagcgca gcggctgcca gcgcattcgc aatattgtga cgccccggca
    26041 acggcagcag aacatccacg ctaccggttg gggtttgcag ggtaaattcc gtaccgtgcg
    26101 aagtcacatg gatattggtg gcggtgaaat cgctgttggc ggcattaggt gagaaacgcc
    26161 acactttgcg tgagccaatt acgctctgcc agttcagcca gtcgttgttg tcggcgttca
    26221 taatggcgat accgttttcc ggcaggccgc taaagatttc acctttcgct ttcgcgacac
    26281 ccgcaagcga gccaaaacct tccagatgcg ccgctgccag gttgttgacc agcgcagctt
    26341 ccgggcgagt cagactcaca gtccagacaa tttcgccctg atggttcgcg ccaagttcaa
    26401 taactgcgta atcgtattcc ggcgttaagc gcaacagcgt catcggtaca ccgatgtcgt
    26461 tgttgagatt gcctgccgta taaagcgtgt tgccgcactg gcttaaaatc gccgccgtca
    26521 tctctttaac ggaggttttg ccggaggaac ctgtcagagc aaccacgcgc gccggaactt
    26581 gctggcgaac ccatgcagcc agttcaccaa acgccagacg cgtatccttg acgattaact
    26641 gcggcaggtc gatatccagc ggacggctaa ccagtagtgc gcctgcgccg ccagctttcg
    26701 cctggtcggc aaaatcatga gcatcgaaac gttcgccttt cagggcaaca aacaggcagc
    26761 ccggcgtcag ttttcgggta tcagtggtta cagcatcaag ggtgatatct gcaccttgca
    26821 gttcaccgtt gagaatgtcg gtaagttggc taagggttac gctaatcatg caatcacccc
    26881 cagcagacgc gccaccgtga cgcgatcgga gtagtccaga cgctgattgc caacaatctg
    26941 gtaatcttca tggcctttgc ccgcgaccag caccacatcg ttctctttag cctgcataac
    27001 ggcgcaagtc accgcttcag cacggtcttc catcactttg gcatgtccgg catctaacat
    27061 tcccgccaga atatcgttga tgatggcacg cggctcttcg gtacgcggat tatcgtccgt
    27121 caccaccgcc acatcagcaa actcttcggc aattgcgccc atcagtggac gcttaccttt
    27181 atcgcgatcg ccaccacagc caaagacaca ccacagcttg cccgcacagt gcagacgcgc
    27241 cgcctgtaag gctttttcca gtgcatccgg cgtatgcgcg taatccacca ccaccgtcgg
    27301 tttgcctggc gcagtgaaca cttccatacg tccgcaaacc ggttgcagac gagcagcggt
    27361 tttcagcaga tcagccagtg gatagcccag cgccagcagc gtcgccagcg cgagcagcag
    27421 gttgctgacg ttaaaagcgc ccatcagatg gctttcaatt tcgccatcgc cccaacttga
    27481 gctaaagcga atagtcgcac cgctgtcgtg atagttcact tcggtcgctt tcaaccagcg
    27541 tccgtgacag ttcggattga tatgatcttc cattgatacc gcaactgcat ccggcagttt
    27601 tgccagccag cggcggccta cttcatcgtc ggcattaata atcgcctgac cgcaatgatg
    27661 ttcagaataa agcagccatt tcgcagcttc gtagtgttcc atatcaccat gataatcaag
    27721 gtgatcgcgg cttaagttag taaagaccga cgccgcaaat ttcaatgccg ccacacggtg
    27781 ctgtaccagc ccgtgggagg aaacttccat tgcgcaaaaa gtcgcgtcct gatccaccag
    27841 ccccgccagc tcatgctgaa catcaactgc cgaaccggtg gtattttctg tcgggatcac
    27901 tttccccagc agaccgttac caacggtgcc cattaccgcg ctggtttcgc caagcagttg
    27961 gctccactgc gccagcagct gggtcgtcgt ggttttgccg ttggtgcccg ttacgcccac
    28021 gagacgtaaa ttgtcagagg gttcatggta aaagcggccc gccagtgcag ataaacgctc
    28081 gttgagctgg ctgagataga tgaccggtac gccgtgcatt tcacggattt caccgtcggt
    28141 cgcctcacct ttcgcctctg caataatggc agccacacct tgcgctatcg cctgcgggat
    28201 atatcgacgc ccgtccgcct gatgacctac tacagctaca aagagatcgc ccgccgcagc
    28261 cacacggctg tcgagtgtca tctctcgcag tgctcgcgaa ggtgcgtctg gcacccacgg
    28321 agcaagaagg tcgcgcaaat tacgatctgc cacctgtccc ctcgccttga ttaatcacaa
    28381 attcattttt atcgcccgtt gtcagcgcat ccggctcgat gttcatggtt cgcaatacgc
    28441 cgcccatgat ggcaccaaag accggcgcgg aaacggcgcc gccgtagtat ttacccgcct
    28501 gcggatcgtt gataacaaca accagcgcga agcgcggctg actcgcaggc gcaacacctg
    28561 cggtgtaagc aatatattta ttgatgtagc gaccgtccgg cccgaccttt ttcgcggtac
    28621 cggttttaat ggcaatacga tagcctttaa tcgccgcctt cacgccgccg ccacccggta
    28681 gcgccacgct ttccatcata tgcaccacag tgcggacaat agattccggg aagacacgtt
    28741 caccgggaac cggggggtca actttggtaa tcgacagtgg gcgataaatg ccgtagctgc
    28801 cgatagttgc gtagactcgc gctaactgta acggagttac cattagcccg tagccgaaag
    28861 agaaggtggc cctctctatg tcagaccacc gttgtttttg aggatataag ccactgcgtt
    28921 ctccgaccaa ccccaaattg gtcgcttttc ccagtccaaa acgtgagtaa gtatctacta
    28981 acgctgagga cggcatcgct aacgccagct tggaaacacc gacgttactc gacttctgta
    29041 atactccggt cagggttaat tcgctgtagc gtgccacgtc tttgatttcg tggccgttaa
    29101 ttcgataagg aatggtattg agtaccgagt tttcccgcac cacgccacgt tgcaacgcgg
    29161 tcatcaccac catcggttta accgttgaac ccggttcaaa cacgtcggtg atggtacggt
    29221 tacgcatcgc ctctttcggc gtgccgctca gattgttagg gttgtatgac gggctgttag
    29281 ccatcgccag cacttcaccg gtgttgacat ccaccagcac ggcgctaccg gactcagcct
    29341 tgttaaaggc caccgcgttg ttcagttcgc gataaaccag cgcctgcagg cgttcatcaa
    29401 tactcagcgc caggttgtgc gcagcctggc tgtcagtaga agaaatatct tcaattacgc
    29461 gaccatagcg gtctttacgc acaatgcgct cacccggctg cccggtaagc catttatcga
    29521 aactcttctc aacgccctca ataccttgac tatcgacgtt agtaaagccg atgaggtgag
    29581 cagtcacttc gccggatgga taataacggc gagattcttc acgcagatga attcccggca
    29641 gtttcagttt tttgatgtag tccgccatgt cagggtttac ctgacgcgcc agataaataa
    29701 agcgcccttt cgggttggcg ttaatgcggg cagaaagctg atccagcgga atattgagcg
    29761 cgttagccag cgccttccag cggtcaccga cgctgatacc gccagcgtca tgcacttctt
    29821 tcgggtcagc ccaaatcgct tttaccggca cgctcaccgc taacgggcga ccagaacggt
    29881 cagtaatcat gccgcgggag gtggaaactt gctgaacgcg aagagaacgc atgtcgccct
    29941 ctttcaccag catatccggg gagataactt gtaaccacgc tacgcgtccg agcagaaaag
    30001 ccagcgccag gagaatacag ccgcataaca acgcaaaacg ccaactgata aagttggcat
    30061 gttcttcctg acgttttggt ttctgcgttt tcgccgctgc tttcatgcgt cgcgtttatc
    30121 cttatttttg cactacgata ttttcttgtg acggatcaac atgctgcatt tgcagctttt
    30181 ccgtggcgat cctttccacc cggctatggt cgccgagcgc attctcttca aggatcaggt
    30241 tgcgccattc aatgtctaaa gcatctcgct ccagcaccag ttgttcgcgc tgagcggtca
    30301 gtaaacgggt atggtgcgcc gtggttacca cggtcaccgc cgtcaaaata atgcaaatga
    30361 acaggcagag tggcagcttc ccaaatcgca aaagatcgtc accgataaca ccaggcaatg
    30421 catggcgctc gtggcttccc atcgatcctt taactttgct tagagcttct gtcactctgc
    30481 tgatcatgcg ttcgtcctct ctgcaatacg cagaactgaa ctacgggcac gaggattctc
    30541 agccacctct tcttcgcccg gcattaattt gcctagtgct cgcagctgac ggccacccag
    30601 ttttttgagc tgctcttcag tcatcggtaa ccctgccgga acttgcggac cgcggctgtt
    30661 ttcacgcata aaacgtttca caatacggtc ttccagcgag tggaagctga tgatcgaaag
    30721 ccgcccaccc ggggccagca cgttgagcga gctttttagc gcctgctcta tctcctccag
    30781 ttcactgttt acccaaatgc gcaccgcctg gaaggtacgg gtcgcgggat gtttaaactt
    30841 atctttcacc ggcgttgcag cagccacgac ttccgccagt tctttggtgc gggtcatcgg
    30901 ctgttcgcgg ttacgctcga caatggctcg ggcaatgcgt ttggcaaaac gctcttcacc
    30961 ataggttttc aatacccagg cgatatcggc ttcttctgcg gtttgtagcc attcagcggc
    31021 tgactgccca cgggttgggt ccatacgcat gtccagcgga ccatcgcgca taaaggaaaa
    31081 gccgcgttca gcatcatcaa gttgcggtga agagacacca agatcgagga gaatgccgtc
    31141 gatcttgccg ataagatcgc gctcggcaac gtattcgccc agcgcggaaa aaggtccgtg
    31201 gatgatggag aagcgcggat catcaatagt cttcgcaacg gcgatagcct gcgggtcgcg
    31261 atcgatcgcc agcaaacgcc cctcttcgcc aagctgcgag aggatcagac gtgagtgacc
    31321 accgcgacca aaagtcccat caatgtagat gccatcagga cggatattga ggccattaac
    31381 ggcttcatcc agcagcaccg tagtatgttt atagttttcc atcattttat agagacaagt
    31441 cctgcagtcg ctccgataag tctccggtag ccaactgctc tgcgtcgata tcttccttga
    31501 cctgttgatg ccaggttgtt tcatcccaca gctcaaactt gttgaactgt ccaaccagca
    31561 tcacttcttt tgtcagcccg gcatgttgcc gcagtactgg cgcgattaac aatcgacctg
    31621 cgccatccat ctgacattcg ctggcatgac ctaacagtag gcgctgcaca cggcgctcaa
    31681 ccgggttcat gctcgacaga cgcgataatt tttgctcgat aatttcccat tcaggcaggg
    31741 ggtaaagcag caggcacggg tgatgaatgt caatggtgca aaccatttga ccggcagcgt
    31801 tctcaagcag ctgttcccga taacgggtag gcactgataa gcgccctttg ctgtcgagat
    31861 tgactaacgt tgctccccgg aacatgccag cctcacccct tattcccact ttgccccaca
    31921 atttcccact gaaaggagtt tacggagcgg aggaaaagct tgtcaagcca gtcacaaggc
    31981 ataccgaggt aaaaaacccg atgtttacgg ctaatatcag cgttgtttat tttacgtcca
    32041 acgtgcgaag caaattaacg ttatgaatag ctgtaagaaa aaaaccctca tcgtcatcac
    32101 cgttttaaaa tgactcacta ttttccacag caaatataaa gtgtcagttt gcgacgcgag
    32161 cggcatttta ggacatatct tccccggtta acagtccctg ttgcgtctgt gtagcgcttg
    32221 atgccagcgg gtttgcgcaa aagcaaagaa aagtgcttgt taaatcggca attcatctct
    32281 gcatgtaaca aaataatgca aaaacgcgct gtcgatatca cttaattatt gtgaatttca
    32341 gctggtgagt taattaactg gcgggtttaa aagcatttat caataatagt caggaatagt
    32401 cttatttact ttaaggatat tgatgtccag tcccgtactc tacgcgccag agtgaaattc
    32461 acctggcgcg tatttttgtt cgcagcttag ctacggctga gcacaccgcg gcgatagaga
    32521 ttacgtttaa tgcgcgttaa accaggtttt ggcttacgcg gttcgtccag gcttgccagg
    32581 acaatctcca gcacacgctc tgcgacatcg cggtgacgtt gagccactgc cagcaccggg
    32641 cactgtaaga agtcgagcag ttcgttatcg ccaaaggtgg caattgccag gtcagaaggc
    32701 agtttaccat cgcgacgtag cgtgacatcc atcacccctt gcaacaacgc aaacgacgtt
    32761 gtgaacagcg cctgcggcat cggatgcgtt tccagccatt tttcgaataa ctgggcagcc
    32821 gcctcccgct catagctgtt ggcatacagg aaatgcactt cgcgcggatc atctttccag
    32881 gcagtacgga aaccttgttc acgcaggaag ctgacagaaa gctccggcag cgcgccaaga
    32941 taaagcaccg tctcggcggg aaacttacgt aactcttccg ccagcatttc ggcatcatcc
    33001 tgatcggcac caaccacgct ggtaaagtgt tcacgatcga gggcgcggtc cagcgcgaca
    33061 atcgggaacg ggtcgttagc ccagcgttga taaaaaggat gctcaggagg caacgacgtc
    33121 gaaacaataa tggcatcgac ctgacgctgt aaaaggtgct caatgcagcg catttcgttg
    33181 tctggctgat cttctgagca ggcaatcagc agttgataac cccgttgccg cgcctggcgt
    33241 tcaagatagt tagcgatgcg ggtatagctg gtgttctcca gatcggggat cacaagacca
    33301 atagaacgtg tgcgtccagc acgaagccca gctgccacgg cgttcgggtg gtaattgtgc
    33361 tcacgcacca cagccatgac tttttcaacg gttttgtcac tcacacggta ttgcttcgct
    33421 ttgccgttaa taacatagct tgcagtggtc cgcgacactc ccgccagccg agcgatttca
    33481 tccagtttca caattgcccc ttgcgtaaaa tgtaaaaacc atagccattg tactggtatg
    33541 ggttaaattc attaacatct aagcgcagaa aattaactgc ggcaactgct tttattccag
    33601 gttcccactg atttcgcaaa aaaagcccaa cgtgacatgt tgggctgtaa attgcgcatt
    33661 gagatcattc tcaacgcatt attttatcgc cgcgcgaaag tccgaccaca ccagagcgag
    33721 caacttccac aattttcgcc acatcgcgaa tcgatgctaa aaatgcatca agcttatcgc
    33781 tggtgcctgc taattgaacg gtataaagcg agggtgtgac atcaataatt tgcccacgga
    33841 atatttccgt attacgttta acttcgtcac gcccgtaacc gctggcctga attttcacca
    33901 gcatgatttc cctctcaaca tgcgcgccct gccccaactc actcacgcgc aagacatcga
    33961 ccagcttgta taattgcttt tcgatctgct caagcacttt ttcatcgccc acggtctgga
    34021 tggtcatacg cgataatgtc ggatcgtcgg ttggcgcaac ggtcaggctt tcaatgttgt
    34081 agccacgctg ggaaaaaagg ccaatcacgc gggataacgc gcctgattca ttttcgagta
    34141 agactgataa tatccggcgc ataatcaggt tctctccgtt ttgcttaacc acatttcatc
    34201 cattccgccc ccgcgaatct gcatcgggta gacgtgctca ctgccatcga cggtgacatc
    34261 aacaaatacc aggcgattat tacgcacctg ttcaagcgcc tcgctaagtt tgctttccag
    34321 ctcttgcgga tgagagatct ggatcccgac gtgaccatag gcttccgcca gacggacgaa
    34381 gtctggtaaa gactgcatat aagattgtga atgacgaccg gaatagatca tgtcctgcca
    34441 ctgcttcacc atccccaaat agcggttatt aagattaacc accagcaccg gcagttcata
    34501 ttgcaacgct gtagacagtt cctgaatgtt catctgaata ctgccgtcgc cagtgacgca
    34561 aaccacggtt tcttctggca gcgccatttt gacgcccagt gccgcaggta aaccaaagcc
    34621 catagtgccg aggccaccgg agttgatcca acgacggggt ttgtcgaatg gataataaag
    34681 cgcagcaaac atctggtgct gcccgacatc ggacgtaacg taagcgcccc ccttcgtcaa
    34741 ccgccaaaga gtctcgatca ccgcctgcgg tttaatcttt tcactgtgag tgtcatattt
    34801 caggcactga cgagcgcgcc actgttcaat ctgctgccac cagtcgcgga tctcatccag
    34861 cggttgatgg gcggattctt gcgacaagag ttcaagcatt tgttcgagga cctggcgagc
    34921 atcccccaca atcggaatat ccgcagtcac ggttttagaa atggaagtag gatcaatatc
    34981 gatatgcaga acagtggcat ttgggcagta ctttgccaga ttgttagtgg tgcgatcgtc
    35041 aaagcggaca ccgacagcaa aaatcacatc cgcgttatgc atcgtcatat tggcttcgta
    35101 ggtaccgtgc attcccagca tgcccagtgc ctgacgatgc gttgccggaa acgctcccag
    35161 ccccatcaat gaggaaacaa cgggcagatt taacgcctcc accgtttctt tcagctgttg
    35221 atggcagccc gccgtgattg ccccaccgcc tacgtagaca accggttttt ttgccgccac
    35281 cagcgtttgc agagcgcgct taatttgccc tttatgtccg gtagtagtgg gattgtaaga
    35341 acgcatactg accgactccg gccagacata gggtaatttg ttcgccggat taagaatatc
    35401 tttcggtaaa tcaacgacca ctggtcctgg gcgaccactt gccgccagcc agaaagcctt
    35461 tttcagcacc tgcggaatgt cttccgtttg cttaaccaga aaactgtgtt taaccaccgg
    35521 tcgcgaaatc cccaccatgt cgcactcctg aaaggcatcg taacctatca acgaggtcgc
    35581 tacctgcccg gaaaggacaa ctaatggaat ggaatccata taagcggtgg cgatgccggt
    35641 aatcgcattg gtcgcccctg gacccgacgt taccagcacg acgccgactt ccccggtcgc
    35701 gcgcgccaga ccatcggcca tatgcactgc cgcctgctca tgacgaacta aaacatgacc
    35761 gataccaccc acggtatgca atgcatcgta aatatcaagg actgcgcctc cgggataacc
    35821 gaatacttgt ttaacgccct gatcgataag cgatcggacg accatctcgg ctccagacaa
    35881 catctccatg gcctgcctca ctgtttgacg gaaaaaatgt gtaaaacaca ttaaccgtcc
    35941 gatcttgctt aagcaattgg cagccagaga cataaacgcc gggtaataag aataaacagg
    36001 aggaaaggtg aaaagaaacg ataaaatcct ccattacgaa ggataaaaat gataataaat
    36061 cggctaaatc ccaccactta cagcaatttc tctatttttt taattaatcc taaaatagag
    36121 agcccacaca ttcagaataa aattctacac tcaataaaaa atgcagaata aaccagacat
    36181 tcatgtctga cctattctgc attcagttag cgtttgcaaa ttgagactaa ttgctcttcc
    36241 atccactgat ggcctttatc acgcccggca gcttcatgcc aggagagata acaggttctg
    36301 ctatttagtt ttaacggcag cggtaatacc tgtaattcta aggattcagc gaactcttca
    36361 gccagccaac gcggcgcaat agcgaccaaa tgcgtttgcg acaccacgct aagtacgctc
    36421 atcattgcca tgccctgata cgcgatactg gcttgcttat ctaccgtgtc ataccaaggt
    36481 tgactaaatg acgcgaaacg atcgagcgaa accgccgcat gttgttcgtt ataaacatca
    36541 tgtttcagta acgggccctt aattgttggg tgatttttgc tggctaccag caccatttca
    36601 tctttaaata atggcacgct ggtaaattca ggacgatgga agtcttcata actaatcaca
    36661 aactccgttt cctgataacg cagctgatgt tcagtgttct gatttaatga agacttgaac
    36721 ataacatgta tatttggcgc aatctgctca atgtgattat aaatctgcga ggtcagaata
    36781 ctgtctaacg ggctgcaaac acaaagatga aatacacgtt cactgctcgc gggttcaaaa
    36841 cctgaaccag gcaattcatt ttgtactagt tgcaatgcct gacgaactga accaaaaagt
    36901 tgaaatgcgc gagcagtcgg ttgaatacca cggccataac gaacaaaaag ctcgtcatta
    36961 aacatcacct tcaggcgtgc aacagcgtta ctgaccgcag gttgcgacat tcccagaaca
    37021 tgagcagcac gggtaatgtt ttgctcctgc atcacggcat cgaaaacggt taataagttg
    37081 agatcgacca tgcgtagctg tggcttgctg aactccgccg tctctggatg atctgtttgt
    37141 acctctggca tacttaactc cactgtcaca cttaactccc tttcccttat tggaatgcag
    37201 agaaataatc ctcgagaata tgatttatca tgcatataaa ataagaaaaa ggaaaatgag
    37261 taaaattagg aaaacataaa gatacgggtt cttataaaaa ca
//
LOCUS       DBFIFI010000024        36942 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.024, whole genome shotgun sequence.
ACCESSION   DBFIFI010000024 DBFIFI010000000
VERSION     DBFIFI010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36942
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.024"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            166..1749
                     /gene="yegH"
                     /locus_tag="N7B39_001200"
     CDS             166..1749
                     /gene="yegH"
                     /locus_tag="N7B39_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HGU2421216.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            1824..2162
                     /locus_tag="N7B39_001201"
     CDS             1824..2162
                     /locus_tag="N7B39_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGU2421217.1"
                     /translation="MRKKLAFLDTSLDDLRAFPESSRQEIGYQLDRIQQGLNPYDWKP
                     FSTIGPGVREIRTRDADGIYRVMYVAKFEEAVYVLHCFQKKTQTTSQSDIDLAKRRYK
                     ELVQERKNEN"
     gene            2152..2442
                     /locus_tag="N7B39_001202"
     CDS             2152..2442
                     /locus_tag="N7B39_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU2421218.1"
                     /translation="MKIETFDSVWDAVSDTPEQAENMRIRAELVTIINNWIEQQGFSQ
                     AQAASALGVTQPRISELARGKIQIFSIDKLITMMAHAGLHIQRIEVQYPHAA"
     gene            complement(2495..4348)
                     /gene="asmA"
                     /locus_tag="N7B39_001203"
     CDS             complement(2495..4348)
                     /gene="asmA"
                     /locus_tag="N7B39_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HGU2421219.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESRKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMADTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLAAQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(4370..4951)
                     /gene="dcd"
                     /locus_tag="N7B39_001204"
     CDS             complement(4370..4951)
                     /gene="dcd"
                     /locus_tag="N7B39_001204"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HGU2421220.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDESEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(5043..5684)
                     /gene="udk"
                     /locus_tag="N7B39_001205"
     CDS             complement(5043..5684)
                     /gene="udk"
                     /locus_tag="N7B39_001205"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HGU2421221.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            6002..9320
                     /gene="dgcE"
                     /locus_tag="N7B39_001206"
                     /pseudo
     CDS             6002..9320
                     /gene="dgcE"
                     /locus_tag="N7B39_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(9421..10269)
                     /gene="alkA"
                     /locus_tag="N7B39_001207"
     CDS             complement(9421..10269)
                     /gene="alkA"
                     /locus_tag="N7B39_001207"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HGU2421222.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADDYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSADLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            10403..11755
                     /gene="yegD"
                     /locus_tag="N7B39_001208"
     CDS             10403..11755
                     /gene="yegD"
                     /locus_tag="N7B39_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HGU2421223.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARIQEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(11768..13708)
                     /gene="yegI"
                     /locus_tag="N7B39_001209"
     CDS             complement(11768..13708)
                     /gene="yegI"
                     /locus_tag="N7B39_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HGU2421224.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQSYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLPSFVGFERTANHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDIAHFRYAYASDNQRRGLKPPPRSIPLSMLPGDVEAMFQQ
                     AFTESGVATARPTAKAWVAALDLLRQQLKKCTVSAMHVYPGHLTDCPWCALDNQGVIY
                     FIDLGEEVITTSGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILIEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKVYKAEIQQRREAFNRA
                     KMDYDHLVSQIQQLGGLEGFIAKRAMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDAASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAVTPAERQAVMAKMAAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(13705..14466)
                     /gene="pphC"
                     /locus_tag="N7B39_001210"
     CDS             complement(13705..14466)
                     /gene="pphC"
                     /locus_tag="N7B39_001210"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HGU2421225.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAMLAINEAMAYVSQKVQHGEFGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDVVSRLETFTSTERAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLAAATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLTLALWLP"
     gene            complement(14463..15122)
                     /gene="yegL"
                     /locus_tag="N7B39_001211"
     CDS             complement(14463..15122)
                     /gene="yegL"
                     /locus_tag="N7B39_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HGU2421226.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMNGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADIKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(15343..15402)
                     /gene="ibsA"
                     /locus_tag="N7B39_001212"
     CDS             complement(15343..15402)
                     /gene="ibsA"
                     /locus_tag="N7B39_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HGU2421227.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(15674..15730)
                     /locus_tag="N7B39_001213"
     CDS             complement(15674..15730)
                     /locus_tag="N7B39_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HGU2421228.1"
                     /translation="MMKWIIIVVLLVISFPAY"
     gene            complement(16002..16058)
                     /locus_tag="N7B39_001214"
     CDS             complement(16002..16058)
                     /locus_tag="N7B39_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HGU2421229.1"
                     /translation="MMKILIIVVLLVISYPAY"
     gene            16338..17585
                     /gene="mdtA"
                     /locus_tag="N7B39_001215"
     CDS             16338..17585
                     /gene="mdtA"
                     /locus_tag="N7B39_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HGU2421230.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLTNARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLLFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            17585..20707
                     /gene="mdtB"
                     /locus_tag="N7B39_001216"
     CDS             17585..20707
                     /gene="mdtB"
                     /locus_tag="N7B39_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HGU2421231.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAITLVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQISDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            20708..23785
                     /gene="mdtC"
                     /locus_tag="N7B39_001217"
     CDS             20708..23785
                     /gene="mdtC"
                     /locus_tag="N7B39_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HGU2421232.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGMVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKSKQTVTE"
     gene            23786..25201
                     /gene="mdtD"
                     /locus_tag="N7B39_001218"
     CDS             23786..25201
                     /gene="mdtD"
                     /locus_tag="N7B39_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2421233.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAITGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSSTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            25198..26601
                     /gene="baeS"
                     /locus_tag="N7B39_001219"
     CDS             25198..26601
                     /gene="baeS"
                     /locus_tag="N7B39_001219"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HGU2421234.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            26598..27320
                     /gene="baeR"
                     /locus_tag="N7B39_001220"
     CDS             26598..27320
                     /gene="baeR"
                     /locus_tag="N7B39_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HGU2421235.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLSYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            27511..27843
                     /gene="yegP"
                     /locus_tag="N7B39_001221"
     CDS             27511..27843
                     /gene="yegP"
                     /locus_tag="N7B39_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HGU2421236.1"
                     /translation="MAGWFELSKSSDSQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            27991..29352
                     /gene="yegQ"
                     /locus_tag="N7B39_001222"
     CDS             27991..29352
                     /gene="yegQ"
                     /locus_tag="N7B39_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HGU2421237.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(29626..29844)
                     /gene="ogrK"
                     /locus_tag="N7B39_001223"
     CDS             complement(29626..29844)
                     /gene="ogrK"
                     /locus_tag="N7B39_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage transcriptional regulator OgrK"
                     /protein_id="HGU2421238.1"
                     /translation="MFHCPLCQHAAHARTSRYITDTTKERYHQCQNVNCSATFITYES
                     VQRYIVKPGEVHAVRPHPLPSGQQIMWM"
     gene            complement(29926..31089)
                     /locus_tag="N7B39_001224"
     CDS             complement(29926..31089)
                     /locus_tag="N7B39_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016157990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HGU2421239.1"
                     /translation="MLDALTFDAGSTLTPDYMLMLDSRDITGNISDRLMSMTLTDNRG
                     FEADQLDIELNDADGQVGLPIRGAVLTVYIGWKGFALVCKGKFTVDEVEHRGAPDVVT
                     IRARSADFRGTLNSRREDSWHDTTLGAIVEAIASRNRLEASVAPSLAGIKIPHIDQSQ
                     ESDAKFLTRLAERNGGEVSVKMGKLLFLKAGQGVTASGKKIPQVTITRSDGDRHHFAI
                     ADRGAYTGVTAKWLHTKDPKPQKQKVKLKRKKKEKHLRALEHPKAKPVRQKKAPKVPE
                     AREGEYMAGEADNVFALTTVYATKAQAMRAAQAKWDKLQRGVAEFSIILATGRADIYT
                     ETPVRVSGFKRVIDEQDWTITKVTHFLNNSGFTTSLELEVRLSDVEYETEDDE"
     gene            complement(31089..31568)
                     /locus_tag="N7B39_001225"
     CDS             complement(31089..31568)
                     /locus_tag="N7B39_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU2421240.1"
                     /translation="MMLALGMFVFMRQTLPHQTMQRESDYRWPSNSRIGKRDAYQFLG
                     VGEENMTLAGVLYPELTGGKLTMTTLRLMAEEGRAWPLLDGTGMIYGMYVISRVSETG
                     SIFFADGTPRKIDFTLSLTRVDEPLAALYGDIGKQAESLIGKAGSMATKFTGMTGAG"
     gene            complement(31583..34030)
                     /locus_tag="N7B39_001226"
     CDS             complement(31583..34030)
                     /locus_tag="N7B39_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU2421241.1"
                     /translation="MSNNVKLQVLLRAVDQASRPFKSIRTASKSLSGDIRETQKSLRE
                     LNGQASRIEGFRKTSAQLAVTGHALEKARQEAEALATQFKNTERPTRAQAKVLESAKR
                     AAEDLQAKYNRLTDSVKRQQRELAAVGINTRNLAHDELGLKNRISETTAQLNRQRDAL
                     ARVSAQQAKLNAVKQRYQAGKELAGNMASVGAAGVGIAAAGTMAGVKLLMPGYEFAQK
                     NSELQAVLGVAKDSAEMAALRKQARQLGDNTAASADDAAGAQIIIAKAGGDVDAIQAA
                     TPVTLNMALANRRTMEENAALLMGMKSAFQLSNDKVAHIGDVLSMTMNKTAADFDGMS
                     DALTYAAPVAKNAGVSIEETAAMVGALHDAKITGSMAGTGSRAVLSRLQAPTGKAWDA
                     LKELGVKTSDSKGNTRPIFTILKEMQASFEKNRLGTAQQAEYMKTIFGEEASSAAAVL
                     MTAASTGKLDKLTAAFKASDGKTAELVNIMQDNLGGDFKEFQSAYEAVGTDLFDQQEG
                     ALRNLTQTATKYVLKLDGWIQKNKSLASTIGLIVGGALALIGIIGAIGLVAWPVITGI
                     NAIIAAAGAMGAIFTTVGSAVMTAIGAISWPVVAVVAAIVAGALLIRKYWEPVSAFFG
                     GVVEGLKAAFAPVGELFTPLKPLFDWLGEKLQAAWQWFKKLIAPVKATQDTLNRCRDT
                     GVMFGQALADALMLPLNAFNKLRSGIDWVLEKLGVINKESDTLDQTAARTHAATYGTG
                     GYIPATSSYAGYQAYQPVTAPAGRSYVDQSKNEYHISLTGGTAPGTQLDRQLQDALEK
                     YERDKRARARASMMHDG"
     gene            complement(34023..34178)
                     /locus_tag="N7B39_001227"
     CDS             complement(34023..34178)
                     /locus_tag="N7B39_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HGU2421242.1"
                     /translation="MTFPKNLSVDDLMADVAVVFHWPPSELYPMSLTELITWREKALR
                     RSGNTNE"
     gene            complement(34175..34450)
                     /locus_tag="N7B39_001228"
     CDS             complement(34175..34450)
                     /locus_tag="N7B39_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HGU2421243.1"
                     /translation="MNKENVITLDNPVKRGEQVIEQVTLMKPNAGTLRGVSLAAVANS
                     EVDALIKVLPRMTAPMLTEQEVAALELPDLVALAGKVVGFLSPNSVQ"
     gene            complement(34507..35025)
                     /locus_tag="N7B39_001229"
     CDS             complement(34507..35025)
                     /locus_tag="N7B39_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HGU2421244.1"
                     /translation="MAMPRKLKLMNVFLNGYSYQGVAKSVTLPKLTRKLENYRGAGMN
                     GSAPVDLGLDDDALSMEWSLGGFPDSVIWELYAATGVDAVPIRFAGSYQRDDTGETVA
                     VEVVMRGRQKEIDTGEGKQGEDTESKISVVCTYFRLTMDGKELVEIDTINMIEKVNGV
                     DRLEQHRRNIGL"
     gene            complement(35038..36228)
                     /locus_tag="N7B39_001230"
     CDS             complement(35038..36228)
                     /locus_tag="N7B39_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HGU2421245.1"
                     /translation="MSDYHHGVQVLEINDGTRVISTVSTAIVGMVCTASDADAETFPL
                     NKPVLITNVQSAIAKAGKKGTLAASLQAIADQSKPVTVVVRVEDGTGDDEETKLAQTV
                     SNIIGTTDENGQYTGLKALLAAESVTGVKPRILGVPGLDTKEVAVALASVCQKLRAFG
                     YISAWGCKTISEVKAYRQNFSQRELMVIWPDFLAWDTVSSTTATAYATARALGLRAKI
                     DQEQGWHKTLSNVGVNGVNGISASVFWDLQESGTDADLLNESGVTTLIRRDGFRFWGN
                     RTCSDDPLFLFENYTRTAQVLADTMAEAHMWAVDKPITATLIRDIVDGINAKFRELKT
                     NGYIVDATCWFSEESSDAETLKAGKLYIDYDYTPVPPLENLTLRQRITDKYLANLVTS
                     VNSN"
     gene            complement(36288..36881)
                     /locus_tag="N7B39_001231"
     CDS             complement(36288..36881)
                     /locus_tag="N7B39_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HGU2421246.1"
                     /translation="MLIGYVRVSTNDQNTELQRNALECAGCELIFEDKISGTKSDRPG
                     LKKLLRTLSAGDTLVVWKLDRLGRSMRHLVILVEELRERGVNFRSLTDAIDTSTPMGR
                     FFFHVMGALAEMERELIVERTRAGLEAARAKGRIGGRRPKLTTSEWEQVGRLLAAGES
                     RQRVALIFDIGLSTLYKKFPSSATKNKLCHPLANRDK"
BASE COUNT         8321 a        10041 c         9729 g         8851 t
ORIGIN      
        1 cttaaataat gttttattat taccactttt aattcaggga taatgtgagg ttattaccct
       61 caaataatat gagataaata gtgctgcaac attgcatttt tgccccgatt tatccatgat
      121 cgaattgtga catttgtcat acaacgaata ggttttgtac ttactatgga atggattgcc
      181 gatccgtcta tctgggccgg gttaatcacg ctgattgtga tcgaactggt cctcggcatt
      241 gataacctgg tctttattgc catcctcgcc gaaaaactac cgccgaagca gcgcgaccgc
      301 gcacgggtta ccgggctgtt gctggcgatg ttaatgcgcc tgttactgct ggcgtcaatc
      361 tcctggctgg tcaccctgac tcaaccgctg ttcagcttcc gctcgtttac ctttagcgcc
      421 cgcgacttaa tcatgctgtt tggtggtttc ttcctgttgt tcaaagccac gatggagctg
      481 aacgaacggc tggaagggaa agacagcaat aatcccacac aacgcaaagg tgcgaagttc
      541 tggggggtgg tgacacaaat tgtggtactg gacgccatct tctcactcga ctcagtgatt
      601 accgccgtcg ggatggtcga ccatttactg gtcatgatgg ccgccgtggt tatcgctatc
      661 agcctgatgt tgatggccag caagccgtta acgcaatttg ttaacagtca cccgaccatc
      721 gttattctct gcttaagctt cctgttgatg attggcttta gcctggtggc agaaggtttc
      781 ggcttcgtca ttccgaaagg ctacctgtac gctgccattg gtttctcggt gatgatcgag
      841 gcgctcaatc agttggctat ctttaaccgg cgacgttttc tttccgctaa ccagacactg
      901 cgccagcgga ctaccgaagc ggtaatgcgc ctgcttagcg ggcaaaaaga ggatgcggaa
      961 ctggacgccg aaaccgcgtc catgctggtg gatcatggta accagcagat ctttaatccg
     1021 caggaacggc ggatgattga gcgggtactt aatcttaacc agcgtaccgt cagcagcatt
     1081 atgacgtcgc gccacgatat tgagcatatc gatctcaacg cgccggaaga cgagatccgc
     1141 cagttgctgg agcgaaatca gcatacgcgg ctggtggtta ctgatggcga tgacgcggaa
     1201 gatttgctcg gtgttgttca cgttatcgac ctgttacaac aatcactgcg cggcgaaccg
     1261 ctcaacctgc gggtgttaat tcgccagccg ctggtgttcc cggaaacctt gccgttgtta
     1321 cctgccctgg agcagttccg taatgcccgc acacactttg cttttgtggt ggatgagttt
     1381 ggctcggtgg aagggattgt gacattaagt gacgtcactg aaaccattgc cggtaactta
     1441 ccgaacgaag tggaagagat cgacgcccgc catgatattc agaagaatgc ggacggttcc
     1501 tggacggcga atggtcatat gccgctggaa gatctggtgc aatatgtgcc gctgccgctg
     1561 gatgaaaaac gtgaatatca caccattgcc gggctattga tggaatattt gcagcgtatt
     1621 ccaaaacctg gcgaagaagt tcaggtgggg gattatttgc ttaaaacgtt gcaggtggaa
     1681 agccatcgcg tgcagaaggt gcagattata ccgctgcgta aggatggcga gatggagtac
     1741 gaggtgtgat aaaacatcaa atgcgcaggt tcaaactgcg catttgattt atacaaaatt
     1801 tagtataatc taaggatgga gtaatgagaa aaaagctggc ttttcttgat accagtcttg
     1861 acgatttacg cgcatttcca gaatcgtctc gacaggaaat tggctatcaa ctcgacagga
     1921 tccaacaagg gttaaaccca tacgactgga aacctttttc aactatcggc cctggtgtgc
     1981 gagagattcg tactcgcgat gctgatggga tctatcgcgt catgtatgtc gcaaaatttg
     2041 aagaagcggt ttacgtattg cattgttttc aaaagaaaac acagactacc agtcaatcgg
     2101 atattgattt agccaaacga cgctataaag aacttgttca ggagcggaaa aatgaaaatt
     2161 gaaacctttg atagtgtgtg ggatgcggtg agtgacaccc ctgaacaagc ggaaaatatg
     2221 cggatccgtg ccgagcttgt aacgatcatt aataactgga ttgaacaaca agggtttagc
     2281 caggctcagg cagcttctgc tttaggggtc actcaacctc gcatctctga acttgccaga
     2341 ggtaaaatcc agatctttag tatcgataaa ttgatcacca tgatggctca tgcaggacta
     2401 catattcaac gtattgaagt tcaatatcca catgctgctt agtgctataa atacagatgg
     2461 cgacaaattg atgccgccat ctatataact tgtcttacat cttctccagc aacttcttca
     2521 catctttgcc attgcgggaa tctttattcc gctcggccca gtcattcagg cgacgtttcg
     2581 cttcgtcctg tagatgtttg cgcagtaact gatccacttg cagactgtaa ttgagttgct
     2641 gccagttgcc ataaacccgc agcggtactg gcgtttcttt gaggaaatca atcagtttgc
     2701 tttccccgtt ccagccaccc acgacccgaa tatcaaactg ggtgtcgcag gtttgagctg
     2761 ccagattcaa catgccttcc cctgtaagcg ccagcacagg cgattgacct tgcatgtcgt
     2821 ttaacgtcac gacgccatcc ttcaacgtca aatcggtggt aaagcggtca agacgcgtta
     2881 cgttatcgaa gttttcagcg gccttcacat caccaccatt acgttctacc gcttgctgaa
     2941 tcatctgctg gaagttcatc ccttccatgc gcgtatcggc catttcgaca tgtgcctgtc
     3001 cttgccagtt gtggcggaat gcgtcggcat ctatgtcagc accggagaaa tcaccagcca
     3061 atgacatttt tccggtcaac gaaatcggat agttaaacgc cttcagaatg gtgccaatct
     3121 caacgttttc cagccgtggc tggaagttta tccgcggatt tattgatgtc gcgtccagcg
     3181 tgcccggcag tgaaacctgt ccaccgttaa gcttgccctg tagttgagta atttccagca
     3241 aacccgactt gttggtcatt tgcgtggcaa catctgtaaa attcattccg cgccagcgca
     3301 cgttactggc ctgcaacaaa atatcagccg taaagccttg cagtccctga taggccggtt
     3361 catcaatacg cgaagaaata accgggcgcg gcaacgttga ttggctctgc ccctgctgcg
     3421 cggcaccgtt ttcaccattc gcggtttcat taagcgggat gaggttgtca agattcagtt
     3481 gcgggaattg cagcctcagc tgccattccg gtttctctgt cagcgtgacc tgtgcttgcc
     3541 cgctcagcgt actatcattg gcggtcaaac taatttggtt aaatgaaagg cgtttacgtg
     3601 actcctgcca ctgggcctgg aaactcccct gcccctgaat accttgtttt ggtaaatcgg
     3661 caccctgcaa ctgccagtta atttgttcaa tagccgccgt taaatcatgc ggataatcag
     3721 aagcatctac cgtaccgtta agggatatcg tgagatcgcg ctgatcgcga ttaacccgcc
     3781 cggagaactc aaatgagcca cgatgttggg gatcttgttc catttgcagg cggatattgc
     3841 ggattgtcac ctgctcgtca tcttcatgct ggaacaccag cacgctgtcc gccaccttaa
     3901 gactggatat atcaaacgac catccgcgat cgtctgacag atccggcaag gtattgtcgc
     3961 gcggtgcaac cggagcgtct tcactgcgca ccgcttccgt ctgcggcgtc agttggatca
     4021 ctgccccttt tagcatcacc tgcttaacgc tcagttgatg actcagtagt ggtaaaagcg
     4081 ccacgtccag gcgcatgttg tcggcgcgaa ccagtggctg gctcgcgccc tgggcggtga
     4141 gagacattcg cccggagagg atactaagct gcggccagac gtgccaacgc agtggcccgt
     4201 cgagctgcaa ttgataaccg ctacgtgcag caacttgctt gaccatatag tcgcggaaat
     4261 cattcggatt caccagcaac actaacgcag ataacccggc caccagcacg accaggagta
     4321 tcatcagcgt cgtcagaaat cgtctcatgg tatcctcaat gggcctgaat tagtctttat
     4381 cgattcggct ggctaccgcg ccctgctggt tgcgatactt cgcatcttca cggcggttgt
     4441 aaggtcgcgc cgccgggccg gaaagcggct caaagctcag cgcaccaatt aacatgcccg
     4501 gacgcagcgc cagcggcagc ttaccggagt tgtagaactc cagcacaatg caaccagacc
     4561 agcccggatc gatacggtgc gcggtgacgt gcaccatcag ccccagacgc gccagtgagg
     4621 aacgcccgtc cagccagccc accagatcgg ctggcagcgt caccgactcc agcgtcaccg
     4681 ccagcgccag ctctcctggg tgaagataga acgcctcgct ttcatcgaga acgatctcat
     4741 cgctcatcac gcggtcaagc gcggcgctca cttcatcttt gggaccgctc agatcgataa
     4801 acgctgccgt gtgaccacgg aaggtacgaa atttattgcc caggcgtaca tccaccgtcg
     4861 cgccgttaat acgctccact ggcggacgtg ggttgatcga caaacggcct tcatcaagcc
     4921 aggcttcaat atctcggtca cacagacgca tggcattttc tcctttcgcg catcactccc
     4981 ttaacgccaa ttacgtcaag ggcataccag gttatcactg aacggtacac aatttatcaa
     5041 gcttattcaa agaactgact tattttcgct ttcaatatat cgatcgcgat gcggtttttc
     5101 ccgccgcgcg gcacgataat gtccgcatat tgtttagaag gctcaatgaa ttgcaggaac
     5161 atcgggcgca cggttttttg atattgcgcc atcacggaat ccattgaacg cccacgctcg
     5221 ttaacgtcac gcttgatgcg gcgcatcagg cagatatcca gcggggtatc aacgaaaatg
     5281 gagaagttaa gttcgtcacg caaacgtgca tccgtcagca acaaaatgcc ttcgagaatg
     5341 atgaccttct tcggctcaac cgtcaccgtt tctttcatac gcgtatgttc aacatagctg
     5401 taaaccggca ggtcaattgc cgagccgcgt ttcaacgctt gtaaatgctc aagcagcagg
     5461 ctgtgatcca tcgcgctggg atggtcgtag ttggtcttaa cgcgttcttc catcgacaga
     5521 tggctttgat ctttgtaata gcagtcttcg ggaattacgc cgatgtgttc atcaccgact
     5581 tgctcacgta attcacgata aagggtactg gcaataagac tcttgccgga agccgatgcg
     5641 ccagcgatac cgataatgac gcactgatga gactgatcag tcatatattt agcgacctga
     5701 ttaacctgga tgttaggaag ggggcgacga agcgccaaac gcggcaatta tagggatttc
     5761 atccgcctga taccagtcga atagcgttgc cgcgcgctca gagttaattg ttgacgaaga
     5821 attcccggtg gcaaattacg ttgatcagtt ttatacaagg taaaaattgt tatacgcagt
     5881 tgcgcaaatt atccgccttt acgtcacttt atgagcaatt cgcatataaa atgtaaaact
     5941 tttgtactag cataaacaca gaaacgaata ctggcgacct ggccttgcgg ataaagcggt
     6001 aatgagcaaa caatcacagc atgtattaat tgccctgccc cacccgctgc ttcacctggt
     6061 cagtttaggt ttagtctcgt ttatcttcac ccttttctcg cttgagcttt cgcagtttgg
     6121 cacccaactc gccccactgt ggttcccgac gtccatcatg atggtggcgt tttatcgcca
     6181 tgccgggcgc atgtggccag ggattgcgct gagctgctcg ctgggaaata tcgccgcatc
     6241 catcctgctt ttttccacca gctcgctgaa catgacctgc acgaccatca atattgttga
     6301 agccgtggtc ggggcagtgc tgctacgtaa attgctgccg tggtataacc ccttgcaaaa
     6361 tctggctgac tggctgcgtc tggcactcgg cagcgccatt gttccacctc tgttgggggg
     6421 tgttctggtt gtcctgctga cgcccggaga cgatcctcat cagggcattt ttgatatggg
     6481 tactgtcaga atccatcggc gctctggcac tggtgccgct gggattgtta tttaaaccac
     6541 actatctgct gcgccatcgc aacccacggt tgctttttga gtcgctgctc acgttagcca
     6601 tcacactgac gttaagctgg ctttcgatgc tgtatctgcc gtggcctttt actttcatta
     6661 ttgtgctgtt gatgtggagc gccgtgcgcc tgccacgaat ggaagccttt ttgatcttcc
     6721 ttaccacggt gatgatggtg tcgctgatga tggccgcgga tccctccctg cttgctacgc
     6781 cgcgtacgta cctgatgagc catatgccgt ggctaccgtt tttgctgatc ctgctgcccg
     6841 ccaacatcat gacgatggtg atgtatgcct ttcgtgcgga acgcaaacac atttccgaaa
     6901 gcgaaacccg ttttcggaac gccatggaat attccgccat cggcatggca ttagtgggca
     6961 ccgagggaca atggctgcaa tccaacaaag cactctgcca gtttctcggt tacagtcagg
     7021 aagagctgcg cggactcacc tttcagcaac tgacctggcc ggaggatctc aataaagatc
     7081 tccaacaggt tgaaaagctg ataagcggtg aaataaacac ctattcaatg gaaaaacgtt
     7141 actacaaccg caatggcgat gttgtctggg cgttgcttgc cgtctctctg gtgcgccaca
     7201 cggatggcac gccgctctat tttatcgctc agattgaaga cattaacgag ctaaaacgca
     7261 ccgaacaggt gaatcagcaa ctgatggaac gcatcacgct ggctaacgaa gcgggcggga
     7321 ttggcatctg ggagtgggaa ctgaagccga atatttttag ctgggataag cggatgttcg
     7381 agctgtatga aattcctccg catatcaaac cgaactggca ggtgtggtac gagtgcgtgc
     7441 tgccggaaga tcgccaacac gccgaaaaag tgattcgtga ttcgttgcaa tcacgctcgc
     7501 cctttaaact ggaatttcgc attgccgtga aagatggcat ccgccatatc cgcgccctcg
     7561 ccaaccgggt actgaataaa gaaggcgaag tcgaacgcct gctcggcatt aatatggata
     7621 tgactgaggt taaacagctt aacgaggcat tgtttcagga aaaagagcgc ctgcacatta
     7681 cgctggactc catcggcgaa gccgtggtct gtattgatat ggcgatgaaa attaccttta
     7741 tgaatccagt ggcggagaag atgagcggct ggacgcagga agaagcgtta ggtgttccgc
     7801 tcctgacggt gttgcatatt acttttggcg acaacggacc attaatggag aacatttaca
     7861 gtgccgacac ctcacgttcc gcgatcgaac aagatgtggt gttgcactgt cggagcggcg
     7921 gcagctacga cgtgcattac agtattacgc cgttaagtac tctggacggc agcaatatag
     7981 gctcggtact ggtgattcag gacgttaccg aatcacgcaa aatgctgcgc cagctgagct
     8041 acagcgcctc ccatgatgca ctgacgcatc tcgccaaccg cgccagtttt gagaaacaac
     8101 tgcgtatcct gctgcaaacg gtaaacagta cgcatcagcg acatgcactg gtgtttatcg
     8161 atcttgatcg ctttaaagcg gtgaatgaca gcgccgggca tgcggcgggc gacgctttgc
     8221 tgcgcgaact ggcgtcgttg atgctgagta tgctgcgctc cagtgacgtg ctggcgcggc
     8281 tcggcggtga tgaatttggt ctgctgctgc cagactgcaa tgttgaaagc gctcgtttta
     8341 tcgctacacg tattatcagt gctgtgaatg actatcactt tatatgggaa ggccgggtac
     8401 atcgggtagg tgccagcgcc gggattacct tgatcgatga caacaatcat caggcggcag
     8461 aagtgatgtc gcaggctgat atcgcctgtt atgcctccaa aaatggtggc cggggccggg
     8521 tgacggttta cgaaccgcag caagctgccg cacatagcga acgggcggcg atgtcgcttg
     8581 atgaacagtg gcggatgatt aaagagaatc agttgatgat gctcgcccac ggtgtcgctt
     8641 cgccacggat cccggaagcg cgtaatttgt ggctgatttc acttaagctc tggagttgcg
     8701 aaggcgagat tattgatgaa caaacatttc gtcgtagctt cagcgatccg gcgcttagcc
     8761 atgctcttga ccgccgggta ttccacgaat ttctccagca ggccgcaaaa gcggttgcca
     8821 gtaaaggcat aagcatcgcc ctcccccttt ccgttgccgg tttgagtagc gccacgctgg
     8881 tgaatgatct gcttgagcag ctggaaaata gccctctacc accacggtta ttacatctga
     8941 ttattccggc agaagcgatt ttcgatcacg cagaaagcgt gcaaaaactg cggctggggg
     9001 gatgccggat cgtattcagt caggtgggcc gcgatctgca aatcttcaac tcgctgaaag
     9061 cgaatatggc agattacctg ctacttgatg gtgagttatg cgccaacgtg cagggtaatt
     9121 tgatggatga gatgctgatt acgattattc aggggcacgc tcagcgactc gggatgaaaa
     9181 ccatcgccgg gccagtcgtt ttacccttag tgatggatac gctttctggc atcggcgtcg
     9241 atctgattta tggtgaggtg attgccgatg cccaaccgct ggatttgctg atgaatagta
     9301 gttatttcgc gattaactga gggagcgttt gcccattgcc tgatgcgacg ctaacgcgtc
     9361 ttatcaggcc tacaaatcgc tcattccgca aggcgctcgc accgcatccg gcgaccaacg
     9421 tcatgcttcg tctggttgcc agccttccgt ataccagata tgcaacagcg cataagaacg
     9481 ccagggcttc cagcgctcgg cataacggcg gatttgcgcc ggtgtcattc ccggaaatcg
     9541 ctgtttaatc agataatcat ccggcaggaa aacatctttc gcctgccagc cacgcaaagc
     9601 aaaataattc gccgtccagc gcccgatacc cggaaaagtt tgcagcgttt tcatcgcctg
     9661 ctccacatcg cccggtattg tcattggtaa ggtgccctcc agcgccgcat ttgccagatg
     9721 aatcagcgcc tctgcccgtt tcaacggcat acctaacgct tttaatgcct gcgggtctgc
     9781 tgctgccagc cgctgaggcg tggggaagca gatatactcc ggaaaatcat ccagccgttc
     9841 gccataaagc tgtaccactt tggcagtcaa ttttgccgcc atcgccacgc tcaccagttg
     9901 gcctaaaatc gcccgcacgc cttgctcaaa agcatcaaca ctgccgggta aacgcaatcc
     9961 gggccgcgcc gcgcctaact tgcccaacgc accgttaaca atctgcgggt tacattgcag
    10021 atcaagcagg cggctcattt tcgccagaca ctcagcggca acgggttcta aatctgcact
    10081 taaatttatg tgcagagtat ggcgggctat atccggaata gcagtcacca cgccgcgata
    10141 ttcgcccacc gccagactac gggcataata atcgtcagcg accgtttcca caccactcac
    10201 tgcacgggcg gcgagaaatc ccaacatcca cgaccagtca tacggcggct gccagttcag
    10261 ggtatacatc gcatctcctt attcatccgc tttcagcata aacgttattc agacgctgcg
    10321 ctttgctttc atattccggt tgtcgcgacg gcaacatttc gctaaagtca cgccccttct
    10381 tcaccggcat ggggattatt tcgtgtttat tggttttgat tacggtacag caaactgttc
    10441 agtagcggtc atgcgtgacg gtaaaccgca cttgctaaaa atggaaaacg acagcacgct
    10501 gctgccttca atgctttgcg cgccaacgcg tgaagcggta agcgaatggc tgtaccgcca
    10561 tcatgatgtt ccggcagacg acgatgaaac gcaggcactg ttgcgtcggg cgattcgtta
    10621 taatcgcgaa gaagatatcg atgttacggc gaaaagcgtg cagttcggtc tttcctcact
    10681 ggcacagtac attgatgatc cagaagaagt gtggtttgtg aaatcaccaa aatcgttcct
    10741 cggtgccagc ggcttaaaac cgcagcaggt agcgctgttt gaggatctgg tctgcgcaat
    10801 gatgttgcac attcgccagc aggcgcaagc gcagctgcca gaagcgatta ctcaggcggt
    10861 gattggtcgc ccgatcaact tccaggggct gggcggcgat gaagcaaacg cccaggcgca
    10921 agggattctg gaacgcgcgg cgaagcgtgc cggattcagg gacgtggtat tccagtacga
    10981 gccggtagcg gctgggctgg attacgaagc caccttacag gaagaaaaac gggtactggt
    11041 ggtggatatc ggcggtggta cgactgactg ttcattgctg ctgatggggc cgcagtggcg
    11101 ttcgcgtctc gatcgtgaag ccagcctgct gggtcacagt ggttgccgta ttggcggtaa
    11161 cgatctggat atcgcactgg cgtttaaaaa cctgatgcca ctgctgggta tgggtggcga
    11221 aaccgaaaaa ggcatcgccc tgccgattct gccgtggtgg aatgcggttg ccatcaacga
    11281 cgtacctgcg cagagtgatt tctacagtag tgccaacggt cgtctgctta acgatctggt
    11341 acgcgatgcc cgcgaaccgg aaaaagtggc cctgctacag aaagtctggc gtcagcgttt
    11401 aagctatcgc ctggtgcgta gcgcagaaga gtgcaaaatt gcgctttcaa gcgtagcgga
    11461 aacccgcgcc tcactgccgt ttatcagcga tgaactggct acgctgatta gccagcaagg
    11521 gctggaaagc gccctcagcc agccactggc gcggattcag gaacaggtgc aactggcgct
    11581 ggataacgcc caggaaaaac cggacgttat ctacctgacc ggcggaagcg cccgttctcc
    11641 gctgattaaa aaagcgctgg cagaacagtt gccgggcatt ccgattgcag gcggcgatga
    11701 ctttggctcc gtcaccgccg ggctggcacg ctgggcggaa gtggtgtttc gttaattgaa
    11761 aaatggctca gcagcggctt aaatctgcct gcgcctgagc cagtttttct gccgcctgac
    11821 gtaacggttc catcaacggc atggtccgtg ctggagcatg aaggcggaat cgctgcaact
    11881 ctgtcgcgcc gacagtcaac gccgattcca gccgatgtcg tttggcggcc attttcgcca
    11941 ttaccgcttg tctttctgca ggcgttaccg cttcgttcgg cctgaacaca aaacggcgtt
    12001 cacagctcgc tttccagtcg ataaccgcct gggtcagatg atcaccaaac cctttaactt
    12061 gcttaacccc acgacgggta acatccgcgg ctgtttcaat accaaaagac cgtaacgccg
    12121 ctttacgcgc agggccaacg ccgggaatag aggcagcatc aataaaaaat ccctccagaa
    12181 acttctgctt ctgccgttcc cttgcagtgt catgaagtgc tgccagagcg cgtttctcct
    12241 cttccggtaa cccgagaatt tcgtccttca ttttttcgag catcgcccgt ttggcgataa
    12301 aaccttccag cccgcccaac tgttggatct ggctgactaa atggtcatag tccattttcg
    12361 cgcgattaaa tgcctcacgg cgttgctgga tttctgcttt ataaactttg cttgtcagac
    12421 tgccaataat ccagatagcc gccagcacag gaaccaaaat aatataacgc ggttctgcct
    12481 gaaggccgca aagcaacagc gataacgctg acagtgcgat ctcaatcaga atgatgtatt
    12541 cgcgccgtaa caggcctaaa ggaagcggcc tgccagtcgg ttggaaatga tcgggtaatg
    12601 gcaattgcaa tgctggcggt gctactgacg ccatcaccat cgcccagact ttcgccagca
    12661 caaaatcacc actggtggta atgacctctt cgccgagatc aataaaatag ataacgcctt
    12721 gattatccag cgcacaccac gggcagtcag tcaaatgacc ggggtaaaca tgcattgccg
    12781 aaacggtaca tttctttaac tgttggcgta gtaaatccag tgccgctacc cacgcttttg
    12841 ccgtcggacg cgcggttgcc acgccacttt ccgtgaatgc ctgctgaaac atggcttcaa
    12901 catcgcccgg taacatcgac agcggaatcg atcgtggcgg cggttttaaa ccacgtcgct
    12961 ggttgtctga cgcgtaggca taacggaaat gggcaatatc cgtctccagc gcattacccg
    13021 catcagagat aagcggcaca ccagaataag gatgccgccc accaaacaag acgtgaaaaa
    13081 tcagcaacgc aaggccaaaa ttatcgtgat tcgcggtacg ttcaaaaccg acaaatgatg
    13141 gcaaggtttg tagctctggc ggcgtaaaat gcgacacgcc gacttcgcat aaatgcagtg
    13201 tgccattggc gttaatctga aaggagtcac tatcgatcaa caccactttg ctgtcgcgac
    13261 ctaccataaa gctgttctgg tttacgtccc ccacgacgtg cccgtgctcg tgaaccgtag
    13321 caaaagatga agcaatattg cgcgcaacat agagtagaaa atcccacgca caatgaggat
    13381 aactctggcg acgatgtgcc gggctataga tcatatgaat cggttcttta ccagaaactt
    13441 ttggcatcat aaaaccgata acttttccgc ctcgtccacc gtgaagcgtt gcctgcggcc
    13501 aggcgacata attcaacaac tgcgcgtcag ctgtcgcagc cataaaggca agtttgtcct
    13561 gttttaaggc gggtggcggc gtgtgataaa tcttggcgac gctatcgaca aactcattga
    13621 tatcataaac cgcgccttcg ccacctttgc ccagttcacg gcctggcgta acgcactcac
    13681 cagtagcagt atataaagtg ggtttcatgg taaccacagc gccagtgtca acgttttatc
    13741 atcatcggta cgctcgttta ccgctgggct cgacaaaaac tgctttaaca attcaggtaa
    13801 taaatcgagt tgttcctgcg ttgctgccgc cagtccattg aaaaacgggg taaagaaagg
    13861 cacatgcgga gaattatcca gcatatttaa cgccagtcgc tgaataccat ccgtaaatgc
    13921 tgcaacttta tgcgcacgct cagtgctggt gaaagtttcc agtctggaaa cgacatcttc
    13981 atcggtaatg aaatgcgtca tattggcata ttcacccacc atcggcgtaa gcggtaattg
    14041 cagaccgtga ccaaaatcca ccaccacgcc gccatcgcca atctgcataa tcaaggtgcc
    14101 gttagccgat gaaatcaatc ccagaaacgt gcaggcaaaa tcgcgaactg ctaattcttt
    14161 agcttccgcc tcggcaaaaa gtcgctgacg aacggtcagc acaatatccg tggcgagaat
    14221 atcattgagt ccaaattcac cgtgctgcac tttttgcgac acataagcca tcgcttcatt
    14281 gatggcaagc atcgcgcctt cgccaccctg cgagacactg cctgcgccat cagcaaggaa
    14341 catcaccagt aatggctgct ggtcatttag ccaggcaacc tgcatctggc aggcatcctg
    14401 acaaggtaaa tcagcgctaa tgtgcgacgt tccaacagcg gatgcataaa ccagacgcca
    14461 gctcacactg acgtccagcc ttttggcgct tccagcacaa cttccgttcc cggcgtggag
    14521 cgggaaaccg aacgcagcga actggataac cagctaaaca gttcacggaa ttgtaatccc
    14581 tgcagtggca aaggctgacg aacgctgatt tgtgccagag tcttaatatc cgccccctga
    14641 acaccgatgg aaaagaaggc aaacttctta tcttcttccc cctgaaacac tttgttggcg
    14701 gcagcctgcc actcatcggt tggtgcacca tcggtgatca ggaaaatcca cgggcgataa
    14761 tacgaaatac catttgcacg atactcacgt ttccgctcct ctaccatatc cagggctttg
    14821 gtaatggcgg cacccattgg tgtatccccc tgggcaaaca ggatgggcgg gaaaaaatta
    14881 gcggcgctgg taaagggttg ttccacatgc accgggccga acgtcacaat accaagttcc
    14941 actcttttta aggccagcgg gtcggcaagc agctcatcgc gaaaggtaac caatccggcg
    15001 ttaagttcat taatgggtct accgttcatc gaaccagaaa catccagcag caaaatgcag
    15061 gggcaacgtg gttccgggtt actggcaaaa tcgctggtgg caaacgtgat ttgttcgctc
    15121 ataagaaatc ctttcttaga gaattattga ggtccggtat cataccgatt gcaataatga
    15181 aataagagca aacgaagaaa ctttcagaat taggcccggc ggcgcagaaa attttgctat
    15241 tcgcctcgca agaacggaga gccgtcgatt gacactaagg gcggagtgac ataatttcag
    15301 gagtgagggt tagggaaagg tttccccctc cccctggtgt tcttagtaag cctggaagct
    15361 aatcactaag agtatcacca gtatgatgac gtgcttcatc ataacccttt tcttatttaa
    15421 ggccccttcc tcgggagggg ctttcccgtt tcagcatccc gctgaaatcg tcggcttacc
    15481 tcctttcgcc atgcatgcag tctatcgctg acttgcggat aaaatagttt ccagtgtgat
    15541 tattgcgtaa cgtctcgcaa agaatatttg ctggcaggat cgcagactgc aaagcctgca
    15601 gattgacaat cttatcgtga aggcatactt tcaggagtga gggtagagcg gggtttcccc
    15661 cgccctggta gtcttagtaa gccgggaagc ttatgactaa gagcaccacg atgatgatcc
    15721 acttcatcat gaccctttcc ttatttaagg ccccttcctc gggaggggct ttcccgtttc
    15781 agcgtcccgc tgaaatcgtc ggcttacctc ctttcgccct gaattcagtc tatcgctgac
    15841 ttgcggataa aatagtttcc tctgtgatta ttgcgtaacg tctcgcaaag aatatttgct
    15901 ggcaggatcg cagactgcaa agcctgcgga ttgacattct gaacgtgaag gcatactttc
    15961 aggagtgagg gtagagcggg gtttcccccg ccctggtagt cttagtatgc tgggtagcta
    16021 atcactaaga gcaccacgat gatgagtatc ttcatcatga ccctttcctt atttatggcc
    16081 ccttcctcgg gagggggctt tcccgtttca gcgtcccgct gaaatcgtcg gcttacctcc
    16141 tttcgccatg caagcagtct atcgctaacg cgtagataaa atagtttcct gtgttattac
    16201 tggatgcgtg ctcgcaaatg tgcccgccat tcagacgatt cccgatagtg tttcataatt
    16261 cctccatttt tctcccttat tggctggcta cactagtatc attccgcgaa acgtttcagg
    16321 aagagaaact cttaacgatg aaaggcagtt ataaatcccg ttgggtaatc gtaatcgtgg
    16381 tagttatcgc cgccatcgcc gcattctggt tctggcaagg ccgcaatgac tcccagagtg
    16441 cagccccagg ggcgacgaaa caagcgcagc aatcgccagc gggtggtcgc cgtggtatgc
    16501 gttccggccc attagccccg gttcaggcgg cgaccgccgt agaacaggca gttccgcgtt
    16561 acctcaccgg gcttggcacc attaccgctg ctaatactgt tacggtgcgt agccgcgtgg
    16621 acggtcaact gatggcgtta catttccagg aaggccagca ggtcaaagct ggcgatttac
    16681 tggcagaaat tgaccccagc cagttcaaag ttgcattagc acaagcccag ggccaactgg
    16741 caaaagataa agccacgctt accaacgccc gccgtgatct ggcgcgttat cagcaactgg
    16801 caaaaaccaa tctcgtctcc cgccaggagc tggatgccca acaggcgctg gtcagtgaaa
    16861 ccgaaggcac cattaaggct gatgaagcaa gcgtcgccag cgcgcagctg caactcgact
    16921 ggagccgtat taccgcacca gtcgatggtc gcgttggtct caagcaggtt gatgttggta
    16981 accaaatctc cagtggtgat accaccggga tcgtggtgat cacccagacg catcctatcg
    17041 atttgctctt taccctgccg gaaagcgata tcgctaccgt cgtgcaggcg caaaaagccg
    17101 gaaaaccgct ggtggtagaa gcctgggatc gcaccaactc gaagaaatta agtgaaggca
    17161 cgctgttaag tctcgataac caaatcgatg ccactaccgg tacgattaaa gtgaaagcac
    17221 gctttaataa tcaggatgat gcgctgtttc ccaatcagtt tgttaacgcg cgcatgttag
    17281 tcgacaccga acaaaacgcc gtagtgatcc caacagccgc cctgcaaatg ggcaatgaag
    17341 gccattttgt ctgggtgctg aatagcgaaa acaaggtcag caaacatctg gtgacgccgg
    17401 gcattcagga cagtcagaaa gtggtgatcc gcgcaggtat ttctgcgggc gatcgcgtgg
    17461 tgacagacgg cattgatcgc ctgaccgaag gggcgaaagt ggaagtggtg gaagcccaga
    17521 gcgccaccac tccggaagag aaagccacca gccgcgaata cgcgaaaaaa ggagctcgct
    17581 cctgatgcaa gtgttacccc cgagcagcac aggcggcccg tcgcgcctgt ttattatgcg
    17641 tcctgtggcc accacgctgc tgatggtggc gatcttactc gccgggatta tcggttatcg
    17701 cgccctgccc gtttcggcgc tgccggaagt ggactatccg accattcagg tggtcacgct
    17761 ctacccaggt gcgagcccgg atgtcatgac ctctgccgtt accgcgccgc tcgaacgcca
    17821 gttcgggcag atgtctggcc tgaaacagat gtcgtcgcaa agttccggcg gtgcgtcagt
    17881 tatcacttta cagttccagc taacattacc gctggatgtc gccgagcagg aagtgcaggc
    17941 cgcgattaac gctgcgacca acttgttgcc gagcgatctg cctaacccgc cggtttacag
    18001 caaagtgaac ccggcagatc cgccgatcat gacgctcgcc gtcacctcaa cagccatgcc
    18061 gatgacgcaa gtggaagata tggtggaaac ccgcgtcgcg cagaaaatct cgcagatttc
    18121 cggcgtcggc ctggtgacgc tttccggcgg ccagcgtccg gctgttcgcg tcaaacttaa
    18181 cgctcaggcg attgccgccc tcggcctgac cagcgaaacc gtgcgcaccg ccattaccgg
    18241 cgctaacgtt aactcggcta aaggtagcct tgacggccct tcccgtgcgg ttacgctttc
    18301 cgctaacgac cagatgcaat ccgccgaaga gtatcgccag ctaatcatcg cctaccagaa
    18361 cggcgcgccg attcgtctgg gcgatgtcgc aactgtagag caaggtgcag aaaacagctg
    18421 gctcggcgca tgggcgaata aagaacaggc cattgtgatg aatgttcagc gccagcccgg
    18481 tgctaacatt atctccaccg ccgacagcat ccggcagatg ctgccacagc tcaccgagag
    18541 tctgccgaaa tcggtgaagg tgacggtact ttctgaccgt accaccaata tccgcgcatc
    18601 cgtcgatgat actcagtttg aattgatgat ggctatcacg ctggtagtca tgattatcta
    18661 cctgtttttg cgcaatattc cggcgaccat cattcccggc gttgcagtgc cgctgtcgtt
    18721 aatcggcacc ttcgcggtca tggtgtttct cgatttttca atcaataacc tgacgctgat
    18781 ggcgttaact atcgccaccg ggttcgtggt cgatgacgcc atcgtggtga tcgaaaatat
    18841 ttcccgctat atcgaaaaag gcgaaaaacc gttggcggcg gcgctcaaag gcgcaggtga
    18901 aatcggcttt accattatct cgctgacctt ctcgctgatt gcggtgttga tcccactgct
    18961 gtttatgggc gatatcgtcg ggcgactgtt ccgcgaattt gctattaccc tggcggtagc
    19021 gattttaatc tcagccgtgg tgtcgctaac cctgacaccg atgatgtgcg cacggatgct
    19081 cagccaggag tcgttgcgta aacagaaccg cttctcccgt gcctcggaaa aaatgttcga
    19141 caggataatc gccgcctatg gtcgtggact ggcgaaagtg ctgaatcatc cgtggctgac
    19201 cttaagcgtg gcgctcagca cgctgctgct tagcgttcta ttgtgggtgt tcattccgaa
    19261 aggtttcttc ccggtacagg ataacggcat tattcagggc actttgcagg caccgcaatc
    19321 cagctccttt gccaatatgg cccagcggca acgccaggtc gcggacgtga ttttgcagga
    19381 tccggcagtg caaagtctga cctcatttgt tggcgttgat ggcactaacc cgtcgctgaa
    19441 cagtgcgcgt ttgcagatca acctcaaacc gttggatgaa cgtgatgatc gggtgcaaaa
    19501 agtcatcgcc cgtctgcaaa cggcggtgga taaagtgccg ggcgtcgatc tcttcctgca
    19561 accaacgcag gacctgacca tcgatactca ggtcagccgc acccagtacc agtttacctt
    19621 gcaggccacg tcactggatg cgctcagtac ctgggtgcca cagttgatgg aaaaactcca
    19681 gcagctgcca cagatttctg atgtctccag cgactggcag gacaaagggc tggtggcgta
    19741 tgtcaatgtt gatcgcgaca gcgccagccg tctggggatc agcatggcgg atgtcgataa
    19801 cgccctgtac aacgcgtttg gtcagcgact gatttccact atttatactc aggctaacca
    19861 gtatcgtgta gtgctggagc acaataccga aaatacccca ggcctcgcgg cgctggatac
    19921 cattcgcctg accagcagcg acggcggcgt ggtgccgcta agctcaattg ccaaaattga
    19981 gcagcgtttt gcgccgcttt ccatcaacca tctggatcag ttcccggtaa cgaccatctc
    20041 ctttaacgtg ccggataact attcgctggg cgatgcggtg caggcaatta tggacaccga
    20101 gaagacgctg aatctgccgg tggatatcac cacgcaattc cagggcagca ccctcgcctt
    20161 ccagtcggcg ctgggcagca ctgtctggct gattgtcgcg gcggtggtgg cgatgtatat
    20221 cgtgctcggc attctgtacg agagctttat tcacccgatc accattctct cgacgctacc
    20281 cacggcaggt gttggcgccc tgctggcgtt aatgattgcc ggtagcgaac tggatgtgat
    20341 tgcgattatc ggcattattt tgctgatcgg tatcgtgaag aagaacgcca tcatgatgat
    20401 cgacttcgcg ctggctgctg agcgcgagca aggcatgtcg ccgcgcgatg caatctacca
    20461 ggcttgtctg ttgcgttttc gtccgatcct gatgaccacc ctggcggctc tgcttggcgc
    20521 gctgccgctg atgttgagta ccggggtcgg cgcggaacta cgtcgtccgt taggtatcgg
    20581 catggtcggc ggcctgattg tcagccaggt gctgacgctg ttcaccacgc cggtgattta
    20641 tctgctgttt gaccgcctgg cattgtggac caaaagtcgc tttgctcgcc atgaagagga
    20701 ggcgtaagtg aagttttttg ccctcttcat ttaccgcccg gtggcgacga ttttactgtc
    20761 ggtcgccatt accctgtgcg gcatactggg tttccgtatg ctgccggtcg ccccgctgcc
    20821 gcaggtcgat tttccggtga ttatggtcag cgcctcgctg cccggtgcgt cgccggagac
    20881 aatggcgtct tccgttgcca cgccgctgga gcgctcactt gggcgcattg ccggggtcag
    20941 tgaaatgacc tccagcagtt cgctcggcag cacgcgtatt attttgcagt ttgattttga
    21001 ccgggatatc aacggtgcgg cgcgtgatgt gcaggcggcg atcaacgccg cacaaagtct
    21061 gctgcccagt gggatgccca gccgcccgac ttatcgcaaa gcgaacccgt cggatgcgcc
    21121 aattatgatc ctcacgctaa cctccgatac ttattcgcag ggtgaactgt acgatttcgc
    21181 ctcgacgcag ctggctccga cgatttcgca aatcgacggt gtcggtgatg tcgatgtcgg
    21241 cggcagctcg ctgcccgccg tgcgcgtcgg gctgaacccg caggcgctgt ttaatcaggg
    21301 cgtgtcgctg gacgacgtac gcactgccat cagcaatgcc aacgtgcgta aaccgcaggg
    21361 ggcgctggaa gatggcactc accgctggca gatccagacc aacgatgaac tgaaaaccgc
    21421 cgctgaatat cagccgctga ttattcacta caacaacggc ggcgcggttc gtctgggcga
    21481 tgtggcgacg gtgaccgact cagtgcagga tgtgcgcaac gccgggatga ccaacgccaa
    21541 accggctatt ttactgatga tccgcaaatt gccggaagcc aatattatcc agacggtaga
    21601 cagcatccgg gcaaaattac cggagttgca ggaaaccatt ccggcggcga ttgatctgca
    21661 aattgcccag gatcgctccc ccaccattcg cgcctcgctg gaagaagtcg agcaaacgct
    21721 gattatctcg gtggcgctgg tgattctggt ggtcttttta ttcctgcgct cgggtcgcgc
    21781 cactattatt cccgccgttg cggtgccggt ttcgctgatt ggtacgtttg cggcgatgta
    21841 cctgtgcgga ttcagtctca ataacctttc gttaatggcg ctcaccatcg ctactggttt
    21901 cgtggtggat gacgccatcg tggtgctgga aaacattgca cgtcatctgg aagcgggaat
    21961 gaaaccgttg caagccgcac tgcaaggtac tcgcgaagtc ggttttacgg tgctgtcgat
    22021 gagtctgtcg ctggtggcgg tgttcctgcc gctgctgttg atgggcggat tgccgggccg
    22081 actgttacgc gagtttgccg tgacgctttc tgtcgccatt ggtatttcgt tgctggtttc
    22141 tctgacatta acgccaatga tgtgtggctg gatgctgaaa gccagcaagc cgcgcgagca
    22201 aaagcgactg cgtggttttg gtcgcatgtt ggtagccctg caacaaggct atggtaagtc
    22261 actgaaatgg gtgctcaatc atacccgtct ggtgggcatg gtgctgcttg gcaccattgc
    22321 tttgaatatc tggctatata tctcgattcc gaaaaccttc ttcccggagc aggacactgg
    22381 cgtgttgatg ggcgggatac aggcggatca gagtatttcg tttcaggcga tgcgcggtaa
    22441 gttgcaggat ttcatgaaaa ttatccgtga cgatccggca gtggataatg tcaccggctt
    22501 taccggcggt tcgcgagtga acagcgggat gatgtttatc accctcaagc cacgcgacga
    22561 acgcagcgaa acagcgcagc aaattattga ccgtctacgc gtgaagctgg cgaaagaacc
    22621 gggggcgaat ctgttcctga tggcggtaca ggatattcgc gttggcggac gtcagtcgaa
    22681 cgccagctac cagtacacgt tgttatccga cgacctggcg gcactgcgcg aatgggagcc
    22741 gaaaatccgc aaaaaactgg cgacattgcc ggaactggcg gacgtgaact ccgatcagca
    22801 ggataacggc gcggagatga atctggttta cgatcgcgac accatggcac ggctgggaat
    22861 cgacgtacag gccgccaaca gcctgttaaa taacgccttc ggtcagcggc aaatctcgac
    22921 catttaccag ccgatgaacc agtataaagt ggtgatggaa gtggatccgc gctataccca
    22981 ggacatcagc gcgctggaaa aaatgttcgt aatcaataac gaaggaaaag cgatcccgct
    23041 gtcgtatttc gctaaatggc aaccggcgaa tgccccacta tcggtgaatc atcagggatt
    23101 atcggcagcc tcgaccatct cgttcaacct gccgaccgga aaatcgctct cggacgccag
    23161 tgcggcgatc gatcgcgcaa tgacccagct tggtgtgcct tcgacggtgc gcggcagttt
    23221 tgccggcacg gcgcaggtgt tccaggagac gatgaactca caggtgatcc tgattatcgc
    23281 cgccatcgcc acggtgtata tcgtgctggg tatcctttac gagagttacg tacatccgct
    23341 gacgattctc tccaccctgc cctcggcggg cgttggagcg ctgctggcgc tggagctgtt
    23401 caatgccccg ttcagcctaa tcgccctgat agggatcatg ctattaatcg gcattgtgaa
    23461 gaaaaacgcc attatgatgg tcgattttgc gcttgaagcc caacggcacg gtaacctgac
    23521 gccgcaggaa gctattttcc aggcctgtct gctgcgtttt cgcccgatta tgatgactac
    23581 cctggcggcg ctgtttggcg cgctgccgct ggtgttgtcg ggcggtgacg gctcggagct
    23641 gcggcaaccc ttggggatca ccattgtcgg cggtctggtg atgagtcagc tcctgacgct
    23701 gtataccacg ccggtggtat atctcttttt cgaccgtctg cggctgcgtt tttcgcgtaa
    23761 atctaaacaa acggtaaccg agtaaatgac agatcttccc gacagcaccc gctggcaatt
    23821 gtggattgtg gctttcggct tctttatgca gtcgctggac accaccatcg taaacaccgc
    23881 ccttccctca atggcgcaaa gcctcgggga aagtccgttg catatgcaca tggtcattgt
    23941 ctcttatgtg ctgaccgtgg cggtgatgct gcccgccagc ggctggctgg cggacaaagt
    24001 cggcgtgcgc aatattttct ttaccgctat cgtgctgttt actctcggtt cactgttttg
    24061 cgcgctttcc ggcacgctga acgaactgtt gctggcacgc gcgttacagg gcgttggcgg
    24121 tgcgatgatg gtgccggtcg gcaggttgac ggtgatgaaa atcgtaccgc gcgagcaata
    24181 tatggcggcg atgacctttg tcacgttacc cggtcaggtc ggtccgctgc tcggtccggc
    24241 gctcggcggt ctgctggtgg agtacgcgtc gtggcactgg atctttttga tcaacattcc
    24301 ggtggggatt atcggtgcga tcgccacatt gatgttaatg ccgaactaca ccatgcagac
    24361 gcggcgcttt gatctttccg gctttttatt gctggcggtt ggcatggcgg tattaaccct
    24421 ggcgctggac ggcagtaaag gtacaggttt atcgccgctg gcgattactg gcctggtcgc
    24481 agttggtgtg gtggcactgg tgctttatct gctgcacgcc agaaataaca accgcgctct
    24541 tttcagtctg aaactgttcc gcactcgtac cttttcgctg ggcctggcgg ggagctttgc
    24601 cggacgtatc ggtagcggca tgttgccctt tatgacgccg gttttcctgc aaattggcct
    24661 tggcttctca ccgtttcatg ccggactgat gatgatcccg atggtgcttg gcagcatggg
    24721 gatgaagcgg attgttgtac aggtggtgaa tcgctttggt tatcgtcggg tactggtagc
    24781 gaccacgctg ggtctgtcgc tggtcaccct gttgtttatg actaccgctc tgttgggctg
    24841 gtactacgtt ttgccgttcg tcctgttttt acaagggatg gtcaactcga cccgtttctc
    24901 ctccatgaac accctgacgc tgaaagatct cccggacaat ctggcgagca gcggcaacag
    24961 tctgctgtcg atgattatgc aattgtcgat gagtatcggc gtcaccatcg ccgggctgtt
    25021 gctgggactt tttggttcgc agcatgtcag cgtcgacagc agcaccacac aaaccgtctt
    25081 tatgtacacc tggcttagca tggcgtttat catcgccctt ccggcgttca tctttgccag
    25141 agtgccgaac gatacgcatc aaaatgtagc tatttcgcgg cgaaaaagga gcgcgcaatg
    25201 aagttctggc gacctggtat taccggcaaa ctgtttctgg cgattttcgc cacctgcatt
    25261 gtcttgctga tcagtatgca ctgggcggtg cgtatcagtt ttgagcgtgg ctttattgat
    25321 tacatcaagc atggtaatga acagcgatta caactgttaa gtgatgcgct tggcgagcag
    25381 tatgcgcagc atggcaactg gcgcttcctg cgcaacaatg atcgctttgt ctttcagatc
    25441 ctgcgttcat ttgaacacga taattcggaa gataaacccg gcccgggtat gccaccacac
    25501 ggctggcgta ctcagttctg ggtggttgat caaaacaaca aggtgctggt tggtccgcga
    25561 gcgccgattc cacctgacgg tacacggcga cccattctgg tcaacggtgc ggaagtgggc
    25621 gcggtgattg cctcccctgt tgagcggctg acccgcaata ctgatatcaa tttcgataaa
    25681 caacagcggc aaaccagctg gctgattgtc gccctggcaa cgttactcgc ggcactcgct
    25741 acttttctgc tggcgcgcgg tttgctggca ccggtaaaac gacttgtcga tggcacgcac
    25801 aaactggcgg cgggcgattt cactacccgc gtaacgccca ccagtgaaga tgaactgggc
    25861 aaactggcgc aagacttcaa ccagctcgcc agcacgctgg agaaaaacca gcaaatgcgg
    25921 cgcgatttta tggccgatat ttctcacgaa ctgcgtacgc cattagcggt gctgcgcggt
    25981 gaactggaag ccattcagga tggcgtgcgc aaattcacgc cggagacggt ggcgtcttta
    26041 caggcggagg tcggtacact gaccaaactg gttgacgatc ttcatcagtt gtcgatgtct
    26101 gatgaaggcg ctctcgccta tcaaaaagca ccggtagatt tgatcccact gctggaagtg
    26161 gcaggcggcg catttcgcga acgatttgcc agccgtggcc tgaaactgca attttccctg
    26221 ccagacagta tcaccgtatt tggcgatcgc gaccgtttaa tgcagttatt caataactta
    26281 ctggaaaaca gcctgcgcta cactgacagc ggcggcagcc tgaaaatctc tgccgagcag
    26341 cacgacaaaa cggtgcgcct gacctttgcc gacagtgcgc caggtgtcag tgacgatcag
    26401 ctacaaaaat tgtttgaacg tttttatcgc accgaaggtt cccgcaaccg cgccagcggc
    26461 ggttccgggc tggggctggc gatttgcctg aacattgttg aagcacataa tggccgcatt
    26521 attgccgccc attcgccttt tggcggggta agcattacag tagagttacc gctggaacgg
    26581 gatttacaga gagaagtatg accgagttac caatcgacga aaacacaccg cgtattttga
    26641 tcgtggaaga tgaaccgaag ctgggacagt tgctcattga ttatctgcgt gctgcgagct
    26701 atgcgccgac gcttatcagc cacggcgatc aggtactgtc gtacgtgcgc cagacgccgc
    26761 cggatctgat cctgttggat ctgatgctcc ctggcaccga tggcctgacg ctgtgccggg
    26821 aaattcgtcg tttttctgac attcctatcg tgatggtgac agcaaaaatc gaagagatcg
    26881 accgcctgct agggctggag attggcgcag atgactacat ttgcaagccg tacagcccac
    26941 gggaagtggt agcgcgtgtc aaaaccattt tgcgccgttg caaaccgcag cgtgagttgc
    27001 agcaacagga tgctgaaagc ccgttgatta tcgacgaagg tcgttttcag gcttcatggc
    27061 gcggtaaaat gcttgacctg acgcctgcgg aatttcgtct gctgaaaacg ctctctcatg
    27121 aaccaggaaa agtgttctcc cgcgagcaat tgctcaatca tctttatgac gactaccgcg
    27181 tagtaaccga ccgtaccatc gacagccaca ttaaaaacct gcgccgcaag ctggaatctc
    27241 tcgacgccga acagtcattt atccgcgccg tttacggcgt cggttaccgc tgggaagccg
    27301 acgcctgccg catcgtttag ttttagcgac attactttgt tagccggaga tggcattcag
    27361 accagatccg gcaacattat cccacgcatg gtcagcaaac tgacattatc tctccgccct
    27421 ggcaggcatc agaaaatcgc tcatacttta atcggtaaac agcaccttta gatgctgttt
    27481 tcgatacgca atttcaatca aggagtcatt atggctggtt ggtttgaact cagtaagagc
    27541 agtgatagtc agttccggtt tgtgctaaaa gcgggcaatg gtgagacgat cctcaccagc
    27601 gagctgtata cctcaaaagc ctctgcggaa aagggcatcg cgtcggtgcg tagcaacagc
    27661 ccgcaagaag aacgctatga gaaaaaaacg gcaagcaacg gcaaattcta tttcaatctg
    27721 aaagccgcca atcatcaaat tatcggctcc agccagatgt atgccaccgc gcaatctcgt
    27781 gaaaccggaa ttgcctccgt taaagccaat ggcacaagcc agacggtgaa agacaatacg
    27841 taattacgat gccgggcgtg ggttgttggc gtccggcgct ttaccagatc ccccctacag
    27901 cgctacaatg cccgccctta aagtgggggc actcccctaa ccgcttcatc aggtgaagcg
    27961 gatctgacct gtcatcagaa cgagagaatt atgtttaaac cggaactcct ttccccggcg
    28021 ggaacgctga aaaatatgcg ttacgctttc gcttatggcg cagatgctgt ttatgcgggc
    28081 cagccgcgtt actccctgcg tgtgcgcaac aacgaattca accacgaaaa ccttcagctc
    28141 ggcatcaatg aagcccacgc gctggggaaa aagttttatg tcgtggtcaa cattgcaccg
    28201 cacaacgcca agctgaaaac ctttatccgt gacctgaaac cggtggtgga aatggggccg
    28261 gatgcgctga ttatgtccga tccagggctg attatgctgg tgcgtgagca cttccctgaa
    28321 atgccgatcc atctctcggt acaggctaac gccgtgaact gggcgacggt gaaattctgg
    28381 cagcaaatgg gactgacccg cgtgatcctc tctcgcgagc tgtcgctgga agagattgaa
    28441 gagatccgca atcaggtgcc ggatatggag atcgagatct tcgttcacgg cgcactgtgc
    28501 atggcctact ccggtcgctg cctgctctct ggctatatca acaagcgcga tccgaaccag
    28561 ggtacctgca ccaacgcctg ccgctgggag tacaacgtcc aggaagggaa agaagatgac
    28621 gttggcaaca tcgtacacaa gtacgagccg attccggtgc aaaatgttga gccgacgctg
    28681 ggtatcggcg cgccaaccga caaagtgttt atgatcgaag aagcccagcg tccgggcgag
    28741 tatatgaccg cgtttgaaga tgagcacggc acttacatca tgaactcgaa agatctgcgc
    28801 gccatcgccc atgtagaacg cctgaccaaa atgggcgtgc attcgctgaa aatcgaaggc
    28861 cgtactaaat ctttctacta ttgcgcacgc accgcacagg tttatcgtaa agctatcgat
    28921 gatgccgctg cgggcaaacc gttcgatacc agcctgctgg aaactctgga aggtctggcg
    28981 catcgtggct ataccgaagg tttcctgcgt cgtcatactc acgacgatta tcagaactac
    29041 gaatacggtt attcagtttc tgaccgccag cagtttgttg gtgagtttac cggtgagcgc
    29101 aagggggacc tcgcggcggt agcggtgaaa aataaattct ccgttggcga cagccttgag
    29161 ctgatgacgc cgcaaggcaa cattaacttt acccttgagc acatggaaaa cgccaaaggc
    29221 gaagctatgc cggtagcacc aggcgatggt tatactgtgt ggctcccggt gccgcaggat
    29281 cttgagctaa attacgcgct gctgatgcgt aatttctccg gggaaactac gcgtaacccc
    29341 cacggtaagt gattaatttc gattattttt cccggatgga aaattcttag aaaccgatca
    29401 catacagctg catttattaa ggttatcatc cgtttcgctg aaaaacataa cccataaaat
    29461 gctagctgta ccaggaacca cctccttagc ctgtgtaatc tcccttacac gggcttattt
    29521 tttacgcgta atacaatgaa ataaaaggat ttatttctgg tcacgtccac atattgacca
    29581 catcgacaaa aaaagcccct caactgaggg gctttctgtt tgtaattaca tccacataat
    29641 ttgctgccct gacggcaacg ggtgcggtct tacggcgtgg acttctcccg gcttcacgat
    29701 gtatcgctgt accgactcat aagtgatgaa cgtggcgctg caattcacgt tctgacactg
    29761 gtgataacgc tcttttgtcg tgtcagtgat atagcgactt gtacgcgcat gtgcggcatg
    29821 ctggcataaa ggacaatgaa acatcgcgag cacctcttcc ggttttgttg atggtgccat
    29881 tttagttaat ttatccttat aaaacaaaca gataaaataa aaacatcatt catcatcttc
    29941 tgtttcgtat tccacatcag aaagcctgac ctcaagctct aaggacgtcg tgaagccgct
    30001 attattcaga aaatgtgtca ccttagtgat ggtccagtcc tgctcgtcta tgacgcgctt
    30061 aaagcctgac actctgaccg gtgtttccgt gtaaatatct gcacgaccgg tagccaggat
    30121 gatggagaac tccgcaacgc cccgttgcag tttgtcccac ttcgcctgag cagcacgcat
    30181 ggcctgtgct ttcgtggcat ataccgtggt cagggcaaaa acgttgtcag cctcaccggc
    30241 catgtattca ccttcgcgcg cttccggtac ttttggcgct ttcttctgcc tgaccggttt
    30301 cgctttcggg tgctccagtg cgcgcaggtg tttctctttc tttttgcgtt tcagttttac
    30361 cttctgcttt tgcggtttcg ggtctttggt gtgtaaccac tttgccgtta caccggtata
    30421 ggctccacgg tcagcaatcg caaaatgatg gcggtcgccg tcgctgcggg ttatggtgac
    30481 ctgcgggatt tttttaccgc tggccgtcac cccctgcccc gctttgagaa acaacagttt
    30541 tcccattttt accgagacct caccgccgtt gcgttcagca aggcgggtca ggaattttgc
    30601 atcagactcc tgcgactggt cgatgtgcgg gattttaatt ccggccagtg acggcgcgac
    30661 actggcttcc agcctgttac gggaggctat tgcctcaaca atcgcaccga gcgtggtgtc
    30721 atgccaggag tcttcacggc gggaattgag cgtcccgcga aaatctgcac tccgggcgcg
    30781 gatggtgacc acatccggcg cgccccggtg ttcaacctca tcaacggtaa atttcccttt
    30841 gcataccagg gcaaaacctt tccagccgat atacaccgtc aggacagcgc cacgaatcgg
    30901 cagcccgacc tgcccgtcgg catcgttcag ttcaatatca agctggtcag cctcaaagcc
    30961 ccggttatcc gtcagagtca tgctcatcag acggtcgctg atattgccgg taatatccct
    31021 gctgtcgagc atcagcatgt aatccggcgt cagcgtactg cctgcatcaa atgtcagtgc
    31081 atccagcatt atcccgcccc cgtcatacct gtgaatttag tcgccatact gccagcctta
    31141 ccgatgagcg attccgcctg tttaccgata tcgccataaa gcgcggccag tggttcgtca
    31201 acgcgggtga gcgacagcgt aaaatcaatt tttcggggtg tgccgtctgc aaagaaaata
    31261 ctccctgttt cactcaccct gctgatgaca tacatgccgt aaatcatgcc ggtgccatcc
    31321 agcaacggcc acgcccgacc ttcctctgcc atcagcctga gcgtggtcat cgtcagcttg
    31381 ccgccggtca gttcgggata aagcacaccg gccagcgtca tgttttcctc gccaacgccg
    31441 agaaactggt aggcatcccg tttaccgata cgggaatttg acggccagcg ataatctgat
    31501 tcacgctgca tggtctggtg tggcagcgtc tggcgcataa aaacaaacat acctaacgcg
    31561 agcatcattt ttcgtcacct ccttaaccgt catgcatcat gctggcacgg gcgcgcgcac
    31621 gtttatcccg ctcgtatttt tcgagcgcat cctgtaactg gcggtcaagc tgtgtccccg
    31681 gcgcagtacc acccgtcagg ctgatgtgat attcgttttt actctggtcc acataagagc
    31741 ggccagccgg tgccgtgacc ggctgataag cctgatagcc tgcataagag ctggtcgccg
    31801 gaatataacc accggtgcca tacgtggcgg catgagttct ggcggcggtc tggtcaagtg
    31861 tgtctgactc tttgttgata acaccgagtt tttccagtac ccagtcaata ccactgcgca
    31921 gtttgttgaa cgcattaagc ggcagcatca acgcgtcagc cagtgcctgc ccgaacatga
    31981 cgcccgtgtc acggcaacgg ttcagggtgt cctgggtggc tttgaccggg gcaatcagtt
    32041 ttttaaacca ctgccacgcg gcctgtaact tttcacccag ccagtcaaac agcggcttaa
    32101 gtggcgtgaa cagttccccc accggcgcaa atgcagcttt cagcccttca accacaccgc
    32161 caaagaatgc gctgacaggc tcccagtatt tacggataag caacgccccg gcgacaattg
    32221 cagccaccac ggccacaacc ggccagctaa tcgccccgat ggccgtcata acagcactgc
    32281 caaccgtcgt gaagattgcc cccattgcgc ctgctgccgc gatgatggca ttaatgccgg
    32341 tgataaccgg ccaggctaca agaccaattg caccgatgat gccaataagc gccagtgcgc
    32401 caccgacaat aaggccgatg gttgacgcca gtgatttgtt tttctggatc cagccgtcga
    32461 gttttaacac atactttgtg gccgtctgcg tgagattacg cagtgcgcct tcctgttggt
    32521 caaacaggtc agtccccacc gcctcataag cggactgaaa ctccttaaag tcaccgccga
    32581 ggttgtcctg catgatattt accagctctg cggtcttccc gtctgaggct ttaaacgcag
    32641 cggtcagttt gtccagcttt ccggttgagg cggcagtcat cagcacggcg gcggctgaac
    32701 tggcctcctc cccgaaaatg gttttcatgt attcagcctg ctgggcagta ccgagccggt
    32761 ttttctcaaa actggcctgc atttctttca gaatggtaaa tattggtcgg gtattcccct
    32821 tgctgtctga ggttttcact ccaagctctt tgagtgcatc ccatgctttt cccgtcggtg
    32881 cctgcaggcg acttaacacg gcacggcttc ccgtccccgc cattgagcct gtgatttttg
    32941 catcatgcag cgccccgacc attgcggcgg tttcttcaat gctgacaccg gcattttttg
    33001 ccacaggtgc ggcataggtc agcgcatcgc tcataccgtc aaaatcggcg gcggttttgt
    33061 tcatcgtcat ggagagaaca tccccgatat gagcaacctt atcgtttgaa agctgaaagg
    33121 cagatttcat ccccatcagc agggcggcgt tttcttccat cgtgcgacgg ttcgccagcg
    33181 ccatattcag cgtgaccggc gttgccgcct gaatggcatc aacatcccca cccgctttcg
    33241 cgatgataat ctgtgcaccg gccgcatcat ccgccgaggc ggcggtattg tcgccgagct
    33301 ggcgcgcctg cttgcgtagc gcggccattt cggcggagtc ttttgccaca ccgagcacgg
    33361 cctgtaattc tgagtttttc tgcgcaaatt cataaccggg catcagcagc ttaacaccgg
    33421 ccatcgttcc cgccgccgca atccccacac cggcagcgcc cactgaggcc atatttccgg
    33481 ccagctcctt tcctgcctga tagcgctgtt ttactgcgtt aagttttgcc tgttgcgcac
    33541 tgacacgcgc cagcgcgtca cgctgccggt taagctgtgc ggtggtttca ctgatacggt
    33601 ttttcagccc cagctcatca tgtgcaagat tgcgggtatt aattcccaca gcggccagtt
    33661 cccgctgctg gcgtttaacg gaatccgtca ggcggttata tttcgcctgt aagtcctccg
    33721 ccgcacgctt tgcggattcc agcactttcg cctgagcacg ggtcggacgt tcggtgtttt
    33781 taaactgtgt ggcaagggct tcggcctcct gccgtgcctt ttcaagtgca tgaccagtca
    33841 cggcgagctg tgcgctggtc ttgcggaatc cctcaatacg ggatgcctgc ccgttcagct
    33901 cgcgcagtga tttttgtgtt tcccggatat cccccgacag cgacttactc gctgtgcgga
    33961 tggatttaaa cgggcgggat gcctggtcaa cagccctgag caatacctgt aattttacat
    34021 tgttactcat tcgtgtttcc gcttcgcctg agcgcctttt cgcgccatgt gatgagttcg
    34081 gtcaggctca tgggatacag ttctgatggc ggccagtgaa ataccactgc cacatccgcc
    34141 atcaggtcat cgaccgagag atttttcgga aacgtcactg caccgagttc ggcgacaaaa
    34201 aaccgaccac cttaccggcc agcgccacaa ggtcaggcag ttccagcgcg gcgacctcct
    34261 gttcggtcag catcggtgcc gtcatgcgcg gcagcacttt aatcagtgca tcgacttcgg
    34321 agttcgcgac cgcagccaga ctgacaccgc gcagcgtccc ggcattgggt ttcatcagcg
    34381 tgacctgttc gataacctgc tcaccacgct tgaccggatt gtccagggta atcacatttt
    34441 ctttgttcat ggttttctca cttctgaatc agggttaacc ggtcagcctg gctgaccgga
    34501 tgaaaatcac aggccgatat tgcggcggtg ttgctccagc cggtcgacgc cgttcacctt
    34561 ctcaatcatg ttgatggtgt cgatttcgac cagctcctta ccgtccatcg tcagccggaa
    34621 ataggtgcag accacggaga ttttcgactc ggtgtcttct ccctgtttac cctcgccggt
    34681 gtcgatttct ttctgacggc cacgcatgac cacctcgacg gccaccgttt cgccggtatc
    34741 gtcgcgctgg taagagcctg caaaacgaat cggtacggca tccacaccgg ttgcggcgta
    34801 aagctcccag ataaccgaat ccgggaagcc accgagcgac cactccattg acagcgcatc
    34861 gtcatcaagg ccgaggtcta ccggtgcgct gccattcatc cccgcaccgc gatagttttc
    34921 gagcttacgg gtcagttttg gcagcgtgac ggacttcgcg acgccctgat agctgtagcc
    34981 gttcagaaag acgttcatta acttgagttt gcgcggcatt gccatcggtc aggctcctta
    35041 attgctgtta accgaggtga ccagatttgc caggtattta tcggtgatac gctggcgcag
    35101 ggtcaggttt tcaagaggag gtaccggtgt atagtcgtag tcgatataca gttttccggc
    35161 cttgagggtt tccgcatcgc tggattcttc gctgaaccag caggtcgcat ccacgatata
    35221 gccgtttgtt ttcagctcac ggaatttggc attgatgccg tcaacgatgt cgcgaatcag
    35281 cgttgcggtg atgggcttgt ccaccgccca catgtgcgcc tcagccatcg tgtcggccag
    35341 cacctgcgcg gtgcgggtgt agttttcaaa gaggaacagc gggtcatcag agcaggtacg
    35401 gttaccccag aagcggaaac cgtcgcggcg aatcagcgta gtgacgcctg actcgttaag
    35461 caggtcagca tcggtgccgg actcctgcaa atcccagaat acagatgcgc tgatgccgtt
    35521 aacaccgttt accccgacgt tggacagcgt tttatgccag ccctgctcct ggtcgatttt
    35581 agcgcgcaga cccagcgcac gggcggtggc atacgcggtg gcggtggtgc tgctgaccgt
    35641 atcccatgcg aggaaatccg gccagatgac catcagctca cgctggctga aattctggcg
    35701 gtaggctttc acctcggaaa tggttttaca gccccatgcg ctgatatacc cgaaagcgcg
    35761 cagcttctga cagactgatg ccagtgcaac agccacctct ttggtgtcca gtcccggcac
    35821 gccgagaata cgcggtttaa caccggttac cgactctgcc gccagcaggg ctttcagtcc
    35881 ggtatactga ccgttttcgt cagtggtgcc gatgatattg gaaacggtct gcgcgagttt
    35941 cgtttcttcg tcgtcgccgg tgccgtcttc aacacgcacg acaacggtaa ccggttttga
    36001 ctggtcagcg atggcctgca acgatgccgc cagcgtgcct tttttaccgg cctttgcaat
    36061 tgcgctctgc acattggtaa tcagcacagg tttattgagg gggaaggttt ccgcatccgc
    36121 atcgctggcc gtgcagacca tgccgacaat ggcggtggat acggtggaaa tgacgcgggt
    36181 gccgtcgtta atctccagca cctgcacgcc gtgatgataa tcactcatcc gtttaactcc
    36241 gtggttaatg ggtgagtgat attttcagtt gtgccggaga tgtcaggcta tttgtcccgg
    36301 ttggctaagg gatgacacaa tttattcttt gtcgctgatg aggggaattt tttatagagc
    36361 gtggacaggc caatatcaaa aatcagcgcc acacgttgac gagattcccc cgcagccagc
    36421 aaccgtccga cctgctccca ctcactcgtg gtgagtttcg gtcgtctgcc accaatacga
    36481 cctttggctc tggccgcttc cagtccggcg cgtgtccgct cgacaatgag ttctcgctcc
    36541 atttctgcca gggcccccat cacatgaaag aaaaagcgcc ccatcggcgt gctggtatca
    36601 atagcatccg tcaggctgcg aaaattaacg ccgcgttcgc gcagttcctc aaccagaatg
    36661 accagatgcc gcatactacg ccccagccta tccagcttcc agactaccag agtgtcacct
    36721 gctgataatg ttctgagcag ttttttcagt cccggtcggt cggacttcgt gccgctgatt
    36781 ttatcctcaa aaatcagctc acatcctgca cactccagcg cattacgctg caattccgtg
    36841 ttctggtcat ttgttgacac gcgtacatag ccaataagca tgagcatctc cctgaataaa
    36901 aaccggagat gatgccagtt acccgttatt tctgcatttt ca
//
LOCUS       DBFIFI010000025        36488 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.025, whole genome shotgun sequence.
ACCESSION   DBFIFI010000025 DBFIFI010000000
VERSION     DBFIFI010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36488
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.025"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(85..219)
                     /locus_tag="N7B39_001232"
     CDS             complement(85..219)
                     /locus_tag="N7B39_001232"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2421247.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            295..1185
                     /gene="metF"
                     /locus_tag="N7B39_001233"
     CDS             295..1185
                     /gene="metF"
                     /locus_tag="N7B39_001233"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HGU2421248.1"
                     /translation="MNFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            1514..3694
                     /gene="katG"
                     /locus_tag="N7B39_001234"
     CDS             1514..3694
                     /gene="katG"
                     /locus_tag="N7B39_001234"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HGU2421249.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            3788..4693
                     /gene="yijE"
                     /locus_tag="N7B39_001235"
     CDS             3788..4693
                     /gene="yijE"
                     /locus_tag="N7B39_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HGU2421250.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAAVFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKRI"
     gene            complement(4720..5337)
                     /gene="yijF"
                     /locus_tag="N7B39_001236"
     CDS             complement(4720..5337)
                     /gene="yijF"
                     /locus_tag="N7B39_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HGU2421251.1"
                     /translation="MKASLALVSLLTAFTSYSLKSPAIPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYVQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(5611..6714)
                     /gene="gldA"
                     /locus_tag="N7B39_001237"
     CDS             complement(5611..6714)
                     /gene="gldA"
                     /locus_tag="N7B39_001237"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HGU2421252.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENSPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(6725..7387)
                     /gene="fsa"
                     /locus_tag="N7B39_001238"
     CDS             complement(6725..7387)
                     /gene="fsa"
                     /locus_tag="N7B39_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HGU2421253.1"
                     /translation="MELYLDTANVAEVERLAHIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(7399..9900)
                     /gene="ptsP"
                     /locus_tag="N7B39_001239"
     CDS             complement(7399..9900)
                     /gene="ptsP"
                     /locus_tag="N7B39_001239"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HGU2421254.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFSRSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            10209..11288
                     /gene="frwC"
                     /locus_tag="N7B39_001240"
     CDS             10209..11288
                     /gene="frwC"
                     /locus_tag="N7B39_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HGU2421255.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            11303..11623
                     /gene="frwB"
                     /locus_tag="N7B39_001241"
     CDS             11303..11623
                     /gene="frwB"
                     /locus_tag="N7B39_001241"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HGU2421256.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            11674..13971
                     /gene="pflD"
                     /locus_tag="N7B39_001242"
     CDS             11674..13971
                     /gene="pflD"
                     /locus_tag="N7B39_001242"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HGU2421257.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELGQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAASCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFDQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVGNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            13937..14815
                     /gene="pflC"
                     /locus_tag="N7B39_001243"
     CDS             13937..14815
                     /gene="pflC"
                     /locus_tag="N7B39_001243"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312908.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HGU2421258.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            14817..15158
                     /gene="frwD"
                     /locus_tag="N7B39_001244"
     CDS             14817..15158
                     /gene="frwD"
                     /locus_tag="N7B39_001244"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HGU2421259.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCLLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQQVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(15145..15996)
                     /gene="yijO"
                     /locus_tag="N7B39_001245"
     CDS             complement(15145..15996)
                     /gene="yijO"
                     /locus_tag="N7B39_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU2421260.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(16211..17944)
                     /gene="eptC"
                     /locus_tag="N7B39_001246"
     CDS             complement(16211..17944)
                     /gene="eptC"
                     /locus_tag="N7B39_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HGU2421261.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNMFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELNALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQEVLKDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLNAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKENTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(18127..20778)
                     /gene="ppc"
                     /locus_tag="N7B39_001247"
     CDS             complement(18127..20778)
                     /gene="ppc"
                     /locus_tag="N7B39_001247"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HGU2421262.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKTLWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(21130..22281)
                     /gene="argE"
                     /locus_tag="N7B39_001248"
     CDS             complement(21130..22281)
                     /gene="argE"
                     /locus_tag="N7B39_001248"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HGU2421263.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASTGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICAWCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            22435..23439
                     /gene="argC"
                     /locus_tag="N7B39_001249"
     CDS             22435..23439
                     /gene="argC"
                     /locus_tag="N7B39_001249"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HGU2421264.1"
                     /translation="MLNTLIVGASGYAGAELVTCVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLDAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKPGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            23447..24223
                     /gene="argB"
                     /locus_tag="N7B39_001250"
     CDS             23447..24223
                     /gene="argB"
                     /locus_tag="N7B39_001250"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HGU2421265.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            24284..25657
                     /gene="argH"
                     /locus_tag="N7B39_001251"
     CDS             24284..25657
                     /gene="argH"
                     /locus_tag="N7B39_001251"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HGU2421266.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEELPLTELQKFSPVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FARARLG"
     gene            25924..26841
                     /gene="oxyR"
                     /locus_tag="N7B39_001252"
     CDS             25924..26841
                     /gene="oxyR"
                     /locus_tag="N7B39_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HGU2421267.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(26824..28224)
                     /gene="sthA"
                     /locus_tag="N7B39_001253"
     CDS             complement(26824..28224)
                     /gene="sthA"
                     /locus_tag="N7B39_001253"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HGU2421268.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            28554..29720
                     /locus_tag="N7B39_001254"
     CDS             28554..29720
                     /locus_tag="N7B39_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HGU2421269.1"
                     /translation="MIEHYIRGFEEELREIRHQIHENPELGLQEFKTSALVAEKLRQW
                     GYEVEQGLATTGIVATLKVGDGEKSIGLRADMDALPIYENSGKPWASKHPGLMHACGH
                     DGHTTILLGAARYFAETRRFNGTLRLIFQPAEEMINGGEIMVKEGLFDHFPCDVIFGM
                     HNMPGLPVGKFYFQPGALMASMDQFHITVRGCGGHGAIPHKAIDPVLVAAHITTALQS
                     IVSRNVDPLEAAVITVGSIVAGEAANVIPDSAEMKISVRSLSRDTRQLLLTRIPALAQ
                     AQAASFGATAEVTHVNGTPVLVNDEEMARFAWQVACKTFGEDRAEFGIKPLMGSEDFS
                     FMLEAQPKGGFLLFGNGDVGEGSCMVHNPGYDFNDASLVPASSYWGALVEAWLQ"
     gene            29763..31079
                     /locus_tag="N7B39_001255"
     CDS             29763..31079
                     /locus_tag="N7B39_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate-proton symporter"
                     /protein_id="HGU2421270.1"
                     /translation="MTAIVNETVSVKKKPSGRRVIFASAFGNALEFFDFGVYNFFVVY
                     ISTLFFPPSADKNVALLLAFATFGVSFFMRPLGGIIVGAWADRFGRKPAMVFTIALMS
                     LGTLMIGIAPTYETAGYWGTATLVLARLIQGVAAGGEVGASMSLLVESAPANRRGFYS
                     SWSLATQGLATTFGGVVALGLSAWLPFATGSETVMAEWGWRVPFFIGVLLAPVGCWLR
                     LSLENDVPEPAHNKKAAASESAFSLLMQHKATIANGILLAIGSTVATYISLFYYGTWA
                     AKYLGMNQNYSHAAMLLAGVITFVGALLVGMLCDSVGRKKLILISRVMVLICSWPSFW
                     LLVNYPSPGMLLTVVFVMVSFTTLGGVPVMLLISELLPKRIRALGFALVYSIGVAIFG
                     GFAQYFATQSIVLLDSLTAPAWYLGGGTLLSMLALLYVKEPAKELQ"
     gene            31183..31833
                     /gene="fabR"
                     /locus_tag="N7B39_001256"
     CDS             31183..31833
                     /gene="fabR"
                     /locus_tag="N7B39_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HGU2421271.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            31833..32192
                     /gene="yijD"
                     /locus_tag="N7B39_001257"
     CDS             31833..32192
                     /gene="yijD"
                     /locus_tag="N7B39_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HGU2421272.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(32232..33332)
                     /gene="trmA"
                     /locus_tag="N7B39_001258"
     CDS             complement(32232..33332)
                     /gene="trmA"
                     /locus_tag="N7B39_001258"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HGU2421273.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECSVLLTAK"
     regulatory      33446..33636
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            33701..35545
                     /gene="btuB"
                     /locus_tag="N7B39_001259"
     CDS             33701..35545
                     /gene="btuB"
                     /locus_tag="N7B39_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HGU2421274.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGGSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQPDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLVDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNIIIGHGNVGAG
                     VDWQKQSTAPGTAYVKDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            35490..36347
                     /gene="murI"
                     /locus_tag="N7B39_001260"
     CDS             35490..36347
                     /gene="murI"
                     /locus_tag="N7B39_001260"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HGU2421275.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         8302 a         9055 c        10095 g         9036 t
ORIGIN      
        1 tgaaatatat tgaattatca taggattagg ccggatgaag cgtttacgac gaatccggca
       61 agaagcaata agtacatggt tagtttatat ttgcagtccg gtttgctttg cataccggat
      121 tttctttttc ttaccatcct gaagtttttt catcttccct gatttttcct caccatcatt
      181 ggtcattttt cggttgacgc ccttcggctt ttccttcatc tttacatctg gacgtctaaa
      241 cggatggatg tgcacaacac aacatataac tacaagcgat tgatgaggta aggtatgaac
      301 ttttttcacg ccagccagcg ggatgccctg aatcagagcc tggcagaagt ccaggggcag
      361 attaacgttt cgttcgagtt tttcccgccg cgtaccagtg aaatggagca gaccctgtgg
      421 aactccatcg atcgccttag cagcctgaaa ccgaagtttg tatcggtgac ctatggcgcg
      481 aactccggcg agcgcgaccg tacgcacagc attattaaag gcattaaaga tcgcactggt
      541 ctggaagcgg caccgcatct tacttgcatt gatgcgacgc ccgacgagtt gcgcaccatt
      601 gcacgcgact actggaataa cggtattcgt catatcgtgg cgctgcgtgg cgatctgccg
      661 ccgggaagtg gtaagccaga aatgtatgct tctgacctgg tgacgctatt aaaagaagtg
      721 gcagatttcg atatctccgt ggcggcgtat ccggaagttc acccggaagc aaaaagcgcc
      781 caggcggatt tgcttaacct gaaacgcaaa gtcgatgcag gagccaaccg cgcgattact
      841 cagttcttct ttgatgtcga aagctacctg cgttttcgtg accgctgtgt gtcggcgggc
      901 attgatgtgg aaatcattcc gggcatattg ccggtatcta actttaaaca ggcgaagaaa
      961 tttgccgaca tgaccaacgt gcgtattccg gcgtggatgg cgcaaatgtt tgacgggctg
     1021 gatgacgatg ccgaaacccg caagctggtg ggcgcgaata ttgccatgga tatggtgaag
     1081 attttaagcc gtgaaggagt gaaagatttc cacttctata cgcttaaccg cgctgaaatg
     1141 agttacgcga tttgccatac gctgggggtt cgacctggtt tataaatagt gtggctttta
     1201 tgaaaatcac acagtgatca caaattttaa acagagcaca aaatgctgcc tcgaaatgag
     1261 ggcgggaaaa taaggttatc agccttgttt tctccctcat tacttgaagg atatgaagct
     1321 aaaacccttt tttataaagc atttgtccga attcggacat aatcaaaaaa gcttaattac
     1381 gatcaatttg atctacatct ctttaaccaa caatatgtaa gatctcaact atcgcatccg
     1441 tggattaatt caattataac ttctctctaa cgctgtgtat cgtaacggta acactgtaga
     1501 ggggagcaca ttgatgagca cgtcagacga tatccataac acgacagcca ctggcaaatg
     1561 cccgttccat cagggcggtc acgaccagag cgcgggggcg ggcacaacca ctcgcgactg
     1621 gtggccaaat caactccgtg ttgacctgtt aaaccaacat tctaatcgtt ctaacccgct
     1681 gggtgaggac tttgactacc gcaaagaatt cagcaaatta gattactacg gcctgaaaaa
     1741 agatctgaaa gccctgctga cagaatctca gccgtggtgg ccagccgact ggggcagcta
     1801 cgccggtctg tttattcgta tggcctggca cggcgcgggg acttaccgat caatcgatgg
     1861 acgcggtggc gcgggtcgtg gtcagcaacg ttttgcaccg ctgaactcct ggccggataa
     1921 cgtaagcctc gataaagcgc gtcgcttgtt gtggccaatc aaacagaaat atggtcagaa
     1981 aatctcctgg gccgacctgt ttatcctcgc gggtaacgtg gcgctggaaa actccggctt
     2041 ccgtaccttc ggttttggtg ccggtcgtga agacgtctgg gaaccggatc tggatgttaa
     2101 ctggggtgat gaaaaagcct ggctgactca ccgtcatccg gaagcgctgg cgaaagcacc
     2161 gctgggtgca accgagatgg gtctgattta cgttaacccg gaaggcccgg atcacagcgg
     2221 cgaaccgctt tctgcggcag cagctatccg cgcgaccttc ggcaacatgg gtatgaacga
     2281 cgaagaaacc gtggcgctga ttgcgggtgg tcatacgctg ggtaaaaccc acggtgctgg
     2341 tccgacatca aatgtaggtc ctgatccaga agctgcgccg attgaagaac aaggtttagg
     2401 ttgggcgagc acttacggca gcggcgttgg cgcagatgcc attacctctg gtctggaagt
     2461 ggtctggacc cagacgccga cccagtggag caactatttc ttcgagaacc tgttcaagta
     2521 tgagtgggta cagacccgca gtccggctgg cgcaatccag ttcgaagcgg tagacgcacc
     2581 ggaaattatc ccggatccgt tcgatccgtc gaagaaacgt aaaccgacga tgctggtgac
     2641 cgacctgacg ctgcgttttg atcctgagtt cgagaagatc tctcgtcgtt tcctcaacga
     2701 tccgcaggcg ttcaacgaag cctttgcccg tgcctggttc aaactgacgc acagggatat
     2761 ggggccgaaa tctcgttaca tcgggccgga agtaccgaaa gaagatctga tctggcaaga
     2821 tccgctgccg cagccgatct acaacccgac cgagcaggac attatcgatc tgaaattcgc
     2881 gattgcggat tctggtctgt ctgttagtga gctggtatcg gtggcctggg cttctgcttc
     2941 taccttccgt ggtggcgaca aacgcggtgg tgccaacggt gcgcgtctgg cattaatgcc
     3001 gcagcgcgac tgggatgtga acgccgcagc cgttcgtgct ctgcctgttc tggagaaaat
     3061 tcagaaagag tctggtaaag cctcgctggc ggatatcatc gtgctggcag gtgtggttgg
     3121 cgttgagaaa gccgcaagcg ccgctggttt gagcattcat gtaccgtttg cgccgggtcg
     3181 cgttgatgcg cgtcaggatc agactgacat tgagatgttc gaactgctgg agccaattgc
     3241 tgacggtttc cgtaactatc gcgctcgtct ggatgtttcc accaccgagt cactgctgat
     3301 tgataaagcc cagcaactga ccctgaccgc gccggaaatg accgcgctgg tgggcgggat
     3361 gcgtgtactg ggtgccaact tcgatggcag caaaaacggc gtcttcactg accgcgttgg
     3421 cgtattgagc aatgacttct tcgtgaactt gctggatatg cgttacgagt ggaaagcgac
     3481 cgacgaatcg aaagagctgt tcgaaggccg tgaccgcgaa accggcgaag tgaaatacac
     3541 tgccagccgt gcggatctgg tatttggttc taactctgtc ctgcgtgcgg tggctgaagt
     3601 ttacgccagc agcgatgctc acgagaagtt tgttaaagac ttcgtggcgg catgggtgaa
     3661 agtgatgaac ctcgaccgtt tcgacctgct gtaatctgac cttcttcagc gactgccttt
     3721 caggcagtcg ctgaagtttc tttaccggcg tatagtgtcc acaggaaaac tacacactgg
     3781 atctctcatg tctgccgcag gaaagagcaa cccactggca atcagtggcc tggttgtgct
     3841 cacacttatc tggagttata gctggatttt catgaagcaa gtcaccagct acatcggtgc
     3901 cttcgacttt accgccttac gctgcatttt cggcgctctc gttttattca tcgtcctttt
     3961 attacgtggt cgcggaatgc gcccgacacc gtttaaatac accttagcca ttgccctgtt
     4021 acaaacctgc gggatggttg gcctggcgca gtgggcgctg gtcagcggcg gagcggggaa
     4081 ggtggcgatc ctgagctata ccatgccgtt ctgggtggtg attttcgccg cggtgtttct
     4141 cggtgaacgc ctgcgacgtg ggcaatattt cgcgattctg attgccgctt tcggcttatt
     4201 tttggtgttg cagccgtggc aactcgattt ctcttcgatg aaaagtgcca tgctggcaat
     4261 cctctccggt gttagttggg gggcgagcgc gattgttgct aaacgtctgt atgcccgtca
     4321 tccgcgcgtg gatttattgt cgttaacatc ctggcagatg ctctatgcgg cgctggtgat
     4381 gagtgtggtc gctttactgg tgccgcaacg tgaaattgac tggcagccca ctgtgttctg
     4441 ggctctggcc tacagtgcga ttctggcgac ggcgttggcg tggagcttat ggctgtttgt
     4501 gctgaaaaac ttgcctgcca gtattgccag cttaagcaca ctggccgttc ccgtttgcgg
     4561 cgtactcttt tcctggtggc tgctcggtga gaatccgggg gccgttgaag gtagcggtat
     4621 tgtgctgatt gtgctggcac tggcgctggt gagccgtaag aaaaaagaag ccgttagtgt
     4681 aaaaaggatc tgaatttttt cttcatgtgg ggcgatcgct tatttaacaa aataacgata
     4741 atgccccacc atgcgccagc taaacagcac atcttcttcc tgcgcacctg cgccaatgtt
     4801 atgtatcacc agcggcgtac cgtcgcgggc gaagccatct gaaaccaccc caatatgtgc
     4861 cagcccgtta tctaatcgcc aggagacaat atcgcccgct tgatagtcac tggggttctt
     4921 gctgatgggg cgtgttttat cgtggcgggt aaaccaggtt tccagattag gcacccgacg
     4981 gtgatcgatg ttgctgtccg ggcgctttaa ctgccatttt tgcgggtact cggcaaaatt
     5041 cttcgccata tcttcgtgca ccagtttctg caaatcgacc ttctggctgc gcaatgcacg
     5101 gatcaccaca tcggaacata ccccgcgttc ttgcgggaca tcaccgccag gataggtaag
     5161 ctgcacatac gccggatcgt aaaatagcgt gctgccgatt tgctgtctgg caccgtctgc
     5221 gatggcaagg ttggtattgg cctggatttg taccacggtt ggtggaatgg cgggagattt
     5281 taaggagtag ctggtaaatg ccgttagcag gctgaccagc gccagtgaag ctttcatctg
     5341 tttcgtccgt gaaaataaga gtcacagatt cgaccttccg ggctaacgat tgttctgtag
     5401 agaaaatgtc cagaagcgga taaaaaattc ctgaacaacg tgggagtttc agattactgg
     5461 cacagagtgc cggatgcggc gtgaacgtct tatctggcct aaaaagcacg caaattcaat
     5521 atattgcaga gattgcgtag gcctgataag cgtagcgcat caggcaattt tgcgttcaaa
     5581 ctcccggaca agccgggagt ttggagtagg ttattcccac tcttgcagga aacgctgacc
     5641 gtactggtcg gctaccagca gagcggcgta aacctgatct ggcgtcgcgc cgccaggcat
     5701 gttgtggatg gtttcacctt ctgcacatgc cgcttctgcc acaattcgca ttttcgccgg
     5761 gacatcttct ttaatatcca gttgagcgag agttattggc aaacctaccg catggctaag
     5821 cgcagctacg gtttcgattt cctcaaccgg cgagttttcc agtaccagct gcgtcagtgt
     5881 accaaatgcc actttttcac cgtgataata gtgatgggca tcaggaatgg cagtcaggcc
     5941 attatgcacc gcgtgcgctg ccgccagacc accgctttca aagccaacac cgcttaaata
     6001 agtgttcgct tcaatcacgc gttccagtgc cggagtcact acatgctgtt cggcggcgag
     6061 catcgctttt tcgccttctt ccagcaaggt gttgtagcac agttcagcca gcgccagcgc
     6121 cgcctgagtg cacttgccgc ccgccatggt ggtcgcaccg ctacgagagc aggcacgcgc
     6181 ttcaaaccag gtcgccagcg catcgccgat acccgccgct aacagacgcg caggtgcgcc
     6241 cgcgacgatt ttagtatcga caatcaccat attcgggtta tttggcagca gcagatagcg
     6301 gtcaaattca ccttcgtcag tgtagataac ggaaagagcg ctacaaggtg cgtcggttga
     6361 agcaatggtc ggggcaatgg cgaccggaac acccatgaaa tgtgccagtg ctttggctgt
     6421 atcgagggtt tttccgccac cgataccgag aattgcgcca cactgcgcag tctccgcgat
     6481 gccacgcaga cggtcgatct cattttgcga acattcaccg ccaaacggcg caatctctac
     6541 taccagtcca gcatctttaa agcttttctc gacagtggat tgagcaaaac ctaaaacaaa
     6601 tttgtcaccc accactaacc agcgttctgc cagcggcttc aggtattcgc ccagacgatt
     6661 aatcacatca gcgccctgga tgtatttacc cggtgattga ataatgcggt ccataattgc
     6721 tcctttagag atgagtagtg ccaaatgcgg cattccagtc gtgttcgaac ttctctatag
     6781 ctgactctac cgcaggggtg ttgagcattt gttgcgctac atctaagggc agggtgatgg
     6841 attcacatcc tgccagtaaa cagtccagcg cctgacgcgg cgttttaaag ctggccgcca
     6901 gcaccatgct ttctggtgcg tgcatttcta acagcgcttg cagctcctga accgtacgaa
     6961 tgccgtcccc gccctgggca tccacgcggt taacatacgg agcaacgtat tttgcccctg
     7021 ccagtgcggc taataaccct tgtgcggcgc tatatacagc agtgccaagt gtagtaatac
     7081 cctctttttt cagtatttta attgctgcca gaccttcgga agtcaccggg attttcacca
     7141 caatgcccgg aatggcatcg cgcagacgct tcgcttcttc caccatcccc tgcgcgtcgc
     7201 ggctcatggt ctgagcaaac agaatgccct catcaccaat cgctttttgc agacgcggca
     7261 gcacttccca tatggactcc ttgctggcag cgataatgct cgggttagtt gtcaccccgg
     7321 caatcgggaa tatgtgtgcc agacgttcga cttctgcgac gttagcggtg tccagataca
     7381 gttccatgat gtttcctttt acagttccag ttcatgttgc agcaggctgg cgatagcgtc
     7441 ggcagaggcg gcgttaacca gcgcgttacg gaattcttcg tgcatgatgc ggcgagcgag
     7501 gcgcgagaaa atgcgcatat gctgatcgcc cgcagcgtgt ttgttcaggg ttaacataat
     7561 gatgaattgc gcttcatcat cgccccagcg caccggcgct tgcagacgcg ctacgctgat
     7621 ggtggattgc tcaatgtgtt ctgatttgct gtggggaatg gcaaaactaa agcccagacc
     7681 ggtagagaaa acggcctcgc gcgcccacaa gtcggcttcc agtttacgcg gatagcggca
     7741 gcggcccgcc agcagcaggt tatcggtcat gcctttgagc acttcttctt tgctgcgcca
     7801 gtcgctttcc agtgtgatgc actcggcggt gaccagcggt gcgtcttgtt gggtcatgcg
     7861 gaattgcgcc agcaggtgtt ccacttccag cgaagtacgg caggccattg cctggttgag
     7921 caacttgcgg cactcacggc tatcaagttg cgccatccgc gctttcgccg ccggaattga
     7981 tggtgcgctc atgctgagtt catccagccc taagccgacc agcaacggca gcacggaacc
     8041 tttcgctccc agctcaccgc acagaccaat ccatttgccc tggcgatgca ccgcttgcac
     8101 ggcgtaatcg agcgcccgca agaatgccgg attcaggctg ttgtagtgac gagtaacctt
     8161 agcgttatcg cgatccactg ccagcagata ttgcgtcagg tcattactac caatactaaa
     8221 gaaatcaatc tcttcgcagc attgatcgat gatgaacatc accgacggca cttccagcat
     8281 gatgccgagc tggatcttct catcaaacgg aatgtgttcg ttacgcagtt gctgtttggc
     8341 ttccgccagt ttttctttca cccataagat ctcttccatt gaggagatca tcgggatcat
     8401 gattttcagg ctgccgtgag cagaggcacg gaggatcgac cgtagttgtg tggtgaacag
     8461 cgaggcgtat tcttcataaa tacgcaccgc gcgataaccg aggaacgggt tagcttctgc
     8521 cggaatgttc agataatcaa cgggtttgtc accgccaata tccatagtgc gcacaataat
     8581 gctgcgtccg ttggcggact ccagcgcttg acaaaaaatg ttgtacaact cgctttcgcc
     8641 cggtgcgctg gtgcgatcca tatagagcat ttcagtgcgg aacaaaccga cgccttccgc
     8701 accattgccg aatgcggcct gcgcttccac ggagtgagcg atgttagcgg caatttcaat
     8761 gcggataccg tcagcggtac gggcttgttg ggtcagccag acacgctgtt gctcacgcag
     8821 ggcgtcctgt acgcgggctt cttgctgata ataacgagct acggcttccc ctggctcaac
     8881 cacaatcgcc ccggcgttgc cgtcgatata aatcgtttga tgctgccacg gagtaagggc
     8941 atcaatatct acaccaacca gtgtcggaat gttgaacgaa cgggcaagaa ttacggtatg
     9001 tgaggtggtg ccgccacttt tcagtaacag acctttgagg tgatttttat ccagttcgag
     9061 gaactggctg ggggttagtt catcagccat acaaatggcg ggctgcgtca gtttgcccgg
     9121 tgccgggaag cgttgctcac cgtagatttg ctgaagcagc tggaagcaga catcgcgtac
     9181 gtccagggca cgttcttgca ggtagctgct gctggaacgg gaaaactctt cacaaaagtg
     9241 attcgcactg gcgacaatcg cttcggcgca gcttaatccg gcgctgacgc ctgccagtaa
     9301 atgttcgtgc agggaagtat cgccagccag ggatcggtgc gcttccagaa tcgcgctggt
     9361 ggcaccgtcg ctatccagca gacgaaactc aatgtttttc agtaccagcg tcaggccgtt
     9421 ttccagtgcg gattgctcgg cgtcaacgtc tttggctgcg ggaagattac ccagcgcatt
     9481 gagatctaaa gaagagatcg gcgtcagaat gccgcctgcg ctaccgctgc acacggtgcg
     9541 ggcgcggata atttgcggat tcagattggt cagtgaaacc ggcagtggtt ccagttcgtc
     9601 agatttaact tccgccagcg gcgcatcgca gtgggggaat tcatcgcgca gccattggct
     9661 taaacgctgg tgcgcttcct gttcgtcggc cccggaaatc agtaactggc agttatcgcc
     9721 cgccagcgtg tcggtgccaa tcagcgcaag ggcgcttttg gcgttgccct tgcggtcagt
     9781 gcgcaggtta tgccactcaa tttgtgatga aaaagtatta cacagcgttt caacgtggct
     9841 tgccggacgt gcatgtacgc cgttaggtag ctcacaaata aattccacaa tcagggccat
     9901 tgcctctctc ccggtacggt tttctgttta caggataaga gcgcacggca acggcctgcc
     9961 atgtgacaaa tctgccaaaa gctggacaaa tgtaatgtaa ccgtcaattt gcgacgcgtc
    10021 tcacaagacg ccgttttgcg gcatgcttcc ggattatcgc aagttatgag gcggatcgca
    10081 tttttgtact gatattacaa aagtccagta aatggccttt ttatccactg tttgctccgc
    10141 ctgcgattgc ctattgtttc ctccatcaaa aatatggaca tgggctttcg caagcccggg
    10201 agcagcttat gaatgagttg gtgcagatcc tgaaaaatac ccgtcagcat ttaatgacgg
    10261 gcgtttcaca catgattccc ttcgtggtat cgggcggtat tttgctggcg gtttccgtca
    10321 tgttgtatgg caaaggcgca gtgccggatg ccgtagccga tccgaatctg aaaaaactgt
    10381 ttgatatcgg cgttgcaggt ttgacgctga tggtgccttt cctcgccgct tacatcggtt
    10441 actccattgc agagcgttct gcgctggctc cgtgcgctat cggtgcctgg gttggtaaca
    10501 gctttggtgc gggcttcttt ggtgcactga tcgccgggat tatcggcggc atcgtggtgc
    10561 attacctgaa gaaaattccg gtgcataaag ttctgcgctc ggtgatgccc atcttcatca
    10621 ttccgatcgt cggcacactg attaccgcag gcatcatgat gtggggtctg ggcgagcctg
    10681 taggggcgtt gaccaacagc ctgactcagt ggcttcaggg gatgcagcag ggcagcattg
    10741 ttatgctggc ggtgatcatg ggtctgatgc tggcgttcga tatgggcggt ccggttaaca
    10801 aagtggccta tgccttcatg ctgatttgcg ttgctcaggg tgtttatacc gtggtggcca
    10861 ttgctgccgt gggtatttgt atcccgccgc tggggatggg gctggcgacg ctgattggtc
    10921 gtaaaaattt ctccgcagaa gagcgcgaaa ccggtaaagc ggcgctggtg atggggtgcg
    10981 ttggggttac tgaaggggcg attcctttcg ccgcagccga tccgttacgt gtaattcctt
    11041 ccatcatggt tggttcagtt tgtggtgcag taactgcggc gctggtcggt gcgcagtgct
    11101 atgcaggctg gggtggtctg attgtgctgc cggtggttga aggtaagttg ggttatatcg
    11161 cagcagtggc tgtcggggcg gttgtgacgg cagtctgcgt gaacgtgctg aaaagtctgg
    11221 cgcgtaaaaa tggatcttct actgatgaaa aagaagacga cctggatttg gattttgaaa
    11281 ttaattaatt gaggaacgag ccatgacgaa aattattgca gtaaccgcgt gtccatctgg
    11341 tgttgcccat acttatatgg ctgcagaggc gctggaaagt gccgcgaaag cgaaaggctg
    11401 ggaagtgaaa gtagaaaccc agggctcgat tggtctggaa aacgaactga ctgcggaaga
    11461 cgtggcgagc gccgatatgg tgattctgac taaagatatc ggcatcaagt ttgaagagcg
    11521 ttttgctggc aagaccatcg tgcgcgtcaa catcagcgat gcggtaaaac gtgctgatgc
    11581 cattatgagc aaaattgaag ctcacctggc gcaaaccgct taagttcttc tcccgctcgc
    11641 aagggcgggt tcgctttccc acaggagttc ctcatgacga atcgtatctc tcgcctcaaa
    11701 actgcactgt ttgccaatac ccgtgaaatc tcgctggagc gtgcgctgct ttataccgcc
    11761 agccatcggc aaaccgaagg cgaaccggtg atattgcgcc gggcgaaagc aacagcgtat
    11821 atccttgaac atgttgaaat ttcgattcgt gatgaagaac tgattgccgg taaccgcacc
    11881 gtaaaaccgc gcgccgggat tatgtcgccg gaaatggacc cttactggct gctgaaagag
    11941 ctgggtcaat tcccgacgcg tccgcaggac cgttttgcta tcagcgaaga agataaacgt
    12001 atctaccgcg aagagttgtt cccgtactgg gaaaaacgtt cgatgaaaga tttcatcaac
    12061 gggcagatga cggatgaagt aaaagccgcg actagcacgc agattttcag catcaaccag
    12121 acggataaag gccaggggca cattattatt gattacccgc gcctgctgaa tcacgggctg
    12181 ggggagctgg tggcacagat gcagcaacat tgtcagcaac agccggagaa tcacttttat
    12241 caggcagcgc tgttactgct cgaagcctcg cagaaacaca ttttgcgtta cgccgaactg
    12301 gcggaaacga tggcggcaag ctgcactgat gcccagcgtc gcgaagaact gttgactatt
    12361 gcggagatct cccgccataa cgccgaacat aaaccgcaga cgttctggca ggcgtgccag
    12421 ttattctggt acatgaacat cattctgcaa tacgaatcca acgccagttc gctgtcttta
    12481 gggcgcttcg accagtatat gttgccgttc tatcaggcat ctttaaccca gggcgaagat
    12541 ccggcgttcc tgaaagaact gctcgaatct ttatgggtga aatgcaacga cattgtgctg
    12601 ttgcgctcca ccagcagcgc gcgttatttc gcaggtttcc cgactggcta taccgcactg
    12661 ctcggcgggt taaccgagaa cggacgtagc gcggtgaacg tgctttcgtt cctctgcctc
    12721 gatgcctatc aaagcgtaca attaccgcaa ccgaacctag gggtgcgcac taacgccttg
    12781 atcgacacgc cgttcctgat gaaaaccgca gaaaccattc gcctcggcac cggtattccg
    12841 caaatcttta acgatgaagt ggtggtgcca gcgttcctca accgtggcgt ttcgctggaa
    12901 gatgcgcgcg actattccgt agtgggctgt gtggaattat ctattcccgg cagaacctac
    12961 ggcttgcatg acatcgcgat gtttaatctg ctgaaagtga tggaaatctg cctgcatgaa
    13021 aatgaaggca atgctgcgct gacttatgaa ggtttactgg agcagatccg cgccaagatc
    13081 agccactaca tcaccctgat ggttgagggc agcaatattt gtgatatcgg ccatcgcgac
    13141 tgggcacctg taccgctgct ctcgtctttt atcagcgatt gtctggaaaa aggtcgcgat
    13201 attaccgatg gcggcgcgcg ttataacttc tccggcgtac agggaattgg tatcgccaac
    13261 ctgagcgatt ctctccatgc gttgaaaggg atggtttttg atcaacagcg tttaagtttt
    13321 gacgaattgc tgtcggtatt aaaagccaac tttgcaacgc cagaaggcga aaaagtccgc
    13381 gctcgcttaa ttaaccgctt tgagaaatac ggtaacgata tcgacgaggt gggtaacatt
    13441 agcgccgaac tgttgcgcca ctactgcaaa gaagtggaaa aataccagaa cccgcgcggc
    13501 ggttacttca cgccgggatc gtataccgtt tctgctcacg tcccgttggg atcggtggtt
    13561 ggcgcgacgc cagacggtcg ttttgccgga gaacagttgg cagacggcgg cttgtcaccc
    13621 atgctgggcc aggacgcaca agggccaaca gcggtactga agtcagtcag taagctcgat
    13681 aacacgctgc tgtctaacgg tacgttgctg aacgtgaaat tcactccggc gaccctggaa
    13741 ggtgaagcgg gattacgcaa actggccgac ttcttacggg cgtttaccca gcttaagttg
    13801 cagcatattc agtttaacgt ggtgaacgcc gacacgttgc gggaagcgca acagcgccca
    13861 caagattatg ccgggctggt ggtgcgcgtt gccggataca gcgccttctt tgtcgaactg
    13921 tcgaaggaga tccaggatga catcatccgc cggacagcgc atcagctgta acgttgtgga
    13981 aacgcgccgc aatgatgtgg cgcgcatttt caacattcag cgttattcac tgaatgacgg
    14041 cgagggcatt cgtacggtgg tcttttttaa aggctgtccg catctttgcc cgtggtgtgc
    14101 taatccggag tcgatctccg gcaaaatcca gacggtacgc agagaggcga aatgtctgca
    14161 ctgtgcgaaa tgtttgcgtg atgcggatga atgcccctcc ggggcgtttg aacggattgg
    14221 tcgcgatatc agccttgacg cgctggaacg ggaagtgatg aaagatgaca ttttctttcg
    14281 cacgtccggc ggcggcgtca cgctttctgg cggcgaagtg ttaatgcagg cggagtttgc
    14341 tacccgtttt ttacagcggc tgcggttgtg gggcgtgtca tgcgccattg aaactgccgg
    14401 agacgcgcca gccagcaagc tgttaccgct ggcgaaattg tgcgatgaag tgttgttcga
    14461 tttaaaaatt atggacgcga ctcaggcgcg ggatgtggtg aagatgaacc tgccacgcgt
    14521 gctggagaat ctgcgtttgc tggtgagtga gggcgtcaac gtgatcccgc gtttaccgct
    14581 gatccctggt ttcacgctca gccgggagaa tatgcagcag gcgctggatg tactgatccc
    14641 gctgaaaatc aggcagatcc atctgttacc gtttcatcag tacggcgaac cgaaataccg
    14701 cctgctgggg aaaacatggt cgatgaaaga ggtgcctgcg ccgtcgtcag ccgatgtgac
    14761 aacgatgcgc gaaatggcag aacgggccgg atttcaggtt accgtgggag gttaaaatgg
    14821 catacctggt ggcagtaacc gcctgcgtaa gcggcgtggc gcatacatat atggcggcgg
    14881 aacggctgga aaagttgtgc ctgttagaga agtggggagt cagcattgaa actcagggcg
    14941 cgctgggaac ggagaatcgt ttagcggacg aggatatccg tcgggcggat gttgctctgt
    15001 tgattacgga tatcgagctt gccggtgccg agcgatttga acattgccgc tatgtgcaat
    15061 gcagcatcta cgcattcctg cgtgagccgc agcaggtaat gagcgcggtg cgcaaagtgc
    15121 tttctgcgcc gcagcaaacc catcttattc tggagtagtc ggtttttctg tcagttggct
    15181 gtggtactgc cggcgatatt ccgacggcga gcgttcggtg tttttacgaa acagacggca
    15241 gaagtagttg ctgtcgacaa agccgcaggc gtgcgccact tcttttacct tcaggtcata
    15301 gcctttcaat aacgtcttag cgtgctccag tcgcgtgtga ttcaggtatt cgttaaaacc
    15361 aatggccccc gttttttgaa acaggtgcga gagatagttt ggtgaaatat agaatgcctg
    15421 tgcgacagat tcgcgggtaa gcgcggaggc atagcgttcg tcgatataat cgcgaatagc
    15481 ttcgaatagt gcctggctgc gtgaggcggt ctggatttgg ctgccaagca aatcgcggca
    15541 gtggctaagc aggctggcga cgataagcct tgccgtttgc tgctcctgcg gctgcatttg
    15601 catttcattg agcgtttgta gcagaaaaga acctatgcgt gggccgcgtc gggcgacgtg
    15661 ttgcttcgcc agattttgat attgtttgcc atcccattgc acgacgctga agccgagttg
    15721 ctgtttgcca aacagcacgc taaaggtagt agcaggggct tgccattgtg gaaaattcca
    15781 gccaccagcg gcaacgtaca gcacatcgcc gggaactaac gttgctccag cgccggtatc
    15841 aacaaactca ccttccagca caatttccag ccgtggaaaa tcgacctgat acgcaagatc
    15901 gggcaccggg ccgttactgc tggcaaagta aatctggcgc agggacagcg ggccgttgat
    15961 caggcgggag agcagatagc tgacgtcgtg atacatgtta ttcccttagg tatggagacg
    16021 ggaaaacagt aactggcatt attaaaaaag gccacctgaa aagtgtggcc tgaggggagt
    16081 tcgatgtaaa cattgcggca gttgtattgc cggatgtggc gtaaacgcct tatccagtct
    16141 accgccgcga tctgtaggcc ggataagacg cgtcaagcgt cgcatccggc aaatagcgcc
    16201 tggctgataa ttactgatta cctacctggt cgccatacgg cagcgtgtcg taatcgatca
    16261 gcgcgttttt cttgtacggg ttaccaatcc agcgggtatt ttctttgaac tgcggattca
    16321 ccactgaacg ggttggatcg taaccgtcgt aagataagcc cgctaaatct gaccaggtgt
    16381 ggatcagttc cgcaaggctg tatttacgat caacatcctg cgagaaatca cgcggatgag
    16441 tcgcttgcca tttttccgac gtccacagca ggaacggaat ggtgtacata tgacgcgtcg
    16501 ggttgtcctc attacgcccc tgggttttat gaggcggcgt gtcgtaaacc tcttcaccgt
    16561 ggtcagagaa gtaaaccagg aaaccgttcg ggtctgctgc tttaaagtct ttaatcaggc
    16621 tggcaaccac atgatcgtta tacaggttag cgttgtcata atcgttatat gactccagct
    16681 cttccgcgtt taatcccggc ggaacatgat cggtattgcc atcaaacttg ccctgatttt
    16741 ccgggtagcg gtatttgtat ttgatatgcg tacccagcag atgaacgatg atcagtttct
    16801 tcggcgcagg gtccttcagc acttcctgga acggcttcag cacgttagtg tcgtattcac
    16861 gcgcactctg cgtacgttgc tggttcatgt agtactgctt gtcggtctgg cgcgaaaata
    16921 ccgtcagcat ggtattgcgg gcggtcatcg tctgctggtt ggtgatccag aaggttttat
    16981 aacccgcctg tttcatcatg ttcatcagcg acggctgcgt cagatacaga tccgggttct
    17041 tttcattggc aaaggtcagc gcctgttgca ggatttcaat ggtgtacgga cgagacgtaa
    17101 ctacgttatt aaacacggtc agattcggat cggttttatg cagcgcattc agctccggcg
    17161 tggtttcacg cggataaccg tacagactca tgcgtccacg ctgggttgac tcgccaatca
    17221 ccagcactaa ggtgcgcggt tcgttacccg attcatcttt gaaattagcc agcggcggca
    17281 aggcattatt ttcattcagt agctttgtta gcgagtttag ttgctgacga tactgataat
    17341 agccggtcag gaattgccac ggtgcggcag gctccatacg cgaggccagg ttatccaacg
    17401 ttttctcaaa cggcttgttt ttgataaaca tattcatggc gatcggatgc agaatcaagc
    17461 cataaagcag ggcaaaagag acaacataac gccacggctt tggaatatag accgggcgca
    17521 ggcgtgtcca cagcagaact gccaccgccg tataggccag cgcgataagc acaattttca
    17581 ggctgaaata ctggcttaaa tactcgctgg cttcgttggt gttggtttcg aacatcacaa
    17641 acagaacgct ctgcgagaac tcctgaccgt agatgacgta gtagcacagc gccgccagag
    17701 aggccgccca tagcaccacg ccgatcactg cggcaataat tttgatccgc ttcggaaaga
    17761 ggaataccgg aattaaccac agcgagctga ataacagcga gtcgcgaatg ccgttggtgc
    17821 cactataacc actgatgtaa ataatggcct gtagcagagt agagaaaaac caaaagtaga
    17881 gcaatgccca acccagggct ttccagctaa aaagaggttt agcctggact tctgtggaat
    17941 gcatagtaag aacctgtctt gaaaaaaata tcgccgaatt taccgacaat tccttaagga
    18001 tatctgaagg gatatccaga atttgaataa aatgcagaca gaaatatatt gaaaacgagg
    18061 gtgttagaac agaagtattt cagaaaaccc tcgcgcaaaa gcacgagggt ttgcaggaga
    18121 ggaagattag ccggtattac gcatacctgc cgcaatcccg gcaatagtga ccattaacgc
    18181 ctgctcgacg cgaggatccg gttcctggcc ttctttttct gcctggcggg agcggtgcag
    18241 caactcggct tgcaatacgt tcagcgggtc ggtgtaaata ttccgtagct gaatagactc
    18301 tgcaatccac ggcagatcgg ccatcagatg ggaatcgttg gcaatcgcca gcaccacttt
    18361 gatgtcttct tcttgcagat tgcgtaactc tttacctaac ggccacagtg ttttgtctac
    18421 caggcgttgg tcatagtatt ccgccagcca caggtctgct ttggcgaaga ccatctccag
    18481 catgccgaga cgcgtcgaga agaatggcca atcgcggcac atagcctcca gctcgctctg
    18541 tttgccatct tcgaccactt tttgcagcgc cgtacctgca cccagccagg cggggagcat
    18601 cagacggttt tgcgtccagg cgaagatcca cggaatggcg cgtagtgact cgacgccgcc
    18661 ggttgggcga cgtttcgccg gacgtgaacc caacggcagt ttgcccagtt cctgttccgg
    18721 cgtagcggag cggaagtaag gcacaaaatc tttgttttca cgtacgtagc cacggtagag
    18781 atcgcaggag atgactgata gttcatccat aatgcgacgc cagctctctt tcggctcagg
    18841 tggtggcagc aggttggctt ccagtatcgc cccggtgtaa agcgacaggc tgctgacggt
    18901 gatttctggc agaccatatt taaagcggat catctcgccc tgttcagtca cgcgcaggcc
    18961 acctttcagg cttcctggcg gttgtgacag cagcgccgca tgagcaggtg cgccgccgcg
    19021 accaatggaa ccgccgcgac cgtggaacaa cgtcagctca atacccgctt tttcgcaggt
    19081 tttgattaat gcatcctgtg cctgatattg cgcccaggac gctgccatca cgcccgcatc
    19141 ttttgctgag tcggaatagc caatcatcac catctgtttg ccctgaatca ggccgcgata
    19201 ccagtcgata ttcagcaact gagtcatgac atcgttggcg ttgttcaggt catcgagagt
    19261 ttcaaacagc ggagcaaccg gcatcgcaaa cccgataccc gcttctttca gcagcaggtg
    19321 gacagccagc acgtcggacg gcgttttcgc catcgagatc acgtaggcgg caatggagcc
    19381 ttgcggtgct tcggcaatca cctggcaggt atcgagcact tcgcgcgttt cggcgcttgg
    19441 ttgccagttg cgcggcagaa gcggacgttt ggagttcagt tcgcggatca ggaacgcctg
    19501 tttgtcggcc tctgaccagc tttcgtagtc gccgataccg aggtagcggg tcagctcgcc
    19561 cagcgcttcg gtatgacgcg tgctctcctg acggatatca atacggacca gcggtacgcc
    19621 gaaacatttc acgcggcgca gggtgtcgag cagatcgccg ttggcgataa tacccatgcc
    19681 acacgcctga agtgactggt agcaagcgta gagcggttcc cacagttctt cgttttgtgt
    19741 cagcaggcct tctggttttg gcagttcttc gcctttcagg cgcgcttcca gccatgcctg
    19801 tgtcgccatc aggcgagaac gcaggttttt catcagatag cgatacggtt ctgcggcacc
    19861 ttcttcgcca accagcgcca gcagttcagg ggtcgcttca accatcgaca gttcagaaac
    19921 cagaacctga atatctttca ggaacagatc ggtggctttc cagcggctga gcagcaggac
    19981 gtggcgggtg atatcggcag tgacgttcgg gttgccgtcg cggtcaccgc ccatccacga
    20041 ggtaaaacgt accggaacaa attcgacggg cagtttgtag ccgaggttct cttccagttg
    20101 ttcgttcagt tcgcgcaggt aattcggtac gccttgccac aggctgtttt ctactacggc
    20161 aaagccccat ttggcttcat ctaccgggct tggacgcagc ttacggattt catcggtatg
    20221 ccatgactgg gcgatcaact ggcgcaggcg acgcatcagc tggttgtgtt cgtagtcagc
    20281 gatatctttg ttatcgagct gttttaagca ggcgttcact tccaccattt tgtggatcag
    20341 tgtacgacgg gtaatttcgg ttgggtgagc cgtgaggacc agttccagcg acagcgattc
    20401 cactgctttt ttgatggtgt cttcgctcag ttccggctgg tttttcagtt tacgcagggt
    20461 gcgggcgatc acttccgggt tgctggcagc ttcgcctttc ggcgaaatgc tgtggtattg
    20521 ctcggcggtg ttggccaggt tcaggaactg actaaacgca cgcgcaacgg gcagcagctc
    20581 gtcgttcgac aaattttgta aggtggtgag taactcctgg cggttagcat cattgccagc
    20641 gcgtgaagat ttggacaact tacggatagt ttctacgcgt tcaagaatgt gttctcccaa
    20701 cgcatccttg atggtttctc ccagcacttt gccgagcata ctgacattac tacgcaatgc
    20761 ggaatattgt tcgttcatat taccccagac accccatctt atcgtttgat agccctgtat
    20821 ccttcacgtc gcattggcgc gaatatgctc gggctttgct tttcgtcgtc ttttataaag
    20881 ccacgtaaaa gcggtgacgt caaatgctgc gaaatcgctt cagcaaacga ataaatagca
    20941 ggaatttacg tcattaaatt cacgacgctt taaataagcg taacttatgg gaatgttaaa
    21001 aaatcgcctc aagtaacacc aaaggtgtag gtcggataag atgtgcaagc atcgcatccg
    21061 acattattgc ggcactggag tttggcaaca gtgccggatg cggcgcaagc gccttattcg
    21121 gcctacgttt taatgccagc aaaaatggtg aatgacctgg gttatcagtt cgcgggtggg
    21181 cttgataaac cgtgtttcca gatattcatc aggctgatga gcctgattaa ttgagccagg
    21241 ccccagaacc agcgtcgggc ataacgtttg aataaacggc gcttcggtac agtaattcac
    21301 cacttcggtt tttgctccga gcaatttctc aaccacttca accagttgat gattcggtgg
    21361 gcattcatag ccagggatcg gcggatgcag ctcgtcgacc gtcagacgac ccggccagcg
    21421 ttcgctcacc ggagccaatg catcgttgag caaaccatta agttcattga gtgtcatgcc
    21481 aggcagcgga cgaatatcca tatgcaactc acaccaagcg caaatacggt tagaagcgtc
    21541 gccaccgtga atatgcccga ggttgagcgt agggtatggc acggtaaacg cttcgtagtg
    21601 ataacgttct ttcaggttat cgcgcaattg caaaatatgc ccgatggcgt cgtgcattag
    21661 ttctatagcg ttaactccgc gtgctggatc gctggagtgc cccgactggc cctgaatacg
    21721 gatggcgtta gagatatgac ctttatgtgc gcgtaccggt tgtagtgacg tcggttcgcc
    21781 aatgatggcg caatccgggc gcagggcggt agtttcggca aaataacgcg ctccggccat
    21841 actggtttct tcatcagcag tcgccagaat gtagagcggt tttttcagtt tcgtgacgtc
    21901 gacatcgcgt agcgcatcaa ggataaacgc aaaaaagcct ttcatgtcgg cggtgcctaa
    21961 gccgtaaagc ttgccgtcat gctccgtcag tgtaaacgga tcgcgcgtcc agcgaccgtc
    22021 atcaaatggc accgtatcgg tatgccccgc cagcaacaag ccgccagccc cctgtccggt
    22081 acttgccagc atattaaatt tgttgcgagt tcctggtaca ggctgcactt ccacattgaa
    22141 gcctaaatct ttaaaccagt ccgccagcag agtgattaaa tctgcattgc tttgatcgag
    22201 tgcctcttcc gtggcgctta ttgaaggtgt ggcaatcaga gcacggtaaa tctcgataaa
    22261 tggcggtaat ttgtttttca ttgttgacac acctctggtc atgatagtat caatattcat
    22321 gcagtattta tgaataaaaa tacactaacg ttgagcgtaa taaaacccac cagccgtaag
    22381 gtgaatgttt tacgtttaac ctggcaacca gacataagaa ggtgaatagc cccgatgttg
    22441 aatacgctga ttgtgggtgc cagcggctac gctggcgcag agctagtgac ctgtgtaaat
    22501 cgccatccgc atatgaacat aaccgctttg actgtttcag cgcaaagcaa tgatgcggga
    22561 aagttaatct ccgatttgca tccgcagcta aaaggcatcg ttgatctgcc gttgcagccg
    22621 atgtcggata tcagcgagtt tagccctggg gtggacgtgg tgtttctcgc caccgcccat
    22681 gaagttagcc acgatttagc gccgcagttt cttgacgcgg gctgcgtggt gttcgacctt
    22741 tccggcgcgt ttcgtgttaa cgacgccacc ttctatgaaa aatattacgg ctttacccat
    22801 caatacccgg aactgctgga acaggcagcc tacggtctgg cggagtggtg cggtaataaa
    22861 ttaaaagaag cgaatttgat tgcggtgcca ggctgttatc cgacggcagc acagttggcg
    22921 ctgaaaccgt tgattgatgc cgatcttctt gacctcaatc agtggccggt gatcaacgcc
    22981 accagcggcg tgagcggtgc aggacgtaag gcagcgattt caaatagttt ttgtgaagtt
    23041 agcctgcaac cgtatggcgt ctttacccat cgccatcaac cagagatcgc cacacacctc
    23101 ggtgctgacg ttatcttcac cccgcatctg ggcaatttcc cgcgcggcat tctcgaaacc
    23161 attacctgcc gcctgaaacc gggtgtgacc caggcgcaag tggcgcaagc gttacagcag
    23221 gcgtatgccc ataaaccgct ggtgcggctg tatgacaaag gcgttccggc gctgaaaaat
    23281 gtcgttgggc taccattttg cgatatcggg tttgccgttc agggcgaaca tctgattatt
    23341 gtggcgaccg aagacaactt actgaaaggc gcggcggcac aagcggtaca gtgcgccaat
    23401 attcgtttcg gctatgcgga aacgcagtct cttatttaag ggtgcaatga tgaatccatt
    23461 aattatcaaa ctgggtggcg tactgctgga tagtgaagag gcgctggaac gtctgtttag
    23521 cgcactggtg aattatcgtg agtcacatca gcgtccgctg gtgattgtgc acggcggcgg
    23581 ttgcgtggtg gatgagctga tgaaagggct gaatctgccg gtgaaaaaga aaaatggcct
    23641 gcgggtgacg cctgctgatc agattgacat tatcaccgga gcactggcgg gaacggcaaa
    23701 taaaaccctg ttggcatggg cgaagaaaca tcagattgcg gccgtaggtt tgtttctcgg
    23761 tgacggcgac agcgtcaaag tgacccagct tgatgaagag ttaggtcatg ttggactggc
    23821 gcagccaggt tcgcctaagc ttatcaactc cttgctggag aacggttatc tgccggtggt
    23881 cagctccatt ggcgtaacag acgaagggca actgatgaac gtcaatgccg accaggcggc
    23941 aacggcgctg gcggcaacgc tgggcgcgga tctgattttg ctctccgacg tcagtggcat
    24001 tctcgacggc aaagggcaac gcattgccga aatgaccgcc gcgaaagcag aacaactgat
    24061 tgagcagggc attattactg acggcatgat agtgaaagtg aacgcggcgc tggatgctgc
    24121 gcgcacgttg ggccgtccgg tagatatcgc ctcctggcgt catgcggagc agcttccggc
    24181 actgtttaac ggtatgccga tgggtacgcg gattttagct taagttttgt tggccggagg
    24241 cgcagctttc cggcattgaa tttcaaaata aggaaacaga gttatggcac tttggggcgg
    24301 gcgttttacc caggcagcag atcaacggtt caaacaattc aacgactcac tgcgctttga
    24361 ttaccgtctg gcggagcagg atattgttgg ctctgtggcc tggtccaaag ccctggtcac
    24421 ggtcggcgtg ttaaccgcag aagagcaggc gcaactggaa gaggcgctga acgtgctgct
    24481 ggaagatgtt cgcgccaggc cacaacaaat ccttgaaagc gacgccgaag atatccatag
    24541 ctgggtggaa ggcaaactga tcgacaaagt gggccagtta ggcaaaaagc tgcataccgg
    24601 gcgtagtcgt aatgatcagg tagcgactga cctgaaactg tggtgcaaag ataccgttag
    24661 tgagttactg acggctaacc ggcagctgca atcggcgctg gtggaaaccg cacaaaacaa
    24721 tcaggacgcg gtcatgccag gttacactca cctgcaacgc gcccagccgg tgacgttcgc
    24781 gcactggtgc ctggcctatg ttgagatgct ggcgcgtgat gaaagccgtt tgcaggatgc
    24841 gcttaagcgt ctggatgtca gcccgttagg ttgtggcgcg ctggcgggaa cggcttatga
    24901 aatcgaccgt gaacagttag cgggctggct gggatttgct tcagcaaccc gtaacagtct
    24961 cgacagcgtt tctgaccgtg accacgtgct ggaactgctt tctgctgccg ctatcggcat
    25021 ggtgcatctg tcgcgttttg ctgaagatct gattttcttt aacaccggcg aagcggggtt
    25081 tgtggagctt tctgaccgcg tgacttccgg ttcatcatta atgccgcaga agaaaaaccc
    25141 ggatgcgctg gagctgattc gcggtaaatg cggtcgggtg cagggcgcgt taaccggcat
    25201 gatgatgacg ctgaaaggtt tgccgctggc ttacaacaaa gatatgcagg aagacaaaga
    25261 aggtctgttc gacgcgctcg atacctggtt ggactgcctg catatggcgg cgctggtgct
    25321 ggacggcatt caggtgaaac gcccgcgttg ccaggaagcg gcgcaacagg gttacgcgaa
    25381 cgccactgaa ctggcggatt atctggtggc gaaaggtgtg ccgttccgcg aggcacacca
    25441 tattgtgggt gaagctgtgg tggaagccat tcgtcagggc aaaccgctgg aggagctgcc
    25501 gctcaccgag ttgcagaaat ttagtccggt gattggtgaa gatgtctatc cgattctgtc
    25561 gctgcaatcg tgcctcgaca agcgtgcggc aaaaggcggc gtctcaccac agcaggtggc
    25621 gcaggcgatt gcttttgcaa gggcgcggtt ggggtaagaa catttatatg tacaaatttg
    25681 agcctggctt atcgccgggc ttttttatgg caaaaaaaag cggatcctgg agatccgcaa
    25741 aagttcacgt tggctttagt tattcgagtt gagaaactct cgaaacgggc agtgacttca
    25801 agggttaaaa gaggtgccgc tccgtttctg tgagcaatta tcagtcagaa tgcttgatag
    25861 ggataatcgt tcattgctat tctacctatc gccatgaact atcgtggcga tggaggatgg
    25921 ataatgaata ttcgtgatct tgagtacctg gtggcattgg ctgaacaccg ccattttcgg
    25981 cgtgcggcag attcctgcca cgttagccag ccgacgctta gcgggcaaat tcgtaagctg
    26041 gaagatgagc tgggcgtgat gttgctggag cggaccagcc gtaaagtgtt gttcacccag
    26101 gcgggaatgc tgctggtgga tcaggcgcgt accgtgctgc gtgaggtgaa agtccttaaa
    26161 gagatggcaa gccagcaggg cgagacgatg tccggaccgc tgcatattgg tttgattccc
    26221 acggttggac cgtacctgct accgcatatt atcccgatgc tgcaccagac ctttccaaag
    26281 ctggaaatgt atctgcatga agcacagacc caccagttac tggcgcaact ggacagcggc
    26341 aaactcgatt gcgtgatcct cgcgctggtg aaagagagcg aagcattcat tgaagtgccg
    26401 ttgtttgatg agccaatgtt gctggctatc tatgaagacc acccgtgggc gaaccgcgaa
    26461 tgcgtaccga tggccgatct ggcaggggaa aaactgctga tgctggaaga tggtcactgt
    26521 ttgcgcgatc aggcaatggg tttttgcttt gaagccgggg cggatgaaga tacacacttc
    26581 cgcgcgacca gcctggagac gctgcgcaac atggtggcgg caggtagcgg gatcacttta
    26641 ctgccagcgc tggctgtgcc gccggagcgc aaacgcgatg gggttgttta tctgccgtgc
    26701 attaagccgg aaccacgccg cactattggc ctggtttatc gtcctggctc accgctgcgc
    26761 agccgctatg agcagctggc agaggccatc cgcgcaagaa tggatggcca tttcgataaa
    26821 gtgttaaaac aggcggttta aaccgtttaa cgcagctacc cgataggctt ccgccatcgt
    26881 cgggtagtta aaggtggtgt tgacgaagta ctcaatagtg ttgccgccac ctttctgttc
    26941 cataatcgcc tgaccgatat gaataatttc ggcagcgcgc tcgccaaagc agtgaatgcc
    27001 cagaatctct tttgtttccc gatggaacaa aattttcagc gtgcccacgt tcatgccgac
    27061 gatttgtgca cgtgccagat gtttgaactg ggcgcggccc acttcatatg gcactttcat
    27121 cgcggtcagc tgctgttcgg ttttgcccac agagctgatt tccgggatgg tgtaaatgcc
    27181 ggtagggata tcttcaatca gatgcgcggt ggcttcgcct tttaccagcg cctgcgcggc
    27241 aatgcgcccc tggtcatagg ccgctgatgc caggctcgga taaccaatca cgtcgcccac
    27301 cgcgtaaacg tgcggctgtg cggtctgata catgctgttg accttcagct gtccgcgact
    27361 gtcagtttcc agcccaatgt tctgtaacgc cagcgaatcg gtattaccgg tacgaccgtt
    27421 ggcatagagc aggcagtcag ctttcagttt tttacccgac ttcagatgca tgatcacacc
    27481 gtcgtcacag ccttcgatct tctcgtactc ttcgttgtga cgaatcacta cgccactgtt
    27541 ccagaagtga taggagagag aatctgacat ctcttgatcg agaaatgcca gcaggcgatc
    27601 gcgggtgttg atcagatcca cttttacatc cataccgcgg aagatcgacg catattcaca
    27661 gccgatcact ccagcaccat agataagtac atggcgcggt tcgtggtgca tgctgagaat
    27721 tgagtcgctg tcgtaaatgc gtggatgggt gaaatcaaca tctgttggat gatatggacg
    27781 agagccgcag gcaataacaa atttttcagc ggttagtgtt tcaacgctgc cgtccgggca
    27841 atccagcgcc aacgtatgct cgtcaacaaa gcgagcgttc ccctgcaata tttcacagtg
    27901 attacgttcg taaaatccct gacgcatgcg cgtttgttga ttaatcacgt tatcggcatg
    27961 gttaaggata tcggcaaaag aagagcggag cagtcgggaa tggtcgctgt atagtgggtt
    28021 ttgattgaat tctataatgc ggctgacggc gtgacggaga gctttcgacg ggatggtgcc
    28081 ccagtgggtg caaccgccgc caacattttg ataacgctcg ataactgcga cgcgcgcacc
    28141 ttgcttaacc aggcccattg cagcgccttc gccgccgggg ccggaaccta ttactatggc
    28201 atcgtaatcg taggaatgtg gcatggtagg gcttacctgt tcttatacat aaaagcaaca
    28261 gaatggtaac attttatcgc gggtaagcca attgatcccc gtcattaatc tggctatagc
    28321 gtgagcggcc tttgctttgt ctgcctcctt cttaacccat ttattcacta ttaatcgctt
    28381 tttattatgg ctgatagttt tatcttgccg aagcgtgctt ttttgttatt gtctttgtga
    28441 aatcttctta taaaacaaac atataaatat tttatattga tgagttattt gtatttacac
    28501 catcaataaa atccgttaat tagcctgttt ataagtgaat aaaggaatag ccgatgatcg
    28561 aacactatat tcgagggttt gaggaagaac tgcgtgagat ccgccatcag atccacgaaa
    28621 acccggagct gggattacaa gaatttaaaa ccagtgcttt ggttgcggaa aagctgcgtc
    28681 agtggggcta tgaagtagag caaggactgg cgacgacggg gatagtggcc acgttaaaag
    28741 tgggcgacgg agagaaaagt attggcctgc gggcggatat ggatgcgctg ccgatttatg
    28801 aaaatagtgg caagccctgg gccagtaaac atcctggttt aatgcacgcc tgcgggcatg
    28861 acgggcatac caccatactg ctgggggccg cgcgttattt tgccgaaacc cgccgtttta
    28921 acggcacgtt acgtcttatc ttccagcctg cggaagagat gattaatggc ggtgagatca
    28981 tggttaaaga ggggcttttt gatcatttcc cctgcgatgt cattttcggc atgcataaca
    29041 tgcccggtct gccggtgggt aagttttatt tccagccagg ggcactgatg gcgtcaatgg
    29101 atcagttcca tattacagtt cgtggttgtg gcggacacgg tgcgatcccg cacaaagcca
    29161 tcgatccggt gctagttgcc gcacatatca ccaccgcatt acaaagcatt gtgtcacgca
    29221 atgtcgatcc gctggaagcg gcggtaatta ccgtcggcag cattgttgcg ggtgaggcgg
    29281 ctaacgtcat tcctgacagt gctgagatga aaatcagcgt ccgctctctt agccgcgata
    29341 cccgacaact tcttctgacc cgtattcctg ctctggcaca agcccaggct gccagctttg
    29401 gcgctacggc tgaggttacg catgttaacg gtacaccagt attagtaaat gacgaggaga
    29461 tggcgcgctt cgcctggcag gtggcgtgta aaacgtttgg tgaagatcgg gccgagtttg
    29521 gcatcaagcc cctgatgggc agtgaagatt tctctttcat gctggaagcc caacccaaag
    29581 gcggtttcct gttattcggt aatggcgatg tcggggaagg ttcctgcatg gtgcataacc
    29641 cgggttacga cttcaacgat gcaagtctgg tcccggcaag cagctactgg ggcgcactgg
    29701 tagaagcctg gctgcaataa ataaaacaac acacaacaac actttttggc aaggaacatg
    29761 gaatgacggc gatcgtaaat gaaaccgtga gtgtgaagaa aaagcccagc ggcaggcggg
    29821 tgatttttgc ctcggcgttt ggcaatgcgc tcgaattttt tgattttggc gtttacaact
    29881 tttttgtggt ctatatcagc acgctatttt ttccgcccag tgcagataaa aacgtcgcgc
    29941 tactgctggc gtttgccact ttcggcgtga gcttttttat gcgcccgctc ggcggcatta
    30001 ttgtgggggc ctgggccgat cgtttcggac gcaaacccgc gatggtgttc accattgcgc
    30061 tgatgagtct tggtacactg atgatcggca tcgcaccgac gtatgaaacg gcgggctatt
    30121 ggggaaccgc gacgctggtg ctagcgcgtc taattcaggg cgttgcggca ggaggcgaag
    30181 tcggtgcctc aatgtctttg ttggttgaat cagctccggc aaatcgtcgc ggtttttaca
    30241 gcagttggtc actggcgact caaggcctgg caacaacttt tggaggtgtt gtcgctttag
    30301 gtttaagtgc atggctgccc tttgcgaccg gttcagaaac cgtcatggct gaatggggct
    30361 ggcgtgtgcc gttttttatt ggcgtgttat tagcgcctgt cggctgttgg ttgcgtttga
    30421 gtctggaaaa tgatgttcct gagccagcac ataataagaa ggccgcagcc agcgaaagcg
    30481 ccttctcctt acttatgcaa cataaagcga ctatcgctaa cggtatcttg ctcgctattg
    30541 gtagtacagt cgcaacctat atctccctct tttattacgg tacatgggcg gcgaagtatt
    30601 taggaatgaa ccaaaactat tctcatgcag cgatgttact ggcgggcgtt attacgtttg
    30661 tgggtgcgct gctggtgggg atgttgtgcg attcggtcgg gcgtaaaaag ctgattttaa
    30721 tctcccgtgt gatggtgctg atctgtagct ggccgtcatt ctggctgttg gtgaattacc
    30781 caagcccagg catgttgctg acggtggttt tcgtgatggt cagctttacc acgcttggcg
    30841 gtgtgccagt gatgttgttg atctctgaac tgttgccgaa acggattcgg gcattgggtt
    30901 ttgcgctggt ttatagcatt ggtgtggcaa tctttggcgg ttttgctcaa tatttcgcta
    30961 cgcaatccat cgtgttgctg gatagtttga cggctccggc gtggtatctg ggtggaggca
    31021 cgttgctgtc gatgttggcg ttgttgtacg taaaagaacc ggccaaagaa ttgcagtaaa
    31081 tatgttttat tgcgttaccg ttcattcaca atactggagc aatccagtat gttcattctc
    31141 tggtatagtg ccagcagtac ttttggcaag gattcagaca tcgtgatggg cgtaagagcg
    31201 caacaaaaag aaaaaacccg ccgttcgctg gtggaagccg catttagcca attaagtgct
    31261 gaacgcagct tcgccagcct gagtttgcgt gaagtggcgc gtgaagcggg cattgctccc
    31321 acctcttttt atcggcattt ccgagacgta gacgaactgg gtctgaccat ggttgatgag
    31381 agcggtttaa tgctacgcca actcatgcgc caggcgcgtc agcgtatcgc caaaggcggg
    31441 agtgtgatcc gcacctcggt ctccacattt atggagttca tcggtaataa tcctaacgcc
    31501 ttccggttat tattgcggga acgctccggc acctccgctg cgtttcgtgc cgccgttgcg
    31561 cgtgaaattc agcacttcat tgcggaactt gcggactatc tggaactcga aaaccatatg
    31621 ccgcgtgcgt ttactgaagc gcaagccgaa gcaatggtga caattgtctt cagtgcgggt
    31681 gccgaggcgt tggacgtcgg cgttgaacaa cgtcggcaat tagaagagcg actggtactg
    31741 caactgcgaa tgatttcgaa aggggcttat tactggtatc gccgtgaaca agagaaaacc
    31801 gcaattattc cgggaaatgt gaaggacgag taatgaaaca agcaaatcaa gatagaggta
    31861 cgctgctgct ggcgttggtt gctggcttat cgattaatgg tactttcgca gcgctgttta
    31921 gctccattgt gccattttct gtattcccga ttatttccct ggtgctgacg gtttactgcc
    31981 tgcatcaacg ttatcttaat cgcaccatgc cggtaggctt gccgggtctg gcagctgcct
    32041 gttttattct cggcgtactg ctgtacagca cggtagttcg tgcggaatat ccggatatcg
    32101 gctctaactt cttcccggca gtactctccg tcattatggt gttctggatt ggcgcgaaga
    32161 tgcgtaaccg taagcaggaa gttgctgagt aatcggtatc atgccggatg ctggcgcatc
    32221 cggcatgggt tttacttcgc ggtcagtaat acgctgcact ccatatggtg cgtgtagggg
    32281 aactgatcaa acagagccag acgttcgacc ttgtgcgtct ggcttaatgt ttccagattt
    32341 ttgcataacg tttccggatt gcaggagata tacagaatac gtggatacgc ctgcaccatt
    32401 ttctcggttt cactgtccag accgctgcga ggtgggtcga caaaaatggt ttcgcactga
    32461 taactcttta agtcgatccc ttgcaggcgg ttaaactcgc gcacgccatt catcgcctga
    32521 gtaaattctt ctgccgccat acgaataatt tgtacgttat caatatggtt agcggcaatg
    32581 ttgtattgcg cggcagcgac tgatggcttg gcgatttcgg tggctaatac ccgatcaaaa
    32641 ttgcgcgcca gcgctaatga gaagttaccg ttgccgcagt acagctccag taaatcgcct
    32701 tttgagcctt tggttacgtc cagcgcccat tccagcatct gaatattcat cgccgcgttc
    32761 ggctgggtaa agctgttttc tacctgacga tagatcatct ctttccctgc gaccggcaga
    32821 cgctcgtcga tgtaatcttg atccagcgcg attttggttt tcgttgcccg accaatcaga
    32881 tgcacattca gattctgcgc gcgcagtgca tcgcgcaggg cttccgcttc ctgacgccac
    32941 tcatcatcca gctttttatg gtacagcagg gaaaccaccg cctgattact cagcgtggtg
    33001 aggtaatcaa tctggaacaa cttgtggcgc agaacgggat tattacgcac acccgcaatc
    33061 atcgccgtca tcaactggtt gatgagttca ctggcggcgg ggaagctatc cacccggata
    33121 cggcttttgg tttgttgatc gaaaatgatg tggtacaggt catcgccatc gtgccagatg
    33181 cggaactccg cacgcatccg gtaatgactg accggcgagc gaaacacttc cggaaccagg
    33241 tcagaaaacg gtgccatcat actttgcaaa cgtaccactt tttcggctaa ctgcgcttca
    33301 tactgttctg ttggaaggtg ttcgggggtc atgatgtatc ctgaaaaatt aaagtacgcg
    33361 gcgattgtag ggattgctca tcagatgtcc agatcttgat gaattcctat ttgtgagcta
    33421 cgtctggaca gtaacttgtt acaacctgta gcatccactt gccggtcctg tgagttaata
    33481 gggaatccag tgcgaatctg gagctgacgc gcagcggtaa ggaaaggtgc gatgattgcg
    33541 ttatgcggac actgccattc ggtgggaagt catcatctct tagtatctta gatacccctc
    33601 caagcccgaa gacctgccgg ccaacgtcgc atctggttct catcatcgcg taatattgat
    33661 gaaacctgcg gcatccttct tctattgtgg atgctttaca atgattaaaa aagcttcgct
    33721 gctgacggcg tgttctgtca cagccttttc cgcttgggca caggatacca gcccggatac
    33781 tctcgtcgtt actgctaacc gttttgaaca gccgcgcagc actgtgcttg caccaaccac
    33841 cgttgtgacg cgtcaggata tcgaccgctg gcagtcgacc tcggttaatg atgtgctgcg
    33901 ccgtcttccg ggcgtcgata tcacccaaaa cggcggttca ggtcagctct catctatttt
    33961 tattcgcggt acaaatgcca gtcatgtgtt ggtgttaatt gatggcgtac gcctgaatct
    34021 ggcggggggg agtggttctg ccgaccttag ccagttccct attgcgcttg tccagcgtgt
    34081 tgaatatatc cgtgggccac gctccgccgt ttatggttcc gatgcaatag gtggggtggt
    34141 gaatatcatc acgacgcgcg atgaacccgg aacggaaatt tcagcagggt ggggaagcaa
    34201 tagttatcaa aactatgatg tctctacaca gcaacaactg ggggataaga cacgagtaac
    34261 gttgttgggc gattatgccc atactcatgg ttatgatgtt gttgcctatg gtaataccgg
    34321 aacgcaagcg cagccagata acgatggttt tttaagtaaa acgctttatg gcgcgctgga
    34381 gcataacttt actgatgcct ggagcggctt tgtgcgcggc tatggctatg ataaccgtac
    34441 caattatgac gcgtattatt ctccgggttc accattggtc gatacccgta aactctatag
    34501 tcaaagttgg gacgccgggc tgcgatataa cggcgaactg attaaatcac aactcattac
    34561 cagctatagc catagcaaag attacaacta cgatccccat tatggtcgtt atgattcgtc
    34621 ggcgacgctc gatgagatga agcaatacac cgtccagtgg gcaaacaaca tcatcattgg
    34681 ccacggtaat gttggtgcgg gtgttgactg gcagaagcag agcacggcac cgggcacagc
    34741 ttatgttaag gatggatatg atcaacgtaa taccggcatc tatctgaccg ggctgcaaca
    34801 agtcggcgat tttacctttg aaggcgcagc acgcagcgac gataactcac agtttggtcg
    34861 tcatggaacc tggcaaacca gcgccggttg ggaattcatc gaaggttatc gcttcattgc
    34921 ttcctacggg acatcttata aggcaccaaa tctggggcaa ctgtatggct tctacggaaa
    34981 tccgaatctg gacccggaga aaagcaaaca gtgggaaggc gcgtttgaag gcttaaccgc
    35041 tggggtgaac tggcgtattt ccggatatcg taacgatgtc agtgacttga tcgattatga
    35101 tgatcacacc ctgaaatatt acaacgaagg gaaagcgcgg attaagggcg tcgaggcgac
    35161 cgccaatttt gataccggac cactgacgca tactgtgagt tatgattatg tcgatgcgcg
    35221 caatgcaatt accgacacgc cgttgttacg ccgtgctaaa cagcaggtga aataccagct
    35281 cgactggcag ttgtatgact tcgactgggg tattacttat cagtatttag gcactcgcta
    35341 tgataaggat tactcatctt atccttatca aaccgttaaa atgggcggtg tgagcttgtg
    35401 ggatcttgcg gttgcgtatc cggtcacctc tcacctgaca gttcgtggta aaatagccaa
    35461 cctgttcgac aaagattatg agacagtcta tggctaccaa actgcaggac gggaatacac
    35521 cttgtctggc agctacacct tctgaaccac gtcccaccgt gctggtgttt gactccggcg
    35581 tcggtgggtt gtcggtctat gacgagatcc ggcatctctt accggatctc cattacattt
    35641 atgctttcga taacgtcgct ttcccgtatg gcgaaaaaag cgaagcgttt attgttgagc
    35701 gagtggtggc aattgtcacc gcggtgcaag aacgttatcc ccttgcgctg gctgtggtcg
    35761 cttgcaacac tgccagtacc gtttcacttc ctgcattacg cgaaaagttc gacttcccgg
    35821 ttgttggtgt cgtgccggcg attaaacctg ctgcacgtct gacggcaaat ggcattgtcg
    35881 gattactggc aacccgcgga acagttaaac gttcttatac tcatgagctg atcgcgcgtt
    35941 tcgctaatga atgccagata gaaatgctgg gctcggcaga gatggttgag ttggctgaag
    36001 cgaagctgca tggcgaagat gtttctctgg atgcactaaa acgtatccta cgcccgtggt
    36061 taagaatgaa agagccgcca gataccgttg tattgggttg cacccatttc cctctactac
    36121 aagaagaact gttacaagtg ctgccagagg gaacccggct ggtggattct ggcgcagcga
    36181 ttgctcgccg aacggcctgg ttgttagaac atgaagcccc ggatgcaaaa tctgccgatg
    36241 cgaatattgc cttttgtatg gcaatgacgc cagaagctga acaattattg cccgttttac
    36301 agcgttacgg cttcgaaacg ctcgaaaaac tggcagtttt aggctgattt ggttgaatgt
    36361 tgcgcggtcg gaaaattatt ttaaatttcc tcttgtcagg ccggaataac tccctataat
    36421 gcgccaccac tgacacggaa caacggcaaa cacgccgccg ggtcagcggg gttctcctga
    36481 gaattccg
//
LOCUS       DBFIFI010000026        35936 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.026, whole genome shotgun sequence.
ACCESSION   DBFIFI010000026 DBFIFI010000000
VERSION     DBFIFI010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35936
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.026"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            67..1722
                     /gene="aslA"
                     /locus_tag="N7B39_001261"
     CDS             67..1722
                     /gene="aslA"
                     /locus_tag="N7B39_001261"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase AslA"
                     /protein_id="HGU2421276.1"
                     /translation="MEFSFSPKLLVVAVAAALPLMASAADTPSTATARKGFAGYDHPN
                     QYLVKPATTIADNMMPVMQHPAQDKEPQQKLAELEKKTGKKPNVVVFLLDDVGWMDVG
                     FNGGGVAVGNPTPDIDAVASQGLILTSAYSQPSSSPTRATILTGQYSIHHGILMPPMY
                     GQPGGLQGLTTLPQLLHDQGYVTQAIGKWHMGENKESQPQNVGFDDFRGFNSVSDMYT
                     EWRDVHVNPEVALSPDRSEYIKQLPFSKDDVHAVRGGEQQAIADITPKYMEDLDQRWM
                     EYGVKFLDKMAKSDKPFFLYYGTRGCHFDNYPNAKYAGSSPARTSYGDCMVEMNDVFA
                     NLYKALEKNGQLDNTLIVFTSDNGPEAEVPPHGRTPFRGAKGSTWEGGVRVPTFVYWK
                     GMIQPRKSDGIVDLADLFPTALDLAGHPGAKVANLVPKTTFIDGVDQTSFFLGTNGQS
                     NRKAEHYFLNGKLAAVRMDEFKYHVLIQQPYAYTQSGYQGGFTGTVMQTAGSSVFNLY
                     TDPQESDSIGVRHIPMGVPLQTEMHAYMEILKKYPPRAQIKSD"
     gene            complement(1890..3125)
                     /gene="aslB"
                     /locus_tag="N7B39_001262"
     CDS             complement(1890..3125)
                     /gene="aslB"
                     /locus_tag="N7B39_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HGU2421277.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETTAREGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVSK"
     gene            complement(3272..3348)
                     /locus_tag="N7B39_001263"
     tRNA            complement(3272..3348)
                     /locus_tag="N7B39_001263"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3312..3314),aa:Pro,seq:tgg)
     gene            complement(3391..3477)
                     /locus_tag="N7B39_001264"
     tRNA            complement(3391..3477)
                     /locus_tag="N7B39_001264"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3441..3443),aa:Leu,seq:cag)
     gene            complement(3498..3573)
                     /locus_tag="N7B39_001265"
     tRNA            complement(3498..3573)
                     /locus_tag="N7B39_001265"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3538..3540),aa:His,seq:gtg)
     gene            complement(3632..3708)
                     /locus_tag="N7B39_001266"
     tRNA            complement(3632..3708)
                     /locus_tag="N7B39_001266"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3672..3674),aa:Arg,seq:ccg)
     gene            complement(3811..5196)
                     /gene="yifK"
                     /locus_tag="N7B39_001267"
     CDS             complement(3811..5196)
                     /gene="yifK"
                     /locus_tag="N7B39_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HGU2421278.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(5387..6127)
                     /gene="wecG"
                     /locus_tag="N7B39_001268"
     CDS             complement(5387..6127)
                     /gene="wecG"
                     /locus_tag="N7B39_001268"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HGU2421279.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEENAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTTVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(6130..7482)
                     /gene="wzyE"
                     /locus_tag="N7B39_001269"
     CDS             complement(6130..7482)
                     /gene="wzyE"
                     /locus_tag="N7B39_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HGU2421280.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(7479..8558)
                     /gene="rffT"
                     /locus_tag="N7B39_001270"
                     /gene_synonym="wecF"
     CDS             complement(7479..8558)
                     /gene="rffT"
                     /locus_tag="N7B39_001270"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HGU2421281.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFYWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDASLNTMANDRQREGKMTILVGNSGDRSNEHIAALRAVHQQFGDTVKVVVP
                     MGYPPHNEAYIEEVRQAGLELFSEENLQVLSEKLEFDAYLTLLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(8555..9805)
                     /gene="wzxE"
                     /locus_tag="N7B39_001271"
     CDS             complement(8555..9805)
                     /gene="wzxE"
                     /locus_tag="N7B39_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HGU2421282.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISLGLFGNHDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAVMLIKRGAIPLSYLKPSWDNGLAGQLS
                     KFTLMTLITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPTHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(9807..10937)
                     /gene="rffA"
                     /locus_tag="N7B39_001272"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(9807..10937)
                     /gene="rffA"
                     /locus_tag="N7B39_001272"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HGU2421283.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCLQNAHMFYIKLRDIGDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(10942..11616)
                     /gene="rffC"
                     /locus_tag="N7B39_001273"
                     /gene_synonym="wecD"
     CDS             complement(10942..11616)
                     /gene="rffC"
                     /locus_tag="N7B39_001273"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HGU2421284.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPFLTPDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNASDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASSRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(11594..12475)
                     /gene="rfbA"
                     /locus_tag="N7B39_001274"
     CDS             complement(11594..12475)
                     /gene="rfbA"
                     /locus_tag="N7B39_001274"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HGU2421285.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEVATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(12494..13561)
                     /gene="rffG"
                     /locus_tag="N7B39_001275"
     CDS             complement(12494..13561)
                     /gene="rffG"
                     /locus_tag="N7B39_001275"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HGU2421286.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNALTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPQGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWRKQVQDGSYQGERLGLKG"
     gene            complement(13561..14823)
                     /gene="wecC"
                     /locus_tag="N7B39_001276"
     CDS             complement(13561..14823)
                     /gene="wecC"
                     /locus_tag="N7B39_001276"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HGU2421287.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(14820..15950)
                     /gene="wecB"
                     /locus_tag="N7B39_001277"
     CDS             complement(14820..15950)
                     /gene="wecB"
                     /locus_tag="N7B39_001277"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HGU2421288.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(16006..17052)
                     /gene="wzzE"
                     /locus_tag="N7B39_001278"
     CDS             complement(16006..17052)
                     /gene="wzzE"
                     /locus_tag="N7B39_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HGU2421289.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(17064..18167)
                     /gene="wecA"
                     /locus_tag="N7B39_001279"
     CDS             complement(17064..18167)
                     /gene="wecA"
                     /locus_tag="N7B39_001279"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HGU2421290.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGNLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFLLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(18407..19666)
                     /gene="rho"
                     /locus_tag="N7B39_001280"
     CDS             complement(18407..19666)
                     /gene="rho"
                     /locus_tag="N7B39_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HGU2421291.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(19751..19852)
                     /gene="rhoL"
                     /locus_tag="N7B39_001281"
     CDS             complement(19751..19852)
                     /gene="rhoL"
                     /locus_tag="N7B39_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HGU2421292.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(19993..20322)
                     /gene="trxA"
                     /locus_tag="N7B39_001282"
     CDS             complement(19993..20322)
                     /gene="trxA"
                     /locus_tag="N7B39_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HGU2421293.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            20453..21718
                     /gene="rhlB"
                     /locus_tag="N7B39_001283"
     CDS             20453..21718
                     /gene="rhlB"
                     /locus_tag="N7B39_001283"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HGU2421294.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHCLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            21854..23338
                     /gene="gppA"
                     /locus_tag="N7B39_001284"
     CDS             21854..23338
                     /gene="gppA"
                     /locus_tag="N7B39_001284"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HGU2421295.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(23385..25406)
                     /gene="rep"
                     /locus_tag="N7B39_001285"
     CDS             complement(23385..25406)
                     /gene="rep"
                     /locus_tag="N7B39_001285"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HGU2421296.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            25623..26072
                     /locus_tag="N7B39_001286"
     CDS             25623..26072
                     /locus_tag="N7B39_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HGU2421297.1"
                     /translation="MAINFSPKVGEILECNFGNYPVSQNGPFSTTYYDGRIPPEMIKN
                     RLVVVLNGKINGNAFIVVPLSTTRDHDKLKRGMHVEIASNVINDLQFFDQQIRWAKTD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            26271..26552
                     /gene="ppiC"
                     /locus_tag="N7B39_001287"
     CDS             26271..26552
                     /gene="ppiC"
                     /locus_tag="N7B39_001287"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HGU2421298.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(26598..28073)
                     /gene="ilvC"
                     /locus_tag="N7B39_001288"
     CDS             complement(26598..28073)
                     /gene="ilvC"
                     /locus_tag="N7B39_001288"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HGU2421299.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVHTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDLNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            28223..29116
                     /gene="ilvY"
                     /locus_tag="N7B39_001289"
     CDS             28223..29116
                     /gene="ilvY"
                     /locus_tag="N7B39_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HGU2421300.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(29168..30712)
                     /gene="ilvA"
                     /locus_tag="N7B39_001290"
     CDS             complement(29168..30712)
                     /gene="ilvA"
                     /locus_tag="N7B39_001290"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HGU2421301.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(30715..32565)
                     /gene="ilvD"
                     /locus_tag="N7B39_001291"
     CDS             complement(30715..32565)
                     /gene="ilvD"
                     /locus_tag="N7B39_001291"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HGU2421302.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHSDRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDTLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQISFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(32630..33559)
                     /gene="ilvE"
                     /locus_tag="N7B39_001292"
     CDS             complement(32630..33559)
                     /gene="ilvE"
                     /locus_tag="N7B39_001292"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HGU2421303.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(33579..33842)
                     /gene="ilvM"
                     /locus_tag="N7B39_001293"
     CDS             complement(33579..33842)
                     /gene="ilvM"
                     /locus_tag="N7B39_001293"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HGU2421304.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(33839..35485)
                     /gene="ilvG"
                     /locus_tag="N7B39_001294"
     CDS             complement(33839..35485)
                     /gene="ilvG"
                     /locus_tag="N7B39_001294"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HGU2421305.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLKLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(35488..35538)
                     /gene="ilvX"
                     /locus_tag="N7B39_001295"
     CDS             complement(35488..35538)
                     /gene="ilvX"
                     /locus_tag="N7B39_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HGU2421306.1"
                     /translation="MNNSTKFCFPRFRTGN"
     gene            complement(35625..35723)
                     /gene="ilvL"
                     /locus_tag="N7B39_001296"
     CDS             complement(35625..35723)
                     /gene="ilvL"
                     /locus_tag="N7B39_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HGU2421307.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
BASE COUNT         8711 a         9885 c         9082 g         8258 t
ORIGIN      
        1 agggatctca attatcaatg cttaattacg tcatcatttt gttgacatga aaccggagca
       61 ttttgcatgg aattttcgtt ttcacccaaa cttcttgttg ttgctgtcgc cgccgctctt
      121 cctctcatgg ccagcgcagc agataccccg tcaactgcca ccgcgcgcaa aggctttgcc
      181 ggatacgatc acccaaacca gtatctggtt aaaccggcga ccactattgc cgacaacatg
      241 atgccagtaa tgcagcatcc ggcgcaggat aaagaacccc agcagaagct ggcagaactt
      301 gagaaaaaaa ccggtaagaa accgaatgtg gttgttttct tgctggacga tgtgggctgg
      361 atggacgtcg gttttaacgg tggcggcgtg gcggtgggta accctacacc agatatcgac
      421 gccgttgcca gccaggggct gattttaact tcggcgtatt ctcaaccaag ctcttcccca
      481 acccgcgcca ccattctcac cggacaatac tccatccacc acggcattct gatgccgcca
      541 atgtacgggc aaccgggcgg gctgcaaggg ttaaccacgc tgccgcagtt gctgcacgat
      601 cagggctacg tcactcaggc catcgggaaa tggcatatgg gggaaaacaa agagtcgcag
      661 ccgcagaacg ttggctttga tgatttccgt ggctttaact cggtgtctga tatgtacacc
      721 gaatggcgcg acgttcacgt caatccggaa gtggccctga gtccggaccg ttctgaatac
      781 atcaagcaat taccatttag caaagatgat gttcatgcgg tgcgcggcgg cgaacaacag
      841 gccattgccg acattacgcc gaaatatatg gaagatctgg atcaacgctg gatggaatat
      901 ggcgttaagt tcctcgacaa gatggcgaag agtgataagc ctttcttcct ctattacggc
      961 actcgcggct gtcacttcga taactacccg aacgccaaat atgcgggtag ctctccggca
     1021 cgcacctcgt atggcgactg catggtggaa atgaacgatg tgttcgctaa tctgtataaa
     1081 gcactggaga aaaacggtca gcttgataac acgctgattg tctttacctc cgataacgga
     1141 ccggaagccg aagtaccgcc gcacggacgc acaccgttcc gtggtgcgaa aggatctacc
     1201 tgggaaggcg gcgttcgcgt accgactttc gtttactgga aaggcatgat ccaaccgcgt
     1261 aaatctgacg gtattgttga tctggcagat ctcttcccaa cagcactgga tctggcgggg
     1321 catcctggtg cgaaagtggc gaatttagtg ccgaaaacca cctttatcga tggtgtggac
     1381 cagacatctt tcttcctggg aacaaatggt cagtctaacc gtaaggccga gcactacttc
     1441 ctcaacggta aactcgctgc tgtgcgtatg gatgaattca agtatcacgt cctgattcag
     1501 caaccttacg cttataccca gagcggatat cagggtggat tcaccggcac agtaatgcaa
     1561 acagccggat cgtcggtgtt taacctctac accgatccgc aggagagcga ctctatcggc
     1621 gtgcgccata ttccgatggg tgtaccgcta cagaccgaaa tgcacgcgta tatggagatc
     1681 ctgaaaaagt atccaccacg cgcgcagatt aaatctgatt aagccggcgc tatcgctgag
     1741 atctgccttt gccggatgcg atgctgacgc atcttatcca gcctacagaa cgctgcaatt
     1801 tattgaattt gcacgatcat gtaggccgga taaggcgttt acgccgcatc cggcaatcaa
     1861 ccgcaggcga ccacaaaatg gccgcttttt tacttactca ccaccagcaa atgcgcctgc
     1921 ataatgtcgc tggccgggcg accgtgcgcc agcaaatcag ccattgcttt aagatatggc
     1981 ggtagatggc ggaaataacg ctgatacccg gcacacaaat aattcagtcc cggtttgccg
     2041 ctggcatcga gcatgaagcg gtgtttcggg cagcctcccc aacatgcttt taacacgtta
     2101 caactgcgac actgcgccgg taattgctta aatttatctt caccaaacac ctgctgttgc
     2161 ggggaatcga tcatttctgc aattgtttgc tggtgcatat tccccagtcg atattgcgga
     2221 taaacatagt gatcgcaggc gtaaacgtcg ccattgtgct caacaatcac cgagcgccca
     2281 caggttggct gatgatggca aaccgcaccc ggcgcaccga caaaattggc aaacgcccat
     2341 tcgatattca tcacgaaaat cttgccgacg tcgcgtttaa tccagtggtc gaatatcgcc
     2401 accagaaatt caccaaattc atcggggcgc acagaccatt ccgtcagttc cccctgaata
     2461 tcaccaggcg catgtagttt cagtccttcg cgggcagtcg tttcatcagc caggcgctcg
     2521 accaccggaa taaactggat gaattcgact cccgcatcgc acaaaaaatc ataaacctgt
     2581 aacggttgct gcgcgctggt gcggttgacg cagaccagca cgttatagtc gacatgatgt
     2641 ttttgcagga gcgtcagggc acgcatcacc agtttatgcg tgggtctgcc gccttttgtc
     2701 acgcgatatt gattgtggat ctcagccgga ccatccagcg ataacccaac aagaaaatga
     2761 ttttctgcca gaaatgcaca ccattcatca tcgagcagta cgccgttagt ctggaagctg
     2821 ttacttatct tcctgccagc accatatttc gcctgtagcg ccacggcacg gcggtaaaac
     2881 tccagcccga gtagcgttgg ttcgccgccc tgccaggtaa aagccacttc gttttgcggt
     2941 tcgctggcag cgatatagtg acggacatac gcttccagcg tgtcatcgtc catatgcgtg
     3001 actggctttt cgcggtaaag ggattgtttt tcgagataaa aacagtagtc acagttcaga
     3061 ttacaatcgg aaccgctcgg tttcgccatc acatgaaaag cacgcgttgg aacctgttgc
     3121 agcatggtcg ctccttgcct gaagtcatcc ttcaatacgt taagggcgaa atcataccgt
     3181 ggggcaatga gttattacgg gcgtttattg cgaagggagg gtgacgaaat gcacagaaaa
     3241 caaaaaaccc cgccgaagcg gggttcaaaa ttggtcggcg agagaggatt cgaacctccg
     3301 acccactggt cccaaaccag ttgcgctacc aagctgcgct actcgccgaa atactgcttt
     3361 ttgaattttt agttcaattc tttaaagtcg tggtgcgagg ggggggactt gaacccccac
     3421 gtccgtaagg acactaacac ctgaagctag cgcgtctacc aattccgcca ccttcgcatt
     3481 gtcacaactt ctaataatgg ggtggctaat gggattcgaa cccacgacaa ctggaatcac
     3541 aatccagggc tctaccaact gagctatagc caccactaca aatcttgtta cgcggtatta
     3601 ctaccaccgc agctcaagcg ccgggactaa atggcgcgcc cgacaggatt cgaacctgag
     3661 acctctgcct ccggagggca gcgctctatc cagctgagct acgggcgctt agcgccgttg
     3721 cgggggtgga taatacggac ttcccccccc tctgtctagt ccctttttaa ataaattgcg
     3781 cgtttggtta cggtttgtgc gttttgctgc ttattcctcc agtttatgcg ctttcccgtg
     3841 gcgattaagg ccaaaaactt tataaatcgc cgtcaccgcc agcataaaga tgatgccaac
     3901 aaacagcgac atacgcgtat cttcattaaa gtacatgccg atcaaaacgc aaatcaggaa
     3961 tgccattgtt acgtagttgg cccacgggaa caggattgag cggaacggat ggctggcaat
     4021 cgccgcttta tgtgcacgcc gaaaacgcag ctggcttatc aatatcacaa accatggcac
     4081 catccccgga agcacgctgg cactgtagac gtagacaaac acacgctgcg gattgggaat
     4141 gatgtagttc aggcatgagc caattagcag aatagcaata gatactgcca cacccgcaac
     4201 cggtacgccg tgacgggaaa ctttcgccat cgccgccggt aactgacggt ttttcgccag
     4261 tgcgtagagc atacgtccgc aactgtacat gccgctgtta cagccagaga gcgcagccgt
     4321 cagcaccaca aagttgataa tgcccgccgc tgcggtaata ccgattttgg caaaagtcag
     4381 tacgaacggg ctgccgttgc tgcctatttc attccacggg aagatggtga cgataacgaa
     4441 aatcgcgcca acgtagaaaa tcaggatccg ccacagcacc ttgcctacgg cactgcgcag
     4501 cgtcacctgc ggattcttcg cttcaccggc agtaatgcca atcagctcca cgccctggta
     4561 ggacgccacc acaatacaca gcgcggtcag gaaccctttc cagccacccg caaagaaacc
     4621 gccatgctct gtgagattgc taaaaccaat cgactgcccg ccattgccaa agccaaagaa
     4681 aatcacgccc aggccaatga caatcatcac gataatcgtg gtgactttga tcatcgcgaa
     4741 ccagaactcg atttcgccgt acaaccgcac cgctgccaga ttcgccaacg ccaccagcgc
     4801 cactgcgatc aatgcgggta tccactgcgc catctccggg aaccagaact ggacataaac
     4861 gccaatggcg gtgatttcag agatccccac cgccatccac ataaaccagt aagaccaggc
     4921 ggtgagatag ccaaagaacg ggctcatata acgatgcgca taaacggcga acgaaccggt
     4981 aaccggttcg aggaacaaca tttcgcccat tgaacgcatg atgaaaaaga cgaacagccc
     5041 ggcgatgata taggccaaca atacggatgg cccggcccat ttcagggtac tggcggcccc
     5101 cataaacagg ccgacgccaa tggtgccccc cagggcgatg agttcgatat gtcgagcttc
     5161 cagcccacgc tgtagctctg gtttgttatc tgccataaat cctcgtgttg tgtttgcatg
     5221 ctttccggtg ttaccggtta tcgttatggg tacatcgagt gttgcgaatg ttttcgtaat
     5281 tcaggagaaa tggcaaataa agcattaaaa atttgaatgc tttgtgtaat aaaaaagcag
     5341 acaggcgacg gagtgaccac tccgtcgctt tacaaagaga ggaaaatcag aggttgccgg
     5401 tgtaatgcca gcgtaaataa cgcagcaaac gaagctgacg cttaatgcgg ctcggctgcg
     5461 aaagcaggcg gtagagccac tccagcccca gcgtttgcca gattttcggt gcgcgtttta
     5521 cgtgaccggt gaaaacatcg taagtcccgc caacgcccat atacagcgca tctggatgca
     5581 ccagacggca gtcgcgcatg aagatctcct gctttggcga tcccatcgca acggtgacga
     5641 tttgcgctcc gctggcatga atgcgttcaa acagcgcctg acgctgctcg ggtttaaaat
     5701 aaccatcctg actgccaacg atattcacat tccactggtt gcgcagttta gcttcagttt
     5761 gcgccagcac ttcaggttta ccgcccacaa gaaataccgt cgtcccttct ttgcctgcgc
     5821 gcgccatcag ctcttcccag agatcggcac cggcaacgcg ggaaacctgc gcctgcgggt
     5881 actttttacg tactgaacgt acaacgctga tgccatccgc atatttaaat tcggcagcgt
     5941 taattaactc cctgacctcg gcgttctctt caatggtcag cattttttca gcattaatgg
     6001 caaccagcgt tccctgctta agctgcccgt cagcaaacag ataatcgagg gcatgctgca
     6061 tatcacgcca accaatcaac tgtaagccac gcagcgtata ggtcggtgcc gtggtgttgt
     6121 tattcattgt tatccttcaa cctgcgtccg gagcgatgat tttgtacgtt tatgaatgag
     6181 tccggcgctt tcaaaaagcc agtacaacag ttttgcgatc atcagacatg cgccgaagac
     6241 cacgataaaa aagaccacgc gtgagacaaa cgaatccagc ccttcacgcg ccagcacgat
     6301 catattgaag atcgccccaa agcagaaact gtgcaatatc gcagccttat agcgattagt
     6361 ttcgcggttg cccagctcat acagccagtc gaaccatttg atgatcagtc caaccacaat
     6421 cgccccgagc gggatgaaca acgcgccgcc catcaccact agtgagccta taagcgtagg
     6481 cgagatcgcc agtccggagt ggttattcag cacttcccag gtaaagtagt tggctgagtt
     6541 cagcaccata ctcgggcgac ccggccacag ccaggaaggg ataaagacat agaaatcgcg
     6601 gacaattggc gccaggccct ggaagtcgat gttgtcgtag ttctgcaaca gcaacgccag
     6661 attctcccac ggcgagaagg tgtcgcgagt gagatagaga aacgtataga acgcttcatc
     6721 gccgctcaca ttcattccat agcgttttag cgccagccag aacatgccaa caatccccag
     6781 cacgcccgcc gccgccagca tccacaacga aatccagccg cgaataatgc caataaacag
     6841 gaagatagcg aatgcgatga tgatattggc gcgagtgccg ccgacaatca tataagtcag
     6901 caagccaaag gcgaccgtgc tgacgaggaa aaacagccac gctttgctgt cctggcgcag
     6961 aaagtagacc accagcatcg ccgggatgaa aaagtaaaag aagcgtttta acgccacgcc
     7021 ggagacttca ctggaaaaga tctgactgta ggagttaagc cggaacagca aaaagccgtt
     7081 gtgcatgaag aagatgccga cgcttaccag cgcgataccc atcaggatca cccacgtaag
     7141 attggtttcc acgcggttca tggtaaacag cggacggcgc ggtacatcag caacgcgttt
     7201 gcgtaggcgg gttttgtagg tgacgtagta aaccgcgtag aagcagcccg cagaaagcaa
     7261 cgcctgcaac aggatttctg gcggcgcgac accaacatca aagcgaaaca ccagcacgct
     7321 ggtcagcggg aagccaaaga aaaaggtgag cagaaacagc aatgaaaaga atacattgaa
     7381 gttaaagcgc acacgacgaa actcaaacca ggtcagcgtg gcaataaaca gcgtgcagag
     7441 cagccagaca acaaacaggc cactgaattg cagcagactc atgcgacctc cccggcggca
     7501 atcgccaacg cccgctgcca gccttgtaga tagttagggc taaagaaggc aatggtgttt
     7561 ttatccaccg acgccaactg gcgctgcgct tcacgcacaa tatcctcgtt gagatcgtcg
     7621 gtagtaaaca gcaccggtaa atgctgttcc gtcatatcct gccagaacgg attttcccgg
     7681 ttaagcacac aaggaatgcc cgcctgaatc agtaagcaca gcgtaccaat gccctgctgg
     7741 cgggcaaaaa taaagtaacc aagatcgcac tgacgaagta gcgtcagata ggcgtcaaat
     7801 tccagttttt cactcagaac ttgcagattt tcttcgctga ataactccag ccccgcctga
     7861 cgaacttcct cgatgtacgc ttcgttatga ggcggatatc ccatcggcac caccactttt
     7921 accgtatcgc caaattgctg atgaacggcg cgcaaggcag caatatgctc attgctgcgg
     7981 tcgccggagt tccccaccag aatggtcatt ttcccttcac gttgccgatc gttcgccatc
     8041 gtattgagag aagcatccat ccgcgtcggg aagtacagca gttcgccccg cacctttggg
     8101 tgcgttttgg caaaaaagct caaatcacca cgggtggcaa atacacagcc gactcgcttt
     8161 tgcgccaggc gacgtagtgg gtaaaaaagc ttatatctca agccactgga aagctcgtac
     8221 aggtctgccc cccagatatg ccagtaaaac tggctgggct taatgccacc actcagcaga
     8281 gccagccaca gtgtgggatt gaactgaccg tggaagaaaa aacgctgctg acggttagct
     8341 tttgctttcg cgatgaccgc ttccgccagc gattttttcc cagggaaaaa ttgcacagaa
     8401 agcgccggac agctatcact taagccgtcg tccttgccaa caaccataaa ctcgcgcgcg
     8461 tgctcgctcg tcgcggccag cgcgtcattg aaaaaccgca aaacggttcg gttatggtga
     8521 gggatatccg atcccagtac gtgaatcagt acagtcatgc ccgcctacgc cagagtaaaa
     8581 acacgccaca acaaagagaa aaatagacga tataggttgc catatatgcc tgtgccgcgc
     8641 ccagcgcgcc gtgcgtaggg attagccagt gggcaaatac catcaataaa gtgaactggc
     8701 tgacttccgc cagaatataa aaccgcagtg aagctttggc gatcaccaga taaccaaaga
     8761 cataagcgcc cacttttaac acatcaccca ccaactgcca ggcaaagaga tcgcgcatag
     8821 cggtaaattt attcgacaac agcagccaga tagcaaaatc acgcagcaac cagacggtaa
     8881 aactcgccgc cgccactgcc ggtaagacga atttcagcga tttaaccact tcccgggtga
     8941 tatcgcgctt ttccgttagc cgcgacaacg tgggcagcaa atatacgctg aacgatgccg
     9001 taataaattg caggtaggca tcggaaatac tgctcacccc ttgccagatc cccacctcat
     9061 cccagctata ctgcgccgcc agcagtttac gcatcatgat gtaagcaaca ggcaaggtca
     9121 ccgacgtaat caacgtcatg agcgtaaatt tgctcaactg ccctgccaga ccgttatccc
     9181 agctgggttt cagatagctt aacgggatag caccacgctt aattaacatg acggcggcag
     9241 gaatcaccac cagcgcggga atcaacgcca gacccagcaa cgccccttca tatccgccca
     9301 aacggtaact gacgtagtac gcaagaacgc caatcaagct gccaacaatc agagataacg
     9361 cattacctgc ggcatcgcga aagcctttca tcaacgccag taacaggttg ccccaggcga
     9421 tccccatttg caccagcgcc actaaacgca ccagcccctg atagtcgtga ttaccaaaca
     9481 atcccaagct gattggcgca gctgccagca caaaaaccag cgccatcagc gtagagaagc
     9541 caagtaccat cgctgatgaa gtgccgacca cgcggcgcag ctgttgcgga ttatcatggt
     9601 actgggcaac gtatttggtt acaccgttaa agatgccagc cccggcaagc acgccgagca
     9661 cggtaatcaa ctggcggaaa tttgccgcca gcccaagccc cgccggacca aatgacaccg
     9721 ccagcaactt accgaccagt aacccggcac caatcttgac cagtgtactg gccgccgtcc
     9781 acaaggacgc ttttgccaac gacatatcag gagaagtagt tcaacaaagt cgcaattacc
     9841 gtacgctgat tgacgggcga caggttgtag aacagcggca ggcgcagcag gcgctcgctc
     9901 tctttggtgg tgtagcgatc ttcaccgtgg aactcaccaa agcgttcccc cgcagggcaa
     9961 ccgtgcagcg gaatgtaatg gaacaccgcc atgatttccg cttctttcag aaagttaatc
    10021 aacgcgctcc ggtcaccaat atcccgcagt ttaatgtaaa acatatgcgc gttctgcaag
    10081 cagccatcgg gaatcgacgg cagctcgata cgcccggctt tcgccagagg tgctaacgca
    10141 tcgtagtagt tttgccacag cgccagacgt tgctggttga tacgatccgc tgcttccagt
    10201 tgcgcccaca ggtacgcagc ttgcagatcg gacatcaaat agctggagcc gatatcgcgc
    10261 caggtatatt tatcgacctg accacggaag aactggctgc ggtttgtgcc tttttcacgg
    10321 atgatctcgg ctcgttcgat caacgcttta tcgttaatca gcgtcgcgcc gccttcaccg
    10381 cccgccgtgt agtttttggt ttcatggaag ctaaagcagc caatatgacc aatggttccc
    10441 agtgcacgcc ctttgtaagt ggacatcacg ccctgagcag catcttccac cacaaacaga
    10501 ttatgctttt tcgccaacgc cataatggtg tccatttcgc aggccacgcc cgcgtaatgc
    10561 accggcacga taacgcgcgt tttgtcggtg atcgccgctt caatcaacgt ttcgtcgatg
    10621 ttcatggtgt ccgggcgaac atccacaaaa acgatttttg cgccacgcag cacaaaggca
    10681 ttggcggtgg agacaaaggt gtagctcggc atgatcactt catcgccagg ctggatatcg
    10741 agcagcagcg ccgccatctc cagcgaagcg gtgcaggacg gcgtcagtaa cactttggcg
    10801 ctgccaaaac gttgctccag ccactgctgg cagcgacggg taaaaccgcc atcgccacac
    10861 agtttgccgc tacccattgc cgactgcata tagtcgagtt cggttcccac caccggcggt
    10921 gcgttaaatg gaatcatgtg atcacctgta taaccagtac gcggtgcttt ctacattcgc
    10981 accactttgt atgtatcgtt taagcgcggc ggtgttgccc atttgggtcg ccacccgcaa
    11041 agttgtttta ccgcgagcat acgcccagtt tagcgccgtt tgcatcagct cagcacctgc
    11101 accgcgtcca gccagcaggc caattcgcgc atctgtagca ttgagttccc gtaaagagac
    11161 ataaccgcga atatcgccgg acgccgcacg taaaatcaga cattgatgat caaaggtgcc
    11221 gcgcacggca ttttcaatcc actgtgcata aaagcgactg ctggcgtcag gcgcatacca
    11281 cggcgcacga aaacggcttt gcgcaaatgc ggcgctggct aactgacgta atgcgggaat
    11341 atcggtctct tgtgccacta cagcaccgct atcactggca ttgttcacgg gtagcgccaa
    11401 atcaacttca ccttctacca gggagaatcc cagctgttgc agggcatcca gttcccccgt
    11461 atttgatgcc gcaattttgg cctgcacccg tgaccacggc gctaacgcgt ctggcgtcag
    11521 gaacggtgct tcagacgtaa tgcgcacgat ggcgctgtta acaccaaaga aggcgttttc
    11581 ccaggttagt ggctcaatac tggcgcggac gggcacgaag taactccagc agatattggc
    11641 cgtagccagt tttcgctaat gaactggcag cacgcttcac accctcgtca tcgagccagc
    11701 cgttacgcca ggcgatctct tccaggcagg caatcttaaa gccctggcgt ttttccaccg
    11761 tctgtacaaa ggtgctggct tcaatcaggc tgtcgtgagt gccagtgtcc agccaggcaa
    11821 atccgcgccc gagcagttca acggtcaggt tgcccgcctc gaggtacatc tggttgatgg
    11881 aggtaatctc cagttcacca cgctccgacg gcttcacctg ctttgcgtac tccacgactt
    11941 tactgtcgta gaaataaagc ccggtcaccg cccagtttga tttcggctgt tttggctttt
    12001 cttccagcga gatagcgcgg aaattatcgt caaactccac cacgccaaag cgttccgggt
    12061 ccatcacctg atagccaaaa accgtcgcca cttccgtgcg cgccgcaaca tgacgcagct
    12121 tcggactgaa gccctgaccg aagaagatgt tatcgcccag caccagacaa gaaggttcac
    12181 cattaaggaa ggtttcaccg atgataaagg cctgcgccag accgtccggg ctgggctgtt
    12241 cggcatattc cagctggata ccgaactcac taccatcgcc cagcaggcgc tggaaataac
    12301 ctttatcttc cggcgtagtg atgatgagaa tttcgcggat accggccagc atcagcaccg
    12361 acagcggata gtaaatcatt ggcttatcgt aaatcggcaa cagttgcttc gatacgccgc
    12421 gcgtaatcgg atgcaatcgg gtaccggaac cgcccgccag gataatacct ttcatgtttg
    12481 cctccgctga aaattagcct ttcagaccta aacgctcgcc ctgatagctg ccgtcctgca
    12541 cctgcttcct ccagctttca ttagccagat accactgcac cgttttacgc attccacttt
    12601 caaaggtttc ctgcggcagc cagccaagtt cacgggcaat tttcgaagca tcaatggcat
    12661 agcgcagatc atgccccgga cggtcagcga caaaggtgat caagtcacga taatgcacca
    12721 cgccttgcgg cttgttcgga gccagttctt ccagcagctc gcaaatggtt tccacaacat
    12781 cgagattctt acgctcgttg tgaccaccaa tattataggt ttcaccgact ttcccggtgg
    12841 tcgccacgca atacagcgcg cgggcgtgat cttcaacata cagccagtca cggatttgct
    12901 gcccgttgcc atataccggt agcggtttac ccgccagcgc gttgaggatc atcagcggga
    12961 tcagtttttc cggaaagtgg taaggaccgt agttattcga gcagttggtg ataagcgttg
    13021 gcagaccata ggttcgcagc caggcgcgca ccagatggtc gctgctggct tttgacgcgg
    13081 aataagggct gctcggcgca tacggcgtgg tttcggtgaa gaaatcatcc gtcgagtgca
    13141 ggtcaccata tacttcgtca gtggagatat gatgaaaacg gaacgctgat tttttatctt
    13201 ccgtcagcgc attccagtaa gcccgcgccg cttcaagcaa tgtataagtc ccgacaatgt
    13261 tggtttcaat aaacgctgcc gggccgtcaa tagaacggtc aacatggctt tcggctgcca
    13321 gatgcatgac acagtctggc tgatgctcag tgaatacgcg cgccagttct gcccgatcgc
    13381 agatatcaac tttctcaaag gcaaagcgct cgctttgcgc gaccggtgcc agcgacatca
    13441 ggtttccggc gtaggtcagc ttatcgacca ctaccaccgc gtcgctcgtt tcgttgatga
    13501 tataacgcac cagcgccgag ccaataaacc cggcaccgcc tgttatcaga atctttctca
    13561 tcagcgccag actcctttag catcgacgac atactgctga tggacattgt cgccattgat
    13621 aactttgaac tgactatgat cgaccagcat caccagcacg tctgccgttg ccagcgcctc
    13681 gtcaagctgc gccagagtac aaagcccggt cagttttttc ggcaactggt ggatgttagg
    13741 ctcaacaacc agagtttcgc cgctatgcca ctgggcgatc agttcagcga tttccatcgc
    13801 cgggctttcg cgcaggtcat caatattcgg tttaaacgcc agaccaaagc aggcgatttt
    13861 cagttcactg gcgcgtttat cggtagccgc caggcaatca gccaccgccg ctttcacctg
    13921 atcgataacc cagaacggtt tgtgatcgtt cacttcgcgc gcggtacgga taagccgcgc
    13981 ctgctggggg ttctgtgcca cgataaacca cggatcaaca gcaatgcagt gaccgcccac
    14041 gccagggcca ggctgaagaa tattaacgcg agggtgacga ttcgccaggc gaatcagttc
    14101 ccagacgtta atcccctgat cggcacaaat cagcgacaat tcattagcaa aagcgatatt
    14161 cacatcgcgg aagctgtttt cggtgagctt acacatttcc gccgtccgcg agttagtgac
    14221 gacacactca ccttcgagga aaattttgta cagttcgctg gcgcgggccg aacaaaccgg
    14281 cgtcatacca ccaatcacgc gatcgttttt aatcagctcg accataacct ggcccggtaa
    14341 cacgcgttcc gggcagtaag caatgttgac gtccgcctgc tcgcccacct gctgtgggaa
    14401 agtgagatcc ggacgcatct ctgctaacca ttctgccatc ttctcggttg accccaccgg
    14461 cgaggtggat tcaaggatca ccagcgcgcc ttttttcagc actggcgcaa tggagcgagc
    14521 agccgattca acgtaggtca tatctggctc atgatcgcct ttaaacggcg tgggtacagc
    14581 aatcagccag gcatccgctt caactggcgt cgtgctcgct cgtaaaaaac cgccttctac
    14641 ggcagttttt actacgctcg ccaaatcagg ctcgacgata tggatttcgc cacgattgat
    14701 ggtatcaacc gcgtgttggt tgatatcgac accaatcacc tgtttttgcc gtgaggcaaa
    14761 cgctgctgct gtaggcagcc cgatgtaacc cagtccgata acagaaatgg tcgcaaaact
    14821 catagtgata tccgattatt ttttaacgct tccagaatgc gagagcatgc ctgaccatca
    14881 ccatacgggt tatgggcgcg gctcatagtt tgatattcgt tttcgtcttt taaaagacgc
    14941 gtcacttcct cgacaattcg ctgcttatcc gtgcctacca gacgcaccgt acccgccgtc
    15001 accgcttccg gacgctcagt ggtatcgcgc atcaccagca ccggtttccc cagcgaaggc
    15061 gcttcttcct gaatgccgcc tgagtcggtc aaaatcagcc aggcgtggtt catcagccag
    15121 acaaacggta aatactcctg aggatcgatc agaatgacat ttttcacatg ccccagaatg
    15181 cgattgaccg gctctctgac gttcgggttg agatgcaccg gatagacaat ctggatgtcc
    15241 tggtgcgtgg tggcgatgtc tgccagcgcg tggcagattt cttcaaagcc acgaccgaag
    15301 ctttcacgcc tgtgaccggt caccagaatc atctttttat cggggtcgat aaacgggtaa
    15361 tttgccgcaa gttctgaacg cagcgtgtcg ctgctcatca cctggtcacg cacccataac
    15421 agtgcatcaa tgactgtatt accggtaatg aagattcggc tatctgcaac gttttcacgc
    15481 agcaagtttt gccgggaagt ttcggttgga gagaaatgat acatcgccag atgcccggtc
    15541 aatgtacggt tagcctcttc cggccacggc gaatagagat cgcccgtgcg taaaccagcc
    15601 tcaacgtgac caacaggaat acgctgataa aacgccgcca ggctggttgc cagcgtcgtc
    15661 gttgtgtcgc cgtgaaccag cacgacgtct ggtttgaact cggcaagaat aggttttagc
    15721 ccttccagaa tccgacaggt tatctctgtc aggccctgtc ctggctgcat tatgttgaga
    15781 tcgtagtcag gtacaatgga aaagagtttc agcacctgat cgagcatctc ccgatgctgc
    15841 gcagtgacgc aaactttagc ctcaaaaaaa ggatcttttg ccaacgcatg caccaacgga
    15901 gccatcttga tggcttccgg gcgcgtacca aatacagtca gtactttcac atcgattctc
    15961 ttcgaataag cggcgagcgc ctttgcgctc accgcagcag tgttgctatt tcgagcaacg
    16021 gcgggttaat gcgacaccag ccccgatcag ccccccgaca atgccccaca taatcatcag
    16081 gaaggcacga cgtgggctat cgcgttttac cggttcttcc ggcgtacgca aatagcgata
    16141 ggtctgaaaa cgcggatcca gggttggacc aacattcagg gtgtttaaca tggcccgatt
    16201 ctgatcatag tcgagatcaa aggccggacc gacggcctgt aaattttcca gtcgagcctg
    16261 gagcattgga cgcccaagca ggaacatttc tgaatcaggt aattcctcgg caggcacatc
    16321 tgtcgcactg cgcgaaatat tatgctgctc agcaattttc agcgcctgct caatgctgtt
    16381 catccggcgg tcgtagatgg ctttcgccac ctcttcctga cgcttcacct gtgctttcat
    16441 ctggatggta cgcgccgccc atgcgccttt cagctcatca ttcagatggc tggctgcacg
    16501 ctggctggca aaagcaacat actgacgtaa caggttatta gcgtcaggcg cggtttcggc
    16561 aataagcttc acgctgtcat tgaccgcgcg ggtaaagtct ccggggataa actggatgtt
    16621 gttaatcatt tcatccagca acgccgcatc ggctttgctg ttgcccacca tccgctgctt
    16681 gtaatagtcg gtttgcagcc agaactctct gcgggtatcc cacgaggcca gttgcataac
    16741 aaactcttta taggcttcgt ccatgaccga tggttggtcg gcagaagcca tgtttgaacg
    16801 gacatccagg ttacgcaaaa attgctgctg cgagtaatat ccccccagca tattcaccgt
    16861 tggacgatcg gtaatcgccg tcgagctcca ctcctgacga gcaaaaaacg tatacgccag
    16921 cgcgattaac gcaaacgcca gccccatgcc aataatccat agcttcccag cccacaaggt
    16981 acgaaacaac ccacgaatat ccagttcatt ttcagcgtct tcggccggtt tcccaggcat
    17041 tggttgtgtc atcacatcct catttatttg gttaaattgg ggctgccacc acgatttcta
    17101 cgcagtctgc gttttacgcg cttaataaag cgagcaactt tccaggcacg cttaatgcaa
    17161 tatccataga ggaggaatgc tagcaaaaag agcaccaaca tgacccactc cgggacaaaa
    17221 tgagaatatt ctgccagcac gccaatggaa gcgagcagtg ctgcggcaag ggtaatcagc
    17281 acaaacgcct ggcgggaagt aaacccggca cgcatgatca aatggtgaat atgctgacgg
    17341 tcaggagaga atgggctcat gcctttacgc aggcgacggt acataatcgc caccatatcc
    17401 attagcggaa tggcgattat ccacaaagcg gtaaccgggc tgatgggatg ggttttgcct
    17461 tgggtcgttt cgagcaggat ccagatcacg gtaaaaccaa tcagcgtact gcccgcatca
    17521 cccataaaga ctttgtagcg gcgacccagg ataccaaggt taagcatgat atatggcagg
    17581 atggcggcga tcatcgcaaa gcaccagatt gcgaggctgg tttgcccgtc gaaccacaaa
    17641 atcataccga ttgctgcaaa cgagacgcag gacaacccgc ccagcaagcc atcaatgcca
    17701 tcaaccatgt tgaacgcatt aatggccgcc cagacggcaa atagcgtcag gaagtaacca
    17761 aacggtccga gcaccatctc ccaggagcca aagatataac ccaggctact gagataaagg
    17821 ttgccaaaca ccatcataac aatgccaaca gcggcctgta tggtggcacg gatttttacg
    17881 ctgatatcaa aacggtcatc cagcgcgcca atgaaaacaa gcacaccggc acaagcgaga
    17941 tagagagatg catgcggaat atagtaatcg acaattccga acgtgaagca aatccctgcg
    18001 taaaccgaaa tacccccaac gagtggtatc aacccctggt gacgtttgcg gaagtttggt
    18061 ttatccacta aaccgacttt ttttgccacc ttacgggcaa aaaacagaaa cagtgtcgtg
    18121 aataaaaaaa tactgatgag atcagtactc actgtcagta aattcacaat gcatgctctc
    18181 agagaaaatt attagcagaa gtataaccac gaagaccttt attcagaagg gaaagtccgt
    18241 tccgaaccca ccagtcctgt gcaaaaatta accatttgat atgcaaattg cataacatgc
    18301 cagcaaattc caggaatttt cctaacatcg ccagcgcggc attaagatta cagatataaa
    18361 agcaaaacgc cacgtaaaca cgtggcgttt ttggcataag acaaatttat gagcgtttca
    18421 tcatttcgaa gaaatcgtca ttggtcttgg tcattgccag tttattaatg aggaattcca
    18481 ttgcatcgat ttcgcccatc gggtgaatga ttttgcgcag gatccacatt ttctgcagtt
    18541 cttcctgagt cgtgagcagc tcttctttac gggtaccgga acggttgtag tcgatagccg
    18601 ggaagacgcg tttttcagcg atcttacgag agaggtgcag ttccatgttg cctgtacctt
    18661 taaactcttc gtaaataact tcgtccattt tagaaccggt atcgataagc gccgtcgcga
    18721 taatggtcag gctgccgccc tcttccacgt tacgcgccgc accgaagaag cgtttcggac
    18781 gatgcagggc gttggcatcc acaccaccgg tcaacacttt acctgacgcc ggaacaacgg
    18841 tgttgtaagc gcgcgccaga cgagtgatgg agtcgagcag aatgataacg tctttcttgt
    18901 gctcaaccag gcgtttcgcc ttctcgatca ccatttccgc aacctgaacg tggcgagatg
    18961 cgggttcgtc aaaggtagaa gcaacaactt caccttttac cagtcgctgc atctcggtta
    19021 cttcttccgg acgttcgtcg atcagcagaa ccatcagcac acaatccggg tggttgtaag
    19081 caatgctctg agcaatgttc tgcagcagca tggttttacc ggctttcggc ggtgccacaa
    19141 tcagaccacg ctgaccacga ccgataggtg atgccagatc cagtacgcga gcggttaaat
    19201 cttcagtaga accgttacca cgttccatac gcagacgaga gtttgcgtgc agcggggtta
    19261 agttctcaaa gaggattttg ttgcgggcgt tttcaggttt gtcgaagtta acttcgttaa
    19321 ctttcagcag cgcaaaatag cgttcacctt ctttcggcgg gcgaatctta ccagagatgg
    19381 tatcaccagt gcggaggttg aaacggcgga tttggctagg ggaaacgtag atgtcatcag
    19441 gaccggcgag gtaggagctg tctgcggaac ggaggaaacc aaatccatcc tgcaatatct
    19501 ccagtacgcc atcaccaaag atatcttcgc cactctttgc gtgctgcttc aggatagcaa
    19561 aaataatgtc ctgcttacgc atacgagcca ggttttccag ccccatattt tcgccgagag
    19621 tgatcagctc agaaaccggc gtattcttta attcggtaag attcatagtg gtgtgagttc
    19681 ttaaacttgg ggtaaatctc gaacttaatg ttgtgaatgg tatggcagga tcatccatgc
    19741 ctgtttacgg tcatcgactc atgtcttttc gctgcctggt cacaggagag tacgcagaac
    19801 tgaaacgaca agacggattg agtgacgagc cagaaatctg ttcacttcgc atttaagata
    19861 aaacgggaag cgttggaaat tacaagattc aaacttaata aggtatgttt aatacgaagt
    19921 caacactaag ttagcatgac tcacgccggg cgtccagttt ttagcgacgg ggcacccgaa
    19981 catgaaattc ccttacgcca ggttagcgtc gaggaactct ttcaactgac ctttagacag
    20041 tgcgcccact ttggttgccg ccacttcacc gtttttgaac agcagcagag tcgggatacc
    20101 acggatgcca tatttcggcg cagtgccagg gttttgatcg atgttcagtt ttgcaacggt
    20161 cagtttgccc tgatattcgt cagcgatttc atccagaatc ggggcgatca ttttgcacgg
    20221 accgcaccac tctgcccaga aatcgacgag gatcgccccg tccgctttga gtacatccgt
    20281 gtcaaaactg tcgtcagtca ggtgaataat tttatcgctc atatataact ccacaggaat
    20341 aagcctggcg tgttggtttc gttgttggtg taacattaac caactaaagg ttgactttat
    20401 ttcaccggat acgctttcgt aaagcaatag taagctgata ttctaccaca ctatgagcaa
    20461 aacacattta acagaacaga agttttccga cttcgccctg catccgaagg ttgtagaagc
    20521 ccttgaaaaa aaagggtttc ataactgtac gcccattcag gcactggccc ttccgctgac
    20581 gctggcgggt cgtgacgtag ccgggcaggc gcaaaccggt accgggaaaa cgatggcgtt
    20641 tcttacgtca acgtttcatt gtcttctctc tcatcccgcg attgccgatc gcaaggtgaa
    20701 tcagccgcgt gccttaatta tggcaccgac gcgtgaactt gccgtgcaga tccatgccga
    20761 tgcggaaccg ctggcggaag ctaccggcct gaagctgggt ctggcttacg gtggtgatgg
    20821 ttacgacaaa cagctgaaag tgctggaaag cggcgttgac attctgattg gcaccacggg
    20881 gcgtttaatt gactacgcca agcagaacca cattaacctc ggtgccattc aggtagtggt
    20941 actggacgaa gccgatcgca tgtacgatct gggctttatt aaagatatcc gctggctgtt
    21001 ccgccgtatg ccgcctgcaa accagcgcct caacatgctg ttctccgcca cgctttcgta
    21061 ccgggtacgt gaactggcgt tcgagcagat gaacaatgcc gaatatattg aagtggaacc
    21121 ggaacagaaa acgggccacc gtattaaaga agagcttttc tacccttcta acgaagaaaa
    21181 aatgcgtttg ctgcaaacgc tgatcgaaga agagtggcca gacagagcga ttattttcgc
    21241 caataccaaa caccgttgtg aagagatctg gggccacctg gcggcagatg gtcatcgtgt
    21301 cggtttgttg acaggcgatg tcgcgcagaa aaaacgtctg cgtattcttg atgaatttac
    21361 ccgtggcgat ctggatattc tggttgccac cgacgttgcc gcgcgtggtt tgcatattcc
    21421 ggcagtgacg cacgtcttta actacgattt acccgatgac tgtgaagatt acgttcaccg
    21481 tattggtcgt acaggtcgcg caggcgcaag cggtcactct attagcctgg cgtgtgaaga
    21541 gtatgcattg aatttgcctg ctattgagac ctatattggt cactcaattc cggtaagcaa
    21601 atacaatccg gacgcattga tgaccgatct gccaaaaccg ctgcgcctca cgcgcccgcg
    21661 cacaggcaat ggtccgcgtc gtactggcgc tccgcgtaat cgtcgtcgtt caggttaaat
    21721 aaaaaatgtc atactcaaaa tcattcaaac tgtatcaaga tggcaagaga atgaatcccg
    21781 atgagcttac tgtagtaagt gattcaggtg aaagaatgca gccaacacag agacagattg
    21841 aaggatgaag agtatgggtt ccacctcatc gctgtatgca gccattgatc tcggttcgaa
    21901 tagttttcat atgctggttg tgcgcgaggt ggctggaagc atccagacgc tgacgcgaat
    21961 aaaacgcaaa gtgcgtctgg ctgctggcct gaatagcgaa aatgccctct ccaatgaagc
    22021 aatggagcgc ggttggcaat gtttgcgcct gtttgctgaa cgtctgcaag atattcctcc
    22081 ctcgcaaatt cgcgttgtcg ctacggcgac gttacgcctt gccgtcaatg cgggtgattt
    22141 tattgccaaa gcgcaggaaa tcctcggttg tccggtacag gtgatcagcg gtgaagagga
    22201 agcacgtctg atttatcagg gtgttgctca caccactggc ggtgccgatc agcgcctggt
    22261 agtggatatc ggcggtgcca gtactgaact ggtaaccggc acgggtgcac aaaccacctc
    22321 gttgttcagc ctgtcgatgg gctgcgtcac ctggctggaa cgctattttg ccgatcgtaa
    22381 tctggggcag gaaaattttg atgctgcaga aaaagcggca cgcgaagtgt tacgtccggt
    22441 tgccgatgaa ttacggtatc acggctggaa agtgtgcgtt ggtgcttccg gcaccgtgca
    22501 ggcgttacag gaaatcatga tggcacaggg gatggatgaa cgcattaccc tggaaaagtt
    22561 gcagcaattg aaacagcgag ccattcattg cggtcggctg gaggaactgg agattgacgg
    22621 gctgacgctg gaacgtgcgt tagtgttccc gagtggtctg gcgatcctga tcgccatttt
    22681 taccgaactg aatattcagt gtatgaccct ggcgggcggt gcgctgcgtg aaggcctggt
    22741 ctacggcatg ttgcatctgg ccgtcgagca ggatattcgc agccgtacgc tgcgtaatat
    22801 tcaacgccgc tttatgatcg atattgatca ggcacagcgc gtagccaaag ttgcggctaa
    22861 cttcttcgat caggtggaaa acgaatggca tcttgaagca ataagccgcg atttgctcat
    22921 cagcgcctgt cagcttcatg aaatcggcct gagcgttgac ttcaaacaag cgccgcaaca
    22981 cgctgcttat ctggtgcgta atctggatct tcccggtttt acccccgcac agaaaaaatt
    23041 gctggcgacg ctactgctca accagactaa tccggtcgat ctctcatcgc tgcatcagca
    23101 aaatgccgta ccgccgcgcg tcgcagaaca actctgccgt ttactacgcc tggccatcat
    23161 ttttgccagc cgtcgccgtg acgatctcgt gccagagatg acattacagg ctaaccatga
    23221 actgttgacc ttgacgcttc cgcaaggttg gctaacccaa catccgctgg gtaaagagat
    23281 tattgctcag gaaagccagt ggcagagcta tgtccactgg ccgctggaag tgcattaatg
    23341 agtaagtgcc ggatgcgatg ctgacgcatc ttttccggcc ttgattattt ccctcgtttt
    23401 gccgccatca tcgctttcag attcgccaga tggctttgcc ctttctgcat ccgttcttcg
    23461 gcgctgacca ctttgcgctc ctgttcccaa atcagatcat cctgcggcag ctccagcaaa
    23521 aagcggctcg gctccgggcg caccagttcg ccgtactgac ggcgttcttt acacagcgta
    23581 aaggtcaatt ccttctgggc gcgggtaatg ccgacatagg ccagccgccg ctcctcatcg
    23641 atattatctt catcgatact gctctggtgc ggcaaaaacc cttcttccat accgaccatg
    23701 tagacataag gaaactccag tccttttgac gcgtggagag tcatcagttg cacctgatcc
    23761 agctcttctt cactctcacc acgctccatc atgtcgcgca aagtaaagcg cgtcaccacc
    23821 tgggtgagcg tcatcggctc atccagttcg ctgccttcca gcatctccgt catccagcta
    23881 aacagttggt tgacgttctt catgcgcatt tcggcggctt tcgggctggg cgatgtttcg
    23941 tacagccagg attcataatc catgccatgg atcagatcac gcaccgcagc aatcggctcc
    24001 cgctccgcca ggcgctggat ttctgccaac cagtgagtga agcgggtcaa tgcttcataa
    24061 ccacgtccgc taagcgtctg actcaggccc atatcaaagc tggcggtaaa catgctttta
    24121 ttgcgcgtca tcgcccactc acccagcttt ttcagcgtag ccgggccaat ctctcgcttc
    24181 ggcgtgttaa cgatacgcag aaatgcgctg tcatcgtccg ggttagtcag cacgcgcaga
    24241 taagccagca agtccttgat ttcaggacga gagaaaaacg acgtaccacc ggatattttg
    24301 tacgggatgc ggttttgcat caggaatttt tcaaataccc gcgactgatg gttgccgcga
    24361 taaagaatgg cgtaatcttt gtactgcgtt ttattgacga agtgatgggc gatcagctcg
    24421 ccagtaacgc gctcggcttc atgttcctcg ttattcgcgc ttaaaacttt tagctctgtg
    24481 ccgtaaccca gttcggagaa cagacgcttt tcaaagacgt gcgggttatt ggcgatcagg
    24541 atgttcgccg ctttcagaat acgcccggaa gagcgatagt tctgctcaag cttaatcacc
    24601 ttcagcgccg gaaaatcctg actcagcagc accaggtttt gcggacgtgc accgcgccag
    24661 gagtagatcg actggtcatc gtcacccacc acggtaaagc gcgcgcggct gcccaccagc
    24721 agtttcacca gttcatactg gctggtgttg gtgtcctgat actcatccac cagcaggtag
    24781 cgaattttgt tctgccagcg ttcgcgtact tcttcattgc gttgcagcag caacgtcggc
    24841 aataaaatca ggtcatcgaa gtcgagaacg ttacaggctt tcaggtgtgc atcatacagc
    24901 ccataacaat gggcaaaaat acgatcccgc tcgccctttg cctctgccgc cgcctgcgcc
    24961 ggagttttga gatcgttctt ccagttagag atggtcgaaa tcagttgttg caggagaact
    25021 ttgtcatctt caatcagccc ctcggtcaac tctttaagca aagcaagctg atcggtatcg
    25081 tcaaataacg agaagttcgc tttcatccca agcgccgcat actcgcgttt gatgatatcc
    25141 agccccaacg tatggaaagt ggagatcatc agcccacgcg cctctttacg ccccagcgtc
    25201 tgccctacac gctctttcat ctcgcgcgct gctttattag taaaggtcac cgccgcaatg
    25261 tgccgcgcct gataaccgca accgcggatc agatgggcga ttttattggt gataacacga
    25321 gttttaccgg aacccgcgcc cgccagcacc aggcaggggc cggtaacgaa ttcgacagct
    25381 tgttgttggc cggggtttag acgcataggt gtattgctca atcttcgaac ggggggagga
    25441 ttgtagcaga atcgcatttg aatgtgaccg cgtgtaagcc gctaaccggt tggctgcgga
    25501 tgtttaaatc agagagagaa gaccgaaatc ggagttcatc gtgaatccat tagacttaga
    25561 aaatatcggg tcgtaacgtt gtgttattac tttttcagag tttactaagg agaggtttac
    25621 tcgtggcgat taatttcagc cctaaggttg gtgaaatact ggaatgcaac tttggtaact
    25681 atcctgttag ccaaaatgga ccgttcagca cgacgtatta cgatggccgc attcctcctg
    25741 aaatgataaa aaacaggtta gttgtcgtgc tcaacggaaa aattaatggc aacgcattca
    25801 tcgtcgttcc attatcaacc actcgcgacc atgacaagtt aaaacgtgga atgcatgttg
    25861 aaatagccag taatgttatc aatgacctgc aattctttga tcagcagatt cgttgggcca
    25921 aaaccgacct ggtacaacaa gtcagtcgaa acagattaaa cagagcaaga acttacagag
    25981 ggtatcttaa tcaatgtttg ccacatgagt tggttgcaga catacagagg gcggtcatca
    26041 agtcaatcaa tgccatatcg ctcataaatt gacaataaaa tcaccatccc cgataatgac
    26101 tttgtagccc atagaggccc taagttttct ccccttgagg gacctgcgaa gcccggattt
    26161 tccgggcttt tgccatatat gtaccagccc ctccctgact gacaattacg ttagcatcgg
    26221 cggatcatgc taaaatcgcc gcctgacaat tattcactca aggcacgatc atggcaaaaa
    26281 cagcagcagc actgcatatc cttgtaaaag aagagaaact ggctctggat cttctcgagc
    26341 agattaagaa cggggccgat tttggcaagc tggcgaagaa acactccatt tgcccatcag
    26401 gcaaacgcgg cggtgattta ggtgaattcc gccagggtca gatggttccg gcgttcgata
    26461 aagttgtgtt ctcttgtccg gtactggagc caaccggccc gctgcacacc cagttcggtt
    26521 accacatcat taaggtgctg taccgtaact aatagcaagg ccttctccgg gagaaggcct
    26581 tgaactctga atcgctatta acccgcaaca gcaatacgtt tcatatcagt catgtagccg
    26641 cgcagtttct tacctacctg ctcaatcgca tggctgcgaa tcgcttcgtt caaatcacgc
    26701 agttgcgcgt tatctaccgc accttccgga atagctttac ccaaatcgcc cggttgcagc
    26761 tctgccataa acggtttcag caacggcaca caagcgtaag agaacagata gttaccgtac
    26821 tcagcggtat cagagataac cacgttcatt tcgtacagac gcttacgggc gatggtgttg
    26881 gcaatcagcg gcagttcgtg cagtgattca tagtaagcag attcttcgat gatgccggaa
    26941 tcaaccatgg tttcaaacgc cagctcaacg cccgctttca ccatcgcgat catcagtacg
    27001 cctttatcga agtactcctg ctcgccgatt ttgccttcat actgcggcgc ggtttcaaac
    27061 gcggttttgc cggtctcttc acgccaggtc agcagtttct tatcatcgtt ggcccagtcc
    27121 gccatcatgc cggaggagaa ttcgccggag atgatgtcgt ccatatgttt ctggaacagc
    27181 ggtgccatga tctctttcag ctgttctgac aacgcgtaag cgcgcagttt cgccgggtta
    27241 gagagacggt ccatcatcag ggtgatgccg ccctgtttca gcgcttcggt gatggtttcc
    27301 cagccgaact gaatcagttt ttctgcgtat gccggatcgg tgccttcttc caccagcttg
    27361 tcgaagcaca gcagagagcc ggcctgcaac ataccgcaca gaatggtttg ctcgcccatc
    27421 aggtcagatt tcacttccgc aacgaaagag gattccagca cgcccgcacg atgaccaccg
    27481 gttgccgccg cccatgcttt agcgatcgcc atgccttcgc ctttcggatc gttttccggg
    27541 tgaacggcaa tcagcgtcgg tacgccgaat ccacgtttgt actcttcacg tacttcggtg
    27601 cctgggcatt tcggcgcaac cattactacg gtgatgtctt tacggatctg ctcgcccact
    27661 tcgacgatgt tgaaaccatg cgagtaaccc agcgccgcgc cgtctttcat cagtggctgt
    27721 acggtgtgca ctacatcaga gtgctgcttg tccggcgtca ggttaaccac cagatccgcc
    27781 tgtgggatca gttcttcgta agtacccact ttaaaaccat tttcggtcgc tttacgccag
    27841 gacgcgcgct tctcggcaat cgcttcttta cgcagagcgt aggagatatc gagaccagaa
    27901 tcacgcatgt tcaggccctg gttcagaccc tgtgcgccac agccgacgat gaccactttt
    27961 ttaccctgaa ggtagctcgc gccatcggcg aattcgtcgc ggcccataaa gcgacatttg
    28021 cccaactgtg ccagttgctg gcgcaggttc agtgtattga agtagttagc catggtgatt
    28081 cctcgtgatg ttgtgcttct tattgttcgg ttcgcgtttg caagattgaa ttcactatat
    28141 gacaggaaat ttattgcgga aattgatata ttcacaacat catattgcaa tttttgcaac
    28201 gtcaacatcg agggctgtcc ctgtggattt acgcgatctg aaaaccttcc tgcatctggc
    28261 ggaaagccgc cattttggcc gcagcgcgcg ggcgatgcac gttagcccat ccacgctctc
    28321 acggcagatt cagcgcctgg aagaagatct cggtcagccg ctgtttgtgc gcgataaccg
    28381 cacggtgacg ctgactgaag cgggcgaaga actgcgcgtt ttcgcccagc aaacgctgtt
    28441 gcagtatcag cagctgcgcc acaccatcga tcagcaaggg ccgtcgctct ctggcgaatt
    28501 acatatcttc tgctcggtga ccgctgccta cagccatctg ccgccgattc tggatcgctt
    28561 ccgcgcggaa cacccgtcgg tggagattaa actcactact ggtgatgcgg cagatgcaat
    28621 ggaaaaagtg gtcacgggag aagcggatct ggcgattgcc gggaaaccgg agacgctacc
    28681 cggcgctgta gcgttttcga tgctggagaa tctggcggta gtgctgattg cgcccgcgct
    28741 gccctgcccg gtgcgtaatc aggtctcggt agagaagccg gactggtcaa cggtaccgtt
    28801 tatcatggcc gatcaggggc cagtacgccg ccgcattgaa ctgtggtttc gacgcaataa
    28861 aatcagcaac ccaatgattt acgccacggt tggcgggcat gaagcgatgg tatcgatggt
    28921 ggcgctcggc tgtggtgtgg cgctgttgcc ggaagtggtg ctggaaaaca gcccggagcc
    28981 tgtacgcaac cgggtaatga ttttagatcg cagcgatgag aaaacgccgt ttgaactggg
    29041 cgtgtgtgca caaaaaaagc ggctgcatga gccgctgatt gaggcgttct ggaggatttt
    29101 gccgaaccac aaatgacgtt gtcgcgcgag taggcctgat aagcgaagcg ctatcaggca
    29161 tttttcccta acccgccaaa aagaacctga acgccgggtt attggtttcg tcgtggcaat
    29221 cgtagcccag ctcattcagc cgggtttcga aatccggttc atggtcgcca agttcgaacg
    29281 ccgccagtac gcgcccgtag tcggtgccat ggctgcgata gtggaacaaa gaaatgttcc
    29341 agtgcgtacc cagcgtgttg aggaagcgca gcagcgcgcc cggtgattcc gggaattcga
    29401 agctgtagag gcgttcctgc aacggatgtg acggacgccc gccgaccata tagcgcacat
    29461 gcaacttcgc catttcgtcg tcggagagat ccaccacgct gtagccgccg tcgttgagca
    29521 tctgcaaaat ttctttgcgc tcttcgaggc cgcggctcag gcgcacgccg acaaagatgc
    29581 aggcgttttt ggcatcggca aaacggtagt tgaactcggt gaccgatcgc ccgccaagca
    29641 gttggcagaa tttgaggaag ctgccttttt cttccggaat ggtcaccgcc aacaacgctt
    29701 cacgctgttc gcccagttcg cagcgttccg agacgtagcg caggccgtgg aagttcacgt
    29761 tggcaccgga aagaatatgc gccagccgtt cgccgcgaat gttgtgctgg gcgatatatt
    29821 ttttcattcc cgccagcgcc agcgcgccag agggttccgc caccgcgcgc acatcttcga
    29881 ataaatcctt catcgccgca cagatcgcat cactatcgac ggtgatgata tcgtcgagat
    29941 actcctggca caaacggaag gtttcgtcgc cgatgcgttt taccgcaacg ccttcagcaa
    30001 acagccccac gcgcggcaga tcaaccggat gacccgcatc cagcgctgct ttcaggcagg
    30061 cggagtcttc cgcttctacg gcgattactt tgatttgcgg catcagttgt ttgatcagca
    30121 ccgccacgcc agcagccaga ccgccgccgc cgactggcac aaatacgcgg tcgagatggg
    30181 cgtcctgctg gagcagttcc agcgccagcg tgccttgccc ggcaatcacc atcggatggt
    30241 cgaacggcgg cacccaggtg aacccctgct gctgtgacag ttcgatcgct ttggctttcg
    30301 cttcatcaaa gttcgcgccg tggagcagca cttcgccgcc gaagccgcgc accgcgtcga
    30361 ctttgatgtc ggcggtggcg gttggcataa cgatcagggc cttcacgcct aaccgcgcag
    30421 aagaaaacgc gacgccctgc gcgtggttac ccgcagaagc agtgatcacg ccgtgcgctt
    30481 tttgttcttc cgtcaggccc gccatcatgg cgtatgcgcc gcgcagctta aagctgtgca
    30541 ctggctggcg atcttcgcgc ttcaccagaa tcacgttatc aagacgagac gagagctttt
    30601 ccattttttg tagcggcgtg acctgcgccg cttcgtagac cggcgcgcgc agcaccgctc
    30661 ttaaatattc agcaccttcc ggggcaccgg acaggggttg cgagtcagcc attattaacc
    30721 ccccagtttc gatttatcgc gcaccgcgcc tttgtcggcg ctggttgcca ggctggcata
    30781 agcacgcagg gcaaaggaga tctgacgttc acgatttttc ggcgtccagg ctttgtcacc
    30841 ccgagcttcc tgcgcttcac gacgcgccgc cagttcggca tcgcttacct gtaactgaat
    30901 gccacggttc ggaatgtcga tggcgatcag gtcgccgtct tcaatcaggc caatgctgcc
    30961 gccgcttgcc gcttccggtg agacgtggcc gatagaaagg ccagaggtgc cgccagagaa
    31021 acgaccgtcg gtgatcagcg cacaggcttt accgagcccc attgatttca ggaagctggt
    31081 tgggtagagc atttcctgca tccccggacc accttttggg ccttcatagc gaattactac
    31141 cacatctccg gcgacaactt taccgccgag aatcgcttct accgcgtcgt cctggctttc
    31201 gtacactttc gccgggccgg tgaatttgag gatgctgtca tcgacgcccg cagttttaac
    31261 gatgcagccg ttttccgcga aattaccgta gagcaccgcc aggccgccgt ctttgctata
    31321 ggcgtgttcc agcgagcgga tacagccatt ggcgcgatcg tcgtccagcg tatcccaacg
    31381 gcaatcttgc gagaatgcct gggtggtacg gatacccgca gggccggcgc ggaacatatt
    31441 ttttaccgcg tcatcctggg tcagcataac gtcgtattgt tccagcgttt gcggcaacgt
    31501 caggccaagt acgtttttca catcacggtt cagtaacccc gcacgatcca gctcgccgag
    31561 aataccgata acaccaccag cacggtgaac atcttccata tggtattttt gggtgctcgg
    31621 cgcgactttg cataactgcg gaaccttgcg ggaaagctta tcgatatcac tcatggtgaa
    31681 gtcgatttcc gcttcctgcg ccgccgccag caggtgaagt acggtgttag tcgatccacc
    31741 catcgcgata tccagcgtca tggcattttc aaacgccgct ttgctagcaa tattgcgggg
    31801 cagtgcactt tcgtcgtttt gctcgtagta acgtttggtc aattcaacaa tgcgtttacc
    31861 agcattgagg aacagctgct tacggtcaga gtgggttgcc agcagcgagc cgttgcccgg
    31921 ctgcgacaga cccagcgctt cggtcaggca gttcattgag ttagcggtaa acatcccgga
    31981 gcaggaaccg caggttgggc atgcggaacg ttcaacctga tcgctctggg agtcagatac
    32041 tttcgggtct gcgccctgga tcatcgcatc aaccagatcg agcttgatga tctgatcgga
    32101 cagtttggtt ttcccggcct ccatcgggcc gccggaaaca aagatcaccg gaatattcag
    32161 acgcagggaa gccatcagca tccccggggt gattttgtca cagttagaga tgcagaccat
    32221 ggcgtcggcg cagtgggcgt tgaccatata ctcaacggaa tcagcgatca gttcgcgaga
    32281 tggcagtgaa taaagcatcc ccccgtggcc catggcaatc ccatcatcca ccgcaatggt
    32341 gttgaactct ttggcaacgc cgccagccgc ttcaatttgt tcggcgacca gtttaccgag
    32401 atcgcgcaga tggacgtgac ccggtacaaa ttgggtgaac gagttcacaa ccgcgataat
    32461 cggcttaccg aaatcggcgt cggtcattcc ggtggcgcgc cacagcgcac gagcacccgc
    32521 catattacga ccatgagtgg tggtggcgga acggtactta ggcatacttt atttactccc
    32581 agtgtctgtc tcgtaaatgg gacggtgcgt gccgtcccat tttttgtatt tattgattaa
    32641 cttgatctaa ccagccccat ttatcttcgg tttcgccggt aaagaggccg aagaaggctt
    32701 gctgaatgcg tttggtgacc gggccacaac ggccttcgcc aacctgaata ccgtctacac
    32761 tgcgcactgg cgtgatttct gccgccgtac cggacataaa gacttcatcc gccaggtaca
    32821 aggattcgcg cgacagcacc tgctcacgca cttcaattcc cagctctttc gccagtttga
    32881 tgatggcatc acgggtaata cccggcagcg cggaggaggt gaacggtggg gtgaacagca
    32941 caccatcttt cacttcaaac aggttttcgc ctgcgccttc agagatataa ccgttcacat
    33001 ccagcgcgat accttcctga taaccgtggc ggcgcgcttc gctacccacc agcagggaag
    33061 agagatagtt accaccggct tttgccgcag ttgggatggt gtttggtgct gcgcggttcc
    33121 aggaggaaac catcgcatcg atcccctgct ccagcgcttc tgcgcccaga tacgctcccc
    33181 acgggaaagc ggcgataatc acatcggttg agtatcccgc tggcgggttt acgcccatgc
    33241 caacatcacc aacgaagatc agcggacgga tataggcgct ggtgagattg tttttgcgga
    33301 tcacgtcacg gcaagcttcc atcagctcat caatgctctg cgaaaccggg aagcgataga
    33361 ttttggcgga gtcatgcaga cgctgcatat gctcacgatg gcggaataca accggtcctt
    33421 tgtgcgagtc gtagcaacgg atgccttcaa aaaccgaggt gccatagtgc agcgcgtgcg
    33481 acatcacatg caccttcgcg tcttcccagc gaaccatctc cccattgaac caaatgtaat
    33541 cagctttctt cgtggtcatt tttatattcc ttttgcgctc aggcgcggat ttgttgtgat
    33601 gtggttgtgc tctggcagat ggcaacgtgt gcgacgtcca ccagtttatt taactgacta
    33661 aacagtaagt cgaccgaccg tgggctggca acggtcaatt cgatatttat attttgtgca
    33721 tcgctggcgg cggccatatt cattgagcag acgtggaaac cacgatgacg caccacgcgt
    33781 aaaacacgtt ctaaggtttc cggattgaag cgagccgata cattgacctg atgttgcatc
    33841 atgataattt ctccaacatt tctgaattac tggcaccagg cggcaccagc ggccagacgt
    33901 tctcaagttc gtcgattgag acatgaagca ggtatggccc atcactgttc agcatggtgt
    33961 cgagtgccgc ttcaacctgg tctttacggg tgatgtgttg gccagggatg ccgaaggcgc
    34021 tggctaactt gaggaaatcg gggttatcgg taagggtggt ttcgctgtat cgttcctgga
    34081 aaaacagttg ctgccattgt cgaaccatcc ctaaccgttg gttatcgagt aagacgattt
    34141 tcaacggtaa ctgcttgcgt tttacggtgc ccagctcttg tacattcatc atgaaagagc
    34201 cgtcaccgga gatacagacg acggtatcgt ttggtcgcgc gacttgcgcg ccaaccgccg
    34261 ccggtaaacc aaaacccatg gtgcctaagc cgctggaggt aatgaaattt tccgggcgag
    34321 tgtgtgcgat gtgctgcgcg gcccacatct gatgctgccc cacatctgtg gtcacaacgc
    34381 aatccgcagg tttacgatcc gacagttgtt ttaacaacaa cggcgcgtag atagcgtcac
    34441 cgggatggtc gtaacgccag gcatgttcat cacgcagctg cgcgcagtat tgctgccagt
    34501 cattgatatt taatggctgc tgtaatgctg gtaacagcgc atttaaatca ccctgtaatg
    34561 ccacatgtgc ctgacgcagc ttgttcattt ctgccgggtc gatatccata tggataacac
    34621 tggcgtgtgg tgcgaaggtg ttcaatttgc cggtcacccg gtcatcaaaa cgtgcaccca
    34681 cggcgatcag caagtcgcac tcctgcaccg cgaagttcgc cgctttggtg ccatgcattc
    34741 ccagcatgcc cagatagtac ggataatctg cttcaactgc gcccagccct ttcagcgtgc
    34801 aggtggcagg catttttgtg gtagcgagaa attctcgtaa agcaggaact gcctgcgcca
    34861 tacccacgcc accaccaacg tacagcatcg gtttttgcgc ttttgccagc atctggcgcg
    34921 cttgctcaac ttcggcatgt gggaaagtca cttcgttttc aacggtggtg aaccacggtt
    34981 ccaggtcgcc gctggctaac tggatatctt ttgggatatc gaccagaacc ggaccaggac
    35041 gacctgagct ggcaacgtcg aatgcttcag ccataatgcg cggcaactct tccagcgact
    35101 gcaccagaaa gctgtgcttg gtacaggcta acgacaatcc caggacatcc acttcctgaa
    35161 atgcgtcagt accgataaac ggtgcggaca cttgaccggt gatggcaaca acagggatag
    35221 aatctaacag tgcgtccgca agcccggtta tcaggttggt tgcgcccgga ccagacgtgg
    35281 cgatacatac gccagttttg ccggtagcac gggcataacc gatagccgcc attgccgcac
    35341 cctgctcatg tcggcacagc aagtgctcca cgccgccgtc atacaatgca tcgtaaaccg
    35401 gcataattgc gccacccgga taaccgaaaa cggtgttcac accctgtgcc cgcaacgcat
    35461 gtaccaccca ctgtgcgcca ttcatagtta gttccccgtc ctgaatcttg ggaaacagaa
    35521 ttttgtgctg ttattcattg tctgctcctc ggttatgttt ttaaggtcaa aaaaaacccc
    35581 cggacctttc ggtgcggggg tcttagttcg ttaaggcttg atctctaagc ctttcctcgt
    35641 ccaagtgcag ccccgcacgg tgggataata atcaccacca cgctaatcac gaccaggcta
    35701 atcactcgta gaagggctgt cattttgtct tttcttgcat cttgttcgaa ggaatgccta
    35761 aagagttacc atagattttg cgaatagcac aagatatttt ttggccgttt tttatgatgg
    35821 ctttaataat ttcaatagaa attgttgttt tttaaaaaga aaatagtgag tgaaaaaaat
    35881 tcaatttcag tacgaatgtt gttccctccc tcgtagttca tcctttgtcc gctgca
//
LOCUS       DBFIFI010000027        34968 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.027, whole genome shotgun sequence.
ACCESSION   DBFIFI010000027 DBFIFI010000000
VERSION     DBFIFI010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34968
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.027"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            81..677
                     /locus_tag="N7B39_001297"
     CDS             81..677
                     /locus_tag="N7B39_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2421308.1"
                     /translation="MKKALLAAALVMASGSALAVDGGHIDFNGMVQSGTCKVGVVDTG
                     MHSVTTDGVVTLDTANVTDTFAEVSATAVGLLPKEFMISVECDPGAPKNAELTMGSAS
                     YANTSGTLNNNMNITVNGIAPAQNVNIAVHNMKNKAGAAEIKQVHMNNSSEVQELTLD
                     AEGKGQYVFNASYVKAPNSPAVTAGHVTTNALYTVAYK"
     gene            764..1489
                     /locus_tag="N7B39_001298"
     CDS             764..1489
                     /locus_tag="N7B39_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HGU2421309.1"
                     /translation="MFPLVKKTVSALFVSTLLASAPAFADIIISGTRIIYNADKKDVN
                     VRLENKGNRPLLIQNWLDTGDDNADPSQIKVPFTATPPVSRVEPKRGQTVKVMYTGAT
                     ALPKDRESVYWFNVLEVPPKPKDAEADKNLLQLAFRTRIKLFYRPSGLQGEPAEAPAK
                     ITWKLNNAQLQANNPTPYYVSFNEVKLESGGKTYNVNSSMVTPFSQASFSVTSLPGSV
                     SSGKVVFKAINDFGGNIDGSATF"
     gene            1531..4131
                     /locus_tag="N7B39_001299"
     CDS             1531..4131
                     /locus_tag="N7B39_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
                     /protein_id="HGU2421310.1"
                     /translation="MYQFTHQKSRIPKKTLLAACCALFYSSNGAAADTVEYDSSFLMG
                     TGASTIDVKRYAQGNPTPPGLYNVRVFVNGQATSSLEIPFVDIGENSAAACLTHKNLA
                     QLHIKQPEQPVTLLAREGEEEDCLDLAKSYEKADVCFDGSDQFLDLTIPQAYVLKSYG
                     GYVDPSLWESGINAATLAYTLNAYHTSSDNDNSDSVYGAFNSGINLGAWHFRARGNYN
                     WTTDNGSDFDFQDRYLQRDIPAIRSQIIMGDAYTTGETFDSVNVRGVRLYSDSRMLPS
                     ALASYAPTIRGVANSNAKVTVTQSGYKIYETTVPPGEFVIDDISPSGFGSELVVTIEE
                     ADGSKRTFTQPFSSVVQMQRPGVGRWDFSAGKVIDDSLRSEPNMGQASYYYGLNNLFT
                     GYTGIQFTDNNYLAGLLGVGINTSIGAFAVDVTHSRAEIPDDKTYQGQSYRVTWNKLF
                     QDTGTSFNLAAYRYSTQDYLGLHDALVLIDDAKHLSADEDKNTMQTYSRMKNQFTVSI
                     NQPLNIAYEDYGSLFISGSWTYYWAANNSRTEYNVGYSKSVSWGSFSVNLQRSWNEDG
                     EKDDAMYVSVSVPIENILGGKRKSSGFRNLNTQLNTDFDGSHQLNVNSSGNTENNLVN
                     YSVNAGYSLDKNAGDLASVGGYLNYESGLGGISASASATSDNSQQYSISTDGGFVLHS
                     GGLTFTNNSFSSNDTLVLINALGAKGARINNSNNEIDRWGYAVTSSVSPYRENRVGLN
                     IETLENDVELKSTSATTVPRSGSVVLTRFETDEGRSAVLNITAANGKSIPFAAEVYQG
                     EVMIGSMGQGGQAFVRGINDSGELIVRWYENNQTIDCKLHYQFPAQPQTQGSTNTLLL
                     NNLTCQVANH"
     gene            4145..4693
                     /locus_tag="N7B39_001300"
     CDS             4145..4693
                     /locus_tag="N7B39_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2421311.1"
                     /translation="MKRILLTSALIGLGLPAVGSATDLNVDFTATVLATTCTITIVED
                     GGPAVTGSNDEYSLTIPDVGLDKVATAAPEAQANFKLKASDCSNGYSKIFTTLTGTTV
                     SGKLIVNEATSGAAGVGMGIKRRDTADSTFFTPNNTDKFEWSADEKASGVPLTVALRE
                     TTAGAGRTGAFQAKATFNFTYE"
     gene            4708..5313
                     /locus_tag="N7B39_001301"
     CDS             4708..5313
                     /locus_tag="N7B39_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2421312.1"
                     /translation="MKLKVIATLIATVAVGVSFNSNFASASTTSASLTVNSNLTMGTC
                     SAQIMDNSNKVINEVVFGNVYISELGAKSKVQQFKIRFSNCSGLPQNSAQIVLAPNGI
                     SCAGSQSSSAGFSNKFTDASAATRTAVEVWTTDTPESNGSTQFHCAQKIPVPVTLPAD
                     TTTQPYDYPLSARMTVAEGRLVTDVRPGNFRSPTTFTITYQ"
     gene            5334..5924
                     /locus_tag="N7B39_001302"
     CDS             5334..5924
                     /locus_tag="N7B39_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HGU2421313.1"
                     /translation="MLCRHHKIVHFLGLATALITPFAYSGQDVDLTAKIVPSTCQVEV
                     SNNGVVDLGTVTLDYFADNVTPTTDYAGGKTFNVNVVSCDNIQTTQSQMKLDFQPQAG
                     SLAQANNQIFSNEYEQQATGAKNVGIVIFSAQPNQQTFNVRGTDGSSTAIYSVAPGNA
                     VPSTWTFYSRMQRVNNALPPESGMVRSQVIVNVSYE"
     gene            5936..7045
                     /locus_tag="N7B39_001303"
     CDS             5936..7045
                     /locus_tag="N7B39_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2421314.1"
                     /translation="MQRKGNKLLIQLCSVILLFFTTSWYALANECYIERNAEGDYHMK
                     ISSTQLSLASQMVEVPTEIAEATWDVNIQLRGDAIGCKSLGDSKAVHFLNTADPSLIS
                     TYTTTNGAALLKTTVPGIVYSVELLCLSCGAADELDLWLPAQSGADNFIPSTQTKWAY
                     EYSDQSWYLRFRLFITPEFKPKNGVSSGTTIAGKIASWYIGTNDQPWINFYIDNDSLK
                     FFVDEPTCATVALAQDQGNVSGNQVTLGNSYVSEVKNGLTREIPFSIRAEYCYASKIT
                     VKLKAANKPSDATLVGKTTGSASGVAVKVNSTYDNSKVLLKADGSNTVDYNFAAWSNN
                     LLFLPFTAQLVPDGSGNAVGVGTFSGNATFSFTYE"
     gene            7180..7974
                     /gene="panB"
                     /locus_tag="N7B39_001304"
     CDS             7180..7974
                     /gene="panB"
                     /locus_tag="N7B39_001304"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HGU2421315.1"
                     /translation="MKPTTIASLQKCKQEKKRFATITAYDYSFAKLFAEEGLNVMLVG
                     DSLGMTVQGHESTLPVTVEDIAYHTTAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            7986..8837
                     /gene="panC"
                     /locus_tag="N7B39_001305"
     CDS             7986..8837
                     /gene="panC"
                     /locus_tag="N7B39_001305"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HGU2421316.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(9136..10032)
                     /gene="rpnC"
                     /locus_tag="N7B39_001306"
     CDS             complement(9136..10032)
                     /gene="rpnC"
                     /locus_tag="N7B39_001306"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HGU2421317.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSESQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFAQRFLSKG
                     MSREDVAEMTNLSLAEIDRLIN"
     gene            10306..10686
                     /gene="panD"
                     /locus_tag="N7B39_001307"
     CDS             10306..10686
                     /gene="panD"
                     /locus_tag="N7B39_001307"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HGU2421318.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(10690..11919)
                     /gene="yadE"
                     /locus_tag="N7B39_001308"
     CDS             complement(10690..11919)
                     /gene="yadE"
                     /locus_tag="N7B39_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HGU2421319.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFDKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMVQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFIVTSRIKRHPQKW
                     EPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(11983..12423)
                     /gene="yadI"
                     /locus_tag="N7B39_001309"
     CDS             complement(11983..12423)
                     /gene="yadI"
                     /locus_tag="N7B39_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGU2421320.1"
                     /translation="MLGWVITCHDDRAQEILDVLEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGAPEVSSIWHQQQKNPPFVLKHNLYEY"
     gene            complement(12528..13298)
                     /gene="yadH"
                     /locus_tag="N7B39_001310"
     CDS             complement(12528..13298)
                     /gene="yadH"
                     /locus_tag="N7B39_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU2421321.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(13295..14221)
                     /gene="yadG"
                     /locus_tag="N7B39_001311"
     CDS             complement(13295..14221)
                     /gene="yadG"
                     /locus_tag="N7B39_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU2421322.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            14330..14992
                     /gene="can"
                     /locus_tag="N7B39_001312"
     CDS             14330..14992
                     /gene="can"
                     /locus_tag="N7B39_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HGU2421323.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLDEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(15033..15569)
                     /gene="hpt"
                     /locus_tag="N7B39_001313"
     CDS             complement(15033..15569)
                     /gene="hpt"
                     /locus_tag="N7B39_001313"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HGU2421324.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            15775..18165
                     /gene="gcd"
                     /locus_tag="N7B39_001314"
     CDS             15775..18165
                     /gene="gcd"
                     /locus_tag="N7B39_001314"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HGU2421325.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(18367..19917)
                     /gene="cueO"
                     /locus_tag="N7B39_001315"
     CDS             complement(18367..19917)
                     /gene="cueO"
                     /locus_tag="N7B39_001315"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HGU2421326.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVNELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHDAPKERAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            20083..20430
                     /gene="yacC"
                     /locus_tag="N7B39_001316"
     CDS             20083..20430
                     /gene="yacC"
                     /locus_tag="N7B39_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HGU2421327.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            20536..21402
                     /gene="speE"
                     /locus_tag="N7B39_001317"
     CDS             20536..21402
                     /gene="speE"
                     /locus_tag="N7B39_001317"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HGU2421328.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAVHTAAFALP
                     QYLQDALASQPS"
     gene            21418..22212
                     /gene="speD"
                     /locus_tag="N7B39_001318"
     CDS             21418..22212
                     /gene="speD"
                     /locus_tag="N7B39_001318"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HGU2421329.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(22250..22612)
                     /gene="yacL"
                     /locus_tag="N7B39_001319"
     CDS             complement(22250..22612)
                     /gene="yacL"
                     /locus_tag="N7B39_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HGU2421330.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(22788..25385)
                     /gene="acnB"
                     /locus_tag="N7B39_001320"
     CDS             complement(22788..25385)
                     /gene="acnB"
                     /locus_tag="N7B39_001320"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HGU2421331.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAVAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            25740..27593
                     /gene="yacH"
                     /locus_tag="N7B39_001321"
     CDS             25740..27593
                     /gene="yacH"
                     /locus_tag="N7B39_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HGU2421332.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVETQSTQ
                     LAVSDAAAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDALLSQVLMASTY
                     PTNVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKTVPVTQTVTAPVIPSN
                     TVSTANPVITEPATTVISIEPGNPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDNYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVHEKMQTNPQNQQRREAARERIQPASPEQRQ
                     AVREKMQTNPQIQQRRDAARERIQSASPEQRQVFKEKVQQRPLNQQQRDNARQRVQSA
                     SPEQRQVFREKAQESRPQRLNDSNHTARLNNEQRSAVRERLSERGARRLER"
     gene            complement(27664..29088)
                     /gene="lpdA"
                     /locus_tag="N7B39_001322"
     CDS             complement(27664..29088)
                     /gene="lpdA"
                     /locus_tag="N7B39_001322"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HGU2421333.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(29296..31188)
                     /gene="aceF"
                     /locus_tag="N7B39_001323"
     CDS             complement(29296..31188)
                     /gene="aceF"
                     /locus_tag="N7B39_001323"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HGU2421334.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAVPEA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNTGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(31203..33866)
                     /gene="aceE"
                     /locus_tag="N7B39_001324"
     CDS             complement(31203..33866)
                     /gene="aceE"
                     /locus_tag="N7B39_001324"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HGU2421335.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(34027..34791)
                     /gene="pdhR"
                     /locus_tag="N7B39_001325"
     CDS             complement(34027..34791)
                     /gene="pdhR"
                     /locus_tag="N7B39_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HGU2421336.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
BASE COUNT         8641 a         9238 c         8571 g         8518 t
ORIGIN      
        1 ccctattaca tcctccaaaa actttgagta atatccgctc gcaatattaa tttcatataa
       61 tttttgtaag gatgcatgta atgaaaaaag cacttctcgc agccgctctg gttatggctt
      121 ctggttccgc cctggctgta gatggtggtc atatcgactt taacggtatg gtacagtccg
      181 gtacctgtaa agtgggtgtg gtagatactg gtatgcatag cgttaccact gatggcgtgg
      241 ttaccctgga tactgcgaat gttactgata cttttgctga agttagcgca actgctgtcg
      301 gtttactgcc gaaagagttc atgatttctg ttgagtgtga tccaggtgct ccgaagaatg
      361 ctgagttaac tatgggttct gcaagttacg cgaacaccag cggtaccctg aataacaata
      421 tgaacatcac tgttaacggt attgcaccgg ctcagaacgt aaacattgca gttcataaca
      481 tgaaaaacaa agctggcgct gctgaaatta agcaggtcca tatgaacaac tcttctgaag
      541 ttcaggaact gacattagac gcagaaggta aaggccagta cgtatttaac gcatcttacg
      601 ttaaagcacc gaacagcccg gctgtaactg ctggtcatgt aaccactaac gcgctgtaca
      661 ccgttgctta taagtaattg atggtgtgtt gagcataagg tttgccagca ggcaaacctt
      721 attttcgcga atttaatctt tctacagaca gcaaggagtg cttatgtttc ccttggttaa
      781 aaaaacggtt agtgcattat ttgtgagtac tctgttagcc agtgcaccag cattcgctga
      841 cattattatt tccggaacac gtatcattta taacgctgat aaaaaagatg ttaacgttcg
      901 tctggaaaat aaagggaatc gccccttact gattcagaac tggttggata cgggcgatga
      961 caatgccgat cccagccaga tcaaagtgcc gtttaccgcg accccaccag tttcacgcgt
     1021 ggagccaaaa cgcggtcaaa ccgtaaaagt gatgtatacc ggtgcaacag ccctgccgaa
     1081 agaccgtgaa agcgtctact ggtttaacgt gctggaagtg ccgcctaagc cgaaagatgc
     1141 tgaggctgac aaaaacttgt tacagctggc tttccgcaca cgcatcaaac tcttttatcg
     1201 cccgagtggg ttacagggag aaccggctga agcaccggcg aaaatcacct ggaaattaaa
     1261 caacgctcag ttacaggcaa ataacccaac gccatattac gtgtccttta atgaggtcaa
     1321 gctggaaagc ggcggcaaaa cctacaacgt caattccagc atggtcacgc cgttcagcca
     1381 ggcaagcttt agcgtaacca gtctgccagg aagcgtgtca tccggaaaag tggtctttaa
     1441 agcgatcaac gactttggcg gcaacattga tggttccgca acattctaaa aaataaacgg
     1501 attcttttta acatcgccag gaatgcaacc ttgtaccagt ttactcatca aaaaagccgt
     1561 atcccgaaaa aaacgctact tgcggcctgt tgtgccctgt tttatagcag caacggtgct
     1621 gcggcggaca ccgtggaata tgacagttcc tttttaatgg gaactggcgc atcaacgatt
     1681 gatgttaaac gttatgctca aggcaacccg acaccgccgg gtctctataa tgtccgcgta
     1741 tttgtaaacg gtcaggcgac ttccagctta gaaattccgt ttgtggatat tggcgaaaac
     1801 agtgcggcgg cctgtcttac ccataaaaac ctggcgcaac ttcacattaa gcaacctgaa
     1861 cagcctgtca ctttactcgc cagagaaggt gaagaagagg attgtctgga tctggcaaag
     1921 tcatacgaaa aggcggatgt gtgctttgac ggtagtgacc agtttctcga tctgacgatc
     1981 cctcaggcct atgttctgaa aagctatggc ggctacgttg acccttcttt atgggaatcg
     2041 ggaattaacg ctgccacact ggcatatacc ctgaacgcgt atcacacaag ttcagataac
     2101 gacaatagtg acagcgtcta tggcgcgttc aactcaggta tcaatttagg agcctggcac
     2161 tttcgtgcgc gcggtaacta taactggaca acagataacg gcagcgattt cgatttccag
     2221 gatcgttact tacagcgtga cattccggca atccgttccc agataattat gggtgatgcc
     2281 tataccaccg gtgaaacgtt tgactctgtc aacgtccgtg gtgttcgcct gtacagcgac
     2341 agccgtatgc tgccttcggc gctggccagt tacgctccga ccatccgcgg tgtagcaaac
     2401 tccaacgcca aagtcaccgt gacgcaaagc ggatataaaa tttatgaaac caccgttccg
     2461 cccggtgaat ttgttataga cgacattagc ccttccggct ttggtagcga actggtcgtg
     2521 accattgaag aagcggatgg ttccaaacgc acctttacgc aacccttctc gtcggttgta
     2581 caaatgcaac gtcctggtgt gggccgttgg gatttcagcg cgggtaaagt cattgatgac
     2641 agtctgcgat ccgaacccaa tatggggcaa gcctcttatt actatggtct gaataacctc
     2701 ttcacgggtt ataccggcat tcagttcacc gataataact atcttgccgg gctgttaggt
     2761 gtgggtatca acaccagcat cggcgccttt gcggtagacg ttacccattc ccgtgctgaa
     2821 attccggatg ataaaaccta ccaggggcaa agttatcgcg tgacctggaa caaacttttc
     2881 caggataccg ggacatcatt taacctcgcg gcgtaccgct attccaccca ggattacctg
     2941 ggcctgcatg atgcgttagt cctcattgac gacgccaagc atttgtctgc cgatgaagac
     3001 aaaaacacca tgcagacgta ctcacgtatg aaaaaccagt ttaccgtcag cattaaccag
     3061 ccattgaata tcgcctatga agattacggt tcgctgttta tttccggtag ctggacgtat
     3121 tactgggcgg cgaacaatag ccgcactgaa tataatgttg gttacagtaa aagcgtttcg
     3181 tggggcagtt tcagcgtcaa cctacaacgt agctggaatg aagacggcga gaaagatgac
     3241 gcgatgtacg tcagcgttag cgtacctatt gagaatattt taggtggcaa acgtaagtct
     3301 tctggtttcc gcaatttaaa tactcagctc aataccgatt tcgatggttc acatcagttg
     3361 aatgttaaca gttccggtaa cactgaaaac aatctggtga actacagtgt caacgcaggt
     3421 tatagcctcg ataaaaacgc cggcgattta gcctctgttg gtggttatct caactatgaa
     3481 tctgggttag gcggtatttc cgcttcggcc tcggccactt ctgataacag ccaacagtac
     3541 tccatctcaa ccgatggcgg ctttgtatta cacagtggtg gtttaacgtt cactaacaac
     3601 agtttcagca gtaacgacac gctggtgtta atcaacgccc taggtgctaa aggcgcacga
     3661 atcaataaca gtaataacga aatcgatcgc tggggatatg ccgtgacgtc ctctgtcagc
     3721 ccatatcgtg aaaaccgggt aggtctgaac attgaaacac tggaaaacga tgttgaactg
     3781 aaaagtacca gcgccaccac cgtaccacgt agcggctccg ttgttttgac ccgtttcgaa
     3841 actgacgagg ggcgttctgc cgtgctgaat attactgccg ccaatggcaa atccattccg
     3901 tttgctgcgg aggtttacca gggtgaggtg atgatcggca gcatgggcca gggtggtcag
     3961 gcatttgtac gcggtattaa cgacagcggg gaattaatcg tgcgctggta tgaaaacaac
     4021 caaaccattg actgtaagtt gcactaccag ttcccggcgc agccacaaac gcagggaagc
     4081 accaacacct tattacttaa caatcttacc tgtcaggtag caaatcacta atatggaaaa
     4141 ttctatgaaa aggatactac taacatccgc gttaataggc ctgggtttac cggcggttgg
     4201 ctctgcaaca gatcttaatg tcgattttac ggccaccgta ttagccacca cctgcacgat
     4261 aactatagtg gaagatggtg gtccagcagt gaccggtagt aacgatgaat acagcctgac
     4321 aattcctgat gttggtctgg ataaagtcgc taccgctgcg cccgaagctc aggccaactt
     4381 taaattaaag gcctccgatt gcagcaatgg ttactcaaag atttttacca cacttacagg
     4441 aacaacggtt tccggtaagt taattgttaa tgaagcaacc tctggcgctg ctggggtggg
     4501 gatggggatt aagcgtagag ataccgcaga ctcgactttc ttcacaccca ataatactga
     4561 caaatttgaa tggagcgccg atgagaaagc cagcggtgtg cctctgaccg tagcactgcg
     4621 cgaaacaacg gcgggagcag gacgcaccgg tgcgtttcag gcaaaagcca cctttaactt
     4681 cacctacgaa taacagggac agccaccatg aaattaaaag tcatcgctac actgattgct
     4741 actgttgccg tgggtgtaag ctttaacagc aattttgctt ctgcgagtac aacgtccgct
     4801 tctttaaccg taaacagtaa cctgactatg ggtacctgca gtgctcagat aatggataat
     4861 agtaataaag tgatcaatga agtggtcttt ggcaatgttt atatttctga actcggtgca
     4921 aaaagcaaag tgcaacagtt taaaattcgc tttagcaatt gctctggcct tccccaaaac
     4981 agcgcccaaa tagtgctggc acctaatggt atatcctgtg ctggttctca atcgtcatcg
     5041 gcgggttttt ctaacaagtt tactgacgct agcgcagcaa ccagaacggc tgtggaagta
     5101 tggactacag atacaccgga aagcaatggc agtacgcaat tccattgtgc tcaaaagata
     5161 ccagtgcctg tgacgcttcc cgccgacacc acaactcagc cttacgatta cccgttaagt
     5221 gcacggatga ccgttgcgga aggtagattg gtaaccgatg taagaccggg taatttccgc
     5281 tctcccacga ctttcacgat cacttatcag taatagcgga taaggatatt agaatgctat
     5341 gcaggcatca taaaatcgta cactttttag ggctggcaac agcccttatc acgccttttg
     5401 cctattccgg ccaggatgtg gatctcaccg caaagatcgt gcccagcacc tgtcaggttg
     5461 aagtcagtaa taatggcgtt gtcgatctcg gcacagtgac gctggactat tttgccgaca
     5521 atgtaacccc gacaacggat tatgcgggtg gcaaaacgtt taatgtgaat gtggtctctt
     5581 gcgataacat tcaaacgaca cagtcgcaaa tgaaattaga ttttcagccg caggcgggat
     5641 cgcttgcaca ggcgaataat caaattttta gtaatgaata tgaacagcag gctaccgggg
     5701 cgaaaaacgt cggcattgtg attttttcgg cccaaccgaa tcagcagaca tttaacgtgc
     5761 gaggaacgga tggttcctca acagcaattt attcggtagc acctggcaat gctgttccct
     5821 caacctggac cttttattcc cgtatgcagc gggtgaataa tgcccttcct cctgaatcgg
     5881 gaatggtgag aagccaggtt attgttaacg tatcttacga ataataagga taattatgca
     5941 aaggaaaggc aataaactgt tgattcagtt atgcagtgtg atactgctat tttttaccac
     6001 atcctggtat gcattggcga atgaatgtta tatagagaga aatgctgaag gggattatca
     6061 catgaagata agctctactc agcttagtct ggcgtcacaa atggtcgagg ttccgacaga
     6121 aatagccgaa gctacatggg atgtaaatat tcaactaaga ggcgatgcca tagggtgtaa
     6181 atctcttggg gatagtaagg cagttcactt tcttaataca gctgacccaa gtttaatatc
     6241 cacgtacacc acaacgaatg gcgcagcgtt attaaaaaca actgttccag gcattgtgta
     6301 ttctgtcgag ttattatgcc ttagttgtgg tgccgcagat gaacttgatt tatggctacc
     6361 tgcacaaagt ggcgcagata acttcatacc aagcacccag acgaaatggg cctatgagta
     6421 cagtgatcaa agttggtatt tacgttttcg cttattcata actcctgaat ttaaacccaa
     6481 gaatggtgtt tccagcggaa caacgatagc aggaaagatt gcgtcatggt atataggtac
     6541 caatgaccag ccgtggatca acttttacat tgacaatgac tctttaaagt ttttcgtcga
     6601 tgaaccgacc tgtgcaacag ttgccctggc acaagatcag ggcaacgtca gtggcaatca
     6661 ggtaacgctt gggaacagct atgtttcgga agtgaaaaat gggcttacgc gggaaatccc
     6721 tttttctatc cgtgctgaat actgttatgc cagtaaaatt acggttaagt tgaaagcggc
     6781 aaataaaccc agcgatgcca cactggtggg taaaacgact ggctcggctt caggcgtggc
     6841 tgtaaaagta aattcaactt atgacaatag caaagtattg ttaaaagcag atggtagcaa
     6901 cacggttgac tacaacttcg ccgcctggtc aaacaacctg ctgtttttac cttttacggc
     6961 gcagctggta ccggatggta gcggtaatgc tgtcggtgtt ggaacatttt caggtaacgc
     7021 gaccttctcc tttacctacg aataagtaaa acgtcattcc ctcctcatcg caccagagtt
     7081 ctgctctggt gcgaacattt ccccacacta ataaccattg cctccctgtt ttcggctgtt
     7141 agaatgcgat caatctgact ttagccatca ggatacgtta tgaaaccgac caccattgct
     7201 tcattgcaga aatgtaagca ggaaaaaaaa cgcttcgcca ccatcaccgc ctacgactac
     7261 agcttcgcaa aactgtttgc cgaggaaggg ctgaacgtga tgctggtggg cgactcgctg
     7321 gggatgaccg ttcaggggca tgaatctacc cttcccgtta ccgttgaaga tatcgcctac
     7381 cacactaccg cagtacgtcg tggcgcgcca aactgcctgc tgttggctga cctgccgttt
     7441 atggcgtatg ccacgccgga acaagccttt gaaaacgccg caacggttat gcgtgccggt
     7501 gctaacatgg tcaaaattga aggcggtgag tggctggtcg aaaccgtaaa aatgctgacc
     7561 gaacgtgccg ttcctgtatg tggtcactta ggtttaaccc cacagtcagt gaatattttc
     7621 ggtggctaca aagttcaggg gcgcggcgat gaagcgagcg atcgactgct tagcgatgca
     7681 ttagccttag aagccgctgg tgcacagttg ctggtgctgg aatgcgtgcc ggttgaactg
     7741 gcaaaacgta ttaccgaagc gctggcgatc ccggttatcg gtattggcgc aggcaacgtt
     7801 accgatgggc agatcctcgt gatgcacgat gcgttcggca ttaccggcgg tcatatccct
     7861 aaatttgcca aaaatttcct cgctgaaacg ggcgacatcc gcgctgcggt gcggcagtat
     7921 atggctgaag tggagtccgg cgtttatccg ggcgaagaac acagtttcca ttaaggagtg
     7981 acgttgtgtt aattatcgaa accctgccac tgctgcgcca gcaaattcgc cgcctgcgta
     8041 tggaaggcaa gcgcgtggcg ctggtaccta ccatgggcaa cctgcatgat ggccacatga
     8101 agctggtcga cgaagccaaa gcccgcgccg atgtggtcgt cgtcagtatt ttcgttaacc
     8161 cgatgcagtt cgaccgcccg gaggacctgg ctcggtaccc acgcaccttg caggaagact
     8221 gcgaaaagct gaacaagcgc aaagtggatc tggttttcgc gccctcggta aaagagatct
     8281 acccgaacgg tactgaaacc catacttacg ttgacgttcc agggctttcg accatgctgg
     8341 aaggcgccag ccggccgggg cattttcgcg gcgtgtcgac tatcgtcagc aagctgttta
     8401 acctggtcca gccggacatc gcctgcttcg gtgaaaagga ctttcagcaa ctggcgctga
     8461 tccgcaaaat ggttgctgat atgggctttg atattgagat tgtcggtgtg ccaattatgc
     8521 gcgccaaaga cggtctggca ctgagttccc gtaacggtta tctgacggca gaacaacgca
     8581 aaattgcgcc cggtctgtac aaagttttaa gttcgattgc cgacaaattg caggctggcg
     8641 aacgggatct cgatgaaatt attgctattg ccgggcaaga actgaatgaa aaaggcttcc
     8701 gcgccgatga tattcagatt cgcgatgccg acacattgct ggaagtctct gaaaccagca
     8761 aacgggcagt aattctggta gccgcctggc ttggcgatgc tcgcctgatc gacaacaaaa
     8821 tggtcgagct ggcgtaatac ttatactcta aataattcaa gttgcaagtt gcaggaaggc
     8881 ggcaagcgag tgacaaattc gtcaggaacg aatttgccca gccgcaggct ggccttcggt
     8941 gagagacatg gatgtctctc attaatcccc aggagcttac ataagtaagt gactggggtt
     9001 agcgaacgca gacgcagcac atgcaatttg aagtatgacg agtataactg gcgctacggc
     9061 tgatggcgcc aattattaat ttgccccacg tcatggcgat ggcgtctgta agcaattatt
     9121 cataacgcag acaacttagt taatcagcct atcaatctca gcaagagata aatttgtcat
     9181 ctctgcaacg tcttcccgag acatcccttt actcagaaaa cgctgggcga attcttgcct
     9241 ctcctcctgt cttccctgct gaatcccctt ctcaatccct ttctcttcaa accactgtgc
     9301 cagcgtcatc atagacttcc ctcccgtttc cctgtctctc agcacaccat aaaacaaatc
     9361 cgcttgttca gtatgaccgc gttgcagcat atagttttgc atggcaacta actgactttc
     9421 gctagtgtac ccttcgtcaa tcagcgtgac cagttgctcc agcaataaca ttaagtcgcg
     9481 ctggcgaata tgtttttgca gtagttcgag aatcgcaatc cgccgatgtt gcatgatttc
     9541 gtcatccggt gtgatggtga tatccaccag cgggaaagga ctgttataga cgcgtcgcgc
     9601 cagctccggc gagtaaaaca tatcaaacca gcacattgag agcggataag gtgtggcctc
     9661 gccctgataa aagagaatcg gcactaccag cggtagtttg tcgtgatccg cctccagatg
     9721 ccgatgcatg gcggctatag aataacgcat catgcgaaag gccattttct tatccggctt
     9781 gctttggtgt tcaatcacaa catgcagata accgggattg ccctgcattt gcacggaata
     9841 gagcacgtcc gtgctgtgtc ctttaaggct ctcttcaatg aaactccccg actctaaatg
     9901 aagcgtgttg aggtcacaaa gttcgcgtaa ttccactggc aaatgtatct ccagaaagtc
     9961 gcgagccgtc tccgcatgca ttaaaaattg tttaaatacc gcgtcatgcg gtgtggtact
    10021 cggtgcatcc atcttccctg tcttccgtgt tgtcttgcgt gaaggcagta tccagtcaag
    10081 gcgtgctatc gtcagccacc tctttattgt ttaccgagca gcgttcagag aaatatcaat
    10141 tcgttacagg cgatacatgg cacgcttcgg cgcggtacgc aaactgctga tgcaaatgcc
    10201 gtcagatgag tagacactaa acaaaaatcg ggcaatactg cgtgagaatt tcctcaaagc
    10261 aggcaccgcc tgcttcgtta acgacagggt agaaaggtag aagttatgat tcgcacgatg
    10321 ctgcagggca aactccaccg cgtgaaagtg actcatgcgg acctgcacta tgaaggttct
    10381 tgcgccattg accaggattt tcttgacgca gccggtattc tcgaaaacga agccattgat
    10441 atctggaatg tcaccaacgg caagcgtttc tccacttatg ccatcgcggc agaacgcggt
    10501 tcgagaatta tttctgttaa cggtgcggcg gcccactgcg ccagtgtcgg cgatattgtc
    10561 atcatcgcca gcttcgttac catgccagat gaagaagctc gcacctggcg acccaacgtc
    10621 gcctattttg aaggcgacaa tgaaatgaaa cgtaccgcga aagcgattcc ggtacaggtt
    10681 gcttgattgt tatccctgcg gctggttact caccagccgc gacatcgtct ccagcgaatc
    10741 cgttcttaag atataaagtc gttttagtaa caacggatta tcccccggtt ttactttgcc
    10801 tttcatggtt gtcaccgcca ggtgaaatcc ggcatcgttt gctgccttca cggctttgtc
    10861 attaaagccg ccaaacggat acgaaagata ccagacatgc ggattaaatt gcgccagagc
    10921 gcgacgtgaa cgtgcaaaat caaacagaat attgtgctca ctacggctca gtaatatggg
    10981 tcggcgataa ccatctaccc gatgcaaaaa atgggtatgt gactggaaat caaatacatc
    11041 gcgaatttcg ttaagctcag aaacgctcat aaattgcagc gattttggtt cccacttctg
    11101 cgggtgacgt ttgatgcgcg aggtaacaat aaacgccgtc gccttcatgc catattgttt
    11161 caacacagga tacgcatagc ggctcactga cttgaggcca tcatcaaagg taatcaccac
    11221 cgctcgcgca ggaagattga tcttattctt cacgtaacct tccagctgca ccatgctcag
    11281 tgtcgcgtat cccctgtcgc gcagccaggc catctggtta ttgaaagcgc gtaccgaggt
    11341 ggtcgtcgaa gtatggcgaa aacgggtgtt ttcttcgtcg cgcagaatat ggtgataggt
    11401 tagcaccgga aggccattat cgggttgggc atccagcgcg ctgatatagg ccagtcgatc
    11461 gccaatacgg atctgatacc aggtttgatt taacctgtct ttcagtttat gcaaaatcgg
    11521 gtagcgcaaa ttgtccgcca gtaccccaaa tggcgcactg cccacactcg gcgcgttata
    11581 gaccggcgta tctttccagg taactaagtt ctgattactc agcggcttgt tgaggtcacc
    11641 caaaccgtct tcaacttttt gtcgcccctg aaccggctca agatgacctt tatcgataaa
    11701 ccctttatca aagccaaaat taaatgcgta ataacttgcg gcagtgggct ccacggcaat
    11761 gatttgtccg gcccgaatat tccccacggt caccatcttg tcaccaattt gcgcccagac
    11821 cgcagcattt tcgatggttt gcatataacg ggcaggtaac gcggcactga cacttgcggt
    11881 aaacagcatc agcaggagaa taacagcttg tttgtacatg aaaaaatcac cgagggtgaa
    11941 acaacgcttt tattgtagca aaagcgccat cagaatcgcg ggttaatact catacaaatt
    12001 atgtttgagg acgaaaggcg gatttttttg ttgctggtgc caaatactac tcacctccgg
    12061 cgcacccagt tcgacaatac gctcgcgaaa ctctgaactg gtcatggttt cacggcaagc
    12121 catcatctgt tcaattaacg gtaacgtgac accagaaatc acttcgcaac gggagtgttt
    12181 gtggcttaat aatgaagcca cgcggtacgg tggcgctccg gctatatcgg ttaagaagat
    12241 gacaccctca ccagagtccg cttcatgcag agcatcgcac atcatgcggc tgagcatatt
    12301 agaacttaat ccgcgccaga aattcacggc ccggcactga agaagcgccc catgtttttt
    12361 ctccagcaca tccagtatct cttgcgcccg gtcatcgtga caggtaatta cccaacctaa
    12421 catcgccttc ctccttagcc gaaaatcagt ctaccctgac cattgttgat aaagggtgat
    12481 agccgtcaaa actcctctcc cccaaatcca gaggagagga aatagcctta gctacgcaaa
    12541 ccacgtccac gttggatcag cgaccaacag atcaaataaa acgccacaat aaagaccacc
    12601 agtacgccaa aggtagtgac cagcggaaca tcattgatac cgaggaagcc gtagcggaaa
    12661 ccactgatca tataaacgat tgggttcagg tgcgacagcc cttgccagaa cggcggcaac
    12721 aaagtcagtg agtaaaagac cccacccaaa tacgtgagtg gcgttaacac aaaggttggc
    12781 accaggctga tgtcatcgaa cgttttggca aacacaccgt tcagcaaacc cgcaagggag
    12841 aacaacaccg ccgtgagcac cagggttaag gcaacgaata cccacgaatg cacctgaaac
    12901 ggcacaaaaa acagtgaaat tgccgtcacc agaatgccaa caaacagacc acgcgccacg
    12961 ccaccgccga catatccggc aataatgacg tgagtcggaa ccggcgctac cagcagctct
    13021 tcaatattac gctggaactt ggcaccaaaa aatgatgacg cgacgttggc gtaggcgttg
    13081 gtgatcaccg acatcatgat cagccccggt acgatgaact gcatgtagct gaagccatgc
    13141 atatcgccaa tacgcgaacc aatcaggtta ccgaaaataa taaagtaaag ggtcatggtg
    13201 atgactggcg gcaccagcgt ctgcacccag atacgcataa agcgatggat ctctttcgcc
    13261 cagatgcttt ttagcgccac ccagtaaaga tgcatcatgc gcgatctcct tgtttttcat
    13321 taaccagtga aacaaacagc tcttccagac ggttagcttt gttacgcata cttaatacct
    13381 gaatgccctg ctcacttaac tgcgtaaata cgctgttgat cccctgctca cgcagcactt
    13441 caacttccag cgtcgcggta tcgaccagtc gatactgata gccatcgagc ttcggtaacg
    13501 gacttttcgg tgcgagatcg agaataaagg tttccgattt cagcttcgcc agcagcgcct
    13561 tcatcgaggt attttccacc agctcaccgt gttgaataat gccgatattg cggcacagca
    13621 tttctgcttc ttccaggtag tgtgtggtga gaatgatggt ggtgcctttg tcgtttaaat
    13681 ccttcaaaaa gccccacatt gagcggcgaa gttcaatatc cacgcctgca gtcggctcgt
    13741 cgagaatcag tagtttaggt tcatgcatta acgcacgggc aatcattaaa cggcgcttca
    13801 tcccgccaga taacatgcgc gcacgttcgt tgcgttttcc ccataaatcg agctgcttaa
    13861 gatatttttc gctgcggatg tacgcctctt tgcgctccac accgtagtac cctgcctgat
    13921 tcaccacaat ttgctgcacg gtttcaaacg ggttgaagtt aaattcctgc ggcaccagtc
    13981 ccaactgacg tttagcgttc acgacatctt tctcgagatc gtaaccaaat acgctgaccc
    14041 gcccggaggt tttatttacc agagagctga taataccgat agtggtcgat ttcccggccc
    14101 cgttcggccc gagaagcgca taaaaatcac ccgcttcgac ctgcaaatct attccacgaa
    14161 gcgcctgaac gccgcctgga tagttttttt taagctgttg aagttccagt gcaatggtca
    14221 taaattttta cttaccttac gttcttacac tttatctgtg gtttaaatcg tcccggagtt
    14281 gccctatatt agccaaacgt aattatttgg ttacaggtcg ttaacctcca tgaaagacat
    14341 agatacactc atcagcaaca atgcactatg gtcaaaaatg ctggtggaag aggatcccgg
    14401 gttttttgag aaactggcac aagcgcaaaa accgcgcttt ctatggattg gatgttccga
    14461 cagtcgcgtt cctgcagaac gtttaaccgg tcttgagccg ggcgaactct ttgttcaccg
    14521 taatgttgct aacctggtca ttcacaccga cctgaactgc ctttccgtgg ttcagtatgc
    14581 agtggatgta ctcgaagttg aacacattat tatctgtggc cactacggtt gcggcggcgt
    14641 acaagccgca gttgaaaacc cggaactggg gcttatcaac aactggctgc tgcatatccg
    14701 cgatatctgg ttcaaacata gctcattgct cgacgaaatg ccgcaagagc gccgtctgga
    14761 taccttgtgt gaactgaacg tcatggaaca ggtgtataac ctgggccact ccaccattat
    14821 gcaatcagcg tggaaacgcg ggcagaaagt taccattcac ggctgggctt atggcattca
    14881 cgacggcttg ctgcgtgatc tggatgttac tgccaccaac cgcgaaaccc ttgagcaacg
    14941 ttaccgtcac gggatttcca acctcaagct gaaacacgcc aaccacaaat aaaaatgcca
    15001 tgccggatgc aacacatccg gcaacttcac acttactcgt ccagcagaat cactttgccg
    15061 atatacggca gatgacggta acgctgtgcg taatcaatgc cgtaacccac cacaaactca
    15121 tccgggatcg agaaaccgat aaattctacc gggacgttca cttcacgacg ggacggttta
    15181 tccagcagcg tacaaatcgc caacgacttc ggttcgcgca ggcttaagat ctcacgcact
    15241 ttcgacagtg tattccccga gtcgatgata tcttcaacaa tcagcacatc cttgccacgg
    15301 atatcttcat ccagatcttt gaggattttc acatcacggg tggtggacat gccgctaccg
    15361 tagctggagg cggtcataaa gtcgacttca tgagatacct gaacttcacg gcacaggtcc
    15421 gccataaaca taaatgagcc acgcagcaga cccaccagca ccatatcgct gccgctgtct
    15481 ttgtaacgct cagtaatctg acgacccagt tcggcgatac gcgctttaat ctccgcttcg
    15541 gggatcatta cttctacagt atgtttcata tctctaacca tatgatttaa aaacaaatca
    15601 cttcgcacgg gcaggttttg gcaccgcaac aaagcgcaag acacgcagta taccagcaaa
    15661 acgattcatt cgcggaatct gttaataaag ctcaaattgt gatgacgatc acacatgtta
    15721 aaacctataa ttaattgcta ctaaaatatt aatgaattga aatggtgtct ctttatggca
    15781 attaacaata caggctcgcg acgattactc gtcacgctaa cagccctttt tgcagcgctt
    15841 tgcgggctgt atctactcat tggcggaggc tggctggtcg cgattggcgg ctcctggtac
    15901 taccctatcg ctggccttgt gatgctcggc gtcgcctgga tgctgtggcg cagtaaacgt
    15961 gccgcgcttt ggctatacgc cgccctgctg ctcggcacca tgatttgggg cgtctgggaa
    16021 gttggtttcg acttctgggc gctgactccg cgcagcgata ttctggtctt cttcggcatc
    16081 tggctgatcc tgccgtttgt ctggcgtcgc ctggtcattc ctgccagcgg cgcagttgcc
    16141 gcactggtgg tggcactgct gattagcggt agtatcctga cctgggccgg atttaacgat
    16201 ccgcaggaaa tcaacggcac cttaagcgcc gatgccacac ctgcggaagc tatctcaccc
    16261 gttgccgatc aggactggcc tgcctatggt cgcaatcagg aaggtcaacg cttttcacct
    16321 ctgaaacaaa ttaacgccga taacgtccat aatctgaaag aagcctgggt gttccgtact
    16381 ggcgatgtga agcagccgaa cgatccgggc gaaatcacca atgaagtgac gccgattaaa
    16441 gtgggcgaca ccctttacct gtgtaccgct caccagcgcc tgtttgcgct cgatgccgcc
    16501 agcggcaaag agaaatggca ttacgatcct gagctgaaaa ctaacgagtc tttccagcac
    16561 gtaacctgcc gtggcgtctc ttatcatgaa gccaaagcag aaacggcttc gccggaagtg
    16621 atggcggatt gcccgcgtcg tatcattctt ccggtcaatg atggtcgcct gattgcgatt
    16681 aacgctgaaa acggcaagct gtgcgaaacc ttcgccaata aaggcgtgct caatctgcaa
    16741 agcaatatgc cagacaccaa accgggtctg tatgaaccga cttcgccacc gattatcacc
    16801 gataaaacca tcgtgatggc aggttcagtt accgataact tctcaacccg cgaaacgtct
    16861 ggcgtgatcc gtggttttga tgtcaacacc ggggagctgc tgtgggcttt tgatccgggc
    16921 gcgaaagatc cgaacgcaat cccgtctgac gaacacacct ttacctttaa ctcgccaaac
    16981 tcgtgggcac cagcggccta tgacgcgaag ctggatctgg tgtatctgcc gatgggcgtg
    17041 accacgccgg atatctgggg cggtaaccgc acaccggaac aggaacgtta tgccagctcg
    17101 attctggcgc tgaatgccac aaccgggaaa ttggcgtgga gctaccagac cgttcaccac
    17161 gacctgtggg atatggatct tccggcacag ccgacgctgg cggacatcac cgttaatggt
    17221 cagaaagtgc cagttattta cgctccggcg aaaaccggca acatttttgt gctcgatcgt
    17281 cgtaatggcg aactggtggt tccggcaccg gaaaaaccgg ttccccaagg tgctgccaaa
    17341 ggcgattacg ttacccctac tcaaccattc tcggaactga gcttccgtcc gacgaaagat
    17401 ttgagcggtg cggatatgtg gggagccacc atgtttgacc aactggtgtg ccgcgtgatg
    17461 ttccaccaga tgcgctatga aggcattttc acgccgccat ctgaacaggg cacgctggtc
    17521 ttcccgggta acctgggaat gtttgaatgg ggcgggattt ccgttgatcc aaatcgtgaa
    17581 gtggcaattg ccaacccaat ggcactgccg tttgtttcga aactgatccc acgcggtccg
    17641 ggtaacccga tggagcagcc gaaagatgcc aaaggcacgg gtacggaatc cggcattcag
    17701 ccacagtacg gtgtaccgta tggtgtcacg ctcaacccgt tcctctcacc atttggtctg
    17761 ccatgtaaac agccagcatg gggttatatc tcggcgctgg atctgaaaac taatgaagtg
    17821 gtgtggaaga aacgtattgg tacgccgcag gacagtatgc cgttcccgat gcctgtaccg
    17881 gtgccgttca atatgggtat gccgatgctg ggcgggccaa tctccacggc gggtaacgtg
    17941 ctgtttatcg ccgctacggc agataactac ctgcgcgctt acaacatgag caacggtgaa
    18001 aaactgtggc agggtcgttt accagcgggt ggtcaggcga cgccaatgac ctatgaagtg
    18061 aatggtaagc agtatgtggt gatctccgca ggcggtcacg gttcatttgg tacgaagatg
    18121 ggtgactata ttgtggctta tgcgctgccg gatgatgtga agtaagactt gctcagattg
    18181 ctgacaacgt gcgcgttgtt catgccggat gcggcgtgaa cgccttatcc agcctacaaa
    18241 attgtgcaaa atcaatggat tgcacaggta acgtaggcct gataagcgta gcgcatcagg
    18301 cagatttgcg tttgtcatca gtttaatgcc cggagagatc cgggcatatt cccgaatacg
    18361 gtctttttat accgtaaacc ctaacatcat ccccgtatct tcatgctcca gcagatggca
    18421 gtgcgccata taagcacgtt ctttcggtgc gtcgtgatta aacttcacca gcacttcact
    18481 gacattgcct tctaccttaa cggtatcttt ccagcccgcg cgatgagccg ctggcggttt
    18541 gccattttct gacaagatac ggaactgcgt gccgtgaata tggaacggat gcagcatcat
    18601 gtcgcccacg ccagagataa cccaacgttc gtactgccct ttcgccgccg caaacatcgg
    18661 cttattcata tcaaacgcct gaccgttgat tttattggca tggtggaaat cgaacttccc
    18721 gccgtggttc atatgattca tattgccgtg ccccatatgg cccatcatct ggctgtgatc
    18781 cattccggcc atcgcctgat cgccatattt ctccattagc atctgcatcc ccatcatatc
    18841 gagcatcggg tccatagaaa gttgcagctt gcgtaccgtc agcccttcca gcgaaggtaa
    18901 cgcaggcagg ctacttaatg tgtctggcaa agcaccggaa gcactaatag caatcggctg
    18961 aatccgcatt accggatgag gcttatcaaa cggcgcaatc gccatcccca tctggctgac
    19021 cggcagcgtc accaggtcaa agggtttgtt gtcgttaacc tccaccagca cttcaaaacg
    19081 ctcgcccatc agcaccggca gctcgttcac cttcaccggt tcaggtagca gaccaccgtc
    19141 gctggcaatc acataaagcg ggcgattgtc gctggtggcg aaattgagcg agcgggcatt
    19201 acagccattg agcaaacgca ggcgcagcca accacgcggg gcagcgtgtt gcgggtagat
    19261 tgcaccgttg gtcagcaacg tatcgccaaa ccagcccacg gcggcggtca tcacatccag
    19321 ttgataatca atctgcccgt cggcgctaaa tttcttatcc tgaacgatca ccggaacatc
    19381 atcgataccc cactgttttg gcagcattaa tttcaggatc tcgtcatctt caatcaccac
    19441 cagaccagcc agccccatcg ccacctgtcg cccggtcttg ccatgttgat gtggatggaa
    19501 ccagcaggta gcggcaggtt gatcaacgtt caacgtcacc gagcgcttgc cacctggcgg
    19561 aataattccc tgtgggccgc cgtcgacttc acccggtact tccagcccgt gccagtgcaa
    19621 cgtcgtctct tccgtcagtt ggttatagat atcaaccgtt accgctttgc cgcgctgtaa
    19681 tttcaccgcc ggccccagca gattgccgtt atagccccag gtagttgcag ttttctcgcc
    19741 aaaggtagac tgacctgcgc caatagttaa ctgaatgcga ttacgggcat cggtcgtgag
    19801 caaatcaggg atcggtaacg ttgggcgttc cgccgcaaag actgcacggc tccacagtgg
    19861 caaggctgaa gccacaccca gtgcgacgga atatttcaag aaatcacgac gttgcatagt
    19921 tatttcctta ttcttaagca ggcgaaacaa aatcaaacgt tgagcatagt ccttcccctt
    19981 acgggaaggt caagccgcag acacaattta atcgacaata atggtcgtcg cctcgctcgc
    20041 agtgtgctaa cgtttatctt ctttaagccc ctggtagaag caatgaagac gtttttcaga
    20101 acagtgttat tcggcagcct gatggccgtt tgcgcaaaca gttacgcgct cagtgagtct
    20161 gaagccgaag atatggccga tttaacggca gtttttgtct ttctgaagaa cgattgtggt
    20221 taccagaact tacctaacgg gcaaattcgt cgcgcactgg tctttttcgc ccagcaaaac
    20281 cagtgggatc tcagtaatta cgacaccttc gacatgaaag ctctcggtga agacagctac
    20341 cgcgatctca gcggcattgg cattcccgtc gctaaaaagt gcaaggccct ggctcgcgat
    20401 tccttaagcc tgcttgccta cgtcaaataa tccctgatac ctttttgtag aaatattgac
    20461 cgtgcatccg cggtcaatgt tagctattat gttgcgccct ttttttacgg gtgttaacaa
    20521 aggaggtatc aacccatggc cgaaaaaaaa cagtggcatg aaacgctaca cgaccagttt
    20581 gggcagtact ttgcggtaga taacgttctg tatcatgaaa agaccgatca ccaggatctg
    20641 atcatttttg agaacgctgc atttggtcgc gtaatggcgc tggatggcgt agtacaaacc
    20701 accgagcgcg acgagtttat ctatcatgag atgatgaccc atgttccgct actggcccac
    20761 ggtcacgcga aacatgtgct gattatcggc ggcggcgacg gtgccatgct gcgtgaagta
    20821 acccgacata aaaacgttga gtcaatcacg atggtggaaa tcgatgcggg tgtcgtgtcg
    20881 ttctgccgtc agtatctacc caaccataac gccggtagct acgacgatcc gcgctttaag
    20941 ctggtgatcg acgatggcgt caatttcgtt aatcaaacca gccagacctt tgatgtcatt
    21001 atctccgact gcaccgatcc tatcggtccc ggcgaaagcc ttttcacttc ggcattttat
    21061 gaaggctgca aacgttgcct gaatcctggc ggtatcttcg tcgcacaaaa cggcgtctgc
    21121 tttttacagc aggaagaagc catcgacagc catcgcaaac tcagccatta cttcagcgac
    21181 gttggctttt atcaggcggc gatcccgacc tattacggcg gtatcatgac ttttgcatgg
    21241 gcgacagata acgacgcctt acgccatctc tcaaccgaaa ttattcaggc gcgttttctc
    21301 gcctctggcc tgaaatgccg ttattacaat ccggcagtcc atacggcagc ttttgcctta
    21361 ccccagtatc tgcaagacgc actggcttca cagccgtcct aaggagaaga taagaaattg
    21421 aaaaaactga aactgcatgg ctttaataat ctgaccaaaa gtctgagttt ttgtatttac
    21481 gatatctgct acgccaaaac tgccgaagag cgcgacggtt atattgctta tatcgatgaa
    21541 ctctataatg ccaaccgtct gaccgaaatc ctgtcagaaa cctgttccat tatcggggct
    21601 aatattctta acatcgcccg ccaggattac gaaccacagg gtgccagcgt cactattctg
    21661 gtgagtgaag aaccggttga cccgaaactc atcgacaaaa cagaacaccc cggcccactg
    21721 ccagaaacgg tcgttgccca tctcgataaa agtcatattt gcgtacatac ctacccggaa
    21781 agtcatcctg aaggcggttt atgtaccttc cgcgccgata ttgaagtctc tacctgcggc
    21841 gtgatttctc cgctgaaggc gctgaattac ctgatccacc agcttgagtc cgatatcgta
    21901 accattgatt atcgcgtgcg cggttttacc cgcgacatta acggtatgaa gcactttatc
    21961 gaccatgaga ttaattcgat tcagaacttt atgtctgacg atatgaaggc gctgtatgac
    22021 atggtggatg taaacgtcta tcaggaaaat atcttccata ccaagatgtt gcttaaagag
    22081 ttcgacctta agcactacat gttccacacc aaaccggaag atttaaccga cagcgagcgc
    22141 caggaaatta ccgctgcgct gtggaaagaa atgcgcgaga tttattacgg gcgcaatatg
    22201 ccagctgttt aacggctctg gcggagctcc caggctccgc cagatttatt tacttctgct
    22261 gcacgaaatt gcggtaagcc gccacgacct gcagaaaatc ctcaacgccg catagcgaca
    22321 ggctttcttc gtcgtagtag ttcatcccct cttccatttc atcgccagcg aattccagtt
    22381 gattggcgcg aaccatcacc tcttcgccgt ccatccatag cgtatactca tgccctgccc
    22441 gctgccagga acgttcgcta cctttcagtg cgtgcgctgc ttgttccact tcatcaagca
    22501 aggccaggtt ttctttcacc tcttcattaa accagtgccc gaccacttca tgccccatgg
    22561 acatacgcac ctttaccact ccggtaatat cgcgcagaaa ttcgtaatcc atagtgtgtt
    22621 cctctgctcc cggcaatgca ccgaaatcgc attgcgccat tgatgtaatt atcgcagcct
    22681 taccggagaa aaacagcgga acggcgatgg tttagaaata aaaaaatgcc cgggaatctc
    22741 attcaccggg cattgtgtcg tttatgcgca gcgcgtgcgc tgacttttta aaccgcagtc
    22801 tggaaaatca ccccatcggc tttctcggtg tactgagaaa gctggttgaa gttcagataa
    22861 cggtaagtat caacggctgt tttatctacc tgcgccacgt aggtctggta ctcttccggc
    22921 gtcggcagtt tgccaatcag cgccgcaaca gcagccagtt ccgcagaagc caggaagaca
    22981 ttcgcgccag tacccagacg gttcgggaag ttacgggtag aggtggaaac caccgttgca
    23041 ccgtccgcca cacgcgcctg gttacccata cacagggaac agccagggat ctcgatacgc
    23101 gcaccactct taccgaagac gctgtagtag ccttcttcgg tcaactgtgc ggcgtccata
    23161 cgggttggcg gtgccaccca caggcgggtc ggcaactgac ctttgtgcgc atccagcagt
    23221 ttacctgcgg cacggaagtg accgatgtta gtcatgcaag aaccgataaa cacttcgtcg
    23281 atcttctcgc cctgtaccgc agacagcgga cgtgcgtcgt ccgggtcgtt cggagcacag
    23341 aggattggct ctttaatatc cgccagatcg atgtcgatca ctgccgcgta ttccgcatct
    23401 gcatcggctt ccagcagctc aggattcgcc agccattttt ccatgccctg aatacgacgt
    23461 tccagggtac gacgatcgcc gtaaccttcc gcgatcatcc acttcagcag gacgatgttg
    23521 gagttcaggt attcgatgat tggttctttg ttcagcttga tggtacaacc ggcagcagaa
    23581 cgctcggcgg acgcatcggt cagctcaaag gcctgctcaa ctttcagatc cggcagacct
    23641 tcaatttcta ggatgcggcc agagaagatg tttttcttgc ctttcttctc aacggtcagc
    23701 agaccttgtt tgatcgcata cagcggaatg gcgtgtacca gatcgcgcag ggtgatgccc
    23761 ggctgcattt tgcctttgaa gcgcaccaga acggattccg gcatatccag cggcattacg
    23821 cctgttgcgg ctgcaaatgc caccagacca gaacccgccg ggaaagagat accgatcggg
    23881 aaacgggtat gggagtcacc accggtaccg acggtatccg gcagcagcat acggttcagc
    23941 caggagtgga ttacgccgtc acccggacgc agcgacacac cgccacggtt cataatgaag
    24001 tccggcagcg tgtggtgcgt gttcacgtca actggcttcg gatacgccgc ggtgtggcag
    24061 aaagactgca tcaccaggtc agccgagaag cccaggcacg ccaggtcttt cagttcatca
    24121 cgggtcatcg ggcctgtggt gtcctgagag cctacagaag tcattttcgg ttcacagtac
    24181 gcgcccggac gaatgccttt cacgccacag gcacggccta ccattttctg cgccagcgag
    24241 aagccgcgat cgctctcagc gacatctttc gcctgacgga acacatcact gtgcggcaga
    24301 ccaagtgctt cacgcgcttt ggtggtcagg ccacgaccga taatcagcgg aatacggccg
    24361 ccagcacgca cttcatcaat cagcacgtcg gttttcagtt cgaaggtcgc cagcagttcg
    24421 ccggtttcgt ggttacgtac ttcacctttg tacgggtaaa cgtcaattac gtcgcccatg
    24481 ttcaggttag agacatccac ttcgattggc agcgcacccg catcttccat cgtgttaaag
    24541 aagatgggtg caattttacc gccgaggcac aaaccaccgc cgcgtttgtt cggcacatgt
    24601 ggaatatcat cgcccataaa ccacagaacg gagttcgtgg cggatttacg cgatgaaccc
    24661 gtacccacaa cgtcaccgac gtacgccagc gggaaacctt tctgttgcag agcttcaatc
    24721 tgtttgatcg ggccaacaac acctggctgg tctggctcaa taccttcacg ggcgtttttc
    24781 agcatcgcca gcgcgtgcaa tgggatatcc gggcgtgacc acgcatccgg tgccggagaa
    24841 aggtcatcgg tgttggtttc gccagtgact ttgaagacgg taacggtcag tttttcagcc
    24901 agcgccgggc gattcaggaa ccattcggca tcagcccagg actgcataac ctgcttcgca
    24961 tattcgttgc ctgctttcgc tttctcttct acgtcataga agttatcgaa catcagtagt
    25021 gtgtgagaaa gtgctttggc agcgatcggt gccagtttgg catcatccag cgcatcgatc
    25081 agcggatgaa tgttgtaacc accctgcata gtacccagca gttcgatggc tttttccgga
    25141 gtcagcagtg gggatttggc ttcgcctttg gcgacagcag ccaggaagcc tgctttaaca
    25201 taggcggctt catcgacgcc aggaggaaca cggttggtta acagatctaa caggaattct
    25261 tcttcgcccg cgggcgggtt tttcagcagc tctacaagcg cggccatttg gtttgcatcc
    25321 aggggtttgg gcgcaatccc ctcagcggca cgctcagcta cgtgcttacg gtattcttct
    25381 agcacgacgg ttctcctcgc tctcattgtc atagtgcggc aggcgattct cttcacgctc
    25441 ctgtgagaca gcagtttgta gggtaaatgc ccagtaccgc aacgggcaga atagcaggat
    25501 tttgacgacg tgttaatctg tttacaaaaa agcaacataa aaaagtggct gaatcgttaa
    25561 ggatggtcta gagattgttt ccctccgtaa aaacttcgtt aattcgcatg gtgataatca
    25621 cctttctcaa cgaatcccaa gattagtcaa aatttaaact accgcctctt tatactcgga
    25681 ttcacagcac ctgcgggtgg cagttcgccg accattgcga tttccttgag atccgaatta
    25741 tgaaaatgac tttgccgttt aaaccccatg tgctggcgct aatttgcagt gccgggcttt
    25801 gtgccgcctc tgccgggcta tatataaaaa gccgcacagt ggaagcgcct gtggaaacgc
    25861 aatcgacaca actggctgtg tctgacgctg ccgcagttac gcttcctgca acggtttccg
    25921 cacctcccgt aacacccgcc gtcgtcaaat ccgcattcag cactgcacaa atagatcaat
    25981 gggtcgcgcc cgtcgcgctg tatcccgacg ccctactttc gcaggtgctg atggcatcaa
    26041 cctatccgac aaacgttgct caagcagtgc aatggtcgca cgataatcca cttaaacaag
    26101 gcgatgctgc tattcaggcg gtatctgacc agccgtggga cgccagcgtt aaatcactgg
    26161 tggcctttcc acaattgatg gcattgatgg gcgaaaaccc gcaatgggtg caaaacctgg
    26221 gcgatgcttt tctggcccag ccgcaggacg tgatggactc ggtacaacga ttgcggcaac
    26281 tggcacaaca aaccggctcg ctgaagtcat caaccgaaca gaaagttatt accacaacga
    26341 agaaaactgt accggtaaca cagacagtca cggctcccgt cataccatcc aataccgttt
    26401 caactgccaa ccctgtcatt acagagcctg caacaaccgt catttccatt gagcccggca
    26461 atcctgatgt ggtctatatt cccaactaca acccaaccgt ggtttacggg aactgggcca
    26521 atactgcgta tccgccggtt tatctgccac caccagccgg agaaccgttt gttgacagct
    26581 ttgtacgcgg attcggctat agcatgggcg ttgctaccac gtacgcacta ttcagcagca
    26641 tcgactggga tgacgacgat catgaccatc atcatcatga cgatgataat tatcatcacc
    26701 acgatggcgg tcatcgtgac ggtaatggct ggcaacataa cggcgacaac atcaatatcg
    26761 acgtcaacaa tttcaaccgt atcaccggtg agcatcttac tgataagaat atggcatggc
    26821 ggcacaatcc aaactaccgt aatggtgtgc cctatcatga tcaggatatg gcaaagcggt
    26881 ttcatcaaac tgatgtcaac ggcggaatga gtgccacgca gctacctgct ccaacacgcg
    26941 acagccagcg tcaggcggca gcaagtcagt ttcagcaacg aacacacgcc gcccccgtca
    27001 ttacacgaga tacccaacgt caggcagcgg cacagcggtt taatgaagct gaacactatg
    27061 ggagctatga cgacttccgc gacttcagcc gtcgccaacc actgacccag caacaaaagg
    27121 acgccgctcg tcagcgttat cagtcagctt ctcctgagca gcgccaggca gttcacgaga
    27181 aaatgcagac taacccgcag aaccagcagc gaagagaggc agcgcgtgag cgcattcagc
    27241 ccgcctcgcc tgagcagcgc caggcagtcc gcgagaaaat gcagactaac ccacagatcc
    27301 agcagcgaag agacgcagcg cgtgagcgta ttcagtcagc ctcgcctgag cagcgccagg
    27361 tgtttaagga aaaagtacag cagcgcccac tgaaccaaca gcaacgtgat aacgcccgcc
    27421 agcgtgttca atcagcatca cctgaacaac gtcaggtttt tcgggagaaa gctcaggaga
    27481 gccgcccaca acgtctaaac gacagtaacc atactgccag gctgaataac gagcaacggt
    27541 cagcagtacg cgaacgtctc tctgagcgcg gagcaaggcg actggaaagg taaattacag
    27601 gcgtaaaaaa agcggcgtgg ttagccgctt ttttaattgc cggatgttcc ggcaaacgaa
    27661 aaattacttc ttcttcgctt tcgggttcgg caggtcagta atgctacctt cgaacacttc
    27721 tgccgccagg cccacagact cgtgcagagt cgggtgcgcg tggatggtca gtgcgatgtc
    27781 ttcagcatca caacccattt caattgccag gccgatttca cccagcagct cgccgccgtt
    27841 agtaccgaca atcgcaccac cgatcacacg gtgagattct ttgtcgaaaa tcagcttggt
    27901 cataccgtct gcgcagtcgg aagcgatagc acgaccagaa gcagcccacg ggaaggtggc
    27961 ggtttcatag ctgatgcctt tctctttcgc ttctttctca gtcagaccta cccatgcaac
    28021 ttctggttcg gtataggcga tggacgggat aactttcgga tcgaagtagt gtttcttacc
    28081 ggcgataact tcagcggcaa cgtgaccttc gtgaacacct ttgtgtgcca gcatcggctg
    28141 accgacgata tcgccgatag caaagatgtg cggtacgttg gtacgcagct gtttgtcaac
    28201 gcggatgaaa ccacggtcgt caacttccac gccagctttg cctgcgtcga ggtttttacc
    28261 gttcggcaca cgaccaatcg ctaccagcac ggcgtcgtaa cgctgcggtt cagctggtgc
    28321 ttttttgcct tccatcgtca cataaatacc gtcttctttc gcttcaacgg cggtaacttt
    28381 ggtttccagc atcaggttga atttcttgct gatacgcttg gtgaagactt taacgatgtc
    28441 tttgtcagct gccgggataa cctggtcgaa catttcaacc acgtcaatct gtgaacccag
    28501 cgcgtggtaa acggtgccca tttccagacc gatgataccg ccacccatta ccagcaggcg
    28561 ttctggtact tctttcagtt ccagcgcgtc agtagagtcc cagatacgcg gatcttcatg
    28621 cggaataaac ggcagttgaa tcgggcgaga acccgctgca atgatcgcgt tgtcgaagtt
    28681 gatcacggtt ttaccgttct caccttcaac ttccagggtg ttagccccgg taaatttacc
    28741 cagaccgttg accactttga ctttgcggcc tttcgccata ccagccagac caccggtcag
    28801 ctgattgatc actttctctt tccaggtacg aatcttgtcg atatcggttt tcggttcgcc
    28861 gaagacgata ccgtgttcag ccagcgcttt ggcttcttcg ataacttttg ctacgtgcag
    28921 cagtgcttta gaagggatac agccgacgtt caggcaaaca ccgccaaggg tgttgtaacg
    28981 ttctacgatt acggtttcca gacctaaatc agcgcaacgg aaggcagcgg agtaacctgc
    29041 ggggcctgcc ccaagtacca cgacctgagt tttgatttca gtactcatca tgacctctat
    29101 atatttatct ccggcgggtc tgacgttttt tttatgacgc ccattatcca ccgggtcgtt
    29161 ctatccgtcg gtattttaca aaattgttaa caatttttaa acaacaaacg gcaaccgatt
    29221 tgtctattcg ctaataacct caatacattc atgagattac cagaaaaaag ccggccgttg
    29281 ggccggctct tttacttaca tcaccagacg gcgaatgtca gacagcgtgt tgttaatgat
    29341 ggtaatgaaa cgggcaccat cagcaccgtc gatcacgcgg tggtcgaagg agagagaaat
    29401 cggcagcatc agacgcggca cgaactcttt accattccac accggctcca tcgcggactt
    29461 ggaaacgccg aggatagcca cttccggcgc gttcacaatc ggcgcgaagt gggtagtacc
    29521 caggccgccg atgctggaga tggtgaagca accgccctgc atttcgcccg cagtcagctt
    29581 accgtcacgc gctttcttag aaatagtcat cagctcgcga gacagttcga tgatgccttt
    29641 cttgttgacg tctttgaata ccggaacaac cagaccgttc ggggtatcca ccgccacacc
    29701 gatgttgatg tatttcttca gggtcagacg ctgaccgtct tccgacagcg aactgttgaa
    29761 gcgaggcatc tgctcaagcg ctgcagcaac ggctttcatg atgaagacaa ccggggtgat
    29821 cttcacatcc agcttacgtt tcgccgcttc ttcgttctgc tgtttacgga acgcttccag
    29881 ctcggtgata tcggttttgt cgaagtgagt aacatgcggg atcatcaccc agttacggct
    29941 caggttcgca ccagaaattt tctggatacg gcccagttcc acttcttcga tttcaccaaa
    30001 cttgctgaag tccaccttcg gccacggcag catgccaggg ataccaccgc cagtcgctgc
    30061 cggagctgct tctgcacgtt tgatagcttc tttcacgtaa gcctgaacgt cttcgcgcag
    30121 gatacgacct ttacggccag tgcccttcac tttcgcaagg ttaacgccaa actcgcgtgc
    30181 cagacggcgg atcagcgggg tcgcgtgaac gtaagcgtca ttttcagcaa attcagattt
    30241 gccttccgct ttcgcagccg gtgctgctgc cggggcttca gcttttgctg ccggagccgg
    30301 cgctgctgct tcctgtttcg caggagctgc cgcaggcgct gcgccttcaa cttcgaagat
    30361 cataatcagc gagccagttt tcactttatc gccaacgttg actttcagtt ccttcacgac
    30421 gcctgcaaac ggagccggaa cttccataga agctttatcg ccttctacgg taatcagtga
    30481 ctgttcagcg gcaactttgt cgcccacttt caccatcact tcggtcactt caacttcgtc
    30541 accgccgata tccggaacgt taacttcttt cacgccagcc gctggtgcag gggccgctgc
    30601 cggagccgct tcctgtttag ccgccggagc tgccgcgcct gcttcacccg ccacttcgaa
    30661 gaccataatc agcgagccgg tagacacttt gtcgccggtg ttcactttga tttctttcac
    30721 ggtgcctgca aacggagccg gaacttccat agaagcttta tcgccttcta cggtgatcag
    30781 cgactgctca gcttcaactt tgtcgccaac tttcaccagg atttcggtca cttcaacttc
    30841 gtcgctgccg atatccggaa cgttaacgtc ttttgccgcg gcagcctcgg ggactgctgc
    30901 cggagctgct tctttcttct cttctgcctg agcaggtgca gcgtctgctg caccgtcggc
    30961 ggaatcgaaa atcataatca gtgcgccggt ctgagtttta tcgccaacag agactttgat
    31021 ctctttaacg atacccgcct gcggagacgg aacttccata gaagctttgt cgccttctac
    31081 ggtgatcagc gactgttcgg cttcaacttt gtcgcccact ttgaccagga tctcggtgat
    31141 ttcaacttca tcagccccga tgtccggtac tttgatttcg atagccatta ttcttttacc
    31201 tcttacgcca gacgcgggtt aactttatct gcatcgatgt tgaatttggc gattgcgtca
    31261 gcaaccactt tcttatcgat ttcgccacgt ttagccagtt cgcccagcgc cgcaaccacg
    31321 acataagaag catcaacttc gaagtggtga cgcaggttct cacggctgtc ggaacgaccg
    31381 aagccatcag tacccagtac gcggtagtcg tcagccggta cgtaagtacg gacctgctca
    31441 gcgaacagtt tcatatagtc ggtagatgcc acagccggag cgtcgttcat cacctgagcg
    31501 atatacggaa cgcgcggagt ttccagcgga tgcagcatgt tccagcgttc acaatcctga
    31561 ccatcacgcg ccagttcggt gaaggaggtc acgctataaa cgtcagaacc tacgccgtaa
    31621 tctttcgcca ggatctctgc cgcttcacgg acgtgacgca ggatagaacc ggagcccagc
    31681 agctgaactt tacctttgct accttcaatg gtttcgagtt tgtagatacc tttacggata
    31741 ccttcctcag caccttccgg cattgccggc atgtggtagt tttcgttcag cgtggtgatg
    31801 tagtagtaaa cgttctcttg tttttcaccg tacatacgct ccagaccgtc atgcataatg
    31861 acagcaactt cgtaagcgta agccgggtcg taagagatac agttcggaat agtcagcgac
    31921 tgaatgtggc tgtgaccatc ttcgtgctgc agaccttcac cgttcagggt ggtacgaccg
    31981 gaagtaccgc cgatcaggaa gccacgcgct tgctggtcgc cagccgccca gcacagatcg
    32041 ccgatacgct ggaagccgaa catcgagtaa tagatgtaga acggaatcat cggcagattg
    32101 ttggtgctgt aagaggtcgc cgctgccagc caggaacaac ctgcgcccag ctcgttgatc
    32161 ccttcctgca gaatctgacc tttctcgtct tctttatagt aagcaacctg ctcgcggtcc
    32221 tgcggggtgt actgctgacc gttcgggctg taaataccaa tctgacggaa cagaccttcc
    32281 ataccgaaag tacgcgcttc gtcggcgatg atcggtacca gacgatcttt gatcgacttg
    32341 ttcttcagca tcacgttcag ggcacgaacg aaagcgatag tggtagagat ctctttgctc
    32401 tgctcttcca gcagcgcgcc gaagtcttgc aggcttggca gctcaagctt ctcggtgaag
    32461 ttcggctgac ggcttggcag ataaccgtgc agtttctgac gctgtgcgtg cagataggta
    32521 tgctcttcag aaccttccgg gaaggtgatg tacggcagtt tttcgatatc tgcatcagac
    32581 accggcacat tgaaacggtc gcggatgtga cgcacaccgt ccatgttcat tttcttaacc
    32641 tggtgcgcga tgtttttacc ttcagccgcg tcgcccatgc cgtaaccttt aatggtatga
    32701 gcaaggatta ccgtcgcttt gcctttggtt tcctgcgctt tcttgaatgc agcgtagatt
    32761 ttcttcggat cgtgaccacc acggttcagt gcccagatct gctcgtcagt ccagtctgca
    32821 accagtgctg cggtttcagg atatttaccg aagaagtgtt cacgaacgta cgcaccatct
    32881 ttcgatttga aggtctggta gtcgccgtca acggtttcgt tcatcagctg gatcagttta
    32941 ccgctggtat ctttacgcag cagttcatcc caacggctac cccacatcac tttgatcacg
    33001 ttccagccag caccttcgaa gataccttcc agttcgttga tgatcttgcc gttaccggtg
    33061 accgggccgt caagacgctg caggttacag ttgataacga agaccaggtt atccagtttt
    33121 tcacgggtag cgatggtgat cgcacctttg gattccggtt cgtccatttc accgtcgccg
    33181 aggaacgcgt aaacggtctg tttagaggta tctttcaggc cacggtgttc cagatatttc
    33241 aggaatttag cctggtaaat agcaccaatc ggacccagac ccatagatac ggtcgggaac
    33301 tgccagaatt ccggcatcaa tttcgggtgc ggataggaag agaggccatt gccgtgaact
    33361 tcctgacgga agttatccag ctgctcttga gtcagacgac cttccaggaa agcacgagcg
    33421 tacacgcccg gggagatgtg gccctggaag taaaccaggt cgccgccatc ctgctcgttg
    33481 cgtgcacgga agaagtggtt aaagcacaca tcataaatgg ttgcggaaga ctggaaggac
    33541 gccatgtggc cgcccagttc gaggtctttt ttcgacgcac gcagaaccgt catgatggcg
    33601 ttccagcgga tagctgaacg aatacggcgt tccagttcca gattacccgg atactccggt
    33661 tgttcttcaa cggggatggt gttgatgtag ttgctgatac cggtgcctgc ggctacgttg
    33721 acaccgcctt tgcgggcttc agcaagcagt tggtcgatca gatactgagc acgctcaaca
    33781 ccttcttcac ggatgaccga ttcgatcgcc tggagccagt cgcgagtttc gatcggatcc
    33841 acgtcatttg ggaaacgttc tgacatgggt tattccttat ctatctaata acgttgagtt
    33901 ttctggaacc tgtcccattg aactctcgcc ggaaagctca ataagacagg ttctacgttt
    33961 agttgccgcg cttttatatg cgcttgattt acaacatctt ctggataatt tttaccagaa
    34021 aaatcactaa ttctttcgtt gctccagacg acgcagagaa cgctcacggc ggctttcttc
    34081 acggcttctg tcgagcaaaa tttcttcgat aaaggccaga tggcgatgcg atgcttcgcg
    34141 cgcttcttcc ggcttaccgg ccataatcgc ttcaaatatg cgggtgcggt gactactcac
    34201 cagcggcagc atctcgcgac gcgaatagag caattcgaag ttctggcgga cattctgggc
    34261 caacatcggc tccatacacc ttagcagatg aagcagaacc acattgtggg ccgcttcggt
    34321 gacggcaatc tgatactgga gtacggcgtt tgattccgcg tccagatcgc cagactgctg
    34381 cgccagttct atggcgtggt ggagttcacg gatgcgttcc ttgtcttcat cggtactacg
    34441 cagcgcggcg taataagcgg cgataccttc cagggcgtgt cgtgtttcga gcaagtcata
    34501 ctgtgattca ggatggtcgg agagcagctc caccagcgga tcgctgaagc tttgccatag
    34561 gctgctctgg acaaaagtgc cgccaccctg gcgacgaagc aacaagccct tcgcttcgag
    34621 acgttgaatc gcctcacgca atgagggacg ggagacgtca aactgttttg ccagttcgcg
    34681 ttccggtggg agtttttcgc ccgggcggag agtgccttcg aggatcaaaa actccagttg
    34741 ctgctcaatc acatcggaga gttttggttg gcggattttg ctgtaggcca tgagttcctg
    34801 tcttaagcca cttgccgaag tcaattggtc ttaccaattt catgtctgtg acgctaaagt
    34861 aacaaagtat tcaccttatg tccatacagg ttttgattga aatcatgaaa ctgtgcacat
    34921 tttaacaact tgacatatat aacgtttcaa agttgtaact atgcacaa
//
LOCUS       DBFIFI010000028        34723 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.028, whole genome shotgun sequence.
ACCESSION   DBFIFI010000028 DBFIFI010000000
VERSION     DBFIFI010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34723
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.028"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(17..532)
                     /gene="sfmF"
                     /locus_tag="N7B39_001326"
     CDS             complement(17..532)
                     /gene="sfmF"
                     /locus_tag="N7B39_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HGU2421337.1"
                     /translation="MRRVFFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALLPFSLRLEGCPPGSVAILFTGTPASDANLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVRPGVA
                     KADAIFMINYN"
     gene            complement(543..1550)
                     /gene="fimH"
                     /locus_tag="N7B39_001327"
     CDS             complement(543..1550)
                     /gene="fimH"
                     /locus_tag="N7B39_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HGU2421338.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNVFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAVKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            complement(1563..4172)
                     /gene="sfmD"
                     /locus_tag="N7B39_001328"
     CDS             complement(1563..4172)
                     /gene="sfmD"
                     /locus_tag="N7B39_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HGU2421339.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTFVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSAHGYIPPEEWDEGIPALLLNYNFTGNRGNGNDSYFFSELSGINIGPWRLRNNGSW
                     NYFRGNGYHSEQWNNIGTWVQRAIIPLKSELVMGDGNTGSDIFDGVGFRGVRLYSSDN
                     MYPDSQQGFAPTVRGIARTAAQLTIRQNGFIIYQSYVSPGAFEITDLHPTSSNGDLDV
                     TIDERDGNQQNYTIPYSTVPILQREGRFKFDLTAGDFRSGNSQQSSPFFFQGTALGGL
                     PQEFTAYGGTQLSANYTAFLLGLGRNLGNWGAVSLDVTHARSQLADDSRHEGDSIRFL
                     YAKSMNTFGTNFQLMGYRYSTQGFYTLDDVAYRRMEGYEYDYDYDGEHRDEPIIVNYH
                     NLRFSRKDRLQLNISQSLNDFGSLYISGTHQKYWNTSDSDTWYQVGYTSSWVGISYSL
                     SFSWNESVGIPDNERIVGLNVSVPFNVLTKRRYTRENALDRAYASFNANRNSNGQNSW
                     LAGVGGTLLEGHNLSYHVSQGDTSNNGYTGSATANWQAAYGTLGVGYNYDRDQHDVNW
                     QLSGGVVGHENGITLSQPLGDTNVLIKAPGAGGVRIENQTGILTDWRGYAVMPYATVY
                     RYNRIALDTNTMGNSIDVEKNISSVVPTQGALVRANFDTRIGVRALITVTQGGKPVPF
                     GSLVRENSTGITSMVGDDGQVYLSGAPLSGELLVQWGDGANSRCIAHYVLPKQSLQQA
                     VTVISAVCTHPGS"
     gene            complement(4203..4895)
                     /gene="fimC"
                     /locus_tag="N7B39_001329"
     CDS             complement(4203..4895)
                     /gene="fimC"
                     /locus_tag="N7B39_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HGU2421340.1"
                     /translation="MMTKIKLLMLIIFYLIISASVHAAGGIALGATRIIYPADAKQTA
                     VWIRNSHTNERFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVNSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(5115..5657)
                     /gene="fimA"
                     /locus_tag="N7B39_001330"
     CDS             complement(5115..5657)
                     /gene="fimA"
                     /locus_tag="N7B39_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HGU2421341.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            6137..7003
                     /gene="folD"
                     /locus_tag="N7B39_001331"
     CDS             6137..7003
                     /gene="folD"
                     /locus_tag="N7B39_001331"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HGU2421342.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            7005..7217
                     /gene="ybcJ"
                     /locus_tag="N7B39_001332"
     CDS             7005..7217
                     /gene="ybcJ"
                     /locus_tag="N7B39_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HGU2421343.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            7325..7846
                     /gene="ybcI"
                     /locus_tag="N7B39_001333"
     CDS             7325..7846
                     /gene="ybcI"
                     /locus_tag="N7B39_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HGU2421344.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FNFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(7882..9267)
                     /gene="cysS"
                     /locus_tag="N7B39_001334"
     CDS             complement(7882..9267)
                     /gene="cysS"
                     /locus_tag="N7B39_001334"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HGU2421345.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKVEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            9441..9935
                     /gene="ppiB"
                     /locus_tag="N7B39_001335"
     CDS             9441..9935
                     /gene="ppiB"
                     /locus_tag="N7B39_001335"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HGU2421346.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            9938..10660
                     /gene="lpxH"
                     /locus_tag="N7B39_001336"
     CDS             9938..10660
                     /gene="lpxH"
                     /locus_tag="N7B39_001336"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HGU2421347.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRTKVHKPWLQTLFLALPLFVRKRIAVRMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            10778..11287
                     /gene="purE"
                     /locus_tag="N7B39_001337"
     CDS             10778..11287
                     /gene="purE"
                     /locus_tag="N7B39_001337"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HGU2421348.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            11284..12351
                     /gene="purK"
                     /locus_tag="N7B39_001338"
     CDS             11284..12351
                     /gene="purK"
                     /locus_tag="N7B39_001338"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HGU2421349.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAE
                     RSEWPAVFERLGELAIVKRRTGGYDGRGQWRLRADETEQLPAECYGECIVEQGINFSG
                     EVSLVGARCFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLSATLEA
                     LIPLLPPEYASGVMWAQSKFC"
     gene            complement(12490..13383)
                     /gene="ybcF"
                     /locus_tag="N7B39_001339"
     CDS             complement(12490..13383)
                     /gene="ybcF"
                     /locus_tag="N7B39_001339"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HGU2421350.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVRTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTDDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGMPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(13380..14195)
                     /gene="ylbF"
                     /locus_tag="N7B39_001340"
     CDS             complement(13380..14195)
                     /gene="ylbF"
                     /locus_tag="N7B39_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HGU2421351.1"
                     /translation="MTIIHPLLASSSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(14206..15465)
                     /gene="ylbE"
                     /locus_tag="N7B39_001341"
     CDS             complement(14206..15465)
                     /gene="ylbE"
                     /locus_tag="N7B39_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HGU2421352.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGASGMEAAR
                     AVSEEMAEIYLERNMQLQIPSWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(15475..17142)
                     /gene="fdrA"
                     /locus_tag="N7B39_001342"
     CDS             complement(15475..17142)
                     /gene="fdrA"
                     /locus_tag="N7B39_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HGU2421353.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSGSSQAL
                     TQVRRWDSACQKLPDANLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAGTPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIMPASSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDAHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPTLRNQLITDLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDNQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIHPLSPAAQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            17459..18508
                     /gene="allD"
                     /locus_tag="N7B39_001343"
     CDS             17459..18508
                     /gene="allD"
                     /locus_tag="N7B39_001343"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HGU2421354.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHVVINPNFFSSS
                     ELFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRQAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            18530..19765
                     /gene="allC"
                     /locus_tag="N7B39_001344"
     CDS             18530..19765
                     /gene="allC"
                     /locus_tag="N7B39_001344"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HGU2421355.1"
                     /translation="MITHFRQTIEEALPWLSSFGADPAGGMTRLLYSPEWLETQQQLK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVILSGSHIDTVVNGGNLDGQFGALAARL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPDAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIRIDIDLWMDEEPVPMNKDL
                     VATLTELCESEKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            19776..20561
                     /gene="allE"
                     /locus_tag="N7B39_001345"
     CDS             19776..20561
                     /gene="allE"
                     /locus_tag="N7B39_001345"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HGU2421356.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFS
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGYAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(20789..21934)
                     /gene="glxK"
                     /locus_tag="N7B39_001346"
     CDS             complement(20789..21934)
                     /gene="glxK"
                     /locus_tag="N7B39_001346"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HGU2421357.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDANYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGEKVNAFYGITGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LVANGGNLARVASIEMEECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLRAV
                     NLEQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(21956..23257)
                     /gene="ybbY"
                     /locus_tag="N7B39_001347"
     CDS             complement(21956..23257)
                     /gene="ybbY"
                     /locus_tag="N7B39_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HGU2421358.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTEVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            complement(23314..24675)
                     /gene="allB"
                     /locus_tag="N7B39_001348"
                     /pseudo
     CDS             complement(23314..24675)
                     /gene="allB"
                     /locus_tag="N7B39_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
     gene            complement(24712..26103)
                     /gene="ybbW"
                     /locus_tag="N7B39_001349"
     CDS             complement(24712..26103)
                     /gene="ybbW"
                     /locus_tag="N7B39_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HGU2421359.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGLGPIFDYIPSG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPLMEPLS
                     RVSWFVGVIVAFA"
     gene            complement(26272..27150)
                     /gene="glxR"
                     /locus_tag="N7B39_001350"
     CDS             complement(26272..27150)
                     /gene="glxR"
                     /locus_tag="N7B39_001350"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HGU2421360.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     NVDTARQVTEAADIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(27251..28027)
                     /gene="hyi"
                     /locus_tag="N7B39_001351"
     CDS             complement(27251..28027)
                     /gene="hyi"
                     /locus_tag="N7B39_001351"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HGU2421361.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKNI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYR"
     gene            complement(28040..29821)
                     /gene="gcl"
                     /locus_tag="N7B39_001352"
     CDS             complement(28040..29821)
                     /gene="gcl"
                     /locus_tag="N7B39_001352"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HGU2421362.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(29911..30726)
                     /gene="allR"
                     /locus_tag="N7B39_001353"
     CDS             complement(29911..30726)
                     /gene="allR"
                     /locus_tag="N7B39_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HGU2421363.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(30804..31286)
                     /gene="allA"
                     /locus_tag="N7B39_001354"
     CDS             complement(30804..31286)
                     /gene="allA"
                     /locus_tag="N7B39_001354"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HGU2421364.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            31516..32442
                     /gene="allS"
                     /locus_tag="N7B39_001355"
     CDS             31516..32442
                     /gene="allS"
                     /locus_tag="N7B39_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HGU2421365.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            32511..33605
                     /gene="mnmH"
                     /locus_tag="N7B39_001356"
     CDS             32511..33605
                     /gene="mnmH"
                     /locus_tag="N7B39_001356"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HGU2421366.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLHAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQNLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAARLDAALTTQLTTGSTDGHLAWLVPLLEEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAQ"
     gene            33721..34086
                     /gene="ylbG"
                     /locus_tag="N7B39_001357"
                     /pseudo
     CDS             33721..34086
                     /gene="ylbG"
                     /locus_tag="N7B39_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         8792 a         9223 c         8420 g         8288 t
ORIGIN      
        1 acgaattaat ataatattaa ttataattga tcataaatat cgcatccgct ttcgccacac
       61 ctggccgaac accgctggct aacgctcgat aattggcaaa aaagtttagt gtgacattgc
      121 catttgcatc tacttcctca gtcgggctcg cctcccccag tgcgagccgg gagcgatcgc
      181 tattacgtaa ttcgatggcg acggtttgtg ccattgcggg atcatccaga gccagcaggt
      241 tggcatcaga tgccggcgtt cccgtaaata aaatcgcaac tgaacccgga ggacatccct
      301 ccagccgcag gctaaaaggg aggagtgccg tggtatcgcc agcgttcagt aattgtgtcg
      361 taggccatct gcctaaatct accgtcttat caatatccgc tgtgtttacg gtacaggaga
      421 aatcaacaac gttaccgtgc aaattgatat taatcgttcc taaagggtca gctgcccatc
      481 cactggaact ccacagtaac ccgcagaaac agctaaagaa tactcttctc attctccgta
      541 cctcattgat aatcgacgcg taaatacccc agcgcgctaa acggcccttc ggtcggtttt
      601 tgaccggtaa tactgatagg ccaggcgcga agtgtgacat tggctgccgc agctgcatcc
      661 agacggaaag gaataacgct attgagatcg ttaggcgtga tcggcgtatc gttctgatcg
      721 gcgacaataa aacctaaatc ctgattgtcc gacaccatcg cctgaccaga aacggcactg
      781 gcttccagac gcattgttaa ataagcctgc gcagcaacat tcgtacattt gaccgcaatg
      841 ctcttggttt gcggcatgac accagcaggc cgattacccg gccctgccgc actaaataac
      901 gatgcgccga tatcaccaaa atcaaattca acaatctgcc cggcatttaa ttcgcagttt
      961 tgcggtactt caacccggcc accaaaacta atggtataaa caggtgtaac caacggatcg
     1021 taaggcgttg atgtcacata cacggtaaac atcacctgtc tggggatctc caccatgtta
     1081 ataaagggcc gaataacctt taaacgaaat atcaaacgag aatccatcac cccgaacgga
     1141 aggttttgcg aaacgttagg atctgttccc atacgaatat aattttgcgg gggaaattga
     1201 atatccatca cactatcaac cagactcatc gcacctaata gataatcatt taattgcata
     1261 tatttataat tatcgatagt ttcctgaaca ataaagttag tgacataact acggtatgta
     1321 taattcacca gcgtgccggg tggacaaatt gctgagacac ctacccagcc ggatttttcc
     1381 ggtaaaacaa caatctgccc tggctggtta ttggtgctat taaagacatt tgagagatca
     1441 tagtgttcat cttctgctac gccagtacta tttgcacaaa cagtagccca ggatatgttt
     1501 gccagacaca aacatgccat cgccaataat aatctgcaga ttatcttcat tgataatttc
     1561 ctttatgagc caggatgtgt gcaaactgcc gaaataacag tgacggcttg ctgtaagctt
     1621 tgcttcggca atacatagtg cgcaatgcag cgtgagttcg cgccgtctcc ccactgaacc
     1681 agtaattcac cagacaatgg cgcaccactt aaataaactt gcccgtcatc acccaccata
     1741 ctggttattc cggtactgtt ttcccgtacc agtgatccaa acggcaccgg ttttccgccc
     1801 tgggtaacgg taatgagcgc ccgcacgcct atgcgggtat caaaattggc acgaaccaac
     1861 gcgccttgcg tcggcacaac gctgctaata tttttttcaa catcgatgga attccccatc
     1921 gtattggtat caagcgcgat acggttatac cgataaaccg tggcatacgg catcaccgca
     1981 tagccgcgcc agtcggttaa aatgccagtt tgattttcaa tgcgtacacc gcctgcgcca
     2041 ggcgctttaa tcaaaacatt ggtatcccct aaaggctggc tcagcgttat gccattttca
     2101 tgtccgacca caccgccaga cagctgccag ttaacgtcat gttgatcgcg gtcgtagtta
     2161 tacccgaccc ccagcgtacc gtaagcggcc tgccagtttg ccgtggcgct gcccgtatac
     2221 ccattattcg aggtatcacc ctggcttacg tgataactca ggttgtggcc ttccagtaag
     2281 gtcccaccta cacctgccag ccagctattt tgcccgttgc tgttacggtt ggcgttaaag
     2341 gaggcataag cgcggtcgag cgcattttcc cgggtgtagc gacgtttggt cagaacattg
     2401 aaaggcactg aaacattaag tccgacaata cgttcgttat cggggatccc tacagattca
     2461 ttccacgaaa atgagagtga ataactgatg ccaacccagc tgctggtata ccccacctga
     2521 taccaggtat ctgaatccga agtattccag tatttttgat gggtaccaga gatataaagc
     2581 gagccaaagt cattaagtga ttgtgaaata tttaactgca aacggtcttt acggctaaag
     2641 cgtaaattgt ggtaattcac gattatcggt tcatcccgat gctcaccgtc ataatcgtaa
     2701 tcatattcgt acccctccat tcgacgatac gcaacatcat caagggtata aaaaccttgt
     2761 gtcgaatagc ggtaacccat taactgaaaa ttggtgccga aggtgttcat cgatttcgca
     2821 tagaggaagc gaatggaatc cccctcatga cgactgtcgt cggctaactg actgcgcgca
     2881 tgggtcacat ccagcgacac tgcgccccag ttcccgaggt tgcgccccag ccctaataaa
     2941 aaggcggtgt aattggcaga taattgcgtc ccgccgtagg cagtaaattc ctgtggtaaa
     3001 ccgccgagtg ccgtgccctg aaaaaagaaa ggcgatgatt gctgactatt accgctacga
     3061 aaatcgcccg ccgtcaggtc aaatttgaaa cgcccttcgc gttgtaaaat tggcactgtt
     3121 gaatacggaa tagtgtaatt ctgctgattg ccatcgcgtt cgtcgatggt gacgtccaga
     3181 tcgccatttg aagatgtcgg gtgcaaatct gtaatttcaa aagcgccggg ggaaacatag
     3241 ctttgataga taataaaacc attttgccga atcgttagct gggccgccgt acgggcaatc
     3301 ccacgtaccg ttggggcaaa cccttgctgg ctatcaggat acatattatc ggaagaataa
     3361 agccgtacac cacgaaatcc aacaccatcg aaaatatcac ttcctgtatt gccgtctccc
     3421 attaccagtt cacttttcag cggaataatg gcgcgctgta cccaggtgcc aatattattc
     3481 cactgttctg aatgatatcc atttccgcga aaatagttcc aggaaccatt gttgcgtaaa
     3541 cgccacgggc caatattaat cccgctgagc tcactaaaaa aatagctatc gttaccatta
     3601 cctctgttac cggtgaaatt ataattcagg agtaaagcag gaataccttc atcccactct
     3661 tcaggcggaa tgtaaccgtg agcgctactc aacaacgcta tctgaggaat agtgatgttc
     3721 aggcgcatcg ctgcaaaatc aaagttaatt gtggcatcag gtacagcttt cagtaaattg
     3781 atgcatttat tgttttgctg ctgggctaac tcgggaaatg cactgctatt aaggccaatt
     3841 cgttcaagga gtacctggtt aaaacagggc attaacccac ctgatttatc acctgtattt
     3901 tctgttgtcg attcaaatac gatatcctgt gaaccaatga actcatcatt acgccagaga
     3961 tccacccgat acacgcccgc aggttgatga ttacctctct caaagcgcga taaatcagca
     4021 acgaatgtgc cattctctaa taaaaatgcc gggttgaaat aactttccgc atagcttaaa
     4081 aaggagggta aaatggcaag agatgaataa caaattccgg ccagacacca ggtatacctc
     4141 tgcggaatat ccgtagtagt gggtattttc ataattagct ctgatattgt cagatgattc
     4201 ggttagttta agttcacttc tctgaccggg gttactgaac cataatcatt aacggtctga
     4261 aatttcagct ttccctgcac tcctgagggt aagggaattt gtgaattaac tctgggtgca
     4321 gccatagcat tccccaactt ttggctgccc acttgtaagt taaccagggt gacataaaaa
     4381 ggtgatggat tatgaacatt gatatagtta ccggatcgcg aaaacttgag tgtatccggc
     4441 gcttctgcgg gtaattcttg tagttgaatt ggacggagaa ataacttcat gcgcgataaa
     4501 atcgccagtt gcaaaacatt cctgccgttc aatgcatttt tatctaccga aggaatcgtc
     4561 ttaacattca tccagaacag agactcacga tctgctgcca gcggtggacc ggtgtaaata
     4621 atacgcaaag tattttcgct tttgggttca ctaacaaata gtggcggtgt aatgatgaat
     4681 gacttttctt ttacaccgct gctgttttca atccacgaat tgaccagaaa acgctcattg
     4741 gtatggctat ttctaatcca taccgcagtc tgtttagcat cagcgggata aataatacgc
     4801 gtggcaccta atgcgatccc tccggcagca tggacgctgg ccgaaatgat taaataaaat
     4861 ataatgagca tcaataactt tattttagtc atcatagtta atacgccttg tgtattcctg
     4921 acggcaaaaa taagaaatat aaagaaatga ttgctttggt ttcttacaac acatcattcc
     4981 cttttcattt aataaagcgg catgatacaa agtgaacatg atttcgattt acattgccac
     5041 cataccgctt cagacaaccg gtacattact ttgttataag acgtgatata taattcaaaa
     5101 caacgtggtt ttgattattc gtatctcata ataaaagtcg cgtcagcatt tgcttgccct
     5161 gctgatgcac ttgtaccggt gcctttataa cgtgcagaaa aatgaagaac gttggtcccg
     5221 gggatcaagt tctggttggt tgagaagcta ttcccatcag gtttgagaat tgcggatgta
     5281 ttatcaagta tttcaatacc gacacccgtt gctgttgttg tattggtact ggatgcaatg
     5341 gccaataaat tatcattgat tgcatcagcc tgaccagaaa atgcaacggc agcattagcg
     5401 gcaacaacag gatcgcagtc gttcaactga atggtgaatg gaattaatgc agaggtatta
     5461 ccaacagcat tgaaaatatc ggtacgatat tgaccgagtg tgacaacctg gtctgccgag
     5521 tcagtattca ctgaacaggc agcattcacc agttcacctt caaagtgaat tgtaccgcca
     5581 tctacaacgg cagcataact acccgtagcg gcgaatagtg cggcagccag cgcagacgaa
     5641 ataaatctta atttcatata tattccttca atctcattta tcgactccac atccgtatat
     5701 aaccgattac tttatttaag acactgatag tagtaaattc ctttttatcc tctaagaatg
     5761 tcttaattga aaatatgcac tctattctaa aaaatagaga gccccgttag atgaatactt
     5821 ccgcgcaaaa tatattcaac acaaatatag acctgaagcg gtaaattacc aggctgaaaa
     5881 ttctttttat attgtcaggt atttcttaaa ttatcttaat ccttaaacaa ggaaataaat
     5941 cagttccaga tttacaacgc catcatggac gaaaaatgaa gctttcaatc tcagcgacct
     6001 cagcgacagt gcgcctaacc ttcgcaagag gtcgcttcac gcgataaatc tgaaacgaaa
     6061 cctgacagcg cgccccgctt ctgacaaaat aggcgcatcc ccttcgacct acgtaacaga
     6121 tggaatcctc tctctgatgg cagcaaagat tattgacggt aaaacgattg cgcagcaggt
     6181 gcgctctgaa gttgctcaaa aagttcaggc gcgtattgca gccggactgc gggcaccagg
     6241 actggccgtt gtgctggtgg gtagtaaccc tgcatcgcaa atttatgtcg caagcaaacg
     6301 taaggcttgt gaagaagtcg ggttcgtctc ccgctcttat gacctcccgg aaaccaccag
     6361 cgaagcggag ctgctggagc ttatcgatgc gctgaatgcc gacaacacca tcgatggcat
     6421 tctggttcaa ctgccgttac cggcgggtat tgataacgtc aaagtgctgg aacgtataca
     6481 cccggacaaa gacgtagacg gtttccatcc ttataacgtc ggtcgtctgt gccagcgcgc
     6541 gccgcgtctg cgtccctgca ccccgcgcgg tattgtcacg ctgcttgagc gttacaacat
     6601 tgataccttc ggcctcaacg ccgtggtgat tggcgcatcg aatatcgttg gccgcccgat
     6661 gagcatggaa ctgttgctcg caggttgcac caccaccgtg actcaccgtt tcactaaaaa
     6721 tctgcgtcac cacgtagaaa atgccgatct gttgatcgtt gccgtcggca agccgggctt
     6781 tattcctggt gactggatca aagaaggcgc aattgtgatt gatgtcggca tcaaccgtct
     6841 ggaaaatggc aaagttgtgg gcgacgtcgt gtttgaagac gcggctaaac gcgcctcata
     6901 cattacgcct gttccgggcg gcgttggccc gatgacggtt gccacgctga ttgaaaacac
     6961 gctacaggcg tgcgttgaat atcatgatcc acagggtgag taacatggcg acattttctt
     7021 taggtaaaca tccgcacgtt gagctgtgcg acttgctgaa actggaaggc tggagcgaaa
     7081 gcggcgcgca ggcgaaaatc gcgattgccg aaggccaggt gaaagtcgac ggcgcggttg
     7141 aaacgcgcaa acgctgcaaa atcgtcgccg ggcagacagt gagttttgca ggtcacagcg
     7201 tacaggttgt tgcctgatcc ctttcccggc ctgtatcaac aggccggttc tcttttcaga
     7261 cttttgccct ctacgccccg ttcatccatg ctgatagcat cgacattttg ccgaagcgac
     7321 catcatgcca accgttatta cgcacgccgc cgttccccta tgcattggtt tagggttggg
     7381 ttcgaaagtc atccctccgc gcctgttatt tgccggaatc atcctggcga tgctgcccga
     7441 tgccgacgtc ttgtcgttta attttggcgt tgcttacggc aatgtttttg gtcatcgcgg
     7501 gtttacccat tcgctggtgt ttgcgtttgt tgtcccgctg ttatgtgtgc tgattggacg
     7561 tcgatggttc agggctgggc tgattcgctg ctggctgttt ttaaccgtct cgttgctatc
     7621 gcacagcttg ctggattcgg taaccactgg cggtaaaggg gttggctggc tgtggccgtg
     7681 gtcagatgaa cgctttttcg ctccctggca ggtgattaaa gtcgcgccgt ttgcattgtc
     7741 tcgttacacc acagcgtacg ggcatcaggt gattatttct gaattgatgt gggtgtggtt
     7801 gccggggatg ctactgatgg gaatgttgtg gtggcgcaga cgataaccgg atgcgaaaac
     7861 tcgcatccgg caatagcgca attacttacg acgccaggtg gtcccttgcg ggccatcttc
     7921 cagcacgatc cccatctcat taagacggtc acgcgccgca tctgccgccg cccagtcttt
     7981 cgctttacgg gcatccagac gctgttgaat taacgcttca atctcagcca cttcgctgtc
     8041 gtctgcctgc gcgccgcttt gcaggaacgc ttccggttct tgctccagca ggcccaatac
     8101 ggcggaaagt ttacgcaggt gagatgccat tgcattcgcc gctgccatat cttctacttt
     8161 cagacggttt acttcacgcg ccatatcaaa cagcacggaa taggcttccg gggtgttgaa
     8221 atcgtcgtcc atcgcttcaa taaagcgcgc ttcaaacgct tcgccaccgg caggcgcaac
     8281 ggttttatct gtgccgcgca gcgcagtgta gagacgctcc agcgccgcac gcgcctgctt
     8341 caggttctct tcgctatagt tcagctggct gcgatagtgg cccgacatca ggaagtaacg
     8401 cacggtttcc gcgtcgtagt atttcagcac atcgcgcacg gtaaagaagt tacccagcga
     8461 tttggacatc ttctcgcggt caaccatcac catccccgag tgcatccagt agttcacata
     8521 ctgaccatca tgggcacagg tggactgcgc gatttcgttt tcgtggtgcg ggaacatcag
     8581 gtctgaaccg ccgccgtgga tatcaaagtg gttacccagc tgcttgcagt tcattgccga
     8641 acattcaatg tgccagccag gacgacccgc gccccacgga gacggccagc tcggttcgcc
     8701 ctctttcgac atcttccaca gaacgaagtc cattgggttg cgtttgtcgt cgaccacgtc
     8761 aacgcgcgcg cctgcctgca gctggtcgag atcctgacgc gacagcacgc cataagttgg
     8821 atcggtcggg acgtcgaaca tcacgtcgcc gttgtccgcc acataagcgt gacctttggc
     8881 gatcagttgt tcagtgagtt caataatttc tgcgatatgg tgcgtcgcgc gcggctccat
     8941 atccgggcgc agaatgttca acgcatcaaa atctttgtgc atttcggcga tcatgcgatc
     9001 caccagcgcc acaaagcttt cgccattttc attggcgcgt ttgatgattt tgtcgtcgat
     9061 atcggtaatg ttgcgcacat acttcagctt atagccgagg aaacgcagat agcgcgcaac
     9121 cacgtcaaaa gcaacaaagg tacgcccgtg accgatatga cagagatcgt aaacggtgat
     9181 tccacacacg tacatgccga cttccccggc gtgaataggc ttaaattcct ctttttggcg
     9241 tgtcagagta ttgaagattt ttagcatcga agattccgtt tagacatgtg tgggtaattg
     9301 agttgcgtat aatacccata tttcccgcgc gaatcagcat acattgcgtg atgatccgat
     9361 cgtgcggtta tgctataaca ccaccctata tatgacccga actgggttga agcaccaatc
     9421 aaacggaaca ggatgcaaaa atggttactt tccacaccaa tcacggcgat attgtcatca
     9481 aaacttttga cgataaagca cctgaaacag ttaaaaactt cctggactac tgccgcgaag
     9541 gtttttacaa caacaccatt ttccaccgtg ttatcaacgg ctttatgatt cagggcggcg
     9601 gttttgaacc gggcatgaaa caaaaagcca ccaaagaacc gatcaaaaac gaagccaaca
     9661 acggcctgaa aaatacccgt ggtacgctgg caatggcccg tactcaggct ccgcactctg
     9721 caactgcaca gttcttcatc aacgtggttg ataacgactt cctgaacttc tctggcgaaa
     9781 gcctgcaagg ttggggctac tgcgtgtttg ctgaagtggt tgaaggcatg gacgtggtag
     9841 acaaaatcaa aggtgttgca accggtcgta gcggtatgca ccaggacgtg ccaaaagaag
     9901 acgttatcat tgaaagcgtg accgttagcg agtaatcgtg gcgacactct ttattgcaga
     9961 tcttcatctc tgcgtggaag aaccggcgat caccgccggt tttctgcgtt ttttagcggg
    10021 agaagcccgc aaggccgacg cgctgtatat tcttggcgat ctgtttgaag catggattgg
    10081 cgacgacgat cccaacccac tccatcgcca gatggcggca gcgatcaaag cggtgtccga
    10141 ttccggcgtt ccctgttatt tcattcatgg caaccgtgat tttctgctcg gcaaacgctt
    10201 tgcccgtgaa agtggcatga cgttattgcc ggaagaaaaa gtgctcgaac tttatggtcg
    10261 tcgggtgctg attatgcatg gcgacacgct gtgcaccgat gacgcgggtt atcaggcttt
    10321 tcgcaccaaa gtccacaaac cctggctgca aacgctattc ctcgctctgc cgttgtttgt
    10381 gcgcaaacgc attgccgtgc gaatgcgcgc gaacagtaaa gaagccaaca gcagtaaatc
    10441 gctggcgatc atggacgtta accaaaacgc ggtagtcagt gcgatggaaa aacatcaggt
    10501 gcaatggctg atccacgggc atacccatcg cccggcggtg catgaactta tcgccaatca
    10561 gcaacctgct tttcgcgtgg tactgggtgc ctggcatacg gaaggttcga tggtgaaagt
    10621 cacggcggat gacgttgagc tgattcattt tccgttttaa aaaacccgca actttgctga
    10681 tttcacagcc acgcaaccgt tttccttgct ctctttccgt gctattctct gtgccctcta
    10741 aagccgagag ttgtgcacca caggagtttt aagacgcatg tcttcccgca ataatccggc
    10801 gcgtgtcgcc atcgtgatgg ggtccaaaag cgactgggct accatgcagt tcgccgccga
    10861 aatcttcgaa atcctgaatg tcccgcacca cgttgaagtg gtttctgctc accgcacccc
    10921 cgataaacta ttcagcttcg ccgaaagtgc cgaagagaac ggttatcagg tgattattgc
    10981 gggcgcaggc ggcgcagcgc acctgccagg catgattgcc gccaaaacgc tggtgccagt
    11041 gctgggcgtg ccagtacaga gcgccgcact gagcggtgtc gatagcctct actccatcgt
    11101 acaaatgcca cgcggcattc cggtgggtac gctggcgatt ggtaaagctg gcgcggcaaa
    11161 cgcggcatta ctggcagcac aaattcttgc gactcatgat aaagaactgc accagcgtct
    11221 gaatgactgg cgcaaagcac aaactgatga agtgctggaa aacccggacc cgcgaggtgc
    11281 ggcatgaaac aggtttgcgt cctcggtaac gggcagttag gccgtatgct gcgtcaggca
    11341 ggcgaaccgt taggcattgc tgtctggccg gtcgggctgg acgctgaacc ggcggcggtg
    11401 ccttttcaac aaagcgtgat taccgctgag atcgaacgct ggccggaaac agcattaacc
    11461 cgcgagctgg cgcgccatcc ggcctttgtg aaccgcgatg tgttcccgat tattgctgac
    11521 cgtctgactc agaagcagct tttcgataag ctccacctgc cgactgcacc gtggcagtta
    11581 cttgccgaac gcagcgagtg gcctgcggtg tttgagcgtt taggtgagct ggcgattgtt
    11641 aagcgtcgca ctggtggcta tgacggacgc ggtcaatggc gtttacgcgc cgatgaaacc
    11701 gagcagttac cggctgaatg ctacggcgaa tgtattgtcg agcagggcat aaacttctct
    11761 ggtgaagtgt cgctggttgg cgcgcgctgc tttgatggca gcaccgtgtt ttatccgctg
    11821 acgcataacc tgcatcagga cggtattttg cgcaccagcg tcgcttttcc gcaggccaat
    11881 gcgcagcagc aagcgcaagc cgaagagatg ctgtcggcga ttatgcagga gctgggctat
    11941 gtgggcgtga tggcgatgga gtgttttgtc accccgcaag gtctgctgat caacgaactg
    12001 gcaccgcgtg tgcataacag cggtcactgg acacaaaacg gtgccagcat cagccagttt
    12061 gagctacatc tgcgggcgat taccgatctg ccgttaccgc aaccagtggt gaataatccg
    12121 tcggtgatga tcaatctgat tggtagcgat gtgaattatg actggctgaa actgccgctg
    12181 gtgcatctgc actggtacga caaagaagtc cgtccgggcc gtaaagtggg gcatctgaat
    12241 ttgaccgaca gcgacacatc gcgtctgtcc gcgacgctgg aagccttgat cccgctgctg
    12301 ccgccggaat atgccagcgg cgtgatgtgg gcgcagagta agttctgtta acctggtgct
    12361 catttgattg ccggatgcgg cgtgaacgcc ttatccggcc tacaccgatc cggtacccat
    12421 tgtaggcctg ataagacgcg tcaagcgtcg catcaggcat ggtgcgccga ttgccggatg
    12481 ctctcatcac tacagcgaaa tacaggtccc cgcttcgccc gccagcgtct cttcaattcg
    12541 cgataacgcc ccaatccacg cgggtttacc acggcttctg acataaccac tcaccgccgt
    12601 taccttcggc cccatcgaac catcggcttt ggcaaatggc gctaactcat ccggtgtggc
    12661 atggcgaatg gcacgttgct gcggcattcc ccagttttca tataccgcat cagcatcggt
    12721 gaggatcacc agtccatcgg cgttaatttg ctcggcgagc aacgcagcgg cgagatcttt
    12781 atcaatcact gcttcactcc ctgctccgtc atccgtcaca ggcacaccgc cgccgccact
    12841 gcaaatcacc acatgcccct ctttgagcaa caactcgatg gcttcgctgt cgagaatttt
    12901 acgcggttgc ggagacgcca ccacccggcg caaatattta ccatcacgtt tcatctgcca
    12961 gccgtaagcc gcttccagtg cctcttgttc ttctggctga taaaccggac caataaattt
    13021 ctctggctgc aaaaacgccg gatcatcagg cgaaacttca atgcgcgtcc gcaccgtcgt
    13081 gacaggcggc atctgcggct gtgcgctcaa actctgcgcc agcatatagc caatcatccc
    13141 ctgactttct gccaccagca catccagcgg atacggttct acctctttcc acgccagatt
    13201 ctgcaatgcc agcagcccca cctgcggccc gttgccgtga acaatcgcca gccgataaga
    13261 acgggccagg cgcgccagcg cgggtacagc actggcgata ttgcgatatt gattttctgc
    13321 cgtcagcgcc tcaccgcgct ggagtaaggc gttgccccca agagctacaa ccagtgtttt
    13381 catggttttc cttgtaataa ttgttggcca agccagaacc ccagcagtgt atctgcccct
    13441 gacgtatgcc ccagcgccag cagcgaatca atcgcaacag cggtacgttt cgggcaactc
    13501 agagcatgaa caaagtgcag gagtggcgag gcgaaatatc cttgcgcggc ataacgtaaa
    13561 taactgacgc tcaccgctgt ggtaacgagt tgcagattgt cggaacaggc aaaaaacgga
    13621 cgaccggagc gcgcatctaa agcaccataa taccaggccg ccagcagcat tccgctcagc
    13681 gtgtcatcat gacttggcgt taatccgggg cctttaccca gccagtgccg ccagtcggtc
    13741 ttaacgccat tgagcgcggc ctgaaaacag tgacgaaact ggcgtaactc agcaggcagc
    13801 ggatcgcttg ccgccaacgc cagtggcccg aaaagcccgg tttcctccgc gcgttgcatc
    13861 catgcagctg caagtggttg aggatgcgca ggcggcgtaa tacgcagcaa acaataacgc
    13921 tgtggctgtt taaccgtgaa acgcccgagg cgaatccctt gagccacaac ctgtggtcgt
    13981 tcattgccgc ataatccacc gcataacgca tcgaattgcg cacggcgaag cacccatcct
    14041 ccggggccga aaccactacc ctgacgatgc agcgttaaca gttcgccgct ttccgtcatc
    14101 aggttaatcg cccgccgcca cactcccgct aaccgccagg actggcgata attcggtgcg
    14161 ctactactgg caagcagagg atggatgatc gtcattacgc gttcctcaac caatccccat
    14221 gctttcagcc agtgcttcca gcgcctgttc aaagcacgcc agcggtgccc gcacggtgcc
    14281 tgcgccaatc tgcccgatcc ccgcctcttt atgggcgata ccggtattga tgagtggcgt
    14341 aatgccggtt tctaccacgc gacgaatgtc cagtcccagg cacgcgccct gaaaatccca
    14401 gcttgggatc tgcaactgca tattgcgttc aaggtaaatt tccgccatct cttcagatac
    14461 cgccctggcc gcttccatgc cactcgcacc gacaaagcgc gttacgccag gcgctgcgat
    14521 cattgccgca cctccgatac caaaggtttc ggtaatcgcg ctatcgccca tatccgggtt
    14581 cgcctgctcc tgcgagaagc cggtgaaaaa cagaccttgc ggagtgttta caggcgcagt
    14641 aaaccagcgt tcgcccagcc cgcttacccg aatcccgaac atattgccgt tgcgggtcat
    14701 tgccgtgacg atgctgcctg cgcggatcat cgcgccagca tccatcgccg ccttgcagta
    14761 agccatcgcg aggttgagga agaactgatc ggtcacgctg aggaaatcca tcacttccgc
    14821 gatgtgctgt ttatcatgat cgaggcgagc aatttgtggg gccagcgcac gcattaacag
    14881 tgctgaggaa gcaatattgc gttgatggaa ctcatcgccc atcgtaatgc cctgcgccat
    14941 catcgcagta agatcgatac cgcgctccat gcgccccagc gccgcgctta atactggcat
    15001 taacacatcg cgcatccagc gatggcgagt caggacatct tcgccgtaag cgccaaaacg
    15061 catcactttg ccgatacctt cgttgaggtt gcagtacgcc cggttgccgt cggtcacgtt
    15121 ctcgaccacc agcatcggca tactggcaga agtaataccg cccattggcc cgacggcatt
    15181 cacatggtga caaggaatga agttcacttc cccctgctcc agtattgcca gcgcctgcgc
    15241 ttcatctttc gcccaacctt cgaacagaca tgcgcccacg cacgcccctt tcatgggtcc
    15301 ggtcatctcc tgccagcgca ttggcggccc ggcgtgaagc agtgttttgc cctcgtttag
    15361 ttcgctgata agtgaagaag ccggttgcac atccagccag tgtggacgag cgcgacgaat
    15421 ttgttcgata accgcagcat tggcttgcgc cactgatgta aacatgagaa ccccttattg
    15481 caaacgttct aataaacgag ccagtttttt attgccgccg gcgactggcg accattggta
    15541 atgcacaacc ggtttgctgg cgctttgtag ctccagcgca aaactgcgta atccgatgtt
    15601 aatcacggcg acgttttcca gtaatgacgg tgtgtgttgc tgtgcggcag gcgagagcgg
    15661 atgaattaac gctgccgcca gcaaggtggc ttccggtagc gaactcacga ccgcaatccc
    15721 cgcatcttcc agcgtggcga tttgctgcga gcggcattgc gggtcacgtt cagtgcctgt
    15781 caccgtggca atggcataca gtggttgatt gtctgaacgc gcagcacagg ctttttgcca
    15841 ggcgcttacc agcgaggcgg caggatcggc ggtcgcaccg aagccaatca cgacatcaag
    15901 cagcagcaca cgcacttgcg gtttagcgcc gagatcggta attaactggt tgcgtaaggt
    15961 tgggtcgatc atcggatggg gacgcccgac ggtgtagaaa tcatcgccga ggtcaataat
    16021 ctggtggcca tcggcgtcca gcatcatgcc gtgatgatgg gcatcatcgg cttccacgcc
    16081 aaggtgtccg gcaagtaatc ccgccgcttc ggcagccagc gtaccgccgg tatacaaacc
    16141 gcaaataaat ccgctgctgg caggcattat cgcgttacgt cgcgccgtaa cgcgtgaaag
    16201 caggcaagcc aggcgtgcgg cctcatccag cgaggaggca aaccagacat tctcgtcgcg
    16261 ggccaccgcc ggggtataac ctaaaaacag cgccaccgtc ggtttgccgg ttgctttcat
    16321 ggcattaaca attttcagac gcaccgcttc ggcaggtggt tttgaaacaa atgccagcac
    16381 ttcgcttttc tcgtctgcac tgagcatttc cagcgccgtc agcgcactga tgccgcccac
    16441 ttcacggctg aggtcgcggc cgccaaggcc aatcgcgtga gtaattccct cccctgccag
    16501 cgcaatctgc gagcacagct cctgaatccc ggtaccggaa gcgccaatga cgccaatgtt
    16561 gccttccggc atcacgttag caaaagccag cggcgtgccg gcaatcatcg acgtaccgca
    16621 gtccggcccc atcaccagca agcctttttc ccgcgcgcgg gttttaagtt ggatttcatc
    16681 ttccagcgtg acgttatcgg agaacatcat cacgttgagg ttgcgatcca gcgcctgatt
    16741 cgccagctcc gccgcatact cgccagccac tgaaatcagc gccagattgg catcgggtaa
    16801 tttctgacag gcactgtccc agcgacgcac ctgcgtcaac gcctggctgc tgcctgaccc
    16861 ctgcgccagt tgttttagcg cctcttcaag ctgctgcata atcgcctgcg cgatccccgc
    16921 atccgccgct tcgctacgaa ttgccacgca aatatcgttc ggcgtggcgt tattaaaatc
    16981 gtcatgccag aaacctgtgg tatctaataa cgctttattg gcgggcgtac ccatcattac
    17041 ggaaacatca tcaacatttt ctgattcgct gagttttcgt gaaataatca ttaaactgac
    17101 cgaatcctga aaacaccctt ttttaataaa ggcgtggatc ataacaactc cttaaaaatt
    17161 gcacccatgg caattgcacg agcagtgtta atgtggcgat tacggtatcg atgacaacga
    17221 gtaaataagg tgatgaatat cacacaggga ttattaacaa aagaattcaa ataaccgcca
    17281 tgctattacg tgttattttt taataagcgt ggttgtttta aaaaccaggt taaaagcgag
    17341 cggattatca aataaattct aactatattt ttttgcctgt ctggatcaca taatccagat
    17401 attttccctg tatgttaatt gcagtcatgc ttcacaccgt cgttaaaaag gaagacagat
    17461 gaaaatcagt cgggaaacac tccaccagct aattgagaat aaactctgcc aggctgggtt
    17521 aaaacgtgag cacgctgcaa ccgtggctga agtattggtt tatgccgatg ccagagggat
    17581 ccactctcat ggcgcggtgc gcgtggaata ctacgcggaa cgcatttcaa aaggcggcac
    17641 caaccgcgaa ccggagtttc gtcttgagga aaccgggccg tgctcggcaa ttttacatgc
    17701 cgacaatgcc gccggacagg tcgcggcgaa aatgggtatg gaacatgcca tcaaaaccgc
    17761 ccagcaaaat ggcgttgcgg tggtcggtat cagccggatg ggtcacagcg gcgcaatctc
    17821 ttattttgtg cagcaggcag cccgcgccgg attaattggc atttcgatgt gccagtccga
    17881 tccgatggtg gtgccgtttg gcggcgcgga aatttactac ggtactaacc cactggcctt
    17941 tgccgcgccg ggagaaggcg acgagatcct tacctttgat atggcgacta ccgtacaggc
    18001 atggggaaaa gtcctcgacg cccgctcgcg taatatgtct atcccggata cctgggcggt
    18061 cgataaaaac ggtgcaccaa caaccgatcc gttcgcggta catgctctgc tccccgcagc
    18121 cgggccgaaa gggtatggcc tgatgatgat gattgacgtc ctctcaggcg tcttactcgg
    18181 cttaccgttc gggcgacagg ttagttcgat gtatgacgat ttacacgccg ggcgtaattt
    18241 ggggcaatta catgtagtta ttaacccgaa ctttttctcc tccagcgaat tattccgtca
    18301 acatcttagc cagaccatgc gcgaattaaa tgccattacc cccgcgcccg gttttaatca
    18361 ggtttattat cccggacagg atcaggatat taaacaacgc caagccgccg tcgaaggcat
    18421 cgaaattgtt gatgatattt accagtattt aatttccgac gcgctttata acacgtcata
    18481 cgaaacgaaa aatccctttg cgcaataatt attgagacag gactttctta tgattacaca
    18541 ttttcgtcaa accatagaag aagcgctgcc ctggctttcc tcttttggcg ctgacccagc
    18601 gggtgggatg acccgtttac tttattcgcc ggaatggctg gaaacccagc agcaattaaa
    18661 aaaaagaatg gccgcaagtg ggctggaaac acgtttcgat gaagtgggga atttatacgg
    18721 tcgcctgagt ggcaccgaat atccacagga agtgattctg agcggttcgc atatcgatac
    18781 cgtggttaac ggcggtaacc tcgacgggca attcggcgcg ctggcggcgc ggctggcaat
    18841 tgactggctg aaaactcaat acggcgcacc gctgcgtacg gttgaagtgg tggcgatggc
    18901 ggaagaagaa ggtagccgct tcccgtatgt tttctggggc agtaaaaata tctttgggct
    18961 ggcgaatcct gacgacgtgc ggaatatctg tgatgccaaa ggaaatagtt ttgtcgatgc
    19021 gatgaaggct tgcggattta ctctgccgga cgccccgcta actccgcgtc aggatattaa
    19081 agcctttgtc gaactgcata tcgaacaggg ctgtgtgctg gaaagtaatg ggcaatcaat
    19141 tggcgtggtg aatgcaattg tcgggcaacg tcgctatacg gtgacgctga acggcgaatc
    19201 aaaccatgca ggcaccacgc cgatgggtta tcgtcgtgat acggtttacg ctttcagtcg
    19261 catttgccat cagtcgatcg aaaaagcgaa aaagatgggc gatccgctgg ttctgacctt
    19321 tgggaaagta gagccgcgcc cgaatacggt gaatgtggtg ccgggtaaaa ccacgttcac
    19381 cattgattgt cgtcataccg acgccgccgt gctgcgtgat ttcacccaac agttagaaaa
    19441 cgacatgcgg gcgatttgcg atgaaatgga cattcgtatt gatatcgatt tatggatgga
    19501 cgaagaaccc gtgccgatga ataaggacct ggtcgccacc ctgacagaat tgtgtgaaag
    19561 tgaaaaactg aattaccggg tgatgcacag tggtgccggg cacgacgcgc aaattttcgc
    19621 gcctcgcgta ccaacctgca tgatttttat ccccagcatc aacgggatca gccataaccc
    19681 ggcggaacgc accaatatta ccgaccttgc cgaaggggtc aaaacgttgg cactcatgct
    19741 ttatcaactt gcctggcaga aataaggagt cataaatggg atatttaaat aacgtcaccg
    19801 gttaccgcga tgatttactg gctaaccgtg cgattgtgaa acacggtaat ttcgcactgt
    19861 taacaccaga cggtctggta aaaaatatta ttccgggctt tgaaaattgt gacgcgacaa
    19921 tcctctccac gccaaagctg ggtgcctctt ttgttgatta tctggtcaca ctgcatcaaa
    19981 acggcggcaa ccaacagggc ttcggtggcg aaggcattga aacgttcctg tatgtgatct
    20041 ctggaaatat cactgccaaa gccgaaggca aaacattttc cttaagcgaa ggtggctatc
    20101 tttattgccc gcctggctcc ttaatgacgt ttgttaacgc ccaggccgaa gacagccaaa
    20161 tctttttata taagcgccgc tatgttccgg tagaaggcta tgcaccgtgg ctggtttctg
    20221 gcaatgccag cgaactggaa cgcattcact atgaaggtat ggatgatgtg atcctgctgg
    20281 atttcctgcc caaagagtta ggttttgata tgaacatgca tatcctttct tttgcaccag
    20341 gtgccagcca cggttatatc gaaacacatg ttcaggaaca cggtgcctat attctttccg
    20401 gtcagggggt ttataacctc gacaataact ggatcccggt gaaaaaaggc gattacatct
    20461 ttatgggcgc ttattcttta caggctggtt atggcgtagg gcgtggtgaa gcgttcagct
    20521 atatttactc gaaagattgt aaccgcgacg tagagattta agtcatacca ctcgcctgat
    20581 tatttttagc tatgttggat tttgccacgg tttataccgt ggcttttttt gcaacatagc
    20641 ctgatgcgct tcgcttatca ggcctacatg atctctgcaa tatatagaat ttgcgcgatt
    20701 ttcaggaagg atatggtact catgccgcat ccggcatgag tactgcactc ttatgcgata
    20761 taaatcgcat ccgctttaaa ggtaagggtc agtttttaat tccctgacct attttaatgg
    20821 cgcaggcaat atttcgcgcg ctgttgaaga gattggtttc accgctggcg agcacttcgg
    20881 ctaaaggtgc cagacgtggc aaaatgctga ataccgcatc aatgccgtac tggtgcacca
    20941 cttccacgcc atcacccaat acgccagcaa tcccaatcac cggtacatta aactgcttcg
    21001 ccaccgacgc aacgcccagc ggcgctttac cgcctgccgt ttgcgagtca atgcgccctt
    21061 ccccggtgat caccagtgct gccccctgca ccgcctgctc caggttgacc gcccgcagta
    21121 caatctcaat acccggttta atatccgcat tgaggaatac cgccgccgca atccccatac
    21181 cgcccgcagc gccaccgccc gccatctggc agacattaat ttcagtttgc tgttgtaaaa
    21241 cacgggcgta attttgcagc ccctgttcaa gttcttcgac catctccggc gttgcccctt
    21301 tttgcgggcc aaacaccgcc gccgcgccgc gtgcccctac cagcgggtta tcaacgtcac
    21361 atgctacttc aatatggcag ttcgccagac gcggatcgca ttcctccatc tcaatgcttg
    21421 ccacgcgcgc taaattgccg ccgttagcca ccagcacctg accgtcggca tcaaggaaac
    21481 gcacaccgag cgcctgcgcc atgcccatac cgccgtcgac cgtcgcactg ccgccaatgc
    21541 cgagaataat atggcgaatg ccgttatcca gcgcatgacg aattaactcc cccgtaccaa
    21601 aactggaggc cagcaacgga ttacgctttt caggcgcgac cagcatcagg ccacttgctg
    21661 ccgccatctc aatcaccgcc gttttcccgt cgccggtaat gccataaaaa gcattcactt
    21721 tttcgcccat cggcccgcag acttcaagcg tcacgatgtt gccgcccgtc gcggcgacca
    21781 tcgcatccac cgtcccttcg ccgccatccg ctatcggcaa acagatatag ttcgcatcgg
    21841 gaaagagggt cgaaaacccg gctttaattg cctgacaaca tttttctgca ctcaagctct
    21901 ctttaaaaga gtctggcgca atgacaatct tcattatcaa tcctttttca acaaattatt
    21961 cgatgcgttc ccacggaata aggttatcca taagaacagc cagtaaaatc ccgaccaata
    22021 agccgttact gagcagagga cgaagcgtta atggaaggtc ttgcagatac acaggcggta
    22081 atgccattaa aaatatgccg acaaataacg gcaatgcgag tcgataaata ttgcgagcgg
    22141 taaacgttat ttgctggcta aacaccagcg cggaaaagag taaaggtaga taagaaacca
    22201 gcatgaccgc actactcacg ggtaaaggga tactgcaaaa cagtcgcgtg agtgccggaa
    22261 ccagcgccac cagcaggcaa ataacgctgc cataaataaa cgaacgccgc gtgtaatcgc
    22321 cagtctgggt taataaacca atggatgaaa caaacggtga aaatggaatt actgccagcg
    22381 gcacggttat cagtgtcata aatccggtcg ccacaaagct acgacgataa cgcgtattcc
    22441 ccgcgccctg ctgcggataa aaaacatccg tgccccgaat cgcaccgtag gtattgctga
    22501 tattcaccag acctgtaatc acctccgtca gaataatccc cggcgacaaa gcaccgccac
    22561 tgccgagcgg gaaccactgc cagtgcaact caccggagag cgaatgcgaa gaaggaaagc
    22621 aaaagtacca caacaaccag ccggttatgg tgccgaccag caggccataa cgggcaaaac
    22681 gttgcggcag aaagataatc atcgccagta ccaggcacat caccgccacc gagagcgcga
    22741 acggcggtaa ctgaatttta aaattcgggt cggctatgcc aaacggcagc ccgagcatac
    22801 ctttgaaaaa gatagtggtc agctgcgcgc ccagcatcaa cataaacaag accatcaccg
    22861 acggcgtaaa taaccgcgcc aggcgatgac ctaatccgct aaaaccaatc aacatcgtca
    22921 gcacgccgga gagtgcaatc cccaccgcca ggctggtagc gatatcgttg atcggtgtcc
    22981 cgcgcgatgc ttcgccaagg gtgatagtta ggatggttcc ccaccacagg ccccccggcc
    23041 cttccataat agcgcgacga tgaccgcaaa acgcctgagc gaagcaggcc agtgcggtag
    23101 caagaaaagc atattgcgtg agcgtaagca ggctactttg cggcaactga aaagcagaaa
    23161 gtagcgtagg aggaaccaca accgtgttgc aaaaaataaa gaaaaaccac tgaaatcctg
    23221 ataacaggct ttcgcggcta actgcaaaat tgaacataaa cacaccttaa ccggagaatg
    23281 cccgccgcaa ggacgggcat tgcaggggcc agattactgc tgatgtttaa ggataaattg
    23341 acctttcggc gcaacaggga agccctgttc gatgtcgtaa atcacatcac cacgtaagat
    23401 ggttttcgtg atacgcgcgc caatggtacg gccaacatac gggctgactt tgtggcgata
    23461 ttccagatcg tcattggtaa gaacatagct gctattcggc tgaatgaaga cgaagtcggc
    23521 atcttttcct ggggcgatac ggcctttttg ctgcagacca aaaatatctg ctgcgttagt
    23581 cgccattaat ttgccgaaca ttggcagaga cattccgcgt ttctgtaccg cttcatcgaa
    23641 catcacgtcc atacagtttt gcagaccggc aataccgccc catgcttcca tgatgttgcc
    23701 ggctttcatt tccggagggc atggtgagtg gtcggaaacc aggcggtcga tttcaccgtt
    23761 aaacagtttt tcccacatgc ctttctagtt ttccagatcg cggatcggcg gtgaacactt
    23821 cgccagagta ccgatttctt cgaactgatc ggtatccagt acgaagtaat gcgggcagga
    23881 ttcacaagta acgtcctgac cttcctgacg tgcacgagtc acttcttcaa cgccttccgg
    23941 gctgctgata tggcaaacgt gcagacggca accggcaact ttcgccagat acagcacgcg
    24001 gcgaatcgct tccacttcgg taaataccgg acgcgaagcc acatagtcat gggcagttac
    24061 gcgaccttca cgtttcgctt cttcgcccag tgcgtcacag atcagcgcgt tttcgcagtg
    24121 caccagcacc ggctgcccca gttcgcccag cttctgcgca cctttgaaga actgccagtc
    24181 gtttacatca cggaagtcgt tgtcgatacc gcgatcgcca caggtcgcaa cgaagcattt
    24241 gaagccgaca acgcccactt catccagctc atgcagacgg tcgatgttgt aagacaccag
    24301 gccaccgagt tgtgccgcat cgatagtcag cttgccttta gcggcatcga acttcagttc
    24361 aattgaagcg cggtcaaccg ttgcaggcag ctggttgagc ggcatttcga tcatggtggt
    24421 gataccacct tttgccgctg cgcgagtacc ggtttcataa ccttcccagt ggctacgacc
    24481 cggttcagaa atatgggtgt gcgcatcaac catgcccggc gaaaccacca gaccagacgc
    24541 atccataact tcttttgcat cgcccagatc ctgaccgata gcagcaattt tgccgccttt
    24601 aacggcgata tctacaacgc gagcttcgtt ttctaaaata acggtgccgt ttttaatgat
    24661 taaatcaaaa gacataacaa actccttgta aaatacagat tggtttttaa ctcaagcaaa
    24721 ggcgacgatg acgccgacaa accatgaaac acgcgataac ggttccatta agggaataaa
    24781 cttaccgcca agagataaaa taacggccac cagagttact gaaaacgcgg tgaggttaaa
    24841 accgttatcg taatatttat aatcgccagg tgcggtgtac agttcatcaa gattaatttg
    24901 cccacgcatc accacaaaat aatgcgccat catgacacca attaccggac caagcattcc
    24961 gccgataata tcgaggaaca gataaatact gtcctgattt tccattaatt tccacgggca
    25021 gatcagcagg ctgataatac tggcaatcag tacgccgttt ttataggtca gttttgtcgg
    25081 tgcaatggca gcaatctgat aaccagccgg aataatatta ccagtggcgt tagtggagat
    25141 agtcgtcatc agaataacca gtaccgcaaa gaacgaggca aacaggctgt cccaacgctg
    25201 aacaatatcc agcacgttcc aggtatcagc gccgtaatga atactggctc cggcaataat
    25261 acataccccc gcgaccgcaa acagaatata ggccacaact aaacccagcg tttgccccag
    25321 cgcttgctca cgaaacgagt gggcgttttg cgtaaagtcg gatgcgctca ccgccggtgc
    25381 cgcccagacc gcaactaccg cgttaatcac caccaggaac aagaatccac tgttttctgc
    25441 tttctgaata ccgctcggaa tgtagtcaaa gattggaccg agcccgacca gcgaaatcgc
    25501 ccaaatcgcc ataccgccga aaacgatata gatgcacggg ttaagaatgg cagtgaattt
    25561 atttaaaact ttgccaccgc caaaacctat accaacgttg accagccaga agagtaagaa
    25621 agtaattaag cccggtagag aaaggcctaa cagagtgaaa tcaccaccga gagtcaaaaa
    25681 tcccggccag attttgccaa tcagaatcaa gcaggccagt gaccccgcgt aacattgcag
    25741 gccaaaccac ataatggcag caataccgcc tcttaataat ccgggaaaca gtgcgccacg
    25801 tacgccgtaa gaagcacgca ggatcatggc aaacggtacg ccgtatttac tgcccgcagc
    25861 gccgtttaat accattaccg cggcaatgaa aaaggcgctg aggataattg ccagcataat
    25921 actaaaggta gacaagccga gaataaaaaa gccgccgacc atcacataat tgggaacgtt
    25981 atgaaccgaa cccatccata aggtaaaata gttaaatgtt ttccaggtcc gctggctttg
    26041 cgttttcggc aatagatctt cgctatagcc gcgttgctgg aatagttttc tctgatgttc
    26101 cataaatccc tcttaatttc acgtatcgaa taacgttgtt ttaatctagc tatgaggaaa
    26161 aaataaaata aaaatattat ttttaatact gaagcaacat ctttggctgc tttctttttt
    26221 aatattgctg gccgattcat tttttgatat cggccatttt tattgcgggt atcaggccag
    26281 tttatggtta gccattaatt ccagcgcctg cactaacgca gagtgatcca actggctgcc
    26341 accgtttgcc gcgcaggtat taaataactc ctggcaggtc gcagtgtttg gcaggttcag
    26401 agccagtgcc ttcgcacttt gcagtgccag gttgagatct ttctggtgca gcgcaatttt
    26461 gaaacctgga ttaaaggtgc gtttaatcat gcgctcaccg tgaacttcca gaatacgtga
    26521 ggaagcaaag ccgcccatca gcgcctggcg cacacgtacc gggtctgcac cggcttttga
    26581 agcaaacagc agagcttcag agaccgcttc aatattgagc gctacgataa tctgatttgc
    26641 cactttgcag gtttgaccgt cgccgttacc gcccacaagg gtgatatttt taccgagcag
    26701 ttcaaacagc ggtttaacac gttcaaatac cgcttcgtca ccgccaacca taattgacaa
    26761 cgtcccttca cgcgcgccga tttcgccgcc ggagactggc gcatcgagat aatcaccacc
    26821 cagttcattc acctgacgag cgaaacgttt agtttcaatc ggtgaaatgg agctcatatc
    26881 aacaatggtt ttgcccttca gcgaggcttt ggtacaacca ttttcaccaa acagaacttc
    26941 ttcaacctga ggtgtgtccg gcaccataat aaaaatgatg tccgctgctt ccgttacctg
    27001 gcgcgcggtg tcgacattaa cagcaccgag tgacagtaat tcatcagcaa ccggtccaat
    27061 ggtcgtgaca tgtaattgat gaccggcacg cgccagatta atggccatcg gagtacccat
    27121 aatgcctaag ccaataaatc ccagtttcat aattaacctc tttttaaatg tcgctattcc
    27181 tgaattcaga caacacgatc ccctgccaac ttagcagcct aaaaaagcat tgtctgaata
    27241 gcgttacgtt ttaacggtac ggatccatcc agcgtaaacc ggcttccgtg gtggtttggg
    27301 gtttatattc acacccaacc caaccgttgt agtcagaatt ttcgattacc ttaaagagat
    27361 aatcataatt aatttctccg gtccccggtt cgccgcgatg cggattatcg gcaatttgca
    27421 ggtgaccaat tttatcagcc cattgcgtca tagtgttggt taattcacct tccatccgct
    27481 gcatgtgata aatgtcatac tgaattttca aattgcagca accaacatca tcaatcaatt
    27541 tcagcgcctg ccgagttccg gtgagatgga aaccaggaat atcaaaatgg ttaataggtt
    27601 caatcagtaa taaaatatct tctttcatca gcatattcgc ggcataacgc aggttttcta
    27661 caagcgttgc gtgaatctgt tcactgctga aaccagccgg cgttttaccg accagacagt
    27721 taatattttt attacccagc gcacgggcat aacgaatcgc tgctgctacg ccatcccgaa
    27781 actcttcttc acggccagga atacaggcaa taccgcgctc ccccgccgcc cagtcacccg
    27841 ccggtaaatt gtgcagcgta tgttcgagtt tattactcgc cagcacctgt tttaattctt
    27901 caatgtcgta gtcataagga aacataaatt caacgccgcg aaaaccacac tgcgcagctt
    27961 tctcaaaacg ggcgagaaaa tcatattcac caaataacat cgataaatta gcagagaaac
    28021 gtaacattat ttatctccct tattcatagt gcatgaagca ggtttcagtc ggtgcatccg
    28081 ctgcgttatc ggcgatatct tcaaattcca tgacgttatc cagttcgctg cccatcgaaa
    28141 tatttgtcac acgctcgaga ataacttcca cgactaccgg tacccgatat tgcgccatta
    28201 aggctttcgc ctgttcaaag gccggtgcaa tatcttccgg tttgaagacg cgaatagctt
    28261 tacaacctaa accttccgct acttttacgt ggtcgacgcc gtaaccgttc acttcgctgg
    28321 agttgatatt ctcgaaagcg agttgcacgc agtagtccat gtcaaaggcg cgttgtgact
    28381 gacgaatcag gccgagatag gcgttattca ccagcacatg gatgtacgga atgttgaact
    28441 gcgcgccaac ggctaactct tcaatcagga actggaagtc gaagtcaccg gaaatcgcca
    28501 ccacattgcg tttcggatca gcggcacaaa cccccagcgc cgccggaatc gtccagccta
    28561 acggaccagc ctgaccacag ttgatccagt ggcggtcttt aaagacatgc agcatttgtg
    28621 ccgcagcgat ttgtgacagg ccaatggtgg tgacataaca aacatcgcga ccaaaggctt
    28681 tgttcatctc ttcatacacg cgctgcggtt tcaccggcac gttgtcgaaa tgggttttgc
    28741 gcagcaaagt gcgtttgcgc tgctggcaat cggcgaccca ttctttacga cacggcagac
    28801 gacccgcttt ttgcatctcc tgcgccactt caaccagcag tgtcagcgcc gctttagcat
    28861 cagagacaat gccgagatcc ggacacagca cgcggccaat ttgcgtcggc tcaatatcga
    28921 tatgaacgat tttgcgccct tcggtgtatt tctctaccga accggtatga cggttagcaa
    28981 aacggttacc gataccaaac accatgtcgg acgccagcaa cgttgcgtta ccgtaacgat
    29041 gcgcggtttg cagaccgacc atcccggcca tcagttcatg atcgtccggg atacagcccc
    29101 agcccatcag cgttgggatc accggaacgc tggtcagttc agcaaactgt tgtaacagtg
    29161 cagctgcgtc agcattgatt accccgcccc cggcaacaat caccggacgt tcggcctgga
    29221 ttaacatttc tacagctttt tcgatctgca tacggctggc agcaggtttg tagaccggca
    29281 gcggttcgta catgtcagga tcaaactcga tttccgcaac ctgaacgtcg aacggtaaat
    29341 ccaccagtac cggacccgga cgaccagaac gcatcaggtg aaatgcctgt tgcagcacgc
    29401 gaggcaccag cgccgcttca cgaactgtaa ccgccatttt gctgaccggt ttagcaattg
    29461 cttcaatatc tacggcctga aaatcttctt tatgcagacg ggcgcgcggt gcctggccgg
    29521 taatgcacag aataggaatg gaatcagcag aagcggaata gagcgcggtg atcatgtccg
    29581 tgcccgcagg accggaagtc cccagacata cgccgatatt ccctgccgtt gcgcgggtat
    29641 aaccttccgc catgtgcgaa gcaccttcca catgacgcgc cagaatgtga cgaataccgc
    29701 cgtgcttacg catcgctgag tagaacggat tgattgcagc tcccggaacg ccgaaggcgg
    29761 tggtgatacc ttctttctcc agcacataca ttgccgcgtc aacggctctc atttttgcca
    29821 ttttattcct acctctatta attggaaaaa ttttccaact ttcaaatttt gtcaaaaaaa
    29881 cccgctccgc agagcgggat gcgacagaca ctatggatgt gctttcagtc ccaacgccgt
    29941 gctgatatcg cgggcggtgt ctctgaccag ctcaccctga ctgacaaaac gatcttctgt
    30001 cagtcttgat gaaggcccgg agatggagat agcggcaaca acgctaccta catcatcgta
    30061 aattgctgaa gctatgcaat tcagacctac aacatgctct tctttatcta cggtatagcc
    30121 cagttcacgc gcttgttcca ggtccttcag cagggtgggc atatccacaa gcgtagttgg
    30181 cgtaaactgc tgcaaaccgg tttgcagaat gatgctcatc aactcctctt ccgccagcgg
    30241 ataaagcagc gctttgcccg caccggaagc atgcagtggc agacgactgc ccagtggcgc
    30301 gcacatcctg accatcgatt tacactctaa ctgaccaatt aataccgctt cattgccgtt
    30361 acggatcgcg acattgaccg tttcgccgga aagtaacatc aggcggcgca taaacggccc
    30421 ggcgacggag aggacatcgc ggttatggat gtacgccgca ccgacgttaa agacacctaa
    30481 tcctatatgc caccagccta actgactgtc ctgatagaca aaatccgctg cctgtaaaac
    30541 cttcagcaag cgaaaggtcg tggagagcgg caaatccaga ttgagagaaa tatcgctaac
    30601 cgacgaactt cccccacttt tttccagata ttgcagaatc gcaattcccc gctctaatgc
    30661 ctgtgcgccc ttctgtgcca caggctccgc ctgtcctggc ctgccgcgcc gtctaacttc
    30721 cgtcatacct acgttaactc cgacctacaa gtgagcataa acaatgaaca attagtttat
    30781 cttatgcaag tcggttgcag gcgtcacgca aaacagagtt cctgttcagg aatactttca
    30841 acatcacagt tgtcactgcc gccgcgatcg atggtcagaa aatcggtgac gcgctgccag
    30901 gcgaaaagtg ggtgatgcca gacgttacga tggtaattca ctccctgttc gccgttggta
    30961 ataaacgccc gcagggttga caggtctggt ttgtcgtcac ctaacgctac gaccaccaca
    31021 aacacctcac ctttcatcgg gataaaggcc tgagtaccca gcggatgacg ttcgagttcg
    31081 tgaatggtca gcggcagatt cgccggttgc gctcggttaa tgctgataag cgtacggtct
    31141 tgctcaagaa tctcaaccag cgccaaatcg tggtaacgct ccaccaggcc attgttaata
    31201 tggaaaaaat cccgttgctg cgtttcgatt acgtcgccat aagcactaaa ggcttcctga
    31261 cttaacggta atacctgaag tttcatcttt ttgtccttgt aacataattc cgttcaacca
    31321 gcgccgaccg gtcagcttgg tttcaataac aggcaatcta aaaccttaag agaacaacat
    31381 caacacaaaa cttggaatat ttttccaaaa ctagatttag tccacaaatt gaacagggtt
    31441 atcatgtaaa aatactgata aatcagagga taagaaaatc acgcaacttg cggatttaaa
    31501 aggggatacc tgaagatgtt cgatccagaa accttgcgga ctttcattgc ggttgctgaa
    31561 acaggaagtt tttcaaaagc ggcagaacga ttatgtaaaa ccacggcgac gatcagttat
    31621 cgcattaaac ttctggaaga gaataccgga gtggcgctgt tttttcgcac gactcgtagc
    31681 gtaacgttga cagcggctgg cgagcatcta ctttcccagg ccagagactg gctgagctgg
    31741 ctggaaagta tgcccagcga gctgcaacag gtgaatgatg gcgtggaacg ccaggtgaat
    31801 attgtcatca acaacctgct ctacaacccc caggccgtcg cccagttgct ggcgtggctg
    31861 aatgaacgtt acccctttac ccagtttcac atctcccgac aaatctatat gggcgtctgg
    31921 gactcgctat tgtacgaagg tttttcactg gctatcggcg tcacgggaac cgaagcgctg
    31981 gcaaatacct ttagtcttga tcccttagga tcggtgcaat ggcgatttgt catggcggcg
    32041 gatcatccgc tggcgaacgt tgaagagccg ctaacagaag cgcagttgcg gcgctttccg
    32101 gcggtcaata ttgaagacag cgcccgcacc ttaaccaaac gcgtcgcctg gcgattgcca
    32161 gggcaaaaag agattattgt tcctgatatg gaaacgaaaa tcgccgccca tctggcgggc
    32221 gttggcattg gttttttgcc aaaatcgctt tgccagtcaa tgatcgataa tcaacaactg
    32281 gtcagccggg taatcccaac gatgcgccct ccttcgccat tgagtctggc atggcgcaaa
    32341 tttggcagcg gcaaagcggt agaagatatt gtgaccttgt ttacccagcg caggccggaa
    32401 atcagcggat ttttagaaat tttcggcaac ccacgcagtt aaaaatcaag tttccttgcg
    32461 cgttggcgcg taaaatagcg cgcaagctga tttgaggttc acttttaact atgcaagaga
    32521 gacacacgga acaggactat cgtgccctgc tgattgctga tacgcccatt attgatgttc
    32581 gcgcccctat cgagtttgag caaggcgcaa tgcccgccgc tatcaatctg ccgttaatga
    32641 ataacgatga acgcgccgcc gttggcacct gctataaaca gcaaggctca gacgcagcgc
    32701 tggcgctggg acataaactg gttgcgggtg aaattcgtca gcagcgcatg gacgcctggc
    32761 gggcagcctg cctgcaaaat ccgcaaggta ttctctgctg cgcccgtggc ggtcagcgct
    32821 cacatattgt gcaaagctgg ttgcatgcag cggggattga ttatccgctg gtggaaggcg
    32881 gttataaggc actgcgccag accgcgattc aggcgactat tgaactggca caaaaaccga
    32941 tagtgctgat tggcggttgt accggcagcg gtaaaacgct gttagtgcag caacagccga
    33001 acggtgttga tctggaaggg ttggcgcgtc atcgcggttc ggcgtttggt cgcacgttac
    33061 aaccacaact tagccaggcg agttttgaaa acctgctggc tgccgaaatg ctaaaaaccg
    33121 acgcccgtca gaatttgcgc ctgtgggtgc tggaagacga aagccggatg atcggttcga
    33181 atcacctgcc ggaatgcctg cgcgagcgaa tgactcaggc ggcgattgcg gtggtagaag
    33241 atccgtttga gatccgtctt gagcgcctga acgaagagta tttcttgcgt atgcatcatg
    33301 attttaccca cgcgtacggc gacgaacagg gctggcagga atattgcgaa tacctgcatc
    33361 acggactttc ggctattaag cgtcggctgg ggctacagcg atataacgaa ctggctgcaa
    33421 ggctggatgc agcactgaca acgcaactca ccaccggcag caccgacggt catctggcct
    33481 ggctggtgcc gttacttgaa gaatattacg acccgatgta tcgctatcag cttgagaaaa
    33541 aagcggaaaa agttgtcttt cgcggtgagt gggcggaagt ggcggaatgg gttaaggcgc
    33601 agtaatgagg gacgtatttg tattagtcgc gacttgatct gacacatggc cttgaaaggt
    33661 tgagagttac cggttttgat atgagtgtcg aatccttatc caaaacaaga ggtaactctc
    33721 atgcttcata ctgccaatcc cgtcatcaaa cacaaagccg gtttgctcaa tctggctgaa
    33781 gaactcagca acgtatcaaa agcctgtaaa atcatgggcg tttcgcgtga tacgttttat
    33841 cgttaccgcg aactggtcgc tgaaggcggt gtggatgcgc agattaatcg tagtcgccgc
    33901 gctcctaacc ttaagaaccg taccgatgag gcaactgaac aggctgttgt tgattacgcc
    33961 gtcgctttcc cgacacatgg tcagcaccgg gccagcaacg aactgcgtaa acagggggtt
    34021 tttatctccg acagtggtgt ccgttctgtc tggttactcc ataaccttga gaatctcaaa
    34081 agacgatact gaacaaattt tatcaggtga cgttccgtaa aaagttgtat ggagttctcg
    34141 atgcagtcgg cgcgactatc agtacccccg ctgaaccctg gactatcggg ccgccgtact
    34201 ggggcatgtc accctggcgt gccgctggtt ttccgctaat ggtttatttc ctctttcttt
    34261 cccggcaaga atttatttta atccggaagg attatttatt ttcagatagg gactcacatt
    34321 aaacacgtaa cattttaatt aatgcatggc tgaatatgta ttacacaatg tcagatatcg
    34381 taaaagacca gtgcaattct atgtaaactc tctgactgtt cattttattt gttgtttcag
    34441 ggtcggaact caccctcaat aaacaactga atacgagtag atattcatcg tctgagctat
    34501 atggctttac acaatagccg acatgtcttt tctcagtcag gaattttact tttcctcact
    34561 acgaagttga cgacttcgat atgggataga ctcttaattc aagcaattac tctgcacaag
    34621 catcaggcaa tccagctcca atatttgtta taaaataatg cgtaacatta atttcaccaa
    34681 caaaaaaagg tttacaccat ccccttgccc tggtatctta cca
//
LOCUS       DBFIFI010000029        33699 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.029, whole genome shotgun sequence.
ACCESSION   DBFIFI010000029 DBFIFI010000000
VERSION     DBFIFI010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33699
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.029"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(271..1128)
                     /gene="repA"
                     /locus_tag="N7B39_001358"
     CDS             complement(271..1128)
                     /gene="repA"
                     /locus_tag="N7B39_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HGU2421367.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPRKGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESGAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTLALFAALDV
                     SEDAVAAARRSRVEWENRQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDAGRERQDIVTLVKRQLTREIAEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            complement(1121..1195)
                     /gene="tap"
                     /locus_tag="N7B39_001359"
     CDS             complement(1121..1195)
                     /gene="tap"
                     /locus_tag="N7B39_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HGU2421368.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            complement(1420..1680)
                     /locus_tag="N7B39_001360"
     CDS             complement(1420..1680)
                     /locus_tag="N7B39_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HGU2421369.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            complement(1925..2053)
                     /locus_tag="N7B39_001361"
                     /pseudo
     CDS             complement(1925..2053)
                     /locus_tag="N7B39_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300273.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
     gene            complement(2124..3119)
                     /locus_tag="N7B39_001362"
     CDS             complement(2124..3119)
                     /locus_tag="N7B39_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2421370.1"
                     /translation="MSWFSDIYGFEESTYARTQANFYIDGPLLHTRGQSSRTFHAGIL
                     TLPSLAEIRKAVCSLEVSRGEKIKLSSLVTDAYELHRCPEANRAIIQVASQFNLLEMP
                     NEHTTPEKGITNYQYDYTQGPACAMACAAATVFRNYLVPVGSQRGQTQNCQLNALADM
                     DRAIGIRGIRMQNGYALLQPDTVLAISKHIEAMDELSRDEVRQKLRVGVHSDTEVTIP
                     GVPKEQRVTQVLCAALPVAYHYSPRRDWGPFATLVLEACYEATLLAAVLNYHDTGNPR
                     VYLTLVGGGAFGNDLSWIVSALRRALNLVSNHPLDVRLVNNRKVPVEIESLIREF"
     gene            complement(3225..3509)
                     /locus_tag="N7B39_001363"
     CDS             complement(3225..3509)
                     /locus_tag="N7B39_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2421371.1"
                     /translation="MNGFRNRSRSSQVWRYRCVGNRAVRLEVRELWAEAADVWWLAAH
                     IAPRTDWQQFAWQRAEYCQRRSRGKAGHPPRSSGGSGCPAKIKEHTRQVG"
     gene            complement(3699..4430)
                     /gene="traX"
                     /locus_tag="N7B39_001364"
     CDS             complement(3699..4430)
                     /gene="traX"
                     /locus_tag="N7B39_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008455316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     acetylase TraX"
                     /protein_id="HGU2421372.1"
                     /translation="MNQGKSVAVQPVRPVLNFSALQRDVIRMVAFVAMVGDHIATAFQ
                     LDMPLLNMGGRCAFPLFALVCGCNMAGKTLRQHSLNRLWLMALLAQPGYWLAFRDVGL
                     AWWQLNILFTFAVVMQVALFLQSATARNGVAAFTVLMGYTPFSSASYGIPGLLMLAGA
                     LLVWQVRGTLRPALFAVWLLLVALLNARHGDVMMFSGVILTLTVLFCVHGLVPASGRR
                     LQAGRWFAPAYAIHLFFIGFLVRVL"
     gene            complement(4427..4993)
                     /locus_tag="N7B39_001365"
     CDS             complement(4427..4993)
                     /locus_tag="N7B39_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HGU2421373.1"
                     /translation="MFIAFIIIVILLGGFVLLLRQAGRAAHPLLQSLRSRGIRPGAAE
                     LLLCSRPSFVSAGRLMTEREQRFLRRLDRVTDTRHWRLCPQVRVADIVRVAPDRKSGS
                     REWWQLFRLVSQWHCDVVITDRAGRIIAAVELDDRSHQEPKRQRRDLLLEEVLKQAGI
                     PLLRGDNEQQLAARVRAHLCAQRPEAAA"
     gene            complement(5011..10305)
                     /gene="traI"
                     /locus_tag="N7B39_001366"
     CDS             complement(5011..10305)
                     /gene="traI"
                     /locus_tag="N7B39_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HGU2421374.1"
                     /translation="MLSISSIKGDAGYYSHEDNYYASGSLDSRWMGEGAEKLGLKGEV
                     ASADMDAVRQGKLPDGSDLSRMVNGVNKHRSGYDLTFSAPKSVSVMALVGEDRRFIEA
                     HNRAVAVVMQEVEKLVSARITEEGKTETVLTSNMVAALYNHDTSRDLDPQLHTHALVF
                     NATFADEKWRSLASDTRMKTGFSENLYATKIALGNLYRSALREDVESMGFETVAAGKN
                     GLWELKDVPVDVFSSRSQTILEAAGPDASAKSRDVAALDTRQAKAWADPELLKADWRR
                     RLADEKFDIDGYIRQAQARVEVPGAVAGRHSGIHAPDLSGGASFGEIADEQVRKAVSD
                     TISALSEKKVQFTWSEMLAGTVNRLPSAPGLFEQARAGIDAAIEGQRLIPLDREKGIF
                     TSDIHLLNELSVHQLARTAIQEQTVLLFPERAQERDIPAGDAVSVLTQDKSPVAILSG
                     RGGAQALREHTEDVAMMARSQGREVMVIAADGRSGQFMSESPHLAGHVMLRSQMNADT
                     VLPVQGTVIVDRAERLSLKETVLLQEKALSAGAQLIFMDTENRQGTGNALSVLKEAGI
                     PQYRFYGTQLPEVRLVSEADKRSRYGQLAKDYVRLSSEGRDVVAQVTGAREQQQLTDV
                     IRDARREAGELGREQMTIRVLEPVWLDSKTRHQRDNYRAGMVMEQWDAEKKMMTRHTI
                     DRVAEATNSLVLLGEDGSRLTLKVSQLDGSWSLYRSRTLAVSEGDRVRALGRELKGAI
                     KAKEQFTVTALENGAIRLRSGERELRLPTERAVKLAHDYVEGTGAGTSASRTVLAAVG
                     PRALNKQVLNALAQSGGDIRIYTPLEARQAARKVESVSAVRLASDQLRQSTGEENLDI
                     AIQASRERLMSDAEQAVSLAIPRAQQGQVHLSEITLLSEAVKSGQPLADVRTEIARQV
                     SSGELIQLDSVAGAGNRILVPRVAYEMEKSIIRHIAEGKDAVQPLMSLTPASVLSGLT
                     AGQREATRTVLENTDRFMAIQGYAGVGKTTQFRAVMGALNTLSESVRPQVIGLGPTHR
                     AVHEMREAGVDARTLASFLSETRQAIQAGETPDFRNVLFLTDESSMVGNRDMAELYQL
                     VAAGGGRMVSSGDTAQLQAVSTGQPFRLVQQRSAIDTVVMQEIVRQTPALRPAIESII
                     AGQVETSLRQVDDVSPQQVPRQAGAWVPEKSVMEIRKPEKEPEQGRTAAQEALKPEQL
                     SPARTDIIAAIRDDWMGRTPEAQQQTLIMAELNADRHAINEAIHVARHEKGDTGAEER
                     TFTVLEPLRVPDNALRAAETFAEYTGAVAMMNERYWTVAEVDTQDAVVTLRNADGESV
                     LLSPQQNTAQDISLFTPRDLTISQGDRVRFTRSDTDRGYVANSLWEVAGFTDEGAIRF
                     RQGDQEKIVDPQAMTEDRHIDLAYALTVYGVQGASERFAIALTGTEGGRKRMASLEST
                     YVTLSRAKEHVQVYTDNLEDWQQQVRQSDGGKTAHDLLHEKSDRESDTGNRLLATASR
                     LDKTALGRRVLAENGLEGETMARFIAAGKKYPSPYVALPVWTRHGKEAGALLTEIRIE
                     DDGMRVVLSEESRLRGGEDAQFAGLQASRNGQTLIADSADAALRLAQENPESGVVIRL
                     HGEERLLNAARLTGGRITEPDELSRALRNVADAETAAKAEDPITLPPDEQQKLAEVQE
                     KAARELAEQARRELLSVAPQKDEDKRDPLLSADDERRLRDGTERESRELDEAVREAVA
                     GGRGARQQVREQMQRTEREWVVKMPEKEIELEKTLGGD"
     gene            complement(10305..12536)
                     /gene="traD"
                     /locus_tag="N7B39_001367"
     CDS             complement(10305..12536)
                     /gene="traD"
                     /locus_tag="N7B39_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HGU2421375.1"
                     /translation="MSLNPRDLTQGGQVAFMRLKMFLQINNLISYYVIMGTVLFGIAV
                     LLMRISIQNLTNGIIYWFVRMISPFTEKMVSQPVYTIRYYEHKLQYSARQILNDDYTV
                     YCGQLLKQELIIAGCAALLVAFVATFAVYWYIGRTGRQQSEDEIIGGRALSEAPKDVA
                     RMMKKRGEASDIRIDDLPLKLDSEIQNMSMHGTVSTGKSTLMRKIMKQLRDRGDLVII
                     YDKGCTFIEDFYDESRDKVLNALDARCPNWDLWEECRTISELENASATLIPASSGEDP
                     FWQGSARTIFAEGAERMRRDEDRSYNKFLRTLLAIQLDQLRTFLAGTPASTLVDGKIE
                     KTAISIRSVLTNYVKAMRYLQGIDRPGREKFTIREWMKGQADKSKNGWLFITSDEQNH
                     ESLKPLISLWLSIAATSLLAMGPNRQRRVWFFYDELASLHKLPTLPRIISEARKFGGC
                     FVLGFQSFAQLEEIYGPKAAAALFDLLNTKFFFRSPSAQIAKFVEEDIGETWRKKFSE
                     QTSFGHEQVRDGISFGKEEERVSIVSYSDVQSLNDLQCFVTLPGSYPVVKLTMKYEAM
                     PKVADSLLLRDVQTSLDQMLEDEITRRTEEERHKFAGLFEGASLIQAVPESNLSNTPP
                     PSAGRVAAVLAETNTPPETRTLQTENAAGDADTKNTGSTAVAAEGGSTSTGGTERDIE
                     QDLPKDVPPGLNSDGEVVDSEAYEDWVQASHQTRDMTRREEVNINHATDKARDFDDDR
                     EVF"
     gene            complement(12680..13417)
                     /gene="traT"
                     /locus_tag="N7B39_001368"
     CDS             complement(12680..13417)
                     /gene="traT"
                     /locus_tag="N7B39_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001485398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer complement resistance protein
                     TraT"
                     /protein_id="HGU2421376.1"
                     /translation="MKQNIKLMAMTAILSSTLVLSGCGAMSTAIKKRNLEVKTQMSET
                     IWLEPSSQKTVYLQIKNTSDKDMSGLQAKVTKALQDKGYTVTSAPESAYYWIQANVLK
                     ADKMDLRTAQGFLSQGYQGAAMGAALGAGITAYNSNSAGATLGVGLAAGLVGMAADAM
                     VEDINYTMVTDIQISEKTNATVQTDNVAALKQGTSGYKVQTSTQTGNQHKYQTRIVSS
                     ANKVNLKFEEAKPVLEDQLAKSVANIL"
     gene            complement(13585..14160)
                     /locus_tag="N7B39_001369"
     CDS             complement(13585..14160)
                     /locus_tag="N7B39_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraS"
                     /protein_id="HGU2421377.1"
                     /translation="MKVTTDDLKKDVEMLFDDLKRNYEIPSSSSSIKFLFKIIAVALV
                     VQFFLSALDFYIWGEEYRGGCREAIIVYFIGFLGVLLLPSFILIIVYHNPIMMISCLS
                     DEARRKSVLLNIAKAKLQYVLYFAIVTNLLVGICFALNESAMIAFMGGSWFVTVIIST
                     SIYNMMMAPYVTPAVTSSLLKMKELLSESRN"
     gene            complement(14170..17052)
                     /gene="traG"
                     /locus_tag="N7B39_001370"
     CDS             complement(14170..17052)
                     /gene="traG"
                     /locus_tag="N7B39_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000963403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating-pair stabilization
                     protein TraG"
                     /protein_id="HGU2421378.1"
                     /translation="MAIDTIYTIAGGAWFQDTLNGVASFLDSRAGDALIAMATAVSVI
                     VGAATYIRTRNIMDLVKWAGFYVLVIAVLIGDKRSVQIIDLSEPARIYQVDNVPAGLA
                     APASMITRIGAGMAQTYDTIFARPDALTYSKTGMLFGAQLAAGSSDFRFSEPEIQRMF
                     SDYVHNCVVGDIMLNNKYTIGELMNAADPYALIFSRPSPLRGLYDKNRNFLTCEQAAT
                     RINTDSGDISGANTSPFLQQILNTLHGFTDQVFGQTNGAQSALFTEMLGDSYNYFHRT
                     SLTSTEIIRKNVIMNGLRSGLESFSALSGDTAGLVNVATQTSMAKMRLSQATSASIAA
                     NTLPVMYSVLLGMTIALFPVLIVLAVVSSLSWAIIKGYVYTIAYLQMWPILFSILNHA
                     MNFYLQGKLSGTAVTLANFDQVQNTYSDIGTTAGWLALSIPFIAYGMVKGLGQVVSQA
                     GNYLGQTLQSASTQSSSQTIDGNWHYNNMTTGNVQGNKWDTNYSHREGHMTSQLESGA
                     MKTLTSSGQSVYNTTEAISKLPVDIALGKTATSSWQQQQRDAMSEAQSLTNSLSQTSS
                     LAASQMSQWSQQRGNSDTTVAGSDISSSSNLTKALNTIQSIGNRYARDENVSLAEAVR
                     EAATKSQEASAGGGLQVSFDTDRHILGKIGGLATGLKGSAEGHVKYAGRSGSSHGTSS
                     DLTRQGGSSKGFSAQELKDFRDAMDVVASSRVTDSGSHTDNESASLASQLSSTFSSLK
                     SQASQYNDAVTRSHEYAQLASYAENNSANINQNYSQEFVGYVTSQRPGEADQLLSDTA
                     SPEVRAERERLVQAFVEERIKPQLLQQFEQNKARTGEGMGSVSSSSGGGDLESEYQVQ
                     QMQMENRANESGVRQQGDVSGDVSDKRRHAESQVHDEKNTVISSGNTQKEEYNRLQSE
                     HEQGNKEFDKAKKEEEYRQSAIPLDAGEAKENIDKLKNEMIKRSDF"
     gene            complement(17054..18421)
                     /gene="traH"
                     /locus_tag="N7B39_001371"
     CDS             complement(17054..18421)
                     /gene="traH"
                     /locus_tag="N7B39_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraH"
                     /protein_id="HGU2421379.1"
                     /translation="MKTFLRQSFLSLLIGTALCTSASAGLQDDMNSFFNNMSYASNAT
                     SAKAWQGQAARYVTGGSFYARTGNKNIQLISISLPSINAGCGGIDVYLGSFSFINSDQ
                     IMAFVKQTMANAAGYFFDLALETTVPELKAAKDFLQKMAADLNRFNMSSCQAAKAMVD
                     SVASLWGESQQNVCQSVAGQNNVFSDWVSSRQGCTSGGKYESVTNKATGAEKDQVLKD
                     INLMWDALSNSTLSSNAELRQFAMSISGSVIFGSNGEMRILSSLASDRSLLSAMMSGG
                     SAKVYVCDNQNKCLSPSLNNVTISESKSLIRMVRDTLTSIENKAITDTPLTEREKQFI
                     NSTSIPILSWIVDQSSLSVSQSLFAQLTDYIAVDIYLQYLEAVMKVVNGSLATKDYPG
                     ANMNELKNGLADARQALNSLRMEVQIKENALISAQQQIRFIRQQLSSKMSDRVLGNYQ
                     FSRVN"
     gene            complement(18418..18951)
                     /locus_tag="N7B39_001372"
     CDS             complement(18418..18951)
                     /locus_tag="N7B39_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2421380.1"
                     /translation="MTAENKFPASADALDRTLRILSEAGCFEGREDIVFFLVGQGYQV
                     ECLNKDSIDIISPMNEKYRLYGTLYSAGNSKIFNILQRRLWGGFWLALLAAFVSRFLW
                     SAVFEAITSPAQSEFWLSLPEDVRTDLNMWGYLIPPVFISVSVTFFVMLTLISVVQYL
                     KVFIFGRFPLYLRKGEQ"
     gene            complement(18963..19550)
                     /gene="trbB"
                     /locus_tag="N7B39_001373"
     CDS             complement(18963..19550)
                     /gene="trbB"
                     /locus_tag="N7B39_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HGU2421381.1"
                     /translation="MLILVLRTAILMAAMLSGCLLAGTLDDIRELEAGKNGRSAPALD
                     GLRLPESNTPPSPGLSEKASPIWYPLSDGRRVNLQDWKLVLFMQSTCQYCRQFAPVLK
                     SLSQQTGLSVFPVSLDGKGDAEFPDVLPATPDVMVEFFQSGVPVATPTTFLTNVNTME
                     TWPMLQGAAEAGEVRKRLDDVLRMALDRQALQKTQ"
     gene            complement(19574..20353)
                     /gene="traF"
                     /locus_tag="N7B39_001374"
     CDS             complement(19574..20353)
                     /gene="traF"
                     /locus_tag="N7B39_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HGU2421382.1"
                     /translation="MRAVAGVLVLLATASLHAAPDDVLTPNYAREEGWQWYNPNAEDT
                     ESGATQVPTLQERLVSMTPSEQKKVLQQATREALDTAILYPSAENFRRFMMLQNFWTD
                     RATDFTQTSKLARLKYPELDYNVKRSHYNGSVEARLTEERKVQNAVIRQVAQRYGLFF
                     FYRGNNAVDNLMAGVIRAFCEDRGITLMAVSVDGKISDQLPQSRPDSGQAEQMRATHF
                     PATFLVDPKTHQWQPLAWGFMSHDDLDRQMVNVLTHFKPDY"
     gene            complement(20350..22188)
                     /gene="traN"
                     /locus_tag="N7B39_001375"
     CDS             complement(20350..22188)
                     /gene="traN"
                     /locus_tag="N7B39_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HGU2421383.1"
                     /translation="MKRYLPLLMLAASFCLVQPLVANEQFNQGMNLSSQNKGQGASAI
                     QGFNPAEVIPGYTDSPPESGYYGGVTSSGVDMTAPGSAALNTSEAGKIITESILNTPP
                     DNKPSLDAPFISEGLAMKDKAETITGGGFAGCVDQPASFTETTTHQCLRDPKIEQYCT
                     RTATITGHYEDTTEIKQIVIESSSIRFTEKNNSFSGEFKVSTGGKVIAASAVYSWNKK
                     LAYGNDHWFMRVTTPFGLISMDDSEGSVTLSSGLNITDGQSLPFSITNRRNKPTVSSI
                     WYSSNMKYRFTITLTIQVPSRKWVPEVVWTESCPFSKSEGAMTGSQCVEPGGTRTVVV
                     DGKTYSIHQDCWKWQDTYLTQTETEGTCGEYMKNPACTVTRSECADQVDGFCVSQQVT
                     YSCERKKEGNGQICGGEFFCKDGSCAQAQTGTSNMFGQAVSALAAVATAGKDVAELNG
                     ENVRAFTGTAKSCKKMAAGFNNCCKDSGWGQDIGLSSCSSDEKALGKAKEKKLTVYVG
                     EYCSKKVLGVCLEKKRGYCVFDSKLARIVQEQGRRGQLGIGFGSGKSPDCRGITVDEL
                     QKLDFGVMNFSDFYDDLNAGSDIPEDQALLKKAQDIIAEKMKENAP"
     gene            complement(22185..22799)
                     /gene="trbC"
                     /locus_tag="N7B39_001376"
     CDS             complement(22185..22799)
                     /gene="trbC"
                     /locus_tag="N7B39_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001485391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HGU2421384.1"
                     /translation="MKRVMVSLALLLVSGGGMANNHTENRQFIHEQLQLDRQRFQSLQ
                     TPEFLKQTRPVAPQEQSFLDAQAQQFRQSMQPGERPVNTALVFVSFSMPAEELKQRVK
                     DAAQLNIPVVIRGMVNGDMRATANAVAGLVKESNTGGVQIDPTTFRKYNITAVPVLVV
                     ACGNQGEKVDRLQGDLTLHQALKRVAEEGDCADTARSLLGEEGE"
     gene            complement(22796..23164)
                     /locus_tag="N7B39_001377"
     CDS             complement(22796..23164)
                     /locus_tag="N7B39_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2421385.1"
                     /translation="MLVSKKKYDFLLARYEQAASRADLLERQQAELNGALQKNGGPFK
                     CILECREAAMALSSSGSEQVWLTLERLAFVDRWISGLLPVLRRQMSDRERRMWEEMLD
                     IRSADRAYGMAHRPSQEETR"
     gene            complement(23175..24161)
                     /gene="traU"
                     /locus_tag="N7B39_001378"
     CDS             complement(23175..24161)
                     /gene="traU"
                     /locus_tag="N7B39_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HGU2421386.1"
                     /translation="MNRYVTGLALMLLCLTGTARAASSECEGNFVNPITDICWECIFP
                     VTIGNVPVAKGRQPDTPNPSMPIQFCPMGILYRVGLAIGYWEPMALTDVTRSPYCMVN
                     LGGFNINIGNVGSGSGGQESRAKPGAFYHVHWYKYPLTYWLNIITSMGCLQGGDLDIG
                     YLSELDPMWDSSSLSMIIQPEAILFGNIIAQGACAADAVASLVSKPIDALFWCAGSQG
                     SMYPFNGYVSNEFSPLQTSLLLSERMAFKLHRQGMIMNSIGENRAVCFEYPSPIIPKS
                     RWRYHMVNKYPDAGQCHPLGRSVTRWEAGKNMPNDRRNFGYLFWRKRNCVFL"
     gene            complement(24158..24793)
                     /gene="traW"
                     /locus_tag="N7B39_001379"
     CDS             complement(24158..24793)
                     /gene="traW"
                     /locus_tag="N7B39_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001485389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HGU2421387.1"
                     /translation="MYRRLLILLALSGMVQAKNLGTWGEMYPIAEQDMLTTIQTRLKA
                     MEASGEMAREQEAFKQRVIENTLRPKPVEGLTLAQENTTHYIDPSLTVSEDLKDHQGR
                     VFARKGQVINPLDTVPFTDTLYFIDADNPQQLAWMKKQNPGTLTYRVILVNGDVREAT
                     NALNTRIYFDQDGSLSRKFELKTIPARVTLAEDRRRLRVDSFALDVPEVKP"
     gene            complement(24793..25257)
                     /gene="trbI"
                     /locus_tag="N7B39_001380"
     CDS             complement(24793..25257)
                     /gene="trbI"
                     /locus_tag="N7B39_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HGU2421388.1"
                     /translation="MTTEQKLNSAPSESSVSAESFRKGGNINVKGSRREQRRRRCLRN
                     LLLVAATVLCVNAAVTSLLISWRTPTVVSFDMKGTVDQFTDQAGAQSLNEAQMSVLTE
                     RFMQTLSTELQEYQRDHNVLILVTPAVVSGAADITGEIQSAVAQKMAAGGGQ"
     gene            complement(25254..27845)
                     /gene="traC"
                     /locus_tag="N7B39_001381"
     CDS             complement(25254..27845)
                     /gene="traC"
                     /locus_tag="N7B39_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HGU2421389.1"
                     /translation="MKKFLSVLENLTNAFRMPDGASEMTRNLASLDFPQLTSVLPYRD
                     YDRDSGLFVNRSTVGFLLRAEPLIGANEQIVYVLEDLIKSKLPRKTPISFHLVSSKLI
                     GEQLDRGLSEFRWQGKRADKFNRITRAFYRRAAQQQFNSPTGLPLTLRDYRLYISYCV
                     KAKKRTAAVMTEVAHTLKVLRSSLDAAKIFTQTADAQELATLVHGMLNARHDQLYPDD
                     VRVGKFDELHQRCIDQRIGIDVRPDDVRISLADRQGSVTTTRAMNFMLENNPDMFMLW
                     MGGDNLSNLLSPDLTIASPFVITMVMEAEDQVATQGEATRKYLDRDKKAKSPYATLFP
                     GVAKQAQEWKDIRERLNSNQTCIVRYYYNITTFCPDTDEDALVCEQQVINTFKKNGIE
                     LFSPTYMQFRNWIAMIPFIHAEGLWEDIKMGGATCRAESIQAVNLLPVVADNRLCQGG
                     LLTPSYRNQLAFLDIYGDGMGNTNYNMAVTGTSGAGKTGLVQPILRSVLDSGGIAWVF
                     DMGDGYKSFCENVGGTYLDGKSLKFNPFANISNINESGERIRDQLAVLASPNGTLDEV
                     HHSLLLRGVQEAWEEHRGTARIDHVVHKLTAIREHDKYQNSPGITNRLDEIIELLGKY
                     CSWGIYGEYFNSDEPSLTDDARFIVLELGGLQDKPDLLVAVMFSLIIYIEDKMYRTPR
                     SLKKCCTIDEGWKLLNFKNEKVGQFIETGYRTVRRHRGAFITISQNIKDFDADDASGA
                     AKAAWGNSAFKVVLKQDASEFKQYNQNRPNQFSELERSVISKFGDAKDQWFSSFMLRI
                     NDTCSFHRLFVDPLSRAMFSSKGDDFEFIRQCREQNMDIHDAVYQLAQRNFPDEMREL
                     EALAEAA"
     gene            complement(27856..28455)
                     /gene="traV"
                     /locus_tag="N7B39_001382"
     CDS             complement(27856..28455)
                     /gene="traV"
                     /locus_tag="N7B39_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001486579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HGU2421390.1"
                     /translation="MKNVTFLLAGSVLLLTGCAGVKSSFDCDATTSDTCMTMTKANQL
                     ARDKAAKQAGKPAAGGLPSLVNLPATSAAVLPSASRSAVSTSSRIQVTSNKPAVSVDG
                     ATGKSTNAAAPTLTPRLVAGTPVTPVSRPAVSVGNPAPSCQTVRCDNPGSVHPQRSRD
                     RIATVWIAPWVDSDNAFHQPGRVSFVVSPADWVLPARIN"
     gene            complement(28543..28923)
                     /locus_tag="N7B39_001383"
     CDS             complement(28543..28923)
                     /locus_tag="N7B39_001383"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2421391.1"
                     /translation="MTTNSNGAPGKIRHLNDIAVCCENPGMRVICVDDAESDFCLSAS
                     LPCATVLEGKSRISQDVLVLQRERGHCLFPGTPGAGMSITKQPMSRVFLAKSDDSQIK
                     ALYSGEDGRGWQRLSVRTVTSGIH"
     gene            complement(28907..29092)
                     /locus_tag="N7B39_001384"
     CDS             complement(28907..29092)
                     /locus_tag="N7B39_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008324139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2421392.1"
                     /translation="MKNRKTNVMGVGKTGSGKTVISVDVMRTFLSEQQLRDWRELRKN
                     IQKDGKSAGDKDHDNQQ"
     gene            complement(29096..29320)
                     /locus_tag="N7B39_001385"
     CDS             complement(29096..29320)
                     /locus_tag="N7B39_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008324141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HGU2421393.1"
                     /translation="MADVLDHLQKQEDLLNRLHIQAARQLIIRGGESLCECECCGNDI
                     PLKRQQTIPGVRTCTECQRVLEIRQKHYLR"
     gene            complement(29432..30796)
                     /gene="traB"
                     /locus_tag="N7B39_001386"
     CDS             complement(29432..30796)
                     /gene="traB"
                     /locus_tag="N7B39_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer pilus assembly protein
                     TraB"
                     /protein_id="HGU2421394.1"
                     /translation="MANVNKIVRRRQLALFIALVLGIGAGGAGTWMVSEMNLKKAPPA
                     KAPKGEPAPDMTGVVNQSFDNKVQRSAIAEAQRLNKETQAEIKKLRTEMGIIGRDLKG
                     SQERIRELEDENKLLQTQIEAGKNFDSLSAEPLPGALASQGKTAAQAGDVPPPTTFWP
                     AGGGQAPVAPVMTPVQRPGMMDSHEFTQPDTAPQKPRFPWISSGSFAEAIVVEGADAN
                     ASVTGDKNTVPMQLRLTGKVQMPNDQEFDLTGCFVTLEAWGDVSSERAIVRTRSISCK
                     LGDDNIDQKIAGHVSFMGKNGIKGEVVMRNGQILLYAGGSGFLDGIGKGIEKASSTTV
                     GVGATASMSAGEIGQAGLGGGVSSAAKTLSDYYIKRAEQYHPVIPIGAGNEVTLVFQD
                     GFQLETLEEARAKAAARRKQNQPSASSAPAAMPGNTTDMLKHLQDFRLGDTVDPATGQ
                     VVTQ"
     gene            complement(30783..31523)
                     /gene="traK"
                     /locus_tag="N7B39_001387"
     CDS             complement(30783..31523)
                     /gene="traK"
                     /locus_tag="N7B39_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008455355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HGU2421395.1"
                     /translation="MKNNFPALLLGTAMMVTSSGTVYAQSPATLSFPQGGQFRLSISN
                     TDPNMIFIPGDKVTAITAQNGMLADKRLTSAGGVLFTSVATRAFTLYVETALGQTFSV
                     VATPVKGEGRVYRLMSAEPPSRPETRQWETAQAYEKLLIALTRSVLTDKMPDGYGEVK
                     PLADGINVPGGFSATPKKAWAGDQLRVDRYELRNANTWGLALREQDFWKPGVRAVMFD
                     SNAQTLMGGGRLTVTVIRENGEGTDGQR"
     gene            complement(31513..32076)
                     /gene="traE"
                     /locus_tag="N7B39_001388"
     CDS             complement(31513..32076)
                     /gene="traE"
                     /locus_tag="N7B39_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HGU2421396.1"
                     /translation="MEISARNSSTKIIAIIILSLTALVFILGFAVTWLAVTNRTLIHE
                     QRTVVTPMTYNAPFAVSETKADTEFFRMMTLSFLALRLNVSPETVDSNHAFLMSFVEP
                     EVREEFKKVLQEEAAQIKASDVNSTFYTTEIKVYPVDGRVDVRGVLKMWIGNSKPTTE
                     LKNYRLRLKYAGGFTRIGRFYEVTNEK"
     gene            complement(32096..32401)
                     /gene="traL"
                     /locus_tag="N7B39_001389"
     CDS             complement(32096..32401)
                     /gene="traL"
                     /locus_tag="N7B39_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032490353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HGU2421397.1"
                     /translation="MSGNDLEKFRFPKTLSEQNRIIGLPLDEAIPAAVLVLWGFFAKK
                     YVFGLIIATVVWQLIRAAKRGKSSRWLYNWCYWHLPVELFRVVYRVIPDSSYRKWIK"
     gene            complement(32403..32762)
                     /gene="traA"
                     /locus_tag="N7B39_001390"
     CDS             complement(32403..32762)
                     /gene="traA"
                     /locus_tag="N7B39_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001404412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HGU2421398.1"
                     /translation="MNLSLSKGGRPASVKNRAWHYCQMAWRGVCNQKALSRLAAFSPL
                     LLLGMGQMAHATDLLAGGKDDVKATFGADSFVIMCIYIAEIVIGVATYIRTKNLLILL
                     GLVVVIVFTTVGLTFVS"
     gene            complement(33271..33546)
                     /locus_tag="N7B39_001391"
     CDS             complement(33271..33546)
                     /locus_tag="N7B39_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraJ"
                     /protein_id="HGU2421399.1"
                     /translation="MVIINPIFMSKLSKIFPEISDQKIEILTFFVYGMSTKDIAYYKG
                     VSVQAISKNLRELCEVYAVPTTDMLKTVYTVRLDLYNHLRFSYEEHS"
BASE COUNT         7629 a         9691 c         8369 g         8010 t
ORIGIN      
        1 agatacattg caccctgtaa gactggcggc tggcgcttta tgacacgaac ggttgtaacc
       61 ttatagtaaa gtcccttgca gttaaatgtg gataagcaaa attacctgtc tgtgaggcgt
      121 gttttgtatc aaaaacaggg gggaccggat gcaccagaag gtggatgatg agattgtttt
      181 ttgtatgtgg tgctgatttt ttgtgcgctg tcgggcttca gacgtgcgga agcctccggc
      241 gcaggccgga ttattctgag gaggtcactt tcagggggaa gcggtggcca gccggctgta
      301 attgcggtta cgtgacagaa tcatgcgctc cttcacacga cgctccactt cacgttttac
      361 cgcctcacga ctggcagtga agcgcccttc cgcgatttca cgcgtcagct gccgtttcac
      421 cagggtgacg atatcctgac gttccctgcc cgcatcacga cgcgcacggg cacgctttat
      481 tccccgggac ttaagctctg tctggtaact gcggaaacgc tcacggacaa aacgccaggc
      541 tttcgctatc agctcatcca tacccagggt atccagcccc tgctttttgc gctgtctgtt
      601 ttcccattca acacgactgc ggcgcgcagc tgccactgca tcctcagaca catcgagagc
      661 ggcaaacagc gccagtgtga acgtgatgtc ggtcggaatg tagcacccga taagcgggtc
      721 atattccgtc tggtaggtaa tcaaccccag ctctgacagg aacgtcaggg cacgggtggc
      781 acgagtgatg gagagttttc ctgcaccgga ctctgtcgcc agtccgcact caatggccag
      841 cgtggtgatg gagcactgga cgcggttggc cagcggatca tagtgaaaac agagtccctg
      901 cagcagcgca tcaatggccc ggcgacgcag caccggtggc atgcgccgac gcagaccacg
      961 cgaacgggca tgcgccacat gaatggcgaa atcaaaacgg gaggtgaagc ccaccgcctt
     1021 ttccatcagt ttttcgcaga acttcagcgt tccggcacct ttacggggtg taaacaccgg
     1081 attcgggttc tttacctggc ggtaatacgt ttgttgaaga tcagtcacac catcctgcac
     1141 ttacaatgcg cagaaggagt gagcacagaa agaagtcttg aacttttccg ggcatataac
     1201 tatactcccc gcatagctga attgttggct atacggtttt tagatggccc cggtaatctt
     1261 tctcactcgc caaagttaaa gaagattatc ggggttttca cttttctggc tcctgtaaat
     1321 ccacatcaga accagttccc tgccacctta tggcgtggcc agccataaaa ttccttaaac
     1381 gatcagtaat ctagcatgct acgccacaaa gtaaagtctt tactttagta tatccagtct
     1441 ctgcagttca tctttgatga ttttctcaac gaactgagcc tgtgttatcc cctctctctc
     1501 gcagtactca accatgagat ctttcagagg atttttgaca aaaactttta tctctttgtg
     1561 tgtaagacgt tttcttgcaa cagcggccat ttgtttctca gagtcagtca taggcttacc
     1621 tctgcgcaca aaccgctttt gactcaatga ggaagtcact gcattttctg tctgcgacat
     1681 ctcgcctcct caattctcaa acagggatcg tttcgcagag gatactacag ttttttgaaa
     1741 tcagcgactt gaaagttgtg acgatgatct gtgttggcgc acaaatcatg tctgattacg
     1801 tccgtttaat gtgattttga taatgaaata acgaaaaaac agaaaatacc taaacctgtg
     1861 tactggcccg ggcgggatcg gcggtttctg gtatataggg tgtctgcccc tgaaatactt
     1921 cacactgccg ggcttcatag gccagagtta cctgcaccac tgtatttccc ctgtgaatat
     1981 tcagctcaca taaccgttcc ctgaatatca gggaaaaaca caacaccgtg gcgcacacgg
     2041 cgaacaaccc tataaaggca tattttcccg tttccggtgt tggataactc caccatgcgg
     2101 aggcgtcagc cagtcgagcg acgtcaaaat tcccggatca acgactctat ctctacagga
     2161 accttacgat tattcaccaa acgcacatca agcggatgat tactgaccag gttaagagca
     2221 cgcctaagag cagagacaat ccagctgaga tcattaccga aagcgcctcc accaaccaga
     2281 gtcagataga ctctgggatt acctgtgtcg tgataattta gcaccgcagc cagcagggtt
     2341 gcttcataac aggcctcaag cactaaagtc gcaaaaggac cccagtccct tcgtggggaa
     2401 tagtgataag caaccggtaa agcagcgcaa agaacctgag taacgcgctg ctccttcggt
     2461 acacccggaa ttgtcacttc cgtgtcagaa tgaaccccga cacggagctt ctgcctcacc
     2521 tcatcacgcg aaagttcatc catcgcctca atatgtttgc tgatggcgag caccgtatcc
     2581 ggttgcagaa gagcgtagcc attttgcatt ctgatccccc taatgccaat ggctctgtcc
     2641 atgtccgcca gagcgttgag ttgacagttt tgagtctgcc ccctctgcga accaaccggg
     2701 accagatagt tacgaaacac cgtagcggca gcacaggcca ttgcacatgc gggcccctgc
     2761 gtataatcat actgatagtt ggtgattccc ttctccggtg tagtgtgttc gttcggcatt
     2821 tccaggaggt taaactgact ggcaacctgg attattgcgc gattcgcctc cggacaccga
     2881 tgaagttcat aagcgtcggt caccaaacta cttagcttta ttttttcacc tctacttacc
     2941 tccaggctac aaacggcttt ccgtatttca gccagactcg gcagagtcag tataccggca
     3001 tgaaaggtac gggatgactg gcccctggta tgaagcaagg gaccatcgat gtagaaatta
     3061 gcctgagttc tggcgtaagt tgattcctca aaaccatata tatcgctaaa ccaactcatt
     3121 ggtttactcc tttctggaag ctaaatttat tcagaaacgc cggaagcggg gaaaacttgc
     3181 tttttcccgt tttcggggtt ggacaactga gcagcgcgaa ggcgtcagcc cacctgacgt
     3241 gtatgttctt tgattttcgc cggacatcca ctacctcctg aagaacgtgg tggatgtccg
     3301 gccttacccc tgctgcgccg ttggcagtat tctgctctct gccacgcaaa ctgctgccag
     3361 tcagtacggg gagcaatatg agcagccagc caccatacat ccgcagcctc tgcccagagt
     3421 tccctgacct ccagccggac ggctcggttt ccgacacacc gataacgcca gacctgactg
     3481 cttcgtgaac ggtttctgaa tccattcatt cctctctcct ctttaattcc gcctgctaaa
     3541 tatgttaaaa aataaccgta atccgacttc caacgggcat gggagttgtt tcatcagacc
     3601 agccttattc ttccgcctcc cttcatctct gagggaggca tttttttgct gttcagtaat
     3661 actaaagcac cagaccactg tatcggagtc aggaacggtc agagaactct gaccagaaag
     3721 ccaataaaaa acagatgaat ggcatacgct ggcgcaaacc agcgtcctgc ctgcagacgt
     3781 cgtccggaag ccggtaccag cccgtggaca cagaacagca ccgtcagtgt cagaatcacg
     3841 ccggaaaaca tcatcacatc accgtgtcgg gcattaagca gggcaaccag caacagccag
     3901 acagcaaaca gagctggacg cagcgtgccc ctgacctgcc agaccagcag cgctccggca
     3961 agcatcagca gaccggggat gccgtagctg gcagaactga acggtgtata ccccatcagt
     4021 accgtgaagg ccgcaacacc attacgggca gtagcagact gcaggaaaag agccacctgc
     4081 atgacaacag caaaggtgaa caggatattc agttgccacc acgcaaggcc gacatcccgg
     4141 aaggccagcc agtaacccgg ctgtgccagg agtgccatca gccacaggcg gttcaggctg
     4201 tgctgacgta aggttttacc cgccatgttg cagccacaga ccagcgcaaa cagggggaaa
     4261 gcacagcgcc cgcccatgtt cagtaagggc atatccagct gaaaggcagt agctatgtgg
     4321 tcccccacca tcgccacaaa cgccaccatc cggatgacat cgcgctgcag cgcggagaaa
     4381 ttcaggaccg gcctgacagg ttgcacagca acggatttac cctgattcat gctgcggcct
     4441 ccggacgctg tgcgcaaagg tgcgccctga cacgcgccgc cagttgctgt tcattatcgc
     4501 cacgcaacag cgggataccc gcctgtttca gcacctcttc cagcagcagg tcacgccgct
     4561 gacgtttcgg ttcctgatga gaccggtcgt ccagctcaac cgctgcaatg atgcgcccgg
     4621 cacggtccgt gataaccaca tcgcagtgcc actgtgacac caggcgaaat aactgccacc
     4681 attcacggct gccggactta cggtcgggcg ccactctgac aatatcggcc acccgaacct
     4741 gtggacacag acgccagtgg cgggtatcag tcaccctgtc cagccggcgc agaaagcgct
     4801 gctcccgttc ggtcatcaac cggccggcac tgacaaatga tggcctgctg cacagcaaca
     4861 gctctgctgc gccaggccgt ataccccttg accgcaatga ctgcagaagt ggatgcgctg
     4921 cacgaccggc ctgacggagc aggagcacaa acccgcccag aagaatgaca ataataataa
     4981 atgcaataaa catacgttcc tctccctgat tcagtcgcca ccgagcgtct tttccagctc
     5041 tatctctttt tccggcatct tcaccaccca ctcacgttca gtacgctgca tctgttcacg
     5101 tacctgctgg cgggcaccac gaccaccggc aaccgcttcc cggacagctt catccagttc
     5161 ccggctttcc cgttctgtac cgtcacgcag gcggcgctca tcatcggcag ataacaacgg
     5221 gtcacgtttg tcttcgtctt tttgtggtgc caccgacaac agttcacgcc gggcctgttc
     5281 cgccagttca cgggcggctt tctcctgcac ctccgccagc ttctgctgct catccggagg
     5341 cagcgtaatg gggtcttccg cttttgccgc cgtttcggcg tccgccacgt tacgcaatgc
     5401 ccgactcaac tcgtcaggct ctgtgatacg tcccccggtc agccgcgcgg cattgagcag
     5461 gcgctcttcg ccgtgcaggc gtatcaccac cccactctcc gggttttcct gcgccagccg
     5521 cagtgcggcg tccgcactgt cagctatcag tgtctgacca ttccggctgg cctgcagtcc
     5581 ggcaaactgc gcatcttctc cgccacgcag ccgggattct tcgctgagca ccacgcgcat
     5641 tccgtcatcc tcaatacgaa tttccgtcag cagcgcccct gcctctttcc cgtgacgggt
     5701 ccagactggc agggccacat acggtgacgg gtacttttta ccggcagcaa tgaagcgggc
     5761 catcgtctca ccctccagac cgttttccgc cagcacccgg cggcccagcg ccgttttatc
     5821 cagccgcgag gcggtggcca gcaggcggtt accggtgtcg gactcgcggt cacttttctc
     5881 gtgcagcagg tcgtgggcgg ttttgccgcc gtcagactgc cggacctgtt gctgccagtc
     5941 ctcaaggttg tccgtgtaga cctggacatg ctcttttgcg cgtgacaacg tcacataggt
     6001 ggattccagg gacgccattc tcttcctgcc gccttcggtg ccggtcaggg caatagcaaa
     6061 acgctcgctg gctccctgta cgccatagac tgtcagggcg taggcgaggt caatatgacg
     6121 gtcctccgtc atggcctgcg gatcgacaat tttctcctga tctccctggc gaaagcggat
     6181 ggccccttcg tcagtgaacc cggccacctc ccacaggctg ttggccacgt agccgcggtc
     6241 ggtgtccgag cgggtaaagc gcacacggtc cccctgactt atcgtcagat cgcggggtgt
     6301 gaagagggaa atatcctgtg cagtgttctg ctgaggagag agcagcactg actccccgtc
     6361 agcattacgc agcgtgacca cggcgtcctg cgtatccacc tctgccacag tccagtagcg
     6421 ctcgttcatc atggccacgg cgccggtgta ctccgcaaag gtttcagccg cacgcagggc
     6481 gttgtctggc acccggagcg gctccagcac ggtgaacgta cgttcttctg cgccggtatc
     6541 tcccttctca tgccgggcga catgaatggc ctcattgatg gcatgccggt cagcgttgag
     6601 ctctgccata atcagcgtct gctgctgtgc ctccggggta cgccccatcc agtcatcccg
     6661 gatggctgca atgatgtcgg tacgcgccgg tgacaactgc tccggcttca aagcctcctg
     6721 tgctgcagtc cggccctgct ccggctcttt ctccggctta cggatttcca taacggactt
     6781 ttccggaacc catgccccgg cctgacgcgg gacctgctgt gggctgacgt catccacctg
     6841 gcgcagggat gtctccacct gacctgcgat gatgctttca atcgccggac gcaacgccgg
     6901 ggtctgacgg acaatctcct gcatcaccac cgtatcgatg gcactgcgct gttgtaccag
     6961 acggaacggc tgaccagtgg aaacagcctg caactgcgcg gtgtccccgc tggagaccat
     7021 ccgcccgccc cctgcagcaa ccagctgata aagctcagcc atatcgcggt tacccaccat
     7081 cgagctttca tcagtcagga acaggacgtt acgaaaatcc ggcgtttccc cggcctgaat
     7141 ggcctggcgg gtttcgctca gaaaactggc cagtgtgcgg gcatccacgc ctgcttcacg
     7201 catctcatgc acggcccggt gcgtgggtcc cagaccgata acctgcgggc gcacggattc
     7261 agacagcgtg ttcagggccc ccatcaccgc ccggaactgg gttgttttcc ccaccccggc
     7321 gtagccctga atggccataa agcggtcggt attctccagt accgttcgcg tggcttcccg
     7381 ctggcctgca gtcagacctg acagtaccga agcaggcgtc agcgacatca ggggctgtac
     7441 ggcatcctta ccctcagcaa tatggcggat gatgctcttt tccatctcat acgccacccg
     7501 cggaacaaga atcctgtttc ccgctccggc aaccgaatcc agctgaatca gttctccgct
     7561 gctcacctgc cgggcgattt cagtccggac atcagccagc ggctggccag attttaccgc
     7621 ctctgacagc agggtaattt cactgaggtg tacctgtccc tgctgtgcgc gcgggatggc
     7681 cagactgaca gcctgctcag catccgacat cagacgctca cgacttgcct gtatggcgat
     7741 atccaggttt tcctcgccgg tcgactggcg gagctggtca ctggcaagcc ggacggcaga
     7801 cacgctctcc actttacgcg cagcctgcct tgcctccagt ggcgtataaa tccggatatc
     7861 accaccgctc tgcgccagcg cgttcagaac ctgtttattc agtgctcgtg gcccaaccgc
     7921 ggccagtacc gtgcgtgatg cactggtacc cgccccggtt ccctccacat aatcatgggc
     7981 cagcttcacg gcccgctcag tgggcaggcg caactcccgc tcaccactgc gcaatcgtat
     8041 cgcaccattt tccagagcag tcaccgtgaa ctgctccttc gctttgatgg cccccttcag
     8101 ctcacgaccc agcgccctga cgcgatcccc ctctgaaact gccagcgtcc ggctgcggta
     8161 caggctccag ctgccgtcaa gctgcgagac cttcagcgtc aggcgactgc cgtcttctcc
     8221 cagcagaacc aggctgttgg tggcctcagc cacacggtca atggtgtgac gtgtcatcat
     8281 tttcttctca gcgtcccact gctccatcac catcccggcg cggtaattat cgcgctgatg
     8341 acgagttttg ctgtccagcc acacaggctc cagcacccgg attgtcatct gctccctgcc
     8401 cagctccccg gcttcacgac gtgcgtcgcg gatgacatcc gtcagttgct gctgctcccg
     8461 ggcaccggtc acctgggcca ccacatcccg cccttctgac gacaggcgca catagtcctt
     8521 tgccagctgg ccataacggc ttcgcttgtc cgcctcgctg accagtcgca cttccggcag
     8581 ttgagtgcca taaaaccggt actgcgggat accggcttct ttcagcacag acagggcatt
     8641 ccccgttccc tgccggttct ctgtatccat gaatattaat tgcgccccgg cactgagcgc
     8701 cttttcctgc agcagcaccg tctctttcag cgacagccgc tccgcacggt caacgatgac
     8761 cgttccctgc accggcaata cggtgtcagc gttcatctgc gagcggagca tcacatggcc
     8821 tgccagatgc ggactttctg acataaactg gccactgcga ccatcggcag caataaccat
     8881 cacctcacgg ccctgtgaac gggccatcat ggccacgtct tccgtgtgct cacgcagggc
     8941 ctgcgcgcct ccgcgaccac tgagaatggc caccgggctt ttgtcctgcg tcagaacaga
     9001 gacagcatca cctgccggta tatcgcgttc ctgtgcccgt tcaggaaaga gcagcacggt
     9061 ctgttcctga atggccgtgc gcgccagctg atggacgctg agttcattca gcaggtggat
     9121 atcagaggtg aatatcccct tctcacggtc cagagggata agacgctgcc cttcaatcgc
     9181 ggcatcgatg ccggcccggg cctgctcaaa cagaccgggt gcggaaggca gacggttgac
     9241 cgtaccggcc agcatctccg accaggtgaa ctggaccttt ttctccgaca acgccgaaat
     9301 cgtgtcgctg acagctttcc gcacctgctc gtcagcaatc tcgccgaaag acgcgcctcc
     9361 cgacaggtcc ggtgcatgta taccgctatg tctacctgca acagcaccag gaacctccac
     9421 gcgcgcctgc gcctgtctga tgtagccatc gatatcaaat ttttcatcgg ccagccgccg
     9481 gcgccagtca gctttcagca gctccgggtc ggcccaggct ttggcctgac gggtatcgag
     9541 cgcggcaacg tcgcgggatt tggcggacgc gtccgggccg gcggcctcca ggatagtctg
     9601 actgcgtgag gaaaaaacat cgacagggac atccttcagc tcccacagac cattctttcc
     9661 ggccgcgaca gtttcaaatc ccatcgactc aacgtcttca cgtaaagcag aacgatacag
     9721 gtttcccagc gctatcttgg tggcatacag attctcggag aaccccgttt tcatgcgggt
     9781 atcactggcc agcgaccgcc atttttcatc agcaaacgtg gcgttaaaga ccagagcgtg
     9841 ggtatgcaac tgcgggtcga ggtcacggga cgtatcatgg ttatagagtg cggccaccat
     9901 gttgctggta agtactgttt ccgttttccc ctcttctgta atgcgcgcgc tgaccagctt
     9961 ctcaacctcc tgcatcacca ccgctacagc ccggttatgc gcctcaataa agcgtcggtc
    10021 ttccccgacc agcgccatga cggagacact tttgggggca gaaaacgtca ggtcgtagcc
    10081 gctgcggtgc ttattcacac catttaccat acgggagaga tctgaaccgt ccggaagttt
    10141 accctgacgc acagcatcca tatcggcgct ggcgacttcc cccttcagtc ccagtttctc
    10201 cgcaccctcc cccatccagc gggagtccag tgaaccgctg gcgtagtagt tgtcttcatg
    10261 actgtagtag cccgcatcac ctttaatgga agaaatggag agcatcagaa cacctccctg
    10321 tcgtcatcaa aatcacgcgc tttatctgtc gcatgattga tgttgacctc ttccctgcgg
    10381 gtcatatcgc gggtctgatg tgaagcctga acccagtcct catacgcttc ggaatccacc
    10441 acctcaccat cgctgttcag gcccggagga acgtccttag gtaaatcctg ttcaatatcc
    10501 cgctcagttc ctccggtgga ggtggagcca ccttccgctg caacagcagt ggatcccgtg
    10561 tttttggtat ctgcatcgcc ggcagcgttt tctgtctgta atgtgcgcgt ctcaggaggt
    10621 gtgttcgttt ccgcaagaac agcagccacc cggccagcag aaggaggggg cgtattcgac
    10681 agattactct ccggaacagc ctgaattaac gacgcccctt cgaacaggcc ggcaaactta
    10741 tgcctctctt cttcagtgcg acgggtaatt tcatcttcaa gcatctgatc cagactggtc
    10801 tgtacatcac gcaacaacag cgagtcagcc actttgggca tcgcctcata cttcattgtc
    10861 agtttcacca ccggataact tcccggcagg gtgacaaaac actgcaggtc gttcagggac
    10921 tgcacatcgg agtagctgac aatactgacg cgttcttctt ccttgccaaa agaaatcccg
    10981 tcacgcacct gttcatgacc aaagctggtc tgttcactga atttttttcg ccaggtttca
    11041 ccgatatctt cctccacaaa cttcgcaatc tgggcgctcg gggagcggaa gaagaatttg
    11101 gtattcagca ggtcaaacag cgccgccgcc gctttcgggc cgtaaatttc ttccagctgt
    11161 gcgaagctct ggaagcccag gacaaaacag ccgccaaact tacgcgcctc cgaaataatt
    11221 cgtggcagag tcggtaattt gtgcaaagaa gccagttcgt cataaaaaaa ccagacacgt
    11281 cgctggcggt tcggccccat cgccagcaga ctggtggctg caatacttag ccacagtgaa
    11341 atcaatggct taagagactc atggttctgt tcgtcggagg taataaagag ccagccattt
    11401 ttcgatttat cggcctggcc cttcatccat tcccggatgg taaacttttc gcgtccgggc
    11461 ctgtcgatac cctgcagata acgcatcgcc ttgacgtagt tagtcagcac gctgcggatg
    11521 gaaatggcgg tcttctcaat cttaccgtca actagggtgg aagcgggcgt gccggcaaga
    11581 aacgttctca gttgatcaag ctgaatagcc agcagagtgc gcaggaattt attgtagctg
    11641 cggtcttcat ccctgcgcat gcgttctgcg ccttccgcaa agatggtacg cgcactgccc
    11701 tgccagaacg gatcttcacc actgctggcc gggatcaagg ttgcactggc attctccagt
    11761 tcggaaatcg tacggcactc ttcccacaaa tcccagttgg ggcaacgggc atccagcgca
    11821 ttaagcactt tatcccggga ttcatcgtag aaatcctcaa tgaacgtaca gcccttgtcg
    11881 tagataatga ccaggtcccc cctgtcgcgc agttgcttca taatcttgcg catcagagtg
    11941 gacttaccgg tcgatacagt accgtgcatg gacatattct gtatctcaga atccagcttc
    12001 agcggcaggt catcgatgcg gatatcggat gcttccccac gttttttcat catccgggca
    12061 acgtcttttg gtgcctcact gagagcgcgg cccccgatga tttcatcctc actctgctgg
    12121 cgaccggtgc gcccgatata ccagtacacg gcgaatgtgg ccacaaaggc gacaagcagc
    12181 gcggcgcaac cagcaataat cagctcctgc ttcagcaact gaccgcaata tacggtgtaa
    12241 tcatcattca gtatctgacg tgcactgtac tgcagcttat gttcgtaata acggatggta
    12301 tagacaggct gactcaccat tttttccgta aaaggtgaaa tcatccggac aaaccagtaa
    12361 ataataccgt tggtcagatt ctggatgctt attcgcatca gcagaacggc aataccaaat
    12421 aacaccgtgc ccatgataac gtaataagaa atcagattat ttatttgcag gaacattttc
    12481 aggcgcataa aagcgacctg accgccctgc gttaaatcgc gaggattcag gctcataata
    12541 ttttccggag aataatactt agtgaagata cttcagaaag aaaccttcac gaacagaacg
    12601 cgttaattct gattgcgtca gagaataaaa taacagggaa acgttttaat acgggtgacc
    12661 gctggccacc cggaaagtct tacaggatat tagcaactga tttcgccagt tgatcttcaa
    12721 gaacaggttt agcttcttca aacttgagat taactttatt agcggaagag acaatacgcg
    12781 tctggtattt atgctgatta cctgtctgcg tactggtctg aaccttatat ccggatgttc
    12841 cttgtttcag tgcagctaca ttatcggtct ggacagtggc attcgttttt tcagaaatct
    12901 gaatgtccgt gaccatggtg tagttaatat cttcaaccat cgcatcagcg gccattccca
    12961 cgagaccggc agcaaggccc acacctaatg tcgcccctgc agagttggag ttataggccg
    13021 taatacctgc ccctaacgcc gcccccattg cagccccctg atagccctgg ctcaggaatc
    13081 cctgagccgt acgcagatcc attttatcgg ctttgaggac attggcctgt atccagtaat
    13141 atgcgctttc cggcgcggat gtcacagtat accctttatc ctgaagcgcc ttcgtcactt
    13201 tcgcctgcag accggacatg tctttgtcgg aggtattctt tatctgaaga tatacggttt
    13261 tctgcgagga cggttcaagc cagatggttt cactcatctg ggttttcact tcaagattac
    13321 gtttcttgat tgctgtgctc atcgcgccac aaccagataa caccagagtg gaagacaata
    13381 tggcagtcat tgccattaac ttaatgtttt gtttcatatt ttctcatttc cgtataagta
    13441 gcatgatatc tttcatgctc agaagtgctg ctatcattcc tcccttccca gagggaggta
    13501 atggctatct tataatcaga aatatactcg catcaatacc acggcctgag cgggttaatc
    13561 gtattgaaat gaccaataaa tgacttaatt ccgagattca gaaagcagtt ctttcatttt
    13621 caaaagacta ctggttactg caggagtaac gtagggtgcc atcatcatat tataaataga
    13681 tgtggaaatt atgaccgtca caaaccaact cccccccata aaagcgatca ttgcagattc
    13741 atttagtgca aaacatatcc caaccaacaa attagttact attgcaaaat ataagacata
    13801 ttgaagttta gccttggcaa tgtttagtaa tactgacttc cgccttgctt catcactcag
    13861 gcacgaaatc atcattatgg gattatggta aacgataata agtataaaag atggaagaag
    13921 caaaacccct aaaaaaccga taaaataaac gatgatcgct tccctacaac ctcccctata
    13981 ctcctccccc caaatataaa agtctagagc acttagaaaa aactgtacta ccagagcgac
    14041 ggctatgatc ttaaataaaa acttaataga gctcgaactg gaagggatct cataatttct
    14101 tttaagatca tcaaaaagca tttctacatc tttttttaaa tcatcagttg ttactttcat
    14161 ctctccctct taaaaatcac tgcgttttat catctcattt ttcaacttat ctatattttc
    14221 ttttgcctct ccagcatcta aaggtattgc tgactgcctg tattcctctt cttttttagc
    14281 tttatcgaat tctttattac cctgttcatg ctcactctgc aagcggttgt attcttcctt
    14341 ctgtgtattg ccggatgaga tcacagtatt tttttcatca tggacctgcg attcagcgtg
    14401 acgacgtttg tcagacacat caccactgac atcgccctgt tgtctcacgc cgctttcgtt
    14461 ggccctgttt tccatttgca tttgctgcac ctgatattca ctttccagat caccgccacc
    14521 agaggaggaa gacacactcc ccatcccctc tccggtacga gctttattct gctcaaattg
    14581 ctgtaacaac tgcggcttta tgcgctcttc cacaaaagcc tgcaccagtc tttcgcgttc
    14641 tgcccgcact tccgggctgg cagtgtcaga aagcagctgg tcagcctctc ccggacgctg
    14701 gctggtgaca tacccgacaa actcctgcga gtagttctgg ttaatgttcg cactgttgtt
    14761 ctctgcatag ctggccagct gggcatactc atggctacgg gtaacggcat cattatattg
    14821 tgacgcctga ctcttcaggc tggagaaggt ggacgacagc tggctggcca gggaagctga
    14881 ctcattgtct gtatgactac cactgtcagt gacacggctg gaggccacca catccattgc
    14941 atcgcgaaaa tctttaagct cctgagcact gaacccttta ctgctcccgc cctggcgggt
    15001 cagatcggaa ctggtcccgt gcgaagatcc acttcggcca gcgtatttga catgcccttc
    15061 agcagagcct ttaagtcctg ttgccagccc accaatttta ccgagtatat ggcgatctgt
    15121 atcaaatgaa acctgaaggc ctccacctgc gctcgcctcc tgactttttg tcgcggcttc
    15181 tctcactgct tcagccagac tgacattttc atcgcgggca tagcggttac caatcgactg
    15241 aatggtattc agcgctttgg tcagattact gctgctcgag atgtcactac ccgccacggt
    15301 ggtgtcgctg ttgccccgct gctgactcca ctggctcatc tgcgacgcgg ccaggctgga
    15361 tgtctgcgac aggctgttag tgaggctttg cgcttcgctc atggcatcac gctgttgctg
    15421 ctgccagctg ctcgttgcgg ttttacccag cgcaatatcg accggcagtt ttgaaatcgc
    15481 ttctgtggtg ttgtaaaccg actgtccgga gctcgtcagc gtcttcatcg ccccgctctc
    15541 cagctgactg gtcatatggc cttcacgatg gctgtagttg gtgtcccact tgttaccctg
    15601 tacgttcccg gttgtcatat tgttgtagtg ccagttaccg tcaatggtct gagacgacga
    15661 ttgcgtggag gcagactgca gggtctgccc cagatagttc cccgcctgcg ataccacctg
    15721 ccccagccct tttaccatcc cgtaagcgat gaacgggatg gacagcgcca gccagccggc
    15781 ggttgtgcca atatccgagt aggtattctg tacctggtca aaattagcca gcgttactgc
    15841 cgtaccactg agcttaccct gcagatagaa gttcatggca tggttcagga tggagaacag
    15901 gatgggccac atctgcagat aggcgatagt atagacatag cctttaataa tggcccagga
    15961 cagggaactc accaccgcaa gcacaatcag tacagggaaa agtgctattg tcatccccag
    16021 cagtacgctg tacatcaccg gcagtgtgtt agccgcaatc gacgcactgg tcgcctgcga
    16081 cagccgcatt tttgccattg aggtctgcgt ggccacattc accagtcccg ccgtatcccc
    16141 gctcagtgcg gaaaaactct ccagtccgct gcgcaggccg ttcattatca cattctttcg
    16201 aatgatctcc gtggatgtca gggaagtacg atgaaaatag ttatagctgt cacccagcat
    16261 ttcagtaaac agcgccgatt gcgccccgtt cgtctgacca aatacctggt cagtgaagcc
    16321 gtgcagcgta ttcaggatct gttgaagaaa aggtgacgta ttcgccccgg aaatatcacc
    16381 ggagtccgta ttaattctgg tggcagcctg ttcacaggtc aggaaattac gatttttgtc
    16441 gtagagccca cgcagcggac tcggacggct gaatatcagt gcataggggt cggccgcatt
    16501 catcagctcc ccgatggtgt acttgttgtt cagcatgatg tcacccacca cgcagttatg
    16561 aacataatcc gagaacatgc gctgaatctc cggctcagag aaccggaaat cactgctccc
    16621 tgcagccagc tgcgcaccaa aaagcatccc ggttttactg taggtcagcg cgtcagggcg
    16681 ggcaaaaatg gtatcgtacg tctgtgccat accggcaccg atccgggtaa tcatactggc
    16741 gggcgcagcc aggcctgcag gaacattatc cacctgataa atccgggccg gttcagacag
    16801 gtcaataatc tgaacgcttc gtttatcgcc aatcaaaacg gcaatgacca gaacataaaa
    16861 cccggcccac ttgaccagat ccataatatt acgggtccgg atataagttg cagcgccgac
    16921 aatgactgac acggcggtgg ccatcgcaat cagcgcatcg cccgcacggc tgtcgagaaa
    16981 cgatgccacg ccattgagcg tatcctgaaa ccacgcacca ccggcaatcg tgtaaatggt
    17041 atcaatggcc atattaattc actctgctga actggtagtt acccagcacg cgatcgctca
    17101 tctttgagga gagctgctgg cggataaagc ggatttgctg ttgtgcagaa ataagcgcat
    17161 tttccttaat ctgaacctcc atacgcagtg agttgagcgc ctggcgcgca tctgccaggc
    17221 catttttcag ttcattcata ttggcccccg gatagtcttt ggtagccagt gaaccattga
    17281 ccaccttcat gacagcttcc agatactgca gataaatatc gacggcgatg taatccgtca
    17341 gctgagcaaa cagggactgc gaaacactca gggatgactg atccactatc caggacagga
    17401 tgggaatgga ggtgctgttg atgaactgct tctctctctc cgtcaatggt gtgtccgtaa
    17461 tggctttatt ttctatgctg gtcaacgtgt cccgcaccat gcggatcaga gattttgact
    17521 ccgaaatggt cacgttattc agggagggtg acagacattt gttctggtta tcacagacgt
    17581 acactttggc gctgccacca ctcatcatcg cactcaacag gctgcggtct gatgccagcg
    17641 aagacaggat tcgcatttcc ccgttactgc cgaaaatcac cgaaccgctg atgctcatgg
    17701 caaactggcg taactctgca ttgctgctga gcgtactgtt gctgagagca tcccacatca
    17761 ggttgatatc cttcaggacc tgatcttttt ctgcgccggt agccttgttc gtgacgcttt
    17821 cgtatttccc gccggatgtg cagccctgac gggaggagac ccagtccgag aacacgttat
    17881 tctgaccggc gacagactgg cagacgttct gctgactttc cccccacagc gacgccacgc
    17941 tgtcgaccat cgcttttgcc gcctgacagc tggacatatt gaaacggtta aggtcagcag
    18001 ccatcttctg caggaagtct tttgccgctt tcaactcagg cacggtagtt tccagtgcaa
    18061 ggtcgaaaaa gtaccccgcc gcgttagcca tcgtctgttt cacaaacgcc ataatctggt
    18121 cggagttaat aaaggagaaa gacccaagat agacatcaat cccgccacat ccggcgttga
    18181 tggacggcag gctgatggat atcagctgga tatttttgtt tcctgtgcgg gcatagaacg
    18241 aaccgccggt tacatagcgt gccgcctgtc cctgccatgc tttcgctgag gtggcgttgc
    18301 tggcatagct catattgtta aaaaacgaat tcatgtcatc ctgcagcccg gcactggcgg
    18361 acgtgcacaa cgcagtgcca atgagcagcg agagaaatga ttgtctcagg aaagttttca
    18421 ttgctccccc tttcgtaaat aaagagggaa acgtccgaaa ataaatacct tcaggtactg
    18481 gacaacgcta atcagcgtta acatcacaaa gaaggtgacg gacactgaaa taaacaccgg
    18541 aggaataaga tacccccaca tattcaggtc agtgcgtaca tcttcaggca aagacagcca
    18601 gaactcactc tgagcagggg aagtgatcgc ttcaaagaca gctgaccaca gaaagcgtga
    18661 cacaaaggcg gcaagcaagg ccagccagaa ccctccccat aacctcctct gtaatatgtt
    18721 aaagatctta ctattacctg cactatataa cgttccatac aggcgatatt tttcattcat
    18781 cggactgata atatcgatac tgtccttgtt gagacattca acctgatatc cctgcccgac
    18841 taaaaaaaac acgatatcct cacgcccttc aaagcatccg gcctctgaaa ggatacgcag
    18901 agtacggtcc agagcatctg cagaagccgg gaatttattt tcagcagtca tcgccatctc
    18961 cttcattgtg ttttttgtaa agcctgacgg tccagggcca tacgcagtac gtcatcaaga
    19021 cgcttacgga cctctccggc ctctgcggcg ccctgcagca taggccaggt ttccatcgtg
    19081 ttaacgttgg tcagaaaggt ggtcggtgta gccaccggca caccgctctg gaaaaactcc
    19141 accatcacat ccggggtggc cggaagcaca tcgggaaatt cagcgtctcc ttttccgtca
    19201 aggcttaccg ggaagacact gagaccggtc tgctgcgaca gggacttcag caccggcgca
    19261 aactggcggc agtactgaca ggtcgactgc ataaacagca ccagtttcca gtcctgcaga
    19321 tttacccgcc gcccgtcact cagcggatac cagatgggcg acgccttttc gctgagtcct
    19381 ggtgatggtg gtgtgttgct ctcaggaaga cgcagcccgt ccagtgcagg tgcactacgt
    19441 ccgttttttc cggcttcaag ttcacggata tcatccagtg tcccggccag cagacagccg
    19501 gacagcatcg ccgccatcag gatggcggtt cgcaatacga gaataagcat ggattacctc
    19561 agaaaaggcg ttatcagtag tcaggtttga aatgggtaag aacgttgacc atctggcgat
    19621 ccaggtcatc gtggctcata aacccccatg ccaggggctg ccactggtgc gttttcggat
    19681 cgaccaggaa agtcgccggg aaatgcgtgg cgcgcatctg ttcagcctgt ccgctgtccg
    19741 ggcgactctg cgggagctga tcgctgattt taccgtccac gctgacggcc atgagcgtga
    19801 taccccggtc ttcgcagaac gcgcggatga caccggccat caggttgtcg acggcattat
    19861 tcccccggta gaagaagaac agaccgtagc gctgcgccac ctgacggatg accgcattct
    19921 gaaccttcct ttcttcggta agacgcgctt ccaccgaacc gttgtaatgg ctgcgcttca
    19981 cgttgtagtc aagctccgga tatttcaggc gcgccagctt tgatgtctgg gtaaaatcgg
    20041 tcgcccggtc agtccagaag ttctgcaaca tcataaagcg gcggaagttc tccgctgagg
    20101 gatacaggat ggccgtatcc agcgcttcac gggtcgcctg ctgcaggacc tttttctgct
    20161 cagatggcgt catgctaacc agacgttcct ggagggtcgg cacctgtgtg gccccggact
    20221 cagtgtcttc cgcattcggg ttgtaccact gccagccttc ttcccgggca tagttggggg
    20281 tcagtacatc atcgggagcc gcatgcagtg atgcagttgc aagaagaaca agcacaccgg
    20341 cgacagctct catggtgcgt tctccttcat cttttcagcg ataatgtcct gtgctttctt
    20401 caacaaggcc tggtcttccg gaatatcgct gccggcattg agatcatcat agaaatcgct
    20461 gaagttcatc acgccaaagt ccagcttctg cagttcatcc accgtgatgc cccgacagtc
    20521 cggagatttg ccactgccga aaccaatgcc cagctggcca cggcgcccct gctcctggac
    20581 aatgcgtgcc agtttggaat caaacacgca gtaaccgcgt ttcttctcca ggcatacacc
    20641 cagcactttt ttactgcagt attccccgac atagaccgtc agttttttct ctttagcctt
    20701 acccagtgcc ttttcatcgc tgctgcagct ggacagtccg atatcctgcc cccagccaga
    20761 atccttacag cagttattga aacccgccgc catctttttg catgattttg ctgtaccggt
    20821 gaatgccctg acgttttcgc cattcagttc agcgacgtct ttaccggcgg ttgcgactgc
    20881 ggccagcgca gagacggcct gcccgaacat attgctggtc cccgtctgtg cctgggcgca
    20941 actgccgtct ttacagaaga actccccgcc gcaaatctgg ccgtttccct cttttttacg
    21001 ctcacaggag taggtgacct gctggctgac acagaagcca tcgacctggt cggcacactc
    21061 acttcgggtc accgtgcagg cagggttctt catatactca ccgcaggttc cctcggtttc
    21121 ggtctgtgtc agatatgtgt cctgccactt ccagcagtcc tggtggatgc tgtaggtttt
    21181 tccgtcaaca acgacggtcc gggtgcctcc tggctctaca cactgagatc cggtcattgc
    21241 accttcagac ttgctgaacg ggcagctttc tgtccagaca acctcaggta cccattttct
    21301 ggaaggaacc tgaatcgtca gcgtaatggt gaaacggtac ttcatatttg agctatacca
    21361 gatggaagac actgtaggtt tgttgcgtct attcgttatg gaaaacggca atgactgccc
    21421 atctgttata ttcagcccgg aactcagcgt gacgctccct tcagaatcat ccattgaaat
    21481 taaaccaaat ggcgtggtca ctctcatgaa ccagtgatcg ttgccgtaag caagtttctt
    21541 attccaggag taaaccgcgc tggcagcgat cactttccct ccagtgctga ccttaaattc
    21601 accagaaaaa gaattgttct tttcagtgaa ccgaatcgag gaagattcaa tcactatctg
    21661 tttgatttct gtcgtatcct catagtggcc tgtgattgtc gcggttcgcg tgcagtactg
    21721 ctctatcttc ggatcacgca agcactgatg cgtggtggtt tcggtaaaac tggcaggctg
    21781 gtccacgcag ccggcaaatc ctccaccagt aatggtctcg gccttatcct tcatggccag
    21841 cccttctgaa ataaacggcg catccagcga cggcttgtta tccggcggcg tattgagaat
    21901 ggactccgtg attattttac ccgcttcaga tgtgttcagc gctgctgaac ccggggcggt
    21961 catatccaca ccggaagatg tcacaccacc gtaataccca ctttccggcg gcgagtcggt
    22021 ataaccgggg atgacctccg caggattaaa cccctgaatg gcactggccc cctgtccctt
    22081 attctggcta ctcagattca ttccctggtt aaactgctca tttgcgacaa gcggctgtac
    22141 caggcagaaa gatgccgcca gcatcagtaa aggaaggtat cgtttcattc tccctcctca
    22201 ccaagcaggc tcctggcggt gtcagcacag tcgccctctt ctgccacccg cttcagggcc
    22261 tgatgcagcg taagatcccc ctgcagacga tcgactttct caccctgatt accacaggct
    22321 acaaccagca ccggcacggc ggtaatgttg tatttacgaa acgtcgtcgg atctatctgt
    22381 accccaccgg tattgctctc tttcaccaga ccggcaacgg cattagccgt cgcccgcata
    22441 tcgccgttaa ccattccccg gataaccacc ggaatattga gttgtgctgc atcctttaca
    22501 cgctgcttca actcctccgc cggcatcgag aaggacacaa acaccagggc cgtattcacc
    22561 gggcgttccc cgggctgcat ggattgccgg aactgctgtg cctgcgcgtc gagaaacgac
    22621 tgctcctgcg gtgccaccgg acgggtctgc ttcaggaatt ccggggtttg cagagactga
    22681 aatcgctgac ggtccagttg cagctgctca tggataaact gacggttctc cgtatgatta
    22741 ttcgccatgc cgcccccgct gaccagaagc agggccagcg ataccattac tcgtttcatc
    22801 gcgtttcctc ctgtgacggg cggtgtgcca taccgtaggc gcgatccgca gaacgaatgt
    22861 ccagcatctc ctcccacatg cgtcgttccc ggtctgacat ctgccgtctc agtaccggaa
    22921 gcagaccgct tatccatctg tccacaaacg ccagacgctc cagtgtcagc catacctgtt
    22981 cactacctga cgatgacagc gccatcgccg cttcccggca ctccagaata catttgaacg
    23041 gcccgccatt tttctgtagc gcgccgttca gctctgcctg ctgacgctcc agcaggtcag
    23101 cacgggaggc ggcctgttcg tagcgggcga gcagaaagtc atactttttt ttactcacga
    23161 gcatactgtg ctccttacag aaagacgcag ttgcgcttac gccagaagag atacccaaag
    23221 ttacgccggt cattgggcat gttcttgccc gcttcccagc gggttacgct acgccccagc
    23281 ggatgacact gtccggcatc gggatacttg ttgaccatgt gatatcgcca gcggcttttg
    23341 gggatgatcg gggatggata ttcaaagcag acagccctgt tttcaccgat gctgttcatg
    23401 atcatgccct ggcggtgaag cttgaacgcc atgcgctcac tgagtaacag tgacgtctgt
    23461 aacggactga attcgttaga aacatagccg ttaaacgggt acatactgcc ctgactgccg
    23521 gcacaccaga aaagggcgtc gatgggttta ctcaccagac tggccacggc atcagcggca
    23581 caggctccct gggcgatgat gttgccaaag agaatcgctt ccggctggat aatcatgctg
    23641 agagagctgc tgtcccacat gggatcaagt tccgacagat aaccaatatc caggtcgcca
    23701 ccctgcaggc agcccatcga cgtgataatg ttcagccagt aggtcaacgg gtatttgtac
    23761 cagtgaacat ggtagaaggc ccccggttta gcgcggcttt cctgaccacc actgccgctg
    23821 ccgacattcc cgatattgat attaaagccc cccagattca ccatgcagta aggggagcgt
    23881 gtcacatccg tcagcgccat cggctcccag taaccaatgg ccagccccac ccggtataaa
    23941 atgcccatcg ggcaaaactg gatcggcatg gaaggattcg gcgtatccgg ctgacgtcct
    24001 ttggcgaccg gaacattgcc aatggttacc ggaaaaatac actcccagca gatatccgtt
    24061 attggattga caaaattgcc ttcacattca gacgaagcgg ctctggccgt cccggtcagg
    24121 cataacaaca tcagcgccag tcctgttaca taacggttca tggcttcacc tccggcacat
    24181 ccagtgcaaa cgaatccacc cgcagacggc gacgatcttc cgccagcgtc acacgggccg
    24241 ggatggtttt gagttcaaac ttccggctga gcgagccatc ctgatcgaag taaatgcgtg
    24301 tattcagggc attggtcgct tctcgaacat ccccattgac cagtatgacg cggtacgtca
    24361 gcgtgccggg attctgcttc ttcatccacg ccagctgctg cggattatcc gcatcaataa
    24421 aatacagcgt gtcggtaaag ggcaccgtat caagcggatt aatcacctgt cccttacggg
    24481 caaacacccg cccctgatgg tctttcagat cttcactgac cgtcagcgat ggatcgatat
    24541 agtgcgtggt gttttcctgc gccagcgtca gcccttccac cggcttagga cgcagggtgt
    24601 tttctatgac acgctgtttg aatgcttcct gctcccgggc catctcacca ctggcctcca
    24661 ttgctttcag ccgggtctga atggtggtca gcatgtcctg ctcggcaatg gggtacatct
    24721 ccccccatgt gcccaggttt tttgcctgta ccatgccgga cagggcaagc agtatgagca
    24781 gacgtcggta cattactgcc cccctccggc ggccattttt tgtgccactg cagactgaat
    24841 ttcaccggta atgtctgccg caccgctgac caccgccggc gtcaccagga taaggacgtt
    24901 gtgatcgcgc tggtattcct gaagttctgt gctaagtgtc tgcataaagc gttctgtcag
    24961 gacgctcatc tgcgcctcat tcagggactg tgcccccgcc tggtcggtga actgatcgac
    25021 cgtgcccttc atatcaaaag acacaaccgt cggtgtacgc cagctaatca gcagagacgt
    25081 gaccgccgcg ttaacgcaca gaacagtggc agcgaccagc agcagattac gcagacaacg
    25141 gcgacggcgt tgctcccgcc ggctcccttt aacattaata ttcccccctt ttctgaatga
    25201 ctcagcagaa acagaggatt cagacggtgc tgagttaagt ttttgctctg tggtcatgct
    25261 gcctccgcaa gcgcttccag ttcgcgcatc tcatcaggga agttgcgttg tgccagctgg
    25321 tagacggcgt catggatatc catgttctgt tcccggcact ggcggataaa ttcgaagtca
    25381 tcacctttcg aactgaacat cgcccggctg agcgggtcga caaagagacg atggaaactg
    25441 caggtatcat tgatgcgcag cataaaggag gagaaccact ggtctttggc atccccgaat
    25501 ttagagataa cgctgcgctc cagctcgctg aactggttag ggcggttctg gttgtactgt
    25561 ttgaattccg acgcatcctg cttgagaacc accttgaagg cagagttacc ccaggctgct
    25621 tttgctgcgc cggaggcatc atcggcatca aagtccttga tgttctgact tatggtgatg
    25681 aacgcgccac ggtgacgtcg cacagtgcgg tagccggtct caatgaactg accgaccttc
    25741 tcatttttga agttgagcag cttccagcct tcgtcgatgg tgcagcactt cttcaggctg
    25801 cgcggcgtgc ggtacatctt gtcctcgatg tagatgatga gggagaacat caccgcaacc
    25861 agcagatcag gcttgtcctg cagaccgccc agctccagga cgataaagcg ggcatcgtcg
    25921 gtcaatgacg gctcatcgct gttgaaatat tcaccgtaaa ttccccagga gcagtatttc
    25981 ccgagcagct cgataatttc atccagtcgg ttggtgatgc ccggcgaatt ctgatattta
    26041 tcatgctcac ggatagccgt cagcttatgg acaacatggt caatacgggc agtacccctg
    26101 tgttcttccc aggcctcctg aaccccgcgc aacagcaggc tgtggtggac ttcatccagc
    26161 gtaccgttag ggcttgccag cacagccagc tggtcacgga tacgttcccc ggactcattg
    26221 atgttagaga tgttggcaaa cggattaaat ttgagtgact taccgtcaag gtaggtgcca
    26281 cccacgttct cacagaacga tttgtagccg tcccccatgt caaacaccca ggcgataccg
    26341 ccggagtcca gcacgctgcg caggataggc tgcaccaggc cggtcttgcc tgcccccgag
    26401 gtaccggtga ccgccatgtt gtagttagtg ttgcccatcc cgtccccgta gatatcgagg
    26461 aaggccagct ggttacggta ggacggtgtc agcagtcccc cctgacacag ccggttgtcg
    26521 gccaccaccg gcagcagatt cacggcctgg atactctccg cccggcacgt ggctccgccc
    26581 attttaatgt cctcccagag cccttccgca tgaatgaagg ggatcatcgc tatccagttg
    26641 cggaactgca tataggtcgg ggaaaacagc tctatgccgt tcttcttgaa cgtattaatg
    26701 acctgctgct cgcagaccag cgcgtcctca tccgtgtccg gacagaacgt ggtgatgttg
    26761 tagtaatagc ggacaataca ggtctggttg ctgttgagac gctcacgaat gtccttccac
    26821 tcctgggcct gtttggccac accgggaaac agtgtggcat agggggattt cgcttttttg
    26881 tcccgatcga gatacttgcg cgtggcctcc ccctgcgttg ccacctggtc ctcggcctcc
    26941 attaccatgg tgatgacgaa cgggctggca atggtcaggt ccgggctgag cagattggat
    27001 aggttatcgc cgcccatcca cagcatgaac atatccgggt tattttccag cataaagttc
    27061 atggcccgtg ttgtggtcac actgccctgc ctgtcagcca ggctgatacg cacatcgtca
    27121 ggacgcacat cgataccgat acgctgatca atacagcgct gatgcagttc atcaaatttg
    27181 ccgacacgta cgtcgtccgg atacaactga tcatggcggg cattcagcat cccgtgaacc
    27241 agcgtggcca gttcctgagc atctgccgtc tgtgtgaaaa tttttgccgc atccagtgaa
    27301 gaacgaagaa ctttcagtgt gtgggcaacc tccgtcatga ccgccgccgt acgtttcttt
    27361 gctttcacac agtaggagat atacaggcgg tagtcccgca gggtcagcgg caaccccgta
    27421 ggactgttga actgctgctg tgcagcacgc cggtaaaacg cccgcgtgat acggttaaat
    27481 ttatccgcac gcttaccctg ccagcggaat tcagacaggc cacgatccag ctgctcgcca
    27541 atcagcttac tggacaccag gtggaaggaa atcggtgtct tacgcggcag cttgcttttg
    27601 atgaggtctt ccagcacata cactatctgt tcgttagcac cgataagggg ttccgcccgc
    27661 agcagaaaac caaccgtact gcggttcaca aacagcccgg agtcacggtc atagtcgcga
    27721 tacggcagga cgctggtcag ctgcggaaaa tccagactgg ccagattacg cgtcatctca
    27781 gatgcaccat ccggcatacg gaaagcattc gtgaggttct ccagcacgct cagaaatttt
    27841 ttcaccagaa cctccttaat ttatgcgggc cggaaggacc cagtcagcag ggctgaccac
    27901 aaaagagaca cgaccgggct gatggaaagc gttatcgctg tccacccagg gcgcgatcca
    27961 gacggtggct atgcgatccc ggctgcgctg aggatgtaca gagccgggat tatcacaacg
    28021 cacggtctgg catgagggag ccggattgcc cacggacaca gccggacgcg aaactggtgt
    28081 taccggcgtt ccagcaacca gacggggcgt cagggttggc gctgcggcat tcgtgctttt
    28141 acctgttgcg ccgtcaactg atactgcagg tttgttgctg gttacctgta tacgggaaga
    28201 ggtgctgaca gcagagcggg atgctgacgg aagcaccgcc gcagaagtgg ccgggagatt
    28261 aaccagggaa ggcagtccgc ccgcagccgg ctttcccgcc tgttttgccg ccttgtcccg
    28321 tgccagctgg ttggctttcg tcatggtcat acaggtatca gacgttgtgg catcacagtc
    28381 aaagctgctt ttaacgccgg cacagccggt caacagcagg acactgcctg ccagcaaaaa
    28441 tgtgacattt ttcatcgctg tttcctgaaa attatgctct gaagagtctg gctggctgcg
    28501 aaagcgcagc actttattta tcatgacgca cgtctcctgg cttcagtgga taccagatgt
    28561 tacagttctg acggatagcc tctgccagcc tctcccgtcc tcaccgctat acagtgcttt
    28621 aatctggctg tcgtcagact tcgccaggaa aacccgggac atcggttgtt tggttatgct
    28681 catacctgcc cccggagttc ccgggaacag gcagtgccct cgttcccgct gcagtacaag
    28741 tacgtcctga gaaatccgcg acttcccctc aaggacggta gcgcacggca gagacgctga
    28801 gaggcagaag tcgctctctg cgtcatcaac acagatgaca cgcatccccg gattctcaca
    28861 gcagaccgca atatcattaa ggtggcggat cttacccgga gcgccgttac tgttggttgt
    28921 catgatcttt atctcctgcg cttttgccgt ctttctgaat gtttttgcgc aattcacgcc
    28981 agtcacggag ttgttgttct gataaaaagg tccgcataac atcaacgctg atgactgtct
    29041 taccgctacc ggttttcccc acgcccatca cattggtttt tctgtttttc atccttcacc
    29101 tcaggtagtg tttctgccgg atttccagta cccgctggca ttccgtgcag gttcgtacac
    29161 caggtatcgt ttgctggcgt ttgagaggaa tatcgttgcc acaacattca cactcgcaga
    29221 ggctttcccc accccttatg atcaactgcc gggcagcctg aatatgcagc cggttcagca
    29281 ggtcttcctg cttctgtaaa tggtccagaa cgtcggccat cggttctcct tgtttacgct
    29341 gtccggacac gctaccgccc atccctttca cgtcgttcag ggcactgact gttccggatt
    29401 gtgttgttct ggccggtatt ccggctcccg tttactgcgt gacaacctgg cctgtagccg
    29461 ggtcgacggt gtcccccagc ctgaagtcct gcaggtgttt aagcatgtcc gtcgtgttgc
    29521 cgggcatggc agcaggcgca gatgaggctg atggttgatt ttgtttcctg cgtgcagcgg
    29581 ctttcgcgcg ggcttcttcc agcgtttcaa gctggaagcc atcctggaaa accagggtga
    29641 cttcattgcc cgcaccaatc ggaataaccg ggtggtattg ttcagcacgt ttgatgtagt
    29701 aatcagagag tgttttggcc gcagaactga ctccgccgcc gagcccggcc tggccgattt
    29761 caccggcact catactggca gtggctccaa cgcccacggt tgtggaagag gctttctcaa
    29821 tgcccttccc gataccatca agaaagcctg atccccccgc atacagcagg atttgaccgt
    29881 tacgcatgac cacttcgccc ttgatgccgt tcttgcccat aaaggacaca tgaccggcaa
    29941 tcttctggtc gatattgtca tcacccagct tgcagctgat ggagcgggtc cggacgatgg
    30001 cgcgttcact ggaaacatcc ccccaggctt cgagagtcac aaaacacccg gttagatcga
    30061 attcctgatc gttaggcatc tgaactttgc cggtcaggcg aagctgcata gggacagtat
    30121 ttttatcgcc ggtgacagag gcattggcat cagcgccttc caccacaatg gcttcggcaa
    30181 aactgccgga ggaaatccac gggaaacggg gcttttgcgg agcggtgtcc ggctgagtga
    30241 attcatggct gtccatcatt cccggacgct gaaccggggt catcaccggt gcgacaggag
    30301 cctgtccgcc accggcgggc cagaaagtgg tcgggggagg aacatcaccg gcctgtgcag
    30361 cagtttttcc ctgtgaggcg agcgcccctg gcagcggttc agcgctgaga gagtcaaaat
    30421 tcttcccggc ttcgatttgt gtctgcagga gtttattttc atcttccagc tcacggatac
    30481 gctcctgaga gcctttcaga tcacgcccga taattcccat ttctgtacgc agctttttaa
    30541 tttcagcctg cgtttccttg ttcagacgct gggcttccgc aatggcggaa cgctggactt
    30601 tattgtcaaa ggactgattg accaccccgg tcatatccgg cgcaggttcg ccttttgggg
    30661 cttttgccgg cggggctttc ttcaggttca tctcggatac catccaggtt cctgccccac
    30721 ccgcaccaat acccagcaca agcgcgataa acagagcgag ctgacgacga cgaacgattt
    30781 tgttaacgtt ggccatctgt tccctccccg ttttcacgga taacggtgac agtcagccgt
    30841 cccccgccca tcagggtctg tgcattgctg tcaaacatga cggcacgaac accgggcttc
    30901 cagaagtcct gctcacgcag agccaatccc cacgtattgg cattgcgcag ttcataacga
    30961 tcgacccgta actggtcgcc cgcccatgct ttcttcggcg tggcggaaaa accacctggt
    31021 acgttgatac cgtctgcaag cggtttcacc tcgccatagc catcaggcat tttgtcggtc
    31081 agcacagacc gggtgagtgc gatgagcagt ttttcgtaag cctgggcggt ttcccactgg
    31141 cgggtttcgg gccgcgacgg tggttctgcg ctcatcagtc ggtagactcg cccctcacct
    31201 ttgacgggcg tggccacgac cgagaaggtt tgtcccaggg cagtttccac atacaacgtg
    31261 aaggcccggg tcgccacgga ggtgaacagc accccgccgg cagacgtcag tcgtttatct
    31321 gccagcatgc cgttctgggc tgtgatggcc gtgactttat ctcccggaat gaaaatcata
    31381 ttgggatcgg tattactgat actgagccgg aactgccccc cctgcggaaa gctgagtgtg
    31441 gcaggtgact gtgcatatac agtcccggac gaagtgacca tcatggcagt gcccaaaagc
    31501 agcgccggaa agttattttt cattggttac ctcatagaaa cggccaatgc gggtaaagcc
    31561 accggcgtac ttaaggcgga gacgatagtt ttttaattcc gtggtgggtt ttgagttgcc
    31621 gatccacatt ttcagcacgc ccctgacatc cacgcgcccg tcaacgggat agactttgat
    31681 ttccgtggtg tagaatgtgg aattcacatc actggccttt atctgcgcgg cctcttcctg
    31741 cagaactttt ttaaattcct ctctgacttc tggttcgaca aaagacatca gaaaagcatg
    31801 gttggaatct acagtttcgg gggagacgtt aagacgcagg gccaggaagg acagcgtcat
    31861 catccggaaa aactcggtat ccgcttttgt ttcagatacc gcgaaaggcg cgttataggt
    31921 catcggggtg acgaccgtac gctgctcatg aatgagtgtt ctgttagtca ccgccagcca
    31981 ggtaacagca aatcccagaa taaagaccag agccgtgagt gacagaataa taatggcaat
    32041 aattttggtg gatgagttac gagcgcttat ttccataaga tggccctgca atattttact
    32101 ttatccattt cctgtaactg gagtcgggaa taacgcggta gacaacccgg aataattcaa
    32161 caggcagatg ccagtaacac cagttatata accagcggga actctttccg cgttttgccg
    32221 cccggattag ctgccagacc actgtcgcaa taatcagacc aaatacatat ttcttggcga
    32281 aaaatcccca cagcactaac acagctgcgg ggatggcttc atcaagtggc agtccgataa
    32341 tgcggttctg ctctgacaga gttttcggga aacggaattt ttctaaatcg tttcccgaca
    32401 tatcagctga cgaatgtcag gccaacggtg gtgaagacaa taaccacgac gagtcccagc
    32461 agaatcagca ggttcttggt tcggatatac gtcgcaacac cgataacgat ttcagcgata
    32521 taaatacaca tgatgacaaa tgagtcggca ccaaaggtcg cttttacatc gtccttgccc
    32581 cccgccagca ggtcagtggc atgggccatc tgccccatac ccagtaacag cagcggagag
    32641 aacgcagcca gacgggacag tgctttttgg ttacacactc cacgccatgc catctggcaa
    32701 taatgccatg cacggttttt tacagacgcg gggcggccgc ctttagataa ggataaattc
    32761 ataaaaactc catttatgtt atttacaaaa aacccagagc ggtattgaca ggttaagtca
    32821 atccggcaca gaaccatcaa aaactataaa tggtcctgta acagatgtct taaccgacgt
    32881 gataattttt attatcttct tcctcgaaat aagaactgat aatttgttca gccatctttc
    32941 caatcttcat cttcgaaaaa atcgcgcttt tctttaactg ttcataagtt gtcatatcca
    33001 aaaatacatg cacctgcttt ctgacaggcc gaacaacatt cacatcgtca ctcctcatat
    33061 gtcctccgaa gatgattcag ggctgggaaa atcgtgacaa aaaaataaaa aggcatcaac
    33121 ttgtataatt caacctgaaa aaattcgata taaatacatg atttttaatc gaaaaacaga
    33181 cttttactgg aaaaacccta caaccataaa ttgatcaata tcaaagataa agtgtgatgt
    33241 aaatcacatc acacttttga gagatatcac ttaagagtgc tcttcataac taaaacgaag
    33301 gtggttatag agatcgagtc tcaccgtgta gacagtttta agcatatctg tagtaggtac
    33361 agcatacact tcacataatt cacgtaagtt tttggatatg gcttgaacac ttacaccttt
    33421 atagtaagca atgtcttttg tactcatccc gtaaacaaaa aatgtcaaaa tttctatttt
    33481 ttgatcactt atttcaggaa agatcttgct taatttagac ataaagatag gattgataat
    33541 aaccatcgta acctctggtt taaaatgaac aggttattgg tggccagcga tagcaggggg
    33601 ggccaacccc tgttatcgcg acagtagcaa atcaatttta taaatgcatt caactccctg
    33661 ggaacaattg atttacctta agttgaatat acaacatca
//
LOCUS       DBFIFI010000030        32748 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.030, whole genome shotgun sequence.
ACCESSION   DBFIFI010000030 DBFIFI010000000
VERSION     DBFIFI010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32748
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.030"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..198
                     /locus_tag="N7B39_001392"
     CDS             <1..198
                     /locus_tag="N7B39_001392"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2421400.1"
                     /translation="INNSNGMFLSALHYFFAIVNYSVNYGNDEAQRENPAIFPYFQSC
                     DRMSLMMLITLTLQNGPYERI"
     gene            176..967
                     /gene="nanR"
                     /locus_tag="N7B39_001393"
     CDS             176..967
                     /gene="nanR"
                     /locus_tag="N7B39_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HGU2421401.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            1089..1982
                     /gene="nanA"
                     /locus_tag="N7B39_001394"
     CDS             1089..1982
                     /gene="nanA"
                     /locus_tag="N7B39_001394"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HGU2421402.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            2091..3581
                     /gene="nanT"
                     /locus_tag="N7B39_001395"
     CDS             2091..3581
                     /gene="nanT"
                     /locus_tag="N7B39_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HGU2421403.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCTAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            3629..4318
                     /gene="nanE"
                     /locus_tag="N7B39_001396"
     CDS             3629..4318
                     /gene="nanE"
                     /locus_tag="N7B39_001396"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HGU2421404.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLTLVKRLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            4315..5190
                     /gene="nanK"
                     /locus_tag="N7B39_001397"
     CDS             4315..5190
                     /gene="nanK"
                     /locus_tag="N7B39_001397"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HGU2421405.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLGQLTDLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADAKTIFMRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            5187..5651
                     /gene="nanQ"
                     /locus_tag="N7B39_001398"
     CDS             5187..5651
                     /gene="nanQ"
                     /locus_tag="N7B39_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HGU2421406.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLKGEERILFGTAGTARQCEEFHHEDDYQ
                     LCSAIENEQTINLKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(5711..6760)
                     /gene="yhcG"
                     /locus_tag="N7B39_001399"
     CDS             complement(5711..6760)
                     /gene="yhcG"
                     /locus_tag="N7B39_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HGU2421407.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLPWSTYVRLLSVKNADTRSFYEKETLRCGWSVRQLERQIATQFYERTL
                     LSHDKSAMLQQHAPAETHILPQQAIRDPFVLEFLELKDEYSESDFEEALINHLMDFML
                     ELGDDFAFVGRQRRLRIDDNWFRVDLLFFHRRLRCLLIVDLKVGKFSYSDAGQMNMYL
                     NYAKEHWTLPDENPPIGLVLCAEKGVGEAHYALAGLPNTVLASEYKMQLPDEKRLADE
                     LVRTQAVLEEGYRLR"
     gene            complement(6944..8362)
                     /gene="gltD"
                     /locus_tag="N7B39_001400"
     CDS             complement(6944..8362)
                     /gene="gltD"
                     /locus_tag="N7B39_001400"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HGU2421408.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVQAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TREEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHSMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            complement(8375..12835)
                     /gene="gltB"
                     /locus_tag="N7B39_001401"
     CDS             complement(8375..12835)
                     /gene="gltB"
                     /locus_tag="N7B39_001401"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HGU2421409.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNEI
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHNDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTET
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            13509..14438
                     /gene="yhcC"
                     /locus_tag="N7B39_001402"
     CDS             13509..14438
                     /gene="yhcC"
                     /locus_tag="N7B39_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HGU2421410.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            14534..16870
                     /gene="arcB"
                     /locus_tag="N7B39_001403"
     CDS             14534..16870
                     /gene="arcB"
                     /locus_tag="N7B39_001403"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HGU2421411.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVNVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            17100..17753
                     /gene="elbB"
                     /locus_tag="N7B39_001404"
     CDS             17100..17753
                     /gene="elbB"
                     /locus_tag="N7B39_001404"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HGU2421412.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGRECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            17750..18478
                     /gene="mtgA"
                     /locus_tag="N7B39_001405"
     CDS             17750..18478
                     /gene="mtgA"
                     /locus_tag="N7B39_001405"
                     /EC_number="2.4.99.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HGU2421413.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDRVSMDQISPWMGLAVIAAEDQTFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSAPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(18475..19107)
                     /gene="yrbL"
                     /locus_tag="N7B39_001406"
     CDS             complement(18475..19107)
                     /gene="yrbL"
                     /locus_tag="N7B39_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HGU2421414.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDVAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALASREA"
     gene            complement(19321..19593)
                     /gene="npr"
                     /locus_tag="N7B39_001407"
     CDS             complement(19321..19593)
                     /gene="npr"
                     /locus_tag="N7B39_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HGU2421415.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            complement(19590..20444)
                     /gene="rapZ"
                     /locus_tag="N7B39_001408"
     CDS             complement(19590..20444)
                     /gene="rapZ"
                     /locus_tag="N7B39_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HGU2421416.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            complement(20490..20981)
                     /gene="ptsN"
                     /locus_tag="N7B39_001409"
     CDS             complement(20490..20981)
                     /gene="ptsN"
                     /locus_tag="N7B39_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HGU2421417.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTKGTP
                     DEA"
     gene            complement(21099..21386)
                     /gene="hpf"
                     /locus_tag="N7B39_001410"
     CDS             complement(21099..21386)
                     /gene="hpf"
                     /locus_tag="N7B39_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HGU2421418.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            complement(21409..22842)
                     /gene="rpoN"
                     /locus_tag="N7B39_001411"
     CDS             complement(21409..22842)
                     /gene="rpoN"
                     /locus_tag="N7B39_001411"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HGU2421419.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            complement(22890..23615)
                     /gene="lptB"
                     /locus_tag="N7B39_001412"
     CDS             complement(22890..23615)
                     /gene="lptB"
                     /locus_tag="N7B39_001412"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HGU2421420.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            complement(23622..24179)
                     /gene="lptA"
                     /locus_tag="N7B39_001413"
     CDS             complement(23622..24179)
                     /gene="lptA"
                     /locus_tag="N7B39_001413"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HGU2421421.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            complement(24148..24723)
                     /gene="lptC"
                     /locus_tag="N7B39_001414"
     CDS             complement(24148..24723)
                     /gene="lptC"
                     /locus_tag="N7B39_001414"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HGU2421422.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            complement(24720..25286)
                     /gene="kdsC"
                     /locus_tag="N7B39_001415"
     CDS             complement(24720..25286)
                     /gene="kdsC"
                     /locus_tag="N7B39_001415"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HGU2421423.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            complement(25307..26293)
                     /gene="kdsD"
                     /locus_tag="N7B39_001416"
     CDS             complement(25307..26293)
                     /gene="kdsD"
                     /locus_tag="N7B39_001416"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HGU2421424.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHIPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            complement(26307..27284)
                     /gene="yrbG"
                     /locus_tag="N7B39_001417"
     CDS             complement(26307..27284)
                     /gene="yrbG"
                     /locus_tag="N7B39_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HGU2421425.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            27494..28303
                     /gene="mlaF"
                     /locus_tag="N7B39_001418"
     CDS             27494..28303
                     /gene="mlaF"
                     /locus_tag="N7B39_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HGU2421426.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            28311..29093
                     /gene="mlaE"
                     /locus_tag="N7B39_001419"
     CDS             28311..29093
                     /gene="mlaE"
                     /locus_tag="N7B39_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HGU2421427.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            29098..29649
                     /gene="mlaD"
                     /locus_tag="N7B39_001420"
     CDS             29098..29649
                     /gene="mlaD"
                     /locus_tag="N7B39_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HGU2421428.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            29668..30303
                     /gene="mlaC"
                     /locus_tag="N7B39_001421"
     CDS             29668..30303
                     /gene="mlaC"
                     /locus_tag="N7B39_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HGU2421429.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQRDAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            30303..30596
                     /gene="mlaB"
                     /locus_tag="N7B39_001422"
     CDS             30303..30596
                     /gene="mlaB"
                     /locus_tag="N7B39_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HGU2421430.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            30756..31010
                     /gene="ibaG"
                     /locus_tag="N7B39_001423"
     CDS             30756..31010
                     /gene="ibaG"
                     /locus_tag="N7B39_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HGU2421431.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            31065..32324
                     /gene="murA"
                     /locus_tag="N7B39_001424"
     CDS             31065..32324
                     /gene="murA"
                     /locus_tag="N7B39_001424"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HGU2421432.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNTQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(32372..32650)
                     /gene="sfsB"
                     /locus_tag="N7B39_001425"
     CDS             complement(32372..32650)
                     /gene="sfsB"
                     /locus_tag="N7B39_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HGU2421433.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
BASE COUNT         7527 a         8345 c         8841 g         8035 t
ORIGIN      
        1 ataaataatt caaacgggat gtttttatct gcgttacatt attttttcgc aatagttaat
       61 tattccgtta attatggtaa tgatgaggca caaagagaaa accctgccat tttcccctac
      121 tttcaatcct gtgataggat gtcactgatg atgttaatca cactgacctt acagaatggg
      181 ccttatgaac gcatttgatt cgcaaaccga agattcttca cctgcaattg gtcgcaactt
      241 gcgtagccgc ccgctggcgc gtaaaaaact ctccgaaatg gtggaagaag agctggaaca
      301 gatgatccgc cgtcgtgaat ttggcgaagg tgaacaatta ccgtctgaac gcgaactgat
      361 ggcgttcttt aacgtcgggc gtccttcggt gcgtgaagcg ctggcagcgt taaaacgcaa
      421 aggtctggtg caaataaaca acggcgaacg tgcccgcgtc tcacgtcctt ctgcggacac
      481 catcatcggc gagctttccg gcatggcgaa agatttcctt tctcatcctg gtgggattgc
      541 ccatttcgaa caattacgtc tgttctttga atccagcctg gtgcgctatg cggctgaaca
      601 cgccaccgat gagcaaatcg atttgctggc aaaagcactg gaaatcaaca gtcagtcgct
      661 ggataacaac gcggcattca ttcgctcaga cgttgatttc caccgcgtgc tggcggagat
      721 ccccggtaac ccaatcttca tggcgatcca cgttgccctg ctcgactggc tgatcgccgc
      781 acgtccaacc gtggctgatc aggctctgca cgagcataac aacgttagct atcaacagca
      841 tattgcgatc gttgacgcga tccgccgcca cgatcctgac gaagccgatc gtgcgttgca
      901 atcgcatctc aacagcgtct ctgctacctg gcacgccttc ggtcagacca ccaacaaaaa
      961 gaaataatgc cactttagtg aagcagatcg cattataagc tttctgtatg gggtgttgct
     1021 taattgatct ggtataacag gtataaaggt atatcgttta tcagacaagc atcacttcag
     1081 aggtatttat ggcaacgaat ttacgtggcg taatggctgc actcctgact ccttttgatc
     1141 aacaacaagc actggataaa gcgagtctgc gccgcctggt tcagttcaat attcagcagg
     1201 gcattgacgg tttatacgtg ggtggttcga ccggcgaagc ctttgtacaa agcctttccg
     1261 agcgtgaaca ggtactggaa atcgtcgccg aagaggcgaa aggtaagatt aaactcatcg
     1321 cccacgtcgg ttgcgtcagc accgccgaaa gccaacaact tgcggcatcg gctaaacgtt
     1381 atggcttcga tgccgtctcc gccgtcacgc cgttctacta tcctttcagc tttgaagaac
     1441 actgcgatca ctatcgggca attattgatt cggcggatgg tttgccgatg gtggtgtaca
     1501 acattccagc cctgagtggg gtaaaactga ccctggatca gatcaacaca cttgttacat
     1561 tgcctggcgt aggtgcgctg aaacagacct ctggcgatct ctatcagatg gagcagatcc
     1621 gtcgtgaaca tcctgatctg gtgctctata acggttacga cgaaatcttc gcctctggcc
     1681 tgctggcggg cgctgatggt ggtatcggta gtacctacaa catcatgggc tggcgctatc
     1741 aggggatcgt taaggcgctg aaagaaggcg atatccagac cgcgcagaaa ctgcaaactg
     1801 aatgcaacaa agtcattgat ttactgatca aaacgggtgt attccgcggc ctgaaaactg
     1861 ttctgcatta tatggatgtc gtttctgtgc cgctgtgccg caaaccgttt ggtccggtag
     1921 atgaaaaata tctgccagaa ctgaaggcgc tggcccagca gttgatgcaa gagcgcgggt
     1981 gagttgtttc ccctcgctcg cccctaccgg gtgaggggaa ataaacgcat ctgtacccta
     2041 caattttcat accaaagcgt gtgggcatcg cccaccgcgg gagactcaca atgagtacta
     2101 caacccagaa tatcccgtgg tatcgccatc tcaatcgtgc acaatggcgg gcattttccg
     2161 ctgcctggtt gggatatctg cttgacggtt ttgatttcgt tttaatcgcc ctggtactca
     2221 ccgaagtaca aggtgaattc gggctgacga cggtgcaggc ggcaagtctg atctccgcag
     2281 cctttatctc tcgctggttc ggcggcctga tgctcggcgc tatgggtgac cgctacgggc
     2341 gtcgtctggc aatggtcacc agcatcgttc tcttctcggc cgggacgctg gcatgcggct
     2401 ttgcgccagg ctacatcacc atgtttatcg ctcgtctggt catcggcatg gggatggcgg
     2461 gtgaatacgg ttccagcgcc acctatgtca ttgaaagctg gccaaaacat ctgcgtaaca
     2521 aagccagtgg ctttctgatt tcaggcttct ctgtaggggc cgtcgttgcc gctcaggtct
     2581 acagcctggt ggttccggtc tggggctggc gtgcgctgtt ctttatcggc attttgccaa
     2641 tcatctttgc tctctggctg cgtaaaaaca tcccggaagc agaagactgg aaagagaaac
     2701 acgcaggtaa agcaccagta cgcacaatgg tggatattct ctaccgtggt gaacatcgca
     2761 ttgccaatat cgtaatgaca ctggcggcgg ctactgcgct gtggttctgc ttcgccggta
     2821 acctgcaaaa tgccgcgatc gtcgctgttc ttgggctgtt atgcaccgca atctttatca
     2881 gctttatggt gcagagtacg ggcaaacgct ggccaactgg cgtaatgctg atggtggtcg
     2941 tgttgtttgc tttcctctac tcatggccga ttcaggcgct gctgccaacg tatctgaaaa
     3001 ccgatctggc ttataaccct catactgtcg ccaatgtgct gttctttagt ggctttggcg
     3061 cggcggtggg gtgctgcgtc ggtggcttcc tcggtgactg gctgggaacc cgcaaagcgt
     3121 acgtttgtag tctgttggcc tcgcagctgc tgattattcc ggtatttgct attggcggtg
     3181 caaacgtctg ggtgctcggt ctgttactgt tcttccagca aatgcttgga caagggatcg
     3241 ccgggatctt accgaaactg attggcggtt atttcgatac cgaccagcgt gcagcgggcc
     3301 tgggctttac ctataacgtt ggcgcattgg gtggcgcact ggccccaatc atcggcgcgt
     3361 tgatcgctca acgtctggat ctgggtactg cactggcatc gctctcgttc agtctgacat
     3421 ttgtggtgat cctgctgatt ggtctggata tgccttctcg tgttcagcgt tggctgcgcc
     3481 cggaagcgtt gcgtactcat gacgctatcg acggcaaacc atttagcggt gccgtgccgt
     3541 ttggcagcgc caaaaacgat ttagtcaaaa ccaaaagtta atcctgttgc ccggtctctg
     3601 tatcgggcct ttcgctaagg gaagatgtat gtcgttactt gcacaactgg atcaaaaaat
     3661 cgctgctaac ggtggcctga ttgtctcctg ccagccggtt ccggacagcc cgctcgataa
     3721 acccgaaatc gtcgcagcca tggcattagc ggcagagcag gcgggcgcgg ttgccattcg
     3781 catcgaaggt gtggcaaatc tgcaagccac gcgtgcggtg gtgagcgtgc cgattattgg
     3841 aattgtgaaa cgcgatctgg aggattctcc ggtacgcatc acggcctata ttgaagatgt
     3901 cgatgcgctg gcgcaggcgg gcgcggacat tatcgccatt gacggcaccg accgcccgcg
     3961 tccggtgcct gttgaaacgc tgctggcacg tattcatcat cacggtttac tggcgatgac
     4021 cgactgctca acgccggaag acggcctggc atgccaaaag ctgggagccg aaattattgg
     4081 cactacgctt tctggctata ccacgcctga aacgccagaa gagccggatc tgacgctggt
     4141 gaaaaggttg agcgacgccg gatgtcgggt gattgccgaa gggcgttaca acacgcctgc
     4201 tcaggcggct gatgcgatgc gccacggcgc gtgggcggtg acggtcggtt ctgccattac
     4261 gcgtcttgaa cacatttgtc agtggtacaa cacagcgatg aaaaaggcgg tgctatgacc
     4321 acactggcga ttgatatcgg cggtactaaa cttgccgccg cgctgattgg cgctgacggg
     4381 cagatccgcg atcgtcgtga acttcctacg ccagccagcc agacaccaga agccttgcgt
     4441 gatgccttat ccgcattagt ctctccgttg caagctcatg cgcagcgggt tgccatcgct
     4501 tcaaccggaa ttattcgcga cggcagtttg ctggcgctga atccgcataa tcttggtgga
     4561 ttgctacact ttccgttagt caaaacgctg gggcaactta ccgatttgcc gaccattgcc
     4621 attaacgacg cccaggccgc agcatgggca gagtatcagg cgctggaagg cgatataacc
     4681 gatatggtgt ttatcaccgt ttccactggc gtcggcggcg gtgtagtgag cggcggcaaa
     4741 ctgcttaccg gccctggcgg tctggcgggg cacatcgggc atacgcttgc cgatccacac
     4801 ggcccagtct gcggctgtgg acgcacaggt tgcgtggaag cgattgcctc tggtcgcggt
     4861 attgcagcgg cagcgcaggg ggagttggct ggcgcggatg cgaaaactat tttcatgcgc
     4921 gccgggcagg gcgacgagca ggcgcagcag ctgattcacc gctccgcaca tgtgctggca
     4981 aggctgatcg ctgatattaa agccacaact gattgtcagt gcgtggtggt cggtggcagc
     5041 gttggtctgg cagaagggta tctggcgctg gtggaaacgt atctggcgca ggagccagcg
     5101 gcatttcatg ttgatttact ggcggcgcac taccgccatg atgcaggttt acttggggct
     5161 gcgctgttgg cccagggaga aaaattatga tgatgggtga agtacagtca ttaccgtctg
     5221 ctgggttaca tcctgcgtta caggacgcgt taacgctggc attagctgcc agaccgcaag
     5281 aaaaagcgcc gggtcgttac gaattacagg gcgacaatat ttttatgaat gtcatgacgt
     5341 ttaacactca atcgcccgtc gagaaaaaag cggaattgca cgagcaatac attgatatcc
     5401 agctgttatt aaaaggtgag gaacggattc tgtttggcac ggcaggcact gcgcgccagt
     5461 gtgaagagtt ccaccatgaa gatgattacc agctttgcag cgccattgaa aacgagcaaa
     5521 ccatcaactt aaaaccggga atgttcgccg tgtttatgcc gggagaaccg cataaaccag
     5581 gatgcgttgt cggcgaacct ggtgagatta aaaaggtggt ggtgaaggtt aaggctgatt
     5641 taatggctta aaacttagtt cgttttaaag attctgcaga cagtgtctgc agaattcgaa
     5701 accgctgaaa ttaacggagt ctatagcctt cttctagcac cgcctgtgtt cgaacgagtt
     5761 catccgcgag tcgtttttca tcaggtagtt gcatcttata ttcactcgcc agaacggtgt
     5821 taggcaaacc agccagtgca taatgcgctt ctccgactcc tttctctgca cagagaacca
     5881 gaccgatggg cggattttca tccggtagcg tccagtgctc tttggcgtag ttgagataca
     5941 tattcatctg tccggcatcg ctatagctga atttgcccac ttttagatcg acgattagca
     6001 ggcagcgtaa acggcggtgg aaaaacagca gatcgacacg aaaccagttg tcatcaatgc
     6061 gtaaccttcg ctgccgacca acaaaggcaa aatcatcccc aagttccagc atgaaatcca
     6121 tcaggtggtt gatcagcgcc tcctcaaaat cggattctga gtattcatct ttcaattcca
     6181 gaaattcgag cacaaaggga tcgcgtatcg cctgttgcgg aagaatatgc gtctcggcag
     6241 gagcgtgttg ttgcagcatg gctgatttgt catgtgacag cagtgttcgc tcataaaatt
     6301 gggtcgcaat ttgccgttct agctggcgaa cagaccagcc acagcggagc gtctcttttt
     6361 cataaaagct gcgggtgtca gcgtttttaa cggaaagtaa acggacgtaa gtggaccagg
     6421 gcaatggtgt taattcgcca gacactgtct ggcgaatttc aacatgttga aaaaagaggt
     6481 aaaaaagcct catttggcgt aagttttttg cagagaaccc acgcttatac cttagactta
     6541 aatcctttga tagtcgcttg attagctgcg caccatacgc agccctggcc tcgccacctt
     6601 gttcaaattc gacaattcgt cggccaattt cccagtatgt cgcggtcatc agcgcgttaa
     6661 cgctacgtac cgtttccgtc cgggcgctat cgaccaggtg aataataccg tcgtggattt
     6721 gctggtagcc tgttgaggtt ctctctgaga gcgattccat tatctttccc tttatatggc
     6781 gcagtgttga ataatggaat cagctatacg ccgggcatct gccggtaaca atccagatgt
     6841 gcgtattaca ttggttttct tcagattatg cgaggcgctt tccagaacga aaggagaatt
     6901 tcagcgccga ctcttttctg aggaggcggc gctgggacag agcttaaact tccagccagt
     6961 tcataatgcc gtctgccgcc ttacgacctt cggcaatagc ggtaacgacc agatcggaac
     7021 cacggacgat atcgccgcca gcaaagattt tcgggttgct ggtctggaaa gcgttgtcgc
     7081 tgccttccgg ggcgatgatg cggccttgcg aatccagctc gacgctgtgt tttgccagcc
     7141 attccatgct gtgtggacgg aaaccaaacg ccatgatcac cgcatctgct ggaacgatat
     7201 gttcggaacc tgccacgatc tccgcgcggc gacggccttt ggcatccggt tcgcccattt
     7261 cagtacgcac catttttacg ccgctgactt tgccgttacc gttcacttca attcccagtg
     7321 gctggacatt gaatttgaac tctacacctt cttcccgcgc gtttttcact tcgcggcggg
     7381 aacccggcat gttctcttca tcacgacgat aggcacaggt aacgtgcttt gccccctggc
     7441 gtacagacgt gcgcacgcag tccattgcgg tatcgccacc gccaagtact accacgcgtt
     7501 tgccttccat gctgacgaac ggttcttcac gagtttcgcc aaagcccatt aactgtttgg
     7561 tgttggcgat aaggaacggc agcgctgcgt acacgccatc ggcgtcttcg ttttccagcc
     7621 cgccgcgcat tgactgataa gtcccgacgc caaggaacac ggcatcgtaa tcactcagca
     7681 gatcgtccag ctgtacgtcg cggcccactt cggtattgag tttgaattca atacccatgc
     7741 cggtgaagat ttcacggcgg cgcgtcatta cctctttttc cagtttgaaa gccggaatac
     7801 cgaaggtcag caagccgcca atttccgggt gacggtcgaa gacgaccgct tgtacgccgt
     7861 tgcgcgtcag gacatctgca cacgccagac ctgccggacc tgcgccgata atcgccactt
     7921 ttttaccggt ctgtttcacg ccggacatat ccggacgcca gcccatttcg aacgctttat
     7981 cattgatata acgctcaatg ttgccgatgg tcaccgcgcc aaactcatcg ttcagggtgc
     8041 aggaaccttc gcacagacgg tcttgcgggc agacacgtcc gcatacttcc ggcagagtgt
     8101 tggtctggtg cgacagttct gccgcttcaa aaatacgccc ctcgttggcg agcttcagcc
     8161 agttcgggat gtagttgtgt accgggcatt tccactcgca gtatgggttg ccgcacgaca
     8221 ggcagcggtc agcctgcgct ttggcctggc cttcggaaaa cggctcgtaa atttcaacaa
     8281 actcaatttt gcggatcttc agcggtttct ttggcggatc aacgcgctgc aggtcgataa
     8341 attgataaac attctgactc attgttgcta ccccttactg cgcctgcacg cgcaactctg
     8401 ctgcgctacg actacgatga cccagcagtg ctttgacatc actggacttc ggtttaacca
     8461 gcgcaaattt agtggcgaag gttgaccagt tcgccagaat ctcttcacca cgctgggaac
     8521 cggtatgctg cacatgctcg gtgataagac cacgcagatg ctcttcatgg atcgccaaat
     8581 cgtcaacgct taatacttcc accagttccg ggttaacgcg tttgcggaaa tcgccgcttt
     8641 catcgagaac gtaagcgaag ccgccggtca tacccgcacc gaagttaacg ccggttttac
     8701 ccagaataca gacgataccg cccgtcatat attcacagcc gttgtcgcca atgccttcta
     8761 ccacggtgat tgcaccggag ttacgcacgc cgaaacgttc acccgcgcgg cctgcggcat
     8821 acagacgacc acccgtcgct ccatacaggc aggtgttgcc gataatactt gcttcatggc
     8881 tgcggaaggc ggaaccaacc ggaggacgaa tggcgattaa gccgcccgcc atgcctttac
     8941 cgacatagtc gttggcatca ccggtcaggt acagttccac gccgcccgcg ttccacacgc
     9001 cgaagctctg gcctgcggtg ccgttgaagt acgctttaat cggatcggct gccagcccct
     9061 gatcgccgtg cgtctgggcg atatagcctg aaagcgacgc gccgacagag cggtcggtgt
     9121 tgcggatatc gaaccagaag gttttgctct ggcgctcatc gacaaacggt ttcgcctgtt
     9181 gcagcaactg cgcgttcagc aggccgttat caaacggcgg gttggtttcg gtgcagtaga
     9241 gtgccttacc tggatgcggt tcggcggtct ccagcagctt cgacagcgcc agtttctgct
     9301 gtttggcggt gaaaccgtcc agctctttca gcaggtcggt gcgaccaatc agatccacca
     9361 gacgtgttac gccaagctgt gccatcagct cgcgggtttc acgggcgata aactcaaagt
     9421 aattcgtcac cttgaacggc aggccgtgat agtgattctt acgcagtttg tcatcctgag
     9481 ttgccacacc cgttgcgcag ttgttcaggt ggcaaatacg cagatattta cagccgagcg
     9541 cgaccattgg gccagtgccg aagccgaagc tttctgcgcc gagaatcgcc gctttgatga
     9601 tatcgacacc cgttttcagg ccgccatcga cctgcaaacg gattttatga cgcaagccgt
     9661 tggcaaccag cgcctgttgt gtttcaacaa gtcccagctc ccacggacag cctgcgtatt
     9721 tcaccgatga aagcggactt gcgccggtgc cgccgtcata gcctgcgatg gtgattaaat
     9781 cggcataagc tttcgccaca ccggtggcga tggtgccaac gcccggttcg gaaaccagct
     9841 tcacggagat catcgctttc gggttaacct gcttgaggtc gaaaatgagc tgcgctaagt
     9901 cttcgataga gtagatatcg tggtgcggcg gcggggagat cagcgtcacg cccggcaccg
     9961 aatagcgcag tttggcaatg taaggagtga ctttgtcgcc cggcaactga ccgccttcgc
    10021 ccggcttcgc gccctgggcg actttaatct gaatgacgtc ggcattgacc agatacgccg
    10081 gagtaacccc aaagcgaccg gaagccacct gcttgatgcg cgacactttg ttggtgccgt
    10141 agcgcgccgg gtcttcacca ccttcaccgg agttcgagtt tccgccgatg ctgttcatcg
    10201 cttccgccag cgcctcgtgg gcttccgggc ttaacgcgcc gatagacatc gccgcggtat
    10261 cgaagcgttt aaacagttcg cttgccggtt caacatcggc aatgttgacc gcgttttcac
    10321 ccggcgtaat tgccagcaga tcgcgcagcg tggttgccgg acgctcatta accagcttcg
    10381 cgtattcctg atagtcgcta tactcgccgc tctgtaccgc ctgttgcagc gtgcgcacca
    10441 cgtccgggtt gtaggcgtgg tattcgccgc cgtggacgta tttcagcagg ccgccctggc
    10501 tgatgggctt gcgcgccagc caggctcgtt tcgagagatt cagcagatcc tgctggaagt
    10561 cttcaaagct cgccccccca atgcggctga ccgccccctg gaagcacagg cccactacat
    10621 cattgtgcag accgaccgct tcaaacagtt tcgagcagcg gtaagaggcg atggtggaga
    10681 tgcccatttt ggacatgatt ttgtacaagc ctttgttgat gccgttacgg tagttgagca
    10741 tcacggtacg ataatctttg gcaatcgcgt gggtgtctac caggcggccc agcgtttcat
    10801 aggcaaggta cggataaata gccgtcgcgc cgaaacccag caacacggcg aagtggtgcg
    10861 gatcgcgggc gctggcggtt tcgacgatga tgttggcatc gcaacgcagg ctttgatcga
    10921 ccagacgggt ctggatcgcg ccaaccgcca ttggagccgg aaccggcagg cgatctttag
    10981 cgatattccg gtcggagagc accagcagca cggtgccgct acgtaccatt ttttcggctt
    11041 tgtcgcacag ctctttgacc gtcgcttcga gcgtggtttt ggtgacgtca aaggtgatat
    11101 ccagcgtatc tgcgcggtag tgctcctctt tcatcgtcgt gagctgtttg aaatcggagt
    11161 agagcagaat cggcgattta aagcttaaac ggtgcgcctg gccctctgct tcgcaaaaga
    11221 cgttcatttc acgaccgata ctggtggcga gcgacataac atgcgcttca cgcagcgggt
    11281 cgattggcgg gttagtcacc tgggcaaact gctggcggaa gtagtcgtaa ataatgcgcg
    11341 gctgactgga gagcacggcg aatggggtat catcgcccat cgaaccgacc gcttcctgac
    11401 cgttttcgcc cagtacgcga attacggagt ccagctcttc cgcgctgtag ttaaactgtt
    11461 tctggtagct ggcaagcgtg tcgtcgtcca gttcgcggct acccacttct tcatcgggca
    11521 gatcttcaaa cggtaccagt cggcggacgt ttttctccat ccactcttta taagggtggc
    11581 ggcttttcag atcgtcatcg gtttctgccg agtgcagaat acgcccactg cgagtgtcga
    11641 ttaccatcag ttcgcctggc ccgacgcggc ctttttcgac cacttcgtca ggctggtaat
    11701 cccagatacc gacttcagag gcgcaggtga tgagcttatc tttggtgatg acgtagcgcg
    11761 ccggacgcag accgttacgg tcgaggttac aggcagcaaa acgaccgtca gacatcacga
    11821 tgcccgccgg gccatcccac ggctccatat gcatggagtt aaagtcaaag aaggcacgca
    11881 gttccgggtc catatccggg ttgttctgcc aggcgggtgg tactaataga cgcatggcgc
    11941 ggatgatatc catcccgcct gccagcagca gttccagcat gttatccatc gaactggagt
    12001 cagagccgat ttcgttgacg aacggtgcgg cgtcgtgcag gtcagggata agcggtgtct
    12061 ggaatttata agtacgcgca cgcgcccatt ggcggttacc ggtgatggtg ttgatttcac
    12121 cgttatgcgc caggtagcgg aacggttgcg ccagcggcca gcgcggtacg gtgttagtgg
    12181 agaagcgctg gtggaacagg caaatggccg attccagacg caggtccgca agatctagat
    12241 aaaagcgcgg cagatccgcc ggcatacaca gacctttata gatgttcacc agattcgaca
    12301 ggctacagac gtagaagtct ttgtcggctt cgagacgctt ttcaatgcgg cggcgggcga
    12361 taaacagacg gcgctccata tcgcgcggac gccagcctgc cggggcgttc acaaaaattt
    12421 gctcaatgcg tggcagagag gagagggcga tttcacccag cacgccttcg ttagtgggga
    12481 catcacgcca gcccacaatc gacaaggttt cgcgttgcag ctcttcttca acgatgcggc
    12541 gtgcggcagc ggcgagttca ggatctttat tcaggaagag catcccgacg gcgtagtttt
    12601 tcgctaaacg ccagccgcgc tcctgcgcaa cgatgcgaaa aaagcgatcc ggtttttgta
    12661 acagcaagcc gcaaccgtca ccggttttac catcggcgag aatcgcgcca cggtgctgca
    12721 tgcgggccag tgcgtgtatt gcagtacgca ctaccttgtg gctaggttcg ccttctatgt
    12781 gggcgatcag gccgaaacca cagttatccc tctcaaggga tttatcgtac aacatatcgt
    12841 gaacctcccc caggctctgc gggaacccca cttccgaacc gctgcgcacg ggcacagaaa
    12901 gagcgtggcg acggggtttg cgtgtcatac gcgcgcctcg cattcgcccc cttgttgtcc
    12961 ttttcgcatc ggttaatacg gtgaagaggg gatctcaatt actgcataaa tatgatgagc
    13021 aggctgctca tccagaaagc ttccagcgga tttccaactt atcgggaaag ctgatacagg
    13081 tcaaatggca agcttattgg tacagaaatg tgctttagcg caaaattctt tattaattga
    13141 ctgaaattaa aatggatttt ctaattaagg ctaaaagggg cgtcgttaga gaaacagcca
    13201 attcctgact gtcataaagg cgtgttaacc cttatcctgc cttgaactga cgagaaaacc
    13261 agcattttat actgccttaa ttggtaatga cgtttcatgc tactgttttg cctaaaatcc
    13321 ataaggagat aaaaaaatct gacacaagat aagcaaaagt gatttatgtg attatgcatg
    13381 aaatttcggt aattttgatt ggttatgcag ttgataaaag caggccggga cgattgatcc
    13441 aagtcatcgc cgaattgagc taaaaatggc aggctttggc ctcttttcaa ggcgcggtct
    13501 atcagattat gcagttacag aaattagtca atatgtttgg tggtgatctc acccgtcgtt
    13561 atgggcaaaa ggtgcataag ctgacgctgc acggcggttt tagctgccct aaccgtgacg
    13621 gtaccatcgg gcgtggcggc tgcacattct gtaatgttgc ctcatttgcc gatgaagcgc
    13681 agcaacatcg ttccattgcc gagcaactgg cgcaccaggc gaatttagtt aaccgcgcta
    13741 aacgctatct ggcctacttt caggcgtata ccagcacctt tgcggaagtt caggtgctgc
    13801 gttctatgta tcagcaggcg gtgagccagg ccaatattgt cggtttgtgt gttggtactc
    13861 gcccggactg cgtgccggat gcggtgctgg atctgctttg cgaatataag gaccagggct
    13921 acgaagtgtg gctggagctg gggctacaaa ccgcccacga caaaaccctg catcgcatta
    13981 atcgcggcca tgattttgcc tgttatcagc gtacaaccca gctggcacgt cagcgcgggc
    14041 tgaaggtgtg ttctcactta attgtcggcc tgcctggcga agggcaggct gaatgtttgc
    14101 aaacgctgga gcgagtggtt gaaaccggcg tggacggcat aaagctgcat ccgctgcata
    14161 ttgtgaaagg cagcattatg gcgaaagcct gggaagcggg gcgtttgaac ggtattgaac
    14221 tggaggatta cacgctcact gccggagaaa tgattcgcca tacgccaccg gaagtgatct
    14281 accaccgcat ttccgccagc gcccgtcgcc cgacgctgct tgctcctctg tggtgcgaaa
    14341 atcgatggac gggaatggtc gagctggatc gctatctgaa tgaacatggc gtacagggat
    14401 cggcgctggg acgtccgtgg ctacctccaa cggagtaggt cgttgagggg aattccgcat
    14461 ttctcacaca atttataacg taactgtcag aattgggtat tattggggca ggttgtcgtg
    14521 aaggaattcc ctaatgaagc aaattcgtct gctggcgcag tattatgttg acctgatgat
    14581 gaagttaggt ctggtgcgct tctcaatgtt gctggcgctg gccctcgtcg ttcttgccat
    14641 tgtggtacaa atggcggtaa ccatggtgct gcatggtcag gtcgaaagca ttgatgttat
    14701 tcgttctatc ttctttggtt tgctgattac gccgtgggcg gtctactttc tatcggtggt
    14761 cgtcgagcaa ctggaggagt cacgacaacg tctgtcacgg ctggtgcaaa aactggagga
    14821 gatgcgcgag cgcgatttga gcctcaacgt tcagttaaaa gataatattg cccagctaaa
    14881 tcaggaaatt gccgttcgtg aaaaagcgga agcagaactg caggaaacct tcggccaact
    14941 gaaaattgaa atcaaagagc gcgaagagac acaaattcag ctcgagcagc aatcctcatt
    15001 tttacgttcc ttccttgatg cttcacccga cctggttttt tatcgtaacg aagataaaga
    15061 gttttccggc tgtaaccgcg cgatggagct gctgaccgga aaaagcgaaa aacaactggt
    15121 tcacctgaaa cctgctgatg tttactcacc ggaagccgct gcgaaagtca ttgaaaccga
    15181 tgaaaaagtg ttccgtcata atgtgtcact gacctatgag cagtggctgg attatcccga
    15241 cggacgcaaa gcctgctttg aaattcgtaa agtgccgtac tacgaccgcg tgggtaaacg
    15301 tcacggtttg atgggctttg gtcgcgacat taccgagcgt aagcggtatc aggatgcgct
    15361 tgaacgcgcc agccgcgaca aaacgacgtt tatttccacc atcagccacg aattgcgtac
    15421 accgctgaac ggtatcgtcg gcctgagccg cattctgctg gataccgaac tcaccgccga
    15481 gcaggaaaaa tatctcaaga ccatccatgt ttcggccgtc acgctgggga atatctttaa
    15541 cgatattatc gacatggata agatggaacg gcgcaaggtc cagcttgata atcaaccggt
    15601 tgatttcacc agcttccttg ccgatctgga aaatctctcc gcattgcagg cgcaacaaaa
    15661 aggattgcgc tttaacctgg agccgacgct gccattaccg catcaggtca ttaccgacgg
    15721 gacgcgttta cggcagatcc tgtggaacct catcagtaac gccgtcaaat tcacccagca
    15781 aggccaggtt accgtgcgcg tgcgctacga tgaaggcgat atgctgcatt ttgaagtgga
    15841 agattctggt atcggcattc cgcaggatga gctggataaa atcttcgcca tgtattacca
    15901 ggtgaaagac agtcatggcg gtaaacctgc caccggcacc ggtattggtc tggccgtttc
    15961 tcgtcgtctg gcgaaaaata tgggcggcga tattacggtt accagcgaac agggtaaagg
    16021 ttcaaccttt acgttgacga tccacgcacc gtcggtagca gaagaggtcg atgatgcgtt
    16081 tgatgaagac gatatgcctt taccggcgct gaatgtgctg ctggtggaag acattgaact
    16141 gaacgtgatt gtcgcgcgtt ctgtgctgga aaaattaggt aacagcgttg atgtcgccat
    16201 gaccggcaag gcggcgctgg agatgtttaa accgggcgaa tacgacctgg tgttgctgga
    16261 tattcagttg ccagatatga ccgggctgga tatctctcgt gaactgacga agcgttatcc
    16321 gcgagaggat ttaccgccgc tggtggcctt aaccgctaac gtgctgaaag acaaacaaga
    16381 gtacctcaat gctggaatgg atgatgtgct gagtaagccg ctttctgttc cggcgctaac
    16441 cgcgatgatc aagaaattct gggataccca ggatgatgag gagagtacgg tgacgacaga
    16501 agagaacagt aaatcagaag cattgctcga tattcccatg ctggaacagt atctcgaact
    16561 tgtaggaccg aagctgatca ccgacgggtt agcggtgttt gagaagatga tgccaggcta
    16621 tgtcaacgtg ctggagtcga atctgacggc gcaggataaa aaaggcattg ttgaggaagg
    16681 acataaaatt aaaggtgcgg cggggtcagt ggggttgcgc catctgcaac agctgggtca
    16741 gcaaattcag tctcctgacc ttcccgcctg ggaagataac gtcggtgaat ggattgaaga
    16801 gatgaaagaa gagtggcgtc acgacgtaga agtgctgaaa gcgtgggtgg caaaagccac
    16861 taaaaaatga ccccggctag accggggtgc gcgaatactg cgccaacacc agggaaatct
    16921 tggctgcgcc gtaaattatt atgatgagtt acaagggcac tgcactgttt ttcaggccgc
    16981 acgcagttaa gatagcaaaa cttaaatgat ttgttacatg aatcagttaa atgtgtgatg
    17041 cgtaccattt ttattaaaaa aattaatgtg tttcagtcaa ttgcgaaagg atgatcataa
    17101 tgaagaaaat tggcgtaatt ctgagcggat gcggcgtcta tgacggttct gaaattcatg
    17161 aagcggtgtt gacgttgtta gctatttcac gcagcggtgc gcaggcggtc tgctttgcac
    17221 cggataagca gcaggttgat gttatcaatc atttaactgg cgaagcgatg acggaaacgc
    17281 gcaatgtgct gattgaagcg gcacgaataa cgcgcggtga aatccgtcct ctggcccagg
    17341 ccgatgccgc tgaactggat gcgttgattg tgccgggggg gtttggcgcg gcaaagaatt
    17401 taagcaattt tgccagtctt ggtcgcgaat gcaccgttga ccgtgaatta aaggcgctgg
    17461 cacaagcgat gcatcaggcc ggaaaaccgc ttggttttat gtgtattgcc ccggcgatgc
    17521 tgccgaaaat tttcgatttc ccgctgcgtt tgaccatcgg tactgatatc gataccgcag
    17581 aagtgctgga agagatgggc gcggagcatg tgccgtgtcc tgtcgatgat atcgtggttg
    17641 atgaagacaa taagattgtc accaccccgg catatatgct ggcgcagaac attgcagaag
    17701 cggcgagcgg cattgataag ctggtttccc gcgtgctggt tctggctgaa tgagtaaaag
    17761 ccgcttaacg gtgtttagtt tcgttcgccg ttttcttctg cggttaatgg ttgtcctcgc
    17821 cgttttctgg ggcgggggca tcgcgttgtt tagcgttgca cctgttccct tctctgcggt
    17881 aatggtcgag cgacaggtca gcgcctggct gcatggtaat tttcgttacg tggcacattc
    17941 tgacagggtc agtatggatc aaatctcgcc gtggatggga ctggcggtga ttgccgcaga
    18001 agatcagaca tttcctgagc actggggctt tgatgtcgct tccattgaga aagccctggc
    18061 gcacaatgag cgcaatgaaa accgtattcg cggtgcttca acgatttctc aacagacagc
    18121 caaaaatctc tttttatggg atgggcgtag ctgggttcga aaagggctgg aagccggatt
    18181 aacgctgggg atagaaactg tctggagcaa aaagcgtatc ctgacggttt acctgaatat
    18241 cgccgaattt ggcgacggtg tgtttggcgt cgaagctgcg gcacaacgtt atttccacaa
    18301 acccgcgagc aaacttaccc gatcggaagc tgccttactg gcagctgtat tacctaatcc
    18361 acttcgtttc aaagtctccg cgccatcggg ctacgtgcgc agccgtcagg cgtggatttt
    18421 acggcagatg tatcagttag gcggtgagcc gtttatgcaa caacaccagc tggattaagc
    18481 ttcgcgcgaa gcgagggcca gcgttttgct ctcacgaggt tgcagatctt tctgcagggc
    18541 aatagccagc gccggttggg cgataaatcg tttccacaac cgctcttgct tacgcagaca
    18601 acaccacttt gaccaggtcg ccagtggaat caatgtgctt tcaccaatgt tgtcgcagac
    18661 gacggggata acctctgact cactaatgcg gtgacagagg atattctgcg gcttcagcga
    18721 catcgtcacg atacggttat cctgcaaata acgcttcagc tgtttcagta attggcgcag
    18781 ttgtgcaacg tcttcttcgt aacggcattg ttcagcaaat tcggtcagcg taatggaggg
    18841 cttgccgtca aaatcagcaa tcacatcata aacataaccg gttccgcaat ccgtctcgac
    18901 ggtaccgtga tagcgcggta ttccactcca gtcttttaac cggcgaccaa gatgcgcgta
    18961 gtactttaac tcacgacgga tctctttatc gccaccatcg ccacgatggt agacaatctt
    19021 gatacagcgt tgggcatctt ccggatgcgc ataacatttc cgatgtcttc cggtgcccag
    19081 gggactttgt tcagataagc gaatcatgcc atctcctgga aatattttct gacgatctcc
    19141 agtatggtcg attaacttaa acgaaaccta aacaatgctt cttaaggttt tcttaaggtt
    19201 cttctgaaag taaaaagcgc acgaaatgta caattgacgt tgattgagaa cgatttcagt
    19261 acgtgatttt cttattgtcg gggggagttt gaagggagtt gtatgtcaaa gtgatgaaga
    19321 ttaatcttca tcaaaaccag aattaaagag ggcgataacg gcggccagtg cttcctcttc
    19381 ctgtggaccg gtcgcttcaa cttcaatctg ccgtcctttg gcagaatcca acatcagcag
    19441 cgcaataacg ctgttggctt cagcctcggt gccttcgtca ttacgtaaca gcacttcagc
    19501 gtcaaaaccc tgcattaatt caaacagctt cattgcaggc cgggcatgca tgcccagctt
    19561 gtttgtgatt tcaacagttt gcttgacagt catggtttac gtttttccag cgtacgatgg
    19621 cgtgactgga cgtttttacc gcgcgagcgg aagtagtctg ccagttgctc tgcaatatac
    19681 accgaacggt gcttcccgcc ggtacaacca atggcgaccg tcaagtagct acggttgttg
    19741 gtttccagca taggtaacca tagctcaaga tagcttcgcg tctggtagat aaaattgtgt
    19801 acttctgtgt ggcggtcgag gaacgcggcg acaggcttat caagacctgt cattggacgc
    19861 agtttcggat cccagtgcgg gttcggcaag aagcgcacgt caaagacgta atctgcatcg
    19921 atagggatac cgtgtttgaa gccgaaagac tcaaagacca ttgtcagttc gcgttcacgt
    19981 ttacccagca gacgggtacg cagcatttct gccagctcgt gaacggacat ttctgaggta
    20041 tcgacaatca gatccgctcg cgaacgcaga ggctccagca gatcgctttc tttgtcgata
    20101 gcactttcca gcgacaggtt tttgctggaa agcggatgca gtcggcgcgt gtcactgtaa
    20161 cgacgaatta aggtattacg gtcggcatcc aggaacagta gttgcggtga gaaagcgtca
    20221 ggcaggttac tcatcgcctg ttcgaatatt tctggtgact ccggcatatt acgaacatca
    20281 atgctgacgg cggcagaaat ctcgcgatcg gccagggttc gagccagatc gggtaacaac
    20341 actacgggaa ggttatccac gcagtaaaaa cccatatctt ccagcgcacg cagggcgaca
    20401 gatttacctg aacctgaacg tccgctgacg atcatcagta ccatgtactg tttctcctca
    20461 caacgtctaa aagagacatt accgaataac tacgcttcat ccggagtacc tttggtatcc
    20521 gtaatgattt gatacagctc ttcatcgctc tgggctgcgc gtaaacggcg gcagatggtt
    20581 ttgtccgcca gacgtttcgc caccagcgac agagtatgca ggtgcgtttt agtttggtct
    20641 gccggcacca gcagggcaaa aagtaagtcc accggttggt tgtcgatggc atcgaaagct
    20701 ataggcgttt cgagctgaac gaaaacgcca acggcacgca gagtatcttc ttccagtttg
    20761 ccatgcggaa tggcaatacc attgccgata ccggtactgc ccattttttc ccgcgtcagg
    20821 atagcttcaa aaaccacctg aggcggtagg ctaagttgtt tcgccgccag ctcgctgatg
    20881 atttccaggg cgcgtttttt gctctgacag tggacgcggc ttcgcgtaca ttccctgtta
    20941 agaacactgc taagctgtag agttgtatca ttatttgtca taatttcacc taagaacctg
    21001 cccactcaaa cagtggaaca ggctcggagc attaaccgta caaatggccc gttgtgccgc
    21061 acaacaggcc gccatgcaca tgctaattgc ccggacaatt agtgttgttt cagtttatct
    21121 ttatgtttgg tcaactggcg tgccagctta tcaattaagc catcaatggc agcgtacatg
    21181 tcctgacctt ccgcgctggc atgaatttca ccaccgttta catgcagtgt tgcatctgag
    21241 gtgtgggtga ctttctccac tttcagaaca acatagacct ggttgattcg gtcaaaatat
    21301 tgctcaagtt tggcaaattt ggctgtaaca aattcgcgca gtgcctcggt gatctcgacg
    21361 ttatttccgg taatgttgag ctgcatagtg tcttccttat cggttgggtc aaacgagctg
    21421 tttacgctgg tttgacggcg gaatggataa agactctcgg tacttcgcaa cagtgcggcg
    21481 tgccaccatg ataccttgtt ccgacagcaa agaggttaac ttgctgtcgc tcaacggttt
    21541 cgctgggttt tccgccgcga ttaatttctt caccagcgca cgaatcgccg tggaggaagc
    21601 ttcgccgccg ccctcggtat tgacgtgact ggaaaagaaa tacttcagtt caaaaatgcc
    21661 tcgtggacta tgcaggtatt tttgcgtggt cacgcgagat atcgtcgatt catgcatttc
    21721 gacagcctgg gcgatatcgg ccagtaccat cggtttcata tattcttcgc cttgctcaaa
    21781 gaaggcttgc tgctgttcaa cgatacagcg actcacgcgc agtagcgtat cgttacggct
    21841 ttccagactc ttgatcaacc atttggcatc ttgcagattg ctgcggataa actggctgtc
    21901 accatcattg cgcgcgttat tgcacatcga ggcgtagtgc tggttgattt gcagacgagg
    21961 aatgctgtca ctgttgagtt ctaccgtcca gtgaccgtta tgcttacgca ccagcacatc
    22021 tggaatgaca tactcaggtt cgccagtctg gatcgattgc ccggggcgcg gatcgagcga
    22081 ctggatcaga ttgacggctt ctttcagcac atcttctttc agacgcgtga cgcgcattaa
    22141 agtgcggaag tcgtgattgg ctaacagatc gagatgatcg ctaatgatca gtctggcctc
    22201 ttccagccac ggcgtggttt tatcgaattg ggagagttgg atcagcaggc agtcacgcag
    22261 atcttttgcc gccacaccga ccggatcaaa ccgttggatc cgcttaagga cggcttcaac
    22321 ttcgtcgatg tcgatctctt catcgcccat actttcgaga atatcttcca gcgggacagt
    22381 cagataaccg gtgtcatcaa cggcatcgac gatagaggta gcaatagcgc ggtcagtgtc
    22441 ggaaaacggt gtcagctcga cctgccacat caggtaatcc tgcaaggtct gcgtcgtttc
    22501 gccctgatag accggcagct cgtcgtcaat gtagtcaccg ctggtgccgg atggtgtacc
    22561 agcggtgtaa atggtgtccc aactggcatc gagcggcagc tcttccggca tctctttttg
    22621 ttcgagcgcg tcggcggtgt ccagcgtttc actgtcttgc gtttcgcggg tgtcgatttc
    22681 ttcatgagtg tcgatttgct caagcagcgg attactctcc agcgcctgct gtagctcctg
    22741 ctgaagttcc agcgtcgaca actgcaacag acgaattgcc tgttggagct gtggcgtcat
    22801 cgccagttgt tggctaagcc tgagttgcaa accttgcttc atgttcagaa tcgtactctc
    22861 ctgctaaaac gtcgcaaact tctaccctat cagagtctga agtcttcccc aaggtataca
    22921 cgcttaacgt gttcgtcttg taagatttct gtaggcgtgc cgtgggcgat caaatgcccc
    22981 tgactgacga tataagcgcg ttcacaaacc gccagtgttt cacgcacgtt gtggtcagtg
    23041 atcagcacgc ccaggccgct gtcgcgcagg tgctcaatga tgcgtttaat gtcgataact
    23101 gagatcgggt caaccccggc aaacggttcg tcgagcagaa taaatttcgg attcgcagcc
    23161 agtgcgcggg caatttctac acggcgacgt tcacccccgg agagtgactg ccccatgctg
    23221 tcacgcaggt gctcaatgtg aaactcttcc atcagctcgt tcgcgcggtc ttcacgctgt
    23281 tcagcagaca agtcgtcacg aatttgcagt accgccatca ggttatcgta aacgctgagg
    23341 cgacggaaaa tggaggcttc ctgtggcaga tagccgatac cgcggcgcgc gcgtgcatgc
    23401 agaggtagca gactgatatc gtcatcatca ataatgatgt tgcccgcatc gcgcggcaca
    23461 atgcctacaa ccatgtagaa agtggtggtc ttaccggcac cgtttggccc cagcagaccg
    23521 acaatttccc cggagttgac ggtcaggctg acgtcttcta ccacgcgacg gcctttatag
    23581 gcttttgcaa ggttctttgc agttaatgtt gccataacga attaattacc cttcttctgt
    23641 gccggggtct ggcctttgtt gtttttgtcc tgcagctgcg acggcaccag aacggtggtt
    23701 acacgcttgc ctttgtcgct gaaagcctgc attttctgct ctttcaccag gtaagtgatc
    23761 ttatcgccct taatgttgct atcgacctgc tgcagataag cattacccgt cagaacgaca
    23821 aaatcttttg ccagttcgta gtgcatctgg gaagcgtgac cttcaacggg tttaccgttg
    23881 tcctgcatct ggtagaacgt tgccggttta ccgtagccgt caatcacttc tttaccttgt
    23941 tcgccacccg gacgggtaac gaccactttg tcggcattaa tttttatggt gccctgggtg
    24001 acgatgacat taccggtaaa ggtaaccacg ttgccttgca tatcaagaga ttgctggtcc
    24061 gattcaatgt gtatcggctg atcagtgtct ccggttacgg caaatgccgg aatgctggcg
    24121 gccagaagtg agctggcaag cacaagatta aggctgagtt tgtttgtttt gaatttcata
    24181 ggatgttcta accttttcaa tcagctcggc gttcttgctg cgtaagttgc cgcgcatttt
    24241 cagaccgctg gagttaaatg ttgttccgta taacgtgacg aggtcttcag aggtaacatc
    24301 ctgcgtcacc agattgatct gcgcgttatc cgtcgtgatt ctgcgaagtt gagagtccgg
    24361 cacaagtgcg ttgacttcaa cgtgtccata taaatagagc atccggtcat tggtcagctt
    24421 ggctttatct gcttttacgg accatgtcgg gattttatcc ttatcaaacg tggtaagtac
    24481 cggctgcgta aaccacgaaa cggcctgatc ggaataatat tcaacgtgtt gagcaatcaa
    24541 tcgatagctt agcgcccctt ctgggttata gacgagcgtg tccgtatgct cgcttttata
    24601 ggtgggatca ttgttgttga cgaccacctg ggcggtatcg tctttttcgg ccatattaat
    24661 accgatcatc accagaaccg ccagtgatag cacaatgata acccaacgtc tggctttact
    24721 catatcgatt gccctttggc ttcatccagt ttgccctgcg ccaggagtaa taagtcgcaa
    24781 acttctcgca ctgcgccacg accgccagca atgcgcgtca cgtaatcggc gcgcgggatc
    24841 aacagtggat gcgcatcggc cacggcgacg cttaaaccca ctttttccat taccggccaa
    24901 tcgatgagat catcgccgac ataagccaca ttttccgggg caatcgccaa tttttccagc
    24961 agatcgctaa aggcgatcag tttgtttgac tgcccctgat acaagtgagt gatccccaat
    25021 gtggcacaac gatcttctac cagtttagcc tttcgcccgg taatgatagc gacttcaata
    25081 tcagaggtga gcgcacaacg aatgccataa ccgtcacgaa cattgaacgc tttcagctct
    25141 tcgccgttat tgcccatata aatcaggcca tctgacagta cgccatcaac gtcgaggatt
    25201 agcagacgaa tgttctctgc ttttgctata acgtcggcgc tgacagggcc gtaacaggtc
    25261 gcaagcgacg cacctgcttt gctcattgtt gtttatcctt gaatctttac actacgcctg
    25321 cacgcagtaa atcatgcata tgtaacacac cgagtaaatg gtcgccatcg gcaaccatca
    25381 cggaggtgat atggcgggac tgcattaagt tcagtgcctc aacggccaga atgccagggc
    25441 gcacgcgtat tccccccggc gtcatcacat cggcaatact taactgacga acatccacgc
    25501 ccatatcgaa gacacggcgt aaatcaccgt cggtaaagat gccttcaatc atcatattgt
    25561 catcgcaaat gacagtcata ccaagatttt tgcgggtaac ttccagcaat gcgtcacgca
    25621 gactggccgt tttcttaaca tgcgggatct catcgcccgt atgcataata tcgtttacgc
    25681 gcagcagaag tttacgaccc agtgcgccgc ctgggtgtga gagcgcaaaa tcttcagcag
    25741 taaagccgcg tgcttttaac agcgcgacag cgagggcatc gcccataacc agcgtggcgg
    25801 tggtgctgct ggtcggtgcc agccctaacg gacaggcttc tttcgctact ttaacacaca
    25861 gatgcacatc tgcggcgcgc gccatgctgc tctccgggcg accggtgatg cagattaacg
    25921 gtatgtgaag acgcttaagc actggaatta aggcagtgat ttcgctggat tcacctgagt
    25981 tagagatagc aatcaccaca tcctgtgggg tgaccatgcc taaatcacca tgcgcggctt
    26041 caccaggatg gacgaaaaat gaaggtgtac cggtgctggc aaacgttgcg gccattttgc
    26101 gcccgatgtg ccccgatttc cccatcccca tgacgacaac tttcccttta caccagaaca
    26161 tcttttcaca ggcaagcgtg aaattctgat tgatgtattg atcaagctcc gccaggcatt
    26221 cacgttcaat cgccaggact tctttacctg cttgctgaaa gtcaaaaccc ggttgtaact
    26281 ctacgtgcga cataatgcgt ttccagttat tcaacgagta ttggcgataa ccagtacaac
    26341 atcgccagcc atacgataaa tccgccagtt aataataccc ctacaccacg gcccggttgc
    26401 ggggagcgcc gccagcacag caacgcaaaa ataatgctca ccagcaacat cacgctgtag
    26461 tcacgactgt acgccagtgg atcaatctct cctggcgtta tcagcgcggg taaacccaac
    26521 acgatgacaa tattaaaaat gtttgcgcca atgatatttc cgacagcaat gtcgttttca
    26581 cctttgcgaa cgcccgctat tgcggttgcc agttccggca ggctggttcc gatagcaatt
    26641 gccgtcagac ccatcgtcaa ctcgctgatg gcaaagtaat tcgccagcac cgtggcgtta
    26701 tcaaccacca tccgcgtggc cactggcatg atgataagcg caatgccgag ccataaaaac
    26761 gcgacgggca atccgccgtc acgcggcagc tctgcaagct gctctctggt caggctgtca
    26821 gtcccctgac gttcagcctg acgtgcaagt ttaacaatga acagcagcca tagcacagcc
    26881 agaaagagga gaaagatacc atcgctgcga ctaagttgtc cgtcatagag tacggaaccg
    26941 gccaccacgc tgaccaacaa cattaagggt aattcacggc gtagaacatc ggaatggacg
    27001 gtaaaaggac gaaccagcgc agccagaccg aggatcagca atatattgat aatgtttgag
    27061 ccgagggctg taccaacggc taaatcgcgt tgttcgtgca gagacgcggc aagcgagacg
    27121 atgatttctg gtaacgatgt accaatactg accaccgtca tgccgatgat cagcggcggg
    27181 atgccaaagg ttcggcaaag aatagacgcg gcaaaaacca ggcggtcggc actgtaaacg
    27241 accaaaagta aaccaacaat taacagtgcc gtagctaaaa gcatctaacg tcctttcttc
    27301 aggtatactc gccggtccgc tgaagatttt cagaaagccg taacggattc ttaattttga
    27361 ctttatgcgg ctaaaaagta aaacaaatgc cagctttcgc taaccacggc gggtaatatt
    27421 ctgtaaatat gttgggttca aggttaaatt gagcgccatg cttagaaaat caacgcaaga
    27481 cgaagggtga attatggagc agtctgtggc gaatttagtc gatatgcgcg atgtcagttt
    27541 tacgcgtggc aatcgctgca tcttcgataa tatttccctg accgtgccgc gagggaagat
    27601 cacggcgatc atggggccat cgggcatcgg taaaacgacg ctactccgtc tgattggcgg
    27661 gcaaatcgca ccagatcatg gtgagatcct tttcgatggt gagaatattc cggcgatgtc
    27721 tcgttcgcgc ctgtatacag tgcgcaaacg gatgagcatg ttatttcagt ccggggcgtt
    27781 gttcactgat atgaacgtat ttgacaacgt cgcctatcca ctgcgcgaac atacccaact
    27841 tcccgcgcca ttgttgcata gtacggtgat gatgaagctg gaggccgtgg ggctgcgtgg
    27901 agcggctaaa ctaatgcctt ctgaactttc cggtgggatg gcgcggcgtg cagcgctggc
    27961 gcgtgcgatt gcgctggagc cggatctcat catgtttgat gaaccttttg ttgggcaaga
    28021 tcccatcacc atgggcgtac tggtgaagct gatttctgag ctgaacagcg cgctgggcgt
    28081 gacttgtgtg gtggtttctc acgatgtgcc ggaagtgtta agtattgcgg atcacgcctg
    28141 gatcctggcg gacaaaaaaa ttgtcgctca tggcagtgcc caggcgttgc aggcgaatcc
    28201 tgatccgcgc gtacgtcagt ttctggacgg gatagctgac gggccggttc cgttccgcta
    28261 tcctgccggc gattatcacg ctgatctttt accagggagt taagccactc atgctgttaa
    28321 atgcgctggc gtcgctcgga cataaaggga ttaaaaccct gagaacgttt gggcgggccg
    28381 ggttaatgtt attcaatgcg ctggtcggca aaccggaatt tcgcaaacat gcgccgctgc
    28441 tggtgcgcca gctctataat gtcggcgtcc tgtcgatgct gattattgta gtttctggcg
    28501 tgttcatcgg aatggtgttg gggctgcaag gttatctggt tctgaccact tatagtgcgg
    28561 aaaccagtct gggtatgctg gtggcgttat cgctactgcg tgaactgggg ccggtggttg
    28621 ccgcgttgtt gtttgccggg cgtgctggtt cggcgctaac cgcagaaatc ggcctgatgc
    28681 gcgctacaga gcaactctcc agtatggaga tgatggcggt ggatccgctg cgtcgggtta
    28741 tttctccccg tttctgggct ggggttattt cattaccact gttgacggtt atcttcgtcg
    28801 ccgtggggat ctggggcgga tcgctggtcg gcgtcagttg gaaaggcatt gatagcgggt
    28861 tcttctggtc ggcaatgcaa aatgccgtcg actggcgtat ggatctggtc aactgtctga
    28921 ttaagagcgt ggtgttcgcc atcacggtga cgtggatttc gttgtttaac ggctacgacg
    28981 ccatcccgac gtctgccggg attagccggg caaccactcg caccgttgtc cactcgtctc
    29041 tggctgttct ggggctggat tttgtgctga ccgcattgat gtttgggaat tgagttcatg
    29101 caaacgaaaa aaaatgaaat ttgggtgggt atctttttat tagcagcact gctggcggcg
    29161 ctgtttgttt gcctgaaggc ggcgaacgtg acgtcaatac gtactgaacc gacctacacg
    29221 ctttatgcga cgttcgataa cattggcggc ctgaaagcgc gctctccggt cagtattggt
    29281 ggcgttgttg tgggccgggt ggcggatatt acgctggacc cgaaaaccta tctgccgcgc
    29341 gtaacgctgg aaattgaaca acgttataac catattcctg ataccagttc gctgagcatt
    29401 cgtacttccg gcctgctggg ggaacaatat ctggcattaa acgtcggttt tgaagacccg
    29461 gaactgggga ctgctatcct gaaggatggc gatacaattc aggacaccaa gtctgcgatg
    29521 gtgctggaag atctcattgg tcagttcctt tacggtagta aaggcgatga caataagaat
    29581 agtggcgatg cgccagctgc tgcgccaggt aataatgaaa ccactgaacc tgtgggtaca
    29641 acgaaataat ttcaggagaa ccgacgcatg tttaaacgtt taatgatggt cgctttgctg
    29701 gtgattgcac cgctgagtgc ggcaaccgcg gcagaccaga ccaatccgta taagctgatg
    29761 gacgaggcgg cgcagaaaac gttcgatcgc ctgaagaatg agcaaccgca aattcgggcc
    29821 aacccggatt atctgcgtac cattgttgat caggaactgc tgccatacgt acaggtgaaa
    29881 tacgccggtg cgctggtgct gggccagtat tacaagagtg cgaccccagc tcaacgtgat
    29941 gcttactttg ccgctttccg tgagtacctg aagcaggctt acggtcaggc gctggcgatg
    30001 tatcacggtc aaacctatca gattgcgcca gaacagccgc tgggtgataa aaccattgtg
    30061 cctattcgcg ttaccattat tgacccgaat ggccgcccgc cggtgcgtct ggacttccag
    30121 tggcgtaaaa actcccagac gggcaactgg caggcttacg acatgattgc tgaaggcgtc
    30181 agtatgatca ccaccaaaca aaacgagtgg ggaacgctgc tacgtaccaa aggtatcgac
    30241 ggcctgactg cgcaactgaa atcgatttct caacagaaaa tcactctgga agagaaaaaa
    30301 taatgagcga gtcactgagc tggatgcaga cgggtgacac actggcgtta tccggagagc
    30361 tggatcagga cgttctgcta ccgctttggg aaatgcgtga ggaagcggtg aaggggatta
    30421 cctgcatcga tcttagccgt gtgtcccgcg tggatacggg tggactggca ctgctgctcc
    30481 atcttatcga tctggcgaaa aagcagggca acaacgtgac gcttcagggg gtaaacgaca
    30541 aagtgtatac cctggcgaaa ttgtataatt tgcctgctga tgttctgcct cgttaaattt
    30601 tttcaggccg gatatggcgt taaccgcctg tccggcaaac ctgcctgcaa gtcgagcctt
    30661 gtctgattta tcagcgaggc tttttgctta tttatgccag cgcaactttg ctctaagatg
    30721 tttcgctggt ttattaactg atgattgaag atcccatgga aaataatgaa attcagagcg
    30781 tgttgatgaa cgctctctcc ctccaggaag tccacgtttc cggcgatggc agccactttc
    30841 aggttattgc cgtgggtgag ttgtttgacg gcatgagtcg ggttaaaaaa cagcagacgg
    30901 tctatggtcc gctgatggaa tatattgcgg ataaccgcat tcatgctgtg tcgatcaaag
    30961 cgtatacccc tgcggagtgg gcgcgcgatc gcaaactgaa cggcttttga gctatgggcg
    31021 attcgccggt agcggatatg aattgttaac tgagaacaaa ctaaatggat aaatttcgtg
    31081 ttcaggggcc aacgaagctc cagggcgaag tcacaatttc cggcgctaaa aatgctgctc
    31141 tgcctatcct ttttgccgct ctactggcgg aagaaccggt agagatccag aacgtcccga
    31201 aactgaaaga cgtcgataca tcaatgaagc tgctaagcca gctgggtgcg aaagtagaac
    31261 gtaatggttc tgtgcatatt gatgcccgcg acgttaatgt attctgcgca ccttacgatc
    31321 tggttaaaac catgcgtgct tctatctggg cactggggcc gctggtagcg cgctttggtc
    31381 aggggcaagt ttcactacct ggcggttgta cgatcggtgc gcgtccggtt gatctacaca
    31441 tttctggcct cgaacaatta ggcgcgacca tcaaactgga agaaggttac gttaaagctt
    31501 ccgtcgatgg tcgtctgaaa ggcgcacata tcgtgatgga taaagtcagc gttggcgcaa
    31561 cggtgaccat catgtgtgct gcaaccctgg cggaaggcac cacgattatt gaaaacgcag
    31621 cgcgtgaacc ggaaatcgtc gataccgcga acttcctgat tacgctgggt gcgaaaatta
    31681 gcggtcaggg caccgatcgt atcgtcatcg aaggtgtgga acgtttaggc ggcggtgtct
    31741 atcgcgtgct accggatcgt atcgaaaccg gtactttcct ggtggcggcg gcgatttctc
    31801 gcggcaaaat tatctgccgt aacacgcagc cagatactct cgacgccgtg ctggcgaaac
    31861 tgcgtgacgc tggagcggac atcgaggtcg gcgaagactg gattagcctg gatatgcatg
    31921 gcaaacgtcc gaaggctgtt aacgtacgta ccgcgccgca tccggcattc ccgaccgata
    31981 tgcaggccca gttcacgctg ttgaacctgg tggcagaagg gactggattc atcaccgaaa
    32041 cggtctttga aaaccgcttt atgcatgtgc cagagctgag ccgtatgggc gcgcacgccg
    32101 aaatcgaaag caataccgtt atttgtcacg gtgttgaaaa actttctggc gcacaggtta
    32161 tggcaaccga tctgcgtgca tcagcaagcc tggtgctggc tggctgtatt gcggaaggga
    32221 cgacggtggt tgatcgtatt tatcacatcg atcgtggata cgaacgcatt gaagacaaac
    32281 tgcgcgcttt aggtgcaaat attgagcgtg tgaaaggcga ataatcgtct gagagctggc
    32341 agccccgttc gcggggctac cgccagacca ctcatttttt cggtttagtg tagctacgca
    32401 tcaactgcgt tctgtcgata aactcatggg tctgcggatc atggtagcgt gatggccaga
    32461 taacccaggg gtcagttccc agggctttcg caataatcat ctctcctttc ggccatgggc
    32521 gcgataatgc attcgccagc gttgaggaac tcaaaccatt tctgcgagac tccgccgcca
    32581 ttgatgttcc cttcttgcgc aaacccgcaa tgatgtcagc gggatgccag tcaatgaaat
    32641 tactttccat ctttcctcac tccttaagtt agtcgctttc attcagaaca cttcttctgt
    32701 cgagcatcac tatactcctg atggccgatt gctctaaaaa gttcctat
//
LOCUS       DBFIFI010000031        32380 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.031, whole genome shotgun sequence.
ACCESSION   DBFIFI010000031 DBFIFI010000000
VERSION     DBFIFI010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32380
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.031"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(106..3255)
                     /gene="acrB"
                     /locus_tag="N7B39_001426"
     CDS             complement(106..3255)
                     /gene="acrB"
                     /locus_tag="N7B39_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HGU2421434.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSIEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHNHTVDHH"
     gene            complement(3278..4471)
                     /gene="acrA"
                     /locus_tag="N7B39_001427"
     CDS             complement(3278..4471)
                     /gene="acrA"
                     /locus_tag="N7B39_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HGU2421435.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            4613..5260
                     /gene="acrR"
                     /locus_tag="N7B39_001428"
     CDS             4613..5260
                     /gene="acrR"
                     /locus_tag="N7B39_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HGU2421436.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            5388..8750
                     /gene="mscK"
                     /locus_tag="N7B39_001429"
     CDS             5388..8750
                     /gene="mscK"
                     /locus_tag="N7B39_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HGU2421437.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRVKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(8789..8953)
                     /gene="rsmS"
                     /locus_tag="N7B39_001430"
     CDS             complement(8789..8953)
                     /gene="rsmS"
                     /locus_tag="N7B39_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HGU2421438.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LQSDEASQSE"
     gene            complement(8967..9494)
                     /gene="priC"
                     /locus_tag="N7B39_001431"
     CDS             complement(8967..9494)
                     /gene="priC"
                     /locus_tag="N7B39_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HGU2421439.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFNRHLFQTRAT
                     TLQACLDKAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREATAWSLREWDSAP
                     PQIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            9564..9941
                     /gene="ybaN"
                     /locus_tag="N7B39_001432"
     CDS             9564..9941
                     /gene="ybaN"
                     /locus_tag="N7B39_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HGU2421440.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     CFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            10094..10645
                     /gene="apt"
                     /locus_tag="N7B39_001433"
     CDS             10094..10645
                     /gene="apt"
                     /locus_tag="N7B39_001433"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HGU2421441.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGINKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            10774..12705
                     /gene="dnaX"
                     /locus_tag="N7B39_001434"
     CDS             10774..12705
                     /gene="dnaX"
                     /locus_tag="N7B39_001434"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HGU2421442.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDAWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEDSIRPI"
     misc_feature    12038..12102
                     /gene="dnaX"
                     /locus_tag="N7B39_001434"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            12758..13087
                     /gene="ybaB"
                     /locus_tag="N7B39_001435"
     CDS             12758..13087
                     /gene="ybaB"
                     /locus_tag="N7B39_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HGU2421443.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            13087..13692
                     /gene="recR"
                     /locus_tag="N7B39_001436"
     CDS             13087..13692
                     /gene="recR"
                     /locus_tag="N7B39_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HGU2421444.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            13802..15676
                     /gene="htpG"
                     /locus_tag="N7B39_001437"
     CDS             13802..15676
                     /gene="htpG"
                     /locus_tag="N7B39_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HGU2421445.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            15857..16501
                     /gene="adk"
                     /locus_tag="N7B39_001438"
     CDS             15857..16501
                     /gene="adk"
                     /locus_tag="N7B39_001438"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HGU2421446.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            16633..17595
                     /gene="hemH"
                     /locus_tag="N7B39_001439"
     CDS             16633..17595
                     /gene="hemH"
                     /locus_tag="N7B39_001439"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HGU2421447.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(17592..18551)
                     /gene="aes"
                     /locus_tag="N7B39_001440"
     CDS             complement(17592..18551)
                     /gene="aes"
                     /locus_tag="N7B39_001440"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HGU2421448.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPPWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATKAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQVIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKVAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            18703..20007
                     /gene="gsk"
                     /locus_tag="N7B39_001441"
     CDS             18703..20007
                     /gene="gsk"
                     /locus_tag="N7B39_001441"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HGU2421449.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(20140..21816)
                     /gene="ybaL"
                     /locus_tag="N7B39_001442"
     CDS             complement(20140..21816)
                     /gene="ybaL"
                     /locus_tag="N7B39_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HGU2421450.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(22053..23273)
                     /gene="fsr"
                     /locus_tag="N7B39_001443"
     CDS             complement(22053..23273)
                     /gene="fsr"
                     /locus_tag="N7B39_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HGU2421451.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            23491..25143
                     /gene="ushA"
                     /locus_tag="N7B39_001444"
     CDS             23491..25143
                     /gene="ushA"
                     /locus_tag="N7B39_001444"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HGU2421452.1"
                     /translation="MKLLQRGVALALLTTFTLASEAALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKLGNPENFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGDHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(25180..25659)
                     /gene="ybaK"
                     /locus_tag="N7B39_001445"
     CDS             complement(25180..25659)
                     /gene="ybaK"
                     /locus_tag="N7B39_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HGU2421453.1"
                     /translation="MTPAVKSLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(25863..26657)
                     /gene="ybaP"
                     /locus_tag="N7B39_001446"
     CDS             complement(25863..26657)
                     /gene="ybaP"
                     /locus_tag="N7B39_001446"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HGU2421454.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDISLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQKLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            26795..27136
                     /gene="ybaQ"
                     /locus_tag="N7B39_001447"
     CDS             26795..27136
                     /gene="ybaQ"
                     /locus_tag="N7B39_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HGU2421455.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(27351..29855)
                     /gene="copA"
                     /locus_tag="N7B39_001448"
     CDS             complement(27351..29855)
                     /gene="copA"
                     /locus_tag="N7B39_001448"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HGU2421456.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADFDEAQALRLAAALEQGSSHPLAR
                     AILDKASDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            30117..31049
                     /gene="glsA"
                     /locus_tag="N7B39_001449"
     CDS             30117..31049
                     /gene="glsA"
                     /locus_tag="N7B39_001449"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HGU2421457.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTSDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGELVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            31052..32344
                     /gene="ybaT"
                     /locus_tag="N7B39_001450"
     CDS             31052..32344
                     /gene="ybaT"
                     /locus_tag="N7B39_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HGU2421458.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
BASE COUNT         7833 a         8564 c         8524 g         7459 t
ORIGIN      
        1 tggttttcgt atgagatcct gagttggtgg ttcaattact ccttaatgtt cgtaggttat
       61 gcataaaaaa ggccgcttac gcggccttag tgattacacg ttgtatcaat gatgatcgac
      121 agtatggttg tgctcgatat cttcattctt gcggctaaag cggcggcgaa ccaccacaaa
      181 gaataccgga acgaagaaga ttgccagtac cgttgcggtc accatcccgc ccattacacc
      241 ggtacctact gcgttctgcg cgccggaacc agcaccagta ctgataacca gcggcataac
      301 gccgaggata aacgccagcg aggtcatcag gatcggacgt aaacgcatcc gcaccgcatc
      361 aagcgtcgct tcaatcagac ctttaccttc tttatccatc aagtctttgg cgaattcgac
      421 gataagtatc gcgttcttcg ccgacaaccc aatggttgtg agcaggccta cctggaagta
      481 aacgtcattg gtcaggccac ggaaggtggc agccagcaac gcaccgataa cccccagcgg
      541 aacgaccagc ataacggaga acggaatcga ccagctctcg tacagcgccg ccagacacag
      601 gaacacgaca atcaacgaaa tcgcgtacag tgaaggtgcc tggttgccgg agagacgttc
      661 ctgataggac atccccgtcc agtcatagcc aacaccggta ggcagtttgc tcgccagttg
      721 ttccatcagc tccattgctt caccggtact tttacccggt gccgcctggc ctaagatttc
      781 catggatggc aggccgttgt aacgttccag acgcggcgaa ccgtactccc aacgagaaga
      841 ggagaacgcc gagaatggca ccatctgacc atcagcagca cgaacatacc agtcgccgat
      901 atcatccggc agcatacggt atttcgcttc tgacatgacg taaactttct tcacacgacc
      961 gcggtcgata aagtcgttca catagctgcc gccccatgca gcgcccagag tggtgttaat
     1021 gtcgttgata gaaacaccca gcgcctgcgc tttttcctgg tcgatatcaa tcttaaactg
     1081 cggggtatct tccagaccgt ttggacgtac gctggtcaac atatcagggt gcttcgctgc
     1141 ttctgcaagc aactggttac gcgcctgagt cagtttttcg tgaccaaggc cagcctggtc
     1201 aatcagctca aagtcaaagc cggttgcggt acccagttcc acgattgcgg gcaggttaaa
     1261 ggcgaaaacc atcgcatctt tgatttgcga gaaagcgcgt gttgcacgca tggtaatcgc
     1321 ttcaactttg ttttcttcac ccggacgatc ggcccagtcc ttcaaggaaa cgaacgcaat
     1381 accggtattc tgaccacgtc ccgcaaagcc gaagccgtta acggcgaaca ccgactcaac
     1441 gttgttcttt tctttggtca gatagtaatg cgttacctca ttgagcactt tctgcgtacg
     1501 ttcctgcgtt gcacctgctg gcagctgaac catggtcata aatacgccct ggtcctcatc
     1561 tggcaagaag gagcttggca gacgcacaaa cagataggcc atgccgacca cgatgatcag
     1621 atacagcacc agataacgcc ccgtactgcg cagaataccg cctacgctgt cggtgtagtg
     1681 gtgcgtgctc ttctcgaaca tgcggttaaa ccagccgaag aagccttttt taccttcccc
     1741 gtgatcgcct ttggcaatcg gtttcagcat ggtggcacaa agagctggag tcaggatcaa
     1801 cgccaccagt accgacagcg ccattgctga aacaatggta atagagaact gacgataaat
     1861 tgccccagta gaaccgccaa agaaggccat cggtacgaat accgccgaca gtaccatcgc
     1921 gataccgacc agagcgccct gaatctgccc catcgactta cgggtggctt cttttggcgg
     1981 caaaccttct tccgccatca cacgctcaac gttttctacc acaacgatag cgtcatccac
     2041 caacaggccg atggcgagca ccatcccgaa cattgttagc gtgtttatcg agaagccaaa
     2101 ggcggcaagg acggcaaagg tcccgagcaa taccaccggt acggcaatgg tcggaatcaa
     2161 cgtcgcgcgg aagttctgca ggaacagata cataaccagg aacacgagga tgatcgcttc
     2221 gaccagcgtt ttaaccactt cgtgaataga gattttcacg aacggcgtgg tgtcgtacgg
     2281 gtaaacaatt ttcagacccg acgggaagaa cggttccatc ttcgccagtt cagcacggat
     2341 tgccgcagcg gtatccagcg cgtttgcacc ggtcgccagc ttgatcccca gaccggaagc
     2401 cggttggccg ttaaactctg cgatgatgtc gtagttctca ccacccagct caatcttcgc
     2461 cacatcacgc agcagtacgc gggaaccatc ctgattcact ttcagcagga ttttgccgaa
     2521 ctcttcagta gaggtcagac gcgtctgagc aataatagag gcgttaagct gttggccttt
     2581 caccggcggt gtaccaccga gctggcccgc cgcaacctgg gcgttctgcg ctttgatggc
     2641 ggtaataaca tcaaccggcg ttagctggaa tttgttcagt tcattcgggt tcatccagat
     2701 acgcatcgcg tactgtgaac cgaacaactg aacgtcaccc acgccagacg tacggctgat
     2761 ggcatctttc atattcgccg ccacgtagtc ggagatatcc tcctgcgtca tggtgccatc
     2821 ggtgttgata acgccgacaa ccatcaggaa gctgctggat gatttctcaa tgctcacccc
     2881 ttgctgctga acttcttgcg gcagcaacgg catcgccagc tgcagtttgt tctgcacctg
     2941 aacctgcgcg atatccgcat cagtaccaga ctcaaaggtc agggtgatct gtacggtacc
     3001 cgtggagtca ctgttagagg acatgtacat caggttatcg ataccgttca tattctgttc
     3061 gataacctgt gtcaccgtgt cctgcactgt tttcgcatca gcgccggggt aggaggcgga
     3121 gatcgttact gccggcggtg caatcgtagg atattgcgcc accggcagtt tgaggatcgc
     3181 cagcccccct gccaacatga tgataatggc gatcacccac gcaaaaatcg ggcgatcgat
     3241 aaagaaatta ggcatgtctt aacggctcct gtttaagtta agacttggac tgttcaggct
     3301 gagcaccgct tgcggcttgc tggttattat cagcggtaac ttcttgtgct tttacctgga
     3361 caccaggacg cactttctgc agcccactta ttactacgcg atcgcctgct ttcagacctt
     3421 ctgtcaccag ccacttatcg ccaatagcct ggcttgcaac gatcggacgg gtttccactt
     3481 tgtcatccgc gccaaccacc agtacggtgg catcgccacg cggcgtacgg gttacgccct
     3541 gttgcgggac taaaatagcg tttggattaa gcccttcttc cagacgtgcg cgcacgaaca
     3601 tacccggcag cagagtgtga tccgggttcg ggaagatagc gcgtagggtg atagacccag
     3661 tggtctgatc aacggtaacg tcagagaatt ccagcgtacc gtcctgcggg aacttaatgc
     3721 cgtcactggt gatcagcgac actttggctt tgccgttctc ttgtttcagc gtgccattcg
     3781 ccagttcctg tttcaggcgc aggaagtcgt tgctggactg ggtcacatca acgtagatcg
     3841 gatcaagttg ctgcacggtt gccagcgcag tcgcctgacc gttctgtacc aatgcgcctt
     3901 ccgtcacgtt cgacttacca atgcgaccgc taatcggaga ggtgactttg gtgtaagcca
     3961 gattgatccg cgcagtttca acggcagctt tcgccgcagt taccgcagca ttcgcctgtt
     4021 gcgcatcagc cagagcctga tcgtactctt gcttactgat gtactgagta ccgagcagtt
     4081 tctgataacg attcaccgtc aattgcgcga tattggctgc agcctgggct ttcgccagat
     4141 cacctttcgc actgtcgtat gtcgcctgat aggtcgcagg atcaatctga tagagagaga
     4201 cacctgcttc gatgtcgcta ccttctttga aattacgctt caggataatc ccgctaactt
     4261 gaggacgaac ttctgcgatc cggtaggcac tggtgcgacc cggaagctcg gttgtgatct
     4321 gcagaggttc agttttgact gttactacgc caacggcggg catctgctgg ccaccttgtt
     4381 gggcctgttt gtcgtcacat cctgttaggg ctaagctgcc tgagagcatc agaacgaccg
     4441 ccagaggcgt aaaccctctg tttttgttca tatgtaaacc tcgagtgtcc gatttcaaat
     4501 tggtcaatgg tcaaaagtta ataaacccat tgctgcgttt atattatcgt cgtgctatgg
     4561 tacatacatt cacaaatgta tgtaaatcta acgcctgtaa attcacgaac atatggcacg
     4621 aaaaaccaaa caagaagcgc aagaaacgcg ccaacacatc ctcgatgtgg ctctacgtct
     4681 tttctcacag cagggggtat catccacctc gctgggtgag attgcaaaag cagctggcgt
     4741 tacgcgcggt gcaatctact ggcattttaa agacaagtcg gatttgttca gtgagatctg
     4801 ggaactatca gaatccaata ttggtgaact agagcttgag tatcaggcaa aattccctgg
     4861 cgatccactc tcagtattaa gagagatatt aattcatgtt cttgaatcca cggtgacaga
     4921 agaacggcgt cgattattga tggagattat attccacaaa tgcgaatttg tcggagaaat
     4981 ggctgttgtg caacaggcac aacgtaatct ctgtttggaa agttatgacc gtatagaaca
     5041 aacgttaaaa cattgtattg aagcgaaaat gttgcctgcg gatttaatga cgcgtcgggc
     5101 agcaattatt atgcgcggct atatttccgg cctgatggaa aactggctct ttgccccgca
     5161 atcttttgat cttaaaaaag aagcccgcga ttacgtcgcc atcttactgg agatgtatct
     5221 cctgtgcccc acgcttcgta atcctgccac taacgaataa ccctgaatct gactccagga
     5281 tttttcctgg acattttcgt cgttgctatt ctggttcact gcgtcgtgat attcttgcgg
     5341 tttgactttt tcaggtcgtt cttcaggttc agaaaccttc attcatcatg actatgttcc
     5401 agtattacaa acgatcacgg cattttgttt tttcagcatt tattgctttt gtttttgtct
     5461 tgttatgcca gaacacggcg tttgcgcggg cgtcatcgaa tggtgatctg ccgacaaaag
     5521 cggacctgca ggcgcaactt gactcactaa ataaacaaaa agatctttct gctcaggaca
     5581 aactggtgca gcaggatctg acagatacat tagccaccct cgataaaatc gatcgcgtaa
     5641 aagaagagac cgttcagcta cggcaaaaag tcgctgaagc gccggaaaaa atgcgccagg
     5701 cgaccgcggc gttaacagca cttagcgatg tcgataacga cgaagaaacg cgcaaaattc
     5761 tgagcacgct ttcgttgcgc cagctggaaa ctcgcgttgc ccaggcgctg gacgatttgc
     5821 aaaacgcaca aaacgatctg gcgtcttata acagccagct ggtttcgtta cagacgcagc
     5881 ccgaacgcgt gcaaaatgcg atgtataacg cttcgcagca gctgcaacaa attcgcagtc
     5941 gtctggatgg gactgatgtc ggcgagacgg ccttacgtcc cagccagaaa gtgttaatgc
     6001 aggctcagca ggcgttgctg aatgcggaga ttgaccagca gcgtaaaagc ctggaaggga
     6061 ataccgtctt gcaggatacc ttgcaaaagc aacgtgatta cgtgacggcg aacagcgccc
     6121 gtctggagca ccagttgcag ctgttgcaag aagcggtaaa cagcaagcgc ctgactttaa
     6181 ccgaaaaaac ggcgcaggaa gccgtctccc cggatgaagc cgcgcgtatt caggctaatc
     6241 cgctggtgaa gcaggaactg gaaattaacc agcagttaag tcagcgtctg attaccgcga
     6301 ctgaaaacgg taatcagttg atgcagcaaa acattaaagt caaaaactgg ctggagcggg
     6361 cgctgcaatc ggaacgcaat attaaagagc agattgccgt cctgaagggc agcctgctgt
     6421 tgtctcgtat cctttaccag caacaacaaa cgctgccctc ggcggatgaa ctggaaaaca
     6481 tgaccaaccg catcgcggat ttgcgtctcg aacagtttga agttaaccag cagcgtgatg
     6541 cactcttcca gagcgatgcg ttcgtcaaca aactggaaga aggtcacacc aacgaagtca
     6601 acagcgaagt tcacgatgcg ttattgcaag tggttgatat gcgtcgcgaa ttgctggatc
     6661 aactcaacaa acagttgggt aaccagctga tgatggccat taacctgcaa atcaaccagc
     6721 agcagttaat gagtgtgtcg aaaaacctga aatccatcct gactcagcaa atcttttggg
     6781 tgaacagtaa ccgtccaatg gactgggact ggatcaaagc gttcccgcaa agcctgaaag
     6841 atgaatttaa gtcgatgaaa attacggtta actgggaaaa agcctggccc gccgttttta
     6901 tcgctttcct cgctggtttg ccgctgctgt tgattgccgg gctgatccac tggcgtctgg
     6961 ggtggctgaa agcgtatcaa caaaaactgg cttccgctgt gggttccctg cgtaacgaca
     7021 gccagctcaa cacaccaaaa gcgatcctta tcgacctgat ccgtgcactg ccggtgtgcc
     7081 tgattattct cgcggttggc ctgattctgt tgaccatgca gctcaacatc agcgaactgc
     7141 tatggtcgtt cagcaaaaaa ctggcgattt tctggctggt gtttggcctg tgctggaaag
     7201 tgctggagaa gaacggcgtt gccgtgcgtc acttcggcat gccggaacag cagaccagcc
     7261 actggcgtcg gcaaattgtc cgcatcagtc tcgcgttgct gcctatccat ttctggtctg
     7321 tggtggcaga actttccccg ctgcatctga tggatgatgt gctggggcag gcgatgattt
     7381 tcttcaacct gctgctgatt gccttcctgg tatggccaat gtgccgcgaa agctggcgtg
     7441 ataaagagtc gcacaccatg cgactggtca ccattaccgt gctgtcgatt atcccgattg
     7501 cattgatggt gctgactgcc acgggttatt tctacaccac gctgcgcctc tccgggcgtt
     7561 ggattgaaac cgtttatctg gtgatcatct ggaacttgct ctaccagacg gtattgcgcg
     7621 gcttaagcgt agcggcgcgg cgtatcgcct ggcgtcgtgc gctggcgcgt cggcagaatc
     7681 tggtgaaaga gggcgcagaa ggtgccgaac cgccggaaga acccaccatt gcactggaac
     7741 aggttaacca gcagacgctg cgtattacca tgttgctgat gtttgcgctg ttcggtgtca
     7801 tgttctgggc aatttggtcc gatttgatca ccgtgttcag ctatctcgac agcattacgc
     7861 tgtggcatta caacggtact gaagctggtg ctgcggtggt gaaaaacgtc accatgggta
     7921 gtctgttgtt tgcgattatc gcctcaatgg tggcctgggc gttgattcgc aacctgcctg
     7981 gtttgctgga agtgctggtg ctctcgcgac tgaatatgcg ccagggcgcg tcgtatgcca
     8041 ttactaccat ccttaactac atcattattg ctgttggtgc gatgacggtg ttcggatcgc
     8101 tgggcgtctc ttgggataaa ctccagtggc tggccgcagc attatccgta ggtcttggtt
     8161 ttggtttaca agaaattttc ggtaacttcg tctccggttt gatcattctg ttcgaacgtc
     8221 cggtgcgtat tggcgatacg gtaaccattg gtagcttctc ggggacggta agtaaaatcc
     8281 gtattcgtgc gacaacgatt accgatttcg atcgcaaaga agtgatcatc ccgaacaaag
     8341 cgtttgttac cgagcgtctg atcaactggt cgttgactga cactactacg cgtctggtga
     8401 tccgtctcgg cgtggcctat ggctccgatc tggaaaaagt gcgtaaagtg ttactgaagg
     8461 cggcgactga gcacccaagg gtgatgcacg aaccaatgcc ggaagtcttc tttacggcat
     8521 ttggtgccag cacgttggat catgagctgc gtctgtatgt gcgtgaactg cgtgaccgta
     8581 gtcgtactgt cgatgagctg aaccgtacta tcgatcagct gtgccgtgaa aacgacatca
     8641 acattgcctt taaccagctt gaagtgcatc tgcacaacga gaagggcgat gaggtgacgg
     8701 aagtaaaacg cgactacaaa ggcgacgatc cgacgccagc ggtagggtaa ggacaataaa
     8761 gggcgacagt gatgccgccc tttatttttt actcgctttg tgaagcctca tcgctctgta
     8821 acttcttttc ataatcacgc tttacaatat ccagcgcgcg cagcacggtg cgggctggga
     8881 tctgattttc ttccagcagc acaatcaaat cgacagccag tttgacatcg tcaggggcat
     8941 tttccagtga catactctct ccattgctag cgggttaaac gcgctaacct gttttcgatt
     9001 ttttccagcg catggcggca gcgtgccagg cgcgcttcat aggcttccac ttcacgatgc
     9061 agcgtttgct gttccacgag atcggtcacc cgcgccaggc gggctctacg ttcggcaacc
     9121 atctcacgta gccgccgctc aaaatcctga tgctgaatac gtttacgctg ccagcgggca
     9181 atttggggtg gtgcgctatc ccactcgcgt agcgaccagg ctgtggcttc acgcgctata
     9241 gcctccagtt gcgccgccag atgttctgcc agccaggcga cttgcggcag ttgctgttgc
     9301 tcaactgcat gacgaagcgc agccagatta tcgcccgcct tgtcgagaca agcctgtagt
     9361 gttgtcgcac gagtctgaaa aagatgcctg ttgaaacgag cacttagcgt ggcgaactgt
     9421 gacaccgggg cacaacgctg acgcagcgta gcgagctgtc cttccagttt ttccagcagc
     9481 agggcggttt tcacgataag acctcaatga aaatgataat tgttatgcta aagtagccac
     9541 tctgtaagct gacaacactc aatatgcaac gaatcatttt aatcatcatt ggctggctgg
     9601 cggtagtgct gggcacgctg ggcgtggtat taccggtatt accgacgacg ccgtttatcc
     9661 tgctggcggc ctggtgcttt gcccgttctt ccccgtgctt tcacgcctgg ttgctgtacc
     9721 gctcatggtt tggcagctat ctacgtttct ggcagaaaca tcatgcgatg ccgcgcggcg
     9781 tcaaaccgcg ggcgattttg cttattttgc tcacgtttgc catttctctg tggttcgtcc
     9841 agatgccatg ggtgcgcatc atgttgctgg taattctcgc ctgtttgctt ttctatatgt
     9901 ggcgaattcc ggtgattgat gaaaagcaag aaaagcactg aagcacaaca atcgcagttg
     9961 caattattgc gtacagccag tacattctgg cgttttcgag cacaggcgca ggcggtcaaa
    10021 ggttaaacaa ctgttacttt tgatacgttt aaaacgcgcc gtgagtacca ccgtaacaag
    10081 caggcataca cttatgaccg cgactgcaca gcagcttgag tatctcaaaa atagcatcaa
    10141 aagcattcag gactacccaa aacccggcat tcttttccgc gatgtcacca gcttactgga
    10201 agacccgaaa gcttacgctc tcagcatcga cttgctggtt gagcgttaca aaaatgcggg
    10261 cattaacaaa gttgtcggca ccgaagcgcg tggcttcttg tttggcgctc cggtagctct
    10321 gggtctgggc gttggctttg taccggtccg taaaccgggc aaactgccgc gtgaaaccat
    10381 cagtgaaact tacgatctgg aatacggcac cgatcagctg gagatccacg ttgatgccat
    10441 caaaccgggc gacaaagttc tggtggtgga cgatctgctg gcaaccggcg gcactatcga
    10501 agcgaccgtt aaactgatcc gtcgtctggg tggtgaagtg gctgacgctg cgttcattat
    10561 caacctgttc gatctcggcg gcgaacagcg tctcgaaaaa cagggcatta ccagctacag
    10621 ccttgtcccg ttcccgggcc attaattatc gccagtcttg tgctgcccac gctacggaca
    10681 gcacaagatg tgcattcagc ctcgccgttc tgacggggct gtgttagcat taccccttcg
    10741 agaatccacc ttccagcgtt tcagagcctg ccaatgagtt atcaggtctt agcccgaaaa
    10801 tggcgcccac aaacctttgc tgacgtcgtc ggccaggaac atgtgctgac cgcactggcg
    10861 aacggcttgt cgttagggcg tattcatcat gcttatcttt tttccggcac ccgtggcgtc
    10921 ggaaaaacct ctatcgcccg actgctggcg aaggggctaa actgcgaaac cggcattacc
    10981 gcgacgccgt gcggcgtgtg cgataactgt cgtgaaatcg agcaggggcg ctttgtcgat
    11041 ctgattgaaa tcgacgccgc ctcgcgcacc aaagttgaag atacccgcga cctgctggat
    11101 aacgtccagt acgctccggc gcgtggtcgt ttcaaagttt atctcatcga cgaagtgcat
    11161 atgctgtcgc gccacagctt taacgcactg ttaaaaaccc ttgaagagcc gccggagcac
    11221 gttaagtttc tgctggcgac gaccgatcca cagaaattgc cggtgacgat tttgtcgcgc
    11281 tgtttgcaat ttcatctcaa ggcgctggat gtcgagcaaa ttcgtcatca acttgagcac
    11341 atcctcaatg aagaacatat cgctcacgag ccgcgggcgc tgcaattgct ggcgcgcgcc
    11401 gctgaaggca gcctgcgaga tgccttaagt ctgaccgacc aggcgattgc cagcggtgac
    11461 ggccaggttt caacccaggc ggtcagtgcg atgctgggta cgcttgacga cgatcaggcg
    11521 ctgtcgctgg ttgaagcgat ggtcgaggcc aacggcgagc gcgtaatggc gctgattaat
    11581 gaagccgctg cccgtggtat cgagtgggaa gcgttgctgg tggaaatgct cggcctgttg
    11641 catcgtattg cgatggtaca actttcgcct gctgcacttg gcaacgacat ggccgccatc
    11701 gagctgcgga tgcgtgaact ggcgcgcacc ataccgccga cggatattca gctttactat
    11761 cagacgctgt tgattggtcg caaagaatta ccgtatgcgc cggaccgccg catgggcgtt
    11821 gagatgacgc tgctgcgcgc gctggcgttc catccgcgta tgccgctgcc tgagccagaa
    11881 gtgccacgcc agtcctttgc acccgtcgcg ccaacggcag taatgacgcc aacccaggtg
    11941 ccgccgcaac cgcaatcagc gccgcagcag gcaccgactg taccgctccc ggaaaccacc
    12001 agccaggtgc tggcggcgcg ccagcagttg cagcgcgtgc agggagcaac caaagcaaaa
    12061 aagagtgaac cggcagccgc tacccgcgcg cggccggtga ataacgctgc gctggaaaga
    12121 ctggcttcgg tcaccgatcg cgttcaggcg cgtccggtgc catcggcgct ggaaaaagcg
    12181 ccagctaaaa aagaagcgta tcgctggaag gcgaccactc cagtgatgca gcaaaaagaa
    12241 gtggtcgcca caccgaaggc gctgaaaaaa gcgctggaac atgaaaaaac gccggaactg
    12301 gcggcgaagc tggcggcaga agccattgag cgcgacgcgt gggcggctca ggtgagtcaa
    12361 ctttcgctac caaaactggt cgaacaggtg gcgttaaatg cctggaaaga ggagagcgac
    12421 aacgcagtat gtctgcattt gcgctcctct cagcggcatt tgaacaaccg cggtgcacag
    12481 caaaaactgg ctgaagcgtt gagcatgtta aaaggttcaa cggttgaact gactatagtt
    12541 gaagatgata atcccgcggt gcgtacgccg ctggagtggc gtcaggcgat atacgaagaa
    12601 aaacttgcgc aggcgcgcga gtccattatt gcggataata atattcagac cctgcgtcgg
    12661 ttcttcgatg cggagctgga tgaagacagt atccgcccca tttgatcgta agcacagctt
    12721 acgttcgtca tccttaacgt gattgagaga gaaacctatg tttggtaaag gcggtctggg
    12781 taacctgatg aagcaagccc agcagatgca agaaaaaatg cagaaaatgc aggaagagat
    12841 cgcgcagctg gaagttaccg gcgaatctgg cgcaggtctg gtaaaagtga ccatcaacgg
    12901 tgcacataac tgccgtcgcg tagagatcga cccgagcctg ctggaagacg acaaagagat
    12961 gctggaagac ctggtggctg cagcattcaa cgacgcagca cgtcgtattg aagaaacgca
    13021 gaaagaaaaa atggcttctg tatcctccgg aatgcagctg ccgcctggct ttaagatgcc
    13081 gttctgatgc aaaccagccc gctgttaaca cagcttatgg aagcactgcg ctgtctgccg
    13141 ggcgttggcc cgaagtcggc gcagcgtatg gcgttcacac tgcttcagcg cgatcgtagc
    13201 ggcgggatgc gtctggcgca ggcgctcacc cgggcgatgt cggaaatcgg ccactgcgcc
    13261 gattgccgca ccttcaccga acaggaagtc tgtaacatct gttcgaatcc gcgtcgtcag
    13321 gaaaacggtc aaatctgcgt ggtggagagt ccggcggaca tttacgccat tgagcagacg
    13381 gggcagtttt caggtcgtta ttttgtgttg atgggacatc tgtcaccgct ggacggcatc
    13441 ggtccggatg atatcggcct tgatcgtctg gaacagcgtc tggcagagga aaaaatcact
    13501 gaagtgatcc tcgccaccaa ccctacggtt gaaggtgaag ctaccgctaa ctacattgcc
    13561 gagctttgcg cgcaatatga cgtggaagcc agccgaatcg ctcatggcgt accggttggc
    13621 ggcgaactgg aaatggtcga tggcaccacg ctgtcacact cccttgccgg gcgtcataag
    13681 attcgttttt aagcaaacga gagcaggatc acctgctctc gcttgaaatt attctccctt
    13741 gtccccatct ctccctcatc ctgtttttaa ccttaaaatg gcattattga ggtagaccta
    13801 catgaaagga caagaaactc gtggttttca gtcagaagtg aaacagcttc tgcacctgat
    13861 gatccattct ctctattcca ataaagaaat cttcctgcgt gagcttatct ctaacgcctc
    13921 cgatgcggcg gacaagttgc gtttccgtgc gctctctaac ccggacctgt acgaaggtga
    13981 tggcgaactg cgcgttcgtg tctctttcga taaagacaag cgcacgctga ccatctccga
    14041 taacggcgtg gggatgaccc gcgacgaagt gattgaccat ctggggacta tcgctaaatc
    14101 cggtaccaaa tcattcctcg aatccctggg ttctgaccag gcgaaagaca gccagctgat
    14161 cggtcagttt ggtgttggtt tctactctgc gtttatcgtg gccgacaaag tgaccgtgcg
    14221 tactcgcgcg gcaggcgaaa aaccagaaaa cggcgtcttc tgggaatcgg ctggcgaagg
    14281 tgaatatacc gttgccgaca tcaccaaaga agatcgtggt actgaaatca ccctgcatct
    14341 gcgtgaaggc gaagacgagt tcctcgatga ctggcgcgtg cgttccatca tcagcaaata
    14401 ctccgaccat atcgcgctgc cggtagagat cgaaaaacgc gaagagaaag acggcgaaac
    14461 cgttatctcc tgggagaaaa ttaacaaagc gcaggcgctg tggactcgta acaagtcgga
    14521 aatcaccgat gaagagtaca aagagttcta caaacacatc gcccacgatt ttaacgatcc
    14581 gctgacctgg agccacaacc gtgttgaagg taagcaggag tacaccagcc tgctgtacat
    14641 tccgtcccag gctccgtggg atatgtggaa ccgcgatcat aaacacggcc tgaaactgta
    14701 tgttcagcgt gtgttcatca tggacgacgc agaacagttc atgccgaact atctgcgctt
    14761 cgtgcgtggt ctgattgact ccagcgatct gccgctgaac gtttcccgtg aaatcctcca
    14821 ggacagcacg gtaacgcgta acctgcgcaa tgcgctgacc aagcgtgtgc tgcaaatgct
    14881 ggaaaaactg gcgaaagacg acgcggaaaa ataccagacc ttctggcaac agtttggcct
    14941 ggtactgaaa gaaggtccgg cggaagattt cgctaaccag gaagcgatcg ccaaattgct
    15001 gcgttttgct tctacccata ccgattcttc tgcgcagacc gtgtctctgg aagactacgt
    15061 ttcccgcatg aaagaagggc aggagaaaat ctactacatc actgcagaca gctatgcggc
    15121 agcgaagagc agcccgcacc tggaactgct gcgtaagaaa ggcatcgaag ttctgctgct
    15181 ttccgaccgc atcgatgagt ggatgatgaa ctatctgact gagttcgacg gtaaaccgtt
    15241 ccagtcggtg tctaaagttg acgagtcgct ggaaaaactg gctgacgaag ttgatgagag
    15301 cgcgaaagaa gcggagaaag cactgactcc gttcatcgac cgtgtgaaag ccctgctcgg
    15361 cgagcgcgtg aaagatgtcc gtctgactca ccgtctgacc gatacgccag cgatcgtttc
    15421 gaccgacgcg gacgaaatga gcactcagat ggcgaaactg ttcgctgcgg cgggccagaa
    15481 agtgccagaa gtgaaataca tcttcgaact gaacccggat cacgtactgg tgaaacgtgc
    15541 ggcagatact gaagatgaag cgaagttcag cgagtgggta gaactgctgc tggatcaggc
    15601 actgctggca gaacgcggca cgctggaaga tccgaacctg tttattcgtc gtatgaacca
    15661 gctgctggtt tcctgatgta atgccggatg accttcgtgt catccggcat ttttcttttc
    15721 attatctgca ctttccgcaa attatctcgc cattaaccgt ttcagcccct ggtgcctttc
    15781 ttgaggcaat cgcctgatgg tggtatcgtt tatcgctttt tcaaaaaatt cgacacattt
    15841 taaggggatt ttcgcaatgc gtatcattct gcttggcgct ccgggcgcgg ggaaagggac
    15901 tcaggctcag ttcatcatgg agaaatatgg tattccgcaa atctccactg gcgatatgct
    15961 gcgtgctgcg gtcaaatctg gctccgagct gggtaaacaa gcaaaagaca ttatggatgc
    16021 tggcaaactg gtcaccgacg aactggtgat cgcgctggtt aaagagcgca ttgctcagga
    16081 agactgccgt aatggtttcc tgttggacgg cttcccgcgt accattccgc aggcagacgc
    16141 gatgaaagaa gcgggcatca atgttgatta cgttctggaa ttcgacgtac cggacgaact
    16201 gatcgttgac cgtatcgtcg gtcgccgcgt tcacgcgccg tctggtcgtg tttatcacgt
    16261 taaattcaat ccgccgaaag tagaaggtaa agacgacgtt accggtgaag aactgactac
    16321 ccgtaaagac gatcaggaag aaaccgtacg taaacgtctg gttgaatacc atcagatgac
    16381 agcaccgctg atcggctact actccaaaga agcggaagcg ggtaacacca aatacgcgaa
    16441 agttgacggc accaagccgg ttgctgaagt tcgcgctgat ctggaaaaaa tcctcggcta
    16501 atcaagccgc agcagatctg ctttcgggca ggtctgctca tccacctcag gcaatacctc
    16561 tcacagcaat tagttcttct tcctcacttt tccgctacaa ttatcaacaa gttgaatcga
    16621 taagaggcgg taatgcgtca gactaaaacc ggtatcctgc tggcaaacct gggtacgccc
    16681 gatgccccca cacctgaagc ggtaaaacgc tatctgaaac aatttttaag cgacagacgc
    16741 gtggttgata cctcacggtt gttatggtgg ccattgctgc gcggcgtgat tttgccgctg
    16801 cgctcgccgc gtgtggcgaa gctgtatgcc tctgtctgga tggaaggtgg ctcgccgctg
    16861 atggtttaca gccgccagca acagcaggcg ctggcacaac gtttaccgga gacgcccgta
    16921 gcgctgggaa tgagctacgg ctcgccatca ctggaaagcg ccgtagatga actcctggca
    16981 gagcatgtag atcatattgt ggtgctgccg ctttatccgc aattctcctg ttctacggtc
    17041 ggtgcggtat gggatgaact ggcacgcatt ctggcgcgca aacgtagcat tccggggata
    17101 tcgtttatac gtgattacgc cgataaccac gattacatta atgcactggc gaacagcgta
    17161 cgcgcttctt ttgccaaaca tggcgaaccg gatctgctgc tgctctctta tcatggcatt
    17221 ccccagcgtt atgcagatga aggcgatgat tacccgcaac gttgccgcac aacgactcgt
    17281 gaactggctt ccgcattagg gatggcaccg gaaaaagtga tgatgacctt tcagtcgcgc
    17341 tttggtcggg aaccctggct gatgccttat accgacgaaa cgctgaaaat gctcggagaa
    17401 aaaggcgtag gtcatattca ggtgatgtgc ccgggctttg ctgcggattg tctggagacg
    17461 ctggaagaga ttgccgagca aaaccgtgag gtcttcctcg gtgccggcgg gaaaaaatat
    17521 gaatatattc cggcgcttaa tgccacaccg gaacatatcg aaatgatggc taatcttgtt
    17581 gccgcgtatc gctaaagctg agcggtaaag aactgagcgc cgtcgcgaag agcctcgtcg
    17641 gcggttttca tcatccgtga ataatgcaaa aaggcgtgca gcgtgcctgg gtagagtttg
    17701 aactcacagg gctgctgatg cgccgctaac gtctggtaaa gcagacggct gtcatccagc
    17761 agcggatcga actccgcccc ggcaataaaa cagggcggaa cttcgcgagt gagatcatta
    17821 ttaaacagac agtaatacgg cgactcgcgg tccgcgtcgt tgcttaaata cgcctcttca
    17881 tacatctgca aatcctgttg cgttaagcca tcccagacac cgcccaacag acgacgagtc
    17941 acggaatccc gtaatccgta aagcccatac cacagcaaaa cgcccgcaac tttaccgcaa
    18001 tcgatctgtt tatcacgcaa ccacaacgca ctggcgagcg ccagcatggc acctgcggaa
    18061 tcaccggcaa agccaatgcg ggacatattg atttgataat cctccgcctg ctggtggaaa
    18121 taacaacaag cagccacaat ttcctctatc acttgcggaa aacgcgcttc aggtgaaagg
    18181 gtgtaatcaa taccaatcac cgtacattgg ctgtagcttg ccagcaggcg catgatgcga
    18241 tcgtgggtat cgagattgcc gagaataaaa ccgcctccat gcaaataaaa tagcgttgcc
    18301 gggctatctg gctgcggaca aaagagacgt gtttccacct gcccatattt tgttggaacc
    18361 atgtaagctt tggttgccat ttctggagcg cccgcattcc agaatcggcg ctcaagcgtg
    18421 taatactgtc gttgctcagc aatcgttccc gttgcgggcc agggcggtaa atccggctga
    18481 agagtattca caacggtctt catttcagca gaaataaggt ccagaacagg taatttgttt
    18541 tccggcttca tacaaaaact cctttcaaat tacgtcattg taaggaacca ctgccatgaa
    18601 aatgcgatcc cgcctgctga tattgaaact ggctgcgtct cgcgcgctcc cgtcagattg
    18661 tgttaacatt cgccgctcag ttaaccaccc gtaaaaacaa ccatgaaatt tcccggtaaa
    18721 cgtaaatcca aacattactt ccccgtaaat gcacgcgatc cgctgcttca gcaattccag
    18781 ccagaaaacg aaaccagcgc cgcctgggta gtgggtatcg atcaaacgct ggtcgatatt
    18841 gaagcgaaag tggatgatga attcattgag cgttatggat taagcgccgg gcattcactg
    18901 gtgattgagg atgatgtagc cgaagcgctt tatcaggaac taaaacagaa aaacctgatt
    18961 acccatcagt ttgcgggtgg caccattggt aacaccatgc ataactactc ggtgctcgcg
    19021 gacgaccgtt cggtgctgct gggcgtcatg tgcagcaata ttgaaattgg cagctatgcc
    19081 tatcgttacc tgtgtaacac ctccagccgt accgatctta actatctaca aggcgtggat
    19141 ggcccgattg gtcgttgctt tacgctgatt ggcgagtccg gggaacgtac ctttgctatc
    19201 agccctggcc acatgaacca gctgcgggct gaaagtattc cggaagatgt gattgccgga
    19261 gcctcggctc tggttctcac ctcttatctg gtgcgttgca agccgggtga acccatgccg
    19321 gaagcaacca tgaaagccat tgagtacgcg aagaaatata acgtaccggt ggtgctgacg
    19381 ctgggaacta agtttgtcat tgccgagaat ccacagtggt ggcagcaatt cctcaaagac
    19441 cacgtctcta tccttgcgat gaacgaagat gaagccgaag cgttgaccgg agaaagcgat
    19501 ccgttgttgg catctgacaa ggcgctggac tgggtagatc tggtgctgtg caccgccggg
    19561 ccaatcggct tgtatatggc gggctttacc gaagacgaag cgaaacgtaa aacccagcat
    19621 ccgttgctgc cgggcgctat agccgaattc aaccagtatg agtttagccg cgccatgcgc
    19681 cacaaggatt gtcagaatcc gctgcgtgtc tattcgcaca ttgcgccgta catgggcggg
    19741 ccggaaaaaa tcatgaacac caacggagca ggggatggcg cactggcagc gttgctgcat
    19801 gacattaccg ccaacagcta ccatcgtagc aacgtaccaa actccagcaa acataaattc
    19861 acctggttaa cttattcatc gttagcgcag gtgtgtaaat atgctaaccg tgtgagctat
    19921 caggtactga accagcattc acctcgttta acgcgcggct tgccggagcg tgaagacagc
    19981 ctggaagagt cttactggga tcgttaagtt atcgtcggtt cgtaggccag ataaggcgtt
    20041 cacgccgcat ctggcatttt gctctcaatg cctgatgcga cgctggcgcg tcttatcatg
    20101 cctacatatt tttcatattt tacatccggc aaccaccgtt taccccgtca ccacttcacc
    20161 cgccggtggc gtttccagca gttccagcat agtacgggca atttcacgct cgcccatcac
    20221 tacttgattc gcaccacgtt cggtgatata cgccacttca tcgtcataat gggcgcgggc
    20281 aataatctca atatccggat ttttcgcgcg ggcagatgcc acaatctcac ccgcttcata
    20341 accgttgggg atcgtcagga tcagccattt tgcacattcc agatgcgcca gttgcataat
    20401 ttcttcgttc gccgcattgc ccaatactgc gcggaccccg cgctctcgca gctcatcaac
    20461 acgggttcgt gacgtctcaa tcaccaccag cggaatatca gaggcgagca atttctcccc
    20521 cagcaggctg cctacacgac cgtaacccac caatagcgca tggttgcaaa tatccactgg
    20581 gatctgcttc tcttcttcga ttgcctcttc cagcgtctgc tcttccagcg tttcggtctt
    20641 cgccagatat ttctccagta gtgcgaacag tactgggttg agcataatcg acaggatcgc
    20701 ccctgccagt accaggtttt gtccggcctg cggcagtaaa ttcaatgcca ttcccagtcc
    20761 cgccaggata aacgcgaact caccaatctg cgccaggctg gcggcgatag ttaatgccgt
    20821 acgttgggag tgaccaaaca gtcgcaccag gaaaaacgcg gctaacgact taccaaacag
    20881 aataatcgcc agcgtcgcca gcactgccag cggttgctga atcagaatta acggatcaaa
    20941 caacatcccg acggagacaa aaaacagcac cgcaaacgcg tcgcgcaatg gcagcgtatc
    21001 gtgggcggca cggtgactca gttcagactc gttcagtacc atcccggcaa agaacgcacc
    21061 gagtgcaaag gagacatcaa acagctctac cgcaccaaag gcaatcccta acgccagcgc
    21121 cagcaccgac agggtaaaca gctcgcgaga accggttgcc gcgctgcgtg ccataatcca
    21181 cggcaccaga cggcgaccta ccagcatcat aatggcgata aacgcgatca ctttgccgat
    21241 ggtgatcccc atatcgactg caagagtggc aaagcccaca tcgccctgtt ccatcattcc
    21301 tgccactgcg ggcagcaaca ccagcgtcag aaccattacc aggtcttcca caatcaacca
    21361 accgatggcg atttgcccac gctgactgtc aattaattgc cgttcttcaa gtgcgcgcag
    21421 taacaccacg gtactggcgg tggaaagaca taaaccgaac acgataccgg tcattaacga
    21481 ccagcccagc acggcagaga gcgccatacc cagcagcgtc gccacggcta tctgggcgat
    21541 cgcaccggga atggcgatgg cctttaccgc catcaaatcc ttgagcgaaa agtgcagacc
    21601 cacgccaaac atcaacagaa tgacgcccag ttcagccagt tccggcgcaa gcttggtatc
    21661 ggcaacaaag cccggagtga atggtcctgc cagcacaccc gctaacagat atcccaccag
    21721 aggagaaata cgtagtttat tggccagcat gccgaggata aaggcgagca caaggccgcc
    21781 aacaatggtg gtgataagcg gggtggcgtg atgcattccg tctccttttc ctggtggtta
    21841 ttgtccattt ttggccggga aaaccaaaat tacaggtaat agtttatgac aatttcattg
    21901 atgatgttca tgaataatta ttgaattttg cagaaaaatg gaattagctg caaaaaagca
    21961 cggatcggaa aacagaaggc ggtttaacag aggaaaggct tatggcgact gtaaaacgat
    22021 gcagccaaag tttgcctttg gctgcaatga aatcagtctt tatgccggtt atcaggcagg
    22081 aatatggtca acatccccaa tagtggcagg aaagcacata ttttatagac taactcgatg
    22141 ctggtgtgat cggcgataag ccccagaact gccgctccca gacctcccat gccgaaagca
    22201 aaaccgaaaa agagtccaga aaccataccg atacgtcctg gaagcagctc ctgagcgtag
    22261 accagaatgg cagagaatgc cgaagcgagg ataaatccaa taatcaccgt taaaaccccc
    22321 gtccagtgca ggctggcgta gggtaaaatc agcgtaaacg gcgcaacgcc gaggatagag
    22381 ccccaaatca catatttccg cccaatttta tcccctacag gcccgccgat caccgtacct
    22441 gccgcaacgg caaacaggaa ggcaaacaga tgaagctgag cattctggat agataatccg
    22501 aatttttgca tcagataaaa ggtgtaatag ctgctgatgc tcgccatata gaaatatttc
    22561 gagaaaatga ggattaacag aatgctgacc gccagtacca ctttgttgcg cggcagtgga
    22621 ttgataatcg tcgctttggg ttttccttta ttcattcggt gctgtgccga gtaccaacgg
    22681 ctgatttgcg ccaacaccac gatcgccagc agtgccgcaa gcacaaacca ggcaacgttg
    22741 cctttgccat aaggcgcgat aatcaccgcc gccagcaagg gtcccaggga actgccaaag
    22801 ttgccgccga cctgaaagat agattgtgcc aggccatgcc gcccgccgga agccatacgg
    22861 gccacgcgag aagattccgg atgaaagacc gatgaaccgg taccgaccag cgccgccgcc
    22921 agcagaactg cgccaaaact gcccgccagc gcaagcagca ccagaccact taaggtaaag
    22981 cacatgccaa ttggcaacga ccacggcatc ggatatttat cggtccagta gccgaccact
    23041 ggttgcagta gcgaagaggc gagctggaag gtgagggtta tcatgccaat ctgcataaat
    23101 gtcagagaaa attctgactg aagcagagga taaatcgcca gaatcagcga ttggatcatg
    23161 tcgttcagca gatgtgagag gctgatagca cctaaaatac caaacgatgt tcgggccttg
    23221 gtcgttgacg cagccgcgcc cgccacaggc tgggtttgtt cactcattgc cataggaaag
    23281 tcactttttc agggttgcga tgttaagaat gatcttattt gtgattatta cccgactaac
    23341 atacctgtat gcgccgcctg aaggaagtct caacgctgaa tacagaattt ctaatctgga
    23401 tgcagattta tcttcaccgg acgcagactt gtctatgatg tcgcgtcata ctatttttca
    23461 acacgttgaa atcaggtcag ggagagaagt atgaaattat tgcagcgggg cgtggcgtta
    23521 gcactgttaa ccacatttac actggcgagt gaagctgctc tggcgtatga gcaggataaa
    23581 acctacaaaa ttacagttct gcataccaat gatcatcatg ggcatttttg gcgcaatgaa
    23641 tatggcgaat atggtctggc ggcgcaaaaa acgctggtgg atggtatccg caaagaggtt
    23701 gcggctgaag gcggtagcgt gctgctactt tccggtggcg acattaacac tggcgtgccc
    23761 gagtctgact tacaggatgc cgaacctgat tttcgcggta tgaatctggt gggctatgac
    23821 gcgatggcga tcggtaatca tgaatttgat aacccgctca ccgtattacg ccagcaggaa
    23881 aagtgggcaa agttcccgtt actttctgcc aatatctacc agaaaagtac cggcgagcgc
    23941 ctgtttaaac catgggcgct gtttaagcgt caggatctga aaattgccgt tattggtctg
    24001 acgacggatg acacagcgaa gttaggcaat ccagaaaact tcaccgatat tgagttccgt
    24061 aagcccgccg atgaagcgaa gctggtgatt caggagctgc aacagacaga aaagccagac
    24121 attattatcg cggcgacaca tatggggcat tacgataacg gcgatcacgg ttctaacgca
    24181 ccgggcgatg tggagatggc acgcgcgttg cctgccggat cgctggcgat gattgtcggc
    24241 ggtcactcgc aagatccagt ctgtatggca gcggaaaata aaaagcaggt agattacgtt
    24301 ccaggtacgc catgcaaacc ggatcaacaa aacggcatct ggattgtaca ggcgcatgag
    24361 tggggcaagt acgttggtcg ggccgacttt gagtttcgca acggcgaaat gaaaatggtt
    24421 aactaccagc tgattccggt gaacctgaag aagaaagtga cctgggaaga cgggaaaagc
    24481 gagcgcgtgc tgtacacccc tgaaatcgct gaaaaccagc aaatgatctc gctgttatcg
    24541 ccgttccaga acaaaggcaa agcgcagctg gaagtgaaaa taggcgaaac caatggtcgt
    24601 ctggaaggcg atcgtgacaa agtgcgtttt gtacagacca atatggggcg gttgattctg
    24661 gcagcacaaa tggatcgcac tggtgccgac tttgcggtga tgagcggagg cggaattcgt
    24721 gattctatcg aagcaggcga tatcagctat aaaaacgtgc tgaaagtgca gccattcggc
    24781 aatgtggtgg tgtatgccga catgaccggt aaagaggtga ttgattacct gaccgccgtc
    24841 gcgcagatga agccagattc aggtgcctac ccgcaatttg ccaacgttag ctttgtggcg
    24901 aaagacggca aactgaacga ccttaaaatc aaaggcgaac cggtcgatcc agcgaaaact
    24961 taccgtatgg cgacattaaa cttcaatgcc accggcggtg atggatatcc gcgccttgat
    25021 aacaaaccgg gctatgtgaa taccggcttt attgatgccg aagtgctgaa agcgtatatc
    25081 cagaaaagct cgccgctgga tgtgagtgtt tatgaaccga aaggtgaggt gagctggcag
    25141 taatccgaaa gtgccggatg ttcgcatccg gcacaatgct taatcgcggc gggcgatatc
    25201 agcaaatttg gcatcgagga tcttcgccag atcgcctgcc gccagttcga tatccagtcc
    25261 gcgcttgccg ccggaaacat aaatagtggc aaattcttgt gcgggggcgt cgataatcgt
    25321 tggcagacgt tttttctgcc ccagtgggct aatcccccca accaggtatc ccgtcgaacg
    25381 ctgcgcgacc atcggatcgg ccatctcaac tttcttggca cccagcgctt ttgctacttt
    25441 tttaaggtcc agttgacctg cgaccggcgt aacggccacg gcaaggtgtt tcatatcacc
    25501 gttcactgcg accagcagcg ttttgtagac ctgatccgga ttcaaaccta attttttgac
    25561 gacttcatcg ccaaaattgg tttcagccgg atcgtgctcg taggtatgga tttgaaacga
    25621 aatcttgttt ttttcgagtg atttaactgc gggtgtcatg gtaattttcc tgccttaaac
    25681 aaaaaaacga agcaagcata cgcctgatgg cggtccaaaa aaaagagtca tcttgcctaa
    25741 gagtattggc aggatggtga gattgagcga caatcgagtt acaccgtcgc ttaaagtgac
    25801 ggcataataa taaaaaaatg aaattcctct ttgacgggcc aatagcgata ttggccattt
    25861 ttttagcgca acatttgcgg taaattccct tctccataca ggtgtagtgc accgaccgcg
    25921 accacatatc gccccggcgg catggcgcgt aatttatccc gccaggcgag atttcgctga
    25981 tgcatcagca catcgtacag cgactgactg aacgtattgg gcagcgttat ttcattattt
    26041 tgcggcggcg cattcagcca ccagctcatc atttgttgca gcaaccgtgc gttggtatgc
    26101 cagtgggtca gcgtatcgtc cagcagcgcc agtcctttgt cagggagctg gagcaacatg
    26161 gcaatctggt tttccgcccc ttccagttca atcacgggtt tatgttgttg cttcgccgcc
    26221 tgcaatagct gataatcgat accgtattct gcccgcagcc ccagtttttg tgcctgcgtc
    26281 gcctgaagaa ccatcgcgat ttgccacagc ggttgggtag aaaagagtga gggagaaatg
    26341 cccatctcct ggctaatgtg ctgcagtttt tggagttgct cctcgctaat gcgctcttcc
    26401 agcgcctcgc aggcaggcaa attagcaaaa ggcgtatcgc tggtggaaac atccgcctcg
    26461 acgatcagcg catcggcgtt tttgagcttt ttgagcaaac gggtgggtag gggagccata
    26521 tcgtggctac ccatatgaat actgccaatc agatgaaaat ggcgattgcc gggaagagag
    26581 atatcaatgg caggccaggt gtaatgatta ccgcgcagcg cggcccaaag cgtttttacc
    26641 cggtacaaca gatccatacg acctcccttt gtgaaatatc atgctagcgc gcggggaggg
    26701 atggcgcaaa tggagttggg gacttgttta atgtgtttgt atgattcagt atgttcttgt
    26761 atcgctattt acagggaagc aacagttaaa aactatgaaa caggcaacaa gaaaaccgac
    26821 gacacccgga gatattcttc tctatgaata tctggaaccg ctagatttga aaatcaatga
    26881 gttagcagag ttgctgcatg ttcatcgtaa tagcgtcagt gcactgatca ataacaatcg
    26941 taaactcacc actgagatgg cattccgtct ggcgaaagtt tttgatacca cagtcgattt
    27001 ttggctaaac ctccaggcgg cagttgatct ttgggaagtt gaaaacaaca tgcgcaccca
    27061 ggaagaattg ggacggattg aaacagtggc tgaatatttg gcacgccgtg aagagcgtgc
    27121 aaaaaaggtc gcgtaaggcc aaaacatcgc ttcatctcgt aggccggata aggcgttcac
    27181 gccgcatccg gcatccgagc accattgcct gatgcgacgc ttacgcgtct tatcaggcct
    27241 acaaacctgt gcctgaaccg taggccggat aaggcgttta cgccgcatcc ggcagtcatg
    27301 cgtcgatgcc aaatgcgcca ccctaaagca gcgcatccgc aatgacgtac ttattccttc
    27361 ggtttaaacc gcagcaaccg gttggcgtta ctcactacgg taatcgacga gagcgccatt
    27421 gccgctccgg caactaccgg gttaagcagt gttccagtaa acggccacaa aataccggcg
    27481 gcgaccggaa taccgatact gttgtagata aacgcgccga gcaggttctg cttcatgttg
    27541 cgcagcgttg cgcgggaaat agcgagcgca tccgcaacgc ccatcaggct atggcgcatc
    27601 agggtaatcg ccgcggtttc aatggcaaca tcactgccgc cgcccattgc aatgccgaca
    27661 tccgcctggg ccagcgctgg cgcatcgtta atgccgtcgc ccaccattgc cacctgacgt
    27721 ccttcacttt gcaggcgttt aatcgcttcg gctttaccgt ccggcagcac cccggcgatc
    27781 acctcatcaa tccctgcttc tttggcgatc gcattggcgg tggttgggtt atccccggtc
    27841 aacatcacca gacgatatcc cgctttatgc agacgttgca gcgccgccac gctatcacta
    27901 cgcaacggat cgcgtacagc cagcagggct accgctttcc cgtcaaccgc cagcagcaca
    27961 ggcgttgccc cttgcgatgc ctgagctgaa atatctgctt caatcgcttt agtatcaact
    28021 tgctggtcat ttaacagcgc ctgattgccc agcaataacg catgaccttc agcttcaccg
    28081 ctcacgccca gcccgcgcaa tgtgcggaaa ccgttgacct gcggcagctg catatcgctt
    28141 gctttatcga ggatcgcccg cgccagcgga tggctggaac cttgttccag tgccgccgcc
    28201 agacgcagcg cctgcgcttc gtcaaaatca gcaaatgttt tcactgcgac aacctgcggc
    28261 ttcccttcgg tcagcgtccc ggttttatcg aacactacgg tatcgagtgt actagcgcgt
    28321 tgcagcgcgt cggcgtcccg caccagcacg ccaaactcag ccgcccgccc gacgccggaa
    28381 ataatcgaca tcggcgtcgc cagccccagc gcacacggac aggcaataat cagcaccgtg
    28441 gtggcaatca ccagggtata gacaatctgc ggtgccggac caaagaaata ccagattgcc
    28501 gcactgacaa gcgcaataac cactactacc ggcacaaata cggctgagat tttatccgcc
    28561 agctgaccga tttctggctt gctgctctgg gcctggcgca ccatacgaat aatccttgat
    28621 agcgtggtat ggctgccaac cgcactggcg cgaaacagaa cgctaccgtc ctgtaccact
    28681 gtcccggcat ggacgctatc gccttcgcct ttttgctgcg ggataggttc gcccgtcagc
    28741 atcgcttcat ccagccatgc ttcgccctgg gtaatctcgc catctactgg cacgcggtcg
    28801 cccgtcgtca ggcgcagtag catgcctggc tgaacttccg ccagcggcac gcttttttca
    28861 ccttcgtcag taaccaggcg cgccgtcggc ggagttaaat cgagtaattt ctccagcgcc
    28921 tttgaagaac gctgacgtgc gcgcgcttcc agcatatgac cgagattaat cagaccgata
    28981 atcatcgcac tggcttcata gtaaagatgc cgcgcttcca tcgggaacca ctgcggccac
    29041 aggttgacgc tcatcgaata gagccacgcc acacccgtac ccagcgccac cagcgtatcc
    29101 atcgtcgccg caccgttcag caggcttttc catgcactgc ggtaaaaatg gccgccggcg
    29161 aaaaccatca ctgccagggt tatcaggccg ataaccaacc acaggctgcg gttgtcagcg
    29221 gtgaccatca tgttatcgcc gatcatcccc cagaccatta ccgggatacc cacagccagt
    29281 gcaacaattg cctgccagcg gaagcgcttc atcgtagcga cggcggtttc ttgctggcgc
    29341 tcgcggcgtt tcgcgtcatc ttcaatcgct tccgcgccgt agcccgcttt ttccaccgcc
    29401 tgcactaaat cttgtgggga ggcactgccc atcaccagcg cagtacgctc cgccaggttt
    29461 acccgtgcct gagtgacgcc cggtacgctt tgcagcgcat tttgtacgcg ggtgacacag
    29521 ctggcgcagc tcatgccgct cagcagcaac tgctggctgt catcgtcatc ggcggtcgct
    29581 gccggaagcg cctcagaaac cgctgtcagt gcttccgacg ggattgatga ctccgccagc
    29641 ggtttagcct ttgggtggct tacagatgcg tcataacccg cttgtttgat ggtttcgatc
    29701 agctgttctg cactggcagt cccggtaacg tgcgcttcag tgatagacac atccgcttgt
    29761 tccacatccg ggcgctgttc gagactttct ttcacgcgtt taacgcagtg accgcaggac
    29821 aggccgtcca gggtcaggtc gatagtttgt gacataaaac actcctttaa gacagttttg
    29881 actggctgtg ataaaggtta aaccttccag caaggggaag gtcaagaaat taataaccca
    29941 ggcgagtaaa agtccgtaaa gattaaaaaa tcggctcgat ttgcatcagg attagacatt
    30001 tatctctttg ttttcctgta gttaagttgc gggtgctaag ttaaatcagg atgcctgaaa
    30061 atcggcaccg gggtgaggaa ttacctcccg catctataaa aaggagttaa caaaagatgt
    30121 tagatgcaaa caaattacag caggcagtgg atcaggctta cacccaattt cactcactta
    30181 acggcggaca aaatgccgat tacattccct ttctggcgaa tgtgcctggt caactggcag
    30241 cagtggctat cgtgaccagc gatggcaacg tctatagtgc gggtgacagt gattaccgct
    30301 ttgcgctgga atccatctcg aaagtctgta cgttagccct tgcgttagaa gatgtcggcc
    30361 cgcaggcggt acaggacaaa attggcgctg acccgaccgg attgcccttt aactcagtta
    30421 tcgccttaga gttgcatggc ggcaaaccgc tgtcgccact ggtaaatgct ggcgctattg
    30481 ccaccaccag cctgattaac gctgaaaatg ccgaacaacg ctggcagcga attttacata
    30541 tccaacagca actggctggt gagctggtag cgctctctga cgaagtcaac cagtcggaac
    30601 aaacaaccaa cttccataac cgggccattg cctggctgct gtactccgcc ggatatctct
    30661 attgtgatgc aatggaagcc tgtgacgtgt acacccgtca gtgctccacg ctcatcaata
    30721 ctgttgaact ggcaacgctt ggcgcgacgc tggcggcggg tggtgtgaat ccgttgacgc
    30781 ataaacgcgt tcttcaggcc gacaacgtgc cgtacattct ggccgaaatg atgatggaag
    30841 ggctgtatgg tcgctccggt gactgggcgt atcgtgttgg tttaccgggc aaaagcggtg
    30901 taggtggcgg tattctggcg gtcgtccctg gagtgatggg aattgccgcg ttctcaccac
    30961 cgctggacga agaaggcaac agtgttcgcg gtcaaaaaat ggtggcatcg gtcgctaagc
    31021 aactcggcta taacgtgttt aagggctgat catgatgaac acggaaggta ataacggtaa
    31081 caaacccctc ggtctatgga acgtcgtttc catcggcatt ggggcaatgg tgggggcggg
    31141 gatcttcgcg ctgctggggc aggctgcgtt gctaatggaa gcctcgacct gggtcgcctt
    31201 tgcttttggc ggtattgtgg cgatgttttc cggttatgcc tatgcgcgtc tgggggcgag
    31261 ctatcccagc aatggcggca ttatcgactt ctttcgtcgc ggattaggca acggcgtctt
    31321 ttcgctggcg ctctcgttac tgtacctgtt gacgctggcg gtgagcatcg ccatggtcgc
    31381 ccgtgctttt ggcgcttatg ccgtgcagtt tttgcatgaa ggcagccagg aggagcacct
    31441 tattttgctc tacgcgttgg ggatcattgc ggtgatgacg cttttcaact ccttaagcaa
    31501 ccatgcggta gggcggctgg aggtgatcct cgtcggcatt aaaatgatga tcctgttatt
    31561 gctgattatt gccggtgtct ggtcgctgca accggcgcat atttccgtct ctgcgccccc
    31621 cagctccggt gcgttcttct cctgtatcgg gataaccttc cttgcctatg cgggctttgg
    31681 catgatggcg aacgcggcgg ataaagtgaa agatccgcag gtcattatgc cacgggcgtt
    31741 tctggtggca attggcgtta ccacgttgct ttatatctcg ctggcgctgg ttttgcttag
    31801 cgatgtatcg gcattagagt tagaaaaata tgccgatacc gccgtagcgc aggctgcttc
    31861 tccgctgctc gggcatgtgg gttatgtgat cgtcgtcatc ggcgctttac tggcgacggc
    31921 ttcagccatc aacgcgaacc tgttcgccgt gtttaacatc atggacaaca tgggcagcga
    31981 acgcgaactg ccgaagctaa tgaataaatc cctgtggcgg cagagtacct ggggcaacat
    32041 cattgtcgtg gtgttgatta tgctgatgac ggcggcacta aatttaggct cactcgccag
    32101 cgttgccagc gccacctttt tgatttgcta tctggcggtg tttgtggtgg cgatccgcct
    32161 gcgtcatgat attcacgcct cgttgccgat tcttatcgta ggtacgttgg tgatgttgtt
    32221 ggtgatcgtt ggctttatct acagcctgtg gtcccagggc agccgtgcgt tgatatggat
    32281 tattggtgca atcttactca gccttattgt ggcaatggtc atgaagcgca ataaaaccgt
    32341 ataacacctc tctgcgcgca gtactgcctg tactgcgcct
//
LOCUS       DBFIFI010000032        32340 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.032, whole genome shotgun sequence.
ACCESSION   DBFIFI010000032 DBFIFI010000000
VERSION     DBFIFI010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.032"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(94..1035)
                     /gene="fabY"
                     /locus_tag="N7B39_001451"
                     /gene_synonym="yiiD"
     CDS             complement(94..1035)
                     /gene="fabY"
                     /locus_tag="N7B39_001451"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HGU2421459.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1080..1517)
                     /gene="dtd"
                     /locus_tag="N7B39_001452"
     CDS             complement(1080..1517)
                     /gene="dtd"
                     /locus_tag="N7B39_001452"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HGU2421460.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(1514..2386)
                     /gene="yihY"
                     /locus_tag="N7B39_001453"
     CDS             complement(1514..2386)
                     /gene="yihY"
                     /locus_tag="N7B39_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HGU2421461.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(2380..2979)
                     /gene="yihX"
                     /locus_tag="N7B39_001454"
     CDS             complement(2380..2979)
                     /gene="yihX"
                     /locus_tag="N7B39_001454"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HGU2421462.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGTWSDLTRIPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3078..3863)
                     /gene="csqR"
                     /locus_tag="N7B39_001455"
     CDS             complement(3078..3863)
                     /gene="csqR"
                     /locus_tag="N7B39_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGU2421463.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEEKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(3897..4793)
                     /gene="yihV"
                     /locus_tag="N7B39_001456"
     CDS             complement(3897..4793)
                     /gene="yihV"
                     /locus_tag="N7B39_001456"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HGU2421464.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDTGNSLLAELESWGVNTRYTKRYKQAKSSQSAIMVDAK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVVLATGGDLAESVRFASGVAASKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            4961..5857
                     /gene="yihU"
                     /locus_tag="N7B39_001457"
     CDS             4961..5857
                     /gene="yihU"
                     /locus_tag="N7B39_001457"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HGU2421465.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRSVLFGENGVCESLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFNMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            5881..6759
                     /gene="yihT"
                     /locus_tag="N7B39_001458"
     CDS             5881..6759
                     /gene="yihT"
                     /locus_tag="N7B39_001458"
                     /EC_number="4.1.2.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HGU2421466.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKTLSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLSASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            6775..8016
                     /gene="yihS"
                     /locus_tag="N7B39_001459"
     CDS             6775..8016
                     /gene="yihS"
                     /locus_tag="N7B39_001459"
                     /EC_number="5.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HGU2421467.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYSLVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWDYCIKYLMDYENGSWWQELDADNKVTTKVWDGKQDIYHLLHCLVIPR
                     IPLAPGLAPAVAAGLLDINAK"
     gene            8083..8985
                     /gene="yihR"
                     /locus_tag="N7B39_001460"
     CDS             8083..8985
                     /gene="yihR"
                     /locus_tag="N7B39_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HGU2421468.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHGSKAAIHGLLAWRDWQI
                     SELTATSVTLTAFLPPSYGYPFMLASQVVYSLNARTGLSVEIASQNIGTVTAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHANPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEITITHPQQALSVSLCSDQPWVQIYSGEKLQRQGLAVEPMSCPPNAFNS
                     GLDLLLLEPGKTHRLFFNIYGQHN"
     gene            9046..9921
                     /locus_tag="N7B39_001461"
     CDS             9046..9921
                     /locus_tag="N7B39_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HGU2421469.1"
                     /translation="MDFFTLFSPDGVMLETNVVCIVIALMFLYTFVGICAGFGGGLTT
                     MPLVTLMLPVKMATPLSVIVGTATALYATWLSRKETDWRSAMVLIIFSFLGIPVGLYA
                     LSYLPDHFMKVGLGGFLILYSFYSMFIPRLPIYDRSWIAAPIGAVAGALGAAFSTNGP
                     PMVIYGMLRNLGPAAFRGTLNAFFTANNIAVIGGLATSGILTVSIFKLVLFCIPTMIL
                     GSLVGQYVHKRISVKVFRVMVFLLLIASGSMLIKGALGIPAAAALLPPFTLLVILQLV
                     FGKKVAAIRQAEASK"
     gene            10029..12065
                     /gene="yihQ"
                     /locus_tag="N7B39_001462"
     CDS             10029..12065
                     /gene="yihQ"
                     /locus_tag="N7B39_001462"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HGU2421470.1"
                     /translation="MDTPRPQLPDFEFHQNNDSFTLHFRQRLILTHSKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRMTSFGKRVMWNWKWNSENYPQLDSRIKQWNKEGVQFLAYI
                     NPYVASDKDLCEEAAKRGYLAKDVAGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGISAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAQTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGETFHGGEITVEAPIGKPPVFYRADSEWAALFASLKNI"
     gene            12111..13496
                     /gene="yihP"
                     /locus_tag="N7B39_001463"
     CDS             12111..13496
                     /gene="yihP"
                     /locus_tag="N7B39_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2421471.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFIGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQQLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPANPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFSGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            13536..14939
                     /gene="yihO"
                     /locus_tag="N7B39_001464"
     CDS             13536..14939
                     /gene="yihO"
                     /locus_tag="N7B39_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2421472.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLSIGTLYLLKFYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFSLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPLTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCI
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEEKTASVTL"
     gene            15006..15698
                     /gene="ompL"
                     /locus_tag="N7B39_001465"
     CDS             15006..15698
                     /gene="ompL"
                     /locus_tag="N7B39_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HGU2421473.1"
                     /translation="MKNINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSIGS
                     GGAVYLDVNYKFTPWFNLTVRNRFNHNNYSSTDLNGDLDNNDSYEIGNYWNFIITDKF
                     SYTFEPHYFYNINDFNSGNGTKHHWEITNTFRYRINEHWLPYIELRWLDRNVEPYHRE
                     QNQIRIGTKYFF"
     gene            complement(15789..17054)
                     /gene="yihN"
                     /locus_tag="N7B39_001466"
     CDS             complement(15789..17054)
                     /gene="yihN"
                     /locus_tag="N7B39_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2421474.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGITIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLKD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(17156..18136)
                     /gene="yihM"
                     /locus_tag="N7B39_001467"
     CDS             complement(17156..18136)
                     /gene="yihM"
                     /locus_tag="N7B39_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HGU2421475.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVREAYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKELLTHLICLGDEEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPEKHLLDERLRKEKEDAINQINHVRNVLQQIKQEASHLLNH"
     gene            complement(18144..18854)
                     /gene="yihL"
                     /locus_tag="N7B39_001468"
     CDS             complement(18144..18854)
                     /gene="yihL"
                     /locus_tag="N7B39_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGU2421476.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(19071..20894)
                     /gene="typA"
                     /locus_tag="N7B39_001469"
     CDS             complement(19071..20894)
                     /gene="typA"
                     /locus_tag="N7B39_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HGU2421477.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            21267..22676
                     /gene="glnA"
                     /locus_tag="N7B39_001470"
     CDS             21267..22676
                     /gene="glnA"
                     /locus_tag="N7B39_001470"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HGU2421478.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            22850..23899
                     /gene="glnL"
                     /locus_tag="N7B39_001471"
     CDS             22850..23899
                     /gene="glnL"
                     /locus_tag="N7B39_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HGU2421479.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            23911..25320
                     /gene="glnG"
                     /locus_tag="N7B39_001472"
     CDS             23911..25320
                     /gene="glnG"
                     /locus_tag="N7B39_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HGU2421480.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            25432..25542
                     /gene="yshB"
                     /locus_tag="N7B39_001473"
     CDS             25432..25542
                     /gene="yshB"
                     /locus_tag="N7B39_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HGU2421481.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(25732..27105)
                     /gene="hemN"
                     /locus_tag="N7B39_001474"
     CDS             complement(25732..27105)
                     /gene="hemN"
                     /locus_tag="N7B39_001474"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HGU2421482.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(27294..27800)
                     /gene="yihI"
                     /locus_tag="N7B39_001475"
     CDS             complement(27294..27800)
                     /gene="yihI"
                     /locus_tag="N7B39_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HGU2421483.1"
                     /translation="MKPSSPSRSKGHAKARRKTREELNQEARDRKRQKKRRGHAPGSR
                     AAGGNNTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELLE
                     TDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQED
                     MMRLLRGN"
     gene            complement(27919..28085)
                     /locus_tag="N7B39_001476"
                     /pseudo
     CDS             complement(27919..28085)
                     /locus_tag="N7B39_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            28382..29014
                     /gene="yihA"
                     /locus_tag="N7B39_001477"
     CDS             28382..29014
                     /gene="yihA"
                     /locus_tag="N7B39_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HGU2421484.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFNEMQPVEETQDGE"
     gene            complement(29396..32182)
                     /gene="polA"
                     /locus_tag="N7B39_001478"
     CDS             complement(29396..32182)
                     /gene="polA"
                     /locus_tag="N7B39_001478"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HGU2421485.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHRDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
BASE COUNT         7811 a         8369 c         8008 g         8152 t
ORIGIN      
        1 aatttataga gttcttattc aatataaccg aaatagccaa ggactaatat aaatgacctg
       61 aatgagagag gcagcaccgc ccctctccat cagctactct tcttcgttcc cgccctcttc
      121 atacgggcca aatggcttcg cgggcagaac gatatacgtg ccttcaaaca ctgcacccgg
      181 cgtctcgtcg ccaaagattt cgacctgcat ctgcacccgt gcttttcgtc cgcgcgccag
      241 acggtcgaga tcgccgctta aggcaccaag gtcggctacc gcatggggtt taccgctaat
      301 cggtttgctg tagcggatat gcgcatccgc cagaataatc gttccgccga ggtggcgttc
      361 gcgcagcatc agccagataa gcccccaacc ggtgagcgtc gccagtgaga ataaactccc
      421 ggcaaacagc gtatggtgcg gattctgatt gccggtttct ggcatggtag tgataaattt
      481 ttgcccggta tattgctgaa tgcgcacgcc cattttttca ctaagcggga tatgttcgta
      541 ccacgcctgt tgcagctgcg cgcaccagtc gccgcgatgc aaaatatcat ccagagtggc
      601 gacgggctta atcatcaaaa aatggcgaat cggcgtggtg gttggcgtgg tgatttctcc
      661 ctgattaaca aaccccagct tggcgaaaaa ctccaccgcg tcttcacggg cgctacaggt
      721 cacgcgctta acgccttcct gacgcgccac cgactccagg gtcatcgcca tcagcgtgcc
      781 taaccctttg tcctgcacgt cgggatgaac ggccataaag cgaatggacg cttcattgtc
      841 ggcattaata tacagtcggc ctaccgccac cagattaccc tgctcgtcga cgaccatctg
      901 gtgatgcgcc atcgcatccc acgcgtcgcg ttccgaacct tttggttgat gcaggggctt
      961 acgcaacatt tcccagcgaa actgatagta acgctctaat tcttcttctg tttgtggaac
     1021 ccgaaggtga tacatagcga tactctctct tgtttcccgt gacaaccctg gaagctggct
     1081 catacctgca accagaatgt cacggggcca tcattgacca gcgatacctg catatccgca
     1141 gcgaagcgtc ctgtttgcgt gttcatctct tgctgacggc agcgttcgac gaaatagtca
     1201 tataacgcct ctgcgcgatc cggtgatgca cctttggaga aacttgggcg catcccccgt
     1261 tcggtatctg cggcgagggt aaactgggaa accaccagca cactgccgcc cgcctgttgc
     1321 acgttgagat tcatcttgcc ttcggcatcg ctaaagatgc ggtagccgag cacacgctcg
     1381 cacagacggt ttgctttctg ttcgtcgtca tccttttcga cacccaataa caccaaaagt
     1441 cccgcaccaa tttcgcccgt cacttctccc tccacggtga cgctggcacg ggttacgcgt
     1501 tgaattaatg caatcatggt tcgtcgtctt cttcttgttc agctgcttgt tttagtttgc
     1561 ggtattcccc gagagtgaca gtaatttccg cgccaagcaa gacgatacac catgtccagt
     1621 agacccaaac aaagagaatg gggatcaccg ccagcacacc gtaaatgagc tgatatgacg
     1681 ggaacatggt gatataaagc gcgaaacctt tctttcctgc ttcgaacagg agtgcagcaa
     1741 caaacgcgcc gacaatcgcg tcgcggttag gtacgcggat ggtaggaaca atgctgtaca
     1801 gcaaccagaa ggagatccac gacaacagca acggaaaaat acgcagcacg ttgtcgatga
     1861 cagtattgag atcgctcgcc cagcgcaggg agagcaaata ggaactgatc gccagactgg
     1921 cccctgccag cagcggcccc agcgttaaaa tcatccagta cacggcaaac gagtaaattt
     1981 tgggtcgcgc tcgtttactg cgccagatgg tattcaacgc gctatcgatg gagtacatca
     2041 acaataacgc ggtgacgatc agcccgcacg ccccaacggc ggtcatcttg ttggaattgg
     2101 caacaaattg ttcgatatac cgctgaataa catcgccagt agcaggcaga aagttggcaa
     2161 aaataaagtg acgtaactga atgctgacgt cggaaaacat gggaaaagcg gcgaaaagcg
     2221 caaaaacaac ggcaaccagc ggcactaatg agagcaacga cacataggca aggttacctg
     2281 ccagggttgt catattgtcc tcatcaatgc gttgccagag tagttttagc caggcccata
     2341 gtggacgggt acggtgcctg gctttgtcct gaatggtttt tagcataaca ccttcgcgaa
     2401 atagtccggg atggtggttt tatctttcac cagaatactg gtaatgccca gctgattagc
     2461 tccttctata ttatcggcgt tatcgtcgaa aaagaccgta tcgctgggtg aaaaaccttc
     2521 cgcctgcaaa acatgctggt aaattcgagc ttcaggtttg cgcatcccca gatcttgcga
     2581 cagatagata tggtcagcag catcacgaat ttccgggtat tcttccggcc agaaggtggt
     2641 atgcaggcgg ttagtattgg aaagcaccac cacgcgatgc ccctgctcac gcagtttatg
     2701 catgatggcg atcacttccg ggcgcagcgc aacaaacacc gcctgccagc cgtgagagaa
     2761 ctgctcgtag cttagcggta gagccatctc atgacacagc gcctctgcga acgcttcgtc
     2821 gctaatttcc ccacgctcat gctgatgaaa cgcctccccc atatgaaaac tcttcttaag
     2881 cgtagccagc ggaatacgcg ttaaatcgct ccaggttccc agcacacggt taaagtcgat
     2941 atcgacaatc acattaccta aatcaaagat atagagcatt tttgcctcct ttcgcgccac
     3001 aagaaattaa ctgtagcggg aaaggatggg tttggctatg tggcaaggta tgaagccagg
     3061 tcaacagtca caaaaatcta cgcgtcttcc tggggaagga tgatttcaat ttggctgtct
     3121 tgtaagcgtg attttagcgc agcgggcggc agctcgtcgg taaagagcgc ggtgacctgt
     3181 gccacgttac caatttcaac cgccgccgaa gcatgatact tagtgtgatc ggcaaccagc
     3241 aggatatttc tcgcgtgcgc catcatcgtt ttcaccacgt tagcttcgtt tacatcaaac
     3301 tccatcaacg cgccatcgct ctcaatcgcc ccaacgcttg ttaccagata atcagcacga
     3361 aaatcggcca caaaggacgc cgctgaaggg ccaatgatcc cgctattatg agagcgcaac
     3421 gtaccgccgg gtaccatcac ttcaaagcgc gggttgtggt aaagaatatg cgccacacgc
     3481 aggctgttgg tgattatccg caaatgatta tggttaagta acgcccgggc aacatgctca
     3541 acggtcgtac caatggtgat aaatattgtt gaaccatcag gaatatagtc tgccaccgct
     3601 tcggcaatcg cttttttttc ctcggtttgc gaaacctcac gctgctcgaa cgccgtatta
     3661 acgacgctgg aagcccgacc cgcgccaccg tgatggcgcg taatcaggcc ttgctcgctt
     3721 aatttacgaa tatcccggcg caccgtctgc gtggagacat ccagcagatt tgccagctca
     3781 tcaatattca tatacccacg ctcggcgatc agcatgagga gttggtcgtg ccgcgggtta
     3841 ccggttagtt cggtaaggct catgaaaatt cctcgaaaaa ccatcaccct ggcattttat
     3901 acaaaaagtg acaaaaaaga tcgggtttga tcacagtcag ggatcccggc tcgtccaccg
     3961 ggacgcgtgc atttcgacgc cgctacaccg ctggcgaagc ggactgactc cgctaaatcc
     4021 ccacctgttg ccagcaccac cgccaatgcg ccgtgaaaaa catcacccgc acctgtggta
     4081 tctaccacat caactttgaa ggccggttga tgttgacgcc cgccattttc cagccagtca
     4141 cagcctgcgc tgccctgggt cacatagaca tgaccatttg tgagcgtttg tgcctgtttt
     4201 agcgcactgg ccatctcttt cacgcccgtt aagcgcgcca gacccggttc tgaaaaggcc
     4261 gcatgatcgc ttaatgccac cagctcgctg atatcctgcg gcgtaatatc accgtccaga
     4321 acggtcatca cgcccgcctg ccgggccaga gtgaaggctt ttttagcgcc gtcgtgccag
     4381 cgtacatctg ccagcacaac atcccactga gagaaatcaa tttcctccaa ccactctgcg
     4441 tcaggcagca ggtccgggct ggggtagtta atgattatcc gctcgccttt ggcatccacc
     4501 atgatggcgg attgcgacga tttcgcctgt ttataccgtt tggtgtagcg ggtgttaacg
     4561 ccccaggatt ccagctctgc cagcaggctg ttgccggtgt cgtcatcacc gacgcgccca
     4621 ataaaatcaa cctgcgctcc cagtcttgcc gccgcaaccg ctgccgtcgc cgctggcccg
     4681 ccaccaactt ccgtataatt tctcgccacg tatttaccgc tctccgtcgg taacccttcc
     4741 acgtaataga tgcgatccat cacggttata cctacacaag caacacgaat catggtcatt
     4801 ccttaaacat tttgggatgc gttcatttta atttcaccaa ttgtttaaaa agtgacgtct
     4861 gtcaattttt tgactataaa ttacaaatac gatcaaaaac agacaatcaa agcgtgtcat
     4921 aaatgacaaa aagtgacatc actgtattca ggagaaggtt atggcagcaa tcgcgtttat
     4981 cggtttagga caaatgggtt cgccaatggc gagcaattta ttgcagcaag ggcaccaact
     5041 tcgcgtcttt gatgtgaatg ccgaggctgt gcggcatctg gtagacaaag gcgcgactcc
     5101 cgccgccaac ccggcgcagg ccgctaaaga tgccgaattt atcattacca tgttgccgaa
     5161 tggcgatctg gtgcgcagcg tgttgttcgg tgaaaacggc gtttgcgaaa gcttatctac
     5221 cgatgcgctg gtcattgata tgtcgaccat ccatccgctg caaaccgata aattgattgc
     5281 cgatatgcaa gccaaaggct tcaacatgat ggatgttccg gtaggccgta cttctgcaaa
     5341 tgccattacc ggtactctgt tactgctggc tggcggcacc gctgaacaag ttgaacgtgc
     5401 cacgccgatc ctgatggcga tgggcagtga gttgatcaac gcaggcggtc cgggcatggg
     5461 gatccgcgtt aagctcatca acaactatat gagcatcgcg ctcaatgcgc tttcggcaga
     5521 agctgccgtt ttgtgcgaag ccctgaatct tcccttcgat gttgccgtca aagtgatgag
     5581 cggtaccgcc gccggtaaag gccacttcac cacttcctgg ccgaacaaag tcctcagcgg
     5641 ggatctttct cccgccttca tgatcgatct tgcccataag gatcttggca tcgcccttga
     5701 tgtcgccaac cagctgcatg tgccaatgcc gctgggggcc gcctcacggg aggtttatag
     5761 ccaggcgcgc gcagcgggtc gcggtcgcca ggactggtcc gccattctgg aacaggtccg
     5821 tgtcagtgcc gggatgactg ccaaagtaaa aatgtaacga ctggataaag gaataaatga
     5881 atgaataagt acaccatcaa cgacattacg cgcgcatcgg gcggttttgc catgctggcg
     5941 gtcgatcagc gcgaagccat gcgcatgatg tttgccgcgg ctggggcacc cgctccggta
     6001 gccgatagcg ttttaactga tttcaaagtt aacgcggcga agaccctctc gccttatgcc
     6061 tcggcgattc tggtagatca acaattctgt taccgccagg tggttgagca aaacgcgatt
     6121 gccaaaagtt gcgccatgat tgtcgccgcc gatgagttca ttcctggcaa cggtattccg
     6181 gtagatagcg tggtcattga ccgcaaaatc aatccgctac agatcaaaca agacggtggc
     6241 aaagccttaa aactgctggt gctgtggcgt agtgatgaag atgcgcagca acgcctggat
     6301 atggtgaaag agtttaacga actgtgccac tcacacggcc tggtaagcat tattgagcca
     6361 gtcgtccgcc caccacgtcg tggcgataaa ttcgatcgcg aacaagcgat catcgatgcc
     6421 gccaaagagc tgggcgacag tggcgctgac ctctacaaag ttgaaatgcc gctttacggt
     6481 aaaggtccgc aacaagagct tctcagtgct tcacaacgtc tgaatgacca tatcaatatg
     6541 ccgtgggtga tcctctcttc cggtgtcgac gaaaaactgt tcccgcgtgc cgtacgcgtg
     6601 gcaatgacag cgggtgcatc gggcttcctg gctggtcgtg ctgtctgggc atcggtcgtc
     6661 ggtttaccag acaacgagct gatgctgcgt gacgtttgcg caccgaaatt acaacaactt
     6721 ggcgatatcg tcgacgaaat gatggctaaa cgccgttaaa atggggatac aaaaatgaaa
     6781 tggtttaaca ctttaagtca caaccgttgg ctggaacagg aaaccgatcg catctttgat
     6841 tttggtaaaa attccgtagt accgaccgga tttggctggt taggcaataa agggcaaatc
     6901 aaagaagaga tgggcaccca tctgtggatc acagctcgta tgttgcacgt ttattccgtt
     6961 gctgcggcga tgggtcgacc tggcgcttac tcgttggttg atcacggtat taaagccatg
     7021 aacggcgcac tgcgcgataa aaaatatggc ggctggtatg cctgtgtgaa tgacgagggc
     7081 gtggtggatg cctccaaaca gggctatcaa catttctttg ctctgctggg tgctgccagc
     7141 gccgtcacaa cgggtcaccc ggaagcgcgc aagctgctcg attacaccat tgaaattatc
     7201 gagaaatact tctggagcga agaagagcag atgtgcctgg agtcctggga cgaagccttc
     7261 agcaaaaccg aagagtaccg cggcggcaat gccaatatgc acgcagtgga agctttctta
     7321 attgtttatg acgtaacgca tgacaaaaaa tggctggatc gcgcgattcg cgtggcttcc
     7381 gtgattatcc acgacgtcgc aagaaataat cattatcgcg ttaacgaaca cttcgatacc
     7441 cagtggaatc cgctgccgga ttacaacaaa gataacccgg cgcaccgctt ccgcgcgttc
     7501 ggaggtacac caggccactg gatcgaatgg ggccgtttaa tgctgcacat ccacgcggcc
     7561 ctggaagccc gttgcgaaca accgccagca tggctgctag aagatgccaa aggtctgttt
     7621 aacgccaccg tgcgtgatgc ctgggcaccc gatggtgcgg acgggattgt ttataccgtt
     7681 gactgggaag gaaaaccggt ggtccgcgaa cgtgtgcgtt ggcctatcgt cgaagcgatg
     7741 ggtacggcct acgcgctcta caccgtcacc ggcgatcgcc agtacgaaac ctggtatcag
     7801 acatggtggg actactgcat caaatacctg atggactacg aaaatggttc atggtggcag
     7861 gagctggatg cggacaataa ggtcaccacc aaagtctggg acggcaaaca ggatatttat
     7921 cacctgctgc attgcctggt gatcccgcgt atcccgttag cgccaggcct ggctccggca
     7981 gttgcggcgg gtctgctgga tattaacgcg aaataactga ctaaaaaaac gacatatcag
     8041 gctgcacgcc gggtatgtcg ttattcaata aaggtacctc gtatgcaaat caccaacatg
     8101 cattgcagtg gacagaccgt ttcacttgcc gcgggggatt atcacgcgac cattgtgacc
     8161 gtcggtgcag gtctggctga attaaccttt cagggctgtc atttagttat tccacataaa
     8221 cccgaagaga tgccgctggc acatctgggc aaagtcctta ttccgtggcc gaaccggatc
     8281 gccaacggct gttatcgcta tcaggggcag gaatatcagt tacccattaa tgaacatggt
     8341 tcaaaagccg ctattcatgg gctgctcgcc tggcgtgact ggcaaatcag cgaattaacc
     8401 gccaccagcg ttaccctgac agcttttctg ccgcccagtt acggctatcc gtttatgctg
     8461 gcttcgcagg ttgtttattc gctcaatgca cgcacgggtc tttccgttga gatcgcaagt
     8521 cagaatattg gtactgtgac cgcaccctat ggtgtgggta ttcatcctta tttaacctgc
     8581 aacttaacat ctgttgatga atatctgttc cagcttccgg caaaccaggt ttatgccgtc
     8641 gatgagcacg ctaatccgac aacgcttcac cacgttgatg agctggattt aaatttcacg
     8701 caggcgaaaa aaatagccgc gacaaaaatc gaccacacct ttaaaaccgc taatgatctg
     8761 tgggaaataa ccattaccca tccacaacag gcgctttcgg ttagcctttg ttccgatcaa
     8821 ccctgggtac aaatttatag cggtgaaaaa ttacagcgtc agggactggc agttgaacct
     8881 atgagttgcc cgcctaacgc ctttaattca ggtctcgact tattattact ggaaccaggg
     8941 aaaacgcacc gcctgttttt taatatttac gggcaacaca attaatttta ttaagaaaag
     9001 aacgagatca ttgtttagag ttgtatttac acaacaggaa atattatgga cttttttacg
     9061 ttattttcac cggatggggt catgctggaa acaaacgtgg tatgtatcgt tatcgcgtta
     9121 atgtttcttt atacctttgt ggggatatgc gcgggatttg gcggtggttt aaccactatg
     9181 ccgctcgtca cgttaatgct ccccgtaaaa atggcaacac cactctcggt aattgtcggc
     9241 accgcgaccg ccttgtacgc cacctggctg tcacgtaaag aaacggattg gcgatcggca
     9301 atggtactga ttattttctc gtttctggga atacccgtcg gtttatatgc actgtcgtat
     9361 ttacccgacc attttatgaa agtgggctta ggcggattcc ttatcctgta ctccttttac
     9421 agtatgttta ttccacgcct gccgatttat gaccgtagct ggatcgccgc acctatcggt
     9481 gcagtagcag gcgcattagg cgcagctttc tcaaccaacg gaccaccgat ggttatctac
     9541 ggtatgctgc gtaacttagg cccggcggca tttcgtggca ctctgaacgc tttcttcacc
     9601 gcaaacaaca tcgcggtgat cggcgggctg gcgaccagcg gaattttgac tgtttcgatc
     9661 tttaaactgg tgctgttttg tattcccacc atgatcctcg gttcactggt tggacaatat
     9721 gtgcataaac gtatttcagt caaagtgttc cgtgtgatgg tctttttatt gctgattgcc
     9781 tccggttcca tgttgataaa aggcgcgctg ggaattcccg ccgctgccgc acttctacct
     9841 ccgtttacct tgttagttat actgcaactg gtatttggta aaaaagtggc ggcaatccgt
     9901 caggctgaag caagtaaata atcacttatg cgggtttaaa tttatatccg cacacattct
     9961 ttaattaatt aaacctgtaa taacaggatg gcatgaaata ttagctttat actctatgga
    10021 gattatttat ggatacgcca cgcccacaat taccagattt tgaatttcat cagaataacg
    10081 acagttttac cctacatttt cgacaacgtc ttattttaac ccatagcaaa gataatcctt
    10141 gtttatggat tggctcaggt atagcggaca tcgatatgtt ccgcggcaat ttcagcatta
    10201 aagataaact acaggagaaa attgcgctta ccgacgccat cgtcagccag tcaccggatg
    10261 gttggttaat ccatttcagt cgtggttctg acattagcgc cacgctgaat atctctgccg
    10321 acgatcaggg ccgtttattg ctggaactac aaaacgacaa ccttaaccac aaccgcatct
    10381 ggctgcgcct tgccgctcaa ccagaggacc atatctacgg ctgcggcgaa cagttttcct
    10441 acttcgatct gcgcggcaaa ccgttcccgc tatggaccag tgaacaaggc gttggtcgca
    10501 acaaacaaac ctatgtcacc tggcaggccg actgcaaaga aaatgcgggc ggcgactatt
    10561 actggacttt cttcccacag cctacgtttg tcagcacgca gaagtattat tgccatgttg
    10621 ataacagttg ctatatgaac ttcgacttta gtgccccgga ataccatgaa ctggcgctgt
    10681 gggaagacaa agcaacgctg cgttttgaat gtgctgacac atacatctcc ctgctggaaa
    10741 aattaaccgc cctgctggga cgccagccag aactgcccga ctggatttat gacggagtaa
    10801 cgctcggcat tcagggcggg acggaagtgt gccagaagaa actggacacc atgcgtaacg
    10861 cgggcgtgaa ggtcaacggc atctgggcgc aggactggtc cggtattcgt atgacctctt
    10921 ttggcaaacg cgtgatgtgg aactggaagt ggaacagcga aaactacccg caactggatt
    10981 cgcgtatcaa gcagtggaac aaagagggcg tgcagttcct tgcctatatc aacccgtatg
    11041 ttgccagtga taaagatctc tgcgaagagg cggcaaagcg cggttatctg gcaaaagatg
    11101 tcgccggtgg cgactatctg gtggagtttg gcgagttcta cggcggcgtt gtcgatctca
    11161 ccaatccgga agcctacgcc tggttcaagg aagttatcaa aaagaacatg attgaactcg
    11221 gctgcggcgg ctggatggct gacttcggcg agtatctgcc caccgacacg tacctgcata
    11281 acggcatcag cgcggaaatt atgcataacg cctggcccgc gctgtgggcg aaatgtaact
    11341 acgaagccct tgaagaaacg ggcaagctcg gcgagatcct cttctttatg cgcgccggtt
    11401 ctaccggtag ccagaaatac tccaccatga tgtgggcggg tgaccagaac gtggactgga
    11461 gtctggacga tggcctggcg tcggttgtcc cggcggcgct atctctggca atgaccggac
    11521 atggcctgca tcacagcgac attggcggtt ataccaccct gtttgagatg aagcgcagca
    11581 aagagctgct gctgcgctgg tgcgatttca gcgccttcac gccgatgatg cgcacccacg
    11641 aaggtaaccg tcctggcgac aactggcagt ttgacggcga cgcggaaacc atcgcccatt
    11701 tcgcccgtat gaccaccgtc ttcaccaccc tgaaacctta cctgaaagag gccgtcgcgc
    11761 tgaatgcgaa gtccggcctg ccggttatgc gcccgctgtt cctgcattac gaagacgacg
    11821 cgcagaccta ttccctgaaa tatcagtacc tgttaggtcg cgacattctg gttgctccgg
    11881 tacacgaaga aggtcgcagc gactggacgc tctatctgcc agaggataac tgggtccacg
    11941 cctggaccgg agaaaccttc cacggtggag aaataactgt tgaagcgccc atcggcaagc
    12001 cgccggtctt ttatcgcgcc gacagcgaat gggctgcact gttcgcctcg ttaaaaaaca
    12061 tctaatcatg cccgcccgtg gggaaactca cgggcaaatg gagaataacg atgagtcaca
    12121 tcacaacgga agatccggca actttgcgcc tgccctttaa agagaaactc tcttacggta
    12181 tcggcgatct ggcctctaac atcctgctgg atattggtac gctttatctt ttgaagtttt
    12241 ataccgacgt tctggggcta cctggcacct atggcggcat tatctttttg atctcgaaat
    12301 tctttaccgc ctttaccgat atgggaaccg gcatcatgtt ggattcccgg cgtaagattg
    12361 gcccgaaagg taagttccgc cctttcattt tgtacgcgtc attcccggtc accttattgg
    12421 caatcgctaa ctttatcggc acaccgttcg atgtcactgg taaaacggtg atggccacta
    12481 ttctgtttat gctctacggg ctgtttttca gcatgatgaa ctgctcctat ggcgcgatgg
    12541 tgcctgctat taccaaaaac cccaacgagc gcgcatcact ggcggcatgg cgtcagggag
    12601 gcgctacgct gggcctgctg ctgtgcacgg tgggattcgt gccggttatg aatcttatcg
    12661 aaggtaatca gcaacttggc tatatcttcg ccgccacgct gttttcactg ttcggcctgc
    12721 tgtttatgtg gatctgctac tcgggcgtga aagagcgtta tgtcgaaacc caaccagcca
    12781 atccggcgca aaagcctggc ctgctgcaat ctttccgcgc aattgccggt aaccgcccac
    12841 tgttcattct gtgcattgcc aacctctgca ctttaggggc gtttaacgtc aagctcgcca
    12901 tccaggtcta ttacacccag tacgtactta acgatcccat cctgttgtca tatatgggat
    12961 ttttcagcat gggctgtatt ttcatcggtg tgttcctgat gcctggcgca gtcaggcgtt
    13021 ttggtaagaa gaaggtctat atcggcggcc tactgatttg ggtgctgggc gatctgctca
    13081 actatttctt cagcggcggt tcggtcagct tcgtggcgtt ctcctgcctg gcattcttcg
    13141 gctcagcgtt tgttaacagc ctgaactggg cgctggtttc cgacaccgtc gagtacggcg
    13201 agtggcgtac cggcgttcgt tcggaaggaa cggtctacac cggcttcacc ttctttcgca
    13261 aagtgtctca ggcgctggct ggtttcttcc ccggctggat gctgacgcaa atcggttatg
    13321 tgccgaacgt ggcgcaggct gaccacacca ttgaagggtt gcgccagctg atcttcatct
    13381 acccaagcgc actggcggta gtcaccattg tggcgatggg ctgcttctac agcctgaacg
    13441 agaagatgta tgtccgcatt gttgaagaaa tagaagcccg taaacgcacg gcgtaataat
    13501 aaacaatgcc ctgcggggcg ttataaggag tgattatgtc tgaccataat ccactgacat
    13561 taaaactgaa tctgcgggaa aaaatcgcct atggtatggg cgacgttggt tcgaatttaa
    13621 tgcttagcat cggtactctg tatctcctca aattttatac cgatgaatta gggatgccag
    13681 cttactatgg cgggattatc tttctggtgg cgaagttttt taccgcgttt accgatatgc
    13741 tcaccggatt tttactcgat tcgcggaaaa atattgggcc taaaggcaaa tttcgtccct
    13801 ttattttata cgcagcggtt ccggcggcgt taattgcaac gctccagttt atcgccacca
    13861 cttttagttt gccggttaaa acgacgattg ccaccgcgct gtttatgatg tttggacttt
    13921 catacagtct gatgaactgc tcgtatggtg cgatgatccc ggccattact aaaaacccga
    13981 atgagcgcgc gcagctcgcg gcttaccgcc agggtggtgc aaccataggg ctattgattt
    14041 gtaccgtggc gtttattccg ttgcagtcgc ttttttctga ctcaaccgtc ggttatgcct
    14101 gtgcggcact gatgttctcc attggtggct ttatttttat gatgctgtgc tacagaggcg
    14161 tcaaagagca ttatgtggac acagcaccaa ccggacataa agccagtatt ctcaaatctt
    14221 tttgcgcgat atttcggaat ccgccattgc tggttttatg cattgctaac ttgtgtaccc
    14281 tggcggcatt taatatcaaa ctggcgattc aggtctatta cacccagtat gtgctgaatg
    14341 atattaattt attgtcgtgg atgggattct tcagtatggg atgcatcctc gtcggcgtat
    14401 tactggtgcc attaactgta aaatgttttg gtaaaaaaca ggtttattta gctggcatgg
    14461 tgctatgggc ggttggtgat atcctgaatt atttctgggg aagtaactct ttcactttcg
    14521 tcatgttctc ttgtatcgcc ttttttggca cggcctttgt aaacagcctg aactgggcac
    14581 tggtgccaga taccgtagat tacggtgaat ggaaaaccgg tattcgtgcc gaaggttctg
    14641 tttataccgg ttataccttc tttcgtaaaa tttctgccgc acttgctggc ttcttgccag
    14701 gcattatgct gacgcaaatc ggttatgttc ccaacatcgc gcaaagtgat gcgacattac
    14761 agggtttgcg tcagctcatt tttatctggc cttgtgcact ggcaattatt gctgcattga
    14821 cgatgggatt cttttacaca ctcaatgaaa aacggtttgc gttgattatt gaggaaatca
    14881 accaacgcaa aaataaagag atggcgacag aagaaaaaac ggcttccgta acgttataaa
    14941 tgtcaatagc cgctatttcc atcctggtgg gtggcggcct ccctacgttt aaaaatggac
    15001 ttattatgaa aaatattaat gcaataattt tattatcttc tctgacctct gcctcagtat
    15061 tcgctggcgc atacgtcgaa aacagggagg catataatct cgcctccgat cagggcgagg
    15121 ttatgctacg tgtgggttat aacttcgata tgggcgcggg tattatgtta accaatacct
    15181 acacctttca gcgagaagat gaactaaaac atggatataa cgaaatcgaa ggttggtatc
    15241 cgttatttaa accaaccgat aaattaacca tccagcccgg tggcttaatt aatgataaga
    15301 gtatcggttc gggtggtgct gtttatctgg atgttaacta caaatttact ccgtggttta
    15361 acctgacagt gcgcaaccgt tttaaccata ataactacag ctcaaccgat ttaaacggtg
    15421 atctggataa taatgacagc tacgagattg gcaactactg gaactttatt attaccgata
    15481 agttctcata tacctttgag ccgcattatt tttataacat taatgatttc aatagcggca
    15541 atggcacgaa gcatcactgg gaaatcacta acaccttccg ttaccgcatt aacgagcact
    15601 ggctgcccta tattgaattg cgttggttag atcgcaacgt ggaaccgtac caccgcgagc
    15661 aaaaccagat tcgtatcggg acgaagtatt tcttctgatg tgaaactgcc ggatgcggcg
    15721 taaacgcctt atccggccta cggttgatac ggttttcgtt ggccggatta agtacatccg
    15781 gccattaatt aagcttcagc cgccagcatc tctttcttac gttggctaat acgcttaacc
    15841 agcattacgg aaaccaccgc accacagaat gcgaagcagg ccataatgcc aaacacgatt
    15901 ttgtagccaa taatccccgg atttaaatcc agaatgtagc catacagact gaagcagaac
    15961 atcgccgggg cgtaaccaat aaagctaccc aacgccatcg ccgcgcctgt tttattttca
    16021 gcaatttttg cttcgccgat aggtgcaaaa aatacggctc gctgtgtaaa gactatcgcg
    16081 ccaaagccca gcgtacatgc catccctaaa tagaccggca tactttcgtg cggcagcata
    16141 atcaacagta cgagcgcagc ggtactgatg ataaaggtgt agcatagata tttactcggc
    16201 gatttcagga ttttatctga aatcatgcca ccaatcggtc caccaatcat tttcagacag
    16261 tattggttaa tgatgccgta agcccccacc agcgcaacgg gcaatagata gatgtttttc
    16321 aggaatggaa tgaagaatgt caggccacag taaaccgcgt aaacgaagaa gacgttgaaa
    16381 gcgataagcc agatagtttt gtctttcagc accgaggtca tactggtgtt ttgcgatgcc
    16441 ccgtcttctt ttttcacctc aacggacggt gcctcttctt tgtcattcag gacaaagaaa
    16501 atagtaatgc ctacggcaat cacaataagg gaatagaacc agatgcctgc tttaaaacct
    16561 aataagccac tgccaaacca ggtaaatact gccaacgcag aaaatgccac cacggtatcg
    16621 acaatgccac gccctgtttc gaagaagcca aacaaccgac cttgttgttc gctattgccc
    16681 aatcgactta ccgacttgag caagaccggc cagttcatca tgtcgcaagt aacgccaaat
    16741 agcgcccaga caaagaggat gccccaatac cccggcattg tcgtcaaata aacacccagt
    16801 aatcctgtcg caatgagtga gaatgacatg gtgtatctgc gcggtagttt atcggcaaaa
    16861 taaatagaca gaaaaaagcc cactgtggta acaaatgagt ttaccgacat agcattacca
    16921 atttgaccat tggtcaaatg gaaatattcc tgcatcggga tataaaacgc atctttcagc
    16981 gacggtaatt tataaattgt accgccacac agtgttaata gactaaataa cgcccatttc
    17041 tttttcgtga gcataacatc ccctaagcga ttttaaaaat cactgtttta aaggcaataa
    17101 gataccgacc attcttatgg aatggtttta ttaactgcgt atgagttaaa acttgttagt
    17161 ggttcagaag atggctcgcc tcttgtttga tttgttgtag tacgttacgc acatgattta
    17221 tctgattaat tgcatcttct ttttctttac gtaaccgctc atccagcaaa tgcttttctg
    17281 gcaatggtgt ttcactcatc tggcgataag ggatccacgg attatcatct tcgtcttcaa
    17341 agcggaacgc tggagcataa taatcaacct cttcttccag gccatatgcc gtcacctgcg
    17401 gttcttcatc gccgaggcag ataaggtgtg ttaataactc tttgaacggc atatctccct
    17461 gacctgaaat acaggcttta tgacccaggc caccttgttc tttaacgatc aaggcatctt
    17521 tgatatggac ctgggtaata tgcggtgcca tcgtttttaa ggcgtctatg ggatgctcat
    17581 ttgcattgat catattcgca aaatcaaaca gtaaggaaag agactccatc tcactttctt
    17641 tgaccagcga caccaactca tgacttttta aatcttcatg ctgttcgatc gtaaaagtca
    17701 ggccgctatc ctgatacgct tcccgcacat aggcaatgtc gttagcgatg atcgataata
    17761 cgtcgcgcaa attaccttca taacgtggat aaaaacgtac ggacgatgcc ccagttttca
    17821 acgcgatggc gacggcttcg tcaatagatg ccttatctga ggcgctggtt tcaatatgaa
    17881 tatcaaggtt cagacggcgg gctttatccc caaaggcaga gagttcttta tcgtccatat
    17941 taccaagaga aaaacgctcg ccatccagaa catggatttt gacgccacgt agattatttt
    18001 cacttgcgat atcaagtaaa tcagcaggca acactctttc cagccgcata tttaaatgaa
    18061 aggcataagc atgtaaataa agaggaagag tgtcgacacg ttgtagaatc tttcttgcgt
    18121 tatttattgt caccataaaa cacctacaga ttaatgatga ggtctacggc ctcaaatcgc
    18181 caatattcaa tatctatctg gacaatattg ccttgctggt cggcgcgatg cttttcaatt
    18241 aagatcgctg gcatacccgc agaaccgcca agatacttgc tgatatcgcc cggcattctg
    18301 acgggtttga aaatcaattt ttttactacc gtctcttttt ggtagcactt ttcccagact
    18361 gcagaaaatg agtggttttc aagttgttca ataaaacccg gagcaacttc aggattaata
    18421 tatgtttcgt gatagaaaac tttatgtcct tccagcgccc cccagccggt gatgcgatag
    18481 agttcagttg atggtgtcac tgcaatcagt ggcgcgagcg tctcgggaat atcgctggta
    18541 cggtttttct cggtaaaccc ccaggaaggc tctcgccctt gctcaattgc ggcccgctga
    18601 aagctcgccg acagctccgg actgtaatta aatcgcggct gggtcacgaa ccacccctta
    18661 cgatccttac ggaagatttt ggattctgcc tcgaggttca acaacgcctg gcgcagcgtc
    18721 atacgtttaa tgcccagcag ttctcccagc tctctttctg aagggagttt tccacccggc
    18781 gtggtgatgc catctttcaa ccaccgatcc agtttctctt ttgcattttc tacggtggat
    18841 tgattctccg ccattgttta atccttttgg tttaaaccag atgaagcatc ttacgcttac
    18901 gccaaaaaag aaagcaatta acggcgtatg actttgcaga taattgtgac aaagctctct
    18961 attgacgtaa ccacagactt ataagggaga tactcgcatt ggtgtgcgct cacgacaggc
    19021 aaaaaaaaac ctgccatcgc tggcaggttt tttatgacta aaaaacgaaa ttaatcgtct
    19081 ttcggtgcgc ggttggcgcg acgacgatcg ttttccgtca ggtgacgttt acgaatacgg
    19141 atagaggtcg gagtcacttc taccagttcg tcatcatcga tgaactccag agcttgttcc
    19201 agagtcatgc ggataggcgg aaccagaaca acggcttcgt cagtaccgga agcacgcatg
    19261 ttggtcagtt tcttaccggt caggcagttt acagtcaggt cgttagagcg gctatgaata
    19321 ccgataatct gaccttcgta aacttctgca ccgtgaccga ggaacagctt accgcgatcc
    19381 tgcaaaccga acagcgcgaa cgcgaccgct ttaccctgac cgttagagat cagtacgccg
    19441 ttctgacgct gacccacttc acccggacgc acgtcgtcgt agtggctgaa ggtggagtac
    19501 agcagacccg taccggaagt catggtcatg aactcagaac ggaagccaat cagaccacgg
    19561 cttgggatca cgtagtcgag acgtacgcgg cctttaccgt ctggattcat gtttttcagg
    19621 tcgcctttac gttcgcccag cgcctgcatt acagaaccct gatgctgttc ttcaacgtcc
    19681 agagtcacgt tttcatacgg ctcttgttta cgaccgtcga tttcacggaa gataactttc
    19741 ggacgggata ccgccagttc gaaaccttca cgacgcatgt tttcgatcag aacagacagg
    19801 tgcagttcgc cacgaccaga aacgcggaac gcatcggcgt cttcggtttc ttctacgcgc
    19861 agcgcaacgt tgtgtaccag ttctttgttc agacgatcca ggatctgacg agacgttacg
    19921 aacttacctt ctttaccgca gaacggcgag gtgttaacgc agaagaacat agaaacggtc
    19981 ggctcatcaa cggagagtgc cggcagcgct tcaacgtttt gcgtgtcgca aacggtgtca
    20041 gaaatgttca gttcgcccag gcccgtgatc gccacgatat cgccagcttc cgccagatcg
    20101 gtttcgatac gttccagacc gaggtggccc agcactttac caactttcgc gttgcgggtt
    20161 ttgccttcgc tatcgatgat agtaacctgc tggttcggct tcactttacc gcgcttgatg
    20221 cggccaatgc cgataacgcc aacatagctg ttgtaatcga gctgagaaat ctgcatctgg
    20281 aacggaccgt caaggtcaac gtccggcgca ggaacgtggt caacaatcgc ctggtacagc
    20341 ggggtcatgt cttccgccat atcttcgtgg tccagacccg cgataccgtt cagcgcagaa
    20401 gcgtaaacga tcgggaagtc cagctgctcg tcggtcgcgt cgaggttaac gaacagatcg
    20461 aatacctgat ccacaaccca atcaggacgc gcgccagggc ggtcaacttt gttgataaca
    20521 acaatcggct tcaggccgta agcaaacgct tttttggtta cgaagcgcgt ttgcggcatc
    20581 gggccgtcaa atgcgtcaac caccagcagc actgagtcta ccatggacat tacacgttca
    20641 acttcaccac cgaagtcggc gtgccccggg gtatcaacga tgttgatacg gtaatcattc
    20701 catttgatag cggtgttttt cgcgaggatg gtaatcccac gctctttctc caaatcgttg
    20761 gagtccatca cgcgctcttg ggtttcggca cgagagtcga acgtaccgga ttgttggagc
    20821 agcttgtcta ccagggtggt tttaccatgg tctacgtgcg cgatgatggc gatattacgc
    20881 aatttttcga tcacaacttt gcctcaggca ttagaaatag cgcgttattg tacacggatt
    20941 aatcgcacta caaaacagga tcacaaacat cctccgcaaa caagtattgc agagtccctt
    21001 tgtgatcgct ttcacggagc ataaaaaggg ttatccaaag gtcattgcac caatatggtg
    21061 cttaatgttt ccattgaagc actatattgg tgcaacattc acatcatggt gcagcccttt
    21121 tgcacgatgt taagcatgat aacgcctttt aggggcaatt taaaagttgg cacagttttc
    21181 gctttatctt ttttacggcg acacggccaa aataattgca gatttcgtta ccacgacgac
    21241 aatgaccaat ccaggagagt taaagtatgt ccgctgaaca cgtactgacg atgctgaacg
    21301 agcacgaagt gaagtttgtt gatttgcgct tcaccgatac caaaggtaaa gaacagcacg
    21361 tcactatccc tgctcatcag gtgaatgctg aattcttcga agaaggcaaa atgtttgacg
    21421 gctcctcgat tggcggctgg aaaggcatta acgaatccga catggtgctg atgccagacg
    21481 catccaccgc agtgattgac ccgttcttcg ccgactccac cctgattatc cgttgcgaca
    21541 tccttgaacc tggcaccctg caaggctatg accgtgaccc gcgctctatt gcgaagcgcg
    21601 ccgaagatta cctgcgttcc actggcattg ccgacaccgt actgttcggg ccagaacctg
    21661 aattcttcct gttcgatgac atccgtttcg gatcatctat ctccggttcc cacgttgcta
    21721 tcgacgatat cgaaggcgca tggaactcct ccacccaata cgaaggtggt aacaaaggtc
    21781 accgtccggc agtgaaaggc ggttacttcc cggttccgcc ggtagactct gctcaggata
    21841 ttcgttctga aatgtgtctg gtgatggaac agatgggtct ggtggttgaa gcccatcacc
    21901 acgaagtagc gactgctggt cagaacgaag tggctacccg cttcaatacc atgaccaaaa
    21961 aagctgacga aattcagatc tacaaatatg ttgtgcacaa cgtagcgcac cgcttcggta
    22021 aaaccgcgac ctttatgcca aaaccgatgt tcggtgataa cggctccggt atgcactgcc
    22081 acatgtcgct gtctaaaaac ggcgttaacc tgttcgcagg cgacaaatac gcaggtctgt
    22141 ctgagcaggc gctgtactac attggcggcg taatcaaaca cgctaaagcg attaacgccc
    22201 tggcaaaccc gaccaccaac tcttataagc gtctggtccc gggctacgaa gctccggtaa
    22261 tgctggctta ctctgcacgc aaccgttctg cgtctatccg tattccggtg gtttcttctc
    22321 cgaaagcacg tcgtatcgaa gtacgtttcc cggatccggc ggctaacccg tacctgtgct
    22381 ttgctgccct gctgatggcc ggtcttgatg gcatcaagaa caagatccat ccgggcgaag
    22441 ccatggacaa aaacctgtat gacctgccgc cggaagaagc gaaagagatc ccacaggttg
    22501 caggctctct ggaagaagca ctgaacgagc tggatctgga ccgcgagttc ctgaaagccg
    22561 gtggcgtgtt cacggatgaa gcgatcgatg cgtacatcgc tctgcgtcgc gaagaagatg
    22621 accgcgtgcg tatgactccg catccggtag agtttgagct gtactacagc gtctaatagt
    22681 tgaagttgta ctacccggcg caacaacgcc gggatttagt tgccgtggaa actttcagcc
    22741 catctctgca tgggcttttt tctccgtcaa ttctctgatg cttcgcgctt tttatccgta
    22801 aaaagctata atgcactaaa atggtgcaac ctgttcagga gactgcttta tggcaacagg
    22861 cacgcagccc gatgctgggc agatcctcaa ctcgctgatt aacagtattt tgttaatcga
    22921 tgacaacctg gcgatccatt acgccaaccc cgccgcgcaa caactgctcg cccaaagctc
    22981 ccgcaaattg tttggtacgc cgttaccgga actgttgagc tacttctcat taaatattga
    23041 gctgatgcaa gaaagtctgg aggcggggca aggttttacc gataacgaag tgacgctggt
    23101 catcgacggt cgctcgcata tcctttctgt gacggcccag cgtatgccgg acggcatgat
    23161 cctgctggag atggcgccga tggataacca gcgccgctta agccaggaac agctacaaca
    23221 tgctcaacaa gttgctgccc gtgatttagt gcgtggcctg gcacatgaga ttaaaaatcc
    23281 gcttggcggt ttacgtggcg cggcgcagct actcagcaaa gcattacctg acccgtcact
    23341 gctggaatat accaaagtga ttatcgaaca ggcggaccgg ctgcgaaatc tggtcgaccg
    23401 tctgttgggg ccgcagctgc ccggtacgcg cgttaccgaa agtattcaca aagtagctga
    23461 acgcgtggta acgctggtgt cgatggaact gccggacaac gtgcggttga ttcgtgatta
    23521 cgatcccagc ctgccggaac tggcgcacga cccggatcaa attgaacagg tcttgctgaa
    23581 tattgtgcgc aatgcgctac aggcgctggg gccggaaggc ggtgaaatca ttctgcgtac
    23641 ccgcaccgcg tttcaactga ccttacacgg cgaacgctat cggctggcgg cgcggattga
    23701 tgtggaagat aacgggccag gcattccgcc tcatttgcag gatacgctgt tttacccgat
    23761 ggtcagcggc cgcgaaggtg gcaccgggct tggcttatcc atcgctcgta atttgattga
    23821 tcagcattca ggcaaaattg aatttaccag ttggccaggg cataccgagt tctcggttta
    23881 cctgcctatc aggaaataaa ggtgacgttt atgcaacgag ggatagtctg ggtagtcgat
    23941 gacgatagtt ccatccgttg ggtgcttgaa cgtgcgctcg ctggagcggg tttaacctgt
    24001 acaacatttg agaacggcgc ggaggtactg gaggcgctgg cgagcaaaac gccggatgtg
    24061 ctgctttcag atatccgtat gccgggaatg gacgggctgg cactgctcaa gcagattaaa
    24121 cagcgccatc cgatgcttcc ggtcatcatt atgaccgcac attccgatct ggatgctgcc
    24181 gtcagcgcct atcaacaagg ggcgtttgat tatctgccca aaccgtttga tatcgacgaa
    24241 gccgtggcgc tggttgagcg cgccatcagt cattaccagg aacagcagca gccgcgtaat
    24301 gttcagctta acggcccaac gaccgatatc atcggcgaag cgccagccat gcaggacgtg
    24361 ttccgtatta tcggtcggct ttcgcgttct tctattagcg tgctgattaa cggcgaatcc
    24421 ggcaccggta aagaactggt cgctcatgcc ctgcatcgcc acagtccgcg agccaaagcg
    24481 ccatttatcg ctctgaatat ggcggctatc ccgaaggatt tgatcgaatc agaactgttt
    24541 ggtcacgaga aaggcgcgtt taccggcgcg aataccattc gtcaggggcg ttttgaacag
    24601 gctgatggcg gtacattatt tctcgatgaa attggcgata tgccgctgga tgtgcagacg
    24661 cgtttgctgc gcgtgctggc agacggtcag ttttaccgcg ttggcggcta tgcgccggtg
    24721 aaagtggatg tgcggattat cgctgccact caccagaatc tcgaacagcg ggtgcaggaa
    24781 ggtaagttcc gtgaggatct gttccaccgc ctgaacgtta tccgcgttca tctgccgccg
    24841 ctgcgtgagc gtcgggaaga tattccccgc ctggcgcgcc attttttaca ggttgccgcg
    24901 cgagaactgg gcgtagaagc gaagttgctg catccggaaa ccgaagccgc gttgacgcgt
    24961 ctggcgtggc caggcaacgt gcgccagctg gaaaacacct gccgctggct aacggtaatg
    25021 gctgccggac aggaagtgtt gattcaggat ttgcctggcg aactgtttga atcaacggtt
    25081 gcggagagta cttcgcaaat gcaaccggac agttgggcga cgcttcttgc gcagtgggca
    25141 gacagagcgc tacgttccgg tcatcaaaat ctgctttccg aagcgcagcc agagctggag
    25201 cggacgttac tgacgaccgc gttgcgacat acgcaggggc ataaacagga ggcggcgcgg
    25261 ctactcggct ggggccgcaa caccctgacg cgtaagttaa aagagctggg gatggagtga
    25321 ttcacggctt gtgtgtaaag attgattatt gagcgcaaat tgctggtatt ttgcgcttta
    25381 ctgttccgat aagttcagta tgatcttgcc cggaaaacgg ggagagtcat tatgctggaa
    25441 tcaataatta atctggtatc gagtggcgca gttgacagcc acacaccgca aactgctgtt
    25501 gctgccgtgc tgtgtgccgc gatgattggg ctgtttagtt gagtttcaga ttgctgatga
    25561 agtgctcttt gttcatgcca gatgctgcgt gaacgcctta tccggcctac aaaagcaata
    25621 ttttcaacag gttttgtagg cctgataagc gtagcgcatc aggcaatctt gttctcagca
    25681 cattgatggc gctttcgttt tctactttgt aaacgaagcg ccattcacta tttaaatcac
    25741 ccgagagaac tgctgcatcc gcgctttctg gcgcagatag gtatcaaagc acatgcaaat
    25801 gttgcggatc agcaagcgac ctttcgccgt cacctgtatt cccttctcat ccacatccac
    25861 cagcccatct tttgctaacg gggcgagcag cttgagatct tccgcaaagt aatcagcgaa
    25921 gtgcaaatcc cactgtttct caataggggc gtaatccaga cggaagttgc agatgagcga
    25981 cttaatcaca tcgcggcgaa tacagtcatc acgcgttagc gcaataccac gccacagcgc
    26041 attgccttgt tcatccactt gctgatagta ctgcttcaac tctttctggt tctgcgcgta
    26101 gcagtcgcca atcatgctga tggcggaaac gcccatcccc agcagatcgg tatcgccctg
    26161 agtggtgtag ccctggaaat tacgatgcag cacgccttca cgctgggcca ccgccagctc
    26221 gtcatccgga cgggcaaagt gatccatacc gataaactga tagcccgatt gcgtcaggaa
    26281 ggcgatggtt tcctgcagga tatcgagttt ttgctgcgga ctcggcaggt cagcatcttt
    26341 gattttgcgc tgagcagcaa aaatggtcgg cagatgcgca tagttaaaga cgctcagacg
    26401 gtcagggttt agctccgcca cgcgtttcag ggtaaaggcg aaactttccg gcgtctgttt
    26461 cggcaggccg taaatcaggt cgatgttggt ggaggtaaag ccaatctcac gcgcatggtt
    26521 aagcagcgca aagataaact cttcatcctg ctcgcggtta accagacgtt gcacttcttt
    26581 gttgaagtcc tgcacgccca tactcaggcg attaaagccc tcggcgcgta aatgatcgag
    26641 tacatccagt tcgatttccc gcggatcgac ttcgatcgaa atctccgcat cggcattgaa
    26701 ctggaagttt tcgcgcagca gcttcatcag gcggctgatt tgcgctttat taagatacgt
    26761 cggcgttccg ccgccccagt gcagctggct gacgtgacgc ccggcgaaca gcggtgcacg
    26821 atggacgatt tcttgctcca gcgcgtccag atactgatcg gccttgtgct gctggcgagt
    26881 aacaatctta ttgcagccgc agaagtaaca aagcttatgg cagaacggga tatgtacgta
    26941 gagagataat ggacgctcag gatagcgcgc cacggcttgt aaaaacgcct gttcgccgaa
    27001 gtcttctgaa aactccagcg cggtcgggta cgaggtgtat cgtggcccgg aatagttata
    27061 tttctggatc agggccagat cccagtcgat ttgctgtaca gacatgctca ctccttccga
    27121 tggcgtctct ggcggctacg gcgtaccggc ggatgcccgt ttcgggtcat cagccggttg
    27181 cgcagccact tttgtcgccg cgacaaccgc tgtagtttaa cgaataacca caccagataa
    27241 catataaccg gaagggttat cagcagaacg ggaaggccca cggcggcaat ccgttagttg
    27301 ccccgcagca gacgcatcat gtcttcttgc ttctcgtctt cttcctcttc ttcgtcatcg
    27361 tcataagaga ggccgagttt ctgcatcaac tcatcaatac gatccagttt agcatccacc
    27421 caggattgct cttcggcact cagggtttcg cctgcttcca gacgttccag cagcgcatcc
    27481 agacgctcat ccgtttctag taattccaac tccgcctgcg gtgaaagcat aggtttctca
    27541 ctcttcggtt tgtgctgttt ggtgactttt tcagtcacgc ccaatggaat aggggtttta
    27601 ctgccaatac gtggatcttt cggtgcgttc tggcctttgc tgcctgacgt gttgtttccg
    27661 cccgctgcgc ggctgcccgg cgcatgacca cgacgttttt tctgacgctt gcggtcacga
    27721 gcttcctgat tcagctcttc gcgcgttttg cgacgcgctt ttgcatgacc tttgctgcgt
    27781 gatggtgaag atggtttcat aatggttcgt cttttagccg ttttattcag tatagatttg
    27841 cggcggaatc taacagaaag caagcaaaga aaaaaggcga cagattactc tgtcgccttt
    27901 tttcctgact cataaccctt aacgggtctt acaatccttg caggccgcca acacaccctg
    27961 tttttccatt agccggaaca atccgtgttg attccatttc cgtttaatta cgtctccgga
    28021 cgtttgtctt cctgacaatc ctctgtcttc gcctcctggc gctcctgacc ctcatcctga
    28081 gtcattgttc ctgttagcgt cctcgctcta tgggtgctac tttacgcctt tgctttaaac
    28141 aaacaatcaa ccgccatcag tatttgcaca ttttcagagt tttatataaa agcaattaac
    28201 tgatttatat agcttatttg acatgcatcg cagaattttc tctgctattt cgcataaaga
    28261 ctatcggcaa tgtctcacaa agcgtatggc ttttacttac aacgctcacg ccgaaaaatc
    28321 cgccttttat tatggttcag gtataatcgc cgcaaagctt tgactgacgg agacgaccgc
    28381 tttgactaat ttgaattatc aacagacgca ttttgtgatg agtgcgcctg atattcgcca
    28441 cctaccttcc gataccggaa ttgaagtggc ttttgcaggc cgttccaacg caggtaaatc
    28501 cagcgcgctg aacacgctga ctaaccagaa aagcctggct cgtacctcaa aaacccctgg
    28561 acgcacccag cttatcaacc tgtttgaagt ggctgacggc aagcgtctgg ttgacttgcc
    28621 tgggtacggt tatgcggaag tcccggaaga gatgaagcgc aaatggcagc gcgcgctcgg
    28681 cgagtacctc gaaaaacgtc agagcctaca aggtctggta gtgctaatgg atattcgcca
    28741 tccgctgaaa gatttggatc agcagatgat tgagtgggcg gtagacagca atatcgccgt
    28801 gctggtactg ctgaccaaag cggacaaact ggcaagcggc gcacgtaaag cgcaattgaa
    28861 tatggtgcgt gaagcggtgc tggcgtttaa cggtgatgtg caggttgaaa cgttttcttc
    28921 gctgaagaaa caaggcgtgg acaagctgcg gcagaaactg gatacctggt ttaacgagat
    28981 gcagcctgta gaagaaacgc aggacggcga ataattttct taccttaatg cttatgccgg
    29041 atgtggcgta tccggcccat aaattcaata gattacaatt ctaccgttgg cctgataaac
    29101 atggtgcatc aggcattacg gatctttcct ttcgcccaat aaaaaacgcc ccagtcatta
    29161 ctgactgggg cggctaaaat attcagccaa atccgattac gtgaagtaaa aggtctgaaa
    29221 gatagaacat cttacctctg taccctacgc gactaacttt actctttttt gttcagttca
    29281 gaaagcactt tttgtaattt tttttcagtt gttgcatagg aatttctaat agccatcaca
    29341 aaacgtgaca gcttatgttg cttacttacg aaaaaagggc atgttcaggc gaatcttagt
    29401 gcgcctgatc ccagttttcg ccactcccca cttccaccag caacggcaca tccagacggg
    29461 tacagttttc catcagttga tgaatctgct tcgcaacggc atcgacatca tctctatgaa
    29521 cttcaaatac cagttcatcg tgtacctgca tgatcatacg tacacgcggt tgctcagcct
    29581 gtaaccacgc atcaacggca atcatcgccc gtttgataat gtcggcggcg gttccctgca
    29641 ttggcgcgtt aatggctgca cgttcagccg ctgcacgacg agcaccattg ctggatttga
    29701 tatccggcag atacagacgg cgtccgtcca gcgtttcaac gtagccctgc tctttcgcct
    29761 gagcacgggt gcgttccata tactccagca cgccagggta gcgttcgaag taaaggtcca
    29821 tgtacttctg cgcttcttta cgtggaatgt tcaattgccg cgccagaccg aaagcactca
    29881 tgccataaat cagaccaaag ttgatcgctt tcgcgctacg gcgttgctcg ctggtgacgg
    29941 tttccagtgg caaaccaaac acttctgccg ccgttgcccg gtggatatct tttccttccg
    30001 cgaatgcggt cagcaagcct ttgtcacgcg agagatgcgc cataatgcgc agttcaatct
    30061 gcgagtagtc cgctgagaca atcacataat cctctggcgc aataaacgcc tggcggatac
    30121 gacgaccttc ttcgttacgc accggaatgt tttgcaggtt aggatcggtt gacgataaac
    30181 gtcccgttgc agttacagcc tggtgataag aggtatgcac acgcccggtt ttcgggttga
    30241 tcatcagcgg cagcttgtcg gtgtaagtcg atttcagctt cgccagacca cgatactcca
    30301 gaatcacttt tggcaacgga tagtccagcg ccagttcttc cagtacctct tccgacgttg
    30361 acggcgcgcc acccggcgtt ttcttcagcg gtttaatgcc ctgtttttca aacagaatgg
    30421 tttgtaactg cttggtggaa gaaaggttaa attcctcacc tgcaatttca tgcgctttct
    30481 tttccagctc agccagacga agggtgagct cttcagaatg attgtgcagc actttcggat
    30541 cgatcttcac accgttacgt tcaatgcgtg aaagtactgg caccagcggc atttcgatat
    30601 tctcgaagac gttcaacggc cctttgtgtt tttgcagatc cggccacatt ttcagatgca
    30661 actgcaaggt gacatcagca tcttcggcgg cgtaacgtcc ggcctcttcg agggcaatct
    30721 ggttaaaagt cagttgattt ttgcctttac ccgcaatctc ttcaaaagtg atggttttgt
    30781 gcttcaacca acgttccgcg aggctgtcca tatcgtgacg cccggcaacg ctattgagaa
    30841 tgtaggactc cagcatggta tcaaacgcaa tcccacgcag ctcaatgccg taattcgcca
    30901 gaataccgcg atcgtatttc aggttttgcc cgaccttcag cgccttttca tcttccagca
    30961 gcggttttag caactcgagt gcacgctcgc gagagatttg atcgggcgca tcaagataat
    31021 catgagcaac cggaatatat gccgctacgc ctggctcgat agcaaaagaa agcccgacca
    31081 ggttagctga gatgttatca aggctgtcgg tttcggtatc aaatgcaaat accggcgctt
    31141 tttccagctt cgcaatccac gctttcagtg tttcttcatc aaggatggtg acgtagttgt
    31201 cataagaaat caccgttgcc gtcacttctg gtgcttcgtc tgcaacactg gtttcctgtg
    31261 gcttcgcggc tggttttgcc cctttggcct gtaaccattt gcccgcttcg acatcagcag
    31321 tccagcgttt gaactcatac tttttgaaca gccccaacaa ctcttccgct gccggttgct
    31381 gcacttccag ttgttcacag gtcagctcca gttcaacgtc ggttttaatc gtcgccagct
    31441 ggtatgagag ataagcaact tctttgtttt gctcgagctt cgctgccatt gttttcgcgc
    31501 cacggaagct caacccagca attttttctg gctcggcata cagcgtatcc agcccgccaa
    31561 gaccttgcag caatgcctgc gcggtttttt caccgacgcc cggtacgcca ggaatgttat
    31621 cagaggagtc acccatcagc gccaggaaat cgatgatcag ttctggcggc acgccgtact
    31681 tattcaccac ctcttccggt ccgaggatgg tattcgtcat ggtgttgata agcgtaatat
    31741 ttggcgtcac cagctgcgcc atatctttat cgccagtgct gatcagcacc ggacgcccgg
    31801 ctttttcggc ttcgcgcgcc agagtaccga taacgtcgtc cgcttctacg ccagaaaccg
    31861 ccagcagcgg cagtcccatc gctttaacca tcgcgtgcaa gggttcgatt tgtgctcgca
    31921 gatcgtccgg cattggcggg cgatgtgatt tgtaatgttc aaacagttca tcacgaaagg
    31981 tttttccctt ggcgtcaaag accaccgctg catgcgtcgg tttatattgc atgatcagac
    32041 tgcgcagcat gttgaggaca ccatacatcg caccggtcgg ctcgcctgcg ctgttagtca
    32101 gcgggggaaa cgcgtgatat gcgcgataaa gataagatga accatctaca aggataagtg
    32161 gattttgggg gatctgaacc ataatgtccg tgcctgttta tcagattatg ggtaaaggat
    32221 gccacagaca ggataaaaac atgagttttt caccgctttt cgtgaaaatc ttttgtagat
    32281 cttctggatc gctcgcaaat cgtcatgtgg ataactttgt gcataaatta tttacatcgc
//
LOCUS       DBFIFI010000033        32130 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.033, whole genome shotgun sequence.
ACCESSION   DBFIFI010000033 DBFIFI010000000
VERSION     DBFIFI010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.033"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            301..1221
                     /gene="cbpA"
                     /locus_tag="N7B39_001479"
     CDS             301..1221
                     /gene="cbpA"
                     /locus_tag="N7B39_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HGU2421486.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFSRQFQHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            1221..1526
                     /gene="cbpM"
                     /locus_tag="N7B39_001480"
     CDS             1221..1526
                     /gene="cbpM"
                     /locus_tag="N7B39_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HGU2421487.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(1680..2279)
                     /gene="torD"
                     /locus_tag="N7B39_001481"
     CDS             complement(1680..2279)
                     /gene="torD"
                     /locus_tag="N7B39_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HGU2421488.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVDELENRVATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPTDHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LRQWLPEFVARCHQYDSFGFYAALSQLLLVLVEGDHQNR"
     gene            complement(2276..4822)
                     /gene="torA"
                     /locus_tag="N7B39_001482"
     CDS             complement(2276..4822)
                     /gene="torA"
                     /locus_tag="N7B39_001482"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HGU2421489.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTSRRATAAQAAT
                     EAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQTYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWHDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKCNGTVEQVTAFNGPVEMVAQCEYVPASQVKL"
     gene            complement(4822..5994)
                     /gene="torC"
                     /locus_tag="N7B39_001483"
     CDS             complement(4822..5994)
                     /gene="torC"
                     /locus_tag="N7B39_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HGU2421490.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRAGANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEISHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            6124..6816
                     /gene="torR"
                     /locus_tag="N7B39_001484"
     CDS             6124..6816
                     /gene="torR"
                     /locus_tag="N7B39_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HGU2421491.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPYTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            complement(6789..7817)
                     /gene="torT"
                     /locus_tag="N7B39_001485"
     CDS             complement(6789..7817)
                     /gene="torT"
                     /locus_tag="N7B39_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HGU2421492.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            7900..10644
                     /gene="torS"
                     /locus_tag="N7B39_001486"
     CDS             7900..10644
                     /gene="torS"
                     /locus_tag="N7B39_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HGU2421493.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQQRLSQQIVAAADEIARLAQGQAN
                     NAATSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALF
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDVPTALMGDPRRIRQVI
                     TNLLSNALRFTDEGQIVLRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFIQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAIGNAAQALETLQNSEPFAAALVDFDLPDVN
                     GITLARQLARQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQANNDLPLDVSQLNEDAQLMGTEKIHEWLALFTQHALPLLDEIDIARATQDSEKIK
                     RAAHQLKSSCSSLGMRSASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLNKKDLNAI
                     "
     gene            10716..11789
                     /gene="yccM"
                     /locus_tag="N7B39_001487"
     CDS             10716..11789
                     /gene="yccM"
                     /locus_tag="N7B39_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HGU2421494.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWHNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETAGSTIFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGALGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFTVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(11838..11972)
                     /locus_tag="N7B39_001488"
     CDS             complement(11838..11972)
                     /locus_tag="N7B39_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HGU2421495.1"
                     /translation="MADFIAQKIAELNKNTGKEVSEIRFTAREKMTGLESYDVKIKIM
                     "
     gene            complement(12000..12230)
                     /gene="ymcE"
                     /locus_tag="N7B39_001489"
     CDS             complement(12000..12230)
                     /gene="ymcE"
                     /locus_tag="N7B39_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HGU2421496.1"
                     /translation="MHRWISQNNIRLPCGAFFISVLFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            complement(12205..12393)
                     /gene="ymcF"
                     /locus_tag="N7B39_001490"
     CDS             complement(12205..12393)
                     /gene="ymcF"
                     /locus_tag="N7B39_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HGU2421497.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            complement(12404..12616)
                     /gene="cspG"
                     /locus_tag="N7B39_001491"
     CDS             complement(12404..12616)
                     /gene="cspG"
                     /locus_tag="N7B39_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HGU2421498.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            12902..13114
                     /gene="cspH"
                     /locus_tag="N7B39_001492"
     CDS             12902..13114
                     /gene="cspH"
                     /locus_tag="N7B39_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HGU2421499.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            13556..13861
                     /locus_tag="N7B39_001493"
     CDS             13556..13861
                     /locus_tag="N7B39_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2421500.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            13968..14612
                     /gene="gfcB"
                     /locus_tag="N7B39_001494"
     CDS             13968..14612
                     /gene="gfcB"
                     /locus_tag="N7B39_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HGU2421501.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLHIAGRDV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            14609..15355
                     /gene="gfcC"
                     /locus_tag="N7B39_001495"
     CDS             14609..15355
                     /gene="gfcC"
                     /locus_tag="N7B39_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HGU2421502.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPVEN
                     VAQLVTQPQLRDRLWWPGALLTDSAVKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGNYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVIVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            15355..17451
                     /gene="gfcD"
                     /locus_tag="N7B39_001496"
     CDS             15355..17451
                     /gene="gfcD"
                     /locus_tag="N7B39_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HGU2421503.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEPLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            17497..18636
                     /gene="gfcE"
                     /locus_tag="N7B39_001497"
     CDS             17497..18636
                     /gene="gfcE"
                     /locus_tag="N7B39_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HGU2421504.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            18624..19070
                     /gene="etp"
                     /locus_tag="N7B39_001498"
                     /gene_synonym="yccY"
     CDS             18624..19070
                     /gene="etp"
                     /locus_tag="N7B39_001498"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HGU2421505.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            19090..21270
                     /gene="etk"
                     /locus_tag="N7B39_001499"
     CDS             19090..21270
                     /gene="etk"
                     /locus_tag="N7B39_001499"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HGU2421506.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDMVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(21390..22694)
                     /gene="appA"
                     /locus_tag="N7B39_001500"
     CDS             complement(21390..22694)
                     /gene="appA"
                     /locus_tag="N7B39_001500"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HGU2421507.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPEPELKLESVVIVSRHGVR
                     APTKATQLMQDVTPDAWPNWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLTKKGCP
                     QPGQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLD
                     NANVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQAL
                     PSELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNA
                     QFYLLQRTPEVARSRATPLLDLIMIALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANL
                     GGALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSL
                     NTPPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(22774..22866)
                     /gene="cbdX"
                     /locus_tag="N7B39_001501"
     CDS             complement(22774..22866)
                     /gene="cbdX"
                     /locus_tag="N7B39_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HGU2421508.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            complement(22879..24015)
                     /gene="appB"
                     /locus_tag="N7B39_001502"
     CDS             complement(22879..24015)
                     /gene="appB"
                     /locus_tag="N7B39_001502"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HGU2421509.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARDDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLSTKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            complement(24027..25571)
                     /gene="appC"
                     /locus_tag="N7B39_001503"
     CDS             complement(24027..25571)
                     /gene="appC"
                     /locus_tag="N7B39_001503"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HGU2421510.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPVLLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            complement(25705..26562)
                     /gene="hyaF"
                     /locus_tag="N7B39_001504"
     CDS             complement(25705..26562)
                     /gene="hyaF"
                     /locus_tag="N7B39_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HGU2421511.1"
                     /translation="MSETFFHLLGPRTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSQRQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            complement(26559..26957)
                     /gene="hyaE"
                     /locus_tag="N7B39_001505"
     CDS             complement(26559..26957)
                     /gene="hyaE"
                     /locus_tag="N7B39_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HGU2421512.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSEFRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            complement(26954..27541)
                     /gene="hyaD"
                     /locus_tag="N7B39_001506"
     CDS             complement(26954..27541)
                     /gene="hyaD"
                     /locus_tag="N7B39_001506"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HGU2421513.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPESVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQG"
     gene            complement(27538..28245)
                     /gene="hyaC"
                     /locus_tag="N7B39_001507"
     CDS             complement(27538..28245)
                     /gene="hyaC"
                     /locus_tag="N7B39_001507"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HGU2421514.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            complement(28264..30057)
                     /gene="hyaB"
                     /locus_tag="N7B39_001508"
     CDS             complement(28264..30057)
                     /gene="hyaB"
                     /locus_tag="N7B39_001508"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HGU2421515.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFNKLMTNLKNGNLATASTEKWEPTTWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            complement(30054..31172)
                     /gene="hyaA"
                     /locus_tag="N7B39_001509"
     CDS             complement(30054..31172)
                     /gene="hyaA"
                     /locus_tag="N7B39_001509"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HGU2421516.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATSIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            31598..31685
                     /locus_tag="N7B39_001510"
     tRNA            31598..31685
                     /locus_tag="N7B39_001510"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31632..31634,aa:Ser,seq:tga)
BASE COUNT         7832 a         8484 c         8212 g         7602 t
ORIGIN      
        1 taattatttg cagtgcaact aattccatgt atattaccca tatatagcgc ctataaaatt
       61 taataaataa taacgcacta gtaaaactta aagtgcctgt ttcaactatc aaaaatcgct
      121 cacccttttt cacctgttta aaatatgttc agcgccccac cttgaggcga cctcctctcc
      181 gcgatgattt caataacata ttctgtgttg gcatatgaaa ttttgaggat taccctacac
      241 ttataggagt taccttacag gggttccttc aatttgtgtt gatttacgcg agataacgct
      301 atggaattaa aggattatta cgccatcatg ggcgtgaaac cgacggacga tctcaagaca
      361 atcaagaccg cctatcgtcg acttgcccgc aaataccatc ctgatgtcag caaagaaccg
      421 gatgccgaag cccgcttcaa agaggtcgct gaagcctggg aagtgttaag tgatgaacaa
      481 cgtcgcgctg agtatgatca gatgtggcaa catcgcaacg atccgcaatt tagccgtcag
      541 ttccagcatg gcgacggtca gagttttaac gccgaagatt ttgacgatat cttctcgtca
      601 attttcggtc agcatgcccg ccagagccgt caacgccccg ccgcacgcgg ccacgatatt
      661 gaaatcgaag tggcggtatt cctcgaagaa acgcttactg agcataagcg taccatcagc
      721 tataacctgc cggtttataa cgcctttggc atgatcgaac aggaaatccc gaaaacgctg
      781 aatgtgaaga tcccggcagg cgtcggcaat ggtcaacgca tccgcctgaa aggccagggg
      841 acgccgggcg aaaacggcgg tccaaatggc gatttgtggc tggtgattca tattgcgcca
      901 catccgctgt ttgatattgt cggccaggat ctggaaattg tggtgccggt tagcccgtgg
      961 gaagcggcgc tgggtgctaa agtcaccgtt ccaacactga aagaaagcat tttgctgact
     1021 atcccgcctg gcagccaggc cgggcaacga ttgcgcgtta aaggcaaagg tctggtgagc
     1081 aaaaaacaga ccggcgatct gtatgcggta ctgaaaatcg tgatgccgcc gaaaccggat
     1141 gaaaacactg ccgcgctgtg gcagcaactg gcagacgccc agtcgtcttt tgatccacgt
     1201 aaagattggg ggaaagcata atggctaatg ttacggtgac ttttactatt accgaatttt
     1261 gcctgcatac cggcatctct gaagaggaat tgaatgaaat tgtcggtttg ggggtggttg
     1321 aaccgcgtga gatccaggaa acaacctggg tatttgacga ccatgccgcc atcgtggtgc
     1381 aacgcgcggt gcgcctgcgt catgaactgg ctctggactg gccggggatc gcggtggcgc
     1441 tgacgttaat ggatgatatt gcgcacctga agcaggaaaa ccgcctgctg cgccagcggc
     1501 tttcccggtt tgtagctcat ccgtgatgtg tgtttgccgg atgtgatgac gcatcttagc
     1561 ctgcctgcaa agagtacttt tcctgtgggc cggataaggc gtgttcacgc cgcatccggc
     1621 ggcggtgccc taatgcctga tgcgacgcta aacgcgtctt atcaggctaa caaacgacgt
     1681 tatctgtttt ggtggtcgcc ctcaactaac accagcaata actggcttag tgccgcgtaa
     1741 aaaccaaagc tgtcatactg atggcaacgc gcaacaaact ctggtaacca ttgccgcaac
     1801 gccgtgagtg ttttttgccg taaactgtcg attcttcgcg caggaaccgt cccctctccc
     1861 agcgaaaaat gcagatgact caacaactcg agatagatcg ccagatgatc tgtcggttcg
     1921 ttgaaattgc cgctggtttc catccctgcc tcaactaaca agcgtttaat ctcttgctcg
     1981 tcctgtttgt aggccgatgc atacggcagc gccgcttgtt tgtcagtcat cagaaacagg
     2041 ccgcaaaagt cagcggccag ttccagacgg gcatcgtcac gtactgtcag agtggcaaca
     2101 cggttttcca gctcatccac cgccgcagtg agcggcggtt cgcttttcag caacgaaaac
     2161 cattcagcca tctgcgcact ggcgatttgc gtcagttgtt catcgtccag ttcacgggag
     2221 aacaactgcg ccagccaggc gtaaacacag gctatctgtt gtgctgtcag cgtggtcata
     2281 atttcacctg cgacgcggga acatattcgc actgcgccac catctccacg gggccgttaa
     2341 acgccgttac ctgctccact gttccgttgc acttctcaat ttccaccagc gtagtgtgcg
     2401 cactggtcgc ctgcgccagc tgcgaagtac cgatgtcaat ggtcaacacg ttggggttac
     2461 cgtatttgca cagcgcacca ggctcgccgc ctttatctgg atcgtgccat gccccttcgt
     2521 gaattcgcgc cacgccgggt gcatagcggt cagaaactac tgcccctgcc aacacctgac
     2581 cgcgagcgtt aaagacgcgt accacatcac cgttacgaat accgcgcgcg ctggcatcct
     2641 gcgggttaat gaacactggc tctttacccg ctaccgtata ttgctgacgc agagtttccg
     2701 actcacataa ctgcgagtga agtcggaaat ccggatgcac agattgcaga tgcaacggat
     2761 acgtctgcga gccgggccca ccgtgggagc gttcgatttt ctcaaaccac atcggatgcc
     2821 cctgacaatc gtcgtagttc atatcggcga tggttttcga atagatctca atcaggccac
     2881 tcggcgtgcc cagcggttcg agatctggat cttcgcggaa tgcctggtgg cgaacaaaca
     2941 tctgcggatg gtcaaactcg acgtactctt tgttattcca gaagtcatca aacgctggca
     3001 aatgaacgcc gcgtcctttg ccttgctgaa caccttcctg ccagatgcgt ttcagccagc
     3061 ccatttcgtc cagcccttcg gtaaaggctt cttcgcgatt aaagcggcgg cacagctcgc
     3121 ggaaaatatc aaagtcgttg cgcgcctcga actgcggcgg cacgacctgt ttcatggcga
     3181 taatgccacg gttggagtgg ttgccgtact ggtcgagatc gttacgctca aactgcgtgg
     3241 tcgcaggcag cacgatatcg gcaaagcggc aggttgaggt ccactggtta tctatggcga
     3301 taaccgtttc cagtttgcgc cagccttcaa taatgcggtt gatctgctga tggcgatgga
     3361 aggggttagt tccggcaaaa atacacattt tcagcggcgg cagttttacc gatttaccgt
     3421 tccagttgat tactttcccc ggttcgagga tcgcatcgat aaaacgggca atcggaatgg
     3481 tgctgctgta gcctttgtaa tcactgttgt cgtgaacagg cggaatcgac gtagagccgg
     3541 agaaaccact cagaataacg cctttacgcc ccggcgtgcc tgcgccgtta tagtgccagc
     3601 caaaaccaaa gccaccacct ggcaggccaa tttgccccag catcgccgcc agaaccacaa
     3661 tcatccacgc ccactgttca ccgtgctgca tacgctgtac gcaccagcca gcaataatct
     3721 gcgttctgtt cgccgccatc tgccgcgcca gtccacgaat ggtttcggca tcaatgccgg
     3781 tcagtttttc agcccatgcg gcatctttcg gctgaccgtc tttctcaccc agcagatacg
     3841 gcaggaactg ctcaaaaccc acacagtagt tagcgaggaa gtttttgtcg tacaggtttt
     3901 cactgtacaa cgtatgcgcc agcgccagtt gcagcggcac gtcagtttgc gggttaaccg
     3961 caatgtgctt cacatgctcg cgccccagat actcatgggt ggatgtaaca accggatcga
     4021 tgctaatgac ctcaatttca ccggcggcga ctttcgcttt caactgcgcg taatattcat
     4081 aaacatcgtg atccgggcac caccagttcg cttgctggtt tttcagcaaa tcggagcccc
     4141 acagcacaat ggttttgctg ttctgcaata ccagcggcca ggaggtttgc tgttcataca
     4201 cttccatcga accgactacg cgcggcagga tcacctgcgc agcaccagta gagtaatctc
     4261 cgcccgtacc aacgctatta ccatgcaagg caatagcttt cgccagcatc cccgaagcgt
     4321 tatggaacat ccccgtcgat tgccaaccac tggcggtcag caaggcactc ggcccgtgag
     4381 ttttctgtac gcgttccagt tcttcataga acatgtcgag ggcttcatcc cagctcacac
     4441 gcacaaaacg gttatcaccg cgctgggagg tgtcgctcag atggcgctta cgcagccagt
     4501 ccacgcgtac catcggataa cgaatacgcg ccgcgttgtg tacatgatcc ggcaatccgg
     4561 caatcatttt cgacggatat ttatccagtt cgaacggttt tgccgccaca aagcgaccat
     4621 ccttcaccgt cgcgcggata gccccccagt gcgacccggt aagaatgccc tctttcgaga
     4681 tgacagcctc agtcgccgct tgcgccgcag tcgcacggcg cgacgttaac aatgacggcc
     4741 ccagcatccc ggcgacggtt aagccgccga gttgtgccag aaaacgccga cgtgatgcct
     4801 gaaagagatc gttattgttc attatttttc ttccttctta tcgccgtgag ccttacctgc
     4861 ggtgtcagac gcattcattt gcagatattt caacaaggtg cgttcttcac gtttatcgag
     4921 gctggtaaag ccgatcatgc cgttgagcgt gccaatccag ccgttagcgt caaagtgaga
     4981 gatttccggt gcgccgtggc actggttaca ggtgccgttg tacaacgaat ccgcataagc
     5041 ccagatcggt ttgatatcgt tcaccatgtc acctttcttc atccacgcgg tggcctgcaa
     5101 cttgctccac tcggtattgg tgtcggcaac ggtggttttc tccagcgttt ttacctgctg
     5161 ctgcacatca ccacgaatcg aggcaacaaa gatgcgttta cctgggaatt gggtgagtac
     5221 acgctgacgt ccggcgcttt ctgtccagcc ggtaatttca atttgcagcc agtcgccgtc
     5281 acgtttaagg actttcactt ccgaggcagg cagcagagaa ccagaggctt ctttatcgcc
     5341 tttcgccgca taaattggct taatatcaat agagtacagc gtgtcaccac tgtcattagc
     5401 accggcgcgc agctcatcga actgcttacg gaagccgcta ctcatatccg gtaactggtg
     5461 ggcaatacct ttatgacagt cgatgcagga ttgattatct ttcgctgcca ccttcatctg
     5521 acgtgccgct tcaggatgct gcttcgcatg atccatcgca tcgtagttat ggcaggagcg
     5581 acaggttgcc gagttgtttt ctttcattcg cgcccattca cgctcggcaa gttccgcgcg
     5641 tttggcttca aatttttcag gtgtatcaat ggagtgggca ataaaggtct ggtagatatc
     5701 gttgctcgct tccagtttgc gcttcaccat gcctggaata tccggcggga tatgacagtc
     5761 atggcattca gctcgcacgc cggaggcgtt ctggaaatgc accgactgtt tatattcttc
     5821 atacactggt tgcatactgt ggcaactgac acaaaattcg gttgtgctgg tgactttgat
     5881 cccaacgtgt ggcaatacaa tcagcgcaat gccaatcaca atcccaatag cgaccagcgc
     5941 cagtaccgac caacgagcac tgggtcggcg tagcgcgttc cagagtttcc gcataatagc
     6001 ccctgtaaaa ttatggttta gtgaagcgat cttaatgagt aaatatgaac agcggcactg
     6061 gtcaggatga acggcttacg gcagaatatg aacagatatg aacagaatga gtaaaaccct
     6121 ctgatgccac atcacattgt tattgttgaa gatgagccgg ttacccaggc gcgattacaa
     6181 tcctacttca ctcaggaggg gtataccgtt tccgttacgg cgagcggtgc cgggctacgg
     6241 gaaattatgc agaatcagcc ggtggattta attctgctgg atatcaactt gcccgatgaa
     6301 aatggcctga tgttaacccg cgccctgcga gaacgctcaa cggtggggat tattctggtt
     6361 accggacgca gcgatcggat tgaccgtatt gttgggctgg aaatgggcgc agacgattac
     6421 gtcaccaaac cgctggaact gcgcgaactg gtagtacggg taaaaaatct gctctggcga
     6481 atcgacctcg cgcgacaagc tcaaccgtac actcaggaca actgctatcg ctttgcgggt
     6541 tattgcctga atgtgtcgcg ccatacgctg gagcgggatg gtgagccgat taaactgacc
     6601 cgcgcagagt atgaaatgtt ggtggcattt gtgaccaatc cgggcgaaat tctcagccgt
     6661 gaacgtctgc tgcgtatgct ctctgcgcgt cgggtggaaa accctgacct gcgcaccgtc
     6721 gatgtgttaa ttcgtcgttt acgccataaa ctcagcgcgg atttactggt gacgcaacat
     6781 ggcgaaggtt atttcttagc cgctgatgtg tgctgataaa aatagaccgg acgaaatccc
     6841 ccaggcgaca gcgagcggcg gatatgttcg cggtcggcat ttttcggcgt cagaactaaa
     6901 atcggtgggc tgacattatc agacaccgat tgcccctgta attgcctgat ggcctgctca
     6961 actgccagtt ccccctgcca gaccatttga tcgctggcag ccataatcac tcttccccgc
     7021 ttcagcccgc gatacacctg atgtgaaaga taaaacgaca ccacggtaag cggcgttttc
     7081 aggttacgcc cttcccccat tgccgcctct gccgcaatgg ccgttccggc aacgacgtcg
     7141 atttctggat ggcgttccag catctcctgc aacaggttac gctggatttc aatatcgtta
     7201 tcaccaagcg caatatcaac aatacgcacc gggcttccgg caatggctgc gcgaaaaccc
     7261 tcgaccatct ccttactgcc cccggcgtta tcgggtccgg gcatcaacag cacattcagt
     7321 ggtttaccgt gcgcccattg caccagatat cgcccaggtt gatagcccat ctgaaaccag
     7381 ggcacaccaa cgcggctttt cacctgggga gcatcaatag catttaccag ctcgatcacc
     7441 ggcagatttg ctacttgctt ttgcagatcg ggaaatgacg tcgtgctact accgagcaaa
     7501 atggcctctg cgccccactg tttacactgg tcgatttgtg cttgctgggt agccaactgg
     7561 ctgtagccgc ctgcctccag cacttttaaa tccacaccgt agcggcgagc agcctcctgc
     7621 ataccatagt tcaacgataa ccaatatgaa tctttcaggc tgggataaag cgcgcacagt
     7681 ttccatacgc gtttggcttt tagcggcata gaggcttgca ccgtgaaatg ctgcgcatca
     7741 tgccagcgca acaggttatc agccgaaaat gccggcaaca tgaaaaggga aagaagtaaa
     7801 aatagcagca cgcgcatgat agcctcatca ataataaggc tttatgctag atgcattccg
     7861 ctttgcgact caacattttt caccttaagt gcaccgaccg tgaatttaac cctgacccga
     7921 agactctgga tgggctttgc cctgatggcg ctgttaaccc tgaccagtac cctggtggga
     7981 tggtacaacc tgcgctttat cagccaggtg gaaaaagaca atactcaggc attgattcct
     8041 accatgaata tggcgcgcca gttgagcgaa gccagcgcct gggaactttt cgccgcgcag
     8101 aacctgacca gtgccgataa cgaaaagatg tggcaggcgc aggggcgaat gctcaccgca
     8161 caaagcctga agattaatgc gttgctgcaa gcgttacggg aacaaggttt tgataccacc
     8221 gctattgaac aacaggagca ggagatctcc cgttcgttac gtcagcaagg ggaactggtg
     8281 gggcggcgtc tgcaactgcg ccagcaacaa cagcgactca gtcagcagat agtcgctgcc
     8341 gccgatgaga tcgcacgcct ggcgcaaggt caggcgaata atgcggcaac ctccgccgga
     8401 gcgacccagg ccgggattta cgatttgatc gaacaagatc agcgtcaggc tgctgaaagt
     8461 gcactcgatc ggctgattga tatcgatctt gagtatgtta accagatgaa tgaactgcgc
     8521 cttagcgctc tgcgggtgca gcaaatggtg atgaatctgg ggctggagca gatccagaaa
     8581 aatgcgccaa cgctggaaaa gcagctcaat aatgcggtga aaattctgca acgtcggcaa
     8641 atacgcattg aagatccggg agttcgtgct caggtcgcaa caacgttaac taccgttagc
     8701 caatatagcg atttgctggc gctgttccag caggacagtg aaatcagcaa tcacctacaa
     8761 actctcgcac aaaataacat cgcccagttc gcgcagttta gcagcgaagt cagtcagctg
     8821 gtcgacacca ttgagctgcg taatcagcac ggactggcgc atctggaaaa agccagtgca
     8881 cgcgggcaat acagcctgtt attgctgggg atggtttcac tttgcgcact gattctgatc
     8941 ctctggcgcg tggtttatcg ctcagtcacg cgtccacttg ccgaacaaac gcaggcgctg
     9001 caacggctgc tggacggtga tattgactcc cctttcccgg aaaccgctgg cgtacgggag
     9061 ctggacacca tcgggcggct gatggatgcg tttcgcagca gtgttcatgc actgaatcgc
     9121 caccgcgaac agctggcggc gcaggtcaaa gcgcgtacag ctgaattgca ggaactggtg
     9181 atagaacacc gacaagcacg ggcggaagca gaaaaagcca gccaggcaaa atcggcgttt
     9241 ctggcggcga tgagccatga gatccgcaca ccgctgtacg gtattctcgg caccgcacag
     9301 ctactggcag ataaccccgc acttaacgcc cagcgtgatg atttgcgggc aattaccgac
     9361 tccggcgaat cgttgctgac catcctcaac gatattctcg attattccgc tatcgaagca
     9421 ggtggcaaga atgtttcggt cagcgatgaa ccctttgaac cgcgaccgct gctggaaagt
     9481 accctgcaat taatgagtgg acgggtgaaa ggtcgcccga ttcgcctggc aacggcaatt
     9541 gccgacgatg taccgactgc gttaatgggc gatccgcggc gtattcgtca ggtcattacc
     9601 aacctgttga gcaacgccct gcgttttact gacgaagggc agatcgtttt acgtagccgt
     9661 actgatggtg agcaatggct ggtcgaagtg gaagacagcg gctgcggtat tgatcccgcg
     9721 aaactggcag aaatcttcca gccatttatt caggtaagtg gcaaacgcgg cggcaccggg
     9781 ctggggctga ctatcagtag ccgtctggcc caggcgatgg gcggcgaact gagcgccacc
     9841 agcacgccgg aggttggaag ctgtttttgt ttacgcttgc cgttacgtgt tgccacggca
     9901 cccgtgccaa aaacagtcaa tcaggcggtg cgtcttgacg gtttacgttt gctgttaatt
     9961 gaagataacc cgctaaccca gcgaattacc gttgagatgc tgaacaccag tggtgcgcag
    10021 gttgttgcta ttggcaatgc cgcgcaggct ttagagacac tgcaaaatag cgaaccgttt
    10081 gctgccgcac tggtggattt tgatctgccg gatgtcaacg gcattacgct tgcccgacaa
    10141 ctggcacgac aatatccgtc gctggttttg attggcttta gcgcccatgt cattgacgaa
    10201 acgctgcgcc agcgtaccag ttcactattt cgcgggatta tccctaaacc ggtgccgcgt
    10261 gaagtgctcg gtcagttact ggcgcactat ctccaactgc aagccaataa cgatctacca
    10321 ctggatgtat cgcaactcaa tgaagacgct cagttaatgg ggacggagaa gatccacgaa
    10381 tggctggcat tatttacaca acatgccctg ccgcttctcg atgaaatcga cattgcccgc
    10441 gccactcagg atagcgagaa aataaagcgt gccgcgcatc agctaaaaag cagttgctcg
    10501 agtctgggaa tgcgtagcgc cagccagctc tgcgcacaac tggagcagca gccattatct
    10561 gccccccttc cacacgaaga aattacacgc agtgttgccg ctctggaagc atggttgaat
    10621 aagaaagacc tgaacgcgat ttgaatcaat atcagccagt taagaataac tagaatagtg
    10681 gcatctggta tgcaacttaa cggactggca aaatcatggc agagaaaaaa agaacccgct
    10741 ggcagcggcg gccaggcacg acgggcggca aattaccgtg gaatgactgg cacaatgcca
    10801 cgacctggcg taaagcgacg caattattgc tgttggcaat gaatatttat attgccatca
    10861 cgttctggta ttgggtgcgc tattacgaaa cggcaggtag tacgatattt gtcgccagac
    10921 cgggaggtat cgaaggctgg ctaccgattg ccggtctgat gaatctgaaa tatagccttg
    10981 caacaggtca gttaccgtcc gtccacgccg ccgcgatgct gttattggtc gcttttatcg
    11041 tcatcagtct attactcaaa aaggcctttt gctcatggtt atgcccggtt ggtacgcttt
    11101 ctgaattaat cggcgctctc ggtaacaaac tgtttggtcg gcaatgtgtc cttccccgct
    11161 ggctggatat tcctctgcgc ggtgtaaagt atttgctgtt gagttttttt ctctatatcg
    11221 cgttattgat gcctgctcag gcgattcact attttatgtt gtcgccctac agcgtggtga
    11281 tggacgttaa aatgctcgat ttctttcgtc atatggggac cgcgacatta atcagcgtga
    11341 ccgttttgct gattgccagc ctgtttattc gccatgcctg gtgtcgttat ctttgcccat
    11401 atggcgcgct gatgggcgtg gtttcgctat tatcgccgtt taagattcgt cgcaatgccg
    11461 aaagttgtat cgattgtggc aaatgcgcaa aaaattgccc atcacggatc ccggtcgata
    11521 aattaattca ggtacgaaca gtggaatgta ccggctgtat gacttgcgta gagtcatgtc
    11581 cggtagcctc aacattgacc ttttcactgc aaaaacctgc ggcaaataaa aaagcctttg
    11641 cgttgtctgg ctggttaatg acgctactgg ttctggggat tatgtttacg gtgattggtt
    11701 acgcaatgta tgcaggagta tggcaaagcc cggtgccgga ggaattgtac cgacgcttaa
    11761 ttccgcaagc gccaatgatt ggtcactaaa ttagcaacaa gcggaacatc acctcgcacg
    11821 gggtgatgtt cacaaaatca cattattttg attttgacat cataactttc aagcccggtc
    11881 attttttctc gcgcggtgaa gcgaatttca gagacttctt tccctgtatt cttgttcagc
    11941 tcggcgattt tttgcgcgat aaagtcggcg atttccgttt cggcttgttt tttaactctt
    12001 caatattcac tgttaacctc ttctggtctt aaaagacagc aacacgccag taaggtgttg
    12061 gttcccggta ccctggtcag gtaagaaatg ttataagcca tctccccgaa agattcaata
    12121 atcaacaggt tatcgcttac tatacataca gcgttgaaaa aaaataagac agatataaaa
    12181 aaggctccac aagggagcct gatattattt tgtgaaatcc agcggtgcat ggtcagtgcg
    12241 tatttgcagc gcgacgttat caaaggtgat catcgtcgat ttacaaaaaa tacacttagc
    12301 gcccaaaggg tttcgctccg tcacatcgaa tgccgatgtg cggtactgtg aaccatgaca
    12361 acatggacag cgaaaatgga gatgttgagt aataacggca accttagagc gtaactacgt
    12421 tcgctgccgc ggggccacgt tgaccctgct caatagaaaa ttcaactttc tgattttcgt
    12481 tcagcgtgcg gaattcattg ctctggatgg cggtgaaatg gacgaaaacg tctttgctgc
    12541 catcatcagg agtgataaag ccaaaacctt tatctgcgtt aaaccatttt actaaaccag
    12601 tcattttatt agacatattt tacttccttc attttgggcc tttcggcaaa cagggcttta
    12661 atacagaaac tacttagtgt tcaggagaga gactcaaaag aaggggataa tgcctgataa
    12721 tgagaactgc tttagtaaac tactttgtat gctgtctgtc tttccaaccg acgcagctat
    12781 taacgcatga taattgagat taagcaaatt ttattttagc cgtccggcgg tcttaaatat
    12841 cgctggcttt attttattaa ccccgtatag tgcagcgcag tattttcagt aaggaatttg
    12901 tttgtcccgt aaaatgacag gaattgtcaa aacctttgat cgcaaaagcg gcaaaggatt
    12961 cattatcccc tccgacggtc gtaaagaagt tcaggtccat atttccgcat tcactccccg
    13021 cgacgcagaa gtgcttatac caggattacg cgtggaattt tgccgcgtaa atggcctgcg
    13081 aggaccaaca gcggcaaatg tttatctctc ttaatatgac gctgcttgtt taaagctgac
    13141 tggttttatt ggcatagttc aatagtcaac accatgtatt ataacacaag ttactttttc
    13201 accggaaaac taatccattt aaccgaatat ctctacaact aatagttata aatgccactt
    13261 aacagcgagt tatagtacca cttgaaacta tattgccaac aatagttaat agctttctta
    13321 acaattttct tatctttaat ttgtattttg ctaagattta tcttatgctc acaggtcgta
    13381 tttccagagg ggaatagtta acaatcttat atttccctct gcgcgccttt ctcacccgct
    13441 gtatatagtg caagtggtat gaggaaaggg agatttcaca aggatgttat actcaaagta
    13501 gacaatcctg atctcaagcg tacgtattgt ccatgcgatg aataaaggaa aagttatgaa
    13561 acacaagctt tctgcaatcc ttatggcctt catgttgacg acgccagctg cgtttgctgc
    13621 ccctgaagca gcaaacggga ccgaggcaac gacgggtact actggcacca caacgaccac
    13681 taccggtgca accacgacgg ctgctaccac tggtggcgtg gctgctggcg ctgtcggcac
    13741 ggcgacagtt gtcggtgtgg caacggctgt tggtgtggcg acgttggcag tagttgcagc
    13801 aaatgacagt ggcgatggcg gttctcataa cacctcaacc accacgtcca ctacgcgcta
    13861 attctgctta aacgcgtcga aattaaagtt aaataatacc cggttgcgat attcgtaccg
    13921 ggctttagag atttgcattt ttttaattaa cctttcaagg attaaaggtg cgccctctta
    13981 ttttatcgat tttcgcacta tttcttgcgg gatgtacgca cagccagcaa agtatggtcg
    14041 atacatttcg cgccagcctt ttcgataatc aggatatcac cgtagcggat cagcagatcc
    14101 aggcgttgcc ttattccacg atgtatttac gccttaatga agggcaacga atctttgtgg
    14161 tactgggata tatagaacaa gaacaaagca aatggttatc ccaggataac gccatgctgg
    14221 ttacccacaa tggacgtctt ttaaagaccg tcaaacttaa taataatctg ctggaagtga
    14281 caaattccgg gcaggaccct ctgcgtaacg cgctggcaat aaaagacggc agccgctgga
    14341 cgcgcgatat tctctggagt gaagacaacc attttcgctc tgcgaccctg agttccactt
    14401 tttcctttgc tggattagag acgctgcata ttgcgggtcg cgatgtgctg tgtaatgtct
    14461 ggcaggaaga ggtgacttcc acgctaccag aaaaacagtg gcaaaacaca ttctgggtcg
    14521 attcggctac tggccaggtt cgtcaaagtc ggcaaatgtt aggcgcaggg gttattcccg
    14581 tagaaatgac gtttcttaaa cccgcaccat gaataaatta cagtcgtatt tcattgccag
    14641 cgtactttac gtaatgacac cccatgcctt tgcgcaagga acggtgacta tttatctgcc
    14701 tggcgaacaa cagacacttt ccgttggccc cgtggagaat gttgcccagt tagtgacaca
    14761 accgcaactg cgcgatcgcc tctggtggcc tggtgccctg ctgaccgact ccgcggtgaa
    14821 agctaaagcg cttaaagact accaacatgt catggcacag cttgcttcct gggaagcaga
    14881 agctgacgac gatgtggcgg caacgattaa atccgtgcgc cagcagttac tcaatctcaa
    14941 tattactggt cgcttaccgg tcaaactgga ccctgatttt gtacgagtgg atgagaacag
    15001 caatcccccg ctggtcggga actatacgct ctataccgta cagcgcccgg tgactattac
    15061 tcttctgggt gcggtttctg gggctgggca attgccctgg caggcaggcc gtagcgttac
    15121 cgattatctg caagatcacc cccgtcttgc aggcgcggac aagaataacg ttatcgtgat
    15181 tacgcctgag ggtgagactg tcgtcgcccc tgttgccctg tggaataaaa gacatgtgga
    15241 accaccgcct ggcagccagc tttggttagg cttttctgcg catgtactgc ccgaaaaata
    15301 tgctgattta aatgaccaga ttgtgtctgt tttaacgcaa cgtgttcctg actgatgaag
    15361 aagaattctt atcttttaag ctgcctggcc attgccgtct ccagtgcctg tcatgctgaa
    15421 gtattaacct acccggatcc gctgggttcg tcgcaatcgg actttggcgg cacaggattg
    15481 ttgcagatgc caaatgcgcg catcgcaccg gaaggtgaat tcagcgtcaa ctaccgggat
    15541 aacgatcaat accgtttcta ctccacctcc gtggcgctgt ttccatggct ggaaggcacc
    15601 attcgttata cggatgtgcg cacacgtaaa tatagccagt gggaagattt cagcggcgat
    15661 cagtcataca aagacaaatc attcgatttt aaacttcgcc tgtgggaaga aggttactgg
    15721 ctaccgcaag tggcgtttgg taaacgtgat attgctggta cgggtctgtt tgacggtgag
    15781 tatctggtgg ccagcaagca agcggggcca tttgatttca ccctcgggat ggcatggggc
    15841 tacgccggta atgcgggcaa tattaccaac ccgttttgcc gggtgagcga taaatattgt
    15901 catcgcgcag agtctcacga tgcgggcgat atcagcttta gcgatatctt tcgtggcccg
    15961 gcttccatct ttggcggcat tgagtatcaa acgccgtgga atcccctgcg tctgaaactc
    16021 gaatacgacg gcaacaatta ccagaatgat ttcgctggca aactgcctca ggcaagccat
    16081 ttcaacgtcg gtgcagttta tcgcgctgcc agctgggcag acctcaacct gagttatgaa
    16141 cgcggtaaca cgttgatgtt tggtttcact ttacggacca atttcaacga tctgcgccct
    16201 gccctgcgcg atacgccaaa accggcatat caacctgcgc ctgaatctga aggattgcag
    16261 tacaccacgg tagcaaacca acttaccgcc ctgaagtata acgcgggctt tgacgcgcca
    16321 gaaattcagc tacgcgataa gacgctgtat atgtctggtc agcaatacaa ataccgtgac
    16381 tctcgtgaag cggtcgatcg tgccaaccgg attctggtga ataacctgcc gcaaggcgtt
    16441 gagaagatta gcgtgacgca aaagcgcgag catatggcga tggtgactac cgaaaccgac
    16501 gtagccagcc tgcgcaaaca gctggcaggt acagcgcctg gtcaatcaga gccactgcaa
    16561 caacaacgtg ttgaagctga agatctttct gcctttggtc ggggctaccg tattcgtgaa
    16621 gatcgcttta gctactcttt caacccaaca ctttcacagt cgctgggcgg cccggaagat
    16681 ttctatatgt tccagctggg gctgatgtcc agcgcccgct actggtttac cgaccacctg
    16741 ctgcttgatg gcggtatttt caccaatatt tacaacaact acgacaagtt taagtcttcg
    16801 ctgttgcccg cggactctac cctgccccgc gtgcgcacgc atatccgtga ttacgttcgc
    16861 aatgacgttt atctcaacaa cttgcaagcg aattactttg ccgacttagg caatggtttc
    16921 tatggccagg tgtatggcgg ttatctggaa acgatgtatg ccggtgtcgg ttccgagctg
    16981 ctttatcgcc cgctagatgc cagctgggcg ctgggtgtgg acgttaacta cgtgaagcag
    17041 cgtgactggg acaacatgat gcgcttcacc gattattcca cgccaactgg tttcgtgacg
    17101 gcttactgga acccgccgac gctcaatggc gtactgatga aacttagcgt tgggcaatat
    17161 ctggcaaaag ataaaggggc aacgatcgac gtcgccaaac gctttgacag cggcgtggcg
    17221 gtaggggtat gggcggcaat cagtaacgta tctaaagatg actacggcga aggcggcttt
    17281 agtaaaggtt tttatatctc gattccgttc gacttgatga ccattggacc taaccgcaac
    17341 cgcgcggtgg tttcgtggac accattgacg cgtgatggtg gacaaatgct gtcacgcaaa
    17401 taccagctct atccaatgac ggcagagcga gaagtaccgg ttggacaata acctgacgta
    17461 attcataaca ttacattgac tgataaatat aaaccaatga aaaagaacat ttttaagttt
    17521 tcggtactga cattggctgt tctgtctttg acagcatgta cccttgttcc tggtcagaat
    17581 ttatcgacca gtaacaagga tgtcattgaa ctacctgaca atcagtatga cctggacaaa
    17641 atggtcaata tctatcccgt cacgccagga ttgattgatc agttgcgcgc caaaccgatt
    17701 atgtcgcagg ctaacccgga gctggagcag caaatcgcga actatgaata tcgtatcggc
    17761 attggtgatg tgttgatggt tacggtctgg gatcacccgg aactaaccac ccccgcaggg
    17821 caataccgta gcgccagtga taccggcaac tgggttaacg ccgatggtgc gattttctac
    17881 ccctatattg gtcgcctgaa agtggccggg aaaacactta cccaggtacg caacgagatt
    17941 acagcgcgtc tggattcagt cattgaaagc ccgcaagtcg atgtcagcgt cgcggcgttc
    18001 cgctcgcaaa aagcgtacgt caccggtgaa gtcagcaaat cgggtcagca gccgattacc
    18061 aatattcctc tgaccatcat ggatgcgatt aacgccgcgg gcggtctgac cgcagatgca
    18121 gactggcgca atgtggtgct cacccagaat ggcgtaaaga ccaaagtgaa cctctatgcg
    18181 ctgatgcagc gtggcgactt gcgccagaac aaactgctgc acccgggaga tattttattt
    18241 attccgcgca acgatgatct gaaagtcttc gtgatgggtg aagtgggtaa acagagcacg
    18301 ctgaaaatgg accgcagcgg tatgaccctg gcggaagcgc tgggtaacgc tgagggtatg
    18361 aatcaggatg tcgctgatgc gacggggatt tttgttattc gtgcaacgca aaacaagcag
    18421 aatggcaaaa tcgccaatat ttatcagctg aatgcgaagg acgcatcggc aatgatactc
    18481 ggaacggaat tccagcttga accttacgac attgtttatg tcactaccgc gcctctggct
    18541 cgttggaacc gtgtgatttc cctgctggta ccgacgattt ccggggtgca tgacctgaca
    18601 gagacttcac ggtggattca aacatggccc aactaaaatt taactcaatc ctggtggttt
    18661 gtaccggcaa catttgccgt tcgcccattg gcgagcgctt gctgcgtaaa cgtctgccgg
    18721 gcgtaaaagt caaatcggca ggtgttcatg gtctggtaaa acaccctgca gatgcgacag
    18781 cggcagatgt tgccgctaat catggcgtat ctcttgaagg acatgccgga cgtaagctca
    18841 ctgctgagat ggcacgaaat tacgatctga ttctggcaat ggagtcggaa catattgctc
    18901 aggttaccgc aatcgctccc gaggtccgcg ggaagacaat gctgtttggg caatggctgg
    18961 aacagaaaga gatcccggat ccctatcgta aaagtcagga tgcatttgaa catgtctacg
    19021 gaatgttgga gcgcgccagt caggaatggg cgaagcgcct cagccggtaa tttgagttta
    19081 taaaatacga tgacaactaa aaatatgaat acgccaccag gcagcactca ggaaaatgag
    19141 atcgatctgc ttcgtctggt cggcgagtta tgggatcacc gtaagtttat tatcagcgtg
    19201 accgcgttat tcacgctgat cgctgtcgct tactcgctgt taagcacacc aatttatcag
    19261 gcagatactc tggtccaggt tgagcaaaaa cagggcaacg ccattctcag cggcctgagt
    19321 gatatgatcc ctaactcatc gcccgagtct gcaccggaga tccaactgct gcaatcgcgc
    19381 atgattctcg gtaaaaccat tgctgaactg aatctgcgcg acatggttga gcagaagtat
    19441 tttccgattg tgggtcgcgg ctgggcgaga ttaaccaaag agaaaccagg tgagctggcg
    19501 atcagctgga tgcatattcc acaactgaat ggtcaggatc agcaactgac actcacggtt
    19561 ggggaaaacg gccactatac gctggaaggt gaagagttca ccgtcaatgg tatggtcggc
    19621 cagcgtctgg aaaaagatgg cgttgcgctg actatcgcgg atattaaggc caaaccagga
    19681 acacagtttg tcctgagcca acgtaccgaa ctggaagcga ttaacgcgtt gcaggaaacc
    19741 tttaccgtta gcgaacgcag taaagaaagc gggatgctgg aacttaccat gactggtgat
    19801 gatccccagt tgattactcg tattctgaac agcatcgcta acaactattt gcaacagaat
    19861 atcgctcgcc aggcggcgca ggattcacaa agccttgaat tcttacagcg ccagttgcca
    19921 gaagtgcgca gcgagctgga ccaggcggaa gaaaaactca acgtttatcg ccagcagcgc
    19981 gattcggttg accttaacct ggaagccaaa gccgttcttg agcagattgt gaacgttgat
    20041 aatcaactca atgagctgac cttccgcgag gcagagatct cccagctgta taagaaagat
    20101 cacccaactt atcgtgcgct gctggaaaaa cgccagacgc tggagcaaga acgcaaacgc
    20161 ctgaataagc gggtatcggc tatgccttcc acccaacagg aagtgttgcg tttaagtcgt
    20221 gacgtagaag cgggccgtgc ggtatatctg caattactta accgccagca ggagttgagt
    20281 atttcgaaat ccagtgccat tggtaacgtg cggattatcg acccggcagt cactcagccg
    20341 cagccagtga aaccgaaaaa agcgttgaat gtggtgcttg gttttattct tggcctgttt
    20401 atttcggtgg gtgccgtgct ggcgcgtgcg atgttgcgtc gtggtgtaga agccccggaa
    20461 caactggaag agcacggcat cagcgtttat gccaccatcc cgatgtccga gtggctggat
    20521 aaacgtaccc gtctgcgtaa gaaaaattta ttttctaatc agcagcgcca tcgtactaaa
    20581 aatatcccct tcctggcggt ggataacccg gcggattctg ctgtggaagc cgtacgtgcg
    20641 ctacgaacca gtctgcattt cgctatgatg gagactgaga ataacattct gatgatcacc
    20701 ggtgcgacgc cagacagtgg taaaacgttt gtcagttcaa ctctggcagc ggtgatcgcc
    20761 cagtccgatc aaaaagtgtt atttattgat gccgacttac gccgtggtta ttcgcataac
    20821 ctgtttaccg tgagtaatga acatggcttg tcggaatatc tggcaggtaa agatgagctc
    20881 aacaaagtga tccagcattt tggcaaagga ggctttgatg tgattactcg cggtcaggtg
    20941 ccacctaacc catctgaact gctgatgcgc gatcggatgc gtcaattact ggaatgggcg
    21001 aacgaccatt acgatctggt gattgttgat acgccgccga tgctggcggt gagcgatgct
    21061 gcggtcgtgg ggcgctctgt cggcaccagc ctgctggttg cgcgttttgg cttgaacacc
    21121 gccaaagagg tgagcttgtc aatgcagcgt ctggaacagg ctggcgttaa tattaaaggc
    21181 gcaatcctca atggtgtgat taaacgcgcc agcaccgctt acagttacgg ctataactat
    21241 tacggttata gttactccga gaaagagtaa aactgaaggt aaagtgaccg gatgcggcgt
    21301 gaacgccttt tccggtctac gcattagaca gttcttcgtt tgtcatcagc ctcagagcat
    21361 tcaggtagct gaatgctctt ttttatacat tacaaactgc acgccggtat gcgtgcttca
    21421 ttcacgattt gcgtaaaacc ggccaacgaa cacatgccct gcgcatttcg ctcttcacat
    21481 cctgccaggg tcagtttcac ctctccgggc ggcgtattta atgacagcgg agttttatca
    21541 cgcatctgct gtaaagtctg gaagaccagc gaaacctgaa tccactggct gttatcgctt
    21601 agccgacgcc agcgttcaaa caccagttca ccacctggcg gcgtgttatc cggctgacct
    21661 ggaagcgtcc agttgagctc cagtgcgccg ccgagatttg ccagattagt atcgtgtccg
    21721 gcgataaaca gcactgaagt gggtaatgtc acaccatacg cctgtttttg cggtggatgg
    21781 ggagtcaacg ctatcatgat caaatccaac aacggggtgg cgcggctgcg ggcaacctct
    21841 ggcgtgcgtt gtagtaaata aaattgcgcg ttatgcaaac ttagcaacgt gttccactgg
    21901 tgtgaatcag tgatccttcc ccaccccggc tccggcattc cctgtgcctg ttgcaggaga
    21961 aatatttccg tcagcattga tgcgaggctt accgcaccgg ttaatgagac attgtcggcg
    22021 ctcaccttga gttccgatgg taatgcctgc gttaatgaac agctttcgtc ctgtttctca
    22081 cggttaaggc acaagtttga ttgcggaaaa ttaagcaccc gttccagttc gcgaaacgcc
    22141 gtttgccgat gcccggtaaa gtcagcaatt gaccctcctg ccctgctgag gatcgcgtca
    22201 gtcacgttcg cgttatccag ttggcaaacg ccagttttta gaggattaaa taacggatcg
    22261 ggactggacg tatctgcctg ggtatgtacg gttattgcac agtcaggtgc cagcccggcg
    22321 gcgaaggctt cgcctgtttt acgggtacgc tcgtcgacat cagcaataat cgcgacctga
    22381 ccaggctgcg ggcagccctt tttcgtcagc aatccgtcgg ctaccagacg ctggcgttgg
    22441 taatgtccga gataagcgat tagctcacca ccgcgcggtg tcagccaacc cagttttacc
    22501 ggccagtttg gccatgcgtc tggggtgaca tcctgcatca gttgcgtggc cttggttggg
    22561 gcacgcacac catgacgact gacaatcacc acactttcca gcttcagctc cggctccggc
    22621 tcactctgag cgaatgcaga ttgcggggtt aacggaatca gaagagataa aaatgggatc
    22681 aagatcgctt tcatcgatat gtttccgctt ttcatatccg acattattga ttgcctgata
    22741 cgacgctaat gcgccgcatc agacttacgt tcgttaactt ttcaatcgcg ggtccagcca
    22801 taccaacaca agggtggaga gcgaacacat caacaaaatg ccgacgaacc aaagtaaata
    22861 ccacattgtt cctgctcctt agtacagctc gttttcgtta cggcggagag tttctgtcgt
    22921 catgcgcccc cacattttgt agtagctcca gagagtgtag agcaacacaa tgggcaaaaa
    22981 tatcagcacg attaccaaca taatactcag cgtcagctga ctggaagtgc tgtcccacaa
    23041 cgtcaggctg gagattggac tcacgcttga cggcatgaca aaggggaaca gcgtgatgcc
    23101 tgccgtgaaa atcacgccaa attgcatcaa tgacgccatc aaaaatcccc aacccgggcg
    23161 gccacgataa atcgccatca ccgtcagcaa tgggcagaag aaccccagca gcgggaagat
    23221 ccacaaaacg ggcgactcga caaaattatt catccaggca ccaggtagca ctgccaccag
    23281 tttcattaac ggattggaag gaccgttagc atcctgggcg agcagtacaa agccatcaat
    23341 accgacccac agccagtaac ccgccagcaa aaagcacagc atcaccaaca gtgcagcgcg
    23401 tttagtcgac agctgtgaac gcagatgaat cacaccaaca gttttcagtt gtaaccagac
    23461 gccaccttgc aaaatcacca tcccaaggct gagcaatccg cacaataaag ggaatggcgt
    23521 cagcagttgc cagaagctgc cgagatactc cacgcgtaat tgcggagtga aggcaaacgg
    23581 cacgccgagc aacaagttgc cgaaggcgat accgaagact accggcggca ccagactgcc
    23641 gatgaccaga cctgcatccc acattttacg ccagcgtgca tcggcgattt ttccgcgata
    23701 atcaaaggcc agcgggcgga agaacagcga gcacagcacc aggatcatcg ccacataaaa
    23761 gccggaaaac gccgctgcat acactctggg ccaggcggca aataatgccc cacctgcgag
    23821 gatcaaccag acctgattgc cttcccagtg cgcaccaacg ctattaatca ctatccggcg
    23881 ttcatcatca tcacgcgcca ccagcggcag caaacagcca atccccatgt caaatccgtc
    23941 ggagatcata aagaccacca ggatcacgcc aatcagcagc caccagatga agcgcaatgt
    24001 ttcataatca aacatgattt tctcctttac ccctgttgct gcgtcggttg ttcactctgc
    24061 atcgcgctcg gccccagacg ggcatatttc tgcatcaggt agacttcggc gattaagaac
    24121 agggtgtaaa gccctacgat catgatcagt gagaaagcca gttgtcctgt ggttaaagcg
    24181 gagtgcgcgg agtatgtcgg taagatgtcc tgtatcgccc acggctgacg accaaactcg
    24241 gtcataaacc acccggcttc aatcgcaatc cacggcaacg gcaaactcca gagcgccatt
    24301 ttcagcaccc agcgatgctg gtcgattttg ccacgcagcg tctggacaag cgcaatcagc
    24361 atcaccagta gcagcaggga accacagccc accatgatgc ggaaactcca gaataccggc
    24421 gcaacctgag gtatcgcgcc acgcatcgcc gcctggtact gtgcggctgt gacatgattc
    24481 atatccggcg catagcggga gagcaacatg ccgtagccca ggtcgccttc cagctcccgg
    24541 aatgcctgca acacatgcgg ctcacgattg ccttgcgata tttcctgcat taacagccag
    24601 gccatacgtc cgcgttgcaa gcgtgggtag gtttcagcca tcaaattctt cagacccggc
    24661 acgggtttat ctaatgagtg agtggcgagg atccctaaca gcacgggaat tttgatggca
    24721 aaggcgttac gctcttgatc ctgttccggc caggcaacca catggaacgg tgcaggagca
    24781 ggttccgttt gccactcccc ttccatcgcc gccagtttta ccggttgtac ttgcgcgact
    24841 tcatacgcag aactgtctcc gagttgcagg gtaccgataa tcgccagagt accgaagacg
    24901 gaaccgatgg caaacgagcg taatgcgaca tcgcgctccc gtccgcgcag taaataccag
    24961 gcgctgatcg ccataataaa catggccccg gtcacgtagc ccgccattac ggtgtgcaca
    25021 aatttcacct ggctgaccgg gttaaagacc agctcgctga agctggtcat ctccatacgc
    25081 agggtgtcga tatcaaaatg cgcaccggtc gggtattgca tccaaccgtt ggcattcaat
    25141 atccacaatg cagagagatt tgaaccgaac gccaccagcc acgtcaccag caggtgctgg
    25201 tatttattca gccgctgcca gccgaagaag aaaagcccga caaaggtgga ttcgaggaag
    25261 aaggccatta atgcttccat cgccagcggt gcgccaaaaa tatcgcccac atagttggaa
    25321 tagaatgacc agttagtacc aaactgaaac tccatggtca ggccggtagc cacgccaaga
    25381 gcaaagttga taccgaagag cttaccccag aagcgcgtca tatcgcggta gattgttttg
    25441 ccggtgacca cgtaaatggt ttccatgata gccagcaaaa aaatcagccc cagggtaagg
    25501 ggtacaaata aaaagtgata cagcgcggtc agagcaaact gccagcgcga taaatcaatg
    25561 acatcccaca tgcgcactcc tgtaggcgaa gagagaaaaa taacccgtta atggtagatc
    25621 tccctcttta tcctgaaact ctacgaccag aggtattcgc cgattgcgat attcataagc
    25681 ctgaaagcga tacttaccgt ctttttacgt cggtgcagct tccgccagcc actgacatac
    25741 ctcactaagc cgctgcgcag agtcgaccaa atcttctggc gctgccagca ccacttccgg
    25801 tattgggcag atttcataac tttccagtaa cgggcctttt aaggtgtccg tacagcgtaa
    25861 atgccagaca tggcgtaacc ccgtggcgtt gatatagctc tcgccatagc caatggtacg
    25921 aatctgaata tttcccggcc cacagagacg tgagagaaaa agccgatcag cctcgctgat
    25981 gggtaattgc gtcagattaa tgctgtgcgg ctgcgcgtcg gggttacgta catgcgccag
    26041 taactcatgc gccaacggta ggccattcat caggccatca atgggcggcg gtaacagcga
    26101 atctgtcggc aagagatttt gcgttgccgc ctgccacaac gccagcggcg cgcagccagc
    26161 ctccagtttg tcctccagca acttttcgcc gcgacgtctg cgcacccgcc ataatccgca
    26221 gaagatcgcc tcctgtattt cactttcgct gtcgtcagcc tgctgaatgc gcactgacac
    26281 ttcaccttcc ccgagaagcg tattgataaa gtgacgatcg tcggcgttga gatttaacag
    26341 atcgactgcc agcacctcgc ccaacggcgg ctggcgttga cttagttgat gttgtaactc
    26401 gtttaacagc gcaatcacct gcgggctgtg agcacattgc tccagggccg ccatactcgg
    26461 ttcatcattc acctgacagg tgatcggcag tggattcatg ctgaaactgt catcgttcgg
    26521 ttgcgttctt ggccccagca gatggaaaaa agtttcgctc atgaggcacg ctcctgctgt
    26581 ggttcgacaa gcccacgcat caggtttatc agttccgccc acgggtgaat accattcagc
    26641 acgccgcgat agtttccgcc ggtaaacact aatgtggcgg gaaagcgaaa gacgccaaag
    26701 cgatctccga tggcttcgct ctgttcaagg tcagcaatcg ccacctgcca tgtgtagtcg
    26761 ggaaactcgc gcagtaattc gccaatcatt accggattat cgctgacctc tggcgtgcgt
    26821 ttcgggtcac tgcttaataa caccacgccg tctggcgctt gcgtaagcca gtcgtcaaga
    26881 cggaattcac taaccggcgt ccagccgcgc gccagcattc gttgccacaa cgcatcaaat
    26941 ggcgtgtcgt tgctcatccc tgctcctcca gtgtcatcct gtactggcgc aagcgaacgc
    27001 cttcgtaatt ttccatcgac agacagtcat aattgagaca gcgcgattca ttagccggtt
    27061 gcggcacaat tccccatgca gccagctgcg ccagcgccgc ctgttccgct gcgggcagtt
    27121 gctcccgtgc cagttcgctc aggctgccgc cgtagtcgtc gagcattgcg ggttgcagac
    27181 cgacgagggc aatatgtgct ggcagatgtc cgcggatatc tgccagcgcc agcacttcgg
    27241 agaaactgtt ctggtgcagg ctcattttct tcgcgctgag ataagccgga atacgttctc
    27301 cggcataggt tcgcagcgtt cctggttcca gcccgtagtc aatggcatcg agaatcaaca
    27361 gatggctggc gctttcgaca taccccagca agttcagtcc ctgagtaccg ccatcgacaa
    27421 tctccacaga ctcgggccag tggtaatggg catacaaccg ttccgccacc cgcacgccga
    27481 aaccttcatc ggcccacagc aggttgccca gccccattac caccacgcgt tgctcgctca
    27541 tgaacgctcc ttgttactta ttttgccaaa tttgtggcta cggtagccgt tgaccatggt
    27601 ggagatcacc gtgtcgtcgg acatgatgtc ttcacgcagc gccatataga catgaccgat
    27661 cacaaacgcg ccaatcagcc acatccccag ccgatgccag ctgtgaatgt ccattgagtt
    27721 gcctcccgtc cagtagaaaa attccaccac ataacggaac ggcgcaaaaa tagcgtactg
    27781 gctgtgttcg ctgtacagcg caaagccggt gatgatcata aagaccgaca tcaggaaata
    27841 gccaaacatc gccgcctggg cgatggggtt atggcctata tcggcactcg gacgttttgc
    27901 cagaaacaga taccagcgga tttcatacca cacgccctgc caccagcttt tacgccatac
    27961 tggcacgata aacagctcgc gggagtatcg attgccaaca aaagcccagt agatccgcat
    28021 cagcaagacc acggtaaaaa ccatcccggc gctgaagtga attaacctga tgtagcccat
    28081 atagaacaga tacgtcgcct cgccgctgac ggaaggtagc ggtttgccga taaagtaacc
    28141 ggtgaccatc aacaccgcca tgcataacac cgtcagccag tgccagatac gcactggcgc
    28201 ttcaaagaca tagtggctga caacgttgtc gcttttctgt tgcatgatga ttctccttcg
    28261 ctgttaacgc acctgcacgg agatcagctc gctaccgtcg tcgcccagca cgtgtgttga
    28321 acaggcgagg cacggatcaa agctgtgcag agtacgcagg atctccagcg gttgctcggg
    28381 gatcgccatt ttggtgttca tcagcgccgc ttcataagcg ccaatctgcc ctttgggatc
    28441 gcgcgggctg gcgttccagg tggtcggcac cacgcactgg tagagatcaa tcttgccatc
    28501 gcgaatggcg gcccagtggc ctaacgcccc gcgcggcgct tcggtaaaac cgacaccacg
    28561 gcactctgtc ggccaggttg tgggttccca tttttccgtg gaggcggtgg caagattgcc
    28621 gtttttcagg ttagtcatca gcttgttgaa gaaatactgc aacttacctg cggcccactg
    28681 tgcttcgtgc gcgcggcaca aaatgcggcc caacgttgac tggataccgg aaagcggcag
    28741 gttcagcgcc gacatcatgc gatcgaccga ctcaacggtc gcagcatcgc ctttgtgata
    28801 agcgattaac gtgcgtgcca gcggccccac ttccatcgcg ttaccgcgcc agcgcggcgc
    28861 tttgatccac gagtagcgtt cctgttcatt cagctgctga atgttggtat cgctgccttt
    28921 gacatcgccg gggttgtacc acgggtcggt gatgccatcg aacggatgac gcccgacctg
    28981 atcgttggga taacgatacc aggcgtgatc gacaaactcc tgcacctgct gcggatcaac
    29041 caaatccact ggcagcacat tgttgaagtc gccgttaatc accgcgccgc caggcatcag
    29101 cagacttttc tcaccaaagt cgttggcaat atccgggaat gcgccgtagc tgaggacaca
    29161 tttatcagaa agacccgtgc cgatttcgct ccacggtttg ttgaactgac cgatggctaa
    29221 ggcgtcgggg atcatcacgt tgttaatgaa gtctgccgtg cgggtgataa tcgactgcac
    29281 caggttcagg cgttccatat tgaccgcccc gaccgcgccg ctttcgtcaa tgttaatggc
    29341 gcaaggcatc ccgccgacaa tccagtttgg atgcgggttt ttaccgccaa agaccgcgtg
    29401 gattttgaca atttcacgct ggaaatcgag agcttcgaga tagtgggcaa agcccatcag
    29461 gttggcttct ggcggcaact tgtactgcgg gtgcccccag tagccattgc ggaagatccc
    29521 caactgcccg ccttcaacga atttcttcag gcggttttgt acgtcgaaga aatagccagg
    29581 ggatgatttc ggccaagagg agagactttg cgccagttcg gaggttttcc gcgggtcggc
    29641 tttcagcgca tccaacacat cgatccagtc catcccggca agctgataga agtgcaccag
    29701 atgatcgtgg caccagagcg ttgccagcat aatgttgcgg atgatattgg cgttgtccgg
    29761 cactttaata ccgatggcat cttcgatggc gtaaaccgaa gccagagcgt gtacgccagt
    29821 acagacgccg cagatacgtt caacgaacgc ccacgcatcg cgcgggtcgc gcccttgcag
    29881 gatgatctcc agcccgcgaa acatggtgcc gcaggagacg gcattggtga ttacattctg
    29941 atcgttaata ttcacttcgc agcgcatgtg gccttcaatg cgcgtaatcg ggtcgaccac
    30001 caggcggcgt ccggcattat tgatggtgta tccctgagtt tcgtactgag tgctcatgcc
    30061 tgtttatcct cattgcctgg ctgatgttcg gtttctgtag gttgctgatt atgacgtctg
    30121 cgctggtcaa cggcgctggc gactgcgtgc acaccaacag ccgctgccac cacaccaagc
    30181 gcggttaaac cgacggtatc ggcggtggaa tgagtaccca tttgtggaat atcgaccacg
    30241 cggctgtaga acgaaccgcg atcccagaaa ccattttccg cacagcccag gcagccgtga
    30301 ccagactgga ttgggaaaga aacgccatca ttccagcgtg tggaggaaca ggcgttatag
    30361 gtggtaggcc ctttgcagcc cattttgtac aggcaataac ctttgcgggc agcgtcatca
    30421 tcccaactct ggacgaactc tccggcgtcg aagtgagcgc ggcgatagca tttatcgtgg
    30481 atacgctgac cataaaacat cagtggacga cccattctgt cgacatctgg caagcgatca
    30541 aaggtcacca tgtaagtaat gatggcgctc atcacatccg ggatcggcgg gcagccaggt
    30601 actttgataa tgggtttgtc ggtgatgact ttgtcgatag acgttgcctg tgtcggatta
    30661 ggtcgcgcgg cctgcacgca gccccaggac gcacaggttc cccaggcgat aatcgcgctg
    30721 gctccggcag cggcacgttt gagtttctca ataaacggtc gaccgctgct gatacagaac
    30781 atcccctgct cgcccagcgg cggattacct tctactgcga ggatatattt gccattgtat
    30841 tgcgtgatga tgtcttcaaa gacttcttcc gcctgggttc cggcggcagc catcaaagta
    30901 tcgtcgtaat cgagggaaat cagggaaagg atgacgtcct ttgccagcgg gtgagcggag
    30961 cggataaaag attcggtaca gcaggtgcat tccagaccgt ggatccatac caccggaatg
    31021 cgcggtttgt tctccagcgc ccaggcaatc tttggtgcca ttcccgcgcc taatcccagc
    31081 gacgtggcag ccagactaca atatttgaga aagctgcgcc gggtaacgcc ctgacgccgc
    31141 atggcctggt aaaatgtttc ctcgttattc atatcgcacg tctcttctcc ttgcgacacc
    31201 ggcaggacac cgggcgctta tggtcggcaa gcgacgtccc tgtagcaagg caaaccatcg
    31261 cttcacgtga gatgctgaaa acgaaagctc atccttctgc actggcgcac gtcgccagaa
    31321 agtattgtta ataaagcgta gtgaaacttt tgcacaaaac aatacaaact gtgtggattt
    31381 atcttttagc gataaaaatg gacctatttt tctttttgcc gggcggtggg atgtttagcc
    31441 agttgctaaa cgagtaaaag agaaggaatt tatggtgatg aaatgatcaa tctgctttta
    31501 tttcacgcta tcaatgcact tttggctgtt tttcaggcaa acaaacagat taggggttta
    31561 cacgccgcat cgggatgttt atagtgcgcg tcattccgga agtgtggccg agcggttgaa
    31621 ggcaccggtc ttgaaaaccg gcgacccgaa agggttccag agttcgaatc tctgcgcttc
    31681 cgccaaataa tcaaggggtt accaaatggt agcccctttg cttttttggc tcttggtata
    31741 aacttggtat accaattggt atatttttat tcggatgaga ttatcggtca tgagcggaac
    31801 ctcaggttcc tcgtctgatg ccgctcttgc aacacgttac gctgccgagt atttttgtaa
    31861 aacatggact gctccaggcc tgaaccaggc agaaggatac aaagccatct cagatctgtc
    31921 gcatcactat tttcgtgcag aaggctctag ccctccccaa tcttttttgt gacaccctgt
    31981 ttcactgaac atgaagacac tcaccttgtc cctgatattc attagcatca tcctgattat
    32041 ttaacttctc tatttaggga agcccgtctg atacaaagct tctattcctc gttaaatatt
    32101 ctccattctg gcgccaagcc ctatctatag
//
LOCUS       DBFIFI010000034        32096 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.034, whole genome shotgun sequence.
ACCESSION   DBFIFI010000034 DBFIFI010000000
VERSION     DBFIFI010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32096
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.034"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(62..1822)
                     /gene="ycaO"
                     /locus_tag="N7B39_001511"
     CDS             complement(62..1822)
                     /gene="ycaO"
                     /locus_tag="N7B39_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HGU2421517.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDERLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            1952..2644
                     /locus_tag="N7B39_001512"
     CDS             1952..2644
                     /locus_tag="N7B39_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HGU2421518.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKSGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            2838..3926
                     /gene="serC"
                     /locus_tag="N7B39_001513"
     CDS             2838..3926
                     /gene="serC"
                     /locus_tag="N7B39_001513"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HGU2421519.1"
                     /translation="MAQIFNFSSGPAMLPVEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NVACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            3997..5280
                     /gene="aroA"
                     /locus_tag="N7B39_001514"
     CDS             3997..5280
                     /gene="aroA"
                     /locus_tag="N7B39_001514"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HGU2421520.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            5449..6213
                     /gene="ycaL"
                     /locus_tag="N7B39_001515"
     CDS             5449..6213
                     /gene="ycaL"
                     /locus_tag="N7B39_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HGU2421521.1"
                     /translation="MKNTKLLLAIVTSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            6386..7069
                     /gene="cmk"
                     /locus_tag="N7B39_001516"
     CDS             6386..7069
                     /gene="cmk"
                     /locus_tag="N7B39_001516"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HGU2421522.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            7180..8853
                     /gene="rpsA"
                     /locus_tag="N7B39_001517"
     CDS             7180..8853
                     /gene="rpsA"
                     /locus_tag="N7B39_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HGU2421523.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            9013..9297
                     /gene="ihfB"
                     /locus_tag="N7B39_001518"
     CDS             9013..9297
                     /gene="ihfB"
                     /locus_tag="N7B39_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HGU2421524.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            9504..11768
                     /locus_tag="N7B39_001519"
     CDS             9504..11768
                     /locus_tag="N7B39_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HGU2421525.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILLAGETLTGATQDAIVEITATDGMTTHYGQITYLQ
                     GQRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRSGQIFLPGRWIHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVVILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILDQGLWFLADRSLALLFLGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSAHWPLQGRNDKGNNHSCVVK
                     VDDGTNSILLTGDIEAPAEQKMLSRYWQQVQATLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQQQGYTWLDTPHQGQVTVDFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            11805..13553
                     /gene="msbA"
                     /locus_tag="N7B39_001520"
     CDS             11805..13553
                     /gene="msbA"
                     /locus_tag="N7B39_001520"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HGU2421526.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            13550..14536
                     /gene="lpxK"
                     /locus_tag="N7B39_001521"
     CDS             13550..14536
                     /gene="lpxK"
                     /locus_tag="N7B39_001521"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HGU2421527.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     STGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLAQLTSLASGN"
     gene            14573..15805
                     /gene="ycaQ"
                     /locus_tag="N7B39_001522"
     CDS             14573..15805
                     /gene="ycaQ"
                     /locus_tag="N7B39_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HGU2421528.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEYPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     QGIFREQWLADYYRLKRPALAAWREARAEQRQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTTLQKGLRQAITDFANWQQATRVTLGHC
                     PQGLFTDCRTGWEIDPVA"
     gene            15857..16039
                     /gene="ycaR"
                     /locus_tag="N7B39_001523"
     CDS             15857..16039
                     /gene="ycaR"
                     /locus_tag="N7B39_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HGU2421529.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            16036..16782
                     /gene="kdsB"
                     /locus_tag="N7B39_001524"
     CDS             16036..16782
                     /gene="kdsB"
                     /locus_tag="N7B39_001524"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HGU2421530.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAKGLETVGDNFLRHLGIYGYRAGFIRRYVTWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAHEVPGTGVDTPEDLERVRAEMR"
     gene            16936..17829
                     /locus_tag="N7B39_001525"
     CDS             16936..17829
                     /locus_tag="N7B39_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HGU2421531.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(17806..18585)
                     /gene="elyC"
                     /locus_tag="N7B39_001526"
     CDS             complement(17806..18585)
                     /gene="elyC"
                     /locus_tag="N7B39_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HGU2421532.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGSSGEPRQE"
     gene            18721..19506
                     /gene="cmoM"
                     /locus_tag="N7B39_001527"
     CDS             18721..19506
                     /gene="cmoM"
                     /locus_tag="N7B39_001527"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HGU2421533.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPTQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            19503..20825
                     /gene="mukF"
                     /locus_tag="N7B39_001528"
     CDS             19503..20825
                     /gene="mukF"
                     /locus_tag="N7B39_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HGU2421534.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALSYANADRLLDMRDEEMALR
                     DEEVTGELPPDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            20806..21510
                     /gene="mukE"
                     /locus_tag="N7B39_001529"
     CDS             20806..21510
                     /gene="mukE"
                     /locus_tag="N7B39_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HGU2421535.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDIDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            21510..25970
                     /gene="mukB"
                     /locus_tag="N7B39_001530"
     CDS             21510..25970
                     /gene="mukB"
                     /locus_tag="N7B39_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HGU2421536.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            26231..28078
                     /gene="ldtD"
                     /locus_tag="N7B39_001531"
     CDS             26231..28078
                     /gene="ldtD"
                     /locus_tag="N7B39_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HGU2421537.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGILPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQQALDKGQLPTFVAGLAPQHPQYA
                     VMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            28259..28807
                     /locus_tag="N7B39_001532"
     CDS             28259..28807
                     /locus_tag="N7B39_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HGU2421538.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            28834..29481
                     /gene="gloC"
                     /locus_tag="N7B39_001533"
     CDS             28834..29481
                     /gene="gloC"
                     /locus_tag="N7B39_001533"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HGU2421539.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDA
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(29532..30722)
                     /gene="aspC"
                     /locus_tag="N7B39_001534"
     CDS             complement(29532..30722)
                     /gene="aspC"
                     /locus_tag="N7B39_001534"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HGU2421540.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRIAADFLAKNTSVKRVWVSNPSWPNHKSVFNSADLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(30907..31995)
                     /gene="ompF"
                     /locus_tag="N7B39_001535"
     CDS             complement(30907..31995)
                     /gene="ompF"
                     /locus_tag="N7B39_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HGU2421541.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQLLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNISGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         7802 a         7822 c         8634 g         7838 t
ORIGIN      
        1 aaacgccccg taatataact cagacgaatc attaagccta cattacgtag gctatttgat
       61 tttattttgc ccagaatgct gctttggcgc gctgcagctt ttcgtaggcc ttcaacagcg
      121 actgatgtgc agcaaacgcg tgcagatcgc tatctactgg ttgcaagcca taaaacgccg
      181 cttcgccgct cattgccgca ctggcggctt ccacggcatc tgcgccgtac atgcgaacaa
      241 acgcattcag atattgcagc ggctggcgat cttcttcctg cgccagtaat aacaacgttt
      301 gcaggcagcg ataatagttg gcgcgttccg ggctgaatac cgatgagtta aactccatcg
      361 tccattcggt ccagaccaga gcctgttcca gatcgccacc agccagcgcc agcatggctt
      421 ttaattcacc aatacgcagg gtgtaccaac cgttatccga cccggtcgcc agacccaaca
      481 gctcacgcac gcgggtaaag tcatcaaaac cttcttcatc cagttgctcg atgaggttca
      541 ggtaatcttc tttttcccac tcgctgcctg gtagcgaaag aatcgtttca cgtaaatggc
      601 tgcccatact gttattcgcg agccacagat cttcagccgg ataaatatcg gacatgccag
      661 gcacgataat acggcaagca taaacgccca gatgctcgta atcggcaata taaacttctt
      721 tatcttcttt gttgaagata gccatcagcg tagcaaactc ttcttccgtg gtgccggaga
      781 aattccagtc cacaaacgga taatcggcat cctgcttgaa caggtcccag gagattaaac
      841 cgctggaatc gataaagtgc gtttcgaggt tggtatgttc agcgacttct tcatcatcga
      901 aggttggcgg agtaaacaca tccaaatctt tcaggccacg accttgcagc agctcggtca
      961 cggtacgttc cagtgctacg ccaaaatcag gatgcgcacc gaaagaggca aagcaggtgc
     1021 cgttagcagg attaaacagt accacgcaaa tcaccggata ctggccgcca agggaaccat
     1081 catatgcgaa gatcgggaaa ccttctgctt ccagtgtttc gatcgcttca accaccgctg
     1141 ggtaacgcgc cagcacgtct gccgggattt ccggcaagct gatgctttca gcaataatgc
     1201 ggtttttcac gtagcgttcg aaaacttcgg acaacccctg aacgcgtgct tcgttacggg
     1261 tattgccagc agacataccg ttagaaacgt acaggttacc aatgatattc atcggaatat
     1321 aaacggtctg attatcagac tgacgtgtaa acggcagacc gcaaatacca cgatcttcgt
     1381 taccggattg taggtcaatc agcatactgc cggtcaattc attctccgga tcgtaaaatg
     1441 cgcgcagacg ttcatcgagc agcccttctg gcacatcgtc attttcggtc agtgggaacc
     1501 atttttcgtt gggataatgc acaaatggac cgttggcgat ggtttcgccc agccagaaat
     1561 cagcaaaaaa gtagttggtt gagagacgct cgaaatattc accgagtgca gaagccagcg
     1621 ccgctttctt ggttgcgcct ttaccgttgg taaaacacag tgcgcactct ttgtcgcgaa
     1681 tatgtacaga ccagacgtta ggcacgggat tcagccagga ggcctcttca atctgaaagc
     1741 cgaggtctga aagtttttgc tggaagcgag cgatggaatc ttccagtgcg gcatctttgc
     1801 cggggataaa tgtttgcgtc atgaaaatca ctttagtcgt acggaaagcg cgcaataata
     1861 cgggttttat ctcaaaggcg ctatcaccgc cgccatgccg atgaactgtt gactatgctt
     1921 ttagcggata aaccacgtta agagcataaa aatgaaggcg ttcgatctcc accgtatggc
     1981 atttgataaa gtgccttttg atttccttgg cgaagttgca ctacgtagtc tttatacctt
     2041 tgtactggtc tttttgttcc tcaaaatgac cggaagacgc ggtgtgcggc agatgtcgct
     2101 gtttgaagtt ttaatcattc tgacgctggg atcagcggcg ggagatgtgg cgttttatga
     2161 tgatgtgccg atggtcccgg tacttatcgt ctttattact ctggcgttgt tataccgcct
     2221 ggtaatgtgg ttgatggcgc acagtgaaaa actggaagat cttctggaag gcaagccagt
     2281 tgtcattatt gaagatggcg agctggcctg gtcgaaactc aataactcca acatgacgga
     2341 atttgagttc tttatggagc tacgattgcg tggcgtggag cagctggggc aggtacgtct
     2401 ggcgattctc gaaaccaacg ggcaaatcag tgtctatttc tttgaagatg acaaggtgaa
     2461 atcgggttta cttattttac ccagtgattg tacgcagcgt tacaaagtgg tgccggagtc
     2521 ggcggactat gcctgtattc gttgtagtga aatcattcat atgaaagcgg gggaaaaaca
     2581 attatgtccg cgctgtgcaa atccagaatg gacgaaggca agtcgggcaa aacgggtgac
     2641 ctgacagtaa aaacatcggc tttttgctaa taatccgaga gattcttttg tgtgatgcaa
     2701 gccacatttt tgccctcaac ggttttactc attgcgatgt gtgtcactga atgataaaac
     2761 cgatagccac aggaataatg tattacctgt ggtcgcaatc gattgaccgc gggttaatag
     2821 caacgtggtg aggggaaatg gctcaaatct tcaattttag ttctggtccg gcaatgctac
     2881 cggtagaggt gcttaaacag gctcaacagg aactgcgcga ctggaacggt cttggtacgt
     2941 cggtgatgga agtgagtcac cgtggcaaag agttcattca ggttgcagag gaagccgaga
     3001 aggattttcg cgatcttctt aatgtcccct ccaactacaa ggtattattc tgccatggcg
     3061 gtggtcgcgg tcagtttgct gcggtaccgc tgaatattct cggtgataaa accaccgcag
     3121 attatgttga tgccggttac tgggcggcaa gtgccattaa agaagcgaaa aaatactgca
     3181 cgcctaatgt ctttgacgcc aaagtgactg ttgatggtct gcgcgcggtt aagccaatgc
     3241 gtgaatggca actctctgat aatgctgctt atatgcatta ctgcccgaat gaaaccatcg
     3301 acggtatcgc catcgacgaa acgccagact tcggcaaaga tgtggtggtc gccgccgact
     3361 tctcttcaac cattctttcc cgtccgattg acgtcagccg ttatggtgta atttacgctg
     3421 gcgcgcagaa aaatatcggc ccggcaggcc tgacaatcgt catcgtccgt gaagatttgc
     3481 tgggcaaagc gaatgtcgcg tgtccgtcga ttctggatta ttccatcctc aacgataacg
     3541 gctccatgtt taacacgccg ccgacatttg cctggtatct atctggtctg gtctttaaat
     3601 ggctgaaagc gaacggcggt gtagctgaaa tggataaaat caatcagcaa aaagcagaac
     3661 tgctatatgg ggtgattgat aacagcgatt tctaccgcaa tgacgtggcg aaagctaacc
     3721 gttcgcggat gaacgtgccg ttccagttgg cggacagtgc gcttgacaaa ttgttccttg
     3781 aagagtcttt tgctgcaggc cttcatgcac tgaaaggtca ccgtgtggtc ggcggaatgc
     3841 gcgcgtctat ttataatgcc atgccgctgg aaggcgttaa agcgctgaca gacttcatgg
     3901 ttgagttcga acgccgtcac ggttaatgcc gaaattttgc ttaatcccca cagccagcct
     3961 gtggggtttt tatttctgtt gtagagagtt gagttcatgg aatccctgac gttacaaccc
     4021 atcgctcgtg tcgatggcac tattaatctg cccggttcca agagcgtttc taaccgcgct
     4081 ttattgctgg cggcattagc acacggcaaa acagtattaa ccaatctgct ggatagcgat
     4141 gacgtgcgcc atatgctgaa tgcattaaca gcgttagggg taagctatac gctttcagcc
     4201 gatcgtacac gttgcgaaat tatcggtaac ggcggtccat tacacgcaga aggtgccctg
     4261 gagttgttcc tcggtaacgc cggaacggca atgcgtccgc tggcggcagc tctttgtctg
     4321 ggtagcaatg atattgtgct gaccggtgag ccgcgtatga aagagcgccc gattggtcat
     4381 ctggtggatg cgctgcgcct gggcggggcg aagatcactt acctggaaca agaaaattat
     4441 ccgccgttgc gtttacaggg cggctttact ggcggcaacg ttgacgttga tggctccgtt
     4501 tccagccaat tcctcaccgc actgttaatg actgcgcctc ttgcgccgga agatacggtg
     4561 attcgtatta aaggcgatct ggtttctaaa ccttatatcg acatcacact caatctgatg
     4621 aagacgtttg gtgttgaaat tgaaaatcag cactatcaac aatttgtcgt aaaaggcggg
     4681 cagtcttatc agtctccggg tacttatttg gtcgaaggcg atgcatcttc ggcttcttac
     4741 tttctggcag cagcagcaat caaaggcggc actgtaaaag tgaccggtat tggacgtaac
     4801 agtatgcagg gtgatattcg ctttgctgat gtgctggaaa aaatgggcgc gaccatttgc
     4861 tggggcgatg attatatttc ctgcacgcgt ggtgaactga acgctattga tatggatatg
     4921 aaccatattc ctgatgcggc gatgaccatt gctacggcgg cgttatttgc aaaaggcacc
     4981 accacgctgc gcaatatcta taactggcgt gttaaagaaa ccgatcgcct gtttgcgatg
     5041 gcaacagaac tgcgtaaagt cggtgcggaa gtagaagagg ggcacgatta cattcgtatc
     5101 actccaccgg aaaaactgaa ttttgccgag atcgcgacat acaatgatca ccggatggcg
     5161 atgtgtttct cgctggtggc gttgtcagat acaccagtga cgattcttga tcccaaatgt
     5221 acggccaaaa catttccgga ttatttcgag cagctggcgc ggattagcca ggcagcctga
     5281 atgaataacg tccaataaat agccaaaact ttctttatca aaacgtcggc acattgccgg
     5341 cgtttttttt cggaccttgt gagtcatttt gattaatggt agcgtcgctt gtcaatgtaa
     5401 gttgttgata cataatgttt atatatgatt aatcaacgga tgattcacat gaagaatact
     5461 aaattactgc tggcgattgt gacctctgca gcattactga cggggtgtca aaatacccac
     5521 ggtattgata ccaatatggc tatcagctcc ggtttaaatg cctataaagc agcaacgtta
     5581 agcgatgccg atgcaaaagc gattgccaat cagggctgtg ccgaaatgga cagcggcaat
     5641 caagtcgcaa gtaaatccag caagtacggt aaacgtctgg caaaaatcgc caaagcattg
     5701 ggtaacaata ttaatggcac gccggtcaac tataaggttt atatgaccag cgacgtcaac
     5761 gcatgggcga tggcgaacgg ctgtgttcgt gtttacagtg gcctgatgga catgatgaac
     5821 gataacgaaa ttgaaggcgt tctgggccat gaactgggcc acgtcgcgtt gggtcactcg
     5881 ctggctgaaa tgaaagcttc ttatgcgatc gttgccgcac gcgatgccat ttcagctacc
     5941 agcggtgtgg cttcccagct ttcccgctca caattgggtg atatcgcaga aggcgctatc
     6001 aatgcgaaat actcccgcga taaagagtcc gaagcagatg atttctcctt tgatctgttg
     6061 aagaaacgtg gcatcagcac ccaggggctg gttggcagct ttgaaaaact ggctagcctg
     6121 gatggcggtc gcacccagtc catgtttgac tctcacccac catcaacaga gcgtgcgcaa
     6181 cacatccgtg atcgtatcgc ctctggtaag taaatcattg tcatctttcg ggctggtctt
     6241 ctgccagccc gctataattg cgcaataaat ccccatctga atacagacaa aactggtttt
     6301 tgcacacaac gttaacgatt tgtggcgtcg gcgcgtataa tgcgcgcggt tatgttaacg
     6361 gtacgcctgt tttaaggaga taaagatgac ggcaattgcc ccggttatta ccattgatgg
     6421 cccaagcggt gcagggaaag gcaccttgtg taaggctatg gcggaagcgt tgcaatggca
     6481 tctgctggat tcgggtgcaa tttatcgcgt actggcacta gcggcattac atcaccatgt
     6541 tgatgttgca tcggaagatg cgctggtgcc gctggcatcc catctggatg tacgttttgt
     6601 gtcaaccaat ggcaatctgg aagtgatcct cgaaggggaa gatgtcagcg gcgaaattcg
     6661 tactcaggaa gtggcgaatg cagcttcaca agtcgcggca ttcccacgcg ttcgtgaagc
     6721 attattgcgt cgccaacgcg cgtttcgcga attaccaggc ctgattgccg atggccgcga
     6781 catgggaacg gtggtattcc ctgatgcgcc agtgaaaatt ttccttgacg cctcctcgga
     6841 agaacgtgcg catcgccgca tgctacagtt gcaggagaag ggctttagtg ttaactttga
     6901 gcgccttttg gccgagatca aagaacgcga cgaccgcgat cgtaaccgag cggtagcgcc
     6961 actggttccg gcagccgatg ctttagtgtt ggattccacc accttaagca ttgagcaagt
     7021 gattgaaaaa gcgctacaat acgcgcgcca gaaattggct ctcgcataag cgaccgaatt
     7081 tgcagtaccc ccgttgcaat ggaatgacag cgggtatgtt aaacaacccc atccggcatg
     7141 gagccaggtg gacgttaaat ataaacctga agattaaaca tgactgaatc ttttgctcaa
     7201 ctctttgaag agtccttaaa agaaatcgaa acccgcccgg gttctatcgt tcgtggcgtt
     7261 gttgttgcta tcgacaaaga cgtagtactg gttgacgctg gtctgaaatc tgagtccgcc
     7321 atcccggctg agcagttcaa aaacgcccag ggcgagctgg aaatccaggt aggtgacgaa
     7381 gttgacgttg ctctggacgc agtagaagac ggcttcggtg aaactctgct gtcccgtgag
     7441 aaagctaaac gtcacgaagc ctggatcacg ctggaaaaag cttacgaaga tgctgaaact
     7501 gttaccggtg ttatcaacgg caaagttaag ggcggcttca ctgttgagct gaacggtatt
     7561 cgtgcgttcc tgccaggttc tctggtagac gttcgtccgg tgcgtgacac tctgcacctg
     7621 gaaggcaaag agcttgaatt caaagtaatc aagctggatc agaagcgcaa caacgttgtt
     7681 gtttctcgtc gtgccgttat cgaatccgaa aacagcgcag agcgcgatca gctgctggaa
     7741 aacctgcagg aaggcatgga agttaaaggt atcgttaaga acctcactga ctacggtgca
     7801 ttcgttgatc tgggcggcgt tgacggcctg ctgcacatca ctgacatggc ctggaaacgc
     7861 gttaagcatc cgagcgaaat cgtcaacgtg ggcgacgaaa tcactgttaa agtgctgaag
     7921 ttcgaccgcg aacgtacccg tgtatccctg ggcctgaaac agctgggcga agatccgtgg
     7981 gtagctatcg ctaaacgtta tccggaaggt accaaactga ctggtcgcgt gaccaacctg
     8041 accgactacg gctgcttcgt tgaaatcgaa gaaggcgttg aaggcctggt acacgtttcc
     8101 gaaatggatt ggaccaacaa aaacatccac ccgtccaaag ttgttaacgt tggcgatgta
     8161 gtggaagtta tggttctgga tatcgacgaa gaacgtcgtc gtatctccct gggtctgaaa
     8221 cagtgcaaag ctaacccgtg gcagcagttc gcggaaaccc acaacaaggg cgaccgtgtt
     8281 gaaggtaaaa tcaagtctat cactgacttc ggtatcttca tcggcctgga cggcggcatc
     8341 gacggcctgg ttcacctgtc tgacatctcc tggaacgttg caggcgaaga agcagttcgt
     8401 gaatacaaaa aaggcgacga aatcgctgca gttgttctgc aggttgacgc agaacgtgaa
     8461 cgtatctccc tgggcgttaa acagctcgca gaagatccgt tcaacaactg ggttgctctg
     8521 aacaagaaag gcgctatcgt aaccggtaaa gtaactgcag ttgacgctaa aggcgcaacc
     8581 gtagaactgg ctgacggcgt tgaaggttac ctgcgtgctt ctgaagcatc ccgtgaccgc
     8641 gttgaagacg ctaccctggt tctgagcgtt ggcgacgaag ttgaagctaa attcaccggc
     8701 gttgatcgta aaaaccgtgc aatcagcctg tctgttcgtg cgaaagacga agctgacgag
     8761 aaagatgcaa tcgcaactgt taacaaacag gaagatgcaa acttctccaa caacgcaatg
     8821 gctgaagctt tcaaagcagc taaaggcgag taattctctg actcttcggg atttttattc
     8881 tgaagtttgt tgagtttact tgacagattg caggtttcgt cctgtaatca agcactaagg
     8941 gcggctacgg ccgcccttaa tcaatgcagc aacagcagcc gcttaatttg cctttaagga
     9001 accggaggaa tcatgaccaa gtcagaattg atagaaagac ttgccaccca gcaatcgcac
     9061 attcccgcca agacggttga agatgcagta aaagagatgc tggagcatat ggcctcgact
     9121 cttgcgcagg gcgagcgtat tgaaatccgc ggtttcggca gtttctcttt gcactaccgc
     9181 gcaccacgta ccggacgtaa tccgaagact ggcgataaag tagaactgga aggaaaatac
     9241 gttcctcact ttaaacctgg taaagaactg cgcgatcgcg ccaatattta cggttaagtt
     9301 tttactcaaa cttgaacgag agaaaagcac ctgtcgggtg cttttttcat ttatctaatc
     9361 tggaactgga agctgcctcg cagagttttg agcagttttc accctttcgt taaattcttc
     9421 tgaatgtgcc tcggggaacg caaaattccc accagacaac cgctcaacaa agttgcacac
     9481 tttccataaa cagggagggg tgcatgaaaa taacgacagt cggcgtatgc ataatttgcg
     9541 gaatttttcc gttgctgatt ttgccccaat tgcctgggac attaaccctt gcgtttctga
     9601 ctctcttcgc ctgcgtactg gcatttatcc ctgttaaaac cgtccgttat atcgcgctga
     9661 cgttgctgtt tttcgtttgg ggcatattat cagcaaagca aattttgttg gcaggagaaa
     9721 ccttaactgg cgcgacgcag gatgcaattg ttgagatcac tgctactgac ggcatgacca
     9781 ctcattacgg tcaaattact tatctacaag gtcaacgtat attccctgcg ccaggccttg
     9841 tgctgtatgg cgaatatctt ccgcaagcgg tttgtgccgg acaactgtgg tcaatgaaac
     9901 tcaaagttcg tgccgttcat ggtcaactta atgatggcgg ctttgatagc cagcgttatg
     9961 ccattgccca gcatcagccg ctcaccggcc gctttctgca ggcaagtgtt attgaaccga
    10021 attgtagcct gcgtgcacag tatctggcgt cactacaaac aacgctgcaa ccctatccgt
    10081 ggaatgcggt tattcttggt ttaggtatgg gggaacggtt atccgtccct aaagaaatca
    10141 aaaatatcat gcgtgatact ggaacggcgc atttaatggc gatatcggga ttgcacatcg
    10201 cttttgcggc gttgctggct gccggactca ttcgcagtgg acaaattttt ctgcctgggc
    10261 gctggatcca ctggcaaatg ccattaattg gcggaatctg ctgtgctgct ttttatgcct
    10321 ggctgacggg aatgcaacct cctgcattgc gtaccgttgt ggcgcttgct acgtggggta
    10381 tgcttaagtt aagtgggcga cagtggagtg gctgggatgt atggatatgt tgtctggcgg
    10441 caattttgct gatggatcct gttgtcattc tctcgcaaag tttatggctc tctgccgctg
    10501 cggtcgcggc attgatattt tggtatcagt ggtttccctg tcctgagtgg caactgccgc
    10561 cggtattgcg tgcagttgtt tccctcatcc atctgcaact gggaatcaca cttctgctta
    10621 tgcccgtgca aatcgtcatt tttcatggca ttagtctgac ctcgtttatt gcaaatctat
    10681 tagcaattcc cttggtgaca tttatcacgg ttccgttgat cctcgccgcg atggttgtgc
    10741 atttaagcgg gccgttaatc ctggatcaag ggttatggtt tcttgccgac cggtctttgg
    10801 ctttactttt cttggggtta aagagtttgc cagaagggtg gatcaacatt gctgaacgtt
    10861 ggcaatggct atcattttcc ccatggttct tactggtggt atggcgatta aacgcctggc
    10921 gaacgttgcc agcaatgtgt gtggctgtag gcttgctgat gtgctggccg ctgtggcaaa
    10981 aacctcgacc tgacgagtgg caagtgtaca tgcttgatgt cgggcaaggg ctggcaatgg
    11041 tgatagccag aaacggcaaa gcgattctct atgacacggg actggcctgg cctgaagggg
    11101 atagtgggca acaactgatt atcccctggc tccactggca taatcttgaa ccggaaggcg
    11161 ttatcctgag tcatgaacat ctggatcacc ggggagggct ggactcaata ttgcatacat
    11221 ggccgatgtt atggatcaga agtccgttaa actgggaaca tcatcagccc tgtgtgcgtg
    11281 gcgaagcgtg gcaatggcaa ggattgcgtt tcagcgcgca ctggccttta caaggtcgca
    11341 acgataaagg aaataaccat tcctgtgtgg ttaaggttga tgacgggacg aatagcattc
    11401 ttctaaccgg tgatattgaa gccccagctg aacaaaagat gctaagccgt tactggcagc
    11461 aagtgcaggc aacattgctt caggtacctc accatggcag taatacctca tcatcattgc
    11521 cattaattca gcgagtgaat ggaaaagtgg cactcgcatc ggcatcgcgc tataacgcat
    11581 ggcgactgcc ctctaacaaa gttaagcatc gctatcaaca acaaggttat acgtggcttg
    11641 atactcctca tcaggggcaa gtaacggtcg atttttcagc gcaaggctgg cggattagca
    11701 gcctcaggga gcaaatttta cctcgttggt atcatcagtg gtttggcgtg ccagtggata
    11761 acgggtagaa tatgcggcta tttcaacaaa tgctggtttt ttgaatgcat aacgacaaag
    11821 atctctctac gtggcagaca ttccgccgac tgtggccaac cattgcgcct tttaaagcgg
    11881 gtctgatcgt ggcgggcgta gcgttaatcc tcaacgcagc cagcgatacc ttcatgttat
    11941 cgctccttaa gccacttctt gatgatggct ttggtaaaac agatcgctcc gtgctggtgt
    12001 ggatgccgct ggtggtgatc gggctgatga ttttgcgtgg tatcaccagc tatgtctcca
    12061 gctactgtat ctcctgggta tcaggaaagg tggtaatgac catgcgtcgc cgcctgtttg
    12121 gtcacatgat gggaatgccg gtttcattct ttgacaaaca gtcaacgggt acgctgttgt
    12181 cacgtatcac ctacgattcc gaacaggttg cttcttcctc ttccggcgca ctgattactg
    12241 ttgtgcgtga aggtgcgtcg atcatcggcc tgttcatcat gatgttctat tacagttggc
    12301 aattgtcgat cattttgatt gtgctggcac cgattgtttc gattgcgatt cgcgtagtat
    12361 cgaagcgttt tcgcaacatc agtaaaaaca tgcagaacac catggggcag gtgaccacca
    12421 gcgctgaaca aatgctgaaa ggccataaag aagtattgat tttcggtggt caggaagtgg
    12481 aaacgaaacg cttcgataaa gtcagcaacc gaatgcgtct tcaggggatg aaaatggttt
    12541 cagcctcttc catctctgat ccgatcattc agctgatcgc ctctttggcg ctggcgtttg
    12601 ttctgtatgc ggcgagcttc ccaagtgtca tggatagcct gactgccggt acgattaccg
    12661 ttgttttctc ttcaatgatt gcactgatgc gtccgctgaa atcgctgact aacgttaacg
    12721 cccagttcca gcgcggtatg gcggcttgtc agacactgtt taccattctg gacagtgagc
    12781 aggagaaaga cgaaggtaag cgcgtgatcg agcgtgcgac tggcgacgtg gaattccgca
    12841 acgtcacctt tacttatccg ggacgtgacg tacccgcatt gcgtaacatc aacctgaaaa
    12901 ttccggcagg gaaaacggtc gctctggttg gacgctctgg ttcaggtaaa tcaaccatcg
    12961 ccagcctgat cacgcgtttt tacgatattg atgaaggcga aatcctgatg gatggtcacg
    13021 atctgcgcga gtataccctg gcgtcgttgc gtaaccaggt tgctctggtg tcgcagaatg
    13081 tccatctgtt taacgatacg gttgctaaca acattgctta cgcacgaact gaacagtaca
    13141 gccgtgagca aattgaagaa gcggcgcgta tggcctacgc catggacttc atcaataaga
    13201 tggataacgg tctcgataca gtgattggtg aaaacggcgt actgctctct ggcggtcagc
    13261 gtcagcgtat tgctatcgct cgagccttgt tgcgtgatag cccgattctg attctggatg
    13321 aagctacctc ggctctggat accgaatccg aacgtgcgat tcaggcggca ctggatgagt
    13381 tgcagaaaaa ccgtacctct ctggtgattg cccaccgctt gtctaccatt gaaaaggcag
    13441 acgaaatcgt ggtcgtcgag gatggtgtca ttgtggaacg cggtacgcat aacgatttgc
    13501 ttgagcaccg cggcgtttac gcgcaacttc acaaaatgca gtttggccaa tgatcgaaaa
    13561 aatctggtct ggtgaatccc ctttgtggcg gctattgctg ccactctcct ggttgtatgg
    13621 cctggtgagt ggcgcgatcc gtctttgcta taaactaaaa ctgaagcgcg cctggcgtgc
    13681 ccccgtaccg gttgtcgtgg ttggtaatct caccgcaggc ggcaacggaa aaaccccggt
    13741 cgttgtctgg ctggtggaac agttgcaaca gcgcggtatt cgcgtggggg tcgtatcgcg
    13801 gggatatggt ggtaaggctg aatcttatcc gctgttattg tcggcagata ccaccacagc
    13861 acaggcgggt gatgaacctg tgttgattta tcaacgcact gatgcgcctg ttgcggtttc
    13921 tcccgttcgt tctgatgcgg taaaagccat tctggcgcaa caccctgatg tgcagatcat
    13981 cgtaaccgac gacggtttac agcattaccg tctggcgcgt gatgtggaaa ttgtcgttat
    14041 tgatggtgtg cgtcgctttg gcaatggctg gtggttgccg gcggggccaa tgcgtgagcg
    14101 agcggggcgc ttaaagtcag ttgatgcggt aatcgtcaac ggcggtgtcc cccgcagcgg
    14161 tgaaatcccc atgcatctgc tgccgggtca ggcggtgaat ttacgtaccg gtacgcgttg
    14221 tgacgttgct cagcttgaac atgtggtggc gatggcaggg attgggcatc cgccgcgctt
    14281 ttttgccacg ctgaagatgt gcggcgtaca accggaaaaa tgtgtaccgc tggccgatca
    14341 tcagtctttg aaccatgcgg atgtcagcgc gttggtaagc accgggcaaa cgctggtaat
    14401 gactgaaaaa gatgcggtga aatgccgggc ctttgcagaa gaaaattggt ggtatttgcc
    14461 cgttgacgca cagctttcag gtgatgaacc agcgaaactg cttgcgcaac taacctcgct
    14521 ggcttctggc aactagttac gccgcggcag cgttcgattg atggagtcat gaatgtcgct
    14581 gccgcacctc tcccttgctg atgcgcgtaa tcttcacctt gccgcgcaag gcctgttaaa
    14641 caaaccccgc cgtcgagcgt cgttggagga tattccggca acgatctccc gcatgtcctt
    14701 gctgcaaatc gataccatca atattgttgc ccgtagccca tatctggtgc ttttcagtcg
    14761 tctgggaaat tatcctgccc agtggctgga tgagtctctg gcgcgtggcg aattaatgga
    14821 atactgggcg catgaagcct gctttatgcc acgcagtgac tttcgtctta ttcgccaccg
    14881 catgctggca cctgaaaaaa tgggctggaa atacaaagac gcctggatgc aggaacatga
    14941 ggcggaaatt gcacagttaa ttcagcatat tcatgataag gggccggtac gttcagccga
    15001 ttttgagtat cctcgtaaag gtgcaagcgg ctggtgggaa tggaagccgc ataaacggca
    15061 tctggaaggt ttatttactg ccggaaaggt gatggtgatt gaacggcgca acttccagcg
    15121 cgtttatgat ttaacccacc gtgtcatgcc tgactgggat gatgagcgcg atctcgtttc
    15181 gcaaacagaa gcagaaatca tcatgctgga taacagtgcg cgtagccagg gaatattccg
    15241 cgaacagtgg ctggcagatt actatcggct gaaacgtccg gcactggcag cgtggcgcga
    15301 agcgagggct gaacagcggc aaatcattgc tgtgcatgtt gaaaaattgg gcaatctttg
    15361 gctgcatgct gatttgctgc cgctactcga gcgagcgctg gccggaaagc tcactgcaac
    15421 gcacagcgcg gtactttcgc cttttgatcc tgttgtctgg gatcgcaaac gcgcagagca
    15481 gctttttgat tttagctacc ggctggagtg ctatacccca gcgccgaaac gccagtatgg
    15541 ctattttgtt ctgccgttat tacatcgtgg gcaattagtt gggcgaatgg atgccaaaat
    15601 gcatcgccag acaggcatcc ttgaagttat ctctctgtgg ttacaggaag gtattaaacc
    15661 aacgacaacg ctgcaaaaag ggttacgtca ggcgattact gatttcgcta actggcagca
    15721 ggcaacgcgg gtgacattag gacactgccc gcaaggtctc tttacggatt gccgcaccgg
    15781 ctgggaaata gaccccgtcg cataaatgat tatgataaga tttatgcatt cataacccga
    15841 tccatctgga ggaactatgg atcatcgtct gcttgaaatc attgcctgcc cggtttgcaa
    15901 cggaaaactt tggtataacc aggaaaaaca agaacttatt tgcaaactgg acaacctggc
    15961 tttcccgctg cgcgatggca ttccagtgtt gctggaaacg gaagcccgcg tgctgactgc
    16021 tgatgagagt aaatcatgag ttttgtggtc attattcccg cgcgctacgc gtccacgcgt
    16081 ctgcccggta aaccattggt tgatattaac ggcaaaccca tgattgttca tgttcttgaa
    16141 cgcgcgcgcg aatcaggtgc cgagcgcatc atcgtggcaa ccgatcatga ggatgtcgcc
    16201 cgcgccgttg aagccgctgg cggtgaagta tgtatgacgc gcgccgatca ccagtcaggg
    16261 acagaacgct tggcggaagt tgtcgaaaaa tgcgcgttca gcgacgacac ggtgatcgtt
    16321 aatgtgcagg gtgatgaacc gatgatccct gcgacaatca ttcgtcaggt tgctgataac
    16381 ctcgctcagc gtcaggtggg tatggcgact ctggcggtgc caatccacaa tgcggaagaa
    16441 gcgtttaacc cgaatgcggt gaaagtggtt ctcgacgctg aagggtatgc actgtacttc
    16501 tcacgcgcta ccattccgtg ggatcgtgat cgttttgcaa aaggccttga aaccgttggc
    16561 gataacttcc tgcgtcatct tggtatttat ggctaccgtg caggctttat ccgtcgttac
    16621 gtcacctggc agccaagccc gttagaacac atcgaaatgt tagagcagct tcgtgttttg
    16681 tggtacggcg aaaaaatcca tgttgctgtc gcgcatgaag ttcctggcac aggtgtggat
    16741 acccctgaag atctcgagcg cgttcgcgct gaaatgcgct aatttcactt cacgacactt
    16801 cagccaattt tgggaggagt gtcgtaccgt tacgattttc ctcaattttt cttttcaaca
    16861 attgatctca ttcaggtgac atcttttata ttggcgctca ttatgaaagc agtagctttt
    16921 atgagggtaa tctgaatgga acagctgcgt gccgaattaa gccatttact gggcgaaaaa
    16981 ctcagtcgta ttgagtgcgt caatgaaaaa gcggatacgg cgttgtgggc tttgtatgac
    17041 agccagggaa acccaatgcc gctaatggca agaagcttta gtacgcccgg aaaagcccga
    17101 caactggcat ggaaaaccac catgctggca agaagtggga ctgtccgtat gccgactatt
    17161 tatggtgtga tgacgcatga agaacacccc ggccctgatg tcctgctact ggagcggatg
    17221 cgtggtgttt cggtagaggc accagcccga acaccagaac gttgggaaca gctcaaagac
    17281 caaatcgttg aagccttact ggcctggcac cgtcaggaca gtcgcggttg cgtcggcgcg
    17341 gtcgacaata ctcaggaaaa tttctggccc tcatggtacc ggcaacatgt tgaagtgcta
    17401 tggaccacgc tcaatcagtt caataacacc ggtctgacga tgcaggataa gcggatcctg
    17461 tttcgcactc gcgaatgtct cccggcatta tttgaaggtt ttaacgacaa ttgtgtgctg
    17521 attcacggta acttctgttt acgcagcatg ttgaaagatt cgcgcagcga tcagttactg
    17581 gcgatggtcg ggccgggact aatgctttgg gcaccgcgag aatacgaact gttccgacta
    17641 atggataatt ctctggcgga agatttgctc tggagttacc tgcaacgcgc gccagtggcg
    17701 gagtcgttca tctggcggcg ttggttgtat gtgttatggg atgaagttgc gcaactggtt
    17761 aataccggac gatttagtcg gcgcaacttc gatctggcat caaaatcact cttgccgtgg
    17821 ctcgcctgac gaacctttta gccactgcca gatgcggcca agcgtttcat agccaacacg
    17881 atcgctatgc atcaaccaga ccggagaagg gattgcccgt tcccacgggt taagcggcga
    17941 gtcgatggcc aactgattcg ccggggctgg tagtggattt aacccttcct gctgaaaaaa
    18001 gatcatcgcc cgcggcagat gtgatgcgga ggtcaccagc aggaaagggg cgtcaccaat
    18061 cgcctgtttc actgccgcag cttcttcttc ggtatctttt ggcaaatcca gggtgataat
    18121 ttgctcgcgc ggtacgccca gcgattgcgc aactctggca cctacttccg ctgtactcac
    18181 cgtattggtt tttgctacgc ctcccgtgaa gatcagtttt gaacccggat tttcgcgcca
    18241 taaacggata ccctcattca ggcgtggcag actgttattg attaaattag agctcggtgc
    18301 ccactgcggg ttccaggtat aaccacctcc gagcaccacg atatagtcca ctttttgcga
    18361 attattccat gtcggatagg tgctttcgat gggacgcagc aggcggtcgg ctaccggttg
    18421 caaactcaat aacaagagtg ccagccaccc aatgctaata aaaatcttcc cggttttttg
    18481 aaaacggcta aaccacagta gcgccaggcc agcgccaata attaacaaca tcagcggaag
    18541 tggcagcaac atattgccaa tcactttttt cagtgtaaaa agcatccttt ctggttcctt
    18601 ttttaaccat atagcaaggg atcgccagcg atattacacc agacgggttc attctcgccg
    18661 tggctgtgac aaaatagcgt ctttatcaga aaacgtctta gccaaccggt ggagaaagca
    18721 atgcaggatc gcaattttga tgatattgcg gaaaagtttt cccgtaacat ttacggcacc
    18781 accaaagggc agcttcgaca ggctattctg tggcaggatc tcgatcgcgt gctggcggaa
    18841 atgggtccgc aaaaactgcg tgtgctggat gcgggcggtg gagaagggca gaccgcaatc
    18901 aaaatggccg agcgcgggca tcaggtcatt ttatgcgatc tttctgcgca gatgatcgac
    18961 cgcgcaaaac aggcggcaga agcaaaaggt gtgagcgaca acatgcaatt tatacattgc
    19021 gccgctcagg atgttgcttc gcatttggaa acgcccgttg atctgatatt gttccatgcg
    19081 gtgctcgagt gggtggctga tccccgcagc gtattgcaga ccctgtggtc agtgttgcgt
    19141 ccaggcggcg tgttgtcgtt aatgttctac aatgcgcatg gtttgttgat gcataacatg
    19201 gtcgccggga attttgatta cgtgcaggcg ggaatgccga aaaagaaaaa acggacgctt
    19261 tcgccagatt atccacgcga cccgacgcag gtttatctgt ggctggaaga agctggttgg
    19321 caaattatgg gtaagacagg cgttcgcgtg tttcatgatt atctgcgcga gaagcaccag
    19381 cagcgcgact gctatgaagc attacttgaa ttagaaacgc gttattgccg tcaggaaccg
    19441 tatattaccc tggggcgtta tattcatgtc accgcgcgca aaccgcagag caaggataaa
    19501 gtatgagtga attttcccag acagtccccg aactggttgc ctgggccaga aaaaatgact
    19561 tctccatttc gctgccggta gaccgactct cttttctgct ggcagttgcc acgctgaacg
    19621 gcgagcgtct ggatggtgag atgagtgaag gcgagctggt ggatgcattc cgccatgtga
    19681 gtgatgcgtt tgagcaaacc agcgaaacca tcggcgtgcg cgccaacaac gcgatcaacg
    19741 acatggtgcg tcaacgtctg ctgaaccgct ttaccagcga gcaggcagaa gggaacgcaa
    19801 tttaccgtct gacgccgctc ggcatcggca ttactgacta ttacatccgt cagcgcgagt
    19861 tttctacgct gcgcctttct atgcagttgt cgattgtggc gggtgagctc aaacgcgcag
    19921 cagatgccgc cgaagagggc ggtgatgaat ttcactggca ccgtaatgtc tatgcgccac
    19981 tgaaatattc ggtagcagaa attttcgaca gtatcgacct gacgcaacgt ctgatggacg
    20041 aacagcagca gcaggtgaag gacgatatcg cccagttgct gaacaaagac tggcgggcgg
    20101 cgatttccag ctgtgaattg ttgctttcgg aaacttccgg aacgctgcgt gaattgcagg
    20161 atacactgga agcggcaggc gacaaattgc aggctaatct gttgcgcatt caggatgcga
    20221 cgatgactca tgacgatctg catttcgtcg atcgtctggt gttcgatctg cagagcaaac
    20281 tcgaccgtat tatcagttgg ggccagcaat ccatcgattt gtggattggc tacgaccgcc
    20341 acgtacataa atttattcgt accgcgatcg atatggataa aaaccgcgtc tttgctcagc
    20401 ggttacgtca gtcggtacaa acctattttg atgagccgtg ggcgctaagt tatgccaatg
    20461 ccgatcgtct gctggatatg cgtgacgaag agatggcact gcgcgatgaa gaagtgactg
    20521 gggaacttcc gccagatttg gaatacgaag agtttaacga gatccgcgaa cagctggcgg
    20581 cgatcatcga agaacaactt gccgtgtaca aaaccagaca agtgccgctg gatcttggtc
    20641 tggtggtacg cgaatatctg tcacagtatc cgcgtgcacg tcactttgac gttgcgcgca
    20701 ttgttattga tcaggcggta cgtcttggcg tagcgcaagc agatttcacc ggactgccag
    20761 cgaaatggca gccgattaat gattacggag ccaaggtaca ggcgcatgtc atcgacaaat
    20821 attgaacaag tgatgccggt taagctggcg caggcgctgg cgaatccgtt atttccggcg
    20881 ctggacagcg ccttacgttc aggccgccat attggcctcg acgaactgga taatcatgca
    20941 ttcctgatgg attttcagga atatctggaa gagttttacg cgcgttataa cgttgagctt
    21001 attcgtgcac cggaagggtt cttctattta cgtccacgtt ccaccacgct gatccctcgt
    21061 tccgtcttgt cggaactgga tatgatggtc gggaaaatcc tctgttatct ctatctcagc
    21121 ccggaacggc tggcgaatga ggggattttc acccagcagg aactgtacga cgaactgctc
    21181 accctggccg atgaagcgaa actgctgaaa ctggtgaaca accgttcaac cggttcagac
    21241 attgaccgtc agaagttgca ggaaaaagta cgttcttcgc tcaaccgtct gcgtcgttta
    21301 ggtatggtgt ggtttatggg ccacgacagc agcaagttcc gcattaccga atcggtgttc
    21361 cgcttcggag ccgatgtgcg tgctggcgac gatccccgtg aagcacagcg tcgcctgatt
    21421 cgtgatggcg aagcaatgcc gattgaaaat catctgcaac tcaacgatga aaccgaagag
    21481 aatcagccag atagcggaga ggaagaataa tgattgaacg cggtaaattt cgctcactga
    21541 cgctgattaa ctggaacggc ttctttgccc gaacttttga ccttgacgag ctggtcacga
    21601 cgctttctgg cggtaacggg gcgggtaaat ccaccaccat ggcggcgttc gttacggcgc
    21661 tgatccccga cctgaccctg ctgcatttcc gtaacactac ggaagccggg gccaccagcg
    21721 gttcgcgcga taaaggtctg cacggtaagc tgaaagcggg tgtctgttat tcgatgctcg
    21781 acaccattaa ctcgcgccac cagcgcgtgg tggtcggtgt gcgtctgcaa caggttgccg
    21841 gacgcgatcg taaagtggat atcaaaccat ttgccattca gggactgccg atgtcggtgc
    21901 agccgacaca gctggtgacc gaaactctga acgaacgcca ggcgcgcgtg ctgccgctta
    21961 acgagctgaa agacaagctc gaggcgatgg aaggcgtgca gtttaaacag ttcaactcca
    22021 ttaccgatta ccactcgctg atgttcgatc tgggcatcat tgcgcgtcgt ctgcgctccg
    22081 catctgaccg tagcaaattc tatcgtctga tcgaagcttc gctgtatggc gggatctcca
    22141 gtgccattac ccgttctctg cgcgactacc tgttgccgga aaacagcggc gtgcgtaaag
    22201 cgttccagga catggaagcg gcgctgcgtg aaaaccgcat gacgctggaa gcgattcgtg
    22261 tcacccagtc ggaccgtgac ctgtttaagc atctgatcag cgaagctacc aactacgtgg
    22321 cggcggacta catgcgtcac gccaacgagc gccgtgtcca tctcgataag gccctggagt
    22381 ttcgtcgcga actacatact tcgcgtcagc aactggcggc ggagcagtac aaacacgtcg
    22441 atatggcgcg tgagctggca gagcacaacg gtgccgaagg cgatctggaa gcggattatc
    22501 aggcggccag cgatcacctg aacctggtgc aaaccgcact gcgtcagcag gagaagatcg
    22561 aacgctacga agcggatctc gatgagctgc agatccgtct ggaagagcaa aacgaagtgg
    22621 tggcagaagc catcgaacgc caggaagaga acgaggctcg cgcggaagct gccgaactgg
    22681 aagtggacga gctgaaaagc cagcttgctg actaccagca ggcgctggac gtccagcaaa
    22741 cgcgcgcgat ccagtacaac caggcgattg ctgcgcttaa tcgtgccaaa gaactgtgcc
    22801 atctgccgga cttaaccgcc gacagcgccg ccgaatggct ggaaaccttc caggcgaaag
    22861 agctggaagc gactgaaaaa atgctctctc ttgagcagaa aatgagcatg gcgcaaaccg
    22921 cgcacagcca gtttgagcag gcttatcagc tggtggtggc aatcaacggc ccgctggcgc
    22981 gtaacgaggc gtgggatgtc gctcgcgaac tattgcgcga aggggtcgat cagcgtcacc
    23041 tggcagagca ggttcagccg ttgcggatgc gcttaagcga actggaacag cgtctgcgcg
    23101 aacagcaaga agctgagcgt ctgctggcag atttctgcaa acgtcagggc aagaattttg
    23161 acattgacga gctggaagcc ctgcatcagg agctggaagc acgcattgcc tctctttccg
    23221 atagcgtgtc taacgcccgt gaagagcgga tggcactgcg ccaggagcag gaacagctgc
    23281 agtctcgcat tcagagtttg atgcagcgtg caccggtttg gctggcagcg caaaacagtc
    23341 tcaaccagtt gagcgaacag tgcggcgaag agttcacctc cagccaggac gtcactgaat
    23401 atctgcaaca gttgctggag cgtgagcgcg aggccattgt tgaacgcgat gaagtaggcg
    23461 cgcgcaaaaa cgccgtcgat gaagagatcg aacgtttaag ccagcctggc ggctctgaag
    23521 atcagcgttt gaacgcgcta gcggagcgtt ttggtggtgt gctgctgtca gaaatttatg
    23581 acgacgttag cctggaagat gcgccgtact tctcagcgct gtatggcccg tcacgccacg
    23641 ccatcgtggt gccagatctg tcacaggtaa ccgaacacct ggaaggtttg accgattgcc
    23701 cggaagatct ctatttgatc gaaggggatc cgcagtcatt cgatgacagc gtgttcagcg
    23761 ttgatgagct ggaaaaagcg gtagtggtga aaatcgccga tcgtcagtgg cgttattcac
    23821 gtttcccgga agtgccgctg tttggtcgtg ctgcgcgtga aagccgtatt gaaagcctcc
    23881 atgccgagcg tgaagtgctt tccgaacgct tcgccacgct ctcctttgat gtacagaaaa
    23941 cccagcgtct gcatcaggcg ttcagccgct ttatcggcag tcatctggcg gtggcgtttg
    24001 agtctgaccc ggaagcagaa atccgtcaac tgaacagccg tcgcgtcgaa ctggagcggg
    24061 cgttgagtaa tcatgaaaat gataaccagc agcagcgtat tcagtttgag caggcgaaag
    24121 aaggcgttac ggcgctgaac cgcattctgc cgcgtctcaa cctgttggct gatgacagcc
    24181 tggcggatcg cgtcgatgaa atccgcgaac gtctggatga agcccaggaa gccgcgcgtt
    24241 ttgttcagca gtttggcaat caactggcga aactggaacc gatcgtttcg gtattgcaga
    24301 gcgacccgga acagttcgaa cagttaaaag aagattacgc gtactctcag cagatgcagc
    24361 gcgatgcccg tcagcaggcg tttgccctga cggaagtggt gcagcgtcgt gcgcacttta
    24421 gctactctga ctcggcagaa atgcttagcg gtaacagcga tctcaacgaa aaactgcgtg
    24481 aacgtctgga acaggcggaa gcggagcgta cccgcgctcg cgaagcgttg cgcggtcacg
    24541 cagcgcagtt gagtcagtac aaccaggtgc tggcttcgct gaaaagttct tacgacacca
    24601 aaaaagagct actcaacgat ctgcaacgtg aattgcagga tatcggcgtg cgtgctgata
    24661 gcggggcaga agagcgggca cgtattcgcc gtgacgagct gcatgcacaa ctgagcaata
    24721 accgttcgcg ccgcaatcaa ctggaaaaag cgctcacctt ctgcgaagcg gagatggata
    24781 acctgacccg caaactgcgc aagctggagc gtgattactt tgagatgcgc gagcaggtag
    24841 tgaccgcgaa agcgggctgg tgtgcagtca tgcgcatggt gaaagataac ggcgttgagc
    24901 gccgcttaca ccgtcgtgag ctggcttatc tctccgctga tgatttgcgt tccatgtcgg
    24961 ataaggcgtt aggtgcgctg cgtctggcgg tggcggataa cgaacatctg cgcgacgtgc
    25021 tgcgcatgtc ggaagatccc aaacgtccgg agcgtaaaat tcagttcttc gtggcggttt
    25081 atcagcatct gcgtgaacgt attcgtcagg atattattcg taccgatgat ccggtggaag
    25141 ctatcgaaca gatggagatc gaacttagcc gtctgaccga agagttaacc tcccgtgaac
    25201 agaaactggc gatcagttcc cgcagcgtgg cgaacatcat tcgcaaaacc attcagcgcg
    25261 agcagaaccg tatccgtatg ctcaaccagg ggttgcagaa cgtatcgttt ggtcaggtga
    25321 acagcgtgcg cctcaacgtg aacgtgcgtg aaacgcacgc catgctgctg gatgtgctct
    25381 ctgaacagca tgagcagcat caggatctgt ttaacagcaa ccgtctgacc ttctcggaag
    25441 cgctggcgaa attgtatcag cgtcttaatc cgcagattga tatggggcag cgcacgccgc
    25501 agaccattgg cgaagaactg ctggattacc gcaactatct ggaaatggaa gttgaggtta
    25561 accgtggttc cgatggctgg ttgcgcgcag agtctggtgc attgtcgacc ggtgaggcga
    25621 ttggtaccgg tatgtcgatt ctggtgatgg tggtacaaag ctgggaagat gaatctcgcc
    25681 gcctgcgcgg taaagatatc tctccttgcc gcctgctgtt cctcgatgaa gcagcgcgac
    25741 tggatgctcg ttctatcgcc acgctgtttg aattgtgtga gcgtttgcaa atgcagctca
    25801 tcatcgcagc gccggaaaat atcagcccgg agaaaggcac cacctataaa ctggtgcgta
    25861 aagtcttcca gaataccgaa catgttcatg tcgtcggcct gcgaggattt gcgccgcaac
    25921 tccctgaaac gcttccagga agtgacgaag cgccttctca ggcgagttaa aattaaggcg
    25981 gcagcaatgc cgccttttct ttttccgaaa actccgtttc tgcactaaaa aagtggcaca
    26041 ttacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
    26101 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
    26161 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
    26221 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
    26281 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
    26341 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
    26401 ggcaataatg gcggggatcc tgccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
    26461 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
    26521 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
    26581 gcaacagctg gcagaggtgg cgattgccgg tttccagccg cagtttaata aatgggtaga
    26641 gttactgacc gatcctggtg ttaacgggat ggcacgcgac gtggtgctct ctgatgcgat
    26701 gatgggctat ctccatttca ttgcaaatat tccggtcaaa ggcactcgct ggctatatag
    26761 cagtaaacct tatgcgcttg caacgccgcc gctttcggtg attaaccaat ggcagcaggc
    26821 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
    26881 tgcggtgatg catgaatcgt tactggcctt actcagtgac accaaaccgt ggccccaact
    26941 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgtga
    27001 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
    27061 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
    27121 gccgatggat aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gcgccgccta
    27181 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggggcaga
    27241 tggtgctatt ggcccggcaa cgcgtgactg gttaaacgta acgcccgccc agcgtgctgg
    27301 cgtgttggct ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
    27361 catggttaac attccggcct attcgctggt ctactatcag aacggcaatc aggtgctgga
    27421 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcccttaa
    27481 caatgtagtg gtaaacccgc cgtggaacgt acctccaact ctggcacgca aagatattct
    27541 gccgaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
    27601 ctggaacagc agagaagcga ttgacccatg gcaggttgac tggtctacaa tcacggcctc
    27661 gaatttaccg tttcgcttcc aacaggctcc aggcccacgg aactcgctgg ggcgctataa
    27721 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
    27781 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
    27841 ggcgaatatg ctgttgcagg atgcaggctg gaatgacaaa cgtatttctg atgcgctgaa
    27901 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
    27961 gacggccttt gttggtgcag atggtcgtac ccagtatcgt acagatattt acaattatga
    28021 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
    28081 gaagtagttc ggggaaatca gttgtcgtaa taattagcat gattgggggc aattctctgc
    28141 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
    28201 cctcttgtgc gccagaagtg catataaacg ataacattga cctgtagact tgattatcat
    28261 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
    28321 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
    28381 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
    28441 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
    28501 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tattaggcac
    28561 ccgcaaaccg gtgcaactca tttccggtta tcgttccatt gataccaaca atgaactacg
    28621 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
    28681 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
    28741 tggtgtagga tattacccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
    28801 ctggtagtaa ttgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
    28861 gcattctccc agaactgttc attgatctgg tgtgaacaaa cccgcctggc cgcactggtc
    28921 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg acgccagcgg cctgacactg
    28981 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
    29041 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
    29101 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt
    29161 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
    29221 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
    29281 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
    29341 caactgattt cttcaatcaa agataaattg ctgccactgg gtgatgacgt gacatttatt
    29401 ccgggtcacg gaccattatc cacacttggt tatgaacgcc tgcataatcc cttcctgcaa
    29461 gacgaaatgc ccgtctggta aggcacacat aaaaaagccc gcttttcagc gggcttcatt
    29521 gtttttaacg cttacagcac tgccacaatc gcttcgcaca gcggagccat gttatctggt
    29581 gtcatcccgg ccacgtttac gcgaccagaa gcaacagcat acacgccaaa ctcttcgcgc
    29641 agacgcagca cttgttcttt tgtcaggcca ctgaaggaga acatgccgtt ctgtttgatg
    29701 ataaagctga agtcgcggtt tgcgcctttt tcctgcagcg tattgacgaa caactgacgc
    29761 atacgctgaa tacgctggcg catatcagtc agctcttgtt cccaaatcgc acgtaacgca
    29821 tcgttgctca ggatggtggc aacaacagaa gcgccgtgtg ctggtgggtt agagtagtta
    29881 gcgcgaatcg ccgctttcat ttggctgaat gcgcgatcaa cggtttcact gtcggcagca
    29941 accagagtac aagcgccaac acgctcgttg tacaggccaa agtttttaga gtaggaactg
    30001 gcaacaatca gctctttatg catagccgcg aaagcgcgca gtccttcagc atcttcttcc
    30061 agaccacggg caaaaccctg gtaagcgaag tcaaacagcg gtaaccagcc tttctcaacg
    30121 gagagttgtg ccagtgtctg ccattgttcc agcgtagggt cgataccggt tgggttgtgg
    30181 cagcagccat ggaacagcac tacgtcgcca gcctgagctt cgttcaggct gttaatcagt
    30241 gcatcgaagt caagggtgtg gttttccgca tcataataag cgtattcacg aacttccaga
    30301 tctgcagagt taaagacgct cttatggttc ggccagcttg ggttgctcac ccacactcgc
    30361 ttaacgctgg tattttttgc caggaaatcg gcagctatgc gtagtgcgcc agtgccaccc
    30421 ggagtctgtg ccgtgcgagc acgtttgtca ttgatcaggg cgctaccttt accaaacagc
    30481 agttcctgag tgcagcgacc aaattcaggg atgccgtcaa tgccgaggta atttttggtg
    30541 gtttcatttt cgagcagata ctgttcagcc tttttcacgc tggtcagtac cggggttttg
    30601 cccgtctcat ctttatagac accaatcccg aggttaattt tgccgggacg ttcatcggca
    30661 cgaaacagat cggccaggcc cagaatcggg tcggcaggag cggcggtaat gttctcaaac
    30721 atgacgaggt tccattatgg ttacagaagg gaagtccgct atcagggtaa cgggagattt
    30781 acaaaattcc aactattact gatgaaaacg caggctgttt ttgcaagacg tgagattgct
    30841 ctggaaggca taaaaaaaac aggaccaaag tcctgttttt tcggcattta acaaagaggt
    30901 gtgctattag aactggtaaa cgatacccac agcaacggtg tcgtctgaac ctacgcccag
    30961 tttgttgtca gaatcgatct ggttgatgat gtagtcaaca taggtggaca tgtttttgtt
    31021 gaagtagtag gttgcgccca cttcaaagta gttcaccaga tcaacatcac cgataccttc
    31081 tacgtctttc gctttagatt tggtgtaagc gatggacgga cgcagaccga aatcgaactg
    31141 gtattgcgca actaacagaa cgtcttgcgt tttgttggcg aagccgctga tgtttgtaaa
    31201 tttattagtg atcggcgtag cgttacgcgt ttcaccgtag ttcgctgcca ggtagatgtt
    31261 gttcgcatca tacttcagac cagtagccca ctgttcagct tttttaccgt tgccaagaag
    31321 ttgagcttct tgcaggttgg tacggtcagc tgcaccataa gcaccaacga taccaaagcc
    31381 ttcgtattcg tagctgatag aaccgccaac accgtcgccg ttagagcggc gtgcagtgtc
    31441 acgctcgttt ttacccaggt actgaacagc gaagttcagg ccatcaacca gaccaaagaa
    31501 gttggagtta cgataggtag caacgccgcc aacacgacca acgaagaagt catcgctgta
    31561 tgcagtatca ccaccaaatt ctggcagcat atcggtgtaa cccagtgcat cataaaccac
    31621 accgtagtta cggccgtaat cgaaagaacc aacgtcagcg tatttaagac ccgcgaatgc
    31681 cagacgcgtt ttgttaccag tttgagcgtc agcgccttca gagttgttac cctggaagtt
    31741 atattcccac tgaccataac cggtcagatc ggaattgatt tgagtttccc ctttaaaacc
    31801 aagacgggca taggtcatgt cgccattgcc accgtaactg ttttcaccgt tacccttgga
    31861 gaaataatgc agaccgacag ctttaccgta cagatctact ttgttgccat ctttgttata
    31921 gatttctgca gcgtttgcag tacctgctac taacagagca gggacgatca ctgccagaat
    31981 attgcgcttc atcattattt attaccctca tggttttttt tatgacacct gccactgccg
    32041 tcaataattt ctgtcaataa aattttacgg aactattgat gagagtttgg tgtctt
//
LOCUS       DBFIFI010000035        32069 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.035, whole genome shotgun sequence.
ACCESSION   DBFIFI010000035 DBFIFI010000000
VERSION     DBFIFI010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32069
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.035"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..205
                     /locus_tag="N7B39_001536"
     CDS             <1..205
                     /locus_tag="N7B39_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2421542.1"
                     /translation="TILVLFILSLTTTLNLRSIGIGLSFGFCQILGRYTMSLGWSKYW
                     GVLGGFPVTNIVLFLILIFFKKR"
     gene            complement(252..1139)
                     /gene="rarD"
                     /locus_tag="N7B39_001537"
     CDS             complement(252..1139)
                     /gene="rarD"
                     /locus_tag="N7B39_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HGU2421543.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPTDEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(1191..1658)
                     /gene="yigI"
                     /locus_tag="N7B39_001538"
     CDS             complement(1191..1658)
                     /gene="yigI"
                     /locus_tag="N7B39_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HGU2421544.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1823..2692
                     /gene="pldA"
                     /locus_tag="N7B39_001539"
     CDS             1823..2692
                     /gene="pldA"
                     /locus_tag="N7B39_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HGU2421545.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2825..4654
                     /gene="recQ"
                     /locus_tag="N7B39_001540"
     CDS             2825..4654
                     /gene="recQ"
                     /locus_tag="N7B39_001540"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HGU2421546.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4718..5338
                     /gene="rhtC"
                     /locus_tag="N7B39_001541"
     CDS             4718..5338
                     /gene="rhtC"
                     /locus_tag="N7B39_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HGU2421547.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5400..6020)
                     /gene="rhtB"
                     /locus_tag="N7B39_001542"
     CDS             complement(5400..6020)
                     /gene="rhtB"
                     /locus_tag="N7B39_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HGU2421548.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6131..7153
                     /gene="pldB"
                     /locus_tag="N7B39_001543"
     CDS             6131..7153
                     /gene="pldB"
                     /locus_tag="N7B39_001543"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HGU2421549.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     IHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7161..7961
                     /gene="yigL"
                     /locus_tag="N7B39_001544"
     CDS             7161..7961
                     /gene="yigL"
                     /locus_tag="N7B39_001544"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HGU2421550.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            8037..8936
                     /gene="bioP"
                     /locus_tag="N7B39_001545"
     CDS             8037..8936
                     /gene="bioP"
                     /locus_tag="N7B39_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HGU2421551.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8824..9777)
                     /gene="metR"
                     /locus_tag="N7B39_001546"
     CDS             complement(8824..9777)
                     /gene="metR"
                     /locus_tag="N7B39_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HGU2421552.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            10013..12274
                     /gene="metE"
                     /locus_tag="N7B39_001547"
     CDS             10013..12274
                     /gene="metE"
                     /locus_tag="N7B39_001547"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HGU2421553.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPARHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLDEVDEALALGHKVKPV
                     LLGPITWLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYVQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSMTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12314..13129)
                     /gene="ysgA"
                     /locus_tag="N7B39_001548"
     CDS             complement(12314..13129)
                     /gene="ysgA"
                     /locus_tag="N7B39_001548"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HGU2421554.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGLDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAASAEDGWQRMLEWFKQYGGKKSL"
     gene            13391..14152
                     /gene="udp"
                     /locus_tag="N7B39_001549"
     CDS             13391..14152
                     /gene="udp"
                     /locus_tag="N7B39_001549"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HGU2421555.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            14293..15720
                     /gene="rmuC"
                     /locus_tag="N7B39_001550"
     CDS             14293..15720
                     /gene="rmuC"
                     /locus_tag="N7B39_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HGU2421556.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            15815..16570
                     /gene="ubiE"
                     /locus_tag="N7B39_001551"
     CDS             15815..16570
                     /gene="ubiE"
                     /locus_tag="N7B39_001551"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HGU2421557.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            16584..17189
                     /gene="ubiJ"
                     /locus_tag="N7B39_001552"
     CDS             16584..17189
                     /gene="ubiJ"
                     /locus_tag="N7B39_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HGU2421558.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLAALIRSGELEVQGDI
                     QVVQNFVALAGLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALIKRLEKLEAK"
     gene            17186..18826
                     /gene="ubiB"
                     /locus_tag="N7B39_001553"
     CDS             17186..18826
                     /gene="ubiB"
                     /locus_tag="N7B39_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HGU2421559.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            18905..19174
                     /gene="tatA"
                     /locus_tag="N7B39_001554"
     CDS             18905..19174
                     /gene="tatA"
                     /locus_tag="N7B39_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HGU2421560.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            19178..19693
                     /gene="tatB"
                     /locus_tag="N7B39_001555"
     CDS             19178..19693
                     /gene="tatB"
                     /locus_tag="N7B39_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HGU2421561.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            19696..20472
                     /gene="tatC"
                     /locus_tag="N7B39_001556"
     CDS             19696..20472
                     /gene="tatC"
                     /locus_tag="N7B39_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HGU2421562.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            20514..21296
                     /gene="tatD"
                     /locus_tag="N7B39_001557"
     CDS             20514..21296
                     /gene="tatD"
                     /locus_tag="N7B39_001557"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HGU2421563.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(21293..21781)
                     /gene="rfaH"
                     /locus_tag="N7B39_001558"
     CDS             complement(21293..21781)
                     /gene="rfaH"
                     /locus_tag="N7B39_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HGU2421564.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            21948..23441
                     /gene="ubiD"
                     /locus_tag="N7B39_001559"
     CDS             21948..23441
                     /gene="ubiD"
                     /locus_tag="N7B39_001559"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HGU2421565.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPIS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGEMAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            23487..24188
                     /gene="fre"
                     /locus_tag="N7B39_001560"
     CDS             23487..24188
                     /gene="fre"
                     /locus_tag="N7B39_001560"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HGU2421566.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(24380..25543)
                     /gene="fadA"
                     /locus_tag="N7B39_001561"
     CDS             complement(24380..25543)
                     /gene="fadA"
                     /locus_tag="N7B39_001561"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HGU2421567.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSVSDIGVFEMNEAFAAQILPCIKDLGLIEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(25553..27742)
                     /gene="fadB"
                     /locus_tag="N7B39_001562"
     CDS             complement(25553..27742)
                     /gene="fadB"
                     /locus_tag="N7B39_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HGU2421568.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVIMKDINDKSLALGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFER
                     VDVVVEAIVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWHYKEDSKGKPKKEEDAAVDDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            27932..29263
                     /gene="pepQ"
                     /locus_tag="N7B39_001563"
     CDS             27932..29263
                     /gene="pepQ"
                     /locus_tag="N7B39_001563"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HGU2421569.1"
                     /translation="MESLASLYKNHIATLQERTRDALTRFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQASEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            29263..29877
                     /gene="yigZ"
                     /locus_tag="N7B39_001564"
     CDS             29263..29877
                     /gene="yigZ"
                     /locus_tag="N7B39_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HGU2421570.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITLLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            29916..31367
                     /gene="trkH"
                     /locus_tag="N7B39_001565"
     CDS             29916..31367
                     /gene="trkH"
                     /locus_tag="N7B39_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HGU2421571.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            31379..31924
                     /gene="hemG"
                     /locus_tag="N7B39_001566"
     CDS             31379..31924
                     /gene="hemG"
                     /locus_tag="N7B39_001566"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HGU2421572.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         7452 a         8133 c         8760 g         7724 t
ORIGIN      
        1 taccatcctt gttctattta ttttaagcct gactacgaca ttaaatttaa gaagtatagg
       61 tatagggtta agttttgggt tttgtcaaat ccttggtaga tatacgatgt ctctaggatg
      121 gagtaagtac tggggagttt tggggggatt tcctgtaaca aatattgttc tttttcttat
      181 cttaattttt ttcaaaaaaa gataaataag tcagtgggta ggctctttaa acggcctacc
      241 catgttaatt cttaactttt tctacgctgc gtataaatcg catccatcac aaaaattgcc
      301 agcgccaccc aaataaaggt gaaagtcacc atcttatcgg cacccggttt ttcaccataa
      361 aacgtcacag ccagcaggaa catcagcgtc gggccaatgt actggaaaaa gcctaacgtt
      421 gagagacgaa ggcgcgtggc agcggcggta aaacacaaca gcggtacggt agtgacaata
      481 ccggcggcga tcagcagtaa attcagcgac atcgggtttt gccccatatg gctggttgag
      541 ctgtcggcaa tagcaaacag gtaaatcgcc gccacgggca gcagccacat ggtttcaatt
      601 aacatgccgg tttgtgcttc taccgcaatc ttcttgcgca ccaggccgta gaaggcaaaa
      661 ctaaatgcca gccccagcgc gataataggt agcgaaccaa aagtccacag ctggactaac
      721 acgccacata tcgccagaat caccgccagc cattgcatcc ggcggaatcg ctcgccgagg
      781 aaaatcatcc ccagcacaat gttcaccagc gggttaataa agtaaccaag gctcgcttcc
      841 agcatatggt gattgttcac cgcccagata aacagtagcc agttgccacc aatcagcacg
      901 gcagagactg ccagcataaa aattttctgt ggcgtctgaa tcagcgtttt taaataggac
      961 cactggcggc aaatgctcat cagcaccacc ataaagaaaa acgaccagat cacgcgatgc
     1021 gtcaggatct catcggtggg cacgtagtaa atcaacttga agtacgctgg ggctataccc
     1081 caaataaaat aagcggcaag agcgagtaat acgccctgcc gcgtttgttt tgcatccatc
     1141 gggaatactc atttttaatt ggtaacagca gtttacctgc ttttatgtct tcaacctacc
     1201 atataggtgg cggtggcact ggcaatataa agctgttctt cattgtgtaa ttcaacgcgg
     1261 gcgacggcga ctttattgcc tgcacgcaac aggctactgg tagcagtaaa acgctcgcct
     1321 ctgcctgggc gcagataatc gacgcgaaga tcaatggtcc ccatccgcga tagccgctgg
     1381 cgtagttcat cttcactgat ggtttcgtgg cgggttaagg tacttcctac gcacaccaga
     1441 ccggcggcga catccagcgc cgacgcaatg accccgccgt gcaaaatgct ttgcgcccag
     1501 ttgcccacca tcattggctg atttttaaag gccagctgtg cgaactcttt ttcgtaacgc
     1561 tccagttcca tccccaatgc gcggttaaat ggcatgtgat aaacaaacat ctcacccact
     1621 aatttcaggg cttgttcagc ggtcagtacg gcagacatat catccttaca cttcattggt
     1681 taatgaaatg ttgattttat gattctttgt tgttggtttc tactttagga agggataact
     1741 aacggcaatg gagttaagta tgtaaaatac cccgcagaaa aacattcacc ttatcaataa
     1801 ttcgttacgg agaacacgac cgatgcggac tctgcagggc tggttgttgc cggtgtttat
     1861 gttgcctatg gcagtatatg cacaagaggc aacggtgaaa gaggtgcatg acgcgccagc
     1921 ggtgcgtggc agtattatcg ccaatatgct gcaggagcat gacaatccgt tcacgctcta
     1981 tccttatgac accaactacc tcatctacac ccaaaccagc gatctgaaca aagaagcgat
     2041 tgccagttac gactgggcgg aaaatgcgcg taaggatgaa gtaaagtttc agttgagcct
     2101 ggcatttccg ctgtggcgtg ggattttagg cccgaactcg gtgttgggtg cgtcttatac
     2161 gcaaaaatcc tggtggcaac tgtccaatag cgaagagtct tcaccgtttc gtgaaaccaa
     2221 ctacgaaccg caattgttcc tcggttttgc caccgattac cgttttgcag gttggacgct
     2281 gcgcgatgtg gagatggggt ataaccacga ctctaacggg cgttcagacc cgacctcccg
     2341 cagctggaac cgcctttata ctcgcctgat ggcagaaaac ggtaactggc tggtagaagt
     2401 gaagccgtgg tatgtggtgg gtaatactga cgataacccg gatatcacca aatatatggg
     2461 ttactaccag cttaaaatcg gctatcacct cggtgatgcg gtgctcagtg cgaaaggaca
     2521 gtacaactgg aacaccggct acggcggcgc ggagttaggc ttaagttacc cgatcaccaa
     2581 acatgtgcgc ctttatactc aggtttacag cggctatggc gaatcgctca tcgactataa
     2641 cttcaaccag acccgtgtcg gtgtgggggt tatgctaaac gatttgtttt gatgaacggt
     2701 tgagtggttg gcaaatctgg aatccagcat ccaggattac cctctcagag actaaaagca
     2761 ttgcagtttc tcgcgcaggc gctgaaaata gcgcctgttt ttatttcagg caatcggggt
     2821 gaatgtggcg caggcggaag tgttgaatct ggagtccgga gctaaacagg ttttacaaga
     2881 aacctttggc taccaacagt ttcgccccgg ccaggaagaa attatcgaca ctgtgctttc
     2941 cggtcgcgat tgcctggtcg tcatgcccac cggtggcgga aaatcccttt gctatcaaat
     3001 tcctgcctta ttgctaaacg gccttaccgt ggttgtttca ccgctgattt cgttgatgaa
     3061 agatcaggtg gatcaactgc aagccaacgg cgtggcggcg gcgtgcctta actcgacgca
     3121 aacccgcgaa cagcaacttg aagtgatgac aggctgccgc accgggcaaa ttcgtctgct
     3181 ttatatcgcc ccggaacgcc tgatgctgga taactttctt gagcatctgg cgcactggaa
     3241 tccggtgtta ttagccgttg atgaagcgca ctgtatctcc caatggggcc acgatttccg
     3301 cccggaatat gccgcgctcg gtcagttgcg ccagcggttc ccgacgctgc cgtttatggc
     3361 gctgaccgcc acagccgacg acaccacgcg ccaggatatc gtgcgcctgc tggggctgaa
     3421 cgatccgctg attcaaatca gcagttttga ccgtccgaat attcgctaca tgctgatgga
     3481 gaaattcaaa ccgctcgatc agttgatgcg ctacgtgcag gaacagcgcg gtaagtccgg
     3541 cattatctac tgcaacagcc gggcgaaagt agaagacacc gctgcgcgcc tgcaaagcaa
     3601 gggtattagc gcggcggcct atcatgccgg gctggaaaat aatgtccgcg ccgacgtgca
     3661 ggagaaattc cagcgcgatg acctgcaaat tgtggtggcg acggtggcgt tcggcatggg
     3721 catcaataaa ccaaacgttc gcttcgtggt ccactttgat attccgcgca atatcgaatc
     3781 ctattatcag gaaaccggtc gcgccgggcg tgatggcctg cctgcggaag cgatgttgtt
     3841 ttacgatcca gctgatatgg cgtggctgcg ccgttgtctg gaagagaagc cgcaggggca
     3901 gttgcaggat atcgagcgcc acaaactcaa tgcgatgggc gcgtttgccg aagcgcaaac
     3961 ttgccgtcgt ctggtattgc tgaactattt tggcgaaggg cgtcaggagc cgtgcgggaa
     4021 ctgcgatatc tgcctcgatc cgccgaaaca gtacgacggt tcaaccgatg ctcagattgc
     4081 cctttccacc attggtcgtg tgaatcagcg gtttgggatg ggttatgtgg tggaagtgat
     4141 tcgtggtgct aataaccagc gtatccgcga ctatggtcat gacaaactga aagtctatgg
     4201 catgggccgt gataaaagcc atgaacattg ggtgagcgtg atccgccagc tgattcacct
     4261 cggcctggtg acgcaaaata ttgcccagca ttctgcccta caactgacag aggccgcgcg
     4321 cccggtgctg cgcggcgaat cctctttaca acttgccgtg ccgcgtatcg tggcgctcaa
     4381 accgaaagcg atgcagaaat cgttcggcgg caactatgat cgcaaactgt tcgccaaatt
     4441 acgcaaactg cgtaaatcga ttgccgatga aagtaatgtc ccgccgtacg tggtgtttaa
     4501 cgacgcaacc ttgattgaga tggctgaaca gatgccgatc accgccagcg aaatgctcag
     4561 cgttaacggc gttgggatgc gcaagctgga acgctttggt aaaccgttta tggcgctgat
     4621 tcgtgcgcat gtcgatggcg acgacgaaga gtagtcagca gcataaaaaa gtgccaggat
     4681 gaagactccg taaacgtttc ccccgcgagt caaatgtatg ttgatgttat ttctcaccgt
     4741 cgccatggtg cacattgtgg cgcttatgag tcccggtccc gatttctttt ttgtctctca
     4801 gaccgctgtc agtcgttccc gtaaagaagc gatgatgggc gtgctgggta ttacctgcgg
     4861 cgttatggtt tgggcaggga ttgcgttgct tggcctgcat ttgattatcg aaaaaatggc
     4921 ctggctgcat acgctgatta tggttggcgg tggcctgtat ctctgctgga tgggttacca
     4981 gatgctgcgc ggcgcgctga aaaaagaggc ggtttctgca cctgcgccac aggtcgagct
     5041 ggcgaaaagt gggcgcagtt tcctgaaagg tttactgacc aatctcgcta atccgaaagc
     5101 gattatctac tttggctcgg tgttctcatt gtttgtcggt gataacgttg gcactaccgc
     5161 gcgctggggc attttcgcgt tgattattgt cgaaacgctg gcgtggttta ctgtggtggc
     5221 cagcctgttt gccctgccgc aaatgcgccg tggttatcaa cgtctggcga agtggattga
     5281 tggttttgcc ggggcgttat ttgccggatt tggcattcat ttgattattt cgcggtaatg
     5341 ccagacgcgt cttcagagta agtcggataa ggcgtttacg ccgcatccga cattattttt
     5401 cacgcatgcc tcgccgatgc taacagcgct cccaccagca taaacaacga gccgaaaatc
     5461 ttattcagcg ccttcatctg ctttggtcct ttaatccata gagcaatccg ttgagcaagg
     5521 gtggcgtaac cgatcatcac aataatatcg accacaatag tggtgacacc gagcacgata
     5581 tactgcatca gttgcggctg ttgcggcatg atgaattgcg gaaatagcgc cgccagaaac
     5641 acaatacttt tgggattggt gagattcaca aaaactgcgc gctggaacaa atgtcgacgc
     5701 gattgagtag aggccagcga tttaagatca attgcgccag cggcgcgcca ttgctgtatt
     5761 cccagccaaa tcaagtaagc cgcgcctgcc cacttcaaca cttcaaacgc aatcactgag
     5821 cgggaaaata gcgtccccaa ccccacgcca accagcacaa tatgaatcgc cagtccggtc
     5881 tgaagcccgg caatagacgc caccgcgccg cgataaccgt ggttgagcga ggtggtcata
     5941 gtgttgattg caccagagcc tggcgacagg cttaaaatga ttgatgtcag taggtaggca
     6001 aaccaccatt ctaaggtcat gatgaactcc cggtgtgtct atttttgtgc cacaatacgc
     6061 tactgtcgca gcgttgtgtc aggcacgcta aaaaaaacga ttttacgtgg tttaagaggc
     6121 agattacccg atgtttcagc agcaaaaaga ctgggaaaca agagaaaacg cgtttgctgc
     6181 ttttaccatg ggaccgctga ctgatttctg gcgtcagcgt gatgaagcag agtttactgg
     6241 tgtggatgac attccggtgc gctttgtccg ttttcgcgca cagcaccatg accgggtggt
     6301 agtcatctgc ccggggcgta ttgagagtta cgtaaaatat gcggaactgg cctacgattt
     6361 gttccatttg gggtttgatg tcttaatcat cgaccatcgc gggcagggac gttccggtcg
     6421 cctgttagcc gatccgcatc tcgggcatgt taatcgcttt aatgattatg ttgatgatct
     6481 ggcggcattc tggcaacagg aggtccagcc cggtccgtgg cgtaaacgct atatactggc
     6541 acattcgatg ggcggtgcga tctccacatt atttctgcaa cgccatccag gtgtatgtga
     6601 cgccattgcg ctaactgcgc caatgtttgg gatcgtgatt cgtatgccgt catttatggc
     6661 acggcagatc ctcaactggg ccgaagcgca tccacgtttc cgtgatggct atgcaatagg
     6721 caccgggcgc tggcgcgcgt tgccgtttgc tatcaacgta ctgacccaca gcagacagcg
     6781 atatcgacgt aacttacgct tctatgctga tgacccaacg attcgcgtcg gtgggccgac
     6841 ctaccattgg gtacgcgaaa gtattctggc tggcgaacag gtgttagccg gtgcgggtga
     6901 tgacgccacg ccaacgcttc tcttgcaggc tgaagaggaa cgcgtggtgg ataaccgcat
     6961 tcatgaccgt ttttgtgaac tccgcaccgc cgcgggccat cctgtcgaag ggggacgacc
     7021 gctggtaatt aaaggtgcgt accatgagat cctttttgaa aaggacgcaa tgcgctcagt
     7081 tgcgctccac gccatcgttg attttttcaa caggcataac tcacccagcg gaaaccgctc
     7141 tacagaggtt taaatttctt atgtaccagg ttgttgcgtc tgatttagat ggcacgttac
     7201 tttctcccga ccatacatta tccccttacg ccaaagaaac cctgaagctg ctcaccgcgc
     7261 gcggcatcaa ctttgtgttt gcgaccggtc gtcaccacgt tgatgtgggg caaattcgcg
     7321 ataatctgga gattaagtct tacatgatta cctccaatgg tgcgcgcgtt cacgatctgg
     7381 atggtaatct gatttttgct cataacctgg atcgcgacat tgccagcgat ctgtttggcg
     7441 tagtcaacga caatccggac atcattacta acgtttatcg cgacgacgaa tggtttatga
     7501 atcgccatcg cccggaagag atgcgctttt ttaaagaagc ggtgttcaaa tatgcgctgt
     7561 atgagcctgg attactggag ccggaaggcg tcagcaaagt gttcttcacc tgcgattccc
     7621 atgaacaact gctgccgctg gagcaggcga ttaacgctcg ttggggcgat cgcgtcaacg
     7681 tcagtttctc taccttaacc tgtctggaag tgatggcggg cggcgtttca aaaggccatg
     7741 cgctggaagc ggtggcgaag aaactgggct acagcctgaa ggattgtatt gcgtttggtg
     7801 acgggatgaa cgatgccgaa atgctgtcga tggcggggaa aggctgcatt atgggcagtg
     7861 cgcaccagcg tctgaaagac cttcatcccg agctggaagt gattggtact aatgccgacg
     7921 acgcggtgcc gcattatctg cgtaaactct atttatcgta atcgttcttt atttggtcag
     7981 ttgtcaacct gatacttcgc tacaatggat actcgttaat caaagagttt tccattgtgg
     8041 cgctacttat catcaccacg attctgtggg ccttctcctt tagcttttat ggcgagtacc
     8101 ttgcggggca cgtcgatagc tattttgcgg tgctggtgcg cgttggcctg gcggcactcg
     8161 tttttctgcc gtttctgcgt acccgtggca atagcctgaa aacagtcggc ctgtatatgc
     8221 tggtgggcgc gatgcagctc ggcgtgatgt atatgctgag tttccgcgct tatctctacc
     8281 tgacggtttc cgaactgctg ctgttcaccg tactgacgcc gctctacatc acgctgattt
     8341 atgacatcat gagtaagcgc cgtctgcgct ggggatatgc ttttagtgcc ttgttggcgg
     8401 tgattggtgc cgggattatt cgctatgatc aggtcaccga ccatttctgg actggcttgt
     8461 tgctggtgca actctccaat atcacttttg ccatcggcat ggtgggttac aaacgcctga
     8521 tggaaactcg cccgatgccg cagcataacg cctttgcgtg gttctatctt ggcgcgttcc
     8581 tggtggcggt aattgcatgg ttcttgctgg gaaatgcgca gaaaatgccg caaaccacgt
     8641 tgcaatgggg cattctggtg tttcttggcg tggtggcttc cgggattggc tactttatgt
     8701 ggaactacgg cgcaacccaa gtagacgccg gaacgctggg cattatgaat aatatgcacg
     8761 ttccggcagg gctgctggtt aacctggcta tctggcacca acagccgcac tggccaacgt
     8821 ttattacagg cgcgctggtg atcctggcct cactgtgggt gcatcgtaag tgggtcgctc
     8881 cgcgctcttc acaaacggca gatgatcgca ggcgtgattg cgcgctgagc gaataaacgc
     8941 ttccgtaact ggctgacgct gctcgccatc gcgcacggcg gcgtacagtc ggctccacaa
     9001 gccttcgccc agggttttgg tcaccaccag accctggcgc tcaaaactct ctactaccca
     9061 atgtggtagc gcggcaatac ccatccgcgc ggcaaccatc tgaatcaaca ataaggtgtt
     9121 atcgacgctt ttcagcgacg ggctaacgcc tgcgggctgg agaaaatgcc gccagacatc
     9181 cagtcggcta cgctgcaccg gataaatcaa cagcgtttcg ctggcgagat cttccggcgt
     9241 gattcgcgtt ttcgccgcca gtggatggtc aggtgctaac accagacgca cttcatagtc
     9301 gaacatcggc gaataatgca ggccactacg cggcagaata tcggaggtca tcaccagatc
     9361 cagctcgccc tgctgcaacg caggctgcgg gtcaaacgtt acgcccgatt taaaatccat
     9421 ctccacctgc ggccagttct tatggaaatt ttctaatgcg ggcgtcagcc actgaataca
     9481 gctatggcac tcaatggcaa tgcgcaggcg cgtctgttgc ggttcattgc aggcttgcag
     9541 cgcctggcta atttgcggca gcacctggtt tgccagttgc aacaggattt ccccctgtgg
     9601 agtaaagcgt agcggctggc tcttacgcac aaatagccgg aagccaaggc gttgttccag
     9661 atcgctaaac tggtgagaca gggcggattg cgtttgatgc aacgtcgccg cagcggctgc
     9721 gagagagccg cagttccgca acgcttgtag cgttttcagg tgttttactt cgatcatgaa
     9781 agtccttcac ttcggcatga ataatttgcg cttgaggaat atacagtaac cgccaattat
     9841 ggatgtgtaa acatctggac ggctaaaatc cttcgtcttt taaatttatg gtgcgttggc
     9901 tgcgtttctc caccccggtc acttacttca gtaagctccc ggggatgaat aaacttgccg
     9961 ccttccctaa attcaaaatc cataggattt acatataatt agaggaagaa aaatgacaat
    10021 attgaatcac accctcggtt tccctcgcgt tggcctgcgt cgcgagctga aaaaagcgca
    10081 agagagttat tgggcgggga actccacgcg tgaagaactg ctggcggtag ggcgtgaatt
    10141 gcgtgctcgt cactgggatc aacaaaagca agcgggtatc gacctgctgc cggtgggcga
    10201 ttttgcctgg tacgatcatg tactgaccac cagtctgctg ctgggtaacg ttccggcgcg
    10261 tcatcagaac aaagatggtt cggtagatat cgacaccctg ttccgtattg gtcgtggacg
    10321 tgcgccgact ggcgaacctg cggcggcagc ggaaatgacc aaatggttta acaccaacta
    10381 tcactacatg gtgccggagt tcgttaaagg ccaacagttc aaactgacct ggacgcagct
    10441 gctggacgaa gtggacgagg cgctggcgct gggccacaag gtgaaacctg tgctgctggg
    10501 gccgattacc tggctgtggc tggggaaagt gaaaggtgaa cagtttgacc gcctgagcct
    10561 gctgaacgac attctgccgg tttatcagca agtgctggca gaactggcga aacgtggcat
    10621 cgagtgggta cagattgatg aacccgcgct ggtactggaa ctaccacagg cgtggctgga
    10681 cgcatacaaa cccgcttacg acgcgctcca gggacaggtg aaactgctgc tgaccaccta
    10741 ttttgaaggc gtaacgccaa atctcgacac gattactgcg ctgcctgttc aggggctgca
    10801 tgtggacctt gtacatggta aagatgacgt tgttgaactg cacaagcgcc tgccttctga
    10861 ctggctgctg tctgcgggtc tgatcaatgg tcgtaacgtc tggcgcgccg atcttactga
    10921 gaaatatgtg caaattaagg acattgtcgg caaacgtgat ttgtgggtgg catcttcctg
    10981 ctcgttgctg cacagcccca tcgacctgag cgtggaaacg cgtcttgatg cagaagtgaa
    11041 aagctggttt gccttcgccc tacaaaaatg ccatgaactg gcactgctgc gcgatgcgct
    11101 gaacagtggt gacacggcag ctctggcaga gtggagcgcc ccaattcagg cgcgtcgtca
    11161 ctctactcgt gtacataatc cggcggtaga aaagcgtctg gcggcgatca ccgcccagga
    11221 cagccagcgt gcgaatgtct atgaagtgcg tgctgaagcc cagcgtgcgc gttttaaact
    11281 gccagcgtgg ccgaccacca cgattggttc cttcccgcaa accacggaaa ttcgtaccct
    11341 gcgtctggat ttcaaaaagg gcaatctcga cgccaataac taccgcacgg gcattgcgga
    11401 acatatcagg caggccattg ttgaacagga acgtttggga ctggatgtgc tggtacatgg
    11461 cgaggccgag cgtaatgaca tggtggaata ctttggcgag cacctcgacg ggtttgtctt
    11521 tacgcaaaac ggttgggtac agagctacgg ttcccgctgc gtgaagccac cgattgttat
    11581 tggtgacgtt agtcgcccgg caccgattac cgtggagtgg gcgaagtatg cgcaatctat
    11641 gactgataaa ccggtgaaag ggatgctgac tggcccggtg accattctct gctggtcgtt
    11701 cccgcgtgaa gatgtcagcc gtgaaaccat cgccaaacaa attgcgctgg cgctgcgtga
    11761 tgaagtggcg gacctggaag ccgcaggaat tggcatcatc cagattgatg aaccggcgct
    11821 gcgcgaaggt ttaccgctgc gtcgtagcga ctgggatgca tatctccagt ggggcgtgga
    11881 ggccttccgt atcaacgccg ccgtggcgaa agatgacaca caaatccaca ctcacatgtg
    11941 ttattgcgag ttcaacgaca tcatggattc gattgcggca ctggacgcag acgtcatcac
    12001 catcgaaacc tcgcgttctg acatggagtt gctggagtcg ttcgaagagt ttgattatcc
    12061 gaatgaaatc ggtccaggcg tctatgacat tcactcgcca aacgtaccga gcgtggaatg
    12121 gattgaagcc ttgctgaaga aagcggcaaa acgcattccg gcagagcgcc tgtgggtcaa
    12181 cccggactgt ggcctgaaaa cgcgcggctg gccagaaacc cgcgcggcac tggcgaacat
    12241 ggtgcaggcg gcgcagaact tgcgtcgggg gtaaaatcca aaccgggtgg taataccacc
    12301 cggtcttttc tcattacagc gacttcttcc caccatactg cttaaaccat tccagcatac
    12361 gctgccagcc atcttctgca gatgcggcat gatagctcgg gcgataatca gcgttgaatg
    12421 catgcccggc gtccgggtac acgataatct ctgctttcgc attagcagcc cgcagcgcct
    12481 ggcgcatggt ttcaacgctc tcctgcggaa tactgttatc cagaccgcca tataagccga
    12541 gaaccggcgc gttaagatcg gttgcgatat caacaggttg tttcggtgaa ttcagcgact
    12601 tatcgcccgt cagtttgccg taccacgcca ctgcggcttt tagctgtgga ttatgcgcgg
    12661 catacagcca agtgatacgt ccaccccagc agaatccggt gatcattaaa cgatgaacat
    12721 cgccgccgtt gcgggaagcc caactggcga catgatcgag atcggccagc acctgcgagt
    12781 caggcacttt tgctaccaga ccgctaagca acgtggggat atcggcaaaa tcattcggat
    12841 cgccttcgcg gaagtaaagt tcaggtgcga tagccagata cccctccagc gccagacggc
    12901 gacaaatgtc gcggatatgt tcatgcacgc caaaaatttc ctgcactaca atgaccactg
    12961 gcagtgggcc atcgctttgc tttggtctgg catggtaagc aggcatgtta tccccttgtg
    13021 aagggataga ggtgaagccc gccacaatcg cgtcgtccgg ggtctgaacg atggtcgaag
    13081 cgagaggcga tgcagcaggt gcaaatccag attgttgtgt tgttgccatg gtattctccg
    13141 tacccttata aaaatgttgc gcaatgttaa ctatagtcag catgcaacaa atcacattgc
    13201 ctgaatcggc tcatctttta tgcagtcctg cagaatgaag agtgatttat gtgatttgca
    13261 tcacctttgg tgggtaaatt tatgcaacgc atttgcgtca tggtgatgag tatcacgaaa
    13321 aaatgttaaa cccttcggta aagtgtcttt ttgcttcttc tgactaaacc gattcacaga
    13381 ggagttgtat atgtccaagt ctgatgtttt tcatctcggc ctcactaaaa acgatttaca
    13441 aggggctacg cttgccatcg tccctggcga cccggatcgt gtggaaaaga tcgccgcgct
    13501 gatggataag ccggttaagc tggcatctca ccgcgaattc actacctggc gtgcagagct
    13561 ggatggtaaa cctgttatcg tctgctctac cggtatcggc ggcccgtcta cctctattgc
    13621 tgttgaagag ctggcacagc tgggcattcg caccttcctg cgtatcggta caacgggcgc
    13681 tattcagccg catattaatg tgggtgatgt cctggttacc acggcgtctg tccgtctgga
    13741 tggcgcgagc ctgcacttcg caccgctgga attcccggct gtcgctgatt tcgaatgtac
    13801 gactgcgctg gttgaagctg cgaaatccat tggcgcgaca actcacgttg gcgtgacagc
    13861 ttcttctgat accttctacc caggtcagga acgttacgat acttactctg gtcgcgtagt
    13921 tcgtcacttt aaaggttcta tggaagagtg gcaggcgatg ggcgtaatga actatgaaat
    13981 ggaatctgca accctgctga ccatgtgtgc aagtcagggc ctgcgtgccg gtatggtagc
    14041 gggtgttatc gttaaccgca cccagcaaga gatcccgaat gctgagacga tgaaacaaac
    14101 tgaaagccaa gcggtgaaaa tcgtggtgga agcggcgcgt cgtctgctgt aattctcttc
    14161 tcctgtctga aggccgacgc gttcggcctt ttgtattttt gcgtagcgcc tcgcaggaaa
    14221 tgcctttcca actggacgtt tgtacagcac aattctattt tgtgcgggta agttgttgcg
    14281 tcaggaggcg ttgtggattt ctcaatcatg gtttacgcag ttattgcgtt ggtgggtgtg
    14341 gcaattggct ggctgtttgc cagttatcaa catgcgcagc aaaaagccga gcaattagct
    14401 gaacgtgaag agatggtcgc ggagttaagc gcggcaaaac aacaaattac ccaaagcgag
    14461 cactggcgtg cagagtgcga gttactcaat aacgaagtgc gcagcctgca aagtattaac
    14521 acctctctgg aggccgatct gcgtgaagta accacgcgga tggaagccgc acagcaacat
    14581 gctgacgata aaattcgcca gatgattaac agcgagcagc gcctcagtga gcagtttgaa
    14641 aacctcgcca accgtatttt tgagcacagc aatcgccggg ttgatgagca aaaccgtcag
    14701 agtctgaaca gcctgttgtc gccgctacgt gaacaactgg acggtttccg ccgtcaggtt
    14761 caggacagct tcggtaaaga agcacaagaa cgccataccc tgacccacga aattcgcaat
    14821 ctccagcaac tcaacgcgca aatggcccag gaagcgatca acctgacgcg cgcgctgaaa
    14881 ggcgacaata aaacccaggg caactggggc gaggtagtat tgacgcgggt gctggaggct
    14941 tccggtctgc gtgaagggta tgaatatgaa acccaggtca gcatcgaaaa tgacgcccgc
    15001 tcgcggatgc agccggatgt catcgtgcgg ctgccgcagg gaaaagatgt ggtgatcgac
    15061 gccaaaatga cgctggtcgc ctatgaacgc tattttaacg ccgaagacga ctacacccgc
    15121 gaaagcgcgc tacaggaaca tatcgcgtcg gtgcgtaacc atatccgttt gctgggacgc
    15181 aaagattatc aacagctgcc ggggctgcga actctggatt acgtgctgat gtttattccc
    15241 gttgaacccg cttttttact ggcgcttgac cgccagccgg agctgatcac cgaagcgttg
    15301 aaaaacaata tcatgctggt tagcccgact acgctgctgg tggcgctgcg cactatcgcc
    15361 aacctgtggc gttatgagca tcaaagccgc aacgcccagc aaatcgccga tcgtgccagc
    15421 aagctgtacg acaagatgcg tttgttcatc gatgacatgt ccgcgattgg tcaaagtctc
    15481 gacaaagcgc aggataacta tcggcaggca atgaaaaaac tctcttcagg gcgcggaaat
    15541 gtgctggcgc aggcagaagc gtttcgcggt ttaggagtag aaattaaacg cgagattaat
    15601 ccggatttgg ctgaacaggc ggtgagccag gatgaagagt atcgacttcg gtcggttccg
    15661 gagcagccga atgatgaagc ttatcaacgc gatgatgaat ataatcagca gtcgcgctag
    15721 cccattggga gtagttaagc cgggtagaaa tctagggcat cgacgcccaa tctgttacac
    15781 ttctggaaca attttttgat gagcaggcat tgagatggtg gataagtcac aagaaacgac
    15841 gcactttggt tttcagaccg tcgcgaagga acaaaaagcg gatatggtcg cccacgtttt
    15901 ccattccgtg gcatcaaaat acgatgtcat gaatgatttg atgtcatttg gtattcatcg
    15961 tttgtggaag cgattcacga ttgattgcag cggcgtacgc cgtgggcaga ccgtgctgga
    16021 tctggctggt ggcaccggcg acctgacagc gaaattctcc cgcctggtcg gagaaactgg
    16081 caaagtggtc cttgctgata tcaatgaatc catgctcaaa atgggccgcg agaagctgcg
    16141 taatatcggt gtgattggca acgttgagta tgttcaggcg aacgctgagg cgctgccgtt
    16201 cccggataac acctttgatt gcatcaccat ttcgtttggt ctgcgtaacg tcaccgacaa
    16261 agataaagca ctgcgttcaa tgtatcgcgt gctgaaaccc ggcggccgcc tgctggtgct
    16321 tgagttctcg aagccaatta tcgagccgct gagcaaagcc tatgatgcat actccttcca
    16381 tgtgctgccg cgtattggct cactggtcgc gaacgacgcc gacagctacc gttatctggc
    16441 agaatccatc cgtatgcatc ccgatcagga taccctgaaa gccatgatgc aggatgccgg
    16501 attcgaaagt gtcgactact acaatctgac ggcaggggtt gtggcgctgc atcgtggtta
    16561 taagttctga caggagaccg gaaatgcctt ttaaaccttt agtgacggca ggaattgaaa
    16621 gtctgctcaa caccttcctg tatcgctcac ccgcgctgaa aacggcccgc tcgcgtctgt
    16681 tgggcaaagt gctgcgcgtg gaggtaaaag gcttttcgac gtcattgatt ctggtgttca
    16741 gcgaacgcca ggttgatgta ctgggcgaat gggcaggcga tgctgactgc accgttatcg
    16801 cctacgccag tgtgttgccg aaacttcgcg atcgccagca gcttgccgcc ctgattcgca
    16861 gtggtgagct ggaagtgcag ggcgatattc aggtggtgca aaacttcgtt gcgctggcag
    16921 gtctggcaga gttcgaccct gcggaactgc tggcccctta taccggtgat atcgccgctg
    16981 aaggaatcag caaagccatg cgcggaggcg caaagttcct gcatcacggc attaagcgcc
    17041 agcaacgtta tgtggcggaa gccattactg aagagtggcg tatggcaccc ggtccgcttg
    17101 aagtggcctg gtttgcggaa gagacggctg ccgtcgagcg tgctgttgat gccctgatca
    17161 aacggctgga aaaactggag gctaaatgac gccaggtgaa gtacggcgcc tatatttcat
    17221 cattcgcact tttttaagct acggacttga tgaactgatc cccaaaatgc gtatcaccct
    17281 gccgctacgg ctatggcgat actcattatt ctggatgcca aatcggcata aagacaaact
    17341 tttaggtgag cgactacgac tggccctgca agaactgggg ccggtatgga tcaagttcgg
    17401 gcaaatgtta tcaacccgcc gcgatctttt tccgccgcat attgccgatc agctggcgtt
    17461 attgcaggac aaagttgctc cgtttgatgg caagctggcg aagcagcaga ttgaagctgc
    17521 aatgggtggc ttgccggtag aagcgtggtt tgacgatttt gaaatcaagc cactggcttc
    17581 tgcttctatc gcccaggttc ataccgcgcg attgaaatcg aatggtaaag aggtggtgat
    17641 taaagtcatc cgcccggata ttttgccggt cattaaagca gacctgaaac ttatctaccg
    17701 tctggctcgc tgggtgccgc gtttgctgcc ggatggtcgc cgtctacgcc caaccgaagt
    17761 ggtgcgcgag tacgaaaaga ccttgattga tgaactgaat ttgctgcggg aatctgccaa
    17821 cgccattcag cttcggcgaa attttgaaga cagcccgatg ctctacatcc cggaggttta
    17881 ccctgactat tgtagtgaag ggatgatggt gatggagcgt atttacggca ttccggtgtc
    17941 tgatgttgcg gcgctggaga aaaacggcac caacatgaaa ttgctggcgg aacgcggcgt
    18001 gcaggtgttc ttcactcagg tctttcgcga cagctttttc catgccgata tgcaccctgg
    18061 caacatcttc gtaagctatg aacacccgga aaacccgaaa tatatcggca ttgattgcgg
    18121 gattgttggc tcgctaaaca aagaagataa acgctatctg gcagaaaact ttattgcctt
    18181 ctttaatcgc gactatcgca aagtggcaga gctacacgtc gattctggct gggtgccacc
    18241 agataccaac gttgaagagt tcgaatttgc tattcgtacg gtctgtgaac ctatctttga
    18301 gaaaccgctg gccgaaattt cgtttggaca tgtactgtta aatctgttta atacggcgcg
    18361 tcgcttcaat atggaagtgc agccgcaact ggtgttactc cagaaaaccc tgctctacgt
    18421 cgaaggggta ggacgccagc tttatccgca actcgattta tggaaaacgg cgaagccttt
    18481 cctggagtcg tggattaaag atcaggtcgg tattcctgcg ctggtgagag catttaaaga
    18541 aaaagcgccg ttctgggtcg aaaaaatgcc agaactgcct gaattggttt acgacagttt
    18601 gcgccagggc aagtatttac agcacagtgt tgataagatt gcccgcgagc ttcagtcaaa
    18661 tcatgtacgt cagggacaat cgcgttattt tctcggaatt ggcgctacgt tagtattaag
    18721 tggcacattc ttgttggtca gccgacctga atgggggctg atgcccggct ggttaatggc
    18781 aggtggtctg atcgcctggt ttgtcggttg gcgcaaaaca cgctgatttt ttcatcgctc
    18841 aaggcgggcc gtgtaacgta taatgcggct ttgtttaatc atcatctacc acagaggaac
    18901 atgtatgggt ggtatcagta tttggcagtt attgattatt gccgtcatcg ttgtactgct
    18961 ttttggcacc aaaaagctcg gctccatcgg ttccgatctt ggtgcgtcga tcaaaggctt
    19021 taaaaaagca atgagcgatg atgaaccaaa gcaggataaa accagtcagg atgctgattt
    19081 tactgcgaaa actatcgccg ataagcaggc ggatacgaat caggaacagg ctaaaacaga
    19141 agacgcgaag cgccacgata aagagcaggt gtaatccgtg tttgatatcg gttttagcga
    19201 actgctattg gtgttcatca tcggcctcgt cgttctgggg ccgcaacgac tgcctgtggc
    19261 ggtaaaaacg gtagcgggct ggattcgcgc gttgcgttca ctggcgacaa cggtgcagaa
    19321 cgaactgacc caggagttaa aactccagga gtttcaggac agtctgaaaa aggttgaaaa
    19381 ggcgagcctc actaacctga cgcccgaact gaaagcgtcg atggatgaac tacgccaggc
    19441 cgcggagtcg atgaagcgtt cctacgttgc aaacgatcct gaaaaggcga gcgatgaagc
    19501 gcacaccatc cataacccgg tggtgaaaga taatgaagct gcgcatgagg gtgtaacgcc
    19561 tgccgctgca caaacgcagg ccagttcgcc ggaacagaag ccagaaacca cgccagagcc
    19621 ggtggtaaaa cctgctgcgg acgctgaacc gaaaaccgct gcaccttccc cttcgtcgag
    19681 tgataaaccg taaacatgtc tgtagaagat actcaaccgc ttatcacgca tctgattgag
    19741 ctgcgtaagc gtctgctgaa ctgcattatc gcggtgatcg tgatattcct gtgtctggtc
    19801 tatttcgcca atgacatcta tcacctggta tccgcgccat tgatcaagca gttgccgcaa
    19861 ggttcaacga tgatcgccac cgacgtggcc tcgccgttct ttacgccgat caagctgacc
    19921 tttatggtgt cgctgattct gtcagcgccg gtgattctct atcaggtgtg ggcatttatc
    19981 gccccagcgc tgtataagca tgaacgtcgc ctggtggtgc cgctgctggt ttccagctct
    20041 ctgctatttt atatcggcat ggcgttcgcc tactttgtgg tctttccgct ggcatttggc
    20101 ttccttgcca ataccgcgcc ggaaggggtg caggtatcca ccgacatcgc cagctattta
    20161 agcttcgtta tggcgctgtt tatggcgttt ggtgtctcct ttgaagtgcc ggtggcaatt
    20221 gtgctgctgt gctggatggg gattacctcg ccagaagact tacgcaaaaa acgcccgtat
    20281 gtgctggttg gtgcattcgt tgtcgggatg ttgctgacgc cgccggatgt cttctcgcaa
    20341 acgctgttgg cgatcccgat gtactgtctg tttgaaatcg gtgtcttctt ctcacgcttt
    20401 tacgttggta aagggcgaaa tcgggaagag gaaaacgacg ctgaagcaga aagcgaaaaa
    20461 actgaagaat aaattcaacc gcccgtcagg gcggttgtca tatggagtac aggatgtttg
    20521 atatcggcgt taatttgacc agttcgcaat ttgcgaaaga ccgtgatgat gttgtagcgc
    20581 gcgcttttga cgcgggagtt aatgggctac tcatcaccgg cactaacctg cgtgaaagcc
    20641 agcaggcgca aaagctggcg cgtcagtatt cgtcctgttg gtcaacggcg ggcgtacatc
    20701 ctcacgacag cagccagtgg caagctgcga ctgaagaagc gattattgag ctggccgcgc
    20761 agccagaagt ggtggcgatt ggtgaatgtg gtctcgactt taaccgcaac ttttcgacgc
    20821 cggaagagca ggaacgcgct tttgttgccc agctacgcat tgccgcagat ttaaacatgc
    20881 cggtatttat gcactgtcgc gatgcccacg agcggtttat gacattactg gagccgtggc
    20941 tggataaact gcctggtgcg gttcttcatt gctttaccgg cacacgcgaa gagatgcagg
    21001 cgtgcgtggc gcatggaatt tatatcggca ttaccggttg ggtttgcgat gaacgacgcg
    21061 gactggagct gcgggaactt ttgccgttga ttccggcgga aaaattactg atcgaaactg
    21121 atgcgccgta tctgctccct cgcgatctca cgccaaagcc atcatcccgg cgcaacgagc
    21181 cagcccatct gccccatatt ttgcaacgta ttgcgcactg gcgtggagaa gatgccgcat
    21241 ggctggctgc caccacggat gctaatgtca aaacactgtt tgggattgcg ttttagagtt
    21301 tgcggaactc ggtgttcttc acactgtgct taatctcttt attaataaga ttaagcaata
    21361 gcatggagcg agcctcacca tcgggttcgg tgaaaatggc ctgaaagcct tcgaacgcgc
    21421 cttcggtaat aatcacctta tcacccggat aaggggttgc cggatcgaca atgtctttcg
    21481 gtttatatac cgatagctga tgaatcaccg ccgatgggac tatcgctggc gacgcgccaa
    21541 agcgcacgaa gtggctgaca ccgcgggtcg cgttgatagt cgtggtatga atcacttctg
    21601 gatcaaattc cacaaacagg tagttgggga acaatggctc actgactgca gtacgtttgc
    21661 cacgcacgat tttttccagg gtgatcatcg gtgccaggca attcacagcc tgtctttcga
    21721 ggtgttcctg ggcacgttga agttgcccgc gcttgcagta cagtaaatac caggattgca
    21781 taatgactct tatccgttta atcggggcgc aaggatagca aaagctttac gctaagttaa
    21841 ttatattccc cggtttgcgt tataccgtca gagttcacgc taatttaaca aatttacagc
    21901 atcgcaaaga tgaacgccgt ataatgggcg cagattaaga ggctacaatg gacgccatga
    21961 aatataacga tttacgcgac ttcttgacgt tgcttgaaca gcagggtgag ctaaaacgta
    22021 tcacgctccc ggtggacccg catctggaaa tcactgaaat tgctgaccgc acgctgcgtg
    22081 ctggtgggcc tgcgctgttg tttgaaaacc ctaaagggta ctcaatgccg gtgctgtgca
    22141 acttgttcgg tacgccaaag cgcgtagcga tgggtatggg ccaggaagat gtttcagcac
    22201 tgcgtgaagt cggtaaatta ttagcatttc tgaaagaacc agagccgcca aaaggttttc
    22261 gcgatctgtt tgataagctg ccgcagttta agcaggtgtt aaacatgccg acaaagcgac
    22321 tgcgcggtgc accctgccaa caaaaaatcg tctctggcga tgacgtcgat ctcaaccgta
    22381 ttcccattat gacctgttgg ccggaagatg ccgcgccgct gattacatgg gggctaaccg
    22441 ttacacgtgg ccctcataaa gagcgacaga atctgggcat ttatcgccag caactgattg
    22501 gtaaaaacaa gctgattatg cgttggctgt cgcatcgcgg cggcgcgctg gattatcagg
    22561 agtggtgtgc ggcgcatcca ggtgaacgtt tcccgatctc tgtggcgttg ggcgctgatc
    22621 cggcaaccat tctcggtgca gtcacaccag taccagatac tttgtcggaa tacgcctttg
    22681 ccggattgct acgtggcacc aaaaccgaag tagtgaagtg tatttccaat gatctcgaag
    22741 tgcccgccag tgcggagatt gtgctggaag ggtatatcga acaaggcgaa atggcgccag
    22801 aaggaccgta tggtgaccac actggttact ataacgaagt cgatagtttc ccggtattta
    22861 ccgtgacgca tattacccag cgtgaagatg cgatttacca ttccacctat accgggcgtc
    22921 cgccagatga acccgcggta ctgggagtgg cgttgaacga agtatttgtt cccattctgc
    22981 aaaagcagtt cccggaaatt gtcgattttt acctgccgcc ggaaggctgc tcttatcgcc
    23041 tggcggtagt gacaatcaaa aaacagtacg ccggacacgc gaagcgcgtc atgatgggcg
    23101 tctggtcgtt cttacgccag tttatgtaca ctaaatttgt gatcgtttgc gatgatgacg
    23161 ttaacgcacg cgactggaac gatgtgattt gggcgattac cacccgtatg gacccagcgc
    23221 gggatactgt tctggtagaa aatacgccta ttgattatct ggattttgcc tcgcctgtct
    23281 ccgggctggg ttcaaaaatg gggctggatg ccacgaataa atggccgggg gaaacccagc
    23341 gtgaatgggg acgtcccatc aaaaaagatc cagatgttgt cgcacatatt gacgccatct
    23401 gggatgaact ggctattttt aacaacggta aaagcgcctg atgcgcgttt gttttgccct
    23461 atttatcgat ccgacagaga aagcgcatga caaccttaag ctgtaaagtg acctcggtag
    23521 aagctatcac ggataccgta tatcgtgtcc gcatcgtgcc agacgcggcc ttttcttttc
    23581 gtgctggtca gtatttgatg gtagtgatgg atgagcgcga caaacgtccg ttctcaatgg
    23641 cctcgacgcc ggatgaaaaa gggtttatcg agctgcatat tggcgcttct gaaatcaacc
    23701 tttacgcgaa agcagtcatg gaccgcatcc tcaaagatca tcaaatcgtg gtcgatattc
    23761 cccacggaga agcgtggctg cgcgatgatg aagagcgtcc gatgattttg attgcgggcg
    23821 gcaccgggtt ctcttatgcc cgctcgattt tgctgacagc gttggcgcgt aacccaaacc
    23881 gtgatatcac catttactgg ggcgggcgtg aagagcagca tctgtatgat ctctgcgagc
    23941 ttgaggcgct ttcgttgaag catcctggtc tgcaagtggt gccggtggtt gaacaaccgg
    24001 aagcgggctg gcgtgggcgt actggcaccg tgttaacggc ggtattgcag gatcacggta
    24061 cgctggcaga gcatgatatc tatattgccg gacgttttga gatggcgaaa attgcccgtg
    24121 acctgttttg cagtgagcgt aatgcgcggg aagatcgcct gtttggcgat gcgtttgcat
    24181 ttatctgaga tataaaaaaa cccgcccctg acaggcggga agaacggcaa ctaaaacttt
    24241 attcagtggc atttagatct atgtcgcatc cggcattcgg cacacgatgc ctgatgcgac
    24301 gctggcgcgt cttatcaggc ctacattggt gccggatcgg tagaccggat aaggcgttca
    24361 cgccgcatcc ggcaagtggt taaacccgct caaacaccgt cgcaataccc tgacccagac
    24421 cgatacacat cgtcgccaga ccaaactgaa cgtctttgtg ttccatcaga ttcagcagcg
    24481 tggtgctgat acgcgcaccg gaacaaccca gcggatgacc cagcgcgatc gcgccaccgt
    24541 tgaggttgat cttctcgtca atctgctcaa ttagtcccag atctttaata catggcagga
    24601 tctgcgcggc aaaggcttca ttcatttcaa acacgccgat atcactgaca gaaagccccg
    24661 cttttttcag cgccagcttc gaggccggaa ccgggccgta acccatgatc gatgggtcac
    24721 aaccaacgac cgccatcgaa cgcacacgag cgcgcggctt aagacctaat tcatgggcgc
    24781 ggctttcact catcaccagc atggcagctg cgccatcgga aagtgcagaa gatgtgcccg
    24841 ccgttaccgt accgtttact ggatcaaacg ccggacgcag cgtggcgagg gcttccacgg
    24901 tggtttccgg gcgaatcact tcgtcgtaat taaactgctt caggacgccg tcggcatcgt
    24961 gaccaccagt tgggatgatt tcatttttaa atgcgcccga ctgcgtggcg gcccaggcgc
    25021 gggcgtgtga ccgcgcggca aaggcatcct gcatttcacg gctgataccg tgcatacgcg
    25081 ccagcatttc tgccgttaag cccatcatgc ccgccgcttt ggcgacattg cggctcaggc
    25141 cagggtgaaa atcgacgccg tgactcatcg gcacatggcc catatgctcc acgccgccaa
    25201 ccagacatgc ctgcgcatcg ccggtcatga tcattcgtgc tgcgtcatgc agtgcctgca
    25261 tggatgaacc acacaagcga ttgacagtaa ccgccgggac agagtgcggc acttctgcca
    25321 gcagcgccgc gttacgggcg atattaaaac cctgctccag cgtctgctgc acacaccccc
    25381 agtaaatatc gtcgagggcc gccgcttcca gcgccgggtt acgcgccagc aggctacgca
    25441 ttaaatgagc ggagagatct tctgcacgca cgttacgaaa agcaccgccc ttcgaacggc
    25501 ccatcggggt gcgaattgca tcgacaatga caacctgttc cattgtgact ccttaagccg
    25561 ttttcaggtc gccaaccgga cgggctggct caaccggggg atagtacggt tcgttatgac
    25621 gcgctttatt acgcagacct tccggcactt cgtacagcgg gccgaggtgc tgatattgct
    25681 gtgccatatc gaggtatttt gcgctgccga gggtgtccag ccagcggaac gcgccgccgt
    25741 ggaacggagg gaagcccagg ccgtagacca gcgccatatc cgcttctgcc ggagtggcga
    25801 taatgccttc ttccagacag cgcaccactt cgttgaccat cgggatcatc atgcgggcga
    25861 taatctcttc ttcgctgaaa tcgcgcttcg gctggctcac ttctgccagc aggtcgtcaa
    25921 cagcggcgtc ttcttctttc ttcggcttac ctttgctgtc ttctttataa tgccagaaac
    25981 cgaggccgtt cttctgacca aagcggttgg catcaaacag cgcgtcgatg gcatcgcgat
    26041 aatctttctg catccgctgt gggaagcccg ctgccatgac tgcctgggcg tgatgcgcgg
    26101 tgtcaatgcc cacaacgtcc agcagatatg ccggacccat aggccagcca aactgttttt
    26161 ccatcacttt gtcgatcttg cggaaatccg cgccgtcgcg cagcaactgg ctgaaaccgg
    26221 cgaaatacgg gaacagcacg cggttgacga agaagccggg gcaatcgtta accacaatcg
    26281 gcgttttacc catcttgctc gcccaggcga cgactttcgc aatagtttcg tcggagcttt
    26341 tctcgccgcg aataatttcc accaacggca ttcggtggac cgggttaaag aagtgcatcc
    26401 cgcagaagtt ttccggacgt tccagcgcgt tggccagttc gctgatagga atggttgaag
    26461 tgttagacgc cagcacggta tccgggcgta ctttctgctc ggtttctgcc agcacggctt
    26521 ttttcacttt cgggttttca acaatcgctt ctaccacaac atccacgcgc tcaaatccgg
    26581 cgtagtcgag cgttgggtgg atcgtggaga tcacgccagc cagtttcaga ccatcgatct
    26641 tgccgcgctc aagctgcttg ttcagtagtt tcgcggcttc agtcatgccg agggctaacg
    26701 acttgtcgtt aatatctttc ataatcaccg gcacgccttt ccacgcagac tggtaagcga
    26761 tgccgccacc cataatgcct gcgcccagca cggcggcctg tttcggggtt tcaacgtctt
    26821 tggtgagttt cttcgctttg ccttttacat attgatcgtt aaggaaaatg ccgaccagtg
    26881 cgcgagcttc gttggtatgc gccagcggga caaagctttt gttttctagg tttaaggctt
    26941 cttcacgacc aaaacgagcc gcagcttcaa tggtttttac tgcggtgatg ggggccgggt
    27001 aatgttttcc tgccgtttgt gcgaccatcc ctttagcgat ggtgaagctc atggtggctt
    27061 caatcttgct cagttttagc ggttccagct tcggctgacg tttcgctttc cagtcgaggt
    27121 cgccgttaat ggcctggcgt aaaatcgcca tcgccccttc aatcagtttt tctgctttga
    27181 ctacgccatc caccagaccg attttcagcg cctgatccgc gccgacatct ttaccggcgg
    27241 caatgatttc cagcgcactg tcagcgccca gcatacgtgg catacgtaca gaaccgccaa
    27301 agcccggcat gatgcccagt ttggtttccg gcagaccgat gcgcagatcc ggcgtcgcca
    27361 gacggtaatc ggtcgccagc acgcattcgc agccaccgcc cagcgcatag ccattgacgg
    27421 cagcaatggt cggcaccggc agatcttcca ggcgattaaa cacgctattg gcaaagtgca
    27481 gccactgact taactgttct tcaggaacga ggaacaggga caaaaattcg gtgatatcag
    27541 caccgacgat aaaggctgct ttgttcgaac gcagcagcag cccttttaga tctgattgct
    27601 gttccagcac gccgatggcc tcgccgaggc tggcgacggt cgcagtgtcg agtttattaa
    27661 ctgaacctgg ggcatcaaat accagttcgg caatgccatc ttccagccag tcaaggtaca
    27721 gggtgtcgcc tttgtaaagc atgtcagtct cctgaatccg caaggtgatc tggtcgtacc
    27781 agatgcatcg aagtgtgtat tttgtgttaa aaatatgcaa ataaaagatt aaagaaatgc
    27841 cgatctgatc acgctcggca gaaatcacgc tctggatgaa cgatgtgcta agatgcggag
    27901 acttaaggtc aaaaaaacag aagggtaaaa aatggaatca ctggcctcgc tctataaaaa
    27961 tcatatagct acattgcagg aacgaactcg cgatgcgctg acgcgcttca agctggatgc
    28021 gttacttatt cactccggcg aactgttcaa cgtttttctc gacgatcatc cctatccgtt
    28081 taaagtgaac ccgcaattca aagcgtgggt gccggtaact caggtgccaa actgctggct
    28141 gctggtggat ggcgtgaata agccgaaact gtggttctat ctgccggttg attactggca
    28201 caacgtcgaa ccgctgccga cctccttctg gactgaagat gtagaagtga tcgcgctgcc
    28261 gaaagccgat ggcattggta gtctgttgcc tgctgcgcgc ggcaatatcg gttatatcgg
    28321 tccggtgccg gaacgtgcgc tgcaactggg tattgaggcc agcaatatca acccgaaagg
    28381 ggttatcgac tacctgcatt actaccgctc cttcaaaacc gagtacgagc tggcctgtat
    28441 gcgtgaagcg cagaaaatgg cggtcaacgg tcatcgcgcg gcagaagaag cgttccgttc
    28501 tggcatgagc gagttcgata tcaatattgc ctatctgact gcgaccggtc atcgtgatac
    28561 cgacgtacct tacagcaaca ttgtggcact caacgaacac gctgcggtgc tgcattacac
    28621 caaactggat catcaggcgt cggaagagat gcgcagcttc ctgctggatg ccggggccga
    28681 atataacggc tatgccgctg acctgacccg tacctggtcg gcaaaaagtg acaacgatta
    28741 cgcacagctg gtgaaagacg taaatgatga acaactggcg ctgatcgcga ccatgaaagc
    28801 tggcgttagc tatgtggatt accacatcca gttccatcag cgcatcgcca aattgctgcg
    28861 taaacatcaa atcatcaccg atatgagtga agaggcgatg gtcgaaaacg atcttaccgg
    28921 gccgtttatg ccgcatggta tcggccatcc gctgggcctg caggtgcatg acgtcgccgg
    28981 ttttatgcag gatgatagcg gtacgcacct cgcggcaccg gcaaaatatc cgtacctgcg
    29041 ctgcacccgt attctccagc cgggcatggt gttaaccatc gaaccgggta tctacttcat
    29101 tgaatcgctg ctggcaccgt ggcgtgaagg gcagttcagc aagcacttca actggcagaa
    29161 aattgaagca ctgaaaccgt tcggcggcat tcgtatcgaa gacaacgtgg tgatccacga
    29221 aaataacgtg gaaaacatga cccgggatct gaaactggcg tgatggaaag ctggttaatt
    29281 cctgcggcac cggtcacggt cgttgaagag atcaaaaaaa gccgcttcat tacgctgttg
    29341 gcgcataccg atggcgttga ggcggcgaaa gcgtttgttg aatcggtgcg ggcagaacac
    29401 cccgatgccc gccaccattg cgtggcgtgg gtggcgggtg cgccggatga ttctcaacag
    29461 ctgggtttct ctgacgacgg ggagccggcg ggaacggcag gtaaaccgat gctcgcccag
    29521 ctaatgggca gcggcgttgg ggaaattacc gctgtggtgg tgcgctacta cggcggcata
    29581 ttgcttggca ccggtgggtt agtgaaagcg tatggcggcg gcgtgaatca ggcgctgcgc
    29641 cagctaacga cccaacgcaa gacgccatta accgaatata ctttgcaatg tgaatatagt
    29701 cagttaaccg gcattgaagc gttgctgggg cagtgtgacg gcaaaattat caacagtgat
    29761 tatcaggcat tcgttctgct gcgggtggcg cttccggcgg cgaaagtggc tgaattttcc
    29821 gcgaagctgg cggattttag ccgtggttca ttgcaattgt tagcgattga agaataatcc
    29881 ccacttcgtt ttgcagaact aaggaagcgg cagagatgca ttttcgcgcc attacccgaa
    29941 tcgttggact actggtcatc ttattttcag ggaccatgat tatccctggg ctggtagcac
    30001 tcatctaccg ggatggagcg ggccgcgctt ttacccagac cttttttgtc gccctcgcca
    30061 ttggctctat gctgtggtgg ccgaaccgca aagagaaggg cgaactgaaa tcccgtgagg
    30121 ggtttctgat agtggtgctg ttctggaccg tgctgggtag tgtcggtgcg ctccctttta
    30181 tcttctcgga aagcccgaac ctcacgatta ccgatgcgtt ttttgaatct ttctctggcc
    30241 tgaccaccac cggggccact acgctggtgg ggctggattc gctccctcat gccatcctct
    30301 tttatcgcca gatgctgcaa tggtttggcg ggatggggat catcgtgtta gcggttgcga
    30361 tactgcctat cctcggcgtg ggtgggatgc agctctatcg cgcagaaatg cccggcccgc
    30421 tgaaagataa caaaatgcgc ccgcgaattg cggaaacggc gaaaaccctg tggttgattt
    30481 atgtcttgct gaccgtcgcc tgtgcgctgg cgttgtggtt tgccggaatg gatgcctttg
    30541 atgccatcgg ccatagcttt gcgactatcg ctattggcgg cttctcgaca catgatgcca
    30601 gtatcggtta tttcgatagc ccgactatta acactatcat tgctatcttc ctgctgatct
    30661 ccggctgtaa ctacggtctg cacttttcac tgttaagtgg gcgtagtctg aaggtttatt
    30721 ggcgcgatcc ggaatttcgc atgtttatcg gcgtacagtt tacgctggtg gttatttgta
    30781 ccctcgtact gtggtttcat aatgtctaca gttcggcgct gatgacaatt aaccaggcgt
    30841 ttttccaggt ggtgtcgatg gcgacaaccg ccgggtttac gactgacagc attgcccgct
    30901 ggccgctctt tttgccggta ctgcttttat gttcagcttt tatcggcggt tgtgccgggt
    30961 caacgggcgg tggcctgaaa gtgatccgca tcctgctgct gtttaagcag gggaaccgtg
    31021 aactgaaacg actggtgcat ccgaacgccg tctatagcat taagctgggg aatcgcgcac
    31081 tgccggaacg tatcctcgaa gccgtttggg gatttttctc cgcctatgca ttggtgttta
    31141 ttgtcagtat gctggcgatt atcgccacgg gcgtggatga cttttctgcc tttgcctcgg
    31201 ttgttgcgac attgaataac ctggggccag ggcttggcgt ggttgctgat aactttacca
    31261 gtatgaaccc ggtggctaaa tggatcctga ttgccaacat gctgtttggt cgtctcgagg
    31321 tctttacatt gctggtgctc tttaccccga ctttctggcg tgaatgatgg agtaatacgt
    31381 gaaaacatta attcttttct caacaaggga cggacaaacg cgcgagattg cctcctactt
    31441 ggcttcggaa ctgaaagaac tggggatcca ggcggatgtc gccaatgtgc accgcattga
    31501 agaaccacag tgggaaaact acgaccgtgt ggtcattggt gcttctattc gctatggtca
    31561 ctaccactct gcgttccagg aatttgtcaa aaagcatgcg acgcggctga attcgatgcc
    31621 gagcgcgttt tactccgtaa acctggtggc gcgtaaaccg gagaagcgca ctccacagac
    31681 caacagctac gcgcggaagt ttctgatgaa ctcgcaatgg cgtcccgatc gctgcgcggt
    31741 cattgccggg gcgctgcgtt acccacgtta tcgctggtac gaccgtttta tgatcaagct
    31801 gattatgaag atgtcaggcg gtgaaacgga tacgcgcaaa gaagttgtct ataccgattg
    31861 ggagcaggtg gcgaatttcg cccgagaaat cgcccattta accgacaaac cgacgctgaa
    31921 ataagcataa agaataaaaa atgcgcggtc agaaaattat tttaaatttc cccttgtcag
    31981 gccggaataa ctccctataa tgcgccacca ctgacacgga acaacggcaa acacgccgcc
    32041 gggtcagcgg gattctcctg agaattccg
//
LOCUS       DBFIFI010000036        31366 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.036, whole genome shotgun sequence.
ACCESSION   DBFIFI010000036 DBFIFI010000000
VERSION     DBFIFI010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.036"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..1256
                     /locus_tag="N7B39_001567"
     CDS             <1..1256
                     /locus_tag="N7B39_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HGU2421573.1"
                     /translation="AGFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLCGA
                     LPFGIVCVLAYSTPDLSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRI
                     SLQSWRFVLATAGGMLSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFT
                     TKERVEAPPTTTSMREDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGT
                     PEVFVAFLTTYCVGNLIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASI
                     TMFVFIFVIGVLHQLVTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFG
                     GALIGWMLAYGGYDAAEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNL
                     KTVMEQLAQGKRRCQQQFTSQEVQN"
     gene            1266..3584
                     /gene="yicI"
                     /locus_tag="N7B39_001568"
     CDS             1266..3584
                     /gene="yicI"
                     /locus_tag="N7B39_001568"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HGU2421574.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAGRNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTALAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFSEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHSFLSLPVYVRDNTLLALGNNEQRPDYAW
                     HEGTAFQLFNLQDGHEAICEVPAADGSVLFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     IVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(3637..5346)
                     /locus_tag="N7B39_001569"
     CDS             complement(3637..5346)
                     /locus_tag="N7B39_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HGU2421575.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTTLDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSSEVTSTE
                     PVQ"
     gene            complement(5467..6858)
                     /gene="xanP"
                     /locus_tag="N7B39_001570"
     CDS             complement(5467..6858)
                     /gene="xanP"
                     /locus_tag="N7B39_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HGU2421576.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            7138..8343
                     /gene="gltS"
                     /locus_tag="N7B39_001571"
     CDS             7138..8343
                     /gene="gltS"
                     /locus_tag="N7B39_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HGU2421577.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            8346..9212
                     /locus_tag="N7B39_001572"
     CDS             8346..9212
                     /locus_tag="N7B39_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2421578.1"
                     /translation="MKKRRKEPETLREHCRHIFGDEPPVLCVWETEFDYADAELKALA
                     AKEWQQISEWDLSAYYVLNLVYNEPMQIELFRYLFPLCLAQWHETVLAGGYGDHFEES
                     LMKALCRPYLWQEMMNASQRQQVRQFLLDTALQRMDNERGFNNVLCWLAVFNTLGGAA
                     PLIRSLWSRWWALDTPGKAVCAIQYAAHLIYPIEANPLWSQEWIGWGHPLGHKDGWSS
                     DNRAFLRQMLTPEMIVAGVQAAAEILRGEPEGAMAARIAQDAYEAMDILTIQIEDLLR
                     DLSCDESGHALE"
     gene            complement(9197..11278)
                     /gene="recG"
                     /locus_tag="N7B39_001573"
     CDS             complement(9197..11278)
                     /gene="recG"
                     /locus_tag="N7B39_001573"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HGU2421579.1"
                     /translation="MTGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(11284..11973)
                     /gene="trmH"
                     /locus_tag="N7B39_001574"
     CDS             complement(11284..11973)
                     /gene="trmH"
                     /locus_tag="N7B39_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HGU2421580.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(11980..14088)
                     /gene="spoT"
                     /locus_tag="N7B39_001575"
     CDS             complement(11980..14088)
                     /gene="spoT"
                     /locus_tag="N7B39_001575"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HGU2421581.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(14107..14382)
                     /gene="rpoZ"
                     /locus_tag="N7B39_001576"
     CDS             complement(14107..14382)
                     /gene="rpoZ"
                     /locus_tag="N7B39_001576"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HGU2421582.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(14437..15060)
                     /gene="gmk"
                     /locus_tag="N7B39_001577"
     CDS             complement(14437..15060)
                     /gene="gmk"
                     /locus_tag="N7B39_001577"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HGU2421583.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRDQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            15318..17000
                     /gene="ligB"
                     /locus_tag="N7B39_001578"
     CDS             15318..17000
                     /gene="ligB"
                     /locus_tag="N7B39_001578"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HGU2421584.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEIFRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAKVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHRFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(16997..17614)
                     /locus_tag="N7B39_001579"
     CDS             complement(16997..17614)
                     /locus_tag="N7B39_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HGU2421585.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(17906..18730)
                     /gene="dinD"
                     /locus_tag="N7B39_001580"
     CDS             complement(17906..18730)
                     /gene="dinD"
                     /locus_tag="N7B39_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HGU2421586.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAAVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            complement(18951..19814)
                     /locus_tag="N7B39_001581"
     CDS             complement(18951..19814)
                     /locus_tag="N7B39_001581"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HGU2421587.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            19941..20657
                     /gene="rph"
                     /locus_tag="N7B39_001582"
     CDS             19941..20657
                     /gene="rph"
                     /locus_tag="N7B39_001582"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HGU2421588.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            20723..21364
                     /gene="pyrE"
                     /locus_tag="N7B39_001583"
     CDS             20723..21364
                     /gene="pyrE"
                     /locus_tag="N7B39_001583"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HGU2421589.1"
                     /translation="MKPYQRQFIEFALGKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGLEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(21401..21997)
                     /gene="slmA"
                     /locus_tag="N7B39_001584"
     CDS             complement(21401..21997)
                     /gene="slmA"
                     /locus_tag="N7B39_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HGU2421590.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(22104..22562)
                     /gene="dut"
                     /locus_tag="N7B39_001585"
     CDS             complement(22104..22562)
                     /gene="dut"
                     /locus_tag="N7B39_001585"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HGU2421591.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(22540..23760)
                     /gene="coaBC"
                     /locus_tag="N7B39_001586"
     CDS             complement(22540..23760)
                     /gene="coaBC"
                     /locus_tag="N7B39_001586"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HGU2421592.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            23932..24600
                     /gene="radC"
                     /locus_tag="N7B39_001587"
     CDS             23932..24600
                     /gene="radC"
                     /locus_tag="N7B39_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HGU2421593.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            24817..25053
                     /gene="rpmB"
                     /locus_tag="N7B39_001588"
     CDS             24817..25053
                     /gene="rpmB"
                     /locus_tag="N7B39_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HGU2421594.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            25074..25241
                     /gene="rpmG"
                     /locus_tag="N7B39_001589"
     CDS             25074..25241
                     /gene="rpmG"
                     /locus_tag="N7B39_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HGU2421595.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            25339..26148
                     /gene="mutM"
                     /locus_tag="N7B39_001590"
     CDS             25339..26148
                     /gene="mutM"
                     /locus_tag="N7B39_001590"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HGU2421596.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(26187..26666)
                     /gene="coaD"
                     /locus_tag="N7B39_001591"
     CDS             complement(26187..26666)
                     /gene="coaD"
                     /locus_tag="N7B39_001591"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HGU2421597.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(26674..27951)
                     /gene="waaA"
                     /locus_tag="N7B39_001592"
     CDS             complement(26674..27951)
                     /gene="waaA"
                     /locus_tag="N7B39_001592"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HGU2421598.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            28364..29422
                     /gene="rfaQ"
                     /locus_tag="N7B39_001593"
     CDS             28364..29422
                     /gene="rfaQ"
                     /locus_tag="N7B39_001593"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HGU2421599.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            29419..30543
                     /locus_tag="N7B39_001594"
     CDS             29419..30543
                     /locus_tag="N7B39_001594"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HGU2421600.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYHLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            30536..31333
                     /gene="rfaP"
                     /locus_tag="N7B39_001595"
     CDS             30536..31333
                     /gene="rfaP"
                     /locus_tag="N7B39_001595"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HGU2421601.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVHDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
BASE COUNT         7355 a         7813 c         8454 g         7744 t
ORIGIN      
        1 ctgccggatt tgtcggaacc atgtttttgg tcgctcgtgc actggatgcg atttccgatc
       61 cttgcatggg gttgttggcc gatcgaacgc gctctcgctg gggtaaattt cgtccgtggg
      121 tactgtgtgg cgcactgcca tttgggatcg tctgtgtact ggcctatagc acgccagatc
      181 tcagtatgaa cggcaaaatg atctatgcag caattactta caccctactt accttacttt
      241 ataccgtcgt caatatccct tactgcgcat tgggtggtgt aatcaccaat gacccgactc
      301 agcgtatctc gctgcaatcc tggcgttttg tgctggcgac cgcgggaggc atgctctcta
      361 ctgttctgat gatgccactg gttaatttaa ttggcggtga taataaacca ctcggtttcc
      421 agggcggtat cgcggtcctt tccgtggtgg cattcatgat gctggcattc tgtttcttca
      481 ccactaaaga acgcgttgaa gcaccaccta caacgacgtc tatgcgggaa gatttacgtg
      541 atatctggca aaacgaccag tggcggattg tcggtttact aaccattttc aatatcctgg
      601 cggtgtgcgt ccgcggtgga gcgatgatgt attacgtcac atggattttg ggtacgccgg
      661 aagtgtttgt cgcttttctc accacttatt gcgtgggtaa cctgattggt tccgcactgg
      721 caaaaccgct gaccgactgg aaatgtaaag tcactatctt ctggtggacg aacgccctgc
      781 tggcagtgat tagcctcgcg atgttctttg ttcctatgca ggccagcatc actatgtttg
      841 tcttcatctt cgtgattggt gtattgcatc aactggtgac acctatccag tgggtaatga
      901 tgtccgatac cgtcgactac ggcgagtggt gcaatggtaa acgcctgacc gggatcagtt
      961 ttgctggcac gctgtttgtg ctcaaactgg ggttggcctt cggcggcgct cttatcggct
     1021 ggatgctggc ttatggcgga tatgatgcgg cagaaaaagc gcagaacagc gccacgatta
     1081 gcatcattat tgcgctattc acgattgttc cggcgatctg ttatttgctg agcgcgatta
     1141 tcgctaaacg ctactactca ctcacgacgc acaatctgaa aaccgttatg gaacagctgg
     1201 cccagggcaa acgccgttgc cagcaacaat tcacctctca agaagtgcag aactaaggaa
     1261 cggcaatgaa aattagcgac ggaaactggt tgattcaacc tggcctcaat ttgattcacc
     1321 cgcttcaggt gttcgaggtt gaacagcagg gtaatgaaat ggtggtctat gctgcccccc
     1381 gtgatgtgcg tgaacgaacc tggcagcttg atacgccttt atttacgctg cgctttttct
     1441 ccccacagga aggtattgtc ggtgtacgga ttgagcattt tcagggggcg ctgaataacg
     1501 gtcctcatta tccgctcaat attttgcagg acgtgaaggt cacaatcgaa aacacagaac
     1561 gttacgctga gtttaaaagt ggcaacttaa gcgcgcgtgt cagcaaaggt gagttctggt
     1621 cactggattt tctgcgcaac ggcgaacgta ttaccggtag tcaggtgaaa aataatggct
     1681 acgtgcagga cacgaataat caacgaaatt atatgtttga gcggctggat cttggcgttg
     1741 gcgaaacagt ttacggtctg ggagagcgct ttactgccct ggtgcgcaat ggccagacgg
     1801 tagagacctg gaaccgggac ggcggcacaa gtactgaaca ggcgtataaa aatatcccgt
     1861 tctatatgac taaccgtggc tatggggtac tggtcaatca tcctcaatgc gtctcttttg
     1921 aagtgggatc ggagaaagtc tccaaagtgc agttcagcgt tgagagtgaa tatctcgaat
     1981 actttgttat cgacggcccg acgccgaaag cggtacttga tcgttatacc cgttttactg
     2041 gtcgtccggc gctgccgccc gcgtggtcct tcggcctgtg gctaaccact tcatttacca
     2101 ccaactacga cgaagcgacg gtaaacagct ttatcgatgg tatggcggga cgcaatctgc
     2161 cactgcatgt ttttcacttt gactgtttct ggatgaaagc cttccagtgg tgcgattttg
     2221 agtgggaccc gctgactttc ccggacccgg aagggatgat ccgccgcctg aaagcgaaag
     2281 ggctaaaaat ctgcgtctgg attaacccct atatcgggca aaaatcccct gtctttaaag
     2341 agttacaaga gaaaggctat ttactcaaac gcccggacgg ttcgttatgg cagtgggata
     2401 aatggcagcc aggtctggcg atttatgact ttaccaatcc ggatgcctgc aaatggtacg
     2461 ccgacaaact gaaaggtctg gtcgcgatgg gcgttgattg ctttaagacc gactttggcg
     2521 aacgtattcc aatcgatgtt cagtggtttg acggttccga tccgcagaaa atgcataacc
     2581 attatgcgta catctacaac gaactggtgt ggaacgtgct caaggacacc gttggcgagg
     2641 aagaagccgt cttgtttgct cgctcggcct ccgttggtgc gcagaaattc ccggtacact
     2701 ggggtggtga ctgttacgct aactacgaat caatggcgga aagcctgcgc ggtggtttgt
     2761 ctattggcct ttcaggtttt ggtttctgga gccacgatat cggcggcttt gagaataccg
     2821 ctctggcgca cgtttacaaa cgctggtgcg cgtttggttt gctctccagc catagccgtt
     2881 tacatggcag caaatcttat cgtgtgccgt gggcctacga tgatgagtcc tgtgatgtgg
     2941 tgcgcttctt cacgcaactg aaatgccgca tgatgccgta tctgtatcgt gaagctgctc
     3001 gtgcgaacgc gcggggtacg ccgatgatgc gggccatgat gatggagttc ccggacgatc
     3061 cggcttgtga ttaccttgac cgtcaataca tgttaggcga caacgtgatg gttgctccgg
     3121 tgttcagtga agcgggcgat gtgcagttct acttgccgga aggtcgctgg acacatctgt
     3181 ggcacaacga tgaactcgat ggtagtcgct ggcataaaca gcagcacagc ttcctgagtc
     3241 tgcccgttta tgtgcgtgat aacaccctac tggcgctggg caacaacgag caacgtcccg
     3301 attacgcgtg gcacgaaggc acggcattcc agctctttaa tctacaagac gggcatgaag
     3361 ccatctgtga agtgcccgct gctgacggtt ccgttctttt caccctgaaa gcggcgcgta
     3421 ctggcaacac gattactgtg actggtgcgg gcgaggcgaa gaactggaca ctgtgcttgc
     3481 gcaatattgt gaaagtaaat ggtctgcaag gtggttcgca ggctgaaagt gagcaggggc
     3541 tggtggtgac gcctcaaggg aatgcgctga caattacgtt gtaatctatt gatggaatgc
     3601 cgggtacgtc tgagtcgtac ccggcgagat tgatttttac tgcaccggct ctgtcgatgt
     3661 aacttcgcta ctggaaacga cgcccgcatt catggtttga gtcacttgct gctttgcggc
     3721 gttcacggca tggagttgcc cgctaaccgt aggtttcagc gggacattgg cctgaatatc
     3781 gccactggcg gtaagctgaa tattgccgtc gccagtcaac ggtaattcag gccatcccca
     3841 ttgttgcaaa atattcaccg gcacaccgcg tccattcagg ctgatatggg tctgacgttg
     3901 tggcgtttgt gaaacactgg cagtggcttc cagaatgcct ttttcagtaa atgcactcag
     3961 ttcgctgata ttcaccgtgc tgctgttggc ggtcagcgcc agcgacgggc gacgaacatc
     4021 aacacgattg aatgtcgcgg cggcggcatt cagattcgcc gagccactcc agacgcccca
     4081 tttatgatcg gtaaccagcg tcaggttggc accgtaacca tcgagcgtgg tgagctgcca
     4141 cgggaagtca gggtcgatat caatgatcag attgcggctg gcgctaaatc tcttcagttg
     4201 caggctgttt aaccaaccgg gtgtcgtttc catccacaac tgttgccagt ttttcggcaa
     4261 ggtatattcc agcccggcaa ttgccgcatc atcaaggatc aacgttttcc cgtcacgcag
     4321 ccagttccct gacgttctga ccataccccc ttcccagcgg ctggtgaact ggcgcagcgc
     4381 tacgccctgc ggggaaaatt ccgcgtttat aatcgggtca aataaatgca gcgaaccata
     4441 aatgaactcg ctagcgttca tcgacagttt gccttcttgt gtctgccagt catctttact
     4501 gaaggtcatg ttgcgcaagc tgagatcgag gtcggtcacc gcccagtccg gaccttgcaa
     4561 acgagcatcg atcacttcca ggcgaccaat ctgcaacgac gggacagagc gtaatggcgc
     4621 aaagaagtcg gttagcgatt tttcgctttg tagacggata tccgccatgc gcagattttc
     4681 cacttgccag ctgccgtcgg cgttacgctg cgcgtttccg gttaatgtcc cgcgggcgat
     4741 gtcggcaccc aggttagtca gcgtaacgcg atcgttatca atactgcctt caatcagtac
     4801 attggtggca ggaacatcat tgagcgaaag cgatccggca ctaaactgaa tctgcgcctt
     4861 cgtacccagc actttaccgg cttctggtga ccacggaacc acgccgccat ttacccgctg
     4921 cgcgctcagt ttccattcgc tattcgggct attaaacgcc atatcacgca gttgcagacg
     4981 atcggctttg aacggtagcg gcgcggtctg gtcggtgaga ttcagcgtcc cgttttccag
     5041 caggatggta tcgacatggc gtggttcggt cagttgccga ctgcttagcg caatgtcgac
     5101 actttttgcc accagggtcg cgggctgacc atcacgacca aacgtgacgt tctccagcac
     5161 gatatgagat ggcgcggaaa aacggtgatc catcgccccg aaggccagat gatagtcgct
     5221 attctcggaa acccatgcgc tgatatgttc tgctccccag cgggtttgca gaagaaaata
     5281 gaggccagcg atcaccacta acagagcgat gagaatgtag agaagcagct tcccaataaa
     5341 tttcatggtc ttccatcccg tgaaatgcac ataagggagt tatgcacgat ttacgcgcaa
     5401 tcctcaaggc gggaatggtg aaagaggtga gactgcggcg gtaattatct taccgccgcc
     5461 agtgaattac tgtttttctg gtgggaaaat cagattcagc acgatggcag taataccgcc
     5521 cgcggcgatc ccggaggaga gcaggttttt cagccattca ggggcaaact gcaaaatcag
     5581 cggctgctga gacacgccca gaccaaccgc cagcgacagc gcgataatca gaatcgcccg
     5641 acggttcagc ggctcacgag aaacgatacg cacaccggag gcggcgatgg tgccaaacat
     5701 tacaagcgtt gcgccgccca gaaccggttc tggaatgtgt tgtacaaaac cgctcactgc
     5761 cgggaacaga cccagcacga tcaacatcag cgcgacgaca aaaccgacat agcggctggc
     5821 aacaccagtc aactggatca ctccgttgtt ttgcccgaag caggagttcg ggaaggtgtt
     5881 aaacaccgcc gaaacaaacg agttcaggcc gtttgccagc acgccgcctt tcaggcgttt
     5941 catgtacagc ggaccggaca ctggctgttc ggaaacgtca gaggtcgccg tgatatcgcc
     6001 aatggtttcc agcgaagtga tcataaagac cagcatcagc ggcagcagca gactccattc
     6061 aatgccaaga ccgtaataga gcggcgttgg caccataatc agttcttgcg tcatcggttc
     6121 gttgctttct ggcaacatgc ccataaacca cgccagcgca tatccggccg ccatcgcaat
     6181 caccagtgag gccacgcgta agtaagggtt acgttgacgg ttaagcagga taattaaggc
     6241 taagaccacg cctgccagca gcagattttt cggtgcgccg aaggtgttat cgctcatggc
     6301 tgcgtaaccg ccgccaatgg acgttaaccc aacctgaatt agcgacaggc cgataatcat
     6361 caccacaacg ccagaaacca gcggcgtaat aatgcggcgc gccagatgca gaacgcggga
     6421 gatcaccatc tcggtgcaac ttgccagcat caacgtgccg aacaaagccg ccatcatggt
     6481 aggaacatca gcaccaccgg ttttcagcgc ggtaccgccc ataatcagcg gggcaacaaa
     6541 gttgaagctg gtgccctgaa tagacaacag cccggagcca accggacccc aggccttaat
     6601 ttgaataatc gatgccacac cggaggcaaa cagcgacata ctaataatgt gttgcgtgtc
     6661 ttgtgccggt aaacccagcg cctggcagat taatagcgct ggcgtgatca ccgcaacgaa
     6721 catcgccagc agatgctgac aggcggcaaa cagggtttga ggaagcggcg gacgatcttc
     6781 aagacggtaa atcagttcgc tgttttgagt ctgcgcaacc ggttgcgcat tttctgactc
     6841 gagggtggaa acagacatcg aaaacaatcc cgtggtagca aagcgggcat tttagctgac
     6901 cgaaagagaa aagcaaacgt ttgccacatc ctcatctaaa tatttaaatg agataaatgc
     6961 acttttttat ataacttttg gtatttttct gcttaaaatc catgccattt gttcattaac
     7021 agctgagaat ttgacccagg aaagcataac tcgatacccg tcatagttca tgaagttgcc
     7081 actgcgcgat gaagtatgac gagtatgaaa gagtgatgcg gatacaaagg agtaactatg
     7141 tttcatctcg atactttagc aacgcttgtt gccgcaacgc tgacgttgtt gctcgggcgt
     7201 aagttggtcc attccgtctc ctttttgaag aaatacacca taccggaacc tgttgcgggt
     7261 ggtttgttgg tggcgctggc gctactagta ctgaaaaaaa gcatgggctg ggaagtcaac
     7321 tttgatatgt ccctgcgcga tccgttaatg ctggctttct tcgccaccat tggcctgaac
     7381 gccaacattg ccagtttgcg tgccggtggg cgtgtggttg gcatcttctt gattgtggtt
     7441 gttggtctgt tggtgatgca aaatgccatt ggcattggta tggctagcct gttagggctt
     7501 gatccgctga tggggctgtt ggccggttct attacgcttt ccggcggtca cggtacgggc
     7561 gctgcgtgga gtaaattgtt cattgaacgt tatggcttca ccaatgcgac ggaagtggcg
     7621 atggcctgtg caacgttcgg tctggtgctg ggcggcttga ttggcggtcc ggtggcgcgc
     7681 tatctggtga aacactccac cacgccgaac ggtattccgg atgaccagga agtcccgacg
     7741 gcgtttgaaa agccggatgt gggccgcatg atcacctcgc tggtgctgat tgaaactatc
     7801 gcgctgattg ctatctgcct gacggtgggg aaaattgttg cgcaactttt ggctggcact
     7861 gcttttgaac tgccgacctt cgtctgtgta ctgtttgttg gcgtgattct gagcaacggt
     7921 ctgtcaatga tgggctttta ccgcgtcttt gagcgtgcgg tatccgtgct gggtaacgta
     7981 agcctgtcgt tgttcctggc gatggcgctg atggggctga aactgtggga gctggcttcg
     8041 ctggcgctgc cgatgctggc gattctggtg gtacagacca tcttcatggc gttgtatgcc
     8101 atcttcgtta cctggcgcat gatgggcaaa aactacgatg cggcagtgct ggctgcgggt
     8161 cactgtggtt ttggcctcgg tgcaacgcca acggcaatcg ccaacatgca ggcgatcact
     8221 gaacgctttg gcccgtcgca catggcgttt ttggtggtgc cgatggtcgg tgcgttcttt
     8281 atcgatatcg tcaatgcgct ggtgattaag ctgtatttaa tgttgccgat ttttgccggt
     8341 taaaaatgaa aaagcgtcgg aaggaaccgg aaacgctacg tgaacactgt cgccatattt
     8401 ttggtgatga gccgccagtg ctctgtgtat gggaaacgga atttgattac gcagatgctg
     8461 aactcaaggc gctggcggct aaagagtggc agcagatcag cgaatgggat ttgagtgctt
     8521 actatgtact caatctcgtt tataacgaac cgatgcaaat agagttgttt cgctatctgt
     8581 ttccgctctg cctggcgcaa tggcatgaaa ccgttttagc tggcggttat ggtgatcact
     8641 tcgaagaatc cttaatgaag gcattgtgtc ggccctatct ctggcaggag atgatgaatg
     8701 cttcccaacg ccagcaggta cggcaatttt tactggacac cgcgttgcag cgtatggata
     8761 acgagcgtgg tttcaataac gttctttgct ggctggcggt gttcaatact ctgggtgggg
     8821 ccgcgccgtt aattcgttct ctctggtcgc gctggtgggc gctggatacg ccaggaaaag
     8881 cggtgtgtgc tattcagtat gccgcgcatc taatttatcc catagaagcc aatccgttat
     8941 ggtcgcaaga atggataggc tgggggcatc cattaggcca taaagatggc tggtcctctg
     9001 ataatcgcgc ttttcttcgc caaatgttaa cacctgaaat gattgtggct ggcgtacagg
     9061 cagcggctga aattctgcgt ggtgaaccgg aaggcgcaat ggcggcgcga attgcacagg
     9121 atgcgtatga ggcaatggat atcctcacta tccagattga agatttactg cgtgacttgt
     9181 cttgtgatga gtctggtcat gcgttggagt aacgttccgt ctccggcatc cagcgttcta
     9241 tcagggcttt tgcttgttgt gggtaacgtt cgtgaatatg tcgcgccagg cgctgaactt
     9301 ccgggatcat cgcctgatcg cgcagtaaat ccgccacttt aaactcagca ttacccgtct
     9361 gacgcgtacc taacagttca cccggtccgc gaatctctaa gtctttctgt gcaatcacaa
     9421 aaccgtcgtt gctgtcgcgc agcacttgca gccgaatttg cgcggttttg gagagcggcg
     9481 ttttgtagag cagcacgcag tgagaagcca ccgcaccacg acctacacgc ccgcgaagct
     9541 ggtgtaactg cgccagaccc agacgctcag ggttttcgat aatcatcagg ctggcgttag
     9601 gcacatcaac accgacttca ataacggtag tggcaactag cagatgtagc tcaccttgtt
     9661 taaacgacgc catcaccgcc tgtttctcgg ctggtttcat ccgcccgtgt accaggccaa
     9721 cattcaattc tggtagcgcc agtttcaact cttcccaggt agcttccgcc gcctgcgctt
     9781 ccagtaactc tgactcttca atcaacgtac aaacccagta cgcctgacga ccttcagtta
     9841 tgcaggcgtg gcgcacgcgg tcaatgatgt cggtacggcg ggtatcagga atagcgaccg
     9901 tagtcactgg cgtgcggcct ggcggcagct catctatcac cgaggtatcg agatcggcat
     9961 acgccgtcat tgccagcgtg cgggggatcg gcgtggcggt catgatcaac tgatgcggat
    10021 ggaagccttg ctgctggcct ttctcccata acgccagacg ctgatgcacg ccaaagcgat
    10081 gttgttcgtc gataatcacc agcgccagac cgttaaactg cacctgttcc tggaagatgg
    10141 cgtgcgtacc aacaatcatc tgcacctgac cgctggcgat ggcttcctgc tgagacagtc
    10201 gcgctttgcc tttctgctta ccggcgagcc agcccacttc gataccgagc ggttcaaacc
    10261 agttgcggaa gttattggcg tgctgttcgg cgagtaattc ggttggtgcc atcaatgcca
    10321 cctgtttgcc gtgggcaatc gcacgcagcg cggcgagggc ggcgaccagc gttttaccgg
    10381 aacctacatc gccctgtacc aggcgcatca tcggcacatc cagcgccata tcgcgctcga
    10441 tctccgccac tacgcgtgcc tgtgcgcccg ttggcttgaa cggtaaggct gcgaggagtt
    10501 tatttttcag cgcatcattg gcgctcagcg gctgggcatg aaaacgctgt gctccggcac
    10561 gtaaggctaa catgctgagg ttgtgcgcca gcagttcttc cagaatcaga cgacgttgcg
    10621 ctggatgctg cccggtttcc agatcgctaa gctgtagcgt cggtggcggg cggtgcaaag
    10681 tgcgcaacgc ttccggtagc gtcatcattc cttgtgacag ttccggcggc aggagttctt
    10741 caatggcgca ggtgtcgagc agatccagcg cctggtcggt taatttacgc agcgtggcct
    10801 gctttacgcc ttccgttgtt ggataaaccg gcgtgagcgt ttcctgtaat tctggcgtgc
    10861 tgagatcgcc ctgcacgcgg tattccgggt ggatcatctc cgcaccatat ttaccgcgct
    10921 ttgcttcgcc ataagccagt acacggcggc ccgtcgccag gctatttttc attgccgcgc
    10981 tgaaattgaa aaagcgcatg gtgaggatgc cggaaccgtc gctgatctgg caggtcatca
    11041 tccgccgacc gccgaaggag atattgcagt tcagcacttc gccttccacc gtggcataaa
    11101 cgcccggcag tagttctccg atggggtaga gatgggtgcg atcttcgtag cgcaggggaa
    11161 ggtgtaagag tagatcctgc acggtatgca ggttgatttt cgccagtttg ttactaagtg
    11221 ctgcgccaac gcccgttagg gaactgagtg ggacagcatc taacaggcga cctgtcatgg
    11281 cacttaccct gcagcctgca tagtagccca ccagtcggca tcagcttcga tctcgccttg
    11341 ctgattgacg tggggataag gcaggccttt gcgttttgcg actttcgcca gcaccggata
    11401 gccgccttca aacaacaggc gttgttgctc tgcttccggc aacatgctgt tttcacgcag
    11461 gtacatgcct gcattttgcc gctgacgctg ggcttcgtaa agaatgaggg ctgaggcaac
    11521 ggaaacattc agcgactgca ccatgccgat catcggaatg atgatgtcct gatccgccag
    11581 ggccaatgct tcctgcgtga tgcccgtttt ctcctgtccc atcaaaatgc aggtcgggcg
    11641 agtgtaatca atttcgcgga aatcgacagc gttatcagaa agatgggttg ccagaatctg
    11701 catgccctgg cctttgagat gagcgacggc atcgccaatg gtgcggtgtg ttttcacctg
    11761 tacccagctg ttactacccg ccgctgccga agccatggtg cgcatgcggc taccaggcca
    11821 gacggcgtga acttcatgta cgccaacggc atctgcggta cgaataatcg cagaaacgtt
    11881 atgaggtttg tggacctgct ccatgcagac ggtcagatca ggctgccgcc tggcgagcat
    11941 ttcgcagatg cgtgcataac gtgttgggtt cataaaacat taatttcggt ttcgggtgac
    12001 tttaatcacg tctggcatca cgcggatttt gcgcatgata ttcgccagat gcacacggtc
    12061 acgagcggtc agacgaataa aggcgctgta gacgcgacca tctttctctt ccgtattcaa
    12121 actttgaata ttcgaggtcg tggtgttaat tgccgccgtc aggtttgcca gcgcaccctg
    12181 atgattgaac atctccacct tgatttcggt gatgaactcc tgcgccgtct ctttatccca
    12241 ttccacagcc ataaacttct ctggctcttt ctggtagcca cggatgttac ggcaggattc
    12301 atggtggatc accagacctt taccggggct gacgtgggcg ataatcgggt cgccaggaat
    12361 ggggcggcag catttcgcaa aggtgatcag cacgccatca gcgcctttga tcggcagatg
    12421 cccgtggctt tgagtcgccg gtggaatgga ggcgtcccca tgttgtagat ttttcgcgac
    12481 caccacgctc attgcgttac caaggccgat ttctgccagc agatcgtcaa gcgttgccag
    12541 cttcatgcga tccagctcgc gctgaatatt ttcctgcggg atttcattga gcttacggct
    12601 accacccaaa gcatggttga gcagacgacg gcccaggctt acagaatcat cacgcttgag
    12661 gtttttcagc aactgacgaa ttttggcgcg cgctttcgag ctaacgacaa agttcagcca
    12721 agcggcattc gggcgagcgc ccggagcggt aatgatttca acggtctgac cgctagtaag
    12781 cggctgcgac agcgggtaag gctggcggtc gacgcgtgcg cccacgcagg catggccgat
    12841 atcggtatgc actgcataag cgaagtcgac gggcgttgca ccggcaggca gctcgacaat
    12901 gcgcccttcc ggtgtgaaaa cgtaaatctc atccgggaag agatcggatt taacgctctc
    12961 gataaattca aacgaactac cggcgctctg ttgcagctcc agcaggcttt gcatccagcg
    13021 ctgggcgcgg atttgtgcgg tagtactggt ttcgccgtgc tctttataag cccagtgcgc
    13081 ggcaacaccc atctccgcca tctgatccat atcttcggta cggatctgga cctcaaccgg
    13141 cacgccgtgc gggccaatca tcgaggtgtg caacgactga tagccgttcg cttttggaat
    13201 ggcgatatag tctttcacgc ggcccggacg cggcttgtac aggctgtgca tctggcccag
    13261 cacgcgataa caggtgtcag aatcattgac gatcacgcgg aaagcgtaga tgtccatgat
    13321 tgagtgaaaa cgctgctctt tgagcaccat tttacagtaa atcgaataaa gatgcttttc
    13381 gcgaccactg acgcggcacg gtattcccgc ttcctgcaaa cgcccttcga tttcagaaag
    13441 aatcttctgg atcatctctt tacggttgcc gcgcgcggct ttcaccactt ctttgattac
    13501 gcgataacgg ttgggataca gcgcctcaaa acccagctct tcgagttcgg ttttaatgtg
    13561 gtggatacct aaacggtgcg ccagcgggct gtaaatttcg agagtttcac gggcgatgcg
    13621 gcgacgtttg tccgggcgaa gtgagcccag cgtgcgcatg ttgtgggtac ggtcggcaag
    13681 tttgatgagg atgacgcgga tatcctgcac catcgccata atcatcttgc gaaagttttc
    13741 ggcctgcgcc tctttcttat cgcggaactt gagtttatca agtttcgaca ccccctctac
    13801 cagctcggcg acgcttttac caaaaagctg ttccatatcc tggtaggtgg cgggagtatc
    13861 ttcaatcacg tcatgcagca gcgccgccat cagcgtttca tagtcgagtt tcatctcggc
    13921 cagaatgcag gcaaccgcta ccgggtgcgt gatatagggt tcaccgcttg aacgtgtttg
    13981 cccctcgtga gcatcacgtg caacgagata cgcctgccgc agacgcttga tttggtcttc
    14041 cggcaggtag gtttgaatca gttgattcag gctttcaaac agatacaagg gcgacccgct
    14101 ttgtgattaa cgacgacctt cagcaatagc ggtaacggct tgtaattcag cggcttcctg
    14161 ctcttgctgt tcctggcgtt cgcgaacgtc gaggatctgg ttgttgatca gaccttcttc
    14221 gatttcgcgc agcgcgatta cagtggtttt atcgttttct tccggtacca gcggatcctt
    14281 tccgcctacc tgcatctgac gagcgcgacg cgcggcgacc agtaccaggt caaaacggtt
    14341 accaattttc tctacagcgt cctgaacagt tacgcgtgcc atacttaaaa agctccacag
    14401 gtgaagaaat gactgggcat gatactgaaa tcaggttcag tctgccaaca atttgctgat
    14461 taaagcgtca tgacgctgct tttggcggct catgcgcaga cgttcggcgc gaataatggt
    14521 cttcaaatcg gtcaacgcgg tatcgaagtc atcattcaca atcaggtaat catattcggc
    14581 gtaatggctc atttctgcaa cagcttgcgc catacgcttt gcaatgacct cttcgctgtc
    14641 ctgatcgcga ccgcgtagac ggcggtccag ttcaatttta gacggcggta aaataaagat
    14701 actccgcgcg tgcggcatct tctggcgaat ttgctgcgcg ccctgccagt cgatatcgag
    14761 aaaaacatcg acaccggtcg ccagtacttg ctcaatggcc tcacgcgaag tgccatagta
    14821 attaccaaaa acttctgcgt gttcgaggaa cgcatctctg ctaatcattt ctttaaattc
    14881 atcatgatta acaaagaaat aatgttcacc gtggacttca ccaggacgcg gttggcgtgt
    14941 ggtgtgtgaa acagaaacct gggtgtcata caacggttgg gtttttaata aagcctgaat
    15001 caggctggat ttacccgcgc cactgggggc agaaacaata taaagcgtgc cttgagccat
    15061 gagtatcttt cgtatgtgat tagcgaaata aagcctacat acgagcttat tatacacggc
    15121 gcggccatgt gacgtagccc ttgctcacac tttttgcgca aagttgttgc gttttgcgta
    15181 gctgtttccg catttgcctg tgatttactg caataaagac gattctcagg aagcgtcctc
    15241 gcagactccg tttttgccac tcggctttgc cttcaccacg ttgtatatct gccagcctga
    15301 ctacagcgga ggtgaagatg aaagtatgga tggcgatatt aataagtatc ttgtgctggc
    15361 aatcatctgt gtgggcggtc tgtccggcct ggtcgccagc cagagcacag gaagaaattt
    15421 tccgcctgca acagcaaata aaacagtggg acgatgacta ctggaaggaa ggaaaaagtg
    15481 aggtggaaga cggtgtttac gatcagttaa gcgcccgtct tacgcagtgg caacgctgct
    15541 ttgtgagcga gccccgcgac gtgatgatgc cgcctttaaa tggtgcggtt atgcatccgg
    15601 ttgcccatac gggtgtacgc aaaatggcgg ataaaaacgc attaagtctg tggatgcgag
    15661 aacgtagcga tctttgggtg cagccaaaag ttgatggcgt tgcggtaacc ctggtttatc
    15721 gggacgggaa actgaacaaa gcaatcagtc gcggtaatgg cctgaaaggc gaggactgga
    15781 cgcagaaagt tagcttaatt tccgctgtgc cgcaaaccgt tagcgggcct ttagccaaca
    15841 gtacgcttca gggggaaata tttctccagc gcgaggggca tatccaacaa caaatggggg
    15901 gaataaatgc ccgcgcaaaa gttgctggcc tgatgatgcg ccaggacgat agcgacacgc
    15961 tgaattctct gggcgttttt gtctgggcat ggccggatgg accgcagtta atgaccgatc
    16021 gtttaaagga gctggctacc gcaggtttta ctctaacgca gaggtatacc cgtgcggtta
    16081 aaaatgctga tgaggttgcg cgcgtacgca atgagtggtg gaaagcgaaa ttacccttcg
    16141 tcaccgatgg cgtggttgtg cgaggggcga aagaacccga atcccgccat tggctaccag
    16201 gccaggcaga atggctggtg gcctggaaat atcaacctgt agctcaggtt gccaaagtga
    16261 aggcgattca gtttgcggtg ggtaagagcg gtaaaatatc ggtggttgcg tcactcgcac
    16321 ctgtcatgct ggatgataaa aaagtccagc gggtgaatat tggttccgtc aggcgctggc
    16381 aggagtggga tattgcgcct ggtgatcaga tcctcgtcag ccttgccggt cagggtattc
    16441 ctcgcattga tgatgtggtc tggcgcggtg cagaacgtac aaaaccgaca ccaccagaaa
    16501 accgctttaa ctcgttgacc tgctactttg cttctgatgt ttgtcaggaa cagttcattt
    16561 cacgcttagt ctggctgggg tcaaaacagg ttctggggct ggatggcatt ggtgaggccg
    16621 gctggcgcgc gctgcatcag actcatcgct ttgagcatat cttttcctgg cttttattaa
    16681 cgccagagca attacagaac acgccgggga tcgcgaaaag taaaagtgcg cagctatggc
    16741 atcggtttaa tctggctcgt aagcagcctt ttactcgctg ggtgatggca atgggaatac
    16801 cgctaacccg ggcggcgctt aatgccagtg atgaacggtc ctggtcgcaa cttttattta
    16861 gcacggagca gttctggcag cagctgccgg ggactggatc ggggcgcgcc agacaggtta
    16921 ttgaatggaa ggaaaatgcg caaatcaaga agctgggcag ttggctggct gcccagcaga
    16981 tcacaggttt tgaaccttag tgtccttcgt ggctgtagta aaagaccggt aatcccagct
    17041 taaggcgtag cgccagtaag cgggcaaaaa agccgaatac cagggtggag ataattacca
    17101 catcatggtt agaaacatag tgttgcagcg cgatgtacag cacggcggag gcaaatgaga
    17161 caccggcata caactctttc tggaatacca gtgggatacg tttacagaac atatcgcgca
    17221 aaacgccgcc aaacacgccg gtggttaccg ccgcgacaac ggcaataatt gggccgtgcc
    17281 ccatatccag tgcaacctgt gcgccgatga tagaaaagac caccagtcca agcgcatcga
    17341 gcaccagaaa cactttgcgc aggtaaggca ttacaggagc aacgatagtg gtgagtactg
    17401 cggcggtagc gacgataatc acatattccg ggtgtttgac ccagccgagc ggatagtggc
    17461 ccagcagaat atcgcgcact gaccctccgc caattgcggt ggcggtcgca ataataatta
    17521 cgccaaatgt atccatgcgg cgtcgcccgg ccgccagagc ccccgtcatg gcttcggcag
    17581 taatgccaac caaatacaaa atgtgtaaca gcatggttct ctccctaaaa atctgtctgg
    17641 cagggtagcg atttgtgcgt attgtcacga ttgagatttt ttaaggtgag aggaaattga
    17701 taacttgtag ttatgtgaaa tcgctatttt ctgtagcgta gatgcattct tctgacttct
    17761 tgtctgagaa aatgcataag aaaaaatagg ttaaaattga ggggtggaaa agaaaggtgc
    17821 aatgtttagc cattgtgttc accgcacctg gactttggct tacgcatcgg gtatctcaat
    17881 caacaaaaag agtttttagt ttctgctatt tcttctctgt aatttttaca ctgttttcca
    17941 actgcttgat gctgacctgc ggggtcggca actcctcagg catggttccg ccaagttcct
    18001 gaatggtttg cctcaccttg ctacccacat caaagtgtgt ggtattagct tgctgttttg
    18061 aattcacgcc atcccgcttg agtttttctt ctgtttgggt agctcgaaag agattagccg
    18121 ccagttctgt tgaacccata tgatccagga tcttctgact ctttttcagc cccttcagtt
    18181 ggtggatggc tttctgatct aatccaccat acagcccctg gtaaccatga ttctgaaata
    18241 tggcgaaatc agtagctgtc gctaccgcag cttgctgtgc tgcctcaacc aattgtttgt
    18301 tatgttcttt caactcattt ctcagaaaca gacgcttttc gtcttcacga agttgcttaa
    18361 atgcttcatc gtcagcaagc tcctgccgtc gggtctggat agcaaaataa gtctgccctg
    18421 ccgcaatgac cggtttcgca gggtcgccgt tttgtactac caggtaacag gcatagcggg
    18481 agagatgaac atcttcaagt tctcgttgag cccctgaacc taatacaacc attttggtgg
    18541 tttcaacgaa atggtcagat gcagcttgat tgctggcttc gcacgcttga gtcgcgcggg
    18601 ccagtacctt ttgaaaattg cgccagtccc gataatccag taacggtgcc aattctctag
    18661 ctgaccaaaa ttcttgccct tcagtgccat aatgccgtat ttcttcaaaa ggttgatgat
    18721 gttcgttcat tgcccactcc atgtgatatc gattgcaaag aaagtaaatc tgtaactgta
    18781 tttatataca gttgtgagta tctcacgttc tcagtgtgct gggcaaaaaa acatcacaaa
    18841 atctggagca ggcacgcaaa acagaattga agttgcgggg aagaaaaatg ccgctctgtt
    18901 tgagtgggcg gcatttgtga aggctgaaaa gtaagaattg gcgttacgag ttattcaatg
    18961 ttctgaatct gctcgcgcat ctgctcaatc aacactttca gctcaatcgc ggagtttgtc
    19021 acttcggcat tgatagactt cgacgcaaga gtgttcgact cgcggttgaa ttcctgcatc
    19081 ataaagtcca gacgacgacc aacggcttct tttttcttca gaatgttgta ggtctctttg
    19141 acatgcgctt ccaggcggtc cagttcttcg gcaacgtcaa ttcgttgtgc cagcagaacc
    19201 agctcctgct ccagacggtt gttttccaac tgaacctgag catcttccag cttcgcgacc
    19261 agacgctcac gctgccattg caggatttcc ggcatatggg cgcggacttt gaccacttcg
    19321 gcggtgacgc cttccagacg ctgctcgatc aatgctttca gtgcctgacc ttcggtttcg
    19381 cgcgcgacaa taaagtcgtc cagcgtacca tccagcgccg cgagaatttc agcggcaatg
    19441 gcgtcaagat cctgctcctg ggctgccatc acgcccggcc agcgtagaat atcaaccggg
    19501 ttgatttccc cttcgtcact ctgcattttt acccagttcg cggcagttac cagctgttta
    19561 gccagttttt cgttgaggat cagctcacct tgcgcgctaa catctggctc atagcgcagg
    19621 gtacattcca ctttaccgcg cgtcaggcga gaacgaatac gctcgcgaac gacaggttca
    19681 aggctacgga actgctccgg cagacgaaag taagtttcca gataacgctg gtttaccgag
    19741 cgcatttccc aggttgcgct cccccattca cccttgattt cacgccgggc gtaggcggtc
    19801 atactgcgga tcatagacgt tcctgtttat aaaaggagag gtggaaggat tatagccatc
    19861 gatgccttgt caggatagga ataaccgccg gaagtccgta taatgcgcag ccacatttgt
    19921 ttcaagccgg agatttcaat atgcgtccag caggccgtag caataatcag gtgcgtcccg
    19981 ttaccctgac tcgtaactat acaaaacatg cagaaggctc ggtgctggtc gaatttggcg
    20041 ataccaaagt gttgtgtacc gcctctattg aagaaggcgt gccgcgcttc ctgaaaggtc
    20101 agggccaggg ctggatcacc gcagagtacg gcatgctgcc acgttctacc cacacccgta
    20161 acgctcgtga agcggcgaaa ggtaagcagg gtggacgcac aatggaaatc cagcgtctga
    20221 tcgcccgtgc tcttcgcgcg gcagtagatt tgaaagcgct gggtgagttc accattacgc
    20281 tggactgcga cgtgcttcag gctgatggtg gcacgcgtac cgcgtcgatt acgggtgcct
    20341 gcgtggcgct ggcagatgcg ctacagaagc tggtggaaaa cggcaagctg aaaaccaacc
    20401 cgatgaaagg gatggtagcc gcagtttctg tcggaattgt gaacggcgaa gcggtttgcg
    20461 atctggaata cgttgaagac tctgccgcag agaccgacat gaacgtagtg atgaccgaag
    20521 acgggcgcat cattgaagtg caggggacgg cagaaggcga gccgttcacc catgaagagc
    20581 tactcacctt gttggctctg gcccgagggg gaatcgaatc cattgtagcg acgcagaagg
    20641 cggcgctggc aaactgattt ttaaggcgac tgatgagtcg cctttttttt gtctgtagaa
    20701 aagtaagatg aggagcgaag gcatgaaacc atatcagcgc cagtttattg aatttgcgct
    20761 tggcaagcag gtgttaaagt ttggcgagtt tacgttgaag tccgggcgca aaagccccta
    20821 tttcttcaac gccgggctgt ttaataccgg gcgcgatctg gcactgttag gccgttttta
    20881 cgctgaagcg ttggtggatt ccggccttga gttcgatctg ctgtttggcc ctgcttacaa
    20941 agggatcccg attgccacca ctaccgccgt ggcgctggcg gagcatcatg accttgacct
    21001 gccgtactgc tttaaccgca aagaggcaaa agaccacggt gaaggcggca atctggttgg
    21061 tagcgcgtta caaggacgcg taatgctggt agatgatgtg atcaccgccg gaacggcgat
    21121 tcgcgaatcg atggagatta ttcaggctaa tggcgcgacg cttgctggcg tgttgatttc
    21181 gcttgatcgt caggaacggg ggcgcggcga gatttcggcg attcaggaag ttgagcgtga
    21241 ttacaactgc aaagtgatct ctatcatcac cctgaaagac ctgattgctt acctggaaga
    21301 gaagccggaa atggcggaac atctggcggc ggttaaggcc tatcgcgaag agtttggcgt
    21361 ttaaagaaac tcgccggatg aaaagtcatc cggcgtcata ttactgcaac tgtgccgcaa
    21421 ttagcggcca gcgggcgtca aaatcatccg tcgggcggta tttaaattcg ctgcggacaa
    21481 aacgtgacag cataccttca cagaaggcta ggatctggct tgccagcagg gtttcatcgg
    21541 tggtgtaacc ttcaccctca cgcattctct tttcacgcaa tacctgacgc agctgcgctt
    21601 caatacgctc gaacagctgg ttgatgcgcc cttgcaggcg atcctgttca aacattagcg
    21661 catgaccagt gaggatgcgg gtcaggccag gattacgctc accaaaaccg agaagcagca
    21721 acacaatcag acgcaggcgc gctgtggtgt ctttctcatc tttcagaatc agattgatgc
    21781 gagtaatcag gctatcttcg ataaactcaa tcaggctatc gaacatgcgg gtcttactgg
    21841 ggaagtggcg atacagtgcc gcttcggaaa cgccgacaga ggcggccagt tttgccgtcg
    21901 tgatacgttg gcttccatcg ctggattcca gcatcagcgc cagagactga agtatttcct
    21961 cgcgacggtt ccttttcgca gtttgttttt ctgccatgtt acaaaatacc cctgaaaata
    22021 agcacttgcc aggcggcacc cacgctatga ccgcaaacga aatgtttacg gctatgttat
    22081 gacgttattc ggatgcgtat gtgttactga cgaccagagt gaccaaagcc gccttcaccg
    22141 cggtcggtgg cgtcgaaatc ttccaccaga ttaaattcag cctgtactac cggaacaaaa
    22201 atcatctggg cgatacgttc gccaggttga atggtgaagc tgtcctgacc acggttccac
    22261 acggaaatca tcaactggcc ctgatagtcg gaatcgatca atcctaccag gttaccaagc
    22321 acgataccgt gcttatgtcc caatccggag cgcggcagca tcattgccgc cagtgaagga
    22381 tcggcaatat gaatcgccag cccggtcgga accagcgtag tgtcacccgg agccagttct
    22441 acggcgtcgt cgagacaggc acgcaggtca agtccggcag agccagaggt ggcataagtc
    22501 gggagcggaa attccttccc aacgcgcggg tccagaatct taacgtcgat ttttttcatc
    22561 ataacgggtc acgatctcgt cgagtaataa ttggccaagg agctctttgc gctcaagcgg
    22621 taagacttta tctccgtcct gccagaaaag gtgtaatgcg ttgttgtcgc tgttaaatcc
    22681 ttgagttggc tgggaaacat cgttcgcgca gatcagatca aggtttttac ggatacgttt
    22741 ttgccgggcg tattcttcca cattatttgt ttcggcggca aatccaacga cgtagggtcg
    22801 atggtctttt agtgcggcaa cgcctgcgac gatatcgggg tttttaacca tttttattgt
    22861 taattcatca ccctgcgtgg cctgcttttt gattttctct ggggccacgg tagctgcgcg
    22921 ataatccgcc acggcggcgc agccgataaa aatattttgc tgctgtacag aagcattcac
    22981 ggcggcttcc atttccagcg cggtcatcac atcaacacgt ttaacaaacg gtggcgtcgg
    23041 tagtgaaacc ggacctgata ccagcgtgac gttcgcgcca cgacgggcag cggcggcggc
    23101 gatagcaaaa cccatcttgc cggagctgtg attagagata taacgcacgg gatcgagcgg
    23161 ttcacgcgtc gggccggcgg taatcataat gttcagatgt ttcaggtcgt tgacgggcga
    23221 aaaatgcgct accgccatat ccacaatggt taacggatcg agcattcgcc caggaccgat
    23281 atcaccacaa gcctgactgc cactgtctgg cccccagatg agcaaaccac gggaagcaag
    23341 cacctctaaa ttatgctgcg tggcagcggc acggtacatc tgctggttca tggcggggag
    23401 cacggctaca ggcgcaggtg tagccagaca aatcgtcgat accaggtcat tcgccattcc
    23461 ggcagcaaca cgggcaatca aatctgccgt ggcaggggcg agaatcacta aatcagccca
    23521 tttacccagc tcaatatggc ccatagcggc ttcggctgcc gggtccagca aactgtcgga
    23581 aacgggataa ccagaaaccg cctgcaagct aagtggggtg ataaaggctt ttgccgcttc
    23641 ggtcatggct acgcggacgt cggccccgcg atcgcgcaaa cgacgcacca gttcaggggt
    23701 tttataggca gcaataccgc cgctaacgcc gagaacgatt tttttaccgg ccaggctcat
    23761 gatgattttt cctgttgggt gacaccagaa gttggcgatt ttatcacaat acttttgttg
    23821 tcgtgccttc acctgagatt cactttgcga ggcgctttcc aggattgaaa actggccgtc
    23881 gatttaacgg aacggctatg acaggatgcg agcaccacaa aggaggtgaa ggtgaaaaac
    23941 aatgcacagc tgttgatgcc gcgcgaaaaa atgctgaagt ttggtattag cgccttaact
    24001 gatgtcgagc tgctggcgct atttctgcgt accggaacgc gcggtaaaga tgtattaacc
    24061 ctggcaaaag agatgctgga gaatttcggc tctctttatg gcttgttaac ctctgaatat
    24121 gaacaattta gtggtgttca tggaattggc gtagcgaaat ttgcccagtt aaaggggatt
    24181 gctgaactgg cgcgacgtta ctacaacgtg cggatgcgtg aagaaagccc tttactcagc
    24241 ccggagatga cgcgggaatt tttacaaagc cagctcacgg gtgaggagcg ggagatcttt
    24301 atggtgatct ttctcgactc ccaacaccgg gttataacgc atagccgtct tttttccggc
    24361 acgctaaacc atgttgaagt ccatcctcgg gaaattatcc gcgaagcgat aaaaataaac
    24421 gcctcggcgc tgatccttgc acataatcac ccttcgggtt gtgctgaacc cagtaaagcg
    24481 gataaactca ttactgaacg gataataaag agttgtcagt tcatggattt acgcgtgctc
    24541 gaccatatcg tgattgggcg tggagagtat gtttcttttg ccgaacgcgg ctggatttaa
    24601 cccgctatgc gcgatccttc gggatctttg tctgttcggg acttgagcac atcgctgagt
    24661 cagcgtatac tacgccacct ttgagaatct cgggtttggc atttgggcct ggcaatcgag
    24721 agttcacata gaactgcgat gaccgggctg taaagcctga cgaggcgcca ataccccata
    24781 cgaagctcga gctaatttga tttttggaga atagacatgt cccgagtctg ccaagttact
    24841 ggcaagcgtc cggtgaccgg taacaaccgt tcccacgcac tgaacgcgac taaacgccgt
    24901 ttcctgccga acctgcactc tcaccgtttc tgggttgaga gcgagaagcg ttttgtcacc
    24961 ctgcgcgtat ctgctaaagg tatgcgtgta atcgataaaa aaggcatcga tacagttctg
    25021 gctgaactgc gtgcccgtgg cgaaaagtac taagtactta gaggaaataa atcatggcta
    25081 aaggtattcg tgagaaaatc aagctggttt cttctgctgg tactggtcac ttctatacca
    25141 ctacgaagaa caaacgtact aagccggaaa aactggaact gaaaaaattc gatccagttg
    25201 ttcgccagca cgtgatctac aaagaagcga aaatcaaata attctcgctt tgatgtaaca
    25261 aaaaacctcg ctccggcggg gttttttgtt atctgcttgc ccccatattg actgcatctg
    25321 ttcattcctg gagatgctat gcctgaatta cccgaagttg aaaccagccg ccgcggcata
    25381 gaaccgcatc tcgttggtgc aaccattctt catgcagtgg tgcgcaacgg acgcttgcgc
    25441 tggccggttt cagaagagat ctaccgttta agcgaccaac cagtgcttag cgtgcagcgg
    25501 cgggctaaat atctgctgct ggagctgcct gagggctgga ttatcattca tttagggatg
    25561 tctggcagcc tgcgcatcct tccagaagaa cttccccctg aaaagcatga ccatgtggat
    25621 ttggtgatga gcaacggcaa agtgctgcgc tacaccgatc cgcgccgctt tggtgcctgg
    25681 ctgtggacca aagagctgga agggcataat gtgctggccc atcttggacc ggagccgctt
    25741 agcgacgatt tcaatggtga gtatctgcat cagaagtgcg cgaagaaaaa aacggcgatt
    25801 aaaccgtggc tgatggataa caagctggtg gtaggggtag ggaatatcta tgccagcgaa
    25861 tcactgtttg cggcggggat ccatccggat cggctggcgt catcactgtc gctggcagag
    25921 tgtgaattgt tagctcgggt gattaaagcg gtgttgctgc gttcgattga gcagggtggt
    25981 acaacgctga aagattttct gcaaagtgat ggtaaaccgg gctatttcgc tcaggaattg
    26041 caggtttacg ggcgaaaagg tgagccgtgt cgggtgtgcg gtacgccgat tgtggcgact
    26101 aaacatgcgc agcgggcaac gttttattgt cggcagtgcc agaagtaatt catgcgcgcc
    26161 ggatggcata ccatccggca taaacgctac gctaacttcg ccatcagcgc ctgatggaca
    26221 ttctccggca ggaaatgggt gacatcgccc tgatggcgcg ccacctcttt caccaacgat
    26281 gaagagataa acgaccactc tttcgacggc atcagaaaca cactttccag ttccggcatt
    26341 aagtggcgat tcatatgcgc cagctgcatt tcatattcaa aatctgccac cgcacgcagg
    26401 ccacgaatca gcaccgtagc gtgttgatta cgggcgaagt tcgccatcaa atcactaaac
    26461 ccgaccactt ccacgttccc cagatgcgcg gttgcctgct gtgccagtgc cacacgctct
    26521 tccagggtaa acatcggttt tttactgggg ctggcggcaa tcgccagaat aacgtgatcg
    26581 aacatctgcg tggcgcgcgt cacgatatcg atatgaccat tggtaatggg atcgaaagta
    26641 cccggataaa tcgcccgttt ttgcataaca acctcaatgc gttttcggtg gcaggtaagg
    26701 ttccagcagt tgaagcagac gctgtagcgc gccctggttt tgatacagta cttcaacggc
    26761 atgacggcca tagaaactac ggtaatcggc gtcggtgagt aaagaggaaa cctcttttgc
    26821 aagcgtagtg gcatcggtaa cggtaatcag cccgcttgcc tgctccagcc gcgcgcaaat
    26881 gtctttaaag ttaaaagtat gcggccccat caataccgga attgcgtgtg cggcagcttc
    26941 cagcggatta tgcccaccac gttcaaccag tgaaccgcca acaaaggcga gatcggcaat
    27001 gccgtacagt aacatcaact cgcccatcgt atcgccaacc acaacctgcg tgctggtgga
    27061 ggggacttcc cctgaagagc gtgtgatata gcttagtcca gcctggcgga caaggttaat
    27121 cgcatccggg aagcgttctg gatgacgggg taccaggatg agcaataaat tcgggaattg
    27181 ctgtaataaa gcctgatgtg cggcaatgac cacgctctct tcaccttcgt gagtgctggt
    27241 ggcaatccat accgggcggt gtggtgccca ctggcggcgc agcgtcacgg ctttagcagc
    27301 caactgcggc gttacagaaa tatcgaattt caggctaccg gtaacggtta cctggttatt
    27361 tttcgcgccc agcgccacaa aacgtgcacc atcttcttca ttttgggcgg caatcagcgt
    27421 aatacgacgc agcaagcgac ggacgaattt acccagtttg gcataacctg cggccgagcg
    27481 ggcagagagt cgcgcgttag cgatcaccag cggaatttta cgtttatgta gcgccgcaat
    27541 caggttaggc catagttcgg tttccataat caacaccagt ttagggtcga ctttattcag
    27601 gaaacggttg agtgcatcgg gcagatcata cggcagataa acgtgctgaa catccttccc
    27661 gaaagccgat tgtacgcgct ccgaaccggt tggcgtcatg gttgttacgg taataggtaa
    27721 atcaggataa cgatgacgca gcgcgcgcac caacgggatc gccgccagag tttcaccgac
    27781 ggagacggag tgcagcataa tgccgcctgg ttttagcgga tggcggtaaa aaccgtaacg
    27841 ttcaccccag cgttttcgat aggccggagc cttacgtccg cgcacccaga gccgtatcca
    27901 gatcagcggc tgaataaggt agagaagggc ggtgtaaagc aattcgagca tagtaaatag
    27961 ctgacttatg gatgtgctgg ggattctatg tatttagctg tggctttacc attacttttc
    28021 ccgtttttga cttaaatagc aatttacccg ccaattcttg ctgaacatct atactttttt
    28081 tgtattttta tgcgattcat tgatttctgc tgtacgtgca ggaaggaatc gcagagcaac
    28141 agtgaaagaa tatggttgct aatgatttta tttgattgat ttctttattg aaaatcaaca
    28201 atgtgcttaa aaatcattcg ggtcggattg ccgcgaaaga agtgatacac tagcgcgtca
    28261 atatcagtgc ggtatcagta ttcaggtagc tgttgagcct ggggcggtag cgtgcttttt
    28321 ctgcttcacc ttaatcggat aatctcaaca aaaagagtta cttgtggata agccatttcg
    28381 aagaattttg ctcattaaga tgcgttttca tggggatatg ttattaacta ctcccgtcat
    28441 tagttcgctg aaaaaaaatt accctgacgc aaaaatcgat gtgctgcttt atcaggacac
    28501 catcccgatc ctgtctgaaa atccagagat taacgcgctc tacggcataa aaaataaaaa
    28561 agcaaaagcc tcagaaaaaa ttgccaactt ttttcatctc atcaaggtat tacgtgccaa
    28621 taagtatgac cttatcgtca atcttaccga tcaatggatg gttgctatac tggttcgctt
    28681 attaaatgcc cgtgtgaaaa tttcccagga ttatcatcat cggcagtctg ctttttggcg
    28741 taaaagtttc acccatttgg tgccgttgca gggtggaaat gtggtggaaa gtaacttatc
    28801 cgtgctgaca ccattgggac ttgattcgtt ggtgaagcag acaaccatga gttacccgcc
    28861 tgcaagctgg aaacgtatgc gtcgcgaact tgatcacgct ggtgttggac aaaattatgt
    28921 ggttatccaa cctacggcgc ggcaaatctt caaatgctgg gacaacgcca agttttccgc
    28981 tgtgattgat gccttacatg ctcgtggtta tgaagtcgtt ctgacgtccg gcccagataa
    29041 agacgatctg gcctgcgtca atgaaattgc gcaaggatgc cagacgccac cagtaacggc
    29101 gctggctgga aaggtgacct tcccggaact tggtgcgtta atcgatcatg cgcagctgtt
    29161 tattggcgtt gattccgcac cggcgcatat tgccgctgca gttaatacgc cgctgatatc
    29221 gctgtttggt gcgacagacc atattttctg gcgtccttgg tcaaataaca tgattcaatt
    29281 ctgggcggga gattaccggg aaatgccaac gcgcgatcag cgtgaccgaa atgagatgta
    29341 tctttcggtt attccggcgg cagatgtcat tgctgctgtc gataaattac tgccctcctc
    29401 cacgacaggt acgtcgttat gatcgttgct ttttgtttat ataaatattt tccctttggc
    29461 ggtttgcagc gcgattttat gcgtattgct cagacagtcg ccgcccgagg tcatcatgtt
    29521 cgggtttata cccagtcgtg ggaaggcgaa tgccctgatg tatttgaact gatcaaagtg
    29581 ccggttaaat cgcataccaa tcacgggcgc aatgcggagt attttgcctg ggtgcaaaaa
    29641 catttacgcg aacatcccgt cgataaagtc gttggattca acaaaatgcc ggggctggac
    29701 gtttattatg ccgctgatgt ttgttatgcc gagaaagtcg cgcaggaaaa aggctttttc
    29761 tatcacctga cgtcacgtta tcgccattat gccgcctttg agcgggcaac cttcgaacag
    29821 ggcaagccga cacagctgct gatgctgaca gataagcaaa tcgccgattt ccagaaacat
    29881 tatcagactg aagcggagcg ttttcatatt ctaccaccgg ggatttatcc tgatcgtaaa
    29941 tatagccagc agccagctaa tagccgtgaa atcttccgta agaagaatgg aataaccgaa
    30001 caacaatatt tattgttgca ggtcggttca gacttcacgc gtaaaggtgt cgatcgttcc
    30061 attgaagcac ttgcttcgtt accggattcg ctgcgccaca acacattgct atatgttgtt
    30121 ggacaggata aaccgcgaaa atttgaggcg ctggcagaaa aacgcggcgt gcgcagtaat
    30181 gttcacttct tctcggggcg caacgatgtc tcggaattaa tggcggcggc ggatttatta
    30241 ctgcatcctg cctaccagga agcagcggga attgtgctac tggaagcgat tactgcagga
    30301 ttaccggtac taacaacagc cgtttgtggc tatgcgcatt atattgtcga cgccaattgc
    30361 ggcgaggcta ttgctgagcc attccgccag gaaacattga atgagatttt acgcaaagcg
    30421 ttaacgcaat cttcattgcg ccaggcctgg gcggaaaatg cgcgacatta tgctgataca
    30481 caagatttat acagtctgcc agagaaagcg gcggatatca taacgggtgg tctggatggt
    30541 tgaacttaaa gagccgtttg ccacgttatg gcgcggtaaa gatccttttg aggaagttaa
    30601 aaccttgcag ggtgaggttt ttcgtgaact ggaaactcgc cgcactctgc gatttgaaat
    30661 ggcgggcaaa agctattttc tcaaatggca tcgcggcacg accctgaaag agataatcaa
    30721 aaatttactc tcattgcgga tgccagtatt aggcgcagac cgcgaatgga atgcgattca
    30781 tcgactgcgg gatgtcggcg ttgatactat gtatggggtg gcatttggcg aaaaaggcat
    30841 gaatccgctg accagaactt catttattat taccgaagat ctgacaccaa ccataagtct
    30901 tgaagattac tgtgctgact gggcgactaa ccctccagat gttcgcgtaa agcgtatgct
    30961 tattaagcgt gtcgcgacga tggtgcacga tatgcatgct gcgggcatta accaccgtga
    31021 ctgttatatc tgtcatttcc tgctgcactt gcctttttcc ggtaaggaag aggagttaaa
    31081 aatttcggta attgacctgc accgggcgca gcttcgcacg cgcgttccac gtcgttggcg
    31141 ggataaagat cttattgggc tttatttttc ttcgatgaat atcggcctga ctcagcggga
    31201 tatctggcgg tttatgaaag tgtattttgc cgccccgctt aaagacattc tcaagcagga
    31261 acaaggactg ctgtcgcaag cagaagcaaa agccacaaaa atcagggaaa gaacgattcg
    31321 aaaatcgttg taacccgtaa cttatttttg ccaaaatttt ggatac
//
LOCUS       DBFIFI010000037        31287 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.037, whole genome shotgun sequence.
ACCESSION   DBFIFI010000037 DBFIFI010000000
VERSION     DBFIFI010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.037"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(51..1589)
                     /gene="cadC"
                     /locus_tag="N7B39_001596"
     CDS             complement(51..1589)
                     /gene="cadC"
                     /locus_tag="N7B39_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HGU2421602.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSSLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSI
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQETLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
     gene            complement(1849..2008)
                     /locus_tag="N7B39_001597"
                     /pseudo
     CDS             complement(1849..2008)
                     /locus_tag="N7B39_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(2206..2281)
                     /locus_tag="N7B39_001598"
     tRNA            complement(2206..2281)
                     /locus_tag="N7B39_001598"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2246..2248),aa:Phe,seq:gaa)
     gene            complement(2388..2963)
                     /gene="yjdC"
                     /locus_tag="N7B39_001599"
     CDS             complement(2388..2963)
                     /gene="yjdC"
                     /locus_tag="N7B39_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU2421603.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            complement(3000..4697)
                     /gene="dsbD"
                     /locus_tag="N7B39_001600"
     CDS             complement(3000..4697)
                     /gene="dsbD"
                     /locus_tag="N7B39_001600"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HGU2421604.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPVDQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQPT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLTIVFTLLAMSMFGLLTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFIMGTIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAQLRDRQP"
     gene            complement(4673..5011)
                     /gene="cutA"
                     /locus_tag="N7B39_001601"
     CDS             complement(4673..5011)
                     /gene="cutA"
                     /locus_tag="N7B39_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HGU2421605.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            complement(5127..6428)
                     /gene="dcuA"
                     /locus_tag="N7B39_001602"
     CDS             complement(5127..6428)
                     /gene="dcuA"
                     /locus_tag="N7B39_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HGU2421606.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            complement(6546..7982)
                     /gene="aspA"
                     /locus_tag="N7B39_001603"
     CDS             complement(6546..7982)
                     /gene="aspA"
                     /locus_tag="N7B39_001603"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HGU2421607.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            8319..8795
                     /gene="fxsA"
                     /locus_tag="N7B39_001604"
     CDS             8319..8795
                     /gene="fxsA"
                     /locus_tag="N7B39_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HGU2421608.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            complement(8814..10067)
                     /gene="yjeH"
                     /locus_tag="N7B39_001605"
                     /pseudo
     CDS             complement(8814..10067)
                     /gene="yjeH"
                     /locus_tag="N7B39_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
     gene            10343..10636
                     /gene="groS"
                     /locus_tag="N7B39_001606"
     CDS             10343..10636
                     /gene="groS"
                     /locus_tag="N7B39_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HGU2421609.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            10680..12326
                     /gene="groL"
                     /locus_tag="N7B39_001607"
     CDS             10680..12326
                     /gene="groL"
                     /locus_tag="N7B39_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HGU2421610.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            12464..12817
                     /gene="yjeI"
                     /locus_tag="N7B39_001608"
     CDS             12464..12817
                     /gene="yjeI"
                     /locus_tag="N7B39_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HGU2421611.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(13010..13879)
                     /gene="yjeJ"
                     /locus_tag="N7B39_001609"
     CDS             complement(13010..13879)
                     /gene="yjeJ"
                     /locus_tag="N7B39_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HGU2421612.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQQNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLVGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(14114..15142)
                     /gene="epmB"
                     /locus_tag="N7B39_001610"
     CDS             complement(14114..15142)
                     /gene="epmB"
                     /locus_tag="N7B39_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HGU2421613.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RNAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEIDETFRQAMAKLRRAGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            15184..15750
                     /gene="efp"
                     /locus_tag="N7B39_001611"
     CDS             15184..15750
                     /gene="efp"
                     /locus_tag="N7B39_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HGU2421614.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            15802..15927
                     /gene="ecnA"
                     /locus_tag="N7B39_001612"
     CDS             15802..15927
                     /gene="ecnA"
                     /locus_tag="N7B39_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HGU2421615.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            16038..16184
                     /gene="ecnB"
                     /locus_tag="N7B39_001613"
     CDS             16038..16184
                     /gene="ecnB"
                     /locus_tag="N7B39_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HGU2421616.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            16360..16677
                     /gene="sugE"
                     /locus_tag="N7B39_001614"
     CDS             16360..16677
                     /gene="sugE"
                     /locus_tag="N7B39_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HGU2421617.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(16674..17207)
                     /gene="blc"
                     /locus_tag="N7B39_001615"
     CDS             complement(16674..17207)
                     /gene="blc"
                     /locus_tag="N7B39_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HGU2421618.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(17295..18428)
                     /gene="ampC"
                     /locus_tag="N7B39_001616"
     CDS             complement(17295..18428)
                     /gene="ampC"
                     /locus_tag="N7B39_001616"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HGU2421619.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIVNGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            complement(18491..18850)
                     /gene="frdD"
                     /locus_tag="N7B39_001617"
     CDS             complement(18491..18850)
                     /gene="frdD"
                     /locus_tag="N7B39_001617"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HGU2421620.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            complement(18861..19256)
                     /gene="frdC"
                     /locus_tag="N7B39_001618"
     CDS             complement(18861..19256)
                     /gene="frdC"
                     /locus_tag="N7B39_001618"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HGU2421621.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(19267..20001)
                     /gene="frdB"
                     /locus_tag="N7B39_001619"
     CDS             complement(19267..20001)
                     /gene="frdB"
                     /locus_tag="N7B39_001619"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HGU2421622.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(19994..21802)
                     /gene="frdA"
                     /locus_tag="N7B39_001620"
     CDS             complement(19994..21802)
                     /gene="frdA"
                     /locus_tag="N7B39_001620"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HGU2421623.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTKRDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            22127..23104
                     /gene="epmA"
                     /locus_tag="N7B39_001621"
     CDS             22127..23104
                     /gene="epmA"
                     /locus_tag="N7B39_001621"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HGU2421624.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            23323..24825
                     /gene="yjeM"
                     /locus_tag="N7B39_001622"
     CDS             23323..24825
                     /gene="yjeM"
                     /locus_tag="N7B39_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HGU2421625.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(24976..28299)
                     /gene="mscM"
                     /locus_tag="N7B39_001623"
     CDS             complement(24976..28299)
                     /gene="mscM"
                     /locus_tag="N7B39_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HGU2421626.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(28321..29289)
                     /gene="asd"
                     /locus_tag="N7B39_001624"
     CDS             complement(28321..29289)
                     /gene="asd"
                     /locus_tag="N7B39_001624"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HGU2421627.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(29386..30438)
                     /gene="rsgA"
                     /locus_tag="N7B39_001625"
     CDS             complement(29386..30438)
                     /gene="rsgA"
                     /locus_tag="N7B39_001625"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HGU2421628.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            30533..31078
                     /gene="orn"
                     /locus_tag="N7B39_001626"
     CDS             30533..31078
                     /gene="orn"
                     /locus_tag="N7B39_001626"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HGU2421629.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDGWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
BASE COUNT         7632 a         7822 c         8066 g         7767 t
ORIGIN      
        1 caaaaactca acaacaaata ttcccgagca tgaatcaacc cgggggttac ttattctgaa
       61 gcgagaaatt tgtcgagata aggtacaaca taaggaacag aagtctggaa tataccattt
      121 tcaatccagt aaagggtgtt tgcccctggg cgtaaattaa aggcggtgag atatgcatca
      181 gccgcttccc ggttcatccc cttcatttca taaaccttgc caagcaatac ataatttagc
      241 caggacattt caagatcaat gccagtattt atcgcctggt aagactcatc tgttttacct
      301 tttaccagag cactgaccgc ttttatttga tatataatgg acaggttatt caattccggt
      361 agtgtaacaa tgttatctat ttctgtgttc agtgctgcta attgtttttc atctaaagga
      421 tgttgagaat ggcgcacgat atcaactaat gctttttctg ctctcgcgta ggtaaattct
      481 ggggatgatt gaacaatctc acctaataat tcactggcac ggttcaatga tttatcatcg
      541 ccatgcagta aataatcatg tgcctgataa aaattagtta ataacgcacc acgatgaggc
      601 aaaatttgct ggagcgtctc ctgcattcgt tgtggccacg gttggtttaa cgcttttgat
      661 aaactctcca gtaaatcatt ttgaatcgcc agctgattac cgttagtgat gacataacgt
      721 ttatccagca tggttgaacc atctgcattg tctaccaatt ttatcgacat aaagcattgt
      781 tgagcacggt attggcgctg attaacaaac gcaatagata atgttttacc ggaactgctc
      841 ggttcatcaa tgttgtagtt gattttgtca tgcaccataa aggtggagaa ggtgttaagt
      901 gatgtcgcca ccaaatcacc cacgcctatc gcgtaagaga gctgatacgg ggaactccag
      961 ctgttacaac ttttatttac catattaatg tcaatatcgc gtggattgag caaaatacgc
     1021 gatttgctca tcggaagacg tgtatcaaga cttgaaaacg ctaccagtgc tacacagata
     1081 cctaaggaca acaggaaaaa aaaccatacc caaaaggtag tgaatcgttt gcttttaact
     1141 ggggattgtt caggtggcgt tgcggtgttt tgaatgttaa gactgtggga gggagaatct
     1201 gtggcaggaa ccgcctctgg tataggggga ggcgaagata gcattatttc ctctccctct
     1261 tcttcgctgt accagataac cggcaccatt aatttatagc cgcgctttgg tacagtagcg
     1321 atatagacag gactatcttc atcattatct tttaatgact tacgtagttc tgagatactc
     1381 tgcgtcacaa cgtgattggt gacaatactt ctcttccaga cattatcgat aagttcatcc
     1441 ctgctaagta cttcgccact gtgttgagca aagaaaacca gaaggtcgat taatctcggc
     1501 tcaagggtaa gttgacgccc attgcggcta atttggttta tggacggagt aacaagccat
     1561 tcgccaacgc gaactacagg ttgttgcata atagaaactc attcgaaaag ggaatgatgc
     1621 aatgataatt gccacaacct atttttacca tctatagatg ggtttattta catattattg
     1681 gtgaatgcaa gacgttattt ttaccagcca taaacttctt gattacatgg tattacgaaa
     1741 ggattttact gagaaccaga agtaatattc cttaccatca aaattcatca tctttgccaa
     1801 agaaaaatgt tcagaaaata atccatggaa aattgtccgg agcgcttact attttaatgg
     1861 attgttagtc tttgcatgag caagcggact gatacatttc tctttgttct cattcagaaa
     1921 atctcatcag tcggcgttca gaccgatgtt gcgctttatg gatgcgttac gctcctgatg
     1981 acgtcatttg acgttcaaca gcatcacggg gccgcacgac atttcacgtt agttagtgct
     2041 atagctcagg aacaaatttt cccgaattgg gatatgcccg caaattgctg gtgatgtggg
     2101 gagaatcggg ttgagttcgg tagaattgat ttggaggcag aacacttaaa tcgtggcgtc
     2161 ctgaaacgaa aaacggacct ccgtggaggt ccgtttatat gaatttggtg cccggactcg
     2221 gaattgaacc aaggacacgg ggattttcaa tcccctgctc taccgactga gctatccggg
     2281 caacggggcg cattaaaccg taatctgcac atctcgtcaa cctaatttca gtaaaagcga
     2341 ttcaactgct taagattgcg gcaaatcgct tcttttctgt gtttctgtca ggtcagtgca
     2401 ccaccctggc ggcagcgggc gaagcgcagg atatcttccg ccagccgatg tgcggtgtcg
     2461 acatccgcct ggctacgatt caccagcatt cggcttaaac agccttccag caccagttcc
     2521 atctgctttg ctaccatcgc cggatcgtca acttccagcg tggttaacag ttcgtgggtg
     2581 aaatcgtagg ccgcgctttt ttgctgatcg gccagttgat gaatagggtg gccaggatcg
     2641 ggataaaacg tacaggcagc gataaacaga cagcccggat agcggttgtt tttaacgcac
     2701 tccgataacg cctgataacg tgccagcagc ttttgttcgg cggtttgcgt ttcgtccagc
     2761 atcagctgac gacgccagac atctatctgt tggctaagat aacgcagcgc atcgtagagg
     2821 attgcctctt tgtctggcca gaagcggcgt agctcgtcca gtggataatc cacacgttca
     2881 gcaaccatct ccagcgtggt gttggcaatc ccttgtaatt ctaataattt cagggcttct
     2941 cccagtacat cttcacgttg cacgctattt tcctccgtct ttcccactgc aagtgtcgtt
     3001 cacggttggc gatcgcgcaa ttgtgcgctg aaggtttcag catccataaa gcccgtgacg
     3061 cgtgcttgtg gatgctcctg gccttgtccg tcaaaaaaga gaattgtcgg taggccaagg
     3121 acattaagat gctttaacag cgccacatct tgtgcgtcgt tggcagtgac gttggcctga
     3181 agtaataccg tgtctgctaa cgctttttgc acctgcgggt cgctgaaggt gtatttctca
     3241 aactctttac aggcgacgca ccagtcggca taaagatcta acatcaccgg tttgcctttg
     3301 gcttcaacga gcgcctgatt taactcatct accgttttga tttgtgtaaa gttgagatgc
     3361 gtctgagttt gcgcggtatg cgtcgcacca aatgcccaat cctgaagtgg gcgcacgcta
     3421 accaatgccg ctgcaagcag gattatttgc accacgcgca tccagccgcg tttggcctgt
     3481 aggctggtga taaaggccca gccaaagaat gcgacaccca gcgccgacca caagcgtaat
     3541 ccccatacat caccaatcac tcgctccagc aggaagaccg gcagtgcgag gatcacaaaa
     3601 ccaaacgcgg ttttgacttg ttccatccac gggccgcttt tcggcagcaa gcggttacca
     3661 aagacggtaa ttagcatcag cggcaggccc atgcccaacg catagagata aagcgtgccg
     3721 ccgcccagcc acatgttccc gctttgggcg atatacagca gaatcgcgct aagcggtgcg
     3781 gtggtgcatg gtgaacagat cagtccggca atcgtcccca taataaacac tccgccgggt
     3841 gagccgccct gttggcgatt gctcatcagc gtgaggcgcg tttgcagcga ggaggggagt
     3901 tgtagagtaa gcaagccaaa cattgacatc gccagcaagg taaagacgat ggtgaggcca
     3961 atgagcacgt atgggtgctg tagcgccgcc tggaactgta accctgcggc ggcaactacc
     4021 agacccagcg ccgtgtaagt cagtgccatc ccctgcacat aaataaaggt cagcaacaat
     4081 gctctggcag tggagagccg ctgtttacca cccagcacga tgccagaaat cagtgggtac
     4141 attggcagca cgcatggcgt aaaggcgata ccaataccga tcaacaacgc ccagagcgcg
     4201 gaaaagggca attgcgcggt gggctgctct tgctgcggaa cagacacagg ctgtgacgct
     4261 gcgttgttgg cgaccacttc gcttaacgga acggttttgg tttctggcgg ataacagaaa
     4321 ccggcatcag cacagccctg gtaggtgaca gttaacgtcg ctcccgcact cgcctggttg
     4381 atggttacgg gaagcgtcag ccgatcgcgg taaatctcgc ttttgccgta aaactcatct
     4441 tcatgccaga cgccttgcgg cagctgcacg tcggcaattt tcgcgtgttc cggcgtaatg
     4501 cggatctgtt tacggtagag gtagtaaccg tctttgatct gccaggtcag attaaggtca
     4561 tgttggtttt gctgaaaatc aaaagcaaag gcttgatcca cggggacaaa ttgtgaacgt
     4621 cccggcgcgt cgaataatcc ggcaaaaacg gaagtgctgc aaagtagcag gatcagcgta
     4681 aagatgcgtt gagccatgag aggtaatctg tgtctccgtg tgtaacaggt aaaaccagaa
     4741 gttccggggt ttgatatgga tgatgagact tcaggcattc cagcagtgcc tgctggtgag
     4801 atacggtagt ttttaaaatc atctgcactt cgtattcttg ctccagctta ccttcccagt
     4861 aatagagaga ggtagcgccg gggatcaagg tcgcgcaggc cgccagtttt tccgccagca
     4921 ctttggcggc taaatcctgg gctgtcgctt catccggtgc ggtacatagc accacgacag
     4981 acgcggtatt cgaacttttt tcatcaagca taaacacctc gcaagaacag atggaaccgc
     5041 aagagagagg tcactataca acggacgggg aaaggatgtt agtcggcgag aaagaaagcg
     5101 gggcgtgaac gccccgcgat tggtcattac agcatgaagc tacccagcac gaagccgaag
     5161 caaacggcca gggcaacacc cagagtaccc gggatgaaga acggatggtt gaagacgaat
     5221 ttaccgatac gggtagtacc cgtgtcatcc atctgtaccg cagcaaccag cgtcgggtag
     5281 gtcggcagaa tgaacagacc agacaccgca gcgaaagaag caacagcggt cagcggagaa
     5341 acgttcagtg ccagagccat cggcatcagt gcttttgcgg ttgcagcctg agagtacagc
     5401 agagcagaag caaagaagaa gatgacggcc agcagccacg gatgaccctg aatcacttca
     5461 ccagcggtat ctttgatcca gtcgatgttg ttggaaacga aagtatcgcc cagccacgca
     5521 acacccagga tacaaataca ggcgctcata cctgctttga aggtgctgga gttgaggatg
     5581 ttgtcggtat cgactttaca gataacggtg gtcagagttg caacactgag catgatgatc
     5641 aggattgcgt tggtggtgtt catcagcggt ttttcaacca gacccatgct tgggctgttg
     5701 atgattgcat agataaccac gccaactacg cccagcagga acagccagac ggacgttttt
     5761 gcaccggatt tgatttcaat ctgcttttca ccgcgcagtt caaccaggcc ctcttccaga
     5821 cgcttgcgat aaatcggatc gtcagagagt ttggagttga acagcatagt gaccaggaag
     5881 gacatcacca gaaccgccag cagggtggac gggatgacca cggagagcag atggaggtag
     5941 ctgatgccat gaccttccat cacggaagac atgtaaacca ctgccgctga gattggcgat
     6001 gcggtgatcg caatctgcgc ggataccact gcagtagaca gcggacggca aggtttaacg
     6061 ccttgttcct tcgcaacttc agcgataact ggcagtgtcg ccagagagat gttgccagta
     6121 ccagcaaaga tagtcaggaa ataggtcacg atcggtgcga ggatcgtgat gtatttcggg
     6181 ttacggcgca gcagcttttc tgtctgatga accagatagt ccagaccgcc agcaacctgc
     6241 atggcagaaa tagcggcgat aaccgccatg ataatggaaa tgacatcgaa cgggatgtta
     6301 ccgggtttaa cgccaatagc ggcaagaacc agcaccccca atccgcctgc aaaaccaata
     6361 cctattcccc ccaatctggc gcccaagaag atcgccagca aaactatgat gagttctaca
     6421 actagcatat tagccttcct tgttttttaa caagttgata ttagattgtt atttttaagt
     6481 tactgctcac aagaaaaaag gcacgtcatc tgacgtgcct tttttatttg tactaccctg
     6541 tacgattact gttcgctttc atcagtatag cgttttgctt tgtaagccgg gtgcatcaga
     6601 ttctgtacgg agaaaatatc gtcaagttcc gcttcagtca acagaccgcg ttccagaacg
     6661 acttcacgta cactcttacc ggtttcggca cagattttac ccacgatgtc accgttgtgg
     6721 tgaccgatga acgggttcag gtaagtaacg ataccgatag agttgtaaac gtaaccttcg
     6781 cacacttctt tgttagcagt gatgccgtta atgcattttt ccagcaggtt gtagcaagcg
     6841 ttggtcagaa tgtgaacgga ttcgaacata gcctggccaa tgaccggctc cataacgttc
     6901 aactgcagct gacctgcttc tgctgccatg gtaacagtgg tgtcgttacc gatgactttg
     6961 aagcatacct ggttaaccac ttccggaaca accgggttta ctttagctgg catgatggaa
     7021 gagcccgcct gcagttccgg caggttgatc tcgttcaggc cggcacgtgg accagaagag
     7081 agcaagcgca ggtcgttaca gattttggac atcttcacag ccaggcgttt cagcgctccg
     7141 tgaaccataa cataagcgcc gcagtcagag gtcgcttcga tcaggtcttc agccggtacg
     7201 catgggaagc cagtaacttc agccagtttt ttcactgcca gcggagagta ctctttcggc
     7261 gtgttcagac cagtaccgat tgctgttgcg ccaaggttaa cttccagcag cagttcagcg
     7321 gtacgttgga tgtttttcac ttcttctttc agcaggatgc tgaaagcgcg gaattcctga
     7381 ccgagggtca tcggtactgc gtcctgcagc tgggtacgac ccattttcag gatgtcctgg
     7441 aattcgaccg ctttacgttc aaagccttca cgcaattggt taatcgcatc taccagctta
     7501 atcagggaag agtaaactgc gatacggaaa ccggtcgggt aggcgtcgtt agtggactga
     7561 catttgttaa catggtcgtt cgggttcagg tactgatatt cacctttctg gtgacccatc
     7621 agttccagac cgatattggc cagcacttcg ttggtgttca tgtttacgga agtacctgcg
     7681 ccgccctggt agacgtctac cgggaactga tccatgcatt ttccgttgtt caggacttca
     7741 tcacatgcgg caatgatggc attcgctaca cttttaggaa tggtttgcag ctctttgttt
     7801 gccatagctg cggctttttt aaccattacc ataccgcgaa caaattcagg aatatcactg
     7861 attttgttgt tgctgatata gaagttttca atcgctctca gagtgtgaac accatagtag
     7921 gcatcagctg gaacttccct ggtacccaac agatcttctt cgatacgaat gttgtttgac
     7981 atgtgaacct tctttttcaa gctgccaatg atttgcttta aacacacaga atatatgtgg
     8041 tttcgaatgt ttttcgaccg acgattatcc cctgcatcga ccgaatatcc gagatcatat
     8101 gctgcttgag gatttctacc gtaatctgga tcactttaag tgtcggtttt taccccttaa
     8161 ttattaattt gtgaaataga tcaccgcttt gggattacta ccaaaaatag ttgcgcaaat
     8221 atcttgaaat tttgctaatg accacaatat aagctgaacg cgattcgcaa cccattcagg
     8281 tagccgggtt taaccggctg ctattacagg agaaaccttt gcgctggtta ccttttattg
     8341 ccattttcct ttatgtctat attgagattt caatctttat tcaggttgcc catgtattgg
     8401 gggtattgct gactctcgtg ctggttatat ttacgtcagt tatcggtatg tcactggtac
     8461 gtaaccaggg ctttaagaat ttcgtgctga tgcagcaaaa aatggcggcg ggtgaaaacc
     8521 cagcggcgga gatgattaaa agtgtttcgc tgatcattgc cggtttgctg cttttattac
     8581 cgggcttttt caccgacttc ctcggtcttc tgcttttatt gccgccggtg caaaagcatc
     8641 tgacagtgaa gttgatgccg catttgcgct tttctcgcat gccaggcggc ggttttagcg
     8701 ccgggaccgg tggcggtaat acttttgatg gtgagtacca gcgaaaggat gatgaccgcg
     8761 accgccttga tcataaagac gatcgccagg attaatgtcg aaacgccgga tgatgtggtt
     8821 atgccatttt ccggcgtttt tcgttttggc agaaacagcc ataaccccgc cagcatgatc
     8881 agcgcataga gacttttcca gccgatcatt gccagtaaca gaacgcataa cagcccgcca
     8941 accaccgcca gtagtcgata acgaccttgc aataatttac agcctgccag catgcataac
     9001 agataaatca taataaagat gccattggca taaataataa gagcgtccag attgatctct
     9061 aaagcatgaa tcaccaacgt gctcaccacg cagcagccga gcaccgcatt gagggcatta
     9121 ttcgggatat ggcgagaaga gaggcgtgcc aggtagtggt caggattatg ttgcgcctgc
     9181 gaccagacca ggcgggcgaa gctctgtata taaatgttga gactggcaaa gcaggccaga
     9241 tagccaatta cgcaggcaat ccataacgct cctacaccga acagttgtac tacaattttt
     9301 ggaagcgatg ctgccgcggc catttgttca ccataggcgt cgaagtgtaa gacgactacc
     9361 gtacagcccc agtagactaa tcctgccagc agcagaccaa tcatcaaagc acgaggaaaa
     9421 tcacgttctg gatttttaaa ttccgaggca agatgggcaa atgcttccag accgacaaaa
     9481 caccagaaca tcactgataa cgcagcaaat aacccggtaa gttcgatatt tcctggcgcg
     9541 gggaagggga tactcgcagg tttgatatcg cccgcccacc agatagcgac aatcagtgcg
     9601 acgataagtc cggcaataac tgtttgtaga tttgcactgg aactggcacc gcgagtaccg
     9661 atataccaca ccaacgccag cgtaccgagt tctgccaaca acagttgcca gctatgccag
     9721 ccaaacattg cctggccgaa tccggcagca atttgtagcg cggcaggcaa acccacggga
     9781 atgaccgata aaaacagcca gccggtgact cgctcaagcc gcgaaccaaa cgccataccg
     9841 acgaagtgcg cgacgccgcc tgcgctggga tagtggcgac ccagaatcgc aaacacaatc
     9901 gcaatcggga acactaagat aatcaaaatg ggccacgccc acaggctgtt attgcccgct
     9961 accagtgcag ctaacgcagg aacggcaaac acgccagtgc ctaataatga cgtcgatagc
    10021 aggccaatgc cctgggccag ccccagttct tgtttgagtc cactcatggg ttgatgtccg
    10081 attgcgccca aattttgggc aactgcgtag attttcgatg gtagcacaat cagattcgct
    10141 tatgacggcg atgaagaaat tgcgatgaaa tgtgaggtga atcagggttt tcacccgatt
    10201 ttgtgctgat cagaattttt tttctttttc ccccttgaag gggcgaagcc tcatccccat
    10261 ttctctggtc accagccggg aaaccacgta agctccggcg tcacccataa cagatacgga
    10321 ctttctcaaa ggagagttat caatgaatat tcgtccattg catgatcgcg tgatcgtcaa
    10381 gcgtaaagaa gttgaaacta aatctgctgg cggcatcgtt ctgaccggct ctgcagcggc
    10441 taaatccacc cgcggcgaag tgctggctgt cggcaatggc cgtatccttg aaaatggcga
    10501 agtgaagccg ctggatgtga aagttggcga catcgttatt ttcaacgatg gctacggtgt
    10561 gaaatccgag aagatcgaca atgaagaagt gttgatcatg tccgaaagcg acattctggc
    10621 aattgttgaa gcgtaatcct cgcacgacac tgaacatacg aatttaagga ataaagataa
    10681 tggcagctaa agacgtaaaa ttcggtaacg acgctcgtgt gaaaatgctg cgcggcgtaa
    10741 acgtactggc agatgcagtg aaagttaccc tcggtccgaa aggccgtaac gtagttctgg
    10801 ataaatcttt cggtgcaccg accatcacca aagatggtgt ttccgttgct cgtgaaatcg
    10861 aactggaaga caagttcgaa aacatgggtg cgcagatggt gaaagaagtt gcctctaaag
    10921 cgaacgacgc tgcaggcgac ggtaccacca ctgcaaccgt actggctcag gctatcatca
    10981 ctgaaggtct gaaagctgtt gctgcgggca tgaacccgat ggacctgaaa cgtggtatcg
    11041 acaaagctgt taccgctgca gttgaagaac tgaaagcgct gtccgtaccg tgctctgact
    11101 ctaaagcgat tgctcaggtt ggtactatct ccgctaactc cgacgaaacc gtaggtaaac
    11161 tgatcgctga agcgatggac aaagtcggta aagaaggcgt tatcaccgtt gaagacggta
    11221 ccggtctgca ggacgaactg gacgtggttg aaggtatgca gttcgaccgt ggctacctgt
    11281 ctccttactt catcaacaag ccggaaactg gcgcagtaga actggaaagc ccgttcatcc
    11341 tgctggctga caagaaaatc tccaacatcc gcgaaatgct gccggttctg gaagccgttg
    11401 ccaaagcagg caaaccgctg ctgatcatcg ctgaagatgt agaaggcgaa gcgctggcaa
    11461 ctctggttgt taacaccatg cgtggcatcg tgaaagttgc tgcagttaaa gctccgggct
    11521 tcggcgatcg tcgtaaagct atgctgcagg atatcgcaac cctgactggc ggtaccgtaa
    11581 tctctgaaga gatcggtatg gagctggaaa aagcaaccct ggaagacctg ggtcaggcta
    11641 aacgcgttgt gatcaacaaa gacaccacca ccatcatcga tggcgtgggc gaagaagctg
    11701 caatccaggg ccgtgttgct cagatccgtc agcagattga agaagcaact tctgactacg
    11761 accgtgaaaa actgcaggag cgcgtagcga aactggcagg cggcgttgca gttatcaaag
    11821 taggtgctgc taccgaagtt gaaatgaaag agaaaaaagc acgcgttgaa gacgccctgc
    11881 acgcgacccg tgctgcggta gaagaaggcg tggttgctgg tggtggtgtt gcgctgatcc
    11941 gcgtagcgtc taaactggct gacctgcgtg gtcagaacga agaccagaac gtgggtatca
    12001 aagttgcact gcgtgcaatg gaagctccgc tgcgtcagat cgtcctgaac tgcggcgaag
    12061 aaccgtctgt tgttgctaac accgttaaag gcggcgacgg caactacggt tacaacgcag
    12121 caaccgaaga atacggcaac atgatcgaca tgggtatcct ggacccaacc aaagtaaccc
    12181 gttctgctct gcagtacgcg gcttctgtgg ctggcctgat gatcaccacc gaatgcatgg
    12241 ttaccgacct gccgaaaaac gatgcagctg acttaggcgc tgctggcggc atgggtggca
    12301 tgggtggcat gggcggcatg atgtaattgc cctgcacctc gcagaaataa acaaaccccc
    12361 gggcagaaat gtctgggggt ttttcttttg gtcatctttc tagtataaga ttcagacacg
    12421 gacgacgcga gtggcgtcca gctcattgat tatggggaat aacatgcacg taaaatactt
    12481 agcagggatt gtcggtgccg cgctactgat ggcgggttgt agctccagca acgaattgag
    12541 tgctgccggt cagagtgtac gcattgtgga cgagcagcca ggcgcagagt gccagctgat
    12601 tggtactgcg acaggtaagc aaagcaactg gctttccggg caacacggag aagagggcgg
    12661 ttctatgcgc ggcgcagcaa acgatctgcg caaccaggcg gctgcaatgg gcggtaacgt
    12721 gatttatggc atcagtagcc cgtcgcaggg aatgttgtcc agttttgtcc cgacggatag
    12781 ccagattatc ggccaggtat ataagtgccc gaactgatgt gatggtgaca aacgtataat
    12841 tgccagatgc gcttcgctta tcaggcctac ataaggcatt gcaactgatt gaatgcttga
    12901 gaatttgtag gccggataag gcgtttacgc cgcatccggc atgaccaacg atcacgttat
    12961 cagtcatttc aggctggatg gctccagcct cgcattagca aagatgagat tatttcgcct
    13021 gtggtgcagt tttgctggtg gattgaacac gtaagtgatg cagcgcgcgt aagcattgat
    13081 cctgggttag cggttgggcg ttattcgccg ctacggtgcg aacatagacc tgacatggca
    13141 cacccacgag ttttttcagt cgtgggctga aatcaagcat tcggcgagtg atcgcctcta
    13201 tttctttacc cggcgttgct tcacgcgttt tcaccactgc gccgctaaat tgttctttgc
    13261 cactttcatc tttaaagcgg taaagcaccg ccagataatg gtcgaacaag ttatgtttcc
    13321 attccggttg ggaataggtg tcatattcca gattgccatt ttgctggcag tcaacgtcat
    13381 ataacggcag gaaatctaac agtgaggtaa tacgtaatgc cagttcacgc atttccgcat
    13441 tattaattgc atgaatgatt gctcgcgcca gcatttcaat ctgcaactca ttgacgacta
    13501 actcacattt gctaccgtca tgtagcgtta atacgaaggt aagattttcg ccttgattat
    13561 cggtaagttc cagcgtgtta acccgacgat taatatcggc atttttaagc tcatcaacca
    13621 gaatttctgg gatgttttct gccattttct taatgacttt atcgcgagct tgctcatact
    13681 gaaggcgtgc agcagcatcc agtttatgct tttgatgcag acgactttcc agcgcaatca
    13741 gcaggtcgcg cagttccatg acagacataa agaacaacga ttctttattg cgaggttctt
    13801 taattttcag cgccagtgca atgaaattat tactcttgcg aataacaccg gtattaacac
    13861 ccttgatgct tatggccatg taggttctcc ctaaccattt ctcaattaaa taattaattt
    13921 taatttataa gccagataaa tgggcttggt agtaatagtt gttaaaataa cataaatagc
    13981 cgtactcact ctatataaac agaaaagaaa ggttaattat tggtgttggt tatataaaga
    14041 gtaacaacag caatgtatat gaataattaa aggttatgta acgggcaaag attaaacttc
    14101 cctgtttaat cagttactgc tggcgtagct ggagatccag cggcgttttg ctgggttcgc
    14161 cgccaatttc tcgcgccagt ttcggcacca gatatcccga caccagtgtc agcaactcac
    14221 gcataatctg ccgtgcttcg tcatcactca ccataaaatg cgccgcgccc tgtactttat
    14281 cgagcacatg caggtaatac ggcatcacgc ctgcatcgaa caacgcatta ctcaggtttg
    14341 ccagcgtttg tgcgttatcg ttcacaccac gtaacagaac gctctggttc agcaaagtaa
    14401 cgcccgcccg gcgcaactta gccatcgcct gacggaatgt ttcatctatc tcattggcat
    14461 ggttgatgtg attcaccagc aagatttgca gcgtagaatg tgaaaagcgt tcaaccagcg
    14521 catctgtgat gcgtgccggg atcacaatcg gcagacgact gtgaatccgc agacgtttga
    14581 tatgcgggat ggcttccagt tgtgtgagca accagtccag ctcgtgatct ttcgccatca
    14641 gcggatcgcc gccggagaaa atcatctcgt ccagttccgg atgcgcagca acatactcaa
    14701 gtgcagtttg ccagttacgc ttgttgccct gattttcggc ataggggaag tggcgacgga
    14761 agcaatagcg gcaatttacc gcgcagccgc ctttgaccag caaaagcgcc cggttgtggt
    14821 atttatgcaa caaaccaggc actacgctgt gctgttcttc cagcgggtcg gtggagaatc
    14881 cgggcgcgac gacaaactca tcttgcgagg taagtacctg acgcaaaagc ggatcgtccg
    14941 gattgccttt ctccatgcga tcgataaatg agcggggcac acgcagggca aacagctttt
    15001 tggcgttgcg tccggccaac agtttttcgt ccgcgtctat attcaaaaga cgcagaagtt
    15061 catcaggatc ggtcacaaca tcggcaagtt gcgttaacca atcttctctg gatggggtat
    15121 ttagggttac aatatgcgcc attttgtggc ttagctacca attaacaaat ttcagagggc
    15181 cttatggcaa cgtactatag caacgatttt cgtgctggtc ttaaaatcat gttagacggc
    15241 gaaccttacg cggttgaagc gagtgaattc gtaaaaccgg gtaaaggcca ggcatttgct
    15301 cgcgttaaac tgcgtcgtct gctgaccggt actcgcgtag aaaaaacctt caaatctact
    15361 gattccgctg aaggcgctga tgttgtcgat atgaacctga cttacctgta caacgacggt
    15421 gagttctggc acttcatgaa caacgaaact ttcgagcagc tgtctgctga cgcaaaagca
    15481 attggcgaca acgctaaatg gctgctggat caggcagagt gtatcgtaac tctgtggaat
    15541 ggtcagccga tctccgttac tccgccgaac ttcgttgaac tggaaatcgt tgataccgat
    15601 ccgggcctga aaggtgatac cgcaggtact ggcggcaaac cggctaccct gtctactggc
    15661 gctgtggtta aagttccgct gttcgtacaa atcggcgaag tcatcaaagt ggatacccgc
    15721 tctggtgaat acgtctctcg cgtgaagtaa tgcggttgtg gtgcggcctg caggctgcac
    15781 catcacttat tcaggtcaga gatgatgaaa cgccttatcg ttcttgtttt gcttgccagc
    15841 acgctgctca cgggctgtaa caccgctcgc ggtttcggcg aagacatcaa acatctcggc
    15901 aactccatct cccgcgctgc cagctaattt ttcttctctt ccgaaaaatc atcagatttc
    15961 catcattttt ggcgatgttg tctattatta atttgctata ggcaaacata aataacatta
    16021 cctaaaagga agacgttatg gtgaagaaga caattgcagc gatcttttct gttctggtgc
    16081 tttcaacagt attaactgcc tgcaacacca cgcgtggcgt tggtgaagac atttctgatg
    16141 gcggtaacgc gatttctggt gcagcaacga aagcgcagca ataagcaata acggtacgac
    16201 agctgtgtcg tgccgtttgt tttttctgcg atagtcacaa aggtaatagt tgaaattccc
    16261 ccgccacctg gcaaaatatc cgttcaacca tcagctttgc aggacgacct gcaaacgcgt
    16321 cttttcaccg gggacggccc caattctccg gagcctgata tgtcctggat tatcttagtt
    16381 attgctggtc tgctggaagt ggtatgggcc gtaggcctga aatataccca cggctttagt
    16441 cgtttgacgc cgagtgttat taccgtgacg gcgatgattg tcagtatggc gctacttgcc
    16501 tgggcgatga aatcgttacc agtagggacg gcttatgccg tgtggacggg tattggcgca
    16561 gtcggcgcgg ccatcaccgg cattgtgctg ctcggtgagt ccgctaaccc gatgcgtctg
    16621 gcgagtctgg cgttaatcgt attggggatt attggtctga aactcagcac tcactaacta
    16681 ccgggctgct gtacccaaat aaacttactg acatcaaacc cttcccgggt cgcgactgcc
    16741 agcatctcct gtttcacttc gtcagaaatg gttggcgtgc gggagagtat ccacaggtag
    16801 tcgcggtccg gtccgcaaac cagcgcatgg cggtattccc gatcgagtgc aataacgtta
    16861 taaccgccat agaaaggacc aaagaatgac actttcagcg cagcgcgagt tggtgcgccg
    16921 gtaaagtacg ctttcccttc actctgctgc cacattcctc tgtcagggtt atagccttta
    16981 ttaatgacat tcaggccgcc gtcatcacgc aggctgtatg ttgcggtgac tttttccagt
    17041 ccacgttcaa agcggtgatc aaaacgggca atctcatacc aggtaccgag atagcgttta
    17101 gcgtcgaaat tattcactac ggtcacgcca cgcggcggcg taggagaact gcaggcaacg
    17161 accagaaatg cagctgtcgc tgcggcaacg agagggagca ggcgcataaa tgtttcctta
    17221 ctggtttttt ctaagtgtag atgacagcaa ggaaaagcgc agaaaaggtc cgaaaattcg
    17281 gacccgatga aattttactg tagagcgttg agaatctgcc aggcggcggc gactctcgct
    17341 ggattgggat agtttttgtt agccagcatc acgataccca gctctttttc tggaataaac
    17401 gcgacatagc taccaaatcc gccggtcgcc cccgttttat gcacccatga tgctcgtact
    17461 gcaggagttg gaggcgtaat cgcttttacg gggcgtgctg ccagtgcaat tttattgtca
    17521 ctgccgttaa cgatgatgtc aggatttacc ggccagtcca gcatttccca gcccagaccc
    17581 tgatacatat cgccggtttg ccagtagcga gattgtgcca gttgtatccc ttgctgaagt
    17641 gttttgtcgt tgatatcacg gggattcata ttgcttcgta cccagcaggc catatcttca
    17701 atggtcgact tcacaccata agtttcagca tctaacgccc ctggcgaaac atgaactgcc
    17761 ttgccttcgc gatatcccca ggcgtaattc ttttcttctg cgggcggtac attaatccac
    17821 gtatggttga gtttgagtgg ctggaagaca cgagtttgca tcgcctgctc aaaactcaaa
    17881 ccagacggct tcacagccag tgcgccgaac aaaccgatac tggagttggc atacagacgt
    17941 tgtgttcctg gagcccatgc aggctgccag ttttgataga agcgcagcaa gtcgcttgag
    18001 gatttcactt catccggcac ctgcaatggc aggccgccag cagtgtaggt tgcgagatgt
    18061 aatagtgtga tcccattcca ctgtttagcg gtaagttcag gccagtattt tgttgcggga
    18121 tcgcttaact tgatttcccc tcgagcaata gcgtcgccac caagcacgcc cgtaaatgtt
    18181 ttgctgaccg aacctaactc aaacaacgtt tgctgtgtga cgggctgctt tttggcgatg
    18241 tccgcatacc cccaggtaaa gtaataaggt ttaccctgat aaattaccgc caccgccata
    18301 ccggggatct tttgttgctc tataagcggg gtaattgtgc gatgcacaat atcgttgatt
    18361 tgttgagggg cagcaaatgt ggagcaagag gcggtaatta ataaggcgca gagcgtcgtt
    18421 ttgaacatag ggtctggttt ccatacaaaa cggcccgcca taggcgggcc ggatttacat
    18481 tggcgatgcg ttagattgta acgacaccaa tcagcgtgac aactgtcagg atagcagcca
    18541 gaccgtagaa aacccatttg cccgcaggta cgtggatttt cagatcgtgc atcgcgtggt
    18601 gcatacggtg taaaccacac cacagcggca gaacgatcat caggaacagg aatacgcgac
    18661 caatgaagct ctgcgcgaac gccagaacgc gctcgtagct cagtgcatca cccggaaaca
    18721 gccccagcgg cagcagaata cccaccagca ggatcatcac cggcgcaata attgcgctcc
    18781 acataccacc ggccccgaag aggccccaga ataccggttc gtcagaacgc tttggatttg
    18841 gattaatcat ctcaggctcc ttaccagtac agggcaacaa acaggattac gatggtggca
    18901 accacagtta ccgcccagag acttttgata attggctctg gtcccatttt ttcgtctttt
    18961 acaatgatat tggccgcttt cggtgccagt tcaaaccagg ttttggtgtg cagcagagct
    19021 gccgccagag tgatcaggtt aatgatcaca ataaccgggt tttgtaaaaa gtcgacgaat
    19081 cccgcccagg cttccgggcc atttttcagg gcaaacagcc cgaaaatcag ttcaatgctg
    19141 aaccacacag ccggaaccgc cgtgccttcg cgcagcatgt aaaagcgata aaacggcaat
    19201 tttttccacc aggtggacgt cattggccgt acatacggtt tacgtttagt cgtcatgttg
    19261 cactccttag cgtggtttca gggtcgcgat aagaaagtct ttcgaacttt ctactttgcc
    19321 ctgttgaatg gccgcagccg gatcgacgtg tttcgggcag acttcggagc agtagcccac
    19381 gaaagtacag ctccatacgc cgttctggct gttcaactgc gccatacgct ccttcttacc
    19441 gtggtcgcgg ctatcttcgt tataacgatg cgccagcgta atggcagccg gaccgatgaa
    19501 ctctgggttc aggccaaact gcgggcacgc ggcgtagcac agaccacagt tgatgcaacc
    19561 ggagaactgg tgatacttcg ccatctgcgc cggggtctgg atgttagtac cctgatccgc
    19621 ggtgcgggag ttgccgatga tgtacggttt gatcgcttcc agactttcga tgaagtgggt
    19681 catatcgacc accagatcgc gttcaatcgg gaagttagct aacgcttcaa ccttcatacc
    19741 gtcggtgtaa tcacgcagga aggttttaca tgccagtttt ggcacgttgt taaccatcat
    19801 gccgcaggaa ccacaaatcg ccatacggca ggaccagcgg tagctcaggt ccggtgccag
    19861 gttgtctttg atgtagccca gcgcatccag taatgaggta gttgcgtcat aaggcacttc
    19921 atagaatgcg ctatgcggtg cggtatcgac ttccgggtta tagcgcacca cctcaatttt
    19981 caggtttttc atctcagcca ttcgccttct ccttcttatt ggctgcttcc gccttatcgg
    20041 ctgcatccgc ttcgccaccg taaacgcgtt tagctggcgg tagcgtagta atcttcacgt
    20101 cgctgtactc caggcgagtc gtgccatcag catcgcggaa ggcgagggtg tgtttgagga
    20161 agttgacgtc gtcacgcttg gtgcaacctt cgtccagacg ctggtgtgcg ccgcgggact
    20221 ctttacgtgc cattgcggag tgcgccatac attcagcaac gttcagaccg tggcccagtt
    20281 caatggtgta gagcaggtcg gtgttgaaca cgctggaagt gtcggtgatg cgcacgcgct
    20341 tgaagcgttc ctgcagctct gccagcttgt cgatggtttt ctgcatcagt tccggcgtac
    20401 ggtagatacc gcaaccttct tccatagcca ggcccatttc gtcgcggatc ttcgcccagt
    20461 tttcgccgcc atcctggtta accagatctt tcagacgttg ttcaacgcca gctgcctgcg
    20521 cttcaattgc cgcttcgttg ccattaccgg cagttgctgc acgctctgtc gcttgttcac
    20581 cggccagacg gccgaagacc accagttccg ccagggagtt ggagcccaga cggtttgcac
    20641 cgtgcagacc aacagaggaa cattcaccca cggcgaacag acctttaatg cgggtttcac
    20701 agttctgatc ggtttcgata ccgcccatgg tgtagtgtgc ggtcggacgt accggaatcg
    20761 gttctttaac cggatcgacg ccaacgtacg ctttcgccag ttcgcagatg aacggcagac
    20821 gttcatgcag ttttttctcg ccgaggtgac gcaggtcgag ataaaccaca tcaccacgcg
    20881 gcgtggagat ggtgttgcct ttacgccatt cgtgccagaa ggcctgagaa actttgtcgc
    20941 gtggacccag ttccatatat ttgtttttcg gctcgcccag cggagtttcc gggcccatgc
    21001 cgtaatcttg cagataacgg tagccatttt tgttgaccag aataccgcct tcaccgcggc
    21061 aaccttcggt catcaggata ccggaacctg gcagaccggt tggatgatac tgaacgaatt
    21121 ccatgtcacg cagcggaacg ccgtggctta gcgccatacc cataccgtca ccggtaacga
    21181 tgccgccgtt ggtgttgtaa cgataaacgc gacccgcacc gccagtagcc ataacgaccg
    21241 cgttagcacg gatctgcacc agcgtgcctt ccatcatgtt cattgctacc aggccgcgaa
    21301 catgaccatc atcaaccaga atatccagca cgaaatgttc gtcaaaacgc tggatctgcg
    21361 ggaattgcag agaggtctgg aacagcgtgt gcagcatatg gaagccggtc ttatcggcgg
    21421 cgaaccaggt acgctcgatt ttcatgccgc cgaagcgacg tacgttgacg ctaccatccg
    21481 ggcgacggct ccatgggcac ccccacagtt ccagttgggt catttcggtt gggcagtggt
    21541 ggacgaaata atccacgaca tcctgctcac acaaccagtc gccacccgct actgtatcgt
    21601 gaaagtgata ttcgaagctg tcatgatcct gcgcgacagc ggcggagccc ccttctgcag
    21661 caacggtatg gctacgcatc gggtatactt ttgagattag tgcgattttt gcatttggat
    21721 ttgcctgcgc ggcagcaatt gcagcacgta atcccgcgcc accggcgcct acaatggcaa
    21781 gatcggcttg aaaggtttgc acgacattcc tccagattgt ttttatccca cagccccgta
    21841 cttcagggta agtgcctgaa agttacggtc tgcgaacgct attccactgc tcctttatag
    21901 gtacaacagt atagtctgat ggataagtct gaaatttgac gagatcgatt tttttagtgc
    21961 gcaagagggc taaattatca ctgaagatga ttaatttaat tactaaacca tcagatcgtg
    22021 ctttttttag tcactgccca ccgttgtttt attcctgcgt aaaatttgtc tcgccgtcgc
    22081 gttgcgagta gacttcgtgc ccttgtcaaa aactggagat ttaactatga gcgaaacggc
    22141 atcctggcag ccgagcgcat ccattcctaa cttattaaaa cgcgcggcga ttatggcgga
    22201 gatccgtcgt ttctttgccg atcgtggagt gctggaggtg gagacgcctt gtatgagcca
    22261 ggcgacggta accgatattc atttggtccc gtttgagaca cgtttcgttg gccccgggca
    22321 ttcgcagggg atgaatctct ggttaatgac cagcccggaa taccatatga aacgcctgct
    22381 ggttgccggt tgcgggccgg tattccagct gtgccgcagt ttccgtaatg aagagatggg
    22441 gcgttatcac aaccctgagt tcactatgct tgagtggtat cgaccgcact atgatatgta
    22501 ccggttgatg aacgaggtgg acgatctctt acaacaggtg ctggactgcc cggcagcaga
    22561 aagcctttct tatcaacaag ctttcttgcg ttatctggaa attgacccgc tctctgccga
    22621 caaaacgcaa ctgcgggaag tggcagcgaa actggatttg agcaatgttg ctgataccga
    22681 agaagaccgc gacacgttgc tacaattgct gtttaccttt ggcgtagaac caaatattgg
    22741 taaagaaaaa ccgacctttg tgtaccactt tccagccagc caggcatcac tggcgcaaat
    22801 cagtaccgaa gatcatcggg tcgctgaacg ctttgaggtt tattataaag gtattgagct
    22861 ggcgaatggt ttccatgaat tgacggatgc ccgtgagcag caacaacgct ttgaacaaga
    22921 taaccgtaag cgcgcggcgc gcggtttgcc gcagcacccc attgatcaga atctgattga
    22981 agccttgaaa gtcggtatgc ctgactgttc cggcgtggca ttaggtgttg atcgtctggt
    23041 gatgttggcg ctgggcgcgg agacactggc tgaagtcatc gcctttagcg ttgaccgggc
    23101 ataattctga aaaattactg aattaacagc gaagaatggc gtgataagcg ccattctgta
    23161 gcatatgttt tcatttttcc gctaaaggcc acaatttaag actggatatt tgatatcatc
    23221 caggtatcaa ttctgtattg gtttttacgc tgcccgctca atgttgggca gtgcagttca
    23281 gtgggcgcgt tttatacacg cgctgaaatg aaggatggtt tcatgcctca cacgataaaa
    23341 aagatgagtc tgataggact catattgatg atctttactt ccgtatttgg atttgccaat
    23401 agcccatcgg cttattactt aatgggttat agtgcgattc ccttttatat attttctgca
    23461 ttgttattct ttattccatt cgccttaatg atggctgaaa tgggagctgc ttatcgcaaa
    23521 gaagaaggcg gtatctattc ctggatgaat aatagtgtcg gaccacgttt tgccttcatt
    23581 ggtacgttta tgtggttttc ctcttatatc atctggatgg tgagcacctc cgcgaaagtt
    23641 tgggtaccgt tctcaacatt cctctatggt agcgacatga cccagcactg gcgtattgcc
    23701 ggactggagc ctacgcaggt ggttggtctg ctggcagtgg catggatgat tctggtcacc
    23761 gtcgttgctt caaaggggat taataaaatt gcccgcatta ctgcggtggg cggtattgca
    23821 gtaatgtgtc tgaatttagt attgctgtta gtaagcatta ctattttgtt attaaatggt
    23881 gggcatttcg cgcaggatat taatttcctt gcatcaccga acccaggtta tcagtccggt
    23941 ctggcaatgc tatcgtttgt ggtatttgct atttttgcct atggcggaat tgaagcggtt
    24001 ggtggtctgg tcgataaaac ggaaaatcca gaaaagaact ttgccaaagg tattgttttt
    24061 gccgctattg ttatttcaat cggttattcg ctggcaatat ttttatgggg cgtcagcaca
    24121 aactggcagc aggtattaag taatggttcc gttaacctcg gcaatattac ctatgtgctg
    24181 atgaagagcc tcggggtgac gctgggtaac gcactgcatt tgtcacctga agcgtcattg
    24241 tcgctgggcg tatggtttgc gcgtattacc ggactttcga tgttcctcgc ttataccggt
    24301 gcgttcttta cgctttgcta ttcaccgctg aaagccatca tccaggggac gccgaaagcg
    24361 ttgtggccgg aaccgatgac gcgcctgaat gcgatgggga tgccgtctat cgctatgtgg
    24421 atgcagtgcg ggttggttac tgtcttcatc ctgctggttt cgtttggtgg cggtaccgca
    24481 tcggcgttct ttaacaagct gacgctgatg gcgaacgtgt ctatgacgct tccttacctg
    24541 ttcctcgcgc tggctttccc attctttaaa gcacgtcagg atctcgacag accatttgtg
    24601 attttcaaaa cgcgtatgtc ggcaatgatt gcgacggtgg ttgtcgttct ggtggtgaca
    24661 tttgcgaacg tcttcaccat tattcagcct gtggttgaag ccggagactg ggacagcaca
    24721 ttgtggatga ttggcggccc tgtcttcttc tcgctgttag cgatggcgat ttaccagaac
    24781 tattgcagtc gcatggcgaa taaacctgag ttagctctcg actgatcata tgaaatcctg
    24841 caactcatcg tctctccggc gatgagttgt caggaagtaa tgcgtatgga tgatactctc
    24901 cagggccggt gataactgaa gatgatgtta agctgctaaa tgagccacgt tgacatcatt
    24961 tggcagcttc atgcattaca aacttcccgc ctgacgacct ttgcccgcag acgtcagcgt
    25021 tctccccgtt tgtttaccgt tgaggctttc cagacgcatc tggaagggcg ggaatggcat
    25081 atcgataccg tgggcatgga agccagccag aatcagctgg tggatctcat ggcgtagcgg
    25141 catacggtga cccatctcag cggcgtaaat acgcagctcg aaaatctgaa tcccctgttg
    25201 cagatccacc aggaagactt ccggtgccgg gttgtcgatc accagcgagc agcgacgcgc
    25261 tgcggtgagc aggatttccg tcacttcttc actgttggca tcggcagggg ccggaatcgt
    25321 caacaccacg cgcgtgaccg agtcagagag cgaccagttg ataaactgct cggtaataaa
    25381 cgccttgttc ggcacgatta tctctttacg gtcccagtcg ctgatggtgg ttgcgcgggt
    25441 attaattttc gtcacgctac cagtaagatc gcgaattgtc accgtatcgc caatgcgaat
    25501 cggtttttcg aacaggatga tcaggccaga gataaagttg gcgaaaattt cctgcaaacc
    25561 aaaaccgaga ccaacaccga gcgcggcgac cagccactgc aatttcgacc actcaatacc
    25621 aatcatcgag aagccgacca gcccgccaat cagcatcagc agatatttgg tgatggtggt
    25681 gatggcgtag ccggtacccg gcgttaaatc caggtgctgc aaaatcgcca gttccagcag
    25741 cgcgggcaga ttgcgcacca gctgcgtggt gatgataaac accagaatgg caatcagcac
    25801 cgcaccgagg gtaatcggct ccagactttc tacgccctgt accgtggagg tgacatccca
    25861 cagcgaaata ttttcgagga agccgaaagc ggaatggatt tctgaccaca gcacaatgac
    25921 cgaaagcagg gcgatcaaca tcaaaattga gcgcactagc cgcaaggact gcgcactgat
    25981 ggcatcgaga tcgacttcgc tttcatcgac ttcaattgcc ccttccgggc tactgtgatg
    26041 atgtgcttcc tcttcgccac gcgcacgttg cgctaacatc tctgcccggc gatgcttcgc
    26101 ccggtcaaac gccagcctgc gtcgttggat cagcatccag cggcggataa cgtgataaac
    26161 caccagtagc aggaaccaga tggcaaccga ggtttcaagc ctcgctaaca gcgcctgtgc
    26221 cgttgccaga taacccaccg ccgacgccag aatggcaacc agtggcgcgc caatcatcat
    26281 gttccacaac atatggttgg taatattgtc gccgctgcct tctttgttga gatacagcgg
    26341 gatcccggcc tttttcaggc tgagggtgac caccgccagc gcaccgcaaa tgaggataaa
    26401 gcaaagccgt cccagcgaac cggaaaattc gcggtcgtcg aggttatcga acatcatcag
    26461 cgccataatc agtggcacaa taagcccgat gctcatcagg tagtagcgca tcccacgaga
    26521 aacacgttcg cgcggccagc caaaatgagc gataaacaag ccgttcgggc gggcaaaggt
    26581 agcgcaaatc atcactaccc acagcagcgg cacggtggct gtgacgccat caccaatcgc
    26641 gaccgccagc ggataaggcc acgcctcgcg caagccgtaa cccagcgtca tccacagcac
    26701 cggcagcggt gacgcgacga gaatcgacca gaaaagagtg cgcaacgtca gccagaagtg
    26761 atcctgagtc actttgccga ctttcgccgc cgaacgttca agaaaacggg tgaaatagcg
    26821 gcgcgagtaa atactgcaac cgaccagaat caacgcgcca aacagcggca aaatcgtctc
    26881 tttgctggtc agcatcatca cactggcttt gcccaactga ctgaaggtgt ccagcgaaat
    26941 gagacgacgc agatcctggg cgatttccag cggccaggcg atggtcatcg ggcgcacgtc
    27001 agaggtccag aacagatagc ggtgggtcgc ttcgttcact tctttcagcg catcttccag
    27061 ttgcccgttg gagactttca gcttggtcag ttccagcagt agcgtatcgc caccctgcaa
    27121 caatgagttc agcaactcac gctgagtgcg tagctgtgct tccagaatac ggttttgctc
    27181 agcggtcagc ggctgaccgt cggcctgatg aatttgccgt agcagcggct gtttattaag
    27241 cagatcctca taacgtaacc gttgcacacg caactgcgcc atttcggtat caagctgttg
    27301 tggtttcggc atttccggca gccgtgccac ctgcgcccgc aacgcttcac cgagcagatt
    27361 ggacgatccc agccattgcg actgttcacg cagcgtattc aacgcctgcc ggacctgtaa
    27421 cgtctggctg gcagcctgac gctgttgcga ggcaacgaga tccatccgct gcgcctgttg
    27481 attcagcgcc gccgatagtt cgcggttaat tttgaattgc gcgacgatat ctttcggcaa
    27541 atcggcactg ttttctgcta gtaattcggt actttccagc gcccgctctg cctcaagctg
    27601 gcgttggctg ttcaattgat tacgcaaggc ctgcaaatac gcatccagtt gctggctctc
    27661 tttttcagcc agctcagagc gcaagcgcgc taattcctgg cggttattgg cagacagctg
    27721 cgccagctcc agttcatcaa cgagcgcctt aagacgtgca gagtcagact gcaacgcgaa
    27781 attttgtgcc tgattgagcg gagtattgcc ggtaagcgtc cccaggcggc gctcgatctc
    27841 atttaactga cggcgggcgt cggtttgctg ttgcggcagt tgattcagcg aatcggcaat
    27901 ctcgcgggcg cgctcctgct cttgctgggc ctgacggctt ttatccagca actgactgct
    27961 gacctggaga atttcctgat tcagcgcgtc ggtagacatc cccggcgaca cgctgcgcgg
    28021 ctcgtcacgc atgttgttta attgtgcgcg cagagtagcg gagagtttcg gataattatc
    28081 aatgacttct tgatattgct tgatgcgctc aagggaacct tttcgttcct caagcgcatt
    28141 taaggcagac tggagtgcct ctacgacttc tggctgcgcg ggtttcgccg cttttgcctg
    28201 ctccagttcc tgggtgattt gtttgctatc gggggccgtc gcggcgtacg ccccccaact
    28261 gaggcaccag gccatcagaa aagtgataat caggcgcacg tcagcgtttc ctttgatgga
    28321 ttagacctgg tcttttttat cgtcaaccaa tgggctggcg tcgtgttctg cttcgatctc
    28381 ttcagcagga agcggggcgg gttcagcgtc tggcgtaaca aaggtttcgg tagatactgc
    28441 cagcggctgg ccaattttcg tgacagacag gctttccagt tgctcaacca gattcacttt
    28501 acccggtgca aacaggttga taacggtaga gccaagttta aagcgaccca tttcctggcc
    28561 tttcagcagc gccacagaac cgtcgttttc cccggcaggc caggtccagc gcttgatgat
    28621 gccttcgcgc ggcggcgtaa tggtgcccgc ccagaccgtc tcaatgctgc ctacaatcgt
    28681 cgccccgacc agaatctgcg ccattgggcc aaattcggta tcgaacaggc agatcacgcg
    28741 ttcgttacgg gcaaacagat tcggcacgtt ctgagccgtg agatggttaa cggagaagag
    28801 atcgcccggc acgtagatca tctcacgcag aataccgttg cacggcatgt gtacgcggtg
    28861 gtagtcacgc ggggagaggt aagtggtcac aaacgtaccg ttgcggaaca ggtccgccat
    28921 cagatagttg cctgccagca gggcttcgag gctgtagttg tggcctttgg cttgcaggat
    28981 tttatcttct tcgattttac ccaactggct gataacgcca tcggcaggca tgaccaggac
    29041 attcggatcg gtatcgattg ggcgtacttc gtcacgcagc ggacggacaa agaattcgtt
    29101 aaaggtgcgg tagctggcgg tgtccggctt ttgcgcctct ttcatgtcga ccttgtagta
    29161 tttaacgaac agatcgataa ccagttttgt cagccatcct gcccgcttgc ttgcgcccca
    29221 acccgccagg cgagtaagcc atagtttcgg cagaatgtac tgtagcgaaa gtttaaatga
    29281 atttaacaag gtagcctcca ggccattgtt ttgtcgttcc tgatccggcc taaatgccgg
    29341 atcctgaaaa aaaggggacg attctaacga cggttagctt aattgtcagt catccgtatc
    29401 agaaaagttt ttacgcgttt ttacctgcgc catgctttcc agaatacggt gatagttttc
    29461 gaaacgggtt tccgcgattt ttccttcctc aaccgcttcc cggatagcgc agcccggatc
    29521 ggtatcgtgt ttgcaatcgc gatatttaca cagacctaaa tagtcatgga attcgacaaa
    29581 gccctgagtg atttgttccg gctccaggtg ccagaggccg aactcacgca caccagggga
    29641 atcaatcaca tcaccgccgt gcgggaagtg atacaggcgt gcggcggtcg tggtgtgctg
    29701 accaagcccc gagttgtcag agacatcgtt ggtcaggatc tctttttgca gccccagcaa
    29761 cgcgttcagc aggctggatt tgccgacgcc agactgcccg gcaaaaatgc tgatgcgccc
    29821 ggtcaacgcc tcttccagcg gttttagccc atcctgagta tggctggaaa ccatcaatac
    29881 gcgataaccg atattgcggt agatatccat ctgttcgttg acgaacgcca tgccttcgtc
    29941 gtccagcagg tctatcttgt tgagcacaat aatcggctca atctgcaagg tttcgcaggc
    30001 caccaggtaa cggtcgataa tattgagcga cagctccggc aaaatggcgg agacaatgac
    30061 aatctggtcg atgttggcgg caataggttt cacgccgtcg tagaaatccg gacgcgtcaa
    30121 cactgaggtg cgctcatgca ccgcttccac gatccctttg acatttacgc cttccgccgc
    30181 cggtttaccc ggacgccaga ctacgcggtc gccggtcacc agcgaacgga tggtacggcg
    30241 aatattgcag cggtgaacgt cgccatcggc ggattccaca tcagcgtgca taccaaagcg
    30301 gctgatgacg ataccttcat caggctcgcc aaacagattg tcgtcgtagt cgggcttctc
    30361 cttagacgtt ttaagacgac gctggtgatt ggcgttcacg cggcgctgct ggcctttgga
    30421 gagtttattt ttactcaatc gtactgactc ctggtcgccc ctgatgggca aaacatctat
    30481 gatacacgca attgtggatc aatatagtca ctgtgaatgg gtggaaaata gcatgagtgc
    30541 caatgaaaac aacctgattt ggatcgatct tgagatgacc ggtctggatc ccgagcgcga
    30601 tcgcattatt gagattgcca cgctggtgac cgatgccaat ctgaatattc tggcagaagg
    30661 gccgaccatt gcagtacatc agtccgatga acagctggcg ctgatggatg gctggaacgt
    30721 gcgcacccat accgccagcg ggctggtgga gcgcgtgaaa gcgagcacga tgggcgatcg
    30781 tgaagctgaa ctggcaacgc tcgaattttt aaaacagtgg gtgcctgcgg gtaaatcgcc
    30841 gatttgcggt aacagcatcg gtcaggatcg tcgcttcctg tttaaataca tgccggagct
    30901 ggaagcctac ttccactacc gttatctcga tgtcagcacc ctgaaagagc tggcgcgccg
    30961 ctggaagccg gaaattcttg atggttttac caaacagggg acgcatcagg cgatggatga
    31021 tatccgtgaa tcggtggcgg agctggctta ctaccgcgag cattttatca agctgtaaaa
    31081 tttttgctgg tcacgtaagc gccggaatcc acttgcggcg gcgtgatttt gacgctaaat
    31141 tgaacgtctt gctgataatt tcagcacttg agataaaaac tcaaaaaaaa atttttaggg
    31201 gggttgcaga gggaaagatt tctcgtataa tgcgcctccc gtaacgacgc agaaatgcga
    31261 aaattacgaa agcaaaatta agtagta
//
LOCUS       DBFIFI010000038        31131 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.038, whole genome shotgun sequence.
ACCESSION   DBFIFI010000038 DBFIFI010000000
VERSION     DBFIFI010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31131
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.038"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..546
                     /locus_tag="N7B39_001627"
     CDS             <1..546
                     /locus_tag="N7B39_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HGU2421630.1"
                     /translation="PIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVGIKETQKSTCT
                     GVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFESEVYILSKD
                     EGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIHPIAMDDGL
                     RFAIREGGRTVGAGVVAKVLS"
     gene            776..1159
                     /gene="secE"
                     /locus_tag="N7B39_001628"
     CDS             776..1159
                     /gene="secE"
                     /locus_tag="N7B39_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HGU2421631.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            1161..1706
                     /gene="nusG"
                     /locus_tag="N7B39_001629"
     CDS             1161..1706
                     /gene="nusG"
                     /locus_tag="N7B39_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HGU2421632.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1865..2293
                     /gene="rplK"
                     /locus_tag="N7B39_001630"
     CDS             1865..2293
                     /gene="rplK"
                     /locus_tag="N7B39_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HGU2421633.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            2297..3001
                     /gene="rplA"
                     /locus_tag="N7B39_001631"
     CDS             2297..3001
                     /gene="rplA"
                     /locus_tag="N7B39_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HGU2421634.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3293..3790
                     /gene="rplJ"
                     /locus_tag="N7B39_001632"
     CDS             3293..3790
                     /gene="rplJ"
                     /locus_tag="N7B39_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HGU2421635.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3857..4222
                     /gene="rplL"
                     /locus_tag="N7B39_001633"
     CDS             3857..4222
                     /gene="rplL"
                     /locus_tag="N7B39_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HGU2421636.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4542..8570
                     /gene="rpoB"
                     /locus_tag="N7B39_001634"
     CDS             4542..8570
                     /gene="rpoB"
                     /locus_tag="N7B39_001634"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HGU2421637.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8647..12870
                     /gene="rpoC"
                     /locus_tag="N7B39_001635"
     CDS             8647..12870
                     /gene="rpoC"
                     /locus_tag="N7B39_001635"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HGU2421638.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            13083..13526
                     /locus_tag="N7B39_001636"
     CDS             13083..13526
                     /locus_tag="N7B39_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase growth adaptation protein"
                     /protein_id="HGU2421639.1"
                     /translation="MKQEVEKWRPFKHPDGDVRDLSFLDAHQVIYVQHHEDKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKLSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVVFRAFSEIFHHCLKLIEK"
     gene            complement(13961..15094)
                     /gene="thiH"
                     /locus_tag="N7B39_001637"
     CDS             complement(13961..15094)
                     /gene="thiH"
                     /locus_tag="N7B39_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HGU2421640.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDSWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDERRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(15091..15861)
                     /gene="thiG"
                     /locus_tag="N7B39_001638"
     CDS             complement(15091..15861)
                     /gene="thiG"
                     /locus_tag="N7B39_001638"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HGU2421641.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLAPQSGPGSRSYFAHATSPLTGFLEASV"
     gene            complement(15863..16063)
                     /gene="thiS"
                     /locus_tag="N7B39_001639"
     CDS             complement(15863..16063)
                     /gene="thiS"
                     /locus_tag="N7B39_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HGU2421642.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLVQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(16047..16802)
                     /gene="thiF"
                     /locus_tag="N7B39_001640"
     CDS             complement(16047..16802)
                     /gene="thiF"
                     /locus_tag="N7B39_001640"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HGU2421643.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSHQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWGQGCYRCLWPENQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16795..17430)
                     /gene="thiE"
                     /locus_tag="N7B39_001641"
     CDS             complement(16795..17430)
                     /gene="thiE"
                     /locus_tag="N7B39_001641"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HGU2421644.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLSDYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17430..19325)
                     /gene="thiC"
                     /locus_tag="N7B39_001642"
     CDS             complement(17430..19325)
                     /gene="thiC"
                     /locus_tag="N7B39_001642"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HGU2421645.1"
                     /translation="MSATKLTRREQRAQAQHFIDTLEGTAFPNSKRIYITGTQPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGIADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19406..19506)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19558..20034)
                     /gene="rsd"
                     /locus_tag="N7B39_001643"
     CDS             complement(19558..20034)
                     /gene="rsd"
                     /locus_tag="N7B39_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HGU2421646.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            20129..20902
                     /gene="nudC"
                     /locus_tag="N7B39_001644"
     CDS             20129..20902
                     /gene="nudC"
                     /locus_tag="N7B39_001644"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HGU2421647.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRYDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKHLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20942..22006
                     /gene="hemE"
                     /locus_tag="N7B39_001645"
     CDS             20942..22006
                     /gene="hemE"
                     /locus_tag="N7B39_001645"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HGU2421648.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVALYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            22016..22687
                     /gene="nfi"
                     /locus_tag="N7B39_001646"
     CDS             22016..22687
                     /gene="nfi"
                     /locus_tag="N7B39_001646"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HGU2421649.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22730..23320
                     /gene="yjaG"
                     /locus_tag="N7B39_001647"
     CDS             22730..23320
                     /gene="yjaG"
                     /locus_tag="N7B39_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HGU2421650.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREADESNIGIIFQQ"
     gene            23507..23779
                     /gene="hupA"
                     /locus_tag="N7B39_001648"
     CDS             23507..23779
                     /gene="hupA"
                     /locus_tag="N7B39_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HGU2421651.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23792..24487
                     /gene="yjaH"
                     /locus_tag="N7B39_001649"
     CDS             23792..24487
                     /gene="yjaH"
                     /locus_tag="N7B39_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HGU2421652.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHFLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24489..24914)
                     /gene="zraP"
                     /locus_tag="N7B39_001650"
     CDS             complement(24489..24914)
                     /gene="zraP"
                     /locus_tag="N7B39_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HGU2421653.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            25152..26528
                     /gene="zraS"
                     /locus_tag="N7B39_001651"
     CDS             25152..26528
                     /gene="zraS"
                     /locus_tag="N7B39_001651"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HGU2421654.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEVWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANCREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASLEGKGARFTLWLPVN
                     ITRKDPQG"
     gene            26525..27850
                     /gene="zraR"
                     /locus_tag="N7B39_001652"
     CDS             26525..27850
                     /gene="zraR"
                     /locus_tag="N7B39_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HGU2421655.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VRERVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHIIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMLVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LAQSLDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27847..29136)
                     /gene="purD"
                     /locus_tag="N7B39_001653"
     CDS             complement(27847..29136)
                     /gene="purD"
                     /locus_tag="N7B39_001653"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HGU2421656.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPTLQ
                     NVAIGVTDIPALLDFAQNEKVDLTIVGPEAPLVKGVVDTFRAAGMKIFGPTAGAAQLE
                     GSKAFTKDFLARHNIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDDVHQRTMERIIWPTVKGMASEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVEDGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(29148..30737)
                     /gene="purH"
                     /locus_tag="N7B39_001654"
     CDS             complement(29148..30737)
                     /gene="purH"
                     /locus_tag="N7B39_001654"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HGU2421657.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTKRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7311 a         8056 c         8524 g         7240 t
ORIGIN      
        1 ccgatcgaag acgtattctc catctccggt cgtggtaccg ttgttaccgg tcgtgtagaa
       61 cgcggtatca tcaaagttgg tgaagaagtt gaaatcgttg gtatcaaaga gactcagaag
      121 tctacctgta ctggcgttga aatgttccgc aaactgctgg acgaaggccg tgctggtgag
      181 aacgtaggtg ttctgctgcg tggtatcaaa cgtgaagaaa tcgaacgtgg tcaggtactg
      241 gctaagccgg gcaccatcaa gccgcacacc aagttcgaat ctgaagtgta cattctgtcc
      301 aaagatgaag gcggccgtca tactccgttc ttcaaaggct accgtccgca gttctacttc
      361 cgtactactg acgtgactgg taccatcgaa ctgccggaag gcgtagagat ggtaatgccg
      421 ggcgacaaca tcaaaatggt tgttaccctg atccacccga tcgcgatgga cgacggtctg
      481 cgtttcgcaa tccgtgaagg cggccgtacc gttggcgcgg gcgttgtagc aaaagttctg
      541 agctaattgc cgataacatt tgacgcaatg cgcactaaaa gggcatcatt tgatgccctt
      601 tttgcacgct ttcgtaccag aacctggctc atcagtgatt ttctttgtca taatcattgc
      661 tgagacaggc tctgttgagg gcgtataatc cgaaaagcta atacgcgttt cgatttggtt
      721 tgcctcgcga tcgcggggtg aaaatgtttg tagaaaactt ctgacaggtt ggtttatgag
      781 tgcgaatacc gaagctcaag gaagcgggcg cggcctggaa gcgatgaagt gggtcgttgt
      841 ggtggcattg ctcttggtgg cgattgtcgg caactatctt tatcgcgaca ttatgctgcc
      901 gctgcgtgcg ctggccgtag taattctgat tgctgcagcg ggtggtgtcg cgctgttaac
      961 gacaaaaggt aaagctaccg ttgcttttgc ccgtgaagcg cgtaccgaag tccgtaaggt
     1021 catttggccg actcgccagg aaacattgca caccacgctg attgtggctg cggttaccgc
     1081 agtaatgtca ctgatcctgt ggggactgga tggtattctg gttcgcctgg tatcctttat
     1141 cactggcctg aggttctgag atgtctgaag ctcctaaaaa gcgctggtac gtcgttcagg
     1201 cgttttccgg ttttgaaggc cgcgtagcaa cgtcgctgcg tgagcatatc aaattacaca
     1261 acatggaaga tttgtttggt gaagtcatgg taccaaccga agaagtggtt gaaatccgtg
     1321 gcggtcagcg tcgcaaaagc gaacgtaaat tcttccctgg ctacgtcctc gttcagatgg
     1381 tgatgaacga cgcgagctgg cacctggtgc gcagcgtacc gcgtgtgatg ggcttcatcg
     1441 gcggtacttc cgatcgtcct gcgccaatca gcgataaaga agtcgatgcg attatgaacc
     1501 gcctgcagca ggttggtgat aagccgcgtc cgaaaacgct gtttgaaccg ggtgaaatgg
     1561 tccgtgttaa tgatggtccg ttcgctgact tcaacggtgt tgttgaagaa gtggattacg
     1621 agaaatctcg tctgaaagtg tctgtttcta tcttcggtcg tgcgaccccg gtagagctgg
     1681 acttcagcca ggttgaaaaa gcctaaccca gcgatcaaaa aagcggcgat ttaatcgttg
     1741 cacaaggcgc gagattggaa tacaatttcg cgccttttgt ttttatgggt cttgcccgta
     1801 aaacgatttt ttatatcacg gggagcctct aagaggcgtt attacccaac ttgaggaatt
     1861 tataatggct aagaaagtac aagcctatgt caagctgcag gttgcagctg gtatggctaa
     1921 cccgagtccg ccagtaggtc cggctctggg tcagcagggc gtaaacatca tggaattctg
     1981 caaagcgttc aacgcaaaaa ctgattccat cgaaaaaggt ctgccgattc cggtagtaat
     2041 caccgtttac gctgaccgtt ctttcacttt cgttaccaag actccgccgg cagcagttct
     2101 gctgaaaaaa gcggctggta tcaagtctgg ttccggtaag ccgaacaaag acaaagtggg
     2161 taaaatttcc cgcgctcagc tgcaggaaat cgcgcagacc aaagctgccg acatgactgg
     2221 tgccgacatt gaagcgatga ctcgctccat cgaaggtact gcacgttcca tgggcctggt
     2281 agtggaggac taagaaatgg ctaaactgac caagcgcatg cgtgttatcc gcgagaaagt
     2341 tgatgcaacc aaacagtacg acatcaacga agctatcgct ctgctgaaag agctggcgac
     2401 tgctaaattc gtagaaagcg tggacgtagc tgttaacctc ggcatcgacg ctcgtaaatc
     2461 tgaccagaac gtacgtggtg caactgtact gccgcacggt actggccggt ccgttcgcgt
     2521 agccgtattt acccagggtg caaacgctga agctgctaaa gctgcaggtg ctgaactggt
     2581 aggtatggaa gatctggctg accagatcaa gaaaggcgaa atgaactttg acgttgttat
     2641 tgcttctccg gatgcaatgc gcgttgttgg ccagctgggc caggttctgg gtccgcgcgg
     2701 cctgatgcca aacccgaaag tgggtactgt aacgccgaac gttgctgaag cggttaaaaa
     2761 cgctaaagct ggccaggttc gttaccgtaa cgacaaaaac ggcatcatcc acaccaccat
     2821 cggtaaagtg gactttgacg ctgacaaact gaaagaaaac ctggaagctc tgctggttgc
     2881 gctgaaaaaa gcaaaaccga ctcaggcgaa aggcgtgtac atcaagaaag ttagcatctc
     2941 caccaccatg ggtgcaggtg ttgcagttga ccaggctggc ctgagcgctt ctgtaaacta
     3001 atgcctttac gtgggcggtg attttgtcta caatcttacc cccacgtatg ctaacgaaag
     3061 ttagcagcta aaaagatttg ttcgttggag cctggcctat ccaggcctcc gtcgaagacc
     3121 gcaggagttt cgcaagaaac ttaatcccct gcgtagacgg tgacagaacg ctaagattat
     3181 tcttttatat tctggcttgt ttctgctcac cgtaattaag acgctctctc cgtttggagg
     3241 agtgaagtga gttccagaga ttttctctgg caaacatcca ggagcaaagc taatggcttt
     3301 aaatcttcaa gacaaacaag cgattgttgc tgaagtcagc gaagtagcca aaggcgcgct
     3361 gtctgcagta gttgcggatt cccgtggcgt aactgtagat aaaatgactg aactgcgtaa
     3421 agcaggtcgc gaagctggcg tatacatgcg tgttgttcgt aacaccctgc tgcgccgtgc
     3481 tgttgaaggt actccgttcg agtgcctgaa agacgcgttt gttggtccga ccctgattgc
     3541 atactctatg gaacacccgg gcgctgctgc tcgtctgttc aaagagttcg cgaaagcgaa
     3601 tgcaaaattt gaggtcaaag ccgctgcctt tgaaggtgag ctgatcccgg cgtctcagat
     3661 cgaccgcctg gcaactctgc cgacctacga agaagcaatt gcacgcctga tggcaaccat
     3721 gaaagaagct tcggctggca aactggttcg tactctggct gctgtacgcg atgcgaaaga
     3781 agctgcttaa tcgcagttat ctttttaacg cattcgctta cgtataaact tattctgata
     3841 ttcaggaaca atttaaatgt ctatcactaa agatcaaatc attgaagcag ttgcagctat
     3901 gtctgtaatg gacgttgtag aactgatctc tgcaatggaa gaaaaattcg gtgtttccgc
     3961 tgctgctgct gtagctgtag ctgctggccc ggttgaagct gctgaagaaa aaactgaatt
     4021 cgacgtaatt ctgaaagctg ctggcgctaa caaagttgct gttatcaaag cagtacgtgg
     4081 cgcaactggc ctgggtctga aagaagctaa agacctggta gaatctgcac cggctgctct
     4141 gaaagaaggc gtgagcaaag acgacgcaga agcactgaaa aaagctctgg aagaagctgg
     4201 cgctgaagtt gaagttaaat aagccaaccc ttccggttgc agcctgagaa atcaggctga
     4261 tggctggtga ctttttagtc accagccttt ttgcgctgta aggcaccagt agcgtttcac
     4321 actgtttgac tactgctgtg cctttcaatg cttgtttcta tcgacgactt aatatactgc
     4381 gacaggacgt ccgttctgtg taaatcgcaa tgaaatggtt taagcgtgat agcaacaggc
     4441 attgcggaaa gtgttccatt ttccggtcaa caaaatagtg ttgcacaaac tgtccgctca
     4501 atggacagat gggtcgactt gtcagcgagc tgaggaaccc tatggtttac tcctataccg
     4561 agaaaaaacg tattcgtaag gattttggta aacgtccaca agttctggat gtaccttatc
     4621 tcctttctat ccagcttgac tcgtttcaga aatttatcga gcaagatcct gaagggcagt
     4681 atggtctgga agctgctttc cgttccgtat tcccgattca gagctacagc ggtaattccg
     4741 agctgcaata cgtcagctac cgccttggcg aaccggtgtt tgacgtccag gaatgtcaaa
     4801 tccgtggcgt gacctattcc gcaccgctgc gcgttaaact gcgtctggtg atctatgagc
     4861 gcgaagcgcc ggaaggcacc gtaaaagaca ttaaagaaca agaagtctac atgggcgaaa
     4921 ttccgctcat gacagacaac ggtacctttg ttatcaacgg tactgagcgt gttatcgttt
     4981 cccagctgca ccgtagtccg ggcgtcttct ttgactccga caaaggtaaa acccactctt
     5041 cgggtaaagt gctgtataac gcgcgcatca tcccttaccg tggttcctgg ctggacttcg
     5101 aattcgatcc gaaggacaac ctgttcgtac gtatcgaccg tcgccgtaaa ctgcctgcga
     5161 ccatcattct gcgtgccctg aactacacca cagagcagat cctcgacctg ttctttgaaa
     5221 aagttatctt tgaaatccgt gataacaagc tgcagatgga actggtgccg gaacgcctgc
     5281 gtggtgaaac cgcatccttt gacatcgaag ctaacggtaa agtgtacgta gaaaaaggcc
     5341 gccgtatcac tgcgcgccac attcgccagc tggaaaaaga cgacgtcaaa ctgatcgaag
     5401 tcccggttga gtacatcgca ggtaaagtgg ttgctaaaga ctatattgat gagtctaccg
     5461 gcgagctgat ctgcgcagcg aacatggagc tgagcctgga tctgctggct aagctgagcc
     5521 agtctggtca caagcgtatc gaaacgctgt tcaccaatga tctggatcac ggcccgtata
     5581 tctctgaaac cttacgtgtc gacccaacta acgaccgtct gagcgcactg gtagaaatct
     5641 accgcatgat gcgccctggc gagccgccga ctcgtgaagc agcggaaagc ctgttcgaga
     5701 acctgttctt ctccgaagac cgttatgacc tgtctgcggt tggtcgtatg aagttcaacc
     5761 gttctctgct gcgcgaagaa atcgaaggtt ctggtatcct gagcaaagac gacatcattg
     5821 atgttatgaa aaagctcatc gatatccgta acggtaaagg cgaagtcgat gatatcgacc
     5881 acctcggcaa ccgtcgtatc cgttccgttg gcgaaatggc ggaaaaccag ttccgcgttg
     5941 gcctggtacg tgtagagcgt gcggtgaaag agcgtctgtc tctgggcgat ctggataccc
     6001 tgatgcctca ggatatgatc aacgccaagc cgatttccgc agcagtgaaa gagttcttcg
     6061 gttccagcca gctgtctcag tttatggacc agaacaaccc gctgtctgag attacgcaca
     6121 aacgtcgtat ctccgcactc ggcccaggcg gtctgacccg tgaacgtgca ggcttcgaag
     6181 ttcgagacgt acacccgact cactacggtc gcgtatgtcc aatcgaaacc cctgaaggtc
     6241 cgaacatcgg tctgatcaac tctctgtccg tgtacgcaca gactaacgaa tacggcttcc
     6301 ttgagactcc gtatcgtaaa gtgactgacg gtgttgtaac tgacgaaatt cactacctgt
     6361 ctgctatcga agaaggcaac tacgttatcg cccaggcgaa ctccaacctg gatgaagaag
     6421 gccacttcgt agaagacctg gtaacctgcc gtagcaaagg cgaatccagc ttgttcagcc
     6481 gtgaccaggt tgactacatg gacgtatcca cccagcaggt ggtatccgtc ggtgcgtccc
     6541 tgatcccgtt cctggaacac gatgacgcca accgtgcatt gatgggtgcg aacatgcaac
     6601 gtcaggccgt tccgactctg cgtgctgata agccgctggt tggtactggt atggaacgtg
     6661 ctgttgccgt tgactccggt gtaactgcgg ttgctaaacg tggtggtgtc gttcagtacg
     6721 tggatgcttc ccgtatcgtt atcaaagtta acgaagacga gatgtatccg ggtgaagcag
     6781 gtatcgacat ctacaacctg accaaataca cccgttctaa ccagaacacc tgtattaacc
     6841 agatgccgtg tgtgtctctg ggtgaaccgg ttgaacgtgg cgacgtgctg gcagacggtc
     6901 cgtccaccga ccttggtgaa ctggcgcttg gtcagaacat gcgcgtagcg ttcatgccgt
     6961 ggaatggtta caacttcgaa gactccatcc tcgtatccga gcgtgttgtt caggaagacc
     7021 gtttcaccac catccacatt caggaactgg cgtgtgtgtc ccgtgacacc aagctggggc
     7081 cagaagagat caccgctgac atcccgaacg tgggtgaagc tgcgctctcc aaactggatg
     7141 aatccggtat cgtttatatt ggtgcggaag tgaccggtgg cgacattctg gttggtaagg
     7201 ttacgccgaa aggtgaaact cagctgaccc cagaagaaaa actgctgcgt gcgatcttcg
     7261 gtgagaaagc gtctgacgtt aaagactctt ctctgcgcgt accaaacggt gtatccggta
     7321 cggttatcga cgttcaggtc tttactcgcg atggcgtaga aaaagacaaa cgtgcgctgg
     7381 aaatcgaaga aatgcagctc aaacaggcga agaaagacct gtctgaagaa ctgcagatcc
     7441 tcgaagcggg tctgttcagc cgtatccgtg ctgtgctggt agccggtggc gttgaagctg
     7501 agaagctcga caaattgccg cgcgatcgct ggctggagct gggcctgacc gacgaagaga
     7561 aacaaaatca gctggaacag ctggctgagc agtatgacga actgaaacac gagttcgaga
     7621 agaaactcga agcgaaacgc cgcaaaatca cccagggcga cgatctggca ccgggcgtgc
     7681 tgaagattgt taaggtatat ctggcggtta aacgccgtat ccagcctggt gacaagatgg
     7741 caggtcgtca cggtaacaag ggtgtaattt ctaagatcaa cccgatcgaa gatatgcctt
     7801 acgatgaaaa cggtacgccg gtagacatcg tactgaaccc gctgggcgta ccgtctcgta
     7861 tgaacatcgg tcagatcctc gaaacccact tgggtatggc tgcgaaaggt atcggcgaca
     7921 agatcaacgc catgctgaaa cagcagcagg aagtcgcgaa actgcgtgaa ttcatccagc
     7981 gtgcgtacga tctgggcgct gacgttcgtc agaaagttga cctgagtacc ttcagcgatg
     8041 aagaagttat gcgtctggct gaaaacctgc gcaaaggtat gccaatcgca acgccggtgt
     8101 tcgacggtgc gaaagaagca gaaattaaag agctgctgaa acttggcgac ctgccgactt
     8161 ctggtcagat ccgcctgtac gacggccgca ctggtgaaca gttcgaacgt ccggtaaccg
     8221 ttggttacat gtacatgctg aaactgaacc acctggtcga cgacaagatg cacgcgcgtt
     8281 ccaccggttc ttacagcctg gttactcagc agccgctggg tggtaaggca cagttcggtg
     8341 gtcagcgttt cggggagatg gaagtgtggg cgctggaagc atacggcgca gcatacaccc
     8401 tgcaggaaat gctcaccgtt aagtctgatg acgtgaacgg tcgtactaag atgtataaaa
     8461 acatcgtgga cggcaaccat cagatggagc cgggcatgcc agaatccttc aacgtattgt
     8521 tgaaagagat tcgttcgctg ggtatcaaca tcgaactgga agacgagtaa ttctcgctca
     8581 aacaggtcac tgctgtcggg gtaaaacccg gcagcggatt gtgctaactc cgacgggagc
     8641 aaatccgtga aagatttatt aaagtttctg aaagcgcaga ctaaaaccga agagtttgat
     8701 gcgatcaaaa ttgctctggc ttcgccagac atgatccgtt catggtcttt cggtgaagtt
     8761 aaaaagccgg aaaccatcaa ctaccgtacg ttcaaaccag aacgtgacgg ccttttctgc
     8821 gcccgtatct ttgggccggt aaaagattac gagtgcctgt gcggtaagta caagcgcctg
     8881 aaacaccgtg gcgtcatctg tgagaagtgt ggcgttgaag tgacccagac taaagtacgc
     8941 cgtgagcgta tgggccacat cgaactggct tccccgactg cgcacatctg gttcctgaaa
     9001 tcgctgccgt cccgtatcgg cctgctgctc gatatgccgc tgcgcgatat cgaacgcgta
     9061 ctgtactttg aatcctatgt ggttatcgaa ggcggtatga ccaacctgga gcgtcagcag
     9121 atcctgactg aagagcagta tctggacgcg ctggaagagt tcggtgacga attcgacgcg
     9181 aaaatgggtg cggaagcaat ccaggccctg ctgaagagca tggatctgga gcaagagtgc
     9241 gaacagctgc gtgaagagct gaacgaaacc aactccgaaa ccaagcgtaa aaagctgacc
     9301 aagcgtatca aactgctgga agcgttcgtt cagtctggta acaaaccaga gtggatgatc
     9361 ctgaccgttc tgccggtact gccgccagat ctgcgtccgc tggttccgct ggatggtggt
     9421 cgtttcgcga cttctgacct gaacgatctg tatcgtcgcg tcattaaccg taacaaccgt
     9481 ctgaaacgtc tgctggatct ggctgcgccg gacatcatcg tacgtaacga aaaacgtatg
     9541 ctgcaggaag cggtagacgc cctgctggat aacggtcgtc gcggtcgtgc gatcaccggt
     9601 tctaacaagc gtcctctgaa atctttggcc gacatgatca aaggtaaaca gggtcgtttc
     9661 cgtcagaacc tgctcggtaa gcgtgttgac tactccggtc gttctgtaat caccgtgggt
     9721 ccatacctgc gtctgcatca gtgcggtctg ccgaagaaaa tggcgctgga gctgttcaaa
     9781 ccgttcatct acggcaagct ggaactgcgt ggtcttgcta ccaccattaa agctgcgaag
     9841 aaaatggttg agcgcgaaga agctgtcgtt tgggatatcc tggacgaagt tatccgcgaa
     9901 cacccggtac tgctgaaccg tgcaccgact ctgcaccgtc tgggtatcca ggcatttgaa
     9961 ccggtactga tcgaaggtaa agctatccag ctgcacccgc tggtttgtgc ggcatataac
    10021 gccgacttcg atggtgacca gatggctgtt cacgtaccgc tgacgctgga agcccagctg
    10081 gaagcgcgtg cgctgatgat gtctaccaac aacatcctgt ccccggcgaa cggcgaacca
    10141 atcatcgttc cgtctcagga cgttgtactg ggtctgtact acatgacccg tgactgtgtt
    10201 aacgccaaag gcgaaggcat ggtgctgact ggcccgaaag aagcagaacg tctgtatcgc
    10261 tctggtctgg cttctctgca tgcgcgcgtt aaagtgcgta tcaccgagta tgaaaaagat
    10321 gctaacggtg aattagtagc gaaaaccagc ctgaaagaca cgactgttgg ccgtgccatt
    10381 ctgtggatga ttgtaccgaa aggtctgcct tactccatcg tcaaccaggc gctgggtaaa
    10441 aaagcaatct ccaaaatgct gaacacctgc taccgcattc tcggtctgaa accgaccgtt
    10501 atttttgcgg accagatcat gtacaccggc tttgcatatg cagcgcgttc tggtgcatct
    10561 gttggtatcg atgacatggt catcccggag aagaaacacg aaatcatctc cgaggcagaa
    10621 gcagaagttg ctgaaattca ggagcagttc cagtctggtc tggtaactgc gggcgaacgc
    10681 tacaacaaag ttatcgatat ctgggctgcg gcgaacgatc gtgtatccaa agcgatgatg
    10741 gataacctgc aaactgaaac cgttattaac cgtgacggtc aggaagagaa gcaggtttcc
    10801 ttcaacagca tctacatgat ggccgactcc ggtgcgcgtg gttctgcggc acagattcgt
    10861 cagcttgctg gtatgcgtgg tctgatggcg aagccggatg gctccatcat cgaaacgcca
    10921 atcaccgcga acttccgtga aggtctgaac gtactccagt acttcatctc cacccacggt
    10981 gctcgtaaag gtctggcgga taccgcactg aaaactgcga actccggtta cctgactcgt
    11041 cgtctggttg acgtggcgca ggacctggtg gttaccgaag acgattgtgg tacccatgaa
    11101 ggtatcatga tgactccggt tatcgagggt ggtgacgtta aagagccgct gcgcgatcgc
    11161 gtattgggtc gtgtaactgc tgaagacgtt ctgaagccgg gtactgctga tatcctcgtt
    11221 ccgcgcaaca cgctgctgca cgaacagtgg tgtgacctgc tggaagagaa ctctgtcgac
    11281 gcggttaaag tacgttctgt tgtatcttgt gacaccgact ttggtgtatg tgcgcactgc
    11341 tacggtcgtg acctggcgcg tggccacatc atcaacaagg gtgaagcaat cggtgttatc
    11401 gcggcacagt ccatcggtga accgggtaca cagctgacca tgcgtacgtt ccacatcggt
    11461 ggtgcggcat ctcgtgcggc tgctgaatcc agcatccagg tgaaaaacaa aggtagcatc
    11521 aagctcagca acgtgaagtc ggttgtgaac tccagcggta aactggttat cacttcccgt
    11581 aacaccgaac tgaaactgat cgacgaattc ggtcgtacca aagaaagcta caaagtacct
    11641 tacggtgcgg tactggcaaa aggcgatggc gaacaggttg ctggcggcga aaccgttgca
    11701 aactgggacc cgcacaccat gccggttatc accgaagtaa gcggttttgt acgctttact
    11761 gacatgatcg acggccagac cattactcgt cagaccgacg aactgaccgg tctgtcttcg
    11821 ctggtggttc tggattccgc agaacgtacc gcaggtggta aagatctgcg tccggcactg
    11881 aaaatcgttg atgctcaggg taacgacgtt ctgatcccag gtaccgatat gcctgcgcag
    11941 tacttcctgc cgggtaaagc gattgttcag ctggaagatg gcgtacagat cagctctggt
    12001 gacaccctgg cgcgtattcc gcaggaatcc ggcggtacca aggacatcac cggtggtctg
    12061 ccacgcgttg cggacctgtt cgaagcacgt cgtccgaaag agccggcaat cctggctgaa
    12121 atcagcggta tcgtttcctt cggtaaagaa accaaaggta aacgtcgtct ggttatcacc
    12181 ccggtagacg gtagcgatcc gtacgaagag atgattccga aatggcgtca gctcaacgtg
    12241 ttcgaaggtg aacgtgtaga acgtggtgac gtaatttccg acggtccgga agcgccgcac
    12301 gacattctgc gtctgcgtgg tgttcatgct gtgactcgtt acatcgttaa cgaagtacag
    12361 gacgtatacc gtctgcaggg cgttaagatt aacgataaac acatcgaagt tatcgttcgt
    12421 cagatgctgc gtaaagctac catcgttaac gcgggcagct ccgacttcct ggaaggcgaa
    12481 caggttgaat actctcgcgt caagatcgca aaccgcgaac tggaagcgaa cggcaaagtg
    12541 ggtgcaactt actcccgcga tctgctgggt atcaccaaag cgtctctggc aaccgagtcc
    12601 ttcatctccg cggcatcgtt ccaggagacc actcgcgtgc tgaccgaagc agccgttgcg
    12661 ggcaaacgcg acgaactgcg cggcctgaaa gagaacgtta tcgtgggtcg tctgatcccg
    12721 gcaggtaccg gttacgcgta ccaccaggat cgtatgcgtc gccgtgctgc gggtgaagct
    12781 ccggctgcac cgcaggtgac tgcagaagac gcatctgcca gcctggcaga actgctgaac
    12841 gcaggtctgg gcggttctga taacgagtaa tcgttaatcc gcaaataacg taaaaacccg
    12901 cttcggcggg tttttttatg gggggagttt agggaaagag catttgtcag aatatttaag
    12961 gaatttctga atactcataa tcaatgtaga gatgactaat atcctgaaac tgactgaact
    13021 aattgagtca aactcggcaa ggattcgata ctattcctgt gtagctttct taaggaacga
    13081 gaatgaaaca ggaagtggag aagtggcgac cttttaaaca tccggatggc gacgttcgtg
    13141 atttatcgtt tcttgatgct catcaggtta tctacgttca gcatcatgag gacaaggagc
    13201 ctttagagta tcgcttttgg gttacctact ctcttcactg cttcacaaaa gattatgaac
    13261 atcagacgaa cgaagaaaaa ctatcgttaa tgtaccacgc gcctaaagaa tctcgtccct
    13321 tctgccagca ccgttataac ttagcgcgca cacacttaaa aagaactatt ttggcgctgc
    13381 cagaaagcaa cgttattcat gccgggtatg gtagctatgc cgtgattgag gtggacttag
    13441 acggaggaga taaggcattt tactttgttg tgttcagggc tttcagtgaa atttttcacc
    13501 attgcctaaa acttattgag aaataagcag cttcctcagc cctcaaaata acaaaaccca
    13561 ccttaaggtg ggtttcgcca gagaattatc tctggtattc agaacgccat taccggactt
    13621 tgccttgacc ttgcgataat cgcaggttgc gggatgtctg aatttcttca gtctgctgca
    13681 tcctggaaga tgagaacatg tgttcttatt ttcgtctcta tcatagttga gtatttactc
    13741 tcttacaatc agatctcttt cattgctcaa caggcgatgg cttcagactt tgcattacgg
    13801 aatttttaag aaaggcaggg cgaaacgagg aagaagcttt tcgccctgga caacattcct
    13861 gctgacggca ctaccataaa aaaacacaac gctttcaaca accttgcgtt tttcatcttc
    13921 cgtagcggct cggaataaat tttcgttctt acattccgtc tcatagtctt tgcgaggcgc
    13981 gtcccagata gctgtcccag tctttccata ccggctgcaa accctgagcg gttaacgcgg
    14041 cagcaaccgc ttccggtctg cgctcgtcgt gcggtgagaa ctgttccagc tcggggtgat
    14101 tgtcggcata gccacctggc tgcgttttcg aaaaagcgct gacgttatta attgccagcg
    14161 gaataacgcg atcgcgaaac cacggtgatt cccgcgtgga gagtgacagt tcaatctccg
    14221 gtgcaagcag ccggaaggcg cagatggttt gcactaactg gcgttcatcc ataatcgacg
    14281 caggctcaat gccgccagta catggacgca ggcgtgggaa ggagacagag tagcggcttt
    14341 gccagtaatg ctgttgcagc catagcaaat gttctgcaac catatagcag tcaacgcgcc
    14401 agctgtcgga aaggccaatt agcgcgccga ggcctatctt atcaatcccc gcacggccca
    14461 gccgatccgg cgtttccagc cgccagaaga agtcctgttt tttgcctttc agatgatggc
    14521 gggcataagt cgcctcgtga tatgtctcct gataaaccat cacgccatcc agaccaagtt
    14581 gctttaactc ggcgtattcc gtctccgcca gcggctgtac ttccatctgt agtgaagaga
    14641 actgttcacg cagggcaggg agatgacgac gaaagtaatc catccccact ttcgcctgat
    14701 gttcaccggt gactaaaagc agatgttcaa agcccatctc ccgtatagcg gcgctttccc
    14761 tggcaatatc cgcttcatcc agcgttttgc gcttgatgcg gttgctcata gagaatccgc
    14821 agtacgtgca gtcgttagcg caaagattgg aaagataaag cgggacgtag aaactaactg
    14881 tgttgccaaa tcgctgacgg gtcagacgct gcgcccgttg ggccagttgt tccagatagc
    14941 cactggcggc tggcgataac agcgccatca tatcgtcgcg ggtgagttgc gaggcattta
    15001 gcgcccgctc tacgtcagca gccgttttgc cgttgatacg caggcggatg tcgtcccagt
    15061 ccagttgtcg ccagcgatcg ctgaaggttt tcataccgat gcctccagaa atccggtcag
    15121 cgggctggtg gcatgagcaa aataactgcg gctgcccggt ccggactgag gtgccagcag
    15181 gcctgcttct actgccagac gaaatgcctt cgccatgttg acgggatcgt ccgcgacggc
    15241 aatcgccgta ttcactaaca ccgcgtcggc ccccatttcc agcgcctgcg cggcatggct
    15301 gggaacgccg atgccagcat cgacaaccac cggaactgtg gcctgctgga taataatctc
    15361 cagcatggcg cgggtttcca gcccctgatt cgagccaatc ggcgcgccga gcggcatcac
    15421 cgctgcacag ccgacttctt ccagacgttt acacaatacc ggatcggccc cgcagtaagg
    15481 cagcacgaca aatccctgtt gtaccagcgt ttcggcggct ttcagggttt cgatgggatc
    15541 gggcaacagc cagcgggcgt cagggtgaat ctccaatttt aaccagtttg tgcctaacgc
    15601 ttcacgagcc agatgggcgg cgaaaatggc ttcttccgct gttttcgccc cggatgtatt
    15661 tggcagcagg gtcacacccg ccgcgataag cggttcgagg atagcgtcgt tgtgctggcg
    15721 caagtcgaca cgtttcatcg ccagcgtcac cagctggctg ccggaagcgc ggattgcctc
    15781 taccatcagt tgtgaagagg cgaatttccc ggtgccggta aacagatgtg aatcaaacgt
    15841 tttgtccgca atacgtaaca tctcaacccc ctgcaataac ctgaaaaagt aggatctggt
    15901 cgccatcctg cacgatatgt tgcgtccact gctcacgcgg gacgatttgc tgattaatcg
    15961 ccagagccgc gcccgcttgt cgttgaacga gttgctccag tagttcgtga acagtttgcc
    16021 cggcggcgca ctgcatcggt tgatcgttaa acaggatctg cattgcttcc tccgcatacc
    16081 gggcaaccac tggcgcggcg caacgccagg ctgcgccact ggctcgactt accgtcgaac
    16141 agtcggagtt ctcccgcagg cgtctctata ccgcttaata acttaatggc ttccagtgcc
    16201 tgcaaagtgc ccataacccc gaccaccggg ccaaccacgc ccgccgtgcg gcagttgcgt
    16261 tctggctcct ggttttctgg ccacaggcag cggtaacaac cctgccccca gggcggcgtc
    16321 agtaccatca actgaccgcc aaatccgacc gcgctggcgg tgataagcgg cgtgttgagt
    16381 gccacgcagg cggcattaat ctcctggcga gtcgccatat tgtcggtaca gtcgagcacc
    16441 acatcggctt gtgcaaccgc atcttttaac gcctcacccg ttaaccgttg ttgtaatgct
    16501 gtcagttgaa tatcgggatt caactgtgtc agtcgctgat ggctgacctg cgatttcggg
    16561 cgatcgatat cttcagtggt aaagaggatt tgtcgttgca gattgcttaa atgcacatcg
    16621 tcgtcatctg ccagtaccag cgtcccgacg ccagcgcccg ccaggtatag cgcagcaggt
    16681 gtacccagcc cgccaagacc gataatcagc acctggctgt cgagcagttt ttgctgcccg
    16741 tcaagagcga tatcgtcgag caggatttgg cggctataac gcataaaatc acggtcattc
    16801 atcgccaact cctgcaattt ccagcaactg tgccgttgcc aaacgccagt ctgcggcttg
    16861 agtaatggcg ctgacgacgg cgatactgcc gacacccgtt gctatcaccg caggcgcgcg
    16921 tgccagactg ataccgccaa tcgccacggt gggataatcc gacagtcgct caacatgccg
    16981 tgccagctgt tccagcccct gcggtgcaga aggcatctgt ttggtttgcg tcgggaacac
    17041 atgtcccagc gcgatataag aggggcgtgc tgccagcgcg acgtcgattt ccatatcgtc
    17101 atgggtcgaa acgcccagcc gcaggcctgc cgcgcggatg gcattgagat cggtggcttg
    17161 caaatcttcc tgccccaaat ggacgccata cgcctgatgc ttgatcgcga gccgccagta
    17221 atcgttgata aacaatcgcg cgttatagcg gcggcccagc gcaattgccg ccacgacatc
    17281 ggcttccacc tcttcatcgc gccgatcttt gatgcgtagc tggagagtac gtacgcctgc
    17341 atccaacaga cgttcgatcc actgtacgct gtccaccacc gggtacagtc ctaaacgaaa
    17401 aggtacagga ggaaaatcag gctgatacat cacgcttcct ccttacgcag gtagatttct
    17461 ccgcctctgg cacggaagtt ctccgacata tccgctattc ccatttcaat agtttgcgtg
    17521 gcggcgtaat cacgcacttc ctggctgatt ttcatcgagc agaatttcgg cccacacatg
    17581 gagcaaaaat gggcgacttt acctgactct tgcggcaggg tttcatcgtg ataagcgcgg
    17641 gcggtaaacg ggtcgagggc cagattaaac tggtcttccc agcgaaattc gaagcgggct
    17701 ttcgacatgg cgttatcgcg aatttgtgcg cccggatgcc ctttcgccag gtcagcggcg
    17761 tgggcggcaa tcttataggt gataagcccc tgcttaacat cttctttatt aggcagaccc
    17821 agatgctctt ttggcgttac gtaacagagc atcgcgcagc caaaccagcc aatcatcgcc
    17881 gcaccaatcc ccgacgtgaa gtggtcatag cccggcgcaa tatcggtagt tagcggcccc
    17941 agagtgtaaa acggcgcttc gtggcagtgc tctaactcct cggtcatatt gcggcggatc
    18001 atctgcatcg gcacgtggcc tgggccttca atcatcacct gcacatcata ttcccaggcg
    18061 atttttgtca gttcgcccag cgtatgcagc tcggcaaact gcgcttcatc gttggcgtcc
    18121 tgaatagaac cggggcgcag accgtcgccc agcgacagcg aaacgtcata agcggcacag
    18181 atttcacaaa tttcgcggaa gtgttgatag aggaaatttt cctgatgatg ggagaggcac
    18241 catttcgcca taatcgaacc gccgcgcgag acgataccgg tcaggcgttt cgcggtcatc
    18301 ggcacatagc gcagcagcac ccccgcatgg atagtgaagt aatccacacc ttgctcggcc
    18361 tgttccagca gcgtgtcgcg gaacgcttcc caggtaagat cttcggcgat cccattaacc
    18421 ttctccagcg cctggtagat cggcactgta ccaatcggca ccgggctgtt acgcaaaatc
    18481 cactcgcggg tttcgtgaat atagcgaccg gtggagagat ccatcaccgt atccgctccc
    18541 cagcgcgtgg accataccag cttttccact tcttcttcga tggaagaggt gaccgccgag
    18601 ttgccgatat tggcgttaac ttttaccagg aaattgcgac caataatcat cggctccgat
    18661 tccggatgat taatgttggc cgggataatc gcacgtccgg cagcaacttc atcacggaca
    18721 aattccgcag tgatattttc cggcagacgt gcgccaaagc tcattcccgg atgctggtgg
    18781 cgtaaaactt cgctacggat gcgctcgcgg cccatattct cgcggatggc gatgaattcc
    18841 atttccggtg tgatgatgcc ctggcgggcg tagtgcagtt gtgtgacgcg gcgtcctgct
    18901 ttggcgcgtt ttggcgttaa tacgccgcta aaacgcagct cgtcgaggcc atcatctgcc
    18961 agccgcgctt tagtgtaatc ggaactgcgc acggtaagtt cttcggtatc gccgcgcgca
    19021 tcgatccacg gctggcgtag ttttgccagc ccttgctgca cgttaatggc gatctgcgga
    19081 tcgccatacg ggccggaggt gtcgtagacc ggaatcgctt cgttttcttc gtactgcggc
    19141 tgttctttgc taccgccaat tagcgtcggg ctaagctgga tctcacgcat cggcacgcgc
    19201 acgccgggtt gtgtgccagt gatataaatg cgttttgagt tgggaaaggc ggtgccttcc
    19261 agggtgtcga taaaatgttg ggcctgggcg cgttgttcgc gacgggtcag ttttgttgca
    19321 gacatagctc attccaaaaa gttaaggacg tggcttgtca gacgacggat gaagcaagag
    19381 acgatcgccg caaaggcgat gcgatagcag attaactctt gttcccttcg caggtattag
    19441 cctgatcagg ttccgcggat cccgaataaa cggtctcagc cagttaaggc actccgacaa
    19501 gaaattagcc ccacaaacgg ggcgttgaat gtaaattacg cgttaacagc gcagaactca
    19561 agcaggatgt ttgacgcggg cggcgtcaag caccagcaga atcagcttat cttccagcac
    19621 aaagcgcgct tccagcgcct cgccgatgtc agataaaacc tgttgaaact caaggtaatt
    19681 atcatgatcg atagcggttt ccaggctgga atcgtagtaa tccataatct gttgggtatt
    19741 ggcttcgagt tgcggccaaa tcttcgcggc gcgcgcgagt tgcccgttgc cttccagctt
    19801 atgaagaatg cgctcataaa tactgaaatg tccggcagac aagtaatcga ccaggctctg
    19861 acaaaaatca tcaagggctt tttcgtttag cctcatgtac gattctttgc caggcttaat
    19921 gccaaccaga ttgtagtaag ccacgagcag atgcttacgt acatgtagcc agcgatcaac
    19981 cagtttgtta cttcctctga cgcgctccgt caggttatcg agctggttaa gcatgattga
    20041 ctccgcaagt ttgtattcaa aaactgctca gtgagaaatg taaaaaccat gttaaacatg
    20101 ccagtgatgc aaaggtagtg caagagctat ggatcgtata attgaaaaat tagatcacgg
    20161 ctggtgggtc gtcagccatg aacaaaaatt atggttgccg aagggagaat tgccatatgg
    20221 cgaagcggca aatttcgatc ttgtgggtca gcgcgcacta caaatcggcg aatggcaggg
    20281 ggaacctgtt tggttaatac aacagcagcg gcgttacgat atggggtcgg tacgtcaggt
    20341 cattgatctc gatgttgggc tgtttcaact ggccggacgt ggcgtacaac tggcggagtt
    20401 ttaccgatcg cataaatact gtggttactg cgggcatgaa atgtatccaa gcaaaaccga
    20461 atgggcgatg ctgtgcagcc attgccgtga gcgttactac ccgcaaatcg ccccctgcat
    20521 tattgttgcc atccgtcgcg atgattcgat cctcctcgcc cagcataccc gccatcgtaa
    20581 cggtgtccat acggtacttg ccggattcgt cgaagtgggc gaaaccctcg agcaggcggt
    20641 cgcgcgggaa gtgatggaag agagcggaat taaagttaaa cacttgcgtt atgtgacttc
    20701 ccagccgtgg ccgtttcctc agtctttaat gaccgcgttt atggcggaat atgacagcgg
    20761 cgacatcgtg atcgacccga aagaattgct cgaggcgaac tggtatcgct atgacgattt
    20821 gccgttactc ccgccgcccg gcaccgtagc gcgccgtctg atagaagata cggtggcgat
    20881 gtgtcgggca gagtatgagt gatgatacac tgaccgcctg acgcactaag gaacagccaa
    20941 aatgaccgaa cttaaaaacg atcgttatct gcgggcgctg ctgcgccagc ccgttgatgt
    21001 cactcctgta tggatgatgc gccaggcggg tcgctatcta ccggaatata aagccacgcg
    21061 cgcccaggcg ggcgatttta tgtcgctgtg caaaaacgcc gagctggcgt gtgaagtgac
    21121 cttgcaaccg ctgcgtcgct acccgctgga tgcggcgatc ctcttttccg atatcctcac
    21181 cgtcccggac gcgatggggt tagggctcta ttttgaagcc ggagaaggtc cgcgttttac
    21241 ctcgccagtc acctgcaaag ctgacgtcga taaactgcca attccggacc cggaagatga
    21301 actggggtac gtgatgaacg cggtgcgtac cattcgtcgc gaactgaaag gcgaagtgcc
    21361 gctgattggt ttttccggca gcccgtggac gctggcgacc tacatggtgg aaggcggcag
    21421 cagcaaagcc ttcaccgtga tcaaaaaaat gatgtatgcc gatccgcagg cgctgcacgc
    21481 tctactcgat aaactggcga aaagcgtcgc tttgtatctg aatgcgcaga ttaaagccgg
    21541 tgctcaggct gtgatgattt tcgacacctg gggcggcgtg cttaccgggc gcgattatca
    21601 acagttctcg ctttattaca tgcataaaat tgttgatggt ttactgcgtg aaaacgacgg
    21661 tcgccgcgta ccggtcacgc tgtttaccaa aggcggcgga cagtggctgg aagccatggc
    21721 agaaaccggt tgcgatgcgc tgggcctcga ctggacaacg gacatcgccg atgcgcgccg
    21781 ccgtgtgggc aataaagtcg cgttgcaggg taatatggat ccgtcgatgc tgtacgcgcc
    21841 gcctgcccgc attgaagaag aagtagcgac tatacttgca ggtttcggtc acggcgaagg
    21901 tcatgtcttt aaccttggtc acggcattca tcaggatgtg ccgccagaac atgctggcgt
    21961 gttcgtggag gcagtgcatc gactgtctga acagtatcac cgctaaggag cgattatgga
    22021 tctcgcgtca ttacgcgctc aacaaatcga actggcttct tctgtgatcc gcgaggatcg
    22081 actcgataag gatccgccgg atctgatcgc cggagccgat gtcgggtttg agcagggcgg
    22141 agaagtgacg cgagcggcga tggtgctgct gaaatacccc tcgcttgagc tggtggagta
    22201 taaagtggcc cgcatcgcta ccaccatgcc ttacattcca ggttttcttt ccttccgcga
    22261 atatcctgcg ctgctggcag cgtgggagat gctgtcacaa aagccggatt tagtgtttgt
    22321 cgatggtcat gggatctcgc atcctcgccg tcttggcgtc gccagccatt ttggtttgct
    22381 ggtggatgtg ccgaccattg gcgtagcgaa aaaacgacta tgcggtaaat tcgaaccgct
    22441 ctccagcgaa ccgggcgcgc tggccccact gatggataaa ggcgagcagc tggcctgggt
    22501 ctggcgcagc aaagcgcgct gtaacccgct atttatcgct actggtcatc gggtcagcgt
    22561 ggacagcgcg ctggcgtggg tacaacgctg catgaaaggc tatcgtctgc cggagccaac
    22621 gcgctgggcg gacgcggtgg cctcggaacg tccggcgttc gtgcgctata cagcaaatca
    22681 gccctaattc aggtaaactg cggataattt ccgtatttga gaactcaaca tgttacaaaa
    22741 cccaattcat ctgcgtctgg aacgcctgga aagctggcag catgtcactt tcatggcttg
    22801 cttatgcgaa cgcatgtacc ccaattacgc catgttctgc cagcaaaccg gttttggtga
    22861 tgggcaaatt taccgtcgta ttctcgatct catctgggaa acgctgaccg ttaaagatgc
    22921 aaaagtaaat ttcgacagcc aactggagaa atttgaagaa gcgattcctt cagccgacga
    22981 tttcgatctg tacggcgttt atccggcaat cgatgcctgc gtggcgttaa gtgaactggt
    23041 ccattcgcgt ttgagtggtg aaacgctcga acacgcggtg gaagtgagta agacctccat
    23101 cacgaccgtt gcgatgctgg aaatgactca ggctggtcgc gaaatgagcg atgaagagct
    23161 caaagaaaac ccagctgtag agcaagaatg ggacattcag tgggaaatat tccgactttt
    23221 agctgagtgc gaagaacgcg atatcgagct gataaaaggc cttagggcag acctgcgtga
    23281 ggcggatgag agcaatattg gtataatttt tcagcaataa gaccagaaaa cgtgatttaa
    23341 cgcctgattt gtcgtacctg gagtcttccc tttcgccccc cgtctggtct acatttgggg
    23401 ggcgaaaaaa agtggctatc ggtgcgtgta tgcaggagag tgctattctg gcatttccgt
    23461 cgcactcgat gcttagcaag cgataaacac attgtaagga taacttatga acaagactca
    23521 actgattgat gtaattgcag agaaagcaga actgtccaaa acccaggcta aagctgctct
    23581 ggagtccact ctggctgcaa ttactgagtc tctgaaagaa ggcgatgctg tacaactggt
    23641 tggtttcggt accttcaaag tgaaccaccg cgctgagcgt actggccgca acccgcagac
    23701 cggtaaagaa atcaaaattg ccgcagctaa cgtaccggca tttgtttctg gcaaggcact
    23761 gaaagacgca gttaagtaag attgcgtggc agtgaacagt tttaacgaag gggtggtttc
    23821 accccttttg tctttctggc gtcgatcatt gatgctggct ggcgctctgt ttctcactgc
    23881 ttgtagtcac aactcttcac ttcctccctt taccgccagt ggatttgctg aagaccaggg
    23941 cgcggtacgc atctggcgaa aagacagcgg cgataatgtg cattttcttg ccgtgtttag
    24001 cccgtggcgc agtggcgata ccacgacgcg agagtatcgc tggcagggcg ataacctcac
    24061 gctcatcaat atcaatgttt acagcaaacc cccggtgaat attcgcgcgc gttttgacga
    24121 tcgcggtgat ctgagcttta tgcaacgtga atccgatggg gaaaagcagc agctttctaa
    24181 cgaccaaatc gatttatacc gttatcgggc tgatcagatc cgccagatta gcgatgcctt
    24241 acgtcagggg agagtcgtgc tgcgccaggg gcgctggcat gcgatggaac agaccgtgac
    24301 cacctgcgaa gggcaaacca ttaaacctga tttagattcg caggcaatag cgcatatcga
    24361 gcgccgccag agccgctctt ctgttgatgt cagcgtggca tggctggaag cgcccgaagg
    24421 ttcgcaatta ctgttagtgg caaactctga tttctgtcgc tggcaaccca acgagaaaac
    24481 gttctgattt accagtggcc catacccata tgaccgccac caccgcagcc gccgtagccc
    24541 atgcccattc cggctccgcg cggaataccc gcttcagcca tcgcaatatc ccgtttcacc
    24601 cgtaactcat ctaacgactg acgcaaattc tccatctctt tggcgaccgc gttaattttg
    24661 ctgctatccg gtgggttcgc ggctaacagg gcattgtatt cataacgctt cgtcaccagt
    24721 tgctgttgca gtgcgctgct ttgagcgtaa aagtcattat ggattttctg ccacgctgtc
    24781 tgttgttcgc tggtcaaagg cgcggcattt tgctgccaca taccgtgtcc gccgtgagca
    24841 aatgcagatg tcgatcccat cgccattgct gaaagcgcca tcattaccag ggcaattttc
    24901 gtgttccgtt tcatggttaa tcctccagtg gttgtcttac ttcgggtatt gcatcttccg
    24961 tgccaacgat gaaacgctga tatgacgggt aatctggcat gataaacgag taaaaatgac
    25021 tcgcctgctg cgggtagcga gtcattttta ctcattgaaa cttaagcctt tgtgttacag
    25081 cgcagggtaa gcgctgataa aagatggcat gatttctgct gtcagaaagg gatgagcagg
    25141 caaagaagaa gatgcgtttt atgcaacgtt ctaaagactc cttagctaaa tggttaagcg
    25201 cgatcctccc cgtggtcatt gttgggctgg tagggttgtt tgcggtgacg gtgattcgcg
    25261 attatggccg cgagactgcc gccgccagac aaacgctgct ggaaaaaggc agtgtactta
    25321 ttcgtgctct cgaatccggc tcgcgcgtcg gcatggggat gcgcatgcat catgcgcagc
    25381 agcaggcctt actggaagaa atggccgggc agcctggagt acgttggttt gcggtcacgg
    25441 atgaacaagg aacaatcgtg atgcatagca actccggcat ggtgggaaaa cagctttatt
    25501 ccccgcagga aatgcagcag ttacatccgg gagatgaaga agtgtggcgg cggatcgata
    25561 gcgcagacgg cgagcctgtt ctggaaattt atcgccagtt tcaaccgatg tttgctgctg
    25621 gaatgcaccg gatgcgccat atgcagcagt atgccgcgac accacaagca attttcatcg
    25681 ctttcgacgc cagtaatatt gtgagtgccg aagatcgtga gcagagaaac accctgatta
    25741 tcctcttcgc cctggcgacg gtcttgctgg caagcgtatt gtcattcttc tggtatcgcc
    25801 gctatctgcg ctcgcgccag cttctacaag atgaaatgaa gcgcaaagag aagctggtgg
    25861 cgctggggca tcttgcggca ggcgttgccc acgaaattcg taacccgctt tcctcgatta
    25921 aagggctggc aaaatacttt gccgagcgcg cgccagcagg gggagaagcg catcaactgg
    25981 cgcaggtgat ggcgaaagaa gctgaccgtt taaaccgcgt ggtaagcgag ctgctggaac
    26041 tggttaagcc aacgcatctg gctttgcagg cggtggatct caacacgctg attaaccact
    26101 cattacagct ggtaagccag gatgcaaact gccgggagat ccagttacgc tttaccgcca
    26161 acgacacgtt gccggaaatt caggccgatc cggacaggct gactcaggtc ctgttgaatc
    26221 tttatctcaa tgctattcag gcgattggtc agcatggcgt gattagcgtg acggccagcg
    26281 aaagcggcgc gggcgtgaaa atcagcgtta ccgacagcgg taagggaatt gcggcagatc
    26341 agcttgaagc catcttcacc ccgtacttca ccaccaaagc cgaaggcacc ggactggggc
    26401 tggcggtcgt gcataatatt gttgaacaac acggtggtac aattcaggtc gcaagcctgg
    26461 agggaaaagg cgcaaggttc accctctggc ttccggtcaa tattacgcgt aaggacccac
    26521 aaggatgacg cacgataata tcgatattct ggtggtggat gatgacatta gccactgcac
    26581 tattttgcag gctttgctgc gcggctgggg ctataacgtc gcgctggcga acagcgggcg
    26641 acaggcgctg gagcaggtgc gggagcgggt ttttgatctt gtgctttgcg atgtgcgaat
    26701 ggcggagatg gacggcattg ccacgctaaa agagatcaaa gcgttaaatc cggcgattcc
    26761 ggtactgatt atgaccgcat actctagcgt cgagacggcg gtagaggcgt tgaaaaccgg
    26821 ggcgctggat tatctcatca agccgctgga tttcgataac ctgcaggcga cgctggaaaa
    26881 ggcgctcgcg catacgcaca ttattgatgc tgaaacgcct gcggtgactg ccagccagtt
    26941 cggtatggtc ggtaaaagcc cggcgatgca acacctgctc agtgaaatcg ccctcgtcgc
    27001 gccatcggaa gccacggtgc tgatccacgg cgattccggc accggtaaag agctggtcgc
    27061 cagggcgatt cacgccagta gcgcacgtag cgaaaaaccg ctggtaacgc tcaactgtgc
    27121 ggcactcaac gaatccttgc tggaatctga attgttcggt cacgaaaaag gggcgtttac
    27181 tggagccgat aaacgccggg aagggcgctt tgttgaggcg gacggcggca cgttgtttct
    27241 cgatgaaatt ggcgatatct cgccgatgat gctggtgcgt ctgctgcgtg cgattcagga
    27301 gcgcgaagtt cagcgtgtcg gtagcaacca gactatttcg gttgatgtcc ggctgattgc
    27361 cgcgacccat cgcgatcttg ccgcagaggt gaatgccggg cgttttcgcc aggatctcta
    27421 ctatcgcctg aacgtggtgg cgattgaagt accatcgctg cggcaacggc gggaagatat
    27481 tcctctgctg gctggccatt ttctgcagcg ctttgctgaa cgtaatcgca aggcggtaaa
    27541 aggctttacg ccccaggcga tggatctgtt gattcattac gactggccgg gaaatattcg
    27601 tgaactggaa aatgcggtgg aacgggcggt ggtgctgctg actggggaat atatttccga
    27661 acgcgagctg ccgctggcga ttgccagtac gccgatcccg ctggcacaaa gcctggatat
    27721 tcagccgctg gtggaagtcg aaaaagaagt gattctggcg gcactggaga aaacgggcgg
    27781 caacaaaacc gaagccgccc gtcagttagg gatcacgcgc aaaacgctgt tggcaaaatt
    27841 gtcgcgttag ttctgctcgc gttcgatagc gcgccagccg atatctttcc ggcagaagca
    27901 gtcgtcccag tggatatcgg tcattaaggc ataggcgcgt ttctgtgctt ctgctacggt
    27961 atgacccagc gcggtgacgc acagtacgcg cccgccgctg gttaccacct gctcgtcatc
    28021 cgccagtttt gtgcccgcgt ggaacacttt gccgtcttcc acttcttcca gcggcaggcc
    28081 gtggatcacg tcaccggtgc ggtaatcgcc cggatatcca cccgcagcca tcaccacgcc
    28141 gagagaagca cgttcatccc actctgacgt tttctcgtct agtttgcctt cacaggccgc
    28201 caggcagagt tcaacaagat cggacttcat gcgcagcata atcggctggg tttctggatc
    28261 gccaaagcgg cagttaaatt cgataacctt cggattgccc tgtttgtcga tcatcaggcc
    28321 cgcgtagaga aaaccggtgt aggtgttgcc ttccgacgcc atgcctttca cggttggcca
    28381 gatgatacgt tccatcgtgc gctgatgaac gtcatctgtc actaccggcg caggagagta
    28441 agcgcccatt ccgcccgtgt tcggcccggt atctttatcg cctacgcgtt tgtgatcctg
    28501 gctggtggcc atcggcagca catgctcgcc gtccaccatc acgataaagc tcgcttcttc
    28561 gccgtcgagg aactcttcga taacgatgcg atgacccgcg tcgccaaacg cgttgcccgc
    28621 cagcatatcg tgaacagccg cttccgcttc ttccagcgtc atcgccacga taacgccttt
    28681 cccggcagcc agaccgtccg ctttaatgac gattggcgcg cctttctcac gcagatacgc
    28741 cagcgcaggt tctacctcgg tgaagttctg atattccgcc gtaggaatgt tatggcgggc
    28801 caggaaatct ttagtaaacg ctttcgagcc ttccagctgt gcggcgcctg cggttgggcc
    28861 gaagattttc atcccggcgg cgcggaaggt atcgaccacg cctttcacca gcggcgcttc
    28921 cgggccgacg atggttagat ctaccttttc gttttgcgcg aaatccagca gcgccgggat
    28981 atcggtcacg ccgatagcga cgttttgcag cgtgggttcc agcgctgtgc ctgcgttacc
    29041 cggagcaaca aaaacagtct caaccagcgg cgactgggcc gctttccagg ccagcgcgtg
    29101 ctcgcgcccg ccgttaccaa tcactaatac tttcatctgt cgctccatta atggcggaag
    29161 tggcgcatat cggtgaagag catcgcaata ccgtgctcgt cggcggcggc aatcacttcg
    29221 tcatcacgga tggaaccgcc aggctggatt acgcaggtca cgcccgcagc ggcggcggca
    29281 tcaataccgt cgcggaacgg gaagaacgcg tcagaagcca tcgaggaacc tttcacttcc
    29341 aggccttcat cggcagcttt aataccggcg attttcgcgg agtacacgcg gctcatctgg
    29401 cccgcgccaa taccgatggt catattgttt ttggcataga cgatagcatt ggatttcacg
    29461 aacttcgcca ctttccagca gaacagcgca tcacgcagtt cctgttcggt cggctgacgt
    29521 ttggtgacga cgcgcagttc ttccgcaccg accatgccca gatcgcgatc ctgaaccagc
    29581 agaccgccgt tcacgcgttt gaaatcaaga cccggaacac gctcgcccca ctgaccgcag
    29641 gtcagaacgc gtacattctg tttggcggcg gtgattttca gggcttcttc gctggcggac
    29701 ggcgcaataa tcacttcaac aaactgacga gaaatgatgg cctgtgcggt ttccgcatcc
    29761 agctcgcggt taaaggcaat gatgccgccg aatgcggagg tcgggtcggt tttgtacgcg
    29821 cgatcgtaag catcaagaat tgaattgccg atagccacgc cgcaagggtt ggcgtgcttc
    29881 acaatcacac atgccggctc ggcgaactct ttcacgcact ccagcgccgc atcggtatcg
    29941 gcgatgttgt tataagagag ggctttaccc tgaacctggg ttgcggtagc aacggaggct
    30001 tctttcacat tctcttctat atagaaggca gcctgctggt ggctgttctc gccgtaacgc
    30061 atatcctgct tcttaatgaa gttcaggttc agcgtgcgtg ggaagcgacc ggcggcttct
    30121 ttgctttcac cgtgataagc cggaaccatg ctgccgaagt agttggcaat catgctgtcg
    30181 taggcggcag tgtgttcgaa ggctttgatg gcgaggtcga aacgggttgc aagcgtcagc
    30241 gatccttcgt tgtcatccat ctctttaata atggcgtcgt agtcgctgct cttcaccacg
    30301 attgcgacat ctttatggtt cttggcggcg gagcgcacca tcgttgggcc gccgatatcg
    30361 atgttctcaa ccgcatcttc cagcgagcaa ccttcacggg ccacggtctg ggcgaacgga
    30421 tacaggttaa caaccaccat atcgataggc tggatctgat gttcttccat aatggcatcg
    30481 tcctggccgc gacggcccag aatgccacca tgtactttcg gatgcagggt cttcacgcgt
    30541 ccatccatca tctccgggaa accggtgtaa tcggaaactt cggttaccgg cagacctttt
    30601 tctgctaaca gacgggcagt gccccctgta gacagcagct ccacaccgcg tgcggaaagt
    30661 gcctgggcga attcgacgat accggctttg tcagaaacac tgagcagagc gcggcggact
    30721 ggacgacgtt gttgcatggt aaatcccctg gatttgacta ttacagagag cgttagctga
    30781 atttttcgcg aaaaactcag ctaacgcccc taacggggca tccttatttt tcgcccgcat
    30841 tgtaacgaaa acgtttgcgc aacgctcgcg aattttttta attcgatggc gatcacgatt
    30901 ttgcccccga ttagcgtggg agaaatagag aaataacgct gatgattgtg gataactctg
    30961 tgcgtaaaaa ggtataaagc ggggttttgc tggggaatgc agcagtcagt catttttctg
    31021 caatttttct attgcggcct gcggagaact ccctataatg cgcctccatc gacacggcgg
    31081 atgtgaatca cttcacacaa acagccggtt cggttgaaga gaaaaattct g
//
LOCUS       DBFIFI010000039        30126 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.039, whole genome shotgun sequence.
ACCESSION   DBFIFI010000039 DBFIFI010000000
VERSION     DBFIFI010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30126
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.039"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            130..1728
                     /gene="rtcR"
                     /locus_tag="N7B39_001655"
     CDS             130..1728
                     /gene="rtcR"
                     /locus_tag="N7B39_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HGU2421658.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCICQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVSVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSLYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVADGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHASLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFASSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(1710..2468)
                     /locus_tag="N7B39_001656"
     CDS             complement(1710..2468)
                     /locus_tag="N7B39_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HGU2421659.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDTPPPVSVMQVLTDHHIQLELC"
     gene            complement(2485..3315)
                     /gene="glpG"
                     /locus_tag="N7B39_001657"
     CDS             complement(2485..3315)
                     /gene="glpG"
                     /locus_tag="N7B39_001657"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HGU2421660.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPALKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(3360..3686)
                     /gene="glpE"
                     /locus_tag="N7B39_001658"
     CDS             complement(3360..3686)
                     /gene="glpE"
                     /locus_tag="N7B39_001658"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HGU2421661.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRNNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            3876..5381
                     /gene="glpD"
                     /locus_tag="N7B39_001659"
     CDS             3876..5381
                     /gene="glpD"
                     /locus_tag="N7B39_001659"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HGU2421662.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAVSDLGEDFGHEFYETE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(5596..6198)
                     /locus_tag="N7B39_001660"
     CDS             complement(5596..6198)
                     /locus_tag="N7B39_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2421663.1"
                     /translation="MMNIIRLYIYPLFLGFVIVPLLVWPTVVALAACVITLSFLGEIL
                     FSIPLMHRRISLLQLQLWLSAEYALFFCAMVGVGWQFARRTPPELKSRLHCWLVFAPV
                     YFWLLLWNIIFYVAPAQIALLENMRSFFLTVVWLPLNFSPFWSQQWIDFVGPISAQLG
                     FALGYYCQWRSKNRSQIRKWSEWGTCLSFVTLGMMAWALR"
     gene            complement(6198..7190)
                     /locus_tag="N7B39_001661"
     CDS             complement(6198..7190)
                     /locus_tag="N7B39_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HGU2421664.1"
                     /translation="MNKFTGVLLLGTALLAGCVDREGYYNSVREEESHGLTSLRGQPA
                     LRYSDDWSRWPRVYGATALYPLYASAYYKLVPEPKDKDRTSLAWQAYGLQQTRTAEAY
                     DSLIKGSATVIFVAQPSEGQKKRAEEAGVKLKYTAFAREAFVFIVDINNPVNSLSEHQ
                     VKDIFSGKTSRWNKVGGSDEHIKVWQRPEDSGSQTIMKGLVMQDTPMLPAKKSTVIDL
                     MGGLITEVADYQNTPSSIGYTFHYYVTRMNDNMLKMRKQIKLLAINGVAPTEENIRNG
                     TYPYIVDAYMVTRENPTPETQKFVDWFISQQGQQLVEDVGYVPLYEASPESSGQ"
     gene            complement(7216..8724)
                     /locus_tag="N7B39_001662"
     CDS             complement(7216..8724)
                     /locus_tag="N7B39_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HGU2421665.1"
                     /translation="MMPNRKWILTSLIMTFFGIPILAQFLAAVVAMLGVGLTGIIEVC
                     NILITPTIYLLLNVFMLTLGAIIIFFSGRVWAGDSAPENREIAVWRQCFFLLPALLTL
                     VGWIITLHLADYQFRQMGAGWLANLMLPWLGVFLVSLVGGEYWWMVIIPVGAHISFSL
                     GYAWPTRYPLSGTSGLRCRNLLLFLLLLLGIVAGYQAHLYKQQNPGVGVRENIDIRAW
                     RPDKLNNRLTPLRGKPQIQFRQNWPRIDGATAAYPIYASAFYALSVIPEDFHVWEYLE
                     NSRTPDAYNRIVKGDADIIFVAQPSGGQKKRAEESGVTLLYTPFAREAFVFIVNADNP
                     VNSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKKVRMISP
                     QETEVASVMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIRLLAINGITPTAENI
                     RNGKYAYIVDAFMVTRENTTSETQKLVEWFLTPQGQSLVEDVGYVPLYLTME"
     gene            complement(8849..11296)
                     /gene="glgP"
                     /locus_tag="N7B39_001663"
     CDS             complement(8849..11296)
                     /gene="glgP"
                     /locus_tag="N7B39_001663"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HGU2421666.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADLDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(11315..12748)
                     /gene="glgA"
                     /locus_tag="N7B39_001664"
     CDS             complement(11315..12748)
                     /gene="glgA"
                     /locus_tag="N7B39_001664"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HGU2421667.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(12748..14043)
                     /gene="glgC"
                     /locus_tag="N7B39_001665"
     CDS             complement(12748..14043)
                     /gene="glgC"
                     /locus_tag="N7B39_001665"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HGU2421668.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(14061..16034)
                     /gene="glgX"
                     /locus_tag="N7B39_001666"
     CDS             complement(14061..16034)
                     /gene="glgX"
                     /locus_tag="N7B39_001666"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HGU2421669.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALHEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGVGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(16031..18217)
                     /gene="glgB"
                     /locus_tag="N7B39_001667"
     CDS             complement(16031..18217)
                     /gene="glgB"
                     /locus_tag="N7B39_001667"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HGU2421670.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPLNEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(18490..19593)
                     /gene="asd"
                     /locus_tag="N7B39_001668"
     CDS             complement(18490..19593)
                     /gene="asd"
                     /locus_tag="N7B39_001668"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HGU2421671.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            19785..20378
                     /gene="yhgN"
                     /locus_tag="N7B39_001669"
     CDS             19785..20378
                     /gene="yhgN"
                     /locus_tag="N7B39_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HGU2421672.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(20424..21719)
                     /locus_tag="N7B39_001670"
     CDS             complement(20424..21719)
                     /locus_tag="N7B39_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HGU2421673.1"
                     /translation="MDADSSNSTVTPGRLPDGLSSCPCFYLGKKLMNLSANAQATLLL
                     TSDFSRAAASKYKPLSNSEWGKFALWLKHQRISPAELLVPQPQEKLTGWSDPRISQER
                     ILGLLARGHSLALAVDKWQRAGLWILTRGDADYPVRLKNRLRTDAPPVLFGCGNKALL
                     QAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQGICVISGGARGIDECAMASALEAGGTA
                     VGVLADSLLKTSTLVKWREGLIAGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAM
                     VVRAGMTGGTITGAMEALKHQWLPVQVKPNQDMQSANSRLVENGASWSAEQAENVTIR
                     LPDVPGLMYDRALRNAQPELFSLHEDDANYAVMPAYTPVDFYQLFVAELAILAKESIS
                     IERLASCTGLTIEQISVWLNRAEEEGRVIRLGEGHYQFR"
     gene            complement(21626..23750)
                     /locus_tag="N7B39_001671"
                     /pseudo
     CDS             complement(21626..23750)
                     /locus_tag="N7B39_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
     gene            complement(23890..25230)
                     /gene="gntU"
                     /locus_tag="N7B39_001672"
     CDS             complement(23890..25230)
                     /gene="gntU"
                     /locus_tag="N7B39_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HGU2421674.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(25234..25761)
                     /gene="gntK"
                     /locus_tag="N7B39_001673"
     CDS             complement(25234..25761)
                     /gene="gntK"
                     /locus_tag="N7B39_001673"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HGU2421675.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(25900..26895)
                     /gene="gntR"
                     /locus_tag="N7B39_001674"
     CDS             complement(25900..26895)
                     /gene="gntR"
                     /locus_tag="N7B39_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HGU2421676.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(27119..27814)
                     /gene="yhhW"
                     /locus_tag="N7B39_001675"
     CDS             complement(27119..27814)
                     /gene="yhhW"
                     /locus_tag="N7B39_001675"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HGU2421677.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASISDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(27937..28974)
                     /gene="yhhX"
                     /locus_tag="N7B39_001676"
     CDS             complement(27937..28974)
                     /gene="yhhX"
                     /locus_tag="N7B39_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HGU2421678.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLNEVLNDPDVKLVVVCTHADSHFEYAKRAMEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            29308..29796
                     /gene="yhhY"
                     /locus_tag="N7B39_001677"
     CDS             29308..29796
                     /gene="yhhY"
                     /locus_tag="N7B39_001677"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HGU2421679.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYYNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDARWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYFMAR
                     VK"
     gene            30007..>30126
                     /locus_tag="N7B39_001678"
     CDS             30007..>30126
                     /locus_tag="N7B39_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2421680.1"
                     /translation="MGTAAISHLRYDLNKYALSLRKTATLASTFFIESPLVRFE"
BASE COUNT         7523 a         8091 c         7361 g         7151 t
ORIGIN      
        1 attaaataag cagattaatt ttttaattca ttgttttaaa aagattatat ctttacgtcc
       61 gtaaacggag atttcccgca aagccaattt accgataatg aaatatcgtc ttttataagg
      121 atatctaaga tgcgtaaaac agtggctttt ggctttgtcg gaacagtgct ggattatgcc
      181 gggcgcggca gtcagcgctg gtcgaaatgg cgtccgtcac tctgtatatg ccagcaagaa
      241 tcgttagtca tcgatcgact ggaattattg catgacgccc gctcgcgctc gctatttgaa
      301 acgcttaaac gcgatatcgc cagcgtttcg ccagaaaccg aagtggtgag cgttgagatt
      361 gaactgcata acccgtggga tttcgaagag gtttacgcct gcctgcatga tttcgcccgt
      421 ggttacgagt ttcagccaga aaaagaagac tatttaattc acatcaccac cggcacccac
      481 gtcgcgcaga tttgctggtt tctgctggct gaagcgcgtt acctgcccgc ccggttgata
      541 caatcttcac ctccgcgcaa aaaagaacag ccgcgcggcg caggtgaagt gacgattatc
      601 gatctcgatt taagccttta taacgccatc gccagccgct ttgccgagga acgccagcaa
      661 acgcttgatt ttcttaagtc cggtattgcc acgcgtaacc cccacttcaa ccgcatgatt
      721 gagcagattg aaaaagtagc gattaaatcc cgcgcaccga tcctgctaaa cggcccaacc
      781 ggtgcgggca agtcatttct ggcgcgacgt atatttgaat taaaacaggc gcggcatcag
      841 tttagcggcg cgtttgtgga agtgaactgc gcaaccctgc gcggcgatac cgccatgtcg
      901 acgctgtttg gtcatgtgaa aggcgcgttt accggggcac gggaatctcg tgaaggatta
      961 ttacgcagcg ccaacggcgg aatgttgttt cttgatgaga ttggcgaact gggcgcagac
     1021 gaacaggcaa tgctgctgaa agccattgaa gaaaaaacct tttatccgtt tggcagcgat
     1081 cgccaggtga gcagtgattt tcagcttatc gccggaacgg tgcgtgattt gcgccagctg
     1141 gttgccgatg gcaaattccg cgaagatcta tacgcgcgga tcaatctctg gaccttcacc
     1201 ctgccggggc tacgccagcg tcaggaagat attgaaccta acctggatta tgaagtggag
     1261 cgccacgctt cactcaccgg cgacagcgtg cgttttaaca ccgaagcgcg gcgcgcctgg
     1321 ctggcctttg cgacatctcc ccaggcaaca tggcgcggta actttcgcga gctttctgcc
     1381 agcgtcacgc gaatggcaac ctttgcctct agcggacgca ttactctgga cgtggttgaa
     1441 gatgagataa accgtctgcg ctataactgg caggagagcc gcccctccgc gcttacggcg
     1501 ttgctgggcg cagaggcaga aaacatcgat ctcttcgacc gtatgcaact ggaacacgtt
     1561 atcgcaatct gccgccaggc aaagtcgctt tccgcagccg gacgccagct ttttgacgtt
     1621 tcgcgccagg gcaaagccag cgtcaatgac gcggatcggc tacgcaaata cctggcgcgt
     1681 tttggtctga cgtgggaagc cgtgcaggat cagcacagct ccagttgaat atggtggtcc
     1741 gtcagcacct gcatcacgct tactggcggc ggggtgtcgg tgtagacggc atctaccatg
     1801 ctgatgctac ccatattgac catcgcgtta cggccaaatt tcgagtgatc gacaaccagc
     1861 ataacgtggc gcgagttctc aataatggcg cgtttggtgc gaacttcgtg gtaatcgaac
     1921 tccagcagcg aaccgtcgct atcgatgccg cttatcccca gaatgccgaa atcaaggcgg
     1981 aactgggaga taaaatcgag cgtcgcttcg ccaatgatcc cgccatcgcg gctgcgtaat
     2041 tcgccaccgg cgagaataat acggaaatcc tcttttacca tcagcgtgtt agcaacgtta
     2101 agattgttgg tgacaatgcg caaattgctg tgattgagca gcgcgtgcgc caccgcttcc
     2161 ggcgtggtgc cgatatcgat aaacagcgtc gagccattgg ggatttgctc cgccactttg
     2221 cgggcgatgc gctctttttc ttcggtctgg gtggctttac gatcgtgcca cggcgtgtta
     2281 accgaactgg aaggcagcgc cgcaccgcca tgatggcgca ggatcaggtt ttgctccgcc
     2341 agctcattga ggtcgcggcg aatagtctgc gggctgacgg agaagtgctc caccagctcc
     2401 tcggtactga cataaccctg ctgtttaacc agttcgataa taccgttgtg acgttgtgtt
     2461 tgtttcattt ctaaatccct ggaattattt tcgttttcgc gcattgagcg aatcaacaaa
     2521 agccatcgct aaacccacgg ctaacccggc gatgtgtgct ccgttcgcca tcgacatccc
     2581 aaacaaatca aaccatccgg cgacaatcca gatcagcgca aagataatta acccacgttg
     2641 caggtaaatg ccactttgcg gatcgcgttc gccacgtagc cagacgtagc ccatcagcgc
     2701 atacaccacg ccagaaagcc cgccaaacca cggcccgctg aatttttgct gcacatagcc
     2761 gcttaacagg gcgctgataa gcgtaatgac aattagctta ccgctaccga ggcgtttttc
     2821 caccgcaccg ccgagatacc accaccagag caggttaaag aggatatgca tcagcgagaa
     2881 gtgcattaac gcgtgggtga agtaacgcca gaactcaaat ttcagtgctg gatcgaatgg
     2941 ccaagccagc cataacatca cttcctgatc gccgagaatt tgcatggcaa taaacaccac
     3001 cacgcaggcg atcatcatca cccaggtcac cggacctgcg cgttcacgca aggcggcaaa
     3061 gaaaggataa cggcgataat gcaggccact gccggtatgg cctgcctgcc agctcgccgc
     3121 cagataacgc ggatctgccg ggttttcgag aaaacgcgcc agctccgccc gtacgcgctc
     3181 ggcctgggac tcatccgcca gccagacatc gctttggtta tgttgttgaa tcgtgaggat
     3241 aacaccctgc gtcgccatgt aatcaacaaa cgcctgcgcc acgcgggggt tagcaaaaga
     3301 ggtaatcatc aacatcgttg ctgtcgctta ttccacacaa aaggggacag tataaagcgt
     3361 tacgcgccgt acgccacctc tgcgggaaac tgacgttgcc aggcttcaaa gccgccgtca
     3421 atgctataga ccacatcgta gccctgttgc agcagatact gcgccgcgcc tttgctgcta
     3481 ttgccgtgat aacacatcac catcaccgga gtgtcaaagt cgttattacg cataaaagcg
     3541 cccagcgtgt cgttggttaa atggaaagcc tgcaccgcat gtcccattgc gaaactctgt
     3601 ggatcgcgaa tatcgaccag caccgcctct ttttcctgca acttctggtg cgcgtcggca
     3661 acgttaatac attcgaactg atccatgcgt ctctctttct ttacaaacaa gtgggcaaat
     3721 ttaccgcaca gtttacgtcg aagcggcaga taaacgccat aatgttatac atatcactct
     3781 aaaatgtttt ttcaatgtta cctaaagcgc gattctttgc taatatgttc gataacgaac
     3841 atttatgaac tttaacgaaa gtgaatgagg gcagcatgga aaccaaagat ctgattgtga
     3901 taggtggcgg catcaatggt gctggtatcg cggcagacgc cgctggacgc ggtttatccg
     3961 tgctgatgct ggaggcgcag gatctcgctt gcgcgacctc ttccgccagt tcaaaactca
     4021 ttcacggtgg cctgcgctac cttgagcact atgaattccg cctggtcagc gaggcgctgg
     4081 ctgaacgtga agtgctgctg aaaatggccc cgcatatcgc cttcccgatg cgttttcgcc
     4141 tgccacatcg tccgcatctg cgcccggcgt ggatgattcg cattggtctg tttatgtacg
     4201 atcatctggg taaacgcacc agcctgccgg gatcaactgg tttgcgtttt ggcgcaaatt
     4261 cagtgttaaa accggaaatt aagcgcggat tcgaatattc cgactgttgg gtagacgatg
     4321 cccgtctggt gctcgccaac gcccagatgg tggtgcgtaa aggcggcgaa gtgcttactc
     4381 gtactcgtgc tacttctgct cgtcgcgaaa acggcctgtg gattgtggaa gcggaagata
     4441 tcgataccgg caaaaaatat agctggcaag cgcgcggcct ggttaacgcc accggcccgt
     4501 gggtgaaaca gttcttcgac gacgggatgc atctgccttc gccttatggc attcgcctga
     4561 tcaaaggcag ccatattgtg gtgccgcgcg tgcataccca aaagcaggcc tacattctgc
     4621 aaaacgaaga taaacgtatt gtgttcgtga tcccgtggat ggacgagttt tccatcatcg
     4681 gcaccaccga tgtggaatac aaaggcgatc cgaaagcagt gaagattgaa gagagtgaaa
     4741 tcaattacct gctgaaagtg tataacgcgc actttaaaaa acagcttagc cgtgacgata
     4801 tcgtctggac ctactccggt gtgcgtccgc tgtgtgatga tgagtccgac tcgccgcagg
     4861 ctattacccg tgattacacc cttgatattc atgatgaaaa tggcaaagca ccgctgctgt
     4921 cggtattcgg cggtaagctg accacctacc gaaaactggc ggaacatgcg ctggaaaaac
     4981 taacgccgta ttatcagggt attggcccgg catggacgaa agagagcgtg ctaccgggtg
     5041 gcgccatcga gggcgaccgc gacgattatg ccgctcgctt acgccgccgt tatccgttcc
     5101 tgaccgaatc gctggcacgt cattacgcgc gtacttatgg cagcaacagc gagctgctgc
     5161 tcggcaatgc gggagcggta agcgatcttg gggaagattt cggtcatgag ttctacgaaa
     5221 cggagctgaa atatctggtc gaccacgagt gggtccgccg cgccgacgat gccctgtggc
     5281 gtcgcacaaa acaaggcatg tggctgaatg cggatcaaca atctcgtgtg agtcagtggc
     5341 tggtggaata tacgcagcag aagttgtcgc tggcgtcgta aattaacgta aggtgatcag
     5401 gtcagatttc aatctggcct gagactgatg acaaacacaa aattgcctga tgcgctacgc
     5461 ttatcaggcc tacgtggttt atgcaatatg ttgaatttgc atgattttgt aggcctgata
     5521 aggcgttcac gccgcatcag gcatgaacaa agcgccacgt tgtcaacaat ctgacctact
     5581 ttttgattca gggaattacc gcaaagccca cgccatcata cccaacgtaa caaaacttaa
     5641 gcacgttccc cactcactcc acttccgtat ctggcttcta tttttgctac gccactggca
     5701 ataatatccc aacgcaaaac caagctgtgc acttatcgga ccgacaaagt cgatccactg
     5761 ttgagaccaa aatggcgaaa aattcagtgg gagccagaca actgtcagaa agaaactgcg
     5821 catattttcc agcaaagcaa tttgcgctgg cgcgacataa aaaatgatat tccacaggag
     5881 caaccagaaa taaactggtg caaataccag ccaacaatgc agtctgcttt tcaattcagg
     5941 tggcgttctg cgcgcaaatt gccaaccaac gcccaccatt gcacagaaga aaagcgcata
     6001 ttcagccgat aaccatagtt gtaattgcag aagggaaata cgcctgtgca tgagcgggat
     6061 tgagaacagt atttctccca gaaaactcag cgtgattaca caggcagcca gcgcaaccac
     6121 cgtcggccac accaacaacg gtacaataac aaagccgaga aataacggat aaatataaag
     6181 tctgattata ttcatcatta ttgtcctgat gattcggggg atgcttcata cagcggcaca
     6241 taccccacat cctctaccaa ctgttgcccc tgctgactta taaaccagtc aacaaatttc
     6301 tgcgtttccg gcgtgggatt ttcacgcgtc accatatagg catccacaat gtatggataa
     6361 gtgccgttgc ggatattttc ctcggtaggc gcaacgccat ttatagccaa aagtttaatc
     6421 tgcttgcgca ttttgagcat attgtcattc atacgagtga cgtaatagtg gaaggtgtac
     6481 ccaatggaag atggcgtgtt ttgatagtcg gcaacttcag taattaaacc gcccataaga
     6541 tcaatcacag tggatttttt agctggcagc attggggtgt cttgcataac caaccccttc
     6601 ataatcgttt ggcttccaga atcttcaggg cgctgccaga cttttatatg ttcgtcacta
     6661 ccacctactt tattccagcg gctagttttg ccgctaaaaa tatctttaac ctggtgctca
     6721 gagagagaat ttaccgggtt attaatatca acgataaaga caaaggcttc gcgggcgaaa
     6781 gcggtatatt tcagtttaac acccgcttct tctgcacgtt ttttctgtcc ttccgacggt
     6841 tgtgcaacaa aaataaccgt cgcggaacct ttaatcagac tatcgtaggc ttcagctgtt
     6901 cgggtttgct gcaaaccata cgcctgccag gccagcgagg ttcgatcctt atcttttggc
     6961 tcgggtacta atttataata cgcggaggca tacagcgggt ataaggctgt agcgccgtac
     7021 actctcggcc atcttgacca atcatcgctg taacgtaatg caggttgccc ccgcagagac
     7081 gtcagtccat ggctctcttc ttccctgacg ctgttatagt acccttcccg gtcgacacat
     7141 cccgccagta acgccgtgcc taatagtaaa acaccggtga atttattcat ctcgctgatt
     7201 cctttctctc gagcgttatt ccattgttag atacagcggc acatatccca catcttctac
     7261 cagactctgc ccctgcggcg ttaaaaacca ttcgaccagt ttttgtgttt ctgacgttgt
     7321 attttctctc gtcaccataa atgcatcgac gatgtacgca tatttgccgt tgcgaatgtt
     7381 ttccgccgtc ggtgtaatac cgttaatcgc tagcaatctt atatttttat cagcattcat
     7441 ttgcgtcgcg taatagcgga aggtatagcc tattgcgttg tttgtattac ggtattcggc
     7501 aacgacttta atcattccct ccatcacgct tgccacttcc gtttcctgcg gcgagatcat
     7561 gcggactttt ttcatgacct gtgattgcat cactgtctgg ctgccagagt cttccgggcg
     7621 ctgccaggtc tggatctcct gatcgttacc gccaacagta cgccaattgg taattgcacc
     7681 actgaaaatg tcacgcacct gttgttcagt cagggaatta accggattat ccgcattgac
     7741 gatgaaaaca aatgcttcac gggcaaatgg cgtgtatagc aaagtgacgc ccgattcctc
     7801 agcgcgtttt ttctgcccgc cggagggttg cgccacaaaa ataatatcgg catcaccttt
     7861 aacaatccgg ttatatgcat cgggggtacg agagttctcc agatattccc aaacgtgaaa
     7921 atcctctggt attacactta atgcataaaa tgcagaagca taaattgggt acgcagccgt
     7981 ggcgccatcg attcgcggcc agttttgcct aaactgaatt tgcggtttgc cacgcagcgg
     8041 cgtcagtcga ttattgagtt tatcgggtcg ccaggccctg atatcaatat tttcgcgtac
     8101 accgacacca ggattttgct gcttatataa atgggcctga tacccggcga caataccaag
     8161 taagagaagt agaaacagga gtaagttacg gcaacgtagt ccggacgtgc cggataaagg
     8221 atatctggtc ggccaggcgt atcccagcga aaaactgata tgcgccccaa cgggaataat
     8281 gaccatccac cagtactcac cacccactaa tgagactaaa aaaacgccca accagggaag
     8341 cataaggttt gccaaccaac cagctcccat ctggcgaaat tgataatctg ccagatgtag
     8401 cgtgattatc cagccaacca gggttaatag cgcgggtaag agaaaaaagc attgccgcca
     8461 gacggctatt tctctgtttt ctggcgcgct atcaccggcc cacacgcgac cagagaaaaa
     8521 tattattatc gcccccagcg tcagcataaa gacgttgagt agaagataaa ttgttggcgt
     8581 gataaggata ttacaaactt caataatacc ggtaagcccg acacccagca tggcaacaac
     8641 cgctgccaaa aattgcgcca gtatggggat gccgaaaaaa gtcattatca gcgaggtcaa
     8701 aatccatttt ctatttggca tcattctgtc cattcttcct gaatgacgga attatactcc
     8761 ccgagaaccc tcgccccctc tggacacttt tccgaaataa aggcgaaaaa aacgggacct
     8821 tgtggccccg ttctgttttt tagcgaactt acaatctcac cggatcgata tgccagatat
     8881 gatcagcgta ctctttgata gtacggtcag aagagaagta gcccatattg gcaatgttca
     8941 gcatcgcttt tgcggtccac tcttcctgaa gctcgtagag ttcatcgact ttatcctgac
     9001 aatcgacata gctgcgataa tccgccagta cctggtagtg atcgccgaag ttgatcagcg
     9061 aatcaaccag atcgcgatag cgacccggat cttccggact gaatacaccg ctgccgattt
     9121 gcgtcagcac ctgatgcagc tcctcatctt tctcgtagta ttcacgcggt ttgtagccct
     9181 gacgacgcag ttcttccact tcttccgctg tgttaccaaa aataaagata ttgtcagcac
     9241 cgacatgatc cagcatctcg acattcgcac cgtccaacgt accgatagtc agcgcaccgt
     9301 taagcgcaaa cttcatgtta ctagtgccgg aagcttccgt ccctgccagc gaaatctgtt
     9361 cagacagatc tgccgccgga atgatcaact gcgccaggct aacgctgtag ttcgggatga
     9421 acacgacttt cagcttatcg ccaatctgcg gatcgttgtt gatcactttc gctacgtcat
     9481 tgatcaaatg gataatatgc ttcgccatgt aataggccga agccgcctta ccgccaaaaa
     9541 tattcacgcg cggtacccac ttcgcatcca ggtcggcctt gatgcggtta tagcgggtaa
     9601 tcacatgcaa cacattcatc aattgacgtt tgtattcgtg aatacgtttg atttgcacat
     9661 cgaacaacgc ctttggattc accaccacat tcagctgctg ggcgatatac tccgccagac
     9721 gctttttatt ctccagcttc gcctgatgca cagcgtgatt aaccattggg aaatcacagt
     9781 gttgttgcag ctcattaagc aggctaaggt cggtgcgcca gttacggccc aggtgttcgt
     9841 ccagcacggc tgaaagcgat gggttcgcta ccgccagcca gcgacgcggc gtcacaccgt
     9901 tggtgacgtt ggtgaaacga cccgggaaga ttttcgcaaa gtcggcaaac aacgattgca
     9961 ccatcagatt agagtgcagt tccgatacac cgttaacttt gtggctcaca acaaccgcca
    10021 gccaggccat acgcacacga cgaccgttgg attcatcaat gatcgacgcc cgtcccagca
    10081 gatcggtatc gttcggatac tgttcctgca aggttttcag gaaatagtcg ttgatttcaa
    10141 agatgatctg caggtgacgc ggcagaattt tacccagcat atcaaccggc caggtttcca
    10201 gcgcctcgct catcagcgtg tggttagtgt aggagaagac ctgacaacac acctcaaacg
    10261 cgtcgtccca gctaaattgg tgctcatcga tcagcagacg catcatctca ggaatcgaca
    10321 gtaccggatg ggtatcattg agatgaatcg cgattttatc cgccaggtta tcgtaggttt
    10381 tatgcaactg ataatggcgg cttaaaatgt cctgaatggt cgaggaaacc aggaagtatt
    10441 cctgacgcag gcgcagctca cgcccggagt aggtggagtc atccggatac agtacgcgag
    10501 atacgttctc ggagtggttt ttatcttcca ctgccgcgaa gtagtcaccc tggttgaatt
    10561 taccgaggtt aatttcgcta ctggcttgcg cactccacaa acgcagcgtg ttggtcgcgt
    10621 cggtgtcgta accagggatt atctgatcgt aagcgactcc cagaatctct tcggtttcaa
    10681 tccagcgcgt ttttttacct tcctgctgaa tgcgaccacc aaaacggact ttatagcgcg
    10741 tgttgtggcg tttgaattcc cacgggttac catattccag ccagtagtca ggcgactctt
    10801 tctggctacc gttaacgatg ttctgcttga acataccgta gtcatagcgg atgccgtaac
    10861 cgcgccccgg caaccctaac gtcgccagag aatcgaggaa gcaagccgcc agacgtccca
    10921 ggccaccgtt accgaggccc gggtcatttt cttcatcaat cagctcttcg agatttaacc
    10981 ccatcgcttc cagtgcgccc tgtacatctt cgtaaattcc tagcgacaac atggcgttgg
    11041 agagcgtacg gccaatcaaa aactccatcg acaggtagta aacctgacga gtttcttgcg
    11101 acaactgggc acggtttgaa cgtaaccagc gctccacgag acgatcgcgc acagcaaata
    11161 acgttgcgtt cagccattca tgtttattgg cgacgaccgg gtcctttcca atcgtaaaca
    11221 tcagcttgta agcgatagag tgcttaagag cttctacgct aagcgtgggc gatgaatatg
    11281 taaacggagc attcatatag gcgtttcctg aaaactattt caagcgatag taaagctcac
    11341 ggtacgactt cgccgcgacc tgccagctaa aatccattgc catagcctga cgttgcacaa
    11401 accgccacag agaaggacgg gaccacagta caaaagcacg tcgaatagcc cgtaacagcg
    11461 accaggcatt actatcttca aagacaaacc cactggcgac gccatctgca aggttctcaa
    11521 gagaacagtc agaaaccgta tcagcaagcc caccggtgcg ccgcactaac ggcagcgtac
    11581 cgtacttcaa tccataaagt tgcgttaagc cgcacggttc aaaacggctg ggcaccagaa
    11641 tgacgtccgc accgcccata atgcgatgcg aaaatgcttc gtgatagcca atctgaacgc
    11701 ccacctggcc ggggtattcc gctgccgccg caaggaaacc ttcctgcagc accggatcgc
    11761 ccgcgccgag tagcgccagc tgcccgccct gctccagaag acccggtaag gcctccagca
    11821 ccaggtcgag gcctttctgg ctggtcagac ggctcaccac tgcaaaaagc ggcactttat
    11881 cgtcaacctt aagccccatt gcgatttgta actggcgctt attttccgct ttatcttcca
    11941 acgtatcgcg ggtgtaacgc gaggccaata gtaagtccgt ctctggactc cagattttct
    12001 cgtccacgcc gttcagtacg ccggaaagac gcccttcacg atgacgctgt tgcaacaaac
    12061 cttccatacc gtaggcaaac tgcggttcgg tgatctcgcg agcgtaggtt ggactgaccg
    12121 ccgtaatgtg atcggcatag tacagaccgg ccttcaggaa agagatttgt ccgttgaatt
    12181 ccagcccatg aatattaaag aatgaccatg gcaattggat gtcattcatg tgatgtgcat
    12241 aaaacatgcc ttgataggcc aggttgtgca cagtaaacac cgacttcgcc ggacgcccgc
    12301 gcgccgccag atacgcaggc gcaaggcctg catgccagtc gtgcgcatgc accacatcag
    12361 gacgccagaa tgggtcaagc ccgctggcca tttctgcccc aacccacccc agcagcgcaa
    12421 aacgcaatac gttgtcggta taggcaaata agttggtatc gtgatacggg cttcccggac
    12481 gatcatagag atgcggcgcg tcaatcaggt atatgccaac cccgttgtaa tgaccgaaca
    12541 acagcgtgat atgcccggcg aaggtatcac gacgggatac tacctgcgca tcggtcacgc
    12601 cacggcgaat atcgggaaat gcaggcaaca gtacgcgagc gtcaacgccg tctgcgattt
    12661 gtgctgcggg taatgcccca ataacatcag ccagaccgcc ggttttaagc agcgggaaca
    12721 tctctgaaca tacatgtaaa acctgcatta tcgctcctgt ttatgcccta acttccgtag
    12781 catttcgcgc gttaccagca cgatgccttc ttctgaacga tagaaacgac gtgcatcttc
    12841 ctctgcgttt tcaccaatca ccatgccttc cggaataaca caagcacgat cgatgacgca
    12901 gcggcgcaga cggcacgagc gacctaccca tacttccggt aacaatacgg cggaatcaat
    12961 gttgcagaat gaattcacgc gaacgcgcga gaacagaacg gactgcacca ccaccgaacc
    13021 ggagatcaca caaccgccgg aaaccagtga gttaagggtc atcccgtggc taccggagcg
    13081 atcctgcacg aatttcgctg gcggtaatga ttcattgtag gtgcgaattg gccaattgcg
    13141 atcgtacata tccagttccg gcaccacaga ggccagatcg aggttcgctt tccagtaagc
    13201 ttccagcgta cccacatcgc gccagtacgg ctcggcatcc ggatcggatt gtacgcaaga
    13261 gagcgggaac gggtgcgcat aggccagacc ggcttcggtg atcttgggaa tcaaatcttt
    13321 gccaaagtcg tggctggagt tctcatcgcg atcgtcttct tccagcagtt catacagata
    13381 gtcggcgtca aagacgtaga tacccatact cgccagagat ttgctcggat cgttcggcat
    13441 tgacggcggg ttagcaggtt tttcaacgaa ttcgataatt ttatcgttct catcaaccgc
    13501 cataacgcca aatgcggagg cttcttcaat cggtactggc atacaagcaa cggtgcaacg
    13561 tgcgcctttt tcgacgtgat cgataagcat acgcgagtag tcttgcttgt agatatggtc
    13621 gcccgccagg atcaccacgt attccgcttt ataacggcga ataatatcga ggttttgggt
    13681 gaccgcatct gcggtgccgc gataccagtt ttcccctttc attctctgct gtgctggcag
    13741 cagatcgaca aactcgttca tttcttcatt gaagaatgac cagccgcgct gaatgtgctg
    13801 caccagagtg tgggactggt actgggtgat cacgcccata cgacggatcc cggagttgat
    13861 gcagttagac agcgcaaagt cgataatgcg gaacttaccg ccgaagtgta cggccggttt
    13921 tgctcgctta ttggttaaat ccttcaggcg ggtaccacgt cctcccgcca gtatcagggc
    13981 aacagatttc aatggcagct ggcgcgccaa cattaagtga tcgttcttct ctaaactaac
    14041 catgactaac tcctttttta tcatctctgg aacacacaca atccgtgtgc aggtccctgc
    14101 cagacagccg taatcactgg gttatcctct ccagcgaatg ggggaatggc gtgccactcc
    14161 ccagcaggta aaacaatctc tgttacctca agcgtggcgt taattgcgat caaaaagcga
    14221 tccgagagca gaatttgcag ctgtttcggc ccgttttgcc actcatccgt gcttaaaggt
    14281 tgagcatatc gatttagcca acggacattg ccgtcgcctt cttcccacca gcgattctcc
    14341 accaaagcgg gaatacgctt gcgcagatgg attaacgcgg cggtaaatgc ggttaaacca
    14401 ctgcttgcct gcgaccagtc caaccaggtt aattggttat cctgacagta ggcattgtta
    14461 ttgccatgct ggctgtgacc atgttcgtca ccggccagta acatcggcgt accctgggag
    14521 agcaacaacg ttgttaacag ggcgtgaatg ctgtcgcgcc gccgttcaac caggtcaagg
    14581 gtaccgccta ccccttcttt accatgattg ttactgtaat tgttgttggt cccgtcgcga
    14641 ttttcttctc cgtttgcttc attgtgttta tggttgaaac aaacgcagtc gcgaagcgta
    14701 aaaccgtcat gcgcggtgac gagattaatc gcggcactcg gcagacgacc attacgttta
    14761 aaaacatcgc tggaggcagc aaaacgcccg gcaaacgccc ccagaggcaa atcataatgt
    14821 agccagaaac gacgggcagc atcgcggaaa tgatcgttcc actcggcaaa cagcggcggg
    14881 aaatttccca cctgataacc accaggagcg atatcccacg gttcagcaat taacttcacc
    14941 tgcgagagca ccgggcagtt ctggatagcg gtaaacaacg gcgcatcctg acggaactct
    15001 ggcgtacggc ccatgactgc cgccagatca aagcggaaac catcgacgtg gcaggtttct
    15061 acccaataac gcaggcaggc gctggcataa tccaccaccg ccgggtgact caaattgagc
    15121 gtgttgccac aaccggtcca gttgtgataa tcgccgtctt ctcttatcca ataatagcta
    15181 cggttgtcga tcccacgcag cgagaataac gggccgtcga ggtccagttc cgcactatgg
    15241 ttgagcacga tatcaagaat gacttcgata cccgctttat gcagtgcttt gattgcatcg
    15301 cgaaactcat gcagcgccgt ttctggcgag caggcatacg ccggatgcag cgcaaacatc
    15361 gccaccgggt tgtaacccca gtagttactt agccccatgc gttgcagacg tggttcactg
    15421 gcaaactgcg ccactggcag cagttccagc gcggtaatgc ccaattgttt caaatagttg
    15481 atcatcaccg gatgcccgag ggctttataa gtgccacgga tctcgaccgg gatctccggg
    15541 tgcaggtacg ttaatccttt aacatgggct tcataaatga tggtgctgcc ccacggcgtg
    15601 cgcggcgggg catcatcttc ccagtcatag tgatcaacca ccactacgca tttcggcgca
    15661 atggcggcgt tgtcgcgata gtcaggttca ttatgaccgg cgtgcagcag cgggttatct
    15721 ttaaactccc cgtcaatttg ccgcgcgcaa ggatcaatca acaacttcgc cgggttaaag
    15781 cgatgcccct cggcgggttg ccaggggcca tgaacgcgat aaccataacg caaacccggg
    15841 cgcgcatccg gcagataacc gtgccaaatg tcgccactgt gccctggcaa gtcatagcga
    15901 tgttcctggc cattggcgtc aaagacacac agttctaccc gctcggcatg agcggagaaa
    15961 agtgtgaagt tgacgccctg accgtcgtaa tgcgcgccga ggggagcggg tttgccaatg
    16021 gcgagttgtg tcattctgcc tcccgaacca gccagatagt ggccagcggt ggtagcgtca
    16081 ggcttagtga atgctgacga ccgtggctgg caatctcatc gctgtgtacc gtgccgccat
    16141 tacctgcatt actgccgtga tagtgcatgg aatcggtatt gaggatctca cgccatttgc
    16201 ctggctgatt aatgccgaag cgataatcat gacgcggcac cggcgtaaag ttactggcaa
    16261 cgatgatttc gttaccctct ttatcgcgac gcacaaagat cagcaccgag cgttctttgt
    16321 catccaccac cagccattca aagccgtacg ggtcaaaatc cagttcatgc atcgctttat
    16381 ggtggcggta ggtgaggttc agatcgcgca ccagacgctg gacaccgtgg tgccagttat
    16441 cgccgccttc caacagatgc cagtcgaggc tggcgtcatg gttccactcg cggccctggg
    16501 caaattcgtt ccccatgaac agcagtttct tgcccggaaa tgcccacatc cagccatagt
    16561 aagcgcgcag gttagcgaat ttctgccatg cgtcgcccgg catgcggtcg agaatcgatt
    16621 ttttaccgtg gaccacttca tcatgcgaca acggcaggac gaagttttca gtgtagttgt
    16681 agagcatccc gaaggtcagt ttatcgtgat gatactgacg ataaatcggg tcgagcttca
    16741 tgtagtccag ggtgtcatgc atccagccga ggttccactt gtaccagaag cccagaccgc
    16801 ccatatcctg cggacgagaa acgccaggga aatcggtaga ctcctcagcc attgtcaccg
    16861 caccggaaac ctgctcacca agaatacggt tggtattacg caagaattca atcgcttcaa
    16921 gattctcgcg cccgccaaat tcgttcggga tccactcccc ctctttacgg ctgtagtcac
    16981 gataaatcat cgacgccacc gcatcgacgc gcagcgcatc aataccaaaa cgttcaatcc
    17041 agtaaagcgc gttaccgacg aggaagttac tgacttcacg gcgaccatag ttgtaaatca
    17101 gcgtgttcca gtcctgatga tagccttcgc gcggatcgct gtgttcatac aagttcgtac
    17161 catcaaattc ggcaagcgca aagtcatcag tcgggaagtg gcctggcacc cagtcgagaa
    17221 tcacgttcag accagctgcg tgtgcagcat caatgaaata acggaagtcg tcgcgagtac
    17281 caaaacggcg ggttggtgca tacaggccgg ttggctgata accccaactg ccatcgaagg
    17341 gatgctcgtt aaggggcagt agttcgaggt gggtaaagcc catccattta gcataaggca
    17401 ccagttgatc ggccagctcg cggtagctca accagaaatt gttgtcggtg tggcgacgcc
    17461 aggagcccag gtgaacttca taaatagaga ttggcgcatc aaactgattc gcttttttgc
    17521 gctcttcagt ctgtacaacc ttttccggca gcccgcaaat aagagacgcg gtttccgggc
    17581 gcatttgcgc ttcgaaggca taagggtcgg acttcagacg caagttgcca ttggcatcaa
    17641 tcatctcgta tttatagagc tgaccgttat gcgccccagg gataaacagt tcccagatgc
    17701 cgctctcttt acgcaggcgc atcgggtgac ggcgaccgtc ccagtagttg aattgcccaa
    17761 ccaccgagac ccgacgggcg tttggagccc agacagagaa acgcgtacct gtgacgccat
    17821 ccatagtatc tgcatgcgcg cctaaagttt catacgggcg caggtgagta ccttcagata
    17881 atagccaggc atccatttcc tggattagcg gaccaaaacg gtaaggatca tcaatcaggt
    17941 tttgctgccc atgccagaca acagccaact gatagcggaa aaaattctta cgtcgcggaa
    18001 tgacgccact aaagaatccc cgtgagtcga gacactccag ttttgcgagt ttgcgcccgg
    18061 ttttcggttc aatcacccac acatcggtag cgtcgggtaa aagggcacgg acttccagtc
    18121 ccgcggtggt tttatgcatt cccagtacgg aaaaaggatc cgcaaaatgg cctgcaatta
    18181 gcgcgttaat cacgtctcta tcgatacgat cggacatgct tgtcttcctg ttttattgtg
    18241 tcaccccatc caattggatt tttgacttct ggttgtgaca tttttttgac ctgaacggcg
    18301 cagcactctg tgcatcctct ctgcgtcgtc ctcacttcag gtaaggctgt gaatactcat
    18361 gtattcagcc acccttaaag aatagccaat gctctattta actcccggta aatcatgaaa
    18421 catctgcgct tactcctgta ttacgcacta acaggggcgg catcgcgccc cagatttaat
    18481 gaataaagat tacgccagtt gacgaagcat ccgacgcagc ggctccgcgg ccccccacag
    18541 cagctggtcg cccacggtaa aggctgacag gaactctggt cccatattca gcttacgcag
    18601 gcggcctacc ggcgtggtca gcgtgccggt aacggcagct ggggttagct cacgcatagt
    18661 gatttcccga tcgttcggaa cgactttcgc ccacggattg tgcgcagcca gcagttcttc
    18721 cacggtcgga atcgacacat cttttttcaa tttaatagtg aatgcctggc tgtggcagcg
    18781 caatgccccg acacgcacac ataaaccatc taccggaatt acggaagatg tgttgaggat
    18841 cttgttggtt tccgcctgcc ctttccactc ttcgcggctc tgaccgttat cgagctgttt
    18901 gtcgatccac ggaatcaggc tacccgccag cggcacgcca aagttatcca ccggcagctc
    18961 accgctacgg gttaaggttg tgactttgcg ttcgatatcg agaatagcag aggacggggt
    19021 cgcgagttca tctgctacat ggccatacag atggcccatc tgggttaata actcacgcat
    19081 atgccgcgca ccaccgccgg aagcggcctg gtaggttgca acggacaccc aatcaacaag
    19141 atcattggcg aataaaccac ccaacgacat caacatcagg cttacggtac agttaccgcc
    19201 aacaaaagtc ctgatgccat tatttaatcc gtcggtaatg acgtcctgat tgacggggtc
    19261 aagaatgatg atggcgtcat ctttcatgcg cagagacgat gctgcgtcaa tccagtaacc
    19321 ctgccatccg ctttcacgaa gctttggata gatttcgttg gtataatcgc cgccctgaca
    19381 ggtcacaatg atatcgaggg cctttagcgc ctccagatca aaggcatcct gaagtgtgcc
    19441 agtggttccg ccaaaagacg gcgcagcctg accaagctga gaagtagaaa agaagacagg
    19501 gcgaatggcg tcgaagtcgc gctcttcaac catgcgttgc atgagaacgg agccgaccat
    19561 accgcgccag ccgataaaac caacattttt cataagcgtt ttttcctgca aagatgtgtg
    19621 ctgtataaat gtgccggtct cctcttggca catctttcac catacaaaaa gcagccaaag
    19681 tcgcaagtga aattaatcaa tgatagcgaa gccatcagta atgcgactta tcctgctttg
    19741 ttagcacgca gaaagtccgc ggcaattatc agggaatttg agttatgaat gaaatcattt
    19801 ctgcagcagt tttattgatc ctgattatgg atccgctcgg aaacctacct attttcatgt
    19861 ccgtactgaa acatactgaa ccgaaaagac ggcgggcaat catggtgcga gagttgctta
    19921 ttgctctcct ggtgatgctg gtgttcctgt ttgcgggtga gaaaattctg gcatttctca
    19981 gcctgcgagc agaaaccgtc tccatttctg gcggcatcat tctgtttcta attgccatta
    20041 agatgatttt ccccagcgct tcaggaaata gcagcgggct tccggcaggt gaagagccat
    20101 ttatcgtgcc gctggcaatt ccgctggtcg ccgggccaac tattctcgcc acgctgatgt
    20161 tgttgtctca tcagtacccg aatcagatgg gacatctggt gattgctctg ctgctggcct
    20221 ggggcggcac ctttgtcatc ctgctacagt cttcgctatt tttacgtctg ctgggcgaga
    20281 aaggggtgaa cgcacttgaa cgcctgatgg gattgattct ggtgatgatg gcaacccaga
    20341 tgttcctcga cggcattcga atgtggatga aggggtaagg ttcaaacagg tgctgcacac
    20401 aaccagacgc ggcacctgtt ttattacctg aactgataat gaccttcgcc caatcggata
    20461 acccttccct cttcttctgc gcggttcagc cacacactaa tttgttcgat ggttaaacca
    20521 gtacaagacg ccagcctttc aatacttatc gattcctttg caaggatcgc cagttccgcc
    20581 acaaagagtt gataaaaatc gacaggcgta tacgcgggca ttactgcgta atttgcgtca
    20641 tcttcatgca gcgaaaacag ttctggttga gcgttacgga gtgctctgtc atacatcagc
    20701 ccaggaacgt ctggcagtct gatcgtcaca ttctcagcct gttcagcact ccatgacgcc
    20761 ccattttcta ctaatcgtga attggctgat tgcatatcct gatttggttt aacctgcaca
    20821 ggcagccact gatgttttaa tgcctccatc gccccggtta tcgttccacc ggtcattccc
    20881 gcacgtacaa ccattgcgct ttcagcaagg caataaatat atttatttcg cgccatcgca
    20941 ttgccgacgg tgaaacgtac ctctgggtaa aacggcgaaa tcaacaccag gttgcctgct
    21001 ataagccctt cacgccattt cactaacgta ctcgttttta acaagctatc agctaatacg
    21061 ccaacggcag ttcccccggc ctccagtgcc gacgccattg cacattcatc aatacctcgc
    21121 gcaccaccag agataacgca aatcccctgt tgggccagtt tcgcggccag ttgttgggta
    21181 tagcgcaaat cgtcagtcgg agcatctcgc gagccaacaa tcgccatacc ttccgcttgc
    21241 agtaatgctt tattcccgca gccaaataaa acgggaggtg catccgttcg caatcggttt
    21301 ttcaagcgaa cgggataatc agcatctccg cgggttaaga tccataaacc ggcgcgttgc
    21361 cacttatcta ccgccaacgc cagactatga ccacgcgcca gcaagccaag aatacgctcc
    21421 tgagaaatac gcggatcgct ccagcctgta agtttctctt gcggttgcgg caccagaagc
    21481 tcggcgggac tgatacgttg gtgcttcagc cataatgcaa acttccccca ttcactatta
    21541 ctaagaggtt tatacttact cgccgccgcg cgagaaaaat cgctggttag caggagagtt
    21601 gcttgtgcat tggctgaaag attcatcaat tttttaccga ggtagaagca agggcaagag
    21661 gataaaccgt cgggcaacct gcctggcgta acagtgctgt tagaactgtc agcgtccatg
    21721 cagaatccac gatatcgtca accagcaacg ccggaccagg catcaaagga ggggtaatca
    21781 caaacgcccc gtcgagattt tgacactggt ggaaacggtt ttgctgcatt ttctgcggtg
    21841 gattgtccac gactttttca atggcatcaa taaaaggtaa gccaagtttc gccgccagtc
    21901 gccgggcaaa atcaggaacc aggtcgaggt gctttcaaaa tcaggaacca ggtcgaggtg
    21961 ctttaatgaa ggaacgcagc atacccaggt tggttcagga tgcggattcc agcgttggcg
    22021 aaccatttcc gcacatgcct ccaccagttc atcactgaat cgccccgttt ttttctcctg
    22081 tgctacctgc tttccccacc cggaatatcc ccaacgagaa aggattcgcc ccgtagatcc
    22141 ttgtaaactc gcaggcaagt tccctttaaa tccgtattgc gtaaaagccc cagctgccac
    22201 ctgtttattg agatttaatg acaggtcagc gtgtttaata aataagtttg ccgcatggag
    22261 taagccgcta tcaatgtcgg gcgacaacaa cggatgctga agacaactgc tacatttacc
    22321 acaatgttcg gcacctggct catcgagtgc gcggcgcaga aattgcattt tgcactcctt
    22381 ggtcgtgata tagctttcaa cgtctgccag ctcacttttt ctctgattca tcaaatggtt
    22441 aattcgttca tggtcaggag aaaaactgac aattgttctg cgccataatt tctcggtata
    22501 caccacaggc gatggatttt cagctaccaa taatttcagt gctttttcta tttgcccata
    22561 acggagattc gtccgttgtt caatgcctcg taaggtaaga ccgtcattca cgctaagtac
    22621 gttgagtatt tcatgaattt gcgcctccgc aggaaaggcg ctttcacgga agaatttatg
    22681 aatagcacgg tcttcactgc cgcaaagcag tatgccaaca gcagaatcga taccgcgacc
    22741 ggcacgcccc acctgttagt agtaaccaac tatcgaacct ggcatctggt aatgaataac
    22801 aaaccccaaa tccggtttgt caaagcccat tcccagcgcc gttgtcgcaa ccagcacttt
    22861 aattttattt gccagcaacg cctgctccag atattccctg gcagtattag aatcttccgc
    22921 gccttcacaa gtcacgccgc tgtagtaagc aaatgcactg ataccatttt tccttaacca
    22981 ctcggcgaca agttcggcat cgcgagtagt cagggtataa acaattcccg atttggaaaa
    23041 ctgagggata accgttgcta accatgccag acgggatgag tgttcaccta agactaaggc
    23101 atcaagcgcc aaactttcgc gagccagcgt tccacgctga atcacaatgt cacccaattg
    23161 ctgacggata tcctcaacga cacggttatt cgctgtcgcg gttgtaccca gaacaggggt
    23221 attcgcaggt agttgcgcaa aatatctaat atacgtcggt aatccgggcg aaaatcgtgg
    23281 ccccaatcag agatgcagtg cgcctcgtcg accacaagca agccaatatg atcggctata
    23341 ggatataaaa ccgtttcgat aaagtcctgg tttgccaaac gttccgggga aaccagcaag
    23401 caatcgacct caccttgcag caacttatcg ctaatgcgct gccattcttc tctgtttgta
    23461 gaattcaacg tttcagccgt aatacctaac cgttcagcgg cggcaacctg gttacgcatc
    23521 agggcaagca aaggagagat gataattgtc gggccagcgc cgcgatcgcg gaagatttta
    23581 ctggcgatga agtacacagc acttttcccc cagccagtgc gttggacaac cagcagtttt
    23641 cgccgttggt tcaccaccgc gtctatggct tcccattgcc cttccctgaa agtagcggtg
    23701 gtatttccta acatccgctg taaaagcaat tcagctgcgt gcttttccat ctttatctcc
    23761 tgcgatcttt aggcagtgat tagaacggat tatttgcagg taaatttgcg agagagatca
    23821 aaaagatgaa gagaggagaa aagagaaatg aattactgcg agcaagctcg cagtaatttg
    23881 tcgcagtaat taactcaaca gctggaacgc aatcatccca acgatggcac cgacagtgcc
    23941 gaggatggtt tccatcatgg tccaggtttt cagcgtttcg gcttcggtcg cgccggtaaa
    24001 tttaccgaac aaccagaaac cagcgtcgtt aacgtggctg acaacaatcg aaccaccggc
    24061 gatacaaatc gacagcgccg ccatttgcgc accggagtag ttcagttgtt caataaccgg
    24121 catcaccagt cccaccgccg ttaaacaggc aacggttgca gaaccctgaa tgatgcgcac
    24181 tgcagcggcc agcacgaagc aggtgatggc aatcggcagg cccatgccgg ttaacgcttc
    24241 gcccagtgcc ggacctacgc cagagtcaac cagcacctgt ttgaacacgc cgcccgcacc
    24301 aatcaccagc agaatgatcc ccgccggttg cagcgcgtga ccgcaaatct ccatcacttt
    24361 gtcttttggc atgccctgac gcattgccag gccataaatc gctaccagac aagcaaccag
    24421 aatcgcggta aacggatgac caataaactc gaaccattcg taagcggtag agccttccgg
    24481 cacaaaacgc gcggcaatgg ttttcagccc taccagcacc agcggcagca ggatcagcga
    24541 caggctgaat ccgaaggatg gcattttgcc ttcgccgaga tgcggttcgc tgatgtcgtc
    24601 aggaatatgc agctcaacgt aacggctgat gaagttaccc cacagcggcc cggcaataat
    24661 cattcccgga attgccgcac acaggccaat caggatcatc cagccaaaat cggcgttcat
    24721 ctgcgatgcc agcagcattg gcgctggccc tggcaccagg aacgccgccg cggccgccac
    24781 gcctgcgaat aatgggatta ccagcttcac caggttcgta ccggtgtggc gcgccattga
    24841 gaaagcaacg ctaatcagca gaacaatcgc cacttcaaag aacagcggta gcgcacagac
    24901 cagccccgca aggccgatgg cataatgcgc gcggctgtga ccgaaggatt taagcatttt
    24961 gacggcaatc tgatcgactg cgccggtttc atgtaagatc ttgccaaaca tagctcccag
    25021 ggcgacaacc accgccagga agccgagggt gcctcccatc cctttttcca tcgtcgctgc
    25081 gattttatcg agcggcatac cggaaaaaag gccagccccc atggacacca ccattaaagc
    25141 caggaaagcg tgcatacgcg ccttcatgac taaaaacagc agcagtaaaa cagaccctac
    25201 tgctgttaaa acaagcgtta atgtagtcac tacttatttg ccttttttaa taacctcaat
    25261 ggtgcttgcc acaacacctt ccagcggttg atcgatatcc accaccagta catcggtttc
    25321 gtccgcaccc ggctcctgca gcgtttcaaa ctgcgtcacc aacatttggg ttttaaagaa
    25381 atggcctttg cgcgctttca ggcggctttc aatcacatca aaatcgcctt tcaggtagat
    25441 gaaagagaga ttcggattac cttcacgcag caagtcacga tagtgttttt tcaatgcaga
    25501 acagacgatc agcgacactt tattagtgcg ctgcatagca aacgcggcgt cgttcagcgc
    25561 ctgcaaccac ggtttgcgat cgtcgtcatt cagcggttcg ccagacgcca ttttttcgat
    25621 attgcgccgt ggatggagga aatcgccatc aagaaacgcg gcatgaagtt gatgcgccac
    25681 ttcacttgcg accgcagatt tgccgctgcc cgatacgccc atcaagacgt aaatgtggtg
    25741 atcatggtta gtcgtgctca aagtggtgcc cccacaatac aagaattaaa aatgttacgg
    25801 gtaactgtta tcggtaacat tgtccagccg gacaatatca gaagcaatat ccatccgtgc
    25861 cttaagtgta taagtgtgag ctacttcaaa tttgtgggct taaatagatc cgcccggtga
    25921 caaggtgaaa cctaaatcta acattttcgg tgtcacagat tcgccacgaa tacgcgccag
    25981 caggcgttca gcgccaatac tgcccatccg ctcacgcggc gtcagcacgc tcgcaagtcg
    26041 tggctccatc acctgaccaa tgtcatgacc gtggaaaccg gcaatcgcca tatcgtcagg
    26101 aacttttaac cccagacgct gacattcaaa cgccgcgccg accgccagat catcattagt
    26161 acagaacacg ccatccagct gcggatattc ccgccgtgcc tggcgaatca gttcaatacc
    26221 ggaagagtaa gaagaagatt gctcaaccat cacgctatat ggcaccaggc ctgcatccag
    26281 catcgcctgt tcgtatccct tctgtttgat gatagtacgt tcgtcgagac gtgcgccgag
    26341 ataggcaatg tggcgatgcc cgcgagcaat aatggctgtg gtcatctggc gtgccgcttc
    26401 aaagttatca aaaccgacgg cgatatcgag acacggcgac tggctgtcca tcaactccac
    26461 caccgggatc cccgccactt caatcatctt taaggtgcgc ggcgtgtggg tacgttcggt
    26521 gaggatcagt ccgtcgatat tccatgagag cattgattcg agacgttctt gctccatttc
    26581 cggtttataa ccgtagtggg ccagcatggt ctgataaccg tgcgcgtcgg tgacgctttc
    26641 gattccgcgt aatacttccg cgaaaacctg gttggtgaga gaaggtaaca ggacgccaat
    26701 cgcccggctg gtggcgttag agaggatatc gggcgcacga ttgggaatat agcccagttc
    26761 atcaagagcc gcggcaatct tgccgcgtag agcgacggaa acctgctccg ggttgcgtaa
    26821 aaaacggctg accgtcattt tggtcacgcc tacacggtca gccacatcct gaagtacggg
    26881 tcttttcttt ttcatcgtcc tgaaggtaca aaagagatag attctcttag tttaacacgg
    26941 acgcatcaga accttcccgg ggaaaacagt gcttgtttac aattattaga tatacactaa
    27001 atcaattgca tcgcattgtg ctaacgacgc tgcaaaaccc cggaagcgta catcagcacg
    27061 cgaccggagc gagcgaggaa gcctgcaaaa aggcaggctt caaagatgcc gttgagcttt
    27121 aaaccggcgg cagatcgaac agtaacactt cgctgtcgct atccgcatgg atggagattg
    27181 cctgctcatc ccagattgcc agaccatcgc tgatcgaggc tttcacgccg ttaatggtga
    27241 cattgccttt caccacctgg atccagacgc ggcgttcagc ggcaatctga tgcaccgact
    27301 gctcatcttt cagcaacgcc cagcggtaca gttccatatc ctgatgcact ttcaatgaac
    27361 catcacgcgc atctggcgaa agcaccagtt gtttgccctg cactgcatcg aagcgacgct
    27421 gttcataacg cggcgtaatg ccgttttctt ccggcatgat ccagatctga tacaggtgca
    27481 gacgctcggt gctgcttggg ttgtactctg agtgacgaat acccgtaccc gcactcataa
    27541 tctggaactc gcccgccgga acctgctctt tattgcccat gctgtcctgg tgctcaacag
    27601 taccttccag cacgtaggtc aaaatttcca tatctttatg cgggtgagtg ccgaagccct
    27661 gccctgcttc aatcacgtcg tcgttaatca cgcgcagcgc ggagaagccc ataaagttcg
    27721 gatcgtaata gttggcaaaa gagaaagtat gccaggagtc cagccagcca tgatttgcat
    27781 gaccacgttc atttgctttg cgtaagtaga tcatttgtat tcgccccctg aatgatttcg
    27841 atgggcttag tgtggacccg atctgcctgg gatgatagag ggtgaaaatt gacccctctg
    27901 ttcaaaaaaa ttgaactatt cgaggggcca tcaaacttac ttcgcgagag tcactgtgga
    27961 gggagaggct tgctcgaatc cacgttcaag tatttccaga ttcgtaagaa cttcagattc
    28021 cttgacgtaa tttggcgcac cgtgggtgat ggtttgatac aacgcatcat aaacgcgccc
    28081 gtaatcgccc atctccggct tcatctcttc tctgaccgtc acgccctcgt cattgacata
    28141 ctccagcaca ccgaccgaat catccgctgc gaatcccggt tcgcccggca taatattagc
    28201 cttcaggctg gtttcctgct ggtcgatacc atatttaata aacgaacctt tcttaccgtg
    28261 aacgataaat ttcggataat cgattttcac cagatggctg gttttgacga tggcttttag
    28321 atcgccataa aacagttgcg cttcgaaagt atcgtcagga ttggctttat tgcgcaggct
    28381 gcggatgtca taagcgacgt gatccgggcg accgaacaga gaaataatct ggtccatcgt
    28441 atgcacacca aggccataga acgcgccatc ctgcggcagc ccaggtttgg tttctgccac
    28501 cgggcggtaa tagtcaaaat ggctttccac ttcgacaatc tctcccagct tgccgctttc
    28561 aatcgctttt ttcgccgtca ggaagcagga atcaaagcga cgattctgat atggcgtgac
    28621 ggtcagccct ttgcttttcg ccagtgcaaa cagctctttc gcctgcgcaa gtgtcggagt
    28681 gaacggtttt tcgaccagca catttttccc ggcttccatc gcgcgcttcg cgtactcgaa
    28741 gtggctgtcc gcatgggtgc agacaacaac cagcttaaca tcgggatcgt ttagcacttc
    28801 gttgagatcg ctggtgaaat ggatatgaga gtaaatggga gcttgttctt ccggctttgc
    28861 atggcggcga aaaatatgcg cgacatgcca gctatccttg cggttaagta catacggcag
    28921 atggtaacgg gtggtgcttt tgccgaagcc aataaaggcg cagttgatga ccatgatttc
    28981 gtccttttta aggtggttat ttacacctta gcgcaaagcg gacgtggttc ctactggagt
    29041 gcgcataacg aacacaagca cttcccgagg ataaattgag aacgaaagat caaaaaaaaa
    29101 gccagcaccc ggctggctaa gtaatactgg aagcaatgtg agcaatgtcg tgctttcagg
    29161 ttctccgcga gggtcttcct gatcgcgaga caataataat cattctcatt cgcacttgtc
    29221 caacactttt tgcaaaaaaa tgcatttgac tcgcattcga aagtcaatga tgttgaaagg
    29281 gacatttacc ccaaagagga caaaggaatg agtgagatag tgatacgcca tgctgaaaca
    29341 cgggattacg aggccatcag gcagattcac gcccagccgg aggtgtatta caacacacta
    29401 caggtgcctc atccttccga tcatatgtgg caggagcgac tcgccgatcg tcccggcatc
    29461 aagcaactcg tcgcctgtat tgatggagac gtcgtgggcc atctcaccat tgacgtgcaa
    29521 cagcgcccac gccgcagtca tgttgccgat tttggtatct gtgtcgacgc ccgctggaag
    29581 aatcgcggcg tcgccagcgc cctgatgcga gagatgattg aaatgtgcga caactggttg
    29641 cgggtagatc gtattgagct aacggtgttc gtcgataacg cgccagcaat taaggtctat
    29701 aaaaaattcg gctttgagat tgaagggact ggcaagaagt acgcattacg taatggtgaa
    29761 tatgttgatg cgtattttat ggcgcgggtg aagtaaagaa tattctcaaa ttgcctgtta
    29821 ccaatgtgaa taattagttt tgttcatcta atttgagttt ttccttatat taccaccatc
    29881 attttgaaac aatttgtaac atcaagagac ctttgaatca ttactcgtta atatttctta
    29941 ttaaagagaa catattttcg caagcaatag ttattactgt ttgtttatgg atagagagtc
    30001 tacttaatgg gtacagcagc tataagtcat ttacgatatg atcttaacaa atatgccctt
    30061 tctttacgca aaaccgcaac acttgcttca acatttttta ttgagtcccc attagttaga
    30121 ttcgag
//
LOCUS       DBFIFI010000040        28989 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.040, whole genome shotgun sequence.
ACCESSION   DBFIFI010000040 DBFIFI010000000
VERSION     DBFIFI010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28989
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.040"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..1289
                     /gene="uraA"
                     /locus_tag="N7B39_001679"
     CDS             <1..1289
                     /gene="uraA"
                     /locus_tag="N7B39_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HGU2421681.1"
                     /translation="TRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPAT
                     VLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFCL
                     VSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTIII
                     SITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTLY
                     TPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVIS
                     GFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVMG
                     GVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGMA
                     LATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            1384..2085
                     /gene="hda"
                     /locus_tag="N7B39_001680"
     CDS             1384..2085
                     /gene="hda"
                     /locus_tag="N7B39_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HGU2421682.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(2223..2582)
                     /gene="arsC"
                     /locus_tag="N7B39_001681"
     CDS             complement(2223..2582)
                     /gene="arsC"
                     /locus_tag="N7B39_001681"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HGU2421683.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(2603..4066)
                     /gene="bepA"
                     /locus_tag="N7B39_001682"
     CDS             complement(2603..4066)
                     /gene="bepA"
                     /locus_tag="N7B39_001682"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HGU2421684.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRNAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            4279..5340
                     /gene="yfgO"
                     /locus_tag="N7B39_001683"
     CDS             4279..5340
                     /gene="yfgO"
                     /locus_tag="N7B39_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HGU2421685.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(5378..6226)
                     /gene="focB"
                     /locus_tag="N7B39_001684"
     CDS             complement(5378..6226)
                     /gene="focB"
                     /locus_tag="N7B39_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
                     /protein_id="HGU2421686.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHFAPTSFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSICYRAI
                     YLRQES"
     gene            complement(6248..8260)
                     /gene="hyfR"
                     /locus_tag="N7B39_001685"
     CDS             complement(6248..8260)
                     /gene="hyfR"
                     /locus_tag="N7B39_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HGU2421687.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLHLLASNGCESSLLIPLTFGN
                     HTPGALLLAHTSTTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRAIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(8290..8703)
                     /gene="hyfJ"
                     /locus_tag="N7B39_001686"
     CDS             complement(8290..8703)
                     /gene="hyfJ"
                     /locus_tag="N7B39_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HGU2421688.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVTFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            complement(8696..9454)
                     /gene="hyfI"
                     /locus_tag="N7B39_001687"
     CDS             complement(8696..9454)
                     /gene="hyfI"
                     /locus_tag="N7B39_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HGU2421689.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKRHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGQEICDRLLRHLSND
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(9451..9996)
                     /gene="hyfH"
                     /locus_tag="N7B39_001688"
     CDS             complement(9451..9996)
                     /gene="hyfH"
                     /locus_tag="N7B39_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HGU2421690.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPTNALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTN
                     KADLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLRAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            complement(10006..11721)
                     /gene="hyfG"
                     /locus_tag="N7B39_001689"
     CDS             complement(10006..11721)
                     /gene="hyfG"
                     /locus_tag="N7B39_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HGU2421691.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVQYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDSETYPFINEGNSGARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(11711..13291)
                     /gene="hyfF"
                     /locus_tag="N7B39_001690"
     CDS             complement(11711..13291)
                     /gene="hyfF"
                     /locus_tag="N7B39_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HGU2421692.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGHSATRTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     EHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLEAAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(13296..13946)
                     /gene="hyfE"
                     /locus_tag="N7B39_001691"
     CDS             complement(13296..13946)
                     /gene="hyfE"
                     /locus_tag="N7B39_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HGU2421693.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLSMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(13958..15397)
                     /gene="hyfD"
                     /locus_tag="N7B39_001692"
     CDS             complement(13958..15397)
                     /gene="hyfD"
                     /locus_tag="N7B39_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HGU2421694.1"
                     /translation="MENLALTTLLLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVCTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWVLISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFALSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMALVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            complement(15414..16361)
                     /gene="hyfC"
                     /locus_tag="N7B39_001693"
     CDS             complement(15414..16361)
                     /gene="hyfC"
                     /locus_tag="N7B39_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HGU2421695.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPISSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGCAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(16372..18390)
                     /gene="hyfB"
                     /locus_tag="N7B39_001694"
     CDS             complement(16372..18390)
                     /gene="hyfB"
                     /locus_tag="N7B39_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HGU2421696.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHEGVTLVARFATPFDFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNIFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISALPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTQVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGLAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(18390..19007)
                     /gene="hyfA"
                     /locus_tag="N7B39_001695"
     CDS             complement(18390..19007)
                     /gene="hyfA"
                     /locus_tag="N7B39_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HGU2421697.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCVQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENAPTQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGREDPLSLTQEQR"
     gene            complement(19260..19730)
                     /gene="bcp"
                     /locus_tag="N7B39_001696"
     CDS             complement(19260..19730)
                     /gene="bcp"
                     /locus_tag="N7B39_001696"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HGU2421698.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(19730..20302)
                     /gene="gcvR"
                     /locus_tag="N7B39_001697"
     CDS             complement(19730..20302)
                     /gene="gcvR"
                     /locus_tag="N7B39_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HGU2421699.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            20448..21326
                     /gene="dapA"
                     /locus_tag="N7B39_001698"
     CDS             20448..21326
                     /gene="dapA"
                     /locus_tag="N7B39_001698"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HGU2421700.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            21343..22377
                     /gene="bamC"
                     /locus_tag="N7B39_001699"
     CDS             21343..22377
                     /gene="bamC"
                     /locus_tag="N7B39_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HGU2421701.1"
                     /translation="MAYSVHKSRLATVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            22590..23303
                     /gene="purC"
                     /locus_tag="N7B39_001700"
     CDS             22590..23303
                     /gene="purC"
                     /locus_tag="N7B39_001700"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HGU2421702.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            23471..24334
                     /gene="ypfJ"
                     /locus_tag="N7B39_001701"
     CDS             23471..24334
                     /gene="ypfJ"
                     /locus_tag="N7B39_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HGU2421703.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            24349..26364
                     /gene="tmcA"
                     /locus_tag="N7B39_001702"
     CDS             24349..26364
                     /gene="tmcA"
                     /locus_tag="N7B39_001702"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HGU2421704.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHFKRVLTADNDAIFWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQILQQLLTMPSGVAAVTAARGRGKSALAGQLISRIAG
                     SAIITAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGVGQQLIASALQYRPGLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNNAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNVSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNDVRTERLRDRITQWQFFH"
     gene            26438..27136
                     /gene="ypfH"
                     /locus_tag="N7B39_001703"
     CDS             26438..27136
                     /gene="ypfH"
                     /locus_tag="N7B39_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HGU2421705.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(27217..27417)
                     /gene="ypfN"
                     /locus_tag="N7B39_001704"
     CDS             complement(27217..27417)
                     /gene="ypfN"
                     /locus_tag="N7B39_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HGU2421706.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(27445..28572)
                     /gene="dapE"
                     /locus_tag="N7B39_001705"
     CDS             complement(27445..28572)
                     /gene="dapE"
                     /locus_tag="N7B39_001705"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HGU2421707.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKAVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(28576..28932)
                     /gene="yffB"
                     /locus_tag="N7B39_001706"
     CDS             complement(28576..28932)
                     /gene="yffB"
                     /locus_tag="N7B39_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HGU2421708.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNIITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQKFFHEV"
BASE COUNT         6989 a         7619 c         7800 g         6581 t
ORIGIN      
        1 tgacgcgccg tgctatcggg gtgagtgaaa gaccgccact tttacagaca atcccgctta
       61 gtttgcaaca tttgttcgcc atgtttggtg caaccgtcct ggtgcccgtc ttatttcata
      121 ttaacccggc gactgtactg ttatttaacg gtattggaac gctgctgtat ctcttcatct
      181 gtaaagggaa aattccggct tatcttggtt ccagctttgc ctttatttca ccggtattgt
      241 tactgttgcc gttagggtat gaagtcgcgc tgggcggctt tattatgtgc ggcgtgctgt
      301 tctgcctggt ttcttttatc gtgaagaaag cggggaccgg ctggctggac gtgctgtttc
      361 cacctgcggc aatgggcgca atcgttgccg tcatcggtct ggagctggcg ggcgtagctg
      421 ccggtatggc gggtttactc ccggctgaag ggcaaacgcc agactccaaa accatcatca
      481 tctctattac caccctggcg gtcacggttt taggttccgt gctgttccgt ggtttcctgg
      541 caattatccc gattttaatt ggcgtgctgg tggggtacgc gctctctttc gcaatgggaa
      601 ttgtcgatac cacgccgatt attaatgctc actggtttgc gctgccaacc ctctatacgc
      661 cgcgcttcga gtggtttgcc attctgacta ttctgccagc ggcgttagtg gttattgccg
      721 aacacgtagg gcacctggta gtaacggcta atatcgtcaa aaaagatctg ctgcgcgatc
      781 caggtctgca ccgttcgatg tttgctaacg gcttgtcgac cgtgatctcc ggcttctttg
      841 gctccacgcc aaacaccacc tacggggaaa acattggcgt gatggcgatc acccgtgttt
      901 acagtacctg ggttatcggc ggggcggcga ttttcgctat cctgctttcc tgcgtcggta
      961 aactggctgc cgctatccag atgatcccat tgccggtgat gggcggcgtt tcgctgctgc
     1021 tttatggtgt catcggtgct tccggtattc gtgttttgat cgaatcgaaa gtggattaca
     1081 acaaagcaca gaacctgatc ctgacttccg tgattttgat catcggcgtc agtggcgcga
     1141 aggtaaacat tggcgcggcg gagctgaaag gtatggcgct ggcgaccatc gtcggtatcg
     1201 gcttaagttt gattttcaaa ctgattagtg tgctgcgccc tgaagaagtg gtgctggacg
     1261 cagaagacgc ggatataaca gacaaataat gtaccgaccg ggcagtgttg ctgcccggtt
     1321 caaacatcat gggattctgt ggtaaacttc tcgcgatttt gtgaaatcct ggttgaggta
     1381 tctctgaaca caccggcaca gctctctttg ccactttatc ttcctgacga cgaaaccttt
     1441 gcaagtttct ggccggggga taactcctct ttactggccg cgctgcaaaa cgtgctgcgt
     1501 caggaacata gcggttacat ctatctctgg gcacgcgaag gcgcggggcg cagccatctg
     1561 ctgcacgcgg cttgcgcgga attgtcgcag cgtggcgatg cggtgggcta tgtcccgctg
     1621 gataaacgca cctggtttgt tccggaagtg ctcgacggta tggagcattt gtcgctggtc
     1681 tgtatcgaca acattgagtg tattgcaggc gatgagttgt gggagatggc gattttcgat
     1741 ctctacaatc gaattctgga atcgggcaaa acacggttgt tgatcaccgg cgatcgtcca
     1801 ccgcggcagt tgaatctggg attaccggat ctcgcgtcgc gactcgactg ggggcagatc
     1861 tacaaattgc agccactttc tgatgaagat aagttgcagg cgctacagtt acgcgcgcgt
     1921 ttgcgtggtt ttgaactgcc ggaagatgtg gggcgtttct tgctgaagcg gctcgacaga
     1981 gaaatgcgca cgctatttat gacgttggat cagttggatc gtgcgtcgat taccgcgcaa
     2041 cgtaagctga ccattccgtt tgtgaaagaa attctgaagt tgtagataag gtgtttattg
     2101 tcggatgcgg cgcgaacgcc ttatccgaac tacgcgaagt taatccatag aattcgtagg
     2161 ccggataaga cgtggcaagc gtcgcatccg gcaatgtgct tcaactagcg atggagcaca
     2221 tcttaaccca cgatttccag tacctgctcc ggtggacgac caatccgcgc tttgccattc
     2281 gcgaccacaa tcgggcgctc catcagcttc ggattatcca ccattgcctg aatcagcgcc
     2341 tcttcgctaa gcgaactgtc agccaggttc agttctttat aaagatcctc tttctggcgc
     2401 atcaattcac gggcgctatt catccccagc attttcagca aatcgcgcag cgttgccgca
     2461 tcggcgggtg tctcaaggta gagcaccact tctggctcca cgccgttttc tttcagcagg
     2521 ttcagcgttt cccggctctt tgaacagcgt gggttatggt agatttttac ctgtttggtc
     2581 attgttcttc ctttaatgcg aattacatct tggtataagg cttaaagcgt tcctgcagct
     2641 ggcgcaactg gtcgatgcgc gcatcgtaac gcgcttgttg caggctgcct aatttcacct
     2701 gcgaactggc gctactcaac agcgaaatgg cctgatcgag tcgtccggcg agcgcataac
     2761 cttctgctcg cgcagccagc tcctgatcgc ggttatttag cgcggcttcc gcctgtgcca
     2821 gcaaatccca gccgttgctg tcatctttat tattaaaggt gtagcgatta agaatattgg
     2881 ccgcttcttg tggttgaccg ccttgtagat aagcgttcgc caggttgagc tgcaacacag
     2941 gattggtgcg caaatcgcgg gcatttttca gacgattgat cgcctcattg gctttgtttt
     3001 gcccaagatc gatatcagta gccagatcga gataccatgc gttgccaggt tctgccgcca
     3061 gtaacggttg cagcgttttt cgcgcctcgt cgtatttatt ggcttccatc gcctgtaaag
     3121 cacgaccata ttgcgccgct cgttgctgac gaacgtttcc tttcgcccat tcatccagca
     3181 aatcactggt gagctggtta cgtccggaat tatacatccc cagtgtgcgc gctttcgcca
     3241 gatagaaatc ttccgacgac tgcaccacca tcgggcgcat ctgattagca cggttgcggg
     3301 catctgccag acgactttcc ggcaacgggt gagtcagtaa aatttccggc gggcgcgagg
     3361 agtaacgcgc ctgatcgagt aatttttcga ggaaggttgg catcgcctgc ggatcgaatc
     3421 ccgagcgttg cagcacctga ataccaatgc ggtccgcttc ctgttcattt tgctgggtga
     3481 aactgatcat cccctgacgc gttcccgcca gtgtaccggt cagcgccgcc atccccgcct
     3541 gcggactggc catcgccagt aaaatagaac ctaacgcgcc gacccaggtc agcggcgcgt
     3601 tgcgctgctg atcttccatc gctcgcgcca ggtgacgttg ggtgacgtgg gagatttcgt
     3661 gcgccataac tgaagccagt tgactttcgt tatcggaata acggaacagg gcagagtgca
     3721 gcaccacgtt gccgccaaag aaagcaaagg cgttaatttc gtcgttgttg atcagaaaga
     3781 aatgaaacgg tgtcttaacc gaattggcat gcgaaaccag acgcatcccc agcgaattaa
     3841 tatattgcgt taacagcggg tcattaatta acggcgcgct gccgcgtagc tggcggacat
     3901 aatagtcgcc catctgcatt tcctgaccaa tggaaagcgt gcttcctgcg gaggttccca
     3961 tatccggcaa ggtgtctgcg ctgtcggcaa atgccggggc tacctgacca atggtcatag
     4021 cagcaatgag ggttgcaacc aggttttttt tcaactgcct gaacataacc tctatcctgt
     4081 atttctgaag atagtcattt gaccgatact gtgaaatatc gttcccaccg cgccgccttt
     4141 gagtgtaacg gcgaagatta gcccactcaa atgacttttc agtttcctga atccgtcata
     4201 aaaagcgaag tcttttttgt gacatttcga tacaattgcg gatcgcaatc ccgctcttga
     4261 ggttcaggga aggtctttat gctcgaaatg ttgatgcaat ggtatcgccg ccgttttagc
     4321 gacccggaag cgattgcctt gctggttatt ttagttgccg gatttggcat tatctttttc
     4381 tttagtggcc tgcttgctcc gttgctggtg gctattgtgc tggcctattt gctggaatgg
     4441 ccaaccgtgc gcctgcaatc tattggctgc tcccgccgct gggcgacgtc gattgtattg
     4501 gtggttttcg tcggtatatt gctactgatg gcgttcgtgg tactgcctat cgcctggcag
     4561 cagggcatct acttaatccg cgatatgccg gggatgctca ataagctttc tgactttgcc
     4621 gccacgttgc cgcgccgcta tccggcgtta atggatgcgg gcattattga tgcaatggcc
     4681 gaaaatatgc gcagtcggat gctgaccatg ggcgattcgg tggtgaaaat ttccctcgcc
     4741 tcgctggtcg gtttgctgac cattgccgtc tatctggtgc tggtaccatt gatggtcttc
     4801 ttcctgctga aagacaaaga gcagatgctg aatgccgttc gccgggtgct gccgcgtaac
     4861 cgtggactgg caggacaggt gtggaaggag atgaatcaac aaatcaccaa ctatatccgc
     4921 ggcaaagtgc tggagatgat cgtagtaggg atcgccacct ggctgggatt cttactcttc
     4981 gggctgaact attcgctgct gctggcggtg ctggtcggct tctcggtact tattccgtac
     5041 attggcgcat ttgtggtgac cattccggtg gttggcgtgg cgctattcca gtttggtgca
     5101 ggcactgaat tctggagctg tttcgcggtg tatctgatca ttcaggcgct tgacggcaac
     5161 ctgttagtac cggtgttgtt ctccgaagcg gttaacctgc atccgctggt gattatttta
     5221 tcggtggtga tcttcggtgg tttgtgggga ttctggggcg tattcttcgc cattccattg
     5281 gcgacgctga tcaaagccgt gatccacgcc tggcccgatg ggcaaatagc gcaagaataa
     5341 tcttcagccc gacgaactgt ttcgccgggc tgatttttca ggattcctga cgtaaataaa
     5401 tagcccgata acaaatactc accagcactg caccgccgat aatattaccc agcatcaccg
     5461 ggagcagatt ggcggtaata aaatggctga ccgtcagtgc aggaaaattg tctgcgctac
     5521 tgtgcgccag ttgccagaag gaggtagggg cgaaatggcg aatggcaatg gcgaacggaa
     5581 tcacaaacaa attggcgata cagtgctcaa agccactggc gacaaacagg gtgatgggca
     5641 aaatcatggc gacgattttg tcgcataacg aacgcccgca ataactcatc cacagcgcca
     5701 ggcaaaccat cagattgcac ataatgccga ggctgacaga ttcagtaaat gtatgatgca
     5761 ttttgccctc ggcgcagtgt aaaaccgcga ctccccacat cgcgttttca ctcatcacca
     5821 gcccggaaaa ccagattaac aaactgaaac aggcaatacc tgccagatta ccgcaggcca
     5881 ccagaagtgc gttaatcagc caagttcgcc aactaataac gccccgactt tttgccatca
     5941 ccgtcattac cgacgaggtg aacaggctgg tgccgcaaac cgccagcaaa ataaagccga
     6001 gtgtaaagca aaggccgccc accagatggg ttaatgcctg tgaagacggg gcatcggcga
     6061 taacggaaag gtaaaaagta aatccgatcg ccataaacac gccagcggac atcgccatta
     6121 aaaagaccga cactttactg cgggcaattt tatgggcggc aatccgttca gcgattgccg
     6181 cttttctggc gctcaactgc aagtcgaaag agagtttgtt tctcatgcat actcctgcaa
     6241 aagcagatta caacacctcg cgaaccgaga tccccagacg ctgcattcgt gacagcagcg
     6301 tggtgcgctt catccctaat cgtgtcgccg cgccacgggg cccggcaaca atgccattgg
     6361 tttctcgcaa tacctgaaca atgcgctgac gttcttcttc gtcattctct ggcgtcgtcg
     6421 ggtgcagcaa ctgcgccatt gaactgcgaa gcgctgaatc ttcatttagc gtcggcagta
     6481 aacggctttg tcggacatta agatgtaaat tcagactgtt accgcgagtc agcagtaccg
     6541 ctcgctcaat cacgttttcc agctcgcgca cgttgcccgg ccaatcccac gacatcaact
     6601 ggcgtagtgc ctcggtcggg atggcgtcaa ttgcgcgatt catatggcgc gccatttttt
     6661 gcgtgaaatg ttttgctaaa agagggatat cttccggacg gtctcgcagc ggcggcaatt
     6721 ccagtgggaa gacattcagg cgataaaaga gatcgctgcg aaactggcga tcttcaacca
     6781 tttgccacaa atcacggttg gtggcggcaa tgactctgac attcaccggg atcgttctac
     6841 tcccgccgag acgctcaatt tcccgctcct gcaatacgcg cagcaattta ggctgaagtt
     6901 ctaacggcag atcgccaatt tcatcgagaa acaacgtacc gccatcggca atttcaaaac
     6961 gaccacgatg ggtattaatc gcaccagtaa aggccccttt gtcatgaccg aataactcac
     7021 tttccagaag actggcgggg atggcagcgc agttgatttt gaccagcggc ttgtcgcgtc
     7081 gcgggctaag ttgatggatc gctctggcga tcacctcttt gccggttccg gtttcaccac
     7141 aaatcaacac cgtactgtcg ctcttcgcca caatatctac ctgctggagc aggtcttcca
     7201 ttgcctggct ttgatagata atgtcaccga cgcccagatt cgaaaggagc tgctcgctaa
     7261 gctggtggtt ttcttgctgc aaactttcct gcaaatcggt catgctacgc caggcatcgg
     7321 cattgccaac ggcaatagcg atgcgatcgg ctatgtgttg tagtagctgg cagttttcct
     7381 cactaaagag agtggtagag gtatgcgcca gcaacaatgc acccggtgta tggttgccaa
     7441 aggtaagcgg tatcaggagc gaagattcgc agccgttaga tgcaagtaag tgtaataacg
     7501 ggtcgcgttg ccagagaaac agatcgtctg cacggtgcgt caaggtcggt tgtcgggttt
     7561 gtagcgtttg tgtcagcaat acactgtcgc caggcatatt gcgtggcaga cacgcacaat
     7621 gtgaggcaga aagatcgctg caccacaggc tgaacttctc gttctttcga tgatcgccca
     7681 gtaccatact gactgaagcc agaccgaaaa aatgatggat ctcacgagcg acgtcagcga
     7741 tcagatcgtc gagatcaaga tgtgacagca ctgcattagt gatatccacc agtacccgat
     7801 gatgatcgcg atctttctcc agcaaacgca gcgccacctg ttgttcttca tcattgagcc
     7861 atgattgcag aaccggggtg ataatgcgtg ccagccagtt aaacagtgtg caatcctggg
     7921 gagtaaaacg actgccgtgg gtgcgaataa agcgtagctc cgccattcca ctactgccga
     7981 gaaggacggc gtgacgcgaa taatcgacca tctcgccctg atactctgtt gttgccgtca
     8041 catcgctgcc atcatcatgg tagcggtaat agaagttgct tttgagaaca tgcagttcaa
     8101 taagttgtac cgggctgaac ggcggcggca gcgggatgaa ggcgcgtaat aaagacgcct
     8161 tgccgtgttt ctgcgctaac cgctctgcga gcattccgtt taccgcttca attgttattc
     8221 cttgcggcgg ggcaaacatc gcctcgtctg acatagccat caacagtcct tattaatgag
     8281 aaatccttct cacgcaattt ttctggccat cagatagata gccggttcca gcacaatttc
     8341 atccaacaga tgaatgaatt gaatgctacg cgcttgccac acagacgtcg cgtcatctgc
     8401 cagtggcgcg aatgcccggg tcaacagggc ggtgtggctg gcgtcaataa caatctcacc
     8461 aaaagtcatc acccccagca tcttacgtcg ggcttgctcc tcttcgacca gtgcaagcca
     8521 atcttcgtat tccgtcagtg ggcagcggaa ggtgacattc agacaatcaa tcacgccaac
     8581 gtgatgaccg atagccagcg agtaatacat tacctgggag ctgtgttccg gcatctcgtc
     8641 gctactggcg acaaactttt ttcgcagcgt ccagaaaaca atttccccgc actcttcagt
     8701 catgtaaccc ccggagtacg cgaaagagtt gctgaacgat actattgaga cgtggatcgt
     8761 cggcatcgcg caaccaggtg ttaacccgat ttcctgtagg atcgttgctt aaatggcgca
     8821 ggagccgatc gcaaatttct tgcccctgac gatagcccgc cagccgccgc gcttctcgct
     8881 caatggcgat acgctgtgat ggcgggatct gcggccacaa cggttgcata gtcaccccgg
     8941 tgggatcgcg ataatccacc gcgtgaatct tctgttgcag caaaccgagc gccacggcga
     9001 aaccgtgaat ggtggcggcc ggtgttggcg ggcagccggg gatccaaaca tcaatgggga
     9061 caatggtgtc gctgccgccc cagacgctgt agagatcgtg gaaaataccg ccgccgacac
     9121 cgcacgcgcc gtaggaaaca cagattttat gatcgggggc agactcatac gcccgaagtg
     9181 caggcatacg catcgcccgg gtgactgcgc cagtaaataa caaaatatcg gcgtgacgcg
     9241 gtgatgaaac aaccttaatg ccaaaacgtt ctgcgtcgaa tacgggtgtg atggcggcaa
     9301 aaatttcgat ttcacaggcg ttgcagccgc cgcaatcgac gcgataaacg taggctgagc
     9361 gacggatatc ctgtagcaaa tgccgcttca tcttttgcgt ttgctcgtcc agcgtgatgg
     9421 gctggctgac atgttgtgta agcactggac tcatagctgc tccttagcca ccagcggtac
     9481 atctgtatcg tcgttgatca gcgtcgcgcg ttgtttgcac tccgggcaga cgctcgcttg
     9541 cgcccgcaac atttcgcggt tttgtggcgc attttgttgc tgtgccaaca attcagcagc
     9601 cagtgcgacg gttttttgcg gggcaaacgg gcgttcgcaa cggctgcaac gttgtagatg
     9661 gaacgtcgcg cgggtataga gatcggcttt attggtgacg gtcagttcaa agttattggt
     9721 aagctggatg gctctggtcg ggcacacttc ttcacaacgt ccgcagtaaa tacaacgccc
     9781 cagatagagc tgccaggtgc gcgaattttg ctggtcgtcg gtctggatag tcagcgcatt
     9841 tgtcggacaa gcacaggcgc aggcaccaca ggcaatacat tggctgggca tcaggtccgg
     9901 tttcccgcga aagcctgggc tgacctccag tggcgcgaag ggatatttca ccgtcgcggt
     9961 tccggcgcgc ataatagttt tcagtaactt cagcatcttc tgtccttact tcagcggcga
    10021 acggttacga tcaatgccgt agcgttcgat ctctttatac ggcacggttt ttgactggcg
    10081 cttgcgcaca tcaaccagcg tcacacggtc ggtacaggag tagcagggat caaggctacc
    10141 gataatcagc ggtgcgtcag aaacggtatt gccgcgcagc atgtaacgca acaccggcca
    10201 gttggcgtag gtggcggcac ggcagcgcca gcggaacaat ttttggttat caccgagcat
    10261 gctccagtgc acgtcttcac cgcgtggcgc ttcaacaaag ccgagcgcga atgcgtgagg
    10321 tttatagcta aagccttcgg tcagcagtgg ggtatccggc atgttgtcga gagcaaattc
    10381 cagcattgcc agcgaatcaa acgtctcttt gacacggacc atcacgcggg agaaaacatc
    10441 gccgccggtg aaggtaaaca gtgtttttgg aatattgccg tagtcggcgt aggggtgatc
    10501 aaagcgcaaa tcacgggcaa aaccactgcc gcggatcagc ggccctacag ggctataatc
    10561 acgggcgatt tgtcggtcga gaatgccaat gccctgagtg cgttgttcca tattcggcgt
    10621 ggcaagcagc atttctacca gctccgacac gtcggcgcgc atctcgcgca ccagtttcag
    10681 cgtttgcaga cgttgctctt tgagaatatc gcggcgaaca ccaccaatca gattcagacc
    10741 gtaggtttta cgcgacccgg tcagcaattc cgccattgtc atcgactttt cccgcacgcg
    10801 gaaaaattgc ataaagccgg tatcaaatcc aacaaaatgg caggagaggc caaggttgag
    10861 caaatgactg tgtagccgtt cgacttccag cagaatcgag cgaatggtat gcgctcgttg
    10921 cggcacctca atccccagtg cattttcaac cgagttggta taggccacac tgtgggcaaa
    10981 accgcaaatc ccacacacgc ggtccgataa gaaggtcact tcgttgtagc ccatccgcgt
    11041 ttctgccagt ttctccatgc cgcgatggac ataaaacaga cggtaatcag catcgacaat
    11101 ttgctcgcca tccacaaaca agcggaagtg acccggttca tcggaggtga tatgcagcgg
    11161 gccgacaggg atcacccgcg caccgctgtt gccctcattg ataaacggat acgtttcgga
    11221 atcagtcgtc ggttcagggc gcagtcgata atccatcgca tctttgcgca gcggatgcat
    11281 atcttccggc cagtcatcgg gcaacaccag gcgacgctga tccggcaggc caaccggaat
    11341 cagcccgtac atatcacgaa tttctcgctc gccccagacc gcggcaggga cgcgcggtgt
    11401 gactgacgga aactcccgac tgtcggcatc gaccagcgcc ttcaccacaa tccagcattt
    11461 ttcggcccct tccattgaaa gggcataata aaccgcgtaa tgaccgttaa gtgtccgctc
    11521 gtcgttgcca aacaggaccg gaagccagcc atcatgttga taataaagat actgtacaac
    11581 gtcaggcagc agattgattt tcaccgtaat ggtgacctgt tcaggcgttt gtcgctcttc
    11641 atccagcacc gcgccgggga actgcgtttt cagggcggcg agaatcgctt cgccacgatt
    11701 tgacgatgaa ttaacgttca ctgtgtttct ccgaaacaga tgcggtgcct gaaggcaaaa
    11761 aatcatgcca ggtgctacgt tgtgcaggca ggtcgtgcgt ccctgagagg actatagtgg
    11821 aagcgcccgc caggatcctg atgacaggtt gtggaatatg cgttcccatc gccagcatca
    11881 tgaccagcag aatcaccatt ggcgaggtgg tcaaccagcc gagatcaccc cggttaacgg
    11941 cctgcggcgg tttcgccatt aacacccgcg cagccatccg taccaggccc gccagcacca
    12001 gcgttaacag caataacagc aggacgataa tcagcaggtg attacgtgcc agtccggcgg
    12061 taacggtcat aaattcgcta agaaaaatgt tgaagggcgg catccctgcc agcgccagcg
    12121 caccgccgcc aaacagcacg gcggtaaatg gcatgatttt gagcatccca cagacgacgt
    12181 tgagatcgcg cgtgccgtac ttgagcagta cattgccgga accgcagaac agcagcgttt
    12241 ttgccagact gtggtttaag gtgtgcagca gcgcggcaaa aattcccagc ggcccgccaa
    12301 tgcctagcgc caccgcgacc agtcccatgt tctccacgct ggagtacgcc agcagacgct
    12361 taatgtcccg ctgtaccaga atgaaaaatg ccgccacggc aaccgacaac atgccgaaga
    12421 tgagcaacaa ccggttgggg aaatcgctgc cgatggcctg gcaaataatg atgtaatagc
    12481 gaatcagcac caacagcgcg cagttcagca atacggcgga gagtagggcg ctgaccggac
    12541 tcggcgcttc actgtgagca tccggcagcc aggcgtgcat gggaaatagc ccggttttgg
    12601 taccaaagcc aattagcaca aacacaaagg ccaacagcat taatgttgga tcaagcaagg
    12661 acgattgctt aagaacctcg ctccagaata tcgccatttc tgcctgcggc ataacgctgg
    12721 cggcgttggc gtatactagc acggtaccga acagaccaaa agcgacacca acagtacaaa
    12781 taatgatgta cttccatgct gcttccagcg atgaacgctg accgtaaatg cctaccagaa
    12841 acgccgagct taaggtggtg gcttcgatcg ccgcccacat cacaatcagg ttattgctgg
    12901 taacaaccag cagcatggtg aacaaaaaca gatggaagaa accgtagtaa tcgcacagcg
    12961 taacgggtga aagctcgccg tgctcaactt catgacgcat gtagccaatc gagtaaacac
    13021 cggtgagaaa gccaatcaca ccaagaatgg cgaggaacaa accgcccaga ccatcaatat
    13081 gaagccacag tcccgcagcg aatatttctc ctgcatcagc ggcagtttgg accacccaga
    13141 gtgccagaat cagcagcagt gtgatcccta agctatgtaa tactgtcacc gtgcgagtcg
    13201 cagagtgccc tcgtttccgg caggcaaaac agagcagcga aaaaagcagc ggagtgagca
    13261 ggagtaaagc gaacatcaca gaataactca tctcattatc ccttcagcgc ggtcaagttg
    13321 ttcacgtcca gcgtgccgtg ggtacgccat atttttcttg ccagtaacac catcacaatg
    13381 acggcgaaga tggcgtcggt agcgatacca atttccacca gttccggtgc acgccaggct
    13441 agaagcgcca gcaccagatg ggagccgttt tccatcaggc agtaaccaaa aatttgccgc
    13501 aggatattgc gctggctaac aatgcacagc aggccaagca gaaaatgacc taacgccacc
    13561 gccagcgctg gtttcagccc ggtagccatc gatagcttca cgggctgaac gacaaatgcg
    13621 caaagcagga caattaacgc cgcgagcagt gccatcattg ccggaccgaa taacgctttt
    13681 tccgggatgt tctggggaat atttcgtgca gcgtaagtca tgattaacgg taccagcagc
    13741 actttggtga taaaggcgct ggcggaccag atcagcagtt gctctgcggc gaacaggcac
    13801 gagagggtgg cgaaaataga caccagcacc agcgactggc aggcgtaaaa tccgcatgac
    13861 aggcgatagc ttttgacgct aatcacaaac agcgatgtca gcatcatcag tcccgccaga
    13921 ttatttacga tcatagaacc ggtcattcat catctcctta ctgcaaccag gtcgcggcga
    13981 ttacgctgga aatcagcgac atcacaatca gtacaatcag caccaggcgc attgaccctg
    14041 gcagcggtgc ggcatcggcg acggcctcgc tcggtttgcc ggggacgacg cgaccaaacc
    14101 agcgaataaa ccaggcgaaa ctggcgaccg attcaatcat cagcagaatc atggcgggca
    14161 gcaggatcca gtactccact gacaacgcaa aaccggcagc aaacagcggg aatttactga
    14221 agaagccgtt gaacggcggt acgccggtaa tcgccagcgc tgccacgcag aaacccacgc
    14281 ctggcaacgg cagggtgtgc aatacgccac gcagacgcgg caacaagcgc gtgccgcagc
    14341 tgtaactcag cgcacctgct acaaggaaaa acaggctttt agcgaacgcg tggttgacga
    14401 tgtaggcgat gctgccctcc agcgccagcc gcgagccgaa gatggagagc gacaagccga
    14461 agaacatcca gccaagttga gtgatggtcg accaggccag caaccgcttc atatcctgct
    14521 gtggcaaata catcagaaat ccataaagaa tggtgaccag ggccatgacc atgccaacgc
    14581 cgccaatcac atgcgggata ttgccgccgt cgataatggc gcgggcaaaa atgtaaacgc
    14641 ccactttcac catcgatgcg gcgtggagat aggcgctgat cggtgttggc gcttccattg
    14701 cgtccggtag ccacgcttgc atcggtagct gggccgattt cccccacgcg gcaaacagga
    14761 tgccgccata aaccagataa cgtgcgtcgc cgtgtaactc gctcatcgcg ctaagcgcaa
    14821 acgttccggt ctgcaaaaac agcgtggcgg cggcaagata caaccccaac gaaccgatat
    14881 gggtgataag caacgctttt agtgctgaac gctgcgcttt atcgctctga taataactga
    14941 tcaacaccca ggagcagccg cccgtaattt caaaaaacaa caactgaccg agcagcgtcg
    15001 aggagagtac cagtcccgcc atcgcgccga taaacaccag caaaaatgcg taataacgat
    15061 tcgtgccgtt atgcgggtgt tcgcgatttt tatccgtcag ataacccgtg gagtagatcg
    15121 tgaccagcaa accgagaaac accaccacaa acagaatcag cgtacacacg cgatcaatga
    15181 ccaggccaaa caatgccaca tcccccacgt tgaccaacgt caacgtgacg gcaactttat
    15241 cggcctgata aaacgcggag atcagcgaca acatgcacag cgtggtcagc gcggcgaaca
    15301 aaacccccca ttcggcggcc cgacgttgtg gcgaaaacga aacgaccagt gcgccgataa
    15361 aaggcagcaa taacgtcgtc agagcaagat tttccatatt ccgtcagtgc tccttacaga
    15421 ccggttaacc agaagaccca tgcaagcgca gcaaggctga agccaagcca ggtcacatgg
    15481 tgaatgagta aaaaacgccc gcgtgccagc gtgttttctg cgattgaggc cagtacaaaa
    15541 atcagcaaaa ccttgagcag cgtaacgaca agtgaagtca gcaggcaggc gagagaaagt
    15601 tcttgcgcgc acccaaaggg cagaaacagg gccacaaaca gtgctgccat cacgacctgt
    15661 ttcagcccca gcccccattt cgccagcgcc agcccggcac cggaatattc tgtcagcggg
    15721 ccttcctgta attcctgttc tgcttcagca acatcaaagg gaatttttcc catctcaatg
    15781 aagcaggcaa aaccacaggc cagtaacgcc agtacggtgg ttagcggcga gttccagccc
    15841 gttgccagcg tattgctgat catctcgata tgcgtggaac ctgctatcag cgccaatacc
    15901 agcagtgaga gaatgagcat tggttcgacc agaatgccga gcgtcaactc gcgactggca
    15961 ccgactcccg caaacgggct tccggtatcc agcccggaaa gagcaaagaa aaaacgaaac
    16021 agggcaagaa gatagataag ggtgatcaga tcgccgccgc ccgcaaaagg ggaaacggta
    16081 ataaatagtg gtagggccat cgccagcacc agcatgctgc tgattaatac ccacggcatc
    16141 aggcggaaca tcagacctga agatataggc gcaacttcct ggcgtttaaa cagtttgtgg
    16201 atatcgcgat aatcctgcca gatccccggc ccgcggcggg agtgcatacg cgcgcgtatc
    16261 tgccgggaaa tacccgtaaa aagcggggtt agcatcagca gaatcacggc ctgtgctaac
    16321 gcaaaaatga ccagatatcc gtcgcaaaga gtttgtctca tggtgatttc cttagacggc
    16381 aatagcgatt agcagcacaa ccagtgcggc gaccacatac aggcaataga gacgaaagtc
    16441 gccgctttgc agatgctgta tttctttggc cagccgttgg gtggcgctca cgatggggcg
    16501 gatcacccgc tcatcccaga agggttctgt gctctgagcc ctggcggtga cgtgcgcaag
    16561 acctttattt agcctcagcg tagggtcgag ctgttgtcgt agacgaaata gcgcagagaa
    16621 gaccacacgc agcggctgca tcacgccatt gcctgacggg gccatcgcat tttcccagcc
    16681 gtagccgcat gcccaggcat ctcctgtgcg acgaaacgct gcgcggcgcg aacgacacca
    16741 cagccagtaa aggccaggca gcaaaggcat cgccagtagt aacagcaaca gtaatgaggg
    16801 ggtgacctgt gtatgaaacg tgccgggtac aagtgctatt ccgctggcga cagtgacggg
    16861 aggggtattg gtaaacgcat gggcaatatg cattatcttc ggtgccagcc aactggcact
    16921 aagcgcaatt aatacgcaga gtgccgcgag cagtagcatg gcgacgatca tcgtatttgg
    16981 cacttcctgt gcctcttcag cgtgtgtact gcgcggcgca ccacagaaag taataccgta
    17041 cattttgacg aagcacatta ctgccagccc accagtgact gccagcatca ccatagcaat
    17101 aggacccgca agttgtagcg ctacggcttc cacacgactt agtgagaaca gcgactgcca
    17161 ggtgtaccat tcgctgataa aaccattcag cggaggaagg gctgatatcg cgaggcaacc
    17221 aatcaggcat gctgcggctg tccacggcat ccgtttcgcc agtgccccca ttttttccat
    17281 gtcgtgggta tgcaaacgcg aaataatcgc gcccgcgccg agaaatagca gccctttgaa
    17341 cagcgcatgg ttgagcagat gaaacagtgc gccgagcagt ccaaccacgg tgagcagcgg
    17401 gtcgtgtagt gacagaccga ccatcgccac accgactgcc agcaaaataa tgccgacgtt
    17461 ttcgacggta ctccaggcca gcagccgttt gatatcctgt tccgccagcg catacagcac
    17521 gcccaggagc gcggagattg cgccgatcgc catcaccaga atgccccacc acagcggcaa
    17581 acccgtttgc gccagcagat ccatcgccac tttcaggatg ccgaaaatac cgattttgac
    17641 cattacgcca gacatcaacg ccgaagcgtg cgatggtgcg gcagggtgag cgcgcggcaa
    17701 ccagctgtgc aacggcatca tcccggcttt cgcgccaaaa ccgaaaaagg ccagcaggaa
    17761 caccgccgac gccagccccg gagaaagtga aagcgtgcgg aaactggcaa aatctaggct
    17821 gccgctttcg cgccccatca gcaagaaggc gatcataatc agcaccgatc cggcgtgggc
    17881 gataaaaaag tagagcatgc cagcgttgat cgacgtttta tcctgcctgg caatgaccag
    17941 aaaccaggaa gacagcgaca tcatttcaaa cagcacgatg aaccaaaaag cgttgtccat
    18001 caccagcagg gcaaccatcg atgcgatgaa aatattcata aagaagccca tcgccgccgc
    18061 gcctttgccc tcgtattcgc gcatataagt caatgaatag agcgaacaaa ccaccaccag
    18121 caaggagata accagcacca taaatgccga gaggctatcc attcgcaggg ttaaatcggc
    18181 aaagtcaaaa ggggtggcaa aacgggcaac taaagttacg ccttcatgta attgcgtaat
    18241 tccgctgatg atgccaatca cgccgcccac cagcgatgtg atgccggaaa gcttaatagc
    18301 cagtctgtcc agaccgagta aaaacagcga agccagactg gcaaacagat agaggatcag
    18361 cgaccaggtt aataattgca gggcatccat tagcgttgct cctgagtgag ggaaagggga
    18421 tcctccctgc cattggcaaa ccagcttgcg gcaaggcgct gtttttcttt catctgacgt
    18481 tgcaggctat cgtcggtgat cagccgtaac gcctgattcg ggcaagcgcg aacgcaggca
    18541 ggaccttctg gcaagaaatc acacaggtcg catttcaccg cgacggtctg gacacccggt
    18601 tcccagcgca gcaaagcatg ttgtgttggc gcgttttctt cgccgtcttt gagtgagcct
    18661 tcagcctgaa aaacatattg cgcaggggca ttcaccggac ggcttcctga agcgctgatt
    18721 gcgccaaatg ggcagaccac ggcgcaaagc ttgcagccaa tacagaggct ttcgttgagt
    18781 tggatcgcat catccctctg ggagatggca ttgaccgggc agacctgcac gcaaggggct
    18841 tcctcacagt gatgacacac gacaggggca gtgagtgttg acgtcttcgc cagggccagg
    18901 cgcgggtgtt gctgtaaacc ttgcgtttta tgcacgtccg aacaggcagc gagacaggta
    18961 ttacatcctg tacaccacag tggttcggcc accacaaagc ggttcatgtc ggctccttga
    19021 aaatgatatg aaacattgca tttcataatg cataaaggat gccagtatga ttaatttaat
    19081 atattcaata agttatgaat taactgaatt ctgaacggta caaaaatgat ggtcattaac
    19141 gttcagcaaa aaatggattc gacacccgtg tcgcagggga aagtgagaat gtgatcttga
    19201 tcacgataag tgaggtgtgc tgaccgcaag cgcagccagc acggaatgga gcaaagtaat
    19261 caggcgtgtt ctttcagcca gttcagcaca acgtcgtggt gattgctggt tttgaaatcg
    19321 tcaaagacat gttcgatttt gccatcagcg tcaatcagga agctgatgcg atgaatacca
    19381 tcgtaggttt tgcccatgaa ggacttttcc ccccagacgc cgaattgctc gcacacctgg
    19441 tggtcctcat cagacaggag cgtaaagtta agcagctctt tttccgcaaa acgggagagt
    19501 ttttcgggtt tatcggtgct gatacccagc acatcaacgc ccgctttttt caactcatcc
    19561 atgttatcgc gtaagccgca ggcctgtacg gtacagccgg gggtcatggc tttcgggtag
    19621 aaataaacca gaacacgctg tccctggaag tcggtcaaat taacttgttc tccgtcttga
    19681 tccggcaagc taaatttcgg tgcgatatca ccggctttca gtggattcat tacttaactc
    19741 catcctgttc atcatgttgg gaataattga cgacgttaat actgccttgt gcattgagtt
    19801 ctgtacatag ggctttgaac gcttgctcaa tatttgctgc gtccgcagat gcggggctgt
    19861 gggcagtaat ctgaatatgc aactgcgcag cccgttcatt ttcagcaggt tgtgtgcgcg
    19921 acaccagctc cgcaatgttc atatgatgcg cgtcgaaaag tgctgtgaag cgttcaatta
    19981 aatgcgggga gtctgccaca tcgacctgaa cccagacaga tgctggcatt ggcggacgcg
    20041 gacgcgccgt cgtgcgcttc atcacgatta aaagatccag ttcggcacct ttcaacggta
    20101 acgttgattc aatcagagta atggcattcc atgaaccgga aagcagcatg ataaacgtga
    20161 actcttctcc cagcatcgcc aggcgactgt cttcaatatt acagccgcaa ctactgacat
    20221 gacgggtgat ggtgttcaca attccagggc gatcggcacc caacgcagtg atcaccagat
    20281 aatgttgcga tgacagtgtc aaactggtta ttcctttaag gggtgagttg ttcttaagga
    20341 aagcataaaa aaaacatgca tacaacaatc agaacggttc tgtctgcttg cttttaatgc
    20401 cataccaaac gtaccattga gacacttgtt tgcacagagg atggcccatg ttcacgggaa
    20461 gtattgtcgc gattgttact ccgatggatg aaaaaggtaa tgtctgtcgg gctagcttga
    20521 aaaaactgat tgattatcat gtcgccagcg gtacttcggc gatcgtttct gttggcacca
    20581 ctggcgagtc cgctacctta aatcatgacg aacatgctga tgtggtgatg atgacgctgg
    20641 agctggctga cgggcgcatt ccggtgattg ccgggaccgg tgctaacgct actgcggaag
    20701 ccattagcct gacgcagcgc ttcaatgaca gtggtatcgt cggctgcctg acggtaaccc
    20761 cttactacaa tcgtccgtcg caagaaggtt tgtatcagca tttcaaagcc atcgctgagc
    20821 atactgacct gccgcaaatt ctgtataatg tgccgtcccg tactggctgc gatctgctcc
    20881 cggaaacggt gggccgtctg gcgaaagtaa aaaatattat cggaatcaaa gaggcaacag
    20941 ggaacttaac gcgtgtaaac cagatcaaag agctggtttc agatgatttt gttctgctga
    21001 gcggcgatga tgcgagcgcg ctggacttca tgcaattagg cggtcatggg gttatttccg
    21061 ttacggctaa cgtcgcagcg cgtgatatgg cccagatgtg caaactggca gcagaagggc
    21121 attttgccga ggcacgcgtt attaatcagc gtctgatgcc attacacaac aaactatttg
    21181 tcgaacccaa tccaatcccg gtgaaatggg catgtaagga actgggtctt gtggcgaccg
    21241 atacgctgcg cctgccaatg acaccaatca ccgacagtgg tcgtgagacg gtcagagcgg
    21301 cgcttaagca tgccggtttg ctgtaaagtt tagggagatt tgatggctta ctctgttcat
    21361 aagtcgcgcc tggcaacggt tgcgggtgtt tcgcttgttt tattactcgc tgcctgtagt
    21421 tctgactcac gctataagcg tcaggtcagt ggtgatgaag cctacctgga agcggcaccg
    21481 cttgcggagc ttcatgcccc ggctggaatg attttgccgg tgacctccgg tgattatgca
    21541 atcccggtga ccaacggtag tggtgctgtc ggtaaggcgc tggacattcg tccaccagcc
    21601 cagccgctgg cactggtttc tggcgcgcgt acccagttca cgggcgatac cgcttcattg
    21661 ctggtggaaa atggtcgtgg caatactctg tggccgcagg tggttagcgt gctgcaggcg
    21721 aaaaactaca ccatcaccca acgtgatgat gctggtcaga cactgaccac cgattgggta
    21781 caatggaacc gtctggacga agacgagcag tatcgtggtc gttatcaaat ctctgttaag
    21841 ccgcagggtt atcagcaggc ggttacggtt aaactgctga acctggaaca ggcgggcaaa
    21901 ccggttgcag acgcggcttc catgcagcgt tacagcacgg agatgatgaa cgttatttcc
    21961 gctggtctgg ataaatctgc cactgacgcc gcgaacgctg cgcaaaatcg tgcctccacc
    22021 actatggacg tacaaagtgc agctgatgac accggtttac caatgctggt cgtacgcggg
    22081 ccgttcaatg tggtttggca acgtctgcca gcggcgctgg aaaaagtggg catgaaagtg
    22141 accgacagca cccgttcgca gggcaacatg gccgtaactt ataagccgct gtctgacagc
    22201 gactggcagg aactgggcgc gagcgatcca ggcctggcat ctggtgacta taaactgcag
    22261 gttggcgatt tagataaccg cagcagccta cagttcatcg atccgaaagg tcatactctg
    22321 actcagagtc agaacgacgc gctggtagct gtcttccagg ctgcgtttag caagtaaaaa
    22381 tacagggctg gaatcatccg gccctttttt ctgatatgat acgcaaacgt gtgcgtcggc
    22441 aggaaaacgc gattttagcg gtaattcgca cgaaatttgt ttgtcggacg tagttcggat
    22501 aaggcgttca cgccgcatcc gacaaaacat ccggcacacc agacagcaaa agattttaaa
    22561 acgttaattc acacccagga gtgataaaga tgcaaaagca agctgagttg tatcgtggta
    22621 aagcgaaaac cgtatacagc acggaaaacc cggacctgtt ggtgctcgaa ttccgcaatg
    22681 atacgtcagc aggggatggc gcgcgcattg agcagtttga tcgcaaaggt atggtgaaca
    22741 acaagttcaa ctacttcatt atgagcaaac tggctgaagc gggtatcccg actcaaatgg
    22801 agcgtctgct ctccgatacc gaatgtctgg tgaaaaagct ggatatggtg ccggttgagt
    22861 gtgtcgtgcg taaccgtgct gctggctctc tggtgaaacg tcttggaatc gaagaaggta
    22921 ttgagctgaa cccgccgctg ttcgatctgt tcctgaaaaa cgacgccatg cacgatccga
    22981 tggtcaacga atcttactgc gaaacctttg gctgggtaag caaagagaac ctggcacgca
    23041 tgaaagagct gacctacaaa gcgaacgacg tgctgaaaaa actgttcgat gatgctggtc
    23101 tgattctggt cgacttcaag ctggagtttg gtctgtacaa aggtgaagtg gtactgggcg
    23161 atgagttctc cccggacggt agccgcctgt gggacaaaga aacgctggag aaaatggaca
    23221 aagaccgttt ccgccagagc ctcggtggcc tgatcgaggc ctatgaagcc gtcgcccgcc
    23281 gcctgggtgt acagctggac tgatttttct gttcatcatc ttgccgtgct gctggcacgg
    23341 caagacaacc attctcgtaa gatgtgcatt gaacgctcat tcctccgcca tttcatcccg
    23401 ttttcctgtg gtaatcttcc tctgaaccac ctacgatcat ttctataatg aatatattgt
    23461 tgaggtatct atgcgttggc aagggcgacg tgaaagtgac aatgttgaag acaggcgcaa
    23521 tagctccggc ggtccgtcaa tgggcggtcc cggtttccgt ctgccgagcg gtaaaggcgg
    23581 gctgatttta ctgatagtcg tgctggttgc aggctactat ggcgttgatt taaccgggtt
    23641 gatgaccggg cagccggttt ctcaacagca atcaacgcgg tcaattagcc caaatgaaga
    23701 cgaagccgca aaattcacct cggtgattct ggcaaccacg gaagacacct ggggacaaca
    23761 gttcgagaag atgggtaaga cctatcagca accgaagctg gtcatgtacc gtggaatgac
    23821 gcgtaccgga tgcggggcgg gccagtccat aatggggccg ttctattgcc cggcggatgg
    23881 tacggtttat atcgatctct ccttctatga tgacatgaaa gacaaacttg gcgcggatgg
    23941 cgattttgcc caggggtacg ttatcgccca tgaagtcggt catcatgtgc agaaactgtt
    24001 aggcatcgag ccgaaagttc gtcaactgca acaaaacgcg acgcaggcgg aagtgaaccg
    24061 cttatctgtg cgtatggaac tccaggccga ctgttttgcc ggtgtctggg ggcatagcat
    24121 gcagcagcaa ggcgttctgg aaaccggcga tctggaagag gcgctgaacg cggcgcaggc
    24181 catcggcgat gaccgtttac aacagcaaag tcaggggcga gtagtaccag acagtttcac
    24241 tcatggcact tctcagcaac gctacagctg gtttaaacgt ggtttcgaca gcggcgatcc
    24301 ggcacaatgc aatacttttg gtaaaagcat ttaacttccg gggcagggat ggctgaactg
    24361 actgcgcttc acacattaac agcgcaaatg aaacgtgaag ggatccgccg cttgctggtg
    24421 ttgagcgggg aagagggttg gtgttttgat catgcgctta agttacgtga tgccttacct
    24481 ggcgactggc tgtggatttc gccgcagcca gatgctgaaa accactgttc tccctcggca
    24541 ctacaaactt tacttgggcg cgagttccgg catgcggtat tcgacgcccg ccacggcttt
    24601 gatgccgctg cctttgccgc acttagcgga acgttgaaag cgggaagctg gctggttttg
    24661 ttactccctg tatgggaaga gtgggaaaac caacctgatg ccgactcgct gcgctggagt
    24721 gattgccctg accctattgc gacgccgcat tttgtccagc atttcaaacg cgtacttacg
    24781 gcggataacg acgctatctt ctggcggcaa aaccagccgt tctcgttggc gcattttact
    24841 ccccgtactg actggcaccc cgcgaccggc gcaccacaac cagaacaaca gcaaatctta
    24901 cagcagctac tgaccatgcc gtcgggcgtg gcagcggtaa ctgctgcgcg tgggcgcggt
    24961 aaatcggcgc tggcagggca actcatttct cgtattgcgg gtagtgcgat tatcactgcg
    25021 cccgcaaaag cggcaacgga tgtactggca caatttgcgg gcgagaagtt tcgctttatt
    25081 gcgcctgatg ccttgttagc cagcgatgag caagccgact ggctggtggt cgatgaagcc
    25141 gcagccatac ctgcgccgtt gttgcatcaa ctggtatcgc gttttcctcg aacgttgtta
    25201 accactacgg tgcagggcta cgaaggtacc ggacgtggtt ttttgctgaa attttgcgct
    25261 cgctttccgc atttacaccg ttttgaactg caacagccga tccgctgggc acagggatgc
    25321 ccgctggaaa aaatggttag tgaggcactg gtttttaacg atgaaaactt cacccataca
    25381 ccacaaggca atatcgtcat ttccgcattt gaacaaacgt tatggcgaag cgagccagaa
    25441 acgccgttaa aggtttatca gttattgtct ggtgcgcact accggacttc gccgctggat
    25501 ttacgccgca tgatggatgc accagggcaa cattttttac aggcggctgg cgaaaacgag
    25561 attgccggag cgctgtggct ggtggatgag gggggattat ctcaagaact cagtcaggcg
    25621 gtatgggcag gttttcgtcg cccgcggggt aatctggtgg cccagtcgct ggcggcgcac
    25681 ggcagcaatc cactggcggc gacattgcgt ggacggcggg tcagccggat agcagttcat
    25741 ccggcgcgtc agcgcgaagg cgttgggcaa cagctcattg ccagcgcttt gcaatatagg
    25801 cctggcctcg actatctttc ggtgagtttt ggttacaccg gggagttatg gcgtttctgg
    25861 caacgctgcg gttttgtgct ggtgcgaatg ggtaatcatc gtgaagccag cagcggttgc
    25921 tatacggcga tggcgctgtt accgatgagt gatgcgggta aacagctggc tgaacgtgag
    25981 cattaccgtt tacgtcgcga tgcgcaagct ctcgcgcagt ggaatggcga aacactccct
    26041 gttgatccac taaacaatgc cgtcctttct gacgacgact ggcttgaact ggccggtttt
    26101 gctttcgctc atcgtccgct attaacatcg ttaggttgct tattgcgtct gctacaaacc
    26161 agtgaactgg cattaccggc gctgcgtggg cgtttacaga aaaacgtcag cgacgcgcag
    26221 ttatgtacca cacttaaact ttcaggccgc aagatgttac tggtccgtca gcgggaagag
    26281 gccgcacagg cgctgttcgc acttaatgat gttcgcactg agcgtctgcg cgatcgcata
    26341 acgcaatggc aattttttca ctgactcctt caattttctc ccatggtcat tgtgagtcag
    26401 cggcgtaaaa ttaatgacat cagttaagga gttcgccatg aaacatgacc attttgttgt
    26461 tcaaagcccg gataaacctg cgcaacagtt gttactgctt ttccacggtg tcggggataa
    26521 cccggtggca atgggggaaa taggtagctg gtttgcaccg ttgttcccgg atgcgctggt
    26581 ggtgagtgtc ggtggcgcgg agccgagcgg taatccggcg gggcgtcagt ggttttcggt
    26641 gcagggtatt acggaagata atcgccaggc gcgtgtggat gccatcatgc cgacgtttat
    26701 tgaaacggtg cgctactggc agaaacagag cggagtaggg gctaacgcca cggcgctaat
    26761 cggtttctct caaggcgcga ttatggcgct ggagagcatt aaagccgaac cgggccttgc
    26821 ttcacgcgtc attgctttta acggacgcta tgcgagcctg ccggaaaccg cgtctacagc
    26881 cacgacaatt cacttgattc atggcggtga agatccggtt atcgatctgg cgcatgcagt
    26941 tgcggcgcag gaagcgttaa tcagtgccgg tggtgacgtg acgctggata tcgtggagga
    27001 tttaggccat gcgatcgaca atcgcagtat gcaatttgcc ctggatcact tgcgttatac
    27061 cattccgaaa cattatttcg acgaagcgct aagcggcggt aagcctggcg atgatgatgt
    27121 gattgaaatg atgtaataat agccgggtag gcctgataag accgtatcag gcatcatggc
    27181 cggatgcggc gttaacgcct tatccggcca cttagcttac ttcttcggtt gatccttttt
    27241 cggccagtcg tcgtcatcgt cccacttatc gttgaaatca cgatgcgggg gaagctccgg
    27301 cttgttggcg agaaatttct tatggtcgac gcgcttgaga tctttaatca cgttcagcag
    27361 gacgcctacc aaaaagacaa tcaccagaat ccaccaatat ttagccagcc agtccatgct
    27421 tatttcctct tgcagaacca ctcatcacgc gacgagctgt tccatgatac gttgatacat
    27481 acgggcaagt agctgcaggt cggcagcgtt cacacattca ttaattttat gaatagtggc
    27541 attgaccggc ccgagttcca ccacctgcgc ccccatgcgg gcaataaagc gcccgtcgga
    27601 cgttccgcct gtggtcagta gctgcggttt aatttcatta tagtgctcaa ccgcgttaac
    27661 gaccgcatcc accagtttac cgcgcgcggt caaaaatggc tgcccggaaa gccaccaatc
    27721 caccgtatag cgcagttgat gtttttcaag cagggcaagc acctgcgctt tgatcatctc
    27781 atcagtcagt tcggtgctga agcggaagtt aaactgcaca aacagttcac ccggaataac
    27841 gttgttactg cccgttcccg cctgaatatt ggcaatctgc atactggtcg ccgggaagaa
    27901 ttcattgccc tgatcccact caatagccac taattcatta aggaaaggtg ctgcgcgatg
    27961 taccggattg tcagccagat gtgggtaggc aacatgcccc tgaacgccat gaatggttag
    28021 gttgcaggtt aacgatccac gacgaccatt tttcaccaca tcacctacca cttcgatact
    28081 cgacggttcg ccgaccaggc agtaatcgag acgctcatta cgtgccatta acgcttcgac
    28141 ggcttttacc gtaccattgt gggcactggc ttcttcatca gaggtgatca gaaatgccag
    28201 tcgccctgta tggttgggat gttgtgcgac aaaacgttct gcagctacca ccatcgccgc
    28261 cagcgagcct ttcatatctg ccgcaccgcg cccgaataac atgccgtcac gaatggttgg
    28321 ttcaaacggc ggattgatcc aacgatcggc gtcgccaggc ggcaccacgt cggtatgccc
    28381 ggcaaaggcc aatgtttcac cttgcccacg ccatgcccaa aaattctgcg tatcggcaaa
    28441 gtccatgcgt tcaacggtaa aaccgatcgc ctgcaaacgt tcaatcaaca aagcctggca
    28501 tcctgcgtca tcaggactca gggaagggcg gcgaataagc tgttgtgtca gctcaataac
    28561 cgggcacgac atagactaca cctcatggaa aaatttctga taactggaat cactgaaacc
    28621 cagcagcata ggcttaccag gcgcgcagag caatggacgt ttgataattg caggcatttc
    28681 agtcattaat gccgccgcag aggccgcatc ggtgattata ttgcgggtgg tttcgtccag
    28741 tttacgccag gttgtaccac gggtgttgag taacgcttcc cagcctaatt cgttgataaa
    28801 accgttcaat aattcgctgt ccagcccatc gacgcggtaa tcatgaaaac gatagtcgat
    28861 gttattggct tccagccaac ggcgagcctt tttaatggtg tcacaatttt tgatgccgta
    28921 aagtgtaacc ataatgaatc ctttttcgat ttatgagaat aaatactcat ttaagagtga
    28981 aaacatttt
//
LOCUS       DBFIFI010000041        28684 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.041, whole genome shotgun sequence.
ACCESSION   DBFIFI010000041 DBFIFI010000000
VERSION     DBFIFI010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28684
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.041"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..649
                     /locus_tag="N7B39_001707"
     CDS             <1..649
                     /locus_tag="N7B39_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGU2421709.1"
                     /translation="AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFG
                     INMNNVTNSSIDFTPLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRL
                     LIEQQLADKPWKLDWFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVE
                     PVIRRTLGIIRRKDAVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            complement(690..1685)
                     /gene="pgl"
                     /locus_tag="N7B39_001708"
     CDS             complement(690..1685)
                     /gene="pgl"
                     /locus_tag="N7B39_001708"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HGU2421710.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNIDHRGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            1840..2658
                     /gene="ybhA"
                     /locus_tag="N7B39_001709"
     CDS             1840..2658
                     /gene="ybhA"
                     /locus_tag="N7B39_001709"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HGU2421711.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDEMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(2659..3717)
                     /gene="modC"
                     /locus_tag="N7B39_001710"
     CDS             complement(2659..3717)
                     /gene="modC"
                     /locus_tag="N7B39_001710"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HGU2421712.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(3720..4409)
                     /gene="modB"
                     /locus_tag="N7B39_001711"
     CDS             complement(3720..4409)
                     /gene="modB"
                     /locus_tag="N7B39_001711"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HGU2421713.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(4409..5182)
                     /gene="modA"
                     /locus_tag="N7B39_001712"
     CDS             complement(4409..5182)
                     /gene="modA"
                     /locus_tag="N7B39_001712"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU2421714.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(5349..5498)
                     /gene="acrZ"
                     /locus_tag="N7B39_001713"
     CDS             complement(5349..5498)
                     /gene="acrZ"
                     /locus_tag="N7B39_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HGU2421715.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            5627..6415
                     /gene="modE"
                     /locus_tag="N7B39_001714"
     CDS             5627..6415
                     /gene="modE"
                     /locus_tag="N7B39_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HGU2421716.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            6483..7955
                     /gene="modF"
                     /locus_tag="N7B39_001715"
     CDS             6483..7955
                     /gene="modF"
                     /locus_tag="N7B39_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HGU2421717.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITPLLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYHYALTKIN"
     gene            8217..9233
                     /gene="galE"
                     /locus_tag="N7B39_001716"
     CDS             8217..9233
                     /gene="galE"
                     /locus_tag="N7B39_001716"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HGU2421718.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SRACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            9243..10289
                     /gene="galT"
                     /locus_tag="N7B39_001717"
     CDS             9243..10289
                     /gene="galT"
                     /locus_tag="N7B39_001717"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HGU2421719.1"
                     /translation="MTQFNPVDHPHRRYNQLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            10293..11441
                     /gene="galK"
                     /locus_tag="N7B39_001718"
     CDS             10293..11441
                     /gene="galK"
                     /locus_tag="N7B39_001718"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HGU2421720.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            11435..12475
                     /gene="galM"
                     /locus_tag="N7B39_001719"
     CDS             11435..12475
                     /gene="galM"
                     /locus_tag="N7B39_001719"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HGU2421721.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGKQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNYLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLHPGEE
                     YSSLTEYQFIAE"
     gene            12677..13429
                     /gene="gpmA"
                     /locus_tag="N7B39_001720"
     CDS             12677..13429
                     /gene="gpmA"
                     /locus_tag="N7B39_001720"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HGU2421722.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(13595..14647)
                     /gene="aroG"
                     /locus_tag="N7B39_001721"
     CDS             complement(13595..14647)
                     /gene="aroG"
                     /locus_tag="N7B39_001721"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HGU2421723.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            14963..15343
                     /gene="ybgS"
                     /locus_tag="N7B39_001722"
     CDS             14963..15343
                     /gene="ybgS"
                     /locus_tag="N7B39_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HGU2421724.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHPDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            15457..16398
                     /gene="zitB"
                     /locus_tag="N7B39_001723"
     CDS             15457..16398
                     /gene="zitB"
                     /locus_tag="N7B39_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HGU2421725.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVIGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(16395..17114)
                     /gene="pnuC"
                     /locus_tag="N7B39_001724"
     CDS             complement(16395..17114)
                     /gene="pnuC"
                     /locus_tag="N7B39_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HGU2421726.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVIIMQALGLQVAMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(17152..18195)
                     /gene="nadA"
                     /locus_tag="N7B39_001725"
     CDS             complement(17152..18195)
                     /gene="nadA"
                     /locus_tag="N7B39_001725"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HGU2421727.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKKTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNYEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(18473..18548)
                     /locus_tag="N7B39_001726"
     tRNA            complement(18473..18548)
                     /locus_tag="N7B39_001726"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18513..18515),aa:Lys,seq:ttt)
     gene            complement(18695..18770)
                     /locus_tag="N7B39_001727"
     tRNA            complement(18695..18770)
                     /locus_tag="N7B39_001727"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18735..18737),aa:Lys,seq:ttt)
     gene            complement(18774..18849)
                     /locus_tag="N7B39_001728"
     tRNA            complement(18774..18849)
                     /locus_tag="N7B39_001728"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18814..18816),aa:Val,seq:tac)
     gene            complement(18901..18976)
                     /locus_tag="N7B39_001729"
     tRNA            complement(18901..18976)
                     /locus_tag="N7B39_001729"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18941..18943),aa:Lys,seq:ttt)
     gene            complement(18979..19054)
                     /locus_tag="N7B39_001730"
     tRNA            complement(18979..19054)
                     /locus_tag="N7B39_001730"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19019..19021),aa:Val,seq:tac)
     gene            complement(19090..19165)
                     /locus_tag="N7B39_001731"
     tRNA            complement(19090..19165)
                     /locus_tag="N7B39_001731"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19130..19132),aa:Lys,seq:ttt)
     gene            complement(19330..20121)
                     /gene="cpoB"
                     /locus_tag="N7B39_001732"
     CDS             complement(19330..20121)
                     /gene="cpoB"
                     /locus_tag="N7B39_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HGU2421728.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVIQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSGDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(20131..20652)
                     /gene="pal"
                     /locus_tag="N7B39_001733"
     CDS             complement(20131..20652)
                     /gene="pal"
                     /locus_tag="N7B39_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HGU2421729.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(20687..21979)
                     /gene="tolB"
                     /locus_tag="N7B39_001734"
     CDS             complement(20687..21979)
                     /gene="tolB"
                     /locus_tag="N7B39_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HGU2421730.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(22112..23386)
                     /gene="tolA"
                     /locus_tag="N7B39_001735"
     CDS             complement(22112..23386)
                     /gene="tolA"
                     /locus_tag="N7B39_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HGU2421731.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADDKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAAAEKAAA
                     DKKAAEKAAADKKAAEKAAAEKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADK
                     KAAAAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNG
                     ASGADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQ
                     AALAAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            complement(23451..23879)
                     /gene="tolR"
                     /locus_tag="N7B39_001736"
     CDS             complement(23451..23879)
                     /gene="tolR"
                     /locus_tag="N7B39_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HGU2421732.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(23883..24575)
                     /gene="tolQ"
                     /locus_tag="N7B39_001737"
     CDS             complement(23883..24575)
                     /gene="tolQ"
                     /locus_tag="N7B39_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HGU2421733.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(24572..24976)
                     /gene="ybgC"
                     /locus_tag="N7B39_001738"
     CDS             complement(24572..24976)
                     /gene="ybgC"
                     /locus_tag="N7B39_001738"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HGU2421734.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(25126..25419)
                     /gene="ybgE"
                     /locus_tag="N7B39_001739"
     CDS             complement(25126..25419)
                     /gene="ybgE"
                     /locus_tag="N7B39_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HGU2421735.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(25419..25532)
                     /gene="cydX"
                     /locus_tag="N7B39_001740"
     CDS             complement(25419..25532)
                     /gene="cydX"
                     /locus_tag="N7B39_001740"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HGU2421736.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(25547..26686)
                     /gene="cydB"
                     /locus_tag="N7B39_001741"
     CDS             complement(25547..26686)
                     /gene="cydB"
                     /locus_tag="N7B39_001741"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HGU2421737.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(26702..28270)
                     /gene="cydA"
                     /locus_tag="N7B39_001742"
     CDS             complement(26702..28270)
                     /gene="cydA"
                     /locus_tag="N7B39_001742"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HGU2421738.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT         7139 a         7600 c         7052 g         6893 t
ORIGIN      
        1 cgcagtattt tattttttac cgctggcaat cggtaaattt aatgagctgt atcccaatat
       61 taaagtgcgg attctggaac aaggcacgaa taattgcatg gaatcggtgc tgtgtaacga
      121 atcagacttt ggcataaaca tgaacaacgt cacaaattca tccattgatt ttaccccact
      181 ggtcaacgag ccgttcgtgc tggcctgtcg gcgtgaccat ccattggcta aaaagcagct
      241 tgtagaatgg caggagttag tgggttacaa aatgattggc gtacgttcct ccagtggcaa
      301 ccgattgttg atagaacaac agctagccga caagccctgg aagctggact ggttttacga
      361 agtgcgtcat ctttcgacgt cattaggact ggtagaggcg ggactgggaa tttcggcgct
      421 tcctggtctg gcaatgcccc atgcgcccta ttcttctatt attggtatcc cactggtgga
      481 acccgttata cgtcggacat tggggattat tcgccgcaaa gatgccgtgc tttctccggc
      541 agcagaacgc ttttttgcct tgctgattaa tttgtggact gacgataaag acaatttgtg
      601 gaccaatatc gtcgaacgcc agagacatgc gttacaggag ataggttaat acaccggcgc
      661 aggagagact gcgccgggta aatcagcggt tagtgtgcgt taaccaccac ccacattggt
      721 ccctgcccga ccgcgtagcg gcctttttca tgcagtagcc cctgctcacc aacaatttcg
      781 tataccgaga tgtggtgcga tttttgcccg gcggcaatca gatacttacc acggtgatcg
      841 atattaaagc cgcgcggctg ggtttcagtt ggctggaagc cctctttact caacacgctg
      901 ccatcttccg aaacgctgaa gacagtaatc aggctggcgg tacggtcaca ggcatataag
      961 tgacgaccat ccggggtaat atgaatatca gccgcccaac gggtgtcgga gaagttttcc
     1021 ggcatcatat ccagcgtctg gacacattcg atattgccgt gcggatcttt cagttcccag
     1081 acatccactg agctgtttaa ctcattgacg caatatgcat attgttcgtt tggatggaat
     1141 accatatgac gcgggccggc cccttcaacg gtagtcactt ccgcagggtc ctgcgccacc
     1201 agatgaccat catcgctgac cgtaaacagg caaatgcgat cctgctttaa tgccggaacc
     1261 cacagcgtac ggttgtccgg tgagatattg gcggaatggc aaccgtccag cccctcgacc
     1321 acatcgacga cgccaactgg caggccatct tccagacgcg ttacgctcac gttacccgca
     1381 ttgtaagaac ctacaaagac aaactgcccc tggtgatcgg tggaaatatg cgtcggacta
     1441 cccggcagcg cagactctgc ggcaaaggtc agtgcgccat cgtccggggc gatacgatac
     1501 gccaggacgc gaaactcagg gcgaacacca acatagagat aacgtttgtc cgggctgacc
     1561 accatcggct gcacctgccc cggcacatcg acaacctgtg tcagcgtcag tgcgccttca
     1621 tgattcaggt tccagacgtg aatttgctgg ctctcagggc tggcgatata aactgtttgc
     1681 ttcatgaatg ctcctttgca tttagcaact gactgtaaca gctaaaatta gtcgcttttg
     1741 gcggtgaatg ctgaattaaa acgcagaatt ttgtccgata gtggtatcgg tgtaccattc
     1801 gcgcagaaga aaattcttaa ccttaatctg gaatacgcca tgaccacacg cgtgattgct
     1861 ctcgacttag acggcacctt attgaccccg aaaaagaccc tgctaccttc atcgatagaa
     1921 gccctggccc gcgctcgcga agcaggttat caattaatca tcgtcacagg tcgccatcac
     1981 gtcgctattc atccttttta tcaggcgctg gcgctggata cacctgctat ttgctgtaat
     2041 ggcacctatt tgtatgatta tcatgcaaaa accgtgctgg aagcggaccc aatgcccgtt
     2101 aataaagccc tacaactcat tgagatgctg aatgaacacc acattcacgg tctgatgtat
     2161 gtcgatgacg agatggttta tgagcacccg accgggcatg tcattcgcac gtctaactgg
     2221 gcgcaaaccc tgccgccgga acagcgtccg actttcacac aagtcgcttc tctggctgaa
     2281 acggcgcaac aagttaacgc cgtatggaag ttcgccctca cgcacgatga cctgccgcaa
     2341 ttgcagcatt ttggtaagca tgtcgaacat gaactgggac tggagtgtga atggtcctgg
     2401 cacgatcagg ttgatattgc acgcggcggc aacagcaaag gtaaacgttt gacgaaatgg
     2461 gttgaggcgc aaggctggtc gatggaaaac gtcgtggcgt tcggcgataa ctttaatgat
     2521 atcagtatgc tggaggccgc tggtacaggc gtggcgatgg gcaacgccga tgacgcggta
     2581 aaagcgcgcg ccaacattgt gattggtgat aacaccaccg acagcattgc ccagttcatt
     2641 tatagccacc tgatttaatc aggcggttat cgacacactt ttaatttgcg cgtacagcca
     2701 caggccaggt ttgatcgcca gttcatccct ggcccacggg ctgatacgcg cccacagcgt
     2761 tttaccgccg acttccagtt ccacttccac ctggccgttg tcgtcataac tattaacaac
     2821 ttttgcccgc aatacgttac gaatgctggt ttgctgcggc ggttgtaaaa ccagagaaac
     2881 atcggaagcc tgaatgcgga tacgtagcgc agcttgcagc ggttcgtcca gcttattgac
     2941 ccacaaatgc tgatcgccca gcgccagcgc ggtcatcgcg taatgcggat gatgctcaag
     3001 taccgtcact ttcagaatgc tactttgttg ctctttcggc agccacggat tcatcacgct
     3061 actaccccag acttcttcca gcgcgccaaa ggctttcacc tgaccgtttt ccagtaccat
     3121 cactctgtct gccagatgga ggatctcatc cagcgaatgg ctgacataca acatcgggat
     3181 gttgatttcc cgcgtcagac gttgcagata aggcaacagt tcgcgtttac gcggaatatc
     3241 cagtgacgcc agcggttcat ccagcaacag caattctggt gccgtcagca aagcccgccc
     3301 aatcgctacg cgctgttttt cgcctccgga caggctgcct ggtaaacggt caagcaacgg
     3361 ttcaatgcct aaaagcgcca ccagcttatc gaactgatcg accatgcttt tcgccatgcc
     3421 gtagcgcaga ttgccacgca ctttgtaatg cgggaacagc cgcgcatcct gaaaaacata
     3481 gccaacgcga cgcttttccg gcgtcaggca gatacctttt tcggcatcat ttagtacccg
     3541 accattgagg acaatcctcc ctttttgcgg gcgcgtcagt ccactgatgg cgttaatcag
     3601 cgaagttttt ccggcaccgg agacgccaaa gatagcagtg atgccattgg cgggcagcgt
     3661 ttcattaata gtcaggcaat ggttgcccaa cgtctgggaa aaattcagtt ccagcatgat
     3721 tagcgccccg cccgttcacg gctgattctg gccagccatt ctgaaatcaa cagggagatc
     3781 atcgccagcg caatagaaat aatgcacagt ctcgctgcgc cactttcccc gccgggggtc
     3841 tgaatcaggg tatacatggc agaaggaatg gttcgcgttt caccaggaat gttcgacaca
     3901 aaggtgatgg ttgcgccaaa ttcaccgaga gaacgagcaa aagccagtac cgtaccaaca
     3961 ataattcccg gtaaggtcag cggtaacgtg atagtaaaga aaacgcgcca gcgcccggcc
     4021 cccagtgttc ttgcggcctg ttccagtttg acatcaaccc cttccagcgc cagacgaatt
     4081 gcccgcacca tcagcggaaa cgacatgacg gcggcagcga gaaccgcgcc gcgccagcta
     4141 aaggcgaagg taataccaaa ccagtcatac agacgttcac cgataaatcc gcgccgcccc
     4201 atcgaaacta ataataagta accgacgacc acgggcggta acaccagcgg tagatgcagt
     4261 acgctgtcga gcagagcttt gcccggaaac gtgcaacgca ccagtaacca ggcaaaaaag
     4321 atcccaaacg gcaggctaaa cagcacagcc agggaagaaa ctttcaggct taataaaact
     4381 gcctgccatt ctggatcggt cagtatcatt acttggttgt aaatccgtaa cgtttaaaga
     4441 tttccgctgc ctgcggtccc ttcagataat cataaaatgc tttcactgtc gcattgttat
     4501 gcccttccac aacagcaacc ggatattcca cttttttatg tgaatcttcc gggaaggtgg
     4561 caaccacttt tacccctttg ctagcaactg cgtcagaacc gtaaacaatg cccaacggcg
     4621 cttcattgcg ttcgaccaac gccagcgcgc cacgaacatc ttccgccgga gccagtttcg
     4681 gggagagcgt atcccatgcg cccagttttt gcagtgcttc ttttgcataa atgccagcgg
     4741 gaacatgttc cggatcgcca accgccaggc gaccgccatt cagcagtgaa gtccagttgg
     4801 ttttgctgtc gatggtaaaa tctttctgct cgctggcttt cggtgctaca acgaccaggc
     4861 tattgccgag cagtgtctga cgcgtagctg tatcgatcgc ttttttatca accgcataat
     4921 ccatccattt ctgatcggca gaaataaaca gatccgcagg ggcacccgct tcaatctgac
     4981 gggcgagagt agatgacgaa gcgaaagaag aaaccacatc cacgcctttc tcttttttat
     5041 actgcgtagc aatgtcctgc attgcgttag tcagtgatgc ggcggcgaac accgtgattt
     5101 tcccttcatc tgccagtgca ttgccagcaa cagcgaaaga gagtgccgcc ccagcaaaca
     5161 agttcaacca tttacgagcc attggtaacc ccttaatgta acgttatgta ggcgacaata
     5221 taacgactaa ctcggaattt tcccagcagt tattgctaac ctttgtgagg tagataagaa
     5281 aaaatatcgg caggaaaagc aggaagttga gaaaaagaaa atgcccgact aagcgggcat
     5341 tcagggaatc aatgattttg tccgggctgg tcttttttac caacaccaga aaagatgttg
     5401 aatacttcac caagaccgta aatcagaccc aggatgatgg ccatcacgac aggtaccatg
     5461 attacggcga ataccagact ttttaataac tctaacatgg tcaactccag atatagtcat
     5521 gagactattc taaccgctaa gcacagaaaa gcactcccct tttgtgcggt cagctttgcg
     5581 tggcgttcgt tttccgtcac aataagactt ttgccaggac attgttatgc aggccgaaat
     5641 ccttctcacc cttaagctcc aacaaaaatt attcgccgac ccgcgccgca tttcgctact
     5701 aaaacacatt gcgctttccg gttccattag ccagggagcg aaagatgccg gtatcagcta
     5761 taaaagcgcc tgggatgcca ttaacgagat gaatcagtta agtgagcata ttctggtcga
     5821 gcgcgcaaca ggcggtaaag gtggcggcgg cgcagtactg acccgctatg gtcagcgact
     5881 gattcagctc tatgacttac tggcgcaaat ccagcaaaaa gcctttgatg tgttaagtga
     5941 cgatgacgcc ctgccgctga acagcctgct ggccgcgatc tcacgttttt cactccaaac
     6001 cagcgcccgt aaccagtggt tcggtaccat caccgcccgc gatcatgatg acgttcaaca
     6061 gcatgttgat gtcttactgg ctgatggaaa aacacgcctg aaagtcgcaa ttaccgcaca
     6121 aagcggcgcg cgtctggggc tggatgaagg caaagaagtg ttgatattgc taaaagcgcc
     6181 gtgggtaggc attactcagg acgaagcggt cgcgcaaaac gctgacaacc aattaccggg
     6241 tattattagt catattgagc gcggcgcaga gcagtgcgaa gtattaatgg cgctaccgga
     6301 cgggcaaaca ctgtgcgcca cagtgccggt aaatgaagcg acttctcttc agcaaggaca
     6361 gaatgtcacg gcctacttta atgccgacag cgtgattatc gccacactgt gctaagcgtg
     6421 ttgacaattt gttatgaaac acgtatccct gtcagtaatc gctgcacaaa atgggatata
     6481 aaatgtcatc gttgcaaatt ttgcaaggca cgtttcgtct tagcgacaca aaaacgctgc
     6541 aattgcctca gctaacgtta aacgcgggtg atagttgggc gtttgtcggt tcgaatggaa
     6601 gcgggaaatc ggccctggcc cgcgcgctgg cgggggaact tccgcttttg aaaggtgaac
     6661 ggcaaagcca gttttcccac atcactcgtc tctccttcga gcaattgcaa aagctcgtca
     6721 gcgacgaatg gcagcggaat aacaccgata tgctcggccc tggcgaagat gacaccggac
     6781 gcactacggc tgagattatt caggatgaag taaaggatgc accgcgctgc atgcaactgg
     6841 cacagcagtt cggtattacc cccctcctcg accgacgctt taaatacctt tccactggcg
     6901 agacgcgaaa aaccctgctg tgtcaggcgc tgatgtcgga gcctgacttg ttgattcttg
     6961 atgagccgtt cgatggcctg gatgttgcct cacgtcagca gctggctgag cgactcgcct
     7021 cgttacatca gtccggtatt actctggtac tggtgctcaa tcgcttcgat gagatcccgg
     7081 agtttgtcca gtttgctggc gtgctggcgg attgcacgtt agcggaaact ggcgctaaag
     7141 aggaactgct ccaacaagca ctcgtcgcgc aactggcgca tagtgaacag cttgaaggtg
     7201 tgcaactgcc ggagccggat gaaccttcag cacgtcacgc cttacccgcc aacgaaccgc
     7261 gcattgtgct gaataatggc gtggtttctt ataacgatcg ccccattctt aataacctta
     7321 gctggcaggt gaatccaggt gaacactggc aaattgtcgg gccaaatggc gcgggaaaat
     7381 cgacgttatt aagcctgatt actggcgatc atccgcaagg ttacagcaac gatttgacgc
     7441 ttttcggacg acgtcgtggc agcggcgaaa ccatctggga tatcaaaaag catatcggtt
     7501 acgtcagcag tagtttgcat ctggattacc gggtcagtac tactgtgcgt aatgtgattc
     7561 tttctggcta ttttgattcg attggcattt atcaggccgt ttcggatcgc cagcaaaaac
     7621 tggtgcagca gtggctggat attctcggca ttgataaacg cacggctgac gctccgttcc
     7681 atagtctttc ctggggacag cagcgtctgg cgctgatcgt ccgcgcactg gtgaaacatc
     7741 cgacgttgct tattctcgac gaaccattgc aggggcttga tccgctcaat cgccagctta
     7801 ttcgccgttt tgttgatgtg ctgattagcg aaggtgaaac gcaattgttg tttgtttcgc
     7861 accatgctga agatgcgccg gcttgtatta cccatcgtct ggagttcgtg ccggacggtg
     7921 gcctctatca ttatgcgctg acaaaaataa actaagtcgg taatgctgac tgtgccggat
     7981 gcggcgtaaa cgctttatcc ggcacacggt cagacgcatc caggcatgaa actgcgtctt
     8041 tttttcagat aaaaagcgca atcattcaca atccctctgt tttataatca cttaatcgcg
     8101 cataaaaaac ggctaaattc ttgtgtaaac gattccacta atttattcca tgtcacactt
     8161 ttcgcatctt tgttatgcta tggttatttc ataccataag cctaatggag cgaattatga
     8221 gagttctggt cactggtggt agcggttaca ttggaagtca tacctgtgtg caattactgc
     8281 aaaacggtca tgatgtcatc attcttgata acctctgtaa cagtaagcgc agcgtactgc
     8341 ctgttatcga gcgtttaggc ggcaaacatc cgacgtttgt tgaaggcgat atccgtaacg
     8401 aagcgttgat gaccgagatc ctgcacgatc acgctatcga caccgtgatc cacttcgccg
     8461 ggctgaaagc cgttggcgaa tcggtacaaa aaccgctgga atattacgac aacaatgtca
     8521 acggtactct gcgcctgatt agcgccatgc gcgccgctaa cgtcaaaaac tttattttta
     8581 gctcctccgc caccgtttat ggcgatcagc ccaaaattcc atacgttgaa agcttcccga
     8641 ccggcacacc gcaaagccct tacggcaaaa gcaagctgat ggtggaacag atcctcaccg
     8701 atctgcaaaa agcccagccg gactggagca ttgccctgct gcgctacttc aacccggttg
     8761 gcgcacatcc gtcgggcgat atgggcgaag atccgcaagg cattccgaat aaccttatgc
     8821 catacatcgc ccaggttgct gtaggccgtc gcgactcgct ggcgattttt ggtaacgatt
     8881 atccgaccga agacggtact ggcgtacgcg attacatcca cgtaatggat ctggcggacg
     8941 gtcacgtcgt ggcgatggaa aaactggcga acaagccagg cgtacacatc tacaacctcg
     9001 gtgctggcgt aggcagcagc gtgctggacg tggttaatgc cttcagcaga gcctgcggca
     9061 aaccggttaa ctatcatttt gcaccgcgtc gcgagggcga ccttccggcc tactgggcgg
     9121 acgccagcaa agccgaccgt gaactgaact ggcgcgtaac gcgcacactc gatgaaatgg
     9181 cgcaggacac ctggcactgg cagtcacgcc atccacaggg atatcccgat taaggaacga
     9241 ccatgacgca atttaatccc gttgatcatc cacatcgccg ctacaaccag ctcaccgggc
     9301 aatggattct ggtttcaccg caccgcgcta agcgcccctg gcagggggcg caggaaacgc
     9361 cagccaaaca ggtgttacct gcgcacgatc cagattgctt cctctgcgca ggtaatgtgc
     9421 gggtgacagg cgataaaaac cccgattaca ccgggactta cgttttcact aatgactttg
     9481 cggccttgat gtctgacacg ccagatgcgc cagaaagcaa cgatcctttg atgcgttgcc
     9541 agagcgcgcg cggtaccagc cgggtgatct gcttttcacc ggatcacagt aaaacgctgc
     9601 cagaactgag cgttgcggca ttgacggaaa tcgtcaaaac ctggcaggag caaaccgcag
     9661 aactggggaa aacatacccg tgggtgcagg tctttgaaaa caaaggtgcg gcgatgggct
     9721 gctctaaccc gcatccgcac ggacagattt gggcaaatag cttcctgcct aacgaagctg
     9781 agcgcgaaga ccgcctgcaa aaagaatatt ttgccgagca gaaatcgcca atgctggtgg
     9841 attatgttca gcgcgagctg gcagacggta gccgtaccgt tgtcgaaacc gaacactggt
     9901 tagcagttgt gccttactgg gctgcctggc cgttcgaaac gctacttctg cccaaagccc
     9961 acgttttgcg gatcaccgat ttgaccgacg cccagcgcag cgatttggca ctggcgttga
    10021 aaaagctgac cagtcgttat gacaacctct tccagtgctc cttcccctac tctatgggct
    10081 ggcacggcgc accgtttaat ggcgaagaga atcaacactg gcagctgcac gcgcactttt
    10141 atccgcctct gttgcgctcc gccaccgtac gtaaatttat ggttggttat gaaatgctgg
    10201 cagaaaccca gcgagacctg accgcagaac aggcagcaga gcgtttgcgc gcagtcagcg
    10261 atatccattt tcgcgaatcc ggagtgtaag aaatgagtct gaaagaaaaa acacaatctc
    10321 tgtttgccaa cgcatttggc taccctgcca ctcataccat tcaggcgcct ggccgcgtga
    10381 atttgattgg tgaacacacc gactacaacg acggtttcgt tctgccctgc gcgattgatt
    10441 atcaaaccgt gatcagctgt gcgccacgcg atgaccgtaa agttcgcgta atggcagccg
    10501 attatgaaaa tcagcttgat gagttttccc ttgatgcgcc cattgtcgcg catgaaaact
    10561 atcaatgggc gaactacgtt cgtggcgtgg tgaaacatct gcaactgcgt aacaacagct
    10621 tcggcggtgt ggacatggtg atcagcggca atgtgccgca gggtgccggg ttaagttctt
    10681 ccgcttcact ggaagtcgcg gtcggaaccg tattgcagca gctttatcat ctgccgctgg
    10741 acggcgcaca aatcgcgctt aacggtcagg aagcagaaaa ccagtttgtt ggctgtaact
    10801 gcgggatcat ggatcagcta atttccgcac tcggcaagaa agatcatgcc ttgctgattg
    10861 actgtcgctc actggggacc aaagcagttt ccatgccgaa aggtgtggct gtcgtcatca
    10921 tcaacagtaa cttcaaacgt accctggttg gcagcgaata caacacccgt cgtgaacagt
    10981 gcgaaaccgg tgcgcgtttc ttccagcagc cagctctgcg cgatgtcacc attgaagagt
    11041 tcaacgctgt tgcacatgag ctggacccaa tcgtggcgaa acgcgtgcgt catatcctga
    11101 ctgaaaacgc ccgcaccgtt gaagctgcca gcgcgctgga gcaaggcgac ctgaaacgta
    11161 tgggcgagtt gatggcggag tctcatgcct ctatgcgcga tgatttcgaa atcaccgtgc
    11221 cgcaaattga cactctggta gaaatcgtca aagctgtgat tggcgacaaa ggtggcgtac
    11281 gcatgaccgg cggcggattt ggcggctgta tcgtcgcgct gatcccggaa gagctggtgc
    11341 ctgccgtaca gcaagctgtc gcagaacaat atgaagcaaa aacaggtatt aaagagactt
    11401 tttacgtttg taaaccatca caaggagcag gacagtgctg aacgaaactc ccgcactggc
    11461 acccgatggt cagccgtacc gactgttaac tttgcgtaac aacgcaggga tggtagtcac
    11521 gctgatggac tggggtgcga ctttactttc agcccgtatt ccgctttccg atggcagcgt
    11581 ccgcgaggcg ctgctcggct gtgccagccc ggaatgctat caggatcagg ccgcgtttct
    11641 gggtgcctct attggtcgtt atgccaaccg catcgccaat agtcgttata cctttgacgg
    11701 tgaaaccgtg acgctttcgc caagtcaggg cgttaaccag ctgcacggcg gcccggaagg
    11761 gttcgacaaa cgtcgctggc agattgtgaa ccagaacgat cgtcaggtgc tgtttgccct
    11821 gagttcagat gatggcgatc agggcttccc gggtaatctc ggcgcgacgg tgcaatatcg
    11881 tctgaccgac gataaccgta tctccattac ttatcgcgcc acagttgata aaccttgccc
    11941 ggtgaatatg actaatcacg tctatttcaa tcttgacggc aagcagtctg acgtgcgcaa
    12001 tcacaagttg cagattctgg cggacgaata tctgccggtt gatgaaggcg gtattccgca
    12061 cgacggcctg aaatctgtcg ccggaacgtc ttttgatttc cgcagcgcca aaatcatcgc
    12121 cagtgagttt cttgccgacg acgatcagcg caaagtgaaa ggttacgatc acgcattctt
    12181 gttacaggcc aaaggcgatg gcaagaaagt ggcggcgcat gtctggtcag cagatgaaaa
    12241 attgcagctg aaggtctaca ccaccgctcc ggctctgcaa ttctactccg gcaactacct
    12301 cggcggcaca ccgtcgcggg gaaccgaacc ttacgccgac tggcaagggc tggcgttgga
    12361 aagcgagttt ctgccggaca gcccgaacca ccctgaatgg ccgcaaccgg actgtttcct
    12421 gcatcctggt gaagagtatt ccagcctgac ggaatatcag tttattgctg agtaatgtta
    12481 agccctcgtc atgagggctt tatctcatat tgttcaaatc accagcaaac accgacatat
    12541 ttgcaactca atattcacaa caaccttaca ctgcgccact attttcgcta tggttatgcg
    12601 taagcattgc tgttgcttcg tcacggcaat ataatgagaa ttattatcat taaaagatga
    12661 tttgaggagt aagtatatgg ctgtaactaa gctggttctg gttcgtcatg gcgaaagtca
    12721 gtggaacaaa gaaaaccgtt tcaccggttg gtacgacgtg gatctgtctg agaaaggcgt
    12781 aagcgaagca aaagcagcag gtaagctgct gaaagaggaa ggttacagct ttgactttgc
    12841 ttacacctct gtgctgaaac gcgctatcca taccctgtgg aatgtgctgg acgaactgga
    12901 tcaggcatgg ctgcccgttg agaaatcctg gaaactgaac gaacgtcact acggtgcgtt
    12961 gcagggtctg aacaaagcgg aaactgctga aaaatatggc gacgagcagg tgaaacagtg
    13021 gcgtcgtggt tttgcagtga ctccgccgga actgactaaa gatgatgagc gttatccggg
    13081 tcacgatccg cgttacgcga aactgagcga gaaagaactg ccgctgacgg aaagcctggc
    13141 gctgaccatt gaccgcgtga tcccttactg gaatgaaacc attctgccgc gtatgaagag
    13201 cggtgagcgc gtaatcatcg ctgcacacgg taactcgtta cgtgcgctgg tgaaatatct
    13261 cgataacatg agcgaagaag agattcttga gctgaatatc ccgaccggcg tgccgctggt
    13321 ttatgagttt gacgagaact tcaaaccgct gaaacgctat tatctgggta atgctgacga
    13381 gatcgcggcg aaagcagcag ccgttgcgaa ccagggtaaa gcgaagtaaa cgtcattcgt
    13441 ttaaaaatga gaaagccgac tgcaagtgag tcggcttttt tgtttgtcgt cgctaacaat
    13501 ggagcacatt gcctgatgcg acgctgcgcg tcttgtcagg gctacggtga ttcatcggat
    13561 acgccactct gacggcgcat ccgacaatta aactttaccc gcgacgcgct ttcactgcgt
    13621 tcgccagttg acgtaacaga gcatcggtat cttcccagcc aatgcaggca tcggtgatgc
    13681 tcttaccata ggccagcggc tccccgctct ccaggctctg attgccttcc accagatgac
    13741 tttccaccat cacaccgata atggcctttt cgccaccggc aatctgctgg caaacgtcag
    13801 cacaaacatc catctgcttt ttgaattgtt tggacgaatt agcatggctg aaatcgatca
    13861 tcacctgagc tggcagacct gctttgttca gcccttcttt cacttcagca acgtgcttcg
    13921 cgctgtagtt aggctcttta ccgccgcgca gaatgatatg gcaatcgccg ttaccgctgg
    13981 tattcacaat cgccgaatgc ccccatttag ttacggacag gaagcagtgc ggcgcaccgg
    14041 cggcattaat ggcatcgata gccactttaa tcgtaccgtc tgtgccattt ttaaaaccta
    14101 ccggacaaga aagaccagac gccagttcgc ggtgcacctg cgattccgtg gtacgtgcac
    14161 caattgcgcc ccagctcatc aggtcagcga gatactgagg agtgatcata tcgaggaatt
    14221 cacccgccgc tggcagaccg ctgtcgttaa tatcaagcag caatttacgg gctatacgca
    14281 gaccgtcgtt gatctggaag ctgttatcca tatgcggatc gttaatcagc cctttccagc
    14341 ccaccgtagt acgcggcttt tcaaaataga cgcgcattac gatttccagc tcatctttca
    14401 gctcttcacg cagcgccagc aagcgagtgg catactcttt agccgcgaca ggatcatgaa
    14461 ttgagcacgg gccaatcacc accaacaggc gatcatcatt acctttcagg atcttatgga
    14521 tcgcttttcg ggcatgggca accgtattcg cggcattttc agtagcgggg aatttttcca
    14581 gcaatgcgac aggaggaagt aactctttga tttctttgat gcgtaaatcg tcgttctgat
    14641 aattcatgtc tgttccagtg ttgccatact tatcttagtg aatgcaatac ttccaatcta
    14701 tatcttccgt cagaatgtgt aaacggggtt ttacactatg aacggattaa tcctggaatt
    14761 agcaagaaaa acgccagatt gtcgcgaaaa acgagatctc tcctacaatt tctaactgta
    14821 actcctttgt ttgttaatta tttcaagatt ctctgctgga tttctttgcc tggctgaaat
    14881 cttaaaccaa tgccttatat tcacctgcaa atgcactgtt ggaagaggtt atccgacata
    14941 acgaccataa caggagcatc ctatgaaaat gacaaaactg gccacactat ttctgactgc
    15001 cactctaagc cttgccagcg gtgccgcact ggctgccgat agcggagcgc aaaccaataa
    15061 cggccaggca aacgccgcag ctgatgcggg ccaggtagcc cctgacgccc gtgaaaatgt
    15121 cgcgccaaat aacgtcgaca ataacggggt aaataccggt tctggcggca ctatgctgca
    15181 tccggatggt tcttcaatga acaatgacgg catgaccaaa gatgaagagc acaagaacac
    15241 catgtgcaaa gatggtcgct gcccggatat caataaaaag gtacaaaccg gtgatggcat
    15301 caataatgat gtcgatacca aaaccgacgg taccacgcag taaccctgca aaatagccaa
    15361 taatgggaga gcggagtctc tcccttttaa tttttccctt ctaaaagcag tatgatgtca
    15421 taaagttaag tgagaataat taagggatga ctcgtcatgg cgcactcaca ctcacacacg
    15481 tcctcacacc tgccagaaga taataatgct cgtcgcttgt tgtatgcttt cggcgtgact
    15541 gccgggttta tgctggtaga agtcattggt ggttttcttt ccggttctct ggctttgctg
    15601 gccgatgcgg gtcatatgtt gaccgatact gccgccctgc ttttcgccct actcgccgtg
    15661 caattttcac gtcgtcctcc caccattcgc cacactttcg gttggctaag attgaccacg
    15721 ctcgcggcgt ttgtgaatgc catcgccctc gtggtaatta ccattttgat tgtctgggag
    15781 gcgatagaac gcttccgcac gccgcgtccg gtcgagggcg gcatgatgat ggcaattgcc
    15841 gtggctgggt tgctggcaaa tatactttct ttctggttac ttcatcacgg cagcgaagag
    15901 aaaaacctca acgtacgagc agcggcactg catgtgctcg gggacctgct gggttcggtt
    15961 ggggctatta ttgccgcgtt gattattatc tggaccggct ggacacctgc tgaccccatt
    16021 ctctcgatac tggtgtcgct tctggttctg cgtagcgcgt ggcgactgtt gaaagatagc
    16081 gtgaatgaat tacttgaggg tgcaccggta tcgctggata tcgctgaact gaagcgtcgt
    16141 atgtgccggg aaatcccgga agtccgcaat gtgcaccatg tgcatgtatg gatggtaggc
    16201 gagaagccgg tgatgacgtt gcatgtgcag gtgatcccgc cacacgatca cgatgccttg
    16261 ttggatcaga tccaacacta cctgatggat cactatcaga ttgagcacgc caccattcag
    16321 atggaatatc aaccttgtca tgggccagac tgccacctga atgaaggggt gtctggccat
    16381 tcacatcatc accattaatg ggacagcgcg cgtgagcctc tttcgcgtgc gctattgatc
    16441 cacatccggc tgccgttgag cgcaataaag gtcaggatga tgtactccag agacatggcg
    16501 taaacgccct gaagtgcaaa aataacgacg ctaatcacgt taataatcac ccacaacagc
    16561 cagttttcca catacttacg agtcatcaga atcattgcca cgatagataa caccatcatg
    16621 caagaatccc agaacgggaa agcgtccggt tgtagttcag gcatcgcaac ctgtaatcct
    16681 aatgcttgca tgatgatgac tgccacgcgg gtcaaaaatg caaacaccgg attgataaat
    16741 accgtcatca gaccaatcga aacaacgcaa accgccaacc agctgagtgc cttcggcaat
    16801 ggcaaccagc gaatttttaa ctccgcctcg ttctgactgg tttgtcgcga ccacgcatac
    16861 cagccgtaaa tattcgcggc aaagaaaaac acctgtaata gcaggctggc atacagctga
    16921 atctgaaaga aaataatgcc aaacaaggtg acgttgatca ggccaaagaa gtagttgctg
    16981 atcttctcca gactggcaag gccaatacac agcaacccgg cgattgtgcc taccgcttcg
    17041 atccatgaga gatcataacc gcctgctcct attggtatat gtaccaggat attctgcaca
    17101 ctaaaaaaat ccatctttcc cccaaagcgt aacgttacgc cttattattc gttatccacg
    17161 tagtgtagcc gcaaaatcca gcatacgatt gagcggcacc agcgccctct ctcgcagcct
    17221 ttcatcaaca tgaacctcgt aattgcttcc ttcctgttct aatgcctcag cgatggcctg
    17281 gaggccattc atggccatcc acggacaatg cgcgcagctg cggcaggttg cgccctcacc
    17341 cgcggttggt gcttccagta actctttatc tggcaccgcc tgctgcattt tgtagaaaat
    17401 accccgatcg gttgccacaa taagcctctg atgtggcaat gttttcgcag cagcgatcag
    17461 ttgactggtg gaaccgaccg catccgccat atcgacaata gcttgtggtg attctggatg
    17521 caccagtatg gcagcatccg ggtattcttc ttgcaagcgg gttaacgcct gagtcttaaa
    17581 ctcatcatgc acaatgcagg caccctgcca gcataaaatg tctgcgcccg tctttttttg
    17641 cacgtaacgc cccagatgtt tgtcgggtgc ccagatgatt ttttcaccca aactatccag
    17701 atgatcaata agttcgacgg caatgcttga agttaccacc caatctgccc gcgcttttac
    17761 cgcagcagaa gtgttggcat agacgacgac agtacgatcg ggatgggcat cgcaaaatgc
    17821 gttaaattct tcaacagggc agccgagatc cagtgaacac tcagcctgaa gtgtcggcat
    17881 cagaattgtt ttttccggac tgagaatttt ggcggtttct cccataaatc tcaccccagc
    17941 gactaacaac gtagaagcgg gatgctttgc accgaagcgc gccatttcca gagaatcaga
    18001 aatacagcca ccggtttctt ctgccagttg ttgaatttcg ggatcggtat agtagtgggc
    18061 aaccatcacc gcattacgtt cttttagcag acgttttatc ttctcgcggt aatacgcttt
    18121 ttcatcaatg cttaacggcg tcggcttcgg ggggaaagga taaatcgccg tgtctggatc
    18181 aaacatcacg ctcatcttac catctcgttt tacaggctta acgttaaaac cgacattagc
    18241 gtgatattcg ctgttatcgg tgtaatttgt tttatatgct aaacaagata gtcgaatggt
    18301 ggggaaaagt cactcgaatt acgtatgaga tgccgaatgc cacactttgc gtcttattcc
    18361 tccgacgtaa agttgtttac gcagcaagcg cggagtatgt catcagggtc aagaattgaa
    18421 attgagagat gcctgaatag caaaaaagcg cctttagggc gcttttttac attggtgggt
    18481 cgtgcaggat tcgaacctgc gaccaattga ttaaaagtca actgctctac caactgagct
    18541 aacgacccat acgggtgtta cctgaaagat ttctactcgg aaccaatctt taaaattggt
    18601 gggtcgtgca ggatgactcg cttcgcttgc cctacgggcc gttgctgacg caacgttatc
    18661 cttcacgctt aacatctgag tttgatgtta aaattggtgg gtcgtgcagg attcgaacct
    18721 gcgaccaatt gattaaaagt caactgctct accaactgag ctaacgaccc gagtggtggg
    18781 tgatgacggg atcgaaccgc cgaccccctc cttgtaaggg aggtgctctc ccagctgagc
    18841 taatcacccg atactgcgct ggatactaca aactacttcc accgaaccca ccgtaattag
    18901 tggtgggtcg tgcaggattc gaacctgcga ccaattgatt aaaagtcaac tgctctacca
    18961 actgagctaa cgacccggtg gtgggtgatg acgggatcga accgccgacc ccctccttgt
    19021 aagggaggtg ctctcccagc tgagctaatc acccgatatt acgctggata ctacaactaa
    19081 ctaggttagt ggtgggtcgt gcaggattcg aacctgcgac caattgatta aaagtcaact
    19141 gctctaccaa ctgagctaac gacccacttt ttcgttgctt tcggtttgtt tgatatcccg
    19201 tggcaacggc ggcatatatt actgatttca gacttgagcg caacaaaaat ttcgatgtag
    19261 atcactcaac tgcttatgat tcgcacgaca cgaccagaaa taatgcgact tctggtcgtg
    19321 tgttatgcat tacatcgcgt tcagacgttt ttgtgcctgt ttagcgccat cagtaccagg
    19381 gtatttactg ataacctgct ggtatacggc tttcgctttt gcggtgtcac ctttgtcctg
    19441 catgatgacg ccgactttaa acatcgcatc tgcagccttt ggtgacttcg gatagttttt
    19501 cactaccgaa gcaaaatagt acgccgcatc atctttttta cccttgttgt agtttaactg
    19561 acccagccaa taattggcgt ttggcaggta agttgaatca gggtaatttt tgatgaaatt
    19621 ctgaaatgcc accattgcgt catcctggcg ggatttatcc tgcaccagcg caatagctgc
    19681 attgtaatcc gtgtttgcat caccgctttt caccggcgcg ccagcattcg cagtaccagc
    19741 atcagctgtc ggcgtcgttg atgccgccgc accgctttga tcaccgctgg ttgattgcgc
    19801 cgctgcacca ccgctgctga ggctgtcgat ctgcaacagg atctgctttt gccgctccac
    19861 gacctgattc agttgatact gattttcctg aatctgacca cgcagggaat caatatcgga
    19921 ttgattatca gagagttgtt gctggagttg ggttaaaagc tggctgtgag cattagaaat
    19981 acgctcaagt tgaatgacgc ggtcttcgac cgagcctgag ccgacactac tgattggtgc
    20041 ctgagcaaaa gcggcccagg gggccgctat accaaccagt aacgacagac tcaatagttg
    20101 atgtctgaag ttactgctca tgcaattctc ttagtaaacc agtaccgcac gacggttttt
    20161 ggagtatgcc gcttcgtcat gacccagtac tgcaggtttt tctttaccgt aagaaacgat
    20221 ggagatctgg tctgcagaaa cgcctttacc ctgcaggtac atcttaacgg cgttcgcacg
    20281 acgttcaccc agggagatgt tgtattccgg agtaccacgt tcgtccgcgt gaccttctac
    20341 ggtgactttg taagacgggt tgctacgcag gaagtttgca tgtgcatcca gcatttgagc
    20401 gaagtcagaa cggatatcgt acttgtccag atcgaagtaa acgatgttgt tctgctgcag
    20461 ctgttgcatt tgcagacgag cctgctcttc ggaagacatg ttgccgttgc cgccgttcgc
    20521 atccatacca gtgccggcac ccagcatgcc ttcgctgccg tcattgctgg cgttcttgtt
    20581 ggaagaacat gccgcaattg ccataacagg cagagcaatc atcagccctt tcagcacttt
    20641 gttcagttgc atttcaatga ttcctttact attcaattaa ttattatcac agatacggcg
    20701 accaggcagg gaatttgacc tgtccatcag ttgccggaag acgcgctttg aaacgcccat
    20761 ctgtagaaac caaattcagc acggatccca tcccctgaga agagctgtag attaccatag
    20821 tgccgttagg tgccagactt ggcgtttcat ccaggaacgt ggacgacaga acttgtacgc
    20881 ctcccgttgc cagatcttgt ttggcaatgt gctgctgccc accattggag ctgaccatta
    20941 ccataaattt accgtcgctg ctgacatccg catcctggtt ctgcgaacct tcccaggtaa
    21001 tacgttgtgg cgcaccgccg ttgatattca ctttataaac ctgtggacga ccggcctggt
    21061 cagaagtaaa tgccaggttc tggctgtccg ggaaccaggt cggttcggta ttgttactgc
    21121 gaccatcagt cacctggcgg atctgaccag aagccaaatc cattacgtac aggttcaggc
    21181 taccggtttt cgacaaggcg aatgccagtt tgctgccgtc tggcgagaat gcaggcgcac
    21241 cgttgtgacg cgggaatgaa gccacctgac gtacagcgcc gtttgccagc gtttgtataa
    21301 ccagcgcgga acgaccgctt tcgaaggtca cataagccag tttagaaccg tctggtgacc
    21361 acgccgggga catcagcggc tgtggtgaac ggtgaacgac aaactggttg taaccgtcat
    21421 agtcagatac gcgcagttca tacgggaact gaccgccgtt ggtctgaaca acgtaggcaa
    21481 tacgggtacg gaacgcacct ttaataccgg tcagtttttc aaacacttca tcactggcgg
    21541 tatgaccagc ataacgcagc cactgcttgt tcactttgta cgagttctga gcaagtacag
    21601 tacccggtgc gccgccagtg tcaacaagtt gataagcaac attgtaagaa ccatccggat
    21661 tcggagtgac ctgaccgaca actacagcgt caatacccag cgcggaccat gcagctggtt
    21721 gtacttcctg cgcactaccc ggctgctgtg gcagacgagc gcgatctaac ggattaaatt
    21781 taccgctgtt acgcaagtcg gcagcaacga tgccgccaat atcttcaggt gccgcaccag
    21841 ggcccgccca ctggaaagga acaactccaa taggacgacc ggaatctaca ccgctgtcga
    21901 tcacaatgcg gacttcagca tgcagaactg atgcccacag tatgagaaaa ccaaatgcta
    21961 ctcgtaatgc ctgcttcatc atatctccct tatctggacc gatggcccac gataatttag
    22021 cagaatgtta acaaactaaa atacacaaaa ctaccagaac cccgtggcaa ccggtgcctg
    22081 atgttgaccg tcggaacagt caacatcgcg attacggttt gaagtccaat ggcgcgtttt
    22141 tgaacacttc atatactgcc tggcttggtg gtttcgggat cttcgcaagt ttagctgctg
    22201 ccaacgcagc ctgacaaagt gcgggatcgc caccttcagg tttgatatcc agtaacatac
    22261 catcgggtgc cagttttatg cgcagcgtac aggttttgcc tgcataggac gatgcgtcat
    22321 agaacttact ttcgatagca gatttaatct gcccggcata gttattgata tcggcccctg
    22381 atgcgccatt gtttttagta ttaccactcc cggcaggcga agcattgttc cctttcgccc
    22441 ctccccccgt tttcggtgca ttcttaccag agcttagctc accgaaaata tcatctgcct
    22501 ccgcggcagc ttttgctgca gcggcttttt cagctgctgc ttttgcagct gccgcttttt
    22561 tgtctgcggc ggctttttct gccgctgctt tcttatcagc tgctgccttt tcagcagccg
    22621 ctttttctgc tgcttttttg tcggctgcag ctttctctgc tgctgccttt tcagcagccg
    22681 ctttttctgc tgcttttttg tcggctgcag ctttctctgc tgcttttttg tcggctgcag
    22741 ctttctctgc tgccgctttc tttcttgctt cagctgcagc tgcttctgcc gcttccgctt
    22801 tcttcttcag cgccgcagct gctgcctcgg cttttttctg cgcttcggct gcggctttgg
    22861 cggcttctgc ttctgctttt ttcttcgcgt ctgcagccgc tttcttcgct gcttcttccg
    22921 cagctttatc atccgcttcg gccttcgctt tagcatctgc cgccgctttc gctgccgcct
    22981 cttccgcttg cttctgcttt aactcggcct gttttgcggc ttcttcagcc tgctttttct
    23041 gttcctgagc agctaaccgc tctttctcaa gttgcttcag gcgttcctgt tcagccgctt
    23101 gtttctcacg cagttcttca gcagcctgct gttccttcat cttgcgctgc tcatcagaac
    23161 gcttcgcgct tgattcctgg ctttgcatgc gtttgtactg ctcaactacc gcacctgaat
    23221 caaccatgac agcgtcgatg gacgaaccac cgccgcctcc agctgaagct tctatattct
    23281 catcgaacga actccagatc agcgccgcaa ataagatgac atgcagcact gctgaaataa
    23341 ttatcgcccg cttgagcttg tcgttttgtt cggttgcctt tgacactctc ggtttccaaa
    23401 aactgttcgc ctgttacccg ctctctttca agcaagggaa acgcagatgt ttagataggc
    23461 tgcgtcatta aaccaaccga tttcacaccc gcactatgta acaagttcag tgctttaatt
    23521 atttcatcgt aaggcacatc ttttgcgcca ccgatcagaa agaccgtttt cgggttggcc
    23581 ttgaaacggc tggacacttc cgccaccacc tgctctggtg gtaaacgctc caggcgatct
    23641 ttctcaacca ccacggtgta ctgaccaata ccagacactt caacaatcac tggcggatta
    23701 tcgttactgc tcaccgcctg tgattcagta gcgtctggca aatcgacctc cacgctctgg
    23761 gtgatgatgg gcgctgtcgc cataaagatc agcaacagca ccagcagtac gtccagcaac
    23821 ggtacaatgt tgatttcgga cttgagatcg cgacgacctc gtccacgcgc tctggccatg
    23881 gcttacccct tgttgctctc gctaacggta aacgcctggc ggtgcagaat cgcggtaaac
    23941 tcttccataa agttgtcgta attcagttcc agtttgttta cacgttggtt gagacggtta
    24001 taagccatta ccgccggaat agcggcgaac agaccaattg ccgtcgcaat caacgcttct
    24061 gcgataccgg gcgcaaccat ttgcagtgtt gcttgtttta ccgccccgag ggcgataaag
    24121 gcgtgcatga tcccccagac cgtaccaaac agaccaatat acgggctgat ggagccaacc
    24181 gtaccgagga acggaatgtg cgtttccaga ttttcaagtt cgcggttcat ggaaatacgc
    24241 atcgcacgcg acgccccttc cactacggct tccggcgcat ggctattggc acgatgcagg
    24301 cgcacaaatt ctttgaaccc gctgtaaaag atttgttccg aaccagtcag attatcccgt
    24361 ttcccctggc tctcttgata gaggcgagag agttcgattc cagaccagaa tttatcttca
    24421 aacgcttcgg cttcgcgcgc cgctgcgtta agaatacggg tccgctggat aataatggcc
    24481 caggatgcga ttgaaaaacc aatcaaaatc aacatgataa gtttaaccag aaggctagcc
    24541 ttcaggaaca aatcaaggat attcatgtca gtcactgctt aaactccgcg acaatagact
    24601 tgggaagcgc acgaggcttc attttgagtg ggtcaacgca aacaaccaga acctctgctt
    24661 cattcagcaa agtattctcg gcgttgacaa tacgttgcgt gaaaaccaaa gaggtgccac
    24721 gcattgatgt tatttcagtc tgtatttcga gcatatcgtc gagccgcgca ggtgcgtaat
    24781 attccaccgt cattttacgt accacaaagg caacgcgttc agccatcagc gcctgttgac
    24841 tgaagtgatg atgacgcagc atctctgtgc gtgctctttc ataaaaagcg acgtaactgg
    24901 cgtggtacac cacaccaccg gcatcggtat cttcatagta gacgcgaacc ggccatcgaa
    24961 acagcgttgt attcacttta catcccggta atgcaacaaa agttagagct tttaaacgct
    25021 gctactatac gcgcgcgatt gaggtttggg aatgagggca agttaaggga gcgtaaaaat
    25081 ttatgggcct ggcacggccc ataatttttt aaagagaatt ctgatttaaa agaagaagaa
    25141 aatcagcccg acaatgagaa caatatcggc aagcaatggg caaaaaatcc cttgccaaag
    25201 aaccttctgc ggacgaaagc ccacgccgtg aatcacacca gcacagacgg cccacatcag
    25261 caataaacca tgccagattt ccagttcact ggtcttcgcg gcgaaacgag atgggtccca
    25321 aaacatacat cctgctaaca gaagcgccat cacgaaggaa agcgcccgca gggggcgctt
    25381 gtccattacc gcatacaagg ttgcgataat cttactcatc agatgtcttc ttgaccggct
    25441 ttgcctgatt cgacgtgctc aagcgccagc gcggtgatta ccccaaacga acaggcaaga
    25501 agcgttccca gaatccatgc gaaataccac atttttagct ccttacttag tacagagagt
    25561 gggtgttacg ttcaatatct tctttggtga tacgaccgaa cattttccag taacaccagg
    25621 cggtgtagag cagaatgatc ggtaccagaa ccaccgcaac ccaggtcatg acgttaagtg
    25681 tcagctggct ggaagttgca tcccacattg tcagacttgc gttcatcatg gtgctggacg
    25741 gcatcacaaa cgggaacatt gcgataccgg cagtcaggat gatgcaggcc agcgtcaggg
    25801 aggagaacac aaacgcccac gcggctttat ccatacgtgc agtcaggatg gtcagcagcg
    25861 gcagaaccac acccagtgcc ggaatagccc acagaattgg cgtgttgttg aagttaacca
    25921 gccatgcgcc agcttcacga accacttctt tattcagcgg gttagaggct gcgtaatggt
    25981 ccattgtcga tttcacgaca taaccatcga taccgtacat cacccatacg ccagccagtg
    26041 cgaaacagac cagtgtcacc agtgccgcca cctgagccgt tgcacgggta cgcaggtgca
    26101 gttcgcccac ggtacgcatt tgcagatagg ttgcgccctg agtaatgatc atccctacgc
    26161 tcaccacgcc tgccagcagg ccgaacgggt taagcagctg gaagaagtta ccggtgtagt
    26221 acagacgcag atattcatca acgttgaacg gtacgccctg caacaggtta ccgaacgcta
    26281 caccaatcac caacggcgga acgaagctac caatgaagat gccccagtcc cacatgttac
    26341 gccagcgggt ttcttcaatc ttggagcggt agtcaaaacc gaccggacgg aagaacaaag
    26401 acgccagcac gaggatcatc gccacataga agccggagaa cgcagcggca tagaccatcg
    26461 gccaggcagc aaacagtgcg ccgcctgcgg tgatcagcca aacctggtta ccgtcccagt
    26521 gtggtgcgat ggagttaatc ataattcgac gctcggtgtc gttacgaccg aggaaacggg
    26581 tgagcatgcc cacccccatg tcgaaaccat cagtgactgc aaaaccaatc agcagaacgc
    26641 caaccagcag ccaccagata aaacgcaata cttcataatc gatcatttga cgactcctgt
    26701 cttagcgtgc cggctgagta gtcgtggaag actgctcaaa gtgatagcga ccggttttca
    26761 ggctgcttgg gccgaggcgt gcaaacttga acattaagaa caattctgcc accaggaaca
    26821 gggtatacag gccgcaaatc agcaccattg agaagatgag atcgcctgcg gtcagtgacg
    26881 agttcgccac agctgtcggc agcacttcac cgatagccca cggttggcgg ccatattcag
    26941 ccacgaacca gcccgcttct acagcaatcc acggcagcgg aataccgtac agcgcggcgc
    27001 gcaggagcca ttttttctcg ccaatgcggt tgcggatgac actccagaaa gagagcgcga
    27061 tgattgccag cagcaggaag ccacacgcca ccatgatacg gaacgcgaag tacagcggcg
    27121 ctacacgcgg aatggagtct ttggttgcct gttgaatctg cgcttcagtc gcatcagcca
    27181 cgtttggcgt atagcgtttc agcagcagac cgtaaccgag gtccttcttc atgctattga
    27241 actggtcacg aaccgcctgg tcggtagaac cagaacgcag ttgttcgagc agagagtacg
    27301 ccttcatccc gttacgaatg cgttcttcat gctgcaccat cagttctttc aggccgataa
    27361 ctggagtatc cacggaacgc gttgcaatta tgcccagcgc gtaagggatc tggatcgcaa
    27421 atttgttcgt ctcctcttcc tgatcaggaa tgccgaacag agtaaaggca gcaggcgcag
    27481 gttgcgtttc ccactcagct tcaatagcag ccagtttggt tttctgcacg tcgcccattt
    27541 cgtagccgga ttcatcaccc agaacaataa cagacagaac ggcagccata ccgaagctgg
    27601 cagcgatagc aaaggagcgt ttagcgaagg cgaagtcacg acctttcagc atataccatg
    27661 cgctgatacc gaggatgaac atcgcgccag tcacataacc agacgctaca gtgtgaacga
    27721 atttcacctg agcaaccggg ttaagcacca gctcggagaa gctcaccatc tccatacgca
    27781 tagtttcaaa gttgaaatcg gacgcgattg ggttttgcat ccagccgttc gcaaccagaa
    27841 tccacagtgc ggacaggttt gaaccgagcg ccaccagcca ggtgacacac atatgctgaa
    27901 ctttacccag acgatcccaa ccgaagaaga acagacctac aaaggtggat tcgaggaaga
    27961 aggccatcag accttcgatt gccagcggcg caccgaagat atcccctaca tagtgggaat
    28021 agtaagacca gttagtcccg aactggaact ccatggtcag accggtagcc acacccagag
    28081 cgaagttgat accaaacaac ttgccccaga acttggtcat atctttataa atctgtttgc
    28141 cggaaaggac gtagaccgtt tccataatgg ccagcaggaa cgccataccg agcgtcagtg
    28201 gcacaaaaag gaagtggtac atcgcggtca aggcaaactg taagcgcgac agttcgacta
    28261 tatctaacat catgactcct tgctcatcgc atgaagactc cgagagtgaa ccccgttaga
    28321 aagggtcaca cgcatgcccc aatacaaaat tatttgctcg cctctatcgt tgccatttca
    28381 tcaaaagagg aaaagacaat gataaaaaag ttacaaatat gttaatgaat aaaccaaatt
    28441 gatcccgtaa ttatattacg ccgcaaaatc cttacaataa acaggttttt attgagcaaa
    28501 tttgcatttt tcgacagtga tcaatttata gcgaaattgt cacgatctca ccaatttgat
    28561 ctacgacaat ttatcgtgtt ttgcagattt ttttcaagaa ttaaaaattg atttatatca
    28621 aaaccaacca aatatgttaa ttatgtataa accatagatc attggtgatt ttattgttaa
    28681 ttta
//
LOCUS       DBFIFI010000042        28437 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.042, whole genome shotgun sequence.
ACCESSION   DBFIFI010000042 DBFIFI010000000
VERSION     DBFIFI010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.042"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..536)
                     /locus_tag="N7B39_001743"
     CDS             complement(<1..536)
                     /locus_tag="N7B39_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HGU2421739.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKAL"
     gene            complement(607..2721)
                     /gene="fusA"
                     /locus_tag="N7B39_001744"
     CDS             complement(607..2721)
                     /gene="fusA"
                     /locus_tag="N7B39_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HGU2421740.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2818..3288)
                     /gene="rpsG"
                     /locus_tag="N7B39_001745"
     CDS             complement(2818..3288)
                     /gene="rpsG"
                     /locus_tag="N7B39_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HGU2421741.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3385..3759)
                     /gene="rpsL"
                     /locus_tag="N7B39_001746"
     CDS             complement(3385..3759)
                     /gene="rpsL"
                     /locus_tag="N7B39_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HGU2421742.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3885..4172)
                     /gene="tusB"
                     /locus_tag="N7B39_001747"
     CDS             complement(3885..4172)
                     /gene="tusB"
                     /locus_tag="N7B39_001747"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HGU2421743.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSGQMAW"
     gene            complement(4180..4539)
                     /gene="tusC"
                     /locus_tag="N7B39_001748"
     CDS             complement(4180..4539)
                     /gene="tusC"
                     /locus_tag="N7B39_001748"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HGU2421744.1"
                     /translation="MKRIAFVFSTVPHGTAAGREGLDALLATSALTDELAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLDLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4539..4925)
                     /gene="tusD"
                     /locus_tag="N7B39_001749"
     CDS             complement(4539..4925)
                     /gene="tusD"
                     /locus_tag="N7B39_001749"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HGU2421745.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANHLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDEMEAGRLGLASSN
                     LQQGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4925..5647)
                     /gene="yheO"
                     /locus_tag="N7B39_001750"
     CDS             complement(4925..5647)
                     /gene="yheO"
                     /locus_tag="N7B39_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU2421746.1"
                     /translation="MSRSLLTNETSELDLLDQRPFEQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5814..6626)
                     /gene="fkpA"
                     /locus_tag="N7B39_001751"
     CDS             complement(5814..6626)
                     /gene="fkpA"
                     /locus_tag="N7B39_001751"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HGU2421747.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6847..7065
                     /gene="slyX"
                     /locus_tag="N7B39_001752"
     CDS             6847..7065
                     /gene="slyX"
                     /locus_tag="N7B39_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HGU2421748.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(7114..7704)
                     /gene="slyD"
                     /locus_tag="N7B39_001753"
     CDS             complement(7114..7704)
                     /gene="slyD"
                     /locus_tag="N7B39_001753"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HGU2421749.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7799..7999)
                     /gene="yheV"
                     /locus_tag="N7B39_001754"
     CDS             complement(7799..7999)
                     /gene="yheV"
                     /locus_tag="N7B39_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HGU2421750.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(8036..9814)
                     /gene="kefB"
                     /locus_tag="N7B39_001755"
     CDS             complement(8036..9814)
                     /gene="kefB"
                     /locus_tag="N7B39_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HGU2421751.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERR"
     gene            complement(9814..10365)
                     /gene="kefG"
                     /locus_tag="N7B39_001756"
     CDS             complement(9814..10365)
                     /gene="kefG"
                     /locus_tag="N7B39_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HGU2421752.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10496..12409
                     /gene="yheS"
                     /locus_tag="N7B39_001757"
     CDS             10496..12409
                     /gene="yheS"
                     /locus_tag="N7B39_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU2421753.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12409..13431
                     /gene="yheT"
                     /locus_tag="N7B39_001758"
     CDS             12409..13431
                     /gene="yheT"
                     /locus_tag="N7B39_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HGU2421754.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRKVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQAQHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13425..13643
                     /gene="yheU"
                     /locus_tag="N7B39_001759"
     CDS             13425..13643
                     /gene="yheU"
                     /locus_tag="N7B39_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HGU2421755.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13697..14566
                     /gene="prkB"
                     /locus_tag="N7B39_001760"
     CDS             13697..14566
                     /gene="prkB"
                     /locus_tag="N7B39_001760"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HGU2421756.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAIELIML
                     PLVQRLMEGKKIE"
     gene            complement(14621..15025)
                     /gene="yhfA"
                     /locus_tag="N7B39_001761"
     CDS             complement(14621..15025)
                     /gene="yhfA"
                     /locus_tag="N7B39_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HGU2421757.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15327..15959
                     /gene="crp"
                     /locus_tag="N7B39_001762"
     CDS             15327..15959
                     /gene="crp"
                     /locus_tag="N7B39_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HGU2421758.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            16010..18100
                     /gene="yhfK"
                     /locus_tag="N7B39_001763"
     CDS             16010..18100
                     /gene="yhfK"
                     /locus_tag="N7B39_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HGU2421759.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(18167..19387)
                     /gene="argD"
                     /locus_tag="N7B39_001764"
                     /gene_synonym="dapC"
     CDS             complement(18167..19387)
                     /gene="argD"
                     /locus_tag="N7B39_001764"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HGU2421760.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPSDIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGQARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19473..20036)
                     /gene="pabA"
                     /locus_tag="N7B39_001765"
     CDS             complement(19473..20036)
                     /gene="pabA"
                     /locus_tag="N7B39_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HGU2421761.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKVVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPDSLPACFEVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(20068..20670)
                     /gene="fic"
                     /locus_tag="N7B39_001766"
     CDS             complement(20068..20670)
                     /gene="fic"
                     /locus_tag="N7B39_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HGU2421762.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKTKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20660..20827)
                     /gene="yhfG"
                     /locus_tag="N7B39_001767"
     CDS             complement(20660..20827)
                     /gene="yhfG"
                     /locus_tag="N7B39_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HGU2421763.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRSHYER"
     gene            complement(20932..21504)
                     /gene="ppiA"
                     /locus_tag="N7B39_001768"
                     /gene_synonym="rotA"
     CDS             complement(20932..21504)
                     /gene="ppiA"
                     /locus_tag="N7B39_001768"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HGU2421764.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21775..22956
                     /gene="tsgA"
                     /locus_tag="N7B39_001769"
     CDS             21775..22956
                     /gene="tsgA"
                     /locus_tag="N7B39_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HGU2421765.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVAKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            23218..25761
                     /gene="nirB"
                     /locus_tag="N7B39_001770"
     CDS             23218..25761
                     /gene="nirB"
                     /locus_tag="N7B39_001770"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HGU2421766.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25758..26084
                     /gene="nirD"
                     /locus_tag="N7B39_001771"
     CDS             25758..26084
                     /gene="nirD"
                     /locus_tag="N7B39_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HGU2421767.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            26210..27016
                     /gene="nirC"
                     /locus_tag="N7B39_001772"
     CDS             26210..27016
                     /gene="nirC"
                     /locus_tag="N7B39_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HGU2421768.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGTISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            27035..28408
                     /gene="cysG"
                     /locus_tag="N7B39_001773"
     CDS             27035..28408
                     /gene="cysG"
                     /locus_tag="N7B39_001773"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HGU2421769.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         6858 a         7651 c         7211 g         6717 t
ORIGIN      
        1 agcgctttca gagcagaacc acgaacgatc ggagtgtcgt cgcccgggaa gtcgtactga
       61 gacagaagtt cacgaacttc catttcaacc agttccagca gctcttcgtc atcaaccatg
      121 tcgcatttgt tcaggaacac gatgatgtac ggaacgccta cctgacgacc cagcaggatg
      181 tgctcacgag tctgcggcat cgggccgtca gtcgcagcaa ctaccaggat cgcgccgtcc
      241 atctgagcag caccggtgat catgttttta acatagtcgg cgtgccccgg gcagtctacg
      301 tgtgcgtagt gacgggtcgg ggtgtcatat tcaacgtgag aagtgttgat ggtgatacca
      361 cgagcttttt cttccggcgc gttatcgatc tggtcgaatg cacgagcagc accgccgtag
      421 gttttagcca gtacggtggt gattgcagcg gtcagagtag ttttaccgtg gtcaacgtgg
      481 ccgatagtac caacgttaac gtgcggtttt gtacgttcaa atttttcttt agacacggct
      541 atattcctta ctatagtgct ctccccttca ggagagagca cgggactttg gtattaaccc
      601 ttaggcttat ttaccacggg cttcaattac ggcctgagca acgttactcg gcgcttcatc
      661 atacttcagg aattccatag tgtatgatgc acgacctttg gtcagagaac gcagctgagt
      721 tgcgtatccg aacatttcag acagcggtac ttcagcgtgg atcttaacgc cagtaacttc
      781 agattcctga cctttgagca taccacgacg acggctcaag tcaccgataa cgtcaccggt
      841 gttctcttcc ggagtttcta cttcaacctt catgatcggc tcaagcagaa ctggtttcgc
      901 tttcttaaag ccttctttaa aggcgataga agcagccagt ttaaacgcca gttcagagga
      961 gtcaacgtca tggtaagaac cgaagtgcag acgaataccc atgtctacta ccgggtagcc
     1021 tgccagcgga cctgctttca gctgttcctg gataccttta tcaacggccg ggatgtattc
     1081 gccagggatt acaccacctt taatgtcgtt gatgaactcg tagcctttcg ggtttgaacc
     1141 cggctccagc gggtacatgt cgataacaac atgaccatac tgaccacgac caccagactg
     1201 tttcgcgtgt ttaccttcaa catcggtaac tttctggcgg atagtttcac ggtaagcaac
     1261 ctgcggttta cctacgttcg cttcaacgtt gaattcacgc ttcatacggt caacgatgat
     1321 gtcgaggtgc agttcgccca tacccgcaat gatggtctgg ttagattctt cgtcagtcca
     1381 tacacggaaa gacgggtctt ctttagccag acggcccaga gccagaccca ttttttcctg
     1441 gtcagctttg gttttcggtt caactgcgat ggagattacc ggctcaggga attccatacg
     1501 ttccagaatg atcggcgcat ccgggtcaca cagggtgtca ccagtggtta cgtctttcag
     1561 accgatagca gcagcgatgt cgcctgcgcg aacttctttg atctcttcac gtttgttagc
     1621 gtgcatctga acgatacgac cgaaacgctc acgtgcagct ttcacggagt tcagtacggt
     1681 atcaccagag ttaaccacac cggagtaaac acggaagaag gtcaggttac caacaaacgg
     1741 gtcggtagcg attttgaacg ccagtgcaga gaacggctcg tcatcacttg cgtgacgttc
     1801 agccggagtg tctttaccgt cgtccaggat accgttgatc gcaggtacgt caaccgggga
     1861 tggcaggtaa tcaattaccg catccagcat cgcctgaaca cctttgttct tgaacgcaga
     1921 accacaggtt accaggatga tttcgttgtt cagaacgcgc tgacgcagag cacctttgat
     1981 ttctgcttca gtcagttctt caccacccag gtatttttcc atcagctctt cagaagcttc
     2041 agctgcggat tcgatcaggt tctggtgcca ttcgttagcc agttcaacca tgtctgccgg
     2101 gatatcttcg tattcgaagg ttacgccctg gtcagcgtcg ttccagttga tagctttcat
     2161 tttcaccagg tcaacaacac cggtgaaatg ttcttcagca ccaatcgcca gctgcagcgg
     2221 aaccgggttc gcgcccagac gggttttgat ctggttaaca actttcagga agttcgcacc
     2281 catacggtcc attttgttaa cgaacgcaat gcgcggaact ttatatttgt ttgcctgacg
     2341 ccatacggtt tcagactgcg gctgaacacc accaactgcg cagtaaacca ttaccgcacc
     2401 atcgagcaca cgcatggaac gttctacttc gattgtgaag tcaacgtgcc ccggggtgtc
     2461 gatgatgttg atgcgatgcg gctcatactg cttagccata ccagaccaga atgcagtagt
     2521 cgcagcggaa gtgatggtaa taccacgttc ctgctcctgc tccatccagt ccatggttgc
     2581 agcgccgtca tgaacttcac cgattttatg gtttacaccg gtgtagaaca gaatacgttc
     2641 ggtagtagtg gttttaccgg cgtcgatgtg cgcactgata ccgatgttac ggtagcgtgc
     2701 gatgggtgtt gtacgagcca tttgtttcct cgtttatctt ttaggcgttc aatttaagta
     2761 gcccaaagcg ggctgcttac tggaagcgcc cgcctggtga ctaaaactcc gaagggatta
     2821 ccaacggtag tgtgcgaacg ccttgttggc ttcggccata cggtgaacgt cttcacgttt
     2881 cttaactgca gtacctttgt tttctgcagc atcagaaagt tcgttcgcca ggcgcagagc
     2941 catggattta tcaccgcgtt tacgagcagc ttcaacgatc caacgcattg ccagagcatt
     3001 acgacgaacc ggacggactt caactggtac ctgataagta gaaccaccaa cgcggcgaga
     3061 cttaacttct acagtcgggc gcacgttttc gagagctact tcgaatgctt ccagttcaga
     3121 tttaccagag cgctgagcca gggtctccag cgcgctgtat acgatagatt cagcagtaga
     3181 ttttttacca tctaccatca ggatatttac aaatttagcc agcagttctg atccgaactt
     3241 cggatccggc agaattttac gctgaccaat gacgcgacga cgtggcatgg aaatactccg
     3301 ttgttaattc aggattgtcc aaaactctac gagtttagtt tgacatttaa gttaaaacgt
     3361 ttggccttac ttaacggaga accattaagc cttaggacgc ttcacgccat acttggaacg
     3421 agcctgctta cggtctttaa cgccggagca gtcaagcgca ccacgtacgg tgtggtaacg
     3481 aacacccggg aggtctttaa cacgaccgcc acggatcagg atcacggagt gctcctgcag
     3541 gttgtgacct tcaccaccga tgtaggaagt cacttcgaaa ccgttagtca gacgaacacg
     3601 gcatacttta cgcagcgcgg agttcggttt tttaggagtg gtagtatata cacgagtaca
     3661 tacgccacgt ttttgcgggc atgcttccag cgcaggcacg ttgcttttcg caactttgcg
     3721 agcacgtggt ttgcgtacca gctggttaac tgttgccatt aaatagctcc tggttttagc
     3781 ttttgcttcg taaacacgta ataaaacgtc ctcacacaat atgaggacgc cgaattttag
     3841 ggcgatgccg aaaaggtgtc aagaaatata caacgatccc gccatcacca ggccatctgg
     3901 ccggagtgct taaccgtaag tctgacgaaa tcagtatagt caatgggaat gatgtcgttc
     3961 gaaatttgac cagtcaaacc gcgggcaata aggtcttcgt tcagggcata gaccttaatg
     4021 ggggcattac gcagactttc aaggtagcgg ttaccgtcaa ctgcggcagt tacgccatct
     4081 tgcaataata gcagttcgtc gccttcactg agcagacgca gcagcgcagc aaaatccgtc
     4141 agccagggtg aacgatgtaa cgtgtgcagc ataaacgcct caaaacctca aaataacatc
     4201 gtagttggcg agttcgcggc gtaaggcatc tgcttcgagc ggcgtggctt cgacaacaaa
     4261 gggggtctgc ggatctaacc cgcgttcgcg cagtgaagcc gcacaaaccc agcactgttc
     4321 aatgtcgtac agatccaaca atttaaaagt ggcaatgtaa tcacgcgcca gcactgcatc
     4381 gggcttttgt cctggcagta gctgaaaaac gccatcagca ataaagaaga cggccagctc
     4441 gtcagttaac gcggaagttg ccagtaaagc atctaaacct tcccgcccgg cagcggtacc
     4501 atgaggcaca gtagaaaaaa caaacgcaat tcgtttcatc agaactgtac caccctgtca
     4561 caggtcagcg aggcttccgc cagcgcgcca agtccgctta aggtaaatcc ctgctgaagg
     4621 tttgacgaag ccagccccag tcttccggcc tccatttcat caacaacgcc acggcgtaat
     4681 gctgccgcta cgcagatatt cagcgccaca ccatgttgcg cattcaactg ttgccagccg
     4741 cgcacaaggt caaattcgtc gcttgccggt gaggtcaagt ggttagcgtt atagacccct
     4801 tcccgataga aaaagacgct gcttaactta tggccttctg ctatcagcgc ctgcgcaaac
     4861 tgaaaagcac tactcgcctg ttgcgtaccg tatgctggcc cggtcaccac gatggcaaaa
     4921 cgcattactt atcttgcccc tggaaatcac cgctcttgaa ctggcggatg tagagataga
     4981 cagtgtgttt ggagatgttc aggcggtcag caacctggtt gatcgcatct ttaatatcga
     5041 agatcccttt ctcgtagaga ttcagcacga tctgacgatt tttggcgtta ttagaaacat
     5101 tgcgatcggc attcacttct tcgatggtga actccagcgt ttgggtaacc agatcttcaa
     5161 cagaagaggc aaagttgacg cttgaaccga catccggggt ttctggcggc acaaaggtgc
     5221 tcataatctg cgagaaggga acatcaagat tcatattgat gcacagcaga ccaattacac
     5281 gctgttcgcg gttacgaatc gcgatagtca gggacttcat taatacgccg cttttggcgc
     5341 gagtaaagta gcatttagaa acgctgctat ccgctcccgt catatcgtgc agcatacgta
     5401 gcgccaggtc agtaattggc gaaccaatct tccggcctgt atgttcaccg ttagcaatgc
     5461 gaatggctga acattttaga tcctgcaaag agtgcaaaac gatttcacag tgggagccaa
     5521 taagcatcgc taacccgtcc accaccgctt cgtaggattt cagaatatca aaatcggtct
     5581 gctcgaaagg acgttgatcc agtaaatcca actcactggt ttcgttggtt aaaagcgacc
     5641 tggacatgaa aaaaaacact ccttttcagg agcctgtcgt taacttttca gggcaggctc
     5701 attaatgatg cgggtaacta aattaataca gcggaggttc cgctttccag cactaattat
     5761 atccggcctg taataaaaaa accgccgcct ggtcaggcgg cggttcttaa tgcttatttt
     5821 ttagcagaat ctgcggcttt cgcatcagct tccggctttg catcagcctt cggcgctggt
     5881 ttcacatcca gcagctctac gtcaaacacc agggtagaat tcggtgggat ccccggaaca
     5941 cccgctttgc cgtaagccag ttctggtgga ataaccagtt tgatcttacc gcctttcttg
     6001 atgttcttca gaccttctgt ccaacccggg ataacaccgt ccagacggaa agagagcggt
     6061 tcaccacggg tgtaagagtt gtcgaactct ttaccgtcga tcagcgtacc tttgtagttc
     6121 acgacaacag tatcgctgtc tttcggtgct tcgcctttac cggcttctac tacctgataa
     6181 accagaccag tggaagaggt tttcacacct ttctctttgg caaatttctc gcggtactct
     6241 ttaccttttg cttcgttatc agccgcgtct ttttccatct tcgcctgagc agaagacttc
     6301 acgcgagctt cgaatgcttg cagagtctgt tcgatctctt ggtcggagag tttgctctta
     6361 tcagcaaatg catcctgaac accagcgatc agctgatctt tatccagttt gatgcccagt
     6421 ttttcttgtt cttttagaga gttttccatg taacgaccca gcgaggcacc cagtgcataa
     6481 gctgatttct gatcgtcatt tttgaacgct gctttgctgt cagcagttgt agcaggtttt
     6541 gcagcttcag cagcaaaagt gattggtgca tgcaaggcaa cggccattgt ggtcgccagc
     6601 agcgttactt taaacagtga tttcatccat atctccagga tcggggcatc tcaccccagg
     6661 gttaactatt atcagaaggg tactataaag cgttgtcgaa caaatctaca tacagacacg
     6721 ccctattatc atctactttc agactctttt tgtttaaatt agtttcgatg accgcgaaat
     6781 gagtgctgtc tcgggcagca aagttaagta gaatccgcgg cgaccattcg acaaaagagg
     6841 tgaatcatgc aggatttatc attggaagca cgcctggcag agctggagag ccgactggct
     6901 tttcaggaaa tcaccattga agaactgaac gtcacggtga ccgctcatga aatggagatg
     6961 gcgaaactgc gcgatcatct gcgtctgctg accgagaagt taaaagccag ccagccgtcg
     7021 aacatcgcgt cgcaggctga agaaacgcca ccgccacatt attgaggcgt aaaaaaagcg
     7081 gggattcccc gcttttttgt caccttttcg gtattagtgg caaccgcaac cgccgttgcc
     7141 tttaccgcca cagcagcctt cgccaccgtg ttcatgaccg tgatcgtggc catgaccgcc
     7201 gcagcaaccg tcgtggtcgt gatcgtggtg atgatcgtgc gcgccgtgaa cgtggccatg
     7261 agccagttct tcttcggtcg cttcgcgaat cgccacaact tcaacgttga atttcaggtt
     7321 ctgaccggcc agcatgtggt taccatcaac cacgacgtga tcgtcttcaa ccgcagtgat
     7381 ttcaaccggt accggaccct ggtcggtttc agccaggaaa cgcataccta cctgcagttc
     7441 atcaacgccc ataaatacgt ctttaggaac acgttgcacc aggttttcgt cgtactgacc
     7501 gtaagcgtcg ttcgcgccaa cagcgacatc aaatttgtcg ccaacttcat gaccttccag
     7561 cgccgtttcc aggccagaga tcagggaacc gtgaccatgc aggtagtcca gcggcgcact
     7621 caccggagac tcatcaacca acacaccgtc ttctgtacgt acctgatagg ccaggctgac
     7681 caccaggtct tttgctactt tcatgatatc tcctgagcat gggaagaata gtggcgcaga
     7741 ttgtagcgga attctgcagc cgtgtactca ctagcttaaa aaaactcggc gcatatcgct
     7801 agtccggatg aaaaatcccg atcacttgct catctttgcg aacgtgatcg cgggcttctt
     7861 tgtctgcttc tcgcatctgg tgtccgcact taacgcattc aacaatatca atattatttt
     7921 cgcgccacat cgccattgaa tcctgcgcct gacaggccgg gcattttgcg cccgcaataa
     7981 aacgttttcg gattgccatc tttaccctct actcaaattc atcccagccg tccagctagc
     8041 gtcgttcttg ttgcatttca cgctggaaaa tctcttccag ttcgcgtcgg gcttccctgg
     8101 cgcgagaaat ttgtacggta tcggcatgca ttgggataag ctctcgcagc atccgcatat
     8161 ccaggcggcg aaaatgcagt tgcgcccgct gtgcctgatg cggatgcata ccaagcgtga
     8221 ccagcgtctt gcgccccagc tctaacgcac tggagaatgt ttcacgggaa aactgcgtca
     8281 cccctgcctg taataactca tgcgcttcca cacgtccgcg cgctcgcgca agaatatgca
     8341 aatgcggaaa gtgctgctgg catatttcca ccagcttcat ggtgtcttcc ggctcgttac
     8401 aggtaatgac gatagactca gcggcctctg cacccgcaga acgtaaaaga tctacctgcg
     8461 tggcatcacc gtaataaact ttgtagccat atttgcgcat caggttaacg gcgctgatat
     8521 cccgctccag cacagtaatg cgcattttat ttgccatcaa caaacgacca atcacctgcc
     8581 caaaacgtcc aaagcccacg acaatgacct ggggtttatc atcgttgacc cacggttttt
     8641 cgtcttcttc ttccggtccg ttaaactggc gggatagcca tttatccacc agcttcatca
     8701 gcagcggcgt ggtcatcatg gaaagcgtca ccgtcaccag caacagcgcc atctggtcgc
     8761 cctggaataa gcgttgtgaa gaagcggtag aaaagaggac aaaggcaaac tcaccgccct
     8821 gactcaacac gccagcaaac tgcatccgct ctgagctacg cacgccatac aatcgcgcca
     8881 gcagatacag cacgagaatt ttcaccgcca ccagcacaac cacgcttatc actacccaca
     8941 acagatgggt ataaagcacc ccgaggttga gtgacatccc gacagagata aagaacaaac
     9001 cgagcagcaa gcctttaaag ggatcgatag ccgtttccag ttcatggcga tattcacttt
     9061 ccgccagcag cacgcctgca ataaacgtac cgagcgccat cgacagcccc aacgcatcca
     9121 taaacaatgc ggaacccaat accagcagca gcgtcgcggc ggtgaacact tcccgcacgc
     9181 cagaagctgc aataaaacgg aataccggac gcaataaata gcgcccgcca atcagcatgc
     9241 cgacaaacgc cagcaccttc atgccgatct tcatccagtc gaaatgttcg tctgccgaac
     9301 ccgccaacaa cggcactaac gccagtgccg ggatcaccgc cagatcctga aacagcagta
     9361 ccgaaaaccc gagctgcccg gattcgctgc gattcatccc tttctcacgc atcaattgca
     9421 acgccattgc ggtcgaagac atcgcaaggc caatgccacc gaccaccgcc gcctgccagg
     9481 cgaaatccgt cagcatcaat aatcccgcca gcaacgccgc gcttaacagc acctgtgccg
     9541 cgcctacgcc aaaaatcgaa cgccgcagtt gccaaagctt ggagggattc aactcaaggc
     9601 cgatgataaa catcaggaac accacgccga gttccgaaaa gtggaggatc tcatcgacgt
     9661 cgctaataaa ccccagcccc cacgggccaa ttgcaatccc tgccagcaaa tatcccaaca
     9721 cagcgccaat acccagccgc gatgccaacg gcaccgcagc caccgccgcg aagagaaaca
     9781 gcactcctgc aagtaaaaaa tcggaacctt ccatcagcgg cctcctggag acagcggatt
     9841 tgccagccag tcaccgtagg ctctggcgtg gctcgccagc tcctgtgcgc tttgccgtct
     9901 cgcccagtaa ataatgatgg gacttaacca atgcatccgg cacatgcccg ccgccagttc
     9961 aaaggggcgc aggacatcgc tcatcgggta acgattcaac gcgtcataac ggtaagcact
    10021 ttccggctcg ccggtggtaa tcacactacg ccagtacttt cccgccagtt ggtttcctcc
    10081 cggcccgctg gcaaaaccac ggcttaatac ccggtccagc cactctttca gtagcgccgg
    10141 gcagctatag gtataaagag gatgctgaaa gacaatcacc tcgtgctcgc gcagtaatgc
    10201 ctgctcacgg gggatatcaa taaaaaaatc gggatagtgc gcgtaaaggt cgtgcacggt
    10261 aacattgctg agctgcgtgg ccggtttaag cagtacccgg tttgccaccg agtcctgaga
    10321 ttccggatgg gcatacagca gcaaaacttt cgctggctga gacatcatcc ccctcccggt
    10381 atggtttttg tgtatagtcg ctgttttggg ctaccattgc gcccggtgcg gcagctcgcc
    10441 catacattac attatcataa tgataagtta acatagtctg aacatacggc accttatgat
    10501 tgttttctcc tcgttacaaa ttcgtcgcgg cgtgcgcgtc ctgctggata atgccaccgc
    10561 caccatcaac cccgggcaga aagtcggcct ggtgggtaaa aacggctgtg gtaaatctac
    10621 cctgctggca ttgctgaaaa atgaaatcag cgccgacggc ggcagctaca cctttccggg
    10681 aagctggcaa ctggcgtggg tgaatcagga aacgccggcg ttaccgcaag cggcgctgga
    10741 atatgtcatt gacggcgacc gtgaatatcg tcaactggaa gcgcagctac acgacgccaa
    10801 cgaacgtaac gacgggcacg ccattgcgac cattcatggc aagctggatg ctattgacgc
    10861 atggagtatt cgctcccgtg ccgccagcct gctgcacggc ctcggtttca gcaatgaaca
    10921 actggagcgc ccggtaagtg atttctctgg tggctggcgt atgcgtctta accttgccca
    10981 ggcgctgatt tgccgttcag acttgctgct gctcgacgaa ccgactaacc acctcgatct
    11041 cgatgccgtt atctggctgg aaaaatggct gaagagctat cagggcacgc tgatcctgat
    11101 ctctcacgac cgcgacttcc tcgatccgat cgtcgataaa attattcata tcgaacaaca
    11161 aagcatgttc gagtacaccg gcaactacag ttcgtttgaa gtacagcgcg ccacccgtct
    11221 ggcgcagcaa caagcgatgt acgaaagcca gcaggaacgc gtagcgcatc tgcaaagtta
    11281 tatcgaccgt ttccgtgcca aagccaccaa agcgaagcag gcccagagcc gcattaagat
    11341 gctcgagcgt atggagctga ttgcccccgc gcacgtcgac aacccgttcc gctttagctt
    11401 ccgcgcgccg gaaagcctgc caaatccgtt actgaagatg gaaaaggtca gcgcaggcta
    11461 tggcgatcgc attattctcg actcgattaa actgaatctg gtccccggtt cgcgcattgg
    11521 tctgttaggc cgcaacggcg cgggtaaatc gacattaatc aaactgttag ccggtgaact
    11581 tgcgccagtc agtggtgaga taggcctggc aaaagggatc aagctcggct acttcgccca
    11641 gcatcaactt gaatacctgc gcgccgacga atcgcctatt caacatctgg cacgtttagc
    11701 gccgcaggaa ctggagcaaa aactgcgtga ctacctcggc ggctttggtt tccagggcga
    11761 taaagtaacc gaagaaacgc gccgcttctc cggtggggaa aaagcccgcc tggtgctggc
    11821 attaatcgtc tggcagcgtc cgaatctgct gctgctcgac gaaccgacta accaccttga
    11881 ccttgatatg cgtcaggcac tcaccgaagc attaatcgag tttgaaggcg cgctggttgt
    11941 cgtttcgcac gaccgtcatt tgctgcgttc caccactgac gatctctacc tggttcacga
    12001 tcgtaaagtc gaaccgttcg acggcgatct ggaagattat caacagtggt tgagcgacgt
    12061 acaaaagcag gaaaaccaga ccgacgaagc gccaaaagag aacgcgaaca gcgcccaggc
    12121 acgtaaagat cagaagcgtc gggaagcgga gctgcgtgcg caaacccagc cactgcgtaa
    12181 agagattgcc cgtctggaaa aagagatgga gaagctgaac gcgcaactgg cgcaggcaga
    12241 agagaaactc ggcgacagcg aactgtatga ccagagccgt aaagcggagt tgaccgcctg
    12301 cctgcaacag caagccagcg ccaaatccgg cctggaagag tgcgaaatgg catggctgga
    12361 agcccaggag cagcttgagc agatgctgct ggaaggccaa agcaactgat ggcgcagata
    12421 acgacgaccg atgccaatga attcagcagc agtgctgaat tcacccctat gcgcggcttt
    12481 agcaattgtc atctgcaaac catgctgccg cgtctgtttc gtcgcaaggt gaaattcacc
    12541 ccgtactggc agcgactgga gttgcccgac ggcgattttg tcgatctcgc gtggagtgaa
    12601 gcccctgcac aggcgcaaca taaacctcgt ctggtggtgt ttcacgggct ggaaggcagt
    12661 ctcaatagcc cttacgccca cggtctggtt gaggcggcgc aaaagcgcgg ctggctgggc
    12721 gtggtgatgc attttcgcgg atgcagcggt gaaccaaacc gtatgcaccg catttaccat
    12781 tcgggcgaaa ccgaagacgc cagttggttt ttacgctggc tgcaacgcga gtttggccat
    12841 gcgccaacgg ctgccgtcgg ctattcgctc ggcggtaata tgctggcctg tttgctggca
    12901 aaagaaggca atgatctccc ggttgatgcg gcggttattg tctctgcgcc gtttatgctg
    12961 gaagcctgta gctatcatat ggaaaagggc ttttcccgcg tttatcagcg ttacttgctg
    13021 aacctgttaa aagccaatgc cgcgcgcaag ctggcagcct accccggaac actgccgatt
    13081 aatctcgcgc agttaaaatc ggtacgtcgc atccgtgaat ttgacgatct catcaccgcc
    13141 agaattcacg gctacgctga cgctatcgac tattatcgtc agtgtagcgc catgccgatg
    13201 ctgaaccgga tcgccaaacc gacgctgatt attcacgcca aagacgatcc gtttatggat
    13261 catcaggtga tcccgaaacc ggaaagtctc cccccgcagg tggagtatca actgactgaa
    13321 catggcggtc atgttggctt tattggcggt acattacttc atccgcaaat gtggctggag
    13381 tcacgcattc ctgactggtt aacaacgtat ctggaggcga aatcatgttg attccgtggc
    13441 aagacctctc ccccgaaacg ctggaaaatt taattgaaag ctttgtgtta cgtgaaggca
    13501 ccgattatgg tgaacatgag cgtacacttg aacagaaagt cgccgacgtc aaacgccagc
    13561 tacagtgcgg agaagcggtg ctggtatggt cggagctgca cgaaacggtc aatatcatgc
    13621 cgcgcagtca gtttcgcgaa taacctgcaa ccgtcggcgg acgagataat aatgatagct
    13681 aagtcatgga gttactatgt ctgccaaaca tccggtcatt gcggtaacag gatccagcgg
    13741 cgcggggacc accaccacca gcctcgcgtt tcgtaaaata ttcgcgcagt taaatctgca
    13801 tgcagccgag gtggaaggcg acagttttca ccgttacacc cgcccggaaa tggacatggc
    13861 gatccgcaaa gcgcgcgacg ccgggcggca tatcagctac ttcggccccg aagcgaacga
    13921 cttcggcctg ctggaacaaa ccttcattga atacggtcag agcggcaaag ggaaatctcg
    13981 caaatatctg catacctacg atgaagccgt accgtggaat caggtaccgg gaacattcac
    14041 cccctggcaa cctttaccag aacccactga tgtactgttt tatgaaggtt tacacggcgg
    14101 cgtagtcacg ccacagcata acgttgcgca gcatgtggac ttactggtcg gcgtggtacc
    14161 tatcgttaac cttgagtgga ttcaaaaact gatccgcgac accagcgagc gcgggcactc
    14221 acgagaagca gtgatggatt cagtagtgcg ttcaatggaa gactatatca actacatcac
    14281 accgcagttt tcccgcaccc atcttaactt ccagcgcgtt cccaccgtcg acacttcaaa
    14341 cccgttcgcg gcaaaaggta tcccgtcgct ggatgaaagc tttgtggtga tccattttcg
    14401 taatctggaa gggatcgatt tcccctggct gctggcgatg ttgcaaggct cattcatttc
    14461 ccacatcaat acgttagtgg tgcccggcgg caaaatgggt ctggcaattg aattaattat
    14521 gctgccgctg gtgcaacgat tgatggaagg aaagaaaatc gagtaactct gctattacgc
    14581 cggataaaat actatccggc ttcacaacgg gatagttaaa tcacgcggca accacttcat
    14641 acgagtgagt aatattcacc gctttttcca gcatcaacgc cactgaacaa tatttctcag
    14701 cagagagatc aaccgcacgc gcaaccgctg cgtctttcag gtcgcgaccg gtgacgataa
    14761 aatgcagatt aatgtgcgta aacaggcgtg gtgcctcttc gcggcgttca gaggtcaatt
    14821 ttacttcaca atcgaccaca tcctgacgcc ctttttgcag gatcgaaacc acatcgatgg
    14881 cactgcaacc acccgccgcc atcaacacca tttccatcgg acttggtgct ttatcgcctg
    14941 agttgccgtc cattaaaatc tggtgaccag aggcagattc gcccagaaaa gttaaccctt
    15001 cgacccactt cactcgcgct tgcatttttg ctactccact gcgtcaattt tcctgacaga
    15061 gtacgcgtac taaccaaatc gcgcaacgga aggcgacctg ggtcatgctg aagcgagaca
    15121 ccaggagaca caaagcgaaa gctatgctaa aacagtcagg atgctacagt aatacattga
    15181 tgtactgcat gtatgcaaag gacgtcacat taccgtgcag tacagttgat agccccttcc
    15241 caggtagcgg gaagcatatt tcggcaatcc agagacagcg gcgttatctg gctctggaga
    15301 aagcttataa cagaggataa ccgcgcatgg tgcttggcaa accgcaaaca gacccgactc
    15361 tcgaatggtt cttgtctcat tgccacattc ataagtaccc atccaagagc acgcttattc
    15421 accagggtga aaaagcggaa acgctgtact acatcgttaa aggctctgtg gctgtgctga
    15481 tcaaagacga agagggtaaa gaaatgatcc tctcctatct gaatcagggt gattttattg
    15541 gcgaactggg cctgtttgaa gagggccagg aacgtagcgc atgggtacgt gcgaaaaccg
    15601 cctgtgaagt ggctgaaatt tcgtacaaaa aatttcgcca attgattcag gtaaacccgg
    15661 acattctgat gcgtctgtct gcacagatgg cgcgtcgtct gcaagtcact tcagagaaag
    15721 tgggcaacct ggcgttcctc gacgtgacgg gccgcattgc acagactctg ctcaatctgg
    15781 caaaacaacc agacgctatg actcacccgg acggtatgca aatcaaaatt acccgtcagg
    15841 aaatcggtca gattgtcggc tgttctcgtg aaaccgtggg acgcattctg aagatgcttg
    15901 aagatcagaa cctgatctcc gcacacggta aaaccatcgt cgtttacggc actcgttaat
    15961 cccgtcggag tggcgcgtta cctggtagcg cgccattttg tttcccccga tgtggcgcag
    16021 actgatttat caccccgata ttaactatgc actgcgacaa acgctggtgc tatgtttgcc
    16081 cgtggccgtt gggttaatgc ttggcgaatt acgattcggt ctgctcttct ctctcgttcc
    16141 tgcctgttgc aatattgcgg gccttgatac gcctcataaa cgttttttca aacgcttaat
    16201 cattggtgcg tcgctgtttg ccacctgtag cttactgaca cagctgctac tggcaaaaga
    16261 tgttcccctg ccttttttgc tgaccggatt aacgctggta cttggcgtca ctgctgagtt
    16321 ggggccattg cacgcaaaat tgcttcccgc atcgctgctg gcagccattt ttaccctcag
    16381 tctggcggga tacatgccgg tctgggaacc gttgctcatc tatgcgttgg gcactctctg
    16441 gtacggattg tttaactggt tttggttctg gatctggcgc gaacaaccgc tgcgcgagtc
    16501 actaagtctg ctgtaccgtg aactggcaga ttattgtgaa gccaaataca gcctgcttac
    16561 ccagcacacc gaccctgaaa aagcgctgcc gccgctgctg gtgcgccagc aaaaagcggt
    16621 cgatctaatt acccagtgct atcagcaaat gcatatgctt tccgcgcaaa ataatactga
    16681 ctacaagcgg atgctgcgta ttttccagga ggcgctggat ttacaggaac atatttcggt
    16741 cagtttgcat cagccggaag aggtgcaaaa gctggtcgag cgtagccatg cggaagaagt
    16801 tatccgctgg aatgcgcaaa ccgtcgccgc tcgcctgcgc gtgctggctg atgacattct
    16861 ttaccatcgc ctgccgacgc gttttacgat ggaaaagcaa attggcgcac tggaaaaaat
    16921 cgcccgccag catccggata atccggttgg gcaattctgc tattggcact tcagccgcat
    16981 cgcccgcgtg ctgcgcaccc aaaaaccgct ctatgcccgt gacttactgg ccgataaaca
    17041 gcggcgaatg ccgttacttc cggcgctgaa aagttatttg tcactaaagt ctccggcgct
    17101 acgcaatgcc ggacgactca gtgtgatgtt aagcgttgcc agcttgatgg gcaccgcgct
    17161 gcatctgccg aagtcgtact ggatcctgat gacggtattg ctggtgacgc aaaatggcta
    17221 tggcgcaacc cgtctgagga ttgtgaatcg ctccgtggga accgtggtcg ggttaatcat
    17281 tgcgggcgtg gcgctgcact ttaaaattcc cgaaggttac accctgacct tgatgctgat
    17341 taccaccctt gccagctacc tgatattgcg caaaaactac ggctgggcga cggtcggttt
    17401 tactattacc gcagtgtata ccctgcaact gttgtggctg aacggtgagc agtacatcct
    17461 tccgcgtctt atcgatacca ttattggttg tttaattgct ttcggtggta ccgtctggct
    17521 gtggccgcag tggcagagcg ggttattgcg taaaaacgcc catgacgctt tagaagccta
    17581 tcaggaagcg attcgcttga ttctgagcga ggatccgcaa ccaacgccac tggcctggca
    17641 gcgaatgcgg gtaaatcagg cgcataacac tctgtataac tcattgaatc aggcgatgca
    17701 ggaaccagcg ttcaacagcc attatctggc agatatgaaa ctgtgggtaa cgcacagtca
    17761 gtttattgtt gaacatatta atgccatgac cacgctggcg cgggaacacc gggcattgcc
    17821 acctgaactg gcacaagagt atttacagtc ttgtgaaatc gccattcagc gttgtcagca
    17881 gcgactggag tatgacgaac cgggtagttc tggcgatgcc aatatcatgg atgcgccgga
    17941 gatgcagccg cacgaaggcg cggcaggtac gctggagcag catttacagc gggttattgg
    18001 tcatctgaac accatgcaca ccatttcgtc gatggcatgg cgtcagcgac cgcatcacgg
    18061 gatttggctg agtcgcaagt tgcgggattc gaaggcgtaa tgcaggctaa accatcggat
    18121 aaggcatgca cgccgcatcc gacatctttt gcctgatgcg tcttatttac gccccaacca
    18181 ccttcgccac cgcgtgggcg aaacgttgca tcccttcatc gatatccgca tcttccacca
    18241 ccagcgacgg cgcaaaacgc atcacatccg gtccggcatt cagcaccatt acgccagcct
    18301 ctgcgcccgc atacaggaaa tcacgcgcct gacctttgta ctgtggtttc agctccgcgc
    18361 caattaacag ccccataccg cgaatatcgc taaacacatc gtactgctga tcgatcttct
    18421 gcaaatggtc aacaaaacgc tggcgtttcg cctgaatgcc ttccagcact tcaggggtat
    18481 taatgatatc aaacgccgcg cccgctactg cacaggccag aggattaccg ccgtaggtgg
    18541 aaccgtgaga accaggatga aacgcagaag caatttccgc cgtggtcagc atggcgctaa
    18601 tcgggaagcc gccgcctaac gctttcgcag aagtcagaat atccggcgtc acgccgtagt
    18661 gcatgtaagc aaacaaatcg ccagtccgcc ccatcccgca ctgtacttca tcaaacacca
    18721 ataatgcctg atgttgatcg cacaactcac gcaagccctg caaaaactct ggcgtcgctg
    18781 ccgtcacacc gccctcgccc tggatcggct caaccaccac cgcacaggtg tgatcatcca
    18841 tcaccgcttt cactgcgtgg agatcgttaa agggaacgtg gatgatgtct gatggttttg
    18901 gcccaaagcc gtcggaatat tttggctgcc cacccaccga aacggtaaac agcgagcgac
    18961 catgaaaagc gttatggaag gcaataattt tggttttgaa cgggctatga cgcacgcagg
    19021 cgtaatggcg tgccagttta aaggcggttt cgttagcttc cgtgccggag ttcataaaca
    19081 ccacgcgttc ggcaaacgtc gcctcaatca gtttacgccc aagacgcagc gccggttcat
    19141 tggtgaaaac gttactgata tgccacagag tttcgccctg ggtttttaac gcgttcacca
    19201 gcgcaggatg gcaatggccc aacgccgtaa ctgcaatgcc acccgcgaaa tcgacatact
    19261 ccttgccttg ctgatcccag attcggctgc cctgaccttt taccggaatg aactctgccg
    19321 gagcataaat cggcaggatc acttcatcga aagtcgcgcg tgtaattgct gtttgttcaa
    19381 ttgccatctc atgatcaccc tgttatgcat aaataaatgt gaaattataa tcacaaaata
    19441 tgcataaaaa atcattgaat ggcaatcaga aatcagcgat gcaggaaatt agccagcagt
    19501 tgatgtcctt gttcgctaag aatactttct ggatggaact gcacaccttc cagatcccac
    19561 tggcgatggc gaatccccat aatctcgcgg gtttcgctcc aggccgtcac ttcaaagcac
    19621 gctggtaacg agtcaggttc cactaccagc gaatgatagc gtgtcacggt aagtggattt
    19681 gccagccccc gaaatacgcc ctcaccgtta tgtgtaatcg gcgaggtttt gccgtgcatg
    19741 acctttgcgg cgcgcacaac tttaccgcca aatgcctgcg ccattgcctg atgaccgagg
    19801 cagacgccaa gaatcggcaa gcgcccggca tagtggcgaa taacgtcaag ggagatcccg
    19861 gcttcatctg gcgtacaggg gccgggtgag atgacaactt tttgtggttt aagggcgtcg
    19921 atatccgcca gcgtcaacgc atcgttgcgc ttaaccagca catccgcccc cagttcacaa
    19981 aagtactggt agaggttcca ggtaaaagaa tcgtagttat ctataagcag gatcatggcg
    20041 gctccggtac gaaagaaccg cgctatttta ctcagattcc ccggcttcgc ttaccacttt
    20101 gctaaatatc atctgcaacg cggtgagatc ccccattgcc ccactctgat ttgcctgatt
    20161 ccaggcctct ttttcgatac cctgccagct cagttgatat ccggcatgaa tcgccagttg
    20221 ctcgaagaag atccgctgtg ccagaccact tcccacccgg aagggatgca gcacgttgat
    20281 ttcacaatag taatgcgcca gccgctcgac gaacttcgtt ttctccaggc caaccagata
    20341 accttcttcc tccagatcct gcatcagggc attgccctct ttttcgatat aagcaaagtg
    20401 gcagaacggc gtatcgccct gataaatatc aacttcacgc agttgccctg cccagtcgaa
    20461 aatatcctga tacagctggc gatggatagt gcgcaaatgc ggtaaaccgc gcaccagcgg
    20521 accaagctca atggtcgcag cacgcagcgc cgtcatttcg taagcggcct gttccagccg
    20581 ttgttgctgg cggatgttca gccggttacg catgatatca aggccaggat aaagatacgg
    20641 atcgcgccct tcgccgaatt tatcgctcat agtgactcct cagctcttca aggcgcgcta
    20701 aagcctctgc ggcagtaaga gtgactaaag gcatctcgac gccttcaaga cggcgactgg
    20761 cctgaaaatt acgattacgc tgaagctccc agagacggga cttttgctta tcggtgagtt
    20821 tcttcacttg atgcctccct gaacgtgtcc atttgccaca agtataagcg gcaaatccgg
    20881 gttacgccgg gagagcagaa gcgcgggcaa gattgcccgc gcgagaaatc attacggcag
    20941 gactttagcg gaaaggataa ctaccggttt tgacggcaca ttctggtacg gaccaacgtc
    21001 atgagtcggc acctgggaaa tcttatcggc aacgtccatg cctttcacca ctttaccaaa
    21061 taccgcgtaa ccgaaatcac gctgaccatg gtcaaggaag gcgttatcgg caacgttgat
    21121 aaagaactgg ctggtggcgc tgtctttgtc agcggtacgc gccatcgcga tggtgccacg
    21181 cgtgttgcga aggccgttat cggcttcatt tttgattggc gggtttggtt ttttctgctg
    21241 catctgctcg gtgaaaccgc cgccctgaat cataaagcca ggaatgacgc ggtgaaaggt
    21301 agtgttgtta taaaaaccgc tgttcacata atcgacaaag ttttgcacag acactggcgc
    21361 tttttgttta tccagctcca gttcgatgtt accagctgag gttgtcaaca atacgtgcgg
    21421 gtcccctttc gctgccattg ctgcgggaga aagagcagaa agagcgaaaa cagcagccat
    21481 cgccgccagg gtcgatttga acatgagatt tccttaacaa acagagcaga aaaaaagcaa
    21541 gtgaagagat tctaaatagc cttcaagaac aaggccatcc ctttacctaa ttttacgtat
    21601 ctgaaatata tgtaacctga cgatcacttt ttattccgtg atcaaaatca cctcttaaaa
    21661 tgcaatttag caaccgattg caataaaaca tttaaacaga tcacaaaatc acctaaaatc
    21721 gcccgctcgc ggcatatccc gctatgccat tttatttttg aacgcaggcc aatcatgact
    21781 aacagcaatc gcatcaagct cacatggatt agctttctct cctacgcact gactggtgcg
    21841 ttggttattg tcaccgggat ggtgatggga aatatcgccg attatttcaa tctgcctgtt
    21901 tccagtatga gtaacacctt caccttcctc aacgccggca ttttaatctc tatcttcctc
    21961 aacgcctggc tgatggaaat cgtcccgttg aaaacgcagt tacgttttgg ctttctcctg
    22021 atggtgctgg cggttgccgg tttgatgttc agccacagcc tggcgctgtt ctcggcggcg
    22081 atgttcattc tcggggtggt cagcggcatc accatgtcga ttggtacatt cctggtaaca
    22141 caaatgtatg aagggcgtca gcgcggttcc cgcctgttat ttaccgactc cttcttcagt
    22201 atggctggga tgattttccc aatgatcgcc gcgtttctac tggcgcgcag cattgagtgg
    22261 tactgggttt atgcctgcat cgggctggtg tatgtcgcta tttttattct gaccttcggc
    22321 tgtgagttcc cggcgctggg caaacatgca ccaaaaacgg atgctccggt agcgaaagaa
    22381 aagtggggga tcggcgtact gtttctctct gttgcggcgc tgtgctacat cctcggtcag
    22441 ttaggtttta tctcctgggt gcctgagtat gccaaaggcc tgggcatgag cctgaatgat
    22501 gctggcacgc tggtgagtaa cttctggatg tcatacatgg tcggcatgtg ggcgttcagc
    22561 tttattcttc gcttctttga tttgcaacgc attctgaccg tactggctgg tctggctgcg
    22621 attctgatgt acgtctttaa caccggaaca ccggcacata tggcgtggtc aattctcgcc
    22681 ctgggcttct tctccagcgc gatctatacc accatcatca ccctgggttc acagcagacc
    22741 aaagtaccgt cgccaaaact ggttaacttt gtcctgacct gcggaaccat cggtactatg
    22801 ttgacctttg tggttaccgg cccgattgtt gaacatagcg gtccgcaggc ggcactgctt
    22861 acagcaaacg gtctgtacgc tgtcgtcttt gtgatgtgct tcctgttagg tttcgtcagc
    22921 cgtcaccgtc agcataacac cctgacctct cattaattgc tcatgccgga cggcactatc
    22981 gtcgtccggc cttttcctct ctaaccccgc tacgtgcatc tatttctata aacccgttca
    23041 ttttgtctat tttttgcaca aacatgaaat gtcagacaat tccgtgactt aagaaaattt
    23101 atacaaatca gcaatatacc ccttaaggag tatataaagg tgaatttgat ttacatcaat
    23161 aagcggggtt gctgaatcgt taaggtaggc ggtaatagaa aagaaatcga ggcaaaaatg
    23221 agcaaagtca gactcgcaat tatcggtaac ggtatggtcg gccatcgctt tatcgaagat
    23281 cttcttgata aatctgatgc ggccaacttt gatattaccg ttttctgtga agaaccgcgc
    23341 atcgcttatg accgcgtaca cctctcgtct tacttctctc accacaccgc cgaagagctg
    23401 tcgctggtgc gcgaaggctt ctacgagaaa cacggcatca aagttctggt cggcgaacgc
    23461 gctatcacca tcaaccgtca ggagaaggtg attcactcca gcgccggacg taccgttttt
    23521 tatgacaagc tgatcatggc gaccggttcc tacccgtgga tcccgccaat taaaggttct
    23581 gatactcagg actgctttgt ctatcgcacc attgaagacc tcaacgccat tgaatcctgc
    23641 gcccgtcgca gcaaacgcgg tgccgttgtt ggtggcggcc tgttaggtct ggaagccgct
    23701 ggcgcgctga aaaacttagg tattgaaacc cacgttatcg aatttgcccc tatgctgatg
    23761 gcagaacagc ttgatcagat gggtggcgag cagctgcgtc gcaaaatcga gagcatgggc
    23821 gtgcgcgttc acaccagcaa aaacaccctt gagattgtgc aggaaggtgt tgaagcgcgt
    23881 aaaaccatgc gttttgccga cggcagcgaa ctggaagtcg actttatcgt cttctctacc
    23941 ggtatccgtc cgcgcgataa gctggcaacc cagtgtggtc tggacgttgc tccgcgtggg
    24001 ggtattgtca ttaatgattc ctgccagact tccgatccgg atatctacgc catcggtgaa
    24061 tgcgcaagct ggaacaaccg tgtatttggt ctggtagcac caggctacaa aatggcgcag
    24121 gtcgccgttg accatattct cggtagcgaa aacgcctttg aaggtgctga ccttagcgcc
    24181 aagctgaaac tgctgggcgt agacgtaggc ggtattggtg atgcgcacgg tcgcacgcct
    24241 ggcgcacgta gctacgttta cctcgacgaa agcaaagaga tctacaaacg ccttattgtc
    24301 agcgaagaca acaaaaccct gctcggtgcg gtactggtgg gcgataccag cgactacggc
    24361 aacctgctgc aactggtgct gaatgctatc gagctgccgg aaaacccgga ttctctgatt
    24421 ctgccggcgc actcgggtag cggtaagccg tctatcggtg ttgataaact gccggacagc
    24481 gcgcaaatct gctcctgctt cgacgtcacc aaaggtgatc tgattgctgc catcaacaaa
    24541 ggctgtcaca ccgttgcggc gctgaaagcc gaaaccaaag cgggtactgg ctgcggtggc
    24601 tgtatcccgc tggtcactca ggtactgaac gcggaactgg cgaaacaggg catcgaagtt
    24661 aacaacaacc tgtgcgaaca ctttgcttat tcgcgtcagg aactattcca tttgatccgc
    24721 gttgaaggca ttaaaacctt cgaagaactg ctggcgaaac acggcaaagg ttacggttgt
    24781 gaagtttgta aaccgaccgt cggttcgctg ctggcctcct gctggaacga atacattctg
    24841 aagccggaac atactccgct gcaggattct aacgacaact tcctcgctaa catccagaaa
    24901 gacggcacct actcggtgat cccgcgctct ccgggcggtg aaatcacccc ggaagggctg
    24961 atggcggtag gtcgtatcgc gcgtgaattt aatctctaca ccaagatcac tggctcccag
    25021 cgtctggcga tgtttggcgc acagaaagac gatctgccgg agatctggcg tcagctgatt
    25081 gaagccggct tcgaaaccgg tcatgcctat gcgaaagcac tgcgtatggc gaaaacctgc
    25141 gtgggtagca cctggtgccg ctacggcgtt ggcgacagcg tcggcctcgg cgtggaactg
    25201 gaaaaccgct acaaaggcat ccgtacgccg cacaaaatga agttcggtgt ctccggctgt
    25261 acccgtgaat gttcagaagc tcagggtaaa gacgtgggta ttatcgccac tgaaaaaggc
    25321 tggaacctgt atgtttgcgg taacggcggc atgaaaccgc gtcatgcgga tctgctggcg
    25381 gcggatatcg atcgcgaaac gctgatcaaa tatctcgacc gcttcatgat gttctacatc
    25441 cgtactgccg acaaactgac gcgtaccgca ccgtggttag aaaacctcga aggcggcatc
    25501 gattacctga aagcggtgat cattgacgac aaactggggc tgaacgcaca tctggaagaa
    25561 gagatggcgc gcctgcgtga agcggtagtg tgtgagtgga ctgaaacggt caatacgccg
    25621 tctgcgcaga ctcgcttcaa acacttcatc aacagcgaca agcgtgaccc gaacgtgcag
    25681 atggtgccag agcgcgaaca gcaccgtccg gcaacgccgt atgaacgtat cccagtaact
    25741 ctggtggagg acaacgcatg agccagtgga aagacatctg caaaatcgat gacatcctgc
    25801 ctgaaaccgg cgtctgcgcg ctgttaggtg acgagcaggt cgcgattttc cgcccgtatc
    25861 acagcgatca ggtgtttgcg atcagcaaca tcgacccgtt cttcgagtcc agcgtgctgt
    25921 cacgcggact gattgcggaa caccagggcg agctgtgggt cgccagcccg ctgaaaaaac
    25981 agcgttttcg cttaagcgac ggcttgtgca tggaagacga acagttttcc gtcaaacatt
    26041 acgaagcgcg agtgaaagac ggcgtggtgc agctgcgcgg ttaatgtttt aacgggaggc
    26101 gcaatgcctc ccctttttgc atggtcctgt aataaccttc ggtatattgc aggacatttt
    26161 ttaaactttt tgttttattt tttgttttta ttttttaaag gataatcaaa tgtttacaga
    26221 cactattaat aagtgtgcgg ctaacgctgc gcgcattgca cgcctgtcgg caaataaccc
    26281 gctcggcttt tgggtcagct ccgccatggc gggcgcgtat gtgggtcttg ggatcatcct
    26341 gatttttacg ctcggtaatt tgctcgatcc atccgtacgc cctctggtga tgggcgcgac
    26401 ctttggtatc gccttaacgc tggtgattat cgccggttct gagctgttca ccggacacac
    26461 catgttcctc acctttgggg tgaaagcggg caccatcagc cacgggcaaa tgtgggcaat
    26521 cctgccgcaa acctggctgg gtaacctggt cggttccgtc ttcgttgcca tgctctatag
    26581 ctggggtggt ggtagcctgc tgccggtaga taccagcatc gttcactccg tcgcgctggc
    26641 taaaaccact gcaccggcaa tggtactgtt cttcaaaggt gcattgtgta actggctggt
    26701 ttgtctggca atctggatgg cgctgcgcac tgaaggggcg gcgaaattta tcgcaatctg
    26761 gtggtgtctg ctggcattta tcgcgtccgg ctacgagcac tccatcgcta acatgacgct
    26821 gttcgcgctc tcctggttcg gcaaccacag cgaagcctac acgctggcgg gtattggtca
    26881 taacctgctg tgggtgacgc tgggtaatac tttatcaggt gccgtattca tgggattggg
    26941 ttattggtat gctacgccga aagcgaatcg tccggttgcg gacaaattta atcaaactga
    27001 aacggctgcc ggttaattac taaggggttt ttacgtggat catttgccta tattttgcca
    27061 attacgcgat cgcgactgtc tgattgtcgg cggtggtgat gtcgcggaac gcaaagcaag
    27121 gttgctgtta gacgcaggcg ctcgcttaac ggtgaatgca ttagcgttta ttccacagtt
    27181 caccgcatgg gcagatgcag gcatgttaac cctcgtcgaa gggccatttg atgaaagcct
    27241 tctcgacacc tgctggctgg cgattgcagc gacggatgat gacgcgctta accagcgcgt
    27301 cagcgaagcc gctgaagctc gtcgcatctt ctgtaacgtg gtcgatgcgc cgaaagctgc
    27361 cagcttcatt atgccgtcga ttattgaccg ctcaccgctg atggtggcgg tttcctctgg
    27421 cggtacttct ccggttctgg cgcgcctgtt gcgcgaaaaa ctcgaatcac tgctgccgct
    27481 gcatctgggc caggtagcga aatacgccgg gcagttacgc ggtcgggtaa aacaacagtt
    27541 cgccacgatg ggtgaacgcc gccgcttctg ggaaaaactg ttcgttaacg accgtctggc
    27601 gcagtcgctg gcgaacaacg atcagaaagc cattactgaa accaccgaac agttaatcaa
    27661 cgaaccgctc gaccatcgcg gtgaagtggt gctggttggt gcaggtccgg gcgatgccgg
    27721 gctgctgaca ctgaaaggtc tgcaacaaat tcagcaggca gatgtggtgg tctacgaccg
    27781 tctggtttct gacgatatta tgaatctggt acgccgcgat gcggatcgtg ttttcgtcgg
    27841 caaacgcgcg ggataccact gcgtaccgca ggaggagatt aaccagatcc tgctgcggga
    27901 agcgcaaaaa ggcaaacgcg tggtgcggct gaaaggcggc gatccgttta tttttggccg
    27961 tggtggcgaa gagctggaaa cactgtgcaa cgcgggtatt ccgttctcgg tggttccggg
    28021 tattaccgca gcttctggtt gctcagccta ttcgggtatt ccgctcacgc atcgcgatta
    28081 tgcccagagc gtgcgcttaa ttaccggaca cttaaaaacc ggtggcgagc tggactggga
    28141 aaacctggcg gcagaaaaac agacgctggt gttctatatg gggctgaatc aggctgcgac
    28201 tattcagcaa aagctgattg aacacggtat gcctggcgaa atgccggtag caattgtcga
    28261 aaacggaaca gcagtcacgc agcgcgtgat tgacggtacg ctcacacaac tgggcgaact
    28321 ggcgcagcaa atgaatagtc catcgctaat tattattggt cgggttgttg gcctgcgcga
    28381 taaactgaac tggttctcca accattaatt taatcagggc cagagaattc tggcctt
//
LOCUS       DBFIFI010000043        28247 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.043, whole genome shotgun sequence.
ACCESSION   DBFIFI010000043 DBFIFI010000000
VERSION     DBFIFI010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28247
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.043"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..519
                     /locus_tag="N7B39_001774"
     CDS             <1..519
                     /locus_tag="N7B39_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2421770.1"
                     /translation="IICITIKKRSSRSFIRGTCWLMGLFLIYSSYTVWERHSIWDYTF
                     PEPGITVTVPSKQWVANIVKQGPTLVTRDAHAILAIVAFSQNELGVHSIEELTATQNG
                     TAEMHVCDVQGFACAYQEGVQTMSDGKVRHAIFMTLLDNTNLIQLVAAIDPDYLDEYQ
                     EEVMKMMLSARQ"
     gene            642..1268
                     /locus_tag="N7B39_001775"
     CDS             642..1268
                     /locus_tag="N7B39_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2421771.1"
                     /translation="MVGEYIGSVLLGPLLLPVLVSGILCTFTKKTRNFASFTRGCCWV
                     LGVLLLSNVGNTFRLFTPWHYTFEKAAISVTVPNRHWNTVSISTDKTIDIRSEDNSVF
                     ISAFRLPAGRSADDSLEELKKMQRDNLKDQYNEETFQFHDCNAKHFTCKYQDVLINFD
                     GQQKRTISVYLEDTPRAVGIIALMEPDTADKYRQQAMEIMLSAKNTVK"
     gene            complement(1307..1552)
                     /gene="zapB"
                     /locus_tag="N7B39_001776"
     CDS             complement(1307..1552)
                     /gene="zapB"
                     /locus_tag="N7B39_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HGU2421772.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            1978..2823
                     /gene="glpF"
                     /locus_tag="N7B39_001777"
     CDS             1978..2823
                     /gene="glpF"
                     /locus_tag="N7B39_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HGU2421773.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            2846..4354
                     /gene="glpK"
                     /locus_tag="N7B39_001778"
     CDS             2846..4354
                     /gene="glpK"
                     /locus_tag="N7B39_001778"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HGU2421774.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            4584..5594
                     /gene="glpX"
                     /locus_tag="N7B39_001779"
     CDS             4584..5594
                     /gene="glpX"
                     /locus_tag="N7B39_001779"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HGU2421775.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANSLA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIESGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            5691..6437
                     /gene="fpr"
                     /locus_tag="N7B39_001780"
     CDS             5691..6437
                     /gene="fpr"
                     /locus_tag="N7B39_001780"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HGU2421776.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVHPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(6442..6870)
                     /gene="uspD"
                     /locus_tag="N7B39_001781"
     CDS             complement(6442..6870)
                     /gene="uspD"
                     /locus_tag="N7B39_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HGU2421777.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKLSADLLIVPFIDK"
     gene            complement(6897..7196)
                     /gene="yiiS"
                     /locus_tag="N7B39_001782"
     CDS             complement(6897..7196)
                     /gene="yiiS"
                     /locus_tag="N7B39_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HGU2421778.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(7408..7848)
                     /gene="yiiR"
                     /locus_tag="N7B39_001783"
     CDS             complement(7408..7848)
                     /gene="yiiR"
                     /locus_tag="N7B39_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HGU2421779.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            7949..8548
                     /gene="yiiQ"
                     /locus_tag="N7B39_001784"
     CDS             7949..8548
                     /gene="yiiQ"
                     /locus_tag="N7B39_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HGU2421780.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            8656..9423
                     /gene="tpiA"
                     /locus_tag="N7B39_001785"
     CDS             8656..9423
                     /gene="tpiA"
                     /locus_tag="N7B39_001785"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HGU2421781.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(9478..10233)
                     /gene="cdh"
                     /locus_tag="N7B39_001786"
     CDS             complement(9478..10233)
                     /gene="cdh"
                     /locus_tag="N7B39_001786"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HGU2421782.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(10340..11329)
                     /gene="sbp"
                     /locus_tag="N7B39_001787"
     CDS             complement(10340..11329)
                     /gene="sbp"
                     /locus_tag="N7B39_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HGU2421783.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKLGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLISWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(11648..12610)
                     /gene="pfkA"
                     /locus_tag="N7B39_001788"
     CDS             complement(11648..12610)
                     /gene="pfkA"
                     /locus_tag="N7B39_001788"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HGU2421784.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(12791..13693)
                     /gene="fieF"
                     /locus_tag="N7B39_001789"
     CDS             complement(12791..13693)
                     /gene="fieF"
                     /locus_tag="N7B39_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HGU2421785.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(13842..14342)
                     /gene="cpxP"
                     /locus_tag="N7B39_001790"
     CDS             complement(13842..14342)
                     /gene="cpxP"
                     /locus_tag="N7B39_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HGU2421786.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            14492..15190
                     /gene="cpxR"
                     /locus_tag="N7B39_001791"
     CDS             14492..15190
                     /gene="cpxR"
                     /locus_tag="N7B39_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HGU2421787.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            15187..16560
                     /gene="cpxA"
                     /locus_tag="N7B39_001792"
     CDS             15187..16560
                     /gene="cpxA"
                     /locus_tag="N7B39_001792"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HGU2421788.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(16666..17340)
                     /gene="yiiM"
                     /locus_tag="N7B39_001793"
     CDS             complement(16666..17340)
                     /gene="yiiM"
                     /locus_tag="N7B39_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HGU2421789.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYIGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(17489..18472)
                     /gene="kdgT"
                     /locus_tag="N7B39_001794"
     CDS             complement(17489..18472)
                     /gene="kdgT"
                     /locus_tag="N7B39_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HGU2421790.1"
                     /translation="MQIKRLIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(18732..19352)
                     /gene="sodA"
                     /locus_tag="N7B39_001795"
     CDS             complement(18732..19352)
                     /gene="sodA"
                     /locus_tag="N7B39_001795"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HGU2421791.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            19637..20671
                     /gene="rhaT"
                     /locus_tag="N7B39_001796"
                     /pseudo
     CDS             19637..20671
                     /gene="rhaT"
                     /locus_tag="N7B39_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(20668..21516)
                     /gene="rhaR"
                     /locus_tag="N7B39_001797"
     CDS             complement(20668..21516)
                     /gene="rhaR"
                     /locus_tag="N7B39_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HGU2421792.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(21590..22426)
                     /gene="rhaS"
                     /locus_tag="N7B39_001798"
     CDS             complement(21590..22426)
                     /gene="rhaS"
                     /locus_tag="N7B39_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HGU2421793.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYHCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            22714..24183
                     /gene="rhaB"
                     /locus_tag="N7B39_001799"
     CDS             22714..24183
                     /gene="rhaB"
                     /locus_tag="N7B39_001799"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HGU2421794.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALISATQALPACRFIINP
                     NDDRFINPETMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVARIHSTRQTKELCA"
     gene            24180..25439
                     /gene="rhaA"
                     /locus_tag="N7B39_001800"
     CDS             24180..25439
                     /gene="rhaA"
                     /locus_tag="N7B39_001800"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HGU2421795.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWIIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKETLSRRG"
     gene            25614..26438
                     /gene="rhaD"
                     /locus_tag="N7B39_001801"
     CDS             25614..26438
                     /gene="rhaD"
                     /locus_tag="N7B39_001801"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HGU2421796.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAL
                     YHDNFHPQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDETFGLIDTAEKSAEVLVKVYSMGGMKQTISREELIALGQRFGVTPLASALAL"
     gene            26448..26762
                     /gene="rhaM"
                     /locus_tag="N7B39_001802"
     CDS             26448..26762
                     /gene="rhaM"
                     /locus_tag="N7B39_001802"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HGU2421797.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTEICQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            complement(26803..28197)
                     /locus_tag="N7B39_001803"
     CDS             complement(26803..28197)
                     /locus_tag="N7B39_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HGU2421798.1"
                     /translation="MKKSTLSLAIGLLLACSTGMAKTQHLMLEQRMALLEERLEAAEM
                     RAAKAESQVKQLQTQQAAEIREIKAAQGNTPVNGQATAESAKKNATSPNLLLSGYGDL
                     KIYGDVEFNMDAESNHGLLAMTNADVNSDPTNEQWNLNGRILLGFDGMRKMDNGYFAG
                     FSAQPLGDMHGSVNIDDAVFFFGKENDWKVKVGRFEAYDMFPLNQDTFVEHSGNTAND
                     LYDDGSGYIYMMKEGRGRSNAGGNFLVSKQLDNWYFELNTLLEDGTSLYNDGNYHGRD
                     MEQQKNVAYLRPVIAWSPTEEFTVSAAMEANVVNNAYGYTDSKGNFVDQSDRTGYGMS
                     MTWNGLKTDPDNGIVVNLNTAYLDANNEKDFTAGINALWKRFELGYIYAHNKIDEFSG
                     VVCDNDCWIDDEGTYTIHTIHASYQFANVMDMENFNIYLGTYYSILDSDGDKKHGDDT
                     DDRYGARVRFKYFF"
BASE COUNT         7020 a         7245 c         7071 g         6911 t
ORIGIN      
        1 atcatctgta taacgataaa aaaaagaagc agccgcagct ttattcgcgg cacttgttgg
       61 ttaatgggat tatttcttat ctatagcagc tatacggtgt gggaacgaca cagcatatgg
      121 gattatactt tcccggagcc aggtatcacc gtcacggttc cgagcaaaca gtgggttgcc
      181 aatatagtaa aacaaggacc aaccctggtg acccgggatg cccatgctat tttagctatt
      241 gttgccttct cacaaaatga actcggtgtg cattcaatcg aggaacttac cgcgactcaa
      301 aacggaactg cagaaatgca tgtttgtgat gttcagggat ttgcctgcgc ttatcaggaa
      361 ggagtgcaaa caatgagtga tggcaaagta agacatgcta ttttcatgac tctactggat
      421 aacactaatt tgatacaact ggtcgcagct atcgacccag attatttgga cgaatatcaa
      481 gaagaagtaa tgaaaatgat gctttctgct cgtcaatgat ctgatgtgca tgttaataat
      541 aatggattga tatgagatac gttatcttat taatactatg gataataact gtttttctta
      601 gtcatcttca gcacagtaac ttgcttactg gagatccagg gatggttggc gagtatatcg
      661 gttcagtact tcttggaccg ttattacttc ccgtgcttgt gtcaggtatt ctgtgcacat
      721 tcactaaaaa aacgcgtaac ttcgccagtt ttactcgcgg gtgctgttgg gtattgggag
      781 tcctgctttt atccaacgtc ggcaatacgt ttcgtctgtt caccccgtgg cactatacgt
      841 ttgagaaggc agctatttcc gtgactgtcc caaaccgaca ctggaatacg gtttctatta
      901 gtactgataa aaccattgat ataagaagtg aagataatag cgtctttatt tctgctttcc
      961 gcctgcccgc aggacgtagc gccgatgact cgctggaaga gttaaagaag atgcagcgag
     1021 acaatctcaa ggatcaatac aatgaagaaa ccttccagtt tcatgattgt aatgccaaac
     1081 atttcacctg taagtatcag gatgtgttga taaattttga tggtcagcag aaaagaacca
     1141 tcagtgttta tcttgaagac acccccaggg ctgtggggat cattgcatta atggagccag
     1201 atacggcaga taaatatcgc caacaggcga tggagattat gttgtctgct aaaaatacag
     1261 tgaagtaaaa aataaggcgc cttgcggcgc ctctgcgtgg aagagattaa acctcttcca
     1321 tgcgacccag cagggcctgc agacgttcct gccagccgtt ctgctgttct ttcagatggt
     1381 tgttctcacg ctccagctct tcgcgctgat gctgggcatt ttgaacttcc tgcgacaatg
     1441 agttgttttt ttctttcagc tcttcgattt ccatctgcaa cagagtgatg gtatcaatcg
     1501 cctgctgtac ttttgcttcc agtttctcaa acacttctaa tgacattgtc atacctctcc
     1561 tgaattgcaa ggcgttgatg gataaaaaat cctcgtcccg attaccggtg acgccttaat
     1621 aaatacgagc gcactttagt tagctccgat tgtatgaagc cgcgccatcg ctgtccagcg
     1681 gcacgccttg cagattacgg tttgccacac ttttcatcct tctcctggtg acataatcca
     1741 caccaatcga aaatgttaat aaatttgttg cgcgaatgtt ctaacaaaca tgcatcatgt
     1801 acaatcagat ggaataaatg gcgcgataac gctcatttta tgacgaagca cacacatttt
     1861 aagttcgata tttctcgttt ttgctcgtta acgataagtt tacagcatgc ctacaagcat
     1921 cgtggaggtc cgtgactttc acgcatacaa caaacattaa ctcttcagga tccgattatg
     1981 agtcaaacat caaccttgaa aggccagtgc attgctgaat tcctcggtac cgggttgttg
     2041 atttttttcg gtgtgggttg cgttgcagca ctaaaagtcg ctggtgcgtc ttttggtcag
     2101 tgggaaatca gtgtcatttg gggactgggg gtggcaatgg ccatctacct gaccgcaggg
     2161 gtttccggcg cgcatcttaa tcccgctgtt accattgcat tgtggctgtt tgcctgtttc
     2221 gacaagcgca aagttattcc ttttatcgtt tcacaagttg ccggcgcttt ctgtgctgcg
     2281 gctttagttt acgggcttta ctacaattta tttttcgact tcgagcagac tcatcacatt
     2341 gttcgcggca gcgttgaaag tgttgatctg gcaggcactt tctctactta ccctaatcct
     2401 catatcaatt ttgtgcaggc tttcgcagtt gagatggtga ttaccgctat tctgatgggg
     2461 ctgatcctgg cgttaacgga cgatggcaac ggtgtaccac gcggcccttt ggctcccttg
     2521 ctgattggtc tactgattgc ggtcattggc gcatctatgg gcccattgac gggttttgcc
     2581 atgaacccag cgcgtgactt cggtccgaaa gtctttgcct ggctggcggg ctggggcaat
     2641 gtcgccttta ccggcggcag agacattcct tacttcctgg tgccgctttt tggccctatc
     2701 gttggcgcga ttgtaggtgc atttgcctac cgcaaactga ttggtcgcca tttgccttgc
     2761 gatatctgtg ttgtggaaga aaaggagacc acaactcctt cagaacaaaa agcttcgctg
     2821 taatatgact acgggacaat taaacatgac tgaaaaaaaa tatatcgttg cgctcgacca
     2881 gggcaccacc agctcccgcg cggtcgtaat ggatcacgat gccaatatca ttagcgtgtc
     2941 gcagcgcgaa tttgagcaaa tctacccaaa accaggctgg gtagaacacg acccaatgga
     3001 aatctgggcc acccaaagct ccacgctggt agaagtgctg gcgaaagccg atatcagttc
     3061 cgatcaaatt gcagctatcg gtattacgaa ccagcgtgaa accactattg tctgggaaaa
     3121 agaaaccggc aagcctatct ataacgccat tgtctggcag tgccgtcgta ccgcagaaat
     3181 ctgcgagcat ttaaaacgtg acggtttaga agattatatc cgcagcaata ccggtctggt
     3241 gattgacccg tacttttctg gcaccaaagt gaagtggatc ctcgaccatg tggaaggctc
     3301 tcgcgagcgt gcgcgtcgtg gtgaattgct gtttggtacg gttgatacgt ggcttatctg
     3361 gaaaatgact cagggccgtg tccatgtgac cgattacacc aacgcctctc gtaccatgtt
     3421 gttcaacatc cataccctgg actgggacga caaaatgctg gaagtgctgg atattccgcg
     3481 cgagatgctg ccagaagtgc gtcgttcttc cgaagtgtac ggtcagacta acattggcgg
     3541 caaaggcggc acgcgtattc caatctccgg gatcgccggt gaccagcagg ccgcgctgtt
     3601 tggtcagttg tgcgtgaaag aagggatggc gaagaacacc tatggcactg gctgctttat
     3661 gctgatgaac actggcgaga aagcggtgaa atcagaaaac ggcctactga ccaccatcgc
     3721 ctgcggcccg actggcgaag tgaactatgc gttggaaggt gcggtgttta tggcaggcgc
     3781 atccattcag tggctgcgcg atgagatgaa gttgattaac gacgcctacg attccgaata
     3841 tttcgccacc aaagtgcaaa acaccaatgg tgtgtatgtg gttccggcat ttaccgggct
     3901 gggtgcgccg tactgggacc cgtatgcgcg cggggcgatt ttcggtctga ctcgtggggt
     3961 gaacgctaac cacattatac gcgcgacgct ggagtctatt gcttatcaga cgcgtgacgt
     4021 gctggaagcg atgcaggccg actctggtat ccgtctgcac gccctgcgcg tggatggtgg
     4081 cgcagtagca aacaatttcc tgatgcagtt ccagtctgat attctcggca cccgcgttga
     4141 gcgcccggaa gttcgcgaag tcaccgcatt gggtgcggcc tatctcgcag gcctggcggt
     4201 tggcttctgg cagaacctcg acgagctgca agagaaagcg gtgattgagc gcgagttccg
     4261 tccaggcatc gaaaccactg agcgtaatta ccgttacgca ggctggaaaa aagcggtcaa
     4321 acgcgcgatg gcgtgggaag aacacgacga gtaatgtcag gcacctgaat cgattgcctg
     4381 atgctgatcg cctgatgcga cgcttacgcg tcttatcagg cctacgagcc aatgccattt
     4441 tgtgtaggcg ggataaggcg ttcacgccgc atccggcatc acgcagactc tgaaaccgtg
     4501 cgggggcaac cccgcacaca tcaataatcc cacccttccc ctgtgctaca cttcgcgcca
     4561 ttccttactg cttagagttt gctatgagac gagaacttgc catcgaattt tcccgcgtca
     4621 ccgaatctgc ggcgctggct ggctacaaat ggttaggacg cggcgataaa aacaccgcgg
     4681 acggcgcggc ggtaaacgcc atgcgtatta tgctcaacca ggtcaacatt gacggcacca
     4741 tcgtcattgg tgaaggtgaa atcgacgaag caccgatgct ctacattggt gaaaaagtcg
     4801 gtactggtcg cggcgacgcg gtagatattg ctgttgatcc gattgaaggc acgcgcatga
     4861 cggcgatggg tcaggctaac tcgctggcgg tgctggcagt aggcgataaa ggctgtttcc
     4921 tcaacgcgcc agatatgtat atggagaagc tgatcgtcgg accgggagcc aaaggcacca
     4981 ttgatctgaa cctgccgctg gcggataacc tgcgcaatgt cgcggcggcg ctcggcaaac
     5041 cgttgagcga actgacggta acgattctgg ctaaaccacg ccacgatgcc gttatcgctg
     5101 aaatgcagca actcggcgta cgcgtatttg ctattccgga tggcgacgtt gcggcctcaa
     5161 ttctcacctg tatgccagac agcgaagttg acgtgctgta cggaattggt ggcgcgccgg
     5221 aaggcgtggt ttctgcggcg gtgatccgcg cattagatgg cgacatgaac ggtcgtctgc
     5281 tggcgcgtca tgacgtcaaa ggcgacaacg aagagaatcg tcgcattggc gagcaggagc
     5341 tggcacgctg caaagcgatg ggcatcgaat ccggtaaagt attgcgtctg ggcgatatgg
     5401 cgcgcagcga taacgtcatc ttctctgcca ccggtattac caaaggcgat ctgctggaag
     5461 gcattagccg caaaggcaat atcgcgacta ccgaaacgct gctgatccgc ggcaaatcac
     5521 gcaccattcg ccgcattcag tctatccact atctggatcg caaagacccg gaaatgcagg
     5581 tgcacatcct ctgattgatt tgatcgattg agccttccag tccttcggga ctggaatttt
     5641 tttgttcgga gaacgaagat aaggcaattc aatcaaaaca ggagaaaaac atggctgatt
     5701 gggtaacagg caaagtcact aaagtgcaga actggaccga cgccctgttt agtctcaccg
     5761 ttcacgcccc tgtgcatccg tttaccgccg ggcaatttac caagcttggc cttgaaatcg
     5821 acggcgaacg cgtccaacgc gcctactcct atgttaactc gcccgataat cccgatctgg
     5881 agttttacct ggtcaccgtc cccgatggca aattaagccc acgattggcg gcactgaaac
     5941 cgggcgatga agtgcaggtg gttagcgaag cagctggctt ctttgttctg gatgaagtac
     6001 cagattgcga aacgctatgg atgctggcaa ccggtacagc gattggccct tatttatcga
     6061 ttctgcaact aggtaaagat ttagatcgct tcaaaaatct ggtcctggtt cacgccgcac
     6121 gttatgccgc cgacttaagc tatttgccac tgatgcaaga actggaaaaa cgctacgaag
     6181 gcaaactgcg cattcagacg gtggtcagtc gggaaacggc agcggggtcg ctcaccggac
     6241 ggataccggc attaattgaa agtggtgaac tggaaagcgc gattggcctg ccgatgaata
     6301 aagaaaccag ccatgtgatg ctgtgcggca atccacagat ggtgcgcgat acgcaacagt
     6361 tgctgaaaga gacccggcag atgacgaaac atttacgtcg ccgaccgggt cacatgacag
     6421 cggagcatta ctggtaagcg gttacttatc gataaacggc acgatgagca aatccgcact
     6481 caacttattg atcatcccgc gatatgccgg catcaaacgg ttgataaatg agtggtgatg
     6541 accacagaca aggaggtcgc actgctcttt ttgcataatt tccagcagtg tttccggcat
     6601 ttctccgcgt tcaatacgca gttttgtctt cggccattga atatttttcg tcagtttata
     6661 cagcttgtta tccgacttat tcttcaacaa ttgaagaata tcttctgttg cagggaagta
     6721 gatacccgga tacaactcgc ttaagccatc atcaatatga attaacgtca ggtgagcgtc
     6781 attatgtctg gcgagctcca aggctttatt caccagtaag gcatcttctt cattcccgga
     6841 aattgccacg ccaatgtgtt tataagccat gtttaactcc ttctaaagca actccatcaa
     6901 gctaacgaac gcagtgataa ctcaaaaata atcatctcgg cctggcatga gaaggtgaag
     6961 gccgcatcaa gctcaacttg cccctccaga tccttaatct tataggccac gctggcccct
     7021 tcatctgcag aggatgcagc ggcggcaagc tctttcagtt tggtcataaa ggactctgct
     7081 tcttcgcggg tggcaaaaaa gcgcgaaaac ttactggtac agttgtcgtt atcaatcacc
     7141 gtaccgatat caatcgcaca tcctttagta ctgcatttat ctacgacatc tttcataggg
     7201 cacctctgta tattcgcctc tctgtcgatt acgacttaag aataatgcgc cgttaaggga
     7261 agaacaaaca agtaatgatc ttatggatta ccattttttt tgatattgcg taaggtgaag
     7321 agttcagaag gattttccgg gtattgtcat ttgcatcgtg gaaataacaa tcccggaaaa
     7381 aaatagcagt aagagatata ttaaaggtta attacttgat ttattgtcgg ctttatactt
     7441 cacatcctga gtatctttac catatttatt ttcgccttgt gtgccaacaa acgcaccaag
     7501 atcgataagc atcatcacca gaatcaacgt cgggacaaaa cgccctaccg cccattgcca
     7561 gacacccggt aaaatcgccc agttacccgc cagtagcatc cacgccacaa tcatcagaaa
     7621 tgcccatgcg ccggaacgcc cgcgatcatg caagcgctta acggttactg ccgctgttgg
     7681 ccagagcaag cacacaaggc aaaacgccgc ggtctgaata tcgagtaaat ttttacccgc
     7741 cagtgagaaa agcaccagca tgcctgcgaa ccacaggcct atccaaatcc agaaatcacg
     7801 gcgtccaata cgccctttaa atgagaataa ccattgctgt atggtcatgt aagttccttg
     7861 atggttgtct tttccaggat tctacccttt tgacaagggc gacagatatc gttttaatcg
     7921 gagccagtca taacaaaagg tactgtcaat gaagccaggg tgtacgctgt tttttctctt
     7981 atgttctgca ttaaccgtta caacaacggc gcatgcacaa acaccagata cggcaacgac
     8041 cgcgccttat ctgctggctg gagcccctac tttcgatctc tccatcagcc agtttcgaga
     8101 agactttaac agccagaatc ccagcctgcc actgaacgaa tttcgtgcca tcgacagcag
     8161 tcccgacaaa gccaatctca ctcgtgctgc cagtaaaatt aacgagaact tgtatgcttc
     8221 tacagcgctg gagcgcggta ccttaaaaat caaaagcatt caaatgacat ggctacccat
     8281 ccaggggcca gagcaaaaag ccgcgaaagc gaaagctcag gaatacatgg cagcggtgat
     8341 ccgcacactc accccattaa tgaccaaaac acaaagccag aaaaaactgc agtcgctact
     8401 aacggcgggg aaaaacaaac gttattacac cgagacagaa ggtgcactgc gctatgttgt
     8461 cgcggacaac ggcgaaaagg ggctgacctt cgctgttgaa ccgattaagc tggcgctatc
     8521 tgaatcgctt gaaggtttga ataaatgaca aaaagcaaag cctttgtgcc gatgaatctc
     8581 tatactgttt cacagacctg ctgccctgcg gggcggccat cttcctttat tcgcttataa
     8641 gcgtggagaa ttaaaatgcg acatccttta gtgatgggta actggaaact gaacggcagc
     8701 cgccacatgg ttcacgagct ggtttctaac ctgcgtaaag agctggcagg tgttgctggc
     8761 tgtgcggttg caatcgcacc accggaaatg tacatcgata tggcgaagcg tgaagctgaa
     8821 ggcagccaca tcatgctggg tgcgcaaaac gtggacctga acctgtccgg cgcattcacc
     8881 ggtgaaacct ctgctgctat gctgaaagac atcggcgcac agtacatcat catcggtcac
     8941 tctgaacgtc gtacttacca caaagaatct gacgaactga tcgcgaaaaa attcgcggtg
     9001 ctgaaagagc agggcctgac tccggttctg tgcatcggtg aaaccgaagc tgaaaacgaa
     9061 gcgggcaaaa ctgaagaagt ttgcgcacgt cagatcgacg cggtactgaa aactcagggt
     9121 gctgcggcat tcgaaggtgc ggttatcgct tacgaacctg tatgggcaat cggtactggc
     9181 aaatctgcaa ctccggctca ggcacaggct gttcacaaat tcattcgtga ccacatcgct
     9241 aaagttgacg ctaacatcgc tgaacaagtg atcattcagt acggcggctc tgtaaacgcg
     9301 tctaacgctg cagaactgtt tgctcagcca gacatcgacg gcgcgctggt tggcggtgcg
     9361 tctctgaaag ctgacgcttt cgcagtaatc gttaaagctg cagaagcggc taaacaggct
     9421 taagtctgac aggtgccgga tttcatatcc ggcacttact ttccttaact cttcgcctta
     9481 acgcaaaatc tcacactgat gatcctgaat ttcctcggct gaagcacggt taagcgtcag
     9541 tagatttcgt tgtgtcgcca gcaatacaaa tgagttatca ctctgccgta ccatcgccag
     9601 cccgtagctt cccatatgtt cccgcgcctc aggtacttct tctgccagca tcataaatgg
     9661 gctgcgttgt accagttcgc tttccgttac ccgacgcgcc aggtattcat gcccgcgcaa
     9721 accacctggc agtggcaacc agcggctgct gatgttcgcc agattgttat ccagctgttc
     9781 gcgcacatca ggacgaatac aagagatatg aatatgaaaa tggttttgcg tacgcccggt
     9841 gcgggagttg atcgccaaag aaaccgcgcg atcgggaacc ggctggccgt attttttgct
     9901 cataaaatca cgcgcctgcc aggccaacca aaagaagttc ggcgttgaag gatcggtcaa
     9961 caaaggactt tcagtaccgt taatacgata cgttggcatc aacagatatt gcagtgggcc
    10021 attaagatct tttaaaacca cgtatccggc attgggtttg acttccgcac atggcgaagg
    10081 attttgattt tgctgctgat tgggcaaaca ttcctcaagg acaatcttac gtaatgtatc
    10141 cgactcttca ccggttaatt tccagtaacc aataccggca gccacaacgg cgataactat
    10201 catcaccaaa aaaagaagac ccgctttttt catctttttt tccctgtacc tcaaagagat
    10261 gcaagggtaa cgcaaaatcg tgacaaataa aaaacccggt cgcgagaaac caccgggttt
    10321 gataattatc ctggggaaat cagcgtttgc tgatctgatc gaacgtaccg ccgttagcaa
    10381 aatgctcttt ttgcgctttc gtccagccgc cgaactcttc atcaatggtg aataacttca
    10441 gctttggaaa cgcattttcg tactttttcg ccacctcagc gtcgcgcgga cggtagtagt
    10501 ttttcgcggc aatttcctga ccttctggcg agtagagata tttcaggtag gcttccgcca
    10561 cctctttagt accttttttc tcgaccactt tatcgaccac cgacacggtt ggctctgcga
    10621 ggatagactc actcggcgtg acgatttcga atttatcttt ccccagttca ttcgctgcca
    10681 gcagagcttc gttttcccag gaaatcagta catcgccaat tccgcgctcg acaaaagtgt
    10741 tagtggagcc gcgcgcgcca gaatccagaa cttcgacgtt tttatacagt gcccgaacaa
    10801 aatcctgtgc ttttgcctga tcgttgttgt tgtgatgcag cgcgtagccc caggctgcca
    10861 ggtagttcca gcgcgcgcca ccagagcttt tcggattagg cgtgatcacc gaaacaccca
    10921 gtttaatcag atcgttccag tcatggatct gcttcggatt tcccttacgt accaggaaaa
    10981 caatggtgga agtgtacggt gcggagttat ccggcagacg tttgatccac tctttatcaa
    11041 tccgcccgcg ttccgcaatc gcgtccacgt cataggccag agccagcgtg acaacatcag
    11101 cttcaatacc gttgattacc gacgttgctt gtttgcctga accaccgtgt gactgacgaa
    11161 tcaccacgtt atcgccagtt tgctgtttcc agtgggcgct gaatgccttg ttgtactgtt
    11221 cgtacaattc gcgcgttgga tcatatgaaa cgttaagaag ctgaatatcc tttgccataa
    11281 cgctggttgc cgccagcaaa aatgttaacc ctacgcccca cttgttcatc gcccgactct
    11341 cttatgttgt gttgtgatga gcaaagcgtg ccagaaggtt aaccaaacat taaagaataa
    11401 aaaaagattg gctataactt gcgggtatat gttgggggtt aaaaaggcgg aagaaatccg
    11461 cctcatattg ctgacaaagt gcgctttatc gatgccggat gcggcgcgaa cgccttatcc
    11521 ggcctacaaa agtttgtaaa ttcaataaat tgtagaattc acgtaggcct gataagcgaa
    11581 gcgcatcagg catttttgct tctgtcatcg gtttcaggct aaaggaatct gcctttttcc
    11641 gaaatcatta gtacagtttt ttcgcgcagt ccagccagtc gcctttgaac ggacgcttca
    11701 tgttttcgat agcgtcgatg atgtcgtggt gaaccagctg ttcgttctgt atacctacgc
    11761 aacgaccgcc gtaacctgcc agcagcagat cgatagcgta agcgcccata cgggaagcca
    11821 gaatacggtc gtaaggcacc ggagaaccac cgcgctggat gtggcccagc acagttgcgc
    11881 gggtttcacg accggtttct ttctcgatga aatgcgccag ttcgtcaaca tcacacatat
    11941 gttcggtaat cgccacgatc gcgtgttttt tacctttcgc gatacccgct ttgatttcgt
    12001 ttaccaggtc ttcacggctg aattcaactt ccggaaccac aacgaattca cagccaccgg
    12061 caatggccgc agccagcgtc agatcgccac aataacggcc catcacttcc accacggaaa
    12121 tacgctggtg agaagaagag gtgtcacgca gacggtcgat cgcttctaca acggtgctca
    12181 gcgcagtgaa gaaaccgata gtgtagtcag tgcctttgat gtcgttgtcg atagtgcccg
    12241 gcaggccgat gcacgggaag cccatttcgg tcagacgcat tgcacccatg taggaaccgt
    12301 caccgccgat aaccaccagc gcatcgatcc cacgtttttt caggttttcg atagccacgg
    12361 cgcggatgtt ctcgtcgcgg aattccggga aacgcgcaga accgaggaac gtaccgccac
    12421 ggttgatcat gtcagaaacg ctgtaacggt ctagctgtac catacggtct tcatacagac
    12481 ccagatagcc gtcataaatg cccattactt ccagaccttc tgtcagcgca gaacgaacaa
    12541 ccccgcgaat tgcggcgttc atgcctggcg catcaccgcc gcttgtcaac acaccgattt
    12601 tcttaatcat gactacctct gaactttgga atgcaaaata aaatctgttg ccggaagtct
    12661 tcttgcacat cgaagtgatc caacgaatgt gcaaatagta taacaatcac ttcctgctga
    12721 attgattcag gtcaggccaa atggcggtat tttatacaca aaatgcgggt ctggctctct
    12781 tttatactga ttatgaaagc atagaccgtt taccctccct aggtacgacg gaacaggggt
    12841 cctgatggat aatcacatcc gatcccggaa aacgccgtaa aatagcctgc tctacctgat
    12901 ccgccaccat atgtgcctga accaaaggca gagagtcttc catttccaaa tgaatctgaa
    12961 taaagcgggt cggccctgac tgccgcgtgc gaagatcgtg agcgccgcta acacccggcc
    13021 aggaagtcac gatatcaata atttcttgcc gttcctcatc aggtaatgcg cgatccagta
    13081 atgactgtac cgcctcatat cccatgcgta acgcgctata taaaatatag atgccgattc
    13141 ccaatgcaaa cagagcatcg gcgcgatgcc agccgtacca ggacaacccc agcgccagca
    13201 gaattgcgcc gttcatcata acatcagact ggtaatgtag catatcagcc cgcaccgcct
    13261 ggctttgcgt ccggcgcacc acccaacgct gaaacgagac aaggataatc gtacaaatta
    13321 gcgccacaat tgtcacgata accccgacgc ctggatctgt catcggtgtt ggagatatca
    13381 gatgttgaat gcctgtcaaa aacaggaata gtgccgaacc ggagataaac atactttgcg
    13441 ccagcgccgc gagggactca gctttaccgt gaccaaacga gtgattatcg tcggcaggtt
    13501 gcagggaata tcgcaccacc aataaattcg tcaacgacgc gccgatatcc accagcgaat
    13561 ccaccagcgc ggcgagaata ctcaccgacc cggtatacca ccatgcaaaa attttaatca
    13621 gcaatagcag tgaagccatc gccgtcgcag caatcgccgc ccgactgacc agccgtccat
    13681 aagattgatt cataaatact cccgctatca actgacgcta gtataacgga agcaaatcat
    13741 ctgcaatgca ttaagcagca ggcaaattga ggataaaaaa aacccccaca tcatgtgggg
    13801 gaagacaggg atggtgtcta tggcaaggaa aacagggttt actactggga acgtgagttg
    13861 ctactactca atagcttcaa cgatgaactt ttttgccatt gcgtcacgtc acgcaactgc
    13921 tccattcgtt gttgatgttt ctcgtttaaa accgcttgct gctccggcgt taacaggcga
    13981 tacatttggt tgcggacttt tgccatctca acctgacgag caatttgctc attcgccatt
    14041 ttttctgcct gtgcgcgcac agcgttttca tcaaaatttt ctgcggtgac aagacgatgc
    14101 attgtctcca gttcgctaac attaacagga ggctgttcgt gccgggcctg ttgcataaga
    14161 tctcgcatct gctgacgctg atgttcggtt aaacttatgc cgtcgaacat atggctctgc
    14221 gtactgcgct gcgtaagttc ttcacccgga tgccagttat cgcctgaacc gacttcagca
    14281 gcgtggctta atgaactgac tgccagcgtt gaggccatga cggcagcggt aactatgcgc
    14341 atcatttgct cccaaaatct ttctgtcgcg attcaacgat agagagttta cgattcaggc
    14401 tgcaaacatg cgtcaggggg tgtaaaacaa cgtaaagtca tggattagcg acgtctgatg
    14461 acgtaatttc tgcctcggag gtatttaaac aatgaataaa atcctgttag ttgatgatga
    14521 ccgagagctg acttccctat taaaggagct gctcgagatg gaaggcttca acgtgattgt
    14581 tgcccacgat ggggaacagg cgcttgatct tctggacgac agcattgatt tacttttgct
    14641 tgacgtaatg atgccgaaga aaaatggtat cgacacatta aaagcacttc gccagacaca
    14701 ccagacgcct gtcattatgt tgacggcgcg cggcagtgaa cttgatcgcg ttctcggcct
    14761 tgagctgggc gcagatgact atctcccgaa accgtttaat gatcgtgagc tggtggcacg
    14821 tattcgcgcg atcctgcgcc gttcgcactg gagcgagcaa cagcaaaaca acgacaacgg
    14881 ttcaccgaca ctggaagttg atgccttagt actgaatcca ggccgtcagg aagccagctt
    14941 cgacgggcaa acgctggagt taaccggtac tgagtttacc ctgctctatt tgctggcaca
    15001 gcatctgggt caggtggttt cccgtgaaca tttaagccag gaagtgctgg gcaaacgcct
    15061 gacgcctttc gaccgcgcta tcgatatgca catttccaac ctgcgtcgta aactgccgga
    15121 tcgtaaagat ggtcacccgt ggtttaaaac cttgcgtggt cgcggctacc tgatggtttc
    15181 tgcttcatga taggcagctt aaccgcgcgc atcttcgcca tcttctggct gacgctggcg
    15241 ctggtgttga tgttggtttt gatgttaccc aagctcgatt cacgccagat gaccgagctt
    15301 ctggatagcg aacagcgtca gggtctgatg attgagcagc atgttgaagc ggagctggcg
    15361 aacgatccgc ccaacgattt aatgtggtgg cggcgtctgt tccgggcgat tgataagtgg
    15421 gcaccgccag gacagcgttt gttattggtg accaccgaag gccgcgtgat cggcgctgaa
    15481 cgcagcgaaa tgcagatcat tcgtaacttt attggtcagg ccgataacgc cgatcatccg
    15541 cagaagaaaa agtatggccg cgtggaactg gtcggtccgt tctccgtgcg tgatggcgaa
    15601 gataattacc aactttatct gattcgtccg gccagcagtt ctcaatccga tttcattaac
    15661 ttactgtttg accgcccgct attactgctg attgtcacca tgttggtcag tacgccgctg
    15721 ctgttgtggt tggcctggag tctggcaaaa ccggcgcgta agctgaaaaa cgctgccgat
    15781 gaagttgccc agggaaactt acgccagcac ccggaactgg aagcggggcc acaggaattc
    15841 cttgccgcag gtgccagttt taaccagatg gtcaccgcgc tggagcgcat gatgacctct
    15901 cagcagcgtc tgctttctga tatctctcac gagctgcgca ccccgctgac gcgtctgcaa
    15961 ctgggtacgg cgttactgcg ccgtcgtagt ggtgaaagca aggaactgga gcgtattgaa
    16021 accgaagcgc aacgtctgga cagcatgatt aacgacctgt tggtgatgtc acgtaatcag
    16081 caaaaaaacg cgctggttag cgagaccatc aaagccaatc agttgtggag tgaagtgctg
    16141 gataacgcgg cgttcgaagc cgagcaaatg ggcaagtcgt tgacagttaa cttcccgcct
    16201 gggccgtggc cgctgtacgg caacccgaac gccctggaga gtgcgctgga aaacattgtt
    16261 cgtaatgccc tgcgttattc ccatacgaag attgaagtgg gctttgcggt agataaagac
    16321 ggtatcacca ttacggtgga cgacgatggt cctggcgtta gcccggaaga tcgcgaacag
    16381 attttccgtc cgttctatcg gaccgatgaa gcgcgcgatc gtgaatctgg cggtacaggt
    16441 ttgggactgg cgattgttga aaccgccatt cagcagcatc gtggctgggt gaaagcagaa
    16501 gacagcccgc tgggcggttt acggctggtg atttggttgc cgctgtataa gcggagttaa
    16561 actccgcatt tgtaggcagg ataaggcgtt tacgccgcat ccggcatttg agcaggatgc
    16621 ctgatgcgac gctgatagcg tcttatcagg cctacactcc gggggttatt ttccccacaa
    16681 ccggcgggaa aaatcttcga tcttgccgct cagtcggcgc ttttgcatcg ttcgcgtcca
    16741 gcttttcgat aacccggcag cggagagtaa acggtgatac tgatcgtcat caaacggcat
    16801 atgccatgcg atggcagcag cctcctgcac ggtcacatcg ctgacacggg aaaccaactc
    16861 cagcggcgcg tccgcagaca ctttccctgg cgctatcacg ctatacaacc atcccacttt
    16921 acccgtgttt tgcatcaact gcgctatatc gctgatatca aaatggtaat tgagtttata
    16981 gcaaggcgag cgcggctggc tgacctgaat taatgcctct ccccagcgga aaatatcgcc
    17041 aatataaaca ttactttccg tgagaccgtc ggttgagaga ttttcgccaa acgcaggcgc
    17101 aacaaacaac tccgcctgtt ccggaaattc ccgcgcccag tagagataat gctcacgagg
    17161 ataatgacac agcgctctgt ctggtccgcc gtgaaccttt ttctccgcct gctcgtcacc
    17221 ttccagcccc agctccgtta gcatcaactc accatcaacc tggattttgg caattgcgct
    17281 gggtttgcct tcgggataag cctgaatctt gcctgtgtat acatcaaccg gatatcgcat
    17341 ctgttccctc tccacgagaa atttccccat tagaccatat ttattttatg tcaggcgtgc
    17401 tgttttcgcg gtaagtatcc aggcaggaac tcaatagcaa accggacgaa cgttagttca
    17461 tccggctgtg gggattaagg aagcaggttt atttcaccgt acctaaaatt tcgattttcg
    17521 ccgctctggc tttgacttta cgtgaccaga tagaggtaag aattggcacc agaatcgaag
    17581 tcacaatgac tgccgtcgct accagcgaag ttgctgccgg agccatcggt ttaaacgcag
    17641 gcaccatttc tgcaatcagc acaggtgtcg ctaccgcggc ccctgcggaa ctggaagcgg
    17701 caattccggc agtgccatcg ccaccgccaa tcaatttatc ggcgataatc aacggaatac
    17761 cggtcacgat aattactgcc acacccaaca ggatccccag caaacctgtc tgggcaatta
    17821 cagtcaaatc aatggtattg cctagtgcga aggcaaagaa tggaatcagc gtctgcaccg
    17881 ctttgctgaa aaattctcgt aattcagggt caaggttccc aagggcaaag cccaccagga
    17941 acggtaatac tgcgccgacg aaaacatgcg gttcaaacga ggcaatcccg gcagtgccca
    18001 gaataatcat cgtcatgagc ggcccggact ccaacgacat caacacaaat gccccagctt
    18061 cttcttttgt gccgtactgc tgcatgatgg aagcgtaaag tccgccgttg gtcatatcca
    18121 tcgccgccac cagcgccagc gttgaaagtc cggcaaagaa tccaacttca acaccatgtt
    18181 ccggaataat gcgcgaggca attgccgcaa ccacccacgc gacggcaatt ttagttacca
    18241 ccagcgtacc ggatttacgc agtaccgttc ccgtcgcgct taattttatt gacgccccca
    18301 tgcaaaaaaa ccacaccgcc agaatgggca ccgtaccggt aatcatcccg ttggtgaatg
    18361 atccaaaata tttccccgcc cccggcgaga aggtgtggca cagtgcgcca aggaatagcg
    18421 ggacgagcat catccccccc gggattttct caatcaagcg ttttatctgc atttccatca
    18481 cctgtcggca tatttttaag ggctgatgcg taattcctca atggagcatc agtgttgatg
    18541 gaaacgaaga tagacattca tttaatcaat aaaagtgatc aaaaccacat caactgaaac
    18601 gctgttttat ttttatcgga tcattgtttt tattttgaaa ttgatcacaa aaaaaccacc
    18661 gccattggcg atggttcatt ttttaagctg atatgcggcg taaacgcctc actgcagcag
    18721 acggcaaatg attatttttt cgccgcgaaa cgtgccgctg cttcgtccca gttcaccacg
    18781 ttccagaact ctttgatgta gtccgggcgg cggttctgga atttcaggta gtaagcatgc
    18841 tcccacacat ccaggcccag aatcgggaag ccggatgcgc cagaaatagc ttcacccatc
    18901 agcggggagt cctggttagc agtagaaacc accgccagtt tatcgccttt cagcaccagc
    18961 catgcccagc cggaaccaaa gcgggaagct gccgctttct caaattctgc tttgaagtta
    19021 tctacggaac cgaaatcacg ttcgatagcc gctttcaggt caccctgcag ggtggtgcct
    19081 tttttcagac ctttccagaa caggctgtgg ttagcgtgac cgccagcgtt gttgcgcagt
    19141 acggttttct tgtctgctgg cagctggtcc agtttggtga tcaactcttc aaccggcagg
    19201 ttggcaaatt ctggcaggct ttccaacgcc gcgttggcgt tgttgacgta ggtctgatgg
    19261 tgtttggtgt ggtggatttc catggtctgc ttatcgaagt gcggttccag ggcatcgtaa
    19321 gcatacggca gggatggcag ggtatagctc atattcatct ccagtattgt cgggcggccg
    19381 attgttaatg ccgcgtaagc agttggttca ttatagttaa ttaaatgata ttgaaaatga
    19441 ttatcaatgc cgtacttttc gtaagggtat ggttttgcag gaaaatgccc gagatgtgaa
    19501 gcaaatcacc cacttaatgc cgtgattgcc agtaaatcga caacggcggc aacaggcgaa
    19561 aggttaatcg acagcacgat ttttacactc atctcgtcgg agatgtgacg cgacgaaaaa
    19621 tgatgaggat aagaagatga gtaacgcgat tacgatgggg atattttggc atttgatcgg
    19681 cgcggccagt gcagcctgtt tttacgctcc gttcaaaaaa gtaaaaaaat ggtcatggga
    19741 aaccatgtgg tcagtcggtg ggattgtttc gtggattatt ctgccgtagg ccatcagcgc
    19801 cctgttacta ccgaatttct gggcgtatta cagctcgttt agtctctcta cgctgctgcc
    19861 tgtttttctg ttcggcgcta tgtgggggat cggtaatatc aactacggcc tgaccatgcg
    19921 ttatctcggc atgtcgatgg gaattggcat cgccattggc attacgttga ttgtcggtac
    19981 gctgatgacg ccaattatca acggcaattt cgatgtgttg attaacaccg aaggcggacg
    20041 catgacgttg ctcggcgttc tggtggcgct gattggcgta gggattgtaa ctcgagccgg
    20101 gcagttgaaa gagcgcaaga tgggcattaa agccgaagag ttcaatctga aaaaaggact
    20161 ggtgctggcg gtgatgtgcg gcattttctc tgccgggatg tcctttgcga tgaacgccgc
    20221 aaaaccgatg catgaagccg ctgccgcact aggcgtcgat ccactgtatg tcgctctgcc
    20281 aagctatgtt gtcatcatgg gcggcggcgc gatcatcaac ctcggtttct gttttattcg
    20341 tctggcaaaa gtgaaggatt tatcgctaaa agccgacttc tcgctggcaa aaccgctgat
    20401 cattcacaat gtgttactct cagcactggg cgggttgatg tggtatctgc aattcttttt
    20461 ctatgcctgg ggccacgccc gcattccggc gcagtatgac tacatcagtt ggatgctgca
    20521 tatgagtttc tatgtattgt gcggcggtat cgtcgggctg gtgctgaaag agtggaacaa
    20581 tgcaggacgc cgtccggtaa cggtgttgag cctcggttgt gtggtgatta ttgttgccgc
    20641 caacatcgtt ggcatgggca tggcgaatta atctttctgc gaattgagat gacgccactg
    20701 gctgggcgtc atcccggttt cccgggtaaa caccaccgaa aaatagttac tatcttcaaa
    20761 gccacattcg gtcgaaatat cactgattaa caggcggcta tgctggagaa gatattgcgc
    20821 atgacacact ctgacctgcc gcagatattg attgatggtc attccagtct gctggcgaaa
    20881 ttgctgacgc aaaacgcgct cactgcacga tgcctcatca caaaatttat ccagcgcaaa
    20941 gggacttttc aggctagccg ccaaccgggt aatcagctta tccagcaacg tttcgctgga
    21001 tgttggcggc aacgaatcac tggtgtaacg atggcgattc agcaacatca ccaactgccc
    21061 gaacagcaac tctgccattt cgttagcaaa cggcacatgc tgactacttt catgctcaag
    21121 ctgaccgata acctgccgcg cctgtgccat ccccatgcta cctaagcgcc agtgtggttg
    21181 ccctgcgctg gcgctaaatc ccggaatcgc cccctgccag tcaagattca gcttcagccg
    21241 ctccgggcaa taaataatat tctgcaaaac cagatcgtta acggaagcgt aggagtgttt
    21301 atcgtcagca tgaatgtaaa agagatcgcc acgggtaatg cgataagggc gatcgttgag
    21361 tacatgcaga ccattaccgc gccagacaat caccagctca caaaaatcat gtgtatgttc
    21421 agcaaagaca tcttgcggat aacggtcagc cacagcgact gcctgctggt cgctggcaaa
    21481 aaaatcatct ttgagaagtt ttaactgatg cgccaccgtg gctacctcgg ccagagaacg
    21541 aaattgatta ttcgcaatat ggcgtacaaa tacgttgaga agattcgcgt tattgcagaa
    21601 agccatcccg tccctgacga atatcacgcg gtgaccagtt aaactctcgg cgaaaaagcg
    21661 tcgaaaagtg gttactgtcg ctgaatccgc aatgataggc gatgtcagta acgctggcct
    21721 cgctgtgacg tagcagatgt cgggctttca tcagtcgcag gcgattcagg tatcgctgag
    21781 gcgtcagtcc cgtttgctgc ttaagctgcc gatgtagcgt acgcagtgaa agagaaaatt
    21841 gatccgccac ggcatcccaa ttcacctcat cggcaaaatg gtcctccagc caggccagaa
    21901 gcaagttgag acgtgatgcg ctgttttcca ggttctcctg caaactgctt ttacgcagca
    21961 agagcagtaa ttgcataaac aagatctcgc gactggcggt cgagggtaaa tcattttccc
    22021 cttcctgctg ttccatctgt gcaaccagct gtcgcacctg ctgcaatacg ctgtggttaa
    22081 cgcgccagtg agacggatac tgcccatcca gctcttgtgg cagcaactga ttcagtccgg
    22141 cgagaaactg aaatcgatcc ggcgagcgat acagcacatt agtcagacac agattatcgg
    22201 tatgttcata cagatgccga tcatggtcgc gcacaaaaca gaccgtgcca ccggtgatgg
    22261 tatagggctg cccattaaac acatgaatac ccgtgccatg ttcgacaatc acaatttcat
    22321 gaaaatcatg atgatgttca ggaaaatccg cctgcgggag ccggggttct atcgccacgg
    22381 acgcgttacc agacggaaaa aaatccacac tatgtaatac ggtcatactg gcctcctgat
    22441 gtcgtcaaca cggcgaaata gtaatcacga ggtcaggttc ttaccttaaa ttttcgacgg
    22501 aaaaccacgt aaaaaacgtc gatttttcaa gatacagcgt gaattttcag gaaatgcggt
    22561 gagcatcaca tcaccacaat tcagcaaatt gtgaacatca tcacgttcat ctttccctgg
    22621 ttgccaatgg cccattttcc tgtcagtaac gagaaggtcg cgaattcagg cgctttttag
    22681 actggtcgta atgaaattca gcaggatcac attatgacct ttcgcaattg tgtcgccgtc
    22741 gatctcggcg catccagtgg gcgcgtgatg ctggcgcgtt acgagcgtga atgccgcagc
    22801 ctgacgctgc gcgaaatcca tcgttttaac aatgggctgc atagtcagaa cggctatgtc
    22861 acctgggatg tggatagcct tgaaagtgcc attcgccttg gattaaacaa ggtgtgcgag
    22921 gaagggattc gtatcgatag cattgggatt gatacctggg gcgtggactt tgtgctgctc
    22981 gaccaacagg gtcagcgtgt gggcctgccc gttgcttatc gcgatagccg caccaatggc
    23041 ctaatggcgc aggcacaaca acaactcggc aaacgcgata tttatcaacg tagcggcatc
    23101 cagtttctgc ccttcaatac gctttatcag ttgcgtgcgc tgacggagca acaacctgaa
    23161 cttattccac acattgctca cgctctgctg atgccggatt acttcagtta tcgcctgacc
    23221 ggcaagatga actgggaata taccaacgcc acgaccacgc aactggtcaa tatcaatagc
    23281 gacgactggg acgagtcgct actggcgtgg agcggggcca acaaagcctg gtttggtcgc
    23341 ccgacgcatc cgggtaatgt cataggtcac tggatttgcc cgcagggtaa tgagattcca
    23401 gtggtcgccg ttgccagcca tgataccgcc agcgcggtta tcgcctcgcc gttaaacggt
    23461 tcacgtgctg cttatctctc ttctggcacc tggtcattga tgggcttcga aagccagacg
    23521 ccatttacca atgacacggc actggcagcc aacatcacca atgaaggcgg ggcggaaggt
    23581 cgctatcggg tgctgaaaaa tattatgggc ttatggctgc ttcagcgagt gcttcaggag
    23641 cggcaaatca acgatcttcc ggcgcttatc tccgcgacac aggcacttcc ggcttgccgc
    23701 ttcattatca atcccaatga cgatcgcttt attaatcctg agacgatgtg cagcgaaatt
    23761 caggctgcgt gtcgggaaac ggcgcaaccg atcccggaaa gtgatgctga actggcgcgc
    23821 tgcattttcg acagtctggc gctgctgtat gccgatgtgt tgcatgagct ggcgcagctg
    23881 cgcggtgaag atttctcgca actgcatatt gtcggcggag gctgccagaa cacgctgctc
    23941 aaccagctat gcgccgatgc ctgcggtatt cgggtgatcg ccgggcctgt tgaagcctcg
    24001 acgctcggca atatcggcat ccagttaatg acgctggatg aactcaacaa tgtggatgat
    24061 ttccgtcagg tcgtcagcac caccgcgaat ctgaccacct ttacccctaa tcctgacagt
    24121 gaaattgccc actatgtggc gcggattcac tctacacgac agacaaagga gctttgcgca
    24181 tgaccactca actggaacag gcctgggagc tggcgaaaca gcgtttcgcg gcagtaggga
    24241 ttgatgtcga ggaggcgctg cgccaacttg atcgtttacc cgtttcaatg cactgctggc
    24301 agggtgatga tgtttccggt tttgaaaacc cggaaggttc gctgaccgga gggattcagg
    24361 ctactggcaa ttatccgggc aaagcgcgta atgccagtga gctacgtgcc gatctggaac
    24421 aggctatgcg gctgattccg gggccaaaac ggcttaattt acatgccatc tatctggaat
    24481 cagacacgcc agtctcgcgc gaccagatca aacctgagca cttcaaaaac tgggttgaat
    24541 gggcgaaagc caatcagctc ggtctggatt ttaacccctc ctgtttctcg catccgctaa
    24601 gcgccgatgg ctttacgctt tcccatgccg acgacagcat tcgccagttc tggattgatc
    24661 actgcaaggc cagccgccgc gtttcggcct attttggtga gcaactcggc acaccgtcgg
    24721 tgatgaacat ctggatcccg gatggcatga aagatatcac cgttgaccgt ctcgctccgc
    24781 gccagcgtct gctggcagct ctggatgagg tgatcagcga gaagctggat ccggcgcacc
    24841 atatcgacgc cgttgagagc aaattgtttg gcattggcgc agagagctac acggttggct
    24901 ccaatgagtt ttacatgggg tatgccacca gccgccagac tgcgctgtgc ctggacgccg
    24961 ggcatttcca cccgactgaa gtgatttccg acaagatttc cgccgccatg ctgtatgtgc
    25021 cgcagttgct gctgcacgtc agccgtccgg ttcgctggga cagcgatcac gtagtgctgc
    25081 tggatgatga aacccaggcg attgccagtg agattgttcg tcacgatctg tttgaccggg
    25141 tgcatatcgg cctcgacttc tttgatgcct ctatcaaccg tattgctgcg tggatcattg
    25201 gtacacgcaa tatgaaaaaa gccctgctgc gtgcgttgct ggaacctacc gctgagctgc
    25261 gcaagctgga agcggcgggc gattacactg cgcgtctggc actgctggaa gagcagaaat
    25321 cgttgccgtg gcaggcggtc tgggaaatgt attgccaacg tcacgatacg ccagcaggta
    25381 gcgaatggct ggagagcgtg cgggcatatg agaaagaaac tttgagtcgc cgcgggtaaa
    25441 cactgccgga tgcggcacaa gtgccttatc cggcctacag gccggcaata gttgtaggcc
    25501 tgataagacg cgacagcgtc gcatcaggca ttgattgccg gatgcggcac gagcgcctta
    25561 tccggcctac gggtcgtgca tctgacaaca ccgaatttac aggaacacag aacatgcaaa
    25621 acattaccca gtcctggttt gtccagggaa tgatcaaagc caccaccgat gcctggctga
    25681 aaggctggga cgagcgcaac ggcggcaacc tgacgctgcg cctggatgac gccgatatcg
    25741 cgctttatca cgacaacttc cacccacaac cgcgctatat tccgctcagc caacccatgc
    25801 ctttactggc aaatacgccg tttattgtca ccggctccgg caaattcttc cgtaacgtcc
    25861 agctcgatcc tgcagctaac ttaggcgtcg taaaagtcga cagcgatggc gcgggctacc
    25921 atattctctg ggggttaacc aacgaagccg ttccaacttc tgaacttccg gctcactttc
    25981 tctcccactg cgagcgcatt aaagccacca acggtaaaga tcgggtgatc atgcactgcc
    26041 acgccaccaa cctgatcgcc ctcacctatg tacttgaaaa cgacaccgcg gtcttcactc
    26101 gccaactgtg ggaaggcagc accgagtgtc tggtggtatt cccggatggc gttggcattc
    26161 tgccgtggat ggtacccggc acggacgaaa tcggccaggc aactgcccaa gagatgcaaa
    26221 aacattcgct ggtgttgtgg cccttccacg gtgtgttcgg ttccggaccg acgctggatg
    26281 aaaccttcgg tttaatcgac accgcagaaa aatcagcgga agtgttagtg aaggtgtatt
    26341 cgatgggcgg catgaaacag actatcagcc gtgaagagtt gatagcgctc ggccagcgtt
    26401 tcggcgttac accactcgcc agtgcgctgg cgctgtaagg agcaaacatg atccgcaaag
    26461 cctttgtcat gcaggtaaac cccgacgccc acgaagagta tcagcgtcgg cataatccca
    26521 tctggccgga actggaagca gtgttgaaat ctcacggtgc gcataactac gccatttacc
    26581 tcgacaaagc gcgtaatctg ctgtttgcca cggtagagat tgaatctgaa gaacggtgga
    26641 atgcggtagc cagcactgaa atctgccaac gttggtggaa atatatgacc gatgttatgc
    26701 ccgctaaccc ggataacagc ccggtgagta gcgagctgca agaagtgttt tacctgcctt
    26761 aatgagttgc ggtgccggaa aatatccagc accgcgactt catcagaaga agtatttaaa
    26821 gcgaacgcgt gcgccgtaac ggtcatcagt atcatcaccg tgttttttat cgccgtcgct
    26881 atccagaatg gagtaatacg tcccgaggta aatattaaag ttctccatat ccatcacgtt
    26941 agcgaactga taagacgcat gaatggtgtg aatggtgtac gtcccttcat catcaatcca
    27001 gcagtcgtta tcacaaacta caccgctgaa ttcatcaatc ttattgtgcg cgtagatata
    27061 accgagctcg aaacgtttcc acagcgcgtt aatcccggca gtgaaatctt tctcattatt
    27121 agcatctaaa taggcggtat ttagattaac cacaatgcca ttgtccggat ccgttttcag
    27181 gccgttccag gtcatgctca taccatagcc ggtacgatcg gactgatcga caaaattacc
    27241 cttgctatcg gtataaccat aagcattatt taccacgttc gcttccatcg ctgcggaaac
    27301 ggtgaattct tccgtcggcg accaggcaat tactgggcgc agataagcaa catttttctg
    27361 ctgctccata tcgcgtccat gataattacc gtcattatat aaagatgttc cgtcttcaag
    27421 taacgtgttt aattcaaaat accagttatc gagttgtttg ctgacgagga aattaccgcc
    27481 agcgttagag cgcccgcggc cctctttcat catatagata taaccgctgc catcgtcata
    27541 aagatcgttc gcagtattac cggaatgttc aacaaaggta tcctgattca atgggaacat
    27601 atcgtaggct tcaaaacggc ctactttgac cttccagtcg ttttctttgc caaagaagaa
    27661 taccgcatca tcgatattta ctgaaccgtg catatcaccc agcggttgtg cggagaaccc
    27721 ggcgaaataa ccgttgtcca tctttcgcat accgtcaaaa cctaacagaa tacgaccatt
    27781 gagattccac tgttcattag tgggatcgct attcacatca gcgttggtca ttgccagcag
    27841 gccatgatta ctttccgcat ccatattaaa ttctacgtcg ccgtagattt ttaaatcgcc
    27901 ataacctgaa agcaaaagat taggtgaggt ggcatttttc tttgcagatt ccgccgttgc
    27961 ctgtccattt accggcgtat tgccctgagc ggccttaatt tcgcggatct cagcggcttg
    28021 ttgtgtctgc agctgtttaa cctggctctc tgcttttgcc gcccgcattt ctgccgcttc
    28081 caggcgttct tccagcaatg ccatgcgttg ttccagcatt aaatgctgtg tttttgccat
    28141 accggtacta catgccaata ataaaccgat ggctaaagat aatgtcgatt ttttcattat
    28201 ttacgttccc tgaaataaag gagaccctac gctttccaaa aggaata
//
LOCUS       DBFIFI010000044        27854 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.044, whole genome shotgun sequence.
ACCESSION   DBFIFI010000044 DBFIFI010000000
VERSION     DBFIFI010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27854
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.044"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            123..785
                     /gene="acrS"
                     /locus_tag="N7B39_001804"
     CDS             123..785
                     /gene="acrS"
                     /locus_tag="N7B39_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HGU2421799.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            complement(788..967)
                     /locus_tag="N7B39_001805"
     CDS             complement(788..967)
                     /locus_tag="N7B39_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HGU2421800.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(1051..1935)
                     /gene="yhdJ"
                     /locus_tag="N7B39_001806"
     CDS             complement(1051..1935)
                     /gene="yhdJ"
                     /locus_tag="N7B39_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HGU2421801.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLLEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLITK"
     gene            complement(2020..2316)
                     /gene="fis"
                     /locus_tag="N7B39_001807"
     CDS             complement(2020..2316)
                     /gene="fis"
                     /locus_tag="N7B39_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HGU2421802.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(2342..3307)
                     /gene="dusB"
                     /locus_tag="N7B39_001808"
     CDS             complement(2342..3307)
                     /gene="dusB"
                     /locus_tag="N7B39_001808"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HGU2421803.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(3636..4517)
                     /gene="prmA"
                     /locus_tag="N7B39_001809"
     CDS             complement(3636..4517)
                     /gene="prmA"
                     /locus_tag="N7B39_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HGU2421804.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(4529..5980)
                     /gene="panF"
                     /locus_tag="N7B39_001810"
     CDS             complement(4529..5980)
                     /gene="panF"
                     /locus_tag="N7B39_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HGU2421805.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5970..6212)
                     /locus_tag="N7B39_001811"
     CDS             complement(5970..6212)
                     /locus_tag="N7B39_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HGU2421806.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(6321..7670)
                     /gene="accC"
                     /locus_tag="N7B39_001812"
     CDS             complement(6321..7670)
                     /gene="accC"
                     /locus_tag="N7B39_001812"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HGU2421807.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNSIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(7681..8151)
                     /gene="accB"
                     /locus_tag="N7B39_001813"
     CDS             complement(7681..8151)
                     /gene="accB"
                     /locus_tag="N7B39_001813"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HGU2421808.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(9129..10103)
                     /locus_tag="N7B39_001814"
     CDS             complement(9129..10103)
                     /locus_tag="N7B39_001814"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HGU2421809.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            10255..12195
                     /gene="csrD"
                     /locus_tag="N7B39_001815"
     CDS             10255..12195
                     /gene="csrD"
                     /locus_tag="N7B39_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HGU2421810.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            12500..13543
                     /gene="mreB"
                     /locus_tag="N7B39_001816"
     CDS             12500..13543
                     /gene="mreB"
                     /locus_tag="N7B39_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HGU2421811.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            13609..14712
                     /gene="mreC"
                     /locus_tag="N7B39_001817"
     CDS             13609..14712
                     /gene="mreC"
                     /locus_tag="N7B39_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HGU2421812.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSLQRATPPQSGAQPPARAPGGQ"
     gene            14712..15200
                     /gene="mreD"
                     /locus_tag="N7B39_001818"
     CDS             14712..15200
                     /gene="mreD"
                     /locus_tag="N7B39_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HGU2421813.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            15209..15802
                     /gene="yhdE"
                     /locus_tag="N7B39_001819"
     CDS             15209..15802
                     /gene="yhdE"
                     /locus_tag="N7B39_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HGU2421814.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKARAGVVQTAKDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSRHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYQAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15792..17261
                     /gene="rng"
                     /locus_tag="N7B39_001820"
     CDS             15792..17261
                     /gene="rng"
                     /locus_tag="N7B39_001820"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HGU2421815.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            17329..21129
                     /gene="yhdP"
                     /locus_tag="N7B39_001821"
     CDS             17329..21129
                     /gene="yhdP"
                     /locus_tag="N7B39_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HGU2421816.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKET
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLGIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPAAVNEALSGSVAWDGKVGIVLPYHAGATYNVELNGDLKNV
                     SSHLPSPLAKPAGEPLPVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTQVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWSDAQIRCAECWFWGQKFGRIDSDITISGNTLTLTNGLI
                     DTGFSRLTADGEWINNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            21285..22730
                     /gene="tldD"
                     /locus_tag="N7B39_001822"
     CDS             21285..22730
                     /gene="tldD"
                     /locus_tag="N7B39_001822"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HGU2421817.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFVILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIARD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLIEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22864..23793)
                     /gene="aaeR"
                     /locus_tag="N7B39_001823"
     CDS             complement(22864..23793)
                     /gene="aaeR"
                     /locus_tag="N7B39_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HGU2421818.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23976..24179
                     /gene="aaeX"
                     /locus_tag="N7B39_001824"
     CDS             23976..24179
                     /gene="aaeX"
                     /locus_tag="N7B39_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HGU2421819.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVCRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            24187..25116
                     /gene="aaeA"
                     /locus_tag="N7B39_001825"
     CDS             24187..25116
                     /gene="aaeA"
                     /locus_tag="N7B39_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HGU2421820.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHNQLVKKGQVLFTIDQPRYQKALEEAQADVAYYQVLAQE
                     KRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAPA
                     DGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGSN
                     KVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWPA
                     GTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            25122..27089
                     /gene="aaeB"
                     /locus_tag="N7B39_001826"
     CDS             25122..27089
                     /gene="aaeB"
                     /locus_tag="N7B39_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HGU2421821.1"
                     /translation="MDIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LCIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            27181..27453
                     /locus_tag="N7B39_001827"
     CDS             27181..27453
                     /locus_tag="N7B39_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HGU2421822.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(27509..27772)
                     /gene="yhcN"
                     /locus_tag="N7B39_001828"
     CDS             complement(27509..27772)
                     /gene="yhcN"
                     /locus_tag="N7B39_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HGU2421823.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         6685 a         6962 c         7416 g         6791 t
ORIGIN      
        1 ttatttaacg atttatagca actcaatgtt agcctctcgt ataaatacac attaggtgat
       61 agattaacct tcgctatttt ctcactctgt gtcgaagata tttatttcct gaataattaa
      121 tcatggcaaa aagaaccaaa gccgaagctc tgaagacccg gcaagaactg attgaaactg
      181 ccatcgccca gtttgcgcaa catggcgtaa gcaagacgac gctcaacgac attgccgatg
      241 ccgctaacgt tacgcgtggc gctatctact ggcacttcga gaacaagacc caactgttta
      301 atgagatgtg gttgcaacag ccttcattgc gggagttaat ccaggaacac ttgacggctg
      361 gattagagca tgacccgttt caacaattgc gtgaaaaatt gattgtcggc ttgcaatata
      421 ttgccaaaat tccccgccag caggcgttgc tgaaaatctt atatcacaaa tgtgaattta
      481 atgatgagat gctggccgag ggagtgatac gcgaaaagat gggctttaat ccgcagactc
      541 tccgcgaagt attgcaggcg tgtcagcaac aaggttgtgt agcaaataac ctcgatttag
      601 atgttgtgat gattattatt gatggtgcct tcagcggaat tgttcaaaac tggttaatga
      661 atatggcggg ttatgatctt tataaacaag cccccgctct ggtcgataac gtattaagaa
      721 tgttcatgcc agatgaaaac ataacgaaat taattcatca aacgaatgaa ttaagtgtca
      781 tgtaaattca cattctgtcc tgaaaattca gttcgtcaca gttaaccagt tttagtggat
      841 taactgagtt catattgcgg catttgtctg tttctgttgc cagcagttca atccactgca
      901 tcagtaaagt atcaaacaga aaaacgaaga cagcgaaaac gacgagccac caatacttgc
      961 gaatcattac ctgagcctgt gcatgtttaa ccaagtctgg caggaaatat acacgaaatg
     1021 aataatctgc aaaccaggct tacacatcct ttactttgta atgagatcgg ggtcaacttc
     1081 gcttaaccgg cttcgttttg acaggttgcc ggtctttctt tttttcactt tcgccagttc
     1141 ttccgcagag taatgcgacg cgacatccag ccgtcgaagc cccattttga tgtactcgct
     1201 gttgatctca ataccaatga attttcgtcc gctggcgatg gctacggcac cggtagtaaa
     1261 gctaccagca aacgggtcga gaacgatatc gcctgggttg gaagaggcga gaataatgcg
     1321 tttcagtaag gcttccggtt tttgcgtcgg gtggttttca tattcatcca tcaaataacg
     1381 cacgcgcgga aaatcccaga cgttacccgg tactttttga tgattgtaag gctgtggagg
     1441 atttttgcga taatcgatca acgcgcgctg cgatccggtt ttggcttcta ccagaatagc
     1501 atcaccgttg aatgtgtagt tctttgcgtc tttcaccatc atcaggatgg gttcgtacat
     1561 ggagccgtag tgttttttcg cctgcactcc agaactgtca tatgaccaga cgatgcgact
     1621 tttgatggta aaaagcttgc ggcactggag atcgataaag ggcatgtttt ccgtactgtt
     1681 catgatgtac atgctgccct gctttttcag aacgcggtgg cactctgcaa tcacttctaa
     1741 cagccagtcg ataaacagat cttctttcca ggcttcgatc agaccatcaa aatttttacc
     1801 gatgttatac ggtgggtcgg caaagatcag atcgacgctt tcagtaggta gctttttaag
     1861 ttcggcaagg gcatcaccgt gaataatggt cttagcttca ttaccaaacc gggtcggttc
     1921 acatcctgtt ctcatggtca ctccctttgt gacacctata aaaaaggcgc ttccccatgc
     1981 cgagtagcgc cttttaatca agcaattagc taacctgaat tagttcatgc cgtatttttt
     2041 caatttttta cgcagcgtac cacggttgat gcccatcatc agcgcagcac gggtctggtt
     2101 accacgggtg tattgcatca ccatgtccaa caggggctgt tctacttcag ccagtaccag
     2161 ctcatagagg tcattcacat cctgaccatt cagttgagca aaatagttct tcagtgcctg
     2221 tttaaccgag tcacgcaggg gtttttgggt tacctgatcc tgagagttaa cggtagaaac
     2281 ggtcagtacg tcagaattta cgcgttgttc gaacatagtt ctgtcagctc tttatttctg
     2341 tttacgcaaa attttcgaag tatgcctcca acgcctccag ctgttcgctg gcatcctcaa
     2401 tggcgttgaa tgtgcgccga aactggtcat ttggagcgtg ttcctggaga taccaggaaa
     2461 cgtgtttacg tgcaattcgg tacccttttg ccggaccata aaagtcatgc agttcccgaa
     2521 cgtgcgcgca aagcaagcgc ttaacctctg ccaaaggcag cgggggcagc aactccccag
     2581 tgtccagata atgctggatt tcccgaaaga tccagggtct tccctgagct gcgcggccta
     2641 tcatcagggc atccgcccct gtatagtcga gcacagctct ggctttaagc gggtcagtaa
     2701 tgtcgccatt cgcgataacc ggaatggaaa ctttctgctt aactgcccga atactgtcgt
     2761 actcagcttc tccattgaac aaacaggcgc gtgtacggcc atgaatggtc agagcctgaa
     2821 tgccacagtc ttcagccagt tgggcaatct cttcgcagtt acggtgttcc ggtgcccagc
     2881 cggtgcgaat cttcagggta acaggaacgt ccactgcatt gacgacctcg gtaaggatcg
     2941 atttaacgac atccgggtac tgcaagaggg ctgaacctgc gagcttgcga ttcacttttt
     3001 tagccgggca acccatattg atatcaataa tctgggcacc gctttccacg ttaatacgtg
     3061 ctgcatctgc catttctttc ggatcgctac cagcaatttg cacggtgcga ataccgggtt
     3121 catcaatgtg caccatccgt aaacgagatt tgtcgctttc ccaaacctgt gggttagaag
     3181 acatcatctc ggatactgtc aatccggctc ccatctcgta gcacaacgtc cgaaaaggtc
     3241 tgtctgtaat gccagccatg ggcgctgcga tcaggcgatt tctgagctga tattgtccga
     3301 tgcgcatgag ttaagaaatg accatactgt gactgcaagg cggcgtatat tacgcatttt
     3361 ttgcacgaga tgaaaggcca aactttgacc aatcctctga gatggatcaa agaattgcat
     3421 ttaaaatgag cgtggtgcga taattactca taaaaatcat catattagaa aatagtgact
     3481 aaaatttaca ctcaaagaaa tttgagtaag ttctcaattt ttctttatga atgaaaattt
     3541 tggcacgcaa atttgcgtaa ataatcggca aatttacgtg ccttttgtga gcttgctcgc
     3601 acttcgcccc gcgtcaccct atggcgatgc gaaggttaat tcttacgacc ggtaatacgg
     3661 caccactctt ctttttccac gaccgggtcc agagcgaagc tgtcggcata ggcttcgcaa
     3721 acgctctccg cctggcttgc cagaatacca gaaaggccca gtaagccgcc tgaaactggc
     3781 aggacgctaa ttaacggtgc cagttcacgt aatgggcctg caaggatgtt agcgaccacc
     3841 acgtcggctt tcatttcttc tggctgatct ttcggtaagt agagttccag acggtcagaa
     3901 acgccattac gttcggcgtt atcgcggctg gcctgaatcg cctgcggatc gatatcaata
     3961 ccaatggctt ttgctgcacc cagtttcagc gccgcgatcg ccagaatgcc ggaaccacag
     4021 ccaaagtcga tgactgtttt accggttaaa tcgaggctgt cgagccattg caggcacaga
     4081 gaggtggttg gatgggtacc cgtaccaaac gccagccctg gatctaacat cacgttgacg
     4141 gcgttttcgt ccggcacatc acgccagcta gggcagatcc acagtcgttc gccaaagcgc
     4201 atcgggtgga aattatccat ccattcgcgc tcccagtctt tatcttctag ttgttcgatt
     4261 ttatgcgcga agcctgcgcc gagcagcgga tggttttcca gaatcgccac cacgtcgttc
     4321 atatcggttt cagcgtcgaa cagaccaatc acatcggtgt cgccccacag gcgcgtttcg
     4381 cccggcagcg gctcaaatac tggcgtatcg tgggtatcct gaaaagtgat agaaacggca
     4441 cccgcttcca tcagcgcatc gctaagatct tccgcgttcg cgccggtggt gttcagtttc
     4501 agttggatcc aaggcatggc aaaactcttt atttatcagt agtcaaaacg gtagcttgcg
     4561 ggacggatgt accgaaacgg tttccgacca ggaaagccag caaacttagt agtaacgagg
     4621 gcacgatagg gtggaagccc aggtactgaa tattcagcgt cgcgagtacg gcatacagca
     4681 cgccgccaac gatcatcgca cttagcgcgc ctttggcgtt ggcgcgttcc cagtaaagac
     4741 ccagcaccag cggccacagg aaaacggctt ccagcccacc gaaggccagc aaattcagcc
     4801 agatgatcat ttctggcggc ttccaggcgg caagcagcag caacgcgccg agaactaacg
     4861 taattaccgc cgacatccgc ttcagacgcg tctcgttttg catttgatcc ggacggatat
     4921 tcagatagag atctttaatg atcgtagcgg aactttgtag caattgggcg ttaattgtcg
     4981 acatgatcgc agccataggt gcagccagaa agatcccggc agcaaacggt ggcagcactt
     5041 ttaccattaa cgtcgggatc accagatccg gtacggtgag atcggggatc actgcacgac
     5101 ctaacgctcc ggctaggtgc ataccgaaca tcaggattgc gactacaatc gtaccgatga
     5161 tgatcccccg gtgtactgct ttgctgtctt tataagagat acagcgcacc gcagtatgcg
     5221 gcaggcctat aacgccaaag cacaccagta cccagaacga cgtcataaag gcaggcgaca
     5281 gaatatcgtc agcgccttgt ggcgtaacca gttgcggatc gatggtttgc aaggtctgta
     5341 ctgcgttgct taagccgcca gcggcatgta ccacgccaat aagcagcaca acggtgccaa
     5401 tcagcatcac aagcccttgc atggtgtcgt tcagcacgct ggcgcgaaag ccaccaaagg
     5461 cggtatataa cgcaatgctg ataccaaaaa tcagcagccc ggtttcataa ggaatacccg
     5521 ccgcggtttc cagcaggcgc gcaccgccga taaactgcac ggtcatcgca ccaacgaacg
     5581 caaccagcaa actcaaactc gccagccaca ccagaagacg actctggtag cgggcaaaca
     5641 gcatatcgtt cagcgtcact gcattgtagc ggcgcgcaag aatcgcaaac ttcttgccga
     5701 gaataccgag tgaaagccag actgccggaa gctgaatcat cgccagcaat acccagccaa
     5761 ggccgtattt gtaagcagct ccaggcccgc cgataaacga actggcactg atataagtcg
     5821 cggtgagcgt catcgccagc acaataccgc ccatagagcg gctgccgagg aaatactcat
     5881 taaggaaggt gccggtgctc cgtttacgca tcgcataaac cgagataccg aacaccacca
     5941 ccagataggc gaccagcggt agaattactt caagctgcat cgtcatcctc cagcgggata
     6001 tcgcgataga taaatttcac catcgcccag cacagtccaa taaacagcag cggcgtcagg
     6061 atgcaggcca tctcaaacca gcgcggaaag ccggtaaaac cgggggcaac gccaggtaag
     6121 taagcggcta ctaaccaaac tgccagatac aaaagggtca gccccagcgc ccagcgcgcc
     6181 tctttatggg cctgaacaaa acgagtgtcc attttttgtc cctgatgggt gaagaaagcg
     6241 aggattgtac cttatgggtg ttgtcgatcc ccagtaataa aaaaggccgg aaaatccggc
     6301 cttttgacgc ttttgcagtc ttatttttcc tgaagaccga gttttttctc cagatagtgg
     6361 atgttagtgc caccatgctg gaagttctcg tcattcatga tgcggatctg cagatcaacg
     6421 ttggttttga taccgtcgat gatcagctcc tgcagcgcat tcttcatgcg ggcaatcgcc
     6481 acgtcacggt tttcaccgta gcaaatcagc ttaccgatca ttgagtcata gtacggcggt
     6541 acggtgtagc ccgcgtagat atgagactcc caacgtacgc caaaaccgcc aggtgcgtgg
     6601 aaacgggtga ttttgcccgg acttggcagg aaggtgttcg gatcttcggc gttgatacga
     6661 cattccaccg catggccgcg aacgtgaact tcttcttgct tgatcgacag cggttgaccg
     6721 gcagcgatac gcagctgttc tttgatcagg tcaacgccgg tgatcatttc tgtaaccggg
     6781 tgttctacct gaatacgggt gttcatttcg atgaaataga actcgccgtt ttcgaacagg
     6841 aactcgaaag tacctgcacc gcgatagccg atatcaacac acgctttagc gcaacgttcg
     6901 ccgatgtagc gacgcagttc cggggtaatg cccggtgctg gtgcttcttc gaccactttc
     6961 tggtggcggc gctgcatgga gcagtcacgt tccgccagat agatagagtt gccctgaccg
     7021 tcagccagta cctgaatctc gacgtggcga ggattttcca ggtatttctc catgtaaacc
     7081 atatcgttgc tgaaagcagc tttcgcttcc gcacgggtca tggagatgga ttgtgccagt
     7141 tcagcgtcgc cgcgcactac gcgcataccg cgaccaccgc cgccgccgga ggctttgata
     7201 atcaccggat aaccaatgcg tttagcaatg gcacggtttt tatccatatc gtcgcccagc
     7261 gggccgtcag aacccggtac gcaagggacg cccgcttttt tcatcgccgc gattgcggat
     7321 actttgtcgc ccatcaggcg aatggtttct gctttcgggc caatgaagat aaagccggag
     7381 cgttcaacct gctcggcaaa gttggcgttc tcggagagga agccgtaacc cggatggatt
     7441 gctactgcgc cggtgatttc agcggcgctg atgattgccg ggatgttcag ataacttttt
     7501 actgacggag cagggccaat acagaccgtt tcatctgcca gtaatacgtg ttttagatcg
     7561 cgatccgcgc tggagtgcac agcgacagtc ttgatgccca gttctttaca ggcacgaaga
     7621 atacgcaatg caatctcgcc gcggttggca ataacaattt tatccagcat gttcgcctcg
     7681 ttactcgatg acgaccagcg gctcgtcaaa ttctaccggt tgtccacttt cgaccagaat
     7741 tgctttcacg gtaccggatt tgtccgcttc gatctggttc atcattttca tggcttcaac
     7801 gatgcacagg gtatcgccca cgttgacttt ctgacccact tcgatgaacg cttttgcatc
     7861 cgggcttggg gtgcggtaga aagtaccaac catcggggaa cgtacgatgt gaccactgat
     7921 ttccgctgct gctggcgctt ccatggaagg aacggtcgcc ggagcggctg cgttagattg
     7981 agctggctgc tgcatcattg gtgcagcgta agcttgttgc atcacaggga aacttgcggc
     8041 aggagctgca cggctaatgc gtactgactc ttcgccttca gaaatttcca gttcggagat
     8101 gcctgattct tcaaccagct cgatcagttt tttaatctta cgaatatcca tgagtgggtt
     8161 ccgtactctt tgtttagtgt gattgtgaca ggcgtttcac cgccgtctgt aaagcgtatg
     8221 ccccgacagc tgtatgcata gcgataaatt ccagcaggtc gggacgccgg tctattttgc
     8281 cccagagtgt caatgttaga cttgacggac attgtgcagc gatctgctgc tatcctccgg
     8341 caaaaaacaa aatatacctt cgttgcgcca atgtcacctt ttccgcagcg caaaaactgc
     8401 gtcagggagg acgaggtcgc acattataac gatttcgtag caattggcag ctaaatactg
     8461 gtcttatcag ggaagataat caacaactaa catgtaaata accttcaaca ccgtgtaatt
     8521 tgcaacaagc cgcacaattc acgaaattag cgccatcatc gacggaactt cttataacgt
     8581 aaggtgaaaa gctgtaaagc cagccctgcg aagatgaggg gctgcaatga gataatcgtc
     8641 acagaccaca aataatgtag ggatgccagg atcgcgacaa gatagacgac attacgcagc
     8701 ggttgctggg gattacccaa ttttcactgc atcgcctgga tagatgtgaa tgctaaagca
     8761 cgcaaaatta accgattgac aatgctcagc attaaatagg ttcgagtgat taatttgtag
     8821 tcaaacaaaa tcagattatt cacctcatgg taccagaaat aagtaagggg ttaagcgtag
     8881 cgtcgcccag gcaaagcacc ataaacctaa caggcgacgg aggcgtatca ataatagctg
     8941 tttagtggag tatcaatgcg catcaattta tggtcagtcg aaatcatgac tttccagttt
     9001 ggatgatgtt gtacaacttc atgaccaagg tgttcgacga aagctgtccg gctaaccgct
     9061 tttcccgtta accgattgaa taatgatttc aaaatgagtt ccctctctta ttattccttg
     9121 ctaaatggtt agttaacctt caccagcgtg cgaccctgga tctggttatt aatgatggcc
     9181 tcggcaaagt tcggtgcctc tgacagagat atctcttttg ccgcctgggt atagaatgat
     9241 tccggtaaat cggcgaccag tcgctgccag gcttgtgcgc ggcgttctgg tggcgtcatt
     9301 actgaatcca ccccttgcaa acggacatta cgcagaataa atggcatgac cgtggttggc
     9361 agagtaaaac cacccgccag accacaggcc gccacgcagc cgccgtaatt catttgcgcc
     9421 agcacttttg ccagcacttt gtcgccaaca gtgtcaattg ccccagccca gacttgtttt
     9481 tccagaggac gggattcggc aaactcatca cgagggagaa tacggctggc acctaaactt
     9541 ttcagatatt catgggtact ttcgcgaccg gaaacggcaa cgacctgata acccaactta
     9601 tgcagcagcg ccacggcggt actgccgacg ccaccactgg cacccgtcac gacaatctcc
     9661 ccgtcctgcg ggcgaacacc ggcatcttcc agcgccatca cacacagcat ggcggtaaaa
     9721 ccggcagtac cgataatcat tgctttacgc gcgtccagcc cttgcggcat ggcaaccagc
     9781 cagtcacctt tcactcgcgc ctgctccgcc agcccacccc agtggttttc accaacgccc
     9841 cagccagtga gtaacacctc ctgaccggca tgaaaacgcg gatcttcgct ggtgcgtaca
     9901 gttccggcaa aatcgatccc aggaatcatc ggaaaattac ggatgatttt tcccttaccg
     9961 gtaatcgcca gcgcatcttt atagttcagg ctcgaccagt gaacatcgac cgtgacatcg
    10021 ccctccggca ggcgactttc gtccagagtc tgtactgatg cgagagtttt gccgtcctgc
    10081 tgttctaaaa gtaacgcctg cataagtggt cctcatgtgc atgatggatt ggaaaataat
    10141 ttctgaagac tatactcgct aatggaaaag caaagccgat gaagcgcaat aaattgcgag
    10201 ataaatctga tttgctagta tgcccgcttc ctcactatcg gagttaacac aaggatgaga
    10261 ttaacgacga aattttcggc ctttgttacg ctgctcaccg ggttaacaat ttttgtgact
    10321 ttgctgggct gttcgctaag tttctacaac gccattcagt ataagtttag tcatcgcgtt
    10381 caggcggtgg cgacggcgat tgatacccac cttgtgtcga atgacttcag cgcattaagg
    10441 ccacaaatta ccgaattaat gatgtcggca gatatcgttc gtgtagacct gctccatggt
    10501 gataagcagg tttataccct ggccagaaat ggtagttatc gtccggttgg caccagcgat
    10561 ctgtttcgtg aactgagcgt tccgttgata aagcatccgg ggatgtcgtt gcgtctggtt
    10621 tatcaggatc cgatgggcaa ctatttccat tcgttgatga ccaccgcgcc gctcacaggg
    10681 gcgattggct ttatcattct tatgctcttt ctggcggtac gctggttaca acggcaactt
    10741 gccgggcaag aattgctgga aacccgggct actcgtatct taaacggtga gcgtggctct
    10801 aatgtgttgg gaaccatcta tgaatggccg cccagaacca gcagtgcgct ggatacgctg
    10861 cttcgtgaaa ttcagaacgc acgcgaacaa cacagccgtc ttgatacgct gatccgctct
    10921 tatgccgcac aggacgtgaa aaccggcctc aataaccgac tctttttcga taatcagtta
    10981 gcaacgttac tggaagatca ggagaaagta ggtacccacg ggatcgtgat gatgattcgt
    11041 ctgccggatt tcaatatgtt gagcgatacc tgggggcaca gccaggttga agaacagttc
    11101 ttcactctga cgaatctgct gtcgacattt atgatgcgct accctggcgc actgctggcg
    11161 cgttaccacc gcagtgattt tgctgcgctg ttaccgcacc ggacgttaaa agaggcagag
    11221 agcatcgccg gtcagttaat caaagccgtc gataccttgc cgaacaataa aatgctcgat
    11281 cgcgacgata tgatccacat tggtatctgc gcctggcgta gtggtcagga taccgagcag
    11341 gtaatggaac atgcagagtc tgccacgcgt aatgcgggat tgcagggcgg caatagctgg
    11401 gctatttacg atgactcgtt gcctgaaaaa ggacgcggta atgttcgctg gcgtacgctt
    11461 atcgagcaaa tgctcagtcg cggcggcccg cgcctttatc aaaaaccggc ggttactcgc
    11521 gaaggtcagg ttcatcatcg cgaactcatg tgccgcatct ttgatggtaa tgaagaggtt
    11581 agctcggcgg agtatatgcc gatggtcttg cagtttggct tatcggaaga gtatgaccgt
    11641 ctgcaaatca gccgtcttat tccactattg cgttactggc cagaggaaaa tctggcgatt
    11701 caggttaccg ttgagtcgct gattcgcccg cgttttcagc gttggctgcg cgatacgtta
    11761 atgcaatgtg aaaaatcaca acgaaaacgc ataattattg aacttgcaga ggccgatgta
    11821 ggtcaacata tcagtcgttt acaacctgtt attcgtttag tgaatgcttt aggggtacgg
    11881 gtagccgtca accaggctgg tttgacgctg gtaagcacca gttggatcaa agaacttaat
    11941 gttgagttac tcaagctcca tccggggctg gtcagaaaca ttgagaagcg aacggagaac
    12001 cagctgctgg ttcaaagcct ggtggaagcc tgctcaggga ccagcaccca ggtttacgcc
    12061 accggtgtgc gttcgcgaag cgagtggcag accctgattc agcgcggtgt tacaggcggg
    12121 caaggggatt ttttcgcgtc ctcacagcca cttgatacta acgtgaaaaa atattcacaa
    12181 agatactcgg tttaacctgc cgtttaatcc gttttcacgt agaataacgc gcgctgcgtc
    12241 tcatgggagt gtgcttgtct gctcgccaga ttgttgcagc acatatgcag atgaatgacc
    12301 ttacgcggtt gcaaacaggc gaggaatgct gctgatgcat taagcctttc tggactcagg
    12361 cagagatttg taacaaagga aacgaactgc actaattttc accgtagcag atgatttttg
    12421 cgccttgtcg ctgctgcgtg tggttggtaa agtaagcgga ttttcttttc cgccccagct
    12481 ttcaggatta tcccttagta tgttgaaaaa atttcgtggc atgttttcca atgacttgtc
    12541 cattgacctg ggtactgcga ataccctcat ttatgtaaaa ggacaaggca tcgtattgaa
    12601 tgagccttcc gtggtggcca ttcgtcagga tcgtgccggt tcaccgaaaa gcgtagctgc
    12661 agtaggtcat gacgcgaagc agatgctggg ccgtacgccg ggcaatattg ctgccattcg
    12721 cccaatgaaa gacggcgtta tcgccgactt cttcgtgact gaaaaaatgc tccagcactt
    12781 catcaaacaa gtgcacagca acagctttat gcgtccaagc ccgcgcgttc tggtttgtgt
    12841 accggttggc gcgacccagg ttgaacgccg cgcaattcgt gaatccgcgc agggcgctgg
    12901 tgcccgtgaa gtcttcctga ttgaagaacc gatggctgcc gcaattggtg ctggcctgcc
    12961 ggtttctgaa gcgaccggtt ctatggtggt tgatatcggt ggtggtacca ctgaagttgc
    13021 tgttatctcc ttgaacggcg tggtttactc ctcttccgta cgcattggtg gtgaccgttt
    13081 cgacgaagct atcatcaact atgtgcgtcg taattacggt tctctgatcg gtgaagccac
    13141 cgcagaacgt atcaagcacg aaatcggttc ggcttatccg ggcgatgaag tccgtgaaat
    13201 cgaagttcgt ggccgtaacc tggcagaagg tgttccacgc ggttttaccc tgaactccaa
    13261 cgaaatcctc gaagcactgc aggaaccgct gaccggtatt gtgagcgcgg taatggttgc
    13321 actggaacag tgcccgccgg aactggcttc cgacatctcc gagcgcggca tggtactcac
    13381 cggtggtggc gcactgctgc gtaaccttga ccgtttgtta atggaagaaa ccggcattcc
    13441 agtcgttgtt gctgaagacc cgctgacctg tgtggcgcgc ggtggcggca aagcgctgga
    13501 aatgatcgac atgcacggcg gcgacctgtt cagcgaagag taatcggatg caggcagggg
    13561 aagcgtctgt ttaccctgcc tggtctgata cgagaatacg cataacttat gaagccaatt
    13621 tttagccgtg gcccgtcgct acagattcgc cttattctgg cggtgctggt ggcgctcggc
    13681 attattattg ccgacagccg cctggggacg ttcagtcaaa tccgtactta tatggatacc
    13741 gccgtcagtc ctttctactt tgtttccaat gctcctcgtg aattgctgga tggcgtatcg
    13801 cagacgctgg cctcgcgtga ccaactagaa cttgaaaatc gggcgttacg tcaggaactg
    13861 ttgctgaaaa acagtgaact gctgatgctt ggacaataca aacaggagaa cgcgcgtctg
    13921 cgcgagctgc tgggttcccc gctgcgtcag gatgagcaga aaatggtgac tcaggttatc
    13981 tccacggtta acgatcctta tagcgatcaa gttgttatcg ataaaggtag cgttaatggc
    14041 gtttatgaag gccagccggt catcagcgac aaaggtgttg ttggtcaggt ggtggccgtc
    14101 gctaaactga ccagtcgcgt gctgctgatt tgtgatgcga cccacgcgct gccaatccag
    14161 gtgctgcgca acgatatccg cgtaattgca gccggtaacg gttgtacgga tgatttgcag
    14221 cttgagcatc tgccagcgaa tacggatatt cgtgttggtg atgtgctggt gacctccggt
    14281 ctgggcggtc gtttcccgga aggctatccg gtcgcggttg tctcttccgt aaaactcgat
    14341 acccagcgcg cttatactgt gattcaggcg cgtccgactg cagggctgca acgtttgcgt
    14401 tatctgctgc tgctgtgggg ggcagatcgt aacggcgcta acccgatgac gccggaagag
    14461 gtgcatcgtg ttgctaatga acgtctgatg cagatgatgc cgcaggtatt gccttcgcca
    14521 gacgcgatgg ggccaaagtt acctgaaccg gcaacgggga tcgctcagcc gactccgcag
    14581 caacccacga caggaaatgc agctactgcg cctgctgcgc ccacacagcc cgctgctaat
    14641 cgctctctac aaagggctac gccgccgcaa agtggtgctc aaccgcctgc gcgtgcgccg
    14701 ggagggcaat agtggcgagc tatcgtagcc agggacgctg ggtaatctgg ctctctttcc
    14761 tcattgcgct tttgctgcaa atcatgccct ggccggataa cctgattgtt ttccggccaa
    14821 actgggtgtt actcatcttg ttgtattgga tcctggcctt gccccatcgc gtaaatgtgg
    14881 gcacaggttt tgtgatgggt gccatactgg atctgatcag cggctcgacg cttggcgtac
    14941 gcgtattggc gatgagcatc attgcttacc tggtggcgct gaaataccag cttttccgca
    15001 acctcgcatt atggcagcag gcgctggtcg tcatgttgct ttcgctggtg gtggatatta
    15061 ttgttttctg ggcagagttt ttagtgatta acgtctcttt cagaccggaa gtgttctgga
    15121 gtagtgtagt caatggggtg ctctggccgt ggattttctt gctgatgcgt aaagtccgtc
    15181 agcagtttgc agtgcaataa aggtttctat gacttctctg tatttagctt ccggttctcc
    15241 gcgtcgtcag gagttacttg cgcaacttgg cgtgaccttt gaacgtattg ttacgggcat
    15301 tgaggagcag cgtcagccgc aggagagcgc gcagcagtat gttgtgcgtc tggcgcgcga
    15361 gaaagcacgg gcaggtgtcg tgcaaacggc gaaggatctc ccggtgctgg gtgcggatac
    15421 tatcgttatc ctgaacggag aagtgctgga gaaaccgcgc gacgcagagc atgcggcgca
    15481 gatgttgcgc aaattatcgg gtcagaccca tcaggtgatg acggcagtgg cgttggccga
    15541 cagccggcac attctcgatt gcctggtggt caccgatgtg actttcagaa cgttaacaga
    15601 cgaagacatc gcgggctatg tcgccagcgg tgaaccgtta gataaagcag gtgcatacgg
    15661 tattcagggg ctgggtggct gttttgtcag gaagataaat ggcagctatc aagccgtagt
    15721 cggcttaccg ctggttgaaa cgtatgaatt attaagtaat tttaacgcac tgcgtgagaa
    15781 aagggataaa catgacggct gaattgttag taaacgtaac gccttcggaa acgcgagtgg
    15841 cgtatattga tggcggtatt ctgcaggaaa ttcatattga acgtgaggcg cgacgcggaa
    15901 tagtaggcaa tatctacaag ggtcgtgtaa gtcgtgtact tccgggtatg caggcggctt
    15961 ttgtagatat tgggctggat aaagccgcgt ttcttcatgc atctgacatc atgccgcaca
    16021 ccgaatgtgt ggcgggtgaa gaacaaaagc aattcacggt gcgcgacatc tcggaactgg
    16081 ttcgtcaggg gcaagatctg atggtgcagg tggtgaaaga tccgcttggc actaaggggg
    16141 cgcgcctgac caccgatatc acgctgcctt ctcgctatct ggtctttatg ccaggggctt
    16201 ctcacgttgg ggtttcccaa cgaattgaaa gcgaatcaga acgtgaacgc ctgaaaaaag
    16261 tggtcgcaga gtattgcgac gagcagggcg ggtttatcat ccgtaccgca gcggaagggg
    16321 ttggcgaggc tgaactggcc tccgatgccg cttatctgaa acgcgtctgg accaaagtta
    16381 tggagcgtaa aaagcgcccg cagacccgtt atcagctgta cggcgaactg gcgttggcgc
    16441 agcgtgttct gcgtgatttc gccgatgccg aactggaccg cattcgcgtt gactcacgcc
    16501 tgacttacga agcgttactt gagttcacct cggagtacat tcccgagatg accagcaagc
    16561 tggagcatta caccggacgc cagccgattt tcgatctctt tgatgtcgaa aacgaaatcc
    16621 agcgagcgct ggaacgcaaa gttgaactga aatccggtgg ttatctgatt atcgaccaga
    16681 ccgaagcgat gaccaccgtg gacatcaata ccggagcgtt tgtcggtcat cgcaatctgg
    16741 acgacaccat tttcaatacc aatattgaag cgacgcaggc tatcgctcgc cagttacggt
    16801 tgcgtaatct gggcgggatt atcattattg atttcatcga tatgaataat gaagatcacc
    16861 gccgccgagt gctgcactcg ctggagcagg cgttgagcaa agaccgggtg aaaaccagcg
    16921 ttaatggttt ttcggcgctg gggctggtgg agatgacgcg taaacgcacc cgcgaaagca
    16981 ttgagcacgt actgtgtaac gaatgcccaa cctgccacgg tcgcggaacg gtgaaaaccg
    17041 tggaaacggt atgctatgaa atcatgcgcg agattgttcg tgtacaccat gcttacgact
    17101 ccgaccgttt cctggtctat gcttctccgg cagtagccga agccttgaaa ggcgaagagt
    17161 cacactcgct ggcggaagtg gaaattttcg ttggcaaaca ggttaaagta caaattgaac
    17221 cgctctataa ccaggagcag tttgacgtcg taatgatgta aacagatgct gggccgccat
    17281 ccggcaaagg gtttttgagt cacattttta gcagacaagg agtgacgggt gaggcgattg
    17341 ccggggattt tactgcttac tggagccgcg ctcgttgtga tcgctgccct gctggttagc
    17401 ggcctgcgta ttgctttacc gcatcttgac gcctggcgtc cggaaatcct caacaaaata
    17461 gaatccgcga ctggcatgcc ggtagaagcc agtcagctct cagccagctg gcagaatttt
    17521 ggcccgacgc ttgaagcgca cgacatccgt gcagaactaa aggatggcgg cgaattttcg
    17581 gttaaacgcg ttactctggc gctggatgtc tggcagagcc tgttacatat gcgctggcag
    17641 tttcgcgacc tcactttctg gcagctgcgc tttcgcacca acactcctat caccagcggt
    17701 ggtagcgatg acagtctgga agccagtcac atcagcgatc tgtttcttcg tcaatttgac
    17761 catttcgatc tccgcgacag tgaagtcagt ttcctgacgc catccggtca gcgcgccgag
    17821 ctggcgatcc cacaactcac ctggctgaac gatccacgtc gacaccgtgc ggaaggcctg
    17881 gtaagcctct ccagccttac cggacagcac ggcgtgatgc aggtgcgcat ggatttgcgc
    17941 gatgatgagg ggttgttaag caatggtcgc gtctggctcc aggcggatga catcgacctg
    18001 aagccgtggc tcggtaaatg gatgcaggac aatattgcgc tggaaacggc gcagttctcc
    18061 cttgaaggct ggatgacgat cgacaaaggc gatgtaaccg gcggtgacgt ctggctgaaa
    18121 cagggtggtg ccagctggtt gggcgagaag gaaacgcata cgctgtcggt ggataatctg
    18181 accgcgcata ttacgcgtga aaatccggga tggcagttct ctattcccga tacacggatc
    18241 acgatggacg gcaaaccctg gccgagcgga gcattgacgc tggcctggat accggaacag
    18301 gacgttggcg gcaaagacaa taaacgcagt gacgaactcc ggattcgcgc cagtaatctg
    18361 gagctggcag gcctggaggg catacgcccg ctggccgcga aactttcacc tgcactgggt
    18421 gatgtttggc gctctacaca accgagcggc aagattaaca ctctggcgct ggatatcccg
    18481 cttcaggcgg cagacaagac ccgttttcag gcatcgtgga gcgatctggc ctggaagcaa
    18541 tggaaattat taccgggtgc ggaacacttc tccgggacgc tttccggcag cgttgaaaat
    18601 ggtttgctta ccgcgtcgat gaagcaggca aagatgcctt acgaaacggt attccgtgcg
    18661 ccactggaaa tcgccgacgg ccaggcaact ataagctggc tgaacaatga caaaggtttc
    18721 cagctggatg ggcgtaatat tgacgttaaa gccaaagccg tccatgcgcg cggcggtttt
    18781 cgttacctgc aacctgctaa cgatgaaccc tggctgggta ttctggctgg catcagtacc
    18841 gatgatggtt cacaagcctg gcgctatttc ccggaaaact tgatgggtaa agacctggtt
    18901 gattacttaa gtggcgcgat tcagggcggt gaagcggata acgcgacgct ggtttatggt
    18961 ggcaatccgc aactcttccc ctataaacac aacgaaggtc agtttgaagt gctggtgccg
    19021 ctgcgcaacg cgaagtttgc cttccagccg gactggcctg cattaactaa ccttggtatt
    19081 gaactggact ttattaacga cggtttatgg atgaaaaccg atggcgttaa tctgggcggc
    19141 gtgcgcgcga gtaatctcac cgcagtgatc cctgactact caaaagaaaa actgctgatt
    19201 gacgctgaca ttaaaggtcc gggtaaagcc gttggccctt actttgatga gacaccgctg
    19261 aaagattctc tgggtgcgac cctgcaagaa ctccagctcg acggcgatgt gaatgctcgc
    19321 ttacatcttg atatcccgct gaacggcgag ctggtaaccg cgaaaggtga agtgacgctg
    19381 cgtaataaca gtctgtttat caaaccactc gacagcaccc tgaaaaattt gagcggtaaa
    19441 ttcagcttta tcaatggcga tctgcaaagt gaaccactga cagcaagctg gtttaatcag
    19501 ccgttgaacg tggatttttc caccaaagaa ggggcaaaag cctaccaggt cgcggtgaat
    19561 ctcaacggta actggcaacc ggcgaaaacc ggcgttctgc ctgcagcggt gaacgaagca
    19621 ttgagtggca gcgtggcgtg ggatggtaaa gtgggcattg ttctgcctta tcatgctggc
    19681 gcgacgtata acgtagagct aaacggcgat ttgaagaatg tgagcagtca cttaccttca
    19741 ccgttagcca aacctgcggg tgaaccactg ccggtaaacg ttaaggttga tggcaatctc
    19801 aacagctttg aattaaccgg acaggctggt gcggataatc atttcaatag ccgctggttg
    19861 ctcggtcaaa agctgacgct cgaccgtgct atttgggcgg cagacagtaa aacgctcccg
    19921 ccgttgccgg aacaaagtgg tgttgaactc aatatgccgc cgatgaatgg tgccgagtgg
    19981 ctggccctgt ttcagaaagg tgcggcggag agtgtcggtg gtgcagcgag tttcccacaa
    20041 cacataacgt tacgtacgcc tatgttgtcg ctgggaaatc agcaatggaa taacctgagt
    20101 attgtttcgc aaccgacggc aaatggcacc caggttgagg cgcaggggcg tgaaatcaac
    20161 gccacgctgg cgatgcgtaa taacgcgccg tggctggcga atatcaaata tctttattac
    20221 aacccgagcg tggcgaaaac tcgtggtgat tcaacgccgt catcaccttt cccgacaacg
    20281 gaacgcatta acttccgtgg ctggtcggac gcccaaatac gatgcgcaga gtgctggttc
    20341 tgggggcaaa aattcgggcg cattgacagt gatatcacca tttctggcaa tacattaacg
    20401 ctgaccaatg gactgattga tactggtttt tcgcggctca ctgccgatgg tgaatggatt
    20461 aacaatccgg ggaatgaacg tacctcgctg aaaggaaaac tgcgcgggca gaaaattgat
    20521 gccgccgcag aattttttgg tgtcacgacg cccatacggc agtcgtcatt taatgtggat
    20581 tacgatttac actggcgtaa agcaccgtgg cagccagatg aggcgacgtt gaatggcatc
    20641 attcatactc aactgggtaa aggcgaaatt accgaaatca ataccggaca tgccgggcaa
    20701 ttgctgcgct tattgagcgt agatgccctg atgcgtaagc tgcgttttga tttcagagac
    20761 acttttggcg aagggttcta ttttgactcc attcgcagca ccgcgtggat taaagacggc
    20821 gttatgcaca ccgacgacac gctggtggat ggcctggagg cggatatcgc catgaaaggg
    20881 tcggtaaatc tggtacgtcg cgacctgaat atggaagcgg ttgtcgcacc agagatttct
    20941 gcgacagtgg gcgtggctgc ggcttttgcg gttaacccca ttgttggcgc ggcagtgttt
    21001 gccgccagta aagtgctggg gccgctgtgg agcaaagtct ccattttgcg ctatcacatt
    21061 tcgggtccgc tggacgatcc gcaaatcaac gaagtgttgc gccaaccgcg taaagaaaaa
    21121 gcgcaatgat ttgacgaggg cgcgtaattg ccccaatctc ataggataat cgttgccaaa
    21181 ggccaacgag ccagaacata accgtaggct ggatagggcg ttcacgccgc atccggcagc
    21241 cgtaaaaaat cctctactgc agtaactaac gagtagcaaa aacgatgagt cttaacctgg
    21301 taagtgaaca attgctagcg gcgaacggcc tgaaacatca ggacttgttc gtgatcctcg
    21361 gtcaactggc cgaacgtcgc cttgattatg gcgatctcta ttttcagtcg agctatcacg
    21421 aatcctgggt tttagaagac cgcattatta aagatggttc ttacaacatc gatcagggcg
    21481 ttggtgtgcg tgcaatcagc ggtgaaaaaa ccggatttgc ttacgctgac caaatcagcc
    21541 tgctggcgct ggaacagagt gcgcaagcgg cgcgcaccat cgcccgtgat agcggtgatg
    21601 gcaaagtaca gacgctgggc gcggtagagc atagcccgtt gtatacctcg gtagatccgc
    21661 tgcaaagcat gagccgtgaa gagaagctgg atatcctgcg tcgcgtcgat aaggttgccc
    21721 gcgaagcgga caagcgcgta caggaagtga ctgccagcct cagtggtgtc tatgaattaa
    21781 ttctggttgc ggccaccgac ggcacgctag cggcggatgt ccgtccgctg gtgcgtcttt
    21841 ccgtgagcgt tctgatcgaa gaagatggca aacgcgaacg cggtgccagt ggcggcggcg
    21901 gtcgttttgg ttatgagttc ttccttgccg atctcgacgg cgaagtccgt gcggatgcat
    21961 gggcaaaaga agcagtacgt atggcgctgg tcaatctttc tgccgttgct gcaccagcgg
    22021 gcaccatgcc ggtagtactt ggcgcaggtt ggccgggcgt gctgttgcat gaagcggtag
    22081 gtcacggtct ggaaggcgac ttcaaccgcc gtggcacttc agtatttagt ggacaggtcg
    22141 gggagctggt ggcttcagaa ctgtgtaccg tggttgatga cggtacgatg gtcgatcgcc
    22201 gtggttcggt ggcgattgat gacgaaggta cgccaggcca gtacaacgtg ctgattgaga
    22261 acggcattct gaaaggctac atgcaggata aactcaacgc gcgtttgatg gggatgacgc
    22321 cgactggcaa cggtcgccgt gaatcctacg cccatctgcc catgccgcgt atgaccaaca
    22381 cctatatgct gccgggtaaa tcgaccccgc aggaaattat tgaatccgtt gagtacggta
    22441 tctatgcacc gaactttggt ggcggtcagg tggatatcac ctccggcaaa ttcgttttct
    22501 ccacttcaga agcatatctg attgaaaacg gtaaagtaac gaagccggtg aaaggcgcaa
    22561 cgttgattgg ttccggtatc gaaaccatgc agcagatttc gatggttggc aacgacctga
    22621 aactggataa cggcgtgggt gtctgcggta aagaagggca aagtttgccg gttggcgtgg
    22681 gccagccaac gctgaaagtt gataacctga ctgttggcgg tactgcgtaa taatttcagt
    22741 attttcagaa ggataattaa tccttcctcg tgcacgaacg gtcccctcgc ccctttgggg
    22801 agagggttag ggtgagggga acccgttggc acaggtttgt acaacgtaac agtacaatat
    22861 gaattacttc tctttcccgc gcccgtgcat ctcctgaaac aatttaccga cctcaacaaa
    22921 ataatccgtc agcgagttga tcacgacctg taccttcagc ggcagcttat ctttttcggt
    22981 atataacgca taaaccgggc gtggatctga ctgataacgc ggcagcagga tctccagctc
    23041 cccacgattg atctcgttga tcacccacat cagcggcacg taggcgatcc cggcacccgc
    23101 cgttagccag cgcaccagcg tcatcggatc attagtcaca aatctccctt gcgggatcag
    23161 gcgagtcgag atcccttccg gtgcaatcag ttcaaattca ttgtcgggcc gcacgctgta
    23221 ttcaagccat gaatggctac ttaaatcggc gggtttttcc ggtatgccgt attgtgtgag
    23281 atagcttttc gcggcgcaca ccaccattgg catcgcgcca agacggcggg aaaacaggct
    23341 ggaatcctgc aacgcgccga cgcggatcac cacatccaga ccgtcggcaa tcaggtcggg
    23401 ggctggaatt ccggtaacca gattgacgct caaacctggg tattctttca gcattttggc
    23461 tgtcagcccg gcgagaacat tttgtgccat agttgaagaa cagccaatgc gtagcgtccc
    23521 gatgggggtg ttattgaagg catacagttg ctcatgaaca tcctgcactt catgaagcat
    23581 acgacggcag ccctggtagt aaattctacc ggcttcggtc aggccaatgc tgcgtgtgct
    23641 acggtttaac agctttacct gcaactcatc ttccagtttt gacaccgtct gactgatgga
    23701 cgaaacgctc atctgtagct gtctggcggc ggcggtaaaa gagccaaatt caactacttt
    23761 ggcaaacacc gacatgcgtt ttagtcgttc cattattcac tctgacttaa aagtgattta
    23821 gatcacataa tatagataac agcataacag ttacgctaat atattaaata tcaatctaca
    23881 gcaatgttgc tctcgcccgg ctttccatgc ccttctctgc ggcgacagat gctgaaaata
    23941 ataacgcctg ctctctctat acaaccaagg tcaacatgag tctgtttccc gttatcgtgg
    24001 tgtttgggct gtccttccca ccgatatttt ttgaattgct tttatcactg gcgattttct
    24061 ggctggtgtg ccgggtactt gtgccaacag gtatctacga ctttgtctgg catccggcgt
    24121 tgttcaacac cgcgctctat tgctgcttgt tttatttgat atcgcgactg ttcgtttgag
    24181 gttgaagtga aaacactaat aagaaaattc tcccgtacgg ccatcacggt cgtattagtc
    24241 attctggcct tcatcgcaat ttttaatgcc tgggtctatt acaccgaatc cccctggacg
    24301 cgtgacgcgc gctttagcgc tgacgtcgtt gcgatcgcgc cggacgtttc tggactcatt
    24361 acccaggtga atgttcataa ccagctggtg aaaaaaggac aggttctgtt caccatcgac
    24421 cagccgcgct atcaaaaggc gcttgaggaa gcgcaagccg atgttgctta ttatcaggta
    24481 ctggcacagg agaaacgcca ggaggccgga cgtcgtaacc gtctcggtgt gcaggcgatg
    24541 tctcgcgaag agatcgacca ggccaacaac gtactacaaa cggttctgca tcagttagcg
    24601 aaagcgcagg cgacccgcga tctggcaaaa ctggatcttg aacgcacggt gatccgcgcg
    24661 ccagcagatg gctgggtgac caacctcaac gtctataccg gtgagtttat tactcgtgga
    24721 tcaacggcgg ttgcgctggt gaaacagaac tctttctatg tactggccta tatggaagaa
    24781 actaagctgg aaggggtgcg ccctgggtat cgtgcagaga tcacgccgct tggcagtaac
    24841 aaagtgctga aagggactgt tgatagtgtt gccgctgggg tcaccaacgc cagcagcacg
    24901 cgtgacgaca aagggatggc gaccatcgac tctaaccttg aatgggtgcg tttggcgcaa
    24961 cgtgttccgg ttcgtattcg tctcgacaac cagcaagaga acatctggcc tgcgggcacc
    25021 actgctacag tggtggtcac tggcaaacaa gatcgcgacg aaagccagga ttcgttcttc
    25081 cgtaaaatgg cccatcgcct gcgtgagttt ggttaatcac gatggatatt ttctccattg
    25141 ctaaccaaca tattcgcttt gcggtaaaac tggcgaccgc cattgtactg gcgctgtttg
    25201 ttggctttca cttccagctg gaaacgccac gctgggcggt actgacagcg gcgattgttg
    25261 ccgccggtcc ggcctttgct gcgggaggtg aaccgtattc tggcgctatt cgctatcgtg
    25321 gctttttgcg catcatcggc acatttattg gctgtattgc cggactggtg atcatcattg
    25381 cgatgatccg cgcaccatta ttgatgattc tggtgtgctg tatctgggcc ggtttttgta
    25441 cctggatatc ctcgctggta cgaatagaaa actcgtatgc gtgggggctg gccggttata
    25501 ccgcgctgat cattgtgatc accattcagc cggaaccatt gcttacgccg cagtttgccg
    25561 tcgaacgttg tagcgagatc gttatcggta ttgtgtgtgc gattatggcg gatttgctct
    25621 tttctccgcg atcgatcaaa caagaagtgg atcgagagct ggaaagtttg ctggtcgcgc
    25681 aatatcaatt aatgcaactc tgtatcaagc atggcgatgg tgaagttgtc gataaagcct
    25741 ggggcgacct ggtgcgacgc accacggcgc tacaaggcat gcgcagcaac ctgaatatgg
    25801 aatcttcccg ctgggcgcgg gccaatcgac gtttaaaagc gatcaatacg ctatcgctga
    25861 cgctgattac ccaatcctgc gaaacttatc ttattcagaa tacgcgcccg gaattgatca
    25921 ctgatacttt ccgcgaattt tttgacacgc cggtagaaac cgcgcaggac gtccacaagc
    25981 agctcaaacg cctgcgaaga gttatcgcct ggaccgggga acgggaaacg cctgtcacca
    26041 tttatagctg ggtcgcggcg gcaacgcgtt atcagcttct caagcgcggc gttatcagta
    26101 acacaaaaat caacgccacc gaagaagaga tcctgcaagg cgaaccggaa gtcaaagtag
    26161 agtcagccga acgtcatcat gcgatggtta acttctggcg aaccacactt tcctgcattc
    26221 tgggaacgct tttctggctg tggacgggct ggacttccgg cagtggtgca atggtgatga
    26281 ttgcggtcgt gacgtcactg gcaatgcgtt tgccgaatcc acgcatggtg gcgatcgact
    26341 ttatctacgg gacgctggcc gcgctgccgt tagggctgct ctactttttg gtgattatcc
    26401 ctaataccca acagagcatg ttgctgctgt gtattagcct ggcagtgctg ggattcttcc
    26461 tcggaataga agtacagaaa cggcgactgg gctcgatggg ggcgctggcc agcaccataa
    26521 atattatcgt gctggataac ccgatgactt tccatttcag tcagtttctc gacagcgcat
    26581 tagggcaaat cgtcggctgt gtgctcgcgt tcaccgttat tttgctggtg cgggataaat
    26641 cgcgcgacag gactggacgt gtactgctta atcagtttgt ttctgccgct gtttccgcga
    26701 tgactaccaa tgtggcacgt cgtaaagaga accacctccc ggcactttat cagcagctgt
    26761 ttttgctgat gaataagttc ccaggggatt tgccgaaatt tcgcctggcg ctgacgatga
    26821 ttatcgccca ccagcgcctg cgtgatgcgc cgatcccggt taacgaggat ttatcggcgt
    26881 ttcaccgaca aatgcgccgc acagcagacc atgtgatatc tgcccgtagc gatgataaac
    26941 gtcgtcggta ctttggtcag ttgctggaag aactggaaat ctaccaggaa aagctatgca
    27001 tctggcaagc gccaccgcag gtgacggaac cggttcatcg gctggcgggg atgctccata
    27061 agtatcaaca tgcgttgacc gatagttaag tcaaaaccga cgccaaaagc gtcggttttt
    27121 tcatggctat acttagcgat gcacggcaga actcgccgcg aaacgtgacg gtggcaacag
    27181 atgaatattt atacctttga ttttgatgag attgagagtc aggaggattt ttatcgtgac
    27241 tttagccaaa cctttggtct ggcgaaagat aaggtacgcg atctcgactc actatgggat
    27301 gtgttaatga acgatgtcct gccgctacca cttgagattg aatttgttca tctgggagag
    27361 aaaacgcgtc gccgttttgg cgcgttaata ttgctgtttg atgaggcaga ggaagagctg
    27421 gaagggcatt tgcgttttaa tgttcgtcat tagcgcaaaa aaaagccccc gaaccggggg
    27481 caatatcgtc ggacaagacg atgagggttt atttgtacag ttcagccgta gcgtgccagg
    27541 tgtcaccgct acgagcttca gtaatctggt aggccgttgc gcctttctct tccgcttttt
    27601 tgttcagcat ttcacgcata tccattggcg aagacgccac accacttacg gatacggtcc
    27661 cgattgcttc acgattttgt gcttgtgcag catcaatgga gtcggcagcg aatgcaccga
    27721 aagagagaac agaaagtacg cttaatgcag caacagtggt tttgattttc atgattttta
    27781 cctcgacata atcttttagc tgggcctttg tttcgtgacc catatcacaa aatcaagtat
    27841 acactaatta ttga
//
LOCUS       DBFIFI010000045        27713 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.045, whole genome shotgun sequence.
ACCESSION   DBFIFI010000045 DBFIFI010000000
VERSION     DBFIFI010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27713
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.045"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(47..853)
                     /gene="trpA"
                     /locus_tag="N7B39_001829"
     CDS             complement(47..853)
                     /gene="trpA"
                     /locus_tag="N7B39_001829"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HGU2421824.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADTLELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPKKMLAALKAFVQPMKAATRS"
     gene            complement(853..2046)
                     /gene="trpB"
                     /locus_tag="N7B39_001830"
     CDS             complement(853..2046)
                     /gene="trpB"
                     /locus_tag="N7B39_001830"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HGU2421825.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(2058..3419)
                     /gene="trpCF"
                     /locus_tag="N7B39_001831"
     CDS             complement(2058..3419)
                     /gene="trpCF"
                     /locus_tag="N7B39_001831"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HGU2421826.1"
                     /translation="MMQTVLAKIVADKAIWVEARKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVICDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLKMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFADGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVLDKAKVLSLVAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(3420..5015)
                     /gene="trpD"
                     /locus_tag="N7B39_001832"
     CDS             complement(3420..5015)
                     /gene="trpD"
                     /locus_tag="N7B39_001832"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HGU2421827.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPTNTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEHLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(5015..6577)
                     /gene="trpE"
                     /locus_tag="N7B39_001833"
     CDS             complement(5015..6577)
                     /gene="trpE"
                     /locus_tag="N7B39_001833"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HGU2421828.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQSLSGNGEALLTLLDNALPAGVENEQLPNCRV
                     LRFPPVGPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTETAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLESRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(6599..6692)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(6669..6713)
                     /gene="trpL"
                     /locus_tag="N7B39_001834"
     CDS             complement(6669..6713)
                     /gene="trpL"
                     /locus_tag="N7B39_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HGU2421829.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            6851..7732
                     /gene="rnm"
                     /locus_tag="N7B39_001835"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             6851..7732
                     /gene="rnm"
                     /locus_tag="N7B39_001835"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HGU2421830.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAHIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HDGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            7729..8349
                     /gene="yciO"
                     /locus_tag="N7B39_001836"
     CDS             7729..8349
                     /gene="yciO"
                     /locus_tag="N7B39_001836"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HGU2421831.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            8377..9960
                     /locus_tag="N7B39_001837"
     CDS             8377..9960
                     /locus_tag="N7B39_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HGU2421832.1"
                     /translation="MAGKFRCILLLIVGLFFSSLSYAKNTEMPSYEEGISLFDVEATL
                     QPDGVLDIKENIHFQARNQQIKHGFYRDLPRLWMQPDGDAALLNYHIVGVTRDGIPEP
                     WHLDWHIGLMSIVVGDKQRFLPQGDYHYQIHYQVKNAFLREGDSDLLIWNVTGNHWPF
                     EIYKTRFSLKFPDIAGNPFSEIDLFTGEEGDTYRNGRILEDGRIESSDPFYREDFTVL
                     YRWPHSLLSNAPAPQTTNIFSHLLLPSTSSLLIWFPCLFLVCGWLYLWKRRPQFTSVD
                     VLRMQKFYLPRKKSSFYRPDTFLQWGGLAILAVILYGNLSPVGWAGMSLVGDMFIMIC
                     WLLPFLFCSLELLFARDDDKPCVNRVIITLFLPLICSGVAFYSLYINVGDVFFYWYMP
                     AGYFSAVFLTGYLTGMGYIFLPKFTQTGQQRYAHGEAIVNYLARKEAATHSGRRRKGE
                     TRKLDYALLGWAVSANLGREWVARITPSLTAAVRAPEIARSGVLFSLQMHLSLGANTS
                     LLGRSYSGGAAGGGGGGGW"
     gene            10173..11045
                     /gene="rluB"
                     /locus_tag="N7B39_001838"
     CDS             10173..11045
                     /gene="rluB"
                     /locus_tag="N7B39_001838"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HGU2421833.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(11085..11675)
                     /gene="cobO"
                     /locus_tag="N7B39_001839"
     CDS             complement(11085..11675)
                     /gene="cobO"
                     /locus_tag="N7B39_001839"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HGU2421834.1"
                     /translation="MSDERYQQRQQRVKEKVDARMAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDLVLLDELTYMVAYDYLPLEEVLQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPVKHAFDAGVKAQIGIDY"
     gene            complement(11672..12430)
                     /gene="yciK"
                     /locus_tag="N7B39_001840"
     CDS             complement(11672..12430)
                     /gene="yciK"
                     /locus_tag="N7B39_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HGU2421835.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCRSEDCQQLAQRIAVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVIQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            12650..13699
                     /gene="sohB"
                     /locus_tag="N7B39_001841"
     CDS             12650..13699
                     /gene="sohB"
                     /locus_tag="N7B39_001841"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HGU2421836.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVSTDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(13735..13986)
                     /gene="yciN"
                     /locus_tag="N7B39_001842"
     CDS             complement(13735..13986)
                     /gene="yciN"
                     /locus_tag="N7B39_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HGU2421837.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            14366..16963
                     /gene="topA"
                     /locus_tag="N7B39_001843"
     CDS             14366..16963
                     /gene="topA"
                     /locus_tag="N7B39_001843"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HGU2421838.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            17173..18147
                     /gene="cysB"
                     /locus_tag="N7B39_001844"
     CDS             17173..18147
                     /gene="cysB"
                     /locus_tag="N7B39_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HGU2421839.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            18466..18606
                     /gene="ymiA"
                     /locus_tag="N7B39_001845"
     CDS             18466..18606
                     /gene="ymiA"
                     /locus_tag="N7B39_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HGU2421840.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
     gene            18609..18776
                     /gene="yciX"
                     /locus_tag="N7B39_001846"
     CDS             18609..18776
                     /gene="yciX"
                     /locus_tag="N7B39_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HGU2421841.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            18890..18985
                     /gene="ymiC"
                     /locus_tag="N7B39_001847"
     CDS             18890..18985
                     /gene="ymiC"
                     /locus_tag="N7B39_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HGU2421842.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            19149..21824
                     /gene="acnA"
                     /locus_tag="N7B39_001848"
     CDS             19149..21824
                     /gene="acnA"
                     /locus_tag="N7B39_001848"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HGU2421843.1"
                     /translation="MSSTLREASKDALQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDCEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLDLDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPIYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VARSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(21888..22478)
                     /gene="ribA"
                     /locus_tag="N7B39_001849"
     CDS             complement(21888..22478)
                     /gene="ribA"
                     /locus_tag="N7B39_001849"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HGU2421844.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            22648..23412
                     /gene="pgpB"
                     /locus_tag="N7B39_001850"
     CDS             22648..23412
                     /gene="pgpB"
                     /locus_tag="N7B39_001850"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HGU2421845.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            23561..23869
                     /gene="lapA"
                     /locus_tag="N7B39_001851"
     CDS             23561..23869
                     /gene="lapA"
                     /locus_tag="N7B39_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HGU2421846.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLVRAERKIKRLENQLSPATDVAVVQHSSAAKE
                     "
     gene            23876..25045
                     /gene="lapB"
                     /locus_tag="N7B39_001852"
     CDS             23876..25045
                     /gene="lapB"
                     /locus_tag="N7B39_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HGU2421847.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            25238..25975
                     /gene="pyrF"
                     /locus_tag="N7B39_001853"
     CDS             25238..25975
                     /gene="pyrF"
                     /locus_tag="N7B39_001853"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HGU2421848.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDAALAFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            25975..26301
                     /gene="yciH"
                     /locus_tag="N7B39_001854"
     CDS             25975..26301
                     /gene="yciH"
                     /locus_tag="N7B39_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HGU2421849.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRSKGDGVVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(26427..26624)
                     /gene="osmB"
                     /locus_tag="N7B39_001855"
     CDS             complement(26427..26624)
                     /gene="osmB"
                     /locus_tag="N7B39_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HGU2421850.1"
                     /translation="MTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALGGAVLTDG
                     STLGTLGGAAVGGVIGHQVGK"
     gene            complement(26913..27662)
                     /gene="yciT"
                     /locus_tag="N7B39_001856"
     CDS             complement(26913..27662)
                     /gene="yciT"
                     /locus_tag="N7B39_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HGU2421851.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
BASE COUNT         6773 a         6762 c         7305 g         6873 t
ORIGIN      
        1 gttaaagaaa gttaaaatgc cgccagcgga actggcggct gtgggattaa ctgcgcgtcg
       61 ccgctttcat cggttgtaca aaagctttca gtgccgccag cattttcttt ggctcattaa
      121 tatgttgctc gatgatttta acaatcgcag aaccagaaat cgcgcccgca gctcctgcat
      181 caatcgctgc ttttacctga tccggggcgg aaataccaaa tccctgcaat ggaggtgccg
      241 cgttgtactc tttcagcttc gcaaccagat gattgagggg taacgcggcg cggttttctg
      301 cgccggtcac gcccgctcgt gacagcaaat atgtgtaacc acgaccgtaa gaggctatct
      361 ggcgcagcag gtcgtcatcg gcatttggcg ggcagatgaa gataggtgcg acattatgac
      421 gcaacgcggc ctggcggaag ggcgcggact cttcaactgg cacatcggca accagcaccg
      481 aatcgacgcc gactttctcg cactgggcat aaaactcatc aatgcctttg ttaaacacca
      541 ggttggcata catcagcagg ccaatgggaa tggtcgggtg tttctggcga atgagtgcca
      601 gcatctcaaa gcactgcgcc ggggtcacac ctgccgcaaa ggcacgcagt gtggcgtttt
      661 gaatcgtcgg gccatctgcc agtgggtcgg agaaggggat gcctaactcc agcgtgtcag
      721 caccggcttc aattagcgta tcgataattt tcagcgactg ctcaatgccc ggatcaccga
      781 gcgtgacgaa aggaacgaat gcgccttctt tgcgctcctt caactgggca aacagagatt
      841 cgtagcgttc catcagattt cccctcgtgc tttcaaaata tcgtgaacgg tgaagatgtc
      901 tttatcgccg cgaccggaaa ggttaaccac cagtagctgc tctttttccg ggttttcgcg
      961 catcattttc agcgcatggg ccagggcgtg ggaggattcc agcgccggga taatcccttc
     1021 gtgcaggcac agcgttttga aggcttccag tgcttcatca tcggtaattg acacataatc
     1081 agcgcgtcca gtgctgttaa gatacgcgtg ttgtgggccg acggacggaa aatcgagtcc
     1141 ggcggagatg gagtaagatt cttcaatctg cccgtcttcg gtttgcatca tcggcgcttt
     1201 cataccgaaa tagataccca cgcgaccatg ttttagcggt gcgccgtgct cgccagtttc
     1261 gataccgtga ccaccaggct ccacaccaat caggccgacg ttggtttcgt tgatgaaatc
     1321 tgcaaacatg ccgatggcat tcgaaccgcc gccaacacag gcgataacgg catccggcag
     1381 gcgaccttct ctttccagaa tctgtgcttt ggtttcttcg ccaatcatcc gctgaaactc
     1441 acggacaatg gtcggataag gatgtgggcc agctgcggtg cccagcatat agtgcgcggt
     1501 ttcataactg ccggaccagt cgcgcagcgc ctcgttacag gcatctttca gcgtcgcgga
     1561 accgctatgc accgggatca cttccgcacc cattaaacgc atacgaaaaa cgttaggcga
     1621 ctggcgttca acgtctttgg cccccatata aatacggcat ttcaggccga gcagggcgct
     1681 ggcaagggcc gacgccacgc catgctgacc ggccccggtt tcggcgatga tttcggtttt
     1741 acccatccgc ttcgccagta aagcctgacc gagcacctgg ttagttttat gcgcgccgcc
     1801 gtgcagcaaa tcttcgcgct tcagatacag cgtggtgttc gtgccagccg taatgttctg
     1861 gcatttggtc agcgcggttg gacgcccggc atagtttttc agcaggtcgt tgaactgagc
     1921 ctgaaattca ggatcttttt gcgcgctgac aaaagcttct tccagctggc gcagagcagg
     1981 catcaggatt tgtggcacgt acatgccgcc aaactcacca aaataggggt taagtaatgt
     2041 tgtcattgtt cctttcctta atatgcgcgc agcgtctgga aaaccgaggc caaaagacgt
     2101 gcgtctttga tgcccggttg cgactctaca gcagaattaa aatcaagtcc ggcgcagccg
     2161 gtttgtgccg cttccacgca gttatctgcg cctaagcccc ccgccagcag aacgttgcca
     2221 agcgattgac catttaatag tgaccagtcg aaacgttgcc cgcttccacc ctggccgttg
     2281 tctaaaacat atttatcaac gtgctgaagc tcgcgggcgg gcagggtttc accgacgctt
     2341 aatgctttcc agatggcaac gtgtgctggc agagcttcac gcagcgtatc gatatacagc
     2401 tgatcttcat taccatgcag ttgcactgcc accagtgata acaccttagc tttgtccagc
     2461 acatcggcaa tatcgtgatt gcggaacacg ccaacatact gcaacggtgc cgcagccatc
     2521 acttcctgcg cctgttcaac gttgacgcaa cgcggtgatg tcgcaacaaa aatcaaccca
     2581 ccgtaaatcg cgcccgcgtc gtacgctgct ttagcatctt gcccacgcgt caggccacat
     2641 actttattct cacccagcaa cacccgacgc acggcggcgt gcaaatcgtc atgggccatc
     2701 aacgccgaac caattagaaa accgtcagcg aagtggctta actcgcgcac ctgagcgtaa
     2761 gtattgatgc ctgattcgct gattaccgtc acgttgtgcc ccagtttcgg cgcaagctcg
     2821 cgggtacggt tgagatcaat cgacaaatca cgcagatcgc ggttgttgat gccaacgacc
     2881 tttgccccca atgcaatggc gcgctccagt tcctcttcat tactgacttc ggtcagcaca
     2941 cccatcttca gactgtgggc gacggcggca agctggcgat attgttcgtc atccagtact
     3001 gaaagcatta ataagcaggc atcggcctgg taatagcgcg ccagatagat ctggtaaggg
     3061 tcgataatga agtctttaca taaaatcggc tgcggggcga tttggctgac gatggggagg
     3121 aaatcaaagc tcccctgaaa atatttctca tcagtcagca ccgaaattgc cgaagcgtaa
     3181 tgtttataaa tggcggcaat gcgtgctgga tcgaaatcat cacagatcac gccttttgac
     3241 ggcgacgctt tcttgcactc cagaataaac gccgtgcgcg caccctgtag cgcatcataa
     3301 aaatgtcgcg tgctcggctg aacctcattc tgaaaactgg ccagcggttg ctgctgtttg
     3361 cgggcttcta cccaaatcgc cttgtctgcg acgattttcg ctaaaacggt ttgcatcatt
     3421 taccctcgtg ccgccagtgc ggtgactctg tcgtaagcgg aaccactgcg cagtacctca
     3481 agaacggttt gcgcattggc ttgcagatct tcatggccat gcaggcgcat taacatggcg
     3541 acattcgccg cgacggctgc ttcatgggcg gcgtcgcctt taccttgtaa caagcgtgtt
     3601 aaaatgtcac ggttttcttc cggtgttccg cctgccagtt gctcctggtg gtagggagtc
     3661 aggccaaaat cttcagcggt caattgatag ctcttaattt cgccgtcatg cagctcggca
     3721 acgattgtcg gcgcgtgtaa tgaaacttca tccatcccgc cgctgtgcac caccgccgcg
     3781 cgttgatacc ccagcacgcg taaggtttcg gcaatcggca gcaccagttc cggactataa
     3841 acaccaatta acgccagcgg cggatgcgcc gggttaatca atggccccag cacattgaac
     3901 agggtgcggg tttttagttg ctggcgaacc ggcattgcat ggcgaaatcc ggtgtgatat
     3961 ttcggtgcaa agaggaaaca tacacctaac tcatccagcg cctggcgcga tttatcggcg
     4021 ttcatatcaa gattaatacc gaacgccgcc agcagatccg acgagccaga tttactggag
     4081 acgctacggt tgccgtgttt cgccactttc agcccacagg ccgcggcgac aaacgcactg
     4141 gcggtggaaa tattgatgct gttgctgccg tcaccgccgg tgccgacgat atcggcaaac
     4201 agataatccg gacgcgggaa cggcgcggcg ttttccagta gcgcggttgc tgctccggcg
     4261 atctcgttcg ggtgctcgcc gcgaattttc atgctcacca gcgccgccgc caggtgttcc
     4321 ggtttcagtt caccacgtac caccgctgaa aatagctgat gactttcctg ctggctaagg
     4381 gtctgcgcct gatacagttt ttccagaatc ggttgcagcg tgttggttgg ctctagtttc
     4441 tgctgcgccc aggccagcgt ttgttccagc aggcgagcgc cctgggtggt gagaatggat
     4501 tccgggtgga actggaatcc gcaaacgcga tctgcatcgt gacgcaccgc catcaccatg
     4561 ccattaaaat gggcgttgat ggttaaaccg gccggaatat tactgccaac cagcgagtga
     4621 taacgcgcca ccggcagcgg gtttgttaat ccggcaaaca tcgcctgacc gtcatgttca
     4681 atgctcgacg ctttaccgtg aagaatttcg cccgcctgac cgacatagcc cccgtaagct
     4741 tcgacaatcg cctgatgacc aaggcaaatg ccaataatag gcagcttacc gcgcaggcgg
     4801 gtaagcagtt caggcataca accagcttcg ctcggcacac cgggtccagg agaaagcatc
     4861 agcaccggat tgctcatcgt cgcaaggcgt tcaattaagg tctgcgccgg aatatggttg
     4921 cggtaaatca ccacgttatg accattgctg cgcaactgat ctgccaggtt gtaagtaaaa
     4981 gagtcgatat tatcgagcag cagaatgtca gccattagaa cgtctcctgt gcatgatgcg
     5041 cggtggcaat agcgcgcagt acagcgcggg ctttattacg ggtttcgtcg gcttccgact
     5101 gcggaacaga atcaaggact acgccagcac cggcttgcac ggtagcgata ccgttttcca
     5161 ccagcgccga gcggatcaca atgcaggtgt cgagatcgcc gtgcgcggtg aaataaccta
     5221 ccgcgccgcc gtagctgccg cggcgacgac cttcagcctc ggcaattaac tgcatagcgc
     5281 gtactttcgg cgcaccgctt aacgtcccca tattcataca ggcgcggtaa gcgtgcaggg
     5341 cgtcgagatc gtggcgcagc tcaccgacca cgcgggagac caggtgcatc acgtaagagt
     5401 aacggtcaac tttggtgagg tcggcgacgt agcggctgcc gggggtgcaa atgcgtgcca
     5461 ggtcattacg ggcgagatcc accaacatca gatgttcaga aagctcttta tgatcggtac
     5521 gcatttccag ttcaatacgg ctttcaaggt ccctgtcgag cgagccatcg gcgcgacgac
     5581 cgcgtggacg tgttccggca atcgggtaga tctcaatctg gcggctggtg gcgtcatact
     5641 tgagcgaact ttccggcgac gcgccaaaca gggtgaaatc attatcctgc ataaaaaaca
     5701 tgtacgggct gggattactc tttttcagca cgtaataggc cgccagcggt gacgggcagg
     5761 gcagagagaa acggcgagat ggcaccacct ggaaaatttc tccggcgcga atcgcttttt
     5821 gcaacaaacg cactacgcca ccgaactctt catcgctctg gttacattca caacgcatat
     5881 gcggcacgga aaccaccggc agcggtggcg cggtttcggt cagttgctgg cgaagatcgt
     5941 tcaggcgagc agtgagacgt tgtttttctt cttcattcgg agcaaacagg ctggcctgaa
     6001 tgcgggtgct ttttttctga tggtcaatca ccatcagagt ttcagcgaga taaaaacaga
     6061 aatcagggca gttattttcc gctgacagtt gcggtaaatc ttcaaacccc gccacaaggt
     6121 cataagagaa caggccgccg aagaacatgg cttctcgttc ttccttcggt acattcaaca
     6181 gattctgcaa taagcggaaa gcgtcaaaaa ccgaaaggga gcataagcga gcgtcttcat
     6241 ccagcagtgg accaacaggg gggaagcgca gcacacggca gtttggtaat tgttcatttt
     6301 ccacacccgc aggcagggcg ttatccagta gtgtcagcag ggcttcgccg ttgccggaaa
     6361 gggactggat tgtgacagtg tcacctaaag ctgtaatgcg cagcgcactg tctaccagca
     6421 gcaggctttt taaatcatct ttgctgtcga tatctgcgga ttccagcagc agcgttgccg
     6481 gacgatcccc acacaattgg tgaaaaagcg cggtgggatt gtcgcgataa gcgccttcgc
     6541 aggttagcag ttcgagagtc ggtttttgtg tttgcattgt tattctctaa ttttgttcaa
     6601 aaaaaagccc gctcattagg cgggctgggt atctgattgc tttacgcatg gtgaatacac
     6661 tgcccgtttc aggaagtgcg ccaccaacct ttcagtacga aaattgcttt cattgtcgat
     6721 acccttttta cgtgaacttg cgtactagtt aactagttcg atgattgatt gtcaacagct
     6781 catttcagaa tatttgccag aaccgttatg atgtcggcgc aaaaaacatt atccagaacg
     6841 ggagtgcgcc ttgagcgaca cgaattacgc agtgatttac gacctgcaca gccataccac
     6901 agcttccgat ggctgcctga cgccagaagc attggtgcac cgtgcagtcg agatgcgcgt
     6961 cggcaccctg gcgatcaccg accatgacac cacagctgca atcgcgccag caagagagga
     7021 aatttcacgt tcgggactgg cgctgaatct tattcccggc gtggaaattt ccacggtctg
     7081 ggaaaatcat gaaattcata ttgtcgggct gaatattgat attactcatc cgctaatgtg
     7141 tgagttcctc gcgcagcaga cagaacggcg caatcagcgg gcgcagcaaa ttgccgaaag
     7201 acttgaaaaa gcgcatatac ctggggcgct ggaaggcgca caacgactgg cgcagggcgg
     7261 cgcagtgacg cgcggtcatt ttgcgcgttt ccttgttgag tgcggcaaag ccagttcaat
     7321 ggcggatgtc tttaaaaagt atctggcgcg cgggaaaacc ggatacgttc cgccacagtg
     7381 gtgtacaata gaacaagcta ttgatgtcat tcatcattct ggcggtaagg cggtgctagc
     7441 tcatcctggg cggtacaatc ttagtgctaa atggctgaaa agattggttg cgcattttgc
     7501 cgaacacgac ggcgacgcga tggaagtcgc gcagtgccag caatcgccca atgaacgtac
     7561 ccagctggcg acgcttgccc gccagcacca tttatgggca tcacaaggat ctgattttca
     7621 tcagccatgc ccgtggatcg aactgggtcg taaactctgg ctgcccgcag gcgttgaagg
     7681 cgtctggcag ctatgggaac agccgcagaa caccacagag agggaattat gagccagttt
     7741 ttttatattc atcctgataa cccgcagcaa cgtctaatca accaggcggt ggagatcgtg
     7801 cgtaaaggcg gggtgattgt ttatccaact gattccggct atgcgctcgg ctgtaaaatt
     7861 gaagataaaa acgccatgga gcgtatttgt cgtattcgcc agctgccgga cggtcacaac
     7921 tttaccctga tgtgtcgcga tctttctgaa ctctcgacct attcatttgt cgataacgtt
     7981 gcgtttcgtt taatgaaaaa caacacgccg ggcaactata ccttcatcct gaaggggacg
     8041 aaggaggtgc cacgccgtct gttgcaggaa aaacgcaaaa ccatcggtat gcgtgtgcct
     8101 tctaacccga ttgcgcaggc gttgcttgaa gcactgggcg aaccgatgct ttccacttcg
     8161 ctaatgctgc caggcagcga atttaccgaa tccgatcccg aagaaatcaa agaccgtctg
     8221 gaaaaacagg tcgatttgat tattcacggc ggctatctcg ggcagaaacc aacaacggtt
     8281 attgatctta ccgatgatac gccggtcgtg gtgcgtgaag gcgtaggtga tgtgaagcct
     8341 ttcttataac aaattgcgtt ccacggatgg aagactatgg cagggaagtt tcgctgcatt
     8401 ttgctgttga tagttgggct ttttttctct tcgttaagtt atgcgaaaaa cacggagatg
     8461 ccttcttatg aagaagggat ctcgctcttt gatgttgaag ccactctgca accggatggg
     8521 gtgctcgaca tcaaagaaaa tattcatttt caggcgcgaa atcagcagat taagcacgga
     8581 ttttatcgtg atttaccacg actatggatg cagcctgatg gggacgctgc actgctgaac
     8641 tatcatattg ttggcgtcac ccgtgatggt attcctgaac cctggcatct tgactggcat
     8701 atcgggttaa tgagtattgt cgtgggcgat aaacaacgtt tcttgcctca aggcgactat
     8761 cattatcaaa ttcattatca ggttaaaaat gctttcctgc gtgagggaga ttcagatctg
     8821 ttaatctgga acgtgactgg taaccactgg ccgtttgaaa tctataagac ccgattttca
     8881 ctcaagttcc ctgatatcgc gggtaatcca tttagcgaaa tcgatctctt tactggagaa
     8941 gagggcgaca catatcgaaa tggccgcatc cttgaggacg gaagaattga atccagcgat
     9001 ccgttttatc gtgaagattt cacggtactc taccgttggc ctcactcgtt acttagcaat
     9061 gccccggctc cacaaacgac gaatattttc agccatcttc ttttaccctc cacgtcatcg
     9121 ttgttaattt ggtttccgtg tctattcctg gtttgtggat ggttatatct ctggaagcgc
     9181 aggccgcaat ttacgtcggt agatgtttta cggatgcaga aattttatct gccgcgtaaa
     9241 aagtcttcgt tttaccggcc tgatactttt ttgcaatggg gtggactggc aatattggcg
     9301 gtcattcttt acggtaacct gagtcctgta ggctgggcag gaatgagtct ggtgggcgat
     9361 atgtttatta tgatctgctg gcttcttcct tttttatttt gttcccttga gcttttgttt
     9421 gcccgcgatg atgacaagcc ttgcgttaat cgtgtaatca tcactttgtt tttaccgctg
     9481 atttgttcag gcgtggcctt ttattctctc tatatcaatg tcggagatgt attcttttac
     9541 tggtatatgc cagcgggtta ttttagcgct gttttcctga ccggttatct cactggcatg
     9601 gggtatattt ttctgccaaa gtttacccaa actgggcagc aacgttatgc ccacggtgaa
     9661 gctatcgtta actatctcgc gcgtaaagag gcagcaacac acagtgggcg tcggcggaaa
     9721 ggggaaacac ggaaactgga ttacgcgttg ctaggttggg ctgtctcagc aaaccttgga
     9781 agagaatggg tagcacgtat caccccatca ctcacagcgg ctgttcgcgc cccggaaatt
     9841 gcccgtagtg gcgttttgtt ctcattacag atgcacctga gtctgggggc caataccagt
     9901 ttattggggc gaagttattc cggtggtgct gcgggtggcg gaggcggtgg tggctggtaa
     9961 ttaagctgat gttaatcgaa acaagcttta accataaaga tagtgtagta tgttgcgcct
    10021 caaagccagg ccagttaaaa cgtcgagtcg tttacttaag gcctgaagag ttcaaacaga
    10081 ctttactatc agtgagtcag atgtaagttc tgactcactg ttgcacaaaa gcatatcaga
    10141 cgcctgggaa ggcgacacca aaaggaagct ctatgagcga aaagctacag aaagtgctgg
    10201 cgcgtgccgg ccacggttct cgccgtgaaa tcgaatctat tattgaagcc ggtcgtgtga
    10261 gtgttgatgg caaaattgct aaactcggcg atcgcgttga agttacccct ggattgaaaa
    10321 tccgtatcga tggtcacctg atttcggtac gtgagtccgc tgaacaaatt tgtcgcgttc
    10381 tggcctatta taaaccggaa ggtgagttgt gtacccgtaa cgatccggaa gggcgtccaa
    10441 cggtgtttga ccgtctgcca aagctgcgcg gtgcacgctg gattgccgta ggtcgtctgg
    10501 acgttaacac ctgtggtctg ttgctgttca ccaccgacgg tgaactggct aaccgtttaa
    10561 tgcatccaag ccgtgaagtt gaacgtgaat atgccgtgcg tgtatttggt caggttgacg
    10621 acgcgaaact gcgtgattta agccgtggtg tgcagctgga agatggtccg gcagccttta
    10681 aaaccatcaa gttcagcggc ggcgaaggga tcaaccagtg gtacaacgtg actctgaccg
    10741 aaggccgtaa ccgtgaagtt cgtcgtctgt gggaagcggt tggcgtgcag gtaagccgcc
    10801 tgatccgtgt tcgttacggt gatatcccac tgccgaaagg tctgccacgc ggcggctgga
    10861 cggagctgga tcttgcccag actaactatc tgcgcgaact ggtggagttg ccgccagaaa
    10921 ccagctctaa agtcgctgta gaaaaagacc gtcgtcgcat gaaggcgaat cagattcgtc
    10981 gtgcggtgaa acgtcacagt caggtgagcg gcggacgtcg ttctggtggg cgtaataacg
    11041 gttaatcaac gtatgccgga cgtcatatcc ggcattttta cagattagta atcaatccct
    11101 atctgcgctt ttacgccagc atcaaacgca tgtttcacgg ggcgtaactc acttaccgta
    11161 tccgccagtt cgagtatatc ccgatgacag ccccgacccg tgataatcac cgtctgctga
    11221 tgtggacgtt catttaacgc ctgcaacact tcttccagtg gcaaatagtc atacgccacc
    11281 atataagtca gctcatcaag caaaaccaaa tccagtgagg aatcagcaag catccgcttt
    11341 gcatgttgcc agacttcgcg gcaggcggcg gtatcagact cgcggttttg tgtatcccag
    11401 gtaaagcccg ttgccatcac ctgaaactca acgccatgtg gctccagcag attgcgttcg
    11461 ccattaggcc aggtgccttt aataaactgc acgacgccca ctttttttcc gtgaccaact
    11521 gcgcgtgttg ccgtaccaaa tgccgcggtg gttttgcctt ttccattgcc ggtaaagacg
    11581 atgataatac cgcgttcatc ctgggcctgg gccatacgag catctacttt ttctttcact
    11641 cgctgctgac gctgttggta gcgttcatca ctcattggga aattcctggt ttacggcccg
    11701 gttgggcgtc aaaggtcatg ccggttttac ggcggctgtc atcgcccatc agccagaggt
    11761 agagcggcat gatatcagcg ggtgttttaa gtttctgtgg atcttcggtc gggaaggcgc
    11821 tggcacgcat tgcggtgcgc gtaccgcctg ggttaatgca gttgacacgc aggcgctgct
    11881 gatattcatc ggccagtacc tgcatcatcc cttcggtggc aaatttcgac gctgcatatg
    11941 caccccagtt ggctcgtccc tgacgtccaa cgcttgatga agtaaagacc agtgaaccgg
    12001 cgtccgattt gagtaataaa ggaagtagtg cctgggtgag cataaaggtg gcattaacgt
    12061 tgacctgtat gacgtcctgc cagacctgtg gattttgttc gctcattgga caaacatcgc
    12121 cgagtaatcc ggcattatgc aaaacaccat ccagacgcgg ataattaacg gcaatgcgct
    12181 gtgccagttg ttggcaatct tcggacctgc aggtcagcaa atcgagaata aaccactgtg
    12241 gctgacgccc agtttcttcg tttatgtggc tggctacctg acgtaatttt tcttcattac
    12301 ggcccaacag aatcactgtc gcaccatagc gtgcatacgt catcgcggct tcacgaccaa
    12361 taccatcact ggctcccgtc accaggataa tgcgatcatt gagtaaatct tgttttggct
    12421 ggtaatgcat ggctactcct caacgacgtt gtctgtcgcg ctgccagtgt actttatgac
    12481 tttggggctt tatgcctgaa acagaagggc atttcaatca gcctcgggcg ataacaccgc
    12541 aaaatggata ctttgtcata ctttcgctgc aataacatct ctgcgagacg gcttaacatg
    12601 cctgttgtaa actgtgagcc aaagcgttgt ttaaccaagg tggggactcg tggaattgtt
    12661 gtctgaatat ggtttgtttt tggcgaaaat cgttaccgtt gtgctagcga ttgcggcgat
    12721 tgccgccatt attgtcaatg ttgctcaacg taataaacgc cagcgtggcg agttacgggt
    12781 caacaatctc agcgaacagt ataaggagat gaaagaagaa ctggccgcgg cgctgatgga
    12841 ctcacatcag caaaaacagt ggcacaaagc gcagaagaaa aagcacaagc aagaagcgaa
    12901 agcagcaaaa gcgaaagcca aactgggcga ggtgtcaact gacagtaaac cccgcgtctg
    12961 ggtgctggac tttaaaggca gcatggacgc ccatgaagtg aactcgctac gtgaagagat
    13021 aacggcggta cttgcagcat tcaaaccgca ggatcaggtt gtgctccgtc tggaaagccc
    13081 tggtggcatg gtgcatggtt acgggttggc ggcttcgcag ctgcagcgtc tgcgtgataa
    13141 aaacattcca ttaactgtta cggtagacaa agttgctgcc agcggtggtt acatgatggc
    13201 ctgtgtggcg gacaaaattg tttccgcacc gtttgctatt gtgggttcca ttggggtggt
    13261 ggcgcaaatg cccaacttta accgcttcct gaaaagcaaa gatattgata tcgaactgca
    13321 caccgccggg cagtataagc gtacgctgac cttgctgggt gaaaataccg aagaagggcg
    13381 ggagaaattc cgcgaagagc tgaacgaaac gcatcagtta tttaaagatt ttgtgaagcg
    13441 tatgcgtccg tctctggata ttgaacaggt ggcaacgggt gaacactggt acggacaaca
    13501 ggcggtagag aagggcctgg ttgatgaaat caacaccagt gatgaagtta ttcttagcct
    13561 gatggaaggc cgagaagtgg tcaatgtacg ctatatgcag cgtaaacgac tcattgaccg
    13621 attcaccggc agcgcggcag agagcgccga tcgattattg ctacgctggt ggcagcgtgg
    13681 gcaaaagcca ttgatgtaaa agacaaacgc gagggtaaga cctcgcgttt tgctttaatc
    13741 aaccagatga tatttttctg aaagcacatg ggccaggtgt ttgaacatat taaacaccgc
    13801 ggtgcttttg gctgttggca atccttgttc atctaaaaag tagtcgccgg taaataccag
    13861 cacgccatta cgctgtgtga cgccggtggc ttcaatccct gcgagcgtat cctcatgctc
    13921 acgaatgatt ttgttggcct ctttcaacag cgtttcgcgg tcgatgggtt gtgtctcttt
    13981 gttcattatt tactccttaa acaaggacat tagtctacgc caggcatggc ttgcagacaa
    14041 atataccacg ctggtggcaa gagcgcctta ctggcaactt tggattttgc atgctaataa
    14101 agttgcgtat cggattttat caggtacagt gtgacgcttt cgtcaatctg gcaatagatt
    14161 tgcttgacat tcgaccaaaa tttcgtcgtg ctatagcgcc tgtaggccaa gacctgttaa
    14221 ctcagtcacc tgaattttcg tgaacagagt cacgacaagg ggttgatatc cgcagagagc
    14281 gagtccatat cagtaactcg ttgccagtgg aaggtttatc aacgtgcgac gcattcctgg
    14341 aagaatcaaa ttaggtaagg tgaatatggg taaagctctt gtcatcgttg agtccccggc
    14401 aaaagccaaa acgatcaaca agtatctggg tagtgactac gtggtgaaat ccagcgtcgg
    14461 tcacatccgc gatttgccga ccagtggctc agctgccaaa aagagtgccg actctacctc
    14521 caccaagacg gctaaaaagc ctaaaaagga tgaacgtggc gctctcgtca accgtatggg
    14581 ggttgacccg tggcacaatt gggaggcgca ctatgaagtg ttgcctggta aagagaaggt
    14641 cgtctctgaa ctgaaacaac tggctgaaaa agccgaccac atctatctcg caaccgacct
    14701 tgaccgcgaa ggggaagcca ttgcatggca cctgcgggaa gtgattggtg gtgatgatgc
    14761 gcgctatagc cgagtggtgt ttaacgaaat tactaaaaac gcgatccgcc aggcatttaa
    14821 caaaccgggt gagctgaata ttgatcgtgt taatgcccag caggcgcgtc gctttatgga
    14881 ccgcgtggtg gggtatatgg tttcgccgct gctatggaaa aagatcgctc gtggtctgtc
    14941 tgccggtcgt gtgcagtcgg tggcagtccg cctggtggtc gagcgtgagc gtgaaattaa
    15001 agcgttcgtg ccggaagagt tctgggaagt cgatgccagc acgaccacgc catctggtga
    15061 agcgttggcg ttgcaggtga ctcatcagaa cgacaaaccg ttccgtccgg tcaacaaaga
    15121 acaaactcag gctgcggtaa gtctgctgga aaaagcgcgc tacagcgtgc tggaacgtga
    15181 agacaaaccg acaaccagta aacctggcgc tccttttatt acctctacgc tgcaacaagc
    15241 tgccagcacc cgtcttggat ttggcgtgaa aaaaaccatg atgatggcgc agcgtttgta
    15301 tgaagcaggc tatatcactt acatgcgtac cgactccact aacctgagtc aggacgcggt
    15361 aaatatggtt cgcggttata tcagcgataa ttttggtaag aaatatctgc cggaaagtcc
    15421 gaatcagtac gccagcaaag aaaactcaca ggaagcgcac gaagcgattc gtccttctga
    15481 cgtcaatgtg atggcggaat cgctgaagga tatggaagca gatgcgcaga aactgtacca
    15541 gttaatctgg cgtcagttcg ttgcctgcca gatgacccca gcgaaatatg actccacgac
    15601 gctgaccgtt ggtgcgggcg atttccgcct gaaagcacgc ggtcgtattt tgcgctttga
    15661 tggctggacg aaagtgatgc ctgcactgcg taaaggcgat gaagatcgta tcttacctgc
    15721 agtcgataaa ggcgatgctc tgacgctcgt tgaactgaca ccagcccagc actttaccaa
    15781 gccgccagcc cgtttcagtg aagcatcgct ggttaaagaa ctggaaaaac gtggtatcgg
    15841 tcgtccgtct acctatgcgt cgatcatttc gaccattcag gatcgtggct atgtgcgagt
    15901 agaaaatcgt cgtttctatg cggaaaaaat gggcgaaatc gtcaccgatc gcctggaaga
    15961 gaatttccgc gagttaatga actacgactt caccgcgcag atggaaaaca gccttgacca
    16021 ggtggcaaat cacgaagcag agtggaaagc tgtactggat cacttcttct cggatttcac
    16081 tcagcagtta gataaagctg aaaaagatcc ggaagagggg ggtatgcgtc cgaaccagat
    16141 ggttctgacc agcatcgact gcccgacctg tggtcgcaaa atggggattc gcacagcgag
    16201 caccggggta ttccttggct gttctggcta tgcgctgccg ccgaaagagc gttgcaaaac
    16261 aaccattaac ctggtgccgg aaaacgaagt gctgaacgtg ctggaaggcg aagacgctga
    16321 aaccaacgcg ctgcgcgcaa aacgtcgttg ccccaaatgc ggcacggcga tggacagcta
    16381 tctcatcgat ccgaaacgta agttgcatgt ctgtggtaat aacccaacct gcgacggtta
    16441 cgagatcgaa gagggcgaat tccgcattaa aggttatgac ggcccgatcg ttgagtgtga
    16501 aaaatgtggt tctgaaatgc acctgaaaat ggggcgtttc ggtaaatata tggcctgcac
    16561 caacgaagag tgtaaaaaca cgcgtaagat tttacgtaac ggcgaagtgg ctccaccgaa
    16621 agaagatccg gtaccattac cggagctgcc gtgcgaaaaa tcagatgcct atttcgtgct
    16681 gcgtgacggt gctgccggtg tgttcctggc ggccaatacc ttcccgaaat cgcgtgaaac
    16741 gcgtgcgccg ctggtggaag agctgtatcg cttccgcgat cgtctgccgg aaaaactgcg
    16801 ttatctggcc gatgcgccgc agcaggatcc ggaaggtaat aagactatgg ttcgctttag
    16861 ccgtaaaacc aaacagcaat atgtctcttc ggaaaaagac ggaaaggcga ctggctggtc
    16921 agcattttat gttgatggca aatgggttga agggaaaaaa taacctttaa ttctgtcagg
    16981 tttttataaa caaagggtcg cgaaagcggc ccttttttat tgcatattat tttttcttca
    17041 cacctataca ctaaggctat aaatgatata gtggttatag ttagcacctt ttttattatt
    17101 aaatcgtatt agtcacccgc caggtgtgac gaaaaaacga tgttctgatg gcgtctaagt
    17161 ggatggttta acatgaaatt acaacaactt cgctatattg ttgaggtggt caatcataac
    17221 ctgaatgtct catcaacagc ggaaggactt tacacatcac aacccgggat cagtaaacaa
    17281 gtcagaatgc tggaagacga gctaggcatt caaatttttt cccgaagcgg caagcacctg
    17341 acgcaggtaa cgccagcagg acaagaaata attcgtatcg ctcgcgaagt cctgtcgaaa
    17401 gttgatgcca taaaatcggt tgccggtgag cacacctggc cggataaagg ctcgctgtat
    17461 atcgccacca cgcataccca ggcacgctac gcgttaccaa acgtcatcaa aggctttatt
    17521 gagcgttatc cccgcgtttc tttgcatatg caccagggtt cgccgacaca aattgctgat
    17581 gccgtctcta aaggtaatgc agatttcgct atcgccacag aagcgctgca tctgtatgaa
    17641 gatttagtga tgttaccgtg ctaccactgg aatcgggcta ttgtagtcac tccggatcac
    17701 ccgctggcag gcaaaaaagc cattaccatt gaagaactgg cgcaatatcc gttggtgaca
    17761 tataccttcg gctttactgg acgttcagaa ctggatactg cctttaatcg cgcagggtta
    17821 acgccgcgta tcgttttcac ggcaacggat gctgacgtca ttaaaactta cgtccggtta
    17881 gggctggggg taggggtcat tgccagcatg gcggtggatc cggtcgccga tccggacctt
    17941 gtgcgtgttg atgctcacga tatcttcagc cacagtacaa ccaaaattgg ttttcgccgt
    18001 agtacttttt tgcgcagtta tatgtatgat ttcattcagc gttttgcacc gcatttaacg
    18061 cgtgatgtcg ttgatgcggc tgtcgcattg cgctccaatg aagaaattga ggtcatgttt
    18121 aaagatataa aactgccgga aaaataattt cttgcgttat tttcggcacc ttttatgtag
    18181 cgaaggtgcc ggaatatatt ctcttttgtt acttatttgt ataccgtgca caatattttt
    18241 atccctcata tttatagggt aaattacctg taaagtagtg tctaattacc cgcctcgcaa
    18301 attccagttt tcaaatatct atttcaggtc aaaagattga attattcact ggagacgatt
    18361 cgtaaattcg ctggattttt gactaaagtt tctttaggat ttgtctcatc gtatgattaa
    18421 ttacaccaat cgttcaacgt cttgctgata agtgatggcg attatatgag gttagcaatg
    18481 ccttctggaa atcaggaacc ccgccgcgat cctgaattaa aacgtaaagc ctggctggcg
    18541 gtttttcttg gttctgcact tttctgggtg gttgtcgcac tgctgatttg gaaagtgtgg
    18601 ggataactat ggttggtcag gagcaactgg agtcgtcacc attatgccag catagtgaca
    18661 atgaacccga aacgaaacgg gaatgttccg tcgttattcc agacgactgg caactaacat
    18721 cgcagcagca agcctttata gaactgtttg ctgaagatga tcagccgaaa caataattat
    18781 catcattatt attacccatt tttaatgaat taaagggctt ttaatacacc gcagcaataa
    18841 caggttgagt tatctcaaca caaagtaata accgttaagg gtgtagccta tgatcaacac
    18901 aaatatgaaa tattggtcct ggatgggtgc attttctctg tcgatgctct tctgggccga
    18961 actcctctgg atcattactc actgatcctt gaccccgctg cggcggggtt gtcatttgct
    19021 ttgccacaag gtttctcctc ttttatcaat ttgggttgtt atcaaatcgt tacgcgatgt
    19081 ttgtgttatc tttaatattc accctgaaga gaatcagggc ttcgcaaccc tgtcattaag
    19141 gaggagctat gtcgtcaacc ctacgagaag ccagtaagga cgcgttgcag gccaaagata
    19201 aaacttacca ctactacagc ctgccgcttg ctgccaaatc actgggcgat atcacccgtc
    19261 tacccaagtc actcaaagtt ttgctcgaaa atctgctgcg ctggcaggat ggtaactcgg
    19321 ttaccgaaga ggatatccac gcgctggcgg gatggctgaa aaatgcccat gctgaccgtg
    19381 aaattgccta ccgcccggca agggtgctga tgcaggactt taccggcgta cctgccgttg
    19441 ttgatctggc ggcaatgcgc gaagcggtta aacgcctcgg tggcgatact gcaaaggtta
    19501 acccgctctc accggtcgac ctggtcattg accactcggt gaccgtcgat cgttttggtg
    19561 atgatgaggc atttgaagaa aacgtacgcc tggaaatgga gcgcaaccac gaacgttatg
    19621 tgttcctgaa atggggaaag caagcgttca gtcggtttag cgtcgtgccg ccaggcacag
    19681 gcatttgcca tcaggttaac ctcgaatatc tcggcaaagc agtgtggagc gaattgcagg
    19741 actgtgaatg gattgcttat ccggatacac tcgttggtac tgactcgcac accactatga
    19801 tcaacggcct tggcgtgctg gggtggggcg tgggtggcat cgaagcagaa gccgcaatgt
    19861 taggccagcc ggtttccatg cttatcccgg acgtagtggg ctttaaactt accggaaaat
    19921 tacgtgaagg tattaccgcc acagacctgg ttctcactgt tacccaaatg ctgcgcaaac
    19981 atggcgtggt ggggaaattc gtcgaatttt atggtgatgg tctggattca ctaccgttgg
    20041 cggatcgcgc caccattgcc aatatgtcgc cagaatatgg tgccacctgt ggcttcttcc
    20101 caatcgatgc tgtaaccctc gattacatgc gtttaagcgg gcgcagcgaa gatcaggtcg
    20161 agttggtcga aaaatatgcc aaagcgcagg gcatgtggcg taacccgggc gatgaaccga
    20221 tttttaccag tacgttagat ctggatatga atgacgttga agcgagcctg gcagggccta
    20281 aacgcccaca ggatcgcgtt gcactgcccg atgtaccaaa agcatttgcc gccagtaacg
    20341 aactggaagt gaatgccacg cataaagatc gccagccggt cgattatgtt atgaacggac
    20401 atcagtatca gttacctgat ggcgctgtgg tcattgctgc gataacctcg tgcaccaaca
    20461 cttctaaccc aagtgtgctg atggccgcag gcttgctggc gaaaaaagcc gtaactcttg
    20521 gcctcaagcg gcaaccgtgg gtcaaagcgt cgctggcacc gggttcgaaa gtcgtttctg
    20581 actatctggc aaaagcgaaa ctgacaccgt atctcgacga actggggttt aaccttgtgg
    20641 gatacggttg taccacctgt attggtaact ctgggccgct gcccgatcct atcgaaacgg
    20701 caatcaaaaa aggcgattta accgtcggtg cagtgctgtc cggcaaccgt aactttgaag
    20761 gccgtatcca tccgctggtt aaaactaact ggctggcctc gccgccgctg gtggttgcct
    20821 atgcgctggc gggaaatatg aatatcaacc tggcttctga gcctattggt catgatcgta
    20881 aaggcgatcc aatatatctg aaagatatct ggccatcggc acaagaaatt gcccgtgcgg
    20941 tagaacaagt ctccacagaa atgttccgca aagagtacgc agaagtcttt gaaggcactg
    21001 cagagtggaa agaaattaac gtcgcacgat ccgataccta cggatggcag gaggattcaa
    21061 cctatattcg tttatcgcct ttctttgatg aaatgcaggc aacgccagca cctgtggaag
    21121 atattcacgg tgcgcggatc ctcgcaatgc tgggggattc agtcaccact gaccatatct
    21181 ctccggcggg cagtattaag cccgacagcc cagcgggtcg atacctacaa ggtcggggtg
    21241 ttgagcgaaa agactttaac tcctacggtt cgcggcgtgg taaccatgaa gtgatgatgc
    21301 gcggcacctt cgccaatatt cgcatccgta atgaaatggt gcctggcgtt gaagggggga
    21361 tgacgcggca tttacctgac agcgacgtag tctctattta tgatgctgcg atgcgctata
    21421 agcaggagca aacgccgctg gcggtgattg ccgggaaaga gtatggatca ggctccagcc
    21481 gtgactgggc ggcaaaaggt ccgcgtctgc ttggtattcg tgtggtgatt gccgaatcgt
    21541 ttgaacgaat tcaccgttcg aatttaattg gcatgggcat cctgccgctg gaatttccgc
    21601 aaggcgtaac gcgtaaaacg ttagggctaa ccggggaaga gaagattgat attggcgatc
    21661 tgcaaaatct acaacccggc gcgacggttc cggtgacgct tacgcgcgcg gatggtagcc
    21721 aggaagtcgt accctgccgt tgtcgtatcg acaccgcgac ggagttgact tactaccaga
    21781 acgacggcat tttgcattat gtcattcgta atatgttgaa gtaacaagta tttgcttgcc
    21841 ggttattttg cttccggcaa gcaaattaat tacacaatgc aagagggtta tttgttcagc
    21901 aaatggccca ttttctcggc tttggtatcg agataatgtt cgttattggg gttacgacct
    21961 acaatcaatg gtacgcgttc aacaatatta atccccgctt cggtcagaat ttcgactttt
    22021 ttcgggttat tggttaacaa gcggacttca ttgacgccaa ggagtttgaa catatcagcg
    22081 caaagagtga agtcgcgctc atcagcggcg aagcctaact ggtggttagc ctctacagta
    22141 tcgtaacctt gatcctgcag tgcgtaagcg cggattttat tcagcagacc aatgttacga
    22201 ccttcctgac ggtgatacag caaaatacca cggccttcct cggcaatttg cgtcaatgcc
    22261 gcttcgagct ggaagccaca atcgcagcgc aagctgaaca gggcgtcacc ggtcagacat
    22321 tcggaatgga cgcgcgcaag taccggggta tgcccggaaa tatcgccata gactagcgcg
    22381 acatgatcgt gtccggttgc cagttcttca aatcccacca tcaggaaatc gccccatggg
    22441 gttggcagtt tggcttctgc cacacgttta agctgcatga aattctccag ataatgctgg
    22501 ttctgtattg gcttattttg ccataacgag aaggggttca cctaatcacg acgcgtcgat
    22561 cgttcacgga atggcacaaa tctgtcaatt tttcctggaa ctggcatttt cagttatgat
    22621 tgtgggactt atcaaaaagg agaggccatg cgttcgattg caagacgtac cgcagtggga
    22681 gctgcactat tgcttgttat gccagtagcc gtatggattt ctggctggcg ttggcaacct
    22741 ggagaacaaa gttggctact aaaagcggct ttttgggtta ctgaaactgt cacccagccc
    22801 tggggcgtca ttacacattt gattttattc ggctggtttc tctggtgtct gcgttttcgc
    22861 attaaggctg cctttgtatt atttgccatt ctggcggccg caatccttgt gggacaaggc
    22921 gttaaatcct ggatcaaaga caaagtccag gaaccacgac cttttgttat ctggctggaa
    22981 aaaacacatc atattccggt tgatgagttc tacactttaa agcgagcaga acgcggaaat
    23041 ctagtgaaag aacagttggc tgaagagaaa aatattccac aatatttgcg ttcacactgg
    23101 cagaaagaga cggggtttgc ctttccttcc ggtcacacga tgtttgctgc cagttgggca
    23161 ctgctggccg ttggtttgct gtggccgcgt cggcgaacgt taaccattgc tatcttgctg
    23221 gtctgggcaa cgggagtcat gggaagccgc ctgctgctcg ggatgcattg gccacgcgat
    23281 ctggtggtag ctacgttgat ttcgtgggca ctggtggcgg tggcaacgtg gcttgcgcaa
    23341 cgaatttgcg ggccattaac accacctgcg gaagaaaatc gcgaaatagc gcaacgagaa
    23401 caagaaagtt aacgctggtt gattttccga atttagccct taaatcatca acaatgcgcg
    23461 tggatgccat ttcgcagacg gcgcgaaaat ggtactttaa agggctattg cggtaagttg
    23521 accataattt attcgctcta accacataac gggaagtaat gtgaaatatt tactcatttt
    23581 cttactggtg ttagcgatct tcgtgatttc ggtcacgttg ggtgcgcaga acgatcaaca
    23641 ggtgacgttt aattatctgt tagcgcaagg ggagtaccgt atttccacat tgctggcggt
    23701 attgtttgct gcgggttttg ctatcggttg gttgatttgt ggcctgttct ggctgcgagt
    23761 tcgtgtttcc ctggtgcgcg ctgaacgtaa aataaagcga ctggaaaacc agctttcacc
    23821 cgcgactgac gtggctgtag tgcagcactc gtcagcggcg aaggaataac tttctatgct
    23881 ggagttgttg tttctgcttt tgcctgtagc cgctgcctat ggctggtata tgggccgcag
    23941 aagtgcgcaa caaaacaagc aagatgaagc caaccgcttg tcgcgtgatt acgtagcggg
    24001 ggttaacttc ctgcttagta atcaacagga taaagcggta gacctgtttc tcgatatgct
    24061 taaagaggat acaggcaccg ttgaagccca ccttacgctc ggaaacctgt tccgttcgcg
    24121 tggcgaagtt gatcgcgcta ttcgcatcca tcagacccta atggaaagcg cctcgctgac
    24181 ctatgaacag cgtctgttgg cgattcaaca actggggcgt gattacatgg ccgccgggtt
    24241 atatgaccgc gcggaagaca tgttcaatca gctgaccgat gaaactgact tccgcattgg
    24301 cgcgctgcaa cagttgctac aaatctacca ggctaccagt gagtggcaga aagcaattga
    24361 tgttgccgaa cgcctggtga agctgggtaa agataaacag cgcgtcgaaa ttgcccattt
    24421 ctactgtgag ttagccctgc agcatatggc cagcgacgat ctcgatcgtg ccatgacctt
    24481 gctaaaaaaa ggggcggcgg cagataaaaa cagcgcccgc gtatccatca tgatgggacg
    24541 cgtgtttatg gcgaaaggag aatacgccaa agccgtcgaa agtttgcaac gcgtgatatc
    24601 ccaggacaga gaactggtca gcgaaacgct ggaaatgctg caaacctgct atcagcagtt
    24661 gggtaaaact gccgaatggg cagaattcct gcagcgcgcg gtggaagaga acaccggtgc
    24721 cgatgctgaa ttgatgcttg ctgatatcat cgaagcgcgc gacggtagtg aggccgcaca
    24781 ggtctatatt acgcgccagc ttcagcgtca tccgaccatg cgtgtgttcc ataagttaat
    24841 ggattaccac ttaaatgaag cggaagaagg gcgtgccaaa gagagcctga tggtgctgcg
    24901 tgacatggtt ggcgagaagg tacgtagtaa gcctcgttat cgctgccaga aatgtggttt
    24961 taccgcatac accctctact ggcattgtcc gtcttgtcgt gcctggtcaa ctattaaacc
    25021 gattcgcggt cttgatggcc tgtaattttt taaaaaaacc gactttagtt acaacatact
    25081 agttatttat gtcccattgt gctccggcaa cggcggcgca gaaaaagcct gccaggggag
    25141 aaatcgcaac tgttaatttt ttatttccac gggtagaatg ctcgccgttt acctgtttcg
    25201 cgccacttcc ggtgcccatc atcaagaagg tctggtcatg acgttaactg cttcatcttc
    25261 ttcccgcgct gttacgaatt ctcctgtggt tgttgccctt gattatcata atcgtgatgc
    25321 cgcgctggcc tttgtcgaca agatcgaccc acgcgattgt cgtctgaagg tcggcaaaga
    25381 gatgtttaca ttgtttgggc cacagtttgt gcgcgaactt caacagcgtg gttttgatat
    25441 ctttcttgac ctgaaattcc acgatatccc caacactgca gcgcacgctg tcgctgctgc
    25501 agctgactta ggcgtgtgga tggtgaatgt tcatgcctct ggtggggcgc gtatgatgac
    25561 cgcagcgcgt gaggcactgg ttccgtttgg caaagatgca ccgcttttga ttgctgtgac
    25621 agtgttgacc agcatggaag ccagcgacct ggccgatctt ggcgtgacac tgtcacctgc
    25681 agattatgca gaacgtctgg cagcactgac gcaaaaatgt ggccttgatg gtgtggtgtg
    25741 ttctgctcag gaagctgtgc gctttaaaca ggtattcggt caggagttca aactggttac
    25801 gccgggcatt cgtccgcagg ggagtgaagc tggtgaccag cgccgcatta tgacgccaga
    25861 acaggcgttg gcggctggtg ttgattatat ggtgattggt cgcccggtaa cgcaatcggt
    25921 agatccagcg cagacgctga aagcgatcaa cgcctcttta cagcggagtg catgatgagt
    25981 gattccaaca gccgtctggt ctactcaaca gaaaccgggc gtattgatga gcccaaagcg
    26041 gccccagtac gctctaaagg cgatggtgtg gtgcgtattc agcgtcagac cagtggacgt
    26101 aaaggtaagg gcgtttgcct gattaccggt atcgatctcg atgatgccga actgacaaaa
    26161 cttgcagcgg aactgaagaa aaaatgcggc tgcggcggag cagttaaaga tggagttatt
    26221 gaaatccagg gcgataagcg tgatttatta aaatcactac ttgaagcgaa agggatgaag
    26281 gtaaaactcg caggcggtta acataaaaag ccacggatat atccgtggct ttcgaatatt
    26341 ttactgtgtg tattattcag ttatttcgta ctgaacagaa tcaagcgtat tataattacg
    26401 agtcgataat cgacatttat ttacgattat ttaccgacct ggtgaccaat aacaccaccg
    26461 acagctgcgc cacctaatgt acccaacgta ctgccatcgg tcagtactgc accgcctaat
    26521 gcccctgcac ccgcgccgat tgcggtgttg cggtcccgtt tagaccagtt agaacaggca
    26581 ctcagagaca ttgccagggt aattgccaga acagccgcgg tcattttttg ctcgttacaa
    26641 acataatact ctctcctgaa tttatgattc acggaagtaa gctctctata actataatag
    26701 ctaagaataa gcctggtgaa attattccgt gaaatccgct cgcgcgcagg gttatatcac
    26761 gcaggtgata atgacttcct gttatatcgc tgataataat tttatatctt gagagtgtta
    26821 ataacaggta aatagtctta attatcaacc aggaatcatc ttagagcgga tgatttgtca
    26881 aactgcaaat catccgtaga gaagggaaat ggttaaatgt caacgacgtg aacggtgagt
    26941 ttactgtgct caagatgcat cagatcgctg gcgcgtattt tcgaatcggt aatcacgcga
    27001 ttgaatcgct caacgggacc gatggagtat gaatgtacag caccaaattt cgagctgtca
    27061 gtcaggacta tcgcttcgca ctctttttcc agcacggcat tcaccacatc ggtacgcatc
    27121 atatcgcgac cggtgaatcc agtttcaggt tgccagccat caataccaat aaatgctttg
    27181 ctgaaatgta cctgttggat gcactggcgt gtcaacgggc caaccatact ttcgcttttt
    27241 ttctggtaca cgccaccgag caaaataact tcacaaggcg cgtctttcag caaatgcgcg
    27301 atgtagctgc tgaccgtgat gatagtgaca tttttcttct gctcccccag agtccgggcg
    27361 agcagggcat tgctgctgcc attttcgata aagatggttt cgcccggttg aaccagtgac
    27421 gcggcaaact cggcgagttc acgcttcagc gtatagttgc tcatcatacg ggtttcgacg
    27481 tcatcactat caagcgaaac tgcaaagcca tgtgcacggc ggaggtaact cagtttttcg
    27541 agggtgttga gatcctggcg aatggtaact tcagaaactc cagtggcttt tgccagatcg
    27601 gttacgctaa cctgaccctg gtcaatgacc atctgtagaa tagtttgttg tcgggaattc
    27661 atcgctgtaa ttcttataac gttataatac taaataaaaa aatactgacg cgc
//
LOCUS       DBFIFI010000046        27518 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.046, whole genome shotgun sequence.
ACCESSION   DBFIFI010000046 DBFIFI010000000
VERSION     DBFIFI010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27518
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.046"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            2..280
                     /locus_tag="N7B39_001857"
     CDS             2..280
                     /locus_tag="N7B39_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HGU2421852.1"
                     /translation="MVIFYGIIILHDIPYLIAKKRNHPHADAIHVAGWVSLFTLHVIW
                     PFLWIWATLYRPERGWGMQSHDSSVMQLQQRIAGLEKQLADIKSSSAE"
     gene            286..1422
                     /gene="yiaV"
                     /locus_tag="N7B39_001858"
     CDS             286..1422
                     /gene="yiaV"
                     /locus_tag="N7B39_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HGU2421853.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQKAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELNEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(1419..2393)
                     /gene="yiaU"
                     /locus_tag="N7B39_001859"
     CDS             complement(1419..2393)
                     /gene="yiaU"
                     /locus_tag="N7B39_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU2421854.1"
                     /translation="MTKLQLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADF
                     ESKVGLKVFDRTTRQLMLTLFGTALLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYA
                     PTGIITYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAPPKDE
                     SLVASFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGS
                     KEVRDYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWW
                     LDLVAIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
     gene            2517..3257
                     /gene="yiaT"
                     /locus_tag="N7B39_001860"
     CDS             2517..3257
                     /gene="yiaT"
                     /locus_tag="N7B39_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HGU2421855.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNENT
                     KAIPLISYEGDSFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDYAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITDSPMIDRNESFTFVTGVSWRF"
     gene            complement(3387..5357)
                     /locus_tag="N7B39_001861"
     CDS             complement(3387..5357)
                     /locus_tag="N7B39_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HGU2421856.1"
                     /translation="MNISEVDLHKLTVSDPFLGQYQQLVRDVVIPYQWDALNDRIPEA
                     EPSHAIENFRIAAGLQEGEFYGMVFQDSDVAKWLEAVAWSLCQKPDAELEKTADEVIE
                     LIASAQCEDGYLNTYFTVKAPEERWSNLAECHELYCAGHLIEAGVAFFQATGKRRLLG
                     VVCRLADHIDSVFGPDESKLHGYPGHPEIELALMRLYEVTEEPRYLALTNYFVEQRGA
                     QPHYYDQEYEKRGQTSHWHTYGPAWMVKDKAYSQAHLPLAQQQTAIGHAVRFVYLMTG
                     VAHLARLSHDDSKRQDCLRLWNNMAQRQLYITGGIGSQSSGEAFSSDYDLPNDTVYAE
                     SCASIGLMMFARRMLEMEGDSQYADVMERALYNTVLGGMALDGKHFFYVNPLEVHPKS
                     LKFNHIYDHVKPIRQRWFGCACCPPNIARVLTSIGHYLYTPREDALYINIYAGNSMEV
                     PVENGTLRLRVSGNYPWQEQVTIAVESPQPVRHTLALRLPDWCTQPQIILNGEEVEQD
                     IRKGYLHITREWQEGDTLNLTLPMPVRRVYGNPLVRHVAGKVAIQRGPLVYCLEKADN
                     GESLHNLWLPTDAPFTTFEGKGLFSHKILIQAPGYRYEQSNPEQQPLWHYDSAPAKRQ
                     TQTLTFIPWFSWANRGEGEMRIWVNEEKHCHP"
     gene            complement(5368..6768)
                     /locus_tag="N7B39_001862"
     CDS             complement(5368..6768)
                     /locus_tag="N7B39_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2421857.1"
                     /translation="MTSTPITTADIAKGKIDDALSVREKIGYGLGDAGGTVITCLIMN
                     FLTFFYTDVFGLTPALVGTLFIALRVFDAISDPVMGVIADRTQSRWGRFRPWQLWIAI
                     PIGIIGILTFTVPDASMGVKIAWAFGTYLLLSVGYTAINVPYCARINTMTTRHNEVIS
                     CQSWRFVLCGVAGFLVSVGLPWMVALFGQGNAARGYQLGVGVLCAIAVVMFLCCFFWV
                     RERVPLSTMGKFTLREHLAGLRNNDQLLLMLVMSFLLINVFNIRGGGYMYFITYVLQG
                     STGYTSLFFTMVTFASIIGSVIVSPLTRRFDTVKIYYYTNLLLAALAVLMWFLPSGPA
                     YQTLWLAVILGNGVILGFTLPLHFSLMAFADDYGEWKTHVRSSGMNFAFNLFFIKLAW
                     ASSAGIISLLFIFVAYQPGVENQTASSLGGIAAMETLLPALFHLLLAGAIRFCKLNNP
                     MMSRIATDLRQRHVQP"
     gene            6994..7809
                     /locus_tag="N7B39_001863"
     CDS             6994..7809
                     /locus_tag="N7B39_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU2421858.1"
                     /translation="MLELSMTLPIKVQNGGLFISRGVGRHSARRLESWEIIFVEKGRL
                     TIQEEERIFLVDAGESLLLWPNRQHVGVEDFPADLKFYWLHFEVKAPDNDPRWLTHLS
                     VPQQTKVVDPQALVALFRQFLSEQEKHQRTPALEFIVLLILQQLTRDARLDEQADGAG
                     TTLAWKAQKLIRTQYHRPLSTALLARELYCNADYLGRVYRRVFHLTLTEAIHRQRVRE
                     AEKLLISEARSLTEVAALCGFNDVGYFRQIFRKHTGLTPAAWKRRYSKEHINS"
     gene            7811..7951
                     /locus_tag="N7B39_001864"
                     /pseudo
     CDS             7811..7951
                     /locus_tag="N7B39_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
     gene            8053..8526
                     /gene="ysaA"
                     /locus_tag="N7B39_001865"
     CDS             8053..8526
                     /gene="ysaA"
                     /locus_tag="N7B39_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HGU2421859.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(8678..9931)
                     /gene="avtA"
                     /locus_tag="N7B39_001866"
     CDS             complement(8678..9931)
                     /gene="avtA"
                     /locus_tag="N7B39_001866"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HGU2421860.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEAQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(10109..12139)
                     /gene="malS"
                     /locus_tag="N7B39_001867"
     CDS             complement(10109..12139)
                     /gene="malS"
                     /locus_tag="N7B39_001867"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HGU2421861.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDASAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKAIDGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCIAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            12459..13283
                     /gene="bax"
                     /locus_tag="N7B39_001868"
     CDS             12459..13283
                     /gene="bax"
                     /locus_tag="N7B39_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HGU2421862.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTSRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            complement(13391..14569)
                     /gene="xylR"
                     /locus_tag="N7B39_001869"
     CDS             complement(13391..14569)
                     /gene="xylR"
                     /locus_tag="N7B39_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
                     /protein_id="HGU2421863.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(14647..15828)
                     /gene="xylH"
                     /locus_tag="N7B39_001870"
     CDS             complement(14647..15828)
                     /gene="xylH"
                     /locus_tag="N7B39_001870"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HGU2421864.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            complement(15806..17347)
                     /gene="xylG"
                     /locus_tag="N7B39_001871"
     CDS             complement(15806..17347)
                     /gene="xylG"
                     /locus_tag="N7B39_001871"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HGU2421865.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            complement(17425..18417)
                     /gene="xylF"
                     /locus_tag="N7B39_001872"
     CDS             complement(17425..18417)
                     /gene="xylF"
                     /locus_tag="N7B39_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU2421866.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLQPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            18783..20105
                     /gene="xylA"
                     /locus_tag="N7B39_001873"
     CDS             18783..20105
                     /gene="xylA"
                     /locus_tag="N7B39_001873"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HGU2421867.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAACMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            20177..21631
                     /gene="xylB"
                     /locus_tag="N7B39_001874"
     CDS             20177..21631
                     /gene="xylB"
                     /locus_tag="N7B39_001874"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HGU2421868.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECALLEARVPLSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLSNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            21800..22141
                     /gene="yiaB"
                     /locus_tag="N7B39_001875"
     CDS             21800..22141
                     /gene="yiaB"
                     /locus_tag="N7B39_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HGU2421869.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            22187..22624
                     /gene="yiaA"
                     /locus_tag="N7B39_001876"
     CDS             22187..22624
                     /gene="yiaA"
                     /locus_tag="N7B39_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HGU2421870.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(22666..23661)
                     /gene="wecH"
                     /locus_tag="N7B39_001877"
     CDS             complement(22666..23661)
                     /gene="wecH"
                     /locus_tag="N7B39_001877"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HGU2421871.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLFFYSAIALLYIALFTSINV
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRALGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYPYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            23836..24135
                     /gene="ysaB"
                     /locus_tag="N7B39_001878"
     CDS             23836..24135
                     /gene="ysaB"
                     /locus_tag="N7B39_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HGU2421872.1"
                     /translation="MMMNSFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            24230..25141
                     /gene="glyQ"
                     /locus_tag="N7B39_001879"
     CDS             24230..25141
                     /gene="glyQ"
                     /locus_tag="N7B39_001879"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HGU2421873.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            25151..27220
                     /gene="glyS"
                     /locus_tag="N7B39_001880"
     CDS             25151..27220
                     /gene="glyS"
                     /locus_tag="N7B39_001880"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HGU2421874.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPSEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRINRLTMLEKLRELFLRV
                     ADISLLQ"
BASE COUNT         6827 a         6730 c         7076 g         6885 t
ORIGIN      
        1 tctggtgata ttctatggga ttattatttt acatgatatt ccctacctga ttgctaaaaa
       61 acgtaatcat cctcatgccg acgccattca tgttgctggt tgggtgagtc tttttacgct
      121 gcatgttatc tggccatttt tgtggatttg ggccacgctt taccgcccgg agcggggatg
      181 gggaatgcaa agccatgatt catccgttat gcaactgcaa cagcgcattg ccgggctgga
      241 gaaacagctc gccgacatca aatcctcttc tgccgagtaa tacttatgga tttactgatt
      301 attttgacct atgtggcttt tgcatgggca atgtttaaga tcttcaaaat tcccgtaaat
      361 aaatggacca ttcccacagc ggccctgggt ggaatattta ttgtcagcgg tctaattctg
      421 ttaatgaact ataaccatcc gtataccttt aaagcgcaaa aagcggttat ttctattcct
      481 gttgtcccac aggtgacagg cgtggtgata gaagtgacgg ataagaaaaa tacgctgatt
      541 aaaaaaggtg aggtgctatt tcgactggac ccgacgcgtt atcaggcgcg ggtggatcgg
      601 ctgatggcgg atatcgttac cgcagaacat aaacagcggg cgttgggcgc agagttagat
      661 gagatggcgg cgaatactca gaaggcaaag gccacgcggg ataaattcgc taaggagtac
      721 cagcgttacg cacgcggcag ccaggcgaaa gtaaacccgt tttcagaacg cgatattgat
      781 gtggcacggc aaaattatct ggcgcaggaa gcctccgtaa agtcatcggc ggcggaacaa
      841 aaacagatcc agagccagct ggatagcctg gtgttgggtg aacattctca aatcgccagc
      901 ctgaaagcgc agctcgcgga agcaaaatat aaccttgagc agacgatagt gcgtgcgccg
      961 agcgatggtt atgtgaccca agtgctgatt cgtccgggta cctatgccgc gtcgctgccg
     1021 ctacgtccgg tgatggtgtt tatacccgat cagaaacgac aaatcgtggc gcagttccgt
     1081 cagaactcct tgctgcgcct ggctcccggc gatgatgcgg aagtggtatt taacgctctg
     1141 ccaggcaaag tgttcagcgg taagctggca gccattagtc cagctgttcc cggcggagct
     1201 tatcagtcga ccggcacctt acagacgtta aacacagcgc cgggttcaga tggcgttatc
     1261 gcgaccattg aactgaatga gcacactgat ttgagcgctt taccagacgg tatttacgct
     1321 caggtggcgg tctactctga tcatttcagc catgtctcgg tgatgcgcaa agtgttatta
     1381 cgcatgacca gctgggtgca ttacctttat ctcgatcatt aatcttcgct ttccgccgct
     1441 tgttcaggcc gcagttgctg cgactgagca agctggtggc gaatttcacg cagcatttca
     1501 tccagcacat actggacccg ccagggctgg tattcccgtt tacggaagat agccaccaga
     1561 tccagccacc attcatacgg gtgctgaaag cagggcacca gcgtgccgtc ttgcagatcg
     1621 ctctgcacac ttttgtctgg cgcaaacaca atccccaggt gatttcgtgc cagctccagc
     1681 gccgattgcg tgttatcgca aacgtaattt cccttcacgc gataatcccg cacctcttta
     1741 ctcccggcga cgttgaagcg ccagatattc gcgtcgtcga tcatcatcga gtcgatcaaa
     1801 atacaggagt gatgttcgag ttcgtcagga cggctaatag gatgtttctc aagatagcgc
     1861 tggctggcat aagcggttac ggcatattgg gtaataaaac tggcaaccag cgattcatct
     1921 tttggtgggg catagctgat taaaacatca caatcatcag gaaattcgac gccttcataa
     1981 aaggcattac gctcaagatt gcaggttttt agcgataagg tgatatcacc gatatcttta
     2041 attttatcga ttacatgttt ggataaatag gtaattatgc cggttggggc gtagatggtg
     2101 acccgaccac gtttctcatg cttataatct gcaataaaat tattaagctg ctcgtttctg
     2161 tccagcatgt cgttgatgta cggtaatagc gcggtgccaa aaagtgtgag catcagttgt
     2221 cgggtggttc ggtcaaagac ttttaaaccc acttttgatt caaaatcagc aagatatttg
     2281 ctgacgttgg cctgtgcgat gccaagtaca gtcgcggcat ggctgatatt ttcactggca
     2341 gcgattaccg agataatttt taactcccgg tacttaagtt gtaattttgt catcgcaaca
     2401 gcccaattat atataatttt atatattact atataaatat gaatcttgca ccggagaaat
     2461 tgtaagcatt actatgccga ctctttttta tcttatttta aaatggaata ccggacatgt
     2521 taattaaccg caatattgtg gcgttatttg cgttgccttt tatggcaagc gcaactgctt
     2581 ctgaattatc cattggtgct ggtgcggctt ataatgaatc gccttatcgc ggttataatg
     2641 aaaatacaaa ggcaattccg ctgattagtt atgaaggtga ttctttttat gttcgtcaga
     2701 ccacgttagg ttttattctg tcgcaaagtg aaaaaaatga acttagcctg accgcatcct
     2761 ggatgccgct ggaatttgac cctgccgata atgacgatta tgccatgcaa cagcttgata
     2821 agcgtgatag tacggctatg gcgggggttg cctggtatca ccacgagcgt tggggaaccg
     2881 tgaaagcctc tgcagctgcg gacgttctgg ataacagcaa cggctgggtg ggggagctat
     2941 cggtattcca caaaatgcag ataggtcgtc tgtcgctgac acctgcgctg ggcgttctct
     3001 attatgacga gaatttcagt gactattact atggcatttc agagagtgag tcccgtcgta
     3061 gcggtctggc aagttattcc gcgcaggatg cctgggtgcc ctatgtcagc ctgacggcaa
     3121 aatacccgat aggagagcac gtcgtattga tggcgagcgc aggatacagc gagctgccgg
     3181 aagagattac cgacagcccg atgattgatc gtaatgagag ttttaccttt gtcaccggcg
     3241 tgagctggcg tttttaattc accggtggat gtcggtgcgg ctggcctacg ctctcgcacc
     3301 gacttgtatg cctgataaga cgtgttaagc gtcgcatcag gcaatgtgct ccttacgttg
     3361 gatgcggcgc gagtgcctta tccaacctac ggatggcaat gcttttcctc attcacccag
     3421 atccgcattt cgccttcacc ccggttggcc cagctaaacc acgggataaa ggtcagagtt
     3481 tgcgtctggc gtttggctgg cgcgctgtcg taatgccaca gcggttgctg ctctggattg
     3541 ctctgttcat accggtaacc cggtgcctgg attaagatct tatggctaaa caatcccttg
     3601 ccttcaaatg tcgtaaatgg cgcatcggtg ggcagccaca gattatgcag tgactcgccg
     3661 ttgtcggcct tttccaggca atacaccagc gggccgcgct gaatcgccac ttttccggcg
     3721 acgtgacgca ccagcgggtt accgtaaacg cggcgtaccg gcatcggcaa agtcagattc
     3781 agcgtatcgc cctcctgcca ttcgcgggta atgtgcaaat accctttacg aatatcctgc
     3841 tcgacctctt ccccattcaa tatgatctgc ggctgtgtgc accagtccgg cagacgtaaa
     3901 gccagcgtat gacgtaccgg ctggggcgat tcaaccgcaa tcgtcacctg ctcctgccac
     3961 ggatagttcc cgctaacccg caggcgcagc gtgccatttt ctaccggcac ttccatgctg
     4021 tttcctgcgt agatgttgat atacaacgca tcttcacgcg gcgtgtagag ataatgacca
     4081 atcgaggtca gcacgcgggc gatatttggc ggacaacaag cgcagccaaa ccaacgctgg
     4141 cggatcggtt taacgtgatc gtagatatgg ttgaatttca gcgattttgg atgtacttcc
     4201 agtggattca catagaagaa atgtttgcca tccaatgcca tgccgccgag cacagtgttg
     4261 tacagtgcgc gctccatcac atcggcatat tgactgtcgc cttccatttc cagcattcgt
     4321 cgggcgaaca tcatcaggcc gatggaagca caactttcgg cgtaaaccgt gtcattcggc
     4381 agatcgtaat cgctgctgaa cgcttcgccg ctgctttgtg agccgatgcc gccggtaata
     4441 tataactgac gctgggccat attgttccac agccgcaggc agtcctgacg cttgctgtca
     4501 tcgtgactta aacgcgcgag atgcgcgacg ccggtcatca gatagacaaa acgtaccgcg
     4561 tgaccgatgg cggtttgctg ttgcgcaagg ggcaaatgtg cctggctgta ggctttgtct
     4621 ttcaccatcc acgccgggcc gtaggtgtgc cagtgcgatg tctgcccgcg cttttcatat
     4681 tcctggtcgt aatagtgcgg ttgcgcacca cgctgttcga caaaatagtt cgtcagcgcc
     4741 aggtagcgcg gctcttcagt cacttcatac aggcgcatta gtgccagttc aatttccggg
     4801 tgaccaggat aaccgtgtaa cttactttca tctggaccaa atacgctgtc gatatgatcg
     4861 gccaggcggc aaacgacccc cagcaagcgc cgttttcccg tggcctggaa gaaggcgact
     4921 ccggcttcaa tcagatgacc tgcgcagtaa agttcatgac actccgccag attgctccag
     4981 cgttcttcgg gtgcttttac cgtaaagtaa gtattgagat agccgtcttc gcattgggcg
     5041 gaggcgatca gttcgattac ctcgtcggcg gttttttcca gttcggcgtc cggcttctgg
     5101 cacagcgacc aggctaccgc ttccagccat ttggcgacgt cgctgtcctg aaacaccatc
     5161 ccgtaaaatt caccctcctg aagtccggcg gcaatgcgaa agttttcaat cgcatggctg
     5221 ggttccgctt ctgggatacg atcgttcaag gcatcccact gataaggaat caccacgtcg
     5281 cggaccagtt gttggtactg accgaggaac ggatcgctga ccgtcagttt atgcagatcg
     5341 acttccgaaa tgttcatctt gtgctcctta aggctgtaca tgacgctgac gcaggtcggt
     5401 agctatgcgt gacatcatag gattattgag tttgcaaaag cggatcgccc ctgccagcag
     5461 caggtggaat agcgcaggca gtaatgtttc catcgccgcg atcccgccaa gcgaactggc
     5521 ggtctggttt tccacgccag gctggtaggc gacaaaaata aacagcaggc tgatgatccc
     5581 ggcgctggag gcccaggcca gcttgatgaa aaacagattg aaggcgaagt tcatgccgga
     5641 agaacgtacg tgggttttcc actcgccgta gtcatcggca aaggccatca atgagaagtg
     5701 cagtggcaat gtgaagccaa gaatcacgcc attaccgagg atcaccgcca gccacagcgt
     5761 ttgataagcc gggccggagg gcaggaacca catcaacacc gccagtgcag cgaggagcag
     5821 gttggtgtag taataaattt tgacggtatc gaaacgccgc gttaacgggc tgacaatcac
     5881 cgagccgata atggaggcga aggtgaccat ggtgaagaac agcgacgtgt agcccgtgct
     5941 gccttgtaag acgtaggtaa tgaagtacat atacccaccg ccgcgaatgt taaagacgtt
     6001 aatcagcagg aaagacatga ccagcatcag cagcagttgg tcgttgttcc gcagcccggc
     6061 aagatgttcg cgcagggtaa atttccccat tgtggagagc ggcacccgtt cacgaaccca
     6121 gaagaaacag cacaggaaca tcaccacggc aatggcgcac aataccccga cgcccagttg
     6181 atagccgcgt gcagcgttgc cctgaccgaa gagagctacc atccacggta agcctaccga
     6241 aaccagaaat cccgctacgc cgcagagaac gaatcgccag gactggcagg agatcacttc
     6301 attgtggcgg gtggtcatgg tgttgatccg cgcgcagtac ggtacgttga tggcggtata
     6361 accgactgaa aggagcaggt aagtaccgaa cgcccaggcg atttttactc ccatgctggc
     6421 atctggcacg gtgaacgtca ggatgccgat aatgccgatg ggaatggcaa tccatagctg
     6481 ccacggacga aagcgccccc atcggctttg cgtccggtcg gcaatgacgc ccatcaccgg
     6541 gtcggagatg gcatcaaaca cgcgcagtgc aataaacagc gtgccaacca gcgccggagt
     6601 taatccaaaa acgtcggtgt agaaaaaggt gagaaaattc atgatcaggc aagttattac
     6661 ggtgccgcct gcgtcaccca ggccgtagcc tattttttcc cgtacagata acgcatcgtc
     6721 aattttacct ttagcgatat cagcggtagt aatcggtgta gaggtcatag atattcctcg
     6781 tgaataggga gcaattttta gttttattga aatgacctga ttaattctgc cgtgaacggg
     6841 aatatcaaca aggcgagaaa agagtaaaaa aaggtgacga ttccgacctg aatttaaaag
     6901 tgtgaaatgg atcaggtgaa attaatattt ttgattaata atcaaaaggt aatgtgttta
     6961 atcattaatg aataagcggg aaattacata tcaatgctcg aattatccat gacgttaccg
     7021 attaaagtgc aaaatggtgg attatttatt tcccgtggcg tagggaggca ttctgcaagg
     7081 cggctggagt catgggaaat tatctttgtc gaaaaaggtc gattaacgat tcaggaagaa
     7141 gaacggatat ttctggtcga tgcgggagag agtttattac tctggccgaa ccgacaacat
     7201 gttggggtgg aggattttcc cgccgatctc aaattttact ggctgcattt tgaggttaaa
     7261 gcgccggaca acgaccctcg ctggcttact catcttagcg tgccgcaaca aacgaaggtt
     7321 gtcgatccgc aggcgctggt ggccctgttt cgccagttct taagcgaaca ggaaaagcat
     7381 caacgcactc cggcgctgga gtttattgtg ctgctcattt tgcagcagct cacccgtgac
     7441 gcgcgtcttg atgaacaggc cgatggcgca ggcaccaccc ttgcctggaa agcgcagaaa
     7501 ctgatccgca cgcaatatca tcgcccgctt tctacggcgc ttctggcccg cgagctgtac
     7561 tgcaatgccg attatcttgg gcgcgtctac cggcgggtgt ttcatctgac gttaacagaa
     7621 gcaatccatc gccagcgggt gcgcgaagcc gaaaagctgt taattagcga agcgcgatcg
     7681 ctcacggaag tggcggcgtt gtgcgggttt aatgacgttg gctatttccg gcagattttt
     7741 cgcaaacaca ccggtttaac gccagcagcg tggaagcggc gttacagcaa ggaacatatc
     7801 aattcgtagt gccggggcga tttcgctttc gacatcacgt ctgaaacagg tgggggagaa
     7861 aaatctcctg aaaccactgc gtgaaaccgc gcaggctatt tctaatgaac tgggatttac
     7921 tgtcagggac gaccagggcg cgatcacata acgctttgga caagtgccaa aactttaaca
     7981 tttccttcgt tggatcaaag cagtagggac gcgctctctg gcactctgct gttttagtgc
     8041 aaaggagtga tcatgaaccg gtttattatt gcggatgcga cgaaatgtat cggttgccgt
     8101 acctgtgaag tggcttgcgc agtgtcgcat caggagaatc aggattgcgc tgcgttgtca
     8161 ccagacgagt ttatttcccg tattcgtgtc attaaagacc acagctggac cacggcagta
     8221 gcctgtcatc agtgtgaaga tgcaccgtgc gcgaatgtct gccctgttga cgcgataagc
     8281 cgcgaacatg ggcatatttt cgttgaacaa acacgttgca ttggctgtaa aagctgtatg
     8341 ctggcttgcc cgtttggtgc gatggaggtc gtttcttcgc gcaaaaaggc gagggcgatt
     8401 aagtgcgatc tgtgctggca tcgggagacg ggaccggcct gtgttgaagc ctgcccgaca
     8461 aaggcgttgc agtgcatgga tgtagagaaa gtgcagcggc atcggctacg gcagcagcct
     8521 gtttgaaacg tttttccggc gtttctcaac gccggaatag tgcgctttgt ttatgccaga
     8581 tgcggcgtaa acgccttatc cggcccacaa attcttgcaa attaaatata ttgcagaaat
     8641 catgtaggcc tgataagcgt agcgcatcag gcaattttta gtgactttca gcccaggctc
     8701 tttctatctc ttccgccaga atcttcaccc ccgcctcaat tttctccggc tctggtacgt
     8761 agttcatgcg catacattga tgcgtatgcg gccacggttt gtccagccct gggaagaagt
     8821 tgtgccccgg caccatcagc acgccgcgtg ctttcaggcg ctgatagagc tgctcggtcg
     8881 taatgggcaa atccttaaac caaagccaga ggaaaatggc tccttctggt ttatgaatta
     8941 ggcagcgatc ttccggtaaa tagcggcgaa tgatggcgat agtttcctga acacgctggt
     9001 agtaaaacgg tttgatgact gtttcagaca ggcgcagcag atcgttacgc ttaatcattt
     9061 cacacatcat cgccggacca ataccgccag gtgccaggct gataatgccg ttcatattgg
     9121 tgatggcggt gatgattttt tcattggcga tgataatgcc gcagcgggag ccaggcagcc
     9181 ccagcttgga aagactcatg cacagcacga tattcggatt ccatagcggg cgcgcttcac
     9241 tgaagatgat acccgggaac gggacgccat aagcgttatc aatcaccagc ggaatgccgt
     9301 gttgattcgc cagcgcgtca agcttcagca actcttcgtc agtaatcaca ttgcctgttg
     9361 gattcgtcgg ccgggagacg caaatcatcc cggtttcttc gccaatatgc agatgctcaa
     9421 aatcgacgtg gtatttaaac tggccttccg gcagcagttc aatattcgga cgcgcagaga
     9481 caaacagatc ttcttccagt ccggcgtcag cgtagccaat gtattccggt gccagcggga
     9541 acagcacttt tttgacccga ccatcggcac ggcgtccggc aaacagatta aataagtaga
     9601 aaaacgcgct ctggctgccg tttgttagtg caatattctg tgcttcgata tcccaaccca
     9661 acttctcgcg cagcattccg gcaagcagtg tgagtagctc cgttttcccc tgtggaccgt
     9721 cgtaattgca cagtgcatca gtcgctttgc cactttccag catgtcggtc agtagcgtct
     9781 ggaagtagtc ctgcatttcc gggatctgcg ccggattacc gccgccgagc ataatcgcgc
     9841 caggcgtgcg taaaccgtcg ttcagatctt ccatcagcag cgtaatgccg gagtggcggg
     9901 taaatttgtc accaaaaagg gagaatgtca tagcgggata tctgtcgaac tctgtagcaa
     9961 ggaaggtaac aataacgcta catttgctgg ggtgcaaatc ggtctgttga agagtgagcg
    10021 gtgctttatc ctgcaacgct gattagggct gacattttat cctggttggc ggcttcccga
    10081 ggtactgacg aacgggaagc cggaaaagtc actgttgccc agcccagatg accagcactt
    10141 tatcgtcgcc atgctcacga acaaagccgt agccctgctt cagcgaaagt gtcgtttgtt
    10201 tgcccgcgcc aattgcggga tggcgggcgc ggaactggct gattttctgc cagtgcgcga
    10261 cgttggcggc agatttaccg ctaacatcct gccagttcat atccgaacgt gtaccttgca
    10321 gcggatcaga acctgtagga ccgaacggac gcgaggattc atcgccataa aagatttgta
    10381 ccgcgcctgg cgctaatagt aataactctg ctgctttgtc gcccccttca cggaacagac
    10441 gggtatcatg cgacgagagg tagctcaaca cgttgaaacc ctgcaatttc tccgccattt
    10501 gctgccaggt cgtatccatc tgcgcaatac aatcgacagc tttcgccgcc tgctcctgat
    10561 aatcgaaatt gatcatcgca tcgaagccgt ggcgatagta gtcactttgc atcacgccgt
    10621 ggccccaggc ttcaccggtc atccagaaag gtttgtcatc taatgctttg tcggggttag
    10681 cttttttcca ttcgcgaagc gcggcgctgg cttcggtttt cagttgctgc caagcgggca
    10741 actcaacatg tttggcggta tcgacccgaa aaccatcaat cccatagtcg cggacccact
    10801 gacttaacca gtgggttaag taatcgcgag gggtaaagcc gtcgatggct ttagcgtggg
    10861 tatccgtttt gtttttatag aacaccggca gaccagaagc ggtagttgat tcggttttga
    10921 tatccggcaa aaaggctagc gacatggtga gatcgtcgaa tccaggattg tcgtaatcgc
    10981 cgatatcggt acggatccag ttttttcccc accatttatc ccagcctgtt ttgtcgctga
    11041 aattaatgta atcgttaaag ctatgccagg tttgcccggc ggcaggtttc cagtcgctcc
    11101 agcgttcacc cagcgttttt ttcacttcgt caccagaaag atataacgcg ccaaactgat
    11161 actcctgcat atccgccagc gtggcatagc cggtgtggtt catcacgaca tcaaagagaa
    11221 tacgaatacc gcgctgatgt gcgctatcaa ccagcgtccg tagatcggct tcgttgccca
    11281 tattggcatc aagattcgtc cagtcctgtg tgtaataacc gtggtaggca taatgcggga
    11341 aatcgccttt tgtaccgccg ccgacccagc cgtgaatttg ctcaaatggg gcgcttatcc
    11401 ataaagcatt aacgcccaac tgctggaggt aatccagttt gttggtcagg ccgcgtaaat
    11461 cgccgccgtg aaaagtgcca atttccgcca taccgtcttt atgacgtccg taactctggt
    11521 cattactggg atcgccgttt tcgaaacgat ctgtcagcac aaagtaaacc gtggcgttat
    11581 gccagtcgaa aggggcagag gcgtcagttt ctgcccgttc cagcaggagt aaaccgttgc
    11641 tggtagcagc gggttgtaac gttatttgac cgttcttcac tatcgcaatt tgctggctgt
    11701 aataatcccg tacggcggct ccttccggga aagtggcgct gacatccagc gtgagcggta
    11761 atccatccca tttcgggcat tcacggacca ggtttgctac cggttcggcg gcgttctgga
    11821 tggaaatcat caatgttggc gtaccggagc gggtgtctat ttgcagcgta tatttgccgt
    11881 ccctgaacaa tcgccattga ggcggcgtgt tgctacaagg ttgcagggaa agcatctgat
    11941 tgagttttat cgcatctgca ggctgccagc actgctggtc aaaatttagc gtgagtggac
    12001 gcgtaccttt gggcaactgc gcgtggctga caaatgttcc cgttccctgt tcgctaaagg
    12061 cagggaaccc cggagaagtc cagctggcgg caacggcgaa gccaggaagg agtgtcagaa
    12121 aacaggcggc gagtttcatg gtggatgagt ccttattgct gcgatttcag acagtttgcc
    12181 agcgataagc cagacaaaac tcatccctga gcggtaagtc cacaggatga gaagcaaggg
    12241 tgagcgatcg cgcgcaaaaa acggctgaat tttgcgataa accccacatt ttctgcgatt
    12301 tagcgccaat ctgaatcgtt aactcgtgac atagtttcag atttggacta ttccttgggg
    12361 tgctcttgac agctattttt gatatgattt gagattcaac tctcaaattt gtgaaaaata
    12421 taaggtgttg gaatgattaa atccgaccag gagacctgat gattttgact cccatacgac
    12481 gatatggggc gatgattctt atgttactca ctctggtgtt ttcgagtgag gtgttagcga
    12541 agacgcacac aacaacagcg agtcaaaagt cccacttaac taaagctagt aataaacagg
    12601 taagcagtaa acaagagtat tctcgcaata gtgcaaagag tagttcactt cctgatttgc
    12661 gaaaataccc ttccggaaca tcaaggaaaa aggcgtttct ccggaccgta atgccttaca
    12721 ttaccagcca aaatgcagcc attactgcag aacgtaactg gctgatttca aaacagtatc
    12781 agggccaatg gtcacctgct gagcgtgcgc gtctgaaaga catcgccaaa cgctacaagg
    12841 tgaagtggtc tggtaatacg cgaaaaatcc cgtggaatac cttgcttgaa cgcgtagaca
    12901 ttatccctac cagtatggtg gcgacgatgg ctgcagcaga aagcggttgg ggaacgtcga
    12961 agctggcgcg caacaacaac aatcttttcg gcatgaaatg catgaaagga cgttgtacca
    13021 atgcgccagg taaagtgaaa gggtactcac agtttagttc tgtcaaagaa tcggtgagcg
    13081 cctatgtcac taacctcaat acgcatccgg cttactcttc gttccgtaaa tcgcgtgcgc
    13141 agctgcgtaa agctgatcag gaagtgactg ccacagcgat gattcacaag ctgaagggct
    13201 actcgaccaa agggaagagt tataacaact acctgttcgc aatgtaccag gataaccaac
    13261 ggttaatcgc ggcgcatatg taattgtatt tcctcgagcc ttatccgact tgtcggtcgg
    13321 ataaggcttt tgatcgcatc aggtggttgt gctgagttac ctgatgcgac gccgacaatt
    13381 ctcatcatcg ctacaacatg acctcgctat ttacatcgcg atactctttt ggcgtcgtgt
    13441 catatgcttt tttaaaaaca gagtagaaat attgcagcga tggataaccg cacatttgcg
    13501 atatctcatt gatcgacaag gtggttgaaa tcagcagact gcgcgctttc tccagcttct
    13561 cagcatgaat catggcatgg atggtttcac ccacctcttc tttaaaacgc ttctcaagat
    13621 tggagcgcga gatcccgacc gcatcgagta cctgatccac tttaatccct ttacaggcgt
    13681 gattacgaat gtaatgcatg gcctgaataa cggcgggatc ggtcagcgaa cggtaatctg
    13741 ttgagcgccg ttcaatgacg cgaactggag ggaccaaaat ccgctgtagc ggcatttctt
    13801 ctttatctaa taatcgatgc aacagttttg ccgcctgata gcccatttgc cgcgcgccct
    13861 gagcgaccga agaaagggcg acacgcgaca gatagcgggt cagttcttcg ttatcgatgc
    13921 caatcacgca taatttttcc ggtacgggaa tgtgtagatg ttcacatact tgcagaatat
    13981 gccgcgcccg ggcgtcagta acggcaataa tcccggtttg cggtggcagt gtttgtagcc
    14041 agtctgccag ccgattttgc gcgtgttgcc agttctctgg tgcggtttct aacccctgat
    14101 aaaccactcc gcgatacttt tcttcggcga caagctgacg aaatgcatat tcgcgttcag
    14161 tggcccaacg tttgccgctt gattccggaa gaccataaaa agcaaagcgg ttaacgcctt
    14221 tctcttttaa atgcaaaaat gcgctttcaa ccagcgcata gttatcggtg gcaatgtaat
    14281 gaacgggtgg gtaactttct gcaaggtgat acgagccgcc aaccccaaca atggggacgt
    14341 cgacatcagc cagcgcttgc tcgatctgtt tgtcgtcgaa gtcggcaatg acgccatctc
    14401 ctaaccagtc cttgatttta tcaatgcggg cgcggaaatc ttcttcaatg aaaatatccc
    14461 attccgattg tgacgcctgt aaatattccc ctacgccttc tactacctgc cggtcatagg
    14521 ctttattggc attgaacagt aatgtgatgc ggtgacgttt agtaaacatg gttcttttcc
    14581 tgctgaatca tgcaaaaact caaaaccggt aatacgttac cggctttgag aaaattttta
    14641 tcaaaatcaa gaacggcgtt tggttgcgga gtccatccat actgccagca acagaatcgc
    14701 acctttaacg atatactgcc agaaggtcgg tacatccatc atactcatac cgttatccag
    14761 tgaagccatg atgaatgccc ccattaccgc tccagccacg ctcccgacgc cgcctgccag
    14821 actggtgccg ccaatcacgc atgctgcaat tgcgtccagc tcggcgatat tccccgcaga
    14881 aggtgaacca gcgccaagtc gagaactgag gattaatccg gcgatggcta ccattaatcc
    14941 gttaatagcg aacacggcaa gtttggtgcg ttcaacgtta attccggaga gacgtgctgc
    15001 ttccagattg ccgccgatgg cataaatgcg tcgtccaaat gctgtccgcg ttgccataaa
    15061 cattccgccg agtaacagca acgtcagcag cagaacagga gtaggaacgc cacggtaatc
    15121 attcaacagc cagattgcgc ctaatacgat gatagcggtt aaagcctggc gaccgactac
    15181 tgcggtagag gctggagact gcaaacccaa agcctgacgg cgcattcttc cgcgccattg
    15241 ccagccaaca aaagccatta agccaagcgc gccaatgatg aagccggtgc tggcggggag
    15301 atagctttgc ccaatttgtg acatcgcggc gctggtgggg gatacagtcg tgccgttggt
    15361 gatgccaatg agtatgccgc gaaatgccaa catgcccgcg agagtgacaa taaatgaagg
    15421 gactttgcgg tacgcgaccc accatccgtt ccaggcaccg agaagcagtc ccagaaccaa
    15481 cgtcacaatg atggtaagtg gcaaaggcca gcctagccag acgtcacaaa tcgccgcgac
    15541 gccacctaat agccccatca ttgagccgac ggaaaggtcg atttcagcag aaattatgac
    15601 gaacaccatt cctaccgcga ggatgccggt aatcgcggtc tggcgtaaca ggttggagac
    15661 gttacgggcg cttaagtagg caccatcggt ggtccaggta aagaacagca tgattgcgat
    15721 gatagctgca atcatcacga agacctgcaa attcagtgat ttcagcccgg agaagccacc
    15781 ggatgtcggt acggccaatt tcacttcaga cggattgctt ttcgacatga tgttcgctcc
    15841 tcaatgcggc ttccatcacc tgctcctgag tcaggttatg atttatcagg ttggctttta
    15901 gtttcccttc atgcattact agtacacgat cgctaaggcc gagcacttca ggtaattcag
    15961 aagagatgac aataacggca ataccctgct ggacgagttg gttaattaat ttgtagatct
    16021 cgtatttcgc gccaatatcg atacccctgg tgggttcatc aagaatgaga atgcgcgggt
    16081 taagtaacag acagcgagcg aggatcgctt tttgctgatt gccgccgctc aaacgtccaa
    16141 tagcaaggtc gggggacgac gttttaactt tgagttgctg gattgattcc agaatacatt
    16201 tttgctctgc cgcgtcatca agctggctaa tgccaccggt aaatttattg agtgcggcga
    16261 gggtaatatt tttaccaacc gccattaccg gaacgatgcc gtcgcgcttt ctgtcttcgg
    16321 ggaccatcgc aatcccctgg gcgatggctt gctgacagtt acgaatatct acctgtttgc
    16381 catcaatata aatttttcct tcccattgtc cgggccacac accaaacagg cactgaatgg
    16441 tctcggtacg tccggcacca acgagtccgg caatacccag tatttcgcca cgtttcaggg
    16501 aaaacgagac atcattaact cgtttaatat gacgattaac cggatgccat gccgtcagat
    16561 gttcaatacg taatatttca tctccggtgg tatgtggttc attagggtaa agcgcggtta
    16621 actctcgccc gaccatcatg gtgataatat cgtcttcact cattccggca gcatcacgcg
    16681 taccaatgtg ctgtccgtcg cgaataacgc aaatcgtatc ggaaatcgct ttgacttcgt
    16741 tgagtttgtg cgaaatataa atacaggcga taccgtgctg ttgtagatcg cgaataatat
    16801 ccagtaaaac cgacgtttcc tgctcagtta atgaggctgt cggttcatcg agaattaaca
    16861 agcgcacctg tttattaagt gccttggcaa tttcaaccag ttgttgttgc ccaagcccta
    16921 aatcgccaac gcgggtatca ggtgaaatgg ataaactgac ctgtgcgagc agcttctgac
    16981 agcgtagcgt catcaggtca taatccataa tgccattgtg ggttatttcg ttacccagga
    17041 agatattttc cagcacggtc aattctttca ccagggccaa ttcctggtga atgatggcga
    17101 tacctttgcg ttcggtatcg cggatgtgac tcgcctgaat ctcttctccc gcaaaaataa
    17161 tttcgccttc gtaggagcca tggggataaa taccacacag cactttcatc agcgttgatt
    17221 taccagaccc attttcccca cagagtgaga cgatttcgcc agcattcaac cgcaagctga
    17281 cgttatcaat cgccttcaca ctgccgaagg ttttggtaat gttcttcatt tcaagtagat
    17341 aaggcatagc gactccacct aagccaattc attcacgcgg catggagaga aatcacgccc
    17401 ccgctccgcg ctggggcgta acgcttacag ctcgctctct ttgtggaatc cgtctttaat
    17461 taccgtatct ttgatgttgt ttttattcac atcgatcggt gtcaggaggc gggaggggac
    17521 atctttcagg ccattattta gtgaagtatc tgcttttggc tcctgaccat tgcccaattc
    17581 aacggcaatt tctgcggcag tatttgccag caatgtaata ggtttatata ccgtcatagt
    17641 ttgcgtaccg gcagcaatac gtttaatacc tgcgagatcc gcatcctggc cagaaattgc
    17701 tactttccct gataaacctt gcgcgcttaa tgcctgaatt gccccacctg cggtggcatc
    17761 gtttgaggca actacagcat caattttgtt attattggcg gttagcgcgt tttccataat
    17821 tttcaatgcg ttttccggta accagccatc aacccattgg tcaccaacga ctttaatttt
    17881 tccggaatca acgtaaggtt gtaacacttt catttgtccg gcgcggaaca gcttagcgtt
    17941 gttatctacc ggcgagccgc ccatcaggaa gtaattacct tgcggaacaa tatcgaccag
    18001 ggcttttgcc tgcagttcac cgactttttc gttatcgaaa gaaatataaa aatcgatatc
    18061 cgcatcgtta atcatacggt cgtaagctaa tactttaatg ccttcttgtt tggcttcttt
    18121 tacaacgtta cttaatacct gaccgttata cggaataatg acaagaacat cgacaccccg
    18181 gtttatcatg ttttcaatct gcgacatttg tgtttcttca ttgccatttg cagactgtac
    18241 aaatactttc gcgccgagag attctgcctt tttcacaaag atatctcgat ctttttgcca
    18301 gcgttcaaga cggagatcat caatcgccat acctattttg acttctttgg cgtgtgcagc
    18361 aacgttggta agcaggagtg aggtgcaaag ggtgagtaga atgttcttta ttttcatggt
    18421 gtagggcctt ctgtagttag aggacagctt taataagtaa caatcaccgc gataaacgta
    18481 accaattttt agcaactaaa caggggaaaa caattacaga tttttatctt tcgattacga
    18541 tttttggttt atttcttgat ttatgaccga gatcttactt ttgttgcgca attgtactta
    18601 ttgcattttt ctcttcgagg aattacccag tttcatcatt ccattttatt ttgcgagcga
    18661 gcgcacactt gtgaattatc tcaatagcag tgtgaaataa cataattgag caactgaaag
    18721 ggagtgccca atattacgac atcatccatc acccgcggca ttacctgatt atggagttca
    18781 atatgcaagc ctattttgac cagctcgatc gcgttcgtta tgaaggctca aaatcctcaa
    18841 acccgttagc attccgtcac tacaatcccg acgaactggt gttgggcaag cgtatggaag
    18901 agcacttgcg atttgccgcc tgctactggc acaccttctg ctggaacggg gcggatatgt
    18961 ttggtgtggg ggcgtttaat cgtccgtggc agcagcctgg tgaggcactg gcgttggcga
    19021 agcgtaaagc agatgtcgca tttgagtttt tccacaagtt acatgtgcca ttttattgct
    19081 tccacgatgt ggatgtttct cctgagggcg cgtcgttaaa agagtacatc aataattttg
    19141 cgcaaatggt tgatgttctg gcaggcaagc aagaagagag cggcgtgaag ctgctgtggg
    19201 gaaccgctaa ctgctttaca aaccctcgct atggcgcggg tgcggcgacg aacccagatc
    19261 cagaagtctt cagctgggcg gcaacgcaag ttgttacagc gatggaagca acccataaat
    19321 tgggtggtga aaactatgtc ctgtggggcg gtcgtgaagg ttacgaaacg ctgttaaata
    19381 ccgatttgcg tcaggagcgt gaacaactgg gccgctttat gcaaatggtg gttgagcata
    19441 aacataaaat cggcttccag ggcacgttgc ttatcgaacc gaaaccgcaa gaaccgacta
    19501 aacatcaata tgattacgat gctgcgacag tctatggctt cctgaaacag tttggtctgg
    19561 aaaaagagat taaactgaac attgaagcta accacgcgac gctggcaggt cactctttcc
    19621 atcatgaaat agccaccgcc attgcgcttg gcctgttcgg ttctgtcgac gccaaccgtg
    19681 gcgatgcgca actgggctgg gacaccgacc agttcccgaa cagtgtggaa gagaatgcgc
    19741 tggtgatgta tgaaattctc aaagcaggcg gtttcaccac cggtggtctg aacttcgatg
    19801 ccaaagtacg tcgtcaaagt actgataaat atgatctgtt ttacggtcat atcggcgcga
    19861 tggatacgat ggcactggcg ctgaaaattg cagcgtgcat gattgaagat ggcgagctgg
    19921 ataaacgcat cgcgcagcgt tattccggct ggaatagcga attgggccag caaatcctga
    19981 aaggccaaat gtcactggca gatttagcca aatatgctca ggaacataat ttgtctccgg
    20041 tgcatcagag tggtcgccag gagcaactgg aaaatctggt aaatcattat ctgttcgaca
    20101 aataacggct aactgtgcag tccgttggcc cggttatcgg tagcgatacc gggcattttt
    20161 ttaaggaacg atcgatatgt atatcgggat agatcttggc acctcgggcg taaaagttat
    20221 tttgctcaac gagcagggtg aggtggttgc ttcgcaaacg gaaaagctga ccgtttcgcg
    20281 cccgcatcca ctctggtcgg aacaagaccc ggaacagtgg tggcaggcaa ctgatcgcgc
    20341 aatgaaagct ctgggcgatc agcattctct gcaggacgtt aaagcattgg gtattgccgg
    20401 ccagatgcac ggagcaacct tactggatgc tcaacaacgg gtattgcgcc ctgccatttt
    20461 gtggaacgac gggcgctgtg cgcaagagtg cgctttgctg gaagcgcgag ttccgctatc
    20521 gcgggtgatt accggcaacc tgatgatgcc cggatttact gcgcctaaat tgctatgggt
    20581 tcagcggcat gagccggaga tattccgtca aatcgacaaa gtattattac cgaaagatta
    20641 cttgcgtctg cgtatgacgg gggagtttgc cagcgatatg tctgacgcag ctggcaccat
    20701 gtggctggat gtcgcaaagc gtgactggag tgacgtcatg ctgcaggctt gcgacttatc
    20761 tcgtgaccag atgcccgcat tatacgaagg cagcgaaatt actggtgctt tgttacctga
    20821 agttgcgaaa gcgtggggta tggcgacggt gccagttgtc gcaggcggtg gcgacaatgc
    20881 agctggtgca gttggtgtgg gaatggttga tgctaatcag gcaatgttat cgctggggac
    20941 gtcgggggtc tattttgctg tcagcgaagg gttcttaagc aagccagaaa gcgccgtaca
    21001 cagcttttgc catgcgctac cgcagcgttg gcatttaatg tctgtaatgc tgagtgcagc
    21061 gtcgtgtctg gattgggccg cgaaattaac cggcctgagc aatgtcccag ctttaatcgc
    21121 tgcagctcaa caggctgatg aaagtgccga gccagtttgg tttctgcctt atctttccgg
    21181 cgagcgtaca ccacacaata atccccaggc gaaaggggtt ttctttggtt tgactcatca
    21241 acatggcccc aatgaactgg cgcgagcagt gctggaaggc gtgggttatg cgctggcaga
    21301 tggcatggat gtcgtgcatg cctgcggcat taaaccgcaa agtgttacgt tgattggtgg
    21361 cggggcgcgt agtgagtact ggcgtcagat gctggcggat atcagcggtc agcagctcga
    21421 ttaccgtacg gggggggatg tggggccagc actgggcgca gcaaggctgg cgcagattgc
    21481 ggcgaatcca gagaaatcgc tcattgagtt gttgccgcaa ctaccgttag aacagtcgca
    21541 tctaccagat gcgcagcgtt atgccgctta tcagccacga cgtgaaacat tccgtcgcct
    21601 ctatcagcaa cttctgccat taatggcgta aacgttatcc cctgcctgac cgggtggggg
    21661 ataattcaca tctatatatc tcagcaatta attaatattt agtatgaatt tattctgaaa
    21721 atcatttgtt aatggcattt ttcagttttg tctttcgttg gttactcgta atgtatcgct
    21781 ggtagatatg gagatcgtta tgaaaacctc aaaaactgtg gcaaaactat tatttgttgt
    21841 cggggcgctg gtttatctgg ttgggctatg gatctcatgc ccattgttga gtggaaaagg
    21901 ctattttctt ggcgtgttaa tgacagcaac ttttggcaac tatgcatatc ttcgcgcaga
    21961 aaaactcggg caactggata atttttttac ccatatttgc cagttagttg cgttaatcac
    22021 tatcggtctc ttgtttatcg gtgttttaaa tgcacctatc aatgcttatg aaatggtgat
    22081 ctaccccatc gccttttttg tctgcttgtt tggtcaaatg cgtttgtttc gctcggtatg
    22141 agcaacataa agctcttaca cattcaggaa tgaaaggaat actgtgatgg acaacaaaat
    22201 atcaacctat tcaccggcct ttagtattgt gtcatggata gctctcgttg gtggtatcgt
    22261 tacctatctg ttagggctat ggaatgcaga gatgcagtta aatgaaaaag gatattattt
    22321 tgccgtactg gtattaggac tgttttctgc ggcgtcttat caaaagaccg ttcgggacaa
    22381 gtatgaaggc ataccgacca cttccattta ttatatgacc tgcctgactg tctttattat
    22441 ctctgttgca ttactgatgg taggtctgtg gaatgcgaca ttattactca gcgaaaaagg
    22501 tttttatgga ctggctttct tcttaagctt gtttggtgca gtagcggtgc agaaaaatat
    22561 tcgtgatgcc ggaataaacc caccaaaaga aacacaggtt acccaggaag aatacagcga
    22621 ataactcacg taagcccggt cagtccaatg tgaccgggct tttacttaac tcactaatct
    22681 gtttctgtcg attcgttgta ccagcataga aagtaacaaa ctcgctgcca acgtcgcgca
    22741 aaaaatccaa ataatatcca gtattggcca atttttaagc tcaattcccc gggtgcgcag
    22801 cgcatggata atcaaggcgt ggaatccgta tatacccaac gaatggcggg agattaagcc
    22861 aagtccgcga atggtacgcg tatccagcgt gtttttaacc agagtcaata gcgcgattgc
    22921 gcagataaaa accatcggcc cacagtaagg ataccaggta tcggcaaaat ttccgcgcca
    22981 ctgcaattca tataatgtcc cgcgagagat aataaaaact cccgtcgcaa acagtgcggc
    23041 gctcacccac gacagtgctt tatgctgtgt gtccatcatc cctaaagcgc ggcccaacat
    23101 gccatacaga atgtagtaaa aagtatcgcc attgatatat aagttaattg gcagccattc
    23161 aaaaccgtcg attttctgcg gcacggtgtt tgggttagcg atgataccaa tcaccaccat
    23221 tagcaccagc aacatttttc cgccgacgtt cttcacctga atcagcggtg aaaccagata
    23281 aatcaccgca atcgcgaaga aaaaccataa gtggtaaaac actggctttt gcagcaggtt
    23341 tttcagcgct aactccacat taatggaggt aaacagtgca atgtagagca gcgcgattgc
    23401 gctataaaaa aacagacata agccgatacg caagaaatgg cgcggctggg cgctgcgttc
    23461 gccaaaaaag agatagccgg aaatcatgaa aaatagcggc acgctgacac gagaggcaga
    23521 gttcagaaca ttggcgatat cccaggtgac ggggctaaca ctatgagcat tggtcacata
    23581 ccaggtagtg gtgtgaatca tcaccaccat taaacacgct atccctcgca ggttatcaat
    23641 ccagtaaatt ttgggctgca tctgtgtctc tgtatctggt taaaaaaagt ctgaccgata
    23701 aatcattcag ttggcgcact ggaataatct gagttttaac actacagctt atagaggctt
    23761 aaggaaattc gtaagatatc agccactata ccgatataaa taataagact cacctgcaaa
    23821 ccagacggta atttaatgat gatgaactct ttctttccgg caatggcgct tatggtgcta
    23881 gtgggttgtt ctacaccgtc acccgtgcag aaagcacaac gggtaaaggt tgatcctctg
    23941 cgttcgttga atatggaagc gttatgcaag gatcaggcgg caaaacgtta taacaccggc
    24001 gagcaaaaaa tcgacgtcac cgccttcgaa cagttccagg gaagctatga aatgcgcggt
    24061 tataccttcc gtaaagagca gtttgtctgt tcttttgacg cagatggcca ttttttgcat
    24121 ctttccatgc gttaagccct gctttttccc gtttcatact gtatatcttc catccagcgg
    24181 gtatactgat cccttccttt aaatccacac gtatccagca cgaaataata tgcaaaagtt
    24241 tgataccagg accttccagg gcttgatcct gaccttacag gattactggg ctcgccaggg
    24301 ctgcaccatt gttcaaccat tggacatgga agtcggcgcg ggaacctctc acccaatgac
    24361 ctgtctgcgc gcgctggggc cagaaccgat ggcggctgct tatgttcagc cttctcgtcg
    24421 cccgaccgat ggtcgctacg gcgaaaaccc caaccgttta cagcactact atcagttcca
    24481 ggtggtcatt aagccatcgc cggacaatat tcaggagctg taccttggtt ctctgaaaga
    24541 gctgggcatg gacccgacta tccacgatat ccgtttcgtg gaagataact gggaaaaccc
    24601 gacgctgggt gcctggggac tgggctggga agtgtggcta aacggcatgg aagtgacgca
    24661 gttcacttac ttccagcagg ttggtggtct ggagtgtaaa ccggttaccg gcgagatcac
    24721 ctacggtctg gaacgtctgg ccatgtacat tcagggcgta gacagcgttt acgacctggt
    24781 ctggagcgac ggcccgctgg gtaaaaccac ctacggcgac gtgttccatc agaacgaagt
    24841 ggagcagtcc acttacaact tcgaatacgc ggatgtggac ttcctgttca cctgctttga
    24901 gcagtacgag aaagaagcgc agcagctgct ggcgctggaa aatccgctgc cgctgccagc
    24961 ttacgagcgt attctgaaag ccgcccacag cttcaacctg ctggatgcgc gtaaagccat
    25021 ctccgtcacc gagcgtcagc gctacattct gcgcattcgc accctgacca aagcagtggc
    25081 agaagcatat tacgcttccc gtgaagccct aggcttcccg atgtgcaaca aagataagta
    25141 agaggcggct atgtctgaga aaacttttct ggtggaaatc ggcactgaag agctgccacc
    25201 aaaagcactg cgcagcctgg cagagtcctt tgctgcgaac tttactgcgg agctggataa
    25261 cgcaggcctc gcacacggca ccgttcaatg gtttgctgct ccgcgtcgtc tggcgctgaa
    25321 agtagctaac ctggcggaag cgcaaccgga tcgtgaaatc gaaaaacgcg gcccggcgat
    25381 tgcccaggcg ttcgacgctg aaggcaaacc gagcaaagcg gcagaaggtt gggcgcgtgg
    25441 ttgcggtatt accgttgacc aggctgagcg tctgactacc gataaaggcg aatggctgct
    25501 gtatcgcgcc catgtgaagg gcgaaagcac tgaagcactg ctgccgaata tggttgcgac
    25561 ttcgctggcg aaactgccga ttccgaaact gatgcgctgg ggtgcaagcg acgtgcactt
    25621 cgtgcgtccg gtgcacaccg tgaccctgct gctgggcgac aaagtcattc ctgcaaccat
    25681 tctgggcatt cagtccgatc gcgtgattcg cggccaccgc tttatgggcg agccggaatt
    25741 caccatcgat aacgccgatc agtatccgga aattctgcgt gagcgtggga aagtcatcgc
    25801 cgattacgaa gaacgtaagg cgaagattaa agccgatgcc gaagaagcag cgcgtaagat
    25861 tggcggtaac gctgacttaa gcgaaagcct gctggaagaa gtggcttcgc tggtggagtg
    25921 gccggtcgtt ctaaccgcga aatttgaaga gaaattcctc gcggtgccgt ctgaagcact
    25981 ggtttacacc atgaaaggtg accagaagta cttcccggtg tatgcgaacg acggcaaact
    26041 gctgccgaac tttatcttcg ttgccaacat cgaatcgaaa gatccgcagc aaattatctc
    26101 tggtaacgag aaagtcgttc gtccacgtct ggctgatgcc gagttcttct tcaacaccga
    26161 ccgtaaaaaa cgtctggaag ataacctgcc gcgcctgcaa accgtgctgt tccagcaaca
    26221 gctgggtacg ctgcgtgaca agactgaccg catccaggcg ctggctggct ggatcgccga
    26281 acagattggc gctgacgtta accacgcaac ccgtgcgggc ctgctgtcta agtgtgacct
    26341 gatgaccaac atggtcttcg agttcaccga cacccaaggc gttatgggga tgcactacgc
    26401 gcgtcacgat ggcgaagcgg aagacgtagc cgtcgcgctg aatgagcagt atcagccgcg
    26461 ctttgccggt gatgacctgc cgtccaaccc ggtagcttgt gcgctggcga ttgctgataa
    26521 gatggatact ctggcgggta tcttcggtat cggtcagcat ccgaaaggcg acaaagaccc
    26581 gtttgcgctg cgtcgtgccg cacttggcgt gctgcgaatt atcgttgaga agaacctcaa
    26641 ccttgatctg caaacgctga ccgaagaagc ggtgcgtctg tatggcgata agctgactaa
    26701 tgccaacgta gttgatgatg ttatcgactt tatgctcggt cgcttccgcg cctggtatca
    26761 ggacgaaggt tataccgttg acaccatcca ggcggtactg gcgcgtcgtc cgactcgtcc
    26821 ggctgatttc gatgcccgca tgaaagcggt atcgcacttc cgtaccctgg aagcagctgc
    26881 agcactggcg gcggcgaaca aacgtgtatc taacatcctg gcgaaatctg acgaagtgct
    26941 gagcgatcgc gtgaatgcct ctactctgaa agagccggaa gaaattaaac tggcgatgca
    27001 ggttgtggtg ctacgtgaca agctggagcc gtactttgcg gaaggtcgtt accaggatgc
    27061 gctggtcgaa ctggcagagc tgcgtgaacc agtcgatgcc ttcttcgata aagtgatggt
    27121 catggttgat gacaaagaat tgcgtatcaa ccgtctgacc atgctggaga aactgcgcga
    27181 actgttcctg cgcgttgcgg atatttcgct gttgcagtaa taacgccgtt attaaaaagc
    27241 ctgccatctg gcaggctttt tttatcgcta aataatacag caacctttaa taatcttctg
    27301 ctgaataaag attatctcat atattaattt tatgagattt ttttaggatt atatcaagga
    27361 gaagaaacaa acttattaag ctagaatagc cacgggtgct tgagactgtt tgtctcaggt
    27421 attcaccgaa aggcagacag agaaaagccc cacctgacta taaatcaagg tgaggcccct
    27481 ctatgtatag acaccataag gttagcctct tactcgtt
//
LOCUS       DBFIFI010000047        27008 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.047, whole genome shotgun sequence.
ACCESSION   DBFIFI010000047 DBFIFI010000000
VERSION     DBFIFI010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27008
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.047"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..1270
                     /locus_tag="N7B39_001881"
     CDS             <1..1270
                     /locus_tag="N7B39_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HGU2421875.1"
                     /translation="APLPQWANAPAVTPVAQLSLQESILRAFARNPGVTQQAAQIGIG
                     EAQIDEAKSAWYPHVGLTGNAGPSRQTDSSGRLDNNVSYGITLTQLVYDFGKTNNDIN
                     LQTAARDSYRFKLMATLTDVAEKTATAYMEVSRYQALCDAAQRNIHSLENVYNMAALR
                     ANAGLNSSSDELQAQTRIAGMRSTLEQYQAQMASAKAQLAVLTGVQPEAIAAPPAELA
                     EQPVSLKNIDYQSIPLVLAAENLRQSAQYGVEKTKAQYWPTLSIQGGKTRYQTSDRSY
                     WDDQLQLNVNAPLYQGGAVSAQVQQAEGQQKISASQVEQAKLDVLQRASVAYANWTGA
                     RGREEAGLAQSESAHKTRDVYQNEYKLGKRSLNDLLTVEQDVFQAQSAEINANYDGWV
                     AAVNYAAAVNNLIPLAGIKQGLYNDLPDLK"
     gene            1373..5758
                     /locus_tag="N7B39_001882"
     CDS             1373..5758
                     /locus_tag="N7B39_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HGU2421876.1"
                     /translation="MSLIIDVISRKTSVKQTLINPGDVTVVIYEPSVVQVHAQASAVA
                     RYVREGNDLLIYMRDGTVIRCNGYFLQAANTAEQSELVFADGQQLTHITFADTAAGGL
                     APVELTAQTTAIESIAPFLDTVAQTSAFPWGWLAGAAVGGGALGALLASGGDGDSKTE
                     VINNPTPPAEPGNATPSFLVTDNQGDQRGILATNDITDDTTPTFSGSGQAGATIQIKD
                     SNGNTIASTQVDNNGHWSVSLPTQSAGEHTWSVVQIVGSTITDAGSITLTIDNSQASV
                     QVATTAGDNIINASEQAAGFTLSGTSSHLAQGTELTVTLNGKTYTTSVGANGAWSVQV
                     PTADAQALGEGNQAVLVSGKDATGNTVTGAQLLTVDTQPPTLAINTIAQDNIISAAEH
                     NVALVLSGTSNAEAGQTVTLTVNGKSHTATVGSDGTWQVTLPATEVQALAEGNYAVNA
                     SVSDRAGNTTSHSANFTVDTSAPVVSVNTVAGDDILNNAEQAVAQIISGQVSGASPGD
                     TVTVKLGTHVLTGIVLADGSWNVALDPAVTRTLDRGANTIFVTVTDAAGNTGAASRAI
                     TLVGVSPLITINTVSGDDIISGAEKGAPLTLTGSTQQAETGQTVTVTLAGQSFTTTVQ
                     ADGSWSLTVPAAAMGNLPDGAVAITASVTDLSGNTGNTSRTITVDSQAPALSIDPLTA
                     DNIINAAESGQDLPITGTTDAQPGQTVTVTLNGQTYQGVVQPDGTWSVTVPAANVGAL
                     ADGNATVTASVNDVAGNPSSVSRVALVDATPPVVTINPVATDNVINTPEHAQAQIISG
                     TVTGAQAGDIVTVTLNNVDYTTVVDGSGNWSLGVPASVVSGLADGSYPVSVSVTDKAG
                     NTGSQSLTVTVNTAAPLIGINSIAGDDVINASEKGADLQITGTSDQPVNTAITVTLNG
                     QNYTTTTDASGNWSVTVPASAVTALGQANYTVTAAVTSDIGNSATASHNVLVDSALPG
                     VTINPVATDDIINAAEAGVAQTISGQVTGAEDGDTVTITLGGNTYTATVGSNLTWSVD
                     VPAADIQALGNGDLTVNASVTNQNGNTGSGTRDITIDANLPGLRVDTVAGDDVVNIIE
                     HGQALVVTGSSSGLAESTPLTVTINNVEYTTAVQADGSWSVGVTAAQVSAWPAGTVNI
                     AVSGESSAGNSVSITHPVTVDLTPAAITINTIATDDVINAAEKGADLTLSGTTTNVEP
                     GQTVTVTFGGKNYTASVASDGSWTATVPAADLASLPEGSASALASVSNINGNSASAVH
                     NYSVDSSAPTIIINTVASDNIVNASEADAGVTVSGSTTAEAGQIVTITLNSPTVQTYQ
                     ATVQADGSWSINIPAADLEALTDGSHTLTATVNDKAGNPASTTHNLAVDLTVPVLTIN
                     TIAGDDIINATEHGQALVISGSSTGGEAGDVVTVTLNSKTYTTTLDASGNWSVGVPAA
                     DVTALGSGPQTVTATVTDAAGNSDN"
     gene            5810..21522
                     /locus_tag="N7B39_001883"
                     /pseudo
     CDS             5810..21522
                     /locus_tag="N7B39_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308569.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
     gene            21526..23688
                     /locus_tag="N7B39_001884"
     CDS             21526..23688
                     /locus_tag="N7B39_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HGU2421877.1"
                     /translation="MKKNAQSVEAWLEAMIAVARYYRLDFSQENVRATVNWERDSKRE
                     ELLTDMARQLGMGLRLVEFSADSLNPWRLPLIAVFDNQQIGVITRRDNHDNISVQFSG
                     DEGLETTLNVADIEDKIVELALLRPLSAIPDARVDDYIRPYQANWFWSLSLKDWRRYG
                     DIMLASLVANVLALAAMIFSMQVYDRVVPAQSYPTLWVLFAGVMMAILFEFCMRMVRT
                     HLSDVIGKRADLRISDRVFGHALRLKNNVRSKSTGSFISQIRELESVRELITSTTIGA
                     VADLPFFLLFVFILWMIGGWLVLVVLLALLLLVIPGLLVQRPLARLANEGMRESAVRN
                     ATLVEAVQSIEDIKLLRAEQRFQNQWNHTNDVASSISMKQRFLTGLLLTWTQEVQSIV
                     YVVVLLVGCFMVMNGDMTTGALVGTTILASRTIAPLSQISGVLSRWQQAKVARNGLDE
                     LMKRPVDQPEHGKLVHKAVLHGNYQFSNAVFYYDEEEKIADVAIGKLNIQAGEKIAIL
                     GRNGAGKSTLLQMLAGMRIAQQGQVLLDNISIGQLDPADLRRDMGLLSQTGRLFFGSL
                     RENLTMGMPEASDEDIERALTLSGALPFVQKQKNGLNYMIQEGGFGLSGGQRQTLLLA
                     RLLISQPNIVLLDEPSASLDEMAEAYLIEQLKQWIGHRTLIIATHRTAMLQLVDRIIV
                     MDQGRIVMDGAKEAILREQGEPTARRVVLQEKNKGSAA"
     gene            23685..24860
                     /locus_tag="N7B39_001885"
     CDS             23685..24860
                     /locus_tag="N7B39_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HGU2421878.1"
                     /translation="MNGQLQYNRGLTEPRLPRSALAVRVTAVMLLCFLGWAWYFQLDE
                     VTTGSGTVEPSGREQVVQSLEGGILYHLDVKVGDIVEQGQPLAQLNRTKTESDVQEAM
                     SRLYAALATSARLRAEVSNKPLVFPDELNKFPELIESETALYNTRRDGLNKATTGLTQ
                     GISLVNRELAMTQPLVKQGAASSVEVLRLQRQANELENKLSDVRTQYYVQAREELAKA
                     NAEVETQRSVIRGREDSLTRLNFTAPVRGIVQDIDVTTVGGVIAPGGKLMTIVPLDEQ
                     LLIEAKISPRDVAFIHPGQKSLVKITAYDYSIYGGLPGEVAVISPDTVQDEVRRDVYY
                     YRVYIRTFSNHLENKSKQQFPIFPGMVATVDIRTGKKSVLDYLLKPFNKAQEALRER"
     gene            24857..25264
                     /gene="cueR"
                     /locus_tag="N7B39_001886"
     CDS             24857..25264
                     /gene="cueR"
                     /locus_tag="N7B39_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HGU2421879.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     NQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(25268..25471)
                     /locus_tag="N7B39_001887"
     CDS             complement(25268..25471)
                     /locus_tag="N7B39_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2421880.1"
                     /translation="MKEQRKIPLTHIMIIGAFIFAFLQVVLLASLVHAVNVNNEIQEG
                     LFQSGRIMVESLQHILSVQTGIH"
     gene            complement(25468..25890)
                     /locus_tag="N7B39_001888"
     CDS             complement(25468..25890)
                     /locus_tag="N7B39_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGU2421881.1"
                     /translation="MMNSSIKSFSLLAVILLAGCSSPTSRIADCQAQGVSHDTCYLAE
                     QQRQAAILSASEAQAFKNAEAAQHAQAAKKAIYKGFGMTFRMSSKNFAYLNDSLCAID
                     EDNKDATVYQSGLYNVIVYHHTGKVALMKEGQFVGYLK"
     gene            complement(25975..26991)
                     /locus_tag="N7B39_001889"
     CDS             complement(25975..26991)
                     /locus_tag="N7B39_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HGU2421882.1"
                     /translation="MKTVNVALLALIISATSSPVVLAGDTIEAAATELSAINSGMSQS
                     EIEQKITRFLERTDNSPAAYTYLTEHHYIPSETPDTTQTPTVQTDPDAGQKTVAATGD
                     VQTTARYQSMINARQSAVTDAQQTQITEQQAQIVATQKTLAATGDTQNTAHYQEMINA
                     RLAAQNEANQRTATEQGQKMNALTTDVAVQQQNERTQYDKQMQSLAQESAQAHEQIDS
                     LSQDVTQTHQQLTNTQKRVADNSQQINTLNNHFSSLKNEVDDNRKEANAGTASAIAIA
                     SQPQVKTGDVMMVSAGAGTFNGESAVSVGTSFNAGTHTVLKAGISADTQSDFGAGVGV
                     GYSF"
BASE COUNT         6163 a         7863 c         7981 g         5001 t
ORIGIN      
        1 tgcaccgctg ccacaatggg cgaatgcccc agcggtaacg ccagttgccc aattatcttt
       61 acaggaaagt atattacgcg cctttgcgcg aaatcccggt gtcactcaac aggcggcgca
      121 gataggtatt ggcgaagcgc aaattgatga agccaaaagt gcctggtatc cgcacgttgg
      181 attaaccggc aacgcggggc cgtcccgaca aacggactcc agcggcaggc ttgataacaa
      241 cgtttcgtat ggcataaccc tgacacaact ggtgtatgac tttggtaaaa ctaacaacga
      301 tatcaatctg caaactgccg cccgtgacag ctaccgcttt aaattgatgg caaccttaac
      361 cgatgttgca gagaaaacgg cgactgccta catggaagtt agtcgttatc aggctttgtg
      421 cgatgcggct caacgcaata ttcactcgct ggaaaacgtc tacaacatgg cggcattgcg
      481 cgctaacgca ggcctgaact catcgtcgga tgaattacag gcccagacgc gtattgccgg
      541 aatgcgctca acgctggagc aatatcaggc gcagatggcg agcgccaaag cgcaactggc
      601 ggtgctcact ggcgtacagc cggaggcgat agccgcgcca cctgctgaat tagccgagca
      661 gccggtatcg ctgaagaata ttgattacca gtccatcccg ctggtgctgg cggcagaaaa
      721 cttacgccag tcagcacagt acggcgtgga aaaaacgaaa gcgcaatact ggccaacgct
      781 cagtattcag gggggtaaaa cgcgctacca gaccagcgac cgctcgtatt gggatgatca
      841 gctacaactg aacgttaacg cgccgctgta tcagggcggc gcggtttctg cccaggtgca
      901 acaggccgaa gggcaacaaa aaatctctgc ctcgcaggtc gaacaggcaa aactggatgt
      961 gctgcaacga gcgtctgtgg catatgcgaa ctggaccggc gcacggggtc gtgaagaagc
     1021 cggtttagcg caatccgaaa gtgcgcacaa aacgcgagat gtgtaccaaa atgaatataa
     1081 attgggtaaa cgcagtctga atgatctatt aaccgtcgaa caagatgtct ttcaggcgca
     1141 atcggctgaa ataaatgcca attatgatgg ctgggtcgcc gccgtaaatt atgccgctgc
     1201 ggtgaataac cttattccat tagcgggaat taaacagggc ttatacaacg acttacccga
     1261 cttgaaataa tcctgccatt attaatagtg ggccgttgtt aagtgattac ctttgtctta
     1321 tttagataaa tggactcgct aaggatgtgc gccatttatc aggaatgcca ttatgagcct
     1381 gattattgat gttatttcgc gtaaaacatc cgtcaaacaa acgctgatta atcctggcga
     1441 cgtcacggtt gttatttatg agccttccgt ggtgcaggtt catgctcagg cctctgccgt
     1501 tgcgcgttac gtccgtgaag gaaatgacct gctgatctat atgcgggacg gcacggtgat
     1561 ccgctgcaac ggttatttcc tgcaagcggc gaatacagct gaacaatcgg aactggtgtt
     1621 tgccgatggt caacagctaa cccatatcac ctttgccgat actgctgcgg gtggattagc
     1681 ccccgtagaa ctgactgccc agaccactgc gattgaaagc attgcgccat ttcttgatac
     1741 cgttgctcag accagcgcct tcccgtgggg ttggctggcg ggggcggcgg taggtggtgg
     1801 cgcgcttggt gcactgctgg caagcggtgg cgatggcgac tcgaaaacag aagtgattaa
     1861 taaccctacg ccacctgctg agcctggcaa cgccacacca tcatttttag ttaccgataa
     1921 tcagggcgat cagcgcggca ttctagccac caatgacatc accgatgaca ccacgccaac
     1981 ctttagcggc agcgggcagg cgggggcgac tattcagatt aaagacagta acggcaatac
     2041 tattgccagt actcaggtag acaacaacgg tcactggagt gtctcgttac ccacgcaaag
     2101 tgcaggtgaa catacctggt cagtggtgca aattgtcggc agtaccatca ctgacgccgg
     2161 ttcgataacg ttaaccatcg acaatagtca ggccagcgtg caggttgcca ccaccgcagg
     2221 cgataacatt attaacgcca gcgaacaggc cgccgggttt acgctttctg gcaccagtag
     2281 ccatctggcg cagggaacag aactcaccgt tacgctaaac ggcaaaacct acacgaccag
     2341 cgtaggcgct aacggtgcct ggagcgtgca ggtgccgacc gccgatgcac aggcgttagg
     2401 cgaagggaat caggcggtgc tggtcagtgg gaaagacgcc acaggcaata cggtcaccgg
     2461 cgcgcagcta ctaacggtcg atacccaacc gccaacgctt gccatcaaca ccatcgctca
     2521 ggacaacatt atcagtgctg cggaacataa cgtcgcgctg gtactgagcg gcacgtcgaa
     2581 tgcagaagcg gggcaaaccg taacactgac cgtcaacggg aaaagccata cagcaaccgt
     2641 cggtagcgac ggaacctggc aagtgacgct gcctgccacg gaagtccagg cactggcgga
     2701 gggtaattac gctgtcaatg ccagtgtcag cgatcgggca gggaacacca ccagccacag
     2761 cgcgaatttc acggtagaca cctcagcacc cgtggtcagt gttaataccg tggcgggcga
     2821 cgatattctt aataatgccg agcaggccgt cgcgcagatc atctccggac aagtcagcgg
     2881 tgcttctcca ggcgatacgg taacggtgaa attgggcact catgtcctga cgggcatcgt
     2941 gctggcagat ggcagctgga atgtggcgct ggacccagcg gtaacccgca cgctggatcg
     3001 cggagccaat acgattttcg tcaccgtgac agatgctgca ggaaatactg gcgcggcgtc
     3061 tcgagcaatc acgctggtcg gtgtttctcc gttgatcacc attaacaccg tctccggcga
     3121 tgacattatc agtggcgcag aaaaaggtgc gccactgacc cttaccggta gcactcaaca
     3181 ggctgagaca ggacaaaccg tcacagtaac cctggctgga cagagtttta ccactaccgt
     3241 gcaggccgat ggctcctgga gtctgacggt acctgccgcc gcgatgggaa atctgcctga
     3301 cggcgcggtg gcgattaccg cttctgtgac ggatctcagc ggcaataccg gcaacacttc
     3361 ccgcaccatt accgtcgata gccaggcccc ggccttaagc attgatccac tgaccgctga
     3421 taacatcatt aacgccgccg aaagcgggca ggatctgccc atcaccggca ccaccgacgc
     3481 tcagccgggg cagacggtga ccgttacgtt aaatgggcag acgtatcagg gcgtcgtgca
     3541 gccagacggc acctggagcg tgactgtgcc cgccgccaac gtgggcgcac tggctgacgg
     3601 caacgctacg gtcaccgcca gcgtgaacga tgtcgccggt aatccgagca gcgtttcacg
     3661 cgtggcgctg gtggatgcca cgccgccggt ggtaaccatt aatccggtgg cgaccgataa
     3721 cgtcatcaac acgccggaac atgctcaggc gcaaatcatc agcggcacgg ttactggcgc
     3781 tcaggcgggc gatatcgtca ccgtgacgct gaataatgtg gattacacca cggtggtgga
     3841 tggttccggc aactggagtc tgggcgttcc ggcctcggtg gtcagtgggc tggcggacgg
     3901 cagttatcct gtcagcgtct cggtaaccga caaagccgga aacacgggca gccagtcatt
     3961 gaccgtcacg gtcaataccg ccgcgcccct tatcggcatt aacagcattg cgggcgatga
     4021 tgtgattaac gccagcgaaa aaggggccga tctccagatt accggcacca gcgatcagcc
     4081 tgttaacacc gccatcaccg tgacgctgaa cgggcaaaat tacaccacca cgaccgacgc
     4141 ctccggcaac tggagcgtca ccgttccggc atcggcggtt acagcattag gccaggccaa
     4201 ctatacggta acggcggcgg tgaccagcga tatcggcaac agcgccactg ccagccataa
     4261 cgtgctggtc gacagcgcgc tgcccggtgt gaccattaat ccggtggcaa ccgacgatat
     4321 tattaacgcc gccgaagcgg gcgtggcgca aaccatcagc gggcaggtga ctggcgcgga
     4381 agatggcgac acggtaacta ttacgttggg tggtaatact tatacggcga cggtgggcag
     4441 caatctcacc tggagcgtgg acgttccagc ggcagatatt caggcgctgg gaaatggcga
     4501 tttaacggtt aatgcctcag tcaccaatca aaacggcaac accggcagcg gcacgcggga
     4561 tatcaccatc gacgccaatc tgcccggcct gcgggtcgat acggtggcgg gcgatgatgt
     4621 ggtcaatatc atcgagcacg ggcaggcgct ggtggtcacc ggcagcagct cggggctggc
     4681 tgaaagcacg ccgcttaccg ttacgattaa taatgtggaa tacaccactg cggtgcaggc
     4741 cgatggtagc tggagcgtgg gcgtcacggc ggcgcaggtt agcgcctggc ctgcggggac
     4801 ggttaatatt gccgtttcag gggaaagtag cgccggaaac tcggtgagca ttacgcatcc
     4861 ggtgacggtg gatctcactc cggcagcgat caccatcaac accatcgcca cggacgatgt
     4921 gattaacgcc gcagaaaaag gcgctgattt aaccctttcc ggcaccacca ctaacgtaga
     4981 acccggtcaa accgtcaccg tcacctttgg cgggaaaaat tacactgcca gcgtagcgag
     5041 cgatggtagc tggactgcca ccgtacccgc cgccgatctg gcgtcattac ccgagggcag
     5101 cgcctccgca ctggccagcg tcagcaatat caacggcaat agcgcctcgg cggtgcacaa
     5161 ctacagcgtc gacagcagcg cgccaaccat cattatcaat accgtcgcca gcgacaatat
     5221 cgtcaacgcc agcgaagccg atgcgggcgt gacggtgagc ggcagtacca ccgccgaagc
     5281 ggggcagatt gttacgataa cgcttaacag cccgaccgtg cagacctatc aggcaacggt
     5341 gcaggcggac ggcagctgga gcatcaatat tccggcggca gatcttgagg cattgaccga
     5401 tggcagccac accctgaccg ccacggtcaa tgacaaagcg ggcaatccgg cgagcaccac
     5461 gcataatctg gcggtggatc tcaccgttcc ggtgctgacc atcaacacca ttgcgggcga
     5521 tgacattatt aacgccaccg aacacgggca ggcgctggtg atttccggtt ccagcaccgg
     5581 cggagaagcg ggggatgtcg tcaccgtcac gctaaacagt aaaacctaca ccaccaccct
     5641 ggacgcctcc ggcaactgga gcgtcggcgt tccggcggcg gatgtcacgg cgcttggcag
     5701 cggcccgcaa actgtcaccg ccacggttac cgatgcggca ggcaacagcg acaattagac
     5761 acacacggtc accgttaacc tcaccgcacc aaccattgac atcaacacca tcgccaccga
     5821 tgacataatt aacgccagcg aaaaaggcgc ggatctgcaa atttccggca ccagtaatca
     5881 gccagcaggc accacgatta cgctggcgct taacgggcaa aattacaccg cgacgacgga
     5941 cgctgcaggc aactggagca ctacggtgcc agcttccgca gtaggtgcgc tgggcgaagc
     6001 cagctacacg gtgacggcga acgtcaccga cagcgcaggc aacagcaatt ccgccagcca
     6061 taacgtgcag gtcaataccg cgctgcctgg cgtcaccatt aacccagttg cgaccgacga
     6121 tattattaac gccgccgaat cgggcaatgc gcaaaccatc agcggccagg tgacgggggc
     6181 ggcggcgggc gatacggtta ccgtaacgct tggcgggaaa acttacaccg ctaccgtgca
     6241 ggggaattta agctggagcg tggacgttcc ggcggcggat attcaggcca tcggcaatgg
     6301 caatctgacg gttaacgctt cggtgaccaa cggcgttggc aatactggca gcggttcgcg
     6361 agatattact atcgacgcca acctgccagg tctgcgcgtt gacaccgtgg cgggcgatga
     6421 tgtggtcaat agcatcgagc acgctcaggc gctggtgata actggtagca gcagcgggct
     6481 ggcggcgggc gcggcgctga cggtggtgat taacacggtc acttacgctg caacagtatt
     6541 agccgatggc acatggagcg ttggtgttcc ggcggcagac gtgagtaact ggcctgcggg
     6601 tacggtgaat atcacggtaa gtggcactaa cacagccgga acaacatcca ccatcaccca
     6661 tccggtcacc gtcgatctgg cggcggtggc gatttccatt aacaccgttt ccggcgacga
     6721 tgtgattaac gccgccgaaa aaggggcaga tttaaccctt tccggcagca cctccggcgt
     6781 ggaagtgggg caaacggtca ccgttacctt tggcgggaaa acctacaccg ctaccgtagc
     6841 gggcgatggt agctggacaa ccaccgtacc cgccgccgat ctcagcgtgt tacgcgacgg
     6901 cgacgccacc gtgcaggcca gcgtcagcac tattaacggc aacacggctt cggcaaccca
     6961 cgcctacagc gtcgatgcca cggccccgac gcttgccatt aacaccatcg ccaccgacga
     7021 tattctgaac gctgccgagg cgggcaatcc gttaaccatc agcggtagca gcaccgccga
     7081 agcggggcag acggtaaccg tcacgcttaa tggtgtgact tacagcggct ccgtccaggc
     7141 ggacggcagc tggagcgtca gcttaccgac ggcggatctc agcaatctga ccgccagcca
     7201 gtacaccgtt agtgcctcgg taagcgataa agcgggtaac ccggcgtccg ctaaccacgg
     7261 gctggcggtg gatctcaccg tgccggtgct gaccatcaac accgtctccg gcgatgacat
     7321 tattaacgcc gccgaacacg gacaggcgct ggtgatctcc ggctccagca ctggcggcga
     7381 agcgggtgat gtcatcaccg tcacactaaa cagtaaaacc tacaccacca tgctggacgc
     7441 ttccggcaac tggagcgtcg gcgttccggc ggctgacgtc actgcgcttg gcagcggccc
     7501 gcaaactatc actgcggcaa ttaccgacgc ggcaggcaac agcgatgacg ccagccgcac
     7561 ggtcaccgtg aatctcgccg cgccaaccat tggtatcaac accatcgcca ccgatgacgt
     7621 gattaaagcc acggaaaaag gcgcggacct gcaaatcacc ggcaccagta atcagcctgc
     7681 gggcaccacc attacggtga cgctgaacgg gcaaaattac accgctacta ccgatagcaa
     7741 cggcaactgg agcgccacgg tgccagcgtc agcggttagc gcattgggtg aagccaacta
     7801 cacggtaacg gcaaacgtca ctgatacggc aggcaacagt aattccgcca gtcataatgt
     7861 gctggtcaac agcgccttgc ctgccgttac cattaacgcg gtggcgactg acgatattat
     7921 taacgctgcc gaatcgggaa atgcgcaaac catcagcggg caggtgacgg gcgcagcgca
     7981 gggggatacg gttaccgtta cgctgggcgg caacacctac accgccacgg tgcagtctaa
     8041 tttaagctgg agtgtggacg ttccggcggc ggatattcag gcgctgggca acggcgacct
     8101 gacggttaat gcctcggtca ccaatggcgt cggcaacacc ggcagcggct cgcgcgatat
     8161 cacgatcgac gccaatctgc ctggcctgcg ggtcgatacc gtggcgggcg atgatgttat
     8221 taacagcatt gagcacaatc aggcgctggt gatcaccggc agcagcagcg gattaacggc
     8281 gggaacggcg ttaacggtcg agattaacaa cgttacttat ggcgcgacgg tattagccga
     8341 cggcacgtgg agcctcggtg ttccggcggt agacgtcagc aactggccag cgggtacggt
     8401 gaatatcacg gtaagcggca ccaacagtgc cggaacaacc tccaccatta cccatccggt
     8461 taccgtcgat ctggctgggg tcgccatcac cattaacact ctctctggtg atgatgtgat
     8521 caacgccgtc gaaaaaggcg aaacgctggt cgtaagcggc agcaccagcg gtgtcgaagc
     8581 cgggcagacg gtgaccgtca cctttggcgg caaaaattac accaccacag tggaagctaa
     8641 cggtagctgg acggtgaatg tgccgcctgc cgatctcgct gcgctaccgg acggcgcggg
     8701 caacgtgcag gcgagtgtca gtaatattaa cggcaacagc gcccaggccg atcgcgcgta
     8761 tagcgttgat gccaccgcgc cgcttgtgac catcaacacc atcgccagcg acgatatcct
     8821 taacgtgagc gaagctggcg cggggatcac catcagcggc actaccacgg cgcaggccgg
     8881 gcagacgctc accgtcacgc tcaataacaa cacgtaccag accaccgttc tggcggatgg
     8941 cacctggagc gtgaatgttc cggcagcgga tttaagcgga ttaaccgcca gcagttacac
     9001 cgtgaccgcc acggtgagcg acaaagcggg taacccggca agcgccgacc acgcgctggt
     9061 ggtagatatc actgcgccgg atctcaccat taacaccgtc gcgggcgatg acattatcaa
     9121 cgccatcgaa catgatcagg cgttggtggt cagcggcacc agtacgggcg cggcggcggg
     9181 tgatgtggta accgtcacgc tgaacggtaa aaactacacc accacgctgg atgcctccgg
     9241 taactggagc gtgggcattc cggcggcgga tgtcacggcg ctggcgaccg gtagccagac
     9301 catcaccgcc agcctgagcg atcgcgcggg caacagcgac agcacgactc acgatgtgac
     9361 cgttgatctt agcggcccga cgctgaccat taacaccgtc tccggcgatg acattatcaa
     9421 cgccgctgaa atcgttgtgg cgcagaccat cagcggtcag gtcacgggaa cggcggttgc
     9481 cgggaatacg gtgattgtca ccattggcgg caatcaatat aacgccaccg tgcagtcaga
     9541 tttaagctgg agcgtcagcg taccggcgaa cgttttgcag gcgctgggta acggtgaact
     9601 gaccatcagc gcctcgttga ccaattccgc aaataatacc ggcaccgcga cgcacgatat
     9661 cgtgatagat gccaacctgc caggtctgcg cgtcgatacc gtggcaggcg atgatgtgat
     9721 taacagcatc gagcacactc aggcgttggt gatcaccggt agcagtagcg gactggcggc
     9781 gggcgcggcg ttgacggtgg ttatcaatag cgtcacctac ggcgcaacgg ttttagcgga
     9841 tggtagctgg agcgttggtg ttccggtggc agatgtcaca aactggcctg cggggacggt
     9901 caatattgcc gtctctggca ccaacaccgc cggaaccaca accagcatta gccatccggt
     9961 cacggtcgat ctcgctgccg tggcgatcac catcaacacc ctttccactg acgatgtgat
    10021 taacgccgcc gagaaaggct ctgatttgca gctctccggc accacctccg gcgtggaagc
    10081 ggggcaaacc atcaccgtta tcttcggcgg caaaagctac accaccacgg ttgccgcgga
    10141 taatacctgg gggctgacga tccctgccgt cgatgttgca accttgccag acggcgcagc
    10201 aaacgtccag gccagcgtca gcaatgtcgc agggaacagc acccaggcaa cgcatgctta
    10261 cagcgttgat gccactgcgc cctccgttac catcaacacc atcgccacgg acgatattct
    10321 taacgccgcc gaagcgggat cggcgctgac catcagcggc accagcacgg cggaagccgg
    10381 gcagacggtg accgtaacgc taaacggcgt taattacagc ggcaatgtgc aggcagacgg
    10441 gagctggagc gtcagcgtac caaccggcga tttagctagc cttaccgcca gctcgtatac
    10501 cgttaacgcc tcggtcagcg acaaagccag aaattcggct tcggcaacgc ataatctgac
    10561 ggtggacctt gccgctccgg tcgtcaccat caacacggtg gcgggcgatg acatcattaa
    10621 cgccacggaa cacggacagg cgcagatcat cagcggctcg gcaacgggcg cgactaccgg
    10681 taatacggtt tccgtgacga ttggcacgac cacctatacc accgtgctgg acgccaacgg
    10741 caactggagc atcggcgtgc ctgccagcgt gatttccgcg ctggcgcagg gcgatgtgac
    10801 cattaccgct acggtcaccg actccgcagg caacagcggc acggcctcgc acactgtcac
    10861 cgtggcgctc ggcgctccgg tgctcgccat taacaccatt gccgtcgatg acatcatcaa
    10921 cgccgcggag aaaggcgcgg atctggcgat taccggcacc agcaaccagc ctgcgggcac
    10981 gcagattacc gttacgctca acgggcaaaa ttacaccacc actgccgatg cttccggtaa
    11041 ctggagcgtg accgttccgg cgtcacgggt gagcgccctc ggtgaagcca cctacacggt
    11101 gaccgcagcc gccactgacg ccgatggcaa cagcggttcc gccagccata acgtacaggt
    11161 taataccgcg ctgccgggcg tcaccattaa cgtggtggca acggacgata ttattaacgc
    11221 cgccgaagcg ggcgtggaac agaccatcag cgggcaggtg acgggtgcgg cggcaggcga
    11281 cacggtgacc gtcacgctcg gcggggcgac ttacactgcc acggtgcagg cgaatttaag
    11341 ctggagcgtc gatgttccgg cctccgcgct acaggagttg ggcaacggcg aactgaccat
    11401 ttcggcttcg gtgacgaaca gcgtaggcaa tactggtaac ggcacgcgcg aaatcaccat
    11461 agacgcgaat ctccccggtc tgcgggtcga caccgtggcg ggcgatgatg tggttaatat
    11521 tatcgagcac gggcaggcgc tggtgattac cggcagcagc tccggtctgg cggcgggcag
    11581 caacgtcacg ctgaccatta acgggcaaac ctatgttgcg gcggtgctgg cggatggcac
    11641 ctggagcgtc ggcgttccgg cggtggatgt cagcgcctgg cctgcgggat cggtgacgat
    11701 tgcggcgagc ggtagcacct ctgccggaaa tccggtaagc gttacgcatc cggtgacggt
    11761 cgatctctcg gcggtggcgg tgagcatcaa cgccattacc gccgatgatg tgatcaacgc
    11821 tgccgaaaaa ggcgcggcgt taacgctctc cggcagcacc tctggcgttg aagccggaca
    11881 aacggttacc gtcacctttg gcggcaaaac ttacagcgcc acggtggctg cgaatggttc
    11941 ctggagcacc tcggttccgg cggcagatat ggcggctctg cgtgatggcg atgccagcgc
    12001 acaggccagc gtcagcaatg ttaacggcaa cagcgccacc acgacccacg cttacagcgt
    12061 tgatgccagc gcgccaacgg tgaccattaa taccattgcg ggcgatgata ttcttaacgc
    12121 cgccgaagcc ggagcggctc tgaccatcac cggcagcagc acggcggaag cggggcagac
    12181 ggtgaccgtc acgctcaatg gcacaaacta caccggcacc gtacagacgg acggcagctg
    12241 gagcgtcagc gtaccgtcag ccgacttaag caccctgacc gccagcaact acaccgtgaa
    12301 cgcggcggtg agcgacaaag ccggaaaccc ggcctcggtt aatcacaacc tgacggtgga
    12361 tacgtccgtt ccggtcgtca ccatcaacac ggtggcaggc gatgatgtga tcaacgcgac
    12421 ggaacacgcc caggcgcaga tcatcagcgg ctccgccact ggagcggcaa ccggtagcac
    12481 cgtgacggtg actatcggca caaatacctt taccacggtg ctggatgcca gcggcaactg
    12541 gagcgtcggc gttccggcaa gcgtcgtctc ggcactggcg aatggcacgg tgaccatcaa
    12601 tgccagcgtc accgatgccg gaggaaacag cggcagcgct acccatcagg tgacggtcaa
    12661 taccgggctg ccgaccatta cctttaacgc catcagcggc gataacatcc tgaacgccga
    12721 tgaaaaaggc cagccgttga ccatcagcgg cggcagtacg gggctggcga cgggcgcgca
    12781 ggtcaccgtc acgctcaacg gtcacaacta cagcgccacc accgacgcat cgggcaactg
    12841 gaccttaacc gtgccggtga gcgatctggc ggcattaggt caggccaact atacggtcag
    12901 cgccagcgcc accagtgcag caggcaacac cgccagcagc caggcgaatt tactggtcga
    12961 cagcggcctg ccggacgtca ccatcaacac cgtggcaggc gacgatatta tcaacgccgc
    13021 cgaagcgggg gccgatcaaa ccatcagcgg ggtggtgact cgcgccgccg ctggcgatac
    13081 ggtcaccgtg acgctgggcg ggaacactta caccgctacg gtacagagca acttaagctg
    13141 gagcgtcagc gttccgacag ccgatctcca ggcgttgggc aatggtgatt tgaccattac
    13201 cgcctcggtc accaacgcta atggcaacac cgggagcggc acgcgggata tcaccattga
    13261 tgccaacctg ccggggctgc gcgtagatac cgtggcgggc gatgatatcg tcaacagcat
    13321 cgagcacggg caggcgctgg tgatcaccgg cggcagtagc ggcctgaatg caggtgctgt
    13381 gctgacggtt accatcaaca gtgtggcgta ttccgccacg gtgcaggcgg acggaagctg
    13441 gagcgttggc attccggcgg caaacgtcag cgcctggcct gcggggccgt taaccgtgga
    13501 ggtagacggg caaagcagcg ccaataaccc agtcagcgtc agccatccgt tcaccgtcga
    13561 tttaacggcg gtggcaatca gcatcaacac cgttgccagc gacgacgtga ttaacgccgc
    13621 agaaaaaggc accaatctga ctctttccgg cagtaccagc gggattgaga gcgggcaaac
    13681 cgtcaccgtc acttttggcg gtaaaaccta cactgcaagc gtcgccgcca acgggagctg
    13741 gagtgtaaac gttccggcgg cagatctggc aactctgcca gagggcgcgg cgaatgtgca
    13801 ggccagcgtt agcagcgcga gcggtaacag tgcctcggcg acccatgcgt atagcgttga
    13861 cgccagcgcg ccgacgctca ccattaacac catcgccagc gacgatatcc ttaacgccgc
    13921 agaagccgga agcccgctca ccatcagcgg caccagcacc gccgaaaccg ggcagacggt
    13981 gaccgtcacc cttaacggcg caacctacac cggcactgtg caggcggacg gtagctggag
    14041 cgtcagcgtt cccacttcag ccctgggcgc gctcaacgca agcaattaca ccgtcagcgc
    14101 cacggtcaat gacaaagcgg gcaaccccgg cagcgccagc cataatctgg cggtagacac
    14161 caccgcgccg gttctcacca ttaacaccgt ggcgggcgat gacatcatca acgatgccga
    14221 acatgcgcag gcgctggtga tctccggcac cagtagcggc ggggaagcgg gcgatgtggt
    14281 gagcgttgtg ctcaacggca aaacctacac caccaccctg gatgcctccg gcaactggag
    14341 cgttggcgtt ccggcggcag atgttacggc gctgggtagc ggtgcgcaga ccatcaccgc
    14401 cagcgtcagc gatcgggcag gcaacagcga cgacgccagc cgcaccgtga ccgtcagcct
    14461 cagcgcgcca gtgattagca tcaacaccat cgcgggcgat gatgtgatca acgcgacgga
    14521 aaaaggatct gatctggcgc tttctggcac cagcgatcag cctgcgggta cggcgatcac
    14581 cgtcaccctg aacggacaaa actacagcgc caccacggat gcctccggca actggagtgt
    14641 taccgtgcct gcctcggcgg tcagtgcgct gggcgaagcg acctacagcg tgacggcgag
    14701 cgtcaccaat gctcagggta acagcagcac cgccagccat aacgtgcagg ttaataccgc
    14761 gctgccgggc atcaccatta atccggtggc gacggacgat attatcaacg cttcagaggc
    14821 aggcagcgcg caaactatca gcggccaggt aaccggtgcg gcggcgggca gcaccgtcac
    14881 cgttgaactg ggcggtaaaa cttacaccgc caccgtccag gctgatttaa gctggaatgt
    14941 gagcgtacct gccgccgact ggcaggctct gggtaacggt gagttaacgg ttaatgcctc
    15001 ggtgaccaac gccgttggca acaccggtag cggcacgcgc gatatcacca tcgatgccag
    15061 tctgcctggc ctgcgggtgg ataccgtcgc gggtgatgat gtggtcaata tcatcgaaca
    15121 cgctcaggcg caggtgatca ccggcagcag ctccggcttt gccgcaggca cggcgttgac
    15181 ggtggtgatt aacaaccaaa cttacgccgc cacggtgttg gcgaacggta gctggagcgt
    15241 cggcgttcct gcgacggatg tcagtaactg gcctgcggga acgctgaata ttaccgttag
    15301 cggggcgaac agtgccggaa cgcaaaccag cattacccat ccgctgactg ttgatctcac
    15361 cgccgtcgcc atcagcatga acagcatcac cagcgatgat gcgattaacg ccgccgaaaa
    15421 aggcgcggcg ttaacgctct ccggcagcac gtccggcgtc gaagcggggc aaaccgtcac
    15481 cgtcaccttt ggcggcaaaa cctacaccac cacggtggcg gcaaacggta gctggagcac
    15541 caccgttccg gcggcggatt tggcggccct gcgtgatggc gatgccagcg cccaggtgcg
    15601 ggtgactaac gtcaacggca acagcgccac agcaacgcac gaatacagcg tcgatagcgc
    15661 cgcgccaacg gtgaccatca acaccattgc cagcgataac atcatcaacg ccagcgaagc
    15721 ggcggcgggc gtaacggtgt ccggcaccag caccgcgcaa accgggcaga cgctcaccgt
    15781 cacgcttaac ggcactaact accagaccac ggtgcagaca gacggcagct ggagtttaac
    15841 gctgcccgcc agcgacttaa ccgcacttgc taataacggc tacaccctga ccgccacggt
    15901 cagcgatctg gcgggtaacc ttggcagcgc cagcaaaggc gtgaccgtcg ataccactgc
    15961 gccggtgatc agttttaaca ccgtggcggg cgatgatgtg attaacaacg tcgaacacat
    16021 tcaggcgcag attatcagcg gcaccgccac gggcgcggtg gcgggcgacc gcctggtggt
    16081 gaccatcgcc gggcagcagt atgtcaccag caccgatgcc agcggcaact ggagcgtcgg
    16141 cgtgcctgcc agcgtgattt ccggccttgc ggatggcacg gtgaccatca gcgccaccat
    16201 taccgatagc gcaggcaaca gcagcacgca gacgcacaac gtacaggtga acacggcggc
    16261 agtgtcgctc tcggtcagca ctatcagcgg cgataacctt attaacgccg ccgaagcggg
    16321 cagtgcgctg accctgagcg gcactggcac taatttcgcg acaggtacag tggtgaccgt
    16381 gttgcttaac ggcaaaggct acagcgccac cattcagagc aacgggagct ggagcgtgaa
    16441 cgtgcctgcg gcggatgttg cggcactcag tgatggcacc agctacacgg ttagcgcctc
    16501 cgctcaggac agtgccggaa acagcgccac ggcctcgcgc agcgtggcgg tggatctcac
    16561 cgcgccggtt atcagcatta acaccgtttc gacggacgac cgcctcaacg ccgccgaaca
    16621 gcagcagccg ttaaccctta acggctcgac cagcgcggaa gtggggcaga cggtcaccgt
    16681 cacctttggc ggcaaaacct ataccgccac ggttgccgcc aatggtacct gggcgctgaa
    16741 cgtgcctgcg gtggatctgg ccgcgctcgg gcagggcgcg cagaccatta ccgccagcgt
    16801 gaacgatcgc gccggtaacc ccggacaggc cacgcacgcc ctgaccgtcg ataccgttgc
    16861 gccaacggtc accatcgcca cggtggcggg tgacgatatt atcaacaacg ccgagcagct
    16921 tgccgggcag accattagcg gcaccaccac cgccgaagtg ggccagacgg tgaccgttac
    16981 ctttaacggg caaacctgga gcgcaacggt tggcagcggc ggaagctggt cggtgtttat
    17041 tccggcgcag cagtttgccg gattaagcga cggcagctac accattagcg cgacggttag
    17101 cgatcaggcc ggaaaccccg gcagcgccag ccgtggcgtg acgctgaacg gcgatgtacc
    17161 tactgtcacc atcaacacct ttgcgggcga cgatgtggtg aatgcagcgg aacacggctc
    17221 atcgctggtg atcagcggca ccaccaccgc gcccgtcggg cagacgctga ccctgacctt
    17281 aaacggcaaa acctacacca ccacggtgca gacgggcggt agctggagct acaccctcgg
    17341 cagcgccgat gtcaccgcgc tggcggacgg caacgcctac gtgattaacg cctcggtgag
    17401 caatgccatt ggcaacaccg gtagcagtaa tcacaccatt accgtcgatc tcagcgctcc
    17461 ggcgatgggc attaatatcg attccctgca agccgacact ggccttagcg ccagcgactt
    17521 tatcaccagc gtcagtccgg tagtggtcaa cggctcgctc accgccgcgc ttgccagtaa
    17581 cgagacggcg caaataagta tcgacggcgg cacaacctgg accacgctta ccgttaccgg
    17641 cacgacctgg cgctataacg acagccgcac gctgaccgat ggcaactatc tctatcaggt
    17701 gcgggtgatt gacgcagcgg gcaacgttgg cgcgaccgac agccagaatg tggtgatcga
    17761 cactaccgcg ccagatcccg cggtgaaaac catcgccatc agcgcgatca ccaccgatat
    17821 gggcttgatc actaacgatt ttgtcaccag cgacacgacg cttgcggtga gcggcacgct
    17881 gggggcgacg ctttctgccg gtgagttcgc gcaaatcagc ctcgacggcg gcgtcacctg
    17941 gactacgtta accgtcgttg gcaccagctg gagctatgcc gatggtcaca cgcttactga
    18001 cggcacctgg aactacacgg tgcgggtggt ggatctggcg gggaacgttg ggcagaccgc
    18061 aacgcaaaac gtggtggtcg acaccaccag cccggaagcg gcgaaaagta tcaccattac
    18121 cggtatcagc gatgacaccg gaaccagcag cagcgatttt attaccagcg acaccacgct
    18181 taccgtgcgc ggcgtattgg gcgcggcgct cggcgctaat gagttcgcgc aaatcagtac
    18241 cgacaacggc gcaacctggg tgaacgtgac cgtcgccgca gacagcctga actggagtta
    18301 cgttgacgga cgaaccctca ccaacggcac caccacctgg caggtgcggg tggtcgatct
    18361 ggcgggcaac gttggcgcaa cgagcagcca gtcggcgctg atcgataccg ttaacccggc
    18421 gcaggtgctc accatcgcca gcatcagcac cgacacgggg agttcggcaa ctgactttat
    18481 caccagcgac accatgctca cgctgaccgg ttcgctgggg gcggggcttg ccagcggcga
    18541 agtggcgcag attagccttg atagcggcgc gacctggaca acgctcacca ccaacggtac
    18601 acagtggact tacaccgaca gccgcacgct gaccgacggc agctacgttt atcaggtgcg
    18661 ggtgctggag ctggcgggga acaccggccc ggtggtgtcg aaaacggtgg tggtcgatac
    18721 gattaacccc accgccacac caacgattgt gtcgtatacc gatgatgtcg ggcagcggca
    18781 ggggacatta agcagttcgc aggccaccga cgacactacg ccgctgctga acggtgtact
    18841 ttccgcgccg cttgccagcg gtgaagtggt ttacctctac cgtaacgggc tgctgttagg
    18901 ggcggtgacg atggtcggcg ctctgaactg gacctacagc gacagcgggc tggtgagcgg
    18961 tgcctatacc tacagcgcgc gagtggtgga tttggcgggg aatatcacct cctccagtga
    19021 ttttgtcctg acggtcgata cctctattcc gaccacgctg gcgcaaatca ccagccagac
    19081 cacgcgcgat actatagcgg ggtgatcacc gccgcgcttg ccagtgggca gtatgttgaa
    19141 gtggtgatca acggcaaaac ctacacctct gaaccgggcg gcgcggtagt ggtcgatccg
    19201 gcgcacaaca cctggtatgt acagttgccg gataccgatg cgctgacagt ttccgcgacc
    19261 gcctataccg ttactgcgca ggtaaaaagt tcggcgggta acggcaataa cgccaatatt
    19321 agcaacggca cggtgacggt taacgcggcg attgattaca caccgacctg gactaccgcc
    19381 agcaaaacca ccgcctgggg gctgacctac ggcctcgact cgcacgggat gtggacggtg
    19441 ctggcaaacc agcaggtaat gcaatcgact gacccactca cctggtcgaa gaccgcgctg
    19501 acgctgtatc agagcggcaa caactacgcc accagctcca ttgccgatta cgaccgtaac
    19561 ggcacgggcg atctgtttat cacccgtgat gactacggta cgggctatat taacggcttt
    19621 accaataacg gcgatggcac cttttccagc gctattcagg tcaccgtcgg caccctgacg
    19681 tggtacggct cgattgtggc atttgataaa gagggcgacg gctatctcga cttctggatt
    19741 ggtgacgctg gcgggccgga ctccaacacc ttcctgtgga acaacgcagg cacgctggta
    19801 ggcaactcca ccacgtcgaa cagcggcggt agcgccacgg tgggcggggc ggtgacgggg
    19861 tatctttcgc tcaacgaagg ttctggcgtc gatctgaaca atgacggcag gatcgacctg
    19921 gttcagcaca cctataacct gaacaactat tacacgctgt cttcgctcat caaccagggg
    19981 aatgggacgt ttgtctgggg gcagaacacc accaatacct tcctgagcgg ggcgggcagt
    20041 ggcgctatga gcagcagcgt ttccatgacc tgggccgatt tcgatggtga cggcgatatg
    20101 gatctcttcc tgcccgccag ccagggaaga gctaactacg gctcgctgtt attcaacacc
    20161 aacggcgtac tgggtagccc ggtggcggtg ggcgcaacgg caaccaccta cgccagccag
    20221 tttagcctgg cggtggactg gaaccacgac ggcctgatgg atatcgcccg tatcgcccag
    20281 accgggcagt cgtatcttta tactaacgtc agcaacgcca gcaactggac gcaatcggcc
    20341 ctcggcggca gccagagcgg taccaccagc ggcgtggcgg caatggacta cgactgggac
    20401 ggcgcggtgg atgtgctggt gtccaaacag tcgggcagcg tgttcctgag ccgcaacacc
    20461 aacacggtga gctacggcac ttcgctacac ctgcgcatca ccgatcccaa cggcattaac
    20521 gtctattacg gcaataccgt gaagctgtac aactcggcgg gagtgctggt cgccacgcaa
    20581 atcatcaacc cgcagtcggg tatgggggtt aacgacacct cggcgctggt caacttctac
    20641 gggctgaatg ccggagaaac ctacaacgcg gtgctgatca aatccaccgg caccaccgcc
    20701 agcaatatcg accagacggt caacaccagc tggggcggtt tacaggccac cgatgccact
    20761 cacgcttacg atctcagcgc tgaagcgggt accgcgagca acaacggcaa gttcgtcggc
    20821 accggctata acgacacctt cttcgccacc gcaggcaccg acacttacga cggttccggc
    20881 ggctgggtgt acagctccgg caccggaacg tggctggcga acggcgggat ggacgtggtt
    20941 gatttccggc tttcgacggt gggcgtgacg gctaacttaa gcagcactgc cgcgcaggcc
    21001 accggtttta acacctcgac atttaccaat atcgaaggca tttccggctc gaattttaac
    21061 gacattctga ccggcagcag cggcgataac caactggaag ggcgcggcgg caacgacacg
    21121 ctcaacatcg gcaacggcgg ccacgacacc ttgctctata aactgctcaa cgccagcgac
    21181 gccactggcg gcaacggctc agacgtggtg aatggcttta cggtagggac atgggaaggc
    21241 accgccgaca ccgatcgcat tgatattcgt gaactgttac agggcagcgg ctacaccggc
    21301 aacggcaaag ccagctacgt caacggcgtg gcaacgctgg atgcgcaggc cggaaacatc
    21361 ggtgactttg tcaaagtcac ccagagcggc agcgacacca tcgtgcagat cgaccgcgac
    21421 ggcacgggcg gcacttttgc gacaactaac gtggtcacgc tgacgggcgt gcacaccgac
    21481 ctcgccacct tgctggcgaa tcatcagttg atggtggtgt agataatgaa aaaaaatgcc
    21541 cagtccgtgg aagcctggct ggaggcgatg atcgccgtgg cgcgctatta tcggctcgat
    21601 ttttctcagg agaatgtgcg cgcgaccgtc aactgggagc gcgacagtaa gcgggaggag
    21661 ttactcaccg acatggcgcg gcagttaggg atggggttgc ggttggtgga gttttccgcc
    21721 gattcgctta acccctggcg tctgccgctg atcgcggtgt ttgataatca gcagattggc
    21781 gtgatcaccc gtcgcgacaa tcatgacaat atcagcgttc agttcagcgg tgacgaaggg
    21841 ctggaaacta cacttaacgt cgcggatatt gaagataaaa tcgtcgaact ggcgctgctg
    21901 cgccctctca gcgccatccc tgacgcgcgg gtggatgact atattcgccc gtatcaggcg
    21961 aactggttct ggagtctttc gctaaaagac tggcggcgct acggcgatat tatgctggcg
    22021 tcactggtgg cgaacgtgct ggcgctggcg gcgatgattt tctcgatgca ggtttacgat
    22081 cgggtggttc cggcgcaatc gtacccgacg ctatgggtgc ttttcgccgg ggtgatgatg
    22141 gcgatcctgt ttgagttctg tatgcgcatg gtgcgtacgc atctatctga tgtgatcgga
    22201 aagcgggcag acctgcgtat ttcggatcgc gtttttggtc atgcgctacg gctgaaaaac
    22261 aacgtgcgat cgaaatccac cggatcgttt atctcgcaga tccgcgaact ggaatcagtg
    22321 cgggagctta ttacgtccac caccatcggc gcggtggcgg acctgccatt cttcctgctg
    22381 tttgtcttta ttttgtggat gatcggcggc tggctggtgc tggtggtttt gctggcgctg
    22441 ctgttgctgg tgatccccgg cctgctggtg caacgcccgc tggcgcggct ggcgaacgaa
    22501 ggaatgcgcg agtcagcggt acgcaacgct acgctggtgg aagccgtgca gtcgattgaa
    22561 gatattaaac tgctgcgcgc cgaacagcgt tttcagaatc agtggaacca caccaacgac
    22621 gtggcctcgt ccatcagcat gaagcaacgt tttctcaccg ggctgctgct cacctggacc
    22681 caggaagtgc agtccatcgt ctacgtggtg gtgctgctgg tgggctgctt tatggtgatg
    22741 aacggcgaca tgaccaccgg cgcgctggtc gggacgacga tcctggcttc gcgcaccatt
    22801 gcgccgctgt cgcagatatc cggcgtgctc tcgcgctggc agcaggcgaa agtagcacgt
    22861 aacggccttg atgaactgat gaagcgcccg gtggatcagc cggaacacgg caaactggtg
    22921 cataaagcgg tgctgcacgg taattatcag ttcagcaacg cggtgtttta ttacgacgaa
    22981 gaagagaaga ttgccgatgt agcgattggc aaactcaaca ttcaggctgg tgagaaaatc
    23041 gccattctcg ggcgcaacgg cgcgggtaaa tcgacgctac tgcaaatgct cgccggaatg
    23101 cgtatcgccc agcaggggca ggtgttgctg gataacatca gtatcgggca gcttgatccg
    23161 gcagatctgc ggcgagacat ggggctactc agccagaccg ggcgactgtt ctttggttcg
    23221 ctgcgggaaa acctcaccat ggggatgccg gaagccagcg atgaagatat cgaacgcgcg
    23281 ttaaccctca gcggagcgct gccctttgtg cagaagcaga aaaacggcct caactacatg
    23341 attcaggaag ggggatttgg cctctctggt ggtcagcggc aaacgctgct gctggcgcgg
    23401 ctactcatct ctcagccgaa tatcgtcctg ctcgacgaac ccagcgcctc actggacgaa
    23461 atggcagaag cgtatttaat cgaacaactg aaacagtgga ttgggcatcg cacgctgatt
    23521 atcgccactc accgcacggc gatgctgcaa ctggtggata ggataatcgt gatggatcag
    23581 gggcggattg tgatggacgg cgcgaaagag gccattttgc gtgaacaggg cgagcccaca
    23641 gcgcgacggg tggtgttgca ggagaagaat aaggggtctg cggcatgaac ggacaactgc
    23701 aatataaccg tggtctgact gaaccccgcc tgccgcgttc ggcgctggcg gtacgcgtta
    23761 ccgctgtgat gctgctctgt tttctcggct gggcgtggta cttccagctt gatgaagtca
    23821 ccaccggcag cggcacggta gaaccttccg ggcgcgaaca ggtggtgcag tcgctggaag
    23881 gcggaattct gtatcatctc gacgtgaaag ttggcgatat tgtcgaacag ggccagccgc
    23941 tggcgcagct taaccgcacc aaaacggaat ccgacgtcca ggaagcgatg tcgcggctgt
    24001 acgcggcgct ggcaacctcg gcgcggctgc gggcggaagt gagcaataaa ccgctggtct
    24061 tccctgacga actgaataaa ttccccgaac ttatcgagtc ggaaacggcg ttgtacaaca
    24121 ctcgccgcga cgggttgaac aaagccacca ccgggctgac gcaggggatt agcctggtca
    24181 atcgcgaact ggcgatgacc cagccgctgg taaaacaggg ggcagcgagc agcgtcgaag
    24241 tactgcgcct gcaacgccag gccaatgaac tggaaaacaa actcagcgac gtgcgcacgc
    24301 aatactacgt tcaggcgcgc gaagagttag caaaagccaa tgcggaagtg gaaacccagc
    24361 gttcggtgat ccgtggacgg gaagactctc tcaccaggct caactttacc gcgccggtgc
    24421 gaggcattgt tcaggatatt gatgtcacca cggttggcgg cgtcatcgcc cccggtggta
    24481 aactgatgac catcgtgccg ctggatgagc aactgctgat tgaggcaaaa atctccccgc
    24541 gcgatgtggc gtttatccac ccggggcaaa aatcgctggt gaaaataact gcctacgatt
    24601 actccatcta cggcggcctg ccgggagaag tggcggtgat atcaccagac accgtgcagg
    24661 atgaagtgcg gcgcgatgtt tattactatc gggtgtacat tcgcactttc agcaaccatc
    24721 tggaaaacaa aagcaaacag cagtttccga tattccccgg tatggtggcg acggtcgata
    24781 tccgcaccgg caaaaaaagc gtgctggatt atctgttgaa accctttaac aaagcacagg
    24841 aggcgttgcg cgaacgatga acattagcga tgtagcaaaa attaccggcc tgaccagcaa
    24901 agccattcgc ttctatgaag agaaggggct ggtgacgccg ccgatgcgca gcgaaaatgg
    24961 ttatcgaacc tacacgcagc agcatctcaa cgaattgacc ttactgcgcc aggcgcgcca
    25021 ggtgggcttt aacctggaag agagcggcga gctggtgaat ctgtttaacg acccgcagcg
    25081 tcacagtgcc gacgtcaaac gtcgcacgct ggagaaggtg gcggagattg aacggcatat
    25141 cgaagaactg caatctatgc gcaaccagct actggcgctg gcgaatgcct gccccggtga
    25201 tgacagcgcc gactgcccga ttatcgaaaa tctctccggc tgctgtcatc atcgggcggg
    25261 gtgaaaatca gtgaatcccc gtttgcaccg aaagaatatg ctgcaaactt tctaccataa
    25321 tgcgccccga ctgaaataag ccttcctgga tttcgttgtt aacattcaca gcgtgaacca
    25381 gggaggctaa taatactact tgcaagaagg caaaaataaa cgcaccgata atcataatat
    25441 gcgtcagggg tattttcctt tgctccttca ttttaaataa cccacaaact ggccttcttt
    25501 cattaaggcg acttttcctg tgtgatgata aacaatgacg ttatatagac ctgactgata
    25561 aacagtggca tctttattgt cttcatcaat tgcacataat gaatcattga gataagcaaa
    25621 gtttttactg ctcattctaa aggtcatgcc aaatccttta taaatggctt tctttgccgc
    25681 ctgggcgtgt tgtgcggctt ctgcattttt aaatgcctgt gcctcggatg cacttaaaat
    25741 agccgcctga cgctgctgtt ctgcgaggta acaggtgtca tgactgacgc cctgcgcctg
    25801 gcaatctgcg atgcgggaag tgggtgaact acagccagcc agtaatataa ccgccagcag
    25861 ggaaaacgat ttaatggagc tattcatcat tgttaacctt aaataatgta caaattaatg
    25921 aagaggtgaa cccggcaagc ttccttgctc ttatttatat tgaggattga aatattagaa
    25981 cgaatatccc acgccgacac ctgcgccgaa atcagattgt gtatccgcag aaataccggc
    26041 tttaagtacc gtatgcgttc cggcattaaa tgatgttccg acagacaccg cagattcacc
    26101 gttgaaggtt cccgctcccg ctgacaccat catcacgtca ccggttttaa cctgtggttg
    26161 tgaggcgata gcgatggcag atgcagttcc cgcattggct tctttacgat tgtcatcaac
    26221 ttcgtttttt agcgaactga aatggttatt gagcgtgtta atttgctggc tgttatctgc
    26281 aacccgtttt tgggtgttgg ttaactgttg gtgcgtttgg gttacgtctt gtgacaggct
    26341 gtcaatttgt tcatgtgcct gggcagactc ctgcgccaga ctttgcattt gtttatcgta
    26401 ttgagtcctt tcattttgct gttgtactgc cacatcggtt gtcagcgcat tcattttctg
    26461 cccttgttca gtggcggtgc gctgattagc ctcattttga gccgccagtc tggcattaat
    26521 catttcctga taatgcgcgg tattttgcgt atctccagtc gcggcgagcg ttttttgtgt
    26581 ggctacgatc tgcgcctgtt gctctgtaat ttgcgtttgc tgggcgtcag ttaccgcaga
    26641 ctgtcgggcg ttgatcatgc tctgataacg ggcagttgtc tgtacatcac ctgtagcggc
    26701 aacggttttt tgtcctgcgt caggatctgt ctggacagtg ggagtctgag tggtatcagg
    26761 tgtttcagaa gggatgtagt gatgttcagt caaataggta tacgcagcgg ggctgttgtc
    26821 tgtgcgttct aaaaagcggg taatcttctg ctcaatctcc gattgcgaca tgccagagtt
    26881 aatggctgaa agctctgttg ccgccgcttc aatggtatca ccagctaaaa caacagggct
    26941 ggatgttgct gaaattatga gtgccagtaa agctacgttt acagttttca ttcattttct
    27001 cttgttca
//
LOCUS       DBFIFI010000048        26932 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.048, whole genome shotgun sequence.
ACCESSION   DBFIFI010000048 DBFIFI010000000
VERSION     DBFIFI010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26932
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.048"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(151..1014)
                     /gene="potB"
                     /locus_tag="N7B39_001890"
     CDS             complement(151..1014)
                     /gene="potB"
                     /locus_tag="N7B39_001890"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HGU2421883.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLARDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKATEIDN"
     gene            complement(998..2134)
                     /gene="potA"
                     /locus_tag="N7B39_001891"
     CDS             complement(998..2134)
                     /gene="potA"
                     /locus_tag="N7B39_001891"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HGU2421884.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            2384..3610
                     /gene="pepT"
                     /locus_tag="N7B39_001892"
     CDS             2384..3610
                     /gene="pepT"
                     /locus_tag="N7B39_001892"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HGU2421885.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMAMLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            complement(3659..4780)
                     /gene="roxA"
                     /locus_tag="N7B39_001893"
                     /gene_synonym="ycfD"
     CDS             complement(3659..4780)
                     /gene="roxA"
                     /locus_tag="N7B39_001893"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HGU2421886.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYTNGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            5029..6258
                     /locus_tag="N7B39_001894"
     CDS             5029..6258
                     /locus_tag="N7B39_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU2421887.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFITQLVEQNRHTGLLLGEVKQEASVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            6623..6811
                     /locus_tag="N7B39_001895"
     CDS             6623..6811
                     /locus_tag="N7B39_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU2421888.1"
                     /translation="MLSTDRFIREKECEKLTGLSRTCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            6869..7423
                     /locus_tag="N7B39_001896"
                     /pseudo
     CDS             6869..7423
                     /locus_tag="N7B39_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
     gene            7616..7813
                     /locus_tag="N7B39_001897"
     CDS             7616..7813
                     /locus_tag="N7B39_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HGU2421889.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARN
                     RCPSGCVAVFAARIRQEVRHD"
     gene            7806..8018
                     /locus_tag="N7B39_001898"
     CDS             7806..8018
                     /locus_tag="N7B39_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2421890.1"
                     /translation="MINLSLTDLNRIQFREKFTGQLLVNVDKGRVVCNYHLPDEAIVA
                     TRESLQEITKRAGMINTNIQGMLYAQ"
     gene            8008..8472
                     /locus_tag="N7B39_001899"
     CDS             8008..8472
                     /locus_tag="N7B39_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2421891.1"
                     /translation="MHNKTTPDAAAEAIKTLMHALIDISVIADRAHKHATSETEYAGA
                     FVPHSLAVMQFSADTALNEAKAILIADCENGGVMRDDRFNSLKQEFSGVPDDAADALS
                     SMPELIRAAFFLLSTREYKSTGLDVLNIAADYAEYVAEARYRRKFPEDVSHA"
     gene            8465..8698
                     /locus_tag="N7B39_001900"
     CDS             8465..8698
                     /locus_tag="N7B39_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2421892.1"
                     /translation="MRDIYHQTIDRAFLALSHSENMMEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            8704..9003
                     /locus_tag="N7B39_001901"
     CDS             8704..9003
                     /locus_tag="N7B39_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HGU2421893.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGTVMIKGVEERRVTY
                     RREGYEYDCVMPVYQFRRDFSLVQTAPHNVPTSNARARANIQKLKTMVNGFRGKK"
     gene            9000..10400
                     /locus_tag="N7B39_001902"
     CDS             9000..10400
                     /locus_tag="N7B39_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="HGU2421894.1"
                     /translation="MKSAPNLKKQPYDKMTEVIIFAGSDAWAHAKQWQEQDGRLAGDN
                     VPPVVLADDQLDELAELRIIDEGRYCVRLYKAGHIRPSNINAIAHKLAAAGVTDANYY
                     PEGMHSHMRENWREYLERVRGKEPAEEKNHQRKTTLPMSVGSTGYDTQLDYVVKGIIP
                     AVSLCSIYGASGSYKSFLAGSWACHVATGRQWGGRRVAHGAVLYVVGEGGIGVPRRVK
                     AWEVVHDEQVKNLYLVNRPIFPAAPLDVDEMVIAARQVERETGKPVRMIILDTLARCF
                     GGNDENDSRDMGAFIRGCDELKRRTGATVLVVHHSGKDETKGARGSSAFRASLDAEYR
                     IRREDAGSEALVISCTKMKDAEELKEAAYDLRVVELFTDADGELITSLVVVDDPRPPV
                     ELERIEEAGNKTENHTALWGCIRSRTQNGDKCTIPLLRDDMKKLGYEMKNFRRWLYKL
                     EKDGVIRIDGDDVAPL"
     gene            10601..10852
                     /locus_tag="N7B39_001903"
     CDS             10601..10852
                     /locus_tag="N7B39_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2421895.1"
                     /translation="MTQKRRDRTEPKYKALDMTEHTLKVAIRTIDRHTREGYAKEHPD
                     LISAFMTTAAANFATLTEREIAEAEQVTTINVKTGEVEL"
     gene            10849..11259
                     /locus_tag="N7B39_001904"
     CDS             10849..11259
                     /locus_tag="N7B39_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HGU2421896.1"
                     /translation="MTAQIAAYGRLVDDPQVKQTSKGTPMTLARMAVSLPCSQAQDGQ
                     ATLWLSVIAFGKQAGFLAKHQKGDVASVSGTMQVSQWTGQNGETRQGWQVIADSVISA
                     RAARPGGNRRKTTGTQGNQPPAGGDDPYGDDISF"
     gene            11270..11542
                     /locus_tag="N7B39_001905"
     CDS             11270..11542
                     /locus_tag="N7B39_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HGU2421897.1"
                     /translation="MVHDRIAEELEAKGFYRRAAARWGEVMQLVETDKERHHITMRRL
                     ECSRKAQRAPEPPDNFGDLKKAVDRTYAEMGIDGAGDEIWRNYQDS"
     gene            11499..11627
                     /locus_tag="N7B39_001906"
     CDS             11499..11627
                     /locus_tag="N7B39_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001301569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HGU2421898.1"
                     /translation="MVLVMKYGAITRTANQTAGEIRLFLHQLKRYGALPGFRHGQA"
     gene            11669..11893
                     /locus_tag="N7B39_001907"
     CDS             11669..11893
                     /locus_tag="N7B39_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2421899.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMSINDYRAFLETGALIEKDQHG
                     FVRCALSGEMLAVNPEQIDALIEFLKEIRD"
     gene            12145..12351
                     /locus_tag="N7B39_001908"
     CDS             12145..12351
                     /locus_tag="N7B39_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2421900.1"
                     /translation="MAEEKKSGVSVYISPEIVEVLKQRHKKNYEAGVAAGLDPLMTPE
                     PSIGSLVRSYLLAALGMHKNYGGE"
     gene            12351..13406
                     /locus_tag="N7B39_001909"
     CDS             12351..13406
                     /locus_tag="N7B39_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HGU2421901.1"
                     /translation="MAGKATALNTNQLFMYLNRGDIADFKFSPLFTTLFFPNVATFST
                     QNIMLDTLDIEEVTMSAFCSPMVGSQVQRDKGYETSTIKPGYMKPKHEIDPTKTIMRM
                     AGEDPAQLNDPTYRRMRLITGNMRRQINAIKARVEWLAVNAVTTGKNIIEGEGIERYE
                     IDWKIPENCIIEQAKGKKWSEQDKDMHDPIYDIELYADQAGCPANVMIMGAEVWRTLR
                     SFKKFRELYDLSRGSESAAELACKNLGEVVSFKGYLGDLALIVYSGKYTDSDGTEKYF
                     LEPDLLVLGNTNNKGLVAYGAIMDQEAVRTGATQNMFYPKNWIEDGDPAIEYVQTHSA
                     PQPVPADIRKFVTVKIG"
     gene            13419..13754
                     /locus_tag="N7B39_001910"
     CDS             13419..13754
                     /locus_tag="N7B39_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HGU2421902.1"
                     /translation="MDTPYIELFAGSQQVSTTLVHFAADAGVIQEFTPLMLADNGEFK
                     PWDGQESGKAVYLTSYPVDTSKQKSAQCYKTGIFNIAAVNWPESVDTDAKKCAAFAGS
                     GVSVQPLAR"
     gene            13767..14180
                     /locus_tag="N7B39_001911"
     CDS             13767..14180
                     /locus_tag="N7B39_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HGU2421903.1"
                     /translation="MATNESIMTLPLASKFKVEARAMADRGLSTYEAVYQLNKLEEQD
                     KPCADAIMALHEHKDYQPLLRAMANVPCIDVGTAKSILSMTIEQERPKVAPELTAAFE
                     NFMDMHSPQAVSAGMAYDGRNQGDDGDIDRILKTI"
     gene            14386..14928
                     /locus_tag="N7B39_001912"
     CDS             14386..14928
                     /locus_tag="N7B39_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HGU2421904.1"
                     /translation="MKSHLMNKKNMAKSCRVSATAFDKWGVTPVERKGREAFYDVASV
                     IENRVNNAISQLINDKGEIDDDELLRVRIRLLTAQAEAQELKNDRDRGDVIDTEFCLY
                     ALSKLASQISSIMDSLPLTMQRSFPQITPAMLDRLKREVVKACNASARVADNLPQILA
                     DYLKETTGNVPEKLLQKKGE"
     gene            15184..15465
                     /locus_tag="N7B39_001913"
     CDS             15184..15465
                     /locus_tag="N7B39_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2421905.1"
                     /translation="MTEAELLGLIRRVAGISQQHDEQATQPDSVTAENYVRVVAEVMR
                     RDGIELNGVDMRNIRNRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(16066..17526)
                     /gene="phoQ"
                     /locus_tag="N7B39_001914"
     CDS             complement(16066..17526)
                     /gene="phoQ"
                     /locus_tag="N7B39_001914"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HGU2421906.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMHGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITKQYEGKIVAGESMLGGARMEVIFGRQHSTPKDE"
     gene            complement(17526..18197)
                     /gene="phoP"
                     /locus_tag="N7B39_001915"
     CDS             complement(17526..18197)
                     /gene="phoP"
                     /locus_tag="N7B39_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HGU2421907.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLICRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(18366..19736)
                     /gene="purB"
                     /locus_tag="N7B39_001916"
     CDS             complement(18366..19736)
                     /gene="purB"
                     /locus_tag="N7B39_001916"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HGU2421908.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(19740..20381)
                     /gene="hflD"
                     /locus_tag="N7B39_001917"
     CDS             complement(19740..20381)
                     /gene="hflD"
                     /locus_tag="N7B39_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HGU2421909.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLKRKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(20417..21523)
                     /gene="mnmA"
                     /locus_tag="N7B39_001918"
                     /gene_synonym="trmU"
     CDS             complement(20417..21523)
                     /gene="mnmA"
                     /locus_tag="N7B39_001918"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HGU2421910.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILIVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPI"
     gene            complement(21577..22038)
                     /gene="nudJ"
                     /locus_tag="N7B39_001919"
     CDS             complement(21577..22038)
                     /gene="nudJ"
                     /locus_tag="N7B39_001919"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HGU2421911.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(22048..22701)
                     /gene="rluE"
                     /locus_tag="N7B39_001920"
     CDS             complement(22048..22701)
                     /gene="rluE"
                     /locus_tag="N7B39_001920"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309634.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HGU2421912.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREATE"
     gene            22873..24123
                     /gene="icd"
                     /locus_tag="N7B39_001921"
     CDS             22873..24123
                     /gene="icd"
                     /locus_tag="N7B39_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HGU2421913.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYELAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            complement(24237..25379)
                     /locus_tag="N7B39_001922"
     CDS             complement(24237..25379)
                     /locus_tag="N7B39_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HGU2421914.1"
                     /translation="MAASPRSHKISIPNLYCKLDKRTGKVYWQYKHPLSGRFHSLGTD
                     ENEAKQVATEANTIIAEQRTRQILSVNERLERMKGRRSDITVTEWLDKYNSIQEDRLQ
                     HNELRPNSYRQKGKPIRLFREHCGMQHLKDITALDIAEIIDAVKAEGHNRMAQVVRMV
                     LIDVFKEAQHAGHVPPGFNPAQATKQPRNRVNRQRLSLPEWQAIFDSVSRRQPYLKCG
                     MLLALVTGQRLSDICNLKFSDIWDDMLHITQEKTGSKLAIPLNLKCDALNITLREVIS
                     QCRDAVVSKYLVHYRHTTSQANRGDQVSANTLTTAFKKAREKCGIKWEQGTAPTFHEQ
                     RSLSERLYREQGLDTQKLLGHKSRKMTDRYNDDRGKDWIIVDIKTA"
     gene            complement(25369..25605)
                     /locus_tag="N7B39_001923"
     CDS             complement(25369..25605)
                     /locus_tag="N7B39_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HGU2421915.1"
                     /translation="MSRLITLRDWAKEEFGDLAPSERVLKKYAQGKMMAPPAIKVGRY
                     WMIDRNSRFVGTLAEPQLPINANPKLQRIIADGC"
     gene            25745..26533
                     /locus_tag="N7B39_001924"
                     /pseudo
     CDS             25745..26533
                     /locus_tag="N7B39_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247981.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
     gene            26530..>26932
                     /locus_tag="N7B39_001925"
     CDS             26530..>26932
                     /locus_tag="N7B39_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HGU2421916.1"
                     /translation="MKNIAAQMVNFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVIAGLPNVSELVDMVYEYC"
BASE COUNT         7103 a         6406 c         6920 g         6503 t
ORIGIN      
        1 tgttttttta tagcgatttg ctaaaaggga gaagaaaaaa cgttctccaa aattagattg
       61 caactgtttg attttatgga tagaggcatg ctgtttacta gacagtaaaa agcatgccaa
      121 atttcctatt aaccttttga atataaagca ctaattatct atttctgttg ccttcttatt
      181 cagcagacga gaagcgcgcc agtaaaccag caacatcagg cccattacga tagtcagcgt
      241 aatgctggtg gctgcaccaa acggccagtc acgaatattg aggaactgta ccttgatgac
      301 gttaccgatc agcaggtttt tcgcaccgcc catcaggtcg gacacataga acaggcccat
      361 cgctggcagc atcaccagca gacatccggc aataatcccc ggcatcgtca gtggaataat
      421 gatgcggata aaagtctgta acttgctggc accgagatcg cgcgccgcct caagcaacgg
      481 cttatccagt ttttcgatac tggagtacag cggcatcacc ataaacggta gcagaatgta
      541 aaccagaccg ataatcaccg cactgggcgt gaacatgata cgaattggtg tgtcgataac
      601 gcccagccag agcaaaaact cgttgagata gcctttggtg ctgaggaaaa ttttcagccc
      661 gtagatgcga atcagtgagt tggtccagaa gggaacaatc agcagaaaca gcagcagcgg
      721 acgcaccttg tgtggcagct tcgccagaaa ccaggcaaac ggatagccca gcaccaggca
      781 ggcgagggtg gcgatcagcg ccatattcag cgagtgcaat agcacttcaa aatagagcgg
      841 atcgagcaga cgtgtgtagt tatccagcgt aaagaccatt ttgacaaaac tggcgtcgtc
      901 gcgggccaaa aagctggtgc caatgatcat caggttgggc agaaagacaa acaacacaag
      961 ccaaccgaca atagtgacaa tcactacatt ctggaactta cttgtgttct tcatcagcca
     1021 gtacgacctc ccagctttct acccaattaa tggccatttt ttggtcgaga gagtggtcaa
     1081 agtcaggatc gtcttcattg aagaattcgc tgaccatcac catcttgcca ttttccagtt
     1141 caacaaccga ctccagcgtc atgcctttgt agttacgctc gcgaacgtaa ccaatcagcc
     1201 cttcagcgtg gttgtcgtcg ttaatctctt caacacgtaa gtcttccggg cgcagcagaa
     1261 catgcagttt ttgccccggt tcaacggcga agttaacgta gatattacat tcgcggcctt
     1321 caacgttggc gcgtacgcgc tgctcgtcga gacgttcgat gacagtggcg ttaaacatat
     1381 tgatttcgcc aatgaacccg gcaacaaaca ggtttttcgg ctcttcgtag atttcacgcg
     1441 gcgtgccgtc ttgctcaatg cgaccatcgc gcatcaccac aatcctgtct gacatggtga
     1501 gtgcttcttc ctgatcgtga gtcacgaaga cgaatgtaat gccaagctta cgctgtaacg
     1561 ctttcagctc gttctgcatt tgcttacgca gtttgtaatc cagcgctgag agcgactcat
     1621 ccagcaacaa cagacgaggc ttgttaacca ccgcgcgagc aatggcgacg cgttgctgtt
     1681 gaccaccaga gagctgatgc ggtttacgtt gagcgaaggt ttccagctgc accatccgca
     1741 gggcttccat cacgcggggc gtaatttcag cagcgggggt tttttgcatg cgcaacccaa
     1801 aggccacatt ttcgaacacg gtcatgtggg ggaaaagtgc gtagctttgg aaaacagtgt
     1861 tcacatagcg gttttccgcc ggaacgtggg tgatgtcctc gttatccaac atgatgcgtc
     1921 cggaatcaac agtttccaga cctgcaatca ggcgaagaac ggttgtttta ccgcagccag
     1981 aagggccaag cagcgtgagg aactcgccat tgttgatagt cagatccagc tggggaatga
     2041 cctctttacc atcaaagcat ttgcgaattc ccgccaattg caccagcggt gaaagcgaac
     2101 tcggttgttt attcaatttt ttactctgtc ccatgtaaac gcaacggatg gcttaccgat
     2161 gcggggtttg tggttaacca ccttggtgac tcttaatgag ggcggtaatt ctacggcaaa
     2221 ccgcttgaat cgccaatctt tgttgtgaat tactggctta gctttatatt cattaaggta
     2281 atgctgataa atattcccgc ttgcaggggt aaaagtgacc tgacgcaata tttgtctttt
     2341 cttgcttctt aataatgttg tcacaaaaag tgagggtgac tacatggata aactacttga
     2401 gcgatttttg aactacgtgt ctctggatac ccaatcaaaa gcaggggtga gacaggttcc
     2461 cagcacggaa ggccaatgga agttattgca tctgctgaaa gagcagctcg aagagatggg
     2521 gcttatcaat gtgaccttaa gtgagaaggg cactttgatg gcgatgttac cggctaacgt
     2581 ccctggcgat atcccggcga ttggctttat ttctcatgtg gatacctcac cggattgcag
     2641 cggcaaaaat gtgaatccgc aaattgttga aaactatcgc ggtggcgata ttgcgctggg
     2701 tatcggcgat gaagttttat caccggttat gttcccggtg ctgcatcagc tactgggtca
     2761 gacgctgatt accaccgatg gtaaaacctt gttaggtgcc gatgacaaag caggtattgc
     2821 agaaatcatg accgcgctgg cggtattgca acagaaaaac attccgcatg gtgatattcg
     2881 cgtcgccttt accccggatg aagaagtggg caaaggggcg aaacattttg atgttgatgc
     2941 cttcgatgcc cgctgggctt acactgttga tggtggtggc gtaggcgaac tggagtttga
     3001 aaacttcaac gccgcatcgg tcaatatcaa aattgtcggt aacaatgttc atccgggcac
     3061 ggcgaaagga gtgatggtaa atgcgctgtc gctggcggca cgtattcatg cggaagttcc
     3121 ggcggatgaa agcccggaaa tgacagaagg ctatgaaggt ttctatcacc tggcgagcat
     3181 gaaaggcacc gttgaacggg ccgatatgca ctacatcatc cgtgatttcg accgtaaaca
     3241 gtttgaagcg cgtaaacgta aaatgatgga gatcgccaaa aaagtgggca aagggttaca
     3301 tcctgattgc tacattgaat tggtgattga agacagttac tacaatatgc gcgagaaagt
     3361 ggttgagcat ccgcatattc tcgatatcgc ccagcaggcg atgcgtgact gcgatattga
     3421 accggaactg aaaccgatcc gcggcggtac cgacggcgcg cagttgtcgt ttatgggatt
     3481 accgtgcccg aacctgttca ctggcggtta caactatcat ggtaagcatg agtttgtgac
     3541 tctggaaggt atggaaaaag cggtgcaggt gatcgtccgt attgccgagt taacggcgca
     3601 acggaagtaa gcgaaaaggg atgcggcatg tgatgccgca tccggcttaa atccaaactt
     3661 acccttcgaa gaaccaatac ccgctattga ccagcgccgc gagcatcgcg aggaatgacg
     3721 gatcttccag cgcatcgcca aaattctccg cagtcagcgc aatgttgctg gcgagtgcat
     3781 ccagtgccgg acggtgcggg gaatcgatct tctcaccatt ggtatacacg tcgtcgccaa
     3841 tgcgcaatac gcgcagacca cccaggcgca ccagcacatc accttgtttc agcgcatcgt
     3901 agatttcatc tggctgataa ggcggctccg gcggcgcgat atccagttca tgacgtgact
     3961 gggatataaa ctcgccaaac cattgcttaa agtgttccgg ctggttgatc aattcgagca
     4021 tcatctcacg cagtttatcc atctcttgcg gcagaacgtc cgcaggatga gcgcgaggtg
     4081 gaacatcagg atcgctgtag tagttgccgc ccagttcgcg ttgcagcaca taatcggcaa
     4141 atccgctaat taattcccgc gtatttggcg cgcgaaaacc cacggaatag ttcatcgcat
     4201 tttccagcgc gtagccttca tgcgggaatc ctggcggaat ataaagaata tcgccaggct
     4261 ccagctcttc atcgatgatg gcttcgaacg gatcgacctg taacagatcc ggatgtgggc
     4321 agtgctgttt catttgcagc ttctcgccca ctcgccagcg acgacgtccg gtaccctgaa
     4381 tgataaacac gtcgtactga tcgagatgcg ggccgacgcc gccgccaggt acagaaaaag
     4441 agatcatcag atcatcaata cgccagtccg gtagttcacg gaacggtcgc atcagcgcgg
     4501 cggtcggctc atgccagtgg ttcactgctt gcactaacaa tgaccagtta gtttcaccga
     4561 gatgatcgta gctttcgaac ggaccgtggc tgacctgcca ttttccatcc tggtgactga
     4621 ccagtcgact gtcgacttcg ctttccatcg ccagacccgc caactcgtct ggagagatcg
     4681 ggtcaataaa attattaaag ccgcgtttta acaccaccgg gcgtttctgc cagtgacgtt
     4741 caagaaaatc gggccagtta agagtgagtt ggtattccat gttaagcatc cacaggctgg
     4801 tatctgcaac cgattataac ggatgcttaa cgtaatgcgt gaagtatgga catattttat
     4861 caattagtgt tttttgccgt ttcataatgt ttcagattgt tcattatatt tttgattata
     4921 aacagatttg tgattttcct tgttccagct tgtttcacgt tgtagccctt tgtgcaacga
     4981 tcggtgtagt taatggtgta gttaattgtg atgaccggaa tcaaaattat gagcagagca
     5041 cttaacaaac tgagcgatac acagctgagg aaaatcaacg gcacacccgc ccaaaaaaca
     5101 gcctttctta atgacggtgg aaacctgagc gtcaggcatt caaccagtgg ccttttaacc
     5161 tggtatttca cttacagggc cggaacggga aggggggcac caccggaacg cattaagctg
     5221 ggaaattatc ctgatctgag cctgaaatca gccagggaaa aagccgccca gtgtcgcgca
     5281 tggctggcag aggggaaaaa tccacgtcat gagcttaatt acaccgtaca ggaagcgtta
     5341 aagccggtaa cggttggcga tgcgctcacc tactggcttg agtcgtacgc aaaggaaaac
     5401 cgcgtggatt atgccgccct gaaaaagcgc cttaataatc acgtaataca gcacattggt
     5461 gctatgccgc tggataaatg cgagctacgg cactggctgg cctgttttga ccaggtggca
     5521 aagcgaacgc ctgttactgc cggattcttg ctacagacgt gcaaacaggc gcttaagttc
     5581 tgccggaggc ggcgctatgc aatcagcaac gttcttgatg atatgagtgt ggcggacgtt
     5641 gggaaaaaac cggatataag cgagcgtgtc ttaagcacca aagaactggg cgaattattg
     5701 caggcactgg acaaaaaaat attctccccc tactacatcg cgttaatccg cctcctgatt
     5761 gtgttcggat gccggacggt agaactgagg ttatcggaga tcagcgagtg ggattttacc
     5821 gaaatgctct ggaccgttcc gaaggagcac agcaaaacga aggtagccat attccggcct
     5881 ataccggaag caatactgcc gttcatcacg cagctggtgg agcagaacag gcacacgggc
     5941 ttattgctgg gggaagtgaa acaggaggcc agcgtatcgc agtatggaag attagcgcac
     6001 aggaggctta atcaccctca ctggtcactg catgacatcc ggcgcacctt tacaaccatg
     6061 ctgaacgatt taggcgttga tccgcatgtc gtggagcagc ttacaggcca ccagatgcca
     6121 ggaatgcagc gagtttataa tcattcccgt tatctggatg cgaaacgcaa tgcgctggat
     6181 atgtggacgg agcggttagg gatactggcg ggaacacatg aaaacgtaac cacgctacca
     6241 gtagccagaa gaaaataatt tttttcgtgt tttttcagta tgcgcatact ggatatgcga
     6301 acagatacaa cgtgcaacaa tgagcaacaa tgtggaacaa ctacgaacaa gaggcgaaaa
     6361 agcgtacgga tttataagta gctgattttt aatgtgttac tggtttttta tacactagcg
     6421 aatcactctt taaagagcga gaaaaaaggc atggaacaat attttatgcc tttgtattaa
     6481 catgaattta aaagatttta ttcttacttt ttcattttct gtaaatcata catcctcagt
     6541 atttgccatt catcactcta gcgggaacaa tacgacacaa taagacacct gatgactctt
     6601 taagaaacga aagggggcaa tagtgttaag cactgatcgg tttatacgtg aaaaagaatg
     6661 cgaaaaacta accggcctta gccgtacgtg tcgctaccgc ctggaaaagg ccggacaatt
     6721 cccatcacgt cgtaaacttg gcggtcgttc cgttggctgg tctttatccg aggttctggc
     6781 ctggaaggat agctgcaagg cagttcatta atcacgctgg cggcacacag ccgccacaca
     6841 tcaatcatct gaacacagag ctataaccat gaagattgaa tatacgccag aacgtgggcg
     6901 gggattcgtt cgccctggtg agactggaaa accacaaaat tggggttttt caggcataaa
     6961 aaaagcggcc ccgaaatgga gccgcctttc tgaacagata acccgctgcg ccttatgtgt
     7021 atatgatccc aaacataagc acggggatga tagccgctat caggctggtg ggcaatgcta
     7081 tcagtctggt tcagttcgtt gccatacctg caatgggcgc gtttccctgt actctttaag
     7141 gaattgctca agggcaaaag cacatggcgc gaatctttct gattcatgct ctatctttct
     7201 gcgccgtctt ttccgtgccg gtgataatgt tttggtcaat tctttatcgg tcattgtgtt
     7261 gtcctgcata gcaatgcgcc gtaatacctc acaccacggc gctgatagtt tttatccttt
     7321 gggttctatg ccgcgttgct gtaattcttt gcggagaacg cgtttaatcc aagtggctaa
     7381 agatgtgtcg ccatcggcct tagcagcttc ttctagctgc gctctgaagt cttccggtaa
     7441 tcgcatttgg tatggaggcg aacgtttttc taatggtgtt gacatggtaa ttacccttta
     7501 ttgctatacc tacatggtaa ttaccattgt acttgccatt acacaaaaat caacgccccg
     7561 cagtgctggg aacacatacg gggcgtctaa ccaacaacgt aaactaggag ccgttatggt
     7621 tgccgtaaat catataccac accttgtaca cacacaaacg gcctttgtgt ggcgttttct
     7681 ggcactgagt gccggagaat ctcaaatcat ccacgtaacc gcctggacgg aacgcgaagc
     7741 gcgtaaccgt tgcccgtccg gttgtgttgc tgtatttgcc gcccgtattc gtcaggaggt
     7801 gcgccatgat taatctgtca cttactgacc ttaaccgcat tcagtttcgt gagaaattca
     7861 cagggcagct actggtcaat gtggataaag gtcgcgtggt gtgtaattac cacttaccag
     7921 atgaagcaat tgtcgccaca agggaatcat tacaggaaat cacaaaacgt gccggaatga
     7981 ttaacacgaa tattcagggg atgctttatg cacaataaaa ccacaccgga cgcagccgcc
     8041 gaagccatca aaacgctgat gcacgcgctg attgatattt ctgttatcgc ggacagggca
     8101 cataagcacg ccaccagcga aacagaatat gccggtgctt tcgttcctca ctcactggct
     8161 gtcatgcaat ttagtgctga tacggcgctg aatgaggcca aagctatcct gattgctgat
     8221 tgtgaaaatg ggggggttat gcgtgatgat cgttttaatt ccctgaaaca ggaattttcc
     8281 ggcgttcctg atgatgcggc tgatgcgctt tcgtcaatgc cagaacttat tagagcggct
     8341 tttttcttac tttccacgag agaatataaa tcaacggggc ttgatgtact gaatatcgcc
     8401 gccgattatg cggaatatgt ggcagaggcg cgttatagaa gaaaatttcc tgaggatgta
     8461 agccatgcgt gatatttacc accaaacaat agaccgcgca tttcttgcac tttctcacag
     8521 tgaaaacatg atggaaatat tgcgcatatg gcttgaaaca cttggcgaca atgaacgcga
     8581 caaacaaaaa tcaagaattg ccacggcatt aataacgctt cttgagcctg taataatgga
     8641 actgcaagaa atagatctat tgcacgacag atataaagaa cagcacaccg gagaataaaa
     8701 ataatgaaac ttaaatattc tggcttaact gccagtggca acactcaccc taaatttacg
     8761 cgcggtgata tttaccgcga ccagtacggc ggcacggtaa tgattaaggg cgtggaagaa
     8821 cggcgcgtaa cctaccgccg tgaaggttac gaatatgatt gcgtgatgcc tgtttatcag
     8881 ttccggcgtg atttttctct ggtacagacc gcgccgcata acgtgcccac cagcaacgcc
     8941 agggcacggg caaacatcca gaagctgaaa accatggtta acggattcag gggcaagaaa
     9001 tgaaaagcgc accgaactta aaaaaacagc cttacgacaa gatgaccgaa gtcattattt
     9061 ttgcgggtag tgatgcctgg gcacatgcga aacagtggca ggaacaggac gggcgactgg
     9121 ctggcgataa tgtgcctccc gttgtgctgg ctgatgatca actggatgaa ctggcagaac
     9181 tgagaatcat cgacgagggg cgctattgtg tccggctgta caaggcaggc cacatcaggc
     9241 catcaaatat taatgccatt gcgcacaagc tggcggcggc gggtgtaact gatgcgaatt
     9301 attaccccga agggatgcac agccatatgc gggagaactg gcgcgaatac ctggaacggg
     9361 tgcgcgggaa agagccggcg gaagaaaaaa accaccagcg aaaaaccacg ctaccgatga
     9421 gcgttggatc taccggatac gacacgcaac tggattacgt ggttaagggg attattccgg
     9481 cggtatcgct atgcagcata tacggggcta gcgggtccta taaatcattc cttgccggat
     9541 cgtgggcgtg ccatgttgcc actggtcgcc agtggggagg ccgcagggtt gcacatggtg
     9601 cggttctcta tgtggttggt gaaggcggta taggtgttcc gcgtcgtgta aaagcctggg
     9661 aggttgtgca cgatgagcag gtgaaaaatc tgtatctggt aaaccgcccc atctttccgg
     9721 ctgccccgct tgatgttgat gaaatggtta tcgctgcccg tcaggtggag cgggaaacgg
     9781 gtaaacctgt acgcatgatt attctggata cgctggcgcg ttgctttggt gggaatgatg
     9841 aaaatgattc ccgtgatatg ggggcgttta tccgtggttg tgacgaactg aaacgacgca
     9901 caggggccac ggtgctggtg gttcaccatt ccggcaagga tgagacgaaa ggcgcgcgcg
     9961 gttccagtgc atttcgtgct tcgctggatg ctgaataccg gatacgcagg gaggacgcag
    10021 gaagcgaagc gctggttatc tcatgcacca aaatgaagga cgcggaggaa ctcaaagaag
    10081 ccgcatatga cttacgcgtg gtggagcttt ttaccgacgc tgacggtgaa ttaatcacgt
    10141 cgctggtggt ggtggatgat ccgcgccctc ctgttgaact ggagcgcatc gaggaggcag
    10201 ggaacaagac ggaaaaccat accgcgctat gggggtgcat ccgttcacgc acacagaacg
    10261 gcgacaagtg cacgatcccg ctgttacgtg atgacatgaa aaagctgggg tatgaaatga
    10321 aaaacttccg gcgctggctg tacaagctgg aaaaagatgg ggttattcgt atcgatgggg
    10381 atgatgtagc gccgctataa aagtgaggag caaaagcgag ggggatagaa agagggccaa
    10441 aattagcccg ctctccctca cttttcgacc tgtatacatc ctcaaaagtg aggggtaaaa
    10501 aaatacttat gaaacacaca catagaaaaa ccgaaaatcc caactgcgac gaagtgagac
    10561 gcttgaaaaa gtgaggcgaa aaagtgagag gttgcgagaa atgacccaaa aacgcagaga
    10621 cagaacagag ccaaaatata aagcgttaga catgactgag cacaccttaa aggtggcaat
    10681 cagaacgata gaccgccaca cgcgggaagg atacgcgaag gaacatcccg acctgataag
    10741 cgcattcatg accacggcgg cggcaaactt tgccacgctg acagaacggg agattgccga
    10801 agcggaacag gtaacaacca tcaacgttaa aaccggagag gtggaattat gacggcacag
    10861 atagccgctt acgggcggct ggtggacgac ccgcaggtaa aacagaccag caagggcaca
    10921 ccgatgacgc tggcgcgtat ggcggtatct ttgccatgca gccaggcaca ggacggacag
    10981 gcgacgttat ggttatcggt catcgcattt ggcaaacagg ccggcttcct ggctaaacat
    11041 caaaaaggcg atgttgccag cgtatccggc acgatgcagg taagccagtg gaccggacag
    11101 aacggagaaa cgcggcaggg ctggcaggtt atcgcagaca gcgtaatcag tgcccgcgcg
    11161 gcacgtccag gcgggaacag acgcaaaacc acaggcacac agggtaatca gccaccagcg
    11221 ggaggcgatg acccttacgg tgatgatatt tcgttctgag ggggtgacga tggtacatga
    11281 ccgcatagcg gaggaactgg aggcgaaagg cttttaccgg agggcggcgg cgcgatgggg
    11341 tgaagtcatg cagctggtgg agacagacaa ggaacggcat cacatcacga tgcgacggct
    11401 ggaatgttca aggaaggcac agagggcacc ggagccgccg gataatttcg gagacctgaa
    11461 aaaggcagtc gatcgcactt atgccgaaat gggtatagat ggtgctggtg atgaaatatg
    11521 gcgcaattac caggacagct aatcaaacag ccggagaaat ccggcttttt ttgcaccagt
    11581 tgaaacggta tggcgcatta ccgggttttc gtcacggtca ggcatagtta ctatctgaaa
    11641 caaacagaca caacagagga aaaaaacaat gccgatgaaa tttgatgaga tattaaaaca
    11701 gcgtgataaa taccatgctg acaacatgga gacgatgagc atcaatgatt accgcgcatt
    11761 cctggagacg ggcgcactga ttgaaaagga tcagcatggt tttgtgagat gtgctctatc
    11821 cggtgaaatg ctggcggtaa atcctgaaca gatagatgca ttgatagaat ttctgaaaga
    11881 gatcagagac tgagccagca cacagcacac atagccggag caatccggct tttttgcgcc
    11941 caaaaaaagc ccgataaggt cagagggttc ttatcgggct tttgcatatg aggtttttgg
    12001 atgcactggc gttcgtgatc gggataatca tttcataatt tgcaacataa ctcaatatca
    12061 ttgcataaaa tgcaattctg attataatca ggactggata aacatccagt tatgattttt
    12121 ttagtcgaag aggaatttct tactatggct gaagagaaaa aaagcggtgt ttcggtgtac
    12181 ataagccccg aaatcgtgga ggtgctcaag cagcgccaca aaaaaaacta tgaagctggc
    12241 gttgcggctg gactggatcc gctgatgacg ccggagccgt cgataggttc acttgtacgc
    12301 tcttatttac ttgcggcgct tgggatgcat aaaaattatg ggggtgaata atggcaggca
    12361 aagcaacggc acttaacact aaccagcttt tcatgtacct gaatcgcggg gatattgcgg
    12421 attttaaatt cagccctctg tttaccacgc tgtttttccc gaacgtggcg acattcagca
    12481 cgcaaaacat catgctggat accctggaca ttgaagaagt caccatgtcg gcgttttgtt
    12541 cgcctatggt gggtagccag gttcagcgcg ataaagggta cgaaaccagc acaatcaaac
    12601 ctggctacat gaagccaaag cacgaaatcg atccaacgaa aaccatcatg cgcatggctg
    12661 gagaagatcc ggcacagctt aacgacccta cctatcgccg tatgcgcctg attactggca
    12721 acatgcgccg ccagataaac gccattaagg cacgcgtgga atggctggcg gtgaatgcgg
    12781 taacgaccgg aaaaaacatc attgagggcg aaggcataga acgctatgaa atcgactgga
    12841 aaataccgga aaactgcatc atagagcagg ccaagggtaa aaaatggtcc gagcaggata
    12901 aagacatgca cgacccaatc tatgacatcg agctttatgc tgatcaggct ggttgccccg
    12961 caaacgtcat gattatgggc gctgaggtat ggcgcacatt acgcagcttt aaaaaattcc
    13021 gtgagctgta cgatctttcc cgtggttcag aatccgccgc cgaactggcc tgtaaaaacc
    13081 tgggcgaagt ggtgagcttt aaaggctatc ttggtgatct ggcccttatc gtctattccg
    13141 gcaaatacac tgacagcgat ggtaccgaaa aatatttcct tgagcctgat ttgctggtcc
    13201 tgggtaacac caacaataaa gggctggtgg cctatggtgc gataatggat caggaagcgg
    13261 taagaacggg cgcaacacaa aacatgtttt acccgaaaaa ctggattgag gacggcgatc
    13321 cggcgattga gtacgtgcag acacacagtg caccgcagcc ggtaccggca gatattcgca
    13381 aatttgttac cgtcaaaatt ggttaacggg gggattctat ggacactcca tacattgagt
    13441 tatttgcagg cagtcagcag gtatccacga cgttggtaca ttttgccgct gatgctggcg
    13501 ttattcagga atttaccccg ctgatgctgg cagacaatgg cgagtttaag ccgtgggatg
    13561 gtcaggaatc tggcaaggct gtttatctga cctcgtaccc tgtggacacg tcaaagcaga
    13621 aatcagcaca gtgttacaag acggggatat tcaatattgc cgccgttaac tggcctgaga
    13681 gcgtcgacac cgatgcgaaa aaatgcgccg cctttgcggg ttccggcgta tccgttcagc
    13741 cgctggcccg ataagcaggg ggaacgatgg caacgaatga aagcatcatg acgctaccgc
    13801 tggcgagtaa atttaaagtc gaagcgcggg caatggctga cagaggttta tcaacctatg
    13861 aggccgtata tcaactaaac aaactggaag agcaggacaa gccgtgcgct gatgcgatta
    13921 tggcgcttca tgagcataaa gactatcagc cgctgttgcg tgcaatggca aacgtgcctt
    13981 gtatcgatgt tggtacggct aaaagcatcc ttagcatgac catagagcag gaacgtccga
    14041 aggttgcgcc ggaacttacc gcagcctttg aaaactttat ggacatgcac agcccgcaag
    14101 ccgtatcagc tggcatggca tacgatggca gaaaccaggg cgatgacggc gacatcgatc
    14161 gcatactgaa aaccatctga gacaaggccg gagaaatccg gcttttttac gggtccttcc
    14221 tggaattatg gcccgttacg gggcggcgac ctcgcggatt ttcgctattt atgagccttt
    14281 tcagggtggt ggtggtggtt ttgttgtttg atctatcttt atgaatgaaa agggaaagat
    14341 gcaagcaata caccaacctg aagcagtaat taagttggtg tattaatgaa atctcatctg
    14401 atgaacaaaa aaaacatggc gaaaagctgc cgtgtaagtg cgacagcgtt cgacaagtgg
    14461 ggagtaactc ccgttgagcg taaaggccgt gaggcgtttt atgatgttgc cagcgtgata
    14521 gaaaataggg ttaacaatgc aattagccag cttataaacg acaaaggcga gattgacgat
    14581 gatgaacttt tacgagtcag gatcagatta ctgacagcac aggcagaggc gcaggaactt
    14641 aaaaacgatc gcgatcgcgg tgacgtgatt gatactgagt tttgcctgta cgcgctttca
    14701 aagctggcga gtcagatttc atcaatcatg gacagcctcc cgcttactat gcaaaggagc
    14761 ttcccacaga ttacccccgc catgctggac aggcttaaaa gggaagtggt taaagcctgt
    14821 aatgccagtg ccagagttgc cgacaacctc ccacagatac tggctgatta cttgaaagaa
    14881 acaaccggaa acgtaccgga aaagttgcta cagaagaaag gcgagtaaca gacgcgcaat
    14941 tattgaacaa aaatgagaaa ggatctgata atggcatttt ctggctaaaa tgcctcgtgt
    15001 cagatccttt ctttttttaa tattttcatt gttaaacagt ctgttacaga gaagaagatc
    15061 ggatctgact tttgccggaa aattctcata aacagataaa aaccgcgagg tcgccgcccc
    15121 gtaacgggcc ataattccag gaaggacccg acgacaccag actatcagag cgatgggggc
    15181 acaatgacag aagccgaact actgggatta atccgccgcg tcgccggaat cagccagcag
    15241 catgacgaac aggccacaca gccggacagc gtgaccgctg aaaattatgt gcgtgttgta
    15301 gcggaagtga tgcgccgtga tggtatagaa cttaatggcg ttgatatgcg caacatacgg
    15361 aaccgcgttc ttgaaatgct ggcctacaat cgccgcgttg aactgtatcg ggagaaagaa
    15421 aaaataacgt accactggaa gaagccggaa cggctgcggc ggtaacttgc tgatattccc
    15481 gataacgcaa aattgcgctg gctacatcct cacatctgag gactgttaat cagaaggccg
    15541 gaatatattc acaaactacg aaattttcgt agtttaatta atggtcagat taatcgctaa
    15601 cccgctgata ttttcggaaa cctcaatttg aggaagtcgg aagcgtcacc cttcatattc
    15661 tgaatggtca taatgaccac gcagaccatc aaggcaacca caacgaacat cgttacggtt
    15721 ggagctttta ccccactggg ggaaaggtat tacgataatc ataacaccta ccgaacaggt
    15781 aaaacccacc agcctgatta acagttaacc ggaaaaaaaa tccggtagcc ccgttatggg
    15841 gatccccata tcgacattaa cgcccctcat gaattggtgc gcttccccct ggaaagatta
    15901 cccgcctgta tatttcttgt gtctatttgt tccacgttgt ttcatacagt gcaccgaacg
    15961 gtgtagttaa tggtgtagtc aatttgctgt ttttagcact ttttgcagtg tgggttgttt
    16021 aattaaatca atgggttgcc ttgctgtggt gataaaagga catatttatt catctttcgg
    16081 cgtagaatgc tggcgaccaa aaatcacctc catccgcgca ccgccaagca tgctctctcc
    16141 ggcgacgatt ttaccctcat attgcttggt gatttcgcgg gctaccgcca gccccacacc
    16201 ttgcccaggg cgtaaagtat caacccgttg accacggtcg aaaatgacct ctcgcttgct
    16261 taatggaata ccggggccat catcctcgac cacaatatag agatgctcgt cggtttgcct
    16321 tgcagaaatt tcgacaaact cgaggcaata tttacaggca ttatccagta cattgcccat
    16381 cacctcgaca aaatcgttct gctcaccgac aaagctgatc tctggcgaaa tatcgagaga
    16441 gatattgacc cctttgcgtt gatacacttt gttcagcgcc gaggtgagat tgtccagcag
    16501 tggggcgacc ggatgcagct cgcggctgag caatgtcccg ccgtgcatac tggcacgatg
    16561 caggtagtag ccaatttgct gtgaaatgcg gctgatttgc tccagcatta ccggttcagc
    16621 atcactgacg ctcatctttt cactacgtag agaacgcagc gtactttgca gcaccgccag
    16681 tggcgttttc agactatggg tcaggtcggt gagcgttgta cgatatttgt cgtaacgttc
    16741 gcgttcactt tttaacaatc ggttcaggtt tcgtaccaga ctggtcagtt ctcgcgttgt
    16801 ggctggattg agcaattcgc ggttatgttc ttccagttcg cggacttctt ttgccagggc
    16861 ttcgatgggg cgtaaactcc accaggcggc gacccacagc agcgggatca ctaacagcag
    16921 attggctgag agcacataga taaaccagct ccagaccata taggaacttt ttagctccac
    16981 cggaatggta tccaccacca caatggttaa ctttggcatc cgcgatgttg ccgggtagac
    17041 gtttaccgcc accgagtggg tcatctccgc gtcgtcatca tcttcccgca cttcctgcaa
    17101 ctgttgctgt atcgaatgat ctccactcag caagaggctg gtatcgttaa catccgcttc
    17161 aatttcatga aaaccattcg atttcagcca gtcaggctgg atcatcttca tcagccaggg
    17221 cacgtcacgt tgcgcccata aaagctgccc gttctcatca taaattagcg tcatggtggg
    17281 gctttgcttg tcgatatttt cgggtaactc gacatgcaac ttattgtttt cccacttcgc
    17341 aagggtatag aacagattgc tctcgccacg taacagccga aacgtagttt tatcgaaact
    17401 gacgctataa ccgatcagcg cgaccattcc gtaggcaagc gaaagcacca gtactaccgc
    17461 tgccgttgcc aacagaaaac gtacccgcag cgagagcggg aaaaaaagac gcagtaattt
    17521 tttcatcagc gcaattcgaa caaatagccc tggccgcgaa cggtagtaat cacttcttgc
    17581 ggatattgtg cctgaatttt tttgcgcaga cgtcccatca gtacatcaat ggtatggctt
    17641 tcccgcagct ccgcatccgg atagagttgt agcattaacg aatctttgct gaccactttg
    17701 ccattattgc gtatcaacgt ttccataatg gtgtattcga acgcggtcag tttgatcact
    17761 tcgtcattaa tagataattc acggcgagag agatcaacct gaaacggggg gagcgaaatg
    17821 acctgtgaag ccaggccgct attacgccgc attaatgcct gcattcgcgc catcacctct
    17881 tcaatatgaa acggtttagt cacgtaatca tcagcaccgg cacttaatac ttcgactttg
    17941 tcctgccagc tttcacgggc ggttaatacc agaatcggca gtgaaacatc attgctacgc
    18001 cagcggcaaa tcagtgacag accgtcctcg tctggcaatc cgagatcgac aatcgcaata
    18061 tccggtaaat gttcattgag ataataatcg gcttctttgg catcttctgc atcatcgacc
    18121 tgatgaccag catcctgaat ctgaacttta aggtggtgac gtaacaacgc attgtcttca
    18181 acaaccagta cgcgcatttt tatttctccc tgtcttaatt attaaaatag tgtaacgcga
    18241 ttatgtggtt atgggggtaa acattaaata aaccagcggg ggaggggagg taaagtgaaa
    18301 aaataaaaag cggataatct taataagcag gccggacagc atcgccatcc ggcactggta
    18361 cgagtttatt tcagctcatc aaccatggtg atggcgcgac caatgtagtt agccggcgtc
    18421 atcgctttca ggcgggcttt ctcttcttct ggcaacgcca gaccgtcgat aaactgcttc
    18481 atgccttcgg cgtcaacgcg cttaccgcga gtcagctctt tcagcttctc gtacggtttt
    18541 tcgatgccat agcgacgcat aactgtctgg attggttctg ccagcacttc ccagttgtga
    18601 tccagctcat ccagcagatg gtcacggttc acttccagtt tgctcacgcc tttcagggtg
    18661 gattgatacg caatcagcgc ataaccgata cccacgccga ggttacgcag cacggtggag
    18721 tcggtcaggt cacgctgcca gcgggaaacc ggcagtttgc ttgccagatg ctgcaatacc
    18781 gcgttggaaa ggcccagatt cccttcggag ttttcgaagt cgatcgggtt aactttatgc
    18841 ggcatggtgg aagaaccaat ctcaccagca atggttttct gtttgaagtg gttaagggcg
    18901 atataacccc agacgtcacg gtcaaagtcg atcagaatgg tgttgaagcg cgcaacgcaa
    18961 tcaaacagtt cggcaatgta gtcgtgcggt tcaatctggg tagtgtacgg gttccattga
    19021 atacccagcg aggtgacgaa ctcttcgctg aactgatgcc agtcaacttc cgggtaagcg
    19081 gcgatgtggg cgttatagtt accgaccgcg ccgttgattt tgccgaggat ctccacctgg
    19141 ttaagctggc ggtactggcg ctccatacgg taggcgacgt ttgccatctc tttaccgatg
    19201 gttgacggcg tggctggctg accgtgggta cgggacagca gcgggatatc gcgatactga
    19261 acggcgagat ctttaatgcc atcaattagt tgacgccagt acggcaggat cacttcatca
    19321 cgcgcggttt tcagcattaa tgcgtgggag aggttattga tatcttccga agtacaggca
    19381 aagtggatga attcagaaac cgcgtgcagt tccgggatct ccgccacttt ttctttcagg
    19441 aaatactcaa ccgctttaac gtcgtggtta gtagtacgct cgatggtttt gatgcgtgcg
    19501 gcatcttctt cgctgaaatt ggcgacaatt gcatcaaggt aaccgattgc gtcggcagca
    19561 aaagcaggaa cttccttgat cgctgcgtgc gcggccagtt tttgcagcca acgtacttca
    19621 acttgtacac ggaatttcag caaaccatat tcgctgaaaa tcccgcgcag cgcgctgact
    19681 ttatcgccgt agcgtccatc gacaggggaa acggcggtca gtgaggataa ttccatagat
    19741 cacaactccg gggttaaatg agcaagaatt tgttttgcct gagtggtcag gcgattacga
    19801 gaaaacatca gttgcagacg tccgccgccg acctggtgcc agagcacggc ggcgcgaatg
    19861 cctgccagca gggttgcgcg aactttcgcc tgcacttgtg ggctttgcag tacagcaggg
    19921 gaaccggtga cctgaatgcg cgggccaagc gggctgatca catcaacata gatagcagcc
    19981 atcgcgctca tcagcgtttc ggactgtaaa tcgaagtgtt cgagctggcg ttgcaggccg
    20041 ttgatccggt tgcccagagt gtcgagcgca cctttcgctg aggagagttt gcgcttaagc
    20101 accatcaggc tgagtgtgta gcgggttaat tcggcgttta agccctggcg actgctggca
    20161 ttgagcacgc cgagcagggt ttccagcccg acgcgcaggt tggcttcgct accgccaaaa
    20221 accgccagcg tcgagctggg gttcatatca ataatactgt tgagtgagac gtgtagcgca
    20281 tcggcatcac aatgcccctg gtgagcgagt tgttgcacca ggcgtgccga ctgacaaata
    20341 ccggccaggg cgagggtgat gtcatagtaa ttctttgcca cgttcactgc ttccttgttt
    20401 taagtaaaga taataatcag atcggcagcg gcagacgctg ctcaataata ccgccaccga
    20461 ggcacacttc accgttatag aagacggcag actggcccgg cgtcacggcg gcaacgggct
    20521 catcgaaaat cacttcaatg cgatcatcgt ccagcgcctt gacggtgcag gggatatcgg
    20581 tctggcgata gcgggttttt accgtgcaac gcatagtacc ggtgaatggt tcacgatcga
    20641 cccagtgcag ctgctgggca atcagaccga cagacatcag acgcgggtgt tcatggccct
    20701 gcgcaacaat cagaatgttg ttttcgacgt ctttatcgac cacataccac ggttcttcgg
    20761 tgccttcttt ggtgccaccg atacccagac ctttgcgctg accgagggtg tgatacatca
    20821 gcccctgatg ctcaccaatt tcatcgccat cgactgtaat gattttgccc ggttgcgccg
    20881 ggagataacg gccaaggaac tcgcggaatt tacgttcgcc aatgaagcaa atgccggtag
    20941 agtctttttt cttcgcggtg accagaccaa gatcttcagc aatcttacgc acctgcggtt
    21001 tttccagttc gccgaccggg aacaggcttt gcgcaatctg ctcatggctg agcgtataaa
    21061 ggaagtagct ctggtcttta ttgctgtcca gaccacgcag caggcggctc ttgccatcga
    21121 catctgcccg acgaacgtaa tgaccggtag cgatataatc ggcacctaaa tcttcggcgg
    21181 caaattcgag gaaggcttta aatttgatct ctttgttgca cagaatatcc ggattcggcg
    21241 tgcgaccggc tttatattcg gcaaggaaca gttcgaagac gttgtcccag tactcggcag
    21301 caaagttaac ggtgtgcagt tcaatgccga gcttgtcgca gacagcctgg gcatcagcca
    21361 gatccgctgc cgctgtgcaa tattcctcac cgtcgtcttc ttcccagttc ttcataaaca
    21421 ggccttcgac ctgatatccc tgttgttgca acagccaggc agaaacggag gaatcgacac
    21481 cgccggacat gccgacgatt actttttttg cggtttcaga cattggatca ctcacgacat
    21541 tgaacttcaa ggcggcgtat tctatcacgc agccttttag atgacaccct ttgtaaaagg
    21601 ccagttaaaa tcgccaatca tctccagcgg atagcgttgc ccgctttgat aacaacggat
    21661 actttccgcc accagcggcg aacgaagatt tgacgcctgt aaaatttctt cggcgctgac
    21721 ccaacgacag cagtcgatat cgctgtcatg aggctgggtc gggcatattt gctcaagctc
    21781 aatggcaaag aggaaacgca aaaacggcgt tttatctggc gcaatccact gatgcatacg
    21841 aataaagtgt tgcggctgcg cgctgatgcc ggtttcttcc cacagctcac gggcggctgc
    21901 ttccactaag gtttcatcgg cttccagatg cccggcaggt tggttccata aggctttacc
    21961 attaatcgtc tcttcaacga ctaaaaattt cccctctgcg tgcactacgc aagcaacggt
    22021 aacgtgcggt ttaaacattg tgcatcctta ttctgttgct tctcgccatt cgccattggc
    22081 aaggttatcc aaagagtaat cacccatcgc atagcgaatc agtcgcagcg tggggaaacc
    22141 aacatgggcg gtcatgcggc gcacctggcg attacgtcct tcatataagg tgatcttcag
    22201 ccagctggtg ggaatacttt tgcgttcacg aattggtgga ttccgcggcc ataaccacgc
    22261 gggttcgtca accagctccg cgcctgcggg cagggtaggg ccatcattta aggttacgcc
    22321 attgcgcaag gcttcaagtg cgtcttgtgt gggaatacct tccacctgca cataatagat
    22381 ttttccggtg cgtttgcccg gctgggttaa acgcgcctgc aacgcgccgt tattggtcag
    22441 caccagtaac ccttcgctat cgcggtcaag acgacctgct gcataaacgc cctgaaccgg
    22501 gatgaattct tttaatgttt tgcgtccggc ttcatcggtg aactgcggaa gaacatcgta
    22561 gggtttattg aacaggatca cacgcgtggg ctggttttca ggtttacgcc tggtagaacg
    22621 ttgtgagctg aatcgcttaa cctggtgatt tctaaaagaa gttttttgca tggtattttc
    22681 agagattatg aattgccgca ttatagccta ataacgcgca tctttcatga cggcaaacaa
    22741 tagggtagta ttgacaagcc aattacaaat cattaacaaa aaattgctct aaagcatccg
    22801 tatcgcagga cgcaaacgca tatgcaacgt ggtggcagac gagcaaacca gtagcgctcg
    22861 aaggagaggt gaatggaaag taaagtagtt gttccggcac aaggcaagaa gatcaccctg
    22921 caaaacggca aactcaacgt tcctgaaaat ccgattatcc cttacattga aggtgatgga
    22981 atcggtgtag atgtaacccc agccatgctg aaagtggtcg acgctgcagt cgagaaagcc
    23041 tataaaggcg agcgtaaaat ctcctggatg gaaatttaca ccggtgaaaa atccacacag
    23101 gtttatggtc aggacgtctg gctgcctgct gaaacccttg atctgattcg tgaatatcgc
    23161 gttgccatta aaggcccgct gaccactccg gttggtggcg gtattcgctc tctgaacgtt
    23221 gccctgcgcc aggaactgga tctctacatc tgcctgcgtc cggtacgtta ctatcagggc
    23281 actccaagcc cggttaaaca ccctgaactg accgatatgg ttatcttccg tgaaaactcg
    23341 gaagacattt atgcgggtat cgaatggaaa gctgactctg ccgacgccga gaaagtgatt
    23401 aaattcctgc gtgaagagat gggcgtgaag aaaattcgct tcccggaaca ttgcggtatc
    23461 ggtattaagc cgtgttctga agaaggcacc aaacgtctgg ttcgtgcagc gatcgaatac
    23521 gcaattgcta acgatcgtga ctctgtgact ctggtgcaca aaggcaacat catgaagttc
    23581 accgaaggcg cgtttaaaga ctggggctac gagctggcgc gtgaagagtt tggcggtgaa
    23641 ctgatcgacg gcggcccgtg gctgaaagtt aaaaacccga acaccggcaa agagatcgtc
    23701 attaaagacg tgattgctga tgcattcctg caacagatcc tgctgcgtcc ggctgaatat
    23761 gatgttatcg cctgtatgaa cctgaacggt gactacattt ctgacgccct ggcagcgcag
    23821 gttggcggta tcggtatcgc ccctggcgca aacatcggtg acgaatgcgc cctgtttgaa
    23881 gccacccacg gtactgcgcc gaaatatgcc ggtcaggaca aagttaaccc aggttctatt
    23941 attctctctg ctgagatgat gttacgccat atgggttgga ctgaagcggc tgacctgatt
    24001 gttaaaggta tggaaggcgc aatcaatgcc aagaccgtaa cctatgactt cgaacgtctg
    24061 atggaaggcg ctaaactgct gaaatgttca gagtttggtg acgcgatcat caagaatatg
    24121 taatcactac atgtgttaaa tattgaaacg ggcgtataac acgcccgttg ttttatgatg
    24181 atttaaaatc ttccccaaaa ctttccccaa aacccttccc caaaactggc tattttctat
    24241 gctgttttga tatctacgat aatccagtct ttaccacgat catcattgta tcggtcggtc
    24301 atttttctgg atttatgacc taacaacttt tgcgtatcca gaccctgttc ccgatataac
    24361 cgttctgaca gagatcgctg ctcatgaaat gtgggcgcag ttccttgctc ccattttatg
    24421 ccacattttt ccctggcctt tttaaaagcc gttgtaagag tatttgcaga cacctggtct
    24481 cctctgtttg cttgagaggt agtgtgacgg taatggacca gatatttact aacaacagca
    24541 tccctgcact gagatataac ttcacgaagg gtaatattca gagcatcgca tttcaggtta
    24601 agcggaatag caagttttga accggttttt tcctgagtaa tgtgcaacat gtcgtcccag
    24661 atatcagaga atttcaaatt gcagatatcg cttaaacgtt gtccagtgac aagagcaagt
    24721 agcatcccgc attttaaata gggctgccgt ctgcttacgc tgtcaaatat tgcctgccat
    24781 tcgggcagtg ataatctttg acggtttact cgatttcgcg gttgttttgt tgcctgcgct
    24841 gggttaaatc ctggcggaac atgtcctgcg tgttgtgctt ctttgaagac gtcgatcaac
    24901 accattctca cgacttgcgc catcctgtta tgaccttcag cctttacagc atcaattatt
    24961 tcggcaatat caagtgcggt aatatccttg aggtgttgca ttccacaatg ctcacggaaa
    25021 agacggatgg gtttgccttt ttgccgatag gagttgggtc ttagttcatt atgttgcagc
    25081 ctgtcctcct ggatagaatt atatttatca agccattctg tcaccgtaat gtctgagcgc
    25141 ctgcctttca ttctttccag acgctcattg acgcttaata tttgtcgggt acgttgttca
    25201 gcaataatgg tatttgcttc agtagcaact tgttttgctt cattctcatc agttcctaag
    25261 ctatgaaaac gaccggatag tggatgtttg tattgccaat atacctttcc ggttcgctta
    25321 tctaatttgc aatataaatt gggtatagag attttgtgag atcgggggct agcagccatc
    25381 agcgattatc cgttggagtt ttgggtttgc gtttattggg agttgcggtt ctgcaagcgt
    25441 tcctacaaaa cgggaatttc ggtcaatcat ccagtagcga ccaactttta tagcgggtgg
    25501 ggccatcatt ttcccttgcg cgtatttttt cagaactcgc tcacttggtg ctaagtcccc
    25561 aaattcttct ttagcccagt cccgtaaagt gattagtcga gacatttgtc ctcctcttag
    25621 ctgctgaggg agtttgtgac cgatatatct gacatgatat taagctcatg gcaggtacat
    25681 ctcttgactg gtcatagaga taaatttaat gctgagaaat gcagtattga atttatcaat
    25741 ttttcaccag cttctggtct ttctggctgt catgcgcaaa acatatggct ttaataaaaa
    25801 actggattgg gttagcaacg agcaactttc cgaattaacc gggatattgc cgcacaagtg
    25861 ttctgctgca aaaagtgttc tggtaaagcg tgggattttt attcagagcg ggcggaatac
    25921 cggcattaat aatgtggtca gtgaatggtc aacattaccc gaatcaggta agaaaaataa
    25981 agtttacctg aaagaggtaa atttacctga atcaggtaaa aaaagtttac ccaaatcagg
    26041 taaaggcgtt tacccgaatc aggtaaacac aaaagacaaa ctaacaaaag acaatataaa
    26101 acctttttcg tccgagaatt ctggcgaatc ctctgaccaa ccagaaaacg atcttcctgt
    26161 ggtgaaaccg gatgctgcaa ttcagagcgg cagcaagtgg gggacagcag aagacctgac
    26221 cgccgcagag tggatgtttg acatggtgaa gaccatcgcg ccatcagcca gaaaaccgaa
    26281 ttttgcaggg tgggctaacg atatccgcct gatgcgtgaa cgtgacggac gtaaccaccg
    26341 cgacatgtgc gtgctgttcc gctgggcatg ccaggacaac ttctggtccg gtaacgtgct
    26401 aagtccggcc aaactccgcg acaagtggac ccagctcgaa atcaaccgta acaagcaaca
    26461 ggcaggcatg atagccagca aaccaaaact cgacctgaca aacactgact ggatttacgg
    26521 ggtggattta tgaaaaacat cgccgcacag atggttaact ttgaccgtga gcagatgcgt
    26581 cggatcgcca acaacatgcc ggaacagtac gacgaaaagc cgcaggtaca gcaggtagcg
    26641 cagatcatca acggtgtgtt cagccagtta ctggcaactt tcccggcgag tctggctaac
    26701 cgtgaccaga acgaactgaa cgaaatccgc cgccagtggg ttctggcttt ccgggaaaac
    26761 gggatcacca caatggaaca ggttaacgca ggaatgcgcg tagcccgtcg gcagaatcga
    26821 ccatttctgc catcacccgg gcagtttgtt gcatggtgcc gggaagaagc atccgttatc
    26881 gccggactgc caaacgtcag cgagctggtt gatatggttt acgagtattg cc
//
LOCUS       DBFIFI010000049        25542 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.049, whole genome shotgun sequence.
ACCESSION   DBFIFI010000049 DBFIFI010000000
VERSION     DBFIFI010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25542
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.049"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..415
                     /gene="bfr"
                     /locus_tag="N7B39_001926"
     CDS             <1..415
                     /gene="bfr"
                     /locus_tag="N7B39_001926"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HGU2421917.1"
                     /translation="INQYFLHARMFKNWGLMRLNDVEYHESIDEMKHADKYIERILFL
                     EGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLREAIAYADSVHDYVSRDMMIEI
                     LADEEGHIDWLETELDLIGKIGLQNYLQSQIKVND"
     gene            complement(412..879)
                     /locus_tag="N7B39_001927"
     CDS             complement(412..879)
                     /locus_tag="N7B39_001927"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HGU2421918.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGAIIGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR"
     gene            1258..1569
                     /gene="rpsJ"
                     /locus_tag="N7B39_001928"
     CDS             1258..1569
                     /gene="rpsJ"
                     /locus_tag="N7B39_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HGU2421919.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            1602..2231
                     /gene="rplC"
                     /locus_tag="N7B39_001929"
     CDS             1602..2231
                     /gene="rplC"
                     /locus_tag="N7B39_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HGU2421920.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2242..2847
                     /gene="rplD"
                     /locus_tag="N7B39_001930"
     CDS             2242..2847
                     /gene="rplD"
                     /locus_tag="N7B39_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HGU2421921.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            2844..3146
                     /gene="rplW"
                     /locus_tag="N7B39_001931"
     CDS             2844..3146
                     /gene="rplW"
                     /locus_tag="N7B39_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HGU2421922.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3164..3985
                     /gene="rplB"
                     /locus_tag="N7B39_001932"
     CDS             3164..3985
                     /gene="rplB"
                     /locus_tag="N7B39_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HGU2421923.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4002..4280
                     /gene="rpsS"
                     /locus_tag="N7B39_001933"
     CDS             4002..4280
                     /gene="rpsS"
                     /locus_tag="N7B39_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HGU2421924.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4295..4627
                     /gene="rplV"
                     /locus_tag="N7B39_001934"
     CDS             4295..4627
                     /gene="rplV"
                     /locus_tag="N7B39_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HGU2421925.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            4645..5346
                     /gene="rpsC"
                     /locus_tag="N7B39_001935"
     CDS             4645..5346
                     /gene="rpsC"
                     /locus_tag="N7B39_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HGU2421926.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5359..5769
                     /gene="rplP"
                     /locus_tag="N7B39_001936"
     CDS             5359..5769
                     /gene="rplP"
                     /locus_tag="N7B39_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HGU2421927.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            5769..5960
                     /gene="rpmC"
                     /locus_tag="N7B39_001937"
     CDS             5769..5960
                     /gene="rpmC"
                     /locus_tag="N7B39_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HGU2421928.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            5960..6214
                     /gene="rpsQ"
                     /locus_tag="N7B39_001938"
     CDS             5960..6214
                     /gene="rpsQ"
                     /locus_tag="N7B39_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HGU2421929.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6379..6750
                     /gene="rplN"
                     /locus_tag="N7B39_001939"
     CDS             6379..6750
                     /gene="rplN"
                     /locus_tag="N7B39_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HGU2421930.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            6761..7075
                     /gene="rplX"
                     /locus_tag="N7B39_001940"
     CDS             6761..7075
                     /gene="rplX"
                     /locus_tag="N7B39_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HGU2421931.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7090..7629
                     /gene="rplE"
                     /locus_tag="N7B39_001941"
     CDS             7090..7629
                     /gene="rplE"
                     /locus_tag="N7B39_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HGU2421932.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            7644..7949
                     /gene="rpsN"
                     /locus_tag="N7B39_001942"
     CDS             7644..7949
                     /gene="rpsN"
                     /locus_tag="N7B39_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HGU2421933.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            7983..8375
                     /gene="rpsH"
                     /locus_tag="N7B39_001943"
     CDS             7983..8375
                     /gene="rpsH"
                     /locus_tag="N7B39_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HGU2421934.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8388..8921
                     /gene="rplF"
                     /locus_tag="N7B39_001944"
     CDS             8388..8921
                     /gene="rplF"
                     /locus_tag="N7B39_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HGU2421935.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            8931..9284
                     /gene="rplR"
                     /locus_tag="N7B39_001945"
     CDS             8931..9284
                     /gene="rplR"
                     /locus_tag="N7B39_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HGU2421936.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9299..9802
                     /gene="rpsE"
                     /locus_tag="N7B39_001946"
     CDS             9299..9802
                     /gene="rpsE"
                     /locus_tag="N7B39_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HGU2421937.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            9806..9985
                     /gene="rpmD"
                     /locus_tag="N7B39_001947"
     CDS             9806..9985
                     /gene="rpmD"
                     /locus_tag="N7B39_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HGU2421938.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            9989..10423
                     /gene="rplO"
                     /locus_tag="N7B39_001948"
     CDS             9989..10423
                     /gene="rplO"
                     /locus_tag="N7B39_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HGU2421939.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10431..11762
                     /gene="secY"
                     /locus_tag="N7B39_001949"
     CDS             10431..11762
                     /gene="secY"
                     /locus_tag="N7B39_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HGU2421940.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            11794..11910
                     /gene="rpmJ"
                     /locus_tag="N7B39_001950"
     CDS             11794..11910
                     /gene="rpmJ"
                     /locus_tag="N7B39_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HGU2421941.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12057..12413
                     /gene="rpsM"
                     /locus_tag="N7B39_001951"
     CDS             12057..12413
                     /gene="rpsM"
                     /locus_tag="N7B39_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HGU2421942.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12430..12819
                     /gene="rpsK"
                     /locus_tag="N7B39_001952"
     CDS             12430..12819
                     /gene="rpsK"
                     /locus_tag="N7B39_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HGU2421943.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            12853..13473
                     /gene="rpsD"
                     /locus_tag="N7B39_001953"
     CDS             12853..13473
                     /gene="rpsD"
                     /locus_tag="N7B39_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HGU2421944.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            13499..14488
                     /gene="rpoA"
                     /locus_tag="N7B39_001954"
     CDS             13499..14488
                     /gene="rpoA"
                     /locus_tag="N7B39_001954"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HGU2421945.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            14529..14912
                     /gene="rplQ"
                     /locus_tag="N7B39_001955"
     CDS             14529..14912
                     /gene="rplQ"
                     /locus_tag="N7B39_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HGU2421946.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15019..15387
                     /locus_tag="N7B39_001956"
     CDS             15019..15387
                     /locus_tag="N7B39_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HGU2421947.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLNKINDN"
     gene            15398..15823
                     /gene="zntR"
                     /locus_tag="N7B39_001957"
     CDS             15398..15823
                     /gene="zntR"
                     /locus_tag="N7B39_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HGU2421948.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            15879..16097
                     /gene="arfA"
                     /locus_tag="N7B39_001958"
     CDS             15879..16097
                     /gene="arfA"
                     /locus_tag="N7B39_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HGU2421949.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(16094..16504)
                     /gene="mscL"
                     /locus_tag="N7B39_001959"
     CDS             complement(16094..16504)
                     /gene="mscL"
                     /locus_tag="N7B39_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HGU2421950.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(16634..18010)
                     /gene="trkA"
                     /locus_tag="N7B39_001960"
     CDS             complement(16634..18010)
                     /gene="trkA"
                     /locus_tag="N7B39_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HGU2421951.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18032..19321)
                     /gene="rsmB"
                     /locus_tag="N7B39_001961"
     CDS             complement(18032..19321)
                     /gene="rsmB"
                     /locus_tag="N7B39_001961"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HGU2421952.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKSMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNTSDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRINRTHHSRDTWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(19367..20314)
                     /gene="fmt"
                     /locus_tag="N7B39_001962"
     CDS             complement(19367..20314)
                     /gene="fmt"
                     /locus_tag="N7B39_001962"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HGU2421953.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWVEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(20329..20838)
                     /gene="def"
                     /locus_tag="N7B39_001963"
     CDS             complement(20329..20838)
                     /gene="def"
                     /locus_tag="N7B39_001963"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HGU2421954.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            20968..22092
                     /gene="dprA"
                     /locus_tag="N7B39_001964"
     CDS             20968..22092
                     /gene="dprA"
                     /locus_tag="N7B39_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HGU2421955.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLARQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLAKHGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDEVTPVDVVAERAGRP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22064..22537
                     /gene="smg"
                     /locus_tag="N7B39_001965"
     CDS             22064..22537
                     /gene="smg"
                     /locus_tag="N7B39_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HGU2421956.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            22566..23108
                     /locus_tag="N7B39_001966"
     CDS             22566..23108
                     /locus_tag="N7B39_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HGU2421957.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCINYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23113..23685
                     /gene="tsaC"
                     /locus_tag="N7B39_001967"
     CDS             23113..23685
                     /gene="tsaC"
                     /locus_tag="N7B39_001967"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HGU2421958.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            23690..24508
                     /gene="aroE"
                     /locus_tag="N7B39_001968"
     CDS             23690..24508
                     /gene="aroE"
                     /locus_tag="N7B39_001968"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HGU2421959.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFV
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKPTADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            24505..24762
                     /locus_tag="N7B39_001969"
     CDS             24505..24762
                     /locus_tag="N7B39_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HGU2421960.1"
                     /translation="MNQAIQFPDREEWVENKKCVCFPALVNGMQLTCAISGDSLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(24738..25292)
                     /locus_tag="N7B39_001970"
     CDS             complement(24738..25292)
                     /locus_tag="N7B39_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HGU2421961.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6538 a         6077 c         6645 g         6282 t
ORIGIN      
        1 aatcaatcag tattttctcc atgcccggat gtttaaaaac tggggactga tgcgtcttaa
       61 tgacgtcgaa taccatgaat ccatcgatga gatgaaacac gctgataaat atatcgagcg
      121 tattttgttt ttagaaggaa tccccaactt acaggattta ggtaagctag ggatcggtga
      181 agatgtcgaa gagatgctgc aatccgacct gcgattagag ctggaaggtg cgaaggatct
      241 gcgtgaggct atcgcgtatg ccgacagcgt tcatgactat gttagccgcg atatgatgat
      301 cgagatcctt gccgatgaag aaggtcatat cgactggctc gaaactgaac tggatctcat
      361 cggtaaaatc ggcctacaaa attacctgca atcacaaatt aaagttaatg attaacggcc
      421 tatcagcaaa ctatcccagc ctataacgaa acccgcagcc gccagaaatg gcccaaaagg
      481 cagcgggttt tttaatgatt cttttcctct catgagcaga ccaataacta cggctccgca
      541 agcgaatgag gcagcaagga aaaccagtcg tggtaagaag gcccatgaat gccaggcgcc
      601 aagggcggcg agaaacttca catcaccata gcctaatcct tctttatggc gcagtatgcg
      661 atacccccag taaatgacag cgaatgtgcc gtaaccaata attgcccccc ataacgcatc
      721 ggctaaacca tcaggatgac aaacttgata gaacaaaaga cctgaccaga gtaacgggca
      781 cgtaaatctg tcaggtaaca agccatgctt tgcatcccag aaaaaaagta gggctgataa
      841 acaagcgtac aaaattagaa agggaagggt agtgtccata aaacatcctc tgtaattgga
      901 gtatcaccat cgaataactc cggatgctca gcaacagaga gagcgctgat ttgttaacct
      961 gattccggtg aaaaacgcta tcttcacaac cttacaaatt tcgtggaatg cagctcgcaa
     1021 tctgcgcgtt gcgattaatt tctgcccgat gcttgcgtca ggctcagatt tgcgtataat
     1081 gcgcgggctt gtcgtagttg acagcaggtt caatctgaac ctcagttagc cgaatttggc
     1141 tacctaacaa tgctcccaat cggggagcta cgtaagaacg gttacactct cccatcaatc
     1201 gtaatgggtc tgaggagtaa tcattttcgt ttataaaata attggagctc tggtctcatg
     1261 cagaaccaaa gaatccgtat ccgcctgaaa gcgtttgatc atcgtctgat cgatcaagca
     1321 accgcggaaa tcgtcgagac tgccaagcgc actggtgcgc aggtccgtgg tccgatcccg
     1381 ctgccgacac gcaaagagcg cttcactgtt ctgatctccc cgcacgtcaa caaagacgcg
     1441 cgcgatcagt acgaaatccg tactcacttg cgtctggttg acatcgttga gccaaccgag
     1501 aaaaccgttg atgctctgat gcgtctggat ctggctgccg gtgtagacgt gcagatcagc
     1561 ctgggttaat caggtcattg agcgattgag aggttgaaac aatgattggt ttagtcggta
     1621 aaaaagtggg tatgacccgt atcttcacag aagacggcgt ttctatccca gtaaccgtaa
     1681 tcgaagttga agcaaaccgc gttactcagg ttaaagacct ggctaacgat ggctaccgtg
     1741 ctattcaggt gaccaccggt gctaaaaaag ctaaccgtgt gaccaagcct gaagctggcc
     1801 acttcgctaa agctggcgta gaagctggcc gtggtctgtg ggaattccgc ctggctgaag
     1861 gcgaagagtt cactgtaggt cagagcatta gcgttgaact gtttgctgac gttaaaaaag
     1921 ttgacgtaac tggcacctct aaaggtaaag gtttcgcagg taccgttaag cgctggaact
     1981 tccgtaccca agacgctact cacggtaact ccttgtctca ccgcgttccg ggttctatcg
     2041 gtcagaacca gactccgggc aaagtgttca aaggcaagaa aatggcaggt cagatgggta
     2101 acgaacgtgt aaccgttcag agccttgacg tagtacgcgt tgacgctgag cgcaacctgc
     2161 tgctggttaa aggtgctgtc ccgggtgcaa ccggtagcga cctgatcgtt aaaccagctg
     2221 tgaaggcgta aggagatagc aatggaatta gtattgaaag acgcgcagag cgcgctgact
     2281 gtttccgaaa ctaccttcgg tcgtgatttc aacgaagcgc tggttcacca ggttgttgtt
     2341 gcttatgcag ctggtgctcg tcagggtact cgtgctcaga agactcgtgc tgaagtaact
     2401 ggttccggta aaaaaccgtg gcgccagaaa ggcaccggcc gtgcgcgttc tggttctatc
     2461 aagagcccga tctggcgttc tggtggcgtg acctttgctg ctcgtccgca ggaccacagt
     2521 caaaaagtta acaagaagat gtaccgcggc gcgctgaaaa gcatcctgtc cgaactggta
     2581 cgtcaggatc gtctgatcgt tgtcgagaag ttctctgtag aagcgccgaa aactaagctg
     2641 ctggcacaga aactgaaaga catggctctg gaagatgtgc tgatcatcac cggtgagctg
     2701 gacgaaaacc tgttcctggc tgcgcgcaac ctgcacaagg ttgacgtacg cgatgcaact
     2761 ggtatcgacc cggttagcct gatcgccttc gacaaagtcg taatgactgc tgatgctgtt
     2821 aagcaagttg aggagatgct ggcatgattc gtgaagaacg tctgctgaag gtgctgcgtg
     2881 caccgcacgt ttctgaaaaa gcgtctactg cgatggaaaa atccaacacc atcgtactca
     2941 aagttgctaa agacgcgacc aaagcagaaa tcaaagctgc tgtgcagaaa ctgtttgaag
     3001 tcgaagtcga agtcgttaac accctggtag ttaaagggaa agttaaacgt cacggacagc
     3061 gtatcggtcg tcgtagcgac tggaaaaaag cttacgtcac cctgaaagaa ggccagaatc
     3121 tggacttcgt tggcggcgct gagtaagtcg gaggagtaat acaatggcag ttgttaaatg
     3181 taaaccgaca tctccgggtc gtcgccacgt agttaaagtg gttaaccctg agctgcacaa
     3241 gggcaaacct tttgctccgt tgctggaaaa aaacagcaaa tccggtggtc gtaacaacaa
     3301 tggccgtatc accactcgtc atatcggtgg tggccacaag caggcttacc gtattgttga
     3361 cttcaaacgc aacaaagacg gtatcccggc agttgttgaa cgtcttgagt acgatccgaa
     3421 ccgttccgcg aacatcgcgc tggttctgta caaagacggt gaacgccgtt acatcctggc
     3481 ccctaaaggc ctgaaagctg gcgaccagat tcagtctggc gttgatgctg caatcaaacc
     3541 aggcaacacc ctgccgatgc gcaacatccc ggttggttct actgttcata acgtagaaat
     3601 gaaaccaggt aaaggcggtc agctggcacg ttccgctggt acttacgttc agatcgttgc
     3661 tcgcgacggt gcttatgtca ccctgcgtct gcgttctggt gaaatgcgta aagtcgaagc
     3721 agactgccgt gcaactctgg gcgaagttgg caatgctgag catatgctgc gcgttctggg
     3781 taaagcaggt gctgcacgct ggcgtggtgt tcgtccgacc gttcgcggta ccgcgatgaa
     3841 cccggtagac cacccacatg gtggtggtga aggtcgtaac tttggtaagc acccggtaac
     3901 tccgtggggc gttcagacca aaggtaagaa gacccgcagc aacaagcgta ctgataaatt
     3961 catcgtacgt cgccgtagca aataatttta gaggataagc catgccacgt tctctcaaga
     4021 aaggtccttt tattgacctg cacttgctga agaaggtaga gaaagcggtg gaaagcggag
     4081 acaagaagcc cctgcgcact tggtcccgtc gttcaacgat ctttcctaac atgatcggtt
     4141 tgaccatcgc tgtccataat ggtcgtcagc acgttccggt atttgtaacc gacgaaatgg
     4201 tcggtcacaa actgggtgaa ttcgcaccga ctcgtactta tcgcggccac gctgctgata
     4261 aaaaagcgaa gaagaaataa ggtaggagga agagatggaa actatcgcta aacatcgcca
     4321 tgctcgttct tctgctcaga aggttcgcct tgttgctgac ctgattcgcg gtaagaaagt
     4381 gtcgcaggct ctggatattt tgacctacac caacaagaaa gcggctgtac tggtcaagaa
     4441 agttctggaa tctgccattg ctaacgctga acacaacgat ggcgctgaca ttgacgatct
     4501 gaaagttacg aaaattttcg tagacgaagg cccgagcatg aagcgcatta tgccgcgtgc
     4561 aaaaggtcgt gcagatcgca tcctgaagcg caccagccac atcactgtgg ttgtgtccga
     4621 tcgctgagac tctggagact agcaatgggt cagaaagtac atcctaatgg tattcgcctg
     4681 ggtattgtaa aaccatggaa ctctacctgg tttgcgaaca ccaaagaatt cgctgacaac
     4741 ctggacagcg attttaaagt acgtcagtac ctgactaagg aactggctaa agcgtccgta
     4801 tctcgtatcg ttatcgagcg tccggctaag agcatccgtg taaccattca cactgctcgc
     4861 ccgggtatcg ttatcggtaa aaaaggtgaa gacgtagaaa aactgcgtaa ggtcgtagcg
     4921 gacatcgctg gcgttcctgc acagatcaac atcgccgaag ttcgtaagcc tgaactggac
     4981 gcaaaactgg ttgctgacag catcacttct cagctggaac gtcgcgttat gttccgtcgt
     5041 gctatgaagc gtgctgtaca gaacgcaatg cgtctgggcg ctaaaggtat taaagttgaa
     5101 gttagcggcc gtctgggcgg cgcggaaatc gcacgtaccg aatggtaccg cgaaggtcgc
     5161 gtaccgctgc acactctgcg tgctgacatc gactacaaca cctctgaagc gcacaccact
     5221 tacggtgtaa tcggcgttaa agtgtggatc ttcaaaggcg agatcctggg tggtatggct
     5281 gctgttgaac aaccggaaaa accggctgct cagcctaaaa agcagcagcg taaaggccgt
     5341 aaataaggag cgtcgctgat gttacaacca aagcgtacaa aattccgtaa aatgcataaa
     5401 ggccgtaacc gcggtctggc gcagggtacg gatgttagct tcggcagctt cggtctgaaa
     5461 gctgttggcc gtggtcgtct gactgcccgt cagatcgaag cagcacgtcg tgctatgacc
     5521 cgtgcagtta agcgtcaagg taagatctgg atccgtgtgt tcccggacaa accgatcact
     5581 gaaaagccgc tggcagtgcg tatgggtaaa ggtaaaggta acgtggagta ttgggttgcc
     5641 ttgattcagc cgggtaaagt cctgtatgaa atggacggtg ttccggaaga gctggcccgt
     5701 gaagcattca agctggcagc agcgaaactg ccgattaaaa ccacctttgt aactaagacg
     5761 gtgatgtaat gaaagcaaaa gagctgcgtg agaagagcgt tgaagagctg aacaccgagc
     5821 tgctgaacct gctgcgtgag cagttcaacc tgcgtatgca ggctgcaagt ggccagctgc
     5881 aacagtctca cctgttgaag caagtgcgtc gcgatgtcgc acgcgttaag actttactga
     5941 acgagaaggc gggtgcgtaa tgaccgataa aatccgtact ctgcaaggtc gcgttgttag
     6001 cgacaaaatg gagaaatcca ttgttgttgc tatcgaacgt tttgtgaaac acccgatcta
     6061 cggtaaattc atcaagcgta cgaccaaact gcacgtacat gacgagaaca acgaatgcgg
     6121 tatcggtgac gtggttgaaa tccgcgaatg ccgtccgctg tccaagacta aatcctggac
     6181 gctggttcgc gttgtagaga aagcggttct gtaatacagt acactctctc gatacgaata
     6241 aacggctcag aaatgagccg tttatttttt ctacccatat ccttgaagcg gtgttataat
     6301 gccgcgccct cgatatgggg atttttaacg acctgatttt cgggtctcag tagtagttga
     6361 cattagcgga gcactaaaat gatccaagaa cagactatgc tgaacgtcgc cgacaactcc
     6421 ggtgcacgtc gcgtaatgtg tatcaaggtt ctgggtggct cgcaccgtcg ctacgcaggc
     6481 gtaggcgaca tcatcaagat caccatcaaa gaagcaattc cgcgtggtaa ggtcaaaaaa
     6541 ggtgatgtgc tgaaggcggt agtggtgcgc accaagaagg gtgttcgtcg cccggacggt
     6601 tctgtcattc gcttcgatgg taatgcttgt gttcttctga acaacaacag cgagcagcct
     6661 atcggtacgc gtatttttgg gccggtaact cgtgagcttc gtagtgagaa gttcatgaaa
     6721 attatctctc tggcaccaga agtactctaa ggagcgaatc atggcagcga aaatccgtcg
     6781 tgatgacgaa gttatcgtgt taaccggtaa agataaaggt aaacgcggta aagttaagaa
     6841 tgtcctgtct tccggcaagg tcattgttga aggtatcaac ctggttaaga aacatcagaa
     6901 gccggttccg gccctgaacc aaccgggtgg catcgttgaa aaagaagccg ctattcaggt
     6961 ttccaacgta gcaatcttca atgcggcaac cggcaaggct gaccgtgtag gctttagatt
     7021 cgaagacggc aaaaaagtcc gtttcttcaa gtctaacagc gaaactatca agtaatttgg
     7081 agtagtacga tggcgaaact gcatgattac tacaaagacg aagtagttaa aaaactcatg
     7141 actgagttta actacaattc tgtcatgcaa gtccctcggg tcgagaagat caccctgaac
     7201 atgggtgttg gtgaagcgat cgctgacaaa aaactgctgg ataacgcagc agcagacctg
     7261 gcagcaatct ccggtcaaaa accgctgatc accaaagcac gcaaatctgt tgcaggcttc
     7321 aaaatccgtc agggctatcc gatcggctgt aaagtaactc tgcgtggcga acgcatgtgg
     7381 gagttctttg agcgcctgat cactattgct gtacctcgta tccgtgactt ccgtggcctg
     7441 tccgctaagt ctttcgacgg tcgtggtaac tacagcatgg gtgtccgtga gcagatcatc
     7501 ttcccagaaa tcgactacga taaagtcgac cgcgttcgtg gtttggatat taccattacc
     7561 actactgcga aatctgacga agaaggccgc gctctgctgg ctgcctttga cttcccgttc
     7621 cgcaagtaag gtagggttac taaatggcta agcaatcaat gaaagcacgc gaagtaaaac
     7681 gcgtagcttt agctgataaa tacttcgcga aacgcgctga actgaaagcg atcatctctg
     7741 atgtgaacgc ttccgacgaa gatcgttgga acgctgttct caagctgcag actctgccgc
     7801 gtgattccag cccgtctcgt cagcgtaacc gctgccgtca aacaggtcgt ccgcatggtt
     7861 tcctgcggaa gttcgggttg agccgtatta aggtccgtga agccgctatg cgcggtgaaa
     7921 tcccgggtct gaaaaaggct agctggtaat tgtcaccaat tgaatcacgg gagtaaagac
     7981 agatgagcat gcaagatccg atcgcggata tgctgacccg tatccgtaac ggtcaggccg
     8041 cgaacaaagc tgcggtcacc atgccttcct ccaagctgaa agtggcaatc gccaacgtgc
     8101 tgaaggaaga aggttttatt gaagatttta aagttgaagg cgacaccaag cctgaactgg
     8161 aacttactct gaagtatttc cagggcaaag ctgttgtaga aagcattcag cgtgtcagcc
     8221 gcccaggtct gcgcatctat aaacgtaaag atgagctgcc gaaagttatg gcgggtctgg
     8281 gtatcgcagt tgtttctacc tctaaaggtg ttatgactga tcgtgcagcg cgccaggctg
     8341 gtcttggtgg cgaaattatc tgctacgtag cctaatcgga ggaaaaaatg tctcgtgttg
     8401 ctaaagcacc ggtcgttgtt cctgccggcg ttgacgtaaa aatcaacggt caggttatta
     8461 cgatcaaagg taaaaacggc gagctgactc gtactctcaa cgatgctgtt gaagttaaac
     8521 atgcagataa taccctgacc ttcggtccgc gtgatggtta cgcagacggt tgggcacagg
     8581 ctggtaccgc gcgtgccctg ctgaactcaa tggttatcgg tgttaccgaa ggcttcacta
     8641 agaagctgca gctggttggt gtaggttacc gtgcagcggt taaaggcaat gtgattaacc
     8701 tgtctctggg tttctctcat cctgttgacc atcagctgcc tgcgggtatc actgctgaat
     8761 gtccgactca gactgaaatc gtgctgaaag gcgctgataa gcaggtgatc ggccaggttg
     8821 cagcggatct gcgcgcctac cgtcgtcctg agccttataa aggcaagggt gttcgttacg
     8881 ccgacgaagt cgtgcgtacc aaagaggcta agaagaagta aggtaacact atggataaga
     8941 aatctgctcg tatccgtcgt gcgacccgcg cacgccgcaa gctccaggag ctgggcgcaa
     9001 ctcgcctggt ggtacatcgt accccgcgtc acatttacgc acaggtaatt gcaccgaacg
     9061 gttctgaagt tctggtagct gcttctactg tagaaaaagc tatcgctgaa caactgaagt
     9121 acaccggtaa caaagacgcg gctgcagctg tgggtaaagc tgtcgctgaa cgcgctctgg
     9181 aaaaaggcat caaagatgta tcctttgacc gttccgggtt ccaatatcat ggtcgtgtcc
     9241 aggcactggc agatgctgcc cgtgaagctg gccttcagtt ctaaggtaga ggtgtaagat
     9301 ggctcacatc gaaaaacaag ctggcgaact gcaggaaaag ctgatcgcgg taaaccgcgt
     9361 atctaaaacc gttaaaggtg gtcgtatttt ctccttcaca gctctgactg tagttggcga
     9421 tggtaacggt cgcgttggtt ttggttacgg taaagcgcgt gaagttccag cagcgatcca
     9481 gaaagcgatg gaaaaagccc gtcgcaatat gattaacgtc gcgctgaata acggcactct
     9541 gcaacaccct gttaaaggtg ttcacacggg ttctcgcgta ttcatgcagc cggcttctga
     9601 aggtaccggt atcatcgccg gtggtgcaat gcgcgccgtt ctggaagtcg ctggggttca
     9661 taacgttctg gctaaagcat atggttccac caacccgatc aacgtggttc gtgcaactat
     9721 tgatggcctg gaaaatatga attctccaga aatggtcgct gccaagcgtg gtaaatccgt
     9781 tgaagaaatt ctggggaaat aaaccatggc aaagactatt aaaattactc aaacccgcag
     9841 tgcaatcggt cgtctgccga aacacaaggc aacgctgctt ggcctgggtc tgcgtcgtat
     9901 tggtcacacc gtagagcgcg aggatactcc tgctattcgc ggtatgatca acgcggtttc
     9961 cttcatggtt aaagttgagg agtaagagat gcgtttaaat actctgtctc cggccgaagg
    10021 ctccaaaaag gcgggtaaac gcctgggtcg tggtatcggt tctggcctcg gtaaaaccgg
    10081 tggtcgtggt cacaaaggtc agaagtctcg ttctggcggt ggcgtacgtc gcggtttcga
    10141 gggtggtcag atgcctctgt accgtcgtct gccgaaattc ggcttcactt ctcgtaaagc
    10201 agcgattaca gccgaagttc gtctgtctga cctggctaaa gtagaaggcg gtgtagtaga
    10261 cctgaacacg ctgaaagcgg ctaacattat cggtatccag atcgagttcg cgaaagtgat
    10321 cctggctggc gaagtaacga ctccggtaac tgttcgtggc ctgcgtgtta ctaaaggcgc
    10381 tcgtgctgct atcgaagctg ctggcggtaa aatcgaggaa taagtagcag atggctaaac
    10441 aaccgggatt agattttcaa agtgccaaag gtggcttagg cgagctgaaa cgcagactgc
    10501 tgtttgttat cggtgcgctg attgtgttcc gtattggctc ttttattccg atccctggta
    10561 ttgatgccgc tgtacttgcc aaactgcttg agcaacagcg aggcaccatc attgagatgt
    10621 ttaacatgtt ctctggtggt gctctcagcc gtgcttctat ctttgctctg gggatcatgc
    10681 cgtatatttc ggcgtcgatc attatccagc tgctgacggt ggttcaccca acgttggcag
    10741 aaattaagaa agaaggggag tctggtcgtc gtaagatcag ccagtacacc cgttacggta
    10801 ctctggtgct ggcaatattc cagtcgatcg gtattgctac cggtctgccg aatatgcctg
    10861 gtatgcaagg cctggtgatt aacccgggct ttgcattcta cttcaccgct gttgtaagtc
    10921 tggtcacagg aacgatgttc ctgatgtggt tgggcgaaca gatcactgaa cgaggtatcg
    10981 gcaacggtat ttcaatcatt atcttcgccg gtattgtcgc gggactcccg ccagccattg
    11041 cccatactat cgagcaagcg cgtcaaggcg acctgcactt cctcgtgttg ctgttggttg
    11101 cagtattagt atttgcagtg acgttctttg ttgtatttgt tgagcgtggt caacgccgca
    11161 ttgtggtaaa ctacgcgaaa cgtcagcaag gtcgtcgtgt ctatgctgca cagagcacac
    11221 atttaccgct gaaagtgaat atggcggggg taatcccggc aatcttcgct tccagtatta
    11281 ttctgttccc ggcgaccatc gcgtcatggt tcgggggcgg tactggttgg aactggctga
    11341 caacaatttc gctgtatttg cagcctgggc aaccgcttta tgtgttactc tatgcgtctg
    11401 caatcatctt cttctgtttc ttctacacgg cgttggtttt caacccgcgt gaaacagcag
    11461 ataacctgaa gaagtccggt gcatttgtac caggaattcg tccgggagag caaacggcga
    11521 agtatatcga taaagtaatg acccgcctga ccctggttgg tgcgctgtat attaccttta
    11581 tctgcctgat cccggagttc atgcgtgatg caatgaaagt accgttctac ttcggtggga
    11641 cctcactgct tatcgttgtt gtcgtgatta tggactttat ggctcaagtg caaactctga
    11701 tgatgtccag tcagtatgag tctgcattga agaaggcgaa cctgaaaggc tacggccgat
    11761 aattggtcgc ccgagaagtt acggagagta aaaatgaaag ttcgtgcttc cgtcaagaaa
    11821 ttatgccgta actgcaaaat cgttaagcgt gatggtgtca tccgtgtgat ttgcagtgcc
    11881 gagccgaagc ataaacagcg ccaaggctga ttttttcgca tatttttctt gcaaagttgg
    11941 gttgagctgg ctagattagc cagccaatct tttgtatgtc tgtgcgtttc catttgagta
    12001 tcctgaaaac gggcttttca gcatggaacg tacatattaa atagtaggag tgcatagtgg
    12061 cccgtatagc aggcattaac attcctgatc ataagcatgc cgtaatcgca ttaacttcga
    12121 tttatggcgt cggcaagacc cgttctaaag ccatcctggc tgcagcgggt atcgctgaag
    12181 atgttaagat cagtgagctg tctgaaggac aaatcgacac gctgcgtgac gaagttgcca
    12241 aatttgtcgt tgaaggtgat ctgcgccgtg aaatcagcat gagcatcaag cgcctgatgg
    12301 atcttggttg ctatcgcggt ttgcgtcatc gtcgtggtct cccggttcgc ggtcagcgta
    12361 ccaagaccaa cgcacgtacc cgtaagggtc cgcgcaaacc gatcaagaaa taatcggggt
    12421 gattgaataa tggcaaaggc accaattcgt gcacgtaaac gtgtaagaaa acaagtctct
    12481 gacggcgtgg ctcatatcca tgcttctttc aacaacacca tcgtgactat cactgatcgt
    12541 cagggtaacg cgttgggttg ggcaacagcc ggtggttccg gtttccgtgg ttctcgcaaa
    12601 tccactccgt ttgcagctca ggttgcagca gagcgttgcg ctgacgccgt gaaagaatac
    12661 ggcatcaaga atctggaagt tatggttaaa ggtccgggtc caggccgcga atctactatt
    12721 cgtgctctga acgccgcagg tttccgcatc actaacatta ctgatgtgac tccgatccct
    12781 cataacggtt gtcgtccgcc gaaaaaacgc cgcgtataac gcctcgtttt ccaggtttgt
    12841 tggagaaaga aaatggcaag atatttgggt cctaagctca agctgagccg tcgtgagggc
    12901 accgacttat tccttaagtc tggcgttcgc gcgatcgata ccaagtgtaa aattgaacaa
    12961 gctcctggcc agcacggtgc gcgtaaaccg cgtctgtctg actatggtgt gcagttgcgt
    13021 gaaaagcaaa aagttcgccg tatctatggt gtgctggagc gtcagttccg taactactac
    13081 aaagaagcag cacgtctgaa aggcaacact ggtgaaaacc tgttggctct gctggaaggt
    13141 cgtctggaca acgttgtata ccgtatgggc ttcggtgcca ctcgtgcaga agcacgtcag
    13201 ctggttagcc ataaagcaat tatggtaaac ggtcgtgttg ttaacatcgc ttcttatcag
    13261 gttagtccga atgacgttgt aagcattcgt gagaaagcga agaagcagtc tcgcgtgaaa
    13321 gccgctctgg agctggctga gcagcgtgaa aagccaacct ggctggaagt tgatgctggc
    13381 aagatggaag gtacgtttaa gcgtaagccg gagcgttctg atctgtctgc ggacattaac
    13441 gaacacctga tcgtcgagct ttactccaag taaagcttag taccaaagag aggacacaat
    13501 gcagggttct gtgacagagt ttctaaaacc gcgcctggtt gatatcgagc aagtgagttc
    13561 gacgcacgcc aaggtgaccc ttgagccttt agagcgtggc tttggccata ctctgggtaa
    13621 cgcactgcgc cgtattctgc tctcatcgat gccgggttgc gcggtgaccg aggttgagat
    13681 tgatggtgta ctacatgagt acagcaccaa agaaggcgtt caggaagata tcctggaaat
    13741 cctgctcaac ctgaaagggc tggcggtgag agttcagggc aaagatgaag ttattcttac
    13801 cttgaataaa tctggcattg gccctgtgac tgcagccgat atcacccacg acggtgatgt
    13861 cgaaatcgtc aagccgcagc acgtgatctg ccacctgacc gatgagaacg cgtctattag
    13921 catgcgtatc aaagttcagc gcggtcgtgg ttatgtgccg gcttctaccc gaattcattc
    13981 ggaagaagat gagcgcccaa tcggccgtct gctggtcgac gcatgctaca gccctgtgga
    14041 gcgtattgcc tacaatgttg aagcagcgcg tgtagaacag cgtaccgacc tggacaagct
    14101 ggtcatcgaa atggaaacca acggcacaat cgatcctgaa gaggcgattc gtcgtgcggc
    14161 aaccattctg gctgaacaac tggaagcttt cgttgactta cgtgatgtac gtcagcctga
    14221 agtgaaagaa gagaaaccag agttcgatcc gatcctgctg cgccctgttg acgatctgga
    14281 attgactgtc cgctctgcta actgccttaa agcagaagct atccactata tcggtgatct
    14341 ggtacagcgt accgaggttg agctccttaa aacgcctaac cttggtaaaa aatctcttac
    14401 tgagattaaa gacgtgctgg cttcccgtgg actgtctctg ggcatgcgcc tggaaaactg
    14461 gccaccggca agcatcgctg acgagtaacc ggatcacagg ttaaggtttt actgagaagg
    14521 ataaggtcat gcgccatcgt aagagtggtc gtcaactgaa ccgcaacagc agccatcgcc
    14581 aggctatgtt ccgcaatatg gcaggttcac tggttcgtca tgaaatcatc aagacgactc
    14641 tgcctaaagc gaaagagctg cgccgcgtag ttgagccgct gattactctt gccaagactg
    14701 atagcgttgc taatcgtcgt ctggcattcg cccgtactcg tgataacgag atcgtggcaa
    14761 aactgtttaa cgaactgggc ccgcgtttcg cgagccgtgc cggtggttac actcgtattc
    14821 tgaagtgtgg cttccgtgca ggcgacaacg cgccgatggc ttacatcgag ctggttgatc
    14881 gttcagagaa agcagaagct gctgcagagt aatctgaagc aacgtaaaaa aacccgcccc
    14941 ggcgggtttt tttatacccg tagcatcccc acttatctac aatagctgta ctctttttgt
    15001 tcatcccctg gagtatttat gtggttactt gaccagtggg cagagcgcca tatagcagaa
    15061 gcgcaagcga aaggtgagtt tgataacctg gcaggtagcg gcgaaccatt gatactggat
    15121 gatgattctc atgtgccacc ggaattacgt gcggggtatc gcttgctgaa gaatgccggt
    15181 tgcttaccgc cagaacttga gcaacggagg gaagcaatcc agcttctgga tatcctcaaa
    15241 ggtatccgtc acgatgatcc gcaatatcaa gaggttagcc gtcgattgtc attactggaa
    15301 ttgaagctgc gacaagctgg attgagtacc gattttttac gcggcgatta tgctgacaag
    15361 ttgttgaata aaatcaacga taactagtgg agtatgtatg tatcgcattg gtgagctggc
    15421 aaaaatggcg gaagtaacac ccgacacgat tcgttattac gaaaagcagc agatgatgga
    15481 gcatgaagtg cgtaccgaag gtgggtttcg cctgtatacc gaaagcgatc tccagcgatt
    15541 gaaatttatc cgccatgcca gacaactagg tttcagtctg gagtcgatcc gcgagttgct
    15601 gtcgatccgt atcgatcctg aacaccatac ctgtcaggag tcaaaaggca ttgtgcagga
    15661 aagattgcag gaagtcgaag cacggatagc cgagttgcag agtatgcagc gttccttgca
    15721 acgccttaac gatgcctgtt gtgggaccgc ccatagcagt gtttattgct cgattcttga
    15781 agctcttgaa caaggggcga gtggcgttaa gagtggctgt tgattttttg cactggcagg
    15841 attacactca cgccgttaaa taaccaactg gagtttttat gagccgatat cagcatacta
    15901 aagggcagat aaaggataat gcgatagaag cattactaca tgatccctta ttccgacagc
    15961 gcgtagagaa aaataagaag gggaaaggca gttacatgcg aaaaggaaaa catggcaatc
    16021 gggggaactg ggaggccagt ggcaagaaag taaatcactt ttttaccact ggtcttctgc
    16081 tttcaggggc ttgttaagag cggttattct gctctttcag caaatcacga atttctgtca
    16141 gtaatacttc ttctttagtt ggtgctggtg cggctgctgg ttcttctttt ttccgattca
    16201 gtttgttgat tagcttaatc gccataaaga tggcaaaggc cacaatcaga aaatcaaaga
    16261 cgttttgaat gaagacaccg taatgcatca caacagcagg gatatccccc tgcgcatcgc
    16321 gtagcgtgac agcaaactgt ttaaagtcga tcccgccaat taataagccc agtggtggca
    16381 tgatgatatc ggcaaccagt gaagatacaa tcttcccgaa tgccgcaccg ataatgacac
    16441 ccaccgccaa atccaccacg ttcccgcgca tcgcaaattc gcgaaattct ttaataatgc
    16501 tcatgttatt ctccctatga agccgacaac cataagtcta acaaatgtta agccattttc
    16561 ctgccaggaa tgaatttaat taatccttga taatcaaagg gaaataataa ggcgtcatta
    16621 gacgccttat taattacaag aagaaagggc ttggctggaa gagtctttcg acgtcggtaa
    16681 taaatttttt atctgtgagg aacataatta cgtgatcgcc ttgctcaatg cgcagattgt
    16741 cattggcaat catcacgtcg tttccacgta ccaccgctcc aataatcgtt cctggcggta
    16801 gcttgatttc gtcaatgact ctgccgacaa cgcgtgaggt gctttcatca ccgtgagcaa
    16861 cggcttcaat agcttctgct acgccgcggc gcaatgagga aacaccaaca atatctgctt
    16921 ttcgcacatg gctaagcaac gcagaaatag ttgcttgttg tggtgaaatc gcaatatcga
    16981 tcacgctccc ctgaaccaga tccacataag cgcgacgctg gatcaatacc atcacctttt
    17041 tcgcacccat acgtttggca agcatggcgg acatgatatt ggcctcgtca tcgttggtga
    17101 cggcaataaa cagatcaact tgatcgatat gttcttcggc cagcagttct tgatccgacg
    17161 catcaccaaa aaagacgatc gtattctgca acttttccgc cagttcggca gcgcgctgct
    17221 gattacgttc gatgagttta acgctgtaat ctttttccag acgacgcgcc agccctgcac
    17281 cgatattacc gccaccaacc agcatgatcc gcttatacgg tttttccagt cgctgtaatt
    17341 cactcatcac cgcgcggata tgctgtgaag cggcaataaa gaacacttca tcaccagcct
    17401 caacaatggt cgaaccttgc ggacgaatgg ggcgatcgtg gcggaaaatt gctgccacac
    17461 gagtatcgat atgtggcata tgttcgcgca tggtcgaaag tgcattacca atcagcgggc
    17521 cgccataata ggctttaacc acagcgaggc tgactttacc ctcagcgaag ttcaccacct
    17581 gcaatgcgcc gggatactca atcagtcggt aaatattatc gataaccaac tgctctggtg
    17641 cgatcagatg atcaatcggt acagcatctg aatgaaatag cttatcggca tcgcgtacgt
    17701 agtctggtga gcggatacga gcgatgcgat taggggtgtt gaaaagtgag taggctacct
    17761 ggcaggcaac catattggtt tcatctgaac tggttacagc aaccagcata tcggcgtcgt
    17821 cggcacctgc ctcccgcaat acgcgtggat gagagccatg cccctgcacg actcgcaggt
    17881 caaatttatc ctgcaaggtt cgcagacgct caccgttggt atcgacaaca gtgatatcgt
    17941 tgttctcgcc aaccaggttt tccgccagtg tgccgccaac ctggccggca cccagaatga
    18001 taattttcat cagtcgcgac ccgttatctc atcacttttt gattagctta gcgtaaaaga
    18061 agccgtcgcc ctcttcggca ccaggcagat tttgtttacc cggttgctct ggtgttcctg
    18121 tttcgcaaag ttcggcatca gcggtacgtt gcaaaaaggc tttaatctgc aggctattct
    18181 cttccggtaa caccgaacag gtggcataga ccagggttcc accggatttt aaatgcggcc
    18241 aaatggcgtc gagaatttca gactgcaatt gcgcgagttc tgggatatcg cgatcgcgac
    18301 gtaaccattt aatatccgga tggcgacgaa tcacaccggt tgctgaacaa ggcgcatcta
    18361 ataaaatgcg atcaaactgt tgctcgccac accattggga agggtaacgg ccatcacctt
    18421 gtttcacggt tgccttcata ccaaggcgtt ttaaattgtc gtaaacgcga gagaggcgct
    18481 gttcgtcaat atcaaccgca acaacctgcg cttctggtgc cacttcaagg atatgcgttg
    18541 ttttaccgcc gggggcggca caaagatcca gaatgtgttc accgttttgt ggcgcaagcc
    18601 aggtcatgca accttgtgct gatgcatcct gaacggtaac ccatccgtct tcaaaaccag
    18661 gtagcgcatg aacaggtgca ggtgtttcca gccgtacagc atcagggtaa tccgcatgcg
    18721 ggaaaccttt cattcctgct tcatccagca atgcaagcca ggtgtcgcgg gaatgatgcg
    18781 tacgattaat acgcagccac attggcggac gctggttatt ggcttcgacg atggattgcc
    18841 actgctctgg atacgctttt tgcagacgct tcagcaacca ggaaggatgc agataacgtg
    18901 catcactggt attaaactcg gctaataact cttcttgctg acgctggaac tggcgtaata
    18961 caccgttaat caacccttta agttgcggac gcttaattgc gatagcgcct tcaaccgttt
    19021 cagccagcgc agcatgaggt ggaatgcggg tataaagcag ttgatacaaa ccaaccataa
    19081 tcaggtaatg cacggtccgc tgtttgccgg tcatcggacg ggccattgac ttattaatca
    19141 gccagtctaa ctgcgaaagc gtacgcagta cgccaaagca caactcttga agaagtgctt
    19201 tgtctttatc ggaaactttt tgctggagcg gtggcagaat gttgcttaat gattgccctt
    19261 gctcgacgac ttgttcaacg gcctgggccg ccatgctacg taaattacgt tgttttttca
    19321 taagtataaa aatgcccggc aagaccgggc ttagaagagt ggactatcag accagacggt
    19381 tgcccggaac aaaccattcc cgacgagagt tcaggaggtc ttgcgcgctc atcgctttct
    19441 tacccgcagg ttgtaacgag agcaggttca ggatgccatc accagtcgca acctgaatgc
    19501 cttgtttgtt ggcttcaagg atcgttcctg gtgcagcgtt ggttgccgta tcaatgaccg
    19561 atgctttcca gactttaacc ggctgtcctt caatttccac ccagctcatt ggccatggat
    19621 tgaaagcgcg aatgcagcgt tcaagctgtg ctgccgaaag tgaccagtca atacgcgctt
    19681 cttctttact caacttctcg gcgtaagtga caagagtttc gtcctgaact tctggtttcg
    19741 ccgtgccgtc tgccaattgt ttcaacgtgg tgataagccc ttgtgggcca agctctgcca
    19801 gcttgtcgta cagcgtacca ctggtatctt ctgcagtaat cgggcaggag agcttataga
    19861 gcatgtcacc ggtgtctaaa ccaacatcca tttgcataat ggtcacacca gtttctgcat
    19921 cacccgccca tagtgagcgt tggattggtg cagcaccgcg ccagcgtggc agcagtgaac
    19981 catgaacgtt gatacagcca agacgcggca tctccagcac tgctttaggc agaattaagc
    20041 cataggcgac gacaaccata acatcagcct gtagatcggc gaccagttgc tggttttctt
    20101 gtggacgcag ggaaacaggt tgaaaaacgg gcagaccttt ttcctcagcc agaactttaa
    20161 ccgggctggg catcagtttt ttaccgcgtc ctgccggtcg gtctggctgg gtgaacacgc
    20221 caacgacgtt atgaccagaa gacaacagcg cgtcgagatg acgcgctgca aagtcaggtg
    20281 tacccgcaaa aataatacgt agtgattctg acacgttagt tcttatcctt aagcccgggc
    20341 tttcagacga tccagttttt caactttctg acgaatacgt tgttgtttca gcggtgacag
    20401 ataatccata aacagtttgc cgaccaggtg atccatctca tgctgaatac agatggctaa
    20461 cagaccatct gcctccagtt caaatggttt accgtcgcgg tccagggcgc gaattttaac
    20521 tttctctgcg cgtggcacta aagcacgttg ttcagggatc gacaggcaac cttcttcaat
    20581 gcctgtttcg ccgctttttt ctaaaagctc cggattgatt aacaccagcc gttcgtcacg
    20641 gttttccgaa acatcaataa caatgatacg ttgatggata tcaacctggg ttgccgccag
    20701 gccaatacct tcttctgcgt acatcgtctc gaacatatca tcgacgatac gctgaatttc
    20761 tgcattcact tcttctaccg gtttagcaac tttgcgaagc cgctcgtccg gaatatgtaa
    20821 cacttgcaaa actgacataa atctccagag atgtgttcag gagttagaaa gattatttct
    20881 tctattctag acaaatcccc ctctgattga cagcatcact gaccaatcgc aaagattgct
    20941 aaggctgctt atggcaggga gataaggatg gtcgatacag aaatttggct gcgtttaatg
    21001 agtatcagca gcttgtacgg cgatgatatg gtccgtatag ctcactggct ggcaagacag
    21061 tcgcatattg atgcggttgt attgcagcaa acagggctta cattgcggca ggcacaacgc
    21121 tttctttcat ttccgcggaa gagtatcgaa agctcacttt gttggttgga gcaacccaac
    21181 catcatttaa tccctgcgga cagcgaattt tatcctcctc aacttctggc gacgacagat
    21241 taccccggtg cactgtttgt tgaaggagaa ctgcacgcgc tgcattcatt tcagcttgcc
    21301 gtagtgggga gtcgggcgca ttcatggtat ggcgagcgat ggggacgatt attttgcgaa
    21361 actctggcga agcatggagt gacaattacg agtggactgg cgcgtggaat cgatggtgta
    21421 gcgcataaag cagccttaca ggtaaatggc gtcagcattg ctgtattggg gaatggactt
    21481 aataccattc atccccgccg tcatgcccga ctggctgcca gtctgcttga acaggggggc
    21541 gctctcgtct cggaatttcc cctcgatgtt ccaccccttg cttacaattt cccacgaaga
    21601 aatcgcatta tcagtggtct aagtaaaggt gtactggtgg tggaagcggc tttgcgtagt
    21661 ggttcgctgg tgacagcacg ttgtgcgctt gagcaggggc gagaagtttt tgccttgcca
    21721 ggtccaatag ggaatccggg aagcgaaggg cctcactggt taataaaaca aggtgcgatt
    21781 cttgtgacgg aaccggaaga aattctggaa aacttgcaat ttggattgca ctggttgcca
    21841 gacgcccctg aaaattcatt ttattcacca gatcaggaag acgtggcatt gccatttcct
    21901 gagctcctgg ctaacgtagg agatgaggta acacctgttg acgtcgtcgc tgaacgtgcc
    21961 ggccgacctg tgccagaggt agttactcaa ctactcgaac tggagttagc aggatggatc
    22021 gcagctgtac ccggcggcta tgtccgattg aggagggcat gccatgttcg acgtactaat
    22081 gtatttgttt gaaacctata ttcacacaga agctgagttg cgtgtggatc aagacaaact
    22141 tgaacaggat cttaccgacg caggatttga tcgagaagat atctacaatg ccctgctttg
    22201 gctggaaaag cttgcggatt atcaggaagg actggcagaa ccgatgcaac tggcctctga
    22261 tccactctcc atgcgtattt atacccctga ggagtgtgaa agactggatg ccagctgtcg
    22321 tggttttctg cttttccttg agcaaattca ggtgcttaac cttgaaaccc gtgaaatggt
    22381 gatagagcga gtgctcgcgc tggataccgc agagttcgac ctggaagacc tgaaatgggt
    22441 aatcctgatg gtgttgttca atattccggg ctgcgaaaat gcgtaccagc aaatggaaga
    22501 attactcttt gaagtgaatg aaggtatgct gcattaatca tttctttaat tcagcataag
    22561 ttgttatggc gaaatcagca ctgttcacgg tgcgtaataa tgagtcctgc ccaaagtgcg
    22621 gggctgaact ggttattcga tccgggaaac acggtccgtt tcttggatgc tcacagtatc
    22681 cggcgtgtga ctacgtccgt cctctgaaat cttcagcgga tggacatatc gtcaaagttc
    22741 tggaggggca ggtttgccct gcatgtggcg caaatctggt attacgccag ggacgctttg
    22801 gtatgtttat tggttgcatt aactaccctg aatgcgaaca taccgaactt atcgataaac
    22861 cggacgaaac agcaattaca tgtccccaat gccggacggg ccatctggtc cagcgccgct
    22921 cccgttatgg caaaacattt cactcctgtg atcgctaccc ggagtgtcaa tttgccatta
    22981 acttcaaacc catagctgga gaatgccctg agtgtcatta tccgctactc atcgaaaaga
    23041 aaaccgcgca gggtgtaaaa cacttttgtg ccagtaaaca atgtggaaag ccggtttcgg
    23101 cggaataata acgtgaataa taacctgcaa ggagacgcta tcgcagctgc gatagatgtt
    23161 ctcaatgaag aacgtgtcat cgcctatcca acggaagccg ttttcggtgt tgggtgcgat
    23221 cctgatagcg aaacagcagt gatgcgactg ttggagttaa aacagcgtcc ggttgataag
    23281 gggctgattt taatcgcagc aaattacgag cagcttaaac cctatattga tgacaccatg
    23341 ttgactgacg tgcagcgtga aaccattttt tcccgctggc caggtcctgt cacctttgtc
    23401 tttcccgcgc ctgcgacaac accgcgctgg ttgacgggcc gctttgattc gcttgctgta
    23461 cgagtcaccg accatccgtt ggtggttgct ttgtgccagg cttatggtaa accgctggtt
    23521 tctaccagtg ccaacttgag tggattgcca ccttgtcgaa cagtagacga agttcgcgca
    23581 caatttggcg cggcgttccc ggttgtgcct ggtgaaacgg gggggcgttt aaatccttca
    23641 gaaatccgcg atgccctgac gggtgaactg tttcgacagg ggtaacataa tggaaaccta
    23701 tgctgttttt ggtaatccga tagcccacag caaatcgcca ttcattcatc agcaatttgc
    23761 tcagcaactg aatattgaac atccctatgg gcgcgtgttg gcacccatca atgatttcgt
    23821 caacacactg aacgctttct ttagtgctgg tggtaaaggt gcgaatgtga cggtgccttt
    23881 taaagaagag gcttttgcca gagcggatga gcttactgaa cgcgcagcgt tggctggtgc
    23941 tgttaacacc ctcaagcggt tagaagatgg acgcttgctg ggtgacaata ccgatggtgt
    24001 aggcttgtta agcgatctgg aacgtctgtc ttttatccgt cctggtttac gtattctgct
    24061 catcggcgca ggtggagcat ctcgcggcgt actactgcca cttctttccc tggactgtgc
    24121 ggtgacaatc actaatcgga cggtatcccg agcggaagag ttggctaaat tgtttgcgca
    24181 cactggcagt attcaggcgt tgggtatgga cgaactggaa ggtcatgagt ttgatctcat
    24241 tattaatgcc acatccagtg gcatcagtgg tgatattccg gcgatcccgt catcgctcat
    24301 tcatccaggc atttattgct atgacatgtt ctatcagaaa ggaaaaactc cttttttggc
    24361 atggtgtgag cagcgaggct caaagcctac tgctgatggt ttaggaatgc tggtggcaca
    24421 ggcggctcat gccttccttc tttggcatgg cgttctgcct gatgtagaac cagttataaa
    24481 gcaattgcag gaggaattgt ccgcgtgaat caggccatcc agtttccgga cagagaagag
    24541 tgggtcgaga ataaaaaatg tgtatgtttt cccgctctcg tgaatggcat gcaactgaca
    24601 tgtgcgatct ctggcgatag cctggcgtat cgctttacag gagatacgcc agagcagtgg
    24661 ttagcgagtt ttcgtcagca tcgctgggac ttggaagaag aagcggaaaa cttaattcag
    24721 gaacaaagtg aagatgatca aggctgggtc tggttaccct gatccagata ttcgtccttc
    24781 catttcacgt aattattcgc ggaatagcgt aacccagcct tctcttcatc acttaacggg
    24841 cggatctgtt tgacgggact accgagatac agatatccgc tctccagccg tttattttgt
    24901 gggaccagac tacccgcacc aatcatcaca tcatcttcta ctattgcgcc atcaagtaaa
    24961 attgagccca tcccaaccaa aactcgattg ccaatggtgc agccgtggag catcaccttg
    25021 tgaccaacag tgacatcttc gccaatggtt aatgggttgc catctgggtt gtacgaggat
    25081 ttatgagtga catgcaacat actgccatcc tggatattgg tgcgtgctcc gatctgtaca
    25141 taatgtacat ctccacgaat cacaacgagc ggccagatcc ccacatcatc agccagacga
    25201 acgtcaccaa tcacgacact gctatcgtcg atcattacgc gctgaccgat ttgtggaaaa
    25261 agatcgcggt atgggcgtaa aacatcagac atacttacct cagcaataaa tgatttacta
    25321 atgactttgg gggcattatt ggccttgtgc aagtctttta gtatgcaaaa aagcaccgtt
    25381 ttgtgtgcga atgcagcaaa aagggtgaaa aaacaacaaa cagaaaaaaa gatcaaaaaa
    25441 gtacttgtgc aaaaaattgg gatccctata atgcgcctcc gttgagacga caacgtgaaa
    25501 cacttcacaa gatggtcgta acaacgaaga gaaaaaatcc tg
//
LOCUS       DBFIFI010000050        25327 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.050, whole genome shotgun sequence.
ACCESSION   DBFIFI010000050 DBFIFI010000000
VERSION     DBFIFI010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25327
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.050"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            184..1323
                     /gene="wza"
                     /locus_tag="N7B39_001971"
     CDS             184..1323
                     /gene="wza"
                     /locus_tag="N7B39_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HGU2421962.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            1329..1772
                     /gene="wzb"
                     /locus_tag="N7B39_001972"
     CDS             1329..1772
                     /gene="wzb"
                     /locus_tag="N7B39_001972"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HGU2421963.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            1775..3937
                     /gene="wzc"
                     /locus_tag="N7B39_001973"
     CDS             1775..3937
                     /gene="wzc"
                     /locus_tag="N7B39_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HGU2421964.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQSSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMEDQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQILKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            4030..4869
                     /gene="wcaA"
                     /locus_tag="N7B39_001974"
     CDS             4030..4869
                     /gene="wcaA"
                     /locus_tag="N7B39_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HGU2421965.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACTVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            4872..5360
                     /gene="wcaB"
                     /locus_tag="N7B39_001975"
     CDS             4872..5360
                     /gene="wcaB"
                     /locus_tag="N7B39_001975"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HGU2421966.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            5357..6574
                     /gene="wcaC"
                     /locus_tag="N7B39_001976"
     CDS             5357..6574
                     /gene="wcaC"
                     /locus_tag="N7B39_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HGU2421967.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKGVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQALFGTTLAEFSQRSRVAYSGQ
                     QMLEEYVNFYQNL"
     gene            6549..7766
                     /gene="wcaD"
                     /locus_tag="N7B39_001977"
     CDS             6549..7766
                     /gene="wcaD"
                     /locus_tag="N7B39_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HGU2421968.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKITQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGICAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            7777..8523
                     /gene="wcaE"
                     /locus_tag="N7B39_001978"
     CDS             7777..8523
                     /gene="wcaE"
                     /locus_tag="N7B39_001978"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HGU2421969.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGIAMAQGKFALFLNSGDIFHQDAAYF
                     VRKLKMQKDNVMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWVELSWHLRQRTTSKTKALYNKS"
     gene            8539..9087
                     /gene="wcaF"
                     /locus_tag="N7B39_001979"
     CDS             8539..9087
                     /gene="wcaF"
                     /locus_tag="N7B39_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HGU2421970.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYVWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRKRVETE"
     gene            9116..10237
                     /gene="gmd"
                     /locus_tag="N7B39_001980"
     CDS             9116..10237
                     /gene="gmd"
                     /locus_tag="N7B39_001980"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HGU2421971.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            10240..11205
                     /gene="fcl"
                     /locus_tag="N7B39_001981"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             10240..11205
                     /gene="fcl"
                     /locus_tag="N7B39_001981"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HGU2421972.1"
                     /translation="MSRQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            11208..11687
                     /gene="gmm"
                     /locus_tag="N7B39_001982"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             11208..11687
                     /gene="gmm"
                     /locus_tag="N7B39_001982"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HGU2421973.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            11684..12907
                     /gene="wcaI"
                     /locus_tag="N7B39_001983"
     CDS             11684..12907
                     /gene="wcaI"
                     /locus_tag="N7B39_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HGU2421974.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            12910..14346
                     /gene="cpsB"
                     /locus_tag="N7B39_001984"
     CDS             12910..14346
                     /gene="cpsB"
                     /locus_tag="N7B39_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HGU2421975.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPENDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEDAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            14539..15909
                     /gene="cpsG"
                     /locus_tag="N7B39_001985"
                     /gene_synonym="manB"
     CDS             14539..15909
                     /gene="cpsG"
                     /locus_tag="N7B39_001985"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HGU2421976.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDARLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPSSGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            15964..17358
                     /gene="wcaJ"
                     /locus_tag="N7B39_001986"
     CDS             15964..17358
                     /gene="wcaJ"
                     /locus_tag="N7B39_001986"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HGU2421977.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKLGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            17360..18838
                     /gene="wzxC"
                     /locus_tag="N7B39_001987"
     CDS             17360..18838
                     /gene="wzxC"
                     /locus_tag="N7B39_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HGU2421978.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDLI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYVLGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            19018..20298
                     /gene="wcaK"
                     /locus_tag="N7B39_001988"
     CDS             19018..20298
                     /gene="wcaK"
                     /locus_tag="N7B39_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HGU2421979.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQVYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGVMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLSEAVSRERQTGMQMVQSVLERIGEVK"
     gene            20295..21515
                     /gene="wcaL"
                     /locus_tag="N7B39_001989"
     CDS             20295..21515
                     /gene="wcaL"
                     /locus_tag="N7B39_001989"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HGU2421980.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THVAWTKYNLAARTRWLQDEPTGKVAKLSHRASQTLRGIHRKNTWQALNLKRYGAESW
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGVIHGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            21526..22920
                     /gene="wcaM"
                     /locus_tag="N7B39_001990"
     CDS             21526..22920
                     /gene="wcaM"
                     /locus_tag="N7B39_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HGU2421981.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARAFPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTI
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITQDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGNYLSIPQNF
                     RVNNIQLDNTHLAYKLRGLQISAGNAVSFVALTNIEMKRASLELHNKPQHLFLRNIKV
                     MQESSVGPALIMNFDMRKDVRGVFMAKEETLLSLANVHAVNEKGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            23078..24073
                     /locus_tag="N7B39_001991"
     CDS             23078..24073
                     /locus_tag="N7B39_001991"
                     /EC_number="5.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,
                     octacis-undecaprenol 4-epimerase"
                     /protein_id="HGU2421982.1"
                     /translation="MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQ
                     IGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLAAMEKNGVK
                     NIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
                     PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGY
                     EVYNYVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAV
                     SSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
     gene            24316..25209
                     /gene="galF"
                     /locus_tag="N7B39_001992"
     CDS             24316..25209
                     /gene="galF"
                     /locus_tag="N7B39_001992"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HGU2421983.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
BASE COUNT         6241 a         6293 c         6861 g         5932 t
ORIGIN      
        1 gcgtgtctgg atgcctgaaa gaccagtctg aattattctg tcaatagcct gcggatgaaa
       61 tcaatttttt taggactgat gccagttaaa tttttattca attaactgca aggtaaatgt
      121 gcatggcgac attgctatta atagtgcaca ggataattac tctgccaaag tgataaataa
      181 acaatgatga aatccaaaat gaaattgatg ccattattgg tgtcagtaac cttgataagc
      241 ggttgcacag tacttccggg cagcaatatg tcgacgatgg gcaaagacgt catcaaacag
      301 caggacgctg atttcgatct cgacaaaatg gtgaatgttt atccgctgac cccgcgcctg
      361 attgaccaat tacgcccacg cccgaatgta gcgcgcccca atatgacgct ggaaagtgag
      421 atcgcgaatt accagtatcg cgtcgggccg ggggacgttc ttaatgtcac cgtctgggat
      481 cacccggaac tcaccacgcc agccggtcag taccgcagct ccagcgacac cggcaactgg
      541 gtacagcctg acggcactat gttttacccg tatatcggca aggttcacgt agtcgggaaa
      601 acgctcgctg aaatccgcag tgatattacc gggcgcttag cgacgtacat cgctgacccg
      661 caggtggacg ttaatatcgc cgccttccgc tcacaaaagg cctatatctc aggtcaggtg
      721 aataaatctg gtcaacaggc gatcaccaac gtaccactga ccattctcga cgccatcaac
      781 gccgcaggtg gcctgaccga caccgctgac tggcgcaacg tggtgctaac acacaatggt
      841 cgtgaagagc gaatttcttt gcaggcgctg atgcaaaacg gcgacctcaa tcagaaccga
      901 ctgctttacc ccggcgatat tctctacgtg ccgcgtaatg atgatctgaa agtgtttgtg
      961 atgggtgaag tgaagaaaca gagcaccctg aaaatggact ttagcggcat gaccctgacc
     1021 gaagccctgg gcaatgctga aggcatcgac atgaccacct ccaacgccag cggcatcttt
     1081 gtcattcgtc cgctgaaagg cgagggcggg cgtaacggca aaattgccaa tatctaccag
     1141 ctggatatgt ccgatgccac gtcgctggtg atggcgacag aattccgcct gcaaccttat
     1201 gacgtggtgt atgtcaccac cgccccggtt tcccgctgga accgtctgat caatcagttg
     1261 ctgccaacta ttagcggtgt ccgttacatg acggatacag ccagcgacat tcataactgg
     1321 taatcgtcat gtttaacaac atcttagttg tctgtgtcgg caatatttgc cgttccccga
     1381 cggcggaacg cttactgcaa cgttatcacc cggagctgaa agtggagtcc gctggactcg
     1441 gcgcgctggt cggtaagggc gctgatccta ccgctatcag cgtcgccgca gaacatcaac
     1501 tgtctctgga aggccactgt gcacgacaaa tcagccgccg cctgtgtcgc aactatgacc
     1561 tgattttgac catggaaaag cgccatatcg aacgcttatg cgagatggca ccggagatgc
     1621 gcggcaaagt gatgctgttt ggtcactggg ataacgaatg tgaaatcccc gatccgtatc
     1681 gcaaaagccg ggaaacgttt gcagcggtgt acacattact tgaacggtct gcccgccagt
     1741 gggcgcaggc attgaacgca gagcaggtat aagaatgaca gaaaaagtaa aacaacatgc
     1801 cgctccggta acgggcagtg atgaaatcga tattggtcgc ctggtcggca ccgtcattga
     1861 agcgcgctgg tgggtgattg gcatcaccgc tgtattcgcc ctttgtgccg tggtttacac
     1921 cttcttcgcc acgccgattt atagtgccga cgcactggta caaatcgagc aaagcagcgg
     1981 caattcgtta gtgcaggata tcggatcggc gttagccaac aaaccgcctg catcggacgc
     2041 cgagatccag ttgattcgtt cgcgcctggt gcttggtaaa acggtggatg atctcgacct
     2101 cgatattgcg gtgagcaaaa acacgttccc gattttcggt gcgggctggg atcgcctgat
     2161 gggacgccag aacgagacgg tgaaagtgac tacctttaac cgtccgaaag agatggagga
     2221 tcaggtgttt acgcttaatg tgctggacaa caaaaactac accctgagca gcgatggcgg
     2281 ctttagcgcc cgtgggcaag cgggccagat actgaaaaaa gaaggcgtca cgctgatggt
     2341 tgaagccatt cacgcccgcc cgggcagtga gtttaccgtc accaaatact ccacgctggg
     2401 gatgatcaat caactgcaaa acagcctgac ggtaacggag aacggcaaag acgcaggcgt
     2461 actgagcctg acttataccg gtgaagatcg cgaacagatc cgcgacattc ttaacagcat
     2521 cgcccgtaac tatcaggaac aaaatattga gcgcaaatcg gcggaagcgt cgaaaagcct
     2581 cgctttcctc gcgcaacagt taccggaagt acgtagccgc cttgatgttg ccgaaaacaa
     2641 actgaatgcc ttccgtcagg ataaagattc tgttgatctg ccgctggaag cgaaagcggt
     2701 gctcgattcg atggtgaaca tcgacgccca gttgaacgaa ctgaccttta aagaggcgga
     2761 aatctccaag ctgtacacca aagttcaccc cgcgtaccgc acgctgctgg agaaacgtca
     2821 ggcgctggaa gacgaaaaag ccaaacttaa tggtcgcgta acggcgatgc cgaaaaccca
     2881 gcaggaaatt gtccgtctga cccgcgatgt cgagtctggt cagcaggtct atatgcaact
     2941 gctgaataaa gagcaggagc tgaaaatcac cgaggccagc accgtcggcg atgtgcgcat
     3001 tgttgacccg gcaatcactc agcctggtgt gctaaaaccg aagaaagggc tgattatcct
     3061 tggggcgatt atccttggcc tgatgctctc tatcgtgggg gtgctgctgc gctcgttgtt
     3121 taatcgcggc atcgaaagcc cgcaggtgct ggaagaacac ggtatcagcg tctatgccag
     3181 catcccgctg tcggagtggc agaaagcgcg cgatagcgtc aaaaccatca aagggattaa
     3241 acgctataaa cagagccagc tactggcggt ggggaatcca accgatctgg cgattgaagc
     3301 catccgcagc cttcgtacca gtttgcactt cgcgatgatg caggcgcaga acaatgtgtt
     3361 gatgatgacc ggggttagcc cgtcaatcgg taaaaccttt gtctgcgcca acctggcggc
     3421 ggtgatcagc cagaccaata aacgcgtgtt gttgatcgac tgcgatatgc gcaaaggcta
     3481 cacccatgag ctgttgggca ctaataacgt taatggcctg tcggaaattc tgattggtca
     3541 gggcgatatt actacagctg ctaaaccgac ctctattgcc aaatttgacc tgatcccgcg
     3601 cggtcaggta ccgccaaatc cttctgaact gttgatgagc gaacgctttg ccgaactggt
     3661 gaactgggcg agtaaaaact acgacctggt gttgattgat acgccgccga ttctggcagt
     3721 gaccgatgcg gcaattgttg gtcgtcatgt cggaaccacg ttaatggtgg cgcgttatgc
     3781 ggtcaacaca ttgaaagaag tggaaaccag tctgagccgc tttgagcaaa acggtattcc
     3841 ggtgaaaggg gtgattctga actccatctt ccgccgcgcc agcgcgtatc aggattatgg
     3901 ctattacgaa tacgaatata agtcggatgc gaaataacga ggcctgcatt cgcaccgccc
     3961 cgtaggccgg ataaggcgct cacgccgcat ccggcaagca aaccagctca taagccggga
     4021 gtacaaccta tgaaagacaa tccgctgatc tcaatctata tgccgacctg gaaccgccaa
     4081 caactggcga ttcgggcgat aaaatcggtc ttgcgccagg actacagcaa ctgggagatg
     4141 atcatcgtgg atgattgctc cacctcctgg gaacaactgc aacagtacgt caccgccctc
     4201 aacgatccgc gtattaccta cattcacaac gacattaaca gcggggcgtg cacggtacgt
     4261 aaccaggcga ttatgctggc gcaaggggaa tacattaccg ggatcgatga cgatgatgaa
     4321 tggacgccca accgcctgag cgtcttcctc gcccataaac agcaactggt cactcacgcc
     4381 tttttgtacg ctaacgatta cgtttgccag ggcgaagttt attcccaacc ggcaagctta
     4441 ccactgtatc ccaaatcgcc gtactcacgc cgcctgttct ataaacgcaa tatcattggc
     4501 aatcaggtct ttacctgggc atggcgtttc aaagagtgtt tgttcgatac cgaactgaaa
     4561 gcggcacagg attacgacat cttcctgcgg atggtggtgg agtatggcga accgtggaaa
     4621 gtggaagagg cgacgcagat cctgcatatc aatcacggtg aaatgcagat cacctcgtca
     4681 ccgaaaaaat tctccggata cttccacttt taccgcaagc acaaagacaa attcgaccgt
     4741 gccagtaaaa aatatcagct gtttaccctc taccagatcc gcaataaacg tatgacctgg
     4801 cgcactttgc tgacgctgct gtcggtgcgt aacggcaaac gtctggctga tggtattcgg
     4861 gggcgctaaa tatgctggaa gatctacgcg ccaacagctg gagtttacgc ccgtgctgca
     4921 tggttcttgc ctatcgcgtc gctcattttt gttcggtgtg gcgcaaaaag aacgttctca
     4981 acaatctttg ggcggccccg ctgctggtgc tgtatcgcat tatcaccgaa tgctttttcg
     5041 gttatgaaat ccaggctgcc gcgaccattg gccgccgctt tactatccat cacggttacg
     5101 ccgtagtgat caataaaaac gtggtagcgg gggatgattt caccattcgc cacggcgtca
     5161 ctatcggcaa tcgtggtgcc gataacatgg catgtccaca cattggcaac ggcgtcgaac
     5221 tcggtgccaa cgtcattatt cttggtgata tcacgcttgg taacaacgtc accgtgggcg
     5281 cgggcagcgt ggtgctcgac tctgtcccgg acaacgcgct ggtggtggga gaaaaagcgc
     5341 gagtgaaggt aattaaatga atattctgca atttaatgtg cgactggcgg aaggcggggc
     5401 ggcaggtgtg gcgttagatc tccaccagcg tgcgctgcaa caggggctgg cgtcacattt
     5461 tgtctacggc tacggcaaag gcggcaaaga gagcgtcagc catcagaact atccgcaggt
     5521 catcaaacat acgccgcgga tgaccgcaat ggcgaatatt gccctgtttc gtctgtttaa
     5581 tcgcgatctg tttggcaatt tcaatgagtt atatcgcacc attactcgta caccgggtcc
     5641 ggtggtcctg cattttcatg tgctgcacag ctactggcta aatcttaagg gcgtggtgcg
     5701 cttttgcgaa aaggtgaaaa accacaaacc ggatgtcact ctggtctgga cgctgcacga
     5761 ccactggagc gttaccgggc gctgcgcctt taccgacggt tgtgaaggct ggaaaacggg
     5821 ctgccagaaa tgcccgacct taaataacta tccgccggtg aagattgatc gggcgcacca
     5881 gctggtggcg ggcaaacgcc agttattccg tgagatgctg gcgctgggct gtcagtttat
     5941 ttcccccagc cagcatgtgg ctgacgcttt caatagcctg tacggtccag ggcgttgccg
     6001 gattatcaat aacggtattg atatggcaac cgaggcgatt ctggcggatt tgcctccggt
     6061 gcgcgaaact cagggcaagc cgaaaatcgc ggtggtagcg catgacctgc gttacgacgg
     6121 caaaactaac cagcaactgg tacgcgagat gatggcgctg ggcgacaaaa tcgaactgca
     6181 tacctttggt aagttctcgc cgttcaccgc tggcaacgtg gttaatcacg gctttgaaac
     6241 tgacaagcgc aagttgatga gcgcgctcaa tcagatggat gcgctggtgt tcagttctcg
     6301 cgtcgataac tacccgctga ttttgtgtga ggcgttatca attggcgtgc cggtgattgc
     6361 cacccatagc gacgcggcgc gtgaagtgtt gcaaaaatcc ggcggtaaaa ccgtcagcga
     6421 agaagaggtg ctgcaactgg tccagttaag caaaccggaa atcgcgcagg cgttatttgg
     6481 taccacgctg gctgagttca gccaacgcag ccgcgtcgcc tacagtggac aacagatgct
     6541 ggaggagtat gtcaacttct atcagaatct gtagctacct gctgctgccg ctgatctacc
     6601 tgctggttaa cgtcaaaatc acccagcttg gcgaaagttt ccccatcacc atcgtcactt
     6661 ttttacctgt cttgttactg ctgtttttag aacgcatcag cgttaaaaaa ttgatgattg
     6721 ccttagggat ttgcgcggga ctcacggcgt ttaactacct gtttggtcag tcgctggatg
     6781 ccagtaaata cgtcacttca actatgctgt ttgtctatat cgtgatcatt attggtatgg
     6841 tgtggagtat tcgctttaaa acgatttcgc cacacaacca tcgcaagata ttacgtttct
     6901 tttatctggt ggtcgggctg gtggtggcgc tggcggcggt ggagatggcg caaattatcc
     6961 tcaccggcgg cagcagtatt atggagtcga tttcgaaata tctgatttac agcaacagct
     7021 atgtgctgaa tttcatcaaa ttcggcggca agcgcacgac agcactttat ttcgaaccgg
     7081 catttttcgc tctggcatta atctcaattt ggctcagcat caaacagttt ggtatcaaaa
     7141 cgcctaaaac agatgctatg attctcgcag ggataatatt atccggatcg ttttcagggg
     7201 ttatgacctt tatcctgttt tatttgctgg agtgggcatt tcaatatctg aataaagagg
     7261 cgattaagaa aaagttaccg ttagcattga tttctctggc agtattcctg gttggtgtgg
     7321 taattgcatt tccttatatt tccacccgtc tgggcgattt aggtacggaa ggatcgtcat
     7381 cgtattatcg tattgtcggt ccgctggtga tggtcggtta ttctttgacc catattgacg
     7441 gtgtagtcag atttggctca ctttatgaat atgtcgcatc attcggaata tttaacggtg
     7501 cggatgtcgg aaaaaccata gacaatggtt tgtatctgct gattatttat ttttcttggt
     7561 tcgcggtgtt tttatcgctg tggtacatgg ggaaagtgat aaaaatgatg atcaacgctt
     7621 ttggtgataa ccgcaatttt cgcgtgcaat tatatctttt tactccggta tcgctgtttt
     7681 ttaccggttc gatatttagc ccggaatatg catttttaat cgtctgtccg tttattttgc
     7741 gaaaagcgtt aaatattacg aggtaagaat aagaacatgt tgcttagcat aatcactgtc
     7801 gcgtttcgta acctcgaagg gatagtcaaa acacatgcct cgctggcgca tctggcgcag
     7861 gcggaagata tcagcttcga atggattgtt gtcgatggcg gttccaatga cggcactcgt
     7921 gagtacctgg aaaatctcaa tggtatctat aacctacgct ttgtcagcga gccagataac
     7981 ggtatctacg acgccatgaa caagggcatt gcgatggcac aaggcaagtt cgcgttgttt
     8041 ctcaactcgg gcgatatttt tcatcaggat gccgcatatt ttgtccgtaa gttaaaaatg
     8101 caaaaagata acgtgatgat caccggcgat gcgctgctgg attttggcga cgggcataaa
     8161 attaaacgta gcgccaaacc gggctggtat atttatcaca gcctgcccgc cagtcatcag
     8221 gcgatatttt tcccggtatc cggtttgaaa aaatggcgtt atgacctgga atataaagtt
     8281 tcttctgatt acgcgctggc agccaaaatg tataaagcag gttatgcatt taaaaaactc
     8341 aatggcctgg tgtctgaatt ttctatgggt ggggtatcta ccaccaataa tatggaattg
     8401 tgtgctgacg cgaaaaaagt ccaacggcaa atattacatg tgcctggctt ttgggttgaa
     8461 ttatcctggc atttacgcca gcgtactacc tcaaagacga aagccttata taacaaaagc
     8521 tgaatataag ggaaaacgat gcaggattta agcggtttct cggtgccgaa agggttccgg
     8581 ggcggcaacg ctattaaagt gcaattgtgg tgggcagtac aggcaacaat atttgcctgg
     8641 tcgccacaag tattgtatcg ctggcgggca tttttattac gtttattcgg cgcaaaaata
     8701 ggaaaaaacg tagttattcg tccgtcagta aaaattacct atccgtggaa attaacctta
     8761 ggtgattacg tgtgggtcgg cgatgacgtc aatttatata ccctcggtga aataaccatt
     8821 ggcgcacatt cggtgatatc gcaaaaaagt tatttatgca ccgggagcca cgaccatgca
     8881 agtcaacatt tcaccattaa cgccacgcct attgtgattg gcgagaaatg ctggctggca
     8941 accgatgttt ttgttgcccc aggcgtcacg attggcgacg gcaccgtcgt gggtgcgcga
     9001 agcagtgttt ttaaatcgct tccggcaaat gtggtttgcc gggggaatcc cgcagtggtg
     9061 atacgcaaac gcgttgaaac tgaataaatt caaaaaaaat acagaggaat aagacatgtc
     9121 aaaagtcgct ctcatcaccg gtgtaaccgg acaagacggt tcttacctgg cagagtttct
     9181 gctggaaaaa ggttacgagg tgcatggtat taagcgtcgc gcatcgtcat tcaacaccga
     9241 gcgcgtggat cacatttatc aggatccgca cacctgcaac ccgaaattcc atctgcatta
     9301 tggcgacctg agtgatacct ccaacctgac gcgtattttg cgtgaagtgc agccggatga
     9361 agtgtacaac ctgggcgcaa tgagccacgt tgcggtttct tttgagtcac cggaatatac
     9421 cgctgacgtc gacgcgatgg gtacgctgcg cctgctggag gcgatccgct tcctcggtct
     9481 ggaaaagaaa acccgtttct atcaggcttc cacctctgaa ctgtatggtc tggtgcagga
     9541 aattccgcag aaagagacca cgccgttcta tccgcgctct ccgtatgcgg tcgccaaact
     9601 gtatgcctac tggatcaccg tcaactaccg tgaatcctac ggcatgtatg cctgtaacgg
     9661 tattctcttc aaccatgaat ccccgcgccg cggcgaaacc ttcgttaccc gcaaaatcac
     9721 ccgcgcaatc gccaacatcg cccaggggct ggagtcgtgc ctgtacctcg gcaatatgga
     9781 ttccctgcgt gactggggcc atgccaaaga ctacgtaaaa atgcagtgga tgatgctgca
     9841 acaggaacag ccagaagatt tcgttatcgc gaccggcgtt cagtactccg tacgtcagtt
     9901 cgtagaaatg gcggcagcac agctgggcat caaactgcgc tttgaaggca cgggcgttga
     9961 agagaagggc attgtggttt ccgtcaccgg gcatgacgcg ccgggtgtta aaccgggtga
    10021 tgtgattatc gccgttgatc cgcgttactt ccgtccggct gaagttgaaa cgctgctcgg
    10081 cgacccgacc aaagcgcacg aaaaactggg ctggaaaccg gaaatcaccc tcagagagat
    10141 ggtgtctgaa atggtggcta atgacctcga agcggcgaaa aaacactctc tgctgaaatc
    10201 tcacggctac gacgtggcga tcgcgctgga gtcataagta tgagtagaca acgcattttt
    10261 attgctggcc atcgcgggat ggtcggttcc gccatcaggc ggcagctcga acagcgcggt
    10321 gatgtggaac tggtattacg cacccgcgac gagctgaacc tgctggacag ccgcgccgtg
    10381 catgatttct ttgccagcga acgtattgac caggtctatc tggcggccgc gaaagtgggc
    10441 ggcattgttg ccaacaacac gtatccggcg gatttcatct atcagaacat gatgattgag
    10501 agcaacatca ttcacgccgc gcatcagaac gacgtgaaca aactgctgtt tctcggatcg
    10561 tcctgcatct acccgaaact ggcaaaacag ccgatggcag aaagcgagtt attgcagggc
    10621 acgctggagc cgaccaacga gccttacgcc attgccaaaa tcgccgggat caaactgtgc
    10681 gaatcataca accgccagta cggacgcgat taccgctcag tcatgccgac caacctgtat
    10741 ggcccgcatg acaacttcca cccgagtaat tcgcatgtga tccctgcatt gctgcgtcgc
    10801 ttccacgagg cgacggcaca gaatgcaccg gacgtggtgg tgtggggcag cggtacgccg
    10861 atgcgagaat ttctgcacgt cgatgatatg gcggcggcga gcattcatgt catggagctg
    10921 gcgcatgaag tctggctgga gaacacccag ccgatgctgt cgcacattaa cgtcggcacg
    10981 ggcgttgact gcactatccg cgagctggcg caaaccatcg ccaaagtggt gggttacaaa
    11041 ggtcgggtgg tttttgatgc cagcaaaccg gatggtacgc cgcgcaaact gctggatgtg
    11101 acgcgcctgc atcagcttgg ctggtatcac gaaatctcac tggaagcggg gcttgccagc
    11161 acttaccagt ggttccttga gaatcaagac cgctttcggg ggtaatgatg tttttacgtc
    11221 aggaagactt tgccacggta gtgcgctcca caccgcttgt ctctctcgac tttattgtcg
    11281 agaacagtcg cggcgagttt ctgcttggca aaagaaccaa ccgcccggcg cagggttact
    11341 ggtttgtgcc gggagggcgc gtgcagaaag acgaaacgct ggaagccgca tttgagcggc
    11401 tgacgatggc ggaactgggg ctgcgtctgc cgataacagc aggccagttt tacggtgtct
    11461 ggcagcactt ttatgacgat aacttctccg gcacggattt caccactcac tatgtggtgc
    11521 tcggttttcg cttcagagta gcggaagaag aactgttact gccggatgag cagcatgacg
    11581 attaccgctg gctgacgccg gacgcgctgc tcgccagtaa cgatgttcac gctaacagcc
    11641 gcgcctattt tctcgctgag aagcgtgccg gagtacccgg attatgaaaa tactggtcta
    11701 cggcattaac tactcgccgg agttaaccgg catcggcaaa tacaccggcg agatggtgga
    11761 atggctggcg gcgcaaggtc atgaggtgcg ggttattacc gcaccgcctt actacccgca
    11821 gtggcaagtg ggcgagaact attccgcctg gcggtacaaa cgagaagagg gggccgctac
    11881 ggtgtggcgc tgcccgctgt acgtgccaaa acagccgagc accctgaaac gcctgttgca
    11941 tctcggcagt tttgccgtca gcagtttttt cccgttgatg gcgcaacgtc gctggaagcc
    12001 ggatcgcatt atcggcgtag tgccaacgct gttttgcacg ccgggaatgc gcctgctggc
    12061 gaaactctct ggcgcgcgta ccgtgctgca tattcaggat tacgaagtag atgccatgct
    12121 ggggctgggc cttgccggaa aaggcaaagg cggcaaagtg gcacagctgg caacggcgtt
    12181 cgaacgtagc ggactgcata acgtcgataa cgtttccacg atttcgcgtt cgatgatgaa
    12241 taaagccatc gaaaaaggcg tggcggcgga aaacgtcatc ttcttcccca actggtcgga
    12301 aatcgcccgt tttcagcatg ttgcagacgc cgatgttgat gcccttcgta accagcttgg
    12361 cctgccggat aacaaaaaaa tcattcttta ctccggcaat attggtgaaa agcaggggct
    12421 ggaaaacgtt attgaagcag ccgatcgcct gcgcgatgaa ccgctgattt ttgccattgt
    12481 cgggcagggc ggcggcaaag cgcggctgga aaaaatggcg cagcagcgtg gactgcgcaa
    12541 catgcaattt ttcccgctgc aatcgtatga cgctttaccc gcactgctga agatgggcga
    12601 ttgccatctg gtggtgcaaa aacgcggcgc ggcagatgcc gtattgccgt cgaaactgac
    12661 caatattctg gcagtaggcg gtaacgcggt gattactgct gaagcccaca cagaactggg
    12721 acagctttgc gaaacctttc cgggcattgc ggtttgcgta gaaccggaat cggttgaggc
    12781 gctggtggcg gggattcgtc aggcgctcct gctgcccaaa cacaacacgg tggcacgtga
    12841 atatgccgaa cgcacgcttg ataaagagaa cgtgttacgt caatttataa atgatattcg
    12901 gggataatta tggcgcagtc gaaactctat ccagttgtga tggcaggtgg ctccggtagc
    12961 cgcttatggc cgctttcccg cgtactttat cccaagcagt ttttatgcct gaaaggcgat
    13021 ctcaccatgc tgcaaaccac catctgccgc ctgaacggcg tggagtgcga aagccctgtg
    13081 gtgatttgca atgagcagca ccgctttatt gttgcggaac aactgcgtca actgaacaaa
    13141 ctcactgaga acattattct cgaaccggca gggcgtaaca cagcaccggc cattgcgctg
    13201 gcggcgctgg cggcaaaacg tcatagcccg gagaacgacc cattaatgct ggtgctggcg
    13261 gcggatcatg tgattgccga tgaagacgca ttccgcgccg ccgtgcgtaa cgctatgccg
    13321 tatgccgaag cgggcaagct ggtgaccttc ggcattgtgc cggatctacc tgaaaccgga
    13381 tatggctata ttcgtcgcgg tgaagtgtcg gcgggtgagc aggatacggt ggcctttgaa
    13441 gtggcgcagt ttgtcgaaaa accaaatctg gaaaccgcgc aggcctatgt ggcaagcggc
    13501 gaatattact ggaacagcgg tatgttcctg ttccgtgccg gacgctatct cgaagaactg
    13561 aaaaaatatc gcccggatat tctcgacgcc tgtgaaaaag cgatgagcgc cgtcgatccg
    13621 gatctcgatt ttattcgcgt ggatgaagac gcgtttctgg cctgcccgga agagtcggtg
    13681 gattacgcgg tcatggaacg tacggcagat gccgttgtgg tgccgatgga tgcgggctgg
    13741 agcgatgtcg gctcctggtc ctcattatgg gagatcagcg cccacaccgc cgagggcaac
    13801 gtttgccacg gcgatgtgat taatcacaaa actgaaaaca gctatgtgta tgccgaatct
    13861 ggcctggtca ccaccgtcgg ggtgaaagat ttggtggtag tgcagaccaa agatgcggtg
    13921 ctgattgccg accgtaacgc ggtgcaggat gtgaaaaaag tggtcgagca gatcaaagcc
    13981 gatggtcgcc atgagcatcg ggtgcatcgc gaagtctatc gcccgtgggg caaatatgac
    14041 tctatcgacg cgggcgaccg ctaccaggtg aaacgcatca ccgtgaaacc gggcgaaggc
    14101 ttgtcggtac agatgcacca tcaccgcgcg gaacactggg tggttgtcgc gggaacggca
    14161 aaagtcacca ttgacggtga tatcaaactg cttggtgaaa acgagtcgat ttatattccg
    14221 ctgggggcga cgcactgcct ggaaaacccg gggaaaattc cgctcgattt aattgaagtg
    14281 cgctccggct cttatctcga agaggatgat gtggtgcgct tcgcggatcg ctacggacgg
    14341 gtgtaaacgt cgcatcaggc gctgattgtc ggatgcggcg tgaacgcctt atccgaccta
    14401 cggttcggtt ttgtaggcct gataagacgc ggcagcgtcg catcaggcaa tgaatgcgaa
    14461 accgcggtgt aaataacgac aaaaacaaaa ttggccgttt cggtcagggc caactattgc
    14521 ctgaaaaagg gtaacgatat gaaaaaatta acctgcttta aagcctatga tattcgcggg
    14581 aaattaggcg aagaactgaa tgaagatatt gcctggcgca ttggtcgtgc ctatggcgaa
    14641 tttctcaaac cgaaaaccat tgtgttaggc ggtgatgccc gtctcaccag cgaaacctta
    14701 aaactggcgc tggcaaaagg tttacaggat gcgggcgttg acgtgctgga tattggtatg
    14761 tccggcaccg aagagatcta tttcgccacg ttccatctcg gcgtggatgg cggcattgaa
    14821 gttactgcca gccataaccc gatggattac aacggcatga aactggtgcg cgaaggggct
    14881 cgcccgatca gcggcgatac cggactgcgc gatgtccagc gtctggcaga agccaacgac
    14941 tttcctcccg ttgatgaaac caaacgcggt cgctatcagc aaatcaatct gcgtgacgct
    15001 tacgttgatc acctgttcgg ttatatcaac gtcaaaaacc ttacgccgct caagctggtg
    15061 atcaactccg ggaacggcgc ggcgggtccg gtggtggacg ccatcgaagc ccgctttaaa
    15121 gccctcggcg caccggtgga attgatcaaa gtgcacaaca cgcccgacgg caatttcccc
    15181 aacggtattc ctaacccgct gctgccggaa tgtcgcgacg acacccgcaa tgcggtcatt
    15241 aaacacggcg cggatatggg cattgctttt gatggcgatt ttgaccgctg tttcctgttt
    15301 gacgaaaaag ggcagtttat cgagggctac tacattgtcg gtctgctggc agaagcgttc
    15361 ctcgaaaaaa atcccggcgc gaagatcatc cacgatccac gtctctcctg gaataccgtt
    15421 gatgtggtga ctgccgcagg cggcaccccg gtaatgtcga aaaccggaca cgcctttatt
    15481 aaagaacgga tgcgcaagga agacgccatc tacggtggcg aaatgagcgc ccaccattac
    15541 ttccgtgatt ttgcttactg cgacagcggc atgatcccgt ggctgctggt cgccgaactg
    15601 gtgtgcctga aaggaaagac gctgggcgaa ctggtgcgcg accggatggc agcgtttccg
    15661 tcaagcggtg agatcaacag caaactggcg caacccgttg aggcgattaa ccgcgtggaa
    15721 cagcatttta gccgtgaggc gctggcggtg gatcgcaccg atggcatcag catgaccttt
    15781 gccgactggc gctttaatct gcgctcctcc aacaccgaac cggtggtgcg gttgaatgtg
    15841 gaatcacgcg gtgatgtgcc gctgatggaa gcgcgaacgc gaactctgct gacgttgctg
    15901 aacgagtaat gtcggatctt cccttacccc attgcgggta aggggctaat aacaggaaca
    15961 acgatgacaa atctaaaaaa gcgcgagcga gcgaaaacca atgcatcgtt aatctctatg
    16021 gtgcaacgct tttcagatat caccatcatg tttgccggac tatggctggt ttgcgaagtg
    16081 agcggactgt cattcctcta catgcacctg ttggtggcgc tgattacgct ggtggtgttc
    16141 cagatgctgg gcggcatcac cgatttttat cgctcatggc gcggtgttcg ggcagcgaca
    16201 gaatttgccc tgttgctaca aaactggacc ttaagcgtga ttttcagcgc cggactggtg
    16261 gcgttcaaca atgatttcga cacgcaactg aaaatctggc tggcgtggta tgggctgacc
    16321 agtatcggac tggtggtttg ccgttcatgt attcgcattg gggcgggctg gctgcgtaat
    16381 catggctata acaagcgcat ggtcgcggtg gcgggggatt tagccgccgg gcaaatgctg
    16441 atggagagct tccgtaacca gccgtggtta gggtttgaag tggtgggcgt ttaccacgac
    16501 ccgaaactgg gcggcgtttc taacgactgg gcgggtaacc tgcaacagct ggtcgaggat
    16561 gctaaagcag gcaagattca taacgtctat atcgcgatgc aaatgtgcga cggcgcgcga
    16621 gtgaaaaaac tggtccatca actggcggac accacctgtt cggtgctgct gatccccgat
    16681 gtctttacct tcaacattct ccattcacgt cttgaagaga tgaacggcgt tccggtggtg
    16741 ccgctttacg acacgccgct ttccggggtt aaccgcctgc tcaaacgtgc ggaagacatt
    16801 gtgctggcga cgcttatcct gctgctgatc tccccggtgc tgtgctgcat tgcgctggcg
    16861 gtgaaactca gttcacctgg gccggttatt ttccgccaga ctcgctacgg catggatggc
    16921 aagccgatca aagtgtggaa gttccgttcc atgaaagtga tggagaacga caaagtggtg
    16981 acccaggcga cgcagaacga tccgcgcgtc accaaagtgg ggaactttct gcgccgcacc
    17041 tcgctggatg aattgccgca gtttatcaat gtgctgaccg gggggatgtc gattgtcggt
    17101 ccacgtccgc acgcggtggc gcataacgaa cagtatcgac agctcattga aggctacatg
    17161 ctgcgtcata aggtgaaacc gggcattacc ggctgggcgc agattaacgg ctggcgcggc
    17221 gaaaccgaca cgctggagaa aatggaaaaa cgcgtcgagt tcgaccttga gtacatccgc
    17281 gaatggagcg tctggttcga tatcaaaatc gttttcctga cggtattcaa aggattcgtt
    17341 aacaaagcgg catattgata tgagcttacg tgaaaaaacc atcagcggcg cgaagtggtc
    17401 ggcgattgcc acggtgatca tcatcggcct cgggctggtg cagatgaccg tgctggcgcg
    17461 gattatcgac aaccaccagt tcggtttgct taccgtgtcg ctggtgatca tcgcgctggc
    17521 agatacgctt tctgacttcg gtattgctaa ctcgattatt cagcgaaaag aaatcagcca
    17581 ccttgaactc accacgctgt actggctgaa cgttgggctg gggcttgtgg tgtgtgtggc
    17641 ggtgtttttg ttgagtgatc tcattggtga cgtgctgaac aacccggacc tggcaccgtt
    17701 gattaaaaca ttgtcgctgg cgtttgtggt tatcccccac gggcaacagt tccgcgcgtt
    17761 gatgcaaaag gagctggagt tcaacaaaat cggcatgatc gaaaccagcg cggtactggc
    17821 gggcttcact tttacggtgg ttagcgccca tttctggccg ctggcgatga ccgcgatcct
    17881 cggttatctg gtcaatagtg cggtgagaac gctgctgttt ggctactttg gccgcaaaat
    17941 ttatcgcccc ggtctgcatt tctcgctggc gtctgttgcg ccgaacttac gctttggtgc
    18001 ctggctgacg gcggacagca tcatcaacta tctcaatacc aacctttcaa cgctggtgct
    18061 ggcgcgtatt ctcggcgcgg gcgtggcagg gggatacaac ctggcgtaca acgtggccgt
    18121 tgtgccgccg atgaagctga acccgatcat cacccgtgtg ttgtttccgg cattcgccaa
    18181 aattcaggac gataccgaaa agctgcgcgt caacttctac aagctgctgt cggtggtggg
    18241 gattatcaac tttccggcgc tgctcgggct gatggtggtg tcaaataact ttgtaccgct
    18301 ggtatttggc gaaaagtgga acagcattat tccggtgctg caattgctgt gtgtggtggg
    18361 tctgctgcgc tccgtaggta acccgattgg ttcgctgctg atggcgaaag cgcgggtcga
    18421 tatcagcttt aaattcaacg tattcaaaac atttctgttt attccggcga ttgttattgg
    18481 tgggcagatg gcgggcgcaa tcggcgtcac gctcggtttc ctgctggtgc agattatcaa
    18541 caccattctg agttacttcg tgatgattaa accggttctt ggttccagtt atcgccagta
    18601 catcctgagt ttgtggctgc cgttttatct ctcgctgccg acgctggtgg tcagttatgt
    18661 gctgggcatt gtgctgaaag ggcaactggc gctggggatg ctgctggcgg tgcaaatagc
    18721 cgcgggcgtg ctggcgtttg tggtgatgat tgtgctgtcg cgccatccgc tggtggtgga
    18781 agtgaagcgc cagttttgtc gcagcgaaaa aatgaaaatg cttttacggg cggggtgagt
    18841 tgttgttttc ggttccgaca acggttgccg ggtgcagcgt aaaacacctt atccatccta
    18901 cggttctgtg ctcgggtctt gtaggcctga taagacgcgc cagcgtcgca tcaggcaaaa
    18961 aacggcaacg aacacaacaa acacagcctc acctggcatc aaatttatga ggaaaaaatg
    19021 aaattactta ttctgggcaa ccacacttgc ggcaatcgtg gcgacagcgc catcttgcgc
    19081 ggcttacttg atgccatcaa catcctcaat ccacataccg aagtggacgt gatgagccgc
    19141 tatccggtca gttcttcctg gctgctcaac cgcccggtaa tgggtgatcc gctgttcctt
    19201 caaatgaaac aacacaacag cgcggcgggc gttgtcgggc gcgttaaaaa agtcctccgt
    19261 cgccgctatc agcaccaggt attgctctca cgcgtcaccg acactggcaa gctgcgcaat
    19321 atcgccatcg cccagggatt caccgacttc gtgcgcctac tgtcaggtta cgacgccatt
    19381 attcaggtcg gcggatcgtt ttttgtcgat ctctacggcg tgccgcagtt tgaacatgca
    19441 ctttgcacat ttatggcgaa aaagccgctg tttatgattg gtcacagcgt cggtcccttc
    19501 caggatgagc aatttaacca actggcgaac tacgtttttg gtcactgcga cgcgctgatc
    19561 ttgcgcgaat cggtcagcct cgatctaatg aaacgcagca atatcaccac tgcaaaagtt
    19621 gaacatggcg tcgataccgc gtggctggtc gatcaccaca cagaagactt caccgccagc
    19681 tatgccgtcc aacactggct ggacgttgcc gcacaacaga aaacggtggc aattaccctg
    19741 cgcgaactgg caccgttcga caaacgcctc ggcaccactc agcaagtgta tgaaaaagcc
    19801 tttgccgggg tggtcaatcg cattctcgac gaaggctatc aggtcattgc gctctccacc
    19861 tgtacgggta tcgacagcta taacaaagac gaccgcatgg tggcgctcaa cctgcgccag
    19921 cacatcagcg atcctgcccg ttatcacgta gtgatggatg aacttaacga tctggaaatg
    19981 ggcaaaattc tcggtgcctg tgaactcacc gtcggtacgc gcctgcactc cgccattatc
    20041 tcaatgaact ttgctactcc ggcgattgcc atcaactatg aacataaatc cgccggggtt
    20101 atgcagcagc tgggactacc ggagatggca attgatatcc gtcatttatt agacggcagc
    20161 ctgcaagcga tggttgcgga taccttaggc cagcttccgg cgctgaacgc acgacttagc
    20221 gaagccgtca gtcgtgagcg tcagacggga atgcagatgg tgcagtccgt actggaacgc
    20281 atcggggagg tgaaatgaag gtcggcttct tcttattgaa atttccgctg tcgtcagaaa
    20341 ccttcgttct taaccaaatc actgcgttta ttgatatggg ctttgaagtg gagattgtcg
    20401 cgctgcaaaa aggcgacaca caaaacaccc acgtggcatg gactaaatac aaccttgccg
    20461 ccagaacccg ctggttacag gacgaaccta cgggcaaagt ggcgaaactg agccaccgag
    20521 ccagtcagac cttgcgcggc attcatcgta aaaatacctg gcaggcgctc aacctcaaac
    20581 gctatggtgc cgagtcgtgg aacctgattt tgtctgccat ttgcggccag gtcgcaacac
    20641 cgtttcgcgc cgatgtgttc atcgctcatt ttggccctgc gggggtaacc gcagcaaaac
    20701 tacgcgaact gggtgtcatt cacggcaaaa ttgccactat tttccacggc attgatattt
    20761 ccagtcggga agtgctcaac cattacactc ccgaatatca acaactgttt cgacgtggcg
    20821 acctgatgct accgataagc gatctgtggg ccggaaggct gcaaaaaatg ggctgcccga
    20881 gggaaaaaat cgccgtatcg cgcatgggcg tagatatgac gcgctttagc ccgcgtcccg
    20941 tgaaagcgcc cgcaacaccg ctggagatta tttccgtcgc acgcttaacc gagaaaaaag
    21001 gtctgcatgt ggcgattgaa gcctgccggc agttgaaaga gcagggcgtg gcatttcgct
    21061 atcgcatcct cggcattggc ccgtgggaac gacgcctgcg cacgctcatc gaacaatatc
    21121 aactggaaga tgtggtggag atgccgggct ttaaaccgag ccatgaagtg aaagcgatgc
    21181 tcgacgacgc ggatgtcttc ctgttgccat cggttacagg tgcggatggc gatatggaag
    21241 gcattccggt ggcgctgatg gaggcgatgg cggtcggcat tccggtggtt tctactctgc
    21301 atagcggaat accggaactg gtggaggctg acaaatccgg ctggctggtg cctgagaacg
    21361 atgctcgcgc actggcgcaa cgactggcgg cgtttagcca actggacacc gacgaattgg
    21421 ctccggtcgt caaacgcgcg cgcgaaaaag ttgaacacga ttttaaccag caggtgatca
    21481 atcgagaact cgccagcttg ctgcaggctt tatagaggag aacgtatgcc atttaaaaaa
    21541 ctctcccgac gcaccttcct gacggcaagc tcggcgcttg ccttccttca tacccctttc
    21601 gctcgcgcat ttcccgcccg acaaagcgtt aatatcaacg actacaaccc tcacgactgg
    21661 atcgcctcat ttaaacaagc cttcagcgaa gggcaaacgg ttgtcgtgcc tgctggattc
    21721 gtttgtgaca atatcaacac cggcatcttc attcctcctg gcaaaacgtt acacatcctt
    21781 ggaagcctgc gcggcaacgg cagagggcga tttgtcttac aggacggcag ccaggtgaca
    21841 ggggaggagg gcggcagtat gcataacatc accctggatg tgcgtggttc tgactgcacc
    21901 atcaaagggc tggcgatgag cggctttggt ccggtaacgc agatttatat cggcggcaaa
    21961 aacaaacggg tcatgcgcaa cctgaccatc gataacctga ccattagcca cgctaattac
    22021 gccatcttac gccagggatt tcataaccag attatcggtg ccaacatcac caattgtaag
    22081 ttcagcgact tacagggcga tgccattgaa tggaacgtgg cgattaacga ccgcgatatt
    22141 ttgatctccg accatgtcat cgagcgcatc aactgtacta acggcaaaat caactggggc
    22201 attggcatag gccttgcggg aagcacttac gataataatt acccggaaga tcaggcagtg
    22261 aaaaactttg tcgtggcgaa tatcacggga tcggattgtc ggcagttgat acatgttgaa
    22321 aatggtaaac attttgttat tcgtaatatc aaagcccgca atatcacgca ggatttcagt
    22381 aagaaagcag gtattgataa cgccacagtc gctatttacg gttgtgacaa tttcgtgatt
    22441 gataatattg aaatgattaa tagcgccggg atgttaatcg gctatggggt aattaaaggc
    22501 aattatctct cgattccgca aaatttccga gtgaataata ttcaactgga taacacccat
    22561 cttgcttata aattgcgcgg cctccaaatc tctgccggga atgccgtctc ctttgtggca
    22621 ctaactaaca ttgagatgaa gcgtgcgtcg ctggagttac acaacaaacc gcaacatctt
    22681 tttctgcgta atatcaaagt gatgcaggaa tcctctgttg gacccgcatt gattatgaac
    22741 ttcgacatgc gcaaagacgt tcgaggcgtc tttatggcga aagaagaaac actgctgtct
    22801 cttgcaaatg ttcatgcggt gaatgagaaa ggacaaagct ccgtcgatat cgacaggatt
    22861 aatcaccata ttgttaatgt ggaaaagatt aactttagat tgccggaacg gagagagtag
    22921 atttgcgacc attcataaac ttactggata taatgttcta aaagattata atcctcacac
    22981 taactgttaa agctggggct gggcagcatt gactactcag ggaaggtact catctttatc
    23041 ttgtgtttct tacgtaacaa ccagactaaa gtgatttatg aacgataacg ttttgctcat
    23101 aggagcttcc ggattcgtag gaacccgact acttgaaacg gcaattgctg actttaatat
    23161 caagaacctg gacaaacagc agagccactt ttatccagaa atcacacaga ttggtgatgt
    23221 tcgtgatcaa caggcactcg accaggcgtt agccggtttt gacactgttg tactactggc
    23281 agcggaacac cgcgatgacg tcagccctac ttctctctat tatgatgtca acgttcaggg
    23341 tacccgcaat gtgctggcgg ccatggaaaa aaatggcgtt aaaaatatca tctttaccag
    23401 ttccgttgct gtttatggtt tgaacaaaca caaccctgac gaaaaccatc cacacgaccc
    23461 tttcaaccac tacggcaaaa gcaagtggca ggcggaggaa gtgctgcgtg aatggtataa
    23521 caaagcacca acagaacgtt cattaactat catccgtcct accgttatct tcggtgaacg
    23581 caaccgcggt aacgtctata acttgctgaa acagatcgct ggcggcaagt ttatgatggt
    23641 gggcgcaggg actaactata agtccatggc ttatgttgga aacattgttg agtttatcaa
    23701 gtacaaactg aagaatgttg ccgcaggtta cgaggtttat aactacgttg ataagccaga
    23761 cctgaacatg aaccagttgg ttgctgaagt tgaacaaagc ctgaacaaaa agatcccttc
    23821 tatgcacttg ccttacccac taggaatgct gggtggatat tgctttgata tcctgagcaa
    23881 aattacgggc aaaaaatacg ctgtcagctc tgtgcgcgtg aaaaaattct gcgcaacaac
    23941 acagtttgac gcaacgaaag tgcattcttc aggttttgtg gcaccgtata cgctgtcgca
    24001 aggtctggat cgaactctgc agtatgaatt cgtccatgcc aaaaaagacg acataacgtt
    24061 tgtttctgag taatgcagac gagtcagaga aaaaaggacc aggttggact acgattattc
    24121 ggaaagaggt actgattaaa ttatcagtat gtggatttcc gagtaatccg agtaacccag
    24181 atgctgatgg tgtgtagtgt tatcgtaagg tttaatcaaa gttatgcgga actaagcttt
    24241 ggtatatagc cataacgttt ggctaaccta tggcaataat ttacgcacta tcagccactt
    24301 agaggtagga aaagaatgac gaatttaaaa gcagttatac cggtagcagg tcttgggatg
    24361 catatgttgc ctgccactaa ggcgattccc aaagagatgc taccgatcgt cgacaagcca
    24421 atgattcagt acattgttga cgagattgtg gctgcaggga tcaaagaaat cctcctggta
    24481 actcacgcgt ccaagaacgc agtcgaaaac cacttcgaca cctcttatga attagaatct
    24541 ctccttgaac agcgcgtgaa gcgtcaacta ctggcggaag tacaatctat ctgtccgccg
    24601 ggcgtgacca ttatgaacgt gcgtcagggt gaacctttag gtttaggcca ctccatttta
    24661 tgtgcgcgac ctgccattgg tgacaatcca tttgtcgtgg tgctgccaga cgttgtgatc
    24721 gacgacgcca gcgccgaccc gctacgctac aaccttgctg ccatgattgc gcgcttcaat
    24781 gaaacgggac gcagccaggt gctggcaaaa cgtatgccgg gcgacctctc tgaatactcc
    24841 gtcatccaga ccaaagaacc gctggatcgt gaaggcaaag tcagccgcat tgttgaattt
    24901 atcgaaaagc cggatcagcc gcagacgctg gactcagaca tcatggccgt tggtcgatat
    24961 gtgctttctg ccgatatttg gccggaactg gaacgtactc agcctggtgc atggggacgt
    25021 attcagctga ctgatgccat tgctgagctg gcaaaaaaac aatccgttga tgcaatgctg
    25081 atgactggcg acagttacga ctgcggtaaa aaaatgggct atatgcaggc gtttgtgaag
    25141 tatggactac gcaacttgaa agaaggggcg aagttccgca aaggtatcga gaagctgtta
    25201 agcgaataat gaaaatctga ccgaatgtaa cggttgataa ggaaataata tcggcagtga
    25261 aaaactatag atcaataaat atcactgctg ttgagctttt ataattaact atgctaccaa
    25321 tgaataa
//
LOCUS       DBFIFI010000051        24729 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.051, whole genome shotgun sequence.
ACCESSION   DBFIFI010000051 DBFIFI010000000
VERSION     DBFIFI010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24729
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.051"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..430
                     /locus_tag="N7B39_001993"
     CDS             <1..430
                     /locus_tag="N7B39_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001600411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HGU2421984.1"
                     /translation="SHERDVLEAVTLARSQANRASSDLQQTPGDEQKLQAWQQAQAQV
                     TRTLGQLTIISERYPELKSQELYQNLMVQLEGSENRIAVARGRYIKAIEQYNVTIRKF
                     PAVLTAKVMDYTPKKNYLPDDVAAVSKAPTIDFSQNANAH"
     gene            444..1118
                     /locus_tag="N7B39_001994"
     CDS             444..1118
                     /locus_tag="N7B39_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HGU2421985.1"
                     /translation="MRNFIFLMAFFCSSVFATQIPVPESPKYVNDLTGTLTNSEVNTL
                     TNQIKALTQKNYAQLVVLVVETTGDETIEQYATRVFDSWKPGDKDRDDGVLLLVAWQD
                     HTVRIEIGYGLEGIITDAQSGKIIRNSIIPAFKKGDLAGGLQKGISDIESRLTGNNLA
                     TITPTDHPLPFSGWWALLVWAIVLTFISARGYIKTLGVICFAAIVLAFVLPIAGFSGS
                     WGVLAT"
     gene            1352..2500
                     /locus_tag="N7B39_001995"
     CDS             1352..2500
                     /locus_tag="N7B39_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HGU2421986.1"
                     /translation="MRIFLLLLMLCGFQVFAERLPSVTDLTGTLTTEEQTALTQQLQT
                     LEQQNHFQVAVLVTPTTGGKNIKLAASQRYGVYHWKPVGEKRYGEGILILVVWPEGLA
                     SMKIGHGLEEMLPPEQAAQIVRYHMQPEFEKNNLFAGLTGGIESIAQFTHIKANLSPL
                     DALANHLFANPQRSLPCLAWTLLMIVAIVVLWLFASRPRRGIWMISMITPMVWFFSFQ
                     DDVIIRRVSVVCFSLLLATTCKQRLAVVFNMCRVFPMMLTPKNKNAKVKKQKKTPQVR
                     ESGRNSLFVVMLIIIVGWCLVFASNKDIAFISTIIGLIDHFIGPITIAGIVLLIIVAK
                     FTGNLKLGSGEKSNRKTGNRNAHSRSSSRNSFRGGGGSSGGGGSSVRW"
     gene            2497..3411
                     /locus_tag="N7B39_001996"
     CDS             2497..3411
                     /locus_tag="N7B39_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311665.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HGU2421987.1"
                     /translation="MIVMRLIFILFTLWCLPGLAQQIAVPELRQQVTDITGTLSTSEQ
                     QSLTQQLQDITHKTRAQVAVLVVPSTGDDSIEQYATRVFDSWKLGDKQRNDGILLLVA
                     WEDHAVRIEVGYGLEGVVTDLQAAKIIRDILIPAFKSDDLMGGLTLASENIGALLLNS
                     ELPEDRGDYYSINPPIPLSLAVIILLAVLSYFIVFTDPSNLPWITLTGAIYGMVFLYV
                     AEPGPWTNLIVACGMLTPFAIVPLVIFWLIVNKKLRAKYKKLSKDRASRKGSSSSSSG
                     GGSSGGGSGGGFSGGGGSSGGGGASGRW"
     gene            complement(3471..4769)
                     /gene="eno"
                     /locus_tag="N7B39_001997"
     CDS             complement(3471..4769)
                     /gene="eno"
                     /locus_tag="N7B39_001997"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HGU2421988.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(4857..6494)
                     /gene="pyrG"
                     /locus_tag="N7B39_001998"
     CDS             complement(4857..6494)
                     /gene="pyrG"
                     /locus_tag="N7B39_001998"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HGU2421989.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(6722..7513)
                     /gene="mazG"
                     /locus_tag="N7B39_001999"
     CDS             complement(6722..7513)
                     /gene="mazG"
                     /locus_tag="N7B39_001999"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HGU2421990.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(7584..7919)
                     /gene="mazF"
                     /locus_tag="N7B39_002000"
     CDS             complement(7584..7919)
                     /gene="mazF"
                     /locus_tag="N7B39_002000"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HGU2421991.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(7919..8167)
                     /gene="mazE"
                     /locus_tag="N7B39_002001"
     CDS             complement(7919..8167)
                     /gene="mazE"
                     /locus_tag="N7B39_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HGU2421992.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(8245..10479)
                     /gene="relA"
                     /locus_tag="N7B39_002002"
     CDS             complement(8245..10479)
                     /gene="relA"
                     /locus_tag="N7B39_002002"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HGU2421993.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(10527..11828)
                     /gene="rlmD"
                     /locus_tag="N7B39_002003"
     CDS             complement(10527..11828)
                     /gene="rlmD"
                     /locus_tag="N7B39_002003"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HGU2421994.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLEVEPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNCLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            11885..14641
                     /gene="barA"
                     /locus_tag="N7B39_002004"
     CDS             11885..14641
                     /gene="barA"
                     /locus_tag="N7B39_002004"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HGU2421995.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(14874..16214)
                     /gene="gudD"
                     /locus_tag="N7B39_002005"
     CDS             complement(14874..16214)
                     /gene="gudD"
                     /locus_tag="N7B39_002005"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HGU2421996.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(16235..17575)
                     /gene="gudX"
                     /locus_tag="N7B39_002006"
                     /pseudo
     CDS             complement(16235..17575)
                     /gene="gudX"
                     /locus_tag="N7B39_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417268.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
     gene            complement(17577..18929)
                     /gene="gudP"
                     /locus_tag="N7B39_002007"
     CDS             complement(17577..18929)
                     /gene="gudP"
                     /locus_tag="N7B39_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HGU2421997.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIAISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVVGQ
                     "
     gene            complement(19364..19813)
                     /gene="yqcA"
                     /locus_tag="N7B39_002008"
     CDS             complement(19364..19813)
                     /gene="yqcA"
                     /locus_tag="N7B39_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HGU2421998.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(19831..20613)
                     /gene="truC"
                     /locus_tag="N7B39_002009"
     CDS             complement(19831..20613)
                     /gene="truC"
                     /locus_tag="N7B39_002009"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HGU2421999.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGERSS"
     gene            complement(20613..20942)
                     /gene="yqcC"
                     /locus_tag="N7B39_002010"
     CDS             complement(20613..20942)
                     /gene="yqcC"
                     /locus_tag="N7B39_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HGU2422000.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(21564..22109)
                     /gene="syd"
                     /locus_tag="N7B39_002011"
     CDS             complement(21564..22109)
                     /gene="syd"
                     /locus_tag="N7B39_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HGU2422001.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLAPNLAEFLNQLKPLL"
     gene            22177..23025
                     /gene="queF"
                     /locus_tag="N7B39_002012"
     CDS             22177..23025
                     /gene="queF"
                     /locus_tag="N7B39_002012"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311681.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HGU2422002.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFQGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGEVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            23137..24501
                     /gene="ppnN"
                     /locus_tag="N7B39_002013"
     CDS             23137..24501
                     /gene="ppnN"
                     /locus_tag="N7B39_002013"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HGU2422003.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         6095 a         6489 c         5929 g         6216 t
ORIGIN      
        1 cagccatgaa cgagatgtac tggaagccgt aacattggct cgtagccagg ctaatcgcgc
       61 cagtagcgat cttcagcaaa ctccgggaga tgaacaaaaa ttacaagcct ggcaacaggc
      121 tcaagcgcag gtgactcgca ctctcggtca gttaacgata attagtgagc gttaccctga
      181 gttaaaatct caggaactct atcaaaattt aatggtacag cttgaaggaa gtgaaaatcg
      241 gattgccgtt gcgcgaggaa gatatattaa agccattgaa caatataatg tcactatccg
      301 caaattcccc gctgtattaa ccgcaaaggt catggattac acaccaaaga aaaattattt
      361 gccggatgat gtcgccgcag taagtaaagc cccaacaata gattttagcc agaatgctaa
      421 tgctcattaa gcggggaagt tctatgcgaa attttatctt ccttatggct ttcttctgtt
      481 catctgtgtt tgccacacaa attccagtac ctgaatcacc caagtatgtg aatgacttaa
      541 ccggcacatt aacaaacagc gaagtaaaca ccctgaccaa ccagattaaa gccctgacgc
      601 aaaaaaacta cgcacagttg gtcgtgttag tggttgaaac aacaggcgat gaaaccatcg
      661 agcagtacgc gacgcgggta ttcgatagct ggaaaccggg tgataaagac cgcgatgatg
      721 gcgttctact gttagtcgcc tggcaggatc ataccgtgcg tattgaaatc ggttacggac
      781 tggaaggtat tattacggat gcgcagtccg ggaaaattat tcgcaacagt attattcctg
      841 catttaaaaa aggcgacctg gctggtggtt tgcaaaaggg aatcagcgat attgaaagtc
      901 gtctgacggg gaataattta gcaacgataa caccgaccga tcaccctttg ccctttagcg
      961 gttggtgggc attgttagtc tgggcgatag tactgacgtt tatttctgcc agaggatata
     1021 tcaaaacgct gggagtaatt tgcttcgcag ctattgtact ggcctttgtt ttacctatag
     1081 caggcttcag cggaagctgg ggagtactcg caacatgact ctgcttcgcc acaccgttcc
     1141 tggctgtcgc catcgctttt acccccttcg gcaaaaaagt tcgggatttg atgcgtgatg
     1201 ccaaccagcc atcacgccat acaagaagca attcttcctc ccactattca tcatcgtcat
     1261 cctcttcatt cgacaatgac aattttagcg gtggtggcgg ttcatctggt ggcggtggcg
     1321 cttccgggcg ctggtaacaa aaggaattga tatgcgtatt tttctcttac ttctgatgct
     1381 atgcggtttt caggtattcg ctgaacgatt gccttcagtg actgacttaa ccgggacatt
     1441 aacgacagaa gaacaaactg ctttaactca acaattacag actctcgagc agcaaaacca
     1501 cttccaggtg gccgtgttgg ttacgccgac cacagggggc aaaaacatta aacttgcagc
     1561 ctcgcagaga tacggggtat atcactggaa acccgttgga gagaagcgat atggcgaagg
     1621 tatcttaatt ctcgtcgtat ggccggaagg gctggcgtca atgaagattg gacatggact
     1681 ggaagagatg ttgccgccgg aacaagctgc gcaaattgtt cgttaccata tgcagccaga
     1741 attcgaaaaa aacaatctct ttgctggatt aacgggcgga attgaaagca tcgcacagtt
     1801 tacccatatc aaagcgaacc ttagcccttt ggatgcttta gccaatcatc tgtttgctaa
     1861 tccgcaacgt tcgctgccat gtcttgcctg gacactgctg atgatcgttg ccatcgttgt
     1921 cctctggcta tttgccagcc gcccacggcg tggcatatgg atgatatcga tgattacccc
     1981 tatggtctgg tttttctcct ttcaggacga tgtcatcatt cggcgggtat cggtcgtttg
     2041 ttttagttta ttgttagcaa caacctgcaa gcagcgtctc gcggtagttt ttaatatgtg
     2101 ccgcgtattt cctatgatgc tgaccccaaa aaataaaaac gctaaagtga aaaaacaaaa
     2161 gaagactccg caggttcgtg aatcaggacg aaattcattg tttgtcgtga tgctgataat
     2221 tattgtggga tggtgcctgg tgttcgccag taataaagat attgcattca tctcaacaat
     2281 tattgggtta attgatcatt ttatcggccc aataaccatt gctggcatcg ttctgctgat
     2341 cattgtggcc aaatttactg gcaatcttaa gctggggtca ggagaaaaaa gcaacaggaa
     2401 aacggggaat cgtaatgctc attctcgctc ttcgtcgaga aattctttcc gcggtggtgg
     2461 cggttcttca ggaggcggcg gttcatccgt ccgttggtga tcgtaatgcg cttaatcttt
     2521 attctattca ctttgtggtg ccttcctggg cttgcccagc aaattgctgt gccggaactg
     2581 cgccaacagg tgaccgatat tactggtacg ttaagcactt ctgaacaaca atcactgaca
     2641 cagcaactgc aggatattac acataaaaca cgggcgcagg tcgctgtatt agtagtgccg
     2701 tccaccggag acgattccat tgaacaatat gctacaaggg tttttgatag ctggaaactg
     2761 ggagataagc agagaaacga tggtatttta cttctggttg cctgggagga tcatgctgtt
     2821 cgtattgagg ttggctacgg gctggaaggg gtggttaccg acctgcaagc ggcgaagatt
     2881 atcagggata tattgatccc tgccttcaaa agtgatgacc tgatgggtgg attaacactg
     2941 gcaagcgaaa atatcggcgc gcttctgttg aatagtgaat taccggaaga caggggggat
     3001 tattacagta tcaatccccc tattccatta tcgcttgctg taattatatt gctggcggta
     3061 ctttcatatt ttattgtttt taccgatccg tcgaatttac cctggatcac tctcactggc
     3121 gccatttacg ggatggtatt cctctatgtt gccgagccag gaccatggac caatttaatc
     3181 gttgcctgcg gtatgttaac gccttttgcg atcgtaccac tggttatttt ttggcttatc
     3241 gtcaataaaa agctacgcgc caaatacaaa aaactcagta aagatagagc ttcaagaaaa
     3301 ggttcttctt catcctcttc cggtggcggc tcaagtggcg gaggctcagg cggcggattt
     3361 agcggcgggg gcggttcttc tggcggtggc ggcgcgtccg gtcgctggta actcaaccat
     3421 aaaaaacgcc agcccgaagg ctggcatttt taaatcagat aaagtcagac ttatgcctgg
     3481 cctttgatct ctttacgacc gttgtacggt gctttttcgc ccagagcttc ttcgatacga
     3541 atcagctggt tgtatttagc aacgcggtca gaacggctca tagaaccagt tttgatctgg
     3601 cctgcagcag taccaacagc caggtcagcg atggtagcgt cttcagtttc gccagaacgg
     3661 tgagagataa ctgcagtgta gccagcgtct ttcgccatct tgattgcagc cagagtttcg
     3721 gtcagagaac cgatctggtt gaatttgatc aggatggagt tagcgatacc tttttcgata
     3781 ccttctttca ggatcttggt gttggttacg aacaggtcgt caccaaccag ctggattttg
     3841 tcgcccagaa ctttggtctg gtatgcgaaa ccatcccagt cagattcgtc cagaccgtct
     3901 tcgatggaaa cgatcgggta ctgtttggtc agttcttcca ggaagtgagt gaattcttca
     3961 gaggtgaatg ctttgttgcc ttcgccagcc agaacgtatt taccgtcttt gtagaattca
     4021 gaagctgcgc agtccatcgc cagagtgatg tctttgccca gttcataacc agcagcttta
     4081 acagcttcag cgataacagc cagagcttca gcgttggaac ccaggttcgg cgcatagcca
     4141 ccttcgtcac caacagcagt gttcatgcct ttcgctttca gaacttttgc caggtgatgg
     4201 aaaacttcag aacccatgcg gatggcttct ttcacagttt tcgcgccaac cggctgaatc
     4261 atgaattcct ggatatcaac gttgttgtca gcgtgctcac caccgttgat gatgttcatc
     4321 atcggaaccg gcatggagta tttgcccgga gtgccgttca gttcagcgat gtgctcgtac
     4381 agcggcatac ctttagcagc tgcagcagct ttggcgttag ccagagatac agccaggatt
     4441 gcgttcgcgc cgaatttgga tttgttttcg gtgccgtcca ggtcgatcat gatcttgtca
     4501 atgccagcct gatctttagc atctttgcca atcagcgcct gagcgatcgg gccgtttacc
     4561 gcagcaacag ctttggttac gcctttaccc aggaaacggg atttgtcgcc atcgcgcagt
     4621 tccagagctt cacgggaacc agtagaagca cctgacggag cagctgccat accgacgaaa
     4681 ccaccctcca gatgtacttc ggcttcaaca gtcgggttac cacgggagtc gatgatttca
     4741 cgaccgatga tttttacgat tttggacatt aggttttcct caagtcacta gttaaactga
     4801 aactccagac aaacaacgcg tacccagggt acgcgttgcc gttctaactt ttttacttac
     4861 ttcgcctgac gtttctggaa ctcgctggcg gctttcacaa agcctgcaaa cagcgggtga
     4921 ccatcacgtg gagtagaagt aaactccgga tggaactggc aagccacgaa ccacgggtga
     4981 ttcggaactt cgatgatctc gaccaactga tcatccccgg aacggcccgc aacgcgcaga
     5041 cctgcatctt caatctgttt caacagcatg ttgttgactt cgtaacggtg acgatgacgc
     5101 tcaacaattg tcggcgcatt gtacagctgg cgaaccaggc tatcgtcaac caactggcac
     5161 tgctgtgcgc cgagacgcat ggtaccgccg agatcgctct tctcgctacg aacttcaacg
     5221 ttgccgtttt catcgcgcca ctcggtaatc agcgccacaa ccgggtactt acagtctggc
     5281 acaaattccg tagagttggc gttctccatg ttggcaacat ggcgagcgta atcaattaac
     5341 gccacctgca tacccaggca aatgcccaga taaggaatat tgttctcacg cgcaaaacgc
     5401 gcggtcgtaa tcatgccttc tacgccacga tagccgaaac cgccaggtac gaggattgca
     5461 tccagacctt taaggatttc aacgccgcgc gtttcaacat cttgtgaatc gatcagtttg
     5521 atgttgacgc tgacacgatt cttcagccca ccgtgtttca gtgcttcaat cacggatttg
     5581 taagcgtccg gcagttcaat gtacttgccg accataccaa tggtgacttc acttaccgga
     5641 ttcgcttctt cgaagataac ctgttcccat tcggacagat ttgcttccgg gcagtttaag
     5701 ctgaatcgtt tacaaatata atcgtccagc ccctgagatt tcaacaggcc cggaatttta
     5761 tagatggaat cgacgtcttt cagagaaata accgcttttt ccggaacatt acagaacaat
     5821 gcaatcttcg cacgttcgtt cgccggaacg gcgcgatctg aacgacaaat caggatgtca
     5881 ggctggatac cgatggagag cagctctttt acagagtgct gagtcggttt ggttttgact
     5941 tcaccagacg ctgccatgta cggcaccagc gtcaggtgca taaacagagt gtgctcacgg
     6001 ccaatttcaa cagccatctg gcgaatcgct tcgaggaacg gcagggattc gatatcacct
     6061 actgtaccgc cgatttcgac cagtactacg tcatgacctt cgccaccttc cagcacgcgc
     6121 tctttgattg cgttagtgat gtgcggaata acctgtacgg ttgcgccgag gtagtcaccg
     6181 cggcgttctt tacgcagaac gtcagagtag atacgacccg tggtgaagtt gttgcggcgg
     6241 ctcattttgg tacggatgaa acgctcgtag tgccccaggt ccaggtcggt ttcagcgccg
     6301 tcttcagtaa cgaacacttc cccgtgttgg attgggctca tagtacctgg atcgacgttg
     6361 atgtacggat ccagtttcat gatggtcaca ttgaggccac gggcttcaag aatggctgcg
     6421 agggaggctg cggcaatgcc tttacccaga gaggatacga ccccgccggt cacaaaaata
     6481 tagttcgttg tcatgctgaa cctgagaagt taggttgaaa agacgatgga ataaccagga
     6541 cgggaaagca gtatacccga acatgacctg tgccacaaac tttcattatc cctcctcttc
     6601 gccagcgcac tattgaaatc aggagtgaga aaatagcccc tttggggtaa atgtttttga
     6661 cgcaaatcaa gcgcttgtca tttaaaaaat gacacaaatg gcgcttgacc gcgtaattcc
     6721 cttagagatc aatttcctgc cgttttacct gttgccagac ttcttccatt gtttcgaggt
     6781 caacacctgt catttccagt ccacgcgcgg caacaatacg ctccacttcg cgaaaacgac
     6841 gctcgaattt ttcgttcgct ttttgcaatg cgatttccgc ttttgtccct aaatgacgag
     6901 ccagattgac cgtggcaaac agcaggtccc ccatttcctc ctccagttta gcctggtcga
     6961 caacagcctg ccgcgcttcg tacatcacct cgtcgatctc ttcgtagact ttatcgacta
     7021 ccggaccaag cgtcgtccaa tcgaagccaa cgttggcgca acgtttctgg attttttgcg
     7081 cacgcattaa agccggtaaa ctacgaggaa tatcgtccag cgccgaatgc tgcgctttct
     7141 gcgcgcgctc ttcggttttg atttgctccc aacgggcaag cacttcacta ctgttttcgg
     7201 cagaactgtc agcaaaaaca tgcggatggc gacgctctaa tttatcgcta atcgcagcgc
     7261 aaatatcatt aaagtcaaag cgcccttctt cctgagccat ttgggcgtaa aacaccacct
     7321 ggaacagcag atcgccgagt tcaccgcgca gatcgtcaaa atcttcacgg gcgatggcgt
     7381 ccagcacttc gtaggtttct tcaagggtgt aaggcgcaat ggtggcaaat gtctgctctt
     7441 tatcccacgg gcagccgttt tccgggtcgc gcaggcgctg cataatagtg agcaaacggt
     7501 cgatttgatt cattagaatg tcctgaaaat tgcgggtccg tcaggtggaa acctgtgacc
     7561 agaatagaag tgagttagta acactaccca atcagtacgt taattttggc tttaatgagt
     7621 tgtaattcct ctggggcaac cgttcctttc ttcgttgctc ctcttgcccg ccaggcgata
     7681 ctttttacct gatcagctaa cgctacgcca tcacgttcct gaccggataa aacaacttcg
     7741 aacggatatc cttttgattg cgttgtacaa ggaacacaca gacacatacc tgttttgttg
     7801 ttgtacatga acggactcag gacaacagcc ggacgatgtc cggcttgctc gctacctttt
     7861 gtcgggtcaa aatcaaccca aatcagatcg cccatatcgg gtacgtatcg gcttaccatt
     7921 accagacttc cttatctttc ggctctcccc agtcgatatt ctcgtggagg ttttccggcg
     7981 tgatgtcgtt gaccagttca gcaagcgtaa atacgggctc tttacgcact ggctcaataa
     8041 ttaatttgcc atccaccagg tcaatcttca cttcatcatc aatattcaga ttgagcgcct
     8101 gcattaacgt agccgggatc cgcaccgccg gtgaatttcc ccaacgcttt acgctactgt
     8161 ggatcataac cctttcctca aaccactatc atatgtagat acagtatata tcaatctaca
     8221 ttgtagatac gagcaaattt cggcctaact cccgtgcaac cgacgcgcgt cgataacatc
     8281 cggcacctgg ttgagtttac ccagcacgcg ccccagcact tgcaggttgt aaatctcaat
     8341 ggtcatgtcg atggtcgcca gttgctgttt ggtgtcgcta cggctggcaa cgccaagcac
     8401 gttcaccttc tcgttggcga gaatggtcgt gatatcacgt aacaacccac tacgatcatt
     8461 agccaccacg cggaccacca gcgaatatcc ggcggagtag ctctcacccc ataccgcgtc
     8521 aacaatgcgt tctggcgcat gggagcgcag ttccgccagt tgttcgcaat cggcgcggtg
     8581 tactgaaata ccgcgtccct gggtaatgaa gccgacaatc tcatctccag gaatcggctg
     8641 gcagcagcgc gcgatgtggt gcatcaggtt accaacacct tcaaccacta cacgaccgtt
     8701 atctttactg cggttttgcg gcgtgtagct tttttgctga agctgtttca gcgcggcggc
     8761 gtcctgctct tcggcactcg gcttattaaa ttgcgattgc aggaagttca ccatctgatt
     8821 gagacggata tccccgccac caatcgccgc cagcaactcg tcgacatcat tgaagttgta
     8881 acgcggcagc agatgttttt ctgcttcttt caggctgatc cccagatgtt ccagctcgtc
     8941 gtcaaggatt tgccgcccag ccagaatgtt tttgtcacgg tcctgtttac ggaaccaggc
     9001 gtgaattttc gaacgcccac ggctggttgt gacgtaaccg aggtttgggt ttaaccagtc
     9061 acggctgggg ttcggctgtt tctgggtgat aatttcaatc tggtcgccca tttgcagctg
     9121 gtaggtgaac ggcacaatgc gcccgccaat ttttgccccg atacagcggt gtccgacatc
     9181 actgtggatg tggtaagcga agtccagcgg cgttgatccc gcaggcaaat caacgacatc
     9241 acctttaggc gtaaagacgt acacccggtc gtcaaagacc tggctgcgta cttcgtcgag
     9301 catttcgccg gaatcagcca tctcttcctg ccacgcaatc agtttacgca gccaggcaat
     9361 ccggtcttca tgtcccgaac gtgcgccgcc agcagccgcg ccctctttat atttccagtg
     9421 cgcagcaaca cccaactctg catcttcatg catctgtttg gtgcggattt ggatctcaac
     9481 ggtttttcca cccggcccca gaaccacggt atgaatagac tgataaccgt ttggtttcgg
     9541 gttagcgacg taatcgtcaa actcatccgg caggtggcga tagtgagtgt gcactatccc
     9601 cagtgcggca taacaatcct gtaaacgctc ggcgacaata cgtaccgcac gcacatcaaa
     9661 cagctcatcg aaggcgaggt tctttttctg cattttgcgc cagatgctgt agatgtgttt
     9721 cggacgacca tacacttcag ctttaacgcc ttcagctttc atctcagcgc gcagatgacc
     9781 gacaaactct tcgatatagt gttcgcggtc gagacggcgt tcatgcaaca gttttgcgat
     9841 gcgtttgtat tcggtcgggt ggagataacg gaagcagtaa tcttccagct cccatttcag
     9901 ttgcccaatc ccaagacggt ttgccaacgg cgcgtagata ttggtgcact cttttgccgc
     9961 cagtacgcgt tcatcttccg gcgcatcttt tacttcacgc agatgagcaa tacgctccgc
    10021 cagtttgatg accacgcagc gaaaatcatc gaccatcgcc aataacatcc ggcgaacgtt
    10081 atcgacctgt tcggaggaaa cagaatcagt gtgcgtcgct ttcagctggc ggatcgccgc
    10141 catatcacgc acgccgtgaa taaggttaac gaccgactta ccgacgctct cacgcagcac
    10201 atcttcgctg actacgttgg catcagccag agggaacagc agcgccgccc gcagcgtgtc
    10261 aatgtccata cttaatgtcg agaggatctc caccatctca acaccacgcc acaataacag
    10321 actggcatcc ggatgcccct gcgtctgttg cagacaatac gcccaggttt cggctaagca
    10381 ctcacacgac ttctggctgg taatacccag acttgcgatc catttttccg gatcaaattc
    10441 accagcctta ttgatatgtg cacttcttac cgcaaccatc gtcctctcct ttagggacca
    10501 gacctgccga aatcggcaaa tcgcaactat ttaacgcgcg agaaaagtac catcgattcc
    10561 agatgtcccg tgtgtgggaa catatccagc atcgccagtc gcgcaatggt atatcctgct
    10621 tttaataacg cttcgctatc ccgagccagc gttgcagggt tacaggatac ataaactata
    10681 cgaataggtt ccagttttat aatttgctgc ataacacctg cggcacctgc tcgcgccggg
    10741 tccagcaaca ctttatcgaa gccgtttttc gcccacggct gctttgtgac atcttcttca
    10801 agattttcgt gataaaacgt cacattctgt aagcagttaa gacgcgcatt ctgctggcct
    10861 ttttccacca gcgccggaac accttctaca cccaccacac tggcagcttg tgtcgccaat
    10921 ggtagtgtaa agttgcccat accgcagaac agatccagta cgcgatcttc aggttccacc
    10981 tccagccatt ccaacgcacg cgctaccatt ttttggttca cacccgcatt aacctgaata
    11041 aaatcgcgcg ggctaaaagt taagcgcaac ccgtttgagt cataccaggg catctcacca
    11101 gagacggttt cgagtatctc actatcgggg gcgagataca gatccaggcc ttcagaatgc
    11161 gaaaagcgtt ccagtttttc gcgatctgcc gaacttagcg gtgcggtatg gcgcaaaatc
    11221 atcagcgtgc cgctggttgc ctgtaccagt tcaacatgac caagatggcg catagcttgt
    11281 aagctgccca ggcatgccct gactttgggc agcaatgctt caagttgggg cactaaaatg
    11341 ggacattgtt taacgtcgac aatgtcactg gagcccgctt tgcgaaaccc catctgaagt
    11401 tgctgtgttt tcggtaagta gttcagactt aaacgcgcgc gacggcgata gccccaggga
    11461 acatcggcga tcacttcaga gacttcgtgt ttcattaatc gggcgagtgc cgcacttttg
    11521 cttcgctgct gtaaatccac gctggcgtgt tgttgctgac agccgccgca tacgccaaaa
    11581 tgaggacagc gtggcgtttc gcgttccggg ctatcgctta accggcgtac gactttagcg
    11641 cgggcatact gttttttatc ctcagtaaca gtaacttccg cgttttcctg cggcaataat
    11701 ccggggataa atagcgtttt gccgttatgt cgcgccacgc cctgaccaaa agagtcgagg
    11761 tcgttgactg aaacggttat gatctgacgc gtcgtcgtgc gtcgttttgc agagtagaat
    11821 tgcgccattg gcgagacttt ctcaatttaa cagtgtgacc ttaattgtcc cataacggaa
    11881 ctccatgacc aactacagcc tgcgcgcacg catgatgatt ctgatcctgg caccgaccgt
    11941 ccttattggt ttattgctga gtatcttttt cgtcgtgcat cgctataacg acttgcagcg
    12001 tcaactggaa gatgccggtg ccagcattat tgagccgctt gcagtttcta ctgaatatgg
    12061 catgagcctg caaaatcgcg aatctatcgg tcagttaata agcgtactgc atcgtcgcca
    12121 ttccgatatt gttcgcgcga tttcggttta tgatgaaaat aaccgactct ttgtcacctc
    12181 caattttcat cttgatccct catcaatgca gctcggcagc aacgtgccgt ttcctcgcca
    12241 gctcactgtc actcgtgacg gcgatattat gatcctccgc acgccgatta tttctgagag
    12301 ttactccccc gacgaatcgc ccagtagcga tgccaaaaat agtcaaaata tgttgggata
    12361 tattgcgctt gagctggatc ttaaatcggt tcgcttgcag caatataaag agatctttat
    12421 ttccagcgtg atgatgctgt tttgtatcgg tattgcgctt atttttggct ggcgcttaat
    12481 gcgcgatgta accggtccga ttcgcaacat ggtgaatact gtcgaccgca tccgtcgcgg
    12541 gcaactcgac agccgagtgg aaggatttat gctcggcgag ctggatatgc tgaaaaacgg
    12601 tatcaactcg atggcaatgt cgctggctgc ttatcacgaa gagatgcagc acaatatcga
    12661 ccaggcgacg tccgatctgc gtgaaacgct ggagcagatg gaaattcaga acgttgagtt
    12721 ggatctggcg aaaaagcgcg cccaggaagc ggcgcgtatt aaatccgagt ttctggcaaa
    12781 tatgtcacac gagctgcgta caccactgaa tggtgttatt ggttttaccc gcctgacgct
    12841 gaaaacagaa ttaacaccaa cgcagcgcga tcacctgaat acgattgaac gttcggcaaa
    12901 taatttgctg gcaattatta atgatgttct cgacttctcg aaactggaag caggtaagct
    12961 gattctggaa agtattccat tcccactacg cagcacgctg gatgaagtcg ttactctgct
    13021 ggcacattct tctcacgata aagggttgga actgacgctc aatattaaaa gcgacgtgcc
    13081 tgataacgtg atcggcgacc cactgcgatt acagcaaatc atcactaacc tggtggggaa
    13141 tgcaattaaa ttcaccgaga atggcaacat tgatattctg gtagaaaaac gtgcgctgag
    13201 taataccaaa gtgcagattg aagtgcagat tcgggatacc ggcattggta ttcctgaacg
    13261 cgatcaatcg cgcttattcc aggccttccg acaggctgat gccagtattt cccgccgtca
    13321 tggtggcacc gggctggggc tggtgattac acaaaaactg gttaatgaaa tgggcggcga
    13381 tatttcgttc catagccagc cgaatcgcgg ttcaactttc tggttccaca ttaatctcga
    13441 tctgaacccg aacattatta ttgaagggcc atccacccag tgcctcgccg gtaaacgcct
    13501 ggcctatgtc gaaccaaact ccgcagcagc gcaatgcacg ctggatattt taagtgaaac
    13561 gccgctggaa gtggtttata gcccaacgtt ctccgcgctg cctcccgcgc attacgacat
    13621 gatgttgtta ggcatcgcgg tgaccttccg cgagccgcta acaatgcaac atgagcgatt
    13681 agcgaaagcg gtatcgatga ccgatttcct gatgctggca cttccttgcc atgcacaagt
    13741 caatgctgaa aaactcaagc aagatggtat cggcgcgtgt ctgctgaaac cattaacacc
    13801 tacgcgcctg ttgcctgccc tgacggaatt ttgtcatcac aaacaaaaca cgcttttgcc
    13861 tgtaaccgat gaaagtaagc tggcaatgac agtcatggcg gttgatgaca accccgctaa
    13921 cctgaaactt atcggcgcat tgctggaaga tatggtgcaa catgtggaac tttgcgatag
    13981 cgggcatcag gcggttgaac gggcgaaaca gatgccgttc gatttgatct taatggatat
    14041 tcaaatgcct gacatggatg gcattcgggc ctgcgagctc atccaccagc tcccccatca
    14101 gcaacaaacg ccggttatcg cggtaacggc gcatgcaatg gccgggcaaa aagagaagct
    14161 gcttggcgca gggatgagcg attatctggc gaaaccgatt gaagaagagc gattgcataa
    14221 tttgttgttg cgctacaagc ctggcagcgg tatttcgtct cgcgtcgtga cgcccgaagt
    14281 caacgaaatt gtggtgaacc cgaatgcgac cctcgactgg caactggcac tacgccaggc
    14341 agcaggaaaa accgatttag cgcgcgatat gctgcaaatg ttactcgatt tcctgcctga
    14401 agttcgcaac aaagttgagg aacagctggt tggagaaaac ccggaaggcc tggttgattt
    14461 gattcataaa ctgcatggca gttgcggcta tagcggtgtg ccgcgtatga agaatctctg
    14521 ccaacttatc gaacaacagc tacgtagcgg tactaaagaa gaagatttgg aaccggagct
    14581 gctggaactg ttggacgaga tggataatgt cgcgcgcgaa gccagcaaaa ttctcgggta
    14641 acggacgtct tgtcgagtag caatcggctt tttatgacgc tgttttcaga ttggtgacaa
    14701 agtgcctgtg tttatgccgg atgcggcgtt aacgccttat ccggcctaca aaaccaatta
    14761 aattcaataa attaaaaagc atgtaggcct gataagcgta gcgcatcagg caattttgtg
    14821 tttggcatca gtctcaaacc ggctccagat agagccggtt ttggtttttt gtcttaacgc
    14881 accatgcacg ggcgcttgtt atcgaacgtc cagccaggaa tcagatactg cattcccatc
    14941 gcatcgtcac gcgcgccaag cccgtgtttc tgatacagct catgggcttt catcacttga
    15001 tccatatcga tttctacacc cagccccggt ttttctggca cctgtaccag cccgcctttg
    15061 atctcaaacg gttctttggt caggcgctga ttaccttcct gccagatcca gtgcgtatca
    15121 atagcagtaa ttttacccgg tgcagcggcg gcaacatggg taaacatcgc cagggaaata
    15181 tcgaagtggt tgttagagtg tgaaccccag gtcaggccaa attcatggca catttgcgcc
    15241 acacgtaccg aaccttgcat tgtccagaaa tgcggatccg ccagcgggat atcaacggat
    15301 tgtaacgaga gcgtatggcc catctgccgc cagtcggtgg cgatcatatt ggttgcagtc
    15361 ggcaggccag tcgcgcgacg gaactctgcc atcacttcac gcccggagaa accttgctcc
    15421 gcaccacacg gatcttctgc ataagccagc gaacctttca ggtatttgcc gattttaatc
    15481 gcttcgttca gcgaccaggc accgttagga tcgagcgtaa tacgcgcctg cgggaagcgt
    15541 tgcgccagtg ccacaataga ctcggcctct tcttccccgg ccagtacacc gcctttcagt
    15601 ttgaaatcgt tgaagccata tttttcatac gccgcttccg ccaggcgcac caccgcatcc
    15661 ggcgtcatcg cttcttcatg acgcaggcga taccagtcgc atgagtcatc cggctggctt
    15721 tgatacggca gaggtgtggc tttgcgatta ccgacgaaga acagataacc gagcatttcg
    15781 acttcgctac gctgttgacc atcgcccagc agggatgcca cgttaacccc cagatgctgc
    15841 cccagcagat ccagcattgc cgcttctatc ccggtaacta catgaatagt ggtacgcaaa
    15901 tcaaatgtct gcaaaccgcg cccgccggca tcacgatcgg caaaagtatt acgcaccagc
    15961 gtcagaacgt ttttgtattc acccagcgtt ttacccacta ccagcggaat tgcatcttcc
    16021 agcgttttac ggattttctc gccgccggga atttccccta cgccagtgtg accagaatta
    16081 tctttgataa tcacaatatt acgcgtgaag aacggtgcgt gtgcaccact cagattcatc
    16141 agcatactgt catggcccgc caccgggatg acctgcattt cagtaacaac aggcgtcgta
    16201 aattgagaac tcatatttat gtcctttttc agaatcaatg acggccgaaa acgggacgtt
    16261 tacggtcaaa ggtccagccg gggatcaggt actgcatcgg gcctgcgtcg ttacgcgcac
    16321 cgccaggcag acgtttatag gcctcatgtg ccttttgtac ctgttcccag tccagttcca
    16381 cgcccagacc gggcgcatca ggaacggcaa tttttccgtt tttaatctcc agcggatttt
    16441 gggtcaggcg acaatcgccc tcctgccaaa tccagtgggt atcgatagcg gtaggattac
    16501 ccggtgccgc cgcgcccaca tgggtaaaca tcgccagaga gatatcgaaa tggttgttgg
    16561 aatggcagcc ccaggtcagc ccccagtcgt cgcaaagctg cgccactcgg actgcaccgg
    16621 aaagcgtcca gaagtgcgga tcggcaagtg gaatatctac cgcattgagc atcaccgcat
    16681 gacccatttc gcgccagttg gtggcgatca tattagtcgc gacgggcaag ccggtcgccc
    16741 gccgaaactc cgccatcact tcacgtccgg agaagccctg ttctgcgccg catggatctt
    16801 cggcataggt aagaacatca ttcagccctt tgcacagaga aatggcttca tcaagcagcc
    16861 atgcaccgtt gggatcaacg gtaatccgcg catccgggaa gcgtttcttc aatgcacgaa
    16921 cagtgtcgat ttcttgctcg ccaggtaaca cgccgccctt aagtttgaaa tctttaaagc
    16981 cgtagcgatc ctgtgaggct tccgccagac gcacaacggc ttcgctgttc atcgctttct
    17041 gatggcgcaa ctgataccac tcatggttgc ccggcgtatt ttccagataa ggaagatcgg
    17101 ttttggtccg atcaccgata taaaacagat aaccgaggac ggtaatagca tcgcgttgct
    17161 tgcctggccc taacagttcg cagaccggaa cattcagcgc cttacctagc aggtcaagca
    17221 aggcggcttc cagcgccgcc acggcgttaa cgcgcaattc aaaggtccag gcccctttac
    17281 cgaaggtatc aaaatcggct gcttgattac ctttatgcac ctgctgaacc actttattca
    17341 ggcgcgcaac ttcctagccc agcaccatcg gaatagcatc gaccaatgtc tgataaatca
    17401 cctctccgcc tggcgcttca ccaatgccgg tatgcccggc gttatcggtg agtaccacaa
    17461 tattgcgagt gaaatatgcg ttatgcgccc caccaatatt aagcaacatg ctgtcgtacc
    17521 cggcgaccgg aatgactttc atatcagtaa taacaggact ggattgtgtc gtcatcttat
    17581 tgccccacga caggtttcag ctcgatacgc ttgatatcgc ccaccagcac caggtagctc
    17641 agtaccgcga ttaaggcatg aacaccaaca taaatcagcg ccccattaaa cgagccagtc
    17701 gtgccaacga tataaccaat tgcgattggc gtgacgatgc cagaaatgtt accgaacatg
    17761 ttgaacaggc caccggaaag accgctgatc tcttttggcg cggtatctgc cattactgcc
    17821 caacccagcg ccccaatgcc cttaccgaag aaggccagcg ccataaagcc gatgatcatc
    17881 cactcaacgt tgacgtagtt gcagaacacc atcaccatcg acagcaacat gcccattacg
    17941 atcggtgttt tacgcgcaat gttcagcgat cccgtgcggc gcatcagcca gtcggaaata
    18001 atcccaccca gcacaccacc gataaaacca caaaccgccg gaacggaagc cacaaagccc
    18061 gctttcagaa tcgacatccc acgcgcctgc accagataaa ccggaaacca ggtaatgaaa
    18121 aagtaagtca gggcgttgat acagtactga ccgatataaa cgccgatcat catccgtgac
    18181 ccgagcagtt gtttgatctg cccccacttc acgctgaacg gcactttaac tttggtgttt
    18241 tgctgatcca tattgatcag cgcgccaccc gcggcgatgt actccagctc tttctgattt
    18301 acccccggat gttgatttgg ctcgtggatg actttcaacc agataaagct gatggcaatg
    18361 cccagaccgc ccataaagaa gaagacgtgt gaccagccca cttcatgcgt cagccagccc
    18421 ataatcggcg cgaagatcac cgttgcgaag tattgagcgg agttaaaaat cgacaccgcc
    18481 gttccccttt cctgcgccgg aaaccaggcc gcaacaatgc gactgttgcc ggggaaagat
    18541 ggcgcttcag caagcccgac caggaagcgc agagtaaaca gggcaacgat aatgccgaat
    18601 ccactaaaga tatcgacgaa gccttgcagc aaggtaaaca tcgaccagat aaagatcgac
    18661 cagaagtaga cgcgttttga accaaaacga tccagcaacc aaccaccagg gatctgcccg
    18721 ataacataag cccatgagaa ggcagagaac acatagccca ttcccacggg atcaaggccg
    18781 atatctttgg ccatttccga accggcaata gaaagcgtag cgcggtcgcc gtagttgaag
    18841 gatgtgacga taaacaacat caccactatc cagtaacgag cattcgtgcg tttttccaca
    18901 ctgctcgcag cctgacttaa agaactcatt gttgcactcc tgaaaattcg cgttagccac
    18961 gctcactctg gactgcgaca tcgccaggaa atcagaggtg acgtagggtg ttttttgccg
    19021 tttttatagg tcgttcgccg aatacggcgc gtgtttatat ctggcaatag cagtataaaa
    19081 agcgcgccat agcggctcac cgtgcaacaa cacaacatta atgcgttcaa tgaggcccga
    19141 ttttggcatt agccctggac ggtggaatcc acttcacgga aatgaaaaca agaacaagaa
    19201 aggaagggtt aaaacgaaga aataaagaag agtatgaaat ggatcgcttg actccaggca
    19261 aacgccagta aaaatccgcg ctatgaagca gtttttactg gcatttgcct gaaaagattc
    19321 gattcagcac cgctaaaacg acatttaccg ctcgctgaac atatcaggac aacagcgtgc
    19381 cccactgttc gacccacgga tttgattccg tttccggttc cgggttctca ctggcgtcaa
    19441 tcaacagcat ttcgccaacc cgctgagcgc tctgttcctg cagcaaggca tcgaactgtt
    19501 tgccgccatt gcagaaattc acataactac tgtcgccgag cgcaatcacg ccataacgca
    19561 gattcggctg gaagcccaga ctatctttga ttccctgaaa gagcggcaca atgctatcag
    19621 gaaggtcgcc ctgcccggtc gtggacgtaa ccaccagaac atacttatcc tgatagggca
    19681 gccagtcgct taattcagga tcttcaaata ccgttgcttt gtggccctgt gcggtcagaa
    19741 ttgcttctgc ttcttccgcc actaacagcg aatttccgta catggtgccg acaaaaatac
    19801 caatttccgc cataccgttt tctccctgga ttaggaactt ctctctccat cctgacccga
    19861 tggcgcactg aactcaaccc tttcattttc aggaagcaga ccgcgccagc caaattgtga
    19921 taacgcctgc atccaggtgt cgtccaggcc cgcgtgaata gtcagcggct caccagtaaa
    19981 aggatgcgtc aatgacaact gactggcatg cagcattaac cgctgaagac caaaatgctc
    20041 agcaccgctg cgattctggc gtaaatcgcc atgtttgcta tcgccaataa tcggatgacg
    20101 caaatgggca agatgtcggc gaagctgatg tttgcgtccg gttttcggct ccagttccac
    20161 caggccgtag cgcgtggtcg ggtaacgtcc ggtcgctacc ggcatttcta cggtcgccag
    20221 accgcgataa tgcgtcactg ctggctgcgg gcctttatct tcgcgggcaa atttatcagc
    20281 gattttgtcc agttcttcca ccagtggata atccagcacc gcttcttcca tcaaccagcc
    20341 gcgcacaatc gcatggtaac gtttctggat ttggtgctgt tcaaactgtt gtgccagcag
    20401 ccgtccggcc tcgctggata atcccatcaa caacacacca gaagtgggtc ggtccagacg
    20461 atgagcagta aaaacatgct ggcctatctg gtcacgcacg gtttgcatga ccactacttt
    20521 ctcgtcgcga tccagccagc tacggtgaac cagccagccg gagggtttat ttaccgcaac
    20581 cagccattca tcctgataga gtatttccag cattagctcg catcatccgc aaaaagagca
    20641 tccagttttt ccaactcagc cagaataagc gcgcgttgcg gatggtccgt cgccagcgcc
    20701 atttcataat agggtgcaac ggcaaaagcg cccggtaacg gctggttgtt atttaacaaa
    20761 tcgtgcattc gcgggatcag cacccactgc aaccactcca gtggttccat ggtgtccata
    20821 aagaacggtt gggtactatt aaattgatgc ggctggggtt catcgtttcg ccagtgctga
    20881 tgttcacgca gtaacgcttc aagcgcctgc aactggagac gaacgcggtc atgagtggtc
    20941 ataaagcaac ctcaataaga aaaactgccg cgaaggatag caggaataaa aaaagggagc
    21001 actgtattca cagtgctccc ggttcgtttc gcagcattcc agctactttt gttgctccct
    21061 gctcatcctt gacaactttt cctctggcca tgcggccaat cgttcatcct gaactattgc
    21121 ttcctgctca caccaccccg atgtgatact tcatcctgaa gtgtcccagg ccttcctgac
    21181 ccaccgaatc atcctgaccg gttctcattc tccatcctgg aggtgtcctt taacgcgtcc
    21241 tgcgtcatcc tcttcgcttc atccagaagc ctttccctga aacaccatcc tggtgtgtcc
    21301 ttcagaagtg tcatcatcct gatgttcact tcgttgtctg actccctgtc gacgaagata
    21361 gaatcgtctt tttcgcgaac tcttacaagg cgcttacaga caatgcctta aggaaaattt
    21421 catatgaaag ttaggaatta acaagcaaca tgttgttttt aaatgaaaat aacgattctt
    21481 atctttttaa gtattcgctt atttcctggc gatctcctac agagtctgta agagatctct
    21541 cacaaccaga tggggtattt ggattacaga agaggcttaa gttgattaag gaattccgca
    21601 agatttgggg ctaaatgcgt ccgtttacgt gttcccagcg tctctttaca cacttcaccg
    21661 gagagattac acaccgagat gacctctaac tcttcttcca gcgtcgcgat aaatagcgtg
    21721 ggtggcagtt ttagcctttt ctgagtcacc agatgaccaa tcagattttc ctgcacccga
    21781 cggaagtcgt cttcactcca ggtttgcagc aatgtcagtt tgatatcgcc aaactgcgcg
    21841 tgcatatccc cggcaaactg agtcgtataa aaggtatgaa ttgtgggttg tatcacaata
    21901 tcaaaagcgc gttcaaccgc gtttacattt tgttccccgg taaacggctg gggttgccag
    21961 tatacggcat cttcagtagt tgaaataatg cacggtgaag gaacgccgta cagttcctca
    22021 cttaacggcc agcttttatg ctcttcatgc cacgcatcgc agtagcgtgc agtaaaatct
    22081 ttcagggctt gtgcggtcaa atcgtccact aatttctctc ttcacttaaa ccagatacac
    22141 ttgtcgttta gtttatctgg tttatgacgg tgaaacatgt cttcttatgc aaaccatcag
    22201 gcacttgcgg gcctgactct tggaaaatca accgattacc gggataccta tgacgccagc
    22261 ctactgcaag gcgttccacg cagcctgaat cgcgacccgc tgggtctgaa agcggataac
    22321 ctgccttttc aaggtacgga tatctggacg ctgtatgaac tttcctggct gaatgcgaaa
    22381 ggtttgccgc aggtcgctgt cggtcatgtt gaacttgatt acaccagcgt aaatctgatt
    22441 gagtcgaaga gttttaagct ctatctcaac agttttaacc agacgcgttt taataactgg
    22501 gatgaggtgc gccagacgct ggagcgcgac ttaagcactt gcgctcaggg tgaggttagc
    22561 gtggcgttat atcgtcttga tgaactggaa ggccagccga taggtcattt caatggcact
    22621 tgcattgatg accaggatat cactatcgat aactatgaat tcactactga ctatctggag
    22681 aatgccacca gtggtgaaaa agtagtggaa gagacgctcg tcagccacct gctgaaatca
    22741 aactgcctga tcacccatca accagattgg ggttcgatcc aaattcagta tcgtggacgc
    22801 caaattgaca gagaaaaact gctgcgttac ctggtctcat tccgtcatca caacgagttc
    22861 cacgaacagt gcgtggaacg catctttaat gacctgttac gcttctgcca gccagagaaa
    22921 ttgagcgttt acgcacgtta tacccgtcgt ggcggtctgg acattaaccc gtggcgcagt
    22981 aatagcgatt ttgtcccatc gaccacaaga ctggttcggc aataaatttt ttctcaattt
    23041 tgcgtgctgg attcacgcag aaggttgtga aaggtcatca ggcagggcta ttgtaatcaa
    23101 agggaatgac gatattcgtc ccataaggag tttttcttga ttacacatat tagcccgctt
    23161 ggctccatgg atatgttgtc gcagctggaa gtggatatgc ttaaacgcac cgccagcagc
    23221 gacctctatc aactgtttcg caactgttca cttgccgtac tgaactccgg tagtttgacc
    23281 gataacagca aagaattgct gtctcgtttt gaaaatttcg atattaacgt cttgcgccgt
    23341 gaacgcggcg taaagctgga actgattaat cccccggaag aggcttttgt cgatgggcga
    23401 attattcgcg ctttgcaggc caacttgttc gcggttctgc gagacattct cttcgtttac
    23461 gggcaaatcc ataataccgt tcgttttccc aacctgaatc tcgacaactc cgtccacatc
    23521 actaacctgg tcttttccat cttgcgtaac gctcgcgcgc tgcatgtggg tgaagcgcca
    23581 aatatggtgg tctgctgggg cggtcactca attaacgaaa atgagtattt gtatgcccgt
    23641 cgcgtcggaa accagctggg cctgcgtgag ctgaatatct gcaccggctg tggtccggga
    23701 gcgatggaag cgccgatgaa aggtgctgcg gtcggacacg cgcagcagcg ttacaaagac
    23761 agtcgtttta ttggtatgac agagccgtcg attatcgccg ctgaaccgcc taacccgctg
    23821 gtcaacgaat tgatcatcat gccggatatc gaaaaacgtc tggaagcgtt tgtccgtatc
    23881 gctcacggca tcattatctt ccctggcggt gtgggtacgg cagaagagtt gctgtatttg
    23941 ctgggaattt taatgaaccc ggccaacaaa gatcaggttt taccattgat cctcaccggc
    24001 ccgaaagaga gcgccgacta cttccgcgta ctggacgagt ttgtcgtaca tacgctgggc
    24061 gaaaacgcgc gccgccatta ccgcataatc attgatgacg ccgctgaagt cgcccgtcag
    24121 atgaaaaaat cgatgccgct ggtgaaagaa aatcgccgtg atacaggcga tgcctacagc
    24181 tttaactggt caatgcgcat tgcgccagat ttgcaaatgc catttgagcc gtctcacgag
    24241 aatatggcta atctgaagct ttacccggat caacctgttg aagtgctggc tgccgatctg
    24301 cgccgtgcgt tctccggtat tgtggcgggt aacgtaaaag aagtcggtat tcgcgccatt
    24361 gaagagtttg gtccttacaa aatcaacggc gataaagaga ttatgcgtcg tatggacgac
    24421 ctgctacagg gttttgttgc ccagcatcgt atgaagttgc caggctcagc ctacatccct
    24481 tgctacgaaa tctgcacgta atctcccctc ccccggtgac gtttgttgcc gggttttctc
    24541 gtttttggtc acttactcat caactcattt catttgttat atgaatgttt cttaccaccc
    24601 tcacgcgaca aatatcatca cagttaatat gtcatacaat ttatattgca acgcaaacgt
    24661 ttccctattt tcataaaccg tattttatcg ctatgaaaaa gaaattatcg ccatgattaa
    24721 ctaaaagta
//
LOCUS       DBFIFI010000052        24580 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.052, whole genome shotgun sequence.
ACCESSION   DBFIFI010000052 DBFIFI010000000
VERSION     DBFIFI010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24580
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.052"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(33..220)
                     /locus_tag="N7B39_002014"
                     /pseudo
     CDS             complement(33..220)
                     /locus_tag="N7B39_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase DNA-binding-containing protein"
     gene            complement(433..1092)
                     /gene="rluA"
                     /locus_tag="N7B39_002015"
     CDS             complement(433..1092)
                     /gene="rluA"
                     /locus_tag="N7B39_002015"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HGU2422004.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFRERVPKKQYVTRVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(1104..4010)
                     /gene="rapA"
                     /locus_tag="N7B39_002016"
     CDS             complement(1104..4010)
                     /gene="rapA"
                     /locus_tag="N7B39_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HGU2422005.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            complement(4174..6525)
                     /gene="polB"
                     /locus_tag="N7B39_002017"
     CDS             complement(4174..6525)
                     /gene="polB"
                     /locus_tag="N7B39_002017"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HGU2422006.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILRGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGAIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASALDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRIPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLVSSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEATKIG
                     RALVQHVNVWWAETLQKQQLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYFRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            complement(6600..7295)
                     /gene="araD"
                     /locus_tag="N7B39_002018"
     CDS             complement(6600..7295)
                     /gene="araD"
                     /locus_tag="N7B39_002018"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HGU2422007.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSIMTADDMVVVSIETGEVVEGAKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            complement(7495..8997)
                     /gene="araA"
                     /locus_tag="N7B39_002019"
     CDS             complement(7495..8997)
                     /gene="araA"
                     /locus_tag="N7B39_002019"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HGU2422008.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVKALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAAEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            complement(9008..10708)
                     /gene="araB"
                     /locus_tag="N7B39_002020"
     CDS             complement(9008..10708)
                     /gene="araB"
                     /locus_tag="N7B39_002020"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HGU2422009.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCTTGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKEQIDASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            11047..11925
                     /gene="araC"
                     /locus_tag="N7B39_002021"
     CDS             11047..11925
                     /gene="araC"
                     /locus_tag="N7B39_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HGU2422010.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            12011..12775
                     /gene="yabI"
                     /locus_tag="N7B39_002022"
     CDS             12011..12775
                     /gene="yabI"
                     /locus_tag="N7B39_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HGU2422011.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLVRHPLMPVYIDILRKVVGV"
     gene            complement(12862..13560)
                     /gene="thiQ"
                     /locus_tag="N7B39_002023"
     CDS             complement(12862..13560)
                     /gene="thiQ"
                     /locus_tag="N7B39_002023"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HGU2422012.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGALTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAVQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGMTNELL
                     SGKASASALLGITG"
     gene            complement(13544..15154)
                     /gene="thiP"
                     /locus_tag="N7B39_002024"
     CDS             complement(13544..15154)
                     /gene="thiP"
                     /locus_tag="N7B39_002024"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HGU2422013.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGDWS
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVTALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTMLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFSVIEKIPGRNVKTD"
     gene            complement(15130..16113)
                     /gene="thiB"
                     /locus_tag="N7B39_002025"
     CDS             complement(15130..16113)
                     /gene="thiB"
                     /locus_tag="N7B39_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HGU2422014.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPVVKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDIFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEKLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     regulatory      complement(16144..16243)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            16485..17054
                     /gene="espY2"
                     /locus_tag="N7B39_002026"
     CDS             16485..17054
                     /gene="espY2"
                     /locus_tag="N7B39_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector EspY2"
                     /protein_id="HGU2422015.1"
                     /translation="MKVRNPEQISIPASNTTKDPGLTNSQIIRMANLVKKTENMNIFE
                     ELWETLRNLFQSDKHSQTAARQILKDAFYFQNSDDYSKYFTGAVDGKARDKLTHWLIK
                     FNELKEYAKDPENMAAKASLSPEGTLCVSFFIGDEAIFTLELQLKKSTRTGGIDLSNA
                     YFNGVVICGIDCLEVDLSNAETNNSRWYD"
     gene            complement(17283..18941)
                     /gene="sgrR"
                     /locus_tag="N7B39_002027"
     CDS             complement(17283..18941)
                     /gene="sgrR"
                     /locus_tag="N7B39_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HGU2422016.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     ITSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQVDAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDPE"
     gene            19030..19161
                     /gene="sgrT"
                     /locus_tag="N7B39_002028"
     CDS             19030..19161
                     /gene="sgrT"
                     /locus_tag="N7B39_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HGU2422017.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELMQWEGSHSDY"
     gene            complement(19269..19874)
                     /gene="leuD"
                     /locus_tag="N7B39_002029"
     CDS             complement(19269..19874)
                     /gene="leuD"
                     /locus_tag="N7B39_002029"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HGU2422018.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFSQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMR"
     gene            complement(19885..21285)
                     /gene="leuC"
                     /locus_tag="N7B39_002030"
     CDS             complement(19885..21285)
                     /gene="leuC"
                     /locus_tag="N7B39_002030"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HGU2422019.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGILL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            complement(21288..22379)
                     /gene="leuB"
                     /locus_tag="N7B39_002031"
     CDS             complement(21288..22379)
                     /gene="leuB"
                     /locus_tag="N7B39_002031"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HGU2422020.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     IELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            complement(22379..23950)
                     /gene="leuA"
                     /locus_tag="N7B39_002032"
     CDS             complement(22379..23950)
                     /gene="leuA"
                     /locus_tag="N7B39_002032"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HGU2422021.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRATEVEKE
                     LQRKAQHNENNKETV"
     gene            complement(24043..24129)
                     /gene="leuL"
                     /locus_tag="N7B39_002033"
     CDS             complement(24043..24129)
                     /gene="leuL"
                     /locus_tag="N7B39_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HGU2422022.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
BASE COUNT         5568 a         6594 c         6621 g         5797 t
ORIGIN      
        1 gcaattattg aatgagccag aataagctaa ggttgaaggg gctggaacgc cccttcaacc
       61 ttagcagtag cgtgggatga tttcacaatt agaaagacct gcatgatgag ctagagaaga
      121 ggctagtgac gcaaggcgtc gtgtacgacg cggatcaccg agatgggcat cgccaaccag
      181 actgctaatt agcccatgaa taacaatcag aaaggaccat aacagacccg ataaaatgaa
      241 atataagaga cggtcaacgg gtgaagaaaa agttcaaaaa ttcgctgtgg agcaggaagg
      301 gaattaccga atggaaagcg tagccacacg caacaactga aagcagtttg gcagaaacaa
      361 aaaatccccg gactcgggga tttatgtaca agaggcagcc cttaggatga gggtataaac
      421 gtacaggaaa ggttaaaaat ccgctggcgc tttaaacgtc atactattgc catacgccgg
      481 atgggtaatc gtcaacatct ctgcatgtag caacaaacgc ggtgccatcg ctctcgcttc
      541 tggtgatgca taaaaacgat cgccgagaat cggatgcccc agcgccagca tatgcacacg
      601 caattgatgc gaacgcccgg taatcggttt taacaccact cttgccgtgt tatccgccgc
      661 atactccacc acttcatatt ccgtctgcgc aggtttaccc gtttcgtaac agactttctg
      721 tttcgggcgg tttggccagt cgcaaatcag cggcagatcc accagacctt ctgcggggga
      781 tggatgcccc cagacgcggg tcacatactg ctttttcggc acgcgctcgc ggaactggcg
      841 ttttaactcc cgctccgcgg ctttggtcag cgccactaca atcacgccgc tggtagccat
      901 atccagacga tgcaccgatt ctgcctgcgg ataatctcgc tgaatgcgcg tcatcacgct
      961 gtctttgtgc tcttccagac gacccggcac agacaacaaa ccgctcggct tgttgaccac
     1021 cataatatgg tcatcctgat acaggataac caaccagggt tcctgcggtg gattgtagtt
     1081 ttccatcccc attttcggct ccgttactga tgcgttacaa cgatcaaacg cagggcatcc
     1141 agacgccaac ctgcctgatc caggctttcc attacctgct gacggttgct ctcaatggcg
     1201 gtcagttcgt cgtcacgaat gttcgggttc actgcacgca gagcttccag acgagacagc
     1261 tcggcagaca gtttttcgtc ggcttcgtta cgtgctgcat caatcaatgc acgggcagat
     1321 ttctcgatct gcgcttcacc cagttgaagg atagcgtgaa catcctgctg cacggcgtta
     1381 accagtttgc tgccggtgtg acggttaacc gcgttaagct ggcggttaaa ggtttcaaac
     1441 tctacctgcg ccgccaggtt gttgccgttt ttatccagca gcatacgtac cggcgtcggt
     1501 ggcaggaagc ggttgagctg caactgcttc ggagcctggg cttcaaccac ataaatcagt
     1561 tccaccaaca gcgtacctac cggcaacgct ttgtttttta acagtgaaat cgtgctgcta
     1621 ccggtatcgc cagaaagaat cagatccaga ccgttgcgga tcagcggatg ttcccaggta
     1681 ataaactgcg catcttcacg cgccagcgcc acttcacgat caaaggtgat ggtgatgcca
     1741 tcttccgaca ggccagggaa gtccggcacc agcatatgat cggacggcgt cagcacgatc
     1801 atgttgtcgc cgcgatcgtc ctgattgata ccgataatat cgaacaggtt catggcgaag
     1861 gcgatcaggt tggtatcgtc atcctgctct tcaatgcttt ctgccagtgc ctgggctttt
     1921 tcgccaccgt tggagtggat ttccagcagg cggtcacgac cctgttccag ctgtgctttc
     1981 agcgcttcat gttgctcgcg gcagtttttg atcagatcgt caaagccttc ggtttgatcc
     2041 ggtgaagcca gatagttaat cagatcgttg tatacgctat cgtaaatagt gcgtccggtc
     2101 gggcaggtgt gctcaaatgc atccagacct tcgtgatacc agcgcaccag caccgactga
     2161 gcggttttct ccagataagg cacatggatc tgaatatcgt gcgcttggcc gatacgatcc
     2221 agacgaccaa tacgctgctc cagtagatcc gggttgaatg gcaggtcaaa catcaccatg
     2281 tggctggcga actggaagtt acgtccttca gaaccgattt ctgagcacag cagtacctgt
     2341 gcgccggtgt cttcttcggc aaaccaggcg gcagcgcggt cacgttcgat aatcgacata
     2401 ccttcgtgga acaccgcagc gcgaatacct tcacgttcgc gcagtacttg ctccagttgc
     2461 agcgcagtgg cagctttagc gcagatcacc agcactttct gagagcgatg gctggtcagg
     2521 tagcccatca gccactcaac gcgcggatcg aagttccacc aggtggcgtt atcaccttca
     2581 aattcctgat aaatacgctc cgggtagagc atatcgcggg cgcggtcttc cgcactttta
     2641 cgtgcgccca taatgccgga gactttaata gccgtctgat actgcgtcgg tagcggcagc
     2701 ttaatggtgt gcagctcgcg tttcgggaag cctttcacac cgttacgggt gttacggaac
     2761 agcacgcggc tggtgccgtg gcgatccatc agcatcgaaa ccagctcctg acgggcgctc
     2821 tgggcatctt cgctgtcgct gtttgctgct tgcaacagcg gctcgatatc ctgctcgccg
     2881 atcatctcgc caagcatgtt cagttcgtca ttgctcagtt tgttacctgc cagcagcatg
     2941 gcaacggcgt ccgctactgg acgataattt ttctgctctt caacgaattg cgcgaaatcg
     3001 tggaaacggt tcgggtccag cagacgcaga cgggcgaagt ggctttccat tcccagctgt
     3061 tccggggttg cggtcagcag cagaacgccc ggcacgtgct ctgccagttg ttcaatagcc
     3121 tgatattcgc ggctcggcgc gtcttcgctc cacaccaggt gatgcgcttc atcgaccacc
     3181 agcaggtccc actcggcttc acagagatgt tccagacgct gtttgctacg gcgggcaaaa
     3241 tccagcgagc aaatcaccag ctgttcggtg tcgaacgggt tgtaagcatc gtgctgagct
     3301 tcggcataac gctcatcatc aaacagcgca aagcgcaggt tgaaacggcg cagcatttct
     3361 accagccact gatgctgtaa ggtttccggg acgataatca gcacacgttc agcagcgcca
     3421 gagagcagtt gctgatgaag gatcatcccg gcttcaatgg ttttccctaa acccacttcg
     3481 tcagccagca ggacgcgcgg cgcgtggcgg cggccaacat catgagcgat gttgagctgg
     3541 tgcgggatca ggctggtacg ctgaccgcgc aggccgctgt acggcatacg gaactgttcg
     3601 ctggaatact tacgcgcgcg ataacgcagc gcaaagcggt ccatacggtc aatctgcccg
     3661 gcaaacaggc ggtcctgcgg tttgctgaac accagtttgc tatcaaggaa aacttcacgc
     3721 agggctacgc cggactcttc agtatccagg cgagtaccga tataggtcag caagccattt
     3781 tcttctttta cttcttcgac ttgcatctgc cagccgtcat ggctggtaat ggtatcacca
     3841 gggttgaaca tcacgcgggt cacgggggaa tcactgcgtg cgtacagacg gttttcacca
     3901 gtagatggga aaagtaaagt gacagttcgc gcatccaccg cgacaacggt tccaagtccc
     3961 aattcgcttt ctgtatcgct gatccagcgt tgaccaagtg taaaaggcat atgtgttcgg
     4021 ctctatatct ttaattgcag gcaataacca cccgctaccg tgcttatgag gtagtggtgt
     4081 tattcaggtc caggaatgga aagggcgcta tggtactgga tggcaaagca ttcgtcacgc
     4141 atcaaaatgg tatctggcga actctttttt tgctcaaaat agcccaagtt gcccggtcat
     4201 aagtgtagca aaattatcct caataaaagg gagtattccc tccgccacgg gttgtagctg
     4261 gcgggtcaga tagtgttcgt aatccagtgg tgaacgttgg tagtccagcg gctccgggcc
     4321 gttggtggtc catacgtact taatggtgcc gcggttctga tattgcaagg ggcgaccgcg
     4381 cttttggttt tcttcatcgg caagacgagc ggcgcgtaca tgaggcggaa cattacgctg
     4441 atattcgctc agcggacggc gaaggcgttt acggtaaacc agccgcgcat ccagttcacc
     4501 cgccatcagt ttgtcgatgg tttcgcggaa gtactcctga tatggctcgt tgcggaagat
     4561 acgcaggtaa agctcctgct gaaactgctg ggccagcggc gtccagtccg tacgcacggt
     4621 ttccagccct ttaaacacca tccgctgttt gtcgccctcc tgaatcagcc cggcataacg
     4681 ctttttactg ccggtatcgg ctccgcgaat ggttggcatc agaaaacggc agaaatgggt
     4741 ttcatactcc agttccagtg cgctggtcag ctgttgtttt tgcagcgttt ctgcccacca
     4801 gacgttaacg tgctgcacca gtgcacgacc gattttcgtc gcttcttctt ccgaatgtgc
     4861 gcctttcagc cagacaaacg ttgagtcggt atcgccgtag atcacgtcgt agccctgcgc
     4921 ttcaatcaac gctttggttt gccgcatgat ctgatgacca cgcatggtga tcgacgatac
     4981 cagccgcgga tcgaagaagc ggcaggcggt ggtgccgagc acgccataaa aggcattcat
     5041 gatgattttc agcgcctgcg acagcggttt gttaccctgg cgtttggctt catcgcgtcc
     5101 gtgccagatg ttagtcacaa tctccggcag gcaatgtttt tctcgcgaga accaggcatc
     5161 gagaaagcct tcggtgctgt gctctggatc aggctgcgcc atgccttcca ccagcccgac
     5221 gggatcaatc agaaaggtgc ggatgatcga cgggtacagg cttttgtagt ccagcaccag
     5281 caccgaatca taaagcccag gtcgcgaatc catcacgtag ccgccagggc tggcgtgcgg
     5341 cggcacttcg ccgagattag gcgcgacata accagcgcga tgcatacggg gaaaatagag
     5401 atgaccaaac gctgccaccg acccgccgtg tcgatccacc ggcaggccgt tcaccgttgc
     5461 tcgttcgagc aaaaacggca tgatttcagt tttatggaag atctgcgtca ccagctcgca
     5521 atctttcagg ttataagttg ccagcgcagg tttatcttcg gcgaaacggc ggtcaatttc
     5581 gtccattcga tcccacgggt tatcgataga ttttccttcg cctaatagct cctgagcgac
     5641 agtttccagc gagaatgaag agaaattcca gaacgcggat ttcagcgcct cgataccgtc
     5701 gataattagc cgacctttag cctgggcaaa aaagacgccg tttttaaagc cgtgctcgcg
     5761 ccactccagc tcactgttat cgcgccccag ccgcagcgga atacgataac gctcggcatg
     5821 tttttgcagc attcgcagat caaactgcac cacgttccaa ccgatgatca catcaggatc
     5881 gtagtttgca aaccaggcgt tgagtttttc cagtagctgc gggcggctgg cgacgtactc
     5941 cagttcaaaa tcgagcgcgg aggcgtcgcc attctctggt cccagcatat aaacgatgcg
     6001 ctgcccgcag ccctccaggc cgatgcagta cagctctccg tggcgggtgg tttcaatatc
     6061 aatagaaacc cacttgagcg gcggacgata gtcgggatgc ggtttcagac gggcattaac
     6121 gatagcgcca ttgtgcatat caccctcgac ccacaccggt gaggtgataa accgctccat
     6181 cagatagcgt tctggcgggc gcacatcggc ctcgtagacg gtaacgccac cttcacgcag
     6241 gcgcttttcg taattcatca actggcgatg ggcgcgacag taaaggccat acaccggctg
     6301 gcggtgaaaa tcctttaacg ccagcggcgt caggcgaaag ccttgttcac cccgcaaaat
     6361 atgctgagcg cggggaacct gatcggcggg aataaacgcc acggactctt gcggtgcaag
     6421 cgtaacctgc aacggcccgt tgtccgtcgc cagccagaag gagacctctg ttccttgcgg
     6481 ggtatcccgc cagtgtcggg ttaagataaa acctgcctgc gccacgctga aaatccatca
     6541 aaaaaccagg cttgattata gcctggtttc atttgattgg ctgtggtttt atacagtcat
     6601 tactgcccgt aatatgcctt cgcgccatgc ttacgcagat agtgtttatc cagcagcgtt
     6661 tgctgcatat ccggtaactg cggcgctaac tgacggcaga atatccccat ataagcgacc
     6721 tcttccagca cgatggcgtt atgcaccgca tcttcggcat ttttgcccca tgcaaatggg
     6781 ccgtgagaat ggaccagaac gccgggcatt tgcgctgcat cgataccctg tttttcgaag
     6841 gtttctacga tgacgttacc ggtttcccac tcatattcgc cgttgatttc tgcgtcggtc
     6901 attttgcggg tgcagggaat ggtgccgtag aaatagtcgg cgtgggtggt gccggttgct
     6961 ggaatcgact gacccgcctg cgcccagatg gtggcgtggc gcgagtgcgt gtgcacaatg
     7021 ccgccaatgg acgggaatgc ctgatagagt agtcggtgag ttggcgtatc ggaggagggc
     7081 tttttcgcac cttcaaccac ttcaccggtt tcgatgctga ccacgaccat atcgtcagcg
     7141 gtcatgatgc tgtaatcgac gccggaaggt ttgatcacaa agacgccgcg ctcgcgatca
     7201 acggcgctga cgttgcccca tgtgagcgtg accaggttgt gttttggcag cgccaggttg
     7261 gcttctaata cctgacgttt gagatcttct aacatgttga ctccttcgtg ccggatgcgc
     7321 gttgcttatc cggcctacaa aatcgcagcg tgtaggccag ataagacgcg tcagcgtcgc
     7381 atcaggcgct gaatgccgga tgcgcgttgc ttatccggcc tacaaaattg cagcgtgtag
     7441 gcctgataag acgcgccagc gtcgcatcag gcgttacata ccggatgcgg ctacttagcg
     7501 gcgaaacccg taatacactt cgttccagcg cagcgcgtct ttaaacgctg gcaggcgggt
     7561 gtcgttatca atcaccgtga tttcaatgtc gtgcatctcg gcgaactggc gcatatcgtt
     7621 gaggttcagc gcatggctga agacggtatg gtgcgcgcca ccagcgagga tccacgcttc
     7681 ggaagcagtt ggcagatccg gttgcgcttt ccacagcgca ttcgccaccg gcagtttcgg
     7741 cagggagtgc ggtgttttca ccgtgtcgat gcagttaacc agtagacggt aacgatcgcc
     7801 gagatcaatc aagctggcga caatcgctgg accggtttgg gtattgaaga tcaggcgggc
     7861 aggatcgtcc ttaccaccaa taccgagatg ctgaacgtcg aggatcggtt tctcttctgc
     7921 ggcgatcgac gggcagactt ccagcatatg ggagccgagc accaggtcat tgcctttctc
     7981 gaagtgatag gtgtagtcct ccataaagga ggtgccgccc tgcagaccgg ttgacatcac
     8041 cttcatgatg cgaagcaggg cggcagtttt ccagtcgcct tcgcccgcaa agccgtaacc
     8101 ctgctgcatc agacgctgta cggccagacc aggaagctgt ttcagaccgt gcaaatcttc
     8161 aaaggtggtg gtgaacgcgt ggaagccacc ttgttccagg aaacgtttca tccccagctc
     8221 aatacgcgcc gcttccagca cgttctgtcg tttttcgccg tggatttgtg tggcaggcgt
     8281 catggtgtag cagctttcgt actcatcgac cagcgcgtta acatcgccgt cgctgatgga
     8341 gttcaccacc tgcaccagat cgccaaccgc ccaggtattg acggagaaac cgaacttgat
     8401 ctgtgcggca actttatcgc catcggtgac cgccacttca cgcatgttat cgccaaatcg
     8461 gcagactttc agatgacggg tatcctgttt agataccgcc tgacgcatcc aggagccgat
     8521 acgctcatgg gcttgtttat cctgccagtg accggtaacc acggcatgtt gctgacgcat
     8581 acgcgcgcca atgaagccga actcgcgacc gccgtgcgcg gtctggttca ggttcataaa
     8641 gtccatatcg atgctgtccc acggcagcgc cgcgttgaac tgggtgtgga attgcagcaa
     8701 cggtttgttg agcatggtca ggccgttgat ccacattttg gccggggaga aggtgtgtag
     8761 ccacaccacc agaccagcgc aacgatcgtc gtaattagca tcacggcaaa tagcggtgat
     8821 ttcatccggc gtggtgccca gcggtttcag taccagtttg cagggcagtt tcgcttccgt
     8881 attcagcgct ttcacaacgt gctcggcatg ttgggtgacc tgacgcaggg tttccgggcc
     8941 atacagatgc tggctgccaa ttacaaacca cacttcataa ttatcaaaaa tcgtcattat
     9001 cgtgtcctta tagagtcgga acggcctggg cagcctgtgc cggggcggaa gttggaagat
     9061 agtgttgttc ggcgctcatc gcccattgct gatagcggcg ataaagctgt tcaaagcgtt
     9121 gtgcctgttc gctgcgcggt tgcagggttt tctctaccgc actggccatt ttttgctggg
     9181 ctgatgggat gtctgcgtgc actttcgcgg cgacggcagc aaaaatcgcc gcaccgagcg
     9241 cacagcattg gtcagaggca acaatttgca gcgggcgatt cagcacgtcg cagcaggcct
     9301 gcataatgac ctggtttttc cgcgcgatgc cgcccagcgc catcacgtta ttgacggcga
     9361 tcccctgatc ggtaaagcac tccatgattg cgcgtgcgcc aaaggcggtg gcagcaatca
     9421 aaccgccgaa cagcagcgga gcgtcggtag cgaggttaag atcggtaatc acccctttca
     9481 ggcgttggtt agcgttcggt gtgcggcggc cgttaaacca gtcgagcacc accggcaggt
     9541 gatccagaga cggatttttg gcccatgctt cggtcagcgc cggaagcagt tgtttctggc
     9601 tggcgtcgat ttgctctttt agttccggat gctgggcggc aagctgttcc agcggccagc
     9661 cgagtacgcg accgaaccag gcgtagatat caccaaacgc cgattggcct gcttccagac
     9721 cgataaatcc aggcaccacg ctgccatcaa cctgaccgca aatacctttc actgcccgct
     9781 cgccaacgct ctgtttgtcg gcaatcagaa tgtcgcaggt ggaagtaccg ataactttta
     9841 ccagtgcgtt aggctgtgcg cctgcaccaa ctgcgcccat atggcagtca aacgcgccgc
     9901 cggaaatcac cacgctttca ggcaggccga gacgctgcgc ccattccggg cataaggtgc
     9961 ccaccggaat atcggcagtc caggtatcag tgaacagcgg ggaaggcaaa tggcgattga
    10021 ggatcggatc cagctcatca aagaaactgg ctggcggcag gccgccccag ctttcgtgcc
    10081 atagcgactt atgcccggcg ctgcaacgtc cgcgacgaat atcctgcggg cgcgtggtac
    10141 cggaaagcag agctggcacc cagtcgcaca gctcaatcca cgatgcggca gattgcgcca
    10201 cggcgctgtc ctggcgagtc acatgcagga tttttgccca gaaccattcg ctggaataaa
    10261 taccgccaat atagcgggag tagtcaacat tgcccggcgc gtggcacaaa cgggtaatct
    10321 cttccgcttc ttcaaccgca gtgtggtctt tccacaatac gaacatcgcg ttcgggtttt
    10381 cggcaaactc cgggcgcagc gccagcacgt taccgtcggc atcaatcggt gcgggcgtcg
    10441 agccggtact gtcaacgcca atcccgacca cagctgcgcg ctgttcgacg ctaagttctg
    10501 caagcacggt tttcagtgcc gcttccattg actcaatgta gtcacgcgga tgatgacgga
    10561 actggttatt cggggcatca caaaattgcc ctttttgcca acggggatac cactctacgc
    10621 tggtggcgat ttcttcaccg gtggtgcagt ccaccgccaa agctcgcaca gaatcactgc
    10681 caaaatcgag gccaattgca atcgccatcg tttcactcca tccaaaaaaa cgggtatgga
    10741 gaaacagtag agagttgcga taaaaagcgt caggtaggat ccgctaatct tatggataaa
    10801 aatgctatgg catagcaaag tgtgacgccg tgcaaattat caatgtggac ttttctgccg
    10861 tgattataga cacttttgtt acgcgttttt gtcatggctt tggtcccgct ttgttacaga
    10921 atgcttttaa taagcggggt taccggttgg gttagcgaga agagccagta aaagacgcag
    10981 tgacggcaat atctgatgca atatggacaa ttggtttctt ctctgaatgg cgggagtatg
    11041 aaaagtatgg ctgaagcgca aaatgatccc ctgctgccgg gatactcgtt taacgcccat
    11101 ctggtggcgg gtttaacgcc gattgaggcc aatggttatc tcgatttttt tatcgaccga
    11161 ccgctgggaa tgaaaggtta tattctcaat ctcaccattc gcggtcaggg ggtggtgaaa
    11221 aatcagggac gagaatttgt ctgccgaccg ggtgatattt tgctgttccc gccaggagag
    11281 attcatcact acggtcgtca tccggaggct cgcgaatggt atcaccagtg ggtttacttt
    11341 cgtccgcgcg cctactggca tgaatggctt aactggccgt caatatttgc caatacgggt
    11401 ttctttcgcc cggatgaagc gcaccagccg catttcagcg acctgtttgg gcaaatcatt
    11461 aacgccgggc aaggggaagg gcgctattcg gagctgctgg cgataaatct gcttgagcaa
    11521 ttgttactgc ggcgcatgga agcgattaac gagtcgctcc atccaccgat ggataatcgg
    11581 gtacgcgagg cttgtcagta catcagcgat cacctggcag acagcaattt tgatatcgcc
    11641 agcgtcgcac agcatgtttg cctgtcgccg tcgcgtctgt cacatctttt ccgccagcag
    11701 ttagggatta gcgtcttaag ctggcgcgag gaccaacgca tcagccaggc gaagctgctt
    11761 ttgagcacta cccggatgcc tatcgccacc gtcggtcgca atgttggttt tgacgatcaa
    11821 ctctatttct cgcgagtatt taaaaaatgc accggggcca gcccgagcga gttccgtgcc
    11881 ggttgtgaag aaaaagtgaa tgatgtagcc gtcaagttgt cataattggt aacgaatcag
    11941 acaattgacg gcttgacgga gtagcatagg gtttgcagaa tccctgcttc gtccatttga
    12001 caggcacatt atgcaagcat tgctggaaca ctttattacc caatccaccg tgtattcatt
    12061 gatggcggtg gtgttggtgg cctttctgga gtcgctggcg ctggtcggtt tgattctacc
    12121 cggtacggtg ctgatggcgg ggctgggagc gctgattggc agcggcgagt taagtttctg
    12181 gcacgcctgg ctggcaggga tcgtcggttg tctgctgggc gactggatct ccttctggct
    12241 gggctggcgt tttaaaaagc cattgcatcg ttggtcattt ctgaagaaga acaaagcatt
    12301 actcgataaa actgaacatg cgttgcatca acacagcatg ttcaccattc tggtcggtcg
    12361 ttttgttggc ccgacgcgtc cgctggtgcc aatggtggcg ggaatgctgg atctcccggt
    12421 ggcaaaattt atcacgccga atattatcgg ctgcctgctg tggccaccgt tttacttcct
    12481 gccggggatt ctggcgggtg cggcgatcga tattccagcc ggaatgcaga gcggtgagtt
    12541 taaatggttg ctgctggcga cggcggtatt tttgtgggtt ggtggctggc tgtgctggcg
    12601 gttatggcgc agcggcaaag caaccgatcg tttgagtcat tatttgtccc gcggtcgttt
    12661 gttgtggctg acgccgttga tttctgccat cggcgtggtg gcgctggtgg tgttagttcg
    12721 ccatccgtta atgccggtgt atatcgatat tttgcgtaaa gtggttggag tttagggcat
    12781 agtcttgtgt ggggcgcttg atgcggacaa aacgcacccg taggtcggat aaggcgttca
    12841 cgccgcatcc gacagtgcat attaacctgt aattcccaac agcgccgaag cactcgcctt
    12901 accgctcaac aactcattgg tcataccctg ccaggcgatt cgcccgtcgg cgactactac
    12961 cgagcgcgtg gcaatccgcg ccgcatcttc cacactgtgc gacaccatca atagcgtcat
    13021 tttttgctgc tggcagctag tgctcaccag cgtcaacatc tcctgacgta gcgccggatc
    13081 gagcgcagag aacggttcat cgagcaataa aatcggctgt tcgcgtacca gacaacgcgc
    13141 taacgccacg cgctgtcgct gaccgccgga aagctcgccc ggtaaccgcg ccattaaatt
    13201 atcaatcccc atctgacggg caatcgcgtg cattttctcc tgctgtaccg cgttcagctt
    13261 caatcctgga ttaagcccca gcccgatgtt ctgcgcgacc gtcaggtggc tgaacaggtt
    13321 gttctcctga aacagcatcg acaccggacg gcgtgacggc ggcgtcgtgg tgtgatcaac
    13381 gccgtcgata gtcagcgcac cgctggctgg tgtcagaaaa ccggcgatca agttcagcag
    13441 ggtactttta cccgcgccgc ttggcccgag gatcgccacc tgctcgccgc gttctaccgt
    13501 taagctaaaa cgcatcggca aatggtgata aagccaggtg atatcagtca gttttaacat
    13561 ttcgccccgg tattttttca atcacgctaa acagcagaaa acagagcagc agcagaatta
    13621 acgcggtgac cgcgccgtcc tggctgcgat aggagccaat ttgctggtag agataaaacg
    13681 gcagggtgcg gaaatcatcg ttaccgaaca acgccaccac gccaaaatca ccaatcgaca
    13741 gcacgcaggc aaaggccagc gcctgcgcca gcgggcgttt cagggcgcgc agctccacta
    13801 cttttaagcg tgaccagcct tcaatcccca gcgactgaca taacatgctg tagcgggcgg
    13861 tgatatcgcg catcgggttt tccagcactt tcagggcgta ggggatcgcc attaacgcat
    13921 tggtgaaaat cacaatgccg tcagcagatt gtggcaggcc gatagtgttg ttgagcagta
    13981 aaaagaagcc cgttgccagc acaatccccg gcatagcgag gatcaacatg ccgctcatct
    14041 ccagcgcctg gcccgccagc attttttgcc gcgcccgcag ttcgcgactg ctccatagca
    14101 gcatcatggt cagcactacg cacaatacac ctgccgccag cgcaatacgc aacgaggtcc
    14161 acagcgcctg ccacagcacc ggttgtgcca gcacttccgg caactggcga tttaccccat
    14221 cgacgatcac cgccagcaac ggtggcagca acagcagcag cgccagcaca attaacatcg
    14281 tgtcgcaaat gcggctatgc agacgatcgt ccgggtcgcg ccagccttgc agcagcgtgg
    14341 tgccgggcgc aatggcctta ctcagtcgct gactcaacag caccagccca aggcaacaca
    14401 ccatctggat cagcgccagc atcgccgcgc gggcaggatc gtagtcgtaa ctcagcgcct
    14461 gatagattgc cagctcgata gtggttgcct gcggaccgcc gcccagcgat aacacggtgg
    14521 cgaagctggc gaaacagagc ataaagataa gcgcggtaac cggcgggatt tgtcgccgta
    14581 accacggcca ttcgacgaag cggaaaaaat gccagccgcg catcccaagc tgggcggcaa
    14641 gctgacgttg ttcgccggga atgttttcca gtgcctggag taataagcgg ctcgccatcg
    14701 gcagattaaa aaacacatgg gccagcaaaa tgccttgcag gccgtagggc gaaaaggtcc
    14761 actccagacc gagcgattgg cagagcgatg ccagccagcc ctggcgacca tagacgctaa
    14821 gaatgccgaa aacagcaacc agcaccggga ggatcaaggt cattgcgcac agacgcaaca
    14881 gcgccagccg acccggaaag cgcctgcgat agagcgcgcg ggcgaggaat atcgcgggta
    14941 cgacagagag tagcgccgag agaaacgcct gccagaagga gaagcgcacc acatgccaca
    15001 ggtagctgtc ctgccagact gccgaccagt caccctgcgg cgcgttccac cacagggcga
    15061 gaaacgccgc cagcgcaacc gctaccacca gcgtggcggc gcttacacct ggaattaacc
    15121 agccgggaat taacggctga cggcgcgttg ccattcgcta atccatgcct gacgttgtgc
    15181 cgccacttcg gctggcgtga actccagcgt ggttgcgggt ttggtcaatt tttcaaaacc
    15241 ggcaggcagc gtgacgtttg ccaccggata catccagttg ccggttggga tcgcattctg
    15301 gaaagccgga gaaaccataa actggaggaa tttttgcgcc agctccggct gcttgctggc
    15361 agcggtgcga gcggcgactt ccacttgcag atagtgacct tcgctgaagt tcgcggcggc
    15421 gtagttatct ttcttctctt cgagaatgtg ataagccgga gaggtggtgt aactcagtac
    15481 cagatcgctt tcacctttta aaaacaggcc gtaggcttcg ctccagcctt tggtgaccgt
    15541 gaccgttttc ttcgccagtt tctgccaggc ttgtggggcg tcatcgccat agactttttg
    15601 catccatagc aacagaccca gccccggtgt actggtgcgc ggatcctgat aaatcacccg
    15661 ccagttttga tcgctctcaa ccagttcttt caggctttgt ggcgggtttt tcagtttgtt
    15721 cttgtcataa acgaaggcga agtagccata atcaaacggt acgaaaatgt cattattcca
    15781 gccgccggga acgttaacgg catccgctgc cacaccgctt ttggcaaaca gtccggtttt
    15841 actggcggcg tctaacagat tgttatccag ccccagcacc acatcggctt tactgttttt
    15901 gccttccatc cgtagacggt tgagaagcga aacgccatct tccagcgcca ccagtttcag
    15961 ttcgcaatta cagtcggctt caaaggcttt tttaaccacc ggaccaggcc cccagtcggc
    16021 ggcgaaggaa tcgtaggtat aaacagtcag aacgggttta gcgaaaacgg gcgctgtgca
    16081 cagcaacagc aggggaagac atttttttaa cactttgcac ctcaaaaaag agtggcaaag
    16141 gacttgagaa ggagcctcaa atcccttcgc cggcgttatc cggatcaggt tcgacgggta
    16201 ttttctcagc gcacgcgtcc gcgtggcacc ccgttgagaa cggcgttagt gtagtgattt
    16261 tgttatcaac cagcaatcca tagagaaggc gtattaaaaa agccaatatt cgttcagcta
    16321 tcaagagcgt atttgcatag ctggagagcg ttgatccgaa agaatccctt cgctacagtc
    16381 aaaataggta acgtaaccgt ttattgcgtg aatgagcgat ttttgacagt attcttattc
    16441 aggacgctta ttctgagttt tttattcaga gtggagctat cagcatgaaa gtaagaaacc
    16501 cagaacagat tagtatccct gctagtaata ccactaaaga tccaggcctt actaattctc
    16561 agattatcag gatggctaac ttggtcaaaa agaccgagaa tatgaatatc tttgaagagc
    16621 tatgggagac gttacgcaat ctcttccagt cagataaaca ttcccagact gcggcaaggc
    16681 aaattctgaa ggatgcattt tatttccaga atagtgatga ctactcaaaa tactttaccg
    16741 gggctgtcga cgggaaggcg cgtgataagt taactcactg gctgataaaa tttaatgaac
    16801 tcaaagaata cgcaaaagat ccggaaaata tggccgcgaa ggcctcactc tcgccagaag
    16861 gtacgttatg cgtcagtttt tttattggcg atgaggcgat atttactctg gagttgcagc
    16921 tgaaaaagag caccaggacc ggggggattg atctgagtaa tgcttacttt aacggggttg
    16981 tcatttgtgg tattgactgt ctcgaggttg acctgagcaa tgctgaaacg aacaatagcc
    17041 gttggtatga ctgacctgcg cgaggttaac ctgaaaaagc tgacctttcc ggccagaaca
    17101 aggctagact gcgccgttat tgattaactc agaatatgaa agaggggctg gaatgcccct
    17161 tctttaatct tatttgcgac gctaatcaga cgatgcataa aacagagaaa atataaaagt
    17221 aatgtatttt atcattccgt ctgaatataa aaaatgcgct catgaccgcc aaaaggcgca
    17281 agctactctg gatccggcgg cgcaaaccac gctgatttaa aatcaaacca gccgagggtg
    17341 ttcatgcgca ggccgcgcat actgcgttgt ccctgaatga tcagccagtg gtggataagt
    17401 ggcaccattg ctttgctggc gaccagttgt tggcaccagt tcgccaggtt catttcgcca
    17461 ttgcgccagc gggcggcgtc gacttgccag tcgattggaa tacagtgttg gagcagcggt
    17521 acctcgcaca ggtgcgcgaa cagcgaaaaa tccagcggca gcgtaaagtt ggcgctgtta
    17581 agccagatat cgctctcgat ctctccttca tgccactgat cgtagctgat ctctttgatt
    17641 tccagtgtga cctggtggct tgccagaatc tgctgcatga tcccggcaat cacccgatgc
    17701 tcactgtgat cctgataaaa ggtcagggtg aggctttcca gaccagccgg tttttcgctc
    17761 gttatggtgc gggcatgatg ccaacggggg agcagtccat aagccgggaa ccacagttgc
    17821 tggtactgtt cctcagcgaa atagaccaga ttagttggag aaagcacata gcttacccag
    17881 tccctgactt gctgattcgc cccgcgatgg gtgcggctat cgaacagtaa atagtagcaa
    17941 ccttcctcca ggcggctttc aatctctttt tcctcgccct gtggaccttt tagcatcagc
    18001 cctccggctg gctcgtcggc aatttccggc agaacccaga cgttaacttc atcgattaat
    18061 gcccggtaac cgaagaagtc atcgaatgcc tgaattttca gttgattggt gctgttgcga
    18121 atcaccgcat acggaccggt gccgatggga tggctggcaa agttactgag ggtttcccat
    18181 tcgcgcggca ggatcatcgc cggaacttgt cccagcagta acggcaacca gcgatccggc
    18241 tgcgtgaggt ggatatccag cgtccagggc gtcggcgaca caatgtcagc aatatgcgaa
    18301 tagagcggca gcgtattgat tcgttttaaa gaggcgatca cgtcgtccat ttccagttca
    18361 cgaccatgat gaaaatggac tcctggacgc aaaaagaaac gccagtgaag cggtgatatt
    18421 tgctgccagt ggtgggcgat gtctgcttcc agttccccat tttcctcatt tatgcgcgtt
    18481 agcgaactga agatttgccg ggcgatatgg gtttcggaac ggcgcaatgc gctgccaggt
    18541 agcagattac gcaacggacg atagtagagc acgcgcagga tgtgccgccc ctggcggaag
    18601 ctgcggccca gatgagaaac cagcatttgc ctcacagtcg ctttgtcgcc aactaactgc
    18661 actagttggt cgatacggtc ctgctccagc aggtcttccg cccgctgttg ctgaagcgcc
    18721 agcccggtat agaggaatgt cagacgcgag cgtttaccac gcccgacttc cgcttcccac
    18781 gtcagccagc cgcgatcctg catggtgttg agcagggtac gcatatgacg acgcgagcag
    18841 ctcaataacg ctgccagttc gttgagcgtt gtgtcctgcg atttaccctc gcagcattgc
    18901 cacaggcgga tgaactgttg ttgcagacga gcagatggca taaaagggga actcctgcgc
    18961 aaaagacagc aattttattt tccctatatt aagtcaataa ttcctaacga tgaagcaagg
    19021 aggtgcccca ttcgtcagtt ttatcagcac tattttaccg cgacagcgaa gttgtgctgg
    19081 ttgcgttggt taagcgtccc acaacgatta accatgcttg aagaactgat gcagtgggag
    19141 gggagtcatt ctgactactg atttgcggac atcatgtgtg actgagtatt ggtgttaatc
    19201 gccacgccag cagtgattat ctgctggctt ttttcttttt ggcgattgag tatcggacgg
    19261 tgtacaaact aacgcataaa cgcgggttgc ttcgcttcat aagccgcaat ggcgtcgtcg
    19321 tgctgcaagg taagcccaat gctgtccaga ccgttcatca tgcagtggcg gcggaaggca
    19381 tcaatggtaa agcgataggt tttctcaccc gctttcacct cttgcgcttc caggtctaca
    19441 tcaaaatgaa tccccggatt ggctttcacc agcgcaaaca gctcatccac ttctgcgtcg
    19501 cttaatttca ccggcagcag ctggttgtta aagctattgc cgtagaagat gtcagcaaaa
    19561 ctcggcgcaa tcaccacctt aaaaccgtag tcggtcaatg cccagggcgc gtgctcacgc
    19621 gaggagccac agccgaagtt ttctcgtgcc agcaaaatgg aagcgccctg atactgcgag
    19681 aagttcagca cgaagtccgg gtttggctgt tggccttttt catccagaaa acgccagtcg
    19741 ttaaacagat gcgcgccaaa accagtacgg gtcactttct gcaaaaactg tttcgggatg
    19801 attgcatcgg tatcgacatt ggcggcatcc agcggaacca ccaggcctgt gtgtttgata
    19861 aatttctctg ccatggtgtg ctccttattt aatgttgcga atgtcggcga aatggccggt
    19921 cacagccgcg gcggcagcca ttgccgggct gaccagatgc gtgcgcccgc cgcgcccctg
    19981 acggccttca aagttacggt tgctggtgga ggcacaacgt tcgcccggat tcagacggtc
    20041 gttgttcatc gccagacaca ttgagcagcc aggcaagcgc cattcaaaac cggcttcaat
    20101 aaagatttta tccagacctt ctgcttccgc ctgcgctttt accggaccag agccgggaac
    20161 caccagcgcc tgtacgcctg gcgcgacttt ccgccctttg gcgatttccg ccgccgcgcg
    20221 taaatcttca atacgtgagt tggtgcagga accgataaac actttgtcga tagccacttc
    20281 ggtcagcaga atacccggtt tcagccccat ataggccaac gctttttctg ccgacgcgcg
    20341 ttcaaccgga tcggcaaacg aagccggatc gggaatattg tcgttcacgg aaatcacctg
    20401 gcctgggtta gttccccagg tgacctgcgg tgaaatctct tctgcctgca aagtgacaac
    20461 ggtatcgaaa gttgcgcctt cgtcggtttg cagggttttc cagtaggcaa cggcgtcgtc
    20521 gaaatctttg cctttcggcg catgcagacg gcctttgaca tagttaaagg tggtttcgtc
    20581 aggtgcaacc agaccggctt tcgcgcccat ttcgattgcc atattgcaca gggtcatccg
    20641 accttccatg cttaaatcac ggattgcttc gccgcaaaac tccaccacat gcccggtgcc
    20701 gcctgcgcta ccggttttac cgataattgc caacacgata tcttttgcgg taatgcccgg
    20761 cgcggctttg ccctggactt caattttcat ggtcttcgca cggccctgtt tcagggtttg
    20821 cgttgccagt acgtgttcaa cttcggaagt gccgatacca aaggccagtg cgccaaacgc
    20881 gccgtgggtg gcggtatgcg agtcgccgca gacaatggtc atccccggca aggtcacgcc
    20941 ctgttccggc cccattacgt ggacgatccc ttgatagggg tgattcaggt catacagttc
    21001 gacgccaaat tctttgcagt ttttgatcag ctcctgcatc tggatgcgcg ccatttcacc
    21061 gcaggcatta atgtctttag tctgggtaga gacgttgtga tccatggtgg cgaaggtttt
    21121 gcccggctga cgtaccgggc gaccgtgggc gcgcagacca tcgaacgcct gcggtgaggt
    21181 cacttcatgc accaggtggc ggtcgatata taacagcggg gtttcatttt cggcttcgta
    21241 cactacgtga gcgtcgaata atttttcgta taacgtctta gccatgatta caccccttct
    21301 gccacatagc gggcaatgat atcgcccatt tcatcggtac taacggcggc agcgccacgg
    21361 gctaaatccc cggtgcgaat gccttcttct aatgcgcggt taatggcgcg ttcaatggcg
    21421 gaagccgcat catcggcatc caggctgtaa cgcagcagca gtgccagcga cagaatttgc
    21481 gcaatcgggt tggcgatgtt tttacctgcg atatctggtg ccgagccgcc cgccggttca
    21541 tacagtccaa aaccttgctc gttcaggctg gcggaaggca acatccccat cgagccagtg
    21601 atcattgcgc actcgtcaga cagaatgtcg ccaaacaggt tggagcacag cagaacgtca
    21661 aactgtgatg gatctttaat cagctgcatg gtggcgttgt cgatgtacat atgcgccagt
    21721 tcgatatccg ggtattccgt ggcgatctcg ttgacgattt cacgccataa aatagaggat
    21781 tgcagcacgt tggctttatc gatcgacgtc actttatggc gacgtttacg agcagattca
    21841 aacgcgatgc gggcaatacg ctcgatctca aaacggtgat acacctcggt atcaaacgct
    21901 ttttcatatt gtccgctacc ttcgcggcct tttggctgac cgaaatagat gccgccggtc
    21961 agttcgcgca cgcacaggat gtcgaagccg tttgcggcaa tgtctgcacg cagcggacag
    22021 aatgcttcca gcccctgata cagttttgcc ggacgcaggt tgctgaataa tttgaagtgc
    22081 ttacgcaaag gcaacagcgc gccgcgttct ggttgctggt ccggtggtaa atgttcccat
    22141 ttcgggccgc ctaccgagcc aaacagcacg gcatcggctt gctcacaacc ttcaaccgtc
    22201 gcaggcggca gtggttgccc gtggttatca atggctgcgc cgcctacatc gtaatggctg
    22261 gtagtgatgc gcatcgcaaa gcggttgcgc acggcatcca gcactttcag cgcctgggtc
    22321 atcacttccg gaccaatgcc atcccccggc aatacggcaa tatggtaatt cttcgacatc
    22381 acacggtttc cttgttgttt tcgttgtgtt gagctttgcg ttgcaactct ttttcgactt
    22441 ctgtggcacg ccagatattg ttaagtacgt gcaccatggc tttggcggag gactcgacaa
    22501 tatcggtggc caggccgacg ccgtggaagc ggcgaccgtt gtagttggcg acaatatcca
    22561 cctgacccag cgcatcttta ccgtgacctt tggcggtcag gctgtatttc accagttcga
    22621 cgttatagtc agtgatgcgg ttaatcgcct gatagacggc atcgaccgga ccgttaccgt
    22681 tggcggcttc tgctttgact tcttcgccgc aggccagttt gacggcggcg gtggcgatat
    22741 cgttagagcc agactgcacg ctgaagtaat ccagacggaa atgctccggc tcttcttgct
    22801 gcttaccgat gaaggccagc gcctccagat cgtaatcaaa cacctgacct tttttgtccg
    22861 ccagcttgag gaaggcgtcg tacaaattgt ctaaattata ttcactttct ttatacccca
    22921 tctcatccat gcgatgtttc accgccgcac gccccgaacg agaggtcaga ttcagctgga
    22981 tttggttcag accaatagat tctggtgtca tgatttcgta gttttcgcgg tttttcagca
    23041 cgccatcctg gtggataccg gaggagtgtg cgaatgcgcc gctgccaaca atggctttgt
    23101 ttgccgggat cggcatatta caaatctggc taactaactg gctggtgcgc catatctcct
    23161 ggtgattaat ggcggtgtgg acgttgagaa tatccttacg aactttaatc gccatgatga
    23221 cttcttccag ggaacagtta ccggcacgct cgccgatccc gttcattgca ccttccacct
    23281 ggcgtgcacc ggcatgtacc gccgccagtg agttgccgac cgccaggccc aaatcgtcgt
    23341 gggtatgtac ggaaataatg gctttgtcga tgttaggcac gcgttcatac aggccgctaa
    23401 tgattccggc gaactcaaac ggcatggtgt agcccacggt gtccggaatg ttgatggtgg
    23461 tggcaccggc attaatcgcc gcttcgacca ctcgcgccag atcggcaatg ggtgtacgcc
    23521 cggcatcttc gcaagaaaat tcaacatcat cggtgtaatt acgggcgcgt ttcaccatat
    23581 agatagcgcg ttcgatcacc tcgtccagcg tgctgcgcag cttggtggcg atgtgcattg
    23641 gcgaagtggc aataaaggta tgaatacgga aggcttcggc gactttcagg gattcggccg
    23701 ccacgtcgat atctttctcc acgcagcgag ctaacgcgca tacacggctg tttttaacct
    23761 ggcgagcgat ggtttgcact gattcaaaat cacccggcga agagacgggg aacccgactt
    23821 ccatcacgtc aacacccata cgctcaaggg ccagcgcaat ttgcagtttt tctttcacac
    23881 tcaagcttgc ctgtaacgcc tgttcaccgt cgcgcaatgt ggtatcgaaa ataatgactt
    23941 gctggctcat ggtttgggtc cttgtctctt ttagagcgcc tcgcttcggg cataaaaaaa
    24001 accgcgcaat ggcgcgggtt ttttgtttga ctgcgtgctg gcttaatgct ggatgccgct
    24061 cactcgtcta ccgcgcaaag aagatgcgtt tagtagtagt agaccgataa agcgaacgat
    24121 gtgagtcatt aaatcagctc cagatgaatg cgatatgctt ttagagttac tggatacaaa
    24181 aacggatgtc aaccctgacg caataaaaac gtcccgccag catgagttct gcatccgtaa
    24241 gattagctaa ttgtgctgct gtggttaaaa caagcgatat taatttctgc ttaactaccg
    24301 atgcttttca tcggttgaca tatttcagaa taaatttttg catctaatcc acgaggaaaa
    24361 aggggacaaa atgcacgcgt tgcaaaacct atcctgatga tttgtattga attatatgtt
    24421 ttgcggattt tttgatattg atttggtgaa tattattgat caattaatgt taagaattaa
    24481 tgcattaaat atataaataa attattaaaa agcacattta atccattttg tggatgattg
    24541 agtattcgcg gtagttatga ttagattgtt tacgcaacaa
//
LOCUS       DBFIFI010000053        24254 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.053, whole genome shotgun sequence.
ACCESSION   DBFIFI010000053 DBFIFI010000000
VERSION     DBFIFI010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24254
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.053"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..754
                     /locus_tag="N7B39_002034"
     CDS             <1..754
                     /locus_tag="N7B39_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HGU2422023.1"
                     /translation="IWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIF
                     LKPMDIHSYHCEGLVWEQYWMEFTPTSMMDIPIGQQSVIYNGEIYNQELTEVAELITS
                     PEAIKNNLAVAFLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHASLQQRWSVADMA
                     ATIPCSEAWLRRLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSF
                     HFSKAFKHKFGYAPSAVLKNTDQHPTDASPHN"
     gene            complement(751..2073)
                     /locus_tag="N7B39_002035"
     CDS             complement(751..2073)
                     /locus_tag="N7B39_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HGU2422024.1"
                     /translation="MTKFSVVVAGGGSTFTPGIVLMLLANQDRFPLRALKFYDNDGSR
                     QEVIAEACKVILKEKAPDIAFSYTTDPEVAFSDVDFVMAHIRVGKYPMRELDEKIPLR
                     HGVVGQETCGPGGIAYGMRSIGGVLELVDYMEKYSPNAWMLNYSNPAAIVAEATRRLR
                     PNAKILNICDMPIGIESRMAQIVGLQDRKQMRVRYYGLNHFGWWTSIEDLQGNDLMHQ
                     LRQYVSKHGYVPPQQDTHTEASWNDTYAKARDVQALDPDTLPNTYLKYYLFPDYVVQH
                     SNPEHTRANEVMEHREKQVFDACRAITAAGNSAAGKLEIDEHASYIVDLAAAIAFNTQ
                     ERMLLIVPNNGAIHNFDDEAMVEIPCLVGHNGPEPLVVGDIPQFQKGLMSQQVAVEKL
                     VVDAWEQRSYQHLWQAITLSKTVPSASVAKAILDELLEANKAYWPELR"
     gene            complement(2073..3689)
                     /locus_tag="N7B39_002036"
     CDS             complement(2073..3689)
                     /locus_tag="N7B39_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGU2422025.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            3985..4701
                     /gene="yidP"
                     /locus_tag="N7B39_002037"
     CDS             3985..4701
                     /gene="yidP"
                     /locus_tag="N7B39_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGU2422026.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPDKS"
     gene            complement(4698..6359)
                     /gene="yidE"
                     /locus_tag="N7B39_002038"
     CDS             complement(4698..6359)
                     /gene="yidE"
                     /locus_tag="N7B39_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HGU2422027.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(6555..6983)
                     /gene="ibpB"
                     /locus_tag="N7B39_002039"
     CDS             complement(6555..6983)
                     /gene="ibpB"
                     /locus_tag="N7B39_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HGU2422028.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(7095..7508)
                     /gene="ibpA"
                     /locus_tag="N7B39_002040"
     CDS             complement(7095..7508)
                     /gene="ibpA"
                     /locus_tag="N7B39_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HGU2422029.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(7502..7574)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            7814..8146
                     /gene="yidQ"
                     /locus_tag="N7B39_002041"
     CDS             7814..8146
                     /gene="yidQ"
                     /locus_tag="N7B39_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HGU2422030.1"
                     /translation="MIRNVLLAFMICSGMALLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(8148..9362)
                     /gene="yidR"
                     /locus_tag="N7B39_002042"
     CDS             complement(8148..9362)
                     /gene="yidR"
                     /locus_tag="N7B39_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HGU2422031.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRVSQGAHVGVVTVHPKSEKYVFIHGPENPHETWHYDFHHRRGVIVE
                     GGKMNNLDAMDITVPYTSGALRGGSHVHVFSPNGEMVSFTYNDHVMHQFDSALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGIVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLGNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            9463..10527
                     /gene="cbrA"
                     /locus_tag="N7B39_002043"
     CDS             9463..10527
                     /gene="cbrA"
                     /locus_tag="N7B39_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HGU2422032.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQHGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRYLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRSVLLKQPEKINAAYWHATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGMAHIPQLKD"
     gene            complement(10524..10637)
                     /locus_tag="N7B39_002044"
                     /pseudo
     CDS             complement(10524..10637)
                     /locus_tag="N7B39_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418146.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10773..11429
                     /gene="yidX"
                     /locus_tag="N7B39_002045"
     CDS             10773..11429
                     /gene="yidX"
                     /locus_tag="N7B39_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidX"
                     /protein_id="HGU2422033.1"
                     /translation="MKLNFKGFFKAASLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVANDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(11475..12287)
                     /gene="yidA"
                     /locus_tag="N7B39_002046"
     CDS             complement(11475..12287)
                     /gene="yidA"
                     /locus_tag="N7B39_002046"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HGU2422034.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGNTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(12402..12800)
                     /gene="yidB"
                     /locus_tag="N7B39_002047"
     CDS             complement(12402..12800)
                     /gene="yidB"
                     /locus_tag="N7B39_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HGU2422035.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQPVSGEQLESALGTNAVSDLGQKLGVDTSTASCLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            12918..13016
                     /locus_tag="N7B39_002048"
                     /pseudo
     CDS             12918..13016
                     /locus_tag="N7B39_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13040..15454)
                     /gene="gyrB"
                     /locus_tag="N7B39_002049"
     CDS             complement(13040..15454)
                     /gene="gyrB"
                     /locus_tag="N7B39_002049"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HGU2422036.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(15483..16556)
                     /gene="recF"
                     /locus_tag="N7B39_002050"
     CDS             complement(15483..16556)
                     /gene="recF"
                     /locus_tag="N7B39_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HGU2422037.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(16556..17656)
                     /gene="dnaN"
                     /locus_tag="N7B39_002051"
     CDS             complement(16556..17656)
                     /gene="dnaN"
                     /locus_tag="N7B39_002051"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HGU2422038.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(17661..19064)
                     /gene="dnaA"
                     /locus_tag="N7B39_002052"
     CDS             complement(17661..19064)
                     /gene="dnaA"
                     /locus_tag="N7B39_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HGU2422039.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKSVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            19361..19441
                     /locus_tag="N7B39_002053"
     CDS             19361..19441
                     /locus_tag="N7B39_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2422040.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            19671..19811
                     /gene="rpmH"
                     /locus_tag="N7B39_002054"
     CDS             19671..19811
                     /gene="rpmH"
                     /locus_tag="N7B39_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HGU2422041.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            19828..20187
                     /gene="rnpA"
                     /locus_tag="N7B39_002055"
     CDS             19828..20187
                     /gene="rnpA"
                     /locus_tag="N7B39_002055"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HGU2422042.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVANLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            20151..20408
                     /gene="yidD"
                     /locus_tag="N7B39_002056"
     CDS             20151..20408
                     /gene="yidD"
                     /locus_tag="N7B39_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HGU2422043.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFNTREH"
     gene            20411..22057
                     /gene="yidC"
                     /locus_tag="N7B39_002057"
     CDS             20411..22057
                     /gene="yidC"
                     /locus_tag="N7B39_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HGU2422044.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            22163..23527
                     /gene="mnmE"
                     /locus_tag="N7B39_002058"
     CDS             22163..23527
                     /gene="mnmE"
                     /locus_tag="N7B39_002058"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HGU2422045.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSAKTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            complement(23677..24254)
                     /locus_tag="N7B39_002059"
                     /pseudo
     CDS             complement(23677..24254)
                     /locus_tag="N7B39_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545037.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="toxin"
BASE COUNT         5913 a         6414 c         6132 g         5795 t
ORIGIN      
        1 gatctggcca cgggacaaga agaaaccgct tatcgccaac tcatgggttg cggtgtatac
       61 cgtacaagga tgtgggaaga ttcttttaaa gaatggcgaa caaataacac tgcatggcaa
      121 ctgtattata tttttaaagc caatggatat tcactcttat cactgtgaag gtttagtctg
      181 ggaacaatac tggatggaat ttacccccac cagtatgatg gatattccca tcggtcagca
      241 aagcgttatt tacaatggcg aaatttataa tcaggaactc accgaagttg ctgagttaat
      301 aacttcacca gaagcaataa aaaataatct ggcagtcgct tttctgacga aaattattta
      361 tcagtggatt tgtcttatgt acgcagacgg taaaaaagat ccacaacggc ggcaaattga
      421 aaaattaatt gccactttac atgccagtct gcaacaacgc tggagcgtag ccgatatggc
      481 tgccacgatc ccctgtagcg aagcctggtt gcgtcgtctg tttttacgct ataccggaaa
      541 gacgccgaaa gaatattacc tcgatgcgcg tctggatctg gcgctatcgc tattaaagca
      601 acaaggaaac tcggttggcg aagtcgctga tacgctcaac ttcttcgact cctttcattt
      661 cagcaaagcc tttaaacata aatttggtta tgcgccttca gccgtgctaa agaatacgga
      721 ccagcaccca acggatgcca gtccacacaa ttaacgtaac tctggccagt acgctttgtt
      781 ggcctccagc aactcatcca gaatagcttt ggcgaccgag gcgctcggta ccgttttcga
      841 caacgtaatc gcctgccaca ggtgctgata tgaacgctgt tcccaggcgt ccacgaccag
      901 tttttccacc gccacttgct gactcattaa ccctttctga aactgcggga tatcgccgac
      961 caccagtggt tctggtccgt tgtgcccgac cagacacggg atctcgacca tagcttcatc
     1021 atcaaagtta tggattgccc cgttgttagg cacaatcagc aacatccgct cctgagtgtt
     1081 gaaggcaatt gccgccgcca gatcgacgat gtatgacgca tgttcgtcaa tttccagctt
     1141 gcccgccgct gaatttcctg ccgccgtaat ggcgcggcaa gcatcgaaaa cctgtttctc
     1201 gcgatgttcc attacctcat tcgcgcgagt atgttcaggg ttggaatgct gaacgacata
     1261 atccgggaag agataatatt tcagataggt gttcggcaat gtatccgggt ccagtgcctg
     1321 gacatcccgc gcttttgcat aggtgtcgtt ccagctcgct tcagtatgtg tatcttgctg
     1381 cggtggaaca taaccgtgct tagagacata ttgccgcagc tggtgcatca ggtcgttgcc
     1441 ctgcaaatct tcaattgatg tccaccagcc aaagtggttc aggccgtagt agcgcacgcg
     1501 catctgtttg cgatcttgca gcccaacaat ttgcgccatc cggctttcaa taccgattgg
     1561 catgtcacag atgttgagga ttttcgcatt cgggcgcaga cgacgcgtgg cttctgcaac
     1621 aattgctgcc gggttggagt agttgagcat ccaggcgttt ggtgaatatt tttccatata
     1681 atccaccagt tccaggacgc cgccaatgga acgcatgccg tacgctattc cgccgggtcc
     1741 gcaagtttcc tgaccaacaa cgccgtggcg cagcgggatt ttttcatcca gttcgcgcat
     1801 cgggtatttg ccgacgcgga tgtgcgccat gacaaaatca acgtcgctga acgccacttc
     1861 aggatcggtg gtgtaactaa acgcaatatc cggcgctttt tctttaagga tgactttaca
     1921 ggcttcggca atcacttcct gccgcgaacc atcgttatca taaaatttca gtgcacgaag
     1981 cgggaaacgg tcctgattcg ccaggagcat caacacgatg cctggcgtaa aggtgcttcc
     2041 accgcctgcg acaaccactg agaatttggt cattatactg cctccgtaat ggcaacattt
     2101 tctgctgatt gatgagaatt aattaagcta tcgagctgtt cacgcagctg ggatacatgc
     2161 agaccaatga tcacctgaat ggcatcgcca ctacggaaga cgccgtgcgc tcccagcttt
     2221 ttaaagactt cgtcatccag cgtttgtgac atgtcatgca gtgcaatgcg tagacgcgtc
     2281 gcacaattgt taatgctgga gatattgccg accccgccca gggcttgcag gataccggca
     2341 gcctgatcca gctctttttt tggctctgcc gcggtggttt ggcctcgcga ggctttgtat
     2401 tcggcttttg agtagagttt cacttccgca tcttcacgtc ccggcgtgca catgttgaat
     2461 tgcagaatca gggtgcggaa aaccacgaag tacagcaggg taaagcacaa cccaatggcg
     2521 atttgggtca gcatcatatc cgcgtggttg ctgaacatcg ggatccagtt ttgcggtaaa
     2581 acctggtcaa tcagacctcc gcccatgttg cccaccacac caaagagata catcacggtc
     2641 gacattgagg ccgcaagcac agcgtgtacc gcaaacagca acggtgaaat gaacaggaag
     2701 gtaaattcca gcggttcggt aattcccacc agcatggcgg ttaaggttgc gggaatcagc
     2761 aaacccgcca cttttacccg attttccggt gcggcagtga agtacatcgc taaagaaatg
     2821 cccacggcac caaagatttt tgagttaccg tgcagggcaa aacctccttc cgggaacaac
     2881 gatttcagcg gctcggcgct caaactgaac tcttgcagat gctgcgccca gtacatctga
     2941 attccgcctt caacagctgc cggaccaaag ataaacggtc cgtagatgaa gtggtgtaaa
     3001 ccggttggga tcagaatacg ttcgaggaag gtgtaaaccc acaccccaag cgcacccgcc
     3061 gaacgcagga acgcttgcag agattcaatc cccatttgta cttttggcca gccgagcaat
     3121 gtcagccagg cacaggggat catcaccagg aaggcgataa tcaccacata agacgtcccc
     3181 tggaaaatgc cgagaaaaac cggcagtttt ttatcgaaca gacggttatg cagcgccgtc
     3241 acaatgccgg aaatgataat tgcgccgata atgctggtat cgagggtttt aatcccggcc
     3301 atcattgtca gaccgctacc tgccaccgcg tcctgagtga aatcgacgcc gaagtagctt
     3361 ccccaggtca ttcccatcgc attgatgaaa tagttccagg tcaggaaact caccatcacc
     3421 gccagacaag cacgcccctg cgcttgctta gcaaggccaa tgggtaaacc gacagcaaaa
     3481 atcagcggca tattacggaa taccgtccaa ccgccctctt caataatgtg tacgatttgc
     3541 gcgaataaac tgttcggatc ggtcagtgat tccccgacaa acatcgggtt ttgcagcaag
     3601 atggcaagac ccaccacaat cccggcgaag ggaaacagca gcactggcgt gaacatcgcg
     3661 ccgccaaagc gttgaatttg actgagcatt tccacatcct cttttctcaa ttctgtaagt
     3721 aggaagcctg attggttatg cggcaggcct ggagcgagga taggaagatg cgatcggcag
     3781 aacatgacga cccaatggga ttcgtgatcg ctttcatgct tttcgttggg cggtagagat
     3841 ctactttttc gcgaaaaagt agatctctgt gagcactggt acacagcatc gaacacatct
     3901 ttaaaaaaaa gatgtttttt caattgatta agcagaactt gtgggcgcat tacccgggct
     3961 tgcaggcaaa aaagagatct agagatgatc tacaaaagca ttgcggagcg gttaagaatt
     4021 cgacttaatt ccgcggattt cacgctaaac agccttcttc ccggtgaaaa aaagctggcg
     4081 gaagagtttg cggtatcgcg gatgaccatc cgtaaagcga ttgacctgct ggtagcgtgg
     4141 gggctggtgg tccgccgcca cggcagcggc acttacctgg tgcgcaaaga tgtgctgcat
     4201 caaaccgcca gcctgaccgg gctggtggag gtgttaaaac ggcagggaaa aacagtcacc
     4261 agccaggtgc tgatttttga aatcatgcct gcgcctccgg caattgccag ccagttgcgg
     4321 attcaaatca acgagcagat ctacttctcc cgtcgcgttc gttttgtgga agggaaaccg
     4381 ctgatgctgg aagacagcta tatgccggta aaactgttcc gtaacctttc gctgcaacat
     4441 ctggaagggt cgaagtttga atatattgaa caagagtgcg ggattttgat tggcggtaat
     4501 tatgaaagcc tgacgccggt gctcgccgat agactgctgg cgcggcaaat gaaggtagcg
     4561 gaacacacgc cactgctgcg gatcacctcg ttgtcatata gcgagagcgg ggagtttttg
     4621 aattattcag tgatgttcag aaatgccagc gaataccagg tggagtacca tttacggcga
     4681 ctccacccgg acaagagtta accgatactc cagaagagca ccgccagtaa ttggggggtg
     4741 ataatgcgca ggaacatcac taacggatag acagtggcgt aagagagcgc cgccgcaccg
     4801 ctggttggat gaagattatt agcaaacgcc agcgccggag gatcggtcat ggaacctgcc
     4861 agcatcccgc acatggtcag gtaattcatt ttggctaaca tccgcgccag aatgccaaca
     4921 gtaatcagcg gaacggcggt gatcagggca ccataaccaa tccagcttag cccttcgcca
     4981 ttgaccaggg tattcacaaa atccccacca gatttcagac caacgaccga gaggaacagc
     5041 acgatcccca gttcccgcag cgcgaggttg gcacttggcg gcataaacca gtacagcttg
     5101 ccgatactgc cgatacgccc gaggatcaac gccataatca gcggaccgcc cgccagcccc
     5161 agtttcaacg cggccgggaa tcctggcaca aagacgggaa tagaacctaa caatacgcca
     5221 agcccgatgc caataaacac tggcagcatc tgaacctgtt gcagtttttg ctgcgcattc
     5281 cccagcacat tggcaacggc atcaattgcg gacggacgcc ccaccaggtt gaggatatcg
     5341 ccgaactgca ggctgatatc gccactggcg accagttcga ccccggcacg gttcaggcgc
     5401 gagataacaa cgtcatagcg ttctttaaaa tgcaggtcgc gaatacgctt accaagcacg
     5461 ttttcattgg tgaccaccac acgctcgacg cgcaaatcag tgcctttcgt ggacagcgaa
     5521 gtatcgacct cctgaccaat caccagttgc gcattatgta aatccgctgg ctgacccacc
     5581 agatgcagca aatcgcccag ttggataatg gtatctggcg aaggaacttt tagggtttct
     5641 tcgcgtttca gacgcgagca gataattttg tcgccgttga gaatcggtac atctttaatg
     5701 gctaaatcat gcaggttagg gttctcaacg cgaatattga tagtcttgat cagcgcgccg
     5761 ccattggtgc gtgaagactc gtgctgctgg gcttctgtct cgacattgac gcggaaaata
     5821 acccgcaaca tccacatggt gaacaatatc ccgcaaatgc cgaatggata cgccatcgcg
     5881 taactcatcc ccatctgatc gaccatttcc attggtgtac ccaggtcgcg caaaatctgc
     5941 tgccctgccc ccagcgctgg cgtattggtt accgcaccgg agaaaatccc cagcactacc
     6001 ggcagtggaa tatcaaacag tttatgcagg atggcggtaa ccagaccacc gatgatgacg
     6061 atcagaacag caaacaggtt gaggcgtaat ccggagacgc gcaatgaggc aaagaagccc
     6121 ggccctacct gaatcccgat ggtataaacg aacaggatca ggccaaattc ctgaataaca
     6181 tgcagcatat cgctacttaa tgtcatcccc gcctgagaaa caaaatggcc gacgatgatc
     6241 ccaccaaaca gcacgccgcc aatacctaat cctatgccgc gaaatttgac gttgccgata
     6301 aacaaaccga cgactgccac caaagccaaa atactgaccg ttaatgctat atcactcatc
     6361 gtccatttct cttgagataa cattttaatt gctacggatt atggctgaat cggtgaagca
     6421 gatttgcctt atagcgcaca ttatcacggg aaagaacgtg ccgaaatatc ttaaacagtc
     6481 gaaatttaac gcattgttga agaagaaaga caaaaaaagc cccaccagaa tggcggggca
     6541 aagagaatag ctagttagct atttaacgcg ggacgttcgc tgatagcgat acgctgcgct
     6601 gcgatgggtt caggctcatt acgaattaaa tcaatatgca gtaaaccgtt tacgaaggtt
     6661 gcgccagaga cttccatatt ttcagccagc gtaaagctca ggctaaatgg ctgattcata
     6721 agcccttgat gcagccattt tttctcttct tttggctgct ccggcgtgcc ttttacgctc
     6781 aggcgcgtac cttccagttg aatctctaaa tcttcctgac ggaaacctgc cagtgcaagg
     6841 gtaatgcggt agtggttatc gtcgcttttc tcaatgttgt acggcgggaa gctctgactt
     6901 tcaccggcgt tttgcagtgc gttggccagt ttgtcaaaac cgatccattg acgcatcagt
     6961 ggggataaat cgaagttacg catagtcatt tctccttcta agaagcgagt aagtacctgc
     7021 aaatccgaag attcgcatat gctccctgac ggcgagcatg gagatgtcag gccgcgcaag
     7081 gcggccttag gaaattagtt gatttcgata cggcgcggtt ttttcgcttc cggaatcacg
     7141 cgttcgagat cgatatacag caaaccattt actaggttag caccacgaac atgaatgttc
     7201 tcagctaact ggaatttgcg ttcaaagttg cgttcagcga tgccctggta cagataggtg
     7261 cgctcttttt gttcgtcggc gtgagcacct ttcaccacca gcagattatc ctgggcggta
     7321 atttccagtt cgctctcagc aaaaccagcc acagcgatag caatgcggta atggttttcg
     7381 tctaccagtt caacgttata cggagggtag ccgccattac tctggctctg gttgttttct
     7441 aagtggttaa acaaacggtc aaatccaata gcagaacggt aaagcgggga taaatcaaag
     7501 ttacgcataa tcaatagctc ctgaaatcag cgagaatgta agaccttcca caatggacag
     7561 gtcaggtagc cagaacaccc atcaggcgcg tcctcatcgg ctacgatgta aaaatgggtc
     7621 tggaaatgaa cttttcaagc cttatcagcg gacttttttt tgcagtttat ggtctattgc
     7681 atagactgag ggggcagcac gaaacgttaa agtgcgatag ccgccacaga gcgacgtaat
     7741 gatgatgtta ttttcagcaa gggatcgcta taactcatca ggccaacacc cacgatcaac
     7801 agatgaattt attatgataa gaaatgtgtt gttagcgttc atgatatgca gcggaatggc
     7861 attactcgga ggatgctcca gcgtgatgtc ccataccgga gggaaagaag gaacgtatcc
     7921 cggcacccgc gccagcgcca ccatgattgg cgatgatgaa accaactggg gcactaagtc
     7981 gctggcgatt ctcgatatgc cgtttaccgc tgtgatggac acgcttttat taccgtggga
     8041 tgtgttccgc aaagacagct cggtgcgctc acgcgttgaa aaaagcgaag ccaacgcgca
     8101 agccaccaat gcggttattc caccggccag aatgcctgat aactgattta gcgatcagtt
     8161 tcggtgatcc acagctggcc gccttccatc cacgccagcc actgaccatc cggcgagaag
     8221 accacggcgt ccgcagaagg tggattggcg tggttttcgg ttaaatactc aacctcgccg
     8281 ctttgcgcat gggcacaagc aattcgatta cccagcacaa agcccaacca ttctcctgac
     8341 ggatgccagt taaatgcaga ctgaatatcc gttttgttat gggttaactg gcgcggctcg
     8401 ccgccctgtg gcgagataag ccacagttgc acaatgccgt tatcatctcg cattaaaaac
     8461 gcgatttgcg taccctgcgg attacagcgc acccagtggc gcgggacgtt gactaacccc
     8521 ggataagccc gatggtgggt aaaggttaaa cgtcgctgca cgatgccacg cggtggcgcg
     8581 ggcagggttg tttccgttcc acttaacggc gcatcacctg ctgctttcca gccagcttca
     8641 tcttgcggta actcaacgat aaacagctcc ggcactttct cgccctttgg cgaaagtgtg
     8701 tcgccaataa acgccagcgc gtgatttcct acccatcctt cttcataagc acgattgatt
     8761 tcatcgctgc caggctgcgg cgtgggcgtg gttttactca ccagcacgca ccagtggcta
     8821 ccgctgtatt cacgcggatg ctgcttttgt acgttgaccg ggccaaacgg tgcagcaacc
     8881 ccgacgtttc gcaaatccag cgccgaatcg aactgatgca ttacatggtc gttataggta
     8941 aaactcacca tttcaccgtt cgggctaaag acatgcacat ggctgccgcc acgcagcgcc
     9001 cctgatgtgt acggaacggt aatatccatt gcatcgagat tgttcatctt gccgccttca
     9061 acaatcacgc cgcgacggtg atggaaatcg taatgccatg tttcatgagg attttcaggt
     9121 ccgtggataa agacatattt ctctgacttt ggatgaacgg tcaccacgcc gacgtgcgcg
     9181 ccctgtgaca cgcgatagat aacctcgacc tcgccggtat ggatattcac acgctcaatg
     9241 gtctcgccgg taaacgacgc gccagaagga cgcacgtcaa ataccagcca ctggctgtcg
     9301 ggcgtccagg tattggtatt ggtgagcagg tgattacggg gagcaaaggt tatctgtttc
     9361 atggcgcggt cctgatacaa aacggttccc gccatcatac ttcacaagga cttcactttc
     9421 agacaatagt cttttgccat cgtgatgaat gtgagtgaaa agatggaaca tttcgacgtg
     9481 gcgattattg gcctcggccc ggcagggtcg gcgttggcac gaaagttagc cggcaaaatg
     9541 caggtgatcg cgctggataa aaagcaccag catggtactg aaggtttcag caagccttgt
     9601 ggcggtctgc tggcaccgga cgcgcagcga tcttttattc gcgatggact gacgcttcct
     9661 gtcgatgtga tcgccaatcc acagattttc agcgtcaaaa ctgtcgacgt cgccgcatcg
     9721 ctcacgcgta actaccagcg aagctatatc aatattaatc gccatgcttt cgacttgtgg
     9781 atgaaatcgc tgatccccgc cagcgttgag gtttatcacg atagcctgtg ccggaaaatc
     9841 tggcgtgagg atgataaatg gcatgtcatt tttcgtgcag acggttggga gcagcatatt
     9901 actgcccgct atctggtcgg tgcagatggt gccaactcga tggtgcggcg atatctctac
     9961 ccggaccatc agattcgtaa atatgtcgct atccagcagt ggttcgcaga gaaacatccg
    10021 gtgccgttct actcatgcat ctttgataat gcgataactg actgttactc atggagtatc
    10081 agcaaagacg gttattttat ctttggcggt gcctatccaa tgaaagacgg tcagacgcgt
    10141 ttcacgacgc tgaaagagaa aatgagcgcc tttcaattcc agtttggtaa ggcggtaaaa
    10201 agcgaaaaat gcacggtgct atttccctca cgctggcagg attttgtctg cggtaaggac
    10261 aacgcctttc tgattggtga agcggcggga tttatcagcg ccagctcgct ggaggggata
    10321 agctatgcgc tggatagcgc agagattctg cgttcggtgt tactgaagca gccagagaag
    10381 atcaacgcag cctactggca cgccacccgc aaactgcgtt taaaactctt cggcaagata
    10441 gtaaaaagcc gatgcctgac cgcaccggct ttaagaaagt ggattatgcg cagtggtatg
    10501 gcgcatattc cacagttgaa agattagcca acgcgcgtca catcgcccac cagcaggatg
    10561 taagagagcg cgccaatcaa cgcgacggcg gagatataaa ccagtgcagg tgcgaaccca
    10621 tagccttgca acagatatta gtccacaaaa aaacacctca taagaaaagg tcttatatcg
    10681 ggcagaaatc tgaaaaggga gatcaggcgc taaacaggag ggaaagaaga gtatgctttc
    10741 aacggcttag ctactcgttt aaaggattaa tcatgaagtt gaattttaag ggatttttta
    10801 aggctgccag tttattccca ctggcgctga tgctttcagg ctgtatctcg tatgctctgg
    10861 tttcccatac cgcaaagggt agttcaggaa agtatcaatc gcagtcagac accatcactg
    10921 ggctatcgca ggcaaaagat agtaatggaa caaaaggcta tgtttttgta ggggaatcgc
    10981 tggattacct tatcactgat ggtgccgatg acatcgttaa gatgctcaat gatccagcac
    11041 ttaaccggca caatattcag gttgccaatg acgcaagatt tgttttaaat gcggggaaaa
    11101 agaaatttac cggcacaata tcgctttact actactggaa taacgaagaa gaaaaggcac
    11161 tggcaacgca ttatggtttt gcctgtggtg ttcaacactg taccaggtca ctggaaaacc
    11221 taaaaggcac aatccatgag aaaaataaaa acatggatta ctcaaaggtg atggcgttct
    11281 accatccatt taaagtgcga ttttatgaat actattcacc cagaggcatt ccggatggtg
    11341 tttccgcagc attactgcca gtgactgtta cgctggacat cattactgcg ccgctgcaat
    11401 ttctggttgt atatgcagta aaccaataat cagtaagcgg gcaaacgcgt ttatgctgtt
    11461 tgcccgccca cagattaatt cagcacatac ttctcaatag caaacgccac gccatcttca
    11521 aggttggatt tggtgacaaa gttcgccact tctttcaccg aaggaatagc attatccatt
    11581 gccacaccga cgcctgcata ttcaatcatt gcgatgtcgt tttcctgatc gccaatcgcc
    11641 atgatttctt ccggtttaat acctaacacg tcggccagtg atttcacacc cgtacctttg
    11701 ttaacgcgtt tatcaaggat ttcgaggaag tacggcgcac ttttcagcac ggtatatttc
    11761 tctttcactt cctgcggaat acgcgcgata gcctggtcga ggatggcggg ttcatcaatc
    11821 atcatcactt tcaggaactg ggtattgggg tccattttct ccgcttcgca gaacaccagc
    11881 ggaatggtgg caacgaagga ttcatgcacc gtgtagtagc tgatatcacg gttggcggtg
    11941 tacagcgtgg tgcggtccag ggcgtggaaa tgagaaccga cttcgcgaga gagtttttcc
    12001 aggaaacgat agtcgtcata gctgagagca gtttgcgcca cggtgttacc atcagcggcc
    12061 ttctgtacca gcgcgccgtt ataagtaatg cagtagtcgc ccggctgttc catatgcagc
    12121 tctttcaggt aattgtgaac acctgcataa gggcgacccg tcgttagcac gacattcacg
    12181 ccacgggcgc gagctgcggc aatcgcattt ttaacggcag gtgaaatggt gtgatcgggc
    12241 agcagaaggg tgccatccat atcgatagca atgagtttga tagccatgag ttccccaggt
    12301 agattggttc ctgacccatg ctaacgcgat tccgctcaaa aatcagtaca acacccgagg
    12361 gaaaaggggg atgcaacgcg ggttgcatgc tccccatttg cttagcggaa gagtttccct
    12421 ttcagcagtt ccatgcctgc ggaaagcaga tcgttattgg cttgtggtga cacttcacct
    12481 tgcggtgaga gcgcatcaat aatcttcggc aattgttctg ccagtaaaca ggaagccgta
    12541 ctggtatcca cgccaagttt ttgtccaaga tcggataccg cgtttgtgcc gagtgctgat
    12601 tccagttgct cgccgctaac tggttgattg ccctgttgat tactcagcca ggttgagaga
    12661 atggccccta agccgccact ttgcagtttt tccagcagca cctgaatgcc accctgctcc
    12721 tcaacccaac ttaaaatcgc ctgatatttc cccgcatcgc ctttcagaaa ggcgccgaca
    12781 acttcatcaa aaagccccat gataatcacc tgtaaagcgt tacgtgttga cccaaaaagt
    12841 atagaactgc gggatgataa ttgcggattg cagaaataaa aagggcggag atgatctccg
    12901 cccttttctt atagcttctt gccggatgcg gcgtgaacgc cttatccggc ctacaaaaac
    12961 atgcaaattc aatatattgc aggagtctcg taggcctgat aagcgtagcg catcaggcac
    13021 gctcgcattg ttaacgccat taaatatcaa tattcgccgc tttcagggcg ttctcttcaa
    13081 taaacgcacg gcgcggttca acggcgtcgc ccatcagcgt ggtgaacaac tggtcagcgg
    13141 caatcgcatc cttaacggta acgcgcagca tacgacggct ttccgggtcc atggtggttt
    13201 cccacagctg ttccgggttc atctcgccca gacctttata acgctggatg gagaggccgc
    13261 gacgggactc tttcaccagc cagtccagcg cctgctcgaa gctggctacc ggctgacgac
    13321 gctcgccacg ttcgataaac gcatcttctt ccagcaagcc acgcagtttc tcacccagcg
    13381 tgcagatacg acgatattcg ccaccagtga taaactcgtg atccagcgga tagtcagtat
    13441 ccacaccgtg ggtacgcacg cgaacaatcg gctcgaacag gttttgttcg gcattggtgt
    13501 ggacatcaaa cttccactgg ctgccgtgct gttctttgtc gttcagttcg ctgaccagcg
    13561 cgttcaccca ccgggtaacg gtctgctcat cagagaggtc ggcttccgtc agcgtcggct
    13621 gatagataag ctctttcagc attgctttcg gataacgacg ctccatgcgg ttgatcattt
    13681 tctgcgtcgc gttgtactca gacaccagtt tctctaacgc ttcgccagcc aatgccggtg
    13741 cgctggcgtt ggtgtgcagc gttgcgccgt ccagcgcgat agagatctgg tactgatcca
    13801 tcgcttcgtc gtctttaatg tactgttcct gcttgccttt cttcactttg tacagcggcg
    13861 gctgagcgat gtagacgtga ccgcgttcaa caatttccgg catctgacga tagaagaagg
    13921 tcaacagcag cgtacgaatg tgcgagccgt cgacgtccgc atcggtcatg atgatgatgc
    13981 tgtgataacg cagtttgtcc gggttgtact cgtcacgacc gataccacaa ccaagcgcgg
    14041 tgataagcgt cgccacttcc tgagaagaga gcatcttatc gaagcgcgct ttctcgacgt
    14101 tgaggatttt acccttcagc ggcagaatcg cctggttctt gcggttacgc ccctgcttcg
    14161 cagagccgcc cgcggagtcc ccttccacaa ggtacagttc ggaaagcgcc ggatcgcgtt
    14221 cctggcagtc tgccagtttg cccggcaggc ccgctaagtc gagcgcacct ttacggcggg
    14281 tcatttcacg cgcgcgacgc gccgcttcac gggcacgggc agcatcgata attttgccaa
    14341 ccacgatttt cgcgtcagtt gggttttcca gcaggtattc cgccaacagt tcgttcatct
    14401 gctgttcaac cgccgatttc acctcagaag aaaccagttt gtctttggtc tgggaggaga
    14461 atttcgggtc cggcactttt acggaaacga ccgcaatcag gccttcacgc gcatcgtcac
    14521 cggtggcgct gactttggct tttttgctgt agccttcttt gtccatgtag gcattcaggg
    14581 tacgggtcat cgccgcacgg aagcctgcca ggtgagtacc gccgtcacgc tgcggaatgt
    14641 tgttggtaaa gcagtagatg ttttcctgga agccatcgtt ccactgcagc gccacttcaa
    14701 cgccgatacc gtctttttcg gtggagaagt agaagatatt tgggtggatc ggcgttttgt
    14761 tcttgttcag atattcaacg aacgctttga tgccgccttc atagtggaag tggtcttctt
    14821 tgccgtcacg cttgtcgcgc agacgaatgg aaacgccgga gttgaggaac gacaactcac
    14881 gcagacgttt cgccagaatt tcatattcga actcggtcac attggtgaag gtttcgaggc
    14941 tgggccagaa acgcaccatg gtgccggttt tttcagtctc gccggtaacc gccagcggtg
    15001 cctgcggtac accgtgttcg tagatctgac ggtgaatttt accctcgcgc tgaataacca
    15061 gctccagttt ttgcgacagg gcgtttacta ccgaaacacc aacgccgtgc agaccgccgg
    15121 acactttata ggagttatcg tcaaatttac cgcctgcgtg cagaacggtc atgatcactt
    15181 ccgccgccga tacgccctct tccgggtgaa taccggtcgg aatgccgcgc ccgtcatcct
    15241 gtacagagac agagttatcg gcgtgaatgg tgacgataat ttctttacag tgacccgcga
    15301 gcgcttcgtc gatagcgtta tctaccacct cgaataccat gtggtgcaga ccggtgccgt
    15361 catccgtgtc gccgatatac atacccgggc gcttacgcac cgcatccagc cctttcagga
    15421 ctttgatact ggaggagtca taagaattcg acatcaacgt ttctcgctca tttatacttg
    15481 ggttaatccg ttattttacc cttttccacg gtaaacatct tcgaattttc gtccgacatg
    15541 tctataacgt gttcagcact gatcgcgctg acaaagacct gcgattgcgt agcttttaag
    15601 cggctggcaa gcagcccgcg acgctcatca tcaagctcag aggcaaaatc atctatcagg
    15661 tagagacacc gccgcccgct ttcacgggtg aggaactctc cttgcgccag acgtaaggcg
    15721 cacatcaaca gcttaagctg cccacgcgat aaggtatctt ccaccggcgc accgtcggcg
    15781 cgaatgcgta agtccgcttt atgcgggcca tgcgcggtgt aggttagctg gcgatcgcgt
    15841 tcaaaattac gttccagcac ctcagcatat tctgtctctt tctcccagcc gcgctggaaa
    15901 gagaaagtca gagaaaactc agggagaaat tgcttacagg tatcagccat atcggccgcg
    15961 ataccggcgc tatactccgc gcgccaggta ctgatttgct ccgccagcgg gatcagttct
    16021 ttatcccacg ggcgtagctg ttcgtaacgt gtcacctggc gcagcgccgc attgcgctgc
    16081 ttgagcaatc gcttgagatt gctccaggcg gtgaaaaatc cgggttcgtt gtgaaagcat
    16141 ccccagtcga ggaatgctct tctgtatttg gggccgccgt tgagtaaagt aaacccttct
    16201 ggcgttatca gctgcattgg catcaggtgc gccagttccg cgactttatg cccgtcagta
    16261 ccgtcgatgc ggactttgct gtcgccctgt ttgtctttgg ttaagccaat cgccgtctcg
    16321 cgctcttcgc cctgtaatcg cccgtggaga acaaacgcct cctgctcgtg gcgaatgacg
    16381 cgaccaatct gcaaactgcg aaacgcccga ccatggccga gcgtatagat ggcttccagc
    16441 acgctggttt tgccactgcc gttggcacct accagaaagt taaagccggg agataaagcg
    16501 agatccgcgg tttcaatgtt gcggaaatcg cggatcaaca agcgggtgag ggacattaca
    16561 gtctcattgg catgacaaca taagccgcgc tctggctggc tgcatcttca atctgcacgc
    16621 tggaaaccga atcggtcagc atcatgcgga cgttttcgca tttcagcgcg ttcagaacat
    16681 ccagcacata gctgacgttg aagccgattt ccatctccgc accgctatag gtaacgtcga
    16741 ggatctcttc cgcttcttcc tgttccgggt tgttggcggt gattttcagc tggttttcgc
    16801 tgacatacag gcgcacaccg cggaatttct cgttagagag aattgccgcg cgagcaaacg
    16861 cctgcttgag gagatcgcag cctgcttcca gatgtttgtc cggattcttc ggcagaacgc
    16921 ggcggtaatc cgggaagcga ccatccacca gtttagaggt gaagataaag tcgccaacgt
    16981 gggcgcgaat attgttgctg ccaatctgca cacgcagcgg attatcgccg ccgtcgagca
    17041 tacgcatcag ttcaatcacg cctttacgcg gcacgatcac cgaatggctt ggcaaagatt
    17101 gaccaattgg cattgaacag accgccagac ggtggccgtc ggtcgctacg gtgcgcagtt
    17161 cttcaccttc ggtttcaaac agcataccgt ttaagtaata gcgaacgtcc tgatgcgcca
    17221 tagaaaactg ggtcgcttca atcagacgct tcatcgtcgc ctgcggcagg gtaaattcga
    17281 cttcactctg ccagtcatcg aggttcggga aatccgccgc tggcagggta gacagcgaaa
    17341 aacggctacg cccggagcgc accagcatcc gctcaccttc cagctgcacg gcaatttccg
    17401 cgccttcagg cagaccacgg cagatatcaa agaatttgcg cgccggaacg gtcgtcgctc
    17461 ctggctcgtg tggctgaacc agcgcaacgc gtgccaccat ttccatctcg agatcagtac
    17521 cggtcagcga caacgtaccg tcagcaacct gtaacagcag attaccgaga atcggtagcg
    17581 taggacgacc acctaacgga ccgctcacct gttgtagcgg ttttaataaa tgctcacgtt
    17641 ctacggtaaa tttcataggt ttacgatgac aatgttctga ttaaatttga aaaatcttct
    17701 ttgatatcgt ggctctcttc acgcagctgc tcgatcttcc ggcaggcatg aagcaccgta
    17761 gtgtggtcac gaccaccaaa cgcatcgcca atctctggca gactgtggtt agtcagctct
    17821 ttcgccagcg ccatcgccat ctgacgcgga cgagccaccg agcgggatcg acgcttggaa
    17881 aggagatccg cgactttgat cttgtagtac tccgccaccg tcttctgaat attgtcgatg
    17941 gtgaccagtt tttcctgcaa tgccagcaag tcgcgcagcg cctcacgcac gaagtcgata
    18001 gtgatcgccc gtccggtaaa gttggcgttg gcaatgacgc ggttcagcgc gccttccagc
    18061 tcacgtacgt tagatcgtag acgcttggcg ataaagaacg ccacttcgcc tggcaaacga
    18121 atgtcatttt cgtcggcctt tttcatcagg atcgccacgc gggtttctaa ctctggcggt
    18181 tcgatcgcca cagtcagccc ccagccgaag cgggatttca aacgatcctc aacgccgttg
    18241 atctctttcg gatagcgatc cgaggtgaga atgatctgtt gattaccttc cagcagggcg
    18301 ttgaaggtgt ggaaaaactc ttcctgagat cgttctttat tagcaaaaaa ctgaatatcg
    18361 tcgatcagca gtgcatctac ggaacggtag tagcgtttaa actcttcgat cgcgttgttt
    18421 tgcagggctt taaccatgtc ctgaacaaag cgctcggagt gcatataaac cactttggca
    18481 ttcggcttgc gcgccataat gccgttaccc accgcatgca gcaggtgagt tttacccaga
    18541 cccgtgccgc cataaaggaa caacgggtta taggcaccgc cagggttatc tgccacctgg
    18601 cgagccgccg cgcgcgccag ttggttagat ttaccttcaa cgaagttatc aaacgtgtgt
    18661 ttgacgttta cgttagaacg ataggtcggt tctgccgggg ccgggacgtt atcccaacct
    18721 gagcgcgtag aaggcgcagc acgttgcggc tgcgtttgcg ccacctgtgc aggggccgcg
    18781 acgttgctcg tcactgccgc ttgtggcgtt tgcgtcaccg atttggtgcc gacttcaaaa
    18841 cgcagctgtg gggcatccgc tccgcagaaa ctggttagca gtccattgat attattaagg
    18901 tacttgtccc gtacccaatc gaggacaaaa cggtttggcg cgtacagggc cagcgtgtta
    18961 tcgctcagtt ccgcctgcaa tgggcgtatc cacatactga attctgtggc tggtaactca
    19021 tcctgcaatc gggcaagaca ctgctgccaa agcgaaagtg acacggcgga ctccactcga
    19081 acaaaagtcg ataatgacta aggctgaaac attcatgatt gttgacgtac gtcgaaaaga
    19141 ccctgcttga gggtgacgca cgaaccgctg tctgcggtta tatgcccgat caagatcctg
    19201 caaaacgatc gggaccgcgg atcatagcct aaactgcgca agagatcttc tgtttctcac
    19261 agattttccc gatttatcca caggatgatc cagaactcgc taagtgtaaa cgatcctgcc
    19321 gcgaggcggg cacgatttac gccgcatatt ggaaaattta atgaccatag acaaaaattg
    19381 gcttaatcga tctaataaag atccaggacg atccttgcgc tttacccatc agcccgtata
    19441 atcctccacc cggcgcgcca tgctggtttt cactggtgtg aggccgtacg ttttccctgc
    19501 gaaaaggtgc ggaaaagcgc ggtaaataag gaaagagaat tgactccgga gtgtacaatt
    19561 attacaatcc ggcctcttta atcacccatg gcttcggtgt ccatcgtttc atttttcggc
    19621 ggatatccaa taaagccatt gaatttattc aagtttaggt agaaatcgcc atgaaacgca
    19681 cttttcaacc gtctgtactg aagcgcaacc gttctcacgg cttccgtgct cgtatggcta
    19741 ctaaaaatgg tcgtcaggtt ctggcacgtc gtcgtgctaa aggccgcgct cgtctgaccg
    19801 tttctaagta ataaagctaa cccctgagtg gttaagctcg catttcccag ggagttacgc
    19861 ttgttaactc ccagtcaatt cacattcgtc ttccagcagc cacaacgggc tggcacgccg
    19921 caaattacca ttctcggccg cctgaattcg ctggggcatc cccgtatcgg tcttacagtc
    19981 gccaagaaaa acgttcgacg cgcccatgaa cgcaatcgga ttaaacgtct gacgcgtgaa
    20041 agcttccgtc tgcgccaaca tgaactcccg gctatggatt tcgtggtggt ggcgaaaaaa
    20101 ggggttgcca acctcgataa ccgtgctctc tcggaagcgt tggaaaaatt atggcgccgc
    20161 cactgtcgcc tggctcgcgg gtcctgatag ccctcattcg ggtctatcaa cgcctgatta
    20221 gtccgctact cgggccgcat tgtcgtttca ctccaacctg ttcaagctac ggaattgagg
    20281 cattgcgcag gtttggagtg ataaaaggca gttggttgac ggtgaaacgc gtattaaaat
    20341 gccacccttt acaccctggt ggtgacgatc ccgtcccgcc cggaccattt aataccagag
    20401 aacactaacg atggattcgc aacgcaatct tttagtcatc gctttgctgt tcgtgtcttt
    20461 catgatctgg caagcctggg agcaggataa aaacccgcaa cctcaggccc aacagaccac
    20521 gcagacaacg accaccgcag cgggtagcgc cgccgaccag ggcgtaccgg ccagtggcca
    20581 ggggaaactg atctcggtta agaccgacgt gcttgatctg accatcaaca cccgtggtgg
    20641 tgatgttgag caagctctgc tgcctgctta cccgaaagag ctgaactcta cccagccgtt
    20701 ccagctgctg gaaacttcac cgcagtttat ttatcaggca cagagcggtc tgaccggtcg
    20761 tgatggccca gataacccgg ctaacggccc gcgtccgctg tataacgttg aaaaagacgc
    20821 ttatgtgctg gctgaaggtc aaaacgaact gcaggtgccg atgacgtata ccgacgcggc
    20881 aggcaacacg tttaccaaaa cgtttgtcct gaaacggggt gattacgctg tcaacgtcaa
    20941 ctacaacgtg cagaacgctg gcgagaaacc gctggaaatc tccacctttg gtcagttgaa
    21001 gcaatccatc actctgccac cgcatctcga taccggaagc agcaacttcg cactgcacac
    21061 cttccgtggc gcggcgtact ccacgcctga cgagaagtac gagaaataca agttcgatac
    21121 cattgccgat aacgaaaacc tgaacatctc ttcgaaaggt ggttgggtgg caatgctgca
    21181 acagtatttc gcgacggcgt ggatcccgca taacgacggt accaacaact tctatactgc
    21241 taatctgggt aacggcatcg ccgctatcgg ctataaatct cagccggtac tggttcagcc
    21301 tggtcagact ggcgcgatga acagcaccct gtgggttggc ccggaaatcc aggacaaaat
    21361 ggcagctgtt gctccgcacc tggatctgac cgttgattac ggttggttgt ggttcatctc
    21421 tcagccgctg ttcaaactgc tgaaatggat ccatagcttt gtgggtaact ggggcttctc
    21481 cattatcatc atcaccttta tcgttcgtgg catcatgtac ccgctgacca aagcgcagta
    21541 cacctccatg gcgaagatgc gtatgctgca gccgaagatt caggcaatgc gtgagcgtct
    21601 gggcgatgac aaacagcgta tcagccagga aatgatggcg ctgtacaaag ctgagaaggt
    21661 taacccgctg ggcggctgct tcccgctgct gatccagatg ccaatcttcc tggcgttgta
    21721 ctacatgctg atgggttccg ttgaactgcg tcaggcaccg tttgcactgt ggatccacga
    21781 cctgtctgca caggacccgt actacatcct gccgatcctg atgggcgtaa cgatgttctt
    21841 cattcagaag atgtcgccga ccactgtgac cgacccgatg cagcagaaga tcatgacctt
    21901 tatgccggtc atcttcaccg tgttcttcct gtggttcccg tcaggtctgg tgctgtacta
    21961 tatcgtcagc aacctggtaa ccattattca gcagcagctg atttaccgtg gtctggaaaa
    22021 acgtggcctg catagccgcg agaagaaaaa atcctgattc ggtgagtttt cgctaaaata
    22081 agggcggtca gttgaccgcc ttttttcttt tcgtagggcg gataagcacc gcgtatccgc
    22141 cacacaaagc aacaggaaca tcatgagcga taatgacact atcgtagccc aggccacgcc
    22201 tccgggacgt ggcggcgttg gcatcctgcg catctccggc ttcaaagccc gtgaagttgc
    22261 cgaaaccgtg ctgggtaaac tgcctaagcc gcgctacgcc gattatcttc cgtttaaaga
    22321 cgccgatggc agcgtgctcg atcaggggat tgcgctatgg ttccctggcc cgaactcgtt
    22381 caccggcgaa gatgtgctgg aactgcaggg tcatggcggt ccggtgattc tcgacctgct
    22441 gttaaaacgc attctgacca ttcccggcct gcggattgct cgccctggtg agttttccga
    22501 acgcgcgttt cttaacgata aactcgactt agcccaggcc gaggcgattg ccgatcttat
    22561 cgatgccagt tcggaacagg cagcccgttc ggcacttaac tcgctgcaag gcgcattctc
    22621 cgcacgggtc aaccatctgg tggaagccct cacccacttg cgcatttacg tcgaagcggc
    22681 aattgatttc cccgatgaag agatcgattt cctctccgac ggaaaaattg aagcccagct
    22741 taatgacgtg attgccgatc tcgatgcagt gcgtgctgaa gcacgtcagg gtagtttgtt
    22801 gcgcgaaggg atgaaggtgg tgattgccgg acgtcctaat gccggtaaat cgagcctgtt
    22861 aaacgcgctg gctggccgtg aagcagcaat cgtaaccgat atcgccggaa ccacgcgtga
    22921 cgtgttgcgt gagcatatcc acattgacgg aatgccgctg catatcatcg acaccgccgg
    22981 gctacgtgaa gccagcgatg aagtggaacg tattggtatc gagcgcgcgt ggcaagaaat
    23041 tgaacaagcc gaccgcgtgc tgtttatggt cgatggcacc acgacagacg ccgttgatcc
    23101 ggcggagatc tggccggaat ttatcgcccg tctgcccgcg aaactgccga tcaccgtggt
    23161 acgcaataaa gccgatatca ctggcgaaac gctgggaatg agtgaagtga acggtcatgc
    23221 gttgattcgt ctctcggcaa agactggcga aggcgtggac gtgctgcgta accatctcaa
    23281 acagagcatg ggctttgaca ccaacatgga aggcggcttc ctggcgcgtc gtcgccacct
    23341 tcaggcgctg gaacaggcag cggagcattt gcagcaaggt aaagcgcaac tgttgggtgc
    23401 ctgggcgggc gaactgctgg cggaagagtt gcgcctggca cagcagaact taagcgaaat
    23461 caccggggaa tttacctctg atgatttgct ggggcggatt ttctccagct tctgtattgg
    23521 taagtaagtc gtctaaattg atataaaggg cactgttccg ttctggggga aactaactat
    23581 cagaacggaa cagcgcttgt ttttttatga gcatcctgca ccaaaactgc ctttactact
    23641 ggctggagcg actttcaaca tcttgcagtc ctgtctttac agagaacgat attgctttag
    23701 ccgattaagc tcctcttgcg gaaagttaaa ttcgtctatt gctttccaga atagttcttt
    23761 aaatacagga ttgccatttg ccttgcttat tacatatttg ccttcattat tcttcgcttc
    23821 cagttgcatc atcacatctt taagataatt ttcattttca ccaaccacaa ttttgaatat
    23881 tttcagccag agacccatat atatcatccg ctttctctac cgcaagagag aatgacttcc
    23941 catcaaagtc acgggtaaac tgctccaaaa tctcatcgaa agtgaacccg tgttgcaacg
    24001 ccaggcgaat cgccgcattc agatatacat caatgacctg cttactcatt gtcttatagc
    24061 tggcaacata caaagagcaa actgcattat ttctgctcag cgacaggagt tctctcacta
    24121 catctttatc attttcataa tggctggcta tcacttcagc tgtctccatc caggcagcaa
    24181 caatcttcgt atgccctttc actatcgcag aatataagcc tgatgccttc gtccatttgt
    24241 ctgatgctcc cgcc
//
LOCUS       DBFIFI010000054        23889 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.054, whole genome shotgun sequence.
ACCESSION   DBFIFI010000054 DBFIFI010000000
VERSION     DBFIFI010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23889
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.054"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..155)
                     /locus_tag="N7B39_002060"
     CDS             complement(<1..155)
                     /locus_tag="N7B39_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2422046.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRAD
                     SRFELIQ"
     gene            complement(333..1244)
                     /gene="gsiD"
                     /locus_tag="N7B39_002061"
     CDS             complement(333..1244)
                     /gene="gsiD"
                     /locus_tag="N7B39_002061"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HGU2422047.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1247..2167)
                     /gene="gsiC"
                     /locus_tag="N7B39_002062"
     CDS             complement(1247..2167)
                     /gene="gsiC"
                     /locus_tag="N7B39_002062"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HGU2422048.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIARRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2185..3723)
                     /gene="gsiB"
                     /locus_tag="N7B39_002063"
     CDS             complement(2185..3723)
                     /gene="gsiB"
                     /locus_tag="N7B39_002063"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HGU2422049.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQAALLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(3743..5614)
                     /gene="gsiA"
                     /locus_tag="N7B39_002064"
     CDS             complement(3743..5614)
                     /gene="gsiA"
                     /locus_tag="N7B39_002064"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HGU2422050.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKEAVARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(5601..6566)
                     /gene="iaaA"
                     /locus_tag="N7B39_002065"
                     /pseudo
     CDS             complement(5601..6566)
                     /gene="iaaA"
                     /locus_tag="N7B39_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
     gene            6770..8005
                     /gene="moeA"
                     /locus_tag="N7B39_002066"
     CDS             6770..8005
                     /gene="moeA"
                     /locus_tag="N7B39_002066"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HGU2422051.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADITSGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLHRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VEVGEWVEVEPFNALFGGL"
     gene            8005..8754
                     /gene="moeB"
                     /locus_tag="N7B39_002067"
     CDS             8005..8754
                     /gene="moeB"
                     /locus_tag="N7B39_002067"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HGU2422052.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDSVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHNLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFSENVLTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(8933..9595)
                     /gene="fsa"
                     /locus_tag="N7B39_002068"
     CDS             complement(8933..9595)
                     /gene="fsa"
                     /locus_tag="N7B39_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HGU2422053.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLEVVLP
                     ELHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTTEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPRA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWQGAFGRT
                     SI"
     gene            9726..10625
                     /gene="ybiY"
                     /locus_tag="N7B39_002069"
     CDS             9726..10625
                     /gene="ybiY"
                     /locus_tag="N7B39_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HGU2422054.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDPRLCLEGCELCAKTAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQSLTVCGE
                     VKSVEEIMVTVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            10631..13062
                     /gene="ybiW"
                     /locus_tag="N7B39_002070"
                     /pseudo
     CDS             10631..13062
                     /gene="ybiW"
                     /locus_tag="N7B39_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
     gene            13208..14023
                     /gene="ybiV"
                     /locus_tag="N7B39_002071"
     CDS             13208..14023
                     /gene="ybiV"
                     /locus_tag="N7B39_002071"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HGU2422055.1"
                     /translation="MSVKVIVTDMDGTFLNDVKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14175..15440
                     /gene="ybiU"
                     /locus_tag="N7B39_002072"
     CDS             14175..15440
                     /gene="ybiU"
                     /locus_tag="N7B39_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HGU2422056.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPSQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(15608..17200)
                     /gene="ybiT"
                     /locus_tag="N7B39_002073"
     CDS             complement(15608..17200)
                     /gene="ybiT"
                     /locus_tag="N7B39_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HGU2422057.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            17419..18339
                     /gene="ldtB"
                     /locus_tag="N7B39_002074"
     CDS             17419..18339
                     /gene="ldtB"
                     /locus_tag="N7B39_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HGU2422058.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(18397..19515)
                     /gene="ybiR"
                     /locus_tag="N7B39_002075"
     CDS             complement(18397..19515)
                     /gene="ybiR"
                     /locus_tag="N7B39_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HGU2422059.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVTL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(19512..19979)
                     /gene="mntR"
                     /locus_tag="N7B39_002076"
     CDS             complement(19512..19979)
                     /gene="mntR"
                     /locus_tag="N7B39_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HGU2422060.1"
                     /translation="MSRRAGTPTAKKVTQLVNEEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            20165..20293
                     /gene="mntS"
                     /locus_tag="N7B39_002077"
     CDS             20165..20293
                     /gene="mntS"
                     /locus_tag="N7B39_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HGU2422061.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            20565..22148
                     /gene="opgE"
                     /locus_tag="N7B39_002078"
     CDS             20565..22148
                     /gene="opgE"
                     /locus_tag="N7B39_002078"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HGU2422062.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTKGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(22197..22712)
                     /gene="ompX"
                     /locus_tag="N7B39_002079"
     CDS             complement(22197..22712)
                     /gene="ompX"
                     /locus_tag="N7B39_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HGU2422063.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(22765..22830)
                     /locus_tag="N7B39_002080"
     CDS             complement(22765..22830)
                     /locus_tag="N7B39_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2422064.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            23065..>23889
                     /gene="rhtA"
                     /locus_tag="N7B39_002081"
     CDS             23065..>23889
                     /gene="rhtA"
                     /locus_tag="N7B39_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HGU2422065.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAA"
BASE COUNT         5816 a         6545 c         5948 g         5580 t
ORIGIN      
        1 ttctgaataa gttcaaagcg gctatcggca cgttttaccg cgcgttccga taccagttga
       61 tagttaatga tgaaaaaaag gccaataaat gacagtgctg ccaataaaaa taaaattatc
      121 agccttatct ttatcttttc gtataaactc agcatgttaa tacatttaac aaaataactg
      181 tctgaacgct gtgccgctgc aaaaacagtc ccttgctgcg gctatttaac caaattaccc
      241 gattctacaa aatcagcaga aagttacaac acttgcttag ggcaataatt tcctgtgagg
      301 tgattactct ttcaagcaat attcaaacgt aactatcctt taattttcgg atccagcgca
      361 tcgcgtaaac catcgcccaa caaattgaac gccagtacag tcagaaaaat agccagggcc
      421 ggaaaaacag cgacatgcgg cgcgataacc atatccgctc gagcctcatt gagcattgct
      481 ccccactctg gtgtcggcgg ctgcgcaccg aggccgagaa atgacaggct ggcggcagag
      541 ataatcgagg taccaatgcg catggtgaaa aacaccacga tagaagagac ggtcccaggc
      601 aggatatgac gcaacaaaat ggtcatatcg ctggcaccaa tactgcgtgc tgactcaata
      661 aaggtttgct gtttcaacac cagcgtgttg ccgcgcacca ggcgggcaaa cgcagggatg
      721 gaaaaaatgg cgactgcaat aatcacgtta gcaatgccgc ttcccaacac cgcaacaaca
      781 gcgatcgcca gtaaaatacc cgggaaggca aacagcacat cgcaaatgcg catgatcagc
      841 cgatcccacc agccttcata atatccagcc aacaagccca gcaacgtccc tatcgccgca
      901 ccgataaaca cggcaaacac gcccgccgcc agcgagattt gcgcaccaac caggacacgg
      961 ctgaaaatgt cgcgccccag tgaatcgacg ccaaaccagt gctgcaaaga aggtccgtta
     1021 ttcagattgt cataatcaaa ataattttcg gcgtcatagg gagcgatcca gcgtgcaaaa
     1081 atggccacca caatcaataa aataacgaat aatgcggcgg tcatcgccat atgctgacgg
     1141 cgaaatcgtc gccagaattc atgccacggt gtacgtacct ggtcaggttt gaccagtggc
     1201 atggcgttta acaccgcctg acgtcgccag ttaaatagtc gcatccttac ttgtacctga
     1261 tagccgggtt aatggcggcg taaagcacat ccaccactaa gttgataaga ataaattcca
     1321 gcgagaaaag cagaatttcc gcctgaatca ccgggtaatc gcgcatttct acggagtcaa
     1381 cgagtaagcg tccaagtccg ggccagttga aaactttctc caccacgatg gaaccaccga
     1441 gcaaaaagcc aaactgtaag cccatcatgg tcactaccgg gatcatcgcg ttgcgtagcc
     1501 cgtgtttgag gacaacccag gtttcgctca cccctttcgc cctcgcggta cgcatataat
     1561 cttcgcttaa aacgtcgaca aacgacgcgc gggtaaagcg cgccatcacg gcggccactg
     1621 ccgcgccgag cgtcagggag ggtaaaatgt agtgctgcca actgtctgct cccacggtag
     1681 gcagccagcc cagttcaacg gagaatacct gaattaaaag catccccagc gcaaatgccg
     1741 gaaacgagat ccccgacacc gcaatggtca tactcaatcg atccggccaa cggttacgcc
     1801 agacggcggc gataattccc gccgccatac caaatatcac cgcccagacc atactggtta
     1861 tggtcagcca cagcgttggc ataaagcgcc tggcaatctc atcggcaacc ggacgacgtg
     1921 acaccatcga caggccaaaa tccccctgca cagcattgct gatatagtgc cagaactggt
     1981 gatacagcgg ctgatccaac cccagctgct gacgcaccag ttctataacc tgcgcatcag
     2041 cttcgggccc ggcaatcaat cgcgccggat cgccgggcag catatgcaca aataaaaaca
     2101 ccagcaccga gacgataaac agcgtcggaa tcaaccccag taatcgtttg ataacgtaat
     2161 taagcattcc actccctgcg ttgcttattg caaatccgcg tcttcaaagc tgaagccggt
     2221 atctggcatg atccaaaaac cggtcaggtt tttactgtgt gccgacacca gtttttctac
     2281 caccagcggg atccacggcg actcttgcca gatgatatcc tgcgccgcct tatataagcg
     2341 ggtcttttcc gccggatcat tagttttcag tgcctgagcc aggaagtcat ccacctgttt
     2401 attgctgtaa aacgcggtat taaacagcgt cggtggccag ttttgcgagg caaacaacgg
     2461 cgatagtgcc cagtccgctt cgccggttga agccgaccag ccagtgtaga acatccgcac
     2521 gccgctctct ttttgccctt taccttcaac ttctgccgcc cgctgtccgg catccatcgc
     2581 agtcacctgg gctttaatcc cgacctgcgc taactgctgc tgggtaaatt gcagcacttt
     2641 ctgcgcggtg ctgtggttat gtgacgacca cagcgtggta ctgaaaccgt tgggatatcc
     2701 cgcctctttc agtaattcgc gcgctttcac tggatcgtaa ggccacggtt tataactttg
     2761 cgcgtaggcg atactcggcg gtaccacacc agtagctggc gttgcatagc ccgcgaaggc
     2821 cactttcacc agcgccggac ggttaatggc gtaattcagc gcctcacgga ccttcgggtt
     2881 atcgaatggc ttttgcgtca cgttcatact gatataacgc tgcataattg acggactggc
     2941 catcaactcg atatttttgt ttttctccag cagtgcggcc tgctcgtaag gaatggggaa
     3001 agcaaactgc gcttcaccgg tttgcagcat tgccgcgcgg gtgttgttat ccgccaccgg
     3061 acgccaggtt atgctgtcca gtttgggcaa tcctggctgc cagtaacccg cgaatttttt
     3121 caccttcaca aaatcggtct gattccaggt atccagttca tacggtccgg ttcccaccgg
     3181 atgaaaacca atctccttgc catatttttc cagcgctgcc ggtgaaatca tcgcggtcgc
     3241 cggatgggca agaatattaa taaacgctga gaacggctgt ttgagggtaa tctttaccgt
     3301 tgtcggatcg atcgcttccg ttttagcaat attcttatac aggttatagc gtttaagatg
     3361 attcgccgga tcgctggccc ggtccagatt cgcttttacc gccgctgcgt tgaaatcggt
     3421 gccatcctgg aatttaattc cttcccgcaa tttcacggtg taagtaatgc cgtcatcgga
     3481 aacggtataa ctctccgcca gcacgttttt cagtttcatc tctttatcca gaccgaacag
     3541 cccctggtaa aacgatttcg ctacggcctg agataacgtg tcattcgcgt catacggatc
     3601 gagcgtggtg aaattcgatc ctaccgccac caccacatct ttggcagcga atgcacaaga
     3661 tgccatcaac gctgtcgcaa tgcccagcgc cactaaccca ctacggtgta cagctcttgc
     3721 cattttattc tccgcctgaa tgttatctac gcatgaatgc gtattctgat tgtggttgtg
     3781 cgacgtaatg ccccggcccg acgcattgca acgagacggc tgccacctct tcgccacgca
     3841 gatgaatatt gctgggaaga tcgtccgaca gcagtacacg ctgcggtcgt tgtcgggacg
     3901 gttcagcgac cggaactgcc gccagtaatt tacgcgtata aggatgctgc gggttttcga
     3961 agaccgcgcg ccgtggacca atttcaacaa tttgcccgag atacatcacc gccacacgat
     4021 gactaatccg ctctaccaca gccatatcgt gggagataaa cagatacgca atgccgaaat
     4081 cacgctggag atcgagcaac aagttgataa tctgcccgcg aattgaaaca tccagcgccg
     4141 aaacggcttc gtcggcaatg atcacttttg gattcaatgc caacgcgcga gcaatgcaga
     4201 tgcgctggcg ctgaccgccg gaaaactcat gcgggtaacg ccaggcatgt tcaggtaaca
     4261 ggcccacgcg ctccagcaac cacgcaacgc gtgcaaccgc ttctttacct ggcaataaac
     4321 cgtgtacgcg cagcggttcg ataatcgaat caccgatggt ctgacgtggg tccagcgaag
     4381 cgtaagggtc ctgaaaaata aactgaatat cgcggcgcaa tgcctgaagt ttgccgggtg
     4441 acaaggtatc gattcgctga ccgttaaaga taatttcgcc gccctgcgat tcgaccaggc
     4501 gcagcaacgc ccgcccggta gtggatttac cgctgccaga ctcgcccacc agcgatagcg
     4561 tttcgccagg ccagagatca aaactgactt tctcaacggc atgcacttcc cgcgttacgc
     4621 gattcaacaa accgctgcgc aaagggaaac gggtgaccag attacgcacc cgtaaaacag
     4681 gttcgccatc caccaccgtt ttctgctcga tggggggggc ctgtttcgct ggatgttcaa
     4741 gcgatatcaa tgggaaacgt cggggataat ctaacccttt catcgcacca agttgcggaa
     4801 cagcagctaa cagcgcacgg gtgtagggat gttgcggtgc atgaaaaatc tgttcgacgg
     4861 tacccgtttc caccgcctcg ccctgataca tcaccagtac ccgatcggca atctctgcca
     4921 ccacgcccat atcgtgagtg ataaagataa cgcccatcga catctctttt tgcaatactt
     4981 tgattaattg caggatctgc gcctgaatag tgacatccag cgcggtggtt ggctcatcgg
     5041 ctatcagcac tgccgggcgg catgacagcg ccatcgcaat catcactcgc tggcgcatcc
     5101 cgccagagag ttgatgcgga taacgtgaaa gaatggtttg tgcctccgga atgcgtacct
     5161 gatccagcat ccgcttcgcc tcgaccatcg cttcttcacg actggcgttc tgatgcagac
     5221 gaattgattc ggcaatctgt tcacccacag taaatactgg gttcagcgat gtcatcggct
     5281 cctgaaatat catcgccata tccgcaccgc gcacatggcg catttgtgca gcgctctgct
     5341 cgctaagttc aatcacatca cgactgcgcc gccgcaacag cattttatcg cactgcacta
     5401 aaccgcccgc ctgttccaac agacgcatca atgccagcgc agtcactgac ttaccggagc
     5461 cggattcgcc aacaattgcc agcgtctcac cgcgttgcag actaaaagag agattgcgga
     5521 ccgcagctat tttctgctgg tcctgcataa aggcaatatt aagattttca accgccagca
     5581 cattaccggc atcaagttca tcactgtgtg gcaacggtgt cccctttttc acggtagata
     5641 ccggtggttg gcgtatcgcc tgcgtagccc caggcgcgat acattccttc ggtgttaaac
     5701 ggtagcgcga cattcccttc atggtcgata gcgattaagc caccgctacc gccaagcgca
     5761 gggagttttt ccattactac ccgctcgcag gcttccgcga gacttaatcc gccgtaatcc
     5821 attaacgcgg cgatgtcata tgccgccagc gcgcggatga agacttcgcc cgtgccggta
     5881 caagaaaccg ccacactggc gttattggcg tagcatccgg cacccactaa ggggctatcg
     5941 ccaactcgtc cgggtaattt attggtcatt ccgcccgtgg acgtggctgc cgccagattg
     6001 ccgtctaaat ccaacgccac ggcccccacg gtgcccattt tctgtttttc atccagtggc
     6061 gcaccgctat ggtcgaggac tgttgcccct tcctcgcgcg ctgccattag ttgttcataa
     6121 cgcaaaggcg tggagaaaat ctccggtgag acgcgctcca tgccatgagc aaacgcaaaa
     6181 ttttctgccc cttcgccaat catcatcaca tgcgggcttt actccatcac cagccgggcg
     6241 gcaagaaccg gattacgcag atgactaacg cccgccaccg caccggcttt cagggtgtta
     6301 ccatccatca cacaggcgtc cagttcatgc gtttcatcac gcgtaaagac ggcgccaatt
     6361 ccggcgttaa acagtggaca ctcttccagc agacgcaccg cttccgtcac cacatccagc
     6421 gcactttcgc ccgcttccag cattttctgc ccggtttcaa caatggcaga caacgcctcg
     6481 atgtagcgta attcctgttg cagactcatc tgcgcgcggc taattgcacc tgcgccacca
     6541 tgaattgcaa tgactgcttt gcccatagta ttcgtccatt aagggtggct cgccactttt
     6601 gctataaata tcagaatttt tatgaattac tcattcaatt tttgaatata gaaagatgga
     6661 tatggcatgt aaaggcagga cccagcgcta cgcccgaggt ggcatacagt ctgtggcgtt
     6721 tttctgacat aataggcaaa ttcgattttg cctccgcagg agtgttttca tggaatttac
     6781 caccggattg atgtcgctcg acaccgcgct taatgagatg ctttctcgcg tcaccccact
     6841 gaccgcccag gaaacgctgc cactggttca gtgttttggt cgtattctgg cgagcgatgt
     6901 cgtttcgccg ctggatgtcc cagggtttga taactccgca atggatggtt acgcggtacg
     6961 tttggccgat atcacctccg gacaaccgct gcccgttgcc ggtaaatcct ttgccggtca
     7021 gccgtaccat ggtgaatggc ctgcgggtac ctgcattcgt attatgaccg gtgcgccggt
     7081 gccggaaggc tgcgaagcgg tggtgatgca ggagcagact gaacaaacgg acaatggcgt
     7141 gcgttttact gctgaagtgc gtagcgggca aaatattcgc cgtcgcggtg aagatatctc
     7201 tgcaggtgcg gttgttttcc cggcggggac tcgcctgact accgcagagc tgccagtaat
     7261 tgcttcactg gggattgccg aagttccggt gattcgtaaa gtgcgtgtag cgcttttttc
     7321 taccggtgat gaactccagt tgccaggcca gccgctgggc gacggccaaa tctacgatac
     7381 caaccgtctc gccgtacacc tgatgttaga acagctgggt tgcgaggtaa tcaacttagg
     7441 gattatccgc gacgatccac atgccctgcg cgccgcattt attgaagccg acagccaggc
     7501 ggatgtggtg atcagttccg gcggtgtttc agtgggtgaa gcggattaca ccaaaacgat
     7561 tcttgaagag ctgggggaga tcgccttctg gaagctggcg attaaaccag gtaaaccgtt
     7621 cgcgttcggt aaactcagca atagctggtt ctgcggcctg ccgggcaacc cggtttcagc
     7681 gacgctgacc ttctatcaac tggtacagcc tttgctggca aaactaagcg gcaacaccgc
     7741 cagcggcctg cccgcgcgcc agcgcgtacg cacagcgtcc cgcctgaaaa aaacgccagg
     7801 acgtcttgat ttccagcgcg gcgtgctgca ccgcaacgcc gatggcgaac tggaagtgac
     7861 gaccaccgga catcagggtt cacatatatt cagctccttt agcctcggca actgctttat
     7921 cgtgctggaa cgcgatcgcg gcaatgtgga agtgggcgaa tgggtggaag tagaaccgtt
     7981 taacgcgttg ttcggaggcc tgtaatggcg gaactcagcg atcaagagat gctgcgctac
     8041 aaccggcaaa tcattctgcg cggttttgat tttgacggtc aggaggcgct gaaagattct
     8101 cgtgtgctgg tcgtcggcct cggtggactc ggctgcgcag cttcgcagta tctggcaagc
     8161 gccggtgtcg gcaacctgac gctgctcgac ttcgactcgg tttcgctctc gaatctgcaa
     8221 cgccagacgc tgcacagtga tgctacggtc gggcaaccga aggtggaatc cgcccgtgac
     8281 gctctgacgc ggatcaaccc acatatcgcg attacgccag tcaatgcact gctggatgac
     8341 gcagaacttg cagcattgat tgctgaacac aatcttgtgc tcgactgtac ggataacgtt
     8401 gcggtacgta atcaactgaa cgcaggctgt tttgccgcga aggtgccgct ggtttccggc
     8461 gcggcaattc gtatggaagg gcaaatcacg gtcttcactt atcaggacgg cgaaccgtgc
     8521 tatcgctgcc ttagccgttt gtttagcgag aacgtgttaa cctgcgtaga agcaggcgtg
     8581 atggcaccgt tgatcggcgt gattggttcg ttgcaggcaa tggaagcgat caaactgctg
     8641 gcaggttatg gcaaaccggc cagcgggaaa atcgtcatgt acgatgcgat gacctgtcag
     8701 tttcgcgaaa tgaaactgat gcgcaatccg gggtgtgagg tgtgcgggca gtaattgcca
     8761 cctccaggcc tgataagacg cgacaagcgt cgcatcaggc atttgctcca gacgccagat
     8821 gcggcgtgaa cgccttatct ggcctacggt tcgggcacag atttccaagc gcggcaggca
     8881 tcggtgcact ccgccacgtc atcagcaatt tatgaggatg cagaacgtcc ggttaaatcg
     8941 acgttctgcc aaacgctccc tgccagtcct gctcaaactt cgccacagcg gcatcaaccg
     9001 ccggatagct aatcatctgt tgtgccacat ccagtggcag agtaattgat tcacatcctg
     9061 ccagtaagca gtccagcgcc tgacgcgggg ttttgaaact cgcggctaac actttcgccc
     9121 gcggcgcatg cattttcaat aactggtgta agtcggtcac agtctgaata ccgctaccgc
     9181 cctgagcatc aatacgatta acgtaaggcg caacatattc cgcacctgcc agcgccgaca
     9241 gcagcccttg tgctgcgcca tataccgcgg ttcccagcgt cggaatccct tccgctttta
     9301 acatcttaat agctgccagc ccctcggtgg tcaccggaac tttcaccacg atatccgcaa
     9361 taatagaacg caatttacgt gcgtcattca ccatcccttc agcagtggta gccattacct
     9421 gggcaaacag acgcccctga ccgcccatcg cttcatgaag ttccggaagc acaacctcca
     9481 gcggtttttt acccgcggcg ataatgcttg ggttagtggt cacacccgcc agcggaaaaa
     9541 tacgtgacag cgccttcacc gcaacaacgt ctgaagtatc cagatacagt tccataacca
     9601 tcctcaaaat gtactttttg tctgaatgta gactatcacg ggaaaactct ctgaacagtt
     9661 gacgcacatc aatctaactt tcattcgaaa gtattttaat atttgaacga aacagagagg
     9721 caactatgat tttcaatatt cagcgctact cgacccatga tggccccggt atccgcacgg
     9781 tcgtatttct taaaggctgt tcgctgggct gccgctggtg tcagaacccg gaaagccgcg
     9841 cccgcacgca ggatctgctg tatgacccac gtctgtgcct ggaaggctgt gagctgtgcg
     9901 ctaagaccgc gccggaagtg attgagcgcg cgctgaatgg tttgcttatt catcgggaaa
     9961 agttaacccc ggagcatctg acggcgttaa ccgactgctg cccgacacag tcgttaaccg
    10021 tgtgtgggga agtgaaaagc gttgaggaga tcatggtgac cgttctgcgc gataaaccgt
    10081 tttacgatcg cagcggcggc ggcttaacac tttctggcgg tgaaccattc atgcagccgg
    10141 aaatggcgac ggcgctactg caagccagcc acgaggccgg cattcatact gcggtagaaa
    10201 cctgcctgca tgtgccgtgg aaatatatcg ccccttctct gccctatatc gatctgtttc
    10261 ttgccgattt aaaacacgtt gccgacgcgc cgtttaaaca gtggaccgac ggtaacgccg
    10321 ccagggtgct ggataacctg aaaaaactcg ccgcagcggg caaaaaaatc attatccgcg
    10381 tgccgctgat tcaaggcttt aatgccgacg aaacctctgt gaaagccatt accgattttg
    10441 ccgccgacga gctgcacgtt ggcgaaattc atttcctgcc ctaccacacg ctgggcatca
    10501 acaaatatca cttacttaat ctgccctatg acgccccgga aaaaccgctt gatgcgccag
    10561 aactgctcga ctttgcccag cagtatgcct gccagaaagg gttaaccgcg accttacgag
    10621 gataacaacc atgaccacac tgaaactgga cacgctcagc gaccgcatta aagcgcacaa
    10681 aaatgcgctg gtgcatattg tgaaaccgcc ggtttgtacc gagcgcgcgc agcactatac
    10741 cgagatgtat caacaacatc tcgataagcc gatccctgta cgtcgcgcgc tggcgctggc
    10801 acatcacctg gcgaatcgca ctatctggat caagcacgat gagttgatca tcggcaacca
    10861 ggcaagcgaa gttcgcgccg cgccgatctt cccggaatat actgtctcgt ggatcgaaaa
    10921 agagattgat gatctggcag atcgtcccgg tgctggcttt gcggtgagcg aagagaacaa
    10981 acgcgttctg catgaagtgt gcccgtggtg gcgcggtcag acggtacagg atcgctgcta
    11041 cggcatgttt accgatgagc aaaaaggtct gctggcgacc ggaatcatta aagcggaagg
    11101 caatatgacc tccggcgatg cgcacctggc ggtgaatttc ccgctgctgc tggaaaaagg
    11161 cttgatggtc tgcgcgagaa agtagcggaa cgtcgctcgc gcatcaatct gacggtactg
    11221 gaagatctgc acggcgaaca gttcctgaaa gcgattgata tcgtgctggt ggcagtcagt
    11281 gaacacattg aacgtttcgc tgccctggcg cgtgcaatgg ccgcgaccga aacccgcgaa
    11341 agccgtcgcg atgaactgct ggcgatggca gaaaactgcg atcttatcgc ccaccagccg
    11401 ccgcagactt tctggcaggc gctgcaactg tgttacttca tccagttgat tttgcagatc
    11461 gaatctaacg gtcactcagt atcgtttggt cgtatggacc agtatctcta cccgtactat
    11521 cgccgcgacg ttgaactcaa ccagacactg gatcgcgaac acgccatcga attgctgcat
    11581 agctgctggc tgaaactgct ggaagtgaac aaaatccgct caggctcaca ctcaaaagcc
    11641 tctgcgggaa gtcctctgta tcagaacgtc accattggcg gacaaaatct ggttgatggt
    11701 caaccaatgg acgcggtgaa tccactctct tacgcgatcc tcgaatcctg cggtcgcctg
    11761 cgttccactc agcctaacct cagcgtgcgt taccacgcgg gaatgagcaa cgatttcctc
    11821 gacgcctgcg tacaggtgat ccgttgcggc ttcgggatgc cggcgttcaa caacgacgaa
    11881 atcgtcatcc cggaatttat taaactcggt attgaaccgc aggatgctta caactatgca
    11941 gcgattggtt gtatcgaaac cgccgtcggt ggcaaatggg gctatcgctg taccggcatg
    12001 agctttatca acttcgcccg cgtgatgctg gcggcgctgg aaggcgggcg tgatgccacc
    12061 agcggcaaag tgttcctgcc acaagaaaaa gcgttgtcgg caggtaactt caacaacttc
    12121 gatgaagtga tggacgcgtg ggatacgcaa atccgttact acacgcgtaa atcaatcgaa
    12181 atcgaatatg tcgtcgacac catgctggaa gagaacgtgc acgatattct ctgctcggcg
    12241 ctggtggatg actgtattga gcgagcgaaa agtatcaagc aaggcggcgc gaaatatgac
    12301 tgggtttctg gcctgcaggt tggcattgcc aacctcggca acagcctggc ggccgtgaag
    12361 aaactggtat ttgaacaggg cgcgattagt cagcaacagc tggctgccgc actggcgaat
    12421 gacttcgacg gcctgactca cgagcagctg cgtcagcggc tgattaacgg tgcgccgaag
    12481 tacggcaacg acgatgatac tgtcgatacg ctgctggctc gcgcttatca gacctatatc
    12541 gacgaactga aacagtacca taacccgcgc tacggtcgtg gtccggttgg cggcaactat
    12601 tacgcgggta cgtcgtctat ctccgctaac gtaccgtttg gcgcgcaaac tatggcaacg
    12661 ccagacggac gtaaagccca ccccccgctg gcagaaggcg caagcccggc ttccggtact
    12721 gatcatcttg gccctactgc ggtcattggc tcggtgggta aactgcctac ggcagcgatt
    12781 ctcggcgggg tgttgctcaa ccagaaactg aatccagcaa cactggagaa cgaatctgac
    12841 aagcagaaac tgatgatcct gctgcgcacc ttctttgaag tgcataaagg ctggcatatt
    12901 cagtacaaca tcgtttcccg cgaaacgctg ctggaggcga aaaaacatcc cgatcagtat
    12961 cgcgatctgg tagtgcgtgt cgcgggttat tccgccttct tcaccgcgct ctctccagac
    13021 gctcaggacg atatcatcgc ccgtaccgaa catatgctgt aatcccctca gcccggcgga
    13081 gacgtcgccg ggctaaatca ttcacatcat caatttcatc cttactttca ttcgaaatat
    13141 aatttgtgct ctgcgtcaca ttgttattac actgttacgg tttcattttc gagccgggag
    13201 cacacctatg agcgtaaaag ttatcgtcac agatatggac ggtacttttc ttaacgacgt
    13261 caaaacgtac aaccaaccac gttttatggc gcaatatcag gaactgaaaa agcgcggcat
    13321 tgagttcgtt gtcgccagcg gtaatcagta ttaccagctt atttcattct ttcctgagct
    13381 aaaggatgag atctcttttg tcgcggaaaa cggcgcactg gtttacgaac atggcaagca
    13441 actgttccac ggcgaactga cccgacatga atcgcggatt gttattggcg agttgctaaa
    13501 agataagcaa ctcaattttg tcgcctgcgg tctgcaaagt gcatatgtca gcgaaaatgc
    13561 ccccgaagca tttgtcgcac tgatggcaaa acactaccat cgcctgaaac ctgtaaaaga
    13621 ttatcaggag attgacgacg tactgttcaa gttttcactc aacctgccgg atgaacaaat
    13681 cccgttagtg atcgacaaac tgcacatagc gctcgatggc attatgaaac ccgtcaccag
    13741 tggttttggc tttatcgacc tgattattcc cggtctacat aaagcaaacg gtatttcgcg
    13801 gttactgaaa cgctgggatc tgtcaccgca aaatgtggta gcgattggcg acagcggtaa
    13861 cgatgcggag atgctgaaaa tggcgcgtta ttcctttgcg atgggcaatg ctgcggaaaa
    13921 cattaaacaa atcgcccgtt acgctaccga tgataataat catgaaggcg cgctgaatgt
    13981 gattcaggct gtactggata acacatcccc ttttaacagc tgacctctct accggagcca
    14041 tgcgctccgg ttatcccttc tttttacatc ctgtacatca catcaaatca ttaaacttgc
    14101 attaacttat ttataactta tattgtcaca tgcaaatcag catagtttcg aatgaaagtt
    14161 aatgcgaggt ggttatggct tctactttta ccagcgacac attgcctgcc gatcacaaag
    14221 cagctatccg tcagatgaag cacgcgctgc gggcgcagct tggcgacgtc cagcagatct
    14281 ttaatcagct aagcgatgac attgccacgc gagtggctga aatcaacgca ctcaaagcac
    14341 agggcgatgc cgtctggccg gtgctgtctt atgccgatat caaagcaggt catgttactg
    14401 cagagcagcg cgaacagatt aaacgtcgcg gttgtgcggt gataaaaggc catttccccc
    14461 gcgaacaagc gctaggctgg gatcagtcga tgctggacta tctggaccgc aaccgctttg
    14521 acgaggtcta caaaggcccc ggcgataatt tcttcgggac gctcagcgct tcacgtcccg
    14581 agatttaccc catctactgg tcgcaggcgc aaatgcaggc ccgccagagt gaagaaatgg
    14641 cgaatgcgca gtcgtttctc aatcgtctgt ggacatttga aagtgatgga aagcaatggt
    14701 ttaacccgga tgtgagcgtc atctaccctg accgtatccg ccgccgtccg cccggaacga
    14761 cctccaaagg tcttggagcg cataccgact ccggggcgct ggaacgctgg ctgcttccag
    14821 cgtatcagca cgttttcgct aacgtcttta atggcaatct ggcgaaatac gatccctggc
    14881 atgcggcaca tcgtacggaa gttgaagagt acacggtgga caacaccacc aaatgttccg
    14941 tgtttcggac attccagggc tggacagcgc tctctgatat gctgcctagt caggggttgc
    15001 tgcacgtcgt gcccattcct gaagccatgg cgtacgtact gttacgtccg ctgcttgatg
    15061 atgtgccgga ggatgaactg tgcggcgtag cgcccggaag agtgttgccg gtatcagagc
    15121 aatggcatcc actgttaatt gaggcgttaa ccagcattcc aaaactcgag gccggagact
    15181 ccgtctggtg gcactgcgac gtcatccatt ccgttgcccc cgttgaaaat caacagggtt
    15241 ggggcaacgt gatgtacatt cctgcggcac cgatgtgcga gaaaaatctt gcctacgcgc
    15301 acaaggtgaa ggccgcactg gaaaaaggcg catcgccggg cgacttcccg cgcgaggact
    15361 atgaaacaaa ctgggaagga cgctttacgc ttgccgacct caacattcac ggtaagcgag
    15421 cgttgggcat ggatgtttga gagttaattg aagtgaattg atgtgggatt aagaaaatcg
    15481 attcgacgtt actcagattg ctgacaagtg cgctttctta atgcctgata agcatagcgc
    15541 atcaggcaat cttgcgtttg tcatcagccg ccgtcacccc gacagtatcg gggtgacgga
    15601 taataactta ctcgatccct ttactacgca ggtaatcttc gtaattaccg ctaaagtcga
    15661 tcacgcgttc cggggtgatt tccagaatgc gggtcgccag ggagcttacg aactcacggt
    15721 cgtgtgaaac aaagatcagc gtgccctgat acagttccag cgccatgttc agtgactcga
    15781 tggattccat atccaggtgg ttggtcggtt cgtccatgat cagaatgttc ggcttctgca
    15841 tcattaactt accaaaaagc atccgccctt tttctccacc ggaaagcact ttagcaggct
    15901 ttttgatgtc gtcctggctg aacagcaaac gaccgagaat actgcgcacc gcctgctcgt
    15961 catcgccttc ctgcttccac tggctcatcc attcgaacac ggtcagatca ttttcaaact
    16021 catattcgtg gtcctgagca tagtaaccaa tgcgcgcgtt ttcagaccat tttacggtgc
    16081 cgctgtccgg ttgcagatcg cccaccagcg ttttcaacag cgttgattta ccgacgccgt
    16141 tggtacccag taccgccagt ttttcaccga cttccagcag caggttgaga tttttaaaca
    16201 gcggaccgtt atcaaaccct ttggtcagac cttccacttc cagcgcgtta cggaacagct
    16261 tcttatcctg ttcaaaacgg atgaacgggt tctgacggct ggaggctttc acctcttcca
    16321 gtttgatttt atcaatctgg cgcgcgcgcg aagttgcctg gcgagatttc gaggcgttgg
    16381 cgctaaagcg gctaacgaaa gattgcagct cagcaatctg cgctttcttc ttggcgttat
    16441 cggccagcag acgttcacgc gcctgggtcg ccgccgtcat gtactcatcg tagttacccg
    16501 gataaacgcg cagctcgccg taatccagat ccgccatgtg ggtacagacc atgttaagga
    16561 agtgacggtc gtgcgagatg atgatcatgg tgctgtcacg ctcgttcagc acctgttcca
    16621 gccagcgaat ggtgtcgatg tcgaggttgt tggtcggttc gtcgagcagg agaatatccg
    16681 gatcagcaaa cagcgcctgc gccagcaaca cacgcagctt ccagccagga gcaacttcac
    16741 tcatcgggcc gtagtgctgt tccactggaa ttcccacgcc aagcaacaat tcaccggcac
    16801 gagcttccgc agagtaaccg tccatttcgc cgtatttaac ttccagatcg gccactttat
    16861 agccgtcttc ttcactcatt tccggcaaag catagatgcg gtcgcgctcc tgcttcactt
    16921 cccacaactc tttatgcccc atgatcaccg tatccagcac agtgaactct tcaaaggcaa
    16981 actgatcctg acgcagttta ccaatgcgct cgttgggatc gagggaaacg ttacccagcg
    17041 tcggctcaag gtcgccgccg aggatcttca taaaggtgga tttaccacta ccgttcgcgc
    17101 caatcaggcc gtaacggttg ccgccgccaa atttgacgga aatgttttca aacaacggct
    17161 tactgccgaa ctgcatggtg acgttactgg aaactaacac aggcgtatcc tgaaaagaga
    17221 tatgacaaac cgcgcattat gccataagtc tgaaagaaga tcctgctcct cgcttaagat
    17281 ctacactcaa gccaaaggca aaaaatgtga tttcgtacac atctgatttc actgtgagct
    17341 ggaatgaact tataatgcgc ttccaatact ctaatattct caacccaatg gcctgccagg
    17401 cacaaaatct cgcttaacat gaatatgaaa ttgaaaacat tattcgcagc ggccttcgct
    17461 gttgtcggct tttgcagtac cgcctctgcg gtaacttatc ctctgccaac cgacgggagt
    17521 cgcctggttg gtcagaatca ggtgatcacc attcctgaag gtaacactca gccgctggag
    17581 tattttgccg cggagtacca gatggggctt tccaatatga tggaagcgaa cccgggtgtg
    17641 gataccttcc tgccgaaagg cggtactgta ctgaacattc cgcagcagct gatcctgccg
    17701 gataccgttc atgaaggcat cgtcattaac agtgcagaga tgcgtctgta ttactatccg
    17761 aaagggacca acaccgttat cgtgctgccg atcggcattg gtcagttagg caaagatacg
    17821 cctatcaact ggaccaccaa agttgagcgt aagaaagcag gcccgacctg gacgccgacc
    17881 gccaaaatgc acgcagagta ccgcgctgcg ggcgaaccgc ttccggctgt cgttccggca
    17941 ggtccggata acccgatggg gctgtatgca ctctacatcg gtcgcctgta cgctatccat
    18001 ggcaccaacg ctaacttcgg tatcggtctg cgtgtaagtc atggttgtgt gcgtctgcgt
    18061 aacgaagaca tcaaattcct gttcgagaaa gtaccggtcg gtacccgcgt acagtttatt
    18121 gatgagccgg taaaagcgac caccgagcca gacggcagcc gttacattga agtccataac
    18181 ccgctgtcta ccaccgaagc ccaatttgaa ggtcaggaaa ttgtgccaat taccctgacg
    18241 aagagcgtgc agacagtgac cggccagcca gatgttgacc aggttgttct tgatgaagcg
    18301 attaaaaacc gctccgggat gccggttcgt ctgaattaat cttcaaaacg taaaacaaag
    18361 gcggactcat aatccgcctt ttttatttgc cagaccttac caggccggga tcataaccaa
    18421 caaaacatat cccaccagcg ccgcccacaa cagcatcggt attgaataga gatggaaacg
    18481 ccaccagatg cggcgatcgt tcgccatacg tagcgcaatc aaatttgcca gcgatccggg
    18541 taataaccca aagccaccta cgtttaccgc ccatgccagt aataaagacg gcggcacata
    18601 gttcagcaac aatatagtac tcggcacgtt actgatcacc tgcgataaac cgattgccgt
    18661 taaccataat ccgggttcag ataaatgact cacgttaccc aacacgcctt gcaacgctgg
    18721 aagctgggtc agtaaatgga cgtcgataaa catcgccata aacaccagca gcagcgtcca
    18781 gtccacactc aacaccacgc gacgcgccag cagcgcaaag cccgccgcca caatcaccag
    18841 tccccacagc tcttgtttga actccagcgc cgtcagaaag acgatataca gccccagaca
    18901 actccacacc agccgcggtt tccactccgg tgtttgcacc cccgtatggt attggagtgc
    18961 ctttccaggg aaacaacacc agcacaacag caggagcgtc agcatcattg cgccagccag
    19021 cggtgccatt tgggcaataa atccggcaaa cgaaagacca gaacgtcccc agataagaat
    19081 attttgcggg ttgccaattg gcgtcagtag cgaaccagcg ttgactgcca gcgcctcaaa
    19141 aataatcagc cgattaaccg ggatctcaca cagccttttt agcgtgatag tcagcggaac
    19201 aacaataaac agcgtgacat cgttggtcag aaaggtagaa agcagcgccg ccgccagcac
    19261 cataaacatc gccagccgac gctccgtagc aaagcggcgc accattttgc gccccagcac
    19321 atcaaaataa ccgcttaact ccacaccttt ggtcagcagc atcaggccgc ttaaggtgat
    19381 gatggtgtgc cagtcgatag cagcaggcca ggatttcggt gcaaagggca cgaaaaaact
    19441 taatccgata ccaacaagaa ttaataactg aaaaaaacga tcgccttgca gcgtgcgtaa
    19501 aaaaggcagg ctcatttggc accgtgtttc tgggtaaaca aacgaaaggc atccagcgtc
    19561 tcttcactaa catggtgctc catgccttcc gcgtcgcgac gggcgatttc cggactgacg
    19621 cccaacacca acaagaaatt ttcgactatc tgatgtcgct cgcggctttc ctgcgccagc
    19681 ttctctcctt ctgccgttaa aaacacgcca cgccagggga tcatttcaat cagccccata
    19741 gttgccagtc gcttaagcat tttagccacc gtcggttgcg aaactcccaa acgagcagcc
    19801 atgtctacct gacgagcttc ccccacttcc ctgatcaagt cagaaatcag ctcaacgtaa
    19861 tcatcaataa gctcgcgccg atgcgcctct ctgacctggc ggaacccttc aacgtgctct
    19921 tcctcgttca ctaactgcgt cacttttttt gctgttggcg tacctgcgcg acgactcatt
    19981 gtgcttcctc attatggtga cgcatacata gcgttctcat ttcgtgcgca cattgtaaac
    20041 cagagttgcg aaggtacaaa aaattaacgt tttagcaata gctatataat atagcctgtg
    20101 ctatatctgt atgtaatgca atcatccctc aaggatcgac gggattagca agtcaggagg
    20161 tcttatgaat gagttcaaga ggtgtatgcg cgtgtttagt cattctccct ttaaagtacg
    20221 gttaatgctg ctctctatgt tgtgcgatat ggtcaacaac aaaccgcagc aagataaacc
    20281 ttccgataaa tagcggcgtc gcggtacgcc gcttcactcc tgctttcatg caggcacgac
    20341 gcgttttggt ctgaaaaacc ccactttttg tcggatttgc aatccccttc gcaaaagatt
    20401 tgttcgtcag tagttgacct gaactgcggc tcgctctatc ttcttgcagc cctgcgtata
    20461 ttgcggctcg cggatgcgga cccctttccc ctcttcacgc actcttgcag gtattgaccc
    20521 ttgacgccag ggtaagcaca tggcgtttgt tacgatagtg gcatatgaat ttaaccctca
    20581 aagaatcgct tgttacccgt agccgggtat ttagcccgtg gactgcgttc tactttttac
    20641 agtcgctatt aattaacctc ggcttaggtt accccttcag tttgctctac accgctgcgt
    20701 ttacggctat tttgcttttg ctatggcgaa cattgcctcg cgtacaaaaa gttctggtcg
    20761 gtgtcagttc gctggtggcg gcttgttatt tcccttttgc tcaggcctac ggcgcgccta
    20821 atttcaatac attgctggca ttgcactcca ccaatatgga agagtcgacc gaaatcctga
    20881 cgatttttcc gtggtacagc tacctggtcg gcttatttat ttttgcgctc ggcgtaatag
    20941 caatcaggcg aaaaaaagag aatgaaaaag cgcgctggaa taccttcgac agcctgtgtc
    21001 tggtattcag tgtggcgaca ttttttgttg ctcccgtgca aaacctggcc tggggtggcg
    21061 tatttaaact gaaagatact ggctatccgg tatttcgttt tgctaaggat gtcatcgtca
    21121 ataataacga ggtgattgaa gagcaagaac ggatggcaaa actttccgga atgaaagata
    21181 cctggacggt cactgccgtt aagccgaagt atcagaccta tgtggtggtg atcggtgaaa
    21241 gcgcgcgtcg cgatgccctc ggtgcctttg gcggtcactg ggacaatacc ccgtttgcca
    21301 gcagcgttaa cggtttgata tttgctgact acattgccgc cagtggctcc acgcagaaat
    21361 cgcttggctt aacgctcaat cgcgtagtcg atggcaaacc acagtttcag gataactttg
    21421 tcaccctggc aaatcgcgcg ggcttccaga cctggtggtt ttccaaccag ggccaaatcg
    21481 gcgaatacga tactgctatc gccagcatcg ccaaacgtgc agatgaagtg tacttcctga
    21541 aagaaggtaa ttttgaagca gataaaaaca cgaaagacga agcgttactg gatatgaccg
    21601 ctcaagtgct ggcgcaagag cactcgcaac cgcagctgat tgttctgcat ctgatgggct
    21661 cgcatccgca ggcctgcgac cggacaaaag gcaaatacga aacctttgtg caatcgaaag
    21721 aaacgtcgtg ctatctctat actatgacgc aaacggacga tttactgcgc aagctgtacg
    21781 atcagttacg caacagcggc agcagcttct cgctggttta cttttctgac cacggtctgg
    21841 cctttaaaga gcgcggcaaa gacgtgcaat accttgccca tgatgataag tatcagcaaa
    21901 atttccaggt gccttttatg gtcatttcca gcgacgataa agcgcatcgg gtgattaaag
    21961 cccgccgctc agccaatgac ttcttaggct ttttctcgca gtggacggga attaaagcga
    22021 aggaaattaa catcaaatac ccgtttatat ctgagaagaa agccgggccg atatacatca
    22081 ccaacttcca gttacagaag gtggattaca accatctcgg aaccgatatt ttcgacccga
    22141 agccttaaaa acaaaaatcc gccccgagag gcggattttt tatatcacca aagtgattag
    22201 aagcggtaac caacaccggc aatccaggtg cctacgtcaa cgctacgaat acggctctgc
    22261 tcgtaagaga agtccagagc aacgttttcc atcgggttga actgcagacc agcaccgtag
    22321 gagaaaccgt agtcgctggt gtcgtgtttg taggtcgggt attcagtggt ctggaattta
    22381 ccataaccca cacccactac accgtagatg cttgcccagt cgttaatgcg gtaagccgga
    22441 ccagcagtga tgccgtagta ctggtttttg ttgtagtcac cagagcttgc agtacggctt
    22501 ttctcggtgt aagtgaatga accgatcaca cccagcgggc tgttgtcttc ttcatagcgg
    22561 tatttcaggt tgaaaccgcc cattttgttc atttggccct gagcgtcgct ctgtgcgtaa
    22621 ccgccagtta cggtagaagt cgcagctacg gaagtacctg cggtgaaagc cagaactgcg
    22681 gccagtgctg aaagacatgc aatttttttc ataaccacct caaatgtgat tcaaataagt
    22741 cctaagtttt aaatatatca aaaattaatg ggaaactctt cgcgatttgt gatgtctaac
    22801 gggccatttc atgtaacaga acgtttccat acaccgctat ccatctaaat ttaaatcact
    22861 ttttcagaga actgcgtaag tattacgcat gttttccctg tcattcatcc agattattcc
    22921 taatcaccag actaatgatt ccatcaatcc tggcgcattt tagtcaaaac gggggaaaat
    22981 tttttcaaca aatgctcaac cagcattggg tatatccagt acactccacg ctttacttaa
    23041 gtctagatat ttgtgggaga aaggatgcct ggttcattac gtaaaatgcc ggtctggtta
    23101 ccaatagtca tattgctcgt tgccatggcg tctattcaag gtggagcttc gttagctaag
    23161 tcactttttc ctctggtggg cgcaccgggt gtcactgcgc tgcgcctggc attaggaacg
    23221 ctgatcctca tcgcgttctt taagccatgg cgactgcgct ttgccaaaga gcaacggtta
    23281 ccgctgttgt tttacggcgt ttcgctgggt gggatgaatt atctttttta tctctctatt
    23341 cagacggtac cgctgggtat tgcggtggcg ctggagttca ccggaccact ggcggtggcg
    23401 ctgttctctt ctcgtcgccc ggtagatttc gtctgggttg tgctggcggt tcttggtctg
    23461 tggttcctgc taccgctggg gcaagacgtt tcccatgtcg atttaaccgg ctgtgcgctg
    23521 gcactggggg ccggggcttg ttgggctatt tacattttaa gtgggcaacg cgcaggagcg
    23581 gaacatggcc ctgcgacggt ggcaattggt tcgttgattg cagcgttaat tttcgtgcca
    23641 attggagcgc ttcaggctgg tgaagcactc tggcactggt cggttattcc attgggtctg
    23701 gcggtcgcta ttctctcgac cgctctgcct tattcgctgg aaatgattgc cctcacccgt
    23761 ttgccaacac ggacatttgg tacgctgatg agcatggaac cggcgctggc tgccgtttcc
    23821 gggatgattt tcctcggaga aacactgaca cccatacagc tgctggcgct cggcgcaatt
    23881 atcgccgct
//
LOCUS       DBFIFI010000055        23851 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.055, whole genome shotgun sequence.
ACCESSION   DBFIFI010000055 DBFIFI010000000
VERSION     DBFIFI010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23851
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.055"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(76..1341)
                     /locus_tag="N7B39_002082"
     CDS             complement(76..1341)
                     /locus_tag="N7B39_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU2422066.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALVVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGS
                     YKGQPVTQLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQDVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANRERFISPFEYAKINNPLKQ"
     gene            complement(1539..1614)
                     /locus_tag="N7B39_002083"
     tRNA            complement(1539..1614)
                     /locus_tag="N7B39_002083"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1579..1581),aa:Phe,seq:gaa)
     gene            complement(1720..2427)
                     /gene="yqgA"
                     /locus_tag="N7B39_002084"
     CDS             complement(1720..2427)
                     /gene="yqgA"
                     /locus_tag="N7B39_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HGU2422067.1"
                     /translation="MVIGPFINASAILLGGMLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            2580..2663
                     /locus_tag="N7B39_002085"
     CDS             2580..2663
                     /locus_tag="N7B39_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2422068.1"
                     /translation="MHAISLHSLAYRRFARNPSLFMLLLMR"
     gene            2825..4960
                     /gene="speC"
                     /locus_tag="N7B39_002086"
     CDS             2825..4960
                     /gene="speC"
                     /locus_tag="N7B39_002086"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HGU2422069.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSR
                     SGILTLLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENIFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGIDAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGDVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(5010..6266)
                     /gene="nupG"
                     /locus_tag="N7B39_002087"
     CDS             complement(5010..6266)
                     /gene="nupG"
                     /locus_tag="N7B39_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HGU2422070.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(6468..7547)
                     /gene="mltC"
                     /locus_tag="N7B39_002088"
     CDS             complement(6468..7547)
                     /gene="mltC"
                     /locus_tag="N7B39_002088"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HGU2422071.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGGTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(7612..7887)
                     /gene="yggX"
                     /locus_tag="N7B39_002089"
     CDS             complement(7612..7887)
                     /gene="yggX"
                     /locus_tag="N7B39_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HGU2422072.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(7915..8967)
                     /gene="mutY"
                     /locus_tag="N7B39_002090"
     CDS             complement(7915..8967)
                     /gene="mutY"
                     /locus_tag="N7B39_002090"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HGU2422073.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            9128..9847
                     /gene="trmB"
                     /locus_tag="N7B39_002091"
     CDS             9128..9847
                     /gene="trmB"
                     /locus_tag="N7B39_002091"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HGU2422074.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMIEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            9847..10173
                     /gene="yggL"
                     /locus_tag="N7B39_002092"
     CDS             9847..10173
                     /gene="yggL"
                     /locus_tag="N7B39_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HGU2422075.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERNLDEVRTSEL
                     FDVWWD"
     gene            10357..11076
                     /gene="yggN"
                     /locus_tag="N7B39_002093"
     CDS             10357..11076
                     /gene="yggN"
                     /locus_tag="N7B39_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HGU2422076.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            11252..12298
                     /gene="ansB"
                     /locus_tag="N7B39_002094"
     CDS             11252..12298
                     /gene="ansB"
                     /locus_tag="N7B39_002094"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HGU2422077.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDDVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFIASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            12415..13422
                     /gene="yggM"
                     /locus_tag="N7B39_002095"
     CDS             12415..13422
                     /gene="yggM"
                     /locus_tag="N7B39_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HGU2422078.1"
                     /translation="MKKQWIVGTALLMLMTGNVWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWTVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLETALN
                     IQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREDAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWITLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(13665..14801)
                     /gene="hemW"
                     /locus_tag="N7B39_002096"
     CDS             complement(13665..14801)
                     /gene="hemW"
                     /locus_tag="N7B39_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HGU2422079.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     NDLDNDVAYAQGREVKTIFIGGGTPSLLSGSAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEPAPRVEFSAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(14794..15387)
                     /gene="rdgB"
                     /locus_tag="N7B39_002097"
     CDS             complement(14794..15387)
                     /gene="rdgB"
                     /locus_tag="N7B39_002097"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035870 - dITP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0036222 - XTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HGU2422080.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTELGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDVLGGAPGIYSACYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            complement(15395..15685)
                     /gene="yggU"
                     /locus_tag="N7B39_002098"
     CDS             complement(15395..15685)
                     /gene="yggU"
                     /locus_tag="N7B39_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HGU2422081.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEVAALIN"
     gene            complement(15682..16248)
                     /gene="yggT"
                     /locus_tag="N7B39_002099"
     CDS             complement(15682..16248)
                     /gene="yggT"
                     /locus_tag="N7B39_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HGU2422082.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            complement(16266..16970)
                     /gene="yggS"
                     /locus_tag="N7B39_002100"
     CDS             complement(16266..16970)
                     /gene="yggS"
                     /locus_tag="N7B39_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HGU2422083.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            16988..17968
                     /gene="yggR"
                     /locus_tag="N7B39_002101"
     CDS             16988..17968
                     /gene="yggR"
                     /locus_tag="N7B39_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HGU2422084.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLTENQRLRGSAFAQRHGISLALRLLPSHCPQ
                     LEQLGTPPILPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVGEGRL"
     gene            complement(18159..18575)
                     /gene="ruvX"
                     /locus_tag="N7B39_002102"
     CDS             complement(18159..18575)
                     /gene="ruvX"
                     /locus_tag="N7B39_002102"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HGU2422085.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKIDSASAVIILESYFEQGY"
     gene            complement(18575..19138)
                     /gene="yqgE"
                     /locus_tag="N7B39_002103"
     CDS             complement(18575..19138)
                     /gene="yqgE"
                     /locus_tag="N7B39_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HGU2422086.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            complement(19250..20197)
                     /gene="gshB"
                     /locus_tag="N7B39_002104"
     CDS             complement(19250..20197)
                     /gene="gshB"
                     /locus_tag="N7B39_002104"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HGU2422087.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQ"
     gene            complement(20210..20941)
                     /gene="rsmE"
                     /locus_tag="N7B39_002105"
     CDS             complement(20210..20941)
                     /gene="rsmE"
                     /locus_tag="N7B39_002105"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HGU2422088.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            complement(21021..21728)
                     /gene="endA"
                     /locus_tag="N7B39_002106"
     CDS             complement(21021..21728)
                     /gene="endA"
                     /locus_tag="N7B39_002106"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HGU2422089.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDHYNLTLSRQQTQLFNAWDKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            complement(21823..22320)
                     /gene="yggI"
                     /locus_tag="N7B39_002107"
     CDS             complement(21823..22320)
                     /gene="yggI"
                     /locus_tag="N7B39_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HGU2422090.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYSCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            complement(22397..23791)
                     /gene="galP"
                     /locus_tag="N7B39_002108"
     CDS             complement(22397..23791)
                     /gene="galP"
                     /locus_tag="N7B39_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HGU2422091.1"
                     /translation="MPDAKKQGQSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAGLNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
BASE COUNT         5868 a         6003 c         6254 g         5726 t
ORIGIN      
        1 ctaataccgt tttgtctgtt tttttaacaa agaaaataat cctggagtag gttcccgggc
       61 atttgcccgg gatgattact gttttaatgg attattaatc tttgcatatt caaatggact
      121 gataaatctt tctctgtttg catccagaaa atcggcccac cactgtagca tcagtcgccg
      181 ttcttccaga tgttctgctt tatgaatgta agcagcacgt acaccgttac gttcctgatg
      241 gctcatctga cgttcaactg catcgcggga ccacaaacct gattctatta acgcactaca
      301 tgccatcgcc cggaatccat gaccacagac atcctgggtt gtgtcatacc ccataaccct
      361 cagggcttta tttacggtgt tttcactgat tggtttacgt gggtcatgtg ctcccgtaaa
      421 gatcagacca ttttcaccag cccatgtctg aagttctgcc agtatggcta caacctgttt
      481 gctgagtggc acaagatgtg gtgttcgcat cttggatccc cggtgtgaga attttacgcc
      541 tggcagaggt tcgcgttcgg ctggaatagt ccacatggca ttgtcaatat caatttcaga
      601 ccagcgggcg tatctgagtt cactggaacg aataaaaacc agtaaattca gcataacggc
      661 aagctgggtg acaggttgcc ccttgtaact gccaatacgc gacaaaagtt caggcaggcg
      721 agaaagatca agagcggggc gatgctggcg ttttaccgta gtcaatgcgc cagccatatc
      781 cagagcagga ttatatctga tgatgccaga ctgtacggca taacgcatta cggcagatat
      841 tctttgctgc agacgactgg cgatttcata aatttgttta gcttctgcag cacgaacagg
      901 gataagcagg tccggggtat tgagtgttgt gatatcccga ttgccgataa aggggaatac
      961 gtgtgtctcg aggcttttga gtactttatc gctgtgtgat tgtgcccatt gtttattact
     1021 ggcatgccag cgtcgtgcaa tatgttcgaa agagagttgt aaatcaggga ctactttttt
     1081 agctcttttc tgttcgcaag ggtcaacccc ctccgaaatc agttttcggg cctcatcccg
     1141 ttttgtacga gcttcggaga gagatacttc tggatacaca acaagtgcca gagtcttctc
     1201 tttacccaga atacgataac ggagacgcca gtaacgggaa ccattaggat gtaccagcag
     1261 gaacatgcca gccccgtcag tgagtttata agccttgtca gtgggctttg cagcccggat
     1321 ttttgcgtca gtcagtgcca tagttcagcc ttctgaatgc gttaaagagg gtacaaattc
     1381 tgtcgaactg gggtatgccc gcaattatac ccacacaatg aggttgatgt ggggagaatt
     1441 tggttgagtt cggtagaatt gatttggagg aagaacactt aaatcatggt gttctgaaac
     1501 gaaaaacgga cctccgtgga ggtccgttga tatgaatttg gtgcccggac tcggaatcga
     1561 accaaggaca cggggatttt caatcccctg ctctaccgac tgagctatcc gggcaacggg
     1621 gcgcattaaa cctgattcgc ctcgtctcgt caatcaaatt tcttcaattt actgcagact
     1681 gcacaatcta tcatcacttt gccgctattg cacacattgt caggcaaacc aggcggtcca
     1741 ggcggcggaa agcggcattg ccagaaggag tgctggaagc atgttgacca ccgggaacat
     1801 tttaatgcca cagatgcgta atccggttgc cagcagcaat aaaccgccta cggcgctgaa
     1861 gtctgccatc atcgacggtg tggtcagcgg taatatcagc gcggcagccc acgccagcgt
     1921 taactggatg atcagtaatg ggatactaat caccgatacc gcaataccca gcgagcaggc
     1981 gaagatcatc gccgtaaaga aatcaagaaa tgacttggcg attaaaatac tcggatcgcc
     2041 ggtcatccct tcgttcatcg ccccgaagat cccggtgccg ctggcgcaaa acaggacaat
     2101 aatcgcgaca taattctgaa taaaagattc atgcgctggc ttcttacgtg agtgacgaaa
     2161 cagattttgt gctttggcga ccgctgtatt gacgcctttc tccagcagac aaatttcgcc
     2221 gattaatgct ccgagtaagg ttgctaaaac catcgccgga aggttggcgc atttcaccac
     2281 cagtaaaata ccaatcccca gcgatgccaa accaaaaatt gatgtcatgg agacgcggat
     2341 acgttccggt aagcgttggc taagcagtgc accgagcatg ccacccagta atattgcact
     2401 tgcgttgata aaagggccga tgaccacaag agttcctgtt aactgtattg tttgattttc
     2461 ttattatgaa ctttttgcct tgttgatggc ttgtctggtc gcaaagtcct ggcttgcacg
     2521 ccttagcgaa aggtgccatg attgcgcgaa ttttctcctc tctgtacgga gtttgcccga
     2581 tgcacgccat ctccttacat tctctcgctt atcgccgttt cgcgcgaaac ccttctcttt
     2641 ttatgctact gctcatgcgg tgaagttaac gcacgctcac tgcaggacaa cagtaaaatc
     2701 agagcctttc tgcttttact gatgtctggc ggtcggagct ggtgaccagt ttgacccata
     2761 tctcatgggg cagggtttta caccttgtcc agtattctta cttccccgaa acgggtttgc
     2821 gcttatgaaa tcaatgaata ttgccgccag tagtgaactg gtatcccgac tttctactca
     2881 tcgccgcgtg gtggcgttgg gagatactga ttttacggac gtcgcggcag tcgtcattac
     2941 cgctgcggat agtcgcagtg gcattcttac gttgcttaag cgcaccggtt ttcatctacc
     3001 ggtgtttttg tattccgaac atgctgttga attacctgcg ggcgttacgg cggtaatcaa
     3061 cggcaacgag cagcagtggc tggagctgga atccgcagcc tgtcagtatg aagagaattt
     3121 gctgccaccg ttttatgaca cgctgacgca gtacgttgag atgggcaaca gcacctttgc
     3181 ttgccctgga catcaacatg gtgcgttctt taaaaagcat cctgccggac gccattttta
     3241 cgatttcttt ggtgagaaca tctttcgcgc cgatatgtgt aacgctgacg taaaattggg
     3301 cgatctgctt attcatgaag gatcggcgaa agatgcgcag aaattcgcag ccaaagtctt
     3361 tcatgccgat aaaacctatt ttgtgctgaa cggcacatcg gcagcgaata aagtggtgac
     3421 gaatgcgctg ttaacgcgtg gcgatctggt gctctttgac cgtaacaacc ataagtcgaa
     3481 tcatcacggc gcgttgattc aggcgggggc gacgccggtc tatctggaag cttcacgtaa
     3541 cccgtttggt ttcattggcg gtattgatgc gcactgtttt aatgaagagt atctgcgcca
     3601 gcaaattcgc gacgttgcgc cagaaaaagc cgacctgccg cgcccgtttc gcctggcgat
     3661 tattcagctg ggaacctatg acggcactgt ctataacgcc cgtcaggtga tcgataccgt
     3721 tgggcatctg tgtgattaca ttctgtttga ttccgcgtgg gtcggttatg aacaatttat
     3781 cccgatgatg gcggatagct cgccgctgct gttagaactt aacgaaaacg atccggggat
     3841 ctttgtgact cagtcggtgc acaaacagca ggcgggattc tcacagacgt cgcagatcca
     3901 taaaaaagat aaccatatcc gcggacaggc gcgtttttgc ccgcataagc ggttgaataa
     3961 cgcctttatg ctccatgctt ctaccagccc tttctatccg ctgtttgctg cactggatgt
     4021 taacgccaaa attcatgaag gggagagtgg gcgtcggctg tgggctgagt gcgttgagtt
     4081 gggtattgag tcgcgcaagg ctattcttgc gcgctgtaag ctgttccgcc cgtttatccc
     4141 gcccgttgtt gatggcaaat tgtggcagga ttatccgaca tcagtgttag ccagcgaccg
     4201 tcgttttttc agttttgagc cgggggcgaa gtggcacggc tttgaaggat atgccgcgga
     4261 tcagtatttt gttgatccgt gcaagctgtt actcactaca ccaggtatcg atgccgaaac
     4321 cggcgaatat agcgactttg gcgttccggc gacgattctg gcgcactatc tgcgtgagaa
     4381 cggcattgtg ccggagaagt gcgatctcaa ttccattctg ttcttattaa ctccggcgga
     4441 aagccacgag aagctggcac aactggtggc gatgctggct caatttgaac agcatattga
     4501 ggatgactcg ccgctggctg aggtgttgcc gagcgtttat aacaaatatc cggtgcgcta
     4561 tcgcgactac accctgcgcc agttgtgtca ggagatgcac gatctgtatg tcagtttcga
     4621 cgtcaaagac ctacaaaaag cgatgttccg ccagcagagt ttcccgtcag tggtgatgaa
     4681 cccccaggat gcgcatagcg cttatattcg cggtgacgtg gagttggtgc ggattcgtga
     4741 tgccgaaggg cgaattgcgg cagaaggggc gttgccttat ccacctggcg tgctttgcgt
     4801 ggtacccggg gaagtctggg gtggggcggt tcaacgttat ttccttgcac tggaagaagg
     4861 ggtgaatttg ttgccgggat tttcgccgga gctgcaaggt gtttatagcg aaaccgatgc
     4921 ggatggcatg aaacggttgt acggttatgt gttgaagtaa gaataaaaaa aacgggtcgc
     4981 cttctggcga cccgtttttc tttgcgtaat tagtggctaa ccgtctgtgt acctgtcggg
     5041 acacgaacgt gtttatattt gaacatcgcc atgaacgcga aggccagaac cacggagtaa
     5101 ccagcgaaaa tcaaccatac ggtctgccag tcggtaatac cgttttgggt gtacatctca
     5161 acaactttac cgctcacgat gccgccgagg atacagccga agccgttagt catcatcagg
     5221 aacatccctt gtgcactggc gcgaattgcc gggctaactt ctttttcgac aaacaccgaa
     5281 ccagagatgt tgaagaagtc gaacgcacag ccgtaaacaa tcatcgatag aaccagcagt
     5341 acggtgccga acggagtcgg gtcgccgtaa gcaaacagcg caaaacgcag gatccacgcc
     5401 acaatactga tcatcattac gttcttaata ccgtagcggc ttaagaagaa cgggatggtc
     5461 agaatgaaca gggtttcaga gatctgcgaa atcgacatga tgattgacgc atgctgcaca
     5521 ataaagctgc tggcaaacat cggatctttg tcgaagctgt gcaggaaggt attaccgaac
     5581 atgttggtaa tctgcagttc cgcgcccagc agcattgaga agatgaagaa gattgccata
     5641 cgcttgtttt taaacagcgc gaatgcatcg aggcccagca gggttgtcca gctctgattc
     5701 gcttgctgtt tagcaaccgg aatatgcggc agagtcaggg taaacagaac cagaatggcg
     5761 gaaagtgctg cgccaatata cagctgcatg tggcttaatt cgaagccaga caggctcacc
     5821 acccacattg ccatgataaa gccgatggtg ccccagatac ggattggcgg gaagtcagta
     5881 acgatatcca tcccggcatt ttgcaggcga tagtaagaga tggtgttgat taacccaagc
     5941 gttggcatat aagcaaacga gttaatcaat atcacaagga acatcgcctc cggcgtcgtg
     6001 acctgtgccg ccatgaacag cgtgatagcg ccaatggtgt ggcaaatggc atatacccat
     6061 ttcgcactta accatttgtc ggccacaatc cccagcagcg caggcataaa gaccgcggcg
     6121 atacccagtg agctataaac tgcgccaata gaagcaccgt caaacttcag ggtaacaaac
     6181 atataggaac cgagggtcgt cagccaactt ccccacagac agaactgcag aaaagagagg
     6241 attttcagct gcagcttaag attcatgtta atttcctcac atcgtgatgc ggatgagtgt
     6301 ttgaaaaagc acatttgacc gtgcgagatg atgggattct atcaacttcg cggactggtt
     6361 ttttgttacc tgtggcaaat aattgcaaac attcctaatt tgcaaaacga aattgtgata
     6421 tggataacat ttttgcccct gagcatcgtc aggggcggtt agtggaatta tcggcggcgg
     6481 taggattttt gcgcggtatt cactttataa agataacggc gagattccgc agagggatgg
     6541 cgggtcgtca gcgtttgata aacatcgccc ggcgtcatgg tgttaataat attagccgcc
     6601 tgaatcttat cattcgaaaa gactcgcagc acgctgcctg cgccgccgtt ataggcggtg
     6661 atgacggcat aacgccggga tgtagggtta tcaattccgc cgagataaac attgttcagc
     6721 atcgccagat acgcggtgcc ggtatcaata ttgctggcag gatcaaacaa gaaactgcgg
     6781 ctcggcgtgc cggatttccc ctgcgagcgg aacacatctt tcccggcagt atgttgtacc
     6841 acctgcatta accccagcgc atcggaacgg ctgaccgcat acgggttaaa ggaagattca
     6901 gtctgcataa ttgccagaat cagcgactca tcaacgccat atttccgtga cgcctggcgg
     6961 accatgccga gatatttgtg cgcacgttta tcaaggtggt tcggcaccat gttaatggtg
     7021 acgctgtaga tgatacgcag cccgttgctg cggctcttca gacggttttt cagcagataa
     7081 tccgcgaagt tgcttgcgcg accttcccag cgaatcggct gcccggtgtt gtccaccacc
     7141 tgaccgtaaa ggaaaggttc tttcgaaatt gtaatatcat caacgtcgga atagagatcg
     7201 accgaactcg gatcgtcacc catcagcaac gttttgataa ttgcccggcg caaatgcgcg
     7261 gcaggttctg tcccggcgat ggtttcgata gtaatcgtac cgtcatcgaa gttgatgtgg
     7321 ctgcgggtct gatattgatc ggtgtatttc acgtagtcct taggaccggc gatcaccacc
     7381 tctttgaatc cccagatgtt ctcaatattg tgggcaaatt gccccatcag aatatcaaaa
     7441 ccgttggtgt ctttgaccca ggcttcgtta taggtaccgc cttttttggt cgtcgaacag
     7501 gagatgagca acggcgcaat caaagccagc gcgagatatt ttttcatcat tccgggtgcg
     7561 tgttgtgttt gtttttatgc aagctgccgg aggcgtgctc cggcactgtt tttatttttt
     7621 atcttccggc gtatagccct cgatatgcac ctctttaccc tcgaacagga agttgaccat
     7681 ctcctgctcc agcagcttgc ggtgctcggc attcatcatg ttgagtttct tttcattaat
     7741 cagcatggtt tgtttgtgct gccactgcgc ccaggcttct ttggagatct cgttatagat
     7801 gcgttttccc agttcgccgg ggtacagctg aaaatcctga ccttctgctt cacgttgcag
     7861 gaaagtacaa aaaatcgttc tgctcataaa tcatcctctt tatcgactca cgcgctaaac
     7921 cggcgcgcca gtgcgtaact gctgtaacaa acgctccacg ggagccgcca ggccaactga
     7981 cggcggttgc gctaagttat accagagcgc attgccttca tccatgcagc cggtgaatga
     8041 cgacacggga agccacatag gcacaatatc taagtggaaa tggctgaagg tatgccgaaa
     8101 cgcggtcagc tgcgtcaggt tatcggcagc aatctgccgt tgcgccagcc actgccgcaa
     8161 actttcttcg tcggcaaact gcgggaaaca gtataaaccg ccccacaatc cgctcggcgg
     8221 acgctgcgcc agcaatactt catcttcgtg ctgtaacagg agaaagtagc cggtgcgctc
     8281 cggcagcgtc tgtttcggtt ttttgcccgg ataaagcgac cagctattgt tggtggcggc
     8341 aatacatccg ttttgtagcg gacagagcga acatttcggc ttcgaacgcg tgcaaatcat
     8401 cgcgcccaaa tccatcatcg cctgattaaa ccgttccacg ccgaccgcgg gcgtcacctg
     8461 ctcgcttaaa ctccataatt tattctcgac ctcttttttc ccaggccagc cgcttacagc
     8521 atagcagcgc gccagcaccc gtttgacgtt accgtcgaga atcggaaagt gcttacccag
     8581 agaaagcgat agaatcgcgc ctgcggtgga acgcccgacg cccggcagtg ctgcaacttc
     8641 ctcaaaggtt tccgggaatt taccgccgtg taaggtcgcc acttgttgtg ccgctttatg
     8701 cagattgcgc gcgcgggcgt aatagccaag ccccgtccac aagtggagaa cttcgtcgag
     8761 cggcgcattg gcgagatcgg tcaccgtcgg gaagcgcgcc ataaagcgtt caaaataggg
     8821 gataacggtc gcaacctgag tttgttgcaa catcacttct gagagccata ctttgtaggg
     8881 cgtcttgtca atttgccagg gcagagtttt tcgcccgtat ttatcgtacc agtccagaac
     8941 ctgggctgaa aattgcgacg cttgcatggt caccgaattc actgttgttg ggggcaagat
     9001 tgcagcacag cgacggcagg gtgtaaaccg gaactttccg cagccacggc cttatcatgc
     9061 ttgcatccgg caactaactt tggataatgc ccgttttcag aacactttca caagcgacta
     9121 aatctttatg aaaaacgacg tcatttcacc ggaatttgat gaaaacggcc gcccactgcg
     9181 ccgtatccgt agttttgtgc gccgccaggg gcgactgacc aaaggccagg aacatgcgct
     9241 ggaaaactac tggccggtga tgggcgttga gttcagcgaa gatatgctgg atttccccgc
     9301 gctttttggc cgtgaagcgc cggtgacgct tgagattggt tttggcatgg gggcgtcgct
     9361 ggtggcaatg gctaaagatc gccccgagca ggacttcctc ggcattgaag tgcattcacc
     9421 gggcgttggt gcgtgcctgg cttctgcgca tgaagagggc ttaagcaacc tgcgcgtgat
     9481 gtgtcacgat gcggttgaag tgctgcataa aatgattcct gacaattcat tgcgcatggt
     9541 gcagctcttt ttccctgacc cgtggcacaa agcgcgccat aataaacgcc gtatcgttca
     9601 ggtgccgttt gccgaactgg taaaaagcaa actgcagctg gggggcgtat tccatatggc
     9661 gaccgactgg gaaccttatg cggaacatat gattgaagtg atgtcttcta ttgacggtta
     9721 taaaaacctg tcagagagca atgattacgt accgcgtccg gcatcacgtc cggtgacgaa
     9781 atttgaacaa cgtggtcatc gtcttggtca cggagtatgg gacttaatgt tcgagagggt
     9841 gaaataatgg caaagaaccg tagccgtcgt ctgcgtaaaa aaatgcacat cgacgaattc
     9901 caggaattag gattttcggt ggcatggcga ttcccggaag gtacatcgga agaacagatt
     9961 gataaaaccg ttgatgattt tattaacgag gttatcgaac cgaacaaact ggcctttgac
    10021 ggcagcggtt atctggcctg ggaaggtctg atctgcatgc aggaaatcgg caaatgcacc
    10081 gaagaacatc aggcgattgt gcgtaagtgg ctggaagagc gcaacctgga tgaggtacgc
    10141 accagcgaac ttttcgacgt ttggtgggac taagaaagca tacgggcgat gacaaatgca
    10201 aaactgcctg atgcgctacg cttatcatgc ctacaaagat gcacgatcga gtaggcggga
    10261 taaggcgttt acgccgcatc cggcatggaa aacgcgtact ttgttatcaa tctggggcca
    10321 gcaaatgctg gcctgatttg ttcttgaggg aagactatga tgcgcaaaat gctgctggcg
    10381 gcagcacttt cagtgacggc aatgaccgct cacgccgact accagtgcag cgtcacgccg
    10441 cgtgacgatg tgattgtcag cccgcaaacc gtgcaggtga agggcgaaaa cggcaatctg
    10501 gtgatcacgc cagacggcaa cgtgatgtat aacggtaagc aatattccct gaatgccgcc
    10561 cagcgcgagc aggcgaagga ttatcaggct gaactacgta gcacgctgcc gtggattgat
    10621 gaaggcgcga aaagccgcgt cgagaaagcc cgtattgctc tggataaaat tatcgttcag
    10681 gagatgggcg aaagcagcaa aatgcgcagc cgtctgacca aacttgatgc gcagctgaaa
    10741 gagcagatga accgcattat cgaaacgcgc agcgatggcc tgacgttcca ctataaagcc
    10801 attgatcagg ttcgcgctga aggccagcaa ttagtgaatc aggcaatggg cggaatttta
    10861 caggacagca ttaatgaaat gggcgcgaaa gcggtgctga aaagcggcgg taacccatta
    10921 cagaatgtgc tgggaagcct gggggggctg caatcctcaa tccaaaccga gtggaaaaag
    10981 caggaaaaag atttccagca gtttggcaaa gatgtttgta gccgcgttgt gactctggaa
    11041 gatagccgca aagccctggt cgggaattta aaataatcct ctattttaag acggcataat
    11101 acttttttat gccgtttaat tcttcgtttt gttacctgcc tctaactttg tagatctcca
    11161 aaatatattc acgttgtaaa ttgtttaacg tcaaatttcc catacagagc taagggataa
    11221 tgcgtagcgt tcacgtaact ggaggaatga aatggagttt ttcaaaaaga cggcacttgc
    11281 cgcactggtt atgggtttta gtggtgcagc attggcatta cccaatatca ccattttagc
    11341 aaccggcggg accattgccg gtggtggtga ctccgcaacc aaatctaact acacagcggg
    11401 taaagttggc gtagaaaatc tggttaatgc ggtgccgcaa ctgaaggaca ttgcgaacgt
    11461 taaaggcgag caggtagtga atatcggctc ccaggacatg aacgatgatg tctggctgac
    11521 actggcgaaa aaaattaaca ccgactgcga taaaaccgac ggcttcgtca ttactcacgg
    11581 taccgacacg atggaagaaa ccgcttactt cctcgacctg acggtgaaat gcgacaaacc
    11641 ggtggtgatg gtcggcgcaa tgcgtccgtc cacgtctatg agcgcagacg gtccattcaa
    11701 cctgtataac gcggtagtga ccgcagctga taaagcctcc gctaatcgtg gcgtgctggt
    11761 ggtgatgaac gacaccgtac tggacggtcg cgatgtcacc aaaaccaaca ccaccgacgt
    11821 agcgaccttc aagtctgtta actacggtcc gctgggatac attcacaacg gtaagattga
    11881 ctaccaacgt accccggcac gtaagcacac cagcgacacg ccgttcgatg tctctaagct
    11941 gaatgaactg ccgaaagtcg gcattgttta taactacgct aacgcatccg atcttccggc
    12001 taaagccctg gtagatgcgg gctatgatgg catcgtgagc gctggtgtgg gtaacggcaa
    12061 cctgtataaa tccgtgtttg acacgctggc gaccgccgcg aaaaacggta ctgcagtcgt
    12121 gcgttcttcc cgcgtaccga cgggcgctac cactcaggat gccgaagtgg atgatgcgaa
    12181 atacggtttt attgcctctg gtacgctgaa cccgcaaaaa gcgcgcgtcc tgctgcaact
    12241 ggctctgacg caaaccaaag atccgcagca gatccagcag atcttcaatc agtactaatc
    12301 atttcgcccc ggtatcgcgc cggggctttt tcacttcaga cttacgtcca ttgccaattt
    12361 taattgccct aatgataatc accagaataa attattccgc gcgagggttt tcgggtgaaa
    12421 aagcaatgga ttgttggcac ggcgctgctt atgttgatga ctggtaatgt ctgggcagat
    12481 ggcgaaccgc caactgaaaa tatcttaaaa gatcaattca aaaagcagta tcacggcatt
    12541 ctcaagcttg atgccatcac cttaaaaaat cttgatgcta agggtaatca ggccacctgg
    12601 tcagcggaag gcgatgtctc ttccagtgac gatctctata cctgggtcgg tcagttggca
    12661 gattacgaac tgctcgaaca gacctggacg aaagataaac cggtaaaatt ctcggcgatg
    12721 ttaaccagta aaggaacgcc cgcgtctggc tggacggtga acttttactc ttttcaggcg
    12781 gcagctagcg atcgtggtcg ggtggtcgac gatatcaaaa cgaataataa atatctgatc
    12841 gtgaatagcg aagatttcaa ttatcgcttt agtcagcttg agactgcgtt gaatatccag
    12901 aagaactcga ttcctgcgct ggaaaaggaa gtgaaagcgc ttgataagca aatggtggcg
    12961 gcgcagaaag cagcggatgc atattgggga aaagatgcaa atggtaagca aatgacccgc
    13021 gaagatgcct ttaaaaaaat tcaccaacag cgtgatgagt ttaataaaca gaacgatagc
    13081 gaggcgttcg cggttaaata tgacaaagag gtttatcaac cggcgattgc ggcatgtcat
    13141 aaacagagtg aagagtgtta tgaagtgccg attcagcaga aacgagattt cgatatcaac
    13201 gaacaacggc gacagacttt tctgcaatca caaaaactta gccgtaaatt gcaggatgac
    13261 tggataacgc tcgaaaaagg gcaatatccg ttaacgatga aagtttcaga aataaacagt
    13321 aaaaaagtcg cgattttgat gaaaattgac gatatcaacc aggctaatga acgctggaaa
    13381 aaagataccg aacaactgcg gcgcaatggg gtgattaagt aagttttgat atagcgcacg
    13441 atttgtagca ggatgcggcg ttgaccccgc atccggcaat caacgtctga tgcgacgcta
    13501 gcgcgtctta tcagacctac aacagctgcc acatgtcggc tgaggtgctc acgccgcatc
    13561 cggcaatcaa cgtctgatgc gacgcttgcg cgtcttatca gacctacaac cgttgccaca
    13621 tgtaggctgg ataaggcgtt cacgccgcat ccggcaatac aagtttactc agccagaaaa
    13681 agctccagca gcgaatttaa aaacagcttc ccatgttccg taatctgcca gtaatccgca
    13741 cattcggtga gataaccctg ggcaatcgcc tcgtctaact gtgggcgaat cacatcttcg
    13801 caaagcccgg tatacgcact aaactccacg cgcggcgcag gttccagcaa ccggaagcga
    13861 ttcataaaga actcaaacgg cttatctgcg gcttcgacat cacgctggct ttccagatac
    13921 cttccttgca taaaaccacg cggatgacgc gttttggtgg tacgcagaat gcgcccatcc
    13981 gggaaggtca ctttgccgtg cgcgccgcag ccaataccga tgtagtcacc aaagcgccag
    14041 tagttgagat tgtgctggca ctgataaccg ggtttggcgt aagcggaagt ttcatattgc
    14101 tgataacccg ctgcggttaa taactgatgc ccctgttcga aaatatccca cagcgcgtcg
    14161 tcgtccggca gtaccggcgg gcgtgaacca aacagcgtat taggttcgat ggtcagttga
    14221 taccaggaaa gatgcggcgg attcagttca atagcctggc gcagatcgcc aagcgcctct
    14281 tccagcgatt gatccggtag cccatgcatc aaatcaaggt taaagctacg taaccctaaa
    14341 ccgctcgcca gctttgccgc tcgtttcgct tcttgcgggc catgaatacg tcccagccgt
    14401 ttcagctttt cttcgctaaa actctgtacc ccaatagaga tacgatttac accggcacgc
    14461 tgataatcaa caaagcgatc ggcttctacc gtaccggggt tcgcttccat ggtaatttct
    14521 gcatccgctg ccagcggcaa acgcgcacgc acgccgtcca gcagcgtttg catcgccgag
    14581 ccggaaagca ggctcggcgt accaccgcca ataaagattg tctttacttc acggccctga
    14641 gcgtaagcca catcgttgtc cagatcgtta agcagatgct gaacgtaatc gtcgtgcggc
    14701 acttctcctt tcaacgcgtg agagttgaaa tcgcagtacg ggcatttctg cacgcaccac
    14761 gggatgtgaa tgtagagact cagcggcggt aatttaacca ttacgtaaag cgtccagcag
    14821 cagtttcaat gcctgaccac ggtgggaaat ggcgctcttt tcttcgcggg tcagttcggc
    14881 agcggttttc ccttcggaag gtacgaagaa gattggatca taaccaaagc caccagtgcc
    14941 cgccggttcg cgagtaatca cacccggcca gctgccatgg cacaccagcg gggtcggatc
    15001 ttctgcgtga cgcagataca ccagcacgca gtggaaacgc gcctgacgtt ggtcgtccgg
    15061 tacgtctttc agtgtttcca gcagtttttg cagattcttt tgatcggtcg cgtcttcacc
    15121 ggaataacat gcggagtaaa tacccggcgc gccgccaagc acatctaccg ccaggccaga
    15181 gtcgtcggca attgccggta aaccggtcac ttttgccgcg tggcgcgctt tcagaatcgc
    15241 gttttcgata aaggtcaggc cggtttcttc agcggaatca acgccgagtt ctgtttgggc
    15301 cacgatatca agaccgaagt cgctaagcag cgacgccagc tcacgcactt taccggcatt
    15361 gccggttgcg aggacaactt tttgcatagg atacctaatt aattaacgcc gcgacttctg
    15421 gcgggatttg ttgcggatta atgattttaa tttgtttgtg gcggccaagt tcgccttttt
    15481 caatcaccac ctggcttttg gcaacccgga attgcttacc gagaaacttc accagatggc
    15541 tgttggcctg accgtcaacc ggcggcgcgg taatggcgac ttttacctcg tcgccatgta
    15601 aaccgacaat agaatcacgg ctggctttcg gctgaatata gagccgtaaa accagaccgt
    15661 catcgttaac tgttacggca ctcataacgc catccacagc cccggcagca gcatatttcc
    15721 ggtcgcctgt aatacttctg cgacacccat attgatgaca tacagcagca gaacgaggat
    15781 catcggcgag aaatcaatcc cccccattgc cggtagcaag cggcgaatcg ggcgcagcag
    15841 cggatcggcc agctgaatca gcacgtattc aatcgggcta cggccctggc ttacccagct
    15901 cataatcgcc atcaccagca ggacccagaa aatcagcagg ccgatggttt tcagcagaat
    15961 cagtaaaccg gcaatccaga tgattggcag gaaggtcacc actttaaaca gcacgatggc
    16021 tttgataaaa ctgagaatat aggcaaccag cagcgaggcg ctatcaatgg gtcccattgc
    16081 cggaataacg cggcgcagtg gcccgataat tggctgcgtt accttcacta caaactgtga
    16141 gaaggggttg taaaaatcac aatgagccca ctgcatccag atgcgtaata acagcaccat
    16201 ggtatacagc tcaatgaccg ttgaaagcag gaaagtcaac gtattcatgg cgttccttta
    16261 attccttatt ttttagagta atcacgcgca ccaaaaattg cagtgccgat acgaaccatc
    16321 gtgctacctg ccgcaatggc ggcttccata tcgtccgaca ttcccagaga gagcgtgtcg
    16381 atatgcgggt agcgcgtttt cagtccggca aatgctacag ccatttggcg tgcaacttca
    16441 aactgcctta catattctga ctcaggcgca gggattgcca tcagcccgcg cagacgtaaa
    16501 cgcggtagtt cagcgaccgc agctgccagc tcgtccagtt ctgccagttg aatcccggac
    16561 ttactgtttt catcactaat attaatttga atcagtacgt taagaggggg aagttctgcc
    16621 gggcgctgat cgttgaggcg ggtagcaatg cgcaaacggt cgatggtatg acaccagtcg
    16681 aaatgctctg ccaccaggcg gcttttatta gactgcaacg ggccaataaa atgccattct
    16741 aatcctgtta cgcccagttc ctgaaagtgg cgaattttat ctaccccttc ctgaacgtag
    16801 ttttcaccaa attgacgctg cccggcatca atggcttctg cgatggcgct cgcaggtttt
    16861 gttttactga ctgcaagcag cgtaatttct tctggagaac ggccgcaacg cgttgcagcc
    16921 gctgagattt tgtcccggac ctgtgccagg ttatgcgcaa tatcgttcat tttccgagga
    16981 tcttagtatg aatatggaag aaattgtggc ccttagtgta aagcataacg tctcggatct
    17041 acacctgtgc agcgcctggc ccgcacgatg gcgcattcgc gggctaatgg aagctgcgcc
    17101 gtttgatgcg ccggacgtcg aagagctact gcgggagtgg ctggatgacg atcagcgggc
    17161 aatattgctg gagaatggtc agctggattt tgccgtgtcg ctgacggaaa accagcgatt
    17221 gcgtggcagc gcattcgcgc aacggcatgg catttcgctg gcgttacggc tgttaccttc
    17281 gcactgcccg cagctcgaac agcttggcac accaccgata ttgccggagt tactcaagag
    17341 cgagaatggc ttgattctgg tgacgggggc gacggggagt ggcaaatcta ccacgctggc
    17401 ggcgatggtt ggttatctca atcaacatgc tgatgcgcat attctgacgc tggaagatcc
    17461 tgtggaatat ctctatgcca gccagcgatg tttgatccag cagcgggaaa tcggtttgca
    17521 ttgtatgacg ttcgcatcgg gattgcgggc cgcattgcgg gaagatcctg atgtgatttt
    17581 gctcggagag ctgcgtgaca gcgagacaat ccgtctggcg ctgacggcgg cagaaaccgg
    17641 gcatctggtg ctggcaacct tacatacacg tggtgccgcg caggcagttg agcgacttgt
    17701 ggattcattt ccggcgcagg aaaaagaccc cgtgcgtaat caactggcag gtagtttacg
    17761 ggcagtgctg tcacaaaagc tggaagtgga taaacaggaa ggacgcgtgg cgctatttga
    17821 attgctgatt aacacacctg cggtggggaa tttgattcgc gaagggaaaa cccaccagtt
    17881 accgcatgtt attcaaactg ggcaacaggt ggggatgata acgtttcagc agagttatca
    17941 gcagcgggtg ggggaagggc gtttgtgacg ttgccatgcc cgatgcgcaa gcttatcggg
    18001 cctacaaagg caataaacgt ctgatgcgac gcttgcgcgt cttatcagac ctacaaccgc
    18061 tgccacatgt aggccggata aggcgttcac gccgcatccg gcaataaggc gtaaggcgcg
    18121 atgatattat ttacactcca gcaggcgatt aaatctcctt aatatccctg ctcgaaatag
    18181 ctttcgagaa taataaccgc agaggcagag tcgattttgc ctttgttgag cgcccgatag
    18241 ccgccctgtt caaacagacc ggaacgtgcc tccacggtgc taagacgctc gtcatggagc
    18301 tttacttcaa caccgaaacg accatgaata cggttagcaa atttacgcgc tctggcggtt
    18361 aacggctgtt cggtgccgtc catattcagc ggtaaaccga cgatgatttc gtctggctgc
    18421 cactctttca gtaaacgttc gatgaggttc cagtccggcg taccgtcctg tgctttaatt
    18481 gcaggcaaag ggcgagcggt gccggtaatg cgttggccga ccgctacgcc aatgcttttg
    18541 gtgccgaagt cgaaggcgag taaggttcca ctcatcaggc gtgtcctgcc acaccaggca
    18601 tggtgagaat atccacacca atcagttttg ccgcctcgcg ccagcggtcg gcaattggcg
    18661 ttttgaacag aatattcaga tctgccgggg cggttagcca cgcgttatcg agaatttctt
    18721 gttccagttg acctttttcc caggaggcat agcccagagc caccaatacg tcagacggtt
    18781 gtttatcggt gccgagcgtt tccagcacat cgcgggaagt ggtcatcact gtgttgtctg
    18841 aaatgcgaat gctggaagca aaattggagg gcggagtatg caaaataaac ccgcgatctt
    18901 cagccagtgg gccgccgagc ataaccggtt tatccagacg gattgattca tcacgcggct
    18961 ccggcgtgat cttcagcttt tccagaatcc cttcaatttt gagattttcc agcggcttgt
    19021 tgacgatgat ccccattgca ccattggtat tatgttcgca aatgtagacc acggaacgac
    19081 ggaaaatcgg atcctggaga gcaggcatgg caataagaaa gtgatgctgt aaattcattg
    19141 tcagaggttc tgtttcctgg ttcaaaaaag caacagcgcc cagtatgcgt ggaaagaagg
    19201 gcgctgtcac tcagagtctc aacgagatcc ttctcgctaa ggtgggttac tactgctgta
    19261 aacgtgcttc gatggcatcc attaacattc cggtgatcga caccggaaac tctgcttcaa
    19321 tctcacgaat acaggttggg ctggtgacgt taatttcagt cagacggtcg ccgatgatat
    19381 ccagaccaac aaaaatcagc cctttttctt tcagcgtcgg cccgatctga cgggcgattt
    19441 tccagtcact ttccgtcagc ggacgaggtt caccgcgacc accggcagcc agattgccac
    19501 gggtttcgcc cccctgcgga atacgcgcca ggcagtacgg tactggttcg ccgtccacca
    19561 ccagcacgcg tttgtcgcca tctttaatgg ctggcagata attttgtgcc atgcagtagc
    19621 gagtgccgtg ttcggtcaag gtttcggcaa tgacgccgag gtttggatcg ccttctttca
    19681 cgcggaaaat cgacgcgccg cccataccgt ccagcggctt aagaatgatg tcgctgtgtt
    19741 tctcccagaa cgcttttagc tgcgctttat tgcgcgtaac cagcgtttct ggcgttaagt
    19801 cagagaacca ggcggtaaac agtttctcgt tacagtcgcg caggctctgc ggcttgttaa
    19861 cgatcagcgt ccctttctct tcggcacgtt ccagaatata ggtcgcgtag ataaactcgg
    19921 tatcaaacgg cgggtcttta cgcatcagga tcacatcgag atcggccagc ggcagatcct
    19981 gttcaccgac gaatgaaaac cactcttcgt agttctgttt cacgttcagc gtgcgggtat
    20041 gggcgcgggc ttcgccattg atcagataca gatcgcccat ctccatatag tgaagttcgt
    20101 aaccacgacg ctgtgcttcc agcaacatag cgaaactgga atctttcttg atgttgatgt
    20161 ttgcgatggg gtccatcacg atgccgagct tgatcattat tcttctccgt tagcccaaat
    20221 cgccaaatcg tacttgtagc gcggtaatgg cggtgagcgc agttgtctct gtacgcaaaa
    20281 cgcgaggtcc caacaggata tcagtaaatt gatagcgggc agtcatggca atttcatctg
    20341 ccgataaacc gccttccggg ccaatcagca ggcggacgcg ttcaaccggt aaaggcaacg
    20401 tattgatgct gttactggcg cgcgggtgaa gattcagttt cagtccttca tcctgctctg
    20461 cacaccaggc ttccagatcc atcgctggac ggatttccgg cacccggtta cgaccacact
    20521 gctcacaggc agcaattgca atcttctgcc actgctggag cttcttgttc agacgttcac
    20581 tatccagttt aacgccgcag cgctcagaaa aaagtggcgt aatgaggctt acaccgagtt
    20641 cgatcgattt ctggatcgta aattccattt tttcaccacg cgacatcacc tgaccgaggt
    20701 gaatatgcag cggagattcg cgatcgtcga tctggccttc cagcaccttc acttccacgc
    20761 tttttttgct ggcgctggta atttcggcgt caaagacctg gttgctgccg tcaaacaatt
    20821 gcaacgcctg tcccggcccc atacgcagta cgcgcccaat atggttggcg gcatcttcgc
    20881 aaagcgcgat gtgagaatgg ctggtcagtg gttcaggatg ataaatgcgg gggatacgca
    20941 tagttaaaaa tccgcgtcgt ctccccacgc ggttgtacgc gtggggtagg ggttaacaaa
    21001 aagaatcccg ctagtgtagg ttagctcttt cgcgcctggc aagcgcgttg cacatacggg
    21061 ttatgattgc cctgtacctt cgcgatgcgt tcatcgcgct cgcactccca gtcggtaacc
    21121 ggatacatct tgtcccatgc gttgaacagc tgcgtttgct ggcgagagag tgtcaggttg
    21181 taatggtcac gcatatagaa gtaggtgcgc gcaatggcac cgcgtgcacg cgctggtggt
    21241 tcggcagctt tttctttgaa atcgaccttc atggcgcatt gaccgtactg gccttcaccg
    21301 ccattccact ggctgtacat aaagttgccg cgatcgccat tcacctcgcc gactgacggt
    21361 tgcaggttat gcatatcgct ttccatcttg cgatagaccg gatctttggc gcaattttta
    21421 cgtccaccgt cctgccagca ctggcgctgg tgaccgaatt gccaggcggg aacgacgtgt
    21481 tcccactcta cgcggctggc gcggttttca tttttgcgca cctgatagcc gcacgattgc
    21541 agatcgacaa cgcctttttt gccctgccag ttaattttac atccgcaata aaacgtaccg
    21601 ggcgcgtcag cgtggacttt taccgccgcg gctttcgcct gagaaaaact attgatacct
    21661 tcggccaacg ccgggccgga aaatgctgcg ctcagtacca ccgcagcaat agacaaataa
    21721 cggtacatca cttactccgt gttaaaacga gccagcaacg tagcgaaagt tgttttggta
    21781 tgcaatcagc cagatcagga aagttcctga tagttcagat ggttattttg cgaccagttg
    21841 ttcgccgcag tgaacacagc gatagacggc ttcgccacga actacgcgat tatgacggcg
    21901 tacggtaagc tgatgttcct gacacttaca gctgtagggg aaggtgttgc ggcgcacgga
    21961 ttgcagttcg aactgatgcg tacggcgggc tggaacgccc agcacacttt ccatcatcca
    22021 cttccactct ttgccatgtg gtgctacgcg gccaaaatgt ttccatacca gcaaatgtgc
    22081 cagttcgtgc ggtaccactt cttcaataaa ggcttcactg ttttccaaca gcaaaacggg
    22141 attaaggcga atttcatagc tttccagcca ggctgttccg gcagaggttc cgcgctgggt
    22201 gtaagagagt tttggctccg ggtagttacg ccccaatttc aggttggcct gggcgagttt
    22261 ttcccgcagg cgacgcataa cggcctgctg gatggcgata gggagacggg atgttttcat
    22321 agcggcagag gatagagcga agaaaagatg actgcaagag gtggcttcct ccacgacggg
    22381 aggaagcctg gggagattaa tcgtgagcgc cgatttcgcg cagtttacga cctttcatca
    22441 gattacgttc aatatgttcc agcgaaacgt gtttggtttc tggcaccagc cacagtgtca
    22501 gcaggataaa caggacgttc agacccgcgt aaacccagaa ggtattagcg ttgcccagcg
    22561 tgttgagcat ggtcaggaac gttgcgccaa cgatcatgtt ggcaatccag ttggtggcgg
    22621 tggagcaggt gatgccaaaa tcgcggcctt tcagcggctg aatttcggag cacagtaccc
    22681 aaatcagcgg accggcactc atggcaaaac cgacaataaa catcagcagc atggcgatgg
    22741 cgaaatactg cgccgacgga gagtggatac cgatatgcat cattgtaccg agtacgccca
    22801 taccagcagc catcaccagg aagcccagcg ttagcgttgg tttacgtccc cagcggtcaa
    22861 caaggccgat tgcgataaag gtggcaagta cgttggtcag gccgacaatc actgtccccc
    22921 acatttgctc ggtggtgttg gtataacccg ccagttcgaa gattttcggc gcgtaataca
    22981 tgatgacgtt catcccggtg aattgctgca ttacctgtaa cagtacgcca aggaacaccg
    23041 cgcggcggaa gttgctattc tctttaaaca gcgcccagcc actctgttta acctgcaaac
    23101 tttcacggat ttcatccagt tcgcgtttcg cttccgcgct ggtgtcacgc aggcgtagca
    23161 gcacgcgttc ggcatcaaca aaacggcgtt tggcggcaaa ccaacgtggg ctgtctggca
    23221 ggaagaagac accaatcagc agcaaaattg ccgggatgat aatcacaccc agcatccagc
    23281 gccatgcacc ggtgtagctg aaggcggtat cagaaagata agcaccgagg atcccgattg
    23341 tgatcattaa ctgatacatc gagatcatac tgccgcgaat tttttccggc gcaatttcag
    23401 agaggtacag tggtgcggta taagaggcca cacctaccgc cagccccagc agaacgcggg
    23461 aaagaatcag tacttcaacg tttggcgcag ccgcagagaa cagcgaaccg gcaacaaaca
    23521 aaattgcgcc gatcatcagg ctctttttgc gcccgagttt aaaggagagc cagccgctgc
    23581 ccaccgcacc gactgccgca ccgaacatca tggagcttac gacccattct tgcgtgtgcg
    23641 aagtaatctg gaattcatct gcaataaacg gcagtgcgcc agcaattaca ccgatatcca
    23701 ggccaaagag taatcccgcc agagcggcaa ggaagcagac gaaaaacgtc attgccttgt
    23761 ttgactgccc ctgtttttta gcgtcaggca tgatgccctc caatatggtt attttttatt
    23821 gtgaattaag ataggtgagt acgacgtaaa a
//
LOCUS       DBFIFI010000056        23851 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.056, whole genome shotgun sequence.
ACCESSION   DBFIFI010000056 DBFIFI010000000
VERSION     DBFIFI010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23851
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.056"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            190..1854
                     /gene="mhpA"
                     /locus_tag="N7B39_002109"
     CDS             190..1854
                     /gene="mhpA"
                     /locus_tag="N7B39_002109"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HGU2422092.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVDNVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGVSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     LREIVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALSTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGKTLNKLASVMTLTRPDADVSVEKVA"
     gene            1856..2800
                     /gene="mhpB"
                     /locus_tag="N7B39_002110"
     CDS             1856..2800
                     /gene="mhpB"
                     /locus_tag="N7B39_002110"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HGU2422093.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            2818..3684
                     /gene="mhpC"
                     /locus_tag="N7B39_002111"
     CDS             2818..3684
                     /gene="mhpC"
                     /locus_tag="N7B39_002111"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308431.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HGU2422094.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARA"
     gene            3694..4503
                     /gene="mhpD"
                     /locus_tag="N7B39_002112"
     CDS             3694..4503
                     /gene="mhpD"
                     /locus_tag="N7B39_002112"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HGU2422095.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQYDVAQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAAAFSSAAPKGSLS"
     gene            4500..5450
                     /gene="mhpF"
                     /locus_tag="N7B39_002113"
     CDS             4500..5450
                     /gene="mhpF"
                     /locus_tag="N7B39_002113"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HGU2422096.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHDQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEENVDQLNVNMVTCGGQATIPMVAAVSRVVRVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            5447..6460
                     /gene="mhpE"
                     /locus_tag="N7B39_002114"
     CDS             5447..6460
                     /gene="mhpE"
                     /locus_tag="N7B39_002114"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HGU2422097.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHRYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAVDKLGWQHGADLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            6636..7847
                     /gene="mhpT"
                     /locus_tag="N7B39_002115"
     CDS             6636..7847
                     /gene="mhpT"
                     /locus_tag="N7B39_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HGU2422098.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRLRMQPCADA"
     gene            7949..8488
                     /gene="yaiL"
                     /locus_tag="N7B39_002116"
     CDS             7949..8488
                     /gene="yaiL"
                     /locus_tag="N7B39_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HGU2422099.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(8613..9446)
                     /gene="fghA"
                     /locus_tag="N7B39_002117"
     CDS             complement(8613..9446)
                     /gene="fghA"
                     /locus_tag="N7B39_002117"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HGU2422100.1"
                     /translation="MELIEKHASFGGWQNVYRHYSASLKCEMNVGVYLPPKAAGEKLP
                     VLYWLSGLTCNEQNFITKSGVQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMNHFPATAKKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKEAWLDYHPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTQNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(9539..10648)
                     /gene="frmA"
                     /locus_tag="N7B39_002118"
     CDS             complement(9539..10648)
                     /gene="frmA"
                     /locus_tag="N7B39_002118"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HGU2422101.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            complement(10683..10958)
                     /gene="frmR"
                     /locus_tag="N7B39_002119"
     CDS             complement(10683..10958)
                     /gene="frmR"
                     /locus_tag="N7B39_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HGU2422102.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            complement(11118..12164)
                     /gene="fbpC"
                     /locus_tag="N7B39_002120"
     CDS             complement(11118..12164)
                     /gene="fbpC"
                     /locus_tag="N7B39_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric ABC transporter ATP-binding protein"
                     /protein_id="HGU2422103.1"
                     /translation="MSQKNFVELRNVTKRFGSNTVIDNINLTIPQGQMVTLLGPSGCG
                     KTTILRLVAGLEKPSEGQIFIDGEDVTHRSIQQRDICMVFQSYALFPHMSLGENVGYG
                     LKMLGVSRSEVKQRVKEALAMVDLEGFEDRYVDQISGGQQQRVALARALILKPKVLLF
                     DEPLSNLDANLRRSMRDKIRELQKQFNITSLYVTHDQSEAFAVSDTVLVMNKGHIMQI
                     GSPQDLYRQPASRFMASFMGDANLFPANFSEEYVDIYGYRLPRAAHFPVQGSGTVGVR
                     PEAITLSNHGEESQRCVIRHVAYMGPQYEVTVEWHGQEILLQVNATRLQPDIGEHYYL
                     EIHPYGMFVLADAA"
     gene            complement(12176..14254)
                     /locus_tag="N7B39_002121"
     CDS             complement(12176..14254)
                     /locus_tag="N7B39_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU2422104.1"
                     /translation="MSQTLALHPVKKRDAIFLWVLLGWLAFVLLPSWSLDYGLLESTS
                     DEILGAYGWSHFNISWLWYLLPSLLLVRPFRQATREQRSRHYFDAGWALLCMAFIVIS
                     ATVAGRGLGYAAIVLFVELGAIMTLALTRLEWLGGDRFVIGSLIAIVALIGIFIVWPS
                     IAIFIPMFTNDAGELAPLAFMTVLSQAHIVQVIVNSIVLSIAVGIGCAFFGLVLAIYT
                     TRIAKRSAIIGRIFSILPIVTPPFVVGLGVTLMMGRSGYVTEFMVEWFGLTNTNWLYG
                     FTGIWLAQVLAFTPMAFMILDGAIKTIHPSLEEASYTLRASRWQTFNGVFVPLLKPAL
                     ANAFLIVVVQSLADFSNPLVLGGNFDVLATQIYFYITGSQLDYQAASTLGAFLLLFSL
                     LVFCIQYMWIGKRSYVTVSGKSYRGDVQPLPVTLVWSVVAILAIWIAFNALLYGSIFY
                     GSFTVNWGVDYTLTLDNFIKLFGQGMSDGAWPSLLDTLLYAGIAAPITAIFGLLIAWV
                     VVRQQFKGKKTIEFTTMLCFAVPGTVAGVSYILAFNSAPVYLTGTAAIVIISMVMRNV
                     PVGIRAGIAGLGQIDKSLDEASLSLRAGSLRTITHILLPLLRPAILSALIYSFVRAIT
                     TVSAIVFLVTPDTRVATAYILNRVEDGEYGVAIAYGSILIVVMLAIIFLFDWLIGEAR
                     ISRSKAKNQA"
     gene            complement(14323..15354)
                     /locus_tag="N7B39_002122"
     CDS             complement(14323..15354)
                     /locus_tag="N7B39_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGU2422105.1"
                     /translation="MKMTFTSTLIASAVALATLTGAAQAKGRLVVYCSATNEMCEAET
                     KAFGEKYDVKTSFIRNGSGSTLAKVDAEKKNPQADVWYGGTLDPQSQAGEMGLLQAYK
                     SPNLEQIMEKFRDPAKVKGNLSSAVYVGILGFGVNTDRLKEKNLPVPKCWKDLTKPEY
                     KGEIQIADPQSSGTAYTALATFAQLWGEDQAFDYLKQLNANVSQYTKSGIAPARNAAR
                     GETAIGIGFLHDYSLEKEQGAPLELISPCEGTGYEIGGVSILKGARNLDNAKLFVDWV
                     LSKEAQELAWKKGKSYQILTNTTAETSPNSLKLDDLKLINYDMDKYGSTDVRKALINK
                     WVSEVKMGK"
     gene            complement(15351..16667)
                     /gene="uhpC"
                     /locus_tag="N7B39_002123"
     CDS             complement(15351..16667)
                     /gene="uhpC"
                     /locus_tag="N7B39_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HGU2422106.1"
                     /translation="METTMHARSAREINQCYRTLRPRLLMCMIIGYAAFYLTRKSVNY
                     VLPALQTDLGLDKGDIGLLGSLFYLSYGLSKFTAGLWHDSHGQRGFMGVGLFATGLLN
                     VVFAFGESLTLLLVVWTLNGFFQGWGWPPCARLLTHWYSRNERGFWWGCWNMSINIGG
                     AIIPLISAFAAHWWGWQAAMLTPGIISMALGIWLTLQLKGTPQEEGLPTVGHWRHDPL
                     ELRQEQQSPPMGLWQMLRTTMLQNPLIWLLGVSYVLVYVIRIALNDWGNIWLTESHGV
                     NLLSANATVMLFEVGGLLGALFAGWGSDLLFSGQRAPMILLFTLGLMVSVAALWLAPV
                     HHYALLAVCFFTVGFFVFGPQMLIGLAAVECGHKAAAGSITGFLGLFAYLGAALAGWP
                     LSLVIERYGWPGMFSLLSVAAVLMGLLLMPLLMAGITTTHARRIKQ"
     gene            complement(16740..18281)
                     /locus_tag="N7B39_002124"
     CDS             complement(16740..18281)
                     /locus_tag="N7B39_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HGU2422107.1"
                     /translation="MSRNLRHWVILLFIVLAWGTGWLMLWTLSFYLTNNGQQAVLFLP
                     QGVYLALVILLSRRYWPALVLPPLLMMFWLHSEQLLNGYLMLATPVISLFPALLAQNF
                     WHRFPLYWQRLTLLLATVTAASLLNTALLSPFMSGPIMLPGLTSFTGGVLLTPFVYLI
                     FEFLRQQHRYQLLGLDTHNPPLRTSLIIWCSLFFIIGIGTQMVLSPEIERLLLIVVFL
                     PNVVMAWKFGWQGGVLSGLLGSMMITIARQIGVGFSNLVELEIFLATQALLGIGLGIA
                     ISRQQHLALNLHHYRQRLEAELAARRALAEKLIHTEEDTRKNLARELHDEIGQNITAI
                     QIQSQLVKRARDPAQIQSAASQINELARRIHLSTRQLLRQLRPPALDELTFREALLHL
                     INEFAFSERGIHCQFAYQLNSTPENETVRFTLYRLLQELLNNICKHAEASEVTIILRQ
                     QGEVLHLEVSDNGVGIASGKMAGFGIQGMRERVSALGGDLTLEKQHGTRVIVNLPTFL
                     QQIAG"
     gene            complement(18281..18910)
                     /locus_tag="N7B39_002125"
     CDS             complement(18281..18910)
                     /locus_tag="N7B39_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HGU2422108.1"
                     /translation="MIRVVLVDDHVVVRSGFAQLLSLEDDLEVIGQYSSAAQAWSALI
                     RDDVNVAVIDIAMPDENGLSLLKRLRAQKPQFRAIILSIYDAPIFVQSALDAGASGYL
                     TKRCGPEELVQAVRSVGLGGHYLCADAIRALRGGGQPAQALEILTPREREVFELLVKG
                     DSVKEIAFKLELSHKTVHVHRANVLGKLNCHSTIELVHFALDHHLLAGH"
     gene            19213..20175
                     /gene="tauA"
                     /locus_tag="N7B39_002126"
     CDS             19213..20175
                     /gene="tauA"
                     /locus_tag="N7B39_002126"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU2422109.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDVWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            20188..20955
                     /gene="tauB"
                     /locus_tag="N7B39_002127"
     CDS             20188..20955
                     /gene="tauB"
                     /locus_tag="N7B39_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HGU2422110.1"
                     /translation="MLQISHLYADYGGKPVLEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     IQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSPGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            20952..21779
                     /gene="tauC"
                     /locus_tag="N7B39_002128"
     CDS             20952..21779
                     /gene="tauC"
                     /locus_tag="N7B39_002128"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HGU2422111.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVLIGIPVG
                     IAMGLSPTVRGILDPIIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            21776..22627
                     /gene="tauD"
                     /locus_tag="N7B39_002129"
     CDS             21776..22627
                     /gene="tauD"
                     /locus_tag="N7B39_002129"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HGU2422112.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(22734..23708)
                     /gene="hemB"
                     /locus_tag="N7B39_002130"
     CDS             complement(22734..23708)
                     /gene="hemB"
                     /locus_tag="N7B39_002130"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HGU2422113.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
BASE COUNT         5528 a         6268 c         6693 g         5362 t
ORIGIN      
        1 ttaattgaca tttctatagt taaaacaacg ttgtgcacct ggtgcacatt cgggcatgtt
       61 ttgattgtag ccgaaaacac cctttctata ctgagcgcac aataaaaaat catttacata
      121 tttttaacaa aataagttgc gctgtactgt gcgcgcaacg gcattttgtc cgagtcgtga
      181 ggtactgaaa tggcaataca acaccctgac atccagcctg ctgttaacca tagcgttcag
      241 gtggcgatcg ctggtgccgg tccggtcggg ctgatgatgg cgaactatct cggccagatg
      301 ggcattgacg tgctggtggt ggagaaactc gataagttga tcgactaccc gcgtgcgatt
      361 ggtattgatg acgaggcgct gcgcaccatg cagtcggtcg gcctggtcga taatgttctg
      421 ccgcatacta cgccgtggca cgcgatgcgt tttctcaccc cgaagggccg ctgttttgct
      481 gatattcagc caatgaccga tgaatttggc tggccgcgcc gtaacgcctt tattcagccg
      541 caggtcgatg cggtgatgct ggaaggggtg tcgcgttttc cgaatgtgcg ctgcttgttt
      601 tcccgcgagc tggaggcctt cagtcagcaa gatgacgaag tgaccttgca cctgaaaacg
      661 gcagaagggc tgcgggaaat agtcaaagcc cagtggctgg tggcctgtga tggtggggca
      721 agttttgtcc gtcgcaccct gaatgtgccg tttgaaggta aaactgcgcc aaatcagtgg
      781 attgtggtag atatcgccaa cgatccgtta agtacgccgc atatctattt gtgttgcgat
      841 ccggtgcgcc cgtatgtttc tgccgcgcta cctcatgcgg tacgtcgctt tgaatttatg
      901 gtgatgccgg gagaaaccga agaacagctg cgtgagccgc aaaatatgcg caagctgtta
      961 agcaaagtgc tgcctaatcc ggacaatgtt gaattgattc gccagcgtgt ctacacccac
     1021 aacgcgcgac tggcgcaacg tttccgtatt gatcgcgtac tgctggcggg cgatgccgcg
     1081 cacatcatgc cggtatggca ggggcagggc tataacagtg gtatgcgcga cgcctttaac
     1141 ctcgcatgga aactggcgtt ggttatccag gggaaagccc gcgatgcgct gctcgatacc
     1201 tatcaacaag aacgtcgcga tcacgccaaa gcgatgattg acctgtccgt gacggcgggc
     1261 aacgtgctgg ctccgccgaa acgctggcag ggtacgttac gtgacggcgt ttcctggctg
     1321 ctgaattatc tgccgccagt aaaacgctac ttcctcgaaa tgcgcttcaa gccgatgccg
     1381 caatattacg gcggtgcgct ggtgcgtgag ggcgaagcga agcactctcc ggtcggcaag
     1441 atgtttattc agccgaaagt cacgctggaa aacggcgacg tgacgctgct cgataacgcg
     1501 atcggcgcga acttcgcggt aattggctgg ggatgcaatc cactgtgggg gatgagcgac
     1561 gagcaaatcc agcagtggcg cgcgttgagc acacgcttca ttcaggtggt gccggaagtg
     1621 caaattcata ccgcacagga taaccacgac ggcgtactac gcgtggggga tacgcaaggt
     1681 cgcctgcgta gctggttcgc gcaacacaat gcttcgctgg tggtgatgcg cccggatcgc
     1741 tttgttgccg ccaccgccat tccgcaaaca ctgggcaaga ccctgaataa actggcgtcg
     1801 gtgatgacgc tgacccgccc tgatgccgac gtttctgtcg aaaaggtagc ctgatatgca
     1861 cgcttatctt cattgtcttt cccactcgcc gctggtgggg tatgtcgacc cggcacaaga
     1921 ggtactcgat gaggtcaatg gcgtgattgc cagcgcccgc gagcgtattg cggctttctc
     1981 ccctgaactg gtggtgctgt ttgcgccaga tcactacaac ggctttttct atgacgtgat
     2041 gccaccgttc tgtttaggcg ttggagcgac ggcaattggt gatttcggca gtgcggcagg
     2101 agagctgccc gtgcctgtgg agctggcgga ggcctgtgcg catgccgtca tgaagagcgg
     2161 gatcgatctt gccgtttctt actgtatgca ggtggaccac gggttcgccc agccgctgga
     2221 gttcctgctc ggtgggctgg ataaggtgcc agttctgcct gtgttcatca acggtgtcgc
     2281 cacgccgctg cccggtttcc agcgtacccg catgttgggt gaagccattg gacgtttcac
     2341 cagcactctc aataaacgcg tgctgttcct aggttccggt gggctttccc atcagccgcc
     2401 ggtgcccgaa ctggcgaaag ccgatgccca tatgcgcgac cgcctgttgg ggagcgggaa
     2461 agatttaccc gccagtgagc gcgaattgcg tcagcaacgg gtgattagcg ccgctgagaa
     2521 gtttgttgag gatcagagaa ctctgcatcc gctcaacccg atttgggata accagttcat
     2581 gactttgctg gagcagggac gcatacagga actggatgcc gtcagtaacg aagagctttc
     2641 cgccattgcc ggaaagtcga cacatgaaat caaaacctgg gtcgccgctt ttgccgctat
     2701 ttctgcattt ggcaactggc gtagcgaagg gcgttattac cgcccaatcc cggagtggat
     2761 tgccggattt ggctcgttaa gcgccagaac agagaactga atatgcagga gaagatgatg
     2821 agttatcagc cacaaaccga agccgccacc agccgttttc tgaatgtaga agaagcgggt
     2881 aaaacgctgc gcatccattt taatgactgc ggacaaggcg acgaaaccgt tgtcctgctg
     2941 catggttccg gcccgggtgc tactggctgg gcgaacttca gccgcaatat cgatccgctg
     3001 gtagaggcgg gctatcgggt gatcctgctg gattgtccgg gttggggcaa gagcgattcg
     3061 atcgttaata gtggttcgcg atcggatctt aatgcacgaa tcctgaaaag cgtggtggat
     3121 caactggata tcgccaaaat ccacctgctg ggcaactcga tgggtggcca tagttctgtg
     3181 gcgttcaccc ttaactggcc ggagcgcgtc ggcaaactgg tgctgatggg cggcggtacg
     3241 ggcggcatga gtttgtttac gccgatgcca accgaaggta ttaagcgact gaatcagctt
     3301 tatcgtcagc cgactatcga aaacctgaag ctgatgatgg atatcttcgt ttttgatacc
     3361 agcgatttga ccgacgccct gtttgaagcg cgcctgaata atatgctgtc gcgccgcgat
     3421 cacctggaaa acttcgttaa gagcctggaa gctaatccga aacagttccc ggattttggc
     3481 ccacgtctgg cggaaatcaa agcgcaaacc ctgattgtct gggggcgcaa cgaccgcttt
     3541 gtgccgatgg atgcgggtct gcgtctgctg tccggcattg ccggttctga actgcatatc
     3601 ttccgcgact gtgggcattg ggcgcagtgg gaacatgccg acgctttcaa tcaactggtg
     3661 ctgaatttcc tcgcacgtgc ttaaggaacg gtcatgacga agcatactct tgagcaactg
     3721 gcggcggatt tacgccgcgc cgcagagcag ggcgaagcga ttgcgccgtt gcgcgatctg
     3781 attggtatcg ataacgctga agcggcctac gccattcagc acataaatgt gcaatatgac
     3841 gttgcgcagg ggcgtcgcgt ggtggggcgt aaagtgggcc tgacacatcc gaaagtgcag
     3901 caacaactgg gcgttgatca accggatttc gggacattat ttgccgacat gtgttatggc
     3961 gataacgaaa tcattccttt ttcccgtgtt ctgcaacccc gcattgaagc ggagatcgca
     4021 ctggtgttga accgcgattt acccgcaact gatatcacct tcgacgaatt gtataacgcc
     4081 attgaatggg tacttccggc gctggaggtg gtgggtagcc gcattcgcga ctggtcgatt
     4141 cagtttgtcg ataccgtggc agataacgct tcctgcgggg tgtatgtcat cggcggtccg
     4201 gcgcaacgtc cggcggggtt agacctgaaa aactgcgcca tgaagatgac gcgtaataac
     4261 gaagaggttt ccagcgggcg cggcagcgaa tgcctgggac atccgcttaa tgccgccgtc
     4321 tggctggcac gcaaaatggc cagtctgggt gaaccgctgc gcgccggaga tatcattctt
     4381 accggggcat taggtccgat ggtggcagtg aatgcgggcg atcgttttga agcccatatt
     4441 gaaggcatag gttcagttgc tgcggcattt tcaagcgcag ccccaaaagg aagtctgtca
     4501 tgagtaagcg taaagtcgcc attatcggtt ctggcaacat tggcaccgat ctgatgatta
     4561 aaattttgcg tcacgatcag catctggaga tggcggtgat ggtcggcatt gatcctcagt
     4621 ccgacggtct ggcgcgtgcc agacgtatgg gcgtcgccac cacccatgaa ggggtgatcg
     4681 ggctgatgaa catgcctgaa tttgctgata tcgacattgt atttgatgcg accagcgccg
     4741 gtgctcatgt gaaaaacgat gcggctttgc gcgaagcgaa accggatatt cgcttaattg
     4801 acctgacgcc tgctgccatc ggcccttact gcgtgccggt ggttaacctc gaggagaacg
     4861 tcgatcaact gaacgtcaac atggtcacct gcggcggcca ggccaccatt ccaatggtgg
     4921 cggcagtttc acgcgtggtg cgtgttcatt acgccgaaat tatcgcttct atcgccagta
     4981 aatctgccgg acctggcacg cgtgccaata tcgatgaatt tacggaaacc acttcgcgag
     5041 ccattgaagt ggtgggcggc gcggcaaaag ggaaggcgat tatcgtgctt aacccagcag
     5101 agccaccgtt gatgatgcgt gacacggtgt atgtattgag cgacgaagct tcacaagatg
     5161 atatcgaagc ctcaatcaat gaaatggctg aggcggtgca ggcttacgta ccgggatatc
     5221 gcctgaaaca gcgcgtgcag tttgaagtta tcccgcagga taaaccggtt aatttgccgg
     5281 gcgtagggca gttctccggg ctgaaaacgg cggtctggct ggaagtcgaa ggcgcagcgc
     5341 attatctgcc tgcctatgcg ggcaacctcg acattatgac ttccagtgcg ctggcgacag
     5401 cagaaaaaat ggcccagtca ctggcgcgca aggcaggaga agcggcatga acggtaaaaa
     5461 actttatatc tcggacgtca cgttgcgtga cggtatgcac gccattcgtc atcggtattc
     5521 gctggaaaac gttcgccaga ttgccaaagc actggacgat gcccgcgtgg attcgattga
     5581 agtggcccac ggcgacggtt tgcaggggtc cagctttaac tatggtttcg gcgcacatag
     5641 cgaccttgaa tggattgaag cagcggcgga tgtggtgaag cacgccaaaa tcgcgacgtt
     5701 gttgctgcca ggaatcggca ctattcacga tctgaaaaat gcctggcagg ctggcgcgcg
     5761 ggtggttcgt gtggctacgc actgtaccga agctgatgtt tccgcccagc atattcagta
     5821 tgcccgcgag ctcggaatgg acaccgttgg ttttctgatg atgagccata tgaccacgcc
     5881 ggagaatctc gcaaagcagg caaagctgat ggaaggctac ggtgcgacct gtatttatgt
     5941 ggtggattct ggcggtgcga tgaacatgag cgatatccgt gaccgtttcc gcgccctgaa
     6001 agcagtgctg aaaccagaaa cgcaaaccgg catgcacgct caccataacc tgagtcttgg
     6061 cgtggcgaac tctatcgcgg cggtggaaga gggctgcgac cgaatcgacg ccagcctcgc
     6121 gggaatggga gcgggcgcag gtaacgcgcc gctggaagta tttattgccg ccgtggataa
     6181 actgggttgg cagcatgggg ccgatctcta tgcgttaatg gatgctgctg acgacctggt
     6241 gcgtccgttg caggatcgac cggtacgagt cgatcgcgaa acgctggcgc tgggatacgc
     6301 tggtgtttac tccagcttcc tgcgtcactg tgaaacggcg gcggcgcgtt atggcttaag
     6361 cgcggtggat attctcgttg agctgggcaa acgccggatg gttggcggcc aggaggatat
     6421 gatcgtcgac gtggcgctgg atctgcgcaa caacaaataa tattgactgc cgagaacgtg
     6481 cattttgtat gcccatccca gcacgactgc cggatgcggc gtgaacgcct tatccggtct
     6541 acatttcgcc cgtaggcagt cattaaatag ttctgattac gcggctggca atgtatcagt
     6601 cgcgattcac ctgcacacgc aatgaggttc atcttatgtc gactcgtacc ccttcatcat
     6661 cttcatcccg cctgatgctg accatcgggc tttgtttttt ggtcgctctg atggaagggc
     6721 tggatcttca ggcggctggc attgcggcgg gtggcatcgc ccaggctttc gcactcgata
     6781 aaatgcaaat gggctggata ttcagcgccg gaatactcgg tttactaccc ggcgcgttgg
     6841 ttggcggaat gctggcggac cgttatggtc gcaagcgcat tttgattggc tcagttgcgc
     6901 tgtttggttt gttctcgctg gcaacggcga ttgcctggga tttcccctca ctggtctttg
     6961 cgcggctgat gaccggtgtc gggctggggg cggcgttgcc gaatcttatc gccctgacgt
     7021 ctgaagccgc gggtccacgt tttcgtggga cggcagtgag cctgatgtat tgcggtgttc
     7081 ccattggcgc ggcgctggcg gcgacactgg gtttcgcggg ggcaaactta gcatggcaaa
     7141 cggtgttttg ggtaggtggt gtggtgccgt tgattctggt gccgctatta atgcgctggc
     7201 tgccggagtc ggcggttttc gctggcgaaa aacaggctgc gccaccactg cgtgcgttat
     7261 ttgcgccaga aacggcaacc gcgacgctgc tgctgtggct gtgttatttc ttcactctgc
     7321 tggtggttta catgttgatc aactggctac cgctgctttt ggtggagcaa ggattccagc
     7381 catcgcaggc ggcaggggtg atgtttgcac tgcaaatggg tgcggcgagc gggacgttaa
     7441 tgttgggcgc attgatggat aagctgcgtc cagtaaccat gtcgctactg atttatagcg
     7501 gcatgttagc ttcgctgctg gcgcttggaa cggtgtcgtc atttaacggt atgttgctgg
     7561 cgggatttgt cgcggggttg tttgcgacag gtgggcaaag cgttttgtat gccctggcac
     7621 cgttgtttta cagttcgcag atccgcgcaa caggtgtggg aacagccgtg gcggtagggc
     7681 gtctgggggc tatgagcggt ccgttactgg ccgggaaaat gctggcatta ggcactggca
     7741 cggtcggcgt aatggccgct tctgcaccgg gtattcttgt tgctgggttg gcggtgttta
     7801 ttttgatgag tcggagatta agaatgcagc cgtgtgccga tgcctgacgc gccttattaa
     7861 gcaggggcga aagggacttg cccccaatac acccacggcg tttacaggta tactcgctaa
     7921 aaattattca gcgggtttgg aaacaaagat ggcaaaactt accttacaag agcagttgct
     7981 caaagcagga ttagtcacca gcaaaaaagc ggcgaaggtc gagagaacgg cgaaaaaatc
     8041 gcgcgttcag gcgcgtgaag ctcgggcggc ggtagaagaa aataaaaagg cacagcttga
     8101 gcgtgataaa cagcttagcg aacagcaaaa acaagcggcg ttggcgaaag aatataaagc
     8161 tcaggtgaag cagcttattg aaatgaaccg gatcaccatt gccaatggcg atattggttt
     8221 taacttcact gatggcaatc tgattaagaa gatttttgtc gataagctca cacaggcgca
     8281 gttgattaat ggtcgtctgg cgattgcccg cttgttggtc gataacaata gcgaaggtga
     8341 atacgccatt attcccgcca gcgttgccga taaaattgct cagcgtgatg ccagcagtat
     8401 tgtgttgcac agcgcgctca gtgccgaaga gcaggatgaa gacgacccgt atgccgactt
     8461 caaagtgcct gatgatttga tgtggtaaac atagataagt cgtgcgcaga tgcctgatgc
     8521 gacgctatgc gcgtcttatc atgcctacgg cctgcgggcg aaatgtaggc ggataaggcg
     8581 ttcacgccgc atccggcagt cgtgcactat tatcaacgca tattcagttt attggcgtgg
     8641 taggcaatat gctcgccaat aaaactggag acaaaataat agctgtggtc gtaaccctcg
     8701 tgataacgga ttaacgtttt gatattcatc tcctggcaga tcttttcaag attttgagtc
     8761 cgcagctgtt ccgcgtaaaa atcatcactc aacccctgat caaccatgat ttccgcaacg
     8821 cgttgacctt gtgaaataag actcaccgga tggtaatcca accaggcctc tttattttca
     8881 ccaagatatg cggcaaaggc ttgctgtccc cacggtactt gcgatgggga gacaatgggc
     8941 gaaaacgccg agacgctgac atattcatct gggttacgta acgccagcac cagcgcgccc
     9001 agtccgccca tagaatgacc agagatagac tttttggccg ttgccggaaa atgattcatc
     9061 actaaatccg gcagctcgtt gcggatatag tcatacattt tgtaatgttc attccacggc
     9121 gcttgcgtcg cgttcaggta aaacccggca ccttgcccga gatcgtaacg gtcggcatct
     9181 gcgacatgac tgcctcgcgg actggtgtcc ggcgcaacaa caataatgtt gtgctcagcc
     9241 gcgtaacgct gcacccccga tttagtaatg aaattctgct cattgcaggt caggcccgaa
     9301 agccagtaca gcaccggtaa tttttcacct gcagcttttg gtgggagata gacgccgaca
     9361 ttcatttcac atttcagtga tgcggaataa tgccgataca cattttgcca gccgccaaag
     9421 ctggcatgtt tttcaatgag ttccatcagt cattatctca cggttaatcc cccggacggc
     9481 tgcccggaga gagtattaag aaccccgtct gaagtggacg gggtaaacca gcgggaaatc
     9541 agtaacgaat tacggttcga atggatttgc cttcatgcat caggtcgaag gcgtcattaa
     9601 tttcatccag gctcatggta tgcgtgacaa acggttccag atcgatatca cctttcatcg
     9661 catcttcaac catacccggt aactgggaac gacctttcac gccgccaaac gcggaacctt
     9721 tccatacgcg accagtgacc aactggaatg gacgggtgga gatttcctga ccggaacccg
     9781 cgaccccgat gatcaccgac tgaccccagc cgcggtgcgc actttccagc gccgcacgca
     9841 tcacgttgac attaccgatg cattcaaagg tatggtcgat accccatttg ttgatatcca
     9901 gcaggacatc tttaatcggt ttgtcgtagt cattcgggtt aatgcagtcg gtggcaccga
     9961 agcgacgcgc cagatcgaat ttcttcgggt tggtatcgat agcgataatc cgtcccgctt
    10021 tcgcctgacg cgcgccctga accactgcca gaccaatcgc gccaagacca aacacagcaa
    10081 cagaatcccc tggctggact ttagctgtgt tgtgtaccgc gccaataccg gtggtcacgc
    10141 cacagcccag caggcagacg tgttcatggt ttgcttctgg attaattttt gccagagaca
    10201 cttccgcgac tacggtgtat tcactgaatg tagaacaccc catgtagtga taaagcggct
    10261 gcccattgta agaaaaacgg gtggtgccgt ctggcatcag gcctttaccc tgggtttcgc
    10321 gaaccgcaac acagaggtta gttttgccag aacgacagaa ctcacactcg ccgcactctg
    10381 cggtgtaaag cgggatcaca tggtcgcctg gtttgacgct ggttacgcct tcaccgactt
    10441 caaccacaac gccagcccct tcgtgaccga gaaccaccgg gaatacacct tccgggtcat
    10501 cgccggagag ggtaaatgcg tcggtatggc aaacgccggt atgggtgact ttaatcagca
    10561 cttcaccttt tttcggtggt gcaacgtcaa tttcaacgat ttccagcggt ttaccgggag
    10621 caaatgcaac ggcagcacgt gatttcatct ctcgctcttc ctcaatatgg taatagattc
    10681 agctatttaa gataggcacg aaccagttca atagtgtcgt caacggattg gctgacttcg
    10741 cggctgtagc agtcatttcg gtcaaacgtt tcccggatat ggctttcaag cacttctgcc
    10801 atcagcccat tagccgcgcc ccgaacggca gcgatctgtt ggagtatggc acggcattcg
    10861 gcatcaccct ccagcgaccg ttccagagca tcaatctgcc ctcgaatacg acgaactcga
    10921 gtaaggacct ttttcttctc ttccggagta ctgggcattt cgcacctcat catctgcacg
    10981 caatatacta taggggggta tgctatatgt caatgcatac ccccctatag tatataacaa
    11041 tggagaataa ttgttgtccg aagcctaacc aggtgaacgg tccctcaaga cagatgggga
    11101 ccgtttatgt gctattttta tgccgcatcc gctaaaacaa acatcccgta aggatggatt
    11161 tcgaggtaat agtgctcacc aatatcgggc tgtaaacggg tggcgtttac ttgtaataaa
    11221 atctcctgtc catgccactc tacggtcact tcgtactgcg gccccatgta ggcgacatgg
    11281 cgaataacac aacgctgact ctcttcgccg tgattgctta aggtgatggc ttccgggcga
    11341 acgccgacgg tgccgctacc ttgcaccggg aaatgcgccg cgcgcggcag gcgataaccg
    11401 tagatatcga catactcttc gctaaagttc gccgggaaca ggttggcgtc gcccataaaa
    11461 cttgccataa agcgggaggc tggctgacga tagagatcct gcggtgagcc aatctgcatg
    11521 atgtgacctt tattcatcac cagcacagtg tcggaaaccg cgaaggcctc actttgatcg
    11581 tgagtcacgt agagcgacgt gatattaaac tgcttttgca gctcgcggat cttatcgcgc
    11641 atgctgcggc gcaggttggc atcgaggtta cttaacggct catcaaacag cagcaccttt
    11701 ggtttgagga tcaacgcgcg ggccagcgcc acgcgctgtt gctgaccacc ggaaatctgg
    11761 tcgacatagc ggtcctcgaa cccttccaga tccaccattg ccagcgcctc tttcacccgc
    11821 tgctttactt cgctacgcga cacgccgagc atctttaacc cgtagccgac gttttcgccc
    11881 agcgacatat gcgggaacag agcgtatgac tgaaacacca tgcagatatc gcgctgctga
    11941 atggaacgat gcgtgacatc ttcgccatca ataaagattt gcccttcact cggtttttcc
    12001 agcccggcaa ccagacgcaa aatggtggtt ttgccgcagc cggaagggcc aagcagcgtc
    12061 accatttgcc cttgtgggat ggtgagattg atattgtcga taaccgtatt actgccgaat
    12121 cgtttagtga cgttgcgcag ttccacaaaa tttttctgac tcatagtgcg ctccattacg
    12181 cctggttttt ggctttagaa cgggagatac gtgcttctcc gatcagccag tcaaagagga
    12241 aaataatcgc cagcatcacc acgatcagaa tcgaaccata ggcgatggct acgccatatt
    12301 cgccgtcttc aacgcggtta agaatgtagg cggttgccac gcgggtgtcc ggcgtgacga
    12361 gaaagacgat ggcgctgacc gtcgtgatgg cccgcacaaa gctgtagatc agggccgaga
    12421 gaatggccgg acgcagcagc ggcagtaaaa tgtgggttat ggttcgcaag gaacccgcgc
    12481 gcaggctgag tgaggcttca tccagcgatt tgtcgatctg ccccaggcct gcaatcccgg
    12541 cgcgaatacc caccggaacg ttacgcatca ccatcgagat aatcacaatt gccgccgttc
    12601 cggtgagata aaccggagcg ctgttaaagg caagaatata cgaaacgccc gccaccgtac
    12661 ccggtacggc aaagcacagc atggtggtga actcgatggt ctttttgcct ttgaattgtt
    12721 gacgcaccac cacccaggcg atgagcaggc caaagatagc ggtaatggga gcggcaatcc
    12781 cggcgtagag cagggtatcc agcagtgaag gccacgcgcc gtcgctcatt ccctgaccaa
    12841 atagcttgat aaagttatcc agcgtcaggg tgtagtccac gccccagttc accgtgaagc
    12901 tgccgtagaa aatgctgccg tagagcaggg cgttaaaggc aatccatatc gcaagtatgg
    12961 cgaccacgct ccacaccagg gtgaccggaa gcggctggac gtcgccacgg taggatttgc
    13021 cggaaaccgt gacgtaagag cgtttaccaa tccacatata ctgtatgcag aacaccagca
    13081 gcgagaacag cagcaggaaa gcgccaaggg tgctggcggc ctgataatcc agctgcgagc
    13141 cggtaatata gaagtaaatt tgcgtcgcca gtacgtcgaa gttaccgcca agtaccagcg
    13201 ggttactgaa atcagccagt gactgtacca cgacgattaa aaaggcattg gcgagcgcgg
    13261 gcttcaacaa cggcacaaac acgccgttaa aggtttgcca gcggctggcg cgcagggtgt
    13321 aggaggcctc ttccagcgag ggatgaatgg ttttaatggc cccgtcgagg atcataaatg
    13381 ccattggcgt gaatgccaga acctgtgcca gccagatccc ggtaaaaccg tacagccagt
    13441 tggtgttggt cagcccaaac cactccacca taaattccgt aacatagccg gaacgcccca
    13501 tcatgagcgt cacgcctaag ccgaccacaa acggtggcgt aacgatgggc aaaatggaaa
    13561 agatgcgacc aataatggcg ctgcgtttag cgatgcgcgt ggtataaatc gccagcacca
    13621 ggccgaagaa agcacagcct atccccaccg ctatcgacaa gacgatagag ttgacgatca
    13681 cctgaacaat atgtgcctgc gagagcacgg tcataaacgc cagcggggcg agctctcccg
    13741 cgtcgttggt gaacatcggg ataaaaatgg cgatgctcgg ccagacgata aaaatgccaa
    13801 tcagcgccac aatcgcaatc agtgagccga tcacgaagcg gtcgccgccc agccattcga
    13861 ggcgggttaa ggccagcgtc ataatcgcgc ctagttcgac aaacagcacg atggcggcgt
    13921 agcctaaacc gcgtccagcc acggtggcgc tgatgacgat aaacgccata cagagcaatg
    13981 cccagccggc atcgaaataa tgacgactac gctgttcgcg cgtggcctga cgaaacgggc
    14041 ggaccagcaa caggctcggg agtaaatacc acagccagct gatattaaaa tgcgaccagc
    14101 cgtacgcccc gaggatctca tcactcgtgg attccagcag gccataatcg aggctccagc
    14161 tcggcaacag gacaaatgcc agccagccca gcaaaaccca aaggaaaatc gcatcccgct
    14221 ttttcacggg atggagtgca agtgtctgtg acatagcagt tccggtatgg ttggccgggg
    14281 caggcgtctg cgctgcaccc ggcaaatgcg cctaatgcgg gtttatttac ccatcttcac
    14341 ttcgctgacc catttattaa tcaatgcctt acgaacatcc gtggaaccat atttatccat
    14401 gtcgtagttg attaatttca ggtcgtcgag cttcagcgaa tttggcgacg tttcggcggt
    14461 ggtattagtc aggatctgat aagacttccc tttcttccac gccagttcct gagcttcttt
    14521 tgataatacc cagtccacaa acagtttggc gttgtcgagg ttgcgcgcgc ctttcagaat
    14581 gctgacgccg ccaatttcgt agcccgtacc ttcacacggg gaaatcaact ccagcggcgc
    14641 gccctgttct ttttccagcg agtagtcatg caggaagcca ataccaatcg ccgtttcgcc
    14701 acgggcggcg ttacgtgccg gggctatgcc tgatttggtg tactgagaga cgttggcgtt
    14761 cagttgtttt agataatcaa aggcctgatc ttccccccac agctgcgcaa aagtagccag
    14821 ggcggtgtag gcagtaccgg aactttgcgg atcggcaatt tgaatttcgc ctttatattc
    14881 cggtttggtg agatctttcc agcacttcgg caccggcagg tttttctctt tcagacgatc
    14941 ggtgtttacg ccaaaaccga ggatccccac ataaacggca gaggacagat tccctttcac
    15001 ttttgccgga tcgcggaatt tctccatgat ctgctcaagg ttaggggatt tatacgcctg
    15061 tagcaggccc atctcgccag cctgcgattg cgggtctaaa gtgccgccgt accagacgtc
    15121 agcctggggg tttttctttt cagcatcgac ttttgccagg gtgcttccgg aaccgttgcg
    15181 gataaacgag gtttttacat catatttctc gccaaacgct ttggtttcgg cctcacacat
    15241 ttcattggtg gcgctgcaat agaccaccag ccggcctttt gcctgcgcgg cacccgttaa
    15301 agtcgccagc gcaacggcag aagcaatcag agtagaggta aaggtcattt tcattgtttt
    15361 atccttcgag cgtgagtggt ggtgatgccc gccatcagta acggcatcag cagtaaaccc
    15421 ataagtactg cggcgaccga gagtaaactg aacattcccg gccagccgta gcgttcgatg
    15481 accagcgaca gcggccagcc tgccagcgcc gcgccaaggt aagcgaacag gccgagaaat
    15541 ccggtgattg aacccgccgc agctttgtgc ccacactcca ccgccgcaag tccaatcagc
    15601 atttgtgggc caaagacgaa aaagcccacg gtaaaaaagc acactgccag cagagcgtag
    15661 tgatgaaccg gtgccagcca cagtgcggcg actgacacca tcaaccccaa cgtgaacagc
    15721 aaaatcattg gcgctcgttg cccgctaaac agcaaatccg atccccatcc ggcaaataac
    15781 gcgccgagta agccgcccac ttcaaacagc attaccgtgg cgttggcgct gagcaggttg
    15841 acaccgtggc tttccgtcag ccagatattg ccccagtcgt taagcgcaat ccggatcaca
    15901 taaaccagta catatgagac gcccagtagc cagatcagcg gattttgcag catggtggtg
    15961 cgcaacattt gccacagccc catcggcgga ctttgttgtt cctgacgtaa ctccagcgga
    16021 tcgtgacgcc agtgaccaac tgtcggtaat ccctcttcct ggggcgtgcc tttgagttgt
    16081 agcgttaacc agatccctaa tgccatgctg attatccccg gtgtcagcat cgcggcctgc
    16141 cagccccacc agtgggcggc aaaggcgcta atcagcggaa taatcgctcc gccaatgttg
    16201 atcgacatat tccagcagcc ccaccagaaa ccgcgctcat tgcgtgagta ccagtgggtc
    16261 agcaagcggg cacacggtgg ccatccccag ccctgaaaaa aaccgttcag tgtccagacc
    16321 acaagcagaa gcgtaagtga ttcgccaaag gcaaacacca cgttcaataa cccggtggcg
    16381 aacaagccga cgcccataaa accgcgttgt ccgtggctgt catgccacag tccggcggta
    16441 aatttcgaca atccgtagct gagataaaac agcgaaccca gcaggccaat gtcgccttta
    16501 tccagtccga ggtcggtttg tagcgccgga aggacgtaat tcacgctttt tcgcgtcaga
    16561 taaaatgcgg cgtagccaat aatcatgcac atcagcaatc gcggacgtaa tgtccggtag
    16621 cattgattaa tttctctcgc tgaacgcgcg tgcatggttg tctccatgtt gctgactata
    16681 tgaaaatgaa agtgaaaggg gatgagagaa aatccgggat acctaagact ttttcctggt
    16741 tatccggcga tttgttgcaa aaatgtgggc aagttaacaa ttacccgcgt gccgtgctgt
    16801 ttttcgagcg ttaagtcgcc gcccagggcg ctgacgcgct cacgcatccc ctgaatgcca
    16861 aatccagcca ttttcccgct ggcgatcccc acaccgttgt cgctgacttc cagatgcaat
    16921 acctctccct gctggcgcag gataatcgtc acttcgctgg cttcggcgtg tttacagatg
    16981 ttattgagca actcctgcaa taaccgatac aaggtaaagc gcaccgtttc gttttctggt
    17041 gtggagttca gttgataggc aaactggcaa tgaatgccgc gttcactaaa ggcaaattca
    17101 ttaatcagat gcagcaacgc ttcgcgaaat gtcagttcat caagcgcggg aggccgcaac
    17161 tggcgcagca attggcgggt ggagagatga atgcggcggg cgagctcatt gatttggctg
    17221 gctgcggatt gaatttgcgc cggatcacgc gcccgcttca ccagttgcga ctggatctga
    17281 atggcggtga tgttctgacc gatttcgtcg tgcagctctc gcgccaggtt ttttcgcgtg
    17341 tcctcctcgg tgtggatcag cttttccgcc aatgcccgcc gcgctgccag ctcggcctcc
    17401 agccgttgtc ggtaatggtg caggtttagc gccagatgtt gctggcgact gatggcaatg
    17461 cccaggccaa ttcccagcaa ggcctgggtc gcgaggaaaa tctccagctc caccaggtta
    17521 ctaaagccca cgccaatctg gcgggcgatg gtgatcatca tgctgcccaa aagcccggag
    17581 agtacgccac cttgccagcc aaatttccac gccatcacca cattgggcaa aaagaccacg
    17641 atcaacagca gacgttcaat ttcaggtgac agcaccatct gagtgccgat accaataata
    17701 aaaaacaggc tacaccagat gattaaagag gtacgcaagg ggggattatg cgtatcgagt
    17761 cccaggagct ggtagcgatg ctgctggcgc agaaactcaa agataagata gacaaacggc
    17821 gtcagtaata cgccgccggt aaatgaggtc aaacccggta gcattattgg gcctgacata
    17881 aaaggggaga gcagggcggt attcaacaat gaagctgccg tgacagtggc taacagcaac
    17941 gtcagacgct gccagtaaag tggaaaacgg tgccagaagt tttgcgcaag gagggcagga
    18001 aacaggctaa taaccggcgt tgccagcatg agataaccgt tcaatagctg ttcactatgg
    18061 agccagaaca tcatcagcaa aggggggagc accaacgcag gccagtagcg gcgagagagc
    18121 aaaataacaa gggcgagata aactccctgc gggagaaaca acaccgcctg ttgaccgtta
    18181 ttggtcagat aaaagctgag cgtccacagc atcagccagc cggttcccca ggctagcaca
    18241 ataaacagca aaatcaccca atgacgaaga ttgcgcgaca ttaatgcccc gccagcagat
    18301 ggtggtcgag cgcaaaatgt accagttcaa tggtgctatg gcagttcagt tttcccagca
    18361 cattggcgcg atgcacatgt accgttttat gactgagttc gagtttaaag gcgatctctt
    18421 taacgctgtc tcctttgacc agtaactcaa acacttcacg ttctcgtggg gtgagaattt
    18481 ccagcgcctg ggcgggttgc ccgccgccgc gtaaagcccg aatcgcatcg gcacagagat
    18541 aatgcccgcc cagcccgacc gagcgcaccg cctgtactaa ctcttccggc ccacagcgtt
    18601 tggttaaata cccgctggca ccggcatcca gcgcgctttg cacaaagatt ggcgcgtcat
    18661 aaatgctaag aataatggcg cgaaattgcg gtttttgtgc acgcaggcgc ttaagcaaac
    18721 tgaggccgtt ttcgtccggc atggcaatat caatgacggc aacattgacg tcatcacgga
    18781 ttaatgccga ccatgcctgc gcggcactac tgtactgacc aatgacctcc agatcgtctt
    18841 ccagagagag taattgtgcg aagccagagc gcaccacaac atggtcatcc accagcacta
    18901 cccgaatcat acttttcttc tcttattatc taattctcct atcatgcatc acggcgcggc
    18961 gctggcaatc aaccagcgac agatatggaa tggatgtaac aaaatggcaa taatttgccg
    19021 gagcgcgcac atcttatctg gcttgtgaac cggcgggata cgttcgccaa aaccgcaaaa
    19081 tgcgcaaact cgctaaccat tgccgatttt tttctaaaaa cgctttgttc ttaacaagac
    19141 cttttaattc ctttatcaaa ctgacccgca acgaaatact gctcccataa caagacaggg
    19201 gagcagacaa tcatggcaat ttcatcgcgt aacactcttc ttgccgcact ggcattcatc
    19261 gcttttcagg cgcaggcggt gaacgtcacc gtggcgtatc aaacctcagc cgaaccggcg
    19321 aaagtggctc aggccgataa cacctttgct aaagaaagtg gagcaactgt ggactggcgc
    19381 aagtttgaca gcggagccag catcgtgcgg gcgctggctt ctggcgacgt gcaaatcggc
    19441 aaccttggtt ccagcccgtt agcggttgca gccagccaac aggtgccgat tgaagtcttc
    19501 ttgctggcgt caaaactggg taactccgaa gcgctggtgg taaagaaaac tatcagcaaa
    19561 ccggaagatc tgattggcaa acgcatcgcc gtaccgttta tctccaccac ccactacagc
    19621 ctgctggcgg cgctgaaaca ctggggcatt aaacccgggc aagtggagat tgtgaacctg
    19681 cagccgcccg cgattatcgc tgcctggcag cggggagata ttgatggtgc ttatgtctgg
    19741 gcaccggcgg ttaacgccct ggaaaaagac ggcaaggtgt tgaccgattc tgaacaggtc
    19801 gggcagtggg gcgcgccaac gctggacgtt tgggtggtgc gcaaagattt tgccgagaaa
    19861 catcctgagg tcgtgaaagc gttcgctaaa agcgccatcg atgctcagca accgtacatt
    19921 gctaacccag acgtgtggct gaaacagccg gaaaacatca gcaaactggc gcgtttaagc
    19981 ggcgtgcctg aaggtgacgt tccggggctg gtgaagggga atacctatct gacgccgcag
    20041 caacaaacgg cagaactgac cggaccggtg aacaaagcga tcatcgacac cgcgcagttt
    20101 ttgaaagagc agggcaaggt cccggctgta gcgaatgatt acagccagta cgttacctcg
    20161 cgcttcgtgc aataaaagga ggcgcagatg ctgcaaatct ctcatcttta cgccgattat
    20221 ggcggcaaac cggtactgga agatatcaac ctgacgctgg aaagcggcga gctactggtg
    20281 gtgctggggc cgtccggctg cggtaaaacc accctgctga atttgattgc cggttttgtg
    20341 ccttatcagc atggcagcat tcaactggcg ggtaagcgta ttgaggggcc gggagcagag
    20401 cgtggcgtag tttttcagaa tgaagggcta ctaccgtggc gcaatgtaca ggacaacgtg
    20461 gcgttcggcc tgcaattggc aggtatagag aaaatacagc gactggaaat cgcgcaccag
    20521 atgctgaaaa aagtggggct ggaaggcgca gaaaaacgct acatctggca gctttccggt
    20581 ggtcaacgtc agcgggtggg gattgcccgc gcgctggcgg cgaatcccca gctgttatta
    20641 ctcgacgaac cgtttggtgc gctggacgcc ttcacccgcg accagatgca aaccctgctg
    20701 ctgaaactct ggcaggagac gggcaagcag gtgctgttga ttacccacga tatagaagaa
    20761 gcggtgttta tggcgactga actggttctg ctttcacccg gccctggccg cgtgctggag
    20821 cggctgccgc tcaactttgc tcgccgcttt gttgcgggag agtcgagccg cagcatcaag
    20881 tccgatccac aattcatcgc catgcgcgaa tatgttttaa gccgcgtatt tgagcaacgg
    20941 gaggcgttct catgagtgtg ctcattaatg aaaaactgca ttcgcggcgg ctgaaatggc
    21001 gctggccgct ctcgcgtcag gtgaccttaa gcattggcac gttagcggtt ttactcactg
    21061 tatggtggac ggtggcggcg ctgcaactga ttagcccgct gtttttgccg ccgccgcaac
    21121 aagtactggc aaaactgctc accattgccg gaccgcaggg atttatggac gccacgctgt
    21181 ggcagcatct ggcagccagc ctgacgcgca ttgtgctggc gctactcgcg gcggtgttga
    21241 tcggcattcc ggtcgggatc gccatggggc taagcccgac ggtgcgcggc attctggatc
    21301 cgataatcga gctttatcgt ccggtgccgc cgctggctta tttgccgctg atggtgatct
    21361 ggtttggtat tggtgaaacc tcgaagatct tactgatcta tttagcgatt tttgcaccgg
    21421 tggcgatgtc ggcgctggcg ggggtgaaaa gcgcgcagca ggttcgcatt cgtgctgccc
    21481 agtcgctggg tgccagccgt gcgcaggtgc tgtggtttgt cattttgccc ggcgcgctgc
    21541 cggaaatcct caccggatta cgtattggtc tgggtgtggg ctggtctacg ctggtggcgg
    21601 cggagctgat tgccgcgacg cgcggtttag gatttatggt tcagtcagcg ggtgaatttc
    21661 tcgcgactga cgtggtgctg gcggggatcg cggtgattgc gattatcgcc tttcttttag
    21721 aactgggtct gcgcgcgtta cagcgccgcc tgacgccctg gcatggagaa gtacaatgag
    21781 tgaacgtctg agcattaccc cgctggggcc gtatatcgga gcacaaattt cgggtgccga
    21841 cctgacgcgc ccgttaagcg ataatcagtt tgaacaactt taccatgcgg tactgcgcca
    21901 tcaggtggtg tttctgcgcg atcaagccat tacgccgcag cagcaacgcg cgctggccca
    21961 gcgttttggc gaactgcata ttcaccctgt ttaccctcat gccgaagggg ttgacgagat
    22021 catcgtgctg gatacccata acgataatcc gccagataac gacaactggc ataccgatgt
    22081 gacatttatt gaaacgccac ccgcaggggc gattctggca gcgaaagagt taccttcgac
    22141 cggcggcgat acgctctgga ccagcggtat tgcggcctat gaggcgctct ctgttccctt
    22201 ccgccagctg ctgagcgggc tgcgtgcgga gcatgatttt cgtaaatcgt tcccggaata
    22261 caaataccgc aaaaccgaag aggaacatca acgctggcgc gaggcggttg cgaaaaatcc
    22321 gccgttgcta catccggtgg tgcgaacgca tccggtgagc ggtaaacagg cgctgtttgt
    22381 gaatgaaggc tttactacgc gaattgttga tgtcagcgag aaagagagcg aagccttgtt
    22441 aggcttttta ttcgcccata tcactaaacc ggagtttcag gtgcgctggc gctggcaacc
    22501 gaatgatatt gcgatttggg ataaccgtgt gactcagcac tatgccaatg ccgattacct
    22561 gccacagcgg cggattatgc atcgggcgac gattcttggg gataaaccgt tttatcgggc
    22621 ggggtaatac gagagtggac ggtcctctcg cccccttggg gagagggtta gggtgagggg
    22681 gcgttcaccg tgctttcaac aggttaactc cccctttctg agaggaaaca aaattaacgc
    22741 agaatcttct tctcagccaa atccagcgca aagtagctga aaatcagatc cgcacccgca
    22801 cgcttaatcg aacctaagct ttcgagcacg actttctctt catctatagc acccgccagc
    22861 gcggcgaact taatcatcgc atactcaccg ctcacctgat acgcgccaat cggcaattca
    22921 gtacgttcac gcagctcacg cacgatgtcg aggtacgctc cagcaggttt aaccatcagg
    22981 cagtctgcgc cctgggcttc atccagcagt gactcacgaa tcgcctcacg acggttcatt
    23041 gggttcatct gatagctttt gcggtcgcct tttaatgcgc ttccggcagc ttcacggaac
    23101 ggaccataaa aggaggaggc gaacttggtc gaatacgaca taatcgccgt atctttaaag
    23161 ccagcagcgt ccagcgcctg gcgaatcgcc tgtacctggc catccatcgc ggcggaaggg
    23221 gcgatgaagt ctgcacctgc agcagctgca accacggctt gcttgcctaa attttccaga
    23281 gtcgcgtcgt tgtcgacgcc atgctcgcac agcacaccgc agtgaccgtg agaagtgtat
    23341 tcacagaagc aggtgtctga catgacaatc atttctggca ccgtctgctt gcagatgcgc
    23401 gacatacgcg ctaccagtcc atcttcccgc caggcatcgc tgccggtttc atcggtatgg
    23461 tgagagatgc cgaaagtcat cacggaacga ataccggcgt tggcgatgcg ttcaatttcg
    23521 cgtgccagat gtttctctgg aatgcgcatc acgccaggca tggcttcaac ggctttgtag
    23581 tcgtcaattt cttcttcaac aaagatcggc aacaccaggt cgttaaggct aagtgttgtc
    23641 tcttcaaaca tagcgcgcag cgcaggagat ttgcgcaggc gacgagggcg ttggattaag
    23701 tctgtcatgg tctgcctgat gtttgtggaa ttaaggtgca cagtatacct gaagcggggc
    23761 tggagtgttt tacgaaagtt gtcacgatgt tgacaagaag agaatgaaag agagaccagg
    23821 gcatctttgt aatagaaata gcaataactt c
//
LOCUS       DBFIFI010000057        23783 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.057, whole genome shotgun sequence.
ACCESSION   DBFIFI010000057 DBFIFI010000000
VERSION     DBFIFI010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23783
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.057"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(67..1257)
                     /gene="bcr"
                     /locus_tag="N7B39_002131"
     CDS             complement(67..1257)
                     /gene="bcr"
                     /locus_tag="N7B39_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HGU2422114.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILAVAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(1285..1980)
                     /gene="rsuA"
                     /locus_tag="N7B39_002132"
     CDS             complement(1285..1980)
                     /gene="rsuA"
                     /locus_tag="N7B39_002132"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HGU2422115.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            2129..3889
                     /locus_tag="N7B39_002133"
     CDS             2129..3889
                     /locus_tag="N7B39_002133"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGU2422116.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAATRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            4014..4298
                     /gene="rplY"
                     /locus_tag="N7B39_002134"
     CDS             4014..4298
                     /gene="rplY"
                     /locus_tag="N7B39_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HGU2422117.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNIQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(4437..5444)
                     /gene="yejK"
                     /locus_tag="N7B39_002135"
     CDS             complement(4437..5444)
                     /gene="yejK"
                     /locus_tag="N7B39_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HGU2422118.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            5626..5853
                     /locus_tag="N7B39_002136"
     CDS             5626..5853
                     /locus_tag="N7B39_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HGU2422119.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            5873..7633
                     /gene="yejM"
                     /locus_tag="N7B39_002137"
     CDS             5873..7633
                     /gene="yejM"
                     /locus_tag="N7B39_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HGU2422120.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            7708..7784
                     /locus_tag="N7B39_002138"
     tRNA            7708..7784
                     /locus_tag="N7B39_002138"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7742..7744,aa:Pro,seq:ggg)
     gene            complement(7885..10476)
                     /gene="yejO"
                     /locus_tag="N7B39_002139"
     CDS             complement(7885..10476)
                     /gene="yejO"
                     /locus_tag="N7B39_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HGU2422121.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVVLE
                     KDIQLVYGTANNTKINPGGEQHIKEFGISSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGAANDPTIKGGRLIVEKDGITVLAAIEKGGLLEVKEGG
                     LAIAVDQKAGGAIKASTRVMEVFGTNRLGQFEIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTRVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     LRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMDESVTRS
                     SVTASAENFTTLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTAMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNVDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTRGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            10796..11443
                     /gene="narP"
                     /locus_tag="N7B39_002140"
     CDS             10796..11443
                     /gene="narP"
                     /locus_tag="N7B39_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HGU2422122.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLHEREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(11478..12530)
                     /gene="ccmH"
                     /locus_tag="N7B39_002141"
     CDS             complement(11478..12530)
                     /gene="ccmH"
                     /locus_tag="N7B39_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HGU2422123.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDATLGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(12527..13084)
                     /gene="dsbE"
                     /locus_tag="N7B39_002142"
     CDS             complement(12527..13084)
                     /gene="dsbE"
                     /locus_tag="N7B39_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HGU2422124.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(13081..15024)
                     /gene="ccmF"
                     /locus_tag="N7B39_002143"
     CDS             complement(13081..15024)
                     /gene="ccmF"
                     /locus_tag="N7B39_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HGU2422125.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(15021..15500)
                     /gene="ccmE"
                     /locus_tag="N7B39_002144"
     CDS             complement(15021..15500)
                     /gene="ccmE"
                     /locus_tag="N7B39_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HGU2422126.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(15497..15706)
                     /gene="ccmD"
                     /locus_tag="N7B39_002145"
     CDS             complement(15497..15706)
                     /gene="ccmD"
                     /locus_tag="N7B39_002145"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HGU2422127.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(15703..16440)
                     /gene="ccmC"
                     /locus_tag="N7B39_002146"
     CDS             complement(15703..16440)
                     /gene="ccmC"
                     /locus_tag="N7B39_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HGU2422128.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(16482..17144)
                     /gene="ccmB"
                     /locus_tag="N7B39_002147"
     CDS             complement(16482..17144)
                     /gene="ccmB"
                     /locus_tag="N7B39_002147"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HGU2422129.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(17141..17764)
                     /gene="ccmA"
                     /locus_tag="N7B39_002148"
     CDS             complement(17141..17764)
                     /gene="ccmA"
                     /locus_tag="N7B39_002148"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HGU2422130.1"
                     /translation="MGMLEVRELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAV"
     gene            complement(17777..18379)
                     /gene="napC"
                     /locus_tag="N7B39_002149"
     CDS             complement(17777..18379)
                     /gene="napC"
                     /locus_tag="N7B39_002149"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HGU2422131.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(18389..18838)
                     /gene="napB"
                     /locus_tag="N7B39_002150"
     CDS             complement(18389..18838)
                     /gene="napB"
                     /locus_tag="N7B39_002150"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HGU2422132.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(18835..19698)
                     /gene="napH"
                     /locus_tag="N7B39_002151"
     CDS             complement(18835..19698)
                     /gene="napH"
                     /locus_tag="N7B39_002151"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HGU2422133.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(19685..20380)
                     /gene="napG"
                     /locus_tag="N7B39_002152"
     CDS             complement(19685..20380)
                     /gene="napG"
                     /locus_tag="N7B39_002152"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HGU2422134.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLVVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(20387..22873)
                     /gene="napA"
                     /locus_tag="N7B39_002153"
     CDS             complement(20387..22873)
                     /gene="napA"
                     /locus_tag="N7B39_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HGU2422135.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQDVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(22870..23133)
                     /gene="napD"
                     /locus_tag="N7B39_002154"
     CDS             complement(22870..23133)
                     /gene="napD"
                     /locus_tag="N7B39_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HGU2422136.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(23123..23617)
                     /gene="napF"
                     /locus_tag="N7B39_002155"
     CDS             complement(23123..23617)
                     /gene="napF"
                     /locus_tag="N7B39_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HGU2422137.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
BASE COUNT         5816 a         6765 c         5863 g         5339 t
ORIGIN      
        1 atgatagatt tgtggttgac atgcatcaac aaaggaagcc ttttagcttc ctcgttgtgc
       61 aatagatcac cgttttttcg gccgactggc gtacagacag aagagaatgg agctggttgc
      121 gcagaatgca attgaccaaa tcatcggcca tgcagagtta aaggtcgcaa gagaaagcaa
      181 tgcgccaaca attgccccta tgccaaaacg gaaggttcct gccagcgaag atgccgttcc
      241 cgccatatgg ggaaactcat caagaatgac cgccatcgca ttggatgaca ccatcgacac
      301 gcagcccaca aacgccgcaa cgccaaccac cagcgaccaa aatcccagac ccagcagcgc
      361 actgatgacc atccacgctg ccataataaa ttgtatccac aaccccgagc ggaacatatt
      421 taacgcgcca atacggcgga cgaagcggct attaaagatg gtcatcacga acaggaaaac
      481 aatgtttagt gcaaagtaat aaccaaagtt ttccggcgcg acgtggttaa tttcaatgta
      541 gacaaacggt ccggcgctta agaacgagaa catcccggca aagctgaaac cactggccag
      601 catataactc aggacacgct tatgacggaa cagcgccgca aagttaccaa tagtggtacg
      661 aatgtgaaaa ggctggcgac gctccggtgg taaggtttct ttgatcagaa agaagatcat
      721 tgccgaagcc agaatcgccg ccacggcgag gatccagaag atgtaatgcc agctcagcca
      781 caccagcacc cagccgccaa ctatcggtgc catcagcggt gcaatggtcg tcaccagcat
      841 gacaaacgac atcatccgcg agaactcttc tttcgggtag atatcacgca tcaaagcgtt
      901 gataaccacg ctggccgcag ccgccgccag cccgtggaag aaacgcatca caatcagctg
      961 atcgatggtt tgtgccaacg cacacgccac cgcggcggcg gcaaacacca gcgtaccgcc
     1021 aagaaccacc ggcttacgcc cgaagctgtc tgccatcggc ccgtagatta actgccccaa
     1081 cgcaaagccc agaatataag tactgagggt catctgcgta ctgcccgccg gtacgccaaa
     1141 ctgcgctgaa attaccggta gcgcgggcag atacatatca atcgacagcg gcatcaacat
     1201 ggccagcagg ccaaggataa aaacaatagc aaacgacgaa tgctgtcggg tggtcacaac
     1261 gggctcctga aagtcattga aaagttagac gacgctggca atttcttctt cagttaacgg
     1321 acgatattca ccgggggcta aatcagcatc cagcgtaatg ccgccaatac gttcacgatg
     1381 cagctcaacc acatggttac ccacggcggc gaacatgcgt ttcacctgat gataacgccc
     1441 ttcgctgatg gtcagacgaa cctgcgttgg ggtaatcact tcaagcaccg ctggcttagt
     1501 gagatctttt tcgttatgca gctgcacgcc tttagcaaat tgctctgccg tatcgtcagc
     1561 tacgggtgat tccagcgtca ccagataggt cttctcgcaa tgatggcgcg gagaagtaat
     1621 gcggtgcgac cactgaccat catctgtcat tagtaccaga ccggtggtat cgatatccag
     1681 ccgccctgcg gcgtgcagtt tccacgccac tggttcatct agaaaataga gcaccgttgg
     1741 gtgatcaggg tcgtccgtgg agcaaacata gccctgaggc ttattgagca tgaagtaacg
     1801 tggaccgtgt tgctgcgcca gcgggttgcc atcgtaagcg acatcatgtt caggaagcag
     1861 tttgaacgct gcattacgga cgatttcgcc atcgacggtg acacgattgc cgcggatttc
     1921 acgcccggca atagcacggc taacgccgag ttgctgtgcg ataaatttat caagtcgcat
     1981 gtgtgtgatt ttgcctgtaa aaacggaggt cgggcattga cccgaaaatc tgaactgttg
     2041 tctgcccagt atagcggtct aataacttcc ctcaagggaa aaagaaccgt ggcatactat
     2101 tagcagaata atctacctac gcgagaccat gatttttaca ctccgcccat atcagcaaga
     2161 agccgtggat gccacgctca accattttcg tcgtcataaa acccctgccg ttatcgtgct
     2221 gcccaccggt gcaggtaaaa gcctggtgat agcggaactt gcgcggctgg ctcgtggtcg
     2281 cgtgctggtg ctggcacacg ttaaagaact ggtggcgcaa aaccatgcaa agtatcaggc
     2341 gctggggctg gaagccgata tttttgccgc cgggctaaag cgcaaagaga gccacggtaa
     2401 agtggtattt ggcagcgtgc agtcggtcgc ccgtaatctt gatgcctttc agggtgaatt
     2461 ttcgctgttg attgtcgatg aatgtcaccg tattggtgac gatgaagaga gccagtatca
     2521 gcaaatcctc actcacctga caaaagtgaa tccccactta cgcctgctgg ggctgactgc
     2581 cacgcctttt cgactgggca aaggctggat ctaccagttc cattatcacg gcatggtacg
     2641 cggcgatgag aaagcccttt tccgtgactg catttatgaa ctgccgctgc gttatatgat
     2701 taaacacggc tatctgacgc cgccagaacg actggatatg ccagtagtgc aatacgattt
     2761 cagccgcttg caggcacaga gtaacgggct attcagcgaa gccgatctca accgcgagct
     2821 aaaaaaacaa caacgtatta ccccgcacat catcagccag attatggagt ttgctgcaac
     2881 gcgcaaaggg gtgatgattt tcgccgccac cgttgaacac gcaaaagaga ttgtgggatt
     2941 actacccgcc gaagatgctg cactgattac tggcgacacc cccggcgctg agcgcgatgt
     3001 gttaattgaa gattttaaag cccagcgttt tcgctatctg gtcaacgtcg cggtactgac
     3061 caccggattt gacgccccgc acgtcgatct tatcgccatt ctgcgcccta ccgaatcagt
     3121 gagtctttac caacaaattg tcgggcgcgg tctgcgtctc gctcctggca agactgattg
     3181 cttaattctt gattatgcgg gtaatcctca cgatctctac gcgccggaag ttggtacacc
     3241 gaaaggcaaa agtgacaacg ttccggtaca ggttttctgc cctgcctgcg gttttgccaa
     3301 caccttttgg gggaaaacga ccgccgacgg gacattgatt gaacactttg gtcgccgctg
     3361 tcagggatgg tttgaagatg acgacggtca tcgcgaacag tgtgacttcc gtttccgttt
     3421 taaaaattgc ccgcaatgta acgcagaaaa tgatattgcc gctcgccgct gccgcgaatg
     3481 tgacacggta ctggttgatc cggatgatat gttaaaagcg gcgctacgac tgaaagatgc
     3541 gctggtatta cgctgtagcg gcatgtcttt gcagcatggg cacgacgaga aaggcgaatg
     3601 gttgaaaatc acctattacg atgaagacgg cgcggatgtg agtgagcgtt tccgtctgca
     3661 aacgcccgcc cagcgaactg ccttcgagca gctttttatc cgcccgcata cgcgcacacc
     3721 gggcatccct ctgcgctgga tcaccgccgc cgatatcctc gcccagcaag ccttattgcg
     3781 acacccggat tttgtcgttg cccgcatgaa aggtcagtac tggcaagtgc gtgaaaaagt
     3841 gttcgattac gaaggtcgtt ttcgtcgggc gcacgaatta cgcggttaat ggtagttttg
     3901 attgatgtat aaggcgattg agtatagaat ctcgcccgct tttgcatacg caaagcagat
     3961 cacttacctg ttgctgggtc gcctgtagca ggattaattt aagagagaaa gaaatgttta
     4021 ctatcaacgc agaagtacgt aaagagcagg gtaagggtgc gagccgccgc ctgcgtgccg
     4081 ctaacaagtt cccggcaatc atctacggtg gcaaagaagc accgctggct atcgagctgg
     4141 atcacgacaa agtcatgaac attcaagcta aagctgaatt ctacagcgaa gttctgacca
     4201 tcgttgttga cggtaaagaa atcaaagtta aagctcagga cgtacagcgt cacccgtaca
     4261 aaccgaagct gcagcacatc gacttcgttc gcgcttaatt gctgaataag ttgtaaaaaa
     4321 ccccgctccg gcggggtttt ttgtatctgc agattatgcc tgattacgat attgctattt
     4381 tttgcaggcc agataaggcg ttcacgccgc atctggcatt cataacacaa aaccgattaa
     4441 ttgccgccgg acgtccggcg ctgcaattga tcgcgcaaat tcggcggcgt gcctttaata
     4501 gtcagggtat cggtcgccgg atcccagaaa atccgttcgc ccaacagcat cgcatcaaaa
     4561 ttaatagtca agccaccgcc gctaccggca aacttcgtca actgacgcag tgtgctacgg
     4621 tctgccggga agctctcttc cagttcgtag cctttttcag ccgcaaactc cgtgaagctc
     4681 acttcgctga cgcctgccag ctctttcgac agcgattcca gttcgatctc ttcgcctgcc
     4741 tgcagctgct cattacagta gctataaacc tgctggcgca cgttctgtcg ctctgcttta
     4801 tccagttgtg cttcggcagt gaaatcatca accgcttgca acagcccacg gttttgcgct
     4861 ttcgcgttca atccttcgct ggccccgagg aaatccataa agaaatccgc cactttgcgc
     4921 cctactcgcc ctttcaggaa agtgagatag cgggtcgact ctggattggt ttcccattcg
     4981 gttaaatcga tacgcgcaac aatatccgca tgattgatat caagataatg ggttgggttg
     5041 atgtcgagat tttcgttaac gcgcatactg ctcaggttgc tcaggactgc caccagcaga
     5101 tactccaccg ccagataacg gtagtggcaa aacagcacga atccaccgtc agcaaaagga
     5161 tatttcgcta attcgtcacg caggcgaccg gttgccgcgc ggctaaatgc cagaaagtcc
     5221 tcttcaccct gacgctgtaa gcgcagcgtc tgcgccagtt cactctcttc gctaaacaag
     5281 ccgtaagctt tatttttggc gctatagacc cgatgcagtt ctgctaccat ctcaacgacg
     5341 gtttctgtcg gttccagcaa tgaatcgcgc aacaccagct caaggttctg ctcatcacgc
     5401 ttgataagct ggtgcagagc aatctggttg atatccagac tcatgataaa ctctccttta
     5461 agaccgggcg gtattcaacc accgccggtt aagcgacgca ataaaagatg gcggtcattt
     5521 tacagaaggc ggcgtaacga gaatgattta acgcataaaa aagcagaaaa aaaaccgttg
     5581 ctacggtaat atgttgccct ttcatcaact aactgatttc gatttatgcc acaaatttcc
     5641 cgctacagtg atgaacaggt tgaacaattg ctcgcagagc tgctcaacgt actggaaaaa
     5701 cataaggctc cgaccgacct ttccctgatg gtgttaggca acatggtcac taaccttatc
     5761 aacaccagca ttgccccggc ccaacgccag gcaattgcca actcttttgc ccgcgcctta
     5821 cagtcctcta tcaacgaaga caaagcgcac taagggaaac agataacagg ttatggtaac
     5881 tcatcgtcag cgctaccgtg aaaaagtctc ccagatggtc agttgggggc actggtttgc
     5941 actgttcaat attctgcttt cgctcgtcat tggcagccgt tacctgttta tcgccgactg
     6001 gccgaccacg cttgctggtc gcatttattc ctacgtaagc attatcggcc atttcagctt
     6061 cctggtgttc gccacctact tgctgattct cttcccgctg acctttatcg tcggctccca
     6121 gaggctgatg aggtttttgt ccgtcattct ggcaacggcg ggaatgacgc tattactgat
     6181 cgatagcgaa gtctttactc gtttccatct ccatcttaat cccatcgtct ggcaactggt
     6241 tattaaccca gacgaaaatg agatggcgcg cgactggcag ctgatgttca tcagcgtgcc
     6301 ggttatttta ttgcttgaac tggtgtttgc gacgtggagc tggcaaaagc tgcgcagcct
     6361 gacgcgtcgt cggcgcttcg cgcgtccgct ggccgcattc ttatttatcg cctttatcgc
     6421 ctcgcatgtg gtgtatatct gggccgatgc caacttctat cgccctatca ccatgcagcg
     6481 cgctaacctg ccgctttcgt acccgatgac ggcgcgacgt tttcttgaga agcatggcct
     6541 gcttgatgcg caggagtatc aacgccgtct tattgagcaa ggtaatccag acgccgtttc
     6601 cgttcagtat ccgttaagcg aactgcgcta tcgcgatatg ggcaccgggc agaatgtctt
     6661 gttgattact gtcgatggcc tgaactactc acgcttcgag aagcagatgc ctgcgctggc
     6721 aggttttgct gagcaaaata tttcgttcac gcgccatatg agctccggca acactacaga
     6781 caacggcatc tttggcctgt tctatggcat ctcgccgagc tatatggacg gcattctgtc
     6841 gacccgtacg cctgcggcat taattactgc gcttaatcag caaggctatc agctggggtt
     6901 attctcatca gatggcttta ccagcccgct gtatcgccag gcattgttgt cagatttctc
     6961 gatgccgagc gtacgcaccc aatccgacga gcagaccgcc acgcagtgga tcaactggct
     7021 gggacgctac gcacaagaag ataaccgctg gttctcgtgg gtctctttca atggtactaa
     7081 cattgacgac agcaatcagc aggcatttgc acggaaatat agccgggcgg caggcaatgt
     7141 cgatgaccag atcaaccgcg tgctcaatgc actgcgtgat tctggcaaac tggacaatac
     7201 ggtggtgatt atcactgccg gtcggggtat tccactgagc gaagaggaag aaacctttga
     7261 ctggtcccac ggtcatctgc aggtgccatt agtgattcac tggccaggca cgccggcgca
     7321 gcgtattaat gcgctgactg atcataccga tctgatgacg acgctgatgc aacgcctgct
     7381 acatgtcagc acacctgcca gcgaatattc gcaaggtcag gatttgttca accctcaacg
     7441 ccgtcattac tgggttaccg cagcggataa cgatacgctg gcaattacca ccccgaaaaa
     7501 gacgctggtg ctgaacaata acggtaaata ccgcacttac aacttacgtg gtgaaagagt
     7561 gaaagatgaa aaaccacagt taagtttgtt attgcaagta ctgacagacg agaagcgttt
     7621 tatcgctaac tgattaatta taaatcagtt agcgaaatat cttacttgca atcggtgtgg
     7681 aaaacggtag tattaacaac cacgagtcgg cacgtagcgc agcctggtag cgcaccgtca
     7741 tggggtgtcg ggggtcggag gttcaaatcc tctcgtgccg accaaaaatc ccaaaaaaac
     7801 caaccgtaaa ggttggtttt tttatatctg catttaattc gataaacaga ccgcgacaca
     7861 tcagggcctg tttattgtct gttatcagaa cgtccagacc acacccgcct gggtattcca
     7921 cggcgattct acccctgcgc cattgccata gctgactgac aaatgaccgc ttaacgtcgg
     7981 ggtaaacgat gaccttatcc cagcctgata aacgccacgg gttccagcca catcattatt
     8041 aaatttgcca tcgtcattaa ctttcacctg gttagaatcg gcgtattcct gacgcacggc
     8101 ggctttcagc cagggttcca gcgtcgtacc gttttgcagg tccatgtgat agcttaccgc
     8161 cgttcccgct tcagcgcgta atatccgggt atttcccaca tccgcgcgca tgccgtttga
     8221 taacgtgtag tcctgaccat ctgtggtaaa gccggtaaag gccagatagg gtctaacact
     8281 ccacaatccg tcaacccaac ggaacccgct ctcgacatga gcacccgcgc cgttactatt
     8341 gtaatcgcca aacgctgttg ccccattact catcttgcca tggatggtgt tggcaaaacg
     8401 gtcaactttc accactccat caacataggc accgttctga tgctcccaac cggcataagc
     8461 ccccagggta tagctatcga cattgccttt gccgccgcga tcaaaaccaa tatcagaatg
     8521 agagtaacca aagaacaagc cgcgaattgt gctgctttct tcacgggaga aacggctatc
     8581 gataccgagc gtcaggcccg tcaatgtttg ctcaaaacca gctcccgcat cagtggtcac
     8641 gttgttgcgg gtgttaattg ccgaactcca catcgccgta tcgtaactaa cgccttttac
     8701 gctaccaaga cgctcacgca cggtgtccag ttctgcatca aataccagcg gttgtgcggc
     8761 cgccatattc agcacatcag tggttgaagg ggtaatttgc gcgcgattct ctgccagact
     8821 ccagctatgg ttaccattat ccagcaaggt atattcatac gtaccgatat caacaacgcc
     8881 tccggcattg cccaacgtaa atgcagcatc accgccgccc gttgttacca gtgtaaggct
     8941 atctcctgct gccgggctgg caccggtgtc cgtcacgaat attttgaaat cacctgttgc
     9001 ctgaccggtg acgttgagct gatcgctctg atgattcgcc atatcggtac gcatataaaa
     9061 attgccgttt cccgacaggg tattggtggt caacgtagtg aaattttccg cacttgccgt
     9121 cacagatgaa cgagtcactg actcatccat cataatcaca tttgcgccag acatcgcaag
     9181 gttggctaag ttaaggcggg tgttttggtt cgtcgtagtg atatcggcaa tgatggtcca
     9241 tgcgctattt tcgagcgaaa catccgcttt gctggtatcc acggcaccat gcgttcttaa
     9301 agaagcgccc tcagaaacca ctaccttacc caccaacatt gcgggtgcat aatttatttg
     9361 cggcttcata acctcaagaa tgccatcatt tcctctgact gacacgttaa ccattgtgcc
     9421 agcccagacg ttagcgcggc cattgttgat aaccatattt tcagagatgg ctttagagga
     9481 atacgttata cttccattct gatctgatcg accgagatca tataccgcat ttgcctgcac
     9541 tctcgtacca gtatcctttc ccagcgattg catagtggca tgctcatcga ggatagtgtc
     9601 aatggcctgc gtgtcctcca taacaataag cccggaacca tcatccagtt cataattttt
     9661 tgacacacca tcttttatac taaattggcc tttactgttt gtaccactca cttccagcgc
     9721 attcgttgag acaattaatt ttccgcctgc ttttttatca acgccagttg cagtcccgcc
     9781 atccataacc tccagtaagc cgccactatc tacagtagta ttataagcga agtcattttc
     9841 ttcaactcgc aaacttccgc cgttttccaa cagcatattg ttagcaatac cattcttgat
     9901 ttcgaactga ccgagacggt ttgttccgaa tacctccatg acccgcgtgc ttgctttaat
     9961 agcaccgcct gctttctgat ctaccgcaat cgctaatccc ccctctttaa cctccagtaa
    10021 tcctcccttt tcgatagcgg caaggacggt aatcccatct ttttcaacga ttaagcgccc
    10081 gcctttaatc gtgggatcat tcgctgcgcc ataaacctgt agcacaccat tattgagcgt
    10141 agtctggttt gccgcgccac ccatttgaac aatttgataa ccatcattta atactgagta
    10201 ttctgcggtg ccattcattt caatgtactg atacccgccg ttaatttcag tattactact
    10261 tataccaaat tctttaatat gctgttctcc gccaggattg attttcgtat tattggcggt
    10321 cccataaacc agctggatat ctttttccag gacaacacca tcaactgtct caccatactc
    10381 aactgttgat gccaatgcta tgggtgaata taacgctgta gctaccagaa cagatattgc
    10441 ggaacgacaa agagaaacag aaccagattg atgcattgag ctttcatcct atgaaattaa
    10501 ttgcagttaa aagcattggg tacagaaaat acccatagct ccatacccgg agtcagtttt
    10561 taaaaactgt ttaaagaaat gcacaagtat tgtgattgat tttttagttg tttttcttga
    10621 tgagaagctg atgcaaaatt ccgtctttat aatgaaaatg atgccaaagc gaacgacaag
    10681 gttgtagttt tcgctacatg tccatacata aaatggggta acattcacgc gcctggtagc
    10741 gttaccaacg ctacgctcaa acataatgat tctaataaaa cctcaggaga ctactatgcc
    10801 tgaagcaacg ccttttcagg tgatgattgt ggatgatcat ccacttatgc gacgcggtgt
    10861 tcgtcagtta ctggagcttg atcctggctt tgaagtggtc gccgaagcgg gcgacggcgc
    10921 gagtgctatc gatctggcga atagactgga tatcgacgtg atcttgctgg atctcaatat
    10981 gaaaggtatg agtggcctgg ataccctcaa tgccttgcgc agggatggcg ttaccgcgca
    11041 aattattatc ctgaccgtat cagatgcctc cagcgatgtc tttgcactga tagacgcagg
    11101 cgcagacggt tatctgttga aagacagcga cccggaagtg ttgctggaag cgattcgtgc
    11161 cggagcgaaa ggcagcaaag tctttagcga acgcgtcaat cagtacttac atgaacgtga
    11221 aatgtttggc gcggaagaag atcccttcag cgtgctgacg gagcgcgagc tggatgttct
    11281 gcacgagctg gcacaggggc tgtcaaataa acagattgcc tcggtgttga atatttccga
    11341 gcagacagta aaagtacata ttcgcaatct actgcgcaaa ctcaatgtcc gctcacgcgt
    11401 ggcagccacc attctgttcc tgcaacaacg cggggcacaa taaaaatagc ccgatggcat
    11461 caccatcggg cctcttttta tttactctcc tgcggcgaca aatgttgcat cgcctgcgcg
    11521 atactacgtt caatcaccgc acggcgagta tcgttggcag gtaagagttt caacatcatc
    11581 tcccacgcgg caacggcttc gccaaatcgc tgctgctcaa aggcattaaa cgcatacatg
    11641 cttaacacac gaatattgct atggtccgtt ctcaccaact ggcgcagcag ttcgccgccg
    11701 aggcggttgt cgttgggatc agatgaacgt gtcaacgctt cagcataacc cagtgtggca
    11761 tcgctgtttt tcggatcgag gcgatacgca gtagcgtatg catcggtggc gatactggcg
    11821 ttacccagcg ccatgccaac gcgacccaac ataatccaac cttctatatc tcccggattt
    11881 ttttgcagtt gagtacgcat ccccagcgca aggcgcgaca tctcttcttc gttgagcgga
    11941 tcggctttcg gatccagcgc cctgtccagc aacgccggag cctgtgccgt ggcctgctgc
    12001 cagattttca cctgctgata attgccagtc tggtagtagc tgacgccagc cacaattaac
    12061 gccaccacaa tacccggcag ataaacaaca tatccggcac gcttaccttc cggcacgctt
    12121 tgttcaggaa acgcttccgg taccacgcgt acccgacgcc gcgaacgggc gtatatgacc
    12181 caaccgccaa taccaatagc cactaccgga agcacccaca gcagcacggt cagcggcgtt
    12241 aacggcggat cgtaagtgac gaagttgccg taacgcgcca ccatataatc gacaatctct
    12301 ttcttacttt tgccttcctg catcagttca tacactttct ggcgcaggtc ggtggcaatc
    12361 atcgagttgg aatcggcaat gctgttattc tggcatttcg ggcagcgcag ttcttcagtg
    12421 agctgacgga actgttgttc ctgtgcttca tctttaaact gcaacacgtc gatggtcgcc
    12481 agcgctgagc cggagatcat cagcatcagc acgcccaata aaaacctcat tgtgcggcct
    12541 ccttactgta tttctcccac agcggcttga tctcttcttc ccagacgcga ggattgagat
    12601 cgcccgcatg gcgatagcga atgatgccgt tgccgtcaat aagaaacgtt tctggcgcgc
    12661 catagacacc gagatccagc cctaacatgc catcgccatc aaaaaggctt agcgcgtaag
    12721 gattgcccag ctctttcagc cagctgattg ccttctggcg atcgtcttta tagttcatgc
    12781 cgaccacgcg gatgccctgc gcagaaagct gattcagata ttgatgttcc gcacggcagg
    12841 tcggacacca ggtcgcccag acgttaagca gcactggttt gccctgagtc agcacatccg
    12901 cctgataaaa ctgccccgga ttgtccagtg attcgagacg aaacttcggc acaggcttgc
    12961 caatgagcgc cgattccaga ttggtcggat catccccttc ggcattacgc gccagctgcc
    13021 acagcagcgc cgcggcaatc gccaggaaga taatcaacgg aattaacaat actttgcgct
    13081 tcatacagcc tccggcgcag ttttttgcgg gctcacgcgc ttacgatagc gaggatcaaa
    13141 cagacacagc agtccgccca acgccatcat cagcccgccc gcccaaatcc agcgaacaaa
    13201 tggtttgtag taaagacgca cagcccacgc gccgttttcc agctcttcac cgagcgcggc
    13261 gtacaggtca cgcgtgatgc cgccgtcaat tgccgcttcg gtcatcatcg acccggcagt
    13321 gttgtaataa cgtttttccg catacagcac cgtttccggt ttaccctctc gcgttacgcc
    13381 gatagtcgcc acaccgccac gccagttcgg gccagtcacc tctttgacat cacggaaggt
    13441 gaagcgatat tcatgaatat cgacgctatc gccggacttc atgcgcacat cacgctcaac
    13501 gctatagttc tggctaaagg caatgccaac aattgtcact gccagcccca ggtgagccgc
    13561 caccatcccc caataactga aggtggtttt cgtgccgcgt gaaatacgta gcgcagcttc
    13621 cgcaattgcc agcaccgcaa tccagcaggc cattgccagg ccgagcaccg tcatcgccac
    13681 aactttgctt tcgaacagcc acggcaacag cagagacagc accagcgtag agatgaaggc
    13741 gataatcaat aaattgcgga ttttacgtgg gcgatcgcgc ccccagcgca ccagaggacc
    13801 gacacccagc agtagcgcaa acggcaccat cagccaggta aacatggtgt tgaagaacgg
    13861 ttcgccaatc gaaatactgc ccagtcccag ttgcttgtgc accagcggca gcagcgtccc
    13921 cagcaacacc accagcatcg cggcgaccag caaaacgttg ttcgctaaca gcaaagattc
    13981 ccgcgaccac agcgcattgt ttacgcgtga gcgaactttg tgtccacgcg cggcaaacag
    14041 cagcagcgaa ccgccaatca ccagcaccat aaaggcgagg ataaacatac cgcgcgccgg
    14101 atcagacgca aacgcgtgta ccgataccag cacgccggaa cgcacgagga aagtccccaa
    14161 cagacacaac gagaaggcac tgattgccag cagtaatgtc cacgctttga agctggcgcg
    14221 ttgttcagtg accgccagtg agtgcatcag cgcagtcccc accagccacg gcataaacga
    14281 ggcgttttct accggatccc agaaccacca gccaccccag ccgagttcgt aataggccca
    14341 tgcggaaccg agcacgatgc cgagcgtcag gaagatccac gctgccagcg tccacggacg
    14401 agtaaaacgc gcataagtgc tgtccagacg cccgctcagc aaagaagcaa tggcaaaagc
    14461 aaacgccacc gagaaaccca cgtaccccat ataaagcaga ggcggatgga agatcagccc
    14521 cggatcctgt aacagcgggt taagatcgcg cccttcaatc gggaagttcg gcagcgtgcg
    14581 tgagaatggg ttagaggtaa agagaatgaa cagcaaaaag ccgacactga ccatccccat
    14641 tatcgccagt acgcgggcca caatatccag cggaatacgc tgactaaaaa tcgccaccgc
    14701 aaaggtccag ccgctcatca gcagcaccca cagcagcagt gagccttcat gtgcgcccca
    14761 ggtagccgcc acgcgatacc acaccggaag ctgggtattg gagttgctgg caacataggt
    14821 gacggtaaag tcgttgacca cgaaagcatt gaccagcacc agaaatgcgc cagccacaga
    14881 cataaacagc agccaggcaa acaagcggga agacgccatc atgcgcgcat ctccgcgcgc
    14941 cacgccccat agcggataca cggacagcag cagcgcaatt cccagcgcca ggcacagcag
    15001 cccgttacca atttccggca tcatgatgct gggtccttat aaacactcgc cgggcgacgg
    15061 tggttagctt ccatcgcttt ctcaacttct ggcggcgtgt agttttcatc atgtttcgcc
    15121 agcacttctt ttgcgaggat atgattgcct ttttccagct cgccctgcac cacaacgccc
    15181 tgcccttcac ggaacagatc cggcaaaatg ccttcgtaag agacatccac tgagccttca
    15241 gcgtcgtaaa tggtgaaggt cactttcagc gaattgggat cgcgctgcac actacccggc
    15301 atcaccatcc cgccaacgcg cagacgctga ccgacttccg gcatctgctg agtttcacgc
    15361 ttgccgtaga gaatttcccc cggcgtataa aagagatcga tattcgagcg cagcgcatat
    15421 agcaccagac cgatagtcag cgccagccct gccaacacgg cacaggcaat ccacaagcgg
    15481 tttttacggc gaatattcat gcagcctcct gctgttgcgc agcacgtaaa cgcgcctcac
    15541 gcgcccgctg ttgcgccaca ccacgcagaa ttgcgcgatg ctgcatcacc gagtgcacga
    15601 ccaaaaccac cagcggaata acggtcatca ccaccgccag ccagacaaaa aaggcgtaac
    15661 cgcccattgc gaaaaattca ttccaggaag caaatgcagg ggtcatttac ggcctctttt
    15721 cagtatcagt tcactcaccc acggacggcg tttttccatc agcaaaatca aattacgcat
    15781 ccgcatcagc gtcagcgtgg cagagaggag caggaagcca aaaatcgacc agcgcagcgg
    15841 cgaacgcatc gccggatcga tactttgctg catccgcgtt gatccctgat gcagggtgtt
    15901 ccaccactcc acggagtaat gaataatcgg cagattcacc acgccaatca gcaccaggat
    15961 acctgccgca cggcccgcca gacggcggtc gtcgaaggcg tgccacaggg caatcacacc
    16021 cacatacaaa aacagcagca ccagttcaga agtcagacgt gcatcccata cccaccaggt
    16081 gccccacatc ggttttcccc atgcagagcc ggtaaccagg gcaataaagg taaacacggc
    16141 accaatgggg gccatcgccg ccaccgccag gttggccatt ttcatctgcc agacaaggcc
    16201 aataaacgcc gccaccgcca ttgatgcata aatgcccatc gaccagatcg ccgcaggcac
    16261 atgcaggtag ataatgcggt agctatttcc ctgctgataa tcagccggag caaagccgaa
    16321 tccccagatc cagccgacgg taagcacgac tacactggca attgccagcc acggtataaa
    16381 ccagccacag atttgataca gccgtggtgg gatcgccagt tgatgcagtg ttttccacat
    16441 agtttcgata ccagactcga acaaaaatca gtaatccagc gttattgaat gctgattcgt
    16501 aacgctgccg ccgtcgcaaa aggacttaat gtcgcggtgc ctgccagcaa cgcgcctaaa
    16561 attgccagat acccgtcaac gggcaaatgc atagaagccg cgtccatcgc ggcggtggca
    16621 aagatgagta atgggatagt cagcggtaac accagtatgc tgagcagcac accaccgcgc
    16681 ttaagtccca ctgtcagcgc cacgcccggt gcaccgagaa agccaagcgt aggcgttccc
    16741 agcagtagcg ttaacgccat cacttgccag ccataaacat ccattcccag tagcattgct
    16801 accagtggcg aaaggatgag taacggcaga ccggttacca tccagtgcgc catcaccttc
    16861 gccagcacaa cggcgggcaa gggtaacggc aacaacatca attgttcaag actgccgtcc
    16921 tgcaaatcgt cacggaacag tcgttccagc gccagcaagg atgaaagcag cgcggcaacc
    16981 cagataatgc ccggtgcaat acgtgccagc agttgcggct ccggaccgat actgagcgga
    17041 aaaagggtaa ttacaatcag gaagaaccac agcgggttgg cgatttcagc gctatggcga
    17101 aacgctacgc gcagctcaag acggaaaatg cgccagaaca tcatacggcc ctcgtttgcg
    17161 tcagtgaaat gcggcgaatt ttactttcag caacgttgag cggctggtgg gtagtgagaa
    17221 tcacaatccc cccctgctct gtatgctgcg ccatacgctg ggtcagacga tcgacgccgt
    17281 taacgtcaat cgcggtaaaa ggctcgtcga ggatccataa cgtggcacgg gtcagccaca
    17341 gacgcgctaa agcgacgcgg cgttgttgcc cggcagaaag ttgatttacc ggaatatctt
    17401 cgaatccggc aagcccggcc tgcgccaggg cttccagaca ttgtgcggta tcgccatcgc
    17461 gatgataaaa gtgcagattt tctaacgccg tcagccgggt tttgatcccc ggctgatggc
    17521 ctatccataa caggttttga tggtagctgt cgcgtacctg atgcaagggc tgcccttgcc
    17581 agagaacctc gcctgcgtca gggcgagaca accccgtcag caaacggaga agcgttgtct
    17641 tccccgcgcc gttgctaccg gtgatttgta cccactctcc tgcgttcagc gtaaatgaca
    17701 agccactaaa taaggttcgt tcatcccgct cacaaagtaa ctctctgact tcaagcatac
    17761 ccacgcaata accctgttaa aaacctggct cgacttcacg catatcgggc agcttgtgcg
    17821 ctatcccttt atggcaatca atacaggttt gcccatcttt caccgcctgg tcatgcatct
    17881 tcgcggcaac cgatttctgg gcggttgtat ccatatactc gaagttgtga cagttacggc
    17941 actcctgcga gttattgtcc ttcatgcgcc gccactcatt ctgtgccatc gtcagacgat
    18001 gagcttcaaa tttctgcggc gtgtcaataa cgccaaaaat tttaccatac agctctttac
    18061 ttgctttgag cttgcgtatc atcttcggca caaactcgtg cggaacgtga caatccggac
    18121 aggtcgcacg gacgccgcta cggttgttgt agtgcacgga atccatgtat tcctgataca
    18181 ccgtgttacg catttcgtgg cagctaatgc agaactcttc ggtattggct ttttccatcc
    18241 cagtgttaaa gccgccccag aagacgatgc cgccgacaaa accgatcaac agcagcgtcc
    18301 ccagcgccag acggctgggg gtacgccacc atttccacag gcgcttaatc agaccaggct
    18361 tacggtcaga atttcccata ataacctctt atttcccgta accttttgat ggggtaaagg
    18421 tattccccac gattggcgcg gtatcggcct gcggtacgtg acattgcaga cagaaataac
    18481 gacgcggagc cacttccgcg cccactttgc cgtcgctgtc cataaagtga gtaggactga
    18541 tacgcggcgc gccagtggtg cgatagcttt cgacaccgtg gcactgcaag cagcggttgg
    18601 tattggtcgt tacctgataa ccttcaacgc tatgcgggat cattggcggc tgattcacat
    18661 agttcagcgg catccgatcc tgctcttttg gcatccgaat ggccccttcc tgcgtcccgg
    18721 atacttccgg cgactggcta aaatcgacgc cattagccgc ccaaaccgcg ccgcttacca
    18781 ccagggccag catcgccgtc cattgacaca gcgctttctt caggtcatgg cttttcatga
    18841 tttcgctccc gaactccatc gtgtagttat tgtaaataca tcctcagaac agacatccac
    18901 gcagcgaccg cacgtcatgc aatcgcggct ggtgacctgc accgggcttt gttcatccag
    18961 caccggggca cgtagcacat gcggttccgg gcaaacatga aaacaatcca tacagcggtt
    19021 acatttctgc cgatcgctgg ccgcaacggt aatcacacct ttgctaccca gcacgccata
    19081 cagcgcgcca accggacaaa tgtgcccgca ccagccgtgt tcaacgacca gtagatcaaa
    19141 taaaaacagc gcgagaataa gcagcgcgcc gctgccgaag cccattacca ggctacgccc
    19201 catcagggaa acggggttga tccattccca gatgagcgtg ccagttaagg ctgatcccac
    19261 cagaatgacc accaacagca cgtagcgaat gtggcgaggg atcgtcgcag actgattgag
    19321 gtcaaagcgc ctgcgtaacc agttcgctaa gtcggtaatc gggttcagcg ggcagaccca
    19381 gctgcaaaat aatcgctttc ctgccagggc gtagagcacg gtgataatca ccgcaccggt
    19441 caacgccacc gtggcaggca gatgaccact ggcaagactt tgcagcgtca tcagcggatc
    19501 ggtcagcgga acggtgtcga ataacaggct actgctatag ttgccgtgca agatccacac
    19561 accaaaccac ggaccgctca aaaacatccc cagcacgaag aactgacaaa ggcgacgtaa
    19621 caccagccaa cggtgactgc gccaccagcc ttttttctcc agcgcctcgc gcccggcgtc
    19681 acgtttacga tttgccattg ttcccctcca gccagccgaa gcggtaatgg tgacctaact
    19741 cccctttcgc cagtgacagc ggtaacacct tgattgccgg ttgttccagc acgcatactt
    19801 tttcgcactt accgcaaccg gtacaggcgt cgctgtgaac cgtcggcaga aagcgggcgt
    19861 gcttaccggt acgcgtgttg cgctccagct ccagggtgat ggcctcatca attttcgggc
    19921 attcgcgata acaaacatcg cagcgcagcc cctgaaagtt gagacagttt tcctggtcca
    19981 ccagtaccac cagccccatc cgcgcgtcgt cgatcgattc aatctcacga tccagcgcac
    20041 cgctcgggca cactttggcg cacggaatgt cctcacacat ttcgcaagga atatcccgtg
    20101 cgacaaaata tggcgtgccc gccgacaggc cagaggccag cgtcgccagt tttaaggtgt
    20161 cgtaagggca agcctgaaca cactgaccgc aacgcacaca ggcactggca aaggcgttct
    20221 cgtttatggc tccgggcggg cgcaaccgca cgccagatgc gcgtgcggtt tgctgttgta
    20281 accccagcgc cacacccacg gcagccagcc cgcctgctgt gcgaacgaca tcgcgcagaa
    20341 agcggcggcg accattttga ggtttcgctg accgggacat aatggcttac accttctcca
    20401 gtttgaccgc gcacttcttg aagtccgtct ctttcgagag cggatcggtc gcgtccagcg
    20461 ttagtttgtt aaccagctgt gcggcgtcga agaacggcat gtacaccagg ccctgcggcg
    20521 gacggttacg accgcgcgtt tcgacaatcg agatcacttc gccacggcga gaaaccactt
    20581 tcactttgtc gccacggcgc agatcgcgcg ctttcgcatc cagcgggtga ataaacagga
    20641 ccgcttccgg gaaggcacgg tgcagttccg gcacacggcg agtcatactg ccggtgtgcc
    20701 agtgctccag gacgcgtccg gtagagagcc acaggtcgta ctcttcatcc ggtgcttccg
    20761 ccgccggttc gaacggcagc gcgaagatca ccgctttacc atccggttta ccgtagaact
    20821 tatagccttc gcccgctttc acgtacgggt cgttaccttc gctgtaacgc cactgcgttt
    20881 ctttaccgtt caccaccggc cagcgcagac cgcgcgcttt gtggtagtca tcgaacggcg
    20941 cgagatcgtg accgtgaccg cgaccaaacc atgcgtactc ttcgaacagc cctttttgca
    21001 gatagaagcc cagctcgcgg gattcatcgt tcagctgatc ttccgccagt tcggataccg
    21061 ggaatttgct cacttcgggg gtggcataca gaacttcgta cagcgttttg ccacgcagtt
    21121 ccggtttctt cgccagcagc tcttccggcc atacttcttc agttttgaag cggcgggaga
    21181 actggactaa ctgccagagg tccgatttcg cttcgcctgg cgcctgtacc tgctgacgcc
    21241 agaactgagt acggcgttcg gcgttaccgt aagccccctc tttttctacc cacattgcgg
    21301 tcggcaggat caagtcggcg gccagcgcac tgactgtcgg atacggatcg gagacgatga
    21361 tgaagttgcg cggatcgcgc cagcccggca tacgctcttc attaatgttc ggcccggcct
    21421 gcatgttgtt ggtacacatg gtccagtaaa cattcagctt gccgtctttc agcgcacggt
    21481 cttgtgctac cgcatgcaga ccgattttcg ccggaatggt gccgctcggg atattccact
    21541 tcttctcgca gatatcacga tgtttctcgt tagttaccac catgtccgca ggcagacggt
    21601 gagcaaaggt gcccacttca cgcgcagtac cacacgcgga aggctgcccg gtcagggaga
    21661 acggaccgca acccggctgg gaaattttgc cggtcagcag gtgcaggttg tagaccaggt
    21721 tgttggccca cacgccacga gtatgctggt tgaagcccat cgtccagtag gagatgactt
    21781 tcttgttcgg atcggcatac agctgcgcca gttgttccag ctgatctttt ggtacgccag
    21841 tcatttcagc ggttttttcc agcgtatact cagcaacgaa ggctttgtaa tcttcaaagc
    21901 tcatcggttc ggaggcgtca gaacccggat tcttcgctgc tttttccagc ggatgggtcg
    21961 gacgtaaacc gtagccgatg tccgtcgccc ctttgcgcag gttaacgtgc ttgctgaaga
    22021 agtcctgatt tatcgcattg ttttgaatga tatagttggc gatgtagttc aggatcacca
    22081 ggtcagattg cggcgtaaag atgatgccgt tatccgccag ctcgaagcta cgatgctggt
    22141 aggttgaaag caccgccacg gtgacgtcct ggttagagag acgacggtta gtgatgcgtg
    22201 accagaggat cgggtgcatc tccgccatgt ttgcgcccca cagcacaaac gcgtcagcct
    22261 gctcgatgtc gtcatagcag cccatcggct catccatacc aaaggttcgc ataaagccaa
    22321 ctactgccga cgccatacag tgacgcgcat tcgggtcgat gttgttcgaa cggaagcccg
    22381 ctttaaacag cttggacgcg gcataacctt cccagatagt ccactgacca gaaccgaaca
    22441 taccgatcga ttccggccct ttttctttca gggcggtttt gaatttctct tccatcacat
    22501 cgaaggcctg atcccaggtg attggggtaa attcgccttc tttgtcatat ttaccgtttt
    22561 tcatacgcag cagcggctgc gtcaaacggt ctttaccgta catgatcttg ggcaggaaat
    22621 agcccttaat gcagttcagg ccacggttaa ccggtgcgtc cgggtcgccc tgacaggcca
    22681 ccacacgccc ctgctgcgtt ccgaccagaa cgccgcaacc agtaccgcag aaacggcacg
    22741 gcgctttatc ccatttaatg gcttcctgct gaccaacaac ggcgcgggca acgcccggca
    22801 cgctgagacc ggcagccgcc gcagcggccg caacggcgtt agctttcata aagctacgac
    22861 gactgagttt catggtgttt cctcaccttg ctcttcctgc tggtgataaa ccagcgacac
    22921 cgccagcacg ccctctacgt tgcgtactga ctcaatggtt tggatcagcg tttcgctgtc
    22981 ttctgcttcc accaccacaa tcaactgacc gctcggcgcg tcgctgacag caacttcaca
    23041 gccgggaaag gcgttcagtt gggtgctgat gtctgaaatt cgttcgcttt tggcctgcac
    23101 aaccaggctg caaacttgcc agttagtgtg catggagata ctccgcagtt atggctgata
    23161 ccggacagct ggctgcacat gctccacatc cgttgcaaag ttggctatta agttgcggct
    23221 ggtagatccc ggagagcgtc gggcgaaaga taattgccat tggctcacag ctatcctgac
    23281 agcggcggca ttcaactgac tgatacgcca ggcacgcgtc cccaatggtg aactgcaaat
    23341 cccaggccct ggtgtggcgc ggagaaaata gcgattcggg gcaagcctgc gcgcaggcgt
    23401 aacagaagct gcactcgtta tttttgaaat taacgctcgg atagccgccc gcgccgcgtt
    23461 gcagaatgtt gttttcacaa gcattaatac aggcgtcgca gcgggtgcaa tgggtcagaa
    23521 aatgagattc atcaccggac cagggcggac ggataccgtt actggctttg cgccagcgac
    23581 cagtgagtat gccccgacgg gatgcatcaa tcttcacatt gaccttccat cattaacgcg
    23641 ctctgaaaat tgagagcgac caaataaacc gcataattaa taagccattt ttatagccgc
    23701 taagatatta aaggatgtgt caaagatgca taccccgatc gaggtaaaac ctgtagcagg
    23761 atcaaaagag tggcgggaag cgt
//
LOCUS       DBFIFI010000058        23717 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.058, whole genome shotgun sequence.
ACCESSION   DBFIFI010000058 DBFIFI010000000
VERSION     DBFIFI010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23717
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.058"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            242..2914
                     /gene="rcsD"
                     /locus_tag="N7B39_002156"
     CDS             242..2914
                     /gene="rcsD"
                     /locus_tag="N7B39_002156"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HGU2422138.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            2931..3581
                     /gene="rcsB"
                     /locus_tag="N7B39_002157"
     CDS             2931..3581
                     /gene="rcsB"
                     /locus_tag="N7B39_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HGU2422139.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(3781..6630)
                     /gene="rcsC"
                     /locus_tag="N7B39_002158"
     CDS             complement(3781..6630)
                     /gene="rcsC"
                     /locus_tag="N7B39_002158"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HGU2422140.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKADPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLMIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(6905..7681)
                     /gene="yfaP"
                     /locus_tag="N7B39_002159"
     CDS             complement(6905..7681)
                     /gene="yfaP"
                     /locus_tag="N7B39_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HGU2422141.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDSSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(7686..9335)
                     /gene="yfaQ"
                     /locus_tag="N7B39_002160"
                     /pseudo
     CDS             complement(7686..9335)
                     /gene="yfaQ"
                     /locus_tag="N7B39_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
     gene            complement(9336..13850)
                     /locus_tag="N7B39_002161"
     CDS             complement(9336..13850)
                     /locus_tag="N7B39_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HGU2422142.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEESKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELQLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGKELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVDVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     NARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGDLPSGGKSFTVNFDKPGNYNLTLRDKDGLILAGLSHAVSGKGSM
                     SHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDKPSAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLAQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGDNALMLPEQASNIRLQSSETPQEIFRNNLD
                     ALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKTEDFSEEDTRDINDSLILDTPESPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAVNSNQPLAHTVVLLNSGGDATQAAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPSVVLPAPAGAWAKHKLTGGGEYWRWVGQGVP
                     DILSFGDELSPQNVQVRWREAAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAGKQQGQL
                     LEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            complement(13874..14497)
                     /gene="yfaT"
                     /locus_tag="N7B39_002162"
     CDS             complement(13874..14497)
                     /gene="yfaT"
                     /locus_tag="N7B39_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HGU2422143.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(14494..16122)
                     /gene="yfaA"
                     /locus_tag="N7B39_002163"
     CDS             complement(14494..16122)
                     /gene="yfaA"
                     /locus_tag="N7B39_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HGU2422144.1"
                     /translation="MALLSAGVWALQYAGSGPEKTLSPLVVHNNLQIDLNEPDLFLDS
                     DSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIEGSIRRIVYEHDLTLKDKLFS
                     SLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPLLFAATSDSQLSKTEISSIKI
                     NSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFKDDQQDTEATAIASDLLSGKK
                     RWQASFGLEERAAEKTPVRQRIVASARLLGFGYQRLMPSFAGVRFEMGNDGWHSFVAL
                     NDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAEEMLSHISQENDKLNGALDGA
                     AGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNIGAHESKAPEGVLPVSQTQQG
                     EAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLAMQNKTLLFSLDDTLVNNALQ
                     TLNKTRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLTSLPKNLEPVFYNAAQTLLMP
                     KLDALSQQPRYVMKLAQMEPGAAWQWLPIIWQPL"
     gene            complement(16330..18957)
                     /gene="gyrA"
                     /locus_tag="N7B39_002164"
     CDS             complement(16330..18957)
                     /gene="gyrA"
                     /locus_tag="N7B39_002164"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HGU2422145.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            19104..19826
                     /gene="ubiG"
                     /locus_tag="N7B39_002165"
     CDS             19104..19826
                     /gene="ubiG"
                     /locus_tag="N7B39_002165"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HGU2422146.1"
                     /translation="MNAEKSPVNHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LSYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNTFKLGPGVDVNYMLHTQNK"
     gene            complement(19967..>23717)
                     /gene="yfaL"
                     /locus_tag="N7B39_002166"
     CDS             complement(19967..>23717)
                     /gene="yfaL"
                     /locus_tag="N7B39_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HGU2422147.1"
                     /translation="RVIFLRKEYLSLLPSMIASLFPANGVAAAIDLCQGYNIKASCHA
                     SRQSLSGITQVWSIADGQWLVFSDMTNNASGGAVFLQQGAEFTLSPENETGMTLFANN
                     IVSGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAIDLRVTNAV
                     FRNNIANDGKGGAIYTINNDIYLSDDVFNNNQAYTSTSYSDGDGGAIDVTDNNSDSKH
                     PSGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSAA
                     GYGDGPSSAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSGD
                     LVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQNQ
                     AELNVGSTQQTFAHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSYV
                     LAGAQSMALTGDIVVDAGAVLSLEGDAADLAALQDDPQSIVLNGGMLDLSDFSTWQSG
                     TSYKDGLEVSGSSGTVIGSQDVVDLAGGNDMHIGGDGKDGVYVVIDAGDGQVSLANDN
                     QYLGTTQIASGTLMVSDNSQLGYTHYNRQVIFTDKPQESVMEITANVDTRSTTTEHGR
                     DIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTLTKTGAGTLELTASGTTQSAV
                     RVEEGTLQGDVADIFPYASSLWVGDGATFVTGADQDIQSIDATSSGTIDISDGTVLRL
                     TGQDTSVALNASLFNCDGTLVNATDGVTLTGELNTNLETDSLTYLSNVTVNGNLTNTS
                     GAVSLQNGVAGDTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVMNGNTAGNTTVVVNS
                     ITGIGEPTSTGIKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRS
                     QEVTPPSPPDPDPTPDPDPTQDPDPTPDPEPTPAYQPVLNAKVGGYLNNLRAANQAFM
                     MERRDHAGGDGQTLNLRVIGGDYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGTDGEWM
                     LGIVGGYSDNQGDSRSSMTGTRADNQNHGYAVGLTSSWFQHGKQKQGAWLDNWLQYAW
                     FSNDVSEHEDGVDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAAN
                     RARVSQSQGDDIQTRLGLHSEWRTAVHVIPTLDLNYYHDPHSTEIEEDASTISDDAVK
                     QRGEIKVGVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
BASE COUNT         5673 a         6472 c         5814 g         5758 t
ORIGIN      
        1 caaataatta acatcagcat aatttccagc aatctttgtt tatttgcaat tattttcgtt
       61 gggctttttg taggttattt gtacagcaaa atggcgcttg tacatctatt tcccccaatg
      121 caggatgata aatatcacgg gagaatagag aatcatcaat caggtaagag tctggaattt
      181 cacactgtac cctttatact gccctatcac ttcgcgaagt tttaacaggt cataaacacg
      241 aatgcgtcag aaagagacaa cggccacgac ccgcttttca ctcctaccgg ggagcattac
      301 ccgcttcttt ttactgttga tcattgtgtt actggtgacg atgggtgtaa tggtacaaag
      361 cgccgttaac gcctggctga aagataaaag ttaccaaatt gtcgacatta cccacgctat
      421 ccaaaagcgc gtcgatacct ggcgttacgt gacctggcag atctacgaca acattgccgc
      481 gacgacctcc ccctcctccg gcgaaggttt acaagagacg cgcctgaaac aggatgtcta
      541 ctatctggaa aaaccgcgcc gcaaaacgga agcgttaatc tttggctctc acgacaactc
      601 aacgcttgag atgactcaac ggatgtccac ctatctggac acattgtggg gcgcagaaaa
      661 tgtaccgtgg tcaatgtatt acctgaatgg tcaggataac agtctggtgc tgatctcaac
      721 cctgcccctc aaagatctca cctccggatt taaagaatcg accgtcagtg acattgttga
      781 ttcacgtcgt gcagagatgt tgcaacaggc caacgccctc gatgaacgcg aaagtttttc
      841 taacatgcgc cgcctggcct ggcagaacgg tcattacttt accttgcgta ccacgttcaa
      901 ccagccggga catctggcaa cggtcgtggc ttttgatctg ccgattaatg atttgatccc
      961 accgggtatg ccgctggaca gtttccgcct tgagccagac gcgacggcaa cgggaaacaa
     1021 tgataatgag aaagaaggaa cggatagcgt cagtatccac tttaacagta cgaagattga
     1081 aatctcctcg gcactcaact ctaccgatat gcgcctggtc tggcaggttc cttatggcac
     1141 cttattgctg gatacgttgc aaaacattct gctgccactg ctgctgaaca tcggtttgct
     1201 ggcgctggcg ttatttggct ataccacatt ccgccatttc tccagtcgca gtacagaaag
     1261 tctacccaac acggcggtca ataacgaatt gcgcatttta cgggcaatca atgaagagat
     1321 agtctcactg ctgccgctcg gcctgctggt tcacgatcag gaatcgaacc gcactgtcat
     1381 aagtaacaaa attgccgatc atttgctgcc gcatttgaat ctgcaaaaca tcaccaccat
     1441 ggcggaacag catcagggga ttattcaggc gacgatcaat aacgagctgt atgagatccg
     1501 catgttccgc agccaggttg cgccgcgcac acaaattttc attattcgcg atcaggatcg
     1561 cgaagtgctg gtaaacaaga aactcaagca ggcgcagcgt ctgtatgaga aaaaccagca
     1621 ggggcggatg acctttatga aaaacattgg cgatgcgctg aaagaacccg cacagtccct
     1681 ggcggagagc gcggctaaac tcaacgcccc ggaaagcaaa caactggcga atcaggcaga
     1741 tgtgctggtg cgattggtcg atgaaataca gttagcgaac atgcttgcgg acgatagctg
     1801 gaaaagtgag acggtgctgt tctccgtgca ggatttaatt gatgaagttg tgccttcagt
     1861 gttgcctgcc atcaagcgta aaggtctgca actgctgatt aacaatcatc tgaaagcaca
     1921 cgatatgcgc cgcggcgatc gcgatgcatt acgacgtatt ttgctgctac tgatgcaata
     1981 tgccgtgacc tcaacgcaat tgggaaaaat cacccttgag gttgatcagg atgagtcctc
     2041 cgaagaccgc ctgacgttcc gcattctgga caccggagaa ggcgtaagca ttcatgaaat
     2101 ggataatttg cacttcccgt ttatcaacca gacccaaaac gatcgctatg gcaaggcgga
     2161 cccgctggca ttctggctga gcgatcaact ggcacgtaaa ctgggcggtc atttaaacat
     2221 caaaacgcgg gatgggcttg gtacacgcta ctctgtgcat atcaaaatgc tcgcagctga
     2281 cccggaagtt gaagaggaag aagagcgttt actggatgat gtctgcgtaa tggtggatgt
     2341 tacttcggca gaaattcgga atattgtcac tcgccagtta gaaaattggg gtgcaacctg
     2401 tatcacaccc gatgaaagat taattagtca agattatgat atctttttaa cggataatcc
     2461 gtctaatctt actgcctctg gcttgctttt aagcgatgat gagtctggcg tacgggaaat
     2521 tgggcctggt caattgtgcg tcaacttcaa tatgagcaac gctatgcagg aagcggtctt
     2581 acaattaatt gaagtgcaac tggcgcagga agaggtgaca gaatcgcctc tgggcggaga
     2641 tgaaaatgcg caactccatg ccagcggcta ttatgcgctc tttgtagaca cagtaccgga
     2701 tgatgttaag aggctgtata ctgaagcagc aaccagtgac tttgctgcgt tagcccaaac
     2761 ggctcatcgt cttaaaggcg tatttgccat gctaaatctg gtacccggca agcagttatg
     2821 tgaaacgctg gaacatctga ttcgtgagaa ggatgttcca ggaatagaaa aatacatcag
     2881 cgacattgac agttatgtca agagcttgct gtagcaaggt agcctattac atgaacaata
     2941 tgaacgtaat tattgccgat gaccatccga tagtcttgtt cggtattcgc aaatcacttg
     3001 agcaaattga gtgggtgaat gttgtcggcg aatttgaaga ctctacagca ctgatcaaca
     3061 acctgccgaa actggatgcg catgtgttga ttaccgatct ctccatgcct ggcgataagt
     3121 acggcgatgg cattacctta attaagtaca tcaagcgcca tttcccaagc ctgtcgatta
     3181 ttgttctgac catgaacaac aacccggcga ttcttagtgc ggtattggat ctggatatcg
     3241 aagggatcgt gctgaaacaa ggtgcaccga ccgatctgcc gaaagccctc gcagcgctgc
     3301 aaaaaggaaa gaaatttacc ccggaaagcg tttctcgcct gttggaaaaa atcagtgctg
     3361 gaggttacgg tgacaaacgt ctctcgccaa aagagagtga agttctgcgc ctgtttgcgg
     3421 aaggcttcct ggtgaccgag atcgctaaaa agctgaaccg cagtattaaa accatcagta
     3481 gccagaagaa atctgcgatg atgaagctgg gtgtcgagaa cgatatcgcc ctgctgaatt
     3541 atctctcttc agtaacgtta agtccggcag ataaagacta atcatctgta ggccagataa
     3601 gacgcgttag tgtcttatct ggcatttgca ccgattgccg gatgcggcgt aaacgcctta
     3661 tccgtcctac gattcccatt atttcaacag attacattaa agtaggccag ataagacgcg
     3721 tcagcgtcgc atctggcatt tgcactgaat gccagatgcg gcgtaaacgc cttatccgtc
     3781 ctacgattcc cgcgatttcc tgaccctctc ggcatatacc gtcagcgtct gttttataac
     3841 atccagcgtt actggcttcg acaggcagct gtccataccg gactccagac accgctgctt
     3901 ctcttcagcc aacgcattag ccgttactcc gattaccggc aacgtcagtc ccaactgacg
     3961 aatgcgttgt gtcaagcggt aaccatccat atttggcatg ttgacgtcgc taagcacgat
     4021 atcaatatga ttcttgctaa gtacattaag cgcatcgacg ccatcattcg cggttttaca
     4081 ttgatagccc aacgatccca actgatctgc cagcaaacgc cggttaatcg gatgatcatc
     4141 cacgaccaga atcatcatat cgtcattatc gctgaccgct ttgtccgtcg acggcagagc
     4201 gttagcagga tcgtcgctct ccatctcgat caaataaata cgcgccaaca atgccggtag
     4261 ctcatgcgga gcagccacac tgtgtaccca ctcccctggc gctttctcca gcggaatacc
     4321 aatatgtcga cgacagaagg tcactaccgc tctgccctgc cattttttac tcactacctc
     4381 gtcagtgatt aacacatctt cgggagtcgg ttcctgccct tcgtatgttg ttacgacgat
     4441 gccgctgcgc tgcaaactgg tttccaggaa ctgacagagc gacgcattgc ggaccgccag
     4501 ccagcagcgt ttaccactca acccttccac gccttttttc tgcgggtact gagcgccgta
     4561 caacggaata cgcacggtaa actggctgcc cattcccggt tctgaatcta ccgagatatc
     4621 gccgtccatc atgctgatca gtttttcaca aatcgccaga cccagaccgg tcccctggaa
     4681 gtttcgctgt acgcccgttc cgacctggaa gaagggatca aacaagcgca ccacttcttt
     4741 cgccggaatc cccacgccgg tatcgcgaac acggatagag agataatcgc catcagcgcg
     4801 gacatgcaaa actatacagc cggtatcggt gaattttatg gcgttactca acaggttgga
     4861 gatgacctgc tgtaaacgca tcgggtcgcc atttaaggcc actggcacat ccggttcaat
     4921 aaagcagtac aagcctaact gcttgcgtac caccagcggt aaatagttgg cggtgatgtg
     4981 gttcatcact tcacgcggtg aaaactcacg cggttcgatc atcaactgtt ccgattcaat
     5041 cttcgagaaa tcgagaatat cgctgataat tttcaacaac aggctggaag agttgttcat
     5101 tgccgtcacc agacgatcga cgcctttcgg taactctttg gtttgcaaca gatccaggtt
     5161 accgataatg ccatacagcg gcgttcgcag ctcatgactg acggtggcaa ggaacatcga
     5221 ttttgactgg ctcgcctgtt ccgctgcttg tgccatctcc tgcaacgact cttccatctt
     5281 cacgcgcgaa gaaacatcca ccagcacaca aatggccacg ttttcattac gatagcgcga
     5341 atggacgaag ctgatttgca gattggtatt gttgctggtc aggacatcaa caaaattgac
     5401 ctgctgccca cagataattt gtgtcagtcg ttggcggtcc tcatgcgtaa gcatattgag
     5461 ataggtatgc gccagttcgt tacttaaaat attgacgcca tcagcggtac gcaaaatgca
     5521 gatacccact ggcgcggagg cgacaatctt gcgattgaac tgctcatgtt cttccagtcg
     5581 cagggcgtcg ctttccgccg gaatgaaaat acgtcgctcg tacatccgcg cgagagtaaa
     5641 caatgcagct ccggcaagca cattcagcaa aattgcgtta aggatcaaca tgcgaatgcg
     5701 ttccagcacc ttatcaaccg gcaccgaata cacgatgctg agcgatgagg gtggcagatt
     5761 tttcttcagc accagctccc ggaacccttc cgtatagcca aaccaggagc gttcctgcat
     5821 ccagcgagga tcggccttaa ttttgctttc tggcccggta agcgaaatca gggtatgacc
     5881 attttcatca agaatggtaa cccccatcgg caacgtaccc ggtaagaaaa agttctccat
     5941 ccggatggtc tgctcgacac ccaaaagcgc ctgcaaccgg ttcgccagat aaactggcgt
     6001 caacgcgtaa aaatacccga cgccagggcg cggaccttcg ctgatccagt agaggttact
     6061 gccgctatca tcttgtggtg catttcgata tttattgatg cgttcatgca aagctttcaa
     6121 cgcggtatcg cgttccactg gcatatcacg cagaccgaaa ttggccatgc agaggttatc
     6181 gctgccgatt aaaaataccc ggttgagatc gtaagccgca gaaaaattat cgcgccagta
     6241 gcgcataaac cacgccaatg actccagaga acctcgccag gtgttactca ttgctgaaca
     6301 atcggagtcg gcaaacagcg gttcaaacgc aggcacatcc gcctgcgttt ctcgtccacg
     6361 cggggaaagc acaccgtttt ctgccgataa gcgattttcg gcgatgtact tcagctcttt
     6421 catcacatca gaggtgcgtt gaataaagcg ctgagcctga tcggaactca gattaaattc
     6481 ctgacgaatt tccgattctc gctgatgtaa cgcattaacg atgtaaaaaa cggatgaaaa
     6541 agcaatcaac agccagagca ctaacgccaa tgctctgaac atgtagcgcg aggctttcag
     6601 ggttgtacga aaagaagcaa ggtatttcaa aggggcgaag ctccgcctca ggtgaccgat
     6661 ggagtgtggt taaggtagcg gtaaaagcgt gttaccgcaa tgttctgtca ccattgcaat
     6721 aatgcgtatc gcttagaaac cattgcctta accaggaaag ccggagtaaa tggacagttc
     6781 tcagtacgaa aaataagaaa tgaccaatct gccgacgctt gttaatggtt atatcctggc
     6841 gtcggcagat cgcccagcag tcttatctga aaaaagaaag agccaggttg tgtgaaggga
     6901 tggatcacca gtcaaaactt ttcaccagcg tcagctcgcc agcattacgc atcggtacaa
     6961 taaatgtttc ctgtttctca ttgaccgatc cttcatcggt gatcagcgtc agttgggcgg
     7021 tggttaattc cgtttcgctg cgcccaccat agtagttgat atacacctga tagcgcccgt
     7081 gaactggtgc tggcatggcg aaaatctcgg gtccgtaccc cgtcgtgaca tccatatcca
     7141 atgcaccact gtttttcagc acggtgttac cgtaccaggc gtgttcgcca tcgggcgtga
     7201 caacgtgaag atcgaggtcg gtattgtccg tatcccacga gagaaccagc cgtaaacgtg
     7261 cacgaatcgt tcctgtaccc ggcgttgagt aaaactgcat tttttgtcgg ctttgcccat
     7321 ccgggctgat gacctgcacg ctgttgctgc cttcagtgaa aatataaggg cgtgcaaatg
     7381 aactatcgga ttcaattctc tgcggcatac tggcaccgtt gaccaccagc cggccctgct
     7441 gaactttacc cgccgcagca taattcttta ttttgccgcg gatctgcgct gagatatttt
     7501 gatcatctgc catgttgacg gacgacgccg ggtaattgat cgattgtgaa aaactggcat
     7561 cttcgccctc tgccggatgc cagccagaga gcggtgcgtc aatctcgact tcctgcacac
     7621 cttcactgtg agcgacaggc gaaagcgcca ccagtaacaa cggaagaaaa atctttcgca
     7681 tagcattagt ccattaataa ttgtcgggtc agcgtttcga tatagttctc atcaagcccg
     7741 gtgggataac catcaaaggc caggtgcaga tactcatggg ttaaatccag ccgatcctga
     7801 agcgtaaaga agttacgaat aaacagccgt ttctgctgcc gatcggtata ggggaaacct
     7861 gagactaaac gacagaccgc aaacacgtct ggttcgttgt accccgtctc gccttgtaat
     7921 atacgccgcc actgcggcat ttttttcgcc agccaggctt tcgctttggg taataactga
     7981 caggttgtgc gtggcgctcc ccagcggcta aggctgttgt cgggataagc aaacgcgagt
     8041 atctggtcgt aacgctctcc cttccccgcc tgggccatgg cttgccgcca ggaaagcgtc
     8101 ccttcggtag cgcgactgcc gtgataatga accggatcgc ccgcatagat gagatcctgc
     8161 gtccagacgg tcatcgttcg cgcccccgtg gtagctggcg aagcagaaac acgctgcgtg
     8221 gcgctgctgt cggggatggt caggcaatcg ccttcccgat tggcgttttg ctgtaaaaaa
     8281 gtgcgtatcg ccacagtcat ggctttcgct gcctcaggtg gcgtactttt cgcctcgcga
     8341 tccagaactc tggcaacgta ctcttcacga tcgagatgcg actgcaattt cagtttgccc
     8401 ttttcggtca gtaaggtcgt ctcaccgtgg ctgacaaacg taatgtggtt accattggca
     8461 aacgtcacac gatagcgccc attaagtacg ccgggtttta ctgccgtcgt actcttctcc
     8521 gctgtgattt tcttcagcgg ctagcgcgca aatagctcca cctctacaca ttgcccactg
     8581 gcaacttgcg tcggcactgg caaaacccga ttaagcaccg tcgcataacg ggtcagtacc
     8641 gttttgctgg tgccactgcc agtcacccac agcggtgtac catcggttaa ccagccagca
     8701 aaaccaccct gacggatttc ctgtttatca tcggcaaacc agctccaggt tttcacccgt
     8761 acccgactac cgagcataga tgcgacaccg attttcgcct cgtccagcac cacatccagt
     8821 aagacttcct gagctttgtt ttgcgcaggt aacgtcgcta atgaatcaag cagcgattta
     8881 accgttaccg aggtttctgg cttcagcgta gaaagagacg ctaaccacgc aggtgcctga
     8941 cgctgttgcc agtattgccc ccacacatct gccccaatat gcaaccgctg cggcgcaaaa
     9001 tagagcccac aggaacgtac cagcgcggta tcgcgcgtta tgctctcccc agcctgacaa
     9061 caataaacct cttccgggga gtttccgcga cactggtagg gctgctccgg ttgatgggta
     9121 tcctccagcc aggcgtaaac atacagtttc cataaactcc ctaacggcgt ggtgagcgtg
     9181 tcaggtaatg ctgagacttt ggtcacgcct gatgacgaca gctgatacag ttgatcgtgc
     9241 tgcgcaccgc gcagcgccag ttgtaacggc gtctcctccg caagcgttgg cagagtaacc
     9301 aacgccaata gccagacgat tctgcgccag ttcacttatt tcacctgcat cccggtccat
     9361 tcgctccctg ccgcaacact ttgctgtgca ggtgcatagg aacgcacata acgagcagga
     9421 ggcagaacga attgcccttt ttgcgagaag cgcagcaaat ggcggaaagt gaccgttccc
     9481 gtcagttctt tcaccggcac catataggcc agttcgccca tttcatttcg cgctttttcc
     9541 agcaattgcc cctgctgttt tccagcgttg ggtttattga ctgaaatgcc ccatgttgtg
     9601 cgctcaacgt cggctcccgg cgggagcggt acttccacct gaccatagcg cagaactgca
     9661 tcctgctcgc tggtaagcgt aatttcatcg agatacagcg catcgctgtc aatctcattg
     9721 ctggtcaccg gttgcagagt aaagctcatc tcttcttcac cagggataag ccgatacaat
     9781 tggcgttcaa cggtcaccgg aatgttactt tgttgagccg tttttgccgc ttcacgccag
     9841 cggacctgca cattttgcgg cgataattcg tcaccaaaag agagaatgtc cggcacgccc
     9901 tgaccaaccc aacgccagta ttcgccccct ccagttaact tatgtttggc ccatgcgccc
     9961 gcaggcgcag gcaacacaac cgaaggcatt gtcgccatat atttcgccag ccagttcatg
    10021 gccagcgcgc gctcaatagt agattgctca gcggtcaaac cacttaaaat agcagccgcc
    10081 tgggtagcgt cgcccccgct attgagcagc acaaccgtgt gtgccagcgg ctgattgctg
    10141 tttaccgctt tatcccacgc atattgctga acctgtggca tcaccgtgga cttcaactgc
    10201 gcttttttca gcaacgtcat ggttaagacg tttgccaccg catctgccag tggagattcc
    10261 ggtgtatcga ggatcaggct gtcattgata tcgcgggtgt cttcctcaga gaaatcttca
    10321 gttttagttc cgcgccgggc gatagcttca tccaaccctt tcaacaacgt tttgcacggc
    10381 agattcatct cctgtgccca cgccagcacc agcgcccgat gcaataacgg catgttatct
    10441 gcctgctcgg cataactgtc gagcatatgc tgccagtatt ccggttgttg cgttacgccg
    10501 agcgcctggc tggcctgcca gtcggcgtac catgcccatg ccgtaaggaa ggcgtcacca
    10561 ttgccatctt caccccacca ggtaaagcgc gctccgggcc ccgccagttg catcagccgc
    10621 agacggttat cctgaatcat ctgacgaatg tcgttagcgg cggcactttg atgatcggca
    10681 agcgaacgcc aggcgagact gagcgggatc agacggctac cggtgttgat cacccccccc
    10741 cacggttcat cgactaacgc atcaagattg ttgcggaaaa tctcctgcgg cgtttcacta
    10801 ctttgtagcc ggatattgct cgcctgctcg ggcaacatca gcgcgttatc gccaccgccg
    10861 agcatgacat ttttctgctg ttcaacgggc cagctgttat ccacaaaaga cagttttgtg
    10921 ctaatgctgt cctgcacttg cccattttgt tgcagttctg cgcttaacaa gccagattgc
    10981 tgaatgttct gcgccagcga aatataattc gcccctttgt gcagcgtcag cgtctggcgc
    11041 atctcagcgc ctgcaaattt agtcaccagc gccaccggtt cgttatcctg ctgactgaag
    11101 ataaacagtc ctgccgacgg tttgtcgccc acgcgataca ccgttggcat actccacttc
    11161 atgtagagat ttttttccga acgcagataa gcacgcccct gcccgaccag cccgtcgccg
    11221 ttcatcccac gcgcggtgat acgccagcgg gttaacgaat caggcatcag gaacgtgaaa
    11281 tacgctttgc cttgtttatc ggttgtgagt gacggcatcc atgccgcggt atccacctct
    11341 tcacgccgtg gacgttcaag catttttact cgccgctcgc tgcggttagt tgcgccaggc
    11401 gcaaccggct cgctggagag tgcctggtcg tagctgataa acgacagact ggagctggta
    11461 cgcacattgt tacgccccag cggatagaaa aatttgccga tattcggcgc gatttctggt
    11521 tgcagcgcgt agatcatttc atcgaccacg cctaccgtta gctgcgcaga aacaggttta
    11581 cctttcagcg acgaggttaa ttcgacattg accagttcac caggctggta atgggttttg
    11641 tccgttttca cccggatatc cagctgggga acggcaactt tgatcccggc gttctgaaaa
    11701 ctgtactgac cgttacgggt atacagcacc gaaaaagtga tgttaggcgc aaaggaattg
    11761 ctcactggaa cacgggcttc atattgagta tcgtttaaac gttgtaacgt cagccagttt
    11821 gccggatgcg atagcagcga ctgctgttca acgcgatcgc gttccagcgt caataatgct
    11881 tcatcaattg gctccgggaa ggtaatcagc atcttcgcgg tttcgccagg ctggtacagc
    11941 gttttatctg ccacgatatc taccgtacca gtatgcgaca tactgccctt accgctgacg
    12001 gcatggctta acccggcgag aattaagccg tctttatcgc gtaacgtcag attgtagttg
    12061 ccaggtttat cgaaattgac ggtaaaggat ttgccgcctg acggtagatc tccgctatgg
    12121 ctcgtgcggt cttcgagacg caaccattca tacgtaacag gaacctgttt tgaagattcc
    12181 agcgcggcat aacggaacac aaccgactcg ccgctattac tgtattgtgc ggcggtactt
    12241 aaagagtaat gcgccaggcc gcgttcaatg aggatctctt tggtggtggt gacgcgatac
    12301 gccgcgccgt cactggcgga gacggttaac aaatagcggc tcggtttatc ggcggcgggg
    12361 agatttaacg ccacatgacc gctggcgtcg gacaccgttt cgctgccttc cagcgacacg
    12421 gggaaacgtc cggcataacg caaatcgtta ccgaccattg ataattgctg agcgcgcaaa
    12481 cttaactgca cgcgggcatt ttttaccggc tcgccatccg ggtagagaag ttgcagtttg
    12541 ccgctgaccg cttcgccagt tttgaactct tttttgtcga gagctaaacc aatctcgaaa
    12601 tgtggcttga tgtagtttgc cacgcgaaaa ctgctgctat agacctgatt gcggtaagca
    12661 agacgtaact cataacctcc ggcaacggca ttttctggca ggcggaaact tccctgcccg
    12721 ccattgcgcg catccagcgt gacatcgacg gtttgcaaca gactgccgtt agcgtccagc
    12781 accgaaagct tcgccggggc gctgacgatg ggggatgaat gcaacggatc gtggaactcg
    12841 cggcccatca ctttaacatc gacacgatcg cctgcgcgat atagcgggcg atcggtaaaa
    12901 atatacaagc gggtgttgta gatttcgctt tcgtagaaga agttctcgga gacaaaaacg
    12961 ccgccttcag catccttacc cagaatgtat gaacgttctg gcgatatatg ttgtaactgc
    13021 aaggtaccgc tgtcatcggt cacaccgcgg gtcatcacgc caagaccgtc agtccacaag
    13081 atctctgagc cgggcttcgc ttcaccctgt tttttacccg cggtccacac cagaagctct
    13141 ttgcctgaca ctttgctaag cgccacggta tcggaaacaa acaccaccgt cgtcgcccga
    13201 tacccaccaa ccatcgcctc gacgaggtac agtcccggct cttgttggcc gagaggaata
    13261 taaatgttac ccggctgcgg cgagatgaaa ttgctggatg cgccttccag ttttacccct
    13321 tgctgcggct cgaccggttt agcctgccat agtggataac ggaactgttc caccagggga
    13381 tattttttca gcggggaaaa ctggttgttc tgtacataac gggaaggttt aataatggca
    13441 ttgccgagct gtaattcggg taatgcctga gtcacattct gccgtgactg agaagagaaa
    13501 gtacgctgca tcacgcggcg agatttgccg taccagttat cccacagcca ggtcagcgta
    13561 ttgttcagcc cgtcgcccag atattgcggt tgcaccacaa tgcgatgcag gtttttctgc
    13621 tggcgcaaaa atgccatcgg gtcaggaata cgatacaggc gaacgtccac gccgccgtac
    13681 tcttccatct gatagcgccg ataatcacgc cccggcgctt ccagtcgcac tttcgactct
    13741 tcactgctgc taaaactgct gtcagcaagc aaaaagaatg ttcccccggc gggcggcgca
    13801 tagttactgg aaggaagcga atcatcagca ttagcaagcc ctgttcccac aagagacagg
    13861 gacaagcacg ctgctatcgc gccagaaaat ttaaacgata aatgccaatg aaattgggat
    13921 tggaatcgtt gggtatccat cgggtgtcct tccatgtcat aagttgttgc agactgactg
    13981 cgcgcattcc gttgtcagtt ttcgtggcgc ttccggtgtg gtagatgacg taacgcccca
    14041 tccagaccat taagtgctgg gcatcgccct gatcgaaaaa aatcatatcg ccaggcagcg
    14101 cctggtttat gtcctggcca ataaactggc tgttgtactg aatcaaatta atcgcggtca
    14161 cgtaggggcc ggttttcccg ttcccctgat tccagttttg cgccagttga cgctgttcag
    14221 gtgttagcgt catctctggc ggcaaatact ggctagataa accgttactt tttagccatt
    14281 tactgtcgtg aactttcagc gtctcgttcg ccgcaaatcg caccaggccc gcacaatcct
    14341 gctgatacca gcgtggactt ggcccctggc ggagctgttc ttgtgcaatg cgtacaaacc
    14401 aggcgcgaaa cagcccggat tgttcgacat tcagcatttc gctatgggca acaacacaac
    14461 acagccagca aatcagcgcc agcagcccgt gcctcatagc ggctgccaga ttatgggtag
    14521 ccactgccag gcggcaccgg gttccatctg ggccagcttc atgacataac gcggttgttg
    14581 agataaagcg tccagcttcg gcattaataa agtttgtgcg gcgttataaa aaaccggttc
    14641 gagattcttc ggcagactgg tcaacgtttc gttacgcagc agtttcgcca cgccttgtgg
    14701 attgatatag agcggaacga tgccatcagt gggtattaca tccaccattg ccgggcgggt
    14761 tttattcagt gtttgcaggg cgttattaac cagcgtgtca tcgagggaga aaagcagcgt
    14821 tttgttttgc atcgccagcg acacgcggaa aaaataatcc gacattaatt gatcgggttg
    14881 cgccgcctgc gctttcggat actgaccgta tcgggaactc acttcgcgac gccagatttg
    14941 cgcttcgccc tgctgagtct ggcttaccgg caaaacacct tctggcgctt tgctttcgtg
    15001 cgcaccaata ttttgcgtaa acagtttccc tggcaattgc gcctgttcgg cagtgccatc
    15061 aaactgaccg acaaacagcg gggtttgcaa ttttgagtct tcataccagc acagccccgc
    15121 ggcaccgtcc aacgccccat tcaacttgtc gttttcctgg ctgatgtgcg aaagcatctc
    15181 ttcggcaata ccgtgtgaat acggcaccgc cacacagaag ctggccccgg caggcatact
    15241 gttccagacc ggcgtaaaat cgaaactggc atctacgctg gcggattcat catttaacgc
    15301 cacaaaactg tgccagccgt cgttacccat ttcgaagcgt acgccagcaa aagaaggcat
    15361 taaccgctgg tagccaaaac ccagcaacct ggcgctggcc acgatgcgct ggcgtactgg
    15421 cgttttttcg gcagcacgct cttccaggcc aaagctggct tgccagcgtt ttttgccgct
    15481 caacaaatca cttgcgatcg ccgtggcttc ggtatcctgc tgatcatctt taaacagcat
    15541 atccgtgctg gaaaacacca gcatcttgtc ctgatatgtc gcgaacatca gggcgttatt
    15601 gccgttatag cgcaactgat aaacaggaat agtttcacta tttatcttga tgctactgat
    15661 ttccgttttg cttaactggc tgtcgctggt agcggcaaat aacaatggct ccagcagttt
    15721 gcttaaacca ctgcgttgga tcagcaccat gtaatgtgaa agatggcctt gtttatcgtg
    15781 ccatagcgcc gcctgcgcgg gctgatctaa gagtgacgaa aagagcttat ctttcagcgt
    15841 gagatcgtgt tcatagacaa tgcgacgaat gctgccttca atgcccagac gatcggcatg
    15901 attctgataa tagaaaacga aatcttcact cagaacatcg tggagaaacg gaatggtgag
    15961 gagatctttg ggaagctggc ttagagaatc gctgtcgaga aagaggtccg gctcattgag
    16021 atcaatttgc aaattattgt gcaccaccag cggcgacaac gttttttctg gcccactgcc
    16081 agcatattgc aacgcccaga cgccagcgga aagcagtgct attgcgccaa aacctacaag
    16141 accatagaac cgccagcctt tcgctttttt tcaccactca ttgccacatt ccttgtgtat
    16201 agccagccat tttttacgga cacagccaaa ctttaccgcg ccctaatacg acaaaagccc
    16261 agactttgca gcctggactt ttcaattcaa aaaagggaga tggctccctt ttggcatgaa
    16321 gaagtaaaat tattcttctt ctggctcgtc gtcaacgtcc acttccggag cgatttcatc
    16381 gtccccttcc gcggcactgc cgtcgatggt atccagatct tcctcgtcaa ccggttcagc
    16441 aacacgttgc agacccacta cgttttcatc ttccgcagta cggatgagga tcacgccctg
    16501 ggtgttacgg cccacgatgc tgatttctga aacgcgagta cgtaccagcg taccggcatc
    16561 ggtgatcatc atgatctggt cgcagtcatc tacctgtacc gcgccaacaa ctaaaccgtt
    16621 acgttcggta accttgatgg agataacccc tttcgtcgca cgcgacttag tcgggtattc
    16681 cgccactgcg gtacgtttac cataaccgtt ctgcgttgcg gtgaggattg cgccatcgcc
    16741 acgaggcacg atcagagaga cgactttatc accttcgcct aaacgaatac cgcgaacacc
    16801 ggtggtgttg cagcccatcg cacggacaga agactcttta aagcgcacca ctttaccttc
    16861 ggcggagaac agcattactt cgtcttcgcc gctggtcagg tcaacgccga tcagctcatc
    16921 gccttcaacc agtttgatcg ccactttacc ggcggtacgc agacggttga actcggtgag
    16981 gacggttttc ttcacagtac cgttagcggt cgccatgaag actttcacgc cttcttcaaa
    17041 ctcggtcacc ggcaggatcg cggtgatacg ttcgtcctgc tccagcggca gcaggttgac
    17101 gatcggacga ccgcgcgcgc cacgagtggc ctccggcaac tggtaaactt tcatcgaata
    17161 gacgcgacca cggctggaga agcacagaat atggtcgtgc gtgttcgcca ccagcagtcg
    17221 gtcgataaag tcttcttctt taatacgtgc ggcagattta cctttcccgc cacgacgctg
    17281 cgcttcgtat tcagaaagcg gctgatattt aacgtagccc tggtgagaga gcgtcacgac
    17341 cacatcttcc tgggtgatca gatcttccag gttgatgtct gcgctgttgg cggtgatttc
    17401 agtacgacgt ttgtcaccga actgttcacg aaccagctcc agctcttcac ggatcacttc
    17461 catcagacga tcggcgctac caagaatacg cagcagttcc gcgatctgat ccagcagctc
    17521 tttgtattcg tcgagcagtt tttcgtgctc aaggccggtc agtttctgca aacgcagatc
    17581 cagaattgcc tgagcttgct gttcggtcag gtagtacaga ccatcacgca cgccgaactc
    17641 tggctccagc cattccggac gcgcagcatc gtcgccagca cgttcgagca tcgcggcaac
    17701 gttgcccagc tgccacggat tagccaccag cgcagttttc gcttctgcag gcgtcggcgc
    17761 atgacggatc agttcgatga tcgggtcgat gttcgccagc gccacggcta atgcttcaag
    17821 gatatgagca cgatcgcgag ctttacgcag ttcgaaaata gtacgacggg tcaccacttc
    17881 acggcggtga cgaacaaacg ccgcgatgat gtctttcagg ttcatgatct ttggctgacc
    17941 atggtgcaat gccaccatgt tgataccgaa agaaacctgc aactgggtct gggagtagag
    18001 gttgttgagc acaacttcac cgaccgcatc gcgtttcact tcaatcacga tgcgcatacc
    18061 gtctttgtca gactcgtcac gcagcgcgct gatgccttcc acgcgttttt cttttaccag
    18121 ttccgcaatc ttctcgatca ggcgcgcttt gtttacctga tacggaattt cgtggacgat
    18181 aatggtttca cgtccggttt tggcgtcaac ttccacttca gcacgggcgc ggatatatac
    18241 cttgccgcga ccggtacggt aagcttcttc aataccgcga cgaccgttaa tgattgccgc
    18301 cgtcgggaag tccggccccg ggatgtgttc catcagccct tcaatgctga tgtcttcatc
    18361 atcgatatac gccagacaac cgttgatgac ttccgtcagg ttgtgcggcg ggatgttggt
    18421 tgccatacct acggcgatac cggaagaacc gttcaccagc aggttaggaa ttttggttgg
    18481 catgacgtcg ggaatttttt ccgtgccgtc atagttatca acgaaatcga ccgtctcttt
    18541 ttcgagatcg gccatcagtt catgggcaat tttcgccaga cggatttccg tataacgcat
    18601 tgccgccgca gagtcgccgt cgatggaacc gaagttaccc tgaccgtcta ccagcatgta
    18661 acgcagcgag aatggctgcg ccatacggac gatcgtgtca taaactgccg agtcaccatg
    18721 gggatggtat ttaccgatta cgtcaccaac gacacgggca gattttttat aggctttgtt
    18781 ccagtcattg cctagtacgt tcatggcgta aagtacgcga cggtgtaccg gcttcaggcc
    18841 atctcggaca tctggcagcg cacggccaac aatgaccgac atcgcataat ccagatagga
    18901 gctcttcagc tcttcctcaa tgttgaccgg tgtaatttct ctcgcaaggt cgctcatcta
    18961 accgctatcc ctctactgta tcccggattc aaaggtcgca aattataaca cagccgcgca
    19021 gattgaggta aacctatacg ctttattcac atccaatgcc tgatatactc gtttgtcttg
    19081 ccaattacgg agtagaagtg ccaatgaatg ccgaaaaatc gccggtaaac cataacgtag
    19141 accacgaaga gatcgctaaa tttgaagccg tcgcctcccg ctggtgggat ctggaaggtg
    19201 agttcaaacc gctgcaccgc attaacccgc tgcgtctgag ctatattgcc gagcgtgctg
    19261 gcggcttatt tggcaaaaag gtgctcgatg tcggttgtgg cggcggcatt ctggccgaga
    19321 gtatggcgcg cgaaggcgcg acggtgaccg gtctggatat gggctttgag ccattgcagg
    19381 tggcaaagct gcacgcgctg gaaagcggca ttcaggtgga ttacgtgcag gaaaccgtgg
    19441 aagagcacgc ggcaaaacat gccgggcagt atgatgtggt gacctgcatg gagatgctgg
    19501 agcacgtccc tgatccgcag tcagtggtca gagcctgtgc gcaactggtg aaaccgggcg
    19561 gcgatgtctt tttctcgacg cttaaccgca acggcaagtc atggctgatg gcggtagtcg
    19621 gcgcggaata tattttgcgc atggtgccta aaggtacaca tgatgtgaag aagtttatta
    19681 aaccggcaga actgctgggc tgggtagatc agacaagcct taaagagcga catataaccg
    19741 ggctacatta caacccgatc actaatactt ttaaactcgg ccccggcgtg gatgtgaact
    19801 atatgctgca cacgcagaat aagtgaggtt gttgtttgga ttcatccggc aaccgcgcca
    19861 atgcctgatg cgacgcttgc cgcgtcttat caggcctaca aatgctctcc gtaggcctga
    19921 ttaggcgttt acgccgcatc cagcaatagt gatggtcaga cagcgattac catttcaccg
    19981 tcatcgacaa aaaccctgcc gtctgggcaa aatcatcact ccctttctgc cacgccacgc
    20041 taccacgcag cgaaactcgc tgactgatat tgcccgtgac tcccactttt atttcacccc
    20101 gttgcttcac cgcatcgtca ctgatagtgc tggcatcttc ttcaatttcc gtcgaatggg
    20161 gatcgtgata ataattcaga tctaatgttg gtatgacatg aacagcggta cgccattcgc
    20221 tgtgtaaacc cagccgcgtc tgaatatcat caccctgcga ttgtgacacg cgcgcacggt
    20281 tagcggcggt aaaatcatcc tgctgcacgc cctgataaat cacctgcgcc tgcggttcaa
    20341 tcaccacacc acgccccggt aaccactgat accccgcttc cagcgaggcg ataatccccg
    20401 acgaatggta atgatccacg ccatcttcat gttcagaaac atcattgcta aaccacgcgt
    20461 actgcaacca gttatccagc caggccccct gcttctgctt accgtgctga aaccagcttg
    20521 aggtcagccc aaccgcataa ccgtggttct ggttatcggc gcgagttccg gtcatactcg
    20581 agcggctgtc gccctggtta tcgctgtagc caccaacaat cccaagcatc cactcgccat
    20641 ccgtgcccca gcgcccgcta aacagatcgc cgctaagttg caccgtagaa gtgtcttcat
    20701 gttgagccag ttgccccgct gctgtgtaat gataatctcc gccgataaca cgtaaattca
    20761 gcgtctgacc atcgccacct gcgtgatcgc gtcgctccat cataaacgcc tgatttgccg
    20821 cccgcaggtt attgagataa ccgccaactt tggcattcaa caccggctgg taagcaggcg
    20881 taggttccgg gtcgggtgtt ggatcaggat cctgcgtggg atcaggatcg ggagtcgggt
    20941 ctggatcagg tggcgatggc ggcgttactt cttgcgatcg cagataccag tcgttgttat
    21001 cttccaccag cgtgtagtca tacgctccca tattgacgta gccgctgcct gccagactga
    21061 actgcgcatt gttttgaaac tgcgtgggat cagctgcgaa atcaaccact ttaatgcctg
    21121 tcgatgtcgg ctcaccaatc cctgtaatgg agttaaccac cacagttgtg ttgccagcag
    21181 tattaccgtt catcaccaat tgatcgctta ccgagtcatc gccgtttaat tcgctatcga
    21241 gcagtagcgt accgccgccg gtataatcac cgtttaccgt cagcgtatcg ccagcgacgc
    21301 cattttgcag gctaaccgca ccggacgtat tggtcagatt gccattaacc gtcacgttgg
    21361 aaagataagt caggctgtca gtttcaaggt tggtattaag ctcacctgtc aacgtcacac
    21421 catcggtggc attcaccagc gtcccatcgc agttaaacag tgaggcatta agggcgacgg
    21481 aagtatcctg cccggtcagg cgcaaaaccg taccatcgct gatgtcgata gtgccgctgg
    21541 aagtagcatc aattgactga atatcctgat ccgcgccagt aacgaacgtt gccccgtcac
    21601 cgacccatag cgacgaagca taagggaaga tatccgcaac atcaccttgc agcgtgccct
    21661 cttctactct caccgctgac tgcgttgtac cgctggcggt cagctccagt gtacccgccc
    21721 ccgttttagt caatgtgcta ccctcatcct gatgctgccc gctgctgtca gccatcagtg
    21781 cgccccactg cgtgtctacc cccgcatcaa ctgccacttc accgtcggcg cgcatttcaa
    21841 tatcacgccc atgctcagtc gttgtagagc gagtatcgac attggcagta atctccatca
    21901 cgctttcttg tggcttatcg gtaaagataa cctggcggtt ataatgggta tatccaagct
    21961 gcgagttgtc gctcaccatc agcgtaccgg aagcgatttg cgttgtgccg aggtattgat
    22021 tgtcatttgc caggctgacc tgcccgtcac ccgcatcgat caccacgtag acgccatctt
    22081 tcccgtcgcc gccgatatgc atatcgtttc cgcctgcaag atctaccaca tcctgactgc
    22141 cgataaccgt tccgctgctg ccactgactt caaggccatc tttgtatgat gtaccgctct
    22201 gccaggtgga gaaatcagag agatcgagca taccgccgtt taacacgatc gactgcggat
    22261 cgtcctggag agcggcaaga tctgccgcgt cgccttccag cgaaagcacc gcaccagcat
    22321 ccaccactat atcgccggtt agcgccatcg actgcgcccc cgccagcaca tagctgccgt
    22381 tttgcgcaat ggtgagctga ccagcacctt cgatggtgcc agcaaaactg ccttgattaa
    22441 cagtaacatt gccaccagca tcgatattta aagtgccatt ctgaaagccc gtcaatgagt
    22501 gcgcaaaggt ttgttgggtg gagccaacat ttagctctgc ctgattctgg tacttatcaa
    22561 tactccctat cgtcagaccg tagcagtctt gcggatcgtc ctggcaatgc gtatcgccga
    22621 cattcatcag ggagttgctg cggcccaggg taacttcacc gttttcaatc tgcatctcac
    22681 cagtaaagtc attgttatct gcattaagta ccagatcgcc ggaacctgtt ttggtgatta
    22741 acccggtacc agcaatagag tcaacagctc cgtcattctc tgtattgcca ataaccagcg
    22801 tttttccgtc ggcaatatca aaggtaactt cacttaagcc gagatacata aagccacccg
    22861 ccgcagagga aggaccatct ccatagcctg ctgcgctatt gttctcatcg actaacacgc
    22921 ctccgttctg gctgtagctg tcatcaacag aaatatcaat aagataggga gccgtcgcgc
    22981 tattggtata tatcgccccg ccataacctt cggcagtgtt atttgtaaag gcagtgttat
    23041 ttattatcgt ataacctgaa ggatgcttgc tgtcgctatt attatctgtg acatcgattg
    23101 cgccgccatc gccatcactg taacttgttg atgtatatgc ctggttattg ttaaaaacat
    23161 catcacttaa atagatatca ttattgatgg tataaattgc accacctttg ccgtcattag
    23221 cgatgttatt gcgaaacacg gcgttagtga cacgtaaatc gatggcaccg gtatcgttag
    23281 taccagaaga atagattgcg ccaccatagc cgcctgcgac gttaccagaa aaaataacat
    23341 ccgtaagatt cagcgttgag ttttctttag caaatattgc cccgccgtta ttatattctc
    23401 ctgaaacgat gttattggca aacagagtca ttccagtttc attttctggt gataatgtaa
    23461 attccgctcc ttgttgcaaa aatacggccc caccgctggc attattggtc atatccgaaa
    23521 aaaccagcca ttgcccatcg gcaatactcc agacctgcgt aatgcctgaa aggctttgcc
    23581 tgctggcgtg acaactcgct ttgatattat atccctggca taaatcaatg gccgccgcga
    23641 caccgttagc agggaaaaga gatgcaatca ttgacgggag tagagataaa tactccttgc
    23701 gtagaaagat aacccgc
//
LOCUS       DBFIFI010000059        23215 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.059, whole genome shotgun sequence.
ACCESSION   DBFIFI010000059 DBFIFI010000000
VERSION     DBFIFI010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23215
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.059"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..1079
                     /locus_tag="N7B39_002167"
     CDS             <1..1079
                     /locus_tag="N7B39_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HGU2422148.1"
                     /translation="YLVETIVIIHKPINGSTLIQTKSNDVSIFYIFRQLSFICLTSLA
                     LFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYNADYSLYVVDYCPDNHTA
                     TWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLASLCCNLLLLTLDEYNYT
                     IWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLTNIYNRRYFFNSVESLLS
                     RPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQQSIHPDDILARLEGEVF
                     GLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTISIGTVFSTGDTRNISLV
                     MTEADKALREAKSEGGNKVIIHHI"
     gene            1260..2750
                     /gene="dgcJ"
                     /locus_tag="N7B39_002168"
     CDS             1260..2750
                     /gene="dgcJ"
                     /locus_tag="N7B39_002168"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HGU2422149.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSILQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKLINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            2793..3296
                     /gene="yeaK"
                     /locus_tag="N7B39_002169"
     CDS             2793..3296
                     /gene="yeaK"
                     /locus_tag="N7B39_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HGU2422150.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGADFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(3297..3401)
                     /gene="yoaI"
                     /locus_tag="N7B39_002170"
     CDS             complement(3297..3401)
                     /gene="yoaI"
                     /locus_tag="N7B39_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HGU2422151.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            3571..4017
                     /gene="yeaL"
                     /locus_tag="N7B39_002171"
     CDS             3571..4017
                     /gene="yeaL"
                     /locus_tag="N7B39_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HGU2422152.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIATGLVSLIVGKQ"
     gene            complement(3974..4795)
                     /gene="nimR"
                     /locus_tag="N7B39_002172"
     CDS             complement(3974..4795)
                     /gene="nimR"
                     /locus_tag="N7B39_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HGU2422153.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     YTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RAMVEMMAKEPVEWGALGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPWQ"
     gene            4892..6073
                     /gene="nimT"
                     /locus_tag="N7B39_002173"
     CDS             4892..6073
                     /gene="nimT"
                     /locus_tag="N7B39_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HGU2422154.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            6128..6475
                     /gene="yeaO"
                     /locus_tag="N7B39_002174"
     CDS             6128..6475
                     /gene="yeaO"
                     /locus_tag="N7B39_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HGU2422155.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYSAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(6497..6751)
                     /gene="yoaF"
                     /locus_tag="N7B39_002175"
     CDS             complement(6497..6751)
                     /gene="yoaF"
                     /locus_tag="N7B39_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HGU2422156.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRLDHPAGEK"
     gene            6934..7959
                     /gene="dgcP"
                     /locus_tag="N7B39_002176"
     CDS             6934..7959
                     /gene="dgcP"
                     /locus_tag="N7B39_002176"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HGU2422157.1"
                     /translation="MSDQIIARISQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNQKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHSAPHS"
     gene            7992..8090
                     /gene="yoaK"
                     /locus_tag="N7B39_002177"
     CDS             7992..8090
                     /gene="yoaK"
                     /locus_tag="N7B39_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HGU2422158.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            8093..8167
                     /gene="yoaJ"
                     /locus_tag="N7B39_002178"
     CDS             8093..8167
                     /gene="yoaJ"
                     /locus_tag="N7B39_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HGU2422159.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(8226..8474)
                     /gene="yeaQ"
                     /locus_tag="N7B39_002179"
     CDS             complement(8226..8474)
                     /gene="yeaQ"
                     /locus_tag="N7B39_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HGU2422160.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(8621..8803)
                     /gene="yoaG"
                     /locus_tag="N7B39_002180"
     CDS             complement(8621..8803)
                     /gene="yoaG"
                     /locus_tag="N7B39_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HGU2422161.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(8807..9166)
                     /gene="yeaR"
                     /locus_tag="N7B39_002181"
     CDS             complement(8807..9166)
                     /gene="yeaR"
                     /locus_tag="N7B39_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HGU2422162.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(9339..9977)
                     /gene="leuE"
                     /locus_tag="N7B39_002182"
     CDS             complement(9339..9977)
                     /gene="leuE"
                     /locus_tag="N7B39_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HGU2422163.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGMATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(10104..11027)
                     /gene="dmlR"
                     /locus_tag="N7B39_002183"
     CDS             complement(10104..11027)
                     /gene="dmlR"
                     /locus_tag="N7B39_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HGU2422164.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            11130..12215
                     /gene="dmlA"
                     /locus_tag="N7B39_002184"
     CDS             11130..12215
                     /gene="dmlA"
                     /locus_tag="N7B39_002184"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HGU2422165.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGSATTPQVADAICKIILR"
     gene            12466..14076
                     /gene="yeaV"
                     /locus_tag="N7B39_002185"
     CDS             12466..14076
                     /gene="yeaV"
                     /locus_tag="N7B39_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HGU2422166.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGGAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            14108..15232
                     /gene="yeaW"
                     /locus_tag="N7B39_002186"
     CDS             14108..15232
                     /gene="yeaW"
                     /locus_tag="N7B39_002186"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HGU2422167.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            15288..16253
                     /gene="yeaX"
                     /locus_tag="N7B39_002187"
     CDS             15288..16253
                     /gene="yeaX"
                     /locus_tag="N7B39_002187"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HGU2422168.1"
                     /translation="MSDYQMFEVQVSQVELLTEQVKRFTPVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(16307..17422)
                     /gene="rnd"
                     /locus_tag="N7B39_002188"
     CDS             complement(16307..17422)
                     /gene="rnd"
                     /locus_tag="N7B39_002188"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HGU2422169.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(17504..19189)
                     /gene="fadD"
                     /locus_tag="N7B39_002189"
     CDS             complement(17504..19189)
                     /gene="fadD"
                     /locus_tag="N7B39_002189"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HGU2422170.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTLVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVSPGQPGELCVRGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(19394..19975)
                     /gene="yeaY"
                     /locus_tag="N7B39_002190"
     CDS             complement(19394..19975)
                     /gene="yeaY"
                     /locus_tag="N7B39_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HGU2422171.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQATGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGHGGWGWYNPGPARVQTVVTE"
     gene            complement(20015..20710)
                     /gene="tsaB"
                     /locus_tag="N7B39_002191"
     CDS             complement(20015..20710)
                     /gene="tsaB"
                     /locus_tag="N7B39_002191"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HGU2422172.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(20768..22678)
                     /gene="yoaA"
                     /locus_tag="N7B39_002192"
     CDS             complement(20768..22678)
                     /gene="yoaA"
                     /locus_tag="N7B39_002192"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HGU2422173.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            22810..23154
                     /gene="yoaB"
                     /locus_tag="N7B39_002193"
     CDS             22810..23154
                     /gene="yoaB"
                     /locus_tag="N7B39_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HGU2422174.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         5681 a         5925 c         5655 g         5954 t
ORIGIN      
        1 tctatttagt tgaaaccatt gtaattatcc ataaaccaat taacggcagt acactgatcc
       61 agacaaagtc gaatgatgtt tctattttct atattttccg ccaactcagt tttatttgtt
      121 taacctcgct ggcgctcttt tgttatggaa aagacaacat ccttgacaac aataagaaaa
      181 aaactggcat tctgttgctg gcgctgatcc cttttttagt ttttcccctt ctggcacaca
      241 atctgagcag ttataacgct gactattctt tgtatgtcgt cgattactgt ccggacaatc
      301 atactgcgac ctggggaatc aactatacaa aaatattggt ttgtctgtgg gcatttttac
      361 tgttctttat tatcatgcgt acacgattag ccagcgaact atggccgtta atagcattat
      421 tatgtctggc atcgctatgc tgcaacttac ttctactgac tctggatgag tataattata
      481 ccatctggta tatcagtcgc gggattgaag tctccagtaa actgtttgtt gtgtcttttc
      541 tgatttataa catttttcag gagctgcaac tctccagcaa actggcagtt catgatgtgc
      601 tgaccaatat ttataatcgg cgctactttt tcaacagcgt agagtcatta ttgtcgcgac
      661 ctgttgttaa ggacttctgt gtcatgctgg ttgatattaa tcagttcaaa cgcatcaatg
      721 cccaatgggg acatcgtgtg ggtgataaag tgctggtttc aattgtcgat attatccagc
      781 aaagcatcca ccccgatgat attttagcgc gactggaggg tgaggtgttt ggcttgctat
      841 ttaccgaact caatagtgcc caggcaaaaa tcattgcgga acgtatgcgt aaaaatgtcg
      901 aactcctgac cggctttagt aacagatatg atgttcctga acaaatgacc atcagtattg
      961 gcacagtttt ttcaacgggt gacacgcgta atatctcgct tgtcatgacg gaagcagata
     1021 aagccttacg cgaagcgaaa agcgaggggg gcaacaaagt gattattcat catatttaag
     1081 tgcaaaaata ttcagagcca tgctttttgc gtggcttttg catacaattt attactatta
     1141 cccctaaatt tcccctcccc agcggcgcag agatgagtat aattagcgcc cctgtgccag
     1201 gccgcaatcg aactttatct ggttttctcg tttcactaac cgaaggagtg ccatttatca
     1261 tgaaattgca ccatagaatg ctccggcatt ttatcgccgc aagtgtcatt gtgctgacat
     1321 cttccttcct tatttttgaa cttgtcgcca gcgacagagc aatgagtgcc tatctgcgct
     1381 atatcgtgca gaaagcagac tcctcctttc tttatgataa gtatcagaat cagagtattg
     1441 ccgcgcatgt gatgcgcgct ctcgctgctg agcagtcgga agtgtcgcca gaacagcggc
     1501 gcgccatctg cgaggctttt gagtctgcca ataataccca tggcttaaac ctgactgccc
     1561 ataaataccc gggcttacgc ggcacattac aaaccgcatc cactgactgc gacacaattg
     1621 tggaagctgc agcactatta cccgcttttg atcaggcagt ggaaggcaac cgccaccagg
     1681 atgattacgg ttcaggtctt gggatggccg aagagaaatt tcactattat ctcgatctca
     1741 atgaccgcta tgtctatttt tatgagccgg ttaatgttga atactttgcg atgaataact
     1801 ggtccatcct gcagtcagga agtattggca tcgatcgcaa agatattgaa aaggtattta
     1861 ccgggcgtac cgtattgtcg agcatttacc aggatcagcg tactaaacag aacgtgatga
     1921 gtttgctgac gccggtgtat gttgcagggc agctaaaagg gattgtgctg ctggatatta
     1981 acaaaaacaa tctgcggaat atcttttaca ctcatgaccg ccctctcctc tggcgttttc
     2041 tcaatgtcac gctaaccgat acagattcgg ggcgcgacat tatcatcaac cagagcgaag
     2101 ataatctgtt ccagtatgtc agttacgtcc atgacttacc gggcggcatt cgtgtctcgt
     2161 tatccattga tattctttac tttatcacgt cttcgtggaa aagcgttctg ttctggattt
     2221 tgacggcgtt aattttgctg aatatggtgc ggatgcactt ccgcttatac caaaatgtgt
     2281 cgcgagaaaa tattagtgat gcgatgactg gactgtataa tcgcaaaatt ttaacccctg
     2341 aactggagca gcggttgcag aaactggtgc aatccggttc ttcggtgatg tttattgcaa
     2401 ttgacatgga caagttaaag ctaataaatg acaccctcgg tcatcaggaa ggggatttag
     2461 cgattacgtt gttagctcag gcgattaaac aatcgattcg taaaagtgat tatgccatcc
     2521 gactcggtgg cgatgaattc tgcatcattc ttgtcgattc gacgccgcaa attgcagcac
     2581 aactgcctga acgtatcgaa aaacgtctgc aacatatcgc gccgcagaaa gagatcggct
     2641 tctcttccgg tatttacgcg atgaaagaaa acgatacgtt acatgatgcg tataaagctt
     2701 ccgatgagcg tttatatgtc aataagcaga acaaaaacag ccgttcatga taaccttctg
     2761 tggttgtttg cttgtaatct caggagcgtg aaatgactga aatggctaaa ggaagcgtga
     2821 cgcatcagcg gttaatcgct ttattatcac aagaaggtgc tgatttccgc gtcgttaccc
     2881 atgaagcggt aggtaaatgt gaagcggtgt cagaaattcg tggcaccgct ctaggccagg
     2941 gtgctaaggc actggtatgt aaagtcaaag gcaatggcgt aaatcaacat gttctggcga
     3001 tcctcgcagc cgatcaacag gccgatctga gccaactcgc cagccatata ggcggattac
     3061 gcgcttcgtt ggccagcccg gcggaagtcg atgaactcac aggctgtgtc ttcggcgcga
     3121 tccccccttt cagcttccat ccaaaactca aactggttgc cgaccctcta ctcttcgaac
     3181 gatttgatga aatcgccttc aatgcaggga tgctggataa atccgttatt ttgaaaaccg
     3241 cggattattt gcgcattgcg caaccagaac tggtcaattt ccgccgcact gcgtaactag
     3301 cctgtccgtt caataagcag aacggacaaa accagcacaa cagcaatggc aaaaaacgat
     3361 gacgtgataa tcagtgtctc gacgaacatt tgatcgttca tagcataccc ccgggagata
     3421 gctgttttca cgttattgtt agcgtgcaca aatggcagtt tgatgacagt tcgcctgatt
     3481 ttttatgcaa aaaacgtaaa gattttctac ttccttcctg cagcaagcgt aaagtaagca
     3541 ggcttattat tttttggcaa ggaaaccacg atgtttgatg tcactttgct gatcctgctc
     3601 gggttagctg cgctgggctt tatcagtcat aacaccactg tcgccgtttc aattctggtg
     3661 ttgatcattg tccgcgtcac accgttaagc acctttttcc cgtggattga aaaacagggg
     3721 ctaagtatcg ggataatcat tctgacgatt ggcgttatgg cccccatcgc cagcgggacg
     3781 ctaccgccct caaccttgat tcactcgttt ctgaactgga aatcgctggt ggcaattgca
     3841 gtaggcgtta ttgtctcctg gctgggtggg cgcggcgtga cgttaatggg cagccagccg
     3901 caactggttg ctgggctgct ggtgggcact gttttaggcg tagcgctgtt tcgcggcgta
     3961 ccggttggcc cgcttattgc cacgggtctg gtttcgctga ttgtggggaa acagtagtta
     4021 atcccgcaat atatcggcct ggcgtttgcc ccaggccttt cttaaacata gtaataaagg
     4081 ccgttgttga atcatacccc agcgtatgag caactttctg tacagtatcg cctttcacta
     4141 atccctgaag cgccataatc agctgtaact gctgtcgcca ctggcgaaaa ctcaaccccg
     4201 tctcttttac tatcagtcgg gcaagattgc gttcactcat cgcaaaaaag ccagcccatt
     4261 gccccaatgc cccccactcg acgggctctt tcgccatcat ctccaccatc gcgcggattt
     4321 taggatgaga agaaacgggt aggtgcagct gttgttgtgg ctgctgaggg agttcatcaa
     4381 acaggacctg aattaatctg cgggtcatcg gctcagcacg ctgagtcgtc gtcctgtttg
     4441 ccagagttaa aattaattcc cggcataagg gcgatatttt caatgtgtaa catgttgtcg
     4501 gcattgtcac tgcgctgggt tcgataaaca gaaaacagag ttcagcgtta gctgtcactt
     4561 gattgctatg ttccaccccg cccggtatcc aaaccgcata ttgtggcggc accatccaca
     4621 aagcattttc caccgtacag gtaattgcac catgtagcgc cagtatcagc tgtcctttgc
     4681 ggtgctgatg tacaggactg gttaactcat ccgttccggc gtgaatacaa aatgcgaccg
     4741 cggcttcatg gtgtcggtcg ggttcatagc cattgagatt caacctgtgc atcattttgt
     4801 ccgaatttag cgataatttg tcattttagc ttgattcaac ataacaataa aaacggtaag
     4861 gtacagcctc gtttgtaaca atgagaagca tatgacctgt tcaacttcat taagcggcaa
     4921 aaacaggatt gtccttatcg ctggcattct gatgattgcc actacattac gcgtcacctt
     4981 caccggcgca gcaccgttac tcgatacgat tcgttccgct tactcgctga cgactgcgca
     5041 aaccggctta ttgaccaccc tgccattatt ggcctttgcg ctaatctcac ctctggctgc
     5101 cccggtggct cgacgttttg gtatggaacg tagcctgttt gccgcgttac ttttgatctg
     5161 tgcaggtatc gcaattcgct ctctcccttc gccttactta ttatttggcg gtacagcggt
     5221 cattggcggt gggattgcat taggtaatgt cttactgcca ggattaatta aacgcgattt
     5281 ccctcattcc gtcgccagac ttaccggcgc atattccctg acaatgggag ctgcggcggc
     5341 tctgggatcg gctatggtag tgccgctggc tttgaacggt tttggctggc aaggcgcgtt
     5401 gctcatgctg atgtgttttc ctctgctggc tcttttttta tggctgccac agtggcgaag
     5461 ccaacaacat gcaaatttga gtacttcacg cgccttacat actcggggta tctggcgttc
     5521 gccgcttgcc tggcaggtaa cattgtttct tgggatcaac tcactggtct attacgtgat
     5581 tattggctgg cttccggcga tcctcatcag tcacggctat agcgaagcac aggcgggttc
     5641 actgcatggt ttgctgcaac tagccacagc agctcccggt ttgctgatcc cacttttctt
     5701 acatcatgtg aaagaccagc gtggtattgc agcgttcgtt gccttgatgt gcgcagtggg
     5761 cgcggttggg ctctgcttca tgccagcgca cgcgatcacc tggactctgc ttttcggttt
     5821 tggttccggc gcaacaatga tactggggtt gacgttcatt ggtctgcggg ctagttctgc
     5881 gcatcaggcg gcggcactct cggggatggc acaatccgtc gggtatttgt tggcagcctg
     5941 tgggccgccg ctgatgggta aaatacacga tgctaacggt gactggtccg taccactaat
     6001 gggtgttgca atactttcac tactgatggc gattttcgga ctttgtgccg ggagagacaa
     6061 agaaattcgc taatatccgg tactatagtg acgtgacaaa tcatgcgtga aagggagaac
     6121 aaacacgatg aatattcagt gcaaacgcgt ttatgatccg gcagaacaga gcgatggtta
     6181 tcgcgtactg gtcgaccgcc tctggccacg cggtatcaaa aaaaccgatt tagcccttga
     6241 tgagtgggat aaagaaatca cgccgtcaac ggaactgcgc aaagcctttc acggcgaagt
     6301 cgtcgatttt gcaacctttc gcgagcaata tcttgcagaa ctggcacaac acgagcaaga
     6361 aggaaagcgg ctggcggaca tcgccaaaaa acagccgctg accctgctct actcagcaaa
     6421 aaacaccacg cagaaccatg cgctggtgct ggccgactgg ctacgtagct tgtgatttta
     6481 gtacagcatc cggcggttat ttttcaccag ccggatggtc cagtcgccac aatgcccatt
     6541 catcaatcgt ttcaccgccc ggtaatttgc aattgttgct gaccccttgc gctgtctgca
     6601 ctggaatgag cgtcccgccc ttctgctggc aatagaccga cgccggattt gccataccaa
     6661 tctgcggcgg tttaggtgct tctggctgag aaggggttga acaaccagcc aggaccagca
     6721 agcaaggcag aacaaaactg ataattttca tttattgatc tcacatatgt atccaagctt
     6781 agagtatcgc ggtatcgttt tgttttgcag cactattttt attacattca ctcaaaacat
     6841 attacgtctt gtttcatctt tgttgatgat gttttatcat gcctgcaaag attaaataat
     6901 cagcatttac ccgccgtatc ctggagttgt ttcgtgtcag atcagattat cgcccgcatc
     6961 tcgcaatccc ttgccaaaga acagtcactg gaaagtctgg tccgacagct tctggagatg
     7021 ctggaaatgg tcactgatat ggaatcaacc tacctgacca aagtggatgt cgaagcgcgc
     7081 ctgcagcata ttatgtttgc ccgtaacagc cagaaaatgc acatcccgga gaattttacc
     7141 gtctcgtggg attactcgtt atgcaaacgc gccattgatg aaaactgctt tttcagcgat
     7201 gaagtccctg accgttgggg cgactgtatt gcggcacgca atcttggcat caccacattt
     7261 ctgagcacgc caattcactt accggatgga tcattctatg gcacgctttg cgccgccagc
     7321 agtgagaagc gccagtggag tgaacgcgcg gaacaggttt tacagttatt cgccggactg
     7381 attgcacaat atattcaaaa agaggcactg gttgaacagc tgcgcgaagc caatgctgcg
     7441 ctgattgcgc aatcgtatac cgactcgtta accgggctac cgaatcggcg ggcgattttt
     7501 gaaaatctga cgacgctgtt ttccctcgcc cggcatctta accaaaagat aatgatcgcg
     7561 tttatcgatc tggataactt caaattaatc aatgatcgtt ttggtcataa tagtggcgat
     7621 ctgtttctca ttcaggttgg cgagcgcctt aatacgctcc agcaaaatgg cgaagttatt
     7681 ggtcgtctcg gcggtgatga gtttttggtt gtttcactaa acaacgagaa tgcggatatt
     7741 tcgtcgctgc gagaacgcat tcagcagcaa atacgcggag aatatcactt aggtgatgtt
     7801 gatttgtatt atcctggtgc cagtcttggc atagtagaag tcgatcctga aacgaccgat
     7861 gcagacagtg ccctacatgc tgccgatatc gcgatgtatc aggagaaaaa acacaaacag
     7921 aaaacacctt ttgtcgcgca ttcagcgcca cattcctgag gcgtattcac atccttttga
     7981 ttggtgataa catgcgaatc ggtattattt ttccggttgt aatcttcatt acagcggtcg
     8041 tatttttagc atggtttttt attggcggct atgctgcccc gggagcataa agatgaaaaa
     8101 aacaacgatc attatgatgg gtgtggcgat tattgtcgta ctcggcactg agctgggatg
     8161 gtggtaacgt cacatctaaa aaatagcaaa ggctgcctgt gtgcagcctt tgtgcaattt
     8221 acgcgttaac ttttaatctt cctgtagata aatagcacga caatcgcacc aataacggca
     8281 accacgaagc tgccaaaatt gaagccatcg actttaccaa agccaaacag cgtgctgatc
     8341 caaccgccga ctacggcacc gactatcccc agcaggatag tcataaagaa tccaccacca
     8401 tctttacctg gcatgatcca cttcgccaga ataccggcaa taagcccaaa aataatccat
     8461 gacagaatgc ccattgtttc ctcacttatc tgttttgcat tagcgggtta gtcgctgata
     8521 aaaagcatag cacaacatcg ggagggcaag atttgtgacg agcatcacgg aggttttttt
     8581 gcgatggcgc agaaattgcg ccatcaacga tcagtgataa ttaccaacca caaacatcat
     8641 gttcgttttc cgtgtcataa gaacgcacgg tattcaccag atcttttatc acttcagccg
     8701 ccacttctgg caccagcaaa gtcatcggcg tctctgtttc ataatcgaca gaaacgccat
     8761 tgctgttatt ggtgacggtc acggtatacg ttgctttgcc catgattcat ttcccgttat
     8821 gaatgacttt ccgttgttgc gcaccttcca tcaggacttc aggagccacg aagaagtcaa
     8881 tgttgaaata agtatcgtca gtcatggctt caatgttgtg ccacttttct ggagggaaca
     8941 ccgcaaactg ccccgcttcg ataaggatca cctgatcagg ctctgcactg tgttcatcag
     9001 cgtagccgag atatttgacc gccccatgca taacggaaag gcgtgggtaa acccccgggc
     9061 gcgttccttt atcaagatga cgttcgaata ttccggcagg cgcagtttgt ttattccaga
     9121 aaggcgttga gcgcgtatga atataattct gtgggatttg cagcatcctt ttccctcctt
     9181 cggtgaatgc gctgaaaacg gtttattcca gccgtttcag ggtacgcctg ataatttgct
     9241 ttttaaatac catttattgg ttacttttta gcaccatatc agagaaaaat cagggaggat
     9301 tatagatggg aagcccgcga caacgcgggc tgaaagcatc aggattgcag cgtcgccagt
     9361 cgggcagcga aacccacgaa catcaaacca atcagtgagt tgcccacttt agccagtttc
     9421 tttttggtac gtatgtactg cgtgacaaaa gcaccagata taatcaggaa gctcaaatag
     9481 cagaaactca ccagttccag cgtcgtcgcc agaataaaga atgaaattcc cgtatgtggg
     9541 gcattaacat cgataaactg tacgaaaaac gacacataga acaaaatggc tttcggatta
     9601 gtcaggctca aaattaacgc gcgtttaaaa atagcaccgt attggggctc atcggatttg
     9661 gcctcgttat ttttaccctt cagggtcgcg taaagaattt tactccccag atagagcaaa
     9721 taaaacgcac caagataacg tacaatgttg aataatatcg gggtggtctt aattaatgtc
     9781 gccattccag cccatgccag aaacatcaat accgcatcgc caataaatac accgcaggcc
     9841 gcaagataac cgcctttcat accgctactg acgctatttt tgagtacaaa cagggtattt
     9901 ggccctggca ccaacacaat aaaaatggcc ccaaccagat aggtccagta attcagaacc
     9961 ccgtattcag cgaacacatt aacctcttta attatctttc gatcatgcgc gattaaaggt
    10021 gaatatgcta accaatctgt agcggcttag aaaggagaaa atcaggtttt aacctgatat
    10081 caacccgata attgaatcat taatcaggca tgctccagtg aaaaattcgg gtagtgctcg
    10141 gcaaaatact ggcgtaaaaa ttctaccgtt atccgcactt tcgctgacgt cgccagcctt
    10201 gaaacataaa cggcccagac gttcgctggc tggtaatatt ccggtagcac ttgcactaaa
    10261 tgaccactgg caatgttttc gctaacatcc caccaggaac gcagagcaat cccctgccca
    10321 tcgagacacc actgatggac aatttctcca tgattagacg acagtgggcc ggtgacttta
    10381 atcgcatggt ggccttcttt attgcgtaat tgccagacac caaaaggatg atcgcgctct
    10441 ttaatgacca ggcacggcaa tgcagaaaga tcggttaaat gtttcggcgc gccatgttgc
    10501 gcaataaatt ccggcgacgc gcagagaatg cggtaattgg tcgccagttt gcgggcgatt
    10561 aaattagggg cgatgtcatc gccaatgcga atatcgagat cgacaccttc attgaccaaa
    10621 tcgaccagcc ggtcttccac atcaaaacgt aattccagtt gcggatacgc cttcgccagc
    10681 gctgataacg ccggagccac cacctgtcgt ccaaaaccaa agctgctgat aatacgcagc
    10741 atcccctgtg gcacctgacg cacgtcagaa agttcgtcca tcatctgacc gacatcctgc
    10801 aaaatccgct gcgcccattc ataaatccgc tctccttctt cggtaatggt gacgcggcgg
    10861 gtggtgcggt gtaacaacac cacgtttagc gtttgctcca gcaaggcgat gcgcttgctg
    10921 acgaacgccg gtgaaacgcc cagttcttcc gccacggcgg caaaaccagc ccggcgagcc
    10981 accagcataa agacgcgcaa atcattcagc agcggtaaat tattcatgat tcgtgtttta
    11041 tgtttcacca gttacgggga ttaattcctt ttcagtcaat tataggatgg tgatgttgtc
    11101 aattttgatg gtcaggaagt gagaacccaa tgatgaaaac gatgcgtatt gctgcgatcc
    11161 cgggagacgg gattggcaaa gaagtccttc ctgaagggat tcgcgtgtta caggctgccg
    11221 ctgagcgctg gggcttcgcc ttgagttttg agcaaatgga gtgggcgagc tgcgagtatt
    11281 acagccatca cggtaaaatg atgccggacg actggcatga gcaacttagc cgtttcgacg
    11341 ccatctattt tggtgccgtc ggctggccgg acaccgttcc ggaccatatt tcgttgtggg
    11401 gttcgctgct gaaatttcgt cgtgaattcg accagtacgt caacctgcgc ccggttcgtc
    11461 tctttcctgg cgttccctgc ccgctggcgg gaaaacagcc tggcgacatc gatttttacg
    11521 tggtcaggga aaacaccgaa ggcgaatatt cctcgctcgg cggtagagtg aatgaaggta
    11581 cagagcatga agtcgtcatt caggaatcgg tatttacgcg tcgtggtgtc gatcgcattt
    11641 tgcgttatgc cttcgaactt gcgcaaagcc gcccacgtaa gacgctaact tctgccacta
    11701 aatcaaacgg tttagccatc agcatgccgt actgggatga gcgagtggaa gcaatggccg
    11761 agaattaccc ggagatccgc tgggacaagc agcatattga tattctctgc gcgcgttttg
    11821 tgatgcagcc ggaacgcttc gatgtggtgg tggcgtccaa tttgtttggc gatatccttt
    11881 ccgatcttgg cccggcctgc accggcacca ttggcattgc cccatccgcc aacctgaatc
    11941 cggaacgcac tttcccctcg ctcttcgagc ctgtccacgg ttccgcgccg gatatctacg
    12001 ggaaaaatat tgctaaccct atcgccacaa tttgggccgg ggcaatgatg ctcgattttc
    12061 tcggcaatgg cgatgagcgt ttccagcaag cgcataacgg tattctggca gcgattgaag
    12121 aagtgattgc tcacgggccg aaaacaccgg atatgaaagg cagtgccacc acgccacagg
    12181 ttgccgacgc gatttgcaaa attattttgc gttaaggtca aaccagttta tttgaaccgc
    12241 gtcactgacg cggttttgtt attcgttctt tgcagttaat aacctgcgtc atttcacctt
    12301 ttattgtttc cgtttcgtgt tttatggctt tccgtattct taattgttta atttatgtaa
    12361 catgcaaata atcaattcgt taaacacgac atacaactca ttaccctgca aataattaca
    12421 actggcaaaa gccagagacg ggcttttatg cccaggagat gactcatgat gagcaatgtt
    12481 aagaaaaaag atgtgccgct gataagcatc agcctggtgg ccattctttt catcgcagct
    12541 gcattaagcc ttttcccaca acaatcggcc gacgcggcca acgcaatata cacttttgtt
    12601 acgcgtacgt taggttccgc agtacaggta ttggttttgc tggcaatggg actagtgatt
    12661 tatttagcca ccagtaaata cggcaatatt cgtcttggcg aaggaaaacc ggaatacagc
    12721 acgctctcct ggctgtttat gtttatttgt gccggtttag gttcttctac gctttattgg
    12781 gggggtgctg aatgggccta ttattatcaa acgcctggat taaatatcgc accgcgttca
    12841 caacaggcac tcgaatttag cgtcccttac tccttcttcc actggggcat cagcgcctgg
    12901 gcaacttata cgctggcctc attaatcatg gcttatcact ttcatgtgcg gaaaaacaaa
    12961 ggtctgagtc tttccggcat tattgccgcg attaccggcg ttcgcccgca aggcccatgg
    13021 ggaaaactgg ttgatttgat gttcctgatc gccactgtcg gcgcactgac tatttccctt
    13081 gttgtcaccg cagcaacctt tactcgtgga ctttccgcac tgaccggttt acccgataac
    13141 ttcaccgtgc aggcatttgt gatcctgctt tccggcggca ttttttgcct aagctcatgg
    13201 attggtatca acaacggttt gcaacgtctg agcaaaatgg ttggctgggg cgcgttcctg
    13261 ctgccattac tggtgctgat tgtcggtcca accgaattta ttaccaacag catcatcaat
    13321 gccatcggcc tgaccacgca aaacttcctg caaatgagct tattcaccga tccgcttggc
    13381 gatggttcat ttacccgcaa ctggaccgtt ttctactggc tgtggtggat ctcatacacc
    13441 cctggcgtag caatgtttgt cacccgcgtt tcccgcggtc gtaagattaa agaagttatc
    13501 tggggactga tcctcggcag caccgtcggt tgctggttct tcttcggcgt aatggaaagc
    13561 tatgccattc atcagtttat caatggcgta atcaacgtcc cacaggtgct ggaaactctg
    13621 ggcggcgaaa cagctgtaca gcaagttctg atgtcgttgc cagccggtaa attgttcctc
    13681 gccgcatacc tgggcgtgat gattattttc cttgcctcgc atatggatgc agtggcctac
    13741 accatggcgg cgaccagtac gcgtaatctc caggaaggtg acgatcctga ccgtgggctg
    13801 cgtcttttct ggtgcgtggt gatcactctg atcccgcttt ccatcttgtt taccggtgct
    13861 tcgctggaaa cgatgaaaac caccgtcgtg ctcacagccc ttcccttcct cgtcatttta
    13921 ctggtgaaag tcggcggatt tattcgctgg ctgaaacagg attacgccga cattccggct
    13981 catcaagttg aacattatct cccgcagaca ccggttgaag ccctggaaaa aacaccagtg
    14041 ctccctgcgg gaaccgtatt caaaggcgac aactgagcgc catcatccta acgataaagg
    14101 tatccctatg agcaatctga gccctgactt tgtactaccc gaaaattttt gcgctaaccc
    14161 gcaagaggcg tggaccattc ctgcccgttt ttataccgat cagaacgcct ttgaacacga
    14221 aaaagagaac gtcttcgcca aaagctggat ttgcgtcgct cacagcagcg aactggcgaa
    14281 tgccaatgat tatgtgacgc gtgagatcat tggcgaaagc atcgtgctgg tacgcggtcg
    14341 tgataaggtt ttgcgcgcgt tctataacgt gtgtccgcac cgtggtcatc agttgttgag
    14401 cggtgaagga aaagcaaaaa atgtgattac ctgcccatat cacgcatggg cattcaaact
    14461 cgatggcaat ctggcccatg cacgtaactg cgaaaacgtc gccaatttcg atagcgacaa
    14521 agcgcaactg gttccggtgc gtctggaaga atatgccgga ttcgtcttca tcaacatgga
    14581 ccccaacgcc accagcgtag aagatcagtt acccggtctg ggcgcgaaag tgctggaagc
    14641 ctgcccggaa gtccacgatc tgaaactggc ggcccgcttt accacccgca cgcctgccaa
    14701 ctggaagaac attgtcgata actatctcga gtgctatcac tgtggtccgg cgcatccagg
    14761 tttctccgac tccgtacagg ttgatcgtta ctggcacacc atgcacggta actggacgct
    14821 gcaatacggt ttcgccaaac cgtccgaaca gtcgtttaaa tttgaagagg gtacggatgc
    14881 ggcattccac ggtttctggc tgtggccgtg cacgatgctg aatgtcaccc cgatcaaagg
    14941 gatgatgacg gtcatttatg aattcccggt ggattctgaa actaccctgc aaaactacga
    15001 tatttacttc accaatgaag agttaaccga cgagcaaaaa tcgctgattg agtggtatcg
    15061 cgatgtgttc cgtccggaag atttacgtct ggttgaaagc gtacagaaag ggctgaaatc
    15121 gcgtggctat cgtggtcagg ggcgcatcat ggccgacagt agcggtagcg gcatttccga
    15181 acatggtatc gcccatttcc ataatctgct ggcgcaggta tttaaggact aatgacatcg
    15241 gcggcggtat tttccgccgc cgggctgatt tttgatggag tacagcaatg tcagactatc
    15301 aaatgtttga agtacaggtg agtcaggtgg aactgcttac cgaacaggtg aaacgcttca
    15361 cgccggtggc aaccgatggc aaaccgctgc ctgcgttcac cggaggaagt cacatcatag
    15421 tgcagatgag cgatggtgat aaccagtaca gtaatgcgta ttcactactg agttcaccgc
    15481 ataacacctc tagttatcag attgccgttc gactggaaga aaactcgcgc ggcggttccc
    15541 gctttttgca tcagcaggta aaagtgggcg atcggttaac gatctcaacg cctaataacc
    15601 tgtttgcgct aattccctca gccagaaagc atctgtttat cgcgggcggt attggtatca
    15661 cccctttcct gtcgcacatg gcagagttgc aacacagcga cgtcgactgg cagctacatt
    15721 actgctcgcg aaatccagaa agttgcgcat ttcgtgatga gctagtccag catccgcagg
    15781 ctgagaaagt ccatttgcat cattcatcaa ccggaacacg actggaatta gcgcgattat
    15841 tggcggatat cgaacctggc acacacgttt atacctgtgg ccccgaggcg ctaattgaag
    15901 cggtaagaag tgaagctgcg cgtctggaca tcgctgccga tacgctgcac tttgagcaat
    15961 ttgctatcga agacaaaacc ggcgatgcat ttaccctggt gcttgcccgt tccggaaaag
    16021 agtttgtggt gccggaagag atgactattt tgcaggttat tgaaaataat aaagccgcga
    16081 aagtggaatg tttatgtcgt gaaggggtat gcggaacctg cgaaacggca atactggaag
    16141 gtgaagctga ccatcgggat cagtatttta gcgatgaaga gcgtgccagc cagcaaagta
    16201 tgttgatctg ttgttcgcgt gcgaagggta aacgcctggt gttggatttg tagtttgccg
    16261 aagccggatg tggcgctgag cgcgcccagt ccggcttcgg aaaattttac tgcggatatt
    16321 cctgcaataa attgtgtaat gcttccgcca tcagctcacc acgccagccg gaaatcagct
    16381 ccggcaaatt gttctgcggt ttcagtttcc agtgccagtt cagcagttgg ttgatttgcc
    16441 gacgcgatgc cagcaattcg gcgctgatct tgtgcgtttc gctcacgtca gtaatcagcg
    16501 acttaatcgc tttaaacgct ttacgataac ccggcatgtc catcaggtta agcatcggct
    16561 gcggtaaggc ctcttccggc aatgtctgcg ctttttccac cagcgccagc agcgttttac
    16621 cgtgaaagcg gatttcgcta ccggataaac ccaggctgtc cagttcgcct aaacttcccg
    16681 gcatataacg cgctaccgac cacaaatgct cttcacgcac gacaaagttc accgccagat
    16741 cgcgctctcg cgccttgcgc agtcgccagt cggctaacag ttgcagacag gccagttggc
    16801 gtgtgcgtaa ttgccaggca ttggtgatat cacgccaggc atcttccggc gcaacgactt
    16861 cctgacgacg catttgcatc aggcgacatt catccagcgc cgctggtagc cagccggagg
    16921 cctccgtttc taccataagc ttggcggtga tcggtaacag ataccagaca tccgccgctg
    16981 catattcaca ctgacgttcg gtcagcggtc tggccagcca gtcggtgcgc gattcactct
    17041 tgtccagcgt aacgccggaa tactcttcca ccatggaagc gaaaccccat gacatcgggc
    17101 gtccgcagaa ggcagcaagg atttgcgtgt caatcagggg ttgtggtaat tcgccaaaga
    17161 cattgaggaa cacttccaga tcttcactgc ctgcatggag aaattttgtg atggacggat
    17221 cgcgcaggat cgctttcagc ggtgaccagt cggtgatccc gagaggatca attagcgcca
    17281 gatgctcgcc atcgaaaagt tgaatcaacc ccagctgcgg gtaataagtg cgcgtacgaa
    17341 caaattcagt atccagggct atcgccggaa aggcacggac ggcttcacac aaagaagcca
    17401 gcgcatcgtc cgtggtaatc atttggtaat tcaaattgtt ttctctttag tgggcgtcaa
    17461 aaaaaacgcc ggattaaccg gcgtctgacg actgacttaa cgctcaggct ttattgtcca
    17521 ctttgccgcg cgcttcgtca cgtaattctc gtcgcaaaat ttttccgacg ttagatttcg
    17581 gtaactcatc acgaaactcc accagcttcg gtactttgta gcccgtgagc tgacggcggc
    17641 aaaaggtcac cagtgactct tcggtaagcg atggatcttt tttcactacg aagattttca
    17701 ccgcttcacc actggagccg gaaggtacac caacagccgc gacttcctgt acgccaggat
    17761 gctgcatgac gacatcttca atctcgttgg gatagacgtt aaaaccggaa accaaaatca
    17821 tgtctttttt acgatcgaca atgcgcagga atccttcttc atccattacc gcgatgtcgc
    17881 cagtgtgtaa ccagccgttt ttgatgattt catcggtagc atccggacgc tgccaatagc
    17941 ccagcatcac ctgtggacct ctgacgcaaa gttcacctgg ctgacctggc gatacttcat
    18001 tatcgtcgtc atccaccagt ttggcttccg tcgacggcac cggcaaacca atgctaccgc
    18061 tatgataatc aatatcatag gggttcacgc tgaccagcgg cgcacattcg gtcaggccat
    18121 aaccttccag cagatactgt ccggtcagtt tcacccagcg ttctgccacc acttgctgaa
    18181 caggcatccc accgcctgca gaaaggtgca gactggagaa atccagttgt tgaaactctt
    18241 tattgttcag caacgcgttg aacaaggtgt ttacgccggt gatagcggta aacggatatt
    18301 tcgccagctc tttgaccagc cccggaatat cccgcgggtt agtgataagc aggttctgcc
    18361 cacccagttc gataaacagc aggcagttaa tggtcagtgc aaaaatgtga tacagcggca
    18421 gcgccgtcac caccagctct ttgcccggat gcagcagagg cccatacgta gcgttaactt
    18481 gttccaggtt cgccagcata ttgcggtgag tcagcatcgc gcctttcgcc acacctgtgg
    18541 tgccgccagt gtattgcagg aaggctaaat cttctggcac cagttcaggt ttgacatact
    18601 gcatacggta gccgttgtgc agggcgctac gaaatgaaat ggcatctggc agatgatatt
    18661 tcggcaccag acgtttgatg tatttaacaa cgaagttaac cagtgtgcct ttcgctgtgg
    18721 aaagctgatc gcccatacgg gtcaggataa cgtgctgaac tgcggtttta tcgaccactt
    18781 tttccagcgt gtgagcaaag ttggacacaa tgacaatcgc cgatgcgccg ctatcgttaa
    18841 gctgatgctc aagctcacgc ggggtataca acgggttaac gtttacgacg atcatcccgg
    18901 cacgcaaaat gccaaacagc gccaccggat attgcaataa attaggcatc atcaacgcaa
    18961 cgcgatcgcc tttcttcagc cccaaccctt gttgcaaata agcggcaaac gcgcgactgc
    19021 gttcttccag cttgcggaag gtcatcacct cccccatatt cacaaacgca ggctgatcgg
    19081 cgtagcgcgc gaccgactgc tcaaacatat ctaccagaga ttgataacgg tcagggttga
    19141 tctccgtcgg aacgtccgcg ggataacggt taagccaaac cttcttcaat tcttcacctc
    19201 taaaatgcgt gttcgtcgtc atcgcaaccc caaatgatat acatgccgtt aacataatat
    19261 taactcatca taccagcttg ataattaccc aacgaaaagg ttgcgaagcg cgtcactatt
    19321 tatttttatc tttaccgtaa gaatgcagaa acagcggacc agccgctgtt tctttttctt
    19381 taaaaacaag caattattca gttacaactg tttgtactct cgcggggccg ggattatacc
    19441 agccccatcc gccgtggcca tagggccagc cacgaccgcc ataaaaccag ggatcaatcg
    19501 gctgaggcgg cataatcacc tgctgggtta aatgccaacg tttgtaacct gttgcctgca
    19561 tcaccataaa tttatagggc gtgttgccga tttttccgtc aaccgcacca gtgattggcc
    19621 cgactaccgt aaccagttgt ccacggaaat ccaccgggtc cagaaaaccg ttcacatcgg
    19681 cataaatgcg accgcgagaa ggttctccca gcgtcggtct ggctccgctg tccagcggta
    19741 cggtagcaat ttccaggcgg gttttccctt gctggttttg taccgcaacc actttgccac
    19801 caaagcgtgc ctcctgacca acgtacagct gcggcgcact catcacccga actaaatctt
    19861 gttgcggcgt gggactgctg cctttaatgg cgtccggcac agtgacacaa ccgctcagca
    19921 ttagcgcaaa cgtacctgcc agtatgcctt tgataacatt cttttgaacc gccatgatgc
    19981 gactcctttt tttctcaggg catactctta agattcattc tttgcccgga agtttcttcc
    20041 atgcgacgtt gttacgtaaa taaaccggtt cggcatgttc caccgccacc gttttaccct
    20101 cggcaaacat ctgacaagca atcggcagca tatcttctgc agcgggcagt aaaacttctc
    20161 cgtcgcgcaa aaccagtccg ctctctttac cgagatccgg ccaggcttgc cagcctgtac
    20221 ctaccgtcac ccattccccg gaaagctgtt gcattcgttc atgaacgagt tcgggtttga
    20281 gtacggcttc ggtttcttca ccgtgccaga taccgttttc atcacgctga tattcggccc
    20341 agtaaacttc gcccattcgc gcgtcaatgg ctgacagcac gcgtgttgcg ccgtttttgc
    20401 gccacgcccc ttgcgccatc gtcattagcg tggagacgcc aatcatcggt aattccgcgc
    20461 caagcgccag cccttgcgcg atgccaatac caatgcgcac gcctgtaaag ctaccggggc
    20521 cgcgcccgta agccagagcg ttaatatcag tcagggaagt tccgctggtg gtcaggatat
    20581 cctgcaccat cggtaagatt cgttgagtat gttcacgagg gcaaagctca aaatgagcgt
    20641 tgacagtacc gtcgttccac agggcgacag agcaggcctc tgtcgcggta tcgatagcca
    20701 gaattcgcat gggtcttcgt gcttagatca ataaaaaggc gcgcatcata ccatactccg
    20761 taacaaatta cctggaggat ggtatcgcaa ggaaacgaac cgcacgggca atgtcacggg
    20821 tgcgtggcgc gggcggcaga ctggcgagaa acgtcgcgcc gtaaggacgc atcaccagcc
    20881 gattgtcaca aattaccaaa accccgcgat cgtcggcgtc gcgaatcagt cgcccgactc
    20941 cctgcttgag agtaatcacc gcatccggca gttgtacttc atcgaacggg tcgccaccgc
    21001 gcaaacggca atcttccatg cgcgctttta acaatggatc atcaggcgag gtaaacggca
    21061 atttgtcgat aatcaccaat gacaatgtat cgccacgcac gtccaccccc tcccagaaac
    21121 tactggttgc cacaagaagc gcgttaccgg cactgacaaa ttgctgcaac agttgccctt
    21181 tgctggtttc cccctgcaat aatacgggaa gcgtcatggt agcgcggaac tgctcggcca
    21241 gatcgcgcat catggcgtgc gaggtacaaa gcataaaaca gcgaccgttg ttagcttcga
    21301 tgatcgggcg cagcatagcc gccagttgcc gcgcggaacc cggttgattg gtttgcggca
    21361 ggttgcgcgg cacacagagt aacgcctggc ggctgtaatc aaacgggctt ggcaatagca
    21421 acgactccgc ctgttctatc ccaagccgcg aggtgaaatg atgcagatcg tcgtttaccg
    21481 acagcgttgc tgaggtgaag atccagctac cgggcttttg cgccattaac tctttgaatt
    21541 tatccgccac gctgagcggc gtgagagcca gagtaaaatg gcgcgaagtg cattcgtacc
    21601 agtagctgta gcccggctga ttgatctctt ttagccgctt cagccgtgtg cgatacaacg
    21661 tggcgcgctc aaatgccgca tccagcaagg cggaacgccc cagtgacagt ttcgccacgt
    21721 cataacaaag ttccagggtg tcatcgagca gtaaaaatgc ccgctgaatt tgcggattag
    21781 ctaacagctc acgcaggtta ccgcgataac ctggttcacc gagttgcaga cgaaaatcct
    21841 gcgcactctg ggcaagacga tcagcgcact tttgtaactg ctgggtgtct tttaattcgg
    21901 tgcggtaggc gatggtgatg tcttttgcca ggtcgagcag ttgtcgactg gagagtgact
    21961 gaccaaaata ctggctggca atgtccggta gctggtgggc ttcgtcgaag atcatgacgt
    22021 ccgcttccgg gatcaactcg ccaaatccac tctctttaac caccatatcc gccagaaaga
    22081 gatgatggtt taccaccacc acatcggcgt ccatcgcttt tttacgtgct ttgaccacaa
    22141 agcaatcttt atacatcggg cagtcgctgc caagacagtt gtcgttggtg ctggtgacca
    22201 gcggccacgc ctgcgaatct tccgccacgc tgacgcaggt gctgatatca ccatcgactg
    22261 tttgattaga ccaggagcgc agcaggatca catcgcttaa tatttgtacc ggaagatcgc
    22321 cccccgccag cgcctgctgt tcgagacgtt cgaggcagag gtagtttgag cgccctttca
    22381 gcaacgccac gttgcccgtg tatttcaacg cctttgagac cgtcggcaaa tcgcggctat
    22441 agagctgatc ctgcaacgct tttgagccgg tcgagataat gactttcttt ttcgcccgca
    22501 gcgcaggggc caggtaagcg taggttttgc ccgtaccggt tcctgcttcc accaccagcg
    22561 gctggccttt ttctatcgcc tgggtgacgg ctaccgccat ctgtcgctgt ggttctcgcg
    22621 gcttaaagcc tggtatcgct ttcgccagct gaccgtctgg tgcaaaatcg tccgtcacac
    22681 taccccctgt tgatttgaac agggattatg tcaggatgag ggcgctttcg ccagttgaag
    22741 tggtgacggc gacctcacat tgtggcagtc tttgcagcac gaaaatggaa ggataatgag
    22801 gataaaatga tgactatcgt tcgtatcgat gctgaagccc gctggtctga tgtagtaatc
    22861 cacaacaaca cgctctacta cactggtgta ccggaaaacc tcgacgccga tgcctttgag
    22921 caaaccgcca acaccctggc gcagattgac gccgtgctgg aaaaacaggg cagcaataaa
    22981 tcgagcattc tggatgccac cattttcctg gccgataaaa acgacttcgc ggcgatgaat
    23041 aaagcgtggg atgcctgggt tgtcgcgggt catgcgccgg tgcgctgcac ggtacaagcg
    23101 ggtttgatga acccgaagta taaagttgaa attaagattg tggctgcggt gtaagcttta
    23161 tcgaagcaaa ataagtcaga cgataattta tcgataatac tggtcggtta tacat
//
LOCUS       DBFIFI010000060        22886 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.060, whole genome shotgun sequence.
ACCESSION   DBFIFI010000060 DBFIFI010000000
VERSION     DBFIFI010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22886
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.060"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(78..359)
                     /locus_tag="N7B39_002194"
     CDS             complement(78..359)
                     /locus_tag="N7B39_002194"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGU2422175.1"
                     /translation="MSQTILFVCATGIATSTAVTEKVMEYCKEQGLNVNYSQTNVASL
                     PGNTDGVALVVSTTKVPYELDVPVVNGLPIITGIGEEKVLAQIVSILKK"
     gene            complement(356..829)
                     /locus_tag="N7B39_002195"
     CDS             complement(356..829)
                     /locus_tag="N7B39_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGU2422176.1"
                     /translation="MQGIHFQENYIQRLPAGLSVEQIIRQLAQPLVAAELVVPDFADH
                     VLKREATYPTGLPTEPPCVAIPHTDHKHVRHNAIAVGILPEPVEFADMGGDPDPVPVR
                     VIFLLALSESNKQLNALGWIMEMIQDTPFMQALLAMETTEIHTVILNKMKERGEI"
     gene            complement(854..1600)
                     /locus_tag="N7B39_002196"
     CDS             complement(854..1600)
                     /locus_tag="N7B39_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGU2422177.1"
                     /translation="MKSLSKSSQIPLYQQVVEWIRESIYTGDLVEDDRIPSEYQIMDM
                     LDVSRGTVKKAVAQLVKEGVLIQVQGKGTFVKKENVAYPLGEGLLSFAESLESQKIHF
                     TTEVITSRIESANRYVAEKLRITPGQDILYLERLRSIGDEKAMLIENRINIELCPGIA
                     EIDFNQHNLFPTIESLSQRKIRYSESRYAARLIGNERGHFLDISEDAPVLHLEQLVFF
                     SRGLPIEFGNVWLKGNKYYLGTVLQRREVS"
     gene            complement(1799..2200)
                     /gene="nikR"
                     /locus_tag="N7B39_002197"
     CDS             complement(1799..2200)
                     /gene="nikR"
                     /locus_tag="N7B39_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HGU2422178.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(2206..3012)
                     /gene="nikE"
                     /locus_tag="N7B39_002198"
     CDS             complement(2206..3012)
                     /gene="nikE"
                     /locus_tag="N7B39_002198"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HGU2422179.1"
                     /translation="MTLLNVSDLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLSRLLVGLESPSQGNISWRGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLHAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQAVGEKLTFSSDAGRVLQNAILPAFPVRRRATEKV"
     gene            complement(3009..3773)
                     /gene="nikD"
                     /locus_tag="N7B39_002199"
     CDS             complement(3009..3773)
                     /gene="nikD"
                     /locus_tag="N7B39_002199"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HGU2422180.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLKRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGK
                     IVEQGDVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            complement(3773..4606)
                     /gene="nikC"
                     /locus_tag="N7B39_002200"
     CDS             complement(3773..4606)
                     /gene="nikC"
                     /locus_tag="N7B39_002200"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HGU2422181.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(4603..5547)
                     /gene="nikB"
                     /locus_tag="N7B39_002201"
     CDS             complement(4603..5547)
                     /gene="nikB"
                     /locus_tag="N7B39_002201"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HGU2422182.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFATQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFTWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(5547..7121)
                     /gene="nikA"
                     /locus_tag="N7B39_002202"
     CDS             complement(5547..7121)
                     /gene="nikA"
                     /locus_tag="N7B39_002202"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU2422183.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWVLQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLHIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDEAQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(7232..7819)
                     /gene="acpT"
                     /locus_tag="N7B39_002203"
     CDS             complement(7232..7819)
                     /gene="acpT"
                     /locus_tag="N7B39_002203"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HGU2422184.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(7821..9050)
                     /locus_tag="N7B39_002204"
     CDS             complement(7821..9050)
                     /locus_tag="N7B39_002204"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HGU2422185.1"
                     /translation="MTRRVVITGMGGVTAFGENWQDVSARLLAYENAVRKMPEWQVYD
                     GLHTLLGAPVDDFTLPEHYTRKRIRAMGRVSQMSTRASELALEQAGLIGDPILTSGET
                     GIAYGSSTGSTGPVSEFATMLTEKHTNNITGTTYVQMMPHTTAVNTGLFFGLRGRVIP
                     TSSACTSGSQAIGYAWEAIRHGYQTVMVAGGAEELCPSEAAVFDTLFATSQHNDAPKT
                     TPSPFDENRDGLVIGEGAGTLILEELEHAKARGATIYGEIVGFATNCDAAHITQPQRE
                     TMQYCMEQSLKIAGLSAQDIGYISAHGTATDRGDMAESLATATIYGDNVPLSSLKSYF
                     GHTLGACGALEAWMSLQMMREGWFAPTLNLNKPDPNCGALDYIMHEARKVDCEFLQSN
                     NFAFGGINTSIIIKRWP"
     gene            complement(9047..9778)
                     /locus_tag="N7B39_002205"
     CDS             complement(9047..9778)
                     /locus_tag="N7B39_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase FabG2"
                     /protein_id="HGU2422186.1"
                     /translation="MSRSVLVTGASKGIGRAIACQLAADGFNIGVHYHRDATGAQETL
                     NAIVANGGNGRLLSFDVANREQCREVLEHEIAQHGAWYGVVSNAGIARDAAFPALSDD
                     DWDAVIHTNLDSFYNVIQPCIMPMIGARQGGRIITLSSVSGVMGNRGQVNYSAAKAGI
                     IGATKALAIELAKRKITVNCIAPGLIDTGMIEMEESALKEAMSMIPMKRMGQAEEVAG
                     LASYLMSDIAGYVTRQVISINGGML"
     gene            complement(9778..10242)
                     /locus_tag="N7B39_002206"
     CDS             complement(9778..10242)
                     /locus_tag="N7B39_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-fatty acyl-ACP dehydratase"
                     /protein_id="HGU2422187.1"
                     /translation="MSHYLSPGAYLPHDAPMLLLEDVVSVSDDSAVCRVTVSPSGVLA
                     PFLDPDGNLPGWFTLELMAQTVGVWSGWHRHQQGKNSIELGMILGARELLCTTGILPA
                     GQTLTITVKLLMQDDRFGSFECSINDGEATGRINTFQPTAEELTTLFQQGAS"
     gene            complement(10239..11408)
                     /locus_tag="N7B39_002207"
     CDS             complement(10239..11408)
                     /locus_tag="N7B39_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HGU2422188.1"
                     /translation="MIYISAVGMINALGNNLDEIATNLTRGVAPGMRPRAGWLQGHPQ
                     AVLAGVDGELPLIPEKFAAHRSRNNQILLAALAQIQPQVDDAIAKYGRERIAIVLGTS
                     TSGLHEGDTHVNLRTHGQPSTTWHYAQQELGDPSRFLSHWLALDGPAYTLSTACSSSA
                     RAIISGRRLIEAGLVDAAIVGGADTLSRMPINGFHSLESLSPTLCQPFGRDRAGITIG
                     EGAGLMLLTREPQPIALLGVGESSDAYHISAPHPQGEGAIRAINQALTDAQLTPDDVG
                     YINLHGTATQLNDQIESMVVNALFGERVPCSSTKHLTGHTLGTAGITEAAISMLILQR
                     DLPLPAQDFSLSPRDPTLPPCGIIEKPQPLARPVILSNSFAFGGNNASILLGRVS"
     gene            complement(11410..11994)
                     /locus_tag="N7B39_002208"
     CDS             complement(11410..11994)
                     /locus_tag="N7B39_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3261 domain-containing protein"
                     /protein_id="HGU2422189.1"
                     /translation="MIKSTFWRAFALTATLILTGCSHSQPEQEGRPQAWLQPGTRITL
                     PAPGISPAVNSQQLLTGSFNGKTQSLLVMLNADDQKITLAGLSSVGIRLFLVTYDAQG
                     LRAEQSIVVPQLPPASQVLADVMLSHWPISAWQPQLPAGWTLRDNGDKRELRNASGKL
                     VTEITYLNRKGKRVPISIEQHVFKYHITIQYLGD"
     gene            complement(11991..14309)
                     /locus_tag="N7B39_002209"
     CDS             complement(11991..14309)
                     /locus_tag="N7B39_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HGU2422190.1"
                     /translation="MTNANVLPPSKRPALLWGLVCLVMAVALLILLPQSRLNSSVLAM
                     LPKQTMGDIPPALNDGFMQRLDRQLVWLVSPGKEANPLVAQEWLTLLQKSAALGDVKG
                     PMDAASQQAWGAFFWQHRNGLIDPNTRARLQNGGEAQAQWILSQLYSAFSGVSGKELQ
                     NDPLMLMRGSQLAMAKNGQRLRLMDGWLVTQDPQGNYWYLLHGELAGSSFDMQQTHQL
                     ITTLNTLEKDLKTRYPQAQLLSRGTVFYSDYASQQAKQDISTLGVATLLGVILLIVAV
                     FRSLRPLLLCVISIGIGALAGTVATLLIFGELHLMTLVMSMSVIGISADYTLYYLTER
                     MVHGNDVSPWQSLAKVRNALLLALLTTVAAYLIMMLAPFPGIRQMAIFAAVGLSASCL
                     TVLFWHPWLCRGLPVRPVPAMALMLRWLAAWRRNKKLSLGLPVALALFSLAGMSMLRV
                     DDDISQLQALPQHILAQEKAITALTGQSVDQKWFVVYGDSPQQTLRRLEKYTASLEYA
                     KKEGLISNYRTIPLNSLARQEEDLQLLKTAAPTVTKALQNAGLTAVNPDLNAMPVNVD
                     EWLASPASEGWRLLWLTLENGESGVLVPVEGVKSSALMQEIATYYPCGIAWVDRKSTF
                     DELFALYRYVLTGLLLVALAVIACGAVARLGWRKGLISLVPSVLSLGCGLAVLAMSGQ
                     AVNLFSLLALVLVLGIGINYTLFFSNPRGTPLTSLLAIALAMLTTLLTLGMLVFSATQ
                     AISSFGIVLVSGIFTAFLLSPLAMPDKKRTKK"
     gene            complement(14278..14883)
                     /locus_tag="N7B39_002210"
     CDS             complement(14278..14883)
                     /locus_tag="N7B39_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HGU2422191.1"
                     /translation="MKFLPLLALLISPFVSALTLDDLQQRFTEQPVIRAHFDQTRTIK
                     DLPQPLRSQGQMLIARDQGLLWDQTSPFPMQLLLDDKRMVQVINGQPPQIITAENNPQ
                     MFQFNHLLRALFQADRKVLEQNFRVEFADKGEGRWTLRLTPTTTPLDKIFNTIDLAGK
                     TYLESIQLNDKQGDRTDIALTQHQLTPAQLTDDERQRFAAQ"
     gene            complement(14880..15302)
                     /locus_tag="N7B39_002211"
     CDS             complement(14880..15302)
                     /locus_tag="N7B39_002211"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HGU2422192.1"
                     /translation="MLNDPRFTAEVELTIPFHDVDMMGVAWHGNYFRYFEVAREALLN
                     QFNYGYRQMKESGYLWPVVDARVKYRHALTFEQRIRVRAHIEEFENRLRIGYQIFDAE
                     TGKRATTGYTIQVAVDEQSRELCFVSPDILFERMGVKP"
     gene            complement(15306..16982)
                     /locus_tag="N7B39_002212"
     CDS             complement(15306..16982)
                     /locus_tag="N7B39_002212"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGU2422193.1"
                     /translation="MSVNFSPCVLIPCYNHGAMMPGVLARLKPFNLPCIVVDDGSDAT
                     TQQQLDSLLAEQPGVTLIRLAENAGKGAAVMRGLQAAADAGFSHAVQVDADGQHAIED
                     IPKLLALAEQQPAALISGQPIYDDSIPRSRLYGRWVTHVWVWIETLSLQLKDSMCGFR
                     VYPVAPTLQLAKHATIGKRMDFDTEVMVRLYWQGNTSYFVPTRVTYPLDGLSHFDALK
                     DNVRISLMHTRLFFGMLPRIPSLLMRRSSCHWARQSEVKGLWGMRLMLLVWRLLGRTA
                     FSALLYPVVGVYWLTASRARKASQDWLARVRQHQPQAAKLNSYQHFLRFGNAMLDKIA
                     SWRGELQPGRDVLFAPGAEAALDVRDPRGKLLLASHLGDVEVCRALAKIQGYKTINAL
                     VFSENAQRFKQIMQEMAPQAGINLMPVTDIGPETAILLKEKLDNGEWVAIVGDRIAVN
                     PQRGGDWRVCWSSFMGQPAPFPQGPFILASILRCPVNLIFALRQHGKLHIHCESFADP
                     LQLPRGERQQALQNAIDHYAARLEHYALQSPLDWFNFFDFWQLPEIQDKE"
     gene            complement(16973..17326)
                     /locus_tag="N7B39_002213"
     CDS             complement(16973..17326)
                     /locus_tag="N7B39_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymyristoyl-ACP dehydratase"
                     /protein_id="HGU2422194.1"
                     /translation="MKPHEIERHQAQTNHLEIVLHLRADLFWFRGHFAVQPLLPGVAQ
                     IDWAMSYALNLLAPGWRFHSIQNIKFQSPLLPENRVTLALNWQEERQILSFSYQRHDG
                     DARHTASSGKIRLCR"
     gene            complement(17313..18674)
                     /locus_tag="N7B39_002214"
                     /pseudo
     CDS             complement(17313..18674)
                     /locus_tag="N7B39_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312358.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase"
     gene            complement(18671..19252)
                     /locus_tag="N7B39_002215"
     CDS             complement(18671..19252)
                     /locus_tag="N7B39_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2422195.1"
                     /translation="MSGIRSLPMIKLLTGLLLLAWPFVIWFGLAHNGLHWLLPLMALL
                     LLLRLRQTRRQAGPLQAVTQLVAVVGIALCVASFMLKTHQLLLFYPVVVNAVMLAVFG
                     GSLWSAMPIVERLARLQEPDLPEKGVRYTRHVTQIWCGFFIINGGIALFTALYADMSL
                     WTAWNGMIAYLLMGTLMAGEWLLRRQVMKRDRA"
     gene            complement(19257..19508)
                     /locus_tag="N7B39_002216"
     CDS             complement(19257..19508)
                     /locus_tag="N7B39_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HGU2422196.1"
                     /translation="MTEQQTVYQEVSALLVKLFEIDPQDIKPETRLYEDLELDSIDAV
                     DMIVHLQKKTGKKIKPEEFKAVRTVQDVVEAVERLLQEA"
     gene            complement(19520..19777)
                     /locus_tag="N7B39_002217"
     CDS             complement(19520..19777)
                     /locus_tag="N7B39_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HGU2422197.1"
                     /translation="MQALYLEIKNLIISTLNLDELTPDDIDTDAPLFGDGLGLDSIDA
                     LELGLAVKNEYGIVLSAESEEMRQHFFSVATLASFIAAQRA"
     gene            complement(19752..20573)
                     /locus_tag="N7B39_002218"
                     /pseudo
     CDS             complement(19752..20573)
                     /locus_tag="N7B39_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312354.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
     gene            complement(20570..21292)
                     /locus_tag="N7B39_002219"
     CDS             complement(20570..21292)
                     /locus_tag="N7B39_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HGU2422198.1"
                     /translation="MKFALNILDWQARAPGLSETLQWQEWSRQPQAIDPTAPLAKLSE
                     LPMMTARRLSSGSKLAVECGLAMLRRHQPDAVLYTSRHGELERNYRIVHALATEQALS
                     PTDFALSVHNSSVGNLTIAAKQPSVSSSLSAGRDSFQQGLCEVLSLLQAGYQRVLMVD
                     FDGFLPEFYHPQLPSEMPTWPYAVALVIEAGDDWQCETQSAIAGNETTLPQSMLFLQH
                     YLQNVDTFSLPGERVQWRWSHR"
     gene            complement(21333..22391)
                     /locus_tag="N7B39_002220"
     CDS             complement(21333..22391)
                     /locus_tag="N7B39_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HGU2422199.1"
                     /translation="MYEQDSLSALDAITEAQRIAFAPMLFQTALCLRNAGVLSYLDRQ
                     GKHGATLADITEHSHINEYATSVLLDMGLSGRIITCKEGIYYLAKIGHYLLHDTMTRV
                     NMDFTQDVCYQGLFFLADSLNEGKPSGLKVFGDWSTIYPALSQLPDAARESWFAFDHY
                     YSDGAFNAALPYVFANNPTTLYDVGGNTGKWALRCCKYNENIAVTLLDLPQQIVLAKE
                     NIANAGFSDRIDFHAVDMLSDAPLPGEADIWWMSQFLDCFSPEQIISILSKIASVMKP
                     GAKVCIMELFWDAQRFEAASFSLNASSLYFTCMANGNSRFYSAEKFYDYLNKAGFQVA
                     ERHDNLGVGHTLLICQKK"
     gene            complement(22460..22837)
                     /locus_tag="N7B39_002221"
     CDS             complement(22460..22837)
                     /locus_tag="N7B39_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGU2422200.1"
                     /translation="MKWFAAIAVVGALAGCARTAPIDQVHSTVSAGHTQEQVKKAILK
                     AGVERKWIMSEAGQGIIKARQQSRDHSAEIRINYTASSYDINYENSQNLQASGGQIHK
                     NYNRWVRNLDKDIQLNLSAGADL"
BASE COUNT         5229 a         6244 c         6288 g         5125 t
ORIGIN      
        1 taataaacct ctttttcagg cgagagcgca cctcgcgtta gcgcctgatg gagtgggttg
       61 aaattaatta ttattaatta ctttttaagg atagaaacaa tttgcgccag tactttttct
      121 tcgccaatac cggtaataat cggcaagccg ttcaccaccg ggacgtcgag ttcataaggc
      181 actttagttg ttgaaactac cagtgctacg ccgtcagtat ttccaggtaa agaggcaacg
      241 ttggtttggg aataattgac gttaagacct tgctctttgc aatattccat gactttttct
      301 gttaccgccg tggatgtggc aatacccgtg gcgcagacaa ataaaatggt ttgactcata
      361 tttcacctcg ttctttcatt ttgtttaaga ttactgtgtg tatttctgtt gtttccatcg
      421 caagcagggc ttgcataaag ggcgtatcct gaatcatttc cataatccag cccagggcat
      481 ttaattgttt attgctttcg cttaaggcga gcaaaaaaat caccctgaca ggtacggggt
      541 caggatcgcc gcccatatcg gcaaactcta ccggttccgg caggatgccg acggcaattg
      601 cattgtgcct gacatgttta tggtccgtgt gcggaatagc gacgcacggt ggttcggtag
      661 gcagccccgt tgggtacgtc gcctcacgtt tcagtacgtg atcggcaaaa tcagggacga
      721 ccagctcggc ggcaaccaac ggttgcgcta actggcggat gatctgttca acgcttaacc
      781 ctgccggaag tcgttgaata taattttcct gaaaatgtat gccttgcatt ttattcctct
      841 taaaaaagtg tacttaactc acttcccgcc gttgcagtac ggtgccaaga taatatttat
      901 ttccttttaa ccagacgttg ccaaactcaa tgggtaatcc tcgggagaaa aagactaact
      961 gctccagatg caaaacgggt gcgtcttcac tgatatctaa aaaatgacca cgttcattac
     1021 caattaatcg cgcggcatag cgactttccg agtaacgaat ttttctttgc gacaaacttt
     1081 ctattgttgg aaataaattg tgttgattaa aatcgatttc agcaataccg gggcatagct
     1141 caatattgat acggttctct atcagcatcg ctttttcatc accaattgag cgtaagcgtt
     1201 caaggtaaag aatatcctga ccgggcgtta ttcttaattt ctctgccaca taacgattag
     1261 ccgattcaat ccgtgacgta ataacttcag tggtaaaatg tattttctgg ctttccagcg
     1321 attccgcgaa tgacaataat ccttcaccta acggatatgc cacgttctct tttttgacaa
     1381 atgttccctt accctggacc tgtattaaca cgccttcttt taccagttgg gcgaccgctt
     1441 ttttaacggt tccccgactc acgtccagca tatccataat ctggtattcc gaaggaatgc
     1501 gatcgtcttc caccagatct ccggtataaa tactttctct tatccactcc accacttgtt
     1561 gatagagcgg tatttgtgac gacttactta atgatttcat ggcgcgcacc tgaacattta
     1621 cagttgtcta tggacaatca tggacaactt caagcgtata gtaggtgaac gggagaaaaa
     1681 gaggatcgaa gcgtctcaca ttttgaggga ccaaatcgtg atcaaaaaaa tgtgacgtga
     1741 gtcgatattt gtcagaagtt tactgcgccg taaggaaata acggcgcgtt ggtaggtgtt
     1801 aatcttcctt cggcaggcac tgcaaatgcc cgtgccgcac gccgcgctgg gcgataacgt
     1861 catcggcaaa atgctgcacg tcacccatgt cacctttcaa cacggcgatt tccagacagt
     1921 cgtcgtggtt gatgtgcaca tgtagcgtgg cgacggagag gtcgtggtga tgatgctggg
     1981 tggaaacaat acggctggct aagtcgcgtt tttcgtgttc atacacatac gacagcaccg
     2041 cgaaaccttg tgtgccgtgc tgctgggtag cttcctgcgc cagggcgcta cgcagaatat
     2101 cgcggatagc ttcggaacgg ttgttataac cacgacgctg gctcaggctg tccagcgtct
     2161 ccagtaaatc gtcatcaagc gtgatggtga ctcgttgcat ttgcgttaaa ccttttctgt
     2221 ggcgcgacgg cgcacgggga atgcgggtaa tatcgcgttt tgtagcacac gtccggcgtc
     2281 agaggaaaag gttaatttct ctcccaccgc ctgggtttcg acgatttgtc cgttgtccat
     2341 caccatcact cgctggcaaa agcgttccac caggcgtaag tcatgagtga tgaacaggca
     2401 ggcagtgcca aactgttgtt gtagcttttt cagcaggcga atgacgcccg cctgtaacac
     2461 gagatcaagg ttagaaacgg cttcatccag aatcagcaat ttcggttcga ccgccagcgc
     2521 gcgtgccagg cagacgcgct ggagctggcc gccgcttaac tgcggtgggc gcttgtcgag
     2581 cacgctgtcg tcgagatcga ccgcgtgcag catttcgctg gcacgcgcca gttgttcgga
     2641 ttttttcagc gatagcaggt ggcgcatcgg ttcccgcagg atctcgcgca cggttttgcg
     2701 cggattcacg gcgctgatgg agtcctgaaa taccatctga atatcgcggc ggaacgcttt
     2761 acgctgggcg cgattgagct ttgccaacga ttcgccacgc cagctaatat ttccctgtga
     2821 gggcgattct aagcctacca gtagccgcga gagagtgctt tttccgcagc cgctgcgtcc
     2881 cagcagggcg acggtttcac cgcttttcag ggtcagggaa acgttattca gcaccgcctg
     2941 gtgctgatgt tttccgctaa acccaccgtg ggcatagtga tgggaaaggt cggagacgtt
     3001 aagtaaagtc atgatgccag ctccataccg tagagggcga gatgagcgga aaccaggctg
     3061 cgcgttaccg catgtttggg ggcgttaaac agcgtttcga catcgccctg ttcgacaatc
     3121 ttaccgtcag acattaccgc tacgtcatcc gccagacgcg ccaccacgcc catatcatgg
     3181 gtgaccagca gcatccccgg cgcctgtttt tgcataatgc tttccagcag atcgagaatg
     3241 cgtgcctgtg ctaccacgtc gaggtcggtg gttggttcat cggcgatgat aaacggtgat
     3301 tcgcaaagca ccgccatcgc aatcatcatg cgctgcaaca tgccgccgct catctcgaac
     3361 gggtacagct tcagcacgcg tgcggcgttt tccagcccca ccgcttctat ggcagcggta
     3421 agcgtagcgt catcggcggg tttccccagc gccaggcacg tctcccgcgc gtgggtgtgc
     3481 atggtgtgca gtggattaaa ggcgctgcgc gggttctgca tgatggtggc aattttgatg
     3541 ccgcgcaggg cgcagggaga aaccggtttg ccatcggcta aaatttcccc cgccgtctgg
     3601 cgaacgcctg cgggcagaat tcccagcgtc gcggcgcagg ttaatgattt cccgctgccg
     3661 ctaccgccga ctaacgccag cacgcgcccg cgtttcaggg ttaacgacac gccgtgcacc
     3721 agcggctgcg cggcctgtag cgcgatatta cgtagttcaa tctgttgcgg cattagtgtg
     3781 cgtgctccgt caccagatga ggatccagat gatcgcgcag tgcgtcaccc accaggttaa
     3841 aggccatcac gctgataaac agcgccagcc ccggccagaa catttgcagc ggctgggtcc
     3901 agatatactg gcgcgcgtcg ttaatcatca cgccccattc ggcggtcggc gcggtcacgc
     3961 cgaggccgag gaaggacatc ccggcaacgt gcagcatcat atggccgata tccagcgttg
     4021 ccagcaccag cagcgaaggg atcaccgcac ctgccagatg atcaacaaac actcgcacat
     4081 ggcccgcgcc agaaagccgt gatgccagca caaactcgcg ctggcgcagt gaaatcacca
     4141 ggctgcgcac catgcgtgca taccacgccc agtgcgacag ggcgatggcg ataattacgt
     4201 tggtcagccc agtgccgagc acgccaacca taaagaatga cagaatcgag gtcgggaagg
     4261 tcataaacat atcggcgacg cgcatggtag cctgatcaac gcgcccgcca atcagcccgg
     4321 cgctgccgcc aataaccagc cctaatgtca gcaccagcag caggcaggcc attaccgaac
     4381 cgagcgacac acgggtcgct gccatcagcc gcgagaaaat atcgcgccct aagtgatcgg
     4441 tgcccaacca gtgctgcgca tccggcgaaa gcaggcgcga cggcaaatca atcgcctggg
     4501 ggtcatacgg cagccaccac tggctggtga gcgcaatcag cgccagcagg gcgataatga
     4561 tcagcgccag gcgtaccgac cagcgggaag agaggaaaaa gttcacgcgt gcgctccttc
     4621 atgacgacga atgcgtgggt ccagtgcggc gttgagcaaa tcgacaatca aattacagac
     4681 cacaaaaacc accaccatca tcagcgtaaa gcactggatc accggatagt cacggttaaa
     4741 aatcgccgac accgcatagc gcccgacgcc gggccaggta aagatgtttt cgataatcat
     4801 cgtcccgcca atcagttcgc caatgtgcat ccccacggcg gtgatcatcg gcagcgaggc
     4861 attgcgcaga atatgacgac gctcggtctg tttgtcattc aggccgcgta gccgcgccca
     4921 ggtgacgtga cgctgaccgg cgacgtccag catactggcg cgcagcagcc gtgcgttaat
     4981 cgccagcgac ataaaggcaa tggaaaccgc aggcaaaatg atgtgctgcc agccgccgta
     5041 gcccatcgcg ggtagccatt gcagatacac cgaaaacgcc atcaccagta aaaatgccag
     5101 ccagaagttg ggcatcgaca cgccgagaaa cgcgatgaaa cgtacggcga aatccggcag
     5161 acggtcacga tggcgcgccg cccagatacc gagcggtacg gaagtgagca gaattaatac
     5221 cagcgccgca cccgccagtt ccagcgtggc gggcaggaag ttcagcatat cgtccagcac
     5281 cgggcgctgc gtggcaaatg agatgccgaa atcaagatgc agcgccttcc acaaccaggt
     5341 gccgtactgc acatacagcg gctgatccag tcccagcatg gtgcgggtag aggccagcat
     5401 ctccggcgtc ggcggcaggt tagacagacg caaataatcg agcgccgggt cgccggtacc
     5461 gaggcgcagc atcagaaaaa tgatcaccga ggcggcgagc accatcggga tcagcagcag
     5521 aaagcggcgt aatacgtaac gcaacattaa ggtttcaccg gtttaatctg ttcgaacgga
     5581 atttcggtgg cgatcggcgc gtaggggatg ttacccagct ccggttttga taccaccatc
     5641 attgagatgt aactgatagg cagataaacc gcctcgtcat gcagacgggt cagaatgtcg
     5701 cgatacagtg cctgacgttg tgcttcgtca tgggtcgcca gcacttcgcc gatctcttta
     5761 tcaatcagcg gcttgtcggc taatccttgc tgtgcctgga agtcagcgtg cgacggtacg
     5821 cgcattgaac tcaggaaggc gtgtggatcg tatggcgcgc cccaggtgcg gtggaaaatc
     5881 atgccaaaac gaccgtcgcg ctgacgcgca tagatactgc tctcttcttc gccaatcagc
     5941 gagacatctg cgccaatctg gcgcatatca gcctgaatga tttccgccat cgatttgctt
     6001 aacgcatcgg tgccgatgaa cgaaagttca atgtgcagcg gctgaccatt tttctcgcgg
     6061 atgtctttgc ccgcaggcag cgtccaaccg gctttttcca gcaacgcttt cgctttctgc
     6121 ggatcgtact ggcgcggttt caggccgagg ttggcgtagg gcacagaagg ggcaaacagg
     6181 gtgtcggcga cctgctgggt gccatacaac gcgttatcaa tcagcgattt tttgtttacc
     6241 gcgtaattaa gagcttcacg tactgccagc tcattggtgg gggctttggc ggtattaagc
     6301 gccagcatca cggtttcgat cggctgtgac agttgggtat ggtaagccgg gttctggcta
     6361 aagcgggcga aggtatcgag cggtaataac ccttcgtttc cgtacagcag gtcgatatcg
     6421 ccagtttcaa acgccaccgc gcgggtagtc gggtctggga tgacgttaaa agtgatcttc
     6481 ttaattgccg gcttttcgcc ccagtagttt tcgttacgga cgaagacatc gtactgattc
     6541 agtttcgatt cctgcaaaac ccacggtccg gtgccaatcg gcgctttaat cccgttcatg
     6601 gtttcatggt ttttaaactg cgagggggcg ataaagcgga aagggcgcgg cagggccagt
     6661 tcttgcagga aaggatagta ggcgcttttc agggtgattt gcagctctgt tttactgagt
     6721 gctttaacat caacaatctg gtttgccagc tccagccagg cgtgacgttg acggttatcg
     6781 agcactgcgc ggaagttttc tgccgccgcc tctgcatcaa acggttcgcc gttagagaat
     6841 ttcacgtcat cacgcagggt gaaggtccag gttttaccat cttctgaatg agtccagctt
     6901 tttgccagcc acgggatcac cgaaccgtct gcctgatatt tcaccaatgg ttcataaacc
     6961 atgctctggg cgaacatctg gttaggcgtg taaaggtgcg ggtttagtgg cccgacattc
     7021 accggccagg cggtggtgat ttcatctggt gcagcggcat ggacgataaa agacgcacaa
     7081 gccagcagcg caaatagagt gcggcggagt gtggagagca tggtaacccc aatggattaa
     7141 aatagatggc ggaaataagt atgacgattt taagtattcg tcatactgat tacctgttct
     7201 ggatcaatag atgggcagtc gggtgggcgg atcagttaac tgaatcgatc cattgcacac
     7261 tgtcggcggt gagcgtaaag ggagtagggg tgcagatcgc caggcttaaa ttttctagct
     7321 gacaatggct gactgacagc gaggagtgat aggtgctgtc tacgctgacg atttgccagg
     7381 cgctgccacc acgctgttta acgatggctt ctttgcgcgt ccagatgcgc cagaacattt
     7441 ccagttgctg atcaggatgc acggcgtcca tctcagcatg ttccccgagg ctgaacacgg
     7501 cgttcgccag ccagcgccag ttggcgcgcg ggcgaatcac ttcgatatcg cagccgactt
     7561 cgccttcatc actcaacagc agggcgatat cgtcaccgct atggcttaag ttgaaccata
     7621 gcggcgtttc cggcgcaaat gcaggtttgc cttgttcgcc atagatgatc tccggtagcg
     7681 gggaaagcgt gtgcgaaagc aatgcacgcc ccgccagcca gcgttcgcgt cgtggacctt
     7741 gcggtgcttg ctcgcgtaaa cccggtggca gtggagctgc gcttaaggtc gaaactttcc
     7801 ccagaactat ccgatacata tcagggccaa cgtttaatga tgatggatgt attaatgccg
     7861 ccaaaggcaa agttgttact ctgcaaaaac tcacaatcaa ccttgcgggc ttcatgcatg
     7921 atgtaatcca gcgcgccaca attcgggtct ggtttattca ggtttagcgt aggcgcaaac
     7981 caaccttcgc gcatcatttg cagactcatc caggcttcga gcgcgccaca ggcaccaagc
     8041 gtatgaccaa aataactttt cagcgaggag agcggcacat tatcaccgta aatggttgct
     8101 gtcgccagac tttctgccat atcaccgcga tcggtcgccg taccatgcgc tgaaatatag
     8161 ccgatatctt gtgcgcttaa cccggcgatt ttcagcgatt gctccataca atactgcatg
     8221 gtttcgcgct gcggctgggt aatatgtgcc gcgtcgcagt tagtcgcaaa gccgacgatt
     8281 tcaccgtaaa tggtcgcgcc gcgcgctttg gcgtgctcca gttcttcgag aatgagcgta
     8341 cctgcgcctt caccaatcac cagcccgtcg cggttttcat cgaacggtga aggtgtggtt
     8401 ttcggcgcat cattgtgctg gctggtggcg aaaagcgtat caaataccgc tgcttctgaa
     8461 ggacataact cttcggcacc accagcaacc atcaccgttt gataaccgtg gcgaatggct
     8521 tcccacgcat aaccgattgc ctggctgccg gaagtacagg cgctggaggt tgggatcacc
     8581 cgcccgcgca ggccgaaaaa gaggccagta ttaacggcgg tggtgtgcgg catcatctgc
     8641 acataggtgg tgccagtgat gttattggtg tgcttttccg tcagcatggt ggcgaactcg
     8701 cttaccgggc cggtactgcc ggtcgatgaa ccgtacgcaa tgcccgtttc gccgctggtt
     8761 aatatcggat cgccaatcaa ccctgcctgc tctaacgcca gctcgctggc acgggtcgac
     8821 atttgcgaca cgcggcccat cgcacggata cgcttgcggg tgtagtgctc cggtagcgtg
     8881 aaatcatcaa caggcgcgcc aagcagggtg tgcagcccat cgtagacctg ccactccggc
     8941 attttgcgca ccgcattttc atatgccagt aagcgtgcag aaacgtcctg ccagttttca
     9001 ccaaaggcag tcacgccgcc cattcctgta atcactacgc gacgtgtcat aacatccctc
     9061 cgttgatgga aataacctgg cgggtgacgt aacccgcaat atcagacatt aaatagctgg
     9121 caagcccggc aacttcttcc gcctggccca tgcgtttcat ggggatcatc gacattgcct
     9181 ctttcagggc gctctcttcc atttcgatca tcccggtatc aataagcccc ggcgcgatgc
     9241 agttgacggt gattttgcgc ttcgcgagtt cgatagccag cgcttttgtt gcgccgataa
     9301 ttccggcttt ggcagcgctg taattcacct ggccgcgatt gcccattacg ccggaaaccg
     9361 aggatagcgt gatgatgcgc ccaccctggc gtgcgccgat catcggcata atgcacggct
     9421 gaatgacgtt gtagaaactg tcgaggttgg tgtggatcac cgcatcccag tcgtcatcgc
     9481 ttaaagccgg aaaagcggca tcgcgggcga tcccggcgtt actgaccacg ccgtaccagg
     9541 caccgtgttg cgcgatttcg tgctccagca cttcccgaca ctgttcgcgg tttgcgacgt
     9601 cgaaactgag caaacgcccg ttacccccat tggcgacaat ggcattcagc gtttcctgag
     9661 cgcctgtggc gtcacggtga tagtgaacgc caatgttaaa gccgtctgcc gccagttggc
     9721 aggcgatggc gcgaccaatg cctttgctgg caccagtaac cagaactgaa cgactcatca
     9781 ggacgctcct tgttgaaaca gggtggtaag ttcttccgcg gtcggctgga aggtgttaat
     9841 gcggcccgtc gcctcgccgt cgttgatgct gcactcaaaa ctgccgaagc gatcgtcttg
     9901 catcagcagt ttgacggtaa tggtcagtgt ctggcctgcg ggcagaatgc cagtggtgca
     9961 aagcaattca cgcgcgccca gtatcatccc taactcaata ctgtttttgc cctgctggtg
    10021 gcgatgccag ccagaccaga cgccgacggt ttgcgccatc agttcaaggg taaaccagcc
    10081 ggggagattg ccgtccgggt cgagaaacgg tgccagtacg ccactgggcg aaaccgtcac
    10141 gcggcacacc gcgctgtcat cgctgacgct caccacgtct tccagcagta acatgggcgc
    10201 gtcgtggggc agataagcgc cgggggataa atagtggctc acgaaaccct cccgagcaga
    10261 atgctggcgt tattgccgcc aaaggcgaag gaattggaca ggatcaccgg acgtgccagc
    10321 ggctgcggtt tttcgataat gccacacggt ggcagcgtgg gatcgcgcgg cgacaggctg
    10381 aagtcctgag cgggcagcgg taaatcacgc tgtaaaatca acatactaat tgccgcctcg
    10441 gtaatgcccg ctgtgcccag cgtgtgtccg gtcaaatgtt tggtggagct gcacggtacg
    10501 cgttcgccaa acagggcgtt aaccaccatt gattcaatct gatcgttgag ttgtgtggcg
    10561 gtgccgtgca ggttgatata gccgacgtca tctggcgtaa gctgcgcatc ggtcagcgcc
    10621 tgattaatag cgcggatcgc tccttcgccc tgcggatgcg gggctgaaat atggtaagcg
    10681 tcgctcgatt caccaacgcc cagcaacgcg atgggctgcg gttcccgggt cagcagcatt
    10741 agccccgccc cttcaccaat ggtgatacct gcgcggtcac gaccaaacgg ctgacataag
    10801 gttggcgaca gcgattcgag gctgtgaaaa ccgttaatcg gcatccggct cagggtgtct
    10861 gcgccaccca caatggcggc atcgaccagc cccgcttcga taagcctgcg cccgctgatt
    10921 atcgctctgg cgctggaaga gcaggcggtt gaaagggtat aggccgggcc gtcgagcgcc
    10981 agccagtggc tgagaaaacg cgacggatcg ccgagttcct gttgcgcgta gtgccatgtg
    11041 gtgctcggct gcccgtgggt gcgcaggtta acgtgcgtgt cgccttcatg caggccggag
    11101 gtgctggtac caagcacaat cgcaatgcgc tcgcggccat atttcgcaat ggcgtcatca
    11161 acctgcggct ggatctgcgc cagcgccgcc agcaggatct gattgttgcg cgagcggtgc
    11221 gcagcgaatt tttccgggat aagcggaagc tcgccatcca cgccagccag gaccgcctgc
    11281 ggatgcccct gcaaccagcc tgcgcgcggg cgcatgccgg gagcaacacc gcgagtcagg
    11341 ttggttgcga tttcgtcaag gttatttccc agggcgttga tcatgccgac ggcggaaata
    11401 taaatcatat cagtcaccta agtattgaat ggtgatgtgg tatttaaaga catgctgctc
    11461 aatgctgatt ggcacgcgtt tgcccttgcg attcagatag gtgatttccg tgaccagttt
    11521 gccgctggcg ttacgcagct cgcgtttgtc accgttgtcg cgaagtgtcc atcctgcggg
    11581 aagttgcggt tgccaggcgc taatcggcca gtggctgagc atcacgtcag ccagtacctg
    11641 acttgcgggc ggtaactgtg ggacgacgat ggattgctcg gcgcgtagcc cttgcgcatc
    11701 gtaggtcacc agaaacaggc gaatgccgac cgacgacagc ccggcaaggg tgattttctg
    11761 atcgtcggcg ttaagcatca ctaacagcga ctgggttttg ccgttgaagc tgccggtcaa
    11821 cagttgctgc gaattgactg cgggtgaaat ccctggtgcc ggcagcgtga tgcgcgtgcc
    11881 aggttgcagc cacgcctgcg ggcggccttc ctgttctggt tgcgagtggc tacagccggt
    11941 gaggataagc gtagcggtca gggcgaacgc tcgccagaat gtggatttga tcattttttt
    12001 gttctctttt tatcgggcat agccagcggc gaaagcagga aggcggtgaa aataccgctc
    12061 accagcacaa tgccaaaact gctgatggcc tgggtggcgc tgaataccag catacccagc
    12121 gtcagcaagg tggtgagcat tgccagcgcg atcgccagta gcgaagttaa cggtgtaccg
    12181 cgcggattac tgaaaaacag cgtgtagttg ataccgatgc caagcaccag caccagcgcc
    12241 agcagcgaaa agagattcac cgcctgcccg ctcatcgcca gcaccgccag accacagccc
    12301 agcgaaagca ccgaaggcac caggctgata agccctttgc gccagccgag acgggccact
    12361 gcgccgcagg caatcactgc cagcgccacc agcaacaagc cggttaagac gtagcggtaa
    12421 agtgcgaaca attcatcaaa ggtgcttttg cgatcaaccc aggcaatgcc gcaagggtaa
    12481 tatgtggcga tttcctgcat caacgcgcta cttttaaccc cttcaaccgg caccagtacg
    12541 ccgctttcgc cgttttccag cgtcagccac agcagacgcc agccttcact ggcggggctt
    12601 gccagccatt catcaacgtt cactggcatg gcgttgagat ccgggttcac tgccgtcagc
    12661 ccggcatttt gcagcgcttt tgttactgtc ggggccgccg ttttcagcag ttgtaaatct
    12721 tcctcctgcc gcgcaaggga gttcagcgga atggtgcggt agttgctgat aagcccctct
    12781 tttttcgcat actcaagtga ggcggtatat ttctccagtc gccgcaatgt ttgctgtggc
    12841 gaatcgccgt aaaccacaaa ccatttttga tcgacgctct gcccggtcag ggcggtaatg
    12901 gctttttcct gcgccagaat atgctgcggt agcgcctgta actgcgagat atcgtcatcg
    12961 acgcgtagca ttgacatccc cgccagcgaa aacagcgcca gcgcgacggg cagacccagc
    13021 gacagttttt tattacgccg ccacgctgcc agccagcgta gcatcagcgc catcgccgga
    13081 accggacgca ccggcaggcc acggcacagc cacggatgcc agaacaggac ggtcagacag
    13141 gaggcgctca acccgacggc ggcaaaaatc gccatctggc gaatgccggg gaagggggcg
    13201 agcatcataa tcagatacgc cgccacggtg gtgagcagcg ccagtagcaa ggcattgcgt
    13261 actttcgcca ggctttgcca cggcgaaacg tcgttgccgt gaaccatccg ctcggtgaga
    13321 taatagagcg tgtagtcagc ggaaatgccg ataacgctca tgctcatcac cagcgtcatc
    13381 aggtgtaatt caccgaaaat caataaagtg gcgaccgttc ccgccagcgc gccgatgccg
    13441 atggaaatca cgcaaagcag caacgggcgt aaagagcgga acaccgccac aatcagcaat
    13501 atcaccccca gcagcgtagc cacgcccagg gtggagatat cctgcttcgc ctgttggctg
    13561 gcgtaatcgc tgtaaaacac cgtgccgcgc gagagcaact gtgcctgcgg gtaacgcgtt
    13621 ttcagatcct tttccagcgt attcagggtc gtgatcagct ggtgggtttg ctgcatatca
    13681 aacgacgatc ccgccagttc gccgtgcagc agataccagt agttgccctg gggatcctgc
    13741 gtcaccagcc aaccgtccat cagccgcaaa cgctggccgt ttttcgccat tgccagctgc
    13801 gagccgcgca ttaacatcag cggatcgttt tgcagctcct tgccgcttac gccggagaat
    13861 gcggaataaa gctgggagag gatccactgt gcctgcgctt cgccgccgtt ttgcaggcgg
    13921 gcgcgggtgt tggggtcaat caggccgttg cgatgctgcc agaaaaacgc tccccacgcc
    13981 tgctggctgg cggcatccat tggcccttta acgtcgccga gcgcagcgga tttttgcagc
    14041 agcgtcagcc actcctgagc gaccaaagga ttagcctctt taccggggct gaccagccac
    14101 accagttggc ggtcaagacg ctgcataaag ccgtcattca gcgccggagg aatatcgccc
    14161 atcgtctgtt tgggtaacat agccagcacg ctactgttca gccgtgattg cggcagcagg
    14221 atcagcaacg ccaccgccat gaccaggcag actagccccc ataacagcgc ggggcgttta
    14281 ctgggcggca aaacgttggc gttcgtcatc ggtcagttgc gctggcgtca gttgatgttg
    14341 ggtaagagca atatcggtgc gatcgccctg tttatcatta agttgaatgc tctccagata
    14401 ggttttcccg gcgagatcga tggtgttgaa aattttatcc agcggcgtgg tggtcggcgt
    14461 caggcgcagc gtccagcggc cttcgccttt gtcagcaaat tcgacgcgga agttttgttc
    14521 cagcactttg cgatcggcct ggaacaacgc gcgcagcagg tggttaaact ggaacatctg
    14581 cgggttgttt tctgccgtga tgatttgcgg cggctgaccg ttgatcacct gcaccatgcg
    14641 tttatcgtcc agtaatagtt gcatggggaa cggtgaggtt tgatcccaca acagcccctg
    14701 gtcgcgggcg atcaacatct ggccctgaga tcgcagcggc tgcggcagat ctttaatcgt
    14761 ccgggtttga tcgaaatggg cgcggatcac cggttgttcg gtaaagcgtt gctgaagatc
    14821 gtccagggtc agggcgctca caaacgggct aatcagcagc gccagcagcg gtaaaaattt
    14881 catggtttga ctcccatgcg ttcaaacaga atatcggggc tgacaaagca cagttcgcga
    14941 ctttgctcgt cgaccgcgac ctgaatggtg tatccggtgg tggcgcgttt gcccgtttcg
    15001 gcatcgaaaa tctgatagcc aatccgcaga cggttttcga actcttcgat atgcgcacgc
    15061 acgcggatgc gttgctcaaa ggtgagggcg tggcgatatt tcacccgcgc atcgaccact
    15121 ggccacaaat aaccggactc tttcatttgc cgatagccgt aattgaactg gttgagcagc
    15181 gcctcgcgag ccacttcaaa gtagcgaaaa tagttaccgt gccaggccac gcccatcata
    15241 tcgacgtcgt gaaagggaat ggtcagctcg acttctgcgg taaatcgggg atcgttcagc
    15301 accctttact ccttgtcctg aatttccggc agttgccaga aatcgaaaaa attaaaccag
    15361 tcgagaggcg actggagcgc gtaatgttcc agacgcgcgg cgtaatgatc gatagcgttt
    15421 tgcagcgcct gttggcgttc gccgcgcggc agctgcagtg ggtcggcaaa gctttcgcag
    15481 tgaatatgca gcttgccgtg ctggcgcagg gcgaaaatca gattcaccgg gcagcgcaaa
    15541 atagaggcga gaataaacgg cccctgtggg aaaggcgcag gctggcccat aaacgaactc
    15601 cagcagacgc gccagtcgcc gccgcgttgc gggttgacgg cgatgcggtc accgacaatc
    15661 gccacccatt cgccgttatc cagcttctct ttcagcagga tggcggtttc tgggccgata
    15721 tctgttaccg gcatcaggtt aatgcctgcc tgaggagcca tctcctgcat tatctgttta
    15781 aagcgttggg cgttttcgct aaacaccagc gcgttaatgg ttttgtagcc ctgaattttt
    15841 gccagcgccc ggcacacttc cacatcgcca agatgcgagg ccagcagcaa tttgccgcgc
    15901 ggatcgcgga cgtcaagcgc tgcttctgcg cctggcgcaa acagcacatc acgccctggt
    15961 tgtagctcgc cgcgccagct ggcgattttg tcgagcatgg cattaccgaa acgtagaaag
    16021 tgctgatagc tgttgagttt tgccgcctgt ggttgatgct gtcgtacacg ggcgagccag
    16081 tcttgcgacg ctttgcgcgc acgagaagca gtgagccagt agacgcccac caccgggtaa
    16141 agcagcgcgc taaacgccgt tcttcccagc agacgccaga ccagcagcat caggcgcatt
    16201 ccccataatc ctttcacttc actctgccgc gcccagtggc aggaagagcg gcgcatcagc
    16261 agtgaaggaa tacgcggcaa catgccgaaa aacagacgcg tgtgcatgag cgagatgcgg
    16321 acgttatctt tcagggcatc aaaatgcgaa agcccgtcca gtggataggt gacgcgggtc
    16381 ggcacgaaat agctggtatt tccctgccag tagaggcgca ccatcacttc ggtgtcgaaa
    16441 tccatccgct tgccgatggt ggcgtgtttt gccagttgca gcgttggcgc aaccggataa
    16501 acgcgaaaac cgcacatgct gtctttcagt tgcagggaga gcgtttcgat ccatacccag
    16561 acgtgggtga cccagcgccc gtaaagccgt gagcggggga tggagtcatc gtaaattggc
    16621 tggccggaga tcagtgccgc aggttgttgt tcagcgagag ccagcagttt agggatatct
    16681 tcaatcgcgt gctgaccgtc agcatccacc tgcaccgcat ggctgaaccc tgcgtctgcc
    16741 gctgcctgta agccacgcat caccgccgcg cctttgcctg cgttttctgc caggcgaatt
    16801 aaggtcacgc caggctgttc ggcaagcaga ctgtccagtt gctgttgtgt ggtggcatcg
    16861 ctgccgtcat ccaccacaat acagggcaga ttaaatggct taagacgcgc cagcacgccc
    16921 ggcatcatcg cgccgtggtt gtagcagggt atcaacacgc agggagaaaa gtttaccgac
    16981 ataggcggat cttcccgcta ctggcggtgt ggcgggcgtc accgtcgtga cgctgatagc
    17041 taaagctcag aatctggcgt tcttcctgcc agttaagcgc gagcgtgacg cggttttccg
    17101 gcagcaacgg agactggaat tttatgttct gaatggagtg aaaacgccag ccaggcgcga
    17161 gcagattgag cgcgtagctc atcgcccagt cgatttgcgc aactcctggg agtaatggtt
    17221 gcacggcaaa atgaccgcga aaccagaaca gatccgccct gagatgcaga acaatttcaa
    17281 ggtgattcgt ttgtgcctga tggcgctcga tttcatgggg cttcatgaaa taactcctgt
    17341 aattgcgcat agacacgctt gttcatactg tttactggca tttcatcaat aacgcgccag
    17401 taacgcggaa tagcaactgg ctccagcgtc gggcgcagta aacgccgcca ggtcatttcc
    17461 tggctatgcc cgccgccgtt ctgccattgc agatgagctt cgtcatccag caccagcaaa
    17521 acgcctatgc tctgacgccc gccgcgcgta acgggaactg ccgccgcctc gcgaatgccg
    17581 tccagcgcca gtagacgttg ttctacttcc tggagtgaaa tacgtttttc ttcaatctta
    17641 acgatgcgcc cacgacgccc catcagatgg aactgaccat cttcgctaaa ctgtaagata
    17701 tcgtcgagta acatgccgtt atcttccgct atcagcggtg aaaagaggcg aaaggcatcg
    17761 ccttcggcct agaactgcat acctgggagg ggtagccagc ggcgttgctc ttgttcgcgg
    17821 tagcgccagg cgatgacgcc agtttccgtg ctgccgtaga tttcatcagg ccagacgcgc
    17881 agccagctcg cggtatgttg tacatcctgc cacggcagct caccgccagc agagagaagc
    17941 acctgaacgg gtggcggaga aagctgggta tccaggcgct tcaaaaatgc cgggctgctg
    18001 atgaagatat agcgatgctg atgactcaac gcggcaaact gttcgacata ccagagcatg
    18061 gcggcgtgca gcggtaatcc caacgccatg ggcaggaaaa cgcgaaaagt caggccgtac
    18121 aaatgctgcg gcaatacgga accgacgaca cgacaacccg ccagacgtgc gcctaatcgt
    18181 tcagccagca gttctgcttc acgatccagc aaatgaacag gtttaatcgc ccgtttcggc
    18241 tgtcccgtgg aaccggaggt aaacagctcg ataaaagcct ctggcgctat agcggcaaag
    18301 gtgaaggatt gtgtcgcgat ttgtggagaa cttgctacca acagcaatga accttgccag
    18361 ttaagttcgc tgtcgctaag cacaccatca aaaagttcac gttgctcgtt aagctggata
    18421 acacggttat gccccggaag caccggcgtt tttccggcgt gtagcgtggc gagcagggca
    18481 acgataaaca gatagctgtt ctcaaagcac agcgcccagc gttcacccgg ctgttgctgc
    18541 aagcggcaga taagttgcgc gacatcgtga cgcaaatcgc ccagcgtcca ggtactttcg
    18601 ttcagccacg caatgggtgt gtcatcaggg cgtggcgcag tcagccagcg tgcaagcggt
    18661 aaggtctgct tcatgcgcga tctcttttca tcacctgacg gcgcagcagc cattcaccag
    18721 ccattaacgt ccccatcaac aaataagcaa tcatgccgtt ccaggcggtc cataacgaca
    18781 tatcggcata aagcgcggta aagagggcaa ttccaccgtt aatgatgaaa aagccgcacc
    18841 agatttgcgt gacgtggcgg gtatagcgca cgcccttttc cgggaggtct ggctcctgca
    18901 accgcgccag ccgctcaact atcggcattg ccgaccacag cgaaccgcca aaaaccgcca
    18961 gcatgaccgc gttaaccact accgggtaaa acagcagtag ctggtgcgtt ttcaacataa
    19021 agctggcgac acataatgcg atgcccacga cagcgacgag ttgcgtgacg gcttgtaaag
    19081 gccccgcctg gcgacgggtc tggcgcagac gcaacaaaag cagcaacgcc attagcggta
    19141 acagccagtg caggccgtta tgcgctaagc caaaccaaat cacaaacggc caggcgagca
    19201 gcaataatcc agtcagcagc ttaatcatcg gtagcgagcg aatgcccgac atcagatcag
    19261 gcttcttgta gcaggcgttc tacagcctcg acgacatcct ggactgtacg cactgcttta
    19321 aactcttccg gcttgatttt cttaccggtt ttcttttgca ggtgcacaat catgtcaacg
    19381 gcgtcgatgc tgtccagttc cagatcttcg tacaggcgcg tttcaggttt gatgtcctgc
    19441 ggatcgattt caaacagctt aaccagcagg gctgagactt cctgatagac ggtttgttgt
    19501 tctgtcatga tggctttcct caggcacgtt gcgcagcaat gaaagaggcc agagtggcga
    19561 cggagaaaaa gtgctgacgc atctcttcgc tttccgcaga gagcacgatg ccgtactcat
    19621 tttttactgc cagtcccagt tccagggcat cgatagagtc cagccctaaa ccgtcgccaa
    19681 acagcggcgc gtcggtatcg atatcatcag gcgtcaactc gtccagattg agggtgctga
    19741 tgatgaggtt tttaatttcc agataaagcg cttgcattat ttattcctga gagaggtaaa
    19801 caaccttgtt gtaattgaag caaaagatgc cggtttaact gccttgctgc cagcgccggt
    19861 tcttgtgaag ttgcatcgta aaatttattg atttcaaccc gaccatggac tgcaaccgta
    19921 aaaaacggcc ggtcaggtgg aacgtcatac catttacttt gtttatccag cagatgctcg
    19981 ctacagccga ttgtcacgat gcgtaaatcg ctggcgcagc gcacggcaat attcgccgcc
    20041 ccgcgctgca gcgtcatttt ttcaccgggc ttcgttcgtg tgccttcggg gaaaatcagg
    20101 atagtgtcac cctgtgccag tcgctgttgg cagcggggta acagagtttc agcttcgctg
    20161 ttgattaaat aatctgtcgc ccgcacgacg ccgccaagaa aaggattttt taacagcgcg
    20221 cttttcacca ggcagtcagt ttctggcatg actgacgcca gcagcacgta atcgatcagc
    20281 gtgggatgat tggctacgac gaggcaaccg cgttcctgat gtaagatttc agcgccatca
    20341 atacgataat ccagtacgcc aagccctttc gcgacagtta aaaacaggcg aaaactggcg
    20401 gcaatgctgc gacgcgccaa acgacggcgg cgggaagtat ctcacaccaa aactaacaat
    20461 atgttaaacc acacgacaga aagcagcagg ccgccgagac caaacagggc aaaacataac
    20521 ccggtcatga ccagtcgcca ggtccactcc agacgtgaca tcagcttact catctgtggc
    20581 tccagcgcca ttgcacgcgc tcgccaggca gtgaaaatgt gtctacattt tgtaaatagt
    20641 gctgtaaaaa caacatgctt tgcggcagtg ttgtttcatt gcccgcaatg gctgactgcg
    20701 tttcacattg ccagtcatcg ccagcttcaa tcaccagcgc gacggcgtac ggccaggtcg
    20761 gcatttctga tggcagttgt gggtgataaa actcgggcag gaaaccgtca aaatcgacca
    20821 tcagcacgcg ctggtatccc gcttgtagca ggctaagcac ttcacacaaa ccttgctgga
    20881 aactatcgcg accagccgaa agcgatgacg aaacgctcgg ctgtttggcc gcaatggtca
    20941 ggttacccac ggaagagttg tgtacggaga gcgcgaagtc cgtcggagaa agcgcctgtt
    21001 cggttgccag tgcatgaaca atgcggtaat tgcgctccag ttcgccatga cgactggtat
    21061 acaaaacggc gtctggttga tggcggcgta gcattgccag accgcattca acagccagtt
    21121 tgctcccgga gctgaggcgg cgggcggtca tcattggcag ttcacttaat tttgccaacg
    21181 gtgctgtcgg gtcaattgcc tgaggctgac gtgaccactc ctgccattgc agcgtttcac
    21241 taagtccagg cgctcttgcc tgccagtcga gaatgtttaa tgcaaatttc atcaacgcgc
    21301 gcccgcggta aaccaaaatt attaagtgac gattatttct tttggcatat caaaagtgta
    21361 tgcccaacgc ctaaattatc gtggcgctct gctacctgga atcctgcttt attcagatag
    21421 tcgtaaaatt tctctgcact atagaaacgg ctgttaccgt ttgccatgca ggtaaaataa
    21481 agtgaggacg cgttcaggct aaacgatgct gcttcaaacc gctgcgcatc ccagaacaac
    21541 tccatgatgc aaacttttgc gccaggcttc attacgctgg caattttgct gagtatgcta
    21601 attatttgtt cgggtgaaaa acagtctaaa aattggctca tccaccagat atcagcctcg
    21661 cccggtaatg gggcatcgct tagcatatcc accgcatgga aatctatgcg atcagaaaaa
    21721 cctgcattcg cgatattttc tttcgctagt acgatttgtt gtggcagatc taataacgta
    21781 acggcgatgt tttcattgta tttgcagcaa cgcaaagccc acttacccgt attgccgcct
    21841 acatcgtaca atgttgtggg gttgttggcg aatacataag gcagtgcggc gttaaatgcg
    21901 ccatctgaat agtaatgatc aaacgcaaac cagctttcac gagcggcgtc tggtaattgc
    21961 gaaagtgccg gatatatcgt cgaccagtcg ccaaatactt ttaacccgga aggttttcct
    22021 tcattgagtg aatcggcgag gaagaagagc ccctggtaac agacatcctg ggtgaaatcc
    22081 atattcacgc gagtcatagt gtcatgcagg aggtaatgac cgattttcgc caggtaataa
    22141 attccctctt tacaggtaat aattcgcccg cttaagccca tatctaacag aacgctggta
    22201 gcatattcgt tgatatggct atgttcggtg atatcagcca gcgttgcgcc atgtttgcct
    22261 tgccgatcca gatatgaaag tacacccgca ttacgtaggc agagtgctgt ttgaaaaagc
    22321 ataggggcaa aggcaatgcg ttgagcttcg gtaatcgcat ccagtgcgct aagagaatcc
    22381 tgttcgtaca tgttgggcac aacctgttaa gagggaatta aattattatc ggaccgcaac
    22441 ggcccgactt taggaagaat tataaatctg cgcccgctga caaattcagt tgaatatctt
    22501 tgtcgagatt gcggacccag cgattgtagt ttttatgaat ttgtccgcct gacgcttgta
    22561 ggttctggct attttcataa ttgatatcgt agctggatgc cgtgtagtta atgcgtattt
    22621 ccgctgaatg atcgcgtgac tgttgacgtg ctttaattat gccttgtccg gcttcggaca
    22681 taatccattt gcgttcaacg ccagctttca gaatcgcttt tttcacttgt tcctgggtat
    22741 gaccagcgct gacggttgag tgaacctgat caattggcgc agtacgcgcg caacctgcta
    22801 aagcacccac aacagcaata gccgcaaacc atttcatgta atgtctcatt agaaccccat
    22861 taaagtattg tttttacgag taaagc
//
LOCUS       DBFIFI010000061        21918 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.061, whole genome shotgun sequence.
ACCESSION   DBFIFI010000061 DBFIFI010000000
VERSION     DBFIFI010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21918
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.061"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            17..718
                     /gene="elfD"
                     /locus_tag="N7B39_002222"
     CDS             17..718
                     /gene="elfD"
                     /locus_tag="N7B39_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HGU2422201.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSTWAAGKGGVGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKLVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            743..3342
                     /gene="elfC"
                     /locus_tag="N7B39_002223"
                     /pseudo
     CDS             743..3342
                     /gene="elfC"
                     /locus_tag="N7B39_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            3333..4403
                     /gene="elfG"
                     /locus_tag="N7B39_002224"
                     /pseudo
     CDS             3333..4403
                     /gene="elfG"
                     /locus_tag="N7B39_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            4431..4958
                     /gene="ycbU"
                     /locus_tag="N7B39_002225"
     CDS             4431..4958
                     /gene="ycbU"
                     /locus_tag="N7B39_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2422202.1"
                     /translation="MFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDVATKSA
                     LQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAPTLLKL
                     TDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKSTKAGAT
                     GGNATAVLYFDLVYQ"
     gene            4966..5481
                     /gene="ycbV"
                     /locus_tag="N7B39_002226"
     CDS             4966..5481
                     /gene="ycbV"
                     /locus_tag="N7B39_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2422203.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFSHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            5447..6183
                     /gene="ycbF"
                     /locus_tag="N7B39_002227"
                     /pseudo
     CDS             5447..6183
                     /gene="ycbF"
                     /locus_tag="N7B39_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
     gene            6294..7304
                     /gene="pyrD"
                     /locus_tag="N7B39_002228"
     CDS             6294..7304
                     /gene="pyrD"
                     /locus_tag="N7B39_002228"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HGU2422204.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            7478..8020
                     /gene="zapC"
                     /locus_tag="N7B39_002229"
     CDS             7478..8020
                     /gene="zapC"
                     /locus_tag="N7B39_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HGU2422205.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTNEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(8017..9126)
                     /gene="ycbX"
                     /locus_tag="N7B39_002230"
     CDS             complement(8017..9126)
                     /gene="ycbX"
                     /locus_tag="N7B39_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HGU2422206.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDSSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PEAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILTTAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            9370..11478
                     /gene="rlmKL"
                     /locus_tag="N7B39_002231"
     CDS             9370..11478
                     /gene="rlmKL"
                     /locus_tag="N7B39_002231"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HGU2422207.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKEPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            11490..13397
                     /gene="uup"
                     /locus_tag="N7B39_002232"
     CDS             11490..13397
                     /gene="uup"
                     /locus_tag="N7B39_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU2422208.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKNEEPAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            13527..14780
                     /gene="pqiA"
                     /locus_tag="N7B39_002233"
     CDS             13527..14780
                     /gene="pqiA"
                     /locus_tag="N7B39_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HGU2422209.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCGTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14785..16425
                     /gene="pqiB"
                     /locus_tag="N7B39_002234"
     CDS             14785..16425
                     /gene="pqiB"
                     /locus_tag="N7B39_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HGU2422210.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     VLDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            16422..16985
                     /gene="pqiC"
                     /locus_tag="N7B39_002235"
     CDS             16422..16985
                     /gene="pqiC"
                     /locus_tag="N7B39_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HGU2422211.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVAVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            17241..17408
                     /gene="rmf"
                     /locus_tag="N7B39_002236"
     CDS             17241..17408
                     /gene="rmf"
                     /locus_tag="N7B39_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HGU2422212.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(17478..17996)
                     /gene="fabA"
                     /locus_tag="N7B39_002237"
     CDS             complement(17478..17996)
                     /gene="fabA"
                     /locus_tag="N7B39_002237"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HGU2422213.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(18065..19825)
                     /gene="ycbZ"
                     /locus_tag="N7B39_002238"
     CDS             complement(18065..19825)
                     /gene="ycbZ"
                     /locus_tag="N7B39_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGU2422214.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            20011..20463
                     /gene="matP"
                     /locus_tag="N7B39_002239"
     CDS             20011..20463
                     /gene="matP"
                     /locus_tag="N7B39_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HGU2422215.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(20538..21578)
                     /gene="ompA"
                     /locus_tag="N7B39_002240"
     CDS             complement(20538..21578)
                     /gene="ompA"
                     /locus_tag="N7B39_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HGU2422216.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
BASE COUNT         5597 a         5180 c         5772 g         5369 t
ORIGIN      
        1 ttaagagtga ctatttatga aaacttgcat aacaaaggga attgtgaccg taagtttaac
       61 ggcaatatta ctcagctgtt cgtccacatg ggcggcaggt aaaggcgggg ttggacttgc
      121 agcaacacgt cttgtttatt cggaaggtga agagcaaatt tcactgggtg tacgtaacac
      181 cagtccggat gttccttatc tgattcagtc atgggtgatg accccagata ataaaaaatc
      241 agcggacttt attattacac caccgctatt tgtgctgaat ccggcaaacg agaatctgtt
      301 acgcattatg tacattggcg cgccgttggc gaaagacaga gaaactcttt tcttcactag
      361 cgtacgggca gtcccttcaa caacgaagcg ggaagaaggc aataccctga agattgccac
      421 acaaagcgtc atcaaacttt tctggcgacc aaaaggttta gcgtatccct taggcgaggc
      481 tccggcgaaa ctgcgttgca cttcgtcagc tgacatggtt acggtcagta acccaacacc
      541 ttatttcatt accctgacag acctgaaaat aggtggaaag ttagttaaaa atcaaatgat
      601 ttcccccttt gataaatacc aattctctct gccaaagggt gccaaaaata gcagcgtaac
      661 gtatcgaacc atcaatgact acggggcgga aacgccgcaa ctcaactgta aatcgtaatt
      721 cgtcttcagt taagagagcg agatgtatag aactcaccga caacacagcc tgttaagctc
      781 tggtggagtg ccatcgttta ttggtgggct ggtggtgttt gtgtcggcag cgttcaatgc
      841 acaagctgaa acctggttcg atccagcctt tttcaaagat gatccctcaa tggtggccga
      901 tttgtctcgt ttcgaaaaag gacaaaaaat aacgccaggg gtttatcgtg tcgatattgt
      961 tctgaatcag acaattgtag atacgcgcaa cgtcaatttt gttgagataa cgccagagaa
     1021 ggggattgcc gcctgtttga cgactgaaag cctggatgca atgggcgtga atactgatgc
     1081 atttccggct tttaaacaac tggacaaaca agcgtgtgcg ccattggcgg agattattcc
     1141 ggatgccagc gtaactttta atgtgaataa actccgtctg gaaatttcag taccgcaaat
     1201 tgccatcaaa agtaatgctc gtggttatgt ccccctgaac gttgggatga agggatcaac
     1261 gcgctattac tgggatattc atttagcggg gctaacagta ttcatagcag cgcagacagt
     1321 gattctggcg acagttattt tctgaattta aacagtggcg ttaatttagg cccatggaga
     1381 ttgcgcaaca attcaacatg gagtcgtagt agtggccaaa ccgcagaatg gaagaatctc
     1441 agcagctatt tgcaacgggc ggttattcct ctgaaaggcg aactgaccgt aggtgatgat
     1501 tatactgcag gcgatttttt cgatagtgtc agctttcgtg gtgtgcagct ggcgtcagat
     1561 gacaacatgc tgccagacag cctgaaaggg tttgcgcctg tggtgcgtgg tatcgccaaa
     1621 agcaatgccc agataacgat taagcaaaat ggttacacca tttaccaaac ttatgtatcg
     1681 cctggtgctt ttgaaattag tgatatctat tccacgtcgt cgagcggtga tttgttagtt
     1741 gaaatcaaag aagcggacgg cagcgtcaat agctacagcg tacccttttc cagcgtgcca
     1801 ttactccagc gtcaggggcg aatcaaatac gcggtgacac tggcgaaata cagaaccaat
     1861 agtaatgaac agcaggagag caaatttgcc caggccacgt tgcagtgggg cggaccgtgg
     1921 ggaacgacct ggtatggtgg tggacaatat gctgaatatt accgtgccgc catgtttggt
     1981 ctgggattta acctcggcga tttcggagca atttcgttcg atgtgaccca ggcgaagagt
     2041 acgctggcag accaaagcga acataaaggt cagtcatatc gttttctgta tgccaaaacg
     2101 ctcaaccaat tgggtactaa ctttcaattg atgggctatc gctattcgac gtcgggtttc
     2161 tacacccttt ccgacaccat gtataaacat atggatggct acgaatttaa tgacggtgat
     2221 gatgaagata cgccgatgtg gtcgcgttat tacaatttgt tttacaccaa acgtggcaaa
     2281 ctgcaggtca atatctccca gcaattaagc gagtacggtt cgttttattt aagtggtagc
     2341 cagcaaactt actggcatac cgatcaacag gatcggctat tacagtttgg ctataacacg
     2401 caaattaaag atctctcgct ggggatctcc tggaactaca gtaagtcccg tggccaacct
     2461 gatgctgatc aggtgtttgc acttaatttt tccctgccac tcaatctgtt gctctccaga
     2521 agtaatgata gctataccag caaaaaaaat tacgcctgga tgacctctaa caccagtatc
     2581 gataacgaag ggcacactac acaaaacctg ggtttaacgg agacactact cgatgacggt
     2641 aacctgagct acagcgtgca acagggatat aacagcgagg ggaaaacggc taatggcagc
     2701 gccagcatgg actacaaagg ggtgtttgca gatgcccgag tgggctacaa ctacagcgat
     2761 aacggcagtc aacaacaact gaactacgct ctttcaggca gtttagttgc ccattcacag
     2821 ggcattaccc tggggcaatc actgggggaa actaacgttc tgattgcagc accaggcgca
     2881 gagaatactc gtgtggcgaa cagcaccggg ctgaaaactg actggcgcgg atataccgtt
     2941 gttccttatg ccacttctta tcgtgaaaat cgaatcgcac ttgatgcggc gtcgttaaaa
     3001 cgtaacgtgg atcttgaaaa tgcagtagtc aacgtggttc ccaccaaagg ggcgttggtt
     3061 ctggcggagt tcaatgccca tgcgggagca agggtattaa tgaaaacatc aaagcagggt
     3121 atatcgctgc gttttggcgc gatagcgacg ctggacggcg tacagactaa tagcggcata
     3181 attgatgatg atggctcgct ctatatggcg ggtttaccgg cgaagggaac aataaccgtg
     3241 cgctggggcg aagctcccga tcaaatttgt catatcagtt acgagcttac cgaacaacaa
     3301 attaactctg cgattacgcg aatggatgcc atatgcagat aatctttgga gaaaaatgcg
     3361 tgtcattact acgactattt tttgccgccg tcttaatgct atggtgcgct caaaccgctg
     3421 cttatagcgg gcagtgtcat accactcagg ggaatccgta tattggcgtc aattttggcg
     3481 ttaaaaccct ggaggaagaa gaaaatacga ctggggtagt aaaagacaaa ttttatcagt
     3541 ggaacgaatc gaatgattat tatgtttcct gtgattgcga taaagacaat gtcagaagtg
     3601 gccgatgggc attcgccgcg gattcaccgt tagtctattt aggcgacaac tggtacaaaa
     3661 ttaatgacta tcttgccgcc aaagttttat tgcaggttaa aggcagttct cctacagcgg
     3721 ttcctttcga aaacgtgggg actggggcag atacccggtg gcatatttgt gaccccggcg
     3781 gtcaacgttt aggcggccag ggagctagcg gtaatagcgg tagcttttcc ctgaaaatat
     3841 tgcagccgtt cgttggttcg gtcgtcattc ctcctatggc gctggcgcga ttatttgaat
     3901 gctacaacat acccgcaggt gattcctgca cgactacagg cacaccggtt ttagtgtatt
     3961 acctgtctgg tactatcaat tcacttggct catgttccgt caatgccgga gaaacaatcg
     4021 aggtcgatct gggcgacgta tttgcggcta actttcgtgt tgtagggcat aagcctcttg
     4081 gggccagaac ggcagaactt gcaattccag tcaggtgtaa cacgggaaac gcggggttag
     4141 ttaacgtcaa cctgagtctg acggcaacca cagaccccag ctatccccag gcgattaaga
     4201 cgtcacgtcc tggcgtgggc gtggtggtga ccgatagcca gaacaacatt atttcccctg
     4261 ctggtggaac attaccgctc tctattcctg atgatgcaga cagtatcgcg tgaatgaatg
     4321 tctatccagt cagcacgaca ggtgtaccac cagaaaccgg gcgatttgaa gccacggcaa
     4381 cggtgagaat aaattttgat taacgcgtga acgtatgaag aaaaaaaacg atatttcagt
     4441 gcgttatctt gttctttagc attcttaaca tccatgtcgg gatggctggg cctgaacaag
     4501 ttagtatgca tatttatggg aatgtggtcg atcagggctg tgatgtcgcc accaaaagtg
     4561 cattacaaaa tattcatatt ggtgatttta atatcagtga ttttcaggcc gcgaataccg
     4621 taagcactgc tgctgatttg aatatcgata tcaccggttg tgccgctggt attactggcg
     4681 cggacgtcct ttttagcggc gaggctgaca cccttgcgcc gacactgctc aaactaactg
     4741 acacaggcgg aagcggtggt atggcaacgg ggattgccgt gcaaattctt gatgcgcaaa
     4801 gtcagcaaga aatcccgctc aatcaggtcc agcctcttac gcccttaaaa gccggggata
     4861 acacactcaa atatcaactt cgttataagt ccacaaaggc gggagcaacg ggcggtaatg
     4921 cgacggcggt tctctatttt gatctggttt accagtgaag gagtgatgtt gaaacggata
     4981 atctggattc tgttcttact gggattaacg ggggggtgtg agctatttgc ccatgatggc
     5041 acggtcaaca ttagcggatc gtttcgccgt aatacatgcg tgctggcaca ggatagcaag
     5101 caaatcaatg tgcagttagg cgatgtctca ctgacacgtt tttctcatgg gaattatggc
     5161 ccggagaaat ctttcatcat taatttgcag gattgcggaa cagatgtcag cacggttgac
     5221 gtcacctttt caggaacccc cgacggagtg cagagcgaaa tgttgtccat tgaaagtggt
     5281 acggatgcag ccagcgggct ggcaattgcg attctggatg atgcgaagat attgattccg
     5341 cttaaccagg cgagtaaaga ctacagccta catagcggta aggtgccatt aactttttat
     5401 gcgcaattgc gacctgtcaa tagcgatgtg cagtcgggta aagtgaatgc cagcgcgaca
     5461 tttgtacttc attatgacta atacgtggaa cagattggcg ctcttgattt tcgccgtttt
     5521 atcgttgctg gtggcgggtg aattgcaggc aggagtggtg gtcggcggaa cgcgatttat
     5581 ctttccggca gacagagaat cgatatctat tttactgact aataccagtc aggaatcctg
     5641 gcttattaac agtaaaatca accgcccaac gcgttgggcc gggggtgaag cgtcgacagt
     5701 gccagcacca ttactggccg ctccgccact tattctcctg aagcccggta cgacaggcac
     5761 gttgcgcttg ctgagaacgg aaagcgacat cttgcctgtg gatcgcgaaa cgctatttga
     5821 gttaagcatt gccagcgtgc catccggcaa agttgaaaat cagagcgtaa aagtggcgat
     5881 gcgctcggta tttaaactgt tctggcgacc cgaaggttgc cgggcgaccc gctggaagct
     5941 tatcaacaat tacgctggac acggaagtcg cagggtgtac aactcactaa cccaacgcct
     6001 tattacatta acctgattca ggtgagcgta aatggtaaag cgttaagtaa tgtgggagtt
     6061 gtgccgccta aaagccagcg tcagacaagc tggtgtcagg caatagcacc gtgtcatgtt
     6121 gcctggcggg cgataaatga ttacggcggg ttgagtgcaa agaaggagca aaatctgccc
     6181 tgaaacaggt tcggaaaacg tttgcgtttt ttttgccgca ggtcaattcc cttttggtcc
     6241 gaactcgcac ataatacgcc cccggtttgc acaccgggaa tccaggagag ttcatgtact
     6301 accccttcgt tcgtaaagcc cttttccagc tcgatccaga gcgcgctcat gagtttactt
     6361 ttcagcaatt acgccgcatt accggaacac cgtttgaagc actggtgcgg cagaaagtgc
     6421 ctgcaaaacc tgttaactgc atgggcctga cgtttaaaaa tccgcttggt ctggcagccg
     6481 gtcttgacaa agacggggag tgcattgatg cgttaggcgc gatgggattt ggatcgatag
     6541 agatcggtac cgtcacgcca cgtccacagc caggtaatga caagccgcgt ctctttcgtc
     6601 tggtagatgc cgaaggtttg atcaaccgta tgggctttaa taatcttggc gttgataacc
     6661 tcgtagagaa cgtaaaaaaa gcccattatg acggcgtcct gggtattaac atcggcaaaa
     6721 ataaagatac gccagtagag cagggcaaag atgactatct gatttgtatg gaaaaaatct
     6781 atgcctatgc gggatatatc gccatcaata tttcatcgcc aaatacccca ggattacgca
     6841 cactgcaata tggtgaagcg ctggatgatc tcttaactgc gattaaaaat aaacaaaatg
     6901 atttgcaagc gatgcaccat aaatatgtgc cgatcgcagt gaagatcgcg ccggatcttt
     6961 ctgaagaaga attgatccag gttgccgata gtttagttcg ccataatatt gatggcgtta
     7021 ttgcaaccaa taccacactc gatcgttctc tggttcaggg aatgaaaaat tgcgatcaaa
     7081 ccggtggctt aagtggtcgt ccgcttcagt taaaaagcac cgaaattatt cgccgcttgt
     7141 cactggaatt aaacggtcgc ttaccgatca tcggtgttgg cggcatcgac tcggttatcg
     7201 ctgcgcgtga aaagattgct gcgggtgcct cactggtgca aatttattct ggttttattt
     7261 ttaaaggtcc gccgctgatt aaagaaatcg ttacccatat ctaattattt cttcgaattc
     7321 ctctttataa gataatcagg gctttatttt cagccctggt tgttttatat tcattgctgt
     7381 tgcttattta gacattttgt acttttattg ttgaggttat taagcgaagc gacaatggat
     7441 tgtgttgctg cggttttata gggtagggga gaggcagatg cgaattaaac cagacgataa
     7501 ctggcgttgg tattacgatg aagagcacga tcgtatgatg ctcgatttag ccaatggtat
     7561 gctatttcgc tcacgttttg cgcgcaaaat gttgactcca gatgcctttt ctcccgcagg
     7621 cttttgcgtt gacgacgccg cgctctattt ctcttttgaa gaaaagtgcc gcgattttaa
     7681 tttatctaaa gaacaaaaag ccgaactggt gctgaatgca ctggtagcaa ttcgctatct
     7741 caaaccgcaa atgccgaaaa gctggcattt tgtttcccat ggtgaaatgt gggtgcccat
     7801 gccgggcgat gcggcctgcg tttggttgag cgacactaac gagcaagtga atttactggt
     7861 cgttgaatct ggcgagaatg ccgcgctatg cctgctggca caaccctgcg ttgttattgc
     7921 gggtcgcgcg atgcagttgg gcgacgccat taaaatcatg aacgacaggc tgaaaccgca
     7981 ggttaatgtt gacagcttca gcctcgaaca ggcagtctaa cgcgccaact taagtgcagt
     8041 cttcggtaca cagctacagc aaagaatggt gccatcatcg cccattgctg attttttcag
     8101 cggcgtgact tcgccttcta aaagctgaac acggcaactt ccacaaatgc ccgcgcggca
     8161 agaataaggg atacgaattc cctgattttc taattgctcc agcaacacct gttggttatt
     8221 tccacgaaat gcctgtccct gccagtcaat atctacattg gcgtccggtt gttgcgtgat
     8281 gttggcagta tcatcagcgg cagctgcgcc gtaaattttt gccggagccg ttgtcagaat
     8341 ttccacctca tcgccaacgc gaatcacgcc gctattacgg gcaattaaat tctgaccaaa
     8401 atcgacatcg ccgttatcct gggcagtgcg gaaagattgc aatgttttta atggttcgcc
     8461 tgccggatgt ttttgccctt tttctgggct gacggtggtg aaaatacagc ggctacaagg
     8521 tttaaccaca tcaaacacca catcaccaat gcgaatcact ttccaactat cttcttccca
     8581 ggctgacgcg ccggaaacca ccagattggg gcggaattgc tccattttta cactggccgg
     8641 acaacgttgt tggagatcac gtaacgaggc ttcgttagca agaaggtaag gatagccatc
     8701 agcaaatgac aggggtacag tgttgtggcg tttcacgcgc cgggtcattt gtggccccac
     8761 ccagcgtaat tgcacttcgc gggagaaaaa tccacttaac catttgttga tcgcttctgg
     8821 cgcaattcgc gcggtaaaat gtgtgcccca aacttcggtt ggtgcgtctt gtgtggcgaa
     8881 atcagcaaaa cgaacatatg cgctactgct atctggtgcg gtgagatgca agccatcatg
     8941 cacgggtgaa ggagtaaatc gtaccatctg gggaaactgg cgagcggtga taaacgtacc
     9001 gtcaggttcc gtgatcataa agatgcgatc gaaggccaga ccactgacat ctgccagagc
     9061 atgtgtaaga ccaatgccgc gcatcgattt aacaggatga ataaaaagcc gggttaatgt
     9121 cgccacggtg cggtcctcaa atgaaaataa gccctcaact ttatgacatg acgcgcttat
     9181 tagctataat gcgcaacaat tttcttagcg cctgtcccat taggctattt tatttgtcag
     9241 tttggccctg ggttgtgctc gaaatcctca cgtacttgtg tacgctccgg tttctccgcg
     9301 ctgtccatgt ccagactgac tgcaacaatt acgcctactg cggtaggctc ttagagtaaa
     9361 agtgacgata tgaattctct gtttgccagt acggcccgtg ggctggaaga gctgttaaaa
     9421 actgaactgg aaaacctggg ggccgttgaa tgccaggtgg ttcagggtgg ggtccatttc
     9481 aagggcgaca cacggcttgt ttaccagagc ctgatgtgga gccgcctggc ctcgcgtatt
     9541 atgttgccgc tgggcgagtg taaggtttac agcgatttag acctctatct cggtgttcag
     9601 gcgatcaact ggacagagat gtttaatcct ggcgcgacct tcgctgtcca cttcagtggt
     9661 ttgaatgaca ctatccgcaa cagtcagtac ggcgcgatga aagtgaaaga cgcgatcgtc
     9721 gatgctttca cgcggaaaaa tctgccgcgt ccaaatgttg atcgcgatgc gccggatatc
     9781 cgcgttaacg tctggctgca taaagaaacc gccagtatcg cccttgatct cagtggtgat
     9841 ggtttacatc tgcgtggcta tcgcgatcgt gctggtattg cgccgatcaa agaaaccctg
     9901 gcagccgcta ttgtgatgcg atccggctgg cagccaggaa caccgttgct cgatccgatg
     9961 tgtggttccg gtacgttgct gattgaagcg gcgatgctgg cgaccgatcg cgcaccaggc
    10021 ttgcaccgtg ggcgttgggg ctttagcggc tgggcgcagc atgatgaagc tatctggcag
    10081 gaagtgaaag cggaagcgca aactcgcgcc cgtaaaggcc tggctgagta tagctctcat
    10141 ttttacggtt cggacagcga cgctcgggtg attcaacgtg cacgcactaa cgcccgtctt
    10201 gcggggattg gtgaactgat cacctttgag gtgaaagatg tcgcgcaact gaccaatccg
    10261 ctgccgaaag agccgtacgg cacagtgtta agcaacccgc catacgggga acgtctggac
    10321 agcgaaccgg cgctgattgc gctgcatagc ctgctgggtc ggatcatgaa aaaccagttt
    10381 ggtggctgga atctctcttt gtttagtgcc tcgccggatc tgctaagctg cttgcagctg
    10441 cgtgcagaca aacagtacaa ggcgaaaaac ggcccgctgg actgcgtaca gaaaaattac
    10501 cacgttgccg aaagcactcc agacagcaaa ccggcgatgg tagcggaaga ctacgccaac
    10561 cgcctgcgta agaacctcaa aaaattcgag aagtgggctc gtcaggaagg gattgaatgt
    10621 taccgcctgt atgacgccga tctgccggaa tataacgttg ccgttgaccg ttatgccgac
    10681 tgggtggtgg tgcaggagta tgcgccgcca aaaactattg atgcgcacaa agcgcgtcag
    10741 cgtctgttcg atattatcgc tgcaaccatt tcggtactgg ggattgcgcc aaacaaactg
    10801 gtgctgaaaa cccgtgaacg tcagaagggc aaaaatcagt accagaaact gggcgagaag
    10861 ggcgagtttc tggaagttac cgaatataac gctcacttgt gggtgaacct gacggattat
    10921 ctcgatactg gtctgttcct cgatcaccgt atcgcccgtc gtatgctcgg ccagatgagc
    10981 aaaggcaaag atttcctcaa cctgttctct tataccggca gcgccaccgt gcatgcggga
    11041 ttaggcggtg cacgcagcac caccaccgtg gatatgtcgc gtacttatct ggagtgggca
    11101 gaacgcaacc tgcgtctgaa tggcctaacc gggcgtgcgc atcgcctgat tcaggccgat
    11161 tgcctggcgt ggctgcgtga ggcaaatgaa cagttcgatc tgatctttat cgatccgcca
    11221 accttctcta actcaaaacg aatggaagat gcgtttgatg ttcagcgcga tcatctggcg
    11281 ctgatgaaag atttgaaacg tctgctgcgt gcaggtggga cgatcatgtt ctcgaacaac
    11341 aaacgtggct tccgtatgga tctcgacggc ctggcaaaac tgggactgaa agcacaagaa
    11401 attacgcaaa aaacgctctc ccaggatttc gcccgtaacc gccagatcca caactgctgg
    11461 ctgattaccg cagcctgaaa ggaatagtaa tgtcattaat cagtatgcat ggcgcatggc
    11521 tgtcgttcag cgacgcgccg cttctcgata acgcagaact gcatatcgaa gataacgaac
    11581 gtgtttgtct ggtgggccgc aacggcgcag gcaaatcgac gttaatgaaa atcctcaacc
    11641 gtgaacaagg gctggatgac ggtcgcatta tttacgagca ggatttgatt gtagcgcgtc
    11701 tgcaacagga tccgccgcgt aacgttgagg gtagtgttta tgatttcgtt gccgaaggca
    11761 ttgaagaaca agcggaatac ctgaaacgct atcacgatat ttcgcgcctg gtgatgaacg
    11821 acccgagcga gaaaaatctc aacgaactgg cgaaggttca ggaacagctg gatcaccaca
    11881 acctgtggca gctggaaaac cgcatcaacg aagtgctggc gcaactgggg ttagatccta
    11941 acgttgcgct gtcgtcgctt tccggcggct ggttgcgtaa agcggcatta ggacgcgcgc
    12001 tggtgagcaa tccgcgcgta ttgctgctgg acgaaccgac taaccacctg gatatcgaaa
    12061 ccatcgactg gctggaaggg ttcctcaaaa ccttcaatgg gacgattatt ttcatctccc
    12121 acgaccgttc gtttatccgc aatatggcga cgcgcattgt tgatctcgat cgcggcaagc
    12181 tggtgaccta tccgggcaat tacgatcagt acctgctgga aaaagaagaa gccctgcgcg
    12241 tggaagaact acaaaatgcc gagtttgatc gcaaactggc gcaggaagaa gtgtggattc
    12301 gccaggggat caaagcacgc cgtacccgta atgaaggccg cgtacgcgcc ctgaaagcga
    12361 tgcgtcgcga gcgtggcgaa cgtcgcgaag tgatgggtac cgcaaagatg caggtggaag
    12421 aggccagccg ctccggtaag atcgttttcg aaatggaaga cgtttgctac caggttgacg
    12481 gaaagcaact ggtgaaagat ttttctgccc aggttctacg tggcgacaaa attgccctga
    12541 ttgggccgaa tggctgcggc aaaaccacgc tgctgaaact gatgctcggt cagcttcaag
    12601 cggacagcgg gcgtattcac gttggcacca aactggaagt ggcttatttc gatcagcacc
    12661 gcgcggaact ggatcccgat aaaacggtga tggataacct tgccgaaggt aagcaagagg
    12721 tgatggttaa cggcaagcca cgccacgttt tgggctattt gcaggacttc ctgttccatc
    12781 cgaaacgggc gatgacgcca gtacgtgcgc tttctggcgg tgagcggaac cgcttgctgc
    12841 tggcgcgttt gttcctcaaa ccaagcaact tattgattct tgacgaaccg accaacgatc
    12901 ttgatgtcga aacgctggaa ctgctggaag aactgatcga cagctaccag ggcacggtat
    12961 tgctggtaag ccacgatcgt cagtttgtcg ataacaccgt tacagaatgc tggatcttcg
    13021 aaggcggcgg taaaattggt cgttatgtcg gcggttatca tgatgcccgt ggtcagcaag
    13081 agcagtatgt ggcgctcaaa cagcctgcgg tgaaaaaaaa cgaagaaccc gccgcgccaa
    13141 aagcagaaac tgtaaaacgc agcagtagca aactaagcta taaattgcag cgcgaactgg
    13201 agcagctacc gcaattgctc gaagatctgg aggcgaagct ggaagcccta cagacgcagg
    13261 tggcggatgc ttccttcttc agtcagccgc atgagcagac acaaaaagtg ctcgccgata
    13321 tggctgctgc agagcaggag ctggagcaag cctttgaacg ctgggagtat cttgaagcgt
    13381 taaaaaatgg tggctgatcg caaaatagtc gatttaaaca gagacagctg acaacggtaa
    13441 aattgcctga tgaactgggt ttatcaggca tgaaaacaaa cactttgtta gtcatctgta
    13501 aaatagcgca tcattaagga gtaccaatgt gcgaacatca tcatgccgcg aagcacatcc
    13561 tgtgctcgca gtgtgacatg ctggtggcgt taccgcgcct tgagcatggt cagaaagcgg
    13621 catgtccccg gtgtggcaca acgttaaccg tggcgtggga tgccccccgg cagcgtccga
    13681 ccgcctatgc gttggctgca ctgttcatgc tgttgctgtc caacttgttt ccttttgtga
    13741 atatgaacgt tgcaggagtt accagtgaaa ttacattact ggaaattccc ggcgtgcttt
    13801 tttctgagga ctacgccagc ctcggcacct ttttcctgtt gtttgtgcaa ctggttcccg
    13861 cgttttgtct gataaccatt ctgttactgg tgaatcgcgc ggaattaccg gtccgtttaa
    13921 aagagcaact ggcacgggtg ctttttcaac tcaaaacctg gggaatggcg gagattttcc
    13981 tcgcgggtgt gctggtcagt ttcgttaaac tgatggctta cggcagcatt ggcgtaggca
    14041 gcagctttct cccctggtgt ttattttgtg tcctgcaact gcgcgctttt cagtgcgttg
    14101 atcgtcgctg gttatgggac gatatcgccc cgatgccaga actgcgccag ccgctaaaac
    14161 caggcgtcac ggggatacgt caggggctgc gttcgtgctc ctgttgtacg gcaatccttc
    14221 ctgctgatga acccgtgtgc ccgcgctgtg gtactaaagg gtacgttcga cgtagaaaca
    14281 gcctgcaatg gacactcgcg ctgcttgtta cgtccatcat gctgtatctt ccggctaata
    14341 ttttgcccat catggtgacg gatttattag gctcgaagat gccatcgacg attctcgctg
    14401 gggtcatcct gttatggagc gaaggctctt atcccgtcgc tgcggtgatc tttctggcca
    14461 gtattatggt gccaacgtta aagatgatcg ccatcgcgtg gctgtgttgg gatgccaaag
    14521 ggcatggcaa gcgcgacagt gaaagaatgc atttgattta tgaagttgtt gagtttgtag
    14581 gccgctggtc gatgattgac gttttcgtta tcgcggtgct ctcggcgctg gtgcgtatgg
    14641 gaggtttaat gagtatttat ccggcaatgg gtgcattaat gtttgcttta gtcgtcataa
    14701 tgacaatgtt ttctgctatg acgtttgacc cgcgtttgtc gtgggatcgt caacctgaat
    14761 cagagcatga ggagtcctga cagtatggaa tctaataatg gggaagccaa aatccagaaa
    14821 gtgaagaact ggtctcccgt gtggatattt cctatcgtca cggcgctcat tggggcctgg
    14881 gttctttttt atcattacag ccatcaggga ccggaagtga ccctgatcac cgcgaatgcg
    14941 gaaggaattg aaggtggcaa aaccaccatt aaaagccgta gcgttgacgt cggcgtggtt
    15001 gaaagcgcca cactggctga tgatttgaca cacgttgaaa tcaaagcgcg gctgaattcc
    15061 ggtatggaaa aattgctgca taaagatacc gtcttttggg tggtgaaacc gcagattggt
    15121 cgagaaggga ttagcggtct gggaaccctg ctgtctgggg tttatatcga actgcagcca
    15181 ggcgcgaaag gcagcaaaat ggataaatac gatttgctgg actcgccacc gttggccccg
    15241 cctgatgcga aaggtatccg tgtggttctc gatagcaaaa aagccgggca gctctcgcca
    15301 ggagatccgg tgctgttccg tggctatcgg gtaggttcgg ttgaaaccag caccttcgat
    15361 acgcaaaaac gcaatatcag ttatcaactg ttcatcaatg caccttatga ccgactggtg
    15421 accagcaatg ttcgcttctg gaaagatagt ggcattgcgg ttgatctgac gtcagcggga
    15481 atgcgtgtgg agatgggctc attgacaacg ctgctgagtg gcggtgtcag ctttgatgtg
    15541 ccggaaggtc tggatttagg gcagccagtg gcaccgaaaa cagctttcgt tttgtatgat
    15601 gatcagaaga gcattcagga ttcgttgtac accgatcaca ttgattatct gatgttcttt
    15661 aaagattcgg tacgcggtct gcaaccggga gctccggtag aattccgggg tattcgcctc
    15721 ggtaccgtaa gcaaagtgcc attctttgcg ccgaatatgc gtcagacatt taacgatgat
    15781 taccgtattc cggtgctgat tcgtatcgag ccagagcggc tgaaaatgca gcttggcgaa
    15841 aatgcggatg ttgttgagca ccttggcgaa ttgttgaaac gtggtttacg cggatcgctg
    15901 aaaaccggaa acctggtcac tggcgcactg tatgttgatc tcgatttcta tccaaatacg
    15961 cctgcaataa ccggtattcg tgaatttaat ggttatcaga ttatcccgac cgttagcggc
    16021 ggcctggcgc aaatccagca acgactgatg gaagcgttgg ataagatcaa caaactgcca
    16081 ttgaatccga tgattgaaca ggcaaccagt acgctttctg aaagtcagcg cacaatgaaa
    16141 aacctgcaaa cgacgctgga tagcatgaac aagatcctcg ccagccagtc gatgcagcag
    16201 ttgccgacgg atatgcagtc aacgttgcgt gaattgaatc gcagcatgca gggcttccag
    16261 cccggctccg cagcctacaa caagatggtg gcggatatgc agcgccttga tcaggtgttg
    16321 cgagaactgc aaccggtgct gaaaacgctc aatgagaaga gtaacgcgct ggtatttgaa
    16381 gcgaaggaca aaaaagatcc agagccgaag agggcgaaac aatgaaaaag tggctagtga
    16441 cgattgcagc actgtggctg gccggatgca gctccggcga aattaataaa aactattacc
    16501 agttacctgt ggtgcagagc ggtacacaaa gtaccgccag ccagggcaat cgcctgttat
    16561 gggtagagca ggtcgctgta cctgactatc tggcggggaa tggtgtggtt tatcaaacca
    16621 gtgatgtgaa gtatgtgatt gccaacaaca acttgtgggc cagcccgttg gatcaacagt
    16681 tgcgcaacac cctggtagcc aacctgagca cgcaactgcc cggctgggtg gttgcctccc
    16741 agcctctggg aagcgcccag gacacgctca atgttaccgt aacggagttt aacggtcgct
    16801 atgatggcaa ggtcattgtc agtggtgagt ggctgttgaa ccaccaggga caactgatca
    16861 aacgtccgtt ccgtctggaa ggagtgcaaa ctcaggatgg ttacgatgag atggttaaag
    16921 tgctggccgg tgtctggagt caggaagccg cttctattgc acaagagata aagcgtctac
    16981 cttaattata aagatttgta aatataaccg tctccggtat gttgcctgag gcggtttttt
    17041 tgtttctaac gtgcggaaaa atttgttcct cttcacattt tttgtacaac cgacatgccc
    17101 gtgtagctca caaatatgac agtggcgtga attttgcgca ttgacggcag ttatgattcg
    17161 cggtattgct taactgtgat tgcacattta gtaatcactg ttttcttttc caccagaaac
    17221 cagtatgagg gaaacgaggc atgaagagac aaaaacgaga tcgcctggaa cgggcacatc
    17281 aacgtggtta tcaggccggc atcgccggac gctcaaaaga aatgtgtccc tatcagacgc
    17341 tgaatcaaag gtcacaatgg ctgggaggct ggcgagaagc catggcggac agggtagtaa
    17401 tggcctgatt ctgtctcttt aaaaagaaac ctccgcattg cggaggtttc gccttttgat
    17461 actctgtctg attataatca gaaggcagac gtatcctgga acagaccgac tttcaggtcg
    17521 ctggcggtat agatcagacg accatcaacc agcacttcgc catccgccag gcccataatc
    17581 agacgacggt taacaatgcg tttaaagtga atacggtagg tcactttttt cgctgtcggc
    17641 agtacctgac cagtgaattt cacttcgcca acgcccagcg cgcggccttt accttcgccg
    17701 cccagccagc cgaggtagaa ccctaccagc tgccacattg cgtccaggcc caggcatccc
    17761 ggcataaccg gatcgccaat aaagtggcat ccgaagaacc acagatccgg attgatatcc
    17821 agttctgctt caacataccc tttgtcgaag ttaccacccg tttcggtcat tttgaccaca
    17881 cggtccatca tcagcatgtt cggtgctggc aattgcgggc ctttagcgcc aaacagttca
    17941 ccgcgaccag aggcaagaag gtcttctttt gtataggatt cgcgtttatc taccatgttc
    18001 tctgtaagcc ttattttatt gaagcacgca ggatagctaa cacgtgtacg ctgaacaagt
    18061 ccgatcagtt cggaataaac cagttcagcc aacgtaatgg ccatggaaaa cggtgacgtc
    18121 cttcctgttg cgatgcttgc gcgatacgtt cctggatggt ttgcatcagc gtcgtttggc
    18181 cttcgccatc ccacaccaga tttaataata acggcagtgc gtcagtcaca tcgtctactg
    18241 cccagatggt gaatttgcct tcttctaccg ctttcaccag ttcactgtga agacttaaat
    18301 ggcgaacgtt agctgtgggg ataatgacac cttgtttccc ggttaactca cgttgctggc
    18361 aaatagcaaa gaagccttcg attttctcat ttaaaccacc gaccggctgg gcgcgaccga
    18421 actgatcgac tgaacctgtg atagcgatac tctgattcac cggcacatcg gcgagggcgc
    18481 ttatcagggc gcagagttca gccatcgagg cactatctcc atcaacttca ctgtatgact
    18541 gctcaaatgt cagcgatgct gagaagggga tctgttgctc aagctgtagt tccgacatca
    18601 ggaacgcttg catgatcatc atccctttcg catggatatt gccgccaagc tccgctttgc
    18661 gttcgatgtc ggtgaattca ccatcgccaa tatgcacaac gcagctaatg cgagaaggtt
    18721 cgccaaaagc gcgtggatga cccggaaatt caatgaccga aagggcgtta atttgcccga
    18781 tgcgttcgcc ttcggtttca atcaggattt gctcctgaag gatctcatcc tgcatacgtt
    18841 cagcgaggaa accttcgcgc cattcacgct gctgcagcat taagtttagc tgctcgccgg
    18901 agaaggtgtc gccatcacac agggaggcga cctctttaca ctggcggagg atccactgcg
    18961 ggctaagcgg aagtgtttct tgttcaccgg tgtagcgtgc tgcttcgcgg ataagtaccg
    19021 gccaggcatc cgctcccggg gcaggtaagt gattatgtct ggcggtaaat gtcacccagc
    19081 gacaccactg ggttactgac tccgcatcga caatctgcag agtatcttca aattcgctat
    19141 aaatagcctg ctctgaaagc tctggctcca tctcctggaa atcagccaat gattcgcgtt
    19201 cgcctaccag aatgactttc agcttcaatg gcatcgaagg cacagagacg gggagagggc
    19261 gcgactcatc aaacgcaacc cagtcaaaac gctcgcggtt aacgatattt ttcagccgca
    19321 tccacagcag aggttgcgcc agcagtgtac gcaaagagat gatgagaata ccgccatttg
    19381 cctgatgcac cagaccaggc tgcagggtaa tgtcgccatt aaactggcgc aggcagccaa
    19441 agagttgctc cgcttctacc cagtcggcag cgacaacttg cgttaaagtc gcaaaattat
    19501 catctgcact cactgcgtga cgtaagcgga tggagtggcc ggaaacctca tagtgaccgc
    19561 ccaccagttg gcctgcatcg ctttgtagcg tacgcgcggc attggcaata agattcagat
    19621 actcagactc ttccggggcc ttcgccagca taaaagagga ggatgctcgc gtatgcagca
    19681 actgctccag cgcaaattgc agccgcggtt gagtatcact gaataaagga tcgttttcgt
    19741 caatcaaatg tggctgagca aatatttcct gatagctatc ggtatcagga accaggtcac
    19801 gccatgcaag tttcgtaatg gtcaaagttg atgtttttta gtctgttgtc aaagccgcga
    19861 ttataccgta accggcacta cagcacacgt agaaagcacc gacaatactc ctggcatggg
    19921 cgttaaagct cacaggatgg agattctttt cttcactggc ctaaaaagct gatattctgt
    19981 aaagagttac acggtaacat tgagatcgct atgaaatatc aacaacttga aaatcttgaa
    20041 agcggttgga aatggaagta tctggtgaag aagcatcgcg aaggggagtt aatcacccgt
    20101 tacatagaag ccagtgccgc ccaggaagcc gtcgatgtgt tgctctcgct ggaaaatgaa
    20161 cccgtgctgg taaatggctg gattgacaag catatgaatc cggaactggt caatcgcatg
    20221 aagcaaacca ttcgggcaag acgaaagcgc cattttaatg cggaacacca gcatacgcgc
    20281 aaaaaatcga tcgatctgga atttattgtc tggcaacgtc tggctggtct tgcgcagcgt
    20341 cgcggtaaaa cgctttctga aacgattgtt cagctgattg aagatgcgga aaacaaagag
    20401 aaatacgcga ataaaatgtc ttctctgaag caggatctgc aggcattgct gggtaaggaa
    20461 taactgacga aagtcagttc aatttactaa aggcaaaaaa accccgcagc agcggggttt
    20521 ttctaccaga cgagaactta agcttgcggc tgagttacaa cgtctttgat acctttaact
    20581 tcgatctcta cgcgacgatc cggagccagg cagtcgatca gtgcagcacg ctgtttcacg
    20641 ttgtcacagg tgttgccagt aaccgggttg gattcgccca taccacgtgc ggagatcttg
    20701 tctgccggga tacctttgga gatcaggtaa tcaacaacag actgagcacg gcgctcggac
    20761 agaccctggt tgtaagcgtc agaaccgatg cggtcggtgt aacccagaac aactacggaa
    20821 ccgtctttcg gatccaggtt gctcagctgg ctgtacagct gatccagagc agcctgacct
    20881 tccggtttca gggttgcttt gttgaagttg aacagaacgt cagacttcag agtgaagtgc
    20941 ttggtctgta cttccggtgc cggagctgga gccggagcaa ctactggagc tgcttcgccc
    21001 tgaccgaaac ggtaggaaac acccaggctc agcatgccgt tgtccggacg agtgccgatg
    21061 gtgtgtgcgt caccgatgtt gttggtccac tggtattcca gacgggtagc gatttcagga
    21121 gtgatcgcgt actcaacacc gccagcgaag accggagaaa cgccggtgtc gtggttttta
    21181 ccataaacgt tggatttagt gtctgcacgc cataccatac cacccagacg agtgtagatg
    21241 tccaggtcgt cagtgattgg gtaacccagt ttagcggtca gttgaacgcc ctgagctttg
    21301 tatgcaccgt tttcaacgct gcctttgtac ggcatacgac ctaaccagtc gtaacccatt
    21361 tcaaagccaa catacgggtt aacctggtaa ccaccaaaag caccagcgcc cagttggttt
    21421 tcatgggtcg ggccattgtt gttgatgaaa ccagtgtcat ggtactggga ccagcccagt
    21481 ttagcaccag tgtaccaggt gttatctttc ggagcggcct gcgctacggt agcgaaacca
    21541 gccagtgcca ctgcaatcgc gatagctgtc tttttcattt tttgcgcctc gttatcatcc
    21601 aaaatacgcc atgaatatct ccaacgagat aacacggtta aatccttcac cgggggatct
    21661 gctcaatatt aactctaccg atatcttcgg cttatgccga gcacccctgg cgatgtaaag
    21721 tctacaacgt agttgaaaac ttacaagtgt gaactccgtc aggcatatga aaaaaaagtc
    21781 ttgtataagg tatgtttaat cttttttgtc agcgacaatt tacagaagag aatcgcggaa
    21841 accgcttcag acaagcctcc gcaaggaaaa ttagtcacga ctgaaagcat tggctgggcg
    21901 acaaaaaaag ttccagga
//
LOCUS       DBFIFI010000062        21236 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.062, whole genome shotgun sequence.
ACCESSION   DBFIFI010000062 DBFIFI010000000
VERSION     DBFIFI010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21236
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.062"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            1..194
                     /locus_tag="N7B39_002241"
                     /pseudo
     CDS             1..194
                     /locus_tag="N7B39_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003844207.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(219..533)
                     /gene="fliE"
                     /locus_tag="N7B39_002242"
     CDS             complement(219..533)
                     /gene="fliE"
                     /locus_tag="N7B39_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HGU2422217.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            748..2406
                     /gene="fliF"
                     /locus_tag="N7B39_002243"
     CDS             748..2406
                     /gene="fliF"
                     /locus_tag="N7B39_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HGU2422218.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSWRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESQAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            2399..3394
                     /gene="fliG"
                     /locus_tag="N7B39_002244"
     CDS             2399..3394
                     /gene="fliG"
                     /locus_tag="N7B39_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HGU2422219.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            3387..4073
                     /gene="fliH"
                     /locus_tag="N7B39_002245"
     CDS             3387..4073
                     /gene="fliH"
                     /locus_tag="N7B39_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HGU2422220.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHKQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            4135..5508
                     /gene="fliI"
                     /locus_tag="N7B39_002246"
     CDS             4135..5508
                     /gene="fliI"
                     /locus_tag="N7B39_002246"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HGU2422221.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            5527..5970
                     /gene="fliJ"
                     /locus_tag="N7B39_002247"
     CDS             5527..5970
                     /gene="fliJ"
                     /locus_tag="N7B39_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HGU2422222.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            5967..7094
                     /gene="fliK"
                     /locus_tag="N7B39_002248"
     CDS             5967..7094
                     /gene="fliK"
                     /locus_tag="N7B39_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HGU2422223.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLISDIVSDAQQANLLIPVDETPPVINDEQSTSTPLTTAQ
                     TMALAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPQVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSSHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            7199..7663
                     /gene="fliL"
                     /locus_tag="N7B39_002249"
     CDS             7199..7663
                     /gene="fliL"
                     /locus_tag="N7B39_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HGU2422224.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            7668..8672
                     /gene="fliM"
                     /locus_tag="N7B39_002250"
     CDS             7668..8672
                     /gene="fliM"
                     /locus_tag="N7B39_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HGU2422225.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            8669..9082
                     /gene="fliN"
                     /locus_tag="N7B39_002251"
     CDS             8669..9082
                     /gene="fliN"
                     /locus_tag="N7B39_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HGU2422226.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            9085..9450
                     /gene="fliO"
                     /locus_tag="N7B39_002252"
     CDS             9085..9450
                     /gene="fliO"
                     /locus_tag="N7B39_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HGU2422227.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            9450..10187
                     /gene="fliP"
                     /locus_tag="N7B39_002253"
     CDS             9450..10187
                     /gene="fliP"
                     /locus_tag="N7B39_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HGU2422228.1"
                     /translation="MRRLLSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            10197..10466
                     /gene="fliQ"
                     /locus_tag="N7B39_002254"
     CDS             10197..10466
                     /gene="fliQ"
                     /locus_tag="N7B39_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HGU2422229.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            10474..11259
                     /gene="fliR"
                     /locus_tag="N7B39_002255"
     CDS             10474..11259
                     /gene="fliR"
                     /locus_tag="N7B39_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HGU2422230.1"
                     /translation="MLQVTSEQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            11549..12172
                     /gene="rcsA"
                     /locus_tag="N7B39_002256"
     CDS             11549..12172
                     /gene="rcsA"
                     /locus_tag="N7B39_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HGU2422231.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKLIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(12216..12404)
                     /gene="dsrB"
                     /locus_tag="N7B39_002257"
     CDS             complement(12216..12404)
                     /gene="dsrB"
                     /locus_tag="N7B39_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HGU2422232.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            12567..12794
                     /gene="yodD"
                     /locus_tag="N7B39_002258"
     CDS             12567..12794
                     /gene="yodD"
                     /locus_tag="N7B39_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HGU2422233.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            13092..13907
                     /gene="yedP"
                     /locus_tag="N7B39_002259"
     CDS             13092..13907
                     /gene="yedP"
                     /locus_tag="N7B39_002259"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HGU2422234.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKMLGLQGLPLIAENGAVIQLAEQWQEIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(13904..15598)
                     /gene="dgcQ"
                     /locus_tag="N7B39_002260"
     CDS             complement(13904..15598)
                     /gene="dgcQ"
                     /locus_tag="N7B39_002260"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HGU2422235.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHIVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRDAV
                     TEFEQHRDEHAWQIELNRRRTLSVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(15769..15951)
                     /gene="yodC"
                     /locus_tag="N7B39_002261"
     CDS             complement(15769..15951)
                     /gene="yodC"
                     /locus_tag="N7B39_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HGU2422236.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(16030..16947)
                     /gene="yedI"
                     /locus_tag="N7B39_002262"
     CDS             complement(16030..16947)
                     /gene="yedI"
                     /locus_tag="N7B39_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HGU2422237.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQVH"
     gene            17120..18040
                     /gene="yedA"
                     /locus_tag="N7B39_002263"
     CDS             17120..18040
                     /gene="yedA"
                     /locus_tag="N7B39_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HGU2422238.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(18029..18499)
                     /gene="vsr"
                     /locus_tag="N7B39_002264"
     CDS             complement(18029..18499)
                     /gene="vsr"
                     /locus_tag="N7B39_002264"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HGU2422239.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLKDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(18480..19898)
                     /gene="dcm"
                     /locus_tag="N7B39_002265"
     CDS             complement(18480..19898)
                     /gene="dcm"
                     /locus_tag="N7B39_002265"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HGU2422240.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(19965..20660)
                     /gene="yedJ"
                     /locus_tag="N7B39_002266"
     CDS             complement(19965..20660)
                     /gene="yedJ"
                     /locus_tag="N7B39_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HGU2422241.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQSSSILAAEETRRLLREEFVQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDAEDPLAQ
                     HRPLDDKRYALDHFQTKLLKLPQIMQTVRGKQLAQHNAHFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPAG"
     gene            complement(20700..20980)
                     /gene="drpB"
                     /locus_tag="N7B39_002267"
                     /pseudo
     CDS             complement(20700..20980)
                     /gene="drpB"
                     /locus_tag="N7B39_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
BASE COUNT         5111 a         5632 c         5477 g         5016 t
ORIGIN      
        1 cagaggttac tgttgcgtct gtggtcgtaa caacatccga cgtggtgatg tgtcaaatga
       61 gacactctga tgttcagggg gtttatcgtg tatacggatc gtggatggca gaaaactttc
      121 aggatcaggt atccatttca aaccagataa tgagtaagtt tgccccatcc atgccccacg
      181 cggtgagatc ggatgtcatt aacaacaggt tacacaacct atacctgcat gctcattact
      241 tcctgatatg ccgccaccag cttattacgc acctgaatcc ccatttgcat agaaactgag
      301 gctttttgca tatcggtcat cacatcgttt aacgccacgc cgggttcacc gagagtgaat
      361 ttttctgcct gcgtgcgggc ggctgtttgt gtatcgctta tgcgatcgag cgcggcgtgc
      421 agctgcccgg caaaactaat ggtcggttgc ggcagtgatt cctgcgcacg cgcactcatc
      481 gccgtagcct gtaactggct gataacccct tcaatcccct gtatcgctga cattttcatc
      541 tcctggataa tttctggtag caaagcctac cagtaagtca ataagacaaa ggcgctaaat
      601 agcaacaaaa aaacgggttt attggcggat agaaaaaaac gaaagcacaa ataatgggag
      661 cgtcaatttt tcgagtttgc tgacccggga gtgagtcttg ttccactttg ccaataacgc
      721 cgtccataat cagccacgag gtgcgcgatg aatgcgactg cagcccagac aaaatctctt
      781 gagtggctta atcgcctgcg tgcgaatccg aaaattccat tgattgttgc cggttccgcg
      841 gcagtggcgg tcatggtcgc actgatcctg tgggcgaaag cccccgacta ccgcacatta
      901 ttcagcaatc tttccgatca ggatggtggc gcaattgtca gccaactgac gcaaatgaat
      961 attccttacc gcttcagcga agccagcggc gctattgaag ttccggcaga taaagttcac
     1021 gagctgcgcc tgcgcctggc gcaacaaggt ttgcccaaag gcggcgcggt cggtttcgaa
     1081 ctactcgatc aggaaaagtt tggtatcagc cagttcagcg aacaggtgaa ttatcagcgg
     1141 gcgctggaag gcgagctttc tcgtaccatc gaaactatcg gcccggtaaa aggggcgcgc
     1201 gtacatctgg caatgccgaa accgtcttta ttcgtccgtg aacaaaaatc cccttctgca
     1261 tcggtgacgg taaatctgtt acccggccgc gcactcgatg aagggcaaat tagcgccatt
     1321 gtgcatctgg tttccagcgc cgttgctggt ctgccgccgg gaaacgtcac gctggtggat
     1381 cagggcggac atctgttaac ccagtccaat accagctggc gcgatcttaa tgacgctcaa
     1441 ttgaaatatg ccagcgatgt cgaaggccgt attcagcggc gtattgaagc aatcctgtcg
     1501 cctattgttg gtaacggtaa tattcacgcc caggtcacgg cgcaactgga cttcgccagt
     1561 aaagaacaaa cggaagaaca gtatcgccct aatggtgatg aatctcaggc ggcgcttcgt
     1621 tcacgccagc ttaatgagag cgagcaaagc ggttccggtt atccgggcgg cgtaccgggg
     1681 gcgttgtcga atcaaccggc tcctgcgaat aacgcgccaa tcagcacgcc tccggcaaat
     1741 caaaataacc gccagcagca ggcgagcacc accagcaata gtgggccgcg tagcacacag
     1801 cggaatgaaa ccagtaacta cgaagtcgat cgcactattc gtcataccaa aatgaacgtg
     1861 ggcgatgtgc aacgtctgtc agtcgcggta gtggtgaatt acaaaacctt gccagacggt
     1921 aaaccattgc ctctcagcaa cgaacagatg aaacaaattg aagatctgac ccgcgaggcg
     1981 atgggctttt ctgaaaaacg cggtgactcg ctcaatgtcg ttaactcgcc gttcaatagt
     2041 agtgacgaaa gcggcggaga actgccgttc tggcaacagc aagcgtttat cgatcagtta
     2101 ctggctgccg gtcgctggtt gctggtactg ctggtggcgt ggctgctgtg gcgtaaagcg
     2161 gtacgcccgc agctaacacg tcgcgctgag gcgatgaaag ctgtacagca acaggcgcag
     2221 gcccgcgagg aagtggaaga tgcggtggaa gtccgcctga gcaaagacga acaactccaa
     2281 caacggcgcg ctaaccaacg tctgggggca gaagtcatga gccagcgtat ccgtgaaatg
     2341 tctgataacg atccgcgcgt ggtggcgctg gtcattcgcc agtggataaa taacgatcat
     2401 gagtaacctg acaggcaccg ataaaagcgt catcctgctg atgaccattg gcgaagaccg
     2461 ggcggcagaa gtgttcaagc acctctccca gcgcgaggtg caaaccctga gcgctgcaat
     2521 ggcgaacgtc acgcagatct ccaacaagca gctaaccgat gtgctggcgg agtttgagca
     2581 agaagctgaa cagtttgccg cactgaatat caacgccaac gattatctgc gttcggtatt
     2641 ggtcaaagct ctgggtgagg aacgtgccgc cagcctgctg gaagatattc tcgaaactcg
     2701 cgataccgcc agcggtattg aaacgctcaa ctttatggag ccgcagagcg ccgccgatct
     2761 gattcgtgat gagcatccgc aaattatcgc caccattctg gtgcatctga agcgcgccca
     2821 ggccgccgat attctggcgt tgttcgatga acgtctgcgc cacgacgtga tgttgcgtat
     2881 cgccaccttt ggcggcgtgc agccagccgc gctggcggaa ctgaccgaag tactgaatgg
     2941 cttgctcgac ggtcagaatc tcaagcgcag caaaatgggc ggcgtgagaa cggcagccga
     3001 aattatcaac ctgatgaaaa ctcagcagga agaagccgtt attaccgccg tgcgtgaatt
     3061 cgacggcgag ctggcgcaga aaatcatcga cgagatgttc ctgttcgaaa atctggtgga
     3121 tgtcgacgat cgcagcattc agcgtctgtt gcaggaagtg gattccgaat cgctgttgat
     3181 cgcgctgaaa ggggccgagc agccactgcg cgagaaattc ctgcgcaata tgtcgcagcg
     3241 tgccgccgat attctgcgcg acgatctcgc caaccgtggt ccggtgcgtc tgtcgcaggt
     3301 ggaaaacgaa cagaaagcga ttctgctgat tgtgcgccgc cttgccgaaa ctggcgagat
     3361 ggtaattggc agcggcgagg atacctatgt ctgataatct gccctggaaa acctggacgc
     3421 cggacgatct cgcgccacca caggcagagt ttgtgcccat ggtcgagtcg gaagaaacca
     3481 tcattgaaga ggctgaaccc agccttgagc agcaactggc gcaactgcaa atgcaggccc
     3541 atgagcaagg ttatcaggcg ggtattgccg aaggtcgcca gcaaggtcat aagcagggct
     3601 atcaggaagg actggcccag gggctggagc aaggtctggc agaggcgaag tctcaacaag
     3661 cgccaattca tgcccggatg cagcaactgg tcagcgaatt tcaaactacc cttgatgcac
     3721 ttgatagtgt gatagcgtcg cgcctgatgc agatggcgct ggaggcggca cgtcaggtca
     3781 tcggtcagac gccaacggtg gataactcgg cactgatcaa acagatccaa cagttgttgc
     3841 agcaagaacc gttattcagc ggtaaaccac agctgcgcgt gcacccggat gatctgcaac
     3901 gtgtggatga tatgctcggc gctaccttaa gtttgcatgg ctggcgcttg cggggcgatc
     3961 ccaccctcca tcctggcggc tgtaaagtct ccgccgatga aggcgatctc gacgccagtg
     4021 tcgccactcg ctggcaagaa ctctgccgtc tggcagcacc aggagtggtg taatgaccac
     4081 gcgcctgact cgctggcaag aactctgccg tctggcagca ccaggagtgg tgtaatgacc
     4141 acgcgcctga ctcgctggct aaccacgctg gataactttg aagccaaaat ggcgcagttg
     4201 cctgcggtac gtcgctacgg gcgattaacc cgcgctaccg ggctggtgct ggaagccacc
     4261 ggattacaat tgccgctcgg cgcaacctgt gtcattgaac gccagaacgg cagcgaaacg
     4321 cacgaagtag aaagcgaagt cgttggcttt aacggtcaac ggctgttttt aatgccgctg
     4381 gaggaagtgg aaggtgtcct gcccggcgcg cgggtttatg ccaaaaacat ttcggctgaa
     4441 ggactgcaaa gcggcaagca gttgccgctc ggtccggcgt tattaggtcg cgttctggac
     4501 ggcagcggta aaccgctcga tggcctgccc tcccccgata cgacggaaac cggtgcgctg
     4561 attaccccgc catttaaccc gttgcaacgt acaccgattg aacatgtgct ggacaccggc
     4621 gtgcgcccaa tcaatgccct gcttaccgtt gggcgtgggc agcgtatggg gctgtttgcc
     4681 gggtccggcg ttggtaaaag tgtgctgctg gggatgatgg cacgttacac ccgcgccgat
     4741 gtcattgtcg tgggtttgat tggtgaacgt gggcgcgaag taaaagattt tattgagaat
     4801 atcctcggtg ccgaagggcg tgcacgatca gtggtgattg ccgctccggc ggatgtgtct
     4861 ccgctcctgc gaatgcaagg tgccgcctat gccacgcgca ttgccgaaga ttttcgcgat
     4921 cgtggtcagc atgtgttgct gattatggac tccctcaccc gctacgcgat ggcccagcgt
     4981 gaaattgcgc tggcgattgg cgaaccaccc gccaccaaag gttatccacc gtcggtgttt
     5041 gccaaattac cggcactggt cgagcgtgcc ggaaatggca ttagcggcgg cggctcgatt
     5101 accgcgtttt ataccgtgct tactgaaggc gatgaccagc aggaccccat tgcagactcc
     5161 gcgcgggcga tcctcgacgg tcacattgtg ctgtctcgcc gactggcgga agccgggcac
     5221 tatccggcta tcgatattga agcgtcgatc agccgcgcaa tgacggcgtt gatcagtgag
     5281 caacattacg cgcgagtgcg caccttcaaa cagctgttgt cgagttttca gcgtaaccgc
     5341 gatctggtta gcgtcggcgc gtatgccaaa ggcagcgatc cgatgctcga caaagccatc
     5401 gccctgtggc cgcagctgga gggctatttg caacaaggca tttttgaacg cgcggactgg
     5461 gaagcgtctc tccaggggct ggagcgtatt ttcccgacag tgtcataacc caggagataa
     5521 cggcagatgg cagaacatgg cgcgctggcg accctgaaag atctggcaga aaaagaggta
     5581 gaggatgccg cgcgcctgct gggtgaaatg cgtcgcgggt gtcagcaggc ggaagaacag
     5641 ctcaaaatgc tgatcgatta tcagaatgaa tatcgcaata acctcaacag cgatatgagt
     5701 gccgggatga ccagcaaccg ctggatcaac tatcagcagt ttatccagac gctggaaaaa
     5761 gccattactc agcatcgcca gcaacttaat cagtggacgc agaaagttga cattgccctg
     5821 aacagctggc gagaaaagaa acaacgtttg caggcctggc agacactgca ggaacggcaa
     5881 tccacggcgg cactgcttgc agaaaaccgc ctcgatcaga aaaagatgga tgagttcgcc
     5941 cagcgcgccg ccatgaggaa acctgaatga ttcgcttagc gcccttgatt accgccgacg
     6001 ttgacaccac cacattgcct ggcggcaaag ccagcgatgc tgcacaagat tttctcgcgt
     6061 tgttgagcga agcattagca ggcgagacaa ctaccgacaa agcggccccc cagttgctgg
     6121 tggcaacaga taagcccacg acaaaaggcg agccgctgat cagcgatatt gtttccgacg
     6181 cgcaacaagc taatttactg atccctgtgg atgaaacacc gcctgtcatc aacgacgaac
     6241 aatccacatc aacaccgtta accaccgctc agacgatggc gttggctgcg gtggctgaca
     6301 aaaatacgac aaaagacgaa aaagcggatg atctgaatga agacgtcacc gcaagcctga
     6361 gcgccctttt tgcgatgttg ccgggttttg acaatacgcc caaagtgact gatgtgccgt
     6421 caaccgtgtt accggcagag aaaccaacgc tcttcacaaa actgacttct gcgcaactca
     6481 caacagcaca gcctgatgat gcccccggca caccagctca gccattaaca ccgcaggtag
     6541 cagaagccca gagtaaagcg gaagtcatca gcacgccttc gccggtgact gctgccgcca
     6601 gcccgctaat cactccacac cagacacagc cactgcccac cgtcgccgcg cctgttttga
     6661 gtgcaccgct gggttctcac gaatggcaac aatcattaag ccagcatatt tcgctgttca
     6721 cccgccaggg gcaacaaagt gcagagttgc gtctgcaccc gcaggattta ggtgaagtgc
     6781 aaatctccct caaagtggat gataaccagg cgcaaatcca gatggtttca tcgcatcagc
     6841 atgtacgcgc cgccctggaa gcagcgctgc cggtactgcg tacgcagctg gccgaaagtg
     6901 gcattcagtt agggcaaagc aacatcagtg gcgaaagctt tagtggtcag cagcaggccg
     6961 cttcccaaca acagcaaagc caacgcacag taaaccatga acctctggcg ggggaagacg
     7021 acgatacgct tccggttccc gtctctttac aagggcgcgt aacaggcaac agcggcgttg
     7081 atattttcgc ctaacgtcag aggtagcacc gtaatccgcg tcttttcccc gctttgttgc
     7141 gctcaagacg caggataatt agccgataag cagtagcgac acaggaagac cgcaacacat
     7201 gactgattac gcgataagca agaaaagcaa gcgatcgctt tggatcccga ttctggtatt
     7261 cattaccctc gcggcctgtg ccagcgcagg ttacagctac tggcattcgc atcaggttgc
     7321 tgctgacgac aaagcgcagc aacgcgtcgt gccctccccg gtcttctatg cgctggatac
     7381 ctttacggtc aatctgggcg atgcggatcg cgtgctttat atcggcataa ccctgcgcct
     7441 gaaagatgaa gctacccgct cgcggctgag tgagtatttg ccggaagtcc gtagccgttt
     7501 gctgttactg ttttcgcgtc aggatgctgc cgtactggcg acagaagaag gcaagaaaaa
     7561 cctgattgcc gagattaaaa ccacactttc caccccgctt gttgccgggc aaccgaaaca
     7621 ggatgtcacc gacgtgctgt ataccgcttt tattctgcga taacgacatg ggcgatagta
     7681 ttctttctca agctgaaatt gatgcgctgt tgaatggtga cagcgaagtc aaagacgaac
     7741 cgacagccag tgttagcggc gaaagtgaca ttcgtccgta cgatccgaat acccaacgac
     7801 gggttgtgcg cgaacgtttg caggcgctgg aaatcattaa tgagcgcttt gcccgccatt
     7861 ttcgtatggg gctgttcaac ctgctgcgtc gtagcccgga tataaccgtc ggggccatcc
     7921 gcattcagcc gtaccatgaa tttgcccgca acctgccggt gccgaccaac ctgaacctta
     7981 tccatctaaa accgctgcgc ggcactgggc tggtggtgtt ctcaccgagt ctggtgttta
     8041 tcgccgtgga taacctgttt ggcggcgatg gacgcttccc gaccaaagtg gaaggtcgcg
     8101 agtttaccca taccgaacag cgcgtcatca accgcatgtt gaaactggcg cttgaaggct
     8161 atagcgacgc atggaaggcg attaatccgc tggaagttga gtacgtgcgt tcggaaatgc
     8221 aggtgaaatt taccaatatc accacctcgc cgaacgacat tgtggttaac acgccgttcc
     8281 atgtggagat tggcaacctg accggcgaat ttaatatctg cctgccattc agcatgatcg
     8341 agccgctacg ggaattgttg gttaacccgc cgctggaaaa ctcgcgtaat gaagatcaga
     8401 actggcgcga taacctggtg cgccaggtgc agcattcaca gctggagctg gtcgccaact
     8461 ttgccgatat ctcgctacgc ctgtcgcaga ttttaaaact gaaacccggt gacgtcctgc
     8521 cgatagaaaa acccgaccgc atcatcgccc atgttgacgg cgtcccggtg ctgaccagtc
     8581 agtacggcac tctcaacggt cagtatgcgt tacggataga acatttgatt aacccgattt
     8641 taaattctct gaacgaggaa cagcccaaat gagtgacatg aataatccgg ccgatgacaa
     8701 caacggcgca atggacgatc tgtgggctga agcgttgagc gaacaaaaat caaccagcag
     8761 caaaagcgct gccgacgcgg tgttccagca atttggcggt ggtgatgtca gcggaacgtt
     8821 gcaggatatc gacctgatta tggatattcc ggtcaagctg accgtcgagc tgggccgcac
     8881 acgaatgacc atcaaagagc tgttgcgtct gacgcaaggg tccgtcgtgg cgctggacgg
     8941 tctggcgggc gaaccactgg atattctgat caacggttat ttaatcgccc agggcgaagt
     9001 ggtggtcgtt gccgataaat atggcgtgcg gatcaccgat atcattactc cgtctgagcg
     9061 aatgcgccgc ctgagccgtt agtgatgaat aaccacgcta ctgtgcaatc ttccgcgccg
     9121 gtttctgctg cgccactgct gcaggtgagc ggcgcactca tcgccattat tgccctgatc
     9181 ctcgctgctg cctggctggt aaaacggttg ggatttgccc cgaaacgcac tggcgttaac
     9241 ggtctgaaaa ttagcgccag tgcttcgctg ggcgcgcgtg aacgggttgt ggtggtcgat
     9301 gtggaagatg cacggctggt gctcggcgtt accgcagggc aaatcaatct gctgcataaa
     9361 cttccccctt cagcaccaac agaagagata ccgcagaccg attttcagtc ggtcatgaaa
     9421 aatttgctta agcgtagcgg gagatcctga tgcgtcgttt attgtctgtc gcacctgtcc
     9481 ttctctggct gattacgccc ctcgccttcg cgcaactgcc gggtatcacc agccagccgc
     9541 tgcctggcgg tggacaaagc tggtcgctcc cggtgcagac gctggtgttc atcacctcgt
     9601 tgacgtttat tccggcaatt ttactgatga tgaccagttt cacccgcatc atcattgttt
     9661 ttggtttatt gcgtaacgcg ctgggaacac cctccgcgcc acctaaccag gtattgctgg
     9721 ggctggcact gtttttgacc ttttttatta tgtcaccggt gatcgacaaa atttatgtcg
     9781 atgcgtacca gccattcagc gaagagaaaa tatcaatgca ggaggcgctg gaaaaagggg
     9841 cgcagccgct gcgtgagttt atgctgcgtc agacccgtga ggcagattta gggttgtttg
     9901 ccagactggc gaataccggc ccgttgcagg gacctgaagc cgtgccgatg cgcattttgc
     9961 tcccggccta cgtgaccagc gagttgaaaa ccgcatttca gataggcttc acgattttca
    10021 tccctttttt gattatcgac ctggtgatag ccagcgtgtt gatggcattg gggatgatga
    10081 tggttccccc cgccaccatt gctctgccct ttaaactgat gctgtttgta ctggtggatg
    10141 gctggcaatt gctggtcggt tcgctggcgc agagctttta cagctagaga ggcaaaatga
    10201 cacctgaatc ggtcatgatg atggggactg aagcgatgaa agtcgcgctg gcactggctg
    10261 ccccgctatt gttggtagcg ttggtcacgg gccttatcat cagtattttg caggccgcca
    10321 cgcagattaa cgaaatgacg ctgtcgttta ttccgaaaat catcgccgta tttatcgcca
    10381 ttattattgc cggaccgtgg atgctcaatc tgttgctgga ttacgtccgc accttgttca
    10441 ctaacctgcc gtatatcatc gggtagccgt actatgttgc aggtgacaag cgaacaatgg
    10501 ctatcctggt taagcctgta cttctggccg ttactgcgcg tgctggcgct gatctccacc
    10561 gcgccgattc tgagcgaacg cagcgtaccg aaacgggtaa aactgggtct ggcaatgatg
    10621 atcacgttcg ccattgcccc atcattacct gccaacgatg ttcctgtttt ttcgttcttt
    10681 gctctgtggc tggccgtgca gcagatcctg atcggcattg cgcttggttt taccatgcaa
    10741 tttgcctttg ccgctgtgcg aaccgctggc gaaattatcg gtctgcaaat ggggctgtca
    10801 tttgcgacgt ttgtcgatcc ggccagccat cttaatatgc ccgttttagc gcgtatcatg
    10861 gatatgctgg cgttactgct gttcctgaca tttaacggtc atttatggtt gatttcactg
    10921 ctggtcgata cctttcacac cctgccgatt ggtggcgaac cgttgaacag caatgcgttt
    10981 ctggcactca ccaaagcagg gagtttgatt ttccttaacg ggctgatgct ggcgttaccg
    11041 ctcattactc tgctgctgac actgaatctg gcattaggtt tacttaatcg tatggccccg
    11101 caattatcca tttttgttat tggatttcca ttaactctga ctgtcggcat ctctttaatg
    11161 gcggcattaa tgccgttaat tgcgcctttt tgcgaacatt tattcagtga aatttttaat
    11221 ttgctggctg atattattag tgaattgcca ttaatataat tccgtaacgt ttatcatgtt
    11281 atcctaagga ttatccgaaa aataatacct acgaacatct tccaggatac tcctgcagcg
    11341 aaatatttgt tttaagctca ctcacatatc gcaacattta ctttacttta agacaattcc
    11401 aggcaaatta tacaacactt tacgggatag taagtccgcc tgaaaaatcg cgagagtggc
    11461 gcattaggtg acccatgttg ttccgtttag tcatgatgaa atattcaggt aaggggaatt
    11521 atcgttacgc attgagtgag ggtatgccat gtcaacgatt attatggatt tatgtagtta
    11581 cacccgacta ggtttaaccg ggtatctgtt gagtagaggg gttaaaaaaa gagaaatcaa
    11641 cgacattgaa accgttgatg accttgccat agcttgtgat tcacagcgcc cttcagtggt
    11701 gtttattaat gaggactgtt tcatccacga tgcttctaac agtcagcgta tcaagctcat
    11761 cattaatcaa catcccaata cgttatttat cgtttttatg gcaattgcca atgttcattt
    11821 tgatgaatat ctattggtca gaaaaaattt attgatcagt tctaaatcga ttaaaccgga
    11881 atctctcgac gatatccttg gcgatattct gaaaaaagag acaacgataa cctcgttttt
    11941 aaatatgccg acgttatcat tgagccgaac cgaatcgagt atgttgcgaa tgtggatggc
    12001 aggtcaggga accattcaaa tctccgacca aatgaatatc aaagccaaga ccgtttcatc
    12061 gcataaaggt aatattaaac gtaagatcaa aacgcataat aaacaggtta tctaccatgt
    12121 cgtccgactg acggataatg tgactaatgg tatttttgtc aacatgcgct aacacattct
    12181 gactggtggt ttcccaccag tcaggctgaa ttagattact ctgctttctc cacaaagata
    12241 ccgtcctgat gccctgcttc attaaagaac cagatgccga gcgggtagtc ttccagcgaa
    12301 accaggtaca ttgtgccttc actaaactcc tcaactgcca gtaccacgcc aggacgacgc
    12361 ggaccgccat ccgttttgac tgttacccga tcattcacct tcatcgtttt cctcctgtgg
    12421 ctttgtgcca gtgtagaaca atttcgtttt tctggcagcg ccaggcgcgc gcgagtgctg
    12481 attttctcga cggtctatac ttaagagatg ccaggcggac ttaacgactg gcggcaacaa
    12541 cagagtaacg gttgcgagga aagatgatga aaaccgcaaa agagtacagc gataccgcaa
    12601 aacgtgaggt cagcgtcgat gtcgatgccc tgctggcggc gatcaatgaa attagcgaaa
    12661 gcgaagttca tcgcagccag aacgattctg aacacgttag cgtcgatgga cgtgaatatc
    12721 atacatggcg tgaattggcg gatgccttcg aactggatat tcatgacttc agcgtctctg
    12781 aagtgaatcg ttgaatgcac aataaaaaaa tcccgaccct gagggggtcg ggatgaaact
    12841 tgcttaagca agaagcactt aaaaaattcg ttacaccagg aaatctgatg tgttcatcac
    12901 cttatccgca atttttttcg ctgacaagaa aatattcgct atatgaatga ttaacgctac
    12961 ttatgaatag aaatgtgacg cacgtcaccc cttggtggct ctggcgtcag cgggatcgct
    13021 cccgaaaaaa tcatctttga taatttgtgc tgttttgaga atcatcctgg taaggggagt
    13081 actgcgcgac tatgttttca attcaacaac cactactggt ttttagcgat cttgatggca
    13141 ccctgctgga cagtcatagt tatgactggc aaccggcagc cccctggctc agccgtttac
    13201 gcgaagcaaa tgttcccgtc attctctgta gcagtaaaac atcagcggaa atgctgtact
    13261 tgcaaaaaat gttggggcta caaggtttac cgctgattgc agagaatggc gcagtgatcc
    13321 agcttgctga acaatggcag gagatagacg gttttccacg catcatctca ggtattagcc
    13381 atggcgaaat cagccaggtt ttaaatacgc tacgcgagaa agaacatttt aaattcacga
    13441 cttttgatga tgtcgacgat gcaaccatcg ccgaatggac gggattaagc cgtagccagg
    13501 cggcgctgac gcagcttcat gaggcgtcgg taacgctaat ctggcgcgac agtgacgagc
    13561 gtatggcaca atttaccgct cgtctgaacg aactgggctt acagtttatg caaggtgcgc
    13621 gcttctggca cgtcctggat gcctctgccg gaaaagatca ggctgccaac tggattatcg
    13681 cgacctatca acaattgtca ggcaaacgcc caaccacact tggcctgggc gatgggccaa
    13741 acgatgcgcc cttactggag gtaatggatt acgcggtgat tgtgaaaggg ctaaaccgtg
    13801 aaggggtgca tctgcatgat gaggatccgg cccgcgtctg gcgaacgcag cgtgaaggac
    13861 cggaaggatg gcgtgaaggg ctggaccatt ttttctccgc ccgttaagcg ttatcgctcg
    13921 cgaatacccg attgcgccca gcctgtttag cgagataaag ccgacggtcg gccagtgact
    13981 ggagttgttc aaaatcataa tcaccggttt cctcgctgct acttaccccc aacgaggcac
    14041 tgatgcgtat cgtcgtactc ttagcaatca acatctcttt ttcatttaac ttcaggcgaa
    14101 tacgttctgc gacttccgca gcctccgtca gactcgcgcc tggcagaatc acacaaaact
    14161 cctcaccacc gacccgcccg gcaacgtcct gcgcacgcaa ggaactgcta attaatccgg
    14221 cagcatgaga aagaacacgg tcgcccgcct gatgaccaaa gcggtcatta atcgctttaa
    14281 aatggtcaag atcgacctga atgacagaaa aaggatgttg gtgcgtctga cacaatttcg
    14341 cgagcggacg ggctttttcg aacagtgcgc cacggttata taaacgcgtt aaggtgtcgt
    14401 gccacgcctg ccactgcaac gagctttgca gaacatacat gttgctgacc atccggcgaa
    14461 tcacatacca ggagatgagt aacatggtgg taaagagcgc ccacagcagg gttaatgcaa
    14521 tgctgatact gccgaaatcg ccgcgcacgc cttcgcttaa cgtatggacg cgcaccagca
    14581 caccgtcgaa atggtccaga cgttcccagc taacatagcg actgtccata cgaatgccgc
    14641 cccgcgtgtc atgttccatt gcctgcgcca gcaaggccag ttcacgagga tcaaaaatat
    14701 tccctgttgg atggtcagga ttggaagagg tcaaaaatct cagcttactg tcatagagct
    14761 gatactcacc atcgaggttt ttatcgatgg cgtttcttaa aaattgctgc atagtacgca
    14821 cgggaatact catccccagc acgccatacc agtagttatt actgtctacc ggaacactga
    14881 cggtaacctg cggttcagta ttgctggcgt gttccggttg cgaagtgaac cagcgtaccg
    14941 cgcggtgacg attttctcgt tgtgaatggc cgataaacca gggttgggtg acatagccgt
    15001 aataacgcgt cgggacattg cgcgtaaaca aggtcggctg cgtcgaaacg taaaatccgg
    15061 cacgcgagac atacatcgcc tgttctacca tcgacgagga gttgtgtgcc agtcgcagca
    15121 agtaaccaac ttccagtgca gcggtaattt cattgtcgag gctttcattt tcgcgagaca
    15181 ggagattccc ctcgctgact aatgcatccg atacgccatt gactgacagg gtgcgtcgtc
    15241 ggttgagttc gatttgccag gcgtgctcgt cgcgatgctg ttcgaactcg gttacagcat
    15301 cacgcagaga ggtgaaatcc agtggcgcta cgagagcttc gcgcatgcca ttacgcaaaa
    15361 agatcagttt gtcgacgtta tactgcaaat gcttatcgag cgcgttggct acgttttcca
    15421 gatgattacg ctggctggag atataggcat cttccagcac cactacttca cgccaggtca
    15481 gcaaggtgga aaaaagcaat accacgataa agcagagatt aacgatatga ccaggcccta
    15541 ggcggcgtgc gagttttttc aaccagctct ggttttccat ttttgtctcg tgctgcaccc
    15601 tgacgactcc ccacctgctt ttttatgatt ctggcataac ctggcgatag cgtaccgcaa
    15661 aacagcgata attttataat ggtggcatga taagacgcga ctatcattca ttgcaggcat
    15721 tggcacacca ggccggatgt gatccggcct ggtcttaatg ataccctttc aaacttcttc
    15781 cgcagaacga ctcccctccc ctggaacaag ctcggtttca tgaaaagctt cccgcttgac
    15841 cccgtaaccg tcataccaac gacactcaac cataccgctg gagtatccag tgacaatcat
    15901 ccgcgggccg ccctctttaa ccgtaacttc ctcactaacc ataaagctca tactcgcctc
    15961 cttttttcga gtgaaacttg ttcaccttag ttgaagatgg cgaattttgc ataatagcct
    16021 gtgcgagtat taatgtacct gaccgcgcat tttcgctacg gctttcactc ccagcaccac
    16081 gatgccgccg atgataaatc caagaatcag atttaaaaca gtcggtaata tcatcgccac
    16141 cactgcactt tgctgcccgg cgaaatgttc aatggcgtga tgcagcggcg caataccatg
    16201 taccacaatc ccgccgccga cgaggaacat cgccagcgtg ccgacaatcg ataacgcttt
    16261 catcagccag ggcgcgataa tcaataatcc tttacctaat gcctgcatca gcgcgctgga
    16321 tttttccgcc agccaatacc ccaggtcatc aatcttaacg ataaccccta ccagaccgta
    16381 gacgcccacg gtcactacca gcgcgatgcc tgaaagcacc agcacctgat taagcaacgg
    16441 cgcttcggcc acaatcccca gcgtgatggc gacgatttcc gcagacaata taaaatcggt
    16501 acgaatcgcc ccttttattt tgtccttttc aaacttcagc ggatcctgcg ccgccagctt
    16561 ctccagacgc tgctggctct gcgccggatc ttctttatgt ttacgcgcct ccagcatatg
    16621 cagcactttc tctacccctt caaagcagag aaacgcgcca ccgatcatca acaatggcgt
    16681 aatcgcccac gggataaacg cactgatgat cagcgccagt ggcaccagaa tcactttatt
    16741 aatcagcgat cctttcgcca cgccccagac cacgggaagt tcccggttgg cccgcacgcc
    16801 tgaaacttgt tgcgcattga gcgataagtc atcccctaat acaccggcgg ttttcttcgc
    16861 cgccagtttg cccatcacgg agatatcgtc cagcagtgtg gcgatatcat cgagcaacgt
    16921 cagtaaacta ctcccggcca aaagcattcc ctctcatttt tattgttgaa gggtgtaagt
    16981 atgaagcaaa aacggcagct ctgatacttg cttcacaggt caacattttt ttaacaatca
    17041 agtgcaatta tatctctcgc cagcagaatg attttcacgt ttactatgaa ccaccttttt
    17101 atttccaccg tgagggatta tgcgtttccg ccagttgtta ccgctttttg gcgcgctgtt
    17161 tgcgttgtat atcatttggg gctcaaccta ttttgtcatt cggattggcg tggaaagctg
    17221 gcctccgtta atgatggcgg gtgttcgatt cctggcagcc ggtattttat tgctggcatt
    17281 tttactactg cgcggacaca aactcccccc gctacgtccg ctgctcaatg ccgcgctgat
    17341 tggcctgtta ttgctggctg tcggtaatgg catggtgacg gttgccgaac atcaaaatgt
    17401 tccttccggc atcgccgccg tagtggttgc aaccgtgccc ctctttaccc tgtgcttcag
    17461 ccgcctgttt ggcattaaaa cgcgcaaact ggaatgggtg ggtattgcca ttgggcttgc
    17521 cggaatcatc atgctcaata gcggtggaaa tttaagcggc aatccgtggg gcgcgattct
    17581 gattttaatc ggctcgatta gctgggcgtt tggctcagtt tatggctcgc gcattacctt
    17641 acctgtaggg atgatggcgg gtgcgattga gatgctggcg gcaggcgtgg tgttaatgat
    17701 cgcgtcgatg attgcgggtg aaaaactgac ggcgctccct tccctttcag gcttccttgc
    17761 ggtcggctat ctggcgctgt ttggttcgat tatcgccatc aacgcttata tgtatttaat
    17821 ccgtaatgtc agtccggctc tcgccaccag ctacgcttac gttaacccgg tggtcgcggt
    17881 cttgctgggt acgggactgg gtggagaaac actgtcgaag attgaatggc tggcgctcgg
    17941 cgtaattgtc ttcgcggtgg tactggtcac gttgggaaaa tatctctttc cggcaaaacc
    18001 cgtagttgct ccagttattc aggacgcatc aagcgagtaa atgtatcccc tgcgtgtcga
    18061 tctgcgcgct ggctccttcg ccgcagatcc actcttccag acgctcggta agcgcttcat
    18121 ccttcagctt ctcgcgccca cgtaacgcgc actcccagac aatcaatacg cgccagccga
    18181 gttcctgcaa gcgactgata tcgcggcgat cgcgctcaac atttttacct atcttctcca
    18241 gccagaactc ggttcgagtc gcaggcactt taaacagata gcagtgatga tgatgccaga
    18301 agcagccatg ggtaaatatc acgcagcgat attcatcaac gacaaaatcc gggcgtccgg
    18361 gcagactggc gtcctgaacg cgaaacgcca ggccctgccc ggttaacaga ctggcgaggc
    18421 gcttctcaat cgccgtatca cgcgtggcaa tcgcgcgcat atttttgctg cgagtggcct
    18481 tatcgtgaac gtcggccatg ttgtgcctct tgctgacgca acgccaccgc ctgtttgatt
    18541 tttggctcaa gcagttttgc caccgcggca aagaccggca cgaccaccga gttaccgaac
    18601 tggcgatagg cctgagtgtc cgaaaccgga atgcggaatt tcgcttctcc cggcgcttca
    18661 aaacccatta agcgcgcgca ttcccgaggc gttaaccgac gtggacgatg ttgctgattc
    18721 agcggatcgt caaagtcttt ctcacccgtg gccatatccc agccgcgatc gattaaaatt
    18781 tccgcgccat ctttgtaata acgcgcagac agcgtacggg tgacgctttg cggattgttc
    18841 ggataaacca ttccataacc gaagccgttt ccgcgcgcct gatgtttttt cgcatatcga
    18901 tagaggtact tccacagcac cggcgtcagg atatatttcg cctcgaccat cgggtccaac
    18961 agctgcgcca gcgtcactcg ctgtgcaggg aaacattcgc tgatatcacg cagagtaaaa
    19021 tcggctttca gattaagatc gcgacgaaaa cccaccagca cgatgcgttc acggtgctgc
    19081 ggcagaaaat gtttgccatc gatgattttc ggatcgtccg gcccgttatc ttctgcatca
    19141 gccacgtcat agcccagttc gtccagcgtc tgcatgatga tgcggaacgt tttaccctgg
    19201 tcgtggcttt tcaggttttt gacgttttcg agcacaaaca tcgccggacg acgcgcgtcg
    19261 ataatgcgca ccacatcaaa gaacagcgtg ccctgagtat cgcaggcaaa accgtgcgcc
    19321 cgcccgagcg agttcttttt cgatacgcca gccagcgaaa atggctgaca agggaaaccg
    19381 gccagtaaaa catcgtgttc aggaatgtgt tgacgaatat gttccgccgc agcctcatca
    19441 ctcacgcctt ctttatggct gagggtgatg tcgcggatat cttcattaaa atgatgcgtc
    19501 gccgggtcgc aataatgatt ggctttataa gtgcgtaccg catgtttgtt ccattcgctg
    19561 gtaaacacgc actgtccgcc aatcgattca aaaccgcgac ggatgccgcc aattccggca
    19621 aatagatcga taaagcgaaa cgcataatgc ggatgatgtg ccggtggttt gggtaacaac
    19681 gtttgcagat gggcgaactc tttctcactt aaacggtgcc atgccgagtc attcgccagc
    19741 gcacgtttta aaattgccgc gctccagtga ttctcaccca caccattaag ctgagccacc
    19801 agcgttttaa catcataaat ttgcagcagt ttctccagca ttgcctgtgc ggcattcccg
    19861 gtgctgtatg aatcggttac tgatatattt tcctgcatag atttaaccgg ccatctgaga
    19921 tggtcgacag gttaacataa ttacagccat ttagggccac gcgctcagcc cgcgggtgaa
    19981 aacgcatcta tcaccttgtg atcgacacct tcattctccc ccgccagttc ggcactgagc
    20041 ttcgccataa actcgactaa aaaatgcgcg ttgtgctgcg ccagctgctt gcctcttacg
    20101 gtttgcatga tttgcggtag cttcaacaac ttagtctgga aatgatccag cgcgtagcgt
    20161 ttatcatcaa gcgggcgatg ctgtgcgagc ggatcttcgg catcaaacag cgccacgccc
    20221 aatgcccctg aaacggcaaa cacgcgcgcc aggccaatcg cccccagggc ttccagccga
    20281 tccgcatcct ggacgatttt agcttccgtt gttaaggggg cgatttgcgc gctgaaactg
    20341 tgagcggcaa tggcatgaca aacggcctcg attttctccg ccggaaattg cacgaactct
    20401 tcacggagca ggcgacgcgt ctcttctgcc gccaggattg aagaactctg ccgttgcgga
    20461 tgatttttcg ccaggctcac aatatcgtgg aaataacagg cggttaaaat caccagcata
    20521 tcgacgtcat catctgccgc cagcttctgt gccgtagccc agacgcggcg aaaatgacac
    20581 acgtcatggg ccgcgtcctg gtgctgatga tggtttttca accagttttc gaactgtgcc
    20641 tgccagtgtt gtaagtccat acgcgctccg ttgccaaaga gtcgccacct tagcagtttt
    20701 tattcatagc gtctgctacg tgcgggacga ggtcgtgtgt accaggcaat gctgcctaac
    20761 aatgccccga ccagagccat aaataaaaat ccgaccagtg ccctaaccac tttcccgcgg
    20821 tagagcctaa atcgctttca aatcccgaca cgggggcatc ggggatccgg ctcatcaccc
    20881 agatgtaacg cgccattgcc cagacgaaat agccacacca tgtataaaaa gcccacaaag
    20941 ccagtttgcc gccagggctg cgagagagtc tctcttccat ctttgtcgaa ccgtattcca
    21001 ttttgagata acccggaaaa ttcagactta gtactattac gtgataaaag tcacattttt
    21061 ccacattgga tgagccagtc aagactcact cattttttga acaaatagat aatttttgcg
    21121 caacggcagt gaatacagga aaaatcgttt tgagcgattt tatttatctt tgagatcggc
    21181 tttattaatt cattcaatca atatattagc actgattaca attatacctt tttatt
//
LOCUS       DBFIFI010000063        21116 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.063, whole genome shotgun sequence.
ACCESSION   DBFIFI010000063 DBFIFI010000000
VERSION     DBFIFI010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21116
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.063"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            260..625
                     /gene="mdtJ"
                     /locus_tag="N7B39_002268"
     CDS             260..625
                     /gene="mdtJ"
                     /locus_tag="N7B39_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HGU2422242.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            612..941
                     /gene="mdtI"
                     /locus_tag="N7B39_002269"
     CDS             612..941
                     /gene="mdtI"
                     /locus_tag="N7B39_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HGU2422243.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(980..1801)
                     /gene="ydgD"
                     /locus_tag="N7B39_002270"
     CDS             complement(980..1801)
                     /gene="ydgD"
                     /locus_tag="N7B39_002270"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HGU2422244.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYDIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKAAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(1901..1984)
                     /locus_tag="N7B39_002271"
     CDS             complement(1901..1984)
                     /locus_tag="N7B39_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2422245.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(2077..2385)
                     /gene="asr"
                     /locus_tag="N7B39_002272"
     CDS             complement(2077..2385)
                     /gene="asr"
                     /locus_tag="N7B39_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HGU2422246.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAKKHSHQQPAKPAAQPAA
                     "
     gene            2656..2760
                     /locus_tag="N7B39_002273"
     CDS             2656..2760
                     /locus_tag="N7B39_002273"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HGU2422247.1"
                     /translation="MNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            complement(2809..4062)
                     /gene="ynfM"
                     /locus_tag="N7B39_002274"
     CDS             complement(2809..4062)
                     /gene="ynfM"
                     /locus_tag="N7B39_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2422248.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKSLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            4169..5062
                     /gene="ynfL"
                     /locus_tag="N7B39_002275"
     CDS             4169..5062
                     /gene="ynfL"
                     /locus_tag="N7B39_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU2422249.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            5197..6417
                     /gene="mlc"
                     /locus_tag="N7B39_002276"
                     /gene_synonym="dgsA"
     CDS             5197..6417
                     /gene="mlc"
                     /locus_tag="N7B39_002276"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HGU2422250.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            6542..7237
                     /gene="bioD"
                     /locus_tag="N7B39_002277"
     CDS             6542..7237
                     /gene="bioD"
                     /locus_tag="N7B39_002277"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HGU2422251.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TEKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(7190..8482)
                     /gene="clcB"
                     /locus_tag="N7B39_002278"
     CDS             complement(7190..8482)
                     /gene="clcB"
                     /locus_tag="N7B39_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HGU2422252.1"
                     /translation="MHHLHTYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(8640..9254)
                     /gene="dmsD"
                     /locus_tag="N7B39_002279"
     CDS             complement(8640..9254)
                     /gene="dmsD"
                     /locus_tag="N7B39_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HGU2422253.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(9297..10151)
                     /gene="ynfH"
                     /locus_tag="N7B39_002280"
     CDS             complement(9297..10151)
                     /gene="ynfH"
                     /locus_tag="N7B39_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HGU2422254.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKTDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFVTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLSSIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(10153..10770)
                     /gene="ynfG"
                     /locus_tag="N7B39_002281"
     CDS             complement(10153..10770)
                     /gene="ynfG"
                     /locus_tag="N7B39_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HGU2422255.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(10781..13204)
                     /gene="ynfF"
                     /locus_tag="N7B39_002282"
     CDS             complement(10781..13204)
                     /gene="ynfF"
                     /locus_tag="N7B39_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HGU2422256.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLISENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTNEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            complement(13265..15691)
                     /gene="ynfE"
                     /locus_tag="N7B39_002283"
     CDS             complement(13265..15691)
                     /gene="ynfE"
                     /locus_tag="N7B39_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfE"
                     /protein_id="HGU2422257.1"
                     /translation="MSKNERMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAV
                     QQASEQVVWGACSVNCGSRCALRLHVKDNEVAWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNITRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKSREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDKTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPER
                     STFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINPIDAQKRGIANGDMVRVYNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
     gene            complement(15890..16195)
                     /gene="ynfD"
                     /locus_tag="N7B39_002284"
     CDS             complement(15890..16195)
                     /gene="ynfD"
                     /locus_tag="N7B39_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HGU2422258.1"
                     /translation="MKLSTCYAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            16303..17013
                     /gene="ynfC"
                     /locus_tag="N7B39_002285"
     CDS             16303..17013
                     /gene="ynfC"
                     /locus_tag="N7B39_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HGU2422259.1"
                     /translation="MKYKLLPCLLAILLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVGCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            complement(17016..17576)
                     /gene="speG"
                     /locus_tag="N7B39_002286"
     CDS             complement(17016..17576)
                     /gene="speG"
                     /locus_tag="N7B39_002286"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HGU2422260.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFSVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(17611..17952)
                     /gene="ynfB"
                     /locus_tag="N7B39_002287"
     CDS             complement(17611..17952)
                     /gene="ynfB"
                     /locus_tag="N7B39_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HGU2422261.1"
                     /translation="MKITLSKRIDLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTGNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            18087..18413
                     /gene="ynfA"
                     /locus_tag="N7B39_002288"
     CDS             18087..18413
                     /gene="ynfA"
                     /locus_tag="N7B39_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HGU2422262.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGPLIALCGMLIIV
                     VGWGRT"
     gene            18619..18705
                     /locus_tag="N7B39_002289"
                     /pseudo
     CDS             18619..18705
                     /locus_tag="N7B39_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19180..19367)
                     /locus_tag="N7B39_002290"
                     /pseudo
     CDS             complement(19180..19367)
                     /locus_tag="N7B39_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686441.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
     gene            complement(19402..19653)
                     /locus_tag="N7B39_002291"
     CDS             complement(19402..19653)
                     /locus_tag="N7B39_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HGU2422263.1"
                     /translation="MSEVIMIVSPGKWVSEEQLIALKGIKKGTLKKAREKSFMEGREY
                     KHVAHDGMPWDNSPCFYNLEEIDRWIERQASARPRRHLT"
     gene            complement(19726..>21116)
                     /locus_tag="N7B39_002292"
     CDS             complement(19726..>21116)
                     /locus_tag="N7B39_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HGU2422264.1"
                     /translation="DAVETAIPDNEKTECKVEVEPSVEREGPFYFLFTDKDGEKYGRA
                     NKLSGLDKALAAGATEITKEEYFARKNGTYTGLPQNANTAQNSEQPEPVKVTADEVKK
                     IMQAANISQPDAEELLAVSRGEFVEGISDPNDPKWVKGIQTRDSVNQNQQETEQNDQK
                     AEQNSPNTQQNEPETKQPEPVVQQEPEKICTACGQSGGGNCPDCGAVMGDATYQEIFD
                     GENQPEVQENDPEEMEGTAHQHKENTGGNQHHASDSETGEASDPLIKANGHHNLTSTS
                     RAGIHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTIDLDTAGGVIDRDTM
                     KWWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVHVWGNGANFDNTILRRS
                     YERQGSPCPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNALDDARYQAKYVSA
                     IWQKLIPSQADS"
BASE COUNT         5154 a         5165 c         5301 g         5496 t
ORIGIN      
        1 caattatcat ccgcgatgac gagaagcaac actgcggata attgtaatat tatggacaat
       61 ttgttcagga cgttattttc tacatttacc catcttcgta cctcatccac tatcttgctc
      121 gtaggtgagc agcactggcg caacgcgctt tagtcctttc ttctgcctaa gcttgcccta
      181 tacgcggggc ttgaccactt tactttggtt tcgctgaatt aagcgaaaat taaaataatt
      241 ctcttgcagg agaaggacaa tgtatattta ttggatttta ttaggtctgg ctattgctac
      301 agaaattacc ggtacgctgt ctatgaaatg ggcgagcgtc agtgagggaa atggcggctt
      361 tattttaatg ctggtgatga tttctctgtc gtatatattt ctctctttcg ctgttaaaaa
      421 aatagcctta ggtgtggctt atgcgctgtg ggaaggtatc ggtattttat ttattacctt
      481 gtttagcgtt ttgttattcg acgaaagttt atcgctgatg aaaattgccg ggttaaccac
      541 cctggtcgcc gggatagtgt tgatcaaatc aggtacccgt aaagcacgta aacctgaact
      601 ggaggtgaac catggcgcag tttgaatggg ttcacgccgc ctggctggca ttggcaatcg
      661 tgctggaaat cgttgctaac gtctttttga aattttctga cggctttcgt cgcaaaatat
      721 ttggcttgct ctccctggcg gcggtgctgg ctgcctttag tgcgctttct caggccgtta
      781 aagggatcga cttgtctgtc gcttatgcat tgtggggcgg gtttggtatt gccgccacgt
      841 tagccgcagg ttggatcttg tttggtcaac ggttaaatcg taaaggctgg attggcctgg
      901 tcttgctgtt ggctggaatg atcatggtga aacttgcctg atgaagacgc tgcccgcgct
      961 gtcgggcagc gtttgaacat tatttttgcg acagttgatc cagcttgtcg cgaaaaccgg
     1021 taacagaaat ggcccggtta tcggcgcgcc agcgatcttt cgcggcagga gccgaacttt
     1081 gcaccccaat taattgccag ccgtcatcgg tatgcaacat cagaggcgaa ccgctgtcac
     1141 ccggcaaggt atcgcactga tgtgacatca ccgacgtttg cgcccagcca gtcacttcac
     1201 agttttgatg actgtacaac gtatcgagat gatcttcagg gtatcctgcc tgagtcactt
     1261 tacgacctgc cgcttttaat gcggcggtaa gcgcggcttt atctccctca aataacggca
     1321 acggcgtaat gccagaaggg ggattacgta gcacaatcaa tccgaagtcc cacggcgcgg
     1381 ctgcgggagg tacaatccaa ccatccccat ctgcttttaa ccgctttccc agtgtcggat
     1441 caacgcggcc ttctatgtcg tggatgtcat agcgccaaag acctttattt gacacaaaac
     1501 gcagcgccac tgctttatcg gctttaccct ttggtggcgt caataaacag tgtcctgccg
     1561 ttaatgccag attgggtgca atcagcgtcg ccgtacataa attgccgctg gccgtttcca
     1621 gttgcccaac cgcatcccac ggtgattggg tcgtgtcatt cactggcaca cgatcatcat
     1681 gaccaaaaaa cagggtgctg acctcatcgt ttgccgattt ggcaacgtct ggtttatctg
     1741 caaacacaaa agcagacgtc aaactaattg cacccaacac tacagcaatg gttgtacgca
     1801 tatcacactc tggtgggtaa ttatgattat taaaagcaaa ccctcataaa tactatagac
     1861 gggacgggat gaaagtggga gtaaaatcag ataactacga tcaggaaaga taaaaacggg
     1921 cggcaatcag tgcgcataaa ataaggatga taagaatgaa ctcaaagcga taacggccca
     1981 ccatactacc ctccgctaaa ggcggtgcta aacgttaccc gcttagcacc gtaattttca
     2041 ggcgcgaggg ggcgcgccag cattactgtt gaaaacttac gctgcgggtt gtgcagcagg
     2101 ttttgccggt tgctgatggc tgtgtttctt ggcgtgtttc ttcgctgcct gcgctttttg
     2161 ttctggggct ttctgttcag ctttcgtatt tttatgatgc tttttagccg cctgcgcttt
     2221 ctgggcaggt gctgctttat gctgtttttt atgatgtgta gttttcgccg gcgctgcttt
     2281 ggtggtcgtc gcagtcggag caggtgtggt cgcagtctct gcagcaaagg cggcagaaga
     2341 cagacccata gcagcggcaa caaccagagc taatactttt ttcattgtca taccctcaat
     2401 ttgttttttc atttaacccc actgcggggc cgttgaaaga actatatccc tgggaatggc
     2461 agacttccgt gagtggttgg tttcagcgtg taacgatatg tacaaacgct gaataaatta
     2521 cagcgttgat aataacgtat tgtgacttaa ggaaaattta gctaccaata atagtagtct
     2581 tgatcgggtt aactatttta ctacttacaa agcggtagaa taactgcgca tcagtaataa
     2641 atgacacaca gcaaaatgaa tccgtttatt tgggtactta taatcctgat gacgctagac
     2701 gcgctgcggg aattggctgg cgcttcgtct attttaggat ggctattaac gttggtttga
     2761 gcaggcaata agtccggacg ggtatttacc gcagtccgga cttatttttc aggcgtgcag
     2821 acgacgatgc aaacgcgtcc cgaccagcag agcaatgacc agcatcagag caataaatgc
     2881 gccgacgccg ttccagccat agttatgcca gaaaacacca cccagcgtcc cggcaatact
     2941 cgaccccaga tagtaactga acagatacag cgaggaggcc tggcctttag cgcgttttgc
     3001 gcgggggccg atccagctgc tggcaactga gtgggctgcg aagaatcctg ctgagaagag
     3061 taacattccg gcaaagatca gccacagcga gctgaataag gtcatcagta aaccaaacag
     3121 cataaccccc gtcgaaaaca acatcactgg accacgccca tagcgggtgg tcatggttcc
     3181 ggctttgggt gagctccatg taccggtcaa ataagccagc gataataagc caaccacggc
     3241 ctgactgaca tgccaggggg agagcatcaa ccgatagccg atgtaattaa acagcgtgac
     3301 gaacgacccc atcagcaaaa agccttctgc gaacaataac ggtaatcccc ggtcacgcca
     3361 gtgcagacga aagttgataa acaacgactt aggtcgcagc gaagtcgggc gaaaatggcg
     3421 tgattccggg aggattttcc agaacatcaa cgccgaggct agcgcgaaac aaccgatagc
     3481 cgccagagca attcgccagt tgaaaaagtc cgtgaagaca ccactaatta agcgtccgct
     3541 catgccgcca attgagttgc cgctgatata caaccccatc gaaaaggcca cgaaactggg
     3601 gtggatttcc tcgctaagat aagtcatgcc aacagctgcc acgccactta acgaaagccc
     3661 aatcaaggcg cgcataatca aaatgccgtg ccagctggtc atcattgtcg aaagtaacgt
     3721 acaaatggag gccaatagta gcgccgttac catcactggt ttgcgaccaa tggcatcgga
     3781 tagggggcca gtaaacagca aaccaatagc caacatcgcc gtggaaatgg acagtgaaat
     3841 actactgttc gcgggcgtta agccaaactc ctgcgaaagc accggaagga taggttgcac
     3901 acaatagaga agtgcaaatg ttgccagtcc ggcagagaac agcgccaggg tgacgcgcat
     3961 aaattgcggc gtaccgcgtt taataaattg atttggctga gaaatgcttt gcttgtcagt
     4021 gtcgcttgcc ggagcgccat caacagttgt agtacggctc acttgaaatc cttgctaaat
     4081 atgcctgtgg atcaggctta tacataggtt aggaaaatcg aattgttctg tctaatatat
     4141 taataatctc aaataagatg ttttaaatat gaatattgaa cttcgtcatc tgcgttactt
     4201 tgttgctgtt gcggaagagc tgcatttcgg gcgcgccgct gcccgcctga atatttcgca
     4261 accgccgcta agtcagcaga ttcaggcgct ggagcaacaa attggtgccc gactgctggc
     4321 acgaaccaat cgcagtgtat tgctgacggc agcaggaaaa cagtttcttg cagatagtcg
     4381 gcaaatcctg tctatggtgg atgacgctgc cgctcgcgct gaaagactgc atcagggtga
     4441 agcgggggag ttgcgcattg gttttacttc gtcggctcct tttattcggg cggtgtccga
     4501 tacgttatcg ctgtttcgcc gagattatcc cgatgtccat ttacaaaccc gcgaaatgaa
     4561 cactcgcgag caaatcgctc cgctcattga aggaacgctg gatatgggat tgctgcgtaa
     4621 cacagcgtta ccggagacgc ttgaacacgc agtcatcgtc catgaaccgc ttatggcgat
     4681 gatcccgcac gatcatcccc tggcaaataa cccgaatgta acgctggctg aactggcgaa
     4741 agaacccttt gtcttttttg atccgcacgt cgggacaggg ctgtatgacg atattctcgg
     4801 gctgatgcga cgttaccatt tgacgcccgt catcactcag gaggtgggcg aggcaatgac
     4861 catcatcggt ctggtttccg ccggtctggg tgtttcaatt ttgcctgcgt catttaaacg
     4921 tgttcagctc aacgaaatgc gctgggtgcc gattgctgaa gaggatgcgg tttctgaaat
     4981 gtggttggtc tggccgaaac atcatgaaca aagtccagct gcgcgtaact ttcgtattca
     5041 tctgctgaat gctctcaggt gagggaaatt tcagcgaaaa agcccgaaaa atgtgctgtt
     5101 aatcacatgc ctaagtaaaa atttgacgac acgtattgaa gtgcttcacc atagcctaca
     5161 gattatttcg gagcgcgaaa atatagggag tatgcggtgg ttgctgaaaa ccagcctggg
     5221 cacattgatc aaataaagca gaccaacgcg ggcgcggttt atcgcctgat tgatcagctt
     5281 ggtccagtct cgcgtatcga tctttcccgt ctggcgcagc tggctcctgc cagtatcact
     5341 aaaattgtcc gtgagatgct cgaagcacac ctggtgcaag agctggaaat caaagaagcg
     5401 gggaaccgtg gccgtccggc ggtggggctg gtggttgaaa ctgaagcctg gcactatctt
     5461 tctctgcgca ttagtcgcgg ggagattttc cttgctctgc gcgatctgag cagcaaactg
     5521 gtggtggaag agtcgcagga actggcgtta aaagatgact cgccattgct ggatcgtatc
     5581 atttcccata tcgatcagtt ttttatccgc caccagaaaa aacttgagcg tctaacttcg
     5641 attgccataa ccctgccggg aattattgat acggaaaatg gcattgtaca tcgcatgccg
     5701 ttctacgagg atgtaaaaga gatgccgctc ggcgaggctc tggagcagca taccggcgtt
     5761 ccggtttata ttcagcatga tatcagcgca tggacgatgg cagaggcctt gtttggtgcc
     5821 tcacgcgggg cgcgcgatgt gattcaggtg gttatcgatc acaacgtggg ggcgggcgtc
     5881 attaccgatg gtcatctgct acacgcaggc agcagtagcc tcgtggaaat aggtcacaca
     5941 caggtagacc catatggtaa acgctgttat tgcgggaacc acggctgcct cgaaaccatc
     6001 gccagtgtgg acagtattct tgagctggca cagctgcgtc tcaatcaatc catgagctcg
     6061 atgttacatg ggcaaccgtt aaccgtggac tcattgtgtc aggcggcatt gcgcggcgat
     6121 ctgctggcaa aagacatcat taccggggtg ggcgcgcatg tagggcgcat tcttgccatc
     6181 atggtgaatt tatttaaccc acaaaaaata ctgattggct caccgttaag taaagcggca
     6241 gatatcctct tcccggtcat ctcagatagc atccgtcagc aggcccttcc tgcgtatagt
     6301 cagcacatta gcgttgagag tactcagttt tctaaccagg gcacgatggc aggggctgcg
     6361 ctggtaaaag acgcgatgta taacggttct ttgttgattc gtctgttgca gggttaacat
     6421 tttttaactg ttctaccaaa atttgcgcta tctcaatttg ggccaggaaa gcataactta
     6481 gactttcaag gttaattatt ttcctggttt atatttgtga agcataacgg tggagttagt
     6541 gatgctgaag cgtttcttta ttaccggtac agacacttct gtagggaaaa cggtggtttc
     6601 ccgcgcattg ctacaagcgt tagcctccca gggaaaaacg gttgcgggat ataaacccgt
     6661 agcgaagggg agcaaagaga cacccgaagg gctgcgtaat aaagatgccc tggtgttgca
     6721 gagtgtttca accatcgaac tgccttatga agcagttaat cctatcgcgt taagcgaaga
     6781 agaaagtagc gtggcgcaca gttgcccaat caattacacc ctcatttcaa acggcctggc
     6841 aaacctgacc gaaaaagtcg atcatgtcgt ggtagaaggg actggcggct ggcgtagtct
     6901 gatgaatgat ttgcgtccac tctctgaatg ggtggtgcag gaacaactgc cggtgttgat
     6961 ggttgtcggt attcaggaag gttgcattaa ccatgcactg ctaacagctc aggcgattgc
     7021 caacgacggg ctgccgctca ttggctgggt ggctaaccga atcaacccag gactggcgca
     7081 ttatgcggaa atcattgatg tgctcggtaa aaaacttccg gcaccgctca ttggtgaact
     7141 gccttatctg ccgcgcgccg aacagcgtga actggggcaa tacatccgct tagctatgct
     7201 gcgcagtgtg ctggcggtag atagagtcac ggtgtaacgt ccgcgagatt accgacgcaa
     7261 ttacgcaggc aatcaataaa ccggggagta gctgatactc cccggtcatt tcacatatca
     7321 tcaacgtcga cataatcggc gcgtgcgtgg ttgccgccaa cagtgtcgcc atcccggtca
     7381 atccgagtaa aagcgtaatt tcttcgccat cagggaacca taatcccaga ctacgaccat
     7441 acaacatgcc aatggcaaga ccgataaata gcgtcggtgt aaagacacca ccgggtgcgc
     7501 cggaaccgct actcgccagc acggcacaca gtttacaaag gaagatcccg gcaatgatca
     7561 ttaacagtgg cggggcggtt aaaaaggatt gtacggtgct atagccgttg ccccacactg
     7621 caggtgtgaa cagggaaagc agacccacga tcaacccgcc tagtgccagt tgccagggcg
     7681 gcgcaagttt gagactcaca aatccacgat gacaggcgtt cattaacgtt aacaacagtg
     7741 gtccgcacag acctgccagc acacctgtac tgataatcag cgcatagtca cgagcctgaa
     7801 ccgtcactga gagttgtacg ctgtagagta acgcgtcgct atgattaatc agattgctaa
     7861 ccagcaatgc cacgacggcg gaaataatca ccgggccgag agaggccaac atcatagtgc
     7921 caaacagcac ttcggctata aataaactgc cagcaagcgg ggcacgatag gccgcagcca
     7981 ttcccgccgc ggccccacag gcgatccata atttccactc ctggcgtggc gtaaaacgtt
     8041 gggcaaaaca ggaggcggca agggcagcta aaagaatcat cgcaccttcg cgaccaattg
     8101 cactgccgct ggttactacc agcagagagg caagcgattt aaccaggctt gctgcgtaat
     8161 caaactgacc atcggtttgc aacgcttcca tgtaatcggt cggcgcatga gggcgttgct
     8221 gggtaaattt ctgccagccc atcaacaaca aacccgccgc cagtccgccg agcgccggag
     8281 ttagcagccg tcgccagggg gaaaggtttg tcgctgcatt gaccagactg ccggagtcat
     8341 tattgaggaa caaccactcc agtagcagca tcgcatgacg aaacccggca acggcaaagg
     8401 ccgcgagaat accgacgact gttgcgataa gcagacggcg gaacatagtg cgcagatccg
     8461 gataggtgtg tagatgatgc atgagcagaa atgggcaatg aaacacgggc gactattttg
     8521 cggcaaaaga ggccagctgc aaaagcagtg cgtgctttaa gagacaaaaa acgtgacact
     8581 gtaacgtatt gttgccgaat cgcgcgtaat cgtcaccatc cggcaatatt acggtgatcc
     8641 tatcgaaaga gtggtttaac cgcgacagga attaacagtt gtgattgcca ttgcgccagc
     8701 gttaagcgag ccagttcacc cagtgcacgg tagaaagggt gttctgcttt ttcgataaaa
     8761 acgtcaagaa aacgtgttga ccagggaaaa aggtgccatg ccagcaattc ttcgcattct
     8821 gtctggcgac cattctccgc caaccacgca gccatcaaca gcagtgaacc aaaatgatct
     8881 tccggttcgt tttgcttcat ttcaaactga atgcctttct cgcgcatcca ctgacgaagt
     8941 gccaatgttg aatcgccaaa cagcacagat tcgcgatcca gccagaccga cccccacggc
     9001 ggagacggca gtgcccacgg gccgacaaac aaacgctgcc aggcctgggc gtgagtctct
     9061 tcacactgtg tctgaaaagc cgtcaccagc ggcgctaatg acgcttctgg tagaggccac
     9121 tgagtttccc agccgtcact ggtgagtacc gcaaccagag gtgcggcttc agcactctct
     9181 ggagcgtaat aaaacagcgc gcccaacacg cgcgccgcga cagaaaaatt atcttgctgt
     9241 gaaaaatggg tcattcctaa catcctgaaa tgtgcgggtt gccccgcacc aatttgttaa
     9301 cctgcaatag ccattcctac cgtcatgtgc aggccataaa agagcacacg cccgatcatc
     9361 tcaccgccaa gaatgaggat taaccccaga ataagtccgg caacgtgagg ttcacggcga
     9421 cgtatgagcg gacaaagcca gcatcctaga cctgcgcata acaataccac acgccagacc
     9481 tgtaaggagg cgtaatctgg taccagtgcg ctggcttgtt gcacggatga gtgaatagac
     9541 gacagagaca aaccttgcag cacaatcacc ccggcgcaag cgatcagcgc cagaacgcta
     9601 atgatggcaa atggtgtggt attaaatgta acgcgggctg cccgcagaat tgcggcagca
     9661 agtattggac cactcaacaa taccgtcacg aagaaagcca gcgtagtgta accgttatgc
     9721 caggttggca cagtgtcgat ttgatacacg caggtcatca tccagacgaa aatgacgccg
     9781 agcgccatgc tgacaagtaa ccagagtttc cccaacgctt gcggcatttt accgatgacc
     9841 gccaccagcc accatagtcc gccaacggca aaaaatatgg aaccggcagc aatctcgtta
     9901 ctcagaccag aagctccgat tcgattcagc gagttaaacg cccgcagtgg cgatcccagg
     9961 tgcatgacag aggcaataaa cccgatgccc atcaacagcc agaggaaaaa catgccacga
    10021 acaatgtgct gacggtctgc gtcagttttg gcggcgaacc agccaattcc gctaacaatc
    10081 agcgcgccta ctacgcattg acccaatacg gtaaagataa ccagtggcca ttcatgccat
    10141 ccatttccca ttttacactt cctccggatt agccagataa ccagtggtat cgccggtcgg
    10201 gcggctgttg gcgttaggtt tgataacgat attgggtttt gtgaaatgcg cgcgcggcag
    10261 tggcgcgacg gcagcaagag tgccgtgctt ctggcgcagc tcttcaattg gaccgaactc
    10321 aagtgcacgc agcgggcagg attcgacaca tatgggttgt ttcccctcgg cgacgcgcga
    10381 ataacaacca tcgcacttcg tcatgtgccc tttttcagca ttgtactgtg gcgcgccgta
    10441 cggacaagcc atgtggcagt agcggcagcc gatacaaaca tcttcatcaa ccaccacaaa
    10501 gccatcttca cgcttatgca ttgcgccgct ggggcagact tttgtacatg cggggtcatc
    10561 gcaatggtta caggagatgg agagataata agcgaacacg ttctggtgcc agacgccatt
    10621 atcttcctgc cagtcgccgc cagcatattc ataaatacgg cggaaactga cttccgggcc
    10681 aagatcttta aaatctttgc acgccagttc gcaggtttta cagccagtac agcggctgga
    10741 atcgataaaa aatccatatt gtgtggtcat gggctactcc ttaaaccttt tcgatctgga
    10801 caagattgct gtgcgacggg tttccctttg ccagcggtga agggcggtga gaggtcagaa
    10861 tattgatact gccgccgtga tcgacccggt caccaaacat atccgcttta agccacgcac
    10921 cttgcccgat ggcggtaacg ccaggcagaa tacgcggagt cacttttgcg gcaatcagca
    10981 tttctccatt attgttaaat acccgcacgg tatcgccatg acggataccg cgagcctgag
    11041 catcaatggg gttgatccac acctcttgtg ggcaggcctg ctgtaacaca tcaatattgc
    11101 cgtagctgga gtgggtacgc gctttgtagt ggaagcccgt taactgcagt ggataggttt
    11161 tccgcagggg atcgtcccag ccatcaaaac ctggggtata cgcaggaagg ggatgaataa
    11221 tttcatcttt tttcaattcc caggtatctg caatcttcgc cagtcgttca gaataaattt
    11281 cgattttccc cgaaggtgtt ttcaacgggt ttgcctgtgg atcttcacgg aatgcgcgga
    11341 aagcgacgta gtgttcttcc gggcattttt tcttaaagat cccggtcgtt ttcatctcct
    11401 cgtagtcggg catctcaggg ttacgttcct tcgttttcgc atggagatat ttgatccatt
    11461 catgctgact gcgaccttca gtaaaggttt gataaacgtc cggtcctaag cgtttggcga
    11521 cttcactcag catccagtag atgggtttgc gttcaaattt tgctgaggtt gcgggttggg
    11581 cgaggatcac atagcccata ttccctgcag attcatgaga gataaggtct tcttgctctg
    11641 ttggcatcag gtcgggcaac aggatatcgc aatacttagc cgaggccgtc atgaagtggt
    11701 caatgccaac aatcatctcg cacttgctgt catcctgaag cacctcattg gtgtgattga
    11761 tgtcgccatg ttgattgatc aatgtgttac tggcgtagca ccataaaaac ttgatgggga
    11821 catccagttt ttcttttcca cgaacaccat cacgggtcgc ggtcatttcc gtaccatggt
    11881 cgatggcatc tgtccatgta aagacggaaa tctgcgtttt aacaggattc tcgagcatcg
    11941 ggaaccattc tacccccaga tcccagctac cttcgcgtac acctgagttg ccgccgttta
    12001 tgccgacgtt accggtgaga acggaaagca tggcaatagc gcgggatgtt tgctcgccgt
    12061 tggaatgtcg ttgtggcccc caaccctgac aaatataagc aggttttgct gaaccgatct
    12121 ctcgtgccaa ctggataatt ttttctgccg ggatgctggt gatttttgct gcccattccg
    12181 gcgttttagc tatgccgtca ggcccttcgc ccagaatata ggctttataa tgcgcgttac
    12241 gtggtgcgtt ggcgggcagc gttttttcat cgtaaccaac acaatatttg tcgagaaatg
    12301 gctgatcgac catgttttca ctaatcagta cccaggcaat cgcacaggcc agtgcgccat
    12361 cggtgccagg gcgaatgggc agccattcat cttcacgccc ggcagctgtg tcgttataac
    12421 gtggatcgat gacgatcatg cgtgcgtttg aacgttcgcg ggcttgctcg acgtagtaag
    12481 tgacaccacc accgctcatc cgcgtttctg ccgggttatt tccgaacata acgaccagtt
    12541 tcgtattggc gatatcatcc gggctgttgc catcattggc accgaacata taactcattg
    12601 cggcactgat ctgtgcggta ctgtagctgc catagcgact gagaaaacca ccgcaagagt
    12661 tcatcagacg gtacgggacg tttgagttgg tgatgtttcc gccatctacg cctgttccgt
    12721 acaggacatg tacagcctca ttgccgtaat ctttcaggat ccgccgaaga ttatcactga
    12781 tggtatccag ggcttcgtcc caacttatcc gttcaaattt accttcaccg cgcttgccga
    12841 cgcgcttcat gggatatttc aacctatcag gatgattcat ccgtcggcgg atagagcgcc
    12901 cgcgtaaaca cgcacgaacc tgatgattac cgtagacgtc gtcacctgtc gtatcagact
    12961 ccacccagta cacggtgtca tctttcacat gcaaacgtaa cagacagcgg ctcccgcagt
    13021 taacggtgca ggaactccag accgctttct cttctaccgg tgcctctgcc gctcggacca
    13081 tttgggaaaa tggcagagtg aaagcactgc ttgccagcgc aagactgcca agtgcggagg
    13141 ttttcatcag acttctacgg ctgatttcag ccttcatgag cgcctctgtg gtatggattt
    13201 tcatcattac tcacttattg cttttcaaac aaaatgtcat gccagaattt atggttgtag
    13261 tgggttatat tttttcgatc tcgaccagat tcgtgtgctg cgggtttccc ttcgccagtg
    13321 gtgaagggcg cagagtggtt agcgtattca cacatccgcc atggtcgatt ttatcgccag
    13381 acatattggc ctcgtgccag gctccctggc ccatagcgct aaccccaggg agaatgcgtg
    13441 gtgtcacttt ggctggtagc cgaacttcgc cacgatggtt atacacccgc accatatcgc
    13501 cgttggcaat cccacgtttc tgtgcgtcta tagggttgat ccacacctcc tgacggcagg
    13561 cagccttcag gacatcaatg ttgccgtagg tcgagtgagt acgggatttg taatggaaac
    13621 caaacagttg cagtggaaag gtactacgtt caggggagtc ccagccttca aaggttgagg
    13681 cataaactgg cagtgggctt atcacttcat ctttttccag ttcccaggta cgggcaattt
    13741 ccgccagctt gctggaataa atttcaatct taccggaagg tgttttaagt ggatttgcct
    13801 cggggtcgtc acgaaatgct ttgtaggcga caaaatgacc attgggatct ttacgcttat
    13861 agatacccat ttttttcagt tcgtcgtaag acggtaacgc cggatctttg gcaagcattt
    13921 tggcgtacag atgttgtaac cattgttcct gcgtgcgacc ttcagtgaac ttttgataga
    13981 cgtcaggtcc aagacgtttc gcgacttcac tcaggatcca gtaaatcggt ttgcgttcga
    14041 atttttcgct ggtgacaggc tggaggaaaa tgagatatcc catgttaccg gcgtagtcgt
    14101 taggaataat atcttcctgc tcaacagtca tcaggtctgg cagcagaatg tcggcatatt
    14161 ttgccgatga ggtcataaag ttttcgatga ccacaatcat ttcgcatttc gattcgtcct
    14221 gcagaatttc atgcgttttg ttgatgtcag aatgctgatt aaccagggta tttcccgcgt
    14281 agttccagat gaacttaatg ggcacatcca gtttatcttt gccccggaca ccgtcgcgga
    14341 ttgccgtcat ttgtgggcca tgatcgatag catctgtcca gctgaagcag gaaattgacg
    14401 ttttgaccgg attatccagc accggcaggc gctctatggt aatggtatag gtcgattcac
    14461 gcgcgccact atttccgccg ctgatgccga cattgcccgt cagaataggc aacatagcaa
    14521 tagcgcgtgc ggtcagctcg ccgtttgcct ggcgttgcgg cccccagccc tggcagatat
    14581 aagcgggttt tgctgtgcca atttcacgcg ccagtttgat gatacggtcc accgggatac
    14641 cggtaatttg cgaagcccac tgcggcgttt tcgctgtttt atcgtcacct tcaccaagaa
    14701 tataggcttt atagtgacca tttttgggtg catctgcggg taaggttttt tcgtcatagc
    14761 cgacgcagta tttatcgaga aaaggttgat caacgagatt ttcgttaatc aatacccagg
    14821 caatacccgc aaccagcgcg gcatcggtgc ccgggcgaat aggaagccat tcatcttcac
    14881 gaccagcagc cgtatcggta tatcgcggat cgataacaat catttttgcg ttcgattttt
    14941 cgcgggattt ttcaagaaga taagtgatgc caccgccgct catgcgggtt tctgccgggt
    15001 tgttaccaaa catcacgacc agcttgctgt tttcaatatc cgtggtgctg ttgccatcat
    15061 tactgccgta ggtgtagggc atggcacagg aaatttgcgc agtgctgtag gagccatact
    15121 ggttgagtga accgccgtag cagttcatca ggcgtttgac cgccgaggct gatggcgaag
    15181 agcgggttat attgccgcca acgatccccg aagagtactg aatatataca gcctcattgc
    15241 catattgttc gacggttttc ttcaggctac tggcgatagt atccagggct tcatcccagc
    15301 taatccgttc gaatttgcct tcaccgcgtt tacccacgcg tttcattggg taattcaagc
    15361 gatcgggatg attaatacgc cggcggatgg agcgaccgcg caaacaggcg cgtacctgat
    15421 ggttgccata ctcatcgctg ccggtgttgt cagtttccac ccaggccact tcattatctt
    15481 taacatgtag acgaagtgca cagcggctac cacagttgac ggaacaggca ccccagacca
    15541 cttgttcgct ggcctgttgt actgctgccg ctgcactgcg cagggtgaac ggcaaagaaa
    15601 aaccgcctgc agccagcgcc agagaaccta tcgcggtaga tttaacgagt gttctgcggc
    15661 tgatgcccac cattcgttca tttttggaca taactcactc cctgttcttt atcgttatat
    15721 aaaagtttat atattgaata tttagcgcgc taacaataga gggagtctac ccattttggg
    15781 ttaataatta ttaatccata tcaatagaag ggtatgagta atatggtggg attatgttgt
    15841 atgtccaaat cgccggatgt gtcgtatccg gcgtgtagtc gataatgtat tactgcggtt
    15901 cggcaggcgc gccatcctgg gtagactgcg cgggagcaga gacgttaccg ctggtggtgc
    15961 gggtgtagag aattttatgc gtatcattag cgcaatgccc gacgacctgg gaatcaggct
    16021 gatcaacttg gtcattgggt acaatactta acgtgaagct gctttcgggt acgccattat
    16081 tgataatgcg ctgtgatata tcgctttgta tgcgctcaca ggatcccggc gcggcgagta
    16141 ccgcgggtga ggcgagggcg agcagaagcg cggcataaca ggttgagagt ttcatcataa
    16201 gctcctaacg cgaagataac ttctttaagc atagcatttc acgtgtaaag tactgtattt
    16261 gctactatga ttgagaatca tctctactct ctggtgactg atgtgaaata caaattactg
    16321 ccatgcttac tcgcgatatt actcacagga tgtgaccgca cagaggtaac actttcattt
    16381 actcctgaga tggccagttt ctctaatgaa ttcgattttg atccgctgcg tggtccggta
    16441 aaagatttca ctcagacatt aatggatgag caaggtgaag tgacgaaacg tgtttcgggg
    16501 actttgtcgg aagaaggctg ttttgattca ctcgaattac tggatctgga aaataatacc
    16561 gtggtcgctc tggtactgga cgccaattat taccgtgatg ccgagacgct ggagaagaga
    16621 gtacgtttac agggaaaatg ccagctagca gaattacctt ctgccggggt gagttgggaa
    16681 accgatgata atggcttcgt gattaaagcc agcagcaaac aaatgcagat ggaatatcgc
    16741 tatgatgatc agggttatcc actgggtaaa accacgaaaa gtaacgacaa aacattatct
    16801 gtcagcgcca cgccatcaac ggacccgatc aaaaaattag attacacagc ggttacttta
    16861 ctgaataatc aacgagttgg taatgtaaaa cagagctgtg aatatgacag tcacgccaat
    16921 ccggtgggct gtcagctgat cattgttgat gaaggagtaa aacccgccgt cgaacgggtt
    16981 tacaccatca aaaatacgat cgattattat taatgctatt gtgcggtcgg cttcaggaga
    17041 gtctgacccg gtgttttgtg ctctgccaga tactgatgct ggaatataca catgcgaatg
    17101 gcattacgat attgaccatt aataaagaac tcgtgcatca attcaccttc aaccgaaaag
    17161 ccaagcttgc ggtaaatgtg aatcgctttt tcattctctt tatcaacgat cagatacagc
    17221 ttatagagat tgagaacggt gaagccatag tccattgcta atttggcggc acgggttgcc
    17281 agacctttcc cctgatactc cggggagata attatctgaa attctgcgcg gcgatgtaca
    17341 tggttaattt ccaccaactc caccagaccg gctttttcgc cgtcacattc caccacaaag
    17401 cgtcgttcgc tctgatcgtg aatatgctta tcatacagat cagagagttc aacaaaggct
    17461 tcgtagggtt cctcaaacca gtaacgcatc acactggcgt tattgtcgag ttgatgtaca
    17521 tagcgtaaat cttcacgctc cagcgggcgt agcttaacac tgtgggcgct tggcataacg
    17581 tgtccttaca ttccttaaat caataacagg ttagggggta ataacgcggc cagttcgacg
    17641 gtccaggcag cgcaaagtat ttggctccca gtaggcattg atgttggcgc tttgctcgca
    17701 tttatcgcgg ttatcaaaag cggcgtcggc tttatcccac tctttttcag tgcgtttatt
    17761 cactttctgg cgcagattgc ccgtgtcatt ccattgctct ttttccatag cggcgtgctg
    17821 gcggctttgt gcactgtcgc cagactcaat caccagtttg ttagtttcgg catgaacagt
    17881 tgtgctcaat gccagtgcgc aaggcagcag aaaagcgagc aggtcgattc gtttgctgag
    17941 agtgattttc ataattcatt ccctgtatga atgattaaag atgattctac accatccact
    18001 gcgaacgcaa aacgtaccag gagggtgttt atattgatga tattatgtcg ccctataact
    18061 atacatgatg tcaataagtg acaaagatga ttaaaacaac gttactattt tttgctactg
    18121 cgctgtgtga aattattgga tgctttctgc cctggttgtg gttaaaacga aacgccagta
    18181 tctggctgtt gcttccggcg gggatttcac tggcgctgtt tgtctggttg ttaacgttgc
    18241 atcctgcggc gagtgggcgt gtttacgcgg cttatggtgg cgtttatgtc tgcacggcgt
    18301 tgatgtggct gcgcgttgtg gatggcgtga aactgactct ttatgactgg acggggccgt
    18361 tgattgcgct ttgcggcatg ttgatcattg ttgtgggctg ggggcgcacg taggaacata
    18421 aatccatttt atcaataaga tacgaggaag tgtcagctga caaaaggtat tctatttcat
    18481 cttttgtcaa ccattcacag cgcaaatata cgcctttttt tgtgatcact ccggcttttt
    18541 tcgatcttca tacttatatg gtagtagctc agttgcgtag atttcatgca tcacgacaag
    18601 cgatgcaagg aatcgaacat gaagatcgta aaggctgaag tttttgttac ctgtccgggg
    18661 cgtaatttcg tcacattaaa aatcaccact gaggacggta ttacaccata aatttttggc
    18721 ttgctgattt ctgcacgcat cgataagacg gatgaattcc tcccgcgaaa gaggatccgg
    18781 aatggttctt gattccttta atggcgagat ccccttaaac gggttatctg ccaggtaacc
    18841 gttatcaaca ccaaactgga acacggcgtt aagatttgtc atgtaattat ttacagttac
    18901 agccgatctc cctggttgtg taacaatata gttacttttg gggatctggt atccagtcag
    18961 tagctcttta cgaacctcca gtaatttttc tttattaatc gatgaggcaa gatttttttc
    19021 accgattatg ctcaggatat ttttgatgac ggcacggtat gtgttgagtg atgtttttgc
    19081 gacttcagtt tctttcagtg ccagaaattt ttcagccagt tcttttatgg ttaaatcttg
    19141 tcgggcctca ccaaattttt ccagattgcg tgaggaggga aactgttttg catagtcgaa
    19201 aacaccagtt tttattgcgt aacaaacaga ggcgcgtagc tcacctgcaa cgcgcctgtt
    19261 ttttgctgtg tcaggaaccc caggttttcc ctgactctta cgcctttata aacaaaccag
    19321 atacgtaatt tccctccatg gttttccacg cctgtcggat atttcatttc aacttctctc
    19381 attagttagt gtggctttta gtcaagtaag atgacgtctt ggtcttgctg atgcctggcg
    19441 ctcaatccag cgatcaattt cttccaggtt gtaaaagcat ggactgttat cccatggcat
    19501 accgtcatga gcgacatgct tatattccct tccttccata aacgattttt cccgggcctt
    19561 ttttaacgta ccttttttta ttcctttcag cgcaattaac tgctcttcgg atacccattt
    19621 gccgggagag acaatcatga ttacttcgct catcgatttc tttatctctt acatcagacg
    19681 agcgccggtt gcagaatacc agtcacaacc ggcgacagtt gaacattaag aatcagcctg
    19741 actcgggatc agtttttgcc agatagctga aacgtatttt gcctggtaac gggcgtcatc
    19801 aagtgcatta tggcgctcac cttcgaatgg aatagccgtt ctggcatcga agtctatggc
    19861 tttccccagc tcaacgattg tgcgtacatc gcgatcgttg tagtaacgcc acgggcaggg
    19921 gctcccctgc cgttcgtatg aacggcgcaa aatcgtgttg tcgaagttgg ctccatttcc
    19981 ccagacatga acaaaaaatt caccggagtt ttcgtcgata aattcccgca attgtaacag
    20041 tgcatcatct aacgggattt catcggtcat aatggcagat tgcgcttcgc gtgattgttt
    20101 aagccaccat ttcatggtgt cccgatcaat gactccgcca gcagtatcca gatcgatagt
    20161 cttactaaat tccggtccca tatctccggt ttgcggatcg aaaaatattg cacctattga
    20221 gataatcggg gcatcgggat tttttcccat ggtttcaaga tcgatcatca gatgaatccc
    20281 cgctctgctg gtggatgtga gattatgatg accgttcgcc ttaattaagg gatctgacgc
    20341 ctcgccagtt tcactatcgc tggcatgatg ctgattgccg ccagtgttct ccttgtgctg
    20401 atgtgcagta ccttccattt cctccggatc attttcctga acttcaggct gattctctcc
    20461 atcgaatatt tcctggtatg ttgcgtcacc catcaccgca ccacaatcag ggcagttgcc
    20521 gccaccgctc tgaccgcagg cggtgcagat cttttccggt tcctgttgca ctactggttc
    20581 aggttgtttc gtttctggct cgttttgttg cgtatttggg ctgttttgtt ccgctttctg
    20641 gtcgttctgt tccgtttctt gctggttctg gttcacagaa tcgcgggtct ggatcccctt
    20701 aacccatttc ggatcattcg ggtcgctaat cccttcaaca aattcaccac gtgatactgc
    20761 aagcagttcc tcggcgtcag gctggctgat attggctgcc tgcataattt tctttacttc
    20821 gtcagcggta acttttaccg gttctggttg ttcagaattt tgtgcggtat ttgcattttg
    20881 cggtaagcct gtgtatgtgc cattttttcg ggcaaaatat tcttcttttg tgatttcagt
    20941 agccccggca gccagcgcct tatccagacc agaaagtttg tttgcgcgac cgtatttttc
    21001 gccatccttg tcggtgaaga ggaagtagaa cggcccctca cgttctacag atggttcgac
    21061 ttccactttg cattcggttt tttcgttgtc tggaattgcc gtttccactg catcag
//
LOCUS       DBFIFI010000064        20839 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.064, whole genome shotgun sequence.
ACCESSION   DBFIFI010000064 DBFIFI010000000
VERSION     DBFIFI010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20839
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.064"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            244..750
                     /locus_tag="N7B39_002293"
     CDS             244..750
                     /locus_tag="N7B39_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HGU2422265.1"
                     /translation="MKSVLLVGLLALTSPVIAQDCFELAGRDYKIDPDLLRAISWKES
                     RYRVNAIGINPITGYGSGLMQVDSQHFNELARYGIKPEHLTTDPCMNIYTGAYYLAIA
                     FKKWGVSWVAVGAYNAGFRKTERQNQRRLAYASDVYRIYTGIKSSKGIRLPATKKSLS
                     EINSVQNN"
     gene            complement(1048..1869)
                     /locus_tag="N7B39_002294"
     CDS             complement(1048..1869)
                     /locus_tag="N7B39_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HGU2422266.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMHFVPSVFSGDKHESRSERY
                     TYIPTINVINKLRDEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGKEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTENMEAMKEIHLNGDEQYLFGRAALMVRYEDENKTPVTPEQIITPRRWEDKQNDLW
                     TTWQRVQENMIKGGLSGKSASGKNTRTRAITGINGDIRINKALWMIAEQFREWKS"
     gene            complement(1979..2275)
                     /locus_tag="N7B39_002295"
     CDS             complement(1979..2275)
                     /locus_tag="N7B39_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2422267.1"
                     /translation="MSAFSFNTTYQPTGDQQKDAIAQIGIMQNRAVQANLAYQSCRDS
                     GALFKVLHQVNNELHDLLDSLENHTPLVRKNADELIALLLLFTRQVGQSRTDLI"
     gene            2572..3022
                     /locus_tag="N7B39_002296"
                     /pseudo
     CDS             2572..3022
                     /locus_tag="N7B39_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3222..3464)
                     /locus_tag="N7B39_002297"
     CDS             complement(3222..3464)
                     /locus_tag="N7B39_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001398403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2422268.1"
                     /translation="MGYITYVTDQRPGEPDILTGNTFADLEICDSDGHLLLKVSAPEV
                     GWTHESLNLVQPQEVQEGNDAFDAYLNGIWIGSTEV"
     gene            complement(3523..3846)
                     /locus_tag="N7B39_002298"
     CDS             complement(3523..3846)
                     /locus_tag="N7B39_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001316561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2422269.1"
                     /translation="MGRNMSAARTDGFIRNIHSRNPFDVIRADVVISRLEKQAHWGCG
                     LHYEIYEANLFDMAMNHLSRLPLKDRPVFIGAAARKGLFLTMEETRLAREAKDALMAE
                     LALEY"
     gene            4142..4502
                     /locus_tag="N7B39_002299"
                     /pseudo
     CDS             4142..4502
                     /locus_tag="N7B39_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4740..4862)
                     /locus_tag="N7B39_002300"
     CDS             complement(4740..4862)
                     /locus_tag="N7B39_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004118124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HGU2422270.1"
                     /translation="MVCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYESGGK"
     gene            complement(4807..4959)
                     /locus_tag="N7B39_002301"
                     /pseudo
     CDS             complement(4807..4959)
                     /locus_tag="N7B39_002301"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5431 family protein"
     gene            complement(5109..5423)
                     /locus_tag="N7B39_002302"
     CDS             complement(5109..5423)
                     /locus_tag="N7B39_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="theronine dehydrogenase"
                     /protein_id="HGU2422271.1"
                     /translation="MKWRYSLRWKLPSPCPGKEVLVTAEVEAGQPAPASVMSRWVAGA
                     GYAVCLDFISDRPVRRWSEERKAAVRRRNLEKRINRHAPLFADELIARELAERPDYFQ
                     GK"
     gene            complement(5420..5929)
                     /locus_tag="N7B39_002303"
     CDS             complement(5420..5929)
                     /locus_tag="N7B39_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001674866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2422272.1"
                     /translation="MQNARHPKTHRELCELACRFLRNNGFKVVFSDRFHAWTSCGEHP
                     DALGFRNGASCLIEVKCSRSDFLADRKKVFRAEPEKGMGDWRFYLCEPGVITVDDLPA
                     GWGLLYVINGRVRKVHGWPGNHQWVVTVAKPFRANKQAECDYLFSALRRLEIRAVTEN
                     GTDADRVAV"
     gene            complement(5929..6648)
                     /locus_tag="N7B39_002304"
     CDS             complement(5929..6648)
                     /locus_tag="N7B39_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HGU2422273.1"
                     /translation="MTARQNALVPLNAEQQAAWRAVAETEKRRHQGNTLAEYPYAGAF
                     FRCLNGSRRISLSDLRFFMPSLTAEELRGNRSQWLYAVDVLIETQGEVCLLPLPGDAA
                     EQLFPSVRFRVRERSRHKSALVMQKYSRQQAREAEQKTRAYQALVAQAEIELAFHSPE
                     TVGSWHARWSDRVAEHDLETLFWQWGERFPSLAGMERWQWQDMPFWQVITEAGMAARE
                     ASHAVREMERWMVPNKLREVA"
     gene            complement(6645..7079)
                     /gene="psiB"
                     /locus_tag="N7B39_002305"
     CDS             complement(6645..7079)
                     /gene="psiB"
                     /locus_tag="N7B39_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HGU2422274.1"
                     /translation="MKTELTLNVLQTMSVQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFSPFHERFHIVVCSPGEASATWMVILVSISGRPFRVI
                     RTQPSFRPETVNHILRLAASLDEAGYSTASIINLLVTEGGAA"
     gene            complement(7134..9101)
                     /locus_tag="N7B39_002306"
     CDS             complement(7134..9101)
                     /locus_tag="N7B39_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HGU2422275.1"
                     /translation="MSVTEFKAKTGRKSSRKSSKEQENALSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSAESVSELAESIKGVGLLQNLVVHALPGDRHGVAAGGRRLAALNMLA
                     ERNILPADWPVRVKVIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNTKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEHLREAEGWEWCAGRMEPVGSCREDAGTYRSLPEPEAVLTEAEE
                     ERLNELMARYDALENQCEESDLLEAEMKLIDCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDVADDAERTEQVRKTDAVEEISLPLLTKMSSERTLAVQAALIKQPE
                     KTVALMVWRLCCCVFDYCNTTLHPFGMHIEEYHRRLTSDAPSGKRGQAWLSLMQEKAR
                     LEALLPEGWQKDFTTFFTLDGQALMSLMVFCTACSVDGVQTRNCGHTSRSPLDSLESA
                     IGFHMRDWWQPTKANFFGHLKKPQIIDALNDAGLSGAARDAEKMKKGDAAEHAEFHMK
                     DNRWVPGWMCTPRPQAETETTEYRDNQADAA"
     gene            complement(9162..9395)
                     /locus_tag="N7B39_002307"
     CDS             complement(9162..9395)
                     /locus_tag="N7B39_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HGU2422276.1"
                     /translation="MSEYFRIFQGLPDGSFTREQAEAVAAQYRNVFIEDDQGTHFRLV
                     VRQDGTLIWRSWNFEDGAGYWMNRYIRDFGILK"
     gene            complement(9452..9979)
                     /locus_tag="N7B39_002308"
     CDS             complement(9452..9979)
                     /locus_tag="N7B39_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HGU2422277.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGSAPQQNAQAQPQPQQNGQPQSADTTKKGGAKTKGRGRKAAQPEPQPQ
                     PPEGDDYGFSDDIPF"
     gene            10281..10707
                     /locus_tag="N7B39_002309"
                     /pseudo
     CDS             10281..10707
                     /locus_tag="N7B39_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10793..11356)
                     /locus_tag="N7B39_002310"
     CDS             complement(10793..11356)
                     /locus_tag="N7B39_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HGU2422278.1"
                     /translation="MHSQLRERIRLLRARLDNAAPVAEIRAESQLFVTPAPVCDRLVT
                     LAEISNRDHILEPSAGTGAILRAIRDTAPEAMCDAVESNSGLVRYLRENFNGVRVQCG
                     DFMEWQPVQYYSRIIMNPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREELPRGTFAYTDVPTMIIRLRA"
     gene            complement(11403..12764)
                     /locus_tag="N7B39_002311"
     CDS             complement(11403..12764)
                     /locus_tag="N7B39_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HGU2422279.1"
                     /translation="MTLAAITMTAPEAASPVQMYRATYSPDDNKLRLYAASRLDPETY
                     KKVHDAGFRWAPKQALFVAPAWTPGREDVLLSLAGEIVDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDKYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     DGFREMTKAQWAALPRDCKAVRSVAETEDHGAYRYRRTMDNNFRLVSVYITDMKITEI
                     PQK"
     gene            complement(12817..13047)
                     /locus_tag="N7B39_002312"
     CDS             complement(12817..13047)
                     /locus_tag="N7B39_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2422280.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPAPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            complement(14019..14210)
                     /locus_tag="N7B39_002313"
     CDS             complement(14019..14210)
                     /locus_tag="N7B39_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2422281.1"
                     /translation="MNINTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVV
                     NQQAVFLGGHYILQGSRNR"
     gene            complement(14207..14629)
                     /locus_tag="N7B39_002314"
     CDS             complement(14207..14629)
                     /locus_tag="N7B39_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HGU2422282.1"
                     /translation="MYCTVKEIIREVLDTDVPDSECVFAVVLTRGDVRHIAQDWSLSD
                     DELETVMQRLDDAFEHGADVSVVHDVVRELMEEKHASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            complement(14676..15101)
                     /locus_tag="N7B39_002315"
     CDS             complement(14676..15101)
                     /locus_tag="N7B39_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HGU2422283.1"
                     /translation="MQYAKPVTLNVEECDRLFFLPYLFGQDFLYVEASVCALAKKMMP
                     EYEGGFWHFIRLPDGGGYMMPDGDRFHMVNGENWFDRTVSADVAGIILTSLVINRQLW
                     LYHDSGDAGLTHLYRMRDAQLWSHIEFHPECNAIYAALD"
     gene            complement(15517..16287)
                     /locus_tag="N7B39_002316"
     CDS             complement(15517..16287)
                     /locus_tag="N7B39_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2422284.1"
                     /translation="MNETLNALIYRHASNLLLAQGWPEETDVDQRNPKYPGWISIYVR
                     LDAPRLATLLINRHGGVLPPLLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVHDAVRSVSCRVAEDARRIRAALTTTGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDAENLPVVLDAILNRGARFSSVEMYLVSECVEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARSEIRSMADALAKIRK"
     gene            complement(16332..16850)
                     /locus_tag="N7B39_002317"
     CDS             complement(16332..16850)
                     /locus_tag="N7B39_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HGU2422285.1"
                     /translation="MAPIACSWRRIPKFWNWIPASKIEKETRMYGTCETLCRELAAQY
                     TENTPLMLVVWSPEEIQALADGMEISLTGHEIRTVLARLEDIPEEQRIESGISSAAVM
                     EIINNVSENRQVTVPAELLASLIQTAEQALWKREWAARDHGLAVPECVTRRQEVVNQA
                     RTLLKNNTHEND"
     gene            complement(16780..17001)
                     /locus_tag="N7B39_002318"
     CDS             complement(16780..17001)
                     /locus_tag="N7B39_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2422286.1"
                     /translation="MNYAGHEKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVER
                     LTRQTLYRANRLFMEAYTEILELDSCFKD"
     gene            complement(17002..17685)
                     /locus_tag="N7B39_002319"
     CDS             complement(17002..17685)
                     /locus_tag="N7B39_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HGU2422287.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDDWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQKPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DNWFEPEAA"
     gene            complement(17761..18066)
                     /locus_tag="N7B39_002320"
     CDS             complement(17761..18066)
                     /locus_tag="N7B39_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2422288.1"
                     /translation="MTETGGQPPVSFPTKDVAVLLFLLRRLTRRGINAACGQCPASGR
                     ARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGIALPSKRRRLRYSTVGLTRYRTR"
     gene            complement(18070..18972)
                     /locus_tag="N7B39_002321"
     CDS             complement(18070..18972)
                     /locus_tag="N7B39_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HGU2422289.1"
                     /translation="MHFSGEPAQIAEIKRLASGAVTPFYRRATNEGIQLFLAGSAGLL
                     QTTEDVQFEPCPGLTAAGRGVVSPENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDVRESITALFTPKRGDWCDIWSNEDVSVWWNRLCDNVLPEKTMPFDL
                     RMVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVVAALSRRFGCTLEHWYAEQGCNFCGWQRYERGELVDVLWGELEWSSP
                     TDDDELPEVTGPAWIVDKVAHYGG"
     gene            complement(19067..19294)
                     /locus_tag="N7B39_002322"
     CDS             complement(19067..19294)
                     /locus_tag="N7B39_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015589601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2422290.1"
                     /translation="MTRRGRNAACGQCPASGRARDGRFYRSRLASVTVYASPSPFSDE
                     RPSSRFRGITLPSNRRRLRYSTVGLTRYRTR"
     gene            19195..19401
                     /locus_tag="N7B39_002323"
     CDS             19195..19401
                     /locus_tag="N7B39_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2422291.1"
                     /translation="MSPTPAGSGKSARPVPGRTRDTDHRQRSCRAASAGAVKRGAQRH
                     LLSSAKQNPKTTHTKPNKTPKQDK"
     gene            19507..20469
                     /gene="parM"
                     /locus_tag="N7B39_002324"
                     /gene_synonym="stbA"
     CDS             19507..20469
                     /gene="parM"
                     /locus_tag="N7B39_002324"
                     /gene_synonym="stbA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HGU2422292.1"
                     /translation="MLVFIDDGSTNIKLQWQESDGTIKQHISPNSFKREWAVSFGDKK
                     VFNYTLNGEQYSFDPISPDAVVTTNIAWQYSDVNVVAVHHALLTSGLPVSEVDIVCTL
                     PLTEYYDRNNQPNTENIERKKANFRKKITLNGGDTFTIKDVKVMPESIPAGYEVLQEL
                     DELDSLLIIDIGGTTLDISQVMGKLSGISKIYGDSSLGVSLVTSAVKDALSLARTKGS
                     SYLADDIIIHRKDNNYLKQRINDENKISIVTEAMNEALRKLEQRVLNTLNEFSGYTHV
                     MVIGGGAELICDAVKKHTLIRDERFFKTNNSQYDLVNGMYLIGN"
     gene            20472..20822
                     /locus_tag="N7B39_002325"
     CDS             20472..20822
                     /locus_tag="N7B39_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001630992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HGU2422293.1"
                     /translation="MDKRRTIAFKLNPDVNQTDKIVCDTLDSIPQGERSRLNRAALTA
                     GLALYRQDPRAPFLLCELLTKETTFSDIVNILRSLFPKEMADFNSSIVTQSSSQQEQK
                     SDEETKKNAMKLIN"
BASE COUNT         4836 a         6006 c         5336 g         4661 t
ORIGIN      
        1 agtatagtta tactctacat cacacccttt cttacaaaat accacataaa aataacagta
       61 accatatgtt tttaaagaca tatcgtgcaa gataaaatct tgcgacgggt gtggggcttt
      121 gatgttaagg agcaggcata tgaaaattat caggccgggc aggattcagt attaacgggc
      181 attgccgttt ttgttaaaaa atggcactct ggcacaggta aagataataa tggaattttg
      241 cagatgaaga gcgttctgct tgttggatta ctcgcgctta cctctccggt cattgctcag
      301 gactgttttg aactggctgg aagagattac aaaatagacc ctgatttact aagagcaata
      361 tcatggaaag aatcccgtta tcgggttaat gccatcggta ttaatccgat aacgggatat
      421 ggcagcggac ttatgcaggt agactcccag cattttaacg aactggcccg ctacggaatt
      481 aagccggaac atctgacaac agatccctgc atgaacattt ataccggtgc ttattacctg
      541 gcaatagcct ttaaaaaatg gggcgtctcc tgggtggccg ttggtgcata taatgcagga
      601 ttcaggaaaa ccgaacgcca gaaccagaga cgtcttgcct atgcatcaga cgtttaccgg
      661 atttataccg ggataaaaag cagtaaaggc atccggcttc cggccacgaa gaaatcactt
      721 tcggaaatta acagcgtgca gaacaattaa tcttataaac attgtcaatt attggttatg
      781 tcgcggcagc gacatggaag gccacacaca aaaatatcat atgagtgaca atactgactg
      841 gccgaagccc cgcgaagcgg gaatccatct tatatatacc cggtaactgc gcagcctcct
      901 gccagaccat ttttcgcggg cacaatattt acagactacc ggaaagccag ttgatgaaat
      961 tgaggcataa cagacgacac agcaatgcct cagaatgata ctataagtaa agccccgaaa
     1021 aattttttcg gggctttact tatagtatca tgacttccac tccctgaatt gttcggcaat
     1081 catccataac gccttgttga ttcggatatc accattaata cctgtaatgg ctcttgtcct
     1141 ggtattttta ccggaggcac tttttcccga taatccaccc ttgatcatat tctcctggac
     1201 acgctgccat gtcgtccaga gatcgttctg tttatcctcc caacgacgag gggtaattat
     1261 ttgttcaggc gtcactggtg tcttattttc atcttcatac ctgaccatca atgcagccct
     1321 gccaaataaa tattgctcgt caccgttaag atgtatctct ttcattgctt ccatattttc
     1381 agtgacttta tcaaagacac cgagcacttc atacgctccc tcaataacct gcccgacaat
     1441 atcaccttta tgtggaacgc ggatttctcc aaaattattc ccgcacacca ggccatttgt
     1501 gcagacaaaa cgaaaaatcc cggggatcat ctgataactg gatgaaccat catgtgaatt
     1561 aagcagaata atttcaggaa cttctttccc attaatatgc ccttccctgc gaagacgtaa
     1621 catatgttta ctgtattcgc gacgccccaa atcacgaacc cgactctgac aggcaaagaa
     1681 cggctggaaa ccttcatcac gtaacttatt gatgacattg atggttggga tatatgtata
     1741 acgctcactc cgggattcat gtttatcacc agaaaagaca gatggaacaa agtgcattaa
     1801 ttcgtcatcc gttaacgggc gctcacggcg aatagaatta tatcgaccaa aacgggaagc
     1861 taatcgcata acagtaactc ctttatgaag gcataaaatt aataaaagtt ttttcgctta
     1921 atatcatccc cctcggttat cgttaacaaa aagacaataa ttccgagggg aaggaccatc
     1981 aaatcaggtc agttcgggac tgaccaacct gccgagtgaa aagcagtaac agcgcgatca
     2041 actcatcagc gttttttctg acaagaggag tatgattctc caggctatcc agtaagtcat
     2101 gcagctcatt attgacctgg tggagcacct taaagagtgc accagaatcc cggcagctct
     2161 gatatgcaag atttgcctga acagcacgat tctgcatgat cccgatttgt gcgattgcat
     2221 ctttctgctg atcgcctgtt ggctgataag tggtgttaaa agaaaatgcg ctcataattc
     2281 ctcctatatc tgatgttttc agcccccatc atctgttgag tttcgggagc cgcggttttg
     2341 acgttttgcg gggacctgcc gtttgttccc tccggtattc accatcacct gtttgagaac
     2401 cttcgccgca aggggcatca ctgccacggc ctgacgcacc agtccggggc agaacgcaaa
     2461 attttttacc gggttttagg taaaaaattt tgtgggctgt tcatttaccc tggacaggtg
     2521 cgtcaggttg tggctgtatc agcccccggc gacaggggct taaacaggtg aatggcaccg
     2581 gacacaacaa cgggcaggga cccgccgcaa cgacgtgaag tgcggtttgc gaaacggcgt
     2641 tgccccttac atcccggcgg ccttttgccg ggatgccagg agcgcaggcc acggcgcgac
     2701 aagggtggcg cgaccgttcc ggtcgctgca aacccggggt tttgcgtcag ggatggaagc
     2761 ccgccagggc ggagacagct ccgctggctc cgttcacgac agcccgcccc gccaggggga
     2821 cgcccggaaa aagcacagtc aggagaacaa caaaacagac agtaatcaga gcaacaggaa
     2881 aaagaagaac agtagcagca gcagtagcag tagcagtagc agtagcagga acaacaacat
     2941 catcagtaac agacaaatga caggcatgaa aagacagaag cacacagcag aacatccgcg
     3001 taatgaaagg ggcggcgcgt aagcgcggcc cctttcagcg ccaccggcgt gctgtctttt
     3061 cccggcgcgg gcagacaggc atttatcctc ccgcctgccc ggcgtcttca ggcccgcaga
     3121 caaataatca tctcggggca cgggtataag caaacatgcc gcgcgacagc tccccgggaa
     3181 tgcagaagac gccagcaacg tcttctgctg gcgtgatttg ttcagacctc tgtcgaaccg
     3241 atccatatac cattcagata agcatcaaat gcatcattcc cctcctgcac ttcctgcggc
     3301 tgaacaaggt tcaggctttc gtgtgtccag ccgacttcag gtgcagacac tttcagcaga
     3361 agatgcccat cagaatcaca aatctccaga tcagcaaacg tgttcccggt caggatatct
     3421 ggttcaccgg gtcgttgatc tgtaacatat gtgatatatc ccatccattc tctccacatt
     3481 aaatatttca gcactatccg ggacgacggt gcggcccgat tttcagtatt ccagagcaag
     3541 ttcagccatc agagcatctt tcgcttcccg tgccagcctg gtttcttcca tcgtcagaaa
     3601 aagccccttt cgtgcagcag caccaatgaa taccggacga tccttcagag gaagccggga
     3661 aagatgattc atcgccatat caaacaaatt cgcttcataa atttcatagt gcagaccaca
     3721 tccccaatga gcctgctttt ccagcctgct gatcaccaca tcagcccgga taacatcaaa
     3781 gggattgcgg gaatgaatat tacgaataaa gccatcggta cgtgctgctg acatatttct
     3841 gcccatcttt aatacacctc tgttttttgc ccccatcatc ttttgagttt cgggagccgc
     3901 ggttttgacg ttttgcgggg acctgccttt tgttccctcc ggtattcacc atcacctttt
     3961 tgagaacctt cgccgcaagg ggcatcactg ccacagcctg acgcacctgt cctgggcaga
     4021 acgcaaaatt ttttaccggg ttttaggtaa aaaattttgt gggctgttca tttaccctgg
     4081 acagtcagtg cggcaggttg tggctgtatc agcccccggc gacaggggct taaacaggtg
     4141 aatggcaccg gacacaacaa cgggcaggga cccgccgcaa cgacgtgaag cgcggtttgc
     4201 gaaacggcgt tgcgccttac atcccggcgg ccttttgccg ggatgcaggg agcgcaggcc
     4261 acggcgcgac agaggcggcg cgaccggaac ggtcgctgca agccaggggt ttcgcgtcag
     4321 ggatggaagc ccgccagggc ggagacagct tcgctggctc cgttcacgac agcccgctcc
     4381 gtcaggggga cgcccgggaa cgacataagc aggagaacaa caaaactgac aggaatcaga
     4441 gcagcagtaa tcaaaatcag agctggagga aaaagagaat aatcacaggg aaggtgatgt
     4501 gatttctgac aggtcataaa cagagaaggc gggggaaaag aacgggcgcc gcaggcgcac
     4561 gttcatcccc gttcccggag ggaaacggcc ttcctggcag tctggttgtt catgcccgca
     4621 caatgccagc aaggagaaag gggccaccgg cgaaccagca gcccctttat aaaggcgctt
     4681 cagtgcagtc agaccagcat cagccctgaa aaggcgggcc tgcgcccgcc tccaggttgc
     4741 tacttaccgc cggattcgta agccattgtt gccgtaactt ccctgtatcc gtccttcagc
     4801 cggacttcgc acaggcgatt ccgggtcagg agtgtgaata tcaacaacgt gcagcacacg
     4861 attaatacgc accagataag ggcgtttccc ggtagtttca tggtttactc ctccttgcaa
     4921 tttgcacggt aagaggccat actggtgttg gttagacata cagtgggcct cgtaggttaa
     4981 tgaaaattaa ctacggggct ttctgctttc tgccacacaa cacggtaaca aaccaccttc
     5041 acgtcatgag gcagaaagcc tcaagcgccg ggcacatcat agcccatata ccagcacgct
     5101 gaccacattc actttccctg aaaataatcc ggtcgttcag ccagttcccg cgcgatcagt
     5161 tcatccgcaa acagtggtgc atgcctgttg atacgtttct ccagattccg ccgtcgtacc
     5221 gcagccttac gttcttcgct ccagcggcgc accgggcggt cagaaataaa atccagacag
     5281 accgcatacc ctgctccggc aacccagcgt gacataacgg atgccggagc aggctgcccg
     5341 gcttcaactt ctgccgtcac cagcacctct ttgccaggac acggtgaggg taatttccag
     5401 cgcagggaat aacgccactt catactgcta ccctgtcagc gtctgtaccg ttctccgtca
     5461 cggcccggat ttccagccga cgcagtgcac tgaacagata atcgcattca gcctgtttgt
     5521 ttgccctgaa cggttttgca accgtaacca cccactgatg atttccgggc cagccatgta
     5581 ctttcctgac ccgcccgtta atcacataca acagtcccca gcccgccggt aaatcgtcca
     5641 cggtaatcac ccccggctcg cagagataaa aacgccagtc tcccatgccc ttttccggct
     5701 ctgcccggaa cacctttttc cggtcagcca ggaaatcaga ccgtgagcat ttcacctcaa
     5761 tgagacatga cgctccgtta cggaagccca gagcatcagg atgttcaccg caggacgtcc
     5821 atgcgtgaaa acggtcactg aacaccacct taaaaccgtt gttgcggaga aaacgacagg
     5881 ccagttcgca cagttccctg tgtgttttcg ggtgtctggc gttctgcatc acgccacctc
     5941 ccggagtttg ttcggcacca tccaccgctc catctcacgg acagcatgac tggcctccct
     6001 tgccgccata ccagcctctg tgatgacctg ccagaatggc atatcctgcc actgccagcg
     6061 ttccattccg gccagtgacg gaaaacgctc gccccactgc cagaacaggg tttccaggtc
     6121 atgctctgcc acccggtcac tccagcgtgc atgccagctt ccaactgttt ccggcgaatg
     6181 gaacgccagt tcgatttctg cctgagcaac cagcgcctgg tacgcacggg ttttctgctc
     6241 tgcctcccgt gcctgctggc ggctgtactt ctgcatgacc agtgcggatt tatggcgact
     6301 gcgttccctg acacggaacc gcactgacgg gaacaactgc tctgctgcat cgccgggaag
     6361 gggaaggagg cagacctcgc cctgtgtttc aatcagcaca tcgacggcat acagccactg
     6421 agaacggttg ccacgcagtt cttctgcggt cagggacggc atgaaaaatc gcaggtcaga
     6481 cagcgatatc ctgcggcttc cgttcagaca gcggaaaaac gcccccgcat acggatattc
     6541 cgccagcgtg ttaccctgat ggcggcgttt ttccgtctcc gccaccgccc gccatgcggc
     6601 ctgttgctct gcgttcaggg ggaccagtgc attctgacgg gcagtcatgc agcacctcct
     6661 tccgtgacca gcaggttgat gatgctggca gtggaatatc cggcctcatc cagactcgcc
     6721 gccagacgca ggatgtgatt caccgtttca gggcggaacg atggctgtgt gcgaatcacc
     6781 cggaaaggac gtccgctgat actgaccagt atcaccatcc aggttgcgct ggcctcaccg
     6841 gggctgcata cgacaatatg aaaacgctca tggaacgggc tgaaacgcac ctggacgggg
     6901 aaaaatccgc caaactcgct gccgtactcg ccgttcatcg tccagttgtc cggcgcatcc
     6961 agctcccgca tcacggcgtg tgtcagctca cggcgttcat cacttccggc agcccggata
     7021 tcttcatatt cctgtacact catggtctgt aatacattca gggtcagttc agttttcatc
     7081 acggtatctc ccttgctgcc gccccgtctc cggcggggcg gtgtggttgt tcatcaggcg
     7141 gcatcggcct ggttgtcacg gtattccgtg gtttccgtct ctgcctgtgg acgtggtgta
     7201 cacatccagc caggcaccca gcggttgtct ttcatgtgga actctgcatg ttcagccgca
     7261 tcgcctttct tcattttctc cgcgtcccgc gcagcaccgg acagtcctgc atcattcagg
     7321 gcatcgataa tctgcggctt tttcaggtgt ccgaagaagt ttgcttttgt cggctgccac
     7381 cagtcgcgca tgtgaaagcc gatggcgctt tccagcgaat ccagcggact gcgtgacgtg
     7441 tgaccacaat tgcgggtttg cacaccatcc acggaacagg cagtacagaa aaccatcaga
     7501 ctcatcaggg cctgtccgtc aagcgtgaag aatgtggtga aatctttctg ccatccttcc
     7561 ggtaacagag cctccagacg ggctttctcc tgcatcagac tcagccatgc ctgacctctc
     7621 tttccactgg gagcatcact tgtcagcctt ctgtgatact cttcaatatg catcccgaac
     7681 gggtggagag ttgtattgca gtagtcaaaa acacaacagc acaatcgcca gaccatcagt
     7741 gccactgttt tttcgggctg ctttatcagc gcggcctgga ctgccagcgt gcgttcggaa
     7801 gacattttcg tcagtagcgg cagactgatt tcctccaccg catcagtctt acgcacctgt
     7861 tccgtgcgtt cagcgtcgtc agccacgtca tcttcactgc gcaactgcac accacgctgg
     7921 acacatacgt tgccataacg ccaggacacc accacgccac ttccggcacg catctccggc
     7981 gtccacgccc tgaccttcgc catgcagtca atcagcttca tttctgcttc cagcaggtcg
     8041 gattcctcac actggttttc cagcgcgtcg taacgagcca tcagttcgtt caggcgttct
     8101 tcttctgcct ccgtcagcac cgcttccggc tccggcagac tgcggtatgt tccggcatcc
     8161 tcacggcagg aaccaacagg ctccatacgt ccggcgcacc attcccagcc ttctgcttcc
     8221 cgaaggtgtt cagcgacagc ctggagtttt tccagcaggg cagcatcgag cgcaacacag
     8281 tccacatagc caccctcgtc gtcgctgaac aaatcggtgc gcagttcatc tggcgagaag
     8341 gcatcagccc ccacaaagcg gaatttagtg ttccccgcca ccgccacttc actttcggtc
     8401 accagacgac gaatggtctg tacttccggt ttaccgcccc atcccgactg acaggcagct
     8461 tcaaacacct gcacctgacg cgcggtgtcg ttctccagcg ccagcgcctg acagtgctct
     8521 gtggtgatgc ggtcttctgc cagcgcatcg aggatgacag gcgcaaggtc tgccagtttc
     8581 agcattcgct gaacgtggcg gggtgaatag cccagcaaat caccgatttg tgcaggcgtt
     8641 ttgccttcct gcgccattgc acggaatccg gcaatctgtt cggcagggtg catatcccga
     8701 cgatgaccgt tttcagtcat tgatgcggca gtcgccagtt cctgcggaat aaccttcacg
     8761 cggacaggcc agtcagccgg aaggatgttg cgctctgcca gcatgttgag tgctgccagt
     8821 cggcgaccac ctgcggcgac accgtggcgg tcaccaggca gggcatgaac aaccagattc
     8881 tgtaacaggc caacgccctt gatggactcc gccagttcac tgacggactc tgcggaatac
     8941 ggcaccgtgc gcacattcag cggtgattta atcagtgagg ccagcggcac gctcacttcc
     9001 tccgtctgcg ccagcagggc agacagggcg ttttcctgtt cttttgacga tttacggctg
     9061 gatttacgac cagtttttgc cttaaactct gtaactgaca tgattattcc tcttacggtt
     9121 tagttaatgt ctgccggacg tgttcccgtc cggcacctct cttacttaag aatcccgaaa
     9181 tccctgatgt accggttcat ccagtacccg gcaccgtcct caaaattcca ggagcgccag
     9241 atcaacgtgc catcctgacg gacaaccagg cgaaaatgcg ttccctgatc atcctcgata
     9301 aagacattcc ggtactgtgc ggcaacggct tcagcctgtt cgcgggtaaa ggagccgtca
     9361 ggcagcccct gaaatattct gaaatattcg ctcatcccca tctctccggt gatgccccgc
     9421 ataggacggg gcggttatca cagtcagtcg ttcagaacgg gatatcatct gaaaacccgt
     9481 aatcgtcacc ctccggcggt tgcggctgag gctccggctg cgcggcctta cgtccacggc
     9541 cttttgtttt cgcgccacct tttttcgtcg tgtcagcact ctgtggctga ccattctgct
     9601 gaggctgcgg ttgcgcctga gcgttctgct gtggtgcact ccccagcatc tgcatggtgc
     9661 ccgtggtctt aacaagaatt tcagtgacgt aacgggtgat accgttatcc tcccagctac
     9721 gggtgcggag ttgcccctcg atgtagacct gcgcgccctt gcgcagatat tcaccggcca
     9781 cttccgcgag cttgccgaac agcaccacgc ggtgccattc cgtttgctcc cgcatctccc
     9841 ccgtctgttt gtcacgccag ctttctgacg tggccacctg caggtttgcc actgcgcccc
     9901 cgtttgggat gtaacggact tccggatctt ttcccagacg accgacaaga atgaccttgt
     9961 taatgccacg aactgccatg atgaatctcc ttctgttgat gttttcagcc cccatcatct
    10021 gttgagtttc gggagccgcg gttttgacgt tttgcgggga cctgccgttt gttccctccg
    10081 gtattaacca tcacctgttt gagaaccttc gccgcaaggg gcatcactgc cacggcctga
    10141 cgcacctgtc ctgggcagaa cgcaaaattt tttaccgggt tttaggtaaa aaattttgtg
    10201 ggctgttcat ttaccctgga cagtcagtgc ggcaggttgt ggctgtatca gcccccggcg
    10261 acaggggctt aaacaggtga atggcaccgg acacaacaac gggcagggac ccgccgcaac
    10321 gacgtgaagc gcggtttgcg aaacggcgtt gcgccttaca tcccggcggc cttttgccgg
    10381 gatgccggga gcgcaggcca cggcgcgaca gaggcggcgc gaccggaacg gtcgctgcaa
    10441 gccaggggtt tcgcgtcagg gatggaagcc cgccagggcg gagacagctt cgctggctcc
    10501 gttcacgaca gcccgccccg tcagggggac gcccggaaaa ggcataagca ggagaacaac
    10561 aaaacagaca gtaatcagag cagcagaaaa aagaagaaca gtagcagtag caggaacaac
    10621 aacatcatca gtaacagaca aatgaccgtt ctgaaaagac agaagccccc atcagaacgt
    10681 ccgcgtaatg aaaggggcgg cgcgtgagcg cggccccttt cagcgccacc ggcgcgcggt
    10741 cttttcccgg cgcggactga cgggcattta tcctcccgcc agccctgtgt catcaggcac
    10801 gcagacgaat aatcatcgtc gggacatcgg tataagcaaa cgtgccgcgc ggcagctcct
    10861 cgcggacgtc agaaaacggt aacagcttct cctgctggcg cggcccgttc agacagacgg
    10921 caaccagcac gccgcccgga cgcagcaggg aaaaggcccg caggatatgc cggatatcct
    10981 gcccgtggct gaacggcgga ttcatgataa tccggctgta atactgcacc ggctgccatt
    11041 ccatgaagtc gccacactgt accctgacac cgttaaaatt ttcccgcaga taccggacca
    11101 gcccgctgtt gctctccacc gcatcacaca tggcctccgg cgcggtatcc cgaatcgccc
    11161 gcagtatggc cccggtgccg gcagagggtt ccagaatgtg atcacgattg ctgatttccg
    11221 ccagcgtcac caggcgatca cagaccgggg caggtgtcac aaaaagctga gattcagccc
    11281 ggatttcagc aaccggtgcg gcgttatcca gccttgcgcg cagcagccgg atacgttctc
    11341 ttaactgact gtgcatgtca ttatctccgt catcccccgg ggatttcccg gggggatata
    11401 ccttatttct gtgggatttc cgtgattttc atgtcggtga tatacacact caccaggcgg
    11461 aaattattat ccattgtgcg gcggtagcgg tatgccccgt ggtcttctgt ctctgccaca
    11521 ctgcgcacgg ccttacagtc ccggggcagg gcggcccact gtgcctttgt catttcccgg
    11581 aaaccgtccc ccggatagtt gactaccggc ggacgcttcg cggcctggct ggcgacggca
    11641 gcttcttctg ccgatggtgc tttgtagtcc gtgatgcgat cgggcgtcac tttcatcgtg
    11701 ccgctgtacc cgagaaaact gtaattcggc gttgtgactg aactcaccgc cccgttgctt
    11761 ttgttcacgc ggatgatggt cagccactcg ccccggctga aaacctgtcc gcccggctca
    11821 aaatcctgtg tccgggtaac cacgccgccg ctttcgtcca gcatggcacg ctcatagatc
    11881 aggcggttct gatagtggtt aacccagcgt tgctgatgct ggatctgccg ttcatgacag
    11941 cggatcgcaa tttcacgggc ctgctccgtg gtgatgatgt catcatccag ggccgaccat
    12001 aacgatcgac tcccttcata ctggcttttt tctgccgggc gcgggtactt atccagcggg
    12061 aaacaggcgc tgatatggtc atgactgctg atgagttttg ccatattcag atccagcgat
    12121 tcagcccgcc acatcgtcag atatttctgc gactgtgcga tggtcttttc agccttgcgc
    12181 aaatcagctt cgattttttt gatacgacgc cagcgaacgt ccggacgttc tttatacttc
    12241 gcgtgaagca gtgccgaccg cgcccgctct tcccagtatt ccgcacgttc aaagagcatc
    12301 acggcacgtt tcatgccgtt ttcaatacgc tgcgcatcac gacgggcgcg gcgttcgctg
    12361 tgatgcccca caagaatggg ctgaccgggc gggatcatcg cggccagtct ttccacttca
    12421 tcaagtgcct gtgcagattc actggcccgc ttcccactgt atccggtaaa ccgctccgcc
    12481 cgtgcttcct gacgttcagc aagcgtgctg tcttcatcca caatctctcc ggcaagtgag
    12541 agaagtacat cttcccggcc aggtgtccag gctggagcca caaacagcgc ctgtttgggt
    12601 gcccagcgga aaccggcatc atgcactttt ttatacgtct ccgggtcaag acgtgacgcg
    12661 gcatacaggc gcagtttgtt gtcatccggt gagtaggtcg cgcggtacat ctgcacaggg
    12721 ctggcagctt ccggggcggt catggtgatt gctgctaatg tcataaaatg ttctccttcc
    12781 gtctgttgcc gccccatcgc ggggcggctc acctcatcag ttaaaagcgc gtttcagttc
    12841 gagtgtgcgg agcacctgcc cgaaggccgt gtttttcatg gcggggatgt gtacaccacg
    12901 cccccggtga aaactccggg ctatgtgtcg cccgaaggct ttcagcacgg ggtcaatttc
    12961 cggcgcgggg atgtcttcgc gtctgaaacg gggatgacgg cggggcttta cagccgctgc
    13021 ggaaggtgaa acaatactta ctgtcataaa tgcatcctta tgactgttga atggaaccgc
    13081 agaaactgct cgaacagttt ttgcggtgtg gattaagcag gtcctccccg gcctgctttt
    13141 ttcatatctg cgtctgacgt tctccggtgt attcctcctt ctgttcctgc gttctcatta
    13201 cctgccagcc ggatgactga cagaacaaat aaatgcatat tgccggagcc tgaaataaat
    13261 cctgattttt aaagaacatc acccgttgag tttcgggagc cgcggttttg acgtttcgcg
    13321 ggaacctgct gctgtattcc ctgtggtatg aacttcaccc gtttgagaac cttcgccgca
    13381 aggggcatca ctgccacggc ctgacgcacc tgtcctgggc agaacgcaaa attttttacc
    13441 gggttttggg taaaaaattt tgtgggctgt tcatttaccc tggacagtca gtgcggcagg
    13501 ttgtggctgt atcagccccc ggcgacaggg actcatacgg gtgtgtggca cccggcaggc
    13561 aatgagcagg gacccgccgc aacgacggag agcgcgtgcg cgaaacggcg ttgcgcctta
    13621 catcccggcg gccttttgcc gggatgccgg gagcgcaggc cacggcgcga caagggtggc
    13681 gcgaccggaa cggtcgctgc aagccagggt ttcgcgtcag ggatggaagc ccgccagggc
    13741 ggagacagct tcgctggctc cgttcacgac agcccgcccc gacaggggga cgcccggaaa
    13801 agccataaac aggagaggaa cggaaagagg aagaagaaga acagtaacag gcacggtaac
    13861 cacagaaaaa taacatcaga aaaaaacatt ccggacacat cacaaacggg aagggtaacg
    13921 ggcgacgcgc cagcgtggcc cgcttccctt ccggcaggcg tcagcctgtc aggggttttc
    13981 gcgcgggctg acaggcggcc tccaccgcct gccctgtatc acctgtttct actgccctga
    14041 aggatatagt gaccgccgag gaaaaccgcc tgctgattca ctacaaaatc gcagatttca
    14101 tcaacaatct gcgcggtggt gagcggtttc tgccccattt cacgacgccg cgcattaatc
    14161 acggctttgt aattgcgcag aatttcgcgg gtttccgtgt tgatattcat gacatgtgct
    14221 ccccgtccag cgcctgtaac acaacgtcca ttgcttcgcg ttcttccctg acgaacgcat
    14281 caccgtcgcc cccgttctca ctcccgacgg catacaggcg cttcatttca ctgcctgcca
    14341 gcgccatcac tttttccagc atcaccgccg ggactgtcac ctgacggctg gcgtgctttt
    14401 cctccatcag ttcacgaaca acgtcgtgaa caacgctgac atccgcacca tgctcaaagg
    14461 catcgtccag ccgctgcatg acggtttcca gctcatcgtc tgacaggctc cagtcctggg
    14521 ctatgtgacg cacatcccca cgggtcagca ctacggcaaa aacgcattca ctgtccggca
    14581 cgtctgtatc cagcacttcg cggataattt ctttaacagt acagtacatg ttctcgtctc
    14641 ctttacaggg gcggcattgt cgccccgtcc gttaatcagt ccagcgccgc gtaaatcgcg
    14701 ttgcattcag ggtgaaattc gatgtggctc cacaactgcg catcacgcat cctgtacagg
    14761 tgtgtcaacc ctgcatcccc gctgtcgtgg tacagccaca actggcggtt aatcacaagg
    14821 gaggtaagga tgatgcctgc gacatcagca ctgaccgtgc ggtcaaacca gttttcaccg
    14881 ttcaccatgt ggaaacggtc gccatccggc atcatgtaac cgccaccgtc cggcaggcgg
    14941 atgaagtgcc agaatccgcc ttcatattcc ggcatcattt ttttcgccag cgcgcacaca
    15001 gacgcttcga catacagaaa atcctggcca aacaggtaag gcagaaaaaa cagacggtcg
    15061 cactcttcaa cattcagagt gacaggtttc gcatattgca tgatgttttc ctccgttgtt
    15121 cacatattcc gcgacaccgg gcggtgccgt gcctccatct ttgccgggcc tgcagggttg
    15181 caagggcgca agggaagccg cagcctgacg cgcacggctg acgggagttg gagggtgaga
    15241 ccgcgaaatc tgagcgggtg aacgaccgga aaatcacggc agacgggaag gagggaccgg
    15301 gtgaccgcag cggttcacct taccttgcaa cccgcccgca gaagcccggc tacgatggag
    15361 gcacggcaca gcccgcacac gacgctgagg ccacataact gaaaacccac aaggccatgc
    15421 cccgcatggc cgggtaaaac ggagcctgcg gcgacatcct tttgctcttt ccggggaccg
    15481 gctggaaaag gcggagattt ttccccgcca gcgtcatcat ttccggattt ttgccagtgc
    15541 atccgccatg ctgcggattt cactccgggc ttcccggacc acctcccgca gaacaccacg
    15601 gtcaaaccag cggcggggcg gttcatccgg tatacgcagc acatcgcagg ccagaccact
    15661 ggacaggata tgctccacac actcactgac cagatacatt tccaccgacg aaaaacgagc
    15721 gccccggttc aggatggcat cgagcaccac gggcaggttt tcggcgtctt catcgagcca
    15781 gcaggggtga tcgctttcct tcacaaccag gcgaaagcgg cgcgtctggc gggtcagcaa
    15841 cgtctgaccg gttgtggtca gcgccgccct gatacgccgc gcatcttctg ccacccggca
    15901 actgacactg cgtaccgcat cgtgcaccgc atcaagaact gccctgattt cgtcttcggt
    15961 cagccattct ccggcatacg ggaaagacac ctgcgttccg tctgccggaa gtaccggcag
    16021 cgactgccac tgactgccgg acagaacaag ttccgccccg gttccggtca gtttctgaat
    16081 ggcagaggcc aggagcggcg gcagtacgcc gccgtgacgg ttgataagta acgtcgccag
    16141 ccggggcgca tccagccgca cgtaaatgct gatccagccc ggatacttcg ggttacgctg
    16201 gtcaacatcc gtttcttccg gccagccctg cgccagcaac aggttgctgg cgtggcgata
    16261 aatcagtgca ttcagcgttt cattcataat tcatgaactc cctgtatcgc cgcccggagg
    16321 cggcgataac atcagtcgtt ttcgtgtgtg ttgtttttca gcagggtgcg ggcctgatta
    16381 accacctcct gacggcgggt gacgcattcc gggacggcga ggccatgatc ccgggcggcc
    16441 cattcacgtt tccacaatgc ctgctcagcg gtctgaatca gggacgccag cagttcagca
    16501 gggacggtca cctggcggtt ttcgctcacg ttattgatga tctccatcac cgcagcggaa
    16561 gaaataccgg actcaatccg ctgttcttcc gggatgtcct ccaggcgtgc caggacggtc
    16621 ctgatttcat gaccggtcag agaaatttcc ataccgtcag ccagtgcctg aatctcttcc
    16681 ggggaccaga caaccagcat cagcggcgtg ttctccgtat actgcgctgc cagctcacgg
    16741 cagagcgttt cgcatgttcc gtacattctc gtctccttct caatctttga agcaggaatc
    16801 cagttccaga atttcggtat acgcctccat gaacaggcga ttggcgcgat acagcgtctg
    16861 gcgtgtcagg cgttcaacaa gcacatcgga atcaaagcga cagcgatgct ccatatcgtt
    16921 cagcctggcc cgaaggtcac acatggtgtt ggccacctcc gccacgtcgg cgcgaagttt
    16981 ttcgtgtccg gcatagttca tttacgcagc ctccggttca aaccagttat cattcgcggc
    17041 cccctgctgc atggcccgtt gcacggcggc cagtcgttgc tgtccggcac ggtgatactg
    17101 ctcaagcagc tcgataccga tataccggcg tccggactga agggcggcca cgcaggttga
    17161 accgctgcct gcaaacgggt ccagcacaat cgcgttcggg tgtgtgaagc tctcaatcag
    17221 cggttgcagg ctggtaacgg gcttttccgt cgggtgatgg cggttgcctg aatatttcca
    17281 gcccagcacg tccggtagcg gtttttgtgg cagacgtgga cggccttttg ccaggatgta
    17341 agcgcattcg tggcgatagc ccacatatgc ggccttcgat gtgtagtttt tggtgaagac
    17401 caggtgtcca accacgctga atcccgcatt tttccaggca gacataaagc gatcgacgcg
    17461 attccagcca tagaagctca ccattaacgc gtcttttttg agtacgcggt acatctcatt
    17521 acaggccggt tgcagccaat catcggtttt atcgccggca atggtacgcc cctgacggtc
    17581 acgaaaaccg acgaggtacg gcgggtcggt gaggatgaaa tcaatggcgt tatcagggat
    17641 acgggccata acatcgatgc agttaccgag gacaaaacgg gacataccaa actccttctc
    17701 ttacagaggg caccgggagg gcacccctgt ggtgcccgtt ccccgctgcc tgtgggctgt
    17761 tcaccgcgtc cggtaacggg tcaggccgac cgtggaatac cgcagccgtc gtctttttga
    17821 cggcaaggct atgccgcgaa agcggcttga agggcgttca tcagaaaagg gcgacgggct
    17881 ggcgtacact gtcaccgacg ccagccggga gcggtaaaag cgcccgtccc gtgcccggcc
    17941 ggacgcggga cactgaccac aggcagcgtt tatgccgcgc cgcgtcagcc ggcgcagtaa
    18001 aaagaggagc acggcgacat cctttgtcgg aaaagaaacg ggcggttgcc cgcccgtctc
    18061 cgtcatactt catccgccat aatgcgccac cttgtcgact atccacgcag gtccggtgac
    18121 ttccggcagc tcatcgtcat ctgtcgggga agaccattcc agttcccccc acagcacatc
    18181 aacgagctct ccacgctcat atcgctgcca gccacagaaa ttgcagccct gctcggcgta
    18241 ccagtgctcc agcgtgcagc cgaaacggcg gcttaatgct gcaacaacgt cgctttccgg
    18301 ctgacaccac ggcgtgtcga aatccacctg aatgaagttt tccccctgac tgctgatatt
    18361 caggtcatac ccacaaggcc acttcacgcc ataccgttct gtataccagt gatatgcaga
    18421 aggaacaccg ttcagaacac cgccattgaa gccgttcact tcaacatcca ggcgggtggg
    18481 cagaaccatc cgcaggtcaa acggcatggt tttttccggc agtacgttgt cacacagacg
    18541 gttccaccac accgatacat cctcgttact ccagatgtcg caccagtcac ctctttttgg
    18601 ggtgaaaagt gcggtgatgc tttccctgac atcatccggt aacccttccc agcgacgctg
    18661 tccagtaccg ctctgcagcc agagttcatg cagcatcagg cagttttgct catccagcag
    18721 tacaccgttc tgcaggtgtg tcagccagcg ggtgaacgcg atattttccg gcgatacaac
    18781 accacgtccg gcagccgtca gtccggggca cggttcaaac tgcacatctt cagtggtctg
    18841 cagaagtccg gcacttccgg ccagaaacag ctgaatacct tcatttgtgg cgcggcgata
    18901 aaacggtgtg actgcaccgc tggccagtcg tttaatctca gcaatctgtg ccggttcacc
    18961 agaaaaatgc atacgatttg agcaccagtt tggcatggga attctcctct caggagcacc
    19021 gggagggagc ccctgtggcg cccgttcccc gctgcctgtg ggctgttcac cgcgtccggt
    19081 aacgggtcag gccgaccgtg gaataccgca gccgtcgtct gtttgacggc aaggttatgc
    19141 cgcgaaagcg gcttgaaggg cgttcatcag aaaagggcga cgggctggcg tacactgtca
    19201 ccgacgccag ccgggagcgg taaaagcgcc cgtcccgtgc ccggccggac gcgggacact
    19261 gaccacaggc agcgttcctg ccgcgccgcg tcagccggcg cagtaaaaag aggagcccag
    19321 cgacatcttt tgtcatccgc caaacaaaac ccaaaaacaa cccacaccaa acccaataaa
    19381 acaccaaaac aagacaaata atcattgatt gatagttaaa atggggtaaa cttgacaaat
    19441 aaacccactt aaaacccaaa acacccccaa aaacacacca caaaaacacc ataaggagtt
    19501 ttataaatgt tggtattcat tgatgacggt tcaacaaaca tcaaactaca gtggcaggaa
    19561 agcgacggaa caattaaaca gcacattagc ccgaacagct tcaaacgcga gtgggcagtt
    19621 tcttttggtg ataaaaaggt ctttaactac acactgaacg gcgaacagta ttcatttgat
    19681 ccaatcagcc cggatgctgt agtcacaacc aatatcgcat ggcaatacag cgacgttaat
    19741 gtcgttgcgg tgcaccacgc cttactgacc agtggtctgc cggtaagcga agtggatatt
    19801 gtttgcacac ttcctctgac agagtattac gacaggaata accaacccaa tacggaaaat
    19861 attgagcgta agaaagcaaa cttccggaaa aaaattacat taaatggtgg ggatacattc
    19921 acaataaaag atgtaaaagt catgcctgaa tctataccgg caggttatga agttctacaa
    19981 gaactggatg agttagattc tttattaatt atagatatcg ggggcaccac attagatatt
    20041 tctcaggtaa tggggaaatt atcggggatc agtaaaatat acggagattc atctcttggt
    20101 gtatctctgg tcacatctgc agtaaaagat gccctttctc ttgcgagaac aaaaggaagt
    20161 agctatcttg ctgacgatat aattattcac agaaaagata ataactatct gaagcaacga
    20221 attaatgatg agaacaaaat atcaatagtc accgaagcaa tgaatgaagc acttcgtaaa
    20281 cttgagcaac gtgtattaaa tacactcaat gaattttctg gttatactca tgttatggtt
    20341 ataggtggtg gcgcagaatt aatatgcgat gcagtaaaaa aacacacact gattcgtgat
    20401 gaacgttttt ttaaaaccaa taactctcaa tatgatttag ttaacggtat gtatctcata
    20461 ggtaattaat gatggacaag cgcagaacca ttgcctttaa gctaaatcca gatgtaaatc
    20521 aaacagataa aattgtttgt gatacactgg acagtatccc gcaaggggaa cgcagccgcc
    20581 ttaaccgggc tgcactgacg gcaggtctgg ccttatacag acaagatccc agggcccctt
    20641 tccttttatg tgaactgctg acgaaagaaa ccacattttc agatatcgtg aatatattga
    20701 gatcgctatt tccaaaagag atggccgatt ttaattcttc aatagtcact caatcctctt
    20761 cacaacaaga gcaaaaaagt gatgaagaga ccaaaaaaaa tgcgatgaag ctaataaatt
    20821 aattcaatta ttattgagt
//
LOCUS       DBFIFI010000065        20838 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.065, whole genome shotgun sequence.
ACCESSION   DBFIFI010000065 DBFIFI010000000
VERSION     DBFIFI010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20838
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.065"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..862
                     /locus_tag="N7B39_002326"
     CDS             <1..862
                     /locus_tag="N7B39_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU2422294.1"
                     /translation="YRKIQKERYENGEIKLNTLKQKAAPLRVFDERFGTRPLDAITVK
                     DVVSVLEEYKARGHNRMGQIFRKVLIDVFREAQQTGDVPPGFNPAESAKKPQVRISRQ
                     RLTFDEWMMIYNAAEKDGYFLQRGMLLALMTGQRLSDICKMQFSDIRDGYLHVEQQKT
                     GTRIAIPLALRCDKLNLTLDDVVSSCRDCVLSPWLLHHHHAKGTAKRGGMVKPATLTV
                     AFKKARDSVDYNWRANGTPPSFHEQRSLSERLFREQGVDTKILLGHSNQKMTDIYNDA
                     RGKEWKKLVI"
     gene            complement(908..1546)
                     /gene="ompW"
                     /locus_tag="N7B39_002327"
     CDS             complement(908..1546)
                     /gene="ompW"
                     /locus_tag="N7B39_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HGU2422295.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            1786..1875
                     /locus_tag="N7B39_002328"
                     /pseudo
     CDS             1786..1875
                     /locus_tag="N7B39_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            1903..2646
                     /gene="yciC"
                     /locus_tag="N7B39_002329"
     CDS             1903..2646
                     /gene="yciC"
                     /locus_tag="N7B39_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HGU2422296.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGVFASIRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            2676..3215
                     /gene="yciB"
                     /locus_tag="N7B39_002330"
     CDS             2676..3215
                     /gene="yciB"
                     /locus_tag="N7B39_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HGU2422297.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            3320..3718
                     /gene="yciA"
                     /locus_tag="N7B39_002331"
     CDS             3320..3718
                     /gene="yciA"
                     /locus_tag="N7B39_002331"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HGU2422298.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATDALFKYVAVDPEGKPRALPVE"
     gene            complement(3758..4477)
                     /gene="tonB"
                     /locus_tag="N7B39_002332"
     CDS             complement(3758..4477)
                     /gene="tonB"
                     /locus_tag="N7B39_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HGU2422299.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQQKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            4701..4997
                     /gene="yciI"
                     /locus_tag="N7B39_002333"
     CDS             4701..4997
                     /gene="yciI"
                     /locus_tag="N7B39_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HGU2422300.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            5298..6551
                     /gene="kch"
                     /locus_tag="N7B39_002334"
     CDS             5298..6551
                     /gene="kch"
                     /locus_tag="N7B39_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HGU2422301.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(6606..6779)
                     /gene="yciY"
                     /locus_tag="N7B39_002335"
     CDS             complement(6606..6779)
                     /gene="yciY"
                     /locus_tag="N7B39_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HGU2422302.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            6922..8382
                     /gene="cls"
                     /locus_tag="N7B39_002336"
     CDS             6922..8382
                     /gene="cls"
                     /locus_tag="N7B39_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HGU2422303.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFHSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKVYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            8417..8746
                     /gene="yciU"
                     /locus_tag="N7B39_002337"
     CDS             8417..8746
                     /gene="yciU"
                     /locus_tag="N7B39_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HGU2422304.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(8799..9803)
                     /gene="oppF"
                     /locus_tag="N7B39_002338"
     CDS             complement(8799..9803)
                     /gene="oppF"
                     /locus_tag="N7B39_002338"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HGU2422305.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(9800..10813)
                     /gene="oppD"
                     /locus_tag="N7B39_002339"
     CDS             complement(9800..10813)
                     /gene="oppD"
                     /locus_tag="N7B39_002339"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HGU2422306.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(10825..11733)
                     /gene="oppC"
                     /locus_tag="N7B39_002340"
     CDS             complement(10825..11733)
                     /gene="oppC"
                     /locus_tag="N7B39_002340"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HGU2422307.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(11748..12668)
                     /gene="oppB"
                     /locus_tag="N7B39_002341"
     CDS             complement(11748..12668)
                     /gene="oppB"
                     /locus_tag="N7B39_002341"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HGU2422308.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(12754..14385)
                     /gene="oppA"
                     /locus_tag="N7B39_002342"
     CDS             complement(12754..14385)
                     /gene="oppA"
                     /locus_tag="N7B39_002342"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HGU2422309.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            complement(15123..15770)
                     /gene="ychE"
                     /locus_tag="N7B39_002343"
     CDS             complement(15123..15770)
                     /gene="ychE"
                     /locus_tag="N7B39_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HGU2422310.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(15801..15854)
                     /gene="ychE"
                     /locus_tag="N7B39_002344"
     CDS             complement(15801..15854)
                     /gene="ychE"
                     /locus_tag="N7B39_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HGU2422311.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            16247..18922
                     /gene="adhE"
                     /locus_tag="N7B39_002345"
                     /gene_synonym="adhC"
     CDS             16247..18922
                     /gene="adhE"
                     /locus_tag="N7B39_002345"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HGU2422312.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            complement(19223..19840)
                     /gene="tdk"
                     /locus_tag="N7B39_002346"
     CDS             complement(19223..19840)
                     /gene="tdk"
                     /locus_tag="N7B39_002346"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HGU2422313.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            20444..>20838
                     /gene="hns"
                     /locus_tag="N7B39_002347"
     CDS             20444..>20838
                     /gene="hns"
                     /locus_tag="N7B39_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HGU2422314.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLD"
BASE COUNT         5460 a         5068 c         4852 g         5458 t
ORIGIN      
        1 ttacagaaaa attcaaaagg aaagatatga aaacggcgag atcaaactca acacgctgaa
       61 acagaaagcg gcccctctca gggtatttga tgaacgtttt ggcaccagac cgttagatgc
      121 cataaccgta aaagatgtgg tatcagtact ggaagagtac aaggccagag gacataacag
      181 aatgggacaa attttcagga aagtactgat cgatgttttc cgggaagctc agcaaacggg
      241 cgatgtcccg ccaggcttta accctgcaga atcggcaaaa aaaccgcagg tgcggatatc
      301 aagacagcga ctgacttttg atgagtggat gatgatttat aacgcagcgg aaaaggatgg
      361 ttacttttta cagcgcggta tgctgctggc actgatgaca ggccagcgcc tttcagatat
      421 ttgcaaaatg caattttcgg atatccggga tggttatctt catgtcgaac agcaaaaaac
      481 aggaacccgg attgccatcc ctctggctct gcgttgcgat aaattaaatc tcaccctgga
      541 tgatgtggtg tcatcctgcc gcgattgcgt tcttagtccg tggctattgc accaccatca
      601 cgcgaaaggg acagctaagc gcggcgggat ggttaagcca gcaacattaa ccgttgcatt
      661 taaaaaagcc cgggattctg tggattacaa ctggcgtgct aatggcaccc ctccctcttt
      721 ccatgagcag agatctttat cagagcgatt gttcagagag cagggggttg ataccaaaat
      781 tttgctaggc cattcgaatc aaaaaatgac cgatatttac aacgacgcac gcggtaagga
      841 atggaaaaaa ctggtcattt gatgaccagt tttgcagagg ggttttgcag aggttttgca
      901 gagaaaatta aaaacgatat cctgcggaga acataaacac ccacggatcc agtcgtaccg
      961 agtcttttac ggtagtaaca ccagatttgt acttagccgt ggtatcgata tccatgtacc
     1021 acactgacat gttaaccaac cagtcacggt taatcagata atcaaccccc acctgcccgg
     1081 cagctcccca ggaatctttc agactgagat cggaaagccc cgcctctttg ccatgatcgt
     1141 taaatccatt atcaaagaag gtggtgtagt taatacctgc cccaacataa ggacggaatt
     1201 tgctgctggc atcaccaaaa taccactgcg ccatcagtgt tggcggcaga tgatgaacgg
     1261 ttgcaatatc gccggtcgcc cgggtgccga ttttatggcg gaacggcgtc gctgccagta
     1321 attccacacc aatgttgtcg gtcgccatat aagtaaacgt caggcccagt tgagtgttat
     1381 tggtcacgct gaatccaccc agacttccta acgtaccacc agcaccttct gttggacgta
     1441 cggttgcaga acctgcacgc ataaaaaatt cgcctgcttc atgcgcaaag gcactgccag
     1501 agagaagagt tgttactgcc aaagccgcca ctgttaactt tttcatatcc gctccgtcgt
     1561 tatggttata aaaacgtgac gaatatacct acattggagt aacaaatgat cctacataga
     1621 tcacattaca tatgataatt caccatttat tgatctggat taatttagaa gttgcattga
     1681 gaatcctggt taacgtgatt taaatcaatt tttaaatatt ttcgctacgc aaatattaac
     1741 tcgttcgcca cttgccgcgg tttgcccacg cctgaaggcc agcaattacc tccgctttac
     1801 aaagatatgc ttttccatac aagacctgat gctgccaccc gaggcttaac cagggtacaa
     1861 ttgcccgcta attaacacct gcataaaact caaggagagt gcatgtctat cacggcgcag
     1921 tccgtatacc gtgacaccgg aaatttcttc cgtaatcaat ttatgaccat tctgttggta
     1981 tcgttgctat gtgcgtttat cacagtggtg ttagggcatg ttttctcacc cagtgatgca
     2041 cagcttgcgc agctcaatga cggcgtgccc gttagcggca gtagtgggtt gttcgacctg
     2101 gttcagaata tgtcaccgga acagcaacaa attttgctgc aggcttcagc ggcgtccact
     2161 ttttcaggat taatcggtaa cgccattctc gccggaggcg taatattaat tatccagctg
     2221 gtgtctgcgg gtcagagagt cagtgcgctg cgagctattg gtgccagtgc gccgatattg
     2281 ccaaagttat ttattctgat ttttctgact acccttttag tacagattgg catcatgctg
     2341 gtggtcgttc cgggaattat catggccata ttactggccc tggcaccggt gatgctggta
     2401 caggacaaaa tgggcgtttt tgcctcgatc cgtagcagta tgcggctgac ttgggcgaat
     2461 atgcgtctgg tggcacccgc agtactgagc tggttgctgg caaaaacact gttgctgctt
     2521 tttgcctctt cttttgccgc attaaccccg gaaattggtg ccgtactggc gaacaccttg
     2581 agcaacctga tttcagccat attgctcatc tatctgttcc gcctgtatat gttgattcgc
     2641 caataacctt aagcatttga ttacggaatc gtaaaatgaa gcagtttctt gattttttac
     2701 cgctggttgt ctttttcgcg ttttacaaaa tttatgacat ctatgcggct actgcggcgc
     2761 tgatcgtcgc cacggcgatt gtgcttatct atagctgggt tcgctttcgt aaggttgaga
     2821 agatggccct gatcactttt gttctggtgg tcgtcttcgg tggcttgacg ctgttcttcc
     2881 acaatgatga gtttattaaa tggaaggtta cagtcattta tgctctgttt gcgggagccc
     2941 tgttagtcag ccaatgggtg atgaaaaagc cgctaattca gcggatgctg ggtaaagaac
     3001 tcacgctgcc gcaaccggta tggtcgaagc tgaatctggc ctgggctgtt ttctttatcc
     3061 tttgcggtct ggcaaacatc tacatcgcat tctggctgcc gcaaaatatt tgggtcaact
     3121 ttaaagtctt tggcctgacc gcccttacct taatcttcac attgttaagc ggtatctata
     3181 tctaccgcca catgccgcag gaagataaat cctaaccaga ctgccagtcc tttggggctg
     3241 gcgctgtatc tcctctgttc tcctcaggaa aatcatagta gcatcgcgcc tgtgattttc
     3301 cttttaagtc ggttttacca tgtctacaac acataacgtc cctcagggcg atcttgtttt
     3361 acgtacttta gccatgcccg ccgataccaa tgccaatggt gacatctttg gtggttggtt
     3421 aatgtcacaa atggatattg gcggcgctat tctggcaaaa gaaattgccc acggtcgcgt
     3481 agtgactgtg cgggttgaag gaatgacttt cttacggccg gttgcggtcg gcgatgtggt
     3541 gtgctgctat gcacgctgtg tccagaaagg gacgacatcg gtcagcatta atattgaagt
     3601 gtgggtgaaa aaagtagcgt ctgaaccaat cgggcaacgc tataaagcga cagacgcatt
     3661 atttaagtat gtcgcggttg atcctgaagg aaaacctcgc gccttacctg ttgagtaata
     3721 gtcaaaagcc tccggtcgga ggcttttgac tttctgctta ctgaatttcg gtggtgccgt
     3781 taattttaaa caggatattc accacaatcc cactgcctgg cttacccggc tcataacgcc
     3841 atctgcgcat cgcatttttc acctcacgct caaacatgtt cgcaggcttg gctgagagga
     3901 tttgtacgtt atccacgcga ccatccggcg tgacgtcaaa tttaacttta acctgccctt
     3961 caatgcgcaa tgcctgtgct cgtgccggat actgcggctg attacggctt aatgcgcgtg
     4021 gtcctgaagc cacactggta accggcttgc tggttgcagc cgttgctgta cttgatgtcg
     4081 ggcgtgccgg tgccgtattt tcaaacggtg atgccggacg cgactctacg ggtttgacat
     4141 cgcgtttttg ctgctcctgt acctttttca ccggcttcgg ttttggctta ggtttcggct
     4201 tcggcttttc aatgaccacc ggtgcttctt ttggcggttc ggggatcggc tcaggttccg
     4261 gttctggctc taccaccggc tccggtggcg gctgaacggc ttgtggcggt tcgagatcag
     4321 caggcgcaac catcgtgaca gaaatcggct gcgcaggcgc aggtagttca ataacctgat
     4381 gtaccgaggt atagagcaga cccgccacaa cagcaccatg aatgcagacc gaaagtaacg
     4441 tcggccaggg gaagcggcga ggtaaatcaa gggtcattga agtcataatc atttcagtag
     4501 aaaaaccagg tctcgatttt aaatgcaaat agcaatcata ttcaataagg caagacgatc
     4561 aggatcagtg aaaatgtcat ttttggcgta attgacctta ctttatgcat ggtcttaaca
     4621 aagacatcat ctttccgttg cagatcttcg ccctttcaaa taacgtactt tacaactttc
     4681 ccgaacaagg agttgtgccc gtgttgtatg ttatttacgc tcaagacaaa gctgatagcc
     4741 tcgaaaaacg cctttccgtt cgtccggcac atttagcacg tttacagtta ctgcatgatg
     4801 aaggtcgttt gttgacagct ggtccaatgc cagcggtaga cagtaacgat ccaggggctg
     4861 cgggatttac tggctcaacc gttattgctg aatttgaatc gctggaagca gctcaggcct
     4921 gggccgatgc tgacccgtac gtagcggcag gagtctatga acacgtttca gtgaaaccat
     4981 ttaagaaagt gttctgagta gagatccaat atttaagggc acggtttttg caaccgtgtt
     5041 cttgtttggc ctttccttat aggtcggtca ttgcattcac caccacaagc agataataac
     5101 tttatctgta tttctttata tgatgtgagc atgttcagga aaaatgtcct ttcctgacgc
     5161 agagttgctc actcaaaacc tttgattact gcggataaag tcatctacta ttgtttaaca
     5221 cattcacgtg tcgcatgtat gttcagaaaa tgtgcacgtt aagataatta aacaaaattg
     5281 tggttacaga ggtatcagtg agtcactggg ctacattcaa acaaacagca acaaatttat
     5341 gggtgaccct acggcacgac attctcgcgc tggccgtctt tttaaatggg ttgcttattt
     5401 ttaaaacaat ctatggtatg tcggtcaatt tgcttgatat tttccatatc aaagcatttt
     5461 cagagttgga tctctccttg ctggcaaacg ccccactatt tatgctcggc gtctttcttg
     5521 tcctgaactc cattggctta ctgttccggg caaagctcgc atgggcaatc agtatcattt
     5581 tgttgttgat agcgctaatt tacaccctgc atttttatcc ctggctgaaa tttagtattg
     5641 gattttgcat ttttacgctg gtgtttttac tgatactgcg caaagacttc tcccacagta
     5701 gcgccgcagc cgggacaatt tttgcattta ttagtttcac gacattactg ttttactcaa
     5761 cctacggtgc gctttattta agcgaaggtt ttaatccgcg aatagaaagt ttgatgaccg
     5821 cgttctattt ttcgatagaa accatgtcaa ccgtcggcta tggcgatatt gtccctgttt
     5881 ctgaatcagc acgattgttc actatttcgg tcattatttc cggcattacc gtttttgcca
     5941 catctatgac ctcaattttt ggtccgctta tccgcggggg attcaacaaa cttgtaaaag
     6001 gaaacaatca tacaatgcat cgtaaagatc attttattgt ttgcggacat tcgattctcg
     6061 ccattaatac gatcctgcaa ctgaatcaac gcggacaaaa cgtaacggtt atcagcaact
     6121 tgcctgaaga tgatatcaag caacttgagc aacgcttagg cgataacgct gatgttatcc
     6181 ccggtgacag taatgacagt tctgtattaa agaaagcggg aatcgatcgt tgccgggcca
     6241 ttctggcgct gagtgataac gatgcagata acgcgtttgt tgtactctcg gcaaaagata
     6301 tgagcagtga tgtcaaaaca gttctcgccg tcagtgatag caaaaacctg aataagatta
     6361 agatggtaca tccggatatc attctctcac cgcaactgtt tggcagcgaa attctggcgc
     6421 gagttttaaa tggtgaagag attaataatg atatgctcgt ttcaatgttg ttgaactcag
     6481 gtcatggtat tttcagcgat aacgatgaac aagaaacgaa agctgacagt aaagaatcgg
     6541 cgcaaaaata gagtttaaag acggaagagt aaacttattg ggcaccgcaa tggtgcccag
     6601 tacatttaga tattgtagat cgcaaacaat aacgagttac gctgtttgtt tagaatgcac
     6661 ttcctgatgc tgtggatacg catatttcgg cgcgactgcc cctcaagcca acgcgattta
     6721 cgtcggctag cctgacgctg catgcgccag cgccccactt ccgttctact ccgcttcatg
     6781 ttaactactc tatgcaataa cagaatggtc attatacccc cgcccccgac gctgaccagt
     6841 gcttttcccg cgtttttatt tgccagatga atccatatgc gtaaactcat aacaatgcgc
     6901 tttcaaaagg atttctaatc tatgacaacc gtttatacgt tggtgagttg gttggccatt
     6961 ctgggatact ggttgctcat tgcaggcgta actttacgca ttctaatgaa acgacgcgca
     7021 gttccctccg cgatggcctg gctgttgatt atttacattc tgccgttagt cggaattatt
     7081 gcctatcttg ccgttggcga gctccattta ggcaaacgcc gcgctgagcg cgccagagcg
     7141 atgtggcctt ccaccgcaaa atggcttaac gaccttaaag cctgtaagca tatctttgcc
     7201 gaagaaaata gcagtgtcgc tgcgccatta ttcaagcttt gcgagcgtcg tcaggggatc
     7261 gctggggtca aagggaatca gctacaactg atgaccgagt cagatgatgt gatgcaggcg
     7321 ttaatccgcg acatccagct cgcgcgccat aatattgaga tggtgtttta tatctggcag
     7381 cccggcggca tggcggacca ggtggctgaa tcattaatgg cggctgcgcg acgcggcatt
     7441 cattgccgat tgatgctcga ctccgccggg agtgtagctt ttttccacag cccgtggccc
     7501 gagctaatgc gtaatgccgg tattgaagtg gtcgaagcct taaaggtcaa tctgatgcgt
     7561 gtgtttttac gccgtatgga cctgcgccaa catcgcaaga tgatcatgat cgataattac
     7621 atcgcgtaca ccggcagcat gaatatggtc gaccctcgct acttcaaaca agatgcgggc
     7681 gtagggcaat ggattgatct gatggcgcgt atggaaggcc ccatcgccac cgcgatgggg
     7741 attatttatt cctgcgactg ggagattgaa acagggaaac gtattctacc gccaccacct
     7801 gatgtcaata ttatgccgtt tgagcaggcc agcggtcata ccattcacac aattgcttct
     7861 ggccccggct ttccggaaga tctcattcac caggcattat tgactgcggc ttattcggcg
     7921 cgtgaatatt tgatcatgac cacgccctac tttgtgccaa gcgatgattt acttcatgcg
     7981 atttgcacgg cggcgcagcg cggggtggat gtcagtatta tccttccgcg aaaaaatgac
     8041 tcgatgctgg tcggctgggc cagtcgcgca ttctttacgg aactgcttgc tgctggggtt
     8101 aaagtttatc agtttgaagg cgggttactg cataccaaga gcgtgctggt cgatggcgaa
     8161 ctaagtctgg ttggcaccgt taaccttgat atgcgtagtc tgtggctgaa tttcgagatt
     8221 accctggcaa tcgacgataa aggttttggt gcagaccttg ccgccgttca ggacgattat
     8281 atttcgcgtt cacgtctgct cgatgcccgt ttatggctaa aacgtccatt atggcaacgt
     8341 gtcgccgagc gactgtttta cttcttcagc ccgttgctgt aaaacgtcgc caacagaggt
     8401 taaacaggta gtgactatgg atatggatct aaacaatcgc ctgactgaag atgaaacgct
     8461 tgagcaggcc tacgatattt ttctcgaact ggctgccgac aatctcgatc cagctgatgt
     8521 tctgctgttc aatcttcagt ttgaagagcg cggcggcgcg gagttattcg atccggcgga
     8581 agactggcag gaacatgttg atttcgattt gaacccagac ttttttgccg aagttgtgat
     8641 tggtctggcg gacagtgaag atggtgaaat caacgatgtt ttcgcccgca ttctgttatg
     8701 ccgcgaaaaa gatcacaaac tttgccatat catctggcgg gaataaataa tcactttcgg
     8761 cgtgaaaaag ggctgacagc tgtcagccct ttttgtgttt aaagcggatc gactttcagg
     8821 caagaaacgg cgtgtctgaa actcccctcc agcacaggac gtgttttggc acactccgga
     8881 ccggcaatcg ggcaacgggt acggaaaaca caaccggaag gcgggttgat cggcgacggt
     8941 aattcccctt ccagtaactg gatggttttg ttcttctcca gatccggatc aggtatgggg
     9001 actgccgaca tcaatgccct ggtgtaagga tgtagtggat tgtggtagac ctcatcatag
     9061 gtccccagtt ctaccgcatg gccgagatac atcaccaaca cacgatcgga aatgtgtttt
     9121 accacggcca ggtcatgagc gataaaaatt aatgacaatc ccatctcacg ttgcaattgc
     9181 tggagcaggt tgaccacctg tgcctgaatt gacacgtcca gcgccgacac cggctcatca
     9241 cagataatca gcttcggttc aagaataaga gcacgcgcaa tcccgatacg ctggcactgc
     9301 ccaccagaga actcatgcgg atagcggtta atcaggttag gcaataaccc gactttcagc
     9361 atcatcgcct tcacgcgctc gcgaacttcc tggcgtgaca ttttcggatg ataagtacgc
     9421 agtggttcag cgatgatctc gccgatggtc atacgcgggt ttagcgatgc caacggatcc
     9481 tggaaaatca tctgaatatc actgcgaacg gcacgccatt catcgggttt catgcccagc
     9541 aactctttac ctaaccaggc aacatgaccg tcggtcgctt taaccaggcc aatgatggcg
     9601 cgagcaaagg tggacttacc gcatcccgat tcccctacca cacctaatgt ttccccttca
     9661 tacaggcgaa gagttacacc atctacggct ttgagcgttt tcggcggttg ccagaaccac
     9721 tgtttgccat ctttgatttc aaagtgcact ttcagatcgg cgatttcgag gaggactttt
     9781 cttccttcgg ttacagcatt cataacagtt cctccaccgg tttaaagcaa gcacgcagac
     9841 ggccaggcgt aaactcttcc agcggcggtg cgctactaca aatttccatc gcatgcggac
     9901 aacgtggctg gaatgggcaa cctttcggta atcgcagcag gtttggcgga ttacccggaa
     9961 tagtcaacat tgtttcacct tccgcatcga gacgaggcac cgcgttgagc aaaccgatag
    10021 aataaggatg aacgggttga taaaagacat cgcgcgcgtt gccatattcc atcgtgcgcc
    10081 cggcgtacat taccagcact ttgtcgcaga tccccgccac cacgccaaga tcgtgggtga
    10141 tcataatgat ggcggtatta aattcccgct tcagttcatt caatagcgtc atgatctgcg
    10201 cctgtacggt gacgtccagc gcagtagtgg gttcatccgc aatcagcagc ttaggtcgac
    10261 atagcaatgc catcgcaatc ataactcgct gacgcatgcc gccagaaaat tcgtgcgggt
    10321 acattttcat gcgtttacgt gcttccggca tttttaccgc atcgagcatc cgcaccgact
    10381 cttcaaacgc ctcagctttg ctcatgttct tatgcagcat cagcacttcc atcaactgct
    10441 caccgacgcg catataggga ttcaacgaag tcattgggtc ctgaaaaatc attgagattt
    10501 gttcagcgcg cagtttattg agttcacgct ctggcaaatt gaggatttca cgcccattga
    10561 aggttgccga tccgccaatg cgtccgttgg cagccaacag gcccatcaac gcaaatgcag
    10621 tttgcgattt acccgaacca gactcaccta caatgcccag cgtttctccg gcacgtaggg
    10681 aaaaatttaa atcattgacc gccgtgacgt cgccgtccgg ggtactaaag gtgacacgca
    10741 aatctttcac gttcagcagt gcgtcagcct gttgttgtgc gagcggcaca gttgcagttt
    10801 caattacgct catggctgca ctccttaacg atctttcggg tcgagggcat cacgcaagcc
    10861 atcgccgata aagttgaaac aaaacagcgt caccacgagg aatcccgctg ggaacaacag
    10921 taaccatgga gagacttcca ttgagttcgc gccatcactc agcaatgccc cccagctgct
    10981 taacggctct tgcgtaccca accccaggaa gctaaggaaa gattcaaaga ggatcatgct
    11041 gggcaccagt agcgatgcgt agaccaccac cacaccgagt acgttcggca caatgtggcg
    11101 aataacaatg cccgacgtcg atacaccgcc aacttgtgcc gcctcaataa actctttgcg
    11161 cttcagactc agggtttgcc cacgcacaat acgagccata tccagccagg aaaccatgcc
    11221 tatcgccacg aaaatcagca ggatgttttg accgaaaaag gtcaccagca aaatgacgaa
    11281 gaacatgaat gggaaggagt tgaggatttc cagcaaacgc atcattaccg aatccacttt
    11341 accgcccaga taaccggaaa gcgaaccata aagtgtcccc acgaccactg ccaccagtgc
    11401 cgcagcaaca ccgaccatga gtgagatacg tccgccaatc gcaacgcgca caagcaggtc
    11461 gcgaccggat gagtcagtac caaagtagtg accggactcc atatccgggg cgctggacat
    11521 catcgcccag tcagtatcgt cataggcaaa ctgcgaaagc atcggtgcca ggattacaaa
    11581 taacgcgatc agcaccagca ctatcagact tgcaaccgcc gcacggttat gcataaaacg
    11641 tcgacgtgcg tcctgccaca agctgcgccc ttcgacctcc agcttttcac tgaaattttc
    11701 cagcgtctcg ctgtttttct tacttaacat catcgcgagc tccagtatca gtaacggatt
    11761 ttcgggtcga taaccgcata tagcacatcg acaatggcat taaacaaaat ggttaacgca
    11821 ccaaccagga tggtcaggct taacactaag gaatagtcac ggttcaatgc gccattaacg
    11881 aacagttgcc caatccccgg caaaccataa atggtttcga taaccataga accggtaata
    11941 atgccgacaa atgcggggcc catataggag agcacgggta acagagcagg ttttaatgcg
    12001 tggcgtaaaa tgatccgccg cataggtaac cctttcgccc gggcagtacg aataaagttg
    12061 gagtgtaata cttcaatcat agagccacgg gtaatacgcg caatactggc gatataggcg
    12121 agtgacaacg ccaccatcgg caatatcatg aatttaagcg ccccaccatt ccagccaccg
    12181 cccggcagcc aatgcaaaat gatcgcaaat atcatgacta ataatggcgc aaccacaaaa
    12241 ctggggataa caaccccggt cattgccagc cccatcacgg tatagtccca tttggtgttt
    12301 tgttttaatg cggcaataac gccagcacta acacccagta ttaccgcaag gaaaaatgct
    12361 gcggctccca gtttggcaga aacgggaaaa ctggatgcaa ccaggtcatt gaccgaataa
    12421 tctttatatt taaacgatgg accgaaatcg ccatgcgcca gttgtttcag gtagctgaaa
    12481 tactgtgtca tgatcggatc attaagatga tatttcgctt cgatattggc cattacttcc
    12541 ggcggtaaag tacgttcgcc ggtaaaaggg cttcccggcg cgaggcgcat cataaagaac
    12601 gaaatagtaa taagaataaa tagcgtcgga atcgcttcca gacagcgacg tagaataaat
    12661 tttaacattg ccctaccttc tggcctgtgc cttctgtaat gcgataaaaa tcagacaccg
    12721 tggagcagga cactcctgcc ccacgtattg ccatcagtgc ttcacaatgt acatattccg
    12781 ggtgtaggta ttatccagcg gatctttgcc ggtataacca ccaacccacg gtttcaccag
    12841 acgcgcattc acgtagtaat aaacaggaac aatggccgaa tccttatcca gctgttgttc
    12901 tgctttagtg tacagagctg tgcgctgcgc ctcgtcagtc actttcagcg tttccgccat
    12961 aatgctgtca aaggccgggc tcttataatg cgcggtattc atcgagctgt tcgacagcat
    13021 ggtgttcagg aaggaagttg gttcgttgta gtcagcacac cagcctgcac gggccacatc
    13081 aaaagtaccc tggtgacggg tgtcgaggaa cgttttccac tcctggttaa ccagtttgac
    13141 gtttacacca atgtttttct tccacaatga agaggcagca atcgccagct ttttatgcag
    13201 atcggaggtg ttatacaaca ggttgatggt caacggtttg tctgcggtat aacccgcttc
    13261 agccagcagt tttttcgctt cttcgttacg tttttcctgg ctccagccaa accattccgg
    13321 ctgagtcaat tttgcgccat cagtatacgg tggagtgtaa ccataggcgg gcatgtcgcc
    13381 ctgcgctttc actttattaa caatgatatc gcggtccata cccagtttca gtgcggtacg
    13441 cacacgcaca tcgttgaatg gcggtttctg gttgttaatt tcgtaataat aagtgcacag
    13501 gtatggatca acgtgaactt cgtccgggat ctcttttttc agcttctgga acaattcgat
    13561 cggcatgttg ttataagtca tgtcgatttc accactacgg tagcggttga catcggtaac
    13621 ttcagaagca atgggcaaat aggttacctg gttaataacg gttttcgcgt tgttccagta
    13681 ggttgggctg cgttcaagaa cgattcgttc gtttacgacc caatctttta aggtataggc
    13741 accgttggtg acgatattac caggctgggt ccatttttcg ccgaatttct cgatagcggc
    13801 ttttggcacc ggtgaagttg atgggtgaac aagtaattta tagaagtacg gaacgggttc
    13861 acttaaggtg acttctaatg tgtgatcatc aatagctttc acgccgagat cggtaatcgg
    13921 ttttttccct tcaagaattt catcaatacc ggcgatatgc ccatattgca gataactggc
    13981 atacggagaa gcggtgttcg gatcaacaga acgttgccag ctatacacaa agtcttgtgc
    14041 tgtgactggc gtgccatcag accatttcgc atctttacgc aaatggaagg tccagacttt
    14101 cgcgtcttta ttatcccagg attcagcgac gccaggtgcc ggatggccgt caagatcgct
    14161 gaccagtaag ccttcaaaca ggtctcggct gatattagac tccggaacac cttcaatttt
    14221 gtgcggatct aatgactgaa cttctgaacc attgttacgt accagcgttt gtttttccgc
    14281 cagtgtgacg cctgcgggta catcagctgc cagcgcgaca ttccctgcca ttagcgcagc
    14341 cagaacgcca gctgctacta aacttctctt ggtgatgttg gtcattgttt tttggactcc
    14401 ctcattataa ttactggtat taaccagcat gtgtaatccc ggattgggac cctgcacagg
    14461 ctcggagact ttctgctgtc aggttacttt atcgttacgg attgctatca ccgactttat
    14521 ttattctggt gattcagacg atcaccttat gtcgattcgt actgtgtctc ccctgtcgag
    14581 acacattatt ctttcgcgac gctctttaat tgttaattat tctcattacc aataaagaat
    14641 cgcctgacgg ttgcccggaa agtaccaaat gcggttctta ctcgccaata cattttgcaa
    14701 aattgttaag caattctctt ttattgaccg tatcagaatc tatagcttgc atccatgtct
    14761 taacaaacat ctttaaattc aatcaaatag agattggtta agcatgtcga tcttttctga
    14821 ttacagttcc agtagtgaaa tgcacaataa tttaacaatt gattattatt tagcactttc
    14881 ctctaccaaa ggcagtggaa ttactaatat catttcaatt attcttcagc aagcccagga
    14941 ttatgatgtg gcgaaaataa catgatgatg aggagaagca gttaaggaaa tccttataac
    15001 taactgtaat ttgagaagaa tatttttacc tattcctttt gcttttgata actgcgcggg
    15061 aacaaagtaa aaataatgac gaagtaaacg gagcatggca gctccgtttc atttgaaagg
    15121 aattaattaa gcaggccggg gaaaatcccc ttaataccag taacgataaa ttcaatcccc
    15181 aatgccatca gcaatagccc cataatacgc gtaatcacgt tgatgccggt ctggcgtaaa
    15241 acccgtacca gccacggtgc catgcggaac aatccccaac aacataaagc gaacaatgca
    15301 atagccacaa agaaaccaaa cagatagcta atgctgtgat aacgcgtacc ccagacgatg
    15361 gtagaactga tcgcccctgg ccccgccatc aacggcaacg ccaatggcac cacaccaata
    15421 ctttcacgta ccgcggtttc tgatttttct tgcttgttct gtttatcctc gccaagcttg
    15481 ccgctgatca tcgacatcgc tatcgtcacc accaggatgc ccccggcgat acggaacgaa
    15541 tcaattgata taccaaaaag ttgcagaatc gtgtcgccga gaaaaagcga gatccacaag
    15601 ataatggcca cagacaggtt ggctgtaagg ttagttttgt ttcgcgctgc cgctgtctga
    15661 taactggtca tgctgataaa gacgggaata atccctaccg ggttgaccag cgcaaataac
    15721 ccgatgaaaa atttgaagta aacgggaaaa tcaaaaaggg tctgaatcac ggttagctcc
    15781 gaagcaaaag ccggataatg ttagccataa ataaggttga aaagacgcgc tgacaatacg
    15841 ccttttgaca gcatttttca cctcctaact acttaaaatt gctatcattc gttattgtta
    15901 tctagttgtg caaaacatgc taatgtagcc accaaatcat actacaattt attaactgtt
    15961 atctataatg gcgaaaagcg atgctgaaag gtgtcagctt tgcaaaaatt tgatttggat
    16021 cacgtaatca gtacccagaa gtgagtaatc ttgcttacgc cacttggaag tgacgcatta
    16081 gagataataa ctctaatgtt taaactcttt tagtaaatca cagtgagtgt gtgcgcgagt
    16141 aagcttttga ttttcatcgg ttaagcaaat catcaccgca ctgactatac tctcgtattc
    16201 gagcagatga tttactaaaa aagtttaaca ttatcaggag agcattatgg ctgttactaa
    16261 tgtcgctgaa cttaacgcac tcgtagagcg tgtaaaaaaa gcccagcgtg aatatgccag
    16321 tttcactcaa gagcaagtag acaaaatctt ccgcgccgcc gctctggctg ctgcagatgc
    16381 tcgaatccca ctcgcgaaaa tggccgttgc cgaatccggc atgggtatcg tcgaagataa
    16441 agtgatcaaa aaccactttg cttctgaata tatctacaac gcctataaag atgaaaaaac
    16501 ctgtggtgtt ctgtctgaag acgacacttt tggtaccatc actatcgctg aaccaatcgg
    16561 tattatttgc ggtatcgttc cgaccactaa cccgacttca acagctatct tcaaatcgct
    16621 gatcagtctg aagacccgta acgccattat cttctccccg cacccgcgtg caaaagatgc
    16681 gaccaacaaa gcggctgata tcgttctgca ggctgctatc gctgccggtg ctccgaaaga
    16741 tctgatcggc tggatcgatc aaccttctgt tgaactgtct aacgcactga tgcaccaccc
    16801 agacatcaac ctgatcctcg cgactggtgg tccgggcatg gttaaagccg catacagctc
    16861 cggtaaacca gctatcggcg taggcgcggg caacactccg gttgttatcg atgaaactgc
    16921 tgatatcaaa cgtgcagttg catctgtact gatgtccaaa accttcgaca acggcgtaat
    16981 ctgtgcttct gaacagtctg ttgttgtcgt tgactctgtt tatgacgcag tacgtgaacg
    17041 tttcgcaacc cacggcggct atctgttgca gggtaaagag ctgaaagctg ttcaggacgt
    17101 tatcctgaaa aacggtgcgc tgaacgcggc tatcgttggt cagccagcct ataaaattgc
    17161 tgaactggca ggcttctctg taccagaaaa caccaagatt ctgatcggtg aagtgaccgt
    17221 tgttgatgaa agcgaaccgt tcgcacatga aaaactgtcc ccgactctgg caatgtaccg
    17281 cgctaaagat ttcgaagacg cggtagaaaa agcagagaaa ctggttgcta tgggtggtat
    17341 cggtcatacc tcttgcctgt acactgacca ggataaccaa ccggctcgcg tttcttactt
    17401 cggtcagaaa atgaaaacgg ctcgtatcct gattaacacc ccggcttctc agggtggtat
    17461 cggtgacctg tataacttca aactcgcacc ttccctgact ctgggttgtg gttcctgggg
    17521 tggtaactcc atctctgaaa acgttggtcc gaaacacctg atcaacaaga aaaccgttgc
    17581 taagcgagct gaaaacatgt tgtggcacaa acttccgaaa tctatctact tccgccgtgg
    17641 ctccctgcca atcgcgctgg atgaagtgat tactgatggc cacaaacgtg cgctcatcgt
    17701 gactgaccgc ttcctgttca acaatggtta tgctgatcag atcacttccg tactgaaagc
    17761 agcaggcgtt gaaactgaag tcttcttcga agtagaagcg gacccgaccc tgagcatcgt
    17821 tcgtaaaggt gcagaactgg caaactcctt caaaccagac gtgattatcg cgctgggtgg
    17881 tggttccccg atggacgccg cgaagatcat gtgggttatg tacgaacatc cggaaactca
    17941 cttcgaagag ctggcgctgc gctttatgga tatccgtaaa cgtatctaca agttcccgaa
    18001 aatgggcgtg aaagcgaaaa tgattgctgt caccaccact tctggtacag gttctgaagt
    18061 cactccgttt gcggttgtaa ctgacgacgc tactggtcag aaatacccgc tggcggacta
    18121 tgcgctgacc ccggatatgg cgattgtcga cgccaacctg gttatggaca tgccgaagtc
    18181 cctgtgtgct ttcggtggtc tggacgcagt aactcacgcc atggaagctt atgtttctgt
    18241 actggcatct gagttctctg atggtcaggc tctgcaggca ctgaaactgc tgaaagaata
    18301 tctgccagcg tcctaccacg aagggtctaa aaatccggta gcgcgtgaac gtgttcacag
    18361 tgcagcgact atcgcgggta tcgcgtttgc gaacgccttc ctgggtgtat gtcactcaat
    18421 ggcgcacaaa ctgggttccc agttccatat tccgcacggt ctggcaaacg ccctgctgat
    18481 ttgtaacgtt attcgctaca atgcgaacga caacccgacc aagcagactg cattcagcca
    18541 gtatgaccgt ccgcaggctc gccgtcgtta tgctgaaatt gccgaccacc tgggtctgag
    18601 cgcaccgggc gaccgtactg ctgctaagat cgagaaactg ctggcatggc tggaaacgct
    18661 gaaggctgaa ctgggtattc cgaaatctat ccgtgaagct ggcgttcagg aagcagactt
    18721 cctggcgaac gtggataaac tgtctgaaga tgcattcgat gaccagtgca ctggcgctaa
    18781 cccgcgttac ccgctgatct ccgagctgaa acagattctg ctggatacct actacggtcg
    18841 tgattatgta gaaggtgaaa ctgcagcgaa aaaagaagcc gctccggcta aagctgagaa
    18901 aaaagcgaaa aaatccgctt aatcagtagc gctgtctggc aacataaacg gccccttctg
    18961 gggccgtttt tttgtttacc caaagcaact tttccataaa ccgacagcat tagccttcat
    19021 catatttgcg acgatgtata acgcctaaac acagggatat tgtactttac aggtcacaag
    19081 tcaacgtcgg tgcttaagag ccctgtgagg cgtatagcgg cgttaaaaac tgccgagaag
    19141 ggtgtatagc ccggaagaag tgcgtaaaac gaactgacag gataaaagtg ccctgctcac
    19201 cctgtcagta aagaaattct tattaatcgt ggcgatgcct ttcctgaata gccgttaatg
    19261 agccgacttg taacgcctct ttatagtgtt tacggcatac agaaacgtat cgttcattac
    19321 caccaataac cacctgctca ccttcgttat aaggtctgcc tgcttgatca agacgcagca
    19381 ccatgcttgc tttacggcca caaaaacaga tggtttttaa ttcaaccagt ttgtcggacc
    19441 atgccagtaa gtattggctg ccaataaata attcacctcg aaaatcggta cgtaaaccat
    19501 aacaaagtac gggtatatcg agttgatcga caacctccga taattcatat acttgttgtc
    19561 tggttaaaaa ctggcattca tcaaccagta cgcaatgaat tgcctgctgc tcatgttccg
    19621 cacgaatctc atcaaataat gatgaatttt ggttaaataa ttttgcaggc gatgacaaac
    19681 ctatacgcga actgactttc ccggcaccaa agcgatcatc aatttctgcc gtatatacga
    19741 cagtgcgcat gccgcgttcc tggtaattgt atgaagattg caacaatgct gtagacttac
    19801 ccgcattcat tgcggaatag tagaaatata gctgtgccat cagccacagg ccctcaatga
    19861 tatgaaataa agttggctgg agtttatcat aattcgtcag tttttcagct caacagtatg
    19921 cacagaagtg gtatatgcgt tctcccttac gaagccttgc ataatccttc tgagctatca
    19981 ttacaactgc ctcgcttgtt ataagcgggt aaatgactgc tggtaactat tcacaatctt
    20041 taacctgttg cgcaagtaat agccctctgt tgacctccag gagatagtgc aatactaagt
    20101 ccctgctctt attgcgactg ttctactttt catcattcgc ttaataggga attctcgtaa
    20161 acacgactaa tacagaagac tgaaaggtcg tcagcctacg ataatctccc cataaaatgt
    20221 gacatgaatc aggaagtttt aacctcacgt gctgcgaaat catcggtaca aatagggcta
    20281 tatgccgcgt cttttctggc taattttatg aaaagatatt tattggcggc acaaaataaa
    20341 gaacaatttt gaattcctta cattcctggc tattgcacaa ctgaatttaa ggctctatta
    20401 ttacctcaac aaaccacccc aatataagtt tgagattact acaatgagcg aagcacttaa
    20461 aattctgaac aacatccgta ctcttcgtgc gcaggcaaga gaatgtacac ttgaaacgct
    20521 ggaagaaatg ctggaaaaat tagaagttgt tgttaacgaa cgtcgcgaag aagaaagcgc
    20581 ggctgctgct gaagttgaag agcgcactcg taaactgcag caatatcgcg aaatgctgat
    20641 cgctgacggt attgacccga acgagctgct gaatagcctt gccgccgtta aatctggcac
    20701 caaagctaaa cgtgctcagc gtccggcaaa atatagctac gttgacgaaa acggcgaaac
    20761 taaaacctgg actggccagg gccgtactcc agctgtaatc aaaaaagcaa tggatgagca
    20821 aggtaaatcc ctcgacga
//
LOCUS       DBFIFI010000066        20793 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.066, whole genome shotgun sequence.
ACCESSION   DBFIFI010000066 DBFIFI010000000
VERSION     DBFIFI010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20793
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.066"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            61..717
                     /gene="yobB"
                     /locus_tag="N7B39_002348"
     CDS             61..717
                     /gene="yobB"
                     /locus_tag="N7B39_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HGU2422315.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEHGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            741..1403
                     /gene="exoX"
                     /locus_tag="N7B39_002349"
     CDS             741..1403
                     /gene="exoX"
                     /locus_tag="N7B39_002349"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HGU2422316.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVHPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADMTGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(1400..3460)
                     /gene="ptrB"
                     /locus_tag="N7B39_002350"
     CDS             complement(1400..3460)
                     /gene="ptrB"
                     /locus_tag="N7B39_002350"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HGU2422317.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRNHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDLQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     RPAD"
     gene            complement(3669..4328)
                     /locus_tag="N7B39_002351"
     CDS             complement(3669..4328)
                     /locus_tag="N7B39_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF533 domain-containing protein"
                     /protein_id="HGU2422318.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLIGGGAVAGTVLWNKYKDKIRAAHQDEPQFGSQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(4655..5011)
                     /gene="yebF"
                     /locus_tag="N7B39_002352"
     CDS             complement(4655..5011)
                     /gene="yebF"
                     /locus_tag="N7B39_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HGU2422319.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(5078..5368)
                     /gene="yebG"
                     /locus_tag="N7B39_002353"
     CDS             complement(5078..5368)
                     /gene="yebG"
                     /locus_tag="N7B39_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HGU2422320.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            5502..6680
                     /gene="purT"
                     /locus_tag="N7B39_002354"
     CDS             5502..6680
                     /gene="purT"
                     /locus_tag="N7B39_002354"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HGU2422321.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYCESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(6736..7377)
                     /gene="kdgA"
                     /locus_tag="N7B39_002355"
                     /gene_synonym="eda"
     CDS             complement(6736..7377)
                     /gene="kdgA"
                     /locus_tag="N7B39_002355"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HGU2422322.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(7414..9225)
                     /gene="edd"
                     /locus_tag="N7B39_002356"
     CDS             complement(7414..9225)
                     /gene="edd"
                     /locus_tag="N7B39_002356"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HGU2422323.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(9460..10935)
                     /gene="zwf"
                     /locus_tag="N7B39_002357"
     CDS             complement(9460..10935)
                     /gene="zwf"
                     /locus_tag="N7B39_002357"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HGU2422324.1"
                     /translation="MAVTQTAQVCDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            11273..12142
                     /gene="hexR"
                     /locus_tag="N7B39_002358"
                     /gene_synonym="yebK"
     CDS             11273..12142
                     /gene="hexR"
                     /locus_tag="N7B39_002358"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HGU2422325.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            12270..13712
                     /gene="pyk"
                     /locus_tag="N7B39_002359"
     CDS             12270..13712
                     /gene="pyk"
                     /locus_tag="N7B39_002359"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HGU2422326.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGINYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(13843..14814)
                     /gene="lpxM"
                     /locus_tag="N7B39_002360"
                     /gene_synonym="msbB"
     CDS             complement(13843..14814)
                     /gene="lpxM"
                     /locus_tag="N7B39_002360"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310592.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HGU2422327.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRIAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(14934..16256)
                     /gene="mepM"
                     /locus_tag="N7B39_002361"
     CDS             complement(14934..16256)
                     /gene="mepM"
                     /locus_tag="N7B39_002361"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HGU2422328.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(16272..17204)
                     /gene="znuA"
                     /locus_tag="N7B39_002362"
     CDS             complement(16272..17204)
                     /gene="znuA"
                     /locus_tag="N7B39_002362"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HGU2422329.1"
                     /translation="MLHKKTLLFAALFAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSINGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            17283..18038
                     /gene="znuC"
                     /locus_tag="N7B39_002363"
     CDS             17283..18038
                     /gene="znuC"
                     /locus_tag="N7B39_002363"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HGU2422330.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            18035..18820
                     /gene="znuB"
                     /locus_tag="N7B39_002364"
     CDS             18035..18820
                     /gene="znuB"
                     /locus_tag="N7B39_002364"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HGU2422331.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTDGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(19066..20076)
                     /gene="ruvB"
                     /locus_tag="N7B39_002365"
     CDS             complement(19066..20076)
                     /gene="ruvB"
                     /locus_tag="N7B39_002365"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HGU2422332.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(20085..20696)
                     /gene="ruvA"
                     /locus_tag="N7B39_002366"
     CDS             complement(20085..20696)
                     /gene="ruvA"
                     /locus_tag="N7B39_002366"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HGU2422333.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
BASE COUNT         4946 a         5412 c         5186 g         5249 t
ORIGIN      
        1 attatgagta ttttctaaac cgcacccata atttgcagtc attttgaaaa ggaagtcatt
       61 atgtcgttct ggaaagttgc agcagcgcaa tatgagcctc gtaaaacctc actcaccgaa
      121 caggtagctc atcatctgga gtttgtgagg gccgccgcca ggcaacaatg tcagctgtta
      181 gtttttccat cgctttcttt actggggtgt gattattcgc gacgtgccct tcctgcccca
      241 cccgatctct cactgttaga cccgctttgt tatgccgcaa cgacctggcg aatgaccatc
      301 attgccggcc ttcccgttga atataacgat cgctttattc gtggaattgc ggtgttcgca
      361 ccgtggcgaa aaacgcctgg gatctaccat cagagtcatg gtgcatgtct gggccgtcgt
      421 tccagaacca tcacggtggt cgatgaacaa ccgcaaggta tggatatgga cccgacctgt
      481 tcactgttta cgacagggca atgtctggga gaacccgacc tgctggcatc ggcccgtcgc
      541 ttacaatttt tttcacatca gtactcgatt gctgtgctga tggcaaatgc ccgtggtaac
      601 agtgcactat gggatgaaca tggtcgtctc atcgttcgcg ccgatcgtgg ttcattattg
      661 ttagtcggtc agcgttcatc acagggttgg caaggcgata tcattccatt acgctaggct
      721 ttttcggcct ggagcatgcc atgttgcgca ttatcgatac agaaacctgc ggtttgcagg
      781 gagggatcgt tgagattgcc tctgttgatg tcattgacgg gaaaatcgtc aaccccatga
      841 gccacctggt gcaccccgat cgtcctatta gtccacaagc gatggcgatt catcgcatca
      901 ccgaagccat ggtcgccgat aaaccgtgga ttgaagatgt gatcccacac tattacggta
      961 gtgaatggta tgtcgcgcat aacgccagct ttgaccgccg cgtactgcct gagatgcccg
     1021 gtgagtggat ttgcactatg aaactggccc gtcgtttgtg gccagggatc aagtacagca
     1081 atatggcgtt atataaaaca cggaagctca atgtacagac gccgccgggc ctgcatcatc
     1141 accgcgcgtt gtatgactgt tatatcaccg ccgcgttgct tatcgatatt atgaacacct
     1201 ccggctggac ggcagaacag atggccgata tgaccggacg tccgtcgttg atgacgacct
     1261 tcacctttgg caaataccgt ggcaaagcgg tttccgacgt tgccgaacgc gatccgggct
     1321 atctgcgctg gttgtttaat aacctggaca gcatgagccc ggagctgcgt ttaacactga
     1381 aacattatct ggaaaatacc taatccgcag gcctagcggg taatgttccc tgcgccagcg
     1441 cgaccagaaa agcatattcc atcgctacgc cttcgtacga tttaaagcga ccagatttac
     1501 cgccatggcc tgagtccatg tcggtacaga gcaataaaag atggttatcg gttttcagct
     1561 cgcgcaactt agcgacccat tttgccggtt cccaatattg cacctgagaa tcgtgtaaac
     1621 cggtcgttac cagtaaatgt ggataagcct gtgcggtgac gttgtcatac gggctgtagc
     1681 ttttcatata ctcgtaatat tgcagatcct gcggattccc ccactcttca aactcaccag
     1741 tggtaagagg aattgattca tcaagcatcg ttgttacaac atcaacaaac ggtacctggg
     1801 cgataacgcc gtggaataat tccgggcgtt gattaattgc aacgcccatc aacatgcccc
     1861 ccgcactccc gcccatcgca taacaaagcg aaggagagcc atagcccagt tttaacaatg
     1921 catcgcaggc atcaagataa tcattaaacg tatttttctt cttcagaaat ttaccgtctt
     1981 cgtaccattg ttgccccagc tcaccgccgc cgcgaacatg gacaatggcg tagacaaagc
     2041 cacgatctaa caaactcaag cggctaaaac tgaaatcggc atcaatactt gcgccgtaag
     2101 aaccgtagcc atacaccagc agcgggttgt gtcctttgcg aaaatgattg cgatggtaga
     2161 ccaacgaaac cggaacttcg acaccatcac gggcgactat ccacaggtgt tcactgcggt
     2221 aattcgccgc atcaaaacca ggaacttctg tttgttttaa tacacgacgc tcaccggtat
     2281 ccatatccag ttcaaacaaa gtgtctggtg tggtcatgga agaataacca taacgcaatc
     2341 gcgcggtttc aggttctgga ttgtaggcaa tccaggtcac ataggctgga tcatcaaagg
     2401 caataccaat gacttcccgg gtcttgcggt taatttggcg caaactggtt aacccgcgct
     2461 gacgctcttc aaccaccagc cagtcggtaa acagcgtaaa cccttccagc atgatgtttt
     2521 cgcgtggcgg aattaactct tcccactgtt gctcatcacg catacgggta cggtataagc
     2581 caaagttttt gccgtggcgg ttggaacgca gataaaaacg atgctggtag tgatcaaggc
     2641 tgtattcgtg atctttgcgg cgcggcagaa aaacaaacgg ctcggcatct gccatttccg
     2701 cgtccagcag gcgaacttca ctggtggtgg cgctggccaa atgaatgact acatagtgct
     2761 ttgaggtcgt tttatgcagg ctgacgtaat aggtatcgtc tttttcttcg taaatcagtt
     2821 tatcttgcga tgctggcgta ccgatggcgt gacgccagac ctgataaggc agcagcgtca
     2881 ccggatgctt gcgaacatag tagaaagtcc aggagtcatt tgcccagaca aagctgggtt
     2941 caacgttatc cagcagttcc gggtaccagt tacctgtttc cagattacga aaacgaatgc
     3001 cgtactggcg tcgggaaaga aaatcttctg ccagcgccat aatggtgtta tcgggcgtaa
     3061 tcgccattcc gcccatcgaa taaaactcac tatgagccgc gcgcttgttg gcatcgagca
     3121 atatttccca ctcgtcccac tcttcactga acgccgattg acgctggtag atagcatatt
     3181 cacagcctgg ttcataaata tgccggtagc ggtagccatt tttgatgtag ggcgcagaaa
     3241 cttctcgttg cggaatgcgg tcgatgattt cctttaagat gcgatcctgc aaggcttgtt
     3301 gtgaggccat cacccgatga ccgtaactat tttcttgttg caggtagtcc aggacttccg
     3361 gctgagaacg cgtatcgtcc cgcagccagt agtaattatc gatgcgcgta tcgccatgaa
     3421 gcgtcatggc gtggggaatg cgggcggctt ttggtagcat gttattgttc tttctggttg
     3481 aaacatctta taagggtggc aaaactcacc ggggatgcaa gcgaaacagg gcagtcattg
     3541 cttagatgat gacaggtaat ggcgcgaata tcgaatgtta tgcagacaga gaaaatcagc
     3601 ctgttcaaat ggctgtgctg ttctggatag cccgaaatag tcaacttcag gctatccaga
     3661 gagcggaatt attccgccaa agtgcgtttt tgctgttcga gatcgcgttc aatgccttca
     3721 cgaacatcct gggggatttt cagcgcgtca cccagtgcat tcaggtaact gcgttccata
     3781 aaatggtcaa tatcaatagc cgcgcagctc aggaaataga tttccagcgc ctcttcttca
     3841 ttgcggaccc cggtagccag gcgttgtgga tccagcggtt gttcgattgc ctgctcaatg
     3901 agtacccgcc cctgctcttc cacgccggct tcacgcaatt gctggtcgat tgccgcacgt
     3961 tctttggcat caatatgacc atcactttta gcggcaaaga ctagcgcaag gatcaaacgt
     4021 tccgtacgct catccagcgg cgtactttgc gagccaaact gcggttcgtc ctgatgcgcc
     4081 gcgcgaattt tatctttgta tttattccac agcaccgtac ccgctaccgc tccgccgcca
     4141 atcagcaacg cgttggtgcc atatttcgta agaagtttac gtgctgattt atttgcgacc
     4201 agcagtccag ccagtccgcc taatgcgccc ggcactaaaa gtttgcccaa tccctgatcc
     4261 gcagacgagg aggtagaaga actgctttgc ccaagcaggg attgcagttg atttaaccag
     4321 ttagccatat ttgctcctca ataaccattt ttagtgcctg tagcatagag gatgaggatg
     4381 tcagaaaatg tatatcgaga caaaagatgc gcaaataagc ggggcggtag gccgttattc
     4441 aaagaaattc gcgccaggta aggtatttac tgtatatcag gtgataaggg tattttaggt
     4501 gaaagtagtt tgtcgttttg agtggcattg tttgatgctg attgcccgat gcgacgctgt
     4561 tgcgtcttct caggcctacg attgtggtgc gttctggggc ggacaaggca ctcgcgccgc
     4621 atccgccaac ggtcaatcga attatcagaa cccattaacg ccgctgatat tccgccattc
     4681 ccgctttgca gtccacgctg acctggtaat gaatatcggc acttttacca cgtacggtta
     4741 gcggtactga ccatttatca tctttaccct gaatgtcctg caaactgacc catgccacgg
     4801 gatcggcctg accgacaatt ttttgatcat ctgcccagcg cgccacgcga ttttgttgat
     4861 aatcacgttt tacgctcgcg gcaattccgg cagcatccag accttcacac tttgggaaag
     4921 tgaccgactt gctggtttca ttattggcag cgaaaactga tgcgcaggca gaaaccaaca
     4981 acagccctaa aaacgcccct ctttttttca tgtttttctc catagcacaa tgattcagga
     5041 gaaagcatgg tacaaattgt caggagcgca agttgcttca ggccgcgtgt gaggcatctt
     5101 cctcttcgga ttcagcaccg acgacctgtg gggacggtaa tttgccggtt ttcagaatgg
     5161 tgctcagcac atctttttgt tctgccagcc atagcgaaag ggcttcacgt tgctcgtctt
     5221 ccatttgcac tggagaattt gtcagccagg tgtcaagcag atccgccgta tcaagcattt
     5281 tgtcataagc atcggcttcc tttttgctgg taaacgacat tttctcttcg ccctcacgaa
     5341 tgactacgta tttaacttca accgccattt gcagcctctc ataataactg tgattctata
     5401 cagtatattt cttttcggtt gagagatcaa caccagcaat aaagacacac gcaaacgttt
     5461 tcgtttatac tgcgcgcgga attaatcagg ggatattcgt tatgacgtta ttaggcactg
     5521 cgctgcgtcc ggcagcaact cgcgtgatgt tattaggctc cggtgaactg ggtaaagaag
     5581 tggcaatcga gtgtcagcgt ctcggcgtag aggtgattgc cgtcgatcgc tatgccgacg
     5641 caccagccat gcatgtcgcg catcgctccc atgtcattaa tatgcttgat ggtgatgcat
     5701 tacgccgtgt ggttgaactg gaaaaaccac attatatcgt gccggagatc gaagctattg
     5761 ccaccgatat gctgatccaa cttgaagagg aaggactgaa tgttgtcccc tgcgctcgcg
     5821 caacgaaatt aacgatgaat cgcgagggta tccgtcgcct ggcggcagaa gagctgcagc
     5881 tgcccacttc cacttatcgt tttgccgata gcgaaagcct tttccgcgag gcggttgccg
     5941 ccattggcta tccctgcatt gtaaaaccgg tgatgagctc ttccggcaag gggcagacgt
     6001 ttattcgctc tgcagagcaa cttgctcagg catgggaata cgctcagcaa ggcggtcgcg
     6061 ccggagcggg ccgcgtaatt gttgaaggcg tcgttaagtt tgacttcgaa attaccctgc
     6121 taaccgtcag cgcggtggat ggcgtccatt tctgtgcacc agtaggtcat cgccaggaag
     6181 atggcgacta ctgtgaatcc tggcaaccac agcaaatgag cccgcttgcc cttgaacgtg
     6241 cgcaggagat tgcccgtaaa gtggtgctgg cactgggcgg ttatggtctg tttggtgtcg
     6301 agctgtttgt ctgtggtgat gaggtgattt tcagtgaggt ctcccctcgt ccacatgata
     6361 ccgggatggt gacgttaatt tctcaagatc tctcagagtt tgccctgcat gtacgtgcct
     6421 tcctcggact tccggttggc gggatccgtc agtatggtcc tgcagcttct gccgttattc
     6481 tgccacaact gaccagtcag aatgtcacgt ttgataatgt gcagaatgcc gtaggcgcag
     6541 atttgcagat tcgtttattt ggtaagccgg aaattgatgg cagccgtcgt ctgggggtgg
     6601 cactggctac tgcagagagt gttgttgacg ccattgaacg cgcgaagcac gccgccggac
     6661 aggtaaaagt acagggttaa acccggcaaa taaacgctac aaaaatgccc gatcctcgat
     6721 cgggcatttt gacttttaca gcttagcgcc ttctacagct tcacgcgcca gcttagtaat
     6781 gcggtcgtaa tcgcccgctt ccagcgcatc tgccggaacc agccaggaac caccgatgca
     6841 cagcacgctt ttcagcgcca ggtagtcacg gtagttagcc ggagaaatac cacccgtcgg
     6901 gcagaaacgg acctgggaga acggacccgc gatcgcctgc agggctttca cgccgccgtt
     6961 agcttcagcc gggaagaatt tgaactcttt caaaccgtag tccataccca gcatcagttc
     7021 ggaaacagtg ctgatccccg gaatcagcgg aatagtccct tcggtagcag ctttcagcag
     7081 cggctcggtc agacccgggc taattgcgaa ctgtgcaccc gcttcagtga cttctgccag
     7141 ctgctgtgga ttcagcaccg taccggcacc cacaatcgct tcaggcactt ctttggcgat
     7201 agcacggata gcgtcaactg cacactcggt acgcagagtc acttccagaa cgcgcacccc
     7261 accagcaacc aacgcttttg ccatcggcac cgcgtgttcc agttttttta ccacgataac
     7321 cggtacaacc gggccggtgg tcaggattga ttctgcactt gttttccagt ttttcatcag
     7381 agttttctct cgcctgatta caaatttgtc gtcttaaaaa gtgatacagg ttgcgccctg
     7441 ttcggcaccg gacagttttt cacgcaaggc gctgaataat tcacgtcctg ttcccacgcg
     7501 tgacgcgctc aggtcaggaa tgtgtggttc gcgagcagcc aattccgctt cgtctaccag
     7561 cagcgtcagt tcgcctgtct gtccattcac acgaatgatg tccccgtcgc gcacttttgc
     7621 cagcagcccg ccatcgtaag cttctggtgt tacgtggata gctgacggca ctttacctga
     7681 agcgccggag agtcgtccat cggtaactaa cgcaattttg aaacaccggt ccaataatac
     7741 accaagtggc ggcatgagtt tatgtaattc tggcattccg ttcgcttttg gcccctgatg
     7801 acggacaaca acgacacaat cgcggtccag caaacccgct tcaaaggccg gcataacgtc
     7861 atgctggctt tcaaaaacaa ccgctggcgc ttcaatcacc tggttctcaa ccggcacggc
     7921 agaggttttc ataaccgcac ggcccaggtt accgcttaac acttttgtcc caccatgatg
     7981 agagaaaggt tgttcgaagg aagcgatcac attgctgtcg agtgattttt ccgccccttc
     8041 ccgccagtcc agttcaccat tattcagcca tggttcaagg gtataacgag acagaccaaa
     8101 gcctgccacc gtattgacat cttcatgcag caggcctgct ttgagcagtt cacgcaccag
     8161 aaccggtacg ccacctgccg cctggaagtg gttaatatcg gccggaccgt tcgggtagag
     8221 acgtgccatc agcggtacaa catcagaaag gtcagagaag tcatcccagt taatctgaat
     8281 accggccgcg cgcgccatcg ccaccaggtg catggtgtgg ttagtggaac caccggtcgc
     8341 cagcagtgca acgataccgt tcaccaccac tttctcatcg atcatcttac cgatcggcat
     8401 ccattcatta ccattaccgg tcatgcgtgt aacctgacgc gcagcggcag cggtcaaagc
     8461 atcgcgcagc ggagaatccg gatgaacaaa agaagagcct ggcaactgca tccccataaa
     8521 ctccaccacc atctggttgg tgttggcagt accgtagaaa gtacatgttc ccggcgcatg
     8581 gtaagacgcg gcttctgact ccagtaaggc catgcggtcc actttacctt cggcataaag
     8641 ctggcgaata cgcacttttt ctttatttgg caaaccgctt gccatcggtc cagacggcac
     8701 aaacaccgca ggcaaatgac caaacgacag ggctgccatc gtcagacccg ggacaatctt
     8761 gtcgcacacc ccgaggaaca gagcaccatc aaacatgtta tgggacagcc cgacagccgc
     8821 agacatcgct atcacttcgc ggcttagcag cgacaattcc attccatcct gcccctgggt
     8881 gacaccatca cacatcgccg gaacaccgcc cgcaacctga ccaaccgcat tcgcttcatg
     8941 cagggcttta cgaatgattt ctggatagtg ttcataaggc tggtgagcgg agagcatgtc
     9001 gttataggag gtgatgatgg cgatattgtt acgcaacatg cttttcaaag aggctttgtc
     9061 ttctggctgg caggcagcga aaccgtgtgc caggttaccg catgccaact gcgaacgatg
     9121 aacggtcgac gttttcgctt gttctatccg ggcgagataa gcagagcgag tctcgcgcga
     9181 acgttcaatg attcgatttg ttacgcgtaa caattgtgga ttcataaagg ctcctgaaat
     9241 tgagttgtca gagcaggatg attcacaacg cgtttcattc agaggattta tgagtgaaac
     9301 gcctgtaacc ggagctcata gggcaaaaac gtttcagtca gtgtaataaa aaaagcctcg
     9361 tgggtgaatc cgcacgaggc ctgaaagtgt aaaaattgtt ctacaatctg tgcaagatca
     9421 tgttaccggt aaaataacca taaaggataa gcgcagatat tactcaaact cattccagga
     9481 acgaccatca cgggtaatca tcgccaccga ggcaacgggt ccccaggttc cggcctgata
     9541 cggtttcggc gcatcattgt ccatcgccca cgcctcagta atggagtcta cccatttcca
     9601 ggcttcttcc acttcatcgc gacgtacaaa cagtgcctga ataccacgca tggtttccag
     9661 cagcaaacgt tcataggcat ccgccagatg cgtctgatta aaggtttctg aatagctcag
     9721 atccagcttg gtgatttgca ggttatgttt gtggtcaagg ccaggaactt tattcagtac
     9781 ctggatatcc acgccttcat caggttgcag acggatagtc agtttattct gcggcagatc
     9841 ctgccacgac tctttaaaca gattcagttc aggtgttttg aaatagacca cgacttcaga
     9901 acatttggtc ggcagacgtt taccagtacg caggtagaat ggcacaccgg cccagcgcca
     9961 gttatcaatg tcgacgcgga tcgccacgaa ggtttctgta ttgctgctct tgttcgcgcc
    10021 ctcttcttcc agatatcccg gcactttttt gccctgggcg aagcccgcag tatattgccc
    10081 gcgtacggtt ttttcgcgta cgttggagcg gtcgatgcgg cgcagagact tcagtacttt
    10141 cactttttca tcgcggatgc tgtctgcgct caggtcagac ggcggagaca tcgcaatcat
    10201 gcaaagaatt tgcagcaggt ggttctggat catgtcgcgc atctgaccgg ctttatcaaa
    10261 atagccccag cgcccttcga tccccacttc ttctgccacg gtaatctcaa catgatcaat
    10321 ggtgcgattg tcccagttat tcacaaacag ggagttagca aaacgcagcg ccaacaggtt
    10381 cagcaccgtt tctttaccaa gatagtggtc gatacggtaa acctggcact cctcgaagta
    10441 ttcgccaacc tgatcattga tttcctgcga ggtcgccagc gacgtcccca gcggtttctc
    10501 catgactacg cgtgccggtt tagcattcag ttttgcctcg ccaagccctt tgcaaattgc
    10561 gccaaaagtg ctgggcggca tggcaaagta gttaatggtg atacgatttt tttgatccag
    10621 catcgcgccg agacggctga atgcagcagt gtcattgaca tcgagattac aaaaatccag
    10681 acgtgcgctc agggtgtccc ataaaccttc atcaatggtt tctttcatga aagtttcgag
    10741 cgcctcgcgg acaactttgg tatatgccgc tttatcccag tcagcacgcc ctacgccgat
    10801 aatccgggtg tccgggttga gctgaccggc tttttccagt tgatacaggg aaggcagcaa
    10861 tttacgacgc gcaaggtcgc ctttcgcgcc gaaaatgacc aagtcacaga cctgggctgt
    10921 ttgcgttacc gccatgtcat tctccttaag ttaactaacc cggtacttaa gccagggtat
    10981 acttgtaatt ttcttacggt gcactgtact gcttttacga gcttgcgaaa actgtaaacg
    11041 cttatccacc cgtgcgatta cggtaaaagc gcgcaaagtg cggcaaaact gataaaaaaa
    11101 tcatcgtttt ccattgggta aaaatctgac actgatcatg ttatgaaaaa aaataacaac
    11161 ttttttatct gcttttgtca ttaacacagc acacaggcgt aatatctgac aaaactgcat
    11221 ttcgatttct ttcagtgcgg aaatcgtcat tacccgtgag tctctttaca tcatgaatat
    11281 gctggaaaaa atccagtctc agctggaaca tttgagcaaa tcagagcgca aagttgccga
    11341 ggtcattctg gcttcgcccg ataacgcgat ccattcgagt attgctgctc tggcactgga
    11401 agccaatgtt agcgaaccga cggtgaatcg tttctgtcgc agcatggaca cgcgcggttt
    11461 tcctgatttt aaacttcatc tggcacagag tctggcgaat ggcactccct atgttaatcg
    11521 caacgtcaat gaagatgaca gcgttgaatc atacacaggg aaaatatttg agtccgcaat
    11581 ggcaacgctc gatcatgtcc gtcattcact ggataaatct gccatcaacc gcgccgtcga
    11641 cttgctcact caggcaaaaa aaatcgcctt tttcggatta ggctcttcag ccgccgttgc
    11701 ccacgatgcg atgaataagt tctttcgttt taatgttccg gtggtgtact ccgatgatat
    11761 tgtgctgcaa cgcatgagtt gtatgaattg tagcgacgga gacgtggtgg tgctgatttc
    11821 tcacaccgga agaacaaaaa atctggtcga gctggcgcag ctggcacgcg aaaacgacgc
    11881 tatggtgatt gccctcacct ctgcgggtac cccgctcgcc cgggaagcaa cgctggcaat
    11941 taccctcgac gtaccggaag atactgacat ttatatgccc atggtttctc gacttgcaca
    12001 gctgaccgtg atagatgtgc tggcgacagg atttactttg cgacgcggtg caaaattcag
    12061 agataacttg aagcgggtca aggaagcgct gaaggaatcg cgttttgata agcagttact
    12121 taatttaagt gacgatcgct aaaagcgact gtcactgttc taatcttata cgacatccga
    12181 atgagattat tttatcgcca tcgcggcgtt atttcattcg gatttcatgt tcaagcaaca
    12241 cctggttgtt tcagtcaacg gagtattaca tgtccagaag gcttcgcaga acaaaaatcg
    12301 ttaccacgtt aggcccagca acagatcgcg ataataatct tgaaaaagtt atcgcggcgg
    12361 gtgccaacgt tgtacgtatg aacttttctc acggctcgcc tgaagatcac aaaatgcgcg
    12421 cggataaagt tcgtgagatt gccgcaaaac tggggcgtca tgtggctatt ctgggtgacc
    12481 tccaggggcc caaaatccgt gtatccacct ttaaagaagg caaagttttc ctcaatattg
    12541 gggataaatt cctgctcgac gccaacctgg gtaaaggtga aggcgacaaa gaaaaagtcg
    12601 gtatcaacta caaaggcctg cctgctgacg tcgtgcctgg tgacatcctg ctgctggacg
    12661 atggtcgcgt ccagttaaaa gtactggaag ttcagggcat gaaagtgttc accgaagtca
    12721 ccgtcggtgg tcccctctcc aacaataaag gtatcaacaa acttggcggc ggtttgtcgg
    12781 ctgaagcgct gaccgaaaaa gacaaagcag acattaagac tgcggcgttg attggcgtag
    12841 attacctggc tgtctccttc ccacgctgtg gcgaagatct gaactatgcc cgtcgcctgg
    12901 cacgcgatgc aggatgtgat gcgaaaattg ttgccaaggt tgaacgtgcg gaagccgttt
    12961 gcagccagga tgcaatggat gacatcatcc tcgcctctga cgtggtaatg gttgcacgtg
    13021 gcgacctcgg tgtggaaatt ggcgacccgg aactggtcgg cattcagaaa gcgttgatcc
    13081 gtcgtgcgcg tcagctaaac cgagcggtaa tcacggcgac ccagatgatg gagtcaatga
    13141 ttactaaccc gatgccgacg cgtgcagaag tcatggacgt agcaaacgcc gttctggatg
    13201 gtactgacgc tgtgatgctg tctgcagaaa ctgccgctgg gcagtatccg tcagaaactg
    13261 ttgcagccat ggcgcgcgtt tgcctgggtg cggaaaaaat cccgagcatc aacgtttcta
    13321 aacaccgtct ggacgttcag ttcgacaatg tggaagaagc tattgccatg tcagcaatgt
    13381 acgcagctaa ccacctgaaa ggcgttacgg cgatcattac catgaccgaa tcgggtcgta
    13441 ccgcgctgat gacctcccgt atcagctctg gtctaccaat tttcgccatg tcgcgccatg
    13501 aacgtacgct gaacctgact gctctctatc gtggcgttac gccggtgcac tttgatagcg
    13561 ctaatgacgg cgtagcagct gccagcgaag cggttaatct gctgcgcgat aaaggttact
    13621 tgatgtctgg tgacctggtg attgtcaccc agggcgacgt gatgagtacc gtgggttcta
    13681 ctaataccac gcgtatttta acggtagagt aagtacgttg ccggatgcgg cgaaaacgcc
    13741 acatccggcc tacagttcaa tgatagttca acagatttcg aatattctga agcaaacttg
    13801 aacttatcat caggcgaagg cctctcctcg cgagaggctt ttttatttga tgggataaag
    13861 atctttgcgc ttatacggct ggatttcgcc cggtttgcga gttttcagca attttagtat
    13921 ccaggtgtat tgttctggtc gcggaccaac aaaaatctcg acttcttcat tcatccgccg
    13981 cgcaatcgta tgatcatccg cctctaacag atcatccatc ggtgggcgca cctgaatcgt
    14041 cagacgatgc gtcttgccat cataaatcgg aaacagcggt acaacgcgcg cacggcacac
    14101 tttcatcaaa cgaccaatcg cgggcaacgt cgctttatag gtggcaaaga aatccacaaa
    14161 ttcgctgtgc tctgggccat gatcctgatc gggtaaataa tatccccagt acccctgacg
    14221 taccgactgg atgaatggtt taataccgtc atttctcgca tgcagacgac cgccaaagcg
    14281 acgacgcacc gtgttccaga cataatcaaa aaccgggttg ccctgattat ggaacatcgc
    14341 tgccattttc tgcccttgcg aggccatcag catggcagga atatcgacgg cccaaccgtg
    14401 cggcactaga aagataactt tctcgttatt acgccgcatc tcttcgatga tctccagccc
    14461 ttgccagtca acgcgcggct gaattttctc cggcccgcgt attgccaact cagccatcat
    14521 tgccatcgct tgcggcgcgg tggcaaacat ctcatcaaca atcgcttcgc gttcagcttc
    14581 actacgttct ggaaagcaga gcgacagatt gattaacgca cgacggcgtg agctttttcc
    14641 cagtcgtccg gcaatacgtc ccagccgtgc cagaatggga tcacggaact ttggcggcgt
    14701 taaagcgata cccgccatcg ctgctacgcc cagccatgct ccccagtagc gcgggtggcg
    14761 aaaggattta tcaaactcag gaatgtattc gctattattt tttttcgttt ccatgctttt
    14821 ccagtttcgg ataaggcaaa aatcaatctg gtgatagtgt agcggcgcaa cttgccccgc
    14881 accaaataaa aaaagccggt actgactgcg taccggctgc gaatggatgt taattaatca
    14941 aaccgtagct gcggcacaat ctctttggcc tgcgccagga attcgcgacg atcggagccg
    15001 gtcagccctt cggtacgcgg cagttttgcc gtcagcgggt ttacggcctg ctggtttatc
    15061 catacttcat agtgcagatg cggcccggtt gaacgtccgg tattaccgga aagcgcgata
    15121 cggtcgccac gtttcacctt ctgccccggt ttcaccagaa tcttgcgcaa gtgcatataa
    15181 cgcgtggtgt agctgcgacc atgacgaata gccacataat aacctgctgc gccactgcgt
    15241 ttggcaacca ccacttcacc gtcacccact gaaagcactg gcgtaccctg cggcatggcg
    15301 aaatcaacac ctctgtgtgg tgcaacgcga ccggtcaccg gattagtacg acgcgggtta
    15361 aagttagacg agatacggaa ctgtttcgcc gtcgggaatc gcaagaatcc tttcgccaga
    15421 ccagtaccgt tacgatcgta gaatttgcca tcttcagcgc ggatagcgta ataatcttta
    15481 ccttctgaac gcaaacgtac gcccagcagc tggctttgct cacgtttacc atcaagcatt
    15541 tctcgagaca ttaataccgc aaattcatcg ccttttttca gtttgcggaa atccatttgc
    15601 cactgcatgg ctttaatcac tgcgctcact tcggcgctgg tcaaaccggc gtttctggcg
    15661 ctggcaacaa agctcccccc gacggtacct ttcagcagat tgttaaccca ctctccttgc
    15721 tgcatttcgc tggtcatttt aaaaccgtta gcggcagtac ggtcataggt tcgggtttca
    15781 cgacgagaca cttcccaggt gaggcgctgc agttcgccgt ccgcggttaa tgtccaggag
    15841 agttgttgac cgattttcag gttacgcaat tctttgtcgg ccgcagccag ttgggtgata
    15901 tcacccatat caataccata ctgattgaga atgctgctta gcgtatcgcc agtggaaaca
    15961 acatattcat gcacgcccgc ttcaccggcg attttgtcat ccagttcgtc ctggggaatg
    16021 gcttcatctt cttgtgcagc ttgatcaatc ggctcactgg cttcaggtaa gagcgaacga
    16081 atttcgttct gttccagctc aatggttttg acaattggcg tggcatcgcg gtgataaaca
    16141 tagggccgcc agacagcgac ggccagagta agaacggtga gcgaccccaa cataacgcgg
    16201 tgtggtcgcg gtaaattatt aaacgccagg gcgacagagc gggctatctg ttgcacgtaa
    16261 tcacttcctc attaatctcc tttcaggcag ctcgcatact ggttggctaa ttgactcagg
    16321 aattctgaat agcttgtttt acccagtttg atattcgtcc ccaagggatc caacgttccc
    16381 atacgaacgg atgtccctcg tgcgacgctc tcaacgaccg ctggcctgaa ctgtggctca
    16441 gcaaaaacgc aggttgcttt ttgctcaacc aactgtgttc ttatttcatg taaacgctgc
    16501 gcgccaggtt gaatctcagg gttaacggta aaatgaccaa gcggtgtcag tccgaactgt
    16561 ttttcgaaat agccgtaagc atcgtgaaaa acgaaataac ctttcccctt gagcggcgcg
    16621 agctcgttac caacctgcgt ttcggttgag gctaattgtg cctcaaaatc cttcaggttg
    16681 gcgtcaagtt tggctcgact ttgcggcata agttccacta attttccatg gattgcaacc
    16741 gctgtagccc gcgctatctc tggggaaagc caaagatgca tgttgaaatc gccgtgatgg
    16801 tgatcttcgt cacttttttc cgcgtggtcg tgatcatcat catcgccgtt aatacttttc
    16861 atcagtagcg gtttcacatc ttcaagctgc gcaatcgtta cctgcttcgc ttctggtaat
    16921 ttgcttaccg gtttttgcat aaacgcttcc atctccgggc caacccaaac gactaagtcc
    16981 gcgttctgta agcgttttac atccgatgga cgcagtgaat aatcatgttc tgaagcgccg
    17041 tcaggaagta aaacctccgt ttctgttacc ccatcagcaa tggcagaagc gatgaaccca
    17101 acgggtttaa gcgaagcgac aacggcggca tctgcggcct gtgttgcacc gccccagaga
    17161 gcggcgaata atgctgcgaa aagaagcgtt tttttatgta acataatgcg accaatcatc
    17221 gtaatgaata tgagaagtgt gatattataa catttcatga ctactgcaag actaaaatta
    17281 acatgacaag tctggtttcc ctggaaaatg tctcggtttc ttttggccaa cgccgcgtcc
    17341 tctctgatgt gtcgctggaa cttaaacctg gaaaaatttt gactttactt gggccaaatg
    17401 gcgcaggtaa gtcgacactg gtacgggtag tgctcgggct ggtaacaccc gatgaagggg
    17461 ttatcaagcg caacggaaaa ctgcgcatcg gctatgtacc acagaagctg tatctcgaca
    17521 ccacgttgcc actgaccgta aaccgttttt tacgcttacg ccctggtaca cataaagaag
    17581 atattttgcc tgcactgaaa cgtgtccagg ccgggcacct gattaacgca ccgatgcaaa
    17641 agctctctgg tggcgaaacg cagcgtgtac tgttagcgcg agcattgtta aatcgcccgc
    17701 aattattagt gctggatgaa cccactcaag gcgtggatgt aaatggccag gtggcgttat
    17761 atgaccttat tgaccaactg cgtcgtgaac tggattgtgg cgttttaatg gtttctcacg
    17821 atcttcatct ggtgatggca aaaaccgatg aagtgctgtg cctgaatcat cacatttgtt
    17881 gttccggcac accggaagtt gtttccctgc atccggagtt tatttcaatg tttggtcctc
    17941 gtggtgctga acaactgggt atctatcgcc atcatcataa tcatcgtcac gatttacagg
    18001 gacgaattgt tttgcgtcgg ggaaatgatc gctcatgatt gaattattat ttcccggttg
    18061 gttagccggg atcatgctcg cctgtgccgc gggtccgctg ggttcgtttg tagtctggcg
    18121 tcgtatgtct tatttcggtg atacgctggc tcatgcctca ttacttggtg tcgcgtttgg
    18181 tttgttgctg gacgtgaatc cattctatgc ggtgattgcc gttacgctgc tgctggcggg
    18241 cggtctggta tggctggaga agcgtccaca gctggcgatc gacacgttat tagggattat
    18301 ggcgcacagt gccctgtcgc tgggcctggt ggtcgttagt ctgatgtcta acattcgtgt
    18361 tgatttgatg gcttacctgt tcggtgattt actggcagtg acgccagaag atctcatctc
    18421 tattgcaatt ggcgtggtca tcgtggtggc tattttgttc tggcaatggc gcaatttgct
    18481 gtcgatgacg attagcccgg atctggcgtt tgttgatggt gtgaaattac agcgagtgaa
    18541 attgttgttg atgctggtga cggcattgac gattggtgta gcgatgaaat ttgtcggtgc
    18601 gttgattatt acttcgttgc tgattattcc tgcagctact gcgcgtcgct ttgcccgtac
    18661 gccggaacag atggctggtg tcgctgtttt ggtggggatg gtggcagtaa ctgacggttt
    18721 aaccttttcc gcgttttacg atacgccggc gggtccgtcg gtggtgctat gtgcggcact
    18781 gttatttatt atcagtatga tgaaaaagca ggccagctaa tctatcgctg aacacatttg
    18841 tcggatgcgg cgcgagcgcc ttatccgacc tacggttcgc tatctctggt aggcctggta
    18901 agacgcgaac agcgtcgcat caggcacact gccaatgtcg gatgcggcgc gagcgccttt
    18961 tccgacctac ggttcgctat ctctggtata tgcctgataa gacgcgaata gcgtcgcatc
    19021 aggcacactg ccaatgtcgg atgcggtgcg agcgaccaat ccgacttacg gcatttctgg
    19081 cggcgttatg ccaaagtgat tccacgcccg cgtcgtcgcc atacgcccac gcggtgtacg
    19141 ctgcaaaaag ccttgctgaa tcaaataagg ttccagcaca tcctcaatgg tttcacgttc
    19201 ttcgccaatg gctgccgcaa ggttatccag acctaccggc ccaccaaaga acttatcgat
    19261 taccgccagc aacaatttgc ggtccatata atcgaaacct tcagcatcga cattcaacat
    19321 atccagcgcc tgagcagcga tatctgccga gatggtgcca tcgtgcttca cttcggcgaa
    19381 atcacgcact cgacgcagca gacggttggc aatgcgcggc gtaccgcgag cgcgacgagc
    19441 aacttccagc gcgccgtcat cactcatctc aagccccata aagcgtgcgc tgcgactgac
    19501 gatatattgc agatccggca cctgataaaa ctccagacgt tgcacaatac caaaacggtc
    19561 gcgcaacggt gatgtcagcg aacctgcgcg cgtggttgca ccaatcaggg taaacggcgg
    19621 caaatcaatt ttaatggagc gtgccgccgg accttcacca atcatgatat ccagttggta
    19681 gtcttccatt gccgggtaca gcacttcttc aacaactggc gataaacggt ggatctcatc
    19741 aataaacagc acgtcatgcg gttcaaggtt agtgagcatc gcagccaaat cgcccgcctt
    19801 ttccagcacc ggaccagaag tcgtgcgtaa attaacgccc atttcattgg cgacaatgtt
    19861 ggcaagcgta gttttaccca accccggagg accaaaaatc aacaaatgat cgagggcatc
    19921 gccgcgcagt ttcgctgctt tgatgaaaat ctccatctgt gaacgaacct gcggctgacc
    19981 aacatactct tccagtaatt tagggcgaat ggcgcgatct gctacatctt ccggcaaagt
    20041 ggtaccggca gaaatcagac ggtctgcttc aatcatcctt tacctcataa cgcggcgcgt
    20101 agggcttcgc gaattaatgt ttcactgctg gcgtcagggc gagcgatttt gctcaccatg
    20161 cggcttgctt cttgtggttt atagcccagc gccaccagcg cggcaaccgc ttcttgttca
    20221 gcatcgtcgg tcgccgggct ggcaggagac gtgagtacca ggtcggcggc tggcgtaaag
    20281 agatcgccat gcaaaccttt aaatcggtct ttcatttcaa caatcaagcg ttcggcggtt
    20341 tttttgccaa tacccggcag tttcaccagt gcccccactt cttcacgctc aacggcatta
    20401 acgaactgct gcgctgacat tccggagagg atcgccagcg ccaacttcgg gccgacgccg
    20461 ttggttttga tcaactcttt gaacaacgtg cgctcttgtt tattgttaaa accgtacagc
    20521 agttgcgcgt cttcacgcac cacaaagtgg gtgaaaacga tcgcttcctg acccgcttca
    20581 gggagttcat aaaaacaggt catcggcata tgcacttcat agcctacgcc gcccacttca
    20641 attaacacca gcgggggttg tttttcaatg atgatgcctc tgagtctgcc tatcacatga
    20701 cgctcctgcg taatgaatca aagataatgc tgtatgataa aaaaatgctg gatagatatc
    20761 cagcgaagga tgaaaaaaac ttgcgaggtg tct
//
LOCUS       DBFIFI010000067        20785 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.067, whole genome shotgun sequence.
ACCESSION   DBFIFI010000067 DBFIFI010000000
VERSION     DBFIFI010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20785
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.067"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(483..914)
                     /locus_tag="N7B39_002367"
     CDS             complement(483..914)
                     /locus_tag="N7B39_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity TolA C-terminal
                     domain-containing protein"
                     /protein_id="HGU2422334.1"
                     /translation="MVLRSIWVEEQYMGVKKIVILAILVSFVAGCAPLHPSNCHKTTA
                     LGSCSSGRWDDQDEWGEQARGIRDAINAKLDDPQKWKRKKCRLHMEFSRDGTALKIST
                     SNGDKAYCEAIKSAAHKAKFPAFNNPEVYRDFQKSGFDMRG"
     gene            complement(982..3003)
                     /locus_tag="N7B39_002368"
                     /pseudo
     CDS             complement(982..3003)
                     /locus_tag="N7B39_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3137..3262)
                     /locus_tag="N7B39_002369"
     CDS             complement(3137..3262)
                     /locus_tag="N7B39_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HGU2422335.1"
                     /translation="MLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK"
     gene            complement(3204..3353)
                     /locus_tag="N7B39_002370"
                     /pseudo
     CDS             complement(3204..3353)
                     /locus_tag="N7B39_002370"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5431 family protein"
     gene            complement(3527..4288)
                     /locus_tag="N7B39_002371"
     CDS             complement(3527..4288)
                     /locus_tag="N7B39_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HGU2422336.1"
                     /translation="MSARSQALVPLSTEQQAAWRAVAETEKRRNQGNTLAEYPYAGAF
                     FRCLNGSRRISLSDLRFFMPSLTAEELHGNRLQWLYAIDVLIETQGEVCLLPLPGDAA
                     ERLFPSVRFCVRERSRHKSALVMQKYSRQQAREAEQKARAYQALVAQAEIELAFHSPE
                     TVGSWYARWSDRVAEHDLETLFWQWGERFPSLAGMERWQWQDMPFWQVIAEASLAAKE
                     AGHAVREMERWMVPNKLREVRDAATITRIARKQPE"
     gene            complement(4285..4719)
                     /gene="psiB"
                     /locus_tag="N7B39_002372"
     CDS             complement(4285..4719)
                     /gene="psiB"
                     /locus_tag="N7B39_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HGU2422337.1"
                     /translation="MKTELTLNVLHTMNAQEYEDIRAAGSDARRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDTQGYSVNDIIHILMAEGGQV"
     gene            complement(4774..6732)
                     /locus_tag="N7B39_002373"
     CDS             complement(4774..6732)
                     /locus_tag="N7B39_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HGU2422338.1"
                     /translation="MSVTESKAKTERKSSRKPAKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSAESVSELADSIKGVGLLQNLVVHALPGDRYGVAAGGRRLAALNMLA
                     ERDIIPADWPVRVKVIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADTFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEHLREAEGWEWCAGRMEPVGFCREDAGTYRSLPEPEAVLTEAEE
                     ERLNELMARYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDVADNDYRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQSD
                     KSLALLAWTLCLNVFGSGAYSNPARIRLECEHYSLTSDAPSGKEGAAFMAMMAEKARL
                     AALLPDGWARDMTTFLSLSQEVLLSLLSFCTACSIHGVQTREYGHTSRSPLDTLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEHHMKD
                     NRWVPGWMCAPRPQTDATERTDNLADAA"
     gene            complement(6798..7031)
                     /locus_tag="N7B39_002374"
     CDS             complement(6798..7031)
                     /locus_tag="N7B39_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HGU2422339.1"
                     /translation="MSEYFRILQGLPDGPFTRKHAEAVAAQYRNVFIENDHGEQFRLV
                     VRNNGAMVWRTWNFEDGAGYWMNHVIRDFGIIK"
     gene            complement(7088..7540)
                     /locus_tag="N7B39_002375"
     CDS             complement(7088..7540)
                     /locus_tag="N7B39_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HGU2422340.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLVRAAGAQTQPEEGQQFSGQPQPQPPEGDDYGFSDDIPF"
     gene            complement(7566..7772)
                     /locus_tag="N7B39_002376"
     CDS             complement(7566..7772)
                     /locus_tag="N7B39_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HGU2422341.1"
                     /translation="MHIPQNFLPKSRQKVLRSAPGQSFPAVAVMPLAAKVLKKVMVNT
                     GGNKRQVPAKRQNRGSRNSKDDGG"
     gene            7842..8297
                     /locus_tag="N7B39_002377"
     CDS             7842..8297
                     /locus_tag="N7B39_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2422342.1"
                     /translation="MAPDTTTGRHPPQRREARFAKRRCPLHPGGLLPGCRERRPRRDR
                     GGATGTVAASPGFASGMEARQGGDSSAGSVHDSPPRQGDARKRHKQENNKTDSNQSSR
                     KKKNSSSSSSSSSSSSSSMNNNIISNRQMTGLKRQKPTAECPRNVRGGA"
     gene            complement(8383..8946)
                     /locus_tag="N7B39_002378"
     CDS             complement(8383..8946)
                     /locus_tag="N7B39_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HGU2422343.1"
                     /translation="MHSQLKERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVT
                     LAEISNRDHILEPSAGTGAILRAIRDTAPEAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWQPVQYYSRVIINPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREELPRGTFAYTDVPTMIIRLRA"
     gene            complement(8993..10354)
                     /locus_tag="N7B39_002379"
     CDS             complement(8993..10354)
                     /locus_tag="N7B39_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HGU2422344.1"
                     /translation="MTSAAITMTAPEAASPVQMYRATYSPDDNKLRLYAVSRLDPETY
                     KKVHDAGFRWAPKQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERGRSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLNYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVAEAEDHGAYRYRRTMDNNFRLVNVYITDMKITEI
                     PQK"
     gene            complement(10406..10636)
                     /locus_tag="N7B39_002380"
     CDS             complement(10406..10636)
                     /locus_tag="N7B39_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2422345.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPAPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            11153..11662
                     /locus_tag="N7B39_002381"
     CDS             11153..11662
                     /locus_tag="N7B39_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001358895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2422346.1"
                     /translation="MSRDPPQRREARFAKRRCPLHPGGLLPGCRERRPRRDRGCATGT
                     VAASQGFASGMEARQGGDSFAGSVHDSPPRQGDARKSHKQERNGKKRRKRKRKKKKNS
                     NRHGNHRKITSEKNIPDTSQTGSVTDDASAWPASLPAGVSLPGVFARADRRPPPPALY
                     HLFLLPCKM"
     gene            complement(11632..11823)
                     /locus_tag="N7B39_002382"
     CDS             complement(11632..11823)
                     /locus_tag="N7B39_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2422347.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            complement(11820..12242)
                     /locus_tag="N7B39_002383"
     CDS             complement(11820..12242)
                     /locus_tag="N7B39_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HGU2422348.1"
                     /translation="MYCTVKEIIRDVLDTDVPDSECVFAVVLTRGDVRHIAQDWNLSD
                     DELETVMQRLDDAFEYGADVSIVHDVVRELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            complement(12289..12714)
                     /locus_tag="N7B39_002384"
     CDS             complement(12289..12714)
                     /locus_tag="N7B39_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HGU2422349.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADACGIILTSLVINRQLW
                     LYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            complement(13132..13908)
                     /locus_tag="N7B39_002385"
     CDS             complement(13132..13908)
                     /locus_tag="N7B39_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2422350.1"
                     /translation="MNENTTLNALICRHARNLLLAQGWPEETDVDQRNPKYPGWISIY
                     VLLDAPRLATLLVNRHGGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQV
                     SFPYAGEWLTEDEIRAVLAAVRDAVRSVSCRVAEDTRRIRAALTTTGQTLLTRQTRRF
                     RLVVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDV
                     LRIPDEPPRRWFDRGVLREVVREARNEIRSMADALAKIRK"
     gene            complement(13954..14508)
                     /locus_tag="N7B39_002386"
     CDS             complement(13954..14508)
                     /locus_tag="N7B39_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HGU2422351.1"
                     /translation="MCLLNAWHARRCIAPIACSWRRIPKFWNWMRALKIEKETRMYGT
                     CETLCRELAAKYPGYTPLMLVIWSPEEIQALADGMDISLSDPEIRTVLARLEDIPEDQ
                     RIESGISSAAAMEIISNVSENRQVTVPAELLASLIQTAEQALWKREWAARDYGLAVPE
                     CVTRRQAVVNQARILLKNNTHENG"
     gene            complement(14402..14623)
                     /locus_tag="N7B39_002387"
     CDS             complement(14402..14623)
                     /locus_tag="N7B39_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2422352.1"
                     /translation="MNYAGHEKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVER
                     LARQTLYRANRLFMEAYTEILELDACFKD"
     gene            complement(14624..15307)
                     /locus_tag="N7B39_002388"
     CDS             complement(14624..15307)
                     /locus_tag="N7B39_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HGU2422353.1"
                     /translation="MSRFIQGDCVRVMATIPGNGVDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKTYT
                     SKAAYVGYRHECAYILAKGRPALPQKPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(15384..15689)
                     /locus_tag="N7B39_002389"
     CDS             complement(15384..15689)
                     /locus_tag="N7B39_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2422354.1"
                     /translation="MTETGGQPPVSFPVKDVAGLLFLLRRLTRRGRNAACGQCPASGR
                     ARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGIFSPSKRRRLRYSTVGLTRYRTR"
     gene            complement(15693..16595)
                     /locus_tag="N7B39_002390"
     CDS             complement(15693..16595)
                     /locus_tag="N7B39_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HGU2422355.1"
                     /translation="MYFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QTTEDVRFEPCPGLTAAGRGVVSPENIAFTRWLTHLQDGVLLDEQNCLMLHELWLQSG
                     TGRRRWEELPDDARESITALFTPKRGDWCDIWSNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLSTRLDVEVNGFNGGVLNGVPSAYHWYTEQYGVKWPCGYEVNISSQGDNFIQVDF
                     DTPWCQPESDVIAVLSRRFSCMLEHWYAGQGCNFCGWQRYERGELVDVLWGELEWSSP
                     TDDDELPEVTAPEWIVDKVAHYGG"
     gene            complement(17290..18261)
                     /locus_tag="N7B39_002391"
     CDS             complement(17290..18261)
                     /locus_tag="N7B39_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family plasmid partition
                     protein"
                     /protein_id="HGU2422356.1"
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGN
                     AITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQ
                     TPAFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSA
                     YERGQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSA
                     RSGDALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLS
                     SRHQFAPGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     gene            complement(18261..19427)
                     /gene="sopA"
                     /locus_tag="N7B39_002392"
     CDS             complement(18261..19427)
                     /gene="sopA"
                     /locus_tag="N7B39_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid-partitioning protein SopA"
                     /protein_id="HGU2422357.1"
                     /translation="MKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRIGE
                     AADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRAE
                     DVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPT
                     DPHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQ
                     FFDMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRE
                     TDEVGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     gene            complement(20015..20770)
                     /gene="repE"
                     /locus_tag="N7B39_002393"
     CDS             complement(20015..20770)
                     /gene="repE"
                     /locus_tag="N7B39_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepE"
                     /protein_id="HGU2422358.1"
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVD
                     QIRKSDGTLQEHDGICEIHVVKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEED
                     AGDEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNP
                     YAMRLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINS
                     RTPMRLSYIEKKKGRQTTHIVFSFRDITSMTTG"
BASE COUNT         4747 a         6075 c         5231 g         4732 t
ORIGIN      
        1 tttgttctct tttaaaatat attccacgag cgtcttgtgg gaccttttgc tgttataggg
       61 gggcagtttg gatatcgaaa attattgtac gttaaggctg aatttcaatc gcaaagcata
      121 accttacctt tctgcttact tatgcagttc aaagagtacc aactctgcaa ttttcgttgc
      181 cacagatgca atatcgttac tttccagtca ccatgccgct gaaagcccac accatgcgat
      241 aatccacatc aagagccgct ttctgtccag acccgctgtt gtgaggtgtg aggtgtgagg
      301 tgtgaggtgt gaggtgtgag gtagcctgag tttaacggac aggccgatga aggacgccgt
      361 taccgggata atgtaagaat ctttttttga gtatgtcaac ataacctgag gcaaagtacc
      421 tcaggttcca gtaatcagcc gacctgacag tcttctgtta gccctacaga tatgtcgtgt
      481 agctaaccgc gcatgtcaaa gccggatttt tgaaaatctc tataaacttc cggattgttg
      541 aaggccggga atttagcttt atgggctgct gactttatcg cttcgcaata ggctttatcg
      601 ccattactgg ttgatatttt taacgccgtg ccatcccggg agaattccat atgcaacctg
      661 cattttttcc ttttccattt ttgcggatca tcaagtttag cattaattgc atccctgatt
      721 ccccgtgcct gctcccccca ttcatcctga tcatcccaac gtcctgaact gcaactaccc
      781 agggctgtag ttttatggca atttgaagga tgtaatggcg cacatcccgc cacaaagctg
      841 actaaaatag ccagtataac gattttcttt acccccatat attgctcctc aacccatata
      901 gatctcagta ccatagctct gtcagagcaa agaaaatctt aactaataca agttaagatt
      961 tttaatatcc actcctcatc cctacattcc aggtgatggt ggcgtccact cattatcctg
     1021 aactggagca taagatgcgc catgcccccc ggatatgctt ttctgttgcc tgatatattc
     1081 gtggtagtac tgttcactac gctgacgatg taaatccaca tccgggcggc tgattacaaa
     1141 gtgtccattt ccggttattc cagctgcatt atgccaggcc accatttccg caaatacatc
     1201 attaaactgt tgatatgagc cttccggttc aaaacccatt cctggctcac gatcaaacaa
     1261 tacaggagca atcagattca gcttatccac cagttcattt ctgtcctgat gatggtagaa
     1321 caccacacgg tccccataat ccacacagac taactcgcca ttcctccgat cacgaaaacg
     1381 cacactgcca tctttctgca gacgggcttc gagaacgcca gtgtcttcat aaagtggagt
     1441 tcctcccggt tcacagagaa tcacgcagcg gtcggcattc gttgttcgtc gcttgtcttt
     1501 gcgacgatca cgataatccc agccacgtaa ctgtgataat gcagctctgt ctccctgtac
     1561 cgcctgttgc tccacccact gccggtagga caaaggatac cacttaccct cctcaatcag
     1621 tgacaaccgc tcttccttca cactctcctt cagcctgatc agcgcctgca tacgctgcac
     1681 ttccgcaata tggtaatgca gcttacgcaa ttgtggatcc cggaactgca cacggatata
     1741 cgccttgcgc cgacggcagg cagcatgaat ttcccgtaac cgttctccgt aacgtaagtc
     1801 gggtttacgc cagtgctcct tccatgcaag ataacgcgcg cgcaaatctt cccgcgcagc
     1861 tgcacgggct tcacggcgtt cacgacgcag tacaggatcg cgccggaagc cgggctccac
     1921 ttcatcgctg gcagcaagtt ccggattgta gcgacattca ggcttcaccc gttcaaatat
     1981 atctgcacct gcaatctcaa atggccctgc ctgcggctca gccctggata acgtcaaatc
     2041 cgggtggata ctgctggcct tcactggtgt gagctcatga ttaaacgcat ccacaatcac
     2101 aagaccatga tgctgtttct gcaacataag ccctttacgc gcaaacatct catggcacgc
     2161 ctgccagctg aaattcccgc ccgcgatcat atcttcaatc aacgactcac actgaacagt
     2221 tgtgtacccc cacagacttt ccttatcaga aaacagttcg cgctttattg ctccctgtgg
     2281 acctgtcccc atccgcatac gataccattc gtaatcactg acagcctgag taccaagatt
     2341 acgtccccgg aaccgttctc cgggtataac ctgagtaaaa atatccttcg gtacaggctg
     2401 ccatccacca tccagatcct gcatataatc cagcgtcatt aacggccaca cactgtctgc
     2461 acgtactggt gtctgttgtt tatcaaatcc atcatgcagt accagatgcc cgtgctggat
     2521 ccccatccat aatccagccg ttgccagagt tctgtgcaca tccaggcaac tttccagctc
     2581 accatttctc gccatttccg gtaagcgttc ggcaaaatga cggcaggcgt actgtttcag
     2641 actctccgtt tctgccactt tttccggccg gatatcaacg tttatggctt ccggatcata
     2701 acgaccgggt gtccccacct gactgaaaat atctgtcggt acaggctgat attcccccag
     2761 tttccgcccc agtttttcag ctgtccataa cggtccgaac gaactgagtg caacaccttc
     2821 ccgcgctctg tcccagccat ccttcaccac cagttcccct tcctgcatcg tgatatacag
     2881 accatcactg gccagccgtt tatgcaaaga taaccagtcc tgtgcgggct cttcacgcag
     2941 cccggcaatc gacatctgag caatatattc cctgaacgtc tgctttttcc cgcgtcgcca
     3001 ggctgttcat gcccgtacaa tgccagcaag gagaaagggg ccaccggcga accagcagcc
     3061 cctttataaa ggcgcttcag tgcagtcaga ccagcatcag ccctgaaaag gcgggcctgc
     3121 gcccgcctcc aggttgctac ttaccggatt cgtaagccat gaaagccgcc acctccctgt
     3181 atccgtctct gtaacgaatc tcgcacagcg attttcgtgt cagataagtg aatatcaaca
     3241 gtgtgagaca cactatcaac acacaccaga caagagagct gcgtggtagt ttcatggcct
     3301 tcttctcctt gcgcaaagcg cggtaagagg ctatcctgat gttgtctaag catgcagggg
     3361 cctcgtgggt taatgaaaaa ttaactacgg ggcttttgtc cttctgccac acaacacggt
     3421 aacaaaccac cttcacgtca tgaggcagaa agcctcaagc gccgggcaca tcatagccca
     3481 tataccagca cgctgaccac attcactttt cctgaaaata acccgctcat tcaggctgtt
     3541 tacgggcaat ccgtgtgatt gttgccgcat cacgcacctc ccggagtttg ttcggcacca
     3601 tccaccgctc catctcacgg acagcatgac cggcctcctt tgctgccaga ctggcttccg
     3661 cgataacctg ccagaatggc atatcctgcc actgccagcg ttccattccg gccagtgacg
     3721 gaaaacgctc gccccactgc cagaacaggg tttccaggtc atgctctgcc acccgatcac
     3781 tccagcgtgc ataccagctt ccgactgttt ccggtgaatg gaacgccagt tcgatttcag
     3841 cctgggcgac cagcgcctgg tacgcacggg ctttctgctc tgcctccctc gcctgctggc
     3901 ggctgtactt ctgcatgacc agcgcggatt tatggcgact gcgttccctg acacaaaacc
     3961 gcaccgacgg gaacaagcgt tctgctgcat caccgggaag gggaaggagg cagacctcgc
     4021 cctgtgtttc aatcagcaca tcgatggcat acaaccactg aagacggttt ccgtgcagtt
     4081 cttctgcggt cagggacggc atgaaaaatc gcaggtcaga cagcgatatc ctgcggcttc
     4141 cgttcagaca gcggaaaaac gcccccgcat acggatattc cgccagcgtg tttccctgat
     4201 tgcggcgttt ttccgtctct gccactgccc gccatgcggc ctgttgctct gtgctgaggg
     4261 gaaccagtgc ctgtgaacgt gcgctcatac ctgacctcct tccgccatca ggatatggat
     4321 gatgtcgtta acgctgtatc cctgcgtatc cagtgacgcc gccagtgcca gtgaatggct
     4381 gacggcttcc ggtgcaaacc gccgctgtac ctgaaccacc gcaaacggct caccaccggc
     4441 attcaccaga accagcaccc agacctggga gacatcgccc ggtgaacata acgccaggtg
     4501 aaaacgttcg tgggcaggcg tgaaacggac ctggacgggg aaaaagccgc caaactcgct
     4561 gccgtactcg ccgttcatcg tccagttgtc cggcgcatcc agctcccgca tcacggcgtg
     4621 tgtcagctca cggcgtgcat cacttccggc agcccggata tcttcatatt cctgtgcgtt
     4681 catggtatgt aatacattca gagtcagttc agttttcatc acggtatctc ccttgctgcc
     4741 gccccgtctc cggcggggcg gtgtggttgt tcatcaggcg gcatcagcca ggttatcggt
     4801 gcgttcagtg gcatccgtct gtgggcgtgg tgcacacatc cagccaggca cccagcggtt
     4861 gtctttcata tggtgctctg catgttcagc cgcatcgcct ttcttcatct tctccgcgtc
     4921 ccgtgcggca ccggatagtc ctgcctcatt cagggctgcg ataatctgcg gctttttcag
     4981 gtgtccgaaa aagtttgctt tcgtcggctg ccaccagtcg cgcatgtgaa agccgatggc
     5041 gctctccagc gtgtccagcg gactgcgtga cgtgtgacca tactcacggg tctggacacc
     5101 gtggatactg cacgcggtgc agaaactgag cagtgataac agcacctcct gactgagtga
     5161 caggaacgtc gtcatatccc gtgcccatcc atccggcagc agggcggcaa gacgggcttt
     5221 ttctgccatc atcgccatga atgcggcacc ttccttccct gacggcgcat cgctggtcag
     5281 cgaataatgc tcacattcca ggcggattct ggcgggattg ctgtacgctc cactgccaaa
     5341 tacattcaga cagagtgtcc atgccagaag tgccagggat ttgtctgatt gctgcatcag
     5401 tgccgcctgg actgccagcg tgcgctctga ggacattttc gtcagcagcg gcagactgat
     5461 ttcctccact gaggctttct cctgcacctg ttccgtgcgg taatcgttat cagccacatc
     5521 atcctcactg cgcaactgca caccacgctg gacacacacg ttgccataac gccaggacac
     5581 caccacgcca cttccggcac gcatctccgg cgtccacgcc ctgaccttcg ccatgcagcg
     5641 catcagcttc atttcggctt ccagcaggtc ggattcctca cactggtttt ccagcgcatc
     5701 gtaacgtgcc atcagttcgt tcaggcgttc ttcttctgcc tccgtcagca ccgcttccgg
     5761 ctccggcaga ctgcggtatg ttccggcatc ctcacggcag aaaccaacag gctccatacg
     5821 tccggcgcac cattcccagc cttcggcttc ccgaaggtgt tcggcgacag cctggagttt
     5881 ttccagcagg gcggcatcga gcgccacgca gtccacatag ccaccctcgt cgtcactgaa
     5941 caaatcggtg cgcagttcat ctggcgagaa ggtatcagcc cccacgaagc ggaatttact
     6001 gttccctgcc accgccactt cactttcggt caccagacga cgaatggtct gtacttccgg
     6061 tttaccgccc catcccgact gacaggcggc ttcaaacacc tgcacctgac gcgcggtgtc
     6121 gttctccagc gccagcgcct gacagtgctc ggtggtgatg cggtcttctg ccagcgcatc
     6181 gaggatgaca ggcgcaaggt ctgccagttt cagcattcgc tgaacgtggc ggggcgaata
     6241 acccagcaaa tcaccgattt gtgcaggtgt tttgccttcc tgcgccattg cacggaatcc
     6301 ggcaatctgt tcggcagggt gcatatcccg acgatgaccg ttctcggtca tcgatgcggc
     6361 agtcgccagc tcctgcggaa tgaccttcac acggacaggc cagtcagccg gaatgatgtc
     6421 acgctctgcc agcatgttga gtgctgccag tcggcgacca cctgcggcga caccgtaacg
     6481 gtcacctggc agggcatgaa caaccagatt ctgtaacagg ccgacacctt taatggagtc
     6541 cgccagttcg ctgacggact ccgcagaata cggcaccgtg cgcacattca gcggtgactt
     6601 aatcagcgag gccagcggca cgctcacttc ctccgtctgc gccagcaggg ccgacaggac
     6661 tgtttcctgc gtttttgcag gtttacggct ggatttacgc tccgtttttg ccttagactc
     6721 tgtaactgac atagttagta cctcccaaaa cggtaataat gattgtctgc cggacgcgtt
     6781 cccgtccggc acctctctta cttaataatc ccgaaatcac ggatgacatg gttcatccag
     6841 tacccggcac cgtcctcaaa attccaggta cgccagacca ttgcaccgtt attacggaca
     6901 accaggcgaa actgttcgcc gtggtcattc tcgataaaga cgttccggta ctgtgcggca
     6961 acggcttcgg catgtttgcg ggtaaaagga ccgtcaggca gtccctgaag tattctgaaa
     7021 tattcgctca tcctcatctc tccggtgatg ccccgcacag gacggggcgg ttgtcacagt
     7081 cagtcgttca gaacgggata tcgtctgaaa acccgtaatc gtcaccctcc ggcggttgcg
     7141 gctgaggctg accgctgaac tgttgcccct cttccggctg agtctgagca cctgcggcac
     7201 gtaccagcat ctgcatggtg cccgtggtct taacaagaat ttcggtgacg taacgggtga
     7261 taccgttatc ctcccagcta cgggtgcgaa gctgaccttc gatgtagacc tgcgcgccct
     7321 tgcgcagata ttcacctgcc acttccgcga gcttgccgaa cagcaccacg cgatgccatt
     7381 ctgtctgctc ccgcatctcc cccgtctgtt tgtcacgcca gctttctgac gtggccacct
     7441 gcaggtttgc cactgcgccc ccgttgggga tgtaacggac ttccggatct tttcccaggc
     7501 gtcctacgag gatgaccttg ttaatgccac gaactgccat gatgtgtctc cttctgttga
     7561 tgttttcagc ccccatcatc ttttgagttt cgggagccgc ggttttgacg ttttgcgggg
     7621 acctgccttt tgttccctcc ggtattcacc atcacctttt tgagaacctt cgccgcaagg
     7681 ggcatcactg ccacggccgg aaacgactgt ccgggggcag accgcaaaac tttttgcctg
     7741 cttttgggca aaaagttttg cgggatgtgc atttcacccc tggactggcg gagacggttg
     7801 tggctgtctt tgcccccggc gacaggggct taaacaggtg aatggcaccg gacacaacaa
     7861 cgggcaggca cccgccgcaa cgacgtgaag cgcggtttgc gaaacggcgt tgccccttac
     7921 atcccggcgg ccttttgccg ggatgccggg agcgcaggcc acggcgcgac agaggcggcg
     7981 cgaccggaac ggtcgctgca agcccgggtt tcgcgtcagg gatggaagcc cgccagggcg
     8041 gagacagctc cgctggctcc gttcacgaca gcccgccccg ccagggggac gcccggaaaa
     8101 ggcataagca ggagaacaac aaaacagaca gtaatcagag cagcagaaaa aagaagaaca
     8161 gtagcagtag cagtagcagt agcagtagca gtagcagtag catgaacaac aacatcatca
     8221 gtaacagaca aatgacaggt ctgaaaagac agaagcccac agcagaatgt ccgcgtaatg
     8281 taaggggcgg cgcgtgagcg cggccccttt cagcgccacc ggcgcgcggt cttttcccgg
     8341 cgcgggcaga cgggcattta tcctcccgcc tgcccggagt catcaggcac gcagacgaat
     8401 aatcatcgtc ggaacatcgg tataagcaaa cgtgccgcgt ggcagctcct cgcggacgtc
     8461 agaaaacggt aacagcttct cctgctggcg cggcccgttc agacagacgg cgaccagcac
     8521 gccacccgga cgcaacaggg aaaaggcccg caggatatgc cggatatcct gcccgtggct
     8581 gaacggcgga ttaatgataa cccggctgta atactgcacc ggctgccatt ccatgaagtc
     8641 gccacactga accctgacac cgttaaaatt ttcccgcaga taccggacca gcccgctgtt
     8701 gatttccacc gcatcacaca tggcctccgg tgcggtatcc cgaatcgccc gcagtatggc
     8761 cccggtgccg gcagagggtt ccagaatgtg atcacggttg ctgatctccg ccagcgtcac
     8821 caggcgatca cagaccgggg caggagtcac aaaaagctga gattcagccc ggatttcagc
     8881 aaccggcgcg gcgttatcca gccttgcgcg catcagccgg atacgttctt ttaactgact
     8941 gtgcatgtca ttatctccgt catcccccgg ggatttcccg gggggatata ccttatttct
     9001 gtgggatttc cgtaattttc atgtcggtga tatacacatt caccagacgg aaattattgt
     9061 ccattgtgcg gcggtagcgg tatgccccgt ggtcttctgc ttctgccaca ctgcgcacgg
     9121 ccttacagtc ccggggcagg gcggcccact gtgcctttgt catttcccgg aaaccttccc
     9181 ccggatagtt gactaccggc ggacgcttcg cggcctggct ggcgacggca gcctcttctg
     9241 ccgatggtgc tttgtagtcc gtgatgcgat cgggcgtcac tttcatcgtg ccgctgtacc
     9301 cgagaaaact gtaattcggc gttgtgactg aactcaccgc cccgttgctt ttgttcacgc
     9361 ggatgatggt cagccactcg ccccggctga aaacctgtcc gcccggctca aaatcctgtg
     9421 tccgggtaac cacgccgccg ctttcgtcca gcatggcacg ctcatagttc aggcggttct
     9481 gatagtggtt aacccagcgt tgctgatgct gaatctgccg ttcatgacag cggatcgcaa
     9541 tttcgcgggc ctgctccgtg gtgatgatgt catcatccag ggccgaccat aacgaccggc
     9601 tcccttcata ctggcttttt tctgccgggc gcggatacgt atccagcggg aaacaggcgc
     9661 tgatatggtc atgactgctg atgagttttg ccatattcag atccagcgat tcagcccgcc
     9721 acatcgtcag atatttctgc gactgcgcga tggtcttttc agccttgcgc aaatcagctt
     9781 cgattttttt gatacgacgc cagcgaacgt ccggacgttc tttatacttc gcgtgaagca
     9841 gtgctgaccg cccccgttct tcccagtatt ccgcacgttc aaagagcatc acggcacgtt
     9901 tcatgccgtt ttcaatacgc tgcgcatcac gacgggcgcg gcgttcgctg tgatgcccca
     9961 caagaatggg ctgaccgggc gggatcatcg cggccagtct ttccacttca tcaagtgcct
    10021 gtgcggattc actggcccgc tttccgctgt atccggtaaa ccgctccgcc cgtgcttcct
    10081 gacgttcagc gagcgtgctg tcttcatcct caatctctcc ggcaagtgag aggagcacgt
    10141 cttcccggcc cggtgtccag gccggcgcga caaacagcgc ctgttttggt gcccagcgaa
    10201 aaccggcatc atgcactttt ttatacgtct ccgggtcaag acgtgacacg gcatacaggc
    10261 gcagtttgtt gtcatccggt gagtaggtcg cgcggtacat ctgcacaggg ctggcggctt
    10321 ccggggcggt catggtgatt gctgctgatg tcataaaatg ttctccttcc gtctgttgcc
    10381 gcccctcgcg gggcggctca cctcatcagt taaaagcgcg tttcagttcg agtgtgcgga
    10441 gcacctgccc gaaagccgtg tttttcatgg cggggatgtg tacaccacgc ccccggtgaa
    10501 aactccgggc tatgtgtcgc ccgaaggctt tcagcacggg gtcaatttcc ggagcgggga
    10561 tgtcttcgcg tctgaaacgg ggatgacggc ggggctttac agccgctgcg gaaggtgaaa
    10621 caatgcttac agtcataaat ggattcctta tgacaaattg gttaacaatg ccgtcaaaat
    10681 tggtcgcaca attcttacgg cgtggattaa gcaggtccct gacctgcttt tttcatatct
    10741 gcatctgacg ctttccggtg ttttcctcct tctgttcctg cgttctcatt acctgtcagc
    10801 cggatgactg acagaacaca taaatgcgta ttgccggagc ctgaaataaa tcctgatttt
    10861 taaagaacat cacccgttga gtttcgggag ccgcggtttt gacgtttcgc gggaacctgc
    10921 tgctgtattc cctgtggtat gaacttcacc cgtttgagaa ccttcgccgc aaggggcatc
    10981 actgccacgg cctgacacac ctgtcctggg cagaacgcaa aattttttac cgggttctgg
    11041 gtaaaaaatt ttgtgggctg ttcatttacc ctggacagtc agtgcggcag gttgtggctg
    11101 tatcagctcc cggcgacagg gactcatacg ggtgtgtggc acccggcagg caatgagcag
    11161 ggacccgccg caacgacgtg aagcgcggtt tgcgaaacgg cgttgcccct tacatcccgg
    11221 cggccttttg ccgggatgcc gggagcgcag gccacggcgc gacagaggct gcgcgaccgg
    11281 aacggtcgct gcaagccagg gtttcgcgtc agggatggaa gcccgccagg gcggagacag
    11341 cttcgctggc tccgttcacg acagcccgcc ccgccagggg gacgcccgga aaagccataa
    11401 gcaggagagg aacggaaaga agaggaggaa gaggaagagg aaaaagaaga agaacagtaa
    11461 caggcacggt aaccacagaa aaataacatc agaaaaaaac attccggaca catcacaaac
    11521 aggaagtgta acggacgacg cgtcagcgtg gcccgcttcc cttccggcag gcgtcagcct
    11581 gccaggggtt ttcgcgcggg ctgacaggcg gcctccaccg ccagccctgt atcacctgtt
    11641 tctgctgccc tgtaagatgt agtgaccgcc caggaaaaca gcctgctgat tcgccacaaa
    11701 atcgcagatt tcatcaacaa tctgcgcggt ggtgagcggt ttctgcccca tttcacgacg
    11761 tcgcgcatta atcacggctt tgtaattgcg cagaatttcg cgggtttctg tgctgatatt
    11821 catgacatgt gctccccgtc cagcgcctgt aacacaacgt ccattgcttc gcgttcctcc
    11881 ctgacgaacg catcaccgtc gcccccgttc tcactcccga cggcatacag gcgcttcatt
    11941 tcactgcctg ccagcgccat cactttttcc agcatcaccg ccggaactgt cacctgacgg
    12001 ctggcacgct tttcttccat caactcacga acaacgtcgt gaacaatgct gacatccgca
    12061 ccatattcaa aggcatcgtc cagccgctgc atgacggttt ccagctcatc gtctgacagg
    12121 ttccagtcct gggctatgtg gcgcacatcc ccacgggtca gcaccacggc aaaaacgcat
    12181 tcactgtccg gcacgtctgt atccagtaca tcgcggatga tttctttaac agtacagtac
    12241 atgttctcgt ctcctttaca ggggcggcat tgccgccccg tccgttaatc agtccagtgc
    12301 cgcgtaaatc gcgttgcatt cagggtgaaa ttcgatgtga cgccacaact gcgcatcgcg
    12361 catccggtaa agctgggtca gtcctgcatc accgctgtcg tggtacagcc acaactggcg
    12421 gttaatcaca agggaagtca gaatgatgcc gcaggcatca gcactcacgg tacggtcaaa
    12481 ccagtttgca ccgttcacca tgtggaaacg gtcgccatcc ggcatcatgt aaccgccacc
    12541 gtccggcagg cggatgaagt gccagaagcc tccctgatat tccggcatca ttttttgcgc
    12601 cagcgcgtac acatacgcct cggcatacag aaaatcgttg ccaaacaggt aaggcagaaa
    12661 ggagagacgg tcgcactctt caacattcag agtgacaggt ttcgcatatt gcatgatgtt
    12721 ttcctccgtt gttcacatat tccgcgacac cgggcggtgc cgtgcctcca gaaaagccgg
    12781 gcgataaggc ggggatgcac agggcgcaag ggaagccgca gcctgacgca cacggctgac
    12841 gggagttgca gggtgaggcc gcgaaacctg agcgggcgaa cgaccggaaa atcacggcag
    12901 acgggaagga gggaccgggt gaccgcagcg gcgaagcgta cccttgcatc aacgccagcc
    12961 cggctaagat ggaggcacgg caccgcccgc acacgacgct gaggccacat aactgaaaaa
    13021 ccacaaggcc atgccctgca tggccgggta aagcggagcc agcggcgact tcctttgtgc
    13081 tctgtccggg gaccggcagg aaaaaggcgg ggatttaccc cgccaacgtc atcatttccg
    13141 gatttttgcc agggcatccg ccatgctgcg gatttcgttc cgggcttccc ggaccacctc
    13201 ccgcagaaca ccacggtcaa accaccggcg gggcggttca tccggtatac gcagcacatc
    13261 gcaggccagc ccactggaca ggatatgttc aatacaatcg ctgaccagat acatttccac
    13321 cgccgaaaaa cgtgcgcccc ggttcaggat ggcatcgagc accacgggca ggttttcgtc
    13381 gtcctcatcg agccagcagg ggtgatcgct ttccttcacg accaggcgaa agcggcgcgt
    13441 ctggcgggtc agtaacgtct gaccggtggt ggtcagcgcc gcccggatac gccgcgtatc
    13501 ttctgccacc cggcaactga cactgcgtac cgcatcgcgc accgccgcaa gtacagccct
    13561 gatttcgtct tcggtcagcc attcaccggc atacggaaaa gacacctgcg ttccgtctgc
    13621 cggaagcacc ggcagcgact gccactgact gccggacaga acaagttccg ctccggttcc
    13681 ggtcagtttc tgaatggcgg aggccaggtg cggcggcagt acgccgccgt ggcggttaac
    13741 gagtaacgtc gccagccggg gcgcatccag cagaacataa atgctgatcc agcccggata
    13801 cttcgggtta cgctggtcga catccgtctc ttccggccag ccctgcgcca gcagcaggtt
    13861 acgggcgtga cgacaaatca gtgcgttcag cgttgtgttt tcgttcatga ttaatcgttc
    13921 tccctgtatc gccgcccgca ggcggcgata acatcagccg ttttcgtgtg tgttgttttt
    13981 cagcaggatg cgggcctgat taaccaccgc ctgacggcgg gtaacgcatt ccgggacggc
    14041 gagaccgtaa tcacgggcgg cccattcacg tttccacagt gcctgttcgg cggtctgaat
    14101 cagggacgcc agcagttcag ccgggacggt cacctggcgg ttttcgctca cgttgctgat
    14161 aatctccatc gccgcggcag aagaaatacc ggactcaatt cgctggtctt ccgggatgtc
    14221 ctccaggcgc gccaggacgg tcctgatttc aggatcggac agggaaatat ccattccgtc
    14281 agcgagggcc tgaatctctt ccggggacca gataaccagc atcagcggtg tatatcccgg
    14341 atactttgct gccagctccc ggcatagcgt ttcgcatgtt ccgtacattc tcgtctcctt
    14401 ctcaatcttt aaagcacgca tccagttcca gaatttcggt atacgcctcc atgaacaggc
    14461 gattggcgcg atacagcgtc tggcgtgcca ggcgttcaac aagcacatcg gaatcaaagc
    14521 gacagcgatg ctccatatcg ttcagcctgg cccgaaggtc acacatggtg ttggccacct
    14581 ccgccacgtc ggcgcgaagt ttttcatgtc cggcatagtt catttacgca gcctccggca
    14641 taaaccagtc atcatttgcg gccccctgct gcatggcccg ctgtacggca gccagacgtt
    14701 gctgtccggc acggtgatac tgttcaagca gctcgatacc gatataccgg cgtccggact
    14761 gaagggcggc cacacaggtt gagccgctgc ctgcaaacgg gtccagcaca atcgcgttcg
    14821 ggtgtgtgaa gctctcaatc agtggttgca ggctggtaac aggcttttcc gtcgggtgat
    14881 gacgattgcc tgaatatttc cagcccagca cgtcgggcaa tggcttctga ggcagcgccg
    14941 gacgcccttt tgccaggatg taagcgcatt cgtggcgata gcccacatat gcggccttcg
    15001 aagtgtaggt tttggtaaaa accaggtggc caaccacgct gaatcccgca tttttccagg
    15061 cggccataaa gcgatcgacg cggttccagc catagaagct caccatcagc gcatcttttt
    15121 tcagcacgcg gtacatttca ttacaggccg gttgcagcca ttcatcggtt ttatcgccgg
    15181 cgatggtacg cccctgacgg tcacggaaac cgacgagata tggcgggtcg gtgaggatga
    15241 aatcgacacc attgcccgga atcgtggcca taacacgcac gcagtcaccc tggataaaac
    15301 gggacataac agcactcctt ctcttacaga gggcaccggg agggcgcccc tgtggcgccc
    15361 gttccccgcg gcctgtgggc tgttcaccgc gtccggtaac gggtcaggcc gaccgtggaa
    15421 tatcgcagcc gtcgtctttt tgacggcgag aatatgccgc gaaagcggct tgaagggcgt
    15481 tcatcagaaa agggcgacgg gctggcgtac actgtcaccg atgccagccg ggagcggtaa
    15541 aagcgcccgt cccgtgcccg gccggacgcg ggacactgac cacaggcagc gttcctgccg
    15601 cgccgcgtca gccggcgcag taaaaagagg agcccggcga catcctttac cggaaaagaa
    15661 acgggcggtt gcccgcccgt ctccgtcata cttcatccac cgtaatgcgc caccttgtcg
    15721 actatccact ccggcgcggt gacttccggc agctcatcat catctgtcgg ggaagaccat
    15781 tccagttccc cccacagcac atcaacaagc tctccacgct catatcgctg ccagccacag
    15841 aaattgcagc cctgcccggc gtaccagtgc tccagcatgc aactgaaacg gcggcttaat
    15901 actgcaataa cgtcgctttc cggctgacac cacggcgtgt cgaaatcaac ctgaatgaag
    15961 ttgtctccct gactgctgat attcacctca tacccacaag gccacttcac gccatactgt
    16021 tccgtatacc agtgatatgc agaaggaaca ccgttcagaa caccaccgtt aaagccattc
    16081 acttcaacat ccaggcgagt ggacagaacc gtcagcaggt caaacggcat ggttttttcc
    16141 ggcaggacgt tgtcacacag acggttccac cacacagata catcctcgtt actccagatg
    16201 tcgcaccagt cacctctttt tggggtgaaa agagcggtaa tgctttccct ggcatcatcc
    16261 ggtaattctt cccagcgacg ccggccagta ccgctctgca gccagagttc atgcagcatc
    16321 aggcagtttt gctcatccag aagtacaccg tcctgcaggt gtgtcagcca gcgggtgaac
    16381 gcgatattct ccggcgatac aacgccacgc ccggcagccg tcagtccggg gcatggttca
    16441 aaccgcacat cttcagtggt ctgcagaagt ccggcacttc cggccagaaa cagctgaata
    16501 ccttcatttg tggcgcggcg ataaagcggt gtgactgcac cgctggccag tcgtttaatc
    16561 tcagcgatct gtgccggttc accagaaaaa tacatacgat tcgagcacca gtttggcatg
    16621 ggaattctcc tctcacatgc ggcctccatc aggccgtgcc gcacctgtaa ccgggcgacc
    16681 gcatacgaat gcaggtgcgg ccccgaagcc gcagcgcgtg gaggaggaac gacgaaacgt
    16741 gaccgggtga ggaggccggc gacagcgaca cacttgcatc ggatgcagcc cggttaacgt
    16801 gccggcacgg cctgggtaac caggtatttt gtccacataa ccgtgcgcaa aatgttgtgg
    16861 ataagcagga cacagcagca atccacagca ggcatacaac cgcacaccga ggttactccg
    16921 ttctacaggt tacgacgaca tgtcaatact tgcccttgac aggcattgat agaatcgtag
    16981 tctcacgctg atagtctgat cgacaataca agtgggaccg tggtcccaga ccgataatca
    17041 gaccgacaac acgagtggga ccgtggtccc agaccgataa tcagaccgac aacacgagtg
    17101 ggaccgtggt cccagactaa taatcagacc gacgatacga gtgggaccgt ggtcccagtc
    17161 tgattatcag accgacgata caagtggaac agtgggccca gagagaatat tcaggccagt
    17221 tatgctttct ggcctgtaac aaaggacatt aagtaaagac agataaacgt agactaaaac
    17281 gtggtcgcat cagggtgctg gcttttcaag ttccttaaga atggcctcaa ttttctctat
    17341 acactcagtt ggaacacgag acctgtccag gttaagcacc attttatcgc ccttatacaa
    17401 tactgtcgct ccaggagcaa actgatgtcg tgagcttaaa ctagttcttg atgcagatga
    17461 cgttttaagc acagaagtta aaagagtgat aacttcttca gcttcaaata tcaccccagc
    17521 ttttttctgc tcatgaaggt tagatgcctg ctgcttaagt aattcctctt tatctgtaaa
    17581 ggctttttga agtgcatcac ctgaccgggc agatagttca ccggggtgag aaaaaagagc
    17641 aacaactgat ttaggcaatt tggctgtgtt gatacagcgg gtaataatct tacgtgaaat
    17701 attttccgca tcagccagcg cagaaatatt tccagcaaat tcattctgca atcggcttgc
    17761 ataacgctga ccacgttcat aagcacttgt tgggcgataa tcgttaccca atctggataa
    17821 tgcagccatc tgctcatcat ccagctcgcc aaccagaaca cgataatcac tttcggtaag
    17881 tgcagcagct ttacgacggc gactcccatc ggcaatttct atgacaccag atactcttcg
    17941 accgaacgcc ggtgtctgtt gaccagtcag tagaaaagaa gggatgagat catccagtgc
    18001 gtcctcagta agcagctcct ggtcacgttc attacctgac catacccgag aggtcttctc
    18061 aacactatca ccccggagca cttcaagagt aaacttcaca tcccgaccac atacaggcaa
    18121 agtaatggca ttaccgcgag ccattactcc tacgcgcgca attaacgaat ccaccatcgg
    18181 ggcagctggt gtcgataacg aagtatcttc aaccggttga gtattgagcg tatgttttgg
    18241 aataacaggc gcacgcttca ttatctaatc tcccagcgtg gtttaatcag acgatcgaaa
    18301 atttcattgc agacaggttc ccaaatagaa agagcatttc tccaggcacc agttgaagag
    18361 cgttgatcaa tggcctgttc aaaaacagtt ctcatccgga tctgaccttt accaacttca
    18421 tccgtttcac gtacaacatt ttttagaacc atgcttcccc aggcatcccg aatttgctcc
    18481 tccatccacg gggactgaga gccattacta ttgctgtatt tggtaagcaa aatacgtaca
    18541 tcaggctcga accctttaag atcaacgttc ttgagcagat cacgaagcat atcgaaaaac
    18601 tgcagtgcgg aggtgtagtc aaacaactca gcaggagtgg gaacaatcag cacatcagca
    18661 gcacatacga cattaatcgt gccgataccc aggttaggcg cgctgtcaat aactatgaca
    18721 tcatagtcat gagcaacagt ttcaatggcc agtcggagca tcaggtgtgg atcggtgggc
    18781 agtttacctt catcaaattt gcccattaac tcagtttcaa tacggtgcag agccagacag
    18841 gaaggaataa tgtcaagccc cggccagcaa gtgggcttta ttgcataagt gacatcgtcc
    18901 ttttccccaa gatagaaagg caggagagtg tcttctgcat gaatatgaag atctggtacc
    18961 catccgtgat acattgaggc tgttccctgg gggtcgttac cttccacgag caaaacacgt
    19021 agccccttca gagccagatc ctgagcaaga tgaacagaaa ctgaggtttt gtaaacgcca
    19081 cctttatggg cagcaacccc gatcaccggt ggaaatacgt cttcagcacg tcgcaatcgc
    19141 gtaccaaaca catcacgcat atgattaatt tgttcaattg tataaccaac acgttgctca
    19201 acccgtcctc gaatttccat atccgggtgc ggtagtcgcc ctgctttctc ggcatctctg
    19261 atagcctgag aagaaacccc aactaaatcc gctgcttcac ctattctcca gcgccgggtt
    19321 attttcctcg cttccgggct gtcatcatta aactgtgcaa tggcgatagc cttcgtcatt
    19381 tcatgaccag cgtttatgca ctggttaagt gtttccatga gtttcattct gaacatcctt
    19441 taatcattgc tttgcatttt tttattaaat cttgcaattt actgcaaagc aacaacaaaa
    19501 tcgcaaagtc atcaaaaaac cgcaaagttg tttaaaataa gagcaacact acaaaaggag
    19561 ataagaagag cacatacctc agtcacttat tatcactagc gctcgccgca gccgtgtaac
    19621 cgagcatagc gagcgaactg gcgaggaagc aaagaagaac tgttctgtca gatagctctt
    19681 acgctcagcg caagaagaaa tatccaccgt gggaaaaact ccaggtagag gtacacacgc
    19741 ggatagccaa ttcagagtaa taaactgtga taatcaaccc tcatcaatga tgacgaacta
    19801 acccccgata tcaggtcaca tgacgaaggg aaagagaagg aaatcaactg tgacaaactg
    19861 ccctcaaatt tggcttcctt aaaaattaca gttcaaaaag tatgagaaaa tccatgcagg
    19921 ctgaaggaaa cagcaaaact gtgacaaatt accctcagta ggtcagaaca aatgtgacga
    19981 accaccctca aatctgtgac agataaccct cagactatcc tgtcgtcatg gaagtgatat
    20041 cgcggaagga aaatacgata tgagtcgtct ggcggccttt ctttttctca atgtatgaga
    20101 ggcgcattgg agttctgctg ttgatctcat taacacagac ctgcaggaag cggcggcgga
    20161 agtcaggcat acgctggtaa ctttgaggca gctggtaacg ctctatgatc cagtcaattt
    20221 tcagagagac gatgcctgag ccatccggct tacggtactg acacagggat tcgtataaac
    20281 gcatggcata cggattggtg atttcttttg tttcactaag ccgaaactgc gtaaaccggt
    20341 tctgtaaccc gataaagaag ggaatgagat atgggttgat atgtacactg taaagccctc
    20401 tggatggact gtgcgcacgt ttgataaacc agggaaaaga ttcatagcct ttttcatcgc
    20461 cggcatcctc ttcagggcga taaaaaacca cttccttccc cgcaaaactc ttcaatgcct
    20521 gccgtatatc cttactagct tccgcagagg tcaatccgaa tatttcagca tatttaacaa
    20581 catggatctc gcagataccg tcatgttcct gtagggtgcc atcagatttt ctgatctggt
    20641 caacgaacag atacagcata cgtttttgat cccgggagag actatatgcc gcctcagtga
    20701 ggtcgtttga ctggacgatt cgcgggctat ttttacgttt cttgtgattg ataaccgctg
    20761 tttccgccat gacagatcca tgtga
//
LOCUS       DBFIFI010000068        20029 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.068, whole genome shotgun sequence.
ACCESSION   DBFIFI010000068 DBFIFI010000000
VERSION     DBFIFI010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20029
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.068"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(82..828)
                     /gene="cutC"
                     /locus_tag="N7B39_002394"
     CDS             complement(82..828)
                     /gene="cutC"
                     /locus_tag="N7B39_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HGU2422359.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MSRMEKIMAAAGPLAVTFHRAFDMCANPLNTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(842..1408)
                     /gene="yecM"
                     /locus_tag="N7B39_002395"
     CDS             complement(842..1408)
                     /gene="yecM"
                     /locus_tag="N7B39_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGU2422360.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPR
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1624..3357
                     /gene="argS"
                     /locus_tag="N7B39_002396"
     CDS             1624..3357
                     /gene="argS"
                     /locus_tag="N7B39_002396"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HGU2422361.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTHDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEIDEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3399..3443)
                     /locus_tag="N7B39_002397"
     CDS             complement(3399..3443)
                     /locus_tag="N7B39_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2422362.1"
                     /translation="MPIFRIHLDGNKKA"
     gene            3534..4022
                     /gene="yecT"
                     /locus_tag="N7B39_002398"
     CDS             3534..4022
                     /gene="yecT"
                     /locus_tag="N7B39_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HGU2422363.1"
                     /translation="MFKFLVLTLGIISCQVYAEDTLIVNDHDISAIKDCWQKNSGDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSNPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(4142..4534)
                     /gene="flhe"
                     /locus_tag="N7B39_002399"
     CDS             complement(4142..4534)
                     /gene="flhe"
                     /locus_tag="N7B39_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HGU2422364.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELDGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPSLKVQRNEVIVNYR"
     gene            complement(4534..6612)
                     /gene="flhA"
                     /locus_tag="N7B39_002400"
     CDS             complement(4534..6612)
                     /gene="flhA"
                     /locus_tag="N7B39_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HGU2422365.1"
                     /translation="MSNLAAMLRLPTNLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNAVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(6605..7753)
                     /gene="flhB"
                     /locus_tag="N7B39_002401"
     CDS             complement(6605..7753)
                     /gene="flhB"
                     /locus_tag="N7B39_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HGU2422366.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIIKDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPHMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHVEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(7955..8599)
                     /gene="cheZ"
                     /locus_tag="N7B39_002402"
     CDS             complement(7955..8599)
                     /gene="cheZ"
                     /locus_tag="N7B39_002402"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HGU2422367.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRKFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(8610..8999)
                     /gene="cheY"
                     /locus_tag="N7B39_002403"
     CDS             complement(8610..8999)
                     /gene="cheY"
                     /locus_tag="N7B39_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HGU2422368.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(9014..10063)
                     /gene="cheB"
                     /locus_tag="N7B39_002404"
     CDS             complement(9014..10063)
                     /gene="cheB"
                     /locus_tag="N7B39_002404"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HGU2422369.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLNQVS
                     QQMLAKISAGQAIRI"
     gene            complement(10066..10926)
                     /gene="cheR"
                     /locus_tag="N7B39_002405"
     CDS             complement(10066..10926)
                     /gene="cheR"
                     /locus_tag="N7B39_002405"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HGU2422370.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFASLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(10945..12546)
                     /gene="tap"
                     /locus_tag="N7B39_002406"
     CDS             complement(10945..12546)
                     /gene="tap"
                     /locus_tag="N7B39_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HGU2422371.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTTLNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEIIHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(12592..14253)
                     /gene="tar"
                     /locus_tag="N7B39_002407"
     CDS             complement(12592..14253)
                     /gene="tar"
                     /locus_tag="N7B39_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HGU2422372.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVDAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDHVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(14396..14899)
                     /gene="cheW"
                     /locus_tag="N7B39_002408"
     CDS             complement(14396..14899)
                     /gene="cheW"
                     /locus_tag="N7B39_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HGU2422373.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(14920..16884)
                     /gene="cheA"
                     /locus_tag="N7B39_002409"
     CDS             complement(14920..16884)
                     /gene="cheA"
                     /locus_tag="N7B39_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HGU2422374.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKASEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(16889..17815)
                     /gene="motB"
                     /locus_tag="N7B39_002410"
     CDS             complement(16889..17815)
                     /gene="motB"
                     /locus_tag="N7B39_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HGU2422375.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(17812..18699)
                     /gene="motA"
                     /locus_tag="N7B39_002411"
     CDS             complement(17812..18699)
                     /gene="motA"
                     /locus_tag="N7B39_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HGU2422376.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRIIADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(18826..19404)
                     /gene="flhC"
                     /locus_tag="N7B39_002412"
     CDS             complement(18826..19404)
                     /gene="flhC"
                     /locus_tag="N7B39_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HGU2422377.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(19407..19757)
                     /gene="flhD"
                     /locus_tag="N7B39_002413"
     CDS             complement(19407..19757)
                     /gene="flhD"
                     /locus_tag="N7B39_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HGU2422378.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITLLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         4787 a         5522 c         4893 g         4827 t
ORIGIN      
        1 ataaaaaaag gcccctgttg aaattgcagg ggcctggtac gagcaagcat catattgggc
       61 gacatgatgc aacggtaaaa atcatttggc ctgatggcgt tcaatgattc ctttcatttc
      121 agcaaccgcc gccccgtcta cgacataacg cgaatactcg tccgcgtgtg catctgatga
      181 catggacaat ccttgattac gataacgcat cggtgaggct tgccacgctc ccgcggagct
      241 atggacttcc agcactccgg catcgaggaa gtggtgcagg ttttctgcac ggactcctgc
      301 tccggccata atgattggag catcacgatg ggcaataagt tccataattt ttgataaacc
      361 ttgcagtgcg tctgattttt gccctgatgt cagtacccgg gcaatgccca attccgcaag
      421 attattgagt gtatttagag ggttagcgca catatcaaag gcgcgatgaa aagtcactgc
      481 cagcggaccg gcagcagcca ttattttttc cattcgtgac atatcgacat taccgtcaac
      541 atcgagcacg cccgtcacga gtccaggaaa acctaattcg cggaccgtgc gcacatcctc
      601 aagaatggcg gcaaactcac cgtcgctgta gcaaaaatca ccaccgcgtg ggcgaattat
      661 cggatgcaca gggatcgtca cccgctggcg cacggatttc agtacaccca acgacggcgt
      721 taagcccccc tcttttgggg ctgcgcataa ttcaactctg tctgcgccgt tttgctgcgc
      781 cgttagcgcg cattccatgc tgtaacagca aatttccagt aatgccattt ttgctcctta
      841 attacgccga ctgctcgctg gcaacaatct cttcaatgga ccacggatga aatttaatgg
      901 tcgttttgcc atcggtgacc gccagcgtcg gattcggtaa tcgttcatgc tcaccttttg
      961 gcgaactggt tttaactgag atccccggta ggctgagtcc ttcatcagaa agcaatgcca
     1021 gcgcacgggc attcagcgtt tctggatcac ccggcaaaac gatttcaata tgttcccaac
     1081 cttcgtgcgg gtaacgtttt tccctaggcc acggtagctc cacaatagaa aactgccagt
     1141 gtgcaacctg taccggttca tgcaatttaa acagacaaat cggtctgcca ttgatcatat
     1201 tttctgacaa aagctcgcca cactgttcaa acccgcgacg ccagcgctca gcagtggcgt
     1261 tttgatggca acgcaaagaa atgtgatcgg cagtcagcgg agtgatattc aaccccagac
     1321 ggcgggaaag ttcatctaat gcgtgggtaa atcgcggtaa atccgatgca atatcctgca
     1381 gctcgtcgat agattgccag ttcgccataa tcactcttcg tctttcagta aaagcgttaa
     1441 tttaccctgt tgccctgcgc caaccaaccg ctgatttcac gccgcttctg atgcaatagt
     1501 gaaaacggca atacgccacg cgcacgttgc tgacgaaaac agccatttgc agtatactcc
     1561 cgccctaatt tctttaactg gtgcgggcaa tttttgctcg cttcatcaat gtaaggtatt
     1621 ccggtgaata ttcaggctct tctctcagaa aaagtccgtc aggccatgat tgcggcaggc
     1681 gcgcctgcgg attgcgaacc gcaggttcgt cagtcagcaa aagttcagtt cggcgactat
     1741 caggctaacg gcatgatggc agttgctaaa aaactgggta tggcaccgcg acaattagca
     1801 gagcaggtgc tgactcatct ggatcttaac ggtatcgcca gcaaagttga gatcgccggt
     1861 ccaggcttta tcaacatttt ccttgatccg gcattcctgg ctgaacatgt tcagcaggcg
     1921 ctggcgtccg atcgtctcgg tgttgctacg ccagaaaaac agaccattgt ggttgactac
     1981 tctgcgccaa acgtggcgaa agagatgcac gtcggtcacc tgcgctctac cattattggt
     2041 gacgcagcag tgcgtactct ggagttcctc ggtcacaaag tgattcgcgc aaaccacgtc
     2101 ggcgactggg gcactcagtt cggtatgctg attgcatggc tggaaaagca gcagcaggaa
     2161 aacgccggtg aaatggagct ggctgacctt gaaggtttct accgcgatgc gaaaaagcat
     2221 tacgacgaag atgaagagtt tgccgagcgt gcacgtaact acgtggtaaa actgcaaagc
     2281 ggtgacgaat atttccgcga gatgtggcgc aaactggtcg acatcaccat gacgcagaac
     2341 cagatcactt acgatcgtct caacgtgacg ctgacccatg atgacgtgat gggcgaaagc
     2401 ctctacaacc caatgctgcc aggaattgtg gcggatctca aagccaaagg tctggcagta
     2461 gaaagcgaag gggcgaccgt cgtattcctt gatgagttta aaaacaagga aggcgaaccg
     2521 atgggcgtga tcattcagaa gaaagatggc ggctatctct acaccaccac tgatatcgcc
     2581 tgtgcgaaat atcgttatga aacactgcat gccgatcgcg tgctgtatta catcgactcc
     2641 cgtcagcatc aacacctgat gcaggcatgg gctatcgtcc gtaaagcagg ctatgtaccg
     2701 gaatccgtac cgctggaaca ccacatgttc ggcatgatgc tgggtaaaga cggcaaaccg
     2761 ttcaaaaccc gcgcgggtgg tacagtgaaa ctggccgatc tgctggatga agccctggaa
     2821 cgtgcacgcc gtctggtggc agaaaagaac ccggatatgc cagccgacga gctggaaaaa
     2881 ctggctaacg cggttggtat tggtgcggtg aaatatgcgg atctctccaa aaaccgcacc
     2941 acggactaca tcttcgactg ggacaacatg ctggcgtttg agggtaatac cgcgccatac
     3001 atgcagtatg catacacgcg tgtattgtcc gtgttccgta aagcagaaat tgacgaagag
     3061 caactggctg cagctccggt tatcatccgt gaagatcgtg aagcgcaact ggcagctcgc
     3121 ctgctgcagt ttgaagaaac cctcaccgtg gttgcccgtg aaggcacgcc gcatgtaatg
     3181 tgtgcttacc tgtacgatct ggccggtctg ttctctggct tctacgagca ctgcccgatc
     3241 ctcagcgcag aaaacgaaga agtgcgtaac agccgtctga aactggcaca actgacagcg
     3301 aagacgctga agctgggtct ggatacgctg ggtattgaga ctgtagagcg tatgtaatcg
     3361 atttttcgtg agagtgaagc ctgatcaggt ttagccgatc aggctttttt attaccatct
     3421 aaatgtattc tgaaaatagg catgccattg ttttctcgct gttgagtaag aggccagaag
     3481 cgtaatatcc agctccaggg aaacggtaac ggttcaattt aaggaatacc gcagtgttta
     3541 aatttcttgt attaacatta ggcattatct cttgccaggt ttacgcagaa gatacgctta
     3601 tagtaaatga tcatgacatt tcagccatca aagattgttg gcaaaaaaat tcaggtgatg
     3661 atactgacat taacgtgatc aaatcatgtc tgcgacaaga atacaatctc gtcgatgcgc
     3721 aattaaataa agcttatggt gaagcttatc gttatataga acaagtgcca cgcacaggtg
     3781 caaaaaaacc tgatactgaa caacttaact tgcttaaaaa atcacaacga gcctggctgg
     3841 attttaggga caaagaatgt gaattaatcc tttcaaatga ggacgttcag gatttaagta
     3901 acccttattc tgaatcagaa tggctatcat gtatgatcat acagaccaat acgcgaactc
     3961 gccagttgca gctataccgt aactctgaag atttttatcc aagccctttg acaagaggat
     4021 aattcacata tttttggcat gttttgttgc aagctattcc tgataaatga ttgcaacaaa
     4081 acatccagcc tttttcactg agttattaaa catactcgcg agcgcgtaat ttttttgtcc
     4141 ttcagcgata attcacaatc acttcattac gttgtacttt cagcgacgga attaaccgcc
     4201 caccgccagg cacttcccag ataaagcgca acggttctgc tgccgctata ccggaaaagg
     4261 ccacggtggt tccgctctgc ccatctaatt cgacacaacg agattgcgaa cacaagcgaa
     4321 cccgcagtcc tgcaggtgtc gggccgataa gctgataccg ccacgccacc agcgtcatca
     4381 atcctgaagc aggttgtcgc gtagaaagag gtgcagacga catcgactca ccgcgatgat
     4441 ttagcgtaat tccgacacta cttgcctgcc acatcccctc cccggcggct tgcaccagca
     4501 gcggaaacaa taatattgct aataaggttc tcatcattta ccgccaattg tcgccgtcat
     4561 gcggatatgt cggttatcag acagttccag attcgacagc accactaact gcggcaagct
     4621 gcggcgcaga aagcgagaca ataatggtcg cagcgcgtgg ttcaccaata aaactggcgg
     4681 cgcacccaac atctcctgac gggatagcgc ttcctgagtt tgcgccagta aacgatccgc
     4741 cagccctggc tccagtccgc ccccgccctg caacgcctgt agcaacaaac gttccagcgg
     4801 tgtatcgagg ccaataacat ggacttcatc tttgccagga aaccactgct gggtaatcgc
     4861 ccgtcccaac gccacgcgca cgacggcggt taattcatgt ggatcgcttt ggatgggcgc
     4921 atgttccgcc agcgtttcga gaatagtgcg catatcgcga atcggtactt tttcatcgag
     4981 gagattttgc agcactttat gcagtgtggt gagcgtgacg acgccaggga cgagatcttc
     5041 cgtcagcttt ggcatctcct gggcgacgcg atccagcagc tgttgcgcct cctgacgacc
     5101 aaacagctct gcggcatgct ggctaatgag gtggttaaga tgcgttgcta ccaccgtgct
     5161 ggcctcaacc actgtgtacc cctgaatctg cgcctgttct tttagcgcac tttcaatcca
     5221 gatagcattc aggccaaatg ccggatcgac ggtcgcctca ccaggtaacg tcccggcagc
     5281 ggttccaggg ttaatagcca gccagcgccc cggataagca tcaccactgc ctatctccac
     5341 gcctttcatc aaaatgcgat agcgggcagg ttgcagatcc atattgtcgc gaatgtgcac
     5401 cactggcggc agaaatccca tctcctgggc aaatttcttg cggatactgc gtatacggcc
     5461 caacaactca ccatcctgct ggaaatcgac catcgggatc agtcgataac ccacttccat
     5521 tcccagagaa tcttccagtt gtacatcgtt ccacgtcgct tcgacaacgg cattattctc
     5581 tgccattttt accggttttg gttcggcagg cgctttttgt tcgcgtccac ggatccacca
     5641 ggccagcccc agcaatccgg cagtgaacag caaaaatacc aggttcggca ttccaggcac
     5701 caggccgagt aaaccgagca cggcagcgct taacaacata acgcttgggt tactgaaaag
     5761 ctgattcacc atctgctcgc caacatcctg atcggtgctg acacgcgtaa caatgacccc
     5821 cgcggcggta gaaatcacca gagccgggat ttgtgccacc agaccgtcac caatggtcaa
     5881 gagcgtataa ctttccgccg cgtgtcccat gctcatgcca tgttgcagca cgccgaccag
     5941 caacccgccg acaacgttaa tgaccatgat gaggatcccg gcgatggcat cgccgcgaac
     6001 aaacttactt gccccgtcca ttgagccgta aaaatcggct tcctgagtga cttcggagcg
     6061 gcgttttttc gcctcatctt caccaatcaa tccggcgtta aggtcggcgt caatcgccat
     6121 ctgcttaccc ggcataccat cgagaacaaa gcgcgcaccc acttctgcga tacgcccggc
     6181 acctttggta atgaccataa agttgatgat cacgagaatg acaaacacca cgataccgat
     6241 agcgaaattg ccaccaacga ggaagtgacc gaacgcttca accaccttcc ctgccgccgc
     6301 tgcgccggta tgcccttcca ttaaaatgat acgggttgaa gccacgttaa gtgccagacg
     6361 caacagcgtg gtaaacaaca aaatggtcgg aaacgcagca aactcaagcg tgcgctgggt
     6421 aaacatcgcc accagcaaca ccatgatcga caaggcaata ttgaaggtaa acaacaggtc
     6481 gagtatgaat gcgggcagtg gcagcaccat catcgacaag atcaacagga tcaaaatcgg
     6541 tccggcaagg atctgccatt gtgtcgattt caggtttgtg ggcaggcgca gcatcgcggc
     6601 cagattactc atgggtcggt ttctcgttaa taaaatccag ggcttccggc accggaagat
     6661 gagtaggttg tacagggcgc tgtccaccag ccagacgcca gcgtttcagt tgccagaccc
     6721 aggccagcac ttccgccacc gcggcgtaca gttgacccgg gatttgttga ccaatctcca
     6781 catgtcgata cagcgctcgc gccagcggcg gcgcttcaag cgtcgggacg ttattttcag
     6841 cgccaatttc acgaatgcgc agcgcgacca gccctgcacc tttagcgacc actttcggtg
     6901 cgctcatttt gttttcgtca tactgcaacg ctaccgaata gtgggtcggg ttattgacaa
     6961 tgacatccgc tttcggcaca tcggccatca tccgacgccg tgcggcagct cgctgcatct
     7021 gacggatccg ccctttaaca tgggggtcgc cttcgctttg tttgaactca tcacgaatat
     7081 cctgccgcga cattcgcagc tttttcaggt ggctgaagat ttggaaaaag acgtcaaatc
     7141 ccaccattgg aatgacacca agcaccacca gcagtgcgca tagccctacc aaatccatcg
     7201 cattacccat ggcggtaatc ggagactcgg ccattaagcg catcatatgc ggccagtgat
     7261 gccagagaaa aaaccccgtc acgctgccaa ccaggatggt tttcaaaatt gctttaagca
     7321 actccgcgcc agtctgtgcc gagaacatcc gttttatgcc cggtagcggg ttgagtttgg
     7381 aaaacttcgg ctgcaaggat tttccgctaa ataccagccc ccccagcata accggagaaa
     7441 tgagcgccac cagcaccacg ccgctaatca gcggtagcag cgccagcatg gcttctctga
     7501 tcagcagaat aatttgcccg aggatcagat tcgggtcttt gataatactg tgatcaaaat
     7561 gcagcccagc ggagagcatg cccgacaatc gacgggccag cgacacaccg ccaaaccaga
     7621 taacactaac gcccaccagc aaaatcagca gtgaggtcag ttcacgggaa cgcgggattt
     7681 gcccctcttc ccgcgctttt tctagtcggt gaggtgtggg ggcttctgtt ttgtcgtcgc
     7741 tctcgtcaga cacgtcgcca atcctggaaa gagtattaag cgtaaatgat gccagagcgc
     7801 aaagcgttca atggtttgag taaggggcaa aacaggcggg atttagggct tttgctgcca
     7861 cacatcaagc atagtgtgcg tttgtcggat gcggcgtcat cgccttatca gaccgcctga
     7921 tatgacgtgg tcacgccaca tcaggcaata caaatcaaaa tccaagacta tccaacaaat
     7981 cgtccacctg atcctgactg gctaccacac cggctttgct ggtatcgacc tgaggtccat
     8041 taagcaaact ctggttttca cgttttggac gcgactcctg ttccgggatg ttttccaaca
     8101 gcaccatcag caactggcgt tcgatctcct gaatgacatc catcatccgc ttaatgacct
     8161 gcccggtgag atcctgaaaa tcctgcgcca tcatgatttc cagcagttgc gcgttagtaa
     8221 agctggtatg cgcgggtaca tctgccagaa atttccgcgt atctgttacc agttcacggg
     8281 cgtcggcaag gtcaatcgga tcggcaaacc agtcatccca acgttgggtt aacgcttttg
     8341 ctgatttctc catttgatcc tgatgcggtt gtgacgcctc aacactgttc agcgcccgct
     8401 ccgcagcctg ggcggtcatc tgcacaacat agtacaaacg atcgcgcgca tcggggatgg
     8461 cttccgccgc ttcggcaatg gcctgatcca gtcccagttc ccgcaaactg tcgcgcagca
     8521 tacgcgtcag gctgccgatg cgcgcaatga tatcgccagc tgaatgctcg tcagcaggtt
     8581 tgattgatgg ttgcatcata gtcgcatcct cacatgccca gtttctcaaa gattttgttg
     8641 agtttttcct ccagcgtcgc ggcggtaaat ggcttcacca catagccact ggcccccgct
     8701 tgcgccgcag caatgatgtt ctctttcttc gcttctgcag tcaccattaa cactggcaat
     8761 gccgacatcg cgccatccgc acgaatcgtt ttcagcaatt ccaggccatc catgttgggc
     8821 atgttccagt cggagataac aaatccataa ccgcctgcct gcaacttatt gagagcgtcg
     8881 acgccatctt ccgcttcctc aacattattg aatcccagct ctttcagcag gttacgcact
     8941 atgcgtcgca tggtggaaaa gtcatccaca accaaaaatt taagttcttt atccgccatt
     9001 tcacactcct gatttaaata cgtatcgcct gtccggcact aatttttgcc aacatttgct
     9061 ggcttacctg gttaagatcg accacttcgc agacaccacc catattgatg gcctcgcgcg
     9121 gcatgccgaa caccacgcaa cttgcttcgt tttgcgcaag ggtccatgcc ccagcctgac
     9181 gcatcgccaa cattcccgcc gcgccgtcgt tgcccatacc ggtcaggatc accccaaccg
     9241 cattacgccc cgcctgtttg gcgacagaat ggaacaacac atctaccgaa ggccgatgac
     9301 ggttaaccgc cgggccatcg tgaattttga tttggtaatt tgcgccacta cgcgccagct
     9361 ccatatgccg atcgcccggc gcaatatagg catgccccgg caaaacacgt tccccgtctt
     9421 cggcttcttt aaccccgatc tggcaaagct tattaagtct gtcggcaaaa gagcgggtga
     9481 aaccgggcgg catatgctgg gtaattaaca gtgccgggct ggaaagcggc aacggttgca
     9541 gtacgtgacg aattgcctca gttccacccg ttgaagcacc aatcgcaatc agtttttcag
     9601 aactcaacaa cggccctgcc ttcagcgttg tcggtgccga caatggctta tgtgctgcaa
     9661 ggctcgcctt tgctgctgta cgcacctttt cagcaatcat ttcgctgtac gccagcattc
     9721 cttcgcgaat acccagttgc ggtttggtga caaaatctat tgcccccagc tccagcgcgc
     9781 gcagcgtgac ttctgatcct ttgccggtca gggaagaaac catcacaacg ggcattggac
     9841 gcaaacgcat taatttttcg aggaaatcca gtccgtccat ccgcggcatt tcaacatcca
     9901 gcgtcagcac atcgggattg aatttcttaa tcaagtcacg cgcgaccagc ggatcaggcg
     9961 cggtcgccac catttccatg tcgctatggc tgttgatgat ttctgtcatg atctggcgca
    10021 tcagtgccga atcatcgaca gataacaccc tgattttgct catcgttaat ccttacttag
    10081 cgcatacacc gtttgaccac gcagcgtgaa gcggcgctcg aggtggctaa agttttcaga
    10141 gtgacccgca aacagcaatc cgtcgggttt aaggagcgga acaaagcggc gcaaaatctc
    10201 ctgctgggta gtttgatcga agtagatcat gacgttacga cagaagatcg catcaaacgg
    10261 ccccggcacg gtgtactgtt tcgccagtag attcagcgag gcaaaatcaa catagttcgc
    10321 cagctcctga cgcacgcgta ccagcccttc atgcggtccc gtccctcgca tgaaataccg
    10381 ttgcagttgc tgcggcgtca ggtttttcaa ctcttcatgg cgatagatac cgcttctggc
    10441 tttttccagc acttcggtgt cgatatcact ggcaaacact ttccagcgtc cgggcgcggt
    10501 gcccaatgtg tcagccagcg tcatcgcaat gctgtacggc tcttcgccgg tcgaagccgc
    10561 cgcgctccat acgcgatact cgccagaacg gcgacgtgcg tgatccgcga gcagagggaa
    10621 atgatgtgcc tcacggaaaa atgccgtcag attcgtggtc agcgaattga taaacgcctg
    10681 ccactcaccg ctgtgctgat tagattccag caagttcaga taatgaccga aatccgtcag
    10741 tcccagcgaa cgcaaacgac gaaccagtcg gttgtaaacc atgtcgcgtt tatggtcagc
    10801 cagaacgatc ccggctcgtt gatagatcaa ttgacttatc cgccgaaaat gcgcgtcgga
    10861 aagcgccagg cgctcggtca tctgtaacaa taaagacgtt tgcccacagg gcagagatga
    10921 agtcatagcg ccttctcaat cacttcagga taccactggc gcaatttgta actgcgccga
    10981 ctcatgtcgt gctacttcat gttcttcaag ggtaaatacc gccacacgcg acgaaagatg
    11041 gtcggcctga ttcgccagtt gttccgttgc caccgccgct tcttctacca gcgaggcgtt
    11101 ctgctgtgtc acctgatcca tctggctgac agcctgcgca acctgctcaa tcccccgccg
    11161 ttgttcttcc gaggccgaag caatttctcc cataatgtcg ttcacgcggg tcaccgaact
    11221 gacaatatcg gtcatggtcg cggcggcgtt attcaccagt ttcgaaccct gctggacacg
    11281 attgactgac tcttcgatca gccctttgat ctcttttgcc gcctgggcgc ttcggctggc
    11341 aagattgcgc acttctcctg ccactaccgc aaaaccacgc ccctgctctc cggcgcgagc
    11401 cgcttctact gccgcattca gggccagaat attggtctgg aaagcaattc cgtcgataac
    11461 gctgataatg tcgccaattt tttgcgagct ggtggcgatc tcctgcatgg tgtgagtcat
    11521 ggtactgacc tgaacaccgc ccgcctgcgc cgttgtcgcg gcattttttg caagttccga
    11581 cgcctgtcgt gcgttatcgg cgttttgccc taccgtggcg gttaattgct ccatactggc
    11641 ggccgtttgt gccagcgatg ccgcctgctg ttcggtacga cttgagagat cgttattgcc
    11701 tgcgacaatc tccgcgatac caatgtgcat ctcctggctt cccttacgca catcacttac
    11761 cgtcccacgc aaagcctgct gcatggtctt caggctggca aaaatggcgg tgatctcatt
    11821 acgaccatat accgcaatcg gacgcgccag attacccgca gcaatgctgt caaaatggct
    11881 accgataatg gccagtggtt gaacaatcat cttgcgcgtc caccacagcg cactgctgat
    11941 gtagatcgcc gcaacaataa tcatgctgat aaacaccagc gccgaaatct gatagttacg
    12001 ctggctttgc gcactggcgg cttccagcac atggatgatc tccagctgcc aggcctcaaa
    12061 gttaacgtca aacgcattct gtgatccctg caccggcgca gtgaggaaat ccgaaagttg
    12121 attactttca agccaggtcg cctgatgttc gagatcgttg tgccagcggg caaaactttt
    12181 ctccgtctct ttttgcaatg ccctgacgtg ctcgttgccc gcagtcatcg ccataaaact
    12241 tttaaacagc gtggtggatt gcgtcagact ggcacgcgcc gtcgtcatca acgttttaat
    12301 gtcatccgcc ggatagctaa gcgctgtcag agtgcctgct ttgttcagcg tggtactggc
    12361 ctgtaacatt accgcccgag tttgcgctaa tgccgcacgt tgctgattgc tctgctcgac
    12421 ctgattcagt cgttgcaaat cgtcgcgaaa tgcccaaaaa gacatgccgt tactgccaat
    12481 ctgcaagatc ccgcagagaa tcaaaattaa aaacagcgtg gtcgaaattc gaatacgatt
    12541 aaacatcaac gctccccatc aggcggcaat gaccgcgtta gtaaatactc gtcaaaatgt
    12601 ttcccagttt ggatcttgtt cagcaattcg cagtcgtggc tgtgccggtg gttgctcact
    12661 ggcaggacgg gatggtgttt gcggtttatt ggtgagtggg ctggctgcca gacgaaacgc
    12721 ggaaaccgct tgcgttaaac gactagcctg ttcttccagc gcagctgcag cggcagctga
    12781 ttcctgcacc agcgatgcgt tctgttgcgt gacgtgatcc atttccgaaa ccgccaatgc
    12841 gacttgatca atgccacggc tctgttcatc cgatgccgat gcaatctcgc ccataatgtc
    12901 agtcacgcga gtgacagcat cgacgatatt gttcattgtt tccccggcgc tttcgaccag
    12961 caccgaaccg gtatcaacgc gcgagacgga gtcttcaatg agagctttga tctcttttgc
    13021 cgcctgggcg ctacgactgg caagattacg cacttcgccc gccaccacgg caaaaccacg
    13081 gccctgttca cccgctcgcg cggcttcaac agcggcattc agcgcgagga tattagtctg
    13141 gaaggcaata ccgtcgatta cgctgataat gtcggcgatt ttcttcgaac tatcggcgat
    13201 ctcatgcatc gttttcacta cgccatccac cactttgccg ccgtgctggg cggtgtcgga
    13261 ggcactttgc gccagttgcg aggcctggcg ggcgttatcg gcgttttgct tcactgtcgc
    13321 ggtgagctgc tccatgctgg cggcagtttc ttccagcgcg gatgcctgct gttccgtacg
    13381 ggaggaaaga tcggtgttgc ccgccgcaat ttcacgggta ccggcataga tggcatctga
    13441 accttcgcgg acatgagtga cggtgtcagt caaagagcgt tgcatatgtg aaacgctctg
    13501 cgccaggtcg cccatttcac tgcgcccgtc aatggtcagg gtattcgcca ggttaccacc
    13561 ggcgatttcg cgaatgtgag caataatttt tgccagcggt gtaagcaaca tacggcgaat
    13621 gccataccac gccaccagca gaatcaatac caccaccagc gcgataaccg ccagttgcca
    13681 ctgggcaaat cggtaatcat ctgcgttgtc agtgacgata tcgcgataca gtttttcact
    13741 gctgagggcg tactgagcaa acgcttcgcc cattgcattt tgcattccct gggttggctg
    13801 agcgaaataa gctccagtat tgccataatc aagataatca atcagttcag ttaacgctgt
    13861 gtaatagttt ttatattttt catcaatatt acgactggta gcgaccattt caggtaacgg
    13921 tgccatgctt ttgaattttt tataatgagt cgcggcctgg gccaatgttt tcctggcgct
    13981 atcgagcaat tcaactttgg cattactttg ttgattggag gaatccatca tcattcgcac
    14041 cgctgaacga ctcaggttaa tgcgcgtttg cagcattaaa tcccaggttg acgtcagctc
    14101 gccctgctgt tctcgtaatt gattggaaac cacaaagctc ttctggctat ggtgaaggga
    14161 agaaaaaaac agactgccgg aaataagctg taacagtgcg aataccccca gcaccattac
    14221 caacagcgtg actacgcgga tacggttaat cataaggcac cttcctgaaa acaagttgat
    14281 ctcgttatcg gcaaggaagg gggaaacttt attgctgatg ccacccgccg cgaaattgaa
    14341 ataaaacccg atgcgcagat catcgggttc atttcaattg aggaaatcag gagaattacg
    14401 ccacttctga cgccgcgcta tctaacagcg ccatctcttc gctgttcagc agtttttcga
    14461 tgttcaccag aatcaacata cggtcgccca gtgcgcccag tccagtgaga tattctgttg
    14521 aaagcgtcac ggcaaactct ggtgccggac gaatttgctc cgccgtcaat gaaagcacgt
    14581 ctgaaacgcc gtcaaccacg atgccgacca cccgctgtcc gagattcagg acgataacta
    14641 ccgtgttgtc gttatagtcc acatccacct ggctgaactt aattcgtaag tcaacaatcg
    14701 gcacaataac gccgcgcaga ttcgtgacgc ctttgataaa cgctggcgtg ttcgcaatcc
    14761 gtgttacctg atcgtagcca cggatctcct gcactttcag gatatcaata ccgtactctt
    14821 catcaccaag ggtaaatacc agaaattcct ggcctgacgg ctcgctggcc agctttgtta
    14881 cattcgtcat accggtcata ttgttacctt tttactcatt caggcggcgg tgttcgccat
    14941 acgttgttcg cggtttatcg cctgcaaggc ggagacatca acaatcagtg ccacgctgcc
    15001 gtcgccaaga atggtcgcag cagaaatgcc ggggactttg cgatagttac tttcaaggtt
    15061 tttaactaca acctgatgtt gaccaattaa ttgatccacc agcaaggcgt agcggcgacc
    15121 gccactttgt aagatcacca caattccctg ggtagcttcg gttttcgcgc ccgcgacgtt
    15181 gaacactttc cacagttcga cgatgggcag atattcaccc cgcacttcca gcacccgctc
    15241 gccgccggcc agtggatgca gatcggcttc acggggttgc agtgattcca taacagcatt
    15301 cagcggcaga atgaatactt catccgcaac gcgtacggac atgccgtcga ggatggccag
    15361 cgtcagcggc agtaaaatgc ggatcgtagt gccagtaccc tgcttcgact ggatttcaac
    15421 atgaccgccc atctcctgga tattacgttt aacgacgtcc atgccgacgc cgcgcccgga
    15481 gacgtcggtg acctgctctg ccgtggagaa gcctggtgca aatatcagca tcgcgacttc
    15541 gtcgtcgctc atgttttcgc tgacagtcaa accttgcgag gccgcttttg ccagaattcg
    15601 ctcacggttt agccccgccc catcgtcggt cacttcaatg caaatgttgc cgccctgatg
    15661 ttcggcagac agaattaaat ttccgacgct gtttttacct gcggcgagcc gtttttctgg
    15721 cagttcaata ccgtgatcga ggctattgcg taccaggtgg gtcagcgggt cgataatgcg
    15781 ttctatcagg ctcttgtcga gctcggtgga actgcccacc agcgtcagtt ctacctgctt
    15841 gccgagtttt cccgccagat cacgcaccag ccggggatag cgactaaaga catattccat
    15901 cggcatcatg cgaatcgaca tcactgattc ctgcaaatca cgggcgttac gttgtaactg
    15961 ccccatgctg gtaatcaaat caccatgatt aaccgggtcc agttcgctgg aacgctgggc
    16021 aagcatggac tgggtgataa ccagctcgcc gacgaggtta attaattgat caaccttttc
    16081 taccgctaca cggatgctgg tggattcact gctacgcgtc gttttttccc gctccacgcg
    16141 accggttggc gcttgttcgg ctgccagttt aagcactggt ggggtggata tttttggcga
    16201 gacttctact gtttcaaagg taatctgatc ggcttcaatc acaaaacaga gtaccgctgt
    16261 gatgtcatct tcggcgatat cgcccggtaa tattgccgag agagaatccg cccctttcac
    16321 cacgtcagtt aacgttgtca gatgccccag ctcttcttcc agcaggtcga cttcgctggc
    16381 cttcaggcgc gaaaggataa ttcgtcgcgg caactgactg cgactctgct catcttgcgg
    16441 ttcacttttg gcaaccacac ttaatcgggt cactgcggat ggcgtttcgc ctttcgcttc
    16501 taatgccagt tgacgcaagg cctggcagat ataatcgaag ctggcggcat ccggctcttg
    16561 cgactgttta taagcgtcga gctgttcttg catgatgtcc ttcgtttcca aaaacagatt
    16621 gataatgtcg gtgttgagtt gcatctcacc tcgtctggct tcatcgagca ggttttccat
    16681 cagatgcgtg gtttcctgca aaacgctgaa gccaaaagtt cctgcccctc ctttgatcga
    16741 gtgggcagcc cgaaagatgg cattcaattg ttcggcatct ggcgcttccg gctgcaaaac
    16801 cagcagatgc tgctccatat cagccaacag ttcgtccgct tcatcaaaaa atgtctgata
    16861 aaaatcgctt atatccatgc tcacgctgtc acctcggttc ggctgatggc attgtgggaa
    16921 cactgacctg tggtgcaacc tcaggttttt ccagggcgct tactggctca ttctggcttt
    16981 cggcgttttc atgcaaaatg gcctgttcgg cttgtttgtt cagtaccagc aggctgatgc
    17041 gacggttgac ggcatcatca ggtccgcgat cgcttaagcg catcgttgcc gccatgccga
    17101 ccacacgtaa cactttgccg ctatccaacc ctccgaccat cagttcgcgg cgggatgcat
    17161 tggcccgatc ggcagaaagc tcccagttgc tgtaaccttt ctcaccactg gcgtagggga
    17221 aatcatcggt atgacccgaa aggctaatac ggttgggaat accattcaga acgggcgcga
    17281 tggcacgcag aatgtcgcgc atatagggtt cgacgtcggc actgccagtt ctaaacatcg
    17341 ggcgattctg gctatcgatg atctgaatac gtagaccttc ctggaccaga tcgattttga
    17401 gatgtggacg taacgcccgc agtttcggat cggactctat caattggtcg agatcgccac
    17461 gcaatttccg caatcgactt tgctccatgc gttttttcaa ctcttcgatg ttcggctgct
    17521 tattcacttc cccctggctt tgggtgtaat catcgccacc gccgggaatt gggctttcac
    17581 tattagaaat gcgatcgccg cccgtaaccg cagtcgccag tggagtccgg aagtactccg
    17641 caatctgaat caactctttt gggctggaga tggagatcag ccacatcacc agaaaaaagg
    17701 ccatcatcgc ggtcataaag tcggcataag caatcttcca cgatccatgt gctgccccgt
    17761 ggcttttggc tttgcgtcgt ttgacgacaa taatcggatg cgcttgattc ttcatgcttc
    17821 ctcggttgtc gtctgttgtt gcggattttt caccgcacgc acatgctctt ccagttcaat
    17881 aaacgacgga cgttcgctgg aatagagcgt tttgcgacca aactcaacgg cgataggcgg
    17941 tgcgtaaccg ttcagattag aaagcagtgt gactttgacg cactgcatca ttttgctggt
    18001 ttcggcgctt ttctgacgta aaacactcgc taatggggaa ataaatccgt aagccaataa
    18061 aatgccgagg aaagtcccca ccatcgcatg tgcgataagc gcccccagct cggcggcagg
    18121 acgatcggct gaacctaacg cgtgaacgac ccccattaca gccgcgacaa taccaaacgc
    18181 cggaagtgag tccccgacca gcgccagact gttcgccggg acttctgcct cgctttcgtg
    18241 cgtctcaatc tcttcatcca tcagggcttc gatttcgaag gtgttcatgt gaccgctgat
    18301 aatcaggcgc agataatcga cgataaaatc aagcatgacg ctatccgcga tgatgcgtgg
    18361 gtagctggcg aagatctcgc tctcacgggg attttcaata tcacgttcca gcgaaaacat
    18421 ccccatctgc cgcgatttcg ccatcaaccg ataaagcaga gccagcagat ccatatacat
    18481 tgctttggtg tatttggagc gacgaaacag caacggcagc gctttcagcg tgcctttaat
    18541 cgcttttcca ttattgccga caataaacga cccaatccct gcaccggcaa taatcaccag
    18601 ttcagcgggt tgatagagtg ctccaaggct tccaccggtc atcaaataac cgccgaaaac
    18661 tgtaccgaga acaaccaggt aacctaataa gataagcacg acatcatcct tccactgttg
    18721 accatgacag gatgttcagt cgtcaggcgt taacgcgcga ttggggcaaa aaaaagcagc
    18781 ggtacgtcgt taccgctgct ggaatgttgc gccacaccgt atcagttaaa cagcctgtac
    18841 tctctgttca tccagcagtt gtgggataat atcggcagga ttctgggaaa gtttacgtct
    18901 ttttactgcc cgggatggcg gttgacataa gctgcaggca aagctgccaa caggctggtg
    18961 agcgtgggta ataaaattgc cgccgcagca gttgcagctg gaaagttgca gtaatccact
    19021 ttcaacaaac cgcaccaatg tccaggcacg ggttaatgcc agcagcggtc cttcttctgc
    19081 ttgcgggcac tgttcaaggt ataaacggta ggctttgatc accgcatcga cgccattaca
    19141 caaaccggtt ttcagtaaaa actgccatgc attacagaac atcgaagcat gaacgttttg
    19201 ttcccaagtc ataaaccagt cggttgagaa tggcagcatg cctttcggcg gtgggcttcc
    19261 gcgcagttct ttataaagtt ttatcaggcg tccgcgactt aactgtgttt cgctttccag
    19321 catctgcaaa cgagcgccca gggtgatcaa ttccattgcc agctgaatat cccgcgcttc
    19381 ctgaacaatg cttttttcac tcatgatcag gcccttttct tgcgcagcgc ttcttcaggc
    19441 tgattaacat cattcagcaa gcgtgttgag agcatgatgc cggtatgaat ttgctggaga
    19501 tcgtcaacgc gggaatcttg cgtcaacaga gtaatcgtct ggtggctgtc aaaacggaag
    19561 tgacaaacca gctgattggt ttctgccagc ttaaccattt gcggaagagt cagtgccgct
    19621 aatgttgtcg ccatttcttc atttatgccg agacgaaaca tagcggacgc tttgtcctga
    19681 acaatcagac gctgtgcaag tagtaaatat gacaagttga tgtcataaat gtgtttcagc
    19741 aactcggagg tatgcattat tcccacccag aataaccaac tttattttta tgcggtttca
    19801 ccgcaccccg tgatgtcgcc gggaagcccc ggtaaaaaat aattagcatt agaatagttg
    19861 cgataagctg caataagcag agccaccttt ttggtttaat aagtccttac aaatagaagg
    19921 gggtctttac acttatctaa gatttttcct aaatcgacgc aactgtactc gtcactacac
    19981 gcacatacaa cggagggggg ctgcgatttt caataatgtg tgatgcaga
//
LOCUS       DBFIFI010000069        19941 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.069, whole genome shotgun sequence.
ACCESSION   DBFIFI010000069 DBFIFI010000000
VERSION     DBFIFI010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19941
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.069"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            74..592
                     /locus_tag="N7B39_002414"
     CDS             74..592
                     /locus_tag="N7B39_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HGU2422379.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            608..1147
                     /locus_tag="N7B39_002415"
     CDS             608..1147
                     /locus_tag="N7B39_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HGU2422380.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1240..2817)
                     /gene="guaA"
                     /locus_tag="N7B39_002416"
     CDS             complement(1240..2817)
                     /gene="guaA"
                     /locus_tag="N7B39_002416"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HGU2422381.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2886..4352)
                     /gene="guaB"
                     /locus_tag="N7B39_002417"
     CDS             complement(2886..4352)
                     /gene="guaB"
                     /locus_tag="N7B39_002417"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HGU2422382.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4514..5884
                     /gene="xseA"
                     /locus_tag="N7B39_002418"
     CDS             4514..5884
                     /gene="xseA"
                     /locus_tag="N7B39_002418"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HGU2422383.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDLQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQCNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGKVLKKVKQVKAGEMLTTRLEDGWVESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5881..6096)
                     /locus_tag="N7B39_002419"
     CDS             complement(5881..6096)
                     /locus_tag="N7B39_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HGU2422384.1"
                     /translation="MELHCPQCQHVLDQDNGHAYCPSCGKVIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6165..7637)
                     /gene="der"
                     /locus_tag="N7B39_002420"
     CDS             complement(6165..7637)
                     /gene="der"
                     /locus_tag="N7B39_002420"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HGU2422385.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7755..8933)
                     /gene="bamB"
                     /locus_tag="N7B39_002421"
     CDS             complement(7755..8933)
                     /gene="bamB"
                     /locus_tag="N7B39_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HGU2422386.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPAVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8944..9564)
                     /gene="yfgM"
                     /locus_tag="N7B39_002422"
     CDS             complement(8944..9564)
                     /gene="yfgM"
                     /locus_tag="N7B39_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HGU2422387.1"
                     /translation="MEIYENENDQVEAIKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9582..10856)
                     /gene="hisS"
                     /locus_tag="N7B39_002423"
     CDS             complement(9582..10856)
                     /gene="hisS"
                     /locus_tag="N7B39_002423"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HGU2422388.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10967..12085)
                     /gene="ispG"
                     /locus_tag="N7B39_002424"
                     /gene_synonym="gcpE"
     CDS             complement(10967..12085)
                     /gene="ispG"
                     /locus_tag="N7B39_002424"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HGU2422389.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12112..13125)
                     /gene="rodZ"
                     /locus_tag="N7B39_002425"
     CDS             complement(12112..13125)
                     /gene="rodZ"
                     /locus_tag="N7B39_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HGU2422390.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPVVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTAATTPDGAAPLPTDQAGVTTPVADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13410..14564)
                     /locus_tag="N7B39_002426"
     CDS             complement(13410..14564)
                     /locus_tag="N7B39_002426"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HGU2422391.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPAAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14714..15145)
                     /gene="ndk"
                     /locus_tag="N7B39_002427"
     CDS             complement(14714..15145)
                     /gene="ndk"
                     /locus_tag="N7B39_002427"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HGU2422392.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15286..16140)
                     /locus_tag="N7B39_002428"
     CDS             complement(15286..16140)
                     /locus_tag="N7B39_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HGU2422393.1"
                     /translation="MLSHNITIGAECARQRFRFATCHACVEACPAQALTLTEEGIAVD
                     AEQCIDCAVCQFICPQEAIRGVNSPLRYFCNHTLVAPFTTIAPTLEELLLWHKEQGIT
                     AIAIDLSQAKAWERALLLLNECLRQYGEEGWSVVSPHTSEVNVSRRRLFHVQRADVQM
                     TQIQPGLRRLHYLWPQISDARPMMDPQKCQLCGACWRACEQQVFSLNEGHLQINDALC
                     NGCQNCIAVCFHQAMTVELTILPAKIVNLHANRKVCKTCQKSFLTFQQNAQNCLYCQR
                     HRYGMRTP"
     gene            complement(16140..16961)
                     /locus_tag="N7B39_002429"
     CDS             complement(16140..16961)
                     /locus_tag="N7B39_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     family protein"
                     /protein_id="HGU2422394.1"
                     /translation="MHELPLMAFTLLLQASVGMTLWLAFLHRHILITSSARPVMRPPL
                     LLAFLAGAVGLLISTLHLGYPLNAMHALSHFSSSWLSREIIFGALYLALLGLSTLLVM
                     LRKGGWQLLLMLAAVVGIVDVFCMAQVYIHTSIITWQHYNTLIMFLGTVGILGSALVA
                     VFRISGILPQIDALRNGCVLVIALLVLLRLLVQPLWIGDLTANAMQIATLPHAPLAML
                     GQLKPILTLSWGISVIGMMFFAVGGCKKNIPAALFGSVMLVGSEVMLRFVFFSIG"
     gene            complement(16954..17583)
                     /locus_tag="N7B39_002430"
     CDS             complement(16954..17583)
                     /locus_tag="N7B39_002430"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex B
                     subunit"
                     /protein_id="HGU2422395.1"
                     /translation="MSQFKEFASVSDKQLGFFIDSSRCSGCKACQVACKDKNNLEVGR
                     RFRRVYEVNGGGFIATGEGGVSHNVFAYTLSVSCNHCADPICTKNCPTMAMHKRPGDG
                     IVRVNTDKCVGCGYCAWSCPYGAPQMNEQTGQMSKCDFCIDLQAKGEQPICVATCPLG
                     AIKFGPIDELREKYGDVSDVKGLPDSSITQPNLVIKPHQGAEKEASHHA"
     gene            complement(17580..>19941)
                     /locus_tag="N7B39_002431"
     CDS             complement(17580..>19941)
                     /locus_tag="N7B39_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex A
                     subunit"
                     /protein_id="HGU2422396.1"
                     /translation="ERITFPQVSRRSFLQATSALITLPFISSTAKAQSPDASPEVTAP
                     VADKVVPTCSTFDCGGKCDIRAHMRDGVVTQITTLPDNELDPQMPIMRACVRGRGYRK
                     FVYHPDRLKYPMKRVGKRGEGKFERISWDEATTLIADNLKRITQQYGPASRYVHVGTA
                     VSGGTFSGDAMARRLLNLTGGYLEYYHSVSLGNTAAATPYTYGVAASGNSMDTLLDTK
                     LVILWGHNPTETIFGHTNYYFQKMKQNGTRFIVVDPRYSDTVSSLADQWIPLLPTTDN
                     ALMDAMMYVIISENLHDKTFIDTYTLGFDENSMPEGVPANESLVAYLFGAKDGIHKTP
                     EWAEKITHVPAQSIRQLARDYATTKPAALIQGWGPQRHICGERTARGSTLLASITGNV
                     GIKGGWAAGYGGSSNRKFCVGPDMPENPVQAKISIMNWMQAADDASKVTPQDGLKGVD
                     KLDSNIRLLFSLAGNYLANQNPDVHQAAKLLEDESKIEFIVLSDLFMTPSAKYADVLL
                     PETSFMERWNIGETWGTASYLILSEKPIEPDFERRTDYDWLRDVAKKLGVEAEFSQGR
                     DEKQWIEHIWEQTRLAMPDENLPDFATLQKTRRHLFKSAPHIAFEANIRDPQNNPFPT
                     PSGKIEIFSKRLFDMQDPEIPALSHYVPAFEGPEDKLTAKYPLQLITWKGKNRANSTQ
                     YANPWLQEVQTQKLWLNPQDAKQRGISEGDSVKIYNDRGVSIIPVEITPRIIPGVVAM
                     QAGAWWQPDAQGIDRGGCANVLSSTRITALAKGNSHQTMLVEVEKA"
BASE COUNT         4936 a         5409 c         4846 g         4750 t
ORIGIN      
        1 acgcaataca tttactttcc tcttttgatg atcttaaatg tcttattttt cgtaatgtgt
       61 ataacaagga atagtgatga aatttaaaaa atgtcttctg cctgtggcaa tgttagcgtc
      121 attcactctg gcaggatgcc agtcaaatgc tgacgatcat gccgccgatg tttatcaaac
      181 cgatcaactg aataccaaac aagaaactaa aaccgttaat attatttcca ttcttcccgc
      241 aaaagttgcc gtagacaact cccaaaataa acggaacgca caagccttcg gcgcgcttat
      301 tggcgcagtc gctggcggtg ttatcggcca caacgtcggg tctggcagca attccggaac
      361 gacggcaggt gcagttggcg gcggagctgt aggcgcggca gcgggttcta tggtgaatga
      421 taaaacctta gtggaaggtg tttctttaac gtataaggaa ggcaccaaag tgtatacctc
      481 tactcaggtg ggtaaagagt gccagtttac gacaggttta gccgttgtta ttaccacgac
      541 gtataacgaa acgcgtattc agccaaatac caaatgtcct gaaaagagct aataatcagg
      601 aggagtcatg aagaaagttt ttctttgcgc catcttagcc tccttaagct atccggctat
      661 cgcctcatca ttgcaggatc aactctctgc tgtcgcagaa gcggaacagc aaggtaaaaa
      721 tgaagagcaa aggcagcatg acgaatgggt cgcggagcgc aacagggaaa tccagcaaga
      781 gaagcaacgt cgcgcaaatg cccaggctgc cgctaacaaa agagcggcaa cggcagcggc
      841 aaataagaaa gctcgtcagg ataaactgga cgccgaagcc actgcggaca aaaaacgcga
      901 tcaaagttat gaagatgagc tacgcagctt agagattcag aaacaaaaac tggcgctggc
      961 aaaagaagaa gcccgcgtta agcgagaaaa cgaatttatc gatcaggagc tgaagcacaa
     1021 agccgcgcaa actgatgtcg tgcaatctga agctgacgcc aacagaaata tgactgaagg
     1081 cggtcgcgat ctgatgaaaa gcgtgggcaa agcagaagag aataaatcgg acagctggtt
     1141 taattaatcg atgttagtaa cttcaagcct ataattcttg aagataaaaa accctctgta
     1201 gtaacagagg gttttgttca ttcatagtgc agggtcaaat cattcccact caatggtagc
     1261 tggtggcttg ccgctgatgt catacaccac gcgggaaata ccgttcactt cattgataat
     1321 gcggttggaa acgcgaccga ggaaatcgta cggcagatgc gcccagtgtg cggtcataaa
     1381 gtcgatggtt tcgacagcac gcagagagac aacccagtca tacttacgac catcgcccat
     1441 tacgccaacg gaacgtactg gcaggaagac ggtgaacgcc tggctgactt tgtcgtacag
     1501 gtccgcttta cgcagttctt caatgaagat ggcgtcagca cggcgcagca ggtcacagta
     1561 ctctttcttc acttcaccca gaacacgaac gccaaggcct ggtcccggga acgggtgacg
     1621 gtacagcatg tcgtacggca ggcccagctc cagaccaatc ttacgcactt cgtctttgaa
     1681 cagctctttc agcggttcaa ccaggcccat cttcatctct ttcggcaggc cgcccacgtt
     1741 gtggtgagat ttgatgacgt gtgctttacc ggttgcagac gccgcagatt cgataacgtc
     1801 agggtagatg gtgccctgcg ccagccactt cacgtcttcc agtttcagcg cttcttcatc
     1861 gaatacttca acgaaaacgc gaccgatgat tttacgtttt gcttccggat cgttttcgcc
     1921 agccagcgct gacaggaagc gatcttctgc cggtacgtga acaatgttaa gaccaaagtg
     1981 atcgccaaac atatccagaa cctgctctgc ttcgttgaga cgcagcaggc cgttgtcgac
     2041 gaatacgcaa gtcaggtttt taccgatagc gcggtgcagc agcattgcgg ttacggagga
     2101 atccacacca ccagagaggc cgaggatgac tttatcgtcg cctacctgct cgcggatgcg
     2161 agctacagca tcgtcgataa ttttcgctgg cgtccacagg gcttcacact ggcagatatc
     2221 acgcacaaaa cgctccagca tgcgcatacc ctggcgggtg tgagtcactt ccgggtggaa
     2281 ctgtacgcca tagaagcgtt tttcttcgtt agccataatg gcaaacgggc agctttcggt
     2341 gctggctacg gtgacgaagt cggacgggat agcggtaact ttatcgccgt ggctcatcca
     2401 gacatcgagc agcggtttac cgtctgcggt cagcgcatct tcgataccgc gaaccagtgc
     2461 gctgtcgttt acgacttcaa cctgcgcgta gccaaattca cgttcgttag aggcttcaac
     2521 gtgaccgccc aactgcattg ccatggtctg catgccatag caaacgccga ataccggtac
     2581 gcctgcttca aagacatact gcggcgcacg cgggctgttt tcttcagtag tactttccgg
     2641 gccgccggaa agaataatgc cgcttggatt gaagtcacga atttgtgctt ctgtcacatc
     2701 ccatgcccac agttcgcagt aaacacccag ctcacgcacg cggcgcgcaa ccagttgagt
     2761 gtactgagaa ccgaagtcca gaatgaggat gcgatgctta tgaatgtttt ccgtcattga
     2821 cgcttattcc gaggcaagtg aaacagataa tataaatcgc ccgacatgaa gtcgggcgaa
     2881 gagaatcagg agcccagacg gtagttcggg gactctttag taatggtcac gtcgtgaacg
     2941 tggctttcct gaatgcccgc accgctgata cgtacaaact ccgctttagt acgcagttcg
     3001 tcgatagtac cacagccggt caaacccata caggagcgca ggccgcccat ctgctggtga
     3061 atgatctctt tcaggcgacc tttataagct acgcgacctt cgataccttc cggcaccagt
     3121 ttgtcggcag cgttatcgct ctggaaataa cggtcagagg aacctttgga catcgcgccc
     3181 agggaaccca taccacggta agatttgtaa gaacggccct ggtagagttc gatttcaccc
     3241 ggagattctt cagtacccgc cagcatggaa cctaccatca ccgcgcttgc gccagcggcg
     3301 atagctttgg cgatgtcgcc ggagaagcga ataccaccat ctgcgataac cggaataccg
     3361 gtgccttcca gggcttcaac tgcgtcagca acggcagtga tctgcggaac acctacgcca
     3421 gtaacgatac gagtcgtaca gatagaacca gggccgatac cgactttaac cgcgctgcaa
     3481 ccagcttctg ccagagcgcg tgcacctgca gctgttgcca cgttgccgcc gatgatttgc
     3541 agatccggat atttagcacg ggtttcacgg atacgctgca aaacgccttc tgagtgaccg
     3601 tgggaggagt cgatcagcag aacgtcaacg ccagcggcaa ccagcgcgtc aacacgctct
     3661 tcgttacccg cacctgcgcc aactgcagca ccaacacgca gacggccttg ctcgtcttta
     3721 caggcgttcg gtttacgttc cgctttctgg aaatctttca cggtgatcat gccgatcagg
     3781 tggaattcgt catcaaccac cagcgctttt tcaacgcgtt tttcgtgcat ttttgccagc
     3841 accacttcac gggcttcacc ttcacgcacg gtgaccagac gctctttcgg cgtcatgtaa
     3901 acgctaaccg gctggttcag gtcggtaaca aaacgcacgt cacgaccggt gataatcccc
     3961 accagttcgt tttcttcggt aacgaccgga tagcccgcaa aaccgttacg ctcggtcagt
     4021 tctttcactt cgcgcagcgt cgtggttggc agaacagtct gcggatcagt caccacacca
     4081 gattcgtgtt ttttcacacg gcgaacttct tctgcctggc gttcaatgga catgtttttg
     4141 tggataaagc cgataccgcc ttcctgagcc agagcaatag ccaggcgcgc ttccgttacg
     4201 gtatccattg ctgcggaaag catagggata ttcagacgaa tagttttcgt cagctgggtg
     4261 ctgaggtcag cagtattcgg cagaacggta gaatgagcag gaacgaggag aacgtcgtca
     4321 aacgtcagag cttctttagc gatacgtagc atgggcaata tctcgaccag agtggttaat
     4381 aaatattgcc gcggcattat acagagcgta accgattgca tctacccctt tttgcaaaaa
     4441 atgcttgcta tccccgaagg gcgggttact atcgactgaa taacctgctg atttagaatt
     4501 tgatctcgct cacatgttac cttctcaatc ccctgcaatt tttaccgtta gtcgcctgaa
     4561 tcaaacggtt cgtctgctgc ttgagcatga gatgggacag gtttggatca gcggcgaaat
     4621 ctctaatttc acacaaccgg cttccggtca ctggtacttt acactcaaag acgacaccgc
     4681 ccaggtacgc tgcgcgatgt tccgcaacag caaccgccgg gtgaccttcc gcccacagca
     4741 tgggcaacaa gttttagttc gcgccaatat tacgctctac gagccgcgcg gcgactacca
     4801 gataatcgtt gagagtatgc agccggccgg tgaagggctg ctgcaacaga agtacgaaca
     4861 gctcaaagcg aagttgcagg ctgaaggatt gttcgatctg caatacaaaa aaccacttcc
     4921 ctcccctgcg cattgcgttg gtgtgatcac ctcaaaaacc ggtgctgcgc tacatgatat
     4981 tttgcatgtg ttaaaacgtc gcgatccttc tctgccggtg atcatctacc ccaccgccgt
     5041 tcagggcgat gacgcgccgg ggcaaattgt tcgcgccatt gaactggcga atcagtgcaa
     5101 tgagtgcgac gtgttgatcg ttgggcgcgg cggcggttcg ctggaagatt tatggagttt
     5161 taacgacgaa cgcgtagcgc gggcgatttt tgccagccgc attccggtcg tcagcgccgt
     5221 cgggcatgag acggatgtga ccattgccga ttttgttgcc gatctgcgtg caccaacacc
     5281 gtcggctgcc gccgaagtgg tgagccgtaa tcagcaagag ttactgcgcc aggtgcaatc
     5341 gacccgtcaa cggctggaga tggcgatgga ttattatctc gccaaccgca cacgtcgctt
     5401 tacgcaaatt catcaccgat tacagcaaca gcatccgcag ctccggctgg cacgccagca
     5461 aaccatgctt gaacgcctgc aaaaacggat gagctttgcg ctggaaaatc aacttaagcg
     5521 tgccgggcaa cagcagcagc gattaacaca gcggctgaat cagcaaaatc cacagccgaa
     5581 gattcatcgc gcgcaaacgc gcattcagca actggaatat cgtttagcag aaaccctgcg
     5641 cgcacagctt agcgccacgc gtgaacgttt cggtaatgca gtaacgcacc tcgaagccgt
     5701 aagcccactg tcaacgctgg cgcgtggata cagcgttact actgctactg acggcaaggt
     5761 actgaaaaaa gtgaagcagg ttaaagcggg tgaaatgcta accacacgtc tggaagacgg
     5821 ctgggtagaa agtgaagtaa aaaacatcca gccggtaaaa aaatcgcgta aaaaagtgca
     5881 ttaagccagc acgaattcaa cgcgtttttt cgatatcaag ccatgcccgt gctgacagaa
     5941 gtaatctacc gcaccgcagg cttttaacac ctgcaacggt tgatggcagt ccgggcaaag
     6001 agctttcatt tcaataactt tcccgcagga tgggcaatac gcatggccgt tatcctgatc
     6061 aagcacatgc tgacattgtg gacaatgcag ttccatatga attcctccgg tagccacccc
     6121 tctacattca tagagggaat ggcagataaa atacttacgg ataattattt atttttcttg
     6181 atgtgcttca tcagacgctt acgtttacgc atctgggttg gcgtcagggt gttacgcttg
     6241 ttcgcatacg ggttttcccc ttctttgaac tgaatacgaa tcggcgatcc cattacgtcc
     6301 agcgatttgc ggaagtagtt catcaagtaa cgcttgtagg aatcaggcag gtctttcacc
     6361 tgattaccgt gaatcaccac aatcggcgga ttataaccac cggcgtgtgc atatttcagc
     6421 ttcacacgac gaccgcgtac cagcggcggt tggtgatctt caacagccat cgtcatgatg
     6481 cgcgtcagca tagaggtccc cacacgacgg gtggagctgt catacgcttc acgtactgat
     6541 tcaaacaagt taccaacacc actgccgtgc aaggcagaga taaagtgcac acgagcaaaa
     6601 tcgataaagc ccagacggaa gtccagcgtt tctttcacct gctctttcac ttcctgactc
     6661 aggccatccc acttattcac cacaatgaca agtgagcgcc cactattgag aataaagccc
     6721 agcagcgaga gatcctgatc ggaaatacct tcgcgcgcat caatcactaa catcaccacg
     6781 ttggcgtctt caatggcctg caacgttttg attacggaga atttctctac agcatcggtg
     6841 attttgccgc gtttacgtac gccagcggtg tcaatgagca catactcacg tccatcgcgt
     6901 tccattggaa tgtagatact gtcacgcgtc gtaccaggca tgtcataaac aacaacgcgc
     6961 tcttcaccaa gaatacggtt agtgagtgta gacttaccta cgttcggacg acccacaatc
     7021 gccagtttga tcggcagact ttgcgggtcg aagtcgtctt cctcttcttc ttcgccgttc
     7081 tcttccgctt caaattgcgc ccagtattca gcgtcttcgt cgacttcctc ttgcggtgcg
     7141 agatcttcca tccacggcag tagcacatgc tccagcagac ttaatacgcc acgaccgtga
     7201 gacgcggcga tcgggtagat ttcacctaaa ccaagcgagt agaaatcaac cactgcctga
     7261 tcgggatcca gaccgtcagt tttgtttgcc accaggaagg tcggtttttc acgggagcgc
     7321 agatgtttgg caatcgcttc atctgccggc atcaggcccg cgcgcgcatc caccataaac
     7381 agtacgacgt ccgcttcttc aatcgccagc agcgactgtt ccgccatgcg ggtttctaca
     7441 ccgtcttctg tgccatcaat cccgccggta tcaatacaga taaactcacg gccttcaatt
     7501 tccgcacgac cgtacttacg gtcacgagtc agacccggga aatccgcaac cagcgcatct
     7561 cgggtgcgag ttagacggtt aaataacgtg gattttccta cgttagggcg cccgacaagc
     7621 gcgaccacag gtaccatgtt taaagcctca tttttataaa tcatcagaca acgcacgcta
     7681 tattcgcgtc gttgttaaaa acaggaaaac ggcccctgtc caggagccgt tttcaaagtg
     7741 aacgacagag acgattaacg tgtaatagag tacacggttc cgtcttttgc ctggatcagc
     7801 agtttgccgt cagcggcaac cggttcagtc tggaaaccgg aactatcaac tttttgctgg
     7861 gcaacgaaac gaccatcttc gacgttaatc cagtgcaaat aaccttcact gtcaccgacc
     7921 accaggttgc cattatacag caccggagaa gtcagcaggc gatgcagcag atcgctttgt
     7981 gtccacagcg taacgccgcc atcaatggtc aacgccatca cccggtcatt ttgatcgacc
     8041 agatagatgc gattgccgtc gacgatgaaa tcattcaccg aacccagttc gcgtttccac
     8101 ataatctgac cactgcgcag atcaagcgcc gtcaggttac cattataggc cagcgcgaaa
     8161 acaacgccgt taacaacggc gggagtcgtg tcaacatcgc tcagacggtc aatttcggta
     8221 gaaccggtcg cttgggaaat acgctgctgc caaatcatct ggccctgttc catcagcact
     8281 gcgctgacgc gaccattatc gccccccacg acggccgcac caaaagccgt tgccggcgca
     8341 gactcgccac gcaaagagag cgaaggcata tcgaggttaa ctgtccattt gacagcgccg
     8401 tcagcttcgt tcagcgcttg taactgaccg ttactggtgt ggattaacac cagaccgtcg
     8461 ctgaccaccg ggcgcgaaag tgcttcaccc gcgactttag tttgccatgc cacagtacca
     8521 tcgctggtat tcagcgcgta aacctgcgcc ttttcgctgc caatgtagac atgtccacca
     8581 gacacggtca caccgccaga aagtaatgca ggctctttag agaaccagcc atctttctcg
     8641 gccaggctga cagaccagat ttctttgcca tcatccgcat tcagcgcttt tactaaacca
     8701 gcgcggtccg ctgcatagac aacgttgtcc gccagtgccg gatgaagatt ggaatagaag
     8761 ttgccaatgc cgctaccaac ggaagtgctc cacgccgtgg tcggcgtaaa ctggttttca
     8821 acggttggca atggggacat ctttaccaca tcttcttcgc tgttaaacag cgaacagccg
     8881 cttaaaaggg taacggaaag cagtcctggc agcagtaatt tacgcaattg catcgggtcc
     8941 ctctcagatg gacaaattat taattttcat ctgcatcatt tcgctcagtg ccggagtaac
     9001 atcgcttttc acgcctgctt cccatgcact acgcgcacct tgcttatcac ctttgctcag
     9061 caatgcttca ccacgcaggt cggcaacaat ggcagcccac ccttcacctt tgatggtatc
     9121 aagggttttc agcgcggcat cagcctgctt gagctgtacc tgaacgcgag caagacgcag
     9181 atttatcacg gctttgagat tttcatcgct cgtgtctgcc agcccctgtt gtaactgggc
     9241 ggcagctttc tctagttcat ttttgtcaac aaattgctgc gcaagttcca aagaagccag
     9301 cgcaccataa gtatttttat tttcagcagc aaatttttcc gccgccggga tgctatccgg
     9361 tttgccttcg ctcactgcgg taaccgcatt ttgataggca agagaagcgg agcgtgcaga
     9421 atcaacctga tggctgttcc agtagcgcca gccaatcagt gcgccaacgc ccaaaatcac
     9481 cccaacagcc agtgctttgc cattttcagc aaaaaagcgt ttaatcgctt ctacctggtc
     9541 gttttcgttc tcgtaaattt ccacgctgtc cttctccttc cttaacccag taacgtgcgc
     9601 aaatgcgcgg ctacgctatc ctgcgcaact gccgtttgct caccagagcg caaatccttc
     9661 actactgctg tgccgttagc cacttcagac tcacccagca ccacagcaac gcgggcaccc
     9721 catttatcag cacgggcaaa ctgtttctta aagttgccgc cgccgtggtt ggtcatcaat
     9781 ttcacgcccg gtaattcatc acgcagacgc tcagctaatg ccatagccgc agattgtgta
     9841 tcagcacctg aagccaccag gtatatatcg acaacaggat cggctttaaa ttccggatta
     9901 acggcctgta ctaacaatac aagacgttcg aggcccatcg caaaaccgac agccggtgtt
     9961 gcacggccgc ccagttgttc cacaagaccg tcataacgac cgcctgcaca cacggtgccc
    10021 tgggagccga gactgttagt cacccactcg aaaacggtac ggttgtagta atccagacca
    10081 cgcaccagac gctggtttac ggtgtaagcg atccccgcgc tctcaagcag tttgcacaga
    10141 ccggcaaaat gctcacgaga ttcctcgtcc agataatcac ctaatgccgg agcgtcgttg
    10201 agaagcgcct gcacttccgg attttttgaa tccagcacgc gcagcgggtt agtgtacatg
    10261 cggcgtttgc agtcttcgtc cagcttttct ttatgctgct caaggaatgc caccagcgca
    10321 tcgcggtaat tggcgcgtgc ttccagcgaa ccgatagaat tcagctcaag agttacgtgt
    10381 tcggaaatac ccagcgcgcg ccaccagcgg gcggtgagca taatcagttc agcgtcgata
    10441 tccggacctt gcagaccgaa aacttcgcag cccaactgat ggaactgacg ataacgccct
    10501 ttctgcggac gctcgtgacg gaacatcggc ccgatatacc acagacgctg ttcctgattg
    10561 tacagaagac catgctcgat gccggcgcgt acacagcccg ccgtcccttc agggcgcaga
    10621 gtcaggctgt cgccattgcg atcctcaaag gtgtacatct ctttttcaac cacgtcggtg
    10681 acttcaccaa tcgcacgttt gaatagcggg gtctgctcta caatcggcaa gcggatttca
    10741 ctgtaaccgt agctgccgag cacgtttttc agtgtgcctt caatgcgctg ccagatggcc
    10801 gtttcgccag gcaggtaatc gttcatgccg cgaatggctt gaatgttttt tgccacgttt
    10861 attctctttc tgaatataaa aatgaaccct caacgcttcc ctcaatgttt cgggagccat
    10921 gcgggttcaa tcatacacgg gaagcgaggc gcttcccatc acgttattat ttttcaacct
    10981 gctgaacgtc aattcgacgc gcttcgtcca gctgactggc tttcgcacga atgcgtgcct
    11041 ccagctggtc gatcatatcg ttgttgtcca gacggtcttt gcgcacgcca tcttcataga
    11101 gaccgctttt cttgttgccg ccggtgacgc cgagtgtaga aaccagcgcc tcacccgggc
    11161 cattcaccac gcagccgata atcgaaacgt ccatcggagt gatgatatct tccaggcgtt
    11221 gctccagcgc gttaactgta ccgataacat caaattcctg acgcgaacag gtcggacagg
    11281 cgatgaagtt gatacctcgc gaacggatac gaagcgattt caaaatatcg aaaccgactt
    11341 tgatctcttc gaccggatcg gccgccagtg atacgcgcag cgtgtcgccg atgccttcag
    11401 acagcagcag acctaaacca atggcggatt ttactgcccc gctgcgcgcg ccacccgctt
    11461 cggtgatccc cagatgcagc ggctgatcga tttgttttgc cagcaaacga taagactcaa
    11521 cagcgaggaa gacgtcagac gctttcacgc tgactttgaa ctgatcgaag ttcaggcgat
    11581 cgagatgatc aacatggcgc atggcggatt ccagcaacgc ctgcggcgtc ggttcgccat
    11641 acttttcttg cagatctttt tccagcgatc cggcgttaac gccaatacgg atcggaatgt
    11701 ttttatcgcg cgcacagtca accaccatgc gaatacgctc ttcattaccg atattgccag
    11761 ggttaatacg cagacaatcg acgccgtatt ccgctacttt cagcgcaatg cgatagtcga
    11821 agtggatgtc agccaccagc ggcacgttaa cctgctgttt gatgagtttg aacgcttctg
    11881 ccgcgtccat cgtcggtacg gagacacgga cgatatcagc gccaacgcgt tccagcgcct
    11941 tgatttgatt gaccgttgct tcgacatctg tcgtacgcgt attggtcatg gactgtacgg
    12001 cgatgggagc accatcgcca atcggcacat tcccaacgta aatacgtgtt gattttctac
    12061 gttgaattgg agcctggtta tgcatgaaaa atctcccgcg ttacccgtct gttactgcgc
    12121 cggtgattgt tcggcattga gggtcagacg cgcaacctgg ttagttctga taaaacgact
    12181 cagatcgaca ggtttccctt gatactggat ctgtactgcg gctggcgcac caattttcag
    12241 tttgtacggt gcctggccgg ttaagttcaa attaccgtct ttacgctgca taccgctaaa
    12301 caatttttta ccggtagcat cagtgacctc cagccagcaa tcggcagtaa agttcatcac
    12361 cagcgcattc ggatcagcca ccggcgtggt cacgccagcc tgatcggttg gcaacggcgc
    12421 agcaccatct ggcgttgttg ctgccgttgg tgccggggtc gcggcggtat caacatttgc
    12481 ctgcgaaggc gaaacaaccg cattctgttg cggatcaaca gctggtgctg gcgcagttac
    12541 gacaggtgtt tgcgtgttgg ttgcggtagt atccacagac gctggcggcg tggacgttgt
    12601 agccgggtct gtagttgtcg acgtatttaa cggaacactc tgcccctgct cgctattact
    12661 gctcagttcc gccgaagatt gatcggccat agtggtgatc tcttcctgct gagctttgtg
    12721 gtcttgccac caccaggcac cgctcaggcc gataaccaca aacaacacca gccaggtgaa
    12781 ggtcatcagc cagccgtcgc gttttttgcg gcgtttaccg agggaaaaac tctgcatcgg
    12841 cgcaactttt gcagcccgta gtggagcctg cttttccagc cctggcagca gttcttcttc
    12901 tggaatatgt accagacgcg cataagagcg gatatatccg cgcaggaatg ttgaagcaag
    12961 atcggcgggt gccttatctt cttcaatgtc gcgtaccgtg gaaaccttca ggcaaagtcg
    13021 ctcggcaacg gcctgctgac taagtcctag ttgttcgcga gcattacgca ggcgagcgcc
    13081 ggtagtaagt gcttcatttt ggtcgtgcgt ggcttcagta ttcattcgct gcaggtacgt
    13141 ttaaatgaga attgagatgc cggtgaacca tcatgctcac ccacaccgcg aaacatccgt
    13201 taagttaacc gttatcatac agtataagac cgtctgtgtg ctcgtgacaa agcccacatt
    13261 cactaaggct aacttactgt tgcatcgtta catactgcct taaagtcagc aaaaacgcac
    13321 cgttaatatt gaccagacaa ttgcaactta atacttcatt cataaaacat tacgccacgg
    13381 tacataaagt aaccgtggcg taatggctat cagaccgctt taatgtcgat ggcttcaccc
    13441 tgcatccgtt tacgcagggt acgtttcgta cggtcgataa catcgcccgc caactgacca
    13501 caggcagcat cgatatcatc accacgcgtt ttacgcacaa tagtggtgaa accgtagctc
    13561 atcagcactt ttgagaaacg gtcgatacgg ctgttcgagc tgcgtccata cggcgcagcc
    13621 gggaacgggt tccacgggat caggttgatc ttacacggcg tatctttcag cagttccgcc
    13681 agttggtgcg cgtgttcagt gccgtcgtta acgtggtcaa gcatcacgta ttcaatagtg
    13741 actcggccct gattggcgtt ggatttctcc agataacggc gcaccgcagc aaggaacgtt
    13801 tcgatattgt actttttgtt gatcggcaca atttcgtcac gaatttcgtc gttcggcgcg
    13861 tgcagggaaa ttgccagcgc aacatcgatc atatcgccca gtttatccag cgctggaact
    13921 acaccggaag tggaaagcgt cacgcgacgt ttagacagac caaaaccgaa atcatcaagc
    13981 atgatttcca tcgccggaac gacgttgttc aggttgagca gcggctcgcc catgcccatc
    14041 atcactacgt tagtgattgg gcgctgaccg gtgacttttg ctgcgccgac gattttcgcc
    14101 gcacgccaca cctggccaat aatttccgat acccgcaggt tgcggttaaa gccctgctgg
    14161 gcggtggaac agaatttaca ctccagcgca caccccacct gcgaagagac gcagagcgta
    14221 gcgcggtcgt cttccgggat gtacaccgtt tcgacacgct ggtcacccac ggcaatcgcc
    14281 catttaatgg tgccgtcaga tgaacgctgt tcttcaacca cttccggtgc gcggatctcc
    14341 gccacctctt tcagtttgcc gcgcaacact ttgttgatgt cggtcatctc atcaaaattg
    14401 tcgcagcaat agtgatacat ccacttcatc acctgatcgg cgcggaaggg tttttcacct
    14461 aaatctttaa aaaactcccg catctgctga cgattgagat ccagcaggtt gatttttcca
    14521 tctttggtgg tgacgttttc aggtgtgact aattgttcag acatatgcta ttccggcctc
    14581 gttattacac gttatggccc ctggagggtt gaaaaaagaa acgccccggt gagcggctgc
    14641 tcgtccgggg gcgctgcatt gtacaaattc tggcgcacgg atgccacgtt tgcacgcggc
    14701 atttacgaaa ttattaacgg gtgcgcgggc acacttcgcc ttcgccgaag aaataagcga
    14761 tttcgcgagc ggcagattcg acggaatcag aaccgtgggt accgttttcg gtcaggctgt
    14821 cagcgtaatc agcgcgcaga gtaccggcca gtgcgtttgc cggattggtc gcgcccagca
    14881 gatcgcggtg acgctgaacg gcgttttcac cttccagcac ggaaaccacg atcgggccag
    14941 aggtcatgaa ttcaaccaga ccatcaaaga acggttttcc atcgtgttca gcataaaagc
    15001 cacgtgcctg ttcaacggtc aggtgcagca ttttggtgcc gacaattttg aaccctgcag
    15061 cttcaaagcg cgcaaagata ttaccaatga cgttttttgc taccgcgttc ggtttgataa
    15121 tggaaaaagt acgttcaata gccattttta cctctgtaaa ttgttctgtt gttgtctgta
    15181 ccagcgtacg agatggcgcg gattataatg agcaacagag ccgttgacta ttgatgaagg
    15241 taacattttt ttaaaataaa atgagtttta gcaacaactc gacttttatg gtgttctcat
    15301 cccataacga tgacgttggc aataaagaca attttgcgca ttttgctgaa aagtaagaaa
    15361 ggatttttga caggttttac agactttgcg attagcatga agattcacaa tttttgccgg
    15421 tagaatcgtc aattcaacgg tcattgcctg atgaaaacaa acagcaatac aattttggca
    15481 tccgttacac aaggcatcat ttatttgtaa atggccttcg ttaagcgaaa agacctgttg
    15541 ctcgcaggcc cgccagcatg caccgcaaag ctgacacttt tgaggatcca tcatcgggcg
    15601 agcatcacta atctggggcc ataaataatg taaccgacgt aatcctggtt gtatttgtgt
    15661 catctgaaca tccgcacgct gcacatggaa caaacgacgc cgggaaacat tcacctcact
    15721 ggtatgtgga gataccacag accagccttc ctcgccatat tgccgtaagc attcgtttaa
    15781 cagcaataat gcgcgctccc atgccttcgc ctgactcaga tcaatcgcga tcgctgtgat
    15841 cccctgctct ttatgccata gcaaaagttc ttccagcgtc ggcgcaatag tggtgaaagg
    15901 tgcgaccagg gtgtgattgc aaaaataacg taaaggggaa ttaacgccac gaatagcctc
    15961 ttgcggacaa ataaactggc aaaccgcaca atcaatacat tgttccgcgt cgaccgctat
    16021 gccctcttct gtcagcgtta gcgcctgtgc cgggcaagcc tcgacacagg catgacatgt
    16081 tgcaaaacga aagcgttggc gcgcgcattc cgcgccaatc gttatgttat gacttaacat
    16141 tagccgatac taaaaaagac aaaacgcagc ataacttcac tccccactag cattacgcta
    16201 ccgaataacg ccgccgggat attcttttta caaccaccga cagcaaagaa catcatccca
    16261 atgacggaaa ttccccagct tagtgtcagt atgggtttta actgtcccag catcgctaat
    16321 ggcgcatgtg gcaatgtggc tatctgcatc gcatttgccg ttaaatcccc tatccacaac
    16381 ggctgaacca acaaacgcag cagaaccagg agagcgatca ccaacacgca tccattacgc
    16441 agggcatcaa tctgcggcaa aattccgcta atacgaaaca ctgctaccag cgctgaaccg
    16501 agaattccca ctgtacctaa gaacataatc agcgtgttgt aatgctgcca ggtgataatc
    16561 gacgtatgga tgtaaacttg cgccatgcag aaaacatcaa caatcccgac cactgccgct
    16621 agcataagca ggagttgcca gccgcctttg cgtaacatga ccagtaatgt ggataaaccc
    16681 aatagcgcca gatacagcgc accaaagata atttcccgac tcagccatga actggaaaag
    16741 tggctcagcg catgcatcgc attaagtgga taacccaggt gtaaggtaga aatcagtaac
    16801 cctacggcac ctgccaaaaa agccaacagt aatggcggcc gcataaccgg gcgggcgctg
    16861 gaggtgatta aaatatgacg atgaagaaac gccagccaca acgtcatacc gaccgatgcc
    16921 tgtaacaaca gcgtaaaagc cattaacggt aattcatgca tgatgtgacg cctccttttc
    16981 cgcgccctga tgcggtttaa tcaccagatt tggctgcgta atcgacgaat caggtaaacc
    17041 tttcacatca ctgacatcgc catacttttc acgcaactca tcaatagggc cgaacttaat
    17101 cgcccccaac gggcacgttg ccacgcaaat gggttgttct cctttagctt gcagatcaat
    17161 acagaaatcg catttcgaca tctgcccggt ctgctcattc atttgtggtg cgccgtaagg
    17221 acaggaccag gcgcagtaac cgcaaccgac gcacttatca gtattcacgc gcacaatacc
    17281 gtctccggga cgcttatgca tcgccatcgt cgggcaattt ttggtacaga tcggatccgc
    17341 gcaatggtta caggaaacag aaagggtata agcaaagaca ttatgactca caccgccctc
    17401 tccagtggcg atgaagccac ctccgttcac ttcatacacg cggcgaaaac gtcgcccaac
    17461 ctcaaggttg tttttgtctt tacaggccac ctgacacgct ttacagcccg aacaacgcga
    17521 agagtcaata aagaacccta attgtttgtc gctgacagat gcaaattctt taaattggct
    17581 catgctttct caacctccac cagcattgtt tgatgcgaat tccctttcgc cagcgccgta
    17641 atgcgggtcg aactcagtac attggcacag cctccgcgat cgataccttg tgcatccggt
    17701 tgccaccatg cccccgcctg catagcaact acgccaggga tgatacgggg ggttatttcc
    17761 accgggataa tgctcacacc acgatcgtta taaattttta cgctatctcc ttcgctaatc
    17821 ccgcgctgtt ttgcatcttg cggattcagc cagagttttt gcgtctgaac ttcttgcagc
    17881 cacgggttag catactgggt ggaattagca cggtttttcc ctttccaggt gatgagctgc
    17941 aacgggtatt tcgccgtcag tttatcttct ggcccttcaa aggcagggac atagtgcgaa
    18001 agcgcgggaa tttctggatc ttgcatatca aaaagacgct tagagaagat ctcaattttg
    18061 ccggaaggcg tcgggaaagg attattctgc ggatcgcgaa tatttgcttc gaaagcgatg
    18121 tgcggcgcgc ttttgaaaag atgtcgacgt gttttttgca gggttgcgaa gtccggcaaa
    18181 ttttcatccg gcatcgccag acgagtttgc tcccagatat gctcgatcca ttgcttctca
    18241 tcacgtcctt gactgaactc ggcttcgaca cccagttttt tcgccacgtc acgcaaccag
    18301 tcataatcgg tgcgacgttc aaaatccggc tcaatcggtt tttctgacag aataagatag
    18361 ctcgcagttc cccatgtttc gccgatattc cagcgttcca taaagctggt ttccggcagc
    18421 aacacatcgg catatttcgc gctcggagtc atgaacagat cgctgagtac gatgaattct
    18481 attttagatt cgtcttcaag gagcttcgcg gcctggtgga cgtccgggtt ctggttcgcc
    18541 agataattac ctgccagtga gaacagcagg cggatattac tgtcgagttt atcgacacct
    18601 ttaagcccat cttgcggcgt aactttagaa gcatcatccg ccgcctgcat ccagttcata
    18661 atcgagattt tcgcttgtac cggattttct ggcatatctg gtccaacgca gaatttacga
    18721 ttagaactac cgccgtaacc tgccgcccag ccacctttga tgccgacgtt accggtaatc
    18781 gaggccagta aagttgaacc tcgggcggtg cgttcgccgc aaatatgtcg ctgtggcccc
    18841 catccctgaa tcaacgctgc gggttttgta gtggcgtaat cgcgtgccag ttgacgaatg
    18901 gattgtgctg gaacgtgggt aattttttct gcccattccg gcgttttgtg tatcccatct
    18961 tttgcgccaa aaaggtaggc aaccaacgat tcgttggctg gtacgccttc cggcatggag
    19021 ttttcatcaa aaccgagagt atatgtgtcg ataaatgtct tatcgtggag attctcgctg
    19081 ataatgacgt acatcatcgc gtccatcaac gcattgtccg tggtcggcaa cagcgggatc
    19141 cactgatcgg caagtgaaga tacggtgtcg gaatagcgcg gatccaccac gataaaacgt
    19201 gtaccgtttt gcttcatttt ttgaaaatag taattggtgt gaccgaatat ggtttccgtt
    19261 gggttatgcc cccagagaat caccagtttg gtatccagaa gagtatccat cgagttaccg
    19321 ctcgcggcca cgccatacgt gtaaggcgtg gccgccgcag tattacccag actaactgag
    19381 tgataatatt caagataacc accggttaaa tttaacagcc gacgcgccat cgcatcgcca
    19441 gaaaatgttc caccagaaac agccgtcccg acatggacat aacgtgaagc tgggccatat
    19501 tgttgcgtga tccttttcag gttatcggca attaaggtcg tcgcctcatc ccatgagata
    19561 cgctcaaatt taccctcgcc tcgtttacct acccgcttca tcggatattt caaacggtct
    19621 ggatgataaa caaatttacg atagccgcga ccacgaacac aggcgcgcat aatcggcatt
    19681 tgtggatcca attcgttatc gggaagcgtg gtaatttgcg tcaccacgcc gtcgcgcata
    19741 tgcgcccgaa tatcgcactt accaccgcag tcaaatgtgc tacaggtggg cacgacttta
    19801 tcagctactg gagctgtaac ttctggcgac gcatctgggg attgcgcctt tgcggtagaa
    19861 gaaataaaag gcaaagtgat aagcgcggaa gtcgcttgta aaaaactacg tcgtgatact
    19921 tgcggaaatg taatgcgttc c
//
LOCUS       DBFIFI010000070        19801 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.070, whole genome shotgun sequence.
ACCESSION   DBFIFI010000070 DBFIFI010000000
VERSION     DBFIFI010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19801
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.070"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            183..446
                     /gene="ykgM"
                     /locus_tag="N7B39_002432"
     CDS             183..446
                     /gene="ykgM"
                     /locus_tag="N7B39_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HGU2422397.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
     gene            446..586
                     /gene="ykgO"
                     /locus_tag="N7B39_002433"
     CDS             446..586
                     /gene="ykgO"
                     /locus_tag="N7B39_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HGU2422398.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(656..847)
                     /locus_tag="N7B39_002434"
     CDS             complement(656..847)
                     /locus_tag="N7B39_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2422399.1"
                     /translation="MQAASQREETEWRVQSKRGLMPAYRGEAGQQVNINIMEYSERNV
                     RQLTLNEQEDTSPGKLMLV"
     gene            1672..2214
                     /gene="ecpR"
                     /locus_tag="N7B39_002435"
                     /gene_synonym="matA"
     CDS             1672..2214
                     /gene="ecpR"
                     /locus_tag="N7B39_002435"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HGU2422400.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLTEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMLPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKLYKLVQ"
     gene            2289..2876
                     /gene="ecpA"
                     /locus_tag="N7B39_002436"
                     /gene_synonym="matB"
     CDS             2289..2876
                     /gene="ecpA"
                     /locus_tag="N7B39_002436"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HGU2422401.1"
                     /translation="MKKKVLAIALVTVFTGMGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            2934..3602
                     /gene="ecpB"
                     /locus_tag="N7B39_002437"
                     /gene_synonym="yagY"
     CDS             2934..3602
                     /gene="ecpB"
                     /locus_tag="N7B39_002437"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HGU2422402.1"
                     /translation="MKKHLLLLALLLSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVISMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGTLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            3628..6153
                     /gene="ecpC"
                     /locus_tag="N7B39_002438"
                     /gene_synonym="yagX"
     CDS             3628..6153
                     /gene="ecpC"
                     /locus_tag="N7B39_002438"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HGU2422403.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMALANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNFGIYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGTLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRTQRVNKLFSRGRGVGAPLAWQVWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTLSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSSIGSI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQNVYSGTFGSLGLRAGIQRYNNGDSNANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYVNTNLTANGSVGWQGKNIAASGRTDGNAGVIFNTGLEDDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            6143..7786
                     /gene="ecpD"
                     /locus_tag="N7B39_002439"
     CDS             6143..7786
                     /gene="ecpD"
                     /locus_tag="N7B39_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HGU2422404.1"
                     /translation="MRVNLLIAMIIFALIWPVTALRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYNGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDTSGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSRSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNQGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            7755..8465
                     /gene="ecpE"
                     /locus_tag="N7B39_002440"
     CDS             7755..8465
                     /gene="ecpE"
                     /locus_tag="N7B39_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HGU2422405.1"
                     /translation="MFRRRGVTLTKALLTAVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPQDNRERYYRVSFREVPTRNLTKRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDQVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPEDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            8778..9107
                     /gene="ykgJ"
                     /locus_tag="N7B39_002441"
     CDS             8778..9107
                     /gene="ykgJ"
                     /locus_tag="N7B39_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HGU2422406.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGVVNEACNRARA
                     KYGLTPL"
     gene            complement(9355..9969)
                     /gene="yagU"
                     /locus_tag="N7B39_002442"
     CDS             complement(9355..9969)
                     /gene="yagU"
                     /locus_tag="N7B39_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HGU2422407.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNAAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
     gene            10387..11076
                     /gene="paoA"
                     /locus_tag="N7B39_002443"
     CDS             10387..11076
                     /gene="paoA"
                     /locus_tag="N7B39_002443"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047770 - carboxylate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase iron-sulfur subunit PaoA"
                     /protein_id="HGU2422408.1"
                     /translation="MSNQGEYPEDNRVGKHEPHDLSLTRRDLIKVSAATAAAAVVYPH
                     STLAASVPAATPAPEIMPLTLKVNGKTEQLEVDTRTTLLDALRENLHLIGTKKGCDHG
                     QCGACTVLVNGRRLNACLTLAVMHQGAEITTIEGLGSPDNLHPMQAAFIKHDGFQCGY
                     CTSGQICSSVAVLKEIQDGIPSHVTVDLVSPPERTADEIRERMSGNICRCGAYANILA
                     AIEDAAGEIKS"
     gene            11073..12029
                     /gene="paoB"
                     /locus_tag="N7B39_002444"
     CDS             11073..12029
                     /gene="paoB"
                     /locus_tag="N7B39_002444"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase FAD-binding subunit
                     PaoB"
                     /protein_id="HGU2422409.1"
                     /translation="MKAFTYERVNTPAEAALSAQRVPGAKFIAGGTNLLDLMKLEIET
                     PTHLIDVNGLGLDKIEVTDAGGLRIGALVRNTDLVAHERVRRDYAVLSRALLAGASGQ
                     LRNQATTAGNLLQRTRCPYFYDTNQPCNKRLPGSGCAALEGFSRQHAVVGVSEACIAT
                     HPSDMAVAMRLLDAVVETITPEGKTRSITLADFYHPPGKTPHIETALLPGELIVAVTL
                     PPPLGGKHIYRKVRDRASYAFALVSVAAIIQPDGSGRVALGGVAHKPWRIEAADAQLS
                     QGAQAVYDALFASAHPTAENTFKLLLAKRTLASVLAEARAQA"
     gene            12026..14224
                     /gene="paoC"
                     /locus_tag="N7B39_002445"
     CDS             12026..14224
                     /gene="paoC"
                     /locus_tag="N7B39_002445"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HGU2422410.1"
                     /translation="MKFDKPAGENPIDQLKVVGRPHDRIDGPLKTTGTARYAYEWHEE
                     SPNAAYGYIVGSAIAKGRLTALDTDAAQKAPGVLPVITASNAGALSKGDKNTARLLGG
                     PTIEHYHQAIALVVAETFEQARAAASLVQAHYRRNKGAYSLADEKQAVSQPPEDTPDK
                     NVGDFDGAFSSAAVKIDATYTTPDQSHMAMEPHASMAVWDGNKLTLWTSNQMIDWCRT
                     DLAKTLKVPVENVRIISPYIGGGFGGKLFLRSDALLAALAARAVKRPVKVMLPRPSIP
                     NNTTHRPATLQHLRIGADQSGKITAISHESWSGNLPGGTPETAVQQSELLYAGANRHT
                     GLRLATLDLPEGNAMRAPGEAPGLMALEIAIDELAEKAGIDPVEFRILNDTQIDPADP
                     TRRFSRRQLIECLRTGADKFGWKQRNATPGQVRDGEWLVGHGVAAGFRNNLLEKSGAR
                     VHLEPNGTVTVETDMTDIGTGSYTILAQTAAEMLGVPLEQVAVHLGDSSFPVSAGSGG
                     QWGANTSTSGVYAACVKLREMIASAVGFDPEQSQFADGKITNGTRSAILHEATAGGRL
                     TAEESIEFGTLSKEYQQSTFAGHFVEVGVHSATGEVRVRRMLAVCAAGRILNPKTARS
                     QVIGAMTMGMGAALMEELAVDDRLGYFVNHDMAGYEVPVHADIPKQEVIFLDDTDPIS
                     SPMKAKGVGELGLCGVSAAIANAVYNATGIRVRDYPITLDKLLDKLPDVV"
     gene            14234..15190
                     /gene="paoD"
                     /locus_tag="N7B39_002446"
     CDS             14234..15190
                     /gene="paoD"
                     /locus_tag="N7B39_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor insertion chaperone PaoD"
                     /protein_id="HGU2422411.1"
                     /translation="MSYPLFDKDEHWHKPEQAFLTDDHRTILRFAVEALMSGKGAVLV
                     TLVEIRGGAARPLGAQMVVREDGRYCGFVSGGCVEAAAAFEALEMMGSGCDREIRYGE
                     GSPWFDIVLPCGGGITLTLHKLRSAQPLLAVLNRLEQRKPAGLRYDPQAQSLVCLPTQ
                     TRTGWNLNGFEVGFRPCVRLMIYGRSLEAQATASLAAATGYDSHIFDLFPASASAQID
                     TDTAVILLCHDLNRELPVLQAAREAKPFYLGALGSYRTHTLRLQKLHELGWSREETAQ
                     IRAPVGIFPKARNAHTLALSVLAEVASVRLHQEEDSCLPPSS"
     gene            complement(15369..16496)
                     /locus_tag="N7B39_002447"
     CDS             complement(15369..16496)
                     /locus_tag="N7B39_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2422412.1"
                     /translation="MTLCVFVLIASEFMPVSLLTPIARDLGVTEGLAGRGIAISGALA
                     VLTSLTLSTLAGKMNRKFLLLGMTVLMAVSGLIIALATSYLMYMVGRAMIGVAIGGFW
                     SMSAATAIRLVPQHQVTRALAIFNAGNALATVVAAPLGSYLGATVGWRGAFLCLVPMA
                     VVAFIWQCISLPSMDANKSHTSCGAVFRLFSRRMVVVGMIACGLFFMGQFALFTYVRP
                     FLESVTRVNSLGLSLILLIIGVAGFIGTLVVSIFLNRKFYPTLMAIPGLMAVIAIGLM
                     LTGHHVWMVSLLLGLWGMLATAAPTGWWTWIARTLPDNAEAGGGLMVAVIQLSIALGS
                     TAGGIVFDRLGWQSTFAMSSVLLLCAGVLTFVTARQKAGAL"
     gene            complement(16638..17696)
                     /locus_tag="N7B39_002448"
     CDS             complement(16638..17696)
                     /locus_tag="N7B39_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU2422413.1"
                     /translation="MSYADSTNPNAPVSMTDKWDKTFAESQKVDHRKVSFPNRYGITL
                     VGDLYLPKDRGDRKLAAIAVSGPFGAVKEQSSGLYAQTLAERGFVTLAFDPSYTGESG
                     GYPRNVASPDINTEDFSAAVDFLGLQKEVDRNRIGLLGICGWGGMALNDAAMDTRVKA
                     VATSVMYDMSRAMGHGVGDGEDRYSTADRRAVLQYLNEQRWKDAQSGTFAHGGHDINV
                     DSNGKVSAGERILPETLPANPHPVLKEFFDYYRMPRGFHERSVNSTGAWTATMPLSFM
                     NMPLLSYASEITIPTLIVTGEKAHSRYFAEDAYKAVGSKDKELVVVPGANHVDLYDNV
                     AGKIPFAKFEQFFQTKLK"
     gene            17942..18844
                     /locus_tag="N7B39_002449"
     CDS             17942..18844
                     /locus_tag="N7B39_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU2422414.1"
                     /translation="MKGNVSMAKRENYNELYLFMQVVREGSFTAAAKRLGLAQSGVSR
                     SVRELEERLGVQLLVRTTRKLSLTQAGEQLYQKTASGFEMLDLGLATLAHYRETPSGT
                     VRINASQHAIDKCLLPKLAVFKQRYPDIQLELINESRFVDIIEERFDAGVRLGPEVSQ
                     GMVAVRITPDMEMAIVGTPEHFRRYGFPQTPADLKAHPCIAYQFADGSVYQWELNQDD
                     KKITHQPEGQWAFSDSYMEAEAARLGLGLAYVPVELVADDLEHGKLIRVLQRYSLRME
                     GLFLYYPHRNVSPALRMVIDTLKI"
     gene            19081..19143
                     /locus_tag="N7B39_002450"
     CDS             19081..19143
                     /locus_tag="N7B39_002450"
                     /inference="COORDINATES: protein motif:HMM:NF041474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2422415.1"
                     /translation="MNTVVFVATVEYAYLSLHFY"
     gene            complement(19547..>19801)
                     /locus_tag="N7B39_002451"
     CDS             complement(19547..>19801)
                     /locus_tag="N7B39_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2422416.1"
                     /translation="LSIVREISVNTICSLIVVVALSLLSFSSVAKTITAVGSTINSTE
                     KEISLQAEKQGKSYKILGAFFKNRVYMIAKLTPVSKNDAS"
BASE COUNT         5040 a         5198 c         5059 g         4504 t
ORIGIN      
        1 tgtacgctta ccaatcactc atccaggcgt ttatgggagc gtcgccaatg gctgttaaca
       61 atgccagact tccccgttga ggaaattcca cataccacta atggtcacag ggattgggtg
      121 ttgaaatgat ccggatgagc atgtatcttt gtggttatgt tataacataa caggtaaaaa
      181 tgatgaagcc caatatccat cctgagtatc gtactgtggt gttccacgac accagtgttg
      241 atgagtactt taaaatcggc tcgactatca aaacagaccg tgagattgag ctggatggcg
      301 taacgtatcc gtacgtaaca attgatgtct cttcgaaatc gcacccgttc tatacaggga
      361 agctgagaac agtggcatca gaaggaaatg ttgcacgatt cacccaacgt tttggtcgtt
      421 ttgttagcac gaaaaagggg gcgtgatgaa agtccttaac tctctgcgta ccgcaaaaga
      481 acgccatcca gactgtcaga ttgtgaagcg aaaaggacgg ctatatgtga tttgtaaatc
      541 taatccacgc tttaaggccg ttcagggtcg taagaaaaaa cgttgattta aaattcgacg
      601 aattaacgat atttgtctga ttaataatca gaccggatta atgttggtgt atttattaca
      661 ccagcattaa ttttcctggg gatgtatctt cctgttcatt taaggtcaac tgcctgacgt
      721 ttctctccga atattccatt atattaatgt tgacttgttg accagcttcg cccctgtatg
      781 ctggcatcaa ccctctttta gactgaacac gccactcagt ttcctccctt tgcgacgcag
      841 cctgcattta cactcaaact gttaagatga taaatgtggt aaatctgttg gtactgacat
      901 aaaaacgttt acgccacagg aacggcctga tccaccggta accccgtcgc cgacgttcga
      961 gtgccagtta gagtaacgcg cacagatgac tgaatgcagt gccctggcaa agaggccatc
     1021 gttccggtga caactggcag tcttcgttta acttcactta atctggctct tagggggctt
     1081 accggacaga tgacgtactt acacctgttt aatttttcat cacttattgg gatgaacacc
     1141 cataaccatt ttgtgcggca tgttaatcca ttaaaacacc ttactgattg gcaaatcatc
     1201 tttaattatg actcatgata gtttcatatt ctatttctta tcatttagac ttgttttttt
     1261 actagtccat tacacaacac attaagacta ttcctaacac ctcagggcaa agttcctggc
     1321 taatataaaa tgcaagtaag aattgaacgt tatattgcca ataaccttat gaaactgaat
     1381 gtctttttct tcttatcaaa aaagcaatat tttcattttt tgtaaatatt gacttaacca
     1441 tggaattcat tttctgttca catattgaca ctcatcagga aaaaaacata aatttaaacc
     1501 taatcgaaat aattaaaaac ttaatctcgt ttaacctata ttgatatgtg ctacgtatct
     1561 tatttacttc cgatttacta aagaaactga atgtacctgt aaaaattaca ggtttggaaa
     1621 gtagtgacat ggcaaaatga ttacagcagg gactatgagg ttgaaaacca tatggaatgt
     1681 caaaaccgtt ctgataaata catctggtct ccccatgacg cctacttcta taaaggacta
     1741 tctgaactga ttgtggatat cgacagatta atttatctat cgctggagaa aatcagaaaa
     1801 gatttcgtgt ttatcaatct caatacggat tctttaactg aatttataaa ccgtgataat
     1861 gagtggttat ccgcggtaaa ggggaaacag gtcgtattga ttgcggccag aaagtcagaa
     1921 gccttagcaa attattggta ttacaacagc aatattaggg gcgtggtata cgctggactg
     1981 agtcgtgata ttagaaaaga actggcctat gtgattaatg gcaggttcct gagaaaagat
     2041 attaagaaag ataaaatcac tgaccgggaa atggaaatta tccgcatgac ggctcaggga
     2101 atgctgccta aatcgattgc cagaattgaa aattgtagtg taaagacagt gtatacccat
     2161 cggcgtaatg cagaggccaa gctgtactca aaattatata agttggttca gtaaactcca
     2221 ggcaagttag ttttaaaaaa tgactcactg ggacatcacg tcctcaattc aactcgggaa
     2281 gaaatacaat gaaaaaaaag gttctggcaa tagctctggt aacggtgttt accggcatgg
     2341 gtgtggcgca ggctgctgac gtaacagctc aggctgtagc gacctggtcg gcaacagcca
     2401 aaaaagacac caccagtaag ctggttgtga cgccactcgg tagcctggcg ttccagtatg
     2461 ccgaaggcat taaaggtttt aactcacaga aaggtctatt tgacgtggct atcgagggtg
     2521 actcaacggc taccgccttt aaactgacct cacgtcttat caccaacaca ttaacccagt
     2581 tggatacctc aggttccaca ctgaatgtgg gcgtggatta taacggcgcg gcagtcgaaa
     2641 aaactggcga taccgtgatg atcgataccg ccaacggcgt actgggcggc aaccttagcc
     2701 cgctggcaaa cggttacaat gccagcaatc gtaccaccgc acaggatggt ttcactttct
     2761 ccatcatcag cggcaccacc aatggtacca ccgcagtaac cgattacagc actctaccgg
     2821 aaggcatctg gagcggcgac gttagcgtac agttcgacgc gacctggacc agttaatctc
     2881 tctgatgtac cagcaggggt agccccccag ctttattcct ggacggaatt gttatgaaaa
     2941 agcaccttct gcttctcgct ctgctgttat ccggaatatc tccggcccag gcgctggatg
     3001 tcggcgatat atcatcgttt atgaacagtg acagcagcac gctgagcaaa acgatcaaaa
     3061 acagtaccga cagtggtcgc cttatcaata tccgtctcga acggctctct tcaccgcttg
     3121 acgacgggca ggttatctca atggacaagc cggacgagtt gctactcact cccgccagct
     3181 tgctgctacc cgcccaagcc agcgaagtga tccgcttctt ctataaggga ccagcagatg
     3241 aaaaagagcg ctactaccgc attgtctggt ttgatcaggc cctcagtgat gcgcagcgcg
     3301 ataatgccaa ccgcagcgct gtggccactg cttccgcccg catcggcacc attctggtcg
     3361 tcgccccccg ccaggcaaac taccactttc agtacgccaa cggcaccctg acaaatacag
     3421 gaaatgcgac gctgcggatc ctcgcctacg gcccctgcct gaaagccgcc aatggtaaag
     3481 agtgtaaaga gaattactac ctgatgccgg gcaagtcgcg tcgttttacc cgcgtggaca
     3541 cggcggataa caaaggacgg gttgcacttt ggcagggtga taagttcatt cccgtgaaat
     3601 agatagctgt gcagatggat aacgacaatg cctttacgac ggttctcccc aggactgaaa
     3661 gcccagtttg ccttcggcat ggtctttttg ttcgttcagc ccgatgccag cgctgctgac
     3721 ataagtgcgc agcaaatagg tggggtgatt attccgcagg ccttcagtca ggcgcttcag
     3781 gacggcatga gcgtcccgct ctatattcat ctcgccggta gccagggtcg ccaggacgat
     3841 cagcgaatcg gcagcgcttt tatctggttg gatgatggac agctacgcat ccggaaaata
     3901 cagctggaag agagtgaaga taacgccagt gtcagcgaac aaactcgaca gcagctgatg
     3961 gctctggcga acgccccgtt caatgaggcc cttaccatcc ccctgactga caacgcgcag
     4021 ctggatctca gcttgcgcca actgctgctg cagctggtgg tcaagcgcga agcgctgggc
     4081 actgtactac gctcacgtag cgaagacatc gggcagtcca gtgttaacac cctcagcagt
     4141 aatctgagct ataacttcgg catctataac aaccagttgc gtaacggcgg gagcaacaca
     4201 tccagctatc tgtcgctgaa taacgttact gcactgcgcg aacatcatgt ggtgctcgac
     4261 ggctcgctgt acgggatcgg tagcggtcaa caggacagtg aattatataa agcgatgtat
     4321 gaacgcgatt ttgccggtca ccgatttgcc ggtggaacgc tcgacacctg gaacttgcag
     4381 tccttagggc cgatgaccgc catttcagca gggaagattt acggcctttc ctggggaaac
     4441 caggccagct ccaccatctt cgacagcagc cagtcagcca cgccagtgat cgccttttta
     4501 ccggcggcgg gtgaagtaca tctcacccgt gatgggcggt tactaagcgt tcagaacttc
     4561 accatgggca atcatgaagt ggatacccgg ggtctaccat acggtattta cgatgtggaa
     4621 gttgaggtga tcgttaacgg tcgcgtgatc agcaaacgca cccagcgggt caataagctg
     4681 tttagccggg ggcgcggcgt cggtgcacca ctggcgtggc aggtatgggg cggtagcttt
     4741 catatggatc gctggtcgga aaacgggaaa aagacgcgac cagctaaaga gagttggctg
     4801 gcaggtgcct cgacctccgg ctcactgagt acgcttagct gggcggcaac gggatatgga
     4861 tacgataatc aggcggtggg tgaaacccgt ctgacgctgc cgcttggggg agcgatcaac
     4921 gttaacctgc aaaatatgct ggccagtgac agctcatgga gcagcatcgg cagcatcagc
     4981 gccactctac cgggaggctt tagttcgctg tgggttaatc aggaaaaaac ccgcattggc
     5041 aatcaattgc gacgtagcga tgccgacaac cgtgctatcg gcggcacact caacctgaac
     5101 tcactgtggt cgaagctggg cacattcagc atcagctaca atgatgaccg ccgttacaac
     5161 agccattatt acacggcaga ttactatcaa aatgtctaca gcggtacctt tggttcgctt
     5221 ggcctgcggg ccggtattca gcgctataac aacggcgaca gcaacgccaa tacagggaaa
     5281 tatatcgctc tcgatctctc gctaccactg ggcaactggt ttagcgcagg gatgactcat
     5341 caaaacggct acaccatggc aaacctgtca gcacgcaagc agtttgatga agggaccatt
     5401 cgcactgttg gtgccaatct gtcacgagcc atctccggcg ataccggtga tgacaaaact
     5461 ctcagtggtg gggcgtatgc acagttcgac gctcgctacg ccagcggaac gctgaacgtc
     5521 aatagcgcgg cggacggcta cgtcaatacc aatttaaccg ccaatggcag cgtcggctgg
     5581 cagggtaaaa acattgctgc cagcgggcgg actgatggca acgctggggt gatattcaac
     5641 accgggctgg aggacgacgg tcagatcagc gccaaaatca acgggcggat tttcccgctt
     5701 aacggcaagc gtaactatct cccgctctct ccctatggaa gatatgaggt ggagttacag
     5761 aacagcaaaa actcactcga cagttacgat atcgtcagcg gtcgcaaaag tcatctgact
     5821 ctctatccag gcaatgtcgc tgtcattgag ccagaggtga agcagatggt taccgtctcc
     5881 ggtcgtatcc gtgcggaaga cggcacactg ctggctaacg cacggattaa caaccatatc
     5941 ggccgaaccc gaaccgatga aaacggcgag tttgtcatgg acgtggataa gaaatacccc
     6001 actatcgatt ttcgctacag tggcaataaa acctgcgaag tggcactgga actcaaccag
     6061 gcgcgcggtg ccgtctgggt cggtgatgtg gtctgcagcg gcctctcatc gtgggcggcg
     6121 gtgacgcaga caggagaaga gaatgagagt taacctactg atagcgatga taatctttgc
     6181 gctaatctgg ccagtaactg cgctcagagc ggcagtgagc aaaacaacct gggcggatgc
     6241 accggcacgc gagtttgtgt ttgtcgaaaa caactcagac gacaactttt tcgtcactcc
     6301 tggcggggcg ctggatccgc gcctgaccgg tgccaaccgc tggaccggtt taaaatacaa
     6361 tggttcagga accatctatc agcaaagcct cggctacatt gataacggtt acaacaccgg
     6421 cctttatacc aactggaagt ttgatatgtg gctggaaaat tcaccagttt catctccttt
     6481 aactggcttg cgctgcatca actggtacgc tgggtgtaat atgaccacca gtcttatcct
     6541 gccgcaaacc accgacacca gtggatttta tggcgcgacg gtgaccagcg gcggcgcgaa
     6601 gtggatgcac ggcatgttgt cagacgcgtt ttaccagtat ctgcaacaaa tgcccgtcgg
     6661 cagcagcttt acaatgacca tcaatgcctg ccagacctct gtgaactatg acgccagcag
     6721 cggcgcacgc tgtaaggatc aggcctccgg caactggtat gttcgcaacg tcacccatac
     6781 gaaagcagca aatctacggt tgataaatac ccactcgctg gcggaagtat ttatcaacag
     6841 cgacggagta ccgactctgg gcgaagggaa cgccgactgc cggacgcaaa ccatcggcag
     6901 ccgttcagga ttaagttgta agatggttaa ctataccctg caaacaaacg gactcagcaa
     6961 cacctcaatc catatattcc cggcgatcgc caactcgtcg ttagcctcgg ccgtcggggc
     7021 gtacgatatg cagttcagtc tgaatggcag ttcatggaaa ccggtgagca atactgccta
     7081 ttactacacc ttcaacgaga tgaagagcgc agactcgatc tatgttttct tctcgagcaa
     7141 cttctttaag cagatggtga accagggaat cagcgatatc aacaccaaag atctattcaa
     7201 ctttcgcttt cagaacacca catcaccgga gtctggctgg tatgaatttt ctacctccaa
     7261 cacgctgatt atcaaacccc gtgatttcag catcagtatt atctccgatg aatatactca
     7321 gacaccgtcg cgggagggat atgtcggcag cggcgagtcg gcactcgatt tcggctatat
     7381 cgtaaccacc agcggtaaaa cagctgccga cgaagtgctg atcaaggtga ccggccccgc
     7441 gcaggtgatt ggcgggcgct cctattgtgt cttcagctcc gatgacggta aggcgaaagt
     7501 accgttcccg gcgacgcttt cctttattac ccgcaacgga gctacaaaaa cctacgatgc
     7561 cgggtgcgat gatagctggc gggatatgac cgatgcgctg tggttgacca caccgtggac
     7621 tgatatctct ggcgaagtgg ggcagatgga taagaccaca gtcaaatttt cgattccaat
     7681 ggataacgcc atttctctgc gtacggtaga tgataacggc tggtttggcg aagtcagcgc
     7741 ttcaggagaa attcatgttc aggcgacgtg gcgtaacatt aactaaggcc ctgctgacag
     7801 cggtctgtat gctggcggca cctttgacac aggcgatttc ggtcggcaat ctgacttttt
     7861 cactgccgtc cgagactgac tttgtcagca aacgtgtagt gaataacaac aaaagcgcgc
     7921 ggatataccg tattgccatc agtgctatcg atagcccggg cagcagtgaa ttacgcaccc
     7981 gaccggtgga tggtgaactg cttttcgccc cccgccagct ggcgttgcag gctggtgaga
     8041 gcgagtattt taaattttac tatcatgggc cacaggataa ccgcgagcgc tactaccggg
     8101 tctcatttcg cgaggtcccc actcgtaacc tgacaaaacg cagccctacc ggcggtgagg
     8161 ttagcacgga gccggtggtg gtgatggata ccattctggt agtacgacca cgtcaggttc
     8221 agtttaaatg gtccttcgat caggtgaccg gaacggtaag taacaccggc aacacgtggt
     8281 ttaagctact gattaaacca ggatgtgatt cgaccgaaga ggaaggcgat gcctggtatc
     8341 tacgtccgga agacgtggtt catcagcctg agttacgtca gccggggaat cactatctgg
     8401 tctataacga caaattcatt aagattagcg attcctgtcc ggctaagccc ccttcggcgg
     8461 actgatactt atccatcggt cgagggaaga attccacctc agagactcaa aaatcgttta
     8521 aatgatgtgg aagcgatcgt caatatcacc tgtaacgata ggctttctca tatgcacacc
     8581 acagtgttca ggagacacgg aaaggtacca ggcggcctgc taacgagtgg tttcttcact
     8641 aattaggacg taaaatcggt aacggctgga aatcattcaa tactcacact atcgaaagta
     8701 caccagccaa ccgcgatacg tccctgcata cgacgtaccg cagttctctt tacgatttat
     8761 agctgtactg gtgaattatg agcaatctga atccatgcat gacgtgtggt gcctgttgtg
     8821 catttttccg cgtctctttc tactgggccg aagccgacga tgctggcggc actattcccg
     8881 ccaggctcac tgaacaaata tccccttttc accgatgcat gagcggaact aatcagaaaa
     8941 acccccgatg tattgccctt gcaggaaccc cgggcaaaaa tgcctgctgc acgatatata
     9001 aaaatcgatc gtccacatgc agagaattcg ccatgtctgg tgagaacgga gtcgtcaatg
     9061 aggcttgcaa tcgtgcaagg gctaaatacg ggctgacacc gttataaata tacaataatt
     9121 atttgcactg ccccgccagc tgtaatagcg gggcttcgtt tttcaggggt aacaaacccc
     9181 gatacttctt ctatttgcca gcaacaatgc ctctcttctg tagcgttctt cggatctaca
     9241 tcatcctgaa gtagcgcgat atactcactg actatctgaa tcagaatata aaaagcaatg
     9301 tttttaacct ataaaaatgg cgctgtattt gcgccatttt tatcattcaa tgcattatct
     9361 gtttgagcct aaagggatct cagggtctgg ctcatgagta attctgtttc gtaaatctct
     9421 gcgaataatt tcaatggacc agaaccagac taaatgtcca aaaatttcag aaacattctc
     9481 ataccacggg agatcaaaca gaggtggtgt cagtcccatg agagggaatg aaatcatatg
     9541 aacaaaaagt tgggctaaag cacctgccag taagccctgc cagagtttaa tttttggaaa
     9601 tacttctgcg accacacaat aaccgacagc aaacactatc gaaaagataa tgtgcgtaac
     9661 accaacccag ttaaagacat gcccggcaaa ggtataaaca gccgcattgg gatctgtcag
     9721 ccccagccag tctcgaagaa aaatatacgg aggattgaga aaattacgcg agcaatcaat
     9781 ttgcccagca gccctgatta atgattccgg gccacacgct gcattaaaca tatccaccgg
     9841 gctacgtggc ggcaatggaa cttcagcccc ccacttcaca aatgcggaaa caacgccagc
     9901 aatcagccca atgaatgcag caagaccata acgtctgcgg ttcggtggag tttgttcaaa
     9961 tatattcata tctaccctgc ttgtaccatt atgttataca cctctttagg agtattcata
    10021 aaacaaggta aatgtaaaga actgtattgt tttgtataac aagatggttg tctaatcacc
    10081 aaagaatata agcaccatca tttctcccta tttttatatt agaagtgaca tagatttgca
    10141 gggtgatgca gagctgacaa tcacaggttt ccttatcggt ttttgcatcg tacaactaaa
    10201 gcaataaacc agctcccatc atcatcaaca tcccggcgaa catgaattta ttagccgggc
    10261 tcatagctgc attcgcgttt taagaataat cctcctgctg tcgccgacta tgcttaacgt
    10321 ttaaaaaagc atcagcactc tcgcaacgca ctcttatttt cccctttaga ataccggagg
    10381 cctggtatga gcaaccaagg cgaatacccc gaagataatc gggttgggaa gcatgagccg
    10441 cacgatttaa gtttgacccg tcgcgatctg attaaagtga gcgccgcaac ggcggcggcc
    10501 gccgtggttt atcctcattc tacgcttgcg gcaagcgttc cggcagctac acccgcgcct
    10561 gagataatgc ccctgacact taaggtgaac ggcaaaaccg agcagcttga ggtggatacc
    10621 cgaaccacgc tactggacgc tttgcgtgaa aatctgcatt tgatcggtac caagaaaggt
    10681 tgcgatcacg gacagtgcgg agcctgtacc gtgctggtca atggtcgcag gcttaatgcc
    10741 tgcctgacgc ttgcagtcat gcatcagggg gccgagatca ccaccattga aggcctgggt
    10801 tcaccagata atcttcaccc catgcaggcg gcctttatca agcatgatgg cttccagtgc
    10861 ggctactgca cctccgggca aatttgctca tcagtagcgg tgctaaaaga gattcaggac
    10921 ggcattccca gtcacgtcac ggtcgatttg gtttcccctc cagaaagaac tgccgatgag
    10981 atccgtgaac gtatgagcgg caacatctgt cgctgtggtg catacgctaa catccttgcc
    11041 gccattgaag acgctgcggg ggagataaaa tcatgaaggc gtttacctat gaacgagtga
    11101 acacccccgc cgaggcggca cttagcgctc agcgcgtacc cggcgcaaaa tttatcgcgg
    11161 gcgggaccaa tctgctggac ctgatgaagc tggaaattga aacgcccacc caccttatcg
    11221 atgtgaacgg gctcgggctc gataagatcg aagtgaccga cgcgggtggg ctgcgcatcg
    11281 gcgcactggt acggaacacc gacctggtgg ctcacgagcg cgtgcgtcgt gattacgcgg
    11341 tactctcccg cgccctgctc gctggcgcgt ctggtcagtt acgcaatcag gcaaccacag
    11401 caggtaatct gctccagcgc acgcgctgcc cctattttta cgacaccaat cagccctgca
    11461 ataagcgcct gcccgggagc ggctgcgcgg cgcttgaagg ctttagccgt cagcacgcgg
    11521 tggtaggcgt aagcgaagcc tgcattgcca cccatccgag cgatatggcg gtcgcaatgc
    11581 ggttgctgga tgcggtggtg gaaaccatca cgccggaggg aaagactcgc agtatcacac
    11641 tggctgattt ttatcaccca ccggggaaaa cgccgcacat tgaaaccgcc ctgcttcccg
    11701 gtgagcttat cgttgcggtg acgttacctc cgccgctcgg cggaaaacat atctaccgta
    11761 aggtgcgcga tcgcgcctcc tacgcctttg ccctggtatc ggtcgcggcg attattcagc
    11821 ctgacggcag cgggcgcgtc gcgctgggcg gagtagcaca taagccctgg cgcattgagg
    11881 ctgcggatgc tcagctatcc cagggggcgc aggccgtata tgacgcgctg ttcgccagcg
    11941 cccatcccac cgctgaaaac acctttaaac tcctgttggc gaagcgaacg cttgcctccg
    12001 tactggctga agcgagggca caagcatgaa atttgataaa cccgcagggg aaaacccgat
    12061 cgatcagctg aaggttgtcg gtcgtcccca tgaccgcatc gacggaccgc tgaaaactac
    12121 cggcacggca cgctacgcct acgaatggca tgaagaatcc cccaacgccg cctatggcta
    12181 tatcgtcggt tccgccattg ccaaaggacg cctcaccgcc cttgatacgg acgccgcgca
    12241 aaaagcgccg ggcgtactgc ctgtcattac cgccagtaac gccggggcac tcagtaaagg
    12301 cgacaaaaac accgccaggc tgttaggcgg ccccactatt gagcactatc atcaggccat
    12361 tgctctggta gtggccgaga ccttcgaaca ggcgcgagcg gcggcctcgc tggtgcaggc
    12421 gcactatcgc cgtaataaag gagcttactc cctggcggac gaaaaacagg ccgtcagtca
    12481 gccgccggaa gacacgcccg acaagaacgt cggtgacttt gacggggctt tctcctccgc
    12541 tgcggtgaag attgatgcta cctacacgac cccggaccag agccatatgg cgatggagcc
    12601 gcatgcctcg atggccgtct gggatggaaa taagcttact ctctggacct caaatcagat
    12661 gattgactgg tgccgcaccg atctggcaaa aacgctgaaa gtgcccgtgg agaatgtgcg
    12721 tattatctcc ccgtatatcg gcggagggtt tggcggcaag ctgttcctga gaagcgatgc
    12781 gctgctggcg gccctcgccg cccgagcggt gaaacgtccg gttaaagtga tgctcccccg
    12841 cccctctatt cccaataaca ccacgcaccg ccccgccacc cttcagcact tgcgtatcgg
    12901 tgccgaccag agcgggaaaa tcaccgctat ctcacatgaa agctggtctg gaaacctgcc
    12961 cggcggcacg ccggaaacgg cggtacagca aagcgaatta ctctacgccg gggcaaaccg
    13021 tcataccggc ctgcggctcg ccacgcttga tttgccggaa gggaacgcca tgcgtgcgcc
    13081 cggcgaagcc cccggtctga tggcgctcga aatcgcgatc gacgaactgg cggaaaaagc
    13141 gggcatcgat cccgtcgagt ttcgcatcct gaatgacact cagattgacc ccgccgaccc
    13201 gacgcgccgc ttctctcgcc gtcagcttat cgagtgcttg cgcaccggag cggataaatt
    13261 tggttggaag cagcgcaacg ccacacccgg acaggtgcgc gacggggagt ggctagtcgg
    13321 ccacggcgtc gcggcgggct ttcgcaataa tctgctggaa aaatcggggg ctcgggttca
    13381 cctcgaacca aacggcaccg ttaccgtgga aacggacatg accgacattg gcaccggcag
    13441 ctacaccatt ctggcccaga cggcagcgga aatgcttggc gtaccgctgg agcaggttgc
    13501 ggttcacctc ggcgattcca gtttcccggt ttctgcgggt tctggtggac aatggggcgc
    13561 gaatacctcc acctccggcg tttacgccgc ctgtgtgaag cttcgcgaaa tgattgcctc
    13621 ggcagtcggg tttgatcctg agcagtcgca gtttgccgac ggcaagatta ccaacggtac
    13681 ccgaagcgcc atactacatg aggccaccgc aggcggcaga ctgacagcgg aagagagcat
    13741 tgaattcgga acactgagca aggagtacca gcagtcgacc tttgccgggc attttgtgga
    13801 ggtcggcgtg catagcgcga cgggagaagt tcgggtccgg cgtatgctcg ctgtgtgtgc
    13861 tgcaggacgc atcctgaatc cgaaaactgc acgcagccaa gtcattggcg caatgactat
    13921 gggcatgggc gcggcactga tggaggagct ggcggtggat gaccgtttgg gctacttcgt
    13981 taatcacgat atggcggggt atgaggtgcc ggtccatgcg gatatcccaa aacaggaagt
    14041 gattttcctg gatgataccg accccatatc ctccccgatg aaggccaaag gtgtcggtga
    14101 gctgggcctg tgcggcgtga gcgcggctat cgccaacgcg gtgtataacg ccaccggtat
    14161 tcgggtacgc gattatccca tcactctgga taagctgctc gataagctgc cggatgtggt
    14221 ttaaggagga acaatgtcat acccgctttt tgataaagac gaacactggc ataagccaga
    14281 gcaggcgttt ctcaccgatg accaccggac cattctgcgc ttcgccgtag aggcgctaat
    14341 gtccggtaaa ggagcggtgc tggtgacgct ggtggagata cgcggcggcg cggcgcgccc
    14401 gctcggggcg cagatggtgg tgcgcgaaga tggtcgttac tgcggttttg tctctggcgg
    14461 ctgcgtggag gccgctgccg cttttgaagc gctggagatg atgggctcag gctgcgatcg
    14521 cgaaattcgc tatggcgaag gttcgccgtg gtttgacatc gttctgccct gcggcggtgg
    14581 gatcacgctg acgctccata aactacgctc ggcacagcct ctgctcgccg tgctgaaccg
    14641 cctggaacag agaaaaccgg cggggctgcg ctacgatccg caagcacaat cgttggtgtg
    14701 cctgcccacg caaacccgaa cgggctggaa tctcaatggc tttgaggtgg ggttcaggcc
    14761 atgcgtcagg ctgatgattt acggacgttc tcttgaggcg caggcaaccg cgagtcttgc
    14821 agcagccaca ggctatgaca gccatatctt cgatcttttt ccggcctcag ccagcgctca
    14881 gatcgatacc gatacggcgg tcattttgct gtgccatgat ctcaaccggg agctgccagt
    14941 gttgcaggcc gcgcgagaag caaaaccctt ttatctcggc gcattgggca gctatcgaac
    15001 ccacacgtta cgtctgcaaa agctccacga gctgggatgg tccagggagg agacagcgca
    15061 aatccgggca cccgtcggga tattccccaa agcccggaat gcgcatacac tggccctctc
    15121 cgtgctggca gaagtcgcct ctgtacgtct ccatcaggag gaggattcat gcctgccccc
    15181 gtcgtcctga tccttgcggc cgggcgtgga aagcgctttc tcacctccgg gggaaatacc
    15241 ataaattaag agaaaaattt gcagtagccg ttttagaaaa tcacctaaat cgtttaaaag
    15301 agcgccctac ccatagtggt tgctcatcgc cttgtgtaac tttttcagca acgcgcgccc
    15361 tcgccggact aaagcgcacc ggccttctgg cgtgctgtaa caaaggtcag tacgcccgcg
    15421 caaaggagca gcacactact catggcaaac gtactctgcc agccaaggcg gtcaaacacg
    15481 atgcctcctg ccgttgatcc aagggcgatt gagagctgaa tcaccgcgac catgagcccg
    15541 cctccggctt cggcgttgtc aggcaacgta cgcgcaatcc atgtccacca tccggttggc
    15601 gcggcggtgg ccagcatgcc ccagagtcct aacagcagcg agaccatcca gacgtgatgc
    15661 ccggtaagca tcagaccgat ggcaatgacg gccatcagtc cgggtatggc catcagcgtg
    15721 gggtagaatt ttctgttgag aaatatcgaa acaaccagcg tgccaataaa accggccacg
    15781 ccgataatca gcaaaatcaa cgacaagcct aaggaattaa cccgtgtcac cgactccaga
    15841 aatgggcgca cataggtgaa tagcgcaaac tgccccatga aaaacaggcc acaagctatc
    15901 atgccgacca caaccatgcg gcggctaaat aagcggaata ccgcgccaca tgacgtgtgg
    15961 cttttattcg catccatgct gggcagacta atgcactgcc agataaaggc gacaaccgcc
    16021 atgggcacca agcacaagaa agcgcctcgc catccgaccg tagcccccag atagcttccc
    16081 agcggcgcgg ccacgaccgt cgccagcgca ttaccggcgt tgaaaatcgc cagcgcacgc
    16141 gtgacctgat gctgcggcac cagacgaatc gccgttgccg ctgacatcga ccagaatccg
    16201 ccaatggcca caccaatcat cgcacgaccg accatataca tcagataact ggtagccagc
    16261 gcaataataa gccccgatac ggccatcaga accgtcatcc ccagcagcag gaacttacga
    16321 ttcatctttc cagctagcgt tgaaagcgtc aggctggtca gaaccgccag cgcaccggag
    16381 atagcaatcc cccgccctgc cagcccctcc gtcacgccca ggtcacgggc aatcggcgtg
    16441 agcaggctga cgggcataaa ttcagaagca atcagcacaa acacgcacag ggtcatggca
    16501 aaaataccgc cccagtatgc gtgctgatga tgaggtgatg gtgtttggct tagtgtcgac
    16561 atagtgaaat ttcacaaaga tcaggataaa agccaccggc catagaccgg tggctttgca
    16621 tcaattcagg ggaggtttta tttcagtttg gtctggaaaa attgttcgaa cttcgcgaaa
    16681 ggtatttttc cggccacgtt atcgtacagg tccacgtgat ttgcccctgg taccaccacc
    16741 aactctttgt ctttactgcc aaccgccttg taagcatctt cagcaaaata gcgtgaatgt
    16801 gctttctcac cggtcacaat cagcgtcgga atggtgattt cactggcgta gctcaacagc
    16861 ggcatattca taaatgacag cggcatcgtt gccgtccacg ccccggtaga gttaacggaa
    16921 cgttcgtgga agccgcgcgg catgcgatag tagtcaaaga actctttcag taccggatga
    16981 ggatttgccg gcagcgtttc tggcagaatg cgctcgcccg cactgacctt gccgttgcta
    17041 tcgacattaa tatcatgacc accgtgagcg aacgttccgc tctgcgcatc cttccagcgc
    17101 tgttcattca aatattgcag aacagcacga cggtcggcgg tagaataacg gtcttcgcca
    17161 tcccccacac catggcccat cgcccggctc atgtcataca tcacgctggt agcaaccgct
    17221 ttaacgcggg tatccatcgc cgcgtcgttc agcgccatgc cgccccagcc acaaataccg
    17281 agcagcccga tacggttgcg atctacctct ttttgcagcc ctaagaaatc caccgccgcg
    17341 ctgaaatctt cagtgttgat atccggtgag gcgacatttc gcggatagcc gccgctttcc
    17401 ccggtgtaag aaggatcgaa cgccagggta acaaaccctc gttccgccag cgtctgcgca
    17461 tacaagccgc tggattgctc tttcaccgcg ccaaatggcc cactgaccgc aatcgccgcc
    17521 agcttgcgat cgccacggtc tttaggcagg taaagatcgc ccactaaggt gatgccatat
    17581 cggttcggga acgagacttt acggtgatcg actttctggc tctcggcgaa cgttttatcc
    17641 catttatcgg tcatggacac aggggcatta ggattggttg aatcagcata actcattgtc
    17701 gtgactccac ttaatgatgc gaaaagcagc atgaccggca tcgcggtttt tagcgttcgg
    17761 gtgaaggctt tcataaaaat ccccataaaa aacaacagag agatacaggt ttccgacgga
    17821 caacgctgcg gtgctttact ttgatggggt cattatagtg agcagaatta gtgctgatta
    17881 gggggcgatt aatgctaaga ttaataccat attgttataa attaatgctg agattgccgg
    17941 catgaagggg aatgtttcaa tggcaaaaag ggagaactac aacgagctat acctgtttat
    18001 gcaggtggtg cgggaaggca gctttaccgc agcggctaag cggctgggtc ttgctcagtc
    18061 aggagtcagc cgttctgttc gcgagcttga agaaaggctg ggcgtccagc tgctggtgcg
    18121 caccacgcgt aaactgtcgc tgacgcaagc aggcgagcag ctctaccaga agacggcatc
    18181 tggatttgaa atgttagatc ttgggcttgc cacgctggcg cactatcgag agacgccctc
    18241 cggcacggtg cgtatcaatg ccagccagca cgcgattgat aaatgcctgc tgccaaagct
    18301 ggcggtattt aaacagcgat atcctgatat ccagcttgaa ctgataaatg agagccggtt
    18361 cgtcgatatc atcgaggaaa gattcgatgc aggtgtccgc ctggggcctg aagtcagcca
    18421 gggcatggtc gcggtacgca tcacgcccga tatggagatg gcaattgtgg ggactccgga
    18481 acattttcgt cgctatggtt ttccgcaaac cccggcggat ttaaaggcgc atccctgtat
    18541 cgcctatcag tttgccgacg gcagcgtata ccagtgggaa ctcaatcagg atgataaaaa
    18601 aatcactcat caacccgaag gacaatgggc cttttccgac agctatatgg aagcagaagc
    18661 cgcccggctg ggcctcgggt tggcctatgt tcctgttgaa ctggtcgcgg atgatttaga
    18721 acacggtaag cttatcagag tgttgcagcg ttacagcctg cgaatggagg gattgtttct
    18781 ctattaccct catcgcaacg tctcccccgc tctgcggatg gtcattgata cattgaaaat
    18841 ctgaacgtta acaacgaccg actcttcact gtgcaatatt gtgtcaggta gtcactctgc
    18901 gttcaattcc aggggccgct ttgagcgaga aagagcggac ttatctgtac aaggggacta
    18961 tccagcaatg gagatcatcc aaaagaaaaa accacctggc tcccctcgac aaccttaatc
    19021 cgggcattta gcctcaatca acattttcat atctgtcagg ttacttcatt atgaaaaccg
    19081 atcaataccg ttgtttttgt tgcaacagta gaatacgcgt atttatccct ccatttctat
    19141 taataacggc aataaactgt tcatttcact gatatttaaa atatgcatcc ttttcaagct
    19201 gttcagcggc gtaagaaaac gtattaaata tgaattcgac gatttaacca tttctgtctg
    19261 attggtaatc atctgtgatt aatgctggtc tattgattac tccagcatta accgcttagg
    19321 gatgtattct tcctgttgat atacagccct cgaaaaagag agaaaataaa actgtgtcca
    19381 atattactct accactccag accgaagcat cggtagaatt agcagaacga ctggtacaat
    19441 agattgggat agcgtcattt gtcaggtatt ccattgcctg acaaatgaca attttgagtc
    19501 aatagacgtg cttcttgatc atgtcttaaa aattatacca tgagccttat gaagcatcat
    19561 ttttactgac tggtgttaac tttgctatca tataaactct gttcttaaaa aacgcgccca
    19621 gaattttata tgatttccct tgtttttctg cctgtaaaga aatttctttt tcagtgctgt
    19681 taatggttga accgacggca gtaatcgttt tggcgacgct actgaatgat aataaagaca
    19741 gtgcaaccac aacaattaaa gaacagatag tattcacgct aatttccctg acaattgaga
    19801 g
//
LOCUS       DBFIFI010000071        19637 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.071, whole genome shotgun sequence.
ACCESSION   DBFIFI010000071 DBFIFI010000000
VERSION     DBFIFI010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19637
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.071"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            224..724
                     /gene="tssB"
                     /locus_tag="N7B39_002452"
     CDS             224..724
                     /gene="tssB"
                     /locus_tag="N7B39_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HGU2422417.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSSLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDEMNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GQVSNK"
     gene            759..983
                     /locus_tag="N7B39_002453"
     CDS             759..983
                     /locus_tag="N7B39_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2422418.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            1034..2425
                     /gene="tssC"
                     /locus_tag="N7B39_002454"
     CDS             1034..2425
                     /gene="tssC"
                     /locus_tag="N7B39_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HGU2422419.1"
                     /translation="MSLQEEELVSSHAGQPEQESSLLDQIMAQTRIQPGSEGYDVARQ
                     GVTAFIASILQSTASAEPVNKLAVDSMIADIDERISRQMDVIIHAPAFQQVESFWRSL
                     KTMVDRVDFRENIKVNVLHVTKQELLEDFEFAPEIIQSGFYKHVYSSGFGQFGGEPIA
                     AVLGAYEFKNTAPDMKLLQYVSAVGAMAHAPFLSSVSPEFMGLNSWTELPNIKDLYAI
                     FEGPAYTKWRALRDSEDSRYLGLTAPRFLLRQPYSPTDNPVKNFNYYEDVSQNHEDYL
                     WGNTAWMLACNIADSFAKYRWCPNIIGPQSGGAVKDLPVHLFETMGQIQAKIPTEVLV
                     TDRREFELAEEGFITLTMRKDSDNAAFFSANSVQKPKHFPGKDAETNYKLGTQLPYLF
                     IINRLAHYIKVLQREQLGSWKERSDLERELNTWIRQYVADQENPPADVRSRKPLRAAK
                     VEVMDVEGEPGWY"
     gene            2516..2929
                     /gene="tssE"
                     /locus_tag="N7B39_002455"
     CDS             2516..2929
                     /gene="tssE"
                     /locus_tag="N7B39_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HGU2422420.1"
                     /translation="MILDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            2933..4783
                     /gene="tssF"
                     /locus_tag="N7B39_002456"
     CDS             2933..4783
                     /gene="tssF"
                     /locus_tag="N7B39_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HGU2422421.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQMEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWSLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            4747..5829
                     /gene="tssG"
                     /locus_tag="N7B39_002457"
     CDS             4747..5829
                     /gene="tssG"
                     /locus_tag="N7B39_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HGU2422422.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLDHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTTFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            5854..7134
                     /gene="tagH"
                     /locus_tag="N7B39_002458"
     CDS             5854..7134
                     /gene="tagH"
                     /locus_tag="N7B39_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HGU2422423.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHAPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDHNDDISAAEMAQRHLAVTPLLRGLGGSLTVSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            7131..7655
                     /gene="tssJ"
                     /locus_tag="N7B39_002459"
     CDS             7131..7655
                     /gene="tssJ"
                     /locus_tag="N7B39_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HGU2422424.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLQAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPIKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7658..8989
                     /gene="tssK"
                     /locus_tag="N7B39_002460"
     CDS             7658..8989
                     /gene="tssK"
                     /locus_tag="N7B39_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HGU2422425.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGTLPDGTVFSIPDQDALPDPLHPQNFPDERSRNIY
                     LALPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDARTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHRARRSQLHPEDFYLDLAELLGELMTF
                     TEPSRLPCPLDVYDHHDLTKTFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADVH
                     DSELLQSATFVLAVAANMPVDQIQRQFIQQSKISSPEKIRNMVSVQIPGIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQAWTEMAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            8994..9755
                     /gene="icmH"
                     /locus_tag="N7B39_002461"
     CDS             8994..9755
                     /gene="icmH"
                     /locus_tag="N7B39_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HGU2422426.1"
                     /translation="MDEGSLSLPPFTGYDEKSLRDYHLALHGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWLCFSLGFR
                     GRYKVAVQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9764..12535
                     /gene="tssH"
                     /locus_tag="N7B39_002462"
                     /gene_synonym="clpV"
     CDS             9764..12535
                     /gene="tssH"
                     /locus_tag="N7B39_002462"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HGU2422427.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTLTDASDTLLARYAKNMTEDARNGRLAPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYERSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQTLVREIIALRQQLLGVAEDD
                     AAPLPDADTVEDTQPESESESEQDNTGAVPADETDREQPEETAETVSPVQRLAQLTAE
                     LDALHNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLPKWLGDTIKGQD
                     LAIASLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGRQYLTTI
                     NMSEFQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHPDVLNLF
                     YQAFDKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQEALYPVLADFFK
                     PALLARMEVVPYLPLSKETLATIIAGKLARLDNVLRSRFGAEVVIEPEVTDEIMSRVT
                     RAENGARMLESVIDGDMLPPLSLLLLQKMAANTAIARIRLSAVDGAFTADVEDAQNDE
                     SVTKDETVL"
     gene            12532..13275
                     /gene="vasI"
                     /locus_tag="N7B39_002463"
     CDS             12532..13275
                     /gene="vasI"
                     /locus_tag="N7B39_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HGU2422428.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDHLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADSRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            13280..14692
                     /gene="tssA"
                     /locus_tag="N7B39_002464"
     CDS             13280..14692
                     /gene="tssA"
                     /locus_tag="N7B39_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HGU2422429.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPDWEYIDGEIVK
                     LGSLAHAQLDIPELQRRGLQLLASESKDFRLLAHLLRTLQHAGDPLLALHLLTLYVEH
                     YWTVAAPQNMAHKKRFASQIIKRFETGIEGFSQNAATTQRDTLLGELAKLAQCWQSHN
                     VPELAQATDDLFALYQRTFNRAAPAPVPTPAASGSSPQTTVTSESGVTQPSAPAPQIA
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQSITSTPQAESDGRTPLAA
                     VSADMVADYQSRLASADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADTIR
                     DEVIRFLERLPQLTGLLFNDRTPFLSEQTKQWLAASPDGKVAPVAQIGEESQAARACF
                     AGQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLIQLAQQQYRMLLMIGSQMM
                     VSDWEPSLLTQLEQKFTAEQ"
     gene            14829..18263
                     /gene="tssM"
                     /locus_tag="N7B39_002465"
     CDS             14829..18263
                     /gene="tssM"
                     /locus_tag="N7B39_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HGU2422430.1"
                     /translation="MLILAWIFLLVWIWWKGPTWTLYEEQWLKPLANRWLATAAWGII
                     ALVWLTVRVMKRLQLLEKQQKQQREEAIDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGARGTEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHALGWLKEKRARQPLNGIILTLDLPDLLTADKRHREHLL
                     QTLRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVTQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGVYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     SLFPQALLAEPNLATESRAWLMRSRRRLTVFSATGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPPQGEDDYGNLQLPLLNPVRDATLAYGDWGDRSRLADMGLYQGRR
                     IGPYVEQTYLQLLEQRYLPSLFNGLVKAMNAAPPESEEKLAVLRVMRMLEDKSGRNNE
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWTPYDKP
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFLTRYGLQSYFVKQRDELVELTAMDSWVLNLTRSVKYSDADRAEIQRQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLQRALTVLRDNTQPGVFSEKLSA
                     KERDEALAEPDYQLLTRLGHEFAPENITLAVQKDKESTMQAVYQQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGRLTDQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNEQLANNYPFNPRSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNNVIIREDIIAQLETAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGNAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGNFTVRFSVDGGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            18274..>19637
                     /locus_tag="N7B39_002466"
     CDS             18274..>19637
                     /locus_tag="N7B39_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
                     /protein_id="HGU2422431.1"
                     /translation="MNSNVLTQTIVTGSDPRGLPEFSAIREEINKASHPSQPELNWKL
                     VESLALAIFKANGVDLHTATYYTLARTRTQGLAGFCEGAELLAAMVSHDWDKFWPQGG
                     PARTEMLDWFNSRTGNILRQQISFAESDLPLIYRTERALQLICDKLQQVELKRVPRVE
                     NLLYFMQNTRKRLEPQLKSNTENAAQTTVRTLIYAPETQASSTPEAVVPPLPGLPEMK
                     VEVRSLTENPPQASVIKQGSTVRGFIAGIACSVAVASALWWWQVYPVQQQLLQVNDTA
                     QGAATVWMASPELENYERRLQQLLDTSPVQPLETGMQMMRVADSRWPESLQQQQASTQ
                     WNEALKTRAQSSPQLRGWLQTRQDLHAFADLVMQREKEGLTLSYIKNVIWQAERGLGQ
                     ETPVESLLTQYQDARAQKQNTDALEKQINERLEGVLSRWLLLKNNTIPTIKKALNFNN
                     IHEY"
BASE COUNT         4573 a         5436 c         5465 g         4163 t
ORIGIN      
        1 gcgaattgca caggttcata aaatctattt aacgcttgca gaatacccct ctttacgatc
       61 gataaaaagt ccttttctta tttattttga agcgtaaaat gcggggtgat gaaggagtgg
      121 tgattttatt ttcaccgcca tcaagacggc atttccgata ttttttgcgg tacgaatatg
      181 ccgtgttcat ggcctgtacg agataacatg acggatcact tttatgagca aaaaatttga
      241 aggcagcgtc gcgcctcgcg aacgtattaa tatatcgtat gtcccaaaaa cagatggcca
      301 gacggctgaa gtggaacttc cgcttaacat gctcgttgtc ggcgataccg gaaatacgca
      361 ggaaacatcc tcactggatg aacgtcaggc agtttccgtt aacaagcaca atttcggtgc
      421 agtgatggct gaagcagcca ttggcctcaa ttttaccgtc cccgccacgc tgaagggaag
      481 cacaacggac gatgaaatga atgtggcgct gaatatcaag tcactggatg atttttcacc
      541 agatagcgtg gcccggcagg ttccggaagt gaataaattg ctggaactgc gtgaagcact
      601 gactgcactg aaggggccga tgggtaacct tccggcattc cgtacccagc tccaggcatt
      661 gctggaaaat gaagagtccc gcgagcagtt gctcaaagaa atcggccagg tgagcaataa
      721 ataaccacca ctatttcatt aaacagggtt atccaggcat gacggcacac aaaaatatct
      781 ccgggacata ccacctctct gttgcagaca ttctgcaggt ggtttaccag gtatgtttca
      841 gcccgtccgt tgaaatcaac caggatggtg tggctgcgct gattacaaca ctggatcgcc
      901 gtatcagtga tctattggat gagattatcc atttttgtga gtttcagcag agcgcgtcgc
      961 actggcagcg cgtacttcac tgacggcgaa aagccggttt cagaaacaca gctaaaacag
     1021 aaggattttc agtatgtctt tacaggaaga ggaactcgtt tccagtcatg ccgggcagcc
     1081 tgagcaggaa tcgtcgctgc ttgatcaaat catggcacag acccgtattc agcccggttc
     1141 ggaaggttat gacgttgccc gccagggtgt gacagccttt attgccagca tcctgcaatc
     1201 cactgcatct gccgaaccgg tcaataaact ggccgtggac agcatgattg cagatattga
     1261 tgaacgcatc agccgccaga tggatgtcat catccatgcg cctgcctttc agcaggtcga
     1321 gtcgttctgg cgctcactga aaaccatggt ggatcgcgtt gatttccgtg aaaacatcaa
     1381 ggtcaacgtg cttcatgtca ccaagcagga acttctggaa gactttgagt ttgcgccaga
     1441 aattattcag tccggatttt ataagcacgt ttattcgtcc ggtttcggac agtttggcgg
     1501 tgaacccatc gctgccgtgc tgggcgctta tgagttcaaa aataccgcgc ctgacatgaa
     1561 actgctgcaa tacgtcagtg ccgtgggcgc aatggcgcac gcaccgttcc tgtcctccgt
     1621 ttccccggag tttatggggc tgaactcgtg gaccgaactg cctaatatca aagatcttta
     1681 tgccatcttt gaaggtccgg cctacaccaa atggcgcgcc ctgcgtgact cggaagattc
     1741 gcgctattta gggcttacgg caccccgctt cctgcttcgc cagccctatt caccaacaga
     1801 taaccctgtt aagaacttta actattacga agatgtcagc cagaaccacg aagattatct
     1861 gtggggtaat acggcctgga tgctggcatg caatatcgca gacagttttg ccaaataccg
     1921 ctggtgtcca aacattatcg gtccgcaaag cggcggcgca gtgaaagatc tgccggtgca
     1981 tctgttcgaa acgatggggc agattcaggc caaaatccca accgaggtgc tggtcaccga
     2041 tcgccgcgaa tttgaactgg ctgaagaggg ttttatcacc cttaccatgc gtaaagactc
     2101 tgataatgca gcctttttct ctgcaaactc ggtacaaaaa ccgaagcact ttccgggaaa
     2161 agatgcagaa accaactata agctgggtac gcagcttcct tatctcttca tcatcaatcg
     2221 gttagcgcac tacatcaagg tgttgcagcg tgaacaactg gggtcatgga aagaaaggag
     2281 cgacttagaa agagagctta atacctggat ccgtcagtat gttgccgacc aggaaaaccc
     2341 gcctgcagac gtgcgcagcc gcaaacctct gcgcgctgcc aaagttgagg ttatggatgt
     2401 agaaggcgaa cccggctggt actaggttgc gctaagcgtg agacctcatt ttaaatttat
     2461 gggggcaaat tttgagcttt ccctggttgg ccggttagac agggagtaac acaccatgat
     2521 actggatcgg gaaagaacca caggttctct ttttgagcgt atggaagcct cttcggccag
     2581 aaaccggcag ggaggcagta ttcattcgct caggcaatcc attcgccaga acctaagaaa
     2641 cattcttaat acccggtccg gtagttgccg gggtgcgcct gagctgggca ttgatgaacc
     2701 ggaaggggct gaaaacttcc gcgaatccat gagtcgggcc atcgagcaat gtatagaacg
     2761 ctatgaaccc cggatctcac acgctgaagt gcaggcagtg gtttcatccg cctccagccc
     2821 gctggatatg acgtttcata ttacagcctg ggtgacattt aacgaaacgc atgaggtgct
     2881 tgaatttgat atggcaccga atggcagcca gcattaccgg gtggactgac aaatggaatt
     2941 tgaagaacgc tattttcggg aagaactcga ttacctgcgc cagcttagca agctgctggc
     3001 aacggaaaaa ccccatctgg cccgcttcct ggccgaaaaa gatgcggatc cggatattga
     3061 acgcctgctg gaaggggtgg cttttcttac cggcaatctc cgccagaaaa ttgaggatga
     3121 attcccagaa ctgacgcacg ggcttattaa gatgctatgg cctaattacc tgcgtccggt
     3181 tccggcaatg acccttattg aatatacgcc ggatatggat aagtcttctg taccggtgtt
     3241 aatcccccgt aatgagcagt ttacaaccaa cgccggggaa atcagagttg atgaagtgct
     3301 gccctctgat gctaaaaagg aggagccgcc tccctgtacc ttcaccctct gccgggatat
     3361 ctggctgctg cccgttcgcc tggagcagat tgaaaaccgc agtacgaccc gtaatggtgt
     3421 tatcaacatc accttttcgg tcgcaccggg aacggacttc cgcacgctgg atctgaacaa
     3481 acttcgcttc tggctcggca atgacgacaa ctatacccgt gaccagcttt atttatggtt
     3541 ctgcgaatac ttgcagggtg ccgacctgac tgtgggtgaa cagcatattc gcctgcctga
     3601 gtttatgcta aaagctgtcg gttttgagcc gcaggatgcc atgctgccct ggccgaaaaa
     3661 cgtccacagc ggctaccgga tccttcagga gtatttctgt taccccgatg cgtttctctt
     3721 ttttgatctt tgtggttgtc cggctttgcc tgacggattg caggcggaat tctttaccct
     3781 gcaactgcgt ttttcgcgcc ctttgcccgt ggacatccgg ctgcgccgcg attccctgcg
     3841 cctgtattgc gcacctgcca ttaatttatt tatccaccat gcagaagcca tcacgctgga
     3901 caaccggcgg gcagactatc cgctggttcc cagccgccat tacccacaac attacgatgt
     3961 attttccgtt aacagtgtgg tgagccaggt ccaggatatg ttcaggaaaa aagatctggg
     4021 gcgtcctgtt tcgacgcagg ccgcgcgcca gtggccagcc tttgaaagtt tcagccatca
     4081 gatggaatac agccggaagc gggaggtggt gtactggcat caccggacca aaacatccct
     4141 gttccatcgc ggctttgatc atacccttgc ctttatacat gctgatggca gttatccgtc
     4201 agacgaatct ctgctcagta atgaagtggt ttcggtatcg ctgacctgta ccaaccgtga
     4261 gcttccgtca caaattcgtt ccggcgatat caccggcaca accggtaaaa atgcggctgt
     4321 tgcttccttt cgcaacatta cccgcccgac gcaaccactc tggccggtca ttgatggcag
     4381 cctgcactgg tccctgctct ccgccatgaa cctgaattat ctgtcattac tggatacgga
     4441 cgcgctgaag caggtcatcg ccaactttga tcgccacgca atccaccatc cgcagacggc
     4501 gcggctgtca caacaaaagc tggatgccat tgagcgtctg gagacccgcc ccgttgatcg
     4561 cctgtttacg ggtattcccg tccggggact ggcctccacg ctgtatctgc acccggagcc
     4621 gtttgtctgt gaaggggaaa tgtatctgct cggtacggtg ctttcgcatt ttctgtcgct
     4681 gtacgccagc gttaactcat tccacatgct gaccgttgtg aacacagaaa gccaggagac
     4741 atggaaatgg acggaaagaa tcgggcagca tcctcttatc tgagtccggg aaatcccccg
     4801 gcagataaag agcagaatga tccgctggca caggttttcc ataatgcctg ctcttacaat
     4861 ttttttgcga tggcggagct gttgcaccgc ctggcaaagg gtgaaaaggg gacgccagaa
     4921 ttatccctgc gcgacgatcc ggcacaggaa accctgcgtt tcagcgccga tgccagcctt
     4981 gccttcccgt gcagtgatat cagtgcgctg aaacgggata cgtcaggggc attcaggatg
     5041 accactacct ttatggggtt acaggggagc cagtcgcctc tccccggtta ttacctcgat
     5101 cacctggcct ggaaagcggt acatgaacaa agtccggtag gtgattttct ggatatgttc
     5161 agccaccgcc tgacccagtt tgtctggcat atctggcgta agtaccgcta tcacatcagt
     5221 tttcgtaacg gcggtgtgga tgcgttctcg cagcgcatgt actcccttgt cgggctgggc
     5281 caccgccagc ttcgcgataa gctggcgatt aatcacagca aaatgctggc gtattccggg
     5341 atcctggcaa atccgggacg atccccggaa attatctgtg gcctggtgtc ccactgcttt
     5401 gatttgtcag aagtgacgct gcaaaactgg caacgtcgca aggtggatat tgaaccggat
     5461 cagcaaaaca gcctggggag ctattctctt aaaaatggcg aaaaactggc gggaagatcg
     5521 gtactgggca actttgttct gggtacacga gtacccgatc tcagcggaaa attccagctc
     5581 agtattacca gcctgacccg aaaacagttc ctctcttttc tgccgtccgg cgaaaacttc
     5641 ctgccactga cgacgttcgt gtccttcatc ctgcgcgatc agcttgcctg ggacttacat
     5701 ctggggctgg cgccggagca ggtgggcgca atgcgcctgg gcgataacaa aagtgcgctg
     5761 ctgggctgga ccagcttcct cggcacaccg gaagaacgac cctcagtcac catcagggta
     5821 cggtcgtaaa ttatgataat cacggagcag aatatgccgg aagagaaact acagacgctt
     5881 tcattgcagg tcattaacgg cagtgagctg gaaagcgggc gggcggcgcg ctgtctgttc
     5941 acacagcagg gaaatgtcgg ccatgccccc gaatgccact ggtcggtaca ggatcgtcag
     6001 cagagcattc cggcccaggc ttttaccgtt atcctgcacg atggcacatt ttgtctacgc
     6061 ccgcagacgg cacaactgtg gctgaatcag gcaaaagtca cagcaacatc agacctgata
     6121 cagttgcgcc agggcgatga gatccagatc ggacggctga tggtgagggt tcatctgaac
     6181 cggggagata ttccccatta cgatgaggaa atggccactc ccgaaaccat cgttaccaat
     6241 cgcgatatgc tcacggatac cctgctatca acggagggtg cgccacacta tccgggaatg
     6301 actcaccggc accagcttgc agacaccgtg gtaaatggtt tttctgccga tccactccag
     6361 gcacttcagt ccgaaagcct gattaccacg ggcgatccgc tttcaggcat tgcggctgtc
     6421 cggccatcgg caccgctgtc cgatccggca agtaatgggg ggatcaatac tccgtttatg
     6481 gatctgccgc ccatttatgc cagccctggc gatcataatg atgacatctc tgcggcagaa
     6541 atggcgcaac gccaccttgc ggtcaccccc ttactgcgcg gtcttggcgg ctcgcttacc
     6601 gtgagcaatt ccgacgatgc ggatgatttt ctggaggagg ccggacgaac gttacaggcc
     6661 gcaataaaag gtctgctcga tttgcagcag cagcgtaaca gcctctcaga caaacatttg
     6721 cgcccgctgg aagataaccc gctgcgcctg aacatggatt acgccaccgc gctcgacgtg
     6781 atgtttgccg aaggtaaaag cccggtacat ctggcggctc ccgccgccgt cagtgaaagc
     6841 ctgcgcaatg tccgccacca cgaagaagct aaccgggcgg cgattgtgga gtcgcttcgt
     6901 gtcctgctgg atgctttctc accacaaaat ctgctgcgcc gctttgtgca gtaccgccgc
     6961 agccatgaac tgcgccagcc gctggatgat gccggagcat ggcaaatgta cagccattat
     7021 tacgaagaac tggcctccga tcgccagcag gggtttgaga tgctgtttaa cgaggtctac
     7081 gcccaggtct atgaccgggt gcttcgtgaa aaacagcggg agccggaagc atgaacagga
     7141 aggcgtttct ggcctgcgtg ctgatgtgcg tcatattaac cggctgcgaa acggcgaaaa
     7201 aaatcagcga ggtgatcaaa aacccggata tccaggtcgg gagcctgaag gagcaacctt
     7261 cggaaataac cgtcaccctg ctgactgaac cggacaccaa taccaatgcg gagggtgaat
     7321 cagccgccgt ggatgtgcaa ctggtgtacc tgactgacga ctcaaaattg caggcagccg
     7381 actatgacca gatagccagt acgccgctac cggatgtgct gggtaaaaac tatatcgacc
     7441 accaggattt caacctgttg ccggacacca tcaaaaccct gccgccgata aagctggatg
     7501 agaaaacgca gtttattggt gtcgtggcct atttctccga tgaccaggcc actgaatgga
     7561 aacaaatcga gacggtggaa ggaaccggcc accattaccg cctgctggtg catgtacgtc
     7621 aaagcagcat cgaaatgaaa aaagaggatg aataattatg gcaaccaccc gcaacaaggt
     7681 gatgtggcag gaagggatgc tgatgcgccc acaccatttc cagcaacagc agcgttacaa
     7741 cgactacctg gataaccagc gtttccgggc catgaatgat ttatcctggg gatttaccga
     7801 actcaccctc aacaatgaac tgctggcgca gggtaagatc atgattgaca gcgcgtcagg
     7861 cacactgcct gacggcaccg tcttttctat ccccgaccag gacgcactgc ccgatccgct
     7921 gcacccgcaa aattttccgg acgaaagaag ccgcaatatt tacctcgctc tgccggtcgc
     7981 cagtgatgtg agaaatgaaa tcagcgacgg gcggcgaatc gggcgttacc ggctgaatta
     8041 tgccgatgtc cgggatctgc attcagaaga aggcgacgcg cgaacactga cgctgggaca
     8101 actgacgccg cgcattatga gcggtgcaga agatatgagt gcctatatta cgctcccgct
     8161 ttgccgtatc agcgatcgcc atgccgacgg ctccctgacg ctggatgacg attttatccc
     8221 ctcctgccag aatattcagg tcagtaagaa actgcgtgtt tatctcaaag aggtacaggg
     8281 ggccattggc ggacgggcaa gcgatctggc aaaccgtatt ggctctccgg cgcagagcgg
     8341 catcgcggat gtggcggaat ttatgatgtt gcagttgctt aaccgtaacc agacccggtt
     8401 tacccatcgc gcccgccgat cccagctcca cccggaagat ttctaccttg atcttgccga
     8461 gttgctgggt gaactgatga cctttacaga gccgtcacgc ctgccctgcc cgcttgatgt
     8521 gtatgatcat catgacctga ccaaaacatt taaaacactg ttaccggaag tcaaacgggc
     8581 gctgcatacc gtactgtcgc caagagcggt caatctgccg ctgcatctgc gtgacggcat
     8641 ctggcaggcc gatgttcatg actcggaact gctacagtct gccacctttg tactggccgt
     8701 ggcagcaaat atgcctgtcg atcagatcca gcgtcagttt atccagcagt cgaaaatttc
     8761 ctcgccggaa aaaatccgca atatggtcag tgtgcagatc ccgggtattc ctctgcgcgc
     8821 actgatggtg gccccccgcc agcttcccta ccattccggg ttcagctatt tcgaactcga
     8881 caagagcggc caggcctgga cagaaatggc tgccgccggg gccgttgcac tgcatgtatc
     8941 cggcagtttc ccggatctga acatgcaact gtgggcgata agagggtaag acgatggatg
     9001 aggggtcact gtctctgcca ccatttaccg gatacgacga aaaaagcctg cgcgactacc
     9061 atctcgcgct tcatggcaac agcctgaatc cgatgatcga cgcggcgacg ccgctactcg
     9121 gtatggtaat gcgcctttcc accatgaaca gccagacgat gccggagcat ctttttgctc
     9181 aggtggtgac agatgttcag gccgtggagc aactgcttca ggaacagggc tacgaaccgg
     9241 gcgtgattat ctcgtttcgc tacatcctct gcacgtttat tgatgaagcg gcgctcggta
     9301 acggctggtc gaacaagaac gagtggatca agcagtcatt gctggtccat ttccacaacg
     9361 aagcctgggg tggcgagaaa gttttcatcc tgctggagcg cctgatccgt gaacccaaac
     9421 gttaccagga tctgctggaa tttctctggc tctgtttttc tctgggattt cgcgggcgct
     9481 acaaagtggc agtacaggat cagggggaat ttgagcagat ctaccgccgt ctgtaccacg
     9541 ttctgcataa actgcgcggc gatgcccctt tcccgctgtt acaccaggac aaaaaaaccc
     9601 aaggcgggcg ttatcagctc atcagtcgcc tgactgttaa acatattttt tgtggtggcg
     9661 ttgtcgtgct ggcgttgttt tacctgttct atctgttgcg gctggacagc cagacgcagg
     9721 acatcctgca ccagctaaac aaattacttc gataaggacg tttatgatcc agattgatct
     9781 tcccacgctg gtaaaacggc tgaacctgtt ctcccgccag gcgctggaga tggccgcctc
     9841 agaatgtatg agccagcagg cagcggaaat taccgtcagc catgtgctta ttcagatgct
     9901 cgccatgcca cgcagtgacc tgcgggttat tacccgccag ggcgatattg gcatggaaga
     9961 gttgcgccag gcgttgacgg tagagaacta cacaaccgcc cgttctgcgg acagctaccc
    10021 ggcgttttcc ccgatgctgg ttgagtggct taaagagggc tggctgctgg cgtcggctga
    10081 gatgcagcac agcgaactgc gcggcggcgt gttgctgctg gccttgctgc attcgccgct
    10141 gcgttatata ccgcctgctg ccgcccggct gttgaccggc attaaccgtg accgtctgca
    10201 acaggacttt gtgcagtgga cacaggagtc ggcggaatca gtcgtgccgg atgcagacgg
    10261 taaaggcgca ggcacactga cggacgcctc tgacaccctg cttgcccgct acgccaaaaa
    10321 catgacagaa gacgcccgta acggcaggct agccccagta ctgtgccgcg accacgaaat
    10381 cgacctgatg atcgacattc tctgccgccg ccgtaaaaac aacccggtgg tggtgggcga
    10441 agcaggcgtg ggtaaaagcg cgctgattga agggctggcg ctgcgcatcg tggcaggcca
    10501 ggtgccggac aaactgaaaa acaccgatat catgaccctt gacctgggcg cattgcaggc
    10561 cggggcatcg gtgaagggtg agtttgaaaa acgcttcaag gggctgatgg cggaggtcat
    10621 ttcctctccg gtgccggtca ttctgtttat cgacgaagcg cataccctga ttggcgcggg
    10681 caaccagcag ggcgggctgg atatctccaa cctgctgaaa ccggcgctgg cgcgtggcga
    10741 actgaaaacc atcgccgcca ccacctggag cgagtacaaa aaatacttcg agaaagacgc
    10801 cgccctgtcg cgccgcttcc agttagtgaa ggtcagcgaa cccaacgctg ccgaagccac
    10861 cattattctg cgtggtctgt cggcggtcta tgaacggtct cacggcgtgc tgattgacga
    10921 tgatgccttg caggccgcag caacgctgag cgaacgttat ctctccgggc gtcagttacc
    10981 ggacaaagcg attgatgtgc tggataccgc ctgcgcccgt gtggccatca acctgtcgtc
    11041 gccgccgaag caaatctcgg cgctgaccac tctgagccac cagcaggagg cggaaattcg
    11101 ccagcttgag cgcgagcttc gcatcggact gcgtaccgac acatcacgga tgaccgaggt
    11161 gctggtgcag tatgatgaaa cgctgacggc gctggatgaa ctggaagcgg cttggcacca
    11221 gcagcagacg ctggtccggg agattatcgc gctgcgccag cagttactgg gcgtggcaga
    11281 ggacgatgcg gcgccgttgc cggacgcaga tactgtggag gatacgcagc cagagtcaga
    11341 gtcagagtca gaacaggata ataccggtgc cgtaccggct gatgagaccg acagagaaca
    11401 gccggaagag accgctgaaa cagtttcccc ggtacagcgg ctggcccagc tcactgccga
    11461 actggacgcc ctgcataacg accggttgct ggtctccccg cacgtcgata aaaaacagat
    11521 tgcggcggtg attgccgaat ggaccggcgt gccgctcaac cgcctgtcgc agaatgaaat
    11581 gtcggtcatc accgacctgc cgaaatggct gggcgacacc atcaaaggcc aggacctggc
    11641 gattgccagc ctgcataagc atctactgac cgcacgcgcc gacctgcgtc gtccgggacg
    11701 cccgctcggt gcgttcctgc tcgctggccc cagcggcgtg ggtaaaaccg aaaccgtcct
    11761 gcaactggca gagctgctct acggcggtcg ccagtacctg accaccatca atatgtccga
    11821 gttccaggag aaacacaccg tttcgcggct gattggctcc cctccgggct atgtcggcta
    11881 tggcgaaggc ggcgtgctga ctgaagccat ccgccagaaa ccctactcgg tggtgctgct
    11941 cgatgaagtg gaaaaagcgc acccggatgt cctcaacctg ttctaccagg cgtttgacaa
    12001 gggcgagatg gccgacggcg aaggccgcct gattgactgt aaaaacatcg tcttcttcct
    12061 cacctccaac ctcggttacc aggtgattgt cgaacacgcg gatgacccgg aaaccatgca
    12121 ggaagccctc tatccggtgc tggcggactt ctttaaacct gccctgctgg cgcgaatgga
    12181 ggtggtgccg tacctgccgc tgtcgaaaga gacgctcgcc accattatcg ccgggaaact
    12241 ggcccgtctg gataacgtgc tgcgcagtcg ctttggtgca gaagtggtca ttgaaccgga
    12301 agtgacggac gaaatcatga gccgcgtcac ccgcgcggaa aacggcgcga ggatgctgga
    12361 atctgtcatc gatggcgaca tgctaccgcc gctctcgctg ctgctgttgc agaaaatggc
    12421 ggctaacacg gcgattgccc ggattcggtt gtcggcagtg gacggcgcat ttacggcaga
    12481 cgtggaagat gctcagaacg acgagtccgt cacaaaggat gaaacggttt tatgagcagg
    12541 ttgcgcgttt tccggcagac aggatggttt atcgcgggcc tgatgacggg cttacccgcc
    12601 actgccgcgc ccgcagaagc atcgtcaatg gccggtgtgg cggtcgccgt cgccaccacg
    12661 accccgccgg atgcgacagc cacgttacag gccatgcagt cctgtcggcg ggagtccgcg
    12721 gcgctggagc ggcttgactg ctatgaccac ctgctggcac cgctgtcccc gtcagggttt
    12781 gacggggcgc tggtcaaagc cggttttgtg ggcgaggcct ggacccgcgc cacagagcag
    12841 gaaaaacgcc gcgagggcaa caccacagaa ctgctggtga cgcaggtgcc gggggagcgc
    12901 ccgacggtgg tgataaccac cccggctatc ggccatgttc cgccgcgccc ggtgctgatg
    12961 ttcagttgcg tggacaatat cacccggatg caggtggcgc tgatgcaccc tctggatgtc
    13021 catgatatcg ccgtcactct gaatgccgac agccgggcat tgcgcagcca ctggtttgtg
    13081 cgcgagaacg gcaccctgct ggagtccagc cgggggctgt ccgggattga tgaaattaag
    13141 caactgttcg gcgcaaaaac gctgacagtg gataccggcg cagataacgc cgcaggaaag
    13201 ctgaccttta acattgacgg gctggcaagg gcgattgccc ccctgcgcga tgcctgccac
    13261 tgggcgggag aataaaaata tggcaatgga cttacgcgat ccgaatgtct ggatatcgca
    13321 cctgctggaa aacctgccgg aagaaaaact ggcatcggcg ctgaaagatg acaacccgga
    13381 ctgggagtat atcgacggcg aaatcgtcaa gctggggtct ctggcccacg ctcagcttga
    13441 tattcccgaa ctacaacgca gggggctaca gcttctggct tctgaaagca aagacttcag
    13501 gctactggca cacctgctca gaaccctgca acatgccggt gatccactgc tggcactgca
    13561 cctgctaacg ctatacgtgg aacattactg gactgtggcc gcgccgcaga atatggcgca
    13621 taaaaaacgc tttgccagcc agatcattaa acgttttgaa acgggtattg aaggcttttc
    13681 acaaaacgct gccacaacgc agcgcgatac tctgctgggt gagctggcga aactggcgca
    13741 gtgctggcag tcacataacg tcccggaact ggcacaggct accgatgatc tttttgccct
    13801 gtaccagcgt acgtttaatc gtgcggctcc tgctccggtc cccactccgg cggcctccgg
    13861 tagttcacca caaaccaccg tcacgtctga aagcggcgtg acgcaaccca gtgctccggc
    13921 tccccaaatc gccatcgaca gtcacgacga caaagcctgg cgcgacacgc tgttaaaagt
    13981 ggcggctatt ttatgtgaac gccagccgga ctcgccgcag ggctatcgcc tgcgccgcca
    14041 tgccctgtgg caatccatca ccagtacacc ccaggcggaa agcgatggac gtaccccact
    14101 ggctgcggtc tctgccgata tggtggcgga ttaccagtcc cggcttgcca gcgcggatat
    14161 ggcgctgtgg caacaggttg agaaaagcgt attgctggct ccttactggc tggacggtca
    14221 ctgtctttct gcacagacgg cactgcgtct gggttacaaa caggtggcag acaccatccg
    14281 cgatgaggtc atccgcttcc ttgagcgtct gccccagctt accgggctgc tgtttaatga
    14341 ccgcacaccg tttctcagtg agcagacgaa acaatggctg gctgcttcgc ccgacggcaa
    14401 agttgcaccg gttgcgcaaa tcggtgagga atcgcaggca gccagagcct gttttgctgg
    14461 gcagggtctg gaggcggcgc tgcgatatct ggacatgcta cccgaaggcg atccccgcga
    14521 tcagtttcac cgccagtacc ttgccgcaca gttgacggag gaggcggggc tgatacagct
    14581 tgcgcagcaa cagtaccgga tgttgttgat gatagggagt cagatgatgg tgtctgactg
    14641 ggagccatca ttacttacgc agcttgaaca aaaattcacg gcagaacaat aaaagagaac
    14701 aggagttttt gtgttcagat taccaacacc ccgactactc agcggactca aatcagccct
    14761 gcgaccggcg atgccccggt ttaaagtctc cgctttctgg ctgctgatac tggcgtggat
    14821 ttttctgctt gctgatactg gcgtggattt ttctgctggt atggatctgg tggaaaggcc
    14881 cgacgtggac gctgtatgaa gaacagtggc tcaaaccact ggcgaaccgc tggctggcaa
    14941 cggcggcgtg ggggattatt gccctggtgt ggcttaccgt ccgggtaatg aagcgcctgc
    15001 aactgcttga aaaacagcaa aaacagcagc gcgaagaggc cattgatccg ctcagtgtgg
    15061 agctgaacgc ccagcagcgt taccttgacc gctggctgct gcgcctgcaa cgccatctcg
    15121 acaaccgccg tttcctgtgg cagttaccgt ggtatatggt catcggcccg gcgggcagtg
    15181 gcaaaacgac actgctgcgc gaggggtttc cgtcagacat tatttatgcc ccggagggcg
    15241 cgcgcggcac agaacaacgc ctgtacctta cgccccatgt cggtaaacag gcggtgatct
    15301 ttgatatcga cggcacactc tgcgctcccg ctgatgcgga tatcctgcat cgccgtctgt
    15361 gggaacatgc cctcggctgg ctgaaagaaa agcgcgcgcg ccagccgctg aacgggatta
    15421 ttctgacact cgatttaccc gatctgctga ccgcagacaa acgccaccgc gagcatctgt
    15481 tacagacgct gcgcagccgc ttacaggata tacgccagca tcttcactgc cagttaccgg
    15541 tttacgtggt actgacccgg cttgatttat tacagggttt tgccgccctg ttccagtccc
    15601 tgaacagaca ggatcgcgat gcgattctgg gcgtcacgtt cacccgccgt gcccatgaaa
    15661 atgatgactg gcgaacagag ttgaatgctt tctggcagac atgggtggat cgaatgaatc
    15721 tggcgttgcc ggatctgatg gtcacccaga ctcacacccg cgcgtcttta ttcagttttt
    15781 cccgccagat gcagggaagc cgtgaaccgc tggtgtcact gcttgagggt ctgcttgacg
    15841 gtgaaaatat gaacgtgatg ctgcgtggtg tctatctcac ctcttcgctt cagcgcgggc
    15901 agatggatga tatattcacc cagtctgccg cccgccagta ccgactgggc aataacccac
    15961 tggcgtcctg gcctctggtg gacaccgcgc cttatttcac ccgcagcttg ttcccgcagg
    16021 cattactcgc agagcctaat ctggcaacgg agagccgcgc ctggctgatg cgttcccgtc
    16081 gccgcctgac ggttttctct gccaccggcg gcgtggcggc actgctgctc atcaccggct
    16141 ggcatcacta ttacaacggt aactatcagt ccggcatcac cgtgcttaag caggccaaag
    16201 cctttatgga tgtgccgcct ccacaggggg aagatgacta cggcaacctg caactgccgc
    16261 tgctgaaccc ggtacgcgat gccacactgg cctatggcga ctggggcgac cgcagccgtc
    16321 tggccgatat gggactgtac cagggacgac gtatcggacc ttatgtggaa cagacctatc
    16381 tgcaactgct ggagcaacgt tacctgccct cgctgtttaa cgggctggtt aaagcgatga
    16441 atgccgcgcc gccggagagt gaagaaaaac tcgcggtgct gcgcgtgatg cgaatgctgg
    16501 aggacaaaag cggacgtaac aatgaggtgg tgaagcagta tatggcaaaa cgctggagcg
    16561 aaaagttcca cggccagcgc gatatccagg cacaactgat gtcccatctt gactacgcgc
    16621 tggctcatac tgactggcac gcagagcgtc aggcgggcga cggtgacgcc atcagccgct
    16681 ggacgccata tgacaagccc gtggtatcag cacagaaaga actgagcaaa ctgcctgtct
    16741 accagcgggt ttaccagagc ctgaaaacgc gggcgctggg cgttcttcct gccgacctca
    16801 atctgcgtga ccaggtaggg ccaacctttg accaggtgtt tacatctgcc gatgacaaca
    16861 aactggttgt tccacagttt cttacccgtt acggcctgca aagctatttt gtaaaacagc
    16921 gcgatgaact ggttgaactg acggcgatgg attcctgggt acttaacctt acccgcagcg
    16981 tgaaatacag tgacgccgac cgcgcggaaa tccagcgcca gctcaccgag cagtatatca
    17041 gcgactacac cgccacctgg cgggccggga tggacaatct gaatatccgc aattttgagt
    17101 ccatcggaca actgaccggg gcgctggagc aggttatcag cggcgaccag cctttgcagc
    17161 gggcgctgac cgtgctgcgt gacaacacac agccaggcgt cttttctgaa aaactctctg
    17221 ccaaagaacg ggatgaggcc ctggcagagc cggactacca gttactcacc cgtctcgggc
    17281 atgagttcgc cccggaaaac attaccctgg cagtacagaa agacaaagaa agcacgatgc
    17341 aggccgtgta tcagcaactc accgagttgc accgctacct gctggcaatc cagaacgcgc
    17401 ctgtaccagg gaaatcggcg ctgaaagccg tgcagttacg gcttgatcag aacagcagcg
    17461 atccgatatt cgccacccgc cagatggcaa aaacgctgcc tgctccgctc aaccgctggg
    17521 ttggcagact gactgaccag gcctggcatg tggtgatggt ggaggctgta cattatatgg
    17581 aagtggactg gcgcgacagc gtggtgaaac cgtttaacga gcaactggca aataactatc
    17641 cgtttaatcc gcgttctgca caggatgcct cactggatgc cttcgaacgc ttctttaaac
    17701 cggatggcat actggatacc ttctaccagc agaacctgaa gctgtttatc gataatgacc
    17761 tgagtctgga ggatggcgat aacaacgtca ttattcgcga agatattatt gcgcaactgg
    17821 aaactgcgca gaaaatccgt gacatcttct tcagcaaaca gaacggtctg ggaacatcct
    17881 ttgccgtgga aacggtatcg ctttcaggca ataaacgccg cagtgtactg aaccttgacg
    17941 gtcagttagt cgattacagc cagggccgta actataccgc ccatctggtc tggcctaaca
    18001 acatgcgcga aggcaacgaa agtaagctga cgctcatcgg caccagcggc aacgcgccgc
    18061 gcagtatcag cttcagcggg ccgtgggcgc agttccgcct gttcggggcc ggacaactga
    18121 ccggagtaca ggatggcaac tttaccgtgc gctttagcgt ggacggtggc gcgatgacct
    18181 accgtgtgca taccgacacg gaagataacc cgttcagcgg tgggttgttc agccagtttg
    18241 gtctgtcaga cacactgtac tgaggaggca gggatgaata gtaacgtact gacacaaact
    18301 atcgttaccg gcagtgaccc gcgcgggctg ccggaattca gcgccatccg cgaggaaata
    18361 aacaaagcca gccacccgtc acagcctgag ctgaactgga aactggtgga gtcgctggcg
    18421 ctggcgattt ttaaagccaa cggtgtggat ttacacaccg ccacctacta tacgcttgcc
    18481 cggacacgga cacagggact ggcgggattc tgcgaaggtg cggaactgct ggcggcaatg
    18541 gtaagccacg actgggataa gttctggccg cagggcggcc cggcgcgtac tgaaatgctg
    18601 gactggttta actcccgaac cggcaatatt ctgcgtcagc aaatctcctt tgcggaatcc
    18661 gacctaccac tgatctaccg cacagagcgg gcattgcagc ttatctgcga taagctccag
    18721 caggtggaac tgaagcgcgt tccgcgcgtg gagaatctgc tctattttat gcagaacacg
    18781 cgtaaacggc ttgaacccca gctgaagagt aacactgaga acgccgcaca gaccacggtc
    18841 agaacgctga tttatgcccc ggaaacacag gcatcttcca caccagaagc ggtagtgcct
    18901 cccctgcccg gcctgcctga gatgaaagtg gaagtgcgca gtctgacaga gaatccccca
    18961 caggccagtg tgataaagca aggcagtacg gtaagagggt ttatcgcagg gatcgcctgt
    19021 tcagtggctg tcgcctcagc attgtggtgg tggcaggtct atccggtgca gcagcaactg
    19081 ttacaggtta acgacaccgc tcagggcgca gcaacggtgt ggatggcctc acctgaactc
    19141 gaaaactatg agcgcaggct gcaacaactt cttgatacct ccccggtaca gccgctggaa
    19201 accgggatgc agatgatgcg tgttgccgac agtcgctggc cggaaagcct gcaacagcaa
    19261 caggcctcga cacaatggaa tgaggcactc aaaacccgcg cacagagtag cccgcagttg
    19321 cgtggctggt tgcagacccg ccaggactta catgcttttg cagatctagt gatgcagcgc
    19381 gagaaagagg gactaaccct ttcctatatc aaaaatgtca tctggcaggc ggagcgggga
    19441 ctggggcagg aaacacccgt tgagtctctg ttgacgcagt accaggatgc ccgtgcgcag
    19501 aagcagaata cagatgcgct ggaaaaacaa attaatgagc gactcgaagg cgtgttaagc
    19561 cgctggctgc tgctgaagaa taatacgata ccgacgataa aaaaagcatt gaatttcaat
    19621 aacattcatg aatacaa
//
LOCUS       DBFIFI010000072        19512 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.072, whole genome shotgun sequence.
ACCESSION   DBFIFI010000072 DBFIFI010000000
VERSION     DBFIFI010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19512
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.072"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            143..331
                     /locus_tag="N7B39_002467"
     CDS             143..331
                     /locus_tag="N7B39_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2422432.1"
                     /translation="MPENRRIILDSKESWLIICDSQLGHLMRSMYQGRRFIQLNLEKL
                     KGVHDVALPVKWEFTRRQ"
     gene            626..2251
                     /locus_tag="N7B39_002468"
     CDS             626..2251
                     /locus_tag="N7B39_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2422433.1"
                     /translation="MPYIDITTMRGMMPRVVTSMLPEHSAVLAEDCHFRFGVITPERQ
                     ISGLEKTFTIKPKTIFHYRDDFWFAWPDVVDVIRSPIAQDPHGRIYYTDGRFPKVTDA
                     TIATKGDGNHPTSSYRLGIPAPTTAPVCTVQQGGDVSDDNPNDDETRFYTETFVSDYG
                     EEGPPGPASLEVTLRTPGTAVQLTLAPVPLQNASIKRRRIYRSASGGGEADFLLVAEL
                     DASVLSYTDKIPAKNLGPSLATWDYLPPPENMTGLCLMANGIAAGFAGNEVMFSEAYL
                     PYAWPEVNRHTTAEDIVAICPLGTSLVVATKGEPYLFSGVSPSTISGSKIPSMQACLS
                     RRSMVAMEGFVLYAGTNGLVSVDANGNVALATEQIVSPEQWQSQFNPASIVAYSWRGE
                     YIACYTKPDGKQDVFVFSPVNMDIRYLSTPFDCAWVDLAKDMMRVVTGDKMSVLAGGS
                     LPSTIRWHSKIFSLPERTSFSCIRVKSPAPERVGITIMADDVPVIHFAPGTFKGSVVR
                     LPAATGQNWQVMVSGFGQVERITLSTSMSEMPV"
     gene            2248..3516
                     /locus_tag="N7B39_002469"
     CDS             2248..3516
                     /locus_tag="N7B39_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HGU2422434.1"
                     /translation="MTRKPWRAGKDLSTVVENMEIGTGQRGDGRHAFVTREELVGLKL
                     ARRRTSGGASYALNPGIEIDSTLMTVDFPTKPLNFKAAGGFGSVLLEWDMPNYRGHSL
                     TEIWRGTEDDLADAVLVATTPGQVYGDPVDPGWSGFYWIRFVNAAGVKGPWNAEKGTQ
                     AQTQIGVKAIIDQIRDEAAKSPVVSELRKEIKNAQGQAVKDAAIKTTEVVGTLREETT
                     RTIGGIETRISTLDSSTSESLNEVDKRITKLDKEGGEAFLAMWSKKAGVDGITAGIGI
                     VAGKDSEGRPVSQVAISASQLFVFDPNNPDNTAYPFAVSGGKVVIPKAMIYDAVIETL
                     VSRKVVADEVKAGVSITSPVIRSAVIQNGNFQVDSQGNLNIGGLFSVTSQGQLTIRYS
                     NQNVGLVIRNDKIEVYDQNGRLAVRIGRLR"
     gene            3531..3809
                     /locus_tag="N7B39_002470"
     CDS             3531..3809
                     /locus_tag="N7B39_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein"
                     /protein_id="HGU2422435.1"
                     /translation="MEYGFAIYNRNNVNVTGVLTPIFFLDRFTAESGSKTYTNKPEGK
                     TLQAVCSLFPWNNVFKDRKVPKITINGNTVTWSNLEQGMGSYIYTFWG"
     gene            3815..4432
                     /locus_tag="N7B39_002471"
     CDS             3815..4432
                     /locus_tag="N7B39_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2422436.1"
                     /translation="MYGLSITKPDGSLWISPGFTPQCLINKGTIPATEKAFFKTSIPS
                     GKSCFFFIRTENKADVMYTHEQIDGYHALRLHQIVRGTNPGVTTVYAFANMVTQPSEY
                     GIAMYNPSGEMIYHGEMMLLDAKLIPVDIKFEKDLGYPCAIMPALVGYYNWKRTPYDR
                     PIYTTSTGATGNKIYSCEHYSGGATWDIRKPYIDKVLVINSSIYD"
     gene            4512..5249
                     /locus_tag="N7B39_002472"
     CDS             4512..5249
                     /locus_tag="N7B39_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HGU2422437.1"
                     /translation="MKSIMKFFACAVLVMSCLTAQVNAASGDSTVSLGFAHIRFPGLK
                     DFVRDAGVYNRDTFRNVVNVNHFNSSGEYENAIARGHDGTAKSPLGMSIRYRYEITDE
                     LGVIASFTWARSMTNAQAFIDVKPSDPSREVKNPAASARTDIRANYWSLLSGPSWRFN
                     EYLSVYAMAGMGVAKVTTDLKINDNLNHGAGSFSESNSTKKTSIAWSVGAQFNFNESV
                     TMDVTYESSGSGDWKTDGLFAGIGLKF"
     gene            5482..5622
                     /locus_tag="N7B39_002473"
     CDS             5482..5622
                     /locus_tag="N7B39_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HGU2422438.1"
                     /translation="MSQKSLITVTICMTVIFTIWMLHGSLCEFRLNLWGAEFAAFLQC
                     KQ"
     gene            5679..6080
                     /locus_tag="N7B39_002474"
     CDS             5679..6080
                     /locus_tag="N7B39_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2422439.1"
                     /translation="MAPVSGERMDDKILRYMQRVVRNSRNPEFMNEVKDACLKKQAFC
                     FEAPDGFLVLRSVLSDDGIPYVLVLLGVCTGSAGVERYLPEVKTLTSLAGGRWAEFHT
                     ARRGFIRLGKRLGFERMPDDEDGFMVFRIAV"
     gene            6174..6827
                     /locus_tag="N7B39_002475"
     CDS             6174..6827
                     /locus_tag="N7B39_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2422440.1"
                     /translation="MGGKKGGGDTKVKPTAAQIAQEEVAWKGWQDYKNILRPAEDNFM
                     EKVDDLNSEQQYDNIAGTTNLGYQKQFGETRRELAGNLAQSGVDPSSGRFNAVMDANQ
                     SDQVTGQIDTTTRGQVSQADKYIAGLQDVAALGSGQKADALQSFNSLADSSLAKAKSD
                     AQAAFTKQQGRASLVGAGLGAVGAYAMHKAGGSGSGGSKTPDTGANAIQSQAQNWRL"
     gene            6830..7276
                     /locus_tag="N7B39_002476"
     CDS             6830..7276
                     /locus_tag="N7B39_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2422441.1"
                     /translation="MEYGKYETLARAGYSGADRPQGDWQTSAALTRQQYDDWRTRYLP
                     RVARLADLGENNSLMNAQLARVGGLATSSLRTAQMAQDNQMARYGVNRPDNPNSNTLG
                     LRNALAIAGAKNGIREAEQDRQMNILTGASAPARQQLSVGGQMMTA"
     gene            7287..7538
                     /locus_tag="N7B39_002477"
     CDS             7287..7538
                     /locus_tag="N7B39_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HGU2422442.1"
                     /translation="MGYGLLDIANQSRREALQGISDADRRREEIEAANKQMAAQQKAQ
                     NKQNIGTGIGTGAAIGASVGGPVGAVAGAVIGGIAGSLF"
     gene            7549..8814
                     /locus_tag="N7B39_002478"
     CDS             7549..8814
                     /locus_tag="N7B39_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2422443.1"
                     /translation="MSGFAQGLLAGFSTVDQAMTRRKELGLREAQLAQQQKNNERDFE
                     FAQSQFEHNKDVDQRNFDYRAKVDDRNYALQEREFNANQNYRNASLGMEQQRLQLQKY
                     NQRRLEYNDMLARDQSVMVALGKAIDAGDHDAASRLFGKLSDANPIKIISTEGYAAKA
                     GQAVNNLQKIFDDKPDRAIDSLNTPENLDVLSGVFAPELQQRIGMPDSTGEKTIKEAR
                     IGSIVPAQQEGYVLIGLDLTYSDGSTAHKPVTEYGSAHPDDQTVLAIPVDKAIAQVRD
                     RSKFAEISKNYGYFMPKQQGLSLKELQKGASNVAADAIKNGGNAQAAVDEYYAATGSQ
                     PHQQKIQQQKLQQQVINWAGDDPDKLSFARNVAARQPEMLEPQNQKLLENGYANFLRI
                     QKARGEQARDESASSASQFIRGLKQNYAQ"
     gene            8884..17238
                     /locus_tag="N7B39_002479"
     CDS             8884..17238
                     /locus_tag="N7B39_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD38 domain-containing protein"
                     /protein_id="HGU2422444.1"
                     /translation="MAYSEEQRPEAQLGNQNRNSLNIQQPGETDSYEAFFSDSNRWKD
                     NSTSFSLGDVLPTMGKGFARSVRGTGEMARGLGDAMIQSPVKTGARILNEFSRMGLPG
                     VATVQDIFAGGSRGADEVIDTLPDGKNAVTDTVGKGLKVTGKAVSDGAKSSDEWLTSK
                     MSPGALRALNTPMTEGYDDSAVWVAKGVNLIGALVPDMVAGGVAKKVGDVTLRKTLTA
                     GLEKKYIAAGMQPERATALAAEAVDKKMPDLFQAGLITHSTASAQGQSAMAAADAVLN
                     ADYSELAQSPKFQQTFLSIDADPQHAQLTDRQKMDLAKERVADEVRAQLATDPELLAV
                     NAMAAKLGDAQLLNLAMRGTANTVKSGIVRNATEQGTINAAQGGYSRYQENTALRETA
                     GMDVSPWEGVADATIEGAALGAAMGAPFGAAAGYRGKRQAADEAAMREAESVPQDESA
                     PQPEPVDPVAQHRESMQGMNREQLLEQYADADMAHEGDTSAVHRREAASQLLNELDEQ
                     AKRQAVMDELKAKPRHELLEEYRKLSQKEGRTDTEEQQLQAIRDVLRPQREARPEAQP
                     QPENADDGDGSIYPTVRFRDPDEVRIEINESGASRPAERIEKVRPDNRYFTDEKSALG
                     SDVFRNASATGLKPSVVKKGENQYAVEMDNPAFSEDVATETINTLADGERITDADPME
                     QPAFMRDPRFRGFTGDDTEVQARLARGNAPTAEELVRSQMAEGDAGPTAQELTERPRL
                     PAPGDIHPGQGYPLPGEVARTPDENQAGRGGRFTTTGEVKGQSFQKGRAQAPENAAGR
                     QGEILEGETVRRGLPSPDEQNATAPVREGLPAPESQRGVDMPQPESLPRMVRDSLPEL
                     AQQAEARRQTGDNRQTITDVPDTEVPVPVDKPSTHQQARGAKIEDFGEEIKGAAKHRY
                     AQLAEAMGKTLEDGEYATQPLSKLFPKPDYARLEKEGVDSDTLAMMALYRSEIPTRTS
                     RNMQKWISIVKSGREATAGMLEGKIPAAKLADMMDSKPGLRSMSDTWKLLRTLSALQI
                     DKASGYRVRSGVYSFVGGKRYDPPQMMYSLRDSKGRDLFFSESRDELLKKAKAYFDEQ
                     GSRERETPATSADDRITFDVYRHKASGDIFIGYGKNRQKLKGGFESARDAHDYVRTHR
                     DELVNQVKALREVSREEQRNATNRDRTGPERRKGDVSPEQFSDAFGFRGVQFGNYVES
                     PRRQADLNRAYDSLHDLADVLNVPTKALSLNGRLGLAFGARGKGKAAAHYEPGEVAIN
                     LTKGNGPGALAHEWFHSLDNYFGRYDVSTDGKITSGGDYMTEAQRAGRVFKDGRYVDA
                     EYPVRQEVYDAFKGVMKAINSSDMLRRSERLDGVRSKPYWSTDVEMAARAFERYVQDK
                     ARMAGVENDYLVNIRKADDHGQPDTYAYPTNAELDGGIREAFDHLFRTLKTRETDKGV
                     AFYSRKGVTRTPEGNLISDVNRSAEAKGSPVPQVEAVARGVMSGIKDSDLKVRVVKSQ
                     KEAEALAGELFDGYGRVHAFYRPDKREIVLVADNIPDGRTVREKLRHEIIHHAMEHVV
                     TPAEYQTIIKTVLKTRDSDNVTIREAWRKVDASYGKESPEVQAGEFLAHMAEKQPNKF
                     VAAWERVVALVKGVLRRTGLLKPTELNDIRLVRETIRTLGLRVREGYTPREDGAGASS
                     QYSRSGKPDPFKVPEGEGERYRDDLARMMKSLRSGAMTANIGRTPPVLRHLGAPDLPL
                     VISRDTVRKATNGVKHVVPMDVIERLPELMHDPDAIYRSATERNAVVMLLDAVDKNGD
                     PVVSAVHMKATDKRIEINKVASVYGTKGGMNKANSLDKAGLTLYRKEKLSRDNPQYSG
                     LQLPKEERSYRGSVDKILYPEDIRNGPYYSRTSSLTPEETIASRFVRQMQDKFQVLKA
                     VQENIRKTGGKIDDSNNAYMAEELFHGKAENDLNVMKERYVQPLAKLLADYKIAQADL
                     DEYLYARHAPERNAHIAKINPKMPDGGSGMTNAEAAEIMQRVRNSGKQAQYDRLAGII
                     DDMLARRRELIRQAGLEENGVVDAWQNAYRYYVPLKGQDVDGVVSLPRTGKGFTIGGR
                     ESRQAMGRASRAQSPSTQAIQDLSESLIRHRKNEVGNAFLKLVQDNPDKDYWQVFTDD
                     RPDTMRTIAERKDQETGETIREVVERPVPMAMMADRYFTTKKNGKTYYIKLHDPRLMR
                     AMKNMGPETSNAVIRTLGKVNRFLATVNTSYNPEFLVSNFIRDVQTAVMNLKAEQGRS
                     DGKLKGLDNLSALAVVKDSRSAMSAVYASLRGKTLTGNGAQWQKVWKEFVEDGGKTGW
                     FNMGDLEGQQKEMDRLVSLAKGGWKGQSIGAWNSFLNLVEDANGAVENALRLSAYKHA
                     RDAGLSRQQAASLAKNMTVNFNRRGEQGALMNSLYMFANASIQGTANLVRTLGHLNGD
                     GPLLERLRWKNLNVPQKIALAAVGAGYLLGSLNRSVAGEDDDGVNWYDKVPSHVKERN
                     LVIMKSVFGGKAGEYWSIPLPYGYNVFFLLGHTAEGVAAGDLTASRAAGNVVGGVLGA
                     FSPVGSETSETLSGALLKNAAPTILRPFANLAMNENFMGAQIYQENIPFGAPKPDSQL
                     GRRSTPEAYKAFASWLNAFSGGSQYRPGAVDITPESLKFWIDYISGGTGRFISKTTDA
                     AVKSLNGIDIPEQQVPFLGKISGEVMPYADQQKMYDRMTEVAQYHAELKSLTGAERTA
                     FIDENNGKLSMNGLMQDTRKRLKDLRKQRDAIYADSTLSLAQQSAMVKSVERDMKIAV
                     DRFNREYNKKVGVE"
     gene            complement(17460..18392)
                     /gene="yfdC"
                     /locus_tag="N7B39_002480"
     CDS             complement(17460..18392)
                     /gene="yfdC"
                     /locus_tag="N7B39_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HGU2422445.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            18686..19441
                     /gene="mlaA"
                     /locus_tag="N7B39_002481"
     CDS             18686..19441
                     /gene="mlaA"
                     /locus_tag="N7B39_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HGU2422446.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT         4992 a         4367 c         5631 g         4522 t
ORIGIN      
        1 tagccgacag cttcacaaaa ccggagcccg gctccggttt ttgttgtcat gtataggggg
       61 ggggttatta gagagtgaag taataaacat gttaatacga tggagtgaag gatgccgtgt
      121 aattctggtt caagagtttt ttatgccgga aaatcgcagg attattctgg atagtaaaga
      181 atcctggtta ataatctgtg atagtcagtt gggccattta atgcgcagta tgtatcaggg
      241 acgccgtttt attcagctga atctggaaaa attgaaaggg gtacatgatg tcgccttgcc
      301 agtgaaatgg gaattcacac gaagacagtg aatagctttc tgtatacggg aatggcgaaa
      361 aatggactgt atggtgtgag tgtgaaacat cttgcgtgtg cggagtgatg cttctcgttg
      421 ctacagcggc aatgataatg cagtgaaaaa aggggagcaa tatgctcccc caaaccgaaa
      481 gaaaattaca ataatcaatg aagttattta gtcatcatca gaatgtcatg caaggcattt
      541 tgtttcagtg atgccgatcg cgattttagc gaattccatc ataaatcccc tgatttttaa
      601 gcctgaagca gtcaaaggaa tttctatgcc ctatatcgat atcaccacga tgcgtgggat
      661 gatgccgcgc gttgtgacat ccatgctgcc cgagcattcc gctgtactgg cggaggactg
      721 ccatttccgg tttggtgtta ttacaccaga acgtcagata tccgggttag agaaaacatt
      781 cacaattaag ccaaaaacaa tttttcatta ccgtgacgat ttctggtttg catggccgga
      841 tgtggtggat gtgatccgca gtccgatcgc tcaggacccc cacgggcgta tttactacac
      901 tgacgggcgt tttcctaaag tgacggatgc gactattgcc acaaaagggg acgggaatca
      961 cccgacatca tcgtatcgtc tggggatccc cgcgccgacg acagctcctg tctgtactgt
     1021 tcagcagggc ggtgatgttt ctgacgataa cccgaatgat gatgaaaccc ggttttatac
     1081 ggaaaccttt gtctcagatt atggtgaaga aggtccgcca ggtccggcgt ctctggaggt
     1141 aacactccgt actccgggaa ctgcggtaca actgacgctg gctccggtgc cattgcagaa
     1201 tgccagtatt aaacgtcgcc ggatttatcg ctctgcatca ggtggagggg aggcggattt
     1261 tttacttgtg gctgaactgg atgcatccgt gctcagttac acggacaaaa taccggcgaa
     1321 aaaccttggg ccttccctgg cgacatggga ttacctgccg ccgccagaga atatgacagg
     1381 cctttgcctg atggctaacg gtattgccgc cgggtttgcc ggtaatgaag tgatgttttc
     1441 ggaagcgtat ctgccgtatg catggccgga agtgaatcgt cacacgacgg cagaagatat
     1501 tgtagctatc tgtccgctgg gaacgtcact ggtggtggcg acaaaggggg agccttattt
     1561 gttcagtggg gtatcaccgt ccacaatttc tggttccaaa atcccttcaa tgcaggcgtg
     1621 tctgagcagg cggagtatgg ttgcgatgga gggctttgtg ctgtatgcag gaacaaatgg
     1681 cctggtgtct gttgatgcaa acggtaatgt cgcgctggcg acggaacaga ttgtttcacc
     1741 ggaacagtgg cagagtcagt ttaatccggc ctccattgtg gcttattcct ggcgtggtga
     1801 gtacattgcc tgttacacga aaccggatgg taagcaggat gtgtttgtat tcagtccggt
     1861 gaacatggat atccgttatc tcagtacacc gtttgactgc gcatgggttg atctcgcgaa
     1921 agatatgatg cgcgtggtga caggagacaa aatgtcagtg cttgccgggg gctctctgcc
     1981 ctccacgata aggtggcatt caaaaatttt ttcattacct gaaagaacct ctttttcctg
     2041 tatcagggtg aaatctccgg cgcctgagcg ggtggggatc accattatgg ctgatgatgt
     2101 tcctgtgatt cattttgcgc cgggtacgtt taagggaagt gtggtgagac ttccggcagc
     2161 aaccgggcaa aactggcagg tgatggtatc cggattcggg caggtggaac gaataaccct
     2221 gagtacatcg atgtcggaga tgccggtatg accagaaaac cgtggcgtgc ggggaaggat
     2281 ttatccacag ttgtggagaa catggaaatt ggcaccgggc agcgtggtga cggacgccac
     2341 gcatttgtga cccgtgagga actggttggt cttaaactcg cccggcgtcg aacatcgggt
     2401 ggtgcctcat atgcactgaa tccgggtatt gagattgaca gtactttaat gactgttgat
     2461 tttcccacaa aaccgctgaa ttttaaggcg gccggtggat ttggctcggt tcttcttgaa
     2521 tgggatatgc ctaattatcg cggacattca ctgactgaaa tctggcgggg tacggaggat
     2581 gaccttgctg atgcagtgct tgttgccacg acgccggggc aggtttacgg cgatccggtt
     2641 gaccctggct ggtcgggatt ttactggata cgttttgtta acgcggcagg agtgaaaggt
     2701 ccatggaatg ctgaaaaagg cactcaggca caaacacaga tcggcgtgaa ggccatcatt
     2761 gaccagatcc gcgatgaggc tgcaaagtcg ccggttgtgt ccgagctgcg taaagaaata
     2821 aaaaacgcgc aggggcaggc tgtaaaggat gctgcaatta agacaaccga agttgtgggg
     2881 actctcaggg aagaaacgac aagaacgatt ggtggtattg aaacccgcat tagcacactg
     2941 gattcgtcaa ccagtgaatc gcttaatgag gtcgacaagc gcatcactaa actggataaa
     3001 gaaggcggtg aggcgtttct ggcaatgtgg tcaaaaaaag cgggagttga tggtatcact
     3061 gcggggatcg ggattgtcgc cggaaaagac agtgaaggca ggcctgtaag tcaggttgca
     3121 atttctgcgt cgcagttgtt tgtctttgac ccgaacaacc cggataacac cgcctatccg
     3181 tttgcggtat caggtggcaa ggttgtgatc ccgaaagcga tgatttatga cgcggtgatt
     3241 gaaacactgg tgtcgcggaa ggttgtggcg gatgaggtaa aagccggggt aagtatcact
     3301 tcgccagtta tccggagtgc cgttattcag aacggaaact ttcaggttga ttctcagggt
     3361 aacctgaata ttggaggcct tttcagtgtt acgtcacaag ggcaactgac aattcgttac
     3421 tctaatcaga atgtaggact ggtgatccgc aatgataaaa ttgaggttta tgatcagaat
     3481 ggacgactgg ctgttcgcat aggcagatta cgctgatcag gaggtgagta ttggaatacg
     3541 gttttgccat ttataacaga aataacgtta atgttacggg cgtgcttact cccatatttt
     3601 tcctggacag gtttacggcg gagtcgggat caaaaacgta tacgaataaa ccagaaggga
     3661 aaacattgca ggcagtctgt tcattatttc cgtggaataa tgtatttaag gaccggaaag
     3721 taccgaagat aaccattaat ggcaatacgg tgacgtggtc aaatcttgag caggggatgg
     3781 ggtcatatat ttatacattc tgggggtgag ttgtatgtat ggtttaagca ttacgaaacc
     3841 agatggcagc ttgtggataa gcccggggtt tacgcctcag tgtctgatca ataagggaac
     3901 tattccggcg actgagaagg ctttttttaa aacgtcaatt ccgtcaggaa aaagttgttt
     3961 tttctttatc agaacagaga ataaggccga tgtcatgtac acgcatgaac agattgatgg
     4021 atatcatgcc ttaaggcttc atcaaattgt caggggaacg aatcctggtg ttacgactgt
     4081 ttatgctttt gcgaatatgg ttactcaacc ttctgagtat ggtatcgcca tgtataaccc
     4141 gtcgggtgag atgatttacc atggcgaaat gatgctgctt gacgcgaagt taataccagt
     4201 tgatatcaag tttgagaagg accttggata tccatgcgca attatgcctg cactggttgg
     4261 atattataac tggaaacgaa caccttatga ccgaccgatt tacaccacat ccactggtgc
     4321 tacaggaaat aaaatatatt cctgtgagca ttattccggt ggtgcaacat gggatatccg
     4381 aaagccgtat atagataaag ttctggttat taattcctca atatatgatt agcgtaagaa
     4441 aaactttaat agtgtaaaac gaaagcgttc ctttcagaca tcgcatcctt taattcagag
     4501 aaatacttaa aatgaaaagt atcatgaaat tttttgcgtg tgcagttctg gtgatgagtt
     4561 gcttaactgc acaggtaaat gcagcatcag gcgatagtac ggtatctctc gggtttgcac
     4621 acatacgctt ccccgggctg aaggattttg ttagagacgc gggtgtttat aaccgggata
     4681 cgttcaggaa tgttgtaaac gtaaaccatt ttaattcatc aggtgaatac gaaaatgcta
     4741 ttgcccgggg gcatgacggc acagccaaaa gccccctggg gatgagcatc aggtatcgtt
     4801 acgagataac tgatgaactg ggtgttatag cctcttttac atgggcgcgc tccatgacta
     4861 atgcgcaggc atttattgat gttaagcctt ccgatccatc acgagaggta aaaaacccgg
     4921 cagcttcagc gagaactgat atcagggcta attactggag tctactatcc ggtccttcgt
     4981 ggcggtttaa cgaatacctc agtgtgtatg caatggctgg tatgggggtt gcaaaagtca
     5041 ccactgactt gaaaattaac gacaatctta atcatggtgc cggtagtttt tctgaaagta
     5101 acagcaccaa aaaaacgtct attgcatggt ctgtcggcgc gcaatttaac ttcaatgaaa
     5161 gtgttactat ggacgtcact tatgaaagct caggctctgg cgactggaaa actgatggtt
     5221 tatttgcagg cattggcctg aaattctgag taatacccga caacatgtca taacccgctg
     5281 tgatggcggg ttttttgttg cccgtgcagg gcaaaaatcg tagattatgc gcgggtgcct
     5341 ttcggctgat ggctggaggg agaacctgaa ggcctgatgt ggaaaggccc cgagtcaaac
     5401 attttgttta acccgaggcc ctaaccatca taccttaagc aagtagaagg ttagcgcctc
     5461 tccactcagg aggcaagcgc tatgtcgcaa aaatcgctca tcaccgtcac aatttgtatg
     5521 acggtaatct tcaccatctg gatgttacac ggctcgttgt gtgagttccg gctgaatttg
     5581 tggggagcgg agtttgcggc gttcttacag tgtaagcagt aggaaaaccg cgacggggac
     5641 gaaagtcccc gtcaactggt tgctgaggtt cagccgatat ggcacccgtt tctggtgaga
     5701 gaatggacga caaaattctc cggtatatgc agcgtgttgt gagaaattcc cgcaaccctg
     5761 aatttatgaa tgaagttaaa gacgcctgcc ttaaaaagca ggcgttttgc tttgaggcac
     5821 cggatggttt tctggtgctg cgttctgtgc tcagtgacga cggtatccct tatgttctgg
     5881 tgttgctggg tgtgtgtacg gggagtgccg gtgttgagcg ttatctgccg gaggtgaaga
     5941 cattaaccag tctggctggc ggacgctggg ctgaattcca cacggcaagg cggggattta
     6001 tccggctggg aaaacgtctg ggctttgagc gaatgccgga tgatgaggat ggcttcatgg
     6061 tgttcaggat agcggtctga ctgccactgt attcatcatc gtgtgtaaac caacattgca
     6121 cttcacattc tgaccctgcc ccggcagggt tttttgttat ctgaggagcc ataatgggcg
     6181 gaaaaaaagg cggtggtgat accaaagtaa aaccaacagc agcgcaaata gcacaggaag
     6241 aggtggcctg gaaagggtgg caggattaca aaaatattct ccgtccggca gaagataact
     6301 tcatggaaaa ggtcgatgac cttaacagtg agcagcagta cgacaatatc gccggtacaa
     6361 caaatctggg atatcagaaa cagtttggtg aaacgcgaag ggagcttgcg ggtaatcttg
     6421 ctcagtccgg cgttgaccca tccagtggtc gttttaatgc ggtaatggat gcaaatcaaa
     6481 gcgaccaggt gaccgggcag attgacacga ccacacgggg gcaggtatcg caggcagata
     6541 aatatattgc cgggctacag gatgttgcgg ctctcggttc cggtcagaag gccgatgcgt
     6601 tacagagttt taactctctg gcagacagca gtctggcaaa agccaaatca gatgcacagg
     6661 cggcgtttac aaaacaacag gggcgggcct ctcttgttgg cgctggtctg ggggcggttg
     6721 gggcttatgc aatgcataag gctggcggta gcggaagtgg aggttctaaa acgccagaca
     6781 ccggcgctaa cgctattcag agtcaggctc agaactggag actgtaatta tggagtatgg
     6841 caaatacgaa actctcgcaa gggctggtta ttcaggagca gaccgcccac agggtgactg
     6901 gcagacgtca gcagcgctga cacgccaaca atacgacgac tggcgaacca gatatttgcc
     6961 ccgcgtggca aggctggctg accttggtga gaacaacagc ctgatgaatg cacagcttgc
     7021 ccgggtgggc ggccttgcca cttccagtct ccgtacagcg cagatggcac aggataacca
     7081 gatggcgaga tacggggtaa atcgtccgga taatcccaac agtaacacac tgggtttgcg
     7141 taatgctctg gcaattgctg gcgcgaaaaa tggtatccgt gaagcagagc aggatcgcca
     7201 gatgaacata ctgacggggg cttctgcacc tgcaagacaa caactgagtg ttggcggtca
     7261 aatgatgaca gcgtaagggg gaagatatgg ggtacggctt actggatatt gcgaatcagt
     7321 cacggcgtga ggcattacag ggaataagtg acgcagaccg acgacgtgaa gaaattgagg
     7381 ctgcgaacaa acagatggcg gcgcaacaga aagcgcagaa caagcagaat atcggtacgg
     7441 gcattggtac gggggcggct attggcgcat ccgttggtgg tcctgttggc gctgttgctg
     7501 gtgcagtaat tggcggcatt gctggttctt tgttttaagg agtggtgaat gagcggattt
     7561 gcacaggggt tacttgccgg attcagcact gttgaccagg caatgacccg tcgtaaggag
     7621 cttggtttgc gagaagcaca gcttgctcag caacagaaaa ataacgagcg cgattttgaa
     7681 tttgcgcagt ctcagtttga acataataaa gacgttgatc agcggaactt tgattacaga
     7741 gccaaagttg atgaccgcaa ttacgcactg caggaaaggg agtttaacgc caaccagaat
     7801 taccggaatg cgtcgctggg gatggaacag caacgactcc agttgcagaa atacaaccag
     7861 cgacggcttg agtataacga tatgctggca cgtgatcagt cggtgatggt ggcgctggga
     7921 aaagcgattg atgcaggtga ccatgatgcg gcctctcgtc tgttcggcaa attatcggat
     7981 gccaacccca ttaaaataat atcaacggaa ggctatgctg cgaaggcggg tcaggccgtg
     8041 aacaacctgc agaaaatctt tgatgataag ccggacaggg cgatcgattc gctgaatacc
     8101 ccggaaaatc tcgatgtgct ttccggcgtg tttgccccgg aactacaaca gcgtattggc
     8161 atgcctgatt caaccgggga aaaaacgata aaagaggcca ggattggcag tatcgtaccg
     8221 gcgcagcagg aagggtacgt acttattggc cttgatctca catacagcga tggctccacc
     8281 gcgcataaac ctgtaacaga atacggcagt gcgcaccctg atgatcaaac cgtgctggcg
     8341 atacccgttg ataaggctat cgctcaggtc agggatcgca gcaaatttgc agagatatcg
     8401 aaaaattatg gttattttat gccgaagcag cagggacttt ctctgaaaga gcttcagaag
     8461 ggggccagca acgtagcggc ggacgcgatt aagaatggcg gtaatgctca ggctgcggtg
     8521 gatgaatatt atgctgcgac tggttcacaa ccgcatcaac agaaaattca gcaacaaaaa
     8581 cttcagcaac aggttatcaa ctgggcggga gatgatcctg ataagctgtc atttgccaga
     8641 aatgtagcgg cccgtcagcc tgaaatgctg gaacctcaga atcagaaatt gctggagaac
     8701 gggtatgcga attttctccg tattcaaaag gccagggggg aacaggccag agatgaaagt
     8761 gcttcatctg catctcagtt tatccgtgga ctgaaacaga attacgccca gtaattcacg
     8821 atattccatt aataccattt cctgatgccc ggccattgtg ccgggttttt ttatggagtc
     8881 tgtatggcct attcagagga acagcgtcct gaggcgcaac tcggtaacca gaatcgtaac
     8941 agcctgaaca ttcagcaacc cggcgaaact gacagctatg aagcattttt ctctgattca
     9001 aatcgctgga aggataacag tacgtcgttc agcctgggcg atgtattgcc aacaatgggt
     9061 aaaggtttcg cccggtccgt ccggggaaca ggggaaatgg cccgtggact cggtgatgcg
     9121 atgattcaga gcccggtaaa aacaggggcg cgtattttaa atgagttcag ccgtatgggg
     9181 ctgccgggtg tcgcaactgt gcaggatatt tttgccggtg gcagcagggg ggctgatgag
     9241 gtcatcgata ccctgcctga tggcaaaaac gcggttacag atactgtcgg taaaggtctg
     9301 aaggtcaccg gtaaggctgt cagtgacggc gctaaatcct cggatgaatg gctgaccagt
     9361 aagatgtctc cgggtgctct gcgtgcgctg aatacgccga tgaccgaagg ctatgatgat
     9421 tctgcggtct gggtggcgaa gggcgtaaac ctgattggtg cccttgtacc tgatatggtt
     9481 gctggcggtg tggctaaaaa ggtgggtgat gtcacattgc gaaaaacgct gaccgccggg
     9541 ctggagaaaa aatacatcgc ggcagggatg cagccggaaa gagccacggc actggcagca
     9601 gaagctgtcg ataaaaaaat gccggattta ttccaggcgg gcctgatcac ccattccaca
     9661 gccagtgcac aggggcagag tgcaatggcg gcagcagatg ctgttcttaa tgcggattac
     9721 tctgagctgg cgcagtcacc gaaatttcag cagacgtttt tgtccattga cgccgatccg
     9781 cagcacgcac agcttactga tcgccagaaa atggatctgg caaaagagcg tgttgccgat
     9841 gaggtgcgcg cgcagctggc aacagatcct gaattgctgg ctgtgaatgc catggcggca
     9901 aaactgggcg atgctcagtt gcttaatctg gcgatgcggg gcacggcgaa taccgttaaa
     9961 agcggcattg tcagaaatgc cacggaacag gggacgatta atgcggcgca gggtggctat
    10021 tcacgctatc aggaaaacac ggcattgcgt gagaccgccg gaatggatgt gtcaccgtgg
    10081 gagggcgtgg ctgacgcaac gatcgaaggt gcagcccttg gtgctgcaat gggggcacca
    10141 ttcggtgcgg ctgccggata tcgtggaaaa cgtcaggcgg cagatgaagc agccatgcgt
    10201 gaggctgaaa gcgttcctca ggacgaatcg gctccgcaac ccgaaccggt tgatccggtg
    10261 gcacagcatc gtgaatctat gcaggggatg aatcgcgagc agcttctgga acagtatgct
    10321 gatgcggata tggcacatga gggagacacg tctgccgttc atcgccggga agccgccagc
    10381 cagctgttga atgaactgga tgaacaggcg aagcgacagg cggtgatgga tgagctgaag
    10441 gcgaagccgc gccatgaact gcttgaggaa taccgaaaac tcagccagaa ggaagggcgt
    10501 actgatactg aagagcagca gttacaggca atacgtgatg tgcttcgccc tcagcgggag
    10561 gccagaccgg aggcacagcc acagccggaa aatgcggatg atggcgacgg gagtatttac
    10621 ccgacggtgc ggttccgcga tccggatgaa gttcgtattg aaattaacga gagtggtgcg
    10681 tccagaccag cagaacgcat tgaaaaggtg cgtccggaca accgttattt cacggatgaa
    10741 aaaagtgctc tggggagcga cgttttccgc aatgccagcg ccaccggcct gaaaccgtcc
    10801 gtagtgaaga aaggcgagaa tcagtatgcc gttgaaatgg ataatcctgc gttctctgaa
    10861 gatgtggcaa cggagaccat taacactctg gctgacggag agcgtattac tgatgctgat
    10921 ccgatggaac agcccgcgtt catgcgtgac ccgcgattcc gtggttttac gggggatgat
    10981 acagaagtgc aggcccgtct tgctcgtggc aacgcgccga cggcggaaga actggtgcgt
    11041 tcccagatgg ctgaaggtga tgccggtccg acagcacagg agttaactga gcgtccacgc
    11101 cttcccgctc ccggcgatat tcatcccgga cagggatatc cgttaccagg agaagtagcg
    11161 cgtacgccgg atgaaaatca ggccggacgt ggtggtcgtt ttaccacaac cggtgaggtg
    11221 aagggccaga gtttccagaa aggacgtgcg caggcaccgg aaaatgccgc tggtcgccag
    11281 ggggaaatac tggagggcga aacagttcgt cgtggtctgc cgtcaccgga tgagcagaac
    11341 gccacagcac cagtgcgtga aggtcttcct gcacctgaaa gtcagcgtgg ggttgatatg
    11401 ccacagcctg aatcactccc tcgtatggtt cgtgactccc ttccggaact ggcacagcag
    11461 gcagaagcac gcagacagac cggggataat cgccagacca taaccgatgt tccggatact
    11521 gaggtgccgg tgccggtaga taaaccgagc actcaccagc aggcgcgtgg tgcgaaaatt
    11581 gaagactttg gcgaggagat taagggcgcg gcaaaacacc gttatgcgca gcttgctgaa
    11641 gctatgggta aaacgctgga ggatggggag tacgcgacac aaccactgag caaactgttt
    11701 ccaaagccgg attatgcccg tctggaaaaa gaaggtgtgg atagtgacac gctggcaatg
    11761 atggcgttgt atcgcagtga aattcccacc agaacaagcc gtaacatgca gaaatggatc
    11821 agcattgtta aaagcggtcg cgaagctact gccggtatgc tggaggggaa aattccggca
    11881 gcgaaactgg ctgatatgat ggacagtaag ccggggttac ggagcatgtc ggatacctgg
    11941 aaactgctcc gtacgctttc tgcattgcag attgataaag catccggcta tcgtgtcagg
    12001 tccggtgttt attcgtttgt cgggggaaaa cgttacgatc cgccgcaaat gatgtattcg
    12061 cttcgtgata gcaaggggcg cgacctcttt ttctcggaaa gccgggatga gttactgaaa
    12121 aaggcgaaag catatttcga tgaacaaggc tccagagaaa gggaaacgcc agcaacctct
    12181 gctgatgaca gaataacctt tgacgtgtac cggcataagg ccagtggcga catttttatc
    12241 ggttatggca aaaacaggca gaaactgaag ggcggttttg agtctgcccg tgatgcacat
    12301 gattatgtgc gcacacatcg tgatgagctg gttaatcagg tgaaggcgct gcgtgaagtc
    12361 tcgcgtgagg agcagcgtaa cgccaccaac cgcgatcgta ccggaccaga acgccgcaag
    12421 ggggatgttt cgccggagca gttcagtgat gcgtttggtt ttcgtggtgt gcagtttggt
    12481 aattatgtgg aaagtccgcg tcgtcaggct gatttgaacc gggcttatga ctcgctgcat
    12541 gacctggctg acgtgctgaa cgtgccgaca aaagcgcttt ctctgaacgg tcgtcttggg
    12601 cttgcttttg gtgcccgtgg taagggtaag gcggcggcac actatgagcc gggtgaggtg
    12661 gcaatcaacc tgacaaaagg taacggaccg ggggcgctgg cccacgaatg gttccattca
    12721 ctggataatt attttggtcg ttatgatgtt tccactgacg ggaagatcac gtcaggtggc
    12781 gactatatga cggaagcaca gcgtgccggg cgcgtattta aagacggcag gtatgttgat
    12841 gcggaatatc cggtacgtca ggaggtttac gacgcgttta aaggcgtgat gaaagccatt
    12901 aacagcagtg acatgttgcg tcgttctgaa cgcctggatg gcgttcgttc aaaaccgtac
    12961 tggtcaacgg atgttgaaat ggcggcgcga gcctttgagc gttatgttca ggataaagcg
    13021 cgtatggctg gcgtggagaa tgattatctg gtcaatatcc gtaaggcgga tgaccatgga
    13081 cagccggaca cctacgctta tccgacgaat gcagaactgg atggcggtat tcgtgaggca
    13141 ttcgatcact tgttccgaac actgaaaacc cgtgagacgg acaagggagt tgcgttttat
    13201 tcccgtaagg gcgtgacccg cacacctgaa ggcaatctga tttcggatgt taaccgcagt
    13261 gcggaagcca aaggcagtcc ggtcccgcag gttgaagcgg ttgcccgtgg cgtgatgagc
    13321 ggcattaagg acagtgacct gaaggtccgt gtggtgaagt cacagaaaga ggctgaagcg
    13381 ctggcgggtg aattgttcga tggttacggc agggtgcacg cattctatcg tccggataaa
    13441 cgagaaattg tcctggtggc ggataacatc cctgacgggc ggaccgttcg cgagaagctg
    13501 cgtcacgaga tcattcacca tgccatggag catgttgtca caccagcgga atatcagacg
    13561 attatcaaaa ccgtgctgaa aacccgcgac agtgataacg tcaccatccg tgaagcctgg
    13621 cgtaaggttg atgcttccta tggtaaggaa tcaccggaag tacaggcggg tgaatttctg
    13681 gcacatatgg cggagaaaca gccgaataaa ttcgtggcgg catgggagcg tgttgttgcc
    13741 ctggtcaaag gggtactgcg tcgtacgggg ttactgaagc cgacggaact gaacgatatc
    13801 agacttgttc gcgaaactat tcgcacgtta ggcctgcgtg tgcgggaagg ttacacgccg
    13861 cgtgaggatg gcgcgggcgc atcgtctcag tactcccgta gtggtaaacc tgatccgttc
    13921 aaagtgccgg aaggtgaggg cgagcgttat cgtgatgacc ttgccagaat gatgaagtcg
    13981 ttacgttctg gtgcaatgac tgcaaacatc gggcgtacgc cgccggtatt gcgccacctt
    14041 ggcgcaccag atttgccgct ggttatctcc cgcgacactg tgcggaaggc aaccaatgga
    14101 gtgaaacatg tggtgccaat ggatgttatc gagagacttc ctgagctgat gcacgatccg
    14161 gatgcaattt accgttccgc gacagaaaga aatgcggttg tgatgctgct tgatgccgtg
    14221 gataaaaatg gtgatccggt ggtgtcggca gtgcacatga aggcaacaga taaacgaata
    14281 gaaattaaca aggtggcttc tgtatatgga acaaaagggg ggatgaacaa agctaatagc
    14341 ctggataaag caggtttgac gctttaccgg aaggaaaaat taagccgcga taaccctcag
    14401 tacagtgggc ttcaattgcc caaagaggag cgttcttatc gcggctctgt agataaaata
    14461 ctctatcctg aagatattcg caatgggccg tattactccc gtaccagcag tctgacaccg
    14521 gaagagacaa ttgcatcgcg ttttgtgcgt cagatgcagg ataaattcca ggtgctgaaa
    14581 gctgttcagg agaacatccg taaaactggc ggaaaaatag acgacagtaa caacgcttat
    14641 atggcggaag aactcttcca cgggaaggcg gaaaacgacc tgaacgtgat gaaggagcgc
    14701 tacgttcagc cactggctaa attactggcg gactacaaaa ttgcgcaggc cgatctggat
    14761 gagtacctct acgcccgtca cgcgccggaa cgtaacgcgc atatcgcgaa aatcaacccg
    14821 aaaatgccgg acggcggttc ggggatgacc aacgcggaag cggcggaaat catgcagcgt
    14881 gtacgtaaca gtggcaaaca ggcacagtat gaccgtctgg cagggattat tgacgatatg
    14941 ctggcccgtc gccgtgagct tatccgtcag gccggacttg aagagaacgg tgtggtggat
    15001 gcctggcaga acgcctaccg ttactacgtt cccctgaaag gtcaggatgt tgacggtgtg
    15061 gtgtcactgc cccgtacagg taagggcttc accatcggcg gacgtgaaag caggcaggcc
    15121 atggggcgtg catcccgggc acagtctccg tccactcagg cgatacagga cctgagcgaa
    15181 tcgctgatcc gccatcgcaa aaacgaagtg ggtaacgcct tcctgaaact ggtgcaggat
    15241 aatcccgaca aggattactg gcaggtattc accgatgaca gaccggatac catgcggacg
    15301 attgcagagc gcaaggacca ggaaactggt gaaaccattc gcgaagttgt cgaacgccct
    15361 gtaccgatgg caatgatggc agaccggtac ttcaccacca aaaagaacgg caaaacgtac
    15421 tacatcaaac tccatgatcc gcgcctgatg cgtgcgatga agaatatggg accggaaacc
    15481 agtaacgccg taatccgcac gctggggaaa gttaaccgct tcctggcaac ggtgaacacg
    15541 tcgtataacc cggaattcct ggtcagtaac ttcatccgtg acgtgcagac ggcggtgatg
    15601 aacctgaagg cggagcaggg aaggagcgac ggcaaactga aagggctgga taacttatcc
    15661 gcactggctg tggtgaaaga cagccggtct gccatgtctg ccgtatacgc cagtctgcgt
    15721 ggtaaaaccc tcacgggcaa cggtgcgcag tggcagaagg tgtggaaaga gtttgttgag
    15781 gacggaggta aaaccggctg gtttaacatg ggtgatcttg aaggccagca gaaggaaatg
    15841 gatcgccttg tatcgctggc gaagggagga tggaaaggcc agagtatcgg tgcatggaat
    15901 tcgttcctta accttgtcga ggatgccaac ggggcggttg aaaacgctct gcgtctttct
    15961 gcctataaac acgcccgtga tgccggtttg tcacgccagc aggcggcgtc ccttgccaaa
    16021 aacatgacgg tgaactttaa ccgtcgtggt gagcagggag cgctgatgaa ctcgctgtac
    16081 atgttcgcca acgccagcat tcaggggacc gcaaacctgg tgagaacgct cggacatctt
    16141 aatggcgacg ggccgttact ggagcgcctt cgctggaaga atctcaatgt accgcagaaa
    16201 atcgcgcttg cagctgtggg agcgggttat ctgcttggct cgcttaaccg cagcgtggcg
    16261 ggggaggatg atgacggggt taactggtat gacaaggtgc cgtctcatgt gaaagagcgt
    16321 aacctcgtca ttatgaaatc ggtgttcggg ggcaaggccg gagagtactg gagtattcct
    16381 ctgccttacg ggtacaacgt tttcttcctg cttggtcaca cagccgaagg ggtggcagcg
    16441 ggcgacctga ccgcttcacg tgctgccggt aatgttgttg gtggtgtgct tggggcattc
    16501 agtcctgtgg gcagtgaaac gtcggaaaca ctgtccgggg cattgctgaa aaatgcagcg
    16561 ccgaccattc tgcgtccgtt tgcgaacctt gccatgaatg aaaacttcat gggggcgcag
    16621 atttaccagg agaacatacc gtttggtgca ccaaaacctg acagccagct gggaagacgt
    16681 tcaacgccag aagcgtacaa ggcgtttgca tcctggctga atgcgttctc aggtggcagc
    16741 cagtatcgtc ccggcgcggt ggatatcaca ccggaatcgc tgaaattctg gattgactat
    16801 atctccggag ggacagggcg cttcatttcc aaaaccacgg atgcggcggt gaaatcgctg
    16861 aatggtattg atataccgga acagcaggtg cccttcctgg ggaaaatttc aggtgaggtg
    16921 atgccgtatg ctgaccagca gaagatgtac gaccggatga cggaggtcgc gcagtatcac
    16981 gcagagctga agagtctgac cggtgcagaa agaacggcgt tcattgacga gaacaacgga
    17041 aaattgtcga tgaacgggct tatgcaggat acccggaaga gactgaagga tttgcgtaaa
    17101 cagcgtgatg ccatttacgc cgacagtact ctcagtctgg cgcaacagtc ggcgatggtg
    17161 aaatcggtag agcgggatat gaaaattgcc gtggatcggt ttaaccgcga gtacaacaaa
    17221 aaagtgggag tggagtaaag aacatgcccc gtacggaagt gcggggcatg tttaacggga
    17281 tgtgtacata aatgggtact gatatgacga ttatttcggt actttctgta tttaaatcaa
    17341 tgacttaata cttatattca ttatatccat ttaactaaga ggacaatgcg gcatgagtat
    17401 acccgctaat ggactgcggg gtaagtacgc tgccgctcga ttgcttaaac cctcgccatt
    17461 tatgccgggt ttttatcatt tttcttaatg ttttccgcac gttctgcttt ttggcgtgct
    17521 tctgctttgc gcttgttgct catgtcgtta cggatctgtg catgactcat taacgcgaag
    17581 ataaaggtgc cgccgcagat gttccccgct aaagtaggta gtgcgaaggg ccagatgaaa
    17641 tcgctccagt gcagcgtacc gttaaacacc agatagagga tttcaacaga accgacaacg
    17701 atgtgagtgg tgtcacccag ggcaataagc caggtcatca atataatcac cacaatcttt
    17761 gccgcacccg ctgcaggaaa catccatacc atagtggcga tcagccagcc ggaaatgatc
    17821 gcgttggcaa acatctcgct gggggtgttc ttcatcacat ccatgccgat tttgacaaat
    17881 gcatcgcgag tttcttcatt gaagattggc atatattcaa atgcccacgc cgcaatacct
    17941 gtcccgagaa tattacccag cagcacgacg ccccataacc gcataagtaa gccaacgttg
    18001 ctcattgtcg gtttttgcat gacgggtagt accgcagtca cggtgttttc ggtaaataat
    18061 tgctggcggg ccataatgac gataataaaa ccaaaggtat aaccgagatt ctccagcaag
    18121 aagctgcccg gcacaccttc cagttcgaca tgaaatatcc cttttgccag taatgaagcg
    18181 cccatcgaca gacccgccgc aatggctgac cacagtagtg ccattgcgtc gcgttccagc
    18241 tctttttcac catcctggcg gatatgctca tgaattgcca tcgcccggga ggggagtcga
    18301 tcttcatcta tttctatttt tttgccacgc tctttttctt cgctctcaac ttcaatttcg
    18361 tcgctgtgtt gatcaatttt gtcgttgtcc atggtctctt ctcttgaatt agcacgtata
    18421 gctaaagcgt agcggctttt ttgctcgcaa ctggcgggag ttactttgaa aatgtagaaa
    18481 aggccgcgtt tgcctttttc tgtttctata gaatcaagta gcctacaggg cggcgattac
    18541 caggctatga tcaaatcagc aaatcagggc gtctggacat cagttgacgt gctgttacaa
    18601 tcgcccacac ctaaacaggc ggatacggta tcgttccgtc atggatggca aactgcataa
    18661 gccataaaaa aacagggaga catttatgaa gcttcgcctg tcggcgcttg ctctgggaac
    18721 tacgcttctg gtggggtgtg cgagttccgg tacagatcag caagggcgtt ctgacccgtt
    18781 agaagggttc aaccgcacca tgtacaactt taacttcaat gtattagacc cgtatattgt
    18841 tcgaccggtc gctgtcgcct ggcgtgatta tgttccgcaa ccggcgcgta acggtttgag
    18901 caattttacc ggcaaccttg aagaacctgc ggtgatggtt aactacttct tgcagggcga
    18961 cccttatcag gggatggtcc actttacccg ctttttcctg aacaccattt tggggatggg
    19021 cggctttatt gatgttgcag ggatggcgaa cccgaaactg caacggactg aacctcaccg
    19081 cttcggtagt acgcttggtc attatggcgt gggttatggc ccgtacgttc aattaccgtt
    19141 ctacggtagc ttcacgctgc gtgatgacgg tggtgatatg gcggatggtc tttacccggt
    19201 tctttcctgg ctgacctggc cgatgtctgt gggtaaatgg acgcttgaag gtatcgaaac
    19261 ccgcgcacag ctgctggatt ccgacggtct gctgcgtcag tcgtccgatc cttatattat
    19321 ggtgcgcgaa gcgtacttcc agcgtcatga tttcatcgct aatggcggcg aactcaaacc
    19381 gcaggaaaat ccgaacgcgc aagcgattca ggatgattta aaagatatcg attctgaata
    19441 agaaacaaat aaaaaaggtg agtcgcaaaa ctcacctttt ttgttgttat ccctcaatta
    19501 ccacaagtaa aa
//
LOCUS       DBFIFI010000073        18906 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.073, whole genome shotgun sequence.
ACCESSION   DBFIFI010000073 DBFIFI010000000
VERSION     DBFIFI010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18906
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.073"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(23..646)
                     /gene="rsmG"
                     /locus_tag="N7B39_002482"
     CDS             complement(23..646)
                     /gene="rsmG"
                     /locus_tag="N7B39_002482"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HGU2422447.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(710..2599)
                     /gene="mnmG"
                     /locus_tag="N7B39_002483"
     CDS             complement(710..2599)
                     /gene="mnmG"
                     /locus_tag="N7B39_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HGU2422448.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSDDKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(2978..3421)
                     /gene="mioC"
                     /locus_tag="N7B39_002484"
     CDS             complement(2978..3421)
                     /gene="mioC"
                     /locus_tag="N7B39_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HGU2422449.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(3511..3969)
                     /gene="asnC"
                     /locus_tag="N7B39_002485"
     CDS             complement(3511..3969)
                     /gene="asnC"
                     /locus_tag="N7B39_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HGU2422450.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4121..5113
                     /gene="asnA"
                     /locus_tag="N7B39_002486"
     CDS             4121..5113
                     /gene="asnA"
                     /locus_tag="N7B39_002486"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HGU2422451.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(5118..6569)
                     /gene="viaA"
                     /locus_tag="N7B39_002487"
     CDS             complement(5118..6569)
                     /gene="viaA"
                     /locus_tag="N7B39_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HGU2422452.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(6563..8059)
                     /gene="ravA"
                     /locus_tag="N7B39_002488"
     CDS             complement(6563..8059)
                     /gene="ravA"
                     /locus_tag="N7B39_002488"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HGU2422453.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPASLQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHNMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQRCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            8282..10150
                     /gene="kup"
                     /locus_tag="N7B39_002489"
     CDS             8282..10150
                     /gene="kup"
                     /locus_tag="N7B39_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HGU2422454.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANQEVLYALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGGEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            10317..10736
                     /gene="rbsD"
                     /locus_tag="N7B39_002490"
     CDS             10317..10736
                     /gene="rbsD"
                     /locus_tag="N7B39_002490"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HGU2422455.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKHHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            10744..12249
                     /gene="rbsA"
                     /locus_tag="N7B39_002491"
     CDS             10744..12249
                     /gene="rbsA"
                     /locus_tag="N7B39_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HGU2422456.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFESCDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            12254..13219
                     /gene="rbsC"
                     /locus_tag="N7B39_002492"
     CDS             12254..13219
                     /gene="rbsC"
                     /locus_tag="N7B39_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HGU2422457.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAMVILLAVLVDNKKQ"
     gene            13244..14134
                     /gene="rbsB"
                     /locus_tag="N7B39_002493"
     CDS             13244..14134
                     /gene="rbsB"
                     /locus_tag="N7B39_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HGU2422458.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            14260..15189
                     /gene="rbsK"
                     /locus_tag="N7B39_002494"
     CDS             14260..15189
                     /gene="rbsK"
                     /locus_tag="N7B39_002494"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HGU2422459.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            15193..16185
                     /gene="rbsR"
                     /locus_tag="N7B39_002495"
     CDS             15193..16185
                     /gene="rbsR"
                     /locus_tag="N7B39_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HGU2422460.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(16151..17578)
                     /gene="mdtD"
                     /locus_tag="N7B39_002496"
     CDS             complement(16151..17578)
                     /gene="mdtD"
                     /locus_tag="N7B39_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HGU2422461.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPILGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNHVPSESE"
     gene            complement(17601..18293)
                     /gene="yieP"
                     /locus_tag="N7B39_002497"
     CDS             complement(17601..18293)
                     /gene="yieP"
                     /locus_tag="N7B39_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HGU2422462.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
     gene            18774..>18906
                     /locus_tag="N7B39_002498"
     rRNA            18774..>18906
                     /locus_tag="N7B39_002498"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         4577 a         4667 c         4935 g         4727 t
ORIGIN      
        1 tttatgattt ttttgataaa aattaaattt tatttgcttt aatcaccacc agatgacgtt
       61 cgccatccag ggctggaacc tgaagtttaa ccactgattc gacctgatat tcttcgggca
      121 acaaagcgat ttcatcttcc ggcatttgcc ctttcagcgc gtagaaacgg ccttgctcac
      181 caggaagatg gtggcaccag ctcaccatat cgttcagaga ggcaaaagcg cggctaatta
      241 cgccatcaaa tggcggctct gaaggaaact cttctaccct gctctgtact ggctcaatat
      301 tctccagttt aagctcatgt tgcacctgac gaaggaaacg cacgcgttta ccaaggctat
      361 ccaacagagt gaaatgggct tcaggacgca cgatagagag tggaatgcca ggcagtccag
      421 gaccggtgcc gacatcgata aaccgttcac cttgcagata cggtgccacc acaatgctat
      481 cgagaatatg gcgtaccagc atctcattag gatcgcggac cgaagtcagg ttgtacgctt
      541 tgttccattt atgcagcata ttcacgtagg caataagctg gtttttctgg tgatcggtaa
      601 gcgaaatacc tgcgtctttc agcagtaagg agagtttgtt gagcacggtg attacctgtt
      661 cttgatgcgt tgcctggtaa gcgggggctt accaggcatt tttaatgcgt tatgcgctac
      721 gacgcagcat accctgtttt ttcagccaca ccagcagaat ggagatggcc gcaggcgtga
      781 cgccagaaat acgtgaagcc tggccgatag aggccggttt gtgatcgtta agtttggcga
      841 tcacttcgtt agaaagaccg gatacctggc ggtaatccag tgtcgcgggt agcaaggtgt
      901 tctcgttacg cagctgcttt tcgatctcat cttgctggcg cgcgatataa ccttcgtatt
      961 taacctgaat ctcaacctgt tccgccgcct gttcgtctgt caacgcaggg gcaaacggcg
     1021 tcagcgtggt taatttttca taagtcattt ccggacgacg cagcagatct tcaccactgg
     1081 cttcacggga aagcggcgca gtcaggtgag cattcacttc ggctgcagct tccgccgacg
     1141 gggttaccca ggtcgatttc agacgctgac gctcacgctc gatattctca agtttctcgt
     1201 taaagcgcgc ccaacgttcg tcatccacca ggcccagttc acgaccgatt tcagtcaaac
     1261 gcagatccgc attatcttcg cgcagcatca gacgatattc tgcgcgcgag gtaaacatac
     1321 gatacggttc tttggttcct aaagtgcaca ggtcatcaac cagtacgccg agataggcct
     1381 gagaacgcgc cggagcccaa ccttctttgt catcagacag acgggcagcg ttaagaccgg
     1441 ccagcaaacc ttgcgcagcg gcttcttcgt aaccggtagt gccgttaatc tgaccagcaa
     1501 agaacagccc ctggataaac ttgctctcca gcgtcggttt caggtcgcga ggatcgaaga
     1561 agtcatactc aatggcataa cccggacgca cgatcttcgc gttttccatc ccctgcatag
     1621 agcggacgat ttgcatctgc acatcgaacg gcaggctggt ggagataccg ttcggataaa
     1681 tttcgttaga agtcagccct tccggttcaa ggaagatctg atgctgattt ctgtcggcga
     1741 agcgcatgac tttgtcttcg atcgacgggc agtagcgtgg gccgacacct tcgatcaccc
     1801 ctgcgtacat tgggctacga tcgaggttac tgcggatcac atcatgggtt ttctcgttgg
     1861 tatgggtgat ataacacggc acctgctgtg gatgctggga cgcattgccc ataaaagaga
     1921 ataccggcat tgggttatcg ccatgctgtt gcgcaagcac gctaaagtcg atggttcgag
     1981 catcaatacg cggtggtgtc ccggttttca gacgaccaac gcgcagcgga agttcacgca
     2041 aacggcgaga aagcggaatg gacggcggat caccagcacg gccaccgctg tagttatcca
     2101 gaccgatatg aattttaccg tcgaggaacg tcccaacggt gagcacgacg gctttggcac
     2161 ggaacttcag tcccatttgg gtaacagcac cgaccacacg atcgttttcg acaataagat
     2221 cttcaaccgc ctgctggaag atcatcaggt tcggttggtt ctccagcgcc gtacgtaccg
     2281 cctgacggta gagcacacga tccgcctgag ctcgggtagc gcgaactgcc ggtcccttgc
     2341 ttgcgtttag tatcctaaac tggatacccg cctgatcgat cgctttcgcc atcagaccgc
     2401 cgagtgcatc cacttctttt accagatgtc ccttcccaat accgccgatc gccgggttgc
     2461 agctcatctg ccccagagtg tcgatattgt gtgtcaaaag cagagtctgt tgacccatac
     2521 gcgccgcagc catcgcggcc tcggtgcctg catgaccccc gccaatgatg atgacgtcaa
     2581 aaggatccgg ataaaacatg gtgattgcct cgcataacgc ggtatgaaaa tggattgaag
     2641 cccgggccgt ggattctact caactttgtc ggcttgagaa agacctggga tcctgggtat
     2701 taaaaagaag atctatttat ttagagatct gttctattgt tatctcttat taggatcgca
     2761 ctgccctgtg gataacaagg atccggcttt taagatcaac aacctggaaa ggatcattaa
     2821 ctgtgaatga tcggtgatcc tggaccgtat aagctgggat cagaatgagg ggttatacac
     2881 aactcaaaaa ctgaacaacg gttgttcttt ggataactac cggttgatcc aagcttcctg
     2941 acagagttat ccacagtaga tcgcacgatc tgtatactta tttgagtaaa ttaatccacg
     3001 atcccagcca ttcttctgcc ggatcttccg gaatgtcgtg atcaagaatg ttgatcttca
     3061 gtgtttcgcc cgtctgtttt gcaccggaat ttttgagttc agcctcgagt ttatcgatag
     3121 ccccacaaaa tgtgtcatat tcacgactac caataccgat tgcgccaaag cggaccgcag
     3181 aaagatcggg cttctgttcc tgcaatgctt catagaaagg agaaaggttg tccggaatat
     3241 ctccggcacc gtgggtggag ctgataacca gccagatccc cgaggcagat aaatcttcca
     3301 acagcggacc gtgcagcgtt tcggtggtaa aacccgcctc ttccagcttt tcagccaggt
     3361 gttctgctac atattcggca ccgccgaggg tgctgccgct gataagagtt atatctgcca
     3421 taaaccgcca cctttattaa gagtggcgta ttgtacgctg tgaacgcgtt gggatctacc
     3481 tgtggaaaag tatgggatta aaaaagccga tcagggcttg atggtacgca tgatcgggtt
     3541 ctgcaggacg atcagtgtct cggtggactg aatttcatca attgtttgga tcttgttgat
     3601 aagtacatgc tggagagcat cgatcgaacg gcacatcact tttataaaga tgctgtagtg
     3661 gccggtggtg tagtacgcct cggtcacttc atcgaggctt tccagctttg ccagcgcgga
     3721 agggtagtct ttggcgctct ttaatataat gccgataaag cagcctacgt cataaccgag
     3781 ctgcttcggg ctgacatcaa tacgcgcccc ggtaatgatc cccgcctgct tcattttctc
     3841 tactcgaacg tgaatcgtcc ccggactgac gccaaattgt ttcgccagtt cggcgtaagc
     3901 ggtgcgcgca ttgcccatta atgcttccag gatgccacgg tccagattgt cgatcagata
     3961 attttccata ggattttctt atgcggattg atgattcatt ctattttagc cttctttttt
     4021 aatgaatcaa aagtgagtta ggctttttat tgaatgatta ttgcatgtgt gtcggttttt
     4081 gttgcttaat cataagcaac aggacgcagg agtataaaaa atgaaaaccg cttacattgc
     4141 caaacaacgc caaattagct tcgtgaaatc tcacttttct cgtcaactgg aagaacgtct
     4201 ggggctgatc gaagtccagg cgccgattct tagccgtgtg ggggatggca cgcaggataa
     4261 cttgtcgggc tgtgaaaaag cggtacaggt aaaagtgaaa gctctgcctg atgcccagtt
     4321 cgaagtggtt cattcactgg cgaagtggaa acgtcagacc ttagggcaac acgacttcag
     4381 cgcgggcgaa gggctgtaca cgcacatgaa agcccttcgc cccgatgaag accgtctttc
     4441 tccgttgcac tcggtctatg ttgaccagtg ggactgggaa cgcgtaatgg gcgacggtga
     4501 gcgtcaattc tcgactctga aaagcacggt agaggcgatt tgggcgggaa ttaaagcaac
     4561 cgaagctgcg gttagcgaag agtttggcct ggcaccgttc ctgccggatc agatccactt
     4621 cgtacacagc caggagttac tgtctcgtta tccggatctt gatgccaaag ggcgtgagcg
     4681 ggcgatagcg aaagatcttg gtgcggtatt ccttgtcggg attggcggca agctgagcga
     4741 tggtcatcgc cacgacgtgc gcgcaccgga ttatgatgac tggagcaccc cgtcagagct
     4801 gggccatgcg ggtctgaacg gcgatattct ggtgtggaac cctgtactgg aagatgcgtt
     4861 tgagctttct tccatgggga tccgcgtgga tgccgacacg ttgaagcatc agttggcgct
     4921 gaccggtgat gaagatcgtc tgcagctgga atggcatcag gcgttgctgc gcggtgaaat
     4981 gccgcagacc atcggcggcg gtatcggcca gtctcgtttg accatgctgc tgctgcaact
     5041 gccgcatatc ggccaggtcc agtgtggagt atggccagct gctgttcgcg agagcgtccc
     5101 ttctctgctg taataattta tcgccgccag cgtctgagca ggcggcttcg catcccggta
     5161 tcaaagcgcc agatatgatc gaaaatgcgc atgatgccgg gtttgccgtg tgccgacatc
     5221 gccacggcat gaaagcgatg ctgatgtacc cgctgcagct ctttcacttt actcgtcacg
     5281 tcgtcaggca accgctgggc gataaaatca gaaatcacca ccgcatcggc atcaaaccat
     5341 tccctgcttt gcaagcgttc cataatggcg cgaaaacaac tggcaagatc ggtgccgcca
     5401 cgaaactgct ggcttaaaaa acggattgct tgttcgatgc cttgtgggcc tgaaagctca
     5461 taacggacga tctcggtgga aaatagcata atataacagc gccggttttc agcgagagca
     5521 atgcgcatca aagccaggca gaacgctttc gcacactgtt cattaaagcc gcccattgag
     5581 ccggaagtat ccacgcagac aataaacggt ccgcgcggct gttcgtcgta atctttatgc
     5641 accaccgggc gttcgatcac tttttcacgc cacgactcac cgtgcaggcg ataggtgagc
     5701 aactgttttt ccaccagccg acggtaaaac tcatactcca gttccgttat ccctagtgtc
     5761 gccagttctg gcggcagaag acgtaaaata tcatcgcttt gttgcagacc atcaacctgc
     5821 tcaggaaccg tcgccggttc gcgcaccatg gtgcggaagg tttccatctg cgcatcgttg
     5881 cgcggtattg atttggcttc ccgggaacgc cccagttgtt ctgccaggcg tttcagttcc
     5941 ggctgttcgt taagaaattc accgtatttc acaatcaact gatagtcgcc acgtttaagc
     6001 tgaccggcgc tcatatccca cagacgacca gctgcggtat tgttatctgc gagaatcggt
     6061 tcaagttgtc cgctcagcgt catgcgttcc tgaacttcac tcaacagttg ttcgcgttct
     6121 tcttctaata gctgttgatt taacgttgtt gcttgcacga tcagacttaa acgccagcgc
     6181 tggagaaaaa gcgtgtgtaa cgctgaagtg atcgtgctgt tagcatcgac caactgtcgg
     6241 gcttgttctg cccatgggga attcagacga tgcagtaagt ccaggatctg tggtagctgc
     6301 acaataaact gcggcgtgga gaggagctgg ctttgctgat agcacatcac ctcttcggtg
     6361 agttccggcg ggactcgggc atctttcagc cgactgcgca gcgcctcacg ccagcgggga
     6421 acatcatcag tgattgccgc cttcagtcgt gggaattttt caaagaagac tgccagctgc
     6481 ggtgaggcca gcagcgcgat gatcatctct tcgatcaatc cctcttcgct gacggccagc
     6541 atcacattaa gcgtatccag cgttagcatt gttgtgcctg gcgaatctgt gcgccgacat
     6601 cctgtaggct ggcttcaatg cgacctaacc agtcgccagg gataaacaga catctttgct
     6661 gttcgctgaa taaagcgtgt tgcttgcgcc agtcgctttc aagctcttcc aattgttgtt
     6721 ttatttcacc tggcagacct tcagccgatg aaccgggaag tgccagcgta ctgccttgta
     6781 aacttacatc gcgtacaaca agatgttggg cgctatcaac ttccagattc agtttctggg
     6841 caaagccgat accgttcagt ttgccgcgaa tttcaccacc tttgctcagc cactgttcca
     6901 gcgcgctacg ctcaaaggag atatgaacca cttccatatt atgcaatttt aacggttttt
     6961 gcagcagcag agtcagagtg gaagcagtaa cgttaacagg aagttgatac tgctgacgac
     7021 ggctgaaaat gccgcccaga cgaattaccg ttaaggctgt tttatcgctt tgttgctgct
     7081 ggagttgcag gtgacgttgc acaatcgcgc ccaggcgggt caacatccct tgctgttgcc
     7141 aggcatgacc ggtcatcaat acatcaattt gttgttgtat caaattcagg ctttgcgcgt
     7201 cataccacag gcaatctttc agcaaaatga gatctaccgg ggcaacagca ctgcgaccgc
     7261 taaaaaaggc gctggcctgc aataatcgaa tcgctttttt ccagcgacga tccgagacat
     7321 aaggcgcatc cggtaattta tccagttgct ggcgcagcat aaaaatcagc tcaaatacat
     7381 gatcgggcag cgtaatttca ccaatctctt tttgccagcg ttcatattct tcatctgtga
     7441 cctgcaagga ggctggaacc ggattgtcat tttcatcctg ttgactggtc agcatggagc
     7501 ggaaattcgc tttatcctgc actttatcta accacagacg aatcagcatg cggtcatata
     7561 acgcttccag actgctgtct gcttccggca gctcgttgga ggccgccacc agcaggcgca
     7621 tcgggatttt ttctacgtgt gcaccgttgc ggaactggcg ctcgttaatg gcggtgagca
     7681 gggtattaag aattgccgga cccgctttcc agatctcatc cagaaagacg atttctgctt
     7741 ccggcaggta accgctggtt aaacgttcat agcgcccttc atcttttagc gcctgaatag
     7801 aaaggggacc aaaaacttct tccggcgtgg agaagcgggt catcagatat tcaaacgcgc
     7861 gggcattctg aaaggcgaat tttaagcgcc gggcgatcaa acttttggca atacctggcg
     7921 ggccaaggag gaacacactt tcaccactta atgccgctaa taaacacaag cggatggcgt
     7981 ggctacgttc ataaagcccc ttttccaacg aactgctcag gcgggaaatt ctttccgcta
     8041 ataaatgagg gtgagccata atgaagtggc gtcctttcgt caaaagttct gcgtaaattg
     8101 cgagtataga cgtttcttgc tggtggctaa aatagtctca aagggggggt atttttcttt
     8161 gagccaggtt aatgtggccg catttaggag tacgattttg ccgttaatcg tgcatactgt
     8221 gcgctttttt gtgggccaag ggactaagca cacatttcat atttcaacga aagactagtc
     8281 tatgagcact gataataagc aatcattgcc cgcgattacc ctcgcggcga ttggagttgt
     8341 ctacggcgat attggtacca gcccgttata tacacttcgt gaatgtttgt ccggccagtt
     8401 tggttttggc gttgaacgcg atgccgtgtt tggcttttta tcgctgatct tctggctgct
     8461 aatctttgtg gtttccatta aatatctcac cttcgtgatg cgggcagata acgccggtga
     8521 aggggggatc ctgacgttga tgtcgcttgc cgggcgtaat acgtcggcgc gaaccacatc
     8581 aatgctggtg attatggggc taatcggcgg cagctttttc tatggtgaag tcgtcataac
     8641 acccgctatt tcggtgatgt cagccattga aggtctggaa atcgtcgccc cgcagctgga
     8701 tacctggata gttcccctct caattatcgt tctcacttta ttatttatga ttcaaaagca
     8761 tggcacggct atggtcggta agctgtttgc gccgatcatg ctgacctggt ttttgattct
     8821 ggcaggactg gggttacgta gcattattgc taaccaggaa gtgctgtatg cgctgaatcc
     8881 gatgtgggcg gtgcatttct tccttgaata caaaacggtt tcttttattg cattaggggc
     8941 agtggtgctg tcgattacgg ggggcgaggc gctgtatgct gatatggggc actttggtaa
     9001 gttccctatt cgcctggcgt ggttcaccgt cgtattacct tccttgaccc ttaattactt
     9061 cggtcaggga gcgctgttgt taaagaatcc ggaagcgatt aagaacccgt tcttcctttt
     9121 ggcaccggac tgggcgctga tcccgctgct gatcatcgcc gcactggcga cggtaatcgc
     9181 ctcgcaggcg gttatctctg gcgtcttttc attgacgcgt caggcggtac gtctgggata
     9241 tttgtcgccg atgcgcatta ttcacacctc cgaaatggag tcagggcaaa tctatattcc
     9301 gtttgtgaac tggatgctct atgtcgcggt cgtgattgtg attgtcagct ttgagcactc
     9361 cagcaacctg gcggcggcgt acgggattgc ggtgaccgga accatggtgc tgacgtctat
     9421 tctctcgact accgtggcac gtcagaactg gcactggaat aagtattttg ttgcgctgat
     9481 cttgattgct ttcctttgtg tcgatattcc attgttcacc gctaacctcg ataaactgct
     9541 ctccggcggc tggttgccat tgagcctcgg tacggtgatg tttatcgtga tgaccacctg
     9601 gaagagtgag cgtttccgct tgctgcggcg gatgcatgaa catggtaact ctctggaagc
     9661 gatgattgct tcgctggaga aatcaccgcc tgttcgcgtg cccgggaccg cggtatatat
     9721 gtcgcgtgca atcaacgtca ttccctttgc gctgatgcat aaccttaaac ataacaaggt
     9781 attgcatgag cgggtgattc tgttaactct gcgcaccgaa gacgctccat atgtccataa
     9841 cgtccgtcgg gtacagattg aacaactgtc gcccactttc tggcgcgtgg tggcaagtta
     9901 tggttggcga gaaacgccaa acgtggaaga agttttccac cgctgcggtc tggaaggatt
     9961 aagttgccgg atgatggaaa cctccttctt tatgtcgcat gagtcgttga tcctcggcaa
    10021 acgcccgtgg tatttgcgtc tgcgcggcaa gctgtacttg ctgctgcaac gtaatgcgct
    10081 gcgtgcacca gatcaatttg aaatcccgcc aaacagggtt atcgaactgg gtactcaggt
    10141 cgaaatctaa cgccagacgc ctcctttctt cataaggggg cgtttttgtt ttcatggtta
    10201 atcaccatgt aaaacgtttc gaggttgatc acatttccgt aacgtcacga tggttttccc
    10261 aactcagtca ggattaaact gcgggtcagc gaaacgtttc gctgatggag aaaaaaatga
    10321 aaaaaggcac cgttcttaat tctgatattt catcggtgat ctcccgtctg ggacataccg
    10381 atacgctggt ggtgtgtgat gctggtttac ccatccccaa aagtacaacg cgtatcgata
    10441 tggcattaac ccagggtgta ccttctttta tgcaggtgct gggcgtcgtc acaaatgaaa
    10501 tgcaggtcga ggctgccatt atcgcggaag agatcaaaca ccataatccg caactccacg
    10561 aaacgttgct cactcacctt gagcagctgc aaaaacacca gggaaatacc attgaaattc
    10621 gttacaccac gcatgaacaa ttcaaacaac aaaccgcaga aagtcaggcg gtaattcgca
    10681 gcggagaatg ttctccgtat gcgaatatca ttctctgtgc tggcgtgacg ttctgaggcc
    10741 gtcatggaag cattacttca gcttaaaggc atcgataaag ccttcccggg cgtaaaagcc
    10801 ctctcgggcg cagcgttaaa tgtctatccg ggccgcgtga tggcgctggt gggcgaaaac
    10861 ggcgcgggta aatccaccat gatgaaagtg cttactggca tctatactcg cgatgccggt
    10921 acgcttttat ggctggggaa agaaacgaca tttaccgggc cgaaatcttc ccaggaagcc
    10981 gggattggga ttatccatca ggaactgaac ctgatcccgc agttaaccat tgccgaaaac
    11041 attttcctcg gtcgtgagtt tgttaatcgc tttggcaaaa ttgactggaa aaccatgtat
    11101 gccgaagcgg ataaattgct ggctaaactt aacctgcgct ttaaaagcga caagctggtg
    11161 ggcgatcttt ccatcggtga ccagcaaatg gttgaaatag ccaaagtgct gagctttgag
    11221 tcgaaagtca tcattatgga tgaaccgacc gatgcgctga ccgataccga aaccgaatcc
    11281 ctgttccgcg tcatccgcga gctgaaatcg caaggccgcg gtattgtcta tatctcccac
    11341 cgcatgaaag aaatcttcga gagttgcgat gacgttaccg tttttcgtga cggacaattt
    11401 attgctgagc gcgaagtggc atcactgacc gaagattcgc tgattgagat gatggtgggt
    11461 cgcaagctgg aagatcaata tccgcacctg gacaaagcgc cgggagatat ccgcctgaaa
    11521 gtcgataatc tctgcggacc tggcgttaac gatgtctctt ttactttacg caaaggcgaa
    11581 attcttggcg tctctggttt gatgggcgca ggtcgtaccg aactgatgaa agtgctctac
    11641 ggcgcactac cgcgcaccag cggttacgtc accctggatg ggcatgaagt cgttacccgt
    11701 tcaccgcagg atggcctggc aaacggcatt gtgtatatct ccgaagaccg taaacgtgac
    11761 ggtttagtgt tgggcatgtc agtaaaagag aacatgtcgc tgacagcgct gcgctacttc
    11821 agccgcgctg gcggcagttt gaagcatgcc gatgaacagc aggctgtgag tgattttatt
    11881 cgcctgttca atgtgaaaac gccgtcgatg gagcaggcaa ttggtctgct ttccggtggc
    11941 aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac gccccaaagt gttgatcctt
    12001 gatgaaccta cccgtggcgt agatgtcggc gcgaaaaaag agatctatca actgattaac
    12061 cagttcaaag ccgatggctt gagcatcatt ctggtgtcat cggagatgcc agaagtatta
    12121 ggcatgagcg atcgcatcat cgtcatgcat gaagggcatc tcagcgggga atttactcgt
    12181 gagcaggcca cccaggaagt gttaatggct gccgctgtgg gcaagcttaa tcgcgtgaat
    12241 caggagtaaa aaaatgacaa cccagactgt ctctggtcgc cgttatttca cgaaagcgtg
    12301 gctgatggag cagaaatcgc ttatcgctct gctggtgctg atcgcgattg tctcgacgtt
    12361 aagcccgaac tttttcacca tcaataactt attcaatatt ctccagcaaa cctcagtgaa
    12421 cgccattatg gcggtcggga tgacgctggt gatcctgacg tcgggcatcg acttgtcggt
    12481 aggttctctg ttggcgctaa ccggcgcagt tgctgcatct atcgtcggca ttgaagtcaa
    12541 tgcgctggtg gctgtcgctg ccgctctcgc gttaggtgcc gcaattggtg cggtaaccgg
    12601 agtgattgta gcgaaaggtc gcgtccaggc gtttatcgct acgctggtta tgatgctttt
    12661 actgcgcggc gtgaccatgg tttataccaa cggtagccca gtgaataccg gctttactga
    12721 gaacgccgat ctgtttggct ggtttggtat tggtcgtccg ctgggcgtac cgacgccagt
    12781 ctggatcatg gggattgtct tcctcgcggc ctggtacatg ctgcatcaca cgcgtctggg
    12841 gcgttacatc tacgcgctgg gcggcaacga agcggcaacg cgtctttctg gtatcaacgt
    12901 caataaaatc aaaatcatcg tctattctct ttgtggtctg ctggcatcac tggccgggat
    12961 cattgaagtg gcgcgtctct cctccgcaca acccacggcg gggactggct atgagctgga
    13021 tgctattgct gcggtggttc tgggcggtac gagtctggcg ggcggaaaag gtcgcattgt
    13081 tgggacgttg atcggcgcat taattcttgg cttccttaat aatggattga atttgttagg
    13141 tgtttcctcc tattaccaga tgatcgtcaa agcgatggtg attttgctgg cggtgctggt
    13201 agacaacaaa aagcagtaat aacgactaca ggacatcttg aatatgaaca tgaaaaaact
    13261 ggctaccctg gtttccgctg ttgcgctaag cgccaccgtc agtgcgaatg cgatggcaaa
    13321 agacaccatc gcgctggtgg tctccacgct taacaacccg ttctttgtat cgctgaaaga
    13381 tggcgcgcag aaagaggcgg ataaacttgg ctataacctg gtggtgctgg actcccagaa
    13441 caacccggcg aaagagctgg cgaacgtgca ggacttaacc gttcgcggca ccaaaattct
    13501 gctgattaac ccgaccgact ccgacgcagt aggtaatgct gtgaagatgg ctaaccaggc
    13561 gaacatcccg gttatcactc ttgaccgcca ggcaacgaaa ggtgaagtgg tgagccacat
    13621 tgcttctgat aacgtactgg gtggcaaaat cgccggtgat tacatcgcga agaaagcggg
    13681 tgaaggtgcc aaagttatcg agctgcaagg cattgctggt acatccgcag cccgtgaacg
    13741 tggcgaaggc ttccagcagg ccgttgctgc tcacaagttt aatgttcttg ccagccagcc
    13801 agcagatttt gatcgcacca aaggtttgaa cgtaatgcag aacctgttga ccgctcatcc
    13861 ggatgttcag gctgtattcg cgcagaatga tgaaatggcg ctgggcgcgc tgcgcgcact
    13921 gcaaactgcc ggtaaatcgg atgtgatggt cgtcggattt gacggtacac cggatggcga
    13981 aaaagcggtg aatgatggca aactagcagc gactatcgct cagctacccg atcagattgg
    14041 cgcgaaaggc gttgaaaccg cagataaagt gctgaaaggc gagaaagttc aggctaagta
    14101 tccggttgat ctgaaactgg ttgttaagca gtagttttaa tcaggttgta tgacctgatg
    14161 gtgacataaa tacgtcatcg acagatgaac gtgtaatata aagaaaagca gggcacgcgc
    14221 caccctaaca cggtggcgca ttttatggac atcccgaata tgcaaaacgc aggcagcctc
    14281 gttgttcttg gcagcattaa tgctgaccac attcttaatc ttcaatcttt tcctactcca
    14341 ggcgaaaccg taaccggtaa ccactatcag gttgcatttg gcggcaaagg cgcgaatcag
    14401 gctgtggctg ctgggcgtag cggtgcgaat atcgcgttta ttgcctgtac tggcgatgac
    14461 agcattggcg agagcgttcg ccagcagctc gccactgata acatcgatat tactccggtc
    14521 agcgtgatca aaggcgaatc aacaggtgtg gcgctgattt ttgttaatgg cgaaggtgag
    14581 aatgtcatcg gtattcatgc cggcgctaat gctgcccttt ccccggcgct ggtggaagcg
    14641 caacgtgagc gtattgccaa cgcgtcagca ttattaatgc agctggaatc accactcgaa
    14701 agtgtgatgg cagcggcgaa aatcgcccat caaaataaga ctatcgttgc gcttaacccg
    14761 gctccggctc gcgaacttcc tgacgaactg ctggcgctgg tggacattat tacgccaaac
    14821 gaaacggaag cagaaaagct caccggtatt cgtgttgaaa atgatgaaga tgcagcgaag
    14881 gcggcgcagg tactgcatga aaaaggtatc cgtactgtac tgattacttt aggaagtcgt
    14941 ggtgtatggg ctagcgtgaa tggtgaaggt cagcgcgttc ctggattccg ggtgcaggct
    15001 gtcgatacca ttgctgccgg agataccttt aacggcgcgt taatcacggc gttgctggaa
    15061 gaaaaaccat tgccagaggc gattcggttt gcccatgctg ccgctgcgat tgccgtaaca
    15121 cgtaaaggcg cacaaccttc cgtaccgtgg cgtgaagaga tcgacgcatt tttagacagg
    15181 cagaggtgac gcttggctac aatgaaagat gttgcccgcc tggcgggcgt ttctacctca
    15241 acagtttctc acgttatcaa taaagatcgc ttcgtcagtg aagcgattac cgccaaagtt
    15301 gaagcggcga ttaaagaact caattacgcg ccatcagctc tggcgcgtag cctcaaactc
    15361 aatcaaacac ataccattgg catgttgatc actgccagta ccaatccttt ctattcagaa
    15421 ctggtgcgtg gcgttgaacg cagctgcttc gaacgcggtt atagtctcgt cctttgcaat
    15481 accgaaggcg atgaacagcg gatgaatcgc aatctggaaa cgctgatgca aaaacgcgtt
    15541 gatggcttgc tgttactgtg caccgaaacg catcaacctt cgcgtgaaat catgcaacgt
    15601 tatccgacag tgcctactgt gatgatggac tgggctccgt tcgatggcga cagcgatctt
    15661 attcaggata actcgttgct gggcggagac ttagcaacgc aatatctgat cgataaaggt
    15721 catacccgta tcgcctgtat taccggcccg ctggataaaa ctccggcgcg cctgcggttg
    15781 gaaggttatc gggcggcgat gaaacgtgcg ggtctcaaca ttcctgatgg ctatgaagtc
    15841 actggtgatt ttgaatttaa cggcgggttt gacgctatgc gccaactgct atcacatccg
    15901 ctgcgtcctc aggccgtctt taccggaaat gacgctatgg ctgttggcgt ttaccaggcg
    15961 ttatatcagg cagagttaca ggttccgcag gatatcgcgg tgattggcta tgacgatatc
    16021 gaactggcaa gctttatgac gccaccatta accacgatcc atcaaccaaa agatgaactg
    16081 ggggagctgg cgattgatgt actcatccat cggataaccc agccgaccct tcagcaacaa
    16141 cgattacaac ttactccgat tctgatggaa cgtggttcgg cttagattta cgctgtcttt
    16201 tgatcaaatt attaccatcg gttgttttca gaagcatgaa cattgctgct gaagcaacag
    16261 taataatgcc cattgtgata aacgtatagt ggaattgttc gacagtcgtt gtgccttcca
    16321 ttccttcata aacgcgaagg acggccgcac ttacagctac gcctaaactg atagacagtt
    16381 gctgcgtgac cgccagaaca ctgttaccgc tgctggcgtt gtcatcggtc agatcggcaa
    16441 gtgtgatggt attcatcgcg gtaaactgcg tcgacatagc catccctaat ataaacaacg
    16501 gcaagatcag catccatatt gccattgccg gtgattgcaa agagaactga gcgatcatta
    16561 gcccaataat caccgtgatc cccactaatg tatggcgata gcccagacga cgtaagactt
    16621 gggtaaccat cgattttgca ataatggaac ctaacgctgt cggtgccatc atacagccag
    16681 caataaacgc ctgataacca aatcctacct gtaacattaa tggcataagg aacggtacac
    16741 agccggtccc cagacgggtt gcaatattgc ctacgatacc gatcgagaaa gtgcgggttt
    16801 taaataaatc taatgaaatt aatgggtttg gcgtgcgtcg tgcatggaga atatagagaa
    16861 gcagtaaccc gatgctggtg acaattaccg tcaaggcaat ccagctggcg acaatctttt
    16921 ccccgaatag ctctattccg cttgagaaga gaacaaggct gaggccaaac agcaaaaagc
    16981 cagtgatatc gaatctgcgt cgtgcggtgg tgaaattggg catatgtttg cgcgcgtaaa
    17041 gaaggcccgc aatacctatg gggatattga ttaaaaatat ccagtgccag gttgcccagg
    17101 tgaccagcac gccgccaaga atggggccta aaattggccc caccagaccc ggcatggcga
    17161 caaaattcaa tactggaaga agttcattac gaggataagc gcgcagtaag gccagccgag
    17221 caacaggcat catcattgcg ccgcctatcc cctgaataac ccggaagaca accagctgtg
    17281 gtagcgaatt agaaagtgcg caggccagag aacccaatgt gaacagactc acggcaaggg
    17341 taaaaatgcg acgcgtaccg aagcgatcgg ctagccatcc gcttaccgga ataagcatcg
    17401 ccaccgtcag cgtataactg atgatggctg attgcatcgc gagaggagaa cgattaaggc
    17461 tatgagcgat tgcgggtaag gcggtattca gaatagtggc atcaagtgcc tgcatgaaga
    17521 aggccatcgc cgcgatccac ggcaaacccg ccatactgcg cttcttttta tcgctcattc
    17581 aatgtcctgt tatcgggtta tcacttatca ggtgagcgta gcagcgcctg acaagcttta
    17641 aatgccgcgt cgccatcgct ttggataatc gcatcgacta tcgcctgatg cagatccagc
    17701 tttatcactg tgtcgctggt aattgacgtg aagtaagtgt gataaaccga atggaatagc
    17761 gaggcgaatg aggtcaaaaa cggattggcg ctcatttcat agatatgctc atgccaggcc
    17821 atatcgactt cgatccagcg ttcacggcga aagttctctt ttaatgccgc catttcggcc
    17881 attaacgtat tgagatgcgc tttctgttcc gcggtgccaa ccgttgctgc cagtaggcaa
    17941 gcttgtggct ccagacagat acgcataacc agaaagtgat cgatgacctg atgaaagttc
    18001 tcttctgtca tccaccaggt aagcaattcc tgatcaagaa aattccagtt cgattgtggc
    18061 atgacccggg taccaattcg cggtcgcggt aaaaccatcc cttttgccgt taacgttttg
    18121 accgcttcgc gtaccgctgt acgactcact ccaaattgct cgcccagctc aatctcaccg
    18181 ggcaaaatgg tgccgggttc atattcacct tttaagatcc gttgcgccag cttctcagcc
    18241 agaacatacg aaaggttttt ctgtgcagct aactgttgtg cgcttaaagg cattactaat
    18301 cttccttttt ctttttattc ctccttagta tgccaccagg aagtgtgatt acggttgcaa
    18361 aaacggcaaa ttgcttgttt tatggcacat taacggggct tttgctgaaa aaatgcgcgg
    18421 tcagaaaatt attttaaatt tcctcttgtc agaccggaat aactccctat aatgcgccac
    18481 cactgacacg gaacaacggc aagcacgccg ccgggtcagc ggggttctcc tgagaattcc
    18541 ggcagagaaa gcaaaaataa atgcttgact ctgtagcggg aaagcgtatt atgcacaccc
    18601 cgcgccgctg agaaaaagcg aagcggcact gctctttaac aatttatcag acaatctgtg
    18661 tgggcactcg aagatacgga ttcttaacgt cgcaagacga aaaatgaata ccaagtctca
    18721 agagtgaaca cgtaattcat tacgaagttt aattctttga gcatcaaact tttaaattga
    18781 agagtttgat catggctcag attgaacgct ggcggcaggc ctaacacatg caagtcgaac
    18841 ggtaacagga agaagcttgc ttctttgctg acgagtggcg gacgggtgag taatgtctgg
    18901 gaaact
//
LOCUS       DBFIFI010000074        18905 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.074, whole genome shotgun sequence.
ACCESSION   DBFIFI010000074 DBFIFI010000000
VERSION     DBFIFI010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.074"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(193..4095)
                     /gene="hrpA"
                     /locus_tag="N7B39_002499"
     CDS             complement(193..4095)
                     /gene="hrpA"
                     /locus_tag="N7B39_002499"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HGU2422463.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRIGHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWFNKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            4296..4901
                     /gene="azoR"
                     /locus_tag="N7B39_002500"
     CDS             4296..4901
                     /gene="azoR"
                     /locus_tag="N7B39_002500"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HGU2422464.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTTRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(4952..6244)
                     /gene="ynbD"
                     /locus_tag="N7B39_002501"
     CDS             complement(4952..6244)
                     /gene="ynbD"
                     /locus_tag="N7B39_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HGU2422465.1"
                     /translation="MLQGAGWLLLLAPFFFFTYGSLNQFTAVQDFNSHDIPSQVFGWE
                     TAIPFLPWTIVPYWSLDLLYGFSLFVCSSTFEQRRLVHRLILATVMACCGFFLYPLKF
                     SFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIVLCWLLWRHFRQHLAVRWRKVCGGW
                     FLLIAISMLTTWQHHFIDVITGLAVGMLIDWMIPVDRRWNYQKPDQRRIKIALPYVVG
                     ACACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQGKLPPAVYW
                     LTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTFEFPRGRAT
                     KDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSALVVAAWLL
                     CYGHCKTVDEAISFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(6258..8015)
                     /gene="ynbC"
                     /locus_tag="N7B39_002502"
     CDS             complement(6258..8015)
                     /gene="ynbC"
                     /locus_tag="N7B39_002502"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HGU2422466.1"
                     /translation="MENSRIPGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHIVDELAMPDTAFYAWDARGHGQTSGPRGYSPSLARSVRDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYHRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFISRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGT
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPGNEQVKNSLAGLAN
                     AIEPGGILLYTGQPWHPQLELIAGVLTSHKDGKPWVMRVRSQGEMDSLVRDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(8031..8927)
                     /gene="ynbB"
                     /locus_tag="N7B39_002503"
     CDS             complement(8031..8927)
                     /gene="ynbB"
                     /locus_tag="N7B39_002503"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HGU2422467.1"
                     /translation="MLEKSLATLFALLILATLINRFRVWRLPERKGDEVTLRIRTWWG
                     IVICFSLVISGPRWMTLTFFALISFLALKEYCTLISVHFPRWLYWVIPLNYLLIGFSC
                     FELFLLFIPLAGFLILTTWRVLVGDTSGFLHTVSAIFWGWIMTVFALSHAAWLLMLTT
                     TNTQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMI
                     ASLIIGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAVKRDVGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            complement(8927..9532)
                     /gene="ynbA"
                     /locus_tag="N7B39_002504"
     CDS             complement(8927..9532)
                     /gene="ynbA"
                     /locus_tag="N7B39_002504"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HGU2422468.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLAALALSLITGL
                     LLMLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGIRSYVGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLLMSAER"
     gene            complement(9703..12009)
                     /gene="ydbD"
                     /locus_tag="N7B39_002505"
     CDS             complement(9703..12009)
                     /gene="ydbD"
                     /locus_tag="N7B39_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HGU2422469.1"
                     /translation="MFKPRLCSWIGLLPLFMLSLPVQAELRCVANAVDIETFFSAATA
                     EDKQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLIVLGNLTVKGNIS
                     TFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNPSTIKGSINARGI
                     FINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWGLINDEDALKEIF
                     KRSNIRMAGNVCNQMKKEALFRLKPSPELVQELQMLDEGNVAAFEGRDIATFDLAIMR
                     TLPRLKGISANLRKQLINSNDGQTIESMARYMPDNEILELTDQQLGYQPVVLGLLDRE
                     PLSVEIMTRMSHLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPES
                     IIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNDVVTSLALKLAEMKHHGQLLRM
                     TPQESDKIAVYLYQKFENDDDLIGALFLALPDNLQFNFVKRMEKKSPAYFCCRDMQII
                     HSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKNNPKADSDAYETS
                     ADMILSELISYGEVDDQMLLNATSLIRSDDWDFLESALISWDNLPAVVLKELQQNTPR
                     NDIWAKFFLRQENSSRAQVNEALRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNLT
                     FFKFGALNNRHTPPAVLAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPLLALKLVNPE
                     LDLVRQLALNGKTRAIREQAMRKLDELY"
     gene            complement(12073..12933)
                     /gene="pdxI"
                     /locus_tag="N7B39_002506"
     CDS             complement(12073..12933)
                     /gene="pdxI"
                     /locus_tag="N7B39_002506"
                     /EC_number="1.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HGU2422470.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRVMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIEALARDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEKV
                     LSTLDGISRE"
     gene            complement(13153..15612)
                     /locus_tag="N7B39_002507"
     CDS             complement(13153..15612)
                     /locus_tag="N7B39_002507"
                     /inference="COORDINATES: protein motif:HMM:NF015737.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HGU2422471.1"
                     /translation="MNEGGALTNEGVLNTVTLEGGTFNNNGTLNDVVKIEKNSNAVIN
                     NTGSLSTLQLHDGTVNNSGIASARVNAQGDAVFNNLAGGEARKGAILYNSAVVNNAGT
                     WKMGYQDENNNAGTLDIDDKSTFNNSGKLILDNSKNAIRFQGSNANATLYNTGEMTLD
                     AALGAGAIHYDDGASEFINKGVVDAKVTVAVSTAGATESDAFLWNQDGGVINFDKDNA
                     SAVKFTHNNYVALNDGVMNISGNNAVAMEGDKNAQLVNNGVINLGTEGTTDTGLTGMQ
                     LDANATADAVIENNGTINIFANDSFAFSVLGTEGHIVNNGTVVIADGVTGSGLIKQGD
                     SVNVEGVNGNSGNNTEVHYTDYTLPDMPNTYTTSPFSETTDSGSSDGSSNNLNGYIVG
                     TNVDGSAGKLKVNNASMNGVGINTGFAAGTADTTVSFDNVVEGINLTDADAITSTSVV
                     WTAKGSTDASGNVDVIMSKNAYTDVATDASVNDVAKALDAGYTNNELYTSLNVGTTAE
                     LNSALKQVSGSQATTVFREARVLSNRFSMLADAAPKVGNGLAFNVVAKGDPRAELGNN
                     TEYDMLALRKTVDLSESQSMSLEYGIARLDGDGAQKAGDNGVTGGYSQFFGLKHQMSF
                     DNGMRWNNALRYDVHNLDSSRSVAYGDVSKTADTDVKQQYLELRSEGAKTFEPREGLK
                     ITPYAGVKLRHSLEGGYQERNAGDFNLSMNSGSETAVDSIVGLKLDYAGKGGWSANAT
                     LEGGPNLSYSKSQRTASLAGAGSQHFNVDDGQKGGGINSLASVGVKYSSKESSLNLDA
                     YHWKEDGISDKGVMLNFKKTF"
     gene            complement(15832..18867)
                     /locus_tag="N7B39_002508"
     CDS             complement(15832..18867)
                     /locus_tag="N7B39_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed virulence protein BigA"
                     /protein_id="HGU2422472.1"
                     /translation="MQRKKLLSVCVAMALSSQTWAADTSIPDSTEKTRKSSKITCPAN
                     IHSLSKEQLENLSAECRENKDSAVLPWAAAGLAAVATGIAIYTLSDDDNHHHNNSPVP
                     DDGGDTPVPPDDGGDTPVPDDGGDTPVPPDDGGDTPVPPDDGGDTPVPPDDGGDTPVP
                     PDDGGDTPVKHNPVVYKNGVTWDQDAKTVKIRETTFTYSQNADGSYTLTAPEGKETIV
                     KSWTVHDDSNTVVFDGVNTSGGITWSYDDDGLIHITKEAGVVVDGTTGNAIEFGNAII
                     TDQGGNTALNGGTVMTVDGDNISLNNDGKTTAIGEGSVVGILTGDNITINNNGETEVD
                     GGTAVIINGDNTKLNTAGDSTITNGGTGSLINGDNARVDNQGTMSVDGENSTGSKIVG
                     DGATIKQEGDLYVSGGAHGIDVDGNDTFVSNKGNITVIEDNSIGMLLDGDGVSVINMG
                     DLNVGQAAAGENAIGIQIDGDNATFVNVGDISATNAGTGVSVAGDKANISLAGGLDVG
                     DFSTGLDVSGNNNNMTLATYELNVTGQKATGVNVSGDGNTIEIAGSILVDKDQKADNA
                     QPYFFNPSTGVNISGDNNDVTLDGQLTVVADSKTTSRSYASYDGAQEHIAGIVIAGDD
                     NTFTLNGGVHFVGEKNVMDDGSKPSASRRGIGDTPLINVDGHSPVYLNGESTISGEFP
                     LGFENLIQLSHGAELEIGADATFDMSDVDSFTYYYRVALSTISIDSGAKATNNGEVEL
                     KNIGFAAAWNKDSTVINNGSIGLAMYDFGTDPAPKVFDVEYGGIGVNNGTMTTKMMNQ
                     HSVLNYPAEWNLSDGTSFNNKALGLTGMLASYSSSILNGETGIIDMYGRGSVGMLAID
                     KSTADNEGQITLDTLWVDENDETSLRNNVANSTAKDFGVGMASGTDAYNGALTKATAT
                     NHENGVITVYNAGAGMAAYGNTNTVINQGTINLEKNENYNDSLGVNKLVGMAVYHEGT
                     AINDQTGVININAENGQAFYNDGTGLIINYGTICTFGVC"
BASE COUNT         4750 a         5094 c         4279 g         4782 t
ORIGIN      
        1 attatccgtt caaattgcga cctcagtaaa atatcatgtc tactcattgc aatacgtcct
       61 gttcgcccaa ggcgaagtat gaatgaaact tatgtaaaat agtaccgccc ttgctatatt
      121 tcagaattca taaagaaaaa acccggcgct aaatgcttac cgggttttag atttatcagg
      181 caaatagcag ggttaaccgc taatctgctc cattgcctgc aaaatacgct tatctgaaat
      241 cggataaggc gtaccaagtt gttgagcgaa gtaactaacg cgcagctctt ctatcatcca
      301 acggatctct ttcacgtctt catcctcacg acgtgcaggc gggagtttgt tgaaccattg
      361 ctgccacgcc tgctggacgt tttcgacttt cagcatctgt gcacgatcgc gatgtggatc
      421 aaccgccagt ttttccagcc gtttttcaat cgcctgcaaa taacgcagcg tgtcgcccag
      481 ccgtttgaag ccgttaccag tgacaaaacc gcgatatacc aacccgccca tctgcgcttt
      541 aatgtcagaa agccccagcg ccatggtcat atccacccgc cctttcagac gtttgttgat
      601 attgaacact gccgtaagga tttgctcgac ctgcttcgca atatccacca ccgtgtcgtt
      661 cagttcggca cgcacttttt catgcagcgc agcaaagcct tcttccgtcc agaccgggcc
      721 tccattggcg tcgatcagtt gatccacacc gcatgagata cagtcgtcga tcagctccag
      781 cactttgcca tacgggttaa agtacagccc cagcttggct ttgtttggta acttctcatg
      841 taaatacttg attggtgagg gaatattcag cagcagtaga cggcgaagac cgttccacat
      901 tgcctgtttt tgctccagcg ggttatcaaa cagtttgatc gccacactgt cgcgctcatc
      961 caccagcgcc ggccacgcct tcactttgta gttaccacgc ttctgttcgt agctttccgg
     1021 caactgacca aaactccaga tatgtaagcc gctctgctcg ataccgtcat ccgccaccgc
     1081 agatagcgtt tcctgcactt tgcctttcag cgcatctttc agatcttgta gcgagcgccc
     1141 ttcttttagc ttcttgtttt tgtcatccac cacgcggaag gtgattttca ggtgatcggg
     1201 cacctgatcc cagtgccagt cttcgcggtc aacggtaacg ccggtcatcc gccgtaactc
     1261 gcgctcaagg ctgtcgagca acggtaactc cagcggtgtg acgcggccta aaaacgcttc
     1321 ggcatagttt ggcgcaggta caaaattacg gcgtaccggt ttcggcaacg atttaatcag
     1381 agcaatcacc agttcgcggc gcagaccagg gatctgccat tcaaacccgc tttcctcaac
     1441 ctggttaagt aacggcagcg gaatatgtac ggtcacaccg tcagcatccg cgccgggctc
     1501 aaactgatag ctcaaacgca gcttgagatt gccctgatgc cagaagttcg ggtaatccag
     1561 cttgctgatc ttttccgccc cttctttgat caacatgctc ttttcaaagt tgagcaaatc
     1621 aggcgtttcg cggctgactt ttttccacca gctgtcgaag tgacgagcgg agattacatc
     1681 gtggccgatg cgctggtcgt agaactcaaa cagcgtttcg tcatcaacca gaatatcgcg
     1741 gcgacgtgat ttgtgttcaa gctcttccac ttcggcccgc agtttcaggt tttcacggaa
     1801 gaatgcgtga cgcgtctgcc agtcaccttc caccagcgcg tggcgaataa agagttcacg
     1861 acataacgcc ggatcgatct ggctgtagtt gaccttgcgc gcggcaacaa tcggcaaacc
     1921 ataaacagtg actttttccg ttgccatcac cgcgccctgc gcccgttccc agtgcggttc
     1981 gctgtaggta cgtttaatca aatgctgagc gacaggctcc acccattccg ggtcgatacg
     2041 cgcagcaatg cgcccccaca ggcggctggt ttctaccagt tccgccacca ttacccattt
     2101 cggcggtttt ttgaataaac cagaaccggg gaagatggag aaacgcgcgt tacgtgcgcc
     2161 ggtatattct tgtttatcgg catctttcat gccgatatgg gaaagcaacc ccgtcagcaa
     2221 cgcaatgtga atttcacgat actccgccgg ttcgctgttc accggaatgc cgagttcttt
     2281 caccacctgg cgcaactggg tgtagatatc ctgccattcg cgcacacgca gatagttgag
     2341 ataatcggta cgacacaggc gacggaaggc gttggaagaa agcgcctttt gctgctcgcc
     2401 aagataattc cacagattca caaacgcgag gaagtcagac tctttgtcgt ggaagcgacg
     2461 atgtttttcg tccgatgcct gctgtttgtc catcggacgt tcgcgcggat cctgaatgga
     2521 gagcgcagac gtgataatca tcgcctcacg cacgcagcca tgtttttgcg cttccagcac
     2581 catacgcgcc agacgtgggt cgacaggcaa ctgcgagagc tggcgaccga gcggcgtcag
     2641 tttataggcg ctggcctgtt catccgtggt gatcgcgccc agttcttcga gcagacgcac
     2701 gccatcctgg atattgcgtt tatccggtgc ttcgacaaac gggaacgcag cgatatcgcc
     2761 tagccccagc gcggtcatct gcaaaataac cgaggccagg ttggtacgca gaatctccgg
     2821 atcggtaaac tccgggcgcg agaggaaatc gtcttcggaa tagagacgaa tacagatccc
     2881 ttcggacaca cgaccacagc ggcctttacg ctgattggca gacgcctgcg aaatgggctc
     2941 aatcggcaaa cgctgcactt tggtgcgata gctgtagcgg ctgatacgcg ccgtaccggg
     3001 gtcgataacg tatttaatcc ccggtacagt cagcgaggtt tccgcgacgt tggtcgccag
     3061 cacaatgcgt cgcccgctgt gcgactggaa cacgcggttc tgttcgctat tcgaaagccg
     3121 cgcataaagc ggcaagattt cggtatggcg taagttcagc ttgttcagcg catcggcggt
     3181 atcgcgaatt tcccgctcgc cgctcataaa gatcagaatg tcgccggggc tttcctggct
     3241 tagttcgtcc acggcatcga aaattgcctg caactggtcg cgctcagtgt cgtcggcttc
     3301 ttcaacaatc gggcgatagc gcacttccac cggataggtc cgaccggaga cttcaataat
     3361 cggcgcatta ttaaagtggc gcgaaaagcg ttccgggtcg atagtcgcgg aagtgataat
     3421 gatttttagg tcaggacgcc gcggcagcaa ctctttcaaa taaccgagca gaaaatcgat
     3481 attcaggcta cgttcgtgcg cttcgtcaat aatgatagtg tcgtactgca tcagcaggcg
     3541 gtcttgctgg atctccgcca gcaggatacc gtcggtcatt agcttgacca tcgtgttatc
     3601 acttacgtga tcgctgaaac gcactttgta accgatgcaa ccgcccggct ccgttttcag
     3661 ctcttccgca atacggttcg ctaccgttct tgccgccagg cgacgcggct gggtatggcc
     3721 gatcagccct ttaatcccgc gccccagctc catacagatt ttcggtaact gagtcgtttt
     3781 accagaaccc gtttccccgg cgactatcac cacctggtga tcacgaatcg cttcgagaat
     3841 gtcctgtttt ttctgactaa ccggcaaatt gtcaggataa gtaatttccg gtcgtgccgc
     3901 ttcacgcagc aggactttcc ctgccgcctg gtcaatctct ttcgccatct cctggaaaat
     3961 ggcctgttgt gcatcaggat ttttaacctt cttcacgccg tgcagacggc gagaaaaacg
     4021 cagtctgtca cgcagcatca gcgaatccag ccgctgctgc aaggccgtaa aagtcaattt
     4081 ttgttgttct gtcataacgt tagtgggcag tagtctgcct gattaatttt ctttgaaatg
     4141 atagatatag agtaccacat cgcggctttc tatgcgttgt tcaataaatt cgaacatagg
     4201 cttcgattta ttgcgctatc tctgtggcag gattgtgaat aaagtgtcaa caagcaacgg
     4261 ggcatcgccc attcaaatat ctataaggaa acaccatgag caaggtatta gttcttaaat
     4321 ccagcatcct ggcagggtac tctcagtcta atcagttgtc cgattatttt gttgaacaat
     4381 ggcgcgaaaa gcactccgct gatgaaatca ccgttcgcga cctggctgca aatccaattc
     4441 cggtactgga tggcgaactg gttggcgctc tgcgtccgag cgatgcgccg ctgactacgc
     4501 gtcagcagga agctctggca ctttccgatg agctgattgc cgagctgaaa gcccatgacg
     4561 ttatcgttat tgcggcaccg atgtataact tcaacatctc gactcagctg aaaaactatt
     4621 ttgacctggt tgcacgtgca ggcgttactt tccgctatac cgagaatggt ccggaaggtc
     4681 tggtaacggg taaaaaagcc atcgttatta ccagccgcgg tgggatccac aaagatggac
     4741 caacggacct ggtgacgccg tatctgtcca cgttcctggg ctttatcggc attaccgatg
     4801 tgaaatttgt cttcgccgaa gggatcgcat acggtccgga aatggcagcg aaagcgcagt
     4861 ctgacgcgaa agcagccatc gacagtattg ttgctgcata accggacacg atcttgatgt
     4921 ggactgtagt tcagtccaca tctcaatcca cttacctgtt ttcccataat ttcaacatcg
     4981 ctttgtgctc ttccttaagc acaatatgcg agcgtctggc tcgaataaag ctaatcgctt
     5041 catcaactgt tttacagtgt ccgtaacata acagccatgc cgccactacc agcgcactgc
     5101 gcgataatcc caatgcgcaa tggaccagaa cgctgccttg ctcttcgcgt aatgtttcca
     5161 gcatcgccac ggcctgtcgg agctccccct cttccggaac caccagatcc agcatcggta
     5221 cacagaaata gagtcgatct tttgtcgctc gtccgcgagg gaattcaaag gtgacatcca
     5281 gaaccgcatt ctgtgccgga atatgtcgtg gaaacgcccc taaataaaca ccagcagtca
     5341 ttttgctcac cggctccagg cgacgacaaa accaacgcat agatagccac atcccgatgc
     5401 gccagggcaa tgtcagccag taaacggcgg gcgggagttt cccctgacta tctttccctg
     5461 ttgttatcgc gccaagccca ccgtacccac ggccaataat gagtagcgat aatactggcc
     5521 aacataacca gactgaccac cataactgaa ccatcatcat tagctccatc aacacaatgc
     5581 acgcgcacgc gcctacgaca tatggcagtg ctattttgat tcgacgttga tcaggtttct
     5641 gataattcca acgacggtcg acgggtatca tccagtcaat caacatacct accgccagcc
     5701 ctgtgatgac atcaataaaa tgatgctgcc aggttgtcag catcgaaatg gcgatgagta
     5761 aaaaccatcc gccgcagact ttacgccacc tcacagccag atgctgacga aagtgacgcc
     5821 aaagtagcca gcagagaaca atatgcagcg aaggagactg gttataaggc agatcaaaca
     5881 gttcaagttg cgaaaatagc catcctgtca ccccactcac ttcaggacgg ataaaactaa
     5941 acttcagcgg gtagagaaaa aaaccgcagc aggccattac cgttgccaga ataagccggt
     6001 ggacaagtcg gcgctgttcg aatgtcgagc tacaaacgaa cagcgaaaat ccatataaaa
     6061 gatccagact ccagtaaggg acaatagtcc agggaagaaa agggatcgcc gtttcccagc
     6121 cgaatacctg actggggata tcatggctgt taaagtcctg aaccgcggtg aactgattta
     6181 gagatccata ggtgaagaag aaaaacgggg ccagcaacaa taaccagcca gcgccttgta
     6241 gcaatacgtt ccggcgttca gttatcacga cgcaccgcca tcgaaaccgt aaaaatgccc
     6301 cactcatcaa tccgttgtgt gcatttatca aatccggcat cacgcacgag tgaatccatc
     6361 tccccttgcg aacgcacgcg cattacccac ggtttaccat ctttatgact ggttaacacc
     6421 ccggcaatca gctccagttg tgggtgccac ggctgcccgg tgtagaggag aatgccgccc
     6481 ggttcgatgg cattggcaag acctgcgagt gagtttttta cctgctcatt tccgggaaaa
     6541 agctcataca gtccagagac aatcgccagc gtaggccgcg gagttaacgc gctgagttcc
     6601 tccgggttaa acgcatcgcc ctgttcaaaa cgcacccgcc cagacattcc ccgttgagca
     6661 atcatctctt gcccctgtgc aacatttaac tcgctgtaat cacgtaacaa aatatcgctt
     6721 acggcaggct cgtttgccag cgcatccagt acatagcgcc catgccctgc ggcaatgtca
     6781 accacgcgga cggctaaacc tttggcgtgg agatcggcaa cggcttgttt aatcagtatt
     6841 tgtaaatggg ttttgcgctg gcgaataccg cgccagccaa cactgttcag gtagattttg
     6901 tcgattaagc gcccgaatgc gttactacct tgcggttgat tttgatagac ataatccagc
     6961 gaactgcctg aatcaaagcc ggtgctcatt ccgagatgca ggcccgcaga gtgcgtcccg
     7021 aacaatttca tcgctttacg cataaagcga tacgccaaat caaccggcga taatggcacg
     7081 ggtccacctg aaaggagccg ccagcgatcc gctgatggtc cagtgcggtc ttcatgctga
     7141 taatcaaatt tttgtgactt gttagcatat aaacgactaa taaagctttg cattttttca
     7201 aatgcctgcg ccctgttctc ttcacccaac gtgtcgtgat aaaagcctgg cagcagatgc
     7261 agctctttca gagggctacg taatctatga taaaaatcaa tctgcggttg gcgatgcacc
     7321 acatagtcat cgcctgatat cagaagttgc gtggggagcg taatcgccgc cgcatcgcta
     7381 acaatacgtt cagacgtttt ataaagatcg agcaagatat taacggcaat cgcccgtgtg
     7441 atcagcggat cattattgaa actcgccacc cgttgccgat cgtgggtcaa atagcgtcct
     7501 ttcacatagg aattaataaa aaacagacca cgcagacgat gccataacgc cagcgcagga
     7561 cgtgccagcg gcacatacaa tttaacctta aaggccggag aagccagcac cagcccgcga
     7621 attgcaggcg cataatcatg aacccatgtg gcaaccagca ctgcgccgac gctctgcgcg
     7681 atcactacca cctcttccag cccgacctgg ctgtcgctgg cagcaaaacg gacaaactca
     7741 tcgacatccc gcactgaacg cgcaagagat gggctataac cacggggccc cgaagtttgt
     7801 ccatgccctc gggcatccca tgcataaaaa gcagtatctg gcatcgccag ttcatcaacg
     7861 atatgttgta gacgaccaga atgttcatga ccgcgatgaa ataagacgat gacctttttc
     7921 gcccctggct gtaaagcggg ccagtgccga taaaacaacg ctgtattatc actggtggta
     7981 aaaaaatgtt ccccagggat gcgtgaattt tccattttct ctattttcct tcagtaacag
     8041 cagtagcgaa taaaataaaa aaataccgga gcggtgaaaa ttaatgagtc aatcctgtca
     8101 aggagtccgc catgtcctgg taatagtttt ccgctatctt taacacccac atctcgtttg
     8161 acggctgaca tcactacatc gccgcaaaaa ccactaatac caattaacaa acccgctaat
     8221 aatgcctgta atgtatttag cggtgtcagc aacggtccaa taatcagtga cgcaatcatg
     8281 gtggtgatta cgccccccac cagaccttcc agcgtttttc ccgggctgac tttggggacc
     8341 acttttcttc tgccgcagga tttcccccat aaatactgtg caatatcgtt tgactctgtt
     8401 aacgccaaaa gaaataacac cagtaacgcc ccgccctggg tatttgtggt tgtcaacatt
     8461 aataaccagg cggcatgact caaggcgaaa acggtcatta tccagcccca aaaaatggcg
     8521 ctcacggtat gcagaaaacc ggaggtatca cctactaaaa ctcgccaggt ggttaatatc
     8581 agaaacccag ccaaaggtat aaataacaga aacagctcaa agcaactaaa accgataagc
     8641 aaatagttaa gaggaataac ccaatataac caacgcggaa aatgtacgga tataagcgta
     8701 caatattctt tcaatgccag aaaacttatc agcgcaaaaa acgtcaacgt catccagcgt
     8761 ggacctgaaa tcaccagtga aaaacaaatg acgatacccc accaggtacg aatacgtagt
     8821 gtaacttcat ccccttttct ctccggtaac cgccataccc gaaaacggtt tatcagcgtt
     8881 gctaaaatta acaacgcgaa aagtgttgcc agagattttt ccagcattat ctttcagcac
     8941 tcatcaggag cacactccga caacgattaa tcgcagtcca gagaagcaga attgaagcaa
     9001 tgctccataa aagattattc cattgcatcc actgtggata aatggcaacg gccagccccc
     9061 acaacccaaa tattaacgcg cgatcgcttt tgccaaaagg accgacataa ctacgaatac
     9121 cgttaatcgt ctgcgcgagt aaaccgcaaa actcagtcaa tatggtgcaa aataacatga
     9181 gtattacgag tgatgcgtta ctttccggta aaaataaaaa cggtaaatag agcgcaatat
     9241 cggaaataac atcgccagtt tcatttaaaa tcgcccccag acgtgtttgc tgattgcact
     9301 cacgcgccaa catgccatcc agcgcattga gtgccatacg gataaaaagc acgatgggca
     9361 atagcaaaaa gaggatgggt tgtgcgacca acatcaacag caatccggta ataagagaaa
     9421 gcgccagtgc agcaagagtg atgtgattcg ctgtaacatg gtgcttataa agccaaaaca
     9481 tcgtcggcct taacagcgac tgaaagagcg gttttatctg gtagagcgtc attatcaatc
     9541 cttgataatt cggtttgcgc tatggtgcat caattcccct gacggagata tcgcaacacg
     9601 acgcttcatt aaaaattcag aatatatcaa ggtattgggg actcactgtc aggaatgatt
     9661 atccccaata aatcaagtgg ttaaaaatta ttacgctgtt attcaataca actcatcaag
     9721 ttttctcatc gcttgttccc gtattgcgcg cgtcttaccg ttaagcgcca gctggcgaac
     9781 taaatccagt tcagggttaa caagctttaa cgccagcaaa ggatcacgtt tcagcctcgc
     9841 ctttaataca tcaactggaa aacggggatt attcatcgcc acaatccacc actcgggatc
     9901 aatttctgcc gccagtactg caggcggtgt gtggcggttg ttaagtgcgc cgaacttgaa
     9961 aaatgtgaga ttgcttttcg ccagtgtact ccaccatata cgatccggtt gttttgccag
    10021 tacatccagt tgtgctaacg catcggggtc tagtgcataa taaacacgta acgcttcgtt
    10081 aacctgcgca cgggaactgt tttcctgcct cagaaaaaac ttcgcccaaa tatcattgcg
    10141 tggcgtgttt tgctgtaatt ccttgagaac aacagcgggt aaattatccc aactaattaa
    10201 tgcactctct aaaaagtccc agtcgtccga acgtatcaga gaggtggcat ttaatagcat
    10261 ctggtcatca acttcaccgt agctgataag ctcagacaaa atcatatcag cgctggtttc
    10321 atatgcatct gaatctgctt tgggattatt tttccgatgc gataaagcac gttgccaaat
    10381 tttttgtttc atcgacgtgc tcagatactg atttttcgcc agattactcc acccctcagg
    10441 atcgttgaaa cgtgtcaata accgttgtaa agcagcgtca gagtgaatta tctgcatatc
    10501 ccgacagcaa aagtaggcag gggatttttt ctccatcctt ttaacaaaat taaactgtaa
    10561 gttatctggc aacgctaaaa ataacgcacc tattagatca tcgtcattct cgaatttttg
    10621 gtataaatat actgctattt tgtcactctc ttgtggcgtc atccgcaaca attgaccatg
    10681 gtgtttcatc tctgccagct ttaacgctaa gcttgtgaca acatcgtttg atgaatcgtt
    10741 cgccaactgc attgcctgat tgtaggtgag ttgtcctcca gcaccgacct gacgaataga
    10801 ggaatcatca ctgcggatat agccatcaat aatggattcg ggcaataacc acgcatcttt
    10861 ataaagctcc tgaatgatca tatcccaatc tcttttcgcc agagtcatga cgatatccag
    10921 agggagattt tcacgtagcg caagattcaa cggaccgaca ccatcaggta gatgactcat
    10981 tcgcgtcatt atttcgacag agagcggttc acggtcgagc aaccccagaa caacaggctg
    11041 gtaacccaat tgctgatcgg tcaactccag aatctcattg tcaggcatat atctcgccat
    11101 actttcaatc gtttgtccat cattgctatt aataagttgt ttgcgtaggt tagctgaaat
    11161 tcctttaagc cttgggagag ttcgcattat ggcaagatca aatgtcgcaa tatctcgtcc
    11221 ttcaaatgca gcaacgttgc cttcatccag catttgcaat tcctgcacta actcaggaga
    11281 aggcttaagg cgaaacagcg cttctttttt catctgatta cagacattcc ctgccatgcg
    11341 aatattgctt cgcttaaaaa tttctttcag agcatcttca tcatttatta gaccccaata
    11401 atatgcatca ggatttatta tcatcagcgc ttttttgaca ttttcagtgt catttttgtc
    11461 actcgcacgg accatgaatt cactatttgt cgaagacgca acaacaggtg caattatgtc
    11521 attgataaat atcccacgcg cattaatact ccccttaatc gtagacggat tatcgtaata
    11581 tgagtcgata aataccagtc cacttgcatt tatcgaaccg gtaattaaca gtggtgaatc
    11641 cgtaactata ttcgttgccg tcacgttacc gagaataacc catggattag aaagagagaa
    11701 agtggaaata ttacctttga ctgtcaaatt gccaagaaca atcaattttt gattactctc
    11761 tatattacct tctaccacta aatcgccacg atgcactttc cagtccgatg cagataaacc
    11821 gaaggggaca agattcacgc tactgttgat agcttgttca acttgttgtt tatcttcagc
    11881 ggtagccgca gaaaaaaaag tttcaatatc aactgcattt gcaacgcatc gaagttccgc
    11941 ctgtactgga agactcagca taaacagggg aagtaaacct atccagctgc aaagcctggg
    12001 cttaaacata tgcactccat gccattaaaa aagaaacaaa agggaaatgc atctttccct
    12061 tttgtatatt cgttattctc gcgaaatacc atccaacgta gacaacactt tctcagaaag
    12121 atgcaacttt tcagcagcca tattctcccg taaatgcgca acagaagacg tccctgggat
    12181 cagcaaaata ttcggtgaac gttgtaacag ccacgccagc gccacctgca ttggtgttgc
    12241 acccaagctc gcagcaacat cggaaagcgt ggaagattgc agcggcgtaa agcccccgag
    12301 cggaaagaac ggcacgtagg caatgccgtc acgagccaaa gcttcaatca ttgcgtcatc
    12361 agcacgatga gcaatgttgt attcgttctg cacacagacg atttcggcaa tcttgcgcgc
    12421 ctctgcaacc tgcgtcggtg tgacgttgct taggccaata tgttttacca ggccttgttg
    12481 ttgcatctct gccagcacgg tcaggctggc ttcaatcgat ccttccgccg ggccatgacc
    12541 atcccccatc atcacgcgca ggttaaccac atccagtacg tccaggccga gattacgtag
    12601 attatcgtgc accgcttttt gcagctctgc cggagaaaat gcgggcaacc aggatgcgtc
    12661 ctctccacgc cgcgcaccaa ttttagtgac aattgtcaga tcgtcagagt aaggataaag
    12721 cgcttcgcgg ataatctgat tggtgacgtg cggaccataa aagtcgctgg tatcaatgtg
    12781 attgacgccc aatgccagcg cctcacgcag cacggttata gcgacgtgac gatctcttgg
    12841 ggggccaaaa accccaggac ctgccagttg catcgcgcca taaccaagac ggttaacgga
    12901 ttttgtaccg agagtaaatg tattgctgct catcgcaccc tgccctgtaa taaaacggat
    12961 taagaaaaat cagtatagaa acgaaaagag ataaataatg cgaaggcgta aacaagattt
    13021 ctggatgagt agcactattg tcctgaaaga caccaaaaca ttagacagca aaagataagt
    13081 caagcgtcgc atccggcaat aaaaaaccgc aacctgtatc agcgttgcgg tttgtgtatc
    13141 acatgctaaa aattagaacg ttttcttaaa gttcagcatc acgcctttgt cgctgatgcc
    13201 gtcctctttc cagtgatacg catccagatt cagcgaactt tctttgctac tgtacttcac
    13261 gccgacgctc gccaggctgt tgataccgcc gcccttctga ccgtcatcga cgttaaagtg
    13321 ctggctgcct gcccctgcaa ggcttgccgt gcgctggctc ttgctgtagc tcaggttcgg
    13381 cccgccttcc agcgtcgcat tcgcgctcca gccgcctttc cctgcgtagt ccagtttcag
    13441 cccgacgatg ctgtccaccg ccgtttcgct gccgctgttc atgctcaggt taaagtctcc
    13501 ggcattgcgc tcctgatagc cgccttccag cgagtgacgc agtttcactc cggcgtacgg
    13561 ggtgattttc agcccttcgc gcggctcaaa ggttttcgcc ccttcgctac gcaactccag
    13621 gtactgctgt ttcacatccg tatccgccgt tttgctgacg tcgccgtaag cgaccgagcg
    13681 gctgctgtcg agattatgca cgtcataacg cagcgcgttg ttccaacgca taccattgtc
    13741 gaaggacatc tggtgcttca ggccaaagaa ctggctgtag ccgccggtta cgccattgtc
    13801 gcccgctttc tgcgcaccgt cgccatccag acgcgcgata ccgtattcca ggctcatgct
    13861 ctggctttcg ctcaggtcaa cggttttacg cagtgccagc atgtcatact cggtgttatt
    13921 tccgagttcc gcacgcgggt cacctttcgc caccacgtta aaggccaggc cattgcccac
    13981 cttcggcgcg gcatccgcca gcatgctgaa gcggttgctt aacacacgcg cttcacggaa
    14041 taccgtggtc gcctggctac cgctcacctg cttcagggcg ctattcagtt cagcagtggt
    14101 gcccacgttc aggctggtat acagctcgtt attggtgtaa cccgcatcca gtgccttcgc
    14161 cacatcgttc accgaagcat cggtcgccac atcggtgtag gcgtttttgc tcatgatgac
    14221 gtcaacgttg ccgctggcat cggtgctgcc tttggcggtc cataccacgg acgttgaggt
    14281 gatggcatcg gcgtcggtca ggttgatgcc ttccaccacg ttgtcgaaac tgaccgtggt
    14341 gtctgccgta ccggcggcga aaccggtgtt gatcccgaca ccattcatgc tggcgttgtt
    14401 gaccttcagt ttaccggcgc tgccgtcaac gttggtaccg acgatatagc cgttgaggtt
    14461 gttactgctg ccatcgctac taccgctatc agtcgtttca ctgaaaggtg aagtggtgta
    14521 agtgtttggc atgtccggca acgtgtagtc ggtgtaatgc acttcggtat tgttaccgct
    14581 gttgccgttc accccttcca cattcacgct gtcgccctgc ttaatcaacc ccgaaccagt
    14641 cacgccgtcg gcaatcacca ccgtaccgtt gttaacgata tgaccttctg tgcccagtac
    14701 gctgaacgca aacgagtcgt tagcaaaaat attgatcgtg ccgttgttct caattaccgc
    14761 atcggcggtg gcattggcat ccagttgcat accagtcaat ccggtatcgg tggtgccttc
    14821 ggtaccgaga ttgataacgc cgttgttaac cagctgtgcg tttttatcgc cttccatcgc
    14881 cacggcgttg ttgccgctga tgttcattac accatcgttg agagcaacat agttgttgtg
    14941 ggtgaattta accgcgctgg cgttgtcttt atcgaagtta attaccccgc catcctggtt
    15001 ccacaagaag gcatcgcttt ctgtcgcacc ggcagtactt accgccacgg tgactttcgc
    15061 atccaccacg cccttgttaa taaactcgct ggcgccatcg tcgtagtgaa tagcgcccgc
    15121 acctaatgcg gcatccagcg tcatttcacc ggtgttatat aacgtggcgt tagcattgct
    15181 gccctggaag cgaatggcgt ttttgctgtt atcaaggatg agtttgccgc tgttgttgaa
    15241 cgttgactta tcgtcaatat ccagcgtccc ggcattgttg ttttcatcct gataacccat
    15301 tttccaggta cccgcgttat tcactaccgc agagttatag agaatcgcgc ctttacgcgc
    15361 ctcgccgcct gcaaggttat tgaataccgc atcgccctga gcgttaacgc gcgccgacgc
    15421 aataccgctg ttattcaccg taccatcgtg aagttgtaaa gttgacaggg aaccggtgtt
    15481 attaattacc gcgttgctgt ttttctcaat ttttacgacg tcattaagcg ttccgttgtt
    15541 attgaaggtt cctccttcca gagtcaccgt gttcaataca ccttcattgg ttaacgcacc
    15601 gccttcattc acgacgatgg ctgagctaat ggttccgctc gactcgttaa ccagcgcacc
    15661 attttcgagt atcctgatcg ttccattttt aacagcaccc gcactggtga catccccgtt
    15721 gataatcgca cctgacgata ataatacggt ctcacccgca gaacttaata cagcgccatc
    15781 atcatataaa tcagcaatat aattatccgt tggattatat tcatcggccc cctagcaaac
    15841 gccaaaggta caaattgtgc catagttgat aataagccca gtgccatcgt tatagaatgc
    15901 ctgaccattt tccgcgttaa tgttgattac gcctgtctgg tcattaatgg cggtgccttc
    15961 gtgataaacc gccatgccga cgagtttatt cacaccgaga gaatcgttgt aattttcatt
    16021 tttctccagg ttgatggttc cctggttaat gaccgtatta gtattcccat acgccgccat
    16081 ccccgcaccg gcgttgtaga cagtaatcac accattctcg tggttggttg ccgtggcttt
    16141 agttaatgcc ccattatagg catcggtacc tgaagccatt cccacaccga aatctttcgc
    16201 cgtgctattt gcaacattat tacgcagcga tgtttcatca ttttcatcaa cccacagcgt
    16261 atccagggtg atttgccctt cgttatccgc ggttgattta tcgatggcta gcatccctac
    16321 actgccacgg ccatacatat caataatacc cgtttcgccg ttcagaatgg atgacgagta
    16381 gctggcaagc atacctgtca gccctaatgc cttgttattg aaagaggtac catcagacaa
    16441 gttccactct gccggataat tcagcacact gtgctgattc atcattttgg tggtcatggt
    16501 accattgtta acgccaatcc caccgtattc aacatcaaag acttttggtg ccgggtcggt
    16561 accaaaatcg tacatcgcca ggccaatgga tccgttattg attaccgttg aatctttatt
    16621 ccaggcagcc gcaaaaccga tatttttcag ttccacttca ccgttgtttg tcgccttcgc
    16681 accagaatca atactaatgg tggataacgc tacacgataa taataagtga aagaatccac
    16741 atcactcata tcaaacgttg cgtcagcgcc aatctccagc tccgcgccat ggctcaactg
    16801 aatgagattt tcgaacccca gcgggaactc gccgctgatg gttgactcac cattcaggta
    16861 gaccggggaa tgcccatcaa cgttaataag cggtgtgtct ccgataccac gtcgagaagc
    16921 tgatggtttt gagccatcat ccatgacatt tttttcaccg acaaaatgga caccgccatt
    16981 aagcgtaaag gtattgtcat cgccagcaat aacaatacct gcaatgtgtt cctgagcacc
    17041 gtcgtaactc gcataggaac gcgacgtggt tttactatca gcaacaacgg ttaactgacc
    17101 atccagcgtg acgtcgttgt tgtcaccgct gatatttacc ccggttgagg gattaaagaa
    17161 gtaaggctgc gcattatcgg ctttctgatc tttatcaacc agaatactgc ccgctatttc
    17221 gatagtatta ccatcacccg aaacattaac acccgtcgct ttttgccctg tcacattcag
    17281 ctcataagtc gccagcgtca tgttgttatt attgccagag acatcaagac cggtggagaa
    17341 atccccaacg tctaacccac ccgcgagtga aatattagct ttatccccag caacgctgac
    17401 accggttccg gcattggtcg cggaaatatc accaacgtta acaaatgtcg cattatcacc
    17461 gtcgatctga atgccaatgg cattttcacc cgctgcggcc tgaccaacat tgaggtcgcc
    17521 catgttgatc acgctgacgc catcaccatc aagcagcatg ccaatagagt tatcttcgat
    17581 gaccgtaata ttacctttgt tgctaacgaa cgtatcattg ccgtcaacgt caataccgtg
    17641 cgcaccaccg cttacgtata agtcgccttc ctgtttgatg gtggcaccgt caccgacaat
    17701 tttcgagcca gtggaattct ctccgtcaac gctcatggta ccttgattat cgacccgcgc
    17761 attatcgcca ttgataaggc tacccgtacc accattggtg atggtcgagt cgcccgcagt
    17821 gtttagcttg gtattatcgc cattgatgat taccgcggta ccgccatcaa cttccgtctc
    17881 gccattattg ttaatggtga tattgtcgcc agtgagtata ccgacaaccg agccttctcc
    17941 gatggccgtc gttttgccgt cgttgttgag ggagatgttg tcaccatcaa ccgtcatgac
    18001 agtaccgcca ttcagcgccg tgttgccgcc ctgatcggtg atgatcgcgt taccaaactc
    18061 aatcgcattc ccggttgtgc cgtcaacaac gacgccggcc tctttagtga tgtggatgag
    18121 tccatcatcg tcgtaactcc aggtgatgcc acctgaggta tttacgccat caaagacaac
    18181 ggtgttgctg tcgtcatgga ctgtccagga cttaactatc gtctccttgc cctcaggcgc
    18241 cgttaaggtg tagctcccat cggcattttg gctgtaggta aaggtagttt cgcgaatttt
    18301 gacagttttt gcatcctgat cccaagttac gccattttta tagacgacag gattgtgttt
    18361 aaccggggta tcgccaccat cgtctggcgg aactggcgta tcaccgccgt catctggcgg
    18421 aaccggcgta tcaccgccgt catcaggcgg aactggcgta tcaccgccgt cgtctggcgg
    18481 aacaggcgta tcaccgccat catccggaac tggcgtatca ccgccgtcgt ctggcggaac
    18541 aggcgtatca ccgccatcat ccggaactgg ggagttatta tggtggtgat tgtcatcgtc
    18601 acttaaggta tatatagcaa ttcccgtagc tactgcagca agacctgcag ctgcccatgg
    18661 taaaactgca ctatctttat tttctctgca ctccgcggat aaattttcta gctgctcttt
    18721 gctcagggaa tgaatatttg ccggacatgt tatcttactg gacttacgtg ttttttcggt
    18781 tgagtcgggt attgaagtat ccgcagccca tgtctggcta cttaacgcca tggcaacaca
    18841 aacagacaat aattttttcc tttgcatata aaattcctta gatctagacc gtgcgtaaca
    18901 agcca
//
LOCUS       DBFIFI010000075        18590 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.075, whole genome shotgun sequence.
ACCESSION   DBFIFI010000075 DBFIFI010000000
VERSION     DBFIFI010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.075"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(85..348)
                     /gene="cedA"
                     /locus_tag="N7B39_002509"
     CDS             complement(85..348)
                     /gene="cedA"
                     /locus_tag="N7B39_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HGU2422473.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            531..2792
                     /gene="katE"
                     /locus_tag="N7B39_002510"
     CDS             531..2792
                     /gene="katE"
                     /locus_tag="N7B39_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HGU2422474.1"
                     /translation="MSQQNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTASGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGSRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(2839..3597)
                     /gene="chbG"
                     /locus_tag="N7B39_002511"
     CDS             complement(2839..3597)
                     /gene="chbG"
                     /locus_tag="N7B39_002511"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HGU2422475.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMNFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSHGFSSAFYGEEISEALFLQVLDDSSHRGERSLEVMCHPAFVDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(3610..4962)
                     /gene="celF"
                     /locus_tag="N7B39_002512"
                     /gene_synonym="chbF"
     CDS             complement(3610..4962)
                     /gene="celF"
                     /locus_tag="N7B39_002512"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HGU2422476.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCTLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNGVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5067..5909)
                     /gene="chbR"
                     /locus_tag="N7B39_002513"
     CDS             complement(5067..5909)
                     /gene="chbR"
                     /locus_tag="N7B39_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HGU2422477.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(5917..6267)
                     /gene="chbA"
                     /locus_tag="N7B39_002514"
     CDS             complement(5917..6267)
                     /gene="chbA"
                     /locus_tag="N7B39_002514"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HGU2422478.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(6318..7676)
                     /gene="chbC"
                     /locus_tag="N7B39_002515"
     CDS             complement(6318..7676)
                     /gene="chbC"
                     /locus_tag="N7B39_002515"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HGU2422479.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVVALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANAL
                     KF"
     gene            complement(7761..8081)
                     /gene="chbB"
                     /locus_tag="N7B39_002516"
     CDS             complement(7761..8081)
                     /gene="chbB"
                     /locus_tag="N7B39_002516"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HGU2422480.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(8381..8719)
                     /gene="osmE"
                     /locus_tag="N7B39_002517"
     CDS             complement(8381..8719)
                     /gene="osmE"
                     /locus_tag="N7B39_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HGU2422481.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            8921..9748
                     /gene="nadE"
                     /locus_tag="N7B39_002518"
     CDS             8921..9748
                     /gene="nadE"
                     /locus_tag="N7B39_002518"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HGU2422482.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREACIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPQQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            9978..10865
                     /gene="cho"
                     /locus_tag="N7B39_002519"
     CDS             9978..10865
                     /gene="cho"
                     /locus_tag="N7B39_002519"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HGU2422483.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSQEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(10825..11400)
                     /gene="ves"
                     /locus_tag="N7B39_002520"
     CDS             complement(10825..11400)
                     /gene="ves"
                     /locus_tag="N7B39_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HGU2422484.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESADHFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(11603..12088)
                     /gene="spy"
                     /locus_tag="N7B39_002521"
     CDS             complement(11603..12088)
                     /gene="spy"
                     /locus_tag="N7B39_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HGU2422485.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(12418..13386)
                     /gene="astE"
                     /locus_tag="N7B39_002522"
     CDS             complement(12418..13386)
                     /gene="astE"
                     /locus_tag="N7B39_002522"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HGU2422486.1"
                     /translation="MDNFLALTLTSKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFKKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(13379..14722)
                     /gene="astB"
                     /locus_tag="N7B39_002523"
     CDS             complement(13379..14722)
                     /gene="astB"
                     /locus_tag="N7B39_002523"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HGU2422487.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNKASTRHRFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVVRQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTQEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(14719..16197)
                     /gene="astD"
                     /locus_tag="N7B39_002524"
     CDS             complement(14719..16197)
                     /gene="astD"
                     /locus_tag="N7B39_002524"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HGU2422488.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVGQACR
                     AARAAFPRWARLSFAERQAVVERFAGLLERNKGELTAIIARETGKPRWEAATELTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGCETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRVLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVFTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     IRMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(16194..17228)
                     /gene="astA"
                     /locus_tag="N7B39_002525"
     CDS             complement(16194..17228)
                     /gene="astA"
                     /locus_tag="N7B39_002525"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HGU2422489.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLVRTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(17225..18445)
                     /gene="astC"
                     /locus_tag="N7B39_002526"
     CDS             complement(17225..18445)
                     /gene="astC"
                     /locus_tag="N7B39_002526"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HGU2422490.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNESVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECASVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNIINHRYGLFNEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAVACEHFVSRGSS"
BASE COUNT         4720 a         4988 c         4405 g         4477 t
ORIGIN      
        1 tacaggtggt ttgaatgaat gtagcaatga aatagagaat ttcaggccat gtacctcccg
       61 gcaaaaccgg gaggtacgta atctttactc agtcacttcc ccttcctggc ggatctgatt
      121 tgcccaacgc tgggcggatt caggcacagt aaacgccggt gagcgcagaa atgactctcc
      181 catcagtaca aacgcaacat atttgccacg cagcatccag acatcacgaa acgaatccat
      241 ctttatcgca tgttctggcg gcgcgggttc cgtgcgtggg acatagctaa taatctggcg
      301 gttttgctgg cggagcggtt tcttcattac tggcttcact aaacgcatat taaaaatcag
      361 aaaaactgta gtttagccga tttagcccct gtacgtcccg ctttgcgtgt atttcataac
      421 accgtttcca gaatagtctc cgaagcgggg tctggctggt ggtctatagt tagagagttt
      481 tttgaccaaa acagcggccc tttcagtaat aaattaagga gacgagttca atgtcgcaac
      541 aaaacgaaaa gaacccacat cagcaccagt caccactaca cgattccagc gaagcgaaac
      601 cggggatgga ctcactggca cctgaggacg gctctcatcg tccagcggct gaaccaacac
      661 cgccaggtgc acaacctacc gcctcaggga gcctgaaagc ccctgatacg cgtaacgaaa
      721 aacttaattc tctggaagac gtacgcaaag gcagtgaaaa ttatgcgctg accactaatc
      781 agggcgtgcg catcgccgac gatcaaaact cactgcgtgc cggtagccgt ggtccaacgc
      841 tgctggaaga ttttattctg cgcgagaaaa tcacccactt tgaccatgag cgcatcccgg
      901 aacgtattgt tcatgcacgt ggatcagccg ctcacggtta tttccagcca tataaaagct
      961 taagcgatat caccaaagcg gatttcctct cagatccgaa caaaatcacc ccagtatttg
     1021 ttaggttctc taccgttcag ggtggtgctg gctctgccga taccgtgcgt gatatccgtg
     1081 gctttgccac caagttctat actgaagagg gtatttttga cctcgttggc aataacacgc
     1141 caatcttctt tatccaggat gcgcataaat tccccgattt tgttcatgcg gtaaaaccag
     1201 aaccacactg ggcaattcca caagggcaaa gcgcccacga tactttctgg gattatgttt
     1261 ctctgcaacc tgaaactctg cacaacgtga tgtgggcgat gtcggatcgc ggcatccccc
     1321 gcagttaccg caccatggaa ggctttggta ttcatacctt ccgcctgatt aatgccgaag
     1381 ggaaggcaac gtttgtacgt ttccactgga aaccactggc aggtaaagcc tcactcgttt
     1441 gggatgaagc acaaaaacta accggacgtg acccggactt ccaccgccgc gagttgtggg
     1501 aagccattga agcaggcgat tttccggaat acgaactggg cttccagttg attcctgaag
     1561 aagatgaatt caagttcgac ttcgatcttc tcgatccaac taaacttatc ccggaagaac
     1621 tggtgcccgt tcagcgtgtc ggcaaaatgg tgctcaatcg caatccggat aacttctttg
     1681 ccgaaaacga acaagcagca ttccatccag gtcatattgt tcctggtctg gacttcacca
     1741 acgatcccct gttgcaggga cgtttgttct cctataccga tacacaaatc agtcgtcttg
     1801 gtgggccgaa tttccatgag attccgatta accgcccaac ctgcccttac cataatttcc
     1861 agcgtgacgg catgcatcgt atggggatcg acactaaccc ggcgaattac gaaccgaact
     1921 cgatcaacga taactggccg cgcgaaacac cgccgggacc gaaacgcggc ggttttgaat
     1981 catatcagga acgcgtggaa ggaaataaag ttcgcgagcg cagtccatct tttggcgaat
     2041 attattccca tccgcgtctg ttctggctaa gtcagacgcc attcgagcag cgccatattg
     2101 tcgatggttt cagttttgag ttaagcaaag tcgttcgtcc gtatattcgt gagcgcgttg
     2161 ttgaccagct ggcgcatatt gatctcactc tggcccaggc ggtggcgaaa aatctcggta
     2221 tcgaactgac tgacgaccag ctgaatatca ccccacctcc ggacgtcaac ggtctgaaaa
     2281 aggatccatc cttaagtttg tacgccattc ctgacggtga tgtgaaaggt cgcgtggtag
     2341 cgattttact taatgatgaa gtgagatcgg cagaccttct ggccattctc aaggcgctga
     2401 aggccaaagg cgttcatgcc aaactgctct actcccgaat gggtgaagtg actgcggatg
     2461 acggtacggt gttgcctata gccgctacct ttgccggtgc gccttcgctg acggtcgatg
     2521 cggtcattgt cccttgcggc aatatcgcgg atatcgctga caacggcgat gccaactact
     2581 acctgatgga agcctacaaa caccttaaac cgattgcgct ggcgggagac gcgcgcaagt
     2641 ttaaagcaac aatcaaggtc gctgaccagg gtgaagaagg gattgtggaa gctgacagcg
     2701 ccgacggtag ttttatggat gaactgttaa cgctgatggc agcacaccgc gtgtggtcac
     2761 gcattcctaa gattgacaaa attccggcgt aaatatggat aaggtcacgt cccctgggga
     2821 gtgaccttac caccttactt acacatcaag ataactaccc aaccgataac cacgctcagc
     2881 aattgcatat ttcaacgacg ctgatgtcag cacttccagc tccgttaacc gtgggaaaca
     2941 gtaagcactc tggcgaatcg tattatcgac aaacgccgga tggcacatga cttccagcga
     3001 gcgttccccg cgatggctgg aatcatcaag cacttgcagg aacagtgcct cgctaatctc
     3061 ttcaccataa aatgcgctac tgaatccgtg cgaactgcgc agattagccg gtaaatcacc
     3121 agcgttagat agcggctgac ggtcgatgcg caacgcaatc ccctcttcag ccgcaaacct
     3181 ggcaacaata gggaaaatct gcgggaacat atgcacatga tgatggctat caagatgcgt
     3241 aggtttgcgt ccaaatagct caatgaaacg caaatactga ctggcaagct cctgagtaat
     3301 ttcttccagc ggtaaagcat cttcttctgc caactgccag atccattttc ccagcacacc
     3361 atcgcgggtt aaccccggca tagctgtcag tggcttgccc atagtaagga caaagttcat
     3421 ccctatggcc agactcggtt catcacgact caactgcacc gcatggtcaa tagcctgccc
     3481 attcaccagc gccgtcgtcg atgtgacaat cccattgcga caggcctcga taatgccgta
     3541 gttctgacct ttgcttaagc caaaatcatc ggcattaaca atcagtaagc gttccataat
     3601 cagcctcggt taatgtgctt ttttaagctc tgcgatgcag tcggcaaaat ttggcagcca
     3661 tttctcgtgc gccagaatca tctcgcgtgc cagcagctca gcatcgcgat cggaatgcac
     3721 caacggacta aggtttagcg ccagtaacac accgttaaat tctccgctaa gtgcggcgtt
     3781 gctggcagca atctcgaagc ctttaatggt gtgaatcagc cccatcactt tatcatcgaa
     3841 atgcgtaatg cgcggatgtg gcgtcgcgcc atcgcgcccc agcgtacagg tcatttctac
     3901 cgcccagtct gccggaatat tatcaatatg cccatgatgc gggatattaa cgtaatgttc
     3961 tgcttgctta tcgttgtaga tagcgttgat cacttcgcac gctgcatcag agtaataagc
     4021 cccaccgcgc tgttccagtt ctttcggctt aactttcaac tccgggtttt tatacagctc
     4081 aaaaagttgt ttctcgactt tctgtactac ctgcgctcgt gcgccgcctt tgtagtattc
     4141 gcccatttca atagccagca tctctttttg cttgaagtaa taaagcaaat aagaacacgg
     4201 caacagattc agagaacgaa ttaagccttc gctaaatggc agatcgaaaa tatttttaac
     4261 gccagatgct tttaactgcc cggatgccac accatcaagc aattcggcaa agcgcgactt
     4321 gccatttacc agcacatcct taatgaacac catatggttg aggccgaaca gatcgataga
     4381 taaatcatcg ctgtctttca gcatcagaac atcgcgaata aacatcttca tgccgatcgg
     4441 aatattacac acgccgataa agcgtttaaa tccggtatga cgataaacgg cttcagtgac
     4501 cattcccgcc gggttagtga agttaatcac ccatgcattc ggacaaagtt cttcgacatc
     4561 ttttacgatg tcaaaaatca ccggaatggt acgcagacct ttaaacagac cgcccgcgcc
     4621 gttggtttcc tgaccaagat aaccatgact taatggaata cgttcatcca gttcacgcgc
     4681 cggtaattgg ccaacgcgca gttgggtagt aacgaaatca gcatctttca atgcttcgcg
     4741 gcgatccagc gttttataaa gcttcatcgg gacgccagcg ttatcaatca tccgttggca
     4801 gagatcgaaa ataatatcca gtttagcttt gccaccttcg acatccacca gccataattc
     4861 gctgaccggc aattcgtgat aacgcttaat aaatccttcc agtaactccg gggtatagct
     4921 gctcccgcca ccaatagtga cgacttttaa tttctggctc ataatttctc ccttcagtac
     4981 agaatactga tatctggcat atctgccccc ccggacataa ataatccagc aacaggacag
     5041 atatgtgaat tgtcaggtat aacgacttac tgattaaatt cagtcaattt cttacgatag
     5101 ctcttaggcg taaaggacgt taatttctta aacgttttaa taaacaaact cgggctacta
     5161 taaccggcct caaacgcaat atccgtcact gaatagttgg tcatttccag ttgttttttg
     5221 gcaaaattaa tacggatttc attaataatc tgcattggcg ttttgccata atatcgttga
     5281 gtcgcccgcg tcaaatattc ctgtgatttg gctgacaacg tcaccatatt ctccagcgcc
     5341 gattcactaa actgctcttt atcatgcatc ttttctaccg tacttttcag ccactgcggt
     5401 acatcatcaa tcacctgttc ttcgcggtaa tgacgtaaac ggttaatgac ataaaaagta
     5461 accatctcaa caaactcttc cagccctgtt tcgcggaaat tcaatgaaga aatcactgtt
     5521 tccacatagg tgagaaaagc gttattggtc cggtatacct gcgaagctac aaagcaataa
     5581 ggcaacaatg gcaggtaatg ctgctcaaaa aagcgtttac tgatcccaac gttcaatatg
     5641 cgcgtggcac caaactcata aaaactttga tggtgcgaac ctaacggaat aaaaacaaaa
     5701 tcgccccgtt ccagtaacac gcgcttaccg ttaatctcct ggaaataacg cccggttaat
     5761 accagagtaa attcataata gtcgtgctga tgcagtccgc tgatactctc agttttgtta
     5821 tagataaaca catggaagtt tttgccatta aacaactgct gttctcgggc agtggcaatt
     5881 tccggcgcgt taatcactgg ctgcatcatc gactccttat gccttcagtt tttcatgaag
     5941 ctcaattaat tcagtaatca gttcacgcgc aagcatggac gtcattaaat gatcctgagc
     6001 gtggaccagc accagactca ctttcatctt accttcgccc gcatcgcctt caatcagttt
     6061 cgtctgtacc agatgcgctt cattcaatgc catccgtgac tgatccatca tggcttttgc
     6121 tgcggcaaaa tcgccctgct tcgcctgttt cagtgcggca tacgccaggc tgcgcgcttg
     6181 tccggagttg atgataagcc ccatcaccac ttcttccagc tcttcagctt ccgtttgcgt
     6241 atcgggaata ttatcgagat ccatcataca tcgttcctct cttttcttac cggcacgatt
     6301 acccgtaccg gcatcgatta aaatttcagg gcgttagcga tatcttcttc gctctcttct
     6361 ttatcaatcg cattttgtgc tttgttagcc accacaacaa agggcagata aattaacgtt
     6421 gcgataccaa ggttgaagag tgcaaccagc aatgcgacga cgctaccgtt ggtgttaaag
     6481 aaggctccca gaccggttgg catggtccac ggtgcaatat tggtcaccgg aggaataatg
     6541 cccatgtagt acgctgccag ggtgattgcc gccagaatcg gttgtaccag tacaaacggg
     6601 ataaacatca ccgggttcat gataattggc agaccaaaca gaatcggttc gttaatctgg
     6661 aagatgccgg acggcagcgc cagttttgcc acctgacgat aatcagcacg gcgagaggca
     6721 ataaagatag ccaggatcag gcctaaagtc gcaccactgc ctccaaggaa aataaaggag
     6781 tccagcatcg gcttggccca gatatggaag gtcttaccgg ctgccagcgc cgcttcgacg
     6841 gaaccatatt gctgataggt cgcgatattt tccagcgccc acggcgtcat aatgccgttg
     6901 tccagtgcgg tcagcgccag cgcgccatga ataccgaaga accagagcag tggaacaaag
     6961 atcacatagg cccagcccac cacgctaccc aacgatgcca gtggggttga gatggtatcc
     7021 ataatgatct gatggaagtt ggtgccccag gtattcaacg cccaggcaat aatccccatc
     7081 acggaaagaa taataaagcc gggaattaat gccgagaagg aacgcgatac tgaagcaggt
     7141 acgctgtcgg gcagtttaat gacccaattg cggcggacaa taaaggtaaa catttctgcc
     7201 accaccaggc caataataat cccggagatg atattcgccc cacctaacca gtttgcgcca
     7261 accgcatagg cctcaccgac actatatggg gtgacggtca taaatgctgc aacggataac
     7321 aacccagccg ccagcgcatc gactttacgc tcttctgcca gcgccatgcc aataaagaac
     7381 ggtgccatta aagacattat ccctaatgtt ccgttatata cgttgccgcc aatacctttc
     7441 agaccattaa gtgtttcaat ggttgaggcg tcgaggcgaa tacctaagga ataaaaaaac
     7501 gacccctccc caaagcttag aaaaacgttg ttaattaata caaacatggc ccccgcaagg
     7561 gttaacggca ttaagcgaat aaagccattt ttgattgcat taacgtgtgg ctgctttcct
     7621 attttaactg caaaagggag gagtaccttt tcaagcgatg caataacatt actcatagaa
     7681 aaataccctt aaaaaccgca atttaaatat tgcggtattg atttatgaaa taactctttg
     7741 acgggaaaat ttaaaataaa ttaatttgct gcggcttttt taatcgctgc aaccgcagcc
     7801 ttaagcacgc ctaaaccatc gactttgcca taaagcagcg agtcaattac ttcaaccggt
     7861 ttgttgggta acaaacgctg gatttcgggc aacatataag caatctgcgg ccctaataac
     7921 acgacatcgg cattctgacc tttttcacca gccagtgttt ccggaaatgc ttcaataatg
     7981 accggaactt catatttttc tgcctgtgcg cgcatttttg ataccagtaa agaggtagac
     8041 atgcccgcag aacaaaacag ataaatgtgt ttcttttcca taaaactgcc ctcatcgacg
     8101 attatctgtc agccagacac tccgcaagcc ttaacctgct tccatgctct gggtaacttg
     8161 cgaaaccaac atgacttttt ttgtggctga aaggagtata cggtatagac ctgagcaata
     8221 gtatttcttt gaccttataa attgtctctc cttgacctgc ggttatgacc ctcttcacat
     8281 ttcgggcaaa taattcgcga agataattaa gaactcaggc caaaaaaaaa ccggcgcaat
     8341 ggccggtttc cgttgttact caagctttca gacgaagtga ttacttcgca gcctgtggat
     8401 cagtgtcgta ttcagcacag gtctgataac cggagttgat gacatgtccg gtatcatcta
     8461 acgcgacaaa gtaggtttct gctttaccat cacgttgacc caggatgtag gtctggcaag
     8521 taccacgagc atggatcatg ctcacttcag acgaaggttt acccgcaatt tgtgcaacct
     8581 gcgcccggct catgcctttt ttgacgtctt tcaccacagg ctgtacaaac tggtctttgg
     8641 tacgatcata agccgtacaa cccgccagca tggttaatac cgccgctgca ctcagaattc
     8701 ctgccatatt cttgttcata ttccgtccta ttgtttatca gcgtgttaga taagcctgga
     8761 atacattggg cgctttttca agcccatgaa cgaaacggct ccgctttcag aggattcctg
     8821 tatgacgttt taaccaccat tcagcccgct gtcgcttgtc gtttcagtag caacgggtta
     8881 gctttaagga agttttgtct tttctgtctg gaggggttca atgacattgc aacaacaaat
     8941 aataaaggcg ctgggcgcaa aaccgcagat taatgctgaa gaggaaattc gtcgtagtgt
     9001 cgattttctg aaaagctacc tgcaaactta tccgttcatt aaatcactgg tgctcgggat
     9061 cagcggcggt caggactcca cgcttgccgg taagctgtgc cagatggcga ttaatgagct
     9121 gcgccaggaa accggcaacg aatcactgca atttattgcc gtacgcctgc cctatggtgt
     9181 tcaggccgac gaacaagatt gccaggatgc cattgccttt attcaaccgg atcgcgtatt
     9241 aaccgttaat atcaagggcg cggtattggc tagcgagcag gcattgcggg aagcatgcat
     9301 tgaactgagc gattttgtcc gtggcaatga aaaagcgcgt gagcggatga aagcacaata
     9361 tagcattgcg ggtatgacca gcggtgtcgt ggtgggcacc gatcatgcag cagaagccat
     9421 taccggattc ttcactaaat atggtgacgg cggtacggac attaatccac tgtatcgtct
     9481 caacaaacgt cagggtaaac agttactggc agcattaggt tgcccggaac acctttataa
     9541 gaaagcgcca acggccgatc tggaagatga tcgcccttct ctgccagatg aagtggcact
     9601 cggcgtgacc tatgacaata tcgacgacta tctggaaggg aaaaacgtac ctcaacaggt
     9661 cgccagaaca atagagaact ggtatctgaa aaccgaacat aaacgccgtc cgccaattac
     9721 cgttttcgat gatttctgga aaaagtaata atttgcacct attggattgt gcaaaaaaga
     9781 gtaatttgtt cacgccggat gcggcgtgaa cgccttattc gacctacaaa actatgcaaa
     9841 ttcaatacat tgcaggagtc gaataggcct gacaggcgta gcacgtcaga cggtgtaacc
     9901 tttgtcatcg acccgcctct tttttaatcg cttcccggct gttacactgg atagataacc
     9961 agcattcgga gtcaacagtg gtacggcgtt taacttctcc gcggctcgaa tttgaagctg
    10021 cggcaattta tgaatatccc gaacatttac gttcattcct taatgactta cccacccgac
    10081 caggggtgta tctgtttcat ggcgaaagtg acaccatgcc gctctatatc ggcaaaagcg
    10141 ttaatatccg cagtcgggtg ctttctcatt tacgcacccc ggatgaagcc gccatgctac
    10201 ggcaatcccg gcgaattagc tggatctgca ccgcaggcga aatcggcgct ctgctccttg
    10261 aagcgcgatt aatcaaagaa caacagccgc tgtttaataa acggttgcgc cgcaatcgcc
    10321 agctctgtgc cctgcaatta aatgaaaagc gcgtcgatgt ggtgtatgcc aaagaggtgg
    10381 atttttcacg agcccccaac ctgtttggcc tgtttgccaa taggcgcgca gctttgcaag
    10441 cattgcagag catcgctgat gaacaaaaac tttgttatgg tctgttggga ctggaaccgt
    10501 taagtcgcgg tcgtgcgtgt tttcgttcag cgctaaaacg ttgcgccgga gcatgctgcg
    10561 gtaaagagag ccatgaggaa catgcgctac gcttgcgcca gtctctggag cgtttgcggg
    10621 tggtgtgttg gccttggcaa ggggcggtgg cgctgaaaga acagcacccg gaaatgactc
    10681 aatatcatat tattcaaaac tggctgtggc tgggggcggt taattcgcag gaagaagcga
    10741 caacgttaat tcggacaccc gccgggtttg atcacgacgg ttataaaatt ctttgtaagc
    10801 cgctgctttc cggtaactat gaaattactg aacttgatcc ggcgaatgac cagcgagcca
    10861 gttgatttca ctgaacaaca gtttaccttg cggttgtaaa agccgcaggg tgtgcctgcc
    10921 atcgaaccag cacgcgccct ggtcggtagt gagcagtttg tcgcccagtt gccacgcccc
    10981 gttaatcaca aacaccaccc cgccgcgtga gccaaaggta gtaaacgtac gttcggcaat
    11041 tctgactttc gccttacaga catccaggcg cgtcataata ttgaaatcca tcgacatctg
    11101 ccctgccgtc agtttcgctt ttaccacctg gtccgccgca aaggcaaaag gctgtaacgg
    11161 ttttaaggta tggttaaagt ggtctgcgct ttcaaggaac atctcgccgc cttccagcaa
    11221 cgtcactatc ctttccatac caggaaacaa agaaaactcc ccattcgccg cgatggatgc
    11281 aatgctggca cgccagtaaa aatcacgttt tgccggtggg aaagtgcaaa tttcgcgcgt
    11341 ttcaccggca gcgtttcgcc acaaattcac cgacatttta cgcatatcaa agtattccat
    11401 actcgctccc ggcccgttct tttcattgtt atcgttcagg taaccgtgga tggttatcgg
    11461 caactcgggt gatttacgtt agtggtgatc aggctttttt tacaactgtt tgagaagaga
    11521 aaagaaaacc gccgatcctg tccaccgcat tactgcaagg tagtggacaa gaccggcggt
    11581 cttaagtttt ttggctgaaa gattattcag cagttgcagg cattttacct tttgccgctg
    11641 gacgttctgt cagacgcttc tcaaaattag cattaaattg ctttttctgt tccggcgtca
    11701 ggatgttgta aattttgttc tgggtttcca tgtgcgccag catgttagct ttgcgctgtt
    11761 cttccatttt tgcgatctgc gcttcagctt ttgctttatc gaaggtatcg ctggcaatga
    11821 tgtcatgcat tgcgcgacgt tcttccagcg gcggacgttt catctggtca cgctggcctt
    11881 tcatgatttc gcgaatctgc tgtttctgcg cgtcggtcag gttcaggtct ttgaacatca
    11941 tgtcctgatg cggaccgaac ttgcctttgt ggtgcatcat cggcttcgcg tcagccggtg
    12001 ctgcggtagt ggtgtctgcg gcatgggcca ggttagccgc gccaagagcc agggtagagg
    12061 caacaaacag tgcagttaat ttacgcatat tctatatcct tcctttcagt tatttattac
    12121 ggctttctta agtagcgtgc cgtgttgacg agattaactt tactgacttt agcgtcaatt
    12181 aatcagagca acggtaaaac aatgaaagtg taaaaaacac ttttgcgcca attatggaga
    12241 aaaaaagaaa atttgatgga gagtgatgag agaatattac aacaccatga ttttgcagag
    12301 attatgaaga actataccgg atgactggtg ataaataaag caaataacca ggattaatct
    12361 gtattaattt ataagaaggt aacttaatac ccgcagaaag atttctgcgg gtaagtatta
    12421 gcttattttt tcgagcatta atcccgcgcg taatcccagc gctaccaacg gattagggaa
    12481 taacacatac tctacatcat gggttacggt aaaacgttcc tcaccgtcct gcgccagcaa
    12541 cgttcctttc ttaaacggca taaaattcag cgtgtcactt gccatatgca tttcgaagga
    12601 cggcgagtgg cgagtaattt gcgaaaccac ccgataacgg agcggcggtg ttctcacgat
    12661 accgacactc tcaccagata gcagcgcagc gattgcgctg gcagttactg caaactggcg
    12721 aagatcgttt tgcccaaagg gcaacgcttt gccaagttcc agcgtacagg ccagcgcgcc
    12781 aaaatgtctg gcgctgaaat gggtaaacgt accaccaggc tcctgatgga acaccagcgc
    12841 ctccagcccc gccgcaccca gccacgtcag gaatttctcg tcccagggaa tgtcgcgttg
    12901 cggtaataca ccgaactgcg gatgcaagga gccacgaatt gcggtatgta gatcaaggtg
    12961 ccagcgcaca gattctttgc cctggtcata aaaatcttcc aggcactgtt ccagttcgcg
    13021 cgcccgacag gtttccccgc tttcagcaaa cagctgccaa cgaccgccaa acattcggtt
    13081 catatcgcta tggcaataac gtttcccttg cttcagcgca ggaggattcc cgaggatcac
    13141 cagcaaccgc caccgtaacg ggatctcgcc gtgagatatc gcgccaagca atgcatcaag
    13201 catctccaca ggtgccgtct cattaccgtg gatccccgca gaaatcacca gagcgccttg
    13261 cggtggcgtt aatggcgtca gttccagcac accatcgccc agccagcgcc agcgaacgcc
    13321 gttgatttct cgctcggtga tgacaggttt tttactcgtt aaggtcagag caagaaaatt
    13381 atccattgcc ccctccctcg cgctggaacg gataaaccga accgagattc agtaattggc
    13441 tcaatacatc cagtgcttcc cgcccttcgc gcagcaattg cgggtcggcc agatcggcag
    13501 cagtaagacg atcgcggtag taacgatcca cccagtcatt gagcgcatta aacaaggtat
    13561 cgttcatcat caccgccgga ttcaccgccc ggcgttcttc ttgtgtcaat accacccgca
    13621 accgcaggca cgccgggcca ccgccattcg ccatgctttc acgtaaatca aagactttta
    13681 gttcgctaat cgggttgtca gcggcaagga gttcattgag ataaccccat actccggcgt
    13741 gttcccgaca ctcctgaggc agcaccaaca tcatggaacc atcatcgcgg ctcaacagtt
    13801 ggctgttaaa cagatatgta gacaccgcat ccgacacgga aacctgagtt gccggaactt
    13861 ctatcgccat aaaaccgttc acccgcgcac gcaggtttgc cagtaactgt gactggcgag
    13921 cgaacgcctg ttggtggcaa aacagcacct ggcggttact cacggcaatc acgtcattat
    13981 gaaaaacgcc ctggtcgata acgtccgggt tttgctgggc gaaaatcacc tgttggggat
    14041 tcacctgatt cagccttgcc accgcctcgc tggcttcgcg agtctgtcgc gccggataac
    14101 gggaaggccg ggtatcattg ccctcttctc gcccgtagac aaaaagttgc atacccggtt
    14161 caccgtaatg accgccgaga cgattgtggt ttgccgcccc ctcatcaccg agcaaggcta
    14221 cctgcggcaa cgccgaatgg acactaaatt tctcttcgtc gttaaaaatc gcttttaaca
    14281 gcgattcagt gacgggcgct tccagcgaac ggtgaaattt attgttcagg ttagcaacgg
    14341 tgagatgcac tttgccatcc agcgtatcgg cagatggcgc gatcgttgcc gcattggcta
    14401 cccacattgg cgaagcggaa ctgacgctgg aaagccagtg cggtgcctgg cgtacaacct
    14461 tttccagtac ctgctcatcg ctaccactga atcccaactg acgcagcacc ggaataaacg
    14521 gacgctcgtg aggcgggatc acggcctggg ggaatcccgc atcggcaagg gttttcattt
    14581 tcagtaagcc ctgttttgcc gccagtcgcg ggttagagac ctgaaaacgg tgacgggtag
    14641 aggctttatt accaaacgac aggcccgcgt aatgatgcgt cagccctacc agcccgtcga
    14701 aattaacttc ccaggcgttc atcgcaccac ctcatcggaa aaatccagcc cggggttaag
    14761 cgttgcgggc aaagttaacg agtccgactc caggctcgcc ataggccatg cgcagtaatc
    14821 tgcggcatac caggcgctgg ggcgatggtt accggatgca ccaatgccgc cgaatggcgc
    14881 ggtactggca gcaccagtaa gcggtttgtt ccagttaaca atccccgccc gcgcctccag
    14941 caacagttga tcgaattttt cccgctcggg ggaaaccaga ccgcaagaga gtccgaagcg
    15001 agtgttattc gccattcgaa tcgcttcctc gaaagaatca taacgccaga cgcgcaataa
    15061 cggtccgaac acctcttcat ctggtacgcc agcaacgcct gtcatttcaa tgatccccgg
    15121 cgtcagcaac gatgtccctg cttgtaataa gcgcggcgca agcagggttc gtccacccat
    15181 cgcttccagt tgctgccagg cagtaaacac ctgctgtgcg gcctgttcag aaatcagccc
    15241 gccaataaac ggctgcggtt cgtcatccca gttgcccggc gttaatcgct ggctgacggc
    15301 aaccagacga gcaagaaatg catcgccctg cgccccgctt ttcagcaata cacggcgggc
    15361 gcaggtgcag cgttgcccgg ctgtaacaaa cgccgactga atggtcagat ggacagccgc
    15421 gtcgatatcc gccacctcat cgataatcag cgggttattg ccgcccatct caagggcgag
    15481 aattttctcc ggctgaccgg agagctggcg atgcagctgg tagccggtat tggcgctgcc
    15541 ggtaaacagc aagccgtcga gatcctccag cgcactcagc gcctgacccg tttcacaccc
    15601 gccctgcacc aggttcagca cgcctggcgg caagccagcc tgctgccata aacgcattac
    15661 cgcttcgcca ctccacggtg tcagttcgct gggtttaaag ataatggtgt tacctgccag
    15721 caatgcggga acgatatgtc cgttcggcaa atgaccaggg aaattatacg gcccaaacac
    15781 cgccagcacg ccgtgcgggc gatgtcgcag gctcgccgca ccgtccggca tttcactacg
    15841 ctgctcgccg gtacgaacgt gatacgcctt aattgatatc gcgattttat tgatcatcgc
    15901 cgtcaattcg gttgccgcct cccagcgcgg cttgccggtt tctcgggcaa tgattgcggt
    15961 taattcgcct ttattccttt ccagcaatcc ggcaaagcgt tcgacaacgg cctgacgttc
    16021 agcaaatgag agccgcgccc agcgcggaaa cgccgcacgg gctgcccgac aagcctgccc
    16081 gacctgagcg gcatcggcat cattgccttg ccataacacc tcgcccgata ccggattacg
    16141 cttcacacgc gatgcaccct ggcccgttat ccagtcaccg ttaatccata aagtcatgct
    16201 gttttctcct ctgcgcacag gcgcaccaga cgaacgcgat cccctgcatg gcatttgagg
    16261 gcatccagtt gtgcggcggt taaaatcaaa cgctcggttg ccggatcggt acgcaccagc
    16321 accacgcgga aatggtgata attttcattg gcgaccaggc aggccggaaa atcgccctgc
    16381 gcaggctgcc cttctgccac ttccaccagc cgacttttac ggatggcgcg cacgcggtcg
    16441 atgtcacact caagcgtcgg cccaccgtcg aagatgtcga tatagttgcg gtagcgaaaa
    16501 ccttctttct ccagcaccgc gcgggcaggc gcggtttgcg gatgtacctg accgatgacg
    16561 tcctgggctt cctgggataa aaagtgcgta tagatcggat gtttcggcat cagttctgca
    16621 ataaatgcct tttgcccggt gccgcagaga aaatcggcgc ggctaaaatc catcgaaaag
    16681 aagcgtttac cgaggctttg ccagaacggt gaatagccgt gttcgtcaat caccccgcgc
    16741 atttcagcaa ccactttgtc attaaacttg tcgcgaaaag ccgccataaa cataaagcgc
    16801 gatttcgaca gcaaatagcc gttgccctct ttgcgccagt ccgggtcgag aaacagcgtg
    16861 cacagctcgc tgctgccggt gtgatcgtta ctgagaaaca acgtcggcaa tgcgttatag
    16921 acattcagct cttttgaggc gtgaaccaac gtgccgacgc gatagttgta ccagggatcg
    16981 ttcagcccaa ctgccacctc aatggcgcaa atccccgcca cggtgcctgt ctcgctatct
    17041 tccagtacaa acacataacc ctgctcactt ttgggcagtt caccttgcca ggttttgatt
    17101 gccctttcga tacgcgccga aagcgtggct tcattggcgg gaagcgacgt caggccgccg
    17161 cccgttttgc tggcaagctg catcagcgcc gagacatctg agcgctcaac gggacggatg
    17221 accatcatga tgaacctcgg ctaacaaagt gttcgcaagc aactgcaaag cgatccagtc
    17281 cggtcgtcac ctcttcttcg ctgacattga gcgcaggcgc aaaacgcacc acgttgccac
    17341 ccgcaatcag taccatcacg cctgctttcg ccgcttcctg agagatctgt ttcgcttgcc
    17401 cggcgtaatc ggcattcagt acacagccaa tcagcaaacc taagccgcga acttcattga
    17461 acaaaccata gcggtgatta ataatattaa gacgctcaac aaaccagtcg tgacgttgct
    17521 taaccccgtt aagcatctct ggcgtattga tgagctccag cactttgcct gccaccgccg
    17581 aggccagcgg attgccgcca taggtggtgc catgagtgcc aacggtcatc acgctggcgc
    17641 actcttcggt tgctaacaac gcaccgaccg ggaaaccgcc gcccagagct ttggcggtag
    17701 ttaacagatc aggcgtcacg ccgtagtgca tataggcata cagttccccg gtacgcccga
    17761 cgccggtttg tacttcatca aaaatcaaca gcgcattgtg gcgatcacac aattcacgca
    17821 gaccttgtaa aaatgcgttg ctggctggca ccacaccgcc ttcaccctgg atgggttcga
    17881 caatcaccgc acaggtagag tcgtcaatca gcgcgctggc agagttaata tcgttatatg
    17941 cagcatgacg gatatctggt ggcagtggcg caaaatcctg tgaataggct ggctgcccac
    18001 ccgcactaac agtaaacagc gtgcgaccat gaaacgcatt tttaaacgcc acgataccgc
    18061 tcttatggct gccgtagcgg tcgtgagcga atttacgcgc cagttttagc gccgcttcgt
    18121 tggcttccgc accggagtta caaaagaaaa cgcgatcggc aaacgtggcg tcgatcaatt
    18181 ttttcgccag tcgcagtacc gactcgttgg tgtaaccgtt gccggtatgc cagaacttac
    18241 tcgcctgttc gttcagcgcc tcacgcagtt ccggatgcgc atggcccagc gcgttcaccg
    18301 caatgccacc cgcgaagtcg atatactctt tcccctgctg atcccacaag cgcgaacctt
    18361 cgccacgtac cggtataaac ggtgccggag cgtaaacagg tatcatccat tcatcaaagt
    18421 tttcacgcgt aattggctga gacatagcga cctctacagt aaatagctcg ttatttatat
    18481 gttaataata agtaatgttt tcgctgtaaa tgtagattgc agggttcgtg ccagccagtg
    18541 atgaaagtgc ataaacggcg gaggctaact ggaaatcaag gagttataac
//
LOCUS       DBFIFI010000076        18444 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.076, whole genome shotgun sequence.
ACCESSION   DBFIFI010000076 DBFIFI010000000
VERSION     DBFIFI010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18444
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.076"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            1..556
                     /locus_tag="N7B39_002527"
                     /pseudo
     CDS             1..556
                     /locus_tag="N7B39_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012896819.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
     gene            659..1162
                     /locus_tag="N7B39_002528"
     CDS             659..1162
                     /locus_tag="N7B39_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HGU2422491.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKKFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(1229..2686)
                     /gene="nuoN"
                     /locus_tag="N7B39_002529"
     CDS             complement(1229..2686)
                     /gene="nuoN"
                     /locus_tag="N7B39_002529"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HGU2422492.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2693..4222)
                     /gene="nuoM"
                     /locus_tag="N7B39_002530"
     CDS             complement(2693..4222)
                     /gene="nuoM"
                     /locus_tag="N7B39_002530"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HGU2422493.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4453..6294)
                     /gene="nuoL"
                     /locus_tag="N7B39_002531"
     CDS             complement(4453..6294)
                     /gene="nuoL"
                     /locus_tag="N7B39_002531"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HGU2422494.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6291..6593)
                     /gene="nuoK"
                     /locus_tag="N7B39_002532"
     CDS             complement(6291..6593)
                     /gene="nuoK"
                     /locus_tag="N7B39_002532"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HGU2422495.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6590..7144)
                     /gene="nuoJ"
                     /locus_tag="N7B39_002533"
     CDS             complement(6590..7144)
                     /gene="nuoJ"
                     /locus_tag="N7B39_002533"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HGU2422496.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7156..7698)
                     /gene="nuoI"
                     /locus_tag="N7B39_002534"
     CDS             complement(7156..7698)
                     /gene="nuoI"
                     /locus_tag="N7B39_002534"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HGU2422497.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7713..8690)
                     /gene="nuoH"
                     /locus_tag="N7B39_002535"
     CDS             complement(7713..8690)
                     /gene="nuoH"
                     /locus_tag="N7B39_002535"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HGU2422498.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8687..11413)
                     /gene="nuoG"
                     /locus_tag="N7B39_002536"
     CDS             complement(8687..11413)
                     /gene="nuoG"
                     /locus_tag="N7B39_002536"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HGU2422499.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSLE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQCRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11466..12803)
                     /gene="nuoF"
                     /locus_tag="N7B39_002537"
     CDS             complement(11466..12803)
                     /gene="nuoF"
                     /locus_tag="N7B39_002537"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HGU2422500.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12800..13300)
                     /gene="nuoE"
                     /locus_tag="N7B39_002538"
     CDS             complement(12800..13300)
                     /gene="nuoE"
                     /locus_tag="N7B39_002538"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HGU2422501.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHANLTPEAIPE
                     LLERYK"
     gene            complement(13303..15105)
                     /gene="nuoC"
                     /locus_tag="N7B39_002539"
     CDS             complement(13303..15105)
                     /gene="nuoC"
                     /locus_tag="N7B39_002539"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311197.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HGU2422502.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPARVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15199..15861)
                     /gene="nuoB"
                     /locus_tag="N7B39_002540"
     CDS             complement(15199..15861)
                     /gene="nuoB"
                     /locus_tag="N7B39_002540"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HGU2422503.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15877..16320)
                     /gene="nuoA"
                     /locus_tag="N7B39_002541"
     CDS             complement(15877..16320)
                     /gene="nuoA"
                     /locus_tag="N7B39_002541"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HGU2422504.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16951..17889)
                     /gene="lrhA"
                     /locus_tag="N7B39_002542"
     CDS             complement(16951..17889)
                     /gene="lrhA"
                     /locus_tag="N7B39_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HGU2422505.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPISAPEGDDSLLIERDIE"
BASE COUNT         4612 a         5125 c         4736 g         3971 t
ORIGIN      
        1 gccaacgccc cgacacgtaa ttcctgagca attagaaaaa acagttcgtt atcttacaca
       61 aactgtgcat ccacgtaatg ccgacaatat tgataatctg aataggtccg tagagtatat
      121 aaaaaaaggt ctttgtcagt agcggtgcca gagttaccgc gcagggcgtc cccaattggc
      181 tattacgact ctgcgcctgg tagccaggat tacccttacc cgcaatgtcc tggctttatc
      241 ccgatcgggg ggactttatt gcctggtcgg cagaatacag gatatcaacg ccgttcgtca
      301 ggtaaaaacg gcattgttat catcccggga tttatctgtt tattctatga atgccccatg
      361 gtttattcac ggtattgatt tctctgacca ccttaattat tggcaacacg atattctcgc
      421 cgtaatgatt actgacaccg ctttttatcg taataaacaa taccacttgc ccggtgatac
      481 agcagacaga ttgaattatc agaaaatggc tcagatggtg gatggtgtta taactttgtt
      541 acacaacagt aaataataaa aatttctcga ggcgggtcca gaaacagcga catgtacatt
      601 ttattttccc tcgtctgttt taagatgcga aacataaaat tcaatggagg aaaaataaat
      661 gggaatgatt ggttattttg cagaaattga ctcagaaaag attaaccaac tgcttgagtc
      721 tactgaaaaa cctttgatgg ataatataca tgacacgctt tcaggattac gccgactgga
      781 tattgataaa cgctgggatt ttttacactt tgggctgaca ggcacctctg cgttcgatcc
      841 agcgaagaat gatccgctga gtcgtgccgt gcttggtgaa cacagcctcg aagatggtat
      901 agatggtttc ctgggattga catggaatca ggagttagcc gcgacaatcg accgactgga
      961 atcactggat cgaagtgagc tgcgtaaaaa gttttccatt aagcgactca atgaaatgga
     1021 gatttatccg ggagtgacct tttcagaaga gttagagggg caactctttg cttcgatcat
     1081 gcttgatatg gaaaagctga ttagcgcgta tcgacgtatg ctgcgacaag ggaatcatgc
     1141 gctgacggtt attgttgggt gatggtgtca gggcaccaaa aaaaaccgcc gaacatgtcg
     1201 gcggctttct gacttacaaa ctgatagctt acatcagcgg cattgctaac cgcacaatac
     1261 taatcagcgg ttgtggccat acacccagca ccagtaccaa cagtgcagaa atcagcacca
     1321 cgataccgcc cgcgctgtac tgccagtttg acggtgcatc gcgacccggt tgttccggtg
     1381 cgtgcaggta caggctcacc gccacgcgca ggtagtagta gaggccgatt gccgaaccga
     1441 caaccacggc acccaccagc caccacaggt gtgcctggac accaaccgcc agcacgtaga
     1501 acttaccgat aaagcccagc gtcatcggga taccggccag agacagcatc atcaccgtca
     1561 tcaccgccgc gaggatcgga cgatgccaga acagaccgcg gtaagagaac agggaatcag
     1621 catccgggcc acgatacggg ctggacatca ggctgaccac gccgaacgcg ccgaggctgc
     1681 tgaacagata accggccagg taaaccccta ccgcttccat cgacatctcg ccagtttgca
     1741 gcgcaatcag cgccaccagc agatagccga ggtgagagat agatgagtaa ccgagcagac
     1801 gtttgatatt ggtctggctc agcgccatca ggttaccgaa gatgatggag gcaaaggcga
     1861 taatcgccag caccacccga atcgcttcgc tgtcacccac cggtgcgtag aggaacagac
     1921 gcatcaccac accgaagata gcgattttgc tcgccgtcgc caggaaagtg gaaaccggcg
     1981 caggcgcgcc ctggtatacg tctggcgtcc acaggtggaa cggcaccaga gagagtttga
     2041 agccgaggcc aacaatcatc aggccgaaac ctgccagcaa cagcggctcg tttagcatac
     2101 cgtcgccgag gtttttaccc agcgcgacaa acgacaggtc gccagactgc gcatacacca
     2161 gcgccatacc gaacagcagg aaagaagacg ctgcggcaga aaggatggtg tatttgatac
     2221 tggcttccag cgaacgtttc tggcggaaag cgtaaccgac caggccaaac agcggcaaag
     2281 agatcagttc aataccgagg aacagagacg ccagatggtt tgcattcgcc agcaggatcc
     2341 cgcccagcgc ggcaattaac accaacaggt agaactcatc cttgttgtcg ttatagcctt
     2401 caagccacgg gtaggcgaaa gtacaggtgg cgaggctcgc caacaatacc agcccggtgt
     2461 aaagcatggc gaaaccatca acgcgcatca gcggcgtaac gtccatagcg cccgcctggc
     2521 caacaaacca gagcgaaacc agcgccgcgt taagcccaat aaccgagagc gtagcgttga
     2581 ggaaatgatt gcgtcgccac gcaatggaga gcatcacaac caccaccgtc aagccgacga
     2641 tcagcaacgg tagcagtgcg atcaggtttt gtggagttat tgtcatggcg atttacggcc
     2701 ttgtagtagt aacggaatta acaaaccact gctggatatt gccaatcgcg gagtgcgagg
     2761 tatccagaat cggctgcgga tagaagccca gcagtaccag cagcaccacc agcaacagga
     2821 tcataaacag ctcacgcagc gacatccctg gcagttcctg gctggcaatc tggcttttcg
     2881 ctttaccgaa gtaagcgcga tgtaacatcg ccagcgaata aacagatgca aagaccagcc
     2941 caaaggtaga gataacggta atcaccggga caacctggaa gctgccgaac agaatcataa
     3001 attcgccgac gaagttaccg gtgcctggca tcccgagcgt tgccaccgca aagaacagtg
     3061 acaaggctgg cagccatttc atcttgctcc acagaccgcc catcatgcgc atgtcgcggg
     3121 tatggatacg ttcataaagc tgaccacaaa gaataaacag acccgccgct gacaagccgt
     3181 gcgcaatcat ctggattacc gcgccctggt aggccaactg gctgccggtg tagatagcaa
     3241 tcagtacgaa gcccatgtgg gaaaccgagg tgtaggcgat cagacgtttg atatcggtct
     3301 gggcgaaggc catccacgca ccgtagaaga tgccgataac acccagccac atggcgattg
     3361 gcgcgaactc tgccgacgcg tttgggaaca gcggtaggga gaaacgcagc agaccgtaag
     3421 cggcggtttt cagcaagatc cccgcgaggt ccacggaacc ggcggtcggt gcctgtgagt
     3481 gcgcatccgg cagccagcca tgcagcggaa ccaccggcat tttcaccgca aacgcgatga
     3541 agaagcccag catcaacaga tattccacac cactggacat tggcgtattc agcagctctt
     3601 catagttgaa ggtccagacg ccggtcgcat tgtagtgaac aaaaaccagc gccaggatgg
     3661 cgatcaacat caccagacca ctcgcctggg tgtaaatgaa gaacttggtt gccgccgtga
     3721 tacgcgtttt accatcagag gctttatgcc cccacagtgc gatcaggaag tacatcggca
     3781 ccagcatcat ttcccagaag aagaagaaca ggaacatgtc gatggcaagg aacacgccga
     3841 taacgccgcc caggatccac atcaggttga ggtggaagaa gccctgatat ttttcgatct
     3901 ctttccacga acagagtacc gccagcacac cgagcagacc ggtcagcacg accatcagca
     3961 gcgacagccc gtcaatggcg agatggatag agataccaaa acgcgggatc cacggcatgt
     4021 cgaattcaga ctgccactgc ggaattccgg cggattgcgt cagtgaataa ccgccctgca
     4081 accacagttg cagcgacagc gccagcgtca atcccatggt gatcagcgcg atccagcgcg
     4141 gcaccttgac gccaaagcgt tcggtctgcc agcacaggaa gccgccgata aaggggatta
     4201 gtattaacca gggtaataac atagctaagt atgtccctta tatttactcc tgctcaggct
     4261 atctcactcg ccagcccgaa tcccggcagt gctcgcaatc ctcacgtact taagtacgct
     4321 ccggttgctg tgcgctggcg gtatccggtc tgacttcgcc gatgacgcct gtacttcagg
     4381 aacagttacc taaaatttat tcttagttca cagacccggc gcaacgccgg gtatattcac
     4441 tcagccaaca actcaacgca gtaccatcaa cagtgccagc accacgaccg caccgatgct
     4501 catggatgcc acataccagc gcagataacc gttctcgctt aacagcaggc ctttacctgc
     4561 aaagcgggaa aggacagccg ggatgttcat cattgagttc agcggatcgc gtttcagcaa
     4621 ccaggcaata cccaggaacg gcttgacgaa cactttgtcg tacagccagt caaagcccca
     4681 ggcgttgtac caccaggtgc ccagcagacg gcccggcgca ctgttggcga tggaggtcac
     4741 cagagtacgt ttacccagcc acagccaggc tgccagcaga atgccgacca ccgcgaccac
     4801 gccagaggta atttccaggg tcaacatgct gccgtgcgcc agttccgtcg tttgcggaag
     4861 cacgccctgc agcggcggta caatcagtgc gccaacgaag gtggaaagga tcagcagcac
     4921 aatcagcggc aggctgtgag ttaccccttt cacggcgtga gcgtgaattt gttcttttcc
     4981 gtggaagacg atgaaaatca tacggaaggt gtagagcgag gtcataaacg caccgaccag
     5041 acctgccacc atcagattga tatgaccatt cgccatcgca cccgcgagga tctcatcctt
     5101 actgaagaag cccgcagtga ctagcggcag tgccgacagt gctgcgccgc ccaccaggaa
     5161 gcagagataa accagcggaa tagatttacg cagaccgccc atcttgaaga tgttctgttc
     5221 gtgatggcag gcgagaatga cggaaccgga tgccaggaac agcagcgctt taaagaacgc
     5281 gtgggtcatc aagtgaaaaa tcgccgcatc ccatgcctgc acgccaagcg cgaggaacat
     5341 gtagccaatc tggctcatgg tagagtaagc gagaacacgt ttgatgtcgg tctgtaccag
     5401 cgcggcaaaa ccggccagca gcagcgtaac cgccccaaca atgcccacca gatgcagaac
     5461 ttccggcgtc atcaggaaca ggccgtgggt acgggcgatc aggtagacac ccgcggttac
     5521 catcgttgcg gcgtggatca gcgcggagac aggcgtcggg cccgccatcg cgtcggcaag
     5581 ccatgtctgc aacggcaact gcgcagattt accgaccgca ccgcccagca gcatcagcgt
     5641 cgcccacatc agcatgttat tgccgtcagc aaagtgcgct ggtgccagtt ccaccatttc
     5701 gcggaagttc agggtgccca gttcgttgta aagaatgaac agcgcgaaag cgaggaacac
     5761 gtcacccaca cgggtcacga cgaacgcttt cattgccgct gcgccattct tcggatcggt
     5821 gtaatagaac ccgatcagca gataggagca caggcccacg ccttcccagc cgaggtacat
     5881 cagcagcagg ttgtcggcaa gcaccagaac caccatgctg gcgataaaca ggttggtgta
     5941 agcgaagaag cgagagtagc cctcttcacc gcgcatatac caggaggcgt acatgtgaat
     6001 aaggaaaccc acaccagtga ccaccgagag catggtcagc gacaggccgt ccagcaccag
     6061 gttaaaaccg atgttaaagt cgcctaccga catccacgtc cacagcggct ggctgtatgc
     6121 ctgctcgccg ttagcgaaga agtcaacgcc aataaaggca gttaccagcg ccgccaggcc
     6181 cacagagcca acgccgacga tcgccgagac gttttcagac cagcgcccac gggagaacgc
     6241 cagcaggacg aagccaatca atggcaaaat aatggttaag gcaagcatgt tcatccgcgc
     6301 atctcactta ctgaatcgat gttcaggttc tggcgacgac ggtgaagttg cagcagcagc
     6361 gcaaggccga tactcgcttc tgccgccgcg aggctgatgg cgagaatgta catcacctga
     6421 ccgtcggtct ggccccagta acttccggcg accacaaagg ccagcgcgga agcgttaatc
     6481 atgatttcca gaccaatcag cataaacagc agattgcgac ggataaccag accggttaag
     6541 ccaagaacga ataagattgc cgcgaggatc agtccatgtt gtaaggggat catgcgtgct
     6601 cctccgtttt tcttttcgcg ctgtcgtctt tacgattgct cagcacttca cccgcacgct
     6661 cttcacgacc gacgtggaag gccacaacca gacccgcgag cagcagcata gaagccagtt
     6721 ccaccgccag tacgtaaggc ccgaacagcg taatacccac tgctttagca ctgattggcg
     6781 taccgtcgat accttgatcg ttaacaccga gaatggcgta aacaatcacc accaacatga
     6841 tggctgacaa aattgccgga ccaatccaca cctgcggttt cagccactgg cgttcttgtt
     6901 cgatttctga accgcccagg ttgagcatca tcaccacgaa cacgaacagc accataatgg
     6961 cacccgcgta gacgataatt tccagcgcac cggcgaagta agcgcctagt gagaagaaca
     7021 ccccggaaat cgccagcagc gaaataatca ggtacagcag tgcgtgtacc ggattggtat
     7081 gggtgatcac tcgcaaggtc gcaagtatgg ctatcaggcc acagatataa aaagcgaact
     7141 ccattgcccc tctccttacg gtaacaggct cttgacgtcg ataggcttgg cttcgttctc
     7201 tgcttcgccc ttatctttgc cgtcgattgc catacctgcc atccggtaga agttatactc
     7261 cgggtatttg cccggaccgg agatcagcag atcctctttc tcgtaaacca gatcctggcg
     7321 cttgtattcc cccatttcga aatccggggt taactgaatc gccgtggtcg gacaggcttc
     7381 ttcgcacaaa ccacagaaaa tgcagcgtga gaagttgatg cggaaaaatt ccggatacca
     7441 gcgaccgtct ttggtttctg ctttttgcag cgagatacag ccgaccgggc aggctaccgc
     7501 gcagaggtta caggctacgc aacgctcttc gccgtccggg tcgcgggtca gaacgatacg
     7561 accacgataa cggggcggca gatagaccgg ctcttccggg tacattcgcg tttcgcgttt
     7621 ggcgaacgcg tgcaggccga tcatccagat actacgaacc tgggtgccga aacctactaa
     7681 caattctttt aaggtcatgg tcttattgcc ccttattgcg cctgccagag aatgacagcc
     7741 gccgttacca gcaagttgat cagcgtcagc ggcaggcaga ttttccagcc gaaggacatt
     7801 acctggtcat aacgcggacg cggtaacgac gcacgaatca aaatgaacat catcataaag
     7861 aacgcggttt tcagcgcgaa ccagatgaat ggcggtaaca acgggccttg ccagccaccg
     7921 aagaacagcg tcaccatcaa tgcagagatg gtcacaatcc cgatgtactc acccacgaag
     7981 aacagaccga acttcatacc ggaatattca atgtggtaac catccgccag ttcctgctcg
     8041 gcttccggct ggtcaaacgg gtgacggtga cacaccgcca cgcccgcgat ggcaaaggta
     8101 ataaaaccaa agaattgcgg gatgacgttc cacacatgcg cctggctgtt gacgatgtcg
     8161 gtcatgttga atgaaccggc ctgcgccacc acgcccatca gggaaagccc gaggaacact
     8221 tcgtagctca gggtctgcgc agaagcacgc atcgcaccga gcagcgagta tttgttgtta
     8281 cttgaccagc ccgcaaacag caccgcgtaa accgccagac cggccatcat caggaagaac
     8341 aaaatcccga tgttcaggtc ggcaaccacc caacccggac tgactggcac aatcgcaaag
     8401 gccagcagca gcgaggtaaa ggcaatcatc ggtgccaggg taaagatgac gcgatccgag
     8461 aatttcggga tccagtcttc tttaaagaac attttgatca tgtccgcaac cagctggagc
     8521 gaaccacccc agccaacacg gttaggtccg taacggttct ggaacagacc cagcaggcga
     8581 cgttcgccaa agctcatgaa tgccccgcag gtgacaacca ccagcaggat caccaccgct
     8641 ttgaggatgg tcagcaggat ctcaatcagt tccggtgata tccaactcat tgttgtgcct
     8701 ccttgagatc ctcaagatgc gcgccagcca gcaccggagc aatgccggac atacccatcg
     8761 gcaagcccac ctgccctgcc gtcagccctt cagcgatttc aaccggcaga gtgaccgtgt
     8821 tgccatcgta actaaaggag acgcgtgtac ctgcgtttac acccaacttc gcggcatccg
     8881 ctgggttgag tttgatgtac ggctgcggca tacgacactg gaagaccgga gcacgctgtg
     8941 acaattcatc gctgccaaac aggtggtagt acggcgcgat acgccatttc ccgtcctgcg
     9001 gctggaagcg tgccggtacg ctggtgaagt aatccagacc attttcgctg gtttcaaaca
     9061 gacgcacacc cggatcgcca aagcgcagtt tgccgcccac ttcgtcctgg aatttgttcc
     9121 acgcctgcgg ggagttccag cccggagccc aggcaaacgg cacttgcgaa cggtgcgcgg
     9181 tcggctggtt gttaccttcc atcgagaagg tgaacatggt gtcaatatcc tgcggctgac
     9241 gcggctcatg aacgctgata ttggcgcgca tggcggtacg accgctgtaa cggtgcggtt
     9301 cacgggccag tttctgccca cgaatacgga atgtcgcatc tggcgcagca tctttgatac
     9361 ccgccagttc cgggattttc gccacaacag cgtcaattac atggtcgagc tgcgtccagt
     9421 ccacttcacg gctcagcaag gtgctgtgca gcgagtgcag ccagcgccaa ctttccagca
     9481 tgacagtttt gctgtcgtaa taagccggat cgtaaacctg gaagaaacgt tgggcgcgac
     9541 cttcattatt aattaccgta ccgtcgcttt cagcaaagct ggcagcggaa agcaccagat
     9601 gggcgttttc catgatcgct gtgcgttgat ggtcaaccac catcaccagc ggcgctttag
     9661 ccagcgcagc attcacgcgg gtagcagaag cgtgacgatg cagatcgttt tccagcacca
     9721 ccaccgcgtc ggcgcgtccg gtttccagtt cggttaacgc ttcttcaagc gatccaccgc
     9781 ccataatgcc caaccccatg ctgttgacgg aacgggcaat catggtgata ccgacgtcag
     9841 cgccgcgacc tttcagggct ttcgcgacgt tagccgcagc ctgaatcacc tctaagctac
     9901 cggcgttcgt cccggagata atcaacggtt tcttcgcacc cgccagtgcc tgcacgatga
     9961 cgtcgatttt gctttgcagc tcaggttcaa taccgtcaac cgctggcgca gagttatcca
    10021 gcgcatgggc gatggcaaaa cctaaacgcg cctgatcttc aaccggtgcg cggtaagtcc
    10081 acgccgcgat atcatccaga cgggtgtcat caacgttggt aacaaacagc ggatgcttag
    10141 cacgttgacc gatattgagg attgccgcaa tctgccagtc agccactttc tgtgctgccg
    10201 ccatttcgcg cgctttacct ttcaccgcct ggcgcactgc cagcgcgacg cgcgcgccgg
    10261 tctgggtaac gtcttcgccc agcaccagta ccgcatcgta agattcgatt tcgcgcagag
    10321 ccggagtata aatgccgcct tcacgcagca ctttcagcgc cagttgcaga cgttcctgct
    10381 caccgtgagc gataccggtg tagaagtttt cttcgcccac cagttcacgc agcgcaaagt
    10441 tgctttccac gctggcacgc ggagaaccaa taccgatcac tttcttcgac tgacgcagaa
    10501 tatctgccgc gccctgcatt gcctgttcgg cgttgagggt aatgaaatca tcgccacgac
    10561 gctgtactgg ctgacgcgga cgatctttca ggttgacgta accgtaaccg aaacgaccac
    10621 ggtcgcagag gaagtagtgg tttaccgtac cgttgtaacg gttttcgata cgacgcagtt
    10681 cgccataacg ctcgccgggg ctgatgttac agccgatgga acattgctgg cagatgctcg
    10741 gcgcaaactg catatcccat ttacggttgt aacgctcgga gtgcgttttg tcggtaaata
    10801 cgccggtcgg gcaaatttcg accaggttac cggaaaattc gctttccagc gtgccgtctt
    10861 ccgggcgacc gaagtagacg ttgtcatgcg cgccataaac gcccagatct gtaccgtcag
    10921 cgtaatcttt gtagtaacgc acgcagcggt agcaggcgat gcagcggttc atttcgtgag
    10981 agatgaacgg ccccaaatcc tgattacggt gggtacgttt ggtgaaacgg tagcgacgga
    11041 agctgtgtcc ggtcatcacg gtcatatcct gaagatggca gttaccgcct tcttcacata
    11101 ccggacagtc gtgcgggtgg ttggtcatta accactcgac cacactttca cggaactgtt
    11161 tcgcttcttc gtcgtcaatg gaaataaagg tgccatcgga agccggtgtc atacaggaca
    11221 tcaccaggcg accacgcgtg tcttccgcgt tttggtattg cttcaccgca cactggcggc
    11281 aagcaccgac gcttcccagc gccggatgcc agcaaaagta aggaatatca aggcccagag
    11341 acagacaagc ttccagcagg ttgtccgctc cgttgacctc gtattctttg ccgtctacat
    11401 gaattgtagc cattagcatg cttccagttt tctcagtcag agactgagcg ttaatcgaaa
    11461 ttcggttacc agcgctcttt cagcaggttc ggctgaatcc cattaatcaa atgggtattg
    11521 ctgaacggct gtttgatccc cgcctcaaat tcttcgcgga aatatttgat ggcgctctgt
    11581 aacggctcca cagcaccagg tgcgtgggca cagaaagttt tacccgggcc taagaatcga
    11641 cacagttgct caagtgtttc gatatcgccc ggctgacctt cgccacgctc cagcgcacgc
    11701 agaattttca cgctccacgg cagaccgtcg cggcacggcg tacaccagcc gcaggactca
    11761 cgggcgaaaa actcttccag gttacgcacc agcgacacca tgttgatttc atggtcaacc
    11821 gccatcgcca gcgccgtacc cagacggctg cccgctttac cgatactttc gaattccatc
    11881 ggcaggtcaa ggtgcgcttc ggtcaggaag tcggtgcccg cgccgcctgg ctgccaggct
    11941 ttgaatttca gaccgtcacg cataccaccg gcgtaatctt cgaggatctc gcgtgcggtg
    12001 gtgccgaacg gcagttccca cagtcccgga tttttcaccc gaccggagaa gcccatcagc
    12061 ttggtgccag catctttact tttcgagatg ttctgatacc actccacgcc gttagcgagg
    12121 atcgccggaa cgttacacag ggtttcgacg ttgttgacac aggtcggttt gccccatacg
    12181 ccggaggttg ccgggaaggg gggtttcgag cgtgggttag cacgacgccc ttccagggag
    12241 ttgattaacg ctgtttcttc cccgcagatg tagcgccctg ccccggtatg gacgaacagt
    12301 tcgaagtcga aacctgttcc cataatgttt ttgccaagca atcccgcttc agtcgcttcg
    12361 gcaatggcgc ggcgcagatt tactgccgct tcaatatatt cgccacgcag gaagatgtag
    12421 ccacggtaag ctttcagcgc aaacgcggag atgagcatac cttccaccag caggtgcggc
    12481 agttgctcca tcaacaggcg gtctttatag gtgcccggct ccatttcatc ggcgttacac
    12541 agcaggtaac ggatgttcat ggattcgtct ttcggcatca ggctccactt caagccagtc
    12601 gaaaagcccg cgccgccgcg ccctttcaga ccagcgtctt ttacctgatt aacgatttcg
    12661 tccggagaca gcccggtcag cgccttacgc gccccttcgt aaccgttttt gctgcggtat
    12721 tcgtccagcc acactggctg tttgtcatcg cgcagacgcc aggtcagcgg atgcgtttcg
    12781 ggagtacgga taatgttttt catttatacc gctccagcag ttcagggatc gcttccgggg
    12841 tcagattcgc gtgagtgtcc tcatcgatca tcatgtttgg ccctttatca cagttcccca
    12901 ggcagcaagt tggcagcagc gtaaagcggc catcaaatgt cgtttgccct ggtttgatgt
    12961 tcagcttttt ctcgagcgcc gcctgaatac cctgataccc attgatatga cagaccacgc
    13021 tgtcgcaata acggatcaca tggcggccaa ccggctggcg gaagatctgg ctgtagaacg
    13081 ttgccacgcc ttcgacgtcg cttgccggaa tacccagcac atcggcgatc gcgtggatcg
    13141 caccatccgg cacccagcca cgctgcttct gaacgatttt cagcgcttca atggacgccg
    13201 cacgcgggtc ttcgtagtgg tgcatctcgt gctcgatcgc ttcacgctct gccgcactca
    13261 gctcaaaagc ctcggtttgt ggttgttgat tctcgtgcat aattagcggt ccacatctga
    13321 cataacaaaa tcgatactgc ccagataaac aatcaggtca gacaccaggc tgccgcggat
    13381 cgccgccgga atttgctgca aatgcgcata actcggcgtg cggatacgag tgcgataact
    13441 catggtgcta ccgtcgctgg tcaggtagta actgttgatc cctttggtcg cctcaatcat
    13501 ctggaaggat tcattggcag gcatcaccgg cccccacgac acttgcagga agtgggtgat
    13561 cagggtttcg atatgttgca gcgtgcgctc tttcggcggc ggcgtggtca gcgggtgatc
    13621 cgctttgaac gggccttccg gcatgttgtt gaggcactgc tcaagaatgc gcagactctg
    13681 gcgcagctct tccactttaa gcattacgcg ggtgtagcag tcagaaacac caccaccaac
    13741 cgggatttca aagtcgaagt tttcatagcc agaataagga cgcgccttac gcacgtcgaa
    13801 gtcgatcccg gtagcacgca ggcccgcgcc agtggtgccc cactccagcg cctctttcgc
    13861 gccataggcg gcaacgccct gggaacgacc tttcagaatg gtattttgca gcgccgcttt
    13921 ctcgtaagac gccagacgtt tcggcatcca gtcgaggaac tcacgcagca ggcgatccca
    13981 gccgcgcggc aggtcgtgcg ctacgccgcc aatacggaac cacgccgggt gcatacggaa
    14041 acccgtgatc gcttccacca gatcgtaaat tttctgacga tcggtaaagg cgaagaacac
    14101 gggcgtcatt gcgccgacgt cctgaataaa ggtcgagata tacagcaggt ggctgttgat
    14161 gcggaacagt tcggagagca ttacgcgaat gacgttaacg cgagccggca cggtgatccc
    14221 ggccagtttc tctaccgcca gcacgtaagg catttcgtta acgcagccgc cgaggtattc
    14281 gatacggtcg gtatacggaa tgtagctgtg ccaggactgg cgttcgccca ttttctccgc
    14341 accacggtgg tggtagccga tgtctggtac gcagtcgaca atctcttcgc cgtcgagctg
    14401 caaaacgata cggaaagcac cgtgcgccga cgggtggttc ggaccgaggt tgaggaacat
    14461 gaagtcctcg ttttcggtgc cgcgcttcat cccccactct tccggtttga aggtcagggc
    14521 ttccatctcc agatcctgtt tggctttggt cagctcaaac ggcgagaatt cggtagcgcg
    14581 cgccgggtaa tctttacgca gcgggtgacc tttccaggtt tgcggcatca tgatgcggcg
    14641 caggttcggg tgaccgtcga atgtaatgcc aaacagatcc caggtttcac gctcatacca
    14701 gttagcgttc gggaacagtt tggtgaaggt cggtacgtgc aggtcgtttt ctgccagcgc
    14761 caccttcagc atgatgtcgc ggttacgatc gatagaaatc agatggtaga aaacggaaaa
    14821 atccgcggca ggtaaccctt cgcggtgcgt gcgcagacgt tcgtccatgc cgtgtaagtc
    14881 aaacagcatg acgtaaggtt tcggcagttt ctttaagaaa tcgccaactt ccagtaattg
    14941 ttcacgcttg atccacacaa cgggaacccc ggtgcgagtc gcctgaacag taaaggcatc
    15001 cggcccaaaa cggttgcgca gttcgccaat taccggatca tcaagatgat cgcgggtctg
    15061 ccaggcgggt tcttgcgcgg ttaagtcggt catattgttc accattgcaa atggtccgtg
    15121 gtgactgtcg gcgcagcaag cttcgcgaaa tagatttagg aataagcgaa gaaaaatccc
    15181 tttgccgaca ggcgcaaatt aaatctcgtc aggtgtacgc aggttggtta cggcaatgcg
    15241 ttcaccgcgc ttgcgttcgc gctctgattg catattggcg cgataaacgc cctgatcgcc
    15301 aaccacccag gagagcggac gacgttcttt gccgatagat tcctgcaaca gcatcagtgc
    15361 ctgcatgtac gcttcaggac gcggcgggca gcccgggata tacacatcaa ccgggatgaa
    15421 tttatcgacg ccctgcacaa cggaataaat atcgtacata ccaccagagt tggcacaggc
    15481 acccattgag ataacccatt ttggttccag catctggtca tacagacgct gaataaccgg
    15541 tgccattttg gtaaagcagg ttcctgcaac caccatcagg tcagcctgac gcggcgaagc
    15601 acgcaatact tctgcgccaa aacgcgccac gtcatgcacc gcggtaaacg aagtcaccat
    15661 ctcaacgtaa cagcaggaaa gaccgaagtt atacggccaa attgagtttt tacgacccca
    15721 gttaaccatg tcattgagct tgcccataaa cacgtttttg ttaacttctt gctccagagg
    15781 gtcggttacg atctcctgct tttgcagggg gtaacggtcg ttctcaccgt tgggatctat
    15841 gcgggtgagc gtataatcca tcttaatgcc tcgcggttag cgttgacgat tagcgatact
    15901 gttcgtttcc gggttcatac gctcgcggcg tgaacgcgcg ggcgtccagt ccagcgcgcc
    15961 aatacgcacc agataaacca gacctgccag taacacaaaa ataaaaattg cagcttccac
    16021 aaagcctacc cagccgcttt cgcggataga ggttgaccat gcgaacagat acagcgcttc
    16081 aacgtcgaag ataacgaaga acatggccac cagataaaac ttggcggaca ggcgtaagcg
    16141 ggcggagccg accgagtcga taccggattc aaacggcacg tttttcgacc tcgcgcgtgc
    16201 gcgaccgcct aaaaaccaac cgcctaccag catcaggcaa cataggccaa tggcaacgat
    16261 aagaaagata gcgaatgccc agtgatgagc gatgacttca gtggatgttg acatactcat
    16321 tgcttactca tcaaaagtag cgccagattc actgctcttc acggcagatg gacgccacat
    16381 cgattcatgg ggaggaataa aaaaaacctt acaatcactg tagaaattct tttatacagc
    16441 taattgatgt ggtcttttac tcctttctat aaccttttgt caactttaac aaaagtttct
    16501 tcacattagt ttacataata tcaacaccat tagcatttaa tgccctttca ccccagatcc
    16561 ttgacgactc caggataatt agatgttgtt gaatcgtgtc cgttgtgaag caatggaaaa
    16621 aatacgggtc tattttgaca ggaatttgtg tcgattcctc cccccaaaag agagtatttt
    16681 cttgatctgt gacacgcttt tgtcattcca taacaaaaac gcagcaacaa atttacgtat
    16741 tttttaacat cattgtagca ggtgattttt ttcaggcgat tatttgtgcg tacggggcgt
    16801 gaatctctgg tgattgaaaa atgaacagtt ttgtacgttc tgcacgatgt gaagaaggtt
    16861 ctttactaaa aaaaagccgc tggggtttaa aacaccccca gcggctcgtt ttttacacta
    16921 ttgtctcagg aattatctat cgtccgtcga ttactcgata tccctttcaa tcaacaatga
    16981 atcatcccct tccggagcag atattgggct gtattgccac ggattatggt agctctccat
    17041 cgcctgatag atcacctggg ccagctcatt attactggac gggtcatagc acagcaaata
    17101 ttcggtatca ggtagcggcg gtaagccatc tactccactc agcacacgca agtccggact
    17161 catcatctcg accggccttg ccgtcacgcc aagaccggct ttcactgccg cacgaacggc
    17221 cggaagcgtc gaggcgacat aagccagtcg ccagggaata tctgctttat taagcgtcgc
    17281 cagcaccata tcgcgaaacg gacttggatc atccagcaat acaagaggga tcggctcacc
    17341 tttttgcaat acgtattccg ctgcgcagta ccagtgtgtt ggcgaggtac gcaggttcaa
    17401 tgccttaaac gctgaaggac gatgggtggt taccatcaaa tccacttcct gggattccag
    17461 catttctgcc atataggcat tacgtttaac gcggacatcc agcgctaatt tcggataaac
    17521 cgaactcacg cgatttaaca ggaaaggtaa gatcgtatcg gcagattcat ctgaagcacc
    17581 gatagttaac acgccctgaa gattactgaa cattaatgat gagcaggcct catcattaaa
    17641 acgcaggatt ttcctggcgt aaccaagaag ttgaatgcca tgttcagtta acagtttgtt
    17701 gcgaccgtga cgagcgaaca gttctttccc aacgagttgt tccagacgct gcatttgctg
    17761 acttacggcg gactgagtac gacacacagc ggcagctgcg gcagcaaaag tgttcagatc
    17821 ggcaacagca acaaatgttc tcagcagatc gaggtcgagg ttaattatcg gacgatttgc
    17881 acttatcata tattatcact tactggcggc tcatactgag ctggttaatg ctgtgcacac
    17941 acaaacaagc aattccattt gtaatgtgcc tccctggcag tttcatccgg aacccggacg
    18001 aaagtaaaaa tgcatatgag ttgcactaaa aaagcgactc acattgttcc gttataatgc
    18061 ctgaagtaga tcacagaata tatcttcagg gatcgcatat ctattaagtt actcactctt
    18121 ttctatttat gacatgcgcg tgtttgtata aatgtaaatg tgagtccttg ttccactctc
    18181 gtgcagcatc gctggtcata cgcgaacacg tacaaacagc aatggtgagg cggcatcaag
    18241 agcagggatc cgtcatttat gcgagcattt taccccaaaa accttttatt tataagggtc
    18301 attgcgaatt atctgatgca aagttatgtt atgttaggca aagtaatctt ctattattaa
    18361 taagcacatc aaaacctttt tgaatattaa ataataatta attagcatca tcctcattca
    18421 ttaattccgc ttaacaatag tttc
//
LOCUS       DBFIFI010000077        18318 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.077, whole genome shotgun sequence.
ACCESSION   DBFIFI010000077 DBFIFI010000000
VERSION     DBFIFI010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18318
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.077"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..536
                     /locus_tag="N7B39_002543"
     CDS             <1..536
                     /locus_tag="N7B39_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2422506.1"
                     /translation="QQAQLKSQLETIQKQQTGIISSTKTLTHVIKSVKDQQNTFIFTE
                     FNPAKTKYFILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGE
                     KPVYAKEFARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENI
                     QFDNLPSAENRQKRSKK"
     gene            580..5346
                     /gene="ehaG"
                     /locus_tag="N7B39_002544"
     CDS             580..5346
                     /gene="ehaG"
                     /locus_tag="N7B39_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric autotransporter adhesin EhaG"
                     /protein_id="HGU2422507.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIAIGAETEAAENATAIGNNAKAKGTNSMAMGFGSLADKVNTIALGNGSQALADNAIA
                     IGQGNKADGVDAIALGNGSQSRGLNTIALGTASNATGDKSLALGSNSSANGINSVALG
                     ADSIADLDNTVSVGNSSLKRKIVNVKNGAIKSDSYDAINGSQLYAISDSVAKRLGGGA
                     AVDVDDGTVTAPTYNLKNGSKNNVGAALAVLDENTLQWDQTKGKYSAAHGTSSPTASV
                     ITDVADGTISASSKDAVNGSQLKATNDDVEANTANIATNTSNIATNTANIATNTTNIT
                     NLTDSVGDLQADALLWNETKKAFSAAHGQDTTSKITNVKDADLTADSTDAVNGSQLKT
                     TNDAVATNTTNIANNTSNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGTET
                     TSKITNVKDGDLTTGSTDAVNGSQLKTTNDAVATNTTNIATNTTNISNLTETVTNLGE
                     DALKWDKDNGVFTAAHGNNTASKITNILDGTVTATSSDAINGSQLYDLSSNIATYFGG
                     NASVNTDGVFTGPTYKIGETNYYNVGDALAAINSSFSTSLGDALLWDATAGKFSAKHG
                     TNGDASVITDVADGEISDSSSDAVNGSQLHGVSSYVVDALGGGAEVNADGTITAPTYT
                     IANADYDNVGDALNAIDTTLDDALLWDADAGENGAFSAAHGKDKTASVITNVANGAIS
                     AASSDAINGSQLYTTNKYIADALGGDAEVNADGTITAPTYTIANAEYNNVGDALDALD
                     DNALLWDETANGGAGAYNASHDGKASIITNVANGSISEDSTDAVNGSQLNATNMMIEQ
                     NTQIINQLAGNTDATYIQENGAGINYVRTNDDGLAFNDASAQGVGATAIGYNSVAKGD
                     SSVAIGQGSYSDVDTGIALGSSSVSSRVIAKGSRDTSITENGVVIGYDTTDGELLGAL
                     SIGDDGKYRQIINVADGSEAHDAVTVRQLQNAIGAVATTPTKYFHANSTEEDSLAVGT
                     DSLAMGAKTIVNGDKGIGIGYGAYVDANALNGIAIGSNAQVIHVNSIAIGNGSTTTRG
                     AQTNYTAYNMDAPQNSVGEFSVGSADGQRQITNVAAGSADTDAVNVGQLKVTDAQVSQ
                     NTQSITNLDNRVTNLDSRVTNIENGIGDIVTTGSTKYFKTNTDGVDASAQGKDSVAIG
                     SGSIAAADNSVALGTGSVATEENTISVGSSTNQRRITNVAAGKNATDAVNVAQLKSSE
                     AGGVRYDTKADGSIDYSNITLGGGNGGTTRISNVSAGVNNNDVVNYAQLKQSVQETKQ
                     YTDQRMVEMDNKLSKTESKLSGGIASAMAMTGLPQAYTPGASMASIGGGTYNGESAVA
                     LGVSMVSANGRWVYKLQGSTNSQGEYSAALGAGIQW"
     gene            5714..7369
                     /gene="lldP"
                     /locus_tag="N7B39_002545"
     CDS             5714..7369
                     /gene="lldP"
                     /locus_tag="N7B39_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HGU2422508.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7369..8145
                     /gene="lldR"
                     /locus_tag="N7B39_002546"
     CDS             7369..8145
                     /gene="lldR"
                     /locus_tag="N7B39_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HGU2422509.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTIRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8142..9332
                     /gene="lldD"
                     /locus_tag="N7B39_002547"
     CDS             8142..9332
                     /gene="lldD"
                     /locus_tag="N7B39_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HGU2422510.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9518..9991
                     /gene="trmL"
                     /locus_tag="N7B39_002548"
     CDS             9518..9991
                     /gene="trmL"
                     /locus_tag="N7B39_002548"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HGU2422511.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDFRAFLEAENPQRLFALTTKGTPAHSAVNYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10044..10865)
                     /gene="cysE"
                     /locus_tag="N7B39_002549"
     CDS             complement(10044..10865)
                     /gene="cysE"
                     /locus_tag="N7B39_002549"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HGU2422512.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(10945..11964)
                     /gene="gpsA"
                     /locus_tag="N7B39_002550"
     CDS             complement(10945..11964)
                     /gene="gpsA"
                     /locus_tag="N7B39_002550"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HGU2422513.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(11964..12431)
                     /gene="secB"
                     /locus_tag="N7B39_002551"
     CDS             complement(11964..12431)
                     /gene="secB"
                     /locus_tag="N7B39_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HGU2422514.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12494..12745)
                     /gene="grxC"
                     /locus_tag="N7B39_002552"
     CDS             complement(12494..12745)
                     /gene="grxC"
                     /locus_tag="N7B39_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HGU2422515.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(12887..13318)
                     /locus_tag="N7B39_002553"
     CDS             complement(12887..13318)
                     /locus_tag="N7B39_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HGU2422516.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13563..15107
                     /gene="gpmM"
                     /locus_tag="N7B39_002554"
                     /gene_synonym="pgmI"
     CDS             13563..15107
                     /gene="gpmM"
                     /locus_tag="N7B39_002554"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HGU2422517.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15141..16400
                     /gene="envC"
                     /locus_tag="N7B39_002555"
     CDS             15141..16400
                     /gene="envC"
                     /locus_tag="N7B39_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HGU2422518.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16404..17363
                     /locus_tag="N7B39_002556"
     CDS             16404..17363
                     /locus_tag="N7B39_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HGU2422519.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17350..18318)
                     /gene="waaH"
                     /locus_tag="N7B39_002557"
                     /pseudo
     CDS             complement(17350..18318)
                     /gene="waaH"
                     /locus_tag="N7B39_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
BASE COUNT         4572 a         4751 c         4782 g         4213 t
ORIGIN      
        1 aacaacaagc acagctaaag agtcaactgg aaactattca aaaacaacaa actggcataa
       61 taagcagtac aaagacttta actcacgtaa ttaaatccgt taaagatcaa caaaacactt
      121 ttatttttac cgagtttaac ccagcaaaaa cgaagtattt catcctgaac aatggttcgg
      181 tggctttagc gggtcgagtt ttatccattg acgccacaga gaatggcagt gttattcata
      241 tttcactggt caacttatta agtacaccta tctcaaatat tggttttaat gcgacatggg
      301 gtggtgaaaa acctgtctat gccaaagagt ttgcccggtg gcaacaatta cttttcaaca
      361 catcaatgaa atccacattg aaattattac caggtcaatg gcaagacatt aatttgaccc
      421 tgaagggtgt atcgcccaat aacctgggat atctgaagtt agccatcaac atggaaaata
      481 ttcagttcga caatcttccc tctgctgaaa atcggcagaa aagaagcaaa aaataaatgc
      541 agttagtacg cttataacaa ggaatagcga gcatcattaa tgaacaaaat atttaaagtt
      601 atctggaacc ctgcgacagg gaattatact gttaccagcg aaacggcaaa aagccgtggc
      661 aagaaatctg ggcgcagtaa gctgttaatt tctgcgctgg ttgcgggtgg aatgttgtcg
      721 tcgtttgggg cattggcgaa tgccgggaat gacaacggtc agggtgttga ttacggtagt
      781 ggatcagctg gcgacggctg ggttgctata ggcaaagggg cgaaagcaaa tacttttatg
      841 aacaccagtg gttccagtac tgctgtgggt tatgacgcta tagctgaagg ccaatatagc
      901 tctgccatcg ggtcaaaaac ccatgcgatt ggtggtgcat caatggcctt tggggttagt
      961 gcaatatcag aaggcgatag aagtatagca ctgggtgcct cttcgtattc attgggccaa
     1021 tactcaatgg ccctcggccg ttattcaaaa gcattgggta aattgtctat tgctatgggg
     1081 gactcttcca aagcggaagg agcaaacgcc attgccctgg gaaatgccac taaagctact
     1141 gagattatga gtattgctct tggcgacacc gccaatgcgt caaaagcgta ttcaatggcg
     1201 ctgggagcaa gtagcgtcgc atctgaagaa aacgctattg cgataggtgc tgagaccgaa
     1261 gccgctgaaa atgcaactgc tattggcaat aatgcgaagg caaaagggac taatagcatg
     1321 gcaatggggt tcggaagcct tgccgataaa gtcaatacta tcgcattagg aaatggcagc
     1381 caggctctgg cagataatgc aatcgccata ggccagggca acaaagctga tggcgtggat
     1441 gccatcgctc tgggtaatgg tagccagtcg agaggcttaa acaccattgc cttaggcaca
     1501 gccagtaatg caactggtga taagagtctt gcgcttggta gtaatagcag tgccaacggt
     1561 attaactctg tcgcgctggg cgcagattcc attgcggatt tagacaatac cgtctctgtc
     1621 ggcaatagtt cattaaaacg caagatcgtt aatgtgaaaa atggcgcgat caagtctgac
     1681 agttacgatg ccattaatgg ttcacagctt tatgccatta gcgactcggt agcaaaaagg
     1741 cttggaggag gggctgcagt agatgttgat gacggtactg ttacagcacc aacctacaat
     1801 ttaaaaaatg gtagcaaaaa taacgtaggg gctgcgctcg ctgtacttga tgaaaacacc
     1861 ctgcaatggg accaaaccaa aggcaaatac agcgctgctc atggtactag tagcccaact
     1921 gccagcgtaa tcaccgatgt tgcggatggc acgatttcag cctccagtaa ggatgcggtt
     1981 aacggttccc aactgaaagc taccaatgac gatgtcgaag ccaacaccgc caatatcgct
     2041 actaatacca gcaacattgc cacgaatacg gcaaatattg ccaccaatac caccaatatc
     2101 accaacctga cggattccgt tggtgacctt caggctgatg ccctgctctg gaacgaaact
     2161 aaaaaggcat tcagtgcagc tcacggccag gataccacca gcaaaatcac caacgttaaa
     2221 gatgccgacc tgacggctga cagcactgat gctgttaacg gctctcagct gaaaaccacc
     2281 aacgatgctg tggcgacgaa taccaccaat atcgccaata acacttccaa tattgccact
     2341 aacaccacca acatctctaa cctgactgag acggtgacta atcttggtga ggatgcgctg
     2401 aaatgggata aggacaatgg tgtattcacg gcagctcatg gcaccgagac caccagcaaa
     2461 atcaccaacg ttaaagatgg cgacctgacg actggcagca ccgatgccgt taacggctct
     2521 cagctgaaaa ccaccaacga tgccgtggcg acgaatacca ccaatatcgc cactaacacc
     2581 accaacatct ctaatctgac tgagacggtg actaatcttg gtgaggatgc gctgaaatgg
     2641 gataaggaca atggtgtctt cactgcagct catggcaaca ataccgccag caaaatcacc
     2701 aatatcctgg acggcacagt cactgcaacc agttccgatg ccattaacgg tagccagctt
     2761 tatgacttaa gcagcaatat cgccacctac ttcggcggca atgcttctgt gaatactgac
     2821 ggtgtgttta ccggtccaac ctacaaaatc ggtgaaacaa attattataa cgtcggcgat
     2881 gcactggctg cgattaactc ctcatttagc acgtctctcg gcgatgctct gctttgggat
     2941 gccaccgcag gtaaattcag tgccaaacac ggtactaatg gtgacgcaag cgtgatcact
     3001 gatgtcgcag atggtgaaat ttcagactcc agttctgacg cagtaaacgg ctcacaactc
     3061 cacggcgtga gcagttatgt tgttgatgcg ctggggggtg gtgccgaagt caatgcagac
     3121 ggcaccatca ctgcgccgac gtacaccatt gctaatgctg attacgataa tgtcggtgat
     3181 gccctgaatg ctatcgatac cactcttgac gacgctctgc tctgggatgc ggacgccggt
     3241 gaaaatggtg catttagcgc cgctcacgga aaagataaaa ctgccagtgt aatcactaac
     3301 gtcgctaacg gtgcaatctc tgctgccagc agcgacgcga ttaacggctc acaactctat
     3361 accaccaata agtacatcgc tgatgcgctg ggtggtgacg cagaagtcaa cgctgacggc
     3421 accatcaccg caccgactta caccattgcg aacgccgagt acaacaacgt cggtgacgcc
     3481 ctggatgcgc ttgatgataa cgccctgctg tgggatgaga ctgccaatgg cggtgctgga
     3541 gcctacaatg ccagccatga cggtaaagcc agcatcatca ctaatgtcgc taatggcagt
     3601 attagtgagg acagtaccga tgcagtgaac ggttctcagt tgaatgcgac gaatatgatg
     3661 attgagcaga acacccaaat tatcaatcag ctcgctggta acaccgacgc aacctatatc
     3721 caagaaaacg gtgcgggtat taactatgtg cgtactaacg acgacggctt agcgttcaac
     3781 gacgccagcg cacagggtgt tggcgctaca gctataggtt ataactctgt cgccaaaggc
     3841 gatagcagcg tagctattgg tcagggcagc tacagcgacg ttgatacggg tatcgccctg
     3901 ggtagcagct ctgtttccag ccgagtgatt gccaaaggct cccgtgacac cagcataacg
     3961 gaaaatggcg ttgttattgg ttacgacacc acggatggcg aactgctcgg tgcattgtct
     4021 atcggtgatg acggtaaata tcgtcaaatc atcaacgtag ccgatggttc cgaagcccat
     4081 gacgccgtta cggttcgtca attgcagaat gcgattggtg cggtcgcaac cacgccgact
     4141 aaatacttcc acgctaattc aacggaagaa gattcactgg cagtgggaac tgactcgctg
     4201 gcaatgggtg cgaaaaccat cgtgaatggc gataaaggta ttggtatcgg ttatggtgcc
     4261 tacgtggacg cgaatgcact taacggcatt gccattggta gcaatgcgca agtcattcat
     4321 gtcaacagta ttgcgatagg taatggttct acgaccactc gtggcgctca aaccaattat
     4381 accgcctaca acatggacgc accgcagaac tctgtcggtg aattctcagt cggtagtgcg
     4441 gatggtcaac gtcagatcac taacgtcgca gcaggttcgg ctgataccga tgcggtcaac
     4501 gtgggtcagt tgaaagtaac ggatgcgcag gtttcccaga atacccagag cattactaac
     4561 ctggataatc gggtaacgaa tcttgattca cgcgtcacca atatcgaaaa cggtattggc
     4621 gatatcgtca ccaccggtag caccaagtac ttcaagacca ataccgatgg tgtagatgcc
     4681 agcgcgcagg gtaaagatag cgtcgcgatt ggttccggct ccattgctgc cgctgacaac
     4741 agcgtcgctc tgggtacagg gtctgtggca accgaagaaa atacgatctc tgtaggttcc
     4801 tctactaacc aacgtcgtat caccaacgta gctgcaggta aaaatgctac cgatgctgtt
     4861 aacgtggcac agttgaagtc ttccgaagct ggcggtgtac gttacgacac caaagctgat
     4921 ggttctatcg actatagcaa tatcaccctc ggtggcggca acggcggtac gactcgtatc
     4981 agcaacgtct ccgctggcgt caacaacaac gacgtggtga attacgcgca gttgaagcaa
     5041 agcgtgcagg aaacgaagca atacaccgat cagcgaatgg ttgagatgga taacaaactg
     5101 tctaaaactg aaagcaagtt gagcggtggt atcgcttctg caatggcaat gaccggtctg
     5161 ccgcaggctt acactccagg tgccagcatg gcctctattg gtggcggtac ttacaacggt
     5221 gaatcggcag ttgctttagg tgtatcgatg gtgagcgcca atggtcgttg ggtctacaaa
     5281 ttacaaggta gtaccaatag ccagggtgaa tactccgccg cactcggtgc cggtattcag
     5341 tggtaatgat ccattaagtt agtgtgacta agggcaaacg ccaaattgcc tgatgcgtta
     5401 cgcgcatcag gcaagataag gcgctccggg tcaacaatct aagggcaatt ctctgatgag
     5461 gattgccctt ttctttacca gacatctccc cccacaagaa ttggccctac caattcttcg
     5521 cttatctgac ctctggttca caattcccca attaaaactc acatcaatat tgccaataca
     5581 taacatttag ttaaccattc attgtcatta tccctacaca acacaattgg cagtgccact
     5641 tttacacaac gtgtgacaag gagatgagca acagactcat tacacgatgt gcgtggactc
     5701 caggagacct gcaatgaatc tctggcaaca aaactacgat cccgccggga atatctggct
     5761 ttccagtttg atagcatcgc ttcccatcct gtttttcttc tttgcgctga ttaagctcaa
     5821 actgaaagga tacgtcgccg cctcatggac ggtagcaatc gcccttgccg tggctttgct
     5881 gttctataaa atgccggtcg ctaacgcgct ggcctcggtg gtttatggct tcttctatgg
     5941 tttgtggccc atcgcgtgga tcattattgc agcagtgttc gtctataaga tctcggtgaa
     6001 aaccgggcag tttgacatca ttcgctcgtc tattctttcg ataacccctg accagcgtct
     6061 gcaaatgctg atcgtcggtt tctgtttcgg cgctttcctt gaaggagccg caggctttgg
     6121 cgcaccggta gcaattaccg ccgcattgct ggtcggcctg ggttttaaac cgctgtacgc
     6181 cgccggactg tgcctgattg ttaacaccgc gccagtggca tttggcgcga tgggcattcc
     6241 aatcctggtt gccggacaag taacaggcat cgacagcttt gagattggcc agatggtggg
     6301 gcggcagcta ccgtttatga ccattatcgt gctgttctgg atcatggcga ttatggacgg
     6361 ctggcgcggt atcaaggaga cgtggcctgc ggtcgtggtt gcgggcggct cgtttgccat
     6421 cgctcagtac cttagctcta acttcattgg gccggagctg ccggacatta tctcttcgct
     6481 ggtatcactg ctctgcctga cgctgttcct caaacgctgg cagccagtgc gcgtattccg
     6541 ttttggtgat ttgggggcgt cacaggttga tatgacgcta gcccacaccg gttacactgc
     6601 gggtcaggtg ttacgtgcct ggacaccgtt cctgttcctg acagctaccg taacactgtg
     6661 gagtatcccg ccgtttaaag ccctgttcgc atcgggtggc gcgctgtatg agtgggtgat
     6721 caacattccg gtgccgtacc tcgataaact ggttgcccgt atgccgccag tggtcagcga
     6781 ggctacagcc tatgccgccg tgtttaagtt tgactggttc tctgccaccg gcaccgccat
     6841 tctgtttgct gcactgctct cgattgtctg gctgaagatg aaaccgtctg acgctatcag
     6901 caccttcggc agcacgctga aagaactggc tctacccatc tactccatcg gcatggtgct
     6961 ggcgttcgcc tttatctcga actattccgg actgtcatca acactggcac tggcactggc
     7021 gcacaccggt catgcattca ccttcttctc gccgttcctc ggctggctgg gggtattcct
     7081 gaccgggtcg gatacctcat ctaacgccct gttcgccgcg ctgcaagcca ccgcagcaca
     7141 acaaattggc gtctctgatc tgttgctggt tgccgccaat accaccggtg gcgtcaccgg
     7201 taagatgatc tccccgcaat ctatcgctat cgcctgtgcg gcggtaggcc tggtgggcaa
     7261 agagtctgat ttgttccgct ttactgtcaa acacagcctg atcttcacct gtatggtggg
     7321 cgtgatcacc acgcttcagg cttatgtctt aacgtggatg attccttaat gattgtttta
     7381 cccagacgcc tgtcagacga ggttgccgat cgtgtgcggg cgctgattga tgaaaaaaac
     7441 ctggaagcgg gcatgaagtt gcccgctgag cgccaactgg cgatgcaact cggcgtatca
     7501 cgtaattcac tacgcgaggc gctggcaaaa ctggtgagcg aaggcgtgct gctcagtcga
     7561 cgcggcggcg ggacgtttat tcgctggcgt catgacacat ggtcggagca aaacatcgtc
     7621 cagccgctaa aaacgctgat ggccgatgat ccggattaca gtttcgatat tctggaagcc
     7681 cgctacgcca ttgaagccag caccgcatgg catgcggcaa tgcgcgccac acctggcgac
     7741 aaagaaaaga ttcagctttg ctttgaagca acgctaagtg aagacccgga tatcgcctca
     7801 caagcggacg ttcgttttca tctggcgatt gccgaagcct cacataacat cgtgctgctg
     7861 caaaccatac gcggtttctt cgatgtcctg caatcctcag tgaagcatag ccgtcagcgg
     7921 atgtatctgg tgccaccggt tttttcacaa ctgaccgaac aacatcaggc tgtcattgac
     7981 gccatttttg ctggtgatgc cgacggggcg cgtaaagcaa tgatggcgca ccttagtttt
     8041 gttcacacca ccatgaaacg attcgatgaa gatcaggctc gccacgcacg gattacccgc
     8101 ctgcccggtg agcataatga gcattcgagg gagaaaaacg catgattatt tccgcagcca
     8161 gcgattatcg cgccgcagcg caacgcattc tgccgccgtt cctgttccac tatatggatg
     8221 gtggtgcata ttctgaatac acgctgcgcc gcaacgtgga agatttgtca gaagtggcac
     8281 tgcgccagcg tattctgaaa aacatgtctg acttaagcct ggaaacgacg ctgtttaatg
     8341 agaaattgtc gatgccggta gcactggcac cggtgggttt gtgtggcatg tatgcgcgtc
     8401 gtggcgaagt tcaggcagcc aaagcggcgg acgcgcatgg cattccgttt actctctcaa
     8461 cggtttccgt ttgcccgatt gaagaagtcg cgccagccat caagcgccca atgtggttcc
     8521 agctttatgt actgcgcgat cgcggcttta tgcgtaacgc gctggagcga gcaaaagcag
     8581 cgggttgttc gacgctggtt ttcaccgtgg atatgccgac accgggagcg cgttatcgtg
     8641 atgcgcattc tgggatgagc ggcccaaatg cggcaatgcg ccgctacttg caagcggtga
     8701 cgcatccgca atgggcgtgg gatgtgggcc tgaacggtcg tccgcatgat ttaggtaata
     8761 tttcggctta cctcggcaaa ccgaccggac tggaagatta catcggctgg ctggggaata
     8821 acttcgatcc gtccatctca tggaaagacc ttgagtggat ccgcgatttc tgggatggcc
     8881 cgatggtgat caaagggatc ctcgatccgg aagatgcgcg cgatgcagta cgttttggtg
     8941 ctgatgggat tgtagtttct aaccacggtg gccgccagct ggacggtgta ctctcttccg
     9001 ctcgtgcgtt gcccgctatt gcagatgcgg tgaaaggtga tatcgccatt ctggcggata
     9061 gcggaattcg taacgggctt gatgtcgtgc gtatgattgc gctcggtgcc gacaccgtac
     9121 tgctgggtcg tgctttcctg tatgcgctgg caacagcggg ccaggcgggt gtagctaacc
     9181 tgctaaatct gatcgagaaa gagatgaaag tggcaatgac gctgactggc gcgaaatcga
     9241 tcagtgaaat tacgcaagat tcgctggtgc aggggctggg taaagagttg cctacggcac
     9301 tggctccgat ggcgaaaggg aatgcggcat agtcgtttga gccccctcac cctaaccctc
     9361 tccctcaggg agtggggacc gttcggcgct gtatgtactc cctcactctg aaacgacacc
     9421 gcactctttt tttctccctc gcccctccgg ggtgagggga aaaggccgca ctgctcacca
     9481 attttctgct atcctgcccc cgcactaagg gggcagtatg ctaaacatcg tactttacga
     9541 accagaaatt ccgccaaata ctggcaatat catccgtctt tgcgctaata ccggctttcg
     9601 tctgcatatc atcgaaccga tgggatttgc ctgggacgat aagcgcctgc gccgcgcggg
     9661 gctggactat cacgagttta ccgccgttac gcgtcatcat gactttcgcg cgttcctcga
     9721 agcagaaaat ccccagcgcc tgtttgccct caccacgaaa ggtacgcctg ctcacagcgc
     9781 cgtaaactat caggatggcg attatctgat gttcggcccg gaaacacgcg gcctgccagc
     9841 gagcattctt gatgccctgc ccgctgaaca aaaaattcgc attccgatgg tgccggacag
     9901 ccgcagcatg aacctgtcca atgcggtgtc agttgtggtg tatgaagcct ggcggcagct
     9961 gggatatccg ggagcgttgt tgagagatta gttactgtat gccggataga tgattacatc
    10021 gcatccggca cgatcacagg acattagatc ccatccccat actcaaatgt atggttaata
    10081 ccgttgaaat gctggtccat atccattgat ggcttatcgc tgtctggttt accgacaata
    10141 cgagccggaa cgccagcggc ggttgtatgc ggcggcaccg gttgcagcac cacggaacct
    10201 gcgccaatct tcgcgccgcg accaacttca atattgccga ggattttcgc gcccgcgcca
    10261 atcatcacac cttcacgaat tttcgggtga cggtcaccac cagatttacc cgtaccgcca
    10321 agcgtcacag attgcagaat cgatacgtcg ttttcaatta ccgccgtttc acccacaacg
    10381 atgcccgtgg cgtggtcgag cataatgcca cggccaattt ttgctgccgg gtgaatatcg
    10441 acctggaacg tcacagaaac ctggttttgc agaaagattg ccaatgcgcg acgcccctga
    10501 ttccacaacc agtgaccgat gcgataggcc tgcaaggcat gaaaaccctt caggtataac
    10561 aacggggttg agtatttatc gactgccggg tcgcgggtac gcaccgcctg aatatcacag
    10621 gccgcagagg cgatcatttc cgggtcagcg gcgtaggctt cttccaccac ttcacggata
    10681 gcaatagcag gcataattgg cgatgacagc ttgttcgcca gcatgtagct cagtgcacta
    10741 ccaaggtttt cgtgcttgag tagcgtcgcg tggtaaaaac tggccagcat tggctcacag
    10801 tccgccagcg ttctggcttc ggctttaata ttgttccaga caatttccag ttcttcacac
    10861 gacattgctt actccacacg atgagataat gaccggcccg ttctgcgcgg gccgggtcat
    10921 agcggtaaca aaggttccct ggggttagtg gctgctgcgc tcgtccttgc gtgcacgacc
    10981 taataacgtc aatgctgcct cgcgcgcgtt ttttccgcaa tataatactt gataaatttc
    11041 ctcggttatt ggcatttcta cgccgaagcg atgcgccagt tcgcggactt ctttcgtatt
    11101 gcggtagcct tccaccacct gaccaatctt ctcctgcgcg ctttgtacat ccatgccctg
    11161 accgagcatc atgccaaaac ggcggttacg cgactggttg tcggtacaag taagcacaag
    11221 atcgcccagc cccgccatgc ccataaaggt ggcaggatcg gcacccagcg ccgcgccaag
    11281 acgcgacatt tcagccagcc cacgggtgat cagtgccgta cgcgcattcg caccaaaacc
    11341 gataccgtcg gacatccccg cgccaatggc aatgacgttt ttcaccgcac cgccaagctg
    11401 cacgccaatg aaatccggat tgctgtaaac gcggaaactt ttgccgcagt gcagcagctg
    11461 ctggagatca tcggcaaagg tctgatcggt cgaggccagc gaaatagctg tcggtaaacc
    11521 tgccgccagt tctttcgcaa acgttgggcc agagataacc gccagcggaa tttgatcgcc
    11581 taaggcctca cgcgccacgt cctgtaacag acgtccggtt tccgcttcca gccctttggt
    11641 cgcccacacc agacgcgcat caggacgcat cagcggttta atctggcgca gcacttcacc
    11701 aaagacatgg ctgggtacga cgacgagaat attacggctg gctgccagcg cagtggcgag
    11761 atcgctttca agatggagcg tatcgggaaa aggcacatcg gggagaaacg cggcgttaca
    11821 gcggtcgcgt tcaagcgttg cgatatgttc agggtcatgg ccccagagga caacctcgtg
    11881 gccatttctt gccagggtga tggcaagagc ggtgccgtac gagccggcac cgatcacagt
    11941 cattgaagca ttacgttggt tcatcaggca tcctgatgtt cttcagtacc ttcgccagcc
    12001 tgctgctgca aatagttcat gaacagcgca tcgaagttaa ccggcgcaag gttcagttgc
    12061 gggaatgtac cgcgggatac catgctggtg atgcactcac gagcatacgg gaacagaatg
    12121 ttcgggcagt atgcgcccag gcaatgtgcc atctgggtgc cttcgatacc cgcgatggag
    12181 aaaataccgc cctgctgaac ttcacacagg aacgcggttt cttcgcccag ggaggccgtt
    12241 acggtaacac gcagtaccac ttcgtatacg tcatctgcca gttgggaaga tgccgtatcc
    12301 agatcaagtt taacttctgg ttgccaatct ttctggaaaa cgtgcggcgc gttcggcgct
    12361 tcgaaagaga tatccttggt ataaatacgt tggatctgga aagtcatttc agtgttgttt
    12421 tgttctgaca tgtgtagaaa acccttaagt gttgtcctta aatactgcgc aatgtaatgc
    12481 caacgttcac acgttatttc agcaggggat ccagtccacc acgtgcatcc aatgcataca
    12541 agtcatcaca gccgccaatg tgctgtgcgt caataaaaat ctggggaacc gtggtgcgac
    12601 cgctgcgttt gatcatctct tcacgcttgg cggcattgcc atcgatcggc aactcctgaa
    12661 aactcacgcc cttgctgctc agcagtgctt ttgcacgatg gcaatacggg caggtttctt
    12721 tggtatagat ttcaacattg gccataattt agctcctgtt tttttaccct gaaatttcat
    12781 gttgcagggc ggctgcaaaa tggagtgtcc ccagaagctt ataacaataa gtcgctgggg
    12841 gcctaattgc agctaacggc ctgcatcatg aaagacgaca ggtaaattat ttgccgcgca
    12901 ccaaaggcaa gttttcgcca gcccagccag cgacgccttc tttcagcacg aatacttgcg
    12961 caaaaccagc tttcgtcagt gcgtttgcag gctcctggca ctgcatgcca gaaccgtcta
    13021 ccacgataac cggtttgtct ttgtgcttct caagctcacc aacattgttg gctttgattt
    13081 cgctcggcaa cagattgata gaacctgcga tatggccttt acggaagtca tcacgctgac
    13141 gtaaatccac aaccacagcg tcttctttgt tgatcagacg cgtagcttca ccacgagtaa
    13201 tcaccttcac tttcgaggtc aggctcttaa acgtagtcac aagaaccgcc accagtaacg
    13261 cgatccaggc gatactcagt atgggatgac ggccaacaaa ttgcataatt tcttgcatgg
    13321 ggggtaacaa ctcccgacgt agtgattaaa aaaaccagga aaggagtata cctgcgcggt
    13381 gcggcaaata cagccagcgc gttaactgga atgcaatttt gcggggcacg acgaaaaaaa
    13441 agcgcaaatc tggcatcggc ttgccgcgag cggtcgtata ttttgatctt cagaggctat
    13501 tttatcgatt cagctgtagt aaaattacgc aaattttgac tcttgagtat gaggttgtcg
    13561 caatgtcggt ttctaaaaaa cctatggtac tggtgattct ggatggctat ggctatcgtg
    13621 aagaacagca ggataacgcc atttttagtg ctaaaacccc ggtaatggat gcactgtggg
    13681 ccaatcgtcc gcataccctg atcgacgctt ccggtctgga agtcggtctg cctgaccgtc
    13741 agatgggtaa ctccgaagta ggccacgtta acctgggtgc cggccgcatc gtgtatcagg
    13801 acctgactcg tctggacgtt gaaatcaaag atcgcgcttt ctttgctaat ccagtgctga
    13861 ctggcgcggt agataaagcg aaaaacgcag gtaaagcagt acacattatg ggtctgctct
    13921 ccgcaggcgg cgtacacagc cacgaagatc acatcatggc gatggtagaa ctggcagctg
    13981 aacgcggcgc agaaaaaatc tacctgcacg cattccttga cggtcgcgac accccgccgc
    14041 gcagtgctga atcctcgctg aaaaaatttg aagagaaatt tgctgcgctg ggtaaaggcc
    14101 gcgtagcgtc catcattggt cgttactacg cgatggaccg cgataaccgt tgggatcgcg
    14161 ttgaaaaagc ctatgacctg ctgactctgg cgcagggtga gttccaggcc gatactgccg
    14221 ttgctggttt gcaggcagct tatgctcgcg acgaaaacga cgagttcgtg aaagcgaccg
    14281 ttattcgtgc agaaggtcag ccagatgcgg caatggaaga cggcgatgcg ctgattttca
    14341 tgaacttccg tgctgaccgc gcgcgcgaaa tcactcgtgc attcgtaaac gcagatttcg
    14401 acggcttcgc gcgtaagaaa gtggttaacg tcgatttcgt gatgctgacc gaatacgccg
    14461 ctgacatcaa aactgcggtt gcttacccac ccgcttccct ggttaacacc ttcggcgagt
    14521 ggatggcgaa aaacgacaaa actcagttgc gtatttccga aaccgaaaaa tatgcccacg
    14581 ttactttctt cttcaacggt ggcgtagaag agtcgttcaa aggcgaagat cgcattctga
    14641 tcaactcacc gaaagtggct acctacgatc tgcaaccgga aatgagctcc gcagagctga
    14701 ccgaaaaact ggttgcggcc atcaagagcg gcaaatacga caccattatc tgtaactatc
    14761 cgaacggcga catggtaggt cacaccgggg tgatggaagc ggcggttaaa gcggttgaag
    14821 cactggatca ctgcgtggaa gaagtcgcga aagcggttga gtccgtgggt ggacaactgc
    14881 tgatcaccgc tgaccacggt aacgctgaac agatgcgcga tccggcaacg ggtcaggcac
    14941 acacggcaca caccaacctg ccagttccgc tgatttacgt tggtgataag aacgtgaaag
    15001 cggttgcagg cggcaaactt tctgacatcg cgccgaccat gttgtcgctg atgggtatgg
    15061 aaatcccgca agagatgact ggtaagccgc tgttcatcgt ggaataatcc ctccccatga
    15121 ggggaaaggc gattaatacc atgacacggg ccgtgaaacc gcgcaggttt gcaatcaggc
    15181 ccatcatcta cgccagcgtt cttagcgctg gcgtattgtt gtgcgccttt tccgcccacg
    15241 cggatgagcg tgaccaactc aaatctattc aggccgatat cgccgcaaaa gagcgcgcgg
    15301 tacgccaaaa gcaacaacaa cgcgcaagcc tgctcgcaca attgaaaaag caggaagaag
    15361 cgatctctga agccacccgt aagctgcgcg aaacgcaaaa cacgctcaat caactcaata
    15421 aacagattga tgagatgaac gcgtcgattg ccaaactgga gcagcaaaaa gccgcccagg
    15481 agcgcagcct cgccgcacaa ctggatgccg cattccgtca gggcgagcat accggtattc
    15541 agctgattct cagcggtgaa gaaagccagc gtggacagcg tttacaggct tatttcggct
    15601 atctcaacca ggcgcgacaa gaaaccattg cccagttgaa gcaaacgcgt gaagaagtcg
    15661 ccatgcagcg tgctgaactg gaagagaaac agagcgagca acaaacgctg ttatatgagc
    15721 agcgcgccca acaggcgaaa ctgactcagg cgctgaacga gcgtaaaaag acgctggcag
    15781 ggctggagtc ttccatccag caaggtcagc aacagttgag cgagctgcgc gccaacgaat
    15841 cccgtctgcg taacagcatt gcccgtgcgg aagccgcggc gaaagcgcgt gcagaacgag
    15901 aagcacgtga ggcccaggcg gttcgcgacc gccagaaaga agcgacgcgc aaaggcacca
    15961 cctacaaacc gaccgaaagc gaaaaatcgc tgatgtcccg aactggtggc ctgggggcgc
    16021 cgcgtggtca ggcattctgg ccggttcgcg ggccgacgct gcatcgctat ggtgaacagc
    16081 tacagggcga actacgctgg aaaggaatgg ttatcggtgc ctctgaaggt actgaagtta
    16141 aagcgattgc cgatggtcgg gtgattctgg ctgactggct gcaaggttac ggtctggtgg
    16201 tggtggttga gcacggtaaa ggcgacatga gtctttacgg ctataatcag agcgcactgg
    16261 tgagcgttgg ttcgcaggtt cgcgcgggcc agccaattgc actggtgggc agcagtggcg
    16321 gtcagggtcg gccttcactc tatttcgaaa ttcgccgcca aggtcaggcg gtcaatccac
    16381 agccgtggtt gggaagataa gttttgtttc catttcgtcg taacgttctt gcatttgccg
    16441 ctctgttggc gctctcctcc cccgtacttg ctggcaaact tgccatcgtc attgatgatt
    16501 ttgggtatcg cccgcacaac gaaaaccagg tgctggcgat gccctccgct atctccgtcg
    16561 ctgtattacc cgattcaccg cacgccagag agatggcgac caaagcgcat aacagcgggc
    16621 acgaagtgtt gattcatctc ccgatggcac cactgagtaa acagccgctg gagaaaaata
    16681 cgctgcgccc ggagatgagc agcgacgaaa ttgagcgcat tattcgtagt gcggtcaata
    16741 acgtgcccta tgccgtgggg atcaacaacc acatgggtag caagatgacc tctaacctgt
    16801 ttggtatgca gaaagtgatg caggcactgg agcgttacaa tctttacttc ctcgacagcg
    16861 taaccatcgg taatacccag gcgatgcgcg ccgcgcaagg tactggcgtg aaggtgatca
    16921 aacggaaggt gttcctcgac gattcgcaga acgaagcgga catccgtgtg caatttaatc
    16981 gcgcaattga tctggcgcgt cgcaacggtt caaccattgc cattgggcat cctcaccctt
    17041 caacggtacg cgtgctacaa cagatggttt ataacctgcc gccagacatt acgctggtga
    17101 aagccagcag cttgctgaat gaaccgcagg ttgatacttc tacaccgccg aaaaacgctg
    17161 tgcctgatgc accgcgtaat ccgttccgtg gcgtgaagct gtgcaaaccg aagaaaccga
    17221 tagaacctgt ttatgctaat cgcttctttg aagtattaag cgaaagcatc agccagagca
    17281 cgctgatcgt ttacttccag catcagtggc aaggctgggg caaacagccc gaagcggcga
    17341 agtttaacgc tagcgcaaat taaaggcgct acgcgtaatg cgatggtgcg accgcgtttt
    17401 tttcgcgcca ttgccataag cggaaagacc acagtaacgc ctggtaaccg accttcacgc
    17461 tgcgcacatt ggtaatcagg cgcttataca taccggaagt aaatatctcg gcaatcatcc
    17521 gttggcgggt aagaatatcc ggctctttgc gcaccgcatg gcaaacacgc aatgcttcgt
    17581 aagttatttg ctgatgaaat tccggataaa tcataatttt gtcggcataa tttcgattta
    17641 atttctccag caggcgggta atcttaatat agtgacgttg ataattaagg tttttattcc
    17701 cttgtctatg caaccgactc actgacgtat tatgcagata atatttatat aatgattgct
    17761 cggtatatcg cgcacgcagc gcgttaaaca tgaattctgt tgtccagaca atatcctgat
    17821 gatgtaatcc ggcaataaat ttaatgttat ttttaacaat aacatcacgg cgataaaccc
    17881 ccatccagac aacgtgagtc caacggcgcg aagaaagccc catccgcagc cagtccgggc
    17941 cggttaatac accggttgag cgaaggcgat cgctggggat ggattgccag gtttctcccg
    18001 tttcacgaaa agaccagtca gcgttgcact gcgccacgtc gaggtcgtcc tctaacgcca
    18061 tggtcatcag cgtttcgtac atggtgggat agacctcatc gtcagcatcg acaaaagcga
    18121 catatttgcc cgtcgccacc tcgatcccac gattacgcgc taccgatgcg ccagcattcg
    18181 cctgatgcaa caaacgaacg tgcggatagt tttctgcgta atgctttgct atttcaacag
    18241 aattatccgt tgaaccatcg ttaataataa tgatttccag agcagtccag gtttgcgtaa
    18301 ttaaagattc catacaag
//
LOCUS       DBFIFI010000078        18181 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.078, whole genome shotgun sequence.
ACCESSION   DBFIFI010000078 DBFIFI010000000
VERSION     DBFIFI010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18181
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.078"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..82
                     /locus_tag="N7B39_002558"
     CDS             <1..82
                     /locus_tag="N7B39_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2422520.1"
                     /translation="EPSTMELKVKTLRYFNELEAENIKGL"
     gene            142..1290
                     /gene="nagA"
                     /locus_tag="N7B39_002559"
     CDS             142..1290
                     /gene="nagA"
                     /locus_tag="N7B39_002559"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HGU2422521.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            1299..2519
                     /gene="nagC"
                     /locus_tag="N7B39_002560"
                     /gene_synonym="nagR"
     CDS             1299..2519
                     /gene="nagC"
                     /locus_tag="N7B39_002560"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HGU2422522.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            2567..3319
                     /gene="nagD"
                     /locus_tag="N7B39_002561"
     CDS             2567..3319
                     /gene="nagD"
                     /locus_tag="N7B39_002561"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HGU2422523.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            3576..5240
                     /gene="asnB"
                     /locus_tag="N7B39_002562"
     CDS             3576..5240
                     /gene="asnB"
                     /locus_tag="N7B39_002562"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HGU2422524.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDKA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            5620..5696
                     /locus_tag="N7B39_002563"
     tRNA            5620..5696
                     /locus_tag="N7B39_002563"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5654..5656,aa:Met,seq:cat)
     gene            5707..5791
                     /locus_tag="N7B39_002564"
     tRNA            5707..5791
                     /locus_tag="N7B39_002564"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5741..5743,aa:Leu,seq:tag)
     gene            5815..5889
                     /locus_tag="N7B39_002565"
     tRNA            5815..5889
                     /locus_tag="N7B39_002565"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5847..5849,aa:Gln,seq:ttg)
     gene            5924..5998
                     /locus_tag="N7B39_002566"
     tRNA            5924..5998
                     /locus_tag="N7B39_002566"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5956..5958,aa:Gln,seq:ttg)
     gene            6014..6090
                     /locus_tag="N7B39_002567"
     tRNA            6014..6090
                     /locus_tag="N7B39_002567"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6048..6050,aa:Met,seq:cat)
     gene            6139..6213
                     /locus_tag="N7B39_002568"
     tRNA            6139..6213
                     /locus_tag="N7B39_002568"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6171..6173,aa:Gln,seq:ctg)
     gene            6251..6325
                     /locus_tag="N7B39_002569"
     tRNA            6251..6325
                     /locus_tag="N7B39_002569"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6283..6285,aa:Gln,seq:ctg)
     gene            complement(6479..7654)
                     /gene="ubiF"
                     /locus_tag="N7B39_002570"
     CDS             complement(6479..7654)
                     /gene="ubiF"
                     /locus_tag="N7B39_002570"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HGU2422525.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFTVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGEVIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQVEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            7800..9224
                     /gene="miaB"
                     /locus_tag="N7B39_002571"
     CDS             7800..9224
                     /gene="miaB"
                     /locus_tag="N7B39_002571"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HGU2422526.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            9377..10417
                     /gene="ybeZ"
                     /locus_tag="N7B39_002572"
     CDS             9377..10417
                     /gene="ybeZ"
                     /locus_tag="N7B39_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HGU2422527.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            10414..10881
                     /gene="ybeY"
                     /locus_tag="N7B39_002573"
     CDS             10414..10881
                     /gene="ybeY"
                     /locus_tag="N7B39_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HGU2422528.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQMVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            10971..11849
                     /gene="corC"
                     /locus_tag="N7B39_002574"
     CDS             10971..11849
                     /gene="corC"
                     /locus_tag="N7B39_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HGU2422529.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            11874..13412
                     /gene="lnt"
                     /locus_tag="N7B39_002575"
     CDS             11874..13412
                     /gene="lnt"
                     /locus_tag="N7B39_002575"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HGU2422530.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIRWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            13809..14717
                     /gene="gltI"
                     /locus_tag="N7B39_002576"
     CDS             13809..14717
                     /gene="gltI"
                     /locus_tag="N7B39_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HGU2422531.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            14887..15627
                     /gene="gltJ"
                     /locus_tag="N7B39_002577"
     CDS             14887..15627
                     /gene="gltJ"
                     /locus_tag="N7B39_002577"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HGU2422532.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQASKLLDYSAHAWESFTAITLA
                     YVFINAFIMLVMTLVERKVRLPGNMGGK"
     gene            15627..16301
                     /gene="gltK"
                     /locus_tag="N7B39_002578"
     CDS             15627..16301
                     /gene="gltK"
                     /locus_tag="N7B39_002578"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HGU2422533.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            16301..17026
                     /gene="gltL"
                     /locus_tag="N7B39_002579"
     CDS             16301..17026
                     /gene="gltL"
                     /locus_tag="N7B39_002579"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HGU2422534.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            17144..18079
                     /gene="rihA"
                     /locus_tag="N7B39_002580"
     CDS             17144..18079
                     /gene="rihA"
                     /locus_tag="N7B39_002580"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HGU2422535.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
BASE COUNT         4408 a         4710 c         4716 g         4347 t
ORIGIN      
        1 tgagccttcc accatggagc tgaaagttaa gactttaaga tatttcaatg aattagaagc
       61 agaaaatatc aaaggtctgt aattgttatc cctgccctgg ctccttgctc agggcaatat
      121 tttttaaaat cgggggtcag aatgtatgca ttaacccagg gccggatctt taccggccac
      181 gaatttcttg atgaccacgc ggttgttatc gctgatggcc tgattaaaag cgtctgtccg
      241 gtagcggaac tgccgccaga gatcgaacaa cgttcactga acggggccat tctctccccc
      301 ggttttatcg atgtgcagtt aaacggctgc ggcggcgtac agtttaacga caccgctgaa
      361 gcggtcagcg tggaaacgct ggaaatcatg cagaaagcca atgagaaatc aggctgtact
      421 aactatctgc cgacgcttat caccaccagc gatgagctga tgaaacaggg cgtgcgcgtt
      481 atgcgcgagt acctggcaaa acatccgaat caggcgttag gtctgcatct ggaaggcccg
      541 tggctgaatc tggtgaaaaa aggtacccat aatccgaatt ttgtgcgtaa gcctgatgcc
      601 gcgctggtcg atttcctgtg tgaaaacgcc gacgtcatta ccaaagtgac cctggcaccg
      661 gaaatggttc ctgcggaagt catcagcaaa ctggcaaatg ccgggattgt ggtttctgcc
      721 ggtcactcca acgcgacgtt gaaagaagcg aaagccggtt tccgcgcggg gattaccttt
      781 gccacccatc tgtacaacgc gatgccgtat attaccggtc gtgaaccggg cctggcgggc
      841 gcgatcctcg atgaagctga catttattgc ggtattatcg ctgatggcct gcatgttgat
      901 tacgccaaca ttcgtaatgc taaacgcctt aaaggcgaca aactgtgtct ggttaccgac
      961 gccaccgcgc cagcaggtgc caacattgaa cagttcattt ttgcgggtaa aacaatatac
     1021 taccgtaacg gactttgtgt ggatgagaac ggtacgttaa gcggttcatc cttaaccatg
     1081 attgaaggcg tgcgtaatct ggtcgaacat tgcggtatcg cactggatga agtgctgcgt
     1141 atggcgacgc tctatccggc gcgtgcgatt ggcgttgaga aacgtctcgg cacactcgcc
     1201 gcaggtaaag tagccaacct gaccgcattc acacctgatt ttaaaatcac caagaccatc
     1261 gttaacggta acgaggtcgt aactcaataa gagaaagtat gacaccaggc ggacaagctc
     1321 agataggtaa tgttgatctc gtaaaacagc ttaacagcgc ggcggtttat cgcctgattg
     1381 accagtacgg gccaatctcg cggattcaga ttgccgagca aagccagctt gcccccgcca
     1441 gcgtaaccaa aattacgcgt cagcttatcg aacgcgggct gatcaaagaa gttgatcagc
     1501 aggcctccac cgggggccgc cgcgctatct ctatcgtcac cgaaacccgc aatttccacg
     1561 caatcggcgt acggcttggt cgtcacgacg ccaccatcac cctgtttgat ctcagcagca
     1621 aagtgctggc agaagaacat tacccgctgc cggaacgtac ccagcagacg ctggaacatg
     1681 ccctgctgaa tgccattgct cagtttattg atagctacca gcgcaaactg cgcgagctga
     1741 tcgccatttc cgttatcctg ccaggacttg ttgacccgga cagcggcaaa attcattaca
     1801 tgccgcatat tcaggtagaa aactgggggc tggtagaagc actggaagag cgttttaaag
     1861 tgacctgttt cgttggtcac gatatccgta gtctggcgct ggcggagcac tacttcggtg
     1921 caagtcagga ttgcgaagac tccattctgg tgcgtgtcca tcgcggaacc ggggccggga
     1981 ttatctctaa cgggcgcatt tttattggcc gcaacggcaa cgtcggtgaa attggccata
     2041 ttcaggtcga accgctgggt gaacgctgcc actgcggcaa ctttggctgc ctggaaacta
     2101 tcgctgccaa cgccgctatt gaacaacggg tgttgaatct gttaaagcag ggctaccaga
     2161 gccgcgtgcc gctggacgac tgcaccatca aaactatctg caaagccgcg aacaaaggcg
     2221 atagcctggc ctcggaagtg attgagtatg tcggtcgtca tctgggcaaa accatcgcca
     2281 ttgctatcaa cttatttaat ccgcaaaaaa ttgttattgc cggtgaaatc accgaagccg
     2341 ataaagtgct gctccctgct attgaaagct gcattaatac tcaggcgctg aaggcgtttc
     2401 gtactaatct gccggtggta cgttctgagc tagaccaccg ctcggcaatc ggtgcttttg
     2461 cgctggtaaa acgcgccatg ctcaacggta ttttgctcca gcatttgctg gaaaattaat
     2521 gtgcttttat agtggcgctt attgttgtca atattctggg tagtccatga ccattaaaaa
     2581 tgtaatttgc gatatcgacg gcgtgctgat gcacgataac gtcgccgtac cgggtgcagc
     2641 ggaatttttg cacgggatta tggataaagg cctgccgctg gtgttgctga ccaactatcc
     2701 ttcgcagacc gggcaagatc tggcgaaccg ctttgccact gccggtgtcg atgtacctga
     2761 cagcgtgttt tatacctctg cgatggcgac cgccgatttc ctgcgtcgcc aggaaggcaa
     2821 gaaagcgtat gttgtgggcg aaggcgcact gattcatgaa ctgtacaaag ccggtttcac
     2881 tattaccgat gtgaaccctg attttgtgat tgttggcgaa acgcgttcct acaactggga
     2941 catgatgcat aaagcggcct atttcgtcgc taacggtgca cgctttatcg ccaccaaccc
     3001 ggacacccac gggcgcggtt tttatccggc ctgtggcgcg ttgtgtgcag ggattgagaa
     3061 aatctccggg cgcaaaccgt tctatgttgg taagccgagt ccgtggatta tccgcgcggc
     3121 attaaacaaa atgcaggcac attcggaaga aacggtgatt gtcggcgata acctgcgtac
     3181 cgatattctg gccggcttcc aggcaggtct ggagacgatt ctggtgcttt ctggtgtttc
     3241 gtcgctcgac gatatcgaca gtatgccttt ccgccccagc tggatttacc cgtcagtcgc
     3301 tgaaatcgac gttatctgaa tagcaaccac gtctcaggac gtggtttctt atttttaccc
     3361 atcattaaaa tcatcccatt caataaaacc cgccaaaaat tatcgatcca tcaataaata
     3421 catcaccgca ccctcccctt tttcacaaat cagcaatcac cattacgttt tttatttttt
     3481 caccgccaaa tcgcttgcgc acagtgctgc tttgcggcat ttttttaaac aagcaaacac
     3541 aacaagcaac aaataccagg ttaacggaga aggttatgtg ttcaattttt ggcgtattcg
     3601 atatcaaaac agacgcagtt gagctgcgta agaaagcact cgagctgtca cgcctgatgc
     3661 gtcatcgtgg cccggactgg tccggtattt atgccagcga taaagccatt ctcgctcacg
     3721 aacgtctgtc aattgttgac gttaacgcgg gggcgcaacc tctctacaac caacaaaaaa
     3781 cccacgtact ggcggtaaac ggtgaaatct acaatcacca ggcattgcgc gctgaatatg
     3841 gcgatcgtta tcagttccag acagggtctg actgtgaagt gatcctcgcg ctgtatcagg
     3901 aaaaagggcc ggaatttctc gacgacttgc agggcatgtt tgcctttgcc ctgtacgaca
     3961 gcgaaaaaga tgcctacctg attggtcgcg accatctggg gatcatccca ctgtatatgg
     4021 gctatgacga acacggtcag ctgtatgtgg cctcagaaat gaaagccctg gtgccagttt
     4081 gccgcacgat taaagagttc ccggcgggga gctatttgtg gagtcaggac ggcgaaattc
     4141 gttcttatta tcatcgcgac tggttcgact ttgatgcagt gaaagataac gtgaccgaca
     4201 aaaacgagct gcgtcaggct ctggaagatt ccgttaaaag ccatctgatg tctgatgtgc
     4261 cttacggtgt gctgctttct ggtggtctgg attcctcaat tatttccgct atcaccaaga
     4321 aatacgcagc ccgtcgcgtg gaagatcagg aacgctctga agcctggtgg ccgcagttac
     4381 actcctttgc tgtaggtctg ccgggttcac cggatctgaa agcagcccag gaagtggcaa
     4441 accatctggg cactgtgcat catgaaattc acttcactgt acaggaaggt ctggacgcca
     4501 tccgcgacgt gatttaccac atcgaaacct atgatgtgac cactattcgc gcttcaacac
     4561 cgatgtattt aatgtcgcgt aagatcaaag cgatgggcat taaaatggtg ctgtccggtg
     4621 aaggttctga tgaagtgttc ggcggttatc tttacttcca caaagcaccg aatgccaaag
     4681 aactgcatga agagacggtg cgtaaactgc tggccctgca tatgtatgac tgcgcgcgtg
     4741 ccaacaaagc gatgtcagcc tggggcgtgg aagcacgcgt tccgttcctc gacaagaaat
     4801 tcctcgacgt ggcgatgcgc attaacccgc aggataaaat gtgcggtaac ggcaagatgg
     4861 aaaaacacat cctgcgtgaa tgttttgagt catacctgcc cgcaagcgtg gcctggcggc
     4921 agaaagagca gttctccgat ggcgtcggtt acagttggat cgacaccctg aaagaagtgg
     4981 cggcgcagca ggtttctgat caacagctgg aaactgcccg cttccgcttc ccgtacaaca
     5041 caccgacctc aaaagaagcg tatctgtacc gggagatctt tgaagaacta ttcccgcttc
     5101 cgagcgccgc tgagtgcgtg ccgggcggtc cttccgtcgc gtgttcttcc gctaaagcga
     5161 tcgaatggga tgaagcgttc aagaaaatgg acgatccgtc tggtcgcgcg gttggtgttc
     5221 accagtcggc gtataagtaa gaaatgcgaa acagccccgg tgaaatccgg ggctgtttca
     5281 gttattgagg tgcttgttga ttctgatatg cagtaaggaa atgttccggg tttgccacat
     5341 cactgtttga caactcatca ctgtaggcca gtatttcata gattaatttc ccaccactat
     5401 tgttaaattc cgaatttcat cctgtatgac aaaagaatgg ctgtagggtg ggaaaaaaca
     5461 caaagtaaac aataattgac gaatatagcg ccacgctgtt cgcaacctaa ccaaacagtc
     5521 actttcgagc aattttcctt gaaaaagagg ttgacgctgc aaggctctat acgcataatg
     5581 cgccccgcaa cgccgataag gtatcgcgaa aaaaaagatg gctacgtagc tcagttggtt
     5641 agagcacatc actcataatg atggggtcac aggttcgaat cccgtcgtag ccaccatctt
     5701 ttttttgcgg gagtggcgaa attggtagac gcaccagatt taggttctgg cgccgcaagg
     5761 tgtgcgagtt caagtctcgc ctcccgcacc attcaccaga aagcgttgta cggatggggt
     5821 atcgccaagc ggtaaggcac cggtttttga taccggcatt ccctggttcg aatccaggta
     5881 ccccagccat cttctttgag taagcggttc accgcccggt tattggggta tcgccaagcg
     5941 gtaaggcacc ggtttttgat accggcattc cctggttcga atccaggtac cccagccatc
     6001 gaagaaacaa tctggctacg tagctcagtt ggttagagca catcactcat aatgatgggg
     6061 tcacaggttc gaatcccgtc gtagccacca aattctgaat gtgtcgaata cgttcggcaa
     6121 attcaaaaac caatttgttg gggtatcgcc aagcggtaag gcacaggatt ctgattccgg
     6181 cattccgagg ttcgaatcct cgtaccccag ccaatttatt caagacgctt accttgtaag
     6241 tgcacccagt tggggtatcg ccaagcggta aggcaccgga ttctgattcc ggcattccga
     6301 ggttcgaatc ctcgtacccc agccacatta aaaaagctcg cttcggcgag ctttttgctt
     6361 ttctgcgttc actcaatgtc gaatgcgatg ttgacacgtc ttatccttca atgtcggatg
     6421 cgacgctgcc gcgtcttatc cgacctacgg ttggcacgca tccggcaatg ttgtaaggct
     6481 acaaccctaa cgcatatttc agcgcctgac gtttcaacac gccagcacgc tccgccgcca
     6541 tcaaaccaag attacgcaca aaacgcagcg gcggcagatt attactgaat ccggcataaa
     6601 acagatccat accgctctgc ataatgaagt tatccgccat gcgccgcatc tggtaacgct
     6661 tgaggacagg ataactggcc cacgcttcgc cgtagctgcg ggcattcacc agaacatcaa
     6721 tcagggcatc gacatcacga taaccaagat tcaccccctg ccccgccagc ggatggatgg
     6781 tgtgcgcggc atcacccacc agcgcaagcc ccggctgcac atactgcaac gcatggcggc
     6841 gtgtcagcgg aaacgcacca gcggcaagcg gtgtaacgta acccagacgc gacgggaaat
     6901 gcttcgcgat ttccacctgt aactgcgcca tattcatatt ctgcaactgg cgaatgcgcg
     6961 ccggagagtc gtaccacacc agcgatgccc agttatcaaa cagcggcaga aacgcgcgcg
     7021 gtccatccgg ggtaaattgc tgccaggtgc tatcgccggg atcgttctcg cactggacgc
     7081 taatcaacat gcacgactgc gcatactgcc atgcatgaac gccaattccc gccatctgcc
     7141 gcacctgcga atttgcgccg tcggcaccaa tcaccagctt cgcgcgaatc acttcaccgc
     7201 ctttcagctc cagctcctga agatcattat ctcgatgtaa tgcaatcagc gaggttggca
     7261 cacgtaacgt tactttcgga tgcgcttcca gcgcctgcca caatgcctgt tgcaggacag
     7321 tgttttctac catatagcca agcaacggca actttaattc ggctgcgtca aataccacat
     7381 gcgccgtttc ccactcccac gtttccagtc tgcggtaagg atggcaacgc atagcctgta
     7441 ctgcatccca gacccctaac cctttaagca atgataccga agccgcgctg atcgccgaga
     7501 tccgcacgtc cggttggcta tcagcgacaa acggtgcagg ttctgcgtgc tcgatcaccg
     7561 ttaccgtaaa tccgtgctgt gccagcccca gcgccagtgc gccgccgacc attcctccgc
     7621 cgacaatggc aatttccgtt ggttgatttg tcatggtcaa tcatcctgtt agcaatatgc
     7681 tttaagttta caggattttt acccccaagg gctgataacg ctcatactgg tcacaacggc
     7741 agcaaagcat tacactatgc gccctgcatt cctggctact atttcgcaag agcaagtcga
     7801 tgaccaaaaa actccatatt aaaacctggg gctgtcagat gaacgagtac gattcatcga
     7861 agatggccga tctgctggat gccacccacg gctatcaact gaccgacgtg gcggaagaag
     7921 cggatgtgct gctgctgaac acctgctcaa tccgcgagaa ggctcaggaa aaagtcttcc
     7981 atcagttggg tcgctggaaa ctgttaaaag agaagaatcc agacctgatt atcggcgtcg
     8041 gtggctgcgt ggcatcgcaa gaaggcgagc acattcgcca gcgcgcccac tatgtcgata
     8101 ttatttttgg gccgcaaacg ctgcaccgtc tgccggagat gatcaactcc gtgcgcggcg
     8161 accgcagccc ggttgtagat atcagcttcc cggaaatcga gaagtttgac cgtctaccgg
     8221 aaccgcgcgc cgaagggccg accgcgtttg tctccatcat ggaaggctgc aataaatatt
     8281 gcacctactg cgtggtgcct tacacccgtg gtgaagaggt aagccgtccg tctgacgata
     8341 ttctgtttga gattgcccag cttgcggctc agggcgtgcg tgaagtcaac ctgctcggtc
     8401 agaacgtgaa cgcctggcgt ggtgaaaact acgacggcac caccggatcg tttgccgatc
     8461 tgttgcgtct ggttgctgcg atcgacggga tcgatcgtat tcgctttacc accagccatc
     8521 cgatcgaatt caccgacgat atcatcgaag tgtatcgcga cacgccggag ctggtgagct
     8581 tcctgcatct gccggtacag agcggttccg atcgcattct gaacctgatg ggacgtaccc
     8641 atacggcgct ggagtacaaa gcgatcatcc gtaaactgcg tgcggcgcgt ccagatattc
     8701 agatcagttc tgacttcatc gtcggcttcc ctggcgaaac caccgaagac ttcgagaaaa
     8761 cgatgaagct gattgccgac gtcaatttcg acatgagcta cagctttatc ttctctgcac
     8821 gtccgggtac accagccgcc gatatggttg atgatgttcc ggaagaagag aagaagcagc
     8881 gtctgtatat tctgcaagaa cgtatcaatc agcaagcgat ggcgtggagc cgccggatgc
     8941 tcggcaccac ccagcgtatt ctggtagaag gtacatcgcg taagagcatc atggagcttt
     9001 ccgggcgtac ggaaaataac cgcgtggtca acttcgaagg cacgccggat atgatcggta
     9061 aattcgtcga tgtagaaatt accgacgtct acccgaactc tctgcgcggt aaagtggtgc
     9121 gtactgaaga tgaaatgggt ctgcgcgtgg cagaaacacc agaatcggtg attgcccgta
     9181 ctcgcaaaga aaacgacctt ggcgtgggtt attatcagcc gtaattctca ggcccgccgt
     9241 tccggtgggc cttgtttttt ctctctttat ccccatcttt atcgcaatgc ttgcgccttt
     9301 ttcctgtggc gtgaataatt tccttatagg ccggtgaatg ttcagcttcc ggcggcatac
     9361 agataagagg aacggtttga acatagacac tcgcgaaatc accctggagc cagcagacaa
     9421 cgcgcgtctg ttgagcctgt gcggcccgtt tgatgacaac atcaagcagc tcgaacgccg
     9481 tctcggcatc gagatcaatc gccgcgataa ccactttaaa ctgaccggcc gtccgatttg
     9541 cgtcaccgct gcggcagaca ttctgcgtag cctgtatgtc gatactgccc cgatgcgcgg
     9601 tcagattcag gatatcgaac cggaacagat ccaccttgcg attaaagaag cacgggtact
     9661 ggagcaaagc gcggagagcg tgccggagta cggcaaagcg gtcaatatca aaaccaaacg
     9721 cggcgtaatt aagccgcgca cgccaaacca ggcgcagtac atcgccaata ttctcgacca
     9781 tgacattacc ttcggcgttg gcccggcggg tacgggtaaa acctacctgg cagtggctgc
     9841 ggcagttgat gccctggagc gtcaggaaat tcgccgtatt ctgctgactc gtccggcagt
     9901 agaagccggt gagaaactgg gcttcctgcc tggcgattta agccagaaag tagacccgta
     9961 tctgcgcccg ctgtacgacg cgctgtttga aatgctgggt tttgagaaag tcgagaaact
    10021 gattgagcgc aacgttattg aagtcgcacc gctggcctat atgcgtggtc gtacgctgaa
    10081 cgacgcattt atcattctcg atgagagcca gaacactacc atcgaacaga tgaagatgtt
    10141 cctgacccgt atcggtttta actcaaaagc ggttatcacc ggcgacgtca cacagatcga
    10201 cctgccgcgt aatactaaat caggcttacg tcatgccatc gaagtgctgg ccgatgtcga
    10261 agagatcagc tttaacttct tccacagcga agacgtggtt cgtcacccgg tggtggcgcg
    10321 tatcgttaac gcctatgaag cctgggaaga agccgaacaa aaacgtaaag cggcgctggc
    10381 ggcagaacgc aagcgcgaag aacaggaaca aaaatgagtc aggtgatcct cgatttacaa
    10441 ctggcatgtg aagataactc cggattgccg gaagagagcc agtttcaaac atggctgaat
    10501 gcggtgatcc cgcagtttca ggaagagtcg gaagtgacga ttcgcgtggt cgataccgcc
    10561 gaaagccaca gtctcaatct gacctatcgc ggtaaggata agccgaccaa cgtgctctcc
    10621 ttcccgtttg aagtgccgcc tggcatggag atgtcgctac tgggcgatct ggttatctgc
    10681 cgtcagatgg ttgagaagga agctcaggag caaggcaaac cactggaggc gcactgggcg
    10741 catatggtgg tgcacggcag tctgcatttg ttaggttacg atcacatcga agatgacgaa
    10801 gcagaagaaa tggaagccct cgaaacagag attatgcttg ctctgggcta tgaggatccg
    10861 tacattgccg agaaagaata atttgccagc catttgactg gcagacgtcc ccgccgttgg
    10921 tgattaacga cggcgttttg attaactaac atgagaaccc atacgacgcc atgagcgacg
    10981 acaattcaca cagtagtgac acgataagca acaagaaggg atttttctcc ctgttactca
    11041 gccaactttt ccacggtgaa ccgaaaaacc gtgacgaact gctggcgctg atccgtgatt
    11101 ccgggcagaa cgaccttatc gacgaagata cgcgcgatat gctcgaaggg gtgatggaca
    11161 tcgcagacca acgcgtccgc gacatcatga tcccccgctc ccagatgatt accctgaaac
    11221 gcaaccagac gctggacgaa tgccttgatg tcatcatcga gtccgcccac tcacgtttcc
    11281 cggtgattag cgaagacaaa gatcacattg aagggattct gatggcgaaa gacttgctgc
    11341 cgtttatgcg cagcgatgct gaagccttca gcatggacaa agtgttacgt caggcggttg
    11401 tcgttcctga aagtaagcgc gtagaccgga tgctgaaaga gtttcgctct cagcgttacc
    11461 acatggcgat cgttattgac gaattcggtg gggtttccgg tctggtaacc attgaagaca
    11521 tcctggaact gattgttggt gagattgaag acgaatatga cgaagaagat gatatcgact
    11581 tccgtcagct gagtcgtcat acctggaccg tgcgcgcact ggcttccatt gaagacttca
    11641 acgaagcgtt cggcacccac tttagcgatg aagaagtcga cactatcggt ggtctggtga
    11701 tgcaggcatt tgggcatctt ccggcacgtg gcgaaaccat cgacatcgac ggttaccagt
    11761 tcaaagtggc gatggccgac agtcggcgta ttattcaggt tcatgtcaaa atcccggatg
    11821 actcacccca gccgaagctg gatgaataaa accgaaactg gatagataac tacatggctt
    11881 ttgcctcatt aattgaacgc cagcgcattc gcctgctgct ggcgttatta ttcggtgcct
    11941 gcggaacgct ggccttctct ccttacgacg tctggcctgc ggcgattatt tcgctgatgg
    12001 ggcttcaggc gttgaccttt aaccgccgtc cactccagtc tgccgctatt ggcttttgct
    12061 ggggatttgg cctctttggc agcggtatta actgggtcta tgtcagcatc gcgacctttg
    12121 gcggaatgcc tggcccggtt aacatcttcc tggtggtact gctggcggcg tatttgtcgc
    12181 tgtataccgg actgtttgct ggcgtgctgt cgcgcctgtg gccgaaaacc acctggctgc
    12241 gcgtggcgat tgccgcccct gctctctggc aagtgaccga gtttctgcgc ggttgggtac
    12301 tgacaggctt cccgtggtta cagttcggct atagccagat tgatggcccg ttaaaagggc
    12361 tggcaccgct aatgggcgtg gaagccatta acttcctgct tatgatggtt agcggcctgc
    12421 tggcactggc gttagtcaaa cgcaactggc gtccgctggt ggtggccgtc gtgctgtttg
    12481 cccttccctt cccgctgcgt tacatccggt ggtttacccc acaaccggag aaaaccattc
    12541 aggtttcgat ggttcagggc gatattccgc aatcgctgaa atgggacgaa ggccagcttc
    12601 ttaatacgct gaagatttac tacaacgcaa cggcaccgct gatgggcaaa tcatcgttga
    12661 ttatctggcc ggagtcggcg ataaccgatc tggaaattaa tcagcaaccg ttcctcaaag
    12721 cactggacgg tgagttgcgt gataaaggta gctcgctggt gaccgggatt gttgacgcgc
    12781 gtcttaataa gcagaaccgt tacgatacct acaacaccat catcacactg ggtaaaggtg
    12841 caccgtacag ctacgaatca gccgatcgct ataacaaaaa ccatctggtg ccgtttggcg
    12901 agtttgtccc gctggagtcg attctgcgtc cgttagcacc gttctttgat ctgccgatgt
    12961 cgtcgttcag ccgtgggcca tatatccagc cgccgctgtc ggcaaatggt attgagctta
    13021 ctgcggctat ttgctacgag atcattctcg gcgagcaagt gcgcgataac ttccgcccgg
    13081 ataccgacta tctgctgact atctccaacg atgcgtggtt tggtaagtct attggtccat
    13141 ggcagcactt ccagatggcg cgaatgcgtg cgctggagct ggcgcgccca ctgttgcgca
    13201 gcaccaacaa cggcattacg gcggtgattg gcccgcaggg tgagattcag gcgatgatcc
    13261 cgcagttcac ccgcgaggtg ttaaccacta acgtgacgcc gaccaccgga ctcacaccat
    13321 acgcacgtac cggcaactgg ccgctgtggg tgctgacggc actgtttggt tttgctgctg
    13381 tgttgatgag tctgcgtcag cgacgtaaat aatcccttct tatcgtgccg gaatacatcc
    13441 catatttcgg cacgctcacc ggatgcggca tcagtgcatt atccggataa caatattctc
    13501 ctatccaact ttggcacatc tattgctttg ttatacaagg caaagcttta accagcatca
    13561 gtacaacccg gttgcaccac agcaatccac cggtagcacc aaaagagtgc attttaagaa
    13621 ttttgcttcg gaatggtgcg gcgcacctcc caaatataaa caataaaaca gcaatatctt
    13681 tacatttagc caaactaaat gttacctaac aggcacaaca ctacacaata aagttgcaga
    13741 tgataacaac acaaacactc acaacgggta tccatgcgtt cttaacgcag aagataaagg
    13801 agttggatat gcaattacgt aaacctgcca cagcaatcct ggccctggcg ctttccgcag
    13861 gactggcaca ggcagatgac gccgccccgg cagcgggcag tacgctggac aaaatcgcca
    13921 aaaacggcgt gattgtcgtc ggtcaccgtg aatcttcagt acctttctct tattacgaca
    13981 atcagcaaaa agtggtgggt tattcgcagg attactccaa cgccattgtt gaagcagtga
    14041 aaaagaaact caacaaaccg gacttgcagg tgaaactgat tccgattacc tcacaaaacc
    14101 gtattccgct gctgcaaaac ggcacttttg attttgaatg tggttctacc accaacaacg
    14161 tcgaacgcca aaaacaggcg gctttctctg acactatttt cgtggtcggt acgcgcctgt
    14221 tgaccaaaaa gggcggcgat atcaaagatt ttgccgacct gaaaggcaaa gccgtagtcg
    14281 tcacttccgg cactacctct gaagttttgc tcaacaaact gaatgaagag caaaaaatga
    14341 atatgcgcat catcagcgcc aaagatcacg gtgactcttt ccgtaccctg gaaagcggtc
    14401 gtgccgttgc ctttatgatg gatgatgctc tgctggccgg tgaacgtgcg aaagcgaaga
    14461 aaccagacaa ctgggatatc gtcggcaagc cgcagtctca ggaagcctat ggttgcatgt
    14521 tgcgtaaaga cgatccgcag ttcaaaaagc tgatggatga cactatcgct caggtgcaga
    14581 cctccggtga agcggaaaaa tggtttgata aatggttcaa aaatcccatt ccgccgaaaa
    14641 acctgaacat gaatttcgaa ctgtcagacg aaatgaaagc actgttcaaa gaaccgaatg
    14701 acaaagcact gaactaatta caagaaccag gggcggaaat tctagccctc tcgattgtta
    14761 cgtagcacgg acagactata cgcctgatgg tcgttcccca tcgggcctga aaaccgcaat
    14821 acgctgggta acaatcttcg agggtagcag ttaacgctgc taccctcttt tttctggagt
    14881 agatttatgt ctatagactg gaactggggt atttttttac aacaagcccc gttcggcaac
    14941 accacctatc ttggttggat ctggagtggt tttcaggtga cgatcgcttt gtcgatctgc
    15001 gcctggatta tcgctttcct cgtcggttca tttttcggta ttttacgtac cgttccaaac
    15061 cgttttctct ccggtctggg tacgttgtat gtcgaactgt tccgcaacgt gccgttgatt
    15121 gtgcaattct ttacctggta tctggtgatc ccggagctgc tgccggagaa aatcggcatg
    15181 tggtttaagg ccgagctgga tcccaatatt cagtttttcc tttcatccat gctctgcctg
    15241 gggctgttta ccgccgcccg tgtttgcgaa caggtacgcg ccgcgattca gtcgctgccg
    15301 cgtgggcaga agaatgccgc gctggctatg ggtctgacgc tgccgcaggc ttaccgctac
    15361 gtactgctgc ctaatgctta tcgcgttatc gtcccgccga tgacctcaga gatgatgaac
    15421 ctggtgaaaa actccgccat cgcctcgacc atcggtctgg tagatatggc agcgcaggcg
    15481 agtaaattgc tcgattactc ggctcacgcc tgggagtcgt ttaccgccat tacgctagcg
    15541 tatgtgttta ttaacgcttt tatcatgctg gtgatgacgc tggttgaacg taaagttcgc
    15601 ctgcctggca acatgggggg caaataatgt acgagtttga ctggagttcc attgtccctt
    15661 ccctgccata tctgctcgac gggctggtga tcacgctgaa aatcaccgtc acggcagtcg
    15721 tgattggtat tttgtggggc accatgctgg cggtgatgcg cttgtccagc tttgcgcccg
    15781 tagcgtggtt tgccaaagcc tacgttaacg tattccgctc gattccttta gttatggttt
    15841 tgctgtggtt ttacctgatc gtgccgggtt ttctgcaaaa cgtgctggga ttatcaccaa
    15901 aaaatgatat ccgtttaatt tcggcgatgg tggcgttttc gatgtttgaa gcggcctact
    15961 attcagagat tatccgtgcc ggtattcaaa gtatttctcg tggtcagtcg agcgccgcgc
    16021 tggcgttggg aatgactcac tggcagtcga tgaaactgat tattctgccg caggcgttcc
    16081 gcgcgatggt gccgctgctg ctcactcagg gcatcgtact gttccaggat acctcgctgg
    16141 tgtatgtgtt atctctggcc gatttcttcc gtaccgcctc aaccattggt gagcgtgatg
    16201 gcacgcaggt tgagatgatc ctgtttgccg gatttgttta tttcgttatt agccttagcg
    16261 cgtcgttgtt ggtcagctac ttgaaaagaa ggacagcata atgattaccc tgaaaaatgt
    16321 ttcaaaatgg tatggtcact ttcaggtgct gaccgactgc tcaaccgaag tgaaaaaagg
    16381 cgaagtggtg gtggtttgcg gcccgtctgg ttccggcaaa tcaacgctga ttaaaaccgt
    16441 caacggcctc gaaccagtgc agcaaggtga aatcaccgtc gatggtatcg tggttaacga
    16501 caagaaaacc gatctggcaa agctgcgttc tcgcgtcggg atggtattcc aacatttcga
    16561 gctgttccct catctgtcga ttatcgaaaa cctgaccctg gcgcaggtga aagtgcttaa
    16621 acgcgataaa gcaccggctc gtgaaaaagc attgaaattg ctggagcgcg tgggactttc
    16681 cgcacatgcc aataaatttc cggcgcagct ttccggcggc cagcagcagc gtgtggcaat
    16741 tgctcgcgca ttgtgtatgg atcctattgc gatgctgttc gacgaaccca catcggcact
    16801 agacccggag atgatcaacg aagtactgga cgtgatggtg gaactggcga acgaggggat
    16861 gaccatgatg gtggtgaccc acgaaatggg ctttgcccgt aaagtggcga atcgggtgat
    16921 ctttatggat gagggtaaaa ttgttgaaga ctcgccgaaa gatgcgttct tcgatgatcc
    16981 gaaatcggac cgcgcaaaag acttcctcgc gaaaatcctg cattaatcac tctggcgcgc
    17041 atacccaagt gcgcgccatc tcacgcttta tcgtctcgcc cctcgtatct gttacatcct
    17101 ggcgttaccc ttgtcgcaaa gaagcacaca acaaggagca acaatggcac tgccaattct
    17161 gttagattgc gacccaggtc atgatgacgc tatcgcaata gttctcgccc tcgcctcacc
    17221 agagcttgat gtcaaagcaa ttacgtcttc cgccggaaac cagacaccag aaaaaacctt
    17281 acgcaatgtt ctgcgtatgc tgaccttgct taatcgcacc gatattccgg tggcaggcgg
    17341 cgcggtaaaa ccgttaatgc gtgagttgat tatcgcggac aatgtgcacg gcgaaagcgg
    17401 tctcgacggc ccggcattac cggaaccgac gttcgcaccg caaaactgta cggcggtaga
    17461 gctgatggcg aaaacgctgc gtgaaagtgc ggaacctgtc acgattgtgt ctaccggacc
    17521 gcaaactaac gttgccttgc tgctcaatag ccatccggaa ctgcatagca aaattgcccg
    17581 tatcgtgatt atgggtggcg ctatgggact gggtaactgg acgcctgcgg ctgaatttaa
    17641 tatttacgtt gacccggaag cggcagaaat tgtcttccag tcagggatcc cggtggtgat
    17701 ggccggtctg gatgttactc acaaagcaca aatccacgtt gaagacactg agcgtttccg
    17761 cgcgattggc aaccctgttt caaccattgt tgccgaactg ctggatttct tcctcgaata
    17821 tcataaagac gaaaaatggg gctttgtcgg cgcaccgcta catgacccat gcaccatcgc
    17881 ctggctgtta aaaccggagt tgtttaccac tgttgagcgc tgggttggcg tggaaacaca
    17941 ggggaaatat acccagggta tgacggttgt tgattattat tatctgactg gcaataaacc
    18001 gaatgccact gtaatggtcg atgttgatcg tcagggcttt gttgatttac tggctgatcg
    18061 tctgaaattt tacgcttaac acacgtcatt gattaagatc accacgcgtt acctgggtgg
    18121 tcttaatctg tttatctctt tttctttcaa gatataaatg caatggataa tgcagaactc
    18181 g
//
LOCUS       DBFIFI010000079        18150 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.079, whole genome shotgun sequence.
ACCESSION   DBFIFI010000079 DBFIFI010000000
VERSION     DBFIFI010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18150
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.079"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            220..735
                     /locus_tag="N7B39_002581"
     CDS             220..735
                     /locus_tag="N7B39_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGU2422536.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYHIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            953..1681
                     /gene="artP"
                     /locus_tag="N7B39_002582"
     CDS             953..1681
                     /gene="artP"
                     /locus_tag="N7B39_002582"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HGU2422537.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            1699..2430
                     /gene="artI"
                     /locus_tag="N7B39_002583"
     CDS             1699..2430
                     /gene="artI"
                     /locus_tag="N7B39_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HGU2422538.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITAEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            2437..3153
                     /gene="artQ"
                     /locus_tag="N7B39_002584"
     CDS             2437..3153
                     /gene="artQ"
                     /locus_tag="N7B39_002584"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HGU2422539.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGIVEIPVQMQIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPMGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            3153..3821
                     /gene="artM"
                     /locus_tag="N7B39_002585"
     CDS             3153..3821
                     /gene="artM"
                     /locus_tag="N7B39_002585"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HGU2422540.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            4112..4843
                     /gene="artJ"
                     /locus_tag="N7B39_002586"
     CDS             4112..4843
                     /gene="artJ"
                     /locus_tag="N7B39_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HGU2422541.1"
                     /translation="MKKLVLAALLASCAFGASAAEKINFGVSATYPPFESMDANNQIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPDRSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNSRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQQISDQWFPQ"
     gene            complement(4894..6375)
                     /locus_tag="N7B39_002587"
     CDS             complement(4894..6375)
                     /locus_tag="N7B39_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HGU2422542.1"
                     /translation="MRAIILLFDSLNKRYLPPYGDALTKAPNFQRLAAHAATFENSYV
                     GSMPCMPARRELHTGRCNFLHREWGPLEPFDDSMPELLKKAGIYTHLISDHLHYWEDG
                     GGNYHNRYSSWEIVRGQEGDHWHASVAQPPIPEVLRVPQKQTGGGVSGLWRHDWANRE
                     YIQQEADFPQTKVFDAGCAFIHKNHAEDNWLLQIETFDPHEPFHTTEEYLSLYEDNWD
                     GPHYDWPRGRVQESDEAVEHIRCRYRSLVSMCDRNLGRILDLMDEHDLWRDTMLIVGT
                     DHGFLLGEHGWWAKNQMPYYNEVANNPLFIWNPRSGVKGERRQALVQMIDWAPTLYDF
                     FQQPVPPDVQGQPLAKTVSHDEPVRSSAMFGVFSGHANVTDGRYVYMRAALPGREDDI
                     ANYTLMSCKMNSRYPVDEMRALSLAPPFRFTKGLQVLRIPAQEKYKGLNQFGHLLFDL
                     QNDPQQLHPIHDDVIESRMIALLIQLMKDNDAPPEQFQRLGLA"
     gene            complement(6387..6707)
                     /locus_tag="N7B39_002588"
     CDS             complement(6387..6707)
                     /locus_tag="N7B39_002588"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGU2422543.1"
                     /translation="MKDTINILFVCGYGVGSSVMLQTVVKKALTKYDFKVEMEHTAAG
                     EVGGFTDWADIFAISKKLLDVVSLDPKQGQYLIPIENIMDGETIGQQIYEVVQQHFPH
                     LINQ"
     gene            complement(6729..8072)
                     /locus_tag="N7B39_002589"
     CDS             complement(6729..8072)
                     /locus_tag="N7B39_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000399308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HGU2422544.1"
                     /translation="MEGVPTMFAKFIDVIQTFLTEPAILIGLLVGIGYALDKKSPIKI
                     ITGMVSAMVGLMMVLFGGFQFSATFKPVAEAVSKAYGVHGYLMDSYAMKAATQIALGD
                     NFGYVGYVFVLAFFTNLLLVLFGRYTGAKGIFLTGNTGVSHSQAVLWLIVFWLGFSWT
                     TSIIIAGILTGVFWAFSTTLIVKPIAKVTKDAGFTIAHNQMLGLWFFSKFAHKFGDPE
                     KHDAENLKLPGWLAIFNHNVTAIAIVMTLFVGGFLLSTGIDNVQLMAKGKPWYIYIIN
                     LGLQFSMYMVILLQGVRMMVGEINGSFKGWQDRFIPNAIPAVDVAALLPFSPNAATLG
                     FVFCTFGTIFSMGILLLVHSPIMVLPGFVPLFFSGGPIGVLANRMGGYRSVIICTFLL
                     GIIQTFGTVWAIPLTGLAENGVGWTGIFDWATVWPAICEVLKFIAATFHLGPYAG"
     gene            complement(8352..9479)
                     /gene="rlmC"
                     /locus_tag="N7B39_002590"
     CDS             complement(8352..9479)
                     /gene="rlmC"
                     /locus_tag="N7B39_002590"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HGU2422545.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQA
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALVERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIALEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKM"
     gene            complement(9520..10008)
                     /locus_tag="N7B39_002591"
     CDS             complement(9520..10008)
                     /locus_tag="N7B39_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HGU2422546.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(10068..10913)
                     /gene="potI"
                     /locus_tag="N7B39_002592"
     CDS             complement(10068..10913)
                     /gene="potI"
                     /locus_tag="N7B39_002592"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HGU2422547.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAVISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     CARRG"
     gene            complement(10910..11863)
                     /gene="potH"
                     /locus_tag="N7B39_002593"
     CDS             complement(10910..11863)
                     /gene="potH"
                     /locus_tag="N7B39_002593"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HGU2422548.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNFGNFLQLTGDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(11873..13006)
                     /gene="potG"
                     /locus_tag="N7B39_002594"
     CDS             complement(11873..13006)
                     /gene="potG"
                     /locus_tag="N7B39_002594"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HGU2422549.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLYSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(13101..14213)
                     /gene="potF"
                     /locus_tag="N7B39_002595"
     CDS             complement(13101..14213)
                     /gene="potF"
                     /locus_tag="N7B39_002595"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HGU2422550.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRDNPG
                     IYPPADVRAKLFTLKVQAPKIDRVRTRAWTKVKSGK"
     gene            complement(14564..15040)
                     /locus_tag="N7B39_002596"
     CDS             complement(14564..15040)
                     /locus_tag="N7B39_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HGU2422551.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(15128..16030)
                     /gene="rimK"
                     /locus_tag="N7B39_002597"
     CDS             complement(15128..16030)
                     /gene="rimK"
                     /locus_tag="N7B39_002597"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HGU2422552.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(16091..16813)
                     /gene="nfsA"
                     /locus_tag="N7B39_002598"
     CDS             complement(16091..16813)
                     /gene="nfsA"
                     /locus_tag="N7B39_002598"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HGU2422553.1"
                     /translation="MTPTIELTCGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPSSILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(16797..17084)
                     /locus_tag="N7B39_002599"
     CDS             complement(16797..17084)
                     /locus_tag="N7B39_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HGU2422554.1"
                     /translation="MRAIGKLPKSVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            17244..17501
                     /gene="grxA"
                     /locus_tag="N7B39_002600"
     CDS             17244..17501
                     /gene="grxA"
                     /locus_tag="N7B39_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HGU2422555.1"
                     /translation="MQTVIFGRPGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(17531..17908)
                     /locus_tag="N7B39_002601"
     CDS             complement(17531..17908)
                     /locus_tag="N7B39_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HGU2422556.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
BASE COUNT         4747 a         4775 c         4335 g         4293 t
ORIGIN      
        1 atataacacg ctcaacctca caaatggttt acactgtcta ttgtttgggt aatttaagta
       61 agtcagctac gagcaacctt gagtgggtcg ccagcaatgg actcaaaccc aatcaggtgt
      121 tcagattgtt tcagaattgt attaaaaaca atgtattaaa taatctgata gcgtttggtc
      181 gtagtgtgac gcagacagca agctaataag gaaatgatta tgcgctaccg ttcactatct
      241 ctacttattc catgtgcact gctgctgagt gcctgtacca ccgtcacgcc agcttataaa
      301 gataatggtc cccgcaccgg ttcctgtgtc cagggggggc ctgacagcgt ggcccaacaa
      361 ttctatgact accacattca acatcgcagc aatgacatta ccgccctgcg cccctatttg
      421 agtgacaaac tggcaaccct acttagcgat gccagccgcg ataatagtca tcgtgaattg
      481 ttaagcagtg atccgttctc cagtcgaacc accttgccag acagcgccca cgttgccagt
      541 gcatcgacta tccctaatcg tgatgcccgt aacattccat tgcgcgtgga tctcaagcag
      601 ggtgaccagg gctggcagga tgaagtgtta atgattcagg aaggccagtg ttgggtgatc
      661 gacgatgtgc gctacttagg cggtagcgtt catgccacgg cgggtacgct ccgtcagtcc
      721 atcgagaatc gctaacgcct tgatcaacca ataaaccccg taaattgtcc ggcatttcgt
      781 gaggaatgcc gacatttatg ctcgccgacc accgcccccg ttattttgtg ctatgtttat
      841 tgaataatgc gctttgcttt tagcttttaa agcagaaata ttgcataatt attctgtcaa
      901 aggtactatc ttcgacctca atagctatca gactgccagt atacgagtgt caatgagtat
      961 tcaattaaac ggcattaatt gcttctacgg cgcgcatcag gcgctgttcg atatcacgct
     1021 ggattgccca cagggcgaaa cgctggtgtt acttggcccc agcggcgcgg gtaaaagctc
     1081 gctgctgcgt gtactcaatc tgcttgagat gccgcgctcc ggtacgctca acattgcagg
     1141 caaccatttc gatttcacca aaacaccctc agacaaagcg attcgcgatt tgcgtcgtaa
     1201 cgttggcatg gtgtttcagc aatacaacct gtggccgcat ctgaccgtgc agcaaaacct
     1261 gattgaagcg ccctgccgtg tactggggtt gagtaaagat caggcgctgg cccgtgcaga
     1321 aaaactgctg gaacgtctgc gtctcaaacc ttatagcgat cgttacccgc tgcatctttc
     1381 tggtggtcag cagcagcgtg ttgctattgc ccgtgcgtta atgatggaac cgcaggtact
     1441 gctgtttgat gaaccgaccg ccgcactgga cccggaaatt acggcacaaa tcgtcagcat
     1501 cattcgtgag ctggcagaaa cgaatattac ccaggtgatc gtcacccacg aagttgaagt
     1561 ggcgcgtaaa accgccagcc gagtggtgta tatggaaaac ggtcatatcg tagaacaagg
     1621 cgacgcgagc tgctttaccg agccgcaaac cgaagcattt aaaaactatc tctctcacta
     1681 agattcggga taacaacaat gaaaaaagtt ctgattgccg cgttaattgc aggttttagt
     1741 ctttccgcca cagctgccga aaccattcgt tttgctaccg aagcctccta tcctccgttt
     1801 gaatcgattg atgcaaacaa ccagatcgtt ggttttgacg tcgacctggc acaggcgctg
     1861 tgtaaagaga ttgatgcgac ctgtactttc tctaaccagg catttgacag cctgatccca
     1921 agcctgaaat tccgtcgcgt ggaagcggtg atggccggga tggatatcac cgcggaacgt
     1981 gaaaagcagg tactgtttac cacgccgtac tatgacaact ctgccctgtt tgtgggtcag
     2041 cagggtaaat acaccagcgt tgatcagctg aaaggcaaaa aagtcggcgt acagaatggt
     2101 acgactcacc agaaattcat tatggataag cacccggaaa tcaccaccgt gccgtatgac
     2161 agctaccaga acgcaaaact ggatctgcaa aacggtcgta tcgacagcgt atttggtgac
     2221 accgcagtgg taactgaatg gctgaaagat aacccgaaac tggcggcagt gggcgataaa
     2281 gtgaccgata aagattactt cggtactggc ctcggcatcg cggtacgtca gggcaacact
     2341 gagctgcagc agaaactcaa cactgcgctg gaaaaagtga agaaagatgg cacttacgaa
     2401 accatctaca acaaatggtt ccagaagtaa ttcctgatga atgaattttt tcctttagca
     2461 agcgccgccg ggatgaccgt cggccttgcc gtttgtgcat tgattgtcgg gctggcgctg
     2521 gcgatgttct ttgcggtatg ggagtctgcg aaatggcgtc ctgtcgcgtg ggcaggttca
     2581 gcgctggtaa ccattctgcg tggcctgcca gaaattctgg tggtgctgtt tatctatttt
     2641 ggctcctcgc agctgctgct gacactttcg gatggcttca ctatcaatct tgggattgta
     2701 gagatcccgg tgcagatgca gattgaaaac ttcgacgtta gcccgttcct ttgtggtgtc
     2761 atcgctctgt cactgctgta tgccgcctat gcctcgcaaa cgctgcgcgg cgcgttgaaa
     2821 gcggtgccga tgggtcagtg ggaatccggc caggcgctgg ggctgtcgaa atcggctatc
     2881 tttttccgtc tggtgatgcc gcagatgtgg cgtcatgcgc tgcctggcct cggtaaccag
     2941 tggctggtgc tgctgaaaga taccgcgctg gtcagtttga ttagtgtgaa tgatttaatg
     3001 ctgcaaacca aaagcatcgc tactcgtacc caggaaccat ttacctggta cattgtggcg
     3061 gcggcgattt acctggtgat caccctgctc agtcagtaca ttctcaaacg cattgacctg
     3121 cgcgcgacac gttttgagcg gaggcccagc taatgtttga gtatttaccc gaactgatga
     3181 aagggctgca caccagcctg acgctaaccg ttgcctcgct gattgtggca ctgattctgg
     3241 cgttgatttt taccatcatc ctgacgctga aaacgccggt gctggtgtgg ctggtgcggg
     3301 gttatatcac gctgtttacc ggtacaccgc tgctggtgca gatcttcctg atttattacg
     3361 ggccgggtca gttcccgacg ttgcaggagt atccggcact gtggcatttg ttgtcagaac
     3421 cgtggttatg tgcgctgatt gcgttgtcgc tgaacagtgc ggcgtatacc acgcagctgt
     3481 tttacggtgc aattcgtgcg atcccggaag gtcagtggca gtcctgtagc gccctgggaa
     3541 tgagcaaaaa agatacgctg gcgatcctgc tgccgtatgc ctttaaacgc tcgctctctt
     3601 cttattccaa cgaagtggtg ctggtgttca aaagtacctc tctggcttac accattacgc
     3661 tgatggaagt gatgggatac agccagttgt tgtacggacg cacctacgat gtcatggtgt
     3721 tcggcgcggc agggattatt tatctggtcg ttaacggcct gctgacgctg atgatgcgtc
     3781 tgatcgagcg caaagcgctg gcgtttgagc gaaggaatta atgtggctgc gtacattcgc
     3841 cgggggtttt gtaggtctga taagacgcgc cagcgtcgca tcagacatca gcacggtgcc
     3901 tgtgccggat gcggcgtgga cgccttatcc ggcctacata gtgcataaat tcttataata
     3961 aagacggaca actcactaag ttgtccgtct ttttttattt catgcaaatt atttaatcat
     4021 atttattgca tataaattca cttgatggca ttgttatccc atgccgcaga cacggccaat
     4081 aatcataaga ttgacagacg ggagttccat catgaaaaag ttagttcttg ccgcattact
     4141 ggcatcctgt gcgttcggcg cttctgccgc agagaaaatc aattttggcg tatcagcgac
     4201 ctatccgccg ttcgagtcga tggatgccaa taaccagatc gtcggctttg atatcgacct
     4261 agcaaaagcg ttatgcaaac agatgcaggc agaatgtact tttaccaatc acgcgttcga
     4321 cagcctgatc ccgtcactga aattcaggaa atatgacgcc gtaatctccg gtatggatat
     4381 cacaccagat cgtagcaagc aggtatcgtt caccacgccc tattacgaaa actcagccgt
     4441 cgtgattgcc aaaaaagata cctacaaaac gtttgccgac ctgaaaggca aacgcattgg
     4501 gatggaaaac ggtactacgc accagaaata tattcaggat cagcacccgg aagtgaaaac
     4561 ggtctcctat gacagttatc aaaatgcctt tatcgatctg aaaaatagcc gtattgatgg
     4621 cgtatttggt gacactgcgg tggttaatga atggctgaaa accaatccgc aactgggcgt
     4681 tgctactgaa aaagtgaccg atccgcaata ctttggcacc ggtctgggta tcgctgtacg
     4741 tccggataac aaagctctgc tggaaaaact gaataacgcg ctggcagcaa ttaaagctga
     4801 cggtacatat caacaaatca gcgatcagtg gttcccgcaa taattattca tttgccggag
     4861 tgggcgaaca cccactccgg caaaatcact tctctacgct aatcccaggc gctgaaactg
     4921 ctctggcggc gcatcattat ctttcatcaa ctgaatcagc aacgcgatca tccgggactc
     4981 gatcacatca tcatgaatcg gatgtagctg ctgcggatcg ttttgcagat caaacagcaa
     5041 atgaccaaac tgattcaacc ccttatattt ttcctgtgcc gggatgcgta atacctgtaa
     5101 ccctttggta aaacgaaacg gtggggccag cgataaagcc cgcatctcat ccaccggata
     5161 gcggctgttc attttgcagg acatcaacgt gtagttggca atatcatcct cacgccccgg
     5221 cagcgctgca cgcatataca cataacgccc atcagttacg ttagcatgac cactgaaaac
     5281 accaaacatc gccgagctgc gtactggttc atcgtgactg accgttttcg ccagcggttg
     5341 cccctgcaca tcgggcggca ctggctgttg aaaaaagtca tacaacgttg gcgcccagtc
     5401 gatcatttgt accagtgcct gtcgccgctc tccttttaca ccactgcgcg ggttccagat
     5461 aaacaacgga ttattggcaa cttcgttgta ataaggcatc tggtttttcg cccaccagcc
     5521 atgctcaccg agtaaaaagc cgtggtcagt gccgacaatc agcatggtat ctcgccacag
     5581 atcgtgctca tccatcaggt cgagaatgcg ccccaggttg cgatcgcaca tcgacaccag
     5641 cgaacgatac cggcaacgga tgtgctccac ggcctcgtcg ctttcctgca cccggccacg
     5701 cggccagtca taatgcggtc catcccagtt atcttcatac aaggaaagat actcttccgt
     5761 tgtgtgaaac ggctcgtgcg gatcaaacgt ttcgatctgc aataaccagt tatcttccgc
     5821 gtgatttttg tggataaagg cacagcctgc atcaaacact ttagtttgtg gaaaatccgc
     5881 ttcctgctga atatattccc ggtttgccca gtcatggcgc cacagaccag aaacaccgcc
     5941 accggtctgc ttttgcggca cccgtagcac ctcgggaata ggcggttgcg caacgcttgc
     6001 atgccagtga tcgccttcct gtccgcgcac aatctcccac gaactgtagc gattatgata
     6061 attcccgccg ccatcttccc agtaatgcaa atgatcgctt atcaggtggg tatagatccc
     6121 cgccttttta agcagctccg gcatggaatc gtcaaacggt tctaacggcc cccactcccg
     6181 atgcaggaag ttacatcgcc cggtatgcaa ctcccgccgg gcaggcatac acggcatact
     6241 gccgacgtaa ctgttttcaa aggtggcggc atgagccgcc agacgttgga aattaggcgc
     6301 tttggttaac gcatcgccat aaggtggcag ataacgttta ttcagactat cgaacagcag
     6361 aataatcgct ctcattacgc cctaccttat tgattaatta aatgcggaaa atgttgctga
     6421 accacttcat aaatttgctg accaatagtt tcaccgtcca taatattctc tatcggaatc
     6481 aaatattgcc cttgtttcgg gtcaagacta accacatcga gtaatttttt ggaaatagca
     6541 aaaatatccg cccagtcggt aaatccgccg acttcacctg cggcggtatg ttccatttca
     6601 actttgaaat catatttggt cagcgctttt ttaacgaccg tttgtaacat aacgctactg
     6661 cctacaccgt aaccacagac aaacagaata ttaattgtat ctttcatttt taaaccctta
     6721 atatttaatt aacccgcgta aggaccaaga tggaatgttg cagcgataaa tttcagaact
     6781 tcacaaatag caggccatac ggttgcccag tcaaatattc ctgtccagcc gacgccattt
     6841 tcagcaagcc cggttaacgg aatagcccac accgtaccga aggtctgaat aatacccagc
     6901 aagaacgtac agataattac ggaacggtat ccgcccatgc ggtttgctaa tacgccaatt
     6961 ggaccgccag agaaaaacag aggtacaaag ccaggcaata ccataattgg gctgtgaacc
     7021 agtaataaga tccccatcga aaaaatggtg ccaaaagtac agaagacgaa gcctaacgtt
     7081 gcggcattgg gtgaaaaagg taacagcgct gcaacatcga cagcaggaat agcgttagga
     7141 ataaagcgat cttgccagcc tttaaacgaa ccgttaattt cgcccaccat catgcgcaca
     7201 ccctgcagca gaatgaccat atacatggag aactgtaagc caaggttgat gatatagatg
     7261 taccagggct tgcctttcgc cattaactgg acattatcga tacccgttga tagcaagaaa
     7321 ccaccgacaa acagcgtcat cacaatggcg atagccgtta cgttatggtt aaaaatcgcc
     7381 agccacccgg gaagtttcag gttttcagca tcgtgctttt cagggtcacc aaatttatgg
     7441 gcgaatttgg agaaaaacca caatcccaac atctggttat gagcaatggt aaaaccggca
     7501 tctttggtaa ctttggcaat gggcttaaca atgagcgtgg tggagaacgc ccagaataca
     7561 cccgttaata ttccggcaat aataatggat gtagtccagc taaaacccag ccaaaagaca
     7621 atcaaccata ataccgcctg agaatgtgaa acaccggtat tccctgtcaa aaatatccct
     7681 ttggcaccag tataccgacc aaataatacc agcagcaggt tggtaaaaaa cgccagtaca
     7741 aatacatacc cgacataacc aaaattatcc cccaatgcaa tttgcgttgc cgctttcatt
     7801 gcgtaggaat ccattaaata tccgtgaaca ccataggctt tacttacggc ttctgctacc
     7861 ggtttaaatg ttgcggaaaa ctggaaacca ccaaataaca ccatcattaa tcccaccatg
     7921 gcgctgacca tgccggtaat aatttttatt ggcgatttct tatcaagggc ataaccaata
     7981 cccaccagca aaccaattaa aattgccggt tcagttaaaa acgtttgaat aacatctatg
     8041 aatttggcaa acatcgtcgg aacgccctcc atacgcgtac atttccgttg tcaatgcatg
     8101 aaccttctgc tgacgttaat aatgtctcag gaaattttgt gtttcgctaa gcaatgcctc
     8161 tgtatgtatt cggtaagtgc actgtagtaa tcaaaacagt tcaattaaag atcttatttg
     8221 attatttgtg atttgatttg caaaaataga gcaatatata accgtgatcg atcggttatt
     8281 attaatcgat cgatcacgat tattattttg gatggatgta tgccgaggtg atattgattt
     8341 gaatgatgta tttacatttt taccagcagc gtcagcactt catagtgcgc ggtgtgcggg
     8401 aacatatcaa aaagctgtac ccgttcgatg cggtaacctg gcagttcgcg gatatctttc
     8461 gccatggttt gggcgttaca gctggagtag ataataaaac gcggtgccat cgttgagaga
     8521 taatcacaca gcggtttacc aatgccgcgg cgcggcgggt taaccagcac cagctccggc
     8581 acctccccct gagcggtggc aaactgagtg gagtccagcg cctgaaattg cagacgcgtt
     8641 aagcccagtt cagcggctga ctgctttgca caggcaatgg cctctagcgc aatttcgatc
     8701 ccggttaact gcatgtcagg cgtcgcgcag tgtaaaccaa agccccccac accgcagaac
     8761 agatcccaca tatgtttaac cggcagctgt cgtacccagt cgcgcgcggt agcgtacagc
     8821 tggctggcga ccgccggatt agtctggaag aaactttgcg gacggatcca cagcggcaca
     8881 tcgttaaaac gctctaccag cgcctgttgt tcggtcaggt agatctccgt ctccccttcc
     8941 ataatcgcca tatgtaccgg ctgaatattg acggtgataa cttttagctg cggcaattgt
     9001 gcctgtaacc acggcaatgc cttacgcagt tgtgctagtt tggtttcaga acgcagtaca
     9061 aagcgcagca tcatgccgcc atcgctctgg ctttcagtca gcagaatgta tttcagttcg
     9121 ccacgcttac gcgccacgtt gtagggcgtt aaccccgctc gggcgataaa cggttttagc
     9181 gccgcaaaaa cgggcgcaaa tgaggcagga taaagcgggc agtcacaaag gtcttccggt
     9241 gtgccgtcgc gatgcagcat accgagcagt ggtttttcaa cgctaccact caccaccatt
     9301 ttggctttat tacgaaaccc ttgttccggg cctgacaccg gcgcgcacca ttcctcaacc
     9361 ggaaagtcgg cgagcagatt tttaagatcg gcggttttag cggagagttg ctctggaatc
     9421 ggctgcgtta tccactgaca ggaacgacag cgacccgcgt cgtaaagtgc gcactgcata
     9481 cattgacctt cacatcatca ggggcgacga ttatacacat tattgcaact ggaagaaccg
     9541 ccgactggtc gagggaacga acagcagcat caggatgagc atatccggca gcttctgcag
     9601 catcaggcta tggaagattt cacgttttga ttcaccggga atgctgaaca gctccggata
     9661 accgtacccc agcgaagccg cccacaagta acttgcggca gtgatttgtg tcagcagata
     9721 cagccagcgc gcccagcgac gccctttcac cagtgaaaac gcacaccaga tctcaatgaa
     9781 aaccagcacc agactgctta aaaagaccag cgttaaactc caggtttgta cgctgcgatg
     9841 aatgaactcg ccaataccgc gcacgcccag cgtattgaaa atcatcagca cgtcgaggcc
     9901 acggatcata ataatggcga gcgccgccac ctgcacaagc gcaggcacat tcaggcgagc
     9961 atgagatgaa gatgttttct taaaaaatcc caacgtttcg tcttccatga aaacaatgcc
    10021 gcgacatacg cggcattatg tagccaggtt ggcaaatttt agtgtcttca gccacgtctt
    10081 gcacactgga tgtcgcgtat ccgctgtttt tctgcgcgag ccatcagata ccaggcgata
    10141 aatccgacaa ttccgaccgc gccgaggatc aatgttgcta gggcgttgat ttccggattc
    10201 acccccatcc gcacgctgga aaagaccagc atcggtaacg tggtggctcc cggcccggat
    10261 acaaagctgg cgatcaccag atcatcaagc gacaaagtaa aagccagtaa ccagccagaa
    10321 atgaccgcgg gcatgatcat cggtagcgta atgacgaaaa acactttcag cggcgtcgca
    10381 ccgagatcca tcgctgcttc ttctatcgag cgatccagtt cccgaagacg cgacgaaata
    10441 acgactgcca cataagccgt acagaaagtg acatgcgcca gccagatggt aagcataccg
    10501 cggtccgcag gccagccaat ggcatgagca agcgcgacga ataataacaa cagcgacaag
    10561 cccgtgatga catctggcat taccagcggc gcggtgatca taaaggcaaa gccatttgat
    10621 ccgcgaaacc tgccaaagcg caccagcacc accgccgcaa tagttccgag gatcgccgcc
    10681 gccgtcgccg cacaggccgc aattgtcaga cttaaaccaa ccgcactcat catcgcgtca
    10741 tcgcgcaata attcgccata ccagcgcgtt gaccagccgg cccacaccgt caccagcttc
    10801 gagctgttaa acgaatagat aaccagcatc agcattggcg cgtagagaaa ggtgaagccc
    10861 aacagcaaaa tcacaatccg ccagggcgaa cgaactaccg gtaaattatt catccgtgtt
    10921 ctcccacgct tttttgctgg tgtttgtgaa accacattat cggcacaatt agcagcagca
    10981 acatgatgat cgctaccgcc gaggccaccg gccagtcgcg gttattgaaa aactcttgcc
    11041 atagcacgcg cccgatcatg atgctgtccg ggccaccgag cagttccggg atcacaaact
    11101 cgcccacagc cgggataaac accagcatcg atccggcaat aatcccacct ttagtcagcg
    11161 gcacgatcac agtaaagaac gttttcagcg gtcgtgcacc gagatccagc gctgcttcca
    11221 ccagcgaata atcaatacga atcaacgcgg tataaatcgg cagcaccata aacggcacgt
    11281 aggcgtaaac aatgccaata taaactgcca gattggtatg aagaatggtc agcggttgat
    11341 cgataacccc cagccacagc agaaaattat tcagcacacc gttgtttttt aatattccca
    11401 tccaggcgta gacacgaatc agaaacgagg tccacgacgg caggatcacc agtagtaata
    11461 agatattacg ggtcgaaggc ttactgtgcg ccaccgccca cgccagcgga tagccgatca
    11521 gtaaacagca aaatgtcgaa atcgccgcca cctgtaacga ctggagataa gcatcgaaat
    11581 agagcggatc gccggtcagt tgcaggaaat taccgaaatt aagggtgatg gacagttgcc
    11641 cgtcagccca ctccatcagt tcggtataag gtggaatagc gcgcgccatc tccgccaggc
    11701 ttattttaaa gacaatcaga aatggcagca gaaacagcaa gatcaaccag atatacggca
    11761 acgcaatgac cagtttgcgc ccatgcttca tttgcaactg cgacaaccac agcttaaatc
    11821 cgcccggcgg tttcgactgg gcagcaggtt caagtgtact catctttgct ccttaaaccg
    11881 tcagcaccac acagctgtcc acttcccagc acaaacgcac ttcgtcgccc caggtcggta
    11941 accctttacg atgacgatgg gcgttttgta gctgggcgct aatcatctgc ccacttttca
    12001 gacgaacgtg atacaccgaa agatcgccga gataggcaat gtgtatcacc tcccccaccg
    12061 cgaagttaca accattggcg ggcggctctt cgcaaagcat gattttttcc ggacgcagcg
    12121 ctacgtgtac cggcacgtta tcgaccactg aagcatccgc atcgactttc agtggatgca
    12181 ccagccccgg cgaataaagc accagaccat cttcctgacg ctctttgagt acaccttcaa
    12241 agacattaac cgagccaata aattcggcgc tatagcgggt agtcggatgc tcgtagatct
    12301 cttccggttc gccaatctgg acaaatttcc cacgattcat aatggcgata cgccccgcca
    12361 tggtcatcgc ctcttcctga tcgtgggtga ccatcacaca agtcacaccg acgcgttcca
    12421 gaatatccac cacttcaagc tgcatccggt cacgcagctt tttatccagc gcgcccatcg
    12481 gctcgtcgag cagtaatagt ttcgggcgct tggcaaggct tcgggccagt gccacacgct
    12541 gtcgctgacc accggaaagc tgatgcggtt tgcgtttggc gaactcctgc atatgcacaa
    12601 ggccgagcat ctcattgacc cggctggcaa tttccgcttt cggtagtttg tcctgtttca
    12661 ggccaaaagc gatgttctgt tccacggtca tatgcggaaa cagcgcgtaa gactgaaaca
    12721 tcatattgat ggggcgcagg taaggcggca cctgtgacaa atcgacgcca tcaagcataa
    12781 tctgcccggc tgaaggttgt tcgaaacctg ccagcatacg cagcagcgtg gacttgccac
    12841 agccggatgc gcccagcagc gcaaagattt cacctttgta gatggtcagg ctgacatcat
    12901 caaccgcatg ctgaccatcg taggatttgg tcagattgcg gatttctaat agcggggtca
    12961 gcgccttacg ggttttcgcc tgggggcgag ggatagcgtc attcacggct gctctccggc
    13021 attaaatgtg tgcaagcgca ccatgttggt gcgaattgcc ggagggagtg gcgctcccgc
    13081 ccggcatcta aggctgcgga ttattttccg ctcttcactt tggtccacgc gcgggtgcgc
    13141 acacggtcga ttttcggagc ctgtactttc agagtgaaca acttcgcgcg gacatccgca
    13201 ggcggataaa tacccgggtt atcacggact tccgcactca ccagcggcgt ggctgcttta
    13261 ttggcgttag catagaacac atggtcagag atatgtgcca ccacatcagg acgcagcagg
    13321 taattcagga actgataggc ttcgtcttta tttttggcat ccgcaggcat ggcgaataca
    13381 tcaaagaacg ccatcgcccc ttcttttgga atcgagaacg agacattcac gccattcttc
    13441 gcttccttcg cgcggtttga cgcctgccag acatcacctg cccagccgat agcgacgcaa
    13501 atatcgccgt ttgccaggtc gttaatatat tgagatgaat ggaaataacg aatgttcggg
    13561 cgcagcttta acagcagatc ggtagccggt ccggtgtaat catccgcttt agtgctgttg
    13621 ggatctttgc cgagataatt cagcacggta gcaaaaactt cttctggcgc atcgaggaaa
    13681 gatacaccgc agcttttcag tttttccaga ttttcaggct tgaggatcaa gtcccagcta
    13741 tcgacgggcg cgttttcgcc cagcaccgct ttgactttat cgacgttata gccaatcccg
    13801 gtcgtcgccc acatataggg catagcaaat ttattgtcgg gatcgtgttt ggcgaccaac
    13861 ttcagcagtt ccgggtcgag attcttccac tccggcaatt tgcttttatc cagcggctgg
    13921 aaaactcccg ctgtcagctg gcgttcaaga aagcttgctg acggcaccac caggtcaaag
    13981 ccggtgctcc cggccattaa tttgccttcc agtacttcgt tagagtcgaa aacatcgtag
    14041 acgactttaa taccggtttc tttttcaaag ttagccaccg tgtcgggggc gatataatca
    14101 gaccagttat aaatgtggag tgttttttgt tcagccgcga gcgtgccgac agagacggcc
    14161 atcagagcac ccgcaaccag acccgaaagc cattttttat ttaaggcggt catatctctt
    14221 tccttctgaa agttcgttaa caaacgaaca aaagttgtca cactgaaaga tatgcaataa
    14281 acgtgcatat tttttatcgg cgcggtgtaa gaaaagagaa ctctctcccg gccagcactg
    14341 ctcgctatat aaaagcatcg caagaataac tttagccagg gtttgagact atagctcgga
    14401 ttaaaaaacg gtatttaaca aatttttatg cgtatttgct ctatgtgata aagccaaatc
    14461 gacacaagag aagtgaaata atctttaaga aaaggttaaa aacagcagat aattttgcag
    14521 cgcgcagcca ctgcaccagc gactgcgtgc tcgaaaggag ttatcagtgg aggaaatagt
    14581 tttcggtaac gttgttttgc ccttcatcgt cagtcggtaa cagcagttga tgggcattag
    14641 cttcaagaat gaccatcgaa atctgctctt cgctctgacg cacgaaccat gcaaactgct
    14701 cataggttac gccctgcata acggataaag actggcaaac caccagcttt ggcagattat
    14761 catcctgcat atcaagaaat gctttcacgg tcagcgaact ggcattgatg gcagataaat
    14821 ccgccgccag cggcaatacg gctgaaggtc ggacttccgc catggcagaa aacaggatcg
    14881 tgttatcgat cagatcgatt ttggcatcaa agacaccgtc gaaattctgc atatggggca
    14941 gatgcagagc ctgacagtta tcacattcaa aaaaactcat ccccaggtca tcgagccatt
    15001 gacgcagcgt atccagacca ggaacgacca gcgatgtcat aattttgtac aacctctttc
    15061 gataaaaaga ccggcacagc ttacgcaaaa agagcaggca aaaccatgat cagtaatgtg
    15121 attgcgacta accacccgtt ttcaggcaat attctgtcgt agcgtggcgt tcgatccagc
    15181 ggatcatttt acccgcgatg tcgataccgg tggttttttc tattccttcc agccccggcg
    15241 acgcattcac ttccatcacc aacggcccgc gattagcacg cagaatatcc acaccagcaa
    15301 cgtccagcgc catcgttcgc gcggctttta tcgcgatttc acgctcctgt ggtgtgatac
    15361 tggcgacgct tgccgcgccg ccacgatgca aattggaacg aaaatcgccc tctttcgccc
    15421 gccgttcaat cgcagcgacc acttcatcgc caacaaccag acagcggata tcgcaccctt
    15481 gcgcctcttt gatatattcc tgcaccagaa tatgcgcgtt cagaccgcgg aaagcgtcaa
    15541 tcacgctttc cgccgcctga cgcgtctccg ccagcacgac gccaattccc tgcgtgcctt
    15601 caaccaactt gaccaccagc ggcgcaccac cgaccatgtc gattaaatcg ctggtatcat
    15661 ccggcgaatg cgcaatgccc gtgacaggca ggtcgatgcc ctgacgcgcc agcagttgca
    15721 tggaacgcaa tttgtcacgc gcccgggcaa tggcgaccga ctcattgagc ggatagctcc
    15781 ccagcatctc gaactggcgc agtgccgccg tcccataaaa ggtaatggcg gtgccaatac
    15841 gcgggatcac tgcgtcaaaa tggggtaact tgcggccttt gtagtgaata gaagacgccg
    15901 caggatttat gttcatgtag caagaaagcg gatcaagaat ttcaaccagg tgaccgcgct
    15961 gtatagcggc ttcacgcagc cgcttacacg aatagagcgt tccatcccgg gacaatatgg
    16021 caattttcac cctgcacctc tctgtcagac ctggtcaaaa gcccgcgtat catacatcgg
    16081 cgtggcggtt ttagcgcgtc gcccaaccct gtttgtgcaa ataatccaga ataaatgggc
    16141 ggctttcttt aatgattgtt cggcggatat gatcgctcca ggtatcccgg cgattattgc
    16201 tgccacgggt gaggtaatat tcagccagtt gttcgtcata ctgcgccagt gcgtctttat
    16261 ccagcggttg atagctgttt tcatgcacca aaatggagga cggtaaacgc ggcttaagat
    16321 ccggattatc cgcaggccag ccaaggcaca gcccaaacag cggcagaaca tgctgcggta
    16381 atttcagcag tttcgtcacc gcttcaatat tattgcgcag gccgccgata tataccccgc
    16441 ccaatcccag cgattccgct gcggttaatg cattctgcgc catcattgcc gtatcaacga
    16501 caccgagcag caattgttcc gccaggccaa gctgagcatc cggacagatc tgtaaatggc
    16561 ggttaaagtc ggcacagaac acccagaact ccgccgcttg cgctacgtgt ttttgcccgc
    16621 cggtcagcgt caccagttct tcacgtaacg ctttgtcggt aatgcgaata atgctactgc
    16681 actgcaaaaa actggaactg gacgtcgcac gggcgctgtt aataatcgcc tcacgctgcg
    16741 cctcggaaat gggttcatca gtgaaatggc gaatggagcg atggccacaa gtaagttcaa
    16801 tggttggcgt cattatcttt ttctctttct gaacgtgaat actgcggtgg acggttcatc
    16861 agctgtgggg caagacgttt tgccacctga agaataacca ccaccgcagc gggaagcatg
    16921 agcaaaacac cgagaaaaat catcagaatc tgcacttctg gccgagaaaa tggctcaggc
    16981 agcgacaggg agtcgcttac cgacagcagc gccaccgcca gcaacatcat tccgataaat
    17041 tccagtatca acacgctttt aggcaattta ccgatcgcgc gcatacgctt ccctctgcaa
    17101 agtgaacctt cagtctaaaa cttttcactg tattgtgttt aacagttata gcttttagca
    17161 attaatgcaa caggttaaac ctactttcag cgaatacatt ttagcgtgat cattacaggc
    17221 ataaatctat gaggagagaa ataatgcaaa ccgttatttt tggtcgtccg ggttgccctt
    17281 actgtgtgcg tgcaaaagat ctggctgaga aattgagcaa tgaacgcgat gattttcagt
    17341 atcagtatgt agatattcgt gcggaaggga tcactaaaga agatctacaa caaaaggcag
    17401 gtaaacccgt agaaaccgtg ccgcagattt ttgtcgatca gcaacatatc ggcggctata
    17461 ccgattttgc tgcatgggtg aaagagaatc tggacgcctg atcgtctgac aagccctcgc
    17521 gttgagggct ttactgattt tttctgtgct gtggtttaaa caagctactg ataaataaga
    17581 aacacagtgc ccccagcgca caccagaaca ccgcgcttag taaccatgcc agctcttgcc
    17641 agaatgagcg ggtcggtgaa aaaaacagcc gcataataag cattgaacag ggtgccgcca
    17701 gcattgcgcc aaatagaggt ttcaggactt ctctacgctg tgaaaagaaa ctggcaactg
    17761 ctccaggaag aatgaaaaac agcaagccga tttcaggatg cccggcagcc cgaaaagcgc
    17821 ctttcatgtg cgtcgccaga aaaaggcaca ccacaatgaa gaggacaaaa cagcagattg
    17881 cccccgccca acgttgttta tgtttcactc gttcctcctg acactgcgtc tatcgaacac
    17941 atttttcgcc agtgtggcgt tcagtaagat aaagccgctt cgcattccat gctaatatag
    18001 gccaacgcaa ttcatatagc cgttgatacc taatgtgatt acactagtaa aatatattgt
    18061 tactttacta tcgtttaggt gcgctgaatg aatctgcgcc ctgaattctg gtaaaaaaca
    18121 ttatcataaa ttaccatttc tttcaacagc
//
LOCUS       DBFIFI010000080        18110 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.080, whole genome shotgun sequence.
ACCESSION   DBFIFI010000080 DBFIFI010000000
VERSION     DBFIFI010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18110
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.080"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(174..1766)
                     /gene="lsrK"
                     /locus_tag="N7B39_002602"
     CDS             complement(174..1766)
                     /gene="lsrK"
                     /locus_tag="N7B39_002602"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310145.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071518 - autoinducer-2 kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HGU2422557.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIAPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTIRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKVGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSTIHPSSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPDPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAPGL"
     gene            complement(1845..2798)
                     /gene="lsrR"
                     /locus_tag="N7B39_002603"
     CDS             complement(1845..2798)
                     /gene="lsrR"
                     /locus_tag="N7B39_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HGU2422558.1"
                     /translation="MTINDSVISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            3047..4582
                     /gene="lsrA"
                     /locus_tag="N7B39_002604"
     CDS             3047..4582
                     /gene="lsrA"
                     /locus_tag="N7B39_002604"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HGU2422559.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITSADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFTRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAAGTSVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNDKEINRLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLELYRRALNI
                     KYNQPEQAARTLSGGNQQKILIAKCLEASPQVLIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     AAC"
     gene            4576..5604
                     /gene="lsrC"
                     /locus_tag="N7B39_002605"
     CDS             4576..5604
                     /gene="lsrC"
                     /locus_tag="N7B39_002605"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HGU2422560.1"
                     /translation="MLKFIQNNREITALLAVLLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALELNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5604..6596
                     /gene="lsrD"
                     /locus_tag="N7B39_002606"
     CDS             5604..6596
                     /gene="lsrD"
                     /locus_tag="N7B39_002606"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HGU2422561.1"
                     /translation="MRIRYGWELALAALLVIEIVSFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGAIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6608..7630
                     /gene="lsrB"
                     /locus_tag="N7B39_002607"
     CDS             6608..7630
                     /gene="lsrB"
                     /locus_tag="N7B39_002607"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HGU2422562.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELGVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            7657..8532
                     /gene="lsrF"
                     /locus_tag="N7B39_002608"
     CDS             7657..8532
                     /gene="lsrF"
                     /locus_tag="N7B39_002608"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HGU2422563.1"
                     /translation="MADLDDIKDGKDFRTDQPQKNIPFTLKGCGALDWGMQSRLSRIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGTQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            8556..8846
                     /gene="lsrG"
                     /locus_tag="N7B39_002609"
     CDS             8556..8846
                     /gene="lsrG"
                     /locus_tag="N7B39_002609"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HGU2422564.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            8903..9661
                     /gene="tam"
                     /locus_tag="N7B39_002610"
     CDS             8903..9661
                     /gene="tam"
                     /locus_tag="N7B39_002610"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HGU2422565.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENVDYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGRESLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLKEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(9665..10579)
                     /locus_tag="N7B39_002611"
     CDS             complement(9665..10579)
                     /locus_tag="N7B39_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HGU2422566.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQVEVLAHYLKTEDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKVLPDRHYQLT"
     gene            complement(10776..12227)
                     /gene="uxaB"
                     /locus_tag="N7B39_002612"
     CDS             complement(10776..12227)
                     /gene="uxaB"
                     /locus_tag="N7B39_002612"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HGU2422567.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(12454..13872)
                     /locus_tag="N7B39_002613"
     CDS             complement(12454..13872)
                     /locus_tag="N7B39_002613"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HGU2422568.1"
                     /translation="MHVHPISTFRVFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLAINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPQMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(14011..14370)
                     /locus_tag="N7B39_002614"
     CDS             complement(14011..14370)
                     /locus_tag="N7B39_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HGU2422569.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPHGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(14370..15296)
                     /gene="glsB"
                     /locus_tag="N7B39_002615"
     CDS             complement(14370..15296)
                     /gene="glsB"
                     /locus_tag="N7B39_002615"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HGU2422570.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(15360..16748)
                     /gene="sad"
                     /locus_tag="N7B39_002616"
     CDS             complement(15360..16748)
                     /gene="sad"
                     /locus_tag="N7B39_002616"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HGU2422571.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGADDIENALQLAAAGFRD
                     WRETNIDYRAEKLRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVTQMIKDSRIAAITVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMDDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITIAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            16849..17730
                     /locus_tag="N7B39_002617"
     CDS             16849..17730
                     /locus_tag="N7B39_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGU2422572.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAIVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNRRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWCWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSTKQGYQ"
     gene            17808..>18110
                     /gene="yneK"
                     /locus_tag="N7B39_002618"
     CDS             17808..>18110
                     /gene="yneK"
                     /locus_tag="N7B39_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HGU2422573.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTIICNE
                     EWRTSLNINSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPARNGRHKEN"
BASE COUNT         4556 a         4602 c         4505 g         4447 t
ORIGIN      
        1 ttatgcttaa tgctttaaga aaaatagcaa tttccccttg aatatagtac tggtgatgaa
       61 acgatgaatc tgcaagctgg catttaacaa gccagctcta aaagaaggga aataagaata
      121 actatactca aaaactaaca gccacggtca tcatgatgtg gctgtcaatg aaactataac
      181 ccaggcgctt tccataacga cgtcgtcagt ccatgatcaa ccagccccag ctgatcctga
      241 taaactgcct gccacttatc gcgtgaatcc tgataaagtt catgcttttc cgggtctggt
      301 gtgtgcgtcc gttcccagcg aaccaggcgt tctccggttt ctgccattga tgaaaaaatt
      361 ccggcaccga cgccagctgc aatggcacat cctaatgcag tggcttcttt gaccaccgga
      421 atattgacgg gtaatcccga gacatcagcg agaatttgac tccataattt cccttttgaa
      481 cctccgcctg caaagactaa cgatgaagga tgaatagtcg aaaaatcagc aatttgctgc
      541 aagttacacg ctgatacaat cgccgcattt tcttccagcg cacggaacaa tgtcgctttg
      601 ttacatttat ccggatcaat ggacaaatta ataaaggaag gcgcagcgtg ataccaggtt
      661 ttaaagcgca ttctgtcgga gaagatcggc attacgcccc acgacccagg cggcacccga
      721 ctggccatct cttccagcag cgtataggtg tcgatgccta aacgttcggc aatcagtttt
      781 tcttcggcac agaaagcatc gcggaaccag cgcatggtga gtccggtaaa aaagcttata
      841 gattcagctt gtaccatgcc aggaataaca tgaggattaa cgcgcacgtt catttctggg
      901 tctgtcaccg gcgcggctaa atttacaact tgctgccaga atgtgccgcc aagaaccgcg
      961 gtttgtgccg gacgcacaac gcctaacccc aggcaaccaa gctgcacgtc gcctcctcca
     1021 acgaccaccg gagtgcccac cttcagaccg cagagttccg ccgcttgtga acttaccacg
     1081 cccagcaatg tgccggtttc tttgacagga gaaagaatat cggcacgcag gccagccata
     1141 tccagcaatg caggtttcca gtcacggatg gttagatcaa gaagtcccgt ggtgccagcg
     1201 ttagagggat ccaccgcaag ttcgccgctg agcatatagg ccagccagtc gctgatcatg
     1261 gtgatggttg atgcctgacg gtaaatatcg gaacgatggt gcgccagcca aagtaatctg
     1321 gggatggcac ttaaagccag tgtttgtccg gtcgcgcgat aaacttcgtt ttcaaaggta
     1381 ttgttgtgca gttctttaag ttcgctaact tcgcgcgccg ctctggcatc cacattggcg
     1441 caggcccaga tcggtgttcc ttcattgtta tataaaacaa tgccttcacg catcgaacat
     1501 gccgaaacgg cagcgatata ctccggggct atgccggcgt tgtgcagcgc ctggcgcata
     1561 cactcacacg ccagttgcca gtttttgttg agatcaaatt ccatagaacc aggaacgtcc
     1621 ggtactgcca gatgccgcca ctccgcctgt cccactgcta tttgattgcc ttccaggtcg
     1681 aatatcacag cccgaatact tccggtgcct gcatccagcg ccatcaggta gtactttgat
     1741 tctgaagggg taaagagtcg agccattaga ttatcctcgg cttatgcgtt ctgtctgttc
     1801 ctctatacgt tctccatcat tcccggtaat aaggtcatgc aaatttaact acgtaaaatc
     1861 gccgctgctg tgtcctgatc ggtaaccagt gcgttgatat aaccgccttt cattgcagcg
     1921 gcaattgctt cggctttatt ttctccccct gccacgccaa cccggacggg tatggtcttc
     1981 agcgcgctta aaggtaagcc aatcagctcg ttatgtattt taatgtccgt gacaacgtca
     2041 ccttttgcat caaaaaagta gcctaaaatg tcgccaaccg ccccttttcg gccaatcatt
     2101 aactgttcgc cctggctgat ataaccggag cgaatgatcg tcgcgtcgtc ttgttgacta
     2161 acggccccaa tgccgacaat cgccacatcc gctgcttgcg cggctaacag aacatctttg
     2221 acacaatttt cattttttag cgtacgggca atgtcagcgg aggatgcccg caacggagcc
     2281 ggaataatat tcacactgca cgccgcatta agctgtccga tacccgtcat ataagaaccg
     2341 acgccaccgg agagcgtgac caggcgaatt tgctgtgacg aaataaaacc acttaagcgt
     2401 tgcagcgtat tcatggttgc ctcgccaaaa ccaatcgcca gcatctgttg tggttgaagt
     2461 aaactcatca acatatgcgc cgcgcctatc cccagtcgcc cgccgacatc agcatccgca
     2521 agcccaggga tcacccggac atgttgcagc gaaaactgac gacgtaattg tgtttcatat
     2581 tccagacagc cttcaaagcg agaattaatc tgcacgcgaa tgatgccgga ctgatgccct
     2641 ttctccagta atcgcgacac tttcaaacgg gtcaggccga gacgatcgct gatctcactc
     2701 tgggtcagcc cgtcgtggta gtaaaaccac gcgatccgcg cgacctgttc ttcttcacac
     2761 attccctgtt ctgaaattac cgaatcgttg attgtcataa ttcattcttc actttgaaca
     2821 tatttaaatc tttaatgcaa ttgttcagtt cttgcgcatt tatatctgtg atggcaacca
     2881 cagtttgact ctacgagcat gaacaaacgc aaccgtgaaa atcaaaatag tataaattgt
     2941 gatctattcg tcggaaatat gtgcaatgtc cacctaaggt tatgaacaaa ttaaaagcag
     3001 aaatacattt gttcaaaact cacctgcaaa actgaacggg ggaaatatgc aaacgagtga
     3061 tacccgcgcg ttaccgctac tttgcgcccg ctcggtttat aaacagtatt caggggtcaa
     3121 tgtcctgaaa ggcatcgatt ttacgttgca tcagggggag gtccacgccc tgctcggcgg
     3181 caatggtgcc ggtaaatcga cgttaatgaa gattattgcc ggtattacct ctgctgatag
     3241 cggtacgctg gagattgggg gcaacaacta cgccagatta acgccagttc atgctcatca
     3301 gctgggtatt tatctcgttc cccaggaacc gctgcttttc ccaagcctgt cgataaaaga
     3361 aaacatcctg tttgggctgg caaaaaaaca gctctccatg cagaaaatga agaacttgct
     3421 ggcggcgctg ggctgccagt ttgatctgca tagtctggca ggatcgctgg atgtcgccga
     3481 tcgccaaatg gtggaaatcc tccgcgggct gatgcgcgac tcgcggattc tgatcctcga
     3541 tgaacctacc gcctcgctta cccctgcgga aaccgaacgc ttgtttactc gcttgcaaga
     3601 gctgcttgct actggcgtgg gtattgtttt tatctcgcat aagctgccgg aaattcgcca
     3661 gattgccgat cggattagcg tgatgcgcga cggaaccatc gccttaagcg gcaaaaccag
     3721 cgaactgtct accgacgaca ttattcaggc catcacccca gcggtacggg aaaaatcgct
     3781 ctctgccagc caaaaattat ggctggagtt acctggcaac cgcccacaac atgccgcagg
     3841 aacgtcggtg ctgacactgg aaaatctgac tggcgaaggt ttcaggaatg tcagcctgac
     3901 gctaaatgcc ggagaaattc tgggtctggc tgggctggtg ggtgccggac gcacagaact
     3961 ggccgagacg ctctatggtc tacgtacttt gcgtggcgga cgcattatgc tgaatgataa
     4021 agagatcaat agattatcca ctggagaacg tttactgcgc ggtctggttt atctgccgga
     4081 agatcgccag tcatccggac tgaatctcga tgcttcgctg gcctggaacg tctgcgccct
     4141 tactcataac cttcgtggat tctgggcgaa aaccgcgaaa gataatgcca ccctggaact
     4201 ttatcgtcgg gcgctgaata ttaaatataa ccaaccggaa caagctgcac ggacattatc
     4261 cggtggtaac cagcaaaaaa tcctgattgc caaatgcctg gaagcctcgc cgcaagtatt
     4321 gattgtcgat gagccgacgc gcggcgtgga tgtctcggcg cgtaatgata tctaccaact
     4381 gttgcgcagc atcgccgcac agaatgtggc tgtgctgctt atctcctctg atctggaaga
     4441 gatcgaactg atggcagatc gtgtgtatgt gatgcatcag ggcgaaatta cccactctgc
     4501 actgaccggg cgcgatatta atgtcgagac tattatgcgc gttgccttcg gcgatagtca
     4561 gcgtcaggag gcggcatgct gaagtttatt cagaacaacc gtgaaatcac ggcactgctg
     4621 gcggtgctgc tgctgtttgt attacccggt tttctcgacc gccagtattt aagtgtgcaa
     4681 acgctgacca tggtttatag cagcgcgcaa atcctgatcc tgctggcaat gggcgcgacg
     4741 ctggtaatgc ttacgcgcaa tattgatgtt tcagtgggtt cgattaccgg aatgtgcgcg
     4801 gtgctgttgg ggatgttact gaacgcagga tattcactac ctgttgcctg tgtcgcgact
     4861 ttactgcttg gtttgctcgc gggatttttc aacggtgtcc tggtcgcgtg gctaaagatc
     4921 cctgccattg ttgccaccct tggcacgtta gggttgtaca gaggcatcat gttgctgtgg
     4981 actggcggta aatggattga agggttaccc gccgaactga aacagctctc cgccccgctg
     5041 ctgcttggcg tttcagcaat tggttggtta acgataattc tggtggcatt tatggcctgg
     5101 ctgctggcga agacagcgtt tggacgcagt ttttatgcca cgggcgataa tttacagggc
     5161 gctcgtcaac tgggcgttcg tacggaagcc attcgcattg tggcattttc gttgaacggc
     5221 tgcatggcgg cactggcggg aattgtgttt gcttcgcaga ttggttttat ccccaaccag
     5281 accggtaccg ggctggagat gaaagcaatt gcagcctgcg tgctgggcgg cattagtttg
     5341 ctcggcggtt ccggtgcgat catcggtgcg gtactcggcg catggttcct gacgcagatc
     5401 gatagcgtac tggtgctgtt gcgcattccg gcatggtgga atgattttat cgcgggtctg
     5461 gttctgctgg cggtgctggt gtttgatgga cgcctgcgtt gtgcgctgga acttaatctg
     5521 cggcggcaaa aatatgcccg ctttatgacg ccaccgccat ccgttaaacc cgcttcgtca
     5581 ggtaaaaaac gggaggctgc ataatgcgta ttcgctacgg ttgggaactg gctcttgccg
     5641 cactgcttgt tattgagatt gtctcatttg gtgcaattaa cccgcggatg ttagatctca
     5701 atatgttgct gttcagcacc agtgacttta tctgcattgg cattgtcgcc ctaccgctga
     5761 cgatggtgat tgtcagtggc gggatcgata tttcgtttgg ttcgaccatc ggcctctgcg
     5821 ccattgcatt gggcgtactg tttcaaagtg gtgtgccgat gccgctggcg attctcctga
     5881 ccttactgct cggcgcattg tgcgggctga tcaacgccgg attaattatc tataccaaag
     5941 ttaacccgct ggtgattacg cttggcacgc tgtatctgtt tgccggaagc gccctgctgc
     6001 tttccgggat ggccggggcg acggggtacg aaggtattgg tggattcccg atggcgttta
     6061 cagatttcgc taacctggat gtgttggggc tccccgttcc gctgattatc ttcctgatat
     6121 gtctcctcgt tttctggctc tggctgcata aaacccatgc cggacgtaac gtgtttttga
     6181 ttgggcaaag cccgcgcgtg gcgctttata gcgcgattcc agttaaccgt accttatgtg
     6241 cgctctatgc catgacgggg ctggcgtctg cggtcgccgc tgtgctgctg gtgtcgtatt
     6301 ttggttcagc acgttccgat ctcggtgcgt cgtttctgat gcccgccatc accgccgtgg
     6361 tgcttggcgg tgccaatatt tatggtggtt ccggtgccat tatcggcacc gccattgcgg
     6421 ttttattagt gggatatttg caacaaggtt tgcaaatggc aggagtgcca aatcaggtgt
     6481 ccagcgccct ttccggtgcg ctacttatcg tcgttgtcgt aggtcgttcc gttagcctgc
     6541 atcgccagca aattaaagag tggctggcgc gtcgggccaa taacccattg ccataaagga
     6601 tatcttcatg acacttcatc gctttaagaa aatcgcctta cttagcgctc ttggcattgc
     6661 cgcaatctct atgaatgtgc aggccgcaga gcgtattgca tttattccca aactggttgg
     6721 cgtgggattt tttaccagcg gtggcaacgg cgcacaacaa gcgggtaaag agctgggcgt
     6781 tgatgtgacc tacgacgggc cgacagaacc cagtgtttct ggtcaggtac agttgattaa
     6841 taacttcgtc aatcaaggtt ataacgccat tatcgtttct gcggtttcgc ctgatggctt
     6901 gtgtccggca ctgaaacgcg ccatgcaacg tggtgtgaga gtgctgacct gggactctga
     6961 tactaaaccg gagtgccgct cttactacat taatcaggga actcccgccc agttgggagg
     7021 tatgttggtg gatatggcgg cgcgtcaggt gaataaagac aaagccaaag tcgcgttttt
     7081 ctactcaagc cccaccgtta cggaccaaaa ccagtgggtg aaagaagcga aagcgaaaat
     7141 cgccaaagag catcctggct gggaaattgt cactacgcag tttggctata acgatgccac
     7201 taaatcatta caaaccgcag aaggaatatt aaaagcgtat agcgatctcg acgccattat
     7261 cgcccccgat gccaatgccc tgcccgctgc cgcacaagcc gcagaaaacc tgaaaaatga
     7321 caaagttgcg attgtcggat tcagtacgcc aaacgtgatg cgtccatatg tggaacgcgg
     7381 cacggtgaaa gaatttggcc tgtgggatgt ggttcagcaa ggcaaaattt cagtttatgt
     7441 cgcggatgca ttattgaaaa aaggatcaat gaaaacgggc gacaagctgg atattcaggg
     7501 cgtaggtcag gtggaagtct cgccaaacag cgtccagggc tatgactacg aagcggatgg
     7561 taatggcatc gtactgttac cggagcgcgt gatattcaac aaagagaata tcggcaaata
     7621 cgatttctga tgtgcattac ttaaccggag taagttatgg cagatttaga cgatattaaa
     7681 gatggtaaag attttcgtac cgatcaaccg caaaaaaata tcccttttac cctgaaaggt
     7741 tgcggtgcgc tggattgggg aatgcagtca cgcttatcgc ggatatttaa tccgaaaacg
     7801 ggtaaaaccg tgatgctggc ttttgaccat ggttattttc agggaccaac taccggactt
     7861 gaacgcattg atataaatat cgccccgctg tttgaacatg ccgatgtatt aatgtgtacg
     7921 cgcgggattt tgcgcagcgt agttcccccg gcgaccaata agccggtggt actgcgggcg
     7981 tcaggtgcga actctatcct ggcggaatta agcaatgaag ccgtggcgtt atcgatggat
     8041 gacgccgtgc gcctgaacag ttgcgcggtg gcggcgcagg tttatatcgg cagcgaatat
     8101 gaacatcagt cgatcaaaaa tattattcag ctggtcgatg ccggaatgaa agtgggaatg
     8161 ccgaccatgg ccgtgactgg cgtgggcaaa gacatggtgc gcgatcagcg ttatttctcg
     8221 ctcgcgactc gaatcgccgc tgaaatgggg acgcaaatta tcaaaaccta ttatgtcgaa
     8281 aaaggttttg aacggattgt tgccggatgt ccggtaccca ttgttattgc tggcggtaaa
     8341 aaattaccgg agcgcgaggc gctggaaatg tgctggcagg ctatcgatca gggcgcttct
     8401 ggtgtggata tggggcgtaa tattttccag tctgaccatc cggtggcgat gatgaaagcc
     8461 gtacaggcgg tggttcacca taacgaaacg gctgatcggg catatgaact ctatctgagt
     8521 gaaaaacagt aactgcggat ttaaggagaa gaattatgca cgtcacactg gttgaaatta
     8581 acgttcatga agacaaggtt gacgagttta tcgaagtttt tcgccagaac cacctgggct
     8641 ctgtacagga agaaggcaat ttgcgcttcg atgtcttaca ggacccggaa gtgaattcgc
     8701 gcttttatat ctacgaagcc tataaagatg aagacgcagt ggcgttccat aaaaccacgc
     8761 cccactacaa aacctgtgtc gcgaaactgg aatctttaat gaccgggccg cgtaaaaaac
     8821 gtctgttcaa tgggttgatg ccgtgaggcg aatttatcag ttttatctac aattggggta
     8881 acgcgctgac gggagtaaat aaatgtctga ctggaacccc tctttatatc tgcactttgc
     8941 cgctgaacga tcgcgtccgg cggtggagct gcttgccaga gtgccgctgg agaatgtcga
     9001 ttatgttgcc gatcttggct gtggcccagg taacagcacc gccctgctgc atcaacgttg
     9061 gcctgctgcc aggataacgg gcatcgactc gtctccggcg atgattgctg aagcgcgcag
     9121 tgctttgcca gactgccagt tcgtggaagc agacatccgc aactggcaac cggaacaggc
     9181 actcgatctg atttttgcta atgcctcact gcaatggctg cccgaccact acgaattgtt
     9241 tcctcacttg gtttctttac ttaatccgca gggcgtgctg gcagtacaga tgccagataa
     9301 ctggctggag ccgacccatg tgctcatgcg cgaagttgcc tgggaacaaa actacccgga
     9361 tcgcgggcgt gagtcgttgg ctggtgttca tgcttactac gatattttga gcgaagccgg
     9421 atgtgaggtc gatatctggc gaaccaccta ctatcaccag atgccgtcgc accaggcgat
     9481 tatcgattgg gtgaccgcca ctggattacg tccgtggtta caggatctga ccgagagcga
     9541 acagcagctt tttcttacgc gctaccatca gatgctgaaa gagcagtatc cactgcaaga
     9601 gaacggacag atactgctgg catttccgcg tctgtttatt gttgcccgcc gtacggagta
     9661 aatatcacgt cagttggtaa tggcgatcgg gaagaacttt tgctggaatt tcggcttcat
     9721 cgttcatctg taagaggtca atttcaatag cgttacagat ggcatccagc ggtaaatcat
     9781 tgttttcagt accgaacgga tcttccagtt cttccgccag acaatccagc gaaataaaag
     9841 tataggaaat cagcacagag ataaaaggcg tcatgtaatg caggtccacg accagcgcga
     9901 acggcagcat gatacaaaac agataaacgg tacgatgcaa aatcagggtg taggcaaagg
     9961 gaattggcgt gtaggcaatg cgctcgcagc ctgccagaac cgctgaaata tcgttaagcc
    10021 gatcgttgag gctaataaac aggatatctg aaagttgtcc attgcgtcgc tgaaccgcca
    10081 accactcccc cattattaac aagatgcggt tagctggaga gttcgaagcc agtacacgct
    10141 gaagatcttc agtcttgaga taatgagcca gcacttccac ctgtggctgt ttgcgtaatg
    10201 tcatgcgtaa acagtgggcg aaggcgattt gcagccgggc aaactccctt acacttgccg
    10261 aatccggcaa tgtcgttttt acctcgcgca gtaacgaccg tgaggcaatc atcaactgcc
    10321 cccaaagttt gcgcgcttca acgtaacggg cgtaaccggc attattacga aaaccaagaa
    10381 aaatggcgat ggcgacaccg agaatgctga acggtgcgag ggtgaatttg atgcccagat
    10441 gcgtgtacca gggcagcatg aaaataacag cgatagaaaa gagaaaattg agtagtaagc
    10501 gcgaggatat cttggataat actgagccat gccagacaaa aatacggcgc agccagtgtt
    10561 gttgtggacg aacaatcatg gttatcttca ggcgtggaaa agtcgcccta ttaaacgtga
    10621 ttacgatcac attctcaaga cactacttac aaattaccta caaaattaca acattaacag
    10681 gtgctgcatg tggcacctgt taatgataaa ggcttatagg atgttgtaac taatataatg
    10741 aaataagaaa cccggtcgaa accgggttca gagtattagc acaacggacg tacagcttcg
    10801 cgcatccctt tttcgagaat cgcatccagg tcattagcca cctgctcgac cagaccaggc
    10861 acttgcgtca ggtcctgctc ccagtggtct ttttctgcca acacaatcgc taccagttcc
    10921 tgggtaccga taacacggtc acgatgctga ctccagagtt gctgataacg ttccagccag
    10981 tgtgcatcgt cctgtaccgg atatgtttct ccattacgct caccgcgata gaacgcaatc
    11041 aatgccgcta atgcgaaagt aaggcgcgcc ggaagtgtgc cgtttgcctt ctgccctgcc
    11101 agcagctgcg gcaggatgcg ggtacggaac ttggtcatac cgttgagcgc gatagacagc
    11161 agctgatgct taatgtacgg gttacggaaa cgcccggtga ctgcactggc gaaagattcc
    11221 agttcatcgc gtggcaaatc cagtaccgga ataatttctt cgtagatggc tttttcaacg
    11281 aatgcgcaaa tttcagcatc gttcatcgct tcacctacgg tatccagccc tgcctggaac
    11341 gccaccggca ccaacgcggt gtgtgcaccg ttgagtattg ccactttgcg ttctttatac
    11401 ggtttaatat cgtcaacaat cagcacgttg agcggatatt tgtccagacg cagctcggtc
    11461 gctaaggatt tcggtccctg aatcacaaac aagtaaaagt gttcagcggt gtcgaggaaa
    11521 ccatcgtgat aacccagttc ttcttccagt ttagccactt catcacgcgg ataaccggta
    11581 acgatacggt ccaccagcgt agaacagaag ctgttagcct gatccagcca ttgaataaat
    11641 gcttctggca gtgcccactc ttgcgcatag cgcagcacca gttcacgcaa cgcgtcgcca
    11701 ttgtagtcga tcaactcaca cgggatgatg atccaacctt tatccagcgc accgttgaaa
    11761 tggctaaaac gttcgaacag cagacgggtc agttttgccg gataacttac cgctggcgcg
    11821 tcatcgaatt tatcgcccgc atggtagctg atacccgctt cggtggtgtt agagaaaaca
    11881 aagcgcattt ccgggttgtg cgccagtttc aggaattcat cgtattcact gtagacgctg
    11941 atttcacgat taacagagcg aatcagacgc gcgtcgctga ccgcttcccc cttctcattc
    12001 aggccacgga taatggtggt gtacagacca tcctgcgtgc tcagtgacgg cgggaatgaa
    12061 gtttcaatcg gacgaacaac gaccacacca gaattcagat cggtgtgctc attcaggaga
    12121 tctatttgcc agtcaacaaa ggcgcgcagg aagttacctt caccaaactg aatgatacgt
    12181 tctggatact gtgcaccggg aaaatcgcga cgatttagtg ttttcacaat gggttccctt
    12241 ctgattagtc atacaacctg tttgaattgg tacgacaggt tagcaaactt taatacgacg
    12301 aacccctgtt ttgatcaact cctgatgatt aatgagcagt tttatgagaa aagtgtggcg
    12361 cggatcatgg tttaatcgag gaaatacgcc ttttcctgga tcataaagtg gtagaacaca
    12421 ttgcattcaa atcgcgcgga atgaataaag atgtcagaca acttcctcac cgtaacgcat
    12481 agtgctggta cggttgcgcc catctttctt cgagcgatac agacaggtat cggcttcgac
    12541 catcaattta ttaaagtcat cggtcagcgt tcggtacgat gcgcgaccac taccgacgcc
    12601 aatacttacc gtgagataaa gcgttttttg ttgccaggtg aatggttgca gttcaacgcc
    12661 tttacggatt ttttccgcca ttagcagacc atcgacagga ttcactgacg gcactgcaac
    12721 agcaaattct tcgccgccca tgcgcgccac cagcccctta tcaccgacaa tcttctgaat
    12781 atgccgggca aacacgctta acactttatc gccacattca tgtccatagt tatcgttaat
    12841 acttttgaaa tagtcgatat caagtagcat gacagtcaga tgttgtgtct gtttcagcga
    12901 tggacttttc agcgcctcat aaagaccgga ccgtgagtaa acctgagtca gaaaatcaaa
    12961 gtcggctcgc agcgcaactt gcttcattag cgaattgatc gctgccacgc taaaagaaac
    13021 cataattggg catatcgcca tcgtggcaat accgagacgt gcggagaaca tttgcggaat
    13081 ggagaacggc gaaccgaccg agatatcaat caccgaatta gccaccagta cgatttccac
    13141 cgcaccggtg acaaaggtca acagacatgt tacctgcggc gtatagcgca ctgcacacca
    13201 gattaatgca ggcagcggga acgccagact ccccgctccg ccaatgacta ccgaggcaat
    13261 aaccgacaca ataagcgcga tggcgggcat catctgttct gctttaaagc gcggcagcac
    13321 tccaggaata gccaacgtca gcatacaagg cacgatcaac acgcccgttg agaattgctc
    13381 actgaaccag tccgcaagca aaggccagaa atccagacta tcaatactga ccgaaccaat
    13441 cgccccgaca atagcgcata ataatgcggc aatcagacag taattaaata gccgtaaggc
    13501 acttactggc tcatatttat ttttccccaa acgcttatcc cgcacgacca gtaaggcgac
    13561 ggtaacaata aacatcatat tggagaaatt gatagccagt gaaaccagtc cccattcggt
    13621 ggttatggca tcataaacca gcatcgccac ataactgatc gcatagtaat gcagtcgatt
    13681 aagccagaca tagcgggcaa acacccccgc cattacgcca ttcagcggcc agaaaagaga
    13741 aagttcgtga accagccgta actctgcacc aataaaatag aacagcgtgg ttagcgcaaa
    13801 aatagcgatg ctattacgta gcagatgacc ttcctggaac acacggaaag ttgaaatggg
    13861 gtgtacatgc attaattatc tgtccataca attgcgggtg gcaattatct caacgtaata
    13921 agtaaattct cataaaggat agcatgttcg ctgacagcgg cggcaaataa aggtaccctg
    13981 ggaatgctat tttgtcgttt tccgcaattc tcatggctga ttcatctgta caaccagcca
    14041 gtgatagatt accgccacga tataacgttg ttgttcttcg cttagcgaaa caccatgcgg
    14101 gatgttatgc cacttagcca gacaaccgcg acagcaggtc gcggtggcgt gctgagcgat
    14161 aaacaccgga tgcccgcgca tgggggtttg cttaccgtca ttagccggta atgccggagc
    14221 aagacgttta gcgacaaaat ccgccgcatg ttgttcgatg actggcgcgc ctttctccag
    14281 acaatactga cgctctttca tgcccaaacg aaagcgagat cgaaattttg aacgggataa
    14341 acgcgcgaag agtggatcga gagactgcat taataaaccg aacgccctaa ctgtttcgtc
    14401 aattgttcaa ggacggcgat acccgcaagc gagttacctg catcatccag ttccggactc
    14461 cagactgcga tggccatttc atgcggaaca atcgccacaa taccgccacc aacgccagat
    14521 ttcgccggta gtcccacccg ccaggcaaac tcccccgcgt tctggtacat accactggtc
    14581 gccatcagcg cgttaatttg ccgtgcctgc attggcgtca ccacaggttc atcaatatga
    14641 atagctttcc cctgattagc cagaaagaca aacgtccggg ccagctctac acagctcatt
    14701 ttcagagcgc agtaatgaaa atagttttgc agaacggttg tcacgtcatg atggaaattg
    14761 ccaaacgact tcatcagcca ggcgatagcc gcatttcgcg cggaatgttc aaattcggaa
    14821 cgcgctacca ccgtatcgta ggaaatatca gacacaccgc ttaagccgcg cacgacttcc
    14881 agcatacgtt gccgtggtgc gcttaatcgc ccttgcaaca tatcgcagac caccagcgca
    14941 ccagcattaa taaacggatt acgcggaata ccctgctcca tttccagttg cactaaagaa
    15001 ttgaacggtg atccagacgg atctttgccg acgcgttgcc agatttcctc ttcggagtaa
    15061 tgacgcatgg cgacaacgag actcagcact ttggaaatag actgaatgga aaaacgttct
    15121 tgcgcgtctc cggcctgaaa aagctgtccg tcaacggtac agatagcaat gcccaatcgg
    15181 gaaccgtcta ctgtagccag cgccggaata taatccgcga ctttaccctg accaatgagc
    15241 ggccgcactt gccgcaagat gttttctaaa attgcattat ccatggcgac tgccactttc
    15301 tactcctgga ccgcaggtct gaaaagacct gcgagtatat cagagctgaa tatgtcgcgt
    15361 cagatccggt ctttccacac cgtctggata ttacagaatt cgtgtaagcc gaaatgggaa
    15421 agctcacgac caaagccact ctttttcacg ccaccaaagg ccactcgcgc gtcgctggca
    15481 caataaccat tgataaacac cccaccgcat tccagacgtg ccgccatctg tctggcctgt
    15541 gtttcgtcgg tggtaaaaat ggtcgctgaa aggccgaact cactatcatt agccagttcc
    15601 agcgcatgtt ctgcatcttt cgcaatggtg attgccgcaa cggggccaaa catttcttcc
    15661 cgaaaagcgg tcatttctgg ggtaacattc gccagaaccg ttggcggata gtagttacct
    15721 gccccagcca tcttttcccc gcccagtaac aaacgcgcac cctgcgccag ggttttctcc
    15781 acctgatgat gcagctcatc acgtaaatcg aaacgagcca ttggtccgag agcgttctct
    15841 tcgtcacggg gatcgtccat tttcaaggct gccgcagctg ccacaaaacg ttcggtaaat
    15901 gccgaagcaa ttccctcttc gataataaag cgttttgctg ctgcacaaac ctgtccggta
    15961 ttctgataac gtccggctac cgccgcttta actgccagtt ccagatcggc atcgttaagc
    16021 acaataaatg gatccgaacc gcccagttcc agtacgcatt ttttcagtgc cgctccagcc
    16081 tgtgcaccaa tagccgctcc agcacgaaca cttccggtca ccgtgatagc agcaatgcgc
    16141 gagtctttaa tcatctgagt gacaccgtca ttgtcggcat tcagccagcc atatacgcct
    16201 tgtgggattc ccgcatcttt aaacacctgg gcaatgagct gagcacagcc catcacattc
    16261 ggcgcatgtt taagtaagta tccgttacca gcaagaatga tgggaacagc gccacgcatc
    16321 acctgccata acggaaaatt ccacggcata atcgccagaa tcgtccccaa cggtcgatac
    16381 tcaataactg cctgctgatt ttccaccagc gtaggttccg ccttcagcat tgccggacca
    16441 tgttctgcat accagtcaca caaattcgcc gatttcgcca cttcagcgcg cgcctggttg
    16501 attggtttgc ccatttcgcg ggtgatcatt tgcgccattt cttcgctacg ggcgcgcaga
    16561 gccttaccga tatcacgcag tttttcagca cgataatcta tatttgtctc gcgccagtca
    16621 cgaaagcctg ctgccgccag ctgaagtgcg ttttcgatat catcagcgcc agcccacggc
    16681 aacacagaaa gttgttcacc cgtggcagga tttatcgaaa ttgcatgagt tgccggagta
    16741 atggtcatcg gggtatctcc tttatgagtc atggtatgaa gatacgcaga tttactcttg
    16801 ctttaaaatg aataatatta agccacttat tcacgaatcg agaatattat ggatctgacc
    16861 caactggaga tgttcaacgc ggttgccgaa gctggcagca ttacgcaggc tgcagcaata
    16921 gtgcatcgcg tgccgtcgaa tctcactacc cgtctacgcc agctggaaac agaactgggg
    16981 gtagatctgt ttattcgcga gaatcagcgt ttacgtctat ctcctgccgg gcataacttt
    17041 ttacgctata gccaacaaat tctcgcgtta gtggatgaag cgcggagcgt tgtcgctggc
    17101 gatgagccgc aaggtttatt ttctctgggt tcgctggaaa gcaccgcagc agttcgcatt
    17161 ccggccacgc tggcggaatt caaccgtcgt tatcccaaaa ttcagttttc actttccacc
    17221 ggcccttccg gcactatgct ggaaggtgtg ctggagggaa aacttaatgc ggcgtttatt
    17281 gatggaccca ttaaccatac tgccattgac gggataccgg tataccgcga ggagctgatg
    17341 atcgtcacgc cacaaggtca tgcgccagta atccgtgcca gtcaggttaa tggcagtaac
    17401 atttatgcct tccgcgccaa ttgttcgtat cgtcgccact tcgagagctg gtttcatgct
    17461 gacggtgccg ctccgggaac tatccatgag atggagtctt atcacggaat gttggcctgt
    17521 gtggtcgcag gagcaggtat tgcgcttatt ccgcgctcta tgctggaaag tatgccgggg
    17581 catcaccagg ttgaagcgtg gccgttagct gagcaatggt gttggctaac aacctggctg
    17641 gtctggcgtc gtggtgcgaa aacgcgtccg ctcgaggcat ttattcaact actggatgtg
    17701 cctgactcga caaaacaggg ttaccaatga gctatttttg atagttcttg cggctaatat
    17761 gctctatata gtgatgttcc gatgacttat gactatatgg ggcaaatatg gttacgccag
    17821 taagcatcag caattacata tctcttcctg atgattttcc cgttcgtaat attgcaccgc
    17881 aggtaaaaga agttttaaag gattttattg atgcacttag tacaataata tgtaatgaag
    17941 agtggcgcac gtctttaaac atcaactcag ccacaaaaaa gatatttaat aatcttgaca
    18001 acttatcata tattcagagg acttcttttc ggggtaacga cacgctatac aatgaaaagg
    18061 ttcagtttaa actcacttat cccgccagga atggaagaca caaagaaaat
//
LOCUS       DBFIFI010000081        18085 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.081, whole genome shotgun sequence.
ACCESSION   DBFIFI010000081 DBFIFI010000000
VERSION     DBFIFI010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18085
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.081"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            16..1236
                     /gene="ybdN"
                     /locus_tag="N7B39_002619"
     CDS             16..1236
                     /gene="ybdN"
                     /locus_tag="N7B39_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HGU2422574.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQSLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            1209..1838
                     /gene="ybdM"
                     /locus_tag="N7B39_002620"
     CDS             1209..1838
                     /gene="ybdM"
                     /locus_tag="N7B39_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HGU2422575.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRINAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(1839..2999)
                     /gene="ybdL"
                     /locus_tag="N7B39_002621"
     CDS             complement(1839..2999)
                     /gene="ybdL"
                     /locus_tag="N7B39_002621"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HGU2422576.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     IADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTREHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3108..4196
                     /gene="hcxA"
                     /locus_tag="N7B39_002622"
     CDS             3108..4196
                     /gene="hcxA"
                     /locus_tag="N7B39_002622"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HGU2422577.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGVR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4206..4403)
                     /gene="ybdD"
                     /locus_tag="N7B39_002623"
     CDS             complement(4206..4403)
                     /gene="ybdD"
                     /locus_tag="N7B39_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HGU2422578.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4585..6690)
                     /gene="cstA"
                     /locus_tag="N7B39_002624"
     CDS             complement(4585..6690)
                     /gene="cstA"
                     /locus_tag="N7B39_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HGU2422579.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKEPKPTAKETP
                     YEPMPENVEEIVVQAKGAH"
     gene            complement(6871..7284)
                     /gene="entH"
                     /locus_tag="N7B39_002625"
     CDS             complement(6871..7284)
                     /gene="entH"
                     /locus_tag="N7B39_002625"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HGU2422580.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDI
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7287..8033)
                     /gene="entA"
                     /locus_tag="N7B39_002626"
     CDS             complement(7287..8033)
                     /gene="entA"
                     /locus_tag="N7B39_002626"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HGU2422581.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8033..8890)
                     /gene="entB"
                     /locus_tag="N7B39_002627"
     CDS             complement(8033..8890)
                     /gene="entB"
                     /locus_tag="N7B39_002627"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HGU2422582.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(8904..10514)
                     /gene="entE"
                     /locus_tag="N7B39_002628"
     CDS             complement(8904..10514)
                     /gene="entE"
                     /locus_tag="N7B39_002628"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HGU2422583.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLLNDSGE
                     HNLQDAINHPADGFTATPSPADEVVYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQINVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(10524..11699)
                     /gene="entC"
                     /locus_tag="N7B39_002629"
     CDS             complement(10524..11699)
                     /gene="entC"
                     /locus_tag="N7B39_002629"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HGU2422584.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QASARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            11888..12844
                     /gene="fepB"
                     /locus_tag="N7B39_002630"
     CDS             11888..12844
                     /gene="fepB"
                     /locus_tag="N7B39_002630"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HGU2422585.1"
                     /translation="MRLAPLYRNALLLTGLLFSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSTEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQALLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(12848..14098)
                     /gene="entS"
                     /locus_tag="N7B39_002631"
     CDS             complement(12848..14098)
                     /gene="entS"
                     /locus_tag="N7B39_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HGU2422586.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDG"
     gene            14209..15213
                     /gene="fepD"
                     /locus_tag="N7B39_002632"
     CDS             14209..15213
                     /gene="fepD"
                     /locus_tag="N7B39_002632"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HGU2422587.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15210..16202
                     /gene="fepG"
                     /locus_tag="N7B39_002633"
     CDS             15210..16202
                     /gene="fepG"
                     /locus_tag="N7B39_002633"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HGU2422588.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16199..17014
                     /gene="fepC"
                     /locus_tag="N7B39_002634"
     CDS             16199..17014
                     /gene="fepC"
                     /locus_tag="N7B39_002634"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HGU2422589.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTLGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(17011..>18085)
                     /gene="wzz(fepE)"
                     /locus_tag="N7B39_002635"
     CDS             complement(17011..>18085)
                     /gene="wzz(fepE)"
                     /locus_tag="N7B39_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HGU2422590.1"
                     /translation="SPSNNEIDLLNLISVLWRAKKTVMAVVFAFACAGLLISFILPQK
                     WTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTEAFNLFIKKFQSVSLLEEYLR
                     SSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNASKKKDEPSLYTSWTLSFTAPT
                     SEEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFEKEKLAQDRIKMKNQLDANIQ
                     RLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGADGIERKLEIEKAVTDVAELNG
                     ELRNRQYLVEQLTKANINDVNFTPFKYQLSPSLPVKKDGPGKAIIVILSALIGGMVAC
                     GSVLLRYAMASRKQDAMMADHLV"
BASE COUNT         4247 a         5145 c         4670 g         4023 t
ORIGIN      
        1 agagagcgtc ttcagatgtc tatttataaa attcctcttc cgctcaatat tctggaagct
       61 gcacgagagc gtattacctg gacactcaac accctacccc gcgtttgtgt ctcattttct
      121 ggcggcaaag actctggttt aatgctgcat ctgacagccg aactcgcccg acaaatgggc
      181 aaaaaaatct gcgttttgtt tatcgactgg gaggcgcaat tctcttgcac cattaactat
      241 gttcagtccc tgcgcgagtt gtacaccgat gtcatcgaag agttttactg ggttgcgctc
      301 ccgcttacga cgcaaaattc cctttcacaa taccaacccg aatggcagtg ctgggaacct
      361 gatgtcgaat gggtacgtca gcccccgcaa gatgcgataa ccgatcctga ctttttctgc
      421 ttttaccagc caggcatgac cttcgaacaa tttgtacgtg agtttgccga atggttttca
      481 caaaaacgtc cggcggcgat gatgatcggc atccgtgcgg atgagtccta caaccgtttt
      541 gtcgccatcg ccagtttaaa taaacaacgt tttgccgacg ataaaccctg gactacggcc
      601 gcaccaggcg gtcatagctg gtacatttac cccatttacg actggaaagt ggctgatatc
      661 tggacctggt atgcaaatca tcaaagtctc tgcaacccac tgtataacct aatgtatcag
      721 gcaggcgttc ctctgcgtca tatgcgaatt tgcgaacctt ttggcccgga gcaacgacaa
      781 ggattatggc tctatcacgt tatcgaaccg gatcgctggg ctgctatgtg cgcacgagtc
      841 agcggggtaa aaagtggcgg catttacgcc ggacatgata atcatttcta tgggcatcgg
      901 aaaatcctca agccagaaca tttagactgg caagaatatg cattattgct gctcaatagc
      961 atgccggaaa aaacagctga gcattaccgc aataaaatcg ccatttattt gcactggtat
     1021 cagaaaaaag gcatcgaggt tccacaaacc cagcaagggg acattggcgc gaaagatatc
     1081 ccctcctggc ggcggatatg caaagtttta ctcaataacg attactggtg tcgggcatta
     1141 tcatttagtc ctacgaaagc gaagaactat cagcgttata acgaacggat aaaaggaaaa
     1201 cgtcaggaat gggggatact atgcaacaac gattaacgca ggatttaacg caatttctcg
     1261 ccagcttgcc ggaagatgat cgcatcaacg cgattaatga aatccgcatg gcgatccatc
     1321 aggttagccc ctttcgtgaa gagccagtgg attgcgttct gtgggttaaa aacagccaac
     1381 ttatgcctaa tgattacaac ccaaacaacg tggcaccacc cgaaaaaaaa ctgctgaaaa
     1441 aatccattga aatagatggt tttacccaac ccattgtggt cacgcatacg gataaaaatg
     1501 ccctggaaat cgtggatggt tttcaccgcc atgaaattgg taaaggctca tcatcattaa
     1561 aattacgctt aaaaggttat ttgccggtga cctgtctgga aggtacgcgt aatcaacgca
     1621 tcgccgcgac tattcgtcat aatcgcgctc gtggacgcca tcaaatcacc gccatgtcgg
     1681 aaatcgtccg tgaactcagt cagttgggat gggacgataa taaaattggc aaagagctgg
     1741 gaatggacag cgacgaagtg ctgcgcctga agcaaattaa cggtctgcaa gagctgtttg
     1801 ccgaccgtca atattcccgc gcctggactg tcaaataact acagctggcg cagtcgttca
     1861 gctgctgcca gcaacgtcga ttccttcttg gcaaaacaga gacgaatcag tttatgtggg
     1921 aagggatcgg cgcaaaacac cgatagcgga atcgccgcca cgccgtgctc ccgcgtcagc
     1981 cactggcaaa actcaacatc atccagggta gaaaccgcgc tgtaatccac cagcaaaaag
     2041 tatgtgcctt cgcacggtaa aatctccagc cgactttcat ttaaggcatt taccagaata
     2101 tcgcgcttct ggcgataaaa gtccggtaac gcaagataat gctcaggttc tgcacgtagc
     2161 atatccgcaa ttgccagctg tgccggggta ttcaccgaaa aggtcagata ctgatgtacc
     2221 ttgcgaattt cggcgctgat gggcgctggc gcaacacaat aacccacttt ccagccggtc
     2281 atatgatagg tcttgccaaa cgatgacacc gccaccgccc gctcacgcag ctgcggatgc
     2341 gccagcacac tggcatggcc ctgttgtgaa aagttgatgt gctcgtagac ttcatcgcta
     2401 atgacaaaaa tctcgtgccc ggcgatcgcc tgccacaaag cggcgaaatc agcctgctgc
     2461 cagacagttg cactgggatt atgcggagtg ttgaggatca ccagtctggt gcgctcgctt
     2521 aacaatgcgg caaattcctg ccagtcaacg cgaaaatgcg gtggttgcag tgccatacgc
     2581 ttcactattc ccccagaaag cgcgatggcg ggggcgtaac tgtcatagct gggatcaaaa
     2641 caaatcactt catcgccatt gcgcaccagt gcggtaatcg ccgcgtataa cgcttccgtc
     2701 gcccctgccg ttacggtgat atcgctatcg gcatctggtt gatagccata caaacgttcc
     2761 gttttctgag caatcgcctc gcgcaaggcc tgcacgccgg tcatgggcgc gtattggttt
     2821 gccccctggg caacgtggta cgccagccgc tcctgtaaat agcgcggacc atcaaaatca
     2881 ggaaagcctt gcgacaggtt aatcgcctgg tgttgctgcg ccagcgcgct catctgggtg
     2941 aaaatagtgg tgccaagttg tggaagtttg ctttgtggaa tcagagggtt atttgtcatt
     3001 tttattgtac ctgacgaagt tgcgtgttga aggcactata acacgatgct agtatttggc
     3061 aatcaagacg tttagatgtc taaataaaac aataaggaca acacaacatg cctcacaatc
     3121 ctatccgcgt ggtcgtcggc ccggctaact acttttcaca tccaggaagt ttcaatcacc
     3181 tgcacgattt tttcactgat gaacaacttt ctcgcgcggt gtggatctac ggcgtacgcg
     3241 ccattgctgc ggcgcaaacc aaacttccgc cagcgtttgg acttccaggg gcaaagcata
     3301 ttttgtttcg tggtcattgc agcgaaagcg atgtacagca actggcggct gagtccggtg
     3361 acgatcgcag cgtggtgatt ggcgtcggcg gcggcgcact actcgacacc gcgaaagccc
     3421 tcgcccgccg tctcggtctg ccatttgttg ccgctccaac gatcgccgcc acctgcgccg
     3481 cctggacacc gctctccgtc tggtataacg atgccggaca ggcgctgcat tatgagattt
     3541 tcgacgacgc caattttatg gtgctggtgg aaccggagat tatcctcaat gcgccgcaac
     3601 aatatctgct ggcggggatc ggtgatacgc tggcgaaatg gtatgaagcg gtggtgctgg
     3661 ctccgcaacc agaaacgttg ccgctaaccg tgcgactggg gatcaataat gctcaggcca
     3721 ttcgcgacgt cttgttaaac agtagcgaac aggcgcttgc cgatcagcaa aatcagcagt
     3781 taacgcaatc attttgcgat gtggtggatg ccattattgc tggtggtggg atggtcggtg
     3841 gtctgggcga tcgttttacg cgtgtagcgg cggcccatgc cgtacataac ggtctgactg
     3901 tcctgccaca aaccgagaag tttctccacg gcaccaaagt cgcctacgga attctggtgc
     3961 aaagcgcctt gctgggtcag gatgatgtgc tggcgcaatt aaccggggcg tatcagcgtt
     4021 ttcatctgcc gactacgctg gcggagctgg aagtggatat caataatcag gcggagatcg
     4081 ataaagtgat tgcccacacc ttgcgcccgg tggagtccat tcattacctg ccagtcacgc
     4141 tgacaccaga tacgttgcgt gcagcgttcg aaaaagtgga atcgtttaaa gcctgacgtg
     4201 caaatttagc agcagcgcgc gccgcctttt ccaccgtagc gcgcgtcctg ccgctcacgg
     4261 aaaaactctt cgtaggtcat cggcgtttga tcgggatggt taacccgcat atgttcgaca
     4321 tagttgtcgt aatcaggcat accaatcatc agcttcgccg cctgacctaa atattttccg
     4381 gcttttgcca gtgaatcaaa catcttggcc tccgctaacc atgttgctga aagagtgccg
     4441 gatgcggcgt gaacgcctta tccggcctac aaatgctatc aacgcgctcg gtcagccccc
     4501 tcgccccatc ggggagaggg ttagggtgag ggggaaccca cggcgccatg ccaacattcg
     4561 ccaacatccc ccctcactct gactttagtg cgcgcctttt gcctgcacca cgatctcctc
     4621 gacattttcc ggcattggct catacggcgt ttctttcgcc gttggcttcg gctctttcaa
     4681 tgccgcaagt gccgtcttaa tcgagaacag tgccagaacc accacgacca ccataaagaa
     4741 gatggttaac ccggcatcca gacggttgtt gaacaccagt tgcgccagct gtgactcagt
     4801 atactgcgat ggaatattac cgctgtcgat cattgcctgg aacttattag caatggccag
     4861 gaagccgact ttcgcatccg ggctaaacgc tttctgccag cctgcggtca gggtacaaat
     4921 cagcagccag gccgttggta ccagcgccac ccaggcgtaa cgttgacgtt tcatcttgaa
     4981 caacaccacg gcacagagca tcagcgccat ccctgccagc atctggttgg caataccaaa
     5041 cagcggccac agagtgttaa taccgcccag cggatcgacc acaccctgat gcaggaagta
     5101 gccccacgcc agcacgcaca gcgccgttgc cagcaggtta gcaggcagtg aatcggtccg
     5161 tttcaggccc ggagaaacca cgcccagcag atcctgcaac ataaagcgcg cagcacgcgt
     5221 acctgcatcc accgccgtca gaataaacag agcttcaaac agaatggcga agtgatacca
     5281 gaacgccaca tccatcatgc cgcccagcgc gccatgcaga atgtaggcca tccccaccgc
     5341 caacgtcggc gcaccgcccg cacgggaaat gatcgactgt tctcccactt cgctggcaat
     5401 ctggttgagc gtatccggag taatcgcaaa gccccagcta ctcaccactt gcgcggcaga
     5461 agcgaccaca tccgccgtcc ctgccggagc cagcaccgcc atcgggctgt tcatggcgaa
     5521 gtacacgccc ggatcgatga tacaggcgga aaccagtgcc ataatcgcca cgaaggattc
     5581 cattaacatt ccaccgtagc cgataaaaca agcctgccct tcgttcgcca gcatcttcgg
     5641 cgtggtgccg gaagagatca gcgcatggaa gccagacacc gcgccacagg cgatggtgat
     5701 aaacaggaac gggaacaggt taccggtcca taccgggcca gtgccgtcaa caaatttggt
     5761 caacgcaggc atggtcagcg tcgggcgcat aatcaaaatg cctaccgcca gaccaacgat
     5821 cgtcccgatt ttcaggaagg tagagaggta gtcacgcgga gccagcagca accataccgg
     5881 cagcaccgcc gccacaaaac cgtaacccac cagcatccag gtcagctgca cgccggtaaa
     5941 gtcaaagtac ggtgcccagg tcggactttc tgccacccag ccgccagaaa taatggcgaa
     6001 aatcaggaat accagaccga tgaccgacac ttcaccaata cgccccggac gcagatagcg
     6061 caggtagatc cccatgaaca gcgccagcgg aatggtgaac gcaacggtat acgttcccca
     6121 cgggctatga gtcagggctt tcaccacgat catcgccagt actgcaagga taatgaccat
     6181 gatcataaag caggccacca gcgcgatcac cccggcggtt ggccccatct cttctttgac
     6241 cagctcaccc agcgaacgac cgtcacggcg cgtagaaaca aacagcacca tgaaatcctg
     6301 caccgcaccg gcgagaacca ccccggcgag tagccagatc atccctggca ggtaacccat
     6361 ttgcgccgcc agtaccggcc caaccaacgg acctgctccg gcaatggccg caaaatggtg
     6421 accgaacagc actttcttgt ccgtcggcac atagtccagc ccgtcgttat ggcgcaccgc
     6481 tggcgtcata cgcgtcgggt caaccgccag cacatttttg gcgatataca gaccataaaa
     6541 acggtaagcg atcagataga tacagaccga cgccaccaca atccacagcg cgttgatctg
     6601 ttccccacga tttaaagcaa tatatcccag agcaaatgct cccattacag agagcactgt
     6661 ccagacgagg tatttccctg atttgttcat agttgttatc cgtgtgcgtg tccatagaga
     6721 tgttacattt tgtttctata acatctttgt acgatttaac aacacagcaa ccatgtaaat
     6781 cggagttgaa accaatattt aacttaacaa tgttaactcg atcactccgt taaccgagtt
     6841 ttcctgcaat ctcatataat tctgtcgggt tcatcccaaa actgccgtac ccagccgaca
     6901 agtgcagcaa cgccgcccct gttcatcgaa aacgacgatt tcccagctct gattttgccg
     6961 cccaagatgc agcggctggc agacgccgcg taccttgcct tcagacaccg ggcgatgatg
     7021 tgtggcgtta agttccgtgc ccaccacaca ctgcccgtcg cgggtcatca taaatccggc
     7081 catcgatccc agcgtttcag ccagcgccgc cgacgcgccg ccatgcagca ggccaaacgg
     7141 ctgatgagta cggatatcaa ccggcatttc ggcttccagc acatcatcgc ccagacgggt
     7201 atacacaatt cccagatgcg ccaccattgt gttatcgctg gtggcgttca gttcgtcgag
     7261 cgttaaatgg cgtttccaga tcatgcttat gcccccagcg ttgagccgcc atcgaccaca
     7321 atatcctgta gggtgatatg gctggcgagg tcagaggcga ggaacaaaat cgtgttggcg
     7381 atctcttgtg gacgggcgat tttccccagc ggaatgccga gtttaaactg ctcgccaaag
     7441 ccgcgaatac gctgttcttc ggcgtcatcg ctcacccaca gcgtgcgttg catatcggta
     7501 tcggtggaac ccggcgaaac cacattacag cgcacgccgc tacccgccag ttccagcccg
     7561 acgctcaacg ccaggctttt cagcgccgct ttcgatgcgc cataagcact cataccaata
     7621 cgcggcgtgt gtgcggcgtc ggacgccaca gtgacaatcg ccccgccccg ctgacggcga
     7681 aactggttca tggtttgctg gaacaggtta aacgcaccgc cgacattaac cgcaaaagtc
     7741 tgctgccagt cctccttact gagctgatcg gtcgcgccca tgcgcaaaat tcccgccgca
     7801 ttgaccagca cgtccagtcg ctccgtttca gctaacagtc gctgacatac ctgcgccacc
     7861 tgcgcagcat cggcaacatc cattacttcg gtcgcaaagg gatattgctc ctgagcgaac
     7921 gcttgatcaa aacctgtaac tttcgctccc gcttcaacaa acgccagcgc cgtggcgtag
     7981 ccgatacctt tccccgcgcc ggttacccag acatttttac cgctgaaatc cattatttca
     8041 cctcgcggga gagtagcttc caccaggcgt cgatggtcgg gtttttcgcc aacatgacaa
     8101 agtcgatatc gccatgcact ttgcgccagc gcgccgccag cgccatcatg cgcaccgaat
     8161 ccagaccgta gtcgatcagg ttgtcgtcat cgaacggttc atcggactcg tccagcaacg
     8221 gcaggatcac ctcacgcagc gccgctttgc tggcggggat aggtgctggc agtaattctt
     8281 cggtcatcac cacccggcca gaacgtccgg ccacatattt cagcgacatc aaatgctcgt
     8341 cacggctgaa atcggccagc gcatccgcca ccataaacgg tttaatatcg cgcataaatg
     8401 cgtcggttgc ggtggtcata cagccaatgt gggcatatac cccggtaata atcagctggt
     8461 tacgtccact ctctttgagc atttgctcca gcggagaacg atgaaacgcg ctgtagcgcc
     8521 acttcaccag caccgtgtcg tcggcatctg gcgtcagtcg atccaccacc ttttgctgtt
     8581 ccggcgaacg ggtcaggccc ggcccccaca tatcattcaa cagcgcccga tcttcatcgc
     8641 tctgctcttt cggctgggcg gtgtaataaa ccgggatatt gtgctgtttg cagtagtcgc
     8701 gcagcgcagc aatattcgcg atcacctgct ccatcatcgg gcagttctcg ccccagaagc
     8761 tgacaaaata gtcctgcata tcatggatta acaacgcggc acgttgcggt tcaaaggccc
     8821 agtcaacttt attctgcgga atatcgtgag actccggcag tgcgtaagcc tgtaattttg
     8881 gaatagccat cgtgttctct ccttcaggct gatgcgcgtg acgccagcca ctgacgtaat
     8941 tgttttttat cgactttccc gaccgccgtc agcggaagtg aatccacaca ctccacgcga
     9001 tccggtaatt taaattcggc aataccctgt tcacgcagga aacgacgcac ctgcaccgcg
     9061 cgaagcggct cttttaccac cagataagca cagctttttt cgcccatcag ctcatcttcc
     9121 atactgacca gtgcggcgta gatcaccgcc ggatggcgca gtaacaggtt ttcgatctct
     9181 tcggcagcga tcttctcgcc gccacggttg atctgatctt tctcgcgccc ctgcacggtg
     9241 atgtaaccct ctggatcaat agagatcaga tcgccggaac agtaaaaacc gttggcatca
     9301 aaggcgctgg cattgtgctg cgggcttttg taatagccac ggaaggtata cggcccgcgc
     9361 gtcatcagtc gtccgacttc cccttgcggc agtggatttc cttcggcatc ggcaacccat
     9421 acttcgtcat ccggacacat tgggtaaccc tgggtatgga taattttctc cgcgctatca
     9481 tcaagacggg tatagttcac cagcccttcc gccataccaa acacctgctg caactggcaa
     9541 ccaatctcag cgggaatacg cgccgcaagc gtggcagaaa gacgtgcgcc accgacctgt
     9601 aacagtttca gcgaggcaag ctgcgcccgg ctttcgcctt cggtcagcgc ctgcaaccac
     9661 aggctgactg ccggcggcac cagcgcggta acgttaatct gatgtttttc aatcaatggg
     9721 aagcaaagcg tggcgctggg atcggccgcc agaacaaccg ttcctccggc aagaaagacg
     9781 cccagcgatc ccggcgaact catggcgtag ttatgagccg ccgggatcgc gcacaggtag
     9841 cgtgtctgtt gtgtgaactg acaaatctcg acgctacgac gcacgctgta gtagtagtcg
     9901 ttatgagtgc gcgggatcag cttcggtgta ccggtggtgc cgccggaaag ctggaaatag
     9961 accacttcat cagcaggtga cggcgtagca gtaaaaccgt ctgccggatg gttaatcgca
    10021 tcctgcaagt tatgctcacc gctgtcgttg agtagctgca ccacgcgaat ggaagaatgc
    10081 tctgcaacaa atgtgttgag gaaatcatcc ccgctaaaca gcgcatgttg gcgatcggca
    10141 atcagcaatg cgggttcaat ctggctggca taggcgttca gttcactacg ctgatggctg
    10201 aacaacgcca gcaccggcgc aacgcccagt ttcagcagcg cgaaaaaggt aatgtaaagt
    10261 tcagcgacgt tacccagttg taccagcgcg gtttcaccag gtttaatgcc ctgacggcgt
    10321 aaactacacg cgaggttatc cgccgcctga ttcagctccc ggtaactcaa ctgtcgctcg
    10381 ccgtcgataa ccgcgatgct gtcactcgca gcgtggcgag tcagaatgtc agtcagtggc
    10441 aaatcctgcc agtagccttt ttcccgatag cgacgggcaa actcttccgg ccagcgggtg
    10501 aatggaatgc tcatcctcgc tccttaatgc aatccaaaaa cgttcaacat ggtagaaagt
    10561 ttgacgcctg tttcgcgcca ctcacccaac ggtgacgacg caggcacaat ccccgctccg
    10621 gcaaacagac gcacctgatt ttcccgcagc ttcgcgcagc ggatggtcac cacccattcg
    10681 ccgttacctt cgctgtcaca ccaacccaca atgccgccaa acagttcgcg gtcgaacggc
    10741 tccagttcag caataacctg ggtcgcggcc tgatgcggga aaccgctcag cgcgggggtc
    10801 ggatgcagca gacaggccag agtcagtgcg ttttcttgcg aattcgcttt accttcaaag
    10861 ggagttgcga gatgccacag cgtcggcgtg gtaatcaatt gtggagagga aggaacgtgt
    10921 aactcactac tgcgttcgcg cagtacctct ttcatcgcct gagtcaccag ttcatgttca
    10981 tggcgatctt tttctgacgc cagcagacga ttacccgctt cgcgatcgag cacttcatcc
    11041 ggctgacgac gtgcggaacc ggctaacgga atggagctaa aacgctcacc gtctttacgt
    11101 agcagcagtt ccgggctggc ccccagcaga acgccaccat ctgccagcgg gacatggaag
    11161 ttgtaactaa ccgggttttg cgcaatcaac cgttccagca atacgccact atcaatggcg
    11221 gcgtcagtgg tgatatcaat caaccgtgac aacaccactt tgtcgacctg cggcgtggcg
    11281 gtaagtgcgg cggcgcgggc aaccatctgt tcaaacgtgg tttgctccgg aattgcctgg
    11341 cgttccacca cgttcagcga ctggctgcgg gtgaaacggc gtgcggaagc ttgtttttcc
    11401 tgacgggaga acgactgcca ggattcagga atatacagcg acgaaggctg acgtggatcg
    11461 aaaggaatcg ccccgaccat caccggattt ttgatgccct gcgctttggc atcggcaaac
    11521 agcgcggcga gtttttgctg gaagggactg tcgggcgaat ccccgttcac agccggttca
    11581 tcgaagcggg cgaaacatcc tgacgtcgta aaactgcggt acggcgacat aaagaaaaag
    11641 cgattgggcg caagtgttgc catggtctgc tgtacttcct cagccagtga cgtatccata
    11701 tcatcctcca caaaatgata aaggctttaa taatgattat catttatatt ttcggtcgct
    11761 aacctaaaag caaacgcgca ctatgtcaac tcttgaggta acgcaatctg caactcgggg
    11821 ttgcatctgg ttgtggttat aatgaaaatg agaagcatta acttcattaa taacaggagg
    11881 ctgatttgtg agactcgccc cgctctaccg caatgccctt ctattaacag gacttttgtt
    11941 ttcaggaata gccgcagttc aggccgccga ctggccgcgt cagattactg acagccgtgg
    12001 cactcatacc ctggaaagcc agccgcagcg tattgtttcc accagcgtca ccctgaccgg
    12061 ctcactgctg gcgattgatg ctccggtgat cgccagcggc gcgaccacgc cgaataaccg
    12121 cgtcgcggat gaccagggct ttttacgcca gtggagcaag gtggcgaaag aacgcaaact
    12181 gcaacggctc tatatcggcg aaccgagcac cgaagccgtt gccgcgcaaa tgccggatct
    12241 gattttaatt agcgcaaccg gcggggattc ggcgctggcg ctgtatgatc agctttccac
    12301 catcgccccg acattaatca tcaattacga cgacaaaagc tggcaggcgc tgttaacgca
    12361 acttggcgaa attaccgggc atgagaaaca agcggcagag cggattgcgc agtttgataa
    12421 gcaactggcg gcggcgaaag agcaaatcaa attaccgccg cagccggtca ctgccattgt
    12481 ctatactgcc gctgcacaca gtgccaatct ctggacgccg gaatcagcac aagggcagat
    12541 gctggaacaa ctcggcttta cgctggcgaa gttacccgca gggttaaatg ccagccaaag
    12601 ccagggtaaa cgccatgaca tcattcagct tggtggggaa aatctggctg cagggttaaa
    12661 tggcgagtca ctattcctgt tcgccggtga tcagaaagat gccgatgcta tttatgctaa
    12721 tccgctgctc gcgcacctgc ctgcagtaca aaacaagcag gtttatgcgc tgggaaccga
    12781 gacgttccgt ctggattact acagcgccat gcaagtgctg gaacgactta aggcgctgtt
    12841 ttaagcatta accgtcagac gctgtcacct gcggcggcgt ctggcgaaaa cgtcgcaact
    12901 ccaccagcac cagcaataac aacacgccga taatcaataa accaaaaccg ctcgcgcttg
    12961 cggaagcaac cggtgtcatc atcgctccca aaccacccag cagcgccgcg cctatggcat
    13021 cgcccgtcac gttttgtgcc gtccacaaac cgttaatccg ccctaacatc gcttccgggg
    13081 tttgcgtttg cagcattgtg tattgcagca acgagctgac cgcactcaac cagccgaaca
    13141 gcgccagaca aaccacgcct aaaatccaca tcggcatcag gccaaacaga ccaatggcga
    13201 ggaacgatcc cagcgtggag agcagcatca ataaccctgg tcgcgcgcta tgcgccagct
    13261 tcccgctggt taacgcgcca atagccgcgc cgagcgggat cgccgcatag agaaaaccaa
    13321 tctgtgccgc cgacatctgc cagttgtcag ccagcgccgg atacagtacc cgcaccgcac
    13381 tcgccatcgt caataaacca cccagcagcg caatccctcc caccagcggg ctggcgagta
    13441 gaaaacgaaa tcctgccagt aatgatttca acggatgctc acgcggctgc ggtggcggtg
    13501 gcagtgccgg aaggcttaac aacggtagca aggtaataaa cgtgcccgcc gccgccagcc
    13561 cgtagttcca ggccacaccg ccagtcgcca gcagtaaacc gccaatcatg ggcgaaatca
    13621 ccgaccccag acgcacggtc aacatggtga tcgccccggc ctgcattaag ttttcacgcc
    13681 ctaccagtgc tggtgtcgcc gccaatagcg ccgtaacgcc aagcgatgcg aaaaaaccat
    13741 cccataaacc aagtaaatag attgccagca atgacggctc cggcagcagc gcgttcaggc
    13801 acagtccaat gaagccaatg ccacaggtgc cgcgcgccag caaaatcact tttttgcgct
    13861 catagcgatc cgccagcaca ccgccgacca tcaggccaac aaacatcgcg ccgccggtca
    13921 gcgtcaccga aagccccacc tgccaggtgg aatgtgtcat catctggatc tgcaccggca
    13981 ccgcgacgcc gagcaaaccc agagacacaa ttgagatgaa acgagcgagg aatactgcgc
    14041 gaaacgccgg gtgcgttttc aacaggctga ggttaagcag ccaggattgt ttattcatta
    14101 caatgccttg ccatcaaata ttttaaatac ctattcttag gctgcacatg ctaacatatc
    14161 caaataagat cgataacgat aattaatttc attatcatgg aagttcgtat gtctggttct
    14221 gttgccgtga cacgcgccat tgccgtgccc ggattgctgt tattactgat tatcgcgacg
    14281 gcattaagcc tgctcattgg ggcaaaatca ctccccgctt ccgtagtgct ggaggccttc
    14341 tccggcacct gccagagcgc cgactgcacc atcgtgctcg acgcgcggct gccgcgtacc
    14401 cttgccggtt tactggcagg cggcgcgctt ggccttgccg gggcgttaat gcaaaccctc
    14461 acccgaaacc cacttgccga ccccggcttg cttggcgtga acgccggagc cagctttgcc
    14521 attgtgctgg gtgcggcgct gtttggttac tcttccgcac aggaacaact ggcgatggcc
    14581 ttcgccgggg cgctggtggc ctcgttgatt gttgccttta ccggcagcca gggcggtggg
    14641 cagttaagtc cggtgcgttt aaccctggcg ggcgtggcgc tggcggcggt gctggaaggg
    14701 ctgaccagcg gcatcgccct gcttaatcct gacgtctacg atcaactgcg tttctggcaa
    14761 gccggttcgc tggatattcg caatctacat accttaaaag tggtgctgat cccagtgctg
    14821 atcgccggag caactgcgct attactgagt cgcgcgctga acagtttaag ccttggcagc
    14881 gacaccgcga cggcgctggg cagtcgcgtg gcgcgcacac agttgattgg tctgctggcg
    14941 attaccgtgc tttgtggtag tgcgacggcg gtagttggcc ctattgcctt tattggcctg
    15001 atgatgccgc atatggcgcg ttggctggtg ggtgccgatc atcgctggtc gctgcccgtc
    15061 acgctacttg ctacccctgc cctgctgctg tttgccgata tcatcgggcg ggtgattgtg
    15121 cccggtgaac tgcgcgtttc tgtggtcagt gcgtttattg gtgcaccggt gctgatcttc
    15181 ctcgtgcgac gtaaaacgcg aggtggtgca tgatttacgt ctctcgccga ttactcatca
    15241 cctgtttgct gctgatttcc gcctgtgtgg ttgcaggtat ctggggatta cgcagcggtg
    15301 ccgtcacgct ggaaatctcg caggtattcg ccgcgctgat gggcgatgcg ccgcgcagta
    15361 tgacgatggt ggtcaccgaa tggcgtttac cacgcgtgct gatggcgctg ttgattggcg
    15421 cggcactggg cgtcagcggt gcgatttttc agtcgctgat gcgtaaccca ctcggcagcc
    15481 ctgacgtaat gggctttaac accggggcgt ggagcggcgt gttggtggcg atggtgctgt
    15541 ttggtcagga tctgacggct atcgcgctgg cagcaatggt gggcggcatt atcacttcgc
    15601 tgttggtctg gctgctcgcc tggcgtaacg gtatcgacac ctttcggttg attattatcg
    15661 gtatcggcgt tcgcgccatg ctggtggcct ttaatacctg gctgttgctg aaagcgtctt
    15721 tagaaacggc gctaacagca ggtttgtgga atgccggatc gctcaacggc ctgacgtggg
    15781 caaaaacctc gccttccgca cccatcatta tattgatgct aattgccgcc gccttactgg
    15841 tacgccggat gcgcttgctg gaaatgggcg atgataccgc gtgtgcgctg ggcgtcagcg
    15901 tcgaacgttc gcgtctgtta atgatgctgg ttgcagtggt gcttaccgct gcggcaacag
    15961 cgcttgccgg gccgatttcc tttattgctt tagtcgcgcc gcacattgcc cgacgcatta
    16021 gcggcaccgc tcgctggggg ctaacccagg cggcgctatg cggggcgctg ttactgctgg
    16081 cggccgatct ctgcgcccaa caactgttta tgccgtatca acttccggtt ggcgtcgtta
    16141 ccgtcagcct cggcggtatt taccttatcg ttttgttaat tcaggagtct cgcaaaaaat
    16201 gaccgaatca gtagcccgtt tgcgcggcga acagttaacc ctgggatatg gcaaatatac
    16261 cgttgcggaa aatctgactg tagaaatacc tgatggtcac ttcacggcaa ttatcgggcc
    16321 aaatggctgc ggtaaatcca cgttgctgcg taccttaagc cgcctgatga cgcctgctca
    16381 tgggcatgtc tggctggatg gcgagcacat tcaacattac gccagtaaag aagttgcccg
    16441 gcggattggt ctgttggcgc aaaatgccac cacgctggga gatatcaccg tgcaggagct
    16501 ggtggcgcgt gggcgttatc cacatcaacc gctgtttacc cgttggcgca aagaagatga
    16561 agaggcggta acgaaagcga tgcaggccac gggaataact catcttgcag atcaaagcgt
    16621 ggataccctt tccggtgggc aacgccagcg agcgtggatc gcgatggtgc tggcccagga
    16681 aacggcaatt atgctgctcg acgaacccac gacctggctg gatatcagtc atcagattga
    16741 tttgctggaa ctgttaagcg aactgaaccg cgagaaaggc tataccctgg cggcggtgct
    16801 gcacgatctt aatcaggcct gtcgttacgc cagccatttg attgcattgc gagaagggaa
    16861 aattgttgct cagggagcgc cgaaggagat tgtcactgct gaactgattg agcgcattta
    16921 tggtctccgc tgcatgatca ttgacgatcc ggtagccgga acgccgctcg tggtgccgct
    16981 cggacgaacg gcaccttcaa ccgcaaatag ttaaactaag tggtctgcca tcatggcatc
    17041 ctgttttctg gatgccatcg catagcgcaa taacacgcta ccacaagcca ccatcccgcc
    17101 gatcagcgcg gaaaggatca caataatcgc cttacccgga ccgtcttttt tcactggcaa
    17161 tgacgggctt aactgatatt taaacggcgt aaaattcaca tcgttgatat ttgcttttgt
    17221 taactgctca acaagatact gacgattacg taattcaccg ttcagttccg caacgtcagt
    17281 gaccgctttt tcaatttcca gtttgcgttc aataccgtct gcgccgagag aaatagagaa
    17341 atcggggtca tctttaacgg cctgaccatt actgtaaacg ggctttttaa ttcctgccgc
    17401 gttggcaatg tcgagtgaat aattgaggcg ctgaatgttt gcatcaagtt gatttttcat
    17461 tttaatgcgg tcctgagcca gtttttcttt ttcaaactgg gttttgatct ccagtttatt
    17521 acggacgttt tctatcgact ctttcaccac caacgcagag atataatcga tatacccgct
    17581 caaaacggtc tgcgcctctt cactggttgg cgcggtaaaa cttagcgtcc aggaggtata
    17641 cagtgacggt tcatcttttt tcttactggc attgtcatca accgctttca ttttttcgct
    17701 caatgcgaca attgcgcgat gcaaatccag ttcgtcgatt ttcgcctctt ttaattggtc
    17761 catcacataa ggtgatgaac gcaggtactc ttccagcaag ctaaccgact gaaacttctt
    17821 gataaacagg ttaaatgctt ctgtacgatc aattttgata tccagatcca gcacacgaag
    17881 cttagtgaat gttttctcca actcttgcca ctgaacaggt tctggaggcg tgacaaccgc
    17941 cgcgctggtc catttttgcg gcaggatgaa agagatcagc aagcctgcac aggcaaacgc
    18001 aaaaacgacc gccatgaccg tttttttggc ccgccataaa actgagatta gattaagtaa
    18061 atcaatttca ttattactgg gcgac
//
LOCUS       DBFIFI010000082        17432 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.082, whole genome shotgun sequence.
ACCESSION   DBFIFI010000082 DBFIFI010000000
VERSION     DBFIFI010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17432
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.082"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..2052
                     /locus_tag="N7B39_002636"
     CDS             <1..2052
                     /locus_tag="N7B39_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HGU2422591.1"
                     /translation="LVLDDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGQVIFADFN
                     PSKRPWSVASDDSNNSWNPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWR
                     YLNEYLDQISANDEVLFLKQGHEIIAKNQLAREKLIIYNSEGDYNIIDSVDTEYIEKT
                     SAVPNNALFEIYFYYPGGNLLNASDKLFYLPFAFIIIVLLVVYLMTTRVFRRQFSEMT
                     ELINTLAFLPDSTDQIEALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQ
                     ESGFIKNMAIIESNNNQYLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQ
                     CCDIVTFNADLYLLLCRENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENA
                     WSYAEKLKLAISSIRDHMFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQP
                     IVDINTRAILGAEALCRWVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRH
                     FSQRASLKDDFLLHINVSPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERIN
                     EHFYLNIEQLRKQGLRISIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIV
                     RIIFDLARYNRIPVIAEGVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVK
                     E"
     gene            2060..3388
                     /locus_tag="N7B39_002637"
     CDS             2060..3388
                     /locus_tag="N7B39_002637"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HGU2422592.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(3458..3787)
                     /locus_tag="N7B39_002638"
     CDS             complement(3458..3787)
                     /locus_tag="N7B39_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU2422593.1"
                     /translation="MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAK
                     VKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSKWERNEIKPSGPALRILNTLAIK
                     GPEVFAK"
     gene            complement(3777..4163)
                     /locus_tag="N7B39_002639"
     CDS             complement(3777..4163)
                     /locus_tag="N7B39_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGU2422594.1"
                     /translation="MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVI
                     KKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSGLSSKGSKEIEDDELAAYKKMAN
                     AFLAFSNKQIEDLIETGFLIEVKNER"
     gene            complement(4389..5714)
                     /gene="rimO"
                     /locus_tag="N7B39_002640"
     CDS             complement(4389..5714)
                     /gene="rimO"
                     /locus_tag="N7B39_002640"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HGU2422595.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            5927..6310
                     /gene="bssR"
                     /locus_tag="N7B39_002641"
     CDS             5927..6310
                     /gene="bssR"
                     /locus_tag="N7B39_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HGU2422596.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            6421..7536
                     /gene="yliI"
                     /locus_tag="N7B39_002642"
     CDS             6421..7536
                     /gene="yliI"
                     /locus_tag="N7B39_002642"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HGU2422597.1"
                     /translation="MHRQFFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(7533..8159)
                     /gene="gstB"
                     /locus_tag="N7B39_002643"
     CDS             complement(7533..8159)
                     /gene="gstB"
                     /locus_tag="N7B39_002643"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HGU2422598.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            8405..9607
                     /gene="dacC"
                     /locus_tag="N7B39_002644"
     CDS             8405..9607
                     /gene="dacC"
                     /locus_tag="N7B39_002644"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HGU2422599.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            complement(9654..10412)
                     /gene="deoR"
                     /locus_tag="N7B39_002645"
     CDS             complement(9654..10412)
                     /gene="deoR"
                     /locus_tag="N7B39_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HGU2422600.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTQRIKLMY"
     gene            complement(10470..11066)
                     /gene="ybjG"
                     /locus_tag="N7B39_002646"
     CDS             complement(10470..11066)
                     /gene="ybjG"
                     /locus_tag="N7B39_002646"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HGU2422601.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLLVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            11351..12583
                     /gene="mdfA"
                     /locus_tag="N7B39_002647"
     CDS             11351..12583
                     /gene="mdfA"
                     /locus_tag="N7B39_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HGU2422602.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            complement(12624..12908)
                     /gene="ybjH"
                     /locus_tag="N7B39_002648"
     CDS             complement(12624..12908)
                     /gene="ybjH"
                     /locus_tag="N7B39_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HGU2422603.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYHPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(12994..13809)
                     /gene="ybjI"
                     /locus_tag="N7B39_002649"
     CDS             complement(12994..13809)
                     /gene="ybjI"
                     /locus_tag="N7B39_002649"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HGU2422604.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKMVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKTLHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGNAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(13809..15017)
                     /locus_tag="N7B39_002650"
     CDS             complement(13809..15017)
                     /locus_tag="N7B39_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2422605.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALVGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            15101..15637
                     /gene="rcdA"
                     /locus_tag="N7B39_002651"
     CDS             15101..15637
                     /gene="rcdA"
                     /locus_tag="N7B39_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HGU2422606.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(15812..>17432)
                     /locus_tag="N7B39_002652"
     CDS             complement(15812..>17432)
                     /locus_tag="N7B39_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HGU2422607.1"
                     /translation="LGLCLGKLRLGSIQLGNSIGVLVVSLLLGQQHFSINTDALNLGF
                     MLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGSALVIALGLGKLFGWDIGLTAGM
                     LAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLSLGYALTYLIGLVSLIVGARYLP
                     KLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAYRVGPELVAWTDGKNLRELGIYR
                     QTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYPDAHARLDPSFRNGKEVFDRDLL
                     DMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRVIRSQIEMPIDDNVVLNKGDVLQ
                     VSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVIGLMIGMITFQFSTFSFGMGNAA
                     GLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLMVFMAGVGLSAGSGINNGLGAIG
                     GQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGAMMGARTCAPAMEIISDTARSNI
                     PALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
BASE COUNT         4515 a         4165 c         4322 g         4430 t
ORIGIN      
        1 ctggtacttg atgataataa atattatctc ttttcgcgga gggcgaacga taaaatcgtt
       61 gtttatcatc aggaacaagt aaatggaccg cttgtcgacg agtcagggca ggttattttt
      121 gccgatttta acccatcgaa acgaccgtgg tcggtggctt cagatgactc taacaacagc
      181 tggaatccgg cctacaattg ctttgatcgt ccgggtaaaa aatgtatctc ttttacgcta
      241 cgcatcaacg gcaaagatca cgatttgtta gcggtggata aaattcatgt cgatttaaac
      301 tggcgatatc tgaacgagta tcttgatcaa atcagcgcta atgatgaagt tctatttttg
      361 aaacaaggcc atgagatcat tgccaagaat caactcgctc gtgaaaaact gattatttat
      421 aatagcgaag gtgattataa tattattgat tctgtcgata ctgaatatat cgaaaaaaca
      481 tcagcggtgc caaataacgc attattcgaa atctattttt attatccggg cggtaattta
      541 ttgaacgcat cagataaact tttttatctg ccgtttgcgt tcattattat cgtattgctg
      601 gtggtttatt taatgaccac tcgtgtgttc cgtcggcaat tttctgaaat gacagagctg
      661 attaatacgc tggcgttttt gcctgactca acggatcaga tcgaggctct gaaaattcgc
      721 gaaggcgatg cgaaagagat tatcagcatc aaaaattcga tcgcggaaat gaaagatgcc
      781 gaaattgaac ggtcaaataa attgctctca ctgatctctt acgatcagga aagtggtttt
      841 attaaaaata tggcgattat tgagtccaac aataatcagt atctggctgt ggggatcatc
      901 aaactgtgtg gtctggaagc cgtggaagct gtgtttggtg ttgatgaacg caataaaatc
      961 gtcaggaaat tgtgtcagcg aattgctgag aaatatgcac aatgctgcga tatcgtgaca
     1021 ttcaatgccg atctctattt acttctgtgt cgggaaaatg tacagacatt tacccgtaaa
     1081 atagcgatgg taaacgattt tgacagcagc tttggctacc gcaatctgcg catccataag
     1141 tctgccattt gtgaaccttt gcagggggaa aacgcctgga gttacgcaga aaaactgaaa
     1201 ctggcgattt ccagtatccg tgaccatatg ttctccgagt ttattttctg tgatgacgcg
     1261 aaactcaacg aaatagaaga gaatatctgg attgcgcgta atattcgcca tgcaatggaa
     1321 attggcgaac tattcctcgt ctatcaaccg atcgttgata ttaacacccg cgccattctg
     1381 ggcgcggagg cgttgtgccg ttgggtgtct gcggagcggg ggatcatttc accgctgaag
     1441 ttcattacca ttgctgaaga tatcgggttt atcaatgagc tgggttatca gattattaaa
     1501 acggcaatgg gtgaattcag acattttagt cagcgtgcat cgctgaagga tgatttctta
     1561 ctgcatatta atgtttcgcc ctggcagtta aacgaaccgc actttcatga gcgttttacc
     1621 accatcatga aagaaaatgg cctgaaggcg aacagcctct gtgttgagat cactgaaacc
     1681 gtgatcgagc gaattaatga acatttttat ctcaatattg aacaactgcg taaacaaggg
     1741 ttacggatat cgattgatga ctttggcacc ggtttgtcaa acctgaaacg tttttatgaa
     1801 attaatccag acagcataaa ggtggactcg caatttaccg gcgatatttt cggtactgcg
     1861 ggaaaaattg tgcgcattat tttcgacctg gcacgctata accggatccc ggtgattgcg
     1921 gaaggcgtag agagcgaaga cgttgcgcgc gaattaatca aattaggatg tgttcaggct
     1981 caggggtatc tgtatcagaa acccatgcca ttctccgcct gggataaaag tggaaaatta
     2041 gtaaaagagt agtttacgta tgtccagaat caataagttc gtacttacag tcagtctgct
     2101 gatttttatc atgatttcag cagttgcctg cgggatctac actcaaatgg taaaggaacg
     2161 ggtgtatagc ctgaaacagt ccgttattga tactgctttt gcggtggcaa atattgctga
     2221 atatcggcgt agcgtggcaa ttgatcttat caacacgcta aatcccacgg aggaacagct
     2281 gttggttggc ttacgcacag cttacgccga ctcggtttca ccctcttatt tgtacgatgt
     2341 cggtccttat ctgatttcca gtgacgaatg tattcaggta aaggagttcg agaaaaatta
     2401 ttgtgcagat attatgcagg ttgtgaagta tcgacatgtc aaaaatacag ggtttatctc
     2461 ttttgacggt aaaaccttcg tctattacct ctatccggta actcacaatc gtagtctgat
     2521 atttttgctt ggtctggagc gtttttcttt actgtcaaaa tcgctggcga tggacagcga
     2581 gaacctgatg ttctctctgt ttaagaacgg taaaccggtg accggtgatg aatataatgc
     2641 taaaaacgcc atcttcaccg tttcggaagc gatggagcac ttcgcctatt tgccgaccgg
     2701 attgtacgta tttgcgtata aaaaagatgt ttatttgcgg gtctgtacat tgattatttt
     2761 ctttgccgca ttggtggcag tgatatcggg tgccagttgc ctctatctgg tacgcagagt
     2821 gattaatcgt ggtattgtgg agaaagaagc cattattaat aaccattttg aacgcgtact
     2881 ggatggcggg cttttctttt cggctgccga tgtcaaaaaa ctctacagta tgtataactc
     2941 ggcgttcctg gacgacctga ccaaagcaat gggtagaaaa tcctttgacg aagatttaaa
     3001 agcgctgccg gaaaaaggcg gttatttgtg cctgtttgac gtcgataaat tcaaaaatat
     3061 taacgacacc ttcggtcatt tgctgggcga tgaagtgttg atgaaagtgg tgaaaatcct
     3121 taaatcacag atcccggtag ataaaggtaa agtctaccgc ttcggcggtg acgaatttgc
     3181 ggtgatttat actggcggca cgctggaaga gttactatcg attctaaaag aaatcgttca
     3241 tttccaggtg ggaagcatta atttaagcac cagtatcggt gtagcgcatt caaatgaatg
     3301 tactaccgtc gaacgcttga aaatgctggc ggatgagcgg ctgtataaga gtaagaaaaa
     3361 cggcagggct cagattagct ggcagtagaa ttagaatagt gttagtaccc ctgaatcaag
     3421 gggtactaat gaatgtggta aatcatttgc aaacatttta ctttgcaaaa acttccggcc
     3481 cttttattgc caatgtattc aaaatacgta gtgccgggcc gctcggtttt atttcattac
     3541 gttcccattt cgagacactt tcttttgaca ttcccattgt atgtgccagc acggattgtg
     3601 acatgccgta tcgggtgcgg atggctttga tctcttcacc cgtcattgat ttgactttgg
     3661 ctatacgggc gctgacttca cgggctttga ccatgacggc aatggcatca acctcactat
     3721 cagacatcag gcccagtgca ttcagttcct ttgcatcttc cagcatcatt tgtaatttat
     3781 cgctcatttt tcacctcaat caagaagcca gtttcgatca aatcctctat ttgcttattg
     3841 ctaaaggcca gaaatgcatt tgccattttt ttgtaggctg ccagttcatc atcttcgatt
     3901 tctttgcttc ctttactgct tagaccactc ttggaccaac catcataaaa aaacacatga
     3961 tttgctgatt taaaaaagat aattgttctg gctccaccac tctttccttg ttggaggggt
     4021 aaccgttttt tgatgacacc gctacctaaa tcagcctccc atatgccttg catcacttct
     4081 tcagcggcag ccatgagaac tttatcgtga ataccaagtt ttttcgtgct ttggctgaat
     4141 cgccttgcct tatacacacc catacatcca tccttgtggt aatgtttact atagtaacca
     4201 ttacgcttat ttcaagtaat tgcgatcaaa actttaattt ggtgtgaaaa agtgagagtt
     4261 gcctcgagta cgattcattt ttctcgattt atgcgtcagg ggattttgtg cgtaagggta
     4321 ggacatcggg aagggtagta gaggcagatc gaagaacaat atccttgtta gatctgcctt
     4381 tagattaatt aaacccggct accccacaaa tcgtactcat cggcgtgctc gactttcaca
     4441 cgcaggatat cacccggctt aacgttggtt tcaccattga gataaaccgc accgtcgatt
     4501 tccggtgcat ctgccatgct gcgaccaatc gcgccttctt cgtccacttc gtcgataatc
     4561 accagaattt cacggcccac tttctcttgc aggcgctcgg cggaaatctg ctgctgcaac
     4621 tgcatgaaac ggttccagcg ttcttctttc acttcttccg gaacctggtc aggcagggca
     4681 ttggcgtctg caccttcaac cgggctgtat ttaaagcagc caacgcgatc cagacgcgct
     4741 tctttcagga agtcgagtag catctggaaa tcttcttccg tctcgccagg gaagccgaca
     4801 ataaaggttg agcgtagggt cagttccggg cagatttcgc gccactgttt gatgcgcgcc
     4861 agttggcgat ctacagaacc cggacgcttc atcagtttga gaatgcgcgg gctggcgtgc
     4921 tgcaacggaa tgtccagata cggcaggatt ttgccttctg ccatcagtgg gatgacgtcg
     4981 tccacatgcg gataagggta aacgtagtgc agacgtgtcc agatccccag tttcgataac
     5041 tgttcgcaca ggctgaccat gctggttttt accggctcgc cgttgtggaa gccagtacga
     5101 tgtttaacat caacgccata ggcggaagta tcctgcgaga tcaccagaat ctctttaacg
     5161 cccgcatcta ccagacgttt cgcttcactt aatacttcgc caatcggacg gctcaccagg
     5221 tcgccgcgca tagacggaat aatgcagaag gtgcagcggt gattacagcc ttcagaaatt
     5281 ttcagatagg cataatgacg cggcgtcagt ttgacacctt gttctggcac caggctcagg
     5341 aatgggttgt gtttcggttt tggcacgtag tgatgaacgt gctccagaac ctgctcatag
     5401 ctatgaggcc cggtgatttc cagcactttc gggtggactt cgcggatctg atcttctttt
     5461 gcccccagac aaccggtcac aattaccttg ccgttttcat tcaacgcttc accaatggct
     5521 tccagtgatt cttgtaccgc gctgtcaata aagccgcagg tgttgacgat caccatgtcc
     5581 gcatcgtcat agctcggtac cacgtcataa ccttcagtgc ggagttcggt gagaatacgc
     5641 tctgaatcga caaggttttt cggacagcca agggaaacaa agccgatttt cggctgggga
     5701 gttactttgc tcatagctta aaaaatattc agttacagga aaggtcaggg cagggattct
     5761 acagagttct ggataaaatt tgtatcgcaa tctcattcgc tggcggaggc gaaggaaatg
     5821 taaattttgt taattcggcg tgaagaattg atcctggaca gcattatgct caaaaaatag
     5881 ccatactatt taattgcaac aaggctggaa agaggaggat cgaagtatgt tcgttgacag
     5941 acagcgaatc gatctgctga accggttgat cgacgcacgc gttgacctcg ccgcatacgt
     6001 gcaactgagg aaggcaaaag gatacatgtc cgtcagcgaa agcaatcatc tacgagataa
     6061 cttttttaaa ctgaatcgcg aactgcacga taaatcgctg cggttgaatc ttcatctgga
     6121 tcaggaagag tggagtgctc ttcatcatgc tgaagaagca ttagcgacag ccgcagtatg
     6181 tttgatgagt gggcaccatg attgcccgac tgttattacc gtcaacgccg ataagcttga
     6241 aaattgtctg atgagcttaa cgctgagtat ccagagcctg cagaagcacg ccatgcttga
     6301 gaaggcctga aaactaaggg ggagaaagcg tctccccctt catgtttaag tttttgtaaa
     6361 aatgaatttg ttatctcctc cactgactac gctttaagcc agagtcaatc cggaggcgtt
     6421 atgcatcgac aattcttttt ccttgtgccc cttatttgtc tttcttccgc tctctgggcg
     6481 gctcctgcaa cggtaaatgt cgaagtactg caagacaaac tcgaccatcc ctgggcactg
     6541 gcctttttac ccgataacca cggtatgtta atcactctgc gcggcggcga gttgcgtcac
     6601 tggcaagcag gaaaaggatt atctgcgccg ctttccggag ttccggacgt ttgggcgcac
     6661 gggcagggcg gcctgctgga cgtggtttta gcgcctgatt ttgctcagtc tcgccgcatc
     6721 tggttaagtt attccgaagt tggcgatgat ggcaaagccg gaactgctgt ggggtatggc
     6781 cgcttaagtg atgatctctc aaaagtgacc gactttcgca ccgtctttcg ccagatgcca
     6841 aaactgtcta ccggcaacca ttttggcggg cggctggtat tcgacggtaa aggttatctt
     6901 tttattgctc tgggcgaaaa caatcagcgc ccgacggcgc aggatctgga taaattacag
     6961 ggcaaactgg tgcgtctgac cgaccagggc gaaatcccgg atgataatcc ttttataaag
     7021 gaatccggtg cgcgcgccga gatctggtct tatggcattc gtaatccgca aggaatggcg
     7081 atgaatccgt ggagtaatgc actgtggctg aatgaacatg gcccgcgcgg tggtgatgaa
     7141 attaatatcc cgcaaaaagg caaaaactac ggctggccgc tggcaacctg gggaatcaac
     7201 tattcaggct ttaagatacc ggaagcgaaa ggggagatcg tcgccgggac cgagcaacct
     7261 gttttttact ggaaagattc gcccgccgtg agcggcatgg ccttctataa cagcgataaa
     7321 ttcccccagt ggcagcaaaa attatttatt ggcgcgctga aagataaaga tgtcattgtg
     7381 atgagcgtca acggcgacaa agtaacagaa gatggccgta ttttaacgga cagagggcag
     7441 cgaattcgtg atgttcgcac tggacccgac ggttatttat acgttctcac cgacgagtcc
     7501 agtggggaat tacttaaagt tagcccacgc aattagctaa cgggaatcat caccactttg
     7561 cgcactgcgg ggcgttcagt gagttgctga taccagcgtt gcagatttgg acgcggtgtc
     7621 caggtcaggc caacattaaa caaattgtag atgaacggtg cgatagcgat atcgcccaca
     7681 ccaaactcgt cgccagagaa ccattttact tttgccagtt ccgcatcgag cagggcaaac
     7741 agggcgtcgc actctttgca actggcatca atggcggcct gatcgcgctc ttccggtggt
     7801 gttctgacta atcccatcag gatcccgcga tgagcattac tgagtgtctg gtttgcccag
     7861 tccatccatt tttctgcttc cgcacgacgt gccggtgagt cgatccacag gcgtttttgc
     7921 ccgtactgtg ccgccagata gcggacaatg gcgtttgatt cccaaagaat gagatcactt
     7981 tcgtcgtcac gcaacaacgg caccagcccg ttagggttca tcgccagaaa atcagcatcg
     8041 tgatttatcc caaactcacg gcccgcgaga atttgctcat aaggtagttc cagttcttcg
     8101 agcgtcagca atactttttt tacgttggtt gaattattcc gaccccacag cgtaatcata
     8161 ctcacccctt ttccgacagg cagccacagg ctgaacagga ctccgatggt gaagtaaagt
     8221 taacttttaa gcaacagctg gcaaaaaaat gacaccagaa gcagcgaagc ggcaggatat
     8281 tgcataaact ttaaaaactt taccaactta cggtttcttt aagtttgtgt gtgcgttatt
     8341 aatcaccgaa cttatcatac ggcgatataa cgtatttttt tgaatggata ctcgggtggc
     8401 atttatgacg caatactcct ctctccttcg tggtcttgca gcgggttctg catttttatt
     8461 cctttttgcc ccaacggcat tcgcggccga acaaaccgtt gaagcgccga gcgtggatgc
     8521 gcgtgcatgg attttaatgg attacgccag cggtaaagtg ctggcagaag gcaatgcgga
     8581 tgagaaactg gatcccgcga gcctgactaa aatcatgacc agctatgtgg ttgggcaggc
     8641 gcttaaggcc gataagatta aactcaccga tatggtgacg gtcggtaaag atgcctgggc
     8701 gacgggaaat ccggcactgc gtggttcatc ggtaatgttc ctcaaaccgg gcgatcaggt
     8761 ttcggtggca gacttgaaca aaggtgtgat tatccagtcc ggtaatgacg cctgtattgc
     8821 gctggccgat tacgttgccg ggagccagga gtcatttatt ggtttgatga atggttatgc
     8881 caaaaaactg gggctgacca acactacctt ccagacggtg cacggtctgg atgcgccggg
     8941 gcagttcagt accgcgcgcg atatggcatt gctgggtaaa gcattgatcc acgatgtgcc
     9001 ggaagagtac gccattcata aagagaaaga gttcaccttc aacaaaattc gtcagcctaa
     9061 ccgtaaccgt ctgctgtgga gcagcaatct gaatgttgat ggcatgaaga caggaaccac
     9121 ggcaggcgcg ggatataatc tggttgcttc ggctacccag ggcgatatgc gtttaatctc
     9181 cgtagtgctg ggggcgaaaa ccgaccgtat ccgttttaat gagtctgaga aattattgac
     9241 ctggggtttc cgcttctttg aaaccgtgac gccaattaaa cctgatgcca cctttgtgac
     9301 tcagcgcgtc tggtttggtg ataagagcga agtgaatctc ggggcaggcg aagcgggctc
     9361 agtgaccata ccgcgtgggc agctgaaaaa cctgaaggcg agctacacgt taacggaacc
     9421 gcagcttacc gcaccgctga aaaaaggtca ggttgtcggg accattgatt tccagcttaa
     9481 cggtaaatcc attgagcagc gtccgctgat cgtgatggaa aatgtggaag agggcggatt
     9541 ctttggtcgg atgtgggatt tcgtgatgat gaaattccat cagtggttcg gcagctggtt
     9601 ctcttaatct tctgataacc ggatggcgcg aaacgtcatc cggttatacg tcattaatac
     9661 atcaacttaa tacgctgcgt ctgcgcgtac ttcacatact catcttccgg gcaacaatcg
     9721 ctcaccacaa tatcaaagcg tttcaggtca cccatgcgcg ccggacgcac cttgccaaat
     9781 ttactgtggt caacaaccag cacatgcttt tgcgccatcg acatggccca gtgttttacc
     9841 ggcaactctt caagattaaa acaggtagcg cctttactga catgcacgcc cgccgcagaa
     9901 taaaaagcga tatccgggca aaaattattc agcgtttgct gaaaatcgat gggtttgaaa
     9961 atggcgttgc tggcgtgaaa ttcaccaccg cacagaaatg cgcggcaatg gggtttttct
    10021 ttcagcgcca gaaaggtatt tagcgaataa caaacggcgg taaaagggat ttcattatca
    10081 atcgcttcaa taatccacgg cgtggtggtg ccacagtcaa aaaagagggt ctgatcgggt
    10141 tctaccagtg tcgcagccag ttttgccgcc cggcgttttt cttccaccag gcgggatttt
    10201 tgatcgctta acaggtaatg gctggcgctg cgcggttcca gaacaatata gccgccgagc
    10261 aaaacgacgg gcgcactgtg gttgttcaga tcgcgacgaa tcgtcatctc cgaaacccca
    10321 agcagggcgg cggcgtcttt aagatgtaac ttatcgctgc gttttaattc ttgcagcagc
    10381 tgcccgatac gctcttcgcg acgtgtttcc ataatccctc tgaatagtta ttgaagcgag
    10441 ccgctcaata ctacactttt tagcagagat cagtcacgca cccagccttt gcggatcggt
    10501 aatgcaaaac agaagcgata ccacgattgc agacgttgat agagtttatg ccccatcgct
    10561 tgccagataa tctgggcact aaggcagcca atcatacctg ccagcaatcc accgagcata
    10621 tccagcggcc agtggacgcc aagataaacg cgcgaccagg caatgacgac ggccagcacc
    10681 attaaaagtg agccggacca caggcgatgc cagcataaaa atgccagtgc aaaggtgaaa
    10741 atcaccgtac cgtgatcgct cgggaatgag tcatccgccg catgatgcag gaagttatag
    10801 ccgatatttt cgacaaacgg tcggtcgtgc ggaaaaagat gtcccatcgt ccaggacacc
    10861 aacaggctga ccgccagcgc gatagcgatt tttatcacca gttgccgttg tgctgtaagc
    10921 ccccacaacc aaagtaccgc ggccagcagc ggcaccacgg taatcaaatc tttagcaata
    10981 aaaatcgcca acgagatcat ccacggagcc gagtctggcg tcgcgttaat aagagagaat
    11041 agagagagat tcaaattttc cagcataact tcccgacgca aagtgattaa aagggagcca
    11101 atacaggcaa gtcgttgaga ataaagtgca ggttaaactg ggtaaagcgg catcgtctta
    11161 tttccctcaa gcggcctgtt tacggtgggt gattgtaacg ggcataggtt aaataaaact
    11221 taaagaaagc gtagctatac tcgcaataat gtaagaatgt gcttaaccgt ggtttcagct
    11281 acaaaattcg ctttctcgtt agctgcgctt ttattaaact ctgcgcgatt attattggcg
    11341 aagaaattgc atgcaaaata aattagcttc cggtgccagg cttggacgtc aggcgttact
    11401 tttccctctc tgtctggtgc tttacgaatt ttcaacctat atcggcaacg atatgattca
    11461 acccggtatg ttggccgtgg tggaacaata tcaggcgggc attgattggg ttcctacttc
    11521 gatgaccgcg tatctggcgg gcgggatgtt tttacaatgg ctcctggggc cgttgtcgga
    11581 tcgtattggt cgccgtccgg tgatgctggc gggtgtggtg tggtttattg tcacctgtct
    11641 ggcaatatta ctggcgcaaa acattgaaca attcaccctg ttgcgcttct tgcagggcat
    11701 aagcctctgt ttcattggcg ctgtgggata cgccgcaatt caggaatcct tcgaagaggc
    11761 ggtttgtatc aagatcaccg cgctgatggc gaacgtggcg ctgattgctc cgctacttgg
    11821 tccactggtg ggcgcggcgt ggatccatgt gctgccctgg gaagggatgt ttgtcttgtt
    11881 tgccgcattg gcagcgatct cctttttcgg tctgcaacga gccatgcctg aaaccgccac
    11941 gcgtataggc gagaaactgt cgctgaaaga actcggtcgt gactataagc tggtgctgaa
    12001 gaacggtcgc tttgtggcgg gggcgctggc gctgggattc gttagcctgc cgttgctggc
    12061 gtggatcgct cagtcgccga ttatcatcat taccggcgag cagttgagca gctatgaata
    12121 tggtttgctg caagtgccta ttttcggggc gttaattgcg ggtaacttgc tgttagcgcg
    12181 tctgacctcg cgccgcaccg tacgttcgct gattattatg ggcggctggc cgattatgat
    12241 tggtctgttg gtcgctgctg cggcaacggt tatctcatcg cacgcgtatt tatggatgac
    12301 cgccgggtta agtatttatg ctttcggtat tggtctggcg aatgcgggac tggtgcgatt
    12361 aaccctgttt gccagcgata tgagtaaagg tacggtttct gcggcgatgg gaatgctgca
    12421 aatgctgatc tttaccgtcg gtattgaaat cagcaaacat gcctggctga acgggggcaa
    12481 cggactgttt aatctcttca accttgtcaa cggaattttg tggctgtcgc tgatggttat
    12541 ctttttaaaa gataaacaga tgggaaattc tcacgaaggg taaaaaaatg cctgattgct
    12601 ttgtgcgatc aggcattctc gaattaatgg tgatggtcgt caatctggtg ttcgataacc
    12661 atcccttcac ctacgctggc aagatggcga acataaggat gcgggtggta agccggagct
    12721 ggcgcaggag ccacatagac ggtttgcggc acagtcgcga cgctgaccgc ttgtggaacg
    12781 ctgactgaat caggaaccac aaccactttg tatccactcg gcacatcgac ggtgacactt
    12841 tgcgccatcg ccacaccagt aaagcccatt aaaagcgcgc ccaacagtag acaatttttc
    12901 ataatcattt cgcctgaaat aatgcagcgg gttaatttcc gcgaattatg cagccttcat
    12961 tcaggtattg tgttgccggg cgtgctgccg tttttattgg tcaaatggcg cttcgtgttt
    13021 aagaacttta tcgatcacat ccagtacgcc ttcacggtta ttggagcctg cccggtattt
    13081 tgccgctgcg acgaccgcgt tgccggcatt ttccattgca aaactaaagc ctgcctgacg
    13141 cagcatctca atatcgttac cgccatcgcc aaagaccacc acttcgctgt cgtctattcc
    13201 ccataatttc tgcagttggc gaaggccatt ggctttatgt acgccgggga taatcagatc
    13261 gatgctgccg ttgccggtgt ggaccggcac cataatatcg ccgatggcct catgtaatgt
    13321 tttttgtact tgtggaatca gttcatcgga aagattcagg ccaaacttaa agaagatatc
    13381 ttctaagttg tcaaagttat cgacgtattc cagacggtga taatacattt ccgccaccat
    13441 tttcatggca tcgtcatatt ttttgagcgt ataggcgcta ttttttccgc aggcaataat
    13501 ttccacttcc gggcgcgtca gcaaatgttc cacgacagta gtaaacgcat ccttcgacag
    13561 ctcgccatta aaaacatctt tgccttcact cactacccag ccgccgtttt cagccacaaa
    13621 ggcaatttca ttagcaattt cagggaagaa agagatcaac tgataatatt gattcccgct
    13681 ggcgaccaca aagcgaatgc cttgtgcttt catttgctga tactgagcca taaaccgctc
    13741 acggttatag gttttttgat cgcttaagaa agtaccatcc atgtctaccg caattaattt
    13801 aatgctcatc aactattctc catcgccgtc tgcgttttgg tatcgggttt ggcgacggct
    13861 ttcgcgacaa tagccgcgag aataaccagc gccagtacaa ccagcattgc actacgtaat
    13921 ccataatgtt cgccgagata gcccagcagc ggcggcccga cgaggaaagc cagataaccg
    13981 gtcgttgcta ccacactgac gcgggttggt gcatcggggc cggtatcgct ggcggcagaa
    14041 atggtcagcg ggaagcccag cgaggcaccc agtccccaga gtacaacaga caccccagcg
    14101 acccaggcgc tatcgacaaa aataatcagc ccaataccca acgcccccat tagcgcactg
    14161 gcccgaacca cggcaacgcg actgtaacgg tcgatgaacc aaccgccagt gaagcgtcca
    14221 acggtcatcc ccagggtaaa accggcataa atcagcgagc cggaagtggg gctaaaaccg
    14281 tgaccgtcaa ccattaataa gggtaaccag tcgttggcag aaccttcggc aaaggccatc
    14341 gccagcacca caacaccaat cagcagcaac tggatatcgc gataaaaagg tacgcctttt
    14401 tcgccatgct gggtgccatc ggcagcattt ttgcccgtac cgtcagggat tgcctgaatg
    14461 gcgatatata taggcgcgat gcctaccagc gccgccaata aaatgtgcac cgttgccgga
    14521 acgccaaagg ccgtcagtgc catcccgaca ccagcgcctg ccagcgtgcc caggctataa
    14581 aaaccgtgca tcatcggcaa aaccgtttta ttcatttctc gctcaacggc ggcaccttca
    14641 acgtttatcg ccacttccgc agaaccaaaa cttgccccaa agacgccgag accaacggca
    14701 aagagcaggg gcgatgtcag ccagagtgcc agacttaata tcatcatccc gaccaatgcg
    14761 caggacatgg tgaccaggat gacattacgt gtcccaaagc gtttcactaa ccacgccgag
    14821 cagagaatac cgctcatcga accgatcgac agaccaaaga gaacaccgcc catttcagcg
    14881 atcgagacag agagaatatc gcggatagca ggcgtacggg ttgcccagga cgccattaac
    14941 aggcccggca aaaagaagaa cataaacagc gcccaggttc ggcgtttcaa tgcattacgt
    15001 gaagaattta cggtcataga tcacgtcaaa ataagaagag ggaagacaac attagcaagg
    15061 ttgtgtacat ttgtacacaa ttgcagacag aggaaatgac atgcgtcgcg ctaacgatcc
    15121 gcaacggcga gaaaaaatta tccaggccac gctggaggcg gtgaaacttt acggaataca
    15181 tgctgttacg caccgcaaaa ttgctaccct tgccggggta ccgttgggat cgatgaccta
    15241 ctatttttca ggaattgatg agttgttgct ggaggcgttc agcagtttta ctgagatcat
    15301 gtcccggcaa tatcaggcat tttttagcga tgttagtgat gctcaaggcg catgccaggc
    15361 tatcaccgat atgatctaca gctcacaggt tgcaacgccg gataacatgg agctgatgta
    15421 ccagctctac gcgctggcta gccgaaaacc gctattaaaa acggtaatgc aaaactggat
    15481 gcagcgcagt cagcaaacgc tcgaacaatg gtttgaaccc ggaaccgccc gcgcgcttga
    15541 tgcgtttatt gaggggatga cgctgcattt tgtcaccgac cgtaagccgc tatcgcgcga
    15601 ggagattttg aggatggttg agagggttgc agggtagtag ataaatttca ggcaacaaaa
    15661 aacccatcaa ccttgaaccg aaatggcggg gttgatgggc tccacaaaat ggggacatca
    15721 aagaaaagca gtggcactaa ttaagactga tgccctgcgg aaaagttctg cggttgtgca
    15781 aaaaaatttc attttcaggg caacttcagt tttatcctaa tcctggccat accatgacga
    15841 tgattgtccc tgccagcgtc agcaggacgt tggcgattgc gtaggtgccc gcatagccca
    15901 gcgccgggat gttactgcga gctgtatcac tgatgatctc cattgccggc gcgcaggtgc
    15961 gtgcgcccat cattgcgccg aacaacagtg cgcggttcat tcgcaataca taagcaccga
    16021 acaagaaaca gataaccacg ggcacaagac tgacaatcaa tccggcaatc aacatctgac
    16081 cgccaatcgc gcccagaccg ttattaatac cgctaccggc gctcagacca acgcctgcca
    16141 taaacaccat caagccgaac tctttcacca tgcttaatgc cccttgcgga atgtaaccga
    16201 aggtcgggtg gttagcacgc ataaagccca gcataattcc ggcgaataac aacccggcag
    16261 cgttccccat gccgaaactg aatgtgctga actggaaggt gatcatcccg atcatcagcc
    16321 caataacaaa gaaggcgcag aatgccagca ggtcagtgac ctggctgtga atcgagataa
    16381 agccgatgcg atcggcgatg gtttttacgc ggcgggcatc gccgctgact tgtaaaacgt
    16441 cacctttgtt aagcacgacg ttgtcatcaa tcggcatctc aatctggcta cgaatgacgc
    16501 ggttaaggaa gcaaccgtga tcggtcaact tcagttgtgc gagacgttta cctacagcgt
    16561 tatggttttt aacgaccact tcttcagtga cgatacgcat gtcgagaagg tcacgatcga
    16621 aaacttcttt accgttacgg aagctgggat cgagtcgggc atgggcgtcg ggatagccta
    16681 ccaacgctat ttcatcgccc atttgtagca cggcatcacc gtctggattt gccagaatcc
    16741 cgttacgtcg aatacgttca atgtagcagc cggtttgtcg ataaataccc agttcacgca
    16801 gatttttgcc gtcggtccag gccaccagct ccgggccgac gcgataggcg cggatcaccg
    16861 gtaaataaac cttacggttg gcatcagtgt ccaggccacg ttcgcgggcg atttgctggg
    16921 cgctggtctg taagtcctga tgctgcaatt tcggcaagta acgcgcacca acaatcaaac
    16981 tcaccagacc gattaaatag gttaaggcat acccgaggct cagattatcc agtgccagtg
    17041 agagctgcct gctttccata ccggaatgac gcagtgtatc gccagcaccg accagaaccg
    17101 gtgtcgacgt catagagcct gctaacatac cggccgtcag gccaatatcc cagccaaaca
    17161 gcttacctaa ccctaaggcg atcaccagcg cactgccaac catcaccagt gctaacatta
    17221 ggtaattttt cccatcgcga aaaaaaatgg aaaaaaagtt cggtccggct tcgaccccga
    17281 cgcagaaaat aaacagcata aagccaagat taagcgcatc ggtgttaatg ctgaaatgtt
    17341 gttggcctaa taacagcgat acgactaaaa cgccaatgga attacccagt tggatcgaac
    17401 caagtcgtaa ctttccgaga catagcccaa gc
//
LOCUS       DBFIFI010000083        17389 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.083, whole genome shotgun sequence.
ACCESSION   DBFIFI010000083 DBFIFI010000000
VERSION     DBFIFI010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.083"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(43..774)
                     /gene="rlmB"
                     /locus_tag="N7B39_002653"
     CDS             complement(43..774)
                     /gene="rlmB"
                     /locus_tag="N7B39_002653"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HGU2422608.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(955..3396)
                     /gene="rnr"
                     /locus_tag="N7B39_002654"
     CDS             complement(955..3396)
                     /gene="rnr"
                     /locus_tag="N7B39_002654"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HGU2422609.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLMLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVSDWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3435..3860)
                     /gene="nsrR"
                     /locus_tag="N7B39_002655"
     CDS             complement(3435..3860)
                     /gene="nsrR"
                     /locus_tag="N7B39_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HGU2422610.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGLMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4065..5363)
                     /gene="purA"
                     /locus_tag="N7B39_002656"
     CDS             complement(4065..5363)
                     /gene="purA"
                     /locus_tag="N7B39_002656"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HGU2422611.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5467..5664)
                     /gene="yjeT"
                     /locus_tag="N7B39_002657"
     CDS             complement(5467..5664)
                     /gene="yjeT"
                     /locus_tag="N7B39_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HGU2422612.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5746..6750)
                     /gene="hflC"
                     /locus_tag="N7B39_002658"
     CDS             complement(5746..6750)
                     /gene="hflC"
                     /locus_tag="N7B39_002658"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HGU2422613.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6753..8012)
                     /gene="hflK"
                     /locus_tag="N7B39_002659"
     CDS             complement(6753..8012)
                     /gene="hflK"
                     /locus_tag="N7B39_002659"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HGU2422614.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8098..9378)
                     /gene="hflX"
                     /locus_tag="N7B39_002660"
     CDS             complement(8098..9378)
                     /gene="hflX"
                     /locus_tag="N7B39_002660"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HGU2422615.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9455..9763)
                     /gene="hfq"
                     /locus_tag="N7B39_002661"
     CDS             complement(9455..9763)
                     /gene="hfq"
                     /locus_tag="N7B39_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HGU2422616.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9849..10799)
                     /gene="miaA"
                     /locus_tag="N7B39_002662"
     CDS             complement(9849..10799)
                     /gene="miaA"
                     /locus_tag="N7B39_002662"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HGU2422617.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEIDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLGGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10792..12639)
                     /gene="mutL"
                     /locus_tag="N7B39_002663"
     CDS             complement(10792..12639)
                     /gene="mutL"
                     /locus_tag="N7B39_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HGU2422618.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRPIPENRVAAGRNHFAEPAVREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQVQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12649..13986)
                     /gene="amiB"
                     /locus_tag="N7B39_002664"
     CDS             complement(12649..13986)
                     /gene="amiB"
                     /locus_tag="N7B39_002664"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HGU2422619.1"
                     /translation="MMYRIRNWLVATLLLLCAQVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADAPPPPPPPPVVVKRVETPTVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANNGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14005..14466)
                     /gene="tsaE"
                     /locus_tag="N7B39_002665"
     CDS             complement(14005..14466)
                     /gene="tsaE"
                     /locus_tag="N7B39_002665"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HGU2422620.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14438..15985)
                     /gene="nnr"
                     /locus_tag="N7B39_002666"
     CDS             complement(14438..15985)
                     /gene="nnr"
                     /locus_tag="N7B39_002666"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HGU2422621.1"
                     /translation="MTDHTMKKNPVSIPHTVWHADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVALIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15984..17123
                     /gene="queG"
                     /locus_tag="N7B39_002667"
     CDS             15984..17123
                     /gene="queG"
                     /locus_tag="N7B39_002667"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HGU2422622.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKRLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAMAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17106..17159)
                     /locus_tag="N7B39_002668"
     CDS             complement(17106..17159)
                     /locus_tag="N7B39_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2422623.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3937 a         4967 c         4371 g         4114 t
ORIGIN      
        1 aaaaccatcc aaatctggat ggcttttcat aattcagagg aactagctgc gctgacgaac
       61 cgcttcaaat aagcaaattc cggttgcaac cgaaacgttc agggaagaaa cacttcctgc
      121 cattgggatg ctgatcaact catcgcaatg ttcacgggtc aggcgacgca taccttcacc
      181 ttccgcgccc atcaccagcg ccaggcgtcc ggtcattttg ctttgataga gcgtatgatc
      241 cgcctcacct gccgtaccga cgatccagat attctcttcc tgcaacatac gcatggtgcg
      301 cgcaaggtta gtcacccgaa tcagtggaac gctttctgcc gcgccgcagg ctactttttt
      361 cgccgtggcg ttgagctgtg cggagcgatc tttcggcaca atcaccgcgt gaacgccagc
      421 agcgtccgcg ctacgcaggc acgcgccgag gttgtgcgga tcggttacac cgtcgaggat
      481 cagcaggaac ggttgatcga gcgaagcgat cagatccggc agatcgtttt cctggtactg
      541 acgtcctggc ttcacgcggg cgataatgcc ctgatgcacg gcaccgtcgc ttttctcgtc
      601 gagatattgg cggtttgcca actggataac cacgccctgg gactcaaggg cgtggatcag
      661 cggtaacaga cgtttatctt cacggccttt taaaataaag acttcctgaa aacgttcagg
      721 ggcgcgctcc agcagggcct gcactgcatg gatgccgtaa atcatttcgc tcattaatgt
      781 actcgttgtt tacgttttgc ctgatgcact acgtttatcc ggtctacatg atccctgcaa
      841 tatattgaat ttgcgtgatt atgtaggccg gataaggcgt tcacgccgca tccggcatga
      901 acaaagcgca ttttgtcagc aatctaaccc tcttctgttt gaagagggta atgatcactc
      961 tgccactttt ttcttcgccg cacgcttcgc tttggtcgct gcagctattt tctgcgtttt
     1021 cgccgatggc tttttcgctt ttctcgcgtc tttcttcgct gctttcggct tcgttttttt
     1081 ctcaccgcgg aaggcgctgt ctggctcaaa gtttaccttt ttaccgacct gacgacgctt
     1141 gccgcctttt ttacctgcat cgcctttttt cgctttctcg cgcgccgttt taccgacgtt
     1201 gcgcggtgcg cgttcgctgg agatcaggct aaagtcgatt ttgcgctcgt ccatattaac
     1261 cgcttcgacg cgaacttcca cgcgatcgcc caggcgataa gtctggccgc tggattcacc
     1321 catcaggcgt tgccctacct ggtcaaagcg atagtagtca ttgtccagcg aagagacatg
     1381 gaccagccca tcaatgaaca agtcgtccag acggacgaag aagccaaagc cagtgacgct
     1441 ggaaattacg cctttaaaga cgttacctac ctggtcgagc atgaagtcac acttcagcca
     1501 gtcagacaca tcgcgcgttg cttcgtcggc acgacgttcc gccatcgaac agtgctgacc
     1561 caattgcagc atctcttcca tcgaataatg gtagccaccg gtttcagtgg tgttgccctg
     1621 atgcccctgc tctttcgcca gcagatactt aatggcgcgg tgcagcatca ggtcaggata
     1681 acgacgaatc ggcgaagtaa agtgcgcata ggactgcaat gccaggccaa agtgaccacg
     1741 gttttccgga tcgtaaatcg cctgtttcat cgagcgcagc agcatggttt gcagcatttc
     1801 tgcatcagga cggtcggcaa ccgactccag cagctccgcg tagtcgcgcg gttccggctt
     1861 gttaccgccc ggcagttcca gccccagctc tgccagcact gaacggaaag aggtaatcgc
     1921 ttcggtgctc ggcttgtcgt gaatacggaa cagtgccggt tctttcgctt tctcaacgaa
     1981 acgcgccgcc gagatattcg ccagaatcat gcactcttca attaacttgt gtgcgtcgtt
     2041 acgctgagtc tgttcgatac gttcaatacg gcgttcagcg ttgaaaatga acttcgcttc
     2101 ttcgctctca aatgagatcc cgccgcgttc ttcacgggct ttatccagca ctttatagag
     2161 gttatgcaac tcttcgagat gcttaaccag cggggcgtac tgctcgcgca gatcctgatc
     2221 gccctgcaga atatgccaga ctttggtgta ggtcagacgc gcgtgagagc tcatcaccgc
     2281 ttcgtagaat ttgtagccag tcaggcggcc tttcgacgaa accgtcatct cgcacaccat
     2341 acacaggcgg tctacctgcg gattgagcga gcacagaccg ttagagagca cttccggcag
     2401 catcgggata acctgcgaag ggaagtacac cgacgtacca cggttacgcg cttctctgtc
     2461 cagcggcgtt ggcggacgca catagtagct gacgtcagca atcgcgaccc ataaacgcca
     2521 gccgccgccg cgttttttct cgcagtaaac cgcatcgtca aagtcacggg cgtcttcgcc
     2581 atcaatggtg accagcggta aatcgcgcaa atcgacacgg cccgcttttg cttcttccgg
     2641 cacttcttct ttcagcccag caacctgttg ctcaacagcc tgcggccaga tgtacggaat
     2701 ttcatgggta cgcagagcga tatcaaccgc catgcccgtg cccatattgt cgcccagcac
     2761 ttcgacgatt ttacccaccg ctttggtacg gcgagtcggg cgttgggtca gttcaacaac
     2821 caccacaaag cccatccgcg cgcccatgat ctgatcgggc gggattaaga tatcgaagct
     2881 cagacggcta tcgtcaggaa ccacaaagcc gacgcccgct tcggtaaagt agcgaccaac
     2941 aatctggctg gtttttggca ccagtacgcg gacaatacgc gcttcacgac gacctttacg
     3001 gtcagcaccc agcggctgag ccagcacctg atcgccatga atgcaggttt tcatctgctc
     3061 gctggagaga tacaaatcat ctttacgccc ttcaacccgc agaaagccgt agccatcacg
     3121 gtggccaata acggtacctt tcaccaggtc gaggcgttcc ggcagcgcat agcactgacg
     3181 acgagtaaag accagttgac catcgcgctc catcgcgcgc aggcggcgac gcaggccttc
     3241 aagctgctct tcgccttcaa tgtgcagttc taccgccagc tcatcacggc tggccggttt
     3301 ttcacgtttg gttaaatgtt cgaggataaa ttcccgacta gggatgggat tcgcgtattt
     3361 ttcagcttcg cgttcctgga aaggatcttg tgacatctcg gttcctccgt tgtcatctct
     3421 gatgaagatt ttcgtcactc caccagcaat aatttataaa gcggttgatt ctcttcaacc
     3481 aaatcggcaa gcgtgtagtt atccagttcc gtaagaaaac tttgcacggc cttagaaagc
     3541 gcctgtttca accgacaggc aggtgtaatg tggcaaaact cactgctgca attcaccagc
     3601 gataagggct ccagctcgcg caccacatca ccaatacgta tcgcactcgc cggtttaccc
     3661 aggcgaatgc cgccattttt tccacgaaca gcagtcacgt agccggcacg actaagttga
     3721 ttgattattt tgaccatatg attacgggag acgccgtaga cgtcagtcac ttcagaaata
     3781 ctggtcatca gcccttctgg caatgacgcc atgtagatca gcgcacgtaa tccgtaatca
     3841 gtgaaactcg ttaactgcac atcaacctca aaagggaaat cgggaaaaaa tacatttata
     3901 ttgatgataa accagccaca agctgtgtcg ctaatttatt tcagttcggg gaaggaaaaa
     3961 agcgaggatt taacactgtg tcggatagcg taatggctta accgacacag caatgtatca
     4021 cactgttagc ccggcaggca aaatatctgc caggcgtacc agaattacgc gtcgaacggg
     4081 tcgcgcagaa tcatggtttc agtacgatcc ggaccggtag agatgatatc gatcggtaca
     4141 ccggtcagct cttcaatgcg cttgatgtag ttcagcgccg cctgcggcag gccgctacga
     4201 tctttcacgc cgaaggtgga ttcagaccag cccggcatgg tttcgtaaat cggctctaca
     4261 cctttccagt cgtcagctgc cagcggagtg gtagtcactt cacgaccatc cggcatacgg
     4321 taagccacac agagtttcac ctctttcagg ccatccagaa cgtccagttt agtcaggcag
     4381 aagccagaca gggagttcag ctgtaccgca cgacgaacgg caacggtgtc cagccagccg
     4441 gtacgacgac gacgacccgt agttgcgccg aattcgttac cctgcttgca gaggaactcg
     4501 ccagtttcat caaacagttc agtcgggaac ggacctgcac ccacacgagt ggagtaagct
     4561 ttgaggatac ccagaacgta atcaacataa cgcgggccca ggccggaacc ggtcgccacg
     4621 ccaccagcag tggtgttgga agaagttacg tacggataag taccgtggtc gatatccagc
     4681 agcgtaccct gcgcaccttc gaacatgacg aaatcgccac gctgacgcgc ctggtcgagc
     4741 aggtcagaaa cgtcaaccac catagaagtc aggatgtcgg caacagccat cgtatcatcc
     4801 agaactttct ggtaatcaac cgcttcagct ttgtagtagt taaccaactg gaagttgtga
     4861 tattccatca cttctttcag tttttcagcg aaggtttctt tgtcgaaaag gtcgccaaca
     4921 cgcagaccgc gacgtgccac tttatcttca taagcaggcc cgataccacg accggtggtg
     4981 ccgatcgctt tcgcgccacg cgctttctca cgcgcgttat ccagcgcaac atgataatca
     5041 aggatcagcg gacatgcttc agacagcagc agacgctcac gaacggggat gccacggtct
     5101 tccagttctt tcatctcttt catcagcgcg gccggagaca gcacaacacc gttaccgatg
     5161 atgctagtta cattctcgcg gagaatacct gatggaataa gatggagaac ggttttttca
     5221 ccgttgatta cgagagtatg gcctgcgttg tgaccgccct ggtagcgtac aacatattta
     5281 gcccgttcag tcagaagatc gacgatctta cctttacctt cgtcacccca ttgggtgccc
     5341 agtacgacga cgttgttacc catttttcaa aatcaccgtt tgcttaaaaa tggattctac
     5401 catcgctttt tcagagttac agcacttttt gcactcaaaa atgacctcga tcagtctgtt
     5461 ttttgttcag ccaatcgttt tcctcaacat gtagtagacc acaacgcccg caaccacaag
     5521 tccaccgcca aaacgacgta aaatattatc gggcaaattg gtcatcgcag agatcatctt
     5581 cttccatgcc ttcgggtaaa gcatcggccc taaaccttcc agtaccaaaa ccagggcaag
     5641 cgccagccag attgtcgaat tcattcatta tccttataga aaaagaaaac caccgacatc
     5701 cctgaggatg cggtggcttt attgacctgt accgcagtcg ttatattaac gcgttgcgga
     5761 agtcggcgtc ttcatgtagc ggaagaaatc gctatccggg ctcatgacca tcacgtcctg
     5821 attgccagag aagctgttct cataagcacg caggctacgg atgaatgcgt agaagtccgg
     5881 atctttactg aatgcatcag caaacagttt ggctgcttcg gcatcgcctt caccgcgcat
     5941 gatgcggccc tgacgctcag cttctgccag cgttctggtc acttcatagt cggcagtcgc
     6001 gcgcagtttt tccgcttctt cctgaccttg tgaacggtga cgacgcgcta ccgcttcacg
     6061 ctcggcgcgc atacggttgt agatcgcttc agacacttcg gtcggcaggt tgatctgctt
     6121 aattcgcaca tcgacaactt caatacccag cgccgccata ctgttcgggt tgatgaccgg
     6181 aactttgccc ttcgtctcag ccgttacgcg ctctgccgct tcggcaatgg cgttatctgc
     6241 cgccggggta gtaacttcat cttctgtacc cgcagaaccg gagttcagcg cgtcacgtac
     6301 ttcgagggtc agacgaccac gggaatcggt gacgatgtct ttcacgtcca ggcgaccaat
     6361 ttcagaacgc agacggtcag agaacttacg tttcaacagc acttccgctt gcgaaatgtc
     6421 gccccccccc gttgccaggt agtaacggct gaaatcgctg atgcgccatt tgatgtaaga
     6481 gtcgacgatc aggtctttct tctctttggt cacaaagcgg tcggcctggt tgtccatggt
     6541 ctgaatacgt gcgtcgagca ttttcaccgt ttcaatgaac ggtatcttga aatgcagacc
     6601 cggctcataa accagaggtt tgttgtcatc gtcacgcagt accttaccaa aacgcagcgt
     6661 aataccgcgc tcaccttctt tgacgacaaa gacagacatg taaagcacta ccagcacgat
     6721 gatgataatc gcgataactg acttacgcat cgttattccc cctgacgctg gtagtcgtta
     6781 cgctgcgcat tggcgcggcg ttggtccata atatcgccct gactggtgga cgacgtgttg
     6841 cttgctccac tggttgtgga ggaagaggct ggcggcagac gcagcagatt gctggcaccg
     6901 ttatcgctct tcgccgcagg ggcgttacca cctttcagca tctggtctaa cggcagaacc
     6961 atcaggttgc cacctttatc gttaaccagc actttgcggg tgttacccaa cactttttcc
     7021 atcgtctcga tatacagacg ctcgcgagta atttccggcg cggctttata ttccggcaga
     7081 agtttagcaa aacgcgccac ttcaccctga gcttccagga tggtctgggc cttgtacgca
     7141 cgcgcctctt cgaggatacg ttgcgcctga ccgttcgcac gcggctgaac ttcgttggta
     7201 tacgcttctg cttcacgaat gtattgctgt tcgttttcac gcgcggcaat cgcatcgtca
     7261 aacgccgctt ttacttcttc cggcggacga gcagcctgga agttgacgtc cagcagcgtg
     7321 atacccatgt catacggacg aatcgtctct tccagttcgc gctgagtatc gctacgaatc
     7381 acggtacgac cttccgtcag aatgcggtcc atggtgtatt taccgataac tccacgcagg
     7441 gcgctgtcgg tagcctgacg caggctgtca tccgggctgg tcacgctata cagatatttt
     7501 tccggattgg tgacgcggta ctgcacgttc atctcaacgc gcactacgtt ctcgtccgac
     7561 gtcagcatca caccagaagc tgccagttca cgcacggctt ccacgttcac cggtttgact
     7621 tcgtcgataa acgtcggttt ccagttcaga cctggctcaa ccagatggct gaatttacca
     7681 aagcgtgtta ccacgccgcg ttcggcttct ttaatggtat agaaaccact ggccgcccag
     7741 ataatgacaa tcgctgccgc tgcgatggta acgacacgac cgccaagctg cgggcgcggg
     7801 ccttgcgatg aactgccacc gccagatccg gtgcctttac cgccgcccag accaccgagc
     7861 tttttgctca gtttgcggaa gatatcatct aaatcaggtg gcccttgatc gcgaccgcct
     7921 ttgtttccat ttccctcaga gttgccgcca ggtttgctgc ttccccacgg gtcgcggtct
     7981 tgtccgttat taccgggctg attccacgcc atgtttgtgc tccatatttg ttatgcggtg
     8041 atccctgttg tctttcgacg ccagaggata tgactccacg cttcagacgc tacgccgtta
     8101 gatcaggtaa tcgatcaacg ccggttcttg tttacagagg cgacgccagt caacgatcgg
     8161 catacgaact tgcagactta cgctgccgtc ctcctccatc cactcttttt ctattgcctg
     8221 aagctgataa aaacgacttc tcagacgccc ttcctgcggt ggcagacgca atgtatgctg
     8281 cgccacctcg ccggaaagcc gctccgtcaa agcctgaaaa agctgtggta tccccgctcc
     8341 ggtctgtgcg gaaagccaga cacggatcgg tttgttctct tcgtcccgat caatacgcgg
     8401 ttcgaaatct tccagcatat cgatcttgtt catcaccagc agggttggga tctcgtgagc
     8461 gtcgatctct tcaagaaccg tattcaccgc ttcgatgttt tcttgtacac gcacatccgc
     8521 cgcatcaatc acatgcagca gcaatgtggc ttgccgcgtc tcttgtaacg tggctttaaa
     8581 tgctgccacc agatcgtgcg gcaggtggcg aataaaccct acggtatctg caagtacggt
     8641 ttcaccgaca tctgcaacgt caatacgccg caacgtcggg tcgagggtgg caaacaactg
     8701 gtctgccgcg tagacccgcg cttcggtgat gcgattgaaa agggtagatt taccggcgtt
     8761 ggtatatccc accagcgaaa cagtaggaac gtcggctttg atacgcgatt gccgcccctg
     8821 ctcacgctgc ttttcaactc tttccaggcg cgactgtatc tgcacgatgc gattacgcaa
     8881 caaacgacgg tcggtttcga gctgggtttc acccggacca cgcaaaccaa tcccgccttt
     8941 ctgtctttca aggtgggtcc agccacgcac caggcgtgta gccagatggc gcagctgcgc
     9001 cagctcaacc tgcaacttac cctcatgggt acgcgcacgt tgggcgaaaa tatctaaaat
     9061 aaggccggtg cggtcgataa cacgacactc gcacaaacgc tccaggttac gctcttgcgc
     9121 cgggctcagg gcatggtcaa aaagaacgac cgaagcaccc gtcgctttga cagcttccgc
     9181 aatttcaact gctttacctt cacctacaaa atactttggg tgcggcgctt tacggctacc
     9241 ggtaatcacc tgcaatgctt cgacaccggc ggaagagacc agagattcaa actcctggag
     9301 gtcttccata tctttgtctt gcgtaaaata gatgtgtacc agtaccgcct gctcaccagc
     9361 atcataacgg tcaaacaagc gtataaccct ctaaatagat cagcggggaa cgcaggatcg
     9421 ctggctcccc gtgtaaaaaa aacagcccga aaccttattc ggtttcttcg ctgtcctgtt
     9481 gcgcggaagt attctgcgcg ctgctaccat gatggtagtt actgctggta ccgccaccgg
     9541 cgttgttact gtgatgagaa accgggcgag acgggacaac agtagaaatc gcgtgcttgt
     9601 aaaccatctg gctgaccgtg tttttcaaca ggatcacgaa ctgatcaaaa gactcgattt
     9661 gcccttgcag cttaatacca ttcaccaaat aaatagaaac tggaacacgt tcccgacgca
     9721 gtgcgttcag gaacggatct tgtaaagatt gccccttagc cattctctct tttccttata
     9781 tgcttatttg tactttgaac ctttcgattc tgaaaaaatt gcgcacgata cgtctcaatt
     9841 gtacacattc agcctgcgat agcaccaaca acctgtaata cttcgtcacg cgcctgttct
     9901 ggtttttcac tgtcaagcca gtgaacccct tcccaaccac gcagccaggt tatctgccgc
     9961 ttcgccaact gtctcgtggc gcaaacacct cgataaacca tttcatcgta tgagatttcg
    10021 cctccaaggt aagaccacat ctggcgataa cccacgcaac gaatggaagg caagtccgta
    10081 tgcaaatctc ctcgggcaaa aagcgcccgg acttctgctt caaaacctga agccaacatc
    10141 tgatgaaaac gctgctcaat tcgttgatgg agcagttcac ggctcgccgg ggcgatggcg
    10201 aactgatgca cctgatacgg tagagcgtct cctgacgttt gcgtcagttc cgttaaagtt
    10261 ttacccgaaa tgaaaaaaac ttccagtgcc cgggaaagcc tttgtggatc atttggatga
    10321 atccttgctg ccgcaaccgg atcgatctcc tgaagttgac gatgcaatga ctcccaacct
    10381 tgctctgccg cctgttgctc aattctggcc cgtacttccg ggtctgccga cggtagcggc
    10441 gacaaccctt ccagcaatgc cttgaaatac aacatcgtac cgcccactaa cagtgggatc
    10501 cgccccgccg cggtgatatc ggccatttcc gccagcgcat cgcggcgaaa atcagcagcc
    10561 gagtaagcct gcgacggatc gcgaatatcc agcaatcggt gcggcgcggc gagtaactct
    10621 tcagcgttcg gcttcgccgt cccgatatcc atccctttgt aaataagggc agaatcaacg
    10681 cttatcaact ctactggtaa aattttacgc agctcaatgg ctaacgccgt tttaccggag
    10741 gccgtcggcc ccatcaaaaa aatcgcctta ggcaggctcg ccttactgat atcactcatc
    10801 tttcagggct tttatcgccg gatgtaaatc aacagattgt aacagaccac ccggcggcgt
    10861 tttcacaagt tgcggacata accgttccac gtccgccagc agggttatgg cctgtgccat
    10921 tgaccactgc gcatgttcgc tcatcagatt tcgtgcaatc cactgcgcaa tattgccagg
    10981 ttcgaatacg gactgcttcg ctaggtagcc tatcagttca ggaatcaaga tttgtaaatt
    11041 ttgttggcgt aagggtaaag gcacggccct gatggtcaca tgctgtgcat ctgactggaa
    11101 atcaataccc aattccgcca gggcagactg cgctttttct aatgccgatt tttcttcgcc
    11161 agaaactttt agccgcaacg gaatcagcaa cggctgggcg caaacgggcg cttcacccgg
    11221 cgtcagttgt acctgacgca gccaacgttc tgccactggc aaggctaaaa gtgaaatgtt
    11281 gccgtcgcgc tccagcaacg cacagtcgga atggacgata gtcagtaccc gaccaaaact
    11341 ctgactgttc gccgcaagtg caggttcctg cggttccggc gcttttggtt tttgcatcgg
    11401 cgcgggcgtt tgcaaaagct ggcgatacac ttcaccttgc tgtttctggt aacctggctg
    11461 cgcattcggc cagggggcag ccggacgact acctgatgct ggcgcaggag tgtagcgcgg
    11521 agctactggc tcacgaactg ccggttcagc aaagtgattg cgccccgccg ccacgcggtt
    11581 ttccggaatg ggacgcggtg caggttgggg ttcatcgtcc agcggtagcg gcgtttccag
    11641 ttgctgttgt agcacgctca gcacgccctg atagataaag tcatgcacca gacgcgactg
    11701 atgaaaacgc acttcgtgtt tggcggggtg cacgttgacg tccacctgat gcgggtcgat
    11761 ctccagatac aacacaaatg caggttgctg atcggccccc agtttgtctt cacaggcctg
    11821 gcggatcgcg tgattgatca ggcgatcgcg catcatgcga ccattcacgt agcaatactg
    11881 aatttccgcc agtgcgggcg tggtgtgatt tggatcggcc acccagccgc gcagcgtgag
    11941 atcgccgtgt tgccattcaa tcgccagcgc ttgttcgaga aaagcggtgc cacaaatcgc
    12001 gcctaagcgc cgttcttttt gcccgccttc cggcaccgcg cggtactggc gtacgatttt
    12061 gccgttatgc gacaggttga tcgtgacgtc gaaacgcgcc agtgcgatgc ggcggatgat
    12121 ttcatcgata tggttaaatt cggttttctc ggtgcgcagg aatttgcgcc gcgccggggt
    12181 gttgtagaac aggtccagta cctccagcgt cgttcccacc ggatgcgccg ccggtttaac
    12241 cgtcacgtcc atatcgcgcc cttcggcata ggcctgccag gcttcctgct gttctgcggt
    12301 gcgtgaagtg agcgtcaggc gggaaaccga actgatactc gccagcgcct caccgcgaaa
    12361 gcccaggctg ataatggctt cgagatcgtc cagagaggcg attttactgg tggcatgacg
    12421 cgccagcgcc agcgccagct cgtctttttt gataccacag ccgttatcac gaatgcggat
    12481 aagtttcgcc ccaccgcgtt cgatatcaat atcgatacgc gtcgcacctg catcgaggct
    12541 gttttccacc agttctttga ctaccgacgc aggtcgctcg accacctcac ctgcggcaat
    12601 ctggttcgcc agttgtggcg gtaagacctg aattggcatc aatcgtcctt agtttggcag
    12661 cgtgcgatct ggcgtcgtca ccgtactggc agtttgtgcc gttgcaccct gcggcgcaga
    12721 ttgcatcgga tgcgcaagga aataattgcg caggcctttg taaatggctt ctgccagctg
    12781 ttgttggtaa tcgtcgctcg ccagcaaacg ttcttcgctg ttgttgctga taaaaccggt
    12841 ttcgaccagt actgatggga tatccggcga gcgcagaacg ccaaggctgg cgtgttctgg
    12901 tcgacgttta tgtatttcgc caatgcgttg caactgactg atcatactgg tcgctacatc
    12961 ataccctacc cgctgggaat gaccgaactg taaatccagc accgcctggc ttaaataggg
    13021 gtcagactga ctgttcgcca gcacatcacc cgccccgccc agcagctccg actgtttctc
    13081 gtgctgctcc agccagctgg ccatttcgct gttggcgcga cggttagaga gcacccatac
    13141 ggaagcgcca gtcgcactgc ggttcggtgc ggcatcagcg tgaatcgaca cgaggaaatt
    13201 ggcgttttgc ttacgtgcca catcgctgcg ccccatcacc gagataaagt aatccccgtc
    13261 acgggttaaa acgcctttaa acatcggatc gtcattgagc aaagtacgca atttacgcgc
    13321 gatggcgatg gtgacatttt tctcccgcgt accaccgggg ccgatagcgc cagggtcctg
    13381 accgccgtgt ccggcatcaa tagcgatgat aattttatcg ccattgttag ccgtcgcgcg
    13441 cgctgccgga cgcgttaccg tattactgct gataacaccc gtagtgcggt tactttccgt
    13501 tttaaacgga ttgcgcgccg gttcgctgac gcgcggtgcg acaaccgtag gcgtttcaac
    13561 gcgtttcaca accacaggcg gcggaggagg cggtggcggc gcatcggcgt taatcgtaaa
    13621 gacgacagtg taattgctgc cattctgccg cttcaccgct tcggttttac cattttcggt
    13681 aagatcgacc accagccgta gcgtttgtgc atctttaggc gttccggagc gaatcgcctt
    13741 caccagatta ttaccgctga acaacaacgg cagtccctga atcacgcccg tttgtttgat
    13801 atcgagcgcc acggtgcgtt tgctttgatg gctaaacgca taatcaggat cgccaataaa
    13861 actcaacgtt atccgcgcct gttggttgcc gttagaaacc tgaatatcag agagcgtcgc
    13921 ggcacccacc tgcgcgcaca gcagcagcaa cgtcgctacc aaccaatttc tgatgcgata
    13981 catcatccca ccacctttca aaggttaacc ggctaaacgt gccagcaaca attcacccgc
    14041 agaggaaacc gcactcacgc gcgcctcacg gccttgtgcc tgataatcaa tgtgtatttc
    14101 gacatccggg tcaggaagaa cacctgtacc ttgttgtggc cactccacca ggcagatggc
    14161 atcgttggca aaataatcgc ggatccccat aaactccagc tcctcgggat cggcaaggcg
    14221 gtacaaatca aagtgataaa ccattaaatt gtcgagcgta tagggttcga ccagcgtata
    14281 agtggggctt ttgacattac cctgatggcc caaagcctgt aaaaagcccc ggctaaaggt
    14341 ggttttacct gcgcctaaat cgccatacag atagattacg gttgcgccat cgcaggcttt
    14401 cgctacccgc tcgcccaggt ctaatgttgc ctgctcatca gggagcggaa ttactcgatt
    14461 catcatggtt tttatcagtc acttccgggt taacaatacg ctgtagcgtg gaaaagagat
    14521 cggttgccag catcccgcgc gttccaaaac gcgccgccag tacgtcagct gccgcaccgt
    14581 gcgcgacaca gcctgcacag gctgcatcat acggcgacag tttttgccca agcaatgcgc
    14641 caataatacc agagagcaca tcgcccatgc cgccgcttgc catgcctgca tttccggcat
    14701 caataatgcc taaagcgtca ggatgggcgg cgaccacggt tccggcacct ttcagcaccg
    14761 ctacgccgcc ataacgttgt accagacgtt tggcgcaatg taagcggtca ctttcaattt
    14821 cagcgacgga acagcctaac aaccgtgcgg cctcgccagg atgcggcgtg atcacgcgat
    14881 tgtgacgctt atcgggattg attgccagca ggttcaatgc atcggcatcc cacaacatcg
    14941 gtttgcgaaa attctcaact ttttgcaggg cttttttccc ccactcttgc tggcccagac
    15001 cgggaccaat caccaccaca tcggcccatt ccaggctttc ggtaagagag tccatagtca
    15061 gttcatgcac catcaattct ggtcgtgcag tcagcagcgg cgcaatgttc tcactgcggg
    15121 tcagtactcg gactaaacca gcaccagcac gtagcgccgc ttcccccgtc atacgaatag
    15181 cccccgccgt gccgtgatcg ccaccgataa tcaccagccg cccgtgatcg cctttatgcg
    15241 aagtcgggcg acgcggtttc agccagtgag aaagttgttc tgccgaaaac cgctgaattt
    15301 tcgtctcctg acctgccagc cagctatcca gccccagtga gtcaaaatgc agttgtcctg
    15361 taacatcccg cgcttttcca gtgagcaagc ctggtttcag cgcaataaaa gtgatggtgt
    15421 gatcggcgtt gatcactgcg cctggcgtag cgccggtttc agccagcagg ccggaaggga
    15481 tatcaaccgc cacaatcggc gcaggatggg aattagcgtg gtcgattaac tggctaatgg
    15541 attcgcgggg tgcttgctgc aagccggtac cgagcagcgc atcaacaatc agagctaccg
    15601 actcgggcca gacaatattc gaagcatgga tctcgcctcc cgcgtttaac catgcctcgc
    15661 gtgccagcgc ggcctcttcc ggcaacggtt tatcgctctc ctgggccaac aacgtgacct
    15721 caatgccgac cgctttggcc agtcgcgcga ccacgtagcc atcgccgccg ttattaccat
    15781 gaccgcacaa caccagccag tggcgggcgt caggatacgc cgaacgacac acctggaatg
    15841 cggcctcgcc agcgcgaagc atcagctcat agagtgtgag ccccagcgca tctgccgcct
    15901 cgcgttctcc gcggcggata tcgtcggcgt gccagacggt gtgtggtata cttacggggt
    15961 ttttcttcat tgtatggtcc gtcatgtcag agcccctcga tctcaatcag ttagcgcaaa
    16021 aaattaaaca gtgggggctg gaactgggct ttcagcaggt aggtattacc gataccgatc
    16081 tcagcgagtc cgagcccaaa ctgcaagcat ggctggacaa acaataccac ggcgaaatgg
    16141 actggatggc acgtcacggt atgctgcgcg ctcgccccca tgagttattg cccggtacgc
    16201 tgcgcgtgat cagcgtgcgg atgaattacc ttcctgctaa cgccgcattt gccagcacgc
    16261 tgaaaaaccc caaactcggc tatgttagcc gttatgcgct gggccgtgac tatcacaaac
    16321 gtctgcgcaa ccgactcaaa aagctgggcg agatgattca gcaacattgt gtttcgctga
    16381 attttagacc gtttgtcgat tctgcgccta ttctcgagcg cccgttagct gaaaaagctg
    16441 ggctcggctg gacaggtaag cactcactta tcctcaatcg cgaggccggt tcgttcttct
    16501 ttttaggcga attgctggtc gatattccgc tgcccgtgga tcaaccagtc gaggaaggat
    16561 gcggcaaatg cgtggcctgt atgacgattt gcccgaccgg tgccatcgtc gagccatata
    16621 ccgtcgatgc tcgccgctgt atctcttatc tcaccatcga actggaaggg gcgatcccgg
    16681 aagagttgcg accgttaatg ggaaaccgta tttacggttg cgatgactgc cagcttatct
    16741 gcccgtggaa tcgctattca caactcacta cagaagacga tttcagcccg cgtaagccgc
    16801 tacacgcacc ggaactcatt gagttattcg cctggagcga agagaagttt ttaaaagtca
    16861 cggaaggttc ggcgattcgc cgtataggtc acctgcgctg gctgcgtaat atcgccgtgg
    16921 cattaggcaa tgcgccctgg gatgaaacga ttctggcggc gctcgagagt cgcaaaggtg
    16981 agcacccact tcttgatgag cacatagcgt gggcgatggc gcagcaaatc gagaggcgaa
    17041 atgcgtgcat agtcgaagtg caattgccga aaaaacagcg tctggttcgg gtaattgaaa
    17101 aagggttacc gcgtgacgcc tgattcatcc acagcttgtg aataaaaata aaaacgcatt
    17161 gcaattcaag acgaataaaa tcgtcaagtg atcgactcga caaaacgaaa tcttaatttt
    17221 taaattatct atcaataggt taaaaaaata ctattcaccg tgcgaagttt ctttgaaatt
    17281 aagattacgg cctgaggctg tggataactc tgtttacaag agtttgtcag agacaacaaa
    17341 aaccagcaat aacgcatcgc gtcgctgtgg atattttatt gagagaaga
//
LOCUS       DBFIFI010000084        17360 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.084, whole genome shotgun sequence.
ACCESSION   DBFIFI010000084 DBFIFI010000000
VERSION     DBFIFI010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.084"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..596)
                     /gene="espY1"
                     /locus_tag="N7B39_002669"
     CDS             complement(<1..596)
                     /gene="espY1"
                     /locus_tag="N7B39_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector EspY1"
                     /protein_id="HGU2422624.1"
                     /translation="MKVSVPGMPATLLNMSNNDIYKMVSGDKMDMKMNIFQRLWETLR
                     HLFWSDKQTEAYKLLFNFVNKKAGNINASKYFTGAVNENEKEKFIHSLELFNELKTCA
                     KNPDEMVAKGNMSWVAQTFGDIELSVTFFIENKEICTQTLQLHKGPGNLGVDLREAYL
                     PGVDMRDCYLGLKTMKGHNKVLYLEPGWNANLDGATLDG"
     gene            complement(891..1706)
                     /gene="djlA"
                     /locus_tag="N7B39_002670"
     CDS             complement(891..1706)
                     /gene="djlA"
                     /locus_tag="N7B39_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HGU2422625.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            1961..4315
                     /gene="lptD"
                     /locus_tag="N7B39_002671"
     CDS             1961..4315
                     /gene="lptD"
                     /locus_tag="N7B39_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HGU2422626.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINANHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVSIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            4368..5654
                     /gene="surA"
                     /locus_tag="N7B39_002672"
     CDS             4368..5654
                     /gene="surA"
                     /locus_tag="N7B39_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HGU2422627.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            5654..6643
                     /gene="pdxA"
                     /locus_tag="N7B39_002673"
     CDS             5654..6643
                     /gene="pdxA"
                     /locus_tag="N7B39_002673"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HGU2422628.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            6640..7461
                     /gene="rsmA"
                     /locus_tag="N7B39_002674"
     CDS             6640..7461
                     /gene="rsmA"
                     /locus_tag="N7B39_002674"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HGU2422629.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPFQES"
     gene            7464..7841
                     /gene="apaG"
                     /locus_tag="N7B39_002675"
     CDS             7464..7841
                     /gene="apaG"
                     /locus_tag="N7B39_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HGU2422630.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            7848..8696
                     /gene="apaH"
                     /locus_tag="N7B39_002676"
     CDS             7848..8696
                     /gene="apaH"
                     /locus_tag="N7B39_002676"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HGU2422631.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     GEAVAS"
     gene            complement(8693..9007)
                     /locus_tag="N7B39_002677"
     CDS             complement(8693..9007)
                     /locus_tag="N7B39_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="HGU2422632.1"
                     /translation="MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFS
                     RIRPPERLNPILLLVDGKEYVLMTHETATVPVNALGTKFCDASAHRTLIKGALDFMLD
                     GI"
     gene            complement(9010..9243)
                     /locus_tag="N7B39_002678"
     CDS             complement(9010..9243)
                     /locus_tag="N7B39_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HGU2422633.1"
                     /translation="MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKH
                     AATRWREENAEAIAALNQLADETGCFSDEYRSF"
     gene            complement(9329..9808)
                     /gene="folA"
                     /locus_tag="N7B39_002679"
     CDS             complement(9329..9808)
                     /gene="folA"
                     /locus_tag="N7B39_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HGU2422634.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTRESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(10000..11862)
                     /gene="kefC"
                     /locus_tag="N7B39_002680"
     CDS             complement(10000..11862)
                     /gene="kefC"
                     /locus_tag="N7B39_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HGU2422635.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYICLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(11855..12385)
                     /gene="kefF"
                     /locus_tag="N7B39_002681"
     CDS             complement(11855..12385)
                     /gene="kefF"
                     /locus_tag="N7B39_002681"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HGU2422636.1"
                     /translation="MILIIYAHPYPHHSHANKRMFEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(12493..13824)
                     /locus_tag="N7B39_002682"
     CDS             complement(12493..13824)
                     /locus_tag="N7B39_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2422637.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYILVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(13882..14169)
                     /gene="fixX"
                     /locus_tag="N7B39_002683"
     CDS             complement(13882..14169)
                     /gene="fixX"
                     /locus_tag="N7B39_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HGU2422638.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(14166..15452)
                     /locus_tag="N7B39_002684"
     CDS             complement(14166..15452)
                     /locus_tag="N7B39_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HGU2422639.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTNVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(15503..16444)
                     /locus_tag="N7B39_002685"
     CDS             complement(15503..16444)
                     /locus_tag="N7B39_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HGU2422640.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINAFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYASVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(16459..17229)
                     /locus_tag="N7B39_002686"
     CDS             complement(16459..17229)
                     /locus_tag="N7B39_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HGU2422641.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
BASE COUNT         4268 a         4816 c         4309 g         3967 t
ORIGIN      
        1 ccgtccaggg tagcaccgtc caggttagca ttccacccag gttccaagta aaggacttta
       61 ttgtgacctt tcattgtttt gagaccaagg taacaatccc tcatgtcaac gccgggaagg
      121 taagcctctc taagatcaac gcccaagtta cctgggcctt tatgcagctg caacgtctga
      181 gtacatatct ccttattttc aataaaaaaa gtgacactta actcgatatc accgaaggtc
      241 tgtgcgaccc agctcatatt gccctttgcg accatctcat ccggattttt tgcgcatgtt
      301 ttgagttcat tgaataattc cagactatgg ataaactttt ctttctcatt ctcgttgaca
      361 gccccagtaa agtattttga ggcgttgatg ttgccagcct ttttattcac gaaattgaac
      421 agaagtttat aagcctcagt ctgtttatct gaccagaaca gatgacgtaa cgtctcccac
      481 aagcgttgaa agatattcat cttcatgtcc atcttgtccc cgctcaccat cttataaata
      541 tcgttattgc tcatatttaa aagtgtagcc ggcatgcctg gaactgatac tttcatggta
      601 atagcctcgc attgagttaa aaactcatga tgcgcgctct gaataaatat actttcaaaa
      661 atcgctcatt cagatatgtg ttttttattt ttaagacgta aaaatataat tccgctcgcc
      721 gtaaagctct caaccttaag cagggtcaac gcgataaaac caacagaaac cctcccgtaa
      781 accgtatatt gagcctgtcc accattctga gtaaacgcct tttctcaaag aagtgaccgc
      841 cagggcggcc accttttatc gtgggcagtt actcagcatc atttacaggg tcatttaaac
      901 cctttctgct gcttaatcag ctcataggcc tgctgaattt cctgcgcttt ctgcttcgcc
      961 atctccatca tctcaggcgg caaacctttc gccaccagct tatcgggatg gtgttcactc
     1021 atcagcttac ggtaggcacg tttgatggtg gtcgcatcat ccgtcggctt cacgcccagc
     1081 acattacagg catcttccag cgttgggcca cgctgcgctt gctgccagtt accaccgcca
     1141 gattgctgct gataaccgcc gccaaactgt gcaccgccct gcatcatgcg caaaaactgg
     1201 tcaaactggg cgcgggagat ccctaattct tcagcaatga catacagcac cgcccgttca
     1261 ttcgggtgca gtgaaccatc agcaaacgcc gcctgaatct ggatctccag aaacatacga
     1321 attaagtcaa aacgaccaaa gcagacactg cgaaactggc gcatcttttc gcgcagcggg
     1381 taattgtctg attttcccac ccggaacgca ttttgcgccg cagtacggga agcgccatga
     1441 agattcattc ggtccatcaa ctggctggcg atatgaatat cagcctccgt gacgcgacct
     1501 ttggatttgg ttaaatgccc catcacttca aaagtggtgg caaaaaacag cgcctgacgc
     1561 tcacgctggt tggcgaacca cgccatttta cggctacggg ctttatcaaa catatggcca
     1621 attaacaggc ctaacactac gccccaaaag ccgccgccca tcagtaaggc cacggccacg
     1681 ccaatgattt ttccccaata ctgcatatat tccccaaatc gacacacgga tatcagggct
     1741 atctcccaca atataaaggt gcttttaccg ttttccggct gcggtcaatt gtgggacatc
     1801 gcctataatt tgcattatca tacctgtcat tcaatgccgt gcctaacacc actgacgcta
     1861 ttcggacagg attaacacta gcgtagagat gacgagtacg ttagtctctg accgtttgtc
     1921 acgcgcaacg ttaccgatga tggaacaata aaatcaacgt atgaaaaaac gtatccccac
     1981 tctcctggcc accatgattg ccaccgccct ttatagtcaa cagggactgg cagccgacct
     2041 cgcctcacag tgcatgttgg gcgtgccaag ctatgaccgt cctctggtac agggcgatac
     2101 caatgactta cccgtgacta tcaatgctaa ccacgcgaaa ggggactacc cggatgacgc
     2161 cgtgtttact ggcagcgtgg atatcatgca gggtaacagc cgtctgcagg ccgacgaagt
     2221 gcagctccat caaaaagagg caccaggaca accggagccg gtacgtaccg ttgatgcgct
     2281 cggtaatgtc cattacgacg ataaccaggt gatcctcaaa gggccgaaag gctgggcgaa
     2341 tctgaacacc aaagatacca acgtctggga aggtgattac cagatggtgg gtcgccaggg
     2401 tcgcggtaaa gcggacctga tgaaacaacg tggcgaaaac cgctatacca ttctggataa
     2461 cggtagcttt acctcctgtc tgccgggttc tgacacctgg agcgtggtag gtagcgaaat
     2521 tattcatgac cgcgaagaac aagttgcgga gatctggaac gcccgcttta aggtgggtcc
     2581 ggtatcgatc ttttatagcc cctatttgca gttgccggtg ggtgacaaac gtcgctctgg
     2641 tttcttgatc ccgaacgcca agtacaccac caccaactac tttgagttct acctgccata
     2701 ttactggaac atcgcgccaa atatggatgc caccatcacg ccgcattata tgcatcgtcg
     2761 tggcaacatc atgtgggaga acgaattccg ctacctctcc caggcgggcg ctggcttgat
     2821 ggaactggac tatctgcctt cagataaagt ctatgaagat gaacacccga acgatgacag
     2881 ttcacgtcgt tggttattct actggaacca ctccggggtc atggatcagg tgtggcgttt
     2941 caacgtcgac tacaccaagg tcagcgatcc tagctacttc aatgatttcg ataacaagta
     3001 cggttccagt actgacggct acgcaacgca aaaattcagc gttggctatg cggtgcaaaa
     3061 cttcaatgcc accgtttcaa ccaagcagtt ccaggttttc agcgaacaga acaccagtag
     3121 ctactcggca gagccgcagt tagacgttaa ttactaccag aatgatgttg gtccgtttga
     3181 tacgcgtatt tacggccagg cagtgcactt tgttaacacc agagacgaca tgcctgaagc
     3241 aacccgtgtt cacctggaac cgaccatcaa tttgccgctc tctaataact ggggcagcat
     3301 caataccgaa gcgaagttgc tggcaaccca ttatcagcaa accaatcttg actggtataa
     3361 ctccagaaac acgaccaagc tggacgaatc cgttaaccgc gtaatgccgc aattcaaagt
     3421 tgacggcaaa atggtctttg aacgcgatat ggaaatgctg gctccgggtt atacccaaac
     3481 gctggaaccg cgcgcgcagt atttgtacgt gccgtatcgc gatcagagcg acatctataa
     3541 ctacgactcg tctctgctgc aatctgacta ctctggcctg ttccgggacc ggacttacgg
     3601 cggtcttgac cgtattgcct ccgctaacca ggtgacgacc ggtgtcacat ctcgcatata
     3661 tgatgatgct gccgttgaac gttttaatat ttccgttggt caaatctact atttcacgga
     3721 gtctcgcact ggcgatgaca acataacatg ggagaatgac gacaaaacgg gttcactggt
     3781 gtgggcaggc gatacttact ggcgtatctc cgagcgttgg ggattgcgtg gcgggattca
     3841 gtacgataca cgtctggata acgtagcgac cagtaactcc agcattgaat accgtcggga
     3901 tgaagaccgt ctggtacagc tgaattaccg ttacgccagc ccggaatata ttcaggctac
     3961 gctgcctaag tactattcca ctgctgagca atataagaat ggtatttcgc aggtaggtgc
     4021 tgtcgccagc tggccaattg ccgatcgttg gtccattgtt ggggcctact actacgacac
     4081 caatgcgaac aagcaagccg actctatgtt aggtgtgcaa tacagctcct gctgctatgc
     4141 aattcgcgtc ggttacgagc ggaagctgaa cggttgggat aacgataaac aacatgcggt
     4201 atatgacaac gcaatcggct ttaacatcga acttcgcggc ctgagctcca actacggtct
     4261 gggtacgcaa gagatgctgc gttcgaacat tctgccgtat caaaacactt tgtgatttgt
     4321 tgatttacca cgtaatccgc agtgcggtta attgaaatgg aaaaagtatg aagaactgga
     4381 aaacgctgct tctcggtatc gccatgatcg cgaataccag tttcgctgcc ccccaggtag
     4441 tcgataaagt cgcagccgtc gtcaataacg gcgtcgtgct ggaaagcgac gttgatggat
     4501 taatgcagtc ggtaaaactg aacgctgctc aggcaaggca gcaacttcct gatgacgcga
     4561 cgctgcgcca ccaaatcatg gaacgtttga tcatggatca aatcatcctg cagatggggc
     4621 agaaaatggg agtgaaaatc tccgatgagc agctggatca ggcgattgct aacatcgcga
     4681 aacagaacaa catgacgctg gatcagatgc gcagccgtct ggcttacgat ggtctgaact
     4741 acaacaccta tcgtaaccag atccgcaaag agatgattat ctctgaagtg cgtaacaacg
     4801 aggtgcgtcg tcgcatcacc atcctgccgc aggaagtcga atccctggcg cagcaggtgg
     4861 gtaaccaaaa cgacgccagc actgagctga acctgagcca catcctgatc ccgctgccgg
     4921 aaaacccgac ctctgatcag gtgaacgaag cggaaagcca ggcgcgcgcc attgtcgatc
     4981 aggcgcgtaa cggcgctgat ttcggtaagc tggcgattgc tcattctgcc gaccagcagg
     5041 cgctgaacgg cggccagatg ggctggggcc gtattcagga gttgcccggg atcttcgccc
     5101 aggcattaag caccgcgaag aaaggcgaca ttgttggccc gattcgttcc ggcgttggct
     5161 tccatatact gaaagttaac gacctgcgcg gcgaaagcaa aaatatctcg gtgaccgaag
     5221 ttcatgctcg ccatattctg ctgaaaccgt cgccgatcat gactgacgaa caggctcgtg
     5281 tgaaactgga acagattgct gctgatatca agagtggtaa aacgactttt gctgccgcag
     5341 cgaaagagtt ctctcaggat ccaggctctg ctaaccaggg cggtgatctc ggctgggcta
     5401 caccagatat tttcgatccg gccttccgtg acgccctgac ccgcctgaac aaaggtcaaa
     5461 tgagtgcacc ggttcactct tcattcggct ggcatttaat cgaactgctg gatacccgta
     5521 atgtcgataa aaccgacgct gcgcagaaag atcgtgcata ccgcatgctg atgaaccgta
     5581 agttctcgga agaagcagca agctggatgc aggaacaacg tgccagcgcc tacgttaaaa
     5641 tcctgagcaa ctaatggtta aaacccaacg tgttgtgatc actcccggcg agcccgccgg
     5701 gattggcccg gacttagttg tccagcttgc acagcgtgag tggccggtcg aactggttgt
     5761 ttgtgccgat gccactctcc ttaccgaccg ggcagcgatg ctcggtttgc cgctcaccct
     5821 ccgcccttat tcccccaact cccctgcaca accgcaaact acgggcacat taacgctact
     5881 tcctgtcgcg ctacgtgaat ctgtcactgc ggggcagtta gcgattgaaa atgggcatta
     5941 cgtggtggag acgctggcgc gagcgtgtga tggctgtctg aacggtgaat ttgctgcgct
     6001 gatcacaggc cccgtgcata aaggcgtcat taacgacgca ggcattccgt ttaccggtca
     6061 taccgagttt ttcgaagagc gttcgcaggc gaaaaaagtg gtgatgatgc tggcgaccga
     6121 agaacttcgc gtggcgctgg caacgacgca tttaccgctg cgcgatatcg cagatgctat
     6181 cacccctgca cttttgcacg aagtgattgc tattttgcat cacgatttgc gtacaaaatt
     6241 tggtattgcc gaaccgcgca ttctggtctg cgggctgaat ccgcacgcgg gcgaaggcgg
     6301 tcatatgggt acggaagaga tagacaccat tattcctgtg ctcgacgagc tgcgggcgca
     6361 ggggatgaaa ctcaacgggc cgctgcctgc cgataccctg tttcagccga aatatctcga
     6421 taacgccgac gccgtgctgg cgatgtacca cgatcagggt cttcccgtgc taaaatacca
     6481 gggcttcggg cgcggtgtga acattacgct gggcctgccc tttattcgca catcagtgga
     6541 ccacggcacc gcacttgaac tggcgggacg tggcgaagcc gatgtcggca gttttattac
     6601 ggcgcttaat ctcgccatca aaatgattgt taacacccaa tgaataatcg agtccaccag
     6661 ggccacttag cccgtaaacg cttcgggcaa aactttctca acgatcagtt cgtgatcgac
     6721 agcattgttt ctgccattaa cccgcaaaag ggccaggcaa tggtcgaaat cggccccggt
     6781 ctggcggcat tgaccgagcc ggtcggcgaa cgtctggacc agctgacggt catcgaactt
     6841 gaccgcgatc tggcggcacg tctgcaaacg catccattct taggcccgaa actgacgatt
     6901 tatcagcagg atgcgatgac ctttaacttt ggcgaactgg ccgagaaaat gggtcagccg
     6961 ctgcgtgttt tcggcaacct gccttataac atctccacgc cgttgatgtt ccatctgttt
     7021 agctatactg atgccattgc cgacatgcac tttatgttgc aaaaagaggt ggtgaatcgt
     7081 ctggttgcag gaccgaacag caaagcgtat ggtcgattaa gcgtcatggc gcaatactat
     7141 tgcaacgtga tcccggtact ggaagttccg ccgtcagcct ttacaccacc acccaaagtg
     7201 gattccgccg tcgtgcgcct ggttcctcat gcaacgatgc ctcacccggt taaagatgtt
     7261 cgtgtgttga gccgcatcac caccgaagcc tttaaccagc gtcgtaaaac cattcgtaac
     7321 agcctcggca acctgtttag cgtcgaggtg ttaacgggaa tggggatcga cccggcgatg
     7381 cgagcggaaa atatctctgt cgcgcaatat tgccagatgg cgaactatct ggcggagaac
     7441 gcgccttttc aggagagtta acgatgatca attcgccccg agtgtgtatt caggttcaaa
     7501 gcgtctacat tgaggcacaa tcttcacctg ataatgaacg ttacgttttt gcttataccg
     7561 taaccatacg caatctgggg cgagcgccag tgcagttgtt ggggcgttac tggctgatca
     7621 ccaatggcaa tggccgtgaa accgaagtcc agggcgaagg agtggttggc gtccagccac
     7681 ttatcgcgcc tggcgaagag taccagtaca ccagcggtgc aatcattgaa accccgctgg
     7741 gcaccatgca gggtcactac gaaatgatcg atgaaaatgg cgtccctttc agcatcgaca
     7801 ttcccgtatt ccgactcgcc gttcccacac tcattcatta aaagaatatg gcgacatacc
     7861 ttattggcga cgttcatggt tgttacgatg aactgatcgc attgctgcat aaagtagaat
     7921 ttacccctgg gaaagatacc ctctggctga cgggcgatct ggtcgcgcgc ggcccgggtt
     7981 cgctggatgt tctgcgctat gtgaaatcct taggcgacag cgtacgtctg gtgctgggca
     8041 atcacgatct gcatctgctg gcagtatttg ccgggatcag ccgcaataaa ccgaaagatc
     8101 gcctgacacc gctgctggaa gcgccggatg ccgacgagct gcttaactgg ctgcgtcgcc
     8161 agcctctgct gcaaatcgac gaagagaaaa agctggtgat ggcccacgca gggatcacgc
     8221 cgcagtggga tctgcagacc gccaaagagt gcgcgcgcga tgtagaagcg gtgctgtcga
     8281 gtgactccta tcccttcttt ctcgacgcta tgtacggcga tatgccaaat aactggtcac
     8341 cggaattgcg ggggctggga agactgcggt ttatcaccaa cgcctttacc cgtatgcgtt
     8401 tttgcttccc gaacggtcaa ctggatatgt acagcaaaga atcgccggaa gaggcccctg
     8461 ccccactgaa accgtggttt gcgattcctg gacctgtcgc tgaagaatac agcatcgcct
     8521 ttggtcactg ggcatcgctg gaaggcaaag gtacgccgga aggtatttac gcgctggata
     8581 ccggctgctg ctggggtggg tcattaacct gcctgcgctg ggaagataaa cagtattttg
     8641 tccagccgtc gaaccggcat aaggatttgg gtgagggaga ggcggtcgcg tcttaaatcc
     8701 cgtcgagcat aaaatctaat gctcctttaa tcaacgttcg gtgcgcgctg gcatcgcaaa
     8761 atttcgttcc cagcgcgtta actggaacag ttgcagtctc atgcgtcatg agcacatatt
     8821 ctttgccgtc taccagtaaa aggattgggt taagccgttc tggtgggcga atacggctaa
     8881 atcgttcaat aggtgttaat ggaataacaa tacggcgatt aatcacacca ataatgtcgc
     8941 tggtaacatc gataacaaag ggaaacgcgg cgttcctgct gcgactgcgg tataccgtaa
     9001 attgcatcgc tagaagctcc ggtactcatc agagaaacat ccggtttcat cagccaattg
     9061 atttaacgca gcgatagctt cagcgttctc ttcacgccaa cgtgttgctg catgtttttt
     9121 aagttcagca ttgagcgcaa ccgtaagggt ggcgctcata tttaagcctg catcacgagc
     9181 gcgattgact aactcacggt cgacggtgac ggtcacactt tgtgtggtac gttttgcagt
     9241 catgatatcg tgctcctgta caatttatac gtctagtata cacggatatc tgcacgtatc
     9301 agtgatgccg gaattctaat atacaaaatt accgccgctc cagaatctca aaacaatagc
     9361 tgtgagagtt ctgcgcatca gcgtcgtgga attcgctgaa taccgattcc cagtcatccg
     9421 gctcgtaatc cggaaaatgg gtgtcgcctt ccacttctgc gtcgatatgc gtcaggtaca
     9481 gtttttgcgc tttcggcagg aactgctcat aaacgcgacc gccgccaatc accatgattt
     9541 ctggtacgtc accacacgcc gcgatggctt catccaccga cttcacccac gttacgcgat
     9601 cgtccgtacc cggttgactg ctgaggataa tatttttgcg tcctggcaac ggacgaccga
     9661 tggattccct ggtatggcgg cccataatta cgggtttatt taaggtgttg cgtttaaacc
     9721 aggcgagatc ggcaggcagg ttccacggca tggcgttttc catgccgata acgcgatcta
     9781 ccgctaacgc cgcaatcaga ctgatcattg agatttcccg ataaaaaaaa ttgtcgccac
     9841 tatacgtaaa gcgtaaaccg tcgtcgactg gtgcgaggat gatgttgagg aaaattttat
     9901 attctgctgg cgagtccacg ctctctccct ggactcgccg cattacaatg aaacaaaaac
     9961 aaacagttag ctgtaaagtg tgatttacgt cactctttat taggatgagg gtttcgtttc
    10021 cggttcatcc gccatgttgc cggtatgttt accttcttct gttccctgcc agccatgtcg
    10081 ttgaattaat gacagatgtt cgcggtcctc ggtgatgatc tcacttaaca tcgcgctggt
    10141 gcgtttatag accgccgcgc gggctttggt gtcattctca accattgcca tctcttccac
    10201 catctgaata ttaaagcggc ggaacacatc ggcacgttct cgcgcttcat acggccccag
    10261 acctaaactt tccagcgcca gacgcccggt tttcagcgca ccttcaaagg tttcacgctc
    10321 tggcttttca acgcctgcct gacgcaaaca aatgtagtga tcgacatcgc gggcgcgggc
    10381 aataatctgc aaatgcggga aatgttcttt caccatctcc gtcagttgca ggttggtttg
    10441 cggatcgtca atggcgttaa tcagcacttc cgctttcgcc gctcccgcag attccagtaa
    10501 atccatccgc gtggcatcgc cataaaacac tttcatacca aatttacgca gcgtttcgat
    10561 atggtccgga tcgtgatcga gcaccaccat tttcaccccg ctggagagca gtaaacgtcc
    10621 ggtgatctgc ccgaaacgac cgaatccggc aatgattacc cgcggctgtt cttcgtcgat
    10681 ctcatcggct tcacgcgcct cttcagtaga agattgctca aggcgattga ggatcaccag
    10741 cagaatcggc gttgccgcca tcgacagcgc caccgccagg gtcagcgatt tcgcccactc
    10801 cggctccagc acattcgcca tctgcgccgc gccaaatacc acaaaggcaa actcactgcc
    10861 ctgccctaac aacaccgcaa accagcgacg ctgtttattt ggcacctgca acggtcgggc
    10921 aatcagccac agcatggcga ttttgatgat gaggaaaccg agcagcaaaa tgacaatgcg
    10981 caatgggttt tcaatcagcg tgccaaagtc tatcgacatg ccaacaccga tgaaaaacag
    11041 ccccaacagc aaacctttaa agggttcgat atcgctttcc agcgcatgac ggtattccga
    11101 gcttgccagc agtacgcccg ccagaaacgc gcccatcgcc atcgacaagc cgacctcttc
    11161 cagcagcaaa ccaaagccaa acacaaggaa taacgccacg gcactaaaca cttcccgcaa
    11221 gccagagcgg gcaacaaaac gcagcgccgg acgcgtgaca tagcgcccca gcagtaccac
    11281 cagcaccagc gcgcccgcca cttttaacgc cgagagagca aatgcgccca tcgtcgtcga
    11341 ggcactgctc gccgccagta gcgggatcat cgccaccagc gggattgccg cgatatcctg
    11401 gaacagcagc accgcaaagg cactgcggcc catttgcgtc accatcagat tgcgttcatt
    11461 catcgcctgc atggcaatcg ccgtagagga gagcgccagc gtcatgccga tcaattccgc
    11521 gacctgccag cgcagcccaa gtaacataca gaacagcccc agcaggccgc cgcaaatcac
    11581 catctgcaat gcgccaccgc caaacactgc cgcacgcagc ttccacagcc tttgtggatc
    11641 gagttcgagg ccgataataa acagcatcag caccacgcca atctcggcaa agtgcagaat
    11701 agattcggca tcggtcacca gtcgcagccc ccacgggcca ataatgcagc cggcgatcag
    11761 gtagccaagt accgatccca gaccaagacg tactgcaatg ggtacaatca gcgccgccga
    11821 accgagataa atcagcgcct gaaccagcgt atggctatcc atgatgggcc tcctgccatt
    11881 ccagcagacg ttgcttatag tgacgcgcct gcccttcgag ggtttcgtcg tcacaaataa
    11941 aggtgcagtg catggcaaac ggtggcagcc agttcagccc gcagtagatt gccgtcgcct
    12001 gtagcggctg cgacagcaca tcaaagcccg gatgcgcacc aatttcaaaa tggctttccc
    12061 cgccgccggt cgtcaccgcc cacagcaaat gtttgccatg cagcgccgtg ccgccgtgac
    12121 cgtaagccca gccgtgcgag aagactttat cgatccaaag tttaaggagc ggaggaatgc
    12181 tgtaccactg catcgggtgc tgccagacga tcagatcggc gcgagacagc gcctcctgct
    12241 cggcggcaat atcgatattg aagtcaggat agagttgata aagagagcga atttcgacgc
    12301 cttccagcgt ccttgcctgt tcaaacatcc gtttattcgc atgggaatga tgcggatacg
    12361 gatgcgcata aattataaga atcataggta gcccgcgtgt ttacgccttt gttttcccac
    12421 aaagtgtagt catatattca cgcggcttat agtcaataat attgattcag aaaagagata
    12481 aatctgaaca aattacccca ttctctgccc gcggatcgtc atattgctgg tctgcgccag
    12541 tgacatccct cgagtctccg gggcaaacgc tacggaaatc aacaagccaa acagcgagat
    12601 acccgccccc attagcatcg tgttactgat accgtaatta ttgataaaga tcggtagtgc
    12661 ccaggtcgaa acaatggtgc caatacgact taaggacata atcacgccca cggcagaggc
    12721 gcggatatcc gtcgggaaga gttcattagg atagagccac tgcaaattac ccggcccgcc
    12781 agagaaaaag gcatacaccg caaaggccat cactaccagc cagatcccca tatccgggat
    12841 cagccccaaa accgccagcg ccagcgtcat catggcaaag ctgccaatca acaatggacg
    12901 ccgtccggca gtgtttagcc acagcatcgg cggaatacag ccgagcataa agaacaggct
    12961 aatcaccacg ttccccagtg ccgcgttttt gccaacaccc aatcccaaca aaccaacgat
    13021 ttgcgggcca aaggtgtaaa tggcgaacat tgggatcacc tggcaggtcc agatggcggc
    13081 aacaaacaga acaaaaggaa aatggcggcg attaaacaga tcgcgaaaac gggtttgctg
    13141 cggctgctct tcatcgaaag ccaccggttc gccaaacagt ttgatcatca tctcttcgca
    13201 ctcttttact cgccctttgc gtaataacca gcggggagat tcaggcagtt cgaatcgacc
    13261 aatcaaaatc aacaaacagg ggatcgccgc gctacccagc atccagcgcc agccgccttc
    13321 cacatcataa agccagtagc cgaccagatc ggcacaggtc gcgccgacat accacatggc
    13381 ggcgataaag ctgatggaaa acgcccgctg acgggtactg gagaactcag tgatcatcga
    13441 agtggcgatg ggatagtctg caccgatgac aatgccgata agtacccgca tcaccaacag
    13501 ttcgacgggg gatgaaacaa acatcgtcgc caccgatatc acgccgatgg cgatgatatc
    13561 aatgaggaac attttgcgcc gtccgacttt atcggaaata taaccaaaca gcgatgtgcc
    13621 aacgaacagc ccggcgagcg ttcccgcgcc cagcaagcca atccagtcag cgtccagttt
    13681 tagcgccggc gtcagttgct ccagcgccac gccaatcatt accagtatat aaccatccag
    13741 aaacggacca ccgcttcccc acagcaaaat gcggcggtga atagaggaaa atttaagatc
    13801 gtcaaagttt ctggacggtt gcatgggtta ttcctgtttt ttgcaatgag agtcagtgcg
    13861 gcgttacgta tcaaaccaac atcagccgta acggaactcc acaccaaagg tgccgcgcgg
    13921 gtattcccac tgttccagcg ccgaccccag ccccagaatg cgacaggtgc cgcactccag
    13981 acatccggcg taatcgaagc gcacactgcc gtcatcctgc ttcttgtaca gacctgcggg
    14041 gcacgctttc accagcagct ccagcgcctg tttatcggca tcggctttca caacaatgtg
    14101 cggatgctct tcatcgacat tgaatttatt gacgcccagt ttgacgtcca cattgacggg
    14161 agaagtcata aaacggtcac tcctttcatg ccatccttga tcagattgat gaagcccact
    14221 ttcttgccgt ggcggaggat tttcttgcgc atcagttccg gcgcactgcc gtcaatggtg
    14281 aacaggtcac gcgccacgcc taccgccagt tccgggtagc cgctaaacat gcgtgggtta
    14341 tcaaggaagg ccggtagttt ctggtacata cgcatatcgc gcagcgggcc actctcaaga
    14401 tgctgacgat attccgcgag tttttgctta ctgaaatcgt cgcttttcat cgctgaaagc
    14461 acggtttttg ctgcagcttc cccggcggca atcgccagat ccataccgcg aatggtaaaa
    14521 ccgaggttca tacacattcc ggcggcatca ccggcaatca atacgccgtc accgaccagc
    14581 tccggcagca tgttgatgcc tgcttccggc actacgtgag cggaatattc caccagcttg
    14641 ccacccgcga tcagcggtgc aacggccgga tgttgtttga aatcttccag catttgcggc
    14701 accgattttt tcgcgtcatg cagatgatgc agaccacaaa ccagccccag cgacagggtg
    14761 ttttcattgg tataaagaaa accgccgccc atcaggccat cggtgggtga tcccgcaaat
    14821 aggcaagccg ccccctgatt accctgcaac tgaaaacggt cttcaataac cgacttcggt
    14881 aactcgatca gttccttcac gccaaccgcc acattcgtcg gtttaacgcg ttttgccatc
    14941 cccagctttt cggcaaggat ggagttcact ccatcagcaa ggatcaccgt tttcgcttca
    15001 atcacatcgc catcggcttc tacaccgacg actttgccat cgcgctgtac gaggttatcg
    15061 acgcggatcc cggtaattaa ctgcgcgccc gcttcttcgg cctgctccat cagccaggca
    15121 tcaaatttac tgcgcaaaac ggagtaagaa cgctgggatg gcgaggtttc gtcaccgttg
    15181 cagtagtcca tagtcatcgc tgacttttcc gtcataaacg cgagtttttc atgggtgatc
    15241 aggcgttcta ccggggcgga ttcggcgaaa ccaggaataa tgtgttccag gctgtgggca
    15301 tagagacgcc cgccggtgac gttcttggca cctgcggaat tgccacgctc gataactaac
    15361 acttgcgccc cttcgcgggc gagcaccagt gcggcaaccg aaccggcaag ccctgcaccg
    15421 acgatgatgg cgtcaaagat atcttcggac ataagagctc cctgtcagcg gcaggaccaa
    15481 aatgcagccc tgccagagtg gatcaacgcg ctaaagctgc ggtcagcgcc ggaaggatct
    15541 tcacggcgtc gccaacaatg ccgtaatccg cgtactggaa gatcggcgca tttttatctt
    15601 tattgatggc gaaaatggtt tgcgacgcgt tagcgccaac catgtgctgg atctgcccgg
    15661 agatccccac agctaggtac agttcaggtt tcagcatcag gttggagata ccgacatagc
    15721 gttcgtgctc catccatttt tcgttttctg ccaccggacg agaacaggcc aactccgcac
    15781 ctatcgcctt gcaaagctgt tctgccagcg caatgttctc tttgctgcca ataccgcgac
    15841 cgacgctgac caccaggcgg gctttgtcga gatcgacgct gttgctctgg cgcgcctggg
    15901 ttgccgtgcg ggtaatcgcc acagccggag cctgccactc cacggtgtgc gtttcgccag
    15961 tgcgtgacgc gtctggctga gccgcatcga acgtgccgct gctgatggtc agtaccgcat
    16021 acggcgtggc aatgcgttct tcgccaatcg ccagaccacc gtaaaccata tgtttcactg
    16081 ttgctttacc gtcctgtacg ctgacggtgc tggcatcgtt agacaccgcc gctttaaggc
    16141 gataacccag ttttgccgcc agtaatttgc cgcgacgggt gtttggcaac agcaccaggc
    16201 cgtctgcacc gtgctggcga atagtgtcag ccatgacact ggcgtaatct tcgatcatcc
    16261 gatcgtccgg tttgccgttt aatttccaga catgattagc gccgagctgg attgcctgtg
    16321 cgccgtcggc atcattgagg acaaaggcat tgatttgatt agctaaagcc tgcgcaccgt
    16381 tcatcagttc cggcagacga gaaggggtat cgctgaatac ccagacttga gaaaacgtgt
    16441 tcatagcatc ccctgtaatt aaatgacttt gcgaagattt tcagcaaatg cggcgatctg
    16501 ttcttcgccg tcgccttcaa tcacgatgcg ctgacgctcg cgctgtttcg gcgcggcaac
    16561 ctgttgttct gaccatgctg cctctgcgtt aaaaccgata tccgccgccg accatacctg
    16621 gactggcttt ttcgccgcgc cgagaatggc tttcatcgaa ggaatttgtg gggagttgat
    16681 atcagtggaa acagcaacaa ccgcaggcag tggaatgctt aaggtttcgg tttcatcttc
    16741 cagttcgcgc tcaacggtga gggtatctgc cgtcagggag ataattttgc tgacgccgtt
    16801 aactgccgga atattgagga tttcgcctac cagcaaacca acctgctggg cataaaggtc
    16861 ggaagaacca tcgccacaga ggatcaaatc aaagcctgct ttctgggcgg ctgcagccag
    16921 tgcgctcgcc gtttgttgcg gcagtgcctg ctcgaactgg tcatcaatca ccacaatcag
    16981 ttcatccgga ccgcgcgata gcacatcttt acgccctttg gcgttggtca gggctttacc
    17041 gcccacactt agggccgtca cctgcgcctc tgctgcctgt tgctttagct ggcaagccgc
    17101 ttcaatagcg ttgagatcgt attggcttat tttggcatcg gctttactga agtctaacga
    17161 accatcagca ttattgaccg caatatcctg ttcatcaggc acgcacttat agcaagtaat
    17221 aatcttcatt gcatctccag aaatcatgaa ggaaacgtcg ttcatatcgg tggtgttaat
    17281 tacagatatc atgcccttta atttggaaat aaaactttaa tcaccaatat tgaaaatgtc
    17341 acacgcagat aaatccaact
//
LOCUS       DBFIFI010000085        17261 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.085, whole genome shotgun sequence.
ACCESSION   DBFIFI010000085 DBFIFI010000000
VERSION     DBFIFI010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.085"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(451..1647)
                     /gene="hemY"
                     /locus_tag="N7B39_002687"
     CDS             complement(451..1647)
                     /gene="hemY"
                     /locus_tag="N7B39_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HGU2422642.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1650..2885)
                     /gene="hemX"
                     /locus_tag="N7B39_002688"
     CDS             complement(1650..2885)
                     /gene="hemX"
                     /locus_tag="N7B39_002688"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HGU2422643.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKVELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQQETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PAPAPAPQADTPAAAPQGE"
     gene            complement(2907..3647)
                     /gene="hemD"
                     /locus_tag="N7B39_002689"
     CDS             complement(2907..3647)
                     /gene="hemD"
                     /locus_tag="N7B39_002689"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HGU2422644.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPNYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3644..4585)
                     /gene="hemC"
                     /locus_tag="N7B39_002690"
     CDS             complement(3644..4585)
                     /gene="hemC"
                     /locus_tag="N7B39_002690"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HGU2422645.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4972..7518
                     /gene="cyaA"
                     /locus_tag="N7B39_002691"
     CDS             4972..7518
                     /gene="cyaA"
                     /locus_tag="N7B39_002691"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HGU2422646.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            complement(7558..7878)
                     /gene="cyaY"
                     /locus_tag="N7B39_002692"
     CDS             complement(7558..7878)
                     /gene="cyaY"
                     /locus_tag="N7B39_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HGU2422647.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFRDLLEQAATQQAGET
                     VSFR"
     gene            complement(8040..8182)
                     /locus_tag="N7B39_002693"
                     /pseudo
     CDS             complement(8040..8182)
                     /locus_tag="N7B39_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            8341..8544
                     /gene="yifL"
                     /locus_tag="N7B39_002694"
     CDS             8341..8544
                     /gene="yifL"
                     /locus_tag="N7B39_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGU2422648.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            8581..9405
                     /gene="dapF"
                     /locus_tag="N7B39_002695"
     CDS             8581..9405
                     /gene="dapF"
                     /locus_tag="N7B39_002695"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HGU2422649.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            9402..10109
                     /gene="yigA"
                     /locus_tag="N7B39_002696"
     CDS             9402..10109
                     /gene="yigA"
                     /locus_tag="N7B39_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HGU2422650.1"
                     /translation="MKQPGEELQETLTELDDRVVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQALANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            10106..11002
                     /gene="xerC"
                     /locus_tag="N7B39_002697"
     CDS             10106..11002
                     /gene="xerC"
                     /locus_tag="N7B39_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HGU2422651.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQNWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKTPRHLPKNIDVDDINRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNALSWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            11002..11718
                     /gene="yigB"
                     /locus_tag="N7B39_002698"
     CDS             11002..11718
                     /gene="yigB"
                     /locus_tag="N7B39_002698"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HGU2422652.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEQEALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            11802..13964
                     /gene="uvrD"
                     /locus_tag="N7B39_002699"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             11802..13964
                     /gene="uvrD"
                     /locus_tag="N7B39_002699"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HGU2422653.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(14009..14911)
                     /locus_tag="N7B39_002700"
     CDS             complement(14009..14911)
                     /locus_tag="N7B39_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HGU2422654.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSIV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVREIDEKANRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSRQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCDK"
     gene            complement(14994..15758)
                     /gene="yigE"
                     /locus_tag="N7B39_002701"
     CDS             complement(14994..15758)
                     /gene="yigE"
                     /locus_tag="N7B39_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HGU2422655.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     MLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            15920..15976
                     /gene="ysgD"
                     /locus_tag="N7B39_002702"
     CDS             15920..15976
                     /gene="ysgD"
                     /locus_tag="N7B39_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HGU2422656.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            16128..17078
                     /gene="corA"
                     /locus_tag="N7B39_002703"
     CDS             16128..17078
                     /gene="corA"
                     /locus_tag="N7B39_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HGU2422657.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
BASE COUNT         3943 a         4508 c         4611 g         4199 t
ORIGIN      
        1 tagttttaac ggcaattaat atatgtgtta ttaattgaat gaattttatc attcataata
       61 agtatgtgta ggatcaagct caggttaaat attcactcag gaagttatta ctcaggaagc
      121 aaagaggatt acagaattat ctcataacaa gtgttaaggg atgttatttc ccgattctct
      181 gtggcataat aaacgagtag atgctcattc catctcttat gttcgcctta gtgcctcata
      241 aactccggaa tgacgcagag ccgtttacgg tgcttatcgt ccactgacag atgtcgctta
      301 tgcctcatca gacaccatgg acacaacgtt gagtgaagca cccacttgtt gtcatacaga
      361 cctgttttaa cgcctgctcc gtaataagag caggcgtttt tttatgtgtc aggaaggtcc
      421 cggaggtgct tgcctccggg tgagaaggaa ctactgtggt gggttgttct gcaacgttaa
      481 catcaaaccg tcgcgacgca tcgctgcagc ttcttccggc ttgtgcagtc tgtccagcgc
      541 gtcggcaagc catgcgtaat cgtaggcgtc cggacgttgt ttcagcgctg cgcggaaggc
      601 gagcgatgct tcctgccatt ctccgtgctt catcagtgac tggcccagtg tgctccacaa
      661 cagcgggcga tcgccgacgt ttttgatttg ctggcgcagc actttttcca gctgttccgg
      721 attgtttgtt ttcagtcgag gaatcggcag cagtaggcga tcgtcgtact ggcgtttcag
      781 gccatcgatg ataatttgct gggcagtatc atgatcgtca cattcaataa gatgttccgc
      841 cattgccacc tgcaacgcta cctgatgacg cgttttccgg ctttggtttt tccaccagtt
      901 acgcaaacct tcgctaccgt tatcggcacg cgcctgatcc atcaggccaa tccatgcctg
      961 ttgttccagc attgcacgat gttcttcatc accaacatgg gctttcgcca ttgatgggat
     1021 aatatccagc agcgaactcc atgcacctgt gcggatatac gcctgttccg ccagacgtaa
     1081 tacttccgga tggcgtggcg taacttccag cagcttatcc acgccgtggc gtgcagcatg
     1141 gttttcatta cgggccagtt gcagacgtac acgggtgatt tctaccggaa tggtgtcatt
     1201 gccggccagc tccgctgcgc gttccagatg ttggttggcg cgcgcttcat caccacgttg
     1261 ttgcgcggct tcggcagcca gtagatagtt caccaccggt tgttcagcgt gatcggcatt
     1321 tttcgccatc agcttttcaa cttgctgata atcgccttcc gccagtttca gcagcgcctg
     1381 ttcggtctgc ttacgtgcac ggcgacgctt acgcccgaca aaccacccac gggtgtgcgc
     1441 gccagtgcgg aagatccgcc gcagtagcca ctcaatggca aacagcacta ccatcgccag
     1501 aatcaatatg atcgccaggc ccgtgacgct ggtttcgata ttgtagttgt cggtctggat
     1561 cagcacataa ccctgatgac cggcaatcat cgggccaacc acgatccccg caatcagcaa
     1621 cacaaagagc aataacactt ttagcatggt tattctcctt gcggcgcggc tgccggagta
     1681 tcagcttgcg gtgcaggtgc aggtgcaggt gcaggtgcag gtgcaggtgc aggtgcaggt
     1741 gcaggtgcag gtgcaggttt agcttccgtt gtccccgctg ccggttgtgc cagcaggtta
     1801 cgcacgcgag tctgcatcag tttttccagc atcgcctggc tttgcagggt ttccggaaga
     1861 tccatcgaga tattttgctg gcttaactgg tctacctcgt cgaggaacgc tttggtggtg
     1921 gcatcatcag tatcgtagta agcacgtacc caggtggaga cgttctccag cgcctggcga
     1981 taagtctctt gctggtgacg cggtacagct tgtgctgcga ccagcaggcg agagcgaata
     2041 ttttcgcgca gatagatatc ctgatttggc gctaacagcg gtacggcggt gtcatcacga
     2101 cggcgaatcg taatgaagtt gtccataaag ttctgccagc ttttttgcag attgatacgc
     2161 cattcgctga tggaactgga aagctcttca ccgtcggaat ccatcggcga accatcgctg
     2221 tcattatcgg ccagacgcag gttatctacc tgatttgaaa gctgattaag cttaaggatg
     2281 atgccgtcat aatccacctg cgatactgca gaaaggctgg cgatatcatc ggtaattgcc
     2341 cgacgaacgg taatcagact cgggtcattc atatccgcca ggctggcgtc tgcacttttc
     2401 agcaacgctg cagcggtcgt gacgtcctga tcgctccaca gcttccgtcc ggcgagtttc
     2461 accagaaaat cggcctgagc cagcagccag gttttagcat cgctgccgga aatggtggcg
     2521 accttttgtt ggacttcatc caactgtttt gccagcgttt cttgctgacg attcgcctgc
     2581 ttaagctgtg cagcttgttg cttaataatg ccttccagct cgactttttg gctctcctgg
     2641 gctttttgca atgccgtcag ttggttagcc agggcatcgc tggtggcggt ctgattgacg
     2701 gcctgttgtt taccccagcc atacaaaccg acgcccgccg ccagagcaat agcgatagcc
     2761 accgcgctga gaatcaatgc ggtattgttc ttactctttt tttctgttgc gacaggttgt
     2821 gacgtggtgt ccacggcctc cctggtctct tcaaccacgg cggaggtttt ttcttgttcc
     2881 gtcattatgg cttcctgtta tgagagttat tgtaatgccc gtaaaagcgc atcgttgtca
     2941 gcgttatcgg cgaccttaat gtcttgccag cccagttccc gggcgagttt cgccaaacgc
     3001 tcactgacga ccaatagtcg acagtgtagt aaccagtgct cacgatacca ttgtgggatc
     3061 agcgaccaga gttgctgcaa catttcaccg ctggtaacaa cgaccgtcgt cacctcgcgg
     3121 gattgccagc gcatcgcttc ttctgcacca tcgtaatgga ttgcgcatcg ttgataacat
     3181 tcacaaaaag tgacctcagc accgcgcgcc gtcagggtat ccccaattag ctcacgaccg
     3241 ccattgccac gtaatatcag cgcacgtttg cccgcaatat tttgtaattc aggtaattgt
     3301 agcaagactt cgctgatttc ccgatcctgc gggtagagaa tcttctgtcc gcttacggta
     3361 tgtagtgcca gtgcggtggt gcgtccaatg gcgaaataat taggtagtcg gggccattta
     3421 cgatcttgct gatgtagctg tgattgggca aaagcaaccg cgtgttgcga gagggcaaac
     3481 aacagatcgc tctcccccag cgctgccagt tgatcagcaa gttgcggtaa ttgtcgaccc
     3541 ggagaaaact caatcagcgg aaaatgccag gccacctgcc ccagtgtgcg cagacggctc
     3601 actaactctt ctccagcggg agacgggcgg gtgacaagga tactcatgcc ggggcgtctc
     3661 cgttatagac ttcagcgagg atctcgcgcg cgccgttatt cagtagctct tctgccagcg
     3721 aaatccccat ttgttcggca tcttgcggcg caccgcggcg ttcaccgcga ataatctgcg
     3781 aaccgtccgg cgcgccgacc agcgcacgca gccagatttc gccatcaata agctcggcgt
     3841 agctaccaat tggcacctga catccacctt cgagacgggt attcatggcg cgttctgcgg
     3901 taacgcgcag tgcagtttcg tggtgattca gcgcggcaag cagctcgcga gtgcgtgtat
     3961 catcaaggcg gcattcaata ccgactgcac cttgtcctac cgccggaaga gaaatctcgg
     4021 gtggcaacgc ggcgcgaatg cgggactcca gacctaaacg ttttagtccg gctacggcaa
     4081 gaatgatggc atcgtattcg ccgttatcca gtttgctcag gcgagtgccg acgttgccgc
     4141 gcagggagcg gataatcaga tccgggcggc gttcagccag ttggcactgg cgacgtaaac
     4201 tggacgtccc gacgatactg cctgccggta acgcatccag actgtcatag ttattggaca
     4261 caaaggcatc gcgaggatct tcacgctcac aaatagtgac caggcccaga ccttgcggga
     4321 attcaaccgg cacatctttc attgaatgta cggcgatatc ggcgcgattt tcgaggagcg
     4381 cgacttccag ctcttttaca aataagcctt ttccgccgac tttcgccagc ggcgtatcaa
     4441 gaatcacatc gccgcgcgtc accatcggta ccagttcaac gaccaggccc ggatggctcg
     4501 ccatcaactt gtctttgaca tagtgtgcct gccagagtgc aagtgggctt tggcgtgtgg
     4561 caattcttaa aacattgtct aacatgcttg ttaccgtcat tatcatccgt ggtccatcct
     4621 aacatccttg ccagagtgat gtcagtgttg tggtgaaacg tagacgcctg cgcaaaccgt
     4681 aaaatgaggt ctggcagtgg atcctgacag gcgtttcacg ccgtcgtaat aaggaattta
     4741 cagagaataa acggtgctac acttgtatgt agcgcatctt tctttacggt caatcagcaa
     4801 ggtgttaaat tgatcacgtt ttagaccatt ttttcgtcgt gaaactaaaa aaaccaggcg
     4861 cgaaaagtgg taacggttac ctttgacata cgaaatatcc cgaatgccgc gtgttaccgt
     4921 tgatgttggc ggaatcacag tcatgacggg tagcaaatca ggcgatacgt cttgtacctc
     4981 tatattgaga ctctgaaaca gagactggat gccataaatc aattgcgtgt ggatcgcgcg
     5041 cttgctgcta tggggcctgc attccaacag gtctacagtc tactgccgac attgttgcac
     5101 tatcaccatc cgctaatgcc gggttacctt gatggtaacg ttcccaaagg catttgcctt
     5161 tacacgcctg atgaaactca acgccactac ctgaacgagc ttgaactgta tcgtggaatg
     5221 tcagtacagg acccgccgaa aggtgagctt ccaattactg gtgtatacac catgggcagc
     5281 acctcgtccg tagggcaaag ttgttcctct gacctggata tctgggtctg tcatcaatcc
     5341 tggctcgata gcgaagagcg ccagttgcta caacgtaaat gcagcctgct ggaaagctgg
     5401 gccgcctcgc tgggtgtgga agttagcttc ttcctgattg atgaaaaccg ttttcgccac
     5461 aacgagagtg gcagtctggg gggcgaagat tgtggctcca cccagcatat actgctgctt
     5521 gatgaatttt atcgtaccgc cgtgcgtctc gccggtaagc gtattctgtg gaatatggtg
     5581 ccgtgcgacg aagaagagca ttacgatgac tacgtaatga cgctctacgc acaaggcgtg
     5641 ctgacgccaa atgaatggct ggatctcggc ggcttaagct cgctttccgc tgaagaatac
     5701 ttcggtgcca gcctttggca actctacaag agtatcgatt ctccatacaa agcggtgctg
     5761 aagacactgc tgctggaagc ctattcctgg gaatacccga acccacgcct gctggcgaaa
     5821 gatatcaaac agcgtttgca cgacggcgag attgtatcgt ttggcctcga tccttactgc
     5881 atgatgctgg agcgtgttac tgaatacctg acggcgattg aagatttcac ccgtctggat
     5941 ttagtacgtc gctgcttcta tttaaaagtg tgcgaaaagc tcagccgtga acgcgcctgc
     6001 gtaggctggc gtcgcgcagt gttgagccag ttagtgagcg agtgggggtg ggacgaagct
     6061 cgtctggcaa tgctcgataa ccgcgctaac tggaagattg atcaggtgcg tgaggcgcac
     6121 aacgagttgc tcgacgcgat gatgcagagc taccgtaatc tgatccgctt tgcgcgtcgc
     6181 aataacctta gcgtctccgc cagtccgcag gatatcggcg tgctgacgcg taagctgtat
     6241 gccgcgtttg aagcattacc aggtaaagtg acgctggtaa acccgcagat ttcacccgat
     6301 ctctcggaac cgaatctgac ctttatttat gtgccgccgg gccgggctaa ccgttcaggt
     6361 tggtatctgt ataaccgcgc gccaaatatt gagtcgatca tcagccatca gccgctggaa
     6421 tataaccgtt acctgaataa actggtggcg tgggcatggt ttaacggcct gctgacctcg
     6481 cgcacccgct tgtatattaa aggtaacggc attgtcgatt tgcctaagtt gcaggagatg
     6541 gtcgccgacg tgtcgcacca tttcccgttg cgcttacctg caccgacacc gaaggcgctc
     6601 tacagcccgt gtgagatccg ccatctggcg attatcgtta acctggaata tgacccgaca
     6661 gctgcgttcc gcaatcaggt ggtgcatttt gatttccgta agctggatgt tttcagcttt
     6721 ggcgagaatc aaaattgcct ggtaggtagc gttgacctgc tgtatcgcaa ctcgtggaat
     6781 gaagtgcgta cgctgcactt caacggcgag caatcgatga tcgaagccct gaaaactatt
     6841 ctcggcaaaa tgcatcagga cgccgcaccg ccagatagcg tggaagtctt ctgttatagc
     6901 cagcatctgc gcggcttaat tcgtactcgc gtgcagcaac tggtttctga gtgtattgaa
     6961 ttgcgtcttt ccagcacccg ccaggaaacc gggcgtttca aggcgctgcg cgtttctggt
     7021 caaacctggg gcttgttctt cgaacgcctg aatgtatcgg tacagaaact ggagaacgcc
     7081 atcgagtttt atggcgctat ttcgcataac aaactgcacg gcctgtcagt gcaggttgaa
     7141 accaatcacg tcaaattacc ggcggtggtg gacggctttg ccagcgaagg gatcatccag
     7201 ttctttttcg aagaaacgca agatgagaat ggctttaata tctacattct cgacgaaagc
     7261 aatcgggttg aggtgtatca ccactgcgaa ggcagcaaag aggagctggt acgtgacgtc
     7321 agtcgcttct actcgtcatc gcatgaccgt tttacctacg gctcaagctt catcaacttc
     7381 aacctgccgc agttctatca gattgtgaag gttgatggtc gtgaacaggt gattccgttc
     7441 cgcacaaaat ctatcggtaa catgccgcct gccaatcagg atcacgatac gccgctatta
     7501 cagcagtatt tttcgtgatg aacgtgccgg aaagcgaggc ttatccggca tgcaatctta
     7561 gcggaaactg actgtttcac ccgcctgctg cgtcgccgcc tgttccagca aatcccggaa
     7621 ggtttcgccg ctgcgatcgc aaatccactc atcgcctttc agatcaaaat ggtagccgcc
     7681 ttgtttggtt gccagccata cctggtgcag cggttcctgg cggttgataa tgattttgct
     7741 gccattctca aaggtaatgg tcagcacgcc gccgttgatt tcgcagtcga tatcgctgtc
     7801 gccatcccag tcgtccagac gctcttcaat ggtcagccac agttgatcag ccaggcgatg
     7861 aaattcactg tcgttcattg ttgtatcctg tttttaagtg atggcggcag tatagcggca
     7921 tggggtcagg gcttcaaagt ttgcacctct gcggctgcgt tacggcatga ttcatccgtc
     7981 accggaataa tgatgtctct gtgtagcgaa agatttgtct cttcattagg gcgcagttac
     8041 accacgcctt tccctgtttc tggtaaacat tatgatcagg tttaccgagc gagcatcctc
     8101 acgctgacgg aactaaaaaa gccaacaaac aaaccacatt gcgatagtgc ataaagccat
     8161 cctggcgtga ggtgttgatc acgaaactgc cagcagaaca taaatcctca cgagtaagcg
     8221 ttatactcgc agcatttcct cacttttcag acttcataaa gagtcgctaa acgcttgctt
     8281 ttacgccttc tcctgcgatg atagaaagca gaaagcgatg aactttacag gcaatccata
     8341 atgaaaaacg tgtttaaggc actcactgta ttacttactc tcttcagcct gacgggctgc
     8401 ggtctgaaag gtccgctcta tttcccgcct gcagataaaa atgcaccgcc gccgaccaaa
     8461 ccggtagaga cgcaaacgca atccacggtg ccggataaaa acgatcgcgc cactggcgat
     8521 ggtccatccc aggtgaatta ctaaaagtca gtttctgtac ccgcgtgatt ggagtaaatg
     8581 atgcagttct cgaaaatgca tggccttggc aacgatttta tggtcgtcga cgcggtaacg
     8641 cagaatgtct ttttttcacc ggagctgatt cgtcgcctag ctgatcggca cctgggggta
     8701 gggtttgacc aactgctggt ggttgagccg ccgtatgatc ctgaactgga ttttcactat
     8761 cgcattttca atgctgatgg cagtgaagtg gcgcagtgcg gcaacggcgc acgctgcttt
     8821 gcccgttttg tgcgtctgaa agggctgacc aataagcgcg atatccgcgt cagcaccgcc
     8881 aacgggcgga tggttcttac cgtcaccgat gatgatctgg tccgcgtaaa tatgggcgaa
     8941 cccaacttcg aaccttctgc cgtgccgttt cgcgctaaca aagcggaaaa gacctatatt
     9001 atgcgcgccg ccgagcagac aatcttatgc ggcgtggtgt cgatgggaaa tccacattgc
     9061 gtgattcagg tcgatgatgt cgatactgcg gcggtagaaa cactggggcc tgttctggaa
     9121 agccacgagc gttttccgga gcgcgccaat atcggtttta tgcaagtggt taagcgcgag
     9181 catattcgtt tacgcgttta tgagcgtggg gcaggagaaa cccaggcctg cggcagcggc
     9241 gcatgtgcgg cggttgcagt agggattcag caaggtttgc tggccgaaga agtacgcgtg
     9301 gaactcccag gcggtcgtct tgatatcgcc tggaaaggtc cgggtcaccc gttatatatg
     9361 actggcccgg cggtacatgt ctacgacgga tttattcatc tatgaagcaa ccaggggaag
     9421 aactgcagga aacactcacg gagcttgatg accgggtggt tgtcgattat ctgattaaaa
     9481 atcctgagtt ttttatccgc aatgcgcgcg cagtagaagc gatacgtgtg ccgcatccgg
     9541 tacgcggtac cgtttcgttg gtcgagtggc acatggcccg cgcacgtaat catattcatg
     9601 ttctggaaga gaacatggcg ctgttgatgg aacaggctct cgccaacgaa ggcctgtttt
     9661 atcgcttact ctacctgcag cgcagtctca ccgccgccag cagtcttgat gatatgctga
     9721 tgcgttttca ccgctgggcg cgtgatctcg gcctggcagg tgcgagtctg cgcctgtttc
     9781 cggatcgctg gcgcttaggt gcgccgtcga accacactca tctggcatta agccgtcagt
     9841 ctttcgaacc gctgcgtatt cagcgtctgg ggcaggagca gcattatctg gggccgctta
     9901 acggaccgga gctgctggtg gtactaccgg aagcgaaagc ggtgggatcg gtggcgatgt
     9961 cgatgctggg aagcgatgct gatttaggcg tggtgctgtt caccagccgc gatgccagtc
    10021 actatcaaca agggcaggga acgcagttac ttcatgaaat tgcgctgatg ttgccggagc
    10081 ttctggagcg ttggattgaa cgcgtatgac cgatttacac accgatgtag aacgctacct
    10141 acgttatctg agcgtggagc ggcagcttag tccgataact ctgcttaact accagcgaca
    10201 gcttgaggcg atcatcaatt ttgccagcga aaacggcctg caaaactggc agcaatgcga
    10261 tgcagcgatg gtgcgcaatt ttgctgtacg cagtcgccgt aaagggctgg gcgcggcaag
    10321 cctcgcatta cgcctctctg cgctacgtag cttttttgac tggctggtca gccagaacga
    10381 actcaaagct aacccggcga aaggtgtttc ggcaccgaaa acgccacgtc atctgccgaa
    10441 aaatatcgac gtcgatgata taaatcgcct gttggatatt gatatcaacg atcccctcgc
    10501 tgtgcgcgac cgtgcaatgc tggaagtgat gtacggcgcg ggcctgcgtc tttccgagct
    10561 ggtggggctg gacatcaaac acctcgacct ggagtccggc gaagtgtggg tgatggggaa
    10621 aggcagcaaa gagcgccgcc tgccgattgg tcgtaacgct ttgtcatgga ttgagcactg
    10681 gcttgatttg cgcgacctgt ttggtagcga agacgacgcg ctttttctgt cgaaactggg
    10741 caagcgtatc tccgcgcgta atgtgcagaa acgctttgcc gaatggggca taaaacaagg
    10801 gctgaataat cacgttcacc cgcataaatt acgtcactcg ttcgctacgc atatgctgga
    10861 gtcgagcggc gatcttcgtg gtgtgcagga gctgctgggt catgccaacc tctccaccac
    10921 gcaaatctat actcatcttg attttcaaca ccttgcctcg gtgtacgatg cggcgcatcc
    10981 acgcgccaaa cgggggaaat aatgcgtttt taccggcctt tggggcgcat ctcggcgctc
    11041 acctttgacc tggatgatac cctttacgat aaccgtccgg tgattttgcg caccgagcag
    11101 gaggcgctta cctttgtgca aaattaccat ccggcgctgc gcagcttcca gaatgaagat
    11161 ctgcaacgcc tgcgccaggc ggtacgggaa gcggaacccg agatttatca cgacgtgacg
    11221 cgctggcgtt ttcgctcaat agaacaagcg atgcttgacg ccggattgag cgcggaagaa
    11281 gccagtgcag gcgcacacgc ggcaatgatc aactttgcta aatggcgcag caggatcgac
    11341 gtcccgcagc aaacccacga caccttaaaa cagctggcga agaaatggcc gctggtggcg
    11401 atcaccaacg gtaacgctca gccggaactg tttggtttgg gggattattt tgagtttgtg
    11461 ctgcgcgctg gcccgcacgg gcgctcgaaa ccgttcagcg atatgtactt cctggcggcg
    11521 gaaaaactca acgtgccgat cggcgagatc ttacatgtag gtgacgatct caccactgac
    11581 gtgggtgggg caattcgcag cggaatgcag gcctgttgga tcagaccgga aaatggcgat
    11641 ctgatgcaaa cctgggatag ccgtttactc ccgcatctgg aaatttcccg gttggcatct
    11701 ctgacctcgc tgatataatc agcaaatctg tatatatacc cagctttttg gcggagggcg
    11761 ttgcgcttct ccgcccaacc tatttttacg cggcggtgcc aatggacgtt tcttacctgc
    11821 tcgacagcct taatgacaaa cagcgcgaag cggtggccgc gccgcgcagc aaccttctgg
    11881 tgctggcggg cgcgggcagt ggtaagacgc gcgtactggt gcatcgtatc gcctggctga
    11941 tgagcgtgga aaactgctcg ccatactcga ttatggctgt gacgtttacc aacaaagcgg
    12001 cggcggagat gcgtcatcgt atcgggcaac tgatgggcac cagccagggc ggcatgtggg
    12061 tcggcacctt ccacgggctg gcgcaccgtt tgctgcgtgc gcaccatatg gacgccaatc
    12121 tgccgcagga tttccagatc ctcgatagcg aagaccagct acgcctgctt aagcgtctga
    12181 tcaaagccat gaacctcgac gagaagcagt ggccgccgcg ccaggcaatg tggtacatca
    12241 acagccagaa agatgaaggc ctgcgtccgc atcatattca aagctacggt aatccggtgg
    12301 agcagacctg gcagaaggtg tatcaggcgt atcaggaagc gtgcgatcgt gcaggcctgg
    12361 tggacttcgc cgagctgctg ctgcgcgctc acgagttgtg gcttaacaag ccgcatatcc
    12421 tgcaacacta ccgcgaacgt tttaccaaca tcctggtgga cgaattccag gataccaaca
    12481 acattcagta cgcgtggatc cgcctgctgg cgggcgacac cggcaaagtg atgatcgtcg
    12541 gtgatgacga ccagtcaatc tacggctggc gcggggcgca ggtggagaat attcagcgtt
    12601 tcctcaatga tttccccggt gccgaaacta tccgtctgga gcagaactat cgttctacca
    12661 gtaacattct gagcgccgcg aacgccctga ttgaaaacaa caacgggcgt ctgggtaaaa
    12721 aactgtggac cgatggcgcg gacggtgagc ctatttccct ctattgcgct tttaacgaac
    12781 tcgatgaagc gcgttttgtc gttaaccgca tcaaaacctg gcaggacaac ggtggggcgc
    12841 tcgccgagtg cgccattctc taccgcagca acgcccagtc gcgggtgctc gaagaggcgt
    12901 tattgcaggc cagtatgccg taccgtattt acggcgggat gcgcttcttc gaacgccagg
    12961 aaatcaaaga tgcgctctcg tatctgcgcc tgattgccaa ccgcaacgac gacgcggcct
    13021 ttgagcgtgt ggtgaatacg ccaacgcggg gtattggtga ccggacgctg gacgtggtac
    13081 gtcagacatc gcgcgatcgc cagttaacac tctggcaggc atgtcgtgaa ctgttgcagg
    13141 aaaaagccct cgccggacgt gctgccagcg ccttacagcg gtttatggaa ctgatcgacg
    13201 ccttagcgca ggaaactgcc gatatgccgc tgcatgtaca gactgaccgg gtaattaaag
    13261 actccggcct gcgtaccatg tacgagcagg agaagggcga aaaaggtcag acgcgtatcg
    13321 aaaacttaga ggaactagtg acggcaacgc gccaattcag ctacaacgaa gaagacgaag
    13381 atttaatgcc gctgcaggcg ttcctctccc atgcggcgct ggaagcgggc gaggggcagg
    13441 cggatacctg gcaggatgca gtgcagttga tgacgctgca ctcggcgaaa ggcctggagt
    13501 tcccgcaggt gtttatcgtc ggtatggaag agggcatgtt cccaagccag atgtcgctgg
    13561 atgaaggcgg acgtctggaa gaagaacgcc gtctggccta cgttggtgta acccgcgcga
    13621 tgcagaaact gacgctgacc tacgcggaaa ctcgccgtct gtatggcaaa gaggtttacc
    13681 atcgcccgtc gcgctttatc ggtgagttgc cggaagagtg tgtggaagag gtgcgcctgc
    13741 gcgccacggt aagccgcccg gtcagccatc agcgtatggg tacgccgatg gtcgagaacg
    13801 acagcggcta caagctcggc cagcgtgtac gccacgctaa gtttggtgaa ggcactatcg
    13861 tcaatatgga aggcagcggc gagcatagcc gtttgcaggt ggcattccag ggccagggta
    13921 ttaaatggct ggtggcggca tacgcccggc tggagacggt ataacgttgc gggatgcggt
    13981 gcgccacacc gcatccggca tcagcacctt acttatcaca tttcttcaca tcaatattcc
    14041 agtcagcacc agacgcaacc tgatgtttct gcgcgaaatt atccatattc agcgccacgg
    14101 aaacattcaa taagctgttc agatacacca tcggctgtcc ttccggcaca tcgccgaagc
    14161 ttgcgacata cggcattttc cctgcgtatt tttgccggga accttcggaa atggtcacac
    14221 acaacgtgtc gttgagtttg atccccgcct gattcagcaa ctcgtcgctg atattgctcc
    14281 agacgttgcc gtactgaata tcgagaatcg ggatattgcc cttcacttca ccttttgttg
    14341 cttttgcttt ctgataagaa agttcgacaa cctttgcagg aagctcaggc cccacctgct
    14401 cgaacgtgat cgccccggaa gccagacgtg caccggtgta ggcatacacg tcacgaccgt
    14461 ggaaggtata tgacttttca gaacctttca ggcggttagc tttttcgtcg atttcccgga
    14521 cgctctcaat acccagcgat tccgccacca aagtgagcgt accgttatcc ggtgaaacga
    14581 agtattgacc gtttttggtt ttcagcacta tcgatttacg gtcagtgcct acgcccggat
    14641 cgaccacact gacaaatacc gaaccttgcg gccagtaact ggcggtctga tacaggcggt
    14701 aagcgccttc ccagatgtta tacggtggaa tttcgtgtgt taaatcaaat attttaagat
    14761 tatggtcaac accgaatgcg acccctttca tcgccgagac ggcaccatct ttcaggctaa
    14821 aatcagtttg taaaatcaaa gcgttatccg cataagcgga tgatgatccc agacacgtta
    14881 ataaaaataa cgttgcgagc gtttttttca tttttgtttc ccttcattta tctgatgatt
    14941 ttccagctgt gtagccagcc ccagtttgtc tggcctcctc gagtgtacaa tgcttaacct
    15001 ttccgctcga cggaaatcat ggtgacaaaa ggatagcgtt gccacgggat tgccccgcct
    15061 ttcatataca tatgtgaaat cgtgccatca agataaagca gctgctcaac gttcagtttc
    15121 gctttggcat aacaggcaaa atcataaaaa tttgtcgcct gctggctcaa caaaaacacg
    15181 gcattcccat gtttattaat tccaacaccg ttacgaattt tacgtgaggc gacgttggga
    15241 tgaatacgcg gattaattac actgttttcc atcaacattg gccctgactg caccgcaaac
    15301 tgaatctctt tactggtttt gaaggcatcc agacgaacga tgccgacttt atctcccgcg
    15361 acataaaaca cgccgccagg acggataaag aaattccctt cacctgaagc gagatttaac
    15421 gccaccttct gctgaccgtt ttcgatgtac aaaccgagcg gcgcatagct ttcatcatag
    15481 atgccgccgt tcatcgccat ctgcacctga ccctgactat tcatatccgc cagcagagca
    15541 tgtaaagttc cccacgcttc gccattggct ttttgccagt acattttcac ccgctctgtt
    15601 tgaggattaa cggtatacgc ctgtacggtc agcgttggat ctgagagtgc gcaatcatca
    15661 gcggcaacag caaacaaggg aagtaaggtg agggcgagaa aaatccgttt gagattcaag
    15721 gtgatcattc ctttaccaat gagtagccga tgcgccatta taggtcctgg atgtgggatt
    15781 tttttatcct gttagcgacc ttgacgagta ccaaaaagcg cgaagttcaa ctattgttct
    15841 gtggtgttct gttgcgtgtt gacggcaaaa ttttgctggc gtaacatgcg cgcacgatca
    15901 ctctaagagg acattcgcct tggacacacc cagtagatac tggctcacta tcctgtcatc
    15961 caggatcaac tcctaaggct atcccttttt gctgatagcc ttagcggttg tcagcgacct
    16021 caatttttcc cgtcgcgctg agtcaggctg tttaatggtc tgaaacccaa tttgtttctg
    16081 tgtgcccacc gaactgtccg atattttaag cattgggagt cccggtcatg ctgagcgcat
    16141 ttcaactgga aaataaccga ctgacccggc tggaagtcga agagtcacaa ccccttgtaa
    16201 atgcagtatg gattgatctt gtcgaaccgg acgacgacga gcgactgcgc gtacaatctg
    16261 aacttggcca gagcctggca acccgcccgg aactggaaga catcgaagca tcggcacgtt
    16321 tctttgaaga cgatgacggc ctgcatattc actccttctt cttctttgaa gatgcggaag
    16381 atcacgccgg taactccact gtggcattta ccatccgtga tggtcgtctg tttactctgc
    16441 gtgagcgtga actgcccgct tttcgtctgt atcgtatgcg tgcccgtagc cagtcgatgg
    16501 tagacggtaa cgcctacgag ttgctgctgg atctgttcga aaccaaaatc gaacagttgg
    16561 cagatgaaat tgaaaatatc tatagcgacc tggagcaatt gagccgggtg attatggaag
    16621 ggcatcaggg cgatgagtac gacgaggcgc tctccactct ggcggaactg gaagatatcg
    16681 gctggaaagt gcgcctgtgt ctgatggata cccagcgcgc gctcaacttc ctggtacgta
    16741 aagcacgctt accgggtggg caactggagc aggcgcgtga aatcctgcga gatatcgaat
    16801 ccctgctgcc gcataacgaa tccctgttcc agaaggtgaa cttcctgatg caggcggcaa
    16861 tgggttttat caacatcgag cagaaccgca tcatcaaaat tttctcggtg gtatccgtcg
    16921 tgttcctgcc gccaacgctg gttgcttcca gctacgggat gaactttgag tttatgccag
    16981 aactgaagtg gagtttcggc tacccaggcg cgattatctt tatgatcctc gcgggcctgg
    17041 caccgtatct gtactttaag cgcaagaact ggctgtaact atacgatgtc gctgtgagta
    17101 agttagtgtg ctgacagcga cattttagca gagggaatcg cagataacat aattgttgtg
    17161 aataatatcc atgccacgtt gagttggacg cttaatgact cacatctcac cccttcaatt
    17221 gctgcagaga taatagaaaa aagtgtcaaa cccaatgaaa t
//
LOCUS       DBFIFI010000086        17230 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.086, whole genome shotgun sequence.
ACCESSION   DBFIFI010000086 DBFIFI010000000
VERSION     DBFIFI010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17230
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.086"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            172..966
                     /gene="yjfZ"
                     /locus_tag="N7B39_002704"
     CDS             172..966
                     /gene="yjfZ"
                     /locus_tag="N7B39_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HGU2422658.1"
                     /translation="MTLPTTIYSFPAYLSRFSSTDKPVKLKFHQYARATLLSNRGRDH
                     NCDGRRTVEIHKLDLSDWQAFNKLATKCNAYDGVTMNGDNSFGWNHEATLDNIHAQKY
                     NKAYAGARLTAELKYLLQDVESFEPNSKYTIHEVVLGPGYGTPDYTGQTIGYVVTLPA
                     QMPNCWSSELPTIDLYIDQLRTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSSS
                     AAIYSGLGFERVPQERDISCARMYLTPANHPDLWTQENGEWIYLRN"
     gene            complement(1037..1486)
                     /gene="rplI"
                     /locus_tag="N7B39_002705"
     CDS             complement(1037..1486)
                     /gene="rplI"
                     /locus_tag="N7B39_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HGU2422659.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(1528..1755)
                     /gene="rpsR"
                     /locus_tag="N7B39_002706"
     CDS             complement(1528..1755)
                     /gene="rpsR"
                     /locus_tag="N7B39_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HGU2422660.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            complement(1760..2074)
                     /gene="priB"
                     /locus_tag="N7B39_002707"
     CDS             complement(1760..2074)
                     /gene="priB"
                     /locus_tag="N7B39_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HGU2422661.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            complement(2081..2476)
                     /gene="rpsF"
                     /locus_tag="N7B39_002708"
     CDS             complement(2081..2476)
                     /gene="rpsF"
                     /locus_tag="N7B39_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HGU2422662.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            2803..3078
                     /gene="yjfY"
                     /locus_tag="N7B39_002709"
     CDS             2803..3078
                     /gene="yjfY"
                     /locus_tag="N7B39_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HGU2422663.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            complement(3207..3893)
                     /gene="ulaF"
                     /locus_tag="N7B39_002710"
     CDS             complement(3207..3893)
                     /gene="ulaF"
                     /locus_tag="N7B39_002710"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HGU2422664.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(3893..4747)
                     /gene="ulaE"
                     /locus_tag="N7B39_002711"
     CDS             complement(3893..4747)
                     /gene="ulaE"
                     /locus_tag="N7B39_002711"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HGU2422665.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAVEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            complement(4757..5407)
                     /gene="ulaD"
                     /locus_tag="N7B39_002712"
     CDS             complement(4757..5407)
                     /gene="ulaD"
                     /locus_tag="N7B39_002712"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HGU2422666.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            complement(5421..5885)
                     /gene="ulaC"
                     /locus_tag="N7B39_002713"
     CDS             complement(5421..5885)
                     /gene="ulaC"
                     /locus_tag="N7B39_002713"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HGU2422667.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQEAVKIGVDLLVAADVVEPRYY
                     QAILDGVEQFGPYFVIAPGLAMPHGRPEEGVKKTGFSLVTLKKPLEFNHDDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(5895..6200)
                     /gene="ulaB"
                     /locus_tag="N7B39_002714"
     CDS             complement(5895..6200)
                     /gene="ulaB"
                     /locus_tag="N7B39_002714"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HGU2422668.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKEHFPQDVK"
     gene            complement(6216..7613)
                     /gene="ulaA"
                     /locus_tag="N7B39_002715"
     CDS             complement(6216..7613)
                     /gene="ulaA"
                     /locus_tag="N7B39_002715"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HGU2422669.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIILGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            7968..9032
                     /gene="ulaG"
                     /locus_tag="N7B39_002716"
     CDS             7968..9032
                     /gene="ulaG"
                     /locus_tag="N7B39_002716"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HGU2422670.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            9140..9895
                     /gene="ulaR"
                     /locus_tag="N7B39_002717"
     CDS             9140..9895
                     /gene="ulaR"
                     /locus_tag="N7B39_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HGU2422671.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(9892..10641)
                     /gene="yjfP"
                     /locus_tag="N7B39_002718"
     CDS             complement(9892..10641)
                     /gene="yjfP"
                     /locus_tag="N7B39_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HGU2422672.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAEEK
                     WLLDDRLAVGGASMGAMTALGITARHPTVRCTASMMGSGYFTSLARSLFPPLIPETVA
                     QQNEFNNIVAPLAEWEATNHLEQLSDRPLLLWYGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            10823..11152
                     /gene="bsmA"
                     /locus_tag="N7B39_002719"
     CDS             10823..11152
                     /gene="bsmA"
                     /locus_tag="N7B39_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HGU2422673.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTLQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            11301..11576
                     /gene="yjfN"
                     /locus_tag="N7B39_002720"
     CDS             11301..11576
                     /gene="yjfN"
                     /locus_tag="N7B39_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HGU2422674.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(11693..13318)
                     /gene="aidB"
                     /locus_tag="N7B39_002721"
     CDS             complement(11693..13318)
                     /gene="aidB"
                     /locus_tag="N7B39_002721"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HGU2422675.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDCAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(13402..14565)
                     /gene="yjfC"
                     /locus_tag="N7B39_002722"
     CDS             complement(13402..14565)
                     /gene="yjfC"
                     /locus_tag="N7B39_002722"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HGU2422676.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(14568..15206)
                     /gene="yjfM"
                     /locus_tag="N7B39_002723"
     CDS             complement(14568..15206)
                     /gene="yjfM"
                     /locus_tag="N7B39_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HGU2422677.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(15216..15614)
                     /gene="yjfL"
                     /locus_tag="N7B39_002724"
     CDS             complement(15216..15614)
                     /gene="yjfL"
                     /locus_tag="N7B39_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HGU2422678.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(15632..16291)
                     /gene="yjfK"
                     /locus_tag="N7B39_002725"
     CDS             complement(15632..16291)
                     /gene="yjfK"
                     /locus_tag="N7B39_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HGU2422679.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVVAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKENHWREAINAKTMGAMTLNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(16342..17040)
                     /gene="yjfJ"
                     /locus_tag="N7B39_002726"
     CDS             complement(16342..17040)
                     /gene="yjfJ"
                     /locus_tag="N7B39_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HGU2422680.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARTLEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVAATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(17059..>17230)
                     /locus_tag="N7B39_002727"
     CDS             complement(17059..>17230)
                     /locus_tag="N7B39_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HGU2422681.1"
                     /translation="MMPLSSVGISSVQQEEYYIVFGALSLKSSLEDILLEITSLVDNA
                     LDLAEITEEYSH"
BASE COUNT         4380 a         4598 c         4120 g         4132 t
ORIGIN      
        1 tctccccggg tgcatcgtta tatccataat gagcgatttt tgatattaat atctttcacc
       61 atccgctatt ttcaccactt cagaaaacaa acttattttt attaatatcg atttttttct
      121 gtgaacattc agctgcgata tttataaaaa cattaaacag gtcacattac aatgacatta
      181 ccaaccacta tttattcatt tcctgcatat ttatctcgtt tttcgtccac cgacaaacca
      241 gtaaaattaa aatttcatca atacgcacgc gctacattac tttcaaatcg tggaagagat
      301 cataattgcg atggcagacg tactgttgag atccacaaac tagatctctc agactggcaa
      361 gcattcaata agttagcaac gaagtgtaat gcttacgatg gagtaacaat gaatggtgat
      421 aattcttttg gatggaacca tgaagctaca ttagataata ttcacgccca gaaatacaat
      481 aaagcttacg cgggtgcgag gttaaccgca gaactgaaat atttattgca agatgtcgaa
      541 tccttcgaac ccaacagtaa atacacaata cacgaagtgg ttttgggacc tggttatggt
      601 actccagact ataccggcca aacgatagga tacgttgtaa cccttcctgc ccaaatgcct
      661 aattgctggt catctgaact accaacaatc gatctttata tcgaccaact tcgcactgtt
      721 acaggcgttt ctaatgccct cggattcatc attgctgcat tgctaaacgc gtattctgat
      781 ttaccccatg atttgaaaat tggtctgcgt tccttatcaa gttcagccgc aatctactcg
      841 ggtttgggtt tcgagcgagt gccgcaggag cgagacatca gttgcgctcg tatgtatcta
      901 acgccagcaa accatcctga cttatggaca caagagaatg gtgaatggat ctacttgcga
      961 aactaatacc tgacgaaaaa tagaaagcaa aacgccgacc agtggtcggc gtttttacgt
     1021 ctcgttgaat aacgaattat tcagctacta cgtttacgat cactttcgcg aatacttcgc
     1081 tgtgaacctg gaagctcact tcgtgttcgc cagtggtacg cagaacgccg ttcggcagac
     1141 gaacttcgct cttagccact tcaacgccag ctgcagttac agcgtcagcg atgtcgcgag
     1201 taccgatgga accgaacagt ttaccttcgt cgccagcttt agacgcgatg gtaacagttt
     1261 ccagtgcatt gattttctca gcgcgagcat tagcagctgc cagaacttca gccagtttag
     1321 cttccagttc agcgcgacgt gcttcgaaga attcaatgtt tttcttggta gctggaacag
     1381 ctttaccctg tggtaccagg aagttacgag catagcccgc tttaacgttt acctgatcac
     1441 ccaggctacc caggtttgct actttatcaa gcagaataac ttgcattacc ttatcctctc
     1501 aaagtcgtat taatggaccg tgaccgatta ctgatggcga tcagtgtacg gcagcaggga
     1561 caggtagcga gcgcgtttga tagcgcgagc cagctgacgc tggtattttg cacgggtacc
     1621 ggtgatacgg cttgggacaa tcttaccgct ttcggtgatg tagtttttca gcgtagcgat
     1681 atctttatag tcgatctctt gaacgccttc cgcggtgaaa cggcagaact tgcgacgacg
     1741 gaaataacgt gccatatggc tagtctccag aatctatcaa ttcaatctgc tcggcatgca
     1801 aaaccatttt gctcagtccg ttctttgcct tgtggcatga aatgaacccc tgaacggtta
     1861 tgcgactgcc gaccgttata ctgtgagtaa tggcctggtt ttcgtgtccg ctaacaataa
     1921 cgggcatttg acaccatgcc tgccggtgaa agccggcttc ctcctgcaca gaacgatgct
     1981 caagcacgaa ctggcagtga ggaattcctg atggactgac ctttcgaagg ggagtcctgc
     2041 acacggtgcc ggacaacacc agacggttgg tcatcagaaa ttactcttca gaatccccag
     2101 cttcagcatc atcagcggtt tcgtttgcga aatcatcgcg acgctcacgg cgctcgtctt
     2161 tcgctttaac catcggagat gcttcggtaa cagcgtgctt ggtacgcata accatgctgc
     2221 ggataacggc atcgttgaag cggaaggtag tttccagctc atcgatcact tcctgcggag
     2281 cttcaacgtt catcagaacg tagtgagctt tgtgcagttt gttgatcggg taagccagct
     2341 gacggcggcc ccagtcttcc agacggtgga tcttgccttc tgcaccagtg atggcagcag
     2401 tgtagcgctc gatcatgccc ggaacctgtt cgctctggtc aggatggacc ataaaaacga
     2461 tttcgtaatg acgcatcgaa ttgctcctta cggattattc agcctcctgt ctgggtcagc
     2521 cgaatcccgg ggaggcaagg aacgtgttaa aggtcggctg aaaaatgacg cgttatagta
     2581 ctgacatacc ccgctaaact caaggccagc cgacaaataa atctcatctt tcaccaaagt
     2641 ggactaacac gctgatttga agttcatcac acttcatcat ggtgtttttt cgaacaactg
     2701 catcagaaat ggtcaccatc aacttcttta tcattgatta aaattaaatc agaagatcat
     2761 cacagaggag agcacacaaa gtcagccgtc gaggaggtat ctatgttcag tcgagtttta
     2821 gcccttctgg ctgtgctttt gctaagtgca aatacatggg cagccattga aattaataac
     2881 caccaggcca gaaatatgga cgatgtgcaa agcttaggcg tgatttatat caatcataat
     2941 ttcgccactg aaagcgaagc acgtcaggca ttaaatgaag aaacagatgc gcaaggcgca
     3001 acgtactacc acgtaattct gatgcgggaa ccggggagta acggcaatat gcacgccagc
     3061 gcggatattt atcgctagca ccaggtataa ccaacgaaac attgccatag tttgctttgc
     3121 ccccttcgca gggggctttt tttgaaaaac ggctgcggaa ttagaccagt tatctcccga
     3181 ggaaggaaat ttccgcagcg cgtgttctac ttctgcccgt aataagcgtt ggggccgtgc
     3241 ttacgcatga agtgtttatt catcaggaag ctgtcgatgt gattgagttg tggattaata
     3301 ctgcgcgcaa tccacgccat tttcgccact tcttccatca ctaccgcgtt atgcaccgcg
     3361 tcgtgggcat ctttccccca ggcgaacggt ccgtgctgat acaccacaat tcccggcgta
     3421 tgcagcggct cggcgttgcc cagcgtttcg ataatcactt tgccagtgtt cagttcatac
     3481 tcaccctgca cctcttcttc gcttaatcca cgcgtacacg ggatatcacc aaagaagtag
     3541 tcagcgtgcg tggtgcctaa cgccgggatc gccagccccg cctgcgccca tgcggtagca
     3601 tgagtggagt gggtatggac aatgccgcca agcgacggat aacgacggta gagttcgaga
     3661 tgcgtcgcgg tgtcggaaga cggacgatat tccccttcca ccaccttacc gctcatatcg
     3721 accaccacca tatcgtctgc tttcatggtt tcgtaggcga cgccgctggg cttgatcacc
     3781 accagcccgc gttcgcggtc gatagcgctg acgttgcccc aggtaaaggt caccagccca
     3841 tagcgcggca gatccatgtt ggcttcaaac acctgctgtt ttagcttttg cattatgccg
     3901 cctccaccat gcccgctttc gccatgcgcg ctttcaccca atcacgcgct ttcgccactt
     3961 ctgccgccgg gtcttccgcc gtttcgctcc acatctcaat caggtacggc ccgcaatagc
     4021 cactctgttt gagcgtttcg aagcaacgtt cgaaatccac cacaccttcg ccaaatggca
     4081 cgtttttgaa gacgcccggt ttggtgtctt tcacatgtac cgcgacgata tgcccgattc
     4141 cggcctgcaa ctccatctgc acatcgttgt cccacgccga caggttgccg atatccgggt
     4201 agagctggaa ccacggattg ttgagatagt gcgcgtagcc cagcgccttg ctgatggagt
     4261 tcatcaacgg ataatccatg atctccaccg ccagcgtcac ctgcgcgcgg ctcgccatct
     4321 caacgctctc tttcaggccg tcacggaaac gacgacgcgt ttcgttattg gcttcctgat
     4381 agtaaacgtc atagcccgcc agttggatca cgcgaatacc aacgtcctgg gcgaactgga
     4441 tagctttacg cataatctcc agcccctgcg cccgcaccgc gtcatcttca ctgcccagcg
     4501 ggaaacgacg atgagcagaa aggcacatgg acggcacgcg cacgccggtt tcaacaatcg
     4561 cattcaccag cgccagacgc tgctcgcggc tccagtcgag gcgcgacagg cgctcgtccg
     4621 tttcatctac cgacatctcg acaaaatcga agcccaacgt ttttgccagt tgcaggcgtt
     4681 ccagccagca ctccccggcg gggagcgctt tttcatagat gccaagcggg atttgtttgg
     4741 acaacatatc cgctccttag ccccacagtt cggcgatgga acgtttgaac tgacgtgcgg
     4801 cttccaccgg agaagcggca tcacggatac tacggcccgc gataaagacg tgaatcggaa
     4861 tgcctttgaa cagcggcaga tcttccagcg ccaggccacc agtgacggtg actttgaagc
     4921 ccatatcgga aagacgtttg atcgcggtga tgtccgcttc gccccacgcc acgcctgcgg
     4981 cctgcgcgtc acggctgcgg tgataaacca cctgctgaat acctgcgtcg cgccactgtt
     5041 gcgcctgttc ccaggtccag taaccggtca gttcgatctg cacgtcgccg ttaaactctt
     5101 ttgccacgtc cagcgcgcct ttggcggtgt tgatatccgc acagcaaatt accgtcaccc
     5161 agtcagcgtt ggcttcgaag cacatacgcg aaaggatttt gcctgcatcg gcaattttgg
     5221 cgtctgccag tacgattttg tgcgggtaga gcgctttcag gtcacgaacc gcacgcacgc
     5281 cttcgcccac gcacagaatg gtgcccactt cgataatgtc gacttcttcg gcaatcaggc
     5341 gagtggtttc gtaggcgcta tccatagtct ggttgtccag cgcgacttgc aacatcggta
     5401 atgacatgtt caattccttc ttaagctgcc gcgttggtgc ggtcaatgag atccagtact
     5461 tcctgctcgg tacggcaggc gcgtaaacgg tcaaaattct cttcatcttc aaacaggttg
     5521 acgatctgca tgatgcccac ttcctggtga gtattggcat cgaccgccgc catggtgatg
     5581 aggatatcca ccggatcgtt gtcatcgtgg ttgaactcca gcggcttttt cagcgttacc
     5641 agagagaaac cggttttctt aacgccctct tccggacgcc cgtgcggcat tgccaggccc
     5701 ggagcgataa cgaaataagg accgaactgt tcaacgccat ccagaatcgc ctggtagtaa
     5761 cgcggctcta ccacatctgc cgccaccagc aggtcaacgc cgattttcac cgcttcctgc
     5821 catgtctctg cttcagcctg cagacggatg gatttatttt cagccagcga atcacgtaat
     5881 ttcatagcgc gtccttactt cacgtcctgc gggaaatgct ctttgatcac ttccagcagt
     5941 ttcgggccaa agtcggcagg agagagcatg ttgcgcacgc caaccacgta tttgttgccg
     6001 gtcacggtga tttcgcccgc aatgtgcgta gaagcgatga tgatatccgc gccactcaac
     6061 tcgcttttgt actcgccaac cgcgcagctg tttaccgtat ggtcaatgtt tgattgggtt
     6121 aaaaactggt ccactttcat cttcatgatc atggaactgc cttgtccgtt gccacacaca
     6181 gccagaatac gtacggtcat aatcaaaact ccttattaag cagactgttc tgccagttgt
     6241 ttttctgcat cttcttctgc gcgcagcgcg cggccagcga agaacatata agccagtgca
     6301 attacaatga tgacggccat aaaggcgata ccgatggaga agaagccttg catcatcggc
     6361 ggtgccagaa tcgaccagtc cgccatgccc atccaggcac tcataccggt gagtttcacc
     6421 gcccagacgc aaccaaagat ttcgatcatt cccatcacca gacaaatctt cagcgctgca
     6481 cgccagccgc cgaagtggtt agcgaacacg ccgatggtgg cgttggagaa gaacatcggg
     6541 ataaagccta gaataatcag aatggaagag ccgcaggcga ccaggatgcc aactgcaatc
     6601 agctgaccga tggtgcccca cataaagccc cagaccacgg cgttcggcgc gaagctatag
     6661 atagccgcac agtcaatcgc cagaaccgca cctgggatca ggcgctggga gataccgtta
     6721 aatgcttcag agagttccgc cacaaacatg cgcacgccct gagtgatgat gaagatcgcc
     6781 accgcgaagg agaaaccggt ttgcaggatg tagaccgtcc agtgcacttt gcccgccatc
     6841 gcctgcacgg tgtcgatacc gaaggagagc agaatggcgc caaagaagat ggtcatcaca
     6901 atcgccgtgg agacgatgtt gtcgtggaaa atgttcagcc agcccggcag tttgaggtct
     6961 tcaacgctct cctctttttt gccgaggaac ggcgcgactt tataggcaat ccatgatgca
     7021 aactgctgct ggtgaccgat ggagaaacca cagccatccg tcacttcctg agtcggcttg
     7081 tacatcatgt tggaagtgat gccccagtag agcgaaacca gaatcgcggt acagataatg
     7141 gtggtccaca tggagtagcc gaagataaac agcgtaacgg caatcaaccc ggcctgctgg
     7201 aacatgatgt ggccggtcaa catgattgtg cgaatgccgg taatgcgacg caacagcacg
     7261 taacagatgt tcagtgccag cgctaacaat acggcgtaac ccacccagct ataggcatcg
     7321 cccatgcggt cgatggttgc catcattgaa gcgtaggtat cggaaattgc gccgttaatg
     7381 ccgtagactt cggacatttt cgccaccacc ggtttgaagg tgctggtgag gatgccggac
     7441 cctgcctgca acaacatgaa accaattatg gttttaatcg tgcctttaat aataacgctg
     7501 acacttttgc gcagtaggat gtagcccaga caggtcacaa tacccagcaa caacggggca
     7561 ttggtcatga cctggttaaa aaatacggta aagatgttgt agaggatctc cataacgatc
     7621 tcctgaagaa gaggtaaccg ggtattccac acacccggaa tgttgtgcac tcactctaat
     7681 tttcaaaagt aatcacaaca agattattta tgattaaatg tgacgcgacc cgcaaataat
     7741 tccacatgga tagagcggga ttcacgcaac gaaacaatta ttcatttaaa ttcatttagt
     7801 taattgaatt atacattcca ttaaatcttc ccatactcaa aatcaacaac aacttccctg
     7861 gcaaaaagtc aaaaaatggt cattgccttc cagaaaaaat agtgtcagga tcaatcaaaa
     7921 ccaatcacat attgattcga attgaactat catgaaggta aatggcgatg agtaaagtga
     7981 aaagtatcac ccgtgaatcc tggatcctga gcactttccc ggagtggggt agctggttga
     8041 atgaagaaat tgaacaagaa caggtcgctc ctggcacatt tgcgatgtgg tggcttggct
     8101 gcaccgggat ctggttgaaa tcggaaggtg gcaccaacgt ttgcgttgat ttctggtgcg
     8161 gcactggcaa acaaagtcac ggtaacccgt taatgaaaca gggtcaccag atgcagcgca
     8221 tggctggcgt gaaaaaactg cagccaaacc tgcgtaccac cccgtttgtt cttgatccgt
     8281 ttgcgattcg ccagatcgac gcggtactgg cgactcacga tcacaacgat catatcgacg
     8341 ttaacgtcgc tgctgccgtg atgcagaact gtgctgatga cgtaccgttt atcggaccga
     8401 aaacctgtgt ggatttgtgg attggctggg gcgtaccgaa agagcgttgc atcgtggtca
     8461 aaccgggcga tgtagtaaaa gtgaaagaca ttgaaattca tgcgcttgat gctttcgacc
     8521 gtactgcact gatcaccctg cctgccgatc aaaaagcggc tggcgtactg ccagatggca
     8581 tggacgatcg cgcggtgaac tacctgttca aaacgcctgg cggctccctg tatcacagcg
     8641 gcgactccca ctactctaac tattatgcga agcacggtaa cgaacatcag atcgacgtgg
     8701 cgttaggatc gtacggcgaa aacccgcgcg gtatcaccga caagatgacc agcgccgata
     8761 tgctgcgtat gggtgaagcg ctgaatgcga aagtggttat cccgttccac cacgatatct
     8821 ggtcaaactt ccaggccgat ccgcaagaga tccgcgtgct gtgggagatg aaaaaagatc
     8881 gtctgaagta tggcttcaag ccgtttatct ggcaggtggg cggcaaattt acctggccgc
     8941 tggataaaga caacttcgag taccactatc cgcgcggttt cgatgattgc ttcactattg
     9001 aaccggatct gccgttcaag tcattcctgt aatctgtcgt ttataccgga tagcgtggag
     9061 aagttcgcgc tatccggcgt agtatttcct ggctatttca aatatcatct aaaaaaatca
     9121 aattttatcg gaatagctca tgactgaagc acaaagacat caaatcctcc tggaaatgct
     9181 cgcacaattg ggctttgtga ccgttgagaa agtcgttgag cgtctgggaa tttcgcctgc
     9241 cactgcgcga cgcgatatca ataaacttga cgaaagcggc aaactgaaaa aagtgcgcaa
     9301 tggtgcagaa gctattaccc aacagcgccc gcgctggacg ccgatgaatc tgcatcaggc
     9361 gcagaatcac gatgaaaaag tacgtatcgc taaagcggcc tcgcagctgg ttaatccggg
     9421 cgaaagcgta gtcatcaact gcggctccac cgcgtttctg cttgggcggg aaatgtgtgg
     9481 caagccagtg caaatcatca ctaattatct accgctggca aattacctga tcgatcaaga
     9541 acatgacagc gtgatcatta tgggcggaca gtacaacaaa agtcagtcca tcactttaag
     9601 cccgcagggc agcgaaaaca gtctctatgc cgggcactgg atgtttacca gcggaaaagg
     9661 gctgaccgca gaagggttgt ataaaaccga tatgctgaca gcaatggcag agcagaagat
     9721 gctgagcgtg gtagggaaac tggtggtact ggttgatagc agtaagattg gcgaacgcgc
     9781 gggaatgctt tttagccgtg ccgatcaaat cgatatgctt atcaccggca aaaatgctaa
     9841 cccggaaatc ctgcaacaac tggaagcgca aggggtcagc attctgcgtg tttaaagatg
     9901 ctggcggaaa aatgtcacgg cagcatctaa cgcctcaggc gtaatgcggt gacgcacgcc
     9961 tggctgccat gaacaggtta gcagtttatc ccgtcccgtc tcgcttaagg cctgctgcaa
    10021 acgtagtgat tcgtcggcag gcacaacgtc atcgtcgagg ccataccaca gcagtagagg
    10081 tctgtcacta agttgttcca ggtggtttgt cgcttcccac tctgccagtg gcgcgacaat
    10141 gttattgaat tcattctgct gtgctaccgt ttcaggtatc agcggtggaa acagtgaacg
    10201 ggcgagtgat gtaaaatagc ccgatcccat catgctggcg gtacatctca ccgtggggtg
    10261 gcgagcggta atccccagtg ccgtcatcgc gcccatcgac gcaccaccga ctgccagacg
    10321 gtcatcaagc agccactttt cttcggctat tgccgcacgt aaagtagtga attcctgcat
    10381 actttgtagc aagatttgcc agaattgatt taaccgccgc gctgcgtcac cactaaaacg
    10441 gctaccgtga tcgggcgcat ccggcatgat cacccgcaaa ccagcctgcg ccagcgcaac
    10501 ggcaaaatag ctatacacca gactggacga agtaaagccg tgataaaaaa tcacgcacgg
    10561 taacggggta tctttttgtc cgacaggata agcatgaaga acgggaatat ctgccagctc
    10621 gcgtgattct atttcaatca tctgtctctc ctttttgtct ggtatgacat gaagaaatcc
    10681 ttagcacaag tttctgctgg cggtattagt tcataatgtt gagatgtggg ttacgctttc
    10741 gtcacgtttt cattcgaaaa tcgcgtacca ggtaacaatt cgggaacatt cccccaaagt
    10801 taaattaata aggcactaca ctatggttag caggaaacgt aatagcgtta tttaccggtt
    10861 tgccagttta ttattggtgt tgatgttaag tgcctgtagc gcactgcaag gtacgctaca
    10921 gccagcaccg ccagtaacgg accatccgca agagattcgc cgcgaccaga cgcaagggtt
    10981 acagcgaata ggtagcgtaa gcacaatggt tcggggttct ccggatgacg cattagcaga
    11041 aatcagagca aaagctgtcg ctgcaaaagc tgattattac gttgtcgtca tggtggacga
    11101 aaccatcgtg acaggacagt ggtattcaca agccattttg taccgtaaat aaatcagaca
    11161 acttacatag tcagctttac attgctttgc gtccctatgc gtttacctgt gcacaataaa
    11221 ttaccggctg gaagcctggg cgtttattac cgcgatggaa tgccctgcaa catgtgggga
    11281 aacgaaaaat ggagctgacg atgaaacaat tacttgcctc accctcgctg caattagtga
    11341 cttatcctgc gagcgccacg gcacagtctg cagaattcgc cagtgctgac tgcgtgacgg
    11401 gcctgaatga aataggtcag atctcagtta gcaatatttc aggggatccg caggacgtgg
    11461 aacgtatcgt ggcgttaaag gccgatgaac aaggtgcgtc atggtaccgc attattacca
    11521 tgtacgaaga ccagcagcct gataactggc gcgtacaggc gatactgtat gcatgagtgt
    11581 catctacagc aaatgaacaa tgccggatgc ggcgcgagcg ccttgtccgg cctacaattt
    11641 tcacatattt catttagtta atcgaaacca gcgtcgcatc agtcgtatac gcttacacac
    11701 acactccccc cgtcgcccgc agcaataaat cattctggat ctgctctgac aaccgtacgc
    11761 cgccgcgcgt atctaacatc acctgacacc acgcctgcgc cattggcgga gaagcatatt
    11821 tcaacatttg cgcaccgcag cccagcagga atagctgatg agtaatctct cgccccagtt
    11881 cttcagctgg cttacgcagc tgctgctgta aacgacgaac cgcgcaatca aaatagcgat
    11941 cctgcccttt cacttctaca aatgcttccg acaataagtc gtatacgccc gcttgcttat
    12001 tgagaacgcg cagcacatcc aggcacataa tattgccgga accttcccaa atactgttta
    12061 ccggcatctc ccggtaaagc cgcggcagct cgctctcctc gcaataacca atgccgccca
    12121 gcacctccat cgcttcggcc acaaacggca tacctcgttt gcagatcaca aattttgccg
    12181 caggcgtaaa taaacgcgcc cacagagctt ctttggcatc ggcgcgccgg tcccacgctc
    12241 gcgcaagacg aaacagcaac gccgtttgcc cttcaagctg aagtgccatg cgacttaaga
    12301 catgacgcat aaggggctgt tggatcaatg gattaccaaa aacatggcgt tgatgcgcat
    12361 gataaatcgc cagcgagaac gcacggcgca tcatggcatg gctacccagg gcgcaatcaa
    12421 aacgcgtcat cccccccatt ttcaggatca gacgaattcc ttccccttcc tgccccaata
    12481 accagccaat ggcatcctga aactccactt cacaactggc gttagagcga ttacccagct
    12541 tatctttcag ccgctcgagg cgaatcgcgt tgcgttgccc gtcaggcaaa aagcgcggca
    12601 caaaaaagca ggacaaaccg cccgcggtct gcgccagcac cagatgcgca tcgctttgcg
    12661 gaactgagaa aaaccattta tgccccacca gccgataaga gccatcctcc agacgctctg
    12721 cgcgggtggt gttgctcatg acatcggaac cgccctgctt ttccgtcatt cccatgccaa
    12781 tcaacaaacc gcgtttttgc ccacctggca ataagtgaga atcgtagcga tcgctcaaca
    12841 gcggtgtggt ccagtcctga aacggcgcgg gtaacatctg taacaacaat ggcgtggcgg
    12901 caaaggtcat ggttatcgga cataacgacc ccgcctcaac ctgtgcgtgc aacataaaac
    12961 gcgccgcgcg cgccacaaat gcgccggagc gagcgtcttc ttcccaggca agattgtgca
    13021 cccgattggt acatagtgcc tgcatcagca ggtgccaggc ggggtgaaaa cgcacatcgt
    13081 ccagacggcg tccttgcgca tcgtagcgca acagttcagg cggattcaca ttcgccagcc
    13141 gccccagttc aagggattca gccgttccta actgctgacc aatactggct agaaaatcgc
    13201 tatcccagcc agcaccttca cgcgttaccg cttcgcaaag cgcgccatca gacaggtata
    13261 agttgctgtt atttaatggt ataggttgat taaaaacggt gtgagtttgc cagtgcactg
    13321 tgtctccctc catcaatggc agtcaccatt agtatggtca ctgccatgga ttcatgacag
    13381 aaagagattg ctaaaacatt cttatccagc aatgtaatgc ggaacgaaac gtgaggtgtc
    13441 tttggtgatc agtgtgttat cttcacggat ccccattccg cacgcttcat catcgacaat
    13501 ccagctaccg atgagtgtgt agctatcgcc aaaccgcggc agaggttgaa acgcctggta
    13561 gatcatcggt tcatcggcgt aatcaccatc agcgtggtca acgacgttat tctggccgtc
    13621 gaaaatggtg acatttccgc cttcgcgcga gtagatcggt ttacgcacat agctttcgcc
    13681 agcggcaatc tgcggttttt caccctcaaa ccaggatgca agaaggttag gatgaccagg
    13741 gaagaagcgc caaagcagcg gcattagccc tttattactc aagatacttt tccataacgg
    13801 ctccacccat tgctcgcgac gcttgcgcag cagcggaccg ttatcgtcac gcatcatcca
    13861 ctccagcgga tacagcttaa atgcacgctg gatgacatta tcatcaagat cggtcagtac
    13921 gccgccgacg cccaaaccga gatcttcaat gtagataaac cgcgactcct gccctgcctg
    13981 ctgggcgcag tcctgcaagt acagcacggt actcctgtct tcatcggtgt cctgacagca
    14041 gcaaaaataa aacggttccc gactgtaaag ctcgctaaag cgtgaaatca ggcgttcctg
    14101 aatagcattg tactgatcgg catcacgcgg aataatgccg ctgcgccggg catcctccag
    14161 ccacagccac tggaaataag ccgactcgta caatgaagtt ggcgtatcgg cgttgtactc
    14221 cagcagcttc accggcgcat tgccacacca ggcaaaatcc atccgtccat acagcgaagg
    14281 atcgcgggcg cgccagcttt cagcgatcac atcccagtac aacggcggga tcgccagttg
    14341 cgtcaggatc tcttcatctt ttaccgcgcg atccaccacc tcaaggcaca tctgatgcag
    14401 ttccgcagtc ggtttttcga tctgctcttc aatctggcgc agagtaaaac ggtaagcccg
    14461 actctcatcc caatagattt cattgtcgat gatatgaaag tcgaaaccgt tgtcagcggc
    14521 gatctggtcc agatcccgtc gcacaggaac gttgtgtctc agcatgatta gcctccccaa
    14581 tgcccacggg cgctggaaga acgaccatag ccgccgcgag aaacggttga cgcttttttg
    14641 gtggtaaagc cgcctgaaga gtaagactct tttttgccag aaccggagcg ccaggagtaa
    14701 tcaccggaag tgttgcgcca gacggggcgg gaagcaaatg aggatccgcc gctgttatag
    14761 acaaacggct catcgcgatc tttacgaata acccggctta acaaaaatcc agaaacgacc
    14821 gggatccaac tctgttcaac attgtcgtag tagcaatttt cgtacttaga ctgacagttc
    14881 tgctgagtca tattcttcgg aacatcggca taaaatgccg ctttggcgtt gttccagcca
    14941 cgcgcgcaaa tgtcggcatt attaccgtca tcaatacaat cctgcactgt cgaataaaac
    15001 gttccgtcgc cgtcgttatc gacatcgcca ctatcgccac aacctttcaa tacaaaaaaa
    15061 gcggcaccgc ccattagcgc caacgttaag tatttttgtg cgtagcgatt gcgacgcggt
    15121 tcaaaaggat tattcggtct accaatgcga ctgatcgctc cgtgaccaat tttactgtta
    15181 tttcttgatt tgcgttttct ggccatgatc gtccattacc atgtcataca agctgcgtta
    15241 aaaataccgc cagccagcgc ggcggttccc ataaacattc ctgctgcggt attgtgatta
    15301 ataatttttt cgcttaatgc gggcatataa agtctgacac cagcaaaaac gagtaactga
    15361 atcaccagtg caatcccgcc ccaggcgaaa tagtctggaa tactcaccgc attgattgcc
    15421 gcactggata aggggatcac gtaacccaac aatgtaccgc tgaacgccag tgacgctgcg
    15481 gtattattgt ttttgattaa ttgccattca ttgtgcggtg taattttgga gtagataaac
    15541 agaaaaataa tcaccatagc cacgccaata aaaaaatagg cactaaaggc aagaagtgaa
    15601 tccagtatat gcattgtaaa cgtccttgta attaaccaat aatatgaagt gatgtcagcg
    15661 ggatatcgac acccaacgcg cgcgaaaata accactctgg ctcgccgaga tcgttaaaag
    15721 attcctcacc atttagcagc aaatattcgt aagtatcttc ggtcacttgc cgctggtagc
    15781 ccatggtaaa attatggact tcccatttag catgattttg attttctact ttttccagca
    15841 tgtagacggg ttcgatattt cccggttctt cgctgttaaa aaatcgctgc cagcgttttt
    15901 cctgccagtt taaggtcatt gcccccatcg ttttggcgtt gatcgcctcg cgccagtgat
    15961 tctctttact gataccgtag ctctcttcat agacaaagag cttgatatca tcaatgtcat
    16021 caatatcttc gccgccggtg gtattgattt gcagaaactc atcgcctgaa gtgtagtagc
    16081 ggaaaatctg actaccgccg cccagatcga tgtggctgac agcgactacc gtaaattcct
    16141 cacccggcag cgcaatcagc aattcatctt ccagcaaacg aaacgctaac gtgtcgagcg
    16201 taaagccact attgagatga agtcccagcg gaccgcgagc gatagcaggc ttattatcct
    16261 tgccaaacag acgctggaaa aaaccagaca tattttatcc ttaaaatagt tgccagcctt
    16321 gcagtgaggc tggcaaaaaa attactcgcc ctgttggcgt tgcagtcttg ctaatacatc
    16381 ctgggcgcta cttttattgc taccgccaat tccggcttcc gccagctttt cgtcaaggtc
    16441 gcgaccgtct gcgacttttt ctaactgtgc ggcagcatcc aggcgagcct gacgttcggc
    16501 ctgacgcgtt tgcaggcgtt ttaaggattc tgctgctgtc gaaacgctgg aagatgcgcc
    16561 aacggtagaa gtcgttacag cctgttgtgc acgctgcatg gcttcagtgg ctttaacgac
    16621 ttccatttgc tgctcaaact gagcgatacg ctgcgctgtc gctgcaactg ctttttccac
    16681 gccatcacgg gagacttcca gattcgacaa cacttgctct tcagcggtaa tgagattctc
    16741 aaggcgcgcg atttcttcag caacttcgtt aatcaacgac ggattaacgt tcttgcttaa
    16801 cgcttccagc gtacgggctt ccagactggc tttgcgctca cgtaaatctt tcagcttatc
    16861 gtgacttaat ttcacccgcg ccagcagatc aacgcgagat tttccggctt tatcgagttc
    16921 agctttagcg tcacgaatat gctgttccag catgcgcaca ccctgggttt cttcaatgga
    16981 ttcttccgcc tgggagataa acgatttccc cagcgtaaat aaacttttta aaattcccat
    17041 aaatacccgt ccttgtaatt agtgtgaata ttcttctgta atttcagcca gatccaatgc
    17101 gttatctacc agcgaggtta tctcaagcag gatatcttca agagaagatt taagcgataa
    17161 cgccccgaaa acaatgtaat actcttcttg ttgtacgctg gagattccta ccgatgacaa
    17221 cggcatcatc
//
LOCUS       DBFIFI010000087        17136 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.087, whole genome shotgun sequence.
ACCESSION   DBFIFI010000087 DBFIFI010000000
VERSION     DBFIFI010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.087"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            135..1274
                     /gene="arnB"
                     /locus_tag="N7B39_002728"
     CDS             135..1274
                     /gene="arnB"
                     /locus_tag="N7B39_002728"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HGU2422682.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNHALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLQGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADINAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENIARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPSLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1278..2246
                     /gene="arnC"
                     /locus_tag="N7B39_002729"
     CDS             1278..2246
                     /gene="arnC"
                     /locus_tag="N7B39_002729"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HGU2422683.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHILVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREYGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2246..4228
                     /gene="arnA"
                     /locus_tag="N7B39_002730"
     CDS             2246..4228
                     /gene="arnA"
                     /locus_tag="N7B39_002730"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HGU2422684.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHHCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            4225..5115
                     /gene="arnD"
                     /locus_tag="N7B39_002731"
     CDS             4225..5115
                     /gene="arnD"
                     /locus_tag="N7B39_002731"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HGU2422685.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKYHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQQVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            5115..6767
                     /gene="arnT"
                     /locus_tag="N7B39_002732"
     CDS             5115..6767
                     /gene="arnT"
                     /locus_tag="N7B39_002732"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HGU2422686.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLTIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            6764..7099
                     /gene="arnE"
                     /locus_tag="N7B39_002733"
     CDS             6764..7099
                     /gene="arnE"
                     /locus_tag="N7B39_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HGU2422687.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAISKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            7099..7485
                     /gene="arnF"
                     /locus_tag="N7B39_002734"
     CDS             7099..7485
                     /gene="arnF"
                     /locus_tag="N7B39_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HGU2422688.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(7479..7745)
                     /gene="pmrD"
                     /locus_tag="N7B39_002735"
     CDS             complement(7479..7745)
                     /gene="pmrD"
                     /locus_tag="N7B39_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HGU2422689.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASCYSPDEWERQCKVAGKTQ"
     gene            complement(7855..9210)
                     /gene="menE"
                     /locus_tag="N7B39_002736"
     CDS             complement(7855..9210)
                     /gene="menE"
                     /locus_tag="N7B39_002736"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HGU2422690.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGF
                     AVQGVVEDSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHVATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQTEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVMEYDHESVDLSEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(9207..10169)
                     /gene="menC"
                     /locus_tag="N7B39_002737"
     CDS             complement(9207..10169)
                     /gene="menC"
                     /locus_tag="N7B39_002737"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HGU2422691.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELADTLPQAA
                     NYRTAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(10169..11026)
                     /gene="menB"
                     /locus_tag="N7B39_002738"
     CDS             complement(10169..11026)
                     /gene="menB"
                     /locus_tag="N7B39_002738"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HGU2422692.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(11041..11799)
                     /gene="menH"
                     /locus_tag="N7B39_002739"
     CDS             complement(11041..11799)
                     /gene="menH"
                     /locus_tag="N7B39_002739"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HGU2422693.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCLEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILSFWLVGYSLGGRVAMMAACQGLAG
                     LCGVVVEGGHPGLQNAEQRAERQRSDRQWAQRFRSEPLTAVFADWYQQPVFASLNDDQ
                     RRELVVLRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11796..13466)
                     /gene="menD"
                     /locus_tag="N7B39_002740"
     CDS             complement(11796..13466)
                     /gene="menD"
                     /locus_tag="N7B39_002740"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HGU2422694.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIACRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPKSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETVLADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13555..14850)
                     /gene="menF"
                     /locus_tag="N7B39_002741"
     CDS             complement(13555..14850)
                     /gene="menF"
                     /locus_tag="N7B39_002741"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HGU2422695.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAREFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIAHHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14929..15234)
                     /gene="elaB"
                     /locus_tag="N7B39_002742"
     CDS             complement(14929..15234)
                     /gene="elaB"
                     /locus_tag="N7B39_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HGU2422696.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15289..15750)
                     /locus_tag="N7B39_002743"
     CDS             complement(15289..15750)
                     /locus_tag="N7B39_002743"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU2422697.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLDT
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEIYEEDGIPHIGMAREVIQA"
     gene            15815..16732
                     /gene="rbn"
                     /locus_tag="N7B39_002744"
     CDS             15815..16732
                     /gene="rbn"
                     /locus_tag="N7B39_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HGU2422698.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPSPLFQELKAGKTIMLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            16920..>17136
                     /locus_tag="N7B39_002745"
     CDS             16920..>17136
                     /locus_tag="N7B39_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HGU2422699.1"
                     /translation="MMVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDE
                     ESIELLHNIALGYDEFGKKAEDILYHIV"
BASE COUNT         4003 a         4623 c         4361 g         4149 t
ORIGIN      
        1 ttacgtctaa tattagtttc ttaaggttaa gttaatattc tatccttaaa atttcgctct
       61 aaatggcaaa atatacacaa cactctttat agcaaatata agtggacagg tattcaatgg
      121 cggaaggaaa agcaatgtca gaatttttgc ctttttcgag accagcaatg ggcgtggagg
      181 aactcgctgc agttaaagag gttctcgaat ccggttggat cacaaccggt ccgaagaatc
      241 acgcgcttga acaagccttt tgccagttga cgggaaatca gcatgccatc gcggtcagtt
      301 cagccaccgc cggaatgcat atcacgctaa tggcgttgga aattggcaag ggcgatgaag
      361 tgattacgcc ttccctgacc tgggtttcaa ccctcaatat gatttccttg cagggtgcaa
      421 cgccggtaat ggtggatgtc gatcgcgata cgctgatggt cacgcctgaa gcgatcgaat
      481 cagccattac gccacgcact aaagccatca ttccggtgca ttatgccggt gcgccagcag
      541 atattaacgc cattcgcgcc attggcgaac gttacggcat cgcagttatc gaagatgctg
      601 cccatgccgt aggtacgtat tacaaagggc gacatattgg cgcaaaaggt accgctattt
      661 tttcatttca tgccattaaa aatattacct gtgcggaagg aggcctgatt gttactgata
      721 atgaaaatat tgcccgccag ctacggatgc tgaaatttca cggcctgggt gtcgatgcct
      781 atgacagaca aacctggggc cgtgcaccgc aggctgaagt cttaacgccg ggctataagt
      841 acaatctgac cgatattaac gccgcgattg ccctgacaca gttagccaaa ttagagcacc
      901 tcaacacccg tcggcgcgaa attgcccagc aatatcagca agcactggca gctctcccct
      961 ttcagccatt aagccttccc gcctggccgc acgttcacgc ctggcatctg tttattattc
     1021 gtgtcgatga acaacgttgt ggtatcagtc gcgatgcgtt gatggaagcg ttaaaagaaa
     1081 gaggtattgg taccgggtta catttccgcg ccgctcacac acaaaaatat tatcgcgagc
     1141 gttttccctc gctgtcgtta ccgaataccg aatggaatag cgaacgcatc tgttctttgc
     1201 cgctgttccc ggatatgact accgccgatg ccgaccgtgt catcacagcc cttcagcaac
     1261 tcgcaggaca ataagccatg tttgaaatcc accctgttaa gaaagtctcg gtggttattc
     1321 ccgtttataa cgagcaggaa agcttaccgg aattaatcag gcgcaccacc acagcctgtg
     1381 aatcgttggg gaaagagtat gagatcctgc tgattgatga cggcagtagc gataattccg
     1441 cgcatatact ggtcgaagcc tcacaagcgg agaacagcca tattgtatct attttgctta
     1501 accgcaatta cgggcaacat tcagcgatta tggcgggatt cagtcacgtt actggcgact
     1561 taattattac ccttgatgcc gatctccaga atccgccaga agaaatcccc cgcctggtgg
     1621 caaaagccga tgaaggttac gacgtggtag ggactgtacg ccagaaccgc caggacagct
     1681 ggtttcgtaa aacagcttcg aagatgatta accggcttat tcagcgcacc accggcaaag
     1741 cgatgggtga ctacggttgt atgctgcgcg cctatcgccg tcatattgtc gatgcgatgt
     1801 tgcactgcca tgaacgcagc acctttatcc cgattctggc gaatatcttc gcccgccgtg
     1861 ccattgaaat tccagtgcat catgccgagc gtgagtatgg tgaatccaaa tacagcttta
     1921 tgcgcctgat taatttgatg tacgacctgg tgacctgcct taccacaacg ccgctacgta
     1981 tgctgagcct gctcggcagc attattgcga ttggaggttt tagcattgcg gtgctgctgg
     2041 tgattttacg cctgaccttc ggaccacaat gggcggcaga aggcgtcttt atgctatttg
     2101 ccgtgctgtt tacttttatt ggcgctcagt ttatcggcat gggattactc ggtgaatata
     2161 tcggcaggat ctacaccgat gtccgcgccc gcccccgcta ttttgttcag caagttatcc
     2221 gtccatccag caaggaaaat gaataatgaa aaccgtcgtt tttgcctacc acgatatggg
     2281 atgcctcggt attgaagccc tgctggctgc cggttacgaa attagcgcca tttttaccca
     2341 taccgataat cccggtgaaa aagcctttta tggttcggtg gctcgtctgg cggcggaaag
     2401 aggcattccg gtttatgcgc cggataacgt taatcatccg ctgtgggtgg aacgcattgc
     2461 ccaactctca ccagaggtga ttttctcttt ttattatcgc catcttattt gcgacgagat
     2521 tttgcagctc gctcccgcag gtgcatttaa tctgcacggt tcgctgttac caaaatatcg
     2581 tggtcgcgcg ccgctgaact gggtgctggt gaacggtgaa acagaaactg gcgttacatt
     2641 gcaccgaatg gtaaaacgtg ccgatgccgg ggccattgtg gcgcaactgc gcgttgccat
     2701 tgcgccagac gatatcgcta ttacgctgca tcataaattg tgccatgccg cgcgccagct
     2761 actggaacag acattacccg ccattaaaca cggtaatatt ctggaaatcg cccagcgcga
     2821 aaacgaagcc acctgttttg gtcgcagaac gccggatgac agtttcctcg aatggcataa
     2881 gccggcatcc gtactgcaca acatggtacg tgccgttgcc gatccgtggc cgggtgcctt
     2941 cagctatgtt ggcaatcaga aattcaccgt ctggtcgtcg cgtgttcatc ctcatgccag
     3001 caaagcacag ccggggagcg tgatttctgt tgcgccactg ctgattgcct gtggcgatgg
     3061 cgcgctggaa atcgtcaccg gacaagcggg cgacggcatt actatgcagg gctcgcaatt
     3121 agcgcagacg ctgggcctgg tgcaaggttc acgcttgaat agccagcctg cctgcaccgc
     3181 ccgacgccgt acccgggtac tcatcctcgg ggtgaatggc tttattggca accatctgac
     3241 agaacgcctg ctgcgcgaag atcattatga agtttacggt ctggatattg gcagcgatgc
     3301 gataagccgt tttctgaatc atccgcattt tcactttgtc gaaggcgata tcagtattca
     3361 ttccgaatgg attgagtatc acgtcaaaaa atgtgatgtc gtcttgccgc tggtggcgat
     3421 agccacgccg attgaatata cccgcaaccc gctgcgcgta tttgaactcg attttgaaga
     3481 gaatctgcgc attatccgct actgcgtgaa gtaccgtaag cgaatcatct tcccgtcaac
     3541 ttcagaagtt tatgggatgt gtagcgataa atacttcgat gaggaccatt ctaatttaat
     3601 cgtcggcccg gtgaataaac cacgctggat ttattcggta tcaaaacaat tacttgatcg
     3661 ggtgatctgg gcctatggcg aaaaagaggg tttacagttc accctcttcc gcccgtttaa
     3721 ctggatggga ccacgactgg ataaccttaa tgcggcacga atcggcagct cccgcgcgat
     3781 tacgcaactc attctcaatc tggtagaagg ttcaccgatt aagctgattg atggcggaaa
     3841 acaaaaacgc tgctttactg atattcgcga tggtatcgag gcgttatacc gcattatcga
     3901 aaatgcggga aatcgctgcg acggcgaaat tatcaacatt ggcaatcctg agaacgaagc
     3961 gagcattgag gaactgggcg agatgctgct ggcgagcttc gaaaaacatc cgctgcgcca
     4021 tcatttccca ccgtttgcgg gctttcgcgt tgtcgaaagt agcagctact acggcaaagg
     4081 atatcaggac gtagagcatc gtaaaccgag catccgcaat gcccaccact gcctggactg
     4141 ggagccgaaa attgatatgc aggaaaccat cgacgaaacg ctggatttct tcctgcgcac
     4201 cgttgatctt acggataaac catcatgacc aaagtaggct tacgcattga tgtcgatacc
     4261 tttcgtggca cccgtgaagg cgtgccgcgt ctgctggaaa tcttgagtaa gcataatatt
     4321 caggccagca tttttttcag cgtcggcccg gacaatatgg gccgccatct ctggcgactg
     4381 gtgaagccac agtttttgtg gaagatgctg cgctcaaacg cggcatcgct ttatggctgg
     4441 gatattttac tggcaggtac ggcctggcca ggtaaagaga ttggtcatgc caatgccgat
     4501 atcattcgtg aagcggctaa atatcacgaa gtcggcctgc acgcctggga tcaccatgcc
     4561 tggcaagccc gtagcggtaa ctgggatcgg caaacaatga tcgacgatat tgcacgcggc
     4621 cttcgcactc tggaagagat tatcggtcaa ccggtaacct gttctgccgc tgcgggctgg
     4681 cgtgccgacc agcaggtgat cgaagcaaaa gaagcgttcc atttgcgcta caacagcgat
     4741 tgtcgtgggg ccatgccgtt ccgtccactg ctcgaatcag gaaaccctgg cactgcgcaa
     4801 attccggtga ccttacccac ctgggatgaa gtgattggtc gggatgtgaa agcagaagat
     4861 tttaacggtt ggttactcaa ccgcatcctg cgagataaag gcacgccggt ttataccatt
     4921 catgcagaag ttgaaggctg cgcttatcag cataattttg tggatctcct caaacgcgca
     4981 gctcaggaag gcgtgacatt ttgcccttta agcgaactgt tatcagagac gttgccgctc
     5041 ggacaagttg ttcgcggaaa tattgccgga cgtgaaggct ggctgggttg ccaacaaatt
     5101 gcgggtagtc gctgatgaaa tcggtacgtt accttatcgg cctcttcgcg tttattgcct
     5161 gctattacct gttaccgatc agcacgcgtc tgctatggca acccgatgaa acgcgttatg
     5221 cggaaatcag tcgagaaatg ctggcatccg gcgactggat tgtgccccat ctgttagggc
     5281 tacgttattt tgaaaaaccc attgccggat actggattaa cagcattggg caatggctat
     5341 ttggcgcgaa taactttggt gtgcgggcag gcgttatctt tgcgaccctg ttaactgccg
     5401 cgctggtgac ctggtttact ctgcgcttat ggcgcgataa acgtctggct ctactcgcca
     5461 cagtaattta tctctcattg tttattgtct atgccatcgg cacttatgcc gtgctcgatc
     5521 cgtttattgc cttctggctg gtggcgggaa tgtgcagctt ctggctggca atgcaggcac
     5581 agacgtggaa aggcaaaagc gcaggatttt tactgctggg aatcacctgc ggcatggggg
     5641 tgatgaccaa aggttttctc gcccttgccg tgccggtatt aagcgtgctg ccatgggtag
     5701 cgacgcaaaa acgctggaaa gatctcttta tttacggctg gctggcggtc atcagttgcg
     5761 tactgacggt tctcccctgg ggactgacga tagcgcagcg ggagcctgac ttctggcact
     5821 attttttctg ggttgagcat attcaacgct ttgcactgga tgatgcccaa catagagctc
     5881 cgttctggta ctacgtgccg gtcatcattg ccggtagtct gccgtggctg ggattactcc
     5941 ccggtgcact atacacaggc tggaaaaacc gcaagcattc cgcaaccgtc tatttgttga
     6001 gctggacgat aatgccgctg ctgtttttct ccgttgctaa gggtaaattg cccacctata
     6061 ttctttcctg ctttgcacct ttggcaatgc tgatggcgca ttacgctttg ctggcagcaa
     6121 aaaataatcc tctggcgctg cggattaatg gctggattaa catcgctttt ggcgtcactg
     6181 gcattattgc cacatttgtg gtctccccct ggggaccaat gaacacgccg gtgtggcaaa
     6241 ccttcgagag ctataaagtc ttttgtgcct ggtcgatttt ttcgctatgg gcatttttcg
     6301 gctggtacac cttaacaaac gtcgaaaaga cctggtcttt tgccgcgctt tgcccgctgg
     6361 ggctggcgtt gctggtagga ttttcaattc ctgacagagt catggaagga aaacatccgc
     6421 aattttttgt cgagatgaca caagaatcac ttcagccaag ccgctatatt cttactgata
     6481 gcgtcggtgt tgccgcaggt ctggcatgga gtctgcaacg cgatgacatc atcatgtatc
     6541 gccagacagg tgagttgaaa tacggcctta attatccgga tgcgaaaggg agatttgtca
     6601 gcggtgatga gttcgcaaac tggcttaatc aacatcgtca ggaggggatt attacactcg
     6661 tgctttcggt tgaccgcgat gaagatatca acagtctcgc cattccgccc gcagatgcca
     6721 tcgatcgtca ggagcgtctg gtgctgattc agtatcgtcc caaatgatct ggctaacatt
     6781 agtctttgcc agcttgctta gcgttgccgg gcagttgtgt cagaaacagg caacctgctt
     6841 tgtggcgata agcaaacggc gcaaacatat cgtgctgtgg ctgggactgg cgctggcttg
     6901 tcttggtctt gccatggtgc tctggctgct ggtcttgcag aacgtaccgg taggtattgc
     6961 ctacccgatg ttaagtctga attttgtctg ggtgacgctg gctgcagtaa aactgtggca
     7021 cgaaccggta tcgccgcgtc actggtgtgg ggtggcgttc attattggtg gcattgtgat
     7081 cctcgggagt acggtgtaat gggcctgata tggggattat tcagcgtcat tattgcctca
     7141 gttgcgcaat taagcctggg ttttgcggcg agtcatctgc cgccgatgac gcacttatgg
     7201 gattttatcg cggcgctgtt agcttttggg ctggatgccc ggatactgtt actcgggctg
     7261 ctgggttatc tgctttccgt tttttgttgg tataaaacac tacataaact tgcgctaagc
     7321 aaagcctatg ccttgctcag tatgagttat gtgctggtat ggattgcttc aatggtactt
     7381 cccggctggg aagggacttt ttcgctgaaa gcactactgg gagtagcctg tattatgagc
     7441 gggttgatgc tgattttcct gcccacgaca aaacaacgtt actgagtttt ccctgccact
     7501 ttacactgcc gttcccactc atcggggcta taacagcttg cagagaggac tttcaccgta
     7561 tgcagctttt cacgattaat acaataaagc gcattctgca aaggcgagag taaatccccc
     7621 actttcacgg cgaagtcact cttcacttcg gcgatcattt ttatcgcgcc gccagcatcg
     7681 cagagcataa gtacatgtct gttatcttgt ttattgcagc acgatttttt gaccagccat
     7741 tccattgtat tatcctgttt gctaagagtt ttcatatcaa tttacagatt aacagataaa
     7801 ctctttatca acggcaatgg aatggcagaa ctgagaagtg aaacggaggg ttaattattg
     7861 ctgacgttgc acccactctt ttagcgcctg acgtgaaatt ttaataccgc cgtttttcag
     7921 ctccggcggc aaagtcagcc aacgtaccgg ttgctgaaaa cgggccagct tatctttcac
     7981 ccattcacta agatcaacgc tctcgtggtc atactccatc accgccaccg gtcgatgacc
     8041 aaactccttg tcggcaacgg ggacgataaa cacctgcaaa accgcaggat gtgcagcaat
     8101 tacgcgctcg acttcttccg tctgaatacc ctctccgcca ctgaagaata gattgtctaa
     8161 acgtccgaca atggtcagct tgccattatg catctcaccg cgatcgcgcg tagcgtacca
     8221 gccttcgtca ttaaccagtg aaaccagttg cccgttacgc cagtaacctt ctgccatact
     8281 ggcagcccgc agccacactt cattattaac gattttcact tcccgacccg gcagcggcga
     8341 accaacgtct gccaggccgt cggcttcttt cgcacacacc gtggaggcaa actcggtcag
     8401 accatagccg caaaagcaac gaatcccctg ctcgcgcgcc tgttccgtca actcgaccgg
     8461 gatagccgcg ccgccaagta acaccgcttt cagggaaacg gaactgcggt taaccagcaa
     8521 acgccagagt tgtgttggca ccagtgaagc gtgagtacag cctgccagca tttgctccaa
     8581 tggctgttta tcacgcaccg tcatccgcgc accagcgtat aaccagcgcc acataattcc
     8641 ctgaccggag acgtgaaaca gcggtaaaga gagcaaccag tcatcgtgat cgccaaacgg
     8701 aatcagcgat aacacccctt cggcactggc aagatgggcc tgataagtat ggacagcggc
     8761 tttcggcaac ccggtagaac ccgaggtcaa cgtcattgag cacagacgcg tcggctgcca
     8821 cgtagcgaca tgtgcgcctt caacccgctg aatgtgcagc gacgttaatg ccggaaacgt
     8881 gttttcccca tccggcacca gcgcaaattg cagcgtcaga ttgggcagca attcttcaag
     8941 caacggttgc ggcagctgag ggttcacggg caacacccgc gccccgcatt gcagtaacgc
     9001 cagccaagcg agcagcgttt gcggcgtatt ccacgcccgc aacatcacgc cgctgtcctc
     9061 aaccacaccc tgcaccgcaa atccggaggc taattcatcg acgcgagcac aaagctcgcg
     9121 ccagttgagt tgctcgtcat taagacgtaa ggcgatggct tctccccgca cttgccgcca
     9181 gtgacgccac ggccagtcag agaagatcat aacaaccgct ccagtgcatc aacttccacg
     9241 acaggcagcg tgctacccgg ccagcgacgt acctgctgcg cctgcattag atccagcgtg
     9301 tccagccctg gaatggtgtc cggcgttaac caggcagcaa tccgcgccag ttgcgttaag
     9361 cctaagctcg attcaatgga agaactgatc actgccgtca gccccagcgc gtgcgccgcc
     9421 tgtacctgct cgcgaacttt ctccagactg cccgtgagcg tgggtttgat aactaccgcg
     9481 cgcacgccct cttcagccac aaaggcaaaa tccggctcgc gcaagctttc atcccaggca
     9541 atggcaatgc cggtttcacg ggcaaacgct cgcgaatcat cgcgggtttt gcacggctct
     9601 tcgagaaacg cgatgcggtg gcgataatcc gggttaacgt atttggcaaa ctgctgacct
     9661 ttcagcggtg tccaggcgcg atttgcgtca agacgcaaat gcagatccgg aattgcctcc
     9721 agcaacagat tcaccaccat gccgtcgcgc accgcttcgt acaatcccac tttgaccttc
     9781 gccactttct cgcctggcat atctgcaagt ttgaggatca gatcgtccgg atcgccatta
     9841 cacagtggtg ccgtacggta attggccgcc tgcgggagcg tatctgccag ctccgccaat
     9901 gcacaactta cgccaaaggc aacggaaggc atctgcggta attcgcaatc gcctgccagc
     9961 cagttattta cccaggcaag cagcacactt tgcgcctctt cccaggtttc ctgactgaag
    10021 cccggcagtg gggagatctc cccccaccct tcgcgctcgc cctcacgcag gcaaacgtac
    10081 agcccgtcgc gggtttttaa ccgcctgtcg cgcagaacca cccccgcgtc catggggatc
    10141 tgccagcggt atacctgcgc gctacgcatt acggattccg tttgaatttg ctgaagtcag
    10201 gctgacgttt ctggttgaag gcgttgcgac cttcctgacc ttcttccgtc atgtagaaca
    10261 gcatggtggc gttgcccgcc agctcctgca gacctgcctg accgtcacag tcggcgttca
    10321 gtgcagcttt caggcagcgc agcgccatcg gactgttttg cagcatttca cggcaccagc
    10381 gaacggtttc tttttccaga tccgccagcg gtaccacggt gttgacaagg cccatatcca
    10441 gcgcctgttt tgcgtcgtac tgacggcaca ggaaccagat ttcacgcgct tttttctgcc
    10501 cgacgatacg agccatgtag gaagcgcccc agccgccgtc gaaggaaccg actttcgggc
    10561 cagtctgacc gaagatggca ttatctgccg cgatagtcag gtcgcacatc atgtgcagaa
    10621 cgtgaccacc gccgatggaa tagccagcca ccatcgcgac aaccggtttc ggacaggtac
    10681 ggatctgacg ctggaagtcc agcacgttca ggtgatgtac gccggaatca tctttatagc
    10741 cgccgtaatc accacgcact ttctggtcac caccggagca gaacgcttta tcgcctgcgc
    10801 cagtcagaat gatcacgccg acgttatcgt cataacgcgc atctgccagt gcctggatca
    10861 tctcttttac cgtcagagga cggaaggcat tgcgcacctg cggacgatta atggtgattt
    10921 ttgcgatacc gtcggtggat ttttcataac gaatgtcctc aaagccttcg gagcagtcgt
    10981 gccattcaac cggtgcgtaa agcattgctt catcaggata aatcatattg tgtcctttag
    11041 tcagaaacgc aagatctgcg ccagacttgc gattacgcca gcgggatttt cccgatgcgc
    11101 gttatgtccg gcgcgaggaa tgacatggca gtcggcagcc agttccgccg ccagggcgcg
    11161 gaatttgctg tcacgttcac cacataaata ataaaacgca aatgtgcggg cgctaaggtt
    11221 agcacgtaaa tcaggctgga cggcgagaga agtcgcctcc agcatggcgg caagggttgc
    11281 gccattattg ttgctgcgca gcaccaccag ctcccggcgt tgatcgtcat tgagcgaggc
    11341 aaaaacaggc tgttgatacc agtcggcaaa taccgcggtt aatggttcgg aacgaaaacg
    11401 ctgcgcccat tggcgatcgg aacgctgacg ttccgcgcgt tgttcagcat tttgcagccc
    11461 cggatgaccg ccttcgacaa caaccccaca aagccccgcc agcccctggc aagcggccat
    11521 catcgccacc cgtccgccaa gcgagtaccc caccagccag aagctaagga tgttgtaact
    11581 aaccagggtt ttacgcagta agtcggtgac atcatcaaat ccatcgacgc taatcgccgc
    11641 cgaaccaccg tgacctggga gatcaacata caaccgtgag tagtcggcaa acgcctcgcc
    11701 gacttcctgc cattcgaggc aatcgccgga aaaaccgtgc aaaaacacca gccagggtaa
    11761 acctggtttg ccgtgttttg cctgcgcgtg caggatcata aatggcttac ctgcgccagc
    11821 agctgctgga gcgtttgcgc gccgtcggtg tcgttaacca ccatttcaat taccgtggtg
    11881 gtcggagtac gccaggcgtc ggcaagtacc gtttcaagtt cctgccagtt ttgcggacga
    11941 tgatatttca gctcaaacat cgcggcagcg tgctcaaaat ggacgttttg cggcatcaga
    12001 tagaaacgct cgcgctcgct tttcggcgtt ggcagcagcg agaaaatttg cccgccgttg
    12061 ttgttcacca caattaatac cagcggcgcg gaaacctggc gcaataacgc cagcgcattc
    12121 aggtcgtaaa gtgcggagag atcgcccaca atcgccagcg tcggtttgcc gcttgcccgc
    12181 tgaacgccgg cggcagtcga gagcagcccg tcgataccgc tggccccacg gttgctgtac
    12241 accgggtaac ctgccggaag ttgcgaaagc gcatcaatca gacgtaccac caggctgtta
    12301 ccgacaaaca attgcccctg ttcaggcaga tagtcgctga tgcgatgcgc cagttgcgct
    12361 tcgccaaacg catcgcgaca ggcaataacc gcctgcattg cctgttccgc caggcgcggg
    12421 atttcaacgc accagggctg gcgtttttct gccggatgca gttccagcca gtcggcaata
    12481 ttggcaatta agcggcgtcc acgatggtgt gccggatcaa gccgcccttc gatgtcatcc
    12541 acaatccagt actcttcggg ttcacagctt gcctgccatt gcaggagccg tttgcccgtc
    12601 aggctgcttc ccagttgcac cacaatttgc gcctgttgca gttcgctggt cgctttggca
    12661 ttgcctaacc agagatcggc acacggcagc ggctgtccgg tttgcgacag cacgtcgcca
    12721 atcagcggcc agccaagagt ttgcgcccac agggcaactt ttttgccctc ttccgcactc
    12781 atgcgcccgg caaccaccac gccgcgcttt tgccgccaga agaaccagtc gcgctgtttt
    12841 tcgctctcaa gacgaggcgc ttcacgcagc cacggcttat catcctgcca ccagtcaccc
    12901 agacgctgtt gccagctaag cccagtgtcg tccatctcgc catacagcgg ttcagcaaac
    12961 gggcagttga tatggacccc accagcatga agcgtaccga gagcatggtc gatggtagaa
    13021 accagccaac gtgcggggat atcctgggtc gggcgcggca gtgaaatact gtgcgtgggg
    13081 tgagaggcga acattcccgg ctgacgaatc gcctgattcg cgccgcagtc aattagctcc
    13141 ggcgggcgat cggcggttaa caggatcagt ttttctccgg ttaacccagc ttcaatcagt
    13201 gccggataga gatttgctac cgccgtgccg gaggtcacaa tcaccgccac cggctgcttg
    13261 ctgactttcg ccagccccag cgccagatgc cccagtccac gctcatcgaa atgggtgtgg
    13321 tgaatgaatg cggaattctc cgccgccgct aacgttaacg gtgtagaacg cgagccaggg
    13381 gcgatgcaga tgtgtcttac gccgtgacgc gttaatgctt ccagaatgac cgccgcccag
    13441 cgtcggttaa atgcgcttac tgacatgagt ttgtccggta tcaatattgc ggctaagtat
    13501 aaggagctac aaaaatagat tattgatatg aatcggtaat gatgcgactc attactattc
    13561 catttgtaat aaagtacgca gccctgccgc tttgttgtcg atttcctgcc actcttgctc
    13621 ggggtcggaa ccacggacaa tgcccgcgcc agcatatagc cgcacgacat tgccgctgat
    13681 ttttgctgag cgcagggaaa cgcagaattc gctttgttgt aatgagagat agcccgcaga
    13741 accggcgtac cattctcggg tgaacggttc gtgatgggcg ataaactgtc gcgccagatc
    13801 gcgcggtaag ccagcaactg ctgccgttgg ctgcaactga tgtaaacaga tcacatcatc
    13861 cgctttgttg agtgaagtcc agatacagcg gcgaagatgc tgcactttac gcagacgcag
    13921 tacctgcggc ggtaaaacat ccagcgtctg ggtatcggcc tgtaatcgtt ggcagatatc
    13981 ttccaccacc agcatgttct cgcgctggtt tttatcatcc gccatcagcc actctcctaa
    14041 ctgctgcgcc tgcttatcat caggatgatt tgctactgtt cccgccagcg cttcagtacg
    14101 cagcgctttg tcacgccgcc gccataaccg ttccggtgaa gagccaagaa aagcattttc
    14161 gccatcaaag gccatgtaaa aatggtagca attgagattc agtcgacgac tggcagccat
    14221 catcgccgcc gcattgaccg gacttgcgaa atgcaggtca gttgcccgag caagcaccac
    14281 tttgtcgagc tcaccttcgg cgatggtttt cgttgccagt tcgattaatt gcgtccagcc
    14341 cgttttgtcc ggccagtgtt gttctcgcgt agtggttaaa tgtaacccag gcaagggctt
    14401 gatactcacc agtgtggcga taaattctct tgccttaatc gcatcgtgct gaagggagct
    14461 ttcgctgaat agcgtcagcc gcagcgtggc tttaccgcca cagcgtcgcc attccaggcg
    14521 tggtaaaagt aaattgccct gcgacgggtc aaacgcattc agcccccaaa tgcgtaagtc
    14581 ggcgtgttcc gggtgctggc gaagaaaacg ttgtgcctgg tccaacgacg taaaacgggt
    14641 aatcgcgccc aggacggcag cttcttcatc accattacgt tgttgccagt agaattgcgg
    14701 gtacgtttgc tgactggcca gccagctcaa ggcatcaaaa gcgtctttga gagggaaagg
    14761 aatatcgata acccgaatgc cgggtgtcgc cggaatctct tgcgacaaat ggcgcagtag
    14821 attttccagc gccgtagtaa gtgattgcac gcggacctct ccctgttaaa agcctcacat
    14881 tatacggggt actacaaaaa aaatgcagta ccccggtgta gggaggtttt aacggcgtgc
    14941 cagcaacagt cctagtacca gcccaacggc cgcacccaca ccaattccct gccagggttt
    15001 ttcgtggacg tagtcatcag cacgataaac agcctgcttc gcccgatagt aataactgtc
    15061 tgaagcctgg ctaacccgtt ttttcacatc atccagcgct ttttctgcac gcgctttcag
    15121 ctcaacatat ttctgatcgg cgggatcgcc agaggagcgg agcacctctt ccagtgtttc
    15181 actaagcagc gtcaggtcgt catcgatacg tgtatcacca aactgattag acattctcgt
    15241 tctccatttg cgtaaaacct gtcactaact atagacaagg gttattgatt acgcctgaat
    15301 tacctcgcgt gccatcccaa tgtgtgggat accatcttct tcatagatct ccgtcacagg
    15361 gataaaaccg aaactctggt agaagttttg cagatgcgcc tgcgccccga ggtagacagg
    15421 cttatcaggc cagtgatgcg tgcaagtgtc gagggtttta ctcatcagtt gctgccccac
    15481 tttttcgcca cgcaacgctt cactgacaat cacccgacca ataacgaccg gctcaagatc
    15541 atcatcactt ttcagaatcc tcgcatatgc caccagttca tcatttttcc aaccgagaat
    15601 atggcgatta tcccccgtca aatcatcacc atcgatatcc tgataagggc aattttgctc
    15661 gacaacaaac accgcgcaac gcagttgtaa taaggcataa agctgggaaa ccgataattc
    15721 agagtgatgc agatcttgcc attcaatcat aatgctctcc ttttagatgt atgccttata
    15781 ctagccggaa tattttttga aacgtgatga actcatggaa ttaatttttt taggtacttc
    15841 ggccggtgtg ccaacccgca cgcgcaatgt cacggcaata ttgctaaacc tgcaacatcc
    15901 gacccagagc ggactttggt tgtttgactg cggtgaaggc acccagcatc agctactgca
    15961 taccgccttt aaccctggaa aactggacaa gatttttatc agtcaccttc atggcgatca
    16021 tctttttggt ttacccggct tgctgtgcag tcgttctatg tcaggcatta tccaaccctt
    16081 aacgatttat ggtccgcaag gtatccgtga atttgtggag accgcgctgc ggattagcgg
    16141 ctcatggacc gattatccgc tggaaattgt cgaaattggc gctggcgaaa ttctcgatga
    16201 tggcctgcgc aaagtgaccg cttatccaat ggaacaccca ctggaatgct atggctatcg
    16261 tattgaagaa catgataaac cgggtgcatt aaatgcccag gcattaaaag ctgctggcgt
    16321 accgcctagc ccactgtttc aggaattaaa agcgggcaaa acaatcatgc tggaagatgg
    16381 aaggcagatt aacggcgcag attacttagc tgctccagtg ccaggtaaag cgctcgctat
    16441 tttcggcgat accggcccct gcgatgccgc acttgacctg gctaaaggtg tcgatgtcat
    16501 ggtgcacgaa gcgacgctgg atataaccat ggaagccaaa gccaatagtc gcggtcatag
    16561 ctctacacgc caggctgcga cactggcccg tgaggctgga gtcggtaagc taatcattac
    16621 ccacgtcagc tcgcgctatg atgacaaagg ttgtcagcac ctgttacgtg aatgcaggtc
    16681 aattttcccg gcgactgaac tagcgaatga tttcaccgtg tttaacgttt aactctgctt
    16741 attattaaaa acagggcgaa acttgccctg ttatcgcaac ccgcgctaca tatatccact
    16801 cacacgggca tattgagcga tttttgatat ttattcatct ccttacgcac tatattcaga
    16861 gcaataagtt gcctcttttt atcttcattc agcacaacct ttgcaactaa aaggagcata
    16921 tgatggttac agttgtcagc aattattgtc aattatctca aacccaactc agtcagacat
    16981 ttgcagaaaa atttactgtg accgaggaat tactgcagtc tttaaaaaaa acagcgttat
    17041 ccggagatga agaaagcatt gagttactgc ataatattgc gttaggttat gatgaatttg
    17101 ggaaaaaagc tgaagatatt ctttaccata ttgtta
//
LOCUS       DBFIFI010000088        16604 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.088, whole genome shotgun sequence.
ACCESSION   DBFIFI010000088 DBFIFI010000000
VERSION     DBFIFI010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16604
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.088"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..631
                     /locus_tag="N7B39_002746"
     CDS             <1..631
                     /locus_tag="N7B39_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
                     /protein_id="HGU2422700.1"
                     /translation="DQEQAYEVQLALIDNGVVDEEMLNNAIDLLVDLPAEYRYRMRNQ
                     LFDNKELPSGIINKLDQQYRFNSDWALAVVSMKNSTRRQSERGLHRWNHEDSDIFAEL
                     ATIKDKSDDEWWRALLQSRNDHLRQTALRNAHTPASLLTTLTESQDRSLAINNPQLAA
                     DVKTVWLKEDPSLLLFVDKPDLSQLRDLVKTGATRKIRNEARHRLEEKQ"
     gene            628..1077
                     /locus_tag="N7B39_002747"
     CDS             628..1077
                     /locus_tag="N7B39_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2422701.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNGGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKNYCPP"
     gene            complement(1162..2073)
                     /gene="rdgC"
                     /locus_tag="N7B39_002748"
     CDS             complement(1162..2073)
                     /gene="rdgC"
                     /locus_tag="N7B39_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HGU2422702.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMANPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            2198..3106
                     /gene="mak"
                     /locus_tag="N7B39_002749"
     CDS             2198..3106
                     /gene="mak"
                     /locus_tag="N7B39_002749"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HGU2422703.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            complement(3249..4433)
                     /gene="araJ"
                     /locus_tag="N7B39_002750"
     CDS             complement(3249..4433)
                     /gene="araJ"
                     /locus_tag="N7B39_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HGU2422704.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(4559..7702)
                     /gene="sbcC"
                     /locus_tag="N7B39_002751"
     CDS             complement(4559..7702)
                     /gene="sbcC"
                     /locus_tag="N7B39_002751"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HGU2422705.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIEEVNTRLQNTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLMLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLLQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            complement(7699..8901)
                     /gene="sbcD"
                     /locus_tag="N7B39_002752"
     CDS             complement(7699..8901)
                     /gene="sbcD"
                     /locus_tag="N7B39_002752"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HGU2422706.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            9091..9780
                     /gene="phoB"
                     /locus_tag="N7B39_002753"
     CDS             9091..9780
                     /gene="phoB"
                     /locus_tag="N7B39_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HGU2422707.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            9838..11133
                     /gene="phoR"
                     /locus_tag="N7B39_002754"
     CDS             9838..11133
                     /gene="phoR"
                     /locus_tag="N7B39_002754"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HGU2422708.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSVISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIVKNSD"
     gene            11540..12859
                     /gene="brnQ"
                     /locus_tag="N7B39_002755"
     CDS             11540..12859
                     /gene="brnQ"
                     /locus_tag="N7B39_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HGU2422709.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            12935..14308
                     /gene="proY"
                     /locus_tag="N7B39_002756"
     CDS             12935..14308
                     /gene="proY"
                     /locus_tag="N7B39_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HGU2422710.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            14464..16281
                     /gene="malZ"
                     /locus_tag="N7B39_002757"
     CDS             14464..16281
                     /gene="malZ"
                     /locus_tag="N7B39_002757"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HGU2422711.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNLQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGREVARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     MALFALYQRMIALRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPFLNVVQWQRKEGHGQLTDGILALPAISATVWMN"
     gene            16469..>16604
                     /locus_tag="N7B39_002758"
     CDS             16469..>16604
                     /locus_tag="N7B39_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2422712.1"
                     /translation="MTISNSNLLKPFITGTELPPFKEDVFSKTEEQFKKSTYGKSLFI
                     S"
BASE COUNT         3725 a         4178 c         4482 g         4219 t
ORIGIN      
        1 agatcaagag caagcctacg aagtacagct ggcgctgatt gataatggtg ttgttgacga
       61 agagatgtta aataatgcca ttgatttact ggttgattta cccgctgaat accgctatcg
      121 aatgcgtaac caattatttg ataataaaga attaccttcc ggaattatca acaaactcga
      181 tcaacagtat cgcttcaact ctgactgggc gctggctgtt gtcagtatga aaaatagcac
      241 ccgtagacaa agtgaaagag gattacaccg ctggaaccat gaggatagcg atatttttgc
      301 ggaactggcg acgataaaag ataaatcaga tgatgaatgg tggcgtgcat tgctgcaaag
      361 tcgcaatgat catttgcgcc aaacggcatt acgcaacgca cataccccgg cgtcgttgtt
      421 aacgacattg actgagtctc aggatcggtc gctggctatt aataatccac agctggctgc
      481 cgatgtgaaa acggtgtggc taaaagagga tccatcatta cttttatttg tcgataaacc
      541 cgatctttcg cagttacggg atttagtgaa aaccggggca acgcggaaaa ttcgcaatga
      601 agcgcgtcac cggcttgagg aaaaacaatg acacaacgcc cctggagcaa gttacagcgg
      661 gagatctatg acttactgac gccaacgatc aacctgcaaa ttcactgtac ccgttatccc
      721 atgcgcagcc aaaatggtgg aagcaccgat ttaccgcgtt attggatcac gttggataaa
      781 aacgtgatct gggattaccc gaaagacttt attgcaggca acggtggagt gcgtaatttt
      841 cacggtgaaa cttgctggta tccttacctg acggatattt gttcaatttc tgatttattg
      901 cgtgaatata ttgacacccc aaaagcggag ttgttaacaa aacaatttac cagcgataaa
      961 tgggggttag tgaatattct gcgtgctgct gacaggcgaa tagggatgag gcgattagat
     1021 cagttacggc gtaaaacgca taatattgct gcgctaaaaa attattgccc gccgtagcga
     1081 ataattatat gcctggtgtg gcttcgtacg ccggataaga cgcggcaggc gtcgcatccg
     1141 gcattaaagg aaaatcagca attaacgttg tgcttcgcca cctaatcctt caatcaggtt
     1201 ttgaattaac gctgccagtt caccagtcat caggatgaaa tcggcatcaa aacgctgggc
     1261 gaaatcttca cggtcgatat cttcgttttg atcgcgcagc tcgtcgcaga acttcagacg
     1321 cttgagcgaa ccatcgtcgc acatcacaaa ctgaatgcgc tgctgccagt cgagcgccag
     1381 tttagtcacc acttttccgg cttcaatgtg attggtgatc tcttcgctgg tcagatcttg
     1441 tttcttcgcg cggatcactc cgccatcttc cagcaacgat ttcagctcgg cttcatcaag
     1501 cagctggaag ccctgtgccg cactaccgga acgtacccat tcggtcagcg tcagttcaat
     1561 cgggtttgcc atgctcaatg gtacaaccgg taacgacccc aggcttttac gcagcagtgc
     1621 cagcgtatct tccgcttttt tggcactggc gcagtccacc ataatcaaac cgttaaccgt
     1681 gtcgatccac atcattgtct ggctaaaacg gctgaaagca cgcggcaaca gagagtgcag
     1741 cacttcgtct ttcagcgaat ctttttcggt tttcttcagc ttacgcgcct gttccgcttc
     1801 cagtttggcg attttcgctt ccagcgcctg tttaatcacc ggagacggta ggattttttc
     1861 ttctttgcgc gcgcagataa caatttgacc attggcaacg tgcgttaacg catcgctgtg
     1921 cgatcccatc ggaggaaccc agcccatctt cgccatgtcc tggctgccgc atggggtaaa
     1981 tgccatcgag gctagctgtt tttccatctc ttctgcacgc agcgaaatct cgcggctaag
     2041 acggtaaacc attaaatttt tgaaccacag catgataatt tccacggcct tgtcgttaaa
     2101 tttagcgggc atgataacga attgtcggcg gccttgcatt gccaatccgg ttgtccgtct
     2161 ctacgctatt gatattgaaa aaaataagga gagtaccgtg cgtataggta tcgatttagg
     2221 cggcaccaaa actgaagtga ttgcactggg cgatgcaggg gagcagttgt accgccatcg
     2281 tctgcccacg ccgcgtgatg attaccggca gactattgaa acgatcgcca cgttggttga
     2341 tatggcggag caggcgacgg ggcagcgcgg aacggtaggt atgggcattc ctggctcaat
     2401 ttcgccttac accggtgtgg tgaagaatgc caattcaacc tggctcaacg gtcagccatt
     2461 cgataaagac ttaagcgcga ggttgcagcg ggaagtgcgg ctggcaaatg acgccaactg
     2521 tctggcggtt tcagaagcag tagatggcgc ggcagcggga gcgcagacgg tatttgccgt
     2581 gattatcggc acgggatgcg gcgcgggcgt ggcattcaat gggcgggcgc atatcggcgg
     2641 caatggcacg gcaggtgagt ggggacacaa tccgctaccg tggatggacg aagacgaact
     2701 gcgttatcgc gaggaagtcc cttgttattg cggtaaacaa ggttgtattg aaacctttat
     2761 ttcgggcacg ggattcgcga cggattatcg tcgtttgagc ggacatgcgc tgaaaggcag
     2821 tgaaattatc cgcctggttg aagaaagcga tccggtagcg gaactggcat tgcgtcgcta
     2881 cgagctgcgg ctggcaaaat cgctggcaca tgtcgtgaat attctcgatc cggatgtgat
     2941 tgtcctgggg ggcgggatga gcaatgtaga ccgtttatat caaacggttc cgcagttgat
     3001 taaacaattt gtcttcggcg gcgaatgtga aacgccggtg cgtaaggcga agcacggtga
     3061 ttccagcggc gtacgcggcg ctgcgtggtt atggccacaa gagtaaaaaa cccaggcaat
     3121 tggcgcatca tgcctgatgc gacgcttgcc gcgtcttatc aggcctacaa aatgtgccag
     3181 aaccgcgtag ggcggataag gcgttcacgc cgcatccggc aataagcaac gcacaccgtg
     3241 acagtaatct accccagtgg tttcgccagc accggactat ccgccgcttg ctggcgctta
     3301 tagcgaccat acagcagcaa cgatgacatc gcagcaaacg aaagcagggc ggcaggcagc
     3361 gccacgtaat tatatgccag ccccagcgtc agcatcatac ctccgcaata tgcgccgacg
     3421 gcgctaccga ggttaaacgc tatttgccca cctgcggcac ctaataactc tccgcctttg
     3481 gcattttgca gtaacaatat ttgcagaggt gctgaaaggg caaataatcc cgcgcaacaa
     3541 ataaaagcaa aaataagcga cgttgttttc atgccgccgc agaaaaagag catcagcagt
     3601 gccagtacaa ttataaagtc agtcactgct gcaatgcgca gtggtgaata acgtcctgaa
     3661 atcctgccac ttagcatatt tcccagcacc atccctagcc caactaacat cataataaag
     3721 gtcatcgccg tttccgaaaa accggaaata aacatcatgt atggctttac gtagctgaac
     3781 caggcaaaca cacctgcgtt gccaaacatg gtggcggcga aaattaacca cggggccggg
     3841 ctgcgcaaaa agtgaaattg ttcgcgcagc tttcctttcg cctcgtcgcg aatatccggc
     3901 acccaaaaat agaccgatgc catcaccgca atattaaaaa cagcgatcaa taaaaaggtg
     3961 taacgccagc taaattcctg acttaaatac gttcccagcg gaatgcccag caaattggcg
     4021 actgtcatcc cggaaaccat ccccgccacg gcggcggtga cttttccggg tttgataatt
     4081 tttgataaca cgatcgctcc gacgccaaaa aatgcgccat gcggaaagcc ggaaaccagg
     4141 cgaccaatgg caagcatcag gtacgacgaa gagagcgtga acatggcgtt gccaatgacg
     4201 cacaacgcca ccagaaacaa caagatatgt ttgagtgagt agcggctgga aaagagtgcg
     4261 attattggcg caccgaccac cacccccagc gcataatacg agatcatatg cccggcagca
     4321 ggaatcgaaa ttcctacgtt atgcgccagc tccgtgagca cgcccataat gccaaattcg
     4381 gccatcccca aaccaaacgt gcccagagcc aaagataaaa tgactttttt cataccacca
     4441 tcacaactac cagaacggct accaatcacc gtagccaata aagtgatagc ttgcaggata
     4501 acgaccagca aggccaagag ataattcgcc cctctgtatt cattatcctg ctgaatagtt
     4561 atttcactgc aaacgtactt tccagtttgc tgtagcccag gccgttgatc ttcttcactt
     4621 tgatctgcac aggaatacgc tctttcatcg cttcaacgtg gctaatcaca ccgatggttt
     4681 tgccactggc gttcagggca tccagcgcat caagggcggt atccagcgtt tcgctatcca
     4741 gcgtgccaaa accttcatca aggaacagcg agtcaatacg tgttttatgg ctgaccagat
     4801 ccgaaagcgc cagcgccagc gccagactaa cgaggaaact ttcgccgccg gaaagggtac
     4861 gggtatcgcg taccgcatct gcctgccagg tatcaacaac ctcgacttcc agcgcctcgc
     4921 tggctttgcg ctgtaacaga tagcgcccgt gcagccgggt aagttgctga ttagcgagat
     4981 ggactaaatt atccagcgtc agcccctggg caaacttgcg gaatttatcg ccctctttgg
     5041 aacctattag cgaattcaga tatccccagt cctcaacctg ctgcgtcatt tgagcaattt
     5101 gctgcagtaa ggtttgttgt tgctgacggt tatctgcatc ctgcttcagc tgctggcgaa
     5161 tctcgccttg actcgtggtg ttttcacgca acttttggtg agtttgcgct aactcttgct
     5221 gaatctgctc caccgtcaca gtgagagcca acccgtcagg tcggtgttgt tgatgctgtg
     5281 ccagcgtttc tgctgtctga gtgaccagcg tttgcgcctg acggcgctgg ttttccagat
     5341 tctgcttgag ctgttccagc tgcgttagtg tttgttcatc cattagcgcc gcaaggaacg
     5401 cctgctgatc gtcaaagacg ctggcctgta gcgcggtgtc aaactgcgcc tgggcttttt
     5461 gcagactttg cgccgccaga acatcctgtt gctgtaacgt ctgctgctgg ctgtgtaatg
     5521 cgagacattg ttcatgaacc tgccgccagt tatccagcgc cacagtttct tcgctgtgcg
     5581 ggagatcatc actttgcggc aacgtttcca gaatcggcgt cagctgctga atacggtttt
     5641 gcagcgcggt taattcgttc tggcgttgct gccagctctg cgcttcttgc tgacgtgtcg
     5701 ccaaccagct ctcttcttca tcttcctgtg gcaatgtcag tgcataaccc gccaatgccg
     5761 ttaagagctg ctgctggcgt tgttcaattt gctgttgata ctggataatt tgctgattat
     5821 gcgcggcaat ctgcccttgt aattcatgtc gttggctgag taaccgcagc tggcgttcgt
     5881 gctcatcttg tgcatccagc cacggttgaa tatcgtcctg tggctgcaag gtgatattga
     5941 ggctggccgt gacggcttgc cattgttgag taagtgcttg ctcatcttgt cggaggcttt
     6001 gcgcttcgtt ttcatcgcgc tgaagctgct tcgttaatgc atccagttgc ccgcgcagcg
     6061 ccgcaccttc ttcgccgagc tttttaactt cgttttccag cgccagtaat cgagactgat
     6121 taacgccagg ctccagcgcc tgatacgcct cgaccgccgg atggctggtg gaaccacaaa
     6181 gtggacaagg ctgacccgcc tgtaactgtg cacgttgagc ttccagcgtt ttgatgcgcg
     6241 cttcctgctc gcaaatggtt ttcacatcgg caagttgctg cgtcttttct ttataacgct
     6301 ggcgcatttc gttaagtgcg gcattacgtt gcgtctgttc cagagtgaca ttctggatag
     6361 tgaccattaa ctgcgccaga cgtttttgtt ggggaacaat ctgcccatgc agcgcgacca
     6421 ggcgctgacg cagtgggcgt tgctcagcat gttgcgccag ggcggtagca acttcatcgg
     6481 cggttaacat caacgtgatc gccgcaagcg cattaagttt ttgctcagca tgggttaact
     6541 gttgctgcca ttgccgcaga tgctcgcgat cgctggtttg ttgggagaac tgcgcacgcc
     6601 aacccgccag ttcgttgttc cactgacgga agcggtcgtg ttcctgtaac caggtattca
     6661 ggctttgttg ctgctgctgt aattctgctg actgcttcgc cgcgtggtgg cgaatgctcg
     6721 cgcgaagcgc cattgtgttc tgtaagcgag tatttacttc ttcaatctgc tggcgagtat
     6781 gcgccagcgc cgcgctgtgt tctgcgatgc gttcccagtg cggacgaaga tttcgtgccg
     6841 gttgtgccag actgagcgcc gccagttgag gttgcgcttt ttcttcttcg gctaacgcct
     6901 gttgcaaggc ctgctgacgg cggctggctt cttgctgcaa ttcgtccaga cgcgttaacc
     6961 agtttagtga ttgttgttct tgctgctgcg cggtaagtaa ctgtttttct tcgtcagtaa
     7021 gtacctgcaa actcgctgtc agcgattgca cttgttccgg cgtgagcaac gcgacgccgc
     7081 tggcctgcgc ttgcagcttc tccagctctg tgcgggccga tttgtgctgc tcaaaaacca
     7141 tcgccgagat ttgcccgtag atttcagtgc cggttaactc ctcgagcaat tccgcgcgtt
     7201 ctttgggttt ggcattcagg aaggcagcaa attgcccctg cgaaagcagc atcgaacggg
     7261 tgaagcgccc gtaatccagc ccggttaacg tcgctgtcag ttccagctta tctttcactt
     7321 tgtcggcgag aattttgccg tcggcgcagc gcgccagctc tacgcgtggc acctgcaaat
     7381 taccgtcggg ttggttacgc gcccgattct ggctccagaa cgcgcgatac gcttcacctt
     7441 tcacttcaaa ctccacctcc gccagacatt cagcggtatc gcgggtcatg agatcatttt
     7501 gcgattgtga aacgttagag agacgcggag tttcgtgata cagcgccaga caaatggcgt
     7561 ccagcagggt ggttttcccc gcaccggtcg ggccggtaat ggcaaacagc ccgttgctgg
     7621 cgaacggctc gcgggtgaaa tcaatcttcc attcgccttt taatgagttc aggtttttca
     7681 ggcgcaggct gagaattttc atgcttcgtg ttctccggcg agggtatgca acgtcgtggt
     7741 gaaaagatgc tgcagacgtt gctgctgcga ttcatccagt tcttccagtg ccagacggcg
     7801 attgaacacc tcttcgacgc tgagttcgct gagggtttca cgctgttggc tggctaacac
     7861 acgctcgcgc tgttcacgac tccgacgtac cagcaatact tcaacaggca atgattcggt
     7921 taatgcctgg attttgcgct gaatatcatg cagatactca tcagtagtga tttcgatatc
     7981 cagccagaca ggtggctcct gcgatacatc gcgccactgt tccagctgtg cggtaatcga
     8041 cgccagatcg cctttcagca ctgccatggg ttgcgttacc ggtacgttca ggttttccac
     8101 gctctctaat ttgccgtttg aaaatgtcac cagatggaca tatttactct taccgcattc
     8161 atcaaaactc agtggaatgg gggagccgca atagcgaaca tgttccatgc cgccaataat
     8221 ctgtgcgcgg tgaatatgcc cgagcgcgat gtagtcggct ggtggaaagt tttgtgccgg
     8281 aaacgcgtcc agcgtgccaa tataaatgtc acgcacggcg tcacttttac tggcccccac
     8341 ggtcgttaaa tgtcccgtgg cgatgatggg cagaggctga tcgccgcgca gtttgcaggc
     8401 atcggcatag tgttgttggt aataatcggt aatcgctgcc agtaaatgct gctgtttttc
     8461 aataccgtta agccccgcct ggctggtaat aatgtcacgc ggacgtaaaa acggaatggg
     8521 gcacagcact gcgcctggcg tcccgtcgcg acgaggcaag atttgcggcg catgtccggc
     8581 gctggcgacc acggtagtat tgaggaacgc catgatatcg cgcgattcat tcagcgtggc
     8641 gaccgagtca tggtttcctg ccagtaccac cagatgacag ccagtttgct gtaaattgac
     8701 aacaaaacgg ttgtataacg tgcgggcgta actgggcggc gagccggtat cgaaaacatc
     8761 accggcaaca ataatcgcat ccacctgatg ggtttgtgct gtctccagca gccagtcaag
     8821 aaaagcctga tgttcagctt cgcggctttt actgtagaag ttctggccga gatgccagtc
     8881 tgaggtgtga aggatgcgca taacggttcc ctggcgaaaa agcatgggcg cgattatacc
     8941 caaacagatg tgccatttgc ttttttctgc gccacggaaa tcaataacct gaagatatgt
     9001 gcgacgagct tttcataaat ctgtcataaa tctgacgcat aatgacgtcg cattaatgat
     9061 cgcaacctat ttattacaac agggcaaatc atggcgagac gtattctggt cgtagaagat
     9121 gaagctccaa ttcgcgaaat ggtctgcttc gtgctcgaac aaaatggctt tcagccggtc
     9181 gaagcggaag attatgacag tgctgtgaat caactgaatg aaccctggcc ggatttaatt
     9241 ctccttgact ggatgttacc tggcggctcc ggtatccagt tcatcaaaca cctcaagcgc
     9301 gagtcgatga cccgggatat tccagtggtg atgttgaccg ccagagggga agaagaagat
     9361 cgcgtgcgcg gccttgaaac cggcgcggat gactatatca ccaagccgtt ttcgccgaag
     9421 gagctggtgg cgcgaatcaa agcggtaatg cgccgtattt cgccaatggc ggtggaagag
     9481 gtgattgaga tgcagggatt aagtctcgac ccgacatctc accgagtgat ggcgggcgaa
     9541 gagccgctgg agatggggcc gacagaattt aaactgctgc acttttttat gacgcatcct
     9601 gagcgcgtgt acagccgcga gcagttgtta aaccacgtct ggggaactaa cgtgtatgtg
     9661 gaagaccgca cggtcgatgt ccacattcgt cgcctgcgta aagcactgga gcccggcggg
     9721 catgaccgca tggtgcagac cgtgcgcggt acaggatatc gtttttcaac ccgcttttaa
     9781 cgccttgctc atcggacgca gagcagggct tatgatttct taactggagt atcttacgtg
     9841 ctggaacggc tgtcgtggaa aaggctggtg ctggagctgc tactttgctg cctcccggct
     9901 ttcatcctgg gtgcattttt tggttacctg ccctggtttt tgctggcatc ggtaacagga
     9961 ctgcttatct ggcatttctg gaatttattg cgcctttcat ggtggctgtg ggtggatcgc
    10021 agtatgaccc cgccaccggg gcgtggtagc tgggaaccgc tactatacgg cttacaccag
    10081 atgcagctgc gaaataaaaa acgccgccgt gaactgggca atctgattaa acgctttcgt
    10141 agcggcgcgg agtcgctgcc cgacgcggtg gtgctgacca cggaagaggg cggtattttc
    10201 tggtgtaacg gtctggcgca acagattctt ggtttgcgct ggccggaaga taacgggcag
    10261 aacatcctta acctgctgcg ttacccggag tttacgcaat atttgaaaac gcgtgatttt
    10321 tctcgcccgc tcaatctggt gctcaacacc gggcggcatc tggaaattcg cgtcatgcct
    10381 tatacccaca aacagttgct gatggtggcg cgtgatgtca cgcaaatgca tcaactggaa
    10441 ggggcgcggc gtaacttttt tgccaacgtg agccatgagt tacgtacgcc attgaccgtg
    10501 ttacagggtt acctggagat gatggatgag cagccgctgg aaggcgcggt acgcgaaaaa
    10561 gcgttgcaca ccatgcgcga gcagacccag cggatggaag ggctggtgaa gcagttgcta
    10621 acgctgtcga aaattgaagc cgcaccgacg caattgctca atgaaaaggt tgatgtgccg
    10681 atgatgctgc gcgttgttga gcgcgaggct cagactctga gtcagaaaaa acagacattt
    10741 accttcgaga tagataacgg cctcaaggtg tctggcaacg aagatcagct acgcagtgtg
    10801 atttcgaacc tggtctataa cgccgtgaat catacgccgg aaggcacgca tatcaccgta
    10861 cgctggcagc gagtgccgca cggtgccgaa tttagcgttg aagataacgg accgggcatt
    10921 gcaccggagc atattccgcg cctgaccgag cgtttttatc gcgttgataa agcgcgttcc
    10981 cggcaaaccg gcggtagcgg attagggtta gcgatcgtga aacatgcggt gaatcatcac
    11041 gaaagtcgcc tgaatattga gagtacagta ggaaaaggaa cacgtttcag ttttgttatc
    11101 ccggaacgtt taattgtcaa aaacagcgat taatccgcct ttgtcatctt ttattgccat
    11161 aagccagccg atgctggctt attttctttg cagtcaaaat acgggcgtta gattttacaa
    11221 cggttggtga ttttttgttc gcatgattag ccatgtcttt ttcacggaaa tagtgtttta
    11281 tactggttgg tgatttctta tcgctatata cctctggttt ttagatccct ccttgcttta
    11341 aaacgttata agcgtttaaa ttgcgcttca ggtgctgtca tactgactgc attaacgcgg
    11401 taaatcgaaa aactattctt cgccgcgcct ggttgggagt atttcccgct aaaattgttt
    11461 aaatataccg ctgtatcatc cccagggatt ggcacaaaaa tttaacgtta caacaccaca
    11521 tccacaggca gtatgattta tgacccatca attaagatcg cgcgatatca tcgctctggg
    11581 ctttatgaca tttgcgttgt tcgtcggcgc aggtaacatt attttccctc caatggtcgg
    11641 cttacaggca ggcgaacacg tctggactgc ggcattcggc ttcctcatta ctgccgttgg
    11701 cctgccggta ttaacggtag tggcgctggc aaaagttggc ggcggtgttg acagcctcag
    11761 cacgccaatc ggtaaagtcg ctggcgtact gctggcaacg gtttgttacc tggcggtggg
    11821 gccgcttttc gctacgccgc gtacagctac cgtttctttt gaagtgggga ttgcgccgct
    11881 gacgggtgat tccgcgctgc cgctgtttat ctacagcctg gtctatttcg ctatcgttat
    11941 tctggtttcg ctctatccgg gcaagctgct ggataccgtg ggcaacttcc ttgcgccgct
    12001 gaaaattatc gcgctggtca tcctgtctgt tgccgcgatt gtctggccgg cgggttctat
    12061 cagcacggcg actgaggctt atcaaaacgc tgcgttttct aacggcttcg tcaacggcta
    12121 tctgaccatg gatacgctgg gcgcaatggt gtttggtatc gttattgtta acgcggcgcg
    12181 ttctcgtggc gttaccgaag cgcgcctact gacccgttat accgtctggg ctggcctgat
    12241 ggcgggtgtt ggtctgactc tgctgtacct ggcgctgttc cgtctgggtt cagacagcgc
    12301 gtcgctggtc gatcagtctg caaacggcgc tgctattctg catgcttacg ttcagcacac
    12361 ctttggcggc ggcggtagct tcctgctggc ggcgttaatc ttcatcgcct gcctggtaac
    12421 ggcagttggc ctgacctgtg cttgtgcaga attctttgcc cagtacgtac cgctctctta
    12481 tcgtacgctg gtgtttatcc tcggcggctt ctcgatggtg gtttctaacc tcggcttaag
    12541 ccagctgatc cagatctccg taccggtgct gaccgctatt tatccgccgt gtatcgcact
    12601 ggttgtatta agttttacac gctcatggtg gcataattcg tcccgcgtga ttgctccgcc
    12661 gatgtttatc agcctgcttt ttggtattct cgacgggatc aaagcatctg cattcagcga
    12721 tatcttaccg tcctgggcgc agcgtttacc gctggccgaa caaggtctgg cgtggttaat
    12781 gccaacagtg gtgatggtgg ttctggccat tatctgggat cgcgcggcag gtcgtcaggt
    12841 gacctccagc gctcactaaa tcactgaaca tttgttttaa ccacggggct gcgatgcccc
    12901 gtggtttttt attgtgttga tgggttagga attgatggaa agtaagaaca agctaaagcg
    12961 tgggctaagt acccgccaca tacgctttat ggcactgggt tcagcaattg gcaccgggct
    13021 gttttacggt tcggcagacg ccatcaaaat ggccggtccg agcgtgttgt tggcctatat
    13081 tatcggtggt atcgcggcgt atatcattat gcgtgcgctg ggggaaatgt cggtacataa
    13141 cccggccgcc agctctttct cgcgttatgc gcaggaaaac ctcggcccgc tggcaggtta
    13201 cattaccggc tggacctact gctttgaaat ccttattgtc gccatcgccg atgtgaccgc
    13261 ttttggtatc tatatgggtg tctggttccc gacggtgccg cactggattt gggtactgag
    13321 cgtggtgctg atcatttgcg ccgtaaacct gatgagcgtg aaggtattcg gtgagctgga
    13381 attctggttc tcgttcttta aagtcgccac catcatcatc atgattgtcg ccggtttcgg
    13441 catcatcatc tgggggattg gcaacggcgg gcaaccgacc ggtattcata acctgtggag
    13501 caacggcggc ttcttcagta acggctggct tggcatggta atgtcgttgc aaatggtgat
    13561 gtttgcttac ggtgggatcg aaattatcgg gattaccgcc ggtgaagcga aagatcctga
    13621 gaaatcgata ccgcgtgcga ttaactccgt gccgatgcgt attctggtgt tctacgtcgg
    13681 tacgctgttc gtcattatgt ctatctaccc gtggaatcag gttggcactg ccggtagccc
    13741 gttcgtgctg acgttccagc atatgggcat tacctttgcc gccagcattc ttaactttgt
    13801 tgtgctgact gcttcgctgt cggcaattaa cagtgatgta tttggcgtag gccgtatgct
    13861 ccacggtatg gcagagcagg gcagcgcgcc gaaaattttc agcaaaacgt cgcgtcgcgg
    13921 tattccgtgg gttacggtgc tggtgatgac taccgcgctg ctgtttgcgg tgtatctgaa
    13981 ctacatcatg ccggaaaacg tcttcctggt gattgcttcg ctggcaacct tcgccacggt
    14041 gtgggtgtgg attatgatcc tgctgtcgca aattgccttc cgtcgccgtt tgccgccaga
    14101 agaagttaag gcgctgaaat ttaaagtgcc gggtggggta gcaacgacca tcggcgggct
    14161 gattttcctg ctctttatta tcgggttgat tggttatcac ccggatacgc gtatctcgct
    14221 gtatgtcggt ttcgcgtgga ttgttgtgct gttgattggc tggatgttta agcgtcgcca
    14281 cgatcgtcag ctggctgaaa accaataata cttgcgttct gtaagccggt tcaggaggaa
    14341 atcctgatcc ggctattccg aaagttatcc gcccccgtcc tcctccccca aatatccttc
    14401 agatgatgag tgatcctgca ttaggctatg gcaaggtgat cagattttca tcacagggga
    14461 attatgatgt taaatgcatg gcacctgccg gtgcccccat ttgttaaaca aagcaaagat
    14521 caactgctca ttacactgtg gctgacgggc gaagacccac cgcagcgcat tatgctgcgt
    14581 acagaacacg ataacgaaga aatgtcagta ccaatgcata agcagcgcag tcagccgcag
    14641 ccaggcgtca ccgcatggcg tgcggcgatt gatctctcca gcggacaacc ccggcggcgt
    14701 tacagtttca aactgctgtg gcacgatcgc cagcgttggt ttacaccgca gggcttcagc
    14761 cgaatgccgc cggcacgact ggagcagttt gccgtcgatg taccggatat cggcccacaa
    14821 tgggctgcgg atcagatttt ttatcagatc ttccctgatc gttttgcgcg tagtcttcct
    14881 cgtgaagctg aacaggatca tgtctattac catcatgcag ccggacaaga gatcatcttg
    14941 cgtgactggg atgaaccggt cacggcgcag gcgggcggat caacgttcta tggcggcgat
    15001 ctggacggga taagcgaaaa actgccgtat ctgaaaaagc ttggcgtgac ggcgctgtat
    15061 ctcaatccgg tgtttaaagc tcccagcgta cataaatacg ataccgagga ttatcgccat
    15121 gtcgatccgc agtttggcgg tgatggggcg ttgctgcgct tacgccacaa tacgcagcag
    15181 ctgggaatgc ggctggtgct ggatggcgtg tttaaccaca gtggcgattc ccatgcctgg
    15241 tttgacaggc acaatcgtgg cacgggggga gcttgtcaca accccgaatc gccctggcgc
    15301 gactggtact cgtttagtga tgatggcacg gcgctcgact ggcttggcta tgccagcttg
    15361 ccgaagctgg attatcagtc ggaaagtctg gtgaatgaaa tttatcgcgg ggaagacagt
    15421 attgtccgcc actggctgaa agcgccgtgg aatatggacg gctggcggct ggatgtggtg
    15481 catatgctgg gggaagcggg tggggcgcgc aataatttgc agcacgttgc tgggatcacc
    15541 gaagcggcga aagaaaccca gccggaagcg tatatagttg gagagcattt tggcgatgca
    15601 cggcaatggt tacaggccga tgtggaagat gccgccatga actatcgcgg cttcacattc
    15661 ccgttgtggg gatttcttgc caataccgac atctcttacg atccgcagca aattgacgct
    15721 caaacctgta tggcctggat ggataattac cgcgcagggc tttctcatca acagcaatta
    15781 cgtatgttta atcagctcga cagccacgat actgcgcgat ttaaaacgct gctcggtcgg
    15841 gaggttgcgc gcctgcccct ggcagtggtc tggctgttca cctggcctgg tgtaccgtgc
    15901 atttattacg gtgatgaagt gggactggat ggcaaaaatg atccgttttg ccgtaagccg
    15961 ttcccctggc aggtggaaaa acaggatatg gcgttattcg cgttgtacca gcgaatgatt
    16021 gcgctgcgta agaaaagtca ggcgctgcgt cgtggcggct gtcaggtgct gtatgcggaa
    16081 gataacgtgg tggtatttgt ccgcgtgctg aatcagcagc gtgtactggt ggcaattaac
    16141 cgtggcgaag cctgtgaagt ggtgctaccc gcgtcaccgt ttcttaatgt cgtgcaatgg
    16201 caacgcaaag aaggccatgg gcaactgaca gacgggattc tggctttgcc tgccatttcg
    16261 gctacggtgt ggatgaacta aagcatctgt tttatttgtt acgtcgttag tcaccagccc
    16321 tgacactacg cagcgcaaaa aagctgctca atgtcagggc tgaatttgtt aaatatttgt
    16381 cccgatcgat atgagcgatt tttggtattg tttgcccatg atgttatgta caacatgctc
    16441 caggataacc ctgtagtgga gcaaagatat gacaatttca aattcgaatt tattgaaacc
    16501 gtttattact ggtactgagt tgccaccttt taaagaggat gtcttttcta aaacagaaga
    16561 acaatttaaa aaaagcacct atggcaaatc gcttttcatt tccg
//
LOCUS       DBFIFI010000089        16535 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.089, whole genome shotgun sequence.
ACCESSION   DBFIFI010000089 DBFIFI010000000
VERSION     DBFIFI010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16535
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.089"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..130
                     /locus_tag="N7B39_002759"
     CDS             <1..130
                     /locus_tag="N7B39_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2422713.1"
                     /translation="AVGIDGNIKSLEYNFSVSTPLKYPATLDIDRVNVNFNFSYQM"
     gene            146..514
                     /gene="acpS"
                     /locus_tag="N7B39_002760"
     CDS             146..514
                     /gene="acpS"
                     /locus_tag="N7B39_002760"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HGU2422714.1"
                     /translation="MRIGTDIVEIARITKAIINGEMAFYERVYTSHERLLINPAEPDY
                     ERASGFWAAKEALVKAIGCGFRDGVRFQDIEIKHDAAKAPYFVISGRLQEIFIGQGLQ
                     NNSLSISHCRTHAIATVIVY"
     gene            528..1289
                     /locus_tag="N7B39_002761"
     CDS             528..1289
                     /locus_tag="N7B39_002761"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HGU2422715.1"
                     /translation="MNMYKYNDLNEKTVLVTGASGDIGLGICEKYLEQNCDVYALYKS
                     NDVQLTALKASHPAGDKLHIVQCDLACPQSVSALCEQIERQAGKIDVLVNNAGIVKDS
                     LFASMSYEDFTQVIETNMFSIFRLTKDALMLLRAAENPAIINVASIAALIPSVGQANY
                     SASKGAILGFTRTLAAEMAPWGVRVNAVAPGMIESKMVKKVSRAVVRAVTSTIPLRRL
                     GKCEEVANTIVFLSSSASSYIVGQTIVIDGGLVMR"
     gene            1293..1841
                     /locus_tag="N7B39_002762"
     CDS             1293..1841
                     /locus_tag="N7B39_002762"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ family
                     protein"
                     /protein_id="HGU2422716.1"
                     /translation="MSKYTIPSKIFLEMGGWRQPLLMVDKIADYKYGENGFVSVVKHV
                     TYNEPYLLGHFPEDPIMPGVIISEIFGQASEYLSFLTDICDIWRERFEEELKSLRDIH
                     ARIHRPEMLEIIRTRRSQVRGVLAAQNLKFKDIAYPGDSIDVVSKLAFSDASGFKHYS
                     VTAYVGKKLISQGTIINFRETK"
     gene            1863..2144
                     /locus_tag="N7B39_002763"
     CDS             1863..2144
                     /locus_tag="N7B39_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309314.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HGU2422717.1"
                     /translation="MSTMNDAIEQRKEVLMTIKTEIIQRLNIQRDEAQIDDDTALFGN
                     GFKLDSVDATEIVVLLDKVFDIKISESDDPSYMRSINSLATFVIQKKND"
     gene            2180..3340
                     /locus_tag="N7B39_002764"
     CDS             2180..3340
                     /locus_tag="N7B39_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HGU2422718.1"
                     /translation="MKSLAEFHLKNNAVMGVYNNRTLPSSYHDAMTEYKAVRENALLV
                     DYSHLSIVSVMGDDAWALINQLVSADVSIIRDEQAIYSLVLNEEGTIRGDVYVLCSID
                     GYYLLSEDISAAELIASMNTILEKAEELDIQSMPEIQDMRENNWGAILLEGPYSWEIM
                     SEIHGFDVIGLPYYEYMNTEEDLLLFRCGKHGEYAYMTIGEQAKLAEQWEKLLTVGEK
                     YLMQTGGLDYQKIVRLENPCWDASLWEGQAVNPVQLQMQWAVQYDKDDFIGKDAVTEL
                     SQEYTGNKLIGMIAQEECEGIEAGDRVLVEGQDVGYVVKALFSPALQRFIALTLLEKD
                     YAWSDISGYEIQTAHGIIPAQSKCMPFIYNLSMLVSPTEHSYIDASKNKSAA"
     gene            3414..5972
                     /locus_tag="N7B39_002765"
     CDS             3414..5972
                     /locus_tag="N7B39_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HGU2422719.1"
                     /translation="MKTNRKRVVITGLGILSSLAENIQDFREALLNKKNGVKDSERFA
                     KWFENARAAEVLHDIEYSDLNEDVIDSLDNAALWAYKVGKDALSQAGLMDSRDTLKET
                     GMIVGVSSAGTEAFLPLFEQRMEDFSLRKALFSGAFSSCCSSVSTLLGLQGGVELVAT
                     ACTASPNAVGMAFDYIQNGKSKTMLAVGTEPIYLPTFAGFYALNVMHPDTCTPFSGQS
                     GMSIGEGAGAIVLEEYEHAVARGATIYGEILSYATSCDAFHETGPDPRASGAVQVMNK
                     AMANAGVTPEQIDYVNAHGTGTEANDRIETLAMKKVFANHDKLLVSSTKSYFGHNIGA
                     AGIVELIACLATLPDNRVLPTLNFSHARPNCDLDYVPNEFRDRKVNIFMKNNYAFGGN
                     NCCMILSMEPASIPVSTYDSKRVAISGVGAVSAIGHTLNQVLENIWAQDHHVELGSVT
                     FPDDTLEEAKELLAVLDESNQFAELFDEEYSFSEATLPETESNFKTFQVSGLEPRKHL
                     RRFDQRKATRGGTFALIALTEALEQAKRKIKRDGDELGMIMGMSRGPQETTYKYLQSL
                     KPDPRKVRTSEFPGSLMNAIPTFCGISEGIKGYTTTLATGENSALGALTYGYEIVRQN
                     LQPQVLVGGADEYFPSMSLYMDAVTQKIHMTAEASDYQIYGKDPQGYVPGEGACMLLL
                     EDPQLAVARGAEVLAEVVGYGKSCSNTYFDASLLEEKSASMALAIERALTHAGISAGD
                     IDLVCGTSNGSDDHSRIEIDAIYNTFAAQNPAVPVVNYNACFGFVASAAGLLNLAVIT
                     DCIKKQAVPAIPNTVEFFDERVNFVRQSMKLALNHVLLVGATEGGNYYAFVIKG"
     gene            5977..7200
                     /locus_tag="N7B39_002766"
     CDS             5977..7200
                     /locus_tag="N7B39_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase"
                     /protein_id="HGU2422720.1"
                     /translation="MDNAAIISGYSVWMPYAEDCSQLIDNLKQGKRVARTPWFTSNEE
                     AIKCGFKGNPSVATLKQVDDSALDLLSQLIDEALEQAMLDKHCLAGRNVRVYLTGIGP
                     RIDGLDYKSFYNYNDVEDINLTQSITNLHASKMSQDTISSHLARKYRLQYLPPNMNCT
                     SNSSLTAVHLATQGIEQGGIDLAIVLNCSKIKTQDIWFLETQSMLDSEQVQPFGENSK
                     GVAFAEGFSALLLESAHHRRARQQSEGVRVQTTYTQISAGRSNDASWLSTNVHKVMQA
                     AMKQAEIALDDLAAILPHGNGSAVSDNAEAKAIAMFAGERQIPVLAYKGQIGYTATGS
                     GVVDLIIGHHSLTHHQLIAPVGNDVIIDSMASLVLTDGSVTNHSKRHLLKVGVGVDGS
                     VIGVVMTNMQAGRAK"
     gene            7197..8150
                     /locus_tag="N7B39_002767"
     CDS             7197..8150
                     /locus_tag="N7B39_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGU2422721.1"
                     /translation="MTEKSIYLSSWALTEPDDIFYFYQPTWLNAWEAHMYERGLYGTR
                     ANGWGLKRFGVENDIYAPERIRNPQYMPYLEAMEQVTDSALQQMPTKERMKLMKSRTA
                     FIYADSWGESGSFEKISSALHIATIDTLPKNLLKKFSITEPTCKIRGEKQAFVQALRM
                     AQDYLAWDVFDNVVICAAYRAIPVLVFSEEDVGKPPKRLFPRRADEVNLSVERVGCFI
                     LSQRESAIRLQCGEYHSGTAPQHATDIDVMAFSERQQMPSRPAAGVIDLVAHYGESGC
                     LTPALSMDYLTRHIQPGGKMRTVIADKQFGYHYFDLEYLEG"
     gene            8161..8868
                     /locus_tag="N7B39_002768"
     CDS             8161..8868
                     /locus_tag="N7B39_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU2422722.1"
                     /translation="MQSTIKSLPAISLSNIYKCYGAGAGKVDALKSINLDINKGEFLA
                     LCGPSGSGKSTLLNILSGIDKPTSGTVIFLNKLLNQLPEEQLAVIRGKHLGFIFQFFN
                     LMPVLNVFDNVYFPLVLNGQFSKKEATERTLHYLDSVGLSGFGDRKPGQLSGGQQQRV
                     AIARALAHEPMVVIADEPTGNLDLATGEAILDLLLTINQQTGTTFVISTHSSELKARA
                     RRVVEIQDGVLIHDSDA"
     gene            8852..9508
                     /locus_tag="N7B39_002769"
     CDS             8852..9508
                     /locus_tag="N7B39_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2422723.1"
                     /translation="MTQMHKGFLLIGVFLYAAFVAPKIYAATPDAVNQVLQQGLPAQP
                     AALATLTEALKKEYEARHNVVALVFYAYGLLRQADGYAATNDFIHASEYAKSGFFWLD
                     EAVDLQEKNQRVRYLRARVDAYLPADSGRCVVTVKDTEHMLADPAIWTTTIRDHILAM
                     RYRALRHCKDTTRANALLAQIKGQNAALAQSLTQDFNVVPEWDSEELTQVLLPLMKGE
                     "
     gene            9509..10819
                     /locus_tag="N7B39_002770"
     CDS             9509..10819
                     /locus_tag="N7B39_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU2422724.1"
                     /translation="MSGEILAVNCVLLLILFFVFYQLLFYSRDVKFAFLNLFRHKRRS
                     FSTITAIVLGGVAIFLYGGFIDYSFWILKEQTIRTNIGHVQIYNQHYFETSNKNKSLI
                     ADYAALKKAILSDPTLAADISTLSGQLEFTGVISHYESETSSYFSALGGEPLPALKLG
                     SFDKIIAGSDLSRVKGDEITLGSGLAKTLNAKYDDWLDVMVVNTAGGQGALSLKMRGI
                     FESGIKDYDDVAMKIPLDTAQRMMGTDGVSKVLILLKEDDTAAFSAKLRQFIERNHLP
                     LVVKDWRDVSLFYQQVEGLLSGIYFFIKLIVALIVIFMIGNSMAMNIVERTREITTLR
                     AIGLKPLHVTRLFLTEGIFIGVIGAVGSLLVGGVLAWIINLYGIAMPPSPGQTIGYTA
                     FIKTSNPELIWVTVVLPILTATGASVLPALRASRLNISDAFKFS"
     gene            10828..11616
                     /locus_tag="N7B39_002771"
     CDS             10828..11616
                     /locus_tag="N7B39_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HGU2422725.1"
                     /translation="MNMAFYGKWFACLWLATSCVQAASTDNKALEIIRRADEIRSPNK
                     PFRYTLTVTEYKAGATQPENKQVLDISMRFMKPQGNEKADARSLVRFIYPPRDKGKIM
                     LSDWYDLWFYTPELRRPMPISRQQRLIGQISNGDVIVTNFEYAYDSTLMGEVTCAEKQ
                     CYKLALVRKSADITWPKVIYYVEKDGDNRPWKAAYYSQDDQLIKEVLYQDFQPVLGKT
                     RPMKITVTDVRHGNNYSVMEYSDVRLESLPEFHFTKEYIQRGAK"
     gene            11613..13001
                     /locus_tag="N7B39_002772"
     CDS             11613..13001
                     /locus_tag="N7B39_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2422726.1"
                     /translation="MKRTLKISSLLCVALPLTVQADCLSGDEVAQNSDVTLYEQVSYV
                     NKQASAWQIAGKNPYTRHNGYQEAGIGINSGCSIIDNTLDLKLNLYGINEYALKPAGK
                     FETDDSRTRALINRLSLVYSASDSVQFEAGKFAAPSGMFFLRSPSDLQTHYYTGFQST
                     RLHDPKMTSAYQASSWGAKMSVDTRDYAFSASVIPKLATIDKRYVTSGNWSANQQGNS
                     DEAYLLSFSDHRFGEHTPTVNVRLGPSPSLALSDSYHYTPQLTLNVDAAYHRSQQWRH
                     LSHRETAQVEEYQFPDSLYETKDESGVELALGGQYTSDNFSVFGVEYYFQSEGYSRAE
                     QRQQRELIDFLNTTTGYAPLDQAFDSYKYLMASEISNTANQGMLQGKHYLNAWASLPL
                     AGESTLQPSLVVNLVDGSTLLGLHYSTPLSAISNQLEAYAGGYSALGSRYSEFALFGD
                     TLGLYLGFKYYL"
     gene            13015..13956
                     /locus_tag="N7B39_002773"
     CDS             13015..13956
                     /locus_tag="N7B39_002773"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HGU2422727.1"
                     /translation="MNNASQPTVLLFAGQGNPVIGMGSDLWDLNASTKHIWDCASDIS
                     GLNLRRLCQKGPMNRLIQTTVQQLAVTAINVTLYTLCRERLENLTVVGSCGHSVGEYS
                     ALYAAGAISLETLFGMIHFRASLMHDESQRNKGAMLAVKGINREQLQTMITESGIALD
                     ISCDNTPRQQVIGGTQAALNEFATLLMAAGYEPVKLGVSGAWHTRLMEDGVQAMRDYL
                     AGLDIASPEHQVLMNVTAKSEVAPSIIKENLSLHLTHTVKWTESLDTFLNMPTSVAFL
                     EISNKPYLGNMFNDFAGVDQQRVMHCRKAFSDAKVFK"
     gene            13953..14939
                     /gene="ycdU"
                     /locus_tag="N7B39_002774"
     CDS             13953..14939
                     /gene="ycdU"
                     /locus_tag="N7B39_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcdU"
                     /protein_id="HGU2422728.1"
                     /translation="MIPDYLTFIRFQDKRSLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQRLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVIEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSRRYAFLGRLFLQEIDLFFSSENALSTFFAKPLWLRLFILLVIEVMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFYCRFLWHNDFMMACDMYFRWGHFNK"
     gene            15306..16508
                     /locus_tag="N7B39_002775"
     CDS             15306..16508
                     /locus_tag="N7B39_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU2422729.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVAIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYTDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARI
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQIVNK"
BASE COUNT         4172 a         3732 c         4374 g         4257 t
ORIGIN      
        1 ggcagttggt attgatggaa acattaaaag tcttgagtat aacttttccg tttctacccc
       61 attaaagtat ccggcgacat tagatattga tcgggtgaat gtaaatttta atttttcgta
      121 ccaaatgtaa aggataacga gactcatgag aatagggaca gatattgttg aaattgccag
      181 aataaccaaa gccattatta atggagagat ggctttttat gagcgtgttt atacttctca
      241 tgagcgctta ctcattaacc ctgcagaacc tgattatgaa cgggcctctg gattctgggc
      301 ggcgaaagaa gcgttagtta aagcgattgg ttgtggtttt cgtgatggcg tacgtttcca
      361 ggatattgaa ataaagcatg atgcagccaa agcaccttat ttcgttattt ccggaagact
      421 acaagaaatt tttatcgggc aggggttgca aaataactcc ctgagtattt cgcattgccg
      481 tacccacgcc attgcgactg tcatcgttta ttaactcaac atatgtgatg aatatgtaca
      541 aatataatga tttaaatgaa aaaaccgtac tggtgacagg agccagtggt gatattggac
      601 tgggtatttg cgaaaaatat ctggagcaga actgcgatgt ttatgcgctc tataaatcca
      661 acgacgtgca attaacggcg ctgaaagcaa gccatcctgc aggcgataaa ctgcatattg
      721 tgcagtgcga tcttgcctgc ccccagagcg tgagcgcatt gtgcgaacag atagagcgcc
      781 aagctggcaa gattgatgtg ctggtaaata acgcgggcat cgttaaagac agcctgtttg
      841 cctcgatgag ttatgaggat ttcacccagg ttatcgaaac caacatgttc agcattttcc
      901 gtctgacgaa agacgcgctg atgctgcttc gtgcggcaga aaatcccgcc attattaacg
      961 ttgcctctat tgcagcgctg atcccaagcg tcggtcaggc gaattacagc gcgtcaaaag
     1021 gggcgatcct aggcttcacc cgcacgctgg cagcagaaat ggcaccctgg ggcgtgcgcg
     1081 tcaacgcggt cgcaccggga atgattgaat cgaaaatggt gaaaaaagta tcccgcgcag
     1141 tggtccgtgc ggtaaccagt accatcccgt tacgccgtct gggtaaatgt gaagaagtgg
     1201 cgaacaccat cgtcttctta agttcctctg cctccagcta cattgtcggc cagaccatcg
     1261 ttatcgatgg cggcctggtc atgcggtgat ttatgtctaa atacacgatt ccttcgaaaa
     1321 tcttcctcga aatgggcggc tggcgtcagc cgctgctgat ggtcgataaa attgcagatt
     1381 ataaatacgg tgaaaatggc tttgtcagtg tcgtgaaaca cgtcacctat aacgaaccgt
     1441 atctactggg gcatttcccg gaagatccga ttatgcccgg ggtgattatt tcggaaatct
     1501 tcggccaggc cagcgagtat ctctctttcc tgaccgacat ttgcgatatc tggcgcgaac
     1561 gttttgagga agagctgaag tcgttacgtg atattcacgc acgcattcac cgccctgaaa
     1621 tgctggagat tatccgtacc cggcgcagcc aggtacgcgg cgtgctggcg gctcagaacc
     1681 tgaaatttaa agatatcgcc tatccgggtg attctattga tgttgtcagc aaattggctt
     1741 tttcggatgc cagtggcttt aaacactatt ccgtgaccgc gtacgtcggt aaaaagctga
     1801 ttagtcaggg caccattatc aatttccgtg aaaccaagta atccaataag agagtaaata
     1861 ttatgtctac catgaatgac gctattgaac agcgtaaaga agttctgatg accattaaaa
     1921 ctgagatcat ccagcgactg aatattcaac gcgatgaagc gcaaattgat gatgatactg
     1981 cgctgtttgg taatggtttt aaactggact ccgtcgatgc aacggaaatt gtcgttctgc
     2041 tggataaagt atttgatatc aagatcagcg aaagtgacga cccgtcctat atgcgcagta
     2101 tcaatagtct ggccaccttc gttattcaaa agaaaaacga ttaactactt attaaagaat
     2161 taattatcag gacacgttta tgaaatctct tgctgaattt cacttgaaaa ataatgcggt
     2221 aatgggggta tataacaacc gtaccctacc ttcttcttat cacgatgcta tgaccgaata
     2281 taaagccgtt cgtgaaaacg cgctgctggt ggactattct cacctctcta ttgttagcgt
     2341 catgggcgat gatgcctggg cgctgatcaa ccagctggtt tccgcagatg tctccatcat
     2401 tcgtgatgag caggcaatct actcactggt gttgaatgaa gagggcacca ttcgcggaga
     2461 cgtctatgtg ctgtgcagca ttgacggtta ttacctgctt tcagaagata tttcggctgc
     2521 cgaattgatc gccagcatga ataccattct cgaaaaagcg gaagaactgg atattcaatc
     2581 gatgcctgaa attcaggata tgcgggaaaa caactggggg gcaatcctgc tcgaaggccc
     2641 atactcctgg gagatcatgt ctgaaattca tggctttgac gtgattggcc tgccttacta
     2701 cgaatacatg aataccgagg aggatctgct gctcttccgc tgtggtaaac acggtgagta
     2761 tgcgtacatg accatcggtg agcaggcgaa actggcggag cagtgggaaa aactgttaac
     2821 cgttggcgag aaatacctga tgcagaccgg cggcctggat tatcaaaaaa tcgtacgcct
     2881 cgaaaatccg tgctgggatg ccagcctctg ggaaggtcag gcggtgaatc cggttcagtt
     2941 gcagatgcag tgggcggtgc agtacgacaa agatgatttt attggcaagg acgcggtgac
     3001 agagctatcc caggagtata ccggtaataa actcatcggc atgatcgcac aggaagaatg
     3061 cgaaggcatt gaggctggcg atcgcgtgct ggtggaaggt caagacgtgg gctatgtcgt
     3121 gaaggcactc ttttccccag cgttgcagcg tttcattgcc ctgaccctgc tggaaaagga
     3181 ttacgcctgg tctgacatca gcggctacga aattcaaact gctcacggaa ttattccggc
     3241 gcaatcaaaa tgtatgccgt ttatttataa cctgagcatg ttggttagcc caactgagca
     3301 cagttatatc gacgcgtcaa aaaataaaag cgcagcgtaa tgaatatcgc cgcgtgacta
     3361 tagcttacgc ggcttaaatt aaccccaatt ggcgggattg ccaggtatta ttcatgaaaa
     3421 cgaacaggaa aagagttgtt attaccgggc tggggatttt gtcctcactg gcggaaaata
     3481 ttcaggattt ccgggaagca ttattaaata aaaaaaatgg cgttaaggat agcgaacgct
     3541 ttgcaaaatg gtttgaaaat gcgcgtgcgg cagaagtgtt gcatgacatt gaatactccg
     3601 atctgaacga agatgttatc gattcactgg ataacgcagc gctctgggcc tacaaagtgg
     3661 gtaaagatgc gctgagtcag gccggcctga tggatagccg tgacaccctc aaagaaacag
     3721 gcatgatcgt cggtgtttcg tccgcgggaa cagaggcatt tttgccgctg ttcgaacagc
     3781 gcatggaaga tttctcgctg cgtaaagcac tcttttccgg cgcattctca tcctgttgtt
     3841 ccagcgtttc aacgctgctg ggcttacagg gtggggttga actggtggcg accgcgtgta
     3901 ccgccagccc gaacgccgtg gggatggcct ttgactacat ccagaatggt aaaagcaaaa
     3961 ccatgctggc agtgggcact gagcccatct atctgccgac ctttgccgga ttctacgcac
     4021 tgaatgtcat gcatccagac acttgtacgc cgttctcggg tcagtccggt atgtcgattg
     4081 gtgaaggcgc tggcgctatc gtgctggaag agtatgagca cgccgtagca cgcggggcga
     4141 ccatttatgg tgagatcctc tcttacgcca cttcctgcga tgctttccat gaaaccgggc
     4201 cagatccgcg tgccagcgga gcggtgcagg tcatgaacaa ggcaatggca aatgcgggcg
     4261 taaccccgga gcaaatcgat tacgtcaacg cgcacggtac gggtaccgaa gccaatgacc
     4321 gtattgaaac gctggcaatg aaaaaggtct tcgctaacca cgataaactg ctggtgagct
     4381 ccaccaaatc ttactttggc cacaacattg gcgcagcggg gattgtggaa ctgattgcct
     4441 gtctggcaac gcttccggat aaccgcgtac tgccgacgct gaatttcagc catgcacgcc
     4501 cgaattgcga tctggactat gtgccgaatg agtttcgcga tcgcaaagtg aacatcttca
     4561 tgaagaataa ctatgccttc ggcggcaaca actgctgcat gatcctgagc atggagcccg
     4621 cgtccattcc ggtcagtacc tatgattcga agcgtgtggc tatctcgggc gtcggtgcag
     4681 tatccgcaat aggccacacg ctcaaccagg ttctggaaaa catctgggcg caggaccacc
     4741 acgttgaact gggtagcgta accttcccgg acgatacact ggaagaagcc aaagagctgc
     4801 tggccgtgct ggatgaatca aaccagtttg ccgagctgtt tgatgaagag tacagcttca
     4861 gcgaagctac gcttccggaa acggaaagca actttaaaac gttccaggtt tccgggctgg
     4921 agccgcgtaa acacctgcgt cgtttcgatc agcgtaaagc gacccgtggc ggcacgtttg
     4981 ccctgatcgc actcaccgaa gcgctggaac aggcgaagcg taaaatcaaa cgcgacggcg
     5041 atgagttggg catgatcatg gggatgtccc gcggcccgca ggaaaccacc tacaaatatc
     5101 tgcaaagtct gaaacccgat ccacgcaaag tacgtacatc ggaatttcct ggctcgctga
     5161 tgaatgcgat cccaacgttc tgcgggatct ctgaagggat caaagggtat accactacgc
     5221 ttgcgacagg ggaaaactcg gcgctgggcg cgctgaccta cggttatgaa atcgtccggc
     5281 agaacctgca accgcaggta ttggttggcg gcgcggatga gtacttcccg tcgatgtcgc
     5341 tgtatatgga tgcggtgaca caaaagatcc acatgacggc tgaggcgagc gactatcaga
     5401 tttacggtaa agatccgcaa ggttatgttc cgggcgaagg ggcttgcatg ctgctgctgg
     5461 aagatccgca gttggctgtg gcgcgtggcg ctgaggttct cgcggaagtg gtgggctacg
     5521 gcaagtcatg cagcaatacc tacttcgacg cctcattgtt ggaagaaaaa tccgcgtcga
     5581 tggcgctggc cattgaacgc gcccttacgc atgcgggcat ctctgctggc gatattgatt
     5641 tggtatgtgg taccagcaac ggtagcgatg accattctcg tatcgaaatt gacgcgattt
     5701 acaacacctt cgccgcgcaa aacccggcgg tgccggtagt gaactataac gcttgctttg
     5761 gttttgtcgc ctctgccgct ggtttgctta acctcgccgt gattactgac tgcattaaga
     5821 agcaggcagt acctgcgatt ccaaatacgg tggaattctt tgatgagcgt gtgaattttg
     5881 tacgccagtc gatgaaactt gcgctgaacc atgtcctgct tgttggcgca acggaaggcg
     5941 gcaactacta cgcgttcgtc attaagggat aagactgtgg acaatgccgc tattatttcg
     6001 ggttacagcg tgtggatgcc gtatgccgaa gactgttcgc agttaattga caatctgaaa
     6061 caggggaaac gcgttgcgcg tacaccctgg tttacctcca acgaggaagc cattaaatgc
     6121 gggtttaagg gtaatccgtc agtggcgacg ttgaaacagg tggacgatag cgcattggac
     6181 ctgctgtctc agttgatcga cgaagccctg gaacaggcaa tgctggataa gcattgcctg
     6241 gccgggcgca acgttcgcgt ttatctgacc ggtattgggc cgcgtatcga cgggctggat
     6301 tacaaatctt tctataatta caacgatgta gaagatatca acttaacgca atccatcaca
     6361 aatctgcatg cctcaaagat gtcgcaggac acgatttcaa gccatcttgc gcgcaaatat
     6421 cgcttgcagt atttgccgcc taacatgaac tgcaccagta actcgtcact gactgcggtg
     6481 catctggcga cgcagggcat tgagcagggc gggattgatc tcgcgattgt gctgaactgt
     6541 tcgaaaatta aaacccagga catctggttc ctggaaacgc aatcgatgct ggacagcgaa
     6601 caagtgcagc cgtttggtga aaacagtaaa ggtgtggcgt tcgcagaagg ttttagcgcg
     6661 ctcctactgg aaagcgctca tcaccgccgg gcgcgtcaac aaagtgaggg cgtaagggtg
     6721 caaacgactt acacccaaat cagcgcgggc cgcagcaacg atgcctcctg gcttagcact
     6781 aacgtgcaca aggtcatgca ggccgcgatg aagcaggctg aaattgcgct ggatgatctt
     6841 gcagccattc ttccacacgg caacggttcg gcagtgagcg ataacgcgga ggccaaagcc
     6901 atcgccatgt tcgcagggga gcgacagatc cccgttctcg cctataaagg gcagatcggc
     6961 tataccgcaa ccggatctgg tgttgtcgat ctgatcattg gccaccattc gttgacgcat
     7021 catcaactga tcgcacctgt tggcaacgac gtcattatcg acagcatggc ttcgctggta
     7081 cttacggacg gcagcgtgac aaaccatagc aaacgccatt tgctgaaggt tggtgtgggt
     7141 gtcgatggtt cagttattgg cgttgtcatg acaaatatgc aagcggggcg cgcgaaatga
     7201 cggaaaagag tatctacctg tcttcctggg cgcttacgga accggacgac attttttatt
     7261 tttatcagcc gacctggctc aacgcctggg aagcacatat gtacgaaagg ggattatacg
     7321 gcacccgtgc taatggctgg ggactgaagc gctttggcgt ggagaacgat atttatgcac
     7381 cagaacggat ccgtaatcca caatatatgc cttaccttga ggcgatggag caggtgaccg
     7441 attctgcatt acaacaaatg ccgactaaag aacgcatgaa gttaatgaaa tcgcgcacgg
     7501 catttattta tgccgattcc tggggcgaat ccggttcgtt tgaaaaaatt tccagtgcgc
     7561 tgcatatcgc cactattgat acgcttccca agaatttact taagaagttt tcgataacgg
     7621 aaccgacttg caagattcgc ggcgaaaaac aggcattcgt tcaggcgctg cgtatggcgc
     7681 aggattatct cgcctgggat gtattcgata acgtggtgat ttgtgctgcc tatcgggcaa
     7741 ttccggtgct ggtattctct gaagaagatg tgggtaaacc gcctaagcgt ttatttcccc
     7801 gtcgtgctga tgaggtgaat ttatctgttg aacgagtggg ttgttttatt ctcagccagc
     7861 gtgagagcgc catcaggctg caatgcgggg aatatcattc cggcactgcg ccacagcatg
     7921 ctacggatat tgatgtcatg gccttcagcg aacggcaaca aatgccgtcc cggcctgctg
     7981 ctggcgttat cgacctggtg gcccattacg gtgaaagcgg ttgtctgacg cccgcgttga
     8041 gtatggatta tttaacccgt catattcagc ccggtggaaa aatgcgtacg gtcattgccg
     8101 ataaacagtt tggttatcac tactttgacc ttgaatacct tgaaggataa cgacagtcgt
     8161 atgcaatcta ctattaaatc gttgcccgct atttcgctta gcaatattta taagtgttat
     8221 ggcgctgggg caggaaaggt tgatgcgctt aaaagcatta atcttgatat caataaaggc
     8281 gaatttctcg cactgtgtgg gccgtcaggc agtggtaaaa gtactctgtt gaatattctc
     8341 tcgggtattg ataaaccgac cagcggaacg gtgatatttt taaataaatt actgaaccaa
     8401 ctgccagaag agcagttggc tgtgattcgt ggaaaacatt tgggttttat ttttcagttt
     8461 tttaacctta tgccggtatt aaatgttttc gataacgttt atttcccact ggtgcttaat
     8521 ggtcagttca gtaaaaaaga agccactgaa cggacgctgc attatctcga tagtgttgga
     8581 ttaagcggtt ttggcgatcg taaacctggg caactatcgg gcggtcaaca acagcgtgtc
     8641 gccatcgcca gggcactggc ccacgaaccg atggtggtca ttgccgatga accgacggga
     8701 aatttagacc tggcaaccgg cgaggctatt ttggacctgt tgttgacgat taatcagcag
     8761 accggcacca catttgttat ttccacgcac tccagcgaat taaaagcgcg ggcacggcga
     8821 gtggttgaaa ttcaggatgg agtattgatt catgactcag atgcataaag gatttttgct
     8881 catcggcgtc tttttgtacg cggcgtttgt ggctccgaag atttacgcgg caacacccga
     8941 cgctgttaac caggttctgc aacaggggct cccggcgcag cctgccgcgc tggcgacgct
     9001 gaccgaggcg ttaaagaaag agtatgaagc caggcacaat gtggtggcgc tggtttttta
     9061 cgcctatggc ttactgcgtc aagcggacgg ttatgcggca acaaacgatt ttatccatgc
     9121 ctcagaatac gccaaaagcg gctttttctg gctggatgaa gcggtggatt tgcaggagaa
     9181 aaatcaacgc gtacggtatt tacgcgcccg cgtagatgcc tatttacccg cagactcagg
     9241 gcgttgcgtg gtgacagtca aagacaccga acacatgctg gctgacccgg cgatatggac
     9301 gacaacgatc cgcgaccata ttctggcgat gcgttatcgc gcattacgtc actgcaagga
     9361 caccacccgc gcgaatgcgt tgttggcgca aattaaaggg caaaacgccg cgcttgcgca
     9421 gtcgctgacg caggatttta atgttgtacc agaatgggat agtgaagagt taacccaggt
     9481 actgctgccg ctgatgaaag gtgagtaaat gtccggggaa attttagctg ttaattgcgt
     9541 tctgctgctg attctgtttt ttgtgtttta tcagcttctg ttttattcac gagatgtcaa
     9601 atttgcattt ttgaatctct ttcgccacaa gcgtcgctct ttttctacca taaccgctat
     9661 tgtattgggt ggcgtggcaa tttttttata tggcggattt attgattact ctttctggat
     9721 cctgaaagag cagacgattc gtaccaatat tggtcatgta caaatttaca atcagcacta
     9781 ttttgaaacg tcgaataaaa ataagagcct gattgccgat tatgcggcgt taaagaaagc
     9841 tattttgtcg gacccgacgc tggcggcgga tatttccacg ctctccgggc aactggagtt
     9901 tactggcgta atttcacatt acgagagcga aacctccagc tatttctccg cgctgggggg
     9961 cgagccgtta cccgctttaa agctgggttc gttcgataag attatcgcgg ggagcgatct
    10021 ttcccgggta aaaggtgatg aaatcaccct gggcagcggt ctggcgaaaa cgttgaatgc
    10081 gaaatatgac gactggctgg atgtgatggt ggtcaacacg gcgggcgggc agggcgcatt
    10141 gtcgctaaaa atgcgtggca tttttgagtc aggcattaaa gattatgacg atgtcgcgat
    10201 gaagatcccg ctggacaccg cgcagcgcat gatgggtacg gatggcgtca gcaaagtgct
    10261 tattctgctt aaagaggatg acaccgcggc gtttagcgca aagctgcgtc aatttatcga
    10321 acgcaatcat ttgccgctgg tggtcaaaga ctggcgtgat gtttcactct tttatcaaca
    10381 ggttgaaggg ttgctatcgg ggatctattt cttcatcaag ctgattgttg cgctgattgt
    10441 catcttcatg atcggcaact cgatggcaat gaacattgtt gagcgcaccc gtgaaattac
    10501 cactctgcgt gccataggcc tgaagccgct tcacgtcacg cgcttgtttc tgacagaagg
    10561 catttttatt ggtgtgattg gcgcagtcgg cagcctgctg gtggggggcg tgttagcctg
    10621 gattattaat ctttatggta ttgcgatgcc gccatcgccg ggacaaacca tcggctatac
    10681 cgcctttata aaaaccagta acccggaatt gatttgggtg acggtggtat tacctatttt
    10741 gacagcaaca ggtgcctctg tgttaccggc tttacgtgct tcccggctga atatttctga
    10801 cgcatttaaa ttctcatagg gctaattatg aatatggctt tttacggcaa gtggtttgct
    10861 tgcctgtggc tcgctacgtc gtgcgttcag gcggcgagca cagataataa ggcgctggag
    10921 ataattcgtc gcgctgacga aattcgttcg ccgaataaac cgtttcgtta cacgctgacg
    10981 gtaacggaat ataaagcggg cgcaacccag ccagagaata aacaggtcct cgatatttcc
    11041 atgcgcttta tgaaaccgca gggtaatgaa aaagcggatg cacgctcgct ggtgcgtttt
    11101 atttatccgc cgcgagataa aggaaaaatc atgctttccg actggtacga tttatggttt
    11161 tacacgccgg aactgcgacg tcctatgcct atttcacggc aacaaagatt aattgggcaa
    11221 atctctaatg gcgatgtcat tgtcactaac tttgaatatg cctatgactc gaccctgatg
    11281 ggggaagtca cgtgtgctga aaagcagtgt tacaagctgg cgctggtgcg taaatcggcg
    11341 gatatcacct ggccgaaggt tatctattac gttgaaaaag acggtgataa tcgcccgtgg
    11401 aaggcggcct attattcgca ggatgaccag ttaataaaag aagtgttgta tcaggatttc
    11461 cagccggtgt tggggaaaac gcgacccatg aaaataaccg ttaccgatgt tcgtcacggc
    11521 aataactatt cagtgatgga gtacagcgat gttcgtctgg aatcgctgcc ggagtttcat
    11581 ttcaccaaag agtatattca gcgaggtgcg aaatgaaacg tacgctgaag atctcttcgt
    11641 tgctgtgtgt tgcgctgccg ttgacggtac aggcggattg cctgtcaggt gacgaggtgg
    11701 cacaaaatag cgatgtgacg ctttacgagc aggtgtctta tgtcaataaa caggcttcgg
    11761 cctggcagat tgcaggcaaa aatccctaca cccgtcacaa cggttatcag gaggcgggga
    11821 ttggcatcaa tagcggttgt tcgataatcg acaatacgct ggaccttaag ctcaatctgt
    11881 atggaataaa cgaatatgcg cttaaacccg cagggaaatt cgaaacggat gatagccgca
    11941 cgcgtgcgtt aattaaccgc ctcagtctgg tctatagcgc ctcggacagc gttcaatttg
    12001 aagcaggcaa atttgccgcg ccgtcaggaa tgttcttttt gcgttcgcct tctgatttgc
    12061 aaacgcatta ttacaccgga ttccagtcga cgcgtttgca cgatcccaaa atgacatcgg
    12121 cgtatcaggc gtcatcgtgg ggcgcaaaaa tgagcgtgga tacacgcgat tatgcgtttt
    12181 cggcgagtgt cataccgaag ttagccacca tcgacaaacg ctatgtgacg tctggcaact
    12241 ggtcggctaa tcagcaggga aacagcgacg aagcctatct gctgagcttc agcgatcatc
    12301 gctttggtga gcacacgccg acagtcaatg tgcggctcgg accatcgccg tcactcgcgc
    12361 tgtctgacag ttatcactac accccgcagc ttacgctgaa tgtcgacgca gcctaccacc
    12421 gctcccagca atggcggcat ctttcccacc gtgagacggc gcaggtcgag gaatatcagt
    12481 ttccggattc gctctatgaa acgaaagatg aaagcggcgt agagctggcg ctgggtgggc
    12541 agtacaccag tgataatttc agcgtgtttg gcgttgagta ttactttcag agcgagggtt
    12601 attcgcgcgc tgaacagcgt cagcagcgcg agttaattga cttcctcaat accacaaccg
    12661 gctatgcgcc actggatcag gcatttgatt cttacaaata cctgatggct tcagaaatca
    12721 gcaataccgc caatcagggg atgctgcagg gtaagcacta tctgaacgcc tgggccagtc
    12781 tgccgctggc gggagagtcg acgctgcagc cttcgctggt ggtgaatctc gttgatggca
    12841 gcacgttgct gggcctgcat tattccacgc cgctcagcgc tataagtaac cagcttgaag
    12901 cctatgccgg tggctatagc gcgctgggaa gtcggtactc cgaatttgcg ctttttggcg
    12961 acacgttagg cctttatttg ggctttaaat actatctata aggataaaga gatgatgaat
    13021 aatgcttcac aaccgacagt actgcttttt gccggtcagg gtaatccggt tattggtatg
    13081 ggcagcgatc tttgggatct caacgccagt acaaaacata tctgggattg tgccagcgat
    13141 atttccggac tgaacctgcg tcgcctgtgt cagaaagggc cgatgaatcg ccttattcaa
    13201 acgacggtac aacaactggc ggtgaccgca attaatgtaa cgctctatac cctgtgccgc
    13261 gagcgtcttg agaatttaac ggttgtgggc tcctgcggac acagcgttgg tgaatacagt
    13321 gcgctctacg ccgctggagc tatttctctt gagacgctat ttggcatgat tcacttcaga
    13381 gccagcctga tgcatgacga aagccagaga aataaaggcg caatgctggc ggttaaaggc
    13441 atcaaccgcg aacaactgca gacgatgatt acggaatcag gcattgcgct ggatattagc
    13501 tgtgataaca cgccgcgcca gcaggttatt gggggaacgc aggctgcgtt gaatgaattt
    13561 gccacgctgc tgatggccgc tggatacgaa ccggttaaac tcggtgtgag cggtgcctgg
    13621 cacacgcgtt tgatggaaga cggcgttcag gcaatgcgcg attatcttgc cggactggat
    13681 atcgcttcgc cagagcatca ggttctgatg aacgtaacag caaaaagcga ggtcgctccg
    13741 tcaattatca aagaaaatct ctcattacac ttaactcata cggtgaaatg gactgaatct
    13801 ctcgatacat ttctgaatat gccaacctcg gtagctttcc ttgaaataag taataagccc
    13861 tatttgggga atatgttcaa cgatttcgct ggcgtggatc agcaacgagt tatgcattgt
    13921 cgtaaagcat ttagtgatgc gaaggtattc aaatgattcc tgattattta acttttattc
    13981 gctttcagga taaacgaagt ctgatataca tttatgctat tggacttatt ctgataggct
    14041 tttattggaa aaatgcagga tttacttttc catcagagga tattggtgtc gttagtggga
    14101 ttctggccct ggtgctgtat aattttattt ttgatctcaa ggcgtactgg gcttataaat
    14161 gcgtcacgaa gaatatcgat ttttcgtggt ttaagaaaaa gcagaaccac aaaatagaat
    14221 tatttcttac gcaacctctg gtggcaggat ttctgtcgtt aatcatgttg agtgcaatga
    14281 gttgggggct ataccagcgt ctaccctcgt tatatgcgct gttcctgatt tcgttacttg
    14341 ggccgttggt tatctttctg ctgtttcgga tgatccgcac cagttatgtc aagcaggtcg
    14401 ctatttcagt agcgaaaaaa gtaaaatata aaagtctgac tcgctatgtg ctgctttcgg
    14461 tgtgcatctc aacggttgtt aacctgctta ctatcagccc gttgcgtaac agtgattctt
    14521 ttgtgataga ggggcagtgg ttaacgttta aatcgataat tgcattgctc attctttgtg
    14581 gcgtagtgtt ggcgattaat ctgttttttc tgcgcttctc caggcggtac gcttttttgg
    14641 gcaggctttt tttgcaggaa atcgatctct ttttctccag tgaaaatgca ttgtcgacct
    14701 tttttgccaa gccgctttgg cttcggttat tcatattgct ggttattgaa gtgatgtgga
    14761 ttacgctggt gtcggtattg gcaacgcttg tggaatggcg gatttggttt gaagcctatt
    14821 ttttactctg ctatgtaccg tgcttaattt actatttttt ctattgtcga ttcctctggc
    14881 ataacgattt tatgatggca tgtgacatgt atttccgttg ggggcatttt aataagtgag
    14941 gaagtgatag gaagtgacca gataatacat atatgttctg tactctcttg cgcattttga
    15001 ttgttgactg agcaaccaga cagttgatgt gcacgatttc ccctcgccca aacagacgtg
    15061 ggcgggggca cccggtgtgt gcaatatacg aaaaaaaagc ccgtactttc gtacgagctc
    15121 ttctttaaat atggcggtga gggggaccgc ctgaaataaa tctgacttaa tccaggggtg
    15181 ttatataatt tcattataaa caatgtatta cactttttgt tgtccgatat tatctaatgt
    15241 attcttagtg aagcgagaga ctaatggcat actggatggc ataccagagt gacgggaatt
    15301 agcgtatggc agtattgacg gatacgaaag caagacatat caaacctgat gacaaaccat
    15361 tgccccatgg gggaattacc ggactgaccc ttcatccttc ttcagtaaag gggcggggga
    15421 aatgggtttt tcgttatgta agtccggtga cacaaaaaag acgtaatgct ggattgggaa
    15481 cttacccaga ggtcagtatt gctgaagctg cacgtactgc ccggataatg cgagagcaac
    15541 ttgctgcagg tgatgatcct ctggagatta aaaaggctga atctgagaaa gtcgctatcc
    15601 caacatttgc cgatgcagcc aggcgtgtac atgcagaact gtctcctgga tgggaaaatc
    15661 caaagcatgt aaggcagtgg ttatcgacgc ttgagaatta cgcgtttcct caactgggag
    15721 caaaaacgct ggattcgatt acggctgcgg acgtggcaga aacactgcgt ccagtctggt
    15781 taaccttgtc agaaacggca agccgggtta aacagcgcat tcatgttgtt atgcagtggg
    15841 gatgggcgca cggtttttgt gtagcaaatc ctgttgatgt ggttgaccat ttgcttcctc
    15901 agcagacaag aggacgtgat gaacaccaac ccgcaatgcc ctggaggcag ttaccgcttt
    15961 ttgtggcgac cagtgtgtat accgatgaac cttataatgt tacccgcgca ctgttattaa
    16021 tggtgatact tacagcaact cgctcgggcg aagccagggg aatgcgctgg gctgaaattg
    16081 attttcataa gcgggtatgg actatacctg cagaaagaat gaaagccagg atacagcatc
    16141 gtgttccttt atcccggcag gctatttaca ttctggaaaa tatacgtggc ctgcatgatg
    16201 aactggtgtt cccttcaccc agaaagcagc agatcctttc cgatatggtg ttgacaagtt
    16261 ttctgcgtaa aaagaaagcc gtcagtgaca ttccggggcg agttgccacg gcacatggtt
    16321 ttcgctcaac attcagggac tggtgtagcg aacaggggta ttcgcgggat ctggcggaaa
    16381 gggcgctcgc tcatacgctg aaaaataagg ttgaggcggc atatcatcgt actgatctac
    16441 tggagcagcg tgtaccgatg atgcaggcat gggcggatta tgtgatgtct caaattgtga
    16501 ataaataata gtgaaaatgt atattgtacg atcat
//
LOCUS       DBFIFI010000090        16460 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.090, whole genome shotgun sequence.
ACCESSION   DBFIFI010000090 DBFIFI010000000
VERSION     DBFIFI010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.090"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..93)
                     /locus_tag="N7B39_002776"
     CDS             complement(<1..93)
                     /locus_tag="N7B39_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2422730.1"
                     /translation="MILSTRISLGLFFKYFLSLTKIDPGQNYISL"
     gene            complement(240..1523)
                     /gene="yeaH"
                     /locus_tag="N7B39_002777"
     CDS             complement(240..1523)
                     /gene="yeaH"
                     /locus_tag="N7B39_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HGU2422731.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            complement(1636..3570)
                     /gene="yeaG"
                     /locus_tag="N7B39_002778"
     CDS             complement(1636..3570)
                     /gene="yeaG"
                     /locus_tag="N7B39_002778"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HGU2422732.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            4006..4752
                     /gene="mipA"
                     /locus_tag="N7B39_002779"
     CDS             4006..4752
                     /gene="mipA"
                     /locus_tag="N7B39_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HGU2422733.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNSNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPIVDKSWTGLISTGITYKF"
     gene            4842..5696
                     /gene="yeaE"
                     /locus_tag="N7B39_002780"
     CDS             4842..5696
                     /gene="yeaE"
                     /locus_tag="N7B39_002780"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HGU2422734.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAVMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELAMLDKAYPAPK
                     GKTALDMV"
     gene            complement(5747..6631)
                     /gene="yeaD"
                     /locus_tag="N7B39_002781"
     CDS             complement(5747..6631)
                     /gene="yeaD"
                     /locus_tag="N7B39_002781"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HGU2422735.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDANGVALTFELTQSEETKKFWLHDFTLLAHFRVDKNCEIDLEAHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASEMQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(6715..7710)
                     /gene="gapA"
                     /locus_tag="N7B39_002782"
     CDS             complement(6715..7710)
                     /gene="gapA"
                     /locus_tag="N7B39_002782"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HGU2422736.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            8052..8465
                     /gene="msrB"
                     /locus_tag="N7B39_002783"
     CDS             8052..8465
                     /gene="msrB"
                     /locus_tag="N7B39_002783"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HGU2422737.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            8507..8779
                     /gene="yeaC"
                     /locus_tag="N7B39_002784"
     CDS             8507..8779
                     /gene="yeaC"
                     /locus_tag="N7B39_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HGU2422738.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARNNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            9149..10225
                     /gene="ydjL"
                     /locus_tag="N7B39_002785"
     CDS             9149..10225
                     /gene="ydjL"
                     /locus_tag="N7B39_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HGU2422739.1"
                     /translation="MKALARFGKAFGGYKMIDVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            10252..11631
                     /gene="ydjK"
                     /locus_tag="N7B39_002786"
     CDS             10252..11631
                     /gene="ydjK"
                     /locus_tag="N7B39_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2422740.1"
                     /translation="MDQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMGVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRHTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLIAM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGTPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            11648..12691
                     /gene="ydjJ"
                     /locus_tag="N7B39_002787"
     CDS             11648..12691
                     /gene="ydjJ"
                     /locus_tag="N7B39_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HGU2422741.1"
                     /translation="MKNSKAILQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVH
                     GFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRKFKPGDRVNIEPGVPCGHCRYCL
                     EGKYNICPDVDFMATQPNYRGALTHYLCHPESFTYKLPDNMDTMEGTLVEPAAVGMHA
                     AMLADVKPGKKIIILGAGCIGLMTLQACKCLGATEIAVVDVLEKRLAMAEQLGATVVI
                     NGAKEDTIARCQQFTEDMGADIVFETAGSAVTVKQAPYLVMRGGKIMIVGTVPGASAI
                     NFLKINREVTIQTVFRYANRYPVTIEAISSGRFDVKSMVTHIYDYRDVQQAFEESVNN
                     KRDIIKGVIKISD"
     gene            12712..13548
                     /gene="ydjI"
                     /locus_tag="N7B39_002788"
     CDS             12712..13548
                     /gene="ydjI"
                     /locus_tag="N7B39_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HGU2422742.1"
                     /translation="MLADIRYWENDATNKRYAIAHFNVWNAEMLMGVIDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIKLFGSDGK
                     AE"
     gene            13553..14485
                     /gene="ydjH"
                     /locus_tag="N7B39_002789"
     CDS             13553..14485
                     /gene="ydjH"
                     /locus_tag="N7B39_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HGU2422743.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMTTGGDA
                     INEATIISRLGHRTALMSRIGKDAAGQFILDHCRKENIDIQSLKQDVNIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFSQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMNICADMIKPRLNETLDDICEALSYVNYAEAKLLTGKETLDEIADSFLACGVKT
                     VVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNLRECAR
                     FANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            14495..15475
                     /gene="ydjG"
                     /locus_tag="N7B39_002790"
     CDS             14495..15475
                     /gene="ydjG"
                     /locus_tag="N7B39_002790"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HGU2422744.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTAPGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGFDYIDIYMTHWQSVPPFYTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEVLGR"
     gene            15612..16370
                     /gene="ydjF"
                     /locus_tag="N7B39_002791"
     CDS             15612..16370
                     /gene="ydjF"
                     /locus_tag="N7B39_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGU2422745.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAMLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALESNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
BASE COUNT         4287 a         3696 c         4154 g         4323 t
ORIGIN      
        1 cagagatata tagttttgac caggatcaat tttcgttaac gataaaaaat atttaaagaa
       61 taaccccagg gaaatacgtg ttgacagaat aatttttccg aatgattgtt ttctggaact
      121 aaattgtgta aaggttatgc tgtcatattt aaagcgattg taagctaatg tatgtaataa
      181 atgagataac ttacagtgag ctaaaaatca gccaggcgat aatgacccgg ctgatagttt
      241 tagtctttag ctgttgcatt ttgcttatga aacagttcac ggaacaccgg ataaatatca
      301 tcctggtcgc ggatatgctg catcgcaaag ttgtcgaaag tagattgcag atgctcatat
      361 tctcgccaca gtgtctgatg cgcgcgacgg gtaatttcga tatagctgta ataacgaaca
      421 acgggtaata attttttcgc cagaatttca tggcaaagcg gagagtcatc ggcccagtta
      481 tcgccgtccg atgcttgtgc agcgtaaata ttccactgtg ccgggttata acgctctttc
      541 actacctcat ccatcagttt cagggcgctg gaaacaatgg tgccgccagt ttcctgcgag
      601 tagaaaaact catgttcatc gacttctttc gcctgggtat gatggcggat gtatacgact
      661 tccacgttct tatacgttct gctgaggaac agatacagca gaatataaaa acgcttagcc
      721 atatctttag tggattgatc cattgaaccg gaaacgtcca tcaggcaaaa catcactgcc
      781 tggctggagg gatcgggccg cttctcgtag ttcttgtaac gtaaatcgaa ggtgtcaata
      841 aaagggacgc gttcaatttt ggcacgtaat tctgcaattt ctttgcgcag acgttcctct
      901 tccagcagct gcgcaggttc actgttgctg atgatggcca aattctcttc cagtgcatga
      961 agttcccgcc gcttgcctgc cgtcatggct gtgcgtcgcg ccagtgagtt ctgcaaggaa
     1021 cgcacaacgc tgatattggc cggaacgccg ttagcggtat aacccgcccg atgcgtttta
     1081 tattcggtca gctggcgttg ttggttttgt ttcagattcg gtaaggccaa atcttcaaag
     1141 agcagatcaa gatactcatc tttcgaaatc tgaaagacaa attcatcctg accttcacca
     1201 tcctggctgg cctggccctg accactgccg gaacctccgc cgccaccctg gggacgttca
     1261 attcggtcgt tctgaacgaa atggtcattg cccggatgca cgcggtggcg cagaccgcca
     1321 cgcccctgat gaaacattgg ttcgctaata tcttccgtgg gaatggatac ggactcgccg
     1381 ctgtcgacgt cagtcaccga acgcttatta atggcctcgg agatcgactg tttaatttgc
     1441 gctttataac ggcgtaaaaa acgctggcga ttcaccatgc ttttgttttt gccgttcaga
     1501 cgccggtcaa taaaccaggt catatgcccc ccgtactaca tttgccaact gtatgctacg
     1561 cctgtgttgc catttctctg gcccacggtt cgctgtcgtt gtaggcccgg tagcatgtgt
     1621 accgggccag ggttgttaag acgatttacg tacgcgcaaa taccattcgc acagtaaacg
     1681 cacctgttta cgggtgtagc ctttttccat catacggtcg acaaagtcgt cgtgtttctt
     1741 ctgctcgtcg gttgacgttt tggcgttaaa cgagataacc ggcaacagct cctcggtatt
     1801 ggagaacatt ttcttctcga taaccgtgcg cagtttttca tagctggtcc agttcggatt
     1861 gcgtccgctg ttattggctc tggcgcgcag tacgaagtta acaatctcgt tgcggaaatc
     1921 ttttggatta ctgatccccg ccggtttctc gattttctcc agctcggcgt taagagactc
     1981 gcggtcaaac agctgcccgg tatccggatc gcgatactcc tgatcctgaa tccagaaatc
     2041 cgcgtaggta acataacggt cgaaaatgtt ttgcccatat tcggaatagg attcaaggta
     2101 ggccgtctgg atctctttgc caataaactc ggcatatttc gggatcagat aacctttcag
     2161 gaactccaga tagcgttctg cctgctcttg tgggaactgc tcgcgctcga tctgctgttc
     2221 caggacgtag aacagatgga ccgggtttgc cgccacttct acatgatcga agttgaacac
     2281 gcgggagagg atcttaaacg caaaacgcgt cgacagaccg ttcatacctt catcgacacc
     2341 ggcgtagtca cgatattcct gatacgactt ggctttggga tcggtgtctt tcagactttc
     2401 gccatcataa acccgcatct ttgaataaat gctggagttt tctggctctt tcaggcgcga
     2461 aagaatggaa aaacgtgaca gtgtttcgag agtgccaggg gcgcatgggg cgtgagtcaa
     2521 ttcactgtga ttaagcaatt tctcgtagat tttgatctct tcggaaatgc gcaagcaata
     2581 cggcaccttc acgatgtaaa cacgatcgag gaaggcttcg ttgtttttgt tattacggaa
     2641 agtgacccat tcggactcgt tcgagtgggc aagaataatc ccgttgaacg gcagggcgga
     2701 gataccttcc gtcccgttgt agttaccttc ctgagtggcg gttaacaagg gatgcagcac
     2761 ttttatcggt gctttaaaca tctcgacaaa ttccatgatc ccttggttgg cgcggcacag
     2821 cgcaccggaa tagccgtagg cgtccgggtc attctgcgcg taatgttcga gtttacgaat
     2881 atcgactttc ccaaccagcg cggagatgtc ctggttgttc tcatcaccgg gttccgtttt
     2941 ggcgatagca atttgttgca gaattgacgg ccagaccttc actacccgga acttagtgat
     3001 atcgccacca aattcatgca ggcgttttgc cgcccacggc gacatgatgg tgccgagata
     3061 acggcgagga atgccatact ctttttccag aatctgcgca tcttcctgcg gattgaaaag
     3121 acagaacgga tgatcgttga ccgggctacg ctcaccgttg gcgctcaata cataaatcgg
     3181 tacgagctgc attaatgatt tcagtcgctc agcaagcgat gatttacccc cacccacagg
     3241 ccccagcaga tacaggattt gtttcttctc ttccagcccc tgagccgcgt gtttcagata
     3301 agagacaatc tgttcaatcg cgtcttccat gccgtaaaac tcttcaaacg ccggataacg
     3361 tgcaatgacc cggttagaaa agagtcgaga aagtctgggt tcctgggctg tatcgaccat
     3421 gacaggctca ccgatagcca tcaatagccg ctcagccgcg ttggcataag cactgcgatc
     3481 ttgccgacaa gtggtaagaa actcctgcag tgtgaactct tcgtccttgg cagcttcata
     3541 tcgctggcga tagtgatcga atatattcat ggtatgccgt cctttcgttt tttagcacag
     3601 gttaagagcc gttcatatga atagtagagg ctcccggaag aggtaaactg aacgacgtct
     3661 cttgctaaaa cacagcaact ctcatgccat tttatcgacg atgagaacaa cgtgcccggt
     3721 gatacacctt caaaattaag cgtagatggc aaatggaaaa cttgcctaca cggctttctt
     3781 aatttcaatg gcatatcaac aactcatcca taaattttgc ataattaatg taaagaccag
     3841 gctcgccagt aacgctaaat tcatttggct gtaagcgcgg tgtcatccgc gtcaggaaaa
     3901 ttaaacagtt actttaaaaa atgaaaacgt aaaaaggttg ggtttcgatg tattgacggg
     3961 taaactttgt cgcccgctaa acatttgttt aaggaatgat taattgtgac caaactcaaa
     4021 cttctggcac ttggagtgct tatcgcaacg tctgcaggcg tagcgcacgc tgaaggtaaa
     4081 ttttccctgg gcgcaggcgt aggtgtcgtt gaacacccat ataaagatta cgataccgat
     4141 gtttacccag taccggtaat caactatgaa ggcgataact tctggttccg tggcttaggt
     4201 ggtggttact acctgtggaa tgacgcaacg gataaacttt caattaccgc ttactggtcg
     4261 ccgctttact tcaaagctaa agacagtggc gatcaccaaa tgcgtcacct ggatgaccgt
     4321 aagagcacca tgatggctgg tctgtcttat gctcacttta cccagtacgg ttacctgcgt
     4381 accaccctgg ctggcgatac cctggataac agcaacggca tcgtctggga tatggcctgg
     4441 ttgtatcgtt acaccaacgg tggcctgacc gtgactccgg gtattggtgt gcagtggaac
     4501 agcgaaaacc agaacgaata ctattatggc gtatcgcgca aagagtccgc tcgcagcggt
     4561 ctgcgtggct ataactcgaa cgacagctgg agcccttacc tggagctgag cgccagctac
     4621 aacttcctcg gcgactggag tgtttacggt accgcgcgct acacccgtct gtctgatgaa
     4681 gttactgaca gcccgattgt ggataaatcc tggactggcc tgatttctac cgggatcacc
     4741 tacaaattct gataatgcat ctttccaggg cattttgtgc ataaaaacag ggcgtcacgc
     4801 cctgttttgc attacggtgg tgcaatacct gggaggaaac aatgcaacaa aaaatgattc
     4861 aatttagtgg cgatgtctca ctgccagcca tagggcaggg aacatggtat atgggcgaag
     4921 atgccagtca gcgcaaaaca gaagttgctg cactacgcgc gggcattgaa ctcggtttaa
     4981 ccctcattga taccgccgaa atgtatgccg atggcggtgc cgaaaaggtg gttggggaag
     5041 cattaaccgg tctgcgagag aaggtctttc tcgtctctaa agtctatccg tggaatgctg
     5101 gcgggcaaaa agcgataaat gcatgcgaag ccagtttacg ccgtctcaat actgattatc
     5161 tcgatctcta cttattacac tggtctggca gtttcgcttt tgaagagact gttgcagtga
     5221 tggaaaaatt gatcgcccag ggaaaaatcc gccgctgggg cgtttcgaac cttgattatg
     5281 ctgatatgca ggaactctgg cagctgccgg ggggaaatca gtgtgccact aatcaggtgc
     5341 tttaccatct cggttcacga gggattgagt acgatctact cccctggtgc cagcaacagc
     5401 agatgccggt gatggcttac agtccgttag cccaggccgg gcggttgcgc aatggactgt
     5461 taaaaaacgc ggtagtcaac gaaattgcac atgctcacaa tatcagcgcg gcgcaagtat
     5521 tgttggcgtg ggtaatcagc catcagggtg tgatggcgat tccaaaagcg gccacgattg
     5581 cccatgtcca acagaatgcg gctgtgcttg aggtcgaact ttcgtcagcg gaattagcca
     5641 tgctggacaa agcgtatccg gcaccaaaag gaaaaactgc gctggatatg gtgtgatgtt
     5701 gatacgtgcc ggatgcatcg cacccggcac acaacattga cgcaaattaa cgtttggcaa
     5761 cgcgaatgga ttgcgccaga tgtgcaggtt tttctttggt cactttttgc atttctgagg
     5821 cgtaagctgt ttctacacaa acaaaggtct tgtagccatc gtccggcatg tcgcccatgc
     5881 taactgaaag cgccggtccc gggttccagc ctacaacgtt cagatgatgc tggtggccta
     5941 cggcgataat acgattcagc gcttcatcat taatcacgct gcaatcttgc ggattcagat
     6001 acacgcgatc ggtacggtca gggaaggtct gaataccgtc ggtcagtaca tcttctttcg
     6061 cgtcatttac tttatcaatg aagcgatcgc ccagcccact gacgcttacc ttagcgatat
     6121 cacccacgtt aaagtaggta tgcagggcag aggtggtttc aaattcgccg tgtgcttcaa
     6181 gatcgatttc acagttttta tccacgcgga aatgcgctaa cagagtaaag tcgtgcagcc
     6241 agaatttttt cgtctcttcg ctttgtgtca attcaaaagt cagcgctacg ccattagcat
     6301 cttcacgatg tgatttcagc gtccacggca ggttgcgggc aaaaccgtgc gcaggcaggc
     6361 cttgttgtgc cgccggacca aaccacggcc agcaaaccgg tacgccaccg cgaatagcaa
     6421 cgccattttt gaacggtgtg ttgttgctca accacagaac ttcttcttca cctgcaggtt
     6481 tccacgagag aaggtgtgcg ccctgtaatg caaaagaggc ttttacctgg ggatgatcga
     6541 ctacaatgag gtccagttca tccagtttac gacgggagag gacaggggag atttgttcga
     6601 tgaccggaag ggcaaaaatt ttcttaatca tgacgcagtc ctttaacttc attttatcag
     6661 gtaaaaaaaa gagcgaccga agtcgctctt tttagatcac agtgtcatct caacttattt
     6721 agagatgtga gcgatcaggt ccagaacttt gttggagtaa ccggtttcgt tgtcgtacca
     6781 ggataccagt ttcacgaagt tgtcgttcag agcgatacca gctttagcat cgaacacgga
     6841 agtgcaaact tcgccgttga aatcggtaga tactacgtca tcttcggtgt agcccagaac
     6901 gcctttcatt tcgccttcag cagcagcttt aacggcagct ttgatctgct cgtaagttgc
     6961 agctttttcc agacgaacgg tcaggtcaac tacggatacg ttcggggtcg gaacgcggaa
     7021 cgccatacca gtcagtttgc cattcagttc tggcagtact ttacctacag ctttagcagc
     7081 accggtagag gacgggatga tgttctggga agcgccgcgg ccgccgcgcc agtctttgtg
     7141 agacgggcca tcaacggttt tctgagtagc ggtagtagcg tgaacggtgg tcatcagacc
     7201 ttcgatgatg ccgaagttat cgttgataac tttagccagc ggagccaggc agttggtggt
     7261 gcaggaagcg ttggaaacga tgtcctggcc agcatatttg tcgaagttag cgcctttaac
     7321 gaacatcgga gtgttgtctt tagacggacc agtcataacc actttcttcg caccagcggt
     7381 gatgtgttta cgagcagttt cgtcagtcag gaacagacca gttgcttcag cgacaacgtc
     7441 aacaccaact tcgtcccatt tcaggttagc cggatcacgt tcagcggtaa cacggatttt
     7501 tttaccgtta acgatcagat gaccgtcttt cacttcaacg gtaccgtcga aacggccgtg
     7561 agtggagtca tatttcagca tgtatgccat gtaatcagcg tctaacaggt cgttgattgc
     7621 aacgatctcg atgtcagaac gtttctgagc agcacggaaa acaatgcgac cgatacggcc
     7681 aaaaccgttg atacctactt tgatagtcat atattccacc agctatttgt tagtgaataa
     7741 aaggttgcct gtaaaattac aaaaacctta cgcagcgtca agcggaatcg tgtcaatcat
     7801 tgcgacaaat caatcctgtg cctaagcatt acgcgactga ctcgcctcac tcttcctttg
     7861 ggcttgagac cacatggggt cggcgccccg aattttaaag ggcaattacg ataaaaatgt
     7921 gattttcatc acgatttagg tgcagccatt tcgtcgagat caagtttagg acaaaatttt
     7981 gccagttggt gttaatgttt tgttagaatc agtcacgtaa tgtgagcacg attaaagtga
     8041 gatgtgagca aatggctaat aaaccttcgg cagaagaact gaaaaaaaat ttgtccgaga
     8101 tgcagtttta cgtgacgcag aatcatggga cagagccgcc atttacgggt cgtttactgc
     8161 ataacaagcg tgacggcgta tatcactgtt tgatctgcga tgccccgctg tttcattccc
     8221 aaaccaagta tgattccggc tgtggctggc ccagtttcta cgaaccggtg agtgaagaat
     8281 ccattcgtta tatcaaagac ttgtcacatg gaatgcagcg catagaaatt cgttgcggta
     8341 actgtgatgc ccatctgggg catgtcttcc ctgacgggcc gcagccaacg ggcgaacgtt
     8401 attgtgttaa ctctgcttct ttacgcttca ctgatggtga aaacggcgaa gaaatcaacg
     8461 gttgaaaaaa cgattcagca acttattcca caggagcgga aaaattatga atcttgatga
     8521 cattatcaac agcatgacgc ctgaggtata ccagcgtttg tcgaccgccg ttgaactggg
     8581 gaaatggcct gatggcgttg cgttgaccga ggaacaaaaa gagaactgcc tgcaactggt
     8641 gatgctgtgg caagcccgca ataataccga agcacagcat atgacaattg acacaaatgg
     8701 tcagatggtc atgaagagca aacagcagtt aaaagaagat tttggtatct cagcaaagcc
     8761 tattgccatg tttaagtaag tggttcatcc cctttttgcc gcggctatga aggggatagc
     8821 caacaatgac cccacgactc atctcaatta tctcgccatg acctggctaa gtcccagtcc
     8881 gatctcttgc cgtcgcgttc acgcctgaat gacttctcct gaacttaagt ctcagaccta
     8941 tttggccggt aatccctctc aaatttgctc cttttgcgtt ccgccttaca tcaacctgta
     9001 acagaaccag ccattttctg cgtaattgtg atctgagttg taaaacgcag attattatct
     9061 gtgttttaca tgtttaatgt tgacctcaaa aatcatgtgg gtaagtatga tgaccatcta
     9121 agcaaaacaa aacacagagg atgacgcaat gaaagcactg gctcggtttg gcaaagcctt
     9181 tggcggctac aagatgatcg atgtgccaca acccatttgt ggcccggaag atgtagtgat
     9241 tgaaattaaa gccgcggcaa tctgcggcgc agacatgaag cactacaatg tcgatagcgg
     9301 ttctgatgag tttaactcta tccgcggcca tgagttcgca ggttgtattg cgcaggttgg
     9361 tgaaaaagtc aaagactgga aagtggggca acgcgtcgtg tcggataaca gcggtcacgt
     9421 ctgcggcgtt tgtccggcct gtgaacaagg tgattttctg tgttgtacag aaaaagtaaa
     9481 ccttggtctg gataacaata cctggggcgg tggtttttcc aaatattgtc tggttcctgg
     9541 tgaaattctc aaaattcatc gtcatgcgtt gtgggaaatc cctgatggtg ttgattatga
     9601 ggacgcagcc gtacttgacc ctatctgtaa tgcctacaaa tccatcgcgc agcaatcgaa
     9661 attcctccct ggtcaggatg tggtcgtcat cggcactggc ccactcgggc tgttctccgt
     9721 acaaatggcg cgaattatgg gggcggtaaa tatcgtcgtc gttggtctgc aagaagatgt
     9781 ggcggtccgc ttcccggttg caaaagaact gggtgcgacg gcagtagtaa atggttctac
     9841 cgaagatgtg gtggcgcgct gccagcaaat ttgtggcaaa gacaatctgg gactggtgat
     9901 tgaatgctcc ggtgccaata tcgcactgaa acaagccatc gaaatgctcc gtccgaacgg
     9961 ggaagtggta cgcgttggaa tgggcttcaa acctcttgat ttctcgatta atgacattac
    10021 cgcctggaac aaaagcatca ttgggcatat ggcctatgac tccacctcat ggcgtaacgc
    10081 tatcaggcta ttagccagcg gcgctatcaa agtcaaaccg atgatcacgc atcgtatcgg
    10141 cctgtcgcaa tggcgcgaag ggtttgatgc gatggtcgat aaaaccgcaa tcaaagtgat
    10201 catgacttac gactttgatg aataaccact ctcctgaagg aacgttatcg catggatcag
    10261 ataacaaaac cgcattgtgg tgcacggctg gatcgcttac cggattgccg ctggcattca
    10321 tcaatgtttg ccatcgtcgc gtttggcttg cttgtctgct ggagtaatgc cgttggtggc
    10381 ttgatcctcg cgcagctgaa agcgttgggc tggacagata attccaccac agccacattc
    10441 tcagcaatca cgaccgccgg aatgtttctc ggtgcgctgg tgggcggcat cattggcgac
    10501 aaaaccggtc gcagaaatgc gttcatcctc tatgaggcca ttcatattgc ctcgatggtt
    10561 gtcggtgctt tctcaccgaa tatggatttc cttattgcct gccgttttgt gatgggagtt
    10621 gggctgggag ctttactggt tacgctgttt gctggtttca ccgaatatat gcccggtaga
    10681 aatcgtggaa cgtggtcaag tcgggtttcc tttattggca actggtcata tccgctctgt
    10741 tcattgatag cgatgggact cacgccgctg attagtgcag agtggaactg gcgggtacaa
    10801 ctgcttatcc ctgcaatatt gtcgcttatc gctacggcgc tggcctggcg ctactttcct
    10861 gaatccccgc gctggctgga atcgcgcgga cggtatcagg aagccgagaa agtcatgcgg
    10921 agtatagaag aaggcgtcat acgccataca ggtaaacctt tgccgcccgt ggttattgct
    10981 gatgacggta aagcaccaca agcggtgcca tattcagcct tactgacagg agtattactg
    11041 aaacgcgtga tattaggctc ttgtgtgctg attgccatga acgttgtgca gtacacacta
    11101 attaactggt tgccaacgat attcatgacc caggggatta atttaaaaga ctcgattgtt
    11161 ttaaatacca tgagtatgtt tggcacgcca tttggcattt ttattgccat gctggtgatg
    11221 gataaaattc cgcgtaaaac aatgggtgtg gggctattaa tcctgattgc ggtgctcgga
    11281 tatatctatt cactgcaaac cagcatgttg ctcataacgt tgattggttt cttcctgatt
    11341 actttcgtct atatgtacgt ttgctatgcc tcggcagtgt atgtccctga gatctggccg
    11401 acagaggcca aactccgtgg ctccggtctg gcgaatgcgg tagggcgaat cagtggtatt
    11461 gccgcacctt atgccgttgc agtgctgctc agtagttatg gcgttacggg agtctttatt
    11521 cttctggggg cggtttcaat tattgtcgca attgctatcg ccaccattgg aatcgaaacc
    11581 aaaggtgtct ccgttgaaag tttaagtatt gatgcagtag ccaataaata atacgggtgg
    11641 attcaaaatg aaaaattcaa aagcaatatt gcaggtgccg ggcacaatga aaattatttc
    11701 agcagaaata ccagtgccta aagaagatga agttttgatt aaagtagaat atgtcggtat
    11761 ttgtggttca gatgtacatg gttttgaatc aggtccgttt attccgccta aagacccaaa
    11821 tcaagaaatt ggcctgggtc atgaatgcgc cgggacggtt gtggctgtgg gaagccgtgt
    11881 gcgcaaattt aaaccggggg atcgggtaaa tatcgaacct ggcgttcctt gcggtcactg
    11941 tcgttactgt ctggaaggca aatataacat ctgcccggac gttgatttta tggcgacaca
    12001 acccaactac cgcggcgcat taacgcacta tctgtgtcat ccggagagct ttacttacaa
    12061 actgcccgac aatatggaca cgatggaagg gacgctggtg gagcctgccg cagtcgggat
    12121 gcatgccgcg atgctggcag atgttaaacc gggtaagaag ataattattc tgggagcagg
    12181 ttgtattggt ttgatgacgt tgcaagcgtg caaatgcctg ggagcaacgg aaattgccgt
    12241 cgttgatgtg ctggaaaaac gtctggcaat ggcggaacag cttggtgcga cagtggttat
    12301 taacggcgca aaagaagaca ctattgcacg ctgtcagcaa tttaccgaag acatgggcgc
    12361 agatattgtt ttcgaaacag cgggttctgc ggtcaccgtt aaacaggcac cttatctggt
    12421 aatgcgtggc ggtaaaatta tgattgttgg tactgtaccc ggcgcttcgg caatcaattt
    12481 cctcaaaatc aatcgcgaag tcactatcca gacggtattc cgctatgcca atcgttatcc
    12541 ggtcacgatt gaagcaattt cttcagggcg attcgatgtg aaatcgatgg tgacgcatat
    12601 ttacgattat cgggatgtac aacaggcatt tgaagagtca gttaacaaca aacgcgacat
    12661 tattaaaggc gttattaaga ttagcgatta attaatacgg gagaacagaa catgctcgca
    12721 gatatcagat attgggaaaa cgatgccaca aataaacgtt atgctattgc ccattttaat
    12781 gtatggaatg cagaaatgtt gatgggcgtt atcgacgcag ccgaagaagc gaaatcaccg
    12841 gttattattt cttttggcac aggttttgtc ggcaacacct catttgaaga tttctctcac
    12901 atgatggtat ctatggcaca aaaagcaacg gtgccggtaa taactcattg ggatcatggt
    12961 cggagtatgg agattattca taacgcctgg actcatggta tgaattcatt aatgcgtgat
    13021 gcttccgcat ttgatttcga agaaaatatt cgtttaacca aagaggctgt tgatttcttc
    13081 catccgctgg gtattccggt agaggcggaa ttagggcatg tcggtaatga aaccgtttat
    13141 gaggaagcgc tggcgggcta tcactatacc gatcctgacc aggcggctga atttgttgaa
    13201 cgtacgggct gtgactcatt ggctgtcgcc atcggcaacc agcatggggt ttatacgtca
    13261 gagccacaat tgaactttga ggtcgtcaaa cgcgtacgcg atgcagtgtc tgttccgctg
    13321 gttttgcatg gcgcatcggg gatcagtgat gccgacatta aaactgcaat ttcgttgggt
    13381 atcgcgaaaa tcaacatcca tacggagctc tgtcaggccg caatggtcgc agtgaaagaa
    13441 aaccaggatc agcctttcct gcatttagaa cgtgaagtac gtaaagctgt aaaagaacgg
    13501 gcattggaaa aaattaaact gttcggttct gatggcaaag cggaataatg atatggataa
    13561 tctcgacgtt atttgtatag gtgccgctat tgttgatatt ccattgcaac cggtcagtaa
    13621 aaatatcttt gatgtggatt cttatcctct tgaaagaatc gcaatgacca ccgggggtga
    13681 tgcaataaat gaagcaacaa ttatttctcg tctgggccat cgtacagcat taatgagccg
    13741 tattggtaaa gatgccgcag ggcagtttat tctcgaccat tgccgcaaag agaatattga
    13801 tattcaaagc ctgaagcagg acgtcaacat agatacctct attaatgttg gactggtgac
    13861 ggaggatggc gagcggacgt ttgtcacaaa ccgtaacggc agtctgtgga aattgaatat
    13921 tgacgatgtt gattttgcgc ggttttccca ggcgaaatta ttatcactgg ccagtatttt
    13981 caacagtcca ctattggatg gtaaagcgct aacagaaatt tttacgcaag ctaaagcccg
    14041 gcagatgaat atctgtgccg atatgattaa accgcggttg aatgaaacgc tggatgatat
    14101 ttgcgaagca ttgagttatg tcaattatgc cgaggcaaaa ttactcaccg ggaaagagac
    14161 actggatgaa attgctgaca gctttcttgc gtgcggcgta aaaacggtgg tgattaaaac
    14221 gggtaaagac ggctgcttta tcaagcgtgg tgacatgacg atgaaggtgc cggcggtcgc
    14281 aggaataacc gccatcgaca ccattggcgc gggcgataac tttgcttcag gttttattgc
    14341 ggcactgtta gaaggaaaaa atctgcgtga atgcgcacgc tttgccaatg caacggcggc
    14401 tatctcggtt ctaagcgtcg gtgccaccac cggcgtaaaa aacagaaagc tggtcgaaca
    14461 attgctggaa gaatacgaag gataatgaag gcaaatgaaa aagatacctt taggcacaac
    14521 ggatattacg ctttcgcgaa tggggttggg gacatgggcc attggcggcg gtcctgcatg
    14581 gaatggcgat ctcgatcggc aaatatgtat tgatacgatt cttgaagccc atcgttgcgg
    14641 cattaacctg attgatactg caccaggata taactttggc aatagtgaag ttatcgtcgg
    14701 tcaggcgtta aaaaaactgc cccgtgaaca ggttgtagta gaaaccaaat gcggcattgt
    14761 ctgggaacga aaaggaagtt tattcaacaa agttggcgat cggcagttgt ataaaaacct
    14821 ttccccggaa tctatccgcg aagaggtaga agccagcttg caacgtctgg gttttgatta
    14881 catcgatatc tacatgacgc actggcagtc ggtgccgcca ttttatacgc cgatcgctga
    14941 aactgtcgca gtgcttaatg agttaaaggc tgaagggaaa attcgcgcta taggcgctgc
    15001 taacgtcgat gctgaccata tccgcgagta tctgcaatat ggtgaactgg atattattca
    15061 ggcgaaatac agtatcctcg accgggcaat ggaaaacgaa ctgctgccgc tatgtcgtga
    15121 taatggcatt gtggttcagg tttattcccc gctagagcag ggattgttga ccggcaccat
    15181 cactcgtgat tacgttccgg gcggcgctcg ggcaaataaa gtctggttcc agcgtgaaaa
    15241 catgctgaaa gtgattgata tgcttgaaca gtggcagcca ctttgtgctc gttatcagtg
    15301 cacaattccc actctggcac tggcgtggat attaaaacag agtgatttaa tctccattct
    15361 tagtggggct actgcaccgg aacaggtacg cgaaaatgtc gcggcactga atatcaactt
    15421 atcggatgca gacgcaacat tgatgaggga aatggcagag gtcttggggc gttaaatact
    15481 caacacgatt cacctctgtt tctgtgaaaa ctgtgatgaa aatctgtgat tcaaacaggt
    15541 tattttgaaa gtaaacatcg gttatgcgat aatcgcgcta atgtgatgta aaaagataca
    15601 ggggtgtaat cgtggcggca aaagacagga ttcaggcaat taagcaaatg gttgccaacg
    15661 ataaaaaggt gacagtctca aatttgagtg ggatttttca ggtaaccgaa gaaaccattc
    15721 gccgcgatct tgagaagctg gaagatgaag gctttttgac cagaacctat ggtggtgctg
    15781 ttttaaatac agcgatgttg acggagaata tccattttta taagcgcgct tcatcgtttt
    15841 atgaagagaa gcagcttatt gcacgcaagg cactaccctt tatcgacaat aaaaccacca
    15901 tggcagccga ttccagtagt accgtaatgg aattgctcaa attgttacag gaccgtagtg
    15961 acctgacgtt gctaaccaac tcggcggaag cgattcatgt tctggctcag tcagaaatta
    16021 aagtcgtttc aacgggtggg gaactaaaca aaaatacact ttcactgcaa ggaagaatta
    16081 ctaaagagat catcagccgc tatcatgtcg atatcatggt aatgagttgt aaaggtcttg
    16141 atattaacag cggcgcgctg gaatctaatg aagcggaagc tgaaatcaaa aagacaatga
    16201 tccgtcaggc gacagaagtt gcgttattgg ttgatcactc taagtttgat cgcaaagctt
    16261 ttgtccagtt agccgacttt agtcatatta attacataat aactgataaa tcaccgggtg
    16321 cagagtggat agcattttgc aaagacaata atatccagct ggtgtggtaa tttaagcacg
    16381 cgttgtcaat attatttgtt cctgcgcctg gaaataaaat gatgataaag atgcggcaag
    16441 tgaatttgcc gcaattgcta
//
LOCUS       DBFIFI010000091        16052 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.091, whole genome shotgun sequence.
ACCESSION   DBFIFI010000091 DBFIFI010000000
VERSION     DBFIFI010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16052
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.091"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            272..1042
                     /gene="yjjP"
                     /locus_tag="N7B39_002792"
     CDS             272..1042
                     /gene="yjjP"
                     /locus_tag="N7B39_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313351.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HGU2422746.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQHPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1033..1506
                     /gene="yjjB"
                     /locus_tag="N7B39_002793"
     CDS             1033..1506
                     /gene="yjjB"
                     /locus_tag="N7B39_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HGU2422747.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLGGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1613..2152
                     /gene="dnaT"
                     /locus_tag="N7B39_002794"
     CDS             1613..2152
                     /gene="dnaT"
                     /locus_tag="N7B39_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HGU2422748.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVDLDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2155..2892
                     /gene="dnaC"
                     /locus_tag="N7B39_002795"
     CDS             2155..2892
                     /gene="dnaC"
                     /locus_tag="N7B39_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HGU2422749.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2941..3435
                     /gene="yjjA"
                     /locus_tag="N7B39_002796"
     CDS             2941..3435
                     /gene="yjjA"
                     /locus_tag="N7B39_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HGU2422750.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDVENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3689..5980
                     /gene="opgB"
                     /locus_tag="N7B39_002797"
     CDS             3689..5980
                     /gene="opgB"
                     /locus_tag="N7B39_002797"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HGU2422751.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6117..7139)
                     /gene="lgoD"
                     /locus_tag="N7B39_002798"
     CDS             complement(6117..7139)
                     /gene="lgoD"
                     /locus_tag="N7B39_002798"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HGU2422752.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITAGMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7278..8192
                     /gene="lgoR"
                     /locus_tag="N7B39_002799"
     CDS             7278..8192
                     /gene="lgoR"
                     /locus_tag="N7B39_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HGU2422753.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8407..9768
                     /gene="lgoT"
                     /locus_tag="N7B39_002800"
     CDS             8407..9768
                     /gene="lgoT"
                     /locus_tag="N7B39_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2422754.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9817..11481)
                     /gene="tsr"
                     /locus_tag="N7B39_002801"
     CDS             complement(9817..11481)
                     /gene="tsr"
                     /locus_tag="N7B39_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HGU2422755.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            11710..12252
                     /locus_tag="N7B39_002802"
     CDS             11710..12252
                     /locus_tag="N7B39_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2422756.1"
                     /translation="MLNRDFDHLSTLLHLFFEREDICQKLNIIDQNNITGWEVWFQVE
                     FANMLCSTDHEWWREQALSCDMRKKPERPTLRTDFLLRKKGWAQDSYIALEIKQNRDA
                     TSCVKNMIADLEKSAKIKRSELDLRSFWTLGITPMIDRERLDSLIDCYLDEKFYLTKS
                     RKKHVLLKEINNTPYCYIVI"
     gene            12545..14695
                     /gene="btsT"
                     /locus_tag="N7B39_002803"
     CDS             12545..14695
                     /gene="btsT"
                     /locus_tag="N7B39_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HGU2422757.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            14745..14948
                     /gene="yjiX"
                     /locus_tag="N7B39_002804"
     CDS             14745..14948
                     /gene="yjiX"
                     /locus_tag="N7B39_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HGU2422758.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            14959..15915
                     /gene="yjiA"
                     /locus_tag="N7B39_002805"
     CDS             14959..15915
                     /gene="yjiA"
                     /locus_tag="N7B39_002805"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HGU2422759.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(15984..>16052)
                     /locus_tag="N7B39_002806"
     CDS             complement(15984..>16052)
                     /locus_tag="N7B39_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HGU2422760.1"
                     /translation="GLAELKMRHEKMKTQLKKLWLV"
BASE COUNT         3874 a         4213 c         3937 g         4028 t
ORIGIN      
        1 ttaacttgtt aacactaaaa aacgcgccca gctatctatt atcctgaccc gtaaaggcta
       61 aaacaataaa ttcattatag tgataatatt catgtgatag tttgtttgcg gcgagagata
      121 attcgctttt tatcaccgag cttgtcccga taaccaggat taatcagcgc aattttgatc
      181 ttaacctacc gcaaaacaac cttgcttcat gatacaaatg gattctcatt atctgaacaa
      241 tacgcagcac gtctatgaca aagggcgagt tatgcaaact gagcaacagc gagccgtaac
      301 acggctttgt atccagtgtg gattatttct tttgcaacat ggtgcggaaa gcgcgttggt
      361 tgatgagctt tcctcacgac tgggtcgggc gctgggaatg gacagcgtcg aaagttctat
      421 ctcttcgaac gccatagtgc tgacaactat taaagatggg caatgcctga catcgacacg
      481 taaaaatcac gatcgcggca ttaatatgca tgtggtgact gaagtccagc acattgtgat
      541 tcttgcggag catcatctgc tggattacaa aggcgtagag aaacgattta gccaaattca
      601 gccattacgt tacccaagat ggctggtagc cttaatggtt ggcctttctt gcgcctgttt
      661 ctgtaaactc aataacggtg gctgggatgg tgccgtcatc accttctttg ccagtacgac
      721 cgcgatgtat atccgccagc tgctggcaca acgtcatctt catccacaga tcaacttttg
      781 ccttaccgct ttcgccgcca ccaccatttc cggattgctt ttgcaacacc ccactttcag
      841 caataccccc accattgcga tggccgccag cgttctgctg ctagtgccgg gctttccgtt
      901 gattaatgcc gtcgccgata tgtttaaagg ccacatcaat accggactgg cacgctgggc
      961 gatcgccagt ctgctgacac tggctacctg cgtcggcgta gtgatggcac tgacgatttg
     1021 ggggctacgc ggatgggtgt gatcgaattt ctgttagcgt tggcgcagga tatgatcctc
     1081 gccgccattc ctgcggtcgg ctttgcgatg gtgttcaacg ttcccgtgcg ggcgttacgc
     1141 tggtgtgcgc tgcttggctc gataggtcat ggttcccgaa tgatcttgat gaccagcggg
     1201 ttgaatattg agtggtcaac ctttatggcc tccatgctgg gcggcaccat tggtattcaa
     1261 tggtcgcgct ggtatctggc gcatccgaaa gtgtttaccg tggcggccgt tatccctatg
     1321 ttcccgggca tatcggctta taccgcaatg atttcggcgg taaaaatcag ccagttaggt
     1381 tacagcgaac cgttgatgat taccctgtta accaactttc ttacagcttc atcgattgtt
     1441 ggtgcgttat ccatcggtct ttccattcct ggattatggt tgtaccgcaa gcgccctcgc
     1501 gtataaaatt gcctcgctta agcacacgga tgagagacag cctcctctcc tccgtgtgtt
     1561 actataaaag ttatctccct tctcgttcat cgttccatat ttgagaaaca gtatgtcttc
     1621 cagagttttg accccggacg tcgttggtat tgacgccctg gtacacgatc accaaaccgt
     1681 tctggcaaaa gctgaaggcg gtgtggttgc cgtatttgct aacaatgccc cggcgtttta
     1741 tgccgtcacg cctgcacgcc tggctgaact gctggcgctg gaagaaaagc tggcgcgtcc
     1801 gggaagcgat gtcgatctgg acgatcaact ctatcaggaa ccgcaagccg ctcccgttgc
     1861 tgtacccatg gggaaattcg ccatgtatcc ggactggcaa cccgatgccg actttatccg
     1921 cctggcggct ctatggggcg tggcgctaag agagccggta accaccgaag aactggcctc
     1981 attcattgcc tactggcagg cggaaggtaa agtctttcac catgtgcagt ggcaacaaaa
     2041 actggcgcgc agcctgcaaa tcggtcgtgc cagcaacggc ggactgccga aacgagatgt
     2101 gaatacggtc agtgaacctg acagccaaat tccaccagga ttcagagggt aacgatgaaa
     2161 aacgttggcg acctgatgca acgcctgcaa aaaatgatgc ctgcccatat caaacctgca
     2221 ttcaaaacgg gtgaagagct actggcctgg cagaaagaac aaggggcgat ccgctccgcc
     2281 gctctcgaac gtgaaaatcg ggcgatgaaa atgcagcgca cctttaaccg ctccggtatt
     2341 cgcccactgc atcagaactg ctcctttgag aactatcgcg ttgagtgtga agggcagatg
     2401 aatgcgttaa gcaaagcgcg ccagtatgtc gaagagtttg acggcaacat cgccagcttc
     2461 atcttttctg gtaagccagg aaccggcaaa aaccatctgg cggcggcaat ctgcaacgag
     2521 ctgctgctac gcggtaaatc cgtattgatc atcaccgtgg ccgatattat gtcggcgatg
     2581 aaagatacct tcaggaatag cggtaccagc gaagaacaac tgcttaacga tctgagcaac
     2641 gttgatctgc tggtgatcga tgagatcggc gtgcagaccg aatcgaaata cgaaaaagtg
     2701 atcatcaacc agatcgtcga tcgccgctct tcttccaaac gcccaaccgg gatgctgacc
     2761 aacagtaata tggaagagat gaccaagttg ctgggcgaac gcgtgatgga ccgtatgcgc
     2821 ctgggtaaca gtttgtgggt gatcttcaac tgggatagct accgtagccg tgtaacaggt
     2881 aaagagtatt aatccccgtt tctggcggta tactgcgaca caattccacc ttgagtaatg
     2941 atgatgaaaa ctgttaaaca tcttctgtgc tgtgccattg ccgccagcgc attaatttcc
     3001 accggggtgc atgctgcgtc ctggaaagat gcgctctcca gcgccgccag cgaacttggc
     3061 aaccaaaaca gcacgacaca ggaaggcggt tggtcgctcg cgtcattaac taacttgctt
     3121 agcagcggaa accaggcctt aagcgcagat aacatgaaca atgccgcagg cattctgcaa
     3181 tactgcgcga agcaaaagct ggcttcggta accgatgtcg aaaacatcaa gaaccaggtg
     3241 ctggaaaagc tgggcctgaa cagtgaagag caaaaagaag acaccaacta tctggacggc
     3301 attcagggtt tgctgaaaac caaagatggt cagcaactca atctggataa catcggaaca
     3361 actccgctgg cagaaaaggt gaaaaccaaa gcctgcgatc tggtgttaaa gcaggggctg
     3421 aacttcattt cctgaaccgc gaaacgccgc gttttagctg ttacccggct gacgcggctc
     3481 tctcctgccc ttttccctgc tcctggacgg ttttccccct gttacacggg aagcgaccgc
     3541 gatcaaaaaa gtctgtttat aaactaattc ctaacaacat cgcatttttt tgacactaga
     3601 attgcagcaa tatggcgacg ccattacatt gtgcttctca aaaagcttaa acgttggcta
     3661 aacggcccgg tttagtgagg atcgcccgtt gtcagaacta ctctctttcg ccctttttct
     3721 cgcctctgtg ctgatttacg catggaaagc gggacgtaac acctggtggt ttgcagccac
     3781 attaacggtg ctggggctat ttgtcgtttt aaatatcacc ctgtttgcca gcgactattt
     3841 tactggcgat ggtattaacg acgcggttct ctatacctta accaacagcc tgaccggtgc
     3901 tggcgtcagc aaatacattt tgccgggtat cggcattgtg ctggggctga cagcggtgtt
     3961 cggtgcgctg ggctggatcc tgcgccgtcg tcgccatcat ccgcaccatt ttggttacag
     4021 cctgctggcg ctcttactgg cgctgggttc ggtggacgcc agcccggcat ttcgtcagat
     4081 aacggaactg gtgaaatccc agtcacgcga cggcgacccg gactttgcgg cttattataa
     4141 agagccgtcg aaaactatcc ctgacccgaa actcaacctg gtttatatct acggcgaaag
     4201 tctcgagcgg acctattttg ataacgaggc tttcccggac ctcacgcctg aactgggcgc
     4261 gttgaaaaat gaaggcctgg atttcagcca cacgcagcag ctgccgggaa cagattacac
     4321 gattgcgggc atggtggctt ctcagtgcgg cataccgctg tttgccccct ttgaaggcaa
     4381 cgcctccgct tctgtttcca gcttcttccc gcagaacatc tgtctgggcg atatcctgaa
     4441 aaactcgggt tatcagaact atttcgtgca gggcgcgaat ctgcgttttg ccggtaaaga
     4501 tgtgttcctg aagtcgcatg gcttcgacca cttatacggc tcagaagagc tgaaaagcgt
     4561 ggtagccgac ccgcactatc gcaacgactg gggattctac gacgataccg ttctcgatga
     4621 agcgtggaaa aagtttgaag agctttcccg ctcaggtcag cgattctcac tgtttaccct
     4681 gacagtcgat acccatcacc cggatggttt tatctctcgt acctgtaacc gcaaaaaata
     4741 tgattttgac ggtaaaccga atcagtcatt cagcgcggta agttgcagcc aggagaatat
     4801 cgcgacgttt atcaacaaaa tcaaagcgtc accgtggttt aaagatactg tcatcgtcgt
     4861 ctcttctgac catttagcga tgaacaacac ggcgtggaaa tacctcaata agcaggatcg
     4921 caataacctg ttttttgtca ttcgtggcga caagccgcag caagagacgc tggcagtgaa
     4981 gcgtaacacg atggataacg gagcgacagt gctggacatt ctcggtggcg ataactatct
     5041 cggacttggt cgtagcagtt tatccgggca gtcaatgtcg gaaatcttcc tcaatatcaa
     5101 agagaaaaca ttggcgtgga agccggatat catccgtctg tggaaattcc ctaaagagat
     5161 gaaagagttc accatcgacc agcagaaaaa catgattgcc ttctcgggta gccatttccg
     5221 tttgccgctg ctgttgcggg tttcagacaa acgcgtggaa ccgctgccag aaagcgaata
     5281 ctcagcaccg ctgcgtttcc agctggccga tttcgctcca cgcgacaatt tcgtctgggt
     5341 tgaccgttgc tacaagatgg cacaactctg ggctccggaa ctggcacttt ccaccgactg
     5401 gtgtgtctcg caggggcaac ttggcggtca gcaaattgtt cagcatgttg acaaaacaac
     5461 atggaagagc aagacggcat ttaaagatac ggtcatcgac atggcgcgtt acaaaggcaa
     5521 tgtcgatacg ctgaagattg ttgataacga tattcgctac aaagccgaca gtttcatctt
     5581 taacgtcgcc ggtgcgccag aagaggtgaa acagtttagc gggatttccc gtccggagtc
     5641 gtggggccgc tggtccaacg cgcagctggg cgatgaagta aaaatcgagt acaagcatcc
     5701 gctgccgaag aaatttgacc tggtgattac cgccaaagca tacggcaata acgccagccg
     5761 tcctattccg gtacgcgtag gcaatgaaga acaaactctt gtgctgggca atgaagtgac
     5821 caccaccaca ctgcatttcg ataacccaac cgatgccgac acactggtaa ttgtgccgcc
     5881 ggaacctgtc tcaaccaacg aagggaatat cctcggacac tcgccgcgta agctcgggat
     5941 cggcatggtg gaaattaaag tggtagaacg tgaagggtaa ttcctgactg atgtgtaggt
     6001 cgcatagggt gcgacctaca caccttctcc ggcttacaac acattattaa gaaacaaaaa
     6061 aaacagcgcc caacacttcc ccggacgctg catggcaact ttaataatct cgcctttcag
     6121 aaagtaatta cgcctttaat taactcacga ttgttaatca catcgcgctc gtaggtttct
     6181 gccagcgtgg cgaacggata gcgatgggtt aacatcatgc cagcagtgat ttttccttcc
     6241 gccatcagtc gaccgacttt agcaaaatct tccggcgtgg cgttgcggct gcccatcatc
     6301 gtcgtttctt ttttatggaa ttccggatcg gagaactgca actcaccttt aaacaggccg
     6361 acaaatacca ccgtgccgcc gtgacgaatc agattcacgg tgttattcat cgcatgttga
     6421 ttacctgtcg cgtcgatcac tttctgcgcc agcgaaccac caaactgcgc ccgcagctgc
     6481 gcgtcaaaat cttcggctga cgggtccagc acgggcaact ccagacgcgt tgccacatgt
     6541 tcacggcgcg ccggactggt atccgccacc accacctgtg cgccatcggc tttagcgatt
     6601 gccgccgcgc ccagaccgat tggcccggcc ccgaccacca gcacctgctc gccgggagca
     6661 atggctgcgc gacgcaccgc atgagcgcta atagcgaaag gttcaatcaa tgcggccgcc
     6721 tgcgggtcaa taccgtctgc gggcaaaatg ttcgccaccg gcacgctcaa atactcacta
     6781 aaaccgccat cctgatgcac gccaatgact gaaatttttt cacagcaatt ggtacgcccg
     6841 cttttacacg ccgggcattg ctgacaggca acataaggga tcacagcaac ttgctgacca
     6901 tttttaagat cagcaatatt tttacccagc ccaacaatct ccccacatat ttcatggcct
     6961 aaaacccgtg gataactaaa aaatggttga tttccacccc aggcatgaat atcggtaccg
     7021 caaatcccga cagacttaat ttttattaat gcttcattgt cacccggaat aggtatctcg
     7081 cgttgtttcc agactaattc tttcggctgc tggcaaatta aaacattcat cgtagacatg
     7141 attcggctct ccattttttg ttgccttaga tatcaatgaa aatgaaaaat ggcagcggaa
     7201 cggaaaatct gttttgtgaa aacacacgca taaaaatgtt ttaaatcggg ttttaatgga
     7261 ctcacaagga ggaaaatatg agtcgttcac aaaatttacg ccacaatgtg attaaccagg
     7321 tcattgatga tatggcgcgg ggtcatatcc cttccccact tccatcgcaa agtgcgctgg
     7381 cggaaatgta caacatcagc cgcactacag tgcgtcatat tctcagccac ttacgcgaat
     7441 gcggcgtctt aacgcaggtt ggaaacgact atgttattgc gcgtaaacct gaccatgatg
     7501 acggtttcgc ctgtactacc gcttcaatga gtgaacaaaa caaagtgttt gagcaggctt
     7561 ttttcacgat gataaaccag cgccagttac gcccagggga aactttttct gaactgcaac
     7621 tggctcgggc cgcgggtgtc agcccggtgg ttgtaagaga atatctttta aaattcggcc
     7681 gttataatct tattcaaagc gaaaagcgcg gccagtggag catgaagcaa ttcgatcaat
     7741 cctacgctga gcagctgttt gagctacgcg aaatgctcga aacacattca cttcagcact
     7801 ttctcaacct gcctgatcat gatccccgct ggctgcaggc gaaaaccatg ctcgaacgtc
     7861 atcggctact gcgtgacaac atcggcaata gctttcgcat gttctcgcaa cttgacagag
     7921 attttcactc cttactgctt tcagctgccg acaatatctt ttttgatcaa tcgcttgaga
     7981 tcatctcagt catctttcat tttcactatc aatgggacga aagcgatctt aaacagcgca
     8041 atatcattgc cgtcgatgag catatgacca ttctcagcgc tctgatttgc cgcagtgacc
     8101 ttgatgccac ccttgcgctc cgtaaccatt tgaattcagc gaaacaatca atgattcgat
     8161 ccattaatga gaacacgcgt tatgctcatt aagtacggat taaaaacagc aaactattag
     8221 cgggtgcacg gttttatcaa aagataaata ccgggaaatt cccgcttacc tatgctcaca
     8281 atcagtcgac taaaaatgtt tttgtgagcg gtagtaaagt cctaaaactt taacctgccg
     8341 ttaatgtcaa ccttgcgtaa tgcacaatta cgtaaacaat taaaattata aatacaggag
     8401 tccggcgtgg aaaaagaaaa tatcaccctc gatccgcgtt cttcatttac tccatcttcg
     8461 tcggcagata ttcccgtgcc accagatgga ttagttcaac gcagtacccg aattaaacgc
     8521 attcaaacca ccgccatgtt gttattattt tttgcggcgg taatcaatta tctcgaccgc
     8581 agttcgctgt cggtagcaaa tttaacgatt cgtgaagaat tgggattaag tgccaccgaa
     8641 atcggcgctt tgctctccgt gttttcactc gcttacggca ttgcgcaact tccttgcggc
     8701 ccactattgg atcgtaaagg cccacgcctg atgctgggac tggggatgtt cttctggtca
     8761 ctgttccagg ccatgtctgg catggtgcac agctttacgc agttcgtgtt ggtgcgtatc
     8821 ggtatgggga ttggtgaagc gccgatgaac ccatgcggtg taaaagtcat taacgactgg
     8881 ttcaacatca aagagcgcgg acgcccgatg ggcttcttca acgcagcttc taccattggc
     8941 gttgccgtaa gcccaccgat tctggcggcg atgatgctgg tgatgggctg gcgcgggatg
     9001 tttattacca ttggtgtact ggggattttt ctcgccatcg gctggtatat gctctatcgc
     9061 aaccgcgagc acgtagaact gactgccgtt gaacaagctt atctcaatgc aggtagcgtc
     9121 aatgcccgcc gagatccgct cagttttgcc gaatggcgca gcctgttccg taaccgcaca
     9181 atgtggggaa tgatgctcgg attcagtggc atcaactaca ctgcgtggct gtatctggcc
     9241 tggcttcctg gttacctgca aacagcctat aacctggatt taaaaagcac agggttgatg
     9301 gcggctatcc ctttcctgtt tggtgctgcc gggatgctgg tcaacggtta cgttactgac
     9361 tggctggtca aagggggaat ggctccgatt aaaagccgta agatctgcat tattgccggg
     9421 atgttctgtt ctgccgcctt tacgctgata gtaccacaag cgacaacatc catgatggcg
     9481 gttctgctga ttggcatggc actgttctgt attcactttg ccggaacatc ctgctggggc
     9541 ttgatccacg tcgcagttgc ttctcgcatg actgcgtcgg tgggcagtat ccagaacttt
     9601 gccagcttca tctgcgcctc tttcgcgccg atcattactg gttttattgt tgatacaacc
     9661 cactcattcc gtctggcact aatcatttgc ggttgcgtca ccgcagcggg agcactggcg
     9721 tacatcttcc tggttcgtca gccgatcaac gacccgcgta aagattaaac tccctatgcc
     9781 cgataacatt ttgcttatcg ggcattttta tggcgattaa aacgtttccc agttctcccc
     9841 gctatctgcc actgccattt tacgcggcgt agctggcgtc acggttttta ccacagccga
     9901 tgtttcacgc tgctgttgct gctgttgctg aatccggaac actgccacag cttcggtcag
     9961 gcgactggcc tgctcttcca acgcggcggc ggcagcggca gactcttcca ccagcgcagc
    10021 gttctgttga gttacccggt ccatctcagc aaccgctaag ccaacctgat cgataccacg
    10081 gctctgctca tcagaagcag aagctatttc gcccataatg tccgtcacgc gggtcacagc
    10141 actgacaatc tccgccattg tttccccggc gctttcgacc agcgtagagc caacatccac
    10201 tttccccacc gagtcttcaa tcaggctttt aatttcacga gccgcctggg cgctacgctg
    10261 ggccagatta cgcacttctc ccgcaaccac cgcaaaacca cgtccttgct cacccgcacg
    10321 cgctgcttca accgccgcgt tcaaagccag aatattggtc tggaaggcaa tgccgtcaat
    10381 tacgctgata atatcggcga ttttctgcga actggtggag atatcacgca tagtctgcac
    10441 cacgttatct accactttgc cgccgcgttg cgccgtttca gaagcactta acgccaggtg
    10501 gctggcctgg cgcgcattct cggcgttctg tttcaccgtt gcggtgagtt gctccatgct
    10561 ggctgccgtc tcttccagcg aagcggcctg ttgctcggtg cgcgaagaga gatcgttatt
    10621 gccggtagcg atttcgctgg caccgctata gatggcattg gccccgttgc gcacatcacc
    10681 gacggtacgc atcagctctc cctgcatatg gcgcaaactc tctgccagtt gccccatctc
    10741 attagagcca tccacctcaa tcggtttcac cagatcgccg cctgcaatat gacgaatgct
    10801 gtcaatcagg cgattcattg gcgctaccag cgaggcttta ataccgaacc agacggcgaa
    10861 gatgaccgcc agtacgacga tcatcacgcc caccagaatc cacatcgcct ggctgtagga
    10921 ggcattgtta tcgctgacgg cgatatcata gagccgatcg ttttgctcca tgtaagccac
    10981 atactgcttc tcgaaaccgt cctgatatcc ctgggtcggc tgatcaaaga actcgttgat
    11041 tttgcctgca cctaacagtt ggatcagctc cgccagcgca ttgtgataaa tatcgtaatt
    11101 acgtttgatc tctgccgctg cggcggtgct ctgacgcggg tcacgcggca acgcttcgta
    11161 atctgcccag tttttttccg cctgtttcaa cgaaatactg gcactctgca tcagctcagc
    11221 aacggttgaa ccgctaccaa tattattctg atccatcatg tagcggatac ccgcgcggtt
    11281 gagggtgtta cgcgtctgca acaacgcgac ccagctgcca ttcagcgtgg attgctgctg
    11341 gcgaatggtt tgtaaaacag tgaaattttc tttgtcattc tttaaggcat taaagaacag
    11401 accgcctgat gtcagttgta aaaggccaaa aacggccaaa accagcagta agctggtcac
    11461 gatcttgata cgttttaaca tggtttctct ttcctgtgga ccgatgcaaa attttcggcc
    11521 tgaaatgaga aaactttata ggtagcgact tgaaactggt taattttacc tcactttttt
    11581 taatttttgt tcaaaaatac aataaccact tattgataca ttttcttttc ttattttttt
    11641 cacacgaatt gtatctattt tgaacgaaat attttataga gtgtggaatt ttaagaaagg
    11701 acatctgaaa tgttaaaccg tgacttcgat catctctcaa cgctattaca tctctttttt
    11761 gaacgtgagg atatttgcca aaagcttaat attattgacc aaaacaatat caccggttgg
    11821 gaagtgtggt tccaggtaga gtttgctaat atgctctgct caacagacca tgaatggtgg
    11881 cgtgaacaag cactttcctg tgatatgcgc aaaaaacctg agcgcccgac actacgcaca
    11941 gatttcttac tacgtaaaaa aggctgggca caagatagct atatcgcgct tgaaataaaa
    12001 caaaacagag acgcgaccag ttgtgttaaa aatatgatcg cagacttaga aaaaagcgca
    12061 aaaataaaac gttccgaact cgatttacgc agtttctgga ccttgggtat cacccctatg
    12121 atagacaggg aaaggctgga ctctctcatc gattgctatt tagatgagaa attttatctt
    12181 actaaatctc gtaaaaaaca cgttttactt aaagaaataa ataacacacc atattgctat
    12241 attgttattt aatatttata gtatgaatat aatttttaaa tgagttacac aaccgagggc
    12301 taaaaatcgc ttcggttgtg atcatcatca cataaataac cttcataaac cttgctcaaa
    12361 accgctcaac cgcaaaactg accgcttacg tccctaaaat aaccactcag ttatttacct
    12421 tactttacgc gcgcgtaact ctggcagcat cattgcagga tagcggtcaa tttacctcct
    12481 caaacgcaac gcaaacctag aacggcttcg gccaactatt aatcaataca tgccaggttt
    12541 tactatggat acgaaaaaac tattcaagca cataccctgg gtgattctcg gaatcatcgg
    12601 tgcattctgc ctcgcggtag ttgcattacg tcggggggag cacgtcagcg ccctgtggat
    12661 cgtggtcgcc tctgtgtcgg tgtatctggt ggcgtatcgc tactacagtc tgtacatcgc
    12721 ccagaaggtg atgaaactcg accccacgcg cgcgacgcct gcggttatta acaacgacgg
    12781 tctgaactac gttccgacca accgttacgt gttgtttggt caccacttcg ccgctatcgc
    12841 cggtgctggt ccgctagtgg gaccggttct cgccgcgcag atgggctacc tgcctggcac
    12901 gctgtggctg ctggcggggg tagtgctggc cggtgcggtt caggacttta tggtgctgtt
    12961 tatctcctct cgccgtaacg gcgcatctct cggtgagatg atcaaagaag agatggggcc
    13021 agtaccgggg actatcgcgc tgtttggctg tttcttaatc atgatcatca tcctcgccgt
    13081 cctggcgctg attgtggtta aagccctggc agaaagtccg tggggtgtct tcaccgtttg
    13141 ctcaaccgta ccaattgcgc tgtttatggg tatctacatg cgctttatcc gtccggggcg
    13201 tgtgggtgaa gtctctgtta ttggtatcgt gctgctggtt gcctctatct acttcggtgg
    13261 cgtgattgct cacgatccgt actggggtcc ggcactgacc tttaaagaca ccaccattac
    13321 cttcgcgctg attggctatg cgtttgtttc cgcgctgctg ccggtgtggc tgatcctcgc
    13381 accacgcgac tatctggcaa ccttcctgaa gatcggcgtt atcgtcggcc tggcgctggg
    13441 tatcgtggtg ctgaacccgg aactgaaaat gcctgcgatg acccagtaca ttgacggtac
    13501 tggcccgctg tggaaaggcg ctctgttccc gttcctgttc atcaccatcg cctgtggtgc
    13561 ggtatctggc ttccacgcgc tgatctcttc cggtacgacg ccgaaactgc tggctaacga
    13621 aaccgacgcg cgtttcatcg gctacggcgc aatgctgatg gagtccttcg tggcgattat
    13681 ggcgctggtt gctgcatcca tcatcgaacc gggtctttac ttcgcgatga acaccccgcc
    13741 cgcgggcctt ggcatcacca tgcctaacct gcatgaaatg ggtggcgaga acgcgccgat
    13801 catcatggcg cagctgaaag acgttaccgc acacgcggca gcgaccgtca gctcttgggg
    13861 cttcgtgatt tcgccagagc agatcctgca aaccgcgaaa gacattggtg agccttctgt
    13921 cctgaaccgt gcaggtggtg cgccgacgct ggcggtaggt atcgctcatg tgttccacaa
    13981 agtgctgccg atggctgaca tgggcttctg gtatcacttc ggtattctgt tcgaagccct
    14041 gttcatcctg accgcgctgg atgcgggtac ccgttctggc cgctttatgc tgcaagacct
    14101 gctgggtaac ttcatcccgt tcctgaaaaa aaccgactct ctggttgctg gtatcatcgg
    14161 tactgcgggc tgtgtgggtc tgtggggcta cctgctgtat cagggcgtgg tcgatccgct
    14221 gggcggcgtt aagagcctgt ggccgctgtt cggtatctcc aaccagatgc tggcagccgt
    14281 agcgctggta ctgggcaccg ttgtgctgat taagatgaag cgcacccaat acatctgggt
    14341 aactgttgtt ccggctgtat ggctgcttat ctgcaccacc tgggcgctgg gtctgaaact
    14401 gttcagcacc aacccgcaga tggaaggctt cttctacatg gcaagccagt acaaagagaa
    14461 gattgctaac ggtactgacc ttacggcgca gcagattgcc aacatgaacc acatcgttgt
    14521 gaacaactac accaacgcag gcctgagtat tctgttcctg attgtggtgt acagcatcat
    14581 cttctacggt ttcaaaacct ggcttgcggt acgtaacagc gacaaacgta ctgacaaaga
    14641 aacaccgtac gttccaatcc cggaaggcgg cgtgaagatc tcttcgcacc actaaccgtg
    14701 tttagccccg cttcggcggg gctttgttct tatcagagtg aactatgttt ggtaacttag
    14761 gacaggcaaa aaaatatctc ggccaggcgg cgaagatgtt gattggcatt ccagactacg
    14821 acaactacgt cgagcatatg aagaccaacc atcccgacaa gccgtacatg agctatgaag
    14881 aattcttccg cgaacgccag aacgcgcgct acggcggcga tggtaaaggc ggtatgcgct
    14941 gttgttaatg gagagagcat gaacccgatt gcagttaccc tactcaccgg ttttttaggc
    15001 gcgggcaaaa ccaccctgct gcgccatatt cttaacgaac aacacggcta caagattgcc
    15061 gtgattgaaa acgaatttgg cgaagtctcc gttgatgatc aactgattgg cgaccgcgcc
    15121 acgcagatca aaacgctgac caacggctgc atctgctgtt cgcgctccaa cgagctggaa
    15181 gacgcactac tcgacctgct ggataatctc gataagggca atattcagtt cgatcgtctg
    15241 gtcattgaat gcaccggcat ggccgatccc ggcccgatta ttcagacctt tttctcccat
    15301 gagattttat gccagcgtta cctgctggac ggcgtgattg cgctggtgga tgcggtacat
    15361 gccgatgagc agatgaacca gttcaccatc gcccagtccc aggttggcta cgccgaccgt
    15421 attctgctga ccaaaaccga cgtcgcaggc gaagcagaaa aactgcgcga acgcctggcg
    15481 cgcatcaacg cccgtgcacc ggtctacacc gttacccacg gcgacatcga cctgggcctg
    15541 ctgttcaaca ccaacggttt tatgctggaa gaaaacgtcg tcagcaccaa accgcgtttc
    15601 cactttatcg cggataaaca aaacgatatt tcgtcgattg tggtagaact ggattacccg
    15661 gtagatatca gcgaagtttc ccgcgtgatg gaaaacctgc tgctggaatc ggcggataaa
    15721 ctgctgcgtt acaaagggat gctgtggatt gacggcgaac ctaaccgcct gctgttccag
    15781 ggcgtccagc gcctctacag cgccgactgg gaccgcccgt ggggcgatga aaaaccgcat
    15841 agcacaatgg tttttatcgg gattcagttg ccggaggaag agattcgggc agcgtttgcg
    15901 gggttgagga agtaagttcg ggaaagatca actttttgag agttccaccg caacattatt
    15961 acgagtggaa ctctttagat atgtcagacc aaccaaagct tcttcaattg cgttttcatt
    16021 ttttcatgtc tcattttcag ttcagccaat cc
//
LOCUS       DBFIFI010000092        16050 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.092, whole genome shotgun sequence.
ACCESSION   DBFIFI010000092 DBFIFI010000000
VERSION     DBFIFI010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16050
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.092"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(358..783)
                     /gene="arsC"
                     /locus_tag="N7B39_002807"
     CDS             complement(358..783)
                     /gene="arsC"
                     /locus_tag="N7B39_002807"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HGU2422761.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTTLG
                     TRLCRPSEVVLEILPDAQKGAFTKEDGEKVVDEAGKRLK"
     gene            complement(796..2085)
                     /gene="arsB"
                     /locus_tag="N7B39_002808"
     CDS             complement(796..2085)
                     /gene="arsB"
                     /locus_tag="N7B39_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HGU2422762.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(2139..2492)
                     /gene="arsR"
                     /locus_tag="N7B39_002809"
     CDS             complement(2139..2492)
                     /gene="arsR"
                     /locus_tag="N7B39_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HGU2422763.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPSWAVKIIEQAWRCEQEKVQVIVRN
                     LARQNCSVDSKNTCS"
     gene            3236..3319
                     /gene="dinQ"
                     /locus_tag="N7B39_002810"
     CDS             3236..3319
                     /gene="dinQ"
                     /locus_tag="N7B39_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HGU2422764.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(3373..4725)
                     /gene="gorA"
                     /locus_tag="N7B39_002811"
     CDS             complement(3373..4725)
                     /gene="gorA"
                     /locus_tag="N7B39_002811"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HGU2422765.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNVEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(4797..5639)
                     /gene="rlmJ"
                     /locus_tag="N7B39_002812"
     CDS             complement(4797..5639)
                     /gene="rlmJ"
                     /locus_tag="N7B39_002812"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HGU2422766.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEPYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKSDGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            5842..7884
                     /gene="prlC"
                     /locus_tag="N7B39_002813"
     CDS             5842..7884
                     /gene="prlC"
                     /locus_tag="N7B39_002813"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HGU2422767.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQVLREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            7892..8644
                     /gene="rsmJ"
                     /locus_tag="N7B39_002814"
     CDS             7892..8644
                     /gene="rsmJ"
                     /locus_tag="N7B39_002814"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HGU2422768.1"
                     /translation="MKICLIDETGTGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(8693..10162)
                     /gene="dtpB"
                     /locus_tag="N7B39_002815"
     CDS             complement(8693..10162)
                     /gene="dtpB"
                     /locus_tag="N7B39_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HGU2422769.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGTFTLFYMSINIGSLIAL
                     SLAPVIADKFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            10328..10381
                     /locus_tag="N7B39_002816"
     CDS             10328..10381
                     /locus_tag="N7B39_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2422770.1"
                     /translation="MVLNIVLAGDFDCERRP"
     gene            10359..10412
                     /locus_tag="N7B39_002817"
     CDS             10359..10412
                     /locus_tag="N7B39_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2422771.1"
                     /translation="MIANADRRPDKAFTLIR"
     gene            complement(10479..10913)
                     /gene="uspA"
                     /locus_tag="N7B39_002818"
     CDS             complement(10479..10913)
                     /gene="uspA"
                     /locus_tag="N7B39_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HGU2422772.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            11304..11639
                     /gene="uspB"
                     /locus_tag="N7B39_002819"
     CDS             11304..11639
                     /gene="uspB"
                     /locus_tag="N7B39_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HGU2422773.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(11710..13209)
                     /gene="pitA"
                     /locus_tag="N7B39_002820"
     CDS             complement(11710..13209)
                     /gene="pitA"
                     /locus_tag="N7B39_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HGU2422774.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            13441..14643
                     /gene="yhiN"
                     /locus_tag="N7B39_002821"
     CDS             13441..14643
                     /gene="yhiN"
                     /locus_tag="N7B39_002821"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HGU2422775.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTVRLRSEVLSVAKDDTGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSC"
     gene            complement(14961..16013)
                     /gene="yhiM"
                     /locus_tag="N7B39_002822"
     CDS             complement(14961..16013)
                     /gene="yhiM"
                     /locus_tag="N7B39_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HGU2422776.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
BASE COUNT         4131 a         4059 c         4180 g         3680 t
ORIGIN      
        1 gtgatgtttt agatgtgtaa taataaaatg ggttaaggta tcttataatt attgaataat
       61 ttgttgtgta aagcactgat aaatcataat ccagtacgtt tttaaactgc tgcactgtat
      121 cgctgatcac taagcagctc actgatacat catgctggac gcgataattg gaatctcacg
      181 cagggagtaa tgtgtaaata gcaaaatcgc ctgatgcgct acgcctgtca ggcctacaaa
      241 taacatacaa tttattgaat tttcatgact ttgtagatcg gataagacgc gttagcgtcg
      301 catccgacat aaataaagcg cacttttcta acaacctgtg ggggggatat agccgcttta
      361 tttcaggcgc ttacccgctt catcaaccac tttctcgcca tcttccttgg tgaatgcgcc
      421 tttttgcgca tctggcagaa tttccagcac cacttctgaa gggcggcaca ggcgagttcc
      481 cagcgtcgtc accacaatcg ggcgattaat cagaatcggg tgctgaagca taaagtcgat
      541 taaccgatcg tcagtaaatt tatcttccgc aaggcccagc tcctcatacg gttcgacgtt
      601 tttacgcagc agcgcgcgta cggaaatccc catatcggca atgagtttga ccagttcatc
      661 gcgcgttggc ggagtttcca gataatggat aatggtcggt tctgtgccgc tgttgcggat
      721 catctccagc gtattacgcg acgtgccgca ggccgggttg tgataaatgg taatgttgct
      781 catatcagta tctcattaca aagtgaaaga gagacgtagc gccagcgcag ccagcgtcac
      841 aaacagcaca ggcagggtca tgataatccc tgtacggaag taatatcccc agctgatagt
      901 catattcttc tgcgaaagta cgtgcagcca gagcagcgtt gccaggctac caatcggggt
      961 gattttcggc cccaaatcgc agccaatcac gttggcataa accatcgctt ctttgataac
     1021 gccagatgcc gtgctgccat caatggacaa cgcgccaacc agtaccgtcg gcatattgtt
     1081 cataatagaa gagaggaagg cggtgaggaa tccggtgccg agcgtcgcgg cccacaggcc
     1141 gttatccgcc agcacgttga gtacgccaga aagatattcc gttaatccgg cattgcgcag
     1201 gccataaacc accagataca tgccgagcga gaagatgaca atctgccagg gcgcaccgcg
     1261 cagcacttta cccgtattaa tcgcatgacc gcgtttagcg acgacaaata atatcaacgc
     1321 gcccactgcc gcaatggcgc tgaccggaat gccgagcggt tccaggacga aaaatcccac
     1381 cagcagaagc agtaaaacaa cccagccagt tttgaacgta gcaggatctt taatcgcttc
     1441 tgcgggagat ttcagcagcg ccatgtcgta gttctgcgga atatctttgc ggaaatagag
     1501 atgcaacatc accagcgtgg caacaatcgc ggcgatatcc accggcacca tcaccgaggc
     1561 atattcgcga aagccgaggc caaagaaatc tgcggaaacg atattcacca ggttggagac
     1621 aataagcggc aggctggcgg tatcggcaat gaatccggcc gccatcacaa acgccagcgt
     1681 agtgccttta ctgaacccta aagccagcag catggcgatg acaatcggtg tcaaaataag
     1741 cgccgcgcca tcattggcaa acagggcggc aacggcagca ccgagcagga caatccaggt
     1801 aaacagcaag cgaccgcgtc cattgcccca gcgtgaaacg tgcagtgccg cccattcaaa
     1861 aaagccggac tcatccagca gcaggctgat gataataacg gcaataaacg cagccgtcgc
     1921 gttccagacg atattccaca ccaccggaat atcgcccgga tgtactacgc ccgtaactaa
     1981 cgccagtact gcgccgagcg ttgcactcca gccgatgcct aaacctttcg gctgccagat
     2041 aaccaataca atggtcagga caaagatagc gcctgccagt aacataatgc ctcccggaaa
     2101 aacacatgtg caaatccata tgtgtttaga taaatttttt aactacaagt gttcttactg
     2161 tctacggaac aattttgtct ggccaggttg cggacaatca cctgaacctt ttcctgttcg
     2221 catcgccagg cctgctcaat aattttcacg gcccatgatg gaatatgcgg tgataagcgg
     2281 taatgaaccc acttaccctg cttgcgatcc agcaacaaac cgctttcacg aagaagcgcc
     2341 agatggcggg agatcttggg ctgcgactgg tcgagagcag tgcagagatc acagacgcat
     2401 aactctccca gctcgctgag cagtaaaacg atgcccagac gggtttcatc ggcaagaatt
     2461 ttgaacagtt ggatgggtaa cagaaatgac atattgcgct cctgattgtt gcaggtagtg
     2521 tctctcttcg aagcggataa gtcaaaaaca tatatggttt aacgaatgtg tatgtgcaga
     2581 ggaaggtaat aggtgtgaat taagcgttgg ctattcattt gaaaggaggt ctgaatcagg
     2641 acgtaaaaaa accgactttg cgtcggtttt ttactttcca gcccagagtt ggtggctctg
     2701 gctggagtga gaatgcccac atctaacggt tcaatactat tagccagtgg ctaacattgc
     2761 aagcgttagc gatttcttca gctggagaag ggacagtttg ctatacatgt tggtgaaaga
     2821 ggatttatat actttcatcg ggtcgctgcc gtaccaggct ttgtccgaat ccggatgtaa
     2881 aaaaaccgac ttcgcgtcgg tttttttact cttcagccct gagttggtgg ctctggctgg
     2941 agtgagaatg ccatcatcta acgcatcaat actaatacca tactgaaact attgcaagga
     3001 catgctggtt ttataacctg aatatactgt atgattatcc agttagctct gaggcatttt
     3061 cactctggca atgcgcataa acgctttcaa agtcctgttc agtggtacgg gtggtgccgt
     3121 taactgatgc tctggccgga gtgagaatac cattatctaa caatagggca tgcgccgtga
     3181 caggcgatgg atgagtaagc ggatgcattc tcactccatc gtatggagaa cacgggtgat
     3241 tgataaagca atcatcgttc ttggggcgtt aattgcgctg ctggaactga tccgctttct
     3301 gcttcagctt ctgaactgat agcggaaacg taattaaggg caaagagcac accactcttt
     3361 gccctttaac atttaacgca tggtcacgaa ctcttctgcc gccgttgggt gaatggcgac
     3421 ggtattatcg aagtcttttt tggttgcgcc catcttcagt gccaccgcga agccctgcaa
     3481 catttcgtcc ataccaaaac caatgccgtg aataccgaca atcttctctt ctggcccaac
     3541 gcacaccagc ttcatgcggc acggctggcg gtgagtggtg acggcggtat acatcgcggt
     3601 gaaagaggat ttatacactt tcacctgatc gtcgccgtac tgctcgcgcg cctgcggttc
     3661 cgttaaacca acagtaccaa tcggcggatg gctgaagacc acggtcggaa tgttgctgta
     3721 atccagatgc tcatccggct tgttattaaa caggcgttca gagagacggc gacccgctgc
     3781 aactgccacc ggtgtcagtt ctactgcacc cgtgttatcg cccaccgcgt aaataccttc
     3841 aacgttggtg ttttgatatt tatcgacgac gatatagcct ttttcgttgg ttttaacgcc
     3901 agcggcttcg aggttgatgt tgtcattggc aggctcgcgg ccaatcgccc aaatcaggca
     3961 atccaccgtt tcactgcgac catcttccag ctccagcgtc aggctaccat cggcattttt
     4021 cactaccgct ttcgggatgg cgttggtgtg caactgcggg ccttcggcgt tcatcacttc
     4081 gaccagcgtt tcagagatca tcgggtcgaa gctgcgcagc ggcgcatgtt tacgcacaaa
     4141 cagatgcgtt ttcgcgccga ggccgttaat cacgccagcc agctcaacgg cgatgtaacc
     4201 cgcgccaaca accgccacac gctctggcaa agcaggaagg gcaaagaaac catcagaatc
     4261 aataccgtat tccacgcccg gaatatccgg gtggctcgga cgaccgccgg tggcgatcag
     4321 aatatgatcg gctgtgatgg tttcaccgtt aacttccagc gttttggcat caacgaagcg
     4381 ggcaaagcct ttgattacat caacgttatt tttaccgagc acgttttcat aggaagtatg
     4441 aatacggtcg atataggcgg tacggctggc gatcaacgtt tcccagttga atttattgat
     4501 agtggtatca aaaccataat ccgggccgta catatggatc gcttcacgga tttgcgccgc
     4561 gtgccacatc actttcttcg gtacgcagcc gacgttcacg caggtgccgc ccagctcttt
     4621 ggcttcaatc agcgcgcatt tctgaccgta catagccgcg cggttgatgg aggcgatacc
     4681 gccgctgccg ccgccgatgg cgatgtaatc atagtgttta gtcatgacag agtgtcctta
     4741 tcgttgatta ccgcgattgt agcgcgagac gtaataggtg ccagcaatgg ctgcaattac
     4801 tctggcacga tccagcttac ggtggcgtgc ccggtgccag ctggaaccag tttgctgtgc
     4861 agccacggca gcacgttatt catctgctgt tccagtttcc acggcgggtt aatcacaatc
     4921 atgccggaag cggtcatgcc acggcgatcg ctgtctggca gtaccgccag ttcaatttgc
     4981 agaattttgc gaatgccggt cgcttccagg tcgtggatca tgcgcttaat ttgctggcgc
     5041 agcaccaccg gataccacaa tgcgtaagtg ccagtggcga aacgtttgta accttctgct
     5101 atcccgctga ctaccgcctg atagtcggtt ttcatttcat acggcggatc gataaggatt
     5161 aaaccacggc gagaaaccgg cggcagtttg gccttaagct gctggaaacc gtcggatttt
     5221 tcgacgcgcg cacggctgtc tttctgaaat tcagaacgca gcaacgggta atcgctcggg
     5281 tgcagttcgg tcagttgcag gctgtcctgt tcacgcagta actgacgggc aatcaacggt
     5341 gaaccggggt agtaacgcaa ctgaccgcta cggttgaagt gttttaccac attgatgtac
     5401 ggctccagct ccgcaggcaa atcgtcctgc tgccagatac gggcgatacc ttcgagatat
     5461 tcgccggtac gctcggcatg ttcgctgcct aactgataac gcccggcccc tgcgtgggtg
     5521 tcgagataga gaaacggttt atctttctct ttcagcgact cgatgatcag gctctgaacg
     5581 gtatgtttaa ggacgtcggc gtggttgcca gcgtgaaagc tgtggcgata actgagcatg
     5641 ggtaaaggtg ttccggtaag taaaaatcgg cccgtattcg gggcgcacaa aagcctatca
     5701 ggacagtata ccgaaaagag gccgccgccg cgaaagcgta acgtttctca ttgaaattca
     5761 ctacacttaa ccccatgcta caaacattat gtaaagcgcc tgttgagcgc ttccttaacc
     5821 tctttaacca ggactgcgct aatgacgaat ccgttactga ctccctttga attgcctccg
     5881 ttttctaaaa ttctcccgga acatgtcgtt ccagccgtga ctaaggcatt gaatgactgc
     5941 cgcgaaaatg tggagcgcgt agtagcgcaa ggagcaccgt acacctggga aaatctctgc
     6001 cagccgttgg cggaagtgga cgatgtgttg gggcgtattt tctccccggt cagccacctg
     6061 aactcggtga aaaatagccc ggaattgcgc gaagcgtacg aacaaactct gccgctgctg
     6121 tcggaataca gcacctgggt agggcaacat gaagggctgt ataaagcgta ccgcgacctg
     6181 cgcgatggcg atcattacgc cacgctgaac acggctcaga aaaaagcggt tgataacgca
     6241 ctgcgcgatt ttgaactctc aggcatcggt ctgccgaaag agaaacaaca gcgttacggc
     6301 gaaattgcca ctcgtctttc tgaactgggc aaccagtaca gcaacaacgt ccttgatgcg
     6361 acgatgggct ggactaaact cgttaccgac gaagcggaac tggcggggat gccagaaagc
     6421 gcgctggctg cggcaaaagc ccaggccgaa gcgaaagagc tggaaggcta cctgttgacg
     6481 ctggatatcc caagctactt gccggtaatg acctactgcg acaaccaggt cttgcgtgaa
     6541 gagatgtatc gtgcttacag cacccgcgct tcagatcaag gtccaaacgc cggtaaatgg
     6601 gataacagta aggtgatgga agagatcctc gcgctgcgtc acgaactggc gcaactgctg
     6661 ggctttgaaa actacgcgtt taaatcactg gctaccaaaa tggcggaaaa cccgcagcag
     6721 gtgcttgatt tcttaaccga tctggcaaaa cgcgcgcgtc cgcagggcga aaaagagctg
     6781 gcgcaactgc gtgccttcgc caaagccgaa tttggcgtgg atgagttgca accgtgggat
     6841 atcgcatact acagcgaaaa acaaaaacag cacctctaca gcatcagcga cgaacaactg
     6901 cgtccgtact tcccggaaaa caaagcggtt aacggcttgt ttgaagtggt aaaacgtatt
     6961 tacggcatca ccgctaaaga gcgtaaagat gttgatgtct ggcacccgga tgtacgtttc
     7021 ttcgaactgt atgacgagaa caacgaactg cgcggcagtt tctacctcga cctgtatgcc
     7081 cgtgaaaaca agcgcggcgg ggcgtggatg gatgactgcg taggccagat gcgtaaagcc
     7141 gatggttcgc tgcaaaaacc ggtcgcgtat ctgacctgta acttcaaccg cccggtgaat
     7201 ggtaaaccgg cgctgttcac tcacgacgaa gtgatcactc tgttccacga gttcggtcac
     7261 ggcctgcacc atatgctgac ccgcatcgaa accgccggtg tttccggtat cagcggtgtg
     7321 ccgtgggatg cggtcgaact gccgagtcag tttatggaaa actggtgctg ggagccggag
     7381 gcgctggcgt ttatctctgg tcactacgaa accggcgaac cgctgccgaa agagttgctg
     7441 gataaaatgc tggcggcgaa gaactaccag gcggcgctgt ttattctgcg ccagctggag
     7501 ttcggcctgt tcgatttccg cctccatgcc gagttccgcc cggatcaggg agcgaaaatc
     7561 ctcgaaactc tggcagaaat caagaaactg gttgccgtag taccgtctcc atcctggggc
     7621 cgtttcccgc acgctttcag ccatattttc gccggtggtt atgccgcagg ttactacagc
     7681 tacctgtggg ctgacgtact ggcggcagat gctttctcgc gctttgagga agagggcatt
     7741 ttcaaccgtg aaaccgggca gtcgttcctc gacaacattc tgagccgtgg cggttcagaa
     7801 gagccgatgg atctgttcaa acgcttccgt ggtcgtgaac cgcagctgga tgcgatgctg
     7861 gagcattacg gcattaaggg ctgatcattc agtgaaaatc tgcttaattg atgaaacagg
     7921 caccggagac ggtgccttat ctgttctggc ggcccgctgg gggctggagc acgatgaaga
     7981 caacctgatg gcgctggtgt taacgccgga acatctggaa ttgcgcaagc gtgatgagcc
     8041 aaaacttggc ggcatctttg ttgattttgt tggcggagcg atggcgcacc gacgcaaatt
     8101 cggcggtggt cgcggtgagg cggtggcgaa agcggtgggc attaaaggcg attatttgcc
     8161 ggatgtggtg gacgctaccg cagggctggg gcgcgatgcc tttgtgctgg cttcggtcgg
     8221 ctgccgcgtg cggatgctgg agcgtaatcc agtcgttgcc gcgctactcg acgacggcct
     8281 ggcgcgtgga tatgcggatg cggaaatcgg cggctggttg caggagcgtt tgcagttaat
     8341 tcacgcctcc agcctgacgg cgctgaccga tattaccccg cgtccgcagg tggtctatct
     8401 cgacccgatg ttcccgcata agcagaaaag cgcgctggtg aaaaaagaga tgcgcgtctt
     8461 tcagtcgctg gtgggaccgg atcttgatgc cgatggatta ctggagcctg cacgcttgct
     8521 ggcgaccaaa cgcgtggtgg taaagcgccc ggactacgcg ccaccgctag cgaatgtcgc
     8581 cacgccaaac gcggttgtga ctaaagggca tcgctttgat atctatgcag gtacgccagt
     8641 gtaaaagtaa agcagggtag cgataacgct accctgcttt ctgcaagaat aattaatggc
     8701 tttccggcgt cgcaatcatg cgtttcagcc acggcaccat caacagcatc actactgcaa
     8761 cgcccagcgt gaccagacca atcttaccaa acacgttggt atagacaggc aacgtctcaa
     8821 gcggatcggt aatgttgtct ggtactgcgg taaatgttgc cacatagccg cccagcaaga
     8881 acgcggcagc ctgtgtcagg aaccacatcc cgagaataaa gcccatcaaa tgctgcggca
     8941 ccagagcagc aatcatcgcc aggccaaggg cgctaataaa cagttcacct aagctctgga
     9001 ataagtacac cagcacgata aaccatggcg atgtcagccc ttgtgcatcc gcaaaccaca
     9061 ttcccgcagc tgccgccgtc aaaaagccca gtgagcacat aaacatgccg agagtaaatt
     9121 tcatcggcat cgagaggtct ttgcctttgt tacccagatg cgtgtaaatg cctgccagta
     9181 ttgggctggc gagtaccacc cagaacgggt taagcgcctg gaagctgacc gggttgatgg
     9241 aaaaaccgag aatttcatga tgcacgttgt tgatggcaaa gaagttcagc gatgttggca
     9301 tctgggcgta gagaatgtaa aacaccaccg cttcgagcat caggacaaag gcgacaaaca
     9361 ttttattgcg cccggtttta tccagcttga atgcctgacg aaagaagatg atggtgacga
     9421 cgatggagag aacaatcagc accagattgg cgacttctac gttgtgcatc agccaggcgc
     9481 agacgaagat catcaccacg ctgccaagta atacgtacaa cagtttgctg aagctcattg
     9541 gccggaagtc gggctcagaa ccaatgtctt tcaccattcc acgacaggcg atgtaaacca
     9601 gtaatgcgat aattaacccc gcaccgcaca ggttgtaggt gactgaatag ccgaatttat
     9661 cagcgatcac aggggccagc gataacgcta tcaacgagcc gatattgatc gacatataga
     9721 acagggtgaa tgtgccatca aggcgcggat ctttcggcgg atagcacttc gaaagcaagc
     9781 tggctgggtt agctttaaac aggccgttac cgacagcgat agtccccagg gcgatgaaaa
     9841 tcaggtcagg cttaagtagc gacatgccgg tcatgaagta gccaatcgcc agcacaagtg
     9901 ctccaagaac gatggtgcgt ttggtcccca gcaggtggtc gccgacatag ccgccaatgg
     9961 aaatgaggcc atagaccagt gcagcaaaag caccaaaagt gacaaaagcc tgctcttgcg
    10021 agaatccaag ctgtttaacg aagaaaaccg ccagtacgcc ctgcacgccg tagtagccga
    10081 atcgctccca taactcgaca aaaaagatca tgaaaaatgg gcgaggttgc tgcagcatcc
    10141 ccatgggtgt tgttgtattc atatttatta accttccaat accatcctga aaagtatgaa
    10201 cgcgtaaggg ataacgcgcg aaaaagttta catctgtcag agattcttaa tattgatgcg
    10261 gtgaatagat taatacacta ttacaacaga aaataaccag ctgatcacac taatgatgga
    10321 aaatatgatg gtgttaaata ttgtattggc tggggatttt gattgcgaac gcagaccgta
    10381 ggccagataa ggcgtttacg ctgatcaggt aacaaggcgc gacaaaaagc aaaacgcccg
    10441 catatgcggg cgttcaggaa cacgtcgtgg agggaagatt attcttcttc gtcgcgcagc
    10501 ggaacaatca gcatatcaac gtgaacggtg ttgatcagct gacgtgcgga agacatcagt
    10561 ttgctccaga agtcctggtg gtgaccacaa accactagat ccatatcgta tttcttgatt
    10621 gcatcgacca gaacctggcc caggtcgccg ctgccgctca gggtttcagt gattgggtag
    10681 cctgcattag tggaaagctc ggtcagcgca tgatgtgtct cttcagagat gcgtttctgc
    10741 atatcaccaa gattcacatc aataagcccg gtatataggt cagagtagtt tacatctacg
    10801 tggatcagag aaactttcgc attgtatggg cgagccatag agactgcttt ctctaccaga
    10861 actttgcttt ccggggagag atcgaccgcg atgagaatgt gtttataagc catagtgtta
    10921 ctccttccat aaagttgtcg atgactggcc agctagcgtt tcttgtgccg ttacgtcacc
    10981 cgcgtcctgc gaactacgtg cgcggggcta acccagccgt aaagattcag tgttaagacc
    11041 atccttacct tatagcgacc cggatgatcc gtcaatccgc cttgcttacc cgttagataa
    11101 acaaattttc gacatgaata tattgatagt ggttaacctt ctggaaaaaa aacaacctga
    11161 tctcctacac tatctataga gccgctcgga tgttggtcac cacaaaagcg gtttctccag
    11221 gacctggtgt taactgattg gcagcgtatc gggagcggta gccccggggt ggaactccgc
    11281 gggcaggtcg ccggggagga ggtatgataa gcaccgtcgc attattttgg gctttatgtg
    11341 tcgtttgcat tgttaacatg gcgcgctatt tctcatcact acgcgcgttg ttagtggtac
    11401 tgcgtaactg cgatccattg ctctatcaat atgttgatgg agggggcttt tttacctcac
    11461 atggccaacc caacaaacag gtgcgtctcg tttggtatat ctatgcccaa cgttatcgcg
    11521 atcatcacga tgatgagttt attcgccgct gtgagcgggt gcgtcggcag tttattctga
    11581 ccagcgcatt gtgtggtctg gtggtggtca gcctgattgc cttgatgatt tggcattaag
    11641 cacttcagca ataataacgc ataaaaaaag cgggccagta aactgacccg ctcgttttgg
    11701 tgcgtactat tacaggaact gcaaggagag ccagtaaagc ccgccggaaa gcagtaccgc
    11761 agccggaagg gtaaacaccc aggccatcag aatgctggtc acggttttac gctgtaagcc
    11821 gccaccatct accaccatcg tccccgcgac agaagaggag agtacgtgag tagtggaaac
    11881 cggcatcccg gtataactcg ccaggccgat agacactgcc gcagtcatct gggcagacat
    11941 cccctgagcg taggtcatgc ctttcttacc gattttctca ccgatagtcg ttgccacacg
    12001 acgccagcca atcatcgtac cgatacctaa cgccagcgcg accgccatga tgatccacac
    12061 cggtgcatac tcgatggtgc taagcatgtc ggacttcagt ttcttcaaca ggcgctgatc
    12121 gtcagcactc acgccaggca tcttcaccac tttgtcgata gtgtcagaaa cgcacagcat
    12181 aatgcggcgc atctggctac gttgatcaag cgacagcttg tcgtagcttt ccacatcggt
    12241 ggtcaacata cctttcaggc ggttgagcgc gttaatggta ttcgacggat ggcagtggaa
    12301 ctccgcaggt tgcgttgcgc cagcttccgg agccggtact aactgatcag caccagtagc
    12361 ttgtttcagc agcgcaggat gctgctcaaa gtaagcttcg acgttgttga tggcatcacg
    12421 ggtacgggtg atttcgtagc cagtggcatt catgttcacc acgaagcctg ctggtgcgac
    12481 accaatcaat accaacataa ccagaccaat gcctttctga ccatcgtttg cgccgtgcga
    12541 aaacgccacg ccgatagcgg aaaggatcag cgcgatacgc gtccagaacg gcggcttttt
    12601 cttgccgtct ttcttttcac gctccgctgg ggtcaggtgg atacgggcgc gtttcttggt
    12661 gccgctccag tagcgacgca gcaagaaaat cagaccgcca gcaaacacca ggccgacaat
    12721 aggggaaacg atcagagaac cgaaaatact taatactttc gggatattga gtgcatccac
    12781 cactgacgtc ccggtcatca acgcattggt taaaccaatc ccgatgatcg cgccaatcag
    12841 cgtatgagag ctggatgcag gcaaaccaaa gtaccaggta cccaggttcc agataatcgc
    12901 cgccagcaac atagagaaca ccatggcaag gccatgagac gatcccatat taagcagcag
    12961 atccgtcggc agcatatgca caatggcata ggcaacactc agaccaccca gcaaaacacc
    13021 caaaaagttg aacaccgctg ccataaccac ggcgagctga gaacgcatcg cgcgggtata
    13081 gataacggtt gccacggcgt tggctgtgtc atggaaacca ttgatggctt cgtagaacag
    13141 cacaaaagcc agtgcaagca ataataacag cccggtatgc aaatccaggc cagcaaacaa
    13201 atgtagcata ggacgttacg ccattttgag gacatgaacg cggcgcatta tcagtgactt
    13261 tcgcggcgcg ggcaaagtga aatatagact ttttttgatt tgcctcctgt atggatttca
    13321 ctcaaaaaat aattatctta tataattcag gcaaatactt ccttttagta atattgatgc
    13381 tggtgcgacc actgaggaat ctttacaatt cacgcccgtt ttttctaaga ggagcgcaac
    13441 gtggaaaggt ttgatgccat tattataggc gctggtgcgg cgggtatgtt ctgttctgcg
    13501 ctggcaggtc aggcaggacg ccgggttctg ctgatcgata atggtaaaaa accagggcgc
    13561 aaaatcctta tgtctggcgg tgggcgctgc aactttacca acctttatgt cgaaccgggc
    13621 gcttatctga gccagaatcc gcatttttgt aagtctgcac tcgcgcgttt tacccagtgg
    13681 gatttcattg atctggtcaa taaacacggc atcgcctggc acgagaaaac gttagggcaa
    13741 ctcttctgcg atgactccgc gcagcagatt gtcgacatgc tggtggatga gtgcgagaag
    13801 ggcaatgtga ccgtcagatt gcgtagcgaa gtgctgagtg tggcgaagga tgatacaggc
    13861 ttcacgcttg aactgaacgg catgactgtc ggttgtgaaa agctggtcat cgcgaccggc
    13921 gggctgtcaa tgccgggcct gggcgcgtcg ccgtttggtt ataagattgc cgaacaattt
    13981 ggcctcaacg tgctgccgac ccgcgcgggt ctggtgccat tcactctgca taaaccgttg
    14041 ctcgaagagt tacaggtgct ggcgggcgtg gcggtgcctt ccgtgattac cgctgaaaac
    14101 ggcaccgttt tccgtgagaa cttactcttc acccatcgcg gcttgtctgg accggcggtg
    14161 ttgcaaattt ccagctactg gcaaccgggg gaatttgtca gtatcaatct gctaccggat
    14221 gtggacctcg aaaccttcct gaatgagcag cgtaacgcac atccgaatca gagcctgaaa
    14281 aacacactgg cggttcatct accgaagcgg ttggttgaac gcttacagca actcgggcaa
    14341 atcccggatg tttcgctaaa acagctcaac gtgcgtgacc aacaggcact gattagcaca
    14401 ttgaccgact ggcgcgtaca acccaacggc actgaaggct atcgcactgc cgaagtgacg
    14461 ctcggcggcg tggacaccaa cgaactctct tcacggacga tggaagcgcg caaagtgcct
    14521 gggctgtact ttatcggcga agtgatggac gttaccggct ggctgggggg ctataacttc
    14581 cagtgggcgt ggtcgagtgc gtgggcttgt gcgcaggatt tgattgcagc gaagtcgtgc
    14641 tgagctattt ttcctaaaga tgaaaattat tagagtcgca ttaaaaatga gctaattttg
    14701 atagtggcta tcttgtgatt attttctaat gagcccgtga actgaacccc ccatgcttaa
    14761 tataaggtgg agggaaaggt gattgaaaac tcacccagtg ggctgatctt tataaaatat
    14821 gaacaggttg aaaaaaaaac agtaactttg ctgtttttta tacttaacca ctatgcatta
    14881 atgctgcgtt atttcattct acctaagaaa aaccagcgtt acgcaaatgg ttaacgctgg
    14941 ttttatccgg tacgttgcaa ttatttttta gcagaacccg cttctaatat tgaaacgatt
    15001 gagaacaacg taaagcatac cgctcccaaa ccgaccagaa cccgcgaagg aataaaatat
    15061 cccatatcgg tctgcgccat ttccgcaaga aacgatgcta ggaaaaggca aaacaggcag
    15121 gtgaagacgg gaatcatcgg tattctgttg gctaacgaac aggtacgtct ccagaccagt
    15181 gccagtagcc agacttttga gaatatgctg taacagatca ttcccaagca aatgaggata
    15241 ataccgggag ccagtcgggc gcttgcatca gaactgacta agacgtatat gccctgcagc
    15301 accgtgattg agccgagaaa aataacccaa taacaccaca gccagtgctc tttcgttgaa
    15361 aacgtattac gtgtttgatg gacgattgtg gcaacaaggc caattagaca ggcacagatt
    15421 gcggttaacc cgagcaatac gtgaccggcg acataatgcg gagttatgtc ggcactacgt
    15481 aacagcgtaa tagaccaaat gaaaccgagc agggtgagta acacgggaac ggcaatgagg
    15541 caattaccga ttaatgaaga ataagcctgt accggtgttc cgtcgctctt gctccccgct
    15601 gcgtttttag gaatgagcag aaagtgaccg gatgacgctg ccaccgtcga tacacaggcg
    15661 gcaatcatac caacgccgaa aataacatgg ccggcgacaa actcgtctgc catcacatca
    15721 ttacctgcta acagtgccca accccatatc atggtgataa ttgaccccgc atagccaata
    15781 atggggaaca atgtattgta aaacgtatta acgccgcgcg tgagctgcga aatgataata
    15841 aatgcggtag tgaataatgc caggcatatg gctgccagag aaatgagcac atgacccgcg
    15901 acgaaatact cgctttgccc tgaactttcc agcacaaagc cacctaacgc gatacaaatc
    15961 aagcccataa ttaaagggat taatttgaaa agccacccga tatatatgtt cactttaaac
    16021 tcctcaggta tccctgctaa tattgaaatg
//
LOCUS       DBFIFI010000093        15869 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.093, whole genome shotgun sequence.
ACCESSION   DBFIFI010000093 DBFIFI010000000
VERSION     DBFIFI010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15869
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.093"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..242
                     /gene="xisR"
                     /locus_tag="N7B39_002823"
     CDS             <1..242
                     /gene="xisR"
                     /locus_tag="N7B39_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HGU2422777.1"
                     /translation="YQITPSKWCTERVLIASTGLKPGTIERARRKSWMQGKEYRHYAV
                     EGDPGHYSECLYNIEEIMRWIENQKQPGAKNASSG"
     gene            276..1562
                     /locus_tag="N7B39_002824"
     CDS             276..1562
                     /locus_tag="N7B39_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU2422778.1"
                     /translation="MSNASYPTGVENHGGSLRIWFHYNGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRMGSFDYAAQFPNSPNLKHFGLGKREITVKALSEKWLDLKKIEICAN
                     ALNRYQSVIKNMLPMLGEKKLVSSITKEDLLFVRRDLLTGYQKLSNGKTSSIKGRSVV
                     TVNYYMTTIAGMFQFATDNGYTSGNPFNGLAPLKKSKVKPDPLTRDEFIRFIEACRHQ
                     QTKNLWILAVYTGIRHGELVSLAWEDIDLKARTITIRRNYTKLGEFTPPKTDAGTGRT
                     IHLVQPAIDALKSQAEMTMLGKQHSVEVKQREYGRTAVHKCTFVFSPQVTKQQQLSGP
                     HYKVDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMV
                     FNVYGAWMKDNNHEQIELLNKRLSESVPCMPHKKAG"
     gene            1583..2284
                     /gene="mlrA"
                     /locus_tag="N7B39_002825"
     CDS             1583..2284
                     /gene="mlrA"
                     /locus_tag="N7B39_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HGU2422779.1"
                     /translation="MSLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYP
                     AQTLTTHLFIPLRRRLQYQQPTLQALLAILDGVLINYIAICLASARKKQGKDALVVGW
                     NIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQL
                     TSWQEQGHDIFPLGI"
     gene            2344..2451
                     /gene="yohO"
                     /locus_tag="N7B39_002826"
     CDS             2344..2451
                     /gene="yohO"
                     /locus_tag="N7B39_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HGU2422780.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(2432..3163)
                     /gene="yehW"
                     /locus_tag="N7B39_002827"
     CDS             complement(2432..3163)
                     /gene="yehW"
                     /locus_tag="N7B39_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HGU2422781.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(3168..4094)
                     /gene="yehX"
                     /locus_tag="N7B39_002828"
     CDS             complement(3168..4094)
                     /gene="yehX"
                     /locus_tag="N7B39_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HGU2422782.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGELRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPSGTLHFQDLLVEA"
     gene            complement(4087..5244)
                     /gene="yehY"
                     /locus_tag="N7B39_002829"
     CDS             complement(4087..5244)
                     /gene="yehY"
                     /locus_tag="N7B39_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HGU2422783.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLASALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAGLVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLIDALFDLLIALLKVKRND"
     gene            complement(5251..6168)
                     /gene="osmF"
                     /locus_tag="N7B39_002830"
     CDS             complement(5251..6168)
                     /gene="osmF"
                     /locus_tag="N7B39_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HGU2422784.1"
                     /translation="MPLSKVWAGSLVLLAAVSLPLHAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLRRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYDFKLDQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAAQGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(6420..8717)
                     /gene="bglX"
                     /locus_tag="N7B39_002831"
     CDS             complement(6420..8717)
                     /gene="bglX"
                     /locus_tag="N7B39_002831"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HGU2422785.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITILQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            8913..10628
                     /gene="dld"
                     /locus_tag="N7B39_002832"
     CDS             8913..10628
                     /gene="dld"
                     /locus_tag="N7B39_002832"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HGU2422786.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGHG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(10666..11598)
                     /gene="pbpG"
                     /locus_tag="N7B39_002833"
     CDS             complement(10666..11598)
                     /gene="pbpG"
                     /locus_tag="N7B39_002833"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HGU2422787.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(11772..12359)
                     /gene="yohC"
                     /locus_tag="N7B39_002834"
     CDS             complement(11772..12359)
                     /gene="yohC"
                     /locus_tag="N7B39_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HGU2422788.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGDMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            12529..13107
                     /gene="yohD"
                     /locus_tag="N7B39_002835"
     CDS             12529..13107
                     /gene="yohD"
                     /locus_tag="N7B39_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HGU2422789.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGLAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(13237..13998)
                     /gene="yohF"
                     /locus_tag="N7B39_002836"
     CDS             complement(13237..13998)
                     /gene="yohF"
                     /locus_tag="N7B39_002836"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU2422790.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGNLPEGAQALEKLIQRLGRIDVLVNNAGAMTKVPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(14051..15487)
                     /gene="mdtQ"
                     /locus_tag="N7B39_002837"
     CDS             complement(14051..15487)
                     /gene="mdtQ"
                     /locus_tag="N7B39_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HGU2422791.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLIALTNHQTKSLTLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            15711..15794
                     /gene="yohP"
                     /locus_tag="N7B39_002838"
     CDS             15711..15794
                     /gene="yohP"
                     /locus_tag="N7B39_002838"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HGU2422792.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
BASE COUNT         3914 a         4295 c         3967 g         3693 t
ORIGIN      
        1 tctatcagat aacccccagc aaatggtgta cggagagagt ccttattgca tcaacagggc
       61 taaagcccgg caccatcgag cgggccagaa gaaagtcatg gatgcaggga aaagaatacc
      121 gccattacgc tgtagaaggt gatcctgggc attacagtga atgcctgtac aacatcgaag
      181 aaattatgcg atggatcgaa aaccagaaac aaccaggtgc caaaaatgca agttccggtt
      241 aacctgttaa tgctcctgga cgtctgggag gtttaatgag taacgcatca tacccgacag
      301 gcgttgaaaa ccatggcgga tcactccgta tatggtttca ctataatggc aaacgtgtca
      361 gagaaaacct cggtgttcct gacacagcca aaaaccggaa gatcgctggt gaacttcgca
      421 cttccgtttg ttttgcaatc agaatgggga gtttcgacta cgccgcgcag ttccctaatt
      481 cccctaacct gaaacacttt ggtctgggaa aaagagagat aaccgttaag gcactttcgg
      541 aaaaatggtt ggaccttaag aaaattgaga tttgtgcgaa tgcacttaac cgttaccagt
      601 cagtaattaa aaacatgtta ccaatgttag gtgaaaaaaa actggtttca tccataacaa
      661 aagaggattt acttttcgta aggagagatt tgttgaccgg ttaccaaaag ctttctaatg
      721 gaaagacttc ttccataaaa gggcgctcag tggtcacggt aaactactat atgacaacca
      781 tagctggaat gtttcaattt gcaacagata atggttatac ctcaggaaac ccatttaacg
      841 gtctggctcc cttaaaaaag tccaaggtaa aaccagatcc tctcacccgt gacgaattta
      901 ttcgttttat tgaggcttgc cgtcatcaac aaacaaaaaa cctgtggatt ctcgctgtat
      961 acacgggtat tcgtcacggg gagttggtat cgctggcatg ggaagatata gaccttaaag
     1021 caaggactat aaccatccgt agaaattata caaaacttgg cgaattcact ccaccaaaaa
     1081 ccgatgcagg caccggaagg acaattcatc tggttcaacc agctattgat gctcttaaaa
     1141 gccaggcgga aatgaccatg cttggaaagc aacattctgt agaggtgaag cagagggaat
     1201 atgggagaac tgctgtgcat aaatgcactt ttgtttttag tcctcaggta acaaaacagc
     1261 agcagttgtc cggacctcac tacaaggttg actccatcag ggagtcatgg acaagtatct
     1321 taaaacgcgc aggtctgaga cacagaaaat cgtaccaatc caggcatact tatgcatgct
     1381 ggtcacttgc cgcaggagct aatcctagtt ttatcgcaag ccagatgggc cacacaaacg
     1441 cacaaatggt attcaatgtt tacggagcat ggatgaaaga caacaatcac gaacagatag
     1501 aactccttaa caaaagacta tctgaaagtg tcccatgtat gccccataag aaagcagggt
     1561 aaaataaaaa cttgcaaaat caattagttt acccttaatc cctgtcacgt tacgcgcgtg
     1621 gcagaggcgt tacggattgc tgaaaccgca acggacagac ggcggtcatc ggctgttcaa
     1681 cgatgccgat attgaccgga tccgcgagat caaacgctgg atcgacaacg gcgtgcaggt
     1741 cagcaaagtt aaaatgctgc tcagtaatga aaatgttgat gtgcagaacg gctggcgcga
     1801 tcagcaagaa acattactga cctacctgca aagcggcaat ctgcatagcc tgcgaacgtg
     1861 gatcaaagag cgcggtcagg attaccccgc ccagacgctc accacacatc tgtttattcc
     1921 tctgcgccga cggcttcagt accaacaacc gactctccag gcgctgctgg cgatcctcga
     1981 cggcgtactg atcaactaca tcgccatttg tctggcttcg gcacgtaaaa aacagggtaa
     2041 agatgcgctg gtggttggct ggaatattca ggataccacc cgtctgtggc tggagggctg
     2101 gattgccagt caacaaggat ggcgcattga tgtcctcgcc cactcgctca atcaactacg
     2161 ccctgaacta ttcgaaggcc gtacattgct ggtgtggtgc ggtgaaaatc gaacctccgc
     2221 ccaacagcag caactcacca gttggcaaga acaaggccat gatattttcc cactcggcat
     2281 ttaatgattc gttaacaaat gcgctttact gtacaatcct ttcgttaaca taaggagtgc
     2341 attatgcgca tagctaaaat tggggtcatc gccctgttcc tgtttatggc gttaggcgga
     2401 attggtggcg tcatgctcgc aggttatacc tttattttgc gtgctggcta agcgcctgca
     2461 ccagcctttc aaacaggcgg tctgcgatga tcgccgccag tgccaccagt aacgccccct
     2521 ggatcacata cgcggtatta aatccgctaa gcccgatgat gatgggcgta cccagcgtgc
     2581 tggcccctac cgttgaggcg atcgtcgccg taccaatgtt gataatcacc gaggttcgca
     2641 cgcccgccag aatcaccgga gccgccagcg gtagctcgac cttacgcagt cgctgacctc
     2701 gactcattcc catacctttc gcaacttctg tcacgctggc atcaatcgct cccagcccgg
     2761 caagtgtcgc ctgcaggacg ggcagcacac cataaaggat caaggcgata atcgctggtt
     2821 gcagaccaaa gcctatcacc ggaacagcaa tcgccagcac tgcgacaggc ggaaaagtct
     2881 gcccaacggc ggcaatagtt tccaccagtg ggcgaaattc cgcgccccac gggcgagtga
     2941 ctgcaattcc ggcaccagtg ccaatgatca ccgcaaacaa actcgaaatt cccaccagcc
     3001 agaaatgagc cagtgccaaa gctgcaaaac tttcttgctg ataaacgggt cgtggcagtt
     3061 gtgggaacaa ggcagcaaac agcggctggc tgtaaggcag ccagaaaatc agtgccacaa
     3121 acaaagcaat gagccagaac agcggatcgc gcaacatctt catacgctta cgcctccacc
     3181 agcagatcct gaaaatgcag cgtgccgcta ggctggccct gcgtgttcac caccggcagc
     3241 acctcgcatc cccgcgcgac aaacagggag agcgcatcgc gcagcgtcat ctcttctgcc
     3301 agtgcctcac cttctgcccg ttcttcgcga cgcacgtaat ccgccacact acgtaacgaa
     3361 agcaggcgca cacccagttc actacgtcca aaaaactggc ggacaaaatc attcgccgga
     3421 cgagtcagca tcgtcagcgg attcccctgc tgcaccactt caccgtgatc catcaatacc
     3481 agatgttctg ccagccgtag cgcctcatca atatcatgcg tgaccagcac aatggtacgc
     3541 cccagcaaac ggtgaatgcg cgtcatctct tgttgcaacg cgccgcgcgt taccgggtcc
     3601 agtgcgccaa aaggttcatc catcagtaag acttgcggat cggcagccag tgcgcgcgct
     3661 actcccacac gttgctgctg accaccggaa agctgatgtg gataacgctc acgtaaattt
     3721 gactccagcc ccagtagcgc cattaattcg tcgatacgat cgtcaatcct cgcccgcgac
     3781 catttttgta attgcggcac ggtggcaatg ttttgtgcca cgctccagtg gggaaacagg
     3841 ccaatagatt gaatggcata gcccatacgg cggcgcaact ccagtactgg cagcgagcga
     3901 atttcttctc cagcaaagcg gagctctccg ctgtcatgtt ccaccaggcg gttaatcatt
     3961 ttcagggtgg tggatttgcc ggagccagat gtgccaatca gcaccgaaaa actcccttcc
     4021 tgaaaattga gattgagatc gttaacggct ttttgtgcgc cgaacagttt gctgacatgg
     4081 ctaaattcaa tcattacgtt tcaccttcaa cagtgcgatt agtaaatcga acagcgcatc
     4141 gatcagcacc gccagaacaa ttaccgggat cacccccagc aacactaaat caatggcgct
     4201 gcttagcagc ccctggaaaa ccagcgcacc aaaaccgcct gcgccgatta acgccgcaat
     4261 caccgccatg cctacagttt gcaccatcac cacccgcagg ctgcgcagaa ataccggtaa
     4321 cgccagcggt aactgaacat gcaggaatcg ctgcgccccg ctcatgccca tcgctctggc
     4381 gctctccagc acatcgcgcg ggatctggtt caagccgact accacgccgc gcaccagcgg
     4441 tagcaaggca tagagcacca gcgcaatcag tgcgggtgtc attccggttc ctgctatgcc
     4501 aagcttcccc agccacggaa aggccgtaac cagtccggca agcggcgcaa tcagcaggcc
     4561 aaagagcgcc acggaaggca cggtctgaat gacattgagc agagaaaaaa tcgccccctg
     4621 ccgcgcggtg gaaaagtagc accagatgcc caacggcaca ccaatcacta acgcaggcag
     4681 caccgcacca aacagcaacg tcagatgttg tgccagcgcg tcgtcaaaca catcctgacg
     4741 gttggcgtat tctttcatta gtgagagatc gttaagcgtg ccggagtaca gcaaccacag
     4801 cggaataatg gcaatctgca tatgcaacaa ccagcgccac agcggatgcg tggagattcg
     4861 gcggatggca tcgctacagg ccagcaatgc cagcgccgaa gccaaccaga aaccactgcc
     4921 gaggctggta cgcgccagcg cactgccatt ttgcgccagt tgggtcgccg cctttccagc
     4981 tccccacacc agcaatacga agacgaattg cgccagaatg agtgcaaaaa tgctgccttt
     5041 tttagcggga ataaaacagg ctgtcagcca ggcgcaacca acgcccacca gcatccagat
     5101 cgtttgcggc cacagttgcc agagatgacg cccttcaccc gagactaaac gattaggcgc
     5161 gtagctgata aacggcagcg ctgctgcgat tgccgtcagc aacagcagca gcgccagaac
     5221 aggattaata cgtagataag tcacgggaaa ttacttcgtc cacccttttt gtttcaagta
     5281 gtcggcagcc acttttttgg catccagtcc ttccactgca atgctggcat tcagttgctg
     5341 caatgttttt gcatcgaggc tggcgaagac tggctgtagc cactgtgcca tttgcggata
     5401 ctctttcagc accgactcac gcaccactgg tgcaggcgcg tagataggtt gcacgccttg
     5461 cggatcgctt aaggtttgca gcccctgcgc cgcgaccgga ccgtcagtgc cgtaagccat
     5521 tgcggcatta acgccagaag tttgctgggc agcggcttta atcgttaccg ccgtgtcgcc
     5581 gccagccagt gacagtaact gatcctgatc gagtttaaag tcgtaggctt tttcaaacgc
     5641 gggtaacgca tcggcgcgtt cgataaactc agccgaggct gccagtttga aggtgccgcc
     5701 ctctttcaga taacgcctca ggtcagcaag cgaagtgagt ttgtttttct ctgccacatc
     5761 ctgacgcacg gcgatggtcc aggtgttatt tgcaggtgcg ggcgtcagcc agattaactt
     5821 gttttgctct gcatcgagtt ttttgacttt ctcgtaacct tgctgcgcgt ttttccacgc
     5881 tgcatcgttt tcatctttaa agaaaaacgc gccattgccg gtatattccg gatagatatc
     5941 cagttcaccg gaagtaatcg ccccgcgcac cacaggagtc gttccaagtt gcactttatt
     6001 gaccgttggt actccgtggc tttcgagtac ttgcaaaatg atattgccga gcagcgcgcc
     6061 ttcggtatcg atttttgaac cgactttaac gggggaagcc gcgtgcagcg gcaggctcac
     6121 ggctgccaac aaaaccagtg aacctgccca gacctttgag agtggcatga tgctttcctc
     6181 attcttttac tgttgttttc agcgaattaa gagaaaagca tagttgataa tggcgggatt
     6241 agcctgagcg gacggattca gttgcagaat cagataaata cttaagaggc atattcggtc
     6301 cggcgtttcc tgccggatgc ggcgcgagcg ccttaaccgg cctacaaagg gcgaaaattc
     6361 agcaagttaa gatatgaaca tcaggcaaag ggcgcaaacg tcgcgccctt gctaaagcat
     6421 tacagcaact caaactcgcc tttcttaacg cgtgcggaat cagtgccgat aaagacattg
     6481 aacttgccag gctcggcgtc atatttcatc tgttgattcc agaacttcag cgcctcgata
     6541 tcaatcggga agctgacggt ctgggtttcg cccggcttca gggtgatttt ctcaaagcct
     6601 ttcagctgct tcactggacg gctcatggaa gccgtcacat cctgcaagta catctgcact
     6661 accgtggccc cttcgcgttt accggtgttc gtcacctgca cgctggccgt cactttgccg
     6721 tcacgcttca tggtcggcgc agaaagtttc acatcagaga cggtgaaagt ggtatagctc
     6781 agaccatagc cgaacggata aagcgccccg ttagcttcat caaaataacg cgaagtgtat
     6841 ttgttcggct tgtcggcatt atacggacga ccggtgttca gatggctgta gtacaccggg
     6901 atctgcccga cagaacgcgg gaaggacatc ggcagcttac cggacgggtt gtaatcgcca
     6961 aacaacacat cggcaattgc attaccgcct tcagtcccgg caaaccaggt ttccagaatc
     7021 gcatcagcct gctgatcttc tttcaccagc gccagcggac gcccgttcat cagcaccagc
     7081 accagcggtt tgccggtggc tttcagggcg gcaatcaaat cacgctggct ttgcagaata
     7141 gtaatatcgg tacggctgga ggcctcgtgc gccatcccct gtgcttcacc gactacagcc
     7201 accaccacat cagattgttt cgccgtctgc accgcttcat caatcatctc ttgcggcgaa
     7261 cgcggatcga ctttgaccgc ttcttcatac tgattcagga aatcgataat gcctttgtca
     7321 ctggtaacgt tcgccccttt ggcatacagc actttaccgt tttcaccgac ggcattttta
     7381 atcccggtca gcacggtcac ggattgatcg gcaacacctg ccgccgacca gctgcccatc
     7441 acgtcacgtt tactgtccgc cagtggccca accaccgcaa tggtggctga ttttttcagc
     7501 ggtaacgttt cgagacggtt tttcagcaac accaggcttt cacgcgccac ttcacgcgct
     7561 tctttgcggt gcaggcggct ttcggcattg gtatccaccg ggtcagactc tttcggaccg
     7621 agatggctgt atgggtcgtt aaacaacccc atatcatatt taacgttcag tacatggcgg
     7681 gcagcatcgt ccagctctgc catcgtcact ttaccggatt tgatcaaccc aggcagatac
     7741 ttcgagtagt actcgtcgct catgctcatg ttgattccgg atttcagcgc cacgcgcact
     7801 gcatcttccg gatctgccgc cgtgccatgt ttaatcagct ctttgattgc accgtgatcg
     7861 gaaacggtga tgcctttaaa gccccactgg tcgcgcagaa catctttcag cagccaggag
     7921 tcggaggtgg ctggcgtgcc gttcagcgag ttcagcgcca ccatcaccgc gccgctgcct
     7981 gcgtccagcc ccgctttgta cggcggcata taatcattaa acaggcgctg cggactcata
     8041 tcgacggtgt tgtactcttt accgccttct accgcgccgt atgcggcaaa gtgtttgacg
     8101 ctggtcatca ccgagtaacg atctgccggg cttttaccct gcatcgcttc caccatggtt
     8161 ttacccatta ttgaggtgag atacgtatct tcgccaaaac cttcggaagc acgtccccag
     8221 cgcggatcgc gcgagacatc gaccatcggt gcccaggtca tattcaggcc atcatctgcc
     8281 gcttcataag cagagacacg tccgaccgtt tttactgcat cgaggttaaa agacgaggcc
     8341 agaccgaggc taatcgggaa caccgtgcgc tgaccgtgca gcacgtcgta agcaaagaaa
     8401 agaggaattt tcaggcggct taattccatc acctgatcct gcatggcgcg gatatcctga
     8461 cgggttacgg tgttgaaaat agccccaacc tgaccgtctt tgatcatctc gcggatcgcc
     8521 tctttcgggt tatccgggcc gacgctgatt aagcgcagct gaccaatttt ctcatcaact
     8581 gtcattttct taagcagttc ggtgacgaac gcatcccgcg cttcaggcgt taatggatgg
     8641 ttaccgaata aatcatccgc cagtgctggc tgcagggcca gactcaccgc gattcctact
     8701 gaacatagcc atttcatatg gatttttctt cctttgttgc ccgacgtggc agcgaaaatg
     8761 gtgcaaaaac cgtagtttgc cataagcatg atggagagag aaaaagaatg ctcagtttat
     8821 tgtctgaatt ttcaaaatat tcactcgctg aattgtcata caaggcgcta ttctagtttg
     8881 tgatgttttt tcgccaccac aaggagtgga aaatgtcttc catgacaaca actgataata
     8941 aagccttttt gaatgaactt gcccgtctgg tcggtcattc acacctgctc accgatcccg
     9001 caaaaacggc ccgctatcgc aagggcttcc gttctggtca tggcgacgcg ctggctgtcg
     9061 ttttccctgg ctcactactg gaattgtggc gggtgctgaa agcctgcgtc accgccgaca
     9121 aaattattct gatgcaggct gccaatacag gcctgaccga aggatcgacg ccaaacggta
     9181 acgactatga tcgcgatatc gtgatcatca gcaccctacg tctcgacaag ctgcacgttc
     9241 tcggcaaggg cgaacaggtg ctggcctatc cgggcaccac gctttattcc ctggaaaaag
     9301 ccctcaaacc gctgggacgc gaaccgcact cggtgattgg ttcatcgtgc attggcgcat
     9361 cggttatcgg cggtatctgt aataactctg gtggttcgct ggtgcagcgt ggcccggcgt
     9421 ataccgaaat gtcgttattc gcgcgtataa atgaagatgg caaactgacg ctggtgaacc
     9481 atctggggat tgatctgggc gaaacgccgg agcagatcct tagcaagctg gatgacgatc
     9541 gcatcaaaga tgacgatgtg cgtcacgatg gtcgtcacgc ccacgattat gactatgtcc
     9601 accgcgttcg tgatattgaa gccgacacgc ccgcacgtta taacgccgat ccggatcggt
     9661 tatttgaatc ttctggttgc gccgggaagc tggccgtctt tgcagtacgt cttgatacct
     9721 tcgaagcgga aaaaaatcag caggtgtttt atatcggcac caaccagccg gaagtgctga
     9781 ccgaaatccg ccgtcatatt ctggctaact tcgaaaatct gccggttgcc ggggaatata
     9841 tgcaccggga tatctacgat attgcggaaa aatacggcaa agacaccttc ctgatgattg
     9901 ataagttagg caccgacaag atgccgttct tctttaatct caagggacgc accgatgcga
     9961 tgctggagaa agtgaaattc ttccgtccgc attttactga ccgtgcgatg caaaaattcg
    10021 gtcacctgtt ccccagccat ttaccgccgc gcatgaaaaa ctggcgcgat aaatacgagc
    10081 atcatctgct gttaaaaatg gcgggcgatg gcgtgggcga agccaaatcg tggctggtgg
    10141 attatttcaa acaggccgaa ggcgatttct ttgtctgtac gccggaggaa ggcagcaaag
    10201 cgtttttaca ccgtttcgcc gctgcgggcg cagcaattcg ttatcaggcg gtgcattccg
    10261 atgaagtcga agacattctg gcgctggata tcgctctgcg gcgtaacgac accgagtggt
    10321 atgagcattt accgccggag atcgacagcc agctggtgca caagctctat tacggccatt
    10381 ttatgtgcta tgtcttccat caggattaca tcgttaaaaa aggcgtggat gtgcatgcgt
    10441 tgaaagaaca gatgctggag ctgctacagc agcgcggcgc gcaataccct gccgaacata
    10501 acgtcggtca tttgtataaa gcaccggaga cattgcagaa gttttatcgc gagaacgatc
    10561 cgaccaacag tatgaatccg gggatcggta aaaccagtaa gcggaaaaac tggcaggaag
    10621 tggagtaaaa attacggatg gcagagtatc gccatccgaa ttcacttaat cgttctgtgc
    10681 cgtctgcccc gccgccgcca tttgggcggc tttttgtttt ttatagctta acgctgctgc
    10741 cggaacaggc atcactttac cggtttcaat ccaggtacgc aggcggctgg catcggcaaa
    10801 atgggtgtat ttgccaaacg catccatcac taccagcgcc accggtttat tattgataac
    10861 cgtacgcatc accagacaat ggcccgccgc attggtaaag ccggttttgg ttagctggat
    10921 attccagtta tcgcgataca ccagatgatt agtattgcgg aacggcagcg tatacgtcgg
    10981 gttagagaag gttgccatat cttcccgggt agtacttaac tgcccgatca acggatattg
    11041 tttgctggca atgagcagtt tggttaagtc acgggcggtt gaaacgttat gcaccgacaa
    11101 tccggtaggt tcaacaaagc gcgtgttgtt cattccgagc gatttcgctt tcgcattcat
    11161 tgccttaata aaggctttgt aaccaccggg ataatggtgc gcaaggcttg ccgccgcgcg
    11221 gttttctgaa gacatcagcg ccagcaacag catatcttta cggctgattt cgctattcag
    11281 tcgtacgcgc gaatagaccc ctttcatctc cggcgtctgg ctgatatcca cttttagttt
    11341 ttcatccagc ggcagtcgtg catccagcac gaccatcgca gtcattaatt tgctgataga
    11401 cgcaatcgga cgcaccagat ccgggtggtt cgaatagatc actttgttgg tattcagatc
    11461 aacaatcatc gcgctaccgg aggcaatttc cggttgtgaa gcggtggtag cggctgccgt
    11521 tttcgcaacg gcctgcggtg ccaaaggcac agccagcatc agggccaggc taaataaaga
    11581 aactcgaaat ttcggcatga tgagcattca gatagtggtt cacgcgcacg ggttgcgcac
    11641 cgccggagta aggatttact gaggctagcg acgccatcat aacgagcaaa aagtgcgatc
    11701 gtcaaaggag aatcgtgagg aaatgctgca ttgctgacat ttacgccagc aatgcaacgt
    11761 caaagaactt tctagaacaa acgataaccg tagccccata gtataacggt aagggcgagc
    11821 agcacttcca gtaccagcac gccaatcgcc agcgtcgaac tggagaagct aaggccttcc
    11881 tctttgttga tattcaggaa gctcggaata ccaaggtaaa gtaggtaacc ggtgtaaaac
    11941 agtgccaccg tgccgaccag cgcacacaac cagaccagcg gataaagcgc caccagacca
    12001 cttaaaaaca gcggagttgc aacgtagccc gcgaagacca tgcagtgcgc aagtgacgga
    12061 cgctgcggat aattacgcgc catccaccag atgacccgcc ccatcaccgc gaccccagcg
    12121 agcatatcgc cataaaacag gacagccagc gccagtccgg taaaccagga taacttcagg
    12181 atagtgccat cgccaaaatt ccagccaatc tgtgtagtgc caatgaaggc gcaaatcacc
    12241 ggaatcgccg ccatcagcaa aacgtggtgg gtgtaatggt gagaaatcgt ttcgttttcg
    12301 cgattaatca cctgcatttc acgatcggga tgggaaaaca gtccccagac atggctcata
    12361 ccgccccctt gttgtgagtt catgaacctg acagttcaag tataagtcag cttgtgatta
    12421 ttttttgttc gcccatgtaa tttgcaccgt ctgatgtacg tcctttttca gcgtgaatga
    12481 ttcacagggt gtatgcttac aggcaaccaa agggagacag actggcctat ggatctcaat
    12541 acactcatct cacaatatgg ttatgccgcg ctggttatcg gtagcctggc ggaaggtgaa
    12601 accgtgactt tgctgggagg ccttgcggca catcaggggc tattaaagtt cccgctggtg
    12661 gtactttctg tagcgcttgg cggcatgatt ggcgaccagg tgctctatct gtgcgggcgg
    12721 cggtttggcg gtaagctgtt acgccgtttc tctaaacatc aggataaaat tgagcgggcg
    12781 cagaaactga tccaacgcca tccgtatctg tttgtcattg gtacgcgctt tatgtatggc
    12841 tttcgggtga ttggcccgac gctgattggt gccagccagc tgccgccgaa aatctttctg
    12901 ccgcttaata ttctcggcgc atttgcctgg gcgttgattt ttaccactat cggttacgct
    12961 ggtggtcagg tgattgcgcc gtggttgcac aatctcgacc agcatttgaa gcactgggtc
    13021 tggttgattc tggtcgtggt tctggtggta ggcgtgcgct ggtggctgaa acgacgcggg
    13081 aagaaaaagc cggataatca ggcgtaaaac cattgccctg gataaggcgt gcgcgccgca
    13141 tccgacatcc ggttcaccat gcctgatgcg gcgcttgtgc gtcttatcat gcctaccaaa
    13201 attttaccca ttgaaatatc cacacacctc actgcgctat tctgggttga actgcggatt
    13261 cgccaacata aagccgccat ccactatcaa cgactgcccg gtggtgtaat ttgcgccctc
    13321 cgaacaaagc cacgccacca ggctggcaat ctcatgcgtt gcgccaaaac gccgcagagg
    13381 aatcgaaggc tccgcgtcgg gcttcacgtc gctatcatcc atgccgttca ttggcgtagc
    13441 gatcgcccca ggtgcgactg cgttcaccaa aatcttatgc ctgaccaact ccagcgccat
    13501 tgctttggtt aacccgccga gcgcatgttt agcggctgtg taggcgctgg catccggcag
    13561 cggcgtatgt tcatgtaccg acgtaatgtt gatgatgcga ccaccctgtc cttgtttcac
    13621 catctggcgg gcagcaattt gcgagcataa aaatgcacca tcgacatcaa cggtaaaaat
    13681 cttgcgccac tcatcgaaag ccatatcaag aaacggcact ttggtcattg cgcccgcatt
    13741 attcaccagc acatcaatac gccccagccg ttgaataagt ttctccagcg cctgcgcacc
    13801 ttctggcaga ttgccgagat ccagttgcac gatctccgca cgtacgccgt ggctaactac
    13861 ctcacgcgcg gtatcttttg ccccttcttc atctgagtgc caggtaatgc caatatcaaa
    13921 cccttgctgc gccagtaata acgcgcactc tttgccgatc cccgaatcgg aggcggtaat
    13981 aatcgctacc tgtgccatcg agttctccac ttaacgctga ataaacgtta agtatagaag
    14041 tcacatatca tcagcgtttg taacccccgc ccaacgcacc agtgagttga atggaggcat
    14101 ccagccactg cccttgcaat aacaggccat tggcccgctc acgcagcgcg gggattctgg
    14161 cttcgctgac gcgggaacca gcaatgatgc ccgcgttaaa gcgggcctgc gcaagaccta
    14221 ccacacgcaa ggcatcgcgc tcaatttgcg cctgatgctg gtttttctcc gccagtgtct
    14281 gaacctgact ggctgcccgc gccacgtcat tcaccgcttc aaccaccgct ttgttgtagc
    14341 tggcgataga caagttgctt tcggcttttg cgatatcgag attggcgtta agacgaccac
    14401 tatcgaaaat gggtagcgtc aggcctgccg taacgcccat ttgctgcgcg gaatgacgga
    14461 acagatcgct taagtgcaac gcatcctgtt gcaggaaggc catcaggttg atgtcaggat
    14521 aaaatgccgc ttttgccgca tcaatggtgc ttagcgatga ctcaacgtac cagtgcgccg
    14581 cctgcaaatc tgcccgccgg gccagtaagg agtaccccag ttcatcagga agctggcttg
    14641 ccactttcgg caacgccacc ggtttaagcg tcaatgactt agtctggtga tttgtgagtg
    14701 caattaaccg tgcctcaata attttcattt tccccgcgac atcgttgagc tgctgccggg
    14761 ttttgctggc attaatatcg gtttccacac cttcaactga agaagtaatc ccgttctgat
    14821 atagctggcg atcggtcgcg ataatggtgt tctgctcttt ttcgatttgc tgcaagaccg
    14881 tgtttaacgc cgcctgggtt tgccactccc agtacaggcg ggctacgctg ccagccagca
    14941 attggcgggt ttgctcgcgt tccgccgccc gtgctttaac cgtacccagg cgggcagtaa
    15001 cctccgcccg attctttccc cagatatcga gatgccagcc cgccgttaag ccaaaagttc
    15061 cgttggtgta ccacggaccg gtcgtacctg cggccggatc gttcagagca aacggcccca
    15121 ttagcccttc tgccgacatt ttttgccgct ccatatccgc agaaaagtcg atctgcggac
    15181 catcctgagt agcaactgcc ttcgcctggg cttcagctaa ctgaatgcgc tgttcagcca
    15241 cctgcatatc cggtgcgttc tgtagtgcat tgttaattaa ggaagtgagt tgattatcgt
    15301 gatactccag ccaccattgg ctgtctggcc aaccattttt cagcgccgtg ggtaatgcgg
    15361 tgtcaacttg tgcagcgggc gtttgctggc ttaacgcctg gcgggtttca tgcataggcg
    15421 cacacccggc cagcatcagt aacagcggaa aacaggcgat ggctggataa aaggaatcac
    15481 gattcatggg ggaataatca ggtaagaaaa ggtgcgcgga gattaccgtg tgttgcgata
    15541 tattttttag tttcgcgtgg caatacatca gtagcaataa aacgacatat ccagaaaaat
    15601 atacactaag tgaatgatat cttccgattt atcttaatcg tttatggata acggcaaagg
    15661 gcttcgtttt ttcctatact tattcagcac tcacaaataa aggaacgcca atgaaaatta
    15721 tactctgggc tgtattgatt attttcctga ttgggctact ggtggtgact ggcgtattta
    15781 agatgatatt ttaaaaatca gatatgacat ataagttgtc aggtcaggaa ctagatattt
    15841 tgtgtacttg atttgcaaac agctccggt
//
LOCUS       DBFIFI010000094        15763 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.094, whole genome shotgun sequence.
ACCESSION   DBFIFI010000094 DBFIFI010000000
VERSION     DBFIFI010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15763
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.094"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(5..1054)
                     /locus_tag="N7B39_002839"
     CDS             complement(5..1054)
                     /locus_tag="N7B39_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HGU2422793.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            1186..2403
                     /locus_tag="N7B39_002840"
     CDS             1186..2403
                     /locus_tag="N7B39_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2422794.1"
                     /translation="MPEPVAEPALNGLRLNLRILSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDVKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(2407..2964)
                     /gene="dcrB"
                     /locus_tag="N7B39_002841"
     CDS             complement(2407..2964)
                     /gene="dcrB"
                     /locus_tag="N7B39_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HGU2422795.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(3037..3702)
                     /locus_tag="N7B39_002842"
     CDS             complement(3037..3702)
                     /locus_tag="N7B39_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HGU2422796.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            3923..4168
                     /gene="tusA"
                     /locus_tag="N7B39_002843"
     CDS             3923..4168
                     /gene="tusA"
                     /locus_tag="N7B39_002843"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HGU2422797.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(4270..6468)
                     /gene="zntA"
                     /locus_tag="N7B39_002844"
     CDS             complement(4270..6468)
                     /gene="zntA"
                     /locus_tag="N7B39_002844"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HGU2422798.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFATSWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAALAIPAAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(6542..7168)
                     /locus_tag="N7B39_002845"
     CDS             complement(6542..7168)
                     /locus_tag="N7B39_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HGU2422799.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            7309..7668
                     /locus_tag="N7B39_002846"
     CDS             7309..7668
                     /locus_tag="N7B39_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HGU2422800.1"
                     /translation="MSKPPLFFIVIIGLIIVAASFRFMQQRREKADNDMAPLQQKLVL
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(7671..7940)
                     /locus_tag="N7B39_002847"
     CDS             complement(7671..7940)
                     /locus_tag="N7B39_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HGU2422801.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(7930..8526)
                     /gene="rsmD"
                     /locus_tag="N7B39_002848"
     CDS             complement(7930..8526)
                     /gene="rsmD"
                     /locus_tag="N7B39_002848"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HGU2422802.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADETLIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            8676..10172
                     /gene="ftsY"
                     /locus_tag="N7B39_002849"
     CDS             8676..10172
                     /gene="ftsY"
                     /locus_tag="N7B39_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HGU2422803.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQTVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAENEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            10175..10843
                     /gene="ftsE"
                     /locus_tag="N7B39_002850"
     CDS             10175..10843
                     /gene="ftsE"
                     /locus_tag="N7B39_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HGU2422804.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            10836..11894
                     /gene="ftsX"
                     /locus_tag="N7B39_002851"
     CDS             10836..11894
                     /gene="ftsX"
                     /locus_tag="N7B39_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HGU2422805.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            12139..12993
                     /gene="rpoH"
                     /locus_tag="N7B39_002852"
     CDS             12139..12993
                     /gene="rpoH"
                     /locus_tag="N7B39_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HGU2422806.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            13264..14367
                     /gene="livJ"
                     /locus_tag="N7B39_002853"
     CDS             13264..14367
                     /gene="livJ"
                     /locus_tag="N7B39_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HGU2422807.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            14517..14783
                     /gene="ataR"
                     /locus_tag="N7B39_002854"
     CDS             14517..14783
                     /gene="ataR"
                     /locus_tag="N7B39_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin AtaR"
                     /protein_id="HGU2422808.1"
                     /translation="MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRA
                     AEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKRAAKRLQGM"
     gene            14790..15317
                     /gene="ataT"
                     /locus_tag="N7B39_002855"
     CDS             14790..15317
                     /gene="ataT"
                     /locus_tag="N7B39_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin
                     acetyltransferase AtaT"
                     /protein_id="HGU2422809.1"
                     /translation="MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKIL
                     RAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQKKIPYKNIPSVTLGRLAIDRSL
                     QGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENENAL
                     FFPTKSIELLFTQSD"
     gene            complement(15314..15697)
                     /gene="panM"
                     /locus_tag="N7B39_002856"
     CDS             complement(15314..15697)
                     /gene="panM"
                     /locus_tag="N7B39_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HGU2422810.1"
                     /translation="MKLTIIRLEKFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT         3849 a         4058 c         4284 g         3572 t
ORIGIN      
        1 gctattaacc cggtaaccga cttttcggtc tgcccggccc cagtaaaatc gccagtttgc
       61 tgccgccttt cgtcgtttcc atccagattt tacacacgct ggttaatggc accgaaagta
      121 gcatccctac cgggccgagc agccatcccc aaattaacaa tgaaagaaat accaccatgg
      181 tggacatccc caggcgatgg cccatcatcc gtggttctaa aatattgccg atgaccatat
      241 ggaccactaa aaacaatgcg ccgaccagaa tacattcgta aacaccatta aacagcagca
      301 cctgaatcat tggcggtacg gcggaaatta ccgcgccgat attgggcacg tagttaagca
      361 aaaacgccag tactgcccac atcagcgcaa actgtacccc catcagctcc agccccagcc
      421 agacgatgac acccgtccat aaactgagta gcgtctttaa cgccagataa tgcgaaacgc
      481 ctttaagtgc gcggtgtaat cccgcgatgt gaatctgtgg gttattcagc gcaaaacgca
      541 ttttgtaagg gacgtggcgc acttcaaaca gcataaaaac tacggtcatc accagcaaaa
      601 gcacgctcgc cattgccccg gaaagcccgg tcattagcgc tgtggtgaag gtaaccactt
      661 tttccgagtc catccgctgc agcattcgct ccggcgacat atgcaaatta agaaaaggca
      721 acatctcctg caatttaaaa agtttgcgcg tcagctcctt attaaacttc ggcagcatag
      781 agataaattc gttaaacgat gccgccagta cgccgaccag cgcggttagt gcgatcagca
      841 tcaccaccac tacaatcgta atggcaacgg ggcgttgtac tccccgacga ataaaccagg
      901 tgacgagcgg gttgaggacg atggcaaaaa acagcgccag taacagctgc acaatgatat
      961 ctgccgctgc gtgaatgccc gcgaggatca ctaccagcga ggccagcttg agcagaatgt
     1021 gcatgcccgt tttatcgggt tgaggggttt ccattggggc ttccttgtga ctttttgtat
     1081 taagtgtagc gggagtcacg acggcaatat tctgattctt actgctgatt ttcacgtcag
     1141 cacgtggtaa aaatgaaaca ctgttgtaaa aatgtggtga tcctcatgcc cgaacccgta
     1201 gccgaacccg cgctaaacgg attgcgcctg aatttgcgca ttctctccat tgtcatgttt
     1261 aacttcgcca gctacctcac catcgggttg ccgctcgctg tattaccggg ctatgtccat
     1321 gatgtgatgg gctttagcgc tttctgggca ggattggtta tcagcctgca atatttcgcc
     1381 accttgctga gccgtcctca tgccggacgt tacgccgatt tgctgggccc caaaaagatt
     1441 gtcgtcttcg gtttatgcgg ctgctttttg agcggtctgg gatatctgac ggcaggatta
     1501 accgccagtc tgcccgtcat cagcctgtta ttactttgcc tgggacgcgt catccttggg
     1561 attgggcaaa gttttgccgg aacgggatcg accctgtggg gtgttggcgt ggttggctcg
     1621 ctgcatatcg ggcgggtgat ttcgtggaac ggcattgtca cttacggggc gatggcgatg
     1681 ggtgcgccgt taggcgtcgt gttttatcac tggggcggct tgcaggcgtt agcgttaatt
     1741 attatgggcg tggcgctggt ggccattttg ttggcgatcc cgcgtccgac ggtaaaagcc
     1801 agtaaaggca aaccgctgcc gtttcgcgcg gtgcttgggc gcgtctggct gtacggtatg
     1861 gcgctggcac tggcttccgc cggatttggc gtcatcgcca cctttatcac gctgttttat
     1921 gacgttaaag gttgggacgg tgcggctttc gcgctgacgc tgtttagctg tgcgtttgtc
     1981 ggtacgcgtt tgttattccc taacggcatt aaccgtatcg gcggcttaaa cgtagcgatg
     2041 atttgcttta gcgttgagat aatcggcctg ctactggttg gcgtggcgac tatgccgtgg
     2101 atggcgaaaa tcggcgtctt actggcgggg gccgggtttt cgctggtgtt cccggcattg
     2161 ggtgtagtgg cggtaaaagc ggttccgcag caaaatcagg gggcggcgct ggcaacctac
     2221 accgtattta tggatttatc gcttggcgtg accggaccac tggctgggct ggtgatgagt
     2281 tgggcgggcg taccggtgat ttatctggcg gcggcgggac tggtcgcaat cgcgttatta
     2341 ctgacgtggc gattaaaaaa acggcctccg gaacacgtcc ctgaggccgc ctcatcatct
     2401 taaaacttac tgaataacca gcgtattaat gatgttttct gcggtggtct gcgctttttg
     2461 ctgatcgtca gctggcagag taatttgcat ggtcagcagt tgattaccca cgttacccag
     2521 aataacggaa gagtacgccg tctggccttt cgcggagata atactgtcta actgctgcat
     2581 tttgtgacct ttcagctcaa tggctttatt ggtgaccact tgcagctgtg gatcgcggct
     2641 acgttgctga tcttccagac gcttcgccag caccgccaga tcttctttcg gatcatcgcc
     2701 catgatgacg atgactgctt tctgcccggt ggcgtcggac cagacatgca tgttattggc
     2761 ctgcgttccc agcttaccgc tctggtcggt catatccgct ggcagcgaga aacttaactt
     2821 gccatcaagc aggttgacgg gattcccggt agcgttactt tccgcgaccg aaccctgcgc
     2881 cgtagcgtta gtgtctttat catcacaggc cgcaagcccc ataaccagca ggccaattcc
     2941 gacatattta accagattgc gcattgactt cttcctttcg ataaacggcc ataacggctc
     3001 attcatccat cttatcacaa ctctgataac gaacctttaa ctcgcctgca aagcgttgat
     3061 ttcggattta tctgccagtc ttttcaacag catattgagt aatacgccat acattggcag
     3121 gaagaaaacg atactgatta acactttgaa acagtaatcg accagcgcga tttccatcca
     3181 gtgttcagcc ataaaggcat ccgggctacg ccagaaggca atgaagaaaa aggccagcgt
     3241 gtcgctgacg ttaccgaaca gtgtggacgc tgtcggtgcc agccaccagc ggcgactctg
     3301 acgcaggcgg ttaaaaacgt gcacgtcgag gatctgtccc agcgcgtagg ccatgaaact
     3361 ggcggtggcg atacgggcga caaacaggtt gaagtgggcg agtgcgccga atccctgcca
     3421 tgaacccata tagaatagcg acgagatgac gtaggagatc agcaatgccg ggatcatcac
     3481 cgcaaagatg atacgacggg ccagcggtgc gccaaaaata cgtacggtca ggtcggtagc
     3541 gagaaagata aacggaaagc taaacgcgcc ccaggtggta tggaaaccaa aaatggagac
     3601 gggaagctga accagatagt tactggaggt gatcaccagc agatgaaata acgataacca
     3661 gaacaacgcc ttatagcgtt gagtttgcga gaaaacgttc atattgtacc tttttgatta
     3721 accattgggg tgagggaacc caatacgtac gacacgtctg ttaccttaac ataataacgt
     3781 gcttaccctt ttttcgagcc gccgcatgat actgctttac gttgacaatg caatggttaa
     3841 ttttcacgca atcgttaacc tggtttgctt acggactcac agggcgtaaa ctagcgccgt
     3901 ttttttatgt gatgagaaga aaatgaccga tctcttttcc agccctgacc acacactcga
     3961 cgcacttggc ctgcgctgcc cggaaccagt catgatggtg cgcaaaacag tgcgcaatat
     4021 gcagccaggt gaaacattgc tgattattgc tgacgatccg gcgacgaccc gcgatatccc
     4081 tgggttttgt acctttatgg aacacgaact ggttgccaaa gagacggatg gactgcctta
     4141 tcgttatttg attcgtaaag gcggttgacg ggggcagatt ggcttcgatg ccgccttttc
     4201 ccctcaccct aaccctctcc ccagaggagc gaggggaccg atcgcgctca atgttgcgat
     4261 cggtttgcct tatctcctgc gcaacaatct taacgcattc gctgtcacca gcaccgtcgc
     4321 acccgtatct gccagcactg ccagccatag tccggtcatc cctaacagcg tggtgacgag
     4381 gaagatccct ttcagcccca gcgcaatagt gatgttctgg cggatattgg cgtgagtggc
     4441 gcgtgccagt tcaatcattt gcaccagacc gcgcaggtgg ttatgggtta atgctgcgtc
     4501 ggcggtttcc agcgccacgt ctgtgccgct gcccattgca atcccgatgg cggcagcttt
     4561 catcgctggc gcgtcgttaa taccgtcacc gaccatcgcc agcggcgcat gttgattcag
     4621 ctcggtcacc gctttgactt tatcttccgg caacaggccc gctttaaact ccagccccag
     4681 ctccccggca attgccgccg ctgcgcgtgg attatcgccg gtgaggatca cccctttgac
     4741 gcccagcgcg ttcagttcac tgatggcagt tgcagcatcg gcgcgcaggg tatcctgcaa
     4801 tgcaatgata cccagcacgt catcattacg tactaccagc acaaccgttt gcccggcgct
     4861 ttccagttca ttaatcaaac cagcaaatgc atcagcggga tgtttcccgg cagcgcatat
     4921 caacacgcgc tcaccgttaa cctgcgcttc aatgccagac ccgaccagcg cccgctgtga
     4981 ttcggcggcg ggaatggcga gtgcagcaac ctgtgcttcg cgcacgatgg cctgcgccag
     5041 tggatgcgtc gcgccttgct cgaccgccgc cgccagtgtc agcagttcag attcactaat
     5101 acccgttgcc ggatgaatcg cggtaacgcg cggtttaccg acggtcagcg taccggtttt
     5161 atcaaacgcc acctgggtaa cacgacccag ctgttccagc gccgctccgc ctttaatcaa
     5221 cgccccacga cgcgctgccg ccgccagccc ggaggtaatc gccgcaggcg ttgagataac
     5281 taacgcacac gggcagccaa tcagcagcag cgtcagccct ttataaatcc actcctgcca
     5341 gctggtggca aacagcagcg gtggcaccag cgtcaccagc agagcgacgg ccataatcgc
     5401 gggcgtgtaa atacggctga aacggtcgat aaaccgctca atgggtgcgc gacgctcttc
     5461 ggcttcttcg atcagtttca gaatccggtc aatggcgctg gcacccggtt ctgacagcac
     5521 ttccagcgtt accagacggt ctacgctggt ggctcctgca ggaactttat cgcccgttgc
     5581 gcgctccacc ggaatagatt cgccggtcag ggcgctttca tcaaaactgg caaacggtga
     5641 gagcagttta ccgtcggcag gcaaacgtcc acctgcggcg acttcaatca catcgccagg
     5701 gcgcaggctg ttaatcgcca cctcttcccg ctcaccgtta cgcaggcgcg tggcggtttc
     5761 tggtttcagc gccattaacg cgctgacccc ttgacgcgcg cggctggcgg cccagccttc
     5821 cagtcgttca ccaatcaaaa acagcagcaa caccatcgca gcttcagccg ttgcgccaat
     5881 aaacagtgca ccaatagcgg ctacgctcat taaggtttca atggcgaagt agctgccgga
     5941 tttgatcagc cgtaatgcct gacgagcaat cgggtacagc ccaaccagcg tggtcgcgat
     6001 aaacgccagt tgcccgaacg gatgattaaa ttgctccaaa ccccagctga ttgccatcat
     6061 cacgataagc gtaatcagcg gcagattctc tttcaggcgc gatgcttgtg gctcgtcggc
     6121 ggcctgctca tcgcgcaggg aatagcccgc tttttgcacc gcagattcaa cttgtgcacg
     6181 gatgtcattg tcggcatcaa ccaccagttt ttcggtggcg aacaacacct gtacctgatt
     6241 cacgcctgca agctggcgca cggcattttc taccttgcgc gcacaggcgg cgcagtccat
     6301 gccgctgact ttccagctat agcgggtgcc ggagacgttt tcagagagag ttggcgtgct
     6361 ggaacatgcg ccgtcgcagc aacagtcgtt ggcgttctgt accgtggtta gcggtttgaa
     6421 cgcagcaaat tgaggggctt tcttgccgtg attgtcagga gtcgacatgg catcctccgg
     6481 ttaagttttt tctcattaac cgaaggatac actctggagt cgactccaga gtcaagtttt
     6541 atcagagata cagcgagcgg acgatgagga agtgaccggc aaagtagcag gccgcagcaa
     6601 tcgcgttgtc cgcacggaag cgtcggcgat agtgactacc cagccagaca aagttactga
     6661 taaacagcaa cgaagcgccg acaaacgcag agagtgccgg agcggtcgga cggaagaacc
     6721 acagttcacc tgccagccac accatcacca gcgtcatgcc gataaacgtg cagataggcc
     6781 aacgatactc ttccagccgc gtccagataa tcgccagtaa cagcgcaccc agaaccagca
     6841 acaccagtgg cagaggccag aagaaagaga gcgtcatctg actggcgaaa tagatggtgt
     6901 acagcaggtg cgagaggaaa aacgcgccga tggcgtacat cagacgttga cgtggcaaca
     6961 gggttagcgc atcgcccagc agtgaggcgc acagccctgc cagcaccaga tagctaatgg
     7021 cgtcgaacat cggcgcttgc caggccagta acagcaggag aagaagggtt aacggtttaa
     7081 acacccagcg ttgccaggtt ggcccacgat acgatgcatc cacagatagc catgcggaaa
     7141 gacagacagc gataaacgac caaagcatct tagttccttg aattcgttat tttttcgtaa
     7201 gcggaatgcc tacggtcttg cttccagttt agtggcgagg gtggacggat gacaacacca
     7261 taactggcag ggtatgctta tttccgcatt ttccgatgag ggataaagat gagcaaacca
     7321 cctcttttct ttattgttat cattggctta atcatcgtcg ccgcatcgtt tcgttttatg
     7381 caacagcgac gggaaaaagc tgataatgat atggcaccac tccagcaaaa gctggtgttg
     7441 gtgagcaaca agcgggaaaa accgattaac gatcgccgtt cgcgccagca ggaagtgact
     7501 ccggcaggca ccagtatgcg ctatgaggca agcttcaaac cgcaaagcgg aggaatggag
     7561 cagacgtttc gcctcgacgc ccagcagtac cacgccctga cagtgggcga taaaggtacg
     7621 ctgagctata aaggaacgcg ctttgttagc tttgttggcg aacaataagg ttatttttta
     7681 actttaaatt tcttctgcca gaccagcagt tcaaacacgc cgaaaaggaa aatccgcacc
     7741 ttttcggcgg tggtcatctg cgggccatct ttcggtaaag tggatttcaa tagcgccagt
     7801 tgcataccat gcatcagcac ggtaaaaatc agcgccacgt taacgaaaat attcagcggg
     7861 cgcgggaagg gatgcaccag gttgaggatt aaaaatcccc aaacacagag cattaacaaa
     7921 cgaccaatat taatcagcat cactttctcc ttgtgcttcg cgttgataca gccgataagc
     7981 cacctgaccc gccacttttt cccgatgtaa cgaccagttt gccggaacgg tgggcagacc
     8041 gttttcgact tcgctttcga cataaatcag ggtttcgtca gccagccagc cgttatcttc
     8101 cagtaaattt atcgtctctt ctaacaaacc acggcggaac ggtggatcga caaacacgat
     8161 attatgcggt gtgccttttt gcgccaggaa tgacattgcg ttgctgttca ccacgcgtgc
     8221 attgcctgct tttagtgtcg ccagattctt aattaactgc tgagaaactg cacgatccat
     8281 ctcaattaac gtggcccccg cagcgtagcg cgataacgct tccagcccca gcgcgccgct
     8341 cccggcgaag caatccagac attgggcgtc aacaatgacc ggagccagcc agttaaacaa
     8401 cgtttcgcgt acgcggtcgg tggtggggcg cagacctggg ctatcaggaa ccgggagttt
     8461 acggcctcgc cactgcccgc caataatgcg gatttggccg ctgccggaat gattcggttt
     8521 tttcatgata aattgctcaa tccgccagat aacacatact tgcaggcggt gatgtgaatt
     8581 aagttaagtg atagactatt tcatcatttt tttagctgct atgtacatag cgttaacgct
     8641 gtgccatgaa gcaacagcga ggagtgtagt cgcaaatggc gaaagaaaaa aaacgtggct
     8701 ttttttcctg gctgggcttt ggtcaaaaag agcagacccc ggaaaaagag acagaagttc
     8761 agaatgaaca aacggttgta gaagaaatcg ttcaggcgca agagcctgtg aaggcttctg
     8821 aacaagccgt cgaagagcag ccgcaggcgc atactgaggc cgaggcggaa acttttgctg
     8881 ccgacgttgt ggaagtcact gaacaggttg ctgaaaatga aaaagcgcag cctgaagcgg
     8941 aagtcgttgc acaaccggaa ccggtcgtag aagaaacgcc ggagccagtg gctatcgaac
     9001 gtgaagagct gccgttgccg gaagacgtca acgccgaaga ggtttcgcca gaagagtggc
     9061 aggctgaagc ggaaaccgta gagattgtcg aagcggcgga agaagaagcg gctaaagaag
     9121 aaatcaccga cgaagagctg gaagcacagg cgctggctgc cgaagcagca gaagaggcgg
     9181 tgatggtggt tcctccggca gaagaagagc agccggtgga agaaatcgct caggagcagg
     9241 aaaaaccgac caaagaaggt tttttcgcgc gcctgaaacg cagcctgtta aaaaccaaag
     9301 aaaatctcgg ttccggattt atcagcctgt tccgcggtaa aaaaatcgac gatgatctgt
     9361 ttgaggagct ggaagagcag cttttgatcg ccgatgtggg cgtggaaacc acacgtaaaa
     9421 ttatcaccaa tctgacggaa ggcgcatccc gcaagcagct tcgtgatgcc gaggcgctct
     9481 atggcctgct gaaagaagag atgggtgaga ttctggcgaa agtcgatgag ccgctgaatg
     9541 ttgaaggtaa aacgccgttc gtgatcctga tggtgggcgt caacggtgtg ggtaaaacca
     9601 cgacgattgg taagctggca cgtcagtttg agcagcaggg taaatcggtg atgctggcgg
     9661 cgggcgatac tttccgtgca gcagcggttg aacagcttca ggtctggggg cagcgcaaca
     9721 atattccggt gattgcccag cataccgggg cggattccgc ctctgttatc ttcgacgcca
     9781 ttcaggcggc taaagcgcgt aatatcgacg tcctgattgc cgataccgcc ggacgcctgc
     9841 agaacaaatc gcacctgatg gaagagttga agaaaatcgt ccgcgtgatg aagaaactcg
     9901 acgttgaagc gccgcatgaa gttatgctga ctatcgatgc cagcaccggg cagaacgcgg
     9961 taagccaggc caaactgttc catgaagccg ttggcttaac cggcatcacg ctaacgaaac
    10021 tggacggcac ggcgaaaggc ggggtaattt tctcggtggc tgaccagttt ggtatcccta
    10081 tccgctacat tggtgtcggc gaacgtattg aggatttgcg tccgtttaag gcggacgact
    10141 ttatagaggc actttttgcc cgagaggatt aacaatgatt cgctttgaac atgtcagcaa
    10201 ggcttatctc ggtgggagac aggcgctgca gggcgttacg ttccatatgc agccgggtga
    10261 gatggcgttt ctgaccggtc attccggcgc agggaaaagt accctcctga agctgatctg
    10321 tgggattgag cggcccagcg ccgggaaaat ctggtttagc ggccatgaca tcacgcgtct
    10381 gaaaaaccgt gaagttccgt ttctgcgccg ccagattggc atgattttcc aggatcacca
    10441 tctactgatg gaccgtactg tctacgataa cgtggcgatc ccgctgatta tcgccggtgc
    10501 cagcggtgac gatattcgcc gccgggtgtc ggcggcactg gataaagtcg ggctactgga
    10561 caaagcgaag aacttcccta ttcagctttc tggcggtgaa caacagcgtg ttggcattgc
    10621 ccgcgcggtg gtgaacaagc ccgcggtact gctggcggac gaaccgactg gtaacctgga
    10681 cgacgcgttg tcggaaggga ttctacgtct gtttgaagag tttaaccgcg taggggtaac
    10741 cgtattgatg gcaacgcacg acatcaacct gatttcacgg cgttcctatc gcatgctcac
    10801 cctgagcgat ggtcacttgc atggaggcgt gggccatgaa taagcgcgat gcaatcaatc
    10861 atattcggca gtttggcggg cgtcttgatc gcttccgtaa atcggtcggc ggctcaggcg
    10921 acggcggtcg taacgcaccg aaacgcgcga aatcctcgcc aaaaccggta aatcgcaaaa
    10981 ccaacgtttt caacgaacag gtgcgctatg ccttccacgg cgcattgcag gatctgaaaa
    11041 gcaaaccgtt cgccacattt ttaacggtga tggttatcgc catctccctg acgctgccca
    11101 gcgtctgtta tatggtgtac aaaaacgtta accaggcggc gacgcagtat tatccgtcac
    11161 cgcaaatcac tgtttatctg caaaaaacgc tggacgatga cgccgctgcg ggcgtggtgg
    11221 cacagttgca ggccgagcaa ggcgtagaga aagtgaacta tctttctcgt gaagacgcac
    11281 tgggcgagtt ccgtaactgg tctggttttg gtggcgcgct ggatatgctg gaagaaaacc
    11341 cgcttccggc agtggcggtg gtgatcccga agctcgactt tcaggggacg gagtctctga
    11401 atacgctgcg tgatcgtatc acgcagatta acggcattga cgaagtgcgg atggatgaca
    11461 gctggtttgc ccgtctggcg gcgttgaccg ggctggtcgg gcgcgtttcg gcgatgatcg
    11521 gcgtgttgat ggtggcggca gtgttcctcg tcatcggtaa cagtgtacgc ctgagtatct
    11581 ttgctcgtcg tgactccatt aacgtgcaga aactgattgg tgcaacggat ggtttcatcc
    11641 tgcgcccgtt cctgtacggt ggcgcattgc tggggttttc tggcgcattg ttgtcattaa
    11701 ttttatcaga aattctggta ctgcgattgt catcggcggt tgcggaagtg gcacaggttt
    11761 tcggaacgaa gtttgatatc aatggtttat cgttcgatga atgcctgctg ttgctgctgg
    11821 tatgctcgat gattggctgg gtggcagcgt ggcttgccac ggtacaacat ttacgccact
    11881 ttacgcctga ataataaaag cgtgttatac tctttccctg caatgggttc cgtagcaggg
    11941 aaagagaccc cgttgtctct tcccggtatt tcatctctat gtcacatttt gtgcgtaatt
    12001 tattcacaag cctgcattga acttgtggat aaaatcacgg tctgataaaa cagtgaatga
    12061 taacctcgtt gctcttaagc tctggcacag ttgttgctac cactgaagcg ccagaagata
    12121 tcgattgaga ggatttgaat gactgacaaa atgcaaagtt tagctttagc cccagttggc
    12181 aacctggatt cctacatccg ggcagctaac gcgtggccga tgttgtcggc tgacgaggag
    12241 cgggcgctgg ctgaaaagct gcattaccat ggcgatctgg aagcagctaa aacgctgatc
    12301 ctgtctcacc tgcggtttgt tgttcatatt gctcgtaatt atgcgggcta tggcctgcca
    12361 caggcggatt tgattcagga aggtaacatc ggcctgatga aagcagtgcg ccgttttaac
    12421 ccggaagtgg gtgtgcgcct ggtctccttc gccgtccact ggatcaaagc agagatccac
    12481 gaatacgttc tgcgtaactg gcgtatcgtc aaagttgcga ccaccaaagc gcagcgcaaa
    12541 ctgttcttca acctgcgtaa aaccaagcag cgtctgggct ggtttaacca ggatgaagtc
    12601 gaaatggtgg cccgtgaact gggcgtaacc agcaaagacg tacgtgagat ggaatcacgt
    12661 atggcggcac aggacatgac ctttgacctg tcttccgacg acgattccga cagccaaccg
    12721 atggcaccgg tgctctatct gcaggataaa tcatctaact ttgccgacgg cattgaagat
    12781 gataactggg aagagcaggc agcaaaccgt ctgaccgacg cgatgcaggg tctggacgaa
    12841 cgcagccagg acatcatccg tgcgcgctgg ctggacgaag acaacaagtc cacgttgcag
    12901 gaactggctg accgttacgg tgtttccgct gagcgtgtgc gccagctgga aaagaacgcg
    12961 atgaaaaaac tgcgcgctgc tatagaagcg taatttccgc tattaagcag agaaccctgg
    13021 atgcgagtcc ggggtttttg ttttttgggc ctctacaata atcaatttcc cctccggcaa
    13081 aacgccaatc cccacgcaga ttgttaataa actgtcaaaa tagctattcc aatatcataa
    13141 aaatcgggta tgttttagca gagtatgctg ctaaagcacg ggtagtcatg cataaaacga
    13201 aataaagtgc tgaacaacaa catcacaaca cacgtaataa ccagaagaat ggggattctc
    13261 aggatgaaca taaagggtaa agcgttactg gcaggatgta tcgcgctggc attcagcaat
    13321 atggctctgg cagaagatat taaagtcgcg gtcgtgggcg caatgtccgg tccggttgcg
    13381 cagtacggtg accaggagtt taccggcgca gagcaggcgg ttgcggatat caacgctaaa
    13441 ggcggcatta aaggcaacaa actgcaaatc gtaaaatatg acgatgcctg tgacccgaaa
    13501 caggcggttg cggtggcgaa caaagtcgtt aacgacggca ttaaatacgt gattggtcac
    13561 ctctgttctt catcaacgca gcctgcgtct gacatctacg aagacgaagg cattttaatg
    13621 atcaccccag cggcaaccgc gccggagctg accgcccgtg gctatcagct gatcctgcgc
    13681 accaccggcc tggactccga ccaggggccg acggcggcga aatatattct tgagaaagtg
    13741 aaaccgcagc gtattgctat cgttcacgac aaacagcaat acggcgaagg tctggcacga
    13801 gcggtgcagg acggcctgaa gaaaggcaat gctaacgtgg tgttctttga tggtatcacc
    13861 gccggggaaa aagatttctc aacgctggtg gcgcgtctga aaaaagagaa tatcgacttc
    13921 gtttactacg gcggttatca cccggaaatg gggcaaatcc tgcgtcaggc acgcgcggca
    13981 gggttgaaaa ctcagtttat ggggccggaa ggcgtggcta acgtctcgct gtctaacatt
    14041 gcgggcgaat cagcggaagg gctgctggtg accaagccga agaactacga tcaggttccg
    14101 gcgaacaaac ccattgttga tgcgatcaaa gcgaaaaaac aggacccaag cggcgcattc
    14161 gtttggacca cctacgccgc gctgcaatct ttgcaggcgg gcctgaatca gtctgacgat
    14221 ccggctgaaa tcgccaaata cctgaaagcg aactccgtgg ataccgtaat gggaccgctg
    14281 acctgggatg agaaaggcga tctgaaaggc tttgagttcg gtgtgtttga ctggcacgcc
    14341 aacggcacgg caaccgatgc gaagtaacag atagataaat gaaaatgccc gatgcgctga
    14401 actgccatcg ggtttttttg tgtttgtcga aatggttgta gaaagattaa acaatccagg
    14461 atatgcttaa tgtacggtga cataccgtac atattatacg gagcaggaga gttcacatgt
    14521 ctgctgttaa aaagcagcgt atcgatctgc gtttaactga cgatgacaaa agtatgattg
    14581 aggaagcggc agcgatatct aatcagtccg tcagccaatt tatgttgaac agcgcttcgc
    14641 agcgggctgc ggaagtgatt gaacagcatc ggcgggtgat cctcaatgag gaatcctgga
    14701 cgcgggtgat ggatgcgctg agtaatccac cgtcaccagg tgaaaagcta aaacgtgcgg
    14761 caaaacgtct tcagggaatg taataattta tggatgatct gacgatagag attctgaccg
    14821 atgatgcaga ttatgatcta cagcgattcg actgcggcga ggaagcgtta aatctctttc
    14881 tgacgacaca tctcgttcgt caacatcgca acaaaattct gcgagcgtat atcctttgtc
    14941 gcaacactcc agaacgtcag gtgctgggat attacacatt atgcggcagt tgttttgaac
    15001 gagccgcatt gccctcgaaa tcgaaacaga aaaaaattcc ctacaaaaat attcccagcg
    15061 ttactcttgg gcgtctggca attgatcgtt cattacaggg gcagggatgg ggagcaacac
    15121 tggttgctca tgccatgaaa gtcgtctggt cagcctcttt agcggtaggt attcacggtc
    15181 tttttgtcga ggcgctgaat gaaaaagccc atacgtttta taaatcgctg ggctttatcc
    15241 ctttagtcgg ggaaaacgaa aatgcgttat ttttcccaac caaatccatt gaactgcttt
    15301 ttacacagag cgattaacgc ttctcccagc cgccctgctg tgccgtaaat cccagtgcct
    15361 gcataaacgc cgtcatcaca ccgcgatctt ccacgcctgc atccgccatc caccagcatg
    15421 aaacgccagg attgttacgc aaaacctctt ccagcagata ttgccccaca ccgcgacgtc
    15481 gagtgacttc ccgcacgcgc agggaatcca gtgccccctc ggtgccgctt aaggttaccc
    15541 gcacggcagc aagcaggcgc tcgttaaaac gcgcggcgta gatgcggtgg ttatcgtcaa
    15601 cctgtaaaga ggaaggggaa tactccggcc agatcttttg caggtcaatc cggtcctggt
    15661 cgctaaattt ttctaatcga atgatggtca gcttcatggg taacccgtgt aaatcacaaa
    15721 agtggaacca gtgtagcgaa ataatttaat cggaggcttt ctc
//
LOCUS       DBFIFI010000095        15364 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.095, whole genome shotgun sequence.
ACCESSION   DBFIFI010000095 DBFIFI010000000
VERSION     DBFIFI010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15364
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.095"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            89..361
                     /locus_tag="N7B39_002857"
     CDS             89..361
                     /locus_tag="N7B39_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HGU2422811.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(362..1234)
                     /gene="rluF"
                     /locus_tag="N7B39_002858"
     CDS             complement(362..1234)
                     /gene="rluF"
                     /locus_tag="N7B39_002858"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HGU2422812.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1582..2529
                     /locus_tag="N7B39_002859"
     CDS             1582..2529
                     /locus_tag="N7B39_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313031.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HGU2422813.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTNISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGEMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            2599..3405
                     /locus_tag="N7B39_002860"
     CDS             2599..3405
                     /locus_tag="N7B39_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU2422814.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEIADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            3415..3822
                     /locus_tag="N7B39_002861"
     CDS             3415..3822
                     /locus_tag="N7B39_002861"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HGU2422815.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQNEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            3822..4316
                     /locus_tag="N7B39_002862"
     CDS             3822..4316
                     /locus_tag="N7B39_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HGU2422816.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIYKINEQ
                     LIAN"
     gene            4382..5179
                     /locus_tag="N7B39_002863"
     CDS             4382..5179
                     /locus_tag="N7B39_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HGU2422817.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLLAPASTALDQLDD"
     gene            5190..6014
                     /locus_tag="N7B39_002864"
     CDS             5190..6014
                     /locus_tag="N7B39_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HGU2422818.1"
                     /translation="MEQRKITRGDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPVKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VTMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            6066..7295
                     /gene="sorE"
                     /locus_tag="N7B39_002865"
     CDS             6066..7295
                     /gene="sorE"
                     /locus_tag="N7B39_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HGU2422819.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRMLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLTHAEAISHD"
     gene            7678..8367
                     /gene="pepE"
                     /locus_tag="N7B39_002866"
     CDS             7678..8367
                     /gene="pepE"
                     /locus_tag="N7B39_002866"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HGU2422820.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(8458..10089)
                     /locus_tag="N7B39_002867"
     CDS             complement(8458..10089)
                     /locus_tag="N7B39_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HGU2422821.1"
                     /translation="MLTLLNLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLAMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMSSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(10309..13992)
                     /gene="metH"
                     /locus_tag="N7B39_002868"
     CDS             complement(10309..13992)
                     /gene="metH"
                     /locus_tag="N7B39_002868"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HGU2422822.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWEVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            14192..15016
                     /gene="iclR"
                     /locus_tag="N7B39_002869"
     CDS             14192..15016
                     /gene="iclR"
                     /locus_tag="N7B39_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HGU2422823.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         3654 a         4019 c         4019 g         3672 t
ORIGIN      
        1 gttaagagaa ggcaaaatga atattatcag ttctgctcgc aaacgaattc ccgtacactg
       61 aactctctgg ctcactgagg gcaccatcat ggcactcccc cgtattaccc aaaaagagat
      121 gactgaacgc gagcagcgtg aacttaaaac actgctggat cgggcgcgga ttgcgcatgg
      181 tcgcgtactg accaactccg aaaccaacag cattaagaaa gagtacatcg ataaattaat
      241 ggtcgagcgt gaggctgaag cgaaaaaagc ccgccagtta aagaaaaagc aggcttataa
      301 accggatcct gaagcgtcat ttagctggtc agcaaatacg tcgacgcgcg gcaggcgctg
      361 attagcgccc cttctttttt cgccccggcg aggtaaaacg cttaccgttg gacgccgggc
      421 gaccgccttt ttccgccgtt ttttccatct taacgactgg acgtttgatg cccgctgttt
      481 tcggtttggc cttcgcttta ggttttgcct cggaagagga attttcgatg agcttaaaga
      541 ggtcgattaa ctcatcgtcg gttaaatcgc gccactcccc cagcggaatg ccgcttaagc
      601 tgacgttcat aatgcgcgta cgttccagct ttttcacttc atagccgaaa tgctcgcaca
      661 tgcgccggat ctgacggttc agcccctgca ccagggtaat gcggaagaca aacggcgctt
      721 cttttttgac tttgcacttt ttagtcaccg tcccgaggat cggcaccccc gcccccatac
      781 cacgaataaa ttcatcggta atcggtttat cgaccgtcac cagatactct ttctcatgat
      841 cgttacccgc acggaggatc ttattcacca gatcgccgtg attggtgagg aaaatcagcc
      901 cctgggagtc tttatccagg cggccaatcg ggaacacgcg tttgctgtgg ttaacgaaat
      961 cgacaatgtt atcgcgctcg ccatcttcgg tggtgctgac aatgcctacg ggcttgttca
     1021 gggcgataag taccaaatct tcggcttccc gaggctcaat caactgaccg tttactttca
     1081 caacgtcgcc gggtttcacc tgatcgccaa tggtggctcg cttgccatta aggaacacat
     1141 tgccttgctc gatatagcga tccgcttcgc ggcgtgagca aattccgctt tcgctgatgt
     1201 atttatttaa acggactgat gagtcgggca gcataaattc tcctgtaaaa cgcggaatat
     1261 accgtacctc ttagcgggga aaaacagggt tatgcggcaa cgtcgaaatt aaatgtgatc
     1321 tgccacgcct tttatcacaa aagtgtctcc ggaagtacaa tgcaaatttg cgtggcaaaa
     1381 taaggataat caacatatta cgaatcgctg cacacctgtg ctgataacta ctgcgcagag
     1441 aaacaaatgt gcaagagaaa gcgctatttc tccccatgca aagagtgaaa aatcgctttg
     1501 aaaggccatc atgatggggt tatatcagat caagcggtga gaaagcgtat ttgcacaaat
     1561 gtgcagaacc aggaggcggc aatggaaaac agtgacgata tccgtttgat tgtgaagatt
     1621 gcccaactct attacgaaca ggatatgacg caggcgcaaa tcgcgcgcga actgggtatt
     1681 taccgcacca acatcagccg cttgcttaaa cgaggccgcg atcagggaat tgtcaccatc
     1741 gccatcaact atgactacaa cgaaaatctc tggctggagc agcaactgaa gcaaaagttt
     1801 ggcctgaaag acgttgtggt ggtgtcggga aatgatgagg atgaagagac tcaactggcg
     1861 atgatggggt tacacggcgc gcaactgctg gatcgcttgc tggagcctgg cgatattgtc
     1921 ggtttttcct ggggtcgcgc ggtgagcgca ctggttgaaa acttgccgca ggcggggcaa
     1981 tcgcggcagt taatctgcgt accaattatt ggcggaccgt ccggtaaact cgaaagccgc
     2041 tatcacgtaa acacattaac ctacagcgcg gcagcgaagc tgaaagggga atcgcatctc
     2101 gcggattttc cggctctact ggataaccca ttaattcgta atgggatcat gcagtctcag
     2161 cactttaaaa ccatctctgc ctactgggat aatctggatg tcgccctggt gggaattggc
     2221 tcaccggcca ttcgcgacgg cgctaactgg catgcgtttt atggtggtga agagagtgac
     2281 gacctgaatg cccgccaggt tgctggcgat atttgctcgc gcttttttga tattcacggc
     2341 gaaatggttg aaacgaatat gagcgaaaaa acactctcta tcgaaatgaa taaattaaag
     2401 caggcacgat attccattgg cattgccatg agtgaagaaa aatacagcgg aattattggt
     2461 gcactgcgtg gaaaatatat taattgtctg gtaacgaata gcagcacagc tgaactatta
     2521 ctgaaataaa aatcttcccg atgtttatcg taataaaaat tacggcgggt ccctattatc
     2581 taaaatcagg agtctgttat gcaaacgtgg ttaaatttgc aggataaaat cattatcgtc
     2641 accggcggcg catccggtat tggtctggcg attgtagagg aattattagc acaaggcgca
     2701 aacgtacaga tggtagatat tcacggtggc gatggacaat atgaaggtca taaaggctat
     2761 cagttttggc cgaccgatat ttccagcgcc aaagaggtaa atcatacggt agcggaaatt
     2821 atccagcgtt ttggtcgcat cgacggtctg gtcaataacg ccggggtcaa tttcccgcgt
     2881 ctgctggtcg atgagaaagc gcctgccggg cagtatgaac tcaacgaagc tgcattcgaa
     2941 aaaatggtca atatcaacca gaaaggcgtt tttctgatgt cgcaggcggt ggcgcgacag
     3001 atggtcaaac aacatgatgg cgtgattgtg aatgtttcct cagaaagtgg gctggaaggc
     3061 tcagaaggcc aaagctgtta cgccgctacc aaagccgcgc tcaatagctt cacgcgctcc
     3121 tggtcgaaag agctgggtaa acacggtatc cgtgtggtcg gtatcgcgcc ggggattctg
     3181 gaaaaaaccg ggctacgcac gccggaatat gaagaggcgc tggcgtggac gcgcaatatc
     3241 accgtcgagc agctgcgtga aggctatacc aaaaacgcca ttcctattgg gcgcgccgga
     3301 agattagcag aaatcgctga ttttgtttgt tatctgctgt ctgaacgcgc cagctatatc
     3361 accggagtaa ccactaacat tgcgggcggc aaaacgcgcg gctaaggagg ctttatggtc
     3421 aacgccattt tttgtgccca cggcaaatta gcttgcgcca tgctggaatc ggttcagatg
     3481 gtttacggca acgccaatgt tgaagccgtt gaatttgtgc ccggtgaaaa cgccggagac
     3541 atcgtagcaa agctggaaaa attagtaagc attcacaatc aaaatgagtg gctgattgcc
     3601 gtggatttac agtgcggcag cccgtggaat gcggcagcga tgcttgcgat gcgcaatcct
     3661 cgtctgcggg taatcagcgg actttctctg ccgctggcgc tcgaactggt tgataaccag
     3721 gacagcatga atgttgatga actgtgtgaa cacctgacgc aaatcgcaaa gcaaacctgc
     3781 gttgtctgga aacaactggc aacgacagag gaggatttct gatgaacatt acgctcgccc
     3841 gcattgatga ccgcctgatc cacggtcagg tcactaccgt ctggtcgaaa gtggctaacg
     3901 cccagcgcat tattatctgc aatgacgaag tttataacga tgaagttcgc cggacattat
     3961 tgcgtcaggc tgcgccaccg ggaatgaagg ttaacgtggt caatattgaa aaagccgtcg
     4021 cggtttatca caacccgcaa tatcaggacg aaaccgtttt ttatcttttc actcgcccac
     4081 aggatgcctt agcgatggtt cgccaggggg taaaaatagg caccctgaat attggcggca
     4141 tggcctggcg gcccggtaaa aaacagttaa ccaaagcggt ttcattagat gatgatgata
     4201 ttaacgcatt tcatgagtta aataatctag gcgtcatact ggatttacgc gtcgtagcgt
     4261 cagatccctc aatcaatatt atctacaaaa taaacgaaca gttaatcgca aattaaataa
     4321 tagcgcctgt gtacatatgc tttaacaggc agggatgacg acacttaaaa ggtgacaaat
     4381 catggaaata agtaccctac aaatcattgc tatatttctt ttttcctgta ttgccggaat
     4441 gggcagcgtg ctggatgaat ttcagactca tcgcccgtta attgcctgta cggtgattgg
     4501 tttaattctc ggtgatttaa aaaccggaat aatactcggt ggtacgctgg aattgatagc
     4561 tctcggctgg atgaacgtcg gcgcggcgca atctccggat tctgcactcg ccagcataat
     4621 ctccgccatt ctggttatcg ttggtcagca aagcatcgcc accggaatcg ccatcgcgtt
     4681 gcctgtggct gcggcaggcc aggtgctgac ggtgtttgcc cgtaccatca ccgtagtgtt
     4741 ccagcacgcg gcggataaag cagcggaaga agcacggttt cgcactctcg atattctgca
     4801 tgtctccgcg cttggcgtgc aggcgctgcg cgtcgccatt ccggcactga ttgtctcgct
     4861 gttcgtcagc gccgatatgg tgagcaatat gttaaacgcc attcccgaat ttgtgacccg
     4921 aggtctgcaa attgctggcg gttttatcgt ggtagtcggt tacgccatgg tgctacgtat
     4981 gatgggcgtg aaatatttga tgcctttctt tttcctcggt ttcctcgcag gtggctacct
     5041 cgatctcagt ctgctggcgt tcggtggcgt cggcgtgatc atggccctgg tctacatcca
     5101 gctaaatcca cagtggcgta aagctgaacc tcaactcctg gcccccgctt ccaccgccct
     5161 tgaccaactt gacgattaac ggagccatca tggaacagag aaaaattaca cgcggcgatc
     5221 tggtgagcat gtttctgcgc tccaacctgc aacaggcgtc ctttaacttt gaacgtattc
     5281 acgggctggg tttttgctac gacatgatcc ccgccatcaa gcgactttac ccagtaaaag
     5341 aggatcaggt tgcggcgctc aggcgacacc tggtgttctt taataccacg ccagccgtat
     5401 gtggcccggt catcggcgtc actgccgcca tggaagaggc gcgggccaac ggcgcggaaa
     5461 ttgatgacgg caccattaac ggcatcaaag tcggtctgat gggaccattg gcaggggttg
     5521 gcgatccact ggtctgggga acgctgcgcc cgattaccgc cgcgctcggc gcatcgctgg
     5581 cgctgtctgg caacattctc ggcccgctgc tgttcttctt tattttcaac gcggtgcgtc
     5641 tggcgatgaa gtggtatggc ctacagctcg gctttcgcaa aggggtgaat atcgtcagcg
     5701 atatgggcgg gaatgtgctg caaaaactca ccgaaggcgc gtcgattctc ggactgtttg
     5761 tgatgggcgt gctggtcacc aaatggacgt caatcaacgt accgttggtg gtttcacaaa
     5821 cgcatgccgc cgatggctcc accgtcacca tgaccgtgca gaacattctc gatcaacttt
     5881 gccctggttt gctggcgctc ggtctgacgc tactgatggt tcgtctgctc aacaaaaaaa
     5941 ttaacccggt atggctgatt ttcgccctgt ttggcttagg gattatcggc aatgcgctgg
     6001 gcttcctgtc ctgattcttc gccccggcac gactgccggg gccatcgttc aacatgaggt
     6061 ggtttatgaa aacaacagct ctgcgtcttt atggtaaacg tgatttacgc ctggaaacct
     6121 ttgaccttcc tgaaatgcag gaggatgaaa tcctcgcgac ggtggtcact gacagcctgt
     6181 gcctctcttc ctggaaagag gccaatctgg gtgaaaacca taaaaaagta cccgacgatg
     6241 tggcgaccaa cccaatcatc atcggccacg agttttgcgg cgatattctg gccgtgggta
     6301 aaaagtggca gcacaaattc cagccgggtc agcgttatgt gattcaggcc aacctgcaac
     6361 tccccgaccg cccggactgc cccggctact ccttcccgtg ggtaggcggc gaggccacgc
     6421 atgtggttat tcccaacgag gtcatggaac aagattgcct gctggcatac gacggcgaaa
     6481 cctattttga aggctcgctg gttgaaccgc tttcctgcgt gattggcgcg ttcaacgcca
     6541 actatcatct tcaggaaggt agttataacc acacgatggg gattcgcccg caagggcgca
     6601 tgctgatcct cggcggcacc ggaccaatgg gactgttggc gattgattat gcgctacatg
     6661 gacccgttaa cccgtcgctg ctcgtcatta ccgataccga caacgataaa ttgagttatg
     6721 cgcgcaagca ctatccatca gaaccgcaaa cactgattca ttatctcaat gccgccgatg
     6781 cagcatttga tacgctaatg gcgctgagtg gcggtcacgg cttcgatgat attttcgtct
     6841 ttgtgcctaa tgaaggactg gtgactctcg cctcttcctt gctggcgaca gatggttgcc
     6901 tgaatttctt cgccggaccg caggataaac atttcagcgc gccaattaat ttctacgatg
     6961 tgcattatgc atttacccac tacgtgggca cgtcaggcgg caataccgac gacatgcgcg
     7021 cagcggtcaa attgattgaa gagaaaaaag tgcaggccgc aaaagtggta acacatattc
     7081 ttgggctgaa tgccgcgggc gaaaccacgc ttgaattgcc tgccgtcggc ggcggcaaaa
     7141 agctggtgta taccgggaaa tacctgccgc tgacgtcact cacgcagatt caggatcaag
     7201 cactggcggc gattctggcg cgtcatcagg ggatctggtc cggtgaggcg gagcaatatc
     7261 tgctcactca tgcagaggca atttcccatg attaatcgcg ctacttaact ctgcatgtcg
     7321 ctggctggtc accgcggcaa cttcgcgacc tgatgtgcgg ctgattgctg aagcggccca
     7381 atgtgcacga gaaggataaa aaaacatcag cgccttaagg ggcgctcaaa ttggtggcaa
     7441 agtacacatt gtttatgccg gatgcggaat aaagccttat tcggcctaca taatctcgaa
     7501 aattcaatat attgcagtta tctggtaggc ctggtaagcg tagcgcatca ggcaatgctg
     7561 tgtttgtcat cagtctcagc gcccggtgca atacgtgaca tcgtgagcca tgtcagtaag
     7621 tggttaaatt cgcagtggta gcatgccttc cgtgatttct tactaaccgg agaagtaatg
     7681 gaactgcttt tattgagtaa ctcgacgctg ccgggtaaag cctggctgga acatgcactg
     7741 ccgctgattg ctgaacagtt gcagggtcgc cgctcagcgg tgtttatccc tttcgctggc
     7801 gtaacgcaga cctgggatga ttacacagcg aaaacggctg cggttctcgc tccgctgggt
     7861 gtttctgtca ccggtattca tagcgttgtc gatcccgttg ccgcgattga aaatgcggaa
     7921 attgtgattg tcggcggcgg taatactttc cagttgctga aacagtgccg cgagcgcggg
     7981 ctgctggctc caattactga cgtggttaaa cgtggcgcgc tgtatattgg ctggagcgca
     8041 ggcgctaacc ttgcttgccc aaccattcgt accaccaacg atatgccgat tgtcgatccg
     8101 caaggtttcg atgcgctaaa tctgttcccg ttgcaaatca acccgcactt caccaacgcg
     8161 ctgccggaag gccataaagg tgaaacccgt gagcagcgta ttcgcgaact gctggtcgtc
     8221 gcgccagaac tgacgattat tggtctaccg gaaggtaact ggatcacagt gagtaaaggt
     8281 cacgcaacgc tgggtggccc gaataccacc tatgtgttta aggccggtga agaagcggtt
     8341 ccgctggaag ctggtcaccg tttttaagtg aattgccgga tgcatgatcc ggcatcttgt
     8401 tcatagtgcc tgatgcgacg cttgacgcgt cttatcaggc ctacaggatt cacaatatta
     8461 atactcgtcc cgtccctcat cttcatcagg ctgttccagc acactgtaag ccaccgaaca
     8521 aaacagcgag ttcagacgct gcatatctcc cagtaagcct aaatgtagcg aactggtttc
     8581 aatgctttgc acgttttgct gatgcaggcg atcgacgtgg gcgtgggaat agcggcgatt
     8641 aagaatgcga aagcgatgct tgctgcggcg caaacgacga gcgctggtga catcgccaga
     8701 gaagaacacc gacattgcca gctttaaatt actgagcaat tgctcataga gcgcatccag
     8761 ttccttcaag ccatcaagcg aaaatgctcg ccttgcagcc agcgatttgt cggcaatttc
     8821 actactcatg cgctcgacga tatcggaggc ctgttcaagg ttgagcgaca tttcgatgat
     8881 ctccgcccag cggcgcgact cttcttctgc cagttcctct tttggcatcc gcgccagata
     8941 cagcttaatg gcggtataga gaacgttgat atcatctgcc agcttacgca gctctttctc
     9001 ctgccttggc tcgccgtgca tcactttatt cagcccttcc atcatctgtt ccatggcgtc
     9061 gccaatgcgc agggtttcgc gcgcggcgtt cgccagagca agcgtgggcg tatccagcgc
     9121 gctgacatcc agatgtttag gccgtagctg ggtatccagt tccggttcat cgcgaataat
     9181 cgttttgcaa aaccgtgcca tcgggtcaac aaatggcagc atggccaggc aacgtacaag
     9241 gttgtagaag acgtggaaat agatcaccag ttccgctttt ggcaatgaca acttccccat
     9301 tgtctctgcc agcaaatgga caaacggcag gataatcagg ctacccacca gcttaaacag
     9361 cagactaccc agcgcgacac ggcgggctgc ggcattggcg gcactgttgt tgagcatcgc
     9421 cagcaggccg gaaccgaggt tagcaccaat caccagacag agcgccacgg ggaaggagat
     9481 aatgcctgcg gcggtcagag tcgcggtcag cagtacagca gcaaggctgg agtagctgat
     9541 aatggcgaac atcgcgccaa tcagcgcatc cagcagaata tcgccggtca gcgaggcaaa
     9601 gatcacctga acgccgtttg cctgggtgat cggcgttacg gcctgcacaa tcaactccag
     9661 cgccagcaaa atcagcccaa gaccaatacc gacgcggccc agttgcccgg cgcgtgactg
     9721 tttgcgtccg aggaaaaaaa tcacgccgat aaaaataagt aacggtgaca gccaggataa
     9781 gtcgaaggtg agaatacgcg ccattagcgc cgtcccgaca tctgccccca gcacaatgac
     9841 cagagccggt gcgagggcta ccagatcctg agcgacgaac gaggtcacca gcatggtggt
     9901 ggcattactg ctctgtacca gtgcggtaac gccgatcccc gcgcaaaagg cgagcggctt
     9961 tttttcgacg ctccggctaa ggacagtacg caaacgcgcg ccgaagacgc gcattacgcc
    10021 ggttcgaaca atatgagtcc cccagaccag cagggcgacg gcagaaagca ggttaagcag
    10081 cgttaacacg agttcaggtt ctccttatcg ttatatgttc ctgaagcaat tgaatgacca
    10141 aaggaccgga aacacgctga ctggcatctt tactgtgcct tttgacattc gtttttccgg
    10201 ctaagattat tccagcctgt ttccagtata agtgtttcat attaataaag agacagggca
    10261 ctcattgagc gccctgtttg ttgtaaggaa aagtgacaga tttgtgaatc agtccgcgtc
    10321 ataccccaga ttcggtgcca gccagcgctc aacatcggag acgctcatac ctttacggcg
    10381 ggcatagtct tcaacctgat cgcgctgaat ttgcgccacc gcgtagtact tgctgtccgg
    10441 gtggctgaaa taccaacccg aaaccgatgc accgggccac atggcgaaag attctgtgag
    10501 tttcatgccg gtatgttttt ccacttccag cagctcccag atggtggctt tttccgtatg
    10561 ttccgggcag gccgggtagc ccggtgccgg acggatgccc tggtagtttt cgcggatcag
    10621 ttcttcgttg ctgaggttct cgttcggcgc atagccccag tagactttac gcacacgctc
    10681 atggagatac tccgcaaagg cttcggctaa acggtcggca agcgctttca ccatgatttt
    10741 gttgtaatca tcgtgctgcg cttcaaaggc atcagccagt gcgtcctctt ccagcccgcc
    10801 agtcacggca aatgcgccga tgtaatctgc tttaccagaa agcttcggcg caacgaagtc
    10861 agcgagacag tagttagcga atcccgtttt ttcggtctgt tgacgcagat ggtggctgac
    10921 gttgatcaca tgggtgcgcg tttcgtcacg gtagatttca atgtcatcgc ccacacggtt
    10981 tgccgggaac aggcccacca cgccacgcgg attcagcgtt ttctcggcgc ttaatttatc
    11041 cagcatgtcg ttggcgtctt taaacagccg ctgcgcctca acgcccacca cttcatcttc
    11101 cagaatgcgc ggatacttcc cggccagcga ccaggtcata aagaacggtg tccagtcgat
    11161 gtaattacgc agagtttcga tgctggcttc aacttcctgc acgccgagac ggtgcgccac
    11221 cggcggcgtg taagcctgcc agtcaaaagc gaagtcgtta tcgcgcgccg cttccagcgt
    11281 gaccggtggt gtgcgtggtt tcttgcgccc gtgctgaata cgtacggttt cgtactcctt
    11341 gcgggtacga gcgacaaaat catcgcgctg agtatcggaa agcagcgccg ccaccacacc
    11401 aacggtgcgc gaggcattct gcacatacac cgtcgggccg ctgtagttct gctcgatttt
    11461 caccgccgtg tgcgcttttg aggtcgtcgc gccaccgatc agtaacggaa tggtgaagcc
    11521 ctgacgctcc atctctttcg ccacgttaac catctcgtcc agcgacggcg tgataagccc
    11581 cgaaagtcca atcagatcag cattcacttc tttagcggta cggagaattt tttccgcagg
    11641 caccataacg ccgagatcga caatttcgta gttgttacat tgcagcacca caccaacgat
    11701 atttttaccg atgtcgtgga cgtcgccctt cacggtggcg atcaccatct tgccgttggt
    11761 tttgccctgc tctttgctgg cttcaataaa cggttcgagg taggccactg cctgcttcat
    11821 cacacgcgcc gatttgacca cctgtggcag gaacattttc ccttcgccga acagatcgcc
    11881 gaccacgttc atgccgtcca ttagcgggcc ttcaatcact tcaatcgggc gcgtagcctg
    11941 ctggcgggct tcttcggtat cctgctcgat aaactcagta atgcctttga ccagcgagta
    12001 ttccagacgt ttattcactt cccacgagcg ccactccgcc tgctgggcgt tagcggtgtc
    12061 gtcggttttg ctgccgcgat atttctcggc aagctccagt aaacgctcag tgccatcatc
    12121 gcgacgatta agaatcacat cttccaccgc gtcgcgcagt tcagcgggca ggtcgtcgta
    12181 aatcgccagt tgcccggcgt tgacgatccc catatccatg ccattgcgaa tagcgtagta
    12241 gaggaacacc gcgtgaatgg cttcgcgcac cggatcgttg ccacggaatg agaaagaaac
    12301 gttagatacg ccgccggaaa tcagcgcgtg cggcagttcg cgtttgatgt cttcgcaggc
    12361 gccgataaag tcctgcgcat agttgttgtg ctcttcaatg ccggtggcga ccgcgaagat
    12421 gttcgggtcg aagatgatat cttctggcgg gaagccaacc tcttcggtga ggattttgta
    12481 cgcccgacgg caaatctcaa ttttccgtgc gcgggtatcg gcctgcccct gttcgtcaaa
    12541 ggccattacc accaccgctg caccgtaacg acgcaacagt ttcgcatgat ggataaaggc
    12601 gtcgacgccc tctttcattg agatagagtt aacaatgcct ttgccctgaa tacacttcag
    12661 ccccttttcg atgacctccc attttgagga gtcgatcata atcggcacgc gagcgatatc
    12721 cggttcaccg gcaatcagat tgagaaaacg caccatcgcc gcttcggcat cgagcatccc
    12781 ttcatccatg ttgatatcga taatctgcgc gccgttttcc acctgctgac gcgctacatc
    12841 cagcgcctcg ctgtatttct cttctttgat caggcgcttg aacttagcgg aaccggtgac
    12901 gttggtgcgt tcacccacgt tcacaaacag gctatcgtcg ccaatgttca gcggctccag
    12961 gccggacaaa cggcaggcta cgggaatttc aggcagtttg cgcggcgcta atccttctac
    13021 tgcacgactc atcgctgcaa tatgttgtgg cgtggtgcca cagcagccgc cgacgatatt
    13081 gagaaaaccc gcttgcgccc attcacgtat ctgttttgcc atcgtgtcgg cgtcgagatc
    13141 gtactcacca aaggcgttgg gtagcccggc gttcgggtgc gcggtgacgt agcattccgc
    13201 aatccgtgat agttcctgca cgtactggcg cagttcatct ggccccagcg cgcagttgag
    13261 gccaaaagtc agggcttcgg catggcgcag ggagttgtaa aatgcttcgg tggtctgtcc
    13321 ggagagcgtg cgcccggagg cgtcggtgat ggtgccggag atcataatcg gcagctcaac
    13381 gcctaacgct tcgaactccg ttttcaccgc aaagaccgcc gctttggcgt taagggtgtc
    13441 gaaaacggtt tcaatcagaa tcagatccgc accgccttcc actaatgctt tggtggattc
    13501 gcgataggcc gccaccagct ggtcaaaagt gatgttgcgg aatgccggat cgttcacgtc
    13561 cggagaaatg gacgccgtgc ggttggttgg gccgagaacg ccggcaacgt agcgcggttt
    13621 ctccggcgtg cgcgcggtcc actcgtcagc acaggcgcgc gccagtttcg ccgccgcaaa
    13681 gttgatttcc gccgacaggg attccatctg gtaatccgcc atcgcaatgg tcgtggagtt
    13741 gaaggtgttg gtttcgatga tatccgcgcc cgcttcaaag taggcgttgt ggatagcggc
    13801 gatcacttcc ggtttgctta gcaccagcag atcgttattg cctttgaggt cgcatggcca
    13861 gtcggcaaag cgttcaccac gaaaatcggc ttcgttcagt cgatagctct ggatcatggt
    13921 gcccataccg ccgtccagca ccagaatacg ttcatttaac tgcgcacgca gttgttccac
    13981 tttgctgctc acacttgctc ccgacacgct caacccagat ttaataaaaa ttcaacaaac
    14041 catactggca taaacgcatc tgtggtaaaa gcgaccacca cgcaacatga gatttgttca
    14101 acattaactc atcggatcag ttcagcaact attgcattag ctaacaataa aaatgaaaat
    14161 gatttccacg atacagaaaa aggagactgt catggtcgca cccattcccg cgaaacgcgg
    14221 cagaaaaccc gccgttgcca ccgcaccagc gactggacag gttcagtctt taacgcgtgg
    14281 cctgaaatta ctggagtgga ttgccgaatc caatggcagt gtggcactca cggagctggc
    14341 gcaacaagcc gggttaccca attccacgac ccaccgcctg ctaaccacga tgcaacagca
    14401 gggtttcgtg cgtcaggttg gcgaactggg acactgggca atcggcgcac atgcctttat
    14461 ggtcggcagc agctttctcc agagccgtaa tttgttagca attgttcacc ctatcctgcg
    14521 caatctaatg gaagagtctg gcgaaacggt caatatggcg gtgcttgatc aaagcgatca
    14581 cgaagcgatt attatcgacc aggtacagtg tacgcatctg atgcgaatgt ccgcgcctat
    14641 cggcggtaaa ttgccgatgc acgcttccgg tgcgggtaaa gcctttttag cccaactgag
    14701 cgaagaacag gtgacgaagc tgctgcaccg caaagggtta catgcctata cccacgcaac
    14761 gctggtgtct cctgtgcatt taaaagaaga tctcgcccaa acgcgcaaac ggggttattc
    14821 atttgacgat gaggaacatg cgctggggct acgttgcctt gcagcgtgta ttttcgatga
    14881 gcaccgtgaa ccgtttgccg caatttctat ttccggaccg atttcacgta ttaccgatga
    14941 ccgcgtgacc gagtttggcg cgatggtgat taaagcggcg aaggaagtga cgctggcgta
    15001 cggtggaatg cgctgacttt ttctggcggg cagaggcaat attctgcccg tcatacctga
    15061 gtggtaatag aataagggtg tctgttaatc gcattgacgt caaaataact tattgtcata
    15121 cacttcatac acttttatta actcagcatt atttttaaac atcaaaccac ttaattatta
    15181 acaaaatcgc aagatctcat tccaccgaaa cttttacggc tgcattatga gattgagcga
    15241 tttatgatat ttctttagtt gtaatcatat taaatttttt cactgttctg atagttaaaa
    15301 ttcaagacat caataaacaa tgagatattt aaatgattac tcatattcct cgtagttctt
    15361 tctc
//
LOCUS       DBFIFI010000096        15314 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.096, whole genome shotgun sequence.
ACCESSION   DBFIFI010000096 DBFIFI010000000
VERSION     DBFIFI010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.096"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            228..794
                     /gene="yceJ"
                     /locus_tag="N7B39_002870"
     CDS             228..794
                     /gene="yceJ"
                     /locus_tag="N7B39_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HGU2422824.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            798..1373
                     /gene="yceI"
                     /locus_tag="N7B39_002871"
     CDS             798..1373
                     /gene="yceI"
                     /locus_tag="N7B39_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HGU2422825.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1415..2467)
                     /gene="trhO"
                     /locus_tag="N7B39_002872"
     CDS             complement(1415..2467)
                     /gene="trhO"
                     /locus_tag="N7B39_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HGU2422826.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVKTFRAQLYAFDSALDGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2692..3612
                     /gene="lpxL"
                     /locus_tag="N7B39_002873"
     CDS             2692..3612
                     /gene="lpxL"
                     /locus_tag="N7B39_002873"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HGU2422827.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3784..5010
                     /gene="mdtG"
                     /locus_tag="N7B39_002874"
                     /pseudo
     CDS             3784..5010
                     /gene="mdtG"
                     /locus_tag="N7B39_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
     gene            5093..5467
                     /gene="msyB"
                     /locus_tag="N7B39_002875"
     CDS             5093..5467
                     /gene="msyB"
                     /locus_tag="N7B39_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HGU2422828.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(5468..5695)
                     /gene="yceK"
                     /locus_tag="N7B39_002876"
     CDS             complement(5468..5695)
                     /gene="yceK"
                     /locus_tag="N7B39_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HGU2422829.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(5868..8411)
                     /gene="mdoH"
                     /locus_tag="N7B39_002877"
     CDS             complement(5868..8411)
                     /gene="mdoH"
                     /locus_tag="N7B39_002877"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HGU2422830.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(8404..9939)
                     /gene="mdoG"
                     /locus_tag="N7B39_002878"
     CDS             complement(8404..9939)
                     /gene="mdoG"
                     /locus_tag="N7B39_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HGU2422831.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            10332..11489
                     /gene="mdoC"
                     /locus_tag="N7B39_002879"
     CDS             10332..11489
                     /gene="mdoC"
                     /locus_tag="N7B39_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HGU2422832.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGTFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(11497..12978)
                     /gene="clsC"
                     /locus_tag="N7B39_002880"
     CDS             complement(11497..12978)
                     /gene="clsC"
                     /locus_tag="N7B39_002880"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309451.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HGU2422833.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMSEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIVLKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(12920..13453)
                     /gene="ymdB"
                     /locus_tag="N7B39_002881"
     CDS             complement(12920..13453)
                     /gene="ymdB"
                     /locus_tag="N7B39_002881"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HGU2422834.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(13548..13859)
                     /gene="ymdA"
                     /locus_tag="N7B39_002882"
     CDS             complement(13548..13859)
                     /gene="ymdA"
                     /locus_tag="N7B39_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HGU2422835.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(13980..14312)
                     /gene="csgC"
                     /locus_tag="N7B39_002883"
     CDS             complement(13980..14312)
                     /gene="csgC"
                     /locus_tag="N7B39_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HGU2422836.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPDDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(14371..14829)
                     /gene="csgA"
                     /locus_tag="N7B39_002884"
     CDS             complement(14371..14829)
                     /gene="csgA"
                     /locus_tag="N7B39_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HGU2422837.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGGNHGGGGNNSGPNS
                     ELNIYQYGGGNSALALQADARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSA
                     TLDQWNGKDSHMTVKQFGGGNGAAVDQTASNSTVNVTQVGFGNNATAHQY"
     gene            complement(14870..15313)
                     /gene="csgB"
                     /locus_tag="N7B39_002885"
     CDS             complement(14870..15313)
                     /gene="csgB"
                     /locus_tag="N7B39_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HGU2422838.1"
                     /translation="MLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFNQAAI
                     IGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDASISQ
                     GAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT         3831 a         3916 c         3635 g         3932 t
ORIGIN      
        1 atctcccttt catttagcgt gaaaaattga atgagattat tcctgcttct acagcactca
       61 ccagacatct ctgatgctga acccactaaa cttatgaaga ttctttcatt attaattcaa
      121 aattattgaa ataaaaataa atatcattca atttgttatt tttgtgttgc aattcattct
      181 ccgacacgcc attttctttc tctcacaaat tcgttttgga tgcacttatg tcattcacaa
      241 atacccctga acgctatggt gttatatctg cggccttcca ttggctaagc gcaattattg
      301 tctacggcat gtttgcttta ggtctgtgga tggtcacgct cagttattac gatggctggt
      361 atcacaaagc acccgaacta cataaaagca tcggtatttt gttaatgatg gggctggtta
      421 ttcgcgttct gtggcgtgtc atatctccac cgccgggtcc gctgccgagt tattcgccca
      481 tgactcgtct tgccgcaaaa gctggacatc tcgcattgta cctattgctg ttcgccatcg
      541 gcatcagcgg ctatctgatc tcaactgccg atggtaaacc gatcagcgtt tttggctggt
      601 ttgacgtccc cgcgaccctt gccgacgctg gcgcacaggc agactttgcc ggtgccctgc
      661 atttttggct cgcgtggagc gtcgtagtac tgtccgttat gcacggattt atggccctga
      721 agcatcattt catcgataaa gacgacactc tgaagcgcat gctgggaaag tcgtcatctg
      781 actatggagt ataaaaaatg aaaaaaagcc tgcttggttt aaccttcgcg tccctgatgt
      841 tctctgccgg ttcagcggtt gccgccgatt acaaaattga caaagaaggt cagcacgcct
      901 ttgttaactt tcgcatccag catcttggct atagctggtt atatggcacc tttaaagatt
      961 tcgacggtac ttttaccttt gatgaaaaaa atccagctgc cgataaagtg aatgtgacaa
     1021 ttaacaccac cagcgtcgat actaatcacg ccgaacgtga taaacatctt cgcagtgcag
     1081 atttcctcaa taccgcaaaa tatccacagg caacattcac ctccaccagc gtgaagaaag
     1141 acggtgacga actggatatt accggcgatc tgacgctgaa tggtgtaacc aaacccgtca
     1201 cgctggaagc gaaattaatt ggtcagggcg acgacccatg gggtggtaaa cgtgcaggct
     1261 tcgaggccga aggcaaaatt aagctcaagg acttcaatat caagacagat ttaggtccag
     1321 cttctcagga agtggatctg attatttcag tggaaggcgt acagcagaag taagagtgaa
     1381 atgttgccgg atggcacgca tccggcaatg atatttattc tgttggatca ggaatgccca
     1441 gtgttgtatt cagacgtcca cgagacttat taaagatctt attgccattt tcacgtcctg
     1501 cccgacggcg tcgctgttct tctggcggta acgcgctttc ttcgcagcaa atttcactac
     1561 aacaaccttt gtatttttcc gcacatactg gacactgaat aaacagcagg tggcagccat
     1621 catttttaca gttggtatgg ctgtcgcacg gcgcaccgca ctggtggcaa tgcgcgataa
     1681 tctcatcaga tatacgttcg cccatccgct cgtcaaaaac aaaatttttg ccaataaaac
     1741 gcaccggcaa gccctgctcg cgcgccttac gggcgtattc aataattcca ccctcgatat
     1801 gccagacttt attgaatccg ttatgtttca tccaggcact ggccttttca caacgaatgc
     1861 cgccggtgca gtacatgacg atttttttat ctttatgtgc ctgcatcatc tcgactgctt
     1921 ttggcagctg ctcacggaag gtatctgccg gaatttccag cgcgttttca aagtgcccca
     1981 cttcatactc atagtggtta cgcatgtcga taaacagtgc atcgggatcg tcaagcatgg
     2041 cgttcacttc cgccgcttgc agatactcac caacattgct ggcatcaaag tgaggatcgt
     2101 caataccgtc ggcaacgata cgatcgcgga ccttcatgcg cagtacccag aaggatttcc
     2161 cgtcatcatc caacgcgata ttcaggcgta agccatctaa agccgagtcg aaggcataga
     2221 gctgcgcgcg aaatgtttta acattgctcg caggtacgct gatttgcgcg ttaatgccct
     2281 catgcgccag atacactcgc ccaaaaacat tcagcgcggt aaacagctga tataaagcgt
     2341 cacgggtcgc cttaggatcg gcgatgtgga aatacttgta aaacgaaatg gtggttcgcg
     2401 gttcgctctc agccaacatt ttggctttta acgcgtcgtt ggaaatgcgg ttgtgtaaca
     2461 ctggcatggt gtacggttcc tgcgagatgg gaaagtaaaa atccgcggca tgatatagca
     2521 attatcgata attaacatcc acacatttta cgctacattt gcgcattaaa aattatttgt
     2581 tatttacaag cgcggcaatt tcgcccagtc ttcagccaca aattttggtt gcgggcgaaa
     2641 aaatgcgaca atacatacaa ttgcccgaat aggttgaaaa acaggattga tatgacgaat
     2701 ctacccaagt tctccaccgc actgcttcat ccgcgttatt ggttaacctg gttgggtatt
     2761 ggcgtacttt ggttagtcgt gcaattgccc tacccggtta tctaccgcct cggttgtgga
     2821 ttaggaaaac tggcgttacg ttttatgaaa cgacgcgcaa aaattgtgca tcgcaacctg
     2881 gaactgtgct tcccggaaat gagcgaacaa gaacgccgta aaatggtggt gaagaatttc
     2941 gaatccgttg gcatgggact gatggaaact ggcatggcgt ggttctggcc ggaccgccga
     3001 atcgctcgct ggacggaagt gatcggcatg gaacacattc gtgacgtgca ggcgcaaaaa
     3061 cgcggcatcc tgttagttgg catccatttt ctgacactgg agctgggtgc gcggcagttt
     3121 ggtatgcagg aaccggggat tggcgtttac cgcccgaacg ataatccact gattgactgg
     3181 ctacaaacct ggggccgttt gcgctcaaat aaatcgatgc tcgaccgcaa agatttaaaa
     3241 ggcatgatta aagccctgaa aaaaggcgaa gtggtctggt acgcaccgga tcatgattac
     3301 ggcccgcgct caagcgtttt cgtcccttta tttgccgttg agcaggctgc gaccacgacc
     3361 ggaacctgga tgctggcacg gatgtccggc gcatgtctgg tgcccttcgt tccacgccgt
     3421 aagccagatg gcaaagggta tcaattgatt atgctgccgc cagagtgttc tccgccgctg
     3481 gatgatgccg aaactaccgc cgcgtggatg aacaaagtgg tcgaaaaatg catcatgatg
     3541 gcaccagagc agtatatgtg gttacaccgt cgctttaaaa cacgcccgga aggcgttcct
     3601 tcacgctatt aaatctccca tgccggatgc ttcagaatgg catccggcat taccacagca
     3661 aatccccctg atttagcgat aaaagctctc tggattgcgc cccctggaag tcgggcgcat
     3721 aattagtgtg cttatctttt cttcttatgt tcaccgcgcc tggcgcacca acagcggatt
     3781 gctatgtcac cctgtgaaaa tgacacccct ataaactgga aacgaaacct gatcgtcgcc
     3841 tggctaggct gttttcttac cggtgccgcc ttcagtctgg taatgccctt ctaacccctc
     3901 tacgttgagc agcttggcgt taccggccac tccgccctga atatgtggtc cggtattgtc
     3961 ttcagcatta catttttatt ttcggccatc gcctcaccgt tttggggtgg actcgccgac
     4021 cgtaaaggcc gaaaactcat gctattacgc tctgctctcg gcatgggcat cgtgatggtg
     4081 ttgatggggc tggcacaaaa tatctggcag tttttgatcc tgcgggcgct tcttgggtta
     4141 cttggcggat ttgtccccaa cgctaatgct cttatcgcca cacaagtacc gcgtaataaa
     4201 agcggctggg cactgggtac gctctccaca ggcggcgtta gcggtgcgtt gctcggccca
     4261 atggctggcg gcctgctcgc cgatagctac ggcttacgtc cggtattctt tattaccgcc
     4321 agtgtgctca tactctgctt tttcgtcacc ctgttttgca tcagagaaaa attccagccg
     4381 gtcagcaaaa aagagatgct gcacatgcgg gaagtggtga catcacttaa aaacccgaaa
     4441 ctggtactca gcctgtttgt caccacgtta atcatccagg tggcgacggg ctcaattgcc
     4501 cccattctga cgctgtatgt ccgcgaactg gcgggtaacg tcagtaacgt cgcctttatc
     4561 agtggcatga tcgcctcggt gccaggcgta gctgctctgc tgagtgcacc acgactcggc
     4621 aaacttggcg atcgaatcgg acccgaaaag atcctgatta cggcgctgat cttttctgta
     4681 ctgctgttga tcccaatgtc ttacgttcag acgccattgc aacttgggat tttacgtttt
     4741 ttgctcggtg ccgccgatgg tgcactactc cccgccgtac agacactgtt ggtttacaac
     4801 tcgagcaacc aaatcgccgg acgtatcttc agctataacc aatcgtttcg tgatattggc
     4861 aacgttaccg gaccattgat gggagcagcg atttcagcga actacggttt cagagcggta
     4921 tttctcgtca ccgctggcgt agtgttattc aacgcagtct attcatggaa cagtctacgt
     4981 cgtcgtcgaa taccccaggt atcgaactga tttttcgcct ttcatacttg caaaagcgga
     5041 gaatcagcta tccttttccc tgaaacctca tcaactcaaa gggagaatcg tgatgaccat
     5101 gtacgcaacg cttgaagaag ccattgacgc tgcacgcgaa gaatttcttg cagacaaccc
     5161 cggcatcgac gccgaagatg cgaatgtgca acagttcaat gcccaaaaat acgttttgca
     5221 ggacggcgac atcatgtggc aagttgagtt ttttgccgac gaaggggaag aaggtgaatg
     5281 tttacctatg cttagcggtg aagccgcgca aagtgttttt gatggcgact atgatgagat
     5341 agagatccgc caggagtggc aggaagagaa tacattacat gaatgggacg agggggaatt
     5401 tcagcttgag ccaccgctgg ataccgagga aggacgcgca gcagctgatg agtgggatga
     5461 acgttaatta ctcatacggg ccatgatgaa tgtcgatcgg cagcagtaaa gtatcaaaaa
     5521 ccagggagaa tggcagatca aggattgtga cataacgcca ggcggagtca cgcacatccc
     5581 attgcacacc gggataatat tggttgccat gcccctgccc cggtatagtg cggctaatga
     5641 tgctgccaca gccactcagc aaggtcacca tgatgctcac cacaattaaa cgcatttgat
     5701 acctcttccg taaaaaaata ccggcataac gccggtattt ttattggctc gttgtttttg
     5761 ccagatgtgg cgtgaacgcc ttatccgacc tacaaaatct tgcaaaatca acacattgca
     5821 ggagcgatgt aggcctgata agcatagcgc atcaggcaac tacgttttta ttgcgaagcc
     5881 gcatccggtt tacgcaatgc cagtggattg agctttatcc cttcgtaata actcacccat
     5941 gaagaatatc tctcagggga attccagacg cggaaatgca gacgggccat cgtcaccgga
     6001 tcgcttagca gcacaaggcg acgatcgcga ttcagcttct ctggcgtctc gttcagcgcc
     6061 tgttcaacat ggcggtcacg ggcgatttcc agcaccttac tggcgcggtg acgcgcggtc
     6121 gccattgcgg ttgccagagc gttaaatgac gggttaaaca ctgcgtgcat aaaaccatca
     6181 tcaagggagc gttgacggtt catctcaagg aaccgatcgg tatcaaccag tacctgcggc
     6241 ggtgaatact cttccgggat caggaacagt ttccagcgtt tggtgcgcag accaacggtg
     6301 gcacggctgg aaatcaccga aacaaacggt gacaggatca acgagaagac aatcggtgcc
     6361 agccagaaca ggaaacgcag atccaaccac gccatcccga cagcccacac taaccctaac
     6421 agcagctgtg agccgtggcg tttgaacgct tcaccccagg aagtggagtc atcatcacgc
     6481 tgcggtgaat tccacaccac ttcccagcca aggaacgcgc tgacgacgaa gaccgtatgg
     6541 aacagcatgc gtaccggagc cagcagcacg gaaaacagca cttccagcag caacgataat
     6601 gtaacgcgcc agaagccgcc atattctttc gttcctttac accaaataag caaaatgctc
     6661 aatagctttg gcaggaacaa cagcaccatg gtcgaagcaa aaagtgcaat cgccagctca
     6721 ggacgccact gcggccacac cgggaacaac tgccgtggtt gcaggaagta ttgcggttcg
     6781 gtcaacgcat gcactacctg caatgcagta gagagcgcga ggaacataaa ccacagcgga
     6841 gcggagagat aagacatcac gcccgtcagg aataccgcac ggtgaaccgg gtgcataccc
     6901 ttcaccagga acagacggaa gttcatcagg ttaccgtggc accagcggcg gtcacgtttt
     6961 agctcatcaa gcaagttagg cggtaattct tcataagaac ccgggagatc gtaagcaatc
     7021 cagactcccc aacctgcacg gcgcatcaac gccgcttcca cgaagtcatg tgacaggatt
     7081 gaaccggcaa aggaaccttc gcccggcagc ggggccagtg cacagtgctc gataaacggt
     7141 ttcacgcgga taatcgcgtt atgcccccag tagtgcgact cgccaagttg ccagaagtgc
     7201 aaaccggctg taaacagtgg cccatacacg cgggtcgcga actgctgaca gcgtgcatac
     7261 agcgtatcca taccggacgc tttcggcgac gactgaatga tcccggcgtt cgggttggct
     7321 tccatcaggc gcaccaggcc gcacaaacaa tcaccggtca ttaccgagtc agcatccagc
     7381 accaccatat agctgtactg gctgccccag cgacggcaga agtcatcgat attaccgctt
     7441 ttacgcttca cgcgacggcg gcggcggcga tagaaaatct gaccttcgcc accgacttca
     7501 gcgataagct ccatccaggc tttttgctct gcgacgcaga tatccgggtt gtaactgtca
     7561 ctcagaatgt agacatcaaa gtgtttggca ttcccggtgg cttttactga ttcccacgtt
     7621 gcacgcaagc cagcaaaaac acggttcacg tcttcgttac agataggcat gatcaacgcc
     7681 gtgcgatgct ccgggtttaa tggttcatcg ccaactgttg acgcagatat actgtattta
     7741 tcgcgaccaa taagtagttg caggaagccc attaacgccg tccagaatcc ggcggacacc
     7801 caacagaaca gtaccgcaaa gaggatcagg ataccggttt gcagcatata aggcagaagc
     7861 tgcataaagg aaacccacaa atcctgacca accatatcca taggattaat cagcgcccaa
     7921 ccctgataag gaagaatggt cttcatatac caggtcgcga cgacagtttg cgcgagcgtc
     7981 aggatcaaca gaatgtaacg gcggatggta ccgacggtac gccacttttg ctcgctctcc
     8041 tgctcttctt tggtcaaacg agccagatag cgcggtgtta catcgcgtcc acgcaggcga
     8101 tcccagaaac ggcctaccgg gttggtacgc cacgggtcgg gaaacatcga ggagcgtttt
     8161 gcttctggca tcgccttaag ctgatcgcgc ccttcgtcat ctttaattaa ctgtccatca
     8221 gcaagtgaat ctggccaggc ttgttccaga cgcgccttta ccgagccttg cggggagtca
     8281 tcctcccgcg cccaggtgcg gtgttcggca tccagcgcct gatgaacggc gcggatatca
     8341 gtcttcggca atgccgcttt ctcgcttgcg gcgatgggca ttgcgtcaat gtactcagtt
     8401 gtcttattca ttggcaggta actggtagct ccaggtttca ctcaacgtct gatcggcatt
     8461 caccagcgca gcgcgcattt cagtggtttt cttggcatct ttcactttca cacgcatcac
     8521 cagacgccag cctttggtga ccgggttata gcgcaccgtg ctttcaacta tctcaccatt
     8581 atcaccaatg ctggtttgcg ctgtgaccgg ggtatcctct ggcagttttt tcatctctgc
     8641 gccggtaaaa tcgaccacaa aggcgatagt accgtcaggc tggcgaatca ggttcgactg
     8701 cttcacatcc cccgttgaac gacgcgtttg ttgcacccat gcgttatctg gcgcatgcag
     8761 tttgtcttca tcacggctga aggtgatggt gtatttaaag ttcatctctt tacccggctc
     8821 cggcagctga tccggcgtcc agtaagcgac gatgttatcg ttagtttcat cgttggttgg
     8881 aatttccacc agctcaacgc tgcctttacc ccactcccct ttcggagtca cccatgcgct
     8941 tggacggaga tcgtaacgat catcgagatc ttcaaagcgg gagaaatcac gaccgcgctg
     9001 caacagacca aagccttgcg ggttttccat ggagaagctg ctgaccgcta aatgtttcgg
     9061 gttattcaac ggacgccaga tccattcgcc attaccggca tggatagaga gaccgttaga
     9121 gtcgtgcaac tccggacgat agttatttgc tggcgacggt tggttcggcc caaacaggaa
     9181 catactggtt aacggtgcaa cccccagttt gccgacttta tcgcgcagat agattttcga
     9241 ctgcacatcc acaaccgtgt cacgccctgg cataactacg aatttgtaag cacctgtcgc
     9301 gcgcggcgag tcaagcaatg cataaatggt taaacgttta tcagttggtt ttggacgctc
     9361 gatccagaac tctttgaagc gtggaaattc ttcacccgat ggcaaggcgg tatcaattgc
     9421 cagaccgcgg gcagaaaggc cataaacctg acctgcacca atcacgcgga aatagctggc
     9481 cccgagcatg ctgacgattt catcgttttt atctttgctg ttgatcgggt aaagcacttt
     9541 aaaaccggca aaaccaaggt cttttaccgt gtctttgtca tgctgaacat cgccgaaagt
     9601 gaaataatcc gggctgtatt tgattcgttt gactgcggtg gcagtcactt catttatttt
     9661 gaccggggta tcgaagtaca taccctgatg gtagaactcg agtttgaatg gggtcttcag
     9721 attgttccag tacgctttgt catgattaaa ctggatctgc tgatagtccg cgtatttcat
     9781 atcgcggaaa acggagggca agttgctttt gggcgcctca tagcctttcc cggctaagga
     9841 ttgagcttgc tttgcgacat catcaatact gaaagcccag cttgaagatg tatacagggt
     9901 taacattact gcagcactca accaacgcat tttcatcatt tgtagtttat gtttcataat
     9961 tagtaagcac ttcccccttt gtgtgcttat atcgatccga tctattttaa tggataacca
    10021 gccattccga caacagaatc ccggtatcgt tcagcacgct ggctcatgga ttaagcacct
    10081 gattgcaacc gttttcactt tccgtgcttc tcctaaagac agaagcatcg acttcgtgta
    10141 gggtttgcca gcaaatttat ttatcaaccg tcttacagac gcttccattc tggttagtgg
    10201 tagcgatgat gctatgcata aaaacttgaa gcctgcgcct ggcgcggtca ggtcggtgtc
    10261 gctatggcaa caagcctaaa ggcataaccc gacagggata agacgaaaaa tccgagatta
    10321 gttaaccata tatgaatcca gtacccgcgc aacgtgaata tttcctcgac tccatccgcg
    10381 cctggctgat gttgttaggg atcccttttc atatttcttt aatctattcg agccatacat
    10441 ggcatgtgaa tagcgccgaa ccgtcattgt ggctgaccct ttttaatgac ttcatccact
    10501 cgttccgcat gcaggtattt ttcgttatat ccgggtactt ttcctacatg ctttttttac
    10561 gctatccctt gaaaaaatgg tggaaagtac gtgtcgaacg tgtagggatc ccgatgttaa
    10621 cagccatccc cctactgaca ttgccgcaat ttattatgct gcaatacgtc aaagggaaag
    10681 cggaaagttg gcctgggctg tcattgtatg acaaatataa tacgttggcc tgggaattaa
    10741 tatcacatct gtggttttta ctggtgttag tggtcatgac gacgctgtgc gtatggatat
    10801 ttaaacgcat cagaaataat ttagaaaatt ctgataaaac gaataaaaaa ttctcgatgg
    10861 taaaactatc ggtgattttt ttgtgcctcg gcatcggtta tgcggtaata agaagaacga
    10921 tttttattgt gtatccaccc attctgagta atggcacgtt caattttatt gtcatgcaaa
    10981 cgctatttta tttaccgttc tttatcctcg gcgcactggc tttcattttc cctcatctta
    11041 aagccttgtt taccacgccg tctcgtggct gtaccctcgc tgcagcattg gcgtttgtcg
    11101 cttatttact caaccagcgc tatggcagtg gcgatgcctg gatgtacgaa accgagtcgg
    11161 tgatcaccat ggtcctcggt ctgtggatgg tgaatgtggt cttctccttt ggccaccgtt
    11221 tgcttaactt ccagtcagcg cgggtgactt attttgttaa cgcatcgctg tttatctatc
    11281 tggttcacca cccgttaacg ctgtttttcg gcgcatacat tacaccgcac atcacctcca
    11341 actggcttgg ttttctctgt ggcctgatat tcgtagtagg gattgcgata attctgtatg
    11401 aaattcattt gcgcatcccg ttactgaagt ttttgttctc tggtaaaccg gttgttaagc
    11461 gtgagaacga taaagcacca gcccgttaag ccacatttac aataaccatt ccacgggcaa
    11521 tatcgacgcc agtctgacca taacccgctt ccagaaactg gtagcgggtt cttttttgag
    11581 aacaatctct ttctttgcat gacgatcaac ccagttgatc cgtccccacc tgtccagacg
    11641 gagctgccag gccgcatcat actggctctg aataaagcgt ttatcaatta actgagccag
    11701 cgtttcgctc tctatcacga agcccatttc agtattgagc aatgttgaac gcggatcgaa
    11761 attgaaagaa ccgataaaca ccgttttacc atcgatgcta aaggttttag catgcaggct
    11821 ggcaccggaa ttaccggtta tgccgcgatc gtgtaacgta ctactttgtt cacgcgtcgg
    11881 cttgagttca tataattcca cgccatagcg gagcaatttt ttgcgccagc gcgcgtatcc
    11941 ggcatggacg acagcaacat cgttagcggc aagagaattg gttaggatcg caatctttac
    12001 cccttttctc accatccgta agagttgcgc cacacctgcg cgtgtcggta caaaatagga
    12061 agagataata tcgatgcgtt cactgggtga gcccatgata tcgaacaggc gctgcggtaa
    12121 cagtgaatgc cgttttgcct tgccctcccc tttcgccgga tcatcactta ataaacgtgt
    12181 tttcgcccag ataagcggca acgttccatc aaccagatga tttataaacg gactggattc
    12241 cattttgcgt aagtaacgat gcgtcatggc atcgttatgc caggaggcgg gtaactcgat
    12301 gcgatccgcc atttcacctt ccgggacatc cagcacctgc tgtaagggtg aaaccgattt
    12361 gcaataccag tagcgggcga aatcatcggc aacgtcctct actacgggtc ctattgccat
    12421 gacatctaaa tccgaaaaaa gtggctcttc ccctgcccca aaataggcat caccaatatt
    12481 tcgcccccct accaacgtca ccacgccatc gacagtgaaa cttttattgt gcatacggcg
    12541 attgagacgg gaaaagtcgg tgatataacc aagcggacgc agcaagcgaa acgagaaagg
    12601 attaaaaagc cggacttcaa tgcggggatg gctgtcaagc aagcgtaaaa tgtcgtcaag
    12661 tccgggcgta ttgttgtcgt ccagcaacaa acggacgcga acgccacgct ttgctgcggc
    12721 taacagggcg gaaaacagta accgacccga catatcgtcc tgccagatgt aatactgaac
    12781 atccagcgta tgctctgaca tttcggccag acgataccgg gcggcaaacg catccagact
    12841 tttctccaga gggaaaagac cacactgacc gggatgttgc gaacacagtg gcagcaccgc
    12901 gctcgccagc cggggcaaat cattcatctc cttgttgggt aaggagtctt tcgtagaggt
    12961 gggcgttttc ttcatcataa cagacaaagt atacctgttc gggtaaagcg tgacgggtaa
    13021 taaattctga aacggttttt actgcgattt cagccgctgc cgcacgaggg taaccataaa
    13081 ccccagtact gattgcagga aaagccactg acgtatagct gtttgccgcc accagtcgta
    13141 ggctattgag ataggcatcc tgcaaaagct ggtcttcgtt ttgttcacca ccacgccaga
    13201 ctggcccgac ggtgtgcact acggctttag caggaagatc gcctgcaagc gtaataacgg
    13261 catgccccgt agggcaatcg ccctgctgtt gcctgacttt taaacaagca tccagcaggg
    13321 ccggacccgc tgcgcgatga atggccccat cgacgccgcc acctcccatt aatgaaggat
    13381 tagccgcatt cacaatcaca tcaacggcca gtttggtaat atcaccctgc acaacatgaa
    13441 tacgcgtttt cataacgcct ccttacacct tcttgtttct taagtgtaca gcaggctttt
    13501 tggttaaatc gcatttttct gctcagcaac gtgagttgtt cagaaactta gttatattcg
    13561 atgttcatta cagccagctt tttctgttta tcaagatact gcacttttac tgaggctatt
    13621 tgcgcgtttt tgacattgcc gctcattaac gctttgctgg agtaggtttt acccggaaca
    13681 gaaccattct gtgggcagtt catttctacg ggtgatgact gggtgctgac gtcacatgcc
    13741 ggttccacaa tttggccata aaaatggatc acgccccctt gcgcggtgct tcctgcgatg
    13801 gcaataaaag gaaaaaacaa actgccgagc ataagagagt ttaaaaaagg tctgaacatt
    13861 tattctattt cctcaatgat tagtcatcct tgagggttgt gttatccata ctttcaggtt
    13921 ggctgattcg cagcagacca ttctctccag attcatctta tgctcgatat ttcaacaaat
    13981 taagactttt ctgaagaggg cggccattgt tgtgataaat gaagtgactg tccatcagaa
    14041 acagtaacaa ctattttcac ccgatcgtcc ggggaaatat ttaaactcaa cttcgtcaaa
    14101 gcaatgggtt gattagcagg caatgaaagg gtcttttctt gcttcgtctg actttgccct
    14161 gaactgcctt cgcgcaggga caatatttgt actctgcaca gacaagattg agtaagagtg
    14221 acttcaggaa taatggtgta catatcccct tgctgggtcg tattaaaggt tatctgactg
    14281 gaaagtgccg caaggagtaa taacgcattc atattcttct cccgaaaaaa aacagggctt
    14341 gcgccctgtt tctttaatac agatgatgta ttagtactga tgagcggtcg cgttgttacc
    14401 aaagccaacc tgagttacgt tgacggtgga attagatgca gtctggtcaa ccgctgcacc
    14461 gttgccgcca ccgaattgtt taactgtcat atgagagtct ttaccgttcc actgatcaag
    14521 agtggcgctg ttaccaaagc cacgttgggt cagatcgatt gagctgtcat ctgagccctg
    14581 accaacatct gcaccgttac caccaccatg ctgggtaata gtaagatcag agttacgagc
    14641 atcagcttgc agagcaagtg cagagttacc accaccgtac tgataaatat tcagctctga
    14701 attcgggccg ctgttattac cgccaccacc gtggttaccg ccaccgccgc cgtactgagg
    14761 aacaacacct gccagagcgc taccggagaa tacgattgct gcaattgctg ctacttttaa
    14821 aagtttcatg taaaaccccc atcggattga tttaaaagtc gaatggaaat taacgttgtg
    14881 tcacgcgaat agccatttgc gactgtctct gcactacaat tgccgttttt tgagtaccat
    14941 actgtgtaat atttgcttta ttaccagaac ctttctggat aatcatcgca gtattaccat
    15001 aagcaccttg cgaaatactg gcatcattgg cactgcccgc ctgatcaata tatgcaaggt
    15061 tataatctcc tgtctggtca atctttgccc ggttgctact accttcttgc gcaacaaccg
    15121 ccaaaagttt tgagcctccc tgccgtaact gagcactatt attagtccca gcttgaccaa
    15181 ttatggctgc ctgattaaat gaagacttac tcaattcatt taccgcgaag ttatattctg
    15241 aattagctaa atcataacct gctgcggctg caatcccagg cgcacccagt attgttaaca
    15301 tcataaataa caat
//
LOCUS       DBFIFI010000097        15099 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.097, whole genome shotgun sequence.
ACCESSION   DBFIFI010000097 DBFIFI010000000
VERSION     DBFIFI010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15099
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.097"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            286..942
                     /gene="pphA"
                     /locus_tag="N7B39_002886"
     CDS             286..942
                     /gene="pphA"
                     /locus_tag="N7B39_002886"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HGU2422839.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLSERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTFVQLQ
                     "
     gene            complement(943..1134)
                     /gene="yebW"
                     /locus_tag="N7B39_002887"
     CDS             complement(943..1134)
                     /gene="yebW"
                     /locus_tag="N7B39_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HGU2422840.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(1239..1475)
                     /gene="yebV"
                     /locus_tag="N7B39_002888"
     CDS             complement(1239..1475)
                     /gene="yebV"
                     /locus_tag="N7B39_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HGU2422841.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(1593..3032)
                     /gene="rsmF"
                     /locus_tag="N7B39_002889"
     CDS             complement(1593..3032)
                     /gene="rsmF"
                     /locus_tag="N7B39_002889"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HGU2422842.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKTSVADFLRLTAPYGWTLTPIPWCEEGFWIERDDEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAALPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRSGGTLVYSTCTLNREENESVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPTPKYKVGNFPFSPVKD
                     REAAQIRQAAASVGLNWDGNLRLWQRDKEVWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNENAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTSNA"
     gene            complement(3113..5746)
                     /gene="yebT"
                     /locus_tag="N7B39_002890"
     CDS             complement(3113..5746)
                     /gene="yebT"
                     /locus_tag="N7B39_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HGU2422843.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTNNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEACSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(5715..6998)
                     /gene="yebS"
                     /locus_tag="N7B39_002891"
     CDS             complement(5715..6998)
                     /gene="yebS"
                     /locus_tag="N7B39_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HGU2422844.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVTSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            7128..7625
                     /gene="msrC"
                     /locus_tag="N7B39_002892"
     CDS             7128..7625
                     /gene="msrC"
                     /locus_tag="N7B39_002892"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HGU2422845.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            7722..8420
                     /gene="proQ"
                     /locus_tag="N7B39_002893"
     CDS             7722..8420
                     /gene="proQ"
                     /locus_tag="N7B39_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HGU2422846.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTAKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            8440..10488
                     /gene="prc"
                     /locus_tag="N7B39_002894"
     CDS             8440..10488
                     /gene="prc"
                     /locus_tag="N7B39_002894"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HGU2422847.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            10680..11561
                     /gene="htpX"
                     /locus_tag="N7B39_002895"
     CDS             10680..11561
                     /gene="htpX"
                     /locus_tag="N7B39_002895"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HGU2422848.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(11607..12980)
                     /gene="yebQ"
                     /locus_tag="N7B39_002896"
     CDS             complement(11607..12980)
                     /gene="yebQ"
                     /locus_tag="N7B39_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2422849.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQILTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACISGLRI
                     TQPRSRA"
     gene            13157..13948
                     /gene="kdgR"
                     /locus_tag="N7B39_002897"
     CDS             13157..13948
                     /gene="kdgR"
                     /locus_tag="N7B39_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HGU2422850.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(14092..14331)
                     /gene="yobH"
                     /locus_tag="N7B39_002898"
     CDS             complement(14092..14331)
                     /gene="yobH"
                     /locus_tag="N7B39_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HGU2422851.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAELGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            14491..14634
                     /gene="mgrB"
                     /locus_tag="N7B39_002899"
     CDS             14491..14634
                     /gene="mgrB"
                     /locus_tag="N7B39_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HGU2422852.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            14709..14996
                     /gene="yebO"
                     /locus_tag="N7B39_002900"
     CDS             14709..14996
                     /gene="yebO"
                     /locus_tag="N7B39_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HGU2422853.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVEDEDIIRLVAER"
BASE COUNT         3981 a         3652 c         3856 g         3610 t
ORIGIN      
        1 aactcgaaaa aaatggtaac taattgaatg tattaatatg taatagtacg tgttagagat
       61 taaaagatga gcataaattt atttaacaca ttaattctaa aagattttgt tgtttgttga
      121 cgaaaacagg aatcgtgttc ggtctctttt tatctgttaa aagccagaag catttccttc
      181 gctgacttta tagtcaacca taacacacac tctactgtct gagtccagcg ttttttaaca
      241 ttcttgttaa gattatgtga tctttagcgc gggaggaaaa tattgatgaa acagcctgcg
      301 cccgtttatc agagaattgc gggtcatcaa tggcgacata tctggctttc tggcgatata
      361 cacggttgtc ttgagcagtt gcgccgcaaa ttatggcatt gtcgttttga tccgtggcga
      421 gatttactta tctcagtggg agacgttatc gatcgtgggc cgcaaagttt acgttgtctg
      481 cagttactgg aacaacattg ggttcgtgcg gtaagaggca atcatgaaca gatggcgatg
      541 gatgcgctgg catcccagca gatgtctttg tggttgatga atggcggcga ctggtttatt
      601 gcgctggcag ataatcaaca gaaacaagcg aaaacggcgc tggaaaaatg tcagcatttg
      661 ccctttattc ttgaagtaca cagccgcacc ggcaaacatg ttattgctca tgccgattat
      721 ccagatgatg tttatgaatg gcaaaaggac gttgatttgc atcaggtctt gtggagccgc
      781 tcgcgattaa gtgaacgcca aaaagggcag ggaattacag gtgctgatca tttctggttt
      841 ggtcatacac cgttgcgcca tcgcgtggat attggcaacc tgcattatat tgataccggc
      901 gctgtctttg ggggcgaact gactttcgtg caattgcaat aattaaaaat caccatactc
      961 ctgtgctggt cgccagaaac catctataaa atcctcaatc ggaaaacaac cgccatgacg
     1021 gatccgttga tcgctcatag aataaagaca ctgctgttcc gtgttgtaga catccacaac
     1081 aatatcttca caaccgccat ccaggtagca aacaaaaagt accagcgcga acatttcatc
     1141 cccgaagtgt ggtgccgtac cgttaagttt aggagagatt ttacaacggg ggaataacca
     1201 ggacaaataa cccgccatgc cgacgggttc tttttggatc aggcaagacg cataatccag
     1261 gcatcagatt gttgatcgta acgggtacgg taaaggacag agtgttcgcc atttagcagg
     1321 gcagggatac tggtttcagc atcccaggca tccagttgca tacataaatc ggggtctgat
     1381 ttacaaggaa tggttaacgt tcgatcaccc ggcgattcac cgtgtaattc gccgtcgtcg
     1441 atttcaaaag cgcctatgcg cacactggtt ttcatcttcg tgctctccat ttagctgccg
     1501 gttgtggtac gaccagtccg atcgcaccat ttttcagcct agttgaggcc gccgaatgtg
     1561 ccagtcaaaa agtgcgcttt ttttgtgcgc tgtcatgcgt tactggtaaa aagtttcccg
     1621 tcccgcacca gttcacgcgg gtagctgttt ttcagtcgcg aaccaatccg tttggctaaa
     1681 ccaatcggct gatgctggaa agtaaccaac acgtcatccg ccactggcgc ggcttgcggg
     1741 taaacatcgc gcccgcgata ccactcctcc gcttcttgcg gtgtcagttc aaaagcgttc
     1801 tcgttgtcgg gggaggcaag ggcaataacc gcttcatgct gccagcgata acctttgttg
     1861 tgcgtttcgg caagtttaat ccccaggcga gaaaatcgga ctttaccgat cagggcttca
     1921 atgcctaccg gaaacaacca cacttctttg tcgcgctgcc agagtcgcag gtttccatcc
     1981 cagtttaagc caacacttgc agccgcctga cgaatttgtg cggcttcgcg atctttcacc
     2041 gggctgaacg ggaaattgcc cactttgtat tttggggtgg gtaaggcggg aatggcctga
     2101 gttttacgca gacgagcaac gaagaagcct tcgcagtcgt aaatttgtgg gaaaacatgc
     2161 aaaaagcctt cttcggtcag cgctttgttt gcaccaggga agagatcgcc aagtggtaaa
     2221 aactctactg cgtcggggta agtctcttta agccacaggc aaacggattc gttttcttcc
     2281 cggtttaagg tacaggtcga gtaaaccagt gtaccgccag agcgtaatgc atgaaaggca
     2341 ctgtcgatca gttcccgttg ggtagctgcg atttcctgat tgctttctgg tgaccaattt
     2401 tttagcgcat cgggatcttt acgcaccacg ccttcgccgg agcagggagc gtccagcaaa
     2461 atggcatcga acatttctgg caatgccgca ccaaacacgc ggccatcaaa atgtgtgagc
     2521 gcaacattac tgataccaca gcggctgata ttggcatgta acacttttac ccgactggcg
     2581 gaaaactcat tggcaaggat tgccccttcg ttattcatcc gcgcggcaat ttgcgtcgtt
     2641 ttggagcctg gcgcagcagc gacatccatc acccgctgtg gtgcattacc gtcagcaaac
     2701 aaggcggcga cgggcaacat tgaactggct tcctgaatat aaaacaggcc acttaaatgc
     2761 tcggcggtac tacccaatgg caatgcatct tcatcgtcgc gttcaatcca gaaaccttct
     2821 tcacaccacg gaattggcgt aagcgtccag ccataaggag cggttaatcg caggaaatca
     2881 gcaacggagg ttttcagcgt attaacgcga atgctgcggc gcaacgggcg ctgacaggcg
     2941 gcaagaaaat catcaaaaga gagcgtcgaa ggcattgctt cgcgcatttg cgtcagaaag
     3001 gcgtccggga aataaacggt gtgttgggcc acgagcatat accactggca aaaaaaacag
     3061 gcgcgcagtt tagcataaac gctccggcgc aggcacgccg gagcagtggg cattatttgg
     3121 gaagcgcagt tccccattca cgccactctt tcggttcact ttcctgcaac aggaagtgct
     3181 tgccttcctg ggctttcggt gccagtggcg tacccggagg cgtggcgaag gcgatgccgc
     3241 cacggataaa ctggttaaag gtgccggttt tcactacgcc gcccgtcaga ccaaagtcca
     3301 gactgtaacc cgatgccaac cagaagacgg aattgttacg caccaggtgt tgatagcgtt
     3361 tactgatgcg catcgcaatc atcacgcgat ctgacaatgt ccccagcgtc attccggtaa
     3421 ccgtaccgac ttccagacca cggaacagca caggcgtacc gatgcctaac gaacaggctt
     3481 ccggcgcttc aacaataatg cttaaaccat ccaggtaacg cgaatcagta atggtggcct
     3541 cttgtaattc aaagtcgcga cgaggattgc cacggcctgg ttcgacgttg atatacggct
     3601 ggaggatagt atcaagatgc tcaacgccag ctgccgaaat ttgcggtgtg accactgaga
     3661 agcgcgtacc accgcgagca aaggtctgga catattccgg atagagcacc gcctttgcct
     3721 gtacttcatt gcgcgcggta atcagatcca gcgtctggat ttgcccgata tcaataccga
     3781 gatagcgaat tggcatcccg accgccagtt ttccggcatc gaaagcatga agcgtaatct
     3841 gcccgccaac cgcacgggcc gctgtttcgg aagcatacag aatacgctta tcgcctttac
     3901 gctgactggc gctggcaccg ctgaggttat cgaagctaat ggctcccttt aatgctctgg
     3961 agagtgggga tgcctgtacg gtcaggccac taccattcag ctgaactttc gccccgcctt
     4021 ctgcccagaa cacgctattg ttggtcagaa ggttgcgata ctccggctta atatgcagat
     4081 cgatatcaaa cgcgttagct cgcggacgca cggtaataac ttcaccaact tcaaatttac
     4141 ggtacagcac taccgatcct gcctgcacat ccggcagcgt ctctgcactc aaactcacgg
     4201 ttgtggtggg taaatcgcta aggctgttct ccagcgcttt ttccagattg gcatacagtg
     4261 gatagctggc tttcatctca cctttatcgc ccggcagaat acgtatcccg ccattgatcc
     4321 attctgaggc gctggcaccg agaaactcaa cgccatccag ccccaccttc acatcgacac
     4381 ggctgttgac gacaaatttg ctatcgcctt ttaccagttc gcgatgctga ggctcaatgg
     4441 cgacggtaaa ggtaacgcct ttgctggtga gtttacgatc aataacctgg cctacctgca
     4501 cgccgtgaag aatgagcggc tgaccagcat caataccgta actttccggt gctgtcaggg
     4561 tcagtgtcag aacatcaggt tcatgcagca gtgctttttc gcctggcaca acaacgaact
     4621 ctttgcgtgg ctcgccatcg ccaggcacca actcaaaggt ttttccggtc agcagggcgc
     4681 tgagattggc atcgctaagg gataatttcg ggttgcgtaa ttcgatgcgg gtattttccc
     4741 gtaacagcgt aacgacgctg ggatcaacgg tcatttctcc ggtgacttta ccaccaggat
     4801 ttaaatccag tttagtcagc tgtccgactt ccagcccctg atacattaac ggcgtcgagt
     4861 cggcggttaa tccggcccca ctcggcagtt ccagttttat tattacgcca cgctggctgt
     4921 gggccagatc ttcatacaga ccaaaggtat cttccgcctc ggcaggtttc gactcttctg
     4981 gtgaatcgaa ggcaatcgca ccgttaacca gtgccgccag actttccagt ttcaccttcg
     5041 cgccactgat actgacgttg gcatcaacgc ctgaaacgtt ccagaaacgg ctaccttttt
     5101 tcaccaggtc ggtaaaacgc cgctcgatca ggacatcaat caccacgcct tgtttattgg
     5161 gattgatggc atagtcgtag acttttccca ccgggatctt gcggaaatag accaatgaac
     5221 cgctgttcag cgaaccgaga tcgggagctt gcaggtggat catcagatcg ccgttatcca
     5281 gccgatactt cggttgggta tcgagcgcga caaagtgatc ctgctcttta cctttacctg
     5341 gcatcatgcc gatatagtta ccaccgacga gggcgtccag cccggagaca cctgccaacg
     5401 atgcttttgg cgtcaccagc cagaactgag tctcttcgcg cagcgcatct ttcatatcgg
     5461 acttgatgct gaccttgact tcaatcttac gaagatcgtc gctgaggctg atatcctgca
     5521 ctgttccgac ttcaacgccc tgataacgaa caggcgtacg gcccgggaca ataccatccg
     5581 ccgacataaa gtcgatggtg acggtattac cccggtcctg ataactgtcc caaatcagcc
     5641 aaccggcaat cattagcgcg atgaacggca gcagccagaa aggtgagata cggcgtttat
     5701 ttttaatctg cgcttcagtc gtcgaagcgg gcgtttcctg actcatgtgc atcccaaagt
     5761 aagcggctgt ccagccattc cacagcaaga atagtcaaaa ttaccgctgc gccgaaataa
     5821 aacgcagccg gtcccatagt aaaagcgagg atctgatcgc gattaatcag cgacatggtt
     5881 aaagatatga caaacaggtc gagcatcgac cagcgaccaa tccaggtcac catccgcagt
     5941 aacagaatgc gtgtgcgtaa accttgctgg catttaaaat gaatgctcaa cagtaaagtg
     6001 aacatgacga tcactttagt aaacggtacc agaatactgg cgataaacac gattcctgca
     6061 accgcaatgt tgctacttgc cagcgacata attccggaaa gaattgtatc ttcctgccgt
     6121 cctccgttca gataaatgat agaaataggc aacaggttgg caggtaacaa taaaacgatt
     6181 gacgctaaca gcgccgccca gcatttttgc agactatgac ggcgacgcag gcgtagcgga
     6241 atatgacaac gcgggcagcg accacgctga tcaggatagc cggtaaaatg gcacccaaga
     6301 cagacacgaa gtttctcgtc cctacgcgta gcggggcgct gcggataaaa tcgctcccac
     6361 agctcctcga cattaagatg tgacaacgtc accgtcgtta aaatcaccaa cgccacaaaa
     6421 gagaacaagc cgacacccgc ctggatatgg gcataatcct gtacctttat agaagcaacg
     6481 ccaatgccga ccaggtagat atcgagcatt acccactctt taagtcgctc aagcatcagc
     6541 agtaccgggc gtagattcat tcccagtcgg ttaccaaacc ataaataagc tatggaggtc
     6601 accagaatga gcggggcacc aataacgcag aaaaagacca tcgaccccgt tatcgcatcg
     6661 ccctgtttgg tcatttgcca gatgccttgc ataacgttgg cgtcaatacg aatgcctaac
     6721 agccagatat gcaacagcgg ttcgccccag gcaaacggca tcagcaacag catggtgaag
     6781 gccattgccg ccaggcgcgt tagcgaccag tcgcgcccgt cacgaatttt tgcctgacag
     6841 cgcggacaat aggcactttg atgagaattt atctcgggca ggctaaacag catgtcacat
     6901 tgcgggcaac gttggtaatc accacgcggc agttcctcgc cgattgccct cactgttatc
     6961 ttttttgtcg gcgtaatttg tggtgtgtta agagccatga cgtgccaaat gtaataagaa
     7021 ttaacattaa tcttaaccca tgatgggaat gatcttgagc attaacgcat ttaatgctta
     7081 ttttaatagg ctggcagttc caggtaagac aaccaagtga ttatataatg aacaaaacag
     7141 aattttacgc ggatttaaat cgcgacttta acgcgctgat ggcgggagaa accagttttc
     7201 tggcaacgct tgcgaacacc agtgcgttgt tatatgagcg tctcactgac gtaaactggg
     7261 caggttttta tttgcttgag gacgatacac tggtgctcgg accatttcag ggcaaaattg
     7321 cctgtgtccg gatacccgtc gggcgtggcg tgtgcggcac tgcggttgcc cgcaatcaag
     7381 tgcagcgtat cgaggatgtt catgcgtttg acgggcatat tgcctgtgat gcggcgagta
     7441 attctgaaat tgttctgccg ctggtggtga aaaatcagat tattggtgtt ctcgacatcg
     7501 atagtaccgt cttcggtcgc tttacagacg aggacgagca aggcttacgt cagcttgtgg
     7561 cacagcttga aaaagtgctt gcaacgacgg attacaaaaa attctttgcg agcgtcgcag
     7621 gataatcaac ggataacgta gcatttactg atagcgtcat tataatgacg cctgttcatg
     7681 cctgcgcttg ttggctacgt ccgttgtaat caggaaattt catggaaaat caacctaagt
     7741 tgaatagcag taaagaagta atcgcgtttc tggccgaacg ttttccccac tgtttcagtg
     7801 cggaaggtga agcgcgtccg ctgaaaatcg gtatttttca ggatttggtc gatcgtgttg
     7861 ccggggaaat gaacctgagc aaaacgcaat tacgatccgc tttacgtctc tacacttcga
     7921 gctggcgtta cctttacggt gttaaacccg gcgcaacgcg tgtcgatctt gacggcaacc
     7981 catgcggtga gctggacgag caacatgtag agcatgctcg caagcagctt gaagaagcga
     8041 aagcgcgtgt tcaggcacag cgtgctgaac agcaagcgaa aaaacgcgaa gctgccgcag
     8101 ctgctggtga gaaagaagac gcaccgcgtc gcgaacgcaa gccacgtccg accacgccac
     8161 gccgcaaaga aggcgctgaa cgtaaacctc gtgcgcaaaa gccggtagag aaagcgccaa
     8221 aaacagcaaa agcacctcgc gaagaacagc acaccccagt ttctgacatt tcagctctga
     8281 ctgtcggaca agccctgaag gtgaaagcgg gtcaaaacgc gatggatgcc accgtattag
     8341 aaatcaccaa agacggcgtc cgcgtccagc tgaattcggg tatgtctttg attgtgcgcg
     8401 cagaacacct ggtgttctga aacggaggcc gggccaggca tgaacatgtt ttttaggctt
     8461 accgcgttag ctggcctgct tgcaatagca ggccagacct tcgctgtaga agatatcacg
     8521 cgtgctgatc aaattccggt attaaaggaa gagacgcagc atgcgacggt gagtgagcgc
     8581 gtaacgtcgc gcttcacccg ttctcattat cgccagttcg acctcgatca ggcattttcg
     8641 gccaaaatct ttgaccgcta cctgaatctg ctcgattaca gccacaacgt gctgctggca
     8701 agcgatgttg aacagttcgc gaaaaagaaa accgagttag gcgatgaact gcgttcaggc
     8761 aaactcgacg ttttctacga tctctacaat ctggcgcaaa agcgtcgttt tgaacgttac
     8821 caatacgctt tgtcggtact ggaaaagccg atggatttca ccggcaacga cacttataac
     8881 cttgaccgca gcaaagcgcc ctggccgaaa aacgaggctg agttgaacgc gctgtgggac
     8941 agtaaagtca aattcgacga gttaagcctg aagctggcag gaaaaacgga taaagaaatt
     9001 cgtgaaaccc tgactcgccg ctacaaattt gccattcgtc gtctggcgca aaccaacagc
     9061 gaagatgttt tctcgctggc aatgacggcg tttgcgcgtg aaatcgaccc gcataccaac
     9121 tatctttccc cgcgtaatac cgaacagttc aacactgaaa tgagtttgtc gctggaaggt
     9181 attggcgcag tgctgcaaat ggatgatgac tacaccgtta tcaattcgat ggtggcaggt
     9241 ggtccggcag cgaagagtaa agctatcagc gttggtgaca aaattgtcgg tgttggtcaa
     9301 acaggcaagc cgatggttga cgtgattggc tggcgtcttg atgatgtggt tgccttaatt
     9361 aaagggccga agggcagtaa agttcgtctg gaaattttac ctgctggtaa agggaccaag
     9421 acccgcactg taacattgac ccgtgaacgt attcgtctcg aagaccgcgc ggttaaaatg
     9481 tcggtgaaga ccgtcggtaa agagaaagtc ggcgtgctgg atattcctgg cttctatgtg
     9541 ggtttgacag acgatgtcaa agtgcaactg cagaaactgg aaaaacagaa tgtcagcagt
     9601 gtgatcatcg atctgcgtag caatggtggt ggggcgctga ccgaagcggt atcgctctca
     9661 ggtctgttta ttcctgcggg tcctattgtt caggtccgtg ataacaacgg taaagttcgt
     9721 gaagacagcg ataccgacgg acaggttttc tataaaggcc cgctggtggt actggttgac
     9781 cgcttcagtg cttcggcttc agaaatcttt gccgcggcaa tgcaggatta cggtcgtgca
     9841 ctggttgtgg gtgaaccgac gtttggtaaa ggcaccgttc agcaataccg ttcattgaac
     9901 cgtatttacg atcagatgtt acgtcctgaa tggccagcgc tgggttctgt gcagtacacg
     9961 atccagaaat tctatcgcgt taacggcggc agtacgcaac gtaaaggcgt aacgccagac
    10021 atcatcatgc cgacgggtaa tgaagaaacg gaaacgggtg agaaattcga agataacgcg
    10081 ctgccgtggg atagcattga tgccgcgact tatgtgaaat caggcgattt aacggccttt
    10141 gaaccggagc tactgaagga acataatgcg cgtatcgcga aagatcctga gttccagaac
    10201 atcatgaagg atatcgcgcg cttcaacgct atgaaggaca agcgcaatat cgtttctctg
    10261 aattacgctg tgcgtgagaa agagaataat gaagatgatg cgacgcgttt ggcgcgtttg
    10321 aacgaacgct ttaaacgcga aggtaaaccg gagttgaaga aactggatga tctaccgaaa
    10381 gattaccagg agccggatcc ttatctggat gagacggtga atatcgcact cgatctggcg
    10441 aagcttgaaa aagccagacc cgcggaacaa cccgcacccg tcaagtaata tcaatcaggc
    10501 acaagaaatt gtgcctgatt ttttaacagc ggcaagatgc cgtaaatcag atgctacaaa
    10561 atgtaaagtt gtgtctttct ggtgacttac gcactatcca gacttgaaaa tagtcgcgta
    10621 acccatacga tgtgggtatc gcatattgcg ttttgttaaa ctgaggtaaa aagaaaatta
    10681 tgatgcgaat cgcgctcttc ctgctaacga acctggccgt aatggtcgtt ttcgggctgg
    10741 tactgagcct gacagggata cagtcgagca gcgttcaggg gctgatgatc atggccttgc
    10801 tgttcggttt tggtggttcc ttcgtttcgc ttctgatgtc caaatggatg gcattacgat
    10861 ctgttggcgg ggaagtgatc gagcaaccgc gtaacgaaag ggaacgttgg ctggtcaata
    10921 ctgtagcaac ccaggctcgt caggcgggga tcgctatgcc gcaagtggct atctaccatg
    10981 cgccggacat caacgctttt gcaaccggtg cgcgccgtga tgcctctctg gttgctgtca
    11041 gcaccggttt gctgcagaac atgagcccgg atgaagccga ggcggtaatt gctcacgaaa
    11101 tcagccacat cgccaatggt gatatggtca ccatgacgct gattcagggc gtggtgaaca
    11161 ccttcgttat ctttatttcc cgtatcctgg cgcagcttgc cgcgggtttt atgggcggaa
    11221 atcgtgatga aggtgaagag agcaacggca acccgctgat ctactttgcg gttgcaacgg
    11281 ttctggaact ggtgtttggt attctggcga gcattatcac catgtggttc tcgcgtcatc
    11341 gtgaattcca tgctgatgcc ggttcggcaa aactggttgg tcgcgagaaa atgattgccg
    11401 cgctgcagcg cctgaaaacc agctatgaac cgcaagaagc aaccagcatg atggctttct
    11461 gcattaacgg taagtcgaaa tcgctcagtg agttgtttat gactcacccg ccgctggata
    11521 aacgtattga agctctgcgt acgggtgaat acctgaagta atatgaagta aatactaaaa
    11581 agcgcgtcct gctcgacgcg ctttttttat gccctggatc ttggctgagt gatacgtaac
    11641 ccactgatac aggcagcaat cactgccaga atagccgcag ccatcagcga gacgtgtgta
    11701 ccattatctc caaactgatt tagcatcagc gccaccagcg ccgcgccgct actctgaccc
    11761 agtagacgag ccgttcctaa catgccactg gctccaccgc tacgttcgcg aggcgcggag
    11821 gtaataatgg tgtggttatt gggtgactgg aacaagccaa atccagcgcc acataagatc
    11881 atcggccaga taatattgat atccgcaggt gacgcgggca gcagaaccag ggaaaaaagc
    11941 cccgcagcca tgatgaataa tcctaaagca cccagcaatc ctgcatgtac gcgttcaatc
    12001 aaatagcctg ccaacggagc catcaccatc gttgctaacg gccacggtgt cagaagtaaa
    12061 cctgtttcga cttcactacg cccgagcacg gtttgcaggt aaaagggcag ggaaaccatt
    12121 gccagcattt gtgcgcagaa agagcaaaca gatgtgcaaa tagaaagtga aaacagcggg
    12181 atacgcagta aatccaccgg tagcagcggt actggaagag aaagctggcg gcgaataaag
    12241 aaaataccaa cgacaaccat tacaaccagt tccgcagcaa ttaacgtcag cgattgcccc
    12301 tgagcgaaac cactcaacgc agtgataagc aggccgaagg ttaacgcgtt catcacggcg
    12361 ctgggcaggt cgaaacgggg tttactggcg cgagaaccat taggtggcag aaaacgcatc
    12421 gccagaagca gggcgataat acccaacggt acgttgatta aaaataacca tttccaggat
    12481 gcgatggaga ggattgctgc agcaattgtc ggcccggcag cagaagagac ggcaacaata
    12541 aacgagttta tgcccatccc tctacccaga aaacgttgtg gatagatcag gcggataagg
    12601 gcggtattaa cgctcatcaa cgctgcaccg ccgaaacctt gtatgacacg cgcaagggtg
    12661 agtatttgca gcgaatcaga aagggcgcag aacagtgaag acaacagaaa aacgaccaga
    12721 ccgcatttat aaatacgtcg atagccaaac atatcaccca gaaacgaaaa cgagagcagg
    12781 gagatgacaa tggcgatttg ataggcgttc actacccaga tggaactggc tggcgtggca
    12841 tgaaggtccg tggcgattgt tggcagggcg acgttggcga ttgcgccgtc aaggacagcc
    12901 atcgaaatac caatcacaat ggttaatatc gcaccgtatc gctggggcaa tggcaggccg
    12961 tcggcctgaa cttttggcat aggaatttta aatctttggt gaataatcaa caggggtatg
    13021 attctagcac tgatattcac ggtggatgtc gcagaattgt aactaaccgg caaaagatgg
    13081 attgcgacca ccctggtgcg aaattataat aaaaaacagt tctgattttt ataaaacact
    13141 cgcaatgagg tgataaatgg ctaacgcaga tctggataaa cagcctgatt ctgtatcttc
    13201 cgtgctaaaa gtttttggca ttttgcaggc gctgggtgaa gagcgcgaaa tagggataac
    13261 cgagctgtcg cagcgcgtca tgatgtcaaa aagcaccgtt tatcgctttt tacagaccat
    13321 gaaaacctta ggttatgtgg cgcaggaagg ggagtcggag aaatattccc tgaccctgaa
    13381 attgtttgaa ctgggcgctc gcgcgttaca aaacgtcgat ttaattcgta gcgcagatat
    13441 ccagatgcgt gagatctccc gcctgaccaa agaaactatc cacctcggcg cactggacga
    13501 agacagtatt gtttacattc acaaaattga ctctatgtac aatttgcgca tgtattcacg
    13561 gattgggcgt cgtaatccac tgtacagcac cgcgattggt aaggtactgc tggcatggcg
    13621 cgatcgcgat gaagtgaagc aaattcttga gggcgtggag tataaacgca gtaccgagcg
    13681 gaccatcacc agtacagaag cgttattacc ggttctggac caggtgcgcg agcaggggta
    13741 tggcgaagat aacgaagagc aggaagaagg gctgcgatgc attgcggttc cggtatttga
    13801 tcgctttggc gtggtcattg ccggtttgag catctccttc ccgacgttgc gtttctctga
    13861 agagcgttta caggaatatg tcgcaatgtt gcataccgca gcgcgcaaaa tttctgccca
    13921 aatgggttat cacgactatc cgttctgatg agagtaagaa cctgtcggaa tatcaaacag
    13981 acaggttctt tatttagcat gagaaaaata aagttgaagg tggcgttaga ttaaacgcag
    14041 ttgatataag aatattttac tcatgagtga gaatctggtt atttattaca cttaaccatt
    14101 atcgaccacg atattgcttt tgcgtaacag cgggcaatct gttatcccca aaaaaccact
    14161 tttagtgtgc aagtattgta ccgtgctggt gcctctggca gtcagatagg tacattgcaa
    14221 acctaattct gcggcattct ctttgcttcc aatcaaaacg ccatatccgc tgagtaataa
    14281 tcctatccat accagtgcta tcagcataac tgtgcgaatg atgaatcgca ttacaacctc
    14341 ttctcttttt tatgttcgct taatcgtagc ggcaatatgc gctgaagcaa gcgactcatt
    14401 ccgaaaaagc acgaatatcg acatagttag acgctgttta actaacgcat ggtagtttaa
    14461 tgacataagg taggtgaaac ggagattgga gtgaaaaagt ttcgatgggt cgttttggtt
    14521 gtcgtggtgt tggcttgctt gctgctttgg gcgcaggtat tcaacatgat gtgcgatcag
    14581 gatgtacaat ttttcagcgg aatttgtgcc attaaccagt ttatcccgtg gtgatatcat
    14641 tttttcagca ctagattttc ttcctctcca gcagtggtag aatagcgcct ctactcagag
    14701 gtggtgaaat gaacgaagtt gtaaattcag gcgtgatgaa cattgcgtct ttggttgtat
    14761 cggtggtggt tcttcttatc gggctcatct tgtggttttt tattaatcgt gccagttctc
    14821 ggactaacga acagattgaa ctgcttgagg cgttgctgga tcagcaaaaa cgtcaaaatg
    14881 cactgttacg tcgtttgtgc gaagcaaacg aaccagagaa agcagataaa aagactattg
    14941 agagtcaaaa atcggttgaa gacgaagata ttattcgcct ggtcgccgaa cgataatcct
    15001 tcctcaatct tgagtaaccc cgtcgttgaa gggttactca attcatcctg attatacgca
    15061 ctgtcatggt gcaactgctt caaattaaaa ctctggtct
//
LOCUS       DBFIFI010000098        15023 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.098, whole genome shotgun sequence.
ACCESSION   DBFIFI010000098 DBFIFI010000000
VERSION     DBFIFI010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15023
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.098"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            1..956
                     /gene="ybeQ"
                     /locus_tag="N7B39_002901"
                     /pseudo
     CDS             1..956
                     /gene="ybeQ"
                     /locus_tag="N7B39_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
     gene            complement(1026..1508)
                     /gene="ybeL"
                     /locus_tag="N7B39_002902"
     CDS             complement(1026..1508)
                     /gene="ybeL"
                     /locus_tag="N7B39_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HGU2422854.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            1744..4326
                     /gene="leuS"
                     /locus_tag="N7B39_002903"
     CDS             1744..4326
                     /gene="leuS"
                     /locus_tag="N7B39_002903"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HGU2422855.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVNTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPQWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            4341..4922
                     /gene="lptE"
                     /locus_tag="N7B39_002904"
     CDS             4341..4922
                     /gene="lptE"
                     /locus_tag="N7B39_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HGU2422856.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGAVSISQDTASVFRNGQTAEYQMVM
                     TVSASVLIPGRDIYPISTKVFRSFFDNPQMALAKDNEQEMIIKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            4922..5953
                     /gene="holA"
                     /locus_tag="N7B39_002905"
     CDS             4922..5953
                     /gene="holA"
                     /locus_tag="N7B39_002905"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HGU2422857.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYSQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            5955..6596
                     /gene="nadD"
                     /locus_tag="N7B39_002906"
     CDS             5955..6596
                     /gene="nadD"
                     /locus_tag="N7B39_002906"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HGU2422858.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCDDLLPEPVLTYINQQGLYR"
     gene            6620..7231
                     /gene="cobC"
                     /locus_tag="N7B39_002907"
     CDS             6620..7231
                     /gene="cobC"
                     /locus_tag="N7B39_002907"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HGU2422859.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            7491..7808
                     /gene="rsfS"
                     /locus_tag="N7B39_002908"
     CDS             7491..7808
                     /gene="rsfS"
                     /locus_tag="N7B39_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HGU2422860.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            7812..8279
                     /gene="rlmH"
                     /locus_tag="N7B39_002909"
     CDS             7812..8279
                     /gene="rlmH"
                     /locus_tag="N7B39_002909"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HGU2422861.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            8310..10211
                     /gene="mrdA"
                     /locus_tag="N7B39_002910"
     CDS             8310..10211
                     /gene="mrdA"
                     /locus_tag="N7B39_002910"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HGU2422862.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            10214..11326
                     /gene="mrdB"
                     /locus_tag="N7B39_002911"
                     /gene_synonym="rodA"
     CDS             10214..11326
                     /gene="mrdB"
                     /locus_tag="N7B39_002911"
                     /gene_synonym="rodA"
                     /EC_number="2.4.99.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HGU2422863.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            11337..12425
                     /gene="rlpA"
                     /locus_tag="N7B39_002912"
     CDS             11337..12425
                     /gene="rlpA"
                     /locus_tag="N7B39_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HGU2422864.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFIQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FANKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            12565..13776
                     /gene="dacA"
                     /locus_tag="N7B39_002913"
     CDS             12565..13776
                     /gene="dacA"
                     /locus_tag="N7B39_002913"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HGU2422865.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            13886..14149
                     /gene="ybeD"
                     /locus_tag="N7B39_002914"
     CDS             13886..14149
                     /gene="ybeD"
                     /locus_tag="N7B39_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HGU2422866.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            14250..14891
                     /gene="lipB"
                     /locus_tag="N7B39_002915"
     CDS             14250..14891
                     /gene="lipB"
                     /locus_tag="N7B39_002915"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HGU2422867.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
BASE COUNT         3695 a         3815 c         3927 g         3586 t
ORIGIN      
        1 gcgataattt atcaatagat gagattatcg aacgtgctga aaaaggcgat tgcgaggctc
       61 aatatattgt tgggttttat tataatcgcg atagcgcaat tgactctccg gacgacgaaa
      121 aggcctttta ctagctgaag ctggccgctg agcaaggtca ttgtgaagca cagtattcct
      181 tagggcaaaa gtataccgag gataaaagcc gtcataaaga taatgagcaa gccatcttct
      241 ggctgaaaaa agctgcccta caaggccata ctttcgcttc caacgccctt ggctggatac
      301 tggatcgtgg agaagacccc aactataaag aagcggttgc ctggtatcag atagccgcgg
      361 agagcggaat gtcttatgcg caaaataatc ttgggtggat gtacagaaat ggcaacggag
      421 ccgcacaaga ctatacgctg gcatttttct ggtacaaaca agctgcatta caaggccata
      481 gtgacgcgca aaacaatctg gccgatcttt atgaagacgg aaaaggcgtt gctcaaaacg
      541 agacactcgc cgcattctgg tatttgaaaa gcgcacagca gggtaatcgg catgctcagt
      601 ttcaaattgc atgggattat aacgctggcg aaggggtgga ccaggactat aagcaagcga
      661 tgtactggta tctgaaggct gccgctcagg ggagcgtcgg cgcttacgtc aacatcggtt
      721 atatgtataa acacggacaa ggcgttgaga aagattatca ggctgccttt gaatggttta
      781 cgaaagccgc tgaatgcaat gacgccactg cctggtataa cctggccatt atgtatcact
      841 acggagaagg aagacctgtc gatctccgac aggctctcga cctgtatcgt aaagttcagt
      901 catccggaac cagagatgtc agtcaggaaa ttcgtgagac tgaagattta ctgtagagat
      961 aaaaacaagg gccggggaat ttcattcggc ccatattctc tttattgctg tgcgaaatca
     1021 gactattacg gctcaaacgg gcgtctctgg aactggtcat gaccacattt cgggcatagc
     1081 gtcagcactt ccggtgtgta gatcgggaga tggaagtgac atttctcgca gaccagattt
     1141 cccagcccga ccacttctcc gctgtgataa accccatgat gattgaggtc ctggaaaact
     1201 tcgcgccatt caagctgcgt tttatcggtg atgtctgcca gctcctgcca caagctttct
     1261 ttaatcaccc gcataaagac gctgtcagat tcttctttca ggctctcttc atagctcatg
     1321 gcgaactctt ccaggtcacg tctgacagct cgcgtcagct catcgacctc ggttcgcgtt
     1381 aactcccctg tttttattac gcgctcgcgc gcctgttcca ccagtgcgtc gatatcacgt
     1441 tcgccattgc gcaggcgttc gctcagtgac gcaaccagtt cacggtaata ttgagcaacc
     1501 ttgttcatcg tttcatctcc cgggtcgtta actgactata ataatagacg ttctttgttc
     1561 cttgctttgc taatacgccc acaaactctg taaattaatg tatgaataat ccgggcatca
     1621 tttcaacgtc gagtgcgcga aaggctgttt tgacgcggac gtttgggcta tgctatgcgg
     1681 atctgaaaaa ccacctctaa cgctactttt gtagccgtaa tgaaaacagg accactggct
     1741 gccatgcaag agcaataccg cccggaagag atagaatcca aagtacagct tcattgggat
     1801 gagaagcgca catttgaagt aaccgaagac gagagcaaag agaagtatta ctgcctgtct
     1861 atgcttccct atccttctgg tcgactacac atgggccacg tacgtaacta caccatcggt
     1921 gacgtgatcg cccgttacca gcgcatgctg ggcaaaaacg ttctgcagcc gatcggctgg
     1981 gacgcgtttg gtctgcctgc ggaaggcgcg gcggtgaaaa acaacaccgc tccggcaccg
     2041 tggacgtacg acaacatcgc gtatatgaaa aaccagctca aaatgctggg ctttggttat
     2101 gactggagcc gcgagctggc aacctgtacg ccggaatact accgttggga acagaaattc
     2161 ttcaccgagc tgtataaaaa aggcctggta tataagaaga cttctgcggt caactggtgc
     2221 ccgaacgacc agaccgtact ggcgaacgaa caagttatcg acggctgctg ctggcgctgc
     2281 gataccaaag ttgaacgtaa agagatcccg cagtggttta tcaaaatcac tgcttacgct
     2341 gacgagctgc tcaacgattt ggataaactg gatcactggc cagacaccgt taaaaccatg
     2401 cagcgtaact ggatcggtcg ttccgaaggc gtggaaatca ccttcaacgt taacgactat
     2461 gacaacacgc tgaccgttta cactacccgc ccggacacct ttatgggttg tacctacctg
     2521 gcggtagctg cgggtcatcc gctggcgcag aaagcggcgg aaaataatcc tgaactggcg
     2581 gcctttattg acgaatgccg taacaccaaa gttgctgaag ctgaaatggc gacgatggag
     2641 aaaaaaggcg tcaatactgg ctttaaagct gttcacccat taacgggcga agaaattccc
     2701 gtttgggcag caaacttcgt attgatggag tacggcacgg gcgcagtaat ggcggttccg
     2761 gggcacgacc aacgcgacta cgagtttgcc tctaaatacg gcctgaacat caaaccggtt
     2821 atcctggcgg ctgacggctc tgagccagat ctttctcagc aagccctgac tgaaaaaggc
     2881 gtgctgttca actctggcga gttcaacggt cttgaccatg aagcggcctt caacgccatc
     2941 gccgataaac tgactgcgat gggcgttggc gagcgtaaag tgaactaccg cctgcgtgac
     3001 tggggtgttt ctcgtcagcg ttactggggc gcgccgattc cgatggtgac gctggaagac
     3061 ggtaccgtaa tgccgacccc ggacgaccag ctgccggtga tcctgccgga agatgtggtg
     3121 atggacggca ttaccagccc gattaaagca gatccacagt gggcgaaaac taccgttaac
     3181 ggtatgccag cgctgcgtga aaccgacact ttcgacacct ttatggagtc ctcctggtac
     3241 tatgcgcgct acacttgccc agagtacaaa gaaggtatgc tggattccga agcggctaac
     3301 tactggctgc cggtggatat ctacattggt ggtatcgaac acgccattat gcacctgctc
     3361 tacttccgct tcttccacaa actgatgcgt gatgcaggca tggtgaactc tgacgaacca
     3421 gcgaaacagt tgctgtgtca gggtatggtg ctggcagatg ccttctacta tgttggcgaa
     3481 aacggcgaac gtaactgggt ttccccggtt gatgctatcg ttgaacgtga cgagaaaggc
     3541 cgtatcgtga aagcgaaaga tgcggcaggc catgaactgg tttataccgg catgagcaaa
     3601 atgtccaagt cgaagaacaa cggtatcgac ccgcaggtga tggttgaacg ttacggcgcg
     3661 gacaccgttc gtctgtttat gatgtttgct tctccggctg atatgactct cgaatggcag
     3721 gaatccggcg tggaaggggc taaccgcttc ctgaaacgtg tctggaaact ggtgtacgag
     3781 catactgcga aaggtgatgt tgcggcactg aacgttgatg cactgactga agatcagaaa
     3841 gcgctgcgtc gcgatgtgca taaaactatc gctaaagtga ccgatgatat cggccgtcgt
     3901 cagaccttca acaccgcaat tgcggcgatt atggagctga tgaacaaact ggcgaaagca
     3961 ccgaccgatg gcgagcagga tcgcgctctg atgcaggaag cactgctggc cgttgtccgt
     4021 atgcttaacc cgttcacccc gcacatctgc ttcacgctgt ggcaggaact gaaaggcgaa
     4081 ggcgatatcg acaacgcgcc gtggccggtt gctgacgaaa aagcgatggt ggaagactcc
     4141 acgctggtcg tggtgcaggt taacggtaaa gtccgtgcta aaatcaccgt tccggtggac
     4201 gcaacggaag aacaggttcg cgaacgtgct ggccaggaac atctggtagc aaaatatctt
     4261 gatggcgtta ctgtacgtaa agtgatttac gtaccaggta aactcctcaa tctggtcgtt
     4321 ggctaagcgc gggaggaagc gtgcgatatc tggcaacatt gttgttatct ctggcggtgt
     4381 taatcaccgc cgggtgtggc tggcatctgc gtgacaccac gcaggttcct tccactatga
     4441 aggtcatgat cctggactca ggcgatccga acgggccatt aagccgtgcg gtgcgtaacc
     4501 agttacgtct gaatggtgtc gagttgcttg ataaagaaac cacgcgtaag gacgttccat
     4561 ccctgcgttt gggtgctgta tcaatctcac aggataccgc gtcggttttc cgtaatggtc
     4621 aaactgcgga atatcagatg gtgatgactg ttagcgcgtc ggtattgatt cctggacgag
     4681 atatctaccc gattagcacc aaagtcttcc gttcattctt cgataacccg caaatggcgt
     4741 tagcgaaaga taacgagcag gaaatgatca ttaaagagat gtacgaccgt gctgccgaac
     4801 agctgattcg taagctgcca agcatccgtg ctgcggatat tcgttccgac gaagaacaga
     4861 cgtcgactac aacggatact ccggcaacgc ctgcacgcgt ctccaccacg ctgggtaact
     4921 gatgattcgg ttgtacccgg aacaactccg cgcgcagctc aatgaagggc tgcgcgcggc
     4981 gtatctttta cttggtaacg atcctctgtt attgcaggaa agccaggacg ctgttcgtca
     5041 ggtagctgcg gcacaaggat tcgaagaaca ccacactttt tccattgatc ccaacactga
     5101 ctggaatgcg atcttttcgt tatgccaggc catgagtctg tttgccagtc gacaaacgct
     5161 attgctgttg ttaccagaaa acggaccaaa cgcggcgatc aatgaacaac ttctcacact
     5221 caccggactt ctgcatgacg acctgctgtt gatcgtccgc ggtaataaat taagcaaagc
     5281 gcaagaaaat gccgcctggt ttactgctct tgcgaatcgc agcgtgcagg tgacctgtca
     5341 gacaccggag caggctcagc ttccccgctg ggttgctgcg cgcgcaaaac agctcaactt
     5401 agaactggat gacgcggcga atcaggtgct ctgctactgt tatgaaggta acctgctggc
     5461 gctggctcag gcactggagc gtttatcgct gctctggcca gacggcaaac tgacattacc
     5521 gcgcgttgaa caggcggtga atgatgccgc gcatttcact ccttttcatt gggttgatgc
     5581 tttgttgatg ggaaaaagta agcgcgcatt gcatattctt cagcaactgc gtctggaagg
     5641 cagcgaaccg gttattttgt tgcgcacatt acaacgtgaa ctcttgttac tggttaacct
     5701 gaaacgccag tctgcccata cgccactgcg tgcgttgttt gataagcatc gggtatggca
     5761 gaaccgccgg ggcatgatgg gcgaggcgtt aaatcgctta agtcagccgc agttacgtca
     5821 ggccgtgcaa ctcctgacac gaacggaact caccctcaaa caagattaca gtcagtcagt
     5881 gtgggcagag ttggaagggt tatctcttct gttgtgccat aaacccctgg cggacgtatt
     5941 tatcgacggt tgatatgaaa tctttacagg ctctgtttgg cggcaccttt gatccggtgc
     6001 actatggtca tctaaaaccc gtggaaacgc tggcgaattt gattggtctg acgcgagtca
     6061 caatcatccc taataatgtt cctccgcatc gtccccagcc ggaagcgaac agcgtacagc
     6121 gtaaacacat gcttgaactg gcgattgccg acaagccatt atttactctt gatgaacgcg
     6181 agctaaagcg caatgccccc tcttacactg cgcaaacact gaaagagtgg cggcaggaac
     6241 aagggccgga cgtgccgctg gcgtttatta ttggtcagga ttcattgctg acctttccga
     6301 cctggtacga atacgaaacg atactcgaca atgcacattt gatcgtctgt cggcgtccag
     6361 gttacccact tgaaatggcg caaccgcaat accagcaatg gctggaagat catttgacac
     6421 ataacccgga agatcttcac cttcagcctg ccggtaaaat ttatctggct gaaacgccgt
     6481 ggtttaacat ctcggcgacc atcatccgcg aacgtttgca aaacggtgaa tcatgtgatg
     6541 atttattgcc ggaaccggta ctgacttaca ttaaccaaca aggcttgtat cgctgatacc
     6601 tgtcgttacg gagggcatga tgcgactgtg gttaattcgt catggcgaaa cacaagcgaa
     6661 tgtcgatggt ctgtacagcg gtcatgcgcc cacacccctg accgcacgcg gtattgagca
     6721 agcgcaaaat ctgcatacac tgctagatga tgtttccttt gacctggttt tatgcagtga
     6781 actggaacgg gcacagcata ccgcgcgact ggttctcagt gaccgccagc tccccgttca
     6841 tatcatccct gaactcaacg aaatgttttt tggcgactgg gagatgcgac atcatcgcga
     6901 cctcatgcaa gaagatgccg aaaactatag cgcgtggtgc aatgactggc agcatgctat
     6961 ccccacgaac ggtgaaggat ttcaggcatt ttcgcaacgt gtggaacgct ttatcgcaag
     7021 gcttagtgaa tatcagcact atcagaatat tttaatcgtc agccatcagg gcgtacttag
     7081 tctgttaatt gctcgtttaa ttggcatgcc tgccgagtct atgtggcatt ttcgcgttga
     7141 ccaggggtgt tggagcgcca ttgatataaa ccaaaaattc gcgacgctac gcgtcctcaa
     7201 tagccgtgcc atcggggtcg agaatgcatg acttttctgt ttttttacgg gtaagccgca
     7261 acgaccattg acagccccgg gcaggctgat attctccgca gccagacttt ttccgccaga
     7321 cacgactttg tagaaattgt tttacaaaaa tggcgatgca atctgcggcg cggggtggga
     7381 tgatagccca ctttcgaaag ccgattcggc gacaattgtc ccgaaatcgc ctctggttca
     7441 ggtatactga cagaccattt ttatctattt gattcaccca gggggaaaac ttgcagggta
     7501 aagcactcca ggattttgtt atcgacaaaa ttgatgacct caaaggtcag gacatcatcg
     7561 ccttagacgt tcagggcaaa tccagcatca ctgactgcat gatcatctgt acgggtacgt
     7621 ccagccgtca tgttatgtcc attgctgacc acgttgtgca ggagtctcgc gcagcgggcc
     7681 tgttaccgct cggagtagaa ggtgaaaaca gcgccgactg gattgtcgtg gatttgggcg
     7741 atgtgattgt ccatgtcatg caggaagaga gccgtcgcct gtatgaactg gaaaaactct
     7801 ggagttaatg cgtgaagctg caacttgtcg ccgtgggaac gaaaatgccg gactgggtac
     7861 aaaccggttt taccgagtac ctgcgtcgtt ttccgaaaga tatgcccttc gagctgattg
     7921 aaattccggc cggaaaacgc ggcaagaatg cggacatcaa gcgcatactc gacaaagagg
     7981 gtgagcagat gttggcggcc gcaggcaaaa accgcattgt caccctcgat attccaggca
     8041 agccctggga tacgccgcag ttagccgctg agctggaacg ctggaagctg gatggtcgcg
     8101 acgtcagtct actgattggc gggcctgaag ggttgtcgcc tgcctgtaaa gcggcggcgg
     8161 agcagagctg gtcgctgtcg gcgcttaccc tcccccatcc gctggttcgc gtgctggtcg
     8221 cagagagtct gtaccgggcg tggagcatca ccaccaacca tccttatcac cgtgagtgat
     8281 aagggagctt tgagtagaaa acgcagcgga tgaaactaca gaactctttt cgcgactata
     8341 cggcagagtc cgcgctgttt gtgcgccggg cgctggtcgc ctttttgggg attttgctgc
     8401 tgaccggcgt gcttatcgcc aacctgtata atctgcaaat tgttcgcttt accgactacc
     8461 agacccgctc taatgaaaac cgcattaagc tggtgcctat cgcgcccagc cgcggcatta
     8521 tctacgaccg taacggtatc cctctggccc tcaaccgcac tatctaccag atagaaatga
     8581 tgccggagaa agtcgataac gtgcagcaaa cgctggacgc tttgcgcagc gtggttgatc
     8641 tgaccgatga cgatattgct gcattccgaa aagagcgcgc acgttcacac cgtttcacct
     8701 ccattccggt gaaaactaac ctgaccgaag tacaagtagc tcgctttgcc gtcaatcagt
     8761 accgttttcc gggtgttgaa gttaaaggct ataaacgtcg ttactatcct tacggttcgg
     8821 cgttgaccca cgtcatcggc tatgtgtcga aaatcaacga taaagacgtc gaacgcctga
     8881 ataatgacgg caaactggcc aactatgcgg caacgcatga tatcggtaag ctgggcattg
     8941 agcgttacta tgaagacgtg ctgcacggtc agaccggtta tgaagaggtt gaagttaaca
     9001 accgtgggcg tgttatccgc cagttaaaag aagtcccacc gcaagccgga cacgatattt
     9061 acctgacgct ggatctcaaa ctccagcaat atattgaaac gctgctggcg ggtagccgcg
     9121 cagctgtggt agtcaccgat ccgcgtacag gtggggtgct ggcgctggtt tccacgccta
     9181 gttatgaccc aaacttgttt gttgacggta tctccagcaa agattattcc gccttgttga
     9241 acgacccgaa tacaccgctg gtgaaccgcg ccacacaggg ggtttatcct cccgcgtcta
     9301 cagttaaacc ctatgtggcg gtttcggcat tgagcgccgg ggtgatcacg cgcaatacga
     9361 cgctgtttga ccctggctgg tggcaactgc caggttcgga aaaacgttat cgtgactgga
     9421 aaaaatgggg ccacgggcgt ctgaatgtca caagatcgct ggaagaatct gcggatacct
     9481 tcttctatca ggtggcctac gatatgggga tcgatcgcct ctccgaatgg atgggtaaat
     9541 tcggttatgg tcattatacc ggtatcgacc tggcggaaga acgttccggc aatatgccta
     9601 cccgcgaatg gaaacagaaa cgctttaaaa aaccgtggta tcagggtgac accattccgg
     9661 ttggtatcgg tcagggttac tggacagcga ccccaatcca gatgagtaag gcactgatga
     9721 tcctgattaa tgacggtatc gtgaaggttc cgcatttgct gatgagcacc gccgaagacg
     9781 gcaaacaggt gccatgggta caaccgcatg aaccgcccgt tggcgatatt cattccggtt
     9841 actgggagct ggcgaaagac ggtatgtacg gtgttgctaa ccgccctaac ggtacggcgc
     9901 ataaatactt tgccagcgca ccgtacaaaa ttgcggcgaa atctggtacc gctcaggtct
     9961 tcggtctgaa agcgaacgaa acctataatg cgcacaaaat tgccgagcgt ttacgtgacc
    10021 acaaactgat gaccgccttt gcgccataca acaatccgca agtggctgtc gccatgattc
    10081 tggagaacgg tggcgcgggt ccggcggttg gtacgctgat gcgccagatc ctcgaccaca
    10141 ttatgctggg tgataacaac accgatctgc ctgcggaaaa tcctgcggtt gccgcagcgg
    10201 aggaccatta atcatgacgg ataatccgaa taaaaaaaca ttctgggata aagtccatct
    10261 cgatcccaca atgctgctga ttttactggc attgctggtt tacagcgccc tggttatctg
    10321 gagcgccagc ggtcaggata ttggcatgat ggagcgtaaa atcggccaaa tcgcgatggg
    10381 tctggtcatc atggtggtga tggcgcaaat tcctccacgc gtttatgaag gctgggcccc
    10441 ctatctctat atcatctgta ttattttgct ggtggcggta gatgctttcg gtgccatctc
    10501 taaaggcgct caacgctggc tggacctcgg tattgttcgt tttcagccgt cggaaattgc
    10561 caaaatagcc gtaccactga tggttgcgcg ctttatcaac cgcgacgttt gcccgccatc
    10621 gttgaagaac actggcatcg cgctggtgct gatatttatg cccacgctgc tggtggctgc
    10681 acagcctgac ctgggaacat caatcctcgt tgcgctttcc ggtctgtttg tactgttcct
    10741 ctctggcctt agctggcgtc tgattggcgt cgcagtagtg ctggtagcgg cgttcattcc
    10801 gattctgtgg ttcttcctga tgcatgatta ccagcgccag cgcgtaatga tgctcctgga
    10861 cccggaatca gacccactcg gcgcgggcta tcacattatt cagtctaaaa ttgctattgg
    10921 ctccggcgga ttacgcggca aaggctggct gcacggcact cagtcacagc ttgaatttct
    10981 ccccgaacgc catactgact ttatcttcgc ggtactggcg gaagagctgg gattagtggg
    11041 cattttgatt ctgctcgctc tctacattct gctgatcatg cgcgggctgt ggatagccgc
    11101 cagagcgcaa accacctttg gtcgcgtcat ggctggcggc ttaatgctga tattattcgt
    11161 ttatgtcttc gtaaatattg gtatggtaag cggtattctg ccggttgtag gggttccgct
    11221 cccactggtc agttatggag gatcggcgct aattgtgctg atggctgggt tcgggattgt
    11281 aatgtcaatc cacacccaca ggaaaatgtt gtcgaaaagc gtgtaagagg tgcgcaatgc
    11341 gtaagcagtg gctcgggatc tgcatcgcgg caggaatgct cgcggcatgt acaagcgatg
    11401 atggtcagca acagacagta agtgtaccgc agcctgcggt atgtaacggc cccatagttg
    11461 aaattagcgg ggcggacccg cgtttcgaac cactgaacgc gacggcaaat caggattacc
    11521 agcgcgacgg taaaagctac aaaatcgtgc aggatccgtc tcgatttatc caggcgggac
    11581 tggcggcaat ctatgatgcc gaaccaggca gtaacctgac ggcctctggc gaagctttcg
    11641 atccgacaca gctgacggcg gcccatccaa cgcttccgat ccccagctac gccagaatca
    11701 ctaacctggc taacgggcga atgatcgtgg tgcgcattaa tgatcgcggt ccttacggca
    11761 acgaccgcgt tatttcgctt tctcgcgcag cagctgaccg tcttaacacg tcaaacaaca
    11821 ccaaagttcg tatcgatccg attattgtcg cccaggatgg ttcgctttct ggtcctggta
    11881 tggcttgtac cacagtcgcc aaacagactt acgccctgcc tgcacctccc gatttaagcg
    11941 gtggcgcggg aacaagttca gtgtctggcc cgcagggtga cattcttccg gtcagtaatt
    12001 cgacgctaaa aagcgaagat ccgaccggcg cgccggtaac cagcagcggt ttcctcggcg
    12061 caccaacgac cttagcgcca ggtgtactgg aaggcagcga accgacgcct gctccacagc
    12121 ccgttgttac agctccgtcg acaacgcctg caacctcgcc tgcaatggtg acaccgcaag
    12181 ccgcctcgca aagcgccagc ggcaacttta tggtgcaggt cggggccgta agcgatcagg
    12241 ctcgtgcgca acagtaccaa cagcaactgg gacagaagtt cggcgtcccc ggtcgcgtaa
    12301 ctcaaaatgg cgcggtctgg cggatccagc ttggcccatt cgccaacaaa gccgaagcca
    12361 gtaccttgca gcaacgtttg caaaccgaag cccaattaca gtcatttatt accaccgcgc
    12421 agtagcgtaa agcaggcatc tgatgtgtca atttctgtaa atgacgttaa caaagtcacg
    12481 cggaaagtca gatgcctgcc ggtagtgcat ttgctatagt agggcacttt ttttaattcc
    12541 atcacggatg tcgtagttca gaccatgaat accatttttt ccgctcgtat catgaagcgc
    12601 ctggcgctca ccacggctct ttgcacagcc tttatctctg ctgcacatgc cgatgacctg
    12661 aatatcaaaa ctatgatccc gggtgtaccg cagatcgatg cggagtccta catcctgatt
    12721 gactataact ccggcaaagt gctcgccgaa cagaacgcag atgtccgccg cgatcctgcc
    12781 agcctgacca aaatgatgac cagttacgtt atcggccagg caatgaaagc cggtaaattt
    12841 aaagaaactg atttagtcac tattggcaac gacgcatggg ccaccggtaa cccggtgttt
    12901 aaaggttctt cgctgatgtt cctcaaaccg ggcatgcagg ttccggtttc tcagctgatc
    12961 cgcggtataa acctgcaatc gggtaacgat gcttgtgtcg ccatggccga ttttgccgct
    13021 ggtagccagg acgcttttgt tggcttgatg aacagctacg ttaacgccct gggcctgaaa
    13081 aatacccact tccagacggt acatggtctg gatgctgatg gtcagtacag ctccgcgcgc
    13141 gatatggcgc tgatcggcca ggcgttgatc cgtgacgtac cgaatgaata ctcgatctat
    13201 aaagaaaaag aatttacgtt taacggtatt cgccagctga accgtaacgg cctgttatgg
    13261 gataacagcc tgaatgtcga cggcatcaaa accggacaca ctgacaaagc aggttacaac
    13321 cttgttgctt ctgcgactga aggccagatg cgcttgattt ctgcggtgat gggcggacgt
    13381 acttttaaag gccgtgaagc cgaaagtaaa aaactgctaa cctggggctt ccgtttcttc
    13441 gaaaccgtta acccactgaa agtaggtaaa gagttcgcct ctgaaccggt ttggtttggt
    13501 gattctgatc gcgcttcgtt aggggttgat aaagacgtgt acctgaccat tccgcgtggt
    13561 cgcatgaaag atctgaaagc cagctatgtg ctgaacagca gtgaattgca tgcgccgctg
    13621 caaaagaatc aggtcgtcgg aactatcaac ttccagcttg atggcaaaac gatcgaacaa
    13681 cgcccgctgg ttgtgttgca agaaatcccg gaaggtaact tcttcggcaa aatcattgat
    13741 tacattaaat taatgttcca tcactggttt ggttaaaaat tgaacacttg aaagtgtaat
    13801 ttccgtcccc atatactaag catcagtaaa aaactcccgc cttctggcgg gagttgctat
    13861 ttaattacgt tacgccggag ctgacatgaa aaccaaactt aacgaactgc ttgaattccc
    13921 tactcctttt acttacaaag ttatggggca ggcgttacct gagctggttg atcaggtggt
    13981 tgaagtggta cagcgccatg cgccaggtga ctacacccca acggtaaaac caagcagcaa
    14041 aggcaactac cactcggtat ctatcactat caacgccact catatcgagc aggttgaaac
    14101 actgtatgaa gaactgggca aaatcgatat tgtccgcatg gttctgtaac tcgcttctcc
    14161 gttacccggc ccctggtcgg gtaactcccc tctcctcgct gtgatatact tttctcccac
    14221 ttttactcat tctccacgga gatgccgttt tgtatcagga taaaattctt gtccgccagc
    14281 tcggtcttca gccttacgag ccaatctccc aggctatgca tgaattcacc gatacccgcg
    14341 atgatagtac ccttgatgaa atctggctgg tcgagcacta tccggtattc acccaagggc
    14401 aggcaggaaa ggcggagcac attttaatgc ccggtgatat tccggtgatt cagagcgatc
    14461 gcggtgggca ggtgacttat cacgggccgg ggcaacaggt gatgtatgta ttgcttaacc
    14521 tgaaacgccg taaactcggt gtgcgtgaac tggtgacctt gcttgagcaa acagtggtga
    14581 ataccctggc tgaactgggt atagaagcgc atcctcgggc tgacgcgcca ggtgtctatg
    14641 ttggggaaaa gaaaatttgc tcactgggtt tacgtattcg acgcggttgt tcattccacg
    14701 gtctggcatt aaacgtcaat atggatcttt caccattttt acgtattaat ccttgtgggt
    14761 atgccggaat ggaaatggct aaaatatcac aatggaaacc cgaagcgacg actaataata
    14821 ttgcaccacg tttactggaa aatattttag cgctactaaa caatcctgac ttcgaatata
    14881 ttaccgctta atttcatacc tcaatggccc aatttacact gggtcattac tcataacacc
    14941 tgctaatgct ttacatctcc ttcgcgtttg tatattttct ttatcacttg cagaaatgcg
    15001 atgctacgcc ttgctccgta act
//
LOCUS       DBFIFI010000099        14900 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.099, whole genome shotgun sequence.
ACCESSION   DBFIFI010000099 DBFIFI010000000
VERSION     DBFIFI010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14900
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.099"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(38..2317)
                     /gene="maeB"
                     /locus_tag="N7B39_002916"
     CDS             complement(38..2317)
                     /gene="maeB"
                     /locus_tag="N7B39_002916"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HGU2422868.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            2606..3556
                     /gene="tal"
                     /locus_tag="N7B39_002917"
     CDS             2606..3556
                     /gene="tal"
                     /locus_tag="N7B39_002917"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HGU2422869.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            3576..5579
                     /gene="tkt"
                     /locus_tag="N7B39_002918"
     CDS             3576..5579
                     /gene="tkt"
                     /locus_tag="N7B39_002918"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HGU2422870.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALVRQKL
                     GWHHPPFEIPKDIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(5674..6717)
                     /gene="ypfG"
                     /locus_tag="N7B39_002919"
     CDS             complement(5674..6717)
                     /gene="ypfG"
                     /locus_tag="N7B39_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HGU2422871.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSDLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVVVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(6843..7418)
                     /gene="nudK"
                     /locus_tag="N7B39_002920"
     CDS             complement(6843..7418)
                     /gene="nudK"
                     /locus_tag="N7B39_002920"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HGU2422872.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLID"
     gene            complement(7486..9465)
                     /gene="aegA"
                     /locus_tag="N7B39_002921"
     CDS             complement(7486..9465)
                     /gene="aegA"
                     /locus_tag="N7B39_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HGU2422873.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDAALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHLTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLIRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            9671..11371
                     /gene="narQ"
                     /locus_tag="N7B39_002922"
     CDS             9671..11371
                     /gene="narQ"
                     /locus_tag="N7B39_002922"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HGU2422874.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGTPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLGRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYRSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            11535..14648
                     /gene="acrD"
                     /locus_tag="N7B39_002923"
     CDS             11535..14648
                     /gene="acrD"
                     /locus_tag="N7B39_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HGU2422875.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATAEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(14747..14806)
                     /gene="ypfM"
                     /locus_tag="N7B39_002924"
     CDS             complement(14747..14806)
                     /gene="ypfM"
                     /locus_tag="N7B39_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HGU2422876.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT         3479 a         3959 c         3903 g         3559 t
ORIGIN      
        1 ctgaatattc agggtaagcg tgagagttaa aaaagaatta cagcggttgg gtttgcgctt
       61 caaccacggc cagcgccacc atgttgacga tacgacgcac cgatgcgatc ggcgttaaca
      121 cgtgaaccgg tttcgccacg cccatcagca ccggaccgac agtcacacct tccgagctgg
      181 aaacgcgcag taagttgtaa ctaatgcggg cagcttccat gttcggcatc accagaatat
      241 tggcagaacc tttcaaaggg ctgtccggca tacggtcgtt gcgaatcgct tccaccagcg
      301 ctgcatcgcc gtgcatttca ccatcaatca tcagttctgg cgcacgttcc ctgaccagtt
      361 ccagcgcctg acgcattttg ctcgacgacg ggcagtcaga agaaccaaag ttggagtgcg
      421 acaacagagc aacgcgcggt tcaataccga aacgacggac agtttctgcc gccatcaagg
      481 tgatctccgc cagctcttct gcgtctggtt cgtcattgac gtaggtatcg gcaataaagg
      541 tgttaccact cggcagcagc agcgcgttca ttgcacctgc ggtgtgaacg ccatcgcgat
      601 aaccaaagac atttttcacc acgctaaaat gttcatgata atcacccacc gtaccgcaaa
      661 tcattgcatc ggcttcgcca cgctgaacca tgatcgcgcc gatcactgtc gggttactga
      721 tcagcgcccg ctgcgcctgt tcctgagtga cgccgcgacg cttcatgatc tggaagtatt
      781 cggtccagta ctctttaaag cgcggatcgg attcgttatt gacgatctca aaatcaacgc
      841 ccgctttgat ctgcaagccc agtttctgaa tgcgcatttc gatcacgttc ggacgaccga
      901 taaggatcgg tttcgccagt cccagcgtta ccagttcctg agtggcatgc agaacgcgcg
      961 cctcttcccc ttccggcaga actacgcgct tcggcgcttt gcgagcctgg gagaaaatcg
     1021 gcttcataaa caggttggtt ttgtaaacga actcagtcag cttgtcgatg tagacgtcga
     1081 aatcagcaat cggacgcgtc gccacacccg actccatcgc cgctttcgcg accgcaggag
     1141 caatcttgac tatcagacgc ggatcaaacg gttttggaat gatgtattca gggccaaagc
     1201 tcagatcctg atcgccatac gctgaagcca ccacttcact ctgttccgca tgggccagtt
     1261 ctgcaatcgc gcgtaccgcc gccagtttca tctcttcgtt gatggcggtt gcgccaacgt
     1321 ccagcgcgcc acggaagatg aacgggaagc aaaggacgtt gttcacctgg ttcgggtagt
     1381 cggaacggcc ggtacagatg atggcatccg gacgcacttc tttcgccagc ggcggcagaa
     1441 tttccggttc cgggttcgcc agcgccagga tcattggcgc tcgagccatt ttcttcacca
     1501 tttcctgggt cagcactttc gggccggaac agccgaggaa aatatctgcg ccttcaatca
     1561 catcatcgag ggtacgtttg ccgtcatcca ctaccgcata tgcggctttg gtttccgcca
     1621 tgtttggctc acggccctga tagataacgc cttttgaatc gcaaaccacg atgttatgtt
     1681 tttgcagacc caacgctacc agcaggttca tacaggcgat tgctgcagca cccgcgccgg
     1741 aaaccaccat acgcacgtca gagatgtttt tctccaccac gcgcaagccg ttgaggatgg
     1801 cggcagtgct gataattgcc gtgccgtgct gatcgtcgtg gaataccgga atattcatcc
     1861 gctcgcgcag tttctgttca atatagaaac attctggcgc tttaatgtct tcgaggttga
     1921 tgccgccgaa ggtcggttcg agcgcagcga cgacttcaat aaatttgtcc gggtcgagtt
     1981 catcaacttc aatgtcaaat acatcaatcc cagcgaattt cttaaacaga acgcccttgc
     2041 cttccatcac cggtttgcct gccagcgcgc caatgttgcc taaccccagc accgccgtac
     2101 cgttagagat caccgccacc aggttaccgc gcgcggtata tttgtaggct tttaacgggt
     2161 ctttttcgat ttcaagacaa ggtgcggcaa cgcctggtga gtaggccagc gccagatcgc
     2221 gctgcgttgc cagaggcttg gttggagaaa cctggatttt ccctggaact ggaaattcat
     2281 ggaaatcaag tgcactttgt tttaactggt catccatttg gttgttcctt tcacgtaacg
     2341 ttcacaaata aagtgtgtgg gcaacagccc ctgcccacaa cgtggcgcac attattacct
     2401 tgccggagtc tacagacttt gagcaagtcc aaactctcac cattaatata atgttttggt
     2461 aataatccta taacactgat gttacctgct taatccagca ataccatgcc tgtctgctat
     2521 gcttttttga tgcgtttagc gaaatttctc agaagtgtga attaacgcac tcatctaaca
     2581 ctttactttt caaggagtat ttcctatgaa cgagttagac ggcatcaaac agttcaccac
     2641 tgtcgtggca gacagcggcg atattgagtc cattcgccat tatcatcccc aggatgccac
     2701 caccaatcct tcgctgttac tcaaggctgc cggattatca caatatgagc atttaataga
     2761 cgatgctatc gcctggggta aaaaaaatgg caagacccag gaacaacagg tggtcgcagc
     2821 gtgtgacaaa ctggcggtca atttcggtgc tgaaatcctc aaaatcgtac ccggtcgcgt
     2881 gtcaacagaa gttgatgcac gcctctcttt tgataaagaa aagagtattg agaaggcgcg
     2941 ccatctggtg gacttgtatc agcaacaagg cgttgagaaa tcacgcattc tgatcaaact
     3001 ggcttcgacc tgggaaggaa ttcgcgcggc agaagagctg gaaaaagaag gtattaactg
     3061 caacctgacg ctgctgtttt cttttgcaca ggcgcgggcc tgtgcagaag cgggcgtttt
     3121 tctgatttcg ccgtttgtcg ggcgtattta tgactggtat caggcacgca agccgatgga
     3181 cccatatgtg gtggaagaag atccgggcgt taaatcggtg cgcaatatct acgactacta
     3241 taagcaacac cactatgaaa ccattgtgat gggcgcgagc ttccgtcgca ccgaacaaat
     3301 cctcgcctta accggctgcg atcgactgac tatcgcaccg aatttgctga aggagctgca
     3361 ggaaaaagtt tcgccagtgg tacgtaaatt aatcccacct tctcagacgt tcccacgccc
     3421 agctcccatg agcgaagcgg agttccgttg ggagcacaat caggatgcga tggcggtaga
     3481 aaaactgtct gaaggcattc gtctgttcgc cgttgatcaa cgcaaactgg aagatcttct
     3541 tgccgccaaa ctataaacca gccacggagt gttatatgtc ccgaaaagac cttgccaatg
     3601 cgattcgcgc actcagtatg gatgcggtac aaaaagccaa ttctggtcat cccggcgcgc
     3661 cgatgggcat ggctgatatt gccgaagtgc tgtggaacga ttttcttaaa cataacccta
     3721 ccgacccaac ctggtatgat cgcgaccgct ttattctttc caacggtcac gcgtcgatgc
     3781 tgctctacag tttgctgcat ctgaccggtt acgacctgcc gctggaagag ctgaagaact
     3841 ttcgtcaact gcattcgaaa acccctggcc acccggaaat cggctatacg cccggagttg
     3901 aaaccaccac cggtcctctt ggacaaggtt tggcgaacgc cgtcgggctg gcgatagcgg
     3961 aacgtacact ggcggcgcag tttaaccagc cggatcatga aattgtcgat cacttcacct
     4021 atgtgtttat gggcgacggc tgcctgatgg aaggtatttc ccacgaagtc tgttcgctgg
     4081 cgggcacgct gggactgggc aagctgattg gtttttacga tcacaacggt atttcgattg
     4141 atggagaaac cgaaggctgg tttaccgacg atacggcaaa acgttttgaa gcctatcact
     4201 ggcatgtgat ccatgaaatc gacggtcacg atccgcaggc ggtgaaggaa gcaatccttg
     4261 aagcgcaaag cgtgaaagat aagccgtcgc tgattatctg ccgtacggtg attggctttg
     4321 gttcgccgaa taaagcaggt aaggaagagg cgcacggcgc accgctgggg gaagaagaag
     4381 tggcgctggt acggcaaaaa ctgggctggc accatccgcc atttgagatc cctaaagata
     4441 tttatcacgc ttgggatgcc cgcgaaaaag gcgaaaaagc gcagcagagc tggaatgaga
     4501 agtttgccgc ctataaaaag gctcatccgc aactggcaga agagtttacc cgtcggatga
     4561 gcggtggttt accgaaggac tgggagaaaa cgactcagaa atatatcaat gagttgcagg
     4621 cgaatccggc gaaaatcgct acccgtaagg cttcgcaaaa tacgcttaac gcttacgggc
     4681 cgatgctacc ggagctgctc ggcggttcgg cggatctggc tcccagcaac ctgaccatct
     4741 ggaaaggttc tgtttcgctg aaggaagatc cggcgggcaa ctacattcac tacggggtgc
     4801 gtgaatttgg catgaccgct atcgccaacg gcattgcgca ccacggcggc tttgtgccgt
     4861 ataccgcaac gttcctgatg tttgttgaat acgcccgtaa cgccgcgcgg atggcggcac
     4921 tgatgaaagc gcggcagatt atggtttata cccacgactc aattggtctg ggcgaagatg
     4981 gtccgacgca ccaggctgtt gagcaactgg ccagcctgcg cttaacgcca aatttcagca
     5041 cctggcgacc gtgcgatcag gtggaagcgg cggtgggctg gaagctggcg gttgagcgcc
     5101 acaacggacc gacggcactg attctctcca ggcagaatct ggcccaggtg gaacgtacgc
     5161 cggatcaggt taaagagatt gctcgtggcg gttatgtgct gaaagacagc ggcggtaagc
     5221 cagatattat tttgattgcc accggttcag agatggaaat caccctgcaa gcggcggaga
     5281 aattagcggg agaaggtcgc aatgttcgcg tggtttccct gccctcgacc gatattttcg
     5341 acgcccagga tgaggaatat cgggagtcgg tgttgccttc taacgttgcg gctcgcgtgg
     5401 cggtggaagc aggtattgcc gattactggt acaagtatgt tggtctgaaa ggggcaattg
     5461 tcgggatgac gggttatggg gaatctgctc cggcggataa gctgttcccg ttctttggct
     5521 ttaccgccga gaatattgtg gcaaaagcgc ataaggtgct gggagtaaaa ggtgcctgat
     5581 ggtgatcgcc ggatgctgat tgccggatgc gacgctgacg cgtcttatcc ggcctacagc
     5641 gtcttatccg gcctacatgt cccgccattt tgtttaacgg gtgatccaca acgtgggcca
     5701 ggcgtctggc ccatgccagt tatcgcaggt gggttctgcg gcgtaacgca ccaggcgaaa
     5761 acgctgaccg tcaaagcgcc agcgcgcctg aatgccacaa tcgcttaatc cgcgcccttt
     5821 cgctaaggtc accagttcac gcgatttctc atcaaatgtt gcgttcatca gttccagttc
     5881 attcgtctcc tgaccgttgt tgaacggcaa acgcagccgg accgggcgcg aggccagcgg
     5941 ctttttacgc gacacaatcc atgccaaatc aatggtgtta taggcccctg cctcacagct
     6001 aatcatcatc agcgctttat catcagtcag cgcagtgaca ttcacctcac gacgcaatgg
     6061 atcaagcgag cagcgcagac cattcatccg ccagtttcca taatccagca aatcgttgcg
     6121 ttcttcgagt gagagtggcg tcggcgttgg gttaaccacc acgacctctt tcagcgcagg
     6181 cgcgggcggt acgctgagcg gcggttcgtc ccctttcttg atccacgcgg tttcactgcc
     6241 aacgcgcttt tgctgagcat caataaacaa caacgccgct tttaagtcac tcagagaaat
     6301 ggtctgattg ccgtcgcgta aggtgatcgc cttcccttcc tgaatcatct gcaaaaacgc
     6361 ggtgatggtt gccgtatcat cggttactaa taaccatggt gaaatccgcc acttgtcacc
     6421 acttaacgcc aacggctcgc catctaacag cagccgtggc gcaatttccc cttcagacgc
     6481 atccggcgac ttcaacccgc cgcgctcaat acgtaaaacg gcatcggtat gcgccccggc
     6541 gctgcggctc agggtcatca ccagtccatt atgatcgccc gtattacgcg ccacgcagaa
     6601 gttttggtta ttacaggtga cctgccagtc ggaaaacgcc cgttgcgctg gtgccgccca
     6661 caccaaagac gtcggcaaca aagcgaaaaa aaagagaagg aaaatgcgat agcgcatgga
     6721 cggtacgacc ccagaagttc aaacaaacag cacaagtcgt atcttcgttg cgcaggtagc
     6781 gcggctcaat cggatttgtc ggattgagcc aagaataaac aacgaaatat tattgttatt
     6841 tttcagtcta ttaaatgtga catttgcaaa tagttaagca ataacaccgt cttaccgtca
     6901 cgtatctcgc cggttttgat catctccagc gcctggctga acggcagctc gagcacttca
     6961 atatcttcat cttcgacacc gccaccggcg ttagcgcgct gattgtcact gtattcggcg
     7021 ataaaaaagt ggattagctc agtcacaccg cctggcgaca tatacagttc aaataatttg
     7081 cgcacttcgc ccacttcata gcccgtctct tcaatcgctt ctttgcgaat gcacacttcc
     7141 ggttcgtcgt tatccagcag cccggcgcag gtttcaatca gctgcccgct ttcattgcca
     7201 ttaacccagg tagcgacacg gaactgacga atcagaacca cggtcttttt cttcgcgttg
     7261 tacaggagga tcgtcgcacc attaccgcga tcgtaaactt cacgtttatg gcggataact
     7321 tcgccatctt tgcgcgtgag atcgtaagta atgttgtgca gggtgaaata gttatcggag
     7381 agaattttgt ctttaatgag ggtgatttgt tgcgtcatac cgactccaca gcgcgaaatg
     7441 aacaattata ttacgctgtg aagtcgggtt tgtctgcgca ggctatcagt gagatttgac
     7501 tgattttacc cccagccagt caataatccc ctgtgccgca tgacgtcctt ctgccattgc
     7561 ggtaaccacc agatccgcac cacgcacggc gtcgccaccg gcgaagattt tcggattggt
     7621 ggtctggtaa cggtactggc tttccacatc cgcgatgatg cggccccatt tgtctaccgt
     7681 tacaccgtgc gactccagcc acggcatccc gtgcggattg aagccaaacg ccataatcac
     7741 cgcgtcggct ggcatgacaa attcactgcc ttccaccggc actggacgcc gacgcccctg
     7801 ggcatccggc tcgccaagac gcgtgcgcag gaaacgaatc ccgcagacgt gaccttgttc
     7861 attcagctca agcgccaccg gctggacgtt aaattcgaag ttggccccct cttcgcgggc
     7921 gttcttcacc tctttcttcg agcctggcat gttagcttca tcacgacgat aagcgcaggt
     7981 gacgttactc gcgccgtggc gcagcgcagt acgcacacag tccatcgcgg tgtcgccgcc
     8041 gcccagtacc acgacgttaa gtccggcggt attgataaac ggctcttccg gtagctcttc
     8101 gagccccatc acctgtttag tgttggcgat gaggaacggc agcgcgtcat aaacgcccgg
     8161 cgcatcttca ttgggtaaac ccgctttcat ggaacggtaa gtgcctacgc caacgaagac
     8221 cgcgtcgtat tgttccaaaa gcgaatccaa agagacatct ttacccactt cacaattgag
     8281 ttcgaagtga atccccatcg cgctgaagat ttcccgacga cgtgccagca gggatttatc
     8341 cagtttgaaa gaaggaatgc cgaaagtgag caagccaccg atttctggat ggcgatcgta
     8401 caccgtcacc gccacgccat tgcggatcag aacatccgca caggccagcc ctgccggacc
     8461 tgcaccgata atcgccaccc gcttgtccac tttggtgaca tggcttaagt caggacgcca
     8521 acctttcgcc aacgcctgat ctgaaatgta gcgttcaatg ttgccgatag ttaccgcacc
     8581 gtgctcatcg cgaatagtac aggcaccttc acacaaacgg tcttgcggac aaacgcgtcc
     8641 ggtaatttcc ggcagggtgt tggtcaggtg agaaagctcg acggcggcgt cgatgtttcc
     8701 ggctttcacc agttcaatcc actgcggtat atggttatgc agcgggcagg tccattcaca
     8761 aacgctgtgc tcgccgcatt taaggcagcg cgaggcttcc cgttgtgcct gatcggcacg
     8821 gaatggcaga taaatttcat caaaaccggt tttgcgcgct tcaatcgcca gtttatccgg
     8881 ctcgccacgc gcgggcgttg cctgcatttg ttcgacttta ctcattaccg gcatttcttg
     8941 tgcagcggta ctggcatgcc acggttgatg ttcctgacgt gcggtacgca agcggcggga
     9001 tttcgccatg cctgagagtg cggcgtcagt gaccagctgc agcgcgtccg ccgggcaatt
     9061 ctcgacacag gcaggaccgt tttcgcgccc tgcacaaagg tcgcatttat gcgccgtggc
     9121 tttaactttg cccgctgcga cgggtgtcag gacgatttgc atcgtaccaa acggacaggc
     9181 caccacgcag gatttacagc caatacactt ttgctgattg acctgaatgc tgtcatcaac
     9241 gtggctgatt gcgccattag ggcagctacg ggcgcagggc gcatcttcac aatggtgaca
     9301 ggtcactgca ctacgttgct gctgatgttt gataaccgta attcggggat gaaaatggtg
     9361 ttggctcagg acatgttgct catcattgtg agccatgaca caagcgattt cacaagcatg
     9421 acaacccaga cattgctgac tgttggccat aataaaacga ttcataacga ccttcttttt
     9481 tggttgtaaa aaccttattc tttatatgag tgttgttatt acccgactta caggggaatc
     9541 ggcaatgttc atgtgcccag aataagtaac tatttcgcta taaactgtgg cagatcaaat
     9601 aatcccatca ctaactaaat tgcgtttcag cgaactggaa cattaatgat tttttgtgga
     9661 gaagacgcgt gtgattgtta aacgacccgt ctcggccagt ctggcccggg ccttttttta
     9721 cattgtgctg ctgtcgattc tttccacggg tatcgctctg ctaactctgg cgagcagttt
     9781 gcgcgacgct gaggctatca atattgccgg atcgctgcgt atgcagagtt accgcctggg
     9841 ctacgacttg caaagtggca ctccacaact caatgcacat cgccagctgt ttcagcaggc
     9901 actgcattca ccggtattaa ccaacctcaa cgtctggtat gtgccagaag cagtaaaaac
     9961 tcgctatgcg catctgaatg ccaactggct ggagatgaat aatcggctca gcaagggcga
    10021 tttgccgtgg tatcaggcca atattaataa ttatgttaat cagatagacc tgttcgtgct
    10081 ggctttacag cactacgctg aacgcaaaat gctgctggtg gtggcgattt ccctggctgg
    10141 cggtatcggt attttcacgc tggtcttttt tactttgggc cgcatacgcc atcaggtggt
    10201 tgccccgctg aatcagctgg ttaccgccag tcagcgtatt gaacacggac agttcgactc
    10261 gccaccgctg gataccagcc tgccgaatga gcttggtcta cttgcaaaaa cctttaacca
    10321 gatgtcgagc gagctgcata aattgtaccg ttcgctggag gcgtcagtag aagaaaagac
    10381 ccgcgatctc cacgaggcca agcgtcgtct ggaggtgttg tatcaatgtt cgcaggcgct
    10441 gaacaccagc cagattgatg tgcattgttt ccgccatatt ttgcagattg ttcgcgacaa
    10501 tgaagcggct gaatatctgg agttaaatgt cggtgaaaac tggcggatta gcgaagggca
    10561 accaaacccg gaattgccga tgcagatttt accggtgaca atgcaagaga cggtttacgg
    10621 cgaactgcac tggcaaaata gtcacgtttc atcatcagaa ccgctgctta acagcgtttc
    10681 gtcgatgctg ggacgcggtt tgtactttaa tcaggcgcag aagcattttc agcagttatt
    10741 gttgatggaa gaacgtgcaa ccatcgcccg cgaattgcac gactcgctgg ctcaggtact
    10801 ttcttactta cgtatccagt tgacgttact taagcgttcg ataccggaag ataacgccac
    10861 cgcacaaagt atcatggccg atttttccca ggcgttgaat gatgcttatc ggcagttacg
    10921 cgagctgttg actacttttc gcctgacgct gcagcaggcg gatctcccct ccgcgttgag
    10981 ggaaatgctg gatacgttac aaaatcaaac cagcgccaaa ctgaccctcg actgccgtct
    11041 gccaaccctg gcactggatg cgcaaatgca ggtgcatttg ttgcaaatta ttcgcgaagc
    11101 ggtgctgaat gcgatgaagc acgccaacgc cagcgaaatc gccgtcagtt gcgtcaccgc
    11161 gccggacggc aatcacacgg tttatatccg tgataacggg attggtatcg gtgaaccgaa
    11221 agaacccgaa ggtcattatg gtctgaatat catgcgcgaa cgcgcggaac ggctaggtgg
    11281 gacgctgact ttttcacaac cttccggcgg cggcacgtta gtgagtatta gctttcgctc
    11341 tgcggagggt gaggaaagtc agttgatgta atgcctccta ctgaccaaag aatacttgca
    11401 gttaaggttc agtataaaag ggcatgataa tttacattaa ctcctttttt tctccacgat
    11461 tggctcgtac cttgccgcta cagtgaagca agtcaagcct acaacgatac gcagaaacac
    11521 gaggtcctct tttaatggcg aatttcttta ttgatcgccc catttttgcc tgggtgctgg
    11581 caatcctgtt gtgtctgaca ggtaccctgg cgattttttc attgcccgtt gaacaatacc
    11641 ccgatctcgc gccaccgaat gtgcgagtga ccgctaacta tcccggcgca tcggcccaga
    11701 cgctggaaaa caccgtgacc caggttatcg agcaaaatat gaccggcctc gataatctca
    11761 tgtatatgtc atctcagagc agtggcaccg gtcaggcatc tgtcacttta agttttaaag
    11821 caggcaccga tccggacgaa gccgtgcagc aagtacaaaa ccagctgcaa tcagccatgc
    11881 gaaagttacc gcaggcggtg caaaatcagg gcgtgactgt gcgtaaaacc ggcgatacca
    11941 acattctgac cattgccttc gtctctaccg atggttcgat ggataaacag gatattgctg
    12001 attatgttgc cagtaatatt caggacccgt taagccgcgt aaatggcgtc ggggatatcg
    12061 atgcctatgg ttcgcaatat tccatgcgta tctggctgga cccggcgaaa ctcaacagtt
    12121 tccagatgac agctaaagat gtcactgatg ccattgagtc acagaacgcg cagattgcgg
    12181 ttgggcaact tggtggtaca ccttccgtcg ataagcaggc gctcaacgcc accattaacg
    12241 cccagtcact gctgcaaaca ccagaacagt tccgcgatat caccttgcgg gtgaatcagg
    12301 acggctcaga agtaaggctc ggcgatgtcg ccaccgccga aatgggggcg gagaaatacg
    12361 attatcttag ccgcttcaat ggtaagccag cctccgggtt gggggtaaaa ctggcctccg
    12421 gcgcgaacga aatggcgaca gcggagctgg tgctcaatcg tctcgacgag ctggcgcagt
    12481 atttcccgca cggactggaa tacaaggtgg cgtatgaaac cacctcgttt gttaaagcct
    12541 ccattgaaga cgtggtgaaa acgctgctgg aagctatcgc tctggttttc ctcgttatgt
    12601 atctgttcct gcaaaacttc cgcgccacgc tgatacccac tatcgccgtg ccggtggtgt
    12661 tgatgggaac cttctccgta ctttacgcct tcggttacag cgtcaacacc ttaaccatgt
    12721 tcgcgatggt gctggcgatc ggtctgctgg tggatgacgc catcgtggtg gtggaaaacg
    12781 tcgaacgtat tatgagtgag gaaggactca ctcctcgcga agccacgcgt aaatcgatgg
    12841 ggcagatcca gggggcactg gtcgggattg cgatggtgct ttcggcggta tttgtaccaa
    12901 tggccttctt cggcggcacc accggtgcca tctatcgcca gttctctatt accattgttg
    12961 cggcgatggt gctgtcagta ctggtagcga tgatcctcac tccggctctg tgtgccacac
    13021 tacttaagcc actgaaaaaa ggtgagcatc acgggcaaaa aggctttttt gcctggttta
    13081 accagatgtt taaccgcaac gccgaacgct acgaaaaagg ggtggcgaaa attctccacc
    13141 gtagcctgcg ctggattgtg atttatgtcc tgctgcttgg cggcatggtg ttcctgttcc
    13201 tgcgtttgcc gacgtcgttc ttaccgctgg aagaccgtgg catgtttact acctcggtac
    13261 aattgcccag cggttcaacc caacaacaga ccctgaaagt cgttgagcaa atcgagaaat
    13321 attacttcac ccatgaaaaa gacaacatca tgtcggtgtt tgccactgtt ggttctggcc
    13381 ctgggggtaa cgggcaaaac gtggcgcgaa tgtttatccg cctgaaagac tggagcgaac
    13441 gcgacagtaa gaccggcacc tcgtttgcca ttatcgagcg tgcaacaaag gcgtttaacc
    13501 aaattaaaga agctcgcgtt atcgccagca gcccgccagc aattagcggt cttggtagtt
    13561 ctgcaggttt tgatatggag ttgcaggacc acgctggagc aggtcacgat gcgctgatgg
    13621 cagcacgtaa ccagttgctg gcgctggcgg cggaaaaccc ggagctaacc cgtgtgcgcc
    13681 ataacggcct cgatgacagt ccgcagttgc agattgatat cgaccagcgt aaagctcagg
    13741 cgctgggcgt tgctatcgac gatattaacg acacactgca aaccgcctgg ggttcgagct
    13801 atgtgaatga ctttatggat cgcggtcgcg tgaagaaagt ctatgtgcag gcagctgcgc
    13861 cgtatcgcat gctgccagat gacatcaatc tctggtatgt ccgaaataaa gatggcggca
    13921 tggtgccctt ctctgctttc gcgacctcac gctgggagac aggctcgccg cgtctggaac
    13981 gctataacgg ttattctgcg gttgaaattg ttggggaagc cgcaccgggg gtcagtaccg
    14041 gtacggcgat ggatattatg gaatcgttag tgaagcagct accaaacggc tttggtctgg
    14101 agtggacggc gatgtcgtat caggagcggc tttccggcgc gcaggctccg gcgctgtacg
    14161 ccatttcctt gctggtggta ttcctgtgtc tggctgcatt gtatgaaagc tggtcggtac
    14221 cgttctcggt aatgctggtc gtgccgctgg gggtaatcgg cgcgctgctg gcaacctgga
    14281 tgcgcgggct ggaaaacgac gtttacttcc aggtgggcct gttaacggtc attggtttat
    14341 cggcgaaaaa cgccatcctg atcgtcgagt ttgctaacga gatgaaccaa aaaggccacg
    14401 acctgtttga agcgacgctc cacgcctgcc gtcagcgttt acgcccgatt ctgatgacct
    14461 cgctggcatt tatcttcggc gtattgccaa tggcaaccag cacgggtgcc ggttccggtg
    14521 gtcagcatgc ggtgggtact ggcgtaatgg gcgggatgat ttcagccact attctggcta
    14581 tttacttcgt gccgctgttc tttgtgctgg tgcgccgccg cttcccgctg aagccgcgcc
    14641 cggaataagc aataaaaaag gcgacatgcc aatgtgtcgc ctttttcaac tttccgatta
    14701 agaacctgct cagcgggttc ttgctgtttg tactttgtct caggaattac ttacgaagca
    14761 taacttcaat aaagtctttc cagttcccca gttcacgttc aatcataaca acctctctta
    14821 taattatggg tattctacgg aaacaatata ccgtggtgaa gctaatttac tcgattgctg
    14881 cgatgactac ctccggggga
//
LOCUS       DBFIFI010000100        14900 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.100, whole genome shotgun sequence.
ACCESSION   DBFIFI010000100 DBFIFI010000000
VERSION     DBFIFI010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14900
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.100"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(331..1422)
                     /gene="ychF"
                     /locus_tag="N7B39_002925"
     CDS             complement(331..1422)
                     /gene="ychF"
                     /locus_tag="N7B39_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HGU2422877.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1539..2123)
                     /gene="pth"
                     /locus_tag="N7B39_002926"
     CDS             complement(1539..2123)
                     /gene="pth"
                     /locus_tag="N7B39_002926"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HGU2422878.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2401..2679
                     /gene="ychH"
                     /locus_tag="N7B39_002927"
     CDS             2401..2679
                     /gene="ychH"
                     /locus_tag="N7B39_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HGU2422879.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2734..4413)
                     /gene="dauA"
                     /locus_tag="N7B39_002928"
     CDS             complement(2734..4413)
                     /gene="dauA"
                     /locus_tag="N7B39_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HGU2422880.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4538..5485)
                     /gene="prs"
                     /locus_tag="N7B39_002929"
     CDS             complement(4538..5485)
                     /gene="prs"
                     /locus_tag="N7B39_002929"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HGU2422881.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5636..6487)
                     /gene="ispE"
                     /locus_tag="N7B39_002930"
     CDS             complement(5636..6487)
                     /gene="ispE"
                     /locus_tag="N7B39_002930"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HGU2422882.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGANLS
                     PLHRAML"
     gene            complement(6487..7110)
                     /gene="lolB"
                     /locus_tag="N7B39_002931"
     CDS             complement(6487..7110)
                     /gene="lolB"
                     /locus_tag="N7B39_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HGU2422883.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7324..8580
                     /gene="hemA"
                     /locus_tag="N7B39_002932"
     CDS             7324..8580
                     /gene="hemA"
                     /locus_tag="N7B39_002932"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HGU2422884.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSDIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEQVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8622..9704
                     /gene="prfA"
                     /locus_tag="N7B39_002933"
     CDS             8622..9704
                     /gene="prfA"
                     /locus_tag="N7B39_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HGU2422885.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9704..10537
                     /gene="prmC"
                     /locus_tag="N7B39_002934"
     CDS             9704..10537
                     /gene="prmC"
                     /locus_tag="N7B39_002934"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HGU2422886.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALARERPDCEITAVDRMPDAVALAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10534..10926
                     /gene="sirB2"
                     /locus_tag="N7B39_002935"
     CDS             10534..10926
                     /gene="sirB2"
                     /locus_tag="N7B39_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HGU2422887.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLTLVVLYIIIKLATTKVPLLG"
     gene            10930..11739
                     /gene="sirB1"
                     /locus_tag="N7B39_002936"
     CDS             10930..11739
                     /gene="sirB1"
                     /locus_tag="N7B39_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HGU2422888.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            11775..12629
                     /gene="kdsA"
                     /locus_tag="N7B39_002937"
     CDS             11775..12629
                     /gene="kdsA"
                     /locus_tag="N7B39_002937"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HGU2422889.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLRQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(12777..12884)
                     /gene="ldrA"
                     /locus_tag="N7B39_002938"
     CDS             complement(12777..12884)
                     /gene="ldrA"
                     /locus_tag="N7B39_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HGU2422890.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(13313..13420)
                     /locus_tag="N7B39_002939"
     CDS             complement(13313..13420)
                     /locus_tag="N7B39_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HGU2422891.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(13819..>14900)
                     /gene="chaA"
                     /locus_tag="N7B39_002940"
     CDS             complement(13819..>14900)
                     /gene="chaA"
                     /locus_tag="N7B39_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HGU2422892.1"
                     /translation="VKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINLLALIGIL
                     SSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTLMRDTLYS
                     IIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMALPAANFS
                     TGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAHSSLWHAI
                     WLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGALKAVLNNQ
                     VQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLVLCHISFS
                     TGRTNVLNGAAHLALFAAYLMTIFA"
BASE COUNT         3742 a         3776 c         3844 g         3538 t
ORIGIN      
        1 gggactttat ctgcgggata ttacccttca ggtaagtcct tttctcgcca gcatattgga
       61 gcgtggtcaa tattgatcgg ctgtgaaggg ggctgatgtg tagtggaaca aaaactgacg
      121 ttatatcact gctaatgaga tatccgtcat ctgaacactg atgtctttaa ttattctcgt
      181 ggttcactgt aggcaataag cacaaaagtg taggatgtta caagaatgat taggactcgg
      241 tgaaatgaaa aatccacgca attgcgtgga ttttatatac ttttgcgctc ttcatgagat
      301 ttagcgaaac ctcatgagac aataaattaa ttagacgttg aaaaggaagt tcatcacatc
      361 gccatctttc acgatgtaat ctttaccttc tgcacgcatt ttgcctgctt ctttcgcgcc
      421 ttgttcacct ttgtaagtga tgaaatcttc aaacgagatg gtttgtgcac ggataaagcc
      481 tttttcaaaa tcagtatgga ttttgcccgc tgcctgcggc gcggttgctc caaccggaat
      541 ggtccatgca cgcacttctt tcaccccagc ggtgaagtaa gtttgcaggt tcagcagctt
      601 ataaccggca cggatcacac ggttcagacc cggctcttcc agcccaagct cctgcataaa
      661 ctcgtcacgt tcttcgtcgt ccagttcggc aatgtctgct tcaacagcag cacaaaccgg
      721 aaccacaaca gaaccttctt tcgccgcgat ttcacgcacc tggtcaagat atgggttgtt
      781 ttcaaaaccg tcttcgttga cgttggcgat gtacattgtt ggtttcagcg tcaggaagct
      841 caggtaacga atagccgctt tctcttcagc gcttaaatcc agcgcgcgca gcatacctgc
      901 gttttccagc tggggcaggc atttttccag gaccgccagc tcagctttcg cgtctttatc
      961 gccacctttg gctttcttct gtacgcgatg aatcgcacgt tcgcaggtgt cgaggtctgc
     1021 cagcgccagt tcggtgttga taacttcaat atcgtcagcc gggttaactt tgccggaaac
     1081 gtgaatgatg ttgtcatttt caaagcagcg aacaacgtga ccgatcgctt cggtttcacg
     1141 gatgttggtc aggaactggt tacccagacc ttcgcctttc gatgcgcctt ttaccagacc
     1201 ggcgatatcg acaaattcca tggtcgtggg aagcgtacgt tgcggtttta cgatttcagc
     1261 cagttgatcc aggcgaggat caggcattgg tacgacgcct gtgttcggct caatggtgca
     1321 gaatggaaag ttggccgctt caataccggc tttggtcagc gcgttgaaca gggtagattt
     1381 cccgacgttg ggcaaaccga cgataccgca tttgaatccc atgatttaaa tcaccttaat
     1441 atcttaataa tcaacctgtt attgctaaca gattgcagaa atggaaataa ctttgcctat
     1501 tatacacggc actcggcaaa aatgccgcag acaacgactt attgcgcttt aaaggcgtgc
     1561 aatcggttcg ttgctttggt caagccatct gtaaaccaca tttcagtaca acgcgccgct
     1621 tcgtcgatgg cttcatcaat taacttctgt tcactaacag gcggtttgcc taacacaaaa
     1681 ccaacaactt tgtttttatc gcccggatga ccgattccga tgcgtaaacg gtgaaagtta
     1741 gggttattac ccaatttact gatgatgtct ttcagtccat tgtgaccacc atggccaccg
     1801 cccaacttaa atttggcgac gccaggaggc agatccagtt cgtcgtgggc caccagaatt
     1861 tcgtccggat taatgcggaa aaaactggcc atcgccgcaa cggctttgcc gctgagattc
     1921 ataaatgtag tcgggactaa caggcggaca tcttcgcctc caagggtgac tcgcgaagta
     1981 taaccaaaga atttagcctc ttcgcgcagc ggagcgcgca aacgctctgc cagtaagtca
     2041 acgaaccagg caccagcatt atgtcgcgtt gcggcgtatt cagcaccggg gttcgccagg
     2101 ccgacaatca atttaatcgt cacgtttttt tgtcctgagt gtgtacataa ctggcgcgta
     2161 gtttactggt tgcggccccg cttgacaaaa aactgcgtat caaatgcaga taacgtaata
     2221 attgcctgag tggactatta gaaagtcaag gtgttcaggc gtttatttgt aaagttttgt
     2281 tgaaataagg gttgtaattg tgatcacgcc cgcacataac ccacagggtg ttgtctatac
     2341 tttacacata aggaagaggg gtattccctg ttacaaccca gaaagttccg gaggtgacat
     2401 atgaaacgca aaaacgcttc gttactcggt aacgtgctca tggggttggg tctggtggta
     2461 atggtggtcg gcgtggggta ttcaatcctc aaccagttac cacagtttaa tatgccccag
     2521 tatttcgcac atggtgcagt gctaagtatt ttcgtcggtg ccattctctg gctggcgggt
     2581 gcccgtgttg gcgggcatga acaggtgtgc gaccgttact ggtgggttcg ccactatgac
     2641 aaacgttgcc gccgtagcga taatcgccgt catagctaac aaataatgca gtttgctgac
     2701 cagtcagttt gcgctgactg gtcaatctcg tacttataaa tccgccatcg ccgcgcgacg
     2761 attcgggaag aacgcgaggc gtcccgggat cggttgaatg cctgcgcgag ccatagtgcg
     2821 cagtggctgg aattccacgt tgcacacgcg cagttcacat ccttcgggca gacgcttcac
     2881 aaaacgctgg aacgcatcaa gaccaccagc atcaagtacc ggaacggcat cccacttcag
     2941 aatcacaatc cgtttgcctt caagacgtga ctccaggtcc gtgaataagc cttcagcagc
     3001 agcaaaaaac agcgggccaa taacgcgcag aaccaggaca tcgtctggaa catctacgac
     3061 taccggtgcc aggcgagtca tacgtgcgat acgacgcata aacagcagcg atgccagcac
     3121 gatccccacg ctgatggcaa taaccatatc aaacagcacg gtcagcgaca tgcacagcag
     3181 catgacaatg atgtcatctt tcggtgcatg acgcagcaag tcgaccactt tatgcgcttc
     3241 actcatgttc cacgccacca tcaacagcag ggctgccata gcggaaagcg gcagccagga
     3301 gagcagcggt gccagtacca gcagggcaag aataaccaga atagagtgga tcaccgccga
     3361 gataggggaa gttgccccgg cacggacgtt agcggcagaa cgcgcgatgg cagcggtagc
     3421 ggtaatacca ccaaagaacg gagcgatgat attccccagc ccctgtccaa ccagttcgct
     3481 attcgccttg tgcttcgtcc cggtcatacc atccagcacc acggcgcaga gcagagattc
     3541 gattgcgccg agcattgcca ttgagaatgc cgcaggcagc agtgtgcgaa tagaatccca
     3601 ggttagcgtg aattctgaat taggcagatc ccacggcagc accagttgcg gtagcagttg
     3661 cggaataccg ttaccctgag aaccatcggc caggacgtag tggaattgcg aaccgatggt
     3721 agcaacatgt ccgccgagca ggttaacaat ccccatcacc gcgcaaccag ccagcaaggc
     3781 cggaaggtga ccgggtaaac gaatgcccag acgcggccag aaaacaagaa tacctagcgt
     3841 cacaatgcca atggcagcat cacccacatt aatggtcggc agcgccataa ataatgcgcc
     3901 gactttttgt agataatgtt ccgggacatg ggccatttgc agaccgagaa aatctttaat
     3961 ctgcatggta ccgatggtga tcccgatacc cgaggtgaaa cctaaggtga cggaaaccgg
     4021 aatatactca atcaggcgac caaagcgtgc cagacccatc agaatcaaaa agatccccga
     4081 cagcaaggtc gcaaccagca gtcctgccag cccaaactgt tgcgaaacgg gatagagaat
     4141 taccacaaat gccgcagtcg gaccggaaac gctaaagcgt gacccacccg tcagagcaat
     4201 gacaatcccc gcaacagctg cggtatataa accgtactgg ggtgccacac cactaccaat
     4261 agccaacgcc atcgccagcg ggatagcaat aatcccgacg gttatcccgg caatcaggtc
     4321 acgggtaaac cgtgcggcag tatatttttc tttccagcaa gcgtcgatca gagcgcggaa
     4381 aggcatcaca tgtgaggaaa atattttgtt cacaataatg tttcatccgt gagcgcatca
     4441 tctgtcaact aaatggcagg tgaaggaggc ataggtcata caaatggata ttacagacaa
     4501 aaaaacccgc cgcagcgggt ctttgagccg ggttcgatta gtgttcgaac atggcagaga
     4561 tcgattcttc gttgctgata cgacgaatcg cttcggccag catacctgac agggtcagag
     4621 tacgcacgtt cggcagtgat ttgatttcat cgctcagcgg aatggtatcg cagacaacga
     4681 cttcatcaat tacagagtta cgcaggttgt tcgccgcgtt gccagagaag atcgggtgag
     4741 tcgcgtacgc aaatacacgt ttagcaccac gttctttcag agcttcagca gctttacaca
     4801 gcgtaccgcc agtgtcgatc atatcatcga ccagtacgca gtcacgacct gcaacgtcac
     4861 cgatgatatg catcacctgg gaaacgttcg cacgcggacg acgtttgtcg atgattgcca
     4921 tatcggtatc gttcagcagc ttagcgatag cgcgggcacg cacaacgccg ccgatgtccg
     4981 gagaaaccac aattgggtta tccagattca gctgcagcat gtcttccagc aggatcgggc
     5041 taccaaatac gttatcaacc ggaacgtcga agaaaccctg aatctgttca gcgtgcagat
     5101 ccactgtcag cacacggtca acaccgacgc tggagaggaa gtctgcaacc actttcgcag
     5161 tgattggtac acgagcggaa cggacgcgac ggtcctggcg cgcatagcca aagtagggga
     5221 taacagcggt gatacgacct gcggaagcac gacgcagggc atcaaccata acgactaatt
     5281 ccatcaggtt gtcgttagta ggggcacaag tggactggat gatgaaaata tcaccaccgc
     5341 gtacattttc attaatttgt acgctgactt cgccatcgct aaagcgacct acagcggcgt
     5401 cgccgagtga agtgtacagg cggttggcaa tacgttgtgc tagttccggg gtggcgttac
     5461 cagcaaaaag cttcatatca ggcacgagaa gaacctcagg catgcgtcca ttggtggaaa
     5521 gaatctgccg aaaactgtgc gggccaggca tgatcctttc caggcggtgt attaaagagc
     5581 gcgatgcaac gtctggaaca aggtgacgtt gtcaccgaaa ctcagcttgc ccggcttaaa
     5641 gcatggctct gtgcaatggg gaaagattag cgcctttcgc cacaaagcca ttgagccatt
     5701 ccggggcttg ctctagcacc tggcgggctt cagactctgt atcaaattca gcaaagacac
     5761 aggcccctgt cccagtcagg cgcgacgggg cgtattctaa cagccaggaa agcaccgcat
     5821 caacctcgcg aaaacgtttt cttgcgataa cctcgcaatc attgctgaat tcacatttta
     5881 gcaacgtttc tattgacctt tttggcgtat tgcgcgggag ttcaggatct ttaaaaatca
     5941 ccggagtcgg aatacttaca ccagggtgcg ccaccagata ccacttctct ggcggatcca
     6001 ccggggttag tatttcacca acgccttcgg caaacgcggc atgcccccga acaaagacag
     6061 gaacatctgc gcccagcgtc agccccattt ccgccagctc atccatgctt agcccgcatt
     6121 gccagagatg atttaatgcc accaggaccg tcgcggcatt ggatgaacca ccgccgagac
     6181 cgccgcccat cggcaaacgc ttgtcaatgc tgatattcgc accgcttccc gtcggaagac
     6241 gcccgctgtc tgccgcagtt ttcatcaaca accgcgctgc gcgaacgatc aggttatctt
     6301 catgttccac gccttcaacg ggcgttaaca gacggatatc cccatcgtca cgaagctcaa
     6361 tgctgatggt gtcgccgtaa tcaagaaact gaaacagcgt ttgcagcgtg tggtaaccat
     6421 ccgcacgctg accggtaatg tataaaaaca gattaagttt tgccggagag ggccactgtg
     6481 tccgcattat ttcactatcc agttatccat ttttaacttg atgcgttgac caccgtcggt
     6541 gagttccata ttggctggca tcgcaggttg cgttttggtg tcataaccac cataaacaac
     6601 cttccagttt ttgccattct ggctgtaggt aatttcgctc aggcggtact ggtcgtccag
     6661 tttgtagtcg gttgcatcac ccggtaaacc taaaatccac tggcgcaagc tgttgagcgg
     6721 aattggcatt ccagtcaatt tgccaatcat ctcttcggcg tcatcggcgg tataacgctg
     6781 acctttattg tcgactaact gcacgttacc cggttgagca ttcagctcca gttccgtgct
     6841 gcccaatggg ttagtgagca gcagacggta gcgatcctgg ccggtttgct gccagaaaaa
     6901 gcgggcgtac actttttgtt ggtcagaaat ataagcgaac gcgccgcgag tctgatactg
     6961 attaagattg cgcacgtctt gctgatgctg acgccattgt ggcgaatccg ggcttttgcc
     7021 aggacctttg ggcgtggtaa cggaacaggc agtgagcaca agagcagcca gcggtagcag
     7081 gcggataaga cgaaaatcgg gcaggggcat agtgatgaca agtccttgag atacgttgca
     7141 gttataaccc ttaatgctag cgtcaccatt agtcatcgtc tatgttcaag ttgtcttaat
     7201 tgtagaaatc taacgggttc gggcaattac tccaaaaggg ggcgctccct tttattgatc
     7261 tcgcgcatac ggtatgatgc gccgagacta accttatcaa cgttggtatt atttcccgca
     7321 gacatgaccc ttttagcgct cggtattaac cataaaacgg cacctgtatc gctgcgagaa
     7381 cgtgtatcgt tttcgccgga caaacttgat caggcgctgg acagtctgct cgcacagccg
     7441 atggtgcagg gcggggtggt gctgtcgacg tgtaaccgca ctgagcttta tcttagcgtc
     7501 gaagagcagg acaacttgca agaggcgtta atccgctggc tttgcgatta tcacaatctt
     7561 aatgaagaag atctgcgtaa aagcctctac tggcatcagg ataacgacgc ggttagccat
     7621 ttaatgcgtg ttgccagcgg cctggattca ctggttcttg gggagccgca gatcctcggt
     7681 caggttaaaa aagcgtttgc cgattcgcaa aaaggtcata tgaaggccag cgaactggaa
     7741 cgcatgttcc agaaatcttt ctctgtagcg aaacgcgtac gcactgaaac agatatcggt
     7801 gccagcgctg tgtctgtcgc ttttgcggct tgtacgctgg cgcggcagat ctttgaatcg
     7861 ctctctacgg tcacagtgtt gctggtaggc gcgggcgaaa ccatcgagct ggtagcgcgt
     7921 catctgcgcg aacataaagt acagaagatg attatcgcca accgcactcg cgaacgtgcc
     7981 caaattctgg cagatgaagt cggcgcggaa gtgattgccc tgagtgatat cgacgaacgt
     8041 ctgcgcgaag ccgatatcat catcagttcc accgccagcc cgttaccgat tatcgggaaa
     8101 ggcatggtgg agcgcgcatt aaaaagccgt cgcaaccaac caatgctgtt ggtggatatt
     8161 gccgttccgc gcgatgttga gccggaagtt ggcaaactgg cgaatgctta tctttatagc
     8221 gttgatgatc tgcaaagcat catttcgcac aacctggcgc agcgtaaagc cgcagcggtt
     8281 gaggcggaaa ctattgtcgc tcaggaaacc agcgaattta tggcgtggct gcgagcacaa
     8341 agcgccagcg aaaccattcg cgagtatcgc agccaggcag agcaagttcg cgatgagtta
     8401 accgccaaag cgttagcggc ccttgagcag ggcggcgacg cgcaagccat tatgcaggat
     8461 ctggcatgga aactgactaa ccgcttgatc catgcgccaa cgaaatcact tcaacaggcc
     8521 gcccgtgacg gggataacga acgcctgaat attctgcgcg acagcctcgg gctggagtag
     8581 cagtacatca ttttcttttt ttacagggtg catttacgcc tatgaagcct tctatcgttg
     8641 ccaaactgga agccctgcat gaacgccatg aagaagttca ggcgttgctg ggtgacgcgc
     8701 aaactatcgc cgaccaggaa cgttttcgcg cattatcacg cgaatatgcg cagttaagtg
     8761 atgtttcgcg ctgttttacc gactggcaac aggttcagga agatatcgaa accgcacaga
     8821 tgatgctcga tgatcctgaa atgcgtgaga tggcgcagga tgaactgcgc gaagctaaag
     8881 aaaaaagcga gcaactggaa cagcaattac aggttctgtt actgccaaaa gatcctgatg
     8941 acgaacgtaa cgccttcctc gaagtccgag ccggaaccgg cggcgacgaa gcagcgctgt
     9001 tcgcaggcga tctgttccgt atgtacagcc gttacgccga agcccgccgc tggcgggtag
     9061 aaatcatgag cgccagcgag ggtgaacatg gtggttataa agagatcatc gccaaaatta
     9121 gcggtgatgg tgtgtatggt cgtctgaaat ttgaatctgg cggtcatcgc gtgcagcgtg
     9181 ttcctgctac ggaatcgcag ggtcgtattc atacttctgc ttgtaccgtt gcggtaatgc
     9241 cagaactgcc tgacgcagaa ctgccggaca tcaacccagc agatttacgc attgatactt
     9301 tccgctcgtc aggggcgggt ggtcagcacg ttaacaccac cgattcggca attcgtatta
     9361 ctcacttgcc gaccgggatt gttgttgaat gtcaggacga acgttcacaa cataaaaaca
     9421 aagctaaagc actttctgta ctcggtgctc gcatccacgc tgctgaaatg gcaaaacggc
     9481 aacaggccga agcgtctacc cgtcgtaacc tgctggggag tggcgatcgc agcgaccgta
     9541 accgtactta caacttcccg caggggcgcg ttaccgatca ccgcatcaac ctgacgctct
     9601 accgcctgga tgaagtgatg gaaggtaagc tggatatgct gattgaaccg attatccagg
     9661 aacatcaggc cgaccaactg gcggcgttgt ccgagcagga ataatggaat atcaacactg
     9721 gttacgtgaa gcaataagcc aacttcaggc gagcgaaagc ccgcggcgtg atgctgaaat
     9781 cctgctggag catgttaccg gcagagggcg tacttttatt ctcgcctttg gtgaaacgca
     9841 gctgactgac gaacaatgtc agcaacttga tgcgctactg acacgtcgtc gcgatggtga
     9901 acccattgct catttaaccg gggtgcgaga attctggtcg ttgccgttat ttgtttcgcc
     9961 agcgacctta attccgcgcc cggatacgga gtgtctggtg gagcaggcac tggcgcggtt
    10021 gcctgaacaa ccttgccgta ttctcgatct cgggacgggt accggggcga ttgcgcttgc
    10081 gctggctcgc gagcgcccgg actgcgaaat taccgctgta gatcgtatgc ccgatgctgt
    10141 cgccctggca caacgtaatg cccagcatct ggcgatcaaa aatatccaca ttctgcaaag
    10201 cgactggttt agcgcgctag ccgggcagca gtttgcgatg attgtcagca atccgccgta
    10261 tattgacgag caggaccctc atcttcaaca aggcgatgtc cgctttgagc cgctcactgc
    10321 gctggttgcg gcagacagtg gaatggcaga catcgtgcat atcatcgaac agtcgcgtaa
    10381 cgcgctggta tccggcggct ttctgcttct ggaacatggc tggcagcagg gcgaagcggt
    10441 gcgacaggca tttatcctcg cggggtatca tgacgtcgaa acctgccgtg actatggtga
    10501 taacgagcgc gtaacgctcg gccgctatta tcaatgacaa gtttttctac actgcttagt
    10561 gttcatctta ttagtatcgc gctttctgtt gggctattaa ccttacgttt ctggctacgt
    10621 tatcagaagc atccacaggc atttgctcgc tggacgcgca ttgtgccgcc ggttgtcgat
    10681 acggtgttat tgttaagcgg cattgcgttg atggctaaag cgcacatcct gccattttcc
    10741 gggcaggcac agtggctgac tgaaaagctg tttggagtta tcatttatat cgttttgggt
    10801 tttattgcac tcgattatcg tcgtatgcac agtcagcagg cgcgcattat tgccttcccg
    10861 ctgacgttgg tggtgctgta catcatcatt aaactcgcca ccacaaaagt accgttactg
    10921 gggtaagtca tgagatcgtt agctgatttc gaatttaata aagcgccatt gtgcgaaggc
    10981 atgatcctgg cttgcgaagc aatccgccgc gattttccct cgcaagatgt ttacgacgaa
    11041 ctggagcgtc tcgttagtct ggcgaaggaa gaaatcagcc agcttctgcc tttagaagag
    11101 cagctggaaa aactgatcgc gctgttttac ggcgactggg gatttaaagc ctcacgcggc
    11161 gtttatcgtc tttccgatgc attatggctg gaccaggtgt taaagaatcg acagggcagt
    11221 gcggtatcat taggtgcggt tttattatgg gtcgcgaatc gtctcgattt gccgctgctg
    11281 ccggtgattt tccctacgca gctgatattg cgcattgaat gtccggatgg cgaaatttgg
    11341 ctgattaatc cttttaacgg tgaatcgtta agcgaacata tgctggacgt atggttaaag
    11401 ggaaatatca gcccgtcggc ggaactgttt tatgaagacc ttgatgaagc tgataacatt
    11461 gaggtaatcc gcaaattgct ggatacactc aaagcctcgt tgatggaaga aaatcagatg
    11521 gagctggcgt tacgcaccag cgaagcttta ttacaattca acccggaaga tccctatgaa
    11581 attcgcgatc gcgggttgat ttatgcgcaa ctggattgcg aacacgttgc gttgaacgat
    11641 ttaagttatt tcgttgaaca gtgtccggaa gacccgatca gcgaaatgat ccgtgcgcaa
    11701 ataaataaca tcgcgcataa acatattgtg ctgcattaat taatcgacat tttactcaag
    11761 gttaaggcga tcctatgaaa caaaaagtgg ttagcattgg cgacatcaac gtagcaaatg
    11821 acctgccgtt cgtactgttt ggcggtatga acgtgctgga atctcgcgat ctggcgatgc
    11881 gcatttgcga gcactacgta accgtgaccc agaaactggg tatcccttac gtgttcaaag
    11941 cctcttttga caaagccaac cgctcctcca tccactctta tcgtggaccg ggcctggaag
    12001 aagggatgaa aatcttccag gagttgaagc agacttttgg cgtgaaaatt atcaccgacg
    12061 ttcacgaacc aagtcaggca cagcccgttg ctgatgtcgt ggatgtgatt cagttgccgg
    12121 cgtttcttgc tcgccagact gacctggttg aagccatggc gaaaaccggt gcggtaatta
    12181 acgtcaagaa accacagttt gtcagcccgg gacagatggg taatatcgtt gataaattca
    12241 aagaaggcgg caacgaaaaa gtgattcttt gcgatcgtgg tgctaacttc ggctatgaca
    12301 acctggttgt cgatatgctg ggcttcagca ttatgaagaa agtgtctggt aactcgccgg
    12361 tgattttcga cgtgacccac gcattgcaat gccgcgatcc ttttggcgca gcttccggcg
    12421 gtcgtcgtgc tcaggtggct gagctggcac gagccggtat ggcggtaggt ctggcggggc
    12481 tgtttattga agcgcatccg gatccggaac atgcgaaatg tgatggtcca tccgcgctgc
    12541 cgctggctaa actggaaccg ttcctcaggc agatgaaagc gattgatgat ctggtgaaag
    12601 gtttcgaaga actggatacc agcaagtaat cttttttgct tgaaaaataa agtcttagcg
    12661 ttctgcgtta agactttttt catgggtgcc ggatacaaaa aaggccgcag gctgttaccc
    12721 tgcggccggt ttcgggcgca tattgccatc acggcagcct gacgcccgtt ttcgccttac
    12781 ttccggttac gccaccagcc gacaatcgct gcggtaataa ttcccgccag gatcggtgct
    12841 gccaggtcgt gccagaaaat catggcaaac tgcgcgagcg tcatatagcc gccttgttgt
    12901 aatgacaaca ttttgcggct attcttgaat tgttgatggt tcaagattag cccccgttct
    12961 gttgtcaggt tttacctctc aacgtgcggg ggttttctct ttccagcaac caatgccacc
    13021 agggataaag cccccgcaac attgcgcctc accggataac gccggcttgg tgtggatact
    13081 acgactcaat tcatcttcac ttcatccctg aaatgtttgc aatgaagagt gcattccggt
    13141 ttttcaacag ctgttacagt cgtttcatga gtgctctgga tgatgcttcc agctcgggtt
    13201 gccaatattt acttgtggaa gtgataaaga caaaaatggc cgcaggctgt tacccctgcg
    13261 gccggtttcg ggcgcatatt gccatcacgg cagcctgacg cccgttttca ccttacttcc
    13321 ggttacgcca ccagccgaca atcgctgcgg taataattcc cgccaggatc ggtgccgcca
    13381 ggtcgtgcca gaaagtcatg gcaaactgcg cgagcgtcat atagccgcct tgttgtaatg
    13441 acaacatttt gcggctattc ttgaagtgtc tggtttcaag attagccctg ttgtcaggtt
    13501 ttacctctca acgtgcgggg gttttctctt tccagcaacc aatgccacca gggataaaac
    13561 ccccgcaaca ttgcgcctca ccggataacg ccggcttggt gtggatacta cgtctcaatt
    13621 catcttcact tcatccctga aatgtttgca ataaagagta cattccggct tttcaacagc
    13681 tgttgcagtg gtttcgtgca ggttgtggta caggctcgca attctgatta acgacctgtc
    13741 caggtagtat gaagaaatac aataagcagg aatttatatt atctcccccg gtttccgtca
    13801 tcggaggaga tataactgtc aggcaaatat cgtcatcaaa taggcggcaa acagtgccag
    13861 atgcgctgcg ccattgagca cgttagtacg tccggtggag aaggagatat ggcacagcac
    13921 taaagaggcc accatcacca ccatttctgg cgcaccaagt gcaaactgca attcgttacc
    13981 cgtcataaag gcaattagcg tgacgacagg tacggtaagc gaaattgttg ctaacactga
    14041 accaaagaac agattcatcg cgcgctggac ctggttgttc aacactgctt ttaatgcacc
    14101 taaaccttcc ggcgacagaa tcaacagtgc caccaggaag ccagtaaagg cgacaggggc
    14161 attcatgctg tcgagcaaag tctccagcgg gctggcgttc attttggtga ctgcaataac
    14221 ggcaatcagg tggataatca accagatagc atgccacagg ctgctatggg cagacggttt
    14281 accgtggtgc gggtcgtcgt catcactgtc atcttcgtgc tcgtagacaa acaaactttg
    14341 atgcgttttg gtctggatca gcaaaaatac accatacatt gccgcagaaa ttaatgctac
    14401 cagtaacgcc tgaccggttg aaaaattcgc cgcaggcaga gccattggaa ataccagtac
    14461 gattatcgcc agggggaaca gcgcaattaa atactgctta ataccaaaca gattcatata
    14521 ttgggtggca aacttacgac cgcccaacaa taatgaaaag ccgaccagcc caccggtaac
    14581 aatcataatg atcgaataga gcgtatcacg cattagcgtt ggcgcggcgt cgccggttgc
    14641 cattaaagct gaaatcaaac taacttcaag aataaccact gaaaggctaa gaataagcga
    14701 accgtaaggt tctcccaggc gatgggctaa tacgtccgca tgacggacaa cactaaaggc
    14761 gctacttaaa ataccaataa gcgcaagaag attgatggca atgaccactg gtagtgtctg
    14821 gctgcttccc cacaggaaca gcactaccag cgccagaacc gggaaaataa gcgaagtctc
    14881 cttgtggcgg gtttttaccg
//
LOCUS       DBFIFI010000101        14785 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.101, whole genome shotgun sequence.
ACCESSION   DBFIFI010000101 DBFIFI010000000
VERSION     DBFIFI010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14785
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.101"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            116..358
                     /gene="yjbE"
                     /locus_tag="N7B39_002941"
     CDS             116..358
                     /gene="yjbE"
                     /locus_tag="N7B39_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HGU2422893.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            472..1110
                     /locus_tag="N7B39_002942"
     CDS             472..1110
                     /locus_tag="N7B39_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HGU2422894.1"
                     /translation="MKRPAFILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIIDGATWMRTMGWTEYQQVRYATARSVFKWDGTGTVKVGSDET
                     AVRVLDEEVSTDQARWHNRYWIDSGGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1107..1844
                     /locus_tag="N7B39_002943"
     CDS             1107..1844
                     /locus_tag="N7B39_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HGU2422895.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERANPPLQGNYTLWVGPPPSTVTLFGLISRPGNQP
                     FTPGRDVASYLSGQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSEMPDALNADILQTLTQRIPQ"
     gene            1844..3940
                     /locus_tag="N7B39_002944"
     CDS             1844..3940
                     /locus_tag="N7B39_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HGU2422896.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERANTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIVRANRIVMNDLPDGIKTIRVTENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLAQKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(3987..4265)
                     /locus_tag="N7B39_002945"
     CDS             complement(3987..4265)
                     /locus_tag="N7B39_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2422897.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSALPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4266..4409
                     /locus_tag="N7B39_002946"
     CDS             4266..4409
                     /locus_tag="N7B39_002946"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2422898.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            4535..4945
                     /gene="psiE"
                     /locus_tag="N7B39_002947"
     CDS             4535..4945
                     /gene="psiE"
                     /locus_tag="N7B39_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HGU2422899.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQASKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVVTLWLCNSKRLKRE"
     gene            complement(4989..6464)
                     /gene="xylE"
                     /locus_tag="N7B39_002948"
     CDS             complement(4989..6464)
                     /gene="xylE"
                     /locus_tag="N7B39_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HGU2422900.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPVYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAEGILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(6836..7726)
                     /gene="malG"
                     /locus_tag="N7B39_002949"
     CDS             complement(6836..7726)
                     /gene="malG"
                     /locus_tag="N7B39_002949"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HGU2422901.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(7741..9285)
                     /gene="malF"
                     /locus_tag="N7B39_002950"
     CDS             complement(7741..9285)
                     /gene="malF"
                     /locus_tag="N7B39_002950"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HGU2422902.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGREQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(9439..10629)
                     /gene="malE"
                     /locus_tag="N7B39_002951"
     CDS             complement(9439..10629)
                     /gene="malE"
                     /locus_tag="N7B39_002951"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HGU2422903.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            10994..12109
                     /gene="malK"
                     /locus_tag="N7B39_002952"
     CDS             10994..12109
                     /gene="malK"
                     /locus_tag="N7B39_002952"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HGU2422904.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            12181..13521
                     /gene="lamB"
                     /locus_tag="N7B39_002953"
     CDS             12181..13521
                     /gene="lamB"
                     /locus_tag="N7B39_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HGU2422905.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEVNPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            13672..14592
                     /gene="malM"
                     /locus_tag="N7B39_002954"
     CDS             13672..14592
                     /gene="malM"
                     /locus_tag="N7B39_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HGU2422906.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
BASE COUNT         3640 a         3819 c         3910 g         3416 t
ORIGIN      
        1 aaaaccccat ctctaattcc ccgcgccgta attcgcatgc tttagttgtg tatactcgat
       61 cccgcccgaa atgtttttgg gtaaatctcc attcattcaa tgaagggaaa ttgttatgaa
      121 aaaagttctg tatggcattt ttgccatatc tgcgcttgcg gcgacttctg cgtgggctgc
      181 acctgtacag gtgggcgaag cggcagggtc ggcagcaacg tcggtttcgg cggggagttc
      241 ctccgcgacc agcgtcagca ccgtaagctc ggcggtgggt gtcgcgctcg cggcaaccgg
      301 tggcggtgat ggttctaata ccgggaccac cacaaccacg accaccagta cccagtaata
      361 aagtatgtat ccccaaaata attcgagtca ttgcatctgt ggctagaagt atgaagggat
      421 ttaccataac cacactccgg tgtggttatt ctgcccctct ggagaagagt cgtgaagcga
      481 cctgcattca ttcttatctg cctgctatta caggcctgtt cagccacgac taaagagctg
      541 ggcaattcac tgtgggacag tctgttcggc acgccaggcg tacagctgac ggacgatgat
      601 attcaaaata tgccctacgc cagccagtac atgcagctta atggtgggcc gcagttgttt
      661 gtggtgctgg ccttcgctga agacggacaa caaaaatggg tcactcagga tcaggccact
      721 ctcgtcacac aacatggtcg tctggtgaag actttgcttg gcggcgacaa cctgattgaa
      781 gtgaataacc ttgccgccga cccgctgatt aaacccgcac aaatcattga tggcgcaacc
      841 tggatgcgca cgatgggctg gaccgagtac cagcaggtac gctacgccac cgcacgctca
      901 gtcttcaaat gggatggcac cggtaccgtt aaggtcggca gcgatgaaac cgcggttcgc
      961 gtgctggacg aagaagtctc caccgaccag gcgcgctggc ataaccgcta ttggatcgac
     1021 agcggagggc aaattcgcca gtcggaacag tatctcggcg cggattattt cccggtgaaa
     1081 accactctca tcaaggcggc aaaacaatga ttaaacaaac tattgtcgcg ttgcttttga
     1141 gtgtgggagc gtcatcggtc ttcgcggcag gaaccgtcaa ggtgttcagc aatggcagca
     1201 gtgaggccaa aacgctaacg ggcgcagagc atttaatcga tctggtaggc caaccgcggc
     1261 tggcaaacag ctggtggcct ggtgcggtga ttagcgaaga gctggcaacg gcggcagcat
     1321 tgcgtcagca gcaggcgttg ctgacacggc tggcagaaca gggcgcagat tccagcgccg
     1381 acgatgccgc tgcgattaac gccttacgcc agcaaattca ggcgttgaag gtgacgggca
     1441 ggcaaaaaat caatcttgat cccgatatcg tccgcgttgc tgaacgcgct aacccgccgt
     1501 tgcagggcaa ctacacgctg tgggtcggac caccaccgtc cacggtcacg ttgttcgggc
     1561 ttatcagccg tcctggcaat cagccattca ctcccggtcg cgacgtggcg agctatctct
     1621 ccgggcaaag tctgcttagc ggtgcggatc gtagctacgc gtgggtggtt tacccggacg
     1681 gacgcacaca aaaagcgccg gtggcttact ggaacaagcg tcacgtagag ccgatgcccg
     1741 gcagcattat ttatgttggc ctcgcggact ccgtctggag tgagatgcct gatgccctta
     1801 acgccgacat tcttcagact ctgacgcagc ggatacctca ataatgaaaa aaagacatct
     1861 gcttagctta ctggcgctgg gcattagcac agcttgctac ggcgaaacat atcctgcgcc
     1921 cattggtccg tcgcagtcgg atttcggtgg cgtaggatta ttacaaacgc ccacggctcg
     1981 catggcgcgg gaaggggagt tgagtctgaa ctatcgcgat aacgatcagt accgttatta
     2041 ctcagcttca gtgcaactct tcccgtggct ggaaacaacg ctgcgctaca ccgacgtgcg
     2101 cacccggcag tacagcagcg tcgaagcgtt ctctggcgat caaacgtata aagataaagc
     2161 cttcgatctc aaactgcgtt tgtgggaaga gagttactgg ctgccgcaag tggcggttgg
     2221 tgcgcgggat atcggcggta cggggctgtt tgatgcggaa tatcttgttg ccagcaaagc
     2281 ctgggggccg ttcgatttta cgcttggcct gggctgggga tatttgggca caagcggtaa
     2341 tgtgaaaaat ccgctctgtt cagccagtga taaatattgc tatcgcgata acagctacaa
     2401 acaggcggga tctatcgacg gtagccagat gttccacggt cctgcctcac tgtttggcgg
     2461 cgtggaatac cagacgccct ggcaaccgct gcgcctgaaa ctggagtatg aaggcaataa
     2521 ttatcagcag gattttgccg gtaagctgga gcaaaaaagt aagtttaacg tcggggcgat
     2581 ttatcgcgtt accgattggg ccgacgttaa ccttagctat gaacgtgcca acacttttat
     2641 gtttggcgtc acgctgcgca ccaactttaa cgatctgcgc ccgtcttaca acgataacgc
     2701 ccgcccgcaa tatcaaccgc agccgcagga tgccattttg cagcattcgg tggtggcgaa
     2761 tcagttaacg ctgttgaaat acaatgccgg acttgccgat ccgcagatcc aggcgaaagg
     2821 cgatacgctg tatgttaccg gcgagcaggt gaaatatcgt gattcgcgcg aagggatcgt
     2881 tcgtgctaat cggatcgtga tgaatgatct gccggatggg atcaagacga tccgcgttac
     2941 ggaaaatcgc cttaacatgc cgcaggtgac gacggaaacg gatgtcgcca gcctgaaaaa
     3001 tcatctcgcc ggagagccgt tgggccacga aacgaagctg gcgcaaaaac gcgtcgagcc
     3061 agtggttccg aagtccaccg agcagggctg gtatatcgac aaatcgcgct ttgatttcca
     3121 tatcgatccg gtgctgaacc agtcggtcgg tggcccggaa aacttttaca tgtatcagct
     3181 gggcgtgatg ggaacggcag atttgtggct gacggaccat ctgctgacca ccggcagcct
     3241 gtttgcaaat cttgccaaca actacgacaa gtttaactac accaatccgc cgcaggactc
     3301 gcacttaccg cgcgtgcgta cccatgtgcg cgagtatgtg cagaacgatg tctatgtgaa
     3361 taacctgcaa gccaactact tccagcatct gggcaacggc ttctacggtc aggtctacgg
     3421 aggttatctc gaaaccatgt ttggcggtgc gggggcagaa gtgttgtatc gcccgctgga
     3481 cagcaactgg gcgtttggtc tggatgccaa ctacgttaaa cagcgcgact ggcgtagtgc
     3541 aaaggatatg atgaaattca ccgactacag cgtgaaaacc ggacatctga ccgcctactg
     3601 gacgccatct ttcgctcagg atgtgttagt taaagccagc gtcgggcagt atctggcagg
     3661 ggataaaggc ggcacgctgg agatcgctaa acgctttgac agcggcgtgg tggtgggcgg
     3721 ctatgccacg atcaccaacg tttcgaaaga ggagtacggc gaaggggact tcaccaaagg
     3781 cgtgtatgtc tctgtaccgt tggatctctt ctcgtctggc ccgacacgca gccgtgcggc
     3841 gattggctgg acgccgctga cgcgtgacgg tggtcagcaa cttgggcgta agttccagtt
     3901 gtatgacatg accagcgacc gtagcgtcaa tttccgctaa gtcatgggaa aggtgccagt
     3961 tttcgcaatc actggcacct ttattcttaa tggcctgttg agccatcagg aacgcagttc
     4021 acccccggtt tgcgatctgg tgtgtaaagg cgaaaacatt ctgtcttcct gtccgtcgat
     4081 atcggttgca ggtctggttg cctgagcgac tgggccatca ttgggctgga gacattgctt
     4141 tgcggtaggt cggctttatc agcagtaccg agcggaccag cataagcagg aagagcagag
     4201 actgataaca tcaaagcagc aaaataaggc ttcattttta ccacctttat caggttacgt
     4261 ttcatttgtt ccagaggaac attgtcgatt tttcgcgcat tgctggtggc tgggaatcac
     4321 ctgaatgggt gatttttgaa ttaccggctt tggtgcggtt tgtcttgccg gatgcgccgc
     4381 caggcgcggc ttatccggcc tacgggtagg tatatccggc tttggtggag gcgcgctcca
     4441 aatccaggtt gaacaaaaca tacacaaaaa atatagatct ccgtcacatt tttgcgttat
     4501 acaggaagct cgccactgtg aaggaggtac tgctatgacg tcactctctc gtccgcgcgt
     4561 ggagtttatc tccaccattt tgcagaccgt actcaatctt ggcctgctgt gcctcggcct
     4621 tattttggtt gtcttcctcg gcaaagaaac ggtgcatctg gctgatgtgc tgttcgcgcc
     4681 agaacaagcc agcaaatatg agctggtaga aggactggtg gtttactttc tctatttcga
     4741 atttatcgcg ctgattgtga agtactttca gtccggtttt cacttcccgc tacgttactt
     4801 tgtctatatt gggatcaccg ccattgtgcg cttgatcatc gtcgatcata aatcgccact
     4861 ggatgtgttg atctactcgg ctgcgatcct gttactggtg gtcaccctgt ggctgtgtaa
     4921 ttcgaaacgg ctgaagcggg agtaaaaaga cagcacgccg aactggcgcg gcgtgctgga
     4981 caggaagatt acagcgtagc agtttgttgt gttttcttcg tttccggttc ccagagggct
     5041 tccagctcct caagggtttt acctttggtt tccgggacaa atttccacat aaacagtgct
     5101 gccagaacgc ccatacaacc gtaaatccag taggagaaac cgttgtggaa atgggccacc
     5161 agccaggagt ttttgtccat catcgggaag gtccaggaga cgaagtagtt cgccagccac
     5221 tgggccgcca ccgcgattgc cagcgcttta cctcgaatag cattcgggaa gatttccgac
     5281 agcagtaccc agcataccgg accccaggac atggcaaagg cggcaacata gaacagcatc
     5341 gacagtagcg ccacaatacc cggtgcctga gtgtaaaacg cggtaccgag gctaaacata
     5401 ccgattgcca ttccgagtgc gccgataatt tgcagtggct tacgaccaaa tttatccacc
     5461 gtcataattg ccagaacggt gaaggtgagg ttgataactc cgacaataat ggtctgcaac
     5521 agcgcgatat ccgtgctggc ccccagcgtt ttgaacactt ccggcgcgta gtacagcacc
     5581 acattgatgc cgacaaattg ctggaagatg gagagcataa cgccgatgac aatcacgccc
     5641 acgccaaaca tcagcagacg accaccggtt ttgcggccat gatccaggga gtgtttaatt
     5701 tcctgtactg cctgagttgc aagcgtgttg cccataattt tgcgcaggat accttccgcc
     5761 tgttcttgct tgccgcgtga catcagccag cgaggacttt ctggcacggt atacagcagc
     5821 attaagaaca gcagtgcagg gatacattcc gaggcaaaca tataacgcca gccgtcagta
     5881 ttcagccagc tggcatcacc ggaacgcgca ataaaatagt ttacgcagta aactaaaagc
     5941 tgcccgaaaa taatcgcaaa ctggttaaat gaaaccagtt tcccgcgaat atgagcagga
     6001 gcaagttccg caatatacat tggtgaaagc attgaggcta aaccaacgcc aataccgcca
     6061 ataatacgat aaataacaaa ttccgggaca taacctgcca gataaacagg cactgtgttg
     6121 tccgggttta tagaggtaaa accaagttct ggccaggcag aacctacacc agaaataaaa
     6181 aacaggacag cagcaatctt aagtgaatca cgacgaccga agcggttact gcaataacca
     6241 ccgagggcac cgccgatgat gcaaccaatc agagcgctgg ccacgcaaaa ccctaacagg
     6301 gagttggcag cggattcact taagttttgt ggagcaacaa agacggtatt gagtgactca
     6361 acagtaccgg aaataacggc ggtgtcgtag ccaaataata aaccacctaa tgtagcgact
     6421 aaggtaatcg aaaatatata actggaatta tactgggtat tcattcagac ctgccttaga
     6481 ccattctgat cttttatcag tgcattgatg ttggaattgt caggcagtta aggcaattat
     6541 cattttttac aatgcgttta cgtaatgtct tttctgtgat cttaattgtg ataattatcc
     6601 aaaattgata aaaacaatac tattgccgtg actcagagca cgaaagagaa ttatcgtaag
     6661 tgggaaaaca aataacgtaa aaataataag ctctatgatg gaaatattaa ccggcgaacg
     6721 attcagattg cagacgaaag aaaaaaaggc gctccgtgga gcgccgaata acagtcacaa
     6781 gttgggataa cgtaagttga gggtgcagcg gcataacatt ggcagaacaa catctttaac
     6841 ctttcacacc acctgccgtc aggccgttga ccagccagcg ttgagccagc aagaagacga
     6901 tggtgatcgg taatgcagac atcacggcag cggcggcaaa gtcaccccac aggtagtttt
     6961 gcggattgag gtattgctgc atccccacgg ccagggtgta gctgtttacg tcacgcagta
     7021 acagcgacgc gaccggaact tcagtaatgg cagcgataaa cgacaggatg aataccaccg
     7081 ccagaatcgg tactgacagc ggcaacagga caaggcggaa ggcctgccac ggtgtcgcac
     7141 catccagcgc agccgcttct tccagcgaac tgtcgatggt ttcgaaatag cctttgatgg
     7201 tccagacgtg cagcgcaatc ccacccagat acgcgaaaat tacgccgccg tgagtattca
     7261 ggccaatgaa tggaatgtac tcacccagac gatcaaacaa cgcatacaac gcgaccagtg
     7321 aaagtactgc cgggaacatc tggaaaatca gcattccttt cagcagcgtc gctttgcctg
     7381 gaaagcgcat acgggcgaaa gcgtaggcgc aggtggtgga gagcgccaca atgccaatcg
     7441 cggaaatccc ggcgaccttt accgagttcc acagccacag cagcaccggg aatggcggtg
     7501 gcgtaatgcg accatcagcc tgttccacgc taaaacctaa tgccagtttc cagtgatccc
     7561 aggagatttg ctccgggatc aggctgccgg tcgcaaagtt cccctggcgc agtgagatag
     7621 cgacgaccat cagcagcggg aacataatcg ccgcgataaa aagtagcagt agcaggtgag
     7681 taataaacaa acgtgctttt tgcgatttcg gttggaccat tgccattttt gttatctccc
     7741 ttaatcaaac ttcattcgcg tggctttcag gttcactatc gccagcgcac ccaccagcag
     7801 gaagatcagc gtggcaattg ctgctgccag accgaagtcc tgacccccgc cgccttcaaa
     7861 agcgatgcgg taggtgtagt taacaagcag gtcggtataa ccggctggcg tggtcgtgcc
     7921 aagacgatcc gggccgccgt tggttaacag ttgaatcagc acgaagttgt taaagttaaa
     7981 ggcgaagctg gcgatcatca gcggcgtcag cggtttaatc agcagcggca gcgtaatctt
     8041 aaagaagttc tggaacggac ctgcgccatc cattgctgag gcttcataca aatcgtccgg
     8101 aatcgctttc agcaagccca tgcagaggat catcatgtac ggatagccca gccaggtatt
     8161 gacgataatc agcatcgttc gggcggtggt cggatcgctg aaccaggcgg gcttcacgcc
     8221 aaacagcgcg ctcaacatca tgttgatctc accgaagctc tggttaaaca accctttgaa
     8281 aatcaagatt gaaatgaacg atggcaccgc gtagggcaga atcagcagga cgcgatagac
     8341 cgctttgccg cgcaacgctt cccactgcac cagacacgcc agaaccatgc cgactgccac
     8401 cgttaaaaag acagtgatca gcgagaacac cacggtccag acgaaaatgg cgaggaacgg
     8461 tttctgaatg ccttcgtcgg tgaagacgcg agtaaagttt ttccagccgg tggtcacggt
     8521 gtaaccgggg cttagctttt catcacccca gttgccgtcg gcggtaatgg actggtaaaa
     8581 gccaatttgg ttattcggac gatatttcac gccgctctga ttattcgtca acgtgccgtc
     8641 accgtcgagt gtgtagagcg gctgcgtgcc agaaaactgg cgcagggagc tcatcatcac
     8701 tttgttgcca tccggcagaa tggcggtaat gtcactcagc gcctgacgat tctgggtaat
     8761 cacgcgtaga tttgcgcgtt cgccttcggg ctgggcggtc gtttctttca gttgcagttt
     8821 ttgctctctg ccaaatttaa aagcgtcgga gaggtaattt ttgccggttt cgccgtcgct
     8881 gagcgccagt tgccactcat cgcccgccgg gtaaagacca aagttatagg ttttgcctgc
     8941 ttgccaggag cgatctaaca acacttcctg tgcacgttca aaagtcagct ggttagtgct
     9001 gctgtagttg gtgaaggcaa tggcgatggt gcagaccaga gggaagagga cgaataatcc
     9061 cattccagcc attcccgggt aaacatagcg ccaggcgtag gctttacgat tggcgaaaat
     9121 atacagcccc gctgaactca atatcagcgt ggtaatggcg aacaggtatt ccccttgtgc
     9181 gtacattaaa acaacaaggt aacccaccag caggccgagc agacctaaca ctgaccattt
     9241 cagcgcgtcg ctttgccacc aatgtttctt tttaatgaca tccatggggt tcttcctcat
     9301 tccaggacgg ataaggcttt catgccttat ccgacaacaa ctgcctgatg cgacgctgac
     9361 gcgtcttatc aggcctacat acgtttcggt tttgtaggcc ggacaaggcg ttcacgccgc
     9421 atccggcatt tcacagcatt acttggtgat acgagtctgc gcgtctttca gggcttcatc
     9481 gacagtctga cgaccgctgg cggcgttgat caccgcagta cgcacggcat accagaaagc
     9541 ggacatctgc gggatgttcg gcatgatttc acctttctgg gcgttttcca tggtggctgc
     9601 aatacgtgga tctttcgcca actcttcctc gtaagacttc agcgctacgg cacccagcgg
     9661 tttgtcttta ttaaccgctt ccagaccttc gtcagtcagc agatagtttt cgaggaactc
     9721 tttcgccagc tctttgttcg gactggcggc gttaataccg gcgctcagca cgccaacgaa
     9781 cggtttagat ggttgaccct tgaaggtcgg cagtaccgtt acaccataat tcactttgct
     9841 ggtgtcgatg ttggaccatg cccacgggcc gttgatagtc atcgctgttt cgcctttatt
     9901 aaaggcagct tctgcgatgg agtaatcggt gtctgcattc atgtgtttgt ttttaatcag
     9961 gtcaaccagg aaggtcagac ccgctttcgc gccagcgtta tccacgccca cgtctttaat
    10021 gtcgtacttg ccgttttcat acttgaacgc ataacccccg tcagcagcaa tcagcggcca
    10081 ggtgaagtac ggttcttgca ggttgaacat cagcgcgctc ttacctttcg ctttcagttc
    10141 tttatccagc gccgggatct cttcccaggt ttttggcggg ttcggtagca gatctttgtt
    10201 ataaatcagc gataacgctt caacagcgat cgggtaagca atcagcttgc cgttgtaacg
    10261 tacggcatcc caggtaaacg gatacagctt gtcctggaac gctttgtccg gggtgatttc
    10321 agccaacagg ccagattgag cgtagccacc aaagcggtcg tgcgcccaga agataatgtc
    10381 agggccatcg ccagttgccg caacctgagg gaatttctct tccagtttat ccggatgctc
    10441 aacggtgact ttaattccgg tatctttctc gaatttctta ccgacttcag cgagaccgtt
    10501 atagccttta tcgccgttaa tccagattac cagtttacct tcttcgattt tggcgagagc
    10561 cgaggcggaa aacatcatcg tcgttaatgc ggataatgcg aggatgcgtg cacctgtttt
    10621 tattttcata atctatggtc cttgttggtg aagtgctcgt gaaaacacct aaacggactc
    10681 tagtttcttt atacggcaac ctctttccat cctccttgcc cctacgcccc accgttgctt
    10741 tgtgtgatct ctgttacaga attggcagta atgtggagat gcgcacataa aatcgccacg
    10801 atttttgcaa gcaacatcac gaaattcctt acatgacctc ggtttagttc acagaagccg
    10861 cgttctcatc ctcccgcctc ctcccccata aaaaagctag ggggtggagg atttaagcca
    10921 tctcctgatg acgcatagtc agcccatcat gaatgttgct gtcgatgaca ggttgttaca
    10981 aagggagaag ggcatggcga gcgtacagct gcaaaatgta acgaaagcct ggggcgaggt
    11041 cgtggtatcg aaagatatca atctcgatat ccatgaaggt gaattcgtgg tgtttgtcgg
    11101 accgtctggc tgcggtaaat cgactttact gcgcatgatt gccgggcttg agacgatcac
    11161 cagcggcgac ctgttcatcg gtgagaaacg gatgaatgac actccgccag cagaacgtgg
    11221 cgttggtatg gtgtttcagt cctacgcgct ctatccccac ctgtcagtag cagaaaacat
    11281 gtcatttggc ctgaaactgg caggcgcaaa aaaagaggtg attaaccaac gcgtcaacca
    11341 ggtggcggaa gtgctacaac tggcgcattt gctggatcgc aaaccgaaag cgctctccgg
    11401 tggtcagcgt cagcgtgtgg cgattggccg tacgctggtg gccgagccaa gcgtattttt
    11461 gctcgatgaa ccgctctcca acctcgatgc tgcactgcgt gtgcaaatgc gtatcgaaat
    11521 ctcccgtctg cataaacgcc tgggccgcac aatgatttac gtcacccacg atcaggtcga
    11581 agcgatgacg ctggccgaca aaatcgtggt gctggacgcc ggtcgcgtgg cgcaggttgg
    11641 gaaaccgctg gagctgtacc actatccggc agaccgtttt gtcgccggat ttatcggttc
    11701 gccaaagatg aacttcctgc cggtaaaagt gaccgccacc gcaatcgatc aagtgcaggt
    11761 ggagctgccg atgccaaatc gtcagcaagt ctggctgcca gttgaaagcc gtgatgtcca
    11821 ggttggagcc aatatgtcgc tgggtattcg cccggaacat ctactgccga gtgatatcgc
    11881 tgacgtcatc cttgagggtg aagttcaggt cgtcgagcaa ctcggcaacg aaacccaaat
    11941 ccatatccag atcccttcca ttcgtcaaaa cctggtgtac cgccagaacg acgtggtgtt
    12001 ggtagaagaa ggtgccacat tcgctatcgg cctgccgcca gagcgttgcc atctgttccg
    12061 tgaggatggc actgcatgtc gtcgactgca taaggagccg ggcgtttaag caccccacaa
    12121 aacacacaaa gcctgtcaca ggtgatgtga aaaaagaaaa gcaatgactc aggagataga
    12181 atgatgatta ctctgcgcaa acttcctctg gcggttgccg tcgcagcggg cgtaatgtct
    12241 gctcaggcaa tggctgttga tttccacggc tatgcacgtt ccggtattgg ctggacaggt
    12301 agcggcggtg aacaacagtg tttccagact accggtgctc aaagtaaata ccgtcttggc
    12361 aacgaatgtg aaacttatgc tgaattaaaa ttgggtcagg aagtgtggaa agagggcgat
    12421 aagagcttct atttcgacac taacgtggcc tattccgtcg cgcaacagaa tgactgggaa
    12481 gctaccgacc cggccttccg tgaagcaaac gtgcagggta aaaacctgat cgaatggctg
    12541 ccaggctcca ccatctgggc aggtaagcgc ttctaccaac gtcatgacgt tcatatgatc
    12601 gacttctact actgggatat ttctggtcct ggtgccggtc tggaaaacat cgatgttggc
    12661 ttcggtaaac tctctctggc agcaacccgc tcctctgaag caggtggttc ttcctctttc
    12721 gccagcaaca atatttatga ctataccaac gaaaccgcga acgacgtttt cgatgtgcgt
    12781 ttagcgcaga tggaagtcaa cccgggcggc acattagaac tgggtgtcga ctacggtcgt
    12841 gccaacctgc gtgataacta tcgtctggtt gatggcgcat cgaaagacgg ctggttattc
    12901 actgctgaac atactcagag tgtcctgaag ggctttaaca agtttgttgt tcagtacgct
    12961 actgactcga tgacctcgca gggtaaaggt ctgtcgcagg gttctggcgt tgcatttgat
    13021 aacgaaaaat ttgcctacaa tatcaacaac aacggtcaca tgctgcgtat cctcgaccac
    13081 ggtgcgatct ccatgggcga caactgggac atgatgtacg tgggtatgta ccaggatatc
    13141 aactgggata acgacaacgg caccaagtgg tggaccgtcg gtattcgccc gatgtacaag
    13201 tggacgccaa tcatgagcac cgtgatggaa atcggctacg acaacgtcga atcccagcgc
    13261 accggcgaca agaacaatca gtacaaaatt accctcgcac aacaatggca ggctggcgac
    13321 agcatctggt cacgcccggc tattcgtgtc ttcgcaacct acgccaagtg ggatgagaaa
    13381 tggggttacg actacaacgg cgatagcaag gttaacccga actacggcaa agccgttcct
    13441 gctgatttca acggcggcag cttcggtcgt ggcgacagcg acgagtggac cttcggtgcc
    13501 cagatggaaa tctggtggta atagcaaaac ctgggccgga taaggcgttt acgccgcatc
    13561 cggcataaaa caggttgtca ttatctgaaa ggggcgaaag cccctctgat tatcgggttt
    13621 agcgcgctat tgcctggcta ccgctgagct ccagattttg aggtgaaaac aatgaaaatg
    13681 aataaaagtc tcatcgccct ctgtttatca gcagggttac tggcaagcgc gcctggaatc
    13741 agccttgccg atgttaacta cgtaccgcaa aacaccagcg acgcgccagc cattccatct
    13801 gccgcgctgc aacaactcac ctggacaccg gtcgatcaat ctaaaaccca gaccacccaa
    13861 ctggcgaccg gcggccaaca actgaacgtt cccggcatca gtggtccggt tgctgcgtac
    13921 agcgtcccgg caaacattgg cgaactgacc ctgacgctga ccagcgaagt gaacaaacaa
    13981 accagcgttt ttgcgccgaa cgtgctgatt cttgatcaga acatgacccc atcagccttc
    14041 ttccccagca gttatttcac ctaccaggaa ccaggcgtga tgagtgcaga tcggctggaa
    14101 ggcgttatgc gcctgacacc ggcgttgggg cagcaaaaac tttatgttct ggtctttacc
    14161 acggaaaaag atctccagca gacgacccaa ctgctcgacc cggctaaagc ctatgccaaa
    14221 ggcgttggta actcgatccc ggatattccc gatccggttg ctcgtcatac caccgatggc
    14281 ttactgaaac tgaaagtgaa aacgaactcc agctccagcg tgttggtcgg gcctttattt
    14341 ggttcttccg ctccagctcc ggttacggta ggcaacacgg cggcaccagc tgtggctgca
    14401 cccgctccgg tgccggtgaa gaaaagcgag ccgatgctca acgacacgga aagttatttt
    14461 aataccgcga tcaaaaacgc tgtcgcgaaa ggtgatgttg ataaggcgtt aaaactgctt
    14521 gatgaagctg aacgcctggg atcgacatct gcccgttcca cctttatcag cagtgtaaaa
    14581 ggcaaggggt aattacgccc cacagtgctg attttgcaac aactggtgcg tctcctggcg
    14641 cacctttttt tatgcttcat tccagggata tgagcgattt tttatagtaa ctcacttctt
    14701 cttcactaag aatatccatt atctcaatgc attatcagag attcttttcc tttcgccggt
    14761 agtgtctgga cgctcaggct acttt
//
LOCUS       DBFIFI010000102        14785 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.102, whole genome shotgun sequence.
ACCESSION   DBFIFI010000102 DBFIFI010000000
VERSION     DBFIFI010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14785
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.102"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(171..236)
                     /locus_tag="N7B39_002955"
                     /pseudo
     CDS             complement(171..236)
                     /locus_tag="N7B39_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444509.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            complement(247..352)
                     /locus_tag="N7B39_002956"
                     /pseudo
     CDS             complement(247..352)
                     /locus_tag="N7B39_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
     gene            532..705
                     /locus_tag="N7B39_002957"
                     /pseudo
     CDS             532..705
                     /locus_tag="N7B39_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            816..1739
                     /gene="yicL"
                     /locus_tag="N7B39_002958"
     CDS             816..1739
                     /gene="yicL"
                     /locus_tag="N7B39_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate/amino acid/amine transporter"
                     /protein_id="HGU2422907.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLIGTFLLVTHGNPTSLSISPAALF
                     WGIASAFTAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYAGQGTNFIVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(1743..2561)
                     /gene="nlpA"
                     /locus_tag="N7B39_002959"
     CDS             complement(1743..2561)
                     /gene="nlpA"
                     /locus_tag="N7B39_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HGU2422908.1"
                     /translation="MKLTTHHLRAGAALLLAGVLLAGCDQSSSDEKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNNGELDANVFQHRPFLEQDNQTHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIEQTGLSPVNDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            2783..3076
                     /gene="yicS"
                     /locus_tag="N7B39_002960"
     CDS             2783..3076
                     /gene="yicS"
                     /locus_tag="N7B39_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HGU2422909.1"
                     /translation="MKPTMLLMITVFLIFPALSQAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(3117..4307)
                     /gene="nepI"
                     /locus_tag="N7B39_002961"
     CDS             complement(3117..4307)
                     /gene="nepI"
                     /locus_tag="N7B39_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HGU2422910.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGCFLGELIGWRNVFNAAAAMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMTLAGFGVDGLTLVLLSFGIASFVGTSLSSFIL
                     KRSVKLALAGAPFVLALSALVLTLWGSDKIVATGVAIIWGLTFALIPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            4541..4894
                     /locus_tag="N7B39_002962"
     CDS             4541..4894
                     /locus_tag="N7B39_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGU2422911.1"
                     /translation="MFKTKWFAREARSHAITDEELCRAILETEQGKADALGGGVFKKR
                     LHQNRERAIILAKGVSNWFYTFLYAKQDMSNINSQELAGFREIAKHYAFLTKAQLTAM
                     INTKELTEICYDCKN"
     gene            4878..5201
                     /locus_tag="N7B39_002963"
     CDS             4878..5201
                     /locus_tag="N7B39_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU2422912.1"
                     /translation="MTAKTKFKSPAFEAIHSAAAGLSSVNAISAETMRTFDKACLTSV
                     QDLQPVEIKALREKLKVSQPVFAHYLNTSVSTVQKWESGAKRPNGISLKLLSIVQKHG
                     LEVLL"
     gene            complement(5317..5769)
                     /gene="yicN"
                     /locus_tag="N7B39_002964"
     CDS             complement(5317..5769)
                     /gene="yicN"
                     /locus_tag="N7B39_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HGU2422913.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(5822..7155)
                     /gene="adeQ"
                     /locus_tag="N7B39_002965"
                     /gene_synonym="yicO"
                     /pseudo
     CDS             complement(5822..7155)
                     /gene="adeQ"
                     /locus_tag="N7B39_002965"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
     gene            7330..9096
                     /gene="adeD"
                     /locus_tag="N7B39_002966"
     CDS             7330..9096
                     /gene="adeD"
                     /locus_tag="N7B39_002966"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HGU2422914.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYTDAPALQRIDAHGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESAKLAQSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFERNDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(9142..10533)
                     /gene="uhpT"
                     /locus_tag="N7B39_002967"
     CDS             complement(9142..10533)
                     /gene="uhpT"
                     /locus_tag="N7B39_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HGU2422915.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(10671..11990)
                     /gene="uhpC"
                     /locus_tag="N7B39_002968"
     CDS             complement(10671..11990)
                     /gene="uhpC"
                     /locus_tag="N7B39_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HGU2422916.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVIAIAAGISALLLLPFLNAQTPREA"
     gene            complement(12000..13502)
                     /gene="uhpB"
                     /locus_tag="N7B39_002969"
     CDS             complement(12000..13502)
                     /gene="uhpB"
                     /locus_tag="N7B39_002969"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HGU2422917.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFSLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIEESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWLQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLTISCLHGTRVSVSLPQRYV"
     gene            complement(13502..14092)
                     /gene="uhpA"
                     /locus_tag="N7B39_002970"
     CDS             complement(13502..14092)
                     /gene="uhpA"
                     /locus_tag="N7B39_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HGU2422918.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(14254..>14785)
                     /locus_tag="N7B39_002971"
     CDS             complement(14254..>14785)
                     /locus_tag="N7B39_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2422919.1"
                     /translation="VVFTPGECDLVRDSLVQDGIKPEKIAKIISCLSSRTGIRNLFYA
                     CSQAFHVANDNAALDVRRAATSVGDTIDVCPSFVALNGRNYHQHVDFSEYGFTQTLCV
                     LGAVVNNANDTFDVANYIYTTKGENIIHNDAEDDSPYVHSDESYRHKDDYWARDNDIR
                     NLRRNFSGNITIATYK"
BASE COUNT         4001 a         3924 c         3514 g         3346 t
ORIGIN      
        1 tgttcacacc acatctggta tagaaaagca caggctagca aaaacctgaa taaccccgta
       61 cttacggagt tattcaggtt aaaaatatcc ataacatcac ttctgatttt tattgcttac
      121 gacggatata atttctctgg tcagctacta atatcatcat gctcacgcgt ttaatcaaac
      181 tcgtgtttct caaacggaca tcgcgggaca atcttctgct tctcccggcg cattttttac
      241 caccttcgct acctgcattt tgagcggaag atccgctttt ttctggcccc cgcacatatg
      301 caagtccagt tttaaattta tcctttattt cattctgaaa caaactgctc ataattttct
      361 ccagtggaac ctgcatctca gggagacatc actacagaat acttcccctt atgcactgat
      421 ttaattttgg tcatttagaa taacggcctg taaagtctca atgcaacaga tgggtaacgc
      481 tattatgagg atcctcgtca gtaaattact ggcaaggcac tccgataaat aagaataggt
      541 tccgcaacaa cgttatatgc aaatacctca cgcttcggct agattatcgc cggctctgtt
      601 gacgaaatta tggttgaaat ctggagctac cacacgttgt tctggctggc gatagggatg
      661 ttgggtattg caatgatttg cttgctgttt attaaagata tttagctcac cagtctgggc
      721 taagaaaaat cggcgtggcg caaactgagc catcctcgcc atttttgcta tcatgcctgc
      781 atacataaac gacaaaacag tatgcagagg gaaaaatggg ttccaccaga aaggggatgc
      841 tgaacgttct gattgccgcc gtgttgtggg gaagttcagg ggtctgcgcg caatacatca
      901 tggagcaaag ccagatgtcg tcgcagtttt tgactatgac gcgtttgata ttcgccggtt
      961 tgattctgct gacgctatca tttgttcatg gtgataaaat cttttctatt attaacaatc
     1021 acaaagatgc cattagccta ctgatttttt ccgtggttgg cgcgctaacc gtacagctca
     1081 cttttttgct aaccatcgaa aaatcgaacg cagccacggc aacggtgctg caattcctct
     1141 caccgactat tatcgtcgcc tggttctcac tggtgcgtaa atcccgcccg ggcattctgg
     1201 ttttctgcgc tattttgaca tcgctgatcg ggactttttt attggtgaca cacggtaatc
     1261 cgacgtcatt atcgatctct cctgccgcgt tattctgggg cattgcctcg gcatttactg
     1321 ctgcattcta taccacctat ccctcaacgc tgattgcccg ctatggcacg ttaccggtcg
     1381 tcggctggag tatgctcatt ggcggtctga tcctgctgcc cttttatgcc ggacaaggga
     1441 caaactttat cgttaacgga agtttgattc tggcgttttt ttatttggtg gtcattggta
     1501 cgtccctgac atttagtctg tacctgaaag gagcacaatt aattggcggt ccaaaagcca
     1561 gcattttaag ctgtgctgaa ccattaagta gcgcgctgct ctctttgctg ttgctgggga
     1621 tcaccttcac attgccggac tggctgggaa cgctgctgat tctgtcatcg gtgattttga
     1681 tttcaatgga ttcccgccgc cgcgccagaa aaataaatcg tccggcgcgg catgagtgag
     1741 aattaccagc caggcaccgc accaccgtta aagatggttt ctgccgcttt agcgacttcg
     1801 ggtgattgat aagattgcag aaattccttc acgttttctg cattcttatt atcttcccgt
     1861 gccaccagaa tattcacata cggcgaattt ttatcttcaa taaatacgct gtcgttaacc
     1921 ggagaaagtc ctgtttgctc gatgtaagtg gtgctgataa tcgctacatc cactttcggg
     1981 tcgtccagta cacgtggcaa ctgtgcccct tccagctcca ttatctgcaa atggcgtgga
     2041 ttatcggtta tatccaacgc ggtaggtaat aacccttttc cctctttcag agtaattagc
     2101 ttctcttttt gcagcagtaa aagcgcgcga ccaaggttag tcgggtcgtt ggggatcgcc
     2161 actgtcgcac cttcttttat ttgcgccacg gttttgattt ttttggaata gcccgccata
     2221 gggaagacaa acgtatttcc caccgctacc agtttataac catgcgtctg attatcttgt
     2281 tcaagaaagg gtcgatgctg gaagacgttc gcatcaagct cgccattatt tgtcgcatcg
     2341 ttcggtagca acgaaccgct aaatcctacc agttcaacat cgaggccata tttctctttt
     2401 gctacctttt tagcgacttc tgcgacatct tgttctgcgc cattaataac gccaacttta
     2461 atatgctttt catcgctgct gctctggtcg caacctgcca gcagaactcc tgccagtaat
     2521 aatgcggccc ccgcccgtag atgatgtgtt gtcagtttca tttttttatc cttttattaa
     2581 tgattaccgg actatagcgg taaggtcgct gcagtttaaa aaacaaaacg ctatcgataa
     2641 gaataaaaag gaataaaagt ggaatataaa gtctggcact tcttactgac cacgcaaaca
     2701 cctttcgtac agcatgatga gagcggcaaa agcaaactac acttatgctt cattcgatgc
     2761 acatttgcag aaggttgccg ctatgaagcc aacgatgcta ctcatgatta ccgtattcct
     2821 gatttttcca gccctttctc aggccgagtc gccattcagt tctctacagt cggcaaaaga
     2881 gaaaaccacg gtattgcaag atttacgtaa aatttgcacg ccacaggcgt cattatcaga
     2941 tgaagcgtgg gaaaagttaa tgttgtcaga tgaaaacaat aaacaacata tacgcgaggc
     3001 catcgtggcg atggaacgta ataatcagag caattattgg gaagcgttgg gtaaggtaga
     3061 atgcccggat atgtaagaac ggcaacaacc actgaccaac gcttctttac ctgatatcag
     3121 gatttcttca ttttcacctt tgcagtcacc aacaatgcag tcagcaacat caatgtgccg
     3181 gacaacatca gcggcgaagt cagaccaatg ttatccaacg cataaccgcc gattgccgca
     3241 ccgcaggtat tggcaagctg aataacggca acctgaatag atccagcctt ttctgcctga
     3301 tcggccaatg agcgagtgat ccacgttgac caaccaacgg gaattaaagc aaaggtcagc
     3361 ccccagataa tcgccacacc agtagcaaca attttatcgc ttccccacag cgtcagtacc
     3421 agcgcgctca gggcgagcac aaacggtgcg cccgctaagg ccagtttcac cgaacgtttg
     3481 agaataaacg acgaaagcga tgtcccgaca aagctggcga taccaaaact cagcagcacc
     3541 agggttaagc catcaacacc gaatcccgcc agagtcatat acactgggcg aatataagtg
     3601 aaaaaagcaa actgtccggc aaaggacata aagatggcga tcatcccggc catcactccc
     3661 ggacgttgta ataagcggaa agtattttgt ttctgatgcg aaggttcacc tggcagcgaa
     3721 ggcagtgatt tgataatcca gaaaatacac agcacaccca tcgccgccgc cgcattaaag
     3781 acattgcgcc aaccgataag ctcgcctaaa aaacagccca acggcgcggc aatcaccagc
     3841 gcaatagaaa ccgcgccgaa gatcaccgac agcgccttcg gcaccgtacg cggcggcacc
     3901 agacgcatgg tcagcgacgc cgacatcgcc cagaacccgc ccagcgccag ccccagacag
     3961 gcacgaccga ttaaaagcaa actgaatgag ttagcaaagg aaaccagcaa gcaggagagc
     4021 gtcagcaaaa cggcaaacaa aataacaacg tagcggcggt cagtggcctg aattgtctgg
     4081 gtaataaaca aactggcaaa cattgccaca aaggcggtca cggtcaccga ttgcccggca
     4141 accccttccg aaatgcctaa atcctgggcc attggcgtca acaaactgac aggcaaaaac
     4201 tcaacgataa tcagacaggc gacgcaaaac gccaccgaga aaacggctga ccagttcggt
     4261 cgggtgatgg catccgcgcc gcggttttcg gcaataaatt cactcatggt gttacccgtg
     4321 gcatgtttca ggggaaaagc cgaccacttt aacattgaaa tgtgtgacgc atttaacgtt
     4381 tttgcaactt ttacggtgtt gtgggaaaag ctcgaggctg ctcgaagaaa taaaaatcat
     4441 aggacaaggt gatatgtatt ttcgcgattg tgttgggaac aagggataaa gtattgcact
     4501 tggtgatata ctttaataac aggacctcaa cgatatacgc gtgtttaaaa ccaaatggtt
     4561 tgcaagggaa gccaggtctc atgcaataac agatgaagag ttgtgccggg ccattttaga
     4621 aacagaacaa ggtaaagctg atgcacttgg cggtggcgtt tttaaaaagc gccttcacca
     4681 aaatcgtgag cgtgcaatta ttctggcgaa aggggtaagc aattggtttt acacgttttt
     4741 atatgcaaaa caggatatgt cgaacatcaa tagtcaggaa ctggctgggt ttcgtgaaat
     4801 tgcaaagcat tatgccttcc tgacgaaagc ccaactaaca gcaatgatta acactaaaga
     4861 actgacagag atctgctatg actgcaaaaa ctaaatttaa aagtcctgca tttgaggcta
     4921 ttcacagcgc agcggctgga ttgtccagcg tcaatgctat ttctgctgaa acgatgcgta
     4981 cttttgacaa agcctgcctg accagcgtgc aagacttaca acctgttgag ataaaagcac
     5041 tgcgggaaaa attaaaagtg agtcagcccg tcttcgctca ctatctcaat accagcgttt
     5101 caactgtaca aaaatgggaa agtggcgcta aacgccccaa tggcatttca ttaaaactgc
     5161 tcagcattgt gcaaaaacac gggttggagg tgctgctgta aatagaacgt agataatgcg
     5221 ttcacctact gtcagttccg ataccggatg cggcgtgaac gccttatcca ccctacaggt
     5281 caggcgcggt ctgtaggctg ataagacgaa gcggttttaa tgcagccagt gaacgccctc
     5341 ggcaggctga aagaacgcgt tatagcgcat ctgaaaagca ttaatatcct gcatttcagg
     5401 ttcggtttcg cgcagaaaac ccagcgccag cctgacctga gcatcagtaa tgggagcggc
     5461 aaggcgggct ttttgtaaaa tccgatgcca ttgcagcagg ttctgttcgc taccatcgac
     5521 aatgacaatt tgccagatga gcaacacctg cgcggcgttt tgcaggtgcg actcatccgg
     5581 acgatgcaaa taacgtaaaa attcgtcacc ggctgacttt cccatttgat cgaccatcga
     5641 ctccacgggt acgacatcga tattcagccg atcgctgaga cggcgaatcg cttttctggc
     5701 cccggatgtc agcacccgaa aaccaaccac aaacactacc gccagcgtgg cgagcattat
     5761 ccagatcatg agttctcctg cgtaatcaga actcatgata tctggaaacc ctcgccagtt
     5821 actaatccac cagaataatc ttcagtgcaa acagagctgc gaccaccacc acacacaggt
     5881 tcagatcgcg ccagcgcccg gtgcacactt tcatgatgca gtacgacata aagccaagtg
     5941 caatcccttc ggtgatcgag aaagtaaagg gcatcatcac cgtggtaaca aacgcaggca
     6001 ccgattcggt aaaatcatcc cagttaacgc gcgccagact tgaggtcatc aacacgccaa
     6061 caaagattaa cgctccggcg gttgcgtaag gaggaaccat cgccaccagc ggtgagaaga
     6121 acataaccaa caggaacata acgccgacca caaccgcagt cagccccgtg cgaccaccga
     6181 ctgccacacc agaagtactt tcaatatagg cggtaacaga cgaggtgccg ataaacgcgc
     6241 ccgccaccga actacgctat caacatatag cgccttattc atattgggga atttaccgtt
     6301 actatctatt aagccagctt tatcagttac accaattaat gttcctgatg aatcaaatag
     6361 gttgatcagc ataaaggaga aaatgatacc ggcgagttca agtgttaacg cgccgctcaa
     6421 atctacttca ccaatgacgc cgctaatatc aggcggaatg gaatagacgc cgctaaaatg
     6481 aacatcaccg aaaaataatc cacagcaaga cgtcaccaca atagaaacaa gcaccgcggc
     6541 atgaaaatga cgtgatgaca acacggttat aataaaaaac cctaaaatac ctaacaacac
     6601 gccgtgagaa cttaaatcgc caatcatcac cagagtgtct ttattggcga caataacgcc
     6661 agtatttttt aatcccatta aggcgataaa taatccgatt ccgctggtga taccaatacg
     6721 taaacttaat ggaatgttgg agatcatcca gtaccggata cgaaatagcg tgagcaaaaa
     6781 tagcccaatt gcgccccaga atatcgcgcc catcccggtc tgccaggaga tacccatcgc
     6841 ccccacgacc acgaacgcaa agaaggcgtt cagccccatt gccggagcca gcgccacggg
     6901 taagttagca aatatcccca tcgcaatact gccgataccg gcaatcaaac aggtggtaac
     6961 aaacaccact ttcgggtcca tttgtgccgc gccgaggatt tgcgggttca caaaaacgat
     7021 gtacaccatg gttaaaaaag tggtcatccc cgcaatcaat tctgtgcgga cggtggtccc
     7081 atgctgaggt agtttaaata atcgcgaaag cgtccctgat tcattactca cgtaatcggt
     7141 attgtcatta ttcatttttt tgtccattgg aggagattta atcccttgct tttgtaaaac
     7201 aaatgacaac aacggttcag tgataattat cacatttcaa ttgcacatta atggatattc
     7261 tttaataatc ttgcgacatt tctttatgat aaataacaat caaattaata aaatcaaaat
     7321 gagaaaaata tgaataattc cattaaccat aaatttcatc acattagccg ggcggaatac
     7381 caggaattgt tagccgtttc ccgtggcgac gctgttgccg attatattat tgataatgtc
     7441 tctattctcg acctgattaa tggcggagaa atttccggcc caattgtgat taaagggcgt
     7501 tacattgccg gtgttggcgc agaatacact gatgctccgg ctttgcagcg gattgatgcc
     7561 cacggcgcaa cggcggtgcc agggtttatt gatgctcacc tgcatattga atccagcatg
     7621 atgacgccgg tcacctttga aaccgccacc ctgcctcgcg gcctgacaac tgttatttgc
     7681 gatcctcatg aaatcgtcaa cgtgatgggc gaagccggtt tcgcctggtt tgcccgctgt
     7741 gccgaacagg caaggcaaaa ccagtactta caggtcagct cttgcgtacc cgctctggaa
     7801 ggctgcgatg ttaacggtgc cagttttacc cttgaacaga tgctcgcctg gcgggaccat
     7861 ccgcaggtta ccggccttgc agaaatgatg gactaccctg gcgtaattag cgggcagaat
     7921 gcgctgctcg ataaactgga tgcatttcgc cacctgacgc tggacggtca ctgcccgggt
     7981 ttgggtggta aagaacttaa cgcctatatt gctgcgggta ttgaaaactg ccacgaaagt
     8041 tatcagctgg aagaaggacg ccggaaatta caactcggca tgtcgttgat gatccgcgaa
     8101 gggtccgctg cccgcaatct caacgcactg gcaccgttga tcaacgaatt taacagcccg
     8161 caatgcatgc tctgtactga tgaccgtaac ccgtgggaga tcgcccatga aggacacatc
     8221 gatgccttaa ttcgccgcct gatcgaacaa cacaatgtgc cgctgcatgt ggcatatcgc
     8281 gtcgccagct ggtcgacggc gcgccacttt ggtctgaatc acctcggctt actggcacct
     8341 ggtaagcagg ctgatatcgt cctgttgagc gatgcgcgta aggtcacggt gcagcaggta
     8401 ctggtgaaag gcgaaccgat tgacgcgcaa accttacagg cggaagagtc ggcgaaactg
     8461 gcacaatccg ctccgccata tggcaacacc attgcccgcc agccagtttc cgccagcgac
     8521 tttgccctgc aatttacgcc cggaaaacgc tatcgggtca ttgacgtcat ccataacgaa
     8581 ttgattacgc actcccactc cagcgtctac agcgaaaatg gttttgagcg caatgatgtg
     8641 tgctttattg ccgtacttga gcgttacggg caacgtctgg ctccggcttg tggtttgctc
     8701 ggcggctttg gtctgaatga aggcgcgctg gctgcgacgg tcagccatga cagccataat
     8761 attgtggtga tcggtcgcag tgccgaagag atggcgctag cggtcaatca ggtgattcag
     8821 gatggcggcg ggttgtgcgt ggtacgtaac ggccaggtcc aaagccatct gccgttaccc
     8881 attgccgggc tgatgagcac cgacacggcg cagtcgctgg cggagcaaat tgacgccttg
     8941 aaagccgccg cccgtgaatg cggtccgtta cccgatgagc cgtttattca gatggcgttt
     9001 ctttccctgc cagtgatccc cgcgctgaaa ctaaccagcc aggggctgtt tgatggcgag
     9061 aagtttgcct tcaccacgct ggaagtcact gaataataaa aaaagcccgg cgtcatgccg
     9121 ggcaaaagtc accagttacg tttatgccac tgtcaactgc tgaatttttt tctcgcggcg
     9181 gattttgcgt tcttccatca ccgccactat cgccatcagg cagatacaac caatcgcggc
     9241 gatatccagc gcagcgaagg tgcctgccca gccggtaagg ccgaatactg gcgtcccatc
     9301 ggcaatcatt cccagaccta acttggcaaa gctgtcacca atcaggtaag cgaaggtgcc
     9361 tttaatacca tcggcagcgc caatcgcttt tttaggtaca aagccaacag cagccacacc
     9421 aatcaacaat tgcgggccaa agaccaggaa acccaacgca aagagagaag ccagatagat
     9481 atattcgttg ctggcatgtt ggtacacacc gagcgtggcg ataatcagcg ccagcgcgat
     9541 gcaggccacc aggccacggc gaccgttcgc taggtcagag agccagcccc acagcagcgt
     9601 accgaccagc gcaccagctt caaacagcgt aaagccctga atcgccaccg ctttagagag
     9661 tttcagttcc tggaacgcgt acacagttga ccactggtcg ataccaatac gcaccacata
     9721 gaggaaaatg ttggcgaagc acagcagcca gatcactttg tttttcagca catactcaac
     9781 aaagatctgc cacttggtca tatcggtaga ttctgtctct ttgtcctctt cgctgatctc
     9841 ctcgccgaac agctcttcag ctttgccgag gccataagat tccggggagt cgctgccgta
     9901 acgcaggccg ataaaaccga caatcagcgc gataatcgac gggaagataa acatgccgat
     9961 gacatggcca tcgaacaggt aatttgcgcc gaacagtgcc acaccagctg cgcctgcacc
    10021 gccaaggttg tgagaaatgt tccagaaacc gaggaacgtc ccgcgtttac gacgcggcgt
    10081 ccatttggtg atggtggagt aactgcacga acccccggta ctctggaaaa agccacttaa
    10141 ggcgtagaag gcaatcatca ggaacaggct aaccgagccg ctgcccatac tggcgctaaa
    10201 gcccagcata caaatggcag acaggatcag catgaacggc aggaattgtt tggtgttttt
    10261 gccgtcggcg tagtaggaaa ccagcgtttt acccacgcca taagtgatgg agaaacccag
    10321 gccgatcatc cccagctgcg tcatgctcaa cccgtaggtc gaaatcatat cgttctgcgc
    10381 gatgttaaag ttcttgcgaa tcaggtacat cgtcaggtag ccgataaaga ccaccaggta
    10441 ggattgcatg aacggtttga accacatttt gcgccgcact tcgagcggaa ggtccagggt
    10501 cggcttgcgg acctggttta agaaagccag catgggttac tcctgaaatg aatacctgcg
    10561 tgaggcatgc attgtaaaaa tcagcagcaa atcaggcctg agatagcgtc caggcaaaac
    10621 ctggaaaatt tcttagtttt gccggatatg aagtgaaaag gtgagatgca tcacgcttcg
    10681 cgcggtgtct gggcgttcaa aaagggcagt aacagcagtg cggaaatccc ggcggcaatg
    10741 gcgataacca caaaaaatcc gctccagtgc caggtatcga gcactttcgc cagcggccaa
    10801 ccagcaagcg acgcccccag ataagcaaac aagccgacaa accccgtcgc cgcccctgcc
    10861 gcctctttgt gggaacattc tgccgccgcc atgccgatta acatctgcgg gccaaagaca
    10921 aaaaaaccaa tggtgaagaa gcaggttgcc tgcatcacgt agctggcaaa tggcatcagc
    10981 cacagcgagc cgactgaaag caaaattccg gcagcgaaaa tcaaattcat cggcccgcgg
    11041 ttgccgttaa acagtttgtc cgagccccaa cccgctacca gcgcaccgat aaatccgccc
    11101 agttcaaaca tcgtcactgc cgtattcgcc gtgaccagat cgacgcccag cgtctcggac
    11161 atatacaaat tgccccagtc gttgatcgcc gcccgaacca catagaccag cacatagcaa
    11221 aacgaaagca gccagatata cggattcaac aacacatact tggtgaggat ctctttacgc
    11281 gtcagccctg ccccttcttg ttgttgagca atctccagcg cgtcgtgtcg ccattcaccg
    11341 accgctggta aacctaacgc ctgcgggcga tcgcgcagcc gccagcagag aaaaatcccc
    11401 acgacgatcg ccatacaacc agcaatcatc atcccggcac gccagccgta atgcagcgca
    11461 gctgctgcca tcacaatggg aatgagcgca ccgccaacgt tatgcgccgt gttccataat
    11521 gcccaccaac caccgcgttc ggtacgagaa taccaggccg ttaacagacg cgcacacacc
    11581 ggtgaacccc agccctggaa aaaggcgttc agcacccaga gcacggcaaa cgcccatagc
    11641 gacgtcgaga agccaaacag aatgttgatg atgcccgtgg cgataagccc tatccccata
    11701 aaataacggg catttgagcg gtcgctgaca atgccggaga caaacttcga cacgccgtag
    11761 gtaatgtaaa acagggtcgc taacaggccg atatcgctac ggctgagcac gccattagca
    11821 aggatttctg gtacggcggc gttaaaactt ttacgcgtga agtaaaacag cgcgtaaccc
    11881 agccagatgg tcagcagaat atgccgacgc cagtagcgat aacgggcatc aatttcatgt
    11941 ttatcagtca ttaatggcgc atcggcaggc gctttcagaa acggcaacat catcaaacct
    12001 tagacatagc gttgaggtag agaaacgctg acacgcgtgc cgtgcagaca ggaaatggtc
    12061 aaggtgccgc ccagcgccgt tacgcgctcg cgcattccgg tgaggccaaa accttgttgc
    12121 ccggaacccg gcggtaaacc gctgccatcg tcttcaatca ccagcatcaa ccgttcatcc
    12181 tgcagccagc cttgcagggt gaccgcgctg gcatcagcat gtttcacaat gttattcagc
    12241 ccttcctggc agacacgaaa cagcgtcacg cgctggtttt cgcttaacgc tgactcttcg
    12301 attcgccatt cgagatggct gacaatgccg cgcccttcca gctccatttc ccgcatcagt
    12361 gagcggatgg cctgctctag ggtgagatca tccaactggc gcggacgtaa ccgccctaac
    12421 aaacggcgca ccgcgtcgta cacgcccagc gacagttgtt cgatgagctg cccgctctgc
    12481 ttcacactgg cgttatctgc cgccagtcgc tggacaatgc ccgcttgagt acgaatggca
    12541 gtgatggtct ggccgatatc atcatgcagc tcacgcgcca catcacggcg cacgctctct
    12601 tcagtttcta acaaacgttc agcgagatgc tgattgcgcg ccagttcctt ttgcagcgac
    12661 tggttaagtt cacgcaaccg ctggatgcca gcgcccagta acaaccctgt cagactttgc
    12721 accagcagcg agagcaataa atccacagga tgatcgcgcc aggtttgact ggcgatcagc
    12781 gcgatggcgt tcatcaatgt cgctatcagc gccccttgcc agccgtagtg ccaggccagc
    12841 gcgataatcg gcagcgccag gcagaatggc gtaaagcgcg acagttcgtc cggcaagccc
    12901 aactggagcc agagactgat aacaaacagc agcaagtacc agaccagatg ccgcccgcgc
    12961 cagttgatcg gttgagaaac taacgacggc ccgagcggca gccaggtgtt attggcgaga
    13021 taatgccaga acaccagaca tatcggggcc agcgtcaggc cgccagttaa agttaacagc
    13081 agcgcattcc acgactcttt gccgtgccaa agccagggca gcgactgcaa caacgccgcc
    13141 gccgttaacg ccgcaccctg taacagcaag gtgcgccagt cacgttgatg gcgatagcgc
    13201 gagatcagcg ccacgggcag taacgtcagt aaactaccga tcatcaataa ggagaaatgg
    13261 gttaaaccga ccgcctgcgt tagccagtaa atcagcagcc actccgctcc cagcagcacc
    13321 ggccagtatc cgcgcgggca ttgcagcatt agccctagcc gcagaccaaa cggaaataac
    13381 agcaccgcca tatcagggcg ctcaaccagg tgcaggctga tactccacag gcaaaaccat
    13441 gcggcagaga agataaaaaa gcaggcaata acggtaatta agcgggagaa caacgtcttc
    13501 atcaccagcc atcaaacata cggcgcgcca gttcaacgtc gttactgacg cccagttttt
    13561 ccatcagatt ggcgcgatgg acgtgtaccg ttttcggtga caagcccagt tcggcggcaa
    13621 tctctttcac cgccattcct tgcgccagtt tttccgccac ctgccgttca cgtttggtta
    13681 gtgggtcctg gcgaccggat gccagtttaa tggcaatatc cggcgtcaga taacagccgc
    13741 ctgtggcaac cgtatgcacc gcagcaatca gttcgtcagg gctacaacgc ttggagagaa
    13801 agccacgcgc ccccgcgtta agcgcctgct caaccagcgc cggactgtca tgaacggaga
    13861 gcataattgt cgccatacct ttcggcagct ggcttagcag ctccagaccg gagatatcgg
    13921 gcatggagat atcgcaaata cacacctgca caccgcgccc cggcagcccc gccagcgcct
    13981 cgcgccccga accaaactcg gcaactactt gcaaatcagg ttccagcccc agcaactgcg
    14041 caaagccgga gcggacgatg aggtgatcgt ctataagggc aacggtgatc atgattgggt
    14101 cctggcggat aaaaaacgcg cataccttaa cgataagcgc gatgttgttc aagttggata
    14161 tggggtatgt attggcttaa tgatgttgag cttatcgtca aaacgcttac ctgagaggta
    14221 agcgtttggt gcgtgttatt gagaaaaagc gaattatttg taggttgcaa tcgtaatatt
    14281 gcctgagaaa tttctcctta gattgcggat atcattatcc cttgcccaat aatcatcttt
    14341 gtgtctataa ctctcatcgg aatgtacata gggagaatca tcttcagcgt cgttatggat
    14401 aatattttcg cctttagtgg tatagatata attagcaaca tcaaaggtgt cattcgcgtt
    14461 atttacgact gcgcctaaga cgcaaagggt ttgagtgaat ccgtattctg agaaatcgac
    14521 atgctgatgg taatttctac cattcaaagc aacaaaactc ggacaaacat ctattgtatc
    14581 gccaacagac gtagcggcgc gacgcacatc aagtgcagcg ttatcgttag caacatgaaa
    14641 tgcttgtgaa caggcataaa acaaatttct aatcccggta cgactggata aacaagaaat
    14701 aatttttgct attttttctg gctttatacc atcctgtact aaagaatctc tcaccagatc
    14761 acattcacca ggtgtgaaaa caact
//
LOCUS       DBFIFI010000103        14717 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.103, whole genome shotgun sequence.
ACCESSION   DBFIFI010000103 DBFIFI010000000
VERSION     DBFIFI010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14717
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.103"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            173..1309
                     /locus_tag="N7B39_002972"
                     /pseudo
     CDS             173..1309
                     /locus_tag="N7B39_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            1303..1440
                     /locus_tag="N7B39_002973"
                     /pseudo
     CDS             1303..1440
                     /locus_tag="N7B39_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
     gene            complement(1442..2053)
                     /gene="emtA"
                     /locus_tag="N7B39_002974"
     CDS             complement(1442..2053)
                     /gene="emtA"
                     /locus_tag="N7B39_002974"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HGU2422920.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            2153..3067
                     /gene="ldcA"
                     /locus_tag="N7B39_002975"
     CDS             2153..3067
                     /gene="ldcA"
                     /locus_tag="N7B39_002975"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HGU2422921.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQYDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNTFTEHHFWLALRNETFTIEWQ
                     GEGPTCRAEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILTNTREGTQLTISGHPVLKM"
     gene            3163..4899
                     /gene="cvrA"
                     /locus_tag="N7B39_002976"
     CDS             3163..4899
                     /gene="cvrA"
                     /locus_tag="N7B39_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HGU2422922.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(5288..6358)
                     /gene="dadX"
                     /locus_tag="N7B39_002977"
     CDS             complement(5288..6358)
                     /gene="dadX"
                     /locus_tag="N7B39_002977"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HGU2422923.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEMYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(6368..7666)
                     /gene="dadA"
                     /locus_tag="N7B39_002978"
     CDS             complement(6368..7666)
                     /gene="dadA"
                     /locus_tag="N7B39_002978"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HGU2422924.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            8029..9561
                     /gene="ycgB"
                     /locus_tag="N7B39_002979"
     CDS             8029..9561
                     /gene="ycgB"
                     /locus_tag="N7B39_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HGU2422925.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSVELLERCPPRMG
                     NL"
     gene            complement(9613..10332)
                     /gene="fadR"
                     /locus_tag="N7B39_002980"
     CDS             complement(9613..10332)
                     /gene="fadR"
                     /locus_tag="N7B39_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HGU2422926.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            10554..12095
                     /gene="nhaB"
                     /locus_tag="N7B39_002981"
     CDS             10554..12095
                     /gene="nhaB"
                     /locus_tag="N7B39_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HGU2422927.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            12241..12771
                     /gene="dsbB"
                     /locus_tag="N7B39_002982"
     CDS             12241..12771
                     /gene="dsbB"
                     /locus_tag="N7B39_002982"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HGU2422928.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(12817..14085)
                     /gene="umuC"
                     /locus_tag="N7B39_002983"
     CDS             complement(12817..14085)
                     /gene="umuC"
                     /locus_tag="N7B39_002983"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HGU2422929.1"
                     /translation="MFALCDVNAFYASCETVFRPELWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(14085..14504)
                     /gene="umuD"
                     /locus_tag="N7B39_002984"
     CDS             complement(14085..14504)
                     /gene="umuD"
                     /locus_tag="N7B39_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HGU2422930.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
BASE COUNT         3458 a         3837 c         3683 g         3739 t
ORIGIN      
        1 atatattaat ttctgtgttt ctgattttgc ccggtaatag agaattattc tcagagaagc
       61 tcatcatgat tgcaccatac aaatatcatg atgataagag gatattattc attgacttaa
      121 acaaaatgac aaaatccata gacccatagc ctgggtacta tataggagtg gtatgggcat
      181 caaacaacac aatgggaata ccaaagccga tcgtctcgct gaattaaaaa tccgttcgcc
      241 ctcaattcaa ctgataaaat ttggcgctat tggtttgaat gcaattctct tttcccccct
      301 gctgatagct gctgatacag gaagtcaata tggcaccaat attactatta atgatggtga
      361 cagaattact ggagataccg ccgatccatc aggaaacctc tatggtgtaa tgaccccagc
      421 aggaaacacg cctggcaata tcaacctggg taatgatgtc accgtcaatg tcaacgacgc
      481 ctctggatat gcaaaaggaa tcattattca gggcaaaaac agctccctga cagctaaccg
      541 actcacagta gatgttgttg gtcaaacctc tgccatcggc attaatttaa ttggtgacta
      601 tacccatgct gacttaggca caggcagcac cattaagagt aacgatgacg gcatcattat
      661 tgggcatagc tcaacactaa cagccactca attcaccatt gaaaactcga acggtatagg
      721 cctaaccatc aatgactatg gcaccagtgt cgatcttgga agcggaagta aaatcaagac
      781 cgatggaagt acaggtgttt atatcggtgg tctcaacggc aataacgcca atggtgctgc
      841 gcgttttacg gcgacagacc tgacaatcga tgttcagggc tacagcgcca tggggataaa
      901 cgtacagaaa aactctgttg tcgatctcgg aacaaacagt tccattaaaa ccagtggcga
      961 taatgcacac ggcctctgga gctttggcca ggttagcgcg aatgcactca ctgttgatgt
     1021 aactggagcc gcggccaatg gcgtcgaagt tcgtggtggt acaaccacta tcggtgcaga
     1081 tagccatatt tcttccgcgc agggcggtgg tctcgtcacc agtggttcag acgcgacaat
     1141 caatttttct ggcacggcag cgcaacgaaa cagcatcttt tccggcggtt cttatggtgc
     1201 ctcggcccag acggcaacgg ctgttatcaa catgcaaaat accgatatta cggttgatcg
     1261 taatggcagt ctggcgctgg gtttgtgggc gctcagcggc gcaagaatga aaccatcacc
     1321 actccccgtc tgagcttccg ttttcttaac gtcagcccga cggtggagcg gcaattacag
     1381 cggattattt tctctctcga gcgggaagcc cgggagaaag cggacaaagt acgcgactga
     1441 tttacatcgc gtccagtgcc tgctcaagtt tgtagatata gcgcggagcc tgcggcgcag
     1501 gatgatttcg cgctacgtga tcgaggaact cgtcagcatc cagatcgttg attttgctga
     1561 ttgccttttt ccgatctgac gagaaagtcc gtagcagcgc acctgccccg ttagcatatg
     1621 acaccaccag cgcatattgc agtaccttcg gatcttcaat gcctgccagc gggccggttt
     1681 ccagaatatt caggtaagcc gcccccatag aaatattacg ctccggattt ttcagctcgc
     1741 tggtcgtcgg ctcaccactc cagcccatac gccgataaac atcacgtccg gaggttgaag
     1801 cttttaactg catcaaacca atggcattcg atttactcac cgcgttcgga ttaccacccg
     1861 attcgatagc gataatcgcc gtgatcaatt gtggatcgac gccccaggct gcaccggctt
     1921 tctggcttat tggcatccac tgcatcgcac gttgcaccgg aactttcgcg ttccacggcg
     1981 ggttcgtata gtcatgcttt gatgaacaac ccgctaataa cacaatcaaa aaggcaaacc
     2041 atctcaattt cacttcttct atccttatag cctggaatcg ccgttgaggt gacaacggca
     2101 cacctaagcg gcatgatata catttcgtat ctgttgtcag caaggaattg ccatgtctct
     2161 gtttcactta attgccccat cgggttactg cattaaacag cacgccgcgc tgcgtggtat
     2221 ccagcgcctt accgacgcgg ggcatcaagt caataacgtc gaggttattg cccgtcgttg
     2281 tgagcgtttt gcgggtacgg aaacggagcg tctggaagat ctcaattccc ttgccagact
     2341 gactaccccc aacaccatcg tgctggctgt acgcggcggt tacggtgcca gtcgtttact
     2401 ggcagatatt gactggcagg cactggtggc tcgccaacaa tatgacccgt tgctcatatg
     2461 cgggcatagc gattttaccg ccattcagtg cggtcttctg gcgcaaggca atgtcatcac
     2521 ctttagcggc cccatgctgg tagcaaactt tggcgcggat gaactgaaca cctttaccga
     2581 gcaccatttc tggctggcgt tacgcaatga aacctttacc attgaatggc aaggggaagg
     2641 cccgacatgt cgggccgaag gcacgttgtg gggaggcaat cttgcgatgc tgatttcact
     2701 gatcggtaca ccgtggatgc caaaaattga gaacggtatt ctggtgctgg aagatattaa
     2761 cgagcaccct ttccgcgtcg aacgtatgct gttgcagctc tatcatgcgg gaattttgcc
     2821 acgtcagaag gcgattattc tcggtagctt tagcggcagt acgcctaatg attatgacgc
     2881 aggttacaac ctggagtcag tgtacgcatt tttgcgttcc cgcctgtcga ttccactcat
     2941 taccggcctc gattttggtc atgaacaacg cacggttacg ctgccgttgg gcgcacatgc
     3001 cattcttact aacacccggg aaggcactca gttgacgatt agcggtcatc ctgttcttaa
     3061 aatgtaaaaa aaatcacgct tcagggctaa gtaaaacgct gtctctgccg ctaatatggt
     3121 agggtttata taagagacag cgtaatcagg agtaaccgac cgttggatgc cacaacaata
     3181 attagccttt tcatcttagg atccatcctt gttaccagca gtatattact tagttcattt
     3241 tcttctcgtc ttggcattcc tattctggtt atttttctgg cgatcggcat gctggcggga
     3301 gtcgatggcg tcggcggtat cccatttgat aattacccct tcgcctacat ggtaagtaac
     3361 ctggcgctgg cgattatttt gctcgatggc gggatgcgca ctcaggccag ttcctttcgt
     3421 gtggcgttag gaccggcgct gtcgctggcg acgctgggcg tacttatcac ctctggttta
     3481 accggcatga tggcggcgtg gctgtttaat cttgatttga ttgaaggctt attaatcggc
     3541 gctatcgtcg gctccaccga tgctgcagcg gtcttttctc tgctgggtgg taaggggctt
     3601 aacgaacgtg ttggctcgac gctggaaatt gaatccggca gtaatgatcc aatggcggtc
     3661 tttctgacga ttaccctgat tgcgatgatc cagcagcatg aaagcagtgt cagttggatg
     3721 ttcgtggtcg atattctgca gcaatttggc ctcgggattg tcatcggtct tggcggtggt
     3781 tatttactgc tgcaaatgat taatcgcatc gccctgcccg ccggtttata tccattgctg
     3841 gcattaagcg gcggtatttt aatttttgct ttaaccacgg cgctggaagg tagcggtatt
     3901 ctggctgttt atctgtgcgg ttttctgctg ggtaatcggc cgattcgcaa ccgctacggc
     3961 atcctgcaaa atttcgacgg cctcgcctgg ctggcgcaaa tcgccatgtt cctggtgctg
     4021 gggctgttgg ttaacccaag cgatctgctg cctattgcca ttccggcgct cattttgtcc
     4081 gcatggatga ttttcttcgc ccgtcctctt tcggtatttg ccggattgct accattccgt
     4141 ggcttcaatc tgcgtgagcg cgtgtttatc agctgggtgg gattacgcgg cgcggtgccg
     4201 attattctgg ccgtgttccc gatgatggcg gggctggaga atgcacgtct gttctttaat
     4261 gtcgccttct ttgtggttct ggtttcactg ctcttgcagg gaacatcact ctcgtgggcg
     4321 gcgaaaaaag ccaaagtggt ggttccgccc gtcggacgtc cggtgtcacg cgttggcctg
     4381 gatattcatc cggaaaatcc gtgggagcag tttgtttatc aattgagtgc cgataaatgg
     4441 tgcgtgggcg cggcactgcg cgatttgcat atgccaaaag agacgcgtat tgcggcactg
     4501 tttcgtgata accagttgct tcatcctacc ggcagcaccc gactgcgcga aggcgatgtg
     4561 ttgtgtgtaa ttggtcggga acgcgatctc ccggcgctcg gtaaactgtt cagccagtcg
     4621 ccgccggtcg cgctggatca acgcttcttt ggtgacttca ttctcgaagc cagcgccaaa
     4681 tatgcagatg tggcgctgat atatggcctg gaagacgggc gagaatatcg tgataagcaa
     4741 caaacgctgg gtgaaattgt ccagcagttg ttaggcgcgg caccggttgt cggtgaccag
     4801 gtagagtttg ccgggatgat ctggacggtg gccgagaaag aagataatga agtgttgaag
     4861 attggcgttc gagtggctga agaggaagct gaatcttaaa tttaatgaag catctatcgc
     4921 cggttacgat gctttgctta acgcagcctg agcctgtata taaacttgtc atttcctgat
     4981 tttgatatgc acattccaac atcagaagaa gcctgttaat caacattctg aacatgaacc
     5041 cgaatctgca tcaagggtga agcgtgattt atctgaagtc gagttcgagt caacaatgaa
     5101 gcgtaagcgt tttatgtaga acgcctgtgc ggtgagttca ggttccgggg aaatagtgtc
     5161 catcctttta atgggcacta tcatgggcaa tggctctgtc acattaacaa ctacagttgc
     5221 tgaccagccg gatggatgat tttttttgca accagaagac gttgcctccg atccgactta
     5281 caacaagtta caccgtcaca accgggacgc gtaacgccag cgcgcacatc aactcatagc
     5341 ccaccgttcc ggcagcggcg gcgacatcat caattttgat ctccttgccc cacagctcaa
     5401 ctggcgtacc aatacccgcc tgcgggcaag gcgttaaatc gaccgccagc atatccatcg
     5461 agactgtccc caccgtcatg gtgcgcacgc cgtccactaa aacaggggta ccggtaggcg
     5521 cgtggcgcgg ataaccgtcg gcgtatcctg cggcaacaat gccgattcgc tgctcatcgc
     5581 gcgcagtata acgaccgccg tagcccacac gctcgcccgc ttttagcgtc tggacaccaa
     5641 taatctcact gcttagcgtc atcaccggac gtaatccagt attggcgata tcacgccact
     5701 gaccggacgg cgaagcgcca tacaaaataa tgccaggccg aacccagtca aaatgcgctt
     5761 ctgggtgcca cagagtcgcc gccgaattgg acaacgaacg ccgacactcc agcccttccg
     5821 ccgcctgctc aatacgcgcc atcgcgccgg aaattccatc aggatgttcc gcctcggcaa
     5881 aatgcgacat cagggtcatt tcgccaacat tcgccatcgc ccgcaactgc tgccagacgg
     5941 taagcacgcg atcgggctgg aagcccaacc gattcatccc actgttcact ttaagataaa
     6001 tatccaacgg tgcttttagc cgcgcatttt gcagtgcttt gagctgccag ttgctgtgta
     6061 cgcaggtggt caggcggtgc tggtcataca tttccagatc ctgagcatgg aaaaatcctt
     6121 ccagcatcag gatcggtcct ttccagccgc gctcacgtaa cgttattgct tcttccaggt
     6181 taagtaatgc aaagccatcg gtagccccga gcgcgctcca gatacgctca ataccgtgcc
     6241 cgtaagcgtt cgcttttacc accgaccaga cgcgcgcgtg cggcgcggcc tggcggacaa
     6301 tggacagatt ctgttttaat gcctgcagat cgaggctggc ctgtatcgga cgggtcatct
     6361 cgtttcctta gctgtgtgcg ccatgtaaat ggcccggacg tgatggcgta aatccacggc
     6421 tgtagcgcgc tacgcttaga tcctcatatg ggatcgctgg cgtgcgacca gagagcagat
     6481 cgcttaacaa ctgaccggaa ccgcaagcca tcgtccagcc gagcgtgccg tgaccggtat
     6541 ttagccacag atttttaaag cgtgtacgcc cgacaaccgg cgtgccgtct ggcgtcatcg
     6601 ggcgcagacc cgtccagaaa gtcgcctgct cgacatgacc gccgcgtgga tagagatcgc
     6661 gaaccaccat ctccagcgtt tcacgacgcg gttgcaacag ctcggtatta aaaccaacaa
     6721 tttccgccat tccgccaaca cgaatgcggt tatcgaaacg ggtaatggcg attttgtagg
     6781 tttcatcaag aatggtggat accggcgcac catcttcctg cgcaattgga atggtcagcg
     6841 agtagccttt cagcggatac accggaatat caacaatgcc tttgagcatc gccgtcgagt
     6901 aagaaccaaa cgccatcaca tacgcatcgg ccttaatcac ttcatcgcca cacttcacgc
     6961 cgtagatttg ctcgccgtcg caaagcagtt ggtcaacggg cgtattaaag cggaatttaa
     7021 cccccgcctg ctccgccatc cgcgccagat tctgggtaaa cagctggcag tctccggttt
     7081 catcattggg caattgcaga ccacccgtca gtttgtgcgc tacttctgcc agcgcgggct
     7141 ccacttccgc caggcggctg gattccagca gctgatacgg tacgccggca tcttccagca
     7201 cggcgatatc gcgggtcgca ttttcatact gttgttcggt acggaacagt tgcagcgtcc
     7261 caccctgacg cccttcatac tgaatattgg tttcggcgcg caatgctttc aggcaatcac
     7321 ggctgtattc cgccagacgc accatccgcc ctttgttttc catgtagtgg ctggtgtcgc
     7381 agttacgtaa catttgccac atccatttca actggaactg cgtaccgtcg agacgaaccg
     7441 ccagcggcgc atggcgctgg aacatccatt taatcgcttt taaaggcaca cctggtgccg
     7501 cccacggtgc agcatatccg ggggagattt gcccggcatt cgcggcactg gtttccaggg
     7561 cggcccccgg ctcccgatca atgacggtga cctcatgtcc tgcctgattt aagtaccagg
     7621 cgctggcaac gcctaccaca ccacttccca gtatgacaac tcgcatagcc actccgataa
     7681 cggtagatac agtaaaagaa taataatcta attacatctt gataactcag ttgaaaatat
     7741 tattcaacat acggcttttt tatggtgagc tggctcacat ctccctgact ctgcggcaat
     7801 atggcgtctt tagtatctcc tgctgaacgc cctttttatc cagcataaaa ttccgttcag
     7861 aaacggatta gtggcactct gataataaga aatcgcaaag aaaaaaattc tccggcgacg
     7921 cggtttttaa caggtgttct atgcttgaaa tgaggtgctc cacacagaga gcgccgacaa
     7981 caatgaggtg ctccacacag agagcgccga caacaatgag gtgcgcgtat ggcgacgatc
     8041 gattctatga ataaggacac cacacgtttg agcgatggac ccgactggac gttcgacctg
     8101 ctggatgttt atctggcaga gatagaccgg gtggcgaaac tctaccggct ggatacctac
     8161 ccgcaccaga ttgaagtgat aacctcagaa cagatgatgg atgcctactc cagcgtcggc
     8221 atgccaatta actatccgca ctggtcattc ggtaaaaagt ttatcgagac tgaacggctg
     8281 tataagcacg gtcagcaagg actggcctat gaaatcgtca ttaactctaa cccgtgtatc
     8341 gcttacctga tggaagagaa caccattacc atgcaagcgc tggtgatggc tcatgcctgc
     8401 tatgggcata actctttctt caaaaacaat tacttattcc gtagctggac cgacgccagt
     8461 tcgattgtcg attatctgat ttttgcccgt aaatatatta ccgagtgcga agaacgttat
     8521 ggcgttgatg aagtagaacg gcttctggac tcgtgccacg cgctgatgaa ctacggcgtg
     8581 gaccggtaca aacgcccgca aaaaatctcg ctgcaagaag agaaagcccg gcagaaaagt
     8641 cgcgaagagt atttgcaaag tcaggtcaat atgctctggc gtaccctgcc gaagcgcgag
     8701 gaagagaaaa cggttgctga agcgcgccgt tatccgtccg aaccacaaga aaacctgctc
     8761 tattttatgg agaaaaatgc gccactgctg gaatcatggc agcgtgaaat cctgcgtatt
     8821 gtgcgtaagg tgagccagta tttttatccg caaaaacaga ctcaggtgat gaacgaaggc
     8881 tgggccacct tctggcacta caccatcctt aaccatctgt atgatgaagg gaaagtaacg
     8941 gaacgtttta tgctggagtt tttgcacagc cacaccaatg tggtcttcca gcccccctat
     9001 aacagcccgt ggtacagcgg catcaacccg tatgccctcg ggttcgccat gttccaggat
     9061 attaaacgga tttgtcagtc gccaacggaa gaagacaaat actggttccc ggatatcgcc
     9121 ggttccgact ggctggaaac gctgcatttc gcgatgcgtg atttcaaaga tgagagtttt
     9181 atcagccagt tcctgtcgcc gaaagtgatg cgtgatttcc gcttcttcac cgtgctggat
     9241 gatgatcggc ataattatct ggagatttcc gctattcata atgaagaagg ttatcgggag
     9301 atccgtaacc ggctatcgtc gcaatataac ttaagtaatc tggagccgaa tattcagatc
     9361 tggaacgtgg atttgcgcgg cgaccgttcg ctgacgctgc gttacattcc acataatcgc
     9421 gcaccgctgg atcgggggcg caaagaagta ctgaagcatg tgcatcgcct gtggggattt
     9481 gatgtgatgc tggaacagca aaacgaagac ggcagcgtcg agttgctgga acgttgcccg
     9541 ccgagaatgg gaaatctgta aaaacaacaa aaaacccctc gtttgagggg tttgtttttt
     9601 aaacggaagg gattatcgcc cctgaatggc taaatcaccc ggcagatttt tctgcatccg
     9661 gtgccaaatc tcgccactct catgcccata gcgacgcact gtttcgtaca cctgatcgtg
     9721 cgcgccttca ctgcacaacg ccgacagttt gtggtagaag cccagcgcca gactgcgcgc
     9781 ttccggattg gcgaaatagt gacgaccaat gcgcgtatac agtcccttca tcccgttaag
     9841 gatcagaccg taaatcgggt tgccggaagc aaacgccagg ccgcggaata tgttgtaatc
     9901 cagctcggca aaggcatcgg cgtgatcggc cacttcatta gcggtagcca gcacttcctg
     9961 cgctttatcg ggatgctgac gaaacgcggt gcgaataaaa atagttgaaa tattggtacg
    10021 caccgacagc aaattatcaa taagctgcgg cacactttcg tgatccagtc gcgccagtgt
    10081 ttcaaggata tttaaaccag aggtttccca gaaattattc accttcgtcg gcttgccatg
    10141 ttgaatggtc aaccagccat ctcgtgccag acgctgtaac acttcacgta acgtagtacg
    10201 cgttacgcca attaattctg aaagttcacg ttctgcgggc aaaatagtcc cgggagggaa
    10261 gcggttattc cagatacttt caataatgta ctcttccgcg aaacccgccg ggctttgcgc
    10321 cttaatgacc atagtgagat ttccataaca cagcaaaaca aagttggact catcatacca
    10381 gagggctaac tacgctgata gcagacaaaa agaagaaaaa agggatcaag gcttcatttt
    10441 ccgcatctct ttccccttga ctatcaagac ttgcctcact cctgcctgac cgttaagctt
    10501 tgcgtttaaa cataaaaaca tcattgatta tttgtaaggt aagggaaacc atcatggaga
    10561 tctcctgggg ccgcgcgcta tggcgcaatt ttttgggcca gtcccccgac tggtacaaac
    10621 tcgccctcat cattttctta atcgtaaatc cgttaatttt cctcatcagc cctttcgtcg
    10681 ctggctggtt gctggtagcg gaatttattt tcactctggc gatggccctg aaatgctacc
    10741 cgctgctccc cggtggcctg ttggctatcg aagcggtatt catcggcatg accagcgcgg
    10801 aacacgtccg tgaagaggtg gcggcaaatc ttgaagtctt gctgttactg atgtttatgg
    10861 tggcgggtat ctattttatg aaacagctat tgctgttcat atttacccgt ttgctgttaa
    10921 gcattcgctc caaaatgctg ctgtcgctct ctttttgcgt agcggcagcg ttcctctccg
    10981 cgttcctcga tgccttaacc gtcgtggcgg tggtgatcag cgttgccgtc ggtttttatg
    11041 gtatttatca tcgcgtagcc tcttcccgta ccgaagacac cgacctgcaa gacgatagtc
    11101 atatcgacaa gcattacaaa gtggttctgg aacagttccg tggctttctg cgtagcctga
    11161 tgatgcatgc cggtgtcggc accgcattag gcggcgtaat gaccatggtg ggcgaaccac
    11221 agaacctgat catcgctaaa gcggctggct ggcattttgg cgatttcttc ctgcgcatgt
    11281 cgccggtgac cgttccggtt ctgatttgtg gcctgttaac ctgcctgctg gtagagaagc
    11341 tgcgttggtt tggctacggt gaaacgctgc cggagaaagt ccgcgaagtg ttgcaacagt
    11401 ttgacgatca aagccgccac cagcgtaccc gtcaggataa aatccgtctg attgtccagg
    11461 cgattattgg cgtctggctg gtgactgcgc tggcgttgca tctggcggaa gttggcttga
    11521 ttggtttgtc agtcattatt ctggcaacat cattgaccgg tgtcaccgat gaacatgcta
    11581 tcggtaaagc cttcaccgaa tctctgccat tcaccgcact gttgacggtg tttttctcgg
    11641 tagtcgcggt gattatcgac caacaactgt tttcgccgat tattcagttt gtgttgcagg
    11701 catcggaaca tgctcagctg tcgctgttct atattttcaa cggtctgctg tcatccattt
    11761 cggataacgt cttcgtgggg acgatttata tcaacgaagc gaaagcggca atggaaagtg
    11821 gcgctatcac gttgaagcaa tacgagctgc tggcggtcgc cattaatacc ggtaccaatc
    11881 tgccctctgt cgctacgccg aacggtcagg ctgcgttcct gttcctgctg acctctgcac
    11941 tcgcgccatt gattcgcctc tcttatggcc gcatggtgtg gatggccctg ccttacaccc
    12001 tcgtcctgac actcgtcgga ctgctctgcg tcgagtttac gcttgcccct gtaaccgaat
    12061 ggtttatgca aatgggctgg atagcaacgc tttgataaca acttaccggg catataaatg
    12121 cccggtttgc cttttcgccc gataattgtc caattgcgtc ctatttttgc tgcctcctgg
    12181 tggcgcagcg aatgaattgg tttaaactgc gcactctatg catattgcag ggaaatgatt
    12241 atgttacgat ttttgaacca atgttcacaa ggccggggcg cgtggctgtt gatggcgttt
    12301 actgctctgg cactggaact gacggcgctg tggttccagc atgtgatgtt actgaaacct
    12361 tgcgtgctct gtatttatga acgctgcgca ttattcggcg ttctgggtgc tgcgctgatt
    12421 ggcgcgatcg ccccaaaaac gccactgcgt tatgtagcga tggttatctg gttgtatagt
    12481 gcgttccgcg gtgtgcagtt aacttacgag cacaccatgc ttcagctcta tccttcgccg
    12541 tttgccacct gtgattttat ggttcgtttc ccggaatggc tgccgctgga taaatgggtg
    12601 ccgcaagtgt ttgtcgcctc tggcgattgc gccgagcgtc agtgggattt tttaggtctg
    12661 gaaatgccgc agtggctgct cggtattttt atcgcttacc tgattgtcgc ggtgctggtg
    12721 gtgatttccc agccgtttaa agcgaaaaaa cgtgacctgt tcggtcgcta atccattcgg
    12781 cgctcctgcg ggagcgcttt tttcctgccg ctgtgtttat ttgacccgca gtaaatcaga
    12841 acttcgcgtt gtataacgtg gtgaaagcat ggctcgcttc atctgccatt gttgctggat
    12901 cccctgcccg gcaaaataga gtgttcctct gccctcttta gcattcagtg tatccattac
    12961 cgccatcaat tgctcactcc cggggcgcgg agcgttgtca tcgaataaat tgagctgcgc
    13021 gactccctga ctgaagaaat cccccagcat cacgcccgct ttttggtagc gatggcccgc
    13081 ttgccagatg gcatccagag atcgcgtagc ggcgttaatg atatccctgc tgtcctgagt
    13141 gggcgtcagc agttttaccg acgcgctatt gccgtaataa ggttcattga gcgcaaatgg
    13201 tgacgtctta ataaatgtgg agataaaccg acaatattga tgctcactgc gaagtttttc
    13261 cgccgcccgg gcagcgtaac tacaaatggc ctgccgcatc gacgtataat ccgtgatgcg
    13321 ttcaccaaac gagcgggaac agataatttc ctgctttgtc ggtgcaaatt cttccagttg
    13381 caaacagggt tcaccgcgca gttcacgcac cgttctttcg agcacgacat taaaatgttt
    13441 acggataaac cggatatctg tatccgccaa atcgagaacg gttttgatcc ccatcgcgtc
    13501 cagttttttg ctgatccgcc gtccaatccc ccagacgtca tccacgggga gagcagacat
    13561 taatttacgc tggcgttcca gatttgataa atccaccacc ccacccgtct gccgctgcca
    13621 tttttttgcc gcatgattgg caagcttagc cagcgttttg gtctgggcga tccccacacc
    13681 aacagtaaga tgggtacgtt gtagcaccgt tgcgcgaatt tctctgccaa agtcagtcag
    13741 atcgcgacaa ttacgcacac ctgtcagatc gcagaatgcc tcatcaatac tgtaaatctc
    13801 gacgcggggc gatagttctt ccagcgtcga catcacccga ttgctcatgt ctgcgtaaag
    13861 ctcataattg ctgctaaagc aaaccacgcc acagcgacga aacagatctt tttgtttgaa
    13921 ccagggatcg cccattttaa cgccaagcgc ctttgcctca gcgtttcggg cgataacgca
    13981 accgtcatta ttcgatagca caaccaccgg tttaccccat aactcagggc gaaacaccgt
    14041 ctcacagctg gcataaaacg cgtttacatc acagagggca aacatcagcg catcgcctta
    14101 acgacgtgga tcaccacacc aaagacatcc agcgtatctt cactactgat ggtaatgggc
    14161 gagtacgcgc tgttcatggg aataagctgt accgtcgggc gtagttgcaa ttttttcacc
    14221 gtaaactcgc cgtcaacagc agcgatgaca atatcaccat ggctggcggt aatagcgcta
    14281 tcgacaatca gtaaatcacc gtcactaatt ccaccatcaa tcatagaatc accacttgct
    14341 ttgacgaagt aagtcgcgct gggatgctgg atcaacagtt gattcagatc gatgcgctgt
    14401 tcaacgtaat ctgcggccgg tgaaggaaag ccacactgaa caagatcgct aaatagcgga
    14461 aaagtcacaa tttcgcggag atccgcaggc ttgataaaca acataataat ctgcctgaag
    14521 ttatactgtt tttatataca gtagtctgtt cttgccagca gatcaatact gattcaggct
    14581 atcaatattt gtcgctgcat agactgctga tttttcgttc tcttatcctg tgctcacgcg
    14641 gccttctggc gacgacgctc atccagcaga aatgaaaaat atcacccggc taaaaaatag
    14701 aatagaagca ttcgcca
//
LOCUS       DBFIFI010000104        14324 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.104, whole genome shotgun sequence.
ACCESSION   DBFIFI010000104 DBFIFI010000000
VERSION     DBFIFI010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.104"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            135..653
                     /gene="yfiR"
                     /locus_tag="N7B39_002985"
     CDS             135..653
                     /gene="yfiR"
                     /locus_tag="N7B39_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HGU2422931.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            643..1869
                     /gene="dgcN"
                     /locus_tag="N7B39_002986"
     CDS             643..1869
                     /gene="dgcN"
                     /locus_tag="N7B39_002986"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HGU2422932.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLP
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSAA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYGVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIAHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            1885..2367
                     /gene="yfiB"
                     /locus_tag="N7B39_002987"
     CDS             1885..2367
                     /gene="yfiB"
                     /locus_tag="N7B39_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HGU2422933.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITPP
                     "
     gene            complement(2444..2791)
                     /gene="rplS"
                     /locus_tag="N7B39_002988"
     CDS             complement(2444..2791)
                     /gene="rplS"
                     /locus_tag="N7B39_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HGU2422934.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(2833..3600)
                     /gene="trmD"
                     /locus_tag="N7B39_002989"
     CDS             complement(2833..3600)
                     /gene="trmD"
                     /locus_tag="N7B39_002989"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HGU2422935.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(3631..4179)
                     /gene="rimM"
                     /locus_tag="N7B39_002990"
     CDS             complement(3631..4179)
                     /gene="rimM"
                     /locus_tag="N7B39_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HGU2422936.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(4198..4446)
                     /gene="rpsP"
                     /locus_tag="N7B39_002991"
     CDS             complement(4198..4446)
                     /gene="rpsP"
                     /locus_tag="N7B39_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HGU2422937.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(4583..5944)
                     /gene="ffh"
                     /locus_tag="N7B39_002992"
     CDS             complement(4583..5944)
                     /gene="ffh"
                     /locus_tag="N7B39_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HGU2422938.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            6111..6902
                     /gene="ypjD"
                     /locus_tag="N7B39_002993"
     CDS             6111..6902
                     /gene="ypjD"
                     /locus_tag="N7B39_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HGU2422939.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            6924..8209
                     /gene="yfjD"
                     /locus_tag="N7B39_002994"
                     /pseudo
     CDS             6924..8209
                     /gene="yfjD"
                     /locus_tag="N7B39_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
     gene            complement(8264..8857)
                     /gene="grpE"
                     /locus_tag="N7B39_002995"
     CDS             complement(8264..8857)
                     /gene="grpE"
                     /locus_tag="N7B39_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HGU2422940.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKVKA"
     gene            8980..9858
                     /gene="nadK"
                     /locus_tag="N7B39_002996"
     CDS             8980..9858
                     /gene="nadK"
                     /locus_tag="N7B39_002996"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HGU2422941.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGATRTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            9944..11605
                     /gene="recN"
                     /locus_tag="N7B39_002997"
     CDS             9944..11605
                     /gene="recN"
                     /locus_tag="N7B39_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HGU2422942.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IVEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPETLPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQHYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKTRLQELARLLGGSEVTRNTLANAKELLAA"
     gene            11754..12095
                     /gene="bamE"
                     /locus_tag="N7B39_002998"
     CDS             11754..12095
                     /gene="bamE"
                     /locus_tag="N7B39_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HGU2422943.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(12157..12447)
                     /gene="yfjF"
                     /locus_tag="N7B39_002999"
     CDS             complement(12157..12447)
                     /gene="yfjF"
                     /locus_tag="N7B39_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HGU2422944.1"
                     /translation="MPGKIAVEVAYALPKKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTENKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(12437..12874)
                     /gene="ratA"
                     /locus_tag="N7B39_003000"
     CDS             complement(12437..12874)
                     /gene="ratA"
                     /locus_tag="N7B39_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HGU2422945.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            13045..13527
                     /gene="smpB"
                     /locus_tag="N7B39_003001"
     CDS             13045..13527
                     /gene="smpB"
                     /locus_tag="N7B39_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HGU2422946.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            13742..14104
                     /gene="ssrA"
                     /locus_tag="N7B39_003002"
     tmRNA           13742..14104
                     /gene="ssrA"
                     /locus_tag="N7B39_003002"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         3620 a         3780 c         3366 g         3558 t
ORIGIN      
        1 taaattaaac ataagatttt actcattatg ttttatttct cacaatgatg acgaccgcat
       61 gaaataatga tattttaatg agcattctga aaaaatatcg atgcatttcg agcgaagatg
      121 gtggggatcc ctgaatgcgt ttttctcacc gactgttctt actgctgatt ctcctgctga
      181 cgggagcacc aatactcgct caggagcctt ctgacgttgc gaaaaacgtg cgtatgatgg
      241 tttccggtat tgtcagttac acccgctggc ctgcgctatc agggccacca aagttatgta
      301 tcttttcatc ttcacgtttt agtaccgccc tacaagagaa tgccgcaaca tctttgcctt
      361 atctgcctgt catcattcac actcaacaag aggcgatgat ttcaggctgc aatggttttt
      421 attttggcaa cgagtcaccg acattccaga tggaattaac cgaacaatat ccgtcaaagg
      481 cgttattatt aattgccgaa caaaataccg agtgcattat tggtagcgcc ttttgtctga
      541 tcatccacaa taatgacgtc agatttgccg taaacctgga tgccttatcg cgtagcggtg
      601 taaaggtcaa cccggatgtc ttaatgctcg cacggaagaa aaatgatgga taacgataat
      661 tctcttaata agcgccccac gtttaaaaga gcattacgca acatcagtat gaccagcata
      721 tttatcacta tgatgctgat ctggttgctg ctttccgtga cctcggtgct gcccctgaaa
      781 cagtacgcgc aaaaaaacct ggcactgaca gcagcaacaa tgacttacag tctggaagca
      841 gctgtcgttt ttgccgatgg ccctgcagca actgaaacac tggcagcgct gggccagcaa
      901 gggcaatttt caactgcaga agtacgtgat aagcagcaaa atattctggc atcctggcat
      961 tacacccgta aggatccagg cgatactttc agcaatttca taagccactg gctcttccct
     1021 gcccccatca ttcagccgat tcgtcacaat ggtgaaacca ttggcgaagt acgcttaacc
     1081 gctcgcgaca gttcaatcag ccattttatc tggttttcgc tcgccgtact gaccggttgt
     1141 attctgctgg catcaggaat cgcaattacc ctcacccgcc atttgcacaa tggcctggtg
     1201 gaagcactga aaaatatcac cgatgtcgta catgatgtgc gttccaaccg caatttttcc
     1261 cgacgagttt cggaagaacg tatcgctgag tttcaccgct tcgctctcga cttcaacagt
     1321 ctgctggatg aaatggaaga gtggcagctt cgtttacagg ccaaaaatgc gcagcttcta
     1381 cgtaccgcgc tacatgaccc attaaccggg ctggctaacc gcgcagcgtt tcgtagcggc
     1441 atcaatacgt tgatgaacaa ttccgctgcc cgaaaaacgt cggcgttact atttcttgat
     1501 ggcgataatt tcaaatatat caatgatacc tggggtcatg cgacgggcga tagagtcttg
     1561 attgaaatcg caaaacggtt agctgaattt ggcgggctgc gacataaagc ataccgcctg
     1621 ggcggcgatg aattcgctat ggtgctctat ggtgtacagt cggaatctga agtgcagcag
     1681 atatgctcag cactgacaca aatctttaat ctcccgtttg atcttcataa tggccatcag
     1741 accaccatga cattaagcat tggttacgca atgaccattg cgcacgcttc tgcggaaaaa
     1801 ttacaagagc tggccgatca caatatgtat caggccaaac accagcgtgc cgaaaagctg
     1861 gtgagataac aaggatatat cgatatgata aagcacctgg tagcacccct gattttcacc
     1921 tcactaatac tgactggctg ccagtcccct cagggaaagt ttactcctga gcaagtcgcc
     1981 gctatgcaat cttatggatt tactgaatcc gccggcgact ggtcgctggg cttatcagat
     2041 gccattctgt tcgcaaaaaa tgactacaaa ttgctcccgg aaagccagca acagatccaa
     2101 accatggcag ccaaattggc ctcgacaggg ctaacacatg cccgtatgga tggacacacc
     2161 gataactatg gtgaagacag ttacaacgaa ggcttatcat tgaaacgggc gaatgtcgtg
     2221 gccgatgcat gggctatggg tggacaaatt ccacgcagca atctcaccac acagggttta
     2281 ggaaaaaaat atcccatagc cagtaacaag accgcccagg gccgcgccga gaaccgccgc
     2341 gtcgcagtgg tgattactcc cccttaaaac acgagcaaac agataaaaaa agggccagcc
     2401 aattggccag cccttcttaa caggatgtcg cttaagcgaa atcttagtta agacgctctt
     2461 tgatacgagc agccttacca gtacgctcac gcaggtagta cagtttagct ttacgaacag
     2521 caccacgacg tttgacagaa atgctgtcaa ctaccggaga gtgagtctgg aagacacgct
     2581 caacgccttc gccgttggaa attttacgaa cagtgaatgc agagtgcaga ccgcggttac
     2641 gaatagcgat aaccacgccc tcgaatgcct gcagacgttt tttggaacct tcaacaaccc
     2701 atactttcac ttccacggta tcacccggac ggaaggaagg tacgtcctgc ttcatctgct
     2761 cttgttcaag ttgcttaata atgttgctca taatttaatc tcttatcctg ggtaaactga
     2821 tatctcgggg gcttacgcca tcccatcatg tttatgttgc tgttgtgcgt gttccgtttt
     2881 gaactccgcc agcaaccttg cttgctcttc agtcagagcc aggttttcca gaagttcagg
     2941 tcttctaagc caggtacggc ccagcgactg tttcaaacgc cagcgacgta tctcggcatg
     3001 gttgcccgac agtaacactg gcggaacttc catcccttct aacacctcag gccgcgtata
     3061 gtgcgggcaa tccagcaatc cttcagcaaa ggaatcttcc gttgccgagg cttcatgtcc
     3121 cagtactccc ggaataaacc gggaaacgga gtcaatcagc gtcattgctg gtaactcacc
     3181 accactgaga acgtaatcgc cgattgacca ttcttcgtca atttcggttt ggatcacgcg
     3241 ctcatctata ccttcgtagc gaccgcacac cagaatcaat ttttgattcg ttgccagttc
     3301 gctgacgccc gcttgatcaa gcttgcgtcc ctgtggtgaa agataaatca cctttgcgcc
     3361 ttcacccgcc gcggcttttg ctgcatgaat ggcgtcccgc aagggttgca ccatcattaa
     3421 catccccggt ccgccgccgt aaggacgatc gtccacggta cggtgccggt catgcgtgaa
     3481 atcgcgagga ctccagctct ggatgctcag caggccattt ttaactgccc ggccagttac
     3541 cccgtaatcg gtaattgcgc ggaacatttc aggaaacagg ctaattatgc caatccacat
     3601 agcgccgtct tttaccgttt atccggtggt ttaaaaacca ggatcccaat ctacttcgat
     3661 tgaacgagta gtgagatcga ctttcttgat aacctgccca tcgaggaacg gtacgagacg
     3721 ttccttgata ccaaacgcat ctttcaggtt tgccttaatg acgagaacgt cattggatcc
     3781 ggtttccatc atatcgacga ctttaccgag atcgtagcct tcagtggtta ctacctggca
     3841 gcccatcagg tctttccagt agtagtcacc ctcttcaagc tgaggcagct gcgatgaatc
     3901 cacgacaatt tcacaattcg tcagcaggtt cgccgcatca cgatcgtcaa cgcctttcag
     3961 cttgatgatc atgtcctgat tgtggtgctt ccagctttcc agctggactt gctgccactg
     4021 acccgccttc tggataaacc agggctgata gtcaaaaatg ctttcggcgt cttcggtgga
     4081 agaaaacact ctgagccacc cacgaatacc gtaagacgaa cccatttttc ccaaaacgat
     4141 gggatcaaca ggtgcttgcg cggtgagttg tttgctcatc atgaccaccg tgacagatta
     4201 agctgctttg tttacttctt tgatcagcgc agcaacgcga tcagaaatag ttgcgccctg
     4261 gccaacccag tgagcgatgc gatccagatc caggcgagtg ccttcttctt tttcgctagc
     4321 gattgggttg aagaaaccaa cgcgctcgat gaagcgaccg ttgcgtgcat tacggctgtc
     4381 agcgacaaca acctggtaga acggacgctt tttagcgccg tgacgtgcta aacgaatagt
     4441 taccataaca tcctcttgtg tgaataaaac aaccggaccc catcgaggaa cggagtccgg
     4501 tgtcatatta aaagcccgaa aattttactg atttctggag aaaatgcaat cagcagttaa
     4561 taactctgtc gcataaggtc atttagcgac caggaaatcc cggcggcatc atccccttca
     4621 tgcttctcat catcttcgcc attccgccct tcttcatttt cttcatcatg cgctgcatgt
     4681 cgtcgaactg tttcagaaga cggttaacgt cctgcacctg cataccgcaa ccggcagcaa
     4741 tacggcgttt acgcgaacct ttgatgattt ctggcttagc gcgctctttc atcgtcatcg
     4801 agttgatgat ggcttccata cgcaccagca ctttatcgtc catctgtgac ttgacgttat
     4861 ccgggatctg ccccatgccc ggcagtttgc ccatcaggct ggccatgccg cccatatttt
     4921 tcatctggcg cagttgctca aggaagtcgt tgagatcgaa gccgtcacct tttttcagct
     4981 tgctggctaa tttctctgcc tgcgcgcggt caactttgct ttcgatatct tcgatcagcg
     5041 acagtacgtc gcccatgccg agaatacgcg acgcgatgcg gtccggatgg aacggctcca
     5101 gcgcctcagt tttctcgcca acaccgagga acttgatcgg tttgccagtg atgtgacgaa
     5161 tagagagcgc cgcaccgccg cgggcatcgc cgtccacttt ggtcaacact acgccggtaa
     5221 gcggtaacgc ttcattgaat gcttttgccg tattggccgc atcctgaccg gtcatggcgt
     5281 caaccacaaa cagggtttca accgggttaa tcgacgcatg gacttgtttg atctcgtcca
     5341 tcatcgcttc gtcaacgtgc agacgaccag cggtatccac cagcagcacg tcgtagaatt
     5401 tcagtttggc ttctttcagc gccgcgttaa caatatcaac cggcttctga ccaacatcag
     5461 aagggaagaa atcaacgccc acctgctctg ccagcgtctc aagctgtttg attgccgccg
     5521 ggcgatagac gtcagcagaa accaccagca ctttcttctt gtgcttctcg cgcaggaact
     5581 taccgagttt accaacgctg gttgttttac cggcaccttg caggcctgcc atcagcacga
     5641 ccgcaggcgg ttgcgcagcc agattcaggg tctggttctc ttcgcccatc gccgcaacca
     5701 gttcgttacg gacgattttg acgaactcct gccccggcgt caggctctta ttaacttcat
     5761 gaccaaccgc tttctctttt acgcgattga taaactcacg cactaccggc agagcgacgt
     5821 ccgcctccag cagcgccatg cgcacttcgc gcagcgtatc ttttacgttg tcttcagtga
     5881 ggcgtccacg gccactgata ttgcgcagcg tgcgcgacaa acgatcggtt aaattatcaa
     5941 acattgtctc tcgcctgggg tggaaacggt tggccgcaat cgcgacacat catcagtatt
     6001 ttgccgcagt ataacatgaa ggcgtctttg ttgttatgca acggttggag cagcgttcac
     6061 ctgacgctat actgcttctc tttcttattg ctcaaactgt cgacatcact atgcccgttt
     6121 ttgctctgct cgcgcttgtc gcctactccg tcagtcttgc gctgattgtt cccggtctgc
     6181 tgcaaaaaaa cggcggctgg cggcgcatgg ctattatttc tgcggtcatt gcgctggtct
     6241 gccacgcaat cgctctggaa gcccgcatcc tgcccgacgg tgatagcgga caaaacctca
     6301 gcctgctgaa cgttggttca ttggtcagtt tgatgatctg tacggtaatg accattgtgg
     6361 cttctcgcaa tcgtggctgg ttgctgctac ccattgtcta tgcctttgcg cttatcaacc
     6421 tggcgctggc aaccttcatg cccaatgaat acatcaccca tctggaagct acgcctggga
     6481 tgctggtgca cattggctta tcgctctttt cctatgccac gctaattatc gccgccctgt
     6541 acgcgctgca actggcgtgg attgattacc aactgaagaa caagaagctg gcgtttaacc
     6601 aggaaatgcc gccattgatg agtatcgagc gtaaaatgtt ccacatcacg cagattggcg
     6661 tggtgctgct aacgctcacg ctttgcactg gcctgttcta catgcacaac ttatttagca
     6721 tggaaaatat cgacaaggct gtgctctcta tcgtggcgtg gtttgtctat attgtgctgc
     6781 tatggggaca ttatcatgaa ggatggcgtg gacgtcgcgt cgtctggttt aacgttgcgg
     6841 gtgcagtcat tctgacactg gcctacttcg gcagccgaat tgtccagcag ttaatcagct
     6901 aaacccagaa aaggagtttc cccctggaac acatttctac tactacgttg atcattattc
     6961 tgatcatcat ggtggtcatt tcagcctatt tttccgggtc cgaaaccgga atgatgaccc
     7021 tcaaccgcta tcgtctgcga catatggcga aacagggtaa tcgctcggcc aaacgcgtcg
     7081 aaaaattgct gcgtaagcca gaccgcctga taagcctggt gttaatcggc aataacctgg
     7141 tcaatattct tgcctccgcg ctcggcacta ttgttgggat gcgtttgtac ggcgatgcgg
     7201 gcgtggcaat tgcgactggt gtgctgactt ttgtcgtact ggtttttgct gaggtattgc
     7261 cgaaaaccat tgccgcgctg tacccggaaa aagtcgctta tccgagtagt tttctgctgg
     7321 ctcccctgca aattttgatg atgccgctgg tctggttgct gaatgctatc acccgtatgc
     7381 tgatgcgcat gatgggtatc aaaaccgata tcgtggttag cggctctttg agcaaagaag
     7441 agttgcgcac tatcgtgcac gaatcgcgct cacaaatttc ccgtcgcaat caggatatgc
     7501 tgctgtcggt gctcgatctg gaaaaaatga ccgttgatga catcatggtg ccgccagtga
     7561 aattatcggt attgatatca acgatgactg gaaatctatt ctgcgccaac tctcccactc
     7621 acctcacggg cgcatcgtgc tctaccgtga ttcgctggac gacgccatca gtatgctgcg
     7681 agtacgtgaa gcttggcggt tgatgtcgga gaaaaaagag ttcaccaaag aaaccatgct
     7741 gcgcgccgcg gacgagatct attttgtgcc ggaaggtacg ccgctcagca cgcagttggt
     7801 gaagtttcag cgcaacaaaa agaaagtcgg cctggtcgtc aacgagtatg gagacattca
     7861 ggggctggtg acggttgaag atattctgga agagattgtc ggcgatttca ctacgtcgat
     7921 gtcgccaaca cttgccgaag aagtcacgcc gcaaaacgac ggttcggtga ttattgatgg
     7981 cactgccaac gtgcgggaaa tcaacaaagc ctttaactgg catctaccgg aagatgatgc
     8041 ccgcacggtt aatggcgtca ttcttgaggc actggaagag atccctgtcg taggcacccg
     8101 cgtgcgtatt ggcgagtacg atatcgatat tctcgacgta caggacaata tgattaagca
     8161 ggtaaaagtt tttcctgtga aaccgctgcg cgagagtgtg gcggagtaac gaaaacggcc
     8221 cggcattcgc atgcagggcc gtgaattatt acgaaaacag aaattacgct tttactttcg
     8281 ctacagttac catcgccgca cgaatcgtac gaccattcag cgtataaccc ttctgcataa
     8341 tgcccagtac gttacctggc gcaacgtcat cagattccac cattgcgata gcctgatgca
     8401 cattcgggtc cagcggcacg ttagtttcgg cgatcacttc aacaccaaac ttacgcacaa
     8461 catccagcat cgacttcagc gtcagctcaa tgccttcaac catcgcagac atatccgggt
     8521 tagctttatc agccacttcc agcgcacgat ccaggctatc aatcaccggc agcaattcgt
     8581 tgatgaattt ctccagcgcg aatttgtggg ctttttcaat atccagttca gtacgacgac
     8641 gcaggttttc catttcggct tttacacgca aaatgccgtc acgttcacgg gtctgggctt
     8701 cagccagctg agcttcgaga ttcgcaattt tttcatcgcg cggatccacc tgctcagcag
     8761 aagcttctgg ctcaactgcc tcaatctctt cgtgctgatc catgataatt tcttccgggg
     8821 cttgcccctc aggcgttttc tgttctttac tactcatgaa tttctccgcg tttttttcgc
     8881 attcatctcg ctaacttcgc ttattatggg gatcagtttc agggtttcaa gggaagcact
     8941 cacattgtca tcaatcttcg caacaaggac ctcggaaaaa tgaataatca tttcaagtgt
     9001 attggcattg tgggacaccc acggcaccct actgcactga caacacatga aatgctctac
     9061 cgctggctgt gcacaaaagg ttacgaggtc atcgttgagc aacaaatcgc tcacgaactg
     9121 caactgaaga atgtgaaaac tggcacgctc gcggagattg ggcaactggc tgatctcgcg
     9181 gtagtcgttg gtggcgacgg taatatgctg ggcgcgacgc gcacgctcgc ccgttacgat
     9241 attaaagtta ttggaatcaa ccgtggcaac ctgggtttcc tgactgacct tgaccccgat
     9301 aacgcccagc aacaattagc cgatgtgctg gaaggtcact acatcagcga gaaacgtttt
     9361 ttgctggaag cgcaagtctg tcagcaagat tgccaaaagc gcatcagcac cgcgataaat
     9421 gaagtggtac ttcatccagg caaagtggcg catatgatag agttcgaagt gtatatcgac
     9481 gagatctttg cgttttctca gcgatctgat gggctaatta tttcgacgcc aacaggctcc
     9541 accgcctatt ccctctctgc gggcggtcct attctgaccc cctctctgga tgcgattacc
     9601 ctggtgccca tgttcccgca tacgttgtca gcacgaccac tggtcataaa cagcagcagc
     9661 acgatccgtc tgcgtttttc gcatcgccgt aacgacctgg aaatcagttg cgacagccag
     9721 atagcactac cgattcagga aggtgaagat gtcctgattc gtcgctgtga ttaccatctg
     9781 aatctgattc atccgaaaga ttacagttat ttcaacacat taagcaccaa gctcggctgg
     9841 tcaaaaaaat tattctaatt ttacgccagc ctctttactg tatataaaac cagtttatac
     9901 tgtacacaat aacagtaatg gtttttcata caggaaaacg actatgttgg cacaactgac
     9961 catcagcaac tttgctatcg ttcgtgagct tgagattgat tttcatagcg gcatgaccgt
    10021 aataactggc gaaaccggtg cgggtaaatc tattgcaata gatgccctcg gtctttgtct
    10081 cggtggtcgc gctgaagccg acatggtgcg taccggcgct gctcgcgctg acctgtgcgc
    10141 ccgtttttct ctgaaagata cgccagcggc cctgcgctgg ctggaagaaa accagcttga
    10201 agacgggcat gaatgtttgc ttcgtcgcgt gatcagcagc gatggtcgct cccgtggttt
    10261 catcaacggt acagctgttc ctctgtcaca actgcgcgaa ctgggtcagt tgctgattca
    10321 gatccatggt cagcacgctc atcagttact caccaaaccc gagcaccaaa aattcctgct
    10381 tgatggctat gccaatgaaa cctctctact ccaggaaatg accgcacgtt atcagttgtg
    10441 gcatcaaagc tgccgtgacc tcgcgcatca tcaacagtta agtcaggaac gcgctgcccg
    10501 tgcggaactg ctgcaatacc aattaaaaga acttaacgaa tttaatccgc agcccggaga
    10561 gtttgagcaa atcgacgaag agtacaaacg tctggcgaac agcggtcaat tgctgaccac
    10621 cagccagaat gcattggcat taatggccga cggtgaagac gcaaacctgc aaagtcagct
    10681 ttacacggct aaacaactgg tgagcgaatt gattggcatg gacagcaaac tgtccggcgt
    10741 acttgatatg ctggaagaag ctaccatcca gattgttgaa gccagcgacg aactgcgcca
    10801 ctactgcgat cgtctggatc tcgatcccaa ccgactgttt gaacttgaac agcgcatctc
    10861 aaaacagatt tcgctggcac gtaaacatca cgtcagccct gagacattgc cacagtatta
    10921 ccagtcgcta ctggaagaac agcagcaact ggacgatcag gccgactcac aagaaacgct
    10981 cgcactggcg gtaacgaaac atcatcagca ggcactggaa acggcgcgcg cattacacca
    11041 gcaacgccag cattatgcag aagaacttgc acagctgatc accgacagta tgcatgcgct
    11101 ctcaatgccg catgggcagt ttacgattga tgtcaaattt gacgagcatc acctgggcgc
    11161 tgacggcgcc gatcgtattg agtttcgggt aaccaccaac ccaggtcagc caatgcagcc
    11221 tattgccaaa gtcgcatccg gtggtgaatt gtcccgcatc gcattggcaa ttcaggtcat
    11281 cacggcgcgt aaaatggaaa ccccggcact gatttttgat gaagtggatg tagggattag
    11341 cggtccaaca gcggcagttg tcggcaaact gctgcgtcaa ctcggcgaat caactcaggt
    11401 gatgtgtgtt acccacctgc cacaagtcgc gggatgtggt catcaacact attttgtcag
    11461 caaagaaacc gatggtgcga tgacagaaac gcatatgcaa tccctgaata aaaaaacgcg
    11521 gttacaagag ctggcgcgcc tgcttggtgg cagtgaagtc acacgtaaca cactggcgaa
    11581 tgcgaaagaa ctgcttgcgg cgtaaacttt tttcctgctt cacggtcaga gtaaacagca
    11641 aaacgccgta agaccggaaa gcaaaaggtt ttaaagtgat gaaaggtcta ttatcatcgg
    11701 catattacag atgagccacg tactgctcgg gcccgaaaag gaatcaaatc actatgcgct
    11761 gtaaaacgct gactgctgca gcagcagtac tattgatgtt gaccgcaggc tgttccactc
    11821 tggagcgagt ggtttaccgt cctgacatca accaggggaa ctatctgacc gctaacgacg
    11881 tatccaaaat acgtgttggc atgacgcaac aacaagttgc gtacgcattg ggtacaccgc
    11941 tgatgtccga tccatttggt acgaatacct ggttctatgt cttccgccag caaccaggtc
    12001 atgaaggtgt aacgcagcaa acgctgacgc taacctttaa cagtagcggt gtgttgacca
    12061 atattgataa caaacctgcg ctgagtggta actaataata aagttgctct caaagacgtt
    12121 aaaaaaggtg ctcaatgagc accttttttc tgtccgttat ttattcgctg atttttctgc
    12181 tcgttgcctg cgaagctctt tcggatcggc aatgagaggg cgataaatct ccacccgatc
    12241 gccatcatgc acactatcgc ttaattttgc cggacggctg taaatgccga ctttgttttc
    12301 agttaaatcg atatcggtac gcaattccag caagccactg gcgcgaatag cttcttcaac
    12361 cgtcgcgccc tcctgcagcg tcacgcgctg caggtactgc ttcttaggta gcgcataagc
    12421 cacctcaacg gcaattttac ctggcactgt aaacctcttt cgcacgaacc gtaaaagcct
    12481 ggaccatatt agacgccagc tctttaaaca cgcgaccaaa agcgagttca atcaacttat
    12541 tggtaaactc aaagtcgaga tgaaactcga tacgacacgc ctcctggctc agcggcgtaa
    12601 acttccatcc accaatcaat ttcttgaacg gcccatccac cagattcata agaatacttt
    12661 ggttactggt caactggttg cgggtagtaa acgttttgct gatcccagcc ttagagacat
    12721 ctaccgccgc agtcatctgc ccaggagtgg actccagaat ccgacttccg gtacaacccg
    12781 gcaaaaactg aggataagac tgaacgtcat tcactaactg atacatttgc tccgcgctgt
    12841 agggtaccag tgcggtccgg ctaatctgag gcataacaat ttccatcaac aaaaatccaa
    12901 caaataatat catttatcct gcagtaaaaa aaacgctatc ccggcgctgg gtaacatccg
    12961 gttcatgcta agatagagcc ttgtcccccg caggattgat atggggtatt ttcgatttca
    13021 gattaccgat gattcacgac gcttatgacg aagaaaaaag cacataaacc tggttcagcg
    13081 accatcgcgc ttaacaagcg cgcccgtcac gaatacttta ttgaagaaga gttcgaagcg
    13141 ggacttgccc tgcaaggctg ggaagttaaa tccctgcgcg caggaaaagc caatattagc
    13201 gacagctatg tccttctgcg cgacggagaa gcgttcctgt ttggcgcaaa catcacgcca
    13261 atggctgtgg cttctacgca tgtggtgtgc gatcctaccc gtacccgcaa gttacttctc
    13321 aaccagcgcg aacttgattc gttgtacggt cgcgtcaatc gagaaggcta taccgtagtg
    13381 gcgctctccc tgtactggaa aaatgcctgg tgcaaagtga aaatcggcgt cgccaaaggt
    13441 aagaaacagc acgataaacg ttcagatatc aaagaacgcg aatggcaggt ggataaagcg
    13501 cgtatcatga aaaacgccca ccgttaaacc tgcactccaa ttcttgacct gttcctcacc
    13561 gcgcctccct ctccggcggc gcgaatgaac atcttattgg ctatcacatc cgacaaaaat
    13621 gttgccatcc cattgcttaa tcgaataaaa atcaggctac atgggtgcta aatctttaac
    13681 gataacgcca ttgaggctgg tcatagcgct cataaatctg gtatacttgc cgttacacat
    13741 tggggctgat tctggattcg acgggatttg cgaaacccaa ggtgcatgcc gaggggcggt
    13801 tggcctcgta aaaagccgca aaaaatagtc gcaaacgacg aaaactacgc tttagcagct
    13861 taataacctg cttagagccc tctctcccta gcctccgctc ttaggacggg gatcaagaga
    13921 ggtcaaaccc aaaagagatc gcgtggaagc cctgcctggg gttgaagcgt taaaacttaa
    13981 tcaggctagt ttgttagtgg cgtgtccgtc cgcagctggc aagcgaatgt aaagactgac
    14041 taagcatgta gtaccgagga cgtaggaatt tcggacgcgg gttcaactcc cgccagctcc
    14101 accactttaa gaaggactac aaccggacag tagcaataaa tacagccact tacggacaca
    14161 agccagacag tagtcggact gaaaaaagac aaaaatatac acgtgaaatg cacgtgcact
    14221 tctaaaaaac cccagttatc accgactggg gtttttcaat ttgtaactaa gggtaacaac
    14281 ccccccgttt ttttcatctc actcaccttg atactgttta tttg
//
LOCUS       DBFIFI010000105        14316 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.105, whole genome shotgun sequence.
ACCESSION   DBFIFI010000105 DBFIFI010000000
VERSION     DBFIFI010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14316
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.105"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            154..1725
                     /gene="garD"
                     /locus_tag="N7B39_003003"
     CDS             154..1725
                     /gene="garD"
                     /locus_tag="N7B39_003003"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HGU2422947.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPKAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            1874..2209
                     /gene="prlF"
                     /locus_tag="N7B39_003004"
     CDS             1874..2209
                     /gene="prlF"
                     /locus_tag="N7B39_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HGU2422948.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            2209..2673
                     /gene="yhaV"
                     /locus_tag="N7B39_003005"
     CDS             2209..2673
                     /gene="yhaV"
                     /locus_tag="N7B39_003005"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HGU2422949.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH"
     gene            complement(2728..3537)
                     /gene="agaR"
                     /locus_tag="N7B39_003006"
     CDS             complement(2728..3537)
                     /gene="agaR"
                     /locus_tag="N7B39_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HGU2422950.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            3786..5066
                     /gene="kbaZ"
                     /locus_tag="N7B39_003007"
     CDS             3786..5066
                     /gene="kbaZ"
                     /locus_tag="N7B39_003007"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HGU2422951.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKATALSQMVESYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLNRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            5089..5562
                     /gene="agaV"
                     /locus_tag="N7B39_003008"
     CDS             5089..5562
                     /gene="agaV"
                     /locus_tag="N7B39_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HGU2422952.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGIPTEPAVDLFKLL"
     gene            5573..6352
                     /gene="agaW"
                     /locus_tag="N7B39_003009"
     CDS             5573..6352
                     /gene="agaW"
                     /locus_tag="N7B39_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HGU2422953.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            6342..7220
                     /gene="agaE"
                     /locus_tag="N7B39_003010"
     CDS             6342..7220
                     /gene="agaE"
                     /locus_tag="N7B39_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HGU2422954.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            7238..7672
                     /gene="agaF"
                     /locus_tag="N7B39_003011"
     CDS             7238..7672
                     /gene="agaF"
                     /locus_tag="N7B39_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HGU2422955.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITSTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            7669..8802
                     /gene="nagA"
                     /locus_tag="N7B39_003012"
     CDS             7669..8802
                     /gene="nagA"
                     /locus_tag="N7B39_003012"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HGU2422956.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVSVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLSTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASVVALDSGLHVQQIWIQGQLASF"
     gene            9153..10307
                     /gene="agaS"
                     /locus_tag="N7B39_003013"
     CDS             9153..10307
                     /gene="agaS"
                     /locus_tag="N7B39_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HGU2422957.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALHSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFAVL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELHRDNQAMRVIAIAAESSDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            10320..11180
                     /gene="kbaY"
                     /locus_tag="N7B39_003014"
     CDS             10320..11180
                     /gene="kbaY"
                     /locus_tag="N7B39_003014"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HGU2422958.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            11347..11823
                     /gene="agaB"
                     /locus_tag="N7B39_003015"
     CDS             11347..11823
                     /gene="agaB"
                     /locus_tag="N7B39_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIB"
                     /protein_id="HGU2422959.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDVNVGNMHFSEGKKQISSKVYVDDQDLTDLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            11895..12665
                     /gene="agaC"
                     /locus_tag="N7B39_003016"
     CDS             11895..12665
                     /gene="agaC"
                     /locus_tag="N7B39_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HGU2422960.1"
                     /translation="MAALVFVLGIDFWLEALFLFRPIIVCTLTGAILGDIQTGLITGG
                     LTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLPFSLLMQYVILF
                     FYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCTYLAQGAMQALV
                     KAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVCYIQVSNLLPVA
                     VLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            12655..13446
                     /gene="agaD"
                     /locus_tag="N7B39_003017"
     CDS             12655..13446
                     /gene="agaD"
                     /locus_tag="N7B39_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HGU2422961.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            13447..14202
                     /gene="agaI"
                     /locus_tag="N7B39_003018"
                     /pseudo
     CDS             13447..14202
                     /gene="agaI"
                     /locus_tag="N7B39_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417610.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
BASE COUNT         3458 a         3611 c         3742 g         3505 t
ORIGIN      
        1 tgccgggcgg cattgcaatc cctgaaatga ttgacattga tcacatttct gcgtttaaac
       61 tcctgacatt cttatttcac ccaatgaagt catttatttt taaatgagac caggtcctca
      121 ttttaataac ctctggctgg agaatattgc acaatggcca acatcgaaat cagacaagaa
      181 acgccaactg cgttttatat aaaagttcac gacacagata atgtggcaat tattgttaat
      241 gataatggcc tgaaagcagg aacgcgtttt ccggatgggc tggaattaat tgaacatatt
      301 ccccaggggc ataaagtcgc attgctcgac attccggcta atggtgaaat tattcgttat
      361 ggcgaagtga ttggttacgc cgtgcgtgca atcccacgcg gaagctggat cgacgaatca
      421 atggttgtac tgccgaaagc gccgccgtta cacacgctgc cactggcaac caaagtcccg
      481 gaacccttac cgccgctgga aggatacacc tttgagggct atcgcaatgc cgatggcagc
      541 gtgggcacca aaaacctgct cggcatcacc accagcgtcc actgtgtggc aggcgtggtg
      601 gactacgtag taaaaatcat tgaacgcgat ctgctaccga aatacccgaa cgtcgatggc
      661 gtggtggggc tgaatcattt gtacggttgt ggcgtggcga ttaacgcacc ggcggcagtt
      721 gtacctatcc gtaccattca caatatttcg ctgaatccta actttggcgg cgaagtaatg
      781 gtgattggcc tgggttgtga aaagttgcag cctgagcgcc tgctgactgg aacggatgat
      841 gtgcaagcta ttccagtaga aagcgccagc attgtcagtt tgcaggatga aaagcatgtc
      901 ggttttcagt ccatggtcga ggatattttg caggtcgccg aacgccatct acaaaaactg
      961 aatcaacgtc agcgagaaac ctgtccggct tcagaactgg ttgtcggcat gcagtgcggt
     1021 ggcagcgatg ccttttctgg cgtaacggca aacccggcgg ttgggtatgc gtctgatcta
     1081 ctggtgcgct gcggcgcaac ggtgatgttc tcagaagtca cggaagtgcg tgacgcgatc
     1141 catctgctga caccacgcgc agtgaacgaa gaggtcggca aacggctgct ggaggagatg
     1201 gagtggtacg ataactatct caatatggga aaaaccgacc gcagcgccaa cccttcgccg
     1261 ggcaacaaga aaggcggtct ggcaaatgta gtggagaaag cgctcggctc cattgctaaa
     1321 tcgggtaaaa gtgcaattgt tgaagtgctg tcgcccggtc aacgcccgac taaacgcgga
     1381 ttaatttacg ccgcgacgcc agccagcgat tttgtctgtg gcacgcaaca ggtggcttcg
     1441 ggtatcaccg tgcaagtgtt tacgaccggt cgtggtacgc cgtacggcct gatggcggta
     1501 cccgtcatta aaatggcgac ccgcaccgag ctggcgaacc gctggtttga tttaatggat
     1561 attaacgcgg gcactatcgc caccggcgaa gaaaccattg aagaggtggg ctggaagttg
     1621 ttccacttta ttctcgacgt cgccagcggg aagaagaaaa ccttctcgga tcaatgggga
     1681 ctgcataacc agctggcggt gtttaaccca gcaccggtga cctgatttct tttggaacgt
     1741 ccctcgcaaa acatggcctt agtgccatgt ttttattgtt taaagccccc acgtccatta
     1801 ataatgcatt tgcattacct tagttcaagc ttataatttg agcagaaaac aggctgtaaa
     1861 aggacagtga atcatgcccg ctaatgctcg ctctcacgct gtactgacca ctgaatcaaa
     1921 ggtcacgata cgcggacaaa caactatccc cgcgccagtg cgtgaggcct taaaactgaa
     1981 gccaggccag gacagcattc attacgaaat tctgcctggt gggcaagtat ttatgtgccg
     2041 actgggagat gagcaggagg atcatactat gaatgcattt ttgcgttttc tggatacaga
     2101 tatccagaac aacccgcaaa aaactcgtcc attcaacatt caacaaggaa agaaacttgt
     2161 cgctggcatg gacgtcaaca ttgatgatga gattggcgac gacgaataat ggattttcca
     2221 caaagggtta atggttgggc gctatatgct catccctgtt ttcaggaaac ctacgacgct
     2281 ttagttgccg aagtcgagac attaaaggga aaagatcctg aaaattatca gagaaaagct
     2341 gccacaaagt tattggcggt ggtccataaa gtgattgagg agcatatcac ggtcaatcca
     2401 tcatcaccgg cattccgtca tggcaagtcg ttaggctctg ggaaaaataa agactggtca
     2461 cgggtaaaat ttggtgctgg tcgctatcgt ctcttctttc gttacagtga aaaagagaaa
     2521 gtcatcattc tgggatggat gaacgatgaa aacacgctgc gcacctacgg taaaaaaaca
     2581 gatgcctata ccgtattcag caaaatgtta aaaagaggac atcctcctgc cgactgggaa
     2641 accctcaccc gagagacaga agaaacccat tgatggtgtt cacaatgccc gccgaacgca
     2701 ttcgtgttga ccagcgggca ccctttctca ctccccgacc agaatcactt caaccccagc
     2761 ctttcgcagc ccttccaggc tatccgcagg aatgccttca tcaacaatga tcatgtcgat
     2821 acgttgagta tcaatgatct tatgtaaact ggaacgattg aatttactgg aatcggtgac
     2881 cacgatgatc cgttccgcaa cttcgcacat ccggcggttt aaacgggctt catcttcatt
     2941 atgcgtgctg acgccgcgct ccagatcgat cgcatctaca ccaagaaaca gcatatcgaa
     3001 gtggtaattt tgcagcgatt gctcagcctg atcgccgtaa aaagattgcg actgacggcg
     3061 caaatgcccg ccggtcatca gcagctcaac gccttccgct tccagcaacg cattagccac
     3121 gttcataccg ttggtcatcg caattacgtc agtgtgcttg cgcatcagac gagcaatctc
     3181 aaaagtggtg gtcccggaat cgaggatcac ccgatgacct ggctgaatca actcaacggc
     3241 agctttcgca acgctgcgtt tcattgcggt gttcagcgcg cttttatctt ccactgatgg
     3301 ctcgactgac ggcgttgtgc tatcgcagat caacgcgcca ccataggcac gcacagcgat
     3361 cccctgcttt tccagaaacg ccagatcgtt gcggatcgtc acggtagata cgccatacaa
     3421 tgccgacaga tcgttaacct gcacactccc ttgctgtcgc agacgctgaa tgatctgttc
     3481 tcgtcgctcg ctggtgcctg tcactcgctt ctcacctgaa gcgtcggtat tactcatagt
     3541 aagtcctttc gtaaaacttt cgtttcattt cgttttgcct attaacgcct ttctattaag
     3601 caaatgcaag cccaccttgc ccattgacgc aagctactct cgtttcagtg actttcatta
     3661 tgtttctttt gtgaatcaga tcagaaaact attatctttc gttttatttt tatctcacca
     3721 tgacgcagta tcaactgaaa caaaacgaaa gattaatatc gcagtaatct gaactggaga
     3781 ggaaagtgaa acatctgaca gaaatggtga gacagcacaa agcgggcaaa acaaatggaa
     3841 tttatgccgt ttgttccgca catccgctgg tgctggaggc tgcaatccgc tacgccagtg
     3901 caaaccaaac gccgttactg attgaagcaa cctccaatca ggtagaccag ttcggcggtt
     3961 ataccggaat gacgcccgcc gattttcgcg gctttgtttg tcagctcgcc gactcgttga
     4021 atttcccgca ggatgcgttg attctgggtg gtgaccatct ggggccaaac cgctggcaaa
     4081 acctgccggc cgctcaggca atggccaatg ccgatgattt gattaaaagc tacgttgcgg
     4141 caggattcaa aaaaatccac cttgattgca gcatgtcctg tcaggacgat ccgattccct
     4201 taactgatga catcgtggct gaacgcgccg cccgtctggc gaaagtggcg gaagaaacct
     4261 gtcttgaaca ctttggcgaa gccgatctgg agtatgtcat tggtaccgaa gtgccggtac
     4321 ctggcggcgc gcatgaaacc ttaagcgagc tggcggtcac cacgccggat gccgcccgcg
     4381 ccacgctgga agcccatcgt cacgcctttg aaaagcaagg tttgaatgcc atctggccac
     4441 gcatcattgc cctggtggtt caacccggcg tcgaattcga tcacaccaac gttattgatt
     4501 atcagcccgc caaagcgacc gccttaagcc agatggtcga aagctacgaa acgctgattt
     4561 tcgaagcgca ctctaccgat tatcaaacgc cgcaatcgct gcgccagctg gtgattgacc
     4621 actttgccat tctgaaagtt ggcccggcgc tgaccttcgc cctgcgtgaa gctctgttct
     4681 ctctggcagc gattgaagaa gaactggtgc cagcaaaagc ctgttctggt ctgcgtcagg
     4741 tgctggaaaa cgtgatgctc aaccgcccgg aatactggca aagccactac cacggtgacg
     4801 gcaacgcgcg tcgtctggcg cgtggttata gctactcgga tcgcgtgcgc tattactggc
     4861 cggacagcca gattgatgac gctttcgctc atctggtacg taatctggcg gattcaccaa
     4921 ttccgctgcc gctgatcagc cagtatctgc cgctgcagta cgtgaaagtt cgctccggcg
     4981 agctgcagcc aacgccacgg gaactcatta tcaaccatat tcaggacatc ctggcgcagt
     5041 accacacagc ctgtgaaggc caataagcaa aacaaagagg aacacgctat gccaaatatt
     5101 gttttaagcc ggattgatga acgcttgatt cacggtcagg tcggcgttca gtgggtcgga
     5161 tttgcggggg caaatctggt gctggtcgcc aacgatgagg ttgccgaaga tccggtacaa
     5221 caaaacctga tggaaatggt actggcagaa gggatcgccg tacgtttctg gacgctgcaa
     5281 aaagttatcg acaacattca tcgcgccgcc gatcgacaga aaatcctgct ggtttgtaaa
     5341 acacccgccg atttcctgac gctggtgaaa ggtggcgttc cggtgaatcg cattaacgtt
     5401 ggcaatatgc actacgccaa tggcaagcaa caaatcgcca aaacggtttc tgtggatgcg
     5461 ggcgatatcg cagcatttaa cgacctgaaa gccgctgggg tggaatgctt cgttcagggc
     5521 atcccgacag agcctgctgt ggacctcttt aaattacttt gagggattca tcatggaaat
     5581 cagtctgttg caggcattcg cgttgggcat tatcgccttt atcgctggcc tggatatgtt
     5641 taacggctta acccatatgc accgcccagt ggtcctcggc ccgttagtcg ggctggtact
     5701 tggcgatctg cataccggta ttttaaccgg cggtacgctg gaactggtgt ggatggggct
     5761 ggccccgctg gcgggcgcac agccgcctaa tgtgattatc ggtactatcg tcggcacggc
     5821 gtttgccatt actactggcg tgaaacccga tgtcgcagtc ggtgtcgccg tacctttcgc
     5881 tgtcgcagta cagatgggga ttaccttcct gttctcggtg atgtctggcg tgatgtctcg
     5941 ctgcgaccgg atggcggaga acgccgatac tcgcggcatt gaacgcgtaa actatctggc
     6001 actgctggca ctcggcactt tctattttct ctgcgctttc ctgcctatct acttcggcgc
     6061 ggaacatgca aaaaccatca ttgatgtcct gccgcaacga ttaatcgacg gcctcggcgt
     6121 cgcgggcggc atcatgccag caatcggttt tgccgtgctg ctgaaaatca tgatgaaaaa
     6181 cgtctacatc ccctacttca tcctgggctt tgttgccgca gcctggctca agttaccggt
     6241 gctggctatt gctgccgccg cgctggcgat ggcgctgatc gatctgctgc gtaaatctcc
     6301 tgaaccgact caacctgcgg cacagaaaga ggaatttgaa gatggcatct aatcaaacca
     6361 ccctgccgaa cgtcagtgaa aacgaagaaa cattgctgac tggcgtcaat gaaaacgtgt
     6421 atgaagatca gagcattggc gcggagctga cgaaaaaaga tatcaatcgc gtcgcctggc
     6481 gttccatgct gttacaagcg tcttttaact acgaacgtat gcaggcttcc ggctggttgt
     6541 acggtctgct gcctgctctg aaaaagatcc acactaataa acgcgacctg gcgcgcgcca
     6601 tgaaggggca tatgggtttc ttcaataccc atccgtttct ggtgacattt gttatcggca
     6661 ttatccttgc gatggagcgt tctaagcagg acgttaacag tattcagagc accaaaattg
     6721 ccgtcggtgc gccgctcggc gggattggcg atgcaatgtt ctggctaacg ctactgccga
     6781 tttgtggcgg gattggagcc agtctggcat tgcaaggctc cattcttggc gcagtcgtct
     6841 ttattgtgct gttcaacgtg gtccacctgg ggctgcgttt tggtctggcg cattatgctt
     6901 accgcatggg cgtggcggcg attccactca ttaaagctaa taccaaaaaa gtcggccatg
     6961 ctgcgtctat cgttgggatg acggtaatcg gtgcgctggt ggcaacctat gttcgtttaa
     7021 gcaccacgct ggaaatcaca gcgggcgacg cagtggttaa gttacaggct gatgttatcg
     7081 acaaactgat gcctgccttc ttaccgttgg tctacaccct gaccatgttt tggttggtac
     7141 gccgcggctg gagtccgctg cgtctgattg cagttaccgt ggttctcggc atcgttggta
     7201 aattctgcca tttcctttaa atacaatgag gtattagatg ttaagtatta ttttgacagg
     7261 gcatggcggg tttgccagcg gcatggaaaa agcaatgaag caaattctcg gtgagcaatc
     7321 gcagtttatc gccatcgatt ttccggaaac ctcaagcacc gcgctgctta catcgcagct
     7381 tgaagaggcc atcgcccaac tggattgtga agacggcatt gtttttctga cggatttgct
     7441 gggcggcacg ccgtttcgcg tagcttcaac gctggcgatg caaaaaccgg gctgtgaagt
     7501 gatcaccagc accaatttgc aactgctact ggagatggtg ctggagcgcg aagggttaag
     7561 cggtgaagaa ttccgtgtac aggcgctgga gtgtggacat cgtgggctga ccagtctggt
     7621 ggacgagtta ggccgctgcc atgaagaatg tccggtcgag gaagggatat gacacacgtt
     7681 ctgcgcgcca gaaggctgct gactgaagag ggatggctcg atgaccatca gttgcgtatt
     7741 gctgacggtg tcatcgcagc aatcgaaccg attccagtga gcgtgactga acgcgatgcg
     7801 gaactgctct gccccgctta catcgacacc catgtacacg gtggtgcggg cgttgatgta
     7861 atggatgacg cgccggatgt actcgacaag ctggcaatgc acaaggcacg cgaaggtgtc
     7921 ggcagttggt tgccgactac cgtaaccgcg ccgcttagta ccattcatgc ggcgctgaaa
     7981 cgtattgctc aacgttgcca acgcggcgga cctggtgcgc aagtgctggg gagttatctc
     8041 gaaggaccgt acttcacgcc gcagaataaa ggcgcgcatc cgccggagtt gtttcgcgag
     8101 cttgaaattg ccgagctgga tcaattgatt gccgtttctc agcacacctt acgcgtggta
     8161 gcgctggcac cggaaaaaga gggggcattg caggccatcc gccatcttaa acagcaaaat
     8221 gtacgagtga tgctggggca tagcgcggcg acctggcaac aaactcgcgc cgcgtttgat
     8281 gctggtgccg acggcctggt gcattgctat aacgggatga caggtttaca tcaccgcgaa
     8341 ccgggaatgg ttggcgcggg attaacggac aagcgcgcct ggctggaact gatagccgat
     8401 ggtcatcatg tgcatccggc ggcaatgtcg ctgtgttgtt gctgtgcgaa agagagaatc
     8461 gtactgatca ccgacgcgat gcaggcagcc gggatgccgg atggtcgcta tacgttatgt
     8521 ggcgaagaag tgcagatgca cggtggcgtt gtccgtaccg cgtccggtgg gctggcgggc
     8581 agtacgctgt ctgttgatgc ggcagtgcgc aacatggtcg agttgacggg cgtaacgcct
     8641 gcggaagcca ttcatatggc gtcgctgcat ccggcgcgaa tgctgggtgt tgatggtgtt
     8701 ctgggatcgc ttaaaccggg caaacgcgcc agcgtcgttg cgctggatag cgggctgcat
     8761 gtgcaacaaa tctggattca gggtcaatta gcttcgtttt gatggtttgc tccattattg
     8821 ggccttcact tcccccgtaa ggcctttctt tttctttcgt tttgatctgt gcagcggtgt
     8881 cggatgcgac gctaacgcgt cttatccgac ctacagttgg tgaccgcaag gccggataaa
     8941 gcgtttgcgc cgcatccagc aatccctttt gcttccttta tcttttcttt caacgatcac
     9001 ggatttcgtt ttatttcctt tttctccatt gaactttcgg tttcttttct atagatttta
     9061 atcaacgaaa gacatcacca agtgaaatga aacgaaaggc aagtgaaagc gacaacgccc
     9121 gacgtcaagt tcatcagact aaggattgag ttatgccaga aaattacacc cctgctgccg
     9181 ccgcaaccgg tacatggact gaagaagaga tccgccatca gcctcgcgca tggatccgtt
     9241 cgctcaccaa catcgacgcg ctacattccg cgcttaataa cttccttgaa ccgttactgc
     9301 gcaaagagaa tctgcggatc atcctgaccg gagccggaac ctcggcattt atcggtgaca
     9361 tcatcgcgcc gtggctcgcc agccataccg gtaaaaactt cagcgccgta ccgaccaccg
     9421 atctggtcac taatccgatg gactacctga acccagccca tccgctgctg ttgatctcct
     9481 tcggtcgatc cggcaacagc ccggaaagcg tcgcagccgt ggaactggca aatcaatttg
     9541 taccggaatg ctatcacctg ccgatcacct gcaacgaagc gggcgctctt taccaaaacg
     9601 cgatcaacag cgataacgcg tttgccgtgc tgatgcccgc agaaacgcac gatcgcggct
     9661 ttgcgatgac cagcagcatt accaccatga tggccagctg cctcgcggtt ttcgcacctg
     9721 agacgatcaa cagccaaacc ttccgcgacg tggcggatcg ttgccaggcg atcctgacct
     9781 cactgggcga tttcagcgaa ggtgtgtttg gttacgcacc gtggaaacgg atcgtttatc
     9841 tcggcagcgg tggcttacag ggcgcagcac gcgagtcggc gctgaaagtg ctggaactga
     9901 cggcgggtaa actggcggcc ttttatgatt ctccaaccgg attccgtcat ggaccaaaat
     9961 cgctggtcga taacgaaaca ctggtggtgg tatttgtctc cagccaccct tacacccgtc
    10021 agtatgatct tgatctgctg gctgaacttc accgtgacaa ccaggcaatg cgtgtaatcg
    10081 ccatcgccgc ggaaagcagc gacatcgtcg ctgccggtcc acatatcatc ctgccaccgt
    10141 cacgtcactt tatcgacgtt gagcaggcat tttgcttcct gatgtacgcc cagacgtttg
    10201 cactgatgca gtcgctgcac atgggcaata cgccggatac cccatcagcc agtggcaccg
    10261 ttaaccgcgt ggtgcaaggc gtaatcattc atccgtggca ggcataagag gatcgcatta
    10321 tgagcattat ctccaccaaa tatctgttac aggacgccca ggccaatggc tacgcggtgc
    10381 ctgcttttaa cattcataac gccgagacga tccaggcgat cctcgaagtg tgcagtgaaa
    10441 tgcgatcgcc ggtgatcctc gccggaacgc cggggacctt taaacatatc gcgctggaag
    10501 agatctacgc cctgtgcagc gcctattcca caacctacaa catgccactg gcgctgcatc
    10561 tcgaccacca cgaatcgctg gatgatattc gccgtaaagt ccacgcaggt gtgcgcagtg
    10621 cgatgatcga cggcagccac ttcccgtttg ccgagaacgt aaagctggtg aaatcggttg
    10681 tcgacttctg ccactcccag gattgcagcg tggaagcaga actgggccgc ctgggcggtg
    10741 ttgaagatga catgagcgtt gacgccgaaa gtgcattcct gaccgatcca caagaagcta
    10801 aacgctttgt cgaactgact ggcgtcgaca gcttggcggt agcgattggt acggcgcacg
    10861 gcttatacag caaaacgccg aagattgatt tccagcggct ggcggaaatt cgtgaagtgg
    10921 tggatgttcc tctggtgcta catggtgcca gcgatgttcc ggatgaattt gtccgtcgca
    10981 ctattgaact tggcgtcaca aaagtgaacg ttgccacaga attaaaaata gccttcgccg
    11041 gcgcggttaa agcctggttt gcggaaaatc cgcagggtaa tgatcctcgt tattatatgc
    11101 gcgtcggaat ggatgcgatg aaagaagttg tcagaaataa aattaatgtc tgtggttcag
    11161 cgaatcgaat ttcagcataa tcattagttt tttatgattt atcccaatgt acttcccgat
    11221 taatatcggg gagtgcctta atggaaaagg agatgactaa accttaataa atagatcagt
    11281 acaatatcgc aacaataata tatttaaaaa aattatatta ttcaacttta tggtgaggat
    11341 tacacaatga gcagtccaaa tattctctta acccgtattg ataaccgtct ggttcacggt
    11401 caggttggcg tgacctggac atccaccatc ggtgcgaatc tgctggtagt cgtggatgat
    11461 gttgtcgcta acgatgatat tcaacagaaa ttaatgggta ttaccgcaga aacctacggc
    11521 tttggcattc gtttctttac tatcgaaaaa accattaacg tcatcggcaa agctgcaccg
    11581 catcagaaga tcttcctgat ttgccgtacg ccacaaacgg tacgtaaatt ggtagaaggt
    11641 ggcattgacc tgaaagatgt caacgtcggc aatatgcatt tctcggaagg gaaaaagcaa
    11701 atcagcagta aagtttatgt cgatgaccag gatctcacgg acttacgttt tattaaacaa
    11761 cgtggcgtga atgttttcat tcaggacgtc cctggcgatc aaaaagaaca aatccctgac
    11821 taaatctaaa atcgccttaa tattggtttg aggtaataaa aatgcatgaa ataaccctac
    11881 tttagggatt atccctggcg gcgttagttt tcgttctggg gattgatttt tggctggaag
    11941 ccttattttt attccgcccg ataatcgttt gtaccctaac tggcgctatt ctcggtgata
    12001 ttcagactgg cttaattacc ggtggtctga cagagttggc tttcgccgga ttaacccctg
    12061 caggtggtgt tcagccgccc aacccgatta tggcgggtct gatgaccacc gtcattgcat
    12121 ggtctacggg cgttgatgcc aaaacggcaa ttggtcttgg cctgccgttt agtttgttaa
    12181 tgcagtacgt cattctgttc ttctattctg ctttctcatt atttatgacc aaagccgata
    12241 aatgcgcgaa agaggcggat acggcagcgt tttcccgact taactggaca acgatgctca
    12301 tcgtcgcttc agcgtatgcg gtgattgctt tcctctgtac ttacctggca caaggggcga
    12361 tgcaggcgct ggtgaaagcg atgcccgctt ggctgaccca cggctttgaa gtggctggcg
    12421 gtattctgcc tgccgttggt tttggcttgc tgctgcgcgt gatgttcaaa gcacaatata
    12481 tcccttacct gatcgccggc ttcctgtttg tttgctacat ccaggtcagc aacctgttgc
    12541 cggttgccgt actgggcgca ggctttgcgg tgtatgagtt tttcaatgcg aaatcccggc
    12601 agcaagcgca accgcagccc gttgccagta aaaatgaaga agaggactac agcaatggga
    12661 tctgaaatca gtaaaaaaga tatcacccgt ctgggctttc gttcgtcgct gctgcaagcg
    12721 agctttaact acgaaaggat gcaagcgggc ggttttacct gggcgatgtt gccgatcctg
    12781 aaaaagattt ataaggatga caaaccgggc ctaagcgcag cgatgaaaga taacctcgaa
    12841 tttattaata cccacccgaa tctggtcgga ttcctgatgg ggttattaat ttcgatggaa
    12901 gaaaaaggag aaaaccgcga caccattaaa ggcctcaaag tggcactgtt tggcccaatc
    12961 gccgggattg gcgatgcgat tttctggttt actttgttgc cgattatggc gggaatttgc
    13021 tcatcatttg ccagccaggg aaacctgctg gggccgattc tatttttcgc cgtttacctg
    13081 cttatctttt tcctgcgcgt cggctggacc cacgtcggtt attcagtcgg cgtgaaggcg
    13141 atcgataaag tgcgagagaa ctcgcagatg attgctcgct cggcaaccat cctcgggatc
    13201 acggtaatcg gcgggctgat cgcttcgtat gtgcatatta acgtggtgac atcgtttgcc
    13261 atcgacagta cccacagcgt cgcgctgcaa caggatttct tcgataaagt cttcccgaac
    13321 attttaccga tggcctacac cctgctgatg tattacttcc tgcgggtgaa aaaagcgcat
    13381 ccggtgctgt taatcggcgt gacttttgtg ctctctattg tttgttccgc attcggcatt
    13441 ttgtaaatgg aacgaggcac tgcgtctggc ggtgcctctt tacttaagga atttcatcct
    13501 gtgcaaaccc ttcagcaagt tgaaaactat acggcgttaa gtgaacgtgc cagcgaatat
    13561 ttattggccg tgatccgtag caaaccggat gccgtgattt gcctggcgac cggagccacg
    13621 ccattactga cgtatcatta tctggtagaa aaaatccact agcagcaggt tgatgtcagc
    13681 cagctcacct tcgtgaagct cgacgaatgg gtggatctgc cattaacgat gcctggtacc
    13741 tgcgaaactt tcctgcaaca gcatatcgtg cagccgctgg ggctacgtga agaccagctc
    13801 atcagctttc gctccgaaga gataaatgag acagagtgcg aacgggtaac gaacctgatt
    13861 gcgcgcaaag gcggtctgga tttatgcgtt ctcggattgg ggaaaaacgg tcatcttggg
    13921 ctgaacgaac cgggagaaaa cctgcaaccg gcctgccata tcagtcaact tgatgccaga
    13981 acacagcaac atgagatgtt aaaaaccgcg ggtcgccccg tgactcgtgg gatcacctta
    14041 ggcctgaagg atattctcaa tgcccgcgag gttttgttac tggtgactgg cgaaggaaag
    14101 caggatgcga cagaacgttt tctcacggct aaagtctcta cctctatccc ggcttcgttt
    14161 ttgtggctgc acagtaactt tatttgttta attaatacct aaccagatga aaaatttgct
    14221 caaaatgttt aaattaactt atgcaacagt cacgcattat attaaataac atttgactgg
    14281 gttgaacata acgccaatag caaaaggagt tatgtt
//
LOCUS       DBFIFI010000106        14265 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.106, whole genome shotgun sequence.
ACCESSION   DBFIFI010000106 DBFIFI010000000
VERSION     DBFIFI010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14265
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.106"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(187..702)
                     /gene="pgpA"
                     /locus_tag="N7B39_003019"
     CDS             complement(187..702)
                     /gene="pgpA"
                     /locus_tag="N7B39_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HGU2422962.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGIL"
     gene            complement(680..1657)
                     /gene="thiL"
                     /locus_tag="N7B39_003020"
     CDS             complement(680..1657)
                     /gene="thiL"
                     /locus_tag="N7B39_003020"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HGU2422963.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVVPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYDHFATP"
     gene            complement(1735..2154)
                     /gene="nusB"
                     /locus_tag="N7B39_003021"
     CDS             complement(1735..2154)
                     /gene="nusB"
                     /locus_tag="N7B39_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HGU2422964.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(2174..2644)
                     /gene="ribE"
                     /locus_tag="N7B39_003022"
     CDS             complement(2174..2644)
                     /gene="ribE"
                     /locus_tag="N7B39_003022"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HGU2422965.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(2733..3836)
                     /gene="ribD"
                     /locus_tag="N7B39_003023"
     CDS             complement(2733..3836)
                     /gene="ribD"
                     /locus_tag="N7B39_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HGU2422966.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDVQTQAL
                     YPQHHLRQPVRIVIDSQNRVTPEHHIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(3840..4289)
                     /gene="nrdR"
                     /locus_tag="N7B39_003024"
     CDS             complement(3840..4289)
                     /gene="nrdR"
                     /locus_tag="N7B39_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HGU2422967.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            4440..4979
                     /locus_tag="N7B39_003025"
     CDS             4440..4979
                     /locus_tag="N7B39_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HGU2422968.1"
                     /translation="MNINVFRLILLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            5278..6162
                     /gene="tsx"
                     /locus_tag="N7B39_003026"
     CDS             5278..6162
                     /gene="tsx"
                     /locus_tag="N7B39_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HGU2422969.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(6339..6686)
                     /gene="yajD"
                     /locus_tag="N7B39_003027"
     CDS             complement(6339..6686)
                     /gene="yajD"
                     /locus_tag="N7B39_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HGU2422970.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(6774..7055)
                     /locus_tag="N7B39_003028"
     CDS             complement(6774..7055)
                     /locus_tag="N7B39_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGU2422971.1"
                     /translation="MYKLSGKAVEDFRGIYDYTLGKFGDEQADRYTDSLGTFLDTLSQ
                     MPEIGQDYDAIPEVKKIAFRFHTVYYVIRVDDILIARILHQLMEPRRHW"
     gene            complement(7048..7290)
                     /locus_tag="N7B39_003029"
     CDS             complement(7048..7290)
                     /locus_tag="N7B39_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HGU2422972.1"
                     /translation="MRKITSVSVGEQLDSFITRMVQSGRYGSASEVMRSALRLLEQQE
                     SRDEAVRNAVIEGLESGESSMTLRDIAAERKQKHRV"
     gene            complement(7442..8413)
                     /gene="secF"
                     /locus_tag="N7B39_003030"
     CDS             complement(7442..8413)
                     /gene="secF"
                     /locus_tag="N7B39_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HGU2422973.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(8424..10271)
                     /gene="secD"
                     /locus_tag="N7B39_003031"
     CDS             complement(8424..10271)
                     /gene="secD"
                     /locus_tag="N7B39_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HGU2422974.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(10299..10631)
                     /gene="yajC"
                     /locus_tag="N7B39_003032"
     CDS             complement(10299..10631)
                     /gene="yajC"
                     /locus_tag="N7B39_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HGU2422975.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(10654..11781)
                     /gene="tgt"
                     /locus_tag="N7B39_003033"
     CDS             complement(10654..11781)
                     /gene="tgt"
                     /locus_tag="N7B39_003033"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HGU2422976.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(11836..12906)
                     /gene="queA"
                     /locus_tag="N7B39_003034"
     CDS             complement(11836..12906)
                     /gene="queA"
                     /locus_tag="N7B39_003034"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HGU2422977.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            12999..13580
                     /gene="acpH"
                     /locus_tag="N7B39_003035"
     CDS             12999..13580
                     /gene="acpH"
                     /locus_tag="N7B39_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HGU2422978.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYAALETRFWQFYPRMMAQASRKAL"
     gene            complement(13601..>14265)
                     /locus_tag="N7B39_003036"
     CDS             complement(13601..>14265)
                     /locus_tag="N7B39_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2422979.1"
                     /translation="DLKTTPVDIDGVSLKSITREKIAEEIAKKTAEIIAKQNAKSIEE
                     KNIEKNKKQTNSEDNFVLKFSPEEINEMLLDYSDEEQKKLRPFFTSDHIYCLFDAFSQ
                     GLHNVARNGSPRYAGLLRQIASKSAILQDFDQQLFWRTFTGGGLHRHISVKSEPEKTD
                     VWRFSSQFDFPHINENGELSRTVLMFEGVAESNFKDIANHSSECFSYRCYAAPLDANA
                     KK"
BASE COUNT         3536 a         3831 c         3414 g         3484 t
ORIGIN      
        1 gtcgactgac gatatttcag tgtcattgaa ttatgagtga caaagataca ccttgaaata
       61 tcgccgcata gtatattcgg cgatgtcggg ttttacttta acagagtgcc ccaaaattgg
      121 ggcactgttc atttataaat caaaccgggt ggtctggaat cgcctacacc tcccggtcaa
      181 tggcatttaa agaataccca gcggccagtg atgaccgata aagtacagga tgcctgcgga
      241 aatcaccccg gcgacaatat catcgatcat gatccccatg ccgccatgta cattgcgatc
      301 aaaccagcgg atcggccacg gcttccacat atccagaata cggaaaatca caaacccggc
      361 ggcgacccac tgccagtcat tggtcggcag cgccatcagg gtgatccaca tgccaataaa
      421 ctcatcccat acgatgctac cgtgatcgtg cacccccatg tctttcgccg tttgatgaca
      481 aagatagacg ccgatacaga tccccaacat caccaccagc gagtagagct gccagggcaa
      541 aaaggtcatc agataccaga acggaatcgc cgccagcgat cccatcgtgc caggaacgat
      601 tgggcttaat ccacttccga atccgacagc aagcagatgc cacggattac tcatcttcag
      661 gcgacttttc gcgacatctt tatggcgtgg caaaatggtc atatcctttc cagtctaacg
      721 tgacaggttc gccgtcacga ataaaacaaa gcccttcgat atcggcggtc atttgcccga
      781 tacaggtaaa cggtacgccc agatgaccga gagccacatc cagcgcgcca cggttcagtt
      841 ccggcaccgt gaaacacaac tcgtaatctt caccgccaga gagcgcccag cgcagcgcct
      901 gttccggtac aacatggcga gaaagcgcat cagaaaacgg cagcaatgcc aggtcaatac
      961 gtgcgccgca gtcgctggct ttcacgatat gcccgagatc ggaaatcaga ccgtcagaga
     1021 gatcgatagc tgaatttgcc agatcgcgca gtgcctgccc ctgtaaaata cgcggcgatg
     1081 gacggagatg acgtttgatc aagtagtccg catctttagc atcggcaacc tgcaaacggt
     1141 tttgcaaaat cgccagcccg gcggcgctat cgcccggtgt accggtcaca tagatccagt
     1201 cacccggttt cgccccagag cgcgttaagg ctcgtcccat cggaacaaag ccgtggatac
     1261 ccaacgtcat tgataatggc ccacgcgtgg tatcgccgcc aatgagttgc atatcgtaat
     1321 aattgagaag atcaaacaaa ctgtcgctga aggactcaag ccacgcttcg tctacgtccg
     1381 gtaaggttaa tgccagcgtc agccaggccg gatcggcccc catcgctgcc agatcgctta
     1441 ggttcaccgc cagtgcttta taagccagat cagcaggatc gatatcaggg aggaaatggt
     1501 tgcccgccac cagcgtatca gtgctgatcg ccagggtctg tttctcgggg atattgagaa
     1561 gagcgcaatc gtcgccgatg cccagttcga catcaagacg agaacttctt acacggtcaa
     1621 aataacgggc aatcagggag aactcgccac atgccatacg ttatgcctca gcaggtaaaa
     1681 aagaaaaggc cggcgatcgc ggaacagtct tccgtgaatc taccggcctg gatattactt
     1741 tttgttaggg cgaatcacag gtgctgcttt atcgagtacg ccgttgacga acttatggct
     1801 gtcttctgcg ccgaacgatt tcgccagttc gatcgcttcg ttaatggcca ctttgtatgg
     1861 cacatcgcta cgtttagaca gttcgtacag cgcaatgcgc agtactgctt tttctacctg
     1921 tcccagttct tccagcaggc gggacaggta tggcttcatc agtccgtcga ggtatgcggt
     1981 attagtcgcc accccggcca gcagctcacg gaagtacagg acgtcaacgt cttttacatc
     2041 ctgttcagcc aggaactggt attcaacatc agcgatgtcg ttctgggaca actgccagga
     2101 gtagagcgcc tggacggcac actcacgagc gcggcgacga gcagcaggtt tcacggattt
     2161 ccccttacta atttcaggcc ttgatggctt tcaatacatt aatcatttca agcgcggtca
     2221 gtgcagcttc tgcacctttg ttgccagctt tggtgccagc acgttcgatc gcttgttcaa
     2281 tgctttcagt ggtcagaacc ccaaaagcaa ccggaatttc gctgtcctgg gcaacatgcg
     2341 ccaggccgtt gcttgcacca ccagcgacat attcaaaatg ggcagtgcca ccacgaataa
     2401 ccgtacccag cgcaatcacc gcgtcgtatt taccggtttt agccagtgca cccgccgcca
     2461 gcggcagctc ataggcacca ggcacccaaa caacggtaat gttttcatct tttacctgac
     2521 cgatacgttt cagtgcatca attgcacctt ccagcaggct gtcattgata aagttgttga
     2581 aacgcgcgat ggtgatggcg acgcgagcgt ccggggtagc aacgttagct tcaataatgt
     2641 tcattttctt ccttcgggtt cgagtatggc cccgcagggg ggcggatttt agcataatat
     2701 ttcgtgcgct gcttcccttt cgagccggga gatcatgcac ccactaaatg caggcaaaca
     2761 tccgggccta catgacgtat ctctttgaat ttaaattggg gggcgtcagc taatttctca
     2821 agccctggca gtgtgcataa tccacgggcg tcgctgccta atagtttagg tgcgatatag
     2881 acaatcagct catcgactaa ccccgcctgc agcaatgcgc cagcgagcgt tggccccgct
     2941 tccacccaga tgctgttaat ctgctgttta cccagttgca tcatcagtac aaccagatcc
     3001 agatgacctt tatgctctgg aatcagcaag gtacgcaccg tttccggcca ctcacgagaa
     3061 tcttcctgcg tacgcgcgaa ccaggtttcg ccgggctgct gcacaatgtg atgttccggc
     3121 gtcacgcgat tttggctatc aatcacaata cgtaccggct ggcggagatg atgttgcggg
     3181 tagagcgcct gagtttgtac atccagttca gaccaacgca ccgttaaggc aggatcatcc
     3241 gccagcaccg tagcgctgct ggttaaaatg gcatgacttt gtgcgcgcag tcgttgtacg
     3301 tcgcgccgcg cctgaggcga agtgatccac tggctttcgc cgctcgccat cgccgtgcgg
     3361 ccatcaagcg atgcgccaag ttttaactga atataaggaa agccggtgcg catccgcttg
     3421 agaaagcctt tattcaattg ctcggcttca ctcatcatca ggccgtggct gacgtcaatg
     3481 ccagcctgtt gcagacggta aagtccacgc ccagcgacct gcgggttcgg gtcctgcatc
     3541 gcagcgacca cgcgcgctac gccagcggcg atgagtgcgt cacagcacgg tggcgtacga
     3601 ccatgatggc tacagggttc gagtgtgaca taggcggtcg cacctttggc tttttcaccc
     3661 gccatacgca acgcgtgtac ttcggcatgt ggttcacccg ctcgttggtg gtaaccttca
     3721 ccgacaattt cgccatcttt gacaatgacg cacccgacat tcgggttggg gtgggtggta
     3781 aaacgtcctc gttgcgccag ctttagcgcc cgcgccatgt aatactcgtc ctgcacggct
     3841 tagtcctcca ggcgcgcgat ctcttcgcca aattctttga tatcttcgaa actgcggtag
     3901 acagaggcaa aacggatata ggcgacttta tcgagctttt tcaattgctc catcaccaga
     3961 ttgccaatca tcttgctcgg cacttcgcgc tcaccggtgg cgcgcagctg cgatttaata
     4021 tgattgattg ccatttcgac gtcatcggaa ctcaccggac gtttttccag cgcccgcagc
     4081 attccgctac gtaatttttc ttcattaaac ggttcacgca cgtcgttgct ttttacaaca
     4141 cgcggcataa ccagctccgc cacttcaaag gtggtgaaac gttcattaca caccagacac
     4201 tgccgacggc ggcgtacgga tgaaccctcg cccacgagac gagagtcaat taccttagtg
     4261 tccacggcga aacagaatgg gcaatgcata cggcgtcctg accaggtggt taacggaaat
     4321 ctattttacc ctgaactgac gatacaacaa aggcgcaacg cttttgagat aaaggatcga
     4381 tgctttcgct gcttttaccc tctaccatta ggctaattgc gacgagaaag ggacgaccta
     4441 tgaatattaa cgtttttcga ctgatcctgc tgggaagcct attcagcctt agcgcctgtg
     4501 tgcagcaaag tgaagtgcga cagatgaaac acagcgtcag cacgctgaac caggagatga
     4561 cgcaactcaa tcaagaaaca gtcaaaatca ctcagcaaaa caggctgaat gcaaaatcca
     4621 gcagtggggt ttaccttctg cctggagcga aaacaccggc aagactggaa agccagatcg
     4681 gtactttacg tatgtcgctg gtgaatatta cgcctgatac agatggcacc accctgacac
     4741 tacgtattca gggtgagtcg aatgatccct tgcccgcatt cagcggaact gttgaatatg
     4801 ggcaaattca gggaacaata gacaactttc aggaaatcaa tgtacagaat caattgatta
     4861 atgcccctgc cagcgtcctc gcccccagcg atgttgatat tccgttacag ttaaagggga
     4921 tttctgtgga tcagttaggc tttgtacgta tccacgacat tcaacctgtt atgcagtaaa
     4981 cttatagcgg ggcgattttg tgcgtcccgc aacatccttc cccatcattt tgttactccg
     5041 cttacataac ccagattgat agtaaaagtt tgcaacaagg gcgaaagtca gtacaatccc
     5101 cgcccgaatg tgtgtaaacg tgaacgcaat cgattacgta aatgatagaa ctgtgaaacg
     5161 aaacatattt ttgtgagcaa tgatttttat aataggctcc tctgtatacg aaatatttag
     5221 aaacgcaatt tgcgcctttt tcactcccgc aagggatttt caaacagtgg catacatatg
     5281 aaaaaaacat tactggcagc cggtgcggta ctggcgctct cttcgtcttt tactgtcaac
     5341 gcagctgaaa acgacaaacc gcagtatctt tccgactggt ggcaccagag cgttaacgtt
     5401 gtcggaagct atcacacccg tttcggaccg cagatccgca acgataccta ccttgagtac
     5461 gaagcattcg ctaaaaaaga ctggttcgac ttctatggtt atgcggatgc gccggtattc
     5521 ttcggcggta actccgatgc aaaaggtatc tggaaccacg gttctcctct gtttatggaa
     5581 atcgaaccac gtttctccat cgacaagctg accaatactg accttagctt cggtccgttc
     5641 aaagagtggt acttcgcgaa caactacatt tacgacatgg gtcgtaataa agatggtcgc
     5701 cagagcacct ggtacatggg tctgggtacc gatatcgaca ctggcctgcc gatgagcctg
     5761 tccatgaacg tctatgcgaa ataccagtgg cagaactatg gcgcagcgaa cgaaaacgag
     5821 tgggacggtt accgtttcaa aattaaatac tttgtgccga ttaccgatct gtggggcggt
     5881 cagctgagct acatcggctt caccaacttc gactggggtt ccgatttagg ggatgacagc
     5941 ggtaacgcaa tcaacggtat taagacccgt actaataact ctatcgcttc cagccatatt
     6001 ctggctctga actacgatca ctggcactac tctgtcgtag ctcgttactg gcacgacggt
     6061 ggtcagtgga acgacgatgc agaactgaac ttcggcaacg gcaacttcaa cgttcgctct
     6121 accggctggg gtggttacct ggtagtaggt tacaacttct gattatgaaa atgccgggat
     6181 ttattcccgg catttcagat tgttaacaac gtacgctttg ttcatgccgg atgcggcgta
     6241 aacgctttgt ccggcccaca aaagcacgta aattcaatat attgctgaaa taacgtaggc
     6301 ctgataagcg tagcgcatca ggcaatttta cgttttaatc acttcttctt gttcatcatc
     6361 gctttcaggt cagcgaatgg gttgtacttc gcttcaccga catctttctg cgcatcttcc
     6421 cctgcgataa cggtcgtacc atactgatcc gcttcggtat atttcgaatg ctcatgatcg
     6481 tggcaataga gacaaagcaa ttcccagtta ctgccatctt ccgggttatt ggtatggtcg
     6541 tgatcaatgt ggtgaacggt aagttcacgc aggttggaat aaacaaactc gcgggaacag
     6601 cgaccgcaga cccacggata gatttttaat gctttttcgc gatagccgct ttctaaccgc
     6661 gcatagtttt ttgggatgat agccatgaaa agttacctgg atgaaatgta gggttacgcg
     6721 cagtttccca agatgcgggg attttcgcaa tcctgagtca agaaattctc acattaccag
     6781 tgacggcgag gttccatcaa ctgatgaaga atgcgcgcaa tgagaatgtc atcgactctt
     6841 ataacgtaat aaacagtgtg aaacctaaat gcgatcttct ttacctctgg aatcgcatca
     6901 tagtcttgcc ctatctcggg catttgagag agtgtatcca gaaaagtgcc gagagagtcg
     6961 gtatagcgat ccgcctgctc atcaccaaac tttcccagcg tgtagtcata gatccctctg
     7021 aaatcttcaa cagcttttcc gctaagctta tacacgatgt ttttgcttcc tttctgctgc
     7081 gatatcacga agcgtcatcg aactttcacc gctctcaaga ccttcaataa cagcatttct
     7141 gacagcttca tccctggatt cttgttgttc caatagacga agtgccgaac gcattacttc
     7201 actggccgag ccataacgac cagactggac catccgggta ataaaactat ctaattgttc
     7261 gccaacgctt actgaggtaa ttttgcgcat aagcgatctc cacgtattaa ctattaatac
     7321 aaagtatatg tcagaacgct ttctacaacc aaaccgtatt cagttgcctg tgaaacaggc
     7381 aaaaaaaaac cgcagcgcaa atacgccacg gtttttttaa cagaacggat ttaaaacacc
     7441 attacggcag aattgacggc tgatccgccc cttctttttc cactttctgc tgcaacatgt
     7501 gttcgcgctt catacccagt ttcagagcca acgcagatgc cacatagatg gaagatgcag
     7561 taccgatgga aacaccgata agcatggtca gcgagaagcc ttccagtacc ggaccaccga
     7621 agagatacag catcaggata accatcaagg tagtaccgga tgtgatcaag gtacggtgca
     7681 gcgtctgggt caaggacacg ttaaagattt cgtaaggcgt accgcgacgg atcttgcgga
     7741 agttttcacg aattcggtcc gataccacga tactgtcgtt aagcgagtaa ccgataaccg
     7801 acatcaacga tgccacaatg gtcaggtcaa tctcgatatg gaataacgac aaaataccca
     7861 gcgtaatgat aacgtcgtgc gccagcgcaa taaccacccc tgccgccagt cgccactcaa
     7921 agcggaaacc tacgtacacg aggatagaca gcagcgctgc catcaacgcc atcgcaccgg
     7981 tttgcgcaag gtctgccccc acgctcggac cgacgaactc aatacgcttc actgctgcat
     8041 tctgattggt ggattcgtta atcaccttca gaacctggct gcccagcacc tgaccgccgg
     8101 tttcgccttc agcaggcggc atacggacca tgatgtcgtg gctgctacca aagttttgca
     8161 gcatcggctc ttcaaaaccg gctttttgca atgcatcacg cattacgtca atttcagccg
     8221 gtttttcgag cgtaatttcg ataaccgtac caccggtgaa atccagcccc cagttaaagc
     8281 cgcgcacgcc cataataacg atagcagcga ttaacagcag accagagatg ccaaaagccc
     8341 agtagtccca gcgcataaag tcatagactt tacggccgtg gtttagttgt tcaacagtat
     8401 attcctgtgc cacatcgcac tcctcagatt gacagcttct tgacgcgctt gccgccatat
     8461 agcaggttta cgatggcacg cgtaccgaca atcgcggtaa acatcgacgt cgccacaccg
     8521 ataccggtag taatcgcgaa ccctttaatt gccccggtac ccactgcgta caggatgatg
     8581 actttaatca gcgtggtgat gttcgcatcg aagatagaac tgaatgcgcc acgataacct
     8641 tcatcaattg cctgttgaac agtacgtccg ttgctcaact cttctttaat acgttcgttg
     8701 atcagtacgt tcgcatcgac cgctaccgca agggttaaga cgatacccgc gatacctggc
     8761 atactcagtg ttgcgcctgg caacagcgac ataataccga cgatcaagat caagttggca
     8821 atcagagcac tggtcgcaat cagaccaaac ttcttataga agatgatcat gaacagaata
     8881 gaaaccagca gaccggcaag gcaagcctcc agcccctgct caatgttctg catacccagg
     8941 gtcgggccaa ttgtacgttc ttcaacaatc tgaatcggcg cgatcaacgc accggcacgc
     9001 agcagcagtg acagctgacg ggcttcgttc gggttgttga tgccggtgat acggaagctg
     9061 ttacccagac gagactggat gttggcgatg ttaatcacct cttcctgttt caccagaacc
     9121 gcacgaccat ttgcatcttt cttaccgctg tctttgtact ccacaaacag ggttgccatc
     9181 ggtttgccga tgttgtcctt ggtgaagtta gacataatgt tgccaccagc gctatcgagc
     9241 gagatgttaa cctgcggctg gttgtattcg tcctggctgg aagtggagtc ggtgatatgg
     9301 tcaccggtaa gaattacgcg tttgtacagc acaaccggct gaccttcgcg ggtctgtttc
     9361 acttcagagt cgcccggtac gcgaccggat gccgcagcgg cctgatcaac gttggtgttt
     9421 accagacgga attccagcgt tgccgtcgca cccagaatct ctttcgcacg cgcagtgtcc
     9481 tgaatacctg gcagttcaac aacgatacgg tcagcaccct gacgctgaac caccggctcc
     9541 gccacgccaa gttggtttac acggttacgc aggatattga tgttctgctg caccgcatat
     9601 tcacgcgctt cactcagacg agcatcgctc attaccgcac gcagctggtt gctgccctga
     9661 ctgctaatca ccaggtccgg atgacgcttg ctcagatacg caatggcttc atcacgagct
     9721 ttagcatcgc ggaaagtgat gctcaggccg tagttatttt ctttacgaac ggtggtatac
     9781 gggatgcctt tttcgcgcag gtcgctgcgc aggctatcga tattttgttc ctggagtttg
     9841 ccaagcgcgg tgtccatatc cacttccatc aggaagtgaa cgccgccacg caggtcaagg
     9901 ccgagcttca tcggctcagc gtgaatagct gccagccagc gcggcgttgc cggggcaagg
     9961 ttaagcgcca cgacgtattt gtcacccata acgcccatta atgcttcacg agcgcgcagc
    10021 tgggtgtcag tggagtcgaa gcgcgcaaga atagcgccct cttccagtgc cacagactta
    10081 gcagttattt tttcttcttg taacgttttc tggacctgga tcagcgtttg ctcactggcg
    10141 gcgactccgc gcgcaccagt gatctgaaca gccggatcct caccaaacag gttgggaagc
    10201 gcatacagca gaccgatgac aatcaccacg atcagcatga cgtacttcca caaaggataa
    10261 cggtttaaca cggcaattcc cttagggaaa aattttaatt acagcgcctt catggtgcct
    10321 ttcggcagga cggcagctac gaagtcacgt ttaataacta cttcagtggt gtcattcagc
    10381 gcgatagcaa tgtagccgtt ttccgctact ttggttacgc gaccaaccag gccaccgttc
    10441 gtcagaactt catcaccttt ggcaatggag tccatcagct ttttgtgttc tttggtgcgc
    10501 ttctgctgtg gacgcaggat catgaaatag aaaatcagac cgaataccac cagcatcaaa
    10561 atcaaagaca tcgggctacc ttgcgccggt gcacccgttg ccgctaccgc atcagaaata
    10621 aaaaagctca ttaaatttcc ctcattatta atattaatca acgttcaaag gtggtacttc
    10681 tcgcccctga cgctggtaaa aatcagttac gaagctctct aatttaccct cttcaatagc
    10741 cttgcgtaaa cccgccatca aacgctggta gtaacgaagg ttatgaatgg tattgagtcg
    10801 cgcgcctaat atttcgttgc aacggtcaag atgatgcaag taagcgcgtg aataattgcg
    10861 acaggtgtag caatcacact caggatcgag tgggccagta tcgctcttat acttcgcatt
    10921 gcggattttc accacgccat cggtcacgaa caaatgacca tttcgggcgt tgcgggttgg
    10981 cattacgcag tcaaacatat cgataccacg acgtacgcct tcaaccaggt cttctggttt
    11041 accaacgccc atcaggtaac gcggtttgtc tgccggaatt tgcgggcata catgctccag
    11101 aatgcggtgc atatctgctt tcggctcacc cacagccaga ccgccgacag cgtagccatc
    11161 aaaaccgata tctaccagac ctttaacaga aatatcacgt aaatcttcgt aaacgctgcc
    11221 ctgaatgata ccaaacagcg catttttgtt tccgagactg tcaaaacgct cacggctacg
    11281 cttcgcccaa cgcagagaca tctccatgga gcgttttgcg taatcccagt cagcaggata
    11341 cggcgtacac tcatcaaaga tcatgacgat atccgaacca agatcgtact gaatctccat
    11401 cgacttttcc gggtcgagga aaatcgggtc gccgttgatc gggttacgga agtgcacgcc
    11461 ctgttcggtg attttacgaa tatcgccaag gctgaagact tggaagccgc cggagtcggt
    11521 gaggatcggt cccttccact gcataaaatc gtgcagatcg ccgtgcagtt tcatgatttc
    11581 ctggcccggg cgcagccaca ggtggaaggt gttgccgagg ataatttgcg cgccagtggc
    11641 ttcaacttct tccggcgtca tcccttttac ggtgccgtag gtgccaacag gcataaaaca
    11701 aggcgtttcc actacgccac gatcaaagac caggcggcca cggcgtgcgc gaccgtcggt
    11761 ggtgtccagt tcaaatttca ttttttctcc actacgtcag aaaaacagtc caacgtttaa
    11821 accagcgccg cggaattact ccccgacgcg ctcattaatt gcctgcggat tgtacgtgat
    11881 aaacatcgca tcaccgtaac taaaaaagcg atatttctct tctaccgccg ctttataggc
    11941 attcattgtg tgttgataac cggcaaaggc cgaaaccagc ataatcagcg tcgattccgg
    12001 aaggtggaag tttgtcacca gcgcatcgac cactttgtac tggaagcccg gatagataaa
    12061 gatttgggta tcgtcaaaga acggttcgat gagatcgttt ttcgccgcct gagccgcgct
    12121 ttccagcgaa cgtactgagg tggtgccaac cgcaatcacc cggttaccgc gcgctttcgc
    12181 cgccagtacc gcgtctacca catcctgcgg tacttcagcg tattccgagt gcatgatgtg
    12241 atcttcaatg gtgtcgacgc gtaccggctg gaaggtgccc gcaccaacgt gcagggtgac
    12301 gaacgccatc tccacgcctt tggcgcgtaa tttttccagc aaaggttcgt caaaatgcag
    12361 accagcggtc ggggctgcaa ccgcgcccgg tttttcgcta taaacggttt gataaagttc
    12421 gcggtcagcg tcttcgtccg gacggtcgat atacggcggc agcggcatat ggccgatgct
    12481 gttgagaata tccagcaccg agcgttcatc attaaattcg acttcaaaca gtgcgccgtg
    12541 gcgcgcggtc attgttgcgt taatactttc gtcatcgccc agcagcagtt ctgcgccagg
    12601 ttttggcgct ttcgaggcgc gaatatgcgc aagaatgcgt ttgtcgtcga gcatccgttc
    12661 aaccagcact tcaatcttgc cgccgctggc tttacgccca aacaggcgcg ccgggatcac
    12721 ccgggtatta ttaaaaacca gaagatcgcc ggggttgagc ttatcgagta aatcggtgaa
    12781 agtaccgtgc gtcagcgcgc ccgtcggccc gtccagcgac agtaaacgac agctactgcg
    12841 ttcaggcatc ggatagtggg caatcaggga ttcgggcaat tcaaaggaga aatcggtaac
    12901 gcgcatgaca ctgactcaga ctaaaataag aggcgggtag tctagtgccg gggcgctctc
    12961 cctgcaacct ttacccccct ttggatagaa tataatcgat gaatttttta gctcacctgc
    13021 atttagccca tctcgcggaa agctcgcttt ccggcaattt gctggctgat ttcgtacgcg
    13081 gaaatcctga agaaagtttt ccgcccgacg tcgtggctgg cattcatatg catcgacgta
    13141 tcgacgtatt gactgacaat ctgccggaag tccgcgaagc acgggagtgg tttcgtagtg
    13201 aaactcgccg cgttgcgcct attacgctgg atgtcatgtg ggatcacttt ctttcccgtc
    13261 actggtcgca gctgtcgccg gactttccgc tacaggaatt tgtttgttat gcccgcgagc
    13321 aagtgatgac aattttgccg gactcaccgc cacgctttat caacctgaac aactacttat
    13381 ggtcagagca gtggctggtg cgctatcgcg atatggattt catccagaac gtgttaaacg
    13441 gcatggcaag ccgtcgccca cgtctggatg ccctgcgtga ctcctggtac gatttagacg
    13501 cccattatgc cgccctcgaa acccgcttct ggcagtttta tccacggatg atggcgcagg
    13561 cgtcacgcaa ggcgctctga aacttcaaac tccggcgtca ctatttttta gcattcgcat
    13621 ccagcggggc ggcataacaa cggtaagaaa aacattcgct ggaatgattg gcgatatctt
    13681 taaagttgct ttcagcaacc ccctcgaaca tgagtaccgt tctggacaac tctccgtttt
    13741 catttatatg tggaaaatca aattggctac tgaatcgcca tacatctgtc ttttctggct
    13801 cgctcttcac agagatatgt cgatggaggc cccctcctgt aaacgtgcgc cagaacaatt
    13861 gttgatcaaa atcttgtaat atcgcagact tggatgctat ttgacgtagt aaacctgcat
    13921 accgtggact gccattacga gcaacattat gcaaaccctg actaaaagca tcaaacaaac
    13981 aatagatgtg atcactagtg aaaaaaggtc ttaatttttt ttgttcttca tcgctgtaat
    14041 ccaggagcat ctcattgatt tcttccggtg agaattttaa gacaaaatta tcctcagaat
    14101 ttgtttgttt cttgtttttt tcaatattct tctcctcaat gctcttcgca ttttgtttcg
    14161 caattatttc cgcagttttt ttcgcaattt cttccgcaat tttttcccga gttattgatt
    14221 ttagactaac accatcgatg tcaaccggtg ttgttttcag atctc
//
LOCUS       DBFIFI010000107        14036 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.107, whole genome shotgun sequence.
ACCESSION   DBFIFI010000107 DBFIFI010000000
VERSION     DBFIFI010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14036
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.107"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..436
                     /locus_tag="N7B39_003037"
     CDS             <1..436
                     /locus_tag="N7B39_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2422980.1"
                     /translation="MNHRAHIDRRLAPIANMLLTYSESLPTGCDVLPTVSREQFNEAK
                     QMLLRNPDSFGRISIFPKELWSSAITPELKSVFNKLWIEDKYHSRLEKRFNLMLVAAL
                     LTQKDNNLIHQLSALFEFHYQQAPQQLGVIYQYYFEQAGVCH"
     gene            436..6750
                     /locus_tag="N7B39_003038"
     CDS             436..6750
                     /locus_tag="N7B39_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGU2422981.1"
                     /translation="MDVQQLHYAPLLNQLHQRAVRSVVSQLALRSKSLTHYLESQYFQ
                     PPGQEGSLLADPVFESTFGWMPADETMEDLRDKLISSPLVDALSKAPVPFRHQLTAWR
                     TILEDKKSLLVSSGTGSGKTECFMVPVLEDLIRQQKSTRQSLTGTQAIFLYPLNALIN
                     SQQERLREWTRGFKGKIRFALYNGETRHTKYEIQEDQLKVPEQALSREAIYEEPPSIM
                     VTNTTMLEYMLIRRKDAPIIEKSQGMLKYIVLDEAHSYIGSQAAELALLLRRVMQAFN
                     VGPGTDKPVQIIATSATIGEDSPEGNKELAKFVADLAGVTERDVKVVRGYRQIPRVSE
                     SLIRHEYPTSLTSLKSLSPQDLYQQLCHYRVAQQLRQALTHPGRQAVRLSELLNVARR
                     TWPDINHRELLQLLDLMARSREGELAFTPLRMHGFIRTLAGLWACSNKQCSHKAHELN
                     QSDWPFGQVWFEQRQYCDCGAPVFEVLRCSGCGSAYLSAKEEMRGDGTNWLVAQPATA
                     EVDEFALDVDVYSEDEDNDELDNNSQFDRLIASDGEKYIISLEETTAGKIDSEAQKHY
                     EINLLRPESRGEGRNNSFACVCCGDTQRKNNPLFRPLRLGAPFFLNEIIPTLLEFSPL
                     PQTREEQLGPFNGRRLLTFTDSRQGTARISARLQQDADKATLRALCYQELANITEPSA
                     KLSPAHCALLEKVISQFSVLPLKNSIDALKKAMAANEELSPTDIQALKIIEPMVSSLS
                     TEHAEAMQILQANSGNQQKKTMSWQTLSEQLVGSVDLADRMRGSFKELSGLDLTKQQF
                     ADFCLYSEFGRRPKNAWTLESLGLVAIHYPFIDKVTTCPQDWKTLLPDPDKQLAEWRN
                     LLKITLDFFIRENSAVFYENEQYPRWMGARFPVKMLQGPDKKNKGQKRDQLWPQIRDR
                     HYNRVIKLLIAVFPAIQPEQAHWKSLVNHLMIEVWDAIRPCLRQFESGYQLDIKQQAE
                     FYSPKQVWRCPYTRRALDVTLLGYSPYLPGSKEIAPEKAVLIEMPELPVRHWRLSGGG
                     EIAREERLEWLESNALIQHAREEGLWSTRSDRLALKDSWYRLEEHSAQRTPEQNQFNE
                     KQFKSGKVNVLNCSTTMEMGVDIGGMSLVAMNNVPPAPANYLQRAGRAGRRGESASAA
                     ITLCKNTAHGMEVFKDPLWAFNTTASAPRVRFGSSSIVQRHVNALVLGLFLRAEVPDA
                     TKLSCKWFFEGDESQCLRFLHWLNHQADQLADKLKRLTQGTVLMSLTATQLLTRTQAM
                     MQQVDIRWRSQLAILLENIEALKADNSAWEETPAGKAIAYQLRDYRGAYLFSKLISEA
                     FLPGHGFPVGVVNFNYLTADELEKRRAIKATQADPNEGGESFSRRIEKLPSRDLPTAL
                     REYAPGADVVLGGKVYRSSGIMLGKVLASGQELSGDHHIPWFWHCRKCGAGATSTTHP
                     VECSHCKADIQQLDVKRYLQPVGFATDIRYQAHNDVSMPAQLPWKDPRVLVPSSVWVS
                     LPDAGLGRYRFSHSGELFHFSEGEFGHGYAICLSCGRAESQTQPQRTPENLKNPEREN
                     THYLLRGGSNDRQGSNKLCHGHVHKDLWLGYSSRTDMVELQLNDDNGLLIRDEVAARS
                     LAVALREGLAHKLGIENTELGVTTQQARDINGYTGYSIFIYDNNAGGAGYAVQLIDHW
                     ADVFNYARKLLDCSCDKFCHHCLLSYDSQHYVNRLDRHHALTLLTNVRLQRLNLAPEY
                     QYFGDGSRVETNPLSLRIAQCLNSEIYDSCSLVLAGPQEQWDFAQWPLFKELLQFASS
                     GGNVELLVATPLANLTDSSRHQLSALAAMPGGRLQVKSIATAQLMQGKGRWLAQVTRE
                     GQSQQWAADDSATVAPGELWGQSASSPVVTLKGTSGKTFSGQTLSAEDLLPALPTGAV
                     RINLCEQLDGPLEGFGSRFWSLVTQQHAGWKQAFTRHKEITHVEYSDRYLNSPFTARL
                     LGEILTELVEQGMAERASLTVCVKKLDYNSRQHDALYNAWLNEEDRQQVVTTLLEEGY
                     LGPAWPGAISWLTGDNQSTEHGRELTVTFSDGSQHYVLLDMGLSYWRCIEDTFFDFAL
                     RVPQQVERLANTRARAVAPGNDLRSYIIAG"
     gene            6974..9832
                     /locus_tag="N7B39_003039"
     CDS             6974..9832
                     /locus_tag="N7B39_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGU2422982.1"
                     /translation="MNKQNYAPGMRVVIRDAEWRIRRADDSGDGGYLLTCDGISELVR
                     GKEGLFLTKLEQKVEILDPAKTHLVEDESANYQAAQLYIESQLRQRVPTDSKVHFGHL
                     AAMDSMPFQLDPTRMALAQPRQRILIADAVGLGKTLEAGILVSELIRRGRGKRILVLA
                     VKSMLTQFQKEFWSRFAIPLTRLDSAGLQKVRNRIPTNHNPFHYFDKTIISIDTLKQD
                     IEYRHHLENAWWDIIVIDEAHNVAERGTSSLRSKLAKLLAGRSDTLIMLSATPHDGKA
                     ESFASLMNMLDPTAIANPKEYEYADFADKNLVVRRFKKDVKDQMSGEFPERNIVKLTR
                     LASGAEEEAYRRLVESQFRDDDDEQAQSNKGRLFKITLEKALFSSPMACASVVANRLK
                     RLESRKDHNSQSQINELESLLLALNNIDASQFSKYQLLLDTIRKDLAWKANNTEDRLV
                     IFTESIKTLEFLEQQLRADLKLKDDQIATLRGDQGDTVLMETVEAFGKTQSPLRLLVC
                     SDVASEGINLHHLSHKMIHFDIPWSLMVFQQRNGRIDRYGQKHQPQIRYLLTEASEPQ
                     INGDMRVLEVLINKDEQAQKNIGDSSEFTGKFTQEEEEEQVAEFMMQDDGASLFDQLL
                     NSNVSESAEHDLFGEICSAVSSDASMVTETDTSLFASEQAYCERALGYLKASGQTIQY
                     ETLPDNTLSLVAPEELRRRFNQLPPEIAPENWQLYLSQDKTVITDAIARARGEQHAWP
                     DVQYLWQINPVVQWLDDKISSAFGRHQAPVIRLPYLLEPDEDHFILSGLFPNRKSHPM
                     VNPWIVVSFNRESLIGSQPFAEFLQRHPQLSNKLTNSGGKDRNHQRQQDLLEAAIAHA
                     REVFIHDRNAFETHINQQLNEHLQKLDVLRGRQLSQLELDFADNKQQLSVKQSRKEQR
                     QREIEHNFDSYIEWIEDTMTTEKEPYIQVIAVITGAEG"
     gene            9835..>14036
                     /locus_tag="N7B39_003040"
     CDS             9835..>14036
                     /locus_tag="N7B39_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2422983.1"
                     /translation="MALVGINNENEFYSNHYLGEVFTSDIRDVLEPWIAQENAAREAE
                     RAAREQGKDVEPGYRAPWNQFNSLATEFFRKLAEHEKQRQIPQRLADQRNRWQPLLKA
                     LGYEITPQIQMLDDDTPLPVLARYNSTDGSPWLWIVEAHDQEEGTLDPLALSLLTAQF
                     PADTDKHKHDSLRKKANGEYRSWQDLLSTAVFTQNEPPRFVLLLGNRQLLLLDRTKWA
                     QNRLLRFDFEEILSRRETDTLKATAVLLHKDSLLPGSGAPYLDSLDDNSHKHAFGVSE
                     DLKYALRESIELLGNEAMHYLIDRGLANYTGNRAVDPDELSRECLRYMYRLLFLFYIE
                     ARPELGYAPMTAKTYLQGYSLETLRDLEMIPLTSEEDRNGRYFHDSLNMLFKLVREGY
                     NGGVKMQSDLESGDRITIHSHQFSVPRLESHLFDANNTRILNRVVFRNETLQQIIQAM
                     SLSRPAKGRFNRRGRISYRQLGINQLGAVYEALLSYRGFFASEDLYEVKKAGEEFNEL
                     ETGYFVSKDEISKYHEDEKVYEKDGSLRIHRKGSFIYRMAGRDREKSASYYTPEVLTR
                     SLVKYALKELFKEQIDPISDPHAKADAILNLTVCEPAMGSAAFLNEAINQLAEAYLFH
                     KQQAEGRRIPQDRYTQELQRVKMYIADNNVFGVDLNPVAVELAEVSLWLNAISGDAFV
                     PWFGYQLHCGNSLVGARRQVFNKSELTYKKAKDPSWLNSEPVELAMNTPREETQIFHF
                     LLPDGGMANYSDKTVKQRYPDDFKALDSWRKEFIKSFAGHEIADVQRISEKVEALWNT
                     YRQQLKAERLKTADSYPVWPAENSEQTRSSLSSKDETFSGRLEDNSAYQKLRWVMDYW
                     CALWFWPIDKADELPDRGTWLFEIETLLDGIVITEKVTEVAEHTTGDLFAEEGLLREE
                     SSLFSVAGRLKTEVLFRHLPRLAIVDALRKQHRFFHWDLEFCDLFAERGGFDLMLGNP
                     PWLKVEWQEAGVLGDYEPEFVLRKLSASKLATLRIDTFNQIPALEAAWRSEYEGCEGM
                     QNFLNAQQNYPVLRGVQTNLYKCFLPQAWRLGAQKGVAGFLHPEGIYDDPKGGQLRAA
                     VYPRLRAHFQFQNELNLFVEVDHHAKFSSNIYSASPSTVGFEHISNLYAPQTIDACFE
                     HSGSGDIPGLKDEIESEGKLKVVWNTSGHRSRLISIATHELELFARLYDSEGTPAWQA
                     RLPALHAKQLVAVLEKFANQPNRLGDLQGQYFSTVMFDETYAQRDGTILRQTQFPQDS
                     SQWVLSGPHFFVGTPFYKTPRENCTLNSDYDCLDLLTLPDDYLPRTNYIPACDAQEYA
                     KRTPCVTWTELAEDEPKKVTDYYRLAIRAMLAQSGERTLISAIYPPEISHMNAVRSYC
                     YSSQNLLLEHSGMCFSLPFDFI"
BASE COUNT         3622 a         3279 c         3701 g         3434 t
ORIGIN      
        1 tatgaaccat cgtgcacata tcgatcgtcg gctggctccg attgcgaata tgttacttac
       61 atatagtgag agcctaccaa ccggttgtga tgtattgcca actgttagtc gtgagcagtt
      121 taatgaagct aaacagatgc tattaaggaa ccccgacagc tttgggcgta tcagtatctt
      181 ccctaaagaa ctttggtcta gtgctattac tccagagtta aaatctgttt ttaataagct
      241 ttggattgaa gataaatatc actcacggct tgaaaaacgt tttaatttga tgttagtcgc
      301 agcgctgtta acccaaaaag ataataacct gatacatcaa ctgtctgcgc tttttgaatt
      361 tcactatcag caagccccac agcaattagg ggtaatctat caatattatt ttgaacaagc
      421 aggagtatgt cattgatgga cgttcagcaa ctgcattatg cgccacttct aaatcagctt
      481 catcagcgtg cggtgaggtc ggtggtaagt cagttggcat tgcggagcaa gtcgttgact
      541 cattatctgg aatcacaata ttttcagcct cccggtcagg aggggtcact gcttgcagac
      601 ccggtattcg aaagtacatt cggctggatg cctgcggatg aaacaatgga ggatttgcgg
      661 gacaagctga tctccagccc gctggttgat gcattgagca aagcgccagt gccatttcgt
      721 catcaactta ctgcgtggcg aacgattctg gaagacaaaa aatccttgtt agtgagcagc
      781 ggtacgggat ccgggaaaac agaatgcttt atggtaccgg ttctggaaga tcttattcgt
      841 cagcaaaaat cgacccgcca gtcactaaca gggacacaag ctattttctt gtatccgtta
      901 aatgcattaa ttaacagcca acaggagcgg cttcgcgaat ggacgcgcgg atttaagggg
      961 aaaatacgct tcgcgctata taatggcgaa acccggcata ccaaatacga aattcaggaa
     1021 gatcagttga aagtacctga acaagctttg agtcgggaag ccatttatga agaaccacct
     1081 tccatcatgg tgacgaatac caccatgctg gaatatatgc tgattcgtcg taaagatgcc
     1141 ccaatcatcg aaaagtctca ggggatgtta aagtatatcg tcctggatga agcccatagt
     1201 tatattggtt ctcaggctgc tgagttagcg ctgcttttac gtcgtgttat gcaggctttc
     1261 aatgttggac ccggcacaga taaacctgtg cagattatcg cgacctctgc cactattggt
     1321 gaagactctc ctgaaggtaa taaggagctt gctaaatttg tcgctgatct cgcgggcgtt
     1381 acagaacgtg atgtcaaagt ggttcgcggt tatcgacaaa taccgcgggt atcggagtcg
     1441 ctgattcgtc atgaataccc cacatcttta acttctctta aatccctgag ccctcaggat
     1501 ctttaccaac agctttgtca ttaccgtgtg gcccagcaat tgcgtcaggc gttaactcat
     1561 cccggacggc aggcagtacg tttgagtgag ttgctcaatg ttgccagaag aacctggcca
     1621 gatattaatc accgtgagct actgcaactc ctcgatctaa tggcaagatc gcgagaaggt
     1681 gaactggctt ttacgccatt acgtatgcat gggtttatca gaacactggc gggtctatgg
     1741 gcatgttcaa acaaacaatg ctcacataaa gcacatgaat taaatcagag tgactggcca
     1801 tttggtcagg tatggtttga gcagcgccaa tattgtgact gtggagcgcc ggtatttgaa
     1861 gttttacgtt gctccgggtg tggttcagct tatttgtctg ccaaagagga gatgcgcggt
     1921 gatggaacga actggttggt agcgcaacca gccactgccg aagtggatga gtttgctctt
     1981 gatgtagatg tttattctga agatgaagac aacgacgaat tagacaataa ctctcaattt
     2041 gatcgtctga ttgcgagcga tggtgaaaaa tatatcattt cactggaaga aacgacggcg
     2101 gggaaaattg atagtgaagc gcaaaagcac tatgaaataa acctgttaag accggaatct
     2161 cgaggtgagg gacgcaataa ctcctttgct tgtgtttgct gtggtgacac tcaacggaaa
     2221 aataatcctt tattccgtcc gcttcggctc ggcgcgccct tttttttaaa tgaaattatt
     2281 cctactctgc tggagttttc tccattaccg caaacaagag aagagcagtt aggaccgttt
     2341 aatggtcgac gcttgctcac ctttacggat tcgcgtcagg gtaccgctcg catttcagcc
     2401 cgcttacagc aggatgccga taaagcgacg ttgcgagctc tttgttacca ggagctggca
     2461 aatattacag agccttcagc aaaactcagc ccggcacact gtgcactgtt agagaaagtt
     2521 attagccagt tttctgtcct ccctttaaaa aattccatcg acgcgctgaa aaaagcgatg
     2581 gctgcgaatg aggagcttag cccgacggac attcaggcac ttaaaattat tgagccgatg
     2641 gtgagttcct taagcacgga gcatgctgaa gcgatgcaaa tacttcaggc taactctggc
     2701 aatcagcaga agaaaaccat gtcatggcaa acgctgtctg aacagctggt tggcagtgtg
     2761 gaccttgccg atagaatgag aggcagtttt aaagaattgt ctgggcttga tcttactaaa
     2821 caacagtttg cagatttttg tttatacagc gagtttggtc gccgccctaa aaatgcctgg
     2881 accctcgaaa gtctgggtct ggtagccatt cactatccat tcattgataa agttacaacc
     2941 tgcccgcagg actggaagac attattaccc gatcccgata agcaattagc ggaatggcgt
     3001 aatttattga agattactct tgatttcttt atccgtgaaa acagtgccgt tttttacgaa
     3061 aacgagcaat accctcgctg gatgggggca cgtttcccgg taaaaatgct ccaggggcca
     3121 gataaaaaaa acaaagggca gaaacgtgat caactttggc cacaaatacg cgatcgccat
     3181 tataaccggg tcattaagct attaatcgca gtctttccgg caatacaacc tgagcaggct
     3241 cactggaaat cgttagttaa ccacttaatg attgaggtct gggatgcaat tcgaccctgc
     3301 ttacgtcagt ttgagtcggg ttatcagctg gatattaaac aacaagctga attttacagt
     3361 ccaaagcaag tgtggcgttg cccctatact cgacgtgcgt tagacgtcac gttgctggga
     3421 tactctccgt atttgccggg tagcaaagaa attgcacctg aaaaagcagt gttgattgaa
     3481 atgcctgagc ttcctgtacg tcactggcgt ttgtctggtg gtggagaaat cgcccgagaa
     3541 gaacgtctgg agtggctgga aagtaatgcg ctgattcaac atgcccgcga ggaaggatta
     3601 tggtctactc gcagcgaccg actggccctg aaagatagct ggtatcgtct ggaggaacac
     3661 tctgcgcaga gaacgcctga gcagaaccag tttaatgaaa aacaatttaa gtcaggcaaa
     3721 gttaatgtgc tgaactgttc cacgaccatg gagatggggg tggatattgg tggtatgagt
     3781 ttggtggcaa tgaacaacgt tcctccagcg ccggcaaatt atttgcaacg tgcaggtcgc
     3841 gcggggcgtc gtggagagag tgcatctgct gccattacgt tatgtaaaaa cacggcgcat
     3901 ggaatggagg tattcaaaga ccccctctgg gcgttcaaca ctactgccag tgcgccacgg
     3961 gttcgttttg ggagcagtag tattgttcaa cgacatgtta atgccctggt tttaggtctg
     4021 tttttacgag ctgaagttcc tgatgcgaca aaactgagtt gcaaatggtt ctttgaaggt
     4081 gatgagagcc agtgtcttcg ttttctccat tggttaaatc accaggccga tcagctggcg
     4141 gataaactta aacgccttac tcagggaacc gtactgatgt cattgacggc aacccagctc
     4201 ttgacccgca cacaagcaat gatgcagcag gttgatatcc gctggagaag ccaacttgcg
     4261 atactgttgg aaaatatcga agcgttgaag gcggataaca gcgcgtggga agaaacacca
     4321 gcaggtaaag ccatcgctta tcagcttcgt gattatcgcg gtgcatatct gttttctaaa
     4381 ctcattagtg aagcgttcct gccggggcat ggtttcccgg ttggcgtagt caatttcaat
     4441 tatctgacgg cagatgagct ggaaaaacgt cgcgcaataa aagccactca ggctgaccct
     4501 aatgaagggg gagaaagctt ttctcgtcgt attgaaaaat tgcccagccg tgatttgcct
     4561 acagcattac gcgagtatgc gcctggtgca gatgtggtat tgggcggaaa agtatatcgt
     4621 tcctcgggca ttatgcttgg caaggtgctt gccagcgggc aggagctgtc aggcgatcat
     4681 catattccgt ggttctggca ttgccgtaaa tgtggtgcgg gggcaaccag tactacccat
     4741 cctgtcgaat gtagtcactg caaagcggat atacagcaac ttgatgtgaa gcgttattta
     4801 cagcctgttg gttttgcgac agatattcgt tatcaggcac ataatgatgt atccatgcct
     4861 gcacaacttc cctggaaaga tccacgtgtt cttgtaccgt cttcggtgtg ggtttcttta
     4921 cccgatgctg ggctaggacg ttaccgtttt agccatagtg gggaattgtt ccacttcagt
     4981 gaaggggaat ttgggcacgg ctacgcaatt tgcctctcat gtggtcgggc agaatctcaa
     5041 actcaacccc agcgtacccc tgaaaatcta aagaacccag agagggaaaa tacgcattac
     5101 ctgttgcgag ggggcagtaa cgatcgtcag gggagcaata aattgtgcca cggtcatgtg
     5161 cataaagatc tgtggttggg atatagcagt cgtacagata tggttgagct gcaactcaat
     5221 gatgataatg gcctgttgat ccgtgatgag gttgccgcgc gttcccttgc ggtcgctttg
     5281 cgtgaagggt tagcgcataa attgggtatc gaaaatactg agctgggcgt gacgacgcag
     5341 caagcgcgcg atatcaatgg ctataccgga tattccatct tcatttacga caataatgcg
     5401 ggtggtgcag gttatgcagt gcaattgatc gaccattggg ctgatgtctt taattacgcc
     5461 agaaaattgc tcgattgcag ttgcgataaa ttctgtcacc actgcttgct cagctacgac
     5521 agtcagcatt atgtcaacag attagatcgt caccatgcat taaccctgct taccaatgta
     5581 cggttgcagc gacttaacct cgcgccagaa tatcagtact tcggcgatgg tagccgggtt
     5641 gaaactaacc ctctgagttt acgtattgcg caatgcctga acagcgagat ttatgactcc
     5701 tgctcgctgg ttcttgcggg gccgcaggaa cagtgggact ttgcccaatg gccattattt
     5761 aaagagttgc tccagtttgc ctcctccggt gggaatgttg agttgctggt ggccacgccg
     5821 ctggcaaact taactgatag ctccagacat cagctttccg cgttggcggc gatgccggga
     5881 ggtcgcttac aagtgaaatc cattgccact gcgcagctca tgcaaggaaa ggggcgctgg
     5941 cttgcgcagg tgactcgcga agggcaaagc caacagtggg ccgcagacga cagcgctacc
     6001 gttgcacctg gtgaattatg gggacaaagt gcgtcttcac cagtagtgac acttaaagga
     6061 acgtccggaa aaaccttctc gggtcagacc ttaagtgcag aagatttact gcccgcctta
     6121 cccaccgggg cggtgcgcat caatctctgt gaacaactgg acgggccgct ggaaggtttt
     6181 ggttcgcgtt tctggtcgtt agtcacgcaa cagcacgctg gctggaagca ggcatttacc
     6241 cgccacaaag agataacgca tgttgagtac agcgatcgtt atcttaatag cccattcacg
     6301 gcgcgtttgt taggtgaaat cttaaccgag ctggttgaac agggaatggc ggaacgcgcc
     6361 agcctcactg tttgcgtcaa aaaattggat tacaactctc gccaacacga cgctctctat
     6421 aacgcgtggc ttaatgagga agatcgtcag caggttgtga ccactttact tgaagaagga
     6481 tatctggggc ctgcgtggcc gggagcaatt agctggttaa cgggggataa ccagagtacg
     6541 gagcatggac gagaactgac agtcaccttc agcgatggca gtcagcatta tgtcctgctc
     6601 gacatggggc ttagctactg gcgctgtatt gaagatacct tctttgattt tgccctgcga
     6661 gtaccgcagc aagttgaacg gctggcaaac acgcgagcgc gagctgttgc cccgggtaat
     6721 gaccttagga gttatattat cgctggataa ataattgctc ccctggggat atcttccagg
     6781 ggagttgacc cagctcactt tttaataatt tttgtttgaa aatcatgcgc aatttatgcg
     6841 cattccctcc cattttgtca ctttgccggg ggccttcatg atatctttaa ggccctcttt
     6901 tttatcgcag tgtgccgcgc cacatttttc aggatgacgt taccgccact gccccaaata
     6961 gacagaaaaa ccgatgaata agcaaaatta tgcacctggt atgcgggtgg ttattcgtga
     7021 tgccgaatgg cgtattcgcc gggcggatga cagtggtgat ggtgggtatt tgctgacctg
     7081 cgatggtatt tcagagcttg tgcgcggtaa agaagggtta tttctgacca aactggaaca
     7141 aaaagtagag atcctggatc ccgcaaaaac gcatttggtg gaagatgaat ccgccaatta
     7201 tcaagcggca cagttgtata ttgaaagtca gttacgccaa cgcgtaccga cagacagtaa
     7261 agtccatttt ggtcatctgg cggcgatgga ttccatgccg tttcagctcg atcctacgcg
     7321 gatggcgctg gcacaaccgc gtcagcggat actcattgcc gacgctgttg gtctgggtaa
     7381 aacgctggaa gccggtattc tggtttcgga actgatccgt cgtgggcgcg gaaaacgtat
     7441 tctggttctg gcggttaaat cgatgctgac acaattccag aaggagttct ggagccgttt
     7501 tgcgatccca ttgacccgcc tggattctgc tggtttacaa aaggtgcgta accgcatccc
     7561 aaccaaccac aacccgttcc actattttga caagaccatt atctccatcg atacgctgaa
     7621 acaagacatt gaatatcgtc accatctgga aaatgcctgg tgggatatta ttgtcattga
     7681 tgaggcccat aacgtcgcag aacgtggaac cagttcgtta cgcagtaaac tggcaaaact
     7741 gctcgccggg cgcagcgata cgctgattat gctttcagca acaccgcatg atggtaaggc
     7801 tgaaagtttt gccagcctga tgaacatgct tgacccgacg gcgatcgcca acccgaaaga
     7861 gtatgaatac gccgattttg ccgataaaaa tctggttgtc cgtcgcttca aaaaagacgt
     7921 gaaagatcag atgtccggcg agttccccga gcgcaatatt gtcaaattga ctcgcctggc
     7981 atccggtgcc gaagaagaag cataccgtcg tctggttgag agccagttcc gtgatgatga
     8041 cgatgaacag gcccagtcta acaaaggtcg tctgttcaag ataaccctcg aaaaagcgct
     8101 attttccagc ccgatggcgt gtgccagcgt cgtggcgaat cgtctgaagc gtcttgagag
     8161 ccgtaaagat cataatagcc agagccagat caacgaactg gagtcattgc tgttggcgct
     8221 gaataacatt gatgccagcc agtttagcaa ataccagttg ctgctcgaca ccatccgaaa
     8281 agacctcgcc tggaaagcca ataacacgga agatcgcctg gtgatcttca ccgaaagtat
     8341 taaaacgctg gagttcctcg aacagcaact gcgagcggat ctgaaattga aagatgacca
     8401 gatcgccacg ctgcgcggcg atcagggcga tacggtattg atggaaaccg tagaagcctt
     8461 tggtaaaacg cagtcgccgt tgcgtcttct ggtttgttcg gatgtggcat ctgaagggat
     8521 caacctgcat caccttagcc acaaaatgat tcactttgat attccgtggt cgttgatggt
     8581 attccagcaa cgtaacgggc gtattgaccg ctatgggcaa aaacatcagc cccaaatccg
     8641 ctatctgctt accgaggcca gcgaaccaca gatcaatggc gatatgcgcg tactggaagt
     8701 gttgatcaac aaagacgaac aggcgcagaa gaacattggg gacagttcgg agtttaccgg
     8761 caaatttacc caggaagagg aagaagagca ggttgcagag ttcatgatgc aggatgatgg
     8821 tgccagcctg tttgatcaac tgctgaacag caacgtctcg gaaagcgccg aacacgatct
     8881 gtttggcgaa atatgcagtg cggtttccag cgatgcctca atggtcaccg aaacagacac
     8941 cagcttgttt gccagcgaac aggcgtactg cgaaagagca ttgggttacc tgaaagccag
     9001 tggtcaaact atccagtatg aaaccttgcc tgataatacc ttgtcgctgg tggcaccgga
     9061 ggagttacgc cgccgcttca accagctacc gcctgaaatt gccccggaga actggcagct
     9121 ctatttaagt caggataaaa ccgtcatcac cgacgcgatt gcgcgcgctc gcggtgagca
     9181 acatgcctgg cccgatgtgc agtatctctg gcaaatcaac ccggtagtgc agtggctgga
     9241 cgataaaatc tcttcggctt ttggtcgtca tcaggctccg gttatccggc tgccatacct
     9301 gcttgaaccc gatgaagatc acttcattct ttccgggtta ttcccgaacc gtaaatcaca
     9361 tccgatggtg aacccgtgga tagtggtgag ctttaaccgt gaatcgctaa tcggcagtca
     9421 gcctttcgct gagtttttac aacgtcatcc gcagttgagt aacaagctga ctaacagcgg
     9481 cggtaaagat cgtaatcacc aacgccagca ggatttactg gaagcggcta ttgctcatgc
     9541 ccgggaggta ttcatccatg atcggaatgc gtttgaaacg cacattaacc agcaactgaa
     9601 tgagcatctg caaaagctgg acgttttgcg tgggcggcag ttgagccaac ttgagctgga
     9661 ttttgccgat aacaaacagc aattgtcagt caagcagagc cgtaaagagc agagacaacg
     9721 cgaaatcgaa cacaattttg acagctatat cgaatggatt gaggacacca tgacgactga
     9781 aaaagaaccc tacattcagg taattgctgt tatcaccgga gcggagggtt aatcatggcg
     9841 ctggtcggta ttaataacga aaacgaattt tactctaacc actatttggg tgaggttttc
     9901 accagtgata tccgcgatgt gctggaaccc tggatagccc aggaaaatgc agcgcgtgaa
     9961 gcggagcgtg ccgctcgtga acagggcaaa gacgtggagc cgggataccg cgctccgtgg
    10021 aaccagttta acagtctggc gactgagttt ttccgcaaac ttgccgagca cgaaaaacag
    10081 cgtcagatcc cgcagcgtct ggccgatcaa cgtaatcgct ggcagccatt gttaaaggcg
    10141 ctgggctacg aaattacgcc acagatccaa atgctggatg acgatacacc gctgccggta
    10201 ctggcgcgtt acaacagcac tgacggtagc ccgtggctgt ggattgttga agcacacgat
    10261 caggaagaag gaacgctgga tccgctggcg ctctccttac tgaccgcgca attcccggcg
    10321 gataccgaca aacataagca cgacagcctg cgcaaaaaag ccaacggtga atatcgcagc
    10381 tggcaggatt tgctctctac ggcggtcttc acccaaaatg aaccgccgcg ttttgtgctg
    10441 ctgctcggta accgtcagct attgttgttg gaccgtacta agtgggcgca aaaccgtctg
    10501 ctacgttttg attttgaaga gattttaagc cgtcgtgaaa cggatacgct gaaagcgact
    10561 gcggtgttgt tacataaaga ttctctgctg ccgggcagtg gggcacctta tcttgactcg
    10621 ctggatgaca attcgcacaa acatgcgttt ggtgtttcgg aagatctgaa atatgccctg
    10681 cgcgaaagca ttgagttgct gggcaacgaa gcgatgcatt atctgatcga ccgtggcctg
    10741 gcaaactata caggtaatcg tgcggtggac ccggatgaac tgagccgcga atgtctgcgt
    10801 tacatgtacc gcctgctgtt cctgttctac attgaagcgc gcccggagct gggttatgcg
    10861 ccaatgaccg ccaaaaccta tctgcaaggt tacagcctgg aaacgttgcg cgatctggag
    10921 atgatcccgc tgaccagcga agaagatcgc aacgggcgct acttccacga cagcctgaat
    10981 atgctgttta aactggtgcg cgaaggctac aacggcggcg tgaaaatgca gagtgacctg
    11041 gagagcggcg accggatcac catccatagt catcagttca gcgtcccgcg tctggaaagt
    11101 catctgtttg atgccaacaa cacgcgcatt cttaaccgcg tggtattccg taacgaaacc
    11161 ctgcaacaga ttatccaggc gatgtcgtta agccgcccgg ccaaagggcg ctttaaccgc
    11221 cgcggacgta tttcttatcg ccagttgggt atcaaccagt tgggtgcggt gtatgaggcg
    11281 ctgctctcct atcgcggatt cttcgccagc gaagatctct acgaggtgaa gaaagccggg
    11341 gaagagttta acgagctgga gacgggttac ttcgtcagta aggatgagat tagcaaatac
    11401 cacgaagacg agaaggtcta cgagaaagac ggcagtctgc gcattcaccg caaaggcagc
    11461 tttatctacc gtatggccgg gcgcgaccgt gagaaatctg cctcttatta caccccggaa
    11521 gtgctgaccc gctcactggt taaatatgcc ctgaaagaac tgtttaagga gcagattgat
    11581 cccattagcg atccgcacgc caaagctgat gccatcttaa acctcaccgt gtgcgaaccg
    11641 gcgatgggca gcgcggcgtt ccttaacgaa gccatcaacc agctggcgga agcgtatctg
    11701 ttccacaagc agcaggcgga aggtcgccgt attccgcagg atcgttacac ccaggagtta
    11761 cagcgggtga aaatgtacat tgccgacaac aacgttttcg gcgtggactt aaacccggtg
    11821 gcggtggaac tggcggaagt gtcgctgtgg ctgaacgcca ttagtggcga tgcgtttgta
    11881 ccgtggtttg gttaccagct gcactgcggt aactcgctgg tgggcgcgcg ccgtcaggtg
    11941 ttcaacaaga gcgaactgac ctacaaaaaa gccaaagatc cgagctggct taacagcgag
    12001 ccggtcgaac tggcgatgaa cacgccgcgt gaagagacgc agattttcca cttcctgctg
    12061 cccgacggcg gtatggctaa ctacagcgat aaaactgtta agcagcgtta tccggatgac
    12121 ttcaaagcgc tggacagctg gcgcaaagag tttattaaaa gctttgccgg gcatgagatt
    12181 gctgatgtgc agcgtatcag cgaaaaggtg gaagcactgt ggaacaccta tcgccagcaa
    12241 cttaaagcag aacgtctgaa aaccgccgac agctacccgg tgtggccggc agaaaacagc
    12301 gagcagacgc gttcttcgct gagcagtaaa gatgaaacct ttagcggtcg tcttgaagat
    12361 aacagcgcct accagaagct gcgttgggta atggactact ggtgcgcgct gtggttctgg
    12421 ccgatcgaca aagccgatga gctaccggat cgcggcacct ggttgtttga gattgaaacc
    12481 ctgcttgacg ggattgtaat cacggaaaaa gtcactgaag ttgcggagca caccaccggc
    12541 gatctgtttg ccgaagaagg cctgctgcga gaagagtctt cgctgttttc tgttgctggt
    12601 cgtctgaaaa ccgaggtgtt gttccgtcat ttgccgcgtc tggcgattgt cgatgccctg
    12661 agaaagcagc accgtttctt ccactgggat ctggagttct gcgacctgtt tgccgagcgc
    12721 ggcggttttg acctgatgct cggaaacccg ccgtggctga aagtggaatg gcaggaagcg
    12781 ggcgtgctgg gtgattacga gccggaattt gtgctgcgta agctgagcgc ctcgaagctg
    12841 gcaacgttgc gtattgatac ctttaaccag atcccggcgc tggaagcggc ctggcgcagc
    12901 gagtatgaag gctgtgaagg gatgcaaaac ttcctgaatg cgcagcagaa ctacccggta
    12961 ctgcgcgggg tgcagaccaa cttgtataaa tgctttctgc cgcaggcctg gcgattaggg
    13021 gcgcagaaag gcgtggcagg tttcctgcac ccggaaggga tttatgatga cccgaaaggc
    13081 gggcaattac gtgcggcggt atatccgagg ctgagggcgc attttcagtt tcagaatgag
    13141 ttaaatttgt ttgttgaagt tgatcaccat gcgaagttta gcagcaatat ttattctgct
    13201 agccctagca cagtgggatt tgaacatata tctaatttgt atgctccgca aactattgat
    13261 gcatgttttg aacattctgg cagtggggac attcccggtc tcaaagacga gattgagagc
    13321 gagggaaaat taaaagttgt atggaacaca tctggccacc gttctcgatt aataagtatc
    13381 gccactcatg agctagaatt atttgctcgt ctatatgaca gcgaaggaac gccagcctgg
    13441 caggcacgtt tgccagcctt acatgctaaa caacttgttg ctgtactgga aaagtttgct
    13501 aatcagccga atagattagg tgatttgcag gggcagtatt tttcaacggt tatgttcgat
    13561 gaaacatatg ctcagaggga tgggacaatt ttacggcaga ctcaattccc tcaagattca
    13621 tcacaatggg tactgtctgg ccctcatttc tttgttggga cgccgttcta caagactccg
    13681 cgcgaaaact gtacgcttaa cagcgattat gactgcctgg acttgctaac tctgcctgac
    13741 gactatctgc cgcgcactaa ctacattccg gcatgtgatg cacaggagta tgcaaaacgt
    13801 actccatgcg ttacatggac tgaactggct gaagatgaac cgaagaaggt aacagattat
    13861 tatcgcttag ctatcagagc catgttggct caatcggggg aacgtacact aattagtgct
    13921 atttatccgc cagaaataag tcacatgaac gcagtacgtt cttactgcta tagctcacag
    13981 aatctgttac tcgaacattc aggtatgtgt ttttctttac cttttgattt tatttg
//
LOCUS       DBFIFI010000108        13955 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.108, whole genome shotgun sequence.
ACCESSION   DBFIFI010000108 DBFIFI010000000
VERSION     DBFIFI010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13955
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.108"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            137..646
                     /locus_tag="N7B39_003041"
     CDS             137..646
                     /locus_tag="N7B39_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HGU2422984.1"
                     /translation="MNQQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIESTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPYAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            643..2061
                     /gene="phrB"
                     /locus_tag="N7B39_003042"
     CDS             643..2061
                     /gene="phrB"
                     /locus_tag="N7B39_003042"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HGU2422985.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARLLALYIATPRQWAA
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFAASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVQVERTLRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLNEL
                     IWREFYRHLMTYYPSLCKHCPFIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRQWLPELRNVPGKSVHEPWKWAEKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(2211..3692)
                     /gene="dtpD"
                     /locus_tag="N7B39_003043"
     CDS             complement(2211..3692)
                     /gene="dtpD"
                     /locus_tag="N7B39_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HGU2422986.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELRLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGVVLMIWLYQALKFRNRALALES"
     gene            3963..4706
                     /gene="ybgI"
                     /locus_tag="N7B39_003044"
     CDS             3963..4706
                     /gene="ybgI"
                     /locus_tag="N7B39_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HGU2422987.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            4729..5385
                     /gene="pxpB"
                     /locus_tag="N7B39_003045"
     CDS             4729..5385
                     /gene="pxpB"
                     /locus_tag="N7B39_003045"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HGU2422988.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            5379..6311
                     /gene="pxpC"
                     /locus_tag="N7B39_003046"
     CDS             5379..6311
                     /gene="pxpC"
                     /locus_tag="N7B39_003046"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HGU2422989.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKAKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKSRQDQQRYFEQLAWRLHNEN"
     gene            6301..7035
                     /gene="pxpA"
                     /locus_tag="N7B39_003047"
     CDS             6301..7035
                     /gene="pxpA"
                     /locus_tag="N7B39_003047"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HGU2422990.1"
                     /translation="MKIDLNADLGEGCTSDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRKNFGRSAMQLPPETVYAQTLYQIGALATITRAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQSGALIEDEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSAFAEKGIVVAA"
     gene            7071..7861
                     /gene="nei"
                     /locus_tag="N7B39_003048"
                     /pseudo
     CDS             7071..7861
                     /gene="nei"
                     /locus_tag="N7B39_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308766.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
     gene            complement(7858..8904)
                     /locus_tag="N7B39_003049"
     CDS             complement(7858..8904)
                     /locus_tag="N7B39_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HGU2422991.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLGLHLPAALLLGPMIAGIIFSMRG
                     VTLQLPRSAFLAAQAILGCMIAQNLTGSILTTLEVNWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQHIVWFPPVSINLLLTILLAVVAGTVGCLLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRTA"
     gene            complement(9053..10114)
                     /locus_tag="N7B39_003050"
     CDS             complement(9053..10114)
                     /locus_tag="N7B39_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2422992.1"
                     /translation="MRFAKGVLLAICLIFLPLKAALALNCYFGTANGAVEKSEAIMPF
                     AAPANSKPGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGIQDPYYQLGVTY
                     EGVDYDASLGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKLCSGDPTVMHKSRTFVA
                     RMRLYVKVRAMPPHDYQSKLSDYIVVQFDGAGSVNEDPTAKNLKYHITGLENIRVLDC
                     SVNFAISPETQVVDFGRFNVLDIRRHTMSQQFKITTTKSQNDQCTDGFKVSSSFYTDE
                     TLIDEDKSLLIGNGLKLRLLDENASPYTFNKYSEYADFTSDLLVYEKTYTAELSSTPG
                     TPIDVGPFDTVVLFKINYN"
     gene            complement(10111..10842)
                     /locus_tag="N7B39_003051"
     CDS             complement(10111..10842)
                     /locus_tag="N7B39_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HGU2422993.1"
                     /translation="MTFVKGFPLILLVASMCSHGAVQPDRTRIIFNSKDKATSLRVEN
                     RSDKLPYLAYSWIENEKGEKSDEFLVALPPIQRLEPKATSQVRIMKQAATAKLPTDRE
                     SLFYYNLREIPPVPEGSEGHAILQVAVQSRIKLFWRPAALRKKMGDHVEQQLQVSQQN
                     NQLTLKTPTGYYLTIAYLGRDEKGVLPGFKSTMVAPFSSVTTSTGSYSGKQFYLGYMD
                     DYGALRMNTLSCQRQCRLQPVENKK"
     gene            complement(10857..13316)
                     /locus_tag="N7B39_003052"
     CDS             complement(10857..13316)
                     /locus_tag="N7B39_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HGU2422994.1"
                     /translation="MDAVNIYRLSVLSCLAMVTPPALTAEFNLNVLDKSIRDSVDISL
                     LNQKGVVAPGDYFVSVTVNNNKISNGQQIRWQKSGDKIIPCINESLIELFGLKSDFRK
                     KLPAIKECVDFSVFPEIIFTFDQANQQLNITIPQAWLAWHSENWTPPSTWNNGIPGFL
                     MDYNLFASTYRPQSGSSSNNLNAYGTTGLNAGAWRLRSDYQLSQSDSGDNREQSGAIS
                     RTYLFRPLPQIGSRLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISHSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVSEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGRPRSSMSHHTEDETFISHEVSWGMLSNTSLYGGMLLAGDD
                     YRSGALGIGQNMLWMGALSFDVTWADSHFDTQQDEQGYSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYIDHKYNDADAQDEKQTISLSFGQPITLLNLNLYANILHQSWWN
                     ADTSTTANITVGFNVDIGDWKDISVSTSFNTTHYEDKDRDNQIYFSISLPIGESGRLG
                     YDMQNNSNTTTHRMSWNDTLDERNSWGMSAGIQSDRPDNGAQVSGNYQHLSSAGEWDL
                     TGTYAANDYTSASASWSGSFTATQHGAAFHRRSSTNEPRLMVSTDGVGDIPIQGNIDY
                     TNRFGIAVVPFVSSYQPTTVAVNMNDLPDGVTVSENVVKETWTEGAIGFKSLASRAGK
                     DLNVIISDANGHFPPLGADVRQAEGGVSVGMVGENGHAWLSGVDENQQFTVHWGDQKT
                     CAIHLPEHLEDVTKRLILPCH"
     gene            complement(13366..13932)
                     /locus_tag="N7B39_003053"
     CDS             complement(13366..13932)
                     /locus_tag="N7B39_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2422995.1"
                     /translation="MFKGQKTLAALAISLLFASPVFAADEGSGEIHFKGEVIEAPCEI
                     HPDDLDMNIDLGEVTTSHINREHHSEKKPVNIRLINCDIAGWDDGKGGVVSKVGVTFD
                     STAKTTGATPLLSNISAGEATGVGVRLMNKDESFVTLGTEAPTIDLLASSTEQTLNFF
                     AWMEQMDNAVSVTAGAVTANATYVLDYQ"
BASE COUNT         3379 a         3457 c         3799 g         3320 t
ORIGIN      
        1 attcgctgct ttgatgatga tgtaaaatga ttgtttttga actggatata acagcttggt
       61 agggaagaaa gcataacccc acaaaattca tttaccatcg tctacactta ctcctgtaaa
      121 ccgttcagga gcagtaatga atcagcaacg atttgatgac agcactctaa tccgtatctt
      181 cgcgcttcat gagttacatc gactgaaaga acatggctta acgcgcgggg cgcttctcga
      241 ttatcacagc cgctataaac tcgtatttct ggctcattcc cagccggaat accgcaaact
      301 tggcccgttc gtggctgata ttcaccagtg gcaaaatctg gatgattttt acaaccagta
      361 ccgccaacgc gtaattgttt tgctttctca tcccgccaac ccgcgcgatc acaccaacgt
      421 tttgatgcac gttcagggtt attttcgccc gcatattgag tccacagaac gccagcagct
      481 ggctgcgctt atcgacagtt atcgccgtgg cgagcaaccg cttcttgcgc cgctgatgcg
      541 tatcaaacac tatatggcgc tttatcctta cgcctggctt tcagggcagc gttatttcga
      601 actttggccg cgtgtgatta acttgcgcca ttcaggagtt ttatgactac ccatctggtc
      661 tggtttcgcc aggatttacg tctgcatgat aatctcgcgc tggctgccgc ctgccgcaat
      721 tcatctgcac gcctgctggc gttgtatatc gctacaccac gccagtgggc ggcgcataat
      781 atgtcgccgc gtcaggctga acttatcaat gctcaactga acgggctaca aatcgcgctg
      841 gcggaaaaag gcattccttt attgttccgt gaagtggatg actttgccgc cagcgtcgaa
      901 atagttaaac aggtgtgcgc ggaaaacagc gttactcatc ttttttataa ctatcagtat
      961 gaggtgaatg agcgggcgcg ggatgtgcag gttgagagaa ctctgcgtaa cgtggtgtgt
     1021 gaaggatttg atgacagcgt gatccttccg cctggcgcgg tgatgaccgg caatcatgag
     1081 atgtacaaag tctttacgcc ttttaagaat gcctggctga aacggctgcg ggaagggatg
     1141 ccggagtgcg tcgctgcacc aaaagttcgt agtagcggat cgataaagcc cgcgccatcc
     1201 attacgctga attatcctcg tcagtctttc gatactgcgc atttcccggt ggaagaaaaa
     1261 gcggcgattg cgcaattacg ccagttttgc cagaacggtg ccggagaata tgagcaacaa
     1321 cgagattttc cggcagtgga aggcaccagt cgtttgtccg ccagcctggc aacgggcggg
     1381 ttatcgcctc gccagtgttt gcatcgcttg ttggcggaac agccgcaggc gctggacggt
     1441 ggggccggta gtgtctggct taatgagctg atctggcgcg agttctaccg tcatctgatg
     1501 acgtattacc cctcgttgtg taaacattgt ccgtttattg cctggacgga tcgtgtgcag
     1561 tggcagagca atcccgcaca tttacaggcc tggcaggaag gcaaaacggg atacccgatt
     1621 gtcgatgctg ccatgcgtca gcttaacagc actggctgga tgcataacag gctacggatg
     1681 attacagcca gttttctggt gaaagatttg ttgatcgact ggcgcgaagg cgagcgatat
     1741 ttcatgtcgc agctgattga tggtgatttg gcagccaata acggtggctg gcagtgggcg
     1801 gcttctacgg gtactgatgc agcgccgtat tttcgtattt tcaacccgac aacccagggc
     1861 gagaaatttg accgcgaagg cgagtttatc cgccagtggc taccggaact gcgcaatgta
     1921 ccggggaaat cggtgcatga gccgtggaag tgggctgaga aagcaggtgt gaagctggat
     1981 tatccgcaac cgatagtcga gcacaaagag gcgagagtac aaacgttggc agcgtatgag
     2041 gcggcgcgga aggggaaata acagacgcgt caggcaatcg agcccagatg ccggatgcgg
     2101 cgtgaacgcc ttatccagcc tacaaatttg tgcaaattca ataaattgca ggaaatacgt
     2161 aggcctgata agacgcgtca agcgtcgcat caggcatcgg tgctggccta ttaagactcc
     2221 agcgccagcg cgcggtttct gaatttcagc gcctgataca gccaaatcat cagtaccact
     2281 cctacacacg ccagtgcgcc ccaggtaatt tgatcaaata cttcaatata tgcattgatg
     2341 gagtagttga tcgccccgga agcatcaaac gaagcctgcg atgtctgatc ggcaatcacg
     2401 cccgccagat agttcgcaat cgcgccggaa agcagcatat aaatgccggt taatacgccg
     2461 gtcacaccgg ggatttcaat gcgcgtaatc tgcgacatag caaccgggtc gataaacagt
     2521 tccgcaaagc ccatcaccgc caggcctaat accatcagtg gcagagaaga gtgaccatac
     2581 attgcggacc agcgggcgct taaggtcaga atgcagaatc cggcgctcat caggccaagg
     2641 ccaagagcaa attttcccca gatgcgcacg gtacgattac ccgcgacact ctcttttacc
     2701 acccacgcca ggaacacacc gcacagcata actgcgaagg cattaatcga ctggaacatc
     2761 gcggtcggaa cggtataacc aaacatatcg cgattaacga agcggtcgat ataaaggcta
     2821 atcgagctac cgccctgttg tgcgaaggcc cagaacaaca tactgaagaa ggtgagcgtc
     2881 acaatcagcc tcagctcctt ccgctgtttc tggttttctg ctttgcgata aatttttgcc
     2941 agtacaccca ggccaatgat agtcgcgaca atcaaggcgt ataccgacca ttctttccag
     3001 aacagtacgg taatcagcgc aggcgttgcc accagcagaa ccagcagcca tccccagttc
     3061 ggcagcagaa agtttgtcgc acgcagtact tttttgttaa cgccgcgggt atgtgtgaaa
     3121 tgacgattgc cacataagaa aataaccaga cccgcgatca tacccaccgc cgccaggcca
     3181 aagcccatcg cccaactgta ctcttcctgg gcgtagccac aggcaatagg tgcgataata
     3241 gaccccacgt tacccgctgc atacatcagc gagaaaccgc catcacgacg cggatcggtt
     3301 ggctcataca gctcgccgag caggcaactg acattggatt taaacagacc atagccacaa
     3361 acgattatcg ccagggacag atagaggaat gacggatgga tctcacttgc acccaacacc
     3421 acatgaccga tcgccatcaa taacgccccc agcatcaccg ccatgcgatt gccgagaact
     3481 ttatccgcca aaaagccacc gaggattggc gtgacataca ccagcgaaca gtaggcgcta
     3541 aataactcgt aggcgtgagt atcgttgtat tttagttgat tggtgagata gagaatcagc
     3601 agggcacgca tgccgtaaaa gctaaaatat tcccagattt gcagcgcaac gacatagtaa
     3661 atagcgcgcg gttgtgatgc gtgtttattc ataataattc cacaattgac ggcaacgtca
     3721 cgtaatagtt gcgcggcgtg cttgtgtttc cttaaagtta aaactttgat acagatctga
     3781 ttatttatgc aatatgctgt ctgattgcat aaatatacat tagctgaagc gtgatgatat
     3841 aaacaatttg cgtcttttgg cgtcgggttt gtttcttaat atgtcaaaag attggctatc
     3901 gacgaacagg cgaggtaacg ctatgttaac ggtgtagcta tcagaatacg gaaggcgcga
     3961 agatgaaaaa caccgaactg gaacaactga ttaacgaaaa actgaacagc gcggcgatta
     4021 gtgattacgc gccgaatggt ttgcaggtgg aaggcaaaga gacggtgcaa aaaattgtta
     4081 ccggtgtcac tgccagccag gctttgctcg atgaggcagt gcgtctgggc gcggatgcgg
     4141 tcattgtgca tcatggctac ttctggaaag gcgaatctcc ggtcattcgc ggcatgaagc
     4201 gtaaccgttt aaaaacgttg ctggcgaatg atatcaacct gtatggctgg catttgccgc
     4261 ttgacgcaca tcctgagctg ggcaataacg cacagctggc ggcgttactg gggatcacgg
     4321 tcatgggcga aattgagccg ttggtgccgt ggggcgaact gaccatgcct gtgccgggac
     4381 tggagctggc ttcctggatt gaagcgcgtc tgggacgtaa gccgttatgg tgtggcgata
     4441 ccggacctga ggtggtccag cgcgtcgcct ggtgcacggg cggcgggcaa agttttatcg
     4501 atagcgccgc gcgttttggc gtggatgctt ttattactgg cgaagtttct gaacagacca
     4561 ttcattcagc ccgcgagcag ggattgcatt tttatgctgc aggtcaccat gccactgaac
     4621 gtggtggtat tcgcgcattg agcgagtggc tgaatgaaaa taccgatctt gatgtgacct
     4681 ttattgatat tcctaatcct gcataacgaa taatcagagg gatcgaaagt gcaacgagcg
     4741 cgttgttatc tgataggtga aacggcggta gtgctggaac tggaaccgcc ggtgacgctg
     4801 gctagccaga aacggatctg gcgactggcg cagcgtctgg tggatatgcc gaatgtggtt
     4861 gaagccattc ccggcatgaa caatatcacg gtgattttgc gtaatcctga gtcgctggcg
     4921 ctggatgcca tagagcgttt gcaacgctgg tgggaggaga gcgaggccct ggaacctgag
     4981 tctcgcttta ttgaaattcc ggtggtttac ggtggtgcag gcggaccgga tttggcggtg
     5041 gtcgcggcgc attgcgggtt gagcgaaaaa caggttgttg aattgcactc ctccgtggaa
     5101 tacgtggtct ggtttttagg ttttcaaccg ggcttcccgt atctcgggag tttgccggaa
     5161 caactacaca cgccacggcg cgctgaaccg cgcttgctcg ttccggcagg ttctgtcggg
     5221 atcggcgggc cgcagactgg tgtttatccg ctggcaacgc cgggtggctg gcagttgatt
     5281 ggtcatacct cactcagcct gtttgatccg gcgcgtgacg aacccatctt attacgtccg
     5341 ggagacagcg tgcgctttgt accgcaaaag gagggagtat gctgaagatt attcgtgcgg
     5401 gcatgtatac cactgtgcaa gatggcggtc gtcacggttt tcgccagtcg ggtatcagcc
     5461 actgcggcgc actggatatg cctgcgttac gcattgctaa cctgctggtg ggtaatgatg
     5521 ccaatgcccc cgcgctggag atcacgctcg gtcagttaac ggttgagttc gaaactgatg
     5581 ggtggtttgc tctgacgggt gccggttgcg aagcgcggct ggatgataat gccgtctgga
     5641 ccggctggcg attgccgatg aaagcaggcc agcgtttaac gcttaaacgc ccgcagcacg
     5701 ggatgcgcag ttatctggcg gtcgcgggtg gtattgatgt tccgccggta atggggtcat
     5761 gcagcaccga tctcaaagtg gggattggcg ggctggaagg ccgtttactg aaggatggtg
     5821 accgactccc gattggcaaa gcgaagcgtg attttatgga agcgcagggc gttaaacagc
     5881 tgctgtgggg caaccgcatt cgcgccttgc cggggccgga atatcatgag ttcgatcgcg
     5941 cctcgcagga tgcattctgg cgttcgccct ggcagcttag ctcgcaaagt aaccgcatgg
     6001 gctatcgctt acaggggcaa attttaaaac gcaccaccga tcgcgaactg ttatctcacg
     6061 gtttgttacc gggcgtggtg caggtgccgc ataacgggca gcccattgtg ttgatgaacg
     6121 acgcacagac caccggtggt tacccgcgta ttgcctgtat cattgaggct gatatgtacc
     6181 atctggcgca aattccgctc ggtcagccga ttcattttgt ccagtgttca ctggaagagg
     6241 cactgaaatc gcggcaagat cagcaacgtt atttcgaaca attagcgtgg cggctgcaca
     6301 atgaaaattg acctgaacgc cgatctgggc gaaggctgca ccagcgacgc agagctatta
     6361 acgctggttt cctctgccaa tattgcctgt ggatttcatg caggcgatgc gcaaaccatg
     6421 caggcttgcg tgcgtgaagc aataaaaaat ggtgtcgcga ttggcgctca tccgagtttt
     6481 cccgacagga aaaattttgg tcgcagcgct atgcagctgc cgccagaaac cgtttacgcc
     6541 cagacgctgt atcaaattgg cgcgctggca acgattaccc gtgcgcaagg tggtgtaatg
     6601 cgtcatgtca aaccgcacgg catgttatac aaccaggcgg cgaaagaggc acaactggca
     6661 gacgccatcg ccagagcggt atacgcttgc gatccagcat tggttctcgt tgggctggca
     6721 ggaagcgagc tgattcgtgc aggcaagcaa tatggcctga caacgcgcga ggaagtgttt
     6781 gccgatcgcg gttatcaggc tgacggctcg ctggtgccgc gaagccagtc aggcgcgctg
     6841 attgaagacg aagaacaggc gcttgcgcaa acgttggaga tggtgcaaca cggcagagtc
     6901 aaaagtatca ccggcgaatg ggcaacggtc acggcgcaaa cggtctgcct gcatggcgac
     6961 ggcgagcacg cactggcgtt cgcccgccga ctacgctctg catttgccga aaaggggatt
     7021 gttgtcgcag cataaccccg attaataaag aatgaaaaaa ggatatcacc atgcctgaag
     7081 gcccggagat ccgccgtgca gcggataacc tggaggcggc gatcaaaggc aaaccactaa
     7141 ctgatgtctg gtttgccttc ccgcagttaa aaacttatca atcacaactt atcggtcaac
     7201 acgttaccca tgtggaaacg cgtggtaagg cgttgttaac tcatttttcc aacgacttaa
     7261 cgctctacag ccataatcag ctttatggcg tctggcgcgt ggttgatacc ggcgaagagt
     7321 cgcagaccac gcgagtattg cgggtaaaac tgcaaacggc tgacaaaacc attctgcttt
     7381 atagcgcctc ggatattgag atgttgaccc cggaacaact gaccacgcat ccgtttttac
     7441 aacgcgttgg tcccgatgtg ctggatccga atctgacgcc ggaggtggtg aaagaacggt
     7501 tattgtcgcc gcgctttcgt aaccgtcagt ttgctggatt actgctcgat caggcgtttc
     7561 tggctgggct tggcaattat ttgcgtgtgg agatcctctg gcaggttggg ttgactggaa
     7621 atcataaagc gaagacctca atgcggcgca actggatgca ctcgcacacg cgttactgga
     7681 tattcctcgg ttttcttacg ctacgcgggg gcaggtggat gagaataagc atcatggggc
     7741 gctgtttcgc tttaaggttt ttcatcgtga tggcgaactg tgcgaacgct gtggcggcat
     7801 cattgagaaa accacgctgt catctcgccc gttttactgg tgtccgggct gccagcacta
     7861 ggccgtacgt ttcggcgcat aggttgaaat aaaccgcgca atggcaggcc ctgtcagcaa
     7921 aatactgaac aggcgcaggg tttgcatcgc cataatgagc gccatatcgg cattgctccc
     7981 tgcggcgatg accgccacgg tatcaagccc gccggggctg gtggcgaggt aggcagtcat
     8041 aaaatcaatg tgcataaacc gggtcagtcc ccacgccata cccgcacaaa tagccagcag
     8101 agcaaaaatc gacagcagga tttgtggtag cgggcgtagt gcccgcagta agatttgttt
     8161 atcgaaacca aggccaatcc gccagccaat tgccatatac gccatcgcca gtagccattc
     8221 cggtagttcg atggtgatga gctgaccaga ctggagcact gcgcccgcca gcatcgggat
     8281 gagcatcgtg ccggaaggaa gacgcagcag gcatcccact gtaccggcaa cgaccgcgag
     8341 caaaatagta agcaggagat taatgctcac tggcgggaac cagacgatat gctggttaac
     8401 cgcttcagcg ttatcgccca gcatcattcg cgtgaccaga actgcggccc ccgcgacaaa
     8461 cagcactcgc agatactgca taaacgccac caggcggata tctgcgccgt aatcctgcgc
     8521 catcgcgacc attgccgccg ccccgccagg cgaggagccc caggcaccag tatttccggg
     8581 cagtgagcta tagcgcacca gtagccatcc cacgatagcg ctggaaagca acgtcaccag
     8641 taaaatagct aacacaatcg gccagttaac ctccagcgtg gtcaggatcg aaccggtcaa
     8701 gttttgcgca atcatgcagc caagaatggc ctgcgcggcg agaaaagcgg agcgggggag
     8761 ttgcagggta accccgcgca tactgaagat gatcccggcg atcatcggtc caagtaagag
     8821 tgccgccggg agatgtaatc cgaggaaacc aatagaaagg agaagtgata gcacacataa
     8881 cattccccac tgcaaaactg gcatcctttg ctccctcatt attttctctt tggtgtatcg
     8941 atacagcata aagaatgccg ggcatggaaa aaataacggt aatcaataaa agccggaaaa
     9001 ttggcaatta accgaagaag ccctctccgg ctggaaaggg caataggcta aatcagttgt
     9061 agttaatttt gaaaagtacc actgtatcaa agggaccaac gtcgatgggc gtacccggcg
     9121 tggatgacaa ttctgccgtg taggtttttt catacactaa caggtcgctg gtaaaatcgg
     9181 catactcact gtatttgttg aaagtatagg gtgaggcatt ttcatccaac aatcgaagct
     9241 tcaagccatt gccaatgagc agtgatttat cttcgtcgat aagggtttca tcggtataaa
     9301 acgaagagct gactttaaaa ccgtcggtac actggtcgtt ttgtgatttg gttgtagtta
     9361 ttttaaattg ctgcgacatc gtatggcgac gaatatccag cacgttaaat cggccaaaat
     9421 caacgacctg ggtttcaggt gagatagcaa agttgacgct gcaatccagc acgcggatat
     9481 tttccagacc ggtaatgtga tatttcagat tcttcgcggt agggtcttcg ttcacgctgc
     9541 ctgcgccatc aaactgtacg acgatgtaat cgctgagttt gctctgataa tcatggggcg
     9601 gcatggcgcg aactttaacg taaagccgca tacgcgccac aaacgtccgc gatttgtgca
     9661 taacggttgg atcgccggag caaagtttat tttgccagcc catcgcaatg atctgctccg
     9721 gggtgtaaat atcaatattc ttactgtcga tgcactgatt cgtgtcgatg cggcttttcc
     9781 caaggcttgc gtcatagtcc acgccttcat aggttacgcc caattgataa tagggatcct
     9841 gaataccggg gtaagggttt acccaggcgt agacgtgctc gctttcaaaa ttaccgtttg
     9901 tgttgttgtc acagtagacc ggaattttta tatcgtcaga ttcccagatt ttatcgccgg
     9961 gtttggaatt ggccggagcg gcgaagggca taatagcttc cgatttttct acagcgccgt
    10021 ttgcggtacc aaaatagcag ttcagcgcca gcgccgcttt tagcggcaaa aaaatcagac
    10081 agatggcgag caacacgcct ttggcgaatc tcatttttta ttctccaccg gctgcaggcg
    10141 gcattgccgc tggcaggaga gcgtgttcat acgcaatgcg ccgtagtcgt ccatgtagcc
    10201 aagataaaac tgcttgccgc tatagctgcc cgtgctggtg gtgaccgaac tgaacggtgc
    10261 gaccatcgtg cttttaaagc caggcagcac gcctttctca tcccgcccaa gataggcaat
    10321 agtcagatag tatccggtcg gggttttcag ggtaagctgg ttattctgct ggctaacctg
    10381 tagctgttgc tcaacatgat cgcccatttt tttgcgtaat gcggcgggtc gccagaatag
    10441 cttaatgcga ctttggacgg cgacttgcag gatcgcatgg ccctcgctgc cttccgggac
    10501 aggcggaatt tcacgcaggt tatagtaaaa gagagattcg cgatcggtgg gtaatttcgc
    10561 ggtggcggct tgtttcatga tccgaacctg cgaagtcgct tttggctcca gtcgctggat
    10621 aggcggcagg gcgacgagaa attcatcgct tttctctccc ttttcatttt ctatccatga
    10681 ataggcgagg taggggagtt tatcgctgcg gttttccacg cgcaggctgg ttgctttatc
    10741 tttgctatta aagatgatgc gcgtgcggtc aggctgcact gcgccgtggc tgcacatact
    10801 ggcaaccaga agaatcaaag ggaatccttt aacaaatgtc atcgtctttt ccttaattaa
    10861 tgacagggta aaatcaggcg cttagtcaca tcttccagat gttccggcag atgaatggca
    10921 cacgttttct ggtcgcccca gtgcacggta aattgttgat tttcatcgac cccactcagc
    10981 catgcgtgac cgttttcgcc aaccatcccg acgctgacac cgccttcagc ctggcgaaca
    11041 tcggcaccga gcggcgggaa atgcccgtta gcatcgctga taatgacgtt gagatctttc
    11101 ccggcgcggg acgccagcga tttaaagccg atggcgcctt cggtccaggt ttctttcacc
    11161 acgttttcgg aaacggtaac gccgtcaggc aagtcgttca tattgaccgc caccgtcgta
    11221 ggctggtagc tggaaacaaa cggtaccacg gcaataccaa agcggttggt gtagtcaata
    11281 ttgccctgaa tcggaatatc gcccacgccg tcggtgctga ccatcaggcg tggttcattg
    11341 gtggaactgc ggcgatggaa tgccgcgcca tgttgagtcg cggtaaacga gccgctccaa
    11401 ctggcactgg cggaagtgta atcattggcg gcataggttc cggtcagatc ccattccccc
    11461 gccgaactga ggtgttggta attgccgctg acctgcgcac cgttatccgg gcgatcggat
    11521 tgtattccag ccgacattcc ccaactgttt cgttcatcca gggtatcgtt ccacgacatg
    11581 cggtgggtgg tagtattact gttgttctgc atgtcataac ccagtcgacc actttcccca
    11641 atcggtaagg agatcgaaaa gtaaatctgg ttgtcgcgat ctttatcttc ataatgggtg
    11701 gtgttgaacg aggtcgagac agaaatatct ttccagtcgc caatgtcgac gttaaaaccg
    11761 acggtaatgt tggccgtggt ggaggtatcc gcattccacc agctctgatg caggatgttg
    11821 gcgtagagat taagattcag cagagtaata ggctgaccga aggagaggct gattgtttgc
    11881 ttttcatcct gcgcgtcggc gtcattgtat ttgtgatcaa tgtagttagc gtagctgtga
    11941 aaatggcgat cggagaagcg ataggctgcc aatgagatgg tgctgttggt cgcatcaacc
    12001 tgtttgctgt agttaaagcg gtagctgtaa ccctgctcat cctgttgggt atcaaaatgg
    12061 ctgtcggccc aggtcacgtc aaacgacagc gcccccatcc agagcatatt ttgcccaata
    12121 cccagcgcgc cggaacgata gtcatcgcct gcgagaagca tgccgccata tagtgaagtg
    12181 ttggagagca ttccccagga gacttcatgg ctaatgaagg tttcatcctc ggtgtggtgc
    12241 gacatagagg aacgcgggcg accggcagcc agtttatagc gcacctggcc ctgacgcgtc
    12301 aggaacgggg tagaggccgc tgaaacctgg aagttattca cccgtccatc ttcttcgctc
    12361 actttgacat ccagtgtacc ttgcactgac tgattcaggt catcaataat aaatgggcct
    12421 ggtggaactt ttttctggta aatcacgcga ccagaatggc ttatcgtgac cgtggcgtta
    12481 gtctgtgcga tgccgctgat ttgcggcgcg tagccgcgta actcccaggg gagcattcga
    12541 tcgtcgctgg ctaacgccgc accggtatag gagaaaccat caaaaatgtt agagctaaaa
    12601 tcggtttcgc ccagggttaa tcgggaacct atttgcggta acgggcggaa caggtaggtc
    12661 cgtgaaatcg ccccagattg ttcacggttg tcgccgctat cggactggct aagctgataa
    12721 tcgctgcgca gacgccatgc cccggcgttg aggcccgtgg tgccataggc gttcaggttg
    12781 ttgctgctgc tgccgctttg tgggcgataa gtgctggcaa acaggttgta atccatcagg
    12841 aagccaggaa taccgttatt ccacgttgag ggtggcgtcc agttttctga atgccacgcc
    12901 agccatgcct gcgggattgt aatattgagc tgttgatttg cctgatcgaa agtaaagatg
    12961 atttcaggga agacactaaa atcgacacat tcttttattg ccggtaattt tttacgaaag
    13021 tcagatttaa gtccgaagag ttcgatcagc gattcattga tacaaggaat aattttatcg
    13081 ccagattttt gccagcgaat ttgttgccca ttactgattt tattattatt aaccgtaaca
    13141 ctaacaaagt aatcgccggg agctacgacg cctttttgat tgagcaacga aatatcaacg
    13201 ctatcccgaa tcgacttatc gagaacgtta aggttaaatt cagctgtcag cgcaggaggg
    13261 gttaccattg ccagacagga aagaacagag agtcgataaa tattcacggc atccatacct
    13321 gaacaatatt cgcaaaaggc ggctaatcag ccgccttaaa aaggattatt ggtaatccag
    13381 aacgtaagtt gcgttagcag taacggcccc cgcggtaacc gaaactgcgt tatccatctg
    13441 ttccatccag gcaaagaagt ttaacgtttg ttctgttgaa cttgccaata agtcaattgt
    13501 tggcgcttct gttcccaatg taacaaatga ttcatctttg ttcatgagac gtacgccaac
    13561 gccggtagcc tcgccagcac tgatgttact aaggagcggg gttgcacctg tcgttttagc
    13621 cgtgctgtcg aaggtcacgc ctacctttga gaccacgcca cccttgccat catcccagcc
    13681 tgcaatatca cagttgatta agcgaatatt gacaggtttt ttctcgctat gatgttctcg
    13741 atttatgtgg gatgtggtta cttcacccag gtcaatgttc atatcaaggt catcagggtg
    13801 aatttcacat ggtgcttcaa taacttcacc tttaaagtgg atttcaccag atccttcatc
    13861 tgcagcaaaa acaggtgaag cgaatagtag agatatggcc agggcggcca gtgttttttg
    13921 tcctttaaac atacatgaat ccttcatggg ctatg
//
LOCUS       DBFIFI010000109        13834 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.109, whole genome shotgun sequence.
ACCESSION   DBFIFI010000109 DBFIFI010000000
VERSION     DBFIFI010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.109"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            138..1247
                     /gene="livK"
                     /locus_tag="N7B39_003054"
     CDS             138..1247
                     /gene="livK"
                     /locus_tag="N7B39_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HGU2422996.1"
                     /translation="MKRNAKTIIAGMIALAISHTALADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            1295..2221
                     /gene="livH"
                     /locus_tag="N7B39_003055"
     CDS             1295..2221
                     /gene="livH"
                     /locus_tag="N7B39_003055"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HGU2422997.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            2218..3495
                     /gene="livM"
                     /locus_tag="N7B39_003056"
     CDS             2218..3495
                     /gene="livM"
                     /locus_tag="N7B39_003056"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HGU2422998.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            3492..4259
                     /gene="livG"
                     /locus_tag="N7B39_003057"
     CDS             3492..4259
                     /gene="livG"
                     /locus_tag="N7B39_003057"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HGU2422999.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            4261..4974
                     /gene="livF"
                     /locus_tag="N7B39_003058"
     CDS             4261..4974
                     /gene="livF"
                     /locus_tag="N7B39_003058"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HGU2423000.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            5093..5839
                     /locus_tag="N7B39_003059"
     CDS             5093..5839
                     /locus_tag="N7B39_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HGU2423001.1"
                     /translation="MHYAVVVMLELLCCIHAYRTGQERYWIFIIFCFPVIGCVAYFVI
                     VMLPETGADRHGHTLLMRLQDKLNPERHLRKLTEELAIAETNQNHYALANELARLGRY
                     HEAVPHYQQALSGIFAHEAAMMLSLAQAQFAIQEFAACQQTLEDVMRYNPDFQSADGH
                     LLFARTLAAQEKYADAESEFEVLISYYPGPQARIYYAEMLAKMSRLREANEQYVAVVD
                     TAKRSRPHYRKHHREWIKTANERLKQSVVQ"
     gene            6199..7515
                     /gene="ugpB"
                     /locus_tag="N7B39_003060"
     CDS             6199..7515
                     /gene="ugpB"
                     /locus_tag="N7B39_003060"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HGU2423002.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            7613..8500
                     /gene="ugpA"
                     /locus_tag="N7B39_003061"
     CDS             7613..8500
                     /gene="ugpA"
                     /locus_tag="N7B39_003061"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HGU2423003.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSRFVGLDNFVALFHDSYYIDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVMQFRYVESKVRYQ"
     gene            8497..9342
                     /gene="ugpE"
                     /locus_tag="N7B39_003062"
     CDS             8497..9342
                     /gene="ugpE"
                     /locus_tag="N7B39_003062"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HGU2423004.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            9344..10414
                     /gene="ugpC"
                     /locus_tag="N7B39_003063"
     CDS             9344..10414
                     /gene="ugpC"
                     /locus_tag="N7B39_003063"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HGU2423005.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDICINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIALPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLPENQLHLFDGETGQRV"
     gene            10411..11154
                     /gene="ugpQ"
                     /locus_tag="N7B39_003064"
     CDS             10411..11154
                     /gene="ugpQ"
                     /locus_tag="N7B39_003064"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HGU2423006.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYGKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALSARELWAGMTPPLLSSFEIDALEAAQQAV
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(11141..11581)
                     /locus_tag="N7B39_003065"
     CDS             complement(11141..11581)
                     /locus_tag="N7B39_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HGU2423007.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPGTPQPDIPLKTIGP"
     gene            11701..13446
                     /gene="ggt"
                     /locus_tag="N7B39_003066"
     CDS             11701..13446
                     /gene="ggt"
                     /locus_tag="N7B39_003066"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HGU2423008.1"
                     /translation="MIKPTFLRRVAIAALFTGSCFSTVAAPPAPSPVSYGVEEDVFHP
                     VRAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLI
                     RSKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALD
                     KYGTMPLNKVVQPAFKLARDGFIVNDELADDLKTYGSEVLPNHENSKAIFWKEGEPLK
                     KGDKLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVER
                     TPISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYA
                     YADRSEYLGDPDFVKVPWQALTNKAYAKSIAEQIDINKAKPSSEIRPGKLAPYESNQT
                     THYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGG
                     DANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSLIITTVLQMVVNSIDYGMNVAE
                     ATNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELY
                     CASDPRSVDDLTAGY"
BASE COUNT         3091 a         3479 c         3939 g         3325 t
ORIGIN      
        1 aatcgctatt atcacaaaat actgcgctaa acccttaatc agacaggcaa aaacagtgca
       61 gtataaaaaa agaacagtct gatttgttaa cacataaaaa caaagcaaca caacatcacg
      121 aatggggatt tttgactatg aaacggaatg cgaaaactat catcgcaggg atgattgcac
      181 tggcaatttc acacaccgct ttagccgacg atattaaagt tgccgttgtc ggcgcgatgt
      241 ccggcccgat tgcccagtgg ggcgatatgg aatttaacgg cgcgcgtcag gcgattaaag
      301 acattaatgc caaaggggga attaaaggcg ataagctggt tggcgtagaa tatgacgacg
      361 cctgcgaccc gaaacaagcc gttgcggtcg ccaacaaaat cgttaatgac ggcattaaat
      421 acgttattgg tcatctgtgt tcttcttcta cccagcctgc gtcagatatc tatgaagacg
      481 aaggtattct tatgatctcg ccgggagcga ccaacccgga gctgacccaa cgcggttatc
      541 aacacattat gcgtactgcc gggctggact cttcccaggg gccaacggcg gcaaaataca
      601 ttcttgagac ggtgaagccc cagcgcatcg ccatcattca cgacaaacaa cagtatggcg
      661 aagggctggc gcgttcggtg caggacgggc tgaaagcggc taacgccaac gtcgtcttct
      721 tcgacggtat taccgccggg gagaaagatt tctccgcgct gatcgcccgc ctgaaaaaag
      781 aaaacatcga cttcgtttac tacggcggtt actacccgga aatggggcag atgctgcgcc
      841 aggcccgttc cgttggcctg aaaacccagt ttatggggcc ggaaggtgtg ggtaatgcgt
      901 cgttgtcgaa cattgccggt gatgccgccg aaggcatgtt ggtcactatg ccaaaacgct
      961 atgaccagga tccggcaaac cagggcatcg ttgatgcgct gaaagcagac aagaaagatc
     1021 cgtccgggcc ttatgtctgg atcacctacg cggcggtgca atctctggca actgcccttg
     1081 aacgtaccgg cagcgatgag ccgctggcgc tggtgaaaga tttaaaagct aacggtgcaa
     1141 acaccgtgat tgggccgctg aactgggatg aaaaaggcga tcttaaggga tttgattttg
     1201 gtgtcttcca gtggcacgcc gacggttcat ccacggcagc caagtgaaaa tcccaccgcc
     1261 cgtgaaatgc gggcgggttt agaaaggtta ccttatgtct gagcagtttt tgtatttctt
     1321 gcagcagatg tttaacggcg tcacgctggg cagtacttac gcgctgattg ccatcggcta
     1381 caccatggtt tacggcatta tcggcatgat caacttcgcc cacggcgagg tttacatgat
     1441 cggcagctac gtctcattta tgatcatcgc cgcactgatg atgatgggca ttgataccgg
     1501 ctggctgctg gtagcggcgg gattcgtcgg cgcaatcgtc attgccagcg cctacggctg
     1561 gagtatcgaa cgggtggctt accgcccggt gcgtaactct aagcgcctga ttgcactcat
     1621 ctcggcaatc ggtatgtcca tcttcctgca aaactacgtc agcctgactg aaggttcgcg
     1681 cgacgtggcg ctgcccagct tgtttaatgg tcagtgggta gtgggtcata gcgaaaactt
     1741 ctctgcctct attaccacca tgcaggcggt gatctggatt gtcaccttcc tcgccatgct
     1801 ggcgctgacg attttcattc gttattcccg tatggggcgc gcctgccgtg cctgcgccga
     1861 agatctgaaa atggcgagcc tgcttggcat taacaccgac cgggtgattg ctttgacctt
     1921 tgtgattggc gcggcgatgg cggcggtggc gggcgtactg ctcggtcagt tctacggcgt
     1981 cattaacccc tatatcggct ttatggccgg gatgaaagcc tttaccgctg cggtgctcgg
     2041 tgggattggc agcattccgg gagcgatgat tggcgggctg attctgggga ttgcggaggc
     2101 gctctcttct gcctacctga gtacggaata taaagatgtg gtctcattcg ccctgctgat
     2161 tctggtgctg ctggtgatgc cgaccggtat tctgggtcgc ccggaggtag agaaagtatg
     2221 aaaccgatgc atattgcaat ggcgttgctc tctgccgcga tgttttttgt cctggcgggc
     2281 gtctttatgg gcgtacaact ggagctggat ggcaccaaac tggtggtcga cacggcttca
     2341 gacatccgtt ggcagtgggt gtttatcggc acggcggtgg tctttttctt ccagcttttg
     2401 cgaccggctt tccagaaagg gttgaaaagc gtttccggac cgaagtttat tctgcccgcc
     2461 attgatggct ccacggtgaa gcaaaagctg tttctcgtgg cgctgctggt gcttgcggtg
     2521 gcgtggccgt ttatggtctc acgcgggacg gtggatatcg ccaccctgac catgatctac
     2581 attatcctcg gactcgggct gaacgtggtg gtagggcttt ctggtctgct ggtgctgggg
     2641 tacggcggct tttacgccat cggcgcttat acctttgcgc tgctcaatca ctattacggc
     2701 ttgggcttct ggacctgtct gccgatagcc gggttgatgg cggcggcggc gggcttcctg
     2761 ctcggcttcc cggtgcttcg cttgcgcggt gactatctgg cgatcgttac cctcggtttc
     2821 ggcgaaattg tgcgcatatt gctactcaat aacaccgaaa ttaccggcgg tccgaacggt
     2881 atcagtcaga tcccgaaacc gacttttttc ggccttgagt tcagccgtac cgctcgtgaa
     2941 ggcggctggg acacgttcag taatttcttt ggcctgaaat acgatccctc cgatcgtgtc
     3001 attttcctct acctggtggc gttgctgctg gtggtgctaa gcctgtttgt cattaaccgc
     3061 ctgctgcgga tgccgctggg tcgcgcctgg gaagcgttgc gtgaagatga aatcgcctgc
     3121 cgttcgctgg gcttaagccc gcgtcgtatc aagctgactg cctttaccat cagcgccgcg
     3181 tttgccggtt ttgccggaac gctgtttgct gcgcgtcagg gctttgtcag cccggaatcc
     3241 ttcacctttg ccgaatcggc gtttgtgctg gcgatagtgg tgctcggcgg tatgggctcg
     3301 caatttgcgg taattctggc ggcaattttg ctggtggtgt cgcgcgagtt gatgcgtgat
     3361 ttcaacgaat acagcatgtt aatgctcggt ggtttgatgg tgctgatgat gatctggcgt
     3421 ccgcagggct tgctgcccat gacgcgcccg caactgaagc tgaaaaacgg cgcagcgaaa
     3481 ggagagcagg catgagtcag ccattattat ctgttaacgg cctgatgatg cgcttcggcg
     3541 gcctgctggc tgtgaataac gtcaatcttg aattgtaccc gcaggagatc gtctcgttaa
     3601 tcggtcctaa cggtgccgga aaaaccacgg tctttaactg cctgaccgga ttctacaaac
     3661 ccaccggcgg cactatttta ctgcgcgatc agcatctgga gggtttgccg gggcagcaaa
     3721 tcgcccgcat gggcgtggtg cgtaccttcc agcatgtgcg tctgttccgc gaaatgacgg
     3781 taattgaaaa cctgctggtg gcgcagcatc agcaactgaa aaccgggctg ttctctggcc
     3841 tgttgaaaac gccttctttc cgtcgcgccc aaagtgaagc gctcgaccgc gccgccacct
     3901 ggcttgagcg cattggtttg ctggaacacg ccaaccgtca ggcgagtaac ctggcctacg
     3961 gtgaccagcg ccgtctggag attgcccgct gcatggtgac gcagccggag attttaatgc
     4021 tcgacgaacc tgcggcaggt cttaacccga aagaaacgaa agagctggat gagctgattg
     4081 ccgaactgcg caatcatcac aacaccacta tcttgttgat tgaacacgat atgaagctgg
     4141 tgatgggaat ttcggaccga atttacgtgg tcaatcaggg gacgccgctg gcaaacggta
     4201 cgccggagca aatccgtaat aacccggacg tgatccgtgc ctatttaggt gaggcataag
     4261 atggaaaaag tcatgttgtc ctttgacaaa gtcagcgccc actacggcaa aatccaggcg
     4321 ctgcatgagg tgagcctgca tatcaatcag ggcgagattg tcacgctgat tggcgcgaac
     4381 ggggcgggga aaaccacctt gctcggcacg ttatgcggcg atccgcgcgc caccagcggg
     4441 cgaattgtgt ttgatgataa agacattacc gactggcaga cggcgaaaat catgcgcgaa
     4501 gcggtagcga ttgtcccgga agggcgtcgc gtcttctcgc ggatgacggt ggaagagaac
     4561 ctggcgatgg gcggcttttt tgccgaacgt gatcagtttc aggagcgcat aaagtgggtg
     4621 tatgagctgt ttccacgtct tcatgagcgt cgtgttcagc gggcaggcac catgtccggc
     4681 ggtgaacagc agatgctggc gattggtcgc gcgctgatga gcaatccgcg tttgttgttg
     4741 cttgatgagc catcgctcgg tcttgcgccg attatcatcc agcaaatttt cgacaccatc
     4801 gagcagctgc gcgagcaggg gatgactatc ttcctcgtcg agcagaacgc caaccaggcg
     4861 ctgaagctcg ctgatcgcgg ctacgtgctg gaaaacggcc atgttgtgct ctccgatact
     4921 ggtgatgcgc tgctggcgaa cgaagcagtg agaagtgcgt atttaggcgg gtaaattatc
     4981 gataacccgg cagctaagtt ctgccgggtt attcgtccgt atacagactt gtttcaactg
     5041 accgggagga ttaaaaccag accacttatt tgccacggaa aggttccctc ccatgcatta
     5101 tgccgttgtt gtcatgcttg aactgctttg ctgtattcac gcttaccgta ccggacaaga
     5161 gcggtactgg atttttatca ttttctgttt tccggtgatt ggctgcgtgg cgtatttcgt
     5221 catagtgatg ctgcctgaaa cgggcgctga ccgtcacggg catactttgc tgatgcgcct
     5281 gcaggacaaa ctcaaccctg agcgccacct gcgcaaatta accgaagaac tggcgattgc
     5341 cgagacgaat caaaaccatt atgcgttggc taatgaactg gcgcgtctgg ggcgttatca
     5401 cgaagctgtt ccccattatc agcaggcgtt aagcgggata ttcgcccatg aagcggcaat
     5461 gatgctaagc ctggcgcagg cacagtttgc tattcaggag ttcgccgcct gtcagcagac
     5521 gctggaagat gtgatgcgtt ataaccctga ttttcagtcg gcagacgggc atttgttatt
     5581 tgccagaacg ttagccgcac aagaaaaata cgctgatgcg gaaagcgagt ttgaagtatt
     5641 gatttcttat taccccggcc cgcaggcgcg tatttactat gcggaaatgt tggcgaaaat
     5701 gagtcggtta cgtgaagcca acgagcagta tgttgccgtg gttgatacgg ctaaacgctc
     5761 aagacctcat tatagaaaac accaccggga atggattaag accgccaatg agcggctgaa
     5821 gcagagtgtg gtgcaatgaa aatcttcttt gccgttagac cagtgtcgcg gcaatctttt
     5881 gttaaaattg aaaaatgtag ctctcatcgg gcagcaaaat tggacgcaga atttgctgcc
     5941 atttttgctc ttcatggtaa ggagttctgg cgattagctg gccgttaatc gcctcttttc
     6001 agtgcattct cgggatatcc gtgccccatc tcccattttc cctcacccgt accgtcaccg
     6061 ccttgtcatc tttctgacac cttactatct tacaaatgta acaaaaaagt tatttttctg
     6121 taattcgagc atgtcatgtt accccgcgag cataaaacgc gtgaattcgc gcattcggta
     6181 caacaagaga gataaacgat gaaaccgtta cattatacag cttcagcact ggcgctcgga
     6241 ctggcgttaa tggggaatgc acaggcagtg acgaccattc cgttctggca ttctatggaa
     6301 ggggaactgg gtaaagaggt ggattctctg gcccaacgtt ttaacgccga aaacccggat
     6361 tacaaaattg taccgaccta taaaggcaat tacgaacaga atttaagcgc ggggattgcc
     6421 gcatttcgta ccggcaatgc tccggctatt ttgcaggttt atgaagttgg caccgccacc
     6481 atgatggcgt cgaaagccat taaaccggtt tatgacgtgt ttaaagaggc ggggattcaa
     6541 ttcgatgagt cgcagtttgt gccgacggtt tccggttact actccgacag caaaacgggc
     6601 cacttactct cccagccatt caacagctcg actcccgttc tctattacaa caaagacgcc
     6661 ttcaagaaag cggggttaga cccggaacaa ccgccgaaaa cctggcagga tctggcggac
     6721 tatgccgcaa aactaaaagc ctccggtatg aagtgcggct acgccagcgg ctggcagggc
     6781 tggatccaac tggaaaactt tagcgcctgg aacggtctgc cgtttgccag caaaaacaac
     6841 ggctttgacg gcacggatgc ggtgctggag ttcaacaagc cggagcaggt gaaacacatc
     6901 gccatgctcg aagagatgaa caagaagggc gatttcagct acgtcgggcg taaggatgaa
     6961 tccaccgaga agttctataa cggtgattgc gctatgacca ccgcctcttc cggttctctc
     7021 gctaatattc gcgagtacgc caaattcaac tatggcgtag gcatgatgcc ttacgacgcc
     7081 gatgcgaaag atgcaccgca aaacgccatt atcggcgggg ccagcctgtg ggtgatgcag
     7141 ggcaaagata aagaaacgta taccggcgtg gcgaagttcc tcgacttcct cgcgaagcca
     7201 gaaaacgctg ccgagtggca tcagaaaacc ggttatctgc caatcaccaa agcagcgtat
     7261 gacctgaccc gtgagcaggg cttttatgag aaaaacccag gggcggatac cgcgacgcgt
     7321 cagatgctga ataagccgcc gttgccgttc accaaagggc tgcgtctggg caacatgccg
     7381 cagatccgcg tgattgtgga tgaagagctg gagagcgtgt ggaccggtaa gaagacacca
     7441 cagcaggcac tggataccgc cgttgagcgt ggaaatcagt tgctgcgccg ctttgagaaa
     7501 tcgacgaagt cttaatcagt gtaatgtcgg atgcgtttcg cttatctgac ctggcatcgc
     7561 gtgtaggccg gataagcgaa gcgcatccgg cacagttcag gaattaaccg taatgtcatc
     7621 atcccgtccg gtgttccgct cgcgctggct gccttatctg ctggtcgcgc cgcagctcat
     7681 catcaccgtt atctttttta tctggcctgc gggcgaagcg ttgtggtact cgctgcaaag
     7741 cgtcgatccg tttggtttct ccagccggtt tgtcggcctg gataacttcg tcgcgctgtt
     7801 tcatgacagc tactatatcg actccttctg gacgacgata aaattcagca cctttgtcac
     7861 cgtcagcggt ttgctggtat cgctgttctt tgcggcgctg gtggagtaca tcgtgcgcgg
     7921 cagtcgtttc tatcaaacct tgatgctgct gccgtatgcc gtggctcccg ccgttgccgc
     7981 cgtattgtgg atcttcctgt ttaaccccgg tcgcgggctg atcacccatt ttctcgctga
     8041 gttcggctac gactggaacc acgcgcaaaa cagcggtcag gcaatgtttc tggtggtgtt
     8101 tgcctcagta tggaagcaaa tcagctacaa cttcctgttc ttctatgccg cgctgcaatc
     8161 cattccccgt tcgttgatcg aagccgcagc catcgacggt gcagggccga ttcgccgctt
     8221 ctttaagatt gcgctgccgc ttatcgcccc ggtgagtttc ttcctgctgg tagtgaacct
     8281 ggtgtatgcc ttcttcgaca ccttcccggt gatcgacgcc gccacgtccg gcgggccggt
     8341 tcaggccacc actacgctga tttataagat ctaccgcgaa ggctttaccg gactggatct
     8401 ggcttcgtct gccgcacagt cggtggtgtt gatgttcctc gtcatcgtgc tgacggtgat
     8461 gcagttccgc tatgttgaaa gcaaggtgcg ttaccaatga ttgagaaccg tccgtggctg
     8521 acgatattca gccataccat gctgatcctc gggatcgcgg tgatcctatt cccgctgtac
     8581 gtggcgtttg tcgcggcgac gctggataaa caggccgtct acgccgcgcc gatgacgctc
     8641 atccccggca cgcatctgct ggaaaacatc cacaacatct gggtaaacgg ggtaggcaca
     8701 aatagtgcgc cgttctggcg gatgttgctt aacagcttcg tgatggcgtt cagcattacg
     8761 ctcggcaaaa ttaccgtctc gatgctctcg gcattcgcca ttgtctggtt tcgttttccg
     8821 ctgcgtaacc tctttttctg gatgattttt atcaccctga tgttgccggt tgaagtacgt
     8881 atcttcccga cggtggaagt catcgccaac ctgaagatgc tcgacagcta cgcaggttta
     8941 acgctgccgc tgatggcctc ggcgaccgcg acgtttcttt tccgccagtt ctttatgacc
     9001 ctaccggatg aactggtgga agccgcgcgg atcgacggcg cgtcgccaat gcgcttcttt
     9061 tgcgacatcg tttttccgtt gtccaaaact aatctggcgg cgttgtttgt gatcaccttt
     9121 atttacggct ggaaccagta tttgtggccg ttgttgatta ttaccgacgt ggatctcggc
     9181 accaccgtgg cagggatcaa agggatgatc gccactggcg aaggcaccac agaatggaac
     9241 tcagtgatgg cggcgatgtt gttaaccctg atcccaccag tagtgattgt tttagtgatg
     9301 cagcgtgcct tcgtgcgcgg cctggtcgat agtgagaaat aagatggcag gactgaaatt
     9361 acaggcagta accaaaagct gggatggcaa aacccaggtg attaaaccgc tgacccttga
     9421 tgtggcggat ggcgaattta tcgtgatggt cgggccgtca ggctgcggga aatcgacgct
     9481 gctgcgcatg gttgccgggc tggagcgggt gacggaaggc gatatctgta tcaacgacca
     9541 gcgggtgacc gaaatggagc cgaaagatcg cgggattgcg atggtgttcc agaactacgc
     9601 gctttatccg catatgagtg ttgaagaaaa catggcgtgg gggctgaaaa ttcgcggcat
     9661 gggcaagcag caaattgccg agcgcgttaa agaggcggcg cgcattctgg aactggacgg
     9721 tctgcttaag cgccgcccgc gcgagctttc cggcggtcag cgtcagcgtg tggcgatggg
     9781 ccgggcgatt gtgcgcgatc cggcggtgtt cctgtttgat gagccactct ctaacctcga
     9841 tgccaagctg cgcgtacaga tgcgtcttga actgcaacag ctgcaccgtc gcctgaaaac
     9901 gacttcactc tacgttactc acgatcaggt tgaggcgatg accctcgccc agcgagtaat
     9961 ggtgatgaac ggcggcgttg ccgaacagat tggcacacca gttgaagtct acgaaaagcc
    10021 cgccagcctg tttgtggcga gttttattgg cagcccggcg atgaatctgc tgacaggccg
    10081 cgtgaataac gaaggcacgc acttcgagct ggacggcggc attgcgctgc cgctaaacgg
    10141 tggctaccgt cagtatgcag ggcgtaaaat gactctcggc attcgcccgg aacatatcgc
    10201 gctaagctcg caggcagaag gcggcgtgcc gctggtgatg gacacgctgg agatcctcgg
    10261 cgcagataac ctggcgcacg gacgctgggg cgaacagaag ctggtggtac ggctggcgca
    10321 tcaggagcgc ccgacggcag gcagcacgct gtggctgcat ctgccggaaa atcagctaca
    10381 tctttttgat ggtgaaacag gacaacgagt atgagtaact ggccttatcc ccgcatcgtc
    10441 gcccaccgtg gcggcggtaa actggccccg gaaaacaccc tggcggcaat cgacgtcggg
    10501 gcaaaatacg gtcataagat gatcgaattt gacgcgaagt tgtcgaaaga tggcgaaatc
    10561 ttcctgctcc atgacgacaa tctcgaacgt accagcaacg gctggggcgt cgcgggtgaa
    10621 ctgaactggc aggatttact acgtgtggat gcaggcagtt ggtacggcaa agcgtttaaa
    10681 ggtgagccgc tgccgttgct ttcgcaggtg gcggaacgct gtcgcgaaca tgggatgatg
    10741 gcgaatatcg aaatcaaacc caccactggc actgggccat taacgggcaa aatggtggcg
    10801 ctgtcggcac gcgagctgtg ggccggtatg acgccgccgc tactgtcatc gtttgagatt
    10861 gatgctttag aagccgcaca acaggcagta ccagaactgc cgcgcggttt gttgctggat
    10921 gagtggcgcg acgactggcg agaactgacc gcgcggctgg gctgcgtctc tattcatctc
    10981 aatcataagt tgctcgataa agcgcgagtg atgcagttga aagacgccgg attacggatt
    11041 ctggtttata ccgtcaataa accccagcgc gcggcagagt tgctgcgctg gggcgtggat
    11101 tgcatctgta ccgatgcgat tgatgtgatt ggtccgaact ttacggccca atagttttca
    11161 acggtatatc aggctgcggc gtgccgggct gattcagcat gtcgccgttt ctcctctccg
    11221 gcaacatatg ctgctgattc aacgaactat ccggattacg gttgctgttt aacatcccgc
    11281 cattggtgtt gggcagcatt tgctgccggg cgggatttcg ctcccccggc tgtgactgca
    11341 acacccgctg agaattattg tttatctggt tttctaaatg ctgttgttgc aactgagttt
    11401 gcgttttcag ttgctgattc agcatccctt tttgctggat ttgctgcgtt tgcatctggg
    11461 tttgcatccg ctgctgactg gggatctgat accccggctg gttagggttg ttcagagtat
    11521 taatgggctg tgcaaagccg acaaacggca ggagtgccgt aagaatcaga agtcgtttca
    11581 tcgcgtatcc tcctctgaag atattcttta agtttactcc tttcctgcaa tcatcaggat
    11641 taattcagag ttatatacca ggcttagctg gggttgcccc ttaatctctg gagaataacg
    11701 atgataaaac cgacgttttt acgccgggtg gccattgctg ctctgttcac aggaagttgt
    11761 tttagtacgg tagccgcgcc acctgcgccg tctcctgttt cttacggcgt ggaggaagat
    11821 gtctttcatc cggtacgcgc gaaacagggg atggtggcat ctgtagatgc tactgccact
    11881 caggtggggg tggatatcct caaggagggc gggaatgccg ttgatgccgc cgtggctgtg
    11941 ggctacgcgc tggcggtaac gcatccgcag gcagggaatc tgggcggtgg tggttttatg
    12001 ctgatccgct cgaaaaatgg caataccacc gctatcgatt tccgcgaaat ggcacccgcc
    12061 aaagcgaccc gcgatatgtt ccttgatgac cagggcaacc cggacagcaa aaaatcactc
    12121 acttcgcatc tggcttccgg cacgcccggt acggtcgctg gtttctcgct ggcgttggat
    12181 aaatacggca caatgccgtt gaacaaagtg gtgcagccag cgtttaaact ggcacgcgat
    12241 ggttttatcg ttaacgacga gctggctgac gatctcaaaa cctacgggag cgaagtgctg
    12301 ccgaatcacg aaaacagcaa ggctatcttc tggaaagagg gcgagccgct gaaaaagggc
    12361 gacaagctgg tgcaggcgaa cctggcaaag agcctggaga tgattgccga aaacggcccg
    12421 gacgaattct acaaaggcac gatagcggaa cagattgccc aggagatgca gaaaaacggt
    12481 ggcctgatca ctaaagaaga tttagcggcg tataaagcgg tcgaacgcac accgataagc
    12541 ggcgattatc gcgggtatca ggtttactcc atgccaccgc catcctccgg cgggatccat
    12601 atcgtacaaa tcctcaatat tctggaaaac ttcgatatga agaaatacgg ttttggcagc
    12661 gccgacgcga tgcagatcat ggcagaagcg gagaaatatg cctacgctga ccgctcggaa
    12721 tatcttggcg acccggattt tgtgaaagtc ccgtggcagg cgctgaccaa taaagcctat
    12781 gccaaatcta ttgccgagca aatcgatatc aataaagcga aaccgtccag cgagattcgc
    12841 cccggcaagc ttgcgcctta tgagagtaat caaactaccc attattcggt ggtggataaa
    12901 gacggtaacg cggtggcggt gacctatacg ctgaacacca ccttcggtac gggtattgtc
    12961 gcgggcgaga gcggtatttt gctcaataac caaatggatg atttctctgc caaaccgggc
    13021 gtaccgaacg tttacgggct ggtgggcggt gatgccaacg ccgtcgggcc gaacaaacgc
    13081 ccgctgtcat cgatgtcacc aaccattgtg gtgaaagacg gtaaaacctg gctggtcacc
    13141 ggtagccctg gcgggagcct gatcatcact acagtgcttc aaatggtggt gaatagcatc
    13201 gattatggca tgaacgtcgc cgaagcgacc aatgcgccgc gtttccacca tcagtggttg
    13261 ccggacgagc tgcgtgtcga aaaagggttt agcccggata cgctcaagct gctggaagca
    13321 aaaggtcaga aagtggcgct gaaagaggcg atgggcagta cacaaagcat tatggttggg
    13381 ccggacggtg agttgtactg cgcatccgac ccgcgctcgg tggatgattt aacggcgggg
    13441 tactaaggtt agcggccctc ttcgtgggaa gagggctgtt ttcttgaaca agataaatta
    13501 gtgtctggat tgcttttact attgctccgt aatatataat cataattatc ctgaatgctt
    13561 ttaaggggct taggttatgc tttcttttca agagtatgca gaatagtctt tcatttaccg
    13621 tgaaaatagg acgtgcgata attacttcct gatattcagg aaaggaatta actatgctat
    13681 taaataaact gtagttatag ttcattttag agtgtaattt attgatttaa atatcacgct
    13741 ttgtgttgtt tatgtgcata atggcattca tcatgacttt gatcgatttt gtaaagtgtt
    13801 tttaaggggg aataatgaaa gcaaaatacg tgat
//
LOCUS       DBFIFI010000110        13434 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.110, whole genome shotgun sequence.
ACCESSION   DBFIFI010000110 DBFIFI010000000
VERSION     DBFIFI010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13434
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.110"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(145..297)
                     /locus_tag="N7B39_003067"
     CDS             complement(145..297)
                     /locus_tag="N7B39_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HGU2423009.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(561..1295)
                     /gene="cysH"
                     /locus_tag="N7B39_003068"
     CDS             complement(561..1295)
                     /gene="cysH"
                     /locus_tag="N7B39_003068"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HGU2423010.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAQLEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKNGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            complement(1369..3081)
                     /gene="cysI"
                     /locus_tag="N7B39_003069"
     CDS             complement(1369..3081)
                     /gene="cysI"
                     /locus_tag="N7B39_003069"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HGU2423011.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(3081..4880)
                     /gene="cysJ"
                     /locus_tag="N7B39_003070"
     CDS             complement(3081..4880)
                     /gene="cysJ"
                     /locus_tag="N7B39_003070"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HGU2423012.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLTAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGVVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQEEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5199..5561
                     /gene="queD"
                     /locus_tag="N7B39_003071"
     CDS             5199..5561
                     /gene="queD"
                     /locus_tag="N7B39_003071"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HGU2423013.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5639..6910
                     /gene="ygcN"
                     /locus_tag="N7B39_003072"
     CDS             5639..6910
                     /gene="ygcN"
                     /locus_tag="N7B39_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HGU2423014.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDCATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLARFKTHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDTALTGAQAAAQTLISACQHRE
                     PQNLFPLYHHNVERSLLWDVLQRYQHVPVLLQRPGWYRTWPALMQDISRDLWDQGDKP
                     VPPLRQLLWRHLRRHGLWNLAGDVIRSVRCL"
     gene            6901..7161
                     /gene="ygcO"
                     /locus_tag="N7B39_003073"
     CDS             6901..7161
                     /gene="ygcO"
                     /locus_tag="N7B39_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HGU2423015.1"
                     /translation="MSVARNLWRAADAPHIVPADSVERQMVQRLINACPVGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7178..7753
                     /gene="ygcP"
                     /locus_tag="N7B39_003074"
     CDS             7178..7753
                     /gene="ygcP"
                     /locus_tag="N7B39_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HGU2423016.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     QIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7901..8761)
                     /gene="ygcQ"
                     /locus_tag="N7B39_003075"
                     /pseudo
     CDS             complement(7901..8761)
                     /gene="ygcQ"
                     /locus_tag="N7B39_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
     gene            complement(8758..9537)
                     /gene="ygcR"
                     /locus_tag="N7B39_003076"
     CDS             complement(8758..9537)
                     /gene="ygcR"
                     /locus_tag="N7B39_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HGU2423017.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAHGNSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFASE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDAPFI
                     TLEQRTEHGLRCCRIRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAELPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(9515..10851)
                     /gene="ygcS"
                     /locus_tag="N7B39_003077"
                     /pseudo
     CDS             complement(9515..10851)
                     /gene="ygcS"
                     /locus_tag="N7B39_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417251.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(10945..12399)
                     /gene="ygcU"
                     /locus_tag="N7B39_003078"
     CDS             complement(10945..12399)
                     /gene="ygcU"
                     /locus_tag="N7B39_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HGU2423018.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVNCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(12469..13254)
                     /gene="ygcW"
                     /locus_tag="N7B39_003079"
     CDS             complement(12469..13254)
                     /gene="ygcW"
                     /locus_tag="N7B39_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU2423019.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     VFIPSFVKDNGETKEMIENQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASQASNYVNGHLLVVDGGYLVR"
BASE COUNT         3155 a         3652 c         3367 g         3260 t
ORIGIN      
        1 tatgaataaa ttaatgattt tcatttgaaa ttcataatga taaatagaga ctatatattc
       61 acataaaaaa tataaattta aaaaagggta caggagacca aaccatcaca attcaatacg
      121 cggggtttta cccccgcgtg gtggctactt cttcgattcg taagcgagaa ctgccttaaa
      181 ctccatacca cgctctctga tactcaactc acataacgag tcgcctacca tgagcgtgaa
      241 tcccagtact gtacaacaca gtactatgat tgccacaagg gcatattttg ttagcatgtc
      301 ttgcctccct gcaaagaaga gactaacatc cgaattgcta cgttcgaacg cgagcctcag
      361 gttgatagaa atatcgcctg gggcttttgt ccatctggaa cctcgcgaat gcttaacgcc
      421 agacagcctc aagcacccga cgccattcta tacctgataa ttcttccagc ggcgttcacg
      481 tttaaaatca ggaatgctgc tcgcaaaata aaaacgcccc catcattact gacagaggcg
      541 tttaatttat ccggcaatat ttacccttcg tgtaacccac attccctttt taagccaaag
      601 aaacgcgttt cttcttccgc catgccgggt tcccatttac gggttgtatg ggtatcgccc
      661 accgataaat atccttcgtc ccataatggg tgatatttca ggccattctt ttgcagatac
      721 tgatatatcg ttcggttatc ccagtcgata atcggcagca ctttaaatac gccacgctga
      781 attgccagca ccggtaaatt ggcgcgactg ccggattgtt cacggcgcag gccagcaaac
      841 caggtttgcg cgttaagttc tttcagcgcc cggttcatcg gttcgacttt gttgatgtca
      901 ttgtactttt caatgccttc aacgccctgt tcccacagtt ttccgtagcg tgcttcctgc
      961 caggctgcgc tttcggtagc acggtacact ttcaggttga gcttgagttt gtccgttaac
     1021 tcgtcaataa agcggtaggt ttccgggaac aagtaacccg tatcggtgag gatcaccgga
     1081 atatccgggc gaatttgatt caccagatgc aggctcaccg ccgcctgaat gccaaagctg
     1141 gaagaaagta catattcacc gggcagatta tccagcgccc aggctacgcg gccttcagcg
     1201 tccagttttt ccagttgggc gttagtttcc gccagcgcca gaatgcgatc taccttcggc
     1261 agttcgttca gggcgtttag atcgagtttg gacatagatt cctcactgtt tgccttgcct
     1321 gatgcgacgc tcacgcgtct tatcaggcct acaagaccgg gctgatggtt aatcccacaa
     1381 atcgcgcgcc ggatcgagca ccgggcgaat gatgcccgca cgcaccgtaa agtcgccgaa
     1441 gccttcaccc gcttcgcgct ctttcgccca gcgccctatc agttcatcaa gcgacgccag
     1501 gatttccggc tcggtgatgt tttctttata catccgtggg atacgtgtcc caatgcggtt
     1561 gccgccaaga tgcaggttgt agcgacccgg cgctttaccc accaggccca cttccgccag
     1621 catcgcgcga ccacaaccgt tcgggcagcc tgttacacgc atcacgatat gctcatcgct
     1681 gacaccatgt ttcgccatta aattatcgat gttgtcgata aaagacggca ggaaacgctc
     1741 cgcttccgcc atcgccagcg ggcaagtcgg gaacgagacg caggccattg agttttcacg
     1801 ctgcggcgtg acggcattca ttaacccgct ctctttggcg atcttctcga tcttcgcttt
     1861 ctcgctttcc ggcacaccgg cgatgatcag attctggttc gccgtaatgc ggaaatcacc
     1921 tttgtggatc ttcgcgatct ccagcaggcc ggttttcagc ggacgccccg gataatcaag
     1981 gatgcgacca ttttcgataa acagcgtcag gtgccagtta tcatcaatgc ccttaaccca
     2041 gccaatacga tctccgcgtc cggtgaactc atacggacgg atcggttcaa atttgatccc
     2101 cgcgcgacgc tccacttccg ctttaaacgt ctcaaccccc acgcgctcca gcgtgtattt
     2161 ggttttggca tttttacgat cggttcggtt accccagtca cgctgagttg tcacgacggc
     2221 ttccgccact gccagcgtat gctccagcgg cagatagcca aactcactcg ccgtgcgggc
     2281 gtaggttttc ttgttgccgt gttcgataga aagtccaccg cccaccaaca ggttaaagcc
     2341 caccagcttg ccgttttcgg cgattgccac gaagttcatg tcgttggcgt gcagatcgat
     2401 atcgttctgt ggcgggatca ctaccgtggt tttgaattta cgcggcaggt aggtctggcc
     2461 gaggatcggt tcttcatcgg tggtggcgac tttttcctga tcgagccaga tctccgcata
     2521 cgcgcgggta cgaggcagca gatgttcaga gattttcttc gcccactcgt acgcttctgc
     2581 gtgcagctgc gactcgtaag ggttcgaggt gcagagtacg ttacggttca tgtcgttagc
     2641 ggtcgccagc gcatcaagac cgaccgagtg cagcatctgg tgcaccggtt tgacgttctt
     2701 tttcagaatg ccgtggaact ggaatgtctg gcggttggtc aggcgaatgc tgccatagat
     2761 ggtgttttca ccggcaaatt tatcgatcgc ctgccactgt ttggtggtga taactccacc
     2821 cggcagacgg cagcgcaaca gcatcgcatg gcgcggctcc agcttctgtt cagcacgttc
     2881 ggcgcggatg tcgcggtcat cctgctgata catgccgtgg aagcggatca gcaggaagtt
     2941 atcgccctta aagccgccgg tcaggccgtc gtttaaatct tccgcaatgg tgccgcgcag
     3001 gtagttgctt tcaagcttca tgcgctcggc gtctgtcagt tttccttcga ccactaaagg
     3061 ccctggatgt ttttcgctca ttagtagaca tctcgctgat aacggcgctc tacgcgcagc
     3121 tcacttaaaa attcatccgc cgcttcggtg tccatgccac caaattcggc aatcacttcc
     3181 agaagtgcct gctcaacgtc tttcgccatg cgattagcgt cgccgcagac ataaatgtgg
     3241 gcaccgtcat tgatccagcg ccacagctcc gcgccctgtt cgcgcagttt gtcttgtacg
     3301 taaacttttt ctttttgatc gcgcgaccag gcaagatcga tacgcgtcag cacgccctct
     3361 ttgacgtaac gctgccactc cacctggtag aggaaatctt ccgtaaagtg cgggttgcca
     3421 aagaacagcc agtttttacc cggcgcttcg tcagcggcgc gctgctgcat aaaggcgcgg
     3481 aacggcgcga tgccggtgcc tgggccaatc ataatcaccg gggtttccgg gttagcgggc
     3541 aggcggaagt tatcgttatg ttcgataaat acgcgaactt cgccctcttc ttccacgcgg
     3601 tccgcgagga agctggaggc accaccggca cgggcgcggc cttccacgtc gtaacgcacc
     3661 acaccaacgg tgacgtgtac ttcgttctcg acttcctcct gcgaggaggc gatggaatac
     3721 agacgcggcg tcagcgggcg cagcagatta atcagcgctt cggcgtccag ttgcgccgga
     3781 gagaagcgca ccatgtcgac aatcggcgta gtcgcggcgt aatgctgtaa cttcgcttta
     3841 tcgcccacca gcggcaacag cgtttcgctg cgggtaagcg tggcatagtt ctcaacaata
     3901 ttggcggtgt tgacggtcag ttcgaagtgc cactgtagcg cttcgttcag aggcaacgtt
     3961 ttgccctcga cggtgacagg ttcatcgcct ttcagccaca gcagttcgac aagttctttc
     4021 accagtgccg gatcgttctg ataccagaca cccagcgcgt cacccggctg gtaacgcagg
     4081 cccgagtcac ctaagtcaat ttcgatatgg cgaacgtctt tttcagagtt acgcccggta
     4141 attttctggt taaccgaaag gctcgctacc agcggcgcgt ctttgctgta cgggctggtg
     4201 tggatttcat ttaccacgcc agtagcgacg gattgcgaag gtgccgcgac aggcgcacgc
     4261 gatttaagcg catcaaccac gcgggcgcgc cactcgctgg cagcagcctg gtattcaacg
     4321 tcggcgtcga cacggtcgag caggcgttca ccgcccagtt ccgccagctt gctgtcgaaa
     4381 tctttcccgg actggcagaa aaattcataa gaactatcgc cgaggctaaa cacggcaaaa
     4441 gcggtatttt ccagcttcgg cgcttttttg gagaacagga acttatgcag cgcgacggct
     4501 tcttccggcg gttccccttc tccttgcgtt gacgtcacca cgataagcag tttttcgctg
     4561 gcgatttgtt tgaatttata gtcgcccgcg ttcaccagct taacgttcag ttttgccgtc
     4621 aataaatcat cgcgtaatgc ttcagcaacc cggcgcgcat tgccggtttg cgaagcagag
     4681 ataatagtta tacccggcat ttctgcggct ggcgctggcg tcgctgcaag cgcagcaggc
     4741 tgctgattga gcacgcccca gaaatagcca gaaacccagg caagctgggt gggagttaaa
     4801 tcggtcgtgg ccgcctgaag gcgtgccagt tgctccgggt tcaacggaag caacgcggaa
     4861 ggtgggacct gtgtcgtcat gcgtcgttat gttccagtaa gcaaagctgt ttctgcgccc
     4921 tgtcagcgcc cataaaacag aagagaaggt aaggttaacg gggtgaacgg tgtggattaa
     4981 agacgggata gcgataacta ataaccaaat cgactaacct gttttagcaa tagtctttaa
     5041 caacaaaata gattaaccaa cctaatgaaa aacaaatgaa tttagccaat cattaagata
     5101 aatcagcgat tttgcgcaac aaagtcgttt tagataatgc gaaaaaagag cctttccggt
     5161 actctacggc ggttttatcg tcctgtagag aaattatgat gtccaccacg ttatttaaag
     5221 atttcacctt cgaagccgct caccgcttac cacacgtccc ggaagggcat aaatgtggtc
     5281 gcctgcacgg gcattccttt atggtgcgac tggaaattac cggggaagtc gatccgcata
     5341 cgggctggat tatcgatttc gctgaactaa aagcggcgtt taagccaact tatgagcgcc
     5401 tcgatcacca ttatctcaat gatattccag gtctggaaaa cccaactagc gaagttttag
     5461 caaaatggat ttgggatcag gttaaacccg ttgtgccgct gttaagtgcg gtgatggtaa
     5521 aagaaacctg caccgctggt tgtatctatc gcggcgaatg ataagagtgt ggcggcggtc
     5581 aatttccctt aagtaacgct atgttagggt gttgtgttca ggatatctgg ggcatgacat
     5641 ggaagacgac tgcgacatta ttattattgg tgccggtatt gcaggcaccg cttgcgcgtt
     5701 acgctgcgcg cgagcgggtt tatccgtttt gttactggaa cgcgctgaaa tccccggcag
     5761 caaaaatctt tccggcgggc ggttatatac ccatgcactc gcggaactcc tccctcaatt
     5821 tcatctgacc gcgcctcttg aacgacgcat cactcacgaa agcctttccc tgttaacgcc
     5881 ggattgcgca acgacgtttt ccagcttaca gcccggcggt gaatcctgga gtgtattacg
     5941 tgcacggttc gatccgtggc tggttgccga agccgaaaaa gaaggtgtcg aatgcatccc
     6001 tggtgcgacg gtggatgcac tgtatgaaga aaacggcagg gtgtgtggtg tcatttgtgg
     6061 tgacgatatt ctccgcgccc gttatgtggt gctggcagaa ggtgccaaca gcgtcctggc
     6121 tgaacgtcac gggttagtga ctcgtcctgc tggcgaagcg atggcgttgg ggatcaaaga
     6181 agtgctgtcg ctggaaacat ccgctattga agaacgtttt catctggaga ataacgaagg
     6241 cgcagcgttg ctgttcagcg gcggaatctg tgatgactta cccggcggcg catttcttta
     6301 tactaatcaa caaacgctct cgttagggat tgtttgccca ctctcttccc ttacgcaaag
     6361 tcgtgttccg gcaagcgagc tgcttgctcg ctttaaaacg catccggcag tgcgcccgct
     6421 tatcaaaaac acggaatcac tggagtatgg tgcgcatctg gtgccagaag gtggcttgca
     6481 cagtatgccg gtacaatacg ccggtaacgg ctggctgctg gtgggcgatg cgttgcgcag
     6541 ttgcgtcaat accggaattt ctgtgcgcgg catggatacg gcgctaactg gcgcgcaggc
     6601 ggcggcacaa acactgataa gcgcctgcca gcaccgcgag ccgcaaaatc tgtttccgct
     6661 ttatcatcac aacgtagagc gcagcctgct gtgggatgtt ctacagcgtt atcagcatgt
     6721 tccggtgctt ttgcaacgcc cgggatggta ccgtacgtgg cctgcgttaa tgcaggatat
     6781 ttcccgcgat ttatgggatc agggtgataa acctgttcca ccgctgcgcc agttactctg
     6841 gcgtcattta cgtcgtcatg gcttgtggaa tctggcgggc gatgttatca ggagtgttcg
     6901 atgtctgtag cccgtaatct ctggcgcgct gctgatgcgc cgcacattgt tccggctgac
     6961 tccgttgagc gtcagatggt acaacggttg attaacgcct gtccggtagg tcttttttcg
     7021 ctcacaccgg aaggtgactt acgcgttgat tatcacggct gcctggagtg tggcacctgc
     7081 cgtttgctgt gcgacgaatc cacactacaa cagtggcgct atccgccttc cggattcggc
     7141 atcacctacc gatttggata aataataagg ataatttatg cccctcttac acctgctccg
     7201 ccagaatccg gtgattgctg ccgttaaaga taatgccagc ctgcaactgg ccatcgattc
     7261 tgaatgccag tttatttccg tcttgtacgg caatatctgc accattagtc aaatcgttaa
     7321 aaagattaag aacgctggga aatatgcttt tattcatgtt gatttgctgg aaggcgcgtc
     7381 aaataaagaa gtggtgattc agtttttgaa actggtgacc gaggcggatg gaattatcag
     7441 caccaaagca tcgatgctaa aagcggcaag agcggaaggt tttttctgta ttcatcgcct
     7501 gtttattgtt gattcgattt cgtttcacaa cattgataag caagttgcgc aatcgaatcc
     7561 ggattgtatt gagatcctgc caggctgcat gcccaaagtg ctgggctggg tgacggaaaa
     7621 aatccgccaa ccgctgattg ctggcgggct ggtatgcgat gaagaagatg cgcgtaatgc
     7681 gattaacgcg ggtgtcgtgg cgctttccac cacgaatacc ggagtctgga cgttagcgaa
     7741 aaaattgctt tgacgggata atcgtaacca attgaatttg gtttgatttg taggccgcac
     7801 gccacatccg acattcagcg cctgatgcga cgcttaacac gtcttatcag gcctacaggt
     7861 ccccagtcca gaaccgtagg caggatcatc cggcagcacg ttattggcat tctgcgtcaa
     7921 tgtttgtcac cagtgcctcc agcatcactt tccagtcatc aaccacgcca acatctgcct
     7981 gtgaaaatac cgctgcgctg gcgtcatgat taatcgccac cacaaacttg ctattgcgta
     8041 cgcccgccat taacgctgct gcgccagaag cgccaacaac aatacacacc tcaggtgcca
     8101 gcagatgtcc ggaaatacca atcacttttt cagcatcaac gccaccgttc atcactcgcg
     8161 cccgactgta gcccacttcg gctcccagct tttatgccag catagcgatt tcctggttat
     8221 ctgcctctcc gccctgcccc accaccaaca cccgtctggc ctcagctaat gggtcgcggg
     8281 taacattttt caggttctct atgctaacca gccagtccgg aagcgcacct gggactatgt
     8341 tcagttgctg cataccagag ggcaacgtgg catttttatc tgctcccgcc tggcgcgcca
     8401 gggaaagaca tagcgggcgt ttttctgttt gcagcgtcgc cgttagcgca tttccccagt
     8461 gggatttgcg cacgcttacg attggaatct ctaacgaagt cacctggcag atgcttgcac
     8521 catgtaaacg ccaggccagc cgagtcgaca gttcatcgcc aaacgttccc ggcggaaaga
     8581 gaacaacgtt cgcaggcgtt cgctgccact gttccaccag cgcatccagc acctgttcca
     8641 ccactacggg ctgtggttct atttgccagt gcgccagggt acaaccgctg aaatcctgcg
     8701 ccgccagcca gcccgctaat gcggcattct cctgatttat tgtcacaatt gcgatattca
     8761 tggctgcatc ctttggcgta aatagtcctg ccatagcttt tgcgcttttt ctgctaccgt
     8821 ttgcccatca atcagcgtcg ctccgcgtcg ttgctctgct cttgccagtt gcagacactg
     8881 catcgctggc aattcggcag caacagtttt acggataatt tccgctttcc ccgcagccat
     8941 tcgctggcgc attcccggta ctggcaaggc cacttcaccg cactgacgca cggcaatcac
     9001 cgcaggcaag cgaatccgac aacagcgcag cccatgttca gtacgctgtt cgagggtgat
     9061 aaacggcgcg tcgagagtga aacgctccac ctgggtgaag caaggccagc ccagcatttc
     9121 cgccagcaaa aatggcgttt gcccattctg cccttcgctg ctttggcagc cggtaattat
     9181 cagatccagc ggattctgac gctgccattc ggcaatatgg cgagcgacaa attccgacgc
     9241 aaagcgcaga tctgccgccg tctccagcaa tacagcttcc tcaaacccaa gagccatgag
     9301 atagcgcagc cagtgcaacg cccgttcatc ccccatgctc aacgcggtta aagacatcgg
     9361 cgtaccgttt tttctctgcg ccaacagcag cgcggcggca gcctgttcat cagcaccgag
     9421 taaacttcgc agtagcgaaa tatccggtcc gctattacca tgagccgccg cctgccactc
     9481 tttttccgcc agcattccgg catccggttc ggctttaaac gctaacagaa tgttcattcg
     9541 cacctcctac atttcccgcc gccaccagcg ggagtgcttt ggtttctggt gcccatagcc
     9601 aggttacaac aaaaccagcc aacaacactg ccgccaggag caataaggtg acttgcattc
     9661 cccactgcgc cagcacccac ggcagcaggc cagtacttac cgccgccccc aaccgactca
     9721 tggcggtggc aaaaccgacg cccagcgagc gaatgtcagt aggaaaactt tccgcaggca
     9781 aaatgcccac cagattactg actgccgaaa tggtggtgct gaagagaaca aaagcagcag
     9841 cgttaatgaa ctgccggaag gcaaacaggc catcactacc agcgttgccg ccagcagcaa
     9901 aaaacttccc agcaaaaatt tgcgatgtgc cagcaggtgc gtcagaacta atcccagcag
     9961 cgcgcccaca attaacaacg cattaagcat caggctggca gtcagcgcat cttccagtcc
    10021 aatagtctgg gcgatagttg gcagccaggt ataaatcaca aaccatggga ttacgaggca
    10081 gacaaagaag acgctgttaa acgccgtgcg ccgccagtag cgcgaagaga acaaggtttt
    10141 gatgtgttta tgggtcgccg ttgccacttc atcgcccagt aaaacatggg gaccaaaata
    10201 gcgatgcacg atagcgtgag cttctgcaaa acgcccctgg cgcagtagcc agcgtggcga
    10261 ttctggcgtt ccccagcgta ataacgtaat caacaacgcg ggcagagctg ccgatgccag
    10321 cagccagcgc caggcctccg ggttttcgga aataaagtga tgtccggcaa tacttgccaa
    10381 cacatagcct acggtccaca ccacgctgaa tgcgcccagc aaaacaccgc gatggcggcg
    10441 cggggaaaat tcagccagca aggtgtgacc tactgagtaa tcgcctccca gaccaatgcc
    10501 gatcaaaatg cgcagtccaa taagatgctc tggcgtggtg gcaaaaaatt gcaagaacga
    10561 agcgagcgta atcagcaaaa agctgaaggt gaagattttt tgccgaccaa tatggtcgga
    10621 gatccaccca agaaccaggc tgccaagaaa caaaccaagg tgcgccgagc cgccgatcat
    10681 tcccgccata aacggcgtca gttgcatggc gggcgtaagt tgaataatgg cgtaaccaat
    10741 gacgccgaga acataaccgt cggtcaggtg tgcgccgaaa gtgagcgcag caattcgaca
    10801 gtgaaaacgg ttaagcggta aatcatccat tcgcaccggt gaagtgttca tctttacccc
    10861 ttcattgtcc ggtaataaga acggcttctc gcctgagaag ccgaaaaggc aacgccccat
    10921 catcgcggaa cgctgcctga cacattattt ttcaatcgga tagatagtac ccgtattcat
    10981 aatgccatta ggatcgaact gctttttcag cccttccagc aacgcccacg cgctgccgtg
    11041 ttccagcttg ctccagtgaa cgcgatgttt accgataccg tggtggtgca ccatcgaacc
    11101 accgaggcga atggtttctt cgcaaatgat tttgttgagc ggattgtggt acttgtcgat
    11161 ttcctcttcc ggcttacagt tgacaacgtt gtagtcgtag acgaagtaca tgttggtgcc
    11221 gttctgatag ctatgagagg agtgaccgcc cagcatggtg atgtcgtcgg cgtgcgggaa
    11281 ctcggtacga atacggttaa taacgctttc gtagatttcg tggatgcagc tccagcagcc
    11341 ggacacttcg gtagtaaagc ccatgttgcc ggttttgagg atctgcacac gttcggcagc
    11401 cactttatcc ggtccccagt tcaggttgtt aaaccaggtt tcgatcagct tgctgtccac
    11461 gcgttggcat tgcgggtagc gggcaacgat ttccgcaatc ccttcgcccg tcgccttcgc
    11521 aatgcgaggg ttaccttcag ccataaagat cagcacgcat tttccgtcgg caaaatgggt
    11581 gaagtgttgg gtgccatctt cagcgtcata caaacgagcg atcgacggac gatacccttc
    11641 caccatgatt tcacgcagga tgttgaagcc ggttttcatg tcttccagga tatagccgta
    11701 gaagaggttg ttttccgggg tgaatttaaa gattttcact gttacttcag tgatatagca
    11761 caatgcacct tcgttgccga tgatgatgtg acgaatgtcc gggccagccg cgcggcgtgg
    11821 cacgttttta atgcgtgtga cggtgccatc tgccaacact gcctccagac caacgaccat
    11881 atcttcgatt gcgccgtaga gtgtggagaa ctgcccgata ctgcgggttg ctaccaggcc
    11941 gcccatctgc gccagcggct ttgactgcgg agaatgcccc gtggtgtaac ctttttcacg
    12001 caacgcgttt tccagcacct ccagcggaac accacattgc gccgtcgcct gcatattctc
    12061 aatatcaata ttaatgattt gattcatggc ggaaccgtcg agcaccaccg agttttctac
    12121 aacagtttcc agcccacctt cggtggcgga agcaccggta cgcggcacac cgttaatttt
    12181 gtgcgcattc ataaaattca gcacacggga tacttgctct gtggaaccga gttttacgac
    12241 cgctgccgga atcggcaaag tataaatgcc atgaatatcc ggaaatttac gaaaacggtc
    12301 aatactgttc ttctttaata ctgtttcatc ggtaattacg cgatctgcac caacaatttc
    12361 ctttagctgg tcgacaatcg ctgcgcgaga taaagacata gtaattcctt cctgataata
    12421 aaaatggaat aatcattaat aagagaaaac agaaaccacc gttatgggtt agcgcactaa
    12481 ataaccgcca tcgaccacta ataaatgccc gttgacataa ttcgatgcct gacttgcgag
    12541 aaacacggct gcgcccatta aatcctgagt atcgccccaa cggtttgccg gaatatgatc
    12601 aagaacgcgc tgattggttt ctggattact gcgtgtcgcc agcgtaatgt ctgttgcata
    12661 atagccaggg gcgataccat ttacctgaat attatattga cctagttcat cacaataagc
    12721 tttggtgaat ccggcaagag catgtttagt agcagaatat gcaggtgacc attgtccacc
    12781 taagtaagag aacaatgaac agatattaat gattttaccg cttttctgcg ggatcataat
    12841 ttttgccgct tcgtagctta actcgaatgc ggcggtcagg ttcacatcaa tcatcgggtc
    12901 ccagtcggca cgaccgaagt ccagcacctt attcagctta caaataccgg cattgttaac
    12961 cagaatatca actgtaccga aacgctcaca gcaggcagcg ataatcttct gcggcgcgcc
    13021 ttctgcggtg atatccacct gcatgaagtc cacctcaaca ccctgatttt caatcatttc
    13081 ctttgtttcg ccgttatctt tgacaaaact tggaataaag acatttgcgc ctgctttggc
    13141 caacgccatg gcaaatgcct ggcctaaacc gctattcccg ccggtaacaa ttgcggtttt
    13201 acctttcagg gagaaaaaat ccattgagaa cgcattgaga gattcgattg acatagttag
    13261 ctccaatttc cgtaaggcaa aaaaaaagaa aggtaacttc ccccgcgaga gggaagttac
    13321 ctttctcatt atctctggta actggagata atttttagca taaggacata actgctaatg
    13381 tgatcgtaat cacagtgtga tattcgtatt atcaaatagt tttaaaaagc agaa
//
LOCUS       DBFIFI010000111        13399 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.111, whole genome shotgun sequence.
ACCESSION   DBFIFI010000111 DBFIFI010000000
VERSION     DBFIFI010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.111"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(106..810)
                     /locus_tag="N7B39_003080"
     CDS             complement(106..810)
                     /locus_tag="N7B39_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HGU2423020.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDASNLRKYSILCALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(946..1398)
                     /gene="moaE"
                     /locus_tag="N7B39_003081"
     CDS             complement(946..1398)
                     /gene="moaE"
                     /locus_tag="N7B39_003081"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HGU2423021.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFIGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(1400..1645)
                     /gene="moaD"
                     /locus_tag="N7B39_003082"
     CDS             complement(1400..1645)
                     /gene="moaD"
                     /locus_tag="N7B39_003082"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HGU2423022.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVSGG"
     gene            complement(1638..2123)
                     /gene="moaC"
                     /locus_tag="N7B39_003083"
     CDS             complement(1638..2123)
                     /gene="moaC"
                     /locus_tag="N7B39_003083"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HGU2423023.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(2126..2638)
                     /gene="moaB"
                     /locus_tag="N7B39_003084"
     CDS             complement(2126..2638)
                     /gene="moaB"
                     /locus_tag="N7B39_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HGU2423024.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(2660..3649)
                     /gene="moaA"
                     /locus_tag="N7B39_003085"
     CDS             complement(2660..3649)
                     /gene="moaA"
                     /locus_tag="N7B39_003085"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HGU2423025.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(3637..3781)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            4046..4954
                     /gene="yvcK"
                     /locus_tag="N7B39_003086"
     CDS             4046..4954
                     /gene="yvcK"
                     /locus_tag="N7B39_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HGU2423026.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDAHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHSALEKALQALG"
     gene            complement(5145..7166)
                     /gene="uvrB"
                     /locus_tag="N7B39_003087"
     CDS             complement(5145..7166)
                     /gene="uvrB"
                     /locus_tag="N7B39_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HGU2423027.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(7745..8422)
                     /gene="bioD"
                     /locus_tag="N7B39_003088"
     CDS             complement(7745..8422)
                     /gene="bioD"
                     /locus_tag="N7B39_003088"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HGU2423028.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAAGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQRNSSLQLDYATVNPYTFAEPTSPHIISAQEGRSIESSVMSSG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            complement(8415..9170)
                     /gene="bioC"
                     /locus_tag="N7B39_003089"
     CDS             complement(8415..9170)
                     /gene="bioC"
                     /locus_tag="N7B39_003089"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HGU2423029.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRQSADVLLAMLPQRKYP
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHRIQPITLWFDDALSAMRSLKGIGATHLHEGRDQRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIVRE"
     gene            complement(9157..10311)
                     /gene="bioF"
                     /locus_tag="N7B39_003090"
     CDS             complement(9157..10311)
                     /gene="bioF"
                     /locus_tag="N7B39_003090"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HGU2423030.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFVHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     TCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSDEGDARREKLAALITRFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPSGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(10308..11348)
                     /gene="bioB"
                     /locus_tag="N7B39_003091"
     CDS             complement(10308..11348)
                     /gene="bioB"
                     /locus_tag="N7B39_003091"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HGU2423031.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMSYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            11435..12724
                     /gene="bioA"
                     /locus_tag="N7B39_003092"
     CDS             11435..12724
                     /gene="bioA"
                     /locus_tag="N7B39_003092"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HGU2423032.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQHFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            12783..13259
                     /locus_tag="N7B39_003093"
     CDS             12783..13259
                     /locus_tag="N7B39_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HGU2423033.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
BASE COUNT         3096 a         3643 c         3441 g         3219 t
ORIGIN      
        1 cgctcggcca taaggcgtat tttatacctg gtacttgtta agtgtcgttt ttagtaagaa
       61 aaaaaatccc gacacatggc cgggatattg atggtgaaaa aagaattaac gacgattgcc
      121 aaaaattcgc agcaacatca ggaacaggtt gatgaagtcc agatacaagg ttaatgcgca
      181 aagaatggaa tatttgcgca ggttcgacgc gtcgcgggta tctatctgct cacccatatt
      241 tttcagtttc tgggtgtcat acgccgtcaa tccgacaaag acaatcacgc cgatgtaggt
      301 gactgcccac atcaacgctt cgcttttcag ccagaagttg accagcgatg ccaacacaat
      361 gccgattaac gccataaaca gcatattgcc aaagccactt aaatcgcgct tcgtggtgta
      421 accgtacagg ctcattgcgc cgaacatccc ggcagtaacg acgaaggtac tggcgataga
      481 agcagcggta taaacaatga aaatactgga aagcgtaaga cccgtcagcg ccgaataaag
      541 cataaagagc atcgtcgtta cacctgcgct cagcttttga atcatcgctg ataacacaat
      601 aaccaatgct aattgcgcga tgatcagacc gattaaaaag acacggttag tgaacaacag
      661 ctccatcacg gccgcggaat tagccgcata ccaggcaaca aatgcggtca gcaataagcc
      721 aacggtcatc cagccataga cttgcgccat ataagtctgc aagccagccc ggggttgtac
      781 aattgaatca gaacgtggga atctgtccat gacgatctcc tgaagatata aggaatatct
      841 taaggatact gcaaaatgat gaggctgtgc atcgacgcag cgtaaacgca tgtactgagc
      901 ggtgaaattg ccggacgcag cggtgcctta tccggctaac aaaaactacc agcgttttgc
      961 cgcctgctga tcgctctccc gagcttcgac ccagcggtcg ccctccggcg tggcttcgcg
     1021 cttccagaac ggtgcgcggg ttttgagata atccataata aactgcccgg cttcaaacgc
     1081 actgctgcga tgcgcactgg tgacaccgat aaaaacgatt tcatcgcccg gccataattc
     1141 cccgatgcgg tgaatcaccg tgacgcgccc cagcggccag cggttacgcg cttcatcgac
     1201 aatttctgcc agtgcttttt cagtcatccc cggatagtgt tcgagggtta atgctttgac
     1261 gctgtcgccc aggttatggt tgcgcacctt accggtaaag gtgactaccg caccgtcttc
     1321 gtcacgctcc gccagccacg ggtactcttc tcctacgctg aacggctgcg gaccaacaac
     1381 aattttggtt tctgccatct taacctccgc ttaccggcgg gaagaaagct acttcgtcgc
     1441 cgtcagtcag cggatggtca aaactcacca gcgtctggtt gacggcagcc agtaatttgc
     1501 catcttccag cgccagcgcc cagcgatcgc tctgcgcagc caggtgctgg cgtaacgctt
     1561 caacggttgg gaaatccgca gccacttcgg ttgcatctgt tcctaccaac tcgcgcacct
     1621 gggcgaaaaa aagaacttta atcatccgct tccaccttaa agtcacccga cttgccgccg
     1681 ctcttcgcca gcaaacgtac cggaccaatc accatatctt tttgcaccgc tttgcacatg
     1741 tcataaatgg tcagcgccgc cacggaggcc gcggttaacg cttccatttc gacaccggtt
     1801 ttcccggtca ggcggcataa ggtttctata cgcacccgat tgtgctccgg ctcggcctgt
     1861 aaattgactt caactttgct gagcatcagc ggatgacaca gcgggatcag atcccaggtg
     1921 cgttttgccg cctgaatacc ggcaatacgc gcagtggcaa atacgtcgcc tttgtggtgg
     1981 cgaccatcaa taatcatcgc cagcgtctcg ctgcgcatgg tgacaaaggc ttccgcccgc
     2041 gcttcacgca cggtttccgc tttggcggag acatccacca tgtgcgcttc gccagcggcg
     2101 ttgatatggg tcagttgcga catacttatt tcttcaaatg tggatggaaa ttacacggac
     2161 gcgtacgggc atccagctgc ggcgcgatga tattttccca tgcggtacgg cacgctttgg
     2221 tcgaacccgg catggcgaaa atcagcgttt tgttagcgac gcccgctacc gcacgagatt
     2281 gcaacgtgga agtgccaatc tcttcaaacg acaacatacg gaacacttca ccaaaacctt
     2341 caacttcacg gtcgaacaac ggcagcaatg cttcgggagc ctgatcacct tccgtcaggc
     2401 cagtcccccc cgtaatcaac accacttgta catcgtcgct ggcgatccac gcagatacct
     2461 gagcgcgaat agcgtagcgg ttttctttca caatggcttt atcgacaacg tgatggcccg
     2521 cttcttgcgc cgaatcgcgc aaatagtgac cggaggtatc gtcttcttca ccgcgacgat
     2581 tagaaaccgt aagaatagca atacgggtcg ggataaattc agtgcttacc tgactcatct
     2641 gatctctcct tttgacgttt tagccgccaa tgtacgataa gttttgcgta ataccggtgt
     2701 tgttttgatg caggaaatgg gtctgtttct tctcccgcag cgccgctgaa atacgcgctt
     2761 ccagcgcctg ttgctgggta tcgtcttcca gcagatcgcg caggttaacg ccgccttcac
     2821 caaacaggca gagatggagt ttaccaatgg aggaaacgcg cagacggttg caagtggcgc
     2881 agaagtcttt ttcatacggc atgataaggc caatctctcc ggcgtaatcc ggatgacaaa
     2941 agacttgcgc gggaccgtcg ctgcgttgac gtaattggtg gatccagccg cgacgcagta
     3001 gctcgtcacg cagaacctga ccagagatgt gatgcttacg gaaaagctcg ctgccctcgc
     3061 ccgtttccat cagttcgatg aaacgcagct ggatagggcg atgctggatc cagttcagaa
     3121 aggtgtcgag ctggtgatga ttcacatcac gcatcagcac ggtattgact ttgaccttct
     3181 caaaaccggc ctcaaatgca gcatcgatcc ctgccatcac ctgattgaat ttatcctggc
     3241 cggtaatggc atgaaactgg cgggcgtcca gactgtcgac gctgacgtta atgccagtaa
     3301 gtcccgcatc gcgccagttc gccacatcgc gttccagacg gtaaccattg gtggtgaccg
     3361 ctatctggcg aatagcgtcg ttttctcgca cagcggcgat gatatcggta aagtcgcggc
     3421 gtaaagacgg ctcaccgcct gtcaggcgca ctttttcggt gcccagactg gcgaaggcgc
     3481 gcgtaacccg gcgaatttca tcgacggtca gaaagccttt attggtgacg ccgctcggtt
     3541 tgtagccatc cggcaggcag taggtgcaac gaaagttaca cacatcggta atcgacaggc
     3601 gcaagtagta aaacttacgc gcaaatgcat cagtcagttg tgaagccatg tacacctttc
     3661 cagatacggg aggcgaagtc atttcttcct tcgccctggt ggcaatcttt acgagcattg
     3721 tcacggccaa agcaccatat cagttgaccc aggtgcagag gctagagtgt ttagtggtta
     3781 agccgatact agcgtgtaaa tgagattttt accatccaca ctttcgctat gtaatcatgt
     3841 atatagcgtc ctgatcgtgc tatttcgcta cagaataagg gaaaatggcg gttttacttt
     3901 gatatacatc atcagggagg catagcgtgc atcgtccttt agaggtagaa gaaaatagtt
     3961 tgtatcctca atattggcag gttaattgct gtttccccgc aatttgcgct actgtagcgc
     4021 ggataatctg actccaggaa actatatgcg caatcgtacg ctggctgatc ttgatcgtgt
     4081 cgttgctctc ggcggagggc atggactggg acgcgttctc tcatcacttt cgtctttggg
     4141 ttctcgttta acgggtatcg tcaccaccac ggataatggt ggctcgacgg ggcgtattcg
     4201 ccgttcagaa ggcggcattg cctggggcga tatgcgcaac tgccttaacc agctgataac
     4261 tgaacccagc gtcgcttcag caatgtttga atatcgtttc ggcggtaatg gcgaactttc
     4321 cgggcacaac cttggaaacc tgatgttgaa ggcactggat caccttagcg tgcgacctct
     4381 tgaagcgatc aatctgattc gaaatctgct gaaagtggac gcacatttga ttccgatgtc
     4441 ggagcatcct gttgatctga tggcgattga cgatcagggg catgaagttt acggcgaggt
     4501 caatatcgac cagttaacta cgccgattca agagttattg ttaacgccta atgtacccgc
     4561 aacgcgtgag gcggttcacg ctatcaatga agcggatctc atcattattg ggcctggcag
     4621 tttttacacc agcctgatgc caattctgct gctgaaggaa atcgcgcagg cattacgccg
     4681 cacgccagcg ccgatggtct atatcggcaa tctggggcgt gagttgagtt tacctgcggc
     4741 caatttgaag ctggaaagca agctggcaat tatggagcag tatgttggta aaaaggtcat
     4801 tgatgcggtc atcgtcgggc caaaagtgga tgtctcggcg gtgaaagagc ggattgtgat
     4861 tcaggaggtg ctggaggcca gcgatattcc gtatcgtcat gaccgccagt tgttacatag
     4921 cgcactggaa aaggcgttac aggctttagg ttaacggaaa tccgaaggaa aattccggct
     4981 tcctaatgaa gacaaagtgc gcgttgttta tgacggatgc ggcgtgaacg ccttatccgg
     5041 cctacaaacc gcgctaattc aatgtattgc agaatcatgt aggcctgata agcgtagcac
     5101 atcaggcaat tttgcttttg tcatctgtct tcttcgttat cctgtcacga agccgcgata
     5161 aacaattcac gcagctgatg caactggtca cgaatttgtg ccgcttcttc gaactccaga
     5221 ttttgcgcgt gttgcatcat taacccttcc agctcatgga ttttctgttg caacgcttta
     5281 ggcgacatat ccatcggcac attatccggc tcaacaatcg gtcgcgattt tcctctgccc
     5341 ttcgctttgg ttttagcaat gttctgcccc aacgccagga tatcgaccac tttcttgttc
     5401 aagccttgcg gcgtaatgcc gtgttcctcg ttgtacttct gctgtttctc gcggcgacgt
     5461 tcggtttcgc caatcgcttt cgccattgac ggggtgatct tatcgccgta gagaatcgct
     5521 ttaccgttaa cgttacgcgc cgcacgacca atagtctgga tcaacgaacg ttcagaacgc
     5581 aggaagcctt ctttgtcggc gtcgaggatc gccaccagcg aaacctccgg catatccaga
     5641 ccttcgcgca gtaagttgat ccccaccagc acgtcgaact cgcccagacg caagtcgcgg
     5701 ataatctcca tacgttcgac ggtgtcgata tctgagtgaa gataacgcac gcgctcaccg
     5761 tgttcttcga gatattcggt aagatcttcc gccattcgct tggtcagtgt ggtgaccagt
     5821 acgcgttcgt taattgccgc tcgctgacga atctccgaaa gaagatcatc aacctgtgtc
     5881 gccactggcc gcacttcgat aatcggatca agcaatccgg ttggacgcac cacctgatcc
     5941 accacatcgc cgccggattt ttccagctca taattacctg gcgtcgccga aacatagatg
     6001 gtttgcggcg ctaatgcttc gaactcttca aatttcagcg gacggttatc cagcgctgat
     6061 ggcaggcgga agccgtactc caccagtgtc tctttacgcg cccggtcacc gcgatacatg
     6121 ccgccaattt gtggaatggt gacgtgagat tcatcgacca ccagcagccc atcggcaggc
     6181 aggtaatcaa acagcgtcgg cggtggctca cccggtccac gaccggagag gaagcgcgag
     6241 tagttttcaa tccccgaaca gtagcccagc tcgttcatca tctccagatc aaactgggta
     6301 cgctgggtca gccgctgctc ttccagcagt ttgttgtttt ccaatagcac tttgcgtctg
     6361 gcggccagct cttctttgat ctcctccatc gcctgtacga tgcgctcgcg cggtgtgacg
     6421 tagtgcgttt tcgggtagat ggtaaaacgt ggaatggtgg aaacaatctg cccggttagc
     6481 gggtcaaata acgacaatcg ttccacttcc tcgtcaaaca gttccacgcg aagtgcaatg
     6541 tcatccgatt ctgccgggaa gatatcaatc acctcgccac gaacacggaa agtaccacgc
     6601 tggaatgctt gatcattacg agcgtattgc agctccgcca gtcggcgcag aatcgcgcgc
     6661 tgatcgataa tcataccgac cgtgagatgg agcatcatct tgagatataa atcaggatcg
     6721 cccagaccat aaatcgcgga aacagacgcg accacaacaa catcacgccg ctccagcatc
     6781 gctttggtgg cggacaaacg catctgttcg atatgttcgt ttaccgaggc atctttctca
     6841 atgaaggtgt cggaactcgg tacataggct tctggctgat agtagtcgta gtaggagacg
     6901 aaatattcca ccgcgttttc cgggaagaac tctttcattt cgccatacag ctgggccgcc
     6961 agcgttttgt tgggcgcaag taccatggta gggcgctgaa ggtcagcaat gacattggca
     7021 atggtgaagg ttttcccgga accagtcaca ccaagtaacg tctggtgcgc caggccatct
     7081 tccagcccct cttcgagacg tcgaatcgcc tctggctgat cgccagaagg tttaaaagcg
     7141 gaattcagtt tgaacggttt actcatgagt cgctacctga aggagttggg cgggcaggta
     7201 tgtaatttta ctcgtcgtac ttaattatgc caacaaatta tactggataa aaaaacagtt
     7261 catcaccata atatttctgt tacagcgtaa actccgctgt caatcatgag taaaatttac
     7321 tctgtggcga gataaaactc ggaccttacc gggttatccc caaagcaacg gcttttttaa
     7381 catttgtcaa gatgagtgat gtcagtttta tggcaagaga tgcctgttca gtgactgcca
     7441 ttgcaattat ctaaccagtt aaaaaataaa agatatttct ttgagccgtc tttaacgcca
     7501 attcgcgtgc aagccgcgta ttctctcgct tgcctcgtgt tttctaactc taatacacat
     7561 ggttatccac aggaatagtg gataactgtc tgcagcccct atcccccgcc gcctgggcaa
     7621 ttcccacatt cgccgcatga tgcggaagta aaattttttt cgtgattatt ttagaattta
     7681 attggttaaa ttgcagtcaa tcgaagacgc gatctcgctc gcaatttaac caaatacagg
     7741 atggctacaa caaggcaagg tttatgtact ttccggttgc cgcattttct ggattttctg
     7801 caagccaggg gatctctccc agcagcggcg cgggaatcat gcgggtgagc gtggtcatat
     7861 attcagcgtg acgttttccc ggaggcgtga catcgttcgc cacccaaccc gccagagtca
     7921 gcccggcgtg ttgtatcgcc tgtgccgtca acatcgcgtg attaatacag ccaagtttca
     7981 cgccaaccac cagtattacc ggcagttgtt cctgtgttac ccaatccgca aaagtgaaag
     8041 tgtcagaaag cggcgtaaac cagccgccag cgccttccac taacacccag tcagcctgtt
     8101 gttcaagcgc gcgtaatccg gagctcatta ccgatgattc tatcgacctg ccctcttgcg
     8161 cgctgatgat gtgcggtgag gtgggttctg cgaaggtgta aggatttacc gttgcgtaat
     8221 ccagctgcag gctgctgttg cgctgtaacg ccagcgcgtc actattacgt aaaccctccg
     8281 gggtcttttc gctgccagag gcgaccggtt tataacctgc cgtccgatag cctgctgcct
     8341 ttgcggcttg taaaagtgca caactggcga cagttttccc cacttcggta tccgttccgg
     8401 tgacaaaata acgtttactc acgaacaatc actcccaaaa aaagatgata cgtcagagga
     8461 tatcgcccct gttgttgcgg ccaggccagt tgcaatcgct gcaactgtga acgcgttaat
     8521 attcgctggt cgcgcccttc atgaagatgc gtggcaccga tgcctttcag cgaacgcatg
     8581 gcactgagcg catcatcaaa ccatagtgtg atgggctgaa tacgatgttg ataatgcacg
     8641 ccgttcagcg actgttcgat ttcatctggc ggtaaaaagc gattagcatg cgggcgctcg
     8701 tccaccgcct gccacgcctg atgcagttcg ggtaacgatc cctgcaccag cgtggtaaac
     8761 gcgaccacgc ctccggggcg caccacccga tacagctcgc ggagtgccgt tgataaatta
     8821 ccgcaccact gcaccgcgag attgctccat gcaagatcga acgtcgcagt cgctaacggc
     8881 agggattcga tatctcccgc cagataatgg tctgcagcat ctttctggcg ggcttgcgca
     8941 agcattggcg gcgagagatc taacgccgtc acctgcgcgt gacgctctcg ccagtggcgg
     9001 ctcatccagc caggcccaca ccccgcgtcc agtacgtggg ggtatttacg ctgtgggagc
     9061 attgccagta agacatcagc actctggcgc tgtaactctg cgtgttgctc atagtgcgcg
     9121 gctgcccgac caaatgccgc tgcaatggct tgtttattaa ccgttgccat gcagcacctc
     9181 cagcagacgg tcgatatcct gcatttcatg cgcggcggtt agcgttaagc gcagtcgcgc
     9241 agtaccagag ggtacggttg gcgggcgaat cgccgtgacc cagcagcctt gctggcgcag
     9301 tttttctgcc agttgtaacg cacggctgtt atcaccgacg atcaatggct ggatggcgct
     9361 gcatgaatca gcaagcgtaa acggcaaatc ctgtactccg gcacgaaaac gcgtaatgag
     9421 tgccgccagt ttttcgcgcc gtgcatcacc ctcatcactg cgaatgaccg ccagtgacgc
     9481 acgtaatgcc tgcgcctgag cgggcggcat actggtgctg tagataagat ggcgggcgaa
     9541 ttgcagcaga taatccgcca ccgtactgga gcaaagcacc gcagccccgc tgacgccaaa
     9601 tcctttgcca aaagtcacta ccagcaattc tggttttacc ttttgcaacc agcaggtgcc
     9661 gcgcccctgc tccccgataa cgcccgtgcc gtgggcatca tcgaccatca accagccatt
     9721 gtgctgttgc gttacctgct ggatttccgc cagtggcgca ctatcgccgt ccatgctgaa
     9781 cacgccttct gtcaccacca gttgctgccc cggacagggg gaagcaagca gtcgcgccag
     9841 atgagtgaca tcgttatgaa caaaacggcg aagctgcgac gggcttaaac tggcagcctc
     9901 cagcaatgag gcatggctaa gccggtcagc gacaatgcgg tcctcttttg ccatcatcgc
     9961 ggcaatgacc gcctgattag cggcaaaacc agagataaat agcagtgccc gcgaatagcc
    10021 cagccactcg gccagttctt cttccagcgc ctgatgcgca acgctataac cgctgacgtg
    10081 accggaaccg ccgctaccga cgccaaattg ctccgcaccc tgcttccagg cacggataat
    10141 ttgcggatga tggcttaaac ctaaataatc gttactggaa aagttcagat actggcgatc
    10201 gtccgccacc agccagcgtc cggctccttg cgccactgga taacggcgac gcagggcatc
    10261 ggcagcacgc cgcgcatcga gcgccgcgtt gattttctcc tgccagctca tagtgctgcc
    10321 gcgttgtaat attcgtcagt gtccggggtc atcagtgcct gctccagacg ctgctgttgt
    10381 tcgttatccc ctgccagcac ggcagtttgc tgcggattta gccccagttt gcgaaacagt
    10441 tgcaggtctt tatcttcttc cggattgggc gtggttagca gtttgcaacc gtagaaaatc
    10501 gagtttgcgc ctgccataaa gcacatcgcc tgagtctgtt cgttcatctg ctcgcgtccg
    10561 gcagaaaggc gcacgtaaga ggtcggcatc atgatccgcg ctacggcaat ggtgcgaata
    10621 aaatcaaagg catcgacatc atcgttatct gccagtggag tacctttcac cttcaccagc
    10681 atgttgattg gcacgctttc cggcggcgtc ggcaggtttg ccagttgcag taataatccg
    10741 gcgcgatctt ttaccgtttc gcctaaaccc acaatgccac ctgagcagac tttgatcccg
    10801 gcatcgcgca ctttttccag cgtatcaagg cgttcctgat aagtacgtgt ggtgatgata
    10861 ttgccgtaaa actccggcga ggtgtcgagg ttatggttgt agtaatccag cccggcattc
    10921 gcaaggcgct gcgcctgaga ttcactcaac gtacccagcg tcatacacgc ctccagcccc
    10981 atcgctttta ctccctgcac catttgttcc aggtatgaca tatcgcgttc gtggggattc
    11041 ttccacgccg cgcccataca gaagcgcgtc gatcctgccg ctttcgcttt gcgcgccgac
    11101 tccagcacct gttcaacttc catcaaccgc tcggcttcca gcccggtttt gtagcgcgag
    11161 ctttgcgggc agtatttgca atcttccgga caagctccgg tcttaatcga cagcaaggtg
    11221 ctgacctgca cctgacgagg atcgaaatgc tggcgatgca cctgctgcgc ttcaaacagc
    11281 agatccagca acggtttttc aaataattct gtgacttgcg acaatgtcca gcgtgggcgg
    11341 tgagccatgg ggcttctcca aaacgtgttt tttgttgtta attcggtgta gacttgtaaa
    11401 cctaaatctt ttcaatttgg tttacaagtc gattatgaca acggacgatc ttgcctttga
    11461 ccaacgccat atctggcacc catacacatc catgacctcc ccgctaccgg tttatccggt
    11521 ggcgagcgcc gaaggttgcg agctaatttt gtctgacggc agacgcctgg ttgacggtat
    11581 gtcgtcctgg tgggcggcga tccacggtta caatcacccg cagcttaatg cggcgatgaa
    11641 gtcgcaaatt gatgccatgt cgcatgtgat gtttggcggt atcacccatg cgccagccat
    11701 tgagctgtgc cgcaaactgg tggcaatgac gccgcaaccg ctggaatgcg tttttctcgc
    11761 ggactccggt tccgtagcgg tggaagtggc gatgaaaatg gcgttgcagt actggcaagc
    11821 caaaggcgaa gcgcgccagc attttctgac cttccgcaat ggttatcatg gcgatacctt
    11881 tggcgcgatg tcggtgtgcg atccggacaa ctcaatgcac agcctgtgga aaggctatct
    11941 gccagaaaac ttgtttgctc ccgccccgca aagccgcatg gatggtgaat gggatgagcg
    12001 cgatatggtg ggctttgccc gcctgatggc ggcgcatcgt catgaaatcg cagcggtgat
    12061 cattgaaccg attgtccagg gcgcaggcgg gatgcgcatg taccatccgg aatggttaaa
    12121 acgaatccgc aaaatgtgcg atcgcgaagg tatcttgctg attgccgacg agatcgccac
    12181 cggatttggt cgtaccggca aactgtttgc ctgtgaacat gcagaaatcg cgccggacat
    12241 tttgtgcctc ggtaaagcct taaccggcgg cacaatgacc ctttccgcca cactcaccac
    12301 gcgcgaggtt gcagaaacca tcagtaacgg cgaagccggc tgctttatgc acgggccaac
    12361 ttttatgggc aatccgctgg cctgtgcggc tgcaaacgcc agcctggcga ttctcgaatc
    12421 tggcgactgg cagcaacagg tggcggctat tgaagtacag ctgcgcgagc aacttgcccc
    12481 cgcccgtgat gccgaaatgg ttgccgatgt gcgcgtgctg ggggccattg gcgtagtcga
    12541 aacaactcgt ccggtgaata tggcggcatt gcaaaaattc tttgtcgaac agggtgtctg
    12601 gatccggcct tttggcaaac tgatttacct gatgccgccc tatattattc tcccgcaaca
    12661 gttgcagcgt ctgaccgcag cggttaaccg cgcggtacag gatgaaacat ttttttgcca
    12721 ataacgggaa gtcagcgtga gagtttctgg ctacactttc tgcaaactag aaaggagggt
    12781 ttatgaaact catcagtaac gatctgcgcg atggcgataa gttgccgcat cgtcatgtct
    12841 ttaacggcat gggttacgat ggcgataata tttcaccgca tctggcgtgg gatgatgttc
    12901 ctgcgggaac gaaaagtttt gttgtcacct gctacgaccc ggatgcgcca accggctccg
    12961 gctggtggca ctgggtagtt gttaatttac ccgctgatac ccgcgtatta ccgcaagggt
    13021 ttggctctgg tctggtagca atgccagacg gcgttttgca gacgcgtacc gattttggta
    13081 aaaccggata cgatggcgca gcgccgccga aaggcgaaac ccatcgctac atttttaccg
    13141 ttcacgcgct ggatgtagag cgtattgatg tcgatgaagg agccagcggc gcgatggtcg
    13201 ggtttaacgt tcatttccac tctctggcaa gcgcatcgat taccgcgatg tttagttaat
    13261 cactctgcca gatggcgcaa tgctatctgg tatcacttaa aggtattaaa aacaactttt
    13321 tgtcttttta ccttcccgtt tcgctcaagt tagtataaaa aagctgaacg cgaaacagca
    13381 aaacccaata atatcaatg
//
LOCUS       DBFIFI010000112        13384 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.112, whole genome shotgun sequence.
ACCESSION   DBFIFI010000112 DBFIFI010000000
VERSION     DBFIFI010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13384
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.112"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            58..4020
                     /gene="putA"
                     /locus_tag="N7B39_003094"
     CDS             58..4020
                     /gene="putA"
                     /locus_tag="N7B39_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HGU2423034.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGYVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGRGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVIDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSTVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(4060..4698)
                     /gene="rutR"
                     /locus_tag="N7B39_003095"
     CDS             complement(4060..4698)
                     /gene="rutR"
                     /locus_tag="N7B39_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HGU2423035.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKSLIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            4986..6077
                     /gene="rutA"
                     /locus_tag="N7B39_003096"
     CDS             4986..6077
                     /gene="rutA"
                     /locus_tag="N7B39_003096"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HGU2423036.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPVLTQEVA"
     gene            6077..6769
                     /gene="rutB"
                     /locus_tag="N7B39_003097"
     CDS             6077..6769
                     /gene="rutB"
                     /locus_tag="N7B39_003097"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HGU2423037.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTS
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFVQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            6781..7120
                     /gene="rutC"
                     /locus_tag="N7B39_003098"
                     /pseudo
     CDS             6781..7120
                     /gene="rutC"
                     /locus_tag="N7B39_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309283.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
     gene            7128..7928
                     /gene="rutD"
                     /locus_tag="N7B39_003099"
     CDS             7128..7928
                     /gene="rutD"
                     /locus_tag="N7B39_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HGU2423038.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSITQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDHP
                     ASVTVLVCVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            7938..8528
                     /gene="rutE"
                     /locus_tag="N7B39_003100"
     CDS             7938..8528
                     /gene="rutE"
                     /locus_tag="N7B39_003100"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HGU2423039.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIIACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            8539..9033
                     /gene="rutF"
                     /locus_tag="N7B39_003101"
     CDS             8539..9033
                     /gene="rutF"
                     /locus_tag="N7B39_003101"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HGU2423040.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQN
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            9054..10382
                     /gene="rutG"
                     /locus_tag="N7B39_003102"
     CDS             9054..10382
                     /gene="rutG"
                     /locus_tag="N7B39_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HGU2423041.1"
                     /translation="MAMFGLPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASGFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGSSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(10465..10572)
                     /locus_tag="N7B39_003103"
     CDS             complement(10465..10572)
                     /locus_tag="N7B39_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HGU2423042.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            10944..11540
                     /gene="wrbA"
                     /locus_tag="N7B39_003104"
     CDS             10944..11540
                     /gene="wrbA"
                     /locus_tag="N7B39_003104"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HGU2423043.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMS
                     PQLFEKAGGKTQTAPVATPQELANYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            11561..11788
                     /gene="yccJ"
                     /locus_tag="N7B39_003105"
     CDS             11561..11788
                     /gene="yccJ"
                     /locus_tag="N7B39_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HGU2423044.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(11826..13067)
                     /gene="agp"
                     /locus_tag="N7B39_003106"
     CDS             complement(11826..13067)
                     /gene="agp"
                     /locus_tag="N7B39_003106"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HGU2423045.1"
                     /translation="MNKTLIAAAVAGVVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWRDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDADGFCPMDKFDSVLNEAVK"
BASE COUNT         2985 a         3551 c         3682 g         3166 t
ORIGIN      
        1 catggatatt tcacgataac gttaagttgc acctttcaga acaacaggag taatggcatg
       61 ggaaccacca ccatgggggt taagctggac gacgcgacgc gtgagcgtat taagtctgcc
      121 gcgacacgta tcgatcgcac gccacactgg ttaattaagc aggcgatttt ttcttatctc
      181 gaacaactgg aaaacagcga tactctgcca gagctacctg cgctgctttc tggcgctgcc
      241 aatgagagcg atgaagcacc gactccggca gaggaaccac accagccatt cctcgacttt
      301 gccgagcaaa ttttgcccca gtcggtttcc cgcgctgcga tcaccgcggc ctatcgccgc
      361 ccggaaaccg aagcggtttc tatgctgctg gaacaagccc gcctgccgca accagttgct
      421 gaacaggcac acaaactggc gtatcaactg gccgataaac tgcgtaatca aaaaaatgcc
      481 agtggccgcg caggtatggt ccaggggtta ttgcaggagt tttcgctgtc atcgcaggaa
      541 ggcgtggcgc tgatgtgtct ggcagaagcg ttgttgcgta ttcccgacaa agccacccgt
      601 gatgcgttaa ttcgcgacaa aatcagcaac ggtaactggc agtcacacat tggtcgtagc
      661 ccgtcactgt ttgttaatgc cgctacctgg gggctgttgt ttactggcaa actggtttcc
      721 acccataacg aagccagcct ctcccgctcg ctgaaccgca ttatcggtaa gagcggtgaa
      781 ccgctgatcc gcaaaggtgt ggatatggca atgcgcctga tgggcgagca gttcgtcacg
      841 ggcgaaacca tcgcggaagc gttagccaat gcccgcaagc tggaagagaa aggtttccgt
      901 tactcttacg atatgctcgg cgaagctgca ctgactgccg cagacgcaca ggcgtacatg
      961 gtttcctatc agcaggcgat tcacgccatt ggtaaagcgt ctaacggtcg tggcatctat
     1021 gaaggtccgg gcatttcaat caagctgtcg gcgctgcatc cgcgttacag ccgcgcccag
     1081 tatgaccggg taatggaaga gctttacccg cgtctgaaat cactcaccct gctggcgcgt
     1141 cagtacgata ttggtatcaa cattgacgcc gaagaggccg atcgtctgga gatctccctc
     1201 gatctgctgg aaaaactctg tttcgagcca gagctggcag gctggaacgg catcggtttt
     1261 gttattcagg cttatcaaaa acgctgcccg ctggtgattg attacctgat tgatctcgcc
     1321 acccgcagcc gtcgccgtct tatgattcgc ctggtgaaag gcgcgtactg ggatagtgaa
     1381 atcaagcgcg cgcagatgga cggccttgaa ggttatccgg tttatacccg caaggtatat
     1441 accgacgttt cttatctcgc ctgtgcgaaa aagctgctgg cggtgccgaa tctaatctac
     1501 ccgcagttcg caacgcacaa cgcccacacg ctggcggcga tttatcaact ggcaggtcag
     1561 aactactatc cgggtcagta cgagttccag tgcctgcatg gtatgggcga gccgctgtat
     1621 gagcaggtca ccgggaaagt tgccgacggc aaacttaacc gtccgtgtcg tatttatgcc
     1681 ccggtcggca cacatgaaac gctgctggcg tatctggtgc gccgcctgct ggaaaacggt
     1741 gctaacacct cgtttgttaa ccgtattgcc gatacctctt tgccactgga tgaactggtc
     1801 gccgatccgg tcactgctgt agaaaaactg gcacaacagg aagggcaaac tggattaccg
     1861 catccgaaaa ttcccctgcc gcgcgatctt tacggtcacg ggcgcgacaa ctcggcaggg
     1921 ctggatctcg ctaacgaaca ccgtctggca tccctctctt ctgccctgct caatagtgca
     1981 ctgcaaaaat ggcaggcctt gccaatgctg gaacaaccgg tagcggcagg tgagatgtcg
     2041 cccgttatta acccggcgga accgaaagat attgtgggct atgtacgtga agccacgccg
     2101 cgtgaagtag agcaggcgct ggaaagtgcg gttaataacg cgccaatctg gtttgccact
     2161 cctccggctg aacgcgcggc gattttgcat cgcgctgccg tgctgatgga aagccagatg
     2221 cagcaactga ttggtattct ggtgcgtgaa gccggaaaaa ccttcagtaa cgccattgcc
     2281 gaagtgcgtg aagcggtcga ttttctccac tactacgccg gacaggtgcg ggatgatttc
     2341 gctaacgaaa cccatcgtcc attagggcct gtggtatgta tcagcccgtg gaacttcccg
     2401 ctggcgattt tcaccgggca gatcgctgcc gcactggcgg caggtaacag cgtactggca
     2461 aaaccggcag aacaaacgcc actgattgcc gcgcaaggga tcgccatttt gctggaagcg
     2521 ggcgtaccgc caggcgtggt gcaattgctg ccaggtcggg gtgaaaccgt gggcgcgcaa
     2581 ctgacgggtg atgatcgcgt acgcggggtg atgtttaccg gttcaaccga agtcgctacg
     2641 ttactgcagc gcaatatcgc cagccgcctc gacgctcagg gtcgccctat tccgctcatc
     2701 gctgaaaccg gcggcatgaa cgcgatgatt gtcgattctt cagcactgac cgaacaagtc
     2761 gtaatagatg tgctggcctc ggcgttcgac agtgcgggtc agcgttgttc ggcgctgcgc
     2821 gtgctgtgcc tgcaagatga gattgccgac cacacgttga aaatgctgcg cggcgcaatg
     2881 gccgaatgcc ggatgggtaa tccgggtcgc ctgaccaccg atatcggtcc ggtgattgat
     2941 agcgaagcga aagccaatat tgagcgccat attcagacca tgcgtagcaa aggccgtccg
     3001 gtgttccagg cggtgcggga aaacagcgaa gatgcccgtg aatggcaaag cggcaccttt
     3061 gtcgccccga cgctgatcga actggatgac tttgccgaat tgcaaaaaga ggtctttggt
     3121 ccggtgctgc atgtggtgcg ttataaccgt aatcagctgc cggagctgat cgagcagatt
     3181 aacgcttccg gctatggtct gacgcttggc gttcatacgc gtattgatga aaccatcgcc
     3241 caggtcactg gctcagccca tgttggtaac ctgtatgtta accgtaatat ggtgggcgca
     3301 gtggttgggg tgcagccatt cggcggcgaa gggttgtccg gtaccgggcc gaaagcaggc
     3361 ggtccgctct atctctaccg tctgctggcg aatcgcccgg aaagtgcgct ggcggtgaca
     3421 ctcgcgcgtc aggatgcaga gtatccggtc gatgcgcagt tgaaagccgc attgactcag
     3481 ccgctaaatg cactgcggga atgggcagca aatcgtccag aattgcaggc gttatgtacg
     3541 caatatggcg agctggcgca ggcaggaaca caacgattgc tgccagggcc gacgggtgag
     3601 cgcaacacct ggacgctgct gccgcgtgag cgcgtattgt gtatcgcgga tgatgaacag
     3661 gatgcgctga ctcagctcgc cgccgtgctg gcggtgggca gccaggtgct gtggccagat
     3721 gacgcgctgc atcgtcagtt agtgaaggca ttgccatcga ctgtcagcga gcgtattcaa
     3781 ctggcgaaag cggaaaatat taccgctcaa ccgtttgatg cggtgatctt ccacggtgat
     3841 tcggatcagc ttcgcgcatt gtgtgaagca gttgccgcgc gggatggcgc aattgtttcg
     3901 gtgcagggtt ttgcccgtgg cgaaagcaat atcctgctgg aacggctgta tatcgagcgt
     3961 tcgctgagtg tgaataccgc tgccgctggc ggtaacgcca gcttaatgac gatcggttaa
     4021 acaggtagcc ggaggatgtt acaccctcct ccggcatcat tagcgtggtc gaatcccctc
     4081 aataataatc cgctgcacgt tttcaaccgt ttgattgaaa aatacctcat cgcgcaacgt
     4141 tgcgcctgtc accgcctcca cctgaggggc gaaatcggcg taatgttgag tggaagccca
     4201 aatcataaaa atcaaatgct gcggatcaat cggcgcgagt ttgccgcttt tgacccaacc
     4261 ggcaatcagc gccgatttct catcaattaa tgacttcaaa tcgcccgtca gttcatccat
     4321 taacagcggc gcgcctgcca gcatctccat acagaacagg cgcgaagcct gcggataatc
     4381 gcgtgagact tccagcttca gacggatgta ctctttgatc gccgccagcg gggcgaaatc
     4441 ttcacgaaac gcttttaacg gtgccagcca gatatcgaga atctgccgca gcacggcaat
     4501 atacagcgcc tcttttgacg gaaagtaata cagcagattg gtttttgaaa cacccgccaa
     4561 ctctgcaatt tgctccagcc gtgtgccgtg aaaaccgaat tgtgaaaaag tgtccagtgc
     4621 tgcgctaaga atcgcttttt tcttcgcgct tactgcgcgc gaacgtttac ccgttgtttt
     4681 cactgcgcct tgcgtcatgc gctctcccct cttgtagatt ggcaacagga tagcaaaacc
     4741 cccgcccggc ctcgaccttc tgcactctca tcgcgcacag atgcatgttt tatgtgcaac
     4801 tgttttgacc gtttagtcca ctttttacca gacattttag ataacgcgat gttaacacat
     4861 taataacact ccctttttaa aaactggcat ccgctttgca aacaagccaa tacgcggctg
     4921 aaaaggatat gcagaatgca gcgccccgtc tgactttcac tttacgagac gaagagaggt
     4981 tgatgatgaa aattggcgta ttcgtaccta ttggcaacaa cggctggctc atttcgaccc
     5041 acgcgccgca gtacatgccg acctttgaac tgaataaagc catcgtgcaa aaagcggagc
     5101 actaccattt cgatttcgcc ctgtcgatga tcaaactgcg tggctttggc ggcaaaactg
     5161 agttctggga tcacaacctt gagtcgttca ccttgatggc ggggctggcg gccgtgacct
     5221 cgcgcattca gatttacgcc accgccgcca ccttaacgtt acctccggca atcgtcgccc
     5281 gtatggccgc aaccatcgac tccatctctg gcgggcgttt tggcgtcaac ctcgtgactg
     5341 gctggcaaaa gcccgagtat gagcagatgg gtatctggcc tggcgatgac tatttctccc
     5401 gtcgttacga ctatctcacc gagtatgttc aggtgctgcg cgacctgtgg ggcacgggaa
     5461 aaagcgattt taaaggcgat tttttcacca tgaatgattg tcgcgtcagt ccgcaaccga
     5521 gtgtccccat gaaagtgatc tgcgccgggc aaagcgacgc tggcatggcg ttctccgctc
     5581 agtatgccga tttcaacttc tgtttcggca aaggcgtaaa tacacccacg gctttcgccc
     5641 cgaccgctgc gcggatgaaa caggccgcag agcaaaccgg gcgcgacgtt ggctcttatg
     5701 tattgtttat ggtgattgcc gatgaaaccg acgatgccgc tcgcgccaaa tgggaacact
     5761 acaaagcggg cgcggatgaa gaggcgttaa gctggctaac cgaacaaagt cagaaagata
     5821 cccgctccgg tactgacacc aacgtccgtc agatggccga tcccacttcg gcggtaaaca
     5881 tcaatatggg gacgttagtc ggttcttacg ccagtgtcgc gcgcatgtta gatgaagtcg
     5941 caagcgtgcc tggtgccgaa ggcgtgctgt taaccttcga cgattttctg tcgggaatcg
     6001 aaaccttcgg cgagcgcatt caaccactga tgcagtgccg cgcccatctc cctgtgctga
     6061 ctcaggaggt ggcatgatga cgacattaac cgctcgaccg gaagccatta ccttcgatcc
     6121 gcagcaaagt gcgctgatcg tggtggatat gcaaaacgcc tatgccacgc caggcggcta
     6181 cttagatctc gccgggtttg atgtctcaac cactcgcccg gtcattgcca acattcaaac
     6241 cgccgtgacc gcagcgcgag cggcagggat gttgatcatc tggtttcaaa atggctggga
     6301 tgaacagtat gtcgaggctg gcggccctgg ctcaccgaat tttcataaat cgaacgccct
     6361 caaaaccatg cgtaaacagc cgcagctgca ggggaaattg ctggcgaaag gctcctggga
     6421 ttatcaactg gtggatgaac tggtgccgca gcctggcgat attgtgctgc cgaagccgcg
     6481 ctacagcggt ttcttcaata cgccgctgga cagcattttg cgcagccgcg gaatacgcca
     6541 tctggttttc accagcatcg ctaccaacgt ctgcgtcgaa tcgacgctac gcgatggctt
     6601 ttttctggag tatttcggcg tggtgctgga agacgcaaca caccaggcgg ggccggaatt
     6661 tgtacagaaa gccgcgttgt tcaatatcga aacctttttt ggctgggtca gcgacgtcga
     6721 aacgttctgc gacgcgcttt ctcccacgtc ctttgctcgt atcgcttaag gagtttaacg
     6781 atgccaaaat ccgtaattat tcccgctggc agcagcgcac cgctggcccc cttcgttccc
     6841 ggcacgctgg ctgatggcgt ggtgtatgtc tccggtacgc tggcttttga tcaacacaat
     6901 aacgtgctgt ttgccgatga cccaaaggcg caaacccgcc acgttctgga aactatccgc
     6961 aaggtaatcg agacagcggg tggcacgatg gcggatgtga ccttcaacag catctttatt
     7021 accgactgga aaaattacgg cgcgattctg cattcagtgc ggactggtaa aacctgacgc
     7081 gctggtggaa atcgccacaa ttgcgcatat cgccaagtga ggccgcgatg aaactttcac
     7141 tctcacctcc cccttatgct gatgcgcccg tagtggtgtt gatttcgggt cttggcggta
     7201 gcggcagtta ctggttaccg caactggcgg tgctggagca ggagtatcag gtagtctgtt
     7261 acgaccagcg cggcaccggc aataatcccg acacgctggc agaagattac agtatcaccc
     7321 agatggcagc ggaactgcat caggcgctgg tagccgcagg gattgagcat tacgcagtgg
     7381 tcggccatgc gctcggtgcg ctggtgggaa tgcagctggc gctggatcat cccgcgtcgg
     7441 taactgtgct ggtctgcgtt aacggctggc tacgaataaa cgcccatacg cgccgctgtt
     7501 ttcaggttcg cgaacgatta ctgtatagcg gtggcgcgca ggcatgggtg gaagcgcagc
     7561 cgttgttcct ctatcccgcc gactggatgg cggcccgcgc acctcgcctt gaggcagaag
     7621 acgcgctggc actggcgcat tttcagggca aaaataattt actgcgtcga cttaacgccc
     7681 tcaaacgcgc tgactttagt caccatgcgg atcgcatccg ctgcccggtg caaatcatct
     7741 gcgccagtga tgatctgctg gtgccatcag catgttccag tgaacttcat gccgccctgc
     7801 ccgatagcca gaaaatggtg atgcgctatg gcggacacgc ctgcaacgtg accgatcccg
     7861 aaacgtttaa tgctctgtta ctcaacgggc ttgccagcct gttacatcac cgtgaagccg
     7921 ccctgtaagg aattgctatg aacgaagccg ttagcccagg tgcgcttagc accctgttca
     7981 ccgatgcccg cactcacaac ggctggcggg agacacccgt cagcgatgag acgttacggg
     8041 agctttatgc cctgatgaaa tgggggccga catcagctaa ctgttctccg gcacgtatcg
     8101 tgtttatccg cactgcagag ggaaaagaac gtctgcgccc ggcgctttcc agcggcaatc
     8161 tgcaaaaaac cctgaccgcg cccgtcaccg ctatcgtcgc atgggacagt gaattttatg
     8221 aacggttacc gcaactgttt cctcacggcg atgcccgcag ttggtttacc tccagcccac
     8281 aacttgccga agaaacagcg tttcgcaaca gttccatgca ggcggcttat ctgatcatcg
     8341 cctgccgtgc gctggggctg gataccggcc cgatgtcggg ttttgatcgt caacacgtag
     8401 acgacgcttt ttttgcaggc agcacgctga agagcaatct gctgattaat atcggctatg
     8461 gcgatagcag caaacttttt gcgcgcctgc cacgtctgtc ctttgaagaa gcctgcgggc
     8521 tgttgtaagg agcaaactat gaacattgtc gatcaacaaa cttttcgcga tgcgatgtcc
     8581 tgcatgggcg cggcggtcaa tatcatcacc acggacggtc cagccgggcg cgccgggttc
     8641 accgccagcg ccgtctgcag cgtgaccgac acgcctccca cattactggt gtgcctgaat
     8701 cgtggggcgt ccgtctggcc ggtattcaat gaaaatcgaa cgctgtgtgt aaatacactt
     8761 agcgccgggc aggagccgct ttcaaacctt tttggcggca aaacgcccat ggaacaccgc
     8821 tttgccgccg cccgctggca gaatggtgtg accggatgcc cgcaactgga agaggcgctg
     8881 gtttcgtttg actgccgtat cagccaggtg gtgagcgtcg gcacccacga cattttgttt
     8941 tgcgccatcg aagcgattca tcgtcacgcc accccctacg ggctggtgtg gttcgatcgc
     9001 agttatcacg cgctgatgcg ccctgcttgt taacctcctt aaggagacag ctgatggcaa
     9061 tgttcggtct tcctcactgg cagttaaaat cgacctctac agagagcggc gtggtcgcgc
     9121 cggatgaacg attgccgttt gctcagacgg cagtaatggg cgttcaacac gcggtggcga
     9181 tgtttggcgc gacggtgtta atgccgattc tgatggggct ggatcccaat ctttccattt
     9241 taatgtcagg gatcggcacg ttactgtttt tctttattac cggcgggcgc gtgcccagtt
     9301 atctcggctc cagcgcggct tttgtcggcg tggtgatcgc cgcaacgggc tttaacggtc
     9361 agggcattaa cccgaatatc agcattgcgc tgggcgggat tatcgcctgt gggctggttt
     9421 ataccgtcat tggcctggtg gtgatgaaaa tcggtacgcg ctggattgaa cggctaatgc
     9481 cgccggtggt gacgggtgcg gtggtgatgg cgattggcct gaacttagcg cctatcgcgg
     9541 taaaaagtgt atcggcttca ggctttgata gctggatggc ggtaatgacg gtgctgtgta
     9601 ttgggctggt ggcggtgttt acccgtggca tgatccagcg gttattgatt cttgtaggat
     9661 taattgtcgc ctgtctgctg tatggcgtga tgaccaacgt tttagggctg ggaaaagccg
     9721 tcgattttac tctcgtcagc catgctgcct ggttcggtct gccacatttt tccactcctg
     9781 cttttaatgg tcaggcaatg atgctgattg cgcccgtggc ggtgattctg gtggcggaaa
     9841 acttaggtca tctcaaggcg gtcgccggga tgaccggacg caatatggac ccgtatatgg
     9901 ggcgagcatt cgtaggcgat ggtctggcaa cgatgctttc tggttctgtc ggcagtagcg
     9961 gcgtcaccac ctatgctgaa aatatcggtg tgatggccgt gacaaaagtc tactcgacgc
    10021 tggtgtttgt tgctgctgcc gtcatcgcca tgctgttggg cttttcaccg aaatttggcg
    10081 cgctgattca taccattccg gccgcggtga ttggtggtgc atcaattgtt gtgttcgggc
    10141 tgattgccgt cgcaggcgca aggatatggg tacaaaaccg tgtcgattta agccagaacg
    10201 gtaatttgat tatggtcgca gtaaccttag tgctgggcgc gggtgatttt gctctcacgc
    10261 tgggcggttt tacgttggga ggaatcggta cagcaacctt tggcgcgatt ttactcaatg
    10321 cgttattaag cagaaggttg gttgacgttc caccgccaga agttgttcat caggagccat
    10381 aaacagaaaa ccgccggggt taccggcggt gatggctttt tgacgagctt aagaaaaaaa
    10441 tgcattcgtc attgattagc ccggctagtt gtcgcttttg ccgtgactgc tcttgccacc
    10501 ttttttacct gcttctgatg ctctttcgcg gtcttctgca aaattgccgg aaccgcctcg
    10561 atggtttgcc attactgacc tccgtctggg tatagacatc atattgctag aattaacgcg
    10621 gcgtatggcg gctgccatat tttcgctacg tgggactaag cttagtgaac tgacgtgttt
    10681 ctacccgcgt ttggtaatat cctgcaacag cttcttagcg agaatagata tcatctccaa
    10741 taattatcca taagccgctc ttatgattaa aaaaacgcga tgagataaaa atgtgtcatt
    10801 ttgcgacaaa attgcgtgct tgtgaaagtt gttataaatc aaataagtgg ttgtgaaatt
    10861 tgcactctga aaaggacgtc ttatctttaa ataagtggta gcgaatcgct acggaataga
    10921 gataacacga ggagtggtta gaaatggcta aagttctggt gctttattat tccatgtacg
    10981 gacatattga aacgatggca cgcgcagtcg ctgagggtgc aagcaaagtg gatggcgctg
    11041 aagttgtcgt taagcgtgta ccggaaacca tgtcgccgca attatttgaa aaagcaggcg
    11101 gtaaaacgca aactgcaccg gttgcaaccc cgcaagaact ggccaattac gacgccatta
    11161 tttttggtac acctacccgc tttggcaaca tgtccggtca aatgcgtacc ttcctcgacc
    11221 agacgggcgg cctgtgggct tccggcgcac tatacggaaa actggcgagc gtctttagtt
    11281 ccaccggtac tggcggcggt caggaacaaa ccatcacatc cacctggacg acccttgcgc
    11341 atcacggcat ggtgattgtc cccattggct acgcagcgca ggaattattt gacgtttcac
    11401 aggttcgcgg cggtacgccg tacggcgcaa ccaccatcgc aggcggtgac ggttcacgcc
    11461 agcccagcca ggaagaactg tctattgctc gttatcaagg ggaatatgtc gcaggtctgg
    11521 cagttaaact taatggctaa tcttcaacag gaggatacgc atgccaactc aagaagcgaa
    11581 agctcatcac gtcggtgaat gggcatctct gcgcaatacg tcgccggaaa tagccgaggc
    11641 cattttcgaa gtcgccgggt acgacgaaaa aatggcggaa aagatttggg aagaaggtag
    11701 cgatgaagtg ttagtcaaag cctttgctaa gaccgacaaa gattcgcttt tttggggcga
    11761 acagaccatc gaacgtaaaa acgtttaacc agcgactccc ccgtagcgaa cgggggagtt
    11821 ttctgttatt tcaccgcttc attcaacacg ctatcaaact tatccatcgg gcagaaacca
    11881 tcagcgtcta tcgggcaacc gcttaattcc agcgtcacgc gctgcgcagg tgcctgcagg
    11941 gttaacgcat cggcattacg taactgttcc gcactctggt acacatattc aattttcatc
    12001 aaatcgcgat tggctttgct gtcacgccaa cgctggaaaa cgattttgcc gccaatcggc
    12061 gtgcgttcgt tctggtcatg caactgatac ggtttgaaat ccagcgccgt taacagagag
    12121 gcaatgttgg agtcatgccc aactaacacc gtgatttttg gagcactggt gcgatcggtg
    12181 accagggctt tgtcgatata actgaccagc ggtttcgcaa cattgcgcgc cacttccggt
    12241 gaggtaaaca ggctgtcctg gtaaccgttt ttcagcttcg acaacacctt ccactgctgg
    12301 tcagatttga tttctcccca ggccacctga tccatcggaa aaccttcgta gtattgcaaa
    12361 gtaaacgcat ctaccagcga gttgccgact ttcagcggcc cggaaacacc tggttcttgt
    12421 tgatacttgg cgctaaaggt atttttgcca tccaccagcg aacactgttg tttctcttta
    12481 caggcagggg aatctttata gttaacgatt ttttccagta gctggtagct gtcggtaagc
    12541 tggagtttgc tgagctcttt ctccattgcc gccaccgcct gttcactgaa tgcggcggaa
    12601 tcatcggtga tcaccgggtt aaaggttggg tccatagtgc ccattttttc ctggtgatgc
    12661 acaggaatat cacaccccgg gaatgcgccg gtaataaaga actgtgcggt cgcaacggta
    12721 cgttgcagac tattggcata ggcataaacg gtgtccggcg gcgggcattc ccccgatttt
    12781 accatcccct gctctgccag ccattcacgc atgtaatggc ccatatacac ttcgagtacg
    12841 ccacctttgg tggtgagttg cccaccgggg acgtcccatt cgggccattt attcggcgtc
    12901 gactgctcca gcacactgcc attgttcgcc agcggcgcac gtaagttatg gcggctcatc
    12961 atgagcactt gctgtagctg atagccttcc ggtacggttt gtgcctgagc gtttgaagcg
    13021 agtaaaacta cccctgccac agctgcggcg attagcgttt tgttcattcc tggcacctct
    13081 tttgttatca atcactaaag agtgtgacag aaatatgact tttttccggg aacaggttcg
    13141 caaaatggct tgttgaacgg ctgtttttat attcatcttt aaaaacaggc aaggtttgac
    13201 gcaaactatg cctgattagc ccctgcctcg cgtacgccgc tgatgattgc acctgtggct
    13261 aaacagaata tcggcggcgt taccaataca gaggcaatat ttaccaataa aaaagtgtga
    13321 gactaataac aatggttttt agcatatgcc tctgtcatta aattgcataa cgagttaaaa
    13381 aacc
//
LOCUS       DBFIFI010000113        13350 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.113, whole genome shotgun sequence.
ACCESSION   DBFIFI010000113 DBFIFI010000000
VERSION     DBFIFI010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13350
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.113"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(168..1526)
                     /gene="plaP"
                     /locus_tag="N7B39_003107"
     CDS             complement(168..1526)
                     /gene="plaP"
                     /locus_tag="N7B39_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HGU2423046.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(1516..1578)
                     /gene="yoeI"
                     /locus_tag="N7B39_003108"
     CDS             complement(1516..1578)
                     /gene="yoeI"
                     /locus_tag="N7B39_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HGU2423047.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(1793..2722)
                     /gene="yeeY"
                     /locus_tag="N7B39_003109"
     CDS             complement(1793..2722)
                     /gene="yeeY"
                     /locus_tag="N7B39_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGU2423048.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHNVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(2768..3592)
                     /gene="yeeZ"
                     /locus_tag="N7B39_003110"
     CDS             complement(2768..3592)
                     /gene="yeeZ"
                     /locus_tag="N7B39_003110"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU2423049.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            4018..4068
                     /gene="hisL"
                     /locus_tag="N7B39_003111"
     CDS             4018..4068
                     /gene="hisL"
                     /locus_tag="N7B39_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HGU2423050.1"
                     /translation="MTRVQFKQHHHHHHPD"
     misc_feature    4044..4167
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            4214..5113
                     /gene="hisG"
                     /locus_tag="N7B39_003112"
     CDS             4214..5113
                     /gene="hisG"
                     /locus_tag="N7B39_003112"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HGU2423051.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDDAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTQRLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            5119..6423
                     /gene="hisD"
                     /locus_tag="N7B39_003113"
     CDS             5119..6423
                     /gene="hisD"
                     /locus_tag="N7B39_003113"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HGU2423052.1"
                     /translation="MSFNTIIDWNSCTAKQQRQLLMRPAISASESITRTVNDILDSVK
                     ARGDDALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MAHQVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKVGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            6420..7490
                     /gene="hisC"
                     /locus_tag="N7B39_003114"
     CDS             6420..7490
                     /gene="hisC"
                     /locus_tag="N7B39_003114"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HGU2423053.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIIAMRERVAQIIAER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            7490..8557
                     /gene="hisB"
                     /locus_tag="N7B39_003115"
     CDS             7490..8557
                     /gene="hisB"
                     /locus_tag="N7B39_003115"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HGU2423054.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPQLLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTRRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            8557..9147
                     /gene="hisH"
                     /locus_tag="N7B39_003116"
     CDS             8557..9147
                     /gene="hisH"
                     /locus_tag="N7B39_003116"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HGU2423055.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            9147..9884
                     /gene="hisA"
                     /locus_tag="N7B39_003117"
     CDS             9147..9884
                     /gene="hisA"
                     /locus_tag="N7B39_003117"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HGU2423056.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAISCWQNA"
     gene            9866..10642
                     /gene="hisF"
                     /locus_tag="N7B39_003118"
     CDS             9866..10642
                     /gene="hisF"
                     /locus_tag="N7B39_003118"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HGU2423057.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            10636..11247
                     /gene="hisIE"
                     /locus_tag="N7B39_003119"
     CDS             10636..11247
                     /gene="hisIE"
                     /locus_tag="N7B39_003119"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HGU2423058.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(11344..12321)
                     /gene="wzzB"
                     /locus_tag="N7B39_003120"
     CDS             complement(11344..12321)
                     /gene="wzzB"
                     /locus_tag="N7B39_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HGU2423059.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIESSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKELRIRQIQEALQYANQAQVAKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIENLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(12467..>13350)
                     /locus_tag="N7B39_003121"
     CDS             complement(12467..>13350)
                     /locus_tag="N7B39_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HGU2423060.1"
                     /translation="SVESVIKDVVEINPYAVMVIKSTVPVGFTEAMHKKYRTENIIFS
                     PEFLREGKALYDNLHPSRIVIGERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIK
                     LFANTYLAMRVAYFNELDSYAESLGLNSRQIIEGVCLDPRIGNHYNNPSFGYGGYCLP
                     KDTKQLLANYQSVPNNLISAIVDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNF
                     RASSIQGIMKRIKAKGVEVIIYEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEE
                     LRDVADKVYTRDLFGSD"
BASE COUNT         3387 a         3470 c         3405 g         3088 t
ORIGIN      
        1 ccagatgaat gttaatgggg aaattattca tgactggtgg aatgcagacc aataaccaaa
       61 ttctctaaat tagaacaaat ggttattaat gaggatttcg ataaaaaaga ataaaaaaac
      121 cggagccaaa tgttcattcg actccggttt attattagaa atgatgatta tgctacgtct
      181 tcgtactgcg gaaccgggtt acggaagctc ttggtgacgc aagccaggta aatcagaccg
      241 atagcagccc agatcagacc cagaaccatt gagctttctt ccaggttaac ccacagcgca
      301 ccaacggtca gcgcaccaca catcggcagg aacagatact ggaagtgatc tttcagcgtc
      361 ttattacgct tctcacggat ccagaactgc gagatgaccg acaggttaac gaaggtgaac
      421 gccaccaacg caccaaagtt gatcagcgcc gtagccatta ccaggtcgaa gttgattgcc
      481 agcagggcaa tcgcgccaac caggatgatg ttcatcgccg gagtacgcca tttcgggtgt
      541 acataaccga agaaactttt cgggaacacg ccgtcacgac ccattacgta catcagacgc
      601 gctacgcctg cgtgcgccgc cataccggac gccagtacgg taatggtgga gaagatcagc
      661 gcaccgacct ggaacgcttt acccgcaacg tacagcatga tttcaggctg tgacgcatcc
      721 ggatctttaa agcgagagat atccgggaag tacagctgca ggaagtaagt tgcaaagatg
      781 aagatcatgc cgccgatcag cgcggtcagg aaaatcgcac gcgggatcac gcgctctgca
      841 tctttggttt cttccgacag attgctgatg ccgtcaaagc cggtaaagga gaagcacagg
      901 attgtcgccc cggtaatcat cgggataaca tgcgcatcgc cagaccagaa tggacgagtg
      961 ctcgccagcg taccagcgcc ttcaccttca aatacgccat aaacaaccat gcccagaatc
     1021 accgcgatca gcactacctg caacacgacg attacagtgt tgaagttcgc tacggatttc
     1081 agactacgca ggttaaaggc ggtcataaag gccaccagcg ccaccacaaa catccacgat
     1141 gggatggaag gcaccagagc ttcaaaatag attttcgcca gcagaatgtt gatcatcggc
     1201 gcgaacagat agtcgagcag agaagaccaa cccaccataa agccgacagt cgggctaatg
     1261 gatttctggg cgtaagtata tgcagagcca gcagaaggat agcggcgaac cagcttcccg
     1321 tagctcagag ccgtaaacag gatcgcaatc aatgcgaacg cataggctgt cggcacatga
     1381 ccatccgtga ggcctgaaac gataccaaaa gtatcaaaca gcgtcatcgg ctgcatatag
     1441 gcaagaccca tcattacaac cggaactaac gtaagcgttt tacgcaattc cacgcgagag
     1501 gtgtttggag taacgttatg cgacatggtc attctccttt acggtgataa ccgtcgcgta
     1561 agcaaaaaat tgccccattt ttttggattc ctcagcgaca acaactgtcg atttttagta
     1621 aatatctatc cggtacgaag cccggcctct tggtatgaat gattggtttg aagcaaaaaa
     1681 taaccgacgc tgatgaaacg tcggttttta gtcatttttt gacagcggcg cattgtgcct
     1741 aaatgggggg gaaatgacaa gagaatgaga ggcttgtcat aataattttt ctttaaatgg
     1801 ctgattttcc gtcatcagat tttgcataaa caccggcgat cgcactattt gctaaaattt
     1861 catcccgcca ccagccgctg gcaagtcctg cggtctgttc attccagcca atccagacgg
     1921 gttcaaagag agtttgggcg accactttct tctcgattaa cgcgccacta tcgagaaaac
     1981 gttgcgccag ataacggggt aaatagccgc agccgaggcc gctaatttgc agctccagct
     2041 tggttttaaa atcgaaaacg gtaatggctt cttgttcgtc gagaagttgt gaggccgtag
     2101 aagcgcctgc ctgcgcagtg tcccccacca caatcgcccg atatcgttta atgatacgac
     2161 ggtttaatgg ttcttcttcc tgggctaacg gatgatgagg cgcgaccgca aagacttgtt
     2221 ccagatcgcc caaccgcgag aaaccaaact cactggaaga tggtggctca tgcatcgcgc
     2281 caacaataat atccgctcgc ccctgagtca gggcatccca tgaaccaccg agtacgccat
     2341 tgataaactt caggcgcgtc acgttatgat gttgataaaa agcttcaatg agtggcgcaa
     2401 gcagagaaaa aggaaacgtg tcgtccacgc caatcaccag ctcgttttcc cagccttcat
     2461 gcagtttgat cgcctgcttt tccagttctc gcacggtatg cagaacttcc cgccccttct
     2521 ctaataacat ttttccggta cgggtgaatt tagcgcggtg accgctacga tcaagcaatt
     2581 gaatattaag gtcgctttcc agcttgtgaa cggtgtaact cagagcggac ggcgtcttgt
     2641 agagtttagc cgacgccgcc gcaaaactgc cttctttttc taaggcatcg aggatcatca
     2701 acacatccag cagtggtttc atacacgccc ccttgcagtg catgcggcgt cctgctggcg
     2761 tgatcgttta ctccagcggc attaccagcg gatcgggata ctggtattca aatcccagtt
     2821 cattacaaat ccgactgcca tcaataatct tacctttgcc gctgtccaga ctatttctga
     2881 attgcggagg ttccagcccc agtaaacggg ccatctgcgg atagaaaaca ttacgcgcag
     2941 ggtgagctgg cgcacatata ttatagatgt gtccgccttt aggtgcctgt aacaacagag
     3001 tgatagcgcc aataacatct tctaaatgga ctaaattaac accatgttca ccatcaggcg
     3061 cggtttttcc ggcaaagaag cgtccgggat gacgccccgg tccgaccagg cccgcaagac
     3121 gcagaatatc gaccgaagta ccgggtaaat tgtgcagcca gtcttcgagt tcttctaata
     3181 ctcgtccact gttggtcacc ggattacgcg gggtggtttc tttcaccgtg ccttgcgcgt
     3241 cgccatagac agacgttgaa ctggtaaaaa taattcgggg aatacgatgg gccagcgcgc
     3301 tatccactaa ctcttgtacc gcttgtaaat agaactcatc gccggggccg ctacgacggg
     3361 ccggaagcgt aatgaccagc gcatcggcat ccatcagggc atccagatcg tcagaatcgc
     3421 aaactaactc aggctccatg cgaagcagat agctatcaat gccactcatt cgggccgctt
     3481 cgacaccatc ttgtgtggtt ttactcccgg tgacttgcca gcctcgcgct gaaagtgaca
     3541 tcgccagcgg catgcctaac caccctaacc cgacaattgc gacctttttc atccgttatc
     3601 tcctgataca ccttagatct ataaggctac gctagcgtat caaaactgac aattcattct
     3661 atgaatgaaa tcgaaactgt tagcaaatat accattgcct gatgcgctcc gcttattagt
     3721 cctacaaaac atcaatatat tgaatttagg caacatttgt agaccagata aggcattcac
     3781 gccgcatccg gcaggaacaa agcccacttt agttacagaa cacaatgttc ttgagggttt
     3841 taatgacata ttttcacgat tactttttta acctagtcac tcttagcccc ctcctttccc
     3901 cgctcattca ttaaacaaat ccatcgccat aaaatatata aaaaagccct tgctttctaa
     3961 cgtgaaagtg gtttaggtta aaagacatca gttgaataaa cattcacaga gacttttatg
     4021 acacgcgttc aatttaaaca gcaccatcat caccatcatc ctgactagtc tttcaggcga
     4081 tgtgtgctgg aagacattca gatcttccag tggtgcatga acgcatgaga aagcccccgg
     4141 aagatcacct tccgggggct tttttattgc gcggttgata acggttcaga caggtttaaa
     4201 gaggaataag aaaatgacag acaacactcg tttacgcata gctatgcaga aatccggccg
     4261 tttaagtgat gactctcgcg aattgctggc gcgctgtggc attaaaatca atcttcacac
     4321 ccagcgcctg atcgcgatgg cagaaaacat gccgattgat attctgcgcg tgcgtgacga
     4381 cgacattccc ggtctggtaa tggacggcgt ggtagatctt gggattatcg gcgaaaacgt
     4441 gctggaagaa gagctgctta accgccgcgc ccagggtgaa gatccacgct actttaccct
     4501 gcgtcgtctg gatttcggcg gctgccgtct ttcgctggca acgccggttg atgatgcctg
     4561 ggacggcccg ctctccttaa acggtaaacg tatcgccacc tcttatcctc acctgctcaa
     4621 gcgttatctc gaccagaaag gcatctcttt taaatcctgc ttactgaacg gttctgttga
     4681 agtcgccccg cgtgccggac tggcggatgc gatttgcgat ctggtttcca ccggtgccac
     4741 gctggaagca aacggcctgc gcgaagtcga agttatctat cgctcgaaag cctgcctgat
     4801 acaacgcgat ggcgaaatgg aagaatccaa acagcaactg atcgacaagc tgctgacccg
     4861 tattcagggt gtgatccagg cgcgcgaatc aaaatacatc atgatgcacg caccgaccca
     4921 acgtctggat gaagtcatcg ccctgctgcc aggtgccgaa cgcccaacca ttctgccgct
     4981 ggcgggtgat caacagcgcg tagcgatgca catggtcagt agcgaaaccc tgttctggga
     5041 aacgatggaa aaactgaaag cgctgggtgc cagttcaatc ctggtcctgc cgattgagaa
     5101 gatgatggag tgatcgccat gagctttaac acaatcattg actggaatag ctgtactgca
     5161 aagcaacaac gccagctgtt aatgcgcccg gcgatctccg cttctgaaag cattacccgc
     5221 actgttaacg atattctcga tagcgtgaaa gcacgcggtg atgacgccct gcgggaatat
     5281 agcgcgaagt ttgataaaac cacggttacc gcactgaagg tgtctgctga ggaaattgcc
     5341 gccgccagcg aacgcctgag cgacgagcta aaacaggcga tggcggtggc agtaaagaat
     5401 attgaaacct tccacactgc gcaaaaactg ccgccggtag atgtagaaac gcagccaggc
     5461 gtgcgttgcc agcaagtcac gcgcccggta gcttcagttg ggttgtatat tcctggcggc
     5521 tccgccccgc tcttctcaac ggtattaatg ctggcaactc cggcgcgtat tgcgggctgt
     5581 aaaaaagtgg tgttgtgctc accgccgccg attgccgatg agatccttta tgcggcgcag
     5641 ctgtgcggtg tgcaggacgt gtttaacgtc ggcggcgcac aggccattgc cgcgctggcg
     5701 tttggtacgg aatctgtgcc gaaagtggac aaaatcttcg ggccgggtaa cgcctttgtc
     5761 accgaggcaa aacgtcaggt gagccagcgt ctggacggtg cggcgatcga tatgcccgca
     5821 ggcccgtcgg aagtgttggt cattgccgac agcggcgcaa cgccggattt cgtggcttct
     5881 gatttgcttt ctcaggctga acacggcccg gactcacagg tgattttact gacgcctgac
     5941 gctgatatgg cgcatcaagt tgccgaagcc gtcgaacgcc agttagcaga actgccgcgt
     6001 gccgaaaccg cccgccaggc actgaacgcc agccgcctga tcgtgactaa agatttagcg
     6061 cagtgcgtgg agatctccaa ccagtacggc ccggagcacc tgatcattca gacccgcaac
     6121 gcccgcgaac tggtcgatgg catcaccagc gccggttcgg tatttcttgg tgactggtca
     6181 ccggaatcgg caggtgatta cgcctccgga accaaccatg ttctaccgac ttacggctac
     6241 accgccacct gttccagcct cggactggcg gatttccaga agcggatgac cgtgcaggaa
     6301 ctgtcgaaag taggtttctc cgcgctggct tcgaccattg aaacactggc cgccgccgag
     6361 cgcctgaccg cccacaaaaa tgccgttact ttgcgtgtta acgcccttaa ggagcaagca
     6421 tgagcaccgt gactattacc gatttagcgc gtgaaaacgt ccgcaacctg acgccgtatc
     6481 agtcggcgcg tcgtctgggc ggtaatggcg acgtctggct gaacgccaac gaatatccca
     6541 cagccgtgga gtttcagctt actcagcaaa cgctcaaccg ctacccggaa tgccagccga
     6601 aagcggtgat tgaaaattac gcgcaatatg caggcgtaaa accggaacag gtgctggtca
     6661 gccgtggcgc ggacgaaggt attgaactac tgattcgcgc tttttgcgaa ccgggtaaag
     6721 acgccatcct ctactgcccg ccaacgtacg gcatgtacag cgtcagcgct gaaaccattg
     6781 gcgtcgagtg ccgcacagtg ccgacgctgg aaaactggca actggactta cagggcattt
     6841 ccgacaagct ggacggcgta aaagtggtct atgtttgcag ccccaacaac ccaaccgggc
     6901 aactgatcaa tccgcaggat tttcgcaccc tgctggagtt aacgcgcggt aaggcgattg
     6961 tggttgccga tgaagcctat atcgagtttt gcccgcaggc atcgctggct ggctggctgg
     7021 cggaatatcc gcacctggct attttgcgca cactgtcgaa agcttttgct ctggcgggcc
     7081 ttcgttgcgg atttacgctg gcaaacgaag aagtcatcaa cctgctgatg aaagtgattg
     7141 ccccctaccc gctctcgacg ccggttgccg acattgcggc ccaggcgtta agcccgcagg
     7201 gaattatcgc catgcgcgaa cgggtggcgc aaattattgc agaacgcgaa tacctgattg
     7261 ccgcactgaa agagatcccc tgcgtggagc aggtttttga ctctgaaacc aactacattc
     7321 tggcgcgctt taaagcctcc agtgcagtgt ttaaatcttt gtgggatcag ggcattatct
     7381 tacgtgatca gaataaacaa ccctctttaa gcggctgcct gcgaattacc gtcggaaccc
     7441 gtgaagaaag ccagcgcgtc attgacgcct tacgtgcgga gcaagtttga tgagtcagaa
     7501 gtatcttttt atcgatcgcg atggaaccct gattagcgaa ccgccgagtg attttcaggt
     7561 ggatcgtttt gacaaactcg cctttgaacc gggcgtgatc ccgcaactgc tgaagctgca
     7621 aaaagcgggc tacaaactgg tgatgatcac taatcaggat ggtctgggaa cacaaagttt
     7681 cccgcaggcg gatttcgatg gcccgcacaa cctgatgatg cagatattca cctcgcaagg
     7741 cgtacagttt gatgaagtgc tgatttgtcc gcacctgccc gccgatgagt gcgactgccg
     7801 taaaccgaaa gtaaaactgg tggagcgtta tctcgctgag caagcgatgg atcgcgccaa
     7861 cagttatgtg attggcgatc gcgcgaccga tattcaactc gctgaaaaca tgggcattaa
     7921 tggtttacgc tacgaccgcg aaaccctgaa ctggccaatg attggcgagc aactcaccag
     7981 acgtgaccgt tacgctcacg tagtgcgtaa taccaaagag acgcagattg acgttcaggt
     8041 gtggctggat cgtgaaggtg gcagcaagat taataccggc gttggcttct ttgatcacat
     8101 gctggatcag atcgccaccc acggcggttt ccgtatggaa atcaacgtca aaggcgacct
     8161 ctatatcgac gatcaccaca ccgtcgaaga taccggcctg gcgctgggcg aagcgttaaa
     8221 aattgccctc ggcgataaac gcggtatttg ccgctttggt tttgtgctgc cgatggacga
     8281 atgccttgcc cgctgcgcgc tggatatctc tggtcgcccg cacctggaat ataaagccga
     8341 gtttacctac caacgcgtgg gcgatctcag caccgagatg atcgagcact tcttccgttc
     8401 gctctcttac accatgggcg tgacgctcca cctgaaaacc aaaggtaaaa acgatcatca
     8461 ccgtgtagag agcctgttca aagcctttgg tcggaccctg cgccaggcca tccgcgtgga
     8521 aggcgatacc ctgccctcgt cgaaaggagt gctgtaatga acgtggtgat ccttgatact
     8581 ggctgcgcca acctgaactc ggtgaagtct gccattgcgc gtcacggtta tgaacccaaa
     8641 gtcagccgtg acccggacgt ggtgttgctg gccgataaac tatttttacc cggcgttggc
     8701 actgcacaag cagcgatgga tcaggtgcgt gagcgcgagc tgtttgatct catcaaagcc
     8761 tgtacccaac cggtgctggg catctgctta gggatgcaac tgctggggcg gcgcagtgaa
     8821 gagagcaacg gcgtcgattt gctgggcatc atcgacgaag acgtgccgaa aatgaccgac
     8881 tttggtctgc cgctgccgca tatgggctgg aatcgcgttt acccgcaggc aggcaaccgc
     8941 ctgtttcagg ggattgaaga cggcgcgtac ttttactttg ttcacagcta cgcaatgcca
     9001 gtcaatccgt ggaccatcgc ccagtgtaat tacggcgaac cattcaccgc ggcggtacaa
     9061 aaagataact tctacggcgt gcagttccat ccggagcgtt ctggtgccgc tggcgctaag
     9121 ttgctgaaaa acttcctgga gatgtgatga ttattccggc attagattta atcgacggca
     9181 ctgtggtgcg tctccatcag ggcgattacg gcaaacaacg cgattacggt aacgacccgc
     9241 tgccgcgctt acaggattac gccgcacaag gtgccgaagt gctgcatttg gtggatctga
     9301 ccggggcaaa agatccggca aaacgtcaaa tcccgctgat taaaaccctg gtcgctggcg
     9361 ttaacgttcc ggtgcaggtt ggcggcggcg tgcgtagcga agaagacgtg gcggcgttac
     9421 tggaagctgg ggttgcgcgc gtggtggttg gctccactgc ggtgaaatca ccagaaatgg
     9481 tgaaaggctg gtttgaacgt ttcggtgccg atgccttagt gctggcgctg gatgtccgta
     9541 ttgacgagca aggcaacaag caggtggcgg tcagcggctg gcaagagaat tcgggcgtgt
     9601 cactggaaca actggtggaa acctatctgc ccgtcggcct gaaacatgtg ctgtgtaccg
     9661 atatctcgcg cgacggcacg ctggcaggct ctaacgtctc tttatatgaa gaagtgtgcg
     9721 ccagatatcc gcaggtggca tttcagtcat ccggcggcat tggcgacatt aacgatgttg
     9781 ctgccctgcg tggcactggc gtgcgcggcg taatagttgg tcgtgcatta ctggaaggta
     9841 aattcaccgt gaaggaggcc atctcatgct ggcaaaacgc ataattccat gtctcgacgt
     9901 tcgtgatggt caggtggtaa aaggcgtaca gtttcgcaac catgaaatca ttggcgatat
     9961 cgtgccgctg gcaaaacgct acgccgaaga aggtgcagac gaactggtgt tctacgatat
    10021 caccgcttcc agcgatggcc gtgtggtaga taaaagttgg gtatctcgcg tggcggaagt
    10081 gatcgacatt ccgttctgcg tggcgggtgg gattaagtct ctggaagatg ccgcgaaaat
    10141 tctttccttt ggcgcggata aaatttccat caactcccct gcgctggcgg acccgacgtt
    10201 aattactcgc ctggcggatc gatttggcgt gcagtgtatt gtggtgggta ttgatacctg
    10261 gtacgacgct gaaaccggta aatatcatgt gaatcaatat accggcgatg aaagccgcac
    10321 ccgcgtcact cagtgggaaa cgctcgactg ggtacaggaa gtgcaaaaac gcggtgccgg
    10381 agaaatcgtc ctcaacatga tgaatcagga cggcgtgcgt aacggttacg acctcgaaca
    10441 actgaaaaaa gtgcgtgaag tttgccacgt cccgctgatt gcttccggtg gcgcgggcac
    10501 catggaacac ttcctcgaag ccttccgcga tgccgacgtt gacggcgcgc tggcagcttc
    10561 cgtattccac aaacaaataa tcaatattgg tgaattaaaa gcgtacctgg caacacaggg
    10621 cgtggagatc aggatatgtt aacagaacaa caacgtcgcg aactggactg ggaaaaaacc
    10681 gacggactga tgccggtgat tgtgcaacac gcggtatccg gcgaagtgtt aatgctgggc
    10741 tatatgaacc cggaagcctt agacaaaacc atcgaaagcg gcaaagtcac cttcttctcg
    10801 cgcactaaac agcgactgtg gaccaaaggc gaaacgtcgg gcaatttcct caacgtagta
    10861 aatattgcgc cggactgcga caacgacacg ctactggtac tggcgaatcc tatcggacca
    10921 acctgccaca aaggcaccag cagctgcttc ggcgacaccg ctcaccagtg gctgttcctg
    10981 tatcaactgg aacaattgct cgccgaacgc aaatctgccg acccggaaac ctcctacacc
    11041 gccaaactgt atgccagcgg caccaaacgc attgcgcaga aagtgggcga agaaggcgtg
    11101 gaaaccgcac tggccgcgac tgtacatgac cgctttgagc tgaccaacga ggcgtctgac
    11161 ttgatgtatc acctgctggt attgttgcag gatcaggatc tggatttaac aacggtgatt
    11221 gagaacctgc gtaaacgtca tcagtgagtt gcggggtaag cggatgcgat attgttgtcg
    11281 catccggcaa aaaaaacggg caaggtgtca ccaccctgcc ctttttcttt aaaaccgaaa
    11341 agattacttc gcgttgtaat tacgcagagc gttacgcccc agcacaatcc ccgcgccaac
    11401 catgccgccc agcagcaccg ccagaatcaa ggtgattgcc tttttcggac tatcgcgacg
    11461 aataggtaac gtcggtttca ttacatagcg gtaagcatga atatcaagat catcaacctt
    11521 taagttttca atatccaaca ggttttgacg tgtctgatag tagtttggtg agaacaccaa
    11581 cggacgggtc gcctcatgct taatcatcga ctccagcgct tcgctcccca aaaggaacaa
    11641 cgtatcttgc gtcacatctt cagtctgctg aacctgtggc tttgccacct gcgcctgatt
    11701 cgcatactgc aacgcttcct gaatctgacg gatacgcagc tctttctgct cctgcgcaac
    11761 tacttcctgc gttcttaaag agtcctgcaa gtttttccgt cccagagcaa tgttgtcttt
    11821 gagatccttt tctagctctt gattcacttt atcatcaact tgctgaatgt attgggccaa
    11881 cttcatttgt gctccctctg cagtttgccc aacataagaa acagtcaatg gtaattgctg
    11941 gttcttaaca gaagattcga tggtaagttt ttctggttct tcctgattat ccagcgtttc
    12001 tgctaatgct gagaaggcag aactgaagcg accaatcaac gtctcctgca aatccgatac
    12061 tttcggtgca gcctgaccat agataacatt catggcattg ttatagccag caatttgccc
    12121 cacgtcgggc tgagtgataa ttgctgttga cgtccatttc tccttcgcta ctgccaaata
    12181 accaatagcc agagcaatag ccacaatgac ggaaatgatg attgtcatct tgccacgcca
    12241 caactgcact agtaaatcaa tcaaatcaat ctgttccggg tcatggtttt gcccagaaac
    12301 attattattt tctactctca tcattaccct aactgacgga aatttccaag tccgcatagt
    12361 ttagcgataa tccctgtgaa tgctatagga taagtgataa aaataatgaa ttattgcgga
    12421 ggtaacaaaa gacaatcagg gcgtaaatag ccctgataac aagatgttag tcgctgccaa
    12481 agagatcgcg ggtgtagacc ttatctgcca catccctaag ctcttctgcc atacggttgg
    12541 aaataatgac gtcggcttgt tgtttgaagg tggcgagatc acgttccagg cgagagttga
    12601 agaatgagtc ttctttcatc actggctcgt agatgattac ttcaacacct ttcgccttga
    12661 tacgcttcat aatcccctga atagaagacg cacggaagtt atctgaaccg ctcttcataa
    12721 tcagacgata aatacctacc acttgcggct tgcgtgacaa aatggcatcg gcaataaaat
    12781 ctttacgcgt gcggttagca tcgacaattg ccgagatcag gttattcggc acagactggt
    12841 agttcgccag taactgcttg gtatctttcg gcaaacaata accaccataa ccaaacgacg
    12901 gattgttgta atggttgcca atacgtgggt cgagacaaac gccttcgatt atttgacggg
    12961 aattcagacc taaactttct gcgtagctat ccagttcgtt aaagtacgcc acgcgcatcg
    13021 cgaggtaggt gtttgcaaaa agtttaatcg cttctgcttc agtggagtcg gtaaacaggg
    13081 ttgggatatt ttgcttaatc gcgccttcct gtaataacgc agcaaaacgt tctgcgcgtt
    13141 ccgaacgctc accgattaca atacgtgacg gatgaaggtt atcgtaaaga gctttacctt
    13201 cgcggagaaa ttccggagag aaaataatat tttcagtacg atatttctta tgcatcgctt
    13261 cggtaaaacc aacgggaacc gttgatttga tgaccataac cgcataagga tttatctcaa
    13321 ctacgtcttt aattactgat tctacactgg
//
LOCUS       DBFIFI010000114        13330 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.114, whole genome shotgun sequence.
ACCESSION   DBFIFI010000114 DBFIFI010000000
VERSION     DBFIFI010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13330
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.114"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..80
                     /locus_tag="N7B39_003122"
     CDS             <1..80
                     /locus_tag="N7B39_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HGU2423061.1"
                     /translation="ILEIWNALQAGAPYEPLIAELTGGV"
     gene            complement(207..2126)
                     /locus_tag="N7B39_003123"
     CDS             complement(207..2126)
                     /locus_tag="N7B39_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6538 domain-containing protein"
                     /protein_id="HGU2423062.1"
                     /translation="MSQRYKLYRRTSGIYVVRISVPQRFRRYAGQCEIHTSTGTHDLH
                     EAKQKSALLLAVWYQTLQEYEQLDYRTLSDCAPLLAGEGMISLSNFAQSIELPISQLI
                     REVVNRNLPVFWLATGQFGFYVDEFNAVEREPGAKREKQSDDEKDQPKEVIILNSAFE
                     LGIESFANGYLRPFNPRHTLDCLLSAGVSEGEAAFRTSGDNQSGGWFFDLPGVDITAD
                     SLLISKVHAEGLRLTWLVKTTPPAVSIHPAVPLVAPVIANEYVHRKHYNENLSWLREE
                     YLKHRRKGKVSEAALRDIRYYFDLMIEVMGDIQLEDFDRDFLRAYESKLRTIPANRNL
                     MKGKHGVKTLDELIAKAAECGDKLMTEESVKKYINGLYGAMEWAVDDGKFLKSPCDNF
                     FPPDDKGEREQDHTDIFEPHEIKAIFSQPWFVAGTVERNAQGRFHQYCPFHYWAPLLG
                     LMTGARVNEIAQLMLDDVLADDGVYYLNLESDSENGKKLKNANSRRKIPVHSTLIELG
                     FIEYVDALKAAGYDRLFPELKPHKTKGYGRPVSAWFNESLLAGRLKLERDRSKSFHSF
                     RHSVSTLLKEKGVSSELRGQLLGHVRGKTETEVRYSKDLKPVHMVEVVEKIDFSLPEI
                     ARFNIPDGLDAVELI"
     gene            complement(2217..2301)
                     /locus_tag="N7B39_003124"
     tRNA            complement(2217..2301)
                     /locus_tag="N7B39_003124"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2265..2267),aa:Leu,seq:caa)
     gene            2497..3516
                     /gene="ahr"
                     /locus_tag="N7B39_003125"
     CDS             2497..3516
                     /gene="ahr"
                     /locus_tag="N7B39_003125"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HGU2423063.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELRPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLPVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIKHVRDGKARYRVV
                     LKADF"
     gene            3646..5148
                     /gene="yjgR"
                     /locus_tag="N7B39_003126"
     CDS             3646..5148
                     /gene="yjgR"
                     /locus_tag="N7B39_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HGU2423064.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIDVNDWQPHANPVVVWDIFG
                     EKGHSVRATVSDLGPLLLVRLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPAKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(5309..6391)
                     /gene="lptG"
                     /locus_tag="N7B39_003127"
     CDS             complement(5309..6391)
                     /gene="lptG"
                     /locus_tag="N7B39_003127"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HGU2423065.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(6391..7491)
                     /gene="lptF"
                     /locus_tag="N7B39_003128"
     CDS             complement(6391..7491)
                     /gene="lptF"
                     /locus_tag="N7B39_003128"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HGU2423066.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(7554..7604)
                     /gene="ytgA"
                     /locus_tag="N7B39_003129"
     CDS             complement(7554..7604)
                     /gene="ytgA"
                     /locus_tag="N7B39_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HGU2423067.1"
                     /translation="MSIIILATISTTQENK"
     gene            7758..9269
                     /gene="pepA"
                     /locus_tag="N7B39_003130"
     CDS             7758..9269
                     /gene="pepA"
                     /locus_tag="N7B39_003130"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HGU2423068.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            9623..10066
                     /gene="holC"
                     /locus_tag="N7B39_003131"
     CDS             9623..10066
                     /gene="holC"
                     /locus_tag="N7B39_003131"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HGU2423069.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            10066..12921
                     /gene="valS"
                     /locus_tag="N7B39_003132"
     CDS             10066..12921
                     /gene="valS"
                     /locus_tag="N7B39_003132"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HGU2423070.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(12976..>13330)
                     /locus_tag="N7B39_003133"
     CDS             complement(12976..>13330)
                     /locus_tag="N7B39_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HGU2423071.1"
                     /translation="AQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYH
                     GMLYRMCALVVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGF
                     LASISRGIMPSLPFL"
BASE COUNT         3337 a         3433 c         3536 g         3024 t
ORIGIN      
        1 aaattttaga aatctggaat gcactacaag ctggcgcacc ttatgaacca ctaatcgcag
       61 aattgactgg cggagtgtga ctaactaaat aattttcccc taaaaaaatt cgatgtagga
      121 aaatatatat aagtaagaat catgagtgtc cgctcctcgc tcaaggcgga ctgttagact
      181 tgattgtatg ctgtcagaga aaaccgtcag atcaattcta cagcatccag cccatcagga
      241 atgttgaatc tcgctatctc gggcaaagaa aaatcaatct tttcgacaac ctcaaccatg
      301 tgaaccggtt ttaaatcttt gctgtatcgc acttcagttt ctgttttgcc tcgcacatgc
      361 ccaagtagct gcccacgcag ttccgaacta acacccttct ctttaagcaa agttgaaaca
      421 gaatgccgga aagagtggaa agatttgctt ctgtctcttt caagttttaa tcgacccgca
      481 agcaatgatt cattgaacca tgcggaaacc ggcctaccat agcctttggt tttatgtggt
      541 ttaagctcgg gaaaaagacg gtcatacccg gcagctttca acgcatccac atactcgata
      601 aaaccgagtt caatcagcgt agaatgaacc ggaatcttgc ggcgggaatt ggcgtttttt
      661 agtttctttc cgttttcgct atcgctttca aggttcaggt aataaacgcc gtcatctgcc
      721 agaacatcgt ccagcattaa ctgtgcaatc tcgttaaccc ttgcccccgt catcaagccc
      781 aacaacggcg cccaatagtg aaacgggcaa tattgatgga atcgcccttg cgcattacgt
      841 tcaacagttc cagcgacaaa ccacggttgc gaaaaaattg ccttaatttc atgcggttca
      901 aatatgtcag tgtgatcctg ctctcgctca cctttgtcat cgggagggaa aaagttgtcg
      961 catggcgatt tcagaaactt accatcatca acagcccact ccattgcacc ataaaggccg
     1021 ttgatatact ttttgacaga ctcttctgtc atcagtttat cgccacattc tgccgctttg
     1081 gcgattaact catccagcgt cttaaccccg tgcttacctt tcatcaaatt acggttagca
     1141 ggaattgtgc gcaacttgct ctcataagcc cggaggaaat cacggtcgaa atcttccaac
     1201 tgaatatccc ccatcacttc aatcatcaaa tcgaagtaat agcgaatatc gcggagcgcc
     1261 gcttctgata ccttgccctt acgtcgatgt ttcaaatact cttcacgaag ccatgacaag
     1321 ttttcattgt aatgtttgcg gtgaacatat tcattagcga taacaggagc gacaagaggc
     1381 acggcagggt gaatgctaac tgctggtggc gtggtcttaa ccagccatgt aagtcgaagg
     1441 ccttcagcat gaactttgct aatcaacagg ctatcagcag ttatatctac gccgggtaaa
     1501 tcgaagaacc aacctccact ttggttatca ccactagttc gaaatgcagc ctctccttcg
     1561 gatactccag cgctcaacag acaatctaaa gtatgccggg gattgaaggg gcggagataa
     1621 ccatttgcga agctctcgat acccagctca aacgcgctat tgagaatgat gacttcttta
     1681 ggttgatcct tttcatcatc agactgtttt tctcgttttg cacccggttc ccgctctact
     1741 gcattaaatt catcaacata gaaaccgaac tgaccagtcg ccagccagaa taccgggagg
     1801 ttacgattaa ccacctctcg aatcaattgc gatataggca actcgattga ctgagcaaag
     1861 ttagaaagcg agatcatacc ctcaccagca agcagtgggg cgcagtcact taaagttcga
     1921 taatccaatt gttcatactc ttgtaaggtc tgataccaga ccgccaacag gagcgcggac
     1981 ttctgctttg cttcatgaag atcgtgagtg cctgttgaag tgtgaatctc acactgtcct
     2041 gcgtatcgac gaaaacgttg gggaacgcta atgcggacaa cataaatgcc gctggttcga
     2101 cgatagagtt tatatcgctg agacatcgcc tcgtataccc ttttgtaaga ggggctacgc
     2161 gatttcaagc aagattatga attaaattgt tttaaaacaa tagtttagat gagtcttggt
     2221 gccgaaggcc ggactcgaac cggcacgtat ttctacggtt gattttgaat caactgcgtc
     2281 taccgatttc gccacttcgg cactgaagag gtatgcggaa aacgttgtgg attatacctg
     2341 tcgcacgcca ccatgcaagc gccagcttgc tcgcacctcg ctaagtgctg aaattttcag
     2401 cattgcatac agcgatgtgt aacctttgtc acactccagg caccccgccc tgccatgctc
     2461 tacacttccc aaacaacacc agagaaggac caaaaaatgt cgatgataaa aagctatgcc
     2521 gcaaaagaag cgggcggcga actggaagtt tatgagtacg atcccggtga gctgaggcca
     2581 caagatgttg aagtgcaggt ggattattgc gggatctgcc attccgatct atcgatgatc
     2641 gacaatgaat ggggattttc acaatatccg ctggttgccg ggcatgaggt gattgggcgc
     2701 gtggtggcgc tcgggagcgc cgcgcaggat aaaggtttgc aggtcggcca gcgtgtcggg
     2761 attggctgga cggcgcgtag ctgtggtcac tgcgacgcct gtattagcgg taatcagatc
     2821 aactgcgagc aaggtgctgt gccgacgatt atgaatcgcg gtggctttgc cgagaagttg
     2881 cgtgcggact ggcaatgggt gattccactg ccagaaaata ttgatatcga gtccgccggg
     2941 ccgctgttgt gcggcggtat cacggtcttt aaaccactgt tgatgcacca tatcactgct
     3001 accagccgcg ttggggtaat tggtattggc gggctggggc atatcgctat aaaacttctg
     3061 cacgcaatgg gatgcgaggt gacggccttt agttctaatc cggcgaaaga gcaggaagta
     3121 ctggcgatgg gtgccgataa agtggtgaat agccgcgatc cgcaggcact gaaagccctg
     3181 tcggggcagt ttgatctcat tatcaatact gtgaacgtca gcctcgactg gcagccttat
     3241 tttgaggcgc tgacctatgg cggtaatttc catacggtcg gtgcggttct cacgccgttg
     3301 ccggttccgg cctttacgct gattgcgggc gatcgcagtg tctctggctc tgctaccggt
     3361 acaccttatg agttgcgtaa gctgatgcgc tttgccgccc gcagcaaggt tgcgccgact
     3421 accgaattgt tcccgatgtc gaaaattaac gatgccatca agcatgtgcg cgacggtaag
     3481 gcgcgttacc gcgtggttct gaaagctgac ttctgatctc tttgccgggt gcctgtcgcc
     3541 cggcacttaa atctctttgg aaggcgtctc gcaatctgct gaatttagcg gtgaagagtg
     3601 cgcagccgtt gcctatactc caggcaggaa actggaggat atctcatgag cgaacccctg
     3661 ttaattgccc gcacgccgga cacagaacta tttttactgc cgggaatggc taaccgtcac
     3721 gggctgatta ctggcgcaac ggggacgggt aaaactgtca cgctgcaaaa actggcggag
     3781 tcattgtcgg aaatcggcgt gccggtgttt atggctgatg tgaaaggcga tctgaccggt
     3841 atcgcgcagg caggaacggc gtcggaaaaa ctgctcgcaa ggcttaaaaa tatcgacgtc
     3901 aatgactggc aaccgcatgc taatccggtg gtggtgtggg atatctttgg cgagaaaggc
     3961 cattcggtac gggcgacggt ttcagacctg gggccactgt tgctggtgcg gctgttgaat
     4021 ctcaacgatg tgcaatctgg cgtgctgaat atcatcttcc gcattgctga cgatcagggg
     4081 ctgttactgc tcgactttaa agatctgcgg gcgattaccc agtacatcgg cgataacgcc
     4141 aaatctttcc agaatcagta cggaaatatc agtagcgcat cggttggtgc catccagcgc
     4201 gggctgttgt cgctggaaca gcaaggcgca gcacacttct ttggtgagcc gatgctggat
     4261 atcaaagact ggatgcgcac cgatgccaac ggtaaaggcg ttatcaatat cctcagcgcc
     4321 gagaaacttt atcagatgcc gaaactgtac gccgccagcc tgctgtggat gctctcagag
     4381 ttgtatgaac aattgccgga agcgggcgat ctggagaaac caaaactggt gtttttcttc
     4441 gacgaagcac atctgctgtt taacgatgca ccgcaggtac tgctggataa gattgagcag
     4501 gtgataaggc ttattcgctc aaaaggcgtg ggcgtctggt tcgtttcgca aaacccgtct
     4561 gatattccgg ataatgtgct cggccagctc ggtaatcgcg ttcagcacgc tttgcgggct
     4621 tttacgccca aagatcagaa agcagtaaaa gctgcggcgc aaaccatgcg ggccaatccg
     4681 gcgtttgata ccgaaaaggc gattcaggaa ctgggcaccg gcgaggcgtt gatctctttt
     4741 ctggatgcga aaggaagccc ttctgtggtg gagcgtgcga tggtgatcgc gccttgttcg
     4801 cggatggggc cggtgacgga agatgagcgt aatggcttga ttaatcactc tccggtgtat
     4861 ggcaaatatg aggatgaggt ggaccgggaa tccgcctatg agatgttgca aaaaggcttt
     4921 caggccagta ccgagcagca aaataatccc cccgcgaaag ggaaagaggt agcggtggat
     4981 gacggcattc ttggtggatt gaaggatatt ttgttcggca ctaccggacc acgcggcggg
     5041 aagaaagatg gtgtggtgca aacaatggcc aaaagcgccg ctcgccaagt gacgaatcag
     5101 attgtacgcg ggatgttggg gagtttgctg ggggggagaa gaaggtaagt caggcgagat
     5161 ttgtctttgc ctgatgcgac gctggcgcgt cttatcatgc ccaccccact gcaatatatt
     5221 gaattttaat tatttttcag gaatgaacga agcacacttt gctaacaata ataaagggag
     5281 ctttcgctcc ctttattcgt tcattcggtt acgattttct cattaacagc cacaggctga
     5341 ttaagaagaa gctggcgctt ggcaacagtg cgccgatgat cggcgggatg ccataaacca
     5401 acgtcagcgg accgaagatc tggtccagta cgtagaagac aaaaccgaaa ctgataccgg
     5461 tgaccacacg cacgcccatc ggtacgctac gcagtgggcc aaagatgaac gacagcgcca
     5521 tcagcatcat caccgccacg gatagcggct ggaagatttt gctccacatg ttgagctgat
     5581 aacgtccggc atcctgaccg ctcgacttca gatacttcac atagttgtgc aaaccgctga
     5641 tagagagtgc atccgggtcc agcgccacca cgcccagttt gtccggcgtg aggttggttt
     5701 tccaggtgcc gctcaccgtc tgcgaaccgg taatctgttt cggattggtc agatcagatt
     5761 catcaacctg cgacaaacgc cagactttat gttccgggtc aaacttcgca gtagcggcat
     5821 agcgtacgga ttgcagacga cgattctcgt taaaggcata aatgctgatg ccacctaact
     5881 cttcgtcacc tttaacccgc tcaatgtaga cgaagttgtt gccatctttc gcccataagc
     5941 cttgctgggt agagagcaac gagccgccgt acatcgcctg cgcacggtag ttacgcgcca
     6001 tctgctcgcc ctgcggcgcg acccattcgc caatcgccat cgtcagcaag accagcggaa
     6061 tggcggtttt catcaccgac agcgccacct gcatacgggt aaaaccagaa gcctgcatca
     6121 ccaccagttc gctgcgctgc gccagcatcc caagaccaag caacgcccca agcagagccg
     6181 ccatcgggaa gaatatttgc acatctttcg gcacgctcag caaggtatac attcctgcgc
     6241 ctaacgcgtc gtaactcccc tgcccggctt ttttcaactg atcgacaaac ttgataatgc
     6301 ccgacagcga caccagcatg aacagcgtca tcatgatggt ggtgaaaata gttttaccga
     6361 tatagcggtc aagtacgcca aaaggttgca tcacaccgct cctttacgcg aaaaactggc
     6421 gcgcaggcgg cggaccggca cggtgtccca aaggttgaga acaatcgcca aagccagata
     6481 aatcaggtta acggtccaca tccacagcgt cgggtccagc ttacctttac cgccgttcga
     6541 tttcagggag gtctggatca ggaagaaaag tagatacagc agcatggctg gcagcatcga
     6601 cagtacgcgt ccctgacgtg ggttaaccac gctcagcggt acgaccataa gtgccatcat
     6661 aaacacggtg aataccaacg tgatacgcca gttcagttct gcgcgagcac gatcggtgtc
     6721 agtgttccac aatgtgcgca tgtccatctg gtcggtatcg ttcgggtcga gcgccaccgc
     6781 ctggtgacca atgatcgcct gataatcctg gaaatccgta atgcggaaat cacgtaacag
     6841 tgcagtgcct tcgaagcgcg ttccctggtt gagagtgacg acctgggagc cgtcgcgcag
     6901 ctgggttaaa tgtccggaat cggccaccac cacagaagga cgtgcattac cttttggtcg
     6961 aatttgcgcg aggaacacat ctttgaaatc gctgccgtca acgctttcga tgaacagcac
     7021 cgaactgcca ttagtcgctt gctggaattg cccttgcgcc agcgccgcca tgccagggtt
     7081 cgctttcgct tctgctaaca cttcatcctg atgacgcgat gaccacggtc ccgcccacat
     7141 cacgttaacc gccgcgacga ttgccgtgaa taccgcaagg atcattgccg ctttcaccag
     7201 aaccgctttg ctcaggccgc aggcatgcat taccgtaatt tcactttcgg tatacagttt
     7261 gcccagcgtc atcagcagcc cgaggaacag gcttaatggc aggataagct gcgccatttc
     7321 cggcacgccc aacccgagaa gggagagcac cagattcgcc ggaatatcgc cgtcaaccgc
     7381 tgcgccgagg atcctcacta acttttgaca gaagaagatc aaaagcaaga tgaagagtat
     7441 cgccagctgg cttttgagcg tctcccgcac cagatatctt atgattatca ctttaaatac
     7501 gcccgtaaaa actcgtcttt tgcaggattt tagcttgttt catggcttaa acgtcattta
     7561 ttctcttgag tcgtcgaaat cgtcgctaag ataattatac tcaacggatt cacctctcag
     7621 attttgttct gacgtgccaa tgccgtaata acgttaagat taacacgaag tcatcgcaac
     7681 agcggacatg agttacgaaa gcttgcaatt ctatctgtag ccaccgccgt tgtctttaag
     7741 attcaggagc gtagtgcatg gagtttagtg taaaaagcgg tagcccggag aaacagcgga
     7801 gtgcctgcat cgtcgtgggc gtcttcgaac cacgtcgcct ttctccgatt gcagaacagc
     7861 tcgataaaat cagcgatggg tacatcagcg ccctgctacg tcggggcgaa ctggaaggaa
     7921 aaccggggca gacattgttg ctgcaccatg ttccgaatgt actttccgag cgaattctcc
     7981 ttattggttg cggcaaagaa cgtgagctgg atgagcgtca gtacaagcag gttattcaga
     8041 aaaccattaa tacgctgaat gatactggct caatggaagc ggtctgcttt ctgactgaac
     8101 tgcacgttaa aggccgtaac aactactgga aagtgcgtca ggctgtcgag acggcaaaag
     8161 agacgctcta cagtttcgat cagctgaaaa cgaacaagag cgaaccgcgt cgtccgctgc
     8221 gtaagatggt gttcaacgtg ccgacccgcc gtgaactgac cagcggtgag cgcgcgatcc
     8281 agcacggtct ggcgattgcc gccgggatta aagccgcgaa agatctcggt aatatgccgc
     8341 cgaatatctg taacgccgct tacctcgctt cacaagcgcg ccagctggct gacagctaca
     8401 gcaagaatgt tatcacccgc gttatcggcg aacagcagat gaaagagctg gggatgcatt
     8461 cctatctggc ggtcggtcag ggttcgcaaa acgaatcgct gatgtcggtg attgagtaca
     8521 aaggcaacgc gtcggaagat gcacgcccaa tcgtgctggt gggtaaaggt ttaaccttcg
     8581 actccggcgg tatctcgatc aagccttcag aaggcatgga tgagatgaag tacgatatgt
     8641 gcggtgcggc agcggtttac ggcgtgatgc ggatggtcgc ggagctacaa ctgccgatta
     8701 acgttatcgg cgtgttggca ggctgcgaaa acatgcctgg cggacgagcc tatcgtccgg
     8761 gcgatgtgtt aaccaccatg tccggtcaaa ccgttgaagt gctgaatacc gacgctgaag
     8821 gccgcctggt actgtgcgac gtgttaactt acgttgagcg ttttgagccg gaagcggtga
     8881 ttgacgtggc gacgctgacc ggtgcctgcg tgatcgcgct gggtcatcac attaccggtc
     8941 tgatggcgaa ccataatccg ctggcccatg aactgattgc cgcgtctgaa caatccggtg
     9001 accgcgcatg gcgcttaccg ctgggtgacg agtatcagga acaactggag tccaattttg
     9061 ccgatatggc gaacattggc ggtcgtcctg gtggggcgat taccgcaggt tgcttcctgt
     9121 cacgctttac ccgtaagtac aactgggcgc acctggatat tgcaggaacc gcctggcgtt
     9181 ctggtaaagc aaaaggcgca accggtcgtc cggtagcgtt gctggcacag ttcctgttga
     9241 accgcgctgg gtttaacggc gaagagtaat tgcgtcaggc aaggctgtta ttgccggatg
     9301 cggcgtgaac gccttatccg acctacacag cactgaactc gtaggcctga taagacgcgc
     9361 aagcgtcgca tcaggcaatg ctgttattgt cggatgcggc atgaacgcct tatccgacct
     9421 acacagcacc gaactcgtag gcctgataag acgcggcagc gtcgcatcag gcaatgctgt
     9481 tattgtcgga tgcggcgtga gcgccttatc cgacctacac acagcactga actcgtaggc
     9541 ctgataagac acaacagcgt cgcatcaggc gctgcggtgt atacctgatg cgtatttaaa
     9601 tccaccacaa gaagccccat atatgaaaaa cgcgacgttc taccttctgg acaatgacac
     9661 caccgtcgat ggcttaagcg ccgttgagca actggtgtgt gaaattgccg cagaacgttg
     9721 gcgcagcggt aagcgcgtgc tcatcgcctg tgaagatgaa aagcaggctt accggctgga
     9781 tgaagccctg tgggcgcgtc cggcagaaag ctttgttccg cataatttag cgggagaagg
     9841 accgcgcggc ggtgcgccgg tggagatcgc ctggccgcaa aaacgcagca gcagcccgcg
     9901 cgatatattg attagtctgc gaacaagctt tgcagatttt gccaccgctt tcacagaagt
     9961 ggtagacttc gttccttatg aagattctct gaaacaactg gcgcgcgaac gctataaagc
    10021 ctaccgcgtg gctggtttca acctgaatac ggcaacctgg aaataatgga aaagacatat
    10081 aacccacaag atatcgaaca gccgctttac gagcactggg aaaagcaggg ctactttaag
    10141 cctaatggcg atgaaagcca ggaaagtttc tgcatcatga tcccgccgcc gaacgtcacc
    10201 ggcagtttgc atatgggtca cgccttccag caaaccatca tggataccat gatccgctat
    10261 cagcgcatgc agggcaaaaa caccctgtgg caggtcggta ctgaccacgc cgggatcgct
    10321 acccagatgg tcgttgagcg caagattgcc gcagaagaag gtaaaacccg tcacgactac
    10381 ggccgcgaag ctttcatcga caaaatctgg gaatggaaag cggaatccgg cggcacgatt
    10441 acccgtcaga tgcgccgtct cggcaactcc gtcgactggg aacgtgaacg cttcacgatg
    10501 gacgaaggcc tgtccaatgc ggtgaaagaa gttttcgttc gtctatataa agaagacctg
    10561 atttaccgtg gcaaacgcct ggtaaactgg gacccgaaac tgcgcaccgc tatctctgac
    10621 ctggaagtgg aaaaccgcga atcgaaaggt tcgatgtggc acatccgcta tccgctggct
    10681 gacggtgcga aaaccgcaga cggtaaagat tatctggtgg tcgcgactac ccgtccggaa
    10741 accctgctgg gcgatactgg cgtagccgtt aacccggaag atccgcgtta caaagatctg
    10801 attggcaaat atgtcattct gccgctggtt aaccgtcgta ttccgatcgt tggcgacgaa
    10861 cacgccgaca tggaaaaagg caccggctgc gtgaaaatca ctccggcgca cgactttaac
    10921 gactatgaag tgggtaaacg tcacgcgctg ccgatgatca acatcctgac ctttgacggc
    10981 gatatccgtg aaagcgccca ggtgttcgat accaaaggta acgaatctga cgtttattcc
    11041 agcgaaatcc cggcagagtt ccagaaactc gagcgttttg ctgcacgtaa agcggtcgtt
    11101 gctgcggttg acgcactcgg cctgctggaa gaaattaaac cgcacgacct gacagttcct
    11161 tacggcgacc gtggcggcgt agttatcgaa ccaatgctga ccgaccagtg gtacgtacgt
    11221 gccgatgtac tggcgaagcc ggcagttgaa gcggttgaga acggcgacat tcagttcgta
    11281 ccgaagcagt acgaaaacat gtacttctcc tggatgcgcg atattcagga ctggtgtatc
    11341 tctcgtcagc tgtggtgggg tcaccgtatc ccggcatggt acgacgaagc gggtaacgtt
    11401 tatgttggcc gcaacgaaga agaagtgcgc aaggaaaata acctcggtgc cgacgttgcc
    11461 ctgcgtcagg acgaagacgt tctcgacacc tggttctctt ctgcgctgtg gaccttctct
    11521 accctaggct ggccggaaaa caccgacgca ctgcgccagt tccacccaac cagcgtgatg
    11581 gtatctggtt tcgacatcat cttcttctgg attgcccgca tgatcatgat gaccatgcac
    11641 ttcatcaaag atgaaaatgg caaaccgcag gtgccgttcc acaccgttta catgaccggt
    11701 ctgattcgtg acgacgaagg ccagaagatg tccaaatcca agggtaacgt tatcgacccg
    11761 ctggatatgg ttgacggtat ttcgctgcca gaactgctgg aaaaacgtac cggcaatatg
    11821 atgcagccgc agctggcgga caaaatccgt aagcgcaccg agaagcagtt cccgaacggt
    11881 attgagccgc acggcaccga tgccctgcgc ttcaccctgg cggcactggc gtctaccggt
    11941 cgtgacatca actgggatat gaagcgtctg gaaggctacc gtaacttctg taacaagctg
    12001 tggaatgcca gccgctttgt gctgatgaac acggaaggtc aggattgcgg cttcaacggc
    12061 ggcgaaatga cgctgtcgct ggcggaccgc tggattctgg cggagttcaa ccagaccatc
    12121 aaagcgtacc gcgaagcgct ggacagcttc cgcttcgata tcgccgcagg cattctgtat
    12181 gagtttacct ggaaccagtt ctgtgactgg tatctcgagc tgaccaagcc ggtaatgaac
    12241 ggtggcaccg aagcagaact gcgcggtact cgccatacgc tggtgactgt actggaaggt
    12301 ctgctgcgcc tcgcgcatcc gatcattccg ttcatcaccg aaaccatctg gcagcgtgtg
    12361 aaagtacttt gcggtatcac tgccgacacc atcatgctgc agccgttccc gcagtacgat
    12421 gcatctcagg ttgatgaagc cgcactggcc gacaccgaat ggctgaaaca ggcgatcgtt
    12481 gcggtacgta acatccgtgc agaaatgaac atcgcgccgg gcaaaccgct ggagctgctg
    12541 ctgcgtggtt gcagcgcgga tgcagaacgt cgcgtaaatg aaaaccgtgg cttcctgcaa
    12601 accctggcgc gtctggaaag catcaccgtg ctgcctgccg atgacaaagg tccggtttcc
    12661 gttacgaaga tcatcgacgg cgcagagctg ctgatcccga tggctggcct catcaacaaa
    12721 gaagatgagc tggcgcgtct ggcgaaagaa gtggcgaaga tcgaaggcga aatcagccgt
    12781 atcgagaaca agctggcgaa tgaaggcttt gtcgcccgcg caccggaagc ggtcatcgcg
    12841 aaagagcgtg agaagctgga aggctatgcg gaagcgaaag cgaagctgat tgaacagcag
    12901 gctgttatcg ccgcgctgta atcaaaacac agtgatgaaa acgaaggccg gagcatgctc
    12961 cggccttttt atctcttaca gaaatggtaa agaaggcata attccccgtg aaatactggc
    13021 taagaagcct ttatctggtt gttcttcttt attgattaaa ggaaggtcat ccaaatcgcc
    13081 cagcaaatac gtattttttg cctgccagtc aatcatccat attttcagca gtggataagc
    13141 cagaccgccc gtaatcccgg atatcaccac caacgcacac atgcgataaa gcataccgtg
    13201 gtatgttaaa gttgagcgaa aacgaatacg cccatcattc agtgacaggt tgctcataaa
    13261 atgatttcgc aacgacaccg ttaaataact ggtgctaaca gcaatcccaa aataatagat
    13321 taactgcgcg
//
LOCUS       DBFIFI010000115        13310 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.115, whole genome shotgun sequence.
ACCESSION   DBFIFI010000115 DBFIFI010000000
VERSION     DBFIFI010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13310
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.115"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            42..1100
                     /locus_tag="N7B39_003134"
     CDS             42..1100
                     /locus_tag="N7B39_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HGU2423072.1"
                     /translation="MNKKIWFMEGLSSQRDIIQGVKSFAQKNNFAITVFASHRNERNE
                     ILSVADYSLTEPEDPQKRLQFIQETIQTYGIHHIHTGRNSQWFEEHRSAIEPTGATLT
                     TGATGVDWLTLADEKVTFAQFMEQKGLPVVPSWRVNTLAELKTHLAAPPFTDSPVCVK
                     PVTGIYGMGFWRFDDSASPMAVFNHPEHRLVSPQQYIAAASAAESFKPLVLMPYLPGP
                     EFSVDILADKGEILAAVGRRKEGAIQYLVNEGSAWELACDCARVMKADGLVNVQTRND
                     VNGNPVLLETNMRPSGGVGYTLHSGVNLPGLFAAFKLGLMSEDMVRQSAKNTFSPVAV
                     RSITDVIAYPESLSNLLN"
     gene            1110..2252
                     /locus_tag="N7B39_003135"
     CDS             1110..2252
                     /locus_tag="N7B39_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase domain-containing
                     protein"
                     /protein_id="HGU2423073.1"
                     /translation="MSDILEDMIYRRTLSCGTIQVTRDQGEVSLDDLFDIAERRNPKR
                     AFLFVSKVLGRHIPVPPSVMRQAYRQLASQFPSTLTGPVLFIGMAETAVGLGAGVFDE
                     VRHQHRESVYLTSTRHPVDGTLLCEFKEEHSHATDHLIYLPDDEEKRRRVTNARTLVL
                     IDDEATTGNTFINLLSALRNTGKLQHIEQVIAVTLTDWSGNALSERTPLPVTSVSLVS
                     GQWGWTPLPDAPVPDMPKVNVTSRGEWDIQGKQSWGRLGMLAPAADLGHEVSVHKGER
                     ILVLGTGEFVWEPFLLAERLEAAGAQAFYGSTTRSPVAVGYAIESAISFTDNYGLGIP
                     NFVYNVAHQQFDRILVCTETPAESIDTQLLKALAEVAPVVEIVTYE"
     gene            2245..3018
                     /locus_tag="N7B39_003136"
     CDS             2245..3018
                     /locus_tag="N7B39_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2423074.1"
                     /translation="MNKPVVLSDLDDTLFQTRRKMVDELALEPFRTGAVDRTLNPRSF
                     MTEEQSMLVDWLLEQAELIPVTARGTEEISRVRIPFHSWAITTHGAVILTPEGKPDEE
                     WKAHMLGQLAPYQEKLTSMQRLITEMMDAKGINAWARLNFEYGETAVYMVMKHRDSTR
                     LDELNAIADEIETVFPTEGFYIHRNSNNVAWLPTPVEKGLAVSWLLEKLRAERGVFPV
                     IGLGDSLSDHRFMKLCSWFGIPRQSQFADAISQRIFGEN"
     gene            3020..4099
                     /locus_tag="N7B39_003137"
     CDS             3020..4099
                     /locus_tag="N7B39_003137"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HGU2423075.1"
                     /translation="MQPHETFTGSYQPGDVEFLLKPVVIEMTPVEKKEELIQSGKKHY
                     SDMLSQEPAPTQWHLDLFHRALDRGAERLAKEVTQLAIALAERFGDEPIVLASLVRAG
                     VPLGVMLHQALRDMGKTSWHYGISIIRDRGIDGAALDVIEERHGTSGIVFVDGWTGKG
                     AITGELVRALKDRPGYPEQPRLVVLADPCGCSWLAASDDDWLIPFGIMGAPVSGLISR
                     SVWSSEGLHGCMVCEHLSEFECSRMLADTVAHFRKKLTPSSLAPLSWNTESARVLWQT
                     SRDVIAFLADEFKVDSVNRIKPGIAEATRAVLRRVPDHVFVRSIDDPDVALLVGLARE
                     KGIVVTEMGGTLGQYRAVTIIKKVL"
     gene            4099..5055
                     /locus_tag="N7B39_003138"
     CDS             4099..5055
                     /locus_tag="N7B39_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HGU2423076.1"
                     /translation="MKNRLSPWNLGATLYMPATREDIADAVLHGKIPGLRSLVICLED
                     AVSEADIPVALKNLEHLLHELSNSMSSLGKNDWPLVFIRPRHAEMGRWLKAHYDLSAV
                     DGFVLPKFTLSSLAEWWDIMVGTHLCMMPTLETEDVFDVVQMRELATRLEEHPCHDRI
                     IALRIGGNDLMNVVSLRRPRDLTLYDSPMGYVIKMLVSVFGPRDFALTAPVCEHIDDH
                     AVMARELALDMAHGLVGKTAIHPGQIEVIQNALMVTQGEHSDALRILNSTQAVFKSQG
                     AMCEPATHRRWAAGILDRARFYGLQNEQSADGIRLLTVTQHH"
     gene            5066..6274
                     /locus_tag="N7B39_003139"
     CDS             5066..6274
                     /locus_tag="N7B39_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HGU2423077.1"
                     /translation="MGFRFRKSINIIPGVRLNLSNGAPSLSVGPRGASVSFGSRGTYA
                     NLGLPGTGLSYRTRLDRAARSRGENRTATDPGLRQALEQKAAELMSAVTAIRNIHELT
                     PDPKTGISWAELEAVYLHNRTSPFQVPAPVRPEKPDYLVLPEKPAESEGISFLGKWFE
                     SESAKAERHAENLRRWQQELIDVERENTLRQHRYQQQRTAWAEQYANWKFEAEEHEKR
                     LATAQADARQQFRTDAAFFESYLAGVLAETEWPRETLVAFEVKPELSAVLLDVDLAEI
                     EDFPDKIYGVNARGTELTEKAMTQKAVRENYAHHVHGCLFRLVGIVLHTLPFDNVIVS
                     GFTQRVSKRTGYLEDEYILSCKCTRSQMSSVNFAGIKHIDPVEALGDQPVIRKMSSTF
                     IFQPIEPLTL"
     gene            6292..6759
                     /gene="terW"
                     /locus_tag="N7B39_003140"
     CDS             6292..6759
                     /gene="terW"
                     /locus_tag="N7B39_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance protein TerW"
                     /protein_id="HGU2423078.1"
                     /translation="MQLNTRQARIFKLANLLGTGKPVSAADIITSLECSEPTLTRALK
                     ELRESYSAEIKYSKAGHSYHLVNPGQLDKKTLRRMNEALAQNAELKTGESTGKVVLDK
                     DKKTAVSLSLRMRILRKIDRLAALSDSTRSEAVEKLALHSVDELIKEYSAKKS"
     gene            7020..7349
                     /locus_tag="N7B39_003141"
     CDS             7020..7349
                     /locus_tag="N7B39_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGU2423079.1"
                     /translation="MSKPRWTLDQKKHHVAAWRASGLTREQYCELYDIPFKSLRQWPQ
                     DVAKAEKRARAPEIIPVSVSGSSGMTDGRPLSDEPVTLFLPGGIRMCCQPSQLTDVFR
                     ALRHADA"
     gene            7336..7677
                     /gene="tnpB"
                     /locus_tag="N7B39_003142"
     CDS             7336..7677
                     /gene="tnpB"
                     /locus_tag="N7B39_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309368.2"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGU2423080.1"
                     /translation="MLTPDNVFLVVKPVDMRRGIDTLTQYVQNELNAAWHDGAAFVFT
                     NKVRSRIKVLRWDKHGVWLCTRRLHRGSFRWPRKGDATWHLTQDEFHWLVFGVDWQQV
                     KGHDLAKWVYQ"
     gene            7759..8589
                     /locus_tag="N7B39_003143"
                     /pseudo
     CDS             7759..8589
                     /locus_tag="N7B39_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(8620..10233)
                     /locus_tag="N7B39_003144"
     CDS             complement(8620..10233)
                     /locus_tag="N7B39_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element IS682 family transposase"
                     /protein_id="HGU2423081.1"
                     /translation="MSRKYLIRITELERLLSEQAEALRQKDQQLSLVEETEAFLRSAL
                     ARAEEKIEEEEREIEHLRAQIEKLRRMLFGTRSEKLQREVEQAEAQLKQREQESDRYS
                     GREDDPQVPRQLRQSRHRRPLPAHLPREIHRLEPEESCCPECGSELDYLGEVSAEQLE
                     LVSSALKVIRTVRVKKACTKCDCIVEAPAPSRPIERGIAGSGLLARVLTGKYCEHLPL
                     YRQSEIFARQGIELSRALLSNWVDACCQLMTLLNDTLYRYVMNTRKVHTDDTPVKVLA
                     PGRKKAKTGRIWTYVRDDRNAGSSEPPAVWFAYSPDRQGKHPVQHLRPFRGILQADAF
                     SGYDRLFSAEREGGALTEVACWAHARRKIHDVYISSKSATAEEALKRISELYAIEDEI
                     RGLPESERLAVRQQRSKVLLTSLHEWMVEKNGTLSKKSRLGEACSYVLNQWDALCYYS
                     DDGLAEADNNAAERALRAVCLGKKNFMFFGSDHGGERGALLYGLIGTCRLNGIDPEAY
                     LRHILSVLPEWPSNRVDELLPWNVVLTNK"
     gene            complement(10264..10614)
                     /gene="tnpB"
                     /locus_tag="N7B39_003145"
     CDS             complement(10264..10614)
                     /gene="tnpB"
                     /locus_tag="N7B39_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGU2423082.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLDENPFSGHLF
                     IFRGRRGDTIKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            complement(10611..11036)
                     /locus_tag="N7B39_003146"
     CDS             complement(10611..11036)
                     /locus_tag="N7B39_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU2423083.1"
                     /translation="MEQKILSSEPRRSFSNEFKLQMVKLASQPGASVARIAREHDIND
                     NLLFKWLRLWQNERRISRRLPVTTSSGAGVELLPVEITPDEQKEPMAALTPLLSTPSQ
                     STVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            11090..11841
                     /locus_tag="N7B39_003147"
                     /pseudo
     CDS             11090..11841
                     /locus_tag="N7B39_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            12181..12354
                     /locus_tag="N7B39_003148"
     CDS             12181..12354
                     /locus_tag="N7B39_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2423084.1"
                     /translation="MSSPEQYLSSSEPASDTSLAVAPAQQNKSTIVRLRSFVCIADDA
                     PEKRVNMSSPEYY"
BASE COUNT         3191 a         3509 c         3486 g         3124 t
ORIGIN      
        1 tcaatcataa atttgcatgg cgttccggat aattaaaaaa aatgaacaag aaaatttggt
       61 ttatggaagg tttatcctcc cagcgagata ttattcaggg ggtaaaatca tttgcacaaa
      121 aaaataattt tgccattacc gtttttgcct cccaccgtaa cgaaagaaat gaaatccttt
      181 ccgttgccga ttattctttg actgaacctg aagatcctca aaaacgtctt cagtttatcc
      241 aggaaaccat tcagacctac ggcatccacc atattcatac tggccgtaac agccagtggt
      301 ttgaagaaca ccgttcagcc attgaaccga ccggtgccac ccttactacc ggtgcaacgg
      361 gcgtcgactg gttaactctg gctgacgaaa aagttacttt tgctcagttt atggagcaaa
      421 agggtctccc ggtcgtacca tcctggcggg tgaatacgct ggcagaatta aagacacacc
      481 tcgcggcccc gccgttcact gacagcccgg tatgcgtgaa gccggtgacg ggtatctatg
      541 gcatgggatt ctggcgcttt gatgacagtg cttcgcctat ggccgtcttt aatcatcccg
      601 aacatcgtct ggtcagtccg caacagtata ttgcagcagc atcagctgct gagtcgttta
      661 aaccccttgt tttgatgccg tacctgccag gcccggaatt ttccgtcgat atcctcgcgg
      721 ataagggcga aatactcgca gccgtgggac gccgtaagga aggggctatc cagtatctgg
      781 taaacgaagg aagcgcctgg gaactggcgt gtgactgcgc ccgtgttatg aaggccgacg
      841 ggctggtgaa tgttcagacg cgaaacgatg tgaatggcaa cccggtgctg cttgaaacca
      901 acatgcgtcc gtcagggggg gtgggttata cccttcacag cggtgtgaac cttcctgggt
      961 tatttgctgc ctttaagctc ggtctgatgt ctgaagatat ggtacgccag agcgctaaaa
     1021 acaccttttc tccggttgcg gtgagatcca ttacggatgt aattgcatac ccggaatcac
     1081 tctctaacct tctgaattaa ggcgtattta tgtctgatat cctcgaagat atgatttatc
     1141 gccgtaccct ctcatgcggt acgattcagg taacccgtga ccagggtgaa gtttcgctcg
     1201 atgacctgtt tgacatcgct gaaagacgta acccgaaacg cgcctttctg tttgtcagca
     1261 aagtactcgg caggcacatt cccgtgccac cctcagtcat gcggcaggcc tacagacagc
     1321 ttgccagcca gttcccctcg acactgacag gacccgtact gtttatcggt atggctgaaa
     1381 ccgccgttgg gcttggggcc ggtgtatttg atgaagtgcg ccaccagcat cgtgaatctg
     1441 tctatctgac ctctacccgt cacccggttg atggcacgtt gctttgcgag ttcaaggaag
     1501 aacacagcca cgccaccgat catctgatct atctgccaga tgatgaagag aaaagacgtc
     1561 gtgttaccaa cgcacgaacg ctggttttga ttgatgacga ggcaaccacc ggtaatacct
     1621 ttattaacct gctttctgcc ctgcgtaata ccggcaagct tcaacatatt gaacaggtta
     1681 tcgccgtaac gctgaccgac tggagtggca atgccctgtc ggagcgcacc cccttgcccg
     1741 tcacttcggt ttcgcttgta agcggtcagt ggggatggac cccattacct gatgcacctg
     1801 tcccggacat gccgaaagtc aacgtaactt cacgaggaga atgggacatc caggggaaac
     1861 agtcatgggg ccgactgggg atgctcgcac ctgcggccga tctcggccat gaggtctccg
     1921 tccacaaggg ggaacgtatt ctggttctcg ggaccgggga attcgtctgg gagccgttcc
     1981 tgcttgctga acggctcgaa gctgccggag cacaggcatt ttatggatcg accacccgct
     2041 cccctgtcgc cgttggttat gccattgagt ccgccatttc ctttacggat aactacgggc
     2101 tgggcatccc caattttgtc tataacgtcg cccaccagca gtttgaccgc attcttgtgt
     2161 gtactgagac acccgcagaa agtattgaca cgcagcttct taaagcgctg gctgaggttg
     2221 cgcccgtcgt ggagattgtt acctatgaat aaacccgttg ttctgagcga ccttgacgac
     2281 acgctctttc agacccgtcg taaaatggtg gacgagctgg ccctggagcc attccgtacc
     2341 ggtgccgttg atcgcaccct gaatccacga agctttatga cggaagaaca gtccatgttg
     2401 gtggactggc tcctggagca ggctgaactc atacccgtta ccgcacgggg aactgaagaa
     2461 atcagccgcg tacggatccc tttccactcc tgggcaatca caacgcacgg ggccgtcatt
     2521 ctcacgcctg aaggcaaacc cgacgaggag tggaaggccc atatgctcgg ccagctggct
     2581 ccctaccagg aaaagctgac atcgatgcag cgtctgatca ctgaaatgat ggacgcaaaa
     2641 ggaatcaatg cctgggcaag gctgaacttc gaatacggtg aaacggcggt ttatatggtg
     2701 atgaaacacc gcgacagcac ccgccttgac gagctcaatg ccattgcaga tgagatagaa
     2761 acggtgtttc cgaccgaggg cttctacatc caccgcaaca gtaataacgt ggcctggctt
     2821 cccaccccgg ttgagaaagg gctggcagtc agctggctgc ttgaaaaact tcgggctgaa
     2881 cgaggagttt tccccgtaat tggtttgggc gacagcctga gcgatcatcg ttttatgaaa
     2941 ctgtgcagct ggtttggcat cccgcgtcag agtcagtttg cagatgccat ttcacagcga
     3001 atttttggag aaaattaaaa tgcagccaca tgaaacattt accggctcat accagcccgg
     3061 tgacgtggaa tttctgctaa agccggttgt cattgagatg acgccggttg agaaaaaaga
     3121 agaactgatt cagtccggga agaaacatta ttcggacatg ctcagccagg agccagcgcc
     3181 tacacaatgg catcttgatt tgtttcaccg ggcgctggat cggggcgcag agaggcttgc
     3241 aaaagaagtc acacagctcg ctattgctct ggccgaacgc ttcggtgatg agcccattgt
     3301 actggccagt cttgtcaggg ctggcgttcc gctcggcgtt atgctgcacc aggccctgcg
     3361 tgacatgggg aaaacctcat ggcattacgg tatcagcatt atccgggatc gtggaattga
     3421 cggtgcagcc ctcgacgtca ttgaagagcg gcatggtacc agcggtattg tctttgttga
     3481 cggatggaca ggtaaaggcg caattaccgg agagcttgta cgcgcactga aagatcgccc
     3541 gggctatcct gagcagccgc gactggttgt cctggccgat ccctgtggct gctcctggct
     3601 tgcagccagt gatgatgact ggctcattcc ttttggcatc atgggggcgc cggtatcagg
     3661 cctgatctcc cggtcagtat ggtcctctga aggattacac gggtgcatgg tttgcgagca
     3721 tctcagtgaa ttcgaatgca gccggatgct tgccgatacc gtcgctcatt tccgtaagaa
     3781 gttaacaccg tcatccctcg ctcccctgag ctggaatacg gaatcagccc gggtcttatg
     3841 gcagacaagt cgcgacgtta tcgcgttcct ggccgatgaa tttaaagtgg acagcgtcaa
     3901 tcgtattaaa cccggtattg ctgaagcaac ccgggctgta ttacgtcggg taccggacca
     3961 tgtatttgtg cgttctattg acgacccgga cgttgccttg cttgtagggc ttgctcgtga
     4021 aaagggaata gttgttacag aaatgggggg aaccctcggc cagtatcggg ctgtaaccat
     4081 tatcaagaag gtactctgat gaaaaatcgt ctttctccct ggaatctggg agccacgcta
     4141 tacatgcctg caacacggga agatattgct gatgccgtcc tgcacgggaa aatcccgggc
     4201 ttgcgctctc tggtgatttg cctggaggat gctgtcagtg aagcagacat ccccgtcgcc
     4261 ctcaaaaatc tggagcacct gctgcacgag ctcagtaaca gcatgagtag cctgggtaaa
     4321 aatgactggc ctctggtgtt cattcgcccc cggcatgccg aaatgggcag atggttaaaa
     4381 gcgcattatg atctttccgc tgtcgacggg tttgtgctgc ctaaatttac cctcagttcg
     4441 ttagctgaat ggtgggatat catggtcggg actcacctgt gcatgatgcc aacgctggaa
     4501 acagaagatg tctttgacgt ggttcagatg cgcgagctgg ctacccgtct ggaggaacat
     4561 ccctgccacg accgcattat tgcgcttcga atcggcggca acgatcttat gaacgttgtg
     4621 tcgcttcgcc gtccccggga cctgacgctg tatgacagcc cgatgggcta cgtcatcaaa
     4681 atgctcgttt cggtcttcgg cccgcgtgat tttgccctga ccgcgcctgt atgtgagcat
     4741 attgacgatc atgccgttat ggccagagag ctggctcttg atatggcaca cgggcttgtt
     4801 gggaaaacgg ccattcaccc gggtcagata gaggtcattc aaaacgcgct gatggtcact
     4861 cagggtgagc attctgacgc tctgcggatc ctgaactcta cccaggccgt gtttaagtcg
     4921 cagggagcaa tgtgtgaacc cgccacacat cgccgctggg cggctggcat tctggacagg
     4981 gctcgttttt atgggttaca gaacgagcaa agcgctgatg gaatcagatt acttaccgtg
     5041 acccagcatc attaaggaaa tcataatggg gttcaggttc agaaagtcga tcaacattat
     5101 tcctggcgtt cgcctcaacc tgagtaacgg tgcaccgagc ctgagtgtcg ggccgagagg
     5161 tgcttccgtt tcttttggta gccgggggac ctatgccaat ctgggcttgc ccggtaccgg
     5221 gctgagttac cgtacccggc ttgaccgggc cgcgcgttcc agaggtgaaa accggacggc
     5281 aaccgacccg gggctcagac aggcgcttga gcagaaagcc gctgaactca tgtcagcggt
     5341 aaccgcaatc cgtaatattc acgagctgac gccggatcca aaaacaggca tcagctgggc
     5401 agagctggaa gcagtatacc tgcataacag aacgtcgcct tttcaggttc cggcaccggt
     5461 gcgtccagaa aagccagact accttgtatt gccggaaaag cctgccgaga gcgaaggcat
     5521 tagttttttg ggtaaatggt ttgaatcgga atcagctaaa gctgagcgcc acgccgaaaa
     5581 tcttcgccgg tggcagcagg agctgattga tgtggagcgt gagaataccc ttcgacagca
     5641 ccggtaccag caacaacgga cggcctgggc cgaacagtat gcaaactgga agtttgaagc
     5701 tgaagaacat gaaaaacggc tcgccacggc tcaggcagat gcccggcagc agttccggac
     5761 agacgccgcg tttttcgaat catacctggc gggtgtgctg gcagaaactg aatggccgcg
     5821 tgaaacgctt gttgcatttg aagtaaagcc ggagctatca gcagtcctgc tggacgttga
     5881 tttagctgag attgaagatt tccctgataa gatttacggc gttaatgccc ggggaacgga
     5941 gctgacggaa aaagccatga cgcaaaaagc cgtacgcgaa aactatgccc accacgtcca
     6001 tggctgcttg ttccgcctgg tcggtatcgt tttacatacg ctacctttcg acaacgtgat
     6061 tgtgtcaggc tttacgcaac gggtcagtaa gcggaccggc tatctggagg atgagtatat
     6121 cctgtcctgc aaatgcactc gcagccagat gtcgtcagta aattttgcag gcataaaaca
     6181 cattgatccg gttgaagcgt taggcgatca accggttatt cgaaagatga gcagtacctt
     6241 catttttcag cctattgaac cactaaccct ttaagcaggc taaatcttac gatgcaatta
     6301 aataccagac aggcccggat ttttaagctt gccaacttac tgggcaccgg caagcccgtt
     6361 tccgctgccg acattataac cagtctggaa tgctctgagc cgacattaac ccgggccctc
     6421 aaagagctac gcgagtcata ttctgcggag atcaaataca gcaaggcagg tcattcctat
     6481 catctcgtta atccgggcca gttggataaa aagaccctcc gccggatgaa tgaagcgctc
     6541 gcacagaatg ctgaactgaa aaccggtgag tccacaggga aggtcgtact cgataaggat
     6601 aaaaaaacag ccgtgtcatt atcgctacgc atgcggattc taagaaaaat tgaccgtctg
     6661 gctgcgctga gtgactcgac ccgaagtgaa gcggtagaga agctggctct gcactccgtt
     6721 gatgaactga taaaagaata cagcgctaaa aagtcctgac gctaaatctc agaccggggt
     6781 gaaaactccg gttttgtcat atttcacctc tgtattggcg tgatgcaaaa tggcatctga
     6841 atcgatcatg gtttggcatc catttccgat cactgtttgg catcacaatc gatcacgatt
     6901 tggcatgctt ccgctcattg attggcatcc tggtgtcgtt acggggaagt catcttcaca
     6961 gggatcttca gtcagttagc attaccgaca tccattcacc aacttaccgg agataatcaa
     7021 tgtcaaaacc ccgatggacg cttgatcaga aaaaacatca cgttgccgca tggcgcgcca
     7081 gcggccttac ccgtgaacag tactgtgaac tttacgacat tccgtttaaa tccctgcgcc
     7141 agtggccgca ggatgtcgca aaagccgaaa aacgagccag agcaccagaa attattcctg
     7201 tttcagtatc tggctcttcc ggaatgactg atggcagacc attgtcggat gagcctgtca
     7261 ctctcttcct gcccggtggt atccggatgt gctgccagcc ttctcagtta accgatgttt
     7321 tccgggctct tcgccatgct gacgcctgat aacgtattcc tggtcgtaaa acccgtcgat
     7381 atgcgccggg gtatcgacac actgacacag tacgtccaga acgaacttaa cgctgcatgg
     7441 catgacggtg ctgcttttgt cttcaccaat aaagtccgct cccgcataaa agttctccgg
     7501 tgggacaaac atggcgtatg gctgtgtacc cgacgactgc accggggaag cttccgctgg
     7561 ccccggaaag gtgatgccac atggcatctc acacaggatg agtttcactg gctggtcttt
     7621 ggggttgact ggcaacaggt gaaagggcat gacctggcaa aatgggtata ccagtaagaa
     7681 cccgaagatg ctcagagcaa cgcaataact caatgaaata taatgaatat ccatgcgtga
     7741 tgtcatataa tccaccgcat ggatatctcg cttctcagta ccaccagtga ccctgaacag
     7801 cttcgcgctc tggcaattgc catggtgcaa aaagtcatgg ctgagaatgc agaacttcag
     7861 aaccgtatcc gcatcctgga agagcagatg aagctcgcac gccagcagcg tttcggtaaa
     7921 aaatgcgaat cgctggccgg gatgcagcgt tccctgttcg aagaggatgt tgacgcggat
     7981 atcgcagaaa tctcggcgca tctggacaaa ctgcttcctc agaccgggga cgaagaaaaa
     8041 acaacaacac gtcctgttcg taagcccttg ccttcacctc ttccccgtgc tgaaaaggtt
     8101 atcccgccag ctgaggaacg ttgtcctgac tgtgatgccc ccctgcactt cattcgcgat
     8161 gaagtcagcg agaagctgga atatattccg gcacaggtcg tggtcaaccg ctatatccgc
     8221 ccgcagtaca gctgtccctg ctgtgagaaa gtgttcagcg gaaaaatgcc ggcacatatc
     8281 cttccgaaaa gtgctgttga gccatcggtc atcgcacagg ttgtcatcag caaatatacc
     8341 gaccatctgc ccctgtatcg ccagcagcac attttctccc gaatgggggt ggagctcccc
     8401 gtcagtacaa tggcggatat ggtcggtgtt gctggcgctg cactggcccc gctggcaaaa
     8461 ttgttacgcc acgagcttct gacacgggat gttattcatg cagatgaaac gtccctgagg
     8521 ctccttgata cccggaaagg cggtaaatcc tgctccggct ggctgtgggc ttacgtcagc
     8581 ggagaaagag taagcgtcaa cggagcaccg tattgacgct tatttattgg tgagtactac
     8641 gttccatggc aggagttcat caactcggtt ggaaggccat tctggcagta cgctcaggat
     8701 atggcgcaga tacgcttccg gatcgatacc gttcagacgg caggtgccga tcagcccgta
     8761 caacagtgct ccacgctcgc cgccgtgatc gctgccaaag aacataaagt ttttctttcc
     8821 gagacagact gcacgaagcg ctctttccgc agcattatta tccgcctccg ccagaccgtc
     8881 atcactgtaa taacagaggg catcccactg attcagtaca tagctgcacg cttcgcccag
     8941 tctggatttt ttcgacagcg taccattctt ctccaccatc cattcatgca gtgacgtcag
     9001 taacactttg cttcgctgct gcctgacggc aagacgctct gactccggta atccccgtat
     9061 ttcatcctcg atggcgtaca gttcactgat tcgcttcagg gcttcttctg ccgtcgcact
     9121 tttgctgctg atgtatacat cgtggatttt tcgccgggca tgggcccagc acgcaacttc
     9181 tgtcagtgca ccaccttcac gttctgcact gaacagccga tcgtaaccgc tgaacgcatc
     9241 cgcctgcagg ataccccgga agggacgaag gtgttgtacc ggatgttttc cctgcctgtc
     9301 tggtgagtag gcgaaccaga ccgccggtgg ctctgatgag cccgcattcc ggtcatcccg
     9361 gacatacgtc cagatgcgtc ctgtttttgc cttttttctg cccggtgcca gcacttttac
     9421 tggtgtgtcg tcagtgtgaa ccttgcgggt gttcatcacg taacggtaca gggtatcatt
     9481 cagcagagtc attaactgac aacatgcgtc aacccagttg gagagtaatg cccggctcag
     9541 ttcgatgccc tggcgggcaa agatttcact ctgacgatac agtggcaggt gttcgcagta
     9601 ttttcccgtt aacacgcggg caagtaatcc ggagcccgcg atgccgcgct ctatcgggcg
     9661 ggacggcgct ggcgcttcaa ctatacagtc acattttgta caggcttttt ttacccgaac
     9721 agtgcggatc actttcaggg cgctactcac cagttccagc tgctcagcac tgacttcccc
     9781 cagataatcc agctcactgc cacactccgg gcaacaactt tcttcaggct ccagacggtg
     9841 tatttcacgg ggaagatgtg ccggtaacgg acgacgatga cgtgactgtc gcaactggcg
     9901 gggaacctgc gggtcatcct cacgcccact gtaacgatcg ctttcctgtt cgcgttgttt
     9961 cagttgggcc tcagcctgtt caacctcacg ctgcagtttt tcagaacggg taccgaacaa
    10021 catccggcgc agtttttcta tctgggccct cagatgttct atttcccgct cctcctcttc
    10081 gatcttttcc tcggcacgtg ccagtgcaga gcgcaggaag gcctccgtct cttcaaccag
    10141 actcagttgc tgatctttct gacggagggc ttcagcctgc tcagagagca gcctttccag
    10201 ctcagtgata cgaatgaggt atttccgact catgaccgtt tttataatcc ggccatgaca
    10261 tttttacaac attgtcagtg cattaaggcg ggatgttttg ggctgacgcc agtccagttt
    10321 atcgaggagc attgccagct gcgagcgggt aatggatacc ttaccgtcac gcaccgcagg
    10381 ccagataaac tggccttcct ccagacgttt ggtgaacagg cacagaccat cagcatcagc
    10441 ccacagaatt ttaatcgtgt caccccgtcg gccgcgaaag ataaacaggt gaccggagaa
    10501 ggggttctca tccagcacat gttgtacctg ttctcccaga ccgttgaagg atttacgcat
    10561 atcagtaacg ccggcaacca gccagattcg agtgcctgat gggagcgaga tcatcgtccc
    10621 ctcccggtca gttcacggat caacaccgtg agcagctctg gtgaaggatt ttccagcgtc
    10681 atgttaccgt ggcggaactc aactttacag gaactggcac tgactgtgct ttgtgaagga
    10741 gtggataaaa gcggagtaag agccgccata ggctctttct gctcatcagg cgttatctca
    10801 acaggtaata attcaacgcc agcgccagaa gaggttgtta ccggaagacg ccgcgatata
    10861 cgcctttcgt tctgccagag cctgagccat ttgaacagga ggttatcatt gatatcgtgt
    10921 tcccgggcaa tacgggcaac agaggctcct ggttgtgaag ccagtttaac catttgaagt
    10981 ttaaactcat ttgaaaatga tctgcggggt tctgaggata atattttctg ttccataaca
    11041 ggtgtccact agttgaaaag gtgggcacct acgttaccaa tactggctta atggctacat
    11101 acggcggtca gtttacgctt acgagaaaga agcggtccgc cggtcgtctg ttttgactcg
    11161 cagaccggac gggccctcag atatcctgag gcctggctgc agggctggtg tggcggtacg
    11221 ctggtgagtg acggatacag cgtgtacaaa tctctggcgg ataatgaccc cggtattaca
    11281 tctgcctgtt gctggagcca cgcaaggcgc ggtttcgcaa atctgtataa ggccagcagg
    11341 gagccccgtg cagccatggc gctgagaaaa atcgcgggtc tttatcgcat tgagaagttc
    11401 atccgtgaac gtcctgtgga aaaaatacgg caatggcgac aacggtattc ccggccgata
    11461 gtgaatgacc tgtttgcctg gcctgaagag caggagccgt gctgtccgcc cgacggacca
    11521 ctaaacaaag cgattaatta tatcctgaac cggcgcgatg aactgagctg cttcctgagt
    11581 gacggagccg tgcccctgga caacaatatc tgcgagcgtg ctatccgccc ggtggtcatg
    11641 gggcggaaag catggttatt cgccggttca ctgatggccg ggaaccgcgc ggcacagata
    11701 atgagccttc tggaaacggc aaaacgtaat ggtctggagc cgcatgcctg gctgacagac
    11761 gttctgacgc gtctaccgga gtggccggaa gaccgactgg aagagttgct gccccttgag
    11821 ggatttacct tctccgggtg agtgatacct gccatcaggt gttcgtgcag cgggccataa
    11881 cccgcagtcg ggagttgaac gcctgacgac aggaaatgag ccagagcagc gaccacgcgg
    11941 gctgacagtg agtcagaacc tgaatcagcc cggagccatt cccggggctg tacttcatca
    12001 cctgccatca gtacccgtgt cagtacttcg cgacctgacg caattacccg actcagttct
    12061 tcagaacccg cctccgatac cagcctggcg gtcgcccctg tgcaacaaaa taaatcaacc
    12121 atcgtcagac ggtgatcaat cgtatgcgct gccagtggct cgcctgaaaa atgcgtcaat
    12181 atgagttcgc cggaacaata cctcagttct tcagaacccg cctccgatac cagcctggcg
    12241 gtcgccccgg cgcaacagaa taaatcaacc atcgtcagac tgcgatcatt cgtatgcatc
    12301 gctgatgatg cgcccgaaaa acgcgtcaat atgagttcgc cggaatatta ctgacgacac
    12361 ggtttgtagt tacgttcctt cggtgagtga gtacctgtgc gtcagggtaa cgcaatcagt
    12421 tcaggtttag cggggtgtca agtcggtttg gctaaccgac cgcagaatta actgtacctt
    12481 taaaggtcgc ccccgatata tctgcctgtc agacatatcg ccgattcggt atctcactac
    12541 ataaaactcc ttaaaaaaca atgcgattct gttggtacga agctttcagt aagtcagtcc
    12601 ggagaagcgc cagtacgata cagaatgagg ttgaaaaaaa cgttcacgtc tggtcacttc
    12661 ttcaaatgtt aaaaaacatc tcgtaaagag ccagtttatg cgggttcgtt gagtagacgt
    12721 gcgagcgtca ctttgtcagc tttaacgctg acgaaagtga cgcccgcaca gcttcaaact
    12781 ccacatgcac acgcaagcga ccaaatttca aatggtctta tgccgtgcgc acagtcataa
    12841 gaagcgcatg cggaaagtca gaaaatatct taagacgcag attttaaagg cgtttttatg
    12901 ccagtctgcc gggaatacac agtagtccgg ttgtccggaa gcaccacggt aatccgaagg
    12961 aaaaatcaac gtacctcaat caccgtagtg atatccgttc ccagaggcta aacggatgta
    13021 ccaccataaa ggcatcctct ttcaggctac gaggatattg caatcacccg aaggcgtttc
    13081 tctcagattg ctgaaacatt ggctggcagt cacccgaagg cgtccctctc agactgctgg
    13141 aacattggct ggtcgcaaaa gcgacagtta atgcaaatac aggttaacaa aaagggaaat
    13201 ctgccgtcaa cccaaccata tgaacatgtt gatttcatta tgaatgtgat gttctaatat
    13261 aaattaatca gtaacttcat aaaaagaata taagaaacag ataatgcatg
//
LOCUS       DBFIFI010000116        13276 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.116, whole genome shotgun sequence.
ACCESSION   DBFIFI010000116 DBFIFI010000000
VERSION     DBFIFI010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13276
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.116"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            45..536
                     /locus_tag="N7B39_003149"
     CDS             45..536
                     /locus_tag="N7B39_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HGU2423085.1"
                     /translation="MDHELKNLVLNINQLAALSGLHRQTVVARLKNIRPAGGHDKLKL
                     YRLTDILTEFMGLPPPVAEGEMDPHERKAWYQSERERLKFEQETAQLIPASDVRREFA
                     IWAKAVVQVLETLPDILERDCGLQPAAVSRVQSIIDDLRDQIALRVTEAGADDEEELQ
                     QEE"
     gene            536..2638
                     /locus_tag="N7B39_003150"
     CDS             536..2638
                     /locus_tag="N7B39_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HGU2423086.1"
                     /translation="MLNQETAKAARTDSGYILRAPRRMRVADAVAQYMRVPMGAGNSV
                     PWDPLVAPYVIEPMNCLASREYDAVIFVGPARTGKTIGLIDGWVIYNVICDPADMLII
                     QMTEEKAREHSKKRLARTFRVSPEVVSRLSPNKNDNNVYDRTFLAGNYLKIGWPSVNI
                     MSSSDYKCVALTDYDRFPEDIDGEGDAFSLASKRTTTFMSSGMTLVESSPGRDVKDVK
                     WRRTSPHEAPPTTGILSLYNRGDRRRWYWPCPHCGEYFQPCGDVVAGFRDIADPVLAS
                     EAAYIQCPSCSGRIMPEQKRELNGRGVWLRDGESINADGSRYGDPRRSRIASFWMEGP
                     AAAYQTLSQLVYKLLTAEQEYETTGSEETLKTVINTDWGLPYLPRASMEQRKSELLEQ
                     RAEPVPSSSVPDGVNFLVATVDVQAGRHRRFVVQVTGYGSRGERWIIDRYNITQSLRG
                     DSDGESQRIDPASYPEDWDVLLTDVFHKSWPLASDPSQQMRLMAMAVDSGGEDGVTDN
                     AYKFWRRCRRDGLGKRIYLFKGDSIRRAKLITRTFPDNTGRTGRRAQAAGDVPLWLLQ
                     TDALKDRVNNALWRDSPGPGYVHFPDWLGSWFYDELTYEERSSDGKWSKPGRGANEAF
                     DLMVYAEALVILHGYEKIRWPDAPGWASRETWLESVPDSTEPSPSPEPVSTPVKKQKR
                     KKTVTDDVNPWLTSGGWL"
     gene            2635..2847
                     /locus_tag="N7B39_003151"
     CDS             2635..2847
                     /locus_tag="N7B39_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2423087.1"
                     /translation="MNQNDIEAMIQRYTEAEMAVLDGKSVTFNGQQMTMENLSEIRQG
                     RQEWERRLAALITRRRGHPGYRLARF"
     gene            2775..3899
                     /locus_tag="N7B39_003152"
     CDS             2775..3899
                     /locus_tag="N7B39_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU2423088.1"
                     /translation="MGAPPCGSDYTTTGASRVPAGEVLMAILDDVIGVFSPGWKAARL
                     RSRAVIQAYEAVKTTRTHKARRENRTADQLSQYGAVSLREQARYLDNNHDLVIGVFDK
                     LEERVVGKNGIIVEPHPVLRNGAIARDLAAEIRTRWSEWSVSPEVTGQFTRPMLERLM
                     LRTWLRDGEVFAQMVSGRINSLTPSAGVHFWLEALEPDFIPMTSDESNRLNQGVFVDD
                     WGRPEKYLVYKSRPVSGRQMETKEVDAERMLHLKFVRRLHQMRGTSLLSGVLIRLSAL
                     KEYEDSELTAARIAAALGMYIRKGDGQSYEPDGNGSKDKERELTIQPGIIYDDLKPGE
                     EIGMVKSDRPNPNLETFRNGQLRAVAAGSRLNFFQYSAQL"
     gene            4021..4356
                     /locus_tag="N7B39_003153"
     CDS             4021..4356
                     /locus_tag="N7B39_003153"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU2423089.1"
                     /translation="MASGVIRLPRDLDRSSLYTAVYSGPVMPWIDPVKEAEAWKIQIR
                     GGAATESDWVRAGGRNPDDVKRRRKAEIDENRKLDLVFDTDPASDKGGSSAATKRQEP
                     QHTDDQSEE"
     gene            4301..6328
                     /locus_tag="N7B39_003154"
     CDS             4301..6328
                     /locus_tag="N7B39_003154"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HGU2423090.1"
                     /translation="MPQRNDRSRSTPTTSPKNNSWFRMQAGHQSDADIYIYDEIGFWG
                     VTAKQFISDLNALGDITHINLNINSPGGDVFEGIAIFNALKTHGASITVYVDGVAASM
                     ASVIAMVGKPVIMPENSFMMIHKPFGFTGGDAEDMRTYADLLDKVEAVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAHGFADQVTPAVKAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRVQDDGPAASRTPVQAAAPVVDENSIRAQVLAEQKARVNGINDLFAMFG
                     GRYQTLQAQCLADPECSLEQAREKLLNEMGRESTPSNKNTPAHIYAGNGNFVGDGIRQ
                     ALMARAGFEKTERDNVYNGMTLREYARMSLTERGIGVSSYNPMQMVGAAFTHSTSDFG
                     NILLDVANKAILQGWEDAPETYEQWTRKGQLSDFKIAHRVGMGGFSALRQVREGAEYK
                     YVTTGDKQATIALATYGELFSITRQAIINDDLNMLTDVPMKLGRAAKSTIADLVYAIL
                     TSNPKISTDNVSLFDKAKHANVLESAAMDVASLDKARQLMRVQKEGERHLNIRPAFVL
                     VPTAMESVANQVIRSSSVKGADINAGIINPVKDFATVIAEPRLDDNSQTTFYLAASKG
                     SDTIEVAYLNGVDTPYIDQMEGFSVDGVTTKVRIDAGVAPVDHRGLVKCTA"
     gene            6415..6738
                     /locus_tag="N7B39_003155"
     CDS             6415..6738
                     /locus_tag="N7B39_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HGU2423091.1"
                     /translation="MAKNFVEEGKTVAIVASAAISSGDLVQVGDVFAVALTDIPQGET
                     GDGMTEGVFMLPKLKTDDMKTGKKVYLKSGKVQLTNSGSDPLVGVVWADAGTSAEEVP
                     VKLNV"
     gene            6731..7006
                     /locus_tag="N7B39_003156"
     CDS             6731..7006
                     /locus_tag="N7B39_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HGU2423092.1"
                     /translation="MSDPFSRLAARMDAITVRKMGKTASINDVDMTVIPGETLAELNA
                     LSGPAVSLVVFSSGYRPRRGDRVVYDGQQWTVTRHERFNGKPMIFIE"
     gene            7018..7596
                     /locus_tag="N7B39_003157"
     CDS             7018..7596
                     /locus_tag="N7B39_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU2423093.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAGSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLGTARVRLAQR
                     GGKLQYRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIPLSGPLTQ
                     AFEDARDRIIAAEMPKQLGYALKQQLRLWLTR"
     gene            7593..7994
                     /locus_tag="N7B39_003158"
     CDS             7593..7994
                     /locus_tag="N7B39_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HGU2423094.1"
                     /translation="MNRHTQIRQVVLARLREQCGDSATFFDGLPAFVDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDVSALSGLID
                     TLIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            8005..8748
                     /locus_tag="N7B39_003159"
     CDS             8005..8748
                     /locus_tag="N7B39_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU2423095.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASASVNVGATTTLTFTVKPDGASDKAISVHSTDPQTATVTLNGLVATVKG
                     VKQGSVSIVGMTADGNFVAVAAVTVSAAG"
     gene            8809..9195
                     /locus_tag="N7B39_003160"
     CDS             8809..9195
                     /locus_tag="N7B39_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HGU2423096.1"
                     /translation="MFLKQGTFNYEKQSVVLSELSGLQRIEYLAFVQQRTAKFDAEEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASVITTWSYDALGAGAEM
                     VLSLSGMGAIENAGDLEHEVLTPEKS"
     gene            9204..9533
                     /locus_tag="N7B39_003161"
     CDS             9204..9533
                     /locus_tag="N7B39_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HGU2423097.1"
                     /translation="MQFVMRLAREFRRADWRRMLSEMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            9505..12570
                     /locus_tag="N7B39_003162"
     CDS             9505..12570
                     /locus_tag="N7B39_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU2423098.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASVHAQTVEKSAQAHERMAREVEQTRQRMEALSQK
                     MREEQAQAMALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQIRQARNSGGIGQQ
                     DYLALISEVTAKTRVLTQAEEEATRQKVAFIRQLKEQATRQNLSSSELLRAKAAQLGV
                     SSAAEVYIRKMEQAGKATHSLGLKSAAARQEIGVLIGELARGNLGALRGSGITLANRA
                     GWIDTLMSPKGMMPGAVIGGIAAAVYGLGKAWYDGQKEGEEFNRQLSLTGHYAGVTAG
                     QLWTLSRAISGNGITQHAAAGALAQVVGSGAFRGNDIGMVARAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNELHFLTATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     RRTADIDNNLNALGSTLKYLSDLWSRFWDAAMNIGREDSLDEQIAALQEKVSRAKRLP
                     WTASSSQVEYDQQRLNDLQEKKRQKDLQDAKEQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKALASGKKKTRETRNDEATRLLL
                     QYSQQQAQVEGQIAAARQSAGIATERMTEARKQLLALQQRISDLDGKKLTADEKSVLA
                     RKDELIQALTLLDVKQQELQKQTALNELKKKTIQLTSQLAEEERAQRQQHDLDIATVG
                     MGDQQRQRYQVQLSLRQKYQQQLEQLRRDSEQKGTYNTDDYRKAEQALTESLNRQLNE
                     NRRYWQQLEVVQGNWKNGVLRAFQDFTVDADNTAETAEQVFSSAFSNMGNGLATFVTT
                     GKLNFKSFTSSVLSDMAKILAQATMMKSIKGIGSVLGFDLSSLSLNANGGIYQSADLS
                     RYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADIGGSGMAMFSPQ
                     YNIEINNDGTNGQIGPAALKAVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            12570..12899
                     /locus_tag="N7B39_003163"
     CDS             12570..12899
                     /locus_tag="N7B39_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU2423099.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKCEHPSLKAFLERHGGVRAFQWTPPYDWKPIRVVCRKWSASVGALWVTITA
                     DFEQVVA"
     gene            12909..>13276
                     /locus_tag="N7B39_003164"
     CDS             12909..>13276
                     /locus_tag="N7B39_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HGU2423100.1"
                     /translation="MQDIPQETHHETTRLTQSAQAVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGTGFELNGKGSSARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGN"
BASE COUNT         3235 a         3076 c         4050 g         2915 t
ORIGIN      
        1 aaagttgttc gcacgatgca tgtttaaagc tctccggagg aaatatggat catgagttga
       61 aaaacctggt gctgaatatt aatcaactgg cggctttatc tggtctgcac cgccagactg
      121 tcgtggcaag actgaaaaac attcgtcccg ctggtggaca tgacaaactc aagctatacc
      181 ggttgaccga tattctgact gaatttatgg ggttaccacc gccggttgct gagggcgaaa
      241 tggatccaca tgaacgcaaa gcctggtatc agtctgaacg tgagcgtctt aagttcgaac
      301 aggaaacggc acaactcatt ccggccagtg atgtcagacg ggagtttgcc atctgggcaa
      361 aagcggtcgt gcaggtgctg gagacattac cggatattct ggaacgtgac tgtggtctgc
      421 agcctgccgc tgtgagccgt gttcagtcca ttattgatga tctgcgcgat cagatagccc
      481 tgcgggtgac cgaagcaggt gcggatgatg aggaggaatt acagcaggag gagtaatgct
      541 gaatcaggaa accgcaaagg cagcacgaac cgattcaggt tatatccttc gcgcaccgag
      601 acgaatgcgg gttgctgatg ccgttgctca gtatatgcgg gtgcccatgg gggccgggaa
      661 ctcagtcccg tgggatccgc tggtggcacc gtatgttatt gagccgatga actgcctggc
      721 ctcgcgtgaa tacgacgcag tgatatttgt tggcccggca cgaaccggca agactatcgg
      781 cctgattgac ggctgggtga tttacaacgt gatttgcgat cctgcggata tgctgatcat
      841 tcagatgacg gaggaaaaag cccgcgaaca ctccaaaaaa cgactcgcca gaacgtttcg
      901 cgtcagcccg gaagtggtca gtcgcctgag tccgaacaaa aatgacaaca acgtttatga
      961 cagaacattc cttgctggca actacctgaa aatcggctgg ccgtcagtca atatcatgtc
     1021 ctcatcagat tataaatgcg tggcgctgac ggattatgac cgttttccgg aagatattga
     1081 tggcgagggg gatgccttct ctcttgcctc aaaacgtacc accacattta tgtccagtgg
     1141 tatgacgctg gtggagagtt cccccggcag ggatgtgaag gatgtgaaat ggcgacggac
     1201 ttcaccgcat gaggctccac caaccacggg gatactgtcg ctctataacc gtggcgatcg
     1261 ccgtcgctgg tactggccct gtccacactg tggtgagtat tttcagccct gcggcgatgt
     1321 ggttgctggt ttccgtgata ttgccgatcc cgtgctggca agtgaggcgg cttatattca
     1381 gtgtccttcc tgttcaggac ggattatgcc tgaacaaaaa cgtgagctga acggacgtgg
     1441 ggtctggttg cgggatggtg aatccatcaa tgcggatggt agtcgttatg gtgatccccg
     1501 acgctcacgt attgcgtcat tctggatgga gggtccggca gctgcttacc agacactctc
     1561 gcaactcgtt tacaaactgc ttactgcaga acaggaatac gagacaaccg gaagtgaaga
     1621 aacactcaag acggttatca ataccgactg gggattacct tatcttcccc gcgccagcat
     1681 ggagcaacga aaaagtgaac tgcttgagca gcgggcagag ccagttcctt ccagcagtgt
     1741 gccggatggc gttaatttcc ttgtggcgac agtggatgtg caggcgggac gtcatcgccg
     1801 ttttgtggtt caggtaacgg gctatggcag ccgtggcgaa cgctggatta ttgatcgtta
     1861 caacatcacg cagtcattgc gcggtgacag cgacggtgag agccagcgaa ttgatccggc
     1921 cagctatccg gaagactggg atgtcctgct gacggatgtt tttcataaaa gctggccgct
     1981 ggcctccgat ccttctcaac aaatgcgact gatggcaatg gcggtggact ccggcggtga
     2041 agacggggtc actgataatg cctataaatt ctggcgtcgt tgccgtcgtg atggccttgg
     2101 taaacgtatt tacctgttta agggcgacag catccggcgc gcaaaactga tcacccgtac
     2161 attccctgat aacaccggac gaacgggccg acgggcgcag gccgcaggtg atgtgccgct
     2221 ctggcttctt cagacggatg cactgaaaga ccgggtgaat aacgcgttat ggcgtgactc
     2281 tccaggtccc ggctatgtgc atttccctga ctggctgggg agctggtttt acgacgaact
     2341 gacgtatgaa gagcggagta gtgacgggaa atggagtaag ccgggtcgcg gtgccaacga
     2401 agcttttgac ctgatggtgt atgccgaggc tctggtcatt ctgcatggat acgaaaagat
     2461 ccgctggccg gatgcaccgg ggtgggcgag ccgggaaacc tggctggaga gtgtcccgga
     2521 cagtaccgaa ccgtcaccct caccggaacc ggtatccacg cctgttaaaa aacaaaaacg
     2581 gaagaaaaca gtaactgacg atgttaaccc ctggctgact tccggaggat ggttatgaac
     2641 cagaatgata tcgaagccat gattcagcgt tatacggaag ctgaaatggc ggtgctggac
     2701 ggaaaatccg tcacctttaa tggtcagcag atgaccatgg aaaacttatc tgagatccgg
     2761 cagggacggc aggagtggga gcgccgcctt gcggctctga ttacacgacg acgggggcat
     2821 cccgggtacc ggctggcgag gttctgatgg caattcttga tgatgtgatt ggcgtttttt
     2881 caccaggatg gaaagcggca aggctgcgtt cccgtgcggt gatccaggct tatgaggccg
     2941 taaaaacgac gcggacacac aaagcccggc gggaaaaccg aactgccgac cagttaagcc
     3001 agtacggggc cgtgtcgtta cgtgagcagg cccgttacct tgataacaac cacgatctgg
     3061 ttattggtgt atttgacaag ctggaagaac gggtggtggg gaaaaacggg attattgtcg
     3121 agccacatcc ggtattacgc aatggggcca ttgcccgtga tctggcagcg gagatacgca
     3181 cccgatggag tgaatggtct gtcagtccgg aagtcaccgg gcagtttacc cgtccgatgc
     3241 tggaacgtct gatgctgcgt acctggctgc gcgatggtga ggtgtttgcc cagatggttt
     3301 ccgggcgcat aaacagcctg acgccttctg ccggtgttca tttctggctg gaggcgctcg
     3361 agccagactt tattcccatg accagtgatg agagcaacag gctgaatcag ggcgtgtttg
     3421 ttgatgactg ggggcgtccc gaaaaatatc tggtgtataa aagccgtccc gtatccggac
     3481 ggcagatgga aaccaaagaa gtggatgcag agcgaatgct gcatcttaaa tttgttcgcc
     3541 gtctgcacca gatgcgcggg acgtctttgt tgtccggtgt gctgatccgc ctcagtgccc
     3601 tgaaagagta tgaggattct gagctgactg cagcaaggat cgccgctgct ctggggatgt
     3661 acatccggaa aggcgatggg cagagctatg aaccggatgg taatggcagc aaggataagg
     3721 aacgcgagct taccattcag ccaggcatta tttacgacga tctgaaaccc ggcgaagaaa
     3781 tcggaatggt gaagtcggat cgccccaatc ctaaccttga aacttttcgt aatggtcagt
     3841 tgcgtgccgt ggcggcgggc agtcgtctga attttttcca gtacagcgcg caactataac
     3901 ggcacttaca gcgcccagcg tcaggagctg gttgaatcca ctgatggcta cctgatcctg
     3961 caggactggt ttattggtgc cgtcacccgc ccgatgtatc gtgcctggct gaaacaggct
     4021 gtggcatccg gtgttatcag gctaccccgc gatcttgacc gttcttcact gtataccgcg
     4081 gtgtattccg gaccggtgat gccgtggatt gaccctgtta aggaggctga ggcctggaaa
     4141 attcagattc gtggtggagc agcgacagaa tcagactggg tacgtgcagg tggtcgtaat
     4201 ccggatgatg tcaaacgtcg gcgcaaggcc gaaattgatg aaaaccgcaa gctggatctg
     4261 gtatttgata ccgatccggc cagtgataaa ggaggcagca gtgccgcaac gaaacgacag
     4321 gagccgcagc acaccgacga ccagtccgaa gaataattcc tggttcagga tgcaggctgg
     4381 tcaccagagt gacgcggata tttatattta tgacgagatt ggtttctggg gtgttacagc
     4441 gaagcagttt atcagtgatc tgaatgcact gggcgatatc acccacatta atctcaatat
     4501 caattcaccg ggtggcgatg tctttgaagg catcgccatt tttaatgcac tgaaaacaca
     4561 tggtgcgtcc attaccgttt atgtcgacgg tgtggcggcg tcaatggcgt cggtcattgc
     4621 gatggtggga aaaccggtca ttatgccgga aaactccttc atgatgattc ataaaccatt
     4681 tggctttacg ggcggtgatg cggaggacat gcgcacctat gccgacctgc tcgataaagt
     4741 tgaggcggtt ctgttacccg cttatgcaca gaaaaccggg aaaaccaccg atgaaattgc
     4801 tgccatgctg gcggatgaga cctggatgtc cggtgccgaa tgtctggcac atggatttgc
     4861 tgatcaggtg acgccagccg ttaaggcaat ggcatgtatt cagtcaaaac gtacagagga
     4921 atttaaaaag atgccggaat ccattcgaaa catgattact ccgccacgca acagtgctcc
     4981 acgcgtacag gatgatggac ctgcagcctc ccggacgcca gtgcaggcag cagcacctgt
     5041 ggtggatgaa aacagtatcc gtgcgcaggt actggcagag caaaaagcgc gtgtaaacgg
     5101 tattaatgat ctgtttgcca tgtttggcgg gcgttatcag acgctgcagg ctcagtgtct
     5161 tgccgatcct gaatgttcgc tggagcaggc ccgcgaaaag ctgttgaacg agatggggcg
     5221 cgagtccacg ccatctaata aaaatacccc ggctcatatt tatgccggaa acggtaattt
     5281 tgtgggggat gggatccgcc aggcgctgat ggcgcgtgcc ggatttgaaa aaaccgaacg
     5341 tgataatgtc tacaacggga tgaccctgcg tgaatatgcc cgtatgtcac tgactgaacg
     5401 gggtattggg gtttccagtt ataacccgat gcagatggtc ggtgcggcgt tcacacacag
     5461 tacgtctgac ttcggtaata ttctgctgga tgttgcgaac aaagccattc tgcagggctg
     5521 ggaagatgcc cctgaaacct atgaacagtg gacgcggaaa ggtcagttgt ctgattttaa
     5581 aattgcccat cgtgtgggta tggggggctt cagtgctctg cgtcaggtgc gtgaaggggc
     5641 ggaatataaa tacgtcacca ccggagataa acaggccact attgcactgg cgacctatgg
     5701 cgagctgttc agtatcaccc gtcaggccat tatcaatgat gatctgaata tgctgaccga
     5761 tgtcccgatg aaactgggcc gtgcggcgaa atccactatt gccgatctgg tttatgccat
     5821 tctgacgtct aacccgaaaa tctccacaga taatgtaagt ctgttcgata aagcgaaaca
     5881 tgcaaacgta ctggagagcg ctgcaatgga cgtggcatcg ctggataaag cccgccagtt
     5941 gatgcgcgtt cagaaagagg gggagcgtca tctgaatatt cgtcctgcgt tcgtactggt
     6001 accgacggcg atggagtctg ttgctaacca ggtcattcgc tcctcaagtg tcaagggggc
     6061 tgacattaac gccggtatta ttaacccggt gaaagatttt gcgaccgtta ttgcagagcc
     6121 tcgtcttgat gataacagcc agaccacctt ctacctggct gcgtcaaaag gctccgatac
     6181 gattgaagtg gcttatctca acggtgtgga tacgccatat attgatcaga tggagggctt
     6241 cagtgtggat ggcgtgacaa cgaaagtgcg tattgacgcc ggtgtcgcgc cagttgatca
     6301 ccgcggtctg gtgaaatgta cggcgtaaac gtcgcagaca acaactctga tggcccgtaa
     6361 gggctttttt tgtacctgaa atcagcccct gaacggggct gtgcggagac agttatggca
     6421 aagaattttg tagaagaagg aaaaacggtg gcgattgttg ccagtgcagc catcagcagc
     6481 ggagatctgg tgcaggtggg cgatgttttt gcggtggcgc tgaccgatat tccacagggt
     6541 gaaacaggcg acggcatgac cgaaggtgtg tttatgctgc ctaagctgaa aacggatgac
     6601 atgaaaacgg gtaagaaggt ttatctgaag tcaggaaaag ttcagctgac taacagcggc
     6661 tctgatccgc tggtcggggt tgtctgggca gatgccggaa ccagtgcaga agaagtgccg
     6721 gtaaaactca atgtctgatc ccttttcccg gctggcagcg cgtatggatg ctatcacggt
     6781 cagaaagatg ggaaagacag cctcgattaa tgatgtcgat atgactgtga tcccgggaga
     6841 aacactggca gagctgaatg ctctgtccgg acctgcggtc tctctggtgg tgttttcttc
     6901 gggataccgc ccacggcgcg gggatcgcgt tgtttatgac ggacaacaat ggacggtcac
     6961 acggcatgaa cgctttaacg gtaagccaat gatctttatt gagtaaagag gtgtgggatg
     7021 aaggggcttg agaatgccat ccgcaatctg aacagccttg atacccgtat ggtgccacag
     7081 gccagcgcat gggcgataaa ccgtgtggca cagaaagcgg gctcggttgc cacccggcag
     7141 gttgccggga ataccgttgc gggagataac caggtgaaag ggatccccct gaaactggtt
     7201 cgtcagcgtg tccgggtgtt taaagccagt ccgtcaggaa aaatgacggc caggatccgc
     7261 gttaaccggg gcaatctgcc cgccattaag ctggggacag cccgggtcag actggcccag
     7321 cgtggtggaa aactgcagta ccgtggcagt gtgctgaagg tgggtaaata tcttttccgg
     7381 gatgcgttta ttcagcaact ggcgaatggt cgctggcatg tgatgcggcg tattgatggc
     7441 aaaaatcgtt accccattga tgtggtgaaa atcccgctgt ccggaccgct gacacaggca
     7501 tttgaagatg cccgcgaccg catcattgct gcggaaatgc cgaaacagct ggggtatgca
     7561 ctgaaacaac aactgaggtt atggctgacc cgatgaaccg acatacacaa atccgccagg
     7621 tcgtactggc acgccttcgg gaacagtgtg gagacagcgc cacgtttttt gacgggcttc
     7681 cggcatttgt tgatgcgcag gaactgcctg ccgtggcggt gtggctgagt gatgctcagt
     7741 acaccggaaa aatgacggat gaagatgact ggcaggctgt tctgcatatt gctgtcttca
     7801 tccgggcaca ggcaccggat tcagagctgg atatgtggat ggagagcacc attttcccgg
     7861 ccctgaatga tgtatcggca ctttccggac tcatcgacac cctgatccca ctcggtttta
     7921 actatcaacg tgataatgag atggccacct gggcgatggc ggaaatcacg taccagatca
     7981 cgtacacgaa ttaaggaggt ggtaatgacc acgccaaatc cactggcaaa gacgaaaggt
     8041 gcggggacga cgttctggat gtataccggc aacggcgatg cgtttgcgaa ccctttgtcg
     8101 gacactgact ggctgcgtct tgcgatggtg aaggatctgc aacctggcga aatgaccgct
     8161 gatgcagaag atgacactta tctcgatgat gaagatgcag actggaaaac gacaacccag
     8221 gggcagaaat ccgttggtga tacttcggcg acgctggcct ggcgtccggg tgacagcggg
     8281 cagaaaaaac tggttcagtt gttcgactcc ggtgaagtct gcgcgtttcg tatcaaatat
     8341 cccaacggca ctgttgatgt tttccgcggc tggctgagct cactgggtaa aaccattgcc
     8401 tcaaaagacg tgatgacccg cacagtgaaa atcagcggtg tggggcgtcc atatctggca
     8461 gaggagggca ctgaaactgt gggcgttacc gggctgacgg tggcaccggc atccgccagt
     8521 gtcaatgtgg gagcaaccac cacgctgacc tttacagtaa aacctgacgg agccagtgac
     8581 aaagcgatca gtgtgcattc gacagatcca cagacagcca ctgtgaccct gaacggactt
     8641 gtggccacgg tgaaaggcgt gaagcagggc agcgtcagca ttgtgggcat gaccgctgac
     8701 gggaattttg tggctgtggc tgcggtgact gtcagcgctg caggttaaca ggacgatact
     8761 catcatttgc cccggttatc cggggctttt ttgcaggtgg agaacatgat gtttctgaaa
     8821 cagggcacgt ttaattatga aaagcagtcc gtggtgctca gtgagctgtc cgggctgcag
     8881 agaattgaat atctggcgtt tgttcagcag cgaacggcaa agtttgatgc cgaagaggga
     8941 gaactgccgg aggctgaacg acagattgct tttctgcgga tggggatgga tatcaatgcc
     9001 tggctggttt cccgctcact gtggaatgcg gaacagtctc aggatgttga gacgctttgc
     9061 gcatccgtta ttacaacatg gtcgtatgat gccctgggag cgggggcgga gatggttctg
     9121 tcgctgagcg gtatgggagc cattgagaat gccggggatt tggagcatga ggtgctgacg
     9181 ccggaaaagt cctgacgcgg gaaatgcagt ttgtcatgcg gcttgcccgg gagttccggc
     9241 gggcagactg gcggcggatg ctgtcggaaa tgtcggccac tgagcttggt gagtggggcg
     9301 attatttccg gatgcagagc ttcagtgatg tgtggatgga tgcgcagttt gcctcgctga
     9361 aggcattgat cgtgagaatg gtgtccggta gcagtgatgc tgcggtggct gatttcagcc
     9421 ttttaccgga agagaacggg ataccggagc gaacggacga agaactgatg catcttgggg
     9481 aaggtatttc cggaggtgtg cgttatggac cagatagcca acctggtcat tgatttgggg
     9541 attgatgcgg cagagtttaa aaatgaaatt ccccgtatca aaaaccttct gaatggtgca
     9601 gccagcgatg cagaacggtc ttctgcccgt atgcagcgtt ttatggagcg tcagactcag
     9661 gccgcccggc agacaatgca ggcggcttct tcggctgcaa cagccgcatc cgtccatgcg
     9721 cagacggtgg agaagagcgc acaggctcat gaacgcatgg cccgcgaggt ggagcaaacc
     9781 cgccagcgta tggaggcact gagccagaaa atgcgcgagg aacaggcgca ggccatggct
     9841 ctggcggagg ctcaggataa agcggctgct gcgttttatc gtcagattga cagtgtgaaa
     9901 caggccggtg cggggctgca ggaattacag cgtattcagc aacagatccg acaggccaga
     9961 aacagtggcg ggattggtca gcaggattat ctggcgctga tttctgaggt tacggcgaaa
    10021 acccgtgttc ttacacaggc tgaggaagag gctacccgac agaaagtggc gtttatccgt
    10081 cagcttaaag agcaggcaac ccgccagaat ctttcttctt ctgagttgct tcgtgctaag
    10141 gctgcccagc tgggggtaag cagtgctgca gaagtgtata tccgcaaaat ggagcaggca
    10201 ggaaaagcca cgcattcgct gggtctgaaa agtgcagcag cccgtcagga gataggcgtt
    10261 ctgataggtg aactggcccg cggcaattta ggtgcgctga ggggatccgg aataacgctg
    10321 gctaaccgtg ccgggtggat agacacactg atgtcaccga aaggcatgat gccaggagcg
    10381 gttattggcg gtattgccgc ggctgtctat ggtctgggta aagcctggta tgacggtcag
    10441 aaggaggggg aagaatttaa ccgccagctg tcgctgacgg ggcattatgc cggagtcact
    10501 gccgggcagc tgtggacgct cagtcgtgct atttccggga atggtatcac gcaacatgct
    10561 gcagccggtg cgctggctca ggtggtgggg agtggtgcat ttcgtggaaa cgatatcggt
    10621 atggtggcga gagctgccgc acagatggag cgatcggttg gccagtcggt cagcgatacc
    10681 ataaatcagt ttaagcggct gaaggatgat cctgtaaatg ccgcgaaggc tctggacaat
    10741 gagctgcatt ttcttactgc cactcagctt gagcagatac gcgtccttgg ggagcagggg
    10801 cggtccagtg atgctgcacg gatagccatg tctgcactgg cagaggaaac cggtcggcgt
    10861 actgcggata ttgataataa cctcaatgcg cttggcagta cgctgaagta tctgtctgat
    10921 ttgtggagtc gtttctggga tgcggccatg aatattggtc gtgaagactc gctggatgaa
    10981 cagattgccg ctttacagga gaaagtgtcg cgggcgaaaa gactcccctg gacggcatca
    11041 tcttctcagg ttgaatacga tcagcagcgt cttaacgatc ttcaggagaa aaaacgccag
    11101 aaggatttgc aggatgcaaa agagcaggca gagcggaatt atcaggagca acagaaacgc
    11161 cgtaatgctg aaaatgctgc actgaaccgg atgaatgaaa cggaagcagc acgacatcag
    11221 cgtgaaatag cgcgtattaa tgccatgcag tacgccgatc aggctgtcag ggatgcggcg
    11281 atacaacgtg aaaatgaacg ttacgagaaa gccctggcat ccggtaagaa aaaaacacgc
    11341 gaaacccgta atgatgaggc cacccggtta ttgctgcagt acagtcagca acaggcacag
    11401 gtggaaggac agattgctgc tgccagacag tcagcaggca ttgccacgga aaggatgaca
    11461 gaagcgcgta aacagcttct ggctctgcag cagcgcatca gcgacctgga cgggaaaaaa
    11521 ctgacggcag atgaaaagag tgtgctggcc cgtaaagatg aactgattca ggcactgacg
    11581 ctgctggatg taaaacagca ggagcttcag aaacagacgg cactcaacga gctgaagaaa
    11641 aaaacaattc agctgaccag tcaactggct gaagaagagc gcgctcagcg tcagcaacat
    11701 gacctggata tcgccacggt gggtatgggt gatcagcagc gacagcgata tcaggtacaa
    11761 ctgagtcttc gccagaaata ccagcaacag ctggagcagt tgaggcggga tagtgagcag
    11821 aaagggacat ataacacgga tgactacaga aaggccgagc aggcgctgac ggagagcctg
    11881 aaccgacaac tgaatgagaa tcgccgttac tggcaacagc ttgaagttgt acagggtaac
    11941 tggaaaaacg gagtcctgcg tgcatttcag gattttaccg tggatgcaga taatacggca
    12001 gaaacagcag aacaggtgtt ctcgtcagcc ttcagcaaca tgggaaatgg cctggcaact
    12061 tttgtcacta ccggcaaact caatttcaaa tccttcacct cttctgtgct gtcagatatg
    12121 gcgaaaatcc tggcgcaggc aaccatgatg aaatcgataa aagggattgg cagtgtactg
    12181 ggatttgatc tcagcagcct ttccctgaat gccaatgggg ggatttatca gtctgctgat
    12241 ttgagtcgtt acagtggcac ggtggttaac cgtccgacgt tttttgcttt tgcaaaaggc
    12301 gcgggtgtga tgggggaagc gggacctgaa gccattctgc cattgcgtcg tggtgctgac
    12361 ggtaagctgg gggttgtggc ggatattggg ggttcaggta tggcgatgtt ttccccgcag
    12421 tacaacatcg agatcaataa cgatggcacg aacgggcaga taggtccggc tgccctgaag
    12481 gcggtttatg acctcgggaa aaaagcggca gcggacttta tgcaacagca ggcccgtgat
    12541 ggtggtcggt taagtggagc atatcggtaa tggagacgtt tcactggaaa gtgcgcccgg
    12601 atatgaatgt ggtatcagag ccgaaagtgg tgacagtgaa gctgggcgat ggttatgaac
    12661 agcgtcgtgc ggcgggactg aataaccagt tgtcgactta cagcgtgacg atacgtgttc
    12721 gtaaatgtga acacccatct ttaaaagcct ttctggaacg gcacggtggc gtccgtgcat
    12781 ttcagtggac gccaccttat gactggaaac cgatcagggt ggtttgtcgt aaatggtcgg
    12841 caagcgtggg ggcgctgtgg gtaaccataa cggcagattt tgaacaggtc gtggcatagg
    12901 aggctctgat gcaggatatt ccacaggaaa cacatcatga gacgacacgc ctcactcagt
    12961 cagcccaggc ggtgctctgg gaaatcgatc tgacagaggt cggtggtgaa cgttattttt
    13021 tctgtaatga gcagaacgaa aaaggtgagc cggttacctg gcaggggcgg cagtatcagg
    13081 cataccccat tcaggggacg ggatttgaac tgaacggcaa gggcagttct gcccgtccga
    13141 cactgacggt ttctaacctg cacggcatgg tcacggggat ggcggaagac ctgcagagtc
    13201 tggtcggcgg aacggtggtc cggcgtaagg tttacgcccg ttttctggat gcggtgaact
    13261 tcgtcaacgg aaacag
//
LOCUS       DBFIFI010000117        13269 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.117, whole genome shotgun sequence.
ACCESSION   DBFIFI010000117 DBFIFI010000000
VERSION     DBFIFI010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13269
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.117"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..436
                     /locus_tag="N7B39_003165"
     CDS             <1..436
                     /locus_tag="N7B39_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HGU2423101.1"
                     /translation="ILFDYAWFDNTIFIKTLFKNTCFYNVRAKNVYLEGAYLNNDNIV
                     NQANNSTEKQSIDSTDKQANDSTVQQSIDSTVQQANDSTDKQANDNIDKQVNDSTDKQ
                     AKNSTEQQDSNSFNQARLKKEVNRRFSIPGLTSYQPTYIVEE"
     gene            702..1544
                     /locus_tag="N7B39_003166"
     CDS             702..1544
                     /locus_tag="N7B39_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313321.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HGU2423102.1"
                     /translation="MKKRITFIVFLCAIVAASLFFVQSCVRKSQHVAGFQNYQATIDG
                     KEITGVTKNISSLTWSAQSNTLFSTINKPATIVEMTTEGDLIRTIPLDFVKDLETIEY
                     IGDNKFVISDERDYAIYVISLNADSEVSILKKIKIPLQETPTNCGFEGLAYSSQDHTF
                     WFFKEKNPIEVYKVTGLLRSDELHISKDKTLQRQFTLDDVSGAEFNPQKNTLLVLSHE
                     SRALQEVTVRGDVIGEMSLTKGKYGLSHNIKQAEGIAMDDSGNIYIVGEPNLFYRFTS
                     TKSR"
     gene            1612..2790
                     /gene="yjiJ"
                     /locus_tag="N7B39_003167"
     CDS             1612..2790
                     /gene="yjiJ"
                     /locus_tag="N7B39_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2423103.1"
                     /translation="MNSTSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMLAE
                     GEFSFSELSWIASGNYAGYLAGSLLFSFGAFHLPSRLRPFLLASALATGLLILAMAWL
                     PPFLLVFIIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGVGIALGNEYVLA
                     GLHFALSSQTLWQGAGALSAIILLALALLIPSNKHVIPPAPLAKIAQQPMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGQPVLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAICVLLTLASSSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALASATLCGAAALFIAALICGMQIFKLHTNYS
                     "
     gene            3001..8242
                     /gene="iatD"
                     /locus_tag="N7B39_003168"
                     /pseudo
     CDS             3001..8242
                     /gene="iatD"
                     /locus_tag="N7B39_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168882.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter IatD"
     gene            complement(8399..8994)
                     /gene="kptA"
                     /locus_tag="N7B39_003169"
                     /pseudo
     CDS             complement(8399..8994)
                     /gene="kptA"
                     /locus_tag="N7B39_003169"
                     /inference="COORDINATES: protein motif:HMM:NF014001.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
     gene            9199..9882
                     /gene="yjiH"
                     /locus_tag="N7B39_003170"
     CDS             9199..9882
                     /gene="yjiH"
                     /locus_tag="N7B39_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HGU2423104.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            9879..10340
                     /gene="yjiG"
                     /locus_tag="N7B39_003171"
     CDS             9879..10340
                     /gene="yjiG"
                     /locus_tag="N7B39_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HGU2423105.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            10353..11525
                     /gene="iadA"
                     /locus_tag="N7B39_003172"
     CDS             10353..11525
                     /gene="iadA"
                     /locus_tag="N7B39_003172"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HGU2423106.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIVRAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            11647..12501
                     /gene="hypT"
                     /locus_tag="N7B39_003173"
                     /gene_synonym="qseD"
     CDS             11647..12501
                     /gene="hypT"
                     /locus_tag="N7B39_003173"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HGU2423107.1"
                     /translation="MTLEKCRNFSQAAVSRNVSQPAFSRRIRALEQAIGVELFNRQVT
                     PLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRKIKIAAAHSLSLGLLPSIIS
                     QMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLLEAPFDHIRLFESQLFPVCA
                     SDEHGEALFDLVQPHFPLLNYSRNSYMGRLINRTLTRHSELSFSTFFVSSMSELLKQV
                     ALDGCGIAWLPEYAIQQEIRSGQLVVLNRDELVIPIQAYAYRMNTRMNPVAERFWREL
                     RELEIVLS"
     gene            complement(12494..12886)
                     /gene="iraD"
                     /locus_tag="N7B39_003174"
     CDS             complement(12494..12886)
                     /gene="iraD"
                     /locus_tag="N7B39_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HGU2423108.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSTLPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(12883..12966)
                     /gene="idlP"
                     /locus_tag="N7B39_003175"
     CDS             complement(12883..12966)
                     /gene="idlP"
                     /locus_tag="N7B39_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HGU2423109.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            complement(13082..13225)
                     /gene="ytiD"
                     /locus_tag="N7B39_003176"
     CDS             complement(13082..13225)
                     /gene="ytiD"
                     /locus_tag="N7B39_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtiD"
                     /protein_id="HGU2423110.1"
                     /translation="MADYAEINNFPPELSSSGDKYFHLRNYSEYSEYTSGFFLSLMIF
                     IKS"
BASE COUNT         3484 a         3299 c         3200 g         3286 t
ORIGIN      
        1 tatcctattt gactacgcat ggtttgacaa tacaatattt ataaaaacgc tttttaaaaa
       61 tacctgtttt tacaatgtca gagcgaaaaa tgtctatctt gagggagcat atctgaacaa
      121 tgataatatc gtgaatcaag ccaataacag taccgagaaa caatccattg acagtaccga
      181 taaacaggcc aatgacagta cggtgcaaca atccattgac agtacggtgc aacaagccaa
      241 tgacagtacc gataaacaag ccaatgacaa tatcgataaa caggtcaatg acagtaccga
      301 taaacaagcc aagaacagta ccgagcaaca ggacagtaac agttttaatc aagcccgttt
      361 aaagaaagaa gtgaatagga gattttccat tccgggttta acgtcttatc agccaacata
      421 tatagttgaa gaatagttca ttaatcaccc aaacagagtg ggtatggcag acgatataga
      481 ttaacattta aacgttaacc gcctcatcta tcattcatag atgaggcgca tttatcagtc
      541 gatgtaagct gaaaatgttc tgccatccag agacaaccgc cgccccctgc ggcgtttact
      601 atttcatcgc ccgcgttaag aattcattaa gcttcatccg tatactcctt atctgtcgca
      661 ctgaatttga cgagaaaaaa gcaataataa ggagcaataa gttgaaaaag cgcatcacct
      721 ttatcgtttt tctttgcgcc atcgtggcgg caagcctgtt ttttgtgcaa tcctgcgtcc
      781 gtaaaagcca gcatgtcgca gggtttcaga attatcaggc cactatcgat ggtaaagaga
      841 ttacaggagt aactaaaaat atctcctcac tgacctggtc agcgcagagc aataccttat
      901 ttagcactat caacaaacct gcaaccatcg ttgaaatgac gaccgagggc gacttgatcc
      961 gcaccattcc gctcgatttt gttaaggatc tcgagacgat tgaatacatt ggtgataata
     1021 aatttgtcat cagtgatgaa cgtgactacg ccatttatgt gatttcactt aacgctgact
     1081 cggaagtgag catcctcaaa aaaattaaaa tcccattgca ggaaacacca actaactgtg
     1141 gctttgaagg gctggcttac tcctctcagg accatacctt ctggtttttt aaagagaaaa
     1201 atccgataga ggtctacaaa gtaaccgggc tattgcgtag cgatgagttg catatcagca
     1261 aagataaaac gttgcagcgt cagtttactt tagacgatgt ttctggcgcg gaatttaacc
     1321 cacagaaaaa tacgttgctg gtgttgtcac atgagtcacg cgccctgcag gaagtgaccg
     1381 tgagaggtga cgtgatcggc gaaatgtcgt taaccaaagg taagtatggc ctttcacaca
     1441 acatcaagca ggcggaaggg atagcgatgg atgatagcgg caacatctat atcgttggcg
     1501 aaccgaatct tttttatcgc tttacgtcga caaaatcacg ataacagtct ggcaggttct
     1561 ccttttttgt tattaacttg ttaactcaaa ataatacaaa ccaggcagaa catgaattcg
     1621 acatcgcatc ccgtagaacg tttttctttc agcaccgcgt tattcgggat gctggttctg
     1681 accttaggta tgggtttagg ccgctttctc tatacgccga tgctgccagt catgctggcg
     1741 gaaggcgagt tttcatttag cgaactctca tggatcgcca gtggtaacta tgccgggtat
     1801 ctggcgggga gcctgctgtt ttcattcggc gcgtttcatt taccctcacg cctgcgcccg
     1861 ttcctgttag cttccgccct cgcaaccgga ttattaatcc tcgcgatggc gtggctgccg
     1921 ccgtttcttc tggttttcat cattcgcttt ctggcgggag tcgccagcgc cgggatgttg
     1981 attttcggct caacactcat catgcaacat actcgccatc cctttgtcct tgcggcgcta
     2041 ttttctggtg ttggcgtcgg catcgctctg ggtaatgaat atgtgctggc aggcctgcat
     2101 tttgccctct cttcacaaac gttgtggcaa ggtgccggag cactttctgc cattatattg
     2161 cttgctctgg cgctgctcat cccgtcgaat aaacacgtta tcccgccagc gccattggca
     2221 aaaatcgcgc aacaacccat gagctggtgg ttactggcga ttctgtatgg tctggcgggt
     2281 tttggttata tcatcgtcgc cacctacctg ccgctcatgg cgaaagacgc gggccagcct
     2341 gtgttgacgg ctcacctctg gacactggtt ggcttgtcga ttgtcccagg ttgctttggc
     2401 tggctgtggg cagccaaacg gtggggagca ttaccttgcc tgaccgcgaa tttgctggtg
     2461 caggcgatct gcgtgctgtt aaccctcgcc agcagctctc ctttattact catcatcagc
     2521 agtattggtt ttggcggcac ctttatggga acgacctcgc tggtgatgac catcgcccgc
     2581 cagcttagcg tgccgggaaa tcttaacctt ttgggctttg tgacactcat ttatggtatc
     2641 gggcaaattc ttggcccggc gctgaccagt atgctcggca acggaacgtc ggcgctcgcc
     2701 agcgccacac tctgcggcgc agcggcgcta tttatcgcag cattaatctg cgggatgcaa
     2761 atattcaaat tgcatacgaa ttattcttaa gtaatttaac taatttaact tttcagcaca
     2821 tcacgtaaaa taattaaaat atcctttttc tttggaggta ctcagccacc gccggtatta
     2881 ttcctggttt agcaattcca cggctaattc attatttctg gtcatattat agtcaggatc
     2941 gcattatatc tccgtgctag aaaaaacaag tttaattctg ttttttcacg gaaggtcact
     3001 atggcttcga caaatgcgca tcaaataaaa aataatgatc aaaactcttt atgtggtctg
     3061 ggtgacaaaa tacgccgcct gaccgccgga gtttgtttat ttacacaatt ttttttcccc
     3121 gtcatggcga cggcacaaaa tgtggtacat gctaaacctc agacgacagt ttcatctgcc
     3181 cccccctcta ctagaaaata atactgtgcc ttacacgctt ggtgcgctgg aatcagcgca
     3241 aagcgttgct aatcgcttcg gtatttcact ggaggagctt cgtcgtctta atcagttccg
     3301 tacttttgct cgcggctttg ataacgtgcg ccagggtgaa gaactggatg ttccggcaac
     3361 aacctcgcag aaaagccatg agcaacaaaa tgccgtaccg cctgcgaatg gcgaaaacac
     3421 gctggagaat caaatagcca gcacctcgca gcgagttggc actctgcttt cacaagatat
     3481 gaatagtgag caggccagcg gcatggcgcg tggttgggcg tcttcagaag cctcaggcgc
     3541 gatgactgat tggttaaata actttggtac tgcgagaatc tctctgggtg tggatgaaga
     3601 ttttagcctg aaaaattcgc aattcgactt cctgcatccg tggtatgaca cacctgatta
     3661 tctgctcttc agccagcata cccttcaccg aacagacgat cgtacccaga tcaacaccgg
     3721 tttgggctgg cgtcatttca cctccagctg gatgtcaggc atcaaccttt tttttgacca
     3781 cgacctgagc cgctatcact cccgcgcagg gcttggcgca gaatactggc gtgattatct
     3841 gaagttgagc agcaacgctt atatcggcct gaccggctgg cgtagcgcac cagaattgga
     3901 taacgacttc gaagcccgcc cggccaacgg ctgggattta cgcgcggaag gctggttacc
     3961 tgcctggcca caactggggg gaaaactggt ctatgaacaa tactatggcg atgaagtggc
     4021 gctgtttgac aagaatgatc gtcaaagtaa cccccatgct attaccgcag gcctcaacta
     4081 tacccccttc ccgcttctga ctctcagtgc ggaacagcgt caggggaagc aaggtgaaaa
     4141 tgacacacgt tttgccgttg atctgacctg gcaacccagc agttcaatgc agaaacagct
     4201 taatccggac gaagtggccg gacggcgcag tctggccggt agtcgttatg acctgattga
     4261 tcgcaacaac aacatcgttc tggaataccg caagaaagag ctgattcgcc tgagtctgct
     4321 ggatccggtg aaagggaagt ctggagaaat aaaaccgctg gtttcctcgc tacagaccaa
     4381 atatgccctt aaaggctata acatcgaagc cgctgcgctg gaagctgccg gaggtaaagt
     4441 cagcacgtct ggaaaagata tcacggtcac gctgccaggt taccgcttca ctaacacccc
     4501 agaaaccgat aatacatggt cgatagacgt taccgccgag gatgtaaaag gtaacctgtc
     4561 acggcatgaa caaagcatgg tagttattca ggctccgaca ttaagccaga aagattctct
     4621 gttatccgtc aatccgctaa ccgtggctgc agataaaaaa tcgacgacca cattgaccgt
     4681 tactgcgcac gattccgacg gaactccggt gccggggctg gcgctgcaaa cccgcagtga
     4741 aggcgttcag gatatcaccc tgtctgattg gacagataac ggtgatggta gttacacaca
     4801 gatactgacc gccggaacga catcaggttc agtaacactg acgccgcaaa ttaacggtga
     4861 gagtgcggta aaagaatcca tcgtcgttaa tatcgtccct gttgtctcat cccgcgacca
     4921 ttcatcaata acaattgata acgtatcgta ttatgccgga gacgacatca aggttagggt
     4981 ggaactgaaa gacgatagca atcaaccggt tgcatatcaa aaagaggaat tggtaaaagc
     5041 cgttactgtc gaaaacagca aacctggcgc cacgattgtc tggcacgaag agcagccggg
     5101 ggtttatgcc gcgaattatc cggcctataa gcaagggact gcactaaggg cacaacttag
     5161 ccttcacaac tggaatgctc cactgcaatc gcatatttat aacattgagg caaaccagaa
     5221 taaggctcgc gttgccacat tatcagcgac aaataatgac gtttacgccg ataaaaagac
     5281 atttaatacc ctcacgatca acgtcactga tgagagtgat aatcccctga caaatcatca
     5341 ggtcaccttt aagaatgaaa aaggaagcgc ggagtttgtc gaaccgccgc agcaaaatac
     5401 ggatgcatat ggtgttgcca caataaacat ggtaagtcag gttgcggaag aaaatacgat
     5461 tagcgccacg ctgccaaatg gtttttcaca acggataatt gcgaaattcg ttagcgattc
     5521 gagtacgcca aaattcaaac aactggttgc cgatccagat accattattg ctggcaacag
     5581 ccagggcagt actctgaccg ccatcatcac agactttcat aacaacccgt taaaagatat
     5641 gaaagtgaat tttgtggcac ctggtggctc gcaactggac aacacgaccg ccacaacaga
     5701 ccagtccggt attgtgcggg tgcacctgac cagttcaaaa gctggtagct attccgtcga
     5761 tgcctcgctt gaggtggata aaaatattca ccagtcggtc acgatcaccg tggtcccaaa
     5821 cagggaacaa tcggtaatga ccttgaatgc cgggtcgggc agtgcgatcg ctaacaatac
     5881 aaatatcgtt accctgactg ccagtgtgaa agatgtttat ggacacccgt tgccggatga
     5941 ggatgtgaaa tttaccttgc cagcctccat gaccgggaac ttcacgctaa gtagtgaaac
     6001 cgcccgcacc gatgcaaacg gtgatgccgt ggtcacattg cgaggcacaa aagcgggtga
     6061 gtttacagtt acggcgacgc tgaccagaaa taataccgtt gcttatcagc aagtcacttt
     6121 tattggggat acaaacagtg cgcagctcca gccgctgact gcctcattaa attccattgt
     6181 tgcgggtaac agtacgggga gtaccctgac ggcaacgatc ctggacgctt accaaaatcc
     6241 gcttaaagac cagttggtca ctttccagag taacgatgtc actctaagcg aaacagaagt
     6301 caccaccaat acgctgggtc aggcgacggt aacaatgacc agcaatattg ccggacaaca
     6361 taacgtcgtg gtgagccgga aagcgcaagc ttccgataat aaaacgttta gtttatcagt
     6421 gctaccggat gaaagttcgg cgaaggtaat aagtataacc ggagccgaaa aaacgataac
     6481 ggtgggcgaa aacatcacgc tacggatact cgtccaggac gcgtttaaca atgtaatcgc
     6541 gggtcaacgc gtcagattaa gtgcgcagcc aacaactaac attacgatag gcgatacggc
     6601 ttacaccgat aataacggtt atgcgtacgt taaccttctc agcacccaac ctggggttta
     6661 tcaggtgacg gcaacgctgg acaataacag tagtagtaag gttgacgtga atgtggcaaa
     6721 tggcaaactc gagttaacat catcgaaacc agaaactacg gtccataata gtgagggtat
     6781 tacgctgacc gcaacggcga gaaatgcgcg gggtgaattg atgccagggc aaattatcac
     6841 ctttagcgta acgcctgaag gtgcaacgct aagcaataca ggggaagtcc ttactgacca
     6901 gtcaggtcag gccaaagtga cgctgaccag tgacaaagtg aatgtctata ccgttacggc
     6961 cataatgggc aaagatgttc ccgttcagag ccaggtaacg gttgcggtta aggcagatgc
     7021 taaaacggca catgttgtga gcgtcgtggc ttctcctgac accatcaccg ccgacggcat
     7081 cgatagcagc accatcactt cacgagtaga agatgattac ggattcccgg ttgaaggtgt
     7141 cgatattagt catggcttag acaccaaagg cagcccggta gttaatattc caactacgcg
     7201 taccgatcag tccgggcaag tcacggcgac aataaccagt acattggcag aaaccttaac
     7261 agtcaatgtg caagttcctg gcacagccaa ccaatccgca accattacat tggttgccgg
     7321 cacggccgat gaaagtaagt caattttgaa atccgatgtt gacactctga aggctgacta
     7381 ccagcagagc gcaaaactta cgctaacatt gcaagacaag tacggtaacc cgatagtgac
     7441 gtctgatcat ctggaatttg tccagtcagg ccccttcgtg aactttctca agttgagcga
     7501 tattgattac agccaaagaa attatggcga gtacaccgtg actgtcactg gcggaaaaga
     7561 gggaacagcg acactcattc ccatgctgaa cggggttcat caggcaaact taagcatatc
     7621 gctgaatctc atccaatcga taaaagaaat gtccggtcat gtcactgcaa acaaccatac
     7681 cttctccacg gctaaattcc cgagcgaagg ctttgcagga gcgtattaca cactcaacaa
     7741 tgataacttt gaagcgggta aaaccgttga tgattatatg ttttcaagtt cacagggttg
     7801 ggtgtctgtc gatgcttcgg gtaaagtttc tttcgcaaat atcggcgatc aaacgtcagt
     7861 cacaataagc gctgttcccc gacaaggagg tacaacctac cagaccttaa ttaagctgaa
     7921 aggctggtgg gtgaataatg gaaatcatac caatatctgg ctagctgcca atgcgctctg
     7981 tcatgctaaa aatgatggat ataatcttcc tggcatcaca catttgacgt ctggcgaaaa
     8041 caaacgcacg cagggatcac tgtatggtga atgggggaac gttggagcgt tttccagtaa
     8101 ttcgcaattt acaccgggag cttactggac aagtgaatct gatgattaca gtcggcacta
     8161 ctatgtgcag atgctaaccg gtatgaccgg aagcgacgct gattccagcc cccaactgac
     8221 cgcctgccgt aaatcacttt aatttcagca gttgttatta aaggataccc ttttacgagg
     8281 gtatcctttc agactgataa caaacgctac attgccggat gtgctgcgct tatcaggcct
     8341 atataacccc tgcaatatat tgaatttatt gtttttgtac cccagccggc ataaacgact
     8401 actcaagaaa ctcaacagca accgttgatg tcagccagac cccgttttcc gcttgccaga
     8461 aagggatgcc tcgtttcgcc atttcctgtg ctttgacggt taagacaacc ggcgatccgt
     8521 gtcttgctcc cactttgcgt gccgttgctt catcagcaga gagatgcacg taatgacgat
     8581 cccctgcaat cagcccctgt ttttttatct cgtcaaggaa acggcttgcc gtaccgtggt
     8641 agagaaactg tggcggtgtt ttttcggcaa aggaaatagc gacctgcgaa gtcgaatgcc
     8701 cctgaaccgc gcggatgcac ctaccatcac tggaataact aaaacgcttt tatcgctggt
     8761 tgcgaccacg gtatccagca atgcacgggt taatcgcttg cccgcttttt gggcacaaag
     8821 tatgagctta tcgatattcg cccatccttc acggtccagt acgataccaa tcgcctccgg
     8881 tttgtgtcgg aggacaaaac ttaaaaactt gctcgtatcc gccagctctt tttcgttgta
     8941 ctttgccatc gcgttttcgt ccctggattt tctgttttta tttaccaatg tcattgtcac
     9001 tgggtttatg aattttctgc aactcaatcc tgcgcagaac cttttccaca cagcgtaatt
     9061 tttcccctat gcaaaaaacg catagccaga aacatacttg cattttcccc atacccacat
     9121 ggctgataag gtttttaagt tccaggaatt ttcctggctc aagcaacaca caaacacaaa
     9181 aaaacaatgg ggattgttat gacacaacaa ggggatgctg tagccgggga gctggcgaca
     9241 gaaaaagtgg gcattaaagg gtatctggct ttttttctca ccataatctt tttttccggt
     9301 gttttttccg gaacagacag ctggtggcgc gtttttgatt tttccgttct gaacggatcg
     9361 tttgggcaac tgcctggtgc gaatggagcc accacctcat ttcgcggcgc gggtggcgcg
     9421 ggggctaaag atggctttct ctttgcgctg gagctggcac cgtcagtcat tctctctctg
     9481 gggattattt caattaccga tggtcttggt ggattacgcg ccgcgcagca gttaatgaca
     9541 cctgtgctaa aaccgttgtt gggcattcca gggatctgct cgctggcgct gatcgctaac
     9601 ttgcaaaaca ctgatgccgc cgccggtatg accaaagagc tggcgcagga aggtgaaatt
     9661 accgaacgcg ataaagtgat tttcgccgca tatcaaacca gcggtagcgc catcatcacc
     9721 aactacttct cttccggcgt cgcggtgttc gcctttctgg gcacgtccgt aatcgtacct
     9781 ttagccgtca tcctggtgtt taaatttgtc ggagccaaca ttttgcgcgt ctggcttaac
     9841 ttcgaagaac gtcgcaaccc aacgcaagga gcgcaagcat gacaactcag gtccgcaaaa
     9901 acgtcatgga tatgtttatc gatggcgcgc gccgtggctt tactatcgcc acgaccaacc
     9961 tgctgccaaa cgtggtgatg gcgtttgtca tcattcaggc gctgaaaatt accggcctgc
    10021 tcgactgggt ggggcatatc tgtgaacccg taatggcgct gtggggatta cccggtgaag
    10081 cggcaaccgt ccttttagcc gcattaatga gtatgggcgg cgcagtcggt gttgccgcca
    10141 gtctggcaac cgctggcgca ttaaccggtc acgatgtcac cgttttactg cccgcgatgt
    10201 atttaatggg caacccggtg caaaatgtcg gtcgctgcct ggggacagcc gaagtgaatg
    10261 ccaaatatta cccgcatatc attacggtct gcgtgattaa cgcattgctg tcgatctggg
    10321 ttatgcagct tattgtttaa caaggagtta ccatgattga ttataccgca gccggtttta
    10381 ccctgctgca gggagcgcat ttgtatgcgc cggaagatcg gggaatttgc gatgtcctcg
    10441 tcgctaacgg caaaattatc gccgttgcca gtaatatccc ttctgacatt gtaccggact
    10501 gcacggttgt cgatctcagc gggcagatcc tctgcccagg ttttattgat caacacgtcc
    10561 atttgattgg cggtggcggc gaagcaggtc ccacgacgcg cacgccggaa gtggcgctaa
    10621 gtcgcctgac ggaagcgggc gtcacgtcag tggttggtct gctgggcacc gactctatct
    10681 ctcgccaccc ggaatccctg ctcgccaaga cccgtgcgct caatgaagaa ggcatcagcg
    10741 cctggatgct gaccggcgct tatcatgtcc cttcccgcac cattacgggt tccgtggaaa
    10801 aagacgtggc gattatcgat cgtgtgattg gcgtgaaatg tgccatctct gatcaccgtt
    10861 ctgccgcacc ggacgtttat cacctggcta atatggcggc agaatcccgc gttggcggtt
    10921 tgctcggcgg taaacctggc gtcaccgtgt tccacatggg cgacagtaaa aaggcattac
    10981 agcctattta tgacctgctg gaaaactgcg atgtgccgat cagcaagctg ctgccgaccc
    11041 acgttaaccg caacgtaccg ttgtttgagc aggcgctgga gttcgcgcgc aaaggcggca
    11101 ccatcgatat caccagcagc attgacgaac cggtcgcccc tgccgaaggt attgtccgcg
    11161 ccgttcaggc gggtattccg ctggcacgcg tcaccctcag ctccgacggc aacggtagcc
    11221 agccgttctt cgatgatgaa gggaatttaa cccatatcgg cgttgcaggt tttgaaacgt
    11281 tgctggaaac cgtgcaggtg ctggttaaag actatgattt cagtatcagc gatgccctgc
    11341 gcccgctcac cagtagcgta gccggtttcc ttaacctgac cgggaaaggc gaaattctgc
    11401 caggcaatga cgcagactta ctggtcatga cgccagaatt gcgcattgag caggtatacg
    11461 ctcgcggcaa actgatggtc aaagacggca aagcctgcgt gaaaggaacg tttgaaacgg
    11521 cttaaacggt tgtcagccgc cctaatggac gtagaatgcc cccaagggcg gctgacagag
    11581 taaaacgtaa tggatgactg tggtgcggtt ttgcataata ttgaaaccaa atggctttaa
    11641 gattttctga ccctggaaaa atgccgcaat ttttcccagg cggcagtcag tcgtaacgtc
    11701 tcgcaaccgg cattcagccg ccgcatccgc gcgctggaac aggcgattgg cgttgaattg
    11761 tttaaccgcc aggtgacgcc gctgcaactc tcagaacaag ggaaaatctt ccattcgcag
    11821 atccgccatc tgttgcaaca gttagaaagc aaccttgcag agctgcgtgg cggcagcgat
    11881 tacgcgcaac gtaaaatcaa gatagccgct gcacactctc tttccctcgg tctgttaccg
    11941 tccattatca gccagatgcc gccgctcttt acctgggcga ttgaagccat tgatgtcgat
    12001 gaagcggtcg ataaactgcg tgaaggacaa agtgactgta ttttttcctt tcacgacgaa
    12061 gatttgctgg aagcgccgtt tgaccacatt cgcttatttg aatctcaatt gttccctgtc
    12121 tgcgccagtg acgaacatgg agaagcactt tttgacctcg tgcagccaca ctttccgtta
    12181 ctgaattaca gccgcaactc ctacatgggg cgattgatta atcgcaccct gacgcgccac
    12241 agtgagttaa gtttcagcac cttttttgtc tcttcgatga gcgagctttt aaagcaggtt
    12301 gccctcgacg gctgtgggat tgcctggctg ccggagtatg ccatccaaca agaaattcgc
    12361 agcgggcagc tcgttgtgct gaatcgggac gaactggtga tcccgattca ggcctacgca
    12421 taccggatga atacccgcat gaatccagtt gccgaacgct tctggcgtga actgcgcgag
    12481 ctggagattg tgcttagctg acattctcaa gcgtcgcact gcgccaaccg ccattatccc
    12541 agaccagatc aaacgttagc ggttcatcgt cgtggtacag cattatctca aggctgatcg
    12601 ccagagtgtg gcagtacgct tctttcaaat taacctgcaa cgccgcaatc cgtggctcat
    12661 gaaataacag tgcagaacgc agctgacggc aataccagct ctggagatgt ttgtcgctcg
    12721 ggttatgcac ttgccactcg ggcatgcctg aaacaagtaa tgacggtaat gtcgaatgag
    12781 gagaattaaa aagagtgagg agatcgctgc ataccgattt acgcagcaat gacgttttat
    12841 tgcctgaaat ttcaggtaaa acagcctgaa gtgattggcg catcattttg cgcactcctg
    12901 acgtttagca acatatgcgg cttgccctga acaccgggca ccactgtatt gatgctcatt
    12961 ttccatagcc attccctttt gttcattaca tccatgtcag tctgagacag gcctgaatcc
    13021 tgtcagcaaa gtataaaacc ttacttgcct atgaatatct actgactttt caggaaaaaa
    13081 gtcatgattt gataaaaatc atcaaactca aaaaaaaacc gctagtatat tctgaatatt
    13141 ccgaatagtt acgtagatga aaatacttgt caccgctgct acttaattcc ggtgggaaat
    13201 tatttatttc cgcataatca gccatgtatt atttgaaaca attagtttgt aaataatata
    13261 aattgataa
//
LOCUS       DBFIFI010000118        13266 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.118, whole genome shotgun sequence.
ACCESSION   DBFIFI010000118 DBFIFI010000000
VERSION     DBFIFI010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13266
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.118"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            267..1385
                     /locus_tag="N7B39_003177"
     CDS             267..1385
                     /locus_tag="N7B39_003177"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HGU2423111.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHKEKD
                     YLLYKFNRFQACRYGLEGVITDPYTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1451..1699
                     /locus_tag="N7B39_003178"
     CDS             1451..1699
                     /locus_tag="N7B39_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HGU2423112.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTTFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            1764..2132
                     /locus_tag="N7B39_003179"
     CDS             1764..2132
                     /locus_tag="N7B39_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HGU2423113.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2226..2879
                     /gene="nfsB"
                     /locus_tag="N7B39_003180"
     CDS             2226..2879
                     /gene="nfsB"
                     /locus_tag="N7B39_003180"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HGU2423114.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            2987..4234
                     /gene="ybdG"
                     /locus_tag="N7B39_003181"
     CDS             2987..4234
                     /gene="ybdG"
                     /locus_tag="N7B39_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HGU2423115.1"
                     /translation="MQDLISHVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4302..5678)
                     /gene="pheP"
                     /locus_tag="N7B39_003182"
     CDS             complement(4302..5678)
                     /gene="pheP"
                     /locus_tag="N7B39_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HGU2423116.1"
                     /translation="MKNASNVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLAMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(5780..8917)
                     /gene="cusA"
                     /locus_tag="N7B39_003183"
     CDS             complement(5780..8917)
                     /gene="cusA"
                     /locus_tag="N7B39_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HGU2423117.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKIQVGPEMRRGIA
                     ELNGEVVGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRAID
                     NLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMSLG
                     GIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITNASVEVGPALFI
                     SLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGKIP
                     PESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQINEG
                     DLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMVET
                     TIQLKPQEQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIGIK
                     VSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTVAD
                     VQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITLAD
                     VADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSVAF
                     SGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWLLW
                     WMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEALH
                     HGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFIIP
                     AAYKLMWLHRHRVRK"
     gene            complement(8929..10152)
                     /gene="cusB"
                     /locus_tag="N7B39_003184"
     CDS             complement(8929..10152)
                     /gene="cusB"
                     /locus_tag="N7B39_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HGU2423118.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGAPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(10168..10500)
                     /gene="cusF"
                     /locus_tag="N7B39_003185"
     CDS             complement(10168..10500)
                     /gene="cusF"
                     /locus_tag="N7B39_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HGU2423119.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGFDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSGIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(10524..11906)
                     /gene="cusC"
                     /locus_tag="N7B39_003186"
     CDS             complement(10524..11906)
                     /gene="cusC"
                     /locus_tag="N7B39_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HGU2423120.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALENNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTVLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            12063..12746
                     /gene="cusR"
                     /locus_tag="N7B39_003187"
     CDS             12063..12746
                     /gene="cusR"
                     /locus_tag="N7B39_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HGU2423121.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            12736..>13266
                     /gene="cusS"
                     /locus_tag="N7B39_003188"
     CDS             12736..>13266
                     /gene="cusS"
                     /locus_tag="N7B39_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HGU2423122.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVFLLSGPTMMMPGHGHGHMEHSNWRMISLPVGPLVD
                     GKPIYTLYIALSIDFHL"
BASE COUNT         3157 a         3621 c         3283 g         3205 t
ORIGIN      
        1 aatagctgtt tatttataca gtaaatcctg tttcggactt ctcactcctc atttaatcaa
       61 ctgaagctga gcccgttttt tctgcatgcc tcaatcgtca aaattcttcg ccaccagcca
      121 gccgtgccat aacttgtcac attgcaaaag tagccaaatt gcttcagtca ccatcaccaa
      181 aaaaagaaac attccagaac ttccgcatct cgcccagccg ctccgacttc tatactgaat
      241 agaaaactcc aacataagag aaacctatgc cattacccga ttttcatgtt tctgaacctt
      301 ttaccctcgg tattgaactg gaaatgcagg tggttaatcc gccgggctat gacttaagcc
      361 aggactcttc aatgctgatt gacgcggtta aaaataagat cacggccgga gaggtaaagc
      421 acgatatcac cgaaagtatg ctggagctgg cgacggatgt ttgccgtgat atcaaccagg
      481 ctgccgggca attttcagcg atgcagaaag tcgtattgca ggcagccgca gaccatcatc
      541 tggaaatttg cggcggtggc acgcacccgt ttcagaaatg gcagcgtcag gaggtatgcg
      601 acaacgaacg ctatcaacga acgctggaaa actttggcta tctcatccag caggcgaccg
      661 tttttggtca gcatgtccat gttggctgtg ccagtggcga tgacgccatt tatttgctgc
      721 acggcttgtc acggtttgtg ccgcacttta tcgccctttc cgccgcgtcg ccatatatgc
      781 agggaacgga tacgcgtttt gcctcctcac gaccgaatat tttttccgcc tttcctgata
      841 atggcccgat gccgtgggtc agtaactggc aacaatttga agccctgttt cgctgtctga
      901 gttacaccac gatgatcgac agcattaaag atctgcactg ggatattcgc cccagtcctc
      961 attttggcac ggtggaggtt cgggtgatgg ataccccgtt aacccttagc cacgcggtaa
     1021 atatggcggg attaattcag gccaccgccc actggttact gacagaacgc ccgttcaaac
     1081 ataaggagaa agattacctg ctgtataaat tcaaccgttt ccaggcctgc cgctatgggc
     1141 tggaaggcgt cattaccgat ccgtacactg gcgatcgtcg accactaacg gaagacacct
     1201 tgcgattgct ggaaaaaatc gccccttctg cacataaaat tggtgcatcg agcgcgattg
     1261 aggccctgca tcgccaggtc gtcagcggtc tgaatgaagc gcagctgatg cgcgatttcg
     1321 tcgccgatgg cggctcgctg attgggctgg tgaaaaagca ttgtgagatc tgggccggtg
     1381 actaagccgg gaattgcctt acagaagaat atccctgaca atggtgtttt aacttacatt
     1441 taacaatgtt atgaaacacc ctttagaaac cttgaccacc gcagcaggca ttttgctgat
     1501 ggctttcctc tcttgcctgc tgctgcccgc ccccgcactg gggcttacgc tggcacaaaa
     1561 actggtgacc acgttccatc tgatggatct tagtcagctt tacactttat tgttttgtct
     1621 gtggttttta gtgctgggcg ctattgagta ttttgttctg cgctttatct ggcgacgctg
     1681 gttctcgctg gcggattaaa cgtcgattat cgccgtactt cgcggcatac tttgcttatt
     1741 ctccttcgcc taaaggaacg tttatggata agcaatcact gcacgaaacg gcgaaacgcc
     1801 tggcccttga gttacccttt gtcgagcttt gctggccttt tggcccggag ttcgatgttt
     1861 ttaaaattgg cggcaagatt tttatgctgt cgtcggagct acgcggcgtc ccctttatca
     1921 atctgaagtc cgatccacaa aaatccctgt taaatcagca aatataccca agcattaagc
     1981 cagggtatca catgaataaa aagcactgga tttcggtgta tcccggcgag gaaatctccg
     2041 aagcgttact tcgcgatctg atcaacgatt cgtggaatct ggtggttgat ggtctggcta
     2101 aacgcgatca aaaaagagtg cgtccaggct aaagcggaaa tctatagcgc atttttctcg
     2161 cttaccattt ctcgttgaac cttgtaatct gctggcacgc aaaattactt tcacatggag
     2221 tctttatgga tatcatttct gtcgccttaa agcgtcattc cactaaggca tttgatgcca
     2281 gcaaaaaact taccccggaa caggccgagc agatcaaaac tctcctgcaa tacagcccat
     2341 ccagcaccaa ctcccagccg tggcatttta ttgttgccag cacggaagaa ggtaaagcgc
     2401 gtgttgccaa atccgctgcc ggtaattatg tgttcaacga acgtaaaata cttgatgcct
     2461 cgcacgtcgt ggtgttctgt gcaaaaaccg cgatggacga tgcctggctg aagctggttg
     2521 ttgaccagga agatgctgat ggccgctttg ccacgccgga agcgaaagcc gcgaacgata
     2581 aaggtcgcaa gttcttcgcc gatatgcacc gtaaagatct gcatgatgat gcagagtgga
     2641 tggcaaaaca ggtttatctc aacgtcggta atttcctgct cggcgtggcg gctctgggtc
     2701 tggacgcggt acccatcgaa ggttttgacg ccgccatcct cgatgcagaa tttggtctga
     2761 aagagaaagg ctacaccagt ctggtggtag ttccggtggg tcatcacagc gttgaagatt
     2821 ttaacgctac gctgccgaaa tctcgtctgc cgcaaaacat taccttaacc gaagtgtaat
     2881 tctcccttgc cgggcatctg cccggctatt tcccctcaga ttctcctgat ttgcataacc
     2941 ctgtttcagc cgtcatcata ggctgctgtt gtataaagga gacgttatgc aggatttaat
     3001 atcccatgtt gaagatttag cgggtattga gatcgatcac accacctcga tggtgatgat
     3061 tttcggtatt atttttctga ccgccgtcgt ggtgcatatt attttgcatt gggtggtact
     3121 gcggaccttc gaaaaacgtg ccatcgccag ttcacggctt tggttgcaaa tcattaccca
     3181 gaataaactc ttccaccgtt tagcttttac cctgcagggg attatcgtca atattcaggc
     3241 ggtattctgg ctgcaaaaag gcaccgaagc ggcagatatt ctgactacct gcgcgcagtt
     3301 gtggatcatg atgtatgcgc tgctttcagt cttctcgctg ctggatgtta ttttgaatct
     3361 ggcgcagaaa ttcccggcag catctcagtt accgctgaaa gggatatttc aggggattaa
     3421 actgatcggc gcgattctgg tcggcatttt gatgatctcg ctgctgattg gtcagtcgcc
     3481 agcgattctg atcagcggtc ttggtgcaat ggctgccgtg ctgatgttgg tatttaaaga
     3541 tccgattctt ggtctggtgg caggtattca gctttccgcg aacgatatgc tgaaactggg
     3601 cgactggctg gagatgccga aatacggcgc ggatggcgcg gtgatcgata ttgggttaac
     3661 caccgtcaag gtgcgtaact gggacaatac cattaccact attcccacct ggtctctggt
     3721 ttctgactcc tttaaaaact ggagcgggat gtcagcatct ggcgggcgac gtattaagcg
     3781 cagtatcagt attgatgtca ccagtattcg ttttcttgat gaagacgaaa tgcaacgtct
     3841 gaataaagcg catttgttaa agccttattt aaccagccgc catcaggaaa ttaatgagtg
     3901 gaatcgccag caaggttcta cggagtcggt attaaacctg cgccgaatga ccaatattgg
     3961 aacctttcgt gcctatctga acgaatatct gcgtaaccat ccgcggattc gtaaagatat
     4021 gaccttaatg gtacgccaac tggctccagg tgataacggt ttaccgctcg agatctatgc
     4081 gtttaccaac accgtggtgt ggctggaata tgaaagcatt caggctgata tattcgatca
     4141 catatttgcg attgtcgaag agtttggtct gcgacttcat cagtcgccaa ccggcaatga
     4201 tattcgctct ctggcgggtg catttaagca gtaattaaaa aaaccgctct catcgaatgg
     4261 atgagagcgg acatcgtttt gtcggatgta gcgtgaatgc cttatttccg acgcagcgtt
     4321 ttaaatgcta caaacaggaa tacaatccac accggcagca ggatcgctga caagcgcata
     4381 tcatccatcg tgcacatcag cagcaaaatc atggcgagga aggcgatgca aagatagttg
     4441 ccgaacggat agagcagcgc cttaaactgt gtttcacgcc cctgacgtcg catcgctgca
     4501 cgaaaacgca gatgcgccag acagatcata atccagttca acagcagcgt tgctaccacc
     4561 agcgccatca gcagaccaaa cgctttttgc ggcagcagat agttgattaa caccaccagc
     4621 gaagtgatcg ctccggaaag catcagcgag ttaatcggca cgccgcgacg gctaacacgg
     4681 gtaaggaact tcggcgcatt accctgcaca gaaaggccaa acagcatgcg gctgttagag
     4741 taaaccccgc tgttatacac tgacagcgat gctaccagaa tgacgaagtt cagcgcagaa
     4801 gctaccacgt tgctgtcgag attatggaaa atcatcacaa acgggctact gttggatttc
     4861 acttccaccc acggatagag cgccagtaaa accaccagtg aaccgatgta aaacagcagg
     4921 atgcgataca ccacctgatt taccgctttt ggaatgcttt tttccggatc gcgcgcttca
     4981 gcggcagtaa tcccaatcag ctccagaccg ccgaaggaga acataattac cgccagcgac
     5041 aaaatcagcc cattccagcc ggtggcgaag aaaccaccgt agcgccagag gttgtcgata
     5101 ctggctttct cgccgccgtg accagaaaac agcagccaca ggccaaagcc gatcataccg
     5161 atgattgcca gcactttaat cagcgcaaac cagaactcgg tttcgccata taagcgcacg
     5221 ttcaccaggt taacggcgtt gatgataata aagaaggcgg cagcccaaat ccacgttgga
     5281 acatccggga accagtactg catatagatg cccgcagcgg tcagctctgc cattcccacc
     5341 agcacgaaca tcacccagta gttccagccg gagaggaacc cggcgaacgg tccccagtat
     5401 ttataggcaa agtgggcaaa tgaaccggat accggctctt cgaccaccat ctcgccgagc
     5461 tggcgcataa tcaggaaagc gatgatcccg gcgacgccgt agcccagcaa tacagccgga
     5521 cccgccatct gaatcgccgg gccaatgcca agaaacagac cagtaccaat tgcgccaccc
     5581 agcgcaatca gttgaatatg acggttatgt aatccgcgat gaagcgtcgg ctcttgattc
     5641 gacgcagtat cttccgatac gtttgacgcg tttttcacgc ctttcccctg tgtgtctttt
     5701 ttgttgaggg gcacctttta acatttagtg cccatcgtcg caagacacaa tccacacggt
     5761 taaaccgggt atcctgcttt tatttccgta cccgatgtcg gtgcagccac atcagcttat
     5821 acgctgccgg gataataaac agcgacagca aaggcgcggt gatcatgccg ccaatcatcg
     5881 gcgcggcaat ccggctcatc acctctgatc ctgcccccgt tccccacaga atcggcagca
     5941 gaccagcgat aatcaccgcc accgtcatcg ctttcgggcg cacgcgcagg accgcgccgt
     6001 gatgtaacgc ctcatccagc ttctgctcgc tgaatgtttg cggattattc aacgacggct
     6061 cggcctctat ggcgtgacgt aaatacatca gcatcaccac gccaaattcg gcggcgaccc
     6121 cggcaagggc aataaaaccg gtgcccgtcg ccacagaaag atgaaagccc atccaccaca
     6181 gcaaccagat gccgcccacc agcgcaaacg gtacgctgct gataatcagc aacgcttcgc
     6241 ccacccgacg gaacgccaga tacaacagca cgaagatgat catcaacgtc atcggcacca
     6301 tgagtttcag cttatggttg gcgcgctcca gtagctcgaa ctgcccggag aatgccacgc
     6361 tggtgcccgg ttttaactgc actttttcag ctatcgcttt ttgcaaatcg tgaaccaccg
     6421 acaccatgtc acgatcgcgg gcatcgatat aaatccagct cgtcgggcgc gcattctcgg
     6481 ttttcagcat cgacggtccg gtagagactt taacgtcggc cacgtctgcc agggtgattt
     6541 gctgcttcat cggcgtcagg atcggcaact ggcgcagtgc ctgcggacta tcgcgccagc
     6601 tttgcggata acgcagatta attggataac gggcaatccc ttccaccgtt tcgccaacca
     6661 tcgccccgcc caccgcagaa gtcacaaaca actgcacatc cgccaccgtc ataccgtaac
     6721 gtgcggcttt ttcacggtta atctcaacgt tgatatagcg cccaccttcc agccgctcgg
     6781 caagcgcaga agccacgcct ggcaccgttc gtgccacctc ttcaatttgc tccgccatcg
     6841 cgtcgatatc cgccagcaca gtgccggaaa cttttatgcc gatagggctt ttaatgccgg
     6901 ttgagagcat atcgatacgg ttacgaattg gcggcaccca cagattcgcc agccccggca
     6961 gacgcacggt gttatccagt tcctcaatga ttttgtccat tgtcatgcct ggccgccact
     7021 gctcctgcgg cttaagctgg atggtcgttt ctaccatctc cagcggagca gaatcggtgg
     7081 cggtttccgc tttcccggtt ttgccaaata cccgcgccac ttcaggtacg ctcataatta
     7141 gcttgtcggt tttttgcagc atactcgccg cctctgctgc ggaaatcccc ggcagcgtcg
     7201 atggcatata caacaagtcg ccttcattga tctgcggtaa aaattccccg ccaactttat
     7261 tgagcggcca gagaaccgtc agcaccgaaa gcgctgccac caacagcgtg gttttcggcc
     7321 agtgcagtac tttcagcaac agcggatgat aaacacgaat caaaaagcga ttgagcgggt
     7381 tactgctttc cggcggaatt ttgccacgga tccagtaacc catcaggata gggatcacta
     7441 cgatcgccag cagcgccgca cccgccatcg catacgtttt ggtgaacgcc agcgggccaa
     7501 acagacgccc ttcctgccct tccagggtga agatcgggat aaacgacagc gtgataatca
     7561 gcagactgat aaacagcgcc ggccccactt caacagatgc attggtaatc acctgccagc
     7621 gcgttttatt atccagcgtg gcgtcaggat gctggtgctg ccactcttcc agccgtttat
     7681 gtgcattctc gatcatgacg atagcggcat cgaccatcgc cccgacggca atcgcaatgc
     7741 cacccaacga cataatattg gcattcagcc cctggaagtg catgacaata aaagcaatac
     7801 acaaccccag cggcaacgaa ataatcgcca ccagcgccga gcgcacatgc cagagaaaca
     7861 gcgcacagac taccgccacc acaataaact cttccagcaa cttgccgctg aggttgtcga
     7921 tggcccggtc aatgagctgg ctgcgatcgt atgttgtaac tatctccacg ccttccggca
     7981 gactgctttt cagcgtttcc agtttgtcct tcacggcggc gatcacttca cgggcgtttt
     8041 tgccggagcg taggatcacc accccgccca caacttcgcc gttcagttcg gcaatgcccc
     8101 ggcgcatctc cgggccaacc tggatcttcg caacatcacg cagataaaca ggcacgccat
     8161 tttcactggc ttttaaaacg atgtgattaa agtcgtcgag cgtttgtaga tagccgctgg
     8221 cgcgcaccat atattctgct tccgccagtt cgattgacga accgcccgct tcctggtttg
     8281 aagcatccag cgcgcttttt acttcggcga gactgatgcc atactgcgcc aggcgctgag
     8341 gatcgataac cacctgatac tctttcacca cgccgcccac cgacgccact tccgcaacgt
     8401 cagggatggt ttttagctca tatttgagaa accagtcctg caatgagcgt aaatcggcca
     8461 gatcgtgctt accgctgcga tccaccagtg catattcata gatccagcca acacccgtgg
     8521 catctggtcc cagctcggcg ctgactcccg caggcagctt accctgtacc tggttgaggt
     8581 actccagcac ccgcgaacgc gcccagtacg gatcggtacc atcttcgaaa atgacataca
     8641 cataagagtc gccaaactgc gagaaaccac gcacagtctt cgcgccaggc accgacaaca
     8701 tggtggtagt tagcggataa gtcacctgat tttcaacgat ttgcggtgcc tgaccgggat
     8761 agctggtttt aataatcacc tgcacatcgg agagatccgg caacgcatcc actggcgtgt
     8821 taatgatggt ccaggtgccc cagatactca gaaataacgc gcccatcagc accaaaaaac
     8881 ggttcgccac cgagcgacga ataatccatt caatcattgg ttattccctc aatgcgcatg
     8941 ggtagcactt tcagagcgca tccgctccag tgcgccagaa atattggctt cggaatcaat
     9001 caggaacagg ccgctggaaa ccaccttttc accttccgcc agaccagagc gtaatgcggt
     9061 gacaccttgc gatgcctgga aaacggcaac gcgttttggt acaaagcgcc cgtcggcatc
     9121 aacggtaatc acccgctgtt cgctgccggt atcaatcagc gcctgtgacg gaatgagcag
     9181 catcggttcg ctggcggtat tgagttgcaa ccaggcgttc attcccggtt ttagcgcctc
     9241 gtcggcgttg tcgacttcca gacgcagctg cagcgtgcgg gtcgcggcat ccacgccagg
     9301 tagtagcgtc catttgcgga tggtgagtgt tttatcaggt cgcgccggaa cggtgagcgt
     9361 aaactgcgag gcatctttca ccagccaggc gatggactcc gggatcgcag cagtgaccca
     9421 caccgggtcc ataccctgaa ttttcgctac cacgttatct ttggcgatat tcattcccgc
     9481 gcgcagatca aacgcggtga tcacgccatc aatgggtgct ttgagcgtaa agcgagtctg
     9541 gattttttgc gtggcgatca ggcggcgaat atccgcctcc ggcattcccg ccagtcgcag
     9601 ccgctcaaga atgccttcgg tctgggtcgc cgtaccgccg gtttcgcgca gcagtaaata
     9661 ctcactctgc gcttccaccc agtcgggaat ggtcaggtcg agaagcggcg cgcccttttg
     9721 tactttatcg cccacggtaa gcggatacac cttgtcgata aacccggcgg cgcgagcctg
     9781 cacaatggca tactgatact cgttgtaact gacattcgcc gggaaactct gggcaaaagt
     9841 cagcggcccg cgcgtgacgg tagccgtttt cacccccaga ttctgcgtct gagtcgggtc
     9901 aatgcgcaca ccagacgcag aactctcttc atcggcatat ttcggcacca gatccatatc
     9961 cataaacggc gatttacctg gtttatcgaa ccgcgtattg ggatacatcg ggtcgtacca
    10021 gaataagatt ttacgttctg cggtcgacgt tttttctgca ggcggttccg cctttgcaaa
    10081 ccaggtaaaa cctgccgcag aaataatacc gcccgcgatc atgctgccga taataagcgc
    10141 gatttttttc atctcattaa acctgggtta ctggctgact ttaatatcct gtaataaaga
    10201 aaggttgccc tgctggacaa aattaaacgc cactttgtcg ccggttttaa ttccactcat
    10261 tttcgtctgc ggggtgatgg taaagcgcat ggtcatctcc ggccagttca cggcagcaat
    10321 cggatcgtga tggatggtga tttttttgct ttccagatca aaacctttta ccacgccggt
    10381 ggcactaatg acctgtggtt gtgcttcgct catggtttca tgatgatgtt cgttagcctg
    10441 ggcattaaag ccaataacgg taaacagact gaacattgcg acttgcagtg cttttttcat
    10501 ttattttctc ctggagttaa aaattattgc tgccaaccgc caccaagtgc ggtatataaa
    10561 gaaatttcgt tgacctgacg ggcataattc aggtcaagta cggtttgtcg ggttgcaaat
    10621 aaagaacgct cggcatccag cacttccaga taacttactg cgccgtgctg atataatgcc
    10681 cgcgcccgtt gcaaagtaat ttgcagcgat gccagataac gctgctgggc gctaatttga
    10741 tcgttcaggc tttgacgtaa tgccagcgca tctgccactt ctttaaaggc gttctggatt
    10801 ttctgttcat aattcactac cgactgctgc tggcgaattt cggcgatatc cagattggcc
    10861 tggttgcgtc cggcattaaa aatggggatc tcaattttag gaataaaatt ccacatcccg
    10921 ctgctggcgt taaataatga cgacagatcg ctgctggcgg ttgatattcc gctggtcagg
    10981 ctgatagacg ggaaaaatgc cgcgcgtgca gcaccaatat tggcattagc cgccattaac
    11041 gcatgttcag cttccataat atccgggcgc tgcaataaga tttgcgacga caagcccgcc
    11101 ggtaatttaa cgctttgcag gctgtcgctg tttactgtct gcgcttgcgg cagcttgccg
    11161 tagcttccca gtaacagttg caatgcatta ttcgcctgcg ccagttcccc ctgacgttta
    11221 gcgatgtcgc tgcgggtact ttcaatcacc ccgcgagcct gttccagcgc cagaacatta
    11281 ctgctacctg tcaacagttg tttttcgaca aacgcatatg actgctgata attacgcagc
    11341 gtttcttcgg ctatttgcaa ttgcgcatac gccagctgct gattgaaata gctttgcgcg
    11401 acattagaaa ccagcagaat atgcaccgcg cgctgagctt cctcagtggc taaataattt
    11461 tgtcgctcgg cttcgctcat gttctttaag cgcccgaaaa aatcgaggtc aaagctggcg
    11521 ttaaggccag tagaaaactc ccgcgtcgtg gctgaatcgc ctttaagatt gccgctccag
    11581 ctgccgctgc cctcaccatt gagctgtggg tagcggtcgg catcagtcag acgatattgc
    11641 gcccgtgcct cctgcacttt cagcgtcgcc atgcgcaaat cccggttatt ctccagcgcc
    11701 tcgctaatca gcgtcttcac ctgattatca acaaaaaagg tgcgccagcc cgcgttctga
    11761 tagttatctg ctgcgttaac caggccgttc tggctgagtg agaactgctg cggtacgggc
    11821 attgccgggc gctgataatc cggtgccagt gaacaaccgg ttagcgcaag ggccacacaa
    11881 aatggcagaa gtttacaagg agacataggc tcataatttc tggtgatttt ataccgccaa
    11941 ctttactcgc caggctctga ttttccggtg acaggaaaat gacaaaattg tcattttgcc
    12001 aataagcgat tgccatctga tcccgctact ctagaattgc ccgggcaaca tgcggaggaa
    12061 atatgaaact gttgattgtc gaagatgaaa agaaaaccgg agaatacttg accaaagggt
    12121 taaccgaagc cggttttgtg gtcgatttgg ccgacaacgg gctgaatggc taccatctgg
    12181 cgatgaccgg agattatgac ctgataatcc tcgatattat gctgccggac gtgaacggct
    12241 gggatatcgt gcgcatgttg cgctccgcca ataaagggat gccgattctg ttgcttaccg
    12301 cgcttggcac cattgaacat cgcgtcaagg ggctggagtt gggggccgat gactatctgg
    12361 tgaagccgtt cgcgtttgct gaactgctgg cgcgggtgcg cactctcctg cggcgcgggg
    12421 cggcggtgat tatcgaaagt cagtttcagg ttgccgattt gatggtcgat ctcgtcagcc
    12481 gcaaagtcac ccgcagcggc acgcgcatca ctctgaccag taaagagttt actctgctgg
    12541 agttttttct ccgccatcag ggcgaagtgc tgccccgctc gcttatcgcc tcgcaggtat
    12601 gggacatgaa ttttgacagc gacactaacg ccattgatgt ggcggtgaag cggctacgcg
    12661 gcaaaatcga caacgacttt gagccgaagc tgattcaaac cgtgcgcggc gtgggttaca
    12721 tgcttgaggt gccggatggt cagtaagcca tttcagcgcc cgttttcgct ggcaacccgc
    12781 ctgacctttt ttatcagcct ggccaccatc gcggcgtttt tcgcctttgc atggatcatg
    12841 atccactcag taaaagtgca ttttgccgag caggatatta atgatttaaa agagattagt
    12901 gccacccttg aacgcgtact aaatcaccct gacgaaacgc aagcccgacg cttaatgacg
    12961 ctggaagata tcgtcagtgg ttattccaat gtgttgattt ccctggcaga tagccacggt
    13021 aaaacggtgt atcactcccc cggtgcgcct gatatccgcg agtttgcgcg cgatgccata
    13081 cccgataaag acgcgcgggg cggcgaggtg tttctcctct ccggcccgac gatgatgatg
    13141 ccaggccacg gtcacgggca tatggaacac agcaactggc ggatgatcag tttgccagtt
    13201 ggcccactgg ttgacggcaa accgatttat acgctctaca tcgcgctttc gatcgatttt
    13261 catctt
//
LOCUS       DBFIFI010000119        13261 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.119, whole genome shotgun sequence.
ACCESSION   DBFIFI010000119 DBFIFI010000000
VERSION     DBFIFI010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.119"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            48..761
                     /gene="ydiV"
                     /locus_tag="N7B39_003189"
     CDS             48..761
                     /gene="ydiV"
                     /locus_tag="N7B39_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HGU2423123.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQVLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            824..2260
                     /gene="selO"
                     /locus_tag="N7B39_003190"
     CDS             824..2260
                     /gene="selO"
                     /locus_tag="N7B39_003190"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HGU2423124.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPDKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLNDYEPGFICNHSD
                     HQGRYSFDNQPAVALWILQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(2264..2455)
                     /gene="hemP"
                     /locus_tag="N7B39_003191"
     CDS             complement(2264..2455)
                     /gene="hemP"
                     /locus_tag="N7B39_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HGU2423125.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRISSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(2587..3633)
                     /gene="aroH"
                     /locus_tag="N7B39_003192"
     CDS             complement(2587..3633)
                     /gene="aroH"
                     /locus_tag="N7B39_003192"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HGU2423126.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRINHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(3790..4623)
                     /gene="ppsR"
                     /locus_tag="N7B39_003193"
     CDS             complement(3790..4623)
                     /gene="ppsR"
                     /locus_tag="N7B39_003193"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HGU2423127.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            4956..7334
                     /gene="ppsA"
                     /locus_tag="N7B39_003194"
     CDS             4956..7334
                     /gene="ppsA"
                     /locus_tag="N7B39_003194"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HGU2423128.1"
                     /translation="MSNNGSSPLVLWYNQLGMKDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(7391..9036)
                     /gene="fadK"
                     /locus_tag="N7B39_003195"
                     /pseudo
     CDS             complement(7391..9036)
                     /gene="fadK"
                     /locus_tag="N7B39_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
     gene            complement(9093..9386)
                     /locus_tag="N7B39_003196"
     CDS             complement(9093..9386)
                     /locus_tag="N7B39_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HGU2423129.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(9383..10672)
                     /locus_tag="N7B39_003197"
     CDS             complement(9383..10672)
                     /locus_tag="N7B39_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HGU2423130.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDILVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAASAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLADIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(10728..11666)
                     /locus_tag="N7B39_003198"
     CDS             complement(10728..11666)
                     /locus_tag="N7B39_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HGU2423131.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSSLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(11686..12450)
                     /locus_tag="N7B39_003199"
     CDS             complement(11686..12450)
                     /locus_tag="N7B39_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HGU2423132.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYMVQDAQLEHALPLDTAKALA
                     AAVEKIGFDLLIFGKGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIGWSQSAPLAEL
                     TGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            12766..>13261
                     /locus_tag="N7B39_003200"
     CDS             12766..>13261
                     /locus_tag="N7B39_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HGU2423133.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENHLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENF"
BASE COUNT         3203 a         3292 c         3426 g         3340 t
ORIGIN      
        1 ccttttagcc ggatactgaa aaacatcctt cgagagggac ggttaccatg aagatttttt
       61 tggagaatct ttatcattct gattgctatt tcctgccgat aagagataac cagcaagtcc
      121 ttgtcggcgt tgagttaatt acccatttct ccagtgaaga tggcacggtg cgtatcccga
      181 cgagtcgggt tatagcgcaa cttaccgagg agcaacattg gcaattattt tcagagcagt
      241 tggaattact aaaatcatgc caacattttt ttattcaaca taaactcttt gcctggctta
      301 atttaacgcc acaagtggct acattattat tagagaggga taattacgca ggcgaactat
      361 taaaatatcc gtttattgaa ctgttgatta atgagaatta cccgcatctc aatgaaggaa
      421 aagataatcg cggtctttta tccttatcgc aagtgtatcc ccttgtgtta ggtaatttgg
      481 gagcaggcaa cagcacaatg aaagcggttt ttgatggtct tttcacccgc gtaatgctgg
      541 ataaaagttt tattcagcaa caaatcacgc atcgctcctt cgaaccgttt attcgtgcga
      601 ttcaggcgca aatttcccct tgttgcaatt gcatcatcgc ggggggcatt gatacgccag
      661 aaatattagc acagattacc cccttcgatt tccatgctct ccagggatgc ctgtggcctg
      721 ccgttcccat aaatcagata acgacgttgg ttcagcgata accctcctgt ttgctggtgt
      781 ttaagacgag agtaaccgtc tacactatca aacaggaggc tctatgaccc tgtcttttat
      841 tacccgctgg cgcgatgaat tgccagaaac ctatacagca ctttccccta cgcctttaaa
      901 taatgcccgg ctgatttggc ataataccga actcgctaac acgctgagta ttccatcgtc
      961 gctgtttaaa aatggcgcag gtgtctgggg cggcgaaacc ctactgcctg gtatgtcacc
     1021 actggcgcag gtttacagtg gtcatcagtt cggcgtctgg gcgggccaac tgggtgatgg
     1081 gcgcggcatt ttacttggcg aacaactgct tgctgatggc actacaatgg actggcatct
     1141 gaaaggtgct ggcctgacgc cttattcgcg aatgggtgat ggacgggcgg tattacgttc
     1201 gacgatacga gaaagtctcg ccagtgaggc gatgcattat ctgggcattc cgacgacccg
     1261 cgcgttaagt atcgtcacca gcgattcgcc agtgtatcgg gaaacggtgg agccaggcgc
     1321 gatgctgatg cgtgtggcac caagtcatct gcgctttggt catttcgaac atttttacta
     1381 tcgccgcgag ccggataagg ttcgtcagtt ggctgacttt gccattcgtc attactggtc
     1441 acatcttgaa gatgatgagg acaaataccg tctctggttt aacgatgttg tcgcacgtac
     1501 cgcatcgtta attgcccaat ggcagacggt cggctttgca catggggtga tgaataccga
     1561 caacatgtcg ctgctgggac tgacgcttga ttacgggccg tttggttttc ttaatgatta
     1621 cgaacccggt tttatttgta atcactcgga tcatcaagga cgttacagct ttgacaatca
     1681 acctgctgtc gcgttgtgga ttttacagcg tctggcgcag acattgtcac catttgttgc
     1741 cgtagatgcc ctgaatgagg ccctggacag ctatcagcag gttttgttga cgcattatgg
     1801 acaacggatg cggcagaaac tgggctttat gacggagcaa aaagaggata acgcgctact
     1861 gaatgaatta ttcagtctga tggcgcgaga gcgcagcgat tatacccgca catttcgcat
     1921 gctgagtctg accgagcagc acagcgcggc gtcaccgcta cgtgatgagt ttattgatcg
     1981 tgcggcattt gatgactggt ttgcccgtta tcgagggcgt ttgcaacaag acgaggttag
     2041 cgatagtgag cgtcagcaac tgatgcaaag cgttaacccc gctctggtat tgcgcaactg
     2101 gttggcgcaa cgggcgattg aagcggcaga aaagggtgat atgacggaat tgcaccgcct
     2161 gcatgaggcg ttgcgaaatc ctttcagcga cagagatgat gactatgtca gtcgtccacc
     2221 tgactggggt aaacggctgg aagtcagctg ctcgagttaa aggctacttg gtcaacagca
     2281 gcttgccagc ctgagtttta cggagcagat attcttgccc gtcatgatcg ataatcagtt
     2341 tgccatccgg acctaacagt gtctggctgg aaattcgccg aataggctgc ggggaagcgg
     2401 tcttgtgatt ggcatccgtt tcaggcgtga gttttctgct atccgtataa cgcataaaaa
     2461 ttaataataa ttgatataac aatgattctt attatcaata ttatggctga ccagagcaag
     2521 cagtaatgtg ttgagaagac aacaagttgc gcagaaatgc ccatcccatc aggaatgggc
     2581 acgcattcag aagcgggtat ctaccgcaga ggctagtttt tcgaccaggc gttcggtatc
     2641 ttcccagccc agacacgggt cggtaatgga ttgaccgtaa gtgagcggct gaccgccgac
     2701 gattttttgc gttccttcgc gcaggaaact ttccgccata attccagcaa tcgccgtaga
     2761 gccattgcgg atttgctgac aaatatcctc acaaacttct aactggcgac ggtgctgctt
     2821 ctggcagtta ccgtggctga aatccaccac cagatgttca ggtaaatcaa actcgtgcag
     2881 cgtatcgcag gctgcggcga tatcatcggc atgataattc ggttttttgc cgccacgcat
     2941 aataatgtgg ccatacgggt tgccgctggt ctgatagatg gtcatctgac catttttgtc
     3001 tggcgagagg aacatatggc tggcgcgggc tgcgcggata gcatccacag caatccgcgt
     3061 attgccatcg gtaccatttt taaaacctac cggacaggag agtgccgaag ccatttcacg
     3121 gtggatctga ctttcggtag tacgtgcgcc aatcgcgccc caactgatta aatcagcaat
     3181 aaactgaccg gtcaccatat cgaggaactc agtcgcggtt gggacgccca gctcatttac
     3241 ctgtaaaagt aatttgcgcg ccagctccag accgtgattt atccgatagc tgccgtttaa
     3301 atctggatcg gagattagtc ctttccagcc gacaacagtt cgtggttttt caaaataggt
     3361 gcgcattacg atttccagcc gtgactggta ctggttgcgc agcgactgca gacgggtggc
     3421 gtactccatt gcagcggtga gatcgtggat cgagcagggg ccaataatga ccaacagtcg
     3481 cttatcttca ccattgagta ttttttcaat tctgcggcgg gagtcggtga catgggtggc
     3541 gacgccaggc gttacgggat accgtagcgc gagttcggcg ggcgttacca ggctctcaat
     3601 acgcgcagta cggagttcgt cagttctgtt cattacaagt ctcagaaatt tgttgcttct
     3661 acggcaggac tgccgggaag tgatgcgcat cacgataaac caatcgctgc tgatttcaag
     3721 tgcgaggtgt gtcacacctc gccgggtggt tagcatgata acaaaaaaat aagctaacgc
     3781 actagttctc tagtacattc ggcgactaag gcccatgata tcgaggatct tggtggcaat
     3841 ctcttctacc gaataattgg tactgttaat ccacgggatc tgatttttac ggtacaaggc
     3901 ttccacttcc gcgacttcca tcctgcactg acgaagcgag gcatagcgac tgttctcccg
     3961 acgttcctcg cgaatcgccg ccagacgttc cgggtcgata gtcaggccga acaatttatg
     4021 ctgaagcggt ttgagcgacg cgggtagcac cagattatcc atatcgtcgg caataaaggg
     4081 gtagtttgcc gcgcggatac caaattgcat tgccagatac agactggtgg gggttttacc
     4141 acagcgagaa acaccgagca ggatcacctg agcctgatcc agattgcgca acgaaatgcc
     4201 gtcatcgtgg gcgagggtgt aatcaatcgc cgcaatgcgc gcatcatatt tattgagatt
     4261 attagggtta aggccatggg tacgatgagc aatcggcgtt ggatccagtt tcatctcttg
     4321 ttgtagcggg gcaaccagcg cctgaacgat atcctggcaa aaaccttcac tttgcaagat
     4381 gatggcgcga atttccggca acacgatgga gtagaagacc agcggacgca cgcctgtctg
     4441 gtgataaatt gcgtcaatct gatccttcac tgcccgtgca cggctctcat tttcgacaaa
     4501 cggcagcgtg atgctgctga tagtgacggg aaattgtgac attactgcgt gtcctaatac
     4561 ctccgcagtt attgccgtac catcagaaat ataaaaaacg tggcgatcaa cagcattatc
     4621 cattttgttc ttcccgtgat gcagacataa ttctatgaaa gcataaatta aaaacgcaca
     4681 gaagcgtaga acgttatgtc tggtttataa aatgaacctt caattttatt ttttatgaaa
     4741 acagcatttc atttttatgg tttcgtttat accgatggtt tatgtggaaa ttgtcgaaaa
     4801 gagcagattt gcgcaacgct gggatcagtc ttaacaagta aaaaaatata tttgcttgaa
     4861 cgattcaccg tttttttcat ccggttaaat atgcaaagat aaatgcgcag aaatgtgttt
     4921 ctcaaaccgt tcatttatca caaaaggatt gttcgatgtc caacaatggc tcgtcaccgc
     4981 tggtgctttg gtataaccaa ctcggcatga aggatgtaga cagggttggg ggcaaaaatg
     5041 cctccctggg tgaaatgatt actaaccttt ccggaatggg tgtttccgtt ccgaatggtt
     5101 tcgccacaac cgccgacgcg tttaaccagt ttctggacca aagcggcgta aaccagcgca
     5161 tttatgaact gctggataaa acggatattg acgatgttac tcagcttgcg aaagcgggcg
     5221 cgcaaatccg ccagtggatt atcgacactc ccttccagcc tgagctggaa aacgccatcc
     5281 gcgaagccta tgcacagctt tccgccgatg acgaaaacgc ctcttttgcg gtgcgctcct
     5341 ccgccaccgc agaagatatg ccggacgctt cttttgccgg tcagcaggaa accttcctca
     5401 acgttcaggg ttttgacgcc gttctcgtgg cagtgaaaca tgtatttgct tctctgttta
     5461 acgatcgcgc catctcttat cgtgtgcacc agggttacga tcaccgtggt gtggcgctct
     5521 ccgccggtgt tcaacggatg gtgcgctctg acctcgcatc ttctggcgtg atgttctcca
     5581 ttgataccga atccggcttt gaccaggtgg tgtttatcac ttccgcatgg ggccttggtg
     5641 agatggtcgt gcagggtgcg gttaacccgg atgagtttta cgtgcataaa ccgacactgg
     5701 cggcgaatcg cccggctatc gtgcgccgca ccatggggtc gaaaaaaatc cgcatggttt
     5761 acgcgccgac ccaggagcat ggcaagcagg ttaaaatcga agacgtaccg caggaacagc
     5821 gtgacatctt ctcgctgacc aacgaagaag tgcaggaact ggcaaaacag gccgtacaaa
     5881 ttgagaaaca ctacggtcgc ccgatggata ttgagtgggc gaaagatggc cacactggta
     5941 aactgttcat tgtgcaggcg cgtccggaaa ccgtgcgctc acgcggtcag gtcatggagc
     6001 gttatacgct gcattcacag ggtaagatta tcgccgaagg ccgtgctatc ggtcatcgca
     6061 tcggtgcggg tccggtgaaa gtcatccatg atatcagcga aatgaaccgc atcgaacctg
     6121 gtgacgtgct ggtcactgac atgaccgacc cggactggga accgatcatg aagaaagcat
     6181 ctgccatcgt caccaaccgt ggcggtcgta cctgtcacgc ggcgatcatc gctcgtgaac
     6241 tgggcattcc ggcggtagtg ggctgtggtg atgcaacaga acggatgaaa gacggtgaga
     6301 acgtcactgt ttcttgtgcc gaaggtgata ccggttacgt ctatgcggag ttgctggaat
     6361 ttagcgtgaa aagctccagc gtagaaacga tgccggatct gccgttgaaa gtgatgatga
     6421 acgtcggtaa cccggaccgt gctttcgact tcgcctgcct gccgaacgaa ggcgtgggcc
     6481 ttgcacgtct ggaatttatc atcaaccgta tgattggcgt ccacccacgc gcactgcttg
     6541 agtttgacga tcaggaaccg cagttgcaaa acgaaatccg cgagatgatg aaaggttttg
     6601 attctccgcg tgaattttac gttggtcgtc tgactgaagg gatcgcgacg ctgggtgccg
     6661 cgttttatcc gaagcgcgtc attgtccgtc tctctgattt taaatcgaac gaatatgcca
     6721 acctggtcgg tggtgagcgt tatgagccag atgaagagaa cccgatgctc ggcttccgtg
     6781 gcgcgggacg ctatgtttcc gacagcttcc gcgactgttt cgcgctggag tgtgaagcag
     6841 tgaaacgtgt gcgcaacgac atggggctga ccaacgttga gatcatgatc ccgttcgtgc
     6901 gtaccgtaga tcaggcgaaa gcggtggttg aggaactggc gcgtcagggg ctgaagcgtg
     6961 gtgagaacgg gctgaaaatc atcatgatgt gtgaaatccc gtccaacgcc ttgctggccg
     7021 agcagttcct cgaatatttc gacggcttct caattggctc aaacgacatg acgcagctgg
     7081 cgctcggtct ggatcgtgac tccggcgtgg tgtctgaact gttcgatgag cgcaacgatg
     7141 cggtgaaagc actgctgtcg atggcgattc gtgccgcgaa gaaacagggc aaatatgtcg
     7201 ggatttgcgg tcagggtccg tccgaccacg aagactttgc cgcatggttg atggaagagg
     7261 ggatcgatag cctgtctctg aacccggaca ccgtggtgca aacctggtta agcctggctg
     7321 aactgaagaa ataaaataaa tccccggcgg cgttttgtcg ccgggtttat gtgatccccg
     7381 aagatgaaac ttattcaatc tcttcacaga catcctgcgt taatcgccgc ataatatctt
     7441 tccgtaacaa aaacttttgt attttacctg aggcagtgcg cggtagtttt tcgattacca
     7501 cgatatgttc aggatattta tattttgcga cccgtttacg gctaaaaaag ccaccacctc
     7561 ttccagcgat aatgaatgat gcggcgcttt cagcacgaca taagcgcatg atcgttcacc
     7621 taaacgttca tcgggcattg caaccacaca ggcatcgtga attttaggat gctgcaataa
     7681 aatatcttcc acttcacggc tgctaatatt ttcgccgccg cggacaataa tatctttttt
     7741 gcgtccggta atttttatat agccagcctc atccatacgg cagagatcgc cgctgtaata
     7801 ccagccttct tcatccaggg cacgggcggt taattcaggt tcatcaaaat atcccataaa
     7861 cacattgggg ccacgcgagg cttcttcacc ttcgcaacct ggcggtaagg tcttgcgtgc
     7921 gtcatcgacc actttaatct ctacacctgc ggcagcgtaa ccatcggtgt gcataaagcg
     7981 cgacaaagga tcatcgagat tcaccaccgc atgcggcgaa ctttctgtgg aaccataaac
     8041 acttaataat ttaatgccgc gctgctggca ttcacgcgcc acttttttgg ggattgtggt
     8101 accgccgcaa agaaagaagc gcagcgctga aaggtctgcg ggttgtttct ctagtaaatt
     8161 caaaagatca tagacaaacg gcgttgcgcc gagcatacag gagcaacgct gctgctcaag
     8221 cagcgcgaga cacgcatcag gagtgaaaat atctaacaac acgctgcgag cgccaattaa
     8281 gaatggtgcc gttacgccat gcagaaagcc cgttgcgtga ccaagtggcg caggcatcat
     8341 aaagacatcc tgccaggtta gattcagccg cgcgcaataa gcccgctcac tggcgagaat
     8401 attgttatgc gtaagcatca cgccctttgg cagaccctcg gttccggagg taaacagcac
     8461 cgcagctaat tcatcgccgt gggtcgttat cgccgtggtc agtggggtat tgtcggcgat
     8521 aatctgactt aatgagaggg aagaggtggc gggagccagt ttgtccacgc cgacaatttg
     8581 ttgtagttgt ggaagctgat tttgcagcgg caggattaaa tctaccggac gcgtttgttt
     8641 aaacaacgtc ggtgtaaaga acatttttgc ctgacactta ttaagcaccc ataccagttc
     8701 tgcttcccgc caggaaggca acagcggtac ggaaaccgca ccgattttca ggcaggcaag
     8761 atagataacg gtaaattcac accagcctgg cagttgaaat gctatgcgat cgcctgattc
     8821 aatacccttc gctaacatcc agtttgccag acagctcgcg gcgtgatcga gcgcgctata
     8881 ggtgtacgat gctccatgat tatcgaccac ggcaattttg tctggcatcg cacgagcggt
     8941 ctgctgccag taatcggcca gcgaagcatc gccccatagc ccttgctgac gatacgccgc
     9001 acgacgttgt tcgttaaacg ttaatgtcac tttcattttg gactctcgaa acagaacatc
     9061 aggcccgaga tgcgggcctg tgggatgcat gattagccgt agcgaaaatc aataccgaag
     9121 gtgcctgcgg gatattgcca ctgttcgaga atagtgttac cgcacagcac ccgacaggtg
     9181 ccgcactcca gacaaccggc ggaatcaaaa tgaatgtttc ctgcgtcatc ctgcttataa
     9241 agtccggcag ggcaggcttt cattaattta cggaattcat tgatatcggg atttgccgcc
     9301 aaaatgatat gcgggtggcc ctcatcaaca tggaatttat tgacgcctaa tttgatgtcg
     9361 acgttaaccg tagcgttctg gctcatagcg cggttgctcc cttaatgcca tctttcagca
     9421 agttgatcag cccaattttc ttcgcgtgtc ccatgatcat tttgcgtacc ggctggttag
     9481 gcttaccgtc aatggtgaac atctcgttca tgatgtcggc taccattcgt gggtattggc
     9541 taaacaggcg cgggttttcc atcagcgccg ggatcttgcg aaaatgctgc atatcacgca
     9601 tgacgcagct ttgttccagc tcacgtttgt attgcgccag actgctggcg gagaaatccg
     9661 cgcgttcttt ggcggcgatc actgttgtgg cggcagcctg agccgatgca atggctaaat
     9721 ccatgccgcg gaccgtaaaa cccaaattca ggcagaagcc tgcggcgtca ccaacgatca
     9781 tcacgccatc gttaaccagt tgcggtacca tcgccagacc gccttctggc accatatgcg
     9841 cggaatattc aagcagtttg ccgccgctaa tcagcgggcg aatggcgggg tgttgtttaa
     9901 aatcttccag catttgcggc acgctttttt gcgcatgggc gatatcagcc aatccacaaa
     9961 ccagccccaa ggatatggaa tccttgttgg tatagagaaa tcccccgccc atcaggccgt
    10021 cagaagggga accggcaaac agccaggcgg caccttcctc gcccgtaata ttaaagcgat
    10081 cgttgatctg ttctggtgtg aggccaataa cctctttaac accaacagcg taatgatgcg
    10141 gatcggaagc gggaaccatt cccagcgagc ggccaagcat cgagttaacg ccatcggcta
    10201 aaatcaccac attggcttcg agaatatcat ccccggcctg gactccagtg accttgtttc
    10261 cttcacgaac caacgcatcg acgcgaactc ccgggataaa ctgtgcgccc gcctgctcgg
    10321 cttgttccat caaccacggg tccagacgat tacgcaatac ggtataagat gcgtgttgcg
    10381 gaacatctgg ttgctcgcgg tgaaaatcga gggtaacggc gctctcttcg gttaagaagg
    10441 agattttctc gcgtgtgacc ttgcgttcta ccggcgctga tgctgcaaag cctggaatga
    10501 ttgcttcaag tgtgtgggca taaagacgcc cgccggtcat gtttttacat ccggcactgt
    10561 cgccgcgttc tatcaccagg atatccagcc cggctcgcgc catgaccagt gcggcaacgc
    10621 taccagcaac gcccgcaccg accacaatgg catcaaattt gtcatccgac atgcgttatg
    10681 ctcccttccc gctgcgttta cagcgggaga tgtggttgaa aggggaatta gcggctcaac
    10741 tggctaatca gggcagggac gactttgtag atatcgccca ccagaccgta gtcggcatag
    10801 ttgaagattg gcgcattttt atctttattg atggcgacaa tcacttttgc gccgttgccg
    10861 ccaaccatat gctggatctg cccggagatc cctagcgtca ggtagagatc ggatttcagc
    10921 aacacgccgg agacaccgat ataacgttca cgctccatcc agttctcgcc ttcggcaatt
    10981 ggacgtgaac agccgacttc agcattcagc accgccgcca gttcgtggac cattttcagg
    11041 tcatcctgcg ctgccagacc acgaccgacg ccaaccacac gttttgcttt gctcaggtcc
    11101 acgctgcttg cggctttcgc acggcgttcg cgacagagaa tttcatgacg cggagcaacg
    11161 taaggtaccg tttctgtcgg gcactgatga gaggtatcac tggtgcacgg ttcctgaaca
    11221 ccgggtgcaa gggtaatgat cgccagcgag ctgttgatct tttcctgagc gaacgctaac
    11281 ccgccataca tccggtgttc ggcgcaaatg tgaccatcga caatatccac cgccgtggca
    11341 tcgttcacca gcgccgcatt cagttgcaca cttaaccgtg ctgccagcgc tttaccacgt
    11401 ttcgtcgcgg ccagtagcaa catagcgggg tgtttatctt tcagcagagc agcaatgctt
    11461 tcggcgtaat tttcagtgcg ttgcagcgcg tcgttttgct caagaacata aatacatttt
    11521 ggaccataag gcataactgc ctgcgcctgg tcggtatttt gtacaatggc atacacctgt
    11581 tggccccatt gctgagcgcc gccaaacagt tcagcataac gttcaggatt atcgctaaag
    11641 acccagacgc tgtttaattg actcatcgtt tcttctccgt aggcttcagt tcagggcttt
    11701 cttcagatgt tccgccagct cggcaatggc ctccggcgaa tcgttatcga gaatgatgtg
    11761 cttacgttct gtttgcggcg gtacgcgaat gccggtcagt tcagcaagtg gtgcgctctg
    11821 gctccagcca atatcacttg cctgccactg atttaccggt tttttacccg cgccgagaat
    11881 ggctttcatc gaaggaatgc gtggcacgtt aatatcggag gtgacgcaga gcacggctgg
    11941 aacagagagt tcaataactt caacgtcatc ttcaagcgtg cgttcaatca ccagtgtatt
    12001 gccctgacgc tgaatagcac tcactgcatt aatcaccgga agttgcagaa tttctccgac
    12061 cagcaagcca acctgctggg cataaaggtc gccggaacct ttaccaaaga tcagtaaatc
    12121 gaagccgatc ttttcaactg ctgccgccag cgcttttgcg gtatcgagag gcagtgcatg
    12181 ctcaagttgt gcatcctgca ccatatacag gctgtgcggc ccgcgggata gcacgtcttt
    12241 gcgcactttc gagttctgca acaatgagcc gccaacggtc agcgcggcta tctcatcgtc
    12301 atctgttgca agctggcttg cagcttcaat ggcattgaga tcgaactggc tgattttggc
    12361 gtcggcattg tcgaaattca gggtgtattc tggagtgaca acaatgtcct gttcttcagg
    12421 caccagctta aagcaggtta ttattttcat gatttctcct gtgagtcgca tacggaacca
    12481 tctccgatgg aaggtaaaaa tggcctgaaa atagtgtgcg atagcggtgc ggggttttcg
    12541 acggagcaag ctgttttcct tgccaacaat cttgcaaaaa aaatgcgatc gctaaaacaa
    12601 aagtgcaatt tgcggcaata tagttattcc atgaccgtac aatcttgcat tcagtgtaaa
    12661 taatcttgtt tagctctcgc taaacgcggt tgtataaccg caatacacgc cgtattttat
    12721 ccggcatata ttgtttttgc ttgctcatgg aatagggagg ttgtcatgta tcaacgctgt
    12781 tttgataatg ccagtgaaac gctgtttgtc gctggtaaaa cgccacggct ttcacgtttt
    12841 gcatttagcg atgatccaaa atgggagtct ggacatcacg ttcatgacaa tgaaaccgag
    12901 ctgatttacg tcaagaaagg ggtggcaagg tttaccatcg attcttcgtt atatgtcgcg
    12961 catgcagatg atatagtggt gatagaacgc ggcaggctgc atgcggtggc ctctgacgtt
    13021 aacgatccgg caacgacgtg tacttgtgcg ctgtacggct ttcagtttca gggggctgag
    13081 gaaaatcatc tactgcaacc gcattcttgt ccggtaattg ccgcggggca gggaaaagaa
    13141 gtcattaaaa ccttatttaa tgagctaagt gtgattttgc cgcaaagtaa aaatagccaa
    13201 acatcttcgt tatgggatgc atttgcctat acattagcaa ttctttacta cgaaaacttt
    13261 a
//
LOCUS       DBFIFI010000120        12884 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.120, whole genome shotgun sequence.
ACCESSION   DBFIFI010000120 DBFIFI010000000
VERSION     DBFIFI010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12884
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.120"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            235..1332
                     /gene="mltA"
                     /locus_tag="N7B39_003201"
     CDS             235..1332
                     /gene="mltA"
                     /locus_tag="N7B39_003201"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HGU2423134.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1571..2377
                     /gene="tcdA"
                     /locus_tag="N7B39_003202"
     CDS             1571..2377
                     /gene="tcdA"
                     /locus_tag="N7B39_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HGU2423135.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINSECCV
                     TVVDDFVTPDNVAQHMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2428..2871)
                     /gene="csdE"
                     /locus_tag="N7B39_003203"
     CDS             complement(2428..2871)
                     /gene="csdE"
                     /locus_tag="N7B39_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HGU2423136.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(2871..4076)
                     /gene="csdA"
                     /locus_tag="N7B39_003204"
     CDS             complement(2871..4076)
                     /gene="csdA"
                     /locus_tag="N7B39_003204"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HGU2423137.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGIVVIVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAENWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGMTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4268..4495
                     /gene="ygdI"
                     /locus_tag="N7B39_003205"
     CDS             4268..4495
                     /gene="ygdI"
                     /locus_tag="N7B39_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HGU2423138.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            4846..5763
                     /gene="gcvA"
                     /locus_tag="N7B39_003206"
     CDS             4846..5763
                     /gene="gcvA"
                     /locus_tag="N7B39_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HGU2423139.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5782..6177
                     /gene="ygdD"
                     /locus_tag="N7B39_003207"
     CDS             5782..6177
                     /gene="ygdD"
                     /locus_tag="N7B39_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HGU2423140.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            6170..7270
                     /gene="rlmM"
                     /locus_tag="N7B39_003208"
     CDS             6170..7270
                     /gene="rlmM"
                     /locus_tag="N7B39_003208"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HGU2423141.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7314..8045)
                     /gene="fucR"
                     /locus_tag="N7B39_003209"
     CDS             complement(7314..8045)
                     /gene="fucR"
                     /locus_tag="N7B39_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HGU2423142.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTG"
     gene            complement(8103..8525)
                     /gene="fucU"
                     /locus_tag="N7B39_003210"
     CDS             complement(8103..8525)
                     /gene="fucU"
                     /locus_tag="N7B39_003210"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HGU2423143.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8527..9945)
                     /gene="fucK"
                     /locus_tag="N7B39_003211"
     CDS             complement(8527..9945)
                     /gene="fucK"
                     /locus_tag="N7B39_003211"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HGU2423144.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIERLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGAEQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPLKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(10054..11829)
                     /gene="fucI"
                     /locus_tag="N7B39_003212"
     CDS             complement(10054..11829)
                     /gene="fucI"
                     /locus_tag="N7B39_003212"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HGU2423145.1"
                     /translation="MNKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(11862..>12884)
                     /gene="fucP"
                     /locus_tag="N7B39_003213"
     CDS             complement(11862..>12884)
                     /gene="fucP"
                     /locus_tag="N7B39_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HGU2423146.1"
                     /translation="FLYALGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFV
                     TVLGPESSGHFRLNLAQTFNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAY
                     KHSLVLSVQTPYMIIVAIVLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIR
                     HWRWAVLAQFCYVGAQTACWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGT
                     WLISRFAPHKVLAAYALIAMALCLISAFAGSHVGLIALTLCSAFMSIQYPTIFSLGIK
                     NLGQDTKYGSSFIVMTIIGGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFR
                     SQTATN"
BASE COUNT         3075 a         3221 c         3425 g         3163 t
ORIGIN      
        1 gagctaccag gctgctccac cccgcgtccg tggatgcgca ctatactccg ctcgcgtttt
       61 catgcaacct tttttttacc ccgatcacgg atctggcatg attttgaaca aaaacaaatc
      121 actaaagcct attttttgtg caaaatttgc gtcagggaga ggctgctcat ttcattaaac
      181 gcggtggttt gttatcttcg ttgcgcctta ttttttaacc tgaagaagag aacaatgaaa
      241 ggacgttggg taaagtacct tcttatgggc acggttgtgg caatgcttgc cgcctgctct
      301 tccaaaccaa ccgatcgcgg acagcaatat aaagacggga aatttaccca gcctttctct
      361 ctggtgaacc agccagatgc cgttggcgcg ccgattaacg ccggtgattt tgccgagcaa
      421 attaaccata tccgtaattc gtcaccgcgt ctgtatggca accagagtaa tgtttataac
      481 gcggtgcaag agtggctgcg cgcaggcggt gatacccgca atatgcgcca gttcggcatt
      541 gatgcctggc agatggaagg tgccgacaac tatggtaacg tgcagtttac aggctattac
      601 acgccggtaa ttcaggcgcg ccatacccgc cagggcgagt tccagtatcc tatttaccgt
      661 atgccgccaa aacgtggtcg tctgccgtct cgtgcggaga tctacgcggg tgcattgagc
      721 gataagtaca tcctcgctta cagcaactct ctgatggata atttcattat ggatgtgcag
      781 ggtagtgggt atatcgactt tggtgatggc agtccgctta actttttcag ctatgcaggg
      841 aaaaacggtc atgcctatcg cagcattggt aaggtgctga tcgaccgtgg cgaagtgaaa
      901 aaagaagata tgtcgatgca ggcgattcgt cattggggcg aaacacacag tgaagccgag
      961 gttcgcgagc tgctggaaca gaacccgtct ttcgttttct ttaaaccgca atcttttgca
     1021 ccggtgaaag gggcaagtgc ggtgccgctg gttggccgtg cttctgttgc ctctgatcgt
     1081 tccattatcc caccaggtac aaccttgctg gcagaagtgc cgctgctgga taataacggc
     1141 aaatttaacg gtcagtacga attgcgtctg atggtggcgt tggatgtcgg tggcgcaatc
     1201 aaaggccaac acttcgatat ttatcaaggg atcgggccgg aagccggaca ccgcgcaggt
     1261 tggtacaacc actatggacg tgtctgggtg ctgaaaaccg ccccgggcgc aggtaacgtc
     1321 tttagcggct gatgtgatat tctgtatgcc gtttttacgg atatgacagg gtgaggggta
     1381 acctcacatc ctgattattg actaagtgcg cgttgttcat gccggatgcg gcgtgaacgc
     1441 cttatccggc ctacaaatca tgcaaatcca aaatattgca ggagttatgt aggcctgata
     1501 agcgtagcgc atcaggcatt tatgcgttta gggtgaggga taacctcgcc ctttttaatt
     1561 ctgaggtgct atgtctgtgg taattagtga tgcatggcgt cagcgttttg gtggcacagc
     1621 gcgtctgtat ggtgaaaaag cgttgcaact gtttgctgac gcgcatattt gtgtggtcgg
     1681 tattggcggt gtcggttcct gggcggcgga agcgctggcg cgcacgggga ttggcgcaat
     1741 cacgcttatc gatatggatg atgtgtgcgt caccaatacc aatcggcaaa ttcatgccct
     1801 gcgcgataac gttgggctgg caaaagcgga agtcatggcg gaacgtattc gccagattaa
     1861 ctcggagtgc tgtgtaacgg tggtggatga tttcgtgacg ccggataacg tagcgcagca
     1921 tatgagcgtg ggttattcgt acgtgattga tgccattgat agtgtacggc ccaaagcggc
     1981 gctgattgct tattgtcggc gcaataaaat cccgctggtc acgaccggtg gcgcgggtgg
     2041 gcagattgac ccaacgcaga ttcaggttac cgatctggcg aaaacgattc aggacccgct
     2101 ggcggcgaag ttgcgtgagc gcctgaaaag cgattttggc gtagtgaaaa acagtaaagg
     2161 taagctcggt gtggattgcg tgttctctac cgaagcattg gtgtatccgc agtcagacgg
     2221 tacggtgtgt gcgatgaaag ccacggcgga aggaccaaag cggatggatt gtgcatctgg
     2281 atttggcgcg gcaacgatgg tgaccgccac ctttggtttt gttgcggttt ctcatgcgct
     2341 gaagaagatg atggcgaaag cggcgcgtca ggggtaaaca atcaggtcgg atgcgacgca
     2401 aacgtcttat ccgacccggt tctgtgctta aacctgcttc gtcgcagcga taatcgcctc
     2461 gcttaacgca tttaacccct ggctgcgtga ggcgctaagt tgcgcacgca atcccagctc
     2521 atcaaacaat gccagcggcg actgtgcctg caattcggcg gcggttttcc cctcaacggc
     2581 agtcaacagc accgccagga ggccgcgcac tatacgtcct tcgctgtcgc caaagaagtg
     2641 cattttaccg ttttcagcca cggtatatcc cagccagacg cggttttcgc atccggcaat
     2701 ctctttagcc tgcgctttta actcgtctgg caaagccgga agctgtttcc ccagcatgat
     2761 caactggcga tatttatctt cccattgcgt caacggtgcg aaggtattgc gtaacgtttc
     2821 tgcggttacg gttgtgccga acggatgtcc ggcgaattgc gggttcgtca ttaatccacc
     2881 aataattcca gcgcgcggtc aacggcattc accagcgcat ccacatcact ctttgtatta
     2941 tatggcgcaa aagaggcgcg cagtgtgccg gtcatgccta attctgccag tagcggctga
     3001 gcgcaatgtt gcccggcccg caaggcaata ccgtactccg ccagcagcgt caccatatcg
     3061 ctgtgatgaa cgccagcaaa atcaaaggcc agcaggctgg aatcctggca gcggaatgaa
     3121 cgaaagcctg gacgtttcgc cagcgcatct tccgccagcg ttgctaagct acggctccag
     3181 ttttcggcct ggttgatatc gtaatctgcc agccattcca gcgccgcgct taatcctatg
     3241 acaccagcca catttggcgt tcctgcttcc agtttccacg gcgcagattg agtcgtgaag
     3301 ccgtcaaaac tcacttcgtg aaccattttg ccgccgccca gccagggcga catcgcttcc
     3361 agcagttctg atttgccata cagcacgccg atacccgtcg ggccatacag tttgtgacca
     3421 gagaaagcat agaaatcgat atccagttgc tgaacatccg cggggaaatg caccgccccc
     3481 tgagcaccat caactatcac cactatcccg gctgaatgag caaaggtaat cgctcgcgcc
     3541 agatccgggc aaccgccagt tacgttcgac atctgaccca acgccagaat ccgactacgg
     3601 ggagtaatca gttccggcag caaatcgaca tctggcagtc gctgcgcatt aagcggcaat
     3661 ttgaccactt tggctccagt ttgttgggcg accatcagcc aggggacgag gttggcgtgg
     3721 tgttctgcca cgctgacaat aatctcatcg cccggttgca gacgcggacg cgcatagcat
     3781 tgtgccacca tgttgatgga ttcagtggtg ccgcgcgtcc agacgatagt tttatcatcc
     3841 ggtgcattca gtaattgcgc cactttctct cgtgcagctt cataacgcgc ggtcaggcgt
     3901 tgggcttcgg caaactggct gcgatggacg tttccggcgc tcagactgta aaactgtcgg
     3961 gtggcttcaa ccacggcttc aggtttaagc gcggtcgcgg cgctgtcgag atagacgccc
     4021 gcatcctgta gagcgggaaa ctgggcgcga aactgcgcgg gattaaaaac gttcatggta
     4081 ctcctcggct tgatgcaccg atcgtgacgc aaaattcact ttgatacaag gagagtgcta
     4141 accatcagaa ttatctggat atcctggcga agccgcgtca gtaacatatg ctgaatagtg
     4201 ttaggcgtta attttagcct gttaatcaat actattaaga tttattaatc gctttaagga
     4261 gaagatgatg aaaaagactg ccgcaattat ttctgcctgt atgctgactt ttgccctgag
     4321 cgcctgttcc ggttcgaact atgtgatgca caccaatgac ggacgtacca tcgtctctga
     4381 cggcaaacca cagactgata acgataccgg tatgatttcg tataaagacg ctaatggcaa
     4441 caaacagcag atcaaccgta ccgacgtgaa agagatggtc gaactggatc agtaattcgc
     4501 gatcgcaagg taaaaaaaag caccgcaatt aggcggtgct acattaatca ctatggacag
     4561 acagggtaaa tgtacaggaa gtgaaaaaag gtagctttgc taccatggtc tgaatcgcag
     4621 accaattgca aacacaacaa cacaacatca caaccgtaag ccaaaagttc accagaacac
     4681 gcattccgat aaaacttttc gttccggctc aggaagtgcc gccactatag gtatttgctg
     4741 gtagaagctc aacggacaat ttataatggc tcagattaaa aaaactaata ggttacacag
     4801 tgtgatctaa ttgttaaatt catttaacat caaagtttaa aagccatgtc taaacgatta
     4861 ccaccgctaa atgccttacg agtttttgat gccgcagcac gccatttaag tttcactcgc
     4921 gcagcagaag agctttttgt gacccaggcc gcagtaagtc atcaaatcaa gtctcttgag
     4981 gattttttgg ggctaaaact gttccgccgc cgtaatcgtt cactcctgct gaccgaggaa
     5041 gggcaaagct atttcctcga tatcaaagag atattttcgc aattaaccga agcgacgcgt
     5101 aaactccagg cccgtagcgc caagggggcg ttgacggtca gtttactccc cagtttcgcc
     5161 attcattggt tggttccgcg actttccagc tttaattcag cttatccggg aattgacgtt
     5221 cgaatccagg cggttgatcg tcaggaagat aagctggcgg atgatgttga tgtggcgata
     5281 ttttatggtc ggggcaactg gccggggcta cgggtggaaa aactgtacgc cgaatattta
     5341 ttgccggtgt gttcgccgct actgctgact ggcgaaaaac ccttgaagac cccggaagat
     5401 ctggctaaac atacgttatt acatgatgcg tcacgccgtg actggcagac atatactcgt
     5461 caattggggt taaatcatat caacgttcag caagggccaa tttttagcca tagcgccatg
     5521 gtgctgcaag cggctattca cgggcaggga gtggcgctgg caaataacgt gatggcgcaa
     5581 tctgaaattg aagccggacg tcttgtttgc ccgtttaatg atgttctggt cagtaaaaac
     5641 gctttttatc tggtttgtca tgacagccag gcagaactgg gtaaaatagc cgcctttcgc
     5701 caatggatcc tggcgaaagc cgctgctgaa caagaaaaat tccgctttcg ttatgaacaa
     5761 taatttacgt agggtacgac catgaccagc cgttttatgc tgattttcgc cgccattagc
     5821 ggcttcattt ttgtggcact gggcgctttt ggcgcgcatg tgttaagtaa aaccatgggg
     5881 gccgttgaga tgggctggat ccagaccggc ctcgaatacc aggcgtttca tacgctggcg
     5941 atcttaggtc tggcggtggc aatgcagcgt cgcatcagta tctggtttta ctggagtagc
     6001 gttttcctcg cgttaggcac ggtgctgttc agcggcagcc tttattgcct ggcactgtcc
     6061 catctgcgtt tgtgggcgtt tgtcactccg gttggcggcg tgagcttcct tgcgggctgg
     6121 gcgttaatgt tagttggtgc tatccgttta aagcgcaagg gcgtaagtca tgaataaggt
     6181 tgtattgctg tgccgtccgg gctttgaaaa agagtgcgcc gcagaaatta ccgataaagc
     6241 cggccagcgg gagattttcg gttttgcccg cgtgaaagag aatgcgggtt atgtcattta
     6301 tgaatgttat caacctgatg atggcgataa gttaatccgt gagctgccgt tcagttcatt
     6361 aatttttgcc cgccagtggt ttgtggtggg ggaactcttg cagcatttgc cgccagaaga
     6421 tcgtattacc cccattgtcg gcatgttgca gggcgtagta gagaagggcg gtgaactgcg
     6481 tgttgaagtt gccgatacca acgaaagcaa agagttgctg aaattctgcc gtaaattcac
     6541 cgtgccgcta cgcgctgcct tgcgcgatgc cggggtgctg gcgaactatg aaacgccgaa
     6601 gcgtccggtt gtgcatgtat tctttattgc accaggctgc tgctataccg gttactcata
     6661 cagcaacaat aattcgccgt tctatatggg cattccgcgc ctgaaatttc cggcagatgc
     6721 gccgagtcgt tccacgctca aactggaaga ggcatttcat gtgtttattc ctgcggatga
     6781 gtgggatgaa cgcctggcga acgggatgtg ggcggtggat ttaggcgctt gccctggcgg
     6841 ctggacctac caactggtga agcgcaacat gtgggtttat tccgtcgaca acggcccgat
     6901 ggcgcaaagt ctgatggata ccggacaggt gacgtggctg cgggaagacg gtttcaaatt
     6961 ccgtccgacg cgcagcaata tctcctggat ggtatgcgat atggttgaaa aaccggcgaa
     7021 agttgcggca ttgatggcgc agtggctggt taatggctgg tgtcgtgaaa ccattttcaa
     7081 cctcaaactg ccgatgaaaa aacgctacga agaagtgtca cacaatctgg cgtatattca
     7141 ggcacagctt gatgaacatg gcataaatgc tcagattcag gcgcggcagt tgtatcacga
     7201 tcgcgaagaa gtgacggtgc acgtccgccg tatctgggct gcggtgggtg gtcgtcgcga
     7261 cgagcgataa caggcaaggc cggggcgtcg tctccggcct gctacccttt ccgtcagcct
     7321 gttaccaaag aagttgcaac ctggcgttca tcagaaataa tgtgcgttac ctcatccaga
     7381 tgcccgatgc gggcttcccc tgaacgatta aatttacttt tatcaatcaa taacaacgat
     7441 tgcgcggcgc gttttaatag cattgatttg taatcagcgt tgatagcatt ggagtcccac
     7501 agtgcgccgc tgctatcgat cccttcacaa gaaaaaataa acagatcgat ttccagcgat
     7561 ttaagttggg aaatcagcga gggattgacg taacagccat atttgcgctc aagtgtgccg
     7621 ccggaactga tcagttgaat gcgttcgcgt ttaccgagtt catggcaaat cggatggcta
     7681 ttggtgaaga cctgaatgtt gatgtcaggc aactggcgtg ccagatacca gcaagttgaa
     7741 ctggcgtcta aggctatcac catcccttct tcaatccacg cgagcgcctc gcgcgcgata
     7801 tctgctttat gcgcataatg gcttttcagc ctgatgtgaa agggatcgcc actgtcttga
     7861 ttttgacggt ggatatattt agcgcgtcca tgattgcgca gaattttacc ctgcgtctgt
     7921 aattcattga gatcgcgacg aatggtttct ttactgacct ttagctgttc agagagagct
     7981 tccgtggtca ggctggtatg gttcagcagc aggtcgacta tcgcttgctg gcgtgccgct
     8041 ttcatatttt tcaccccatc attcgataac cgcccgcgtc atgcgggcgt accggtatga
     8101 gattacggtg ttaccccttt ttttaaaaga atattcccgt acttcgctcg ttcgcctgtg
     8161 ataacgatcg caaaggcttt ttgcgcccgt tcataaaacg caaaacgatt gatgcggatg
     8221 atgtcaggac acggggcttg tagtgaaagc gcattacggt aacgtcgttc tacttcagga
     8281 tcgagagtgt caccttctac cgctgccatc atcaccagcg gcggtgcata actgtccagc
     8341 tcaaataacg ggataatcgc ctggagcaag tcgctcacca acaggccatc agcgcggatc
     8401 acctgcggtc ccatcgaatg ggcgggaaag tgagcatcgg aaaaaataat ttcatctcca
     8461 tgtcccatct ctgccagcac tttcaatagt tcgggagaaa ttaacggcga aattgttttc
     8521 agcatttcac acttcctcta taaattcagg ttcagtttgc gggtagaaat aacggtactg
     8581 ataatgaatc tgtgcacggg cttcttccgg gctgttaaat tcccctacgc cataccaacc
     8641 gaacagcgca gctcctgcga cggtcgtttc ggcgtcgtcg agaactttta acggaatatc
     8701 aagcatattg gctttaatct gattccacaa tgtgttgcga cttcctccac cgactaacaa
     8761 taattcagag gccttaaagt gcccgatttt ttccagcatc tgtagattgc gctgtaattg
     8821 cgcagttaac ccttccagcg ccgcgcgata gaaatgcccc cgcgtggtat taagcgtcac
     8881 tccttgccag ccagcgttct gacacgacaa taaatcacac tgcattttca cgccatccgc
     8941 gccaggcgcg atcagacgag cctcttcaat caacatttgc cagggtgttt cagccgtcca
     9001 gaacagtttt ctcacccatt ccagcacacc ggatgccagc cattgcatac ctgggttata
     9061 caaccctgcc tggctatcca gttcgcaggt ggaaccggcg tactgactta acagcgaagt
     9121 atcaacctgg gcgctgcgaa ccattaaaat ttcccatgta ccggaagaga gcacgggttc
     9181 attttgttca gcaccagcgc caaaaagggc gaactgggta tcgtgacctg cggaaatcac
     9241 cggtatgcca acgggtaagc cgagcattgc tgcggcgctg ttctgtagcg taccaatctg
     9301 ttcacccgct tccaccagac gagggaagag tcggcgtgga ataccggtgg cttgtaaaat
     9361 ttgcggactg aaatcgcgtt gctggatatc cagcatctgg ctggttccgg ccatcgtgat
     9421 atcagtagtg aattcgccgg ttaaacggtg gttaatcagc gacgaaataa agagccaggc
     9481 gtgcgcgcgt tccagcagtt gtggatgatt ttctttcaac cacaccaact tatataacgt
     9541 attgaaacta aaggctccga cgccagaaat agcctgcaac cgctgtgcgg agattaaccg
     9601 ttcaatattg tccataaccg ctgctgttcg cggacatttc cagctaatga tcggatagag
     9661 cagattgcct tgcttatcta ccagagcgcc atccacacca aaggtggtga cggcgatacc
     9721 gcggatgtgg cattcagtca gttcactatt gatttgccga cagcaatcag caaagcgttg
     9781 caaaatggcg tctaaagacc actggtgcca ggtgttgttt tccatcgcga tatcgctggc
     9841 attaggcgtt gaggcgcggg caacaatttt tccctgccga ttaaccgcga tggccctgac
     9901 attggtcgcg ccacagtcga gtaccaggat cacttcttgt ttcatgatcg ctcctgcaat
     9961 atagccggat aacattgctt atccggctaa ccactcttgc ttgttcaggc ccggcagccg
    10021 gagcgaccgg gcatcacatc agggagtaat gtattaacgc ttgtacaacg gaccgtagtt
    10081 ctggcaagcg cggtaatcct ggccttcaat atccatgccg tgcgcagccc aggcagaagg
    10141 acgatacact ttggtctctt caacgttgtg catacatacc gggatacgca gcatggaggc
    10201 gagagtgata aagtctgcgc caacgtggcc gatggtcaga accccatggt tagcgcccca
    10261 gttcgccatt accgagtaca catccgtaaa cggcccttta ccggtgaggc gcggtgcaaa
    10321 ccaggtggtt ggccaggttg agttggtgcg tttgttgagg atgtcatgca catccttcgg
    10381 caattccacg ctccagcctt ccgcgatttg cagtaccggt cccaggcctt tgatgatgtt
    10441 gacacgagtc atggtgaacg ggacgccgcc ttcggtaagg aagcgggaag agtaaccgcc
    10501 gccacggaag tattcgtgga tcgccgggca ccattcggta gcggcgaggc aagcgtcagc
    10561 ctcttgctga gagatttccc agtgcggctt catcgtcggg ttaccttcgc tgtcgcgttg
    10621 tttacaggaa ccgtccagcg cagcagaacc ggagttgatc aaatggatga tgccgtgttc
    10681 tgccagtcca tccagtttat gccccgttac acgctcaatt gcttctggtg accagtaggt
    10741 acgcacatcg gcaaatacct gagcggtgcc ggtgagctgg tgacccatta gcattgccac
    10801 gccgttaaga ctgtcgtttt cggtcgccac gacaaagggt tcgcgcacgc cattccagtc
    10861 aaatgaactg ttgaggatcg cttcggcggt gtcaccattg ggatattgat cggtccagtg
    10921 acgttgcccc tggaagcccg cagcgatggc gttgtagcca agtgattctt ccacgcgacc
    10981 aatatcggcc agtttgctgt tgccttgcat catgtcgcgg atacacatcg ccatcagtaa
    11041 actttcgcgc agaactgcgc ggctttgctc ggcattgcgt tgatactgtt tgttattttc
    11101 atcttcgcca tagcggaagt ttttatcagc ccaggccagt gccatttcca gttcggcttc
    11161 gtcgtaaatc ttctgatcga tacggcgacg cagttcggtc atatccaccg cctggacttt
    11221 cattcccagc caggattcaa agaagttgtg atcaacaata gaaccggcga tacccatcga
    11281 aacgccgccc agcgacagat agcttttacc tttcatgctg gcgacggcca aaccggcgcg
    11341 ggcaaagcgc agcagttttt cttcaacatc ggcaggaatc gatgtgtcat cggcatcctg
    11401 aacgtcatga ccgtaaatgg agaatgccgg gatgcctttc tggctgtgag ctgccagagc
    11461 cgctgccagg taaacagcgc cgggacgttc agtgccgtta aagccccaaa tggctttcgg
    11521 gcgggttgga tccatgtcga tggtttcact gccatagcac cagcaaggcg ttaccgtaat
    11581 ggtgaggcct acattctggc tgctgaattt ttcttcgcaa gcagcggctt cagccatacc
    11641 cgcgatacag gtatcggaaa tgacacactc gacggcagct ccgcaggcat ggcgcagttt
    11701 ctcggtcagc agtgcggccg tagctttcgc catattcatt gtttgttctt caagcgactc
    11761 acgaacaccc atgcgacgac cgtcaataac cgggcggata ccaattttcg gtaagctgat
    11821 tttgttcatt acagattcct cactttattc ggaaaatatg ttcagttagt tgccgtttga
    11881 gaacggaaac gggcaaagat aaagatgacc gcgaagcaga gtgcggggat cagttcagca
    11941 gtggggatgt tgcccgccgc gtcactgaca aaacccatga ccggagtgac aataccgccg
    12001 ccaatgatgg tcataacgat gaaggacgaa ccgtatttgg tgtcctggcc gagattctta
    12061 atgcccagcg agaagattgt tgggtactga atcgacataa aggcgctgca taaagtcagg
    12121 gctattaagc ccacatgact gccagcgaag gctgagatca ggcacagtgc catagcgatt
    12181 aatgcgtagg cggccaggac tttgtgtggt gcgaagcgac tgatgagcca ggtaccggtg
    12241 aaacgaccaa taaagaagca caccatggtt ccggttaaat agttagcggc aaagcctgca
    12301 gtcatgcctg gaatttcttc tacagcgtag cgaatcaaat agctccagca ggccgtttgt
    12361 gcgccaacgt agcagaattg cgctaatact gcccagcgcc agtggcgaat acgcgccagg
    12421 cgagaaagcg atgcggagaa cgatccttgt ttggcgtcac tgtgattatc actctgcaat
    12481 gccgggaatt tcgtcagcat gatcagcagg gcgaccagta acacgatagc cacgatgatc
    12541 atataaggtg tctgtaccga taataccagg ctgtgtttat acgcactcaa ttgctctgga
    12601 gacattttat cgagaacgtc ttgcgattga tgtggcacgt tagacaaaat aagactttgc
    12661 ccaaagacaa ccgcgataat tgcgccaaat gagttaaatg tttgcgcaag atttaagcgg
    12721 aagtgaccac tgctttccgg ccctaatacc gtaacaaaag ggtttgcggc agtttccaga
    12781 caacctaatc cggctgcaat aataaatagg ccaactaaaa ataaggtgta gttcattatt
    12841 tctgcggcgg gccagaataa tgcagcaccc aaggcatata aaaa
//
LOCUS       DBFIFI010000121        12816 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.121, whole genome shotgun sequence.
ACCESSION   DBFIFI010000121 DBFIFI010000000
VERSION     DBFIFI010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12816
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.121"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..829)
                     /gene="yhaJ"
                     /locus_tag="N7B39_003214"
     CDS             complement(<1..829)
                     /gene="yhaJ"
                     /locus_tag="N7B39_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HGU2423147.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     "
     gene            934..1635
                     /gene="yhaK"
                     /locus_tag="N7B39_003215"
     CDS             934..1635
                     /gene="yhaK"
                     /locus_tag="N7B39_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HGU2423148.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQHLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRTYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            1658..1822
                     /gene="yhaL"
                     /locus_tag="N7B39_003216"
     CDS             1658..1822
                     /gene="yhaL"
                     /locus_tag="N7B39_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HGU2423149.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(1956..3266)
                     /gene="cyuA"
                     /locus_tag="N7B39_003217"
     CDS             complement(1956..3266)
                     /gene="cyuA"
                     /locus_tag="N7B39_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HGU2423150.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHDG
                     VVFTQQACVAEGEQESPLSVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3294..4625)
                     /gene="cyuP"
                     /locus_tag="N7B39_003218"
     CDS             complement(3294..4625)
                     /gene="cyuP"
                     /locus_tag="N7B39_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HGU2423151.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFIIVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGLVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(4900..6264)
                     /gene="tdcG"
                     /locus_tag="N7B39_003219"
     CDS             complement(4900..6264)
                     /gene="tdcG"
                     /locus_tag="N7B39_003219"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HGU2423152.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKAQEELLSKTYYSVGGGFIVEEEHFGLS
                     HDVETPVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6336..6725)
                     /gene="tdcF"
                     /locus_tag="N7B39_003220"
     CDS             complement(6336..6725)
                     /gene="tdcF"
                     /locus_tag="N7B39_003220"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HGU2423153.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(6739..9033)
                     /gene="tdcE"
                     /locus_tag="N7B39_003221"
     CDS             complement(6739..9033)
                     /gene="tdcE"
                     /locus_tag="N7B39_003221"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HGU2423154.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDKNGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9067..10275)
                     /gene="tdcD"
                     /locus_tag="N7B39_003222"
     CDS             complement(9067..10275)
                     /gene="tdcD"
                     /locus_tag="N7B39_003222"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HGU2423155.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSLRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10301..11632)
                     /gene="tdcC"
                     /locus_tag="N7B39_003223"
     CDS             complement(10301..11632)
                     /gene="tdcC"
                     /locus_tag="N7B39_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HGU2423156.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLVLKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(11654..12643)
                     /gene="tdcB"
                     /locus_tag="N7B39_003224"
     CDS             complement(11654..12643)
                     /gene="tdcB"
                     /locus_tag="N7B39_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HGU2423157.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(12742..>12816)
                     /locus_tag="N7B39_003225"
     CDS             complement(12742..>12816)
                     /locus_tag="N7B39_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2423158.1"
                     /translation="VLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         3278 a         3505 c         3060 g         2973 t
ORIGIN      
        1 gacgccaggc cataataata tcgatctcgc tggtcgattc cgggctgacg acacgcaacc
       61 gcccttccgc aatatctttt tcgaccatcg gatacggcat cgtcgccacg ccaagccccg
      121 ccagtaatgc ctgacgttta tcttcaatcg tgctcaccgt taagcgcggc tgtttgtcca
      181 gcagctgtac agtcaacacc gggcgctcac gagcggtatc cgccaccgca attccacgat
      241 atttcacacg cgtcacttca gataacggtt ccggctcctg atgaatcggg tgatcaggcg
      301 cggcaacgta gacgttcatt aacgtataga gcttgcgcga gttgatctcc gacgaggaac
      361 gaaaatgcat atccggcgcg ataacaatat ccgcccgccc ctgctccagc cgttcccacg
      421 cccccgccag cacttctgtg atgattgcca gttgggtatt ggcttttgcc gccagtttgt
      481 cgattaacgg gaaaaaggca ggtgtcggta ccagcgcttc ggtcacaatg gtgagatgcg
      541 tttcccaacc gcgcgcgaga gcttccgcat cagtagtcag tttatctgcg gcttccagca
      601 aaacgcgccc tcgctccaac aacatcctcc cgacattggt gaatttggtg cgatggcccg
      661 agcggtcaaa cagcaccaca tccagctctt cttccagttt ttgcatggtg tagctaagtg
      721 cggaaggcac gcgtcccagc tcatccgctg ccgccgcaaa actgccccgg cgatcgatcg
      781 catccataac ccgtagtgct tccagcgtta atgccctttc tttggccatt tcgttctcat
      841 tcaggaaatt tgaacatacc gggcagaata tctggctaac aatgtagcgt ccagcccctt
      901 accataaaag gaagtaaaga gaggtcaaga attatgatta ctacccgaac tgccaggcag
      961 tgtggacaag cagactacgg atggttgcag gcccggtata ctttttcctt tggacactac
     1021 ttcgacccga aattgttagg ctatgcctcc ctgcgtgtgc ttaaccagga agtgctggcc
     1081 ccaggtgccg cctttcagcc gcgaacctat cccaaagtcg atattttaaa tgtgattctg
     1141 gatggagaag cagagtatcg cgacagcgaa ggcaatcatg ttcaggccag cgccggtgag
     1201 gcgttgctgc tctctaccca gccgggtgtc agctacagcg aacacaatct cagcaaagac
     1261 aaaccgttaa cacgaatgca gctttggctg gacgcctgcc cgcagcgaga gaatccgctg
     1321 attcaaaaac tggcgcttaa tatgggcaag cagcatttaa tcgcctcgcc agagggtgcg
     1381 atgggaagcc tgcaattacg ccagcaagtg tggctgcacc atatcgtgct cgacaaaggc
     1441 gaaagtgcga atttccagtt gcatgggcca cgcacgtatt tgcaatcgat tcacgggaaa
     1501 tttcatgcgc ttacgcatca tgaagagaaa gcggcgctga cctgcggtga tggggcgttt
     1561 attcgtgacg aggctaacat tacgctggtt gccgattccc cactgcgcgc tttgctgata
     1621 gatttgcctg tctagttgtt tttacaggga gatgatgatg agtaaaaaat cggccaaaaa
     1681 gcgccagcca gtgaagcccg tggtggcgaa agaacctgct cgcaccgcca aaaattttgg
     1741 ctatgaagag atgttgagcg agctggaagc catcgtcgcg gatgctgaaa cgcgtttagc
     1801 cgaggatgaa gctaccgcgt aaagacaggt tgccggatgc ggcgaaaacg ccttatccgg
     1861 cctacaaaat ccaacaacat taacgaatta aacaacccgt aggccggata agatgcgcca
     1921 gcatcgcatc cggcacgata cccaaaacct ggcgtttatc tggccttgct cgccataatc
     1981 tcgataatct gccgatccgt ttgctgcatc gaatggcttg ctaacgcaca caggttggca
     2041 atcgactgct caacatcatg cgccacgatc ccttcattgc cggtcacggc ggtatcatcc
     2101 agcgccatta acaccgcttt ccacgcagcc gaagcactgg tcgaaacctt catcgcgcag
     2161 ctgttcgacg caccatcgca aatcatgccg ctgacatcgc cgatcatact gctgatcgcc
     2221 atcgagatgg tttcataacg cccatccacc agccatgcca tcccggcggc ggcccccatt
     2281 gctgcggtcg ttgcggcaca cagcgcagac aaacgcggta actggttatg gatgtaaatt
     2341 gcgctcaaat gagaaagcat cagcgcacgc gccagccgtt catcatccgc tccgaagtgt
     2401 tctgctacca ccaccacggg cattgttgcg gtgatcccct ggttacccga gccggagtta
     2461 ctcattgccg gaagcgtggc gccgcccata cgcgcatcgg atgccgcgct ggtacgaatc
     2521 acaatggatg aagagagatc tttcgccagc aaaccgcgct cgcactgttt ttccagcgtc
     2581 gcgccaatat gcagccccca cttaccgctc aaaccttcct gcgataacgc acaatttagc
     2641 ttcgcggaat cgagaataaa gcggatcgcc gcaaacggga cttcattgac gaacttcagg
     2701 atctcagcca gcgtcgttct ggaaagcacc gatagcggag actcttgctc gccctctgcc
     2761 acacacgcct gctgggtaaa caccacacca tcgtgcgtct caatatgcac aatgttggta
     2821 tgcccgccga cgatagtgac acacgcccac ttctcaccgt tccagacttt ggcgcgtgag
     2881 aagaggattt cattgcaagg ttcctggatc ttaacggaga ctttccccgc cgccagcagt
     2941 gctttggcat cggcaattgc ctgcgcagtt gcgtctttca gcacttccag cccggcgttg
     3001 gcatttccac ctaacgcccc cagcgccgcc gcaatcggca gccccaccat tcccgtgccg
     3061 ggaacggtga cgcccagacc gttcttcatc agatttggcg aaacccaggc ttctacacgt
     3121 tcaaccggac cttccagttc tgccgcagca accgccgccg ccagcgccag tgaaatcggt
     3181 tcagtacatc ccagcgccgg ttttacttcc tcctgaacgg cgaggatgta acgctgccat
     3241 aacggattta aagtcgaatc aaacatatta aaaaccttaa aatttcaggt aaatcaagaa
     3301 aatgccagga acggagaaac acaaagcaac aaaccagtga cgataatcag gtacagagac
     3361 atccctttgt atttgtgcaa tgccggtact ttgtaaacca gccacgccgg gatcaggcac
     3421 cctaccaagc cgaaaatcgg gctacagata gaggtgaagc tcaacaccgg tgcgttcagt
     3481 acgatggcgc tccaggccag caaaatggcg aaaatcatga tgccgcgctg aacaagattt
     3541 tcgttaatct tctcggcagg catcttgcga cgcaggatgt tcattacgat cccttgcgtt
     3601 gcttcgcgaa aacctaaata gacgccaaag aatgcagtca tgacagcaaa gatattgaga
     3661 atgacgctga ccactttcac ccacgctgcg ccgtcaccgc taataaactg tgcggcaatc
     3721 gccagcgcgg aaatattctg ctcgtaggct tttaccgctt cgtcatgtcc catcgccagc
     3781 gtgaacgaca cggcgtagaa aaagacgatg ataaacaaaa tgccaaacgc gatattcatt
     3841 gcccgcaatg ctttatgccg cgctacttca atcgattttt cccgcgagcg ataagagatc
     3901 accatcgggc ttaacgtctg gataaacaga atcgacgtca gggtaaacgg cagcgtaata
     3961 atagcgtttt tcaccagcag ccccagcggc ggtagcgaac cgacgttata cagatgccac
     4021 attcccacca tcgacacgcc cagcgccgcg accaccagca gcttggtcag caccatgcca
     4081 gtcgaaattt tgaataacaa tttctcgccg cgtgaagaga tcgccaccag aatgcaaatc
     4141 agtaccagac cataaaaggg actgtctgac agcaaccctt ccgtcacgcc gaaggtatgc
     4201 aggtaggaag cactatcgtt ggtgatggcg gtggaataga cgaacatcca aatcaccagc
     4261 attacgaaat agagtgcacc taacaggatg ccccagtttt tacctaaata accgctaatg
     4321 acgctcgggt aatctttaca ttctggtgat tctgccagcg tattaataaa caaccgctga
     4381 aacagataca ttgccgggta accaatcacc gatgagagca aaaataccca caatcccatc
     4441 aaaccgacct gcaccgggag aaaaacaatc cccgcgccaa tcgccatccc gatactcata
     4501 atcacccagc cggtgtcagt actgtcgaat ttgatcgctt ctcgccactc gctctcactc
     4561 attccagcac gacccgccgg ggtcgaagcg tctgcaatga cgcctttatt cgatgcaatt
     4621 tccataattt ctcgctcaat attttgtagg gcttattatt tttttccgag ccgcatcaag
     4681 gcgatatgcg gtctacgttt ttgcaggcga gttattagaa taaagaaatg atacgcgcga
     4741 ggtcagagaa aatcttcaca atcaagactt tgaaataacg gagatggata agaattttct
     4801 actaaattaa tcgcaagaga atattcactc tctatttgcg gcagatcaca aaaaaaaggt
     4861 gcacatttgt gcacccaagg atgaaagctg acagcaatgt cagccgcaga ccactttaat
     4921 ggccagtccg ccgcgtgatg tttcgcggta tttatcgttc atatctttgc cggtttcata
     4981 catcgtctcg atcactttat cgagtgaaac acgcggtgcc gaggtgcggc gcatcgccat
     5041 ccgcgcggcg tttactgctt tcacggcatt aatggcatta cgttcaatgc acgggatttg
     5101 tacctgtccg gcaaccggat cgcaggtcag tccaaggtta tgctccatcg cgatttccgc
     5161 cgcattgcat acctgcgccg gactgccgcc cagtagttca gttaaccctg ccgccgccat
     5221 tgaacaggcc acgccaatct ccccctgaca gccgacctcc gcgccagaga tggaggcgtt
     5281 cattttatac agcgcgccaa tagccccggc tgcaagaaaa tagcgggcaa ttgaccgctc
     5341 gtttaccgga cgacggaact tatcgtaata agccagcact gccggaataa tgccgcacgc
     5401 accgttagtc ggtgccgtta ccacgcgccc gccagctgcg ttttcttcac taaccgccag
     5461 cgcgtacatg ttgatccagt cgatgacatt catcggatcg ttagagatgt tatcgctgga
     5521 aaccagctga cgacgcagcg ctacggcacg gcgcggcaca ttgagcggac caggcagcac
     5581 gccttcagtg ttcatcccgc gttcaatacc gtcatgcatc acttgccaga tacgggcaaa
     5641 accggcgtca atttccgctt tactgcgcag cgctagctcg ttgtgcatca tcaggccaga
     5701 aatagacagg ccgttgtaat cacacatttt cagcagttca cctgctgagt ggaaatcgta
     5761 aggtacgggt gtttcgacat cgtgcgacag gccgaagtgt tcttcttcga caataaaccc
     5821 gccgccgaca gagtaatagg ttttacttaa tagctcttcc tgtgccttcc aggcagtgat
     5881 ccgcattccg ttctcatggc gaggcaacat ttcgggatgg aagataatgt tctttgctac
     5941 aggaaaatca acaatatgcg caccagatgc cactggcagc cgcccgctgc gcgttactaa
     6001 ctctataaat gcagggatct catcaatgac aacatcctgc ggactgtttc ctgccagtcc
     6061 catgatgatg gcgacatccg tggcatggcc tttgcccgtc agtgacaacg acccgtacag
     6121 atcgaccaca atatggctcg tcgcggttaa taagccgcta ctttccagcc gatcaataaa
     6181 actttttccg gcattcattg gccccacggt atgcgaactg gagggaccaa tcccaatttt
     6241 gaaaatatcg aatgcactaa tcatatccac accctcggat tgccgttcag tgaagtggag
     6301 cggaacgacc ttacgaccgt cccgctcacg aggctttacg cactacgtac tgcgatagct
     6361 tcaatttcca gcttcacatc tttcggcaaa cgcgcgacct gtacacagct ccgggtcggg
     6421 taggtcgcct gatgctcatc gaagaattgc ttatagactt cgttgatggt ggcaaaatca
     6481 ttcagatcgg tgatgaacac ggtcatcttg atgatatcgc ccacgctcag cccggcagca
     6541 accacgatcg ctttgacgtt ttcgaggctt aaacgcgcct gatcttgcac atcagccggg
     6601 atctcaccgg tctgtgggca aaccggaatt tgaccggagg tgaagaccat gctgcctaaa
     6661 tcaacgcctt gaacataagg gccgattgcg cctggggcac gttgcgtttc gataatcttt
     6721 ttcatacatc ctccggcgtc agagcgcctg ggtaaaggta cgtgaaataa catcctgctg
     6781 ctgttcacgg gtcagtgcgt tgaagcgcac ggcgtagcca gagacacgga ttgtcaggtt
     6841 aggatatttt tccgggtgct cgatggcatc cagcagcatt tcccgattca ttacgttgac
     6901 gttgaggtgt tgaccgcctt cgacatccgc ttcgtggtgg aaatacccat ccagcaggcc
     6961 gacaaggttg gttttacgta ctggatcttc tttgcccagc gccgcaggga cgattgagaa
     7021 ggtgtacgag atcccatctt tggcgtaggt gaacggcagt ttcgccaccg acgtcaatga
     7081 ggccacggca cctttgcggt cacgaccatg catcgggtta gcgcccggcg cgaacggtgt
     7141 tccggcgcga cgaccgtccg gcgtgttacc ggttttctgg ccgtacacca cgttagaagt
     7201 gatagtcaga atcgactggg tagggacggc gttgcgatag gttggcagcg ctttaatttt
     7261 cttcataaaa cgttcaacca ggtcgcaggc aatgctgtct acgcgctcgt cgttgttgcc
     7321 gtactgcgga tattcaccgt cgatttcaaa gtccaccgcc aggccgtttt tgtcacggat
     7381 tggtttcacg cgggcatatt tgatcgcaga cagggagtcc gttgccaccg acaggcccgc
     7441 gatgccgcac gccatggtgc gatagacatc acgatcgtgc agcgccatca gcgaagcttc
     7501 gtagctgtac ttgtcgtgca tgtagtgaat gatattcagc gcgctgatgt actgcaccgc
     7561 cagccagtcc atgaagtgat cgaggctgtc catcactttg tcgtaatcca gcacatcgtc
     7621 catcaacggt gccgttttcg gcccgacctg aatcttcagc ttctcgtcca ccccgccgtt
     7681 aattgcgtac agcagtgttt tcgccaggtt agcgcgtgca ccaaagaact gcatttgctt
     7741 accaatcacc attgggctga cgcagcaggc aatcgcgtaa tcgtcgctgt tgaagtcagt
     7801 acgcatcaga tcgtcgtttt catactgcaa ggaagaggtg acgatcgaca cctgcgcggc
     7861 atattttttg aaggcaatcg gtaattcttc cgaccaaaga atggtcaggt taggttccgg
     7921 tgcaggcccc atagtgtgca gggtgtgcaa atagcggaag gagtttttgg tcaccagcgt
     7981 acgaccgtcc agccccatcc cgccgatcac ttccgtcgcc cagattgggt cgccggagaa
     8041 cagcgaatca aattccggcg tacgcaagaa gcgcaccata cggatcttca tgatgaagtg
     8101 atcgatcagt tcctgtgcct gctgctcatt gagtacgcca gctttaaagt cgcgctcaat
     8161 gtagatatcc aggaacgatg ccgtgcggcc cagcgacatc gcgccgccgt tctgtgattt
     8221 cactgccgcc agataagcga agtagagcca ctgcaccgct tcctgcgcat tctgcgccgg
     8281 gcgagagata tcaaagccat atttcgccgc catttcctga atctgcaaca gcgcatgacg
     8341 atgctctgcc agctcctcac gcagacggat ggtggcttcc agatcctcgc ctttttccag
     8401 acgagactgg agatcggcaa attgcagttc gcgttcacgc accagataac tgatgccata
     8461 cagcgctacg cggcgatagt caccgataat gcgcccacgg ccatagccat ctggtaaacc
     8521 ggtcagcacg ccagatttac ggcagcgcag catatccggt gagtaaacat caaatacgcc
     8581 ctggttatgg gttttacgca gatcggtaaa cagatattca aattcactgt ccatttcacg
     8641 accataggcg tggaatgaac ttttaatcat attaatgcca ccgaacgggt gtaacgcacg
     8701 tttcaacggc gcatccgttt gcaggccaac aattttttcc agcggctggt taatatatcc
     8761 cgcatcatga gcggtaattg tggtggcaat attggtatcg aaatcaaccg gcgcgtgggt
     8821 tgcgttttca atacgaatgc cttccatcac cttttcccac aattccgtgg tggctggcgt
     8881 cgcttcagcg aggaaagatt catcgccttc atacggtgta tagttatgtt gaataaaatc
     8941 gcggacatta atttcgtttt tccagtccgt acctttaaag ccaagccatg cgtcggcgta
     9001 cagcttatcg ctggtatcaa tatctacctt catgaaaaat aatctctcta caatacttca
     9061 actaaattat gcaaattctg cgggtgcgtt aactttgcct aaatgaatgg catccaaagc
     9121 aatcattttt tcttcgttgg tcggaattac ggcgcaaatg acacgcgcat tttcactgga
     9181 aacaattcgc tcgccaaaag agttagatcg attattcatt tctgtatcaa tctctacgcc
     9241 taatacagcc aaatgttcca tgaccagacg acgaattaaa cttgaattct ctcctattcc
     9301 gccggtgaat ataattccat ccaggcgacg taatgaagct gcgtgtccgg caatatgacg
     9361 ggcaattcgg tgaacaaagg ttttaattgc cagctgcgcg cgttcgtgac cttcatgcca
     9421 ggctttttcc agaacacgta aatccgaaga aagaccggaa atacctaata atcccgactc
     9481 tttattcact acgcgttcca ggtcacccag gctctggttg gtttggctgg cgacccagga
     9541 catcgcacca aagtcgacat cgccactgcg ggtacccatc atcaagcctt ccagcggcgt
     9601 cattcccatt gaggtgtcaa cactctgacc gttgcgaact gcgcagattg acgcgccatt
     9661 gccaagatgc gccacaacca ggccggagtc atcttccgcc agattcagca aagaatgtgc
     9721 gcgcagggaa acatagcggt gcgacgtgcc gtggaaacca taacggcgta cacctaactc
     9781 ttcataatat ttccacggca ggccgtataa ataagcttcc ggagccatcg tctggtggaa
     9841 actggtatca aataccgcca cctgagttac gcccggaaat aattgctgcg ccgattcaat
     9901 accacttaaa ttggcgtaat tatgcagggg tgccagtgga gaaacgcgac ggatattatc
     9961 aatgacttca tcggtaataa tggcggactc ggtaaaaata ctgccgccgt gagcgatgcg
    10021 gtggccaatt aaggccacgc tgtcatttaa attccgtttt tccagttcaa atgcaattgc
    10081 cttcaatgca ccttcgtagc tgtggtgagc cagcggtgct ggctctcccc catttacgga
    10141 taagaatgca ttttccgagt taataccgtc ggcaatacct gacattaata cttcacagtc
    10201 gctggcatcg agtacggaaa acttaatcga agacgaacca cagttaataa ccaaaacaac
    10261 cggaaattca ttcatctctt ttctcatcct gagttacgga ttaaaacagt ttatatacga
    10321 tgttcaggat ggtcagcaga ccaatcacgg taacaaacac gttatccaga cgaccacggt
    10381 atttcgccag agacggcgct ttacggatgg catacatcgg caacaggcac agcagggatg
    10441 cgataatcgg tgcgcccatg gcttcaatca ggtcgaggat gttcgggttg gcgtaggcaa
    10501 caacccaggt ggagcccatg atgaagatca tgctgatagt gttcagttta cccagcgata
    10561 ctttagtttt gtcgccttta taaccaaact tcaggaccag gccattcaga ccttccagcg
    10621 ttcccagata gtgaccgaag aaagatttga agatagccac gagtgcgatg atggaagccg
    10681 catattccag tgtaatcgcg aacgttgttt tggtaccggt catggacgca aagtggttag
    10741 ccagataaga aagcactgga atattctgcg ctttggcttc cgccatgttg gccggagaca
    10801 gcgtaaacag gcagctaaag gcaaagaaca tcaccactgc aaccatcagc atgctggcac
    10861 gagaaatgat ttgggaacat ttacgttcgg tgaagtcgcg accgaagtct ttctcatact
    10921 cttcacgttt agaaaccacg aaggaagaga cgattggcga gaagttaaag gagaaaacca
    10981 tgatggaaat ccccagccag acagtgatca ggataccgtc atgaccggtt aacgacagcg
    11041 aaccgaggtc aacctggtcg ataactgcag agttccagta agggatcagc gacaaagaaa
    11101 tcagcaccag gctggcgata aacggccata ccaggtagct catcacttta accatcagat
    11161 ccttaccaaa ccagatgacg aaagccatca gcagcaacag gaacagcgcc acaaagccgc
    11221 gattcagcgg tgcaaagccg agctggtttt cccagaacgt cataaaggta ttggtaatag
    11281 taacgccata aatccacagc agtgggcaaa tcgcgaagaa gtacaggaac gtgataacca
    11341 cgccgccagt tttaccaaaa tgctcttcca ccgtttccgt aatgttgccg gaagggttag
    11401 agccagaaag acacagacgc gccagcgccc ggtggcaata aaatgcgatg gggtatgcca
    11461 ataccaacat cagaagaatc gggatcagtc cgccaaaacc tgcgcggata gggaagaaca
    11521 gcaccccggc gccgattgcc gtaccaaaca agcctaacgt ccatgtggta tctgatttac
    11581 gccaggacga ttgttttgtc tggctggata caatgctatc tgaagtactc atatcctatc
    11641 ctcaacgaat taattaagcg tcaacgaaac cggtgatttg agagacgcga gaaagatcga
    11701 tattgccgcc ggaaataata ctgacggttt ttctgttttg aatatattgg tctaatttac
    11761 cgcttaataa tgcagcacat gccagagcgc ctgcgccttc ggtgacgact ttattgcgct
    11821 gaattaaggc aatcatactg tttctgattt cgtcttcgct gaccagcacg atgtcatcga
    11881 ctaattcacg aacgatttcg taagttaaat tacccgggcg ggagacatca caaccatccg
    11941 ccagggtgcc ggtagttcgg tgcgtggtta tttctccgga gtggaaagaa gccgccatgc
    12001 cgtgaacgtt ttcagactgt acgccaataa cacgaatggt cgggttaata gatttaattg
    12061 ccaccgcaat accagcaatt aaaccgccac caccaattgg cacaatcacg ttatcgacat
    12121 catagagatc ttccataatt tccaggccaa tcgttccctg accagcaatc actttcggat
    12181 catcgtaagg tgggataaaa atacggcctt ccatttcgac aatttcgctc actttagcga
    12241 tagtgtcgtt gaagttatca ccatgcagaa cgacttctgc ggagtagtcg cacgttgccg
    12301 ctactttgga tttcggcgcg ccttttggca tcaccacttt accgtcgata cccagcatcg
    12361 cgcaggagag cgaaacccct tgcgcatggt tgcccgcaga acaggccacc acgcctttgc
    12421 gtttttccgc atcagtcagt gaacttaatt tattaaatgc gccacgaatt ttaaatgaac
    12481 ccgtacgctg catattttca aacttcagga atatttcacc tttgcaacgt tcactaaaat
    12541 agttggagcg aggcatgcct gttttataaa ttcgcccagc cagtcgttgt ttcgcttcaa
    12601 taatgtcatc aatagcaacc ggcagatcgt atgtaatatg cattataaaa cctcttcgcc
    12661 tgaattaaat atgtaggtaa ccgtaaccga cacctgcaag acagacaggt ggattattta
    12721 tgtttaaata aaaacaaata actaaccaac ttctattaat tgccttcgtc tacacccatt
    12781 ataagatgaa tactctttgg ctaattccac caaaac
//
LOCUS       DBFIFI010000122        12708 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.122, whole genome shotgun sequence.
ACCESSION   DBFIFI010000122 DBFIFI010000000
VERSION     DBFIFI010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12708
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.122"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..820)
                     /locus_tag="N7B39_003226"
     CDS             complement(<1..820)
                     /locus_tag="N7B39_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000217053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU2423159.1"
                     /translation="MTVKYYAILTNQGAARLANATMLGSKLNLTQMAVGDANGVLPTP
                     DPAQTKLINQKRIAPLNLLSVDPNNQSQIIAEQIIPENEGGFWIREIGLYDDEGVLIA
                     VANCPETYKPQLQEGSGRTQTIRMILVVTNTEAITLKIDPSVVLATRKYVDDKVLELR
                     LYVDDQMRNHIAAQDPHTQYAQKHNPTFTGEPKAPTPAAGNNTTRIATTEFVQTAITA
                     LINGAPDTLDTLKEIAAAINNDPKFSTTINNALSGKQPLDETLTHLSGKDVAGLL"
     gene            complement(817..1428)
                     /locus_tag="N7B39_003227"
     CDS             complement(817..1428)
                     /locus_tag="N7B39_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HGU2423160.1"
                     /translation="MSDSRLLPTGSSPLEVAAARACAEIEKTPVSIRELWNPDTCPAN
                     LLPWLAWSFSVDRWDDKWPEATKRAVIRDAYFIHCHKGTIGAIRRVVEPLGYLIEVRE
                     WWQLNEEPGTFRIVVGVLEQGITEEMYQELERLVADAKPASRHLTGLAISLSTTGNIF
                     AGAGCYHGDALTVYPYTPEAIIVGGDYFPASAIHLIDNLRVNA"
     gene            complement(1421..2329)
                     /locus_tag="N7B39_003228"
     CDS             complement(1421..2329)
                     /locus_tag="N7B39_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HGU2423161.1"
                     /translation="MPIIDLNQLPAPDVVEELDFETILAERKATLISLYPEDQQEAVA
                     RTLTLESEPLVKLLEENAYRELIWRQRVNEAARAVMLACAAGNDLDVIGANYNTTRLI
                     ITPADDSTIPPTPAVMESDTDYRLRIQQAFEGLSVAGSVGAYQYHGRSADGRVADISV
                     TSPSPACVTISVLSRENNGVASEDLLAVVRNALNGEDVRPVADRVTVQSAAIVEYQIN
                     ATLYLYPGPESEPIRAAAVKKLEAYITAQHRLGRDIRLSAIYAALHVEGVQRVELTAP
                     LADIVLNSTQASFCTEYSVVTGGSDE"
     gene            complement(2334..2681)
                     /locus_tag="N7B39_003229"
     CDS             complement(2334..2681)
                     /locus_tag="N7B39_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HGU2423162.1"
                     /translation="MTARYLGMNRSDGLTVTDLEHISQSIGDILRTPVGSRVMRRDYG
                     SLLALMIDQPQTPALELQIKVACYMAVLKWEPRVTLSSVTTERSFDGRMTVTLTGQHN
                     DTGQPLSLTIPVS"
     gene            complement(2678..3313)
                     /locus_tag="N7B39_003230"
     CDS             complement(2678..3313)
                     /locus_tag="N7B39_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HGU2423163.1"
                     /translation="MNTLANIQELARALRNMIRTGLVVETDLNAGRCRVQTGGMCTDW
                     LQWLTCRAGRSRTWWAPSVGEQVLILAVGGELDTAFVLPGIYSGDNPAPSASADALHI
                     RFPDGAVIEYEPETSALTVSGIKTASVTASDSVTATVPVVMVKASTRITLDTPEVVCT
                     NRLITGTLEVQKGGTMRGNIEHTGGELSSNGKVLHTHKHPGDSGGTTGSPL"
     gene            complement(3380..3832)
                     /locus_tag="N7B39_003231"
     CDS             complement(3380..3832)
                     /locus_tag="N7B39_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HGU2423164.1"
                     /translation="MNEFKRFEDRLTGLTESLSPSGRRRLSAELAKRLRQSQQRRVMT
                     QKAPDGTPYAPRQQQSVRKKTGRVKRKMFAKLITSRFLHIRASQEQASMEFYGGKSPK
                     IASVHQFGLSEENRKDGKKIDYPARPLLGFTGEDVQMIEEIILAHLDR"
     gene            complement(3825..4292)
                     /locus_tag="N7B39_003232"
     CDS             complement(3825..4292)
                     /locus_tag="N7B39_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001406878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU2423165.1"
                     /translation="MLKPDSLRRALTDAVTVLKTNPDMLRIFVDNGSIASTLAASLSF
                     EKRYTLNVIVTDFTGDFDLLIVPVLAWLRENQPDIMTTDAGQKKGFTFYADINNDSSF
                     DISISLMLTERTLVSEVVGALHVKNIPEPPPPEPVTRPMELYINGELVSKWDE"
     gene            complement(4255..4500)
                     /gene="lysC"
                     /locus_tag="N7B39_003233"
     CDS             complement(4255..4500)
                     /gene="lysC"
                     /locus_tag="N7B39_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979584.1"
                     /note="LysC is an Rz1-like component of a phage lytic
                     system, substantially overlapping although not fully
                     embedded in the gene for the Rz-like LysB component;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1-like lysis system protein LysC"
                     /protein_id="HGU2423166.1"
                     /translation="MLCAGCTSAPPAQTPVIVANACPKVSLCPMPGSDPQTNGDLSAD
                     IRQLENALARCASQVKMIKHCQDENDAQTRQPAQGAD"
     gene            complement(4400..4825)
                     /gene="lysB"
                     /locus_tag="N7B39_003234"
     CDS             complement(4400..4825)
                     /gene="lysB"
                     /locus_tag="N7B39_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585210.1"
                     /note="The gene for this Rz-like phage lysis system
                     protein may overlap extensively with the gene for the
                     other spanin subunit, the Rz1-like protein in the outer
                     membrane; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz-like lysis system protein LysB"
                     /protein_id="HGU2423167.1"
                     /translation="MSKLMIVLVVLLSLAVAGLFLAKHENASLRASLDRANNVASGQQ
                     TTIIMLKNQLHVALTRADKNEMAQVALRQELENAAKREAQREKAITRLLNENEDFRRW
                     YGTDLPDAVRRLHQRPACADASDCRQRLPESEPLPDAGQ"
     gene            complement(4813..5238)
                     /locus_tag="N7B39_003235"
     CDS             complement(4813..5238)
                     /locus_tag="N7B39_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNZ54_00345 family protein"
                     /protein_id="HGU2423168.1"
                     /translation="MKKLSLSLMLHVSLALMLALSLIYPQSVAVSFVAAWAILATVIC
                     VVAGGVGVYATEYVLERYGRELPPESLAVKIVTSLFLQPVPWRRRAAALVVMVATFIS
                     LVAAGWIFTALIYLVASLFFRLIRTACRQRFEGREPCQS"
     gene            complement(5253..5750)
                     /locus_tag="N7B39_003236"
     CDS             complement(5253..5750)
                     /locus_tag="N7B39_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 104 protein"
                     /protein_id="HGU2423169.1"
                     /translation="MPVINTHQNIAAFLDMLAVSEGTANHPLTKNRGYDVIVTGLDGK
                     PEIFTDYSDHPFAHGRPAKVFNRRGEKSTASGRYQQLYLFWPHYRKQLALPDFSPLSQ
                     DRLAIQLIRERGALDDIRAGRIERAISRCRNIWASLPGAGYGQREHSLEKLVTVWRTA
                     GGVPA"
     gene            complement(5750..6031)
                     /locus_tag="N7B39_003237"
     CDS             complement(5750..6031)
                     /locus_tag="N7B39_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HGU2423170.1"
                     /translation="MTAEEKSVLSLFMIGVLIVVGKVLAGGEPITPRLFIGRMLLGGF
                     VSMVAGVVLVQFPDLSLPAVCGIGSMLGIAGYQVIEIAIQRRFKGRGKP"
     gene            complement(6035..6238)
                     /locus_tag="N7B39_003238"
     CDS             complement(6035..6238)
                     /locus_tag="N7B39_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HGU2423171.1"
                     /translation="MKTFALQGDTLDAICVRYYGRTEGVVETVLAANPGLAELGAMLP
                     HGTAVELPDVQTAPVAETVNLWE"
     gene            complement(6238..6747)
                     /locus_tag="N7B39_003239"
     CDS             complement(6238..6747)
                     /locus_tag="N7B39_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HGU2423172.1"
                     /translation="MMTLIIPRKEAPVSGEGTVVIPQPAGDEPVIKNTFFFPDIDPKR
                     VRERMRLEQTVAPARLREAIKSGMAETNAELYEYREQKIAAGFTRLADVPADDIDGES
                     IKVFYYERAVCAMATASLYERYRGVDASAKGDKKADSIDSTIDELWRDMRWAVARIQD
                     KPRCIVSQI"
     gene            complement(6847..7590)
                     /locus_tag="N7B39_003240"
     CDS             complement(6847..7590)
                     /locus_tag="N7B39_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HGU2423173.1"
                     /translation="MTSPAQRHMMRVSAAMTAQRDAAPLRHATVYEQMLVKLAADQRT
                     LKAIYSKELKAAKKRELLPFWLPWVNGVLEQGKGAQDDILMTVMLWRLDTGDIAGALE
                     IARYALKYGLTMPGKHRRTPPYMFTEEVALAAMRAHAAGESVDTRLLTETLALTATAD
                     MPDEVRAKLHKITGLFLRDAGDAAGALAHLQRATQLDCQAGVKKEIERLERELKPKPE
                     PQPKAATRAPRKTRSVTPAKRGRPKKKAS"
     gene            complement(7594..8667)
                     /locus_tag="N7B39_003241"
     CDS             complement(7594..8667)
                     /locus_tag="N7B39_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HGU2423174.1"
                     /translation="MRQETRFKFNAYLSRVAELNGIDAGDVSKKFTVEPSVTQTLMNT
                     MQESSDFLTRINIVPVSEMKGEKIGIGVTGSIASTTDTAGGTERQPKDFSKLASNKYE
                     CDQINFDFYIRYKTLDLWARYQDFQLRVRNAIIKRQSLDLIMAGFNGVRRAETSDRSS
                     NQMLQDVAVGWLQKYRNEAPARVMSKVTDEEGHTTSEVIRVGKGGDYASLDALVMDAT
                     NNLIEPWYQEDPDLVVIVGRQLLADKYFPIVNREQDNSEMLAADVIISQKRIGNLPAV
                     RVPYFPADAMLITKLENLSIYYMDDSHRRVIVENPKLDRVENYESMNIDYVVEDYAAG
                     CLVEKIKVGDFSTPTKVTAEPGA"
     gene            complement(8726..9580)
                     /locus_tag="N7B39_003242"
     CDS             complement(8726..9580)
                     /locus_tag="N7B39_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HGU2423175.1"
                     /translation="MAKKVSKFFRIGVEGDTCDGRVISAQDIQEMAETFDPRVYGCRI
                     NLEHLRGILPDGIFKRYGDVAELKAEKIDDDSALKGKWALFAKITPTDDLIAMNKAAQ
                     KVYTSMEIQPNFANTGKCYLVGLAVTDDPASLGTEYLEFCRTAKHNPLNRFKLSPENL
                     ISVATPVELEFEDLPETVFTALTEKVKSIFGRKQASDDARLNDVHEAVTAVAEHVQEK
                     LSATEQRLAEMETAFSALKQEVTDRADETSQAFSRLKNSLDHTESLTQQRRSKATGGG
                     GDALMTNC"
     gene            9754..11526
                     /locus_tag="N7B39_003243"
     CDS             9754..11526
                     /locus_tag="N7B39_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HGU2423176.1"
                     /translation="MTITTDTTLLHDPRRQAALLYWQGFSVPQIAAMLQMKRPTVQSW
                     KQRDGWDSVAPISRVEMSLEARLTQLIIKPQKTGGDFKEIDLLGRQIERLARVNRYSQ
                     TGNEADLNPNIANRNKGGRRKPKKNFFSDEAIEKLEQIFFEQSFEYQLHWYRAGLEHR
                     IRDILKSRQIGATFYFSREALLRALKTGHNQIFLSASKTQAYVFREYIIAFARLVDVD
                     LTGDPIVLGNNSAKLIFLGTNSNTAQSHNGDLYVDEIFWIPNFQVLRKVASGMASQSH
                     LRSTYFSTPSTLAHDAYPFWSGELFNRGRASAAERVEIDVSHNALAGGLLCADGQWRQ
                     IVTIEDALKGGCTLFDIEQLKRENSADDFKNLFMCEFVDDKASVFPFEELQRCMVDTL
                     EEWEDYAPFAANPFGSRPVWIGYDPSHRGDSAGCVVLAPPVVAGGKFRILERHQWKGM
                     DFATQAESIRKLTEKYNVEYIGIDATGLGVGVFLLVRSFYPAARDIRYTPEMKTAMVL
                     KAKDVIRRGCLEYDVSATDITSSFMAIRKTMTSSGRSATYEASRSEEASHADLAWATM
                     HALLNEPLTAGISTPLTSTILEFY"
     gene            11526..12560
                     /locus_tag="N7B39_003244"
     CDS             11526..12560
                     /locus_tag="N7B39_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001752368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU2423177.1"
                     /translation="MSKKKGKTPQPAAKKMTASAPKMEAFTFGEPVPVLDRRDILDYV
                     ECISNGRWYEPPVSFTGLAKSLRAAVHHSSPIYVKRNILASTFIPHPWLSQQDFSRFV
                     LDFLVFGNAFLEKRYSTTGKVIRLETSPAKYTRRGVEEDVYWWVPSFNEPTAFAPGSV
                     FHLLEPDINQELYGLPEYLSALNSAWLNESATLFRRKYYENGAHAGYIMYVTDAVQDR
                     NDIEMLRENMVKSKGRNNFKNLFLYAPQGKADGIKIIPLSEVATKDDFFNIKKASAAD
                     LLDAHRIPFQLMGGKPENVGSLGDIEKVAKVFVRNELIPLQDRIREINGWLGQEVIRF
                     KNYSLDTDNG"
BASE COUNT         2711 a         3721 c         3475 g         2801 t
ORIGIN      
        1 cgagaagacc ggcaacatcc tttccactca aatgagtcag cgtctcatcc agtggctgct
       61 tacctgacag cgcattgtta atggtggtgc tgaatttcgg gtcattgtta atggccgcgg
      121 caatttcttt cagtgtgtcc agcgtgtctg gcgcgccgtt aatcagagcg gtaatagcgg
      181 tctgaacaaa ctcagtggtc gcaatccgcg tggtgttatt tcctgcggca ggcgttggcg
      241 cttttggttc tccggtaaat gtcggattat gtttctgcgc atactgggta tgaggatcct
      301 gtgcggcaat gtggtttctc atctggtcat ccacatacag ccttaattcc aggactttat
      361 catccacgta tttacgggtc gccagtacca ccgacgggtc gattttcagc gtgatggctt
      421 cggtattcgt gacaaccaga atcatgcgga tagtctgggt acgaccgctg ccttcctgta
      481 actgcggttt gtacgtttcc gggcagttcg ccaccgcaat gagtacgcct tcatcatcat
      541 aaagcccaat ctcacggatc cagaatccgc cctcgttctc agggatgatt tgctccgcaa
      601 taatctggct ctggttgttc gggtcaacac tcagaagatt cagcggcgcg atgcgtttct
      661 ggttaataag ttttgtctgt gccgggtcag gtgtcggcaa gacaccattt gcatcaccaa
      721 cggccatttg cgtcagattc agcttactgc cgagcatcgt cgcgttagcc agccgtgccg
      781 cgccctgatt agtcagaatg gcgtagtatt tcactgtcat gcgtttactc tcaggttatc
      841 aattaaatga atggccgagg ccgggaaata atcccctccg acaataatgg cctccggggt
      901 gtagggataa accgtcaggg cgtcgccgtg atagcatccc gcaccggcaa aaatgttgcc
      961 ggttgtactt aaactgatag ccagtcccgt cagatggcgg cttgcaggtt ttgcatcagc
     1021 aacgagacgc tccagctcct gatacatttc ctcggtaata ccctgctcaa gcacgccaac
     1081 aacgatgcgg aacgtccccg gctcctcgtt gagctgccac cactccctca cctcaatcag
     1141 atagccgagc ggctccacca cacggcgaat cgcaccaata gtgcccttat ggcagtgaat
     1201 gaaatacgca tcgcggataa cagcgcgttt tgtcgcttcc ggccacttat catcccagcg
     1261 gtcaaccgaa aatgaccacg ccagccacgg cagcagattt gccggacagg tatccgggtt
     1321 ccacagctca cgaatactga ccggcgtttt ttcaatttcc gcacaggctc ttgcggcggc
     1381 gacttcaagc ggtgatgagc cggtcggcag caggcgcgaa tcactcatcc gagcctccgg
     1441 tcacgacgct gtattcggtg cagaaagacg cctgcgtact gttgagcaca atgtcagcca
     1501 gaggtgcagt cagttcgaca cgttgcacgc cttccacatg caaagcggca taaatggcag
     1561 acagacggat gtcgcgccca agccggtgct gtgccgtgat gtacgcttcc agttttttca
     1621 cggcagcagc gcggatgggt tcgctttcgg gacctgggta aaggtaaagc gtggcgttta
     1681 tctgatattc aacaatggcg gcagactgca cggtcacgcg gtcggccacc ggcctgacgt
     1741 cctcgccatt aagggcgtta cgcaccacgg ccagcaggtc ttcggatgcg acgccgttat
     1801 tttcacgtga cagcacggag atagtgacgc aggccggaga cggactggtg acagagatat
     1861 ccgcgacacg cccgtcagca ctgcgaccat gatactgata ggctcccacc gacccggcga
     1921 cgcttaagcc ctcaaacgcc tgctgaatac gcagacgata atcggtgtca gactccatca
     1981 ctgccggtgt cggcgggata gtcgaatcat ctgccggggt gataatcagg cgtgtggtgt
     2041 tgtaattggc accaatcaca tcaaggtcat taccggcggc acaggccagc atcaccgccc
     2101 gtgcggcctc attcacacgc tgacgccaga taagctcacg ataagcattt tcctccagaa
     2161 gtttgacgag aggctcggat tccagcgtca gggtacgggc gaccgcctcc tgctggtctt
     2221 ccgggtaaag ggaaatcagt gtcgccttgc gttcggcaag aatggtttca aagtccagct
     2281 cctcgaccac atccggtgcg ggtagctggt tcaggtcgat aatcggcatg gtttcaactc
     2341 acagggatgg ttaacgaaag tggctggccg gtgtcgttgt gctggccggt taacgtgacc
     2401 gtcattcgcc cgtcaaaact gcgctcagta gtgacggatg acagggtgac gcggggttcc
     2461 catttcagca ccgccatgta acaggcgacc ttaatctgca actcaagcgc cggggtctgc
     2521 ggctggtcaa tcattaatgc cagcaacgag ccgtaatcac gacgcatcac ccgtgagccg
     2581 accggtgtac gcaggatatc gccgatactc tggctgatat gctcaaggtc agtgacagtc
     2641 aggccatcac tgcgattcat tccgagataa cgcgctgtca taaaggactc ccggttgtgc
     2701 cgccgctgtc gccggggtgt ttatgggtat gcagtacctt accgtttgat gagagttcac
     2761 caccggtgtg ttcaatgttg ccgcgcatcg tcccgccctt ctgtacttcc agcgtgccgg
     2821 taatcagcct gttggtgcag accacctccg gtgtgtccag ggtgatgcgg gttgacgctt
     2881 tcaccatgac caccggcacc gtggcagtaa cagaatcaga agccgtcacg ctggccgttt
     2941 taattccgct taccgtcagt gcactggttt caggttcata ctcaatcacc gccccgtcag
     3001 ggaaacggat atgcagggca tccgccgacg cagacggcgc ggggttatcg ccggaataaa
     3061 tccccggcag aacaaacgct gtgtcaagtt caccgcccac ggccagaatc agcacctgct
     3121 cccccacgga aggtgcccac catgtgcgcg aacgcccggc acgacaagtc agccactgaa
     3181 gccagtcggt gcacatgccg ccggtctgca cacggcagcg accggcgtta aggtcggttt
     3241 cgacgacaag gccggtgcga atcatgttgc gcagtgcgcg cgcgagttcc tggatatttg
     3301 cgagagtgtt cataacggga aggatgccgc cgggtcatac cggcggcaat gtgacgatga
     3361 ggtgtcagga atggcacaac taacggtcga ggtgagccag gataatctct tcaatcatct
     3421 gcacatcctc accggtaaag ccgagcagag gacgcgccgg ataatcaatt ttcttaccgt
     3481 ctttccggtt ttcttccgac agaccgaact gatgcacgct ggcgattttc ggtgacttcc
     3541 cgccgtaaaa ctccattgat gcctgttcct ggctggcacg gatatgcaaa aaacgactgg
     3601 taataagttt cgcaaacatt tttcgcttaa cgcgaccggt cttttttctg acgctctgct
     3661 gctggcgtgg cgcgtagggt gtgccgtccg gggctttctg tgtcatcacc cgacgctgct
     3721 gactctgccg cagacgtttc gccagttcgg cgctcagtcg tcgacgccct gacggtgaca
     3781 gcgattcagt cagtccggtc agccggtctt caaaacgctt aaactcattc atcccacttg
     3841 ctcaccagtt cgccattgat ataaagctcc atcgggcggg tgaccggctc cggcggcgga
     3901 ggttccggga tattcttcac atgcagtgcg ccgaccacct cactgaccag cgtgcgctcg
     3961 gtcagcatca ggctgatgct gatatcaaag ctgctgtcat tgttgatgtc tgcataaaac
     4021 gtgaagccct ttttctggcc tgcgtcggtg gtcatgatgt cgggctgatt ttcccgcagc
     4081 cacgccagca ccggcacgat gagcaggtca aaatcaccgg taaagtcggt cacaatgaca
     4141 ttgagcgtgt aacgcttttc gaatgacagc gacgccgcca gtgtggaggc aatactcccg
     4201 ttatccacga atatccgcag catatcgggg ttagttttca gcaccgtgac ggcatcagtc
     4261 agcgccctgc gcaggctgtc gggtttgagc atcgttttcg tcctgacagt gtttaatcat
     4321 ttttacctgg ctggcacagc gtgccagcgc gttctcaagc tgccggatat cagcacttaa
     4381 atcgccgttc gtctgcgggt cactgcccgg catcgggcaa aggctcactt tcgggcaggc
     4441 gttggcgaca atcactggcg tctgcgcagg cggggcgctg gtgcaaccgg cgcacagcat
     4501 caggcaggtc agtgccgtac cagcggcgaa aatcttcgtt ttcattgagt aacctcgtga
     4561 tggctttctc gcgctgtgct tcacgcttcg cggcgttctc cagctcctga cgcagtgcca
     4621 cctgcgccat ctcgtttttg tctgccctgg tgagggcaac atgaagctga tttttcagca
     4681 tgatgatggt cgtctgctgc ccactggcga cattgtttgc cctgtccagc gaggcgcgca
     4741 ggctggcgtt ttcatgcttc gccagaaaca gaccggccac cgccagtgat aacaacacaa
     4801 ccagcacaat catcagcttt gacatggttc ccgcccctca aaacgctgac ggcaggccgt
     4861 acgtatcagc cggaaaaaca gcgacgccac aagataaatc agcgcggtaa aaatccaccc
     4921 ggcggcgacc agcgagataa acgtcgccac catcaccacc agagccgccg cccgtctgcg
     4981 ccacggcacc ggctgcaaaa acagcgacgt gacaatcttc acggccagcg attccggcgg
     5041 cagctcccgc ccgtagcgtt ccagtacata ctctgtggca tacaccccga caccaccggc
     5101 aaccacacag ataaccgtcg ccagaatcgc ccaggcagcg acaaaactga cggccacgct
     5161 ctgcggataa atcagggaca gtgccagcat cagcgccagc gacacgtgca gcatcagtga
     5221 aagggataat ttcttcatgg tgtttactcc gtttaagccg gtacgccgcc agcggtacgc
     5281 cagacggtga ccagtttttc cagtgaatgc tcacgctgac cgtaaccggc acccggcagg
     5341 gacgcccaga tattgcgaca gcgtgaaatg gcacgctcaa tgcgtcccgc ccggatgtca
     5401 tccagtgcac cgcgttcgcg gatcaactga atggcgagcc tgtcctgtga caacggactg
     5461 aaatccggca gggcaagctg tttgcggtaa tgcggccaga acaggtaaag ctgctgatag
     5521 cgaccggagg ccgtggattt ttcaccgcga cggttaaaca ccttcgccgg tcggccatgc
     5581 gcgaacgggt ggtcactgta gtcggtgaaa atttccggct ttccgtccag tccggtgact
     5641 atcacgtcat agccccggtt tttcgtcagc ggatgattcg ccgtcccttc ggacacggcc
     5701 agcatgtcga gaaaggcggc gatattctga tgcgtgttaa ttaccggcat tacggtttcc
     5761 ccctgccctt aaagcggcgc tgaatggcaa tctcaatcac ctgataaccg gcgataccca
     5821 gcatggagcc gataccgcac accgcaggca gtgacaggtc aggaaactgc accagaacaa
     5881 caccggcaac catcgagaca aaaccaccga gcaacatgcg cccgataaac agacgcgggg
     5941 tgatgggttc accaccggca agcaccttgc cgacaacaat cagcactcca atcatgaaaa
     6001 gcgacaggac gcttttttct tctgctgtca tgcgttactc ccacagattg acagtttcag
     6061 ccacgggcgc ggtctgaacg tcgggcagtt cgacggcggt gccgtgcggc agcatcgcac
     6121 ccagttcagc cagtcccgga tttgcggcga gcacggtctc aaccacgccc tcagtgcgcc
     6181 cgtaataccg gacacaaatg gcgtcgagcg tgtcgccctg tagcgcaaag gtcttcatca
     6241 gatttgactc acgatgcagc gcggcttgtc ctggatacgt gccaccgccc agcgcatatc
     6301 ccgccacagt tcatcaatgg tgctgtcaat gctgtcggcc ttcttgtcgc ccttggcact
     6361 ggcatccacg ccgcgataac gctcataaag cgatgcggtc gccatcgcac acacggcgcg
     6421 ctcgtagtaa aaaactttga tgctttcacc gtcgatgtcg tccgccggga cgtccgccag
     6481 acgcgtaaaa ccggcagcaa ttttctgttc gcggtactcg tacagctccg cattcgtttc
     6541 cgccatgcct gacttgatgg cctcacgcag acgggcgggg gcgacggtct gctcaaggcg
     6601 catacgttcc cggacgcgct tcgggtcgat atcgggaaaa aagaacgtgt ttttaatcac
     6661 cggctcgtcg cctgccggtt gcgggatgac caccgtaccc tcaccggaca cgggagcctc
     6721 ctttcgcgga ataatcagcg tcatcatgac tacctctgaa aagtcgggcg gtggacgccg
     6781 gtgcagtgtc aggtgattca ccctcactga ccggcgtgcc gccctggcgc ggggcgcatt
     6841 cggttgttaa ctggctttct ttttcgggcg tccacgtttt gccggtgtca cgctccgggt
     6901 cttacgcggg gcgcgggtgg ccgctttggg ctgcggctcc ggcttcggtt tcagctcccg
     6961 ctccagtcgt tcaatctctt ttttgacgcc tgcctgacag tcgagctgtg tcgcacgttg
     7021 caggtgagcc agcgcaccgg cggcatcacc agcgtcacgc agaaacagac cggtgatttt
     7081 gtgcagcttt gcacgcactt catcaggcat gtctgccgtg gcggtcagtg caagggtctc
     7141 cgtcagcagg cgggtatcca cagactcacc ggcagcgtgg gcgcgcatgg ccgcgagcgc
     7201 cacctcctcg gtgaacatat acggcggggt gcggcggtgt ttacccggca tggtcagacc
     7261 gtacttcagg gcataacggg caatctccag cgcaccggca atatcgccgg tatccagacg
     7321 ccacagcatg accgtcatca gaatgtcatc ctgtgcacct ttgccctgct ccagcacgcc
     7381 gttcacccac ggcaaccaga acggcagcag ttcgcgtttt ttcgcggcct tcagctcttt
     7441 tgaataaatc gctttcagtg tgcgctggtc tgcggccagc ttgaccagca tctgctcata
     7501 gacagttgca tgtcgcagcg gggcggcgtc ccgctgcgcg gtcatcgctg ccgagacccg
     7561 catcatgtgg cgctgtgcgg gactcgtcat cggttacgct cccggctctg cggtcacttt
     7621 agtcggtgtg gagaagtcac cgaccttaat tttttccacc agacaaccgg cggcgtagtc
     7681 ttccaccacg taatcaatgt tcattgactc gtagttctcc acgcggtcga gtttcgggtt
     7741 ttccacaatc acgcggcgat ggctgtcatc catgtagtag atggacaggt tttccagctt
     7801 cgtgatgagc atcgcatccg ccgggaagta cgggacgcgt accgccggca ggttaccgat
     7861 gcgtttctgg ctgatgatga cgtcagcggc cagcatctcg ctgttgtcct gctccctgtt
     7921 gacgatggga aaatacttgt ccgccagcag ctgacgcccc acaatcacca caaggtcagg
     7981 gtcttcctga taccacggct caatcaggtt gttggtcgca tccatcacca gtgcatcgag
     8041 gctggcataa tcaccgccct tacccacgcg gatgacctca gaggtcgtgt gaccttcctc
     8101 gtcagtaacc ttgctcatca cgcgcgccgg ggcttcattg cggtatttct gcagccagcc
     8161 gaccgccaca tcctgcagca tctggttact gctgcggtca gaggtttcgg cacgcctcac
     8221 gccgttaaaa ccggccatga ttaaatcaag ggactggcgt ttgataatgg cgttacggac
     8281 acggagctgg aaatcctgat aacgcgccca caggtcaagc gttttgtagc ggatataaaa
     8341 atcgaagtta atctggtcgc attcgtactt gtttgacgcc agcttcgaga agtccttcgg
     8401 ctgacgctcg gtgccaccgg cggtgtctgt ggtgctggcg atggagccgg tgacaccaat
     8461 accaattttt tcccctttca tttcgctgac cggcacaatg ttgatgcggg tcagaaagtc
     8521 agaggactcc tgcatggtgt tcatcagggt ctgggtgacc gacggttcaa cggtgaattt
     8581 tttcgacaca tcaccggcgt cgatgccgtt cagttcggca acacgggaca ggtaggcatt
     8641 aaatttaaag cgggtttcct ggcgcatagt ttttcctgaa attaagggtt aatcgtgaag
     8701 gttttcccgg actggctgac gccggtcagc agttcgtcat cagggcgtca ccgccaccgc
     8761 cggtggcctt gctgcggcgc tgctgggtca gactttcggt gtggtcgaga ctgtttttca
     8821 ggcggctgaa tgcctggctg gtttcatccg ccctgtcagt cacctcctgc ttaagtgcgg
     8881 aaaaggcggt ttccatctca gcgagtcgct gctcagtggc gctcagcttt tcctgcacat
     8941 gttcagcaac agcggtcacc gcttcatgca cgtcattcag acgggcgtca tcgctggcct
     9001 gtttgcggcc aaaaatggat ttcacctttt cggtcagggc ggtgaacacg gtttcaggca
     9061 ggtcttcaaa ttccagctca acaggcgttg ctactgaaat caggttttca gggcttaatt
     9121 tgaagcggtt cagggggttg tgttttgccg tgcggcagaa ttccaggtat tccgtgccga
     9181 ggcttgccgg gtcatcggtg acggccagac ccaccagata acatttgccg gtgttggcaa
     9241 agttcggctg aatttccatt gaggtgtaga ccttctgcgc ggccttgttc atcgcgataa
     9301 ggtcatcggt cggggtgatt ttcgcaaaca gcgcccattt gcctttcagc gccgaatcgt
     9361 catcaatctt ttcggccttc agttcggcca catcgccata acgtttaaaa ataccgtcag
     9421 gcaggatgcc gcgcagatgt tccaggttaa tgcggcaacc atagacacgc gggtcaaagg
     9481 tttcggccat ttcctgaata tcctgcgcac tgatgacacg cccgtcacag gtgtcaccct
     9541 caacgccgat acgaaagaat tttgagactt tttttgccat tgtcaggagt cctgaatagt
     9601 gattagaaga gtcacatgtc ggcatcagtt tcccgacgat gcgcatcctc cgccatcagt
     9661 cccggatggc ttatcactga cacaacagca ccttagcgaa tcgcggggcg cgactcagta
     9721 gccttgccgt gtattcatca cggcgaggta ttcatgacca tcaccacaga caccactctt
     9781 ttacacgacc cgcgtcgtca ggcggcgctg ctgtactggc agggattttc cgtgccgcag
     9841 attgccgcca tgttgcagat gaaacgcccg acggtgcaga gctggaaaca gcgcgacggc
     9901 tgggacagcg ttgcccccat cagccgtgtc gaaatgagtc tggaagcgcg gctgacccag
     9961 ctcatcatca aaccgcagaa aaccggcggt gacttcaagg aaattgacct gctcggacgc
    10021 cagattgaac gactggcacg ggtaaaccgc tacagccaga ccggcaacga ggcagacctt
    10081 aatccgaaca tcgctaaccg caacaaaggc gggcggcgca aaccgaaaaa gaattttttc
    10141 agtgacgagg ctatcgaaaa gctggagcag attttctttg agcagtcttt cgaatatcag
    10201 ttgcactggt atcgcgccgg gcttgagcac cgcatccgcg atatcctgaa atcccgccag
    10261 attggcgcga cgttttattt ttcccgcgag gcgctgctgc gcgccctgaa aaccggtcat
    10321 aaccagattt ttctgtcggc cagtaaaacg caggcgtatg tgttccgcga atacatcatc
    10381 gcctttgccc ggctggttga cgttgacctg accggtgacc cgattgtcct gggcaataac
    10441 agcgcaaaac tgatttttct cggcaccaac tccaacaccg cgcagagcca taacggcgac
    10501 ctgtacgtcg acgagatttt ctggataccg aattttcagg tactgcgtaa ggtggcatca
    10561 ggtatggcct cacagagtca cctgcgctcg acctatttct ccaccccgtc cacgctggcg
    10621 cacgacgcct acccgttctg gtcgggtgaa ctgttcaacc ggggacgcgc cagcgccgcc
    10681 gaacgcgtgg aaatcgacgt cagtcataac gctcttgccg gtgggcttct ctgtgcggac
    10741 ggtcagtggc ggcagattgt caccattgag gacgccctga aaggtggctg cacgctgttc
    10801 gacattgagc agcttaaacg cgaaaacagc gccgacgatt ttaaaaacct gttcatgtgt
    10861 gaatttgttg acgacaaggc gtcggtgttc ccgttcgagg agctgcaacg ctgcatggtc
    10921 gacacgctgg aagaatggga agactatgcg ccgtttgccg cgaatccgtt cggctcccgc
    10981 ccggtctgga ttggttacga cccgtcacac cgtggcgaca gtgccggatg cgtggtgctg
    11041 gcaccgccgg tggtggccgg tggcaaattc agaatacttg agcgtcacca gtggaaaggc
    11101 atggactttg ccacccaggc tgaatccatc cgcaaactca ccgaaaaata caacgtcgaa
    11161 tacatcggaa ttgatgccac cggcctcggt gtcggcgtgt tcctgctcgt tcgctcgttc
    11221 tatcccgccg cacgcgatat ccgctacacg ccggaaatga aaaccgcaat ggtgctcaag
    11281 gcaaaagacg ttattcgccg tggctgtctg gaatatgacg tcagcgccac cgacatcacc
    11341 agctcgttta tggctatccg caagaccatg accagcagcg gacgcagcgc cacctatgag
    11401 gccagccgca gcgaggaagc cagccacgcc gacctcgcct gggcgaccat gcacgccctg
    11461 ttaaatgagc cactcaccgc cggtatcagc accccgctga catccaccat tctggagttt
    11521 tactgatgag caagaaaaaa gggaaaacac cgcaacctgc ggcaaaaaaa atgaccgcca
    11581 gcgccccgaa aatggaggca ttcacctttg gtgaaccggt gccggtactc gaccgccgtg
    11641 acattctgga ttacgtcgag tgcatcagta acggcagatg gtatgagcca ccggtcagct
    11701 ttaccggtct ggcaaaaagc ctgcgtgctg ccgtacatca cagctcaccg atttacgtca
    11761 aacgtaatat tctggcttca acgtttattc cgcacccgtg gctttcccag caggatttca
    11821 gccgctttgt gctggatttt ctggtgttcg gtaatgcgtt tctggaaaag cgttacagca
    11881 ccaccggtaa ggtcatcaga ctggaaacct caccggcaaa atatacccgc cgtggcgtgg
    11941 aggaggatgt ttactggtgg gtgccgtcct tcaacgagcc gacagccttc gcgcccggct
    12001 ccgtgtttca cctgctggag ccggatatta atcaggagct gtacggcctg ccggaatatc
    12061 tcagcgccct taattctgcc tggctgaatg agtcggccac gctgttccgc cgcaagtatt
    12121 acgaaaacgg cgctcatgcc ggatatatca tgtacgtcac cgatgccgtg caggatcgca
    12181 acgatatcga aatgcttcgc gaaaacatgg tcaagtcgaa aggccgcaac aactttaaaa
    12241 atctgtttct ctatgccccg caggggaaag ccgacggcat taaaattatc ccgctcagtg
    12301 aagtggcaac gaaggacgat ttttttaata tcaaaaaagc cagcgccgct gacctgctgg
    12361 acgcgcaccg catccccttt cagttgatgg gcggcaagcc ggagaacgtc gggtcgctgg
    12421 gtgatattga gaaagtggca aaggtctttg tccgcaatga gcttatcccg ctacaggaca
    12481 ggatccgcga gataaacggc tggctcggtc aggaggtcat ccgctttaaa aactactcac
    12541 tggacactga caacggctga acatcgccgc ctgcgggcgg cttttttaca ccccgtcatc
    12601 acgccctcac acgctcacca ccgcacaaaa caccccgcag acacaccaac gcctcaacgg
    12661 gcagactaag cgccatcacg acgcgctcag acgctgaaaa aataaaat
//
LOCUS       DBFIFI010000123        12689 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.123, whole genome shotgun sequence.
ACCESSION   DBFIFI010000123 DBFIFI010000000
VERSION     DBFIFI010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12689
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.123"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            69..1124
                     /gene="phoE"
                     /locus_tag="N7B39_003245"
     CDS             69..1124
                     /gene="phoE"
                     /locus_tag="N7B39_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HGU2423178.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(1163..1564)
                     /gene="crl"
                     /locus_tag="N7B39_003246"
     CDS             complement(1163..1564)
                     /gene="crl"
                     /locus_tag="N7B39_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HGU2423179.1"
                     /translation="MTLPSGHPKSRLIKKFTVLGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(1622..2866)
                     /gene="frsA"
                     /locus_tag="N7B39_003247"
     CDS             complement(1622..2866)
                     /gene="frsA"
                     /locus_tag="N7B39_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HGU2423180.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSCLLHQRVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            complement(2958..3416)
                     /gene="gpt"
                     /locus_tag="N7B39_003248"
     CDS             complement(2958..3416)
                     /gene="gpt"
                     /locus_tag="N7B39_003248"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HGU2423181.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3677..5134
                     /gene="pepD"
                     /locus_tag="N7B39_003249"
     CDS             3677..5134
                     /gene="pepD"
                     /locus_tag="N7B39_003249"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HGU2423182.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(5191..5712)
                     /gene="prfH"
                     /locus_tag="N7B39_003250"
                     /pseudo
     CDS             complement(5191..5712)
                     /gene="prfH"
                     /locus_tag="N7B39_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(5711..5914)
                     /locus_tag="N7B39_003251"
                     /pseudo
     CDS             complement(5711..5914)
                     /locus_tag="N7B39_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            complement(6233..6685)
                     /gene="yafP"
                     /locus_tag="N7B39_003252"
     CDS             complement(6233..6685)
                     /gene="yafP"
                     /locus_tag="N7B39_003252"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU2423183.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFIRAVMMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAQPVGFISRIEHYIDMLFVDPEYTRRGVASALLKPWIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFTNFSMRYKPQH"
     gene            complement(6695..7093)
                     /gene="yafO"
                     /locus_tag="N7B39_003253"
     CDS             complement(6695..7093)
                     /gene="yafO"
                     /locus_tag="N7B39_003253"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HGU2423184.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDESHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            complement(7096..7389)
                     /gene="yafN"
                     /locus_tag="N7B39_003254"
     CDS             complement(7096..7389)
                     /gene="yafN"
                     /locus_tag="N7B39_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HGU2423185.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAEKYLNDMTDDDFNDFKE"
     gene            complement(7441..8496)
                     /gene="dinB"
                     /locus_tag="N7B39_003255"
     CDS             complement(7441..8496)
                     /gene="dinB"
                     /locus_tag="N7B39_003255"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HGU2423186.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFSELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVSAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(8567..9352)
                     /gene="lafU"
                     /locus_tag="N7B39_003256"
     CDS             complement(8567..9352)
                     /gene="lafU"
                     /locus_tag="N7B39_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HGU2423187.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            9324..11036
                     /locus_tag="N7B39_003257"
                     /pseudo
     CDS             9324..11036
                     /locus_tag="N7B39_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140831.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(11181..11678)
                     /gene="rayT"
                     /locus_tag="N7B39_003258"
                     /pseudo
     CDS             complement(11181..11678)
                     /gene="rayT"
                     /locus_tag="N7B39_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414763.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
     gene            complement(11854..12603)
                     /gene="yafL"
                     /locus_tag="N7B39_003259"
     CDS             complement(11854..12603)
                     /gene="yafL"
                     /locus_tag="N7B39_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HGU2423188.1"
                     /translation="MSLPSIPSFVLSGLLLICLPFSSFASATTSHISFSYAARQRMQN
                     RARLLKQYQTHLKKQVSYIVEGNAESRRALRQHNREQIKQHPEWFPAPLKASDRRWQA
                     LAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDKGFDCSGLVFYAYNKILE
                     AKLPRTANEMYHYHRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRT
                     GETIRVSRLAEPFWQDHFLGARRILTEETIL"
BASE COUNT         3144 a         3249 c         3200 g         3096 t
ORIGIN      
        1 aaactttcct gttttcaacg gggctctccc gcagaatatt cgcgcgttaa ttaaaatcag
       61 gaatgaaaat gaaaaagagc actctggcat tagtggtgat gggcattgtg gcatctgcat
      121 ccgtacaggc cgcagaaata tataacaaag acggtaataa actggatgtc tatggcaaag
      181 ttaaagccat gcattatatg agtgataacg acagtaaaga tggcgaccag agttatatcc
      241 gttttggttt taaaggcgaa acacaaatta acgatcaact gactggttat ggtcgttggg
      301 aagcggagtt tgccggaaat aaagcggaga gtgatactgc acagcaaaaa acgcgtctcg
      361 cttttgccgg attgaagtat aaagatttgg gttctttcga ctatggccgt aacctgggcg
      421 cgttgtatga cgtggaagcc tggaccgata tgttcccgga atttggtggc gactcctcgg
      481 cgcagaccga caactttatg accaaacgcg ccagcggtct ggcgacgtat cggaacaccg
      541 acttcttcgg cgttatcgat ggcctgaact taaccctgca atatcaaggg aaaaacgaaa
      601 accgcgacgt taaaaagcaa aacggcgatg gcttcggcac gtcattgaca tatgactttg
      661 gcggcagcga tttcgccatt agtggggcct ataccaactc agatcgcacc aacgagcaga
      721 acctgcaaag ccgtggcaca ggcaagcgtg cagaagcttg ggctacaggt ctgaaatacg
      781 atgccaataa tatttatctg gcaacttttt attctgaaac acgcaaaatg acgccaataa
      841 ctggcggctt tgccaataag acacagaact ttgaagcggt cgctcaatac cagtttgact
      901 ttggtctgcg tccatcgctg ggttatgtct tatcgaaagg gaaagatatt gaaggtatcg
      961 gtgatgaaga tctggtcaat tatatcgatg tcggggctac atattatttc aacaaaaata
     1021 tgtcagcgtt tgttgattat aaaatcaacc aactggatag cgataacaaa ttgaatatta
     1081 ataatgatga tattgtcgcg gttggcatga cctatcagtt ttaatgatta ttgccggatg
     1141 tgatgcatcc ggcacatttc actcacgccg ttaacttcac cggctcgtca cgaaaatcat
     1201 ccgccggttc caacttcaga ttcagcgtcg tcagcagttc acgcagcttc tcgtgaaact
     1261 cacgcagggt gtgctccagt cgttcaacca cttcagtatc ttttaccgga acactcttcc
     1321 agtcgcctgc tttatcgaac agaccaaact ggtaactgta ggtaaaacgg gattcctgcg
     1381 cttcaagctc catccaccag ccccagaact cacgcacttc cggtgccggt ttcacgttga
     1441 cgcatacagc cagacaatcg aaaaagaatc gattatcttc gcacttacct tcacgaatat
     1501 acgggcctag tacggtaaat tttttgatca atctgctctt cgggtgtcca ctcggtaacg
     1561 tcattgctat ctcctgttgt gatgcaactg ttttaccaaa ttggcaaaat ttagcaaatt
     1621 tttaacacaa gcgtttttcg atccagtcgg tgatttcctg aagacctttg tcaaaattcc
     1681 gatacaccgg gttaaatggg atctctaata atttaccgtc agcagatgat gaggtgatta
     1741 agcgtgagtc ctcttccggg ctgaacggat cgttcttcca gtagcctgat aacattggcg
     1801 ttgggcatcg acgtcccagc aatccttgca cttttaatga atagcgattc agctccacgc
     1861 gcaacgcttc gtcggaagca tcatgcatcc ccaaacgact cgccagaacg tcaagataca
     1921 tttccggcac ctgttgctgg cacttaaaat cactcaacag ggtatgaact accggaccaa
     1981 gacaggcaac cgctttcaga cgcggcgatt caagatatgc cagacgcacg gcaacgttag
     2041 cgccgaaacg gaaaccaaag gccgcgacgc gagtgtgatc cacccacggt acgttaggca
     2101 gagcctttaa gacgcgctga tgcaacaggc aggagtcctg ggtgagcttc cattttgaag
     2161 aaaagcccac cgacggcata tcaatagtca gcatcgcaat gccgcgcggc gcaaaataac
     2221 gttcatacag gctgtaatag tccgtctgca tcgcatccag accaccacac attaataccg
     2281 tcgggaacgg gccatcgcct ttcggcatat gcaaaaagcc ggtgatgggc gcaccgccgg
     2341 gtacggtaaa ctccatctgt cgcatcgtgc ccggtagacg ctgagcggcc tcttcatagg
     2401 cgcggtttga caaagcctgc gcttgctcgg ccaggtcatc tcctttcaga tgaggatagg
     2461 cggcaatgtt gtacaacgta gccgcatgca gccagtgacg accactgagt tgcggatctt
     2521 cttccgcaca ggctttttgt tgccacacca tcgcctgggt ggcccactca taaatccagt
     2581 tgccgccacg gtagccaatc accgtatcgt ataaatcatc gtcggtacgt tcggcatcgc
     2641 tcatcacaat acgtgcctgg acgtcgagga tttcgcgtgg gtcaatgccg cgccagatcc
     2701 acatcagacg gttaatcatg cgataccagt gagggatggt tttaccatca agggccgact
     2761 gcacaggcgg ttgtgcgccg tgattaaagc ggcggactag cgtcgaggtt tcaggatgtt
     2821 taaagcgggg tttgaacagg gtttcgctca ggtttgcctg tgtcatggat gcagcctcca
     2881 gaatacttac tggaaactat tgtaactcgc ctgaagttaa aaagaacaac gcccggcagt
     2941 gccaggcgtt gaaaagatta gcgaccggag attggcggga cgaatacgac gcccatatcc
     3001 cacggctgtt cgatccaggt atcttgcggg atatcaacaa cataatcatc aaccagcgga
     3061 cgaccagccg gttttgcaaa gatggtgaca aagtgcgctt ttggatacat ttcacgaatc
     3121 gcaaccgcag taccaccggt atccaccagg tcatcaataa cgatgaagcc ttcgccatcg
     3181 ccttctgcgc gtttcagcac tttaagctcg cgctggttgt cgtgatcgta gctggaaata
     3241 caaacggtat cgacatgacg aatacccagt tcacgcgcca gtaacgcacc cggtaccaga
     3301 ccgccacggc ttacggcaat aatgcctttc cattgttcag aaggcatcag tcggcttgcg
     3361 agtttacgtg catggatctg caacatgtcc caggtgacga tgtatttttc gctcatgtga
     3421 agtgtcccag cctgtttatc tacggcttaa aaagtgttcg aggggaaaat aggttgcgcg
     3481 agattataga gatctggcgc actaaaaacc agtatttcac atgagtccgc gtctttttac
     3541 gcactgcctc tccctgacgc gggataaagt ggtattctca aacatatctc gcaagcctgt
     3601 cttgtgttga caacattttc tgctaaccct gtgacctgca atactgtttt gcgggtgatc
     3661 gacaaggaga cttaacgtgt ctgaactgtc tcaattatct ccacagccgc tgtgggatat
     3721 ttttgccaaa atctgttcta ttcctcaccc gtcctatcat gaagagcaac tcgctgaata
     3781 cattgttggt tgggcaaaag agaaaggttt ccatgtcgaa cgcgatcagg taggtaatat
     3841 cctgattcgt aaacctgcta ccgcaggtat ggaaaatcgt aaaccggtcg tcttgcaggc
     3901 ccacctcgat atggtgccgc agaaaaataa cgacaccgtg catgacttca cgaaagatcc
     3961 tatccagcca tatattgatg gcgaatgggt taaagcgcgc ggcaccacgc tgggtgcgga
     4021 taacggcatt ggtatggcct ctgcactggc ggttctggct gacgaaaacg tggttcacgg
     4081 cccgctggaa gtgctgctga ccatgaccga agaagccggt atggacggtg cgttcggctt
     4141 acagagcaac tggttgcagg ctgatattct gattaacacc gactccgaag aagaaggtga
     4201 aatctacatg ggttgcgcgg ggggtatcga cttcacctcc aacctgcatt tagatcgtga
     4261 agcggttcca gctggttttg aaaccttcaa gttaacctta aaaggtctga aaggcggtca
     4321 ctccggcggg gaaatccacg ttggcctggg taatgccaac aaactgctgg tgcgcttcct
     4381 ggcgggtcat gcagaagaac tggacctgcg tcttattgat ttcaacggcg gcacactgcg
     4441 taacgccatc ccgcgtgaag cctttgcgac cattgctgtc gcagctgata aagtcgacgc
     4501 cctgaaatct ctggtgaata cctatcagga gatcctgaaa aacgagctgg cagagaaaga
     4561 gaaaaatctg gccttgttgc tggactctgt agcgaacgat aaagctgccc tgattgcgaa
     4621 atctcgcgat acctttattc gtctgctgaa cgccaccccg aacggtgtga tccgcaactc
     4681 cgacgtggca aaaggtgtgg tcgaaacctc cctgaacgtc ggtgtggtga ccatgactga
     4741 caataacgta gaaattcact gtctgatccg ttctctgatc gacagcggta aagactacgt
     4801 ggtgagcatg ctggattcgc tgggtaaact ggctggcgcg aaaaccgaag cgaaaggcgc
     4861 atatcctggc tggcagccgg acgctaattc tccggtgatg catctggtac gtgaaaccta
     4921 tcagcgcctg ttcaacaaga cgccgaacat ccagattatc cacgcgggcc tggaatgtgg
     4981 tctgttcaaa aaaccgtatc cggaaatgga catggtttct atcgggccaa caatcaccgg
     5041 tccacactct ccggatgagc aagttcacat cgaaagcgta ggtcattact ggacactgct
     5101 gactgaactg ctgaaagaaa ttccggcgaa gtaattattt gatttgctgc cggatggcgt
     5161 ttaatcgcct tccggcagtt tcatccttca ttatccttcg ataaaagcca tccctgtgaa
     5221 cgttcgtcgc gggttgccac gttcaatctg gtgatggaac attcgccgct gcgatttcag
     5281 cactgcgcta ttttcctgtt gctgctgctc cagcttccag gcaatcagca atcgcgccag
     5341 ccgtttgtta gcatgctggc tgcgttctga ctgaaccttc acgctaatcc cggttgccag
     5401 atgcgtggcg cgtaccgccg agtcggtttt attgacatgt tgaccgcccg gccccgacgg
     5461 acgcagcgtc tcataacgga ttgcatccga ttgttcctgc tcatcagcgg taaaacgccc
     5521 aatgcccaga aaccagtttt tgcgcccatg atgaggccga tacggactca gacaaatcca
     5581 ctgaatagtg ccgcaccaac tttcgcttaa cgcccatgcg ttgtcgccat ccagagaaat
     5641 cagcgccgaa cgcagcgtgt cagagtagcg gcccgtttct gtttccagca ccgttaccgc
     5701 gacgtcttgt cgggtaaaaa cgaccaacag agaagaacgc tgcgccaatc gggtagccgc
     5761 gtccggggtg taaatctggc atccccacta cgcgctgcat gttgggtaaa tttgccgtgg
     5821 tgtgtaattg ttggatcgct aaactctcga tccacaggtc atcagatgcg atggtaaata
     5881 ccgcatcgga taagggacga atatatttgc ccatgtacca ttccatttat actcgtcata
     5941 cttcaagttg catgtactgc gactgcgttc gttcacccca gtcacttact tatgtaagct
     6001 cctggggatt cactcgcttg tcgccttcct gcaactcgaa ttatttagag tatataaata
     6061 taaaaatcag gaaatggcag acgaatccag ggcaagaaaa aaaagaatta atcctggaaa
     6121 ggtctgcaaa gagtcattga taaaggattt gtcatgattt acctccttgc agtttgtgga
     6181 ataagaaaac gcgcacatac caggcgggcg ttattttcat tgcaagctgg atttaatgtt
     6241 gcggtttata tcgcatagaa aaattagtaa accacgctcc ccggcattca acgcgctgct
     6301 gcttaactgt ctgaaaacca taacgttcaa aaaagggttt tgcggttatg ctggcgtcca
     6361 ccgtaagttc ggattcagac ttaatccaag gttttaacaa agcgctggca accccacggc
     6421 gggtgtattc agggtcaaca aataacatat cgatataatg ttcaatgcgg gaaataaaac
     6481 caaccggttg tgcattaatg actgcaaccc gcacttgtga tttcgcgagt ttctccttcc
     6541 agcgagattc gtcaatctgc gcccaggcgg caatttgttg tggtgaataa tgctgactgg
     6601 cggtcatcat aaccgctcta atgaaaatag cgcatagttg ctgaaaatcg ccaggctgat
     6661 agtttcttat ttgtatgtta ttcataatat aaattcaaaa acgcatgcga aacgcttctg
     6721 ccattttccc aattttatgc atttggttgt tatctcgagc cagtttatga ggttcaggtt
     6781 tcagaatggc aatgagcaac catgcttgct catcaaacgc accctgacaa tataccaaat
     6841 gcgattcgtc attcgttctg ctgaattggc gcaactgtgg cggaaatgga ttattcacat
     6901 ttgccagatg aatatgagca actcgctcaa atttgattaa tggccaagta aacgagtcgt
     6961 cgtagagtgc atcgcgacca aatatatctg gcaaaacacc gtcacgctta taggaaataa
     7021 aatccgccgt taacgcatca agctcctctg ctgtaagttg caggcgaata agttttgttt
     7081 tgaatacccg catccttatt ccttaaagtc attgaaatca tcatccgtca tatcattaag
     7141 atatttttca gctcggcgtg taatttcctc taatcttgca gcacttggac ggaagcgcgc
     7201 ctttatgggc tttttctcct cttgttcagc aagcatgtcc attaacgctt cgaatgcgct
     7261 ggcacttaag agatatcctg cggggcgatt attagaaaga accgcaaccg gttgatcaat
     7321 aaagtattta gctgggtttt tacgtaattc agtgatattg accgattttt cagcgagaat
     7381 tcgatgcata cagtgatccc ctcataataa tgcacaccag aatatacata atagtataca
     7441 tcataatccc agcaccagtt gtctttccat ttgagggtca agcaacgtca catgcagccc
     7501 caccagtcgc acaccgcgcc cgccgcggcg ttcatcccag gttttacgcg cggtggcgat
     7561 taagtcagct ttattcagcc gcggccagac gtgctcctgg gtggtttgct gaaaatcgtc
     7621 gaactttaat ttcaccccct ggcgagcaat cagtaaatca ggttttacct ttgccagacg
     7681 gcgttcaagt tccggataca gccgctcgat aatcgcttca cattcagacc agtgatgaat
     7741 atcttccgcc atcgtgcgtt ccacgccgac ggattttcgt aaccgttcgc tgttaacgtc
     7801 gcgctcgtca atcccctgac tacgctccca caaaatgcgg ccaaatttgc caaagcgttt
     7861 aagcagaatc accagatcac acttttgtac atcaccgcag gtccgcagcc ccatcgcttc
     7921 cagctttgcc gctgagactt tgccaacacc gggaattttt gccagcggta aggtttgcag
     7981 aaatgccgaa acttctgccg gcgtaatcac aaactggccg ttgggtttat tcatgtcgga
     8041 ggcgattttg gcgagaaact ttactggtgc cacgcccgca gacgccgtca gttgcagctc
     8101 gctgaaaatt gtctggcgga tttcctgggc gatgagggtc gcagaaccgt ggcaatggac
     8161 gctatcagtg acatcgaggt aggcttcatc cagtgacaac ggttcaatac gcgaggtgta
     8221 gcgcgagaag atttcacgga tatgatttga ggcttctttg taggcgtcaa agcgccccgg
     8281 aagcaaggtg agatgtgggc ataatttgag cgccatccct gtcggcatag cgctgcgtac
     8341 gccaaattta cgcgcgggat aattggcggt gctgatcacc ccccgacgtt cgcggctgcc
     8401 gccaatagca atagggatat cgcgcagggc gggattgtcg cgcatctcca ctgcggcgaa
     8461 aaagcagtcc atatccacat gaatgatttt acgcattact cacctctcaa tactggtaaa
     8521 gtatacagtg atttcagggt ttgagaaatg cgtaaagatt cagcatttag cgcgttcgct
     8581 gcgaaagcac ctgctgctta tccagctttt gcaccagcgg ctgcaccact ttatccccat
     8641 gctgaccaaa gtattgatac agcgtatcgg acgcactttt ggtcagcacc ataatctcaa
     8701 tgcgtcggtt gcccgcgctt tgcggatttt tcgcgtccag cagcatctgg tccgccattg
     8761 cgcttacctg catcacttta tcttccggca ttccggcctc ttccagcacc cgacgagctg
     8821 aaagcgcgcg atcgcccgaa aggttccagt tgttgtagat attgtttttg taggccatcg
     8881 catcggtatg cccggtaata ataattttgt tatcgagcga gtcgaacact ggtgcgagct
     8941 ccaccagcaa agttttaaag aacggcataa tctgcgcgct gccgcgttcg aacatattgc
     9001 gattctggtc gtctttaatc agcacgcgta atccctgcgg gacaatctcc atctccagat
     9061 tcgcttccat atgcgcatca cgggcaatgg tgttgatact ggttgccagc tcgccaagct
     9121 cggtggcgga tttcttcttc agcagcgccg ttttctcatt cacatcacgc gcttttttct
     9181 ccgtctcttc cggcaccgcc acggtggcag gttttggcgg atgagacggg ctgattttat
     9241 ttaacggcga cagcccacca ccgttaaaaa tcgactgccc gtgcagcgcg gcgataatgc
     9301 tttcacgctc ggatttactg acgctgttga caatcaactt tatcgtcgtc accaaagggg
     9361 ccgagcgtat ttccgaggtt tctgcccgct ttaccctgga tgcgatgccc ggcaaacaga
     9421 tggcgattga cgccgatctt aacgccggat tgatcaacca ggcacaggcg caaacccggc
     9481 gtaaagatgt tgccagcgag gccgatttct acggcgcgat ggacggggca tcgaagtttg
     9541 tgcgcgggga tgccatcgcc gggatgatga ttctggcgat taacctgatc ggcggcgtct
     9601 gtatcgggat atttaaatac aacctgagcg ccgacgccgc cttccagcaa tatgtgctga
     9661 tgaccatcgg cgatgggctg gtggcgcaga tcccttccct cctgctctcc accgcagcgg
     9721 cgattatcgt cacccgcgtc agcgacaacg gcgatatcgc ccatgacgtg cgccaccagc
     9781 tgctggcaag cccgtcggtg ctctacaccg ccaccgggat tatgtttgtg ctggcggtgg
     9841 tgccgggaat gccgcacctg ccgtttttgc tgttcagcgc cctgctgggt tttaccggct
     9901 ggcggatgag caaacagccg caggcggcgg aggcggaaga gaaaagcctc gaaacgctga
     9961 cccgcaccat cactgaaacc agcgagcaac aggtcagttg ggaaaccatt ccgcttatcg
    10021 agcctatcag tttaagcctc ggctacaagc tggtggcgct ggtcgataaa gcgcagggca
    10081 acccgctcac ccagcgtatt cgcggcgtac ggcaggtgat atccgacggt aacggcgtgc
    10141 tgctgccgga gatccgcatt cgggaaaact tccgcctcaa gcccagccag tacgccattt
    10201 tcatcaacgg cattaaggcc gatgaagcgg atattccggc ggataaactg atggcgctgc
    10261 cctccagcga aacctacggc gagattgacg gcgtgctggg gaacgacccg gcgtacggga
    10321 tgccggtgac ctggatccag cccgcgcaga aggcgaaggc gctgaatatg gggtatcagg
    10381 tgatcgacag cgccagcgtg attgccacgc atgtgaacaa gattgtgcgc agctatattc
    10441 ctgatttgtt taattatgat gacatcacgc agctgcataa ccgcctgtcg tcgatggccc
    10501 cgcgcctggc ggaagatttg agcgcggcgc tgaattacag ccagttgctg aaagtgtacc
    10561 gtgcgctgct gaccgaaggc gtttccctgc gcgatatcgt caccatcgcc accgtgctgg
    10621 tcgccagtag cgcggtgact aaagatcata ttctgctggc ggccgatgtg cgcctggcgc
    10681 tgcggcgcag cattacccat ccgttcgttc gcaagcagga gctgacggtg tatacgctga
    10741 ataatgagct ggaaaatctg ctgactaacg tagtgaatca ggcgcaacag ggcggtaaag
    10801 tgatgctcga cagcgtgcca gtggacccga atatgctcaa ccagttccag agcacgatgc
    10861 cgcaggtgaa agagcagatg aaagcggcgg ggaaagaccc ggtgctgctg gtgccgccgc
    10921 agctgcgccc tttgctggcg cgctatgcaa ggttgtttgc gccggggctg catgtgctgt
    10981 cgtataacga agtgccggat gagctggagt tgaagataat gggggcgttg agttgattgg
    11041 cctttaaggg gcgcatccgg cattggtgca caattgccgg atgcggcgtg aacgccttat
    11101 ccggcctacg gttgagtgca gttttgtagg catgataaga cgcgccagcg tcgcatcagg
    11161 catccggcac cagcgcacaa ttatgaaatg atacgctccc cggcactaaa atccgttatg
    11221 tctcccgccc aatcaatggg atataaccct cgcgccacat cgcgatggaa cgttgagaat
    11281 ggccaatcac tcacttgctt tacccagcca tgttttactg gatttatata aatataatca
    11341 acatgatggc gatagtcttt ggtattgcgg atggtgtgct cctaaaaacg tggttgccag
    11401 atatttttca atccacaagc atgggtgaac tgctttttaa tctcccgcca gcgggaggaa
    11461 aaatcatcat cgctttcggg taatgtccat atgcaatgca tatgctccgg caaaacgacc
    11521 caggcgttaa tttcaaaagg tctgtctcgc ttaacgttaa taatggcgtt acggagcatc
    11581 tggaactgag cggtcagaag gtggcttcga cgatttcgta ggttaaccgt gaaaaaccag
    11641 gttccccctt tgatgtaata ccgacgatat tcagacatgg caactccttt ttctcaacgg
    11701 taagaagaaa aggaaaggtc ggcaatgagg tgatggaata acgggaaagc ggctcgcaaa
    11761 aacgcacaga tttgtaggca tgataagacg cgtcagcgtc gtatcaggca tctgcgcaca
    11821 actgccggat gcggcgtgaa tgccttatcc gtcctacaaa atcgtctctt ccgtcaaaat
    11881 ccggcgcgcg cctaaaaagt gatcctgcca gaaaggttca gctaaccgac ttacccgaat
    11941 ggtttccccg gtgcgcggtg actcaataaa ctgaccgtcg cccagataca cccccatatg
    12001 gtcggcaatc tcgcggctgt ggatatggaa aaacagcaaa tctccccggc gcaggtcgtt
    12061 attcgccaca atcgttgccc gatggtagtg atacatctca ttagccgtac gcgggagctt
    12121 cgcctcgagg atcttgttgt aggcataaaa aaccaacccg ctacagtcaa agcctttatc
    12181 aggccgcgtg ccgccccaga cgtaaggctt gccaagctgc tgctccaggc ggtgaatcgc
    12241 cacttcggta atgttatgca gatggtcgct gcttaaaaag tggttgtttt ccgccagcgc
    12301 ctgccagcgt ctgtcactcg ccttgagcgg tgcaggaaac cattctggat gctgttttat
    12361 ctgttcgcgg ttgtgctggc gtagcgccct tctgctttcg gcattgcctt ccacaatata
    12421 gctgacctgc tttttcagat gggtttggta ctgttttaat aaacgcgcac ggttttgcat
    12481 ccgctggcgg gcggcgtagc tgaaagagat atgtgatgtg gtggcgctgg caaatgaaga
    12541 aaacggcaaa caaatcaaca gtaatcccga caatacaaag gatggaatcg acggcaagga
    12601 catactcaat aatggctggt catgcattga tgttacagtg tatcccggcc cgtacctgaa
    12661 caaacttaat aaaccattat tataaaatt
//
LOCUS       DBFIFI010000124        12579 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.124, whole genome shotgun sequence.
ACCESSION   DBFIFI010000124 DBFIFI010000000
VERSION     DBFIFI010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12579
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.124"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(36..944)
                     /gene="galU"
                     /locus_tag="N7B39_003260"
     CDS             complement(36..944)
                     /gene="galU"
                     /locus_tag="N7B39_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HGU2423189.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(1146..2159)
                     /gene="rssB"
                     /locus_tag="N7B39_003261"
     CDS             complement(1146..2159)
                     /gene="rssB"
                     /locus_tag="N7B39_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HGU2423190.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(2251..3156)
                     /gene="rssA"
                     /locus_tag="N7B39_003262"
     CDS             complement(2251..3156)
                     /gene="rssA"
                     /locus_tag="N7B39_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HGU2423191.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            3269..3727
                     /locus_tag="N7B39_003263"
     CDS             3269..3727
                     /locus_tag="N7B39_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HGU2423192.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELIAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            3777..4619
                     /gene="purU"
                     /locus_tag="N7B39_003264"
     CDS             3777..4619
                     /gene="purU"
                     /locus_tag="N7B39_003264"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HGU2423193.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVSRFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            4779..4863
                     /locus_tag="N7B39_003265"
     tRNA            4779..4863
                     /locus_tag="N7B39_003265"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4813..4815,aa:Tyr,seq:gta)
     gene            4908..5038
                     /locus_tag="N7B39_003266"
     ncRNA           4908..5038
                     /ncRNA_class="other"
                     /locus_tag="N7B39_003266"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            5073..5157
                     /locus_tag="N7B39_003267"
     tRNA            5073..5157
                     /locus_tag="N7B39_003267"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5107..5109,aa:Tyr,seq:gta)
     gene            5204..5335
                     /locus_tag="N7B39_003268"
     ncRNA           5204..5335
                     /ncRNA_class="other"
                     /locus_tag="N7B39_003268"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            5382..5513
                     /locus_tag="N7B39_003269"
     ncRNA           5382..5513
                     /ncRNA_class="other"
                     /locus_tag="N7B39_003269"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(5697..6374)
                     /gene="narI"
                     /locus_tag="N7B39_003270"
     CDS             complement(5697..6374)
                     /gene="narI"
                     /locus_tag="N7B39_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HGU2423194.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(6374..7084)
                     /gene="narJ"
                     /locus_tag="N7B39_003271"
     CDS             complement(6374..7084)
                     /gene="narJ"
                     /locus_tag="N7B39_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HGU2423195.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLPKEDAHALG
                     IFLRDLTTMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLAQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVLFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(7081..8619)
                     /gene="narH"
                     /locus_tag="N7B39_003272"
     CDS             complement(7081..8619)
                     /gene="narH"
                     /locus_tag="N7B39_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HGU2423196.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(8616..12359)
                     /locus_tag="N7B39_003273"
     CDS             complement(8616..12359)
                     /locus_tag="N7B39_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HGU2423197.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
BASE COUNT         3000 a         3415 c         3102 g         3062 t
ORIGIN      
        1 tcaacgccgt ttcgtggata acaccgatac ggatgttact tcttaatgcc catctcttct
       61 tcaagccagg ctttaaattc cgtgccaagg gtgttatgac gaataccgta ttcaacgaag
      121 gcctgcatgt aacctaattt attaccgcag tcatggctct tccctttcat atgataggct
      181 tcaaccgttt ctttttcgat tagcatatca attgcgtcgg tgagctgaat ttcatcacca
      241 gctcccggag gggtttttgc cagcaacggc caaatatccg cactaagtac gtaacgaccc
      301 acaatagcga gattagacgg cgcaacatcc gcttttggtt tttcaaccac gccaaccatc
      361 ggtacgcttt cacccggcgc taattcaacg cctttgcaat ccacaacgcc atatgcggtc
      421 acatcagcaa ccggttcaac catgatctgg ctatgacccg tttcatcgaa gcggcggatc
      481 atctctgcca ggttatcctg tgacaaatcg gattcatatt catccagaat aacgtcaggc
      541 aaaataacag ccacaggttc atcacccact accgggtgag cacacaatac cgcgtgtccc
      601 aggcctttcg ccagcccctg acgaacttgc ataatagtta cgtgcggcgg acaaatagac
      661 tgcacttcat caagcagttg acgttttaca cgtttttcca gcattgcttc cagttcaaaa
      721 ctggtatcaa agtggttttc aatagagttt ttagatgagt gtgtaaccag cacaatttca
      781 gtaatgccag ccgcaataca ttcattcacg acgtattgaa ttaatggctt atcgacaagt
      841 ggcagcatct ctttcgggat ggctttcgtc gccggcaaca tcctggttcc taatcccgca
      901 acggggataa cggctttttt gactttcgta ttaatggcag ccatttaaat tctcctggac
      961 tgttcgtgtt ttgaacgtgt tcatatttct gtatcgcatc ccagtatatc agcaccccgg
     1021 aggggctaag ttctgaaaag gatgcgagtt agcataatta agacaattac ttatacgaat
     1081 ggcgacgata gtaccaccag ccgacattag ctggtaatgc aaatttagcc cgcgttatcg
     1141 tttgctcatt ctgcagacaa catcaagcgc agtcgaccac cggttcccca tatttggcat
     1201 tgccaggcat cgcatcgctg gctcaattga ttcaaataag cgttacctaa agtgcctaac
     1261 ggaacaccat tactgatttg cacctggtgt tcgccggtat ttaacgtcgc attcagaccc
     1321 gcagaaacca gaatgagatt tttcagttcg cgatgataat agccaactaa tagcggaaac
     1381 tgccccggca gattggcctg acggagtaaa tggtttacct gcttcaataa cgcgcccaac
     1441 tctggcaacc gttgattttg gtgtgcaagc tgttcctgta ataatccgtt aaacaatgcg
     1501 cgtaataaca aggcagcaag tacgccatta tgtccagctc gggttacatc aaggcaataa
     1561 aatgccagat cgttttccga aagtgcggca atatcaagca ccaggccggg tttgtccgcg
     1621 gcaaccaatt gacggtaatt aacccggcaa tgggaaatca cctgctgaac cggcggttgt
     1681 agttcctgta ataatttcgc cgctgcggca gggttatcaa ccattgcatc ccagtcgcga
     1741 aaaagccttt cctcttcctc aacgcgcgaa ttaaacatgc tgggatagag acaggcaaaa
     1801 accatctcgc gcaagcgatt cagatcttta actggtttca gcaaaacatc ttcaacgccc
     1861 agacgtaacg ctttggcaat atctgccata ttttcagtgg cagatatcac cagaactggg
     1921 gtctggtcgc ctctgttacg tatatgctcc agcagtttaa gcccgttcat tcgtggcatc
     1981 gcgatatcac atatcatcag gtctggagtg aaacctccca gcaactcaag ggcatccacc
     2041 ccatcagccg ccagtaccgt tgtcgctccc aatgaggaaa accacgaatc cagaagcgag
     2101 cgaaatacct gctcatcttc aacaatgaga atctgttttc cgaccaatgg ctgcgtcatg
     2161 ttctctcccc tgactggctt tactcaatag tggcatgcta ttgccacctg cgcctgtcag
     2221 aattaactta agtgtagata aaaattctgg tcaaatgttg gtgcgtacca acggcaaaag
     2281 ttcgtccatt ttcttttcca ctgccagctg cccggccgca atggcagcgt gcgcgcgatg
     2341 gaaatcaagc gtagatattt gcgggcaaac aggttgaatc agaatatcgg gcggatcacc
     2401 tgccatgcgg ttccttttaa ggcggttctc cagcacctgg atagaagtgg tcataatctc
     2461 tgttgccgtt ggtgctgtca ccgcccgacg tgtcgttata ctgcctaacc tttctttcag
     2521 acgcgcatgc cacggcagag aatcaccatt ctcgctattt tcttcactga cattaaagga
     2581 gagcaaatct tgttgcatca aatgagcatc gtgctgcagg tcgaccgcta tcacaatatc
     2641 agcacccaat gcacgcgtga gcgaaatagg aattgggtta acgactgccc catcaaccag
     2701 ccagtagccg ttatgcgcaa cgggtgccat tagtcctgga atactgcatg atgcgcgaat
     2761 agcaagatgg agatcgcctt cagtaaacca taattcacgc ccagtactta aattggtggc
     2821 aacagccgcg aagcgacggg aacaattttc gatctctgtt tccggcatta tttcgcggta
     2881 ttgattgaag acacgctcgc cgcgcagtaa cccaccgcgc tgccaggaga gatccatcag
     2941 tcgtaaaaca tcccaataac tgaaagaggt cacccaatct tccagcgcag ataatcgatc
     3001 gcatgcatag gccgcgccca ccagcgaacc aattgaacat cctgcaacga tatcaatttc
     3061 aatacccact ttttttagcg cattaataac gccaatatgc gaccaacctc tcgccgcgcc
     3121 agatcccagc gccagcccta tttttatctt tctcattatt cccgcaagat tcccctggaa
     3181 tgcaatcgta gccacatcgc tactgctcgg ttaacatagt gctactcagg cggttaatac
     3241 gccgttattg tttccaggga gattatttgt gtctcagctt tgtccctgtg gtagtgctgt
     3301 cgagtatagc ctatgttgcc acccttatgt gtctggtgaa aaggttgcac ctgatccaga
     3361 acatctcatg cgttcgcgtt actgcgcttt tgtgatgcaa gacgcagatt atttaataaa
     3421 gacctggcat ccatcttgtg gagcagcggc gttacgtgcc gaattgattg ccggatttgc
     3481 gcatactgaa tggttgggat tgaccgtatt tgaacattgt tggcaggacg ccgacaatat
     3541 cggatttgtc agttttgttg ctcgatttac cgaaggtggc aaaacaggtg caataattga
     3601 acgttcgcga tttttgaaag aaaacggtca atggtactat attgacggta cacgtccgca
     3661 gtttggccgc aacgatccct gcccttgtgg ttcaggtaaa aaatttaaaa agtgctgcgg
     3721 ccaataatgg ttgacggtac ggtttagcaa acactctcaa caaggttttt ccagcaatgc
     3781 attcactcca acgtaaagtt ctgcgtacta tttgtccgga ccaaaaaggt ctgatcgcac
     3841 gtattaccaa tatttgctac aagcacgagt taaatatcgt acagaacaat gaatttgttg
     3901 atcaccgtac cgggcgcttt tttatgcgca cggaactgga agggattttt aatgattcca
     3961 ccctgctggc ggatctcgat agcgcattgc cagaaggctc cgtgcgtgag ctgaatcctg
     4021 ccggtcgtcg ccggatagtg attctggtca ctaaagaagc gcattgcctt ggcgatttgt
     4081 tgatgaaagc caattatggc ggcctggatg tcgaaatcgc ggcagtgatt ggtaaccacg
     4141 atactttacg ttctctggtt gagcgttttg atataccgtt tgagctggta agccatgaag
     4201 ggttaagccg caacgagcac gatcaaaaga tggcggatgc cattgatgct tatcaacctg
     4261 actacgtggt gctggcgaag tatatgcggg tattaacacc ggaatttgtg tcacgcttcc
     4321 cgaataagat catcaatatt caccattcct tcctgccagc gtttatcggc gcacgtcctt
     4381 atcaccaggc ctatgaacgt ggcgtgaaga ttattggcgc aaccgctcac tatgtgaatg
     4441 acaatctgga cgaaggccca atcatcatgc aggacgttat tcatgtcgat catacctaca
     4501 cagctgaaga tatgatgcgc gcaggtcgtg acgtcgagaa aaacgtctta agtcgcgcgc
     4561 tctacaaagt actggcgcag cgcgtctttg tttacggtaa tcggacgatt attctttaat
     4621 cgccagcaaa aataactggt tacctttaat ccgttacgga tgaaaattac gcaaccagtt
     4681 catttttctc aacgtaacac tttacagcgg cgcgtcattt gatatgatgc gccccgcttc
     4741 ccgataaggg agcaggccag taaaaagcat taccccgtgg tggggttccc gagcggccaa
     4801 agggagcaga ctgtaaatct gccgtcatcg acttcgaagg ttcgaatcct tcccccacca
     4861 ccatgattca ccacagcgat gtggtaactt cggtgagatt gtgaaagttt gcactcatcg
     4921 gggaagggtg agaaccttcg actaaggttc gactcgagcg aaagcgagag aacgttttca
     4981 caggcaacgg cccgaagggt gaggagcgaa gcgacgaata atcctttccc caccgccatc
     5041 tcaagcatta cctcaaaatt tgaattaccc ctggtggggt tcccgagcgg ccaaagggag
     5101 cagactgtaa atctgccgtc atcgacttcg aaggttcgaa tccttccccc accaccatca
     5161 ctttcaaaag tccctgaact ctcaagcgaa tccgtaaata aatattctgc ccaagcggag
     5221 aaggatgaga agcttcgact aaggttcgac tcgagcgcca gcgagagagc gttgccgcag
     5281 gcaacgacct gaagggcgaa gcgcgcagcg ctgagtaatc cttcccccac caccatcact
     5341 ttcaaaagtc cctgaactct caaacgaatc cgtaattaaa tattctgccc aagcggggaa
     5401 ggatgagaag cttcgaccaa ggttcgactt gagcgccagc gagagagcgt tgccgcaggc
     5461 aacgacccga agggcgaagc gcgcagcgct gagtaatcct tcccccacca ccatcgcttc
     5521 ctgaaataat caaaacctgt tactccgagc taattttgcc caatggcacc acgtttaccg
     5581 gacctacaat atcctccctt gtaggtcgga taagcgaagc gcatccgaca actcaccccg
     5641 caccgtgccc ataaaaaaac cccgccgaag cagggtctat gtgaactaaa attcgcttag
     5701 tgacgagcgc gcaccagctg gtacttacgt gtcagatact ccaccggtac gctccagatg
     5761 tgtaccagac gcgagaacgg gaacagcagg aataacgtca tccccagcac caggtgtaga
     5821 cggaagataa acgccacacc atcgaggtgt tgagaagcgc caccgtggaa ggtcactacc
     5881 gactgcgccc agccaaccag tttcatcatc tcgctaccgt ccatatgttg ggcggagaac
     5941 ggaatggtca acaggcccag cgcgcactgg ataacgagca gcgacaggat cagtatatcc
     6001 gctccggtag tcgttgcacg cacgcgtggg ctgaacagac gacgtttaag caaaagcacg
     6061 ccgccaatca gacacagcac gccgctggca ccaccagcaa acatcgccat tttctgtttc
     6121 acttcaatcg gcagccaggc ttcatacatc cagtgcggcg tcaacatacc gaagaagtga
     6181 ccgacaaaaa tccccagaat accgatatgg aacaggttcg acgccaggtt catccctttg
     6241 cgatccagca tctggctgga cgccgcgcgc caggtgtact gcccgtagtc ataacgcagc
     6301 cagctaccaa tcaggaagac cgccccggct atgtacgggt agatatcaaa gaagaacata
     6361 ttcaggaatt gcattagtgc tgtcctccgg tggtgatatt cagatattgc ggcgcgacgg
     6421 caccggcaaa gcgacgctga tgagcagtga ttgctgaatc accgcagcct ttgtcagcaa
     6481 agaatttaac ctgctcttct tcccaaacag catccagcgc ctgcggcgta tcatcgcgcg
     6541 cttcgtcggc aattttttcc gccactttgt cgctgtcgat agcggtattg gccaatttca
     6601 gcagcagatc aaacagcacg gcataacggc tttcacgctg ttgcagacgc gcactcagca
     6661 atgccagaat cggcgcgata tctttcaaac cttccacggc ttcgctttgc ggcagctgcg
     6721 ccaggtactc cagatacagc ggcagatgat ccggcaattc gcggctgttt aactgcaagc
     6781 cgtgctgctc gtactgcgcc agcaggtcca ccatcgcctg gccgcggtcg cgggattcgc
     6841 cgtgcacatg ttcaaacagc aacagtgacg tggcgcggcc acggtcgaac agttcgctgt
     6901 actgcgcctg ggcatcgagc ggatccatcg tcgttaaatc gcgcaggaaa atgcccagcg
     6961 catgggcatc ctcttttggc agatttttcg acgcggcaat cgcctcaaac atctcttgtt
     7021 gatgctgcca taaggcagca tccggatatt caaggagacg cgatacaatc acgagttcga
     7081 tcatggatgc ggctccgttt tgctggtcac atcgatggca tcgatacgac ggctgttgaa
     7141 cagattgaat ttggtatctg aaccgtggca gccatcacca aaggtaaagc cgcagccatt
     7201 tttctccggg aaggcttccc gtgccagttc acgatgacta ctcggcacca caaagcgatc
     7261 ttcgtagtta gcaatcgcca gataacggta catctcctgt gcctgggctt cggtcagacc
     7321 gacctcttcc agcgcacggg tatcaacttt accgtcaacg gtttcagcac gtttgtaatg
     7381 acgcatcgcc agcatacgtt tcagcgcgcg cagtaccggt ttggtatcac cggcggtcag
     7441 cagattcgcc agatactgta ccggaatacg caggctttcg acgtctggca gaatgccgtt
     7501 gctacccagc tcacccgcgt ctgctgcaga ctgaatcgga gagagaggcg gcacgtacca
     7561 gaccatcggc agtgtgcgat attctggatg cagcggcagc gccagtttcc attccattgc
     7621 cattttataa accggcgact gctgtgcagc ttcaataacg ctcagcggaa taccgtcttt
     7681 aatcgcctgc tcgatgactt tcggatcgtt cggatcgagg aacacctcca gctgacgctg
     7741 gtagagatct ttctcgttct cggtgctggc tgcacgttca atcgcgtcgg catcgtacag
     7801 cagcacgcca agataacgga tacgaccgac acaggtttct gagcacacgg tcggctgacc
     7861 cgcttcaata cgcggatagc agaagatgca cttctcagac ttaccgctct tccagttgaa
     7921 gtagattttt ttgtacgggc atccggtgat gcacatacgc cagccacggc atttatcctg
     7981 gtcgatcagg acgatgccat cttcttcacg cttgtaaatc gcaccgctcg ggcaggtcgc
     8041 cacacatgcc gggttcaggc agtgttcgca caggcgcggc aaatacatca tgaaggtgtt
     8101 ttcgaactgg ctatacatcg ccttctggat gttgtcgaag ttcttgtctt tcgccagttt
     8161 gtcaaactca ccgcccaggt catcttccca gttcggccct ttttcgattt tcgccatccg
     8221 ttccccggta atcagcgaac gcggacgggc aatcggctgc gatttgctgc cttccggcgc
     8281 ggtatgcagg ttctgatagt caaaatcgaa cggctcgtaa taatcgtcga tccccggcag
     8341 atgcgggtta gcgaagattt tacccagcag catggcacgg ttacccatgc gcggttgcag
     8401 tttgccgttg attttacgga tccagccgcc tttgtatttt tcctggtttt cccagtcagt
     8461 cgggaagccc tggcccggct tggtttccac gttgttgaac cacgcgtatt ccacgccttc
     8521 acggctggtc cagacgtttt tacaggtaac tgaacaggtg tggcagccga tgcacttatc
     8581 gagattcagc accatgccga cttgtgaacg aattttcatt ttacgctctc ctgtacctga
     8641 tcattgcctt cgccatctaa ccagtcaatg ttcttcatct tacgcactac aacaaactca
     8701 tcgcggttag aacctacggt gccgtagtag ttaaagccgt atgccagatg ggcatagccg
     8761 ccgatcatat gcgtcggttt cggcgtgata cgggtgaccg agttatggat accaccacgc
     8821 tgttgggtaa tttccgaacc aggcaggtta acgatacgtt cctgcgcgtg gtacatcatg
     8881 gtcatccctg ccggaacacg ctggctgaca accgcacggg cagtcagagc accgttgctg
     8941 ttgaatactt caatccagtc gttatcggcg atacccagat ctttggcatc ggcttcactc
     9001 aaccagacca ccggaccacc gcgacctaaa gtcagcatca gcaggttgtc gctgtaggtg
     9061 gagtggatac cccacttctg gtgcggcgtc aggaagttga gcgctttttc cgggttgccg
     9121 ttggatttct ggcctatcac ttctttcacc gaacgggtgt cgatcggcgg acgataaacc
     9181 agcaggcttt caccgaaatc acgcatccac tggtgatcct gatacagttg ctgacgacca
     9241 gagagcgtac gccatgggat cagctcgtga acgttggtgt aaccggcgtt gtaagaaacg
     9301 tgttcatctt ccagaccaga ccaggtcggg ctggagataa ttttgcgcgg ctgtgcctga
     9361 atatcgcgga agcggatctt ctcgtcttct ttattcagcg ccagatgcgt atggtcacga
     9421 ccggtaaatt cgctcagggc agcccaggct ttcacggcta cctgaccgtt ggtttccggt
     9481 gccagtgtca ggatcatctc tgccgcatca attgcggtgt tcagcatcgg ctggcctttc
     9541 gccggacctt ccgctttggt gtagttgagc ttacgcagca gatccatctc gctctgggtg
     9601 ttccaggcaa tccctttacc gccgttaccg attttctcca tcagcgggcc gatagaggta
     9661 aagcgttcgt aagttgccgg ataatcgcgc tctacgacca taatgtgcgg tgcagttttg
     9721 cctgggatca ggtcgcattc gccttttttc cagtctttca catccagcgg ctgcgccagt
     9781 tcagcggcag agtcgtgctg gataggcagc gtgacgatgt cggtttcttt acccagatgg
     9841 ccgacgcaca cttcggagaa tttcttcgcg atggctttgt agatttccca gtcgcttttc
     9901 gcttcccagg ccggatcgac cgccgcagac agcgggtgaa taaacggatg catatccgaa
     9961 gtattcatgt cgtctttttc gtaccaggtc gccgtcggca aaatgatgtc ggaatagaga
    10021 caggtgctcg acagacggaa gtccagcgta accaccagat ccagcttgcc ttccagacca
    10081 ttgtcctgcc agtccacttc ttccggcttc acgccgccct gctgccccag atctttaccc
    10141 tggataccgt gctccgtccc cagcaggtac ttgagcataa actcatgacc tttaccggaa
    10201 gaaccgagca ggttagaacg ccagataaac aggttacgcg ggtggttttt gccgttttcc
    10261 ggctgttccg cagcgaaacg gatagaaccc tctttcaggg atttcaccgt atagtccacc
    10321 ggattcatcc cggctttttc tgcttcgcca gcgatagtca gcgggttagt gcctaactgc
    10381 ggcgcagacg gcagccagcc catgcgttct gcacggacgt taaagtcgat cagatgtccg
    10441 gtatagcggg atttatccgc catcggcgac agcagttctt ccgcagtcac ggtttcataa
    10501 cgccactggc tggagtggtt atagaaataa gaggtgctgt tcatgtgacg cgccggacgc
    10561 tgccagtcga gggcaaacgc cagcggctgc cagccggttt gcggacgcag tttttcctga
    10621 cctacatagt gcgcccagcc gcccccgctc tgaccgacac agccgcagaa aatcagcatg
    10681 ttgatcagac cacgatagtt catatcgagg tgataccagt ggttcagccc cgcaccgacg
    10741 ataatcatcg aacgaccgtg cgttttatca gcgttatcgg caaattcacg ggcgatgcga
    10801 ataatctggc tgcgagaaac gccggtaatc tgctcggccc aggccggggt ataagctttc
    10861 acatcgtcat agctggttgc acagttaacg tcgttcaggc cacgttccag accgtagttt
    10921 gccagcgtca gatcataaac ggtggtcacc agggcggtgc tgccatcagc cagttgcagg
    10981 cgtttcaccg gcagtttgtg cagcagcacg ttttccagtt ccactttgtt gaagtgttcc
    11041 gtgccgtcgc caccaaagta cgggaagcct acctcagcga tctcatcctg gctacccagc
    11101 aggctcagtt gcagctcggt ttcttcgcca gttttgccgt cgcgctgctc aagattccat
    11161 ttgcccttct cgccccagcg gaagccaata gaaccgttcg gcgcaaccat ttcgccattg
    11221 gtattaaagg cgacagtttt ccattccgga ttgttttcct ggcccagcgc atcaaccaga
    11281 tcagcagcgc gcagcatacg acctgcagcg tagtaaccgt cgcgttcttc cagcatcacc
    11341 agcatcggca tgtcggtgta gcgacgcaca tagtcggtga aatactggct tgggttgtcg
    11401 agatggaatt cacgcagcat tacgtggccc atcgccagcg ccattgccgc atcggtgccc
    11461 tgtttcggtg ccagccacag atcgcacagt ttggcgattt cagcgtagtc tggtgtgacg
    11521 gcaacagttt tggtgccttt gtaacgcact tcagtaaaga agtgagcatc tggagtacgc
    11581 gtctgcggca cgtttgaacc ccaggcgatg atgtagctgg agttgtacca gtcagcagat
    11641 tccggtacgt cagtttgctc gccccaggtt tgcggagacg caggaggcag gtcgcagtac
    11701 cagtcgtaga agcttaagca agtaccgcca atcagcgaga gatagcgtgc acccgatgcg
    11761 taagaaacca tcgacattgc cggaattggc gagaaaccag caacacggtc cgggccgtag
    11821 tttttgatgg tgtaaacgtt agatgcggcg atcagttcgt tcacctcctg ccaggaagaa
    11881 cgaacaaaac caccgcgtcc acgcgcctgc ttaaagcttt tcgctttatc ggcgtcttca
    11941 atgatagaag cccatgcctc aaccggatcg ctatgcagcg ccttcgcttc acgccacatt
    12001 ttcatcaggc gtttgcgcat catcgggtat ttcaggcggt tggcactgta aagataccag
    12061 gagtagctgg caccgcgcgg gcagccgcga ggttcatggt ttggcagatc cggacgggta
    12121 cgcggatagt cagtctgctg ggtttcccag gtgaccagac cgtttttgac gtagattttc
    12181 cagctgcagg agccggtgca gtttaccccg tgggtagagc ggacgatttt gtcatgctgc
    12241 caacgctggc gatatccatc ctcccagtca cggttggtat tgagaagctg gccatgccca
    12301 tcggcaaagg tttcaccctt ctgtttgaag tagcgaaacc ggtccaggaa tttactcatc
    12361 ggttttctcc tgtgggagcc tgtcggccct ctgataaatc gacattgcta atgtttaagg
    12421 gcaaggtaac gctctgaaac agcgtgggaa ttgataacga tcaagagtga tggggatgaa
    12481 aaataaagta aattcctaag ataccccttt aggataatta acagttacag cataacatgc
    12541 tgtaaattaa gacgttttat acctgtagtt atttaaact
//
LOCUS       DBFIFI010000125        12520 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.125, whole genome shotgun sequence.
ACCESSION   DBFIFI010000125 DBFIFI010000000
VERSION     DBFIFI010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12520
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.125"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            193..1707
                     /gene="caiT"
                     /locus_tag="N7B39_003274"
     CDS             193..1707
                     /gene="caiT"
                     /locus_tag="N7B39_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HGU2423198.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFLWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            1738..2880
                     /gene="caiA"
                     /locus_tag="N7B39_003275"
     CDS             1738..2880
                     /gene="caiA"
                     /locus_tag="N7B39_003275"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HGU2423199.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            3009..4226
                     /gene="caiB"
                     /locus_tag="N7B39_003276"
     CDS             3009..4226
                     /gene="caiB"
                     /locus_tag="N7B39_003276"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HGU2423200.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKARETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            4300..5853
                     /gene="caiC"
                     /locus_tag="N7B39_003277"
     CDS             4300..5853
                     /gene="caiC"
                     /locus_tag="N7B39_003277"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HGU2423201.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDTFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYDAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFIDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            5962..6747
                     /gene="caiD"
                     /locus_tag="N7B39_003278"
     CDS             5962..6747
                     /gene="caiD"
                     /locus_tag="N7B39_003278"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HGU2423202.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEESYSYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            6753..7343
                     /gene="caiE"
                     /locus_tag="N7B39_003279"
     CDS             6753..7343
                     /gene="caiE"
                     /locus_tag="N7B39_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HGU2423203.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYTDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(7462..7857)
                     /gene="caiF"
                     /locus_tag="N7B39_003280"
     CDS             complement(7462..7857)
                     /gene="caiF"
                     /locus_tag="N7B39_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HGU2423204.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(8118..11339)
                     /gene="carB"
                     /locus_tag="N7B39_003281"
     CDS             complement(8118..11339)
                     /gene="carB"
                     /locus_tag="N7B39_003281"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HGU2423205.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(11357..12505)
                     /gene="carA"
                     /locus_tag="N7B39_003282"
     CDS             complement(11357..12505)
                     /gene="carA"
                     /locus_tag="N7B39_003282"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HGU2423206.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT         2928 a         3269 c         3316 g         3007 t
ORIGIN      
        1 aacgagaaaa gcgccctcta ttcacccgaa acaaaaatgt gataacaatc acagaatgca
       61 gcttattgaa tacccattat gagttagcca ttaacgcgtc catgaggtta ataataatta
      121 tattaaatgt taacaaaaat aaaacaaacg ggaacgcaaa ataaatattc gttttcacag
      181 tggaattaac tcatgaagaa tgaaaagaga aaaacgggaa tagaaccgaa ggttttcttt
      241 ccgccattaa taatcgtcgg catactttgt tggcttacag tcagagatct ggatgcggcg
      301 aatgtcgtta ttaatgctgt attcagttac gtcaccaatg tatggggatg ggcatttgaa
      361 tggtatatgg tggtgatgct tttcggttgg ttctggctgg tgtttggccc gtatgccaaa
      421 aagcgtttag gtaacgaacc acctgaattt agtaccgcca gttggatctt tatgatgttc
      481 gcctcctgta cgtctgctgc cgtactgttc tggggatcga ttgagatcta ctactacatc
      541 tccaccccgc cgtttggctt agaaccgaac tcgacaggag cgaaagagtt ggggctggct
      601 tacagcttgt tcctctgggg acctctgccg tgggccactt acagcttcct ttcagtcgcc
      661 ttcgcttact tcttctttgt ccgcaaaatg gaagtgattc gccccagctc caccctggtg
      721 ccgctggtag gtgaaaaaca cgccaaaggg ttgttcggca ctatcgtcga caacttctat
      781 ctcgtcgcct tgatcttcgc gatgggtacc agtctgggcc ttgccacgcc gctggtgacc
      841 gagtgtatgc aatggttgtt tggcattccg cataccctgc aactggacgc tatcatcatt
      901 acctgctgga ttatcctcaa cgccatttgc gtcgcctgcg gtctgcaaaa aggggtacgt
      961 atcgccagtg acgtgcgtag ttacctgagc ttcctgatgc tgggttgggt gttcatcgtc
     1021 agcggtgcca gcttcatcat gaactacttc accgattcgg tggggatgtt gctgatgtat
     1081 ctgccgcgca tgttgttcta taccgatccc atcgctaaag gcggcttccc gcagggctgg
     1141 accgtgttct actgggcatg gtgggtgatt tacgccatcc agatgagtat cttcctcgcc
     1201 cgcatctccc gtggtcgtac tgtgcgtgaa ctgtgcttcg gcatggtgat gggactgaca
     1261 gcatccacct ggatcctgtg gactgtactc ggtagtaaca ctctgctgtt gatagataaa
     1321 aacatcatca acattccaaa tctgatcgaa cagtacggtg tggcgcgcgc catcatcgaa
     1381 acctgggccg ctctgccgct cagcaccgcc accatgtggg gcttcttcat cctctgcttt
     1441 attgccaccg ttacgctggt taacgcctgc tcttataccc tggcgatgtc cacttgccgc
     1501 gaagtacgcg atggtgaaga accaccgctg ctggtgcgta tcggttggtc aattctggtt
     1561 ggcattatcg gtattgtttt gctggcgctc ggcggcctga aaccgattca aactgccatt
     1621 atcgccggag gatgcccgct gttcttcgtc aacattatgg tgacgctctc ctttattaaa
     1681 gacgcgaaac agaactggaa agattaatta acccccaaaa tatcaagagg ttgaaagatg
     1741 gattttaatt taaatgatga gcaggaactg tttgtcgccg gtatccgcga actgatggcc
     1801 agcgaaaact gggaggccta ttttgccgag tgcgaccgtg acagcgtcta cccggaacgt
     1861 tttgtcaaag cactggcgga tatgggtatc gacagtctgc tgatccctga agagcacggt
     1921 ggtctggacg cggggtttgt tactctcgcc gccgtgtgga tggagctggg acgtctgggg
     1981 gcaccaacct atgtactgta ccagttgccg ggcgggttca ataccttcct gcgcgaaggc
     2041 acacaagagc agatcgacaa gattatggct ttccgcggca ccggtaagca gatgtggaac
     2101 tcagcgatta ccgaaccggg cgcgggctcc gacgtgggta gcctgaaaac gacttatacc
     2161 cgtagaaatg gtaagattta tcttaatggt agtaagtgtt ttattaccag tagcgcctac
     2221 accccgtaca tcgtggtgat ggcgcgcgac ggggcttctc cggacaaacc tgtctacacc
     2281 gaatggtttg ttgatatgag caaaccgggc atcaaagtga ccaaacttga gaagctcggt
     2341 ctgcgtatgg atagctgctg tgaaatcacc tttgacgatg tggaactgga cgagaaagac
     2401 atgttcggtc gggaaggtaa cggctttaac cgcgtcaaag aagagttcga ccatgaacgt
     2461 ttcctggtag ccctgaccaa ctacggtacg gcgatgtgcg cctttgaaga tgcggcgcgc
     2521 tacgccaacc agcgcgtgca gtttggcgag gctattggtc gtttccagtt gattcaggaa
     2581 aaattcgccc acatggcgat caaattaaac tccatgaaaa acatgctgta tgaagcagcg
     2641 tggaaagcag acaacggcac catcacctct ggcgatgcag cgatgtgcaa atacttctgc
     2701 gccaatgcgg catttgaagt ggtggatagc gcaatgcagg tgctgggcgg tgtcgggatt
     2761 gcgggcaacc accgcatcag ccgcttctgg cgtgacctgc gtgtagaccg cgtttccgga
     2821 ggatctgacg aaatgcagat cctgacgctg ggtcgtgcgg tgctgaagca ataccgctaa
     2881 gtccgtgcgc tgcctgatgc gacgctgacg cgtcttatca ggcctacggg agcacgaatg
     2941 taggccggat aaggcgttta cgccgcatcc ggcagtcatg cgctacataa atttttcagg
     3001 agctaataat ggatcatcta cccatgccga aattcgggcc gttggccgga ttgcgcgttg
     3061 tcttctccgg tatcgaaatt gccgggccgt tcgccgggca aatgttcgca gaatggggtg
     3121 cggaagttat ctggatcgag aacgtcgcct gggccgacac cattcgcgtt caaccgaact
     3181 acccgcaact ctcccgccgc aatttgcacg cgctgtcgtt aaatattttc aaagatgaag
     3241 gccgcgaagc gtttctgaaa ttaatggaaa ccaccgatat cttcatcgaa gccagtaaag
     3301 gtccggcctt tgcccgtcgt ggcattaccg atgaagtact gtggcaacac aacccgaaac
     3361 tggttatcgc tcacctgtcc ggttttggtc agtacggcac cgaggagtac accaatcttc
     3421 cggcctataa caccatcgcc caggccttca gtggttacct gattcagaac ggtgatgttg
     3481 accagccaat gcctgccttc ccgtataccg ccgattactt ttctggcctg accgccacca
     3541 cggcggcgct ggcagcactg cataaagcgc gtgaaaccgg taaaggcgaa agtatcgaca
     3601 tcgccatgta tgaagtgatg ctgcgtatgg gccagtactt catgatggat tacttcaacg
     3661 gcggcgaaat gtgcccgcgc atgagcaaag gtaaagatcc ctactatgcc ggttgcggtc
     3721 tgtataaatg tgccgacggc tacatcgtga tggaactggt gggcattacc caaattgaag
     3781 agtgctttaa agatattggc ctcgcacatc tactcggtac gccggaaatc ccggaaggca
     3841 ctcagcttat ccaccgtatc gaatgccctt acggcccact ggtagaagaa aaactcgatg
     3901 cctggctggc ggcacatacc atcgcagaag ttaaagaacg ctttgccgaa ctgaatatcg
     3961 cctgtgccaa agtactgacc gtaccggaac tggaaagcaa tccacagtat gttgcccgtg
     4021 aatcaatcac tcagtggcaa acgatggatg gtcgcacctg caaagggccg aacatcatgc
     4081 cgaaattcaa aaataacccc ggacaaatct ggcgcggaat gccctcacat ggcatggaca
     4141 cggctgccat tttgaaaaat atcggctaca gcgaaaacga cattcaggag ttggtcagca
     4201 aaggtctggc caaagttgag gactaaacgc gtggcgtcag tcggcttgcc tgactagcgc
     4261 ccgcccaata acaaacgaac aaaaatggat agaggtgcaa tggatatcat tggcggacaa
     4321 catctacgtc aaatgtggga cgatctggcg gacgtttacg gtcataaaac ggcgctgatt
     4381 tgtgaatcca gcggcggagt cgttaaccgg tatagttatc ttgagttaaa tcaggagatt
     4441 aaccgcacgg caaacctgtt ttatacgctg gggattcgca aaggcgacaa ggttgcacta
     4501 catctcgaca actgcccgga atttatcttt tgctggttcg ggctggcaaa aattggcgcg
     4561 attatggtgc cgattaacgc ccgcctgtta cgcgaggaaa gtgcgtggat cctgcaaaat
     4621 agccaggcgt gcctgctggt gaccagtgcg caattctatc ctatgtatca acagattcag
     4681 caagaagatg ccactcaatt gcggcacatt tgcctgacag atgtggcact tcccgctgat
     4741 gatggcgtga gttcgtttac tcaactgaaa aatcaacaac ctgccacctt gtgctatgca
     4801 ccgccgctat cgactgacga tacggcggaa attctcttca cctccggcac cacctcccga
     4861 ccgaaaggtg tggtgattac ccattacaac ctgcgcttcg ctggatatta ctccgcctgg
     4921 cagtgtgcac tgcgtgacga tgacgtctac ctgacggtaa tgcctgcgtt tcatatcgat
     4981 tgccagtgta ctgcggcgat ggcggcgttt tctgccgggg ccacctttgt gctggtcgag
     5041 aaatacagcg cccgcgcctt ctggggacag gtacagaagt accgcgccac cattaccgaa
     5101 tgtattccga tgatgattcg tacgttgatg gtgcagccgc cttcagcgaa cgatcggcaa
     5161 caccgcctgc gggaagtgat gttttatctc aacttgtcgg agcaggaaaa agacacattt
     5221 tgtgaacgct tcggtgttcg cttgctgacg tcttatggga tgacggaaac cattgtgggc
     5281 attatcggcg atcgccctgg cgataaacga cgctggccgt cgattggtcg ggcggggttt
     5341 tgctacgacg cggagatccg cgacgatcac aatcgcccgc tcccggcagg tgagatcggt
     5401 gaaatctgta ttaaaggcgt accagggaaa accatcttca aagagtattt tctcaacccg
     5461 aaagccactg caaaagtgct ggaagctgat ggctggctgc ataccggcga taccggatac
     5521 cgcgacgaag aaggcttttt ttatttcatc gatcgccgct gcaatatgat caaacgcggt
     5581 ggcgagaatg tctcctgcgt ggagctggaa aatatcatcg ccacccatcc aaaaattcag
     5641 gatatcgtgg ttgtgggtat taaagattcg attcgcgatg aagccatcaa agcatttgtg
     5701 gtgctgaatg aaggtgaaac attgagcgaa gaggaatttt tccgcttctg cgaacaaaat
     5761 atggcgaaat ttaaagtgcc ctcttatctg gagatcagaa aagatctgcc acgtaattgc
     5821 tcggggaaaa taattagaaa gaatctgaaa taaatgaaac ggcagggaac taccctgcca
     5881 gctaataatc acgctctaaa acaatatgca ttttttgcag ggatgttgtc gtccctgaaa
     5941 aagcaaaaat ggagaaaagg aatgagtgaa tcattacatc tgacccgcaa tggatcaatt
     6001 ctggaaatta ctcttgatcg cccaaaagcg aatgctattg atgcaaaaac cagctttgaa
     6061 atgggcgaag tatttctaaa tttccgtgac gatccgcaat tacgtgtcgc cattattacc
     6121 ggtgccggag agaaattctt ttccgcgggc tgggatttaa aagcggcagc agaaggcgaa
     6181 gcaccggatg ctgactttgg cccgggtggt tttgcgggat taaccgaaat tttcaatctc
     6241 gacaaaccgg ttatcgcagc tgtgaacggc tacgcctttg gcggcggttt cgaactggcg
     6301 ctggcggcag attttattgt ttgtgccgat aacgccagct tcgccctgcc ggaagccaaa
     6361 ctgggcatcg ttcctgacag cggcggtgtg ctgcgtctgc cgaagatcct gccgcctgcc
     6421 atcgtcaatg aaatggtgat gaccggcaga cgaatgggcg cagaagaggc gctgcgttgg
     6481 gggatagtca accgcgtggt tagccaggcg gaactgatgg ataacgcccg cgaactggct
     6541 cagcagctgg ttaacagcgc cccgctggcg attgcggcgc tgaaagagat ctaccgcacc
     6601 accagcgaaa tgccggtaga agaatcgtat agctatattc gcagcggcgt gttgaaacac
     6661 tatccatcgg ttctgcattc ggaagatgcc attgaagggc cgctggcgtt tgccgagaag
     6721 cgcgatccgg tgtggaaagg acgttaacaa ccgtgagcta ttacgccttt gaggggttaa
     6781 ttccggtggt tcacccgacg gcgtttgtcc atcccagtgc cgtcttgatt ggcgatgtga
     6841 ttgtgggagc cggtgtctac atcggcccac tcgcctcact gcgtggtgac tacgggcggt
     6901 tgatcgtgca agcgggagcc aatattcagg atggctgcat tatgcatggc tacaccgaca
     6961 ctgacaccat cgttggggaa aacggccata tcgggcacgg agcgatcctg catggttgtg
     7021 tgattggtcg cgatgcattg gtcgggatga acagcgtgat tatggatggc gcggtcattg
     7081 gcgaagagag cattgttgcc gccatgagct ttgtcaaagc aggctttcac ggcgagaaac
     7141 gccagttgtt gatgggtacg ccagcccgcg ctgtacgcag tgttagtgac gacgagttac
     7201 actggaaacg gttgaatacc aaagagtatc aggatcttgt cgggcgctgt catgcatcgt
     7261 tacatgaaac acagccactg aggcaaatgg aggaaaatcg cccccgtttg caggggacga
     7321 cggatgtgac gcctaaacgg tgatcttgcc ggatgcggcg taaacgcctt atccaactct
     7381 gctggagagc aaccgtaggc cggataagat gcgccagcat cgcatccggc gaccaggtcg
     7441 cacggtatca tcgaaatgac attaacgacg catactcttt gacaacatca tctgccactt
     7501 ctgctcgcgg ttaagttccg tgagcggaac ggcaggaata cgcggtgtct ttcttacgcc
     7561 gactaatttt tcccgactgt tattgcgcag gcgcgcgtaa atttgctcat cgatatcgac
     7621 cactttaacc agtcgctgac actggcatcc ccgcccttcc agcttattag ggatcatctt
     7681 aacttcgcag cttatttctg tgacttccga cagaatataa gtcagggtat taaccgcctt
     7741 gctgtgttca atatcgaaat gaatagagat ctctcttgct atcacccacc gattttcagc
     7801 catcatccat tcggcgatta acaagtagag tggtttttca acatatcctt cacacatacg
     7861 aactccagtt tcacaaatta aaagtctggc tatgactcgt tttgaagcaa ttaaacaagg
     7921 ccatattaaa attgtggata tatcgatagt atgcaacttc accgtcgata agtgtgagct
     7981 aaatcctgct tatgtcatca aaaatatcga ttccccaaaa gatttactta atgggcaaaa
     8041 aaaacggtcc tcatcagaga accgtattag ttattcgatc aaattagcgg atgaaaaata
     8101 tccgccatga cacgctatta tttgatctgt gcgtgcattt cctgcaccga aattactttt
     8161 tcagtcgcat cggcattcag cgccatcgcg gtagcgaaac caccgttcag ggtggtgtca
     8221 taatgcactt tatattgcag cgcactgcga cggatcacgc gggagtcttc aatcgcacga
     8281 cggcctgagg tggtgttgat gatgtaggta tattcgccat tcttgatacg gtcctgaatg
     8341 tgcggacggc cttcatgcac cttgtttacc agacgcggat tgatacccgc ttcgcccagc
     8401 acaatcgccg tgccgtgggt cgcatccagc tcgaagccct gtttcagcag ttttgccgcc
     8461 aggtccacca cgcgttcttt atcgccttcg cgcacggaaa gcagcgcacg accgtgtttc
     8521 ttcatggtgg agttgctgcc cagctgcgct ttggcaaacg cttcagcgaa ggtgcggccc
     8581 acgcccatga cttccccggt agaacgcatt tctggcccta acagcgggtc aacgcccggg
     8641 aatttattga acggcagcac cacttctttc accgagtagt acggcgggat aacttctttg
     8701 gttacgccct gctcagccag cgatttgcca gccatcacac gcgccgccac ttttgccagc
     8761 ggtacgccgg tggctttgga gacgaacgga acggtacgcg ccgcacgcgg gttaacttca
     8821 atcaggtaga cttcgttgtt tttcaccgca aactgcacgt tcatcaggcc gcgcacctgc
     8881 aattcgaagg ccagtttctg cacctgctgg cgcattacat cctgaatttc ctgacttaag
     8941 gtgtacgctg gcagcgaaca tgcggagtca ccggagtgca cgcccgcctg ctcgatgtgc
     9001 tccatgatgc cgccaatcag caccatttcg ccgtcgcaga tggcatccac gtcaacttct
     9061 actgcgtcat caaggaaatg atccagcaac actggcgcat cgttagacac gctaaccgcc
     9121 gtctggaagt agcgacgcag gtcagcttcg tcatagacga tttccatcgc ccgaccgccg
     9181 agaacgtaag acggacgcac caccagcggg tagccaatct cttttgcctt ctcaactgcc
     9241 atttcaatag cagtaacggt ggcgttcgcc ggttgtttca gtttcagacg gtcaaccgca
     9301 tgctggaagc gttcacggtc ttccgcacgg tcgatagcat ccgggctggt gccgataacc
     9361 ggtacgccag cggcttccag tgcacgcgcc agtttcagcg gggtctgacc gccgtactga
     9421 acgataacgc ctttcggctt ctcaatacgc acgatttcca gcacatcttc cagagttacc
     9481 ggctcgaagt agaggcgatc ggaagtgtcg tagtcggtag agacggtttc cgggttacag
     9541 ttaaccataa tggtttcgta accgtcttca cgcagcgcca gcgaggcgtg tacgcagcag
     9601 tagtcgaatt cgataccctg accgatacgg tttggaccgc cgccaagcac catgattttt
     9661 tcacggtcgg tagacggatt cgcttcgcac tcttcttcat aagtggagta catgtaagcg
     9721 gtgtcggtgg caaactctgc cgcacaggta tccacgcgct tgtagaccgg gtgcaggtca
     9781 tattggtcac gcagcttacg gatttccgct tcgcgtacgc ccgccagttt tgccaagcgc
     9841 gcatcggcaa agcctttgcg tttcagctgg cgcaggaatt cagcgttcag gccagtgatg
     9901 cccacttccg ccactttctc ttccagacgc accagttctt caatctgtac caggaaccag
     9961 cggtcaatgt tagtcaggtt gaagacgccg tccacagaca ggcccgcgcg gaaagcatcg
    10021 gcgatgtacc agatacgctc tgcgcctgcg tctttcagtt cgcgacggat tttggttaac
    10081 gcttccgggt catccaggct cactttcggg tcgaatccag tcgcaccgac ttccaggccg
    10141 cgcagcgctt tttgcaggga ttcctgctgc gtgcgaccaa tcgccatcac ttcgccaacc
    10201 gatttcatct gagtggtcag acggtcgtta gcaccggcga atttttcgaa gttgaagcga
    10261 ggaattttgg taaccacgta atcgatggac ggctcgaagg aggccggagt acgtccgcca
    10321 gtgatgtcgt tcatcagttc atcgagggtg taacccaccg ccagtttcgc cgccacttta
    10381 gcaatcggga aaccggtcgc tttcgacgcc agcgccgaag aacgggacac gcgtgggttc
    10441 atttcgataa caatcaggcg accgtttttc gggttcaccg caaactggac attggaacca
    10501 ccggtttcaa cgccgatttc acgcagcacc gccatcgagg cgttacgcat gatttgatat
    10561 tctttgtcgg tcagcgtttg ggctggcgcg acagtgatgg agtcaccggt gtggatgccc
    10621 atcgcatcaa agttttcgat agagcagacg atgatgcagt tgtcgttttt atcgcgcacc
    10681 acttccatct cgtactcttt ccagccgatc agcgactcat caatcagcaa ctctttggtc
    10741 ggagagagat ccagaccgcg ggcgcaaatt tcttcaaact cttcgcggtt ataagcgata
    10801 ccgccgccgc taccgcccat ggtaaaggat gggcgaataa tgcacgggaa gcccacgtca
    10861 gcggcaaccg ccagcgcttc ttccatcgta tgtgcgatac cggaacgcgc ggtttccaga
    10921 ccaattttct tcatcgctac gtcgaaacga cggcggtctt ctgctttatc aatcgcatcg
    10981 gcagtggcac caatcatggt gacgccgaac tcttccaaca cgccctgacg ttccagctcc
    11041 agcgcgcagt tcagcgccgt ctgaccgccc atggttggca gcaccgcgtc cgggcgctct
    11101 ttttcaataa tcttacgtac cacttcccag tgaatcggct cgatgtaggt cgcatcggcc
    11161 atttccgggt cggtcatgat ggtcgccggg ttggagttca ccagaatgac gcggtaaccc
    11221 tcttcgcgca gggctttaca tgcttgcgcg ccagagtagt caaactcaca cgcctgaccg
    11281 ataacaatcg ggcccgcacc cagaatcagg atacttttta tatctgtacg ttttggcatg
    11341 gctcttttac tcctgattac ttagcggttt tacggtactg ctcaattaac tcgataaagt
    11401 ggtcgaacaa cggcgcggcg tcgtgtggac cagggctggc ttcagggtga ccctggaagc
    11461 tgaacgccgg tttatcggtg cgatgaatgc cctgtaacgt accgtcgaac agggatttat
    11521 gcgtgacacg caggtttgca ggtaatgttg cttcatccac cgcaaaaccg tggttctggg
    11581 cggtgatcat caccacgttt ttctccacat ctttaaccgg atggttgccg ccgtggtgac
    11641 caaatttcat tttgacagtc ttcgcaccgc tcgccagcgc cagcagctga tgaccgaggc
    11701 agatgccgaa gaccggaata tcggtttcga ggaatttctg gatggcggta atggcgtaat
    11761 cgcacggggc cgggtcgcca ggaccgttgg agaggaagat gccgtctgga ttcattttca
    11821 gcacatcttc cgcagaagtt tgcgccggaa cgatggtcag gcgacagcct ctatccacca
    11881 gcatccgcag gatgttgcgt ttggcaccaa aatcataagc cacgacgtgg aacggcagct
    11941 cgtcttcttt tttcgcttct ggcaggccgc cggtcaacgt ccagctccct tgtgtccagc
    12001 tataggcttc tgcggtggtc acttcttttg ccagatccat gccattcaga cctgggaacg
    12061 cgcgggcttt ttctaacgcc agcgccgcat ccgggttatc gcctgcgata atgcagccat
    12121 tctgtgcgcc tttctcgcgc agtaaacgcg tcagcttacg ggtatcgata tcggcaatcg
    12181 ccacgatgtt atggcgcttc aggtaagaag agaggtcttc ggtattacgg aagttgctgg
    12241 caatcagcgg caggtcgcga atcaccagac cttgtgcatg tacctgagaa gattcttcat
    12301 cggcgtcatt agtgccgaca ttgccaatat ggggataagt aagagtaacg atttgacgag
    12361 aataggaagg atcagtgagg atttcttgat aaccggtcat tgaagtattg aaaacgactt
    12421 ccccaaccgc cgaacctgtt gcccctatgg cccgaccgtg aaactgggtt ccgtcttcca
    12481 gaaccaatag cgctgactta atcaaaacac cctccagaga
//
LOCUS       DBFIFI010000126        12391 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.126, whole genome shotgun sequence.
ACCESSION   DBFIFI010000126 DBFIFI010000000
VERSION     DBFIFI010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12391
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.126"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(57..830)
                     /gene="uxuR"
                     /locus_tag="N7B39_003283"
     CDS             complement(57..830)
                     /gene="uxuR"
                     /locus_tag="N7B39_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HGU2423207.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(1045..2505)
                     /locus_tag="N7B39_003284"
     CDS             complement(1045..2505)
                     /locus_tag="N7B39_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HGU2423208.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(2586..3770)
                     /gene="uxuA"
                     /locus_tag="N7B39_003285"
     CDS             complement(2586..3770)
                     /gene="uxuA"
                     /locus_tag="N7B39_003285"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HGU2423209.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SH"
     gene            4110..5453
                     /gene="gntP"
                     /locus_tag="N7B39_003286"
     CDS             4110..5453
                     /gene="gntP"
                     /locus_tag="N7B39_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HGU2423210.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(5627..6529)
                     /gene="fimH"
                     /locus_tag="N7B39_003287"
     CDS             complement(5627..6529)
                     /gene="fimH"
                     /locus_tag="N7B39_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HGU2423211.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(6549..7052)
                     /gene="fimG"
                     /locus_tag="N7B39_003288"
     CDS             complement(6549..7052)
                     /gene="fimG"
                     /locus_tag="N7B39_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HGU2423212.1"
                     /translation="MKWRKRGYLLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(7065..7595)
                     /gene="fimF"
                     /locus_tag="N7B39_003289"
     CDS             complement(7065..7595)
                     /gene="fimF"
                     /locus_tag="N7B39_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HGU2423213.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(7605..10241)
                     /gene="fimD"
                     /locus_tag="N7B39_003290"
     CDS             complement(7605..10241)
                     /gene="fimD"
                     /locus_tag="N7B39_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HGU2423214.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAVQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDRSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRSSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLARNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(10308..11033)
                     /gene="fimC"
                     /locus_tag="N7B39_003291"
     CDS             complement(10308..11033)
                     /gene="fimC"
                     /locus_tag="N7B39_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313302.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HGU2423215.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     IIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGARVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(11070..11609)
                     /locus_tag="N7B39_003292"
     CDS             complement(11070..11609)
                     /locus_tag="N7B39_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2423216.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTPPGGNMQFQGVIIAETCL
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(11674..12222)
                     /gene="fimA"
                     /locus_tag="N7B39_003293"
     CDS             complement(11674..12222)
                     /gene="fimA"
                     /locus_tag="N7B39_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HGU2423217.1"
                     /translation="MKIKTLAIVVLSALSLSSTAALAAATTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLAQDGATSSAVGFNIQLNDCDTNVASKAAVAFLGTAID
                     AGHTNVLALQSSAAGSATNVGVQILDRTGAALTLDGATFSEQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
BASE COUNT         2911 a         3271 c         3049 g         3160 t
ORIGIN      
        1 acttcttaat ttaagaaagc gcaaggaacg tttacccttg cgcttattat aataagtcag
       61 gcgtcgactt tatccagcgg ccatgaatca aacagatagc cgtcaaaata aatatcgtca
      121 acgttcgaga attccagcag acgttgctta acgttttcca gatgctgcca cattgccagc
      181 ttcgccgctc gggcatcttt tttgattaac gcggcgagga tctgtttgtg atcgcccaac
      241 cactctttgc gatacaggct gtcatccaga tggctgtgca actggatcca cattgggttg
      301 ttttcccgcc actgccagga ctgacggaac agctccacca gcatgctgtt atgcgttgct
      361 tcggcaatag cgagatggaa ctgcatgtca ccgctttcgc tgctgcccgg cgcactggaa
      421 gccagttcac gctcttccag ttgcaatgcc tgacgcattt tgacgatatc ttcgcgggta
      481 gcctgcaggg cggcaaactc ggcgatgtta ctttccagta actgacgcgc ctgtaacagc
      541 tcaaaaggac ccgcatcatt gcagacgttg gcatccggac tgtctgtgtt gtggctgcct
      601 gagctatcaa gaacatagat accggcaccc cggcgcactt ccaccagccc tttgatctcc
      661 agcatgatca gcgcttcacg taccaccgtc cgcgtgacat caagcatttc tgcaatttca
      721 cgctccggcg gtagccgttc accaggattg tacggcgtct ttatgatcag atcgcggatc
      781 atcgccccga cttcctggta aggtctttgc gcagaggtgg cagatttcat ggtgttgtgt
      841 ccgttaggtg cggttaatca aatttttgcc ggatgcggcg tgaacgcctt atccggccta
      901 caacgggcaa ttttacggcc cccgttaatc tcaaactata ccattagcac tggaagttgg
      961 cacgtacagg tgggcaaaaa aacgaaaact ggtcaaccaa atatcagttt gattgaccag
     1021 taagtctgta gggtaatcag ttagttacag cgcagccaca cactcgcgcg caccgcgttc
     1081 gcacagctgc tgatatgccg cagtcaccgc gccaacaaag tcggcattct gcggcagatc
     1141 atcggcaaaa ataccgctca ggcccagcag cgctttcacg cggtctgcgc cctgatactg
     1201 cgcgttaatc ttctggaact cggccagcat cgggtcgacc acgtcaatgg cattaccctg
     1261 ctcatccacg ccctgggtgt aacgcatcca gccagccacg cccagcgcca ggtgacgcca
     1321 gctgccgccg ttttgcaggt gcagacgcac cgggtccagc agacgctgcg gtaacttctg
     1381 gctgccgtcc atcgcaatct gccaggtacg gtgacgcaga gacgggttgc tgaaacgctc
     1441 gatcagcagc gtcgcatagg cgttcaggtc cgtaccttcc ggcatcgaca gcgttggcgc
     1501 ttgttcctgc atcatcaggg caaaggccgc tttgcgataa tccgggttag tcatggtgtc
     1561 ggcaatagtt tcatagccgc cgaggtaacc gaggtacgcc aggaaagagt ggctgccgtt
     1621 cagcatacgc agtttcatca tttcgaacgg cacaacgtct gcaacgaact gtgcgcccac
     1681 tttatcccag tccggacgac cattaacgaa gttatcttca ataacccact gacggaacgg
     1741 ttcgcaggca atggcgcacg ggtcgtaaac acccagctgg tcagcaattt cctgtaaggt
     1801 ttctggcgtc gccgccggaa cgatgcggtc aaccatggtg cacgggaagg tgacgttctc
     1861 ttcaatccat gccgctagct gcgggtcacg cgcctgagcc agccccagta ccgcgacctt
     1921 cgccacatga ccgttttcac gcacgttatc gcaggacatc accgtaaatg ctttcagccc
     1981 tttttcacga cgcagacgca aggcttcgac gatgtaacca atcgcggact tcggcgcagt
     2041 cgggttttcc agatcgtgct tgatcagcgg gttattgaga tccagttgac cgcttgccgc
     2101 atcagcgcag tagccttttt ccgtgaccgt cagggagaca atcgccgttt gcggacgcgc
     2161 catcgcgttg agaatacctt cgcagccgtc gatttccggg tgcagcgctt ctttcatcga
     2221 accgataatt ttcagctcgg tgctttccgc gcctttttcc gccacggtgt acagcagttg
     2281 ctgttttttc aggttttcga tcagcacgcg gtcgttgcct ggcatcaggt taacttcgca
     2341 gataccccag tcgctgtcgg tgctttccag cagatgatgg gtgtacagcg cctggtgcgc
     2401 acggtgaaac gccccgcaac cgagatgcac aatgcgtgat tccagacgag aatgatccca
     2461 tgacgggcgg gcaaccggca gattgctgtc aacaatagta gtcatcgtaa actccagaat
     2521 ccgtcttcag agagtgacgg caaggaagaa ggagccggca cggcgcgcag ccatgccggt
     2581 gggtattaat ggctaaagaa agcgcgctgg atcgccagtt cgacaccgcg aacttcggcc
     2641 aggcctttca gacgaccaat tgcggagtaa ccagggttgg ttttcttctt caggtcgtcc
     2701 agcatctgat gaccgtggtc cggacgcatc gggatcaggt cttctttgcc ttccgcttta
     2761 cgacggtgtt cttcttcaac aatcgctttc accacttcgt acatatcaac gtcaccgttc
     2821 aggtgcgccg cttcgtggaa ggttttcggg ttatcttcac gcatggtgga gcgcagatgg
     2881 gtgaagtaaa tacgcggacc gaactgcttg atcatatcaa ccaaatcgtt gtcagcacgc
     2941 acgccgtagg aaccggtgca catggtgaaa ccgttcgcca tgctgtttac ggtatcaacc
     3001 atccactgca tatcttcaat ggtggaaaca atgcgcggca ggccgaggat cgggcgcggc
     3061 ggatcgtccg ggtgaacagc catacggacg ccaacttctt cggcaaccgg aataatcgct
     3121 ttcaggaaga cagcaaagtt ttcgcgcagt ttggctttgt cgatatcttt gtacagctcc
     3181 aggtgcttac ggaactggtc gagggtgtac ccttcttccg cacctggcag accagcgatg
     3241 atgttacggg tcagacgcgc tttgtcttca tcgctcatag tggcgaagcg ttcagctgcc
     3301 tgagcaattt cttcttcggt gtaatccgct tccgcgcctg gacgtttcag gatatgcatt
     3361 tcgaatgcag cgaattcgat ctggtcgaag cgcagagctt tggagccgtc tggcagcacg
     3421 tattcgaggt cagtacgggt ccagtcgagc accggcatga agttgtagca cacggtgcga
     3481 atgccgcact gcgccaggtt gcgcagagtc tgctgatagt tagcgatcca ctgctcatag
     3541 ttgccagtgt gggttttgat atcttcgtga attggtacgc tttctacgac agaccacacc
     3601 aggcctgcgt cttcaacgat cgccttgcgt ttgaggatct cttctacgga ccatacttcg
     3661 ccgttcggga tatggtgcag cgcggtaacc acgccagttg cgcccgcctg acggacatca
     3721 gctaaagaaa ccggatcgtt tgggccgtac cagcgccagg tctgttccat gatttttcct
     3781 ctttcacata gtgggttcca cagcaaagag acgcggaacc ctggagttgt ttaggtatcc
     3841 ggatgaacaa ccagaccgca gccgtggcgt tgcatcggat gagatggcgt ataagttcta
     3901 ccttttggta agacatgtca acccgaattc tgaaattggt taaccacatc acaagaattt
     3961 cactctcatg gttgttgctg caaattgcag caaagggtaa tcgatggtta ttgatcagag
     4021 gatgtgacat tcatcgcaac aatggttgac caatttacat aacatgtcgc ccaaaataac
     4081 aacggttcaa ccaggtaagg taaaacgtta tgcatgtgct taacattctc tgggtggtat
     4141 tcggcattgg tctgatgctg gtactgaatt tgaagttcaa aatcaattca atggtggctt
     4201 tgttggtggc ggcgctgtcc gtcgggatgc tggcgggcat ggatttgatg tcgctgctgc
     4261 acaccatgaa agcgggcttc ggcaacacgc tgggggaact ggctatcatc gtggtgttcg
     4321 gtgcggtcat cggtaaattg atggtcgact ccggcgcggc tcaccagata gcgcatacgc
     4381 tgctggcgcg tctcggtctg cgctatgtac agctgtcggt gattatcatc ggcctgattt
     4441 ttggtctggc gatgttctat gaagtggcct ttatcatgtt agcgccgctg gttattgtca
     4501 ttgccgccga agctaaaatt ccgttcctga aactggcgat cccggcagta gcagctgcca
     4561 ctaccgcaca ttcactgttc ccaccgcagc cgggtccggt ggcgctggtg aatgcttatg
     4621 gcgcagatat ggggatggtt tatatctatg gcgtactggt gacgatccca agtgtaatct
     4681 gcgcaggtct gatcctgccg aagttcctcg gcaatcttga gcgcccaacg ccatcattcc
     4741 tgaaagcaga tcaaccggta gatatgaaca atctgccctc tttcggcgtt tcgattctgg
     4801 tgccgctgat cccggcgatc attatgatct ctaccaccat cgccaatatc tggctggtaa
     4861 aagatacccc tgcctgggaa gtggttaact ttatcggttc ctcgccgatt gcgatgttta
     4921 ttgcgatggt ggttgcattc gtactctttg gcaccgcgcg tggtcatgac atgcagtggg
     4981 tgatgaacgc ttttgaaagc gcggtgaaga gtattgcaat ggtgattctg atcatcggtg
     5041 cgggtggcgt gctgaagcaa actatcatcg acaccggcat tggcgacacc atcggcatgt
     5101 tgatgtccca cggcaatatc tcgccctaca ttatggcatg gctgatcact gtgctaattc
     5161 gtctggcgac gggtcagggt gtcgtttcgg cgatgaccgc cgccgggatt atcagtgctg
     5221 caatccttga tccagcaact ggtcagctgg ttggcgtgaa tccggcgctg ctggtgctgg
     5281 cgacggctgc gggttccaac accctcaccc acattaacga tgcatctttc tggttgttca
     5341 aaggttactt tgacctgtcg gtaaaagaca cgttgaaaac ctggggtctg ctggagctgg
     5401 tcaactccgt ggttgggctg attattgtgc tgattattag catggtagcg taaaagaaca
     5461 ccagagcctg ccaatggcag gctcagttcg gattgtcggc aaagtgcgct ttgtttatgc
     5521 cagatgcgac gctgacgcgt cttatctggc ctacaaaggg ctaacgtgca ggtttttagc
     5581 ttcaggtaat attgcgtacc tgcattagca atgccctgtg atttctttat tgataaacaa
     5641 aagtcacgcc aataatcgat tgcacattcc ctgcagtcac ctgcccgccg gtacgtgcgt
     5701 aatttgccgt taatcccaga cttaccgccg aagttcctac tgctcctaac gataccgtgt
     5761 tattcgctgg aataatcgta ccgttgcgcg tcaactgtac gccgacgccc tgcgctggtg
     5821 aaaacgacgc ggtattggtg aaaatcgagt tgcccgcatc tgcggttgtg ccggagaggt
     5881 aataccccag gttttggctt ttcgcacaat aaacggtaag aggaattggc actgaaccag
     5941 ggtagtccgg cagagtaacg gtgacatcac gagcagaaac atcgcagccg ccagtaggca
     6001 ctaccacatc attattggcg taaatattcc acacaaactg gaaatcatcg ctgttatagt
     6061 tgttggtctg tcgcaaaata agcacggcaa ttaatgagcc agctttaatc gccaccccgc
     6121 ccgcactgct cacaggcgtc aaataaagcg ccaccggcca cggcttatcc gttctcgaat
     6181 tataaacaac ccgcggcgtt tcgctggtgg tcggaaatgg atagctactg ccactatatt
     6241 ttacggtccc ggaaaaatta gataacacgc cgccataagc cgagcctcgt tgcagtgtga
     6301 catagtctgt aatggtttcc ggataatcgt tatggcaaaa gatttgcgtc gaaagatcta
     6361 cgaccaggtt ttgccccaca ttcacggcag gcgcaaggtt tacataaaca ttagcgctgc
     6421 caccgccaat agggatagcg gtaccattgg cggttttaca ggcgaatgac caggcattta
     6481 ccgaccagcc catcagcagt acagcaaaca gggtaataac tcgtttcatt acaattatct
     6541 cttcgggttc agctgtaggt ataggtgatg ctaatcactg cctgaatggt tccctgagtg
     6601 gctccgccat ttaccgtcaa tgctctgacc tgtaacggga agtgcgctga ttgtgaggaa
     6661 tcatccacct gaactgtttt ggttgcgcca gtattcaatg tgttgccact gtcatcctgt
     6721 agctctaact ggatgttttg cgcggtcccc tggtttttat aatatccggt actgtcggct
     6781 gccccgctga agctggcagt gacccttgac gttcccaccg gacaattagt caactcaagc
     6841 gcaacatcat gccaggccga tgccgccccg gcagacatca gactgaaaga ataaagatcg
     6901 ccgagatcaa ccgtggcatt ggtggtggaa actgtgcacg gtttggcgac gaccttaccg
     6961 ttcaccgtga tggtgacatc ggctgcctgt atcgtcgcac ttgcgagcgc caatattgcc
     7021 gccaataaat acccacgttt gcgccatttc atgagcatct ccagttactg atattcaaga
     7081 gtgaaggtag ccgtggcatt gatatgcccc gcagtgacag gcacctgtgt cgccattagc
     7141 cgggcgtaaa aattcaacgt atttggttta cccggcgtca gggtcgtcca cgaaatcgcg
     7201 gacgatggag cattaagggg tatctgattt tgctgctcat tcagaagctg tattcccagt
     7261 cccgaagccg ctgacaccgt attttcaagt gcaagcaggt tggcattgtg gctatctgca
     7321 acgccggtaa acccaacttt tacggcagaa acggcgttac cacagggtga cagcaaaata
     7381 cgaaatggaa cgacaggagt cgtcgcgcca atgttgttaa attgcttcgc cgcgttttcc
     7441 atcagatcaa cagtaaaatt ggttgattca gcggccacac tacagccgtt atctctgaca
     7501 tagccgcgga tagtaatcgt gctatccgca gccaaagcgt gactcactgc cagccacaaa
     7561 aaagcgcaca gaagataaaa aggtttgttt ctcatcacgc ccccttaacg acattcagct
     7621 gatagctggg ttaataactg ctgctgactc tctggtggca gttgataatt ggcgacacaa
     7681 tgagcatttt cctcttctcc ccatttcacc tgaacttttc ccgctagagg cattccgctg
     7741 aggtaaacct gaccattatc cgcaacaatg ccgctactct ggctactctc tgatgtcacc
     7801 atcgccccaa acggcagcgg cttattattg tgggttagcg tcatgagcag ttttatccca
     7861 acgcgcgctt taaactctgc tcgcacgatc gccccacgag tgggaacaac gttagcgacc
     7921 gcgttatcta aatcgacgtt atcagccagg gtattggtat ccagcgccac tctattttcc
     7981 cgatattcag tggcataagg cagcacggca taaccgcgcc agtcggtacg cacccccgtc
     8041 tggttttcga cttttgcatc ttttgcgcca ggcgctttaa caagcaccac cgtatcgttt
     8101 aacggctgcc ccagcgttac gccattggca tgagccagta ccccaccgct gactccgtaa
     8161 tagagctgct taatatcatc gctatggctg taaccgatat tggcattgcc gtaaccaccg
     8221 cgataattca gcgtggcgta gcctgtgctt ccgctattac catcgcctcc cccggcatag
     8281 ccggtttgca cgctatagct gaggttgttg tcttccagca acgtaccgta tacaccagcc
     8341 agattggtca tccgaccgtt gagatcgtgt gacatgctgt agctggcact ggcatgtcgc
     8401 cactgagatt tactgtcaga acgcagccag tggctgaaag gaatattgac gttacgcgct
     8461 aacatctgat cacgtccttt ttgccaggcg tttttcgtca ggctatagct gagcgtccag
     8521 ttgatatctt cgaacgcagt atttaatcca gcctggaatt gctcatcgac attactcgtt
     8581 ccccaataag tttgatggct accactcaaa tacagtgttg atgagcgccc gagttgctga
     8641 gtaacggtga gttgtaattt cccgcgtttg ttataagcga ggttgtaata gtcggtgaat
     8701 ttcggcttaa cctgaataac tccgtcctgt gtttcgatgt tgtagccatt cattcgactg
     8761 tatgttgtat cagcgaaatt aaaatatccg ctggtcgaat aacggtaacc cactaactga
     8821 atattcgtgc ctgactcatt gagcgatttg ttatagagaa aacgcaccga ttgtccgtca
     8881 tgctgactgt catcgggaag tgtggaatta gcctgagtca tatccacaga cagagcgccc
     8941 agtgccccca tatttttccc gataccaaaa ttaaaagcac gataacgatc tgccagttgc
     9001 gttccaccat atattgtcca gccagctgga aggccgtgga gtaatgtact ttggaaaaag
     9061 cggggttttt cctgttgcgc atttccacta cggtattctc ctgccgtaat ggaataacga
     9121 gtatgccctt cacgttgcaa aagcgggact gacgaatagg gtacggtaaa aatctgcgtg
     9181 ctgccgtcag cctctttaat cgttacctgc aagtcaccac tattacctgc ggcatagata
     9241 tcgttgatgg taaaaggccc cggcggcacc gtactattat aaatgtcata cccattttgt
     9301 ttaatagtga cctgtgcagt accacgagca ataccgtgga tcaccggggc aaatcctctt
     9361 tggctatcgg gtaacatatt gtcatctgag gccaattgtg cgccgcgaaa gttaatacca
     9421 tcgaaaatat caccctgagt ataaccatca cccagcgtca gccgggaacg taacggtatt
     9481 atgtctcgct caagccaggt attgatatgc tgccatttat ttttgctacc tgatgatctg
     9541 tcgctactgt tataactcca ggtggtattg tcgcgtaaac gccacgcacc aatatttaac
     9601 ccactctgta ggtttaaata tgcataatgg ctgttacccc caatccgatt ctgtacacta
     9661 tttccgctga aattataatt gagcaatccg gcattaatac cgggatccca taactcagga
     9721 ggaatataac cacgcgcgcg attactcata aatgcctgag ggatcgtcag gttcagtcgc
     9781 tgctgaccaa catctaaatg cgcagtagcg tcctggacca ttgtggttaa tggcacacag
     9841 gcatcatccg ccagcagatt cataccggcg acagaagccg tattcagccc catactggcg
     9901 agttgcgcgc gtgtcaggca gggaacaatc ccttgttcac tgtcgcccgt attaaatgtg
     9961 acatcacgcg ttgccatata accattattc aaatagatat cgacgcgata cgtccctggc
    10021 ggtaattctt gcccattttc aaaacgcgat aaatcggcca cagcctgggg atcatccgct
    10081 aaaaagcgcg gattaaaata gagttcggca gatgacaaag gtgcctgtac ggcaaaagca
    10141 caggcgacaa agagccggac aaaaaaacca gccaaacgat gcttacgaat atgcaagcat
    10201 tgtgtgtttc gctggtaaag tcttaaattc agatatgaca ttattcctcc ggtttaaaat
    10261 cacccggcag tcaattcttt ttattcaggc gaaaaattcc ccctgcgtta ttccattacg
    10321 cccgtcattt tgggggtaag tgcaccataa tcatttattg ttcggtaagt aatattgctt
    10381 cccgcatcag aaggcaattt aaccgtgctt tcgcccattg gaggcaccaa ggcattttca
    10441 agaacccggg ctccggcatt caactctgtt accgtcaggt aatagggtgt cgggttaatc
    10501 agcgtcagag aattcgcgct acgacgaaat cttaattttt ctgcggcctg atcgggtggc
    10561 aacgctaatt tagccggacg atagtacagt ttaatgcggc tgataattgc gagctgtagc
    10621 gtattctcag tcaattttga tttatccatt gacggaatcg ctttaacgtt catccagaat
    10681 aaactttccc ggtcctgtgg caattggtta tttgttgcat caagaatacg taaggtattc
    10741 tctttttttc ccttcatcgc aaacagagga ggcgtcacga taaaacgacc atcctttaca
    10801 ccatcggcat tttccaccca tgattgaatt aaataggtac tattttcatc attatttgtc
    10861 acggcaagtt gcacttgttt ttgccctgcc ggataaatta tgcgagtcgc acctaaggcc
    10921 actcccgctt cagcgcgtcc ggcaaccatc attgccatga acatcaggat acctgccagc
    10981 aagcagaatg ttatttcctg cgatttcctt acattgacgt ttttattact cactgtcctg
    11041 ttcctgttat cacattatct gctgaacaat tactgatagg ttaaagagaa ccaggcctgt
    11101 gcattggcga tgccgccagt aacccgacgc ccggtagcac gatatttggc gatgaaatgt
    11161 agcgaagtag agcctgagta aagccgtttc cagtttgctg gaggacgatt aatcggtacg
    11221 aggtttcctt catcatcaaa caacgctacg ccaatattgg tggctatccc tggcccctct
    11281 cccacggaaa gcacatccgg atttttacca tccgcgacac cgtgaaacgc cacacccaca
    11341 cgttcactca ccaccgtgct acattcccgt aaatgaataa caaaaggcac cggtgcgcta
    11401 tcttccccta ccgcatgaaa ccggttactg ctgatttgcc ccatattgac cgtcatttgt
    11461 ttatcaccgg cttcaatcag gcaagtttcc gcaataatga cgccctgaaa ttgcatattt
    11521 ccgccgggcg gcgtggtatt ccatttattt ccggccagag caaacattgg cagcaacgac
    11581 gctaatagaa ataatctctt ccttttcatc gttattttta tcgcacaagg gtgggcatcc
    11641 ctgcccgtaa tgacgtccct gaacctgggt aggttattga tactgaacct tgaaggtcgc
    11701 atccgcatta gcagcacccg gggttgcctc gccgattgca taataacgcg cctggaacgg
    11761 aatggtgtta gtaccgttat tcagggttgt ttgctcactg aatgtcgcac catccagcgt
    11821 cagcgcagca cccgttctgt ccaggatctg cacaccaacg tttgttgcgc tacccgcagc
    11881 tgaactctgc agagccagaa cgttggtatg acccgcatca atcgccgtac ctaaaaaggc
    11941 aacagcggct ttagatgcaa cattggtatc gcaatcattc agctgaatgt taaaaccgac
    12001 agcagaactg gttgctccgt cctgtgccag cgatgcggta cgaacctgtc ctaactgaac
    12061 ggtttgatca acagagcctg catcaactgc gcaagcggcg ttaacaactt cccctttaaa
    12121 gtgaacggtc ccaccattaa ccgtcgtggc agcggccaga gccgctgtag aactgaggga
    12181 cagagccgac agaacaacga ttgccagagt tttaattttc atgctgcttt cctttcaaaa
    12241 aactatttct aaatcgacat gggcagtcgt tctgtacact ttgttttgtc aacgagtttg
    12301 actgccaaca ctgcacagtt ttcccccaaa agatgaaaca tttggggcca ttttgactca
    12361 tagaggaaag catcgcgcac aaactttttc a
//
LOCUS       DBFIFI010000127        12385 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.127, whole genome shotgun sequence.
ACCESSION   DBFIFI010000127 DBFIFI010000000
VERSION     DBFIFI010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.127"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..1871
                     /gene="gspD"
                     /locus_tag="N7B39_003294"
     CDS             <1..1871
                     /gene="gspD"
                     /locus_tag="N7B39_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HGU2423218.1"
                     /translation="FKDTDIQEFINTVSKNLHKTVIINPDVQGTITVRSYDMLNEEQY
                     YQFFLSVLDVYGFAVVDMHNGILKVVRSKDAKTSAVPVASDVSPGTGDEVVTRVVPVS
                     NVAARDLAPLLRQLNDNAGAGSVVHYEPSNVLLMTGRAAVMKRLMEIVERVDKVGNRS
                     VATVPLTYASATDVARLVTELTKETDKTAIPAWMTAKLVADERTNSVLVSGEPISQQR
                     IISIIKQLDRQEDVQGNTKVIYLKYAKAKDLVEVLTGISSSIENDSKKSPSTEALRKG
                     VTIKSHEQTNALILTGAPDVIRDLENVISQLDIRRPQVLVEAIIAEIQDADGLNLGIQ
                     WVNKHAGVAQFTSTGLPITTMVQTRQNEILDSDQSNALSMFNGIAAGFYQGNWAMLLT
                     ALSTSSKNDILATPSIVTLDNMEATFNVGQEVPVLSGSQTTSGDNIFNTVERKTVGIK
                     LRVKPQINEGDSVLLEIEQEVSGVADTAVATTTDLGATFNTRTVTNAMLVGNGETVVV
                     GGLLDKSIRGSESKVPLLGDIPVLGHLFRAKSEQTAKRNLMLFIRPTIIRERDGFRHA
                     SAEKYQSFNQEQVQSRGKETTALTLNEEQLRLSPDQDDTAFRKVKAAIAAFYAQEM"
     gene            1871..3376
                     /gene="gspE"
                     /locus_tag="N7B39_003295"
     CDS             1871..3376
                     /gene="gspE"
                     /locus_tag="N7B39_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HGU2423219.1"
                     /translation="MSRVVQNVSESRPLLPFSFSRTQRILLLREQEGNRVFCMEDTPA
                     SALLEVRRVAEGPLNVTTVSAEAFEKQLVSSYQRDSDEARQMMAEIGNEMDFYTVAGE
                     LPDREDLLDANDDAPIIRLINAMLTEAIKEKASDIHIETYERHLQVRFRIDGVLREIL
                     RLHRNLASLLISRIKVMARLDIAEKRVPQDGRMVLRIGGRAVDVRVSTLPSNHGERIV
                     LRLLDKNSVSLDLAALGMSQQNQRHIDALIRRPHGIILVTGPTGSGKSTTLYAALSLL
                     NPRDRNIMTVEDPVEYELDGISQTQVNPKVDMTFARSLRAILRQDPDVVLVGEIRDGE
                     TAQIAVQASLTGHLVLSTLHTNSAAGALSRLQDMGIPPFLLSTSLLAVLAQRLVRTLC
                     PRCRQPCQVSTELAMDMDIPPETTIWQPAGCQHCSFTGYHGRTGIHELLLIDDRIRTA
                     IYQGEGELGITRLAGSRYLTLRGDGRQKVLAGETSWEEVVRVTESRLQEEE"
     gene            3378..4601
                     /gene="gspF"
                     /locus_tag="N7B39_003296"
     CDS             3378..4601
                     /gene="gspF"
                     /locus_tag="N7B39_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HGU2423220.1"
                     /translation="MALFHYQASDIHGRKRSGILEADSARHARQLLREQALIPVRLDE
                     KQVHHKHSLRSILRFRPRGGSSAELALLTRQLATLVAASLPLEEALDALLRQSEKPRQ
                     RNLIAAVRTKVLEGHSLAAAMGMFPGTFERLYCAMVAAGETSGRLDVVLSRLADYTEQ
                     RQIMRNRLLQALLYPCVLTLVAVGVIAILLTAVVPKVVEQFIHMKQTLPLSTRVLMGA
                     AEVSQTWGPWLLLAAALGGIAGRMILHQPSQRLAFHHLLLRLPVVGRISRGLNTARYA
                     RTLSILNASAVPLLQAMHISGDVLSNDWARHQLATAAELVREGVSLHQALEQTSLFPP
                     MMRHMIASGENSGELDSMLERAADNQDREFSTQMQLALGLFEPLLVVGMAGVVLFIVL
                     AILQPLLQLNNMMNM"
     gene            4632..5066
                     /gene="gspG"
                     /locus_tag="N7B39_003297"
     CDS             4632..5066
                     /gene="gspG"
                     /locus_tag="N7B39_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HGU2423221.1"
                     /translation="MRKQHQRGFTLLEIMVVIVILGVLASLVVPNLMGNKDKADRQKV
                     MSDLVALESTLDMYRLDNNRYPTTEQGLRALVSKPTVQPEPRNYRQDGYIRRLPQDPW
                     GGDYQLLNPGQYSDIDIFSPGPDGVPNTEDDIGNWTLGNAQP"
     gene            5063..5617
                     /gene="gspH"
                     /locus_tag="N7B39_003298"
     CDS             5063..5617
                     /gene="gspH"
                     /locus_tag="N7B39_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HGU2423222.1"
                     /translation="MSQRGFTILEMMLILLLMGITAGLVLMSFPDSAQNHLQQQRERL
                     QAQLDYALDRSQQDGLLMGIQVRFDGWKFKVLQRGTAESPPTLAEGGDIWQGYVWQTW
                     QPRRAAMGGKLPDDVRLELQYLRGLQWMSEHDDGAEPDILLLPGGEVTPFRLLFRQVG
                     EEAVVGLQVDENGLMTLFEGEVSL"
     gene            5614..5979
                     /gene="gspI"
                     /locus_tag="N7B39_003299"
     CDS             5614..5979
                     /gene="gspI"
                     /locus_tag="N7B39_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HGU2423223.1"
                     /translation="MKGQEGMTLLEVIVALVVFALAGMALMQASTQQAAGIGRMEEKV
                     LAGWLADNQMVQLQLEKTWPENGWGEKTISFAGTEWYLRWGGPDSDVPQPRSLEVEVR
                     RTKEETSALVSLRSSVVRE"
     gene            5976..6575
                     /gene="gspJ"
                     /locus_tag="N7B39_003300"
     CDS             5976..6575
                     /gene="gspJ"
                     /locus_tag="N7B39_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HGU2423224.1"
                     /translation="MSQQRVKGFTLLEMLLALAVFAALSISAFQVLQGGIRAHELSRD
                     KVQRLAELQRGISQMERDLTQMLPRHSRGNEGLLLAAPHLLKSDDWGISFTRNSWLNP
                     AGMLPRPELQWVGYRLRQQKLERLSYFHVDHPSGVSPDVRVMLDGVHAFRLRFFVNGD
                     WQARWDSTGILPQAVEVTLVMDDFAELPRLFLVSKETAE"
     gene            6572..7549
                     /gene="gspK"
                     /locus_tag="N7B39_003301"
     CDS             6572..7549
                     /gene="gspK"
                     /locus_tag="N7B39_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HGU2423225.1"
                     /translation="MKLREQGVALLVVLLILSLMVTIAAVIAERNGRTFLRTVAQLDQ
                     LQAKWDGYTAETIAKQILQRSRQESPRKTHLAQNWAQSERQFETRGGDVRGQIVDAQA
                     CFNLNAINYGVVDLTSIPYAARIFQQLLINLQVELLQARQVTAALRDWIDRDDKPVRG
                     GAEDEVYMGMEPPYLAANQPMQDVSELRLIRGIDARLYRKLLPYVCVLPTSDLSVNVN
                     TLLDSQAPLLAALFLTKPDSLPVTELLQRRPRTGWESVAAFLDAPPLKDIDTSAAMPV
                     LAVSSNYFLVRLHVRSGEHLFSQQTLMQWREERFRIIQRQYGLTMREVP"
     gene            7483..8760
                     /gene="gspL"
                     /locus_tag="N7B39_003302"
     CDS             7483..8760
                     /gene="gspL"
                     /locus_tag="N7B39_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HGU2423226.1"
                     /translation="MEGGAFPDHSATVWINDEGGSLKKNNKHVEAGRLFLRLPEDDSE
                     PLLWRLIAPEETGAGSVAVGETDPQLVGLLSRYPAWVLVPSGRIAFHQVTLPHRTWRQ
                     RLQVLPFLLEEQLATDIEQLHFAILHQSGDRCDVAVVEKAVMHRWLAYCGQLGVREVR
                     LLPDVLMLPLATEGWSAVKLAGQWLFRRDRYAGMTVELSWLTHFLSLTAPPVIESYSV
                     PPVPCPGPKTTEWRNQPGRDLLQLVAEGDGYDGADLRQGEFARQGTWYARFRPWRYVA
                     GALLACVFLAGSNAGLAHYRLWQQAQFWRYESVRVYQQLFPSEKAVKDPRRQMLRHLQ
                     QSTGNNIPELGSVMRQLQQLLSETSTIRLQALAWDSSSQTLKIDLQAASFQALEHFQQ
                     IAGEKYLIQPGEIRQQPQGVESRLILRVNNERA"
     gene            8747..9259
                     /gene="gspM"
                     /locus_tag="N7B39_003303"
     CDS             8747..9259
                     /gene="gspM"
                     /locus_tag="N7B39_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HGU2423227.1"
                     /translation="MNELKKRLQYVSPRERRLLIGCVALLVVVFVYYVLWQPWLIRED
                     KWRTVVTREKNTVEWMKQQVPNIKRREASMPEQGEPLGLSAAVTRTSAAYGVSVTRLQ
                     PQGERLAVTLAPVEFNALMQWLTQLERRHRIRTMVFDVAAQGNPPGQVTVNRLVLSQD
                     DVNKDASLSR"
     gene            9380..10150
                     /locus_tag="N7B39_003304"
     CDS             9380..10150
                     /locus_tag="N7B39_003304"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HGU2423228.1"
                     /translation="MGGVTGSFLNVVIHRLPIMLERRWRREALTLLAHSVPESGPAFN
                     LMIPQSHCPCCFHPLGIRDNIPLLSYLFLKGKSRCCGERIPVYYPLVEITNSVLFILA
                     ASRFPPGLTLAAAWLFISMLLVLAVIDCHTALLPDVLTLPLLWLGLLFSLQRGVVTLE
                     EAVVGAVSGYLCLWGLYWLFRFATGREGLGYGDFKLAAALGAWMGWQALPSILLFASV
                     SGLIVTVLLRIVTATDFTRPLPFGPWLALSGVCHFLLM"
     gene            10242..10643
                     /gene="eptO"
                     /locus_tag="N7B39_003305"
                     /gene_synonym="gspS"
     CDS             10242..10643
                     /gene="eptO"
                     /locus_tag="N7B39_003305"
                     /gene_synonym="gspS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspS family T2SS pilot lipoprotein variant EptO"
                     /protein_id="HGU2423229.1"
                     /translation="MMGNILKKLNCIASLLVLVTISGCHQSPSIHKQATVPPSEQLEQ
                     MASIVSATRYLKMRCNRSDLPDEQSILNVANRIAIGKGWQSLTQEDIRKHSDDIYVRL
                     TRDSTPEYIKCREFNRRLVPFIGELLARGRG"
     gene            10822..11993
                     /locus_tag="N7B39_003306"
     CDS             join(10822..11071,11071..11993)
                     /locus_tag="N7B39_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611522.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGU2423230.1"
                     /translation="MKKRNFSAEFKRESAQLVVDQNYTVADAAKAMDIGLSTMTRWVK
                     QLRDERQGKTPKASPITPEQIEIRELRKKLQRIEMENEIFKKGYNRALDVRLPEQFSI
                     IGKLRAHYPVVTLCHVFGVHRSSYRYWKNRPEKPDGRRAVLRSQVLELHNISHGSAGA
                     RSIATMATLRGFRMGRWLAGRLMKELGLVSCQQPAHRYKRGGREHVTIPNHLGRQFAV
                     TEPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTIKALKMAWEI
                     RSKPAGVMFHSDQGSHYTSRQFRQLLWRYQIKQSLSRRGNCWDNSPMERFFRSLKNEW
                     IPVTGYMNFSDAAHEITDYIVGYYNALRPHEYNGGLPPNESENRYWKNSKAVASFC"
BASE COUNT         2914 a         2823 c         3668 g         2980 t
ORIGIN      
        1 actttaaaga tacggatatt caggagttca taaatactgt cagtaaaaat ttacacaaaa
       61 cggtaataat taatcctgac gtgcagggaa ccatcactgt acgcagctac gatatgctga
      121 acgaggaaca atattatcag ttctttctca gtgtgctgga cgtttatggt tttgctgtgg
      181 tcgatatgca caacggtata ctgaaagtag tgcgctcaaa agatgccaaa acgtcggcgg
      241 tgccggtagc tagtgatgtc agtcccggga ctggtgatga ggttgttacc cgggtggtcc
      301 ccgtaagtaa cgtggcagcc agagatctgg cgcctttgct gcgtcagctc aatgataatg
      361 ctggcgcagg aagcgtggtg cattatgaac cttctaatgt tttgttgatg accggacgtg
      421 ctgcagtgat gaaacggttg atggagattg ttgaacgtgt ggataaggtg ggtaatcgca
      481 gcgttgccac ggtcccgctc acctacgcgt ccgcaacaga cgtagccaga cttgttacgg
      541 aactgactaa agaaacagat aagacagcta tacctgcttg gatgacggcg aaactggttg
      601 cagacgagag gacaaactca gtgctcgtca gcggagagcc aatctcccaa cagcgtatca
      661 tctccataat taagcaactg gatcgtcagg aggatgttca gggtaatact aaggtgattt
      721 acctgaaata tgcgaaggcg aaggatttag tggaagtcct gacaggtatc agcagcagta
      781 ttgaaaacga ctctaaaaag agtccgtcaa cggaagcctt gcgcaaagga gtgacgatta
      841 aatcccacga acaaaccaat gccctgatcc tgacgggggc ccctgacgtc atccgcgacc
      901 ttgaaaatgt gatttcgcag ttggatattc gtcgtcctca ggtcctggtg gaggccatca
      961 ttgctgaaat acaggatgct gacgggctga accttgggat ccagtgggtg aataaacatg
     1021 ccggtgtggc gcagttcacc agtaccggtt tacctattac cacgatggtt cagactcgtc
     1081 agaacgaaat cttagacagc gatcagagca atgccctgag catgtttaac ggaattgcag
     1141 cggggtttta tcagggaaac tgggcgatgc tgttgacggc gctctccaca agtagcaaga
     1201 atgatatctt ggcgaccccc agtattgtca cgctggacaa tatggaggcc actttcaatg
     1261 ttggtcagga ggtcccggta ctttcgggct cacagacaac ctctggggac aatattttta
     1321 acacggtcga gcgcaaaacg gtggggatca aactcagggt aaaaccccag atcaacgagg
     1381 gtgattccgt gttactggag atagaacagg aggtgtccgg tgtggcggac actgcagtag
     1441 ccaccactac tgacttggga gcaaccttca acacccgaac agtgaccaat gccatgctgg
     1501 tcgggaatgg cgaaacggtg gtggtcggag gattactgga taagtcgatc agggggagtg
     1561 agagtaaagt gccactgctg ggggatatcc cggtactggg gcatcttttt cgcgcaaaaa
     1621 gcgaacagac agctaagcgt aatctgatgc tgttcattcg gccaactatt attcgtgagc
     1681 gcgacggatt tcgtcatgct tcggccgaaa aataccagtc gtttaatcag gaacaggtgc
     1741 agtcgcgtgg caaagaaaca acggcgctga cgctgaatga ggaacagctc aggctgtccc
     1801 ccgatcaaga cgatacggct ttccggaagg tgaaagcggc gattgctgcg ttttatgcgc
     1861 aggagatgta atgagcagag tagtacagaa tgtcagtgaa tcacgtcccc ttctgccttt
     1921 ttccttttcc cgtacgcaga gaattttgtt gctgcgtgaa caggagggga atcgagtgtt
     1981 ctgcatggag gacacacctg ccagtgccct gttggaggtg cgcagggtgg ctgaaggtcc
     2041 tctgaatgtc acaacagtgt cggcggaagc gtttgaaaaa cagctggtga gcagctatca
     2101 acgtgattct gatgaagcgc gacaaatgat ggctgagatt ggtaatgaaa tggattttta
     2161 taccgtcgca ggggagttgc ctgaccgtga ggacttactg gatgcgaatg acgatgcgcc
     2221 aattatccgc ctgattaacg caatgctgac agaggcaatt aaagagaaag cctcagatat
     2281 tcatattgaa acttacgaac gccatctgca ggttcgcttt cgcattgatg gcgttctgcg
     2341 ggagatcctc cgtttacata ggaatctggc ttcgttactg atttcacgca ttaaagtcat
     2401 ggcccggttg gatattgccg aaaaacgcgt tccccaagat ggccgcatgg tactgcgtat
     2461 cggtggtcgg gcggtggatg tgcgtgtttc aacgttgcct tcaaatcatg gtgaacgcat
     2521 cgtgctgcgt ttactagaca aaaatagcgt tagtctcgat cttgctgcac tcggcatgtc
     2581 gcagcagaat cagcgacaca ttgatgcact gatccgccgt cctcacggca ttattttggt
     2641 gaccgggcct acagggtcgg gtaaaagcac gacgctttat gccgcgttaa gcctgctgaa
     2701 tccccgagac cgcaacatta tgacggtcga ggatcctgtt gaatatgaac tggacggtat
     2761 cagtcagacc caggtcaacc cgaaggtgga catgaccttt gcccggagtc tgcgcgctat
     2821 tttacgtcag gatccggacg tggtgcttgt gggggagatc cgtgacggtg aaacagccca
     2881 gattgctgtg caggcctcgc tgacagggca tttggtgctg tcaacgttac atacgaatag
     2941 tgcagcaggc gcgctgtcgc gtctgcagga tatgggcatc cctccttttc tgctttccac
     3001 ctcattactg gctgttctgg cccagcgact ggttcgcacg ttgtgtccgc gctgtcgtca
     3061 gccctgtcag gtcagtactg agttagcgat ggacatggac attccccctg aaaccacgat
     3121 ctggcagcct gccgggtgtc agcactgcag tttcacgggc tatcacgggc gcaccgggat
     3181 ccacgagctg ttgctgattg acgatcgcat tcggacggca atctatcagg gggaggggga
     3241 gctgggtatt acccgcttgg cagggagtcg ctatctgacg ctgcgtggcg acgggcgaca
     3301 aaaggtacta gccggtgaga ccagttggga ggaggtggtt cgcgttactg aaagcagatt
     3361 gcaggaagag gaatgatatg gcgttgtttc attatcaggc atcagatatt cacggtagaa
     3421 aacgcagtgg cattctggag gcggattcgg cccgacatgc ccgtcagttg ctgcgtgagc
     3481 aggcgcttat tccagtcagg ttggatgaga aacaggtcca tcacaagcac tcactgcgga
     3541 gtatcctgag gtttcgtccg cgcgggggga gcagcgccga actcgcgtta ctgacacggc
     3601 agttagccac actagtggcc gcatctctgc cgcttgaaga ggcgctggat gcgttgttga
     3661 ggcagagtga aaaaccgcgt cagcggaact taatcgctgc cgtgcgcacc aaggttctcg
     3721 agggacattc cttggccgcg gcgatgggca tgtttccagg tacgtttgag cgtctttatt
     3781 gtgcgatggt ggcggcgggg gaaacatccg gtcgtttgga cgtggtgctc agtcgtctgg
     3841 ctgactatac cgagcagcgc cagatcatgc gaaaccgtct actccaggca ttgctctatc
     3901 cttgtgttct gacgttggtg gcggttgggg ttattgccat tctgcttact gcggttgtgc
     3961 cgaaagtggt tgagcagttt attcatatga aacagaccct tcccttatct acccgcgtac
     4021 tgatgggggc tgctgaggtg agccagactt ggggcccgtg gctgttactg gccgcagcgc
     4081 tgggcgggat agcggggcgc atgatattac atcagccctc ccagcgtctg gcttttcatc
     4141 atctgctgtt gcggctgccg gtcgtggggc gcatatcgcg cggtctgaat accgcacgtt
     4201 atgcccgcac gctgagcatt cttaatgcca gtgcggtacc gctgctccag gcgatgcaca
     4261 tcagtggtga cgtactgagt aatgactggg cccgtcatca gttagctacc gcggccgagt
     4321 tggttaggga aggggtcagc ctgcatcagg cactggagca gacttcgctg tttccgccta
     4381 tgatgcggca catgattgcc agcggtgaaa atagtggcga actcgacagc atgttggaac
     4441 gggccgccga caatcaggat cgtgagttca gcacacagat gcaactggcg ctgggattgt
     4501 ttgaaccgct gttggtggtg ggtatggccg gggtcgtttt gtttattgtt ctggcaattc
     4561 tgcagccgct cctgcagctc aacaacatga tgaatatgtg aatccttctt taacagtcaa
     4621 cggagaaaaa catgcgaaaa cagcatcagc gtggttttac cttgctggaa ataatggtgg
     4681 taattgtgat cttgggagtt ttggccagtc tggtggtccc gaatctgatg ggaaataagg
     4741 ataaggccga tcggcagaaa gtgatgagcg acttggttgc gctggaaagc acgctcgata
     4801 tgtaccggtt agataataac cggtatccga caacggagca gggactgcgg gcactggtca
     4861 gtaaacccac cgtgcaacct gaaccccgta actaccggca ggatggttat atccgccgtt
     4921 tgccccagga tccttggggg ggggattatc agctgttaaa tcctggtcag tacagcgaca
     4981 ttgatatctt ttctcctggc ccggatggtg tgccaaacac tgaagatgat attggtaact
     5041 ggacgctcgg taacgcgcag ccatgagtca gcgcggtttc acaatactgg agatgatgct
     5101 gatccttttg ttgatgggca tcactgcagg gctggtacta atgtcatttc ctgactctgc
     5161 tcagaatcat ctgcaacaac agcgggagcg tctgcaggcg cagctggatt atgccttgga
     5221 tagaagccag caggacggct tgctgatggg gattcaggtt cggtttgacg gatggaaatt
     5281 taaggttctg cagcgcggaa cagcggaaag ccccccgaca ttagctgagg gaggggatat
     5341 ttggcagggg tatgtctggc aaacttggca accgcgacgt gcagccatgg ggggcaaact
     5401 tccggatgat gtgcgtcttg agctgcagta tctcagaggt ttgcagtgga tgtctgagca
     5461 tgatgacggg gctgaacccg atattttgct gcttcccgga ggtgaggtaa cgccgttcag
     5521 acttcttttc aggcaggtcg gagaggaggc cgttgtcggg ctgcaggttg atgagaacgg
     5581 tttgatgact ctttttgaag gtgaggtctc attgtgaagg ggcaagaagg aatgacatta
     5641 ctggaagtca tagtagctct ggttgtgttt gcgctggcag gaatggcgct gatgcaggcc
     5701 tcgacccagc aggctgcggg gatcggacgg atggaagaga aggtgctggc aggctggttg
     5761 gccgataacc agatggtgca attgcaactc gagaaaactt ggccggaaaa tggctggggc
     5821 gagaagacga tcagcttcgc tggcaccgaa tggtatttac gctggggcgg gccggacagc
     5881 gacgtgccgc agccgcgctc cttagaggta gaggtacggc gcactaaaga ggagacgtcc
     5941 gccttggttt ccttgcgtag cagtgtggtt cgtgaatgag tcagcaacgg gtaaaaggtt
     6001 ttacgctact ggaaatgctg ttggcactgg ctgtttttgc agcgttgagc atcagcgcat
     6061 ttcaggtgct gcagggtgga atacgggcgc acgagctgtc ccgggataaa gtacagcgtc
     6121 tggctgagct gcagagaggg atcagccaga tggagcgaga tcttacgcag atgctcccaa
     6181 ggcacagccg ggggaatgaa ggcctgctgc tcgctgcacc tcatctgcta aaaagtgatg
     6241 actgggggat ttcttttacg cgaaacagct ggctgaatcc cgccgggatg ctgccgcgcc
     6301 cggagttaca gtgggtaggt tatcggttgc gacagcaaaa gttagaacgc ctgagctact
     6361 ttcatgtcga tcacccttcc ggtgtttctc cggatgtcag ggtgatgctg gacggcgttc
     6421 acgcatttcg tctgcgtttt tttgttaacg gtgattggca ggcgcgctgg gacagtaccg
     6481 gcatcctgcc gcaggctgtt gaagtgacgc tggttatgga tgattttgct gaactgcccc
     6541 gtctgtttct tgtgagcaag gagaccgctg aatgaaattg cgggaacagg gcgtggcgct
     6601 tctggtggta ttgctgatcc tgtcgttaat ggtaacgata gcggcggtga ttgcggagcg
     6661 caacggtcgg acatttttgc gtacagtagc gcagttggat cagctgcagg caaagtggga
     6721 tggctatacg gcagagacga tagcaaaaca aatcttacag aggagcaggc aggagtcgcc
     6781 ccggaaaacg catctggcac aaaattgggc gcagtcagaa cgacagtttg agaccagagg
     6841 gggagatgtg cgggggcaga tcgtggatgc gcaggcctgt tttaatctta atgctattaa
     6901 ttatggagtg gtggatctga ccagtatacc ttatgccgcc agaatttttc agcagctatt
     6961 aatcaacttg caggtagagc ttttacaggc ccggcaagtg acggcagccc tacgtgactg
     7021 gatcgatcgt gatgataagc cggtaagggg cggtgctgaa gacgaggtgt atatggggat
     7081 ggaacctccc tatctggcag ccaaccagcc gatgcaggac gtcagtgagt tacgccttat
     7141 ccggggaata gatgccaggc tttaccgcaa acttttgccg tatgtttgtg tactgcctac
     7201 gtcagatctg tctgtgaatg tgaatactct gctggattca caggcgccgc tgctggcggc
     7261 gctctttctg acgaaaccgg acagtcttcc ggtgacggaa ttactgcagc ggcgaccccg
     7321 tactggttgg gagagcgtgg cggcgtttct tgacgcgcca ccgcttaagg acattgacac
     7381 cagtgcggcc atgccggtat tggccgtcag cagcaactat tttctggttc ggttacatgt
     7441 gcgctccggg gaacacctgt tcagtcagca gactttaatg cagtggaggg aggagcgttt
     7501 ccggatcatt cagcgacagt atggattaac gatgagggag gttccctgaa aaaaaataac
     7561 aaacatgtcg aggctggtcg gctttttctg cgactgccgg aggatgactc ggagccttta
     7621 ttgtggaggc tgattgcgcc agaggagaca ggagccggca gtgtggcagt cggagaaacg
     7681 gatccgcagt tagtcggttt gctgtcccgt tatcccgcct gggtgctggt tccgtcaggc
     7741 agaattgcct tccatcaggt cacgctgcca caccgcacgt ggcgtcagcg tttgcaggtg
     7801 ctgccctttc ttctggaaga gcagttggcg acagacattg agcagctgca cttcgccatt
     7861 cttcatcagt cgggtgacag gtgtgatgtg gcggtggtcg aaaaagcggt tatgcatcgc
     7921 tggttagcgt actgtggaca acttggggtt cgggaggtga ggttgctgcc tgacgtactg
     7981 atgctgccgt tggcaactga aggatggagt gccgtaaagc ttgccgggca gtggctgttt
     8041 cgccgggatc gttacgccgg gatgacggta gagttatcgt ggctgacaca tttcttgtct
     8101 cttactgcgc cgccggtgat tgagtcctac tccgtgccgc cagtaccgtg cccgggtccg
     8161 aagaccactg agtggcgaaa tcagcccgga cgcgatctgc tgcagttggt ggccgaaggt
     8221 gacgggtatg atggcgcaga tctgcggcag ggagaatttg cccgccaggg aacatggtat
     8281 gcacgatttc gcccatggag gtatgtcgca ggggctctgc tggcctgtgt ttttctggcc
     8341 gggagcaacg cggggctggc acattatcgg ctctggcagc aggctcaatt ctggcggtat
     8401 gagagcgtac gtgtctatca gcagctgttt ccgagtgaaa aggcggtgaa ggatccccgc
     8461 cggcagatgc tgagacatct gcaacagtcg acgggtaaca atataccgga actcgggagc
     8521 gtgatgcgcc agcttcagca actgctgtca gaaacctcaa cgatacgctt acaggcactc
     8581 gcctgggaca gcagcagcca gacactgaaa attgatttgc aggcggcttc ttttcaggcg
     8641 ctggaacact ttcagcagat cgccggagaa aaatacctca tccagcctgg tgagatacga
     8701 cagcagccgc agggggtaga aagtcgtctg atcctgagag tgaacaatga acgagcttaa
     8761 aaaacgtttg cagtatgtta gcccccgtga gcgcaggctg cttatcggat gtgttgcctt
     8821 actggttgtt gtttttgtct attacgtcct ctggcagccc tggctgatac gagaggataa
     8881 gtggcggact gtagttaccc gtgaaaaaaa cacagtggag tggatgaaac aacaggtacc
     8941 caacataaag agacgggagg caagtatgcc tgaacagggg gagccgttag gtttatctgc
     9001 ggccgtgaca cgtacttcag ccgcatacgg tgtatcggtc acccggttgc agcctcaagg
     9061 ggagcgtctg gccgtcacct tagccccggt tgaatttaat gcgctgatgc agtggcttac
     9121 gcaactggag cgtcggcacc gtattcgtac catggtattt gatgtggccg cgcagggcaa
     9181 cccacccgga caggtaacgg ttaatcggct ggttttgtcc caagatgacg taaacaagga
     9241 cgcttcgctt agtcgctgat gtcgtaattt acagattaat gcttgatgta aaaattggac
     9301 ttataaagga ggatgtgtga gtagtttgcc ggaatttgcg gttgattatc ccttgttgtg
     9361 gctcatctgc accggagggc tggggggcgt taccggcagt tttcttaacg tcgtgattca
     9421 tcgtctgccg ataatgttgg agaggcggtg gcggcgtgaa gcgctgacgc tattggcaca
     9481 ttctgtgcct gagtccgggc cggcctttaa cctgatgata ccgcagtctc attgtccctg
     9541 ctgttttcat ccgctcggca tcagggataa cattcccctg ttaagctacc tatttctgaa
     9601 ggggaaatca cgctgttgtg gcgagcgtat tcccgtttac tacccgctag tcgaaataac
     9661 gaactcagtg ttattcattc tggccgcgag caggtttcca ccagggctga cattagcggc
     9721 tgcctggctt tttatttcca tgctgttggt tctggcggtt attgactgcc atacggcgct
     9781 gctgccggat gtgctgacgt tgccattgct ctggcttggc ctgttattca gtttacagag
     9841 aggcgttgtc accttagagg aggcggtagt gggggcggta tctgggtatc tctgtctgtg
     9901 gggactgtac tggctttttc gtttcgctac cgggagggaa gggttaggtt acggtgattt
     9961 taagctagct gcggcattgg gagcatggat gggttggcag gcattaccct caatactttt
    10021 gtttgcctca gtcagtgggt taatcgtaac agtactgttg cgtattgtaa cagctacgga
    10081 ctttacccgc ccgctgcctt ttgggccgtg gctcgctttg agcggagtgt gccatttttt
    10141 gttgatgtag cgttcggtta ccggggctag taataaaaac ggctcagcgg ataaaagaac
    10201 tgttgtaaac cggtatcagg ttaaacgata cctagtaaaa aatgatgggt aatattttga
    10261 aaaaactgaa ttgtatcgcg tcattactag tcttagtgac tatctctggt tgtcatcagt
    10321 caccctccat ccataagcag gccactgttc ctccgagcga acaactggaa caaatggcgt
    10381 cgattgtctc tgcgacacgg tatctgaaaa tgagatgtaa ccgtagtgat ttaccagatg
    10441 aacagtcgat cctaaatgtc gctaaccgga tcgctatcgg gaaagggtgg cagtcattaa
    10501 ctcaggagga tatacgtaaa cacagcgatg atatctatgt gcgtctgact agggacagta
    10561 cgcctgagta tataaaatgc agagaattca atcggcggct ggtcccattt atcggtgaat
    10621 tattggcaag gggtcgtgga taatatgaac cgccccggaa atcctggaga ctaaactccc
    10681 tgagaaagag gtaaacagca tgaataaaaa tgggtcttcc cttgtagtgg tggctgaagg
    10741 catgatgatt gtagtggcac actgaatttg gccacctgaa cagaggtgat atgctcacct
    10801 cagaacaaca caggtgctcc aatgaaaaaa agaaatttca gcgcagagtt taaacgcgaa
    10861 tccgctcaac tggttgttga ccagaactac acggtggcag atgccgccaa agctatggat
    10921 atcggccttt ccacaatgac aagatgggtc aaacaactgc gtgatgagcg tcagggcaaa
    10981 acaccaaaag cctctccgat aacaccagaa caaatcgaaa tacgtgagct gaggaaaaag
    11041 ctacaacgca ttgaaatgga gaatgaaata ttaaaaaagg ctacaaccgc gctcttgatg
    11101 tcagactccc tgaacagttc tcgataatcg ggaaactcag agcgcattat cctgtggtca
    11161 cactctgcca tgtgttcggg gttcatcgca gcagctacag atactggaaa aaccgtcctg
    11221 aaaaaccaga cggcagacgg gctgtattac gcagtcaggt acttgagttg cataacatca
    11281 gccatggttc tgccggggca agaagcatcg ccacaatggc aaccctgaga ggcttcagaa
    11341 tggggcgctg gcttgccggc aggctcatga aagaactggg actggtcagt tgccagcagc
    11401 ctgcgcaccg ttataaacga ggtggtcgtg aacatgtcac tatcccgaat caccttgggc
    11461 ggcagttcgc agtgacagag ccaaatcagg tatggtgcgg cgacgtgacg tacatctgga
    11521 cggggaaacg ttgggcatac cttgccgttg ttctcgacct gtttgcaagg aaaccggtag
    11581 gttgggcaat gtcgttctct ccggacagca gactgaccat caaagcgctg aaaatggcct
    11641 gggaaatccg cagtaaacca gccggggtaa tgttccacag cgatcagggc agccactata
    11701 caagcaggca gttccggcag ttactgtggc gttaccagat caaacagagt ctgagtcgac
    11761 gaggaaattg ctgggataac agcccgatgg agcgcttctt caggagtctg aaaaacgagt
    11821 ggataccggt gacgggttac atgaacttca gcgatgctgc ccatgaaata acggactata
    11881 tcgttgggta ttacaacgcg ctcaggccgc acgaatataa cggtgggttg ccaccaaatg
    11941 aatcggaaaa ccgatactgg aaaaactcta aagcggtggc cagtttttgt tgaccactac
    12001 atcccgtcag ccgatttacg ggccgtaagt aacgaagttg gatgcaaatg tcagatcgtg
    12061 tgcgcctgtt atggctcggc tggtaagaga gccttatagg cgcatttgaa aaaactccag
    12121 ctataccgga agagatttat tattaacagg agaggtttgc ttttctgtgt aggtttagat
    12181 tttgtgtttg cagggtaaac aaaactgatt aactcgtcat ttcggtaact gaaatagttt
    12241 gctgtattct gtttacaatc tctttaataa ggcatttgtc acgtggctat tcatatgaaa
    12301 atcatacgtt atcttacaaa tcaatcatct gagtgttata atataactta gctgtgatat
    12361 gtgtaagaat gtttaggcaa tatat
//
LOCUS       DBFIFI010000128        12183 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.128, whole genome shotgun sequence.
ACCESSION   DBFIFI010000128 DBFIFI010000000
VERSION     DBFIFI010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12183
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.128"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(176..1459)
                     /gene="gltA"
                     /locus_tag="N7B39_003307"
     CDS             complement(176..1459)
                     /gene="gltA"
                     /locus_tag="N7B39_003307"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HGU2423231.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDVKH"
     gene            complement(1665..1772)
                     /locus_tag="N7B39_003308"
     CDS             complement(1665..1772)
                     /locus_tag="N7B39_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2423232.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            2153..2557
                     /gene="sdhC"
                     /locus_tag="N7B39_003309"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             2153..2557
                     /gene="sdhC"
                     /locus_tag="N7B39_003309"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HGU2423233.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            2551..2898
                     /gene="sdhD"
                     /locus_tag="N7B39_003310"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             2551..2898
                     /gene="sdhD"
                     /locus_tag="N7B39_003310"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HGU2423234.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFCASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            2898..4664
                     /gene="sdhA"
                     /locus_tag="N7B39_003311"
     CDS             2898..4664
                     /gene="sdhA"
                     /locus_tag="N7B39_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HGU2423235.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            4680..5396
                     /gene="sdhB"
                     /locus_tag="N7B39_003312"
     CDS             4680..5396
                     /gene="sdhB"
                     /locus_tag="N7B39_003312"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HGU2423236.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            5697..8498
                     /gene="sucA"
                     /locus_tag="N7B39_003313"
     CDS             5697..8498
                     /gene="sucA"
                     /locus_tag="N7B39_003313"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HGU2423237.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            8513..9730
                     /gene="odhB"
                     /locus_tag="N7B39_003314"
     CDS             8513..9730
                     /gene="odhB"
                     /locus_tag="N7B39_003314"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HGU2423238.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            9824..10990
                     /gene="sucC"
                     /locus_tag="N7B39_003315"
     CDS             9824..10990
                     /gene="sucC"
                     /locus_tag="N7B39_003315"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HGU2423239.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            10990..11859
                     /gene="sucD"
                     /locus_tag="N7B39_003316"
     CDS             10990..11859
                     /gene="sucD"
                     /locus_tag="N7B39_003316"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HGU2423240.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
BASE COUNT         2932 a         3098 c         3345 g         2808 t
ORIGIN      
        1 tggtggtgaa tgaaaaatta ggattttatt tagttatcgg atatgtattc ttgtaacgtg
       61 taattatgta tttgtgtacg attacgtttt tttgtcgata gacataacta acgaacaaaa
      121 aaaagcccgc cagatattca ggcgggctaa acgatatatg gttcaaatca gataattaat
      181 gtttaacatc tgatttaaag tctcgttttt catagccggt gtacaactgg cgcggacggg
      241 cgatcttcat gccgtcactg tgcatttcgc tccagtgagc gatccagcca acggtacggg
      301 ccatagcgaa gatgacggtg aacatggaag acggaatacc catcgctttc aggatgatac
      361 cagagtagaa atcgacgttc gggtacagtt tcttctcgat aaagtacggg tcgttcagcg
      421 cgatgttttc cagctccata gccacttcca gcaggtcatc tttggtgccc agctctttca
      481 gaacttcatg gcaggtttca cgcattacgg tggcgcgcgg gtcgtaattt ttgtacacgc
      541 ggtgaccgaa gcccatcagg cggaaagaat catttttatc tttcgcacga cgaacaaatt
      601 ccggaatgtg tttaacggag ctaatttctt ccagcatttt cagcgccgct tcgttagcac
      661 caccgtgtgc aggcccccac agtgaagcaa tacctgctgc gatacaggca aacgggttcg
      721 cacccgaaga gccagcggta cgcacggtgg aggtagaggc gttctgttca tggtcagcgt
      781 gcaggatcag aatacggtcc atagcacgtt ccagaatcgg attaacttca tacggttcgc
      841 acggcgtgga gaacatcata ttcaggaagt taccggcata ggagagatcg ttgcgcggat
      901 aaacaaatgg ctgaccaatg gaatacttgt aacacatcgc tgccatggtc ggcattttcg
      961 acagcaggcg gaacgcggca atttcacgat gacgaggatt gttaacatcc agcgagtcgt
     1021 gatagaacgc cgccagcgcg ccggtaatac cacacatgac tgccattgga tgtgagtcgc
     1081 gacggaaagc gtggaacaga cgggtaatct gctcgtggat catggtatga cgggtcaccg
     1141 tagttttaaa ttcgtcatac tgttcctgag tcggtttttc accattcagc aggatgtaac
     1201 aaacttccag gtagttagaa tcggtcgcca gctgatcgat cgggaaaccg cggtgcagca
     1261 aaataccttc atcaccatca ataaaagtaa ttttagattc gcaggatgcg gttgaagtga
     1321 agccagggtc aaaggtgaac acaccttttg aaccgagagt acggatatca ataacatctt
     1381 gacccagcgt gcctttcagc acatccagtt caacagctgt gtccccgttg agggtgagtt
     1441 ttgcttttgt atcagccatt taaggtctcc ttagcgcctt attgcgtaag actgccggaa
     1501 cttaaatttg ccttcgcaca tcaacctggc tttacccgtt tgttatttgg ctcgccgctc
     1561 tgtgaaagag gggaaaacct gggtacagag ctctgggcgc ttgcaggtaa aggatccatt
     1621 gatgacgaat aaatggcgaa tcaagtactt agcaatccga attattaaac ttgtctacca
     1681 ctaataactg tcccgaatga attggtcaat acttccacac tgttacataa gttaatctta
     1741 ggtgaaatac cgacttcata acttttacgc attatatgct tttcctggta atgtttgtaa
     1801 caactttgtt gaatgattgt caaattagat gattaaaaat taaataaatg ttgttatcgt
     1861 gacctggatc actgttcagg ataaaacccg acaaactata tgtaggttaa ttgtaatgat
     1921 tttgtgaacg gcctatactg ccgccaggtc tccggaacac cctgcaatcc cgagccaccc
     1981 agcgttgtaa cgtgtcgttt tcgcatctgg aagcagtgtt ttgcatgacg cgcagttata
     2041 gaaaggacgc tgtctgaccc gcaagcagac cggaggaagg aaatcccgac gtctccaggt
     2101 aacagaaagt taacctctgt gcccgtagtc cccagggaat aataagaaca gcatgtgggc
     2161 gttattcatg ataagaaatg tgaaaaaaca aagacctgtt aatctggacc tacagaccat
     2221 ccggttcccc atcacggcga tagcgtccat tctccatcgc gtttccggtg tgatcacctt
     2281 tgttgcagtg ggtatcctgc tgtggcttct gggtaccagc ctctcttccc ctgaaggttt
     2341 cgagcaagct tccgcgatta tgggcagctt cttcgtcaaa tttatcatgt ggggcatcct
     2401 taccgctctg gcgtatcacg tcgtcgtagg tattcgccac atgatgatgg attttggcta
     2461 tctggaagaa acattcgaag cgggtaaacg ctccgccaaa atctcctttg ttattactgt
     2521 cgtgctttca cttctcgcag gagtcctcgt atggtaagca acgcctccgc attaggacgc
     2581 aatggcgtac atgatttcat cctcgttcgc gctaccgcta tcgtcctgac gctctacatc
     2641 atttatatgg tcggtttttt cgccaccagt ggcgagctga catatgaagt ctggatcggt
     2701 ttctgcgcct ctgcgttcac caaagtgttc accctgctgg cgctgttttc tatcttgatc
     2761 catgcctgga tcggcatgtg gcaggtgttg accgactacg ttaaaccgct ggctttgcgc
     2821 ctgatgctgc aactggtgat tgtcgttgca ctggtggttt acgtgattta tggattcgtt
     2881 gtggtgtggg gtgtgtgatg aaattgccag tcagagaatt tgatgcagtt gtgattggtg
     2941 ccggcggcgc aggtatgcgc gcggcgctgc aaatttccca gagcggccag acctgtgcgc
     3001 tgctctctaa agtcttcccg acccgttccc ataccgtttc tgcgcaaggc ggcattaccg
     3061 ttgcgctggg taatacccat gaagataact gggaatggca tatgtacgac accgtgaaag
     3121 ggtcggacta tatcggtgac caggacgcga ttgaatatat gtgtaaaacc gggccggaag
     3181 cgattctgga actcgaacac atgggcctgc cgttctcgcg tctcgatgat ggtcgtatct
     3241 atcaacgtcc gtttggcggt cagtcgaaaa acttcggcgg cgagcaggcg gcacgcactg
     3301 cggcagcggc tgaccgtacc ggtcacgcac tgttgcacac gctttatcag cagaacctga
     3361 aaaaccacac caccattttc tccgagtggt atgcgctgga tctggtgaaa aaccaggatg
     3421 gcgcggtggt gggttgtacc gcactgtgca tcgaaaccgg tgaagtggtt tattttaaag
     3481 cccgcgctac cgtgctggcg actggcggag cagggcgtat ttatcagtct accactaacg
     3541 cccacattaa caccggcgac ggcgtcggca tggctatccg cgcaggcgta ccggtgcagg
     3601 atatggaaat gtggcaattc cacccgaccg gcattgccgg tgcgggcgta ctggtcaccg
     3661 aaggttgccg tggtgaaggc ggttatctgc tgaacaaaca tggcgaacgc tttatggagc
     3721 gttatgcgcc gaacgccaaa gacctggcgg gccgtgatgt ggtggcgcgt tcaatcatga
     3781 ttgaaatccg tgaaggtcgc ggctgtgatg gtccgtgggg gccacacgcg aaactgaaac
     3841 tcgatcacct gggtaaagaa gttctcgaat cccgtctgcc gggtatcctg gagctttccc
     3901 gtaccttcgc tcacgttgat ccggtgaaag agccgattcc ggttatccca acctgtcact
     3961 acatgatggg cggtattccg accaaagtta ccggtcaggc gctgactgtg aatgagaaag
     4021 gcgaagatgt ggttgttccg gggctgttcg cggtaggtga aattgcctgc gtatcggtac
     4081 acggtgctaa ccgtctgggc ggcaactcgc tgctggacct ggtggtcttt ggtcgcgcag
     4141 cgggtctgca tctgcaagag tctatcgccg agcagggcgc attgcgcgat gccagcgagt
     4201 ctgatgttga agcatctttg gatcgtctga accgctggaa caataatcgt aacggtgaag
     4261 atccggtggc gattcgtaaa gcgctgcaag aatgtatgca gcataacttc tcggtcttcc
     4321 gtgaaggtga tgcgatggct aaagggcttg agcagttgaa agtgatccgc gagcgtctga
     4381 aaaatgcccg tctggatgac acttccagcg agttcaacac ccagcgcgtt gagtgcctgg
     4441 aactggataa cctgatggaa acggcgtatg caacggctgt ttctgccaac ttccgtaccg
     4501 aaagccgtgg cgcacatagc cgcttcgact tcccggatcg tgatgatgaa aactggctgt
     4561 gccactccct gtatctgcca gagtcggaat ccatgacgcg ccgaagcgtc aacatggaac
     4621 cgaaactgcg cccggcattc ccgccgaaga ttcgtactta ctaatgcgga gacaggaaaa
     4681 tgagactcga gttttcaatt tatcgctata acccggatgt tgatgatgct ccgcgtatgc
     4741 aggattacac cctggaagcg gaagaaggtc gcgacatgat gctgctggat gcgcttatcc
     4801 agctgaaaga gaaagatccc agcctgtcgt tccgccgctc ctgccgtgaa ggtgtgtgcg
     4861 gttccgacgg tctgaacatg aacggcaaga atggtctggc ctgtattacc ccgatttcgg
     4921 cactcaacca gccgggcaag aagattgtga ttcgcccgct gccaggttta ccggtgatcc
     4981 gcgatttggt ggtagacatg ggacaattct atgcgcaata tgagaaaatt aagccttacc
     5041 tgttgaataa tggacaaaat ccgccagctc gcgagcattt acagatgcca gagcagcgcg
     5101 aaaaactcga cgggctgtat gaatgtattc tctgcgcatg ttgttcaacc tcttgtccgt
     5161 ctttctggtg gaatcccgat aagtttatcg gcccggcagg cttgttagcg gcatatcgtt
     5221 tcctgatcga tagccgtgat accgagactg acagccgcct cgacggtttg agcgatgcat
     5281 tcagtgtatt ccgctgtcat agcatcatga actgcgtcag tgtatgtccg aaggggctga
     5341 acccgacgcg cgccatcggc catatcaagt cgatgttgtt gcaacgtaat gcgtaaaccg
     5401 taggcctgat aagacgcgca agcgtcgcat caggcaacca gtgccggatg cggcgtgaac
     5461 gccttatccg gcctacaagt cattacccgt aggcctgata agcgcagcgc atcaggcgta
     5521 acaaagaaat gcaggaaatc tttaaaaact gcccctgaca ctaagacagt ttttaaaggt
     5581 tccttcgcga gccactacgt agacaagagc tcgcaagtga accccggcac gcacatcact
     5641 gtgcgtggta gtatccacgg cgaagtaagc ataaaaaaga tgcttaaggg atcacgatgc
     5701 agaacagcgc tttgaaagcc tggttggact cttcttacct ctctggcgca aaccagagct
     5761 ggatagaaca gctctatgaa gacttcttaa ccgatcctga ctcggttgac gctaactggc
     5821 gttcgacgtt ccagcagtta cctggtacgg gagtcaaacc ggatcaattc cactctcaaa
     5881 cgcgtgaata tttccgccgc ctggcgaaag acgcttcacg ttactcttca acgatctccg
     5941 accctgacac caatgtgaag caggtaaaag tcctgcagct cattaacgca taccgcttcc
     6001 gtggtcacca gcatgcgaat ctcgatccgc tgggactgtg gcagcaagat aaagtggccg
     6061 atctggatcc gtctttccac gatctgaccg aagcagactt ccaggagacc ttcaacgtcg
     6121 gttcatttgc cagcggcaaa gaaaccatga aactcggcga gctgctggaa gccctcaagc
     6181 aaacctactg cggcccgatt ggtgccgagt atatgcacat taccagcacc gaagaaaaac
     6241 gctggatcca acagcgtatc gagtctggtc gcgcgacttt caatagcgaa gagaaaaaac
     6301 gcttcctaag cgaactgacc gccgctgaag gccttgaacg ttacctcggt gcaaaattcc
     6361 ctggcgcaaa acgcttctcg ctggaaggcg gtgacgcgtt aatcccgatg cttaaagaga
     6421 tgattcgcca cgctggcaac agcggcaccc gcgaagtggt tctcgggatg gcgcaccgtg
     6481 gtcgtctgaa cgtgctggtg aacgtgctgg gtaaaaaacc gcaagacttg ttcgacgagt
     6541 ttgccggtaa acataaagaa cacctcggca cgggtgacgt gaaataccac atgggcttct
     6601 cgtctgactt ccagaccgat ggcggcctgg tacatctggc gctggcgttt aacccgtctc
     6661 accttgagat tgtcagcccg gtcgttatcg gttctgttcg tgcccgtctg gacagacttg
     6721 atgagccgag cagcaacaaa gtgctgccaa tcaccattca cggtgacgcc gcagtgaccg
     6781 ggcagggcgt ggttcaggaa actctgaaca tgtcgaaagc gcgtggttat gaagttggcg
     6841 gtacggtacg tatcgttatc aacaaccagg ttggcttcac cacctctaac ccgctggatg
     6901 cccgttcaac gccgtactgt actgatatcg gtaagatggt tcaggcaccg atttttcacg
     6961 ttaacgcgga tgatccggaa gccgttgcct ttgtgacccg tctggcgctc gatttccgta
     7021 acacctttaa acgtgatgtc ttcatcgacc tggtgtgcta ccgccgtcac ggccacaacg
     7081 aagccgacga gccgagcgca acccagccgc tgatgtatca gaaaatcaaa aaacatccga
     7141 cgccgcgcaa aatctatgct gacaagctgg agcaggaaaa agtggcgacg ctggaagatg
     7201 ccaccgagat ggttaacctg taccgcgatg cgctggatgc tggcgattgc gtagtggcag
     7261 agtggcgtcc gatgaacatg cactctttca cctggtcgcc gtacctcaac cacgaatggg
     7321 acgaagagta cccgaataaa gttgagatga agcgcctgca ggagctggcg aaacgcatca
     7381 gcacggtgcc ggaagcggtt gaaatgcagt ctcgcgttgc caaaatttat ggcgatcgcc
     7441 aggcgatggc tgccggtgag aaactgttcg actggggcgg cgcggaaaac ctcgcttacg
     7501 ccacgctggt tgacgaaggc attccggttc gcctgtcggg tgaagactcc ggtcgcggta
     7561 ccttcttcca ccgccacgcg gtgatccaca accagtctaa cggttccact tacacgccgc
     7621 tgcaacacat ccataacggc cagggcgcgt tccgtgtctg ggactccgta ctttctgaag
     7681 aagcagtgct ggcgtttgaa tacggttatg ccaccgcaga accacgcact ctgactatct
     7741 gggaagctca gttcggtgac ttcgccaacg gtgcgcaggt ggttatcgac cagttcatct
     7801 cctctggcga acagaaatgg ggccggatgt gtggtctggt gatgttgctg ccgcacggtt
     7861 acgaagggca ggggccggag cactcctccg cgcgtctgga acgttatctg caactttgtg
     7921 ctgagcaaaa catgcaggtg tgcgtaccgt ctaccccggc acaggtttac cacatgctgc
     7981 gtcgtcaggc gctgcgcggg atgcgtcgtc cgctggtcgt gatgtcgccg aaatccctgc
     8041 tgcgtcatcc gctggcggtt tccagcctcg aagaactggc gaacggcacc ttcctgccag
     8101 ccatcggtga aatcgacgag cttgatccga agggcgtgaa gcgcgtagtg atgtgttctg
     8161 gtaaggttta ttacgacctg ctggaacagc gtcgtaagaa caatcaacac gatgtcgcca
     8221 ttgtgcgtat cgagcaactc tacccgttcc cgcataaagc gatgcaggaa gtgttgcagc
     8281 agtttgctca cgtcaaggat tttgtctggt gccaggaaga gccgctcaat cagggcgcat
     8341 ggtactgcag ccagcatcat ttccgtgaag tgattccgtt tggggcttct ctgcgttatg
     8401 caggccgccc ggcctccgcc tctccggcgg tagggtatat gtccgttcac cagaaacagc
     8461 aacaagatct ggttaatgac gcgctgaacg tcgaataaat aaaggataca caatgagtag
     8521 cgtagatatt ctggtccctg acctgcctga atccgtagcc gatgccaccg tcgcaacctg
     8581 gcataaaaaa cccggtgacg cagtcgtacg tgatgaagtg ctggtagaaa tcgaaactga
     8641 caaagtggta ctggaagtac cggcatcagc agacggcatt ctggatgcgg ttctggaaga
     8701 tgaaggtaca acggtaacgt ctcgtcagat ccttggtcgc ctgcgtgaag gcaacagcgc
     8761 cggtaaagaa accagcgcca aatctgaaga gaaagcgtcc actccggcgc aacgccagca
     8821 ggcgtctctg gaagagcaaa acaacgatgc gttaagcccg gcgatccgtc gcctgctggc
     8881 tgaacataac ctcgacgcca gcgccattaa aggcaccggt gtgggtggtc gtctgacccg
     8941 tgaagatgtg gaaaaacatc tggcgaaagc tccggcgaaa gagtctgcac cggcagcggc
     9001 tgctccggcg gcgcaaccgg ctctggctgc acgtagcgaa aaacgtgtcc cgatgactcg
     9061 cctgcgtaag cgtgtggcag agcgtctgct ggaagcgaaa aactccaccg ccatgctgac
     9121 cacgttcaac gaagtcaaca tgaagccgat tatggatctg cgtaagcagt acggtgaagc
     9181 gtttgaaaaa cgccacggca tccgtctggg ctttatgtcc ttctacgtga aagcggtggt
     9241 tgaagccctg aaacgttacc cggaagtgaa tgcttctatc gacggcgatg acgtggttta
     9301 ccacaactat ttcgacgtca gcatggcggt ttctacgccg cgcggcctgg tgacgccggt
     9361 actgcgtgat gtcgataccc tcggcatggc agacatcgag aagaaaatca aagagctggc
     9421 agttaaaggc cgtgacggca agctgacggt tgaagatctg accggtggta acttcaccat
     9481 caccaacggt ggtgtgttcg gttccctgat gtctacgccg atcatcaacc cgccgcagag
     9541 cgcaattctg ggtatgcacg ctatcaaaga tcgtccgatg gctgtgaatg gtcaggttga
     9601 gatcctgccg atgatgtacc tggcgctgtc ctacgatcac cgtctgatcg atggtcgcga
     9661 atccgtgggc ttcctggtaa cgatcaaaga gttgctggaa gatccgacgc gtctgctgct
     9721 ggacgtgtag tagtttaagt ttcacctgca ctgtagaccg acgtggatag ggtgtcggtc
     9781 tacggtttaa aagataacga ttactgaagg atggacagaa cacatgaact tacatgaata
     9841 tcaggcaaaa caactgtttg cccgctatgg cttaccagca ccggtgggtt atgcctgtac
     9901 tactccgcgc gaagcagaag aagccgcttc aaaaatcggt gccggtccgt gggtagtgaa
     9961 atgtcaggtt cacgctggtg gccgcggtaa agcgggcggt gtgaaagttg taaacagcaa
    10021 agaagacatc cgtgcttttg cagaaaactg gctgggcaag cgtctggtaa cgtatcaaac
    10081 agatgccaat ggccaaccgg ttaaccagat tctggttgaa gcagcgaccg atatcgctaa
    10141 agagctgtat ctcggtgccg ttgtagaccg tagttcacgt cgcgtggtct ttatggcctc
    10201 caccgaaggc ggcgtggaaa tcgaaaaagt ggcggaagaa actccgcacc tgatccacaa
    10261 agttgcgctt gatccgctga ctggcccgat gccgtatcag ggacgcgagc tggcgttcaa
    10321 actgggtctg gaaggtaaac tggttcagca gttcaccaaa atcttcatgg gcctggcgac
    10381 cattttcctg gagcgcgacc tggcgctgat cgaaatcaac ccgctggtca tcaccaaaca
    10441 gggcgatctg atttgcctcg acggcaaact gggcgctgat ggcaacgccc tgttccgcca
    10501 gcctgatctg cgcgaaatgc gtgaccagtc gcaggaagat ccgcgtgaag cacaggctgc
    10561 acagtgggaa ctgaactacg ttgcgctgga cggtaacatc ggttgtatgg ttaacggcgc
    10621 aggcctggcg atgggtacga tggacatcgt taaactgcac ggcggcgaac cggctaactt
    10681 cctggacgtt ggcggcggcg caaccaaaga acgtgtaacc gaagcgttca aaatcatcct
    10741 ctctgacgac aaagtgaaag ccgttctggt taacatcttc ggcggtatcg ttcgttgcga
    10801 cctgatcgct gacggtatca tcggtgcggt agcggaagtg ggtgttaacg taccggtcgt
    10861 ggtacgtctg gaaggtaaca acgccgaact cggcgcgaag aaactggctg acagcggcct
    10921 gaatattatt gcagcaaaag gtctgacgga tgcagctcag caggttgttg ccgcagtgga
    10981 ggggaaataa tgtccatttt aattgataaa aacaccaagg ttatctgcca gggctttacc
    11041 ggtagccagg ggactttcca ctcagaacag gccattgcat acggcactaa aatggttggc
    11101 ggcgtaaccc caggtaaagg cggtactacc cacctcggcc tgccggtgtt caacaccgtg
    11161 cgtgaagctg ttgctgctac tggcgctacc gcttctgtta tctacgtacc agcaccgttc
    11221 tgcaaagact ccattctgga agccatcgac gcaggcatca aactgattat caccatcact
    11281 gaaggcatcc cgacgctgga tatgctgacc gtgaaagtga agctggatga agcaggcgtt
    11341 cgtatgatcg gcccgaactg cccaggcgtt atcactccgg gtgaatgcaa aatcggtatt
    11401 cagcctggtc acattcacaa accgggtaaa gtgggtatcg tttcccgttc cggtacactg
    11461 acctatgaag cggttaaaca gaccacggat tacggtttcg gtcagtcgac ctgtgtcggt
    11521 atcggcggtg acccgatccc tggctctaac tttatcgaca ttctcgaaat gttcgaaaaa
    11581 gatccgcaga ccgaagcgat cgtgatgatc ggtgagatcg gcggtagcgc tgaagaagaa
    11641 gcagctgcgt acatcaaaga gcacgttacc aagccagttg tgggttacat cgctggtgtg
    11701 actgcgccga aaggcaaacg tatgggccac gcgggtgcca tcattgccgg tgggaaaggg
    11761 actgcggatg agaaattcgc tgctctggaa gccgcaggcg tgaaaaccgt tcgcagcctg
    11821 gcggatatcg gtgaagcact gaaaactgtt ctgaaataaa tatctgtaat aagaaatagc
    11881 cctcgccgct tccctctaca ggaatggcga agggctgtcg gtttcgacat ggttggccat
    11941 cgtatgatgg ccttttttat gccttaaacg gttaaccacg gtcgaaatct gactgcaggc
    12001 ttgactgcat gaccacagcc actgtggatt gccgcaaccg accgcaagcc acgaataaga
    12061 acgacggcga atttcgaatc gttacctgat gcactggctt cccagcgttt gaccgtagcg
    12121 ttaagtaaat caacaaaagc gatgttcacc gcttttcgtc aagtcgtgcc atttgtcagc
    12181 cag
//
LOCUS       DBFIFI010000129        11585 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.129, whole genome shotgun sequence.
ACCESSION   DBFIFI010000129 DBFIFI010000000
VERSION     DBFIFI010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11585
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.129"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            275..1663
                     /gene="narU"
                     /locus_tag="N7B39_003317"
     CDS             275..1663
                     /gene="narU"
                     /locus_tag="N7B39_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HGU2423241.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWKNKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAR
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRMKMKGGSDEQA
                     QREAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1745..5485
                     /gene="narZ"
                     /locus_tag="N7B39_003318"
     CDS             1745..5485
                     /gene="narZ"
                     /locus_tag="N7B39_003318"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HGU2423242.1"
                     /translation="MSKLLDRFRYFKQKGDTFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKSLSYKQVRGRGGFIR
                     SNWPELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGKETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLAGGSTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLSPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRICPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5482..7026
                     /gene="narH"
                     /locus_tag="N7B39_003319"
     CDS             5482..7026
                     /gene="narH"
                     /locus_tag="N7B39_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HGU2423243.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIDAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSIAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            7026..7721
                     /gene="narW"
                     /locus_tag="N7B39_003320"
     CDS             7026..7721
                     /gene="narW"
                     /locus_tag="N7B39_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HGU2423244.1"
                     /translation="MQILKVIGLLMEYPDEMLWECKEDALALIRRDAPMLTDFTRNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     TLSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            7718..8398
                     /gene="narI"
                     /locus_tag="N7B39_003321"
     CDS             7718..8398
                     /gene="narI"
                     /locus_tag="N7B39_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HGU2423245.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8477..9370
                     /locus_tag="N7B39_003322"
     CDS             8477..9370
                     /locus_tag="N7B39_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HGU2423246.1"
                     /translation="MEPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTVWQTSLAGK
                     HRVTIEKHHDGYRISLEQGTPGFEPPLEGETRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLRVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGAAVILFHAEWAIKL"
     gene            complement(9466..10310)
                     /gene="nhoA"
                     /locus_tag="N7B39_003323"
                     /pseudo
     CDS             complement(9466..10310)
                     /gene="nhoA"
                     /locus_tag="N7B39_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
     gene            10609..11052
                     /locus_tag="N7B39_003324"
     CDS             10609..11052
                     /locus_tag="N7B39_003324"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HGU2423247.1"
                     /translation="MPVEFEPPRVTIVVDKSTWTRELIERNGKFGIVIPGVAATNWTW
                     AVGSVSGREEDKFNCYGIPVVRGPVFGLPLVEEKCLAWMECRLLPATSAQEEYDTLFG
                     EVVSAAADARVFVEGRWQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11056..11283)
                     /gene="pptA"
                     /locus_tag="N7B39_003325"
     CDS             complement(11056..11283)
                     /gene="pptA"
                     /locus_tag="N7B39_003325"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HGU2423248.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIAPQMEALIKKPGYSM"
     gene            complement(11382..>11585)
                     /locus_tag="N7B39_003326"
     CDS             complement(11382..>11585)
                     /locus_tag="N7B39_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HGU2423249.1"
                     /translation="RLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGLR
                     RKMRKAAMDRNYLASVLAGSGLS"
BASE COUNT         2727 a         3016 c         3038 g         2804 t
ORIGIN      
        1 agttaatata caaccctttg gattcatcat ctgaagggtt gaatcatgac atgcgtacat
       61 agcttaaccc cgtttaatgg ttagcttgtt agttacattt agtaacacat aactggctgc
      121 ttaatcccca ttctgccccc gcggttacgc tttcttatct attctttcaa tcagcacttc
      181 acccatttca tccttaattc taataactgg ctattgcgcc ttacctcctg ttttgtcttt
      241 ttttcattac caatgtgtac atgtgaggaa caatatggca ctgcaaaatg agaaaaatag
      301 tcgttatctt ttacgcgact ggaaaccaga aaatccggcc ttctggaaaa ataaaggaaa
      361 acatattgct cgaagaaacc tctggatatc agtcagttgt ctacttcttg ccttctgtgt
      421 ctggatgcta tttagcgcag ttaccgttaa tctcaataaa atcggtttta atttcactac
      481 cgatcaactc tttttattaa ccgcattacc ctccgtttct ggcgcattat tacgtgtccc
      541 ctactccttt atggtgccta tattcggtgg acgtcgatgg acggttttta gtactgcaat
      601 cctgattatt ccttgcgtct ggctcggaat tgccgtgcag aatccgaata ctccttttgg
      661 gatatttatc gttatcgctt tgctatgcgg ttttgcaggt gcgaactttg cttcgagcat
      721 gggcaatatc agtttcttct ttccaaaagc caggcaaggt agcgctcttg ggattaatgg
      781 cggattagga aacttaggtg taagtgtaat gcagctggtt gcaccgctgg tcatttttgt
      841 acccgtattt gcctttctcg gcgtcaatgg agtaccgcag gccgacggtt cggtgatgtc
      901 gctggcgaat gccgcatgga tttgggtacc gctactagcg attgccacga tcgccgcctg
      961 gtcagggatg aatgatatcg ccagttcacg cgcgtcaatt gccgaccagc tccctgtctt
     1021 acaacgcctg catctctggc tgctgagcct gctttacctt gccaccttcg gttcgtttat
     1081 cggtttttct gcgggtttcg ccatgctggc aaaaacccag ttcccggatg tgaatattct
     1141 gcgcctggcg ttctttggcc catttatcgg tgccatcgcg cggtcggtgg gtggtgctat
     1201 ttccgataaa ttcggcggcg tgcgggtgac gttgatcaac tttattttta tggcgatttt
     1261 cagcgccctg ctgttcctta ccttaccggg cacaggctcc ggtaacttca tcgcctttta
     1321 cgccgtattt atggggctgt ttctgaccgc gggtctggga agtggttcta ctttccagat
     1381 gatcgccgtc atctttcgcc agataaccat ttatcggatg aagatgaaag gcggtagtga
     1441 tgagcaagct cagagagaag ccgtcaccga aacggcggcg gctctgggct ttatctcagc
     1501 catcggcgca gtgggcggct tttttattcc gcaggcattt ggcatgtcac tcaatatgac
     1561 cggctctcca gtgggcgcga tgaaagtgtt tttaatcttc tacatcgttt gtgtgctact
     1621 gacctggctg gtttatggtc ggcggaagtt cagccaaaaa taagagatcg atgcctgatg
     1681 tacaaccgtg tcgcatccga caaggcatca gatcccaatt cattcctgga gcaggagtca
     1741 tgtcatgagt aaacttttgg atcgctttcg ctacttcaaa caaaagggcg atacctttgc
     1801 cgatggtcac ggacaggtga tgcatagcaa ccgcgactgg gaggacagct atcgccagcg
     1861 ttggcagttc gacaaaatcg tgcgttccac ccacggtgtt aactgtacag gctcctgtag
     1921 ctggaaaatc tacgttaaaa atggtctggt gacctgggaa atccaacaga ccgactaccc
     1981 gcgcactcgc cctgacctgc ccaatcatga acctcgcggc tgcccgcgtg gcgcaagtta
     2041 ctcctggtat ctttacagcg ctaaccgcct gaaatacccg ctcattcgta aacgactgat
     2101 tgaactgtgg cgcgaagccc tcaaacaaca cagcgatccg gtactggcgt gggcatcgat
     2161 tatgaacgat ccgcaaaaga gcctgagcta caaacaagtg cgtgggcgcg gcgggtttat
     2221 ccgctccaac tggccggaac taaaccagct gattgccgcc gctaacgtct ggactatcaa
     2281 aacctacggc ccggatcgcg ttgccggttt ctcgccgatc ccggcgatgt cgatggtttc
     2341 ttacgccgcc ggaacacgtt atctgtcact attaggtggc acctgtttaa gcttctacga
     2401 ctggtattgc gacctgccgc ccgcctcgcc gatgacctgg ggcgagcaaa ccgacgtacc
     2461 ggaatctgcc gactggtata actccagtta catcatcgcg tgggggtcta acgtaccgca
     2521 gacacgtacg ccggacgccc atttctttac cgaagtacgc tacaaaggca ctaaaaccat
     2581 cgccattacc cctgactact ctgaagtggc caaattgtgc gaccagtggc tggcaccaaa
     2641 acaaggcact gatagcgccc tggcgatggc aatgggccat gtgattttaa aagagtttca
     2701 tctcgataat cccagcgact actttatcaa ctactgccgc cgctatagcg acatgccgat
     2761 gctggtaatg ctggaacctc gcgacgatgg tagctacgta cccgggcgca tgatccgcgc
     2821 gtctgacctg gtagatggac taggcgaaag caataatccg cagtggaaaa ccgtggcggt
     2881 taataccgca ggtgaattgg tagtgccgaa tggttcgatt ggtttccgct ggggagaaaa
     2941 aggcaaatgg aatctggaat ccattgccgc cggtaaggaa accgaattgt cgttaaccct
     3001 gctcggtcaa catgacgccg ttgcaggcgt ggctttcccc tactttggcg gcatcgaaaa
     3061 tccgcatttt cgcagcgtca aacacaatcc tgtcctggtg cgtcaattac ccgttaaaaa
     3121 cctgacatta gccggtggca gcacctgtcc agtggtcagc gtttatgatt tggtactggc
     3181 gaattacggc ctcgatcgcg ggctggaaga tgaaaacagc gcgaaagatt acgctgaaat
     3241 caaaccgtac accccagcct ggggtgagca aattaccggc gtgccgcgcc agtatattga
     3301 aaccatcgct cgtgaatttg ccgatactgc ccataaaacg catgggcgct cgatgattat
     3361 cctcggcgca ggtgttaacc actggtatca catggacatg aactaccggg gaatgatcaa
     3421 tatgctgatc ttctgcggtt gtgtcggaca aagcggtggc ggctgggcac actatgtcgg
     3481 tcaggaaaaa ctgcgcccgc aaaccggctg gttgccgctg gcctttgcgc tcgactggaa
     3541 ccgaccaccg cgccagatga acagcacctc gtttttctac aatcattcca gccaatggcg
     3601 ctatgaaaaa gtctctgcgc aggagttact ttcaccgctt gccgatgcca gtaagtacag
     3661 cggtcatctg attgatttta acgttcgcgc cgaacgcatg ggctggctac cttctgcgcc
     3721 gcagttgggg cgtaacccgc tcgggattaa agctgaagcc gacaaggccg gattatcccc
     3781 cacagaattt accgcccagg cgctgaaatc gggcgattta cgtatggcct gcgaacaacc
     3841 agatagcggc agcaatcatc cgcgtaattt gtttgtctgg cgttctaacc tgcttggctc
     3901 ctccggcaaa ggccacgagt atatgcagaa gtatctgctg gggaccgaaa gcgggattca
     3961 gggcgaggaa ctcggtgcca gcgacgggat caaaccggaa gaagtcgagt ggcaaactgc
     4021 ggcgattgaa ggcaagctcg acctgctggt gacgctcgac ttccgcatgt ccagtacctg
     4081 cctgttctcc gatatcgttc tgcccaccgc cacctggtat gaaaaagacg atatgaacac
     4141 ctcggatatg catccattta ttcatccgct ttctgcggcg gtcgatccgg cctgggaatc
     4201 acgcagcgac tgggaaatct acaaaggtat tgccaaagca ttttcgcaag tgtgcgtggg
     4261 ccatcttggc aaagaaaccg acgtggtatt acaaccactg ctgcatgact ctccggcaga
     4321 gctctcacag ccgtgtgaag tgctcgactg gcgcaaaggc gaatgcgatc tgagcccggg
     4381 taaaaccgcg ccgaatattg tggcggtgga gcgcgactac cctgctacgt atgaacgctt
     4441 tacctcgctc gggccattga tggacaaact tggcaacggc ggtaaaggga tttcgtggaa
     4501 tacgcaggat gaaatcgatt tcctcggtaa actcaattac accaagcgtg atggcccagc
     4561 gcaggggcgt ccgctgattg acaccgccat tgacgcttca gaagtgattc tggcactggc
     4621 accagaaacc aacggtcatg ttgcagttaa agcgtggcag gcgctgggcg agatcaccgg
     4681 acgcgaacat acccatctgg cgctgcacaa agaggacgag aagattcgct ttcgcgatat
     4741 tcaggcgcag ccgcgtaaaa ttatctccag ccccacatgg tctggtctgg aaagcgatca
     4801 cgtctcctat aatgcgggat acaccaacgt tcatgagtta attccgtggc gcacgctgtc
     4861 gggacgccag cagctctatc aggatcatcc gtggatgcgt gcttttggtg aaagcctggt
     4921 ggcatatcgc ccgcctatcg acacccgtag cgtcagtgag atgcgccaga tcccgccaaa
     4981 cggcttcccg gaaaaagcac ttaacttcct gacgccgcac cagaaatggg gcattcactc
     5041 aacctacagt gaaaacctgc taatgctgac gctctctcgc ggtggaccga ttgtctggat
     5101 cagcgaaacc gatgcccgtg aactaaccat tgtcgataac gactgggtgg aagtgtttaa
     5161 cgccaatggc gcgctgacgg cccgcgcggt ggtcagccaa cgtgtaccgc cgggtatgac
     5221 catgatgtat cacgctcagg aacgcattat gaatattcct ggttcggaag taactggcat
     5281 gcgcggcggt attcataact cggtcacccg catttgcccg aaaccaacgc atatgattgg
     5341 cggttacgcg cagctggcct ggggctttaa ctactacggc accgtcggct cgaaccgcga
     5401 cgaattcatc atgatccgca agatgaagaa cgttaactgg ctggatgatg aaggtcgcga
     5461 tcaggtacag gaggcgaaaa aatgaaaatc cgttcgcaag tcggcatggt gcttaatctc
     5521 gataaatgta tcggctgcca tacctgttcg gtgacctgta aaaacgtctg gaccggacgc
     5581 gaaggcatgg agtacgcatg gtttaacaac gtcgaaacca aaccgggcat tggttatccg
     5641 aaaaactggg aagatcagga agagtggcaa ggcggctggg tccgcgatgt gaatggcaag
     5701 atacgcccgc gtctgggcag caagatgggt gtaataacca aaatcttcgc caacccggtg
     5761 gtgccgcaga ttgatgatta ctacgaacct ttcaccttcg actacgaaca tttgcatagc
     5821 gcaccggaag gcaaacatat ccctactgct cgcccgcgtt cgctgattga cggtaagcgg
     5881 atggacaaag tgatctgggg gccaaactgg gaagaactgc tgggcggcga gtttgaaaaa
     5941 cgtgcccgcg accgcaactt cgaggccatg caaaaggaga tgtacgggca gtttgaaaac
     6001 accttcatga tgtacctgcc gcgcctgtgc gaacactgcc tcaatcccag ctgcgtggcg
     6061 acctgcccaa gcggcgctat ctacaaacgc gaagaagacg gcattgtgct gattgatcag
     6121 gataaatgcc gtggctggcg tttgtgcatt agcggttgtc cgtacaaaaa aatctacttc
     6181 aactggaaaa gcggcaagtc agaaaaatgt atcttctgtt acccacgaat tgagtccggt
     6241 caaccgaccg tgtgctcaga aacctgcgtg ggtcgcatcc gatatctggg cgtgctgctt
     6301 tacgacgctg accgcatcga ggaagcggcg agcaccgagc gcgaggttga cctctatgaa
     6361 cgccagtgcg aagtgttcct cgatccgcac gatccctcag tgatcgagga agccctgaaa
     6421 caaggtattc cacaaaatgt gattgacgct gcccagcgtt cgccagtcta caaaatggcg
     6481 atggactgga aactggcact accgctgcac cctgaatacc gcaccctgcc gatggtctgg
     6541 tacgttcctc cgctgtcacc gattcagtcc tacgcagatg cgggcggttt gccgaaaagc
     6601 gaaggcgtgc tgcccgccat cgaaagcctg cgtattccgg tgcaatatct cgccaatatg
     6661 ttgagtgccg gcgataccgg tccggtactg cgggcgctga aacggatgat ggcgatgcgc
     6721 cactatatgc gttcacaaac cgtggaaggc gttactgata ctcgtgccat cgacgaagta
     6781 ggcctgagca tcgcccaggt cgaagaaatg tatcgctatc tcgccattgc caactatgaa
     6841 gatcgttttg ttatcccgac cagccatcgg gaaatggcgg gcgatgcctt cgcagaacgc
     6901 aatggctgcg gttttacctt tggtgacggt tgtcacggct cggacagcaa attcaacctg
     6961 ttcaacagta gccgtatcga tgccatcaac atcaccgaag tgcgcgacaa agcggagggc
     7021 gaataatgca gatcctcaaa gtgatcggcc tgttgatgga gtatccggac gagatgttgt
     7081 gggaatgcaa ggaggacgcg ctggcgttga tccgccgcga tgcgccgatg cttacggatt
     7141 tcacccgcaa cctgcttaac gcgccgctgc tggataaaca ggccgaatgg tgcgaagtgt
     7201 ttgaccgcgg gcgcaccacg tcgctgctgc tgttcgaaca tgttcatgcc gagtcccgcg
     7261 atcgcggcca ggcaatggtg gacctgctgg cggagtatga aaaggtcggc ctgcagctgg
     7321 attgtcggga actgcccgat tatctaccgc tgtatctgga gtatttaagc gtactgcctg
     7381 acgatcaggc gaaagaagga ttgctcaacg ttgcgccgat cctcgccctg cttggcggtc
     7441 gcttaaaaca acgcgaggca ccgtggtacg cgttgtttga tgctctgctg caactggcgg
     7501 gaagtacact ttcaagtgac agtgtcacaa aacaagtaaa cagcgaagag cgcgatgaca
     7561 cccgccaggc gcttgatgca gtgtgggaag aggaacaggt gaagtttatt gaagataacg
     7621 ccacggcgtg tgacagctcg ccgcttaatc aatatcagcg acgctttagt caggatgtcg
     7681 cgccgcaata tgtcgacatc agtgcgggag gtgggaaatg attcagtatc tgaacgtctt
     7741 tttttacgat atctacccgt acatttgtgc gacggtgttt ttcctcggca gctggctgcg
     7801 ctacgactac gggcagtaca cctggcgcgc ctcctcaagt cagatgctcg ataaacgcgg
     7861 gatggtgata tggtcgaatc tgttccatat cggcattttg gggatcttct tcgggcacct
     7921 gttcggcatg ttaacgccac actggatgta cgcgtggttt ttgccagtgg cagcgaaaca
     7981 actaatggcg atggtgctcg gtggtatttg cggtgtgttg acgttgattg gcggcgcagg
     8041 actgctgtgg cgcaggctga caaatcagcg cgtgcgtgcc acttccacta cgccggatat
     8101 catcatcatg agcattttgc tgatccagtg tctgttgggg ctaagtacca taccgttctc
     8161 agcacagtat cctgatggta gcgaaatgat gaagctggtg ggttgggcgc aaagcattgt
     8221 cactttccgt ggtggatcgt cagagatgct taacggtgta gcgttcgtct tccgcctgca
     8281 tctggtgttg ggaatgacca tcttcttgct cttcccattc acccgactgg tgcatgtgtg
     8341 gagcgccccg tttgagtact ttactcgtcg gtatcagatt gtgcgctcgc ggcgataatt
     8401 tcaccctggc cggatagccc ttcgctctcc ggccacttgc aggctatgat agttctccag
     8461 tcacaggaga acgttaatgg aaccgcaggt ttaccacgtc gatgctttta cctctcaacc
     8521 atttcgcggc aattctgccg gtgttgtttt ccccgccgat aatcttagcg aagcgcaaat
     8581 gcagcttatc gcccgcgaat taggtcattc ggaaaccgct tttctgctgc acagcgacga
     8641 cagcgatgtg cgcatccgct actttacgcc aacagttgaa gtgcctattt gcggtcacgc
     8701 gacagtagct gcgcactatg tacgtgcgaa ggtgttaggt ttaggaaatt gcacggtctg
     8761 gcaaacatcg ctggcaggaa aacatcgcgt gactatcgaa aagcaccacg atggttatcg
     8821 tatttcgctg gaacaaggta cgccaggctt tgagccaccg ctggaaggtg aaacacgtgc
     8881 ggcgattatc aacgcgcttc atctcactga ggacgatatt ctgccaggct tgccaattca
     8941 ggtggcaacc acaggacact caaaagtgat gatcccactg aaaccggaag tggatatcga
     9001 cgccctttcg cctgacctta atgcgctgac cgctatcagc aaacagattg gctgcaatgg
     9061 tttcttcccg ttccagatcc gcccaggcaa aaacgaaacc gatggtcgca tgttctcgcc
     9121 tgcgattggt attgtggaag atccggtgac tggcaatgcc aatggcccga tgggtgcatg
     9181 gttggtacat cacaacgtat tgccccacga tggcaacgtg ttgcgtgtta aaggccatca
     9241 ggggcgcgca ttggggcgtg acggtatgat tgaagtgacg gtgacaattc gtgataacca
     9301 accggaaaaa gtcaccattt ctggcgcggc ggtgattttg tttcatgccg aatgggcaat
     9361 taaactctga aaccgttgcc tgatgcgctt cgcctgtcag gtctacaacc gggctaatgt
     9421 atgccggata aggcgtttac gccgcatccg gcaacctgac ataaattatt ttcccgcctc
     9481 cgggtgcgta tcaaacgccg ccatcaccag cgctaactca tccacggtaa agccatgttt
     9541 cgcatcatcc acgcccagac caaactgttc ttgcatcaca gcatataacg acgccacatc
     9601 cggtagattt cgctgctcca ccgcgtgccc attttcataa tgggaaaatg aaaattggtc
     9661 agtgtcagct tgccgccgtc cggcaaatgg cggcacatca gcaaatgatg gcgaaaatgc
     9721 gactgcggcc agtgcgccga ccagaaatta cccatcacat aatcgctttg ttgctgctcg
     9781 cagagatcaa aacggtacat cgactgccag tgctgatgat gattaaactg caacacccaa
     9841 tcatcaccct cctgcaacaa ccgatactct ccgtgtggcg tggtctgcac aagatcggga
     9901 actaaacgaa tcggcgcggt tagcgtctgc ccaccgaaac cgacatcagc aatccatttt
     9961 tcctcttcca gttccaccaa cagcaaacga tgggtgcgcg gcggtaatgc tggcggattt
    10021 gataacacta cccgccctaa caagctgcga acgttaaacc ccagctcgcg taacacccgc
    10081 tcaaaaacgc cattctgctc aaaacagtaa ccgccacgac gggctatcac cagtttctct
    10141 tccagcgatt gatcatcaag ctgcatttcc ctcggcagca aaacgtcgag gttttcaaac
    10201 ggaatggtgc aattgtgttt caggtgcaat gcacgaagcg tatcaatatt gaccgcagca
    10261 gctcccgacc agttaatacg ggcaaaatag tgattcagaa tgggcgtcat caggcgtttc
    10321 ccttagtggt tttctcaact tataacctgg aatagacgcc gcaccggcta ttttgtgcga
    10381 tcgcggacat gcatcacgtt cgcaaatcgc caatcccgtt aatttcgtta cttattgcgt
    10441 tacggactac gcttaaaaaa accttcttcg cgaggcgata ccatgagccg attcatcccc
    10501 atagaattac accatgccag ccggctgtta aatcatggcc caactgtcat gatcaccagt
    10561 ttcgatgagc aatcccagcg gcgtaacatt atggctgcag cctgatcaat gccggtggag
    10621 tttgaacctc cacgcgtgac gattgtggta gataaatcga catggaccag ggagttgatt
    10681 gaacgtaacg gtaaatttgg catcgttatc ccgggcgttg cagcaactaa ctggacgtgg
    10741 gcagtgggaa gtgtgtcggg tcgtgaagaa gataaattta attgctatgg cattccggtt
    10801 gtgagaggcc cggtatttgg tttgcctctg gtcgaggaaa aatgtctggc gtggatggag
    10861 tgtcgattgc tacctgcgac ttctgcgcaa gaagaatacg acacgctgtt tggcgaagta
    10921 gtatcagcag cggcagacgc acgggtattt gtcgaaggcc gctggcagtt tgatgatgat
    10981 aagctcaata cgttgcatca tttaggtgct gggacgtttg ttaccagcgg caagcgtgtt
    11041 acggcgggtt aagcattaca tgctgtaacc gggtttcttt atcaaagcct ccatttgggg
    11101 cgcgatttcg gcatcccaga tagcttgcca agattctggt tgaatctgtt gtagagcaat
    11161 gcttatcgaa ctgtctttac tgttcagatg gcgaataata acgtcggtaa tatctgcagc
    11221 aagtgctgct ttttgttgtt cgtccagttc acgcggaaaa catttaatgt ctatgtgcgg
    11281 catgattacg ctccttttga aagatgtcag taaaattaac acacattgtg aatgtcgtgt
    11341 gtccggtaaa gcgtttttac gacaataatg ccagggcaag attacgaaag cccgctcccc
    11401 gcaaggactg acgccagata gtttctgtcc atggctgctt ttcgcatctt acgtcttaac
    11461 cctgccttga ataccttatc attcgtcaaa atattaatag caatgtgccg tatccctgaa
    11521 aataattctg ctgcatttcc tcttcttatt ttgcagtcgt cttcattcat taccacgtcc
    11581 agacg
//
LOCUS       DBFIFI010000130        11582 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.130, whole genome shotgun sequence.
ACCESSION   DBFIFI010000130 DBFIFI010000000
VERSION     DBFIFI010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11582
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.130"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            1..312
                     /locus_tag="N7B39_003327"
                     /pseudo
     CDS             1..312
                     /locus_tag="N7B39_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(370..801)
                     /gene="tnpA"
                     /locus_tag="N7B39_003328"
     CDS             complement(370..801)
                     /gene="tnpA"
                     /locus_tag="N7B39_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element IS609 family
                     transposase"
                     /protein_id="HGU2423250.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTRYRRQIFDHDATEK
                     LRTYFSKVCADFEAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGVSGRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCGGAPISAIRQYIEQQQTPG"
     gene            809..2017
                     /locus_tag="N7B39_003329"
     CDS             809..2017
                     /locus_tag="N7B39_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HGU2423251.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDAPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTASQSCGKWY
                     ISIQTENEVSTPVHPSALMVGLDAGVAKLATLSDGTVFGPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQACGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQ
                     EPTEMIQATA"
     gene            2061..2168
                     /locus_tag="N7B39_003330"
                     /pseudo
     CDS             2061..2168
                     /locus_tag="N7B39_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(2152..2790)
                     /gene="ytfB"
                     /locus_tag="N7B39_003331"
     CDS             complement(2152..2790)
                     /gene="ytfB"
                     /locus_tag="N7B39_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HGU2423252.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNYPDQVAPVAPEPIQ
                     EGQPEEQPKTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            3008..3628
                     /gene="fklB"
                     /locus_tag="N7B39_003332"
     CDS             3008..3628
                     /gene="fklB"
                     /locus_tag="N7B39_003332"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HGU2423253.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            3937..5349
                     /gene="cycA"
                     /locus_tag="N7B39_003333"
                     /gene_synonym="dagA"
     CDS             3937..5349
                     /gene="cycA"
                     /locus_tag="N7B39_003333"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HGU2423254.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(5394..6056)
                     /gene="ytfE"
                     /locus_tag="N7B39_003334"
     CDS             complement(5394..6056)
                     /gene="ytfE"
                     /locus_tag="N7B39_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HGU2423255.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHARHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHNTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(6164..7129)
                     /gene="ytfF"
                     /locus_tag="N7B39_003335"
     CDS             complement(6164..7129)
                     /gene="ytfF"
                     /locus_tag="N7B39_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGU2423256.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLACVNIAELNHGLPNFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNTQ
                     TPDFSLPFGPRPLVFISLMIAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            complement(7237..8097)
                     /gene="qorB"
                     /locus_tag="N7B39_003336"
     CDS             complement(7237..8097)
                     /gene="qorB"
                     /locus_tag="N7B39_003336"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313217.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HGU2423257.1"
                     /translation="MIAITGATGQLGQYVIESLMKTVPASQIVAIVRNPAKAQALTAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKTAGVKFIAYT
                     SLLHADKSPLGLADEHIETEKMLADSGIVYTLLRNGWYTENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDDSKTLSKLIGHPTTTLAE
                     SVSHLFNVNN"
     gene            8186..8566
                     /gene="ytfH"
                     /locus_tag="N7B39_003337"
     CDS             8186..8566
                     /gene="ytfH"
                     /locus_tag="N7B39_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HGU2423258.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(8684..10627)
                     /gene="cpdB"
                     /locus_tag="N7B39_003338"
     CDS             complement(8684..10627)
                     /gene="cpdB"
                     /locus_tag="N7B39_003338"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HGU2423259.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINNARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYEPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            10817..11557
                     /gene="cysQ"
                     /locus_tag="N7B39_003339"
     CDS             10817..11557
                     /gene="cysQ"
                     /locus_tag="N7B39_003339"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HGU2423260.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGAKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
BASE COUNT         2728 a         2906 c         3031 g         2917 t
ORIGIN      
        1 aatcagcagg ttaaccagcc acaaaaagcc attgagaaaa ttattgattt tacatgggat
       61 tattatattg ctaacccctg gtttttaaaa attgtgcata gtgaaaatca aagtaaaggt
      121 gtgcattatg caaaatcaca gcgtctctta gaaatcaacc atgcgcactt gcagttaatg
      181 gaatccttat tggatgaagg gaagaaatac aatattttta agccagatat cgacccattg
      241 caggtgaata ttaatattgc tgcgcttggg ggatattatt tgatcaacca gcatacgctt
      301 ggcctggttt atccggtgcg ccgtaaaacc ccgtccttca gggcggggat atacggcgcg
      361 gttttccacc taaccaggtg tttgctgttg ttcaatgtat tgacggatgg cggatattgg
      421 cgcacctccg cagctactgg caaaatagcc aggactccac aaaacgcctt tgtagtaata
      481 cctgactgca atatctggtc gatctcgtcg cagtaaccta cccgatacgc ctttgaggct
      541 gtttaccaga ctggatatcg ccagtttggg aggatagtta attaacaaat ggacgtgatc
      601 tggttcgcca tccatttcaa ccagttcagc ttcaaaatca gcacatacct ttgaaaagta
      661 agtgcgtagt ttttctgtcg cgtcatggtc aaaaatctgg cgtcggtatc tggtgacaaa
      721 gaccaggtga acatgcatca ggaaaacaca atgccttcca cgccggatat cggtttcttt
      781 tttcacagac caaagtatca tttggcctgt gaaacgacta caggcattta aattccagtt
      841 aagacccggt ggtcaacagg agcgtgaaat gaggcgcttc gcaggcgcat gtcgtttcgt
      901 tttcaatcgt gctctggcgc ttcagaatga gaatcatgag gccggtaata aatacatccc
      961 ttacgggaaa atggcttcct ggctggttga gtggaaaaat gccactgaaa cgcaatggct
     1021 taaagatgcc ccgtcacagc cattgcaaca gtcactgaaa gaccttgagc gggcttacaa
     1081 aaacttcttc cggaagcggg ctgcttttcc ccgattcaaa aagcggggac agaatgatgc
     1141 attccgctac ccgcagggcg ttaagctcga tcaggaaaac agccgtattt ttctgccgaa
     1201 acttggctgg atgcgctacc ggaatagccg tcaggtcacg ggtgttgtga aaaatgtcac
     1261 tgccagccag tcctgcggta agtggtacat cagtattcag actgaaaatg aagtatcaac
     1321 tccggttcac ccttcagcat taatggtcgg actggatgct ggcgtggcta aactcgccac
     1381 gctgtcagat ggcacagtct ttggacctgt aaacagtttt cagaaaaacc agaagacgct
     1441 ggcgagactt cagcgacagt taagccgcaa ggtcaaattc agcaacaact ggcagaagca
     1501 gaaacgcaaa atacagcgac tgcattcctg tatcgcaaat atccgcaggg actaccttca
     1561 caaagtcaca acgaccgtca gcaaaaacca cgcaatgata gtcattgagg atttgaaggt
     1621 cagcaacatg tcaaagtcag cagcgggtac ggtcagccag ccggggcgca atgtccgggc
     1681 aaaatcaggt ttaaaccgtt cgatactgga tcagggctgg tatgaaatgc gccgccagct
     1741 tgagtacaag cagctctggc gtggcggtca ggtgcttgct gttccgccag cgtacacaag
     1801 ccagcgttgc gcgtgctgtg gtcatacagc gaaagaaaat cgcctgtcac aaagtaaatt
     1861 cagatgccag gcatgtggat atacagcgaa cgctgatgta aatggcgctc gtaacatttt
     1921 agcggcgggg cacgccgttc ttgcctgtgg ggagatggtg cagtcaggcc gcccgttgaa
     1981 gcaggaaccc accgaaatga ttcaggcgac agcctgaacg tagcagggat ccacgtcctt
     2041 cagggcttgg aggatgtcaa cacatcagta tggtttcacc ccaggcactg gaagccagac
     2101 gtaaggtcat caaagagaca atccttagct ggcttttggt tgacccttca tctaccgcgc
     2161 acgaataaaa ctgccatccg gctggcgggt gaacaggacc tgttgattat tccccgtatc
     2221 aatggttaag cccgtcacca cgccgctggc gttctggcgg attttcacca tctgcccgtt
     2281 ttgcaaatta ctcagcggct tacccgcgcc ttctacctgc gccattgcat agacatcggt
     2341 agcgggcagt ccgtggtcgc ggaacagctg cgccatcgtt ttaccgggtt cgacacggta
     2401 agagcgccat tgattatcaa ttccgctatc tggctggaat ggttgcgttt gggtagtttt
     2461 cggctgttct tctggctggc cttcctgtat aggttctgga gcgaccggag ccacctgatc
     2521 aggataattt tgtggggtga ctaattgcgc ccgcagctgt tcttctgtcg gcggttgtga
     2581 ttgcgactga atatctaact gcgcttcacg cgtcaccacc ggcgcgttgg gcgtatcatc
     2641 agatggaagc agaaatccga ccaccaatac aatggcggca ataatgatcc cccgacgatg
     2701 catcggcggc agcgggtcca tgaagcgaaa attgtccggt gcgtgccaga ctttctccag
     2761 ggttggtttt agttcaaagc gcccgggcat ggtttcctcc tgctccgcgt cttgttcctc
     2821 aatcatagcc tatgaataag ctaacgctat gatgtccgtg gtaaacccgc ctttattata
     2881 ttcatacggg atattgctat tgtttctttt tccctgggat ttgtcatcat tcccgcgaca
     2941 aagttttacc caaagaagtg tggctgatat gctgcccgct actttatacc ctaagaaagg
     3001 aaatacgatg accaccccaa cttttgacac catcgaagcg caagcaagct acggcattgg
     3061 tttgcaggta gggcaacaac tgagtgaatc tggcctggaa gggctgctgc cagaagcact
     3121 ggttgcaggt attgccgatg cgctggaagg caaacatccg gctgttccgg ttgatgtggt
     3181 gcatcgcgcg ctgcgtgaaa tccacgagcg cgccgatgcc gttcgtcgtc agcgtttcca
     3241 ggcgatggct gctgaaggtg tgaaatacct ggaagaaaac gccaaaaaag aaggtgtgaa
     3301 tagcaccgaa tctggcctgc aattccgcgt gatcaaccag ggtgaaggcg caattccggc
     3361 acgtaccgac cgcgttcgtg ttcattacac cggtaaactg atcgacggca ccgtgtttga
     3421 cagctccgtt gctcgtggtg aacccgctga atttccggtt aatggcgtga tccctggctg
     3481 gattgaagca ctgactctga tgccggtagg ttctaaatgg gaactgacta tcccgcagga
     3541 actggcatat ggcgagcgcg gcgcaggcgc atccatccct ccgttcagca ccctggtgtt
     3601 tgaagtcgaa ctgctggaaa tcctctaagc agcgcattct gttcccctcg aaccagaggg
     3661 gagcaggcat tcagcaataa acccttcagt ttgccaaacg gcgctatttt gtgttgcaaa
     3721 gaccccgtaa gcgtgtattt ttgtgagctg tttcgcgtta tcaccgtgat atgacactca
     3781 ctttaaacat aaaattaaca ttagatctaa atcttagtat tcatcccgcg tattgttacc
     3841 taatatcgat gagtcccgat acagattcgt cgtatcatag actgactaaa ggccgtagag
     3901 cctgaacaac acagacaggt acaggaagaa aaaaacatgg tagatcaggt aaaagtcgtt
     3961 gccgatgatc aggctccggc tgaacagtcg ctacggcgca atctcacaaa ccgacatatt
     4021 cagcttattg ccattggtgg tgccattggt acggggttgt ttatggggtc tggcaaaacg
     4081 attagccttg ccgggccgtc gatcattttc gtttatatga tcattggttt tatgctcttt
     4141 ttcgtgatgc gggcaatggg ggaattgctg ctttcgaatc tggaatacaa atcttttagt
     4201 gacttcgctt ccgatttact cgggccgtgg gcaggatatt tcaccggctg gacttactgg
     4261 ttctgctggg ttgtaaccgg tatggcagac gtggtggcga tcacggctta tgctcagttc
     4321 tggttccccg atctctccga ctgggtcgcc tcgctggcgg tgatagtgct gctgctgacg
     4381 cttaacctcg ccactgtgaa aatgttcggt gagatggagt tctggtttgc gatgatcaaa
     4441 atcgtcgcca ttgtgtcgct gattgtcgtc ggcctggtca tggtggcgat gcactttcag
     4501 tcaccgactg gtgtggaagc gtcattcgcg catttgtgga atgacggcgg ctggttcccg
     4561 aaaggtttaa gtggcttctt tgccggattc cagatagcgg ttttcgcttt cgtggggatt
     4621 gagctggtag gtacaacagc tgcggaaacc aaagatccag agaaatcact gccacgcgcg
     4681 attaactcca ttccgatccg tatcattatg ttctacgtct tcgcgctgat tgttattatg
     4741 tccgtgacgc cgtggagttc ggtagtcccg gagaaaagcc cgtttgttga actgtttgtg
     4801 ttggtaggtt tgcctgcggc tgccagcgtg atcaactttg tggtgctgac ctctgcggcg
     4861 tcttccgcta acagtggcgt cttctctacc agccgtatgc tgtttggtct ggcgcaggaa
     4921 ggtgtggcac cgaaagcgtt cgctaaactt tctaagcgcg cagtacccgc gaaagggttg
     4981 actttctcgt gtatctgtct gctcggcggc gtggtgatgt tgtatgtgaa tcctagcgtg
     5041 attggcgcgt tcacgatgat tacaaccgtt tccgcgattc tgtttatgtt cgtctggacg
     5101 attatccttt gctcgtacct tgtgtatcgc aaacagcgtc ctcatctaca tgagaagtcg
     5161 atctacaaga tgccgctcgg caagctgatg tgctgggtat gtatggcgtt ctttgtgttc
     5221 gtggtcgtgt tgctgacact ggaagatgac actcgccagg cgctgctggt taccccgctg
     5281 tggtttatcg cgctggggct gggctggttg tttattggta agaagcgggc tgctgaactg
     5341 cggaaataac cgcattatca tgctggatgg cgcaatgcca tccagctttt agatcactca
     5401 cccgccagcg cccttgggaa cagtacattg ttttccaggc tgatgtgatc catcaggtca
     5461 tcaatcagtt cattaatgcc gttatacatc gctttccagg tggtgcaggc ttccggcggc
     5521 ggtgtgacgt tattggtatt gtgtttgatc acttccagca gttcacccgc ttcatcgtgc
     5581 tcgctttcca ttacgctgat tggccccatt gcctggctgc ccatgccttg tttgatcatc
     5641 gggaagagga tctgctcttc tttcatcatg tggctggaaa gctcttcatg cagcatggtc
     5701 aggtattttg tcagcccttt tggcacgctc ggtttgtcgg cgtgaacgcg ctcgacttta
     5761 gtcgcttgca gaatcagctc cggcagttgc tcgcggtgac gagcgtggta gcgcacgatg
     5821 atatggtcga tgatttctgc cagcggggcg ctacgccagt ctttctcaat cggttgttca
     5881 gcaagctttg ccagctcagc ttcaatgacc tcaacatcca gttctttacg tgccgccgca
     5941 cgcgccagcg tttgcttacc gccgcagcag taatccatat catatttacg aaacagagcc
     6001 gaagcgcgag gaatagagag cgccagttca cctaaaggtt ggtcgcgata agccatagct
     6061 gatacctcat tcttaataag ataagatgta ttttaaatgc atctttaagg caaaagctat
     6121 aacccttacg tagcagaggc ttatttaact cactgccagc gtgtcaactt tctgagacag
     6181 attcagttaa aggtttttct ggttttgctc taaccgcgat gaccacgcca atcactaaca
     6241 gcgcgatacc gctcagcgtc attagcggcg gcatttgctg gcggagtaaa aaggtgtaca
     6301 gcaaacctgc cagcgtttcg aaaacaatca gcggcccgag aatcactgtc ggtaatcgct
     6361 ggctggcgac gttccagcag agtgcgccaa cccatgagca aagcacggct atcgcaatca
     6421 tcagactaat aaacaccagc ggacgggggc caaagggtag ggagaagtcc ggcgtttgcg
     6481 tattcagcca gtaacaagcg acgagatagc cgataagtga aaccggcagt gtgaccagcg
     6541 cctgcgctgt tgcccacatc atcggatgtt tgtcgggatt ttcccgcagc cagcgggcgt
     6601 tgcgcagggc ataccacgcc cagcagacca cggaaactaa cgccagcacg atgccagagg
     6661 tataacgtgc ccagtcaaaa ttggggagtc cgtggtttaa ctcagcaata ttcacacacg
     6721 ccaggccgat gccaatacaa atcagtgccg gggcgagttt tccccacgcg agtttgccgt
     6781 cgcgctggct ataaagcaga ttggcgaaga cgggaatcac caccggcagg gtgccgataa
     6841 tcatcgtgga aacaggcgca ccagtacgtt gaatggcact ggcaaggcag aagtaataga
     6901 tgaggttgcc catcatagtg agcatcaagg cggtaagcca gtcgcgacgc gccaactgac
     6961 gcagacgcac gcgtcccagc caggcaatgg gcagcgcaat taaccctaac gccagataac
     7021 gccccatcga ctgcaacatc gccgggtatt ccggcacgat caacggcccg acaaaaataa
     7081 gcccccacat caaccctgct aacagggcgt acagcacgcc gctaatcatt actggcatcc
     7141 attgatctgt cagaagaaag ttcaggaagc agacatgatt gctcccggca tcccgtcatt
     7201 atcagagagg atgcgggagg atgccacttt aattaactag ttattaacat taaaaagatg
     7261 gcttacgctt tcggctaacg ttgtcgttgg gtggccaatc aatttgctaa gtgttttgct
     7321 gtcatcaaac agaccgcctt tcgatgcgcc aacgtcagaa tccgccagca tatccgccag
     7381 tccgtcgggc agtccgacgc ttttcagtgc cgcggcgaaa tcggcttcgc tcagattttg
     7441 ataggtaacc tgtttgccgc tctgtttggt cagctctgcc gctaactgtg tcaacgtcca
     7501 ggcactatcg cccgccagtt cgtaaacctt gccttcgtga ccggcttcgc taatcacgcg
     7561 tgccgcagct gccgcataat ctgcccgcgt tgctgaggcg attttgccat cgcccgccgc
     7621 accgataaat acgccgtgtt ccagtgctgc cggggcgctg gcgaggtagt tttcggtgta
     7681 ccagccgttg cgcagtaggg tgtaaacgat gccagaatca gccagcattt tctccgtctc
     7741 gatgtgctca tcggcgaggc cgagcggaga tttatccgcg tgaagcaggc tggtataagc
     7801 gataaatttc acgccagccg tctttgcggc attaataaca ttacgatgct gcggggcacg
     7861 ttgacccact tcgctggaag agatcagcag tagtttttcc actccctgaa gtgcagatgt
     7921 cagtgcggct tcatcgccgt agtcagcctg acgcacggta atgccttgtg ctgtcagtgc
     7981 ctgggctttt gccggattac gaacgatagc cactatttgg ctggcaggaa ccgttttcat
     8041 caaggattca ataacatact gaccaagttg gccagtggca ccagtaatag cgatcatggg
     8101 aagtctccat cgttttgtct tgtgtttaaa tacgctaaca cctaaactta cttttagtaa
     8161 gtacgcacaa aaaggtaagt atgaaatgag tcaggttagc ctgtcgcaac aactgaaaga
     8221 gggtaatctc tttgcggaac agtgcccgtc gcgcgaggtg ttgaaacacg tcaccagccg
     8281 ttggggggtg ttgattctgg tggcgctacg cgaaggtact catcgcttta gcgacctgcg
     8341 gcgcaaaatc ggcggggtga gcgaaaagat gcttgcgcag tcgttacagg cgttagaaca
     8401 ggatggtttt cttaaccgta tcgcgtatcc ggtggtgccg ccgcatgttg aatacagcct
     8461 cacgccgctg ggcgagcagg tgagcgaaaa ggttgctgca ctggcggact ggattgagtt
     8521 gaatttgccc gaggtgttgg cggtgcggga tgaacgtgcg gcataactgc cggatgcgcg
     8581 gcctacggtg tatgacttgt agggcggata agatgcgcca gcatcgcatc cgccaataaa
     8641 tgccggatgc gattgatgat ttataggccg aaaaagaagt gttttactta ctcaaatcca
     8701 cctgataaat cgcaaacccg atatcatcgg tcgcgacttt attcatcgga tactgccctt
     8761 tctctttaat aaacgctgcg gctttatctg acggggaagt ttcgaaacgg atatccagtt
     8821 tcttatcggc agctatcggt gctaaacgcc agttgttatc tgccgccggg tgaatttccc
     8881 ccgcacgttt cgactcatca gcaatccacg ctgccagcac cgagcggttc tcatccggtg
     8941 aagcaaaagc gatatggctg tcgcccgtcc cggcaaattt gccgccgtaa gcgcgatagt
     9001 tattggtggc aacgaggaac atagcgttcg gatcaatcgg cttgccatta aaggtcaggt
     9061 tcttaatcct ttccgcattg gcattaatca tctggcactc gccgtcataa cgggcgggct
     9121 gggtaacatc aatctgataa ttcacaccat caataacatc aaagttataa gtgcggaaac
     9181 catcccagtt gatgagtgac tgtggtttcg tgctgttggg atcaatctgg ttaaactgtc
     9241 cggcggagca ctccagccac tctttcacct ctttaccgct ggctttcacc acaatcagcg
     9301 tattgggata gagataaaga tcggcggcat tacggaaggt caactggcct ttttccacct
     9361 ccacatagct tgccgggtca tttttgcgac caccgacttt aaacggtgcg gcagctgaaa
     9421 gcaccggcag ttttgccaga tccggatcgc cctgaatgta atgctcgaca tacgcttttt
     9481 gcgcgttgtt cactacttgc acggtcggat cgtcctgcac cagcgccaga tagctataca
     9541 tattgtcggc agatttaccg attggcttgc tgacgaactg gcgtgtggca tcgtgatcgg
     9601 ctttgagtgt ttctaccagc ttgctgtctt ccgccgcgag ggatttttta ttggcgatgt
     9661 cgtaaatcgg ccgtgcttcc gctttcgcct gcgtcacctg ccatttaccg ctgtcattac
     9721 tgagttgtaa gtcgaccacc ccaagatgat cgccccacat gcccggcatt accgccggaa
     9781 caccattcag cgtgcctttg gtgatatcag ccccttcgat atcagcaaaa tctttacccg
     9841 ggaaaacggc gtgagcatgg ccaaacataa tggcgttaac gcccggaatt tcactgaggt
     9901 aataaactga gttttccgcc attactttat acggatcggc agacagcccg gaatgcgcca
     9961 gaacgacaac gacatcggca cctttctcgc gcatttcagg ctcgtatttg cgcacggttt
    10021 cggtaatatc attcaccgtc acttttccgg ataaattagc tttatcccag cccatgattt
    10081 gcggcggcac gacgccaata tagccaatct tcagcgtctg tttttttccg tctttatcga
    10141 ccacttcggt gtctttaatt aaatacggtg taaacattgg ctgtttggtt ctggcgtcaa
    10201 tgacgttggc atttacataa gggaatttcg ctcctgccag cgcatttttc aggtaatcca
    10261 gaccgtagtt aaactcgtgg ttgccaagcg ttccgacggt atagtccagc gtatttaatg
    10321 ccttatagac cgggtgaata tcacctgctt ttaatccttt cgccgacatg taatcggcca
    10381 gcggactccc ctgaatcaaa tcgccgttat cgaccagtac gctgtttttc acttcattgc
    10441 gggcattgtt aatcaggctt gccgtacgta ccagtccgaa tttttccgtg gcggtgtctt
    10501 tgtaataatc gaaatccatc atgttgctat gcagatcagt ggtttccatg atacgcagat
    10561 cgaccgtcgc tgcattcaca ctggcggcaa tcagcgtggc caggagcgtt gcgctaaact
    10621 taatcatcag ggacatcctt ttatcatcgg gaatacgaaa gaaaagggag aataaacgtc
    10681 ttacttatag aacagtgaag aatgccacaa ttttacgctt tgaaaatgat gacactatca
    10741 cagttggcgc attcattaat gatagggtat aagtaaaaca ataagttaac accgctcaca
    10801 gagacgaggt ggagaaatgt tagatcaagt atgccagctt gcacggaatg caggcgatgc
    10861 cattatgcag gtctacgacg gggcgaaacc gatggacgtc gtcagcaaag cggacaattc
    10921 tccggtaacg gcagcggata ttgccgctca caccgttatc atggacggtt tacgtacgct
    10981 gacaccggat attccggtcc tttctgaaga agatcctccc ggttgggaag tccgtcagca
    11041 ctggcagcgt tactggctgg tagacccgct ggatggtacg aaagagttta ttaaacgtaa
    11101 tggcgaattc accgttaaca ttgcgctcat tgaccatggc aaaccgattt taggtgtggt
    11161 gtatgcgccg gtaatgaacg taatgtacag cgcggcagaa ggcaaggcct ggaaagaaga
    11221 gtgcggcgtg cgcaagcaga ttcaggtccg cgatgcgcgc ccgccgctgg tggtgatcag
    11281 ccgctctcat gcggatgcgg agctgaaaga gtatctgcaa cagcttggcg aacatcagac
    11341 cacgtccatc ggctcttcgc tgaagttctg cctggtggcg gaaggacagg cgcagctgta
    11401 cccgcgtttc ggaccaacga atatttggga caccgccgct ggacatgctg tggctgcagc
    11461 tgccggagcg cacgttcacg actggcaggg taaaccgctg gattacactc cgcgcgagtc
    11521 gttcctgaat ccggggttca gagtgtctat ttactaaatt cagatggcag aaacagtgta
    11581 tt
//
LOCUS       DBFIFI010000131        11532 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.131, whole genome shotgun sequence.
ACCESSION   DBFIFI010000131 DBFIFI010000000
VERSION     DBFIFI010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11532
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.131"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..421
                     /locus_tag="N7B39_003340"
     CDS             <1..421
                     /locus_tag="N7B39_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HGU2423261.1"
                     /translation="GALWLATNHYRDNALTYKAQRDKKARELEQANATITDMQVRQRD
                     VAALDAKYSRELADARAENETLRADVAAGRKRLRINATCSGTVREATGTSGVDNATRP
                     RLADTAERDYFILRERLMTMQKQLEGAQEYIRTQCLN"
     gene            598..1149
                     /locus_tag="N7B39_003341"
     CDS             598..1149
                     /locus_tag="N7B39_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HGU2423262.1"
                     /translation="MNYLTIVNGIDFRDLVFVTGNEPVTDTRKVAVAFGKEHKDVLRK
                     TRAVVQQCSKEFAERNFALCYENNELQNGKPQPIYRMTKDGWTMLVFGFTGKAAMTFK
                     EAYIQAFNWMAELIQQGLANLEAERNAVMLEYMKEKDVASMSGRLLNRWGRVKKPQLL
                     ARLDRLEQQGQIALPGFDKGISA"
     gene            1442..2236
                     /locus_tag="N7B39_003342"
     CDS             1442..2236
                     /locus_tag="N7B39_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HGU2423263.1"
                     /translation="MAKLDWKKLEQAFRREHAETGITLLDWCRKKKINYNTARTRIKM
                     GKIDHEIDHKTDHDISDEEPCNDAGSGDEKCAKNSEKNCANSAETKRIRGSRLLPPSN
                     AFSQRNTHAVRHRGYAKYLEADNLMDDASDMVLFDELVFTRARALSVTKALKGMFADL
                     EEATDVETRVALYDKILKAEQALDRNIARIESIERSLLTLDVLAETAPKLRADRERIN
                     AARDKLRAETDILTSQRRGVVTPVSDIVSSLHEMSNSGRLDDIPEE"
     gene            2217..3923
                     /locus_tag="N7B39_003343"
     CDS             2217..3923
                     /locus_tag="N7B39_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HGU2423264.1"
                     /translation="MTFRKNEPRCDEPSEMTEAEQRLFIMTKLSNPWWRLNHLYKIQN
                     EKGELVTFRMRPAQRQLFRSMHNKNIILKARQMGFSTAIDIYLLDQALFIPHLKCGIV
                     AQDKQAASEIFRTKIAVPFDHLPDWLRASFTIVERRSGASGGYILFGHGSSIQVATSF
                     RSGTVQRLHISEHGKICAKYPAKAKELRTGTLNAVSDECIIFDESTAEGVGGDFYEMS
                     NRAQEITASGLLLTAQDYKFHFYAWWQDPKYSARVPESGLKLSREKMTYFSAVEKAMN
                     ITLTDEQKQWYINKETEQREEMKQEFPSTPQEAFLTSGRRVFSAESTLQAESFCSPPM
                     IVYDIEPVTGAKTKAQSLREGNKNELQRTLMNYLLVWELPDPDEEYVCGADTAEGLEH
                     GDRSSLDVVKRSNGEQVAHWFGHLDAELFAHLISQVCRMYNNAFVGPERNNHGHAVIL
                     KLRELYPTRYIYNEQHLDQAYDDDTPRLGWLTTRQSKPVLTEGMKTLLNNGISGIRWS
                     GTLSEMNTYVYDAKGSMNAQEGCFDDQLMSYMIAQEMRARMPVRVKQKTDKRRTTHWM
                     AH"
     gene            3923..6067
                     /locus_tag="N7B39_003344"
     CDS             3923..6067
                     /locus_tag="N7B39_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HGU2423265.1"
                     /translation="MKNEINTTAMKNDHGSTPRFSQRQLLSLCSDIDSQPLWRDAANK
                     ACAYYDGDQLAPEVIQVLKDRGQPMTIHNLIAPTVDGVLGMEAKTRTDLIVMSDDPND
                     ETEKLAEAINAEFADACRLGNMNKARSDAYAEQIKAGLSWVEVRRNSDPFGPEFKVST
                     VSRNEVFWDWLSREADLSDCRWLMRRRWMDTDEAKATFPGMAQVIDYAIDDWRGFVDT
                     TVTEGQPSPLMSAWEEYQSWDRQQNEWLQRERRRVLLQVVYYRTFERLPVIELSNGRV
                     VAFDKNNLMQAVAVASGRVQVKVGRVSRIREAWFVGPHFIVDRPCSAPQGMFPLVPFW
                     GYRKDKTGEPYGLISRAIPAQDEVNFRRIKLTWLLQAKRVIMDEDATQLSDNDLMEQI
                     ERPDGIIKLNPVRKNQKSVADVFRVEQDFQVASQQFQVMQESEKLIQDTMGVYSAFLG
                     QESGATSGVAISNLVEQGATTLAEINDNYQFACQQVGRLLLAYLLDDLKKRRNHAVVI
                     NRDDRQRRQTIVLNAEGDNGELTNDISRLNTHIALAPVQQTPAFKAQLAQRMSEVIQG
                     LPPQVQAVVLDLWVNLLDVPQKQEFVERIRAALGTPKSPDEMTPEEQEVAAQQQALQQ
                     QQAELQMREMAGRVAKLEADAARAHAAAQRDNASAQREVALTQGQRYVDALNQAHTAE
                     IITGVQNMEQEQDVLQQQMLYTLQQRMNEMSL"
     gene            6225..7232
                     /locus_tag="N7B39_003345"
     CDS             6225..7232
                     /locus_tag="N7B39_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2423266.1"
                     /translation="MDFEFTGEETPEQLEKMLEGLGDVDIDSHAQDVVTEDTTEKHAD
                     EEAQTQTGDNNVAPTPDASVEQTQDVKEPEAKGVLTRDGKHVIPYEVLEAERSGKQRA
                     EQEAALLRGQIAEEKRRVELLTSQIHQAGMKPTPLPENEKISDEQIARIREMYPEIGD
                     AVASLIRKNNYLQSRVQQSAQQAEGNGGEDLSPVLDAMNAVPVLKTWQESDPDRFSVA
                     VSIDGKLQNDPAWKDKTLTERFAEVARRTQVAFGEVSESSADNKADKTDIRKTAEEKV
                     KTAEQEQAVPASPSDLGTTASVGTGDNFERLLGASHSEAEAIMRGMTNAEIDALLEKL
                     G"
     gene            7256..8470
                     /locus_tag="N7B39_003346"
     CDS             7256..8470
                     /locus_tag="N7B39_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N4-gp56 family major capsid protein"
                     /protein_id="HGU2423267.1"
                     /translation="MTTVTSAQANKLYQVALFTAANRNRSMVNILTEQQEAPKAVSPD
                     KKSTKQTSAGAPVVRITDLNKQAGDEVTFSIMHKLSKRPTMGDERVEGRGEDLSHADF
                     SLKINQGRHLVDAGGRMSQQRTKFNLASSARTLLGTYFNDLQDQCAIVHLAGARGDFV
                     ADDTILPTAEHPEFKKIMINDVLPPTHDRHFFGGDATSFEQIEAADIFSIGLVDNLSL
                     FIDEMAHPLQPVRLSGDELHGEDPYYVLYVTPRQWNDWYTSTSGKDWNQMMVRAVNRA
                     KGFNHPLFKGECAMWRNILVRKYAGMPIRFYQGSKVLVSENNLTATTKEVAAATNIDR
                     AMLLGAQALANAYGQKAGGHFNMVEKKTDMDNRTEIAISWINGLKKIRFPEKSGKMQD
                     HGVIAVDTAVKL"
     gene            8526..8915
                     /locus_tag="N7B39_003347"
     CDS             8526..8915
                     /locus_tag="N7B39_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2423268.1"
                     /translation="MAKTILAPSLSERVYTGTHGNESVAEGVFTVNAAEADSVIHLLS
                     LPVGIRINSLQLVSTGGLGTATVSIKSGEHALIDNSEAVSAKFARYVPVEPYTTQRDG
                     ELVTVTIKTAAATGTLNVLLRYTVVGY"
     gene            8965..9426
                     /locus_tag="N7B39_003348"
     CDS             8965..9426
                     /locus_tag="N7B39_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2423269.1"
                     /translation="MSEKIAVVYIGPKPVKKDTITGSRTLFPRLEPVHVDSAMAWQLL
                     GFPDVWVRHEELDDVLKKQQQNEQLRQAQQAQERVLAALAEAENSFVVSVNGQEVDLS
                     KLTSARLATLCEAEELDIHKDPKETAEAFRIRVREAFRRRVAETEQHGGTE"
     gene            9410..9973
                     /locus_tag="N7B39_003349"
     CDS             9410..9973
                     /locus_tag="N7B39_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2423270.1"
                     /translation="MAELSDFLPYVRRHISGPLNIMMTDALSMAAVAFSRQSLVCRRE
                     VTVVPVAGKEIVLPYDKDDEECVHIIRISDDNHELFVGRDVDISSGRSLRFACSPGEV
                     SVLYAVAPKAGRSQIPDELLTWPEEVAAGALERLFMQTGVSWSDPSRAQYFSVQFSEG
                     IRRAYRHTLATSPYSSYRNPVRRQRFY"
     gene            9973..10623
                     /locus_tag="N7B39_003350"
     CDS             9973..10623
                     /locus_tag="N7B39_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6682 family protein"
                     /protein_id="HGU2423271.1"
                     /translation="MTTITEIIGRVNTQLVDPMMVRWPLQELCDYYNDAVRAVILARP
                     DAGASLETISCVPGARQVLPDGVIQLLDVICLSDGSAVRPLSREVLDAQYPEWPTMKG
                     IPECFISNDLSPRVFWLFPAPDKEISIDAVVSRIPEAVYVLTQDDDTPVPLEEAYVNP
                     LVDWMLFRAFSKDAAGGAESGLAAQHYQSFVEQLGIKQGADSALSARKKVFNGGGV"
     gene            10620..>11532
                     /locus_tag="N7B39_003351"
     CDS             10620..>11532
                     /locus_tag="N7B39_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HGU2423272.1"
                     /translation="MSVVVSGTLKSPDGEAISGANITLTALTVSPDALSGTSASAVTR
                     EGGYYGMTMDPGEYAVSVTVKGKTAVYGRVRIEGTESTVTLNMLLRRSLVEVSIPGEL
                     LTDFRQIQNNVADDLATIRRLNEDTATKNAQATQSKESAAASAKSASDSAKTATSRAA
                     EAGQKATDATEAATRAVTAAGNAEESSTRAGESEKAAGADAEKARQHAEKARLAQESA
                     GEILKRAEAATVSAEEARRMAENARGPRGPQGETGPKGDVGPKGETGPVGPQGPAGPK
                     GERGDVGAQGAVGPAGPRGEKGEQGERG"
BASE COUNT         2978 a         2690 c         3305 g         2559 t
ORIGIN      
        1 tggtgcgctg tggctggcaa caaaccatta ccgtgacaac gcgctcacct acaaagcgca
       61 gcgcgataaa aaagccagag agctggaaca ggcgaatgcc accattactg acatgcaggt
      121 gcgccagcgt gatgttgctg cgctcgatgc aaaatactcg agggagttag ccgatgcgag
      181 agctgaaaat gaaactctgc gtgctgatgt tgccgctggt cgtaagcgcc tgcggatcaa
      241 cgccacctgc tccggtaccg tgcgtgaagc caccggcacc tccggcgtgg ataatgcaac
      301 caggccccga ctggcagaca ccgctgaacg ggattatttc atcctcagag aacggttgat
      361 gacaatgcag aagcagctgg aaggggcaca ggaatatatc cgcactcagt gcctgaatta
      421 acagagccag cttaatcgct ggctttttca tatctgaatt tcatcgcgca tctcacgcgc
      481 atatcacatc cccgagcctt tcagaaagtt gagcctgaga actgccgtat atggtggcga
      541 ccatctcggg gcggcttttc tgtgagacag gctcaatttt ctgaaaggta agacgctatg
      601 aactatctga cgattgttaa cggtattgat ttccgcgatc ttgtttttgt gacaggcaac
      661 gagccggtga cggatacccg aaaggttgcc gtagcatttg gcaaagaaca caaagatgta
      721 ttgcggaaaa caagagccgt tgttcaacaa tgctcaaaag aatttgcaga gcgcaatttt
      781 gcgctttgct atgaaaacaa tgagttacag aacggtaaac cccagcccat atatcgaatg
      841 acaaaagacg gttggacgat gttggtattt ggttttacgg ggaaagctgc tatgaccttt
      901 aaggaagcgt acattcaggc tttcaactgg atggctgagc ttattcagca aggtctggcg
      961 aatctggagg cggagcgcaa cgctgtaatg ctggagtaca tgaaagagaa agatgtcgcc
     1021 agtatgtcag gtcgtttgct caatcgctgg ggcagggtga agaagcctca gttgctggca
     1081 aggctggaca ggctggagca gcaggggcag attgcgttac ccggatttga taaaggcatt
     1141 tcagcctgac atagccatgc gccgtatcgt cgccgtattc ccgcattaac agagaccgca
     1201 gccaccttat ctgcgtgagt gtgcggggat aatcaaaaac gatacacacc gggttttctc
     1261 atttttcacg agatgggagc gatttcccgc gaagccgcct gtccggtgcg gtggtggaag
     1321 aaaccggata aaacaaccgc attgtgcaaa tatcgatcaa atatggtgct gctgtgtgaa
     1381 atctgaaaaa tcacagcggt cattatgcat cagtttttaa cacaggacgt cagaacgtga
     1441 catggcaaag ctggactgga aaaagctgga gcaggcattc cgacgcgaac atgccgaaac
     1501 gggaataaca ttactggact ggtgccggaa gaaaaagatt aattacaaca ccgccagaac
     1561 ccgtataaaa atgggcaaaa tcgatcatga aattgatcat aaaaccgatc atgacatctc
     1621 agatgaagaa ccctgcaatg acgcgggttc cggcgatgaa aaatgcgcaa aaaactctga
     1681 aaaaaactgc gcaaattcgg cagaaacgaa acggattcgt ggttcccgac ttttaccacc
     1741 ttcaaacgct ttttctcagc gaaacaccca cgccgtaaga caccgtggat atgcgaagta
     1801 tcttgaggca gataacctca tggatgatgc gtccgacatg gtgctgttcg atgaactggt
     1861 gttcacccgg gcccgcgcac tttcagtaac taaggcactt aaagggatgt tcgccgacct
     1921 ggaagaggca actgacgtgg aaacccgtgt tgctctttac gacaaaatac tcaaagctga
     1981 acaggccctt gaccggaata ttgcccgtat cgagtcaatt gaacgctcat tgctgacgct
     2041 ggacgtcctg gctgagacag caccaaaact tcgtgctgac cgggaaagaa tcaacgccgc
     2101 cagagataaa ctcagagctg aaaccgatat tctgaccagc cagcgtcggg gcgttgttac
     2161 gcctgtcagt gacatcgtgt catcgctgca tgaaatgagt aattcgggga gactggatga
     2221 cattccggaa gaatgaaccg cgatgtgatg agccgtcaga aatgaccgag gctgaacaac
     2281 gtctgttcat catgactaaa ctgagcaatc cctggtggcg gctcaatcat ctctacaaaa
     2341 tacagaacga aaaaggtgaa ctggtcacct tcagaatgcg accggcgcag cgccagttgt
     2401 tccggagcat gcacaataaa aatattatcc tgaaagcgcg ccagatggga ttttccacag
     2461 ccattgatat ttatcttctc gaccaggcat tattcattcc gcatctcaaa tgcgggatcg
     2521 tcgctcagga taaacaggct gccagtgaaa ttttccgcac aaaaattgct gtaccgtttg
     2581 atcatctccc tgactggctg agagcctcat tcaccatcgt tgaacgtcgt agcggtgcca
     2641 gcggtggcta tatcctgttt ggtcacggct cgagtatcca ggtggcaacc tcattccgtt
     2701 caggtacggt gcagcgcctg catatctcag agcacggcaa aatttgcgcg aaatatccgg
     2761 ctaaggcgaa agaactgcga accggtacgc ttaatgccgt ctctgatgaa tgcattattt
     2821 ttgatgagtc cactgctgaa ggcgtgggtg gtgattttta cgagatgagt aaccgagcac
     2881 aggagatcac tgcatcaggc ttattgctga cggcacagga ttataaattc catttttacg
     2941 cctggtggca ggatcctaaa tacagcgcca gagtgccgga aagcgggctg aagctgtcac
     3001 gggaaaaaat gacgtatttt tctgcggttg agaaggcaat gaacatcacg cttactgatg
     3061 aacagaagca gtggtacatc aataaggaaa ctgaacagcg tgaggaaatg aagcaggagt
     3121 ttccctcaac gccacaggag gcgtttctga cgtccggacg acgtgtgttc agtgccgaaa
     3181 gtacgttgca ggcagaatca ttctgttcgc caccgatgat tgtttatgac attgaacctg
     3241 ttacaggagc gaagactaaa gctcagtctc tgcgtgaagg aaataaaaac gagttgcagc
     3301 ggacgctgat gaattatctg ctggtatggg aactgccgga tccggatgaa gagtatgttt
     3361 gtggggcaga tactgccgaa gggctggagc acggagaccg ctcatcgctg gatgttgtca
     3421 aacgcagtaa tggcgagcag gtggctcact ggttcgggca tctcgatgct gaactttttg
     3481 ctcatctcat ttcgcaggtc tgtcgtatgt ataacaacgc gtttgtgggg ccggagcgta
     3541 ataatcacgg acatgcagtt atcctgaaac tccgggaact ctatccgaca cgttatatct
     3601 acaacgaaca gcatcttgac caggcatatg acgacgatac gccccgcctt ggctggctga
     3661 caacccgtca gagcaaacct gttctgaccg aaggaatgaa aacgcttctg aataatggaa
     3721 tatcagggat ccgctggtca ggcacattat cggaaatgaa cacctacgtt tatgacgcga
     3781 aaggctccat gaatgcacag gaaggctgct ttgatgatca gctcatgagc tacatgattg
     3841 cccaggagat gcgcgccaga atgccggtga gggtaaaaca gaaaacggat aaacgcagaa
     3901 ccacacactg gatggcacac tgatgaaaaa tgaaattaac accacagcga tgaaaaacga
     3961 tcatggatcc acgccgcgtt tttctcagcg tcagttactg tctctctgtt ctgatattga
     4021 cagtcagcct ctctggcgtg atgccgcaaa caaggcctgt gcgtattatg atggcgacca
     4081 gctggcaccg gaagttatcc aggtactgaa agatcgcggt cagcccatga ccatccataa
     4141 cctcattgcc cccacggtag atggtgtact gggaatggag gcaaaaacaa gaacggacct
     4201 gatagtgatg tcagacgatc cgaacgatga aacagagaaa ctggcagagg ccattaatgc
     4261 ggagtttgct gatgcgtgcc gtcttggcaa catgaataag gcccgctccg atgcctatgc
     4321 ggaacaaatc aaggcgggcc tcagttgggt ggaggtcaga cgaaacagtg atccgttcgg
     4381 gcctgaattt aaggtgtcta ctgtcagccg gaatgaggtt ttctgggact ggctgagccg
     4441 ggaggctgat ttaagtgact gccgctggct gatgcgtcgc cgctggatgg ataccgatga
     4501 ggcaaaagct acattcccgg gaatggctca ggttatcgat tatgccattg atgactggcg
     4561 tggttttgtc gataccacgg ttactgaagg ccagcccagt ccgttgatga gtgcatggga
     4621 agagtatcag tcatgggatc gacagcagaa cgaatggctt cagcgtgaac gccgtcgtgt
     4681 gctgcttcag gtggtttatt accgtacatt cgagcgtctt ccggtgattg aactcagtaa
     4741 tggacgggtg gtggcctttg ataaaaataa tctgatgcag gcggtagctg tggcatccgg
     4801 gcgggttcag gtgaaagtcg ggcgggtaag ccgtattcgt gaagcctggt ttgtcgggcc
     4861 acactttatt gtggatcgcc cctgtagtgc tccgcagggg atgtttccgc tggttccttt
     4921 ctggggatac cgaaaggata aaaccgggga gccatacggg ctaatttccc gcgccattcc
     4981 ggcacaggat gaggtgaatt ttcgtcgtat caagctgacc tggttgcttc aggccaaacg
     5041 cgtgattatg gacgaggatg ccacccagtt gtcagacaac gacctgatgg agcagatcga
     5101 acgtccggat ggcattatta aactgaatcc ggtccgaaaa aatcagaaaa gtgtcgcaga
     5161 tgtttttcgg gttgagcagg attttcaggt tgccagccag cagtttcagg tcatgcagga
     5221 atcggaaaaa cttatccagg ataccatggg agtgtattcc gcatttctcg ggcaggagtc
     5281 aggtgcgacg tcaggcgtgg ctatcagtaa cctggtggag cagggggcca caacccttgc
     5341 ggaaatcaac gataactacc agtttgcctg ccagcaggtg ggaagactgt tgctggctta
     5401 tcttctcgat gacctgaaaa aacgccgtaa tcatgcagtg gtgattaatc gcgatgatcg
     5461 ccagcgtcgc cagaccattg tcctcaatgc tgaaggtgat aatggtgaac tgaccaatga
     5521 tatttcaaga ttaaatacac atattgcgct ggcgcctgtt cagcagacac cggcgtttaa
     5581 ggcacagctt gcacagagaa tgtcagaggt tattcagggg ctgccgcctc aggtgcaggc
     5641 tgttgtgctc gacctgtggg ttaatcttct ggatgtgccg cagaaacagg agtttgttga
     5701 gcgtattcgt gctgcgctgg ggacgccaaa atcaccagat gaaatgacgc cggaagaaca
     5761 ggaagtagcg gcacaacaac aggcacttca gcaacaacag gcagaactcc agatgcgcga
     5821 gatggctggc agagtggcaa aactggaagc tgacgccgcc agggcacatg cagctgcaca
     5881 acgggataat gccagtgcac agcgggaagt cgccctgaca caggggcagc gttatgtgga
     5941 tgcgcttaac caggcacata cggcagaaat cattaccggc gtacagaata tggaacagga
     6001 gcaggacgtt cttcagcaac agatgctgta tacgttacaa cagcggatga atgaaatgtc
     6061 gctctgaaaa ctctggcttc aactgaaccc cgtcatcgta cggggttttt tgtttccgga
     6121 ggtaagcgtt ccgggagcgg tgcgcttatt cgcgggggca gcgataagcc ttatttactc
     6181 aaccattcgg atctgtccga taaacagacc atgcggagtt atttatggat tttgaattta
     6241 cgggtgaaga aaccccggaa caactggaaa aaatgctgga aggacttggg gatgtggata
     6301 ttgacagtca cgcacaggac gtcgtgacgg aagataccac ggaaaaacat gcggatgagg
     6361 aagcacagac tcagacgggc gataacaatg tggcaccgac gccggatgcc agtgtggagc
     6421 agacgcagga cgtgaaggag ccggaagcga agggggtgct cacccgcgac ggtaaacacg
     6481 tcattcccta tgaagtcctt gaggctgaac gttccggtaa gcaacgggcc gaacaggaag
     6541 ccgcacttct tcgtgggcag atagctgaag aaaaacgcag ggtggaactg ctgacgtctc
     6601 agatccacca ggccggtatg aagcccacac cgttaccgga aaacgaaaaa atttctgatg
     6661 agcagattgc ccgtatcagg gagatgtatc cggaaattgg tgacgcggtg gcttcgctca
     6721 tccgtaaaaa taactatctc cagtcccgtg ttcagcaatc agcacagcag gcagaaggta
     6781 atggtggtga ggatttatca ccggttcttg atgcgatgaa tgccgtgccg gtgctgaaaa
     6841 cgtggcagga gtccgatcca gatcgcttct cggttgctgt atccatcgac gggaagctcc
     6901 agaatgaccc cgcatggaaa gacaaaacgc tcactgaacg tttcgctgaa gtggcccgtc
     6961 gtacgcaggt tgctttcggt gaagtcagtg agtcgtctgc tgacaacaag gcagacaaaa
     7021 cggatatccg gaaaacggcg gaagagaaag tgaagaccgc tgaacaggag caggcagtac
     7081 ctgcttcccc gtcagattta ggcaccacgg cttccgtcgg aaccggtgat aattttgaac
     7141 ggttacttgg cgcttctcat tcagaggcag aggcgattat gcgcggtatg acgaatgctg
     7201 aaatagacgc gcttctggag aagctcgggt aacttactga aggagaactg aagtaatgac
     7261 gactgtaaca tcagcccagg cgaataagct gtatcaggtg gcgcttttta ccgctgccaa
     7321 ccgcaaccgc tcgatggtaa atatcctcac tgaacagcag gaagcgccaa aagcggtttc
     7381 gccggacaag aaaagcacga agcagaccag cgcgggtgcg ccggttgtcc gtatcacaga
     7441 ccttaacaaa caggccggtg atgaagtgac cttcagcatc atgcacaaac tctcaaaacg
     7501 tccgacgatg ggagatgagc gtgttgaagg tcgtggtgag gatctcagcc atgctgactt
     7561 ctccctgaaa atcaatcagg gacgtcacct ggtggatgca ggcggacgta tgagtcagca
     7621 gcgcacgaag tttaacctgg catcctcagc cagaacgctt ctggggacgt actttaatga
     7681 cctgcaggac cagtgtgcga tagtgcatct tgctggagct cgtggtgatt ttgttgctga
     7741 cgacactatt ctgccgacag cggagcaccc tgaattcaaa aaaatcatga tcaacgatgt
     7801 actgcctccg acacatgacc gtcacttttt tggcggtgat gcgacaagct ttgagcagat
     7861 tgaagcggca gatatttttt ctattggcct ggtggacaat ctctccctgt tcattgacga
     7921 aatggcgcat ccgttacagc cggttcgtct gtccggtgat gaacttcacg gagaagatcc
     7981 atattacgtc ctgtacgtca cgccgcgtca gtggaatgac tggtacacct cgacgtccgg
     8041 taaggactgg aaccagatga tggttcgtgc cgtgaaccgt gcaaaaggtt ttaatcatcc
     8101 gctgttcaaa ggtgaatgtg cgatgtggcg caatatcctg gttcgtaagt atgcgggtat
     8161 gccgatccgt ttctatcagg ggtcaaaggt tctggtatca gagaataacc tgacggcaac
     8221 cacgaaagag gtcgctgctg caaccaatat tgaccgcgcc atgttactgg gggctcaggc
     8281 gctggcaaat gcttacggtc agaaggcggg cggtcacttc aacatggttg agaagaaaac
     8341 ggatatggat aaccgtactg agatagcaat cagctggatc aacggtctga aaaaaatccg
     8401 tttccccgag aagagcggca agatgcagga tcacggcgtg attgccgttg atacagcagt
     8461 gaagctctga ttttttcctt tccctatgcc gggttttcgc ccggcttttt caggagtcat
     8521 taattatggc aaagactatc cttgccccgt cactgagtga acgggtctat acgggtacgc
     8581 acggtaatga gtcggtggca gaaggcgtat ttacggtgaa tgctgcggaa gcggacagtg
     8641 ttattcatct tctctcactg ccagtgggca tccgtatcaa ctcactccaa ctggtttcaa
     8701 cgggtggtct gggtactgca accgtcagca ttaagtccgg tgagcatgct ctcatcgata
     8761 acagcgaagc tgtttctgca aaatttgcca gatatgtgcc agtggagccg tacaccacac
     8821 agcgtgacgg ggagctggtt actgtcacca ttaagactgc cgctgcaacc ggcaccctga
     8881 atgttctgct gcgttatacc gtggtgggat actgattaaa accttccggc ccgcgtcatg
     8941 cgggcttttt atccggggaa ttatatgagt gagaaaattg ccgttgtcta tatcggccca
     9001 aaacccgtga aaaaggacac cattaccgga agtcgcacgc tgttcccacg tcttgagccg
     9061 gtgcatgttg acagcgcgat ggcctggcaa ctgctggggt ttccggatgt ctgggttcgt
     9121 catgaagagc ttgatgatgt tctgaaaaag caacaacaga atgagcagtt gcggcaggca
     9181 cagcaggcgc aggaaagagt gcttgctgcg ctggcagaag cggagaacag ttttgttgtt
     9241 tctgttaacg ggcaggaggt ggatttaagt aagctcacct cagcacggct ggcgacgctg
     9301 tgtgaggcag aagagctgga tattcacaaa gacccgaaag aaacggctga ggcattccgt
     9361 atccgggtgc gtgaggcatt tcgccgtcgt gttgcggaga ctgaacagca tggcggaact
     9421 gagtgatttt ttaccgtatg tccgtcgtca tatcagcggt ccactgaaca ttatgatgac
     9481 ggatgctctg tcaatggctg ccgtggcatt cagccgccag tcgttggtgt gccgtcggga
     9541 ggttactgtt gtaccagtag caggaaaaga aatcgtgctt ccgtatgaca aagatgatga
     9601 ggagtgcgtt catatcatcc gtatctctga cgataatcat gagctttttg tcggtcggga
     9661 tgtggatatc agctccggac gctccctgcg atttgcctgt tctcccggtg aggtgagcgt
     9721 gctttatgcc gtcgctccga aagccggacg cagccagata ccggatgaac tcctcacatg
     9781 gcctgaagaa gtggctgcgg gggcacttga gcggttgttc atgcagactg gtgtttcatg
     9841 gtcagatccg tcacgcgcac agtatttttc tgtgcagttt tctgagggga tccgtcgggc
     9901 atatcgtcat acactggcga caagcccgta ctcttcatac cgcaaccctg tacgcaggca
     9961 gaggttttac tgatgacgac gattacagaa atcatcggac gtgtgaatac acaactggtt
    10021 gacccgatga tggttcgctg gcccctgcag gaattgtgcg attattacaa tgatgctgtg
    10081 agggcagtga ttctggcgag accggatgct ggcgcaagcc tggaaacaat cagttgtgtt
    10141 cctggcgccc gtcaggtttt gcccgatggt gtaatacaac ttcttgacgt gatatgcctc
    10201 agtgacggta gtgcagtcag accattatcc cgggaggtgc tggatgcgca gtatcctgag
    10261 tggcccacaa tgaagggtat tcctgaatgt tttatcagca acgacctgtc cccgcgcgta
    10321 ttctggctgt ttcctgctcc tgacaaagag ataagtattg atgcagtggt aagccggata
    10381 ccggaggcag tgtatgttct gacgcaggac gatgatacgc cagttccact ggaagaggct
    10441 tatgttaacc cactggtgga ctggatgttg tttcgcgctt tcagtaagga tgctgccggt
    10501 ggcgcagaat cggggctggc tgcgcagcat tatcagagtt ttgttgagca actagggatc
    10561 aaacaggggg cagacagtgc attgtctgcc cgtaaaaaag tgtttaacgg aggtggagtg
    10621 tgagtgttgt tgtttcgggg acgctgaaat ctcctgatgg tgaggcgata tcaggagcaa
    10681 atattaccct gacggcgctg acagtttcac cggatgcgct cagcggcacc agtgcgtcgg
    10741 cagtgacccg tgaaggtgga tattacggaa tgacgatgga tccgggggag tatgcggttt
    10801 cggtgacggt gaaagggaag actgctgtct acggacgtgt gcgtattgag gggaccgaaa
    10861 gtacggtgac gctcaatatg ctgttacgcc gcagtcttgt tgaggttagc atacccggag
    10921 aactgctgac agatttccgg cagatacaga ataatgtggc tgatgacctt gccactattc
    10981 gtcgcctgaa tgaagacacg gcgacaaaaa acgctcaggc cacacagtca aaagaaagtg
    11041 cagcagccag tgcgaagagc gcatctgaca gtgcaaagac ggcaaccagc agggcggctg
    11101 aagccggaca aaaagcgact gatgccactg aggctgcgac ccgtgcagtc acagcagcgg
    11161 ggaatgcaga ggaaagctcg acccgtgccg gagagtctga aaaagccgcc ggagctgatg
    11221 cagaaaaagc cagacagcat gctgaaaagg ccaggctggc gcaggagagc gccggagaga
    11281 tccttaagcg ggcagaggct gccactgtca gtgctgaaga ggccagacgt atggctgaga
    11341 atgcacgggg gccccggggg cctcagggag aaactggtcc gaagggggat gtcggtccta
    11401 aaggcgaaac aggtccagtg ggccctcaag ggcccgcagg gccgaaaggt gagcgtggtg
    11461 acgttggtgc tcagggggct gtagggcccg ctggtccgcg tggtgagaag ggcgaacagg
    11521 gggagcgagg ac
//
LOCUS       DBFIFI010000132        11457 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.132, whole genome shotgun sequence.
ACCESSION   DBFIFI010000132 DBFIFI010000000
VERSION     DBFIFI010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.132"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(97..1464)
                     /gene="rhlE"
                     /locus_tag="N7B39_003352"
     CDS             complement(97..1464)
                     /gene="rhlE"
                     /locus_tag="N7B39_003352"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HGU2423273.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRK
                     PAAAQ"
     gene            1693..2364
                     /gene="cecR"
                     /locus_tag="N7B39_003353"
     CDS             1693..2364
                     /gene="cecR"
                     /locus_tag="N7B39_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HGU2423274.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            2367..3362
                     /gene="hlyD"
                     /locus_tag="N7B39_003354"
     CDS             2367..3362
                     /gene="hlyD"
                     /locus_tag="N7B39_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HGU2423275.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            3355..5091
                     /gene="ybhF"
                     /locus_tag="N7B39_003355"
     CDS             3355..5091
                     /gene="ybhF"
                     /locus_tag="N7B39_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGU2423276.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            5084..6217
                     /gene="ybhS"
                     /locus_tag="N7B39_003356"
     CDS             5084..6217
                     /gene="ybhS"
                     /locus_tag="N7B39_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU2423277.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            6228..7334
                     /gene="ybhR"
                     /locus_tag="N7B39_003357"
     CDS             6228..7334
                     /gene="ybhR"
                     /locus_tag="N7B39_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU2423278.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(7296..7706)
                     /gene="ybhQ"
                     /locus_tag="N7B39_003358"
     CDS             complement(7296..7706)
                     /gene="ybhQ"
                     /locus_tag="N7B39_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HGU2423279.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            7839..8600
                     /gene="ybhP"
                     /locus_tag="N7B39_003359"
     CDS             7839..8600
                     /gene="ybhP"
                     /locus_tag="N7B39_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HGU2423280.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNTSASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            8597..9838
                     /gene="clsB"
                     /locus_tag="N7B39_003360"
     CDS             8597..9838
                     /gene="clsB"
                     /locus_tag="N7B39_003360"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HGU2423281.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            9838..10794
                     /gene="ybhN"
                     /locus_tag="N7B39_003361"
     CDS             9838..10794
                     /gene="ybhN"
                     /locus_tag="N7B39_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HGU2423282.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHVPAGIGVLEAVFIALLAGEHTSKGT
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(10830..>11457)
                     /locus_tag="N7B39_003362"
     CDS             complement(10830..>11457)
                     /locus_tag="N7B39_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HGU2423283.1"
                     /translation="GLVATAGLAWGAKYLEITATKYDSPPMYVAIGLLLLCMYGLIKD
                     INKINAAIAGVIYLFLLSLVAIVVASLVPVYAIIVVFSTAGAMFLISMLAGLLFNIDP
                     GSHRFMIMMTLTGLALVIIVNAALMSERPIWVISCLMIVLWSGIILHGRNKLLELAGK
                     CHSEELWSPVRCAFTGALTLYYYFIGFFGILAAIAITLVWQRHTRFFH"
BASE COUNT         2643 a         3058 c         3106 g         2650 t
ORIGIN      
        1 tattaaaaca ataaaatcag acatgtcatg catgtctgac cgtgttacct gaataatggg
       61 caaaagcctg tgatactctt ttgcccttac atggtattac tgcgcagcgg caggtttacg
      121 cggacggcga cggcgttgtt gttcgcctgc cggtttggca tcgccaagac gacgagatgg
      181 tttttctgca ggcttcgcgc tagctgattt cgcaccacct tctccacggc gtggttgctg
      241 ttgaccgcga ccaccaccac cttgcccacg accgccgccg ccacgttgct ggcgaccgtt
      301 ctggatcggt tcggctttga ttgacgggtc cggctcataa cccggaatcg caatgcgcgg
      361 gatctctttt ttcagcagtt tttcgatatc acgcagcagt ttgtgttcat caacacacac
      421 cagcgacaat gcttcgccag ttgccgccgc acggccagta cgcccgatac ggtggacata
      481 atcttccggt acgtttggca gttcatagtt gacgacgtgc ggcaactctt cgatatccag
      541 accgcgcgca gcgatgtcag ttgccaccag tacacgaata tcgcccgatt taaaatcggc
      601 cagcgcacgg gtacgcgcac cttgcgattt attaccgtgg atcgccgcac tacggatgcc
      661 atctttatta agctgttcag cgagatggtt agcgccatgt ttggtacggg taaacaccag
      721 cacctgctgc cagttccctt tcccaatcat gtgcgacagc aattcgcgct tgcgtttctt
      781 atcaacaaag tgaacatgct gggtcacctg atcagacgcg gtattgcggc gtgccacttc
      841 tatttccagc gggttgtgca acagtttttc cgccagggct ttaatatcgt cagagaaggt
      901 cgcggagaac aacaggttct ggcgcttcgc aggtagtttt gttaacacgc gacggatatc
      961 gtggataaag cccatgtcga gcatgcggtc agcttcatcg aggacgagga tttcaacctg
     1021 atccagcttc actgcattct gatgttccag gtccagcaaa cgtcccggcg ttgccaccag
     1081 cacatcaacg ccgccgcgca acttcatcat ttgtgggtta atgctgacac cgccaaacac
     1141 caccagcgaa cgaatgttca ggtatttgct gtaatcacgg acgttttcgc caatctgcgc
     1201 cgccagttca cgggtcgggg taagaatgag cgcacgtacc ggacgacgcc ctttggcgtg
     1261 cggctggcga gtgatcaggt gttgcaacag cggcagcgta aagcccgctg ttttgccggt
     1321 gccggtctgg gcgctagcca tcaggtcgcg gccttccagc accgcaggga tcgcctgctg
     1381 ctgaataggg gtgggttcac ggtaaccctg ctcggcaacg gcgcgcagga tatcagggct
     1441 taaacccaaa gaatcgaaag acataactac tccgaaccgc cccgaccgtt accggtgtag
     1501 ttttcaggga gatactgaaa aggaaaatga caaaaaccac aatgtcatga gggagcggag
     1561 tgtagcagtt tttgtgacgc agcgcataaa ttatccctgt gactggcgta gctaaaattc
     1621 ttagccatac tggacaactc ccatatggag tcataatctt aatcaatcat ttgattaaga
     1681 atggactgtg cgatgaataa tcctgccatg acaatcaagg gtgaacaggc gaaaaaacag
     1741 ctgattgctg ccgcactggc gcagtttggt gaatatggaa tgaacgccac cacccgcgag
     1801 atagccgccc aggccgggca gaatatcgct gccatcacct actacttcgg ttcgaaagaa
     1861 gatttgtacc tcgcctgcgc ccagtggatt gccgatttta ttggcgagca gttccgtccg
     1921 catgccgagg aagccgaacg cttgttcgcc caaccacagc ctgatcgggc tgccatccgt
     1981 gaactgatcc ttcgcgcctg caggaacatg attaagctgc tcacccagga tgacaccgtc
     2041 aacctcagca agtttatctc ccgtgagcag ctctctccca cggcagccta ccacctggtg
     2101 cacgaacagg tgattagtcc gctacacagc cacctgacac gcctgattgc cgcctggacc
     2161 ggctgcgacg ccaatgacac ccgcatgatc cttcataccc atgcgttgat tggtgagatt
     2221 ctggcgttcc gtcttggcaa agaaacaatt ctgttacgta ccggctggac cgcgttcgat
     2281 gaagaaaaga ccgaactgat caaccagacg gtgacctgtc atatcgacct gattttgcaa
     2341 ggattatcgc aaaggagttt gtagtgatga aaaaacctgt cgtgatcgga ctggcggtag
     2401 tggtacttgc cgccgtggtt gccggaggct actggtggta tcaaagccgc caggataacg
     2461 gcctgacgct gtatggcaac gtggatattc gtacggtgaa tcttagtttc cgtgttgggg
     2521 ggcgcgttga atcgctggcg gtggacgaag gtgatgctat caaagcgggc caggtgctgg
     2581 gcgaactgga tcacaagccg tatgagattg ccctgatgca ggcgaaagcg ggcgtctcgg
     2641 tggcacaggc gcagtatgac ctgatgcttg ccgggtatcg cgatgaagaa atcgctcagg
     2701 ccgccgcagc ggtgaaacag gcgcaagccg cctatgacta tgcgcagaac ttctataacc
     2761 gccagcaagg actgtggaaa agccgcacca tttcggcaaa tgacctggaa aatgcccgct
     2821 cctcgcgcga ccaggcacag gcaacgctga aatcagcaca ggataaattg cgtcagtacc
     2881 gttccggtaa ccgtgaacag gacatcgctc aggcgaaagc cagcctcgaa caggcgcagg
     2941 cgcaactggc gcaggcggag ctgaatttac aggactcaac gttgatagca ccgtctgatg
     3001 gcacgctgtt aacgcgcgcg gtggagccag gcacggttct caatgaaggt ggcacggtgt
     3061 ttaccgtttc actaacgcgt ccggtgtggg tgcgtgctta tgttgatgaa cggaatcttg
     3121 accaggccca gccggggcgc aaagtgctgc tttataccga tggtcgcccg gacaagccct
     3181 atcacgggca gattggtttc gtttcgccga ctgctgaatt taccccgaaa accgtcgaaa
     3241 caccggatct gcgtaccgac ctcgtctatc gcctgcgtat tgtggtgacc gacgccgatg
     3301 atgcgttacg ccagggaatg ccagtgacgg tacaattcgg tgacgaggca ggacatgaat
     3361 gatgccgtta tcacgctgaa cggcctggaa aaacgctttc cgggcatgga caagcccgcc
     3421 gtcgcgccgc tcgattgcac cattcatgcc ggttatgtga cggggctggt agggccagac
     3481 ggtgcgggta aaaccacgct gatgcggatg ctggcgggat tactgaaacc cgacagcggc
     3541 aatgccacgg tgattggctt tgatccgatc aaaaacgacg gcgcgctgca cgccgtgctc
     3601 ggctatatgc cgcagaaatt cggcctgtat gaagatctga cggtgatgga gaacctcaat
     3661 ctgtacgcgg atttgcgcag cgtcaccggc gaggcacgga agcagacttt tgctcgcctg
     3721 ctggagttta cgtctcttgg cccgtttacc ggacgcctgg cgggcaagct ctccggtggg
     3781 atgaaacaaa aactcggcct ggcctgtacc ctggtgggcg aaccgaaagt gttgctgctc
     3841 gatgaacccg gcgtcggcgt tgaccctatc tcacggcgcg aactgtggca gatggtgcat
     3901 gagctggcgg gcgaagggat gttaatcctc tggagtacct cgtatctcga cgaagccgag
     3961 cagtgccgtg acgtgttact gatgaacgaa ggcgagttgc tgtatcaggg agaaccaaaa
     4021 gccctgacac aaaccatggc cggacgcagc tttctgatga ccagtccaca cgagggcaac
     4081 cgcaaactgt tgcaacgcgc cttgaaactg ccgcaggtca gcgacggcat gattcagggg
     4141 aaatcggtac gtctgatcct caaaaaagag gccacaccag acgatattcg ccatgccgac
     4201 gggatgccgg aaatcaacat caacgaaact acgccgcgtt ttgaagatgc gtttattgat
     4261 ttgctgggcg gtgccggaac ctcagaatcg ccgctgggtg caatattgca tacggtagaa
     4321 ggcacacccg gcgagacggt gatcgaagcg aaagaactga ccaagaaatt tggtgatttt
     4381 gccgccaccg atcacgtcaa ctttgccgtt aaacgcgggg agatttttgg tttgctgggg
     4441 ccaaacggcg cgggcaaatc gaccaccttt aagatgatgt gcggtttgct ggtgccgact
     4501 tccggccagg cgctggtgct ggggatggat ctgaaagaga gttccggtaa agcgcgccag
     4561 catctcggct atatggcgca aaaattttcg ctctacggca acctgacggt cgaacagaat
     4621 ttacgctttt tctctggtgt gtatggctta cgcggtcggg cgcagaacga aaaaatctcc
     4681 cgtatgagcg aggcatttgg cctgaaaagt attgcctcac acgccaccga tgaactgcca
     4741 ttaggtttta aacagcggct ggcgctggcc tgttcgctga tgcatgaacc ggatattctg
     4801 tttctcgacg aaccgacttc cggcgttgac cccctcaccc gccgtgaatt ctggctgcac
     4861 atcaacagca tggtagagaa aggcgtcacg gtgatggtca ccacccactt tatggatgaa
     4921 gcggaatatt gcgaccgcat cggcctggtg taccgcggga aattaatcgc cagcggcacg
     4981 ccggacgatt tgaaagcaca gtcggctaac gatgagcaac ccgatcccac catggagcaa
     5041 gcctttattc agttgatcca cgactgggat aaggagcata gcaatgagta acccgatcct
     5101 ttcctggcgt cgcgtgcggg cgctgtgcgt caaagagacg cggcagatcg ttcgcgatcc
     5161 gagtagctgg ctgattgcgg tagtgatccc gctgttgctg ctgtttattt ttggttatgg
     5221 cattaacctc gactccagca agctgcgggt cgggatttta ctggaacagc gtagcgaagc
     5281 agcgctggat ttcacccaca ccatgaccgg ttcgccctac atcgacgcca ccatcagcga
     5341 taaccgtcag gaactgatcg ccaaaatgca ggcggggaaa attcgcggtc tggtggttat
     5401 tcccgtggat ttcgccgaac agatggagcg cgccaacgct accgcaccga ttcaggtgat
     5461 caccgacggc agtgagccga ataccgctaa ctttgtgcag ggatatgtcg aagggatctg
     5521 gcagatctgg caaatgcagc gggcggagga taacgggcag acctttgagc cgcttattga
     5581 tgtacaaacc cgctactggt ttaacccggc ggcgattagc cagcacttta ttatccccgg
     5641 tgcggtgacc atcatcatga cggtcatcgg cgcgattctc acctcgctgg tggtggcgcg
     5701 agaatgggaa cgcggcacta tggaggctct gctctctacg gagattaccc gcacggaact
     5761 gctgctgtgt aagctgatcc cttattactt tctcgggatg ctggcgatgt tgctgtgtat
     5821 gctggtgtca gtgtttattc tcggcgtgcc gtatcgcggt tcgctgctga ttctgttttt
     5881 tatctccagc ctgtttttac tcagtaccct ggggatgggg ctgctgattt ccacgattac
     5941 ccgcaaccag ttcaatgccg ctcaggtcgc cctgaacgcc gcgttcctgc cgtcgattat
     6001 gctttccggc tttatttttc agatcgacag tatgcccgcg gtgatccgcg cggtgacgta
     6061 cattattccc gcacgttatt tcgtcagcac cctgcaaagc ctgttcctcg ccgggaatat
     6121 tccagtggtg ctggtggtga acgtactgtt tttgatcgct tcggcggtga tgtttatcgg
     6181 cctgacgtgg ctgaaaacca aacgtcggct ggattaggga gaagagcatg tttcatcgct
     6241 tatggacgtt aatccgcaaa gagttgcagt cgttgctgcg cgaaccacaa acccgcgcga
     6301 ttctgatttt accggtgctg attcaggtga ttctgttccc gttcgccgcc acgctggaag
     6361 tgaccaacgc caccatcgcc atctacgatg aagataacgg cgagcattcg gtggagctga
     6421 cccaacgttt tgcccgcgcc agcgccttta cacatgtgct gctgctgaaa agcccacagg
     6481 agatccgccc gaccatcgac acacaaaagg cgttactact ggtgcgtttc ccggctgact
     6541 tctcgcgcaa actggatacc ttccagaccg cgccgttgca gttgatcctc gacgggcgta
     6601 actccaacag tgcgcaaatt gccgccaact acctgcaaca gatcgtcaaa aattatcagc
     6661 aggagctgct ggaaggaaaa ccgaaaccta acaacagcga gctggtggta cgcaactggt
     6721 ataacccgaa tctcgactac aaatggtttg tggtgccgtc gctgatcgcc atgatcacca
     6781 ctattggcgt aatgatcgtc acttcacttt ccgtcgcccg cgaacgtgaa caaggtacgc
     6841 tcgatcagct actggtttcg ccgctcacca cctggcagat cttcatcggc aaagccgtac
     6901 cggcgttaat tgtcgccacc ttccaggcca ccattgtgct ggcgattggt atctgggcgt
     6961 atcaaatccc cttcgccgga tcgctggcgc tgttctactt tacgatggtg atttatggtt
     7021 tatcgctggt gggattcggt ctgttgattt catcactctg ttcaacacaa cagcaggcgt
     7081 ttatcggcgt gtttgtcttt atgatgcccg ccattctcct ttccggttac gtttcgccgg
     7141 tggaaaacat gccagtatgg ctgcaaaacc tgacgtggat taatcctatt cgccacttta
     7201 cggacattac caagcagatt tatttgaagg atgcgagtct ggatattgtg tggaatagtt
     7261 tgtggccgct actggtgata acggccacga cagggtcagc ggcgtacgcg atgtttagac
     7321 gtaaggtgat gtaacttctt atctttcgcc agcaaagaca ctaccgccgg gccggcaagg
     7381 attgccagcc ctgcaattgc cagcaaaaag ttgttttcca gtacgcggga caacagccac
     7441 agcacaatca gcgctgcgcc aaaataccag gtcgtggtca gctcttccag cacgtctgcc
     7501 acctcacgcc agcgttgctc ggggtggcgc tgaaacacca gcatcatcac taccgtgaca
     7561 caatacagtg cgcataatcc cacgccgacg tgaaccagag tcttactcat ccagcccacc
     7621 accagcagcg ccactaccag taaatgcaac ataatctgcc agcaacttag acccgttgcg
     7681 acacgaacac gttgttgcca cttcatggct tctctcctga agaatttatc tctacttact
     7741 cagagtaccg aactttacgg aaaattccgt aagaccgcac ctttttgtga cgccgactac
     7801 actatttcaa tgagataaga ggcaaagaca ggaaaattat gcccgatcaa acacaacaat
     7861 tttcgttcaa ggtgctcacc atcaatattc acaaaggctt taccgcgttt aaccgacgct
     7921 tcattttgcc ggaacttcgc gatgccgtgc gtaccgtcag cgccgatatt gtttgcctgc
     7981 aggaagtgat gggcgcgcac gaagttcatc cgctgcatgt ggaaaactgg cccgatacct
     8041 cgcactacga gtttctcgcc gacactatgt ggagcgattt tgcctacggt cgcaatgccg
     8101 tatacccgga agggcatcac ggcaacgccg tactgtcgcg ttatcccatt gaacattatg
     8161 agaatcgcga tgtttcggtc gatggtgcgg aaaagcgcgg cgtgctctac tgccgcattg
     8221 tgccgccgat gaccggaaaa gcgattcatg tgatgtgcgt acatctgggc ctgcgtgagg
     8281 cgcaccgtca ggcgcagctt gcgatgctcg ccgaatgggt gaatgagcta ccggacggcg
     8341 aaccggtatt ggtggcgggt gatttcaatg actggcggca aaaagctaat catccgttaa
     8401 aagtgcaggc cggactggat gagattttta cccgcgccca cggacgcccg gcgcgcacgt
     8461 ttccggtgca atttcctcta ctacgactgg acaggatcta cgtcaaaaat accagcgcca
     8521 gcgcgccaac cgcgttgccg ctgcggacat ggcgacacct ttctgatcat gcccctttaa
     8581 gtgcggagat tcatttatga aatgtagctg gcgcgaaggc aataagatcc agttgctgga
     8641 aaacggcgag caatattatc ccgcggtgtt taaggcgatt ggcgaggcac aagaacgcat
     8701 cattcttgaa acgtttatct ggtttgagga tgacgtcggc aaacaactgc atgcggcact
     8761 actggcagca gcgcaacgcg gggttaaagc ggaagtcttg ctggatggct acggttcgcc
     8821 ggatctcagc gatgagtttg tcaatgaact gacggcagct ggcgtagtgt tccgctacta
     8881 cgatccccgc cctcgccttt ttggtatgcg caccaatgtg tttcgccgga tgcatcgcaa
     8941 aattgtggta atcgacgcgc gtatcgcctt tattggcggg ctgaattact ccgccgagca
     9001 tatgtccagc tacggtccag aggctaaaca ggattacgcg gtacgccttg aagggccgat
     9061 tgtcgaagat attctccagt ttgagctgga aaacctgcct ggacagagcg cggcacgacg
     9121 ctggtggcga cgtcatcaca aagcggaaga gaaccgccag ccgggagaag cgcaggtatt
     9181 gctggtctgg cgcgataacg aagaacatcg cgatgatatt gaacgtcact atctgaaaat
     9241 gctcactcag gcgcggcgag aagtcattat cgccaacgcc tacttcttcc ccggctatcg
     9301 atttttacac gccttgcgta aagcggcacg gcgcggggtg cggatcaaac tgatcattca
     9361 gggcgaaccg gatatgccga ttgtcagagt cggtgcgcgc ttgctgtata actatctggt
     9421 taaaggcggc gttcaggtgt ttgagtaccg ccgccgcccg ctccacggca aagtggcatt
     9481 gatggacgat cactgggcga cagtagggtc cagtaatctc gatccgctca gtttgtcact
     9541 gaatctcgaa gcaaatgtca tcatccacga tcgtcatttt aaccagacgc tgcgcgataa
     9601 tctgaacggc attattgccg cagattgtca gcaggtcgat gagaccatgc tgccgaaacg
     9661 cacctggtgg aacctaacca aaagcgtgct ggcgttccac tttttacgcc acttcccggc
     9721 gctggttggc tggcttccgg cacacacgcc acgtctggcg caggttgatc ctcccgcaca
     9781 accgacaatg gaaacgcagg atcgggtaga aactgaaaac acgggggtaa aaccctgatg
     9841 agtaaatcac acccgcgctg gcgcttagca aagaagatcc tcacctggct gttttttatc
     9901 gcggtgattg tgttgctggt ggtctacgcc aaaaaagtgg actgggaaga ggtctggaag
     9961 gtcatccgcg actacaatcg cgttgcgctg cttagtgcgg tcgggctggt ggtcgtcagc
    10021 tatctgattt acggctgcta tgacctgctc gcccgctttt actgcggtca caaactggcg
    10081 aagcgccagg tgatgctggt gtcgtttatc tgctacgcct tcaacctgac gctcagtacc
    10141 tgggtcggcg gcattggtat gcgctatcgt ttgtactccc gtctgggatt gccgggcagc
    10201 actattacgc ggattttctc gctcagtatt accaccaact ggctgggcta tattttactg
    10261 gcagggatta tctttaccgc aggcgtggtg gagttgccgg accactggta tgtcgatcaa
    10321 actacgctgc gtattctcgg cattggctta ctgatgatta tcgcggttta tttgtggttt
    10381 tgcgctttcg cgaagcaccg ccatatgacc atcaaagggc aaaagctggt gctgccttcg
    10441 tggaaattcg ccctcgccca gatgctgatt tccagcgtta actggatggt catgggggcg
    10501 attatctggc tgttacttgg tcaaagcgtg aactatttct ttgtactggg cgtgttgctg
    10561 gttagcagta ttgccggcgt catcgtgcat gttccggcgg ggattggtgt gctggaagcg
    10621 gtgtttatcg cgctactggc tggggagcat acatccaaag gcacaattat cgccgcccta
    10681 ctcgcttacc gtgtgctgta ttactttatc ccgctgctgc tggcgctgat ctgttacctg
    10741 ttgctggaaa gccaggcgaa gaagctacgg gcgaaaaatg aagcggcgat gtgaagttta
    10801 gttaagttct ttagtatgtg catttacgac taatgaaaaa aacgcgtatg cctttgccag
    10861 acaagcgtta tagctatcgc ggcaagtatc ccaaagaagc cgataaaata gtaatagagt
    10921 gttaatgcac ctgtaaaagc gcaacgaacc ggactccaca actcttcact atggcatttc
    10981 cccgccaatt caaggagctt atttcgtcca tgcaagataa tgcctgacca taacacaatc
    11041 attaagcaac ttattaccca aatgggccgt tcactcatta atgcggcatt cacgatgatt
    11101 accagggcca accctgtcaa cgtcatcata atcataaaac ggtgagaacc aggatcaata
    11161 ttaaataata aaccggccag catactgatt aaaaacatcg cgcccgcagt gctgaacacg
    11221 acgataatgg catatacagg aactaaactt gcaacgacaa tcgccaccaa agagagtaaa
    11281 aacagatata ttacgcctgc gatggcggca tttatcttgt tgatatcctt aattaagcca
    11341 tacatacaaa gtaataataa ccctatggcg acatacattg gtggtgaatc atatttggtt
    11401 gccgtaattt cgagatactt agccccccag gccagccctg cggttgccac caaacct
//
LOCUS       DBFIFI010000133        11437 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.133, whole genome shotgun sequence.
ACCESSION   DBFIFI010000133 DBFIFI010000000
VERSION     DBFIFI010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.133"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            67..2490
                     /gene="pgaA"
                     /locus_tag="N7B39_003363"
     CDS             67..2490
                     /gene="pgaA"
                     /locus_tag="N7B39_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901515 -
                     poly-beta-1,6-N-acetyl-D-glucosamine transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HGU2423284.1"
                     /translation="MYSSSRKRCPKTKWALKLLTAAFLAASPAAKSAVNNAYDALIIE
                     ARKGNTQPALSWFALKSALSNNQIADWLQIALWAGQDKQVITVYNRYRHQQLPARGYA
                     AVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLNS
                     GAPDKANLLAEAYIYKLAGRHQDELRAMTESLPENASTQQYPTEYVQALRNNQLAAAI
                     DDANLTPDIRADIHAELVRLSFMPTRSESERYAIADRALAQYAALEILWHDNPDRTAQ
                     YQRIQVDHLGALLTRDRYKDVISHYQRLKKTGQIIPPWGQYWVASAYLKDHQPKKAQS
                     IMTELFYHKETIAPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTP
                     TSIPNDTWLQGHSFLSTVAKYSNDLPQAEMTARELAYNAPGNQGLRIDYASVLQARGW
                     PRAAENELKKAEVIEPRNINLEVEQAWTALTLQEWQQAAVLTHDVVEREPQDPGVVRL
                     KRAVDVHNLAELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLKDNWRGFAGFGYADG
                     QFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERVFNHEHKPGARLSGWYDFNDNWRIGS
                     QLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRHEVSLE
                     GQERIWSSPYLIVDFLPSLYYEQNTEHDTPYYNPIKTFDIVPAFEASHLLWRSYENSW
                     EQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLY
                     VEFDMTFRF"
     gene            2499..4517
                     /gene="pgaB"
                     /locus_tag="N7B39_003364"
     CDS             2499..4517
                     /gene="pgaB"
                     /locus_tag="N7B39_003364"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HGU2423285.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRKSLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADKQVKFGDELVD
                     REYFATWQQVREVARSRLVELASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKVEVNPHVFIWPYGEANGIAIEELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQQIITVQEKSPQRIMHIDLDYVYDENLQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYHRLSPFDDRVRAQVGMLY
                     EDLAGHAAFDGILFHDDALLSDYEDASAPAITAYQQAGFSGSLSEIRQNPEQFKQWAR
                     FKSRALTDFTLELSARVKAIRGPHIKTARNIFALPVIQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYLEGVAEKSADQWLIQLTNQIKNIPQAKDKSILELQAQNWQKNGQHQAISS
                     QQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDLIRPEFSTAWYPKND"
     gene            4510..5835
                     /gene="pgaC"
                     /locus_tag="N7B39_003365"
     CDS             4510..5835
                     /gene="pgaC"
                     /locus_tag="N7B39_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008375 - acetylglucosaminyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042710 - biofilm formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine synthase"
                     /protein_id="HGU2423286.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIVGGVYFWVYRERRWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYSLTTIWAF
                     TCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEHNLT
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            5837..6250
                     /gene="pgaD"
                     /locus_tag="N7B39_003366"
     CDS             5837..6250
                     /gene="pgaD"
                     /locus_tag="N7B39_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HGU2423287.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA"
     gene            complement(6300..7088)
                     /gene="phoH"
                     /locus_tag="N7B39_003367"
     CDS             complement(6300..7088)
                     /gene="phoH"
                     /locus_tag="N7B39_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HGU2423288.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(7708..8979)
                     /gene="efeB"
                     /locus_tag="N7B39_003368"
     CDS             complement(7708..8979)
                     /gene="efeB"
                     /locus_tag="N7B39_003368"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HGU2423289.1"
                     /translation="MQYEDKNGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPVARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKGIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            complement(8985..10112)
                     /gene="efeO"
                     /locus_tag="N7B39_003369"
     CDS             complement(8985..10112)
                     /gene="efeO"
                     /locus_tag="N7B39_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HGU2423290.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(10170..11000)
                     /gene="efeU"
                     /locus_tag="N7B39_003370"
     CDS             complement(10170..11000)
                     /gene="efeU"
                     /locus_tag="N7B39_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HGU2423291.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
BASE COUNT         3009 a         2813 c         2692 g         2923 t
ORIGIN      
        1 ctttcttttc agttacctgt aattagatac agagagagat tttggcaata catggagtaa
       61 tacaggatgt attcaagtag cagaaaaagg tgcccgaaaa ccaaatgggc tttgaaactt
      121 cttactgccg catttttagc agcgagtccc gcggcgaaga gtgctgttaa taacgcctat
      181 gatgcattga ttattgaagc tcgcaagggt aatactcagc cagctttgtc atggtttgca
      241 ctaaaatcag cactcagcaa taaccaaatt gctgactggt tacagattgc cttatgggcc
      301 gggcaagata aacaggttat taccgtttac aaccgctacc gtcatcagca attaccagcg
      361 cgtggttatg cagctgtcgc cgtcgcttat cgtaacctgc aacaatggca aaactcgctt
      421 acactgtggc aaaaggcgct ctctctggag ccgcaaaata aggattatca acggggacaa
      481 attttaaccc tggcagatgc tggtcactat gatactgcgc tggttaaact taagcagctt
      541 aactctggag caccggacaa agccaattta ctcgcagaag cctatatcta taaactggcg
      601 gggcgtcatc aggatgaatt acgggcgatg acagagtcat tacctgaaaa tgcatctacg
      661 caacaatatc ccacagaata cgtgcaggca ttacgtaata atcaacttgc tgccgcgatt
      721 gacgatgcca atttaacgcc agatattcgc gctgatattc atgccgaact ggtcagactg
      781 tcgtttatgc ctacgcgcag tgaaagtgaa cgttatgcca ttgccgatcg cgccctcgcc
      841 caatacgctg cattagaaat tctgtggcac gataacccag accgtactgc ccagtaccag
      901 cgtattcagg ttgatcatct tggcgcgtta ttaactcgcg atcgttataa agacgttatt
      961 tctcactatc agcgattaaa aaagacgggg caaattattc cgccctgggg gcaatattgg
     1021 gttgcatcgg cttatctcaa agatcatcag ccgaaaaaag cacagtcaat aatgaccgag
     1081 ctcttttatc acaaggagac cattgccccg gatttatccg atgaagaact tgcggatctc
     1141 ttttacagcc acctggagag tgaaaattat ccgggcgcgc taactgtcac ccaacatacc
     1201 attaatactt cgccgccttt ccttcggtta atgggcacgc ctacgagcat cccgaatgat
     1261 acctggttac aggggcattc gtttctctca accgtagcaa aatatagtaa tgatcttcct
     1321 caggctgaaa tgacagccag agagcttgct tataacgcac caggaaatca gggactgcgc
     1381 attgattacg cgagtgtgtt acaagcccgc ggttggcctc gtgcagcaga aaatgaatta
     1441 aaaaaagcag aagtgatcga gccacgtaat attaatctgg aggttgaaca agcctggaca
     1501 gcattaacgt tacaagaatg gcagcaggca gctgtcttaa cgcacgatgt tgtcgaacgt
     1561 gaaccgcaag atcccggcgt tgtacgatta aaacgtgcgg ttgatgtaca taatcttgca
     1621 gagcttcgta tcgctggctc aacaggaatt gatgccgaag gcccggatag tggtaaacat
     1681 gatgtcgact taaccaccat cgtttattca ccaccgctga aggataactg gcgcggtttt
     1741 gctggattcg gttatgccga tggacaattt agcgaaggaa aagggattgt tcgcgactgg
     1801 cttgcgggtg ttgagtggcg gtcacgtaat atctggctcg aggcagagta cgctgaacgc
     1861 gttttcaatc atgagcataa acccggcgcg cgcctgtctg gctggtatga ttttaatgat
     1921 aactggcgta ttggttcgca actggaacgc ctctctcacc gcgttccatt acgggcaatg
     1981 aaaaatggtg ttacaggcaa cagtgctcag gcttatgttc gctggtatca aaatgagcgg
     2041 cgtaagtacg gtgtctcctg ggctttcact gatttttccg acagtaacca gcgtcatgaa
     2101 gtctcacttg agggtcagga acgcatctgg tcttcaccat atttgattgt cgatttccta
     2161 cccagtctgt attacgaaca aaatacagaa cacgataccc catactacaa ccctataaaa
     2221 acgttcgata ttgttccggc atttgaggca agccatttgt tatggcgaag ctatgaaaat
     2281 agctgggagc aaatattcag cgcaggtgtt ggtgcctcct ggcaaaaaca ttatggcacg
     2341 gatgtcgtca cccaactcgg ctacgggcaa cgcattagct ggaatgacgt gattgatgct
     2401 ggcgcaacgc tacgctggga aaaacgacct tatgacggtg acagagaaca caacttatac
     2461 gttgaattcg atatgacatt cagattttaa ggataaatat gttacgtaat ggaaataaat
     2521 atctcctgat gctggtgagt ataattatgc tcaccgcgtg cattagccag tcaagaacat
     2581 catttatacc gccacaggat cgcaaatctt tactcgccga gcaaccgtgg ccgcataatg
     2641 gttttgtagc gatttcatgg cataacgttg aagacgaagc tgccgaccag cgttttatgt
     2701 cagtgcggac atcagcactg cgtgaacaat ttgcctggct gcgcgagaac ggttatcaac
     2761 cggtcagtat tgctcaaatt cgtgaagcac atcgaggagg aaaaccgcta ccggaaaaag
     2821 ctgtagtgct gacttttgat gacggctacc agagttttta tacccgcgtc ttcccaattc
     2881 ttcaggcctt ccagtggcct gctgtatggg cccccgtcgg cagttgggtc gatacgccag
     2941 cggataaaca agtaaaattt ggcgatgagt tggtcgatcg agaatatttt gccacgtggc
     3001 aacaagtgcg agaagttgcg cgttcccggc tcgttgagct cgcttctcat acatggaatt
     3061 ctcactacgg tattcaggct aatgccaccg gcagcttatt gcctgtatat gtaaatcgtg
     3121 catattttac tgaccacgca cggtatgaaa ccgcagcaga ataccgggaa agaattcgtc
     3181 tggatgctgt aaaaatgacg gaatacctgc gtacaaaggt tgaggtaaat ccacacgttt
     3241 ttatttggcc ttatggcgaa gcgaatggca tagcgataga ggaattaaaa aaactcggtt
     3301 atgacatgtt cttcaccctt gaatcaggtt tggcaaatgc gtcgcaattg gattccattc
     3361 cgcgggtatt aatcgccaat aatccctcat taaaagagtt tgcccagcaa attattaccg
     3421 tacaggaaaa atcaccacaa cggataatgc atatcgatct tgattacgtt tatgacgaaa
     3481 acctccagca aatggatcgc aatattgatg tgctaattca gcgggtgaaa gatatgcaaa
     3541 tatcaaccgt gtatttgcag gcatttgctg atcccgatgg tgatgggctg gtcaaagagg
     3601 tctggtttcc aaatcgtttg ctaccaatga aagcagatat ttttagtcgg gttgcctggc
     3661 aattacgtac ccgctcaggt gtaaacatct atgcgtggat gccggtatta agctgggatt
     3721 tagatcccac attaacgcga gtaaaatact taccaacagg ggagaaaaaa gcacaaattc
     3781 atcctgaaca atatcaccgt ctctctcctt tcgatgacag agtcagagca caagttggca
     3841 tgttatatga agatcttgcc ggacatgctg cttttgatgg catattgttc cacgatgatg
     3901 ctttgctttc agattatgaa gatgccagtg caccggctat cacggcttat cagcaagcag
     3961 gctttagcgg gagtctgagc gaaattcgac aaaacccgga gcaatttaaa cagtgggccc
     4021 gctttaaaag tcgtgcgtta actgacttca ctttagaact tagtgcgcgc gtaaaagcca
     4081 ttcgcggtcc acatattaaa actgcacgaa atatttttgc acttccggta atacaacctg
     4141 aaagtgaagc ctggtttgca cagaattatg ctgatttcct aaaaagctat gactggaccg
     4201 ctattatggc tatgccttat ctggaaggtg tcgcagaaaa atcggctgac caatggttaa
     4261 tacaattgac caatcaaatt aaaaacatcc ctcaggctaa agacaaatct attttagaat
     4321 tacaggcaca aaactggcag aaaaatggtc agcatcaggc tatttcttcg caacaactcg
     4381 ctcactggat gagcctatta caactgaatg gagtgaaaaa ctatggttat tatcccgaca
     4441 attttctgca taaccaacct gaaatagacc ttattcgtcc tgagttttca acagcctggt
     4501 atccgaaaaa tgattaatcg catcgtatcg ttttttatat tatgtctggt gttatgcata
     4561 cccctatgcg tagcgtactt tcactctggt gaactgatga tgaggttcgt tttcttctgg
     4621 ccgtttttta tgtccattat gtggattgtt ggcggcgtct atttctgggt ctatcgtgaa
     4681 cgccgctggc cgtggggaga aaacgcacca gctccccagt tgaaagataa tccgtctatc
     4741 tccattatca ttccctgttt taatgaggag aaaaacgttg aggaaaccat acacgccgct
     4801 ttagcacagc gttatgagaa cattgaagtt attgccgtaa atgacggttc aacagataaa
     4861 acccgtgcca tcctggatcg catggctgca caaattcccc atttgcgggt cattcatctg
     4921 gcgcaaaacc aggggaaagc cattgcgctt aaaaccggag ctgccgcggc gaaaagtgaa
     4981 tatctggtgt gcattgatgg cgatgcgtta ttagaccgcg atgcggcggc atatattgtg
     5041 gaaccgatgt tgtacaaccc gcgtgtgggt gccgtaaccg gtaatcctcg tattcgaaca
     5101 cgttctaccc tggtgggtaa aattcaggtt ggcgagtatt cctcaattat tggtttgatc
     5161 aagcgaaccc agcgtatcta tggaaacgta tttaccgttt ccggtgttat tgccgcattt
     5221 cgtcgcagcg ccctggcaga agtgggttac tggagtgacg atatgatcac cgaagatatt
     5281 gatattagct ggaagctgca gttgaatcag tggacgattt tttacgagcc acgggcactg
     5341 tgctggatat taatgcctga aacgttaaaa gggctgtgga aacagcgcct gcgctgggct
     5401 cagggcggtg cagaagtatt cctcaaaaat atgacaaggt tgtggcgcaa agaaaacttt
     5461 cgaatgtggc cgctgttttt tgaatacagc ctgacgacaa tatgggcctt cacctgcctg
     5521 gtcggtttca ttatttacgc agtccaactt gccggtgtac cgttaaatat tgaattgaca
     5581 catatcgctg cgacacatac tgccggaata ttattgtgta cgttatgttt actgcaattt
     5641 attgtcagcc tgatgatcga gaatcgctat gagcataatc tgacttcatc gcttttctgg
     5701 attatttggt tcccggttat tttctggatg ctgagcctgg caacgacatt ggtatcattt
     5761 acacgagtca tgttgatgcc taaaaagcaa cgcgcccgtt gggtaagtcc cgatcgcggg
     5821 attctgagag gttaatatga acaatttaat tattacgacc cgacaatcac cagtacgttt
     5881 actggttgat tatgttgcca caaccatctt gtggacatta tttgcgttgt tcatattctt
     5941 attcgccatg gatctgctga cgggttatta ctggcaaagc gaggccagaa gccgacttca
     6001 gttctatttt ttgctggcag tggcgaatgc cgtcgtgtta attgtctggg cgctgtacaa
     6061 taagctgcgt tttcaaaaac agcagcatca tgcagcctac caatatacgc cgcaagaata
     6121 tgcagagagc ttagcaatac ctgatgagct ctatcagcaa ctacaaaaaa gccacaggat
     6181 gagcgtacac ttcaccagcc aggggcaaat aaaaatggtt gtttcagaaa aagcgctagt
     6241 cagggcataa acacccaaaa caaagcccgg ttcgcccggg ctctgcaccg ataatacact
     6301 taactgtatg catgcagcgt acgttggcaa agtgccgaac gtacgcaatc ctcttttccg
     6361 aaccggacga tcccaaccat ttcatcttct tcgaaacgtt ccagcgcgtc acttaatccg
     6421 gagcacacgc cgcgaggcaa atcgcattgc gtgatatcac cgttgacgat aaccgtcacg
     6481 ttctccccga ggcgggttaa aaacattttc atttgcgcgg cagtcacatt ctgcgcctcg
     6541 tcaagaatga cgactgcatt ttcaaaggta cgtccacgca tataggcgaa cggcgcaatt
     6601 tccaccttcc ctatttccgg tcgcaggcag tactgcataa aggaagcccc taagcgccgg
     6661 accagcacgt cgtagaccgg gcgaaaatag ggagcaaact tttctgcgat atctccaggt
     6721 aagaagccaa gatcttcatc ggcttgcaga actggacggg tgacgataat cctgtcgaca
     6781 tccttatgta tcagggcctc tgccgctttt gctgcgctga tccaggtttt tccgcacccg
     6841 gcttcgcccg tggcgaatat cagctgctta ctctcaatag ccttcagata gtgcaattgc
     6901 gcttcatttc gcgcgaggat gggcgaagta tcgcgactgt cgcgggccat accaatggct
     6961 tctacgccgc ccatctgcac aagcgaggtg accgattctt cttcacgctg cttatggctg
     7021 cgcgaatccc gtctcagcac acgttttgcc tcgcgacgag ctttgatcac tgctttttgt
     7081 cttcccatgg agagcacctt gagttgtttg tattcatcac acgcgccgtt ggcagcgcga
     7141 ttatgcgcac gaacatcaga gggttggctt ccttgtaagc catagtttgc ttttggataa
     7201 aatgccgaaa aacggctacg cgcaccgttt acggcgtcgg taacacatga aaagaaagga
     7261 tgaggttgaa aatgcaaagt gacgagatga ctaccggagg agaaaactcc gcgagtggtg
     7321 gcgcgttgat tatctaaaac atgtccagta caggacgtta ccatccgcga tctccatagt
     7381 gactgactat cactgccggg aacttccgct gctacttaat aagtacaaca gttctcgcat
     7441 ttattgcaac aatatattta cttatattta actataaaac accatttcag tgacattagt
     7501 ttctactgga aagatgacag agtgatgaca gatatgaaaa aagctgtgtg ctttcagcag
     7561 gatttgcatc aggcatcagt gcacgatcgc cgtatgcggc gtgaacgcct tatccgccta
     7621 ccgaacccgc accgattgta ggcataagaa gacgcgcaag catcgcatca ggcatctgcg
     7681 caagaacgcc ttatccgtct aaaaacatta aacccgcaat aacgcgcttc cgagataatc
     7741 gttcgcgtcc ttcactcccg gcagcgcaaa aaaataaccg ccgccgatag gtttaacgta
     7801 ttcctccagc gcttcgccat tgagcctttt ttgtactgtc aggaagcctt tttccagatc
     7861 gtgttggtaa cagacaaaca gtagccccat atccagttgc ccggagttgg tgacgcccag
     7921 tgaataactg tagccacgac gcagcatcag gctggactca ctctccgccg tgcggggatt
     7981 cgccagccgg atatggctgt ccagcgcaat ccccttccct tccgggtcgc tggcgtaatc
     8041 aggcacatca tgctcatgct gcattcccag cggcgcaccg gtttgcttat cacggccaaa
     8101 aatcgtctgc tgttctttca gcggcgttct gtcccaaaat tccactcgaa actgaatcaa
     8161 gcgtactgcc tgatagctgc caccgattgt ccacgcaggc tcctgctgat ctgccgttac
     8221 ccacaccact ttttgcatca acttatcatt ctggctatcg ggattagcag tgccgtcttt
     8281 gaaacccagc aaattaatcg gcgtctcttt gcctttacta cgcgccgcgt gatcggaaat
     8341 aaacccttcc cgcttccagc gcacgctgag caaatccggc gtatgtttga tgatatcgcg
     8401 cagtgcatgg atcaccgtgt cctgggtgtt ggcgcaaatt tgtagcaaca catcaccatg
     8461 acataacgcc gcatccagcg agtcgttggg aaaacgcgtc atcttctgca actttttcgg
     8521 catctgtggc gcaaggccaa agcgctcatc aaacaatgag tgacccaccg ataacgtgat
     8581 ggtgagatta tcgggcgcaa tgtagccgcc aagaatgccg gaatcgagtg gtggcaggcg
     8641 cggatttggc gtttctggtg ccgctccgcc ctgagtcaga aaagcaaaac gctgagtcaa
     8701 caagcgaaac aaccgttcaa gatcggcttt atcgctggca agcacatcaa acgccaccag
     8761 catcattgcg gcctgttgtg gcgtcaggat ccctgcctga tgctcaccat aaaacggctg
     8821 tttctcattg cgtgcaaccg gtgaaagcgt acccggcgca ctttgcgttt tttgtgcatg
     8881 agcgaccgga caacttcccg ccagcgccag cgcaccaatg cctttcagta aacgtcggcg
     8941 tgacggttca ttcacgccgt ttttatcttc atactgcata atgcttaatc caatcccagc
     9001 acaccgcgaa gttgcgccag atcttccgcc agcgcagtaa tcggtccttt cagtgcattc
     9061 cggtcggcat cggtcaattt gtcgtaggtt tcaaaaccgt ctttagtacg gtatttcgcc
     9121 agaatggtat cgaccttttt aaagttggca tcgacttttg ccaacagttc cgggttagct
     9181 ttttgcagtt gtggacgcag cagatcgaca attttctgcg agccttcaac gttagcctgg
     9241 aaatcccaca gatcggtgtg gctgtagcga tcttcttcac cgctgatttt gctggctgcc
     9301 acttcctcaa tcagtccggc tgcaccgccg accacttttg aaggtgggaa agccagttca
     9361 ctgatgcgtt tttgcaaatc gaccacatcg gtataaagct ggtcagcgta ctgatccatg
     9421 cctttggtgg tgttgtcgcc aaacaatgct ttttccagac ggtggaaacc ggtgaatttt
     9481 ggatcagcgg ctttttgctc gtaatcatct tcacgggcgt caatgctgcc atccagatcg
     9541 gagaacagtt cagcaatcgg ttcaatgcgc tcatagtgct ggcgcgtcgg cgcatacagt
     9601 gctttcgctt tttcgatatc gcctgcttta atcgcgtcgg taaaggcttt ggtgtcggtc
     9661 accagctgtg tggtttccgc catgacatac gctttatatg cagtaattgc accaccaaga
     9721 cttaacagcg catcactttg cgccgcatcc gccgttgcct cacctttgac gatcaacttc
     9781 cctttcgggt tagtcagcag accgcaggtc atatcgtatt cgccaggctg taaattcgcc
     9841 gtcattttct ggctaaagcc aggggcgata ttttcccgct cttccaccac catcacgcct
     9901 ttgaggatct cccattccag cgccttctgg ctgtggttct gaataatgaa ctgtgttttc
     9961 ccggcgttaa ccgtaatggt catcggttcg cactgcttat ccgtcacggt cactttgacc
    10021 tgcggcacat cagcggcgtt agccataaaa gcagaagaaa acagcgcagc cacgctcaac
    10081 tgcaatgcgt tacggcggaa gttaatggtc atgacgagac tatcccttta aagtatgttg
    10141 taactaagca agagtttgcg tcgtatttgt tacgcggagc gagacgctgt cgcccctgcg
    10201 cgtggtggca gagcaaatgc caccagcgcc gggatgagat aaataaacca gacggcgact
    10261 tcgctgacgc tcggcgcttc ctgatagcca aaaatccctt ccatcagcgt gccaaacagc
    10321 gagtgagttg agagcaccgc actcatatcg aaggcgattt cctgaaagtg gttccacaat
    10381 ccggcttcat gaaatgcgcg aatggcacca gctgccagcc ctgcggcgac gaagagaata
    10441 aacaggctgg tccatttaaa aaatgcacca agattgaggc gaataccgcc ccagtagagc
    10501 aggaagccta gcaccacggc agtagcaaga ccgagcattg cacccagcgg cggccagatc
    10561 ccgacatctt gttgaaatgc cgccagcagg aaaaagaccg actccagccc ttcccttgca
    10621 acggcaaaaa agaccatcat caccagcgcc cagccatgat gatttccacg ctgcaatgcg
    10681 ctatcgacag cctgctccag ttgtactttg acgttgcgcg acactttgcg catccagaaa
    10741 accatccagg taaggatcac cacggcgatc accgccacga taccttcaaa cagttcctgc
    10801 tctttttgcg gaaattcgcc ggtggtttcg ttaatgaaga tccccaagcc caggcacaac
    10861 gcagcggcaa gcaacacgcc aatccacatc acaccaatcc atcggcctcg ctgggtacgc
    10921 ttaagatagc tggcaatcaa actgacaatc agcgcggctt caagtccttc gcgcaacata
    10981 atgagaaacg gaacaaacat gccacccacc cttaaacgtt tttctgagtc aatctatgca
    11041 aagaaaagta aatcgcagtg atagcgatta tcattacgct aaagaaaaat acaagcggaa
    11101 tatttataaa atgatgttgg aatgtaaaaa tgaggagatc aaatccggag aaaaaagcga
    11161 ttattttaag gtatttagct gacgtttcgc ccggcagatg aagcttgtac gaatgttggt
    11221 ggcgacaaac tcgctgcagc gtgcccgaag ggttgaattt tcagttagtc gtttcacaaa
    11281 agaacgttgt tttttgctta ttaatagata gtgataaggt tgtgaatgaa attatacctc
    11341 atctcctcat cgaatttttt gcccacaacc taaacaggag aaaacgtgta tttaagtctg
    11401 gaatgagcga tttttgatag tgcctaatta tccccca
//
LOCUS       DBFIFI010000134        11419 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.134, whole genome shotgun sequence.
ACCESSION   DBFIFI010000134 DBFIFI010000000
VERSION     DBFIFI010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.134"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(72..824)
                     /gene="tcyN"
                     /locus_tag="N7B39_003371"
     CDS             complement(72..824)
                     /gene="tcyN"
                     /locus_tag="N7B39_003371"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HGU2423292.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARKLLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPEQPRTRQFLEKFLLQ"
     gene            complement(821..1489)
                     /gene="tcyL"
                     /locus_tag="N7B39_003372"
     CDS             complement(821..1489)
                     /gene="tcyL"
                     /locus_tag="N7B39_003372"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HGU2423293.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     TAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(1504..2490)
                     /gene="dcyD"
                     /locus_tag="N7B39_003373"
     CDS             complement(1504..2490)
                     /gene="dcyD"
                     /locus_tag="N7B39_003373"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HGU2423294.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASVEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(2595..3395)
                     /gene="tcyJ"
                     /locus_tag="N7B39_003374"
     CDS             complement(2595..3395)
                     /gene="tcyJ"
                     /locus_tag="N7B39_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU2423295.1"
                     /translation="MKLAHLGRQALMGVMAVALIAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTADDLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(3483..4034)
                     /gene="fliZ"
                     /locus_tag="N7B39_003375"
     CDS             complement(3483..4034)
                     /gene="fliZ"
                     /locus_tag="N7B39_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HGU2423296.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDENGWQTEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(4080..4799)
                     /gene="fliA"
                     /locus_tag="N7B39_003376"
     CDS             complement(4080..4799)
                     /gene="fliA"
                     /locus_tag="N7B39_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HGU2423297.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(5119..6876)
                     /locus_tag="N7B39_003377"
     CDS             complement(5119..6876)
                     /locus_tag="N7B39_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliC/FljB family flagellin"
                     /protein_id="HGU2423298.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQATTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGETITIDL
                     KKIDSDTLGLNGFNVNGKGTITNKAATVSDLTSAGAKLNTTTGLYDLKTENTLLTTDA
                     AFDKLGNGDKVTVGGVDYTYNAKSGDFTTTKSTAGTGVDAAAQAADSASKRDALAATL
                     HADVGKSVNGSYTTKDGTVSFETDSAGNITIGGSQAYVDDAGNLTTNNAGSAAKADMK
                     ALLKAASEGSDGASLTFNGTEYTIAKATPATTTPVAPLIPGGITYQATVSKDVVLSET
                     KAAAATSSITFNSGVLSKTIGFTAGESSDAAKSYVDDKGGITNVADYTVSYSVNKDNG
                     SVTVAGYASATDTNKDYAPAIGTAVNVNSAGKITTETTSAGSATTNPLAALDDAISSI
                     DKFRSSLGAIQNRLDSAVTNLNNTTTNLSEAQSRIQDADYATEVSNMSKAQIIQQAGN
                     SVLAKANQVPQQVLSLLQG"
     gene            7142..8539
                     /gene="fliD"
                     /locus_tag="N7B39_003378"
     CDS             7142..8539
                     /gene="fliD"
                     /locus_tag="N7B39_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HGU2423299.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSSTTAFSATTAGNAIAGKYTISVTHLAQA
                     QTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNAKAG
                     VSASIINVGNGDYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEVSVA
                     AQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIKDWV
                     NAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVSSSS
                     YKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGSNLT
                     SWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSLNST
                     SSYLTQQFENNSNSK"
     gene            8564..8974
                     /gene="fliS"
                     /locus_tag="N7B39_003379"
     CDS             8564..8974
                     /gene="fliS"
                     /locus_tag="N7B39_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HGU2423300.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVETLMRNIADAWKESLLSPSLIQDPV"
     gene            8974..9339
                     /gene="fliT"
                     /locus_tag="N7B39_003380"
     CDS             8974..9339
                     /gene="fliT"
                     /locus_tag="N7B39_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HGU2423301.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVDPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            9417..10904
                     /gene="amyA"
                     /locus_tag="N7B39_003381"
     CDS             9417..10904
                     /gene="amyA"
                     /locus_tag="N7B39_003381"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HGU2423302.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKVQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPNEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRNYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVL"
     gene            complement(10938..11351)
                     /gene="yedD"
                     /locus_tag="N7B39_003382"
     CDS             complement(10938..11351)
                     /gene="yedD"
                     /locus_tag="N7B39_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HGU2423303.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
BASE COUNT         2832 a         2960 c         2815 g         2812 t
ORIGIN      
        1 taagcagttt tatcaggctt gaaatggcgt tccgccccga ctgtcgaatc gtcggggctg
       61 attttttctt attattgcag cagaaacttc tcgaggaact ggcgggtgcg aggctgctcg
      121 ggatcggcaa ataacgcttt tgcggccccc tgctcgacta tccgcccctg gtccataaaa
      181 atcgcccggt ccgcaacatc ccgggcaaag ctcatttcgt gtgtcacaat caccatcgtg
      241 cgcttttcct gcgccagctg acggatggtg ttcaggactt cgcccaccag ctctggatcc
      301 agcgctgacg ttggctcgtc aaacaaaatc acctcaggac gcattgccag cgcacgcgca
      361 atcgcaacac gctgctgttg accgccagac aaacgacgtg gatagctggt ttctttaccg
      421 gccagcccaa ctttcgccaa cagcttgcga gcgcgcgccg tggcctcttc tttcggttcg
      481 cctttgacga tcaccggccc ttcaataatg ttctccagca ccgtacgatg cggaaacaaa
      541 ttaaagttct ggaagacaaa cccgacgtgc tgacgcaact ggcgaatcag cgatttttgc
      601 tgacttaatg aacgtgcagt atcaatagtg atatcgccaa cggtgatcgt tcccgcttcc
      661 ggttgttcca gcagatttat gctacgtagc aatgtggttt tgccggaacc actcggaccg
      721 ataattgcca ccacttcgcc aggctttacc tcgaggtcga taccgtgcag caccgtctga
      781 ccgtggaatt ttttcactag gttcttaact tcaatggcac tcatttgggt tctctctcct
      841 ggcgattaag ttgattctca aaatggttct gcaacgtcga taacacagtc gccatgatcc
      901 agtagatgag cgaagccgcc agatacatgg tgaatacctc cagcgtacgc gaggtaatca
      961 actgcgcctg acggaacagc tccggcacct ggattgtcgc ggccagcgac gtatctttta
     1021 ccagactgat aaagctgttc gagagcggcg gcaacgccac ccgcgccgcc tgtggcaaaa
     1081 tcgcgcgacg catagtctgc catggcgtca taccaatact ggcagctgct tcccactgac
     1141 ctttatcaat agaagaaatg gcggcgcgaa gcgtttcggc agtataggcg gcagtgttga
     1201 gcgacagacc aatcattgct gacggaatgg gatctaattc gataccaaac tgcggcaggc
     1261 cgtaatagat cataaacagt tgtgcgatga gcggcgtacc acgaaaaata gagatataaa
     1321 aacgcgccag ccagcgcacg ggccagattg gcgacaggcg catcagcgcg agaataaacc
     1381 ctagcagtaa accaaaaaac atgccgccga tactgagttg cagcgtatac ccagcccctt
     1441 tcagcaggaa cggcaaggaa tcaataacca gttgtatact ttcttgcatg agcgttcccg
     1501 gagctaaacg tggggatgat aggcgaacag cgcaggcgcg ccgccggtat gaataaacag
     1561 aatcggccct tcatctttga agcgtttctg actgataccg tcaatcagac ccgccatcgc
     1621 ttttccggta tacacaggat caagcagaat gccttccagc cgcgccagca atttcactgc
     1681 ttccatgcct tcgtcgttcg gcacgccgta gccaggtgca aaatagtcat cccagagtaa
     1741 aatttccact gatgcggtca gctccagttc tttcgcaatc gcctgttgta ggttaaccac
     1801 tttcggcaat tgatcggcaa cggaacgcga cacggtcacg ccaatcagtt cgctttcagg
     1861 catcaggtgt tccagcccaa cagccagtcc ggcgtgagtt ccggcactgc ccgatgcgac
     1921 taccaccgat gaaatattaa ccgccccttc acactgttgc gcgatttcca gcgcactctc
     1981 cacataacct agcgcgccaa gagcattaga accgccaacc ggaatgacat acgggcgaaa
     2041 gccttgtgct tcgactcgcg tcgccagctc ttccagttgg gcattgggat cggtcagtgc
     2101 gtcgcacatt tcaatctggg tattgaacag atccagcaac aagcgattgc cgttggttaa
     2161 atagttttct gcggttgtgc caataggatt ttccagcagc gccacgcagt gcagaccgag
     2221 tttggcagcg actgcggcag tctgccgcac atggttagac tggatcgccc cggcagtaat
     2281 cagcgtatcg gcaccttcac gcagagcatc tgccgcgaga aattctagct tacgtaattt
     2341 attgccgccc attgccatgg gggtgacgtc atcccgtttg atgaaaattt cccgtcctag
     2401 ataatcagaa aagcgcggca gatattcgag cggcgttggc gcgccgataa actccagccg
     2461 tggaaaacgg gttaaattat gcagtggcat aacagcctcc gatgtgtgtt gttgtgattt
     2521 tctcattatg cacgctgaaa acgcgtaaat aaaaaaggcg ctagtgaaag cgcccttttt
     2581 tgtcattatg ctgattattt ggtcacatca gcaccaaacc atttttcgga aagggcttgc
     2641 aaagtgccgt ctttttgcat ttccgcaatt gcatcattca ctgctttcag caggtcttca
     2701 tttcctttac gcagcgccac gccagactcc tgacgggaga atgcttcacc ggttactgcc
     2761 agcgtatcgt tggttttctt caccagatcc agcgccgcca gacgatcaac gaggatcgca
     2821 tcgatacgcc ctacgcgcag atcctgatat ttggtcgggt catcatcata ggtacgcaca
     2881 tcgacgccct gaacattctg ccgcagccac tcttcatagt tggtgcccag accgaccccc
     2941 acttttttgc ctttcagatc atcggctgtt ttaatggtgc cttcgttacc ttttttcacc
     3001 agcgcctgaa taccagaaat ggtgtacggg gttgagaaat cgtatttttt cttgcgctca
     3061 tcagaaatgg tgacctgatt aatcaccaca tcaatacgtt tagagtccag cgacgccagc
     3121 ataccgtccc atttggtcgg ttttagtgac gcctcaacgc caagatgttt tgccagctgt
     3181 tgggcaaatt ccacttcaaa accggttaat ttgccgtcat ccccctgaaa actgaacggc
     3241 ggataagtac cttccagccc taccagcagc gtgccgcgct ctttaacttt attaagcaga
     3301 ccttcatctg caaaactttt aacgctcatg cccgcaatca gcgctacggc catcacaccc
     3361 atcaatgcct gacgtcccag atgtgctaat ttcatattca ccccgaatgt tgttatgtct
     3421 gtttgcagtg tagagccatt gtttgtaaac acaaaaacaa ctccgctaca tcttattctt
     3481 atttaatata tatcagaagc aggcaggctg gaggatttct gcacaagtcc ggtacaggtt
     3541 tggcgctgat agtgttgata cttccgtaac gcgatgcggt aattgttggt gctggtggtg
     3601 ggcagccacg gggcgaggat ttcatcaagg aaaccgtcct gcagctgatc gagggaaata
     3661 ttttgctcgt gtagatgatt ccccaaacgg cgcagacgaa cgacatattc acgcaccgta
     3721 ccagggctca tttcagtttg ctcaaaaaga aattgcttaa agccgataat atcgaaaaaa
     3781 tcactctgcg ttttgcaatg taaatcaccg caaaatcggc acaatgccgc ccatgatttt
     3841 tgttccgttt gccagccatt ttcatcaagc agcgtgtcca ggcgggaaat ctcgatttta
     3901 ttcactattt tgccgtcgcg aactaaggta atgcgatcga gcaatttacg gcaatgcgcg
     3961 caatgggtct ggctgtgttt aaagtcttta agatagcggc ttaatggccg tcttttcagg
     4021 tgctgcacca tcattaagaa ctcctggtag tcaaagttaa agtgcggcat ttactgacgt
     4081 tataacttac ccagtttagt gcgtaaccgt ttaatagcct ggctgtgtaa ctgactgacc
     4141 cgcgattccc cgacctccag cactgcacca atctctttga gattcagctc ttcctggtaa
     4201 tagagggtta ataccagttt ttcgcgctcc ggcaacgttt cgatagcttc catcacccgc
     4261 tggcgcagat tactgtccag tagttgttgt agcgggtttt ctcgttgatg atcatcagta
     4321 accaattcga tgctatcgcc gtgctcttcg cgccactcat cgtaggagaa gagctggctg
     4381 ttattggtgt cgagcaacat ttggcgataa tcggcaatat cgatccctaa acgttccgct
     4441 acctcagttt ccgtggcgtt gcggccaagt tcctgctcca gttgccctat tgcctgtgcc
     4501 acttcacgcg cgttgcgtcg cacgctgcgc ggcacccagt cacggctgcg aagttcatcc
     4561 agcatagcgc cacggatacg ctgcactgcg taagttgtaa atgccgttcc ttgtagggcg
     4621 tcatagcgtt cgacggcatt aagtaaccca atgccgcccg cctgtagcag atcgtcaagt
     4681 tccacgctcg cgggcagtcg aacctgcagg cgcaatgctt cgtgacgcac cagcgggaca
     4741 taacgctgcc acagcgagtg tttatccatt acaccttcag cggtatagag tgaattcacg
     4801 ataaacagcc ctgcgttata tgagttatcg gcatgattat ccgtttctgc agggttttta
     4861 atcggacgat tagtgggtga aatgaggggt tatttggggg ttacaggtaa attccaggca
     4921 gaaaaaaccc cgccggtggc ggggaagcac gttgctgaca aattgcgctt tatgttgccg
     4981 gatgcggcgt aaacgcctta tccggcctac aagaatgtgc aaattcaata aattgcaatt
     5041 caacttgtag gcctgataag cgcagcgcat caggcaattt ggcgttgccg tcagtctcag
     5101 ttaatcaggt tacaacgatt aaccctgcag cagagacaga acctgctgcg gtacctggtt
     5161 agcttttgcc agcacggagt taccggcctg ctgaatgatc tgcgctttcg acatgttgga
     5221 cacttcggtc gcatagtcgg cgtcctgaat acgggactgc gcttcggaca ggttggtagt
     5281 ggtgttgttc aggttggtga ctgcggaatc cagacggttc tggatagcac ccagggaaga
     5341 acggaatttg tcgatggagc tgattgcgtc gtccagggca gcaagcgggt tggtcgttgc
     5401 agaaccagca ctggtagtct cagtagtgat tttacccgcg gagttcacat ttacagcagt
     5461 accaattgct ggagcataat ctttattggt atcagtcgct gaagcatacc cggcaacagt
     5521 cacagagccg ttatccttgt taacgctgta agagactgta tagtcggcaa cgttagtgat
     5581 accaccttta tcatccacat aagacttcgc agcatcactg gattcacccg cggtaaaccc
     5641 aatagttttg ctcagtacac cggaattaaa ggtaattgaa gatgtcgcgg cagccgcttt
     5701 ggtttcgctc aatactacat ctttactcac tgtagcctga taagtaatcc caccagggat
     5761 taacggagct actggagtgg ttgtcgcagg agttgctttt gcgatggtat attctgtgcc
     5821 attgaatgtc agagaggcac cgtcactacc ttcgctcgct gctttgagca gcgctttcat
     5881 atcagcttta gctgcgctac cagcgttgtt agtcgtcaag ttgcctgcat cgtctacgta
     5941 tgcctggctt ccaccgatgg tgatattacc tgctgaatcc gtttcgaaag aaacagtacc
     6001 atcttttgtg gtgtaagaac cattaacaga tttacccaca tcagcatgaa gggtggcagc
     6061 taacgcatca cgttttgaag ctgaatcagc agcctgcgcc gcggcgtcta cacccgtacc
     6121 agcagtagat ttagtggtag taaaatcacc agatttagcg ttgtaagtat aatctacgcc
     6181 gccaactgtg actttatcgc cattccctaa tttatcgaat gcagcatcgg tagttaacaa
     6241 ggtattttcg gttttcagat cataaagacc tgtcgtggtg tttaacttcg cgccagcaga
     6301 agttaaatca cttaccgttg cagctttgtt ggtaatagta cctttaccat ttacgttaaa
     6361 gccattcaga cccagagtat cagaatcgat ttttttcagg tcgatcgtga tggtttcgcc
     6421 gtcattcgca ccaacctgaa ttttcattga accgtctttc gccagcacgt tcacgccgtt
     6481 gaactgggtc tggccggata cgcggtcaat ttcatcaaga cgagatttga tttcgtcctg
     6541 gatggagtcc aggtcagaat cggagttagt ccctgtagtg gcctgaaccg tcagttcacg
     6601 aatacgctgt aagttgttgt tgatttcgga cagcgcgcct tcggtggtct gcgcaacaga
     6661 aataccgtcg ttggcgttac gggccgcctg agtcaggcct ttaatgttag aagtaaaacg
     6721 gttagcaatc gcctgacctg cggcgtcatc cttcgcgctg ttaatacgca agccagaaga
     6781 cagacgctcg atagaactcg acagcgcaga ctggttcttg ttgatattat tttgagtgat
     6841 cagcgagagg ctgttggtat taatgacttg tgccatgatt cgttatccta tattgcaagt
     6901 cgttgattac gtattgggtt tccacccgtc ggctcaatcg ccgtcaaacc tgttatcgtc
     6961 tgtcgtaaaa caacctttag aatttttttc acaaacagcc attttttgtt agtcgccgaa
     7021 atactctttt ctctgcccct tattcccgct attaaaaaaa acaattaaac gtaaactttg
     7081 cgcaattcag gccgataacc ccggtattcg ttttacgtgt cgaatgataa aaggaaatcg
     7141 catggcaagt atttcatcgc tgggagtcgg gtcaggtctg gatttaagtt caatccttga
     7201 tagcctcacc gccgcgcaaa aagcgacgct aacccccatt tcaaatcagc aatcgtcgtt
     7261 taccgctaaa cttagcgcct acggtacgct gaaaagcgcg ctgacgactt tccagaccgc
     7321 caatactgca ttgtctaaag ccgatctttt ttccgccacc agcagcacca ccgcgttcag
     7381 tgccaccacc gcgggtaacg ccatcgccgg gaaatacacc atcagcgtca cccatctggc
     7441 gcaggcgcaa accctgacca cgcgcaccac cagagacgat acgaaaacgg cgatcgccac
     7501 cagcgacagt aaactcacca ttcaacaagg cggcgacaaa gatccgatta ccattgatat
     7561 cagcgcggct aactcgtcgt taagcgggat ccgtgatgcc atcaacaacg caaaagcagg
     7621 tgttagcgcg agcattatta acgtgggtaa cggtgattat cgtctgtcag tcacatcaaa
     7681 tgacaccggc cttgacaatg cgatgacgct ctcggttagc ggtgatgatg cgctacaaag
     7741 ttttatgggc tatgacgcca gtgccagcag caacggcatg gaggtctcgg tcgccgccca
     7801 gaatgcgcag ctgacggtca acaacgtcgc cattgagaac agcagcaaca ccatcagcga
     7861 cgcgctggaa aacatcaccc tgaacctgaa cgatgtcacc acgggcaacc agacgctaac
     7921 catcactcag gacacctcca aagcgcaaac ggcgattaaa gattgggtga atgcctataa
     7981 ctcgctaata gataccttca gcagcctgac caaatacacc gccgtagatg cgggagctga
     8041 tagccagagt tctagcaatg gcgcactgct cggcgactcc acgctgcgga cgattcagac
     8101 gcagttgaag tcgatgctga gtaataccgt cagttcttcc agctataaaa cgttggcgca
     8161 gattggtatc acgaccgatc ccagcgatgg caaactggaa ctggatgccg acaaactcac
     8221 cgctgcactg aaaaaagatg ccagcggcgt aggtgcattg attgttggcg atggtaaaaa
     8281 aaccggcatc acgaccacca tcggcagcaa cctgaccagt tggctttcga caacgggcat
     8341 tattaaagcc gctaccgatg gcgttagtaa gaccctgaat aaattaacta aagactacaa
     8401 cgccgccagc gatcgcattg acgcgcaggt cgcgcgctac aaagaacaat ttacccaact
     8461 ggacgtttta atgacctcgt taaacagcac aagcagctac ttaacgcagc agttcgaaaa
     8521 caacagtaat tccaagtaat cattattcat cgggagacag gtcatgtacg cggcaaaagg
     8581 cacccaagcc tatgcacaaa ttggcgtcga aagcgccgta atgagcgcca gccagcagca
     8641 gctggtcacc atgctatttg atggagtgct gagcgcactg gttagagcgc gcctgtttat
     8701 gcaggacaac aatcagcaag gcaaaggcgt ctctttgtca aaagcgatca acatcattga
     8761 gaacggactg cgggtgagtc ttgatgaaga gagcaaagac gaactaaccc aaaacttgat
     8821 tgctctttat agctatatgg tcaggcgttt gctgcaagcc aatttacgca acgatgtctc
     8881 cgcagtcgaa gaagtggaaa cattaatgcg caatattgcc gatgcctgga aagagtcgtt
     8941 actctcccct tctttgattc aggacccagt ctgatgaacc atgcaccgca tttatatttc
     9001 gcctggcaac aactcgtcga aaaaagccag ctcatgttac gcctggcaac ggaagaacaa
     9061 tgggacgaac tcatcgccag cgaaatggcg tatgttaatg cggtgcagga gattgcacat
     9121 ttgactgaag aggttgaccc gtccaccacg atgcaggagc agctccgccc gatgctgcgc
     9181 ctgattctcg acaacgaaag caaggtaaag cagttattac agattcggat ggatgaactg
     9241 gcgaaactgg tcggtcagtc atcggtgcaa aaatcggtgt tgagtgccta tggcgatcag
     9301 ggcggctttg tgctggctcc gcaggataac ctcttttgaa tctgaatgag tcgatggctc
     9361 gcgaataatc cgattacggc tacgcttcta atgttcccct tgaatggagt cgaagaatgc
     9421 gtaatcccac gctgttacaa tgttttcact ggtattaccc ggaaggcggt aagctctggc
     9481 ctgaattggc cgagcgcgcc gacggtttta atgatattgg tatcaatatg gtctggttgc
     9541 cgcccgccta taaaggcgca tcgggcgggt attcggtcgg ctacgactcc tatgatttat
     9601 ttgatttagg tgagtttgat cagaaaggca gcatccctac taaatatggc gataaagtac
     9661 aactgctggc cgccattgac gccctgaaac gtaatgacat tgcggtgctg ttggacgtgg
     9721 tagtcaacca caaaatgggc gcggatgaaa aagaagctat tcgcgtgcag cgcgttaatg
     9781 ctgatgaccg tacgcaaatt gatgaagaaa tcattgaatg cgaaggctgg acgcgttaca
     9841 ccttccccgc tcgtgccggg caatactcac agtttatctg ggatttcaaa tgttttagcg
     9901 gtatcgacca tatcgaaaac cccaatgaag atggcatttt taaaattgtt aacgactaca
     9961 ccggcgaagg ctggaacgat caggttgatg atgaactcgg taatttcgat tatctgatgg
    10021 gcgagaatat cgattttcgc aatcatgccg tgacggaaga gattaaatac tgggcgcgct
    10081 gggtgatgga acaaacgcaa tgcgacggtt ttcgtcttga tgcggtcaaa catattccag
    10141 cctggtttta taaagagtgg atcgaacacg tacaggaagt tgcgccaaag ccgctgttta
    10201 ttgtggcgga gtactggtcg catgaagttg ataagctgca aacgtatatt gatcaggtgg
    10261 aaggcaaaac catgctgttt gatgcgccgc tgcagatgaa attccatgaa gcatcgcgca
    10321 tgggacgcaa ctacgacatg acgcagattt tcactgggac gctggtggaa gccgatcctt
    10381 ttcacgccgt aaccctcgtt gccaaccacg acacccaacc gttgcaagcc ctcgaagcac
    10441 cggtcgaacc gtggtttaaa ccgctggcgt atgctctaat tttgttgcgg gaaaatggcg
    10501 ttccttcggt gttctatccg gacctctacg gtgcgcatta cgaagatgtc ggtggtgacg
    10561 ggcaaaccta tccgatagat atgccaataa tcgaacagct tgatgagtta attctcgccc
    10621 gtcagcgttt cgcccacggt gtacagacgt tatttttcga ccatccgaac tgcattgcct
    10681 ttagccgcag tggcaccgac gaatatcccg gctgcgtggt ggtcatgtcg aacggagatg
    10741 atggcgaaaa aaccattcat ttaggagaga attacggcaa taaaacctgg cgtgattttc
    10801 ttgggaaccg gcaagagagt gtagtaaccg acgaaaacgg cgaagcgacc ttcttttgca
    10861 acggcggcag cgttagcgtg tgggttatcg aagaggtgct ttaaattcat ccccggcggc
    10921 aagccgggga gatttcatta cggcaatggc gtcggtaacg gcgctttatc cagcgcagcg
    10981 gcacactctg ccgtcgggcg atccacacgc tccatcgtca taccatcgta ctcaatggtg
    11041 ttaccgtcac gctcaacctc gtacagctca cgttttacgg taacgttagt gagatcatca
    11101 gacatcaatg tcaacttgcc cggcacagca atcacccgct gccactgacg gcaatctagg
    11161 gtatcgccct ctttagtgac aatcaggctg cctatcgctt ccgggctgac cagcgcacgt
    11221 tgcggccctt tggtctgcca gtagcccgcc agccagtccg gcgcgggggt tttcacgaca
    11281 ttgttatagt tttcgacttc ggcacagccc gccagcaaca tgagcgcgcc tgcaattgcg
    11341 agttttttca tcattcgtcc tgcatgagaa gaaaagagga ttgtggcatt aaagccctga
    11401 tgtcgccagc ctttccagg
//
LOCUS       DBFIFI010000135        11374 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.135, whole genome shotgun sequence.
ACCESSION   DBFIFI010000135 DBFIFI010000000
VERSION     DBFIFI010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.135"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..229
                     /locus_tag="N7B39_003383"
     CDS             <1..229
                     /locus_tag="N7B39_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HGU2423304.1"
                     /translation="YKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFLGSIPWERKT
                     VTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(231..752)
                     /gene="ruvC"
                     /locus_tag="N7B39_003384"
     CDS             complement(231..752)
                     /gene="ruvC"
                     /locus_tag="N7B39_003384"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HGU2423305.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(787..1527)
                     /gene="yebC"
                     /locus_tag="N7B39_003385"
     CDS             complement(787..1527)
                     /gene="yebC"
                     /locus_tag="N7B39_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGU2423306.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(1556..2008)
                     /gene="nudB"
                     /locus_tag="N7B39_003386"
     CDS             complement(1556..2008)
                     /gene="nudB"
                     /locus_tag="N7B39_003386"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HGU2423307.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(2126..3898)
                     /gene="aspS"
                     /locus_tag="N7B39_003387"
     CDS             complement(2126..3898)
                     /gene="aspS"
                     /locus_tag="N7B39_003387"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HGU2423308.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLRSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPAALAELSIQVVKKAENN"
     gene            4208..4774
                     /gene="yecD"
                     /locus_tag="N7B39_003388"
     CDS             4208..4774
                     /gene="yecD"
                     /locus_tag="N7B39_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HGU2423309.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILNAL"
     gene            4771..5589
                     /gene="yecE"
                     /locus_tag="N7B39_003389"
     CDS             4771..5589
                     /gene="yecE"
                     /locus_tag="N7B39_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HGU2423310.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNQVIL
                     DSRPVHAARPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            5642..6037
                     /gene="yecN"
                     /locus_tag="N7B39_003390"
     CDS             5642..6037
                     /gene="yecN"
                     /locus_tag="N7B39_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HGU2423311.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            6078..6821
                     /gene="cmoA"
                     /locus_tag="N7B39_003391"
     CDS             6078..6821
                     /gene="cmoA"
                     /locus_tag="N7B39_003391"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HGU2423312.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            6818..7789
                     /gene="cmoB"
                     /locus_tag="N7B39_003392"
     CDS             6818..7789
                     /gene="cmoB"
                     /locus_tag="N7B39_003392"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HGU2423313.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVS
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(7954..10383)
                     /gene="torZ"
                     /locus_tag="N7B39_003393"
     CDS             complement(7954..10383)
                     /gene="torZ"
                     /locus_tag="N7B39_003393"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HGU2423314.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGYNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFAELAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAQLRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDQPAVQA"
     gene            complement(10408..>11374)
                     /locus_tag="N7B39_003394"
     CDS             complement(10408..>11374)
                     /locus_tag="N7B39_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HGU2423315.1"
                     /translation="SKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASK
                     DIYHEFVSGKIDSDDKFEAHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESA
                     QKMHNKAQKGGETCIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTS
                     RIGELATVNPGTDLTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGI
                     KALTVNGEWQADEYGNQWRQASLQGALTDPALADRKLLWQYAEKLDDTYCAGCHAPIA
                     ADHYTVNAWPSIAKGMGARTSMSENELDILTRYFQYNAKDITEKQ"
BASE COUNT         2543 a         3040 c         2821 g         2970 t
ORIGIN      
        1 ttataaagaa gcgctatgta ttccggtggg tgaaatagag acgtttggag aattgttaaa
       61 tagcaatccg aatgcaaaac tcactttctg gaaattctgg ttcctgggtt ctattccgtg
      121 ggagcgtaaa accgtcacgc cagccagttt gtggcatcat ccaggtttgg tgttgattca
      181 tgcggaggga gtggaaacgc ctcagccgga actgaccgag gcggtataac ttaacgcagt
      241 cgccctctcg ccaggttcag ccgcgattcg ctcatctgca tcgcattctg actaacgtgg
      301 cagtgggtga tggcaatcgc cagcgcatcg gcggcatccg cctgtggatt agcgggcagt
      361 ttcagcaagg tgcggaccat atgctgcacc tggctttttt cggcactacc aatacctacc
      421 accgtttgct ttacctgacg tgccgcgtat tcaaatactg gcaactcctg attcaccgcc
      481 gccacaatcg ccacgccgcg cgcctggccc agtttcaggg ctgagtcagc gttctttgcc
      541 ataaagactt gttcaatggc gaaataatcg ggctggaact gggtgatgat ttccgtcacg
      601 cccgcataga tgagtttcag acgagacggt aaatcatcca ctttggtgcg tatgcatccg
      661 ctacccaggt aggacagttg cctacctacc tggcggatga cgccgtagcc ggtcacgcgc
      721 gaacccggat caatgccgag aataatagcc atcacgcgtc tccgttttgc tgtttagcag
      781 gcctcatcag agagtcgctg cgacctcatc agagatttca ccgttatggt aaacttcctg
      841 cacgtcgtcg caatcttcca gcatatcgat cagacgcatc agtttcggtg cggtttctgc
      901 atccatatca gctttggtag acgggatcat ggaaacttcc gcgctgtctg ctttcagacc
      961 tgccgcttcc agagcgtcgc gcactttacc catttcttcc catgcagtgt agacatcaat
     1021 cgcgccgtca tcataggtca caacgtcttc agcacctgct tccagtgctg cttccatgat
     1081 ggtgtcttca tcgcctttct cgaaggagat gacgcctttt ttgctgaaca gataggctac
     1141 ggaaccatca gtaccgaggt taccgccaca tttgctgaat gcatgacgca cttcagcaac
     1201 ggtacggttg cggttgtcag acagacattc aatcatgatt gccgtgccgc caggaccgta
     1261 accttcgtag atgatggttt ccatgttcgc atcatcatca ccgcccacac cacgtgcaat
     1321 tgcgcggttc agagtgtcac gggtcatgtt gttagacagt gctttatcaa ttgctgcacg
     1381 cagacgcggg ttagcgtccg gatcgccacc gcccagctta gccgcggtta ccagctcacg
     1441 aatgatttta gtgaagattt taccgcgctt agcatcctgc gcagctttac gatgtctggt
     1501 gttggcccat ttactatgac ctgccataaa aatatctcca gatagccctg cctgttcagg
     1561 cagcgttaat tacaaactgt tcaatcgcct gccggttgct ccaggactta gtgagcgccg
     1621 ccgcagcagg cgcatcaagc cacttgtaag ccagatgttc agtgaaaacg atctgccgct
     1681 catgcggaag cgcaagacag aaccatgatt ccgtattacg cgtcacgccc ggcgcatagc
     1741 gatgacgtaa atgtgaaaaa atttcaaact ctaccgtgcg ctgacagtca attaaggtca
     1801 gttgttcagc gacaacatca atggtgacct cttcctttac ttcgcgcatg gcagcttgcg
     1861 gcgcggtttc accctcttcc acgctgccgg ttaccgactg ccagaaatcg gggtcatcac
     1921 gccgctgcaa catcagcacc cgtttcgtat cttgtgcgta gatgaccact aagatcgaaa
     1981 cgggacgctt atacacttta tccttcacgc tgcctcttcg ttgactgcct tcgctcatcc
     2041 cattcacata gttatctatg ctcatgggag ttcactcagt tgccgcctcg atgcaacgcg
     2101 aatgatttcg tgtatttgag tcatatcagt tattctcagc cttcttcaca acctgaatgc
     2161 tcagctcagc cagtgcagcc gggttagcaa agctcggtgc ttcagtcatc agacacgctg
     2221 ccgccgtggt tttcgggaag gcgataacgt cacggatatt gtcggtgccg gtcagcagca
     2281 tggtcagacg gtcaagaccg aatgccagac ctgcgtgcgg cggagtaccg tatttcagag
     2341 cgtcgagcag gaagccgaat ttctcgcgct gttcctcttc gttgataccc agaataccaa
     2401 acaccgtctg ctgcatatca ccattatgga tacgtactga accaccgccc acttcgtaac
     2461 cattgatgac catatcgtaa gcgttcgcca ccgcattttc cggtgcagct ttcagctctg
     2521 cagccgtcat gtctttcggt gaggtgaacg gatggtgcat tgccgtcagg ccgccttcac
     2581 cgtcgtcttc aaacatcggg aagtcgataa cccacagcgg tgcccatttg ctttcgtcgg
     2641 tcagaccaag gtctttaccc actttcaggc gcagtgcgcc catcgcgtcg gcaacaattt
     2701 tcttgttgtc ggcaccgaag aaaatcatat cgccatcttg cgcggcagta cgatccagga
     2761 tggcttcgat gatttctgca ttaaggaact tcgctaccgg gctgttgata ccttccagac
     2821 ctttcgcgcg ttcgttaact ttgatgtaag ccagaccttt cgcgccgtag attttaacga
     2881 agttaccgta ttcgtcgatc tgcttacggg tcagcgatgc gccacccgga acacgcagag
     2941 cggcaacgcg acctttcgga tcgttcgccg gacctgcaaa tactgcaaac tcaacagatc
     3001 tcagcagatc agcaacgtcg gtcagttcca tcgggttacg cagatccggt ttatcagaac
     3061 cataacggcg ttcggcttcc gcaaaggtca ttactgggaa atcgcccaga tccacgccct
     3121 tcacttccag ccacagatgg cgcaccagcg cttccatcac ttcacgcact tgcggcgcgg
     3181 tcatgaaaga agtttccaca tcgatctgag taaattcagg ctgacgatca gcgcgcaggt
     3241 cttcgtcacg gaagcattta acgatctgat agtagcggtc aaagccggac atcatcagca
     3301 gctgtttgaa caactgtggg gattgcggca gtgcgtagaa tttacctttg tgcacacgag
     3361 aaggcaccag gtagtcacgc gcgccttccg gcgtggcttt ggtcagcatc ggagtttcga
     3421 tgtcgaggaa accgtggtca tccataaaac ggcgcaccag gctggtgatt ttagcgcggg
     3481 ttttcaggcg ctgagccatt tccggacgac gcaggtcgag gtagcggtat ttcagacgcg
     3541 cttcttcggt gttgacgtgg ttagagtcaa gcggcagaac atctgcgcgg ttgatgatag
     3601 tcagcgagga cgccagcact tcgatttcgc cggtcgccat atcgcggtta atattttttt
     3661 cgtcacgcgc acgtacggtg cccgtgacct gaatgcagaa ctcattacgc agttcagagg
     3721 ccagctttaa cgcgtccgca cgatccggat cgaaaaatac ctgcacgata ccttcgcggt
     3781 cgcgcatatc gatgaagatc aggctaccaa gatcacgacg acggttgacc caaccacaca
     3841 gagtcacctg ctgccccacg tgggacaaac ggagctgtcc acaatattct gtacgcatga
     3901 gatatccctt aacttagctg ccggcggatg ccccctgctg cgcaggtgac caagtcgcag
     3961 cgttagctgt atgtcacaac tgaatgaaaa aaggcggcta ttatactgga aattctgccg
     4021 caccgtaaga gcctggcccg cgctggaacg cctcgttacc actttatatc gggcctgaaa
     4081 tcagactcta cgccagtttg ctataaaggt gttgcccgaa ctcatacaaa ttaacaaaat
     4141 ttgtcgttcc gccatcggct aatcgcatta aggtgtgagg cacgattttg ttttttcagg
     4201 agtcatcatg cttgaactta atgctaaaac cactgcgctg gtggtgatcg atttacaaga
     4261 aggcatctta ccttttgccg gtggtccaca tactgccgat gaggtagtta atcgcgccgg
     4321 gaagctggcg gcgaaatttc gcgccagcgg tcagcccgtg tttctggtgc gcgttggctg
     4381 gtctgccgat tacgccgaag cattaaaaca gccggttgat gccccttccc ccgctaaagt
     4441 gttgcccgaa aactggtggc aacatccagc tgcattaggt gcaactgaca gtgatatcga
     4501 aataatcaaa cgtcagtggg gtgcgtttta cggtacggat ctggagttgc aattacgccg
     4561 ccgaggtatc gatacaatag tgttatgtgg gatctcgacc aatatcggag ttgaatccac
     4621 cgcccgcaat gcctgggaac ttggctttaa tctggtgatt gccgaagatg cctgtagtgc
     4681 cgctagcgcc gagcagcaca ataacagtat caaccatatc tacccgcgca tcgcccgtgt
     4741 gcgtagcgtg gaagagatcc tcaacgcgtt atgatttaca tcggtctacc gcaatggtcg
     4801 catcctaaat gggtgcggtt ggggatcacc agccttgaag agtatgcccg tcactttaac
     4861 tgcgtggagg gcaacaccac gctttacgcc ctgccgaaac ccgaggttgt cctgcgctgg
     4921 cgtgaacaga ccacagatga cttccgcttc tgttttaagt ttccggcgac catttcgcat
     4981 caggcagcat tacgacattg cgatgattta gtgactgaat ttttgacccg catgtcaccg
     5041 ttggctccgc gcattgggca atactggctg caactgcctg ccacattcgg cccacgggag
     5101 ctgcctgcgc tttggcattt tctcgattct ctccctggcg aatttaatta tggtgtggaa
     5161 gtccgccatc cacagttttt cgccaaaggg gaagaggaac aaacgcttaa tcgcggttta
     5221 catcagcgcg gcgttaatca ggtgatttta gacagccgcc cggttcatgc agcacgtcca
     5281 cacagtgaag ctattcgcga cgctcaacga aaaaaaccta aagttccggt acatgctgta
     5341 ctgacggcga caaatccgct gatccgtttt atcggtagtg atgatatgac gcaaaaccgg
     5401 gaattatttc aggtctggtt acaaaaatta gcgcagtggc atcagaccac tacgccttat
     5461 ctttttttac atacgccaga tattgcccag gccccggaac tggtacatac cctgtgggaa
     5521 gacttacgta aaacgcttcc agagatcgga gcagttccgg ctattccaca gcaatcttct
     5581 cttttctgaa tttgccacct atcatagaca ggtgccatcg gccattttaa agggagtttg
     5641 tatggtaagc gcgctgtatg ccgttttaag tgcgttgtta ttaatgaagt tctcttttga
     5701 tgtcgttcgc ctgcgaatgc agtaccgcgt tgcctatggt gacggtggtt ttagcgaact
     5761 gcaaagcgct attcgcattc atggtaacgc ggtggaatat attcccatcg cgattgtgct
     5821 gatgctgttt atggaaatga atggcgcaga aacctggatg gtgcatattt gcggcatcgt
     5881 tttgcttgct ggtcgtctga tgcattatta cggttttcat caccgtctgt tccgctggcg
     5941 acgttccggc atgagcgcca cctggtgtgc gctgttgctg atggtgctgg cgaatctttg
     6001 gtatatgccc tgggagttgg ttttctccct gcgttagcgc acaatacgcc actttctttt
     6061 tcccggattt ttacgttatg tctcaccgcg acacgctatt ttctgcccct atcgccagac
     6121 tgggcgactg gacctttgat gaacgggtag ctgaagtctt cccggatatg atccagcgtt
     6181 ccgttcccgg ctattccaat attatttcca tgattggtat gttagccgag cgcttcgttc
     6241 aacctggtac gcaggtttac gatctgggtt gttctctggg cgcggcgacg ctctcggtgc
     6301 gtcgcaacat tcatcatgat aattgcaaaa ttattgccat cgacaactcc ccggcgatga
     6361 ttgaacgctg ccgtcgtcat attgacgcct ataaagcccc tacgccagta gacgtcattg
     6421 aaggtgatat tcgcgatatc gccattgaaa acgcatcgat ggtggtgctg aattttaccc
     6481 tgcaattcct ggaaccttcc gagcgccagg cgttactgga taaaatttat caagggctga
     6541 accccggcgg cgcgctggtg ctttcggaaa aattcagttt cgaagatgcc aaagttggtg
     6601 aactgttgtt caacatgcac cacgacttta aacgtgccaa cggttacagc gaactggaga
     6661 tcagccagaa gcgcagcatg ctggaaaacg tgatgctgac cgattccgtg gaaacccata
     6721 aagcacgcct gcataaagcc ggttttgagc atagcgagct gtggttccag tgctttaact
     6781 ttggttcact ggtggcatta aaagcagagg acgctgcatg atcgactttg gtaactttta
     6841 ttctctgatt gccaaaaatc atctttcaca ctggctcgaa acgctgcccg cgcagattgc
     6901 taactggcag cgcgagcagc agcacgggtt gtttaagcag tggtcaaatg cggtggagtt
     6961 tctacctgaa attaaaccgt atcgtcttga tttattgcat agcgttacgg cagaaagcga
     7021 agagccactg agcgcagggc aaattaaacg cattgaaacg ctgatgcgca acctgatgcc
     7081 gtggcgcaaa gggccgttct cactgtatgg cgtcaacatc gataccgaat ggcgttccga
     7141 ctggaaatgg gatcgtgttc tgccccatct ttctgattta accggacgca ccattctgga
     7201 tgtcggctgc ggcagcggtt atcacatgtg gcgcatgatt ggcgcaggcg cgcacctcgc
     7261 ggtggggatc gaccctacgc aactgtttct gtgccagttt gaagcggtgc gtaaactact
     7321 gggtaatgat cagcgcgcgc atttgttacc gttaggtatt gaacaacttc cggcactgaa
     7381 agcctttgat accgtctttt cgatgggcgt gctttatcat cgtcgttcac cgctggagca
     7441 tctctggcag ttgaaagatc aactggtcaa tgaaggtgaa ctggtgcttg aaacgctggt
     7501 tattgatggc gatgagaaca cggtgctggt gccgggcgat cgttacgcgc aaatgcgtaa
     7561 tgtctatttc atcccttccg cgctggcgct gaaaaactgg ctgaagaagt gtggttttgt
     7621 agatatccgc attgcggatg tgagcgttac cactacagaa gagcagcgac gcactgaatg
     7681 gatggttacc gagtctctgt ccgatttcct cgacccacat gatccgagta agacagttga
     7741 aggttatcct gcgcctaaac gcgcggtgct gattgcgcgc aagccgtaaa ggtctggtat
     7801 tactgccgga tgcggcgtga acgccttatc cagcctacaa aaccttgcta attcaatata
     7861 ttgcagggac tatgtaggcc tgataagcat agcgcatcag gcagctttat gtttgcataa
     7921 cctcagcgcc cgtttccggg cgcttttcac gtcttatgcc tgaacagctg gctgatcaaa
     7981 cgccgttaac ttcggtgcgt tacccgtata tttttcgata tacaccagcg cagagttacc
     8041 ggcacaggca tttgccagct gcgagctggg gatatccgcc gttaacacgt tcgcactgcc
     8101 gtttttacac aagccatttt ccagatctgg ccatgcacct tcatgcacgc ataccacgcc
     8161 ttttttgatc ccgtcagtca ccacagcgcc tgtcaaaatc tgaccacgtt tgttccagac
     8221 acggaccaga tcgccattcg caataccaaa gcgagcagca tcttcggtgt gaatagtgat
     8281 tggttcacga tcggcaaccg catatttttt acgcagttgc gcatagttaa gctgactgtg
     8341 taaacggtgt gccggatgtg cggtcagaag ctgtaactgc ttctcgtcgg cggtaccttt
     8401 ccactcatca ggcgcaagcc aggttgggtg tgccgggcaa tccttatagc caaatttttc
     8461 cagcgttttg gagtaaatct caattttgcc gcttggcgta cccagcgcat ttttcaccgg
     8521 atcggcgcgg aaatcaccat aacgaacgta ctgttcgttc ttctcgctgc ggcgcatttc
     8581 gatcagttta ttttgctgcc agaaggcatt aaacattggc atagtgacgc gttgcgcacg
     8641 ggcacctttc tgagcggcat catagaaaaa tttcagccac gccatttcat ctttaccttc
     8701 ggtatagatc tcttttccgc ctggtttcag taattccgca agttcggcaa acacgtcaaa
     8761 atcgtttcgc gcttcaaatt gcggagcgac agcctgcttc atcggtacaa tatgctggtt
     8821 gctgtaatca ccggtcatcg tcaagtcatt gcgctcaaac gatgtggtga tcggtaatac
     8881 gatatctgca tgtttagcag ctgcggtcca gtagcattca gaaacgacga tcatctccgg
     8941 tttctgccat gctttgatca gacgattagt atcctggtga tgggtaaagt taccgccacc
     9001 cgcccaccag atcattttga tattcggata cgtttgttct ttaccgttat gctgatattt
     9061 gccgccagga ttttccagcg catcgacgat acgggcaaca ggaatcgccg tcattccgcc
     9121 atcatcagcg gcctcgctgg cctgtccggc aatcgccgca gacatttccg gcagcacgcc
     9181 gccaacacgc gtcgggttgc caccgttgga gtaatgataa gagaaaccaa aaccgccgcc
     9241 cggtgtacca atctgcccta acatcgccgc cagcgtcacc agcatccagt gtttctgttc
     9301 accatattgc tggcgctgaa ttccccagcc tgccatcagc attgtgcgat tcgccgccat
     9361 cagttcagcg agtttgacta tctgcgcttc cggtacgcca gtaatttccg ccgtccaggc
     9421 cgcgctcttc ggagtattat cgctcttacc tgtcagatac tcttcaaact gcggataccc
     9481 ggtggtgtat ttttcaagga acactttatc gtgtttgcct tgtgtcatca gggtatgcgc
     9541 aatccctaac atcagtgcca cgtcggtgcc catattcggc gcgatccagg tggcattata
     9601 gccaaagaac tcgatggttt cagagcggat aggatcaatg gcaatcactg gtttgccaga
     9661 ttttttcagc tgatggaagt attccagccc ttgctcatcg gtactgctcc aggcaatttt
     9721 taaggtattc agcgggttca ttccccacag caccacaacc tggctgtttt ccagaatcag
     9781 cggccaggag gtctgctgtt catacacctc tacagaaccg accacatgcg gcatgatcac
     9841 ctgtgctgcc ccggttgaat aatcgccgct atgcccggaa taaccgcccg ccaggttcat
     9901 ataacgttgc agtaaggttt gcgctttatg caacacgcca gaagagcgcc agccgtaaga
     9961 tccggcaaaa atggccgatg gtccgttagc tttacgaata cgctcatgtt gctcatgaat
    10021 cagttttaat gcctgttccc agctcacctg tacataggta tcttcgccac gacctttcgc
    10081 cggttgcagt ggattgtcga gatagctttt tctcaccatc ggatgctgaa tacgcgccgt
    10141 ggtgtgtacc tgatccgccg ccgtagactg taaggagttc ggtatggttt tcgccagcgc
    10201 gcctgtcgaa gaaacaatct tgccgtcctt cacttctacg ttcatcgctc cccaacgtcc
    10261 cgcggtgagg attttaccgc ccttctcttc tgcccatgcg ggtagcggtg ctgccgatgt
    10321 caccaccagc gctccagcgg caataccgct gtgtttaata aattcacgtc ttgttaatgt
    10381 cataacttcc tccctgatca acgaggatca ctgtttctcg gtaatatctt tggcgttgta
    10441 ctggaaatac cgcgttaaaa tgtccagttc gttttcgctc atgctggttc gtgcccccat
    10501 tcctttggca atggacggcc acgcattgac ggtgtaatgg tcggcggcaa taggggcatg
    10561 acaaccagcg caataggtat cgtcaagttt ttcagcgtat tgccatagca gtttacggtc
    10621 cgctaatgcg ggatcggtaa gcgcaccctg taaagacgcc tgacgccatt gattgccgta
    10681 ttcgtcagcc tgccattccc cgtttaccgt gagcgccttg ataccttctt cacttaatgt
    10741 ggctagcgcc agccgttgac ctgccgccag gtagagcgtg ttttcactgc cctgcatttg
    10801 ataaccctgc aacagaacga tcggctgttt gccactggca tcaacgacgg tgagatcggt
    10861 accaggattc acggtagcca gctcgcctat gcgagaagtt ttgaaaggat aaatatgtgc
    10921 gccattagtc actgaagtag cggcctgact ttccagctca tgcgccgcgt tgtcatccat
    10981 ttttatttct ggcggaaaat gggcaatgcc tttatgacaa tcgatacagg tttcgccgcc
    11041 cttttgtgct ttgttatgca ttttctgcgc agattcactt tgcgaggcaa tatccatggc
    11101 atcaaaagaa tggcaactac ggcacgttgc agagtcagtt gcttttaatt ctttccatac
    11161 tgtttcggcc atttcctggc gatgagcttc gaacttatcg tcactgtcta ttttgccgct
    11221 aacaaattca tgataaatat ctttagatgc ttttaattta gcgaataaat aatccatccc
    11281 tgactttgga atatggcaat cggcacattc cgcacgtatc cctttctggt tcgaaaagtg
    11341 gacagttccc tgatattcct caaaaggttt actc
//
LOCUS       DBFIFI010000136        11343 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.136, whole genome shotgun sequence.
ACCESSION   DBFIFI010000136 DBFIFI010000000
VERSION     DBFIFI010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11343
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.136"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            221..520
                     /gene="yoaC"
                     /locus_tag="N7B39_003395"
     CDS             221..520
                     /gene="yoaC"
                     /locus_tag="N7B39_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HGU2423316.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(640..819)
                     /gene="yoaH"
                     /locus_tag="N7B39_003396"
     CDS             complement(640..819)
                     /gene="yoaH"
                     /locus_tag="N7B39_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HGU2423317.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            893..2254
                     /gene="pabB"
                     /locus_tag="N7B39_003397"
     CDS             893..2254
                     /gene="pabB"
                     /locus_tag="N7B39_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HGU2423318.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRRVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPAPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2258..2836
                     /gene="nudL"
                     /locus_tag="N7B39_003398"
     CDS             2258..2836
                     /gene="nudL"
                     /locus_tag="N7B39_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HGU2423319.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            3020..4384
                     /gene="sdaA"
                     /locus_tag="N7B39_003399"
     CDS             3020..4384
                     /gene="sdaA"
                     /locus_tag="N7B39_003399"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HGU2423320.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     TANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4515..6113
                     /gene="pdeD"
                     /locus_tag="N7B39_003400"
     CDS             4515..6113
                     /gene="pdeD"
                     /locus_tag="N7B39_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HGU2423321.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTTDRVLIKGSPVLIQWTPAASSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFSFTINVNGPGAPALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(6117..7673)
                     /gene="yoaE"
                     /locus_tag="N7B39_003401"
     CDS             complement(6117..7673)
                     /gene="yoaE"
                     /locus_tag="N7B39_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HGU2423322.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(7661..7819)
                     /gene="yoaL"
                     /locus_tag="N7B39_003402"
     CDS             complement(7661..7819)
                     /gene="yoaL"
                     /locus_tag="N7B39_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HGU2423323.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            8136..9107
                     /gene="manX"
                     /locus_tag="N7B39_003403"
     CDS             8136..9107
                     /gene="manX"
                     /locus_tag="N7B39_003403"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HGU2423324.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            9170..9970
                     /gene="manY"
                     /locus_tag="N7B39_003404"
     CDS             9170..9970
                     /gene="manY"
                     /locus_tag="N7B39_003404"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HGU2423325.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            9983..10834
                     /gene="manZ"
                     /locus_tag="N7B39_003405"
     CDS             9983..10834
                     /gene="manZ"
                     /locus_tag="N7B39_003405"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HGU2423326.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10889..>11343
                     /gene="yobD"
                     /locus_tag="N7B39_003406"
     CDS             10889..>11343
                     /gene="yobD"
                     /locus_tag="N7B39_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HGU2423327.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLERIYKLLVST"
BASE COUNT         2783 a         2865 c         2853 g         2842 t
ORIGIN      
        1 ctggttttct ttcttagctg aacaatctgg ttatagcgtg cggactgacc gttaattttc
       61 tccatcttac tggataacac accatgagct gctaccttta aaacacctga atatcaacag
      121 gttaactcgt gccatatcgt tataatccct ttgatattag atgcaaatta aggtcatata
      181 tagccttatc gataacatgg ttaattttaa ggataagaat atgcctgctg taatagataa
      241 agccctggat ttcattggtg ccatggatgt atcagcgcca acaccaagtt cgatgaatga
      301 aagcacggcg aagggaatct ttaaatattt aaaagaactg ggagtacccg cgagtgccgc
      361 tgatattacg gcgcgagctg accaggaagg ctggaaccct gggttcacgg aaaaaatggt
      421 tggatgggca aaaaaaatgg agacaggtga acgctctgtg attaaaaatc ctgagtactt
      481 ttcaacatat atgcaggaag aactaaaagc actggtctga gttaaattta tatcagcata
      541 aatgggtcag gacgctttta atcacatata aaaagcgtcc tttttctcac aatcaaataa
      601 tcatcagctt ctataacgaa atcgatacgg attagtgtgt tactcgtctt catcctcaaa
      661 acgcgccacg attcgctcgc cggagtggtt ggcacgcagc tcttctgcca ccagcgcgat
      721 tgcctggccg ctgctcatcc cttgtgccat cagttcctgg atccgctcga cagctttttg
      781 ctgctgttca tgggtgagtg aaggtaaacc tgcaaacatt gttaactcct gctaaattgt
      841 tggcgctaat tatttcatgc tacccggcac atagccagta gagtcaggac tgatgaagac
      901 gttatctccc gctgtgatta ctttaccctg gcgtcaggac gccgctgaat tttatttctc
      961 ccgcttaagc cacctgccgt gggcgatgct tttacactcc ggctatgccg atcatccgta
     1021 tagccgcttt gatattgtgg tcgccgatcc gatttgcact ttaaccactt taggtaaaga
     1081 aaccgttgtt agtgaaagcg aaaaacgcac aacgaccact gatgacccgc tacaggtgct
     1141 ccagcaggtg ctggatcgcg cagacattcg cccaacgcat aacgaagatt tgccatttca
     1201 gggcggcgca ctggggttgt ttggctacga tctgggccgc cgttttgagt cactgccaga
     1261 aattgcggaa caagatatcg ttctgccgga tatggcagtg ggtatctacg attgggcgct
     1321 cattgtcgac caccagcgtc atacagtttc tttgctgagt cataatgatg tcaatgcccg
     1381 tcgggcctgg ctggaaagcc agcaattctc accgcaggaa gatttcacgc tcacttccga
     1441 ctggcaatcc aatatgaccc gcgagcagta cggcgaaaaa tttcgccggg tacaggaata
     1501 tctgcacagc ggtgattgct atcaggtgaa tctcgcccag cgttttcatg cgacctattc
     1561 tggcgatgaa tggcaggcat tccttcagct taatcaggcc aaccgcgcgc catttagcgc
     1621 ttttttacgt cttgaacagg gtgcaatttt aagcctttcg ccagagcggt ttattctttg
     1681 tgataatagt gaaatccaga cccgcccgat taaaggcacg ctaccacgcc tgcccgctcc
     1741 tcaggaagat agcaaacaag cagaaaaact ggcgaactca gcgaaagatc gtgccgaaaa
     1801 tctgatgatt gtcgatttaa tgcgtaatga tatcggtcgt gttgccgtag caggttcggt
     1861 aaaagtacca gagctgttcg tggtggaacc cttccctgcc gtgcatcatc tggtcagcac
     1921 cataacggcg caactaccag aacagttaca cgccagcgat ctgctgcgcg cagcttttcc
     1981 tggtggctca ataaccgggg ctccgaaagt acgggctatg gaaattatcg acgaactgga
     2041 accgcagcga cgtaatgcct ggtgcggcag cattggctat ttgagctttt gcggcaacat
     2101 ggataccagc attactatcc gcacgctgac tgccattaac ggacaaatat actgctctgc
     2161 gggaggtgga attgtcgccg atagccagga agaagcggaa tatcaggaaa cttttgataa
     2221 agttaataag atattacgcc aactggagaa gtaagacgtg gaataccgta gcctgacgct
     2281 tgatgatttt ttatcgcgct ttcaactttt gcgcccgcaa attaaccggg aaaccctaaa
     2341 tcatcgtcag gctgccgtgt taatccccat cgtccgtcga ccgcaaccgg ggttgttgct
     2401 gactcagcgt tcaattcatc tgcgtaaaca cgctggacaa gtggcattcc ctggaggtgc
     2461 agtcgatgac acggacgcat cagttatcgc cgccgcgctg cgcgaagctg aagaagaggt
     2521 tgctataccg ccttccgccg ttgaagttat cggcgtgctg ccgcccgtcg atagcgtaac
     2581 tggctaccag gtaaccccag tggtcggcat tatcccgccc gatctgccgt atcgcgccag
     2641 tgaagatgaa gtttccgctg tgtttgaaat gccgctcgcc caggcattac atctgggtcg
     2701 ttatcaccct ttagatatct accgccgcgg cgattcacat cgggtatggc tatcctggta
     2761 cgaacagtat tttgtatggg gaatgaccgc aggcataatt cgtgagctgg cgctgcaaat
     2821 tggtgtgaaa ccctgactat acttatcttt acatctacaa aacactactt gagacaatca
     2881 tcgcaatatt agttaaatcg cggtttttga ttagtttaat tcatgtgaat agttaagcca
     2941 gtcgccgcgt tccctcttac actatgcgct gttattagtt cgttactgga agtccagtca
     3001 ccttgtcagg agtattatcg tgattagtct attcgacatg tttaaggtgg ggattggtcc
     3061 ctcatcttcc cataccgtag ggcctatgaa ggcgggtaaa cagttcgtcg atgatctggt
     3121 cgaaaaaggc ttactggata gcgttactcg cgttgccgtg gacgtttatg gttcactgtc
     3181 gctgacgggt aaaggccacc acaccgatat cgccattatt atgggtcttg caggtaacga
     3241 acctgccacc gtggatatcg acagtattcc cggttttatt cgcgacgtag aagagcgcga
     3301 acgtctgctg ctggcacagg gacggcatga agtggatttc ccgcgcgaca acgggatgcg
     3361 ttttcataac ggcaacctgc cgctgcatga aaacggtatg caaatccacg cctataacgg
     3421 cgatgaagtc gtctacagca aaacttatta ttccatcggc ggcggtttta tcgtcgatga
     3481 agaacacttt ggtcaggata ctgccaacga agtgagcgtg ccgtatccgt tcaaatctgc
     3541 caccgaactg ctcgcgtact gtaatgaaac cggctattcg ctgtctggtc tcgctatgca
     3601 gaacgaactg gcgctgcaca gcaagaaaga gatcgacgag tatttcgcgc atgtctggca
     3661 aaccatgcag gcatgtatcg atcgcgggat gaacaccgaa ggtgtactgc caggcccgct
     3721 gcgcgtgcca cgtcgtgcgt ctgccctgcg ccggatgctg gtttccagcg ataaactgtc
     3781 taacgatccg atgaatgtca ttgactgggt aaacatgttt gcgctggcag ttaacgaaga
     3841 aaacgccgcc ggtggtcgtg tggtaactgc gccaaccaac ggtgcctgcg gtatcgttcc
     3901 ggcagtgctg gcttactatg accactttat tgaatcggtc agcccggaca tctatacccg
     3961 ttactttatg gcagcgggcg cgattggtgc attgtataaa atgaacgcct ctatttccgg
     4021 tgcggaagtt ggttgccagg gcgaagtggg tgttgcctgt tcaatggctg ctgcgggtct
     4081 tgcagaactg ctgggcggta gcccggaaca ggtttgcgtg gcggcggaaa ttggcatgga
     4141 acacaacctt ggtttaacct gcgacccggt tgcagggcag gttcaggtgc cgtgcattga
     4201 gcgtaatgcc attgcctctg tgaaggcgat taacgccgca cggatggctc tgcgccgcac
     4261 cagtgcaccg cgcgtctcgc tggataaggt catcgaaacg atgtacgaaa ccggtaagga
     4321 catgaacgcc aaataccgcg aaacctcacg cggtggtctg gcaatcaaag tccagtgtga
     4381 ctaatacttc ttactcgccc atctgcaacg gatgggcgaa tttatccccg ctttctcgtc
     4441 tgctgtaata ttccccacta cacttccact gttgcgtcag gcgtttgtcg ccatacgctt
     4501 acagagtggc ccgcatgcaa aaagcacaac ggatcattaa aacctatcgc cgtaatcgaa
     4561 tgattgtttg tacgatttgc gcactcgtta cgctcgcttc gaccctgagc gtgcgattta
     4621 tttcacagcg taacttaaat caacaacggg tagtacaatt cgccaatcac gctgtagagg
     4681 aattagataa agtactgctt cccctacagg caggtagcga agtcttgctt ccgctgattg
     4741 gtctgccctg ctctgtcgcc catttgccat tacgtaaaca ggcggcaaaa ctccaaactg
     4801 tgcgatccat tggcctggtg caagacggca cactttattg ctccagcatt tttggttatc
     4861 gcaatgtgcc cgtcgtggac attctggctg aacttcctgc accgcaacca cttttacgcc
     4921 tgacgaccga ccgtgtcctg attaaaggca gtccggtttt gattcaatgg acgcctgcag
     4981 cgagcagtag caatgctggg gtcatggaga tgattaacat cgacttactg acggcaatgc
     5041 tgcttgagcc acaactgccg caaatcagta gcgccagcct gacggtggac aaacggcatt
     5101 tgctctatgg taatgggctg gtagattccc ttccacaacc tgaagacaat gaaaactatc
     5161 aggtttcttc gcaacgcttt tcttttacca ttaacgttaa tggtccgggg gctccggcgc
     5221 tggcatggca ctatcttcca acacaattac cgctggcggt actgctaagt ttactggtgg
     5281 gctacatcgc ctggctggcg accgcttacc gaatgagctt ttcccgcgaa atcaatctgg
     5341 gcctggcgca acatgagttc gaattgttct gtcagccttt gcttaatgcg cgcagccagc
     5401 aatgtattgg tgtagagatt ttgctgcgct ggaacaatcc gcgtcagggc tggatttcac
     5461 cggatgtgtt tattcctatc gcggaagaac atcatttaat tgtgccactg acccgctatg
     5521 tgatggcaga aaccattcgt cagcgccatg ttttcccgat gagtagtcag tttcatgttg
     5581 gcattaacgt cgcacccagc cattttcgcc gtggtgtgct gataaaagat ctcaatcagt
     5641 actggtttag cgctcacccg attcagcaac tgatcctcga aatcaccgaa cgcgatgcct
     5701 tactggatgt tgattatcgg attgcccgcg agctacatcg taaaaacgtc aaactggcga
     5761 ttgatgactt cggcaccggc aacagttcat tttcctggct tgaaacatta cgtcctgacg
     5821 tgctgaaaat tgataagtca tttaccgcag ctataggttc tgacgcggtt aactcgacgg
     5881 tgaccgatat catcatcgcg ctggggcaaa gactgaatat tgaactggtg gcggagggcg
     5941 tggaaacgca agaacaggcg aagtatttgc gccgtcatgg cgtgcatatt ttgcaagggt
     6001 atttgtacgc acagccgatg ccgctacgtg attttcccaa atggctggcg ggcagccaac
     6061 cgccgcccgc ccggcataat ggacatatca cgcccattat gccgttacgt taacgcttac
     6121 tcatcttcat cgtgcgccgg ttgctcttta acaatgcgaa ccagatcaac acgataatca
     6181 ttggcttcaa tgatggtgat atgcagtggc cctacatcaa tcacatcgcc cacacgggga
     6241 atgtgaccat ttgccgagat cacgaggccc gcgaccgtcg cgatatcatc gtcatcggca
     6301 aggtgctcaa catcaagcgc ctgctgcaag gcatgcaaat ctgtaccgcc ttttaccagc
     6361 cagccgtcac catcagtaat gatttccggc gtttcgtcag cgtccgggaa ttcacccgca
     6421 atggcttcca gcacatccag cggcgtgacc aagccttgta ccacaccaaa ctcgttggtc
     6481 acgataacaa agctcccgcg agcacgacgc agcacgccca gcaggttgat cggatcgagg
     6541 gtttcaggga cgataatcgc cggagacgcc gaagcaatcg ccgccacatc aacgccctct
     6601 tccagcgcca ccagcagttc tttagcacgt acgattccga tgatttcatc cagttcaccg
     6661 cgacataccg ggaacaggct gtgcggtgag gagagcagct gctcgcggat ttcatcgacc
     6721 ccgagattag cgtcaaccca gcttatttca ccgcgcggcg tcatgatccc gcgcagagaa
     6781 cgcgacgcca gcgtcagtac gccgttaatc atgtaacgtt cttcttcggc aaatgcacct
     6841 tccgggatcg gcatcggcat cgggttatcg gcatcgtgct gaacattggc ctgacgtttc
     6901 ccgcccatca aacgcaggat ggcatcggca gtacgcgctc gcagcggcaa agtcgactgg
     6961 tgacgaataa agttgcgacg cgcaatctgg ttaaacactt cgatgatgat cgagaagcca
     7021 atcgcggcat acaggtaacc tttcggaatg tggaaaccga aaccttctgc caccagactc
     7081 agaccaatca ttaacaggaa gctcagacag agcaccacca ccgtggggtg ctggttaacg
     7141 aatcgcgtca gcggtttgga tgccagcaac ataaccgcca tcgcaatcac taccgccgcc
     7201 atcatcaccg gcagatggtt aaccatccct actgcagtaa ttaccgcatc caacgagaag
     7261 acggcgtcaa ggatgacaat ctgtgtgacg accacccaga aactggcgta gcctttaccg
     7321 tggccggaat catgatcgcg gttttccagc cgttcatgca gttcggttgt tgctttgaac
     7381 agcaagaata tccccccgaa caacataatc aggtcgcgtc cggagaagga gaaatccatg
     7441 acggtaaata gcggtttggt cagcgtgacc atccatgaaa tcagcgacag cagccccaga
     7501 cgcataatca gcgccagtga taaccccagc aaacgcgctt tatcgcgttg ttttggcggc
     7561 agtttgtcag caagaatggc gatgaagacc aggttatcga tacccagcac aatttcgaga
     7621 acaacaagcg tgagtagccc cgcccaaatt gaggggtcca ttaagaattc catgacaagc
     7681 tcctgcttaa ggaatagcta ttcgacgcca gaaataatgc aggcgtaacg acaaaatgca
     7741 aacgaaaggt gcggcataga gtgccagaaa ggcaggcgtt aaaaggcctg atgctgaaat
     7801 gacgtcggtg acgatccata ctgcgggcta ctgccctata ctccatgatt gttaaacggg
     7861 agttaaacat atcagagacg cctctgattt ggcaaagatt taccttcctt tgcaaacgaa
     7921 tgtgacaagg atattttacc tttcgaaatt tctgctaatc gaaagttaaa ttacggatct
     7981 tcatcacata aaataatttt ttcgatatct aaaataaatc gcgaaacgca ggggtttttg
     8041 gttgtagccc ttatctgaat cgattcgatt gtggacgacg attcaaaaat acatctggca
     8101 cgttgaggtg ttaacgataa taaaggaggt agcaagtgac cattgctatt gttataggca
     8161 cacatggttg ggctgcagag cagttgctta aaacggcaga aatgctgtta ggcgagcagg
     8221 aaaacgtcgg ctggatcgat ttcgttccag gtgaaaatgc cgaaacgctg attgaaaagt
     8281 acaacgctca gttggcaaaa ctcgacacca ctaaaggcgt gctgtttctc gttgatacat
     8341 ggggaggcag cccgttcaat gctgccagcc gcattgtcgt cgacaaagag cattatgaag
     8401 tcattgcagg cgttaacatt ccaatgctcg tggaaacgtt aatggctcgt gatgatgacc
     8461 caagctttga tgaactggtt gcgctggcag tagaaacagg ccgtgaaggc gtgaaagcac
     8521 tgaaagccaa accggttgaa aaagccgcgc cagcacccgc tgccgcagca cccaaagcgg
     8581 ctccaactcc ggcaaaacca atggggccaa acgactacat ggttattggc cttgcgcgta
     8641 tcgacgaccg tctgattcac ggtcaggtcg ccacccgctg gaccaaagaa accaatgtct
     8701 cccgtattat tgttgttagt gatgaagtgg ctgcggatac tgttcgtaag acactgctca
     8761 cccaggttgc acctccgggc gtaacagcac acgtagttga tgttgccaaa atgattcgcg
     8821 tctacaacaa cccgaaatat gctggcgaac gtgtaatgct gttatttacc aacccaacag
     8881 atgtagagcg tctcgttgaa ggcggcgtga aaatcacctc tgttaacgtc ggtggtatgg
     8941 cattccgtca gggtaaaacc caagtgaata acgcggtttc ggttgatgaa aaagatatcg
     9001 aggcgttcaa aaaactgaat gcacgcggta ttgagctgga agtccgtaag gtttccaccg
     9061 atccgaaact gaaaatgatg gatctgatca gcaaaatcga taagtaacgt attgtgttga
     9121 ttatcagtca gttttcacac tttagtcata cgtaaacagg agaagtacaa tggagattac
     9181 cactcttcaa attgtgctgg tatttatcgt agcctgtatc gcaggtatgg gatcaatcct
     9241 cgatgaattt cagtttcacc gtccgctaat cgcgtgtacc ctggtgggta tcgttcttgg
     9301 ggatatgaaa accggtatta ttatcggtgg tacgctggaa atgatcgcgc tgggctggat
     9361 gaacatcggt gctgcagttg cgcctgacgc cgctctggca tctatcattt ctaccattct
     9421 ggttatcgca ggtcatcaga gcattggtgc aggtattgca ctggcaatcc ctctggccgc
     9481 tgcgggccag gtactgacca tcatcgttcg tactattacc gttgctttcc agcacgctgc
     9541 ggataaggct gctgataacg gcaacctgac agcgatttcc tggatccacg tttcttctct
     9601 gttcctgcaa gcaatgcgtg tggctattcc ggccgtcatc gttgcgctgt ctgtaggtac
     9661 cagcgaagta cagaacatgc tgaatgcgat tccggaagtg gtgaccaatg gtctgaatat
     9721 cgccggtggc atgatcgtgg tggttggtta tgcgatggtt atcaacatga tgcgtgctgg
     9781 ctacctgatg ccgttcttct acctcggctt cgtaaccgca gcattcacca actttaacct
     9841 ggttgctctg ggtgtgattg gtactgttat ggcagtgctc tacatccaac ttagcccgaa
     9901 atacaaccgc gtagccggtg cgcctgctca ggcagctggt aacaacgatc tcgataacga
     9961 actggactaa caggtgagcg aaatggttga tacaactcaa actaccaccg agaaaaaact
    10021 cactcaaagt gatattcgtg gcgtcttcct gcgttctaac ctcttccagg gttcatggaa
    10081 cttcgaacgt atgcaggcac tgggtttctg cttctctatg gtaccggcaa ttcgtcgcct
    10141 ctaccctgag aacaacgaag ctcgtaaaca agctattcgc cgtcacctgg agttctttaa
    10201 cacccagccg ttcgtggctg cgccgattct cggcgtaacc ctggcgctgg aagaacagcg
    10261 tgctaatggc gcagagatcg acgacggtgc tatcaacggt atcaaagtcg gtttgatggg
    10321 gccgctggct ggtgtaggcg acccgatctt ctggggaacc gtacgtccgg tatttgcagc
    10381 actgggtgcc ggtatcgcga tgagcggcag cctgttaggt ccgctgctgt tcttcatcct
    10441 gtttaacctg gtgcgtctgg caacccgtta ctacggcgta gcgtatggtt actccaaagg
    10501 tatcgatatc gttaaagata tgggtggtgg cttcctgcaa aaactgacgg aaggggcgtc
    10561 tatcctcggc ctgtttgtca tgggggcatt ggttaacaag tggacacatg tcaacatccc
    10621 gctggttgtc tctcgcatta ctgaccagac gggcaaagaa cacgttacta ctgtccagac
    10681 tattctggac cagttaatgc caggcctggt accactgctg ctgacctttg cttgtatgtg
    10741 gctactgcgc aaaaaagtta acccgctgtg gatcatcgtt ggcttcttcg tcatcggtat
    10801 cgctggttac gcttgcggcc tgctgggact gtaagactgt tgtacactac cggggccttt
    10861 tggccccgtt tttttatctg gaggattaat gacaatcacg gacctggtac tgattctttt
    10921 catcgccgca ctcctggcct tcgcgatcta cgatcagttc atcatgcccc gccgtaacgg
    10981 ccccaccctg ctggcaattc ctttgctccg gcgtggtcgc atcgatagcg ttatcttcgt
    11041 cggattgatt gtcattctta tctataacaa cgtcacgaat catggtgcgt taataacgac
    11101 atggttatta agcgcactgg ctctgatggg tttttatata ttctggatcc gcgttccgaa
    11161 gatcatcttt aaacaaaaag gttttttctt cgccaatgtc tggattgaat atagccgaat
    11221 caaagcgatg aacttgtcgg aagatggcgt gttggtgatg caattagaac agcgtcggct
    11281 gttaatccgc gttcgaaata tcgacgatct ggaaagaatt tataaacttc tcgtttcaac
    11341 tca
//
LOCUS       DBFIFI010000137        11320 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.137, whole genome shotgun sequence.
ACCESSION   DBFIFI010000137 DBFIFI010000000
VERSION     DBFIFI010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11320
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.137"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..135
                     /gene="hdeB"
                     /locus_tag="N7B39_003407"
     CDS             <1..135
                     /gene="hdeB"
                     /locus_tag="N7B39_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HGU2423328.1"
                     /translation="KGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQASNDLPN
                     "
     gene            199..846
                     /gene="yhiD"
                     /locus_tag="N7B39_003408"
     CDS             199..846
                     /gene="yhiD"
                     /locus_tag="N7B39_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HGU2423329.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(898..1668)
                     /locus_tag="N7B39_003409"
     CDS             complement(898..1668)
                     /locus_tag="N7B39_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HGU2423330.1"
                     /translation="MISAQNLVYSLQGRRLTDNVSLTFPGGEIVAILGPNGAGKSTLL
                     RQLTGYLQPDSGECRLFNKPLNEWSITELAKHRAVMRQNSHMAFPFSVQEVIQMGRHP
                     HRTGNQDNETAQIMALCDCQALANRDYRQLSGGEQQRVQLARLLVQLWEPTPSPKWLF
                     LDEPTSALDIHHQQHLFRLLRQLVHERQFNVCCILHDLNLAAHYADRVVLMQKGKVIA
                     NGKPQDVLTQQALTMLYGADITVLKDPANHSPLIVLDH"
     gene            complement(1665..2657)
                     /gene="shuU"
                     /locus_tag="N7B39_003410"
                     /gene_synonym="chuU"
     CDS             complement(1665..2657)
                     /gene="shuU"
                     /locus_tag="N7B39_003410"
                     /gene_synonym="chuU"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease ShuU/ChuU"
                     /protein_id="HGU2423331.1"
                     /translation="MLKDSFSSARVFMGLSLLLLALVLFGASQGALKISFDALFDEEY
                     RDIWLNIRLPRVLLAVLVGAALATAGVIMQGLFRNPMADPGLLGVSSGSALMVGVAIV
                     LPFSFPVVLVLYEQMVFAIAGSLVVCAIIFLITQRHRDGSMMQLLLAGIAINALCGAA
                     IGILSYIGDEPQLRQLTLWMMGNLGQAQWPTLLVASSFILPAIIATTCLAGTLNLLQL
                     GDEEAHYLGVNVKRKRQQLLLVSSLLVGAAVSVSGIIGFIGLVIPHLIRMTTGANHRW
                     LIPCSALAGACLLLMADTLARTLVQPAEMPVGLLTSLLGGPYFMWLILRNRRIT"
     gene            complement(2706..3329)
                     /gene="chuY"
                     /locus_tag="N7B39_003411"
     CDS             complement(2706..3329)
                     /gene="chuY"
                     /locus_tag="N7B39_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobilin reductase"
                     /protein_id="HGU2423332.1"
                     /translation="MTPWLLFGAGGKGVGARTLELALAEQRPVVAVIRHADAATKLAQ
                     QGVQVFTGDACDASVVAAACRAAGPDALIISTMGGAQDYLAHRTVIDEAEKAGISRMI
                     LVTSLGCGDSWPFLSERAKAAFGQAVREKTLAESWLQTSQLDYAILRPGGLLDGAATG
                     KAQRIQNQECHGFVRRADVAAHIHELANAPALNQQVYSLIEPDLKPA"
     gene            complement(3329..3823)
                     /gene="hutX"
                     /locus_tag="N7B39_003412"
     CDS             complement(3329..3823)
                     /gene="hutX"
                     /locus_tag="N7B39_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization cystosolic carrier protein
                     HutX"
                     /protein_id="HGU2423333.1"
                     /translation="MSHVSLQEFLKTEPDGTLEVVAEQYNTTLLEVVRNLPSSTVVPG
                     DKFDTVWDTVCEWGNVTTLVHTADVILEFSGELPSGFHRHGYFNLRGKHGMSGHIKAE
                     NCTHIALIERKFMGMDTASILFFNKEGSAMLKIFLGRDDHRQLLSEQVSAFHTLAASL
                     KEHA"
     gene            complement(3836..5173)
                     /gene="hutW"
                     /locus_tag="N7B39_003413"
     CDS             complement(3836..5173)
                     /gene="hutW"
                     /locus_tag="N7B39_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme anaerobic degradation radical SAM
                     methyltransferase ChuW/HutW"
                     /protein_id="HGU2423334.1"
                     /translation="MNANNTLDLTPHFALDGDQPFKDRRAMMPFRGAIPVAKEQLAQT
                     WQEMINQTASPRKRLVYLHIPFCATHCTFCGFYQNRFNEDACAHYTDALIREIEMEAD
                     SVLHQSAPIHAVYFGGGTPSALSAHDLARIITTLREKLPLAPDCEITIEGRVLNFDAE
                     RIDACLDAGANRFSIGIQSFNSKIRKKMARTSDGPTAIAFMESLVKRDRAAVVCDLLF
                     GLPGQDAQTWGEDLAIARDIGLDGVDLYALNVLSNTPLGKAVENGRTTVPSPAERRDL
                     YLQGCDFMDDAGWRCISNSHWGRTTRERNLYNLLIKQGADCLAFGSGAGGSINGYSWM
                     NERNLQTWHESVAAGKKPLMLIMRNAERNAQWRHTLQSGVETARVPLDELTPHAEKLA
                     PLLAQWHQKGLSRDASTCLRLTNEGRFWASNILQSLNELIQVLNAPAIMREKP"
     gene            complement(5193..6107)
                     /gene="shuT"
                     /locus_tag="N7B39_003414"
     CDS             complement(5193..6107)
                     /gene="shuT"
                     /locus_tag="N7B39_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     ShuT"
                     /protein_id="HGU2423335.1"
                     /translation="MPRIITRPFLFSPLTLCISAVASAAKTAVKRKKLFTAVLALSWA
                     FSVTAAERIVVAGGSLTELIYAMGAGERVVGVDETTSYPPETAKLPHIGYWKQLSSEG
                     ILSLRPDSVITWQDAGPQIVLDQLRAQKVNVVTLPRVPATLQQMYANIRQLAKTLQVP
                     EQGEALVTQISQRLERVQQNVATKKAPVKAMFILSAGGSAPQVAGKGSVADAILSLAG
                     AENVATHQQYKSYSAESLIAANPEVIVVTSQMVDGDINRLRSIAGITHTAAWKNQRII
                     TVDQNLILGMGPRIADVVESLHQQLWPQ"
     gene            6790..8772
                     /gene="chuA"
                     /locus_tag="N7B39_003415"
                     /gene_synonym="shuA"
     CDS             6790..8772
                     /gene="chuA"
                     /locus_tag="N7B39_003415"
                     /gene_synonym="shuA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent heme/hemoglobin receptor
                     ChuA/ShuA"
                     /protein_id="HGU2423336.1"
                     /translation="MSRPQFTSLRLSLLALAVSATLPTFAFATETMTVTATGNARSSF
                     EAPMMVSVIDTSAPENQTATSATDLLRHVPGITLDGTGRTNGQDVNMRGYDHRGVLVL
                     VDGVRQGTDTGHLNGTFLDPALIKRVEIVRGPSALLYGSGALGGVISYDTVDAKDLLQ
                     EGQSSGFRVFGTGGTGDHSLGLGASAFGRTENLDGIVAWSSRDRGDLRQSNGETAPND
                     ESINNMLAKGTWQIDSAQSLSGLVRYYNNDAREPKNPQTVEASESSNPMVDRSTIQRD
                     AQLSYKLAPQGNDWLNADAKIYWSEVRINAQNTGSSGEYREQITKGARLENRSTLFAD
                     SFASHLLTYGGEYYRQEQHPGGATTGFPQAKIDFSSGWLQDEITLRDLPITLLGGTRY
                     DSYRGSSDGYKDVDADKWSSRAGMTINPTNWLMLFGSYAQAFRAPTMGEMYNDSKHFS
                     IGRFYTNYWVPNPNLRPETNETQEYGFGLRFDDLMLSNDALEFKASYFDTKAKDYIST
                     TVDFAAATTMSYNVPNAKIWGWDVMTKYTTDLFSLDVAYNRTRGKDTDTGEYISSINP
                     DTVTSTLNIPIAHSGFSVGWVGTFADRSTHISSSYSKQPGYGVNDFYVSYQGQQALKG
                     MTTTLVLGNAFDKEYWSPQGIPQDGRNGKIFVSYQW"
     gene            8821..9849
                     /gene="chuS"
                     /locus_tag="N7B39_003416"
     CDS             8821..9849
                     /gene="chuS"
                     /locus_tag="N7B39_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hematinate-forming heme oxygenase ChuS"
                     /protein_id="HGU2423337.1"
                     /translation="MNHYTRWLELKEQNPGKYARDIAGLMNIREAELAFARVTHDAWR
                     MHGDIREILAALESVGETKCICRNEYAVHEQVGTFTNQHLNGHAGLILNPRALDLRLF
                     LNQWASVFHIKENTARGERQSIQFFDHQGDALLKVYATDNTDMAAWSELLARFITDEN
                     TPLELKAVDAPVVQTRADATVVEQEWRAMTDVHQFFTLLKRHNLTRQQAFNLVADDLA
                     CKVSNSALAQILESAQQDGNEIMVFVGNRGCVQIFTGVVEKVVPMKGWLNIFNPTFTL
                     HLLEESIAEAWVTRKPTSDGYVTSLELFAHDGTQIAQLYGQRTEGEQEQAQWRKQIAS
                     LIPEGVAA"
     gene            complement(9910..10440)
                     /gene="dctR"
                     /locus_tag="N7B39_003417"
     CDS             complement(9910..10440)
                     /gene="dctR"
                     /locus_tag="N7B39_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HGU2423338.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(10596..11162)
                     /gene="slp"
                     /locus_tag="N7B39_003418"
     CDS             complement(10596..11162)
                     /gene="slp"
                     /locus_tag="N7B39_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HGU2423339.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT         2911 a         2672 c         2825 g         2912 t
ORIGIN      
        1 aaaggtggcg atacagttac tttaaatgaa accgatctca ctcaaattcc taaagtgatc
       61 gaatactgta agaaaaaccc gcagaaaaat ttgtatacct tcaaaaatca agcatctaat
      121 gacttgccga attaatgagg tacaagtaaa aaggagtggc aagttgagcc atcttgctgc
      181 tcctttttgc atttttatat gacagcagaa tttattatac gtcttatact cgcggcaatt
      241 gcctgtggcg ctattggcat ggaaaggcaa atgcgcggca aaggagcagg gttacgcact
      301 catgtattaa ttggcatggg aagcgcactg tttatgattg tttcgaaata tggttttgct
      361 gacgtgctgt ctttagatca cgtcggactg gaccccagcc gtattgctgc tcaggtggtg
      421 acgggcgtcg ggtttatcgg tgcaggtaac attctcgttc gtaaccaaaa tattgtaggt
      481 ttgacgacgg cagcggatat ctgggtgacc gccgccatag gtatggttat tggcagtggt
      541 atgtacgaac tgggtattta tggttcagtg atgaccttgc tggtgctgga agtcttccat
      601 caattaacct tccgcctgat gaacaaaaat taccatttac agctgacatt agtgaacggt
      661 aatacggtat caatgctcga ctggtttaag cagcaaaaaa taaaaactga tttagtttcg
      721 ttgcaggaaa atgaagatca tgaagtggtc gccattgata tccagcttca tgccacaacg
      781 tcgatagaag atctgttgcg attattaaaa ggtatggcag gggtgaaagg cgtttcaatt
      841 agctaatctg tatagttatt tattcgcagg agtaccagtg cggtactcct gttgctttta
      901 atgatcgagg acgatcagtg gtgaatggtt ggcaggatct ttaagtacgg taatatccgc
      961 gccgtagagc attgtcagcg cctgctgtgt caatacgtcc tgtggtttac catttgcgat
     1021 aacctttcct ttttgcatta atacaacacg gtctgcataa tgcgcggcaa gatttagatc
     1081 gtgcaatatg cagcagacat tgaactgccg ctcgtgaact aactggcgta ataaacgaaa
     1141 caagtgctgc tgatggtgaa tatccagcgc cgaagttggt tcatcaagaa ataaccattt
     1201 cggtgaggga gttggttccc agagttgcac cagcaggcgc gctaattgca cgcgctgttg
     1261 ttcaccaccg gataattgcc gatagtcgcg gtttgctaac gcctgacagt cacataacgc
     1321 cattatttgt gctgtttcat tatcctgatt accagtacga tgcggatgcc gacccatctg
     1381 gataacttct tgtacgctga atggaaacgc catgtggcta ttttgacgca tcacggcccg
     1441 gtgtttcgcc agttcagtaa tcgaccattc atttaatggc ttattaaaca accgacattc
     1501 gccgctatca ggttgaagat agccggttaa ctggcgaagc agcgtagatt ttcccgcacc
     1561 attagggccg agaatggcaa caatttcccc accgggaaaa gtcagcgaga cattatcagt
     1621 cagacgccgc ccctgaaggc tataaaccag attctgggcg ctgatcatgt gatcctccga
     1681 tttcgcagaa tcaaccacat aaaataaggg ccaccaagca ggctggttaa taatcccacg
     1741 ggcatttctg ctggctgtac cagcgtgcgg gcaagcgtgt ctgccatcag caataaacag
     1801 gctccggcga gggcggaaca agggatgagc cagcggtgat ttgctccggt agtcatgcga
     1861 atcagatgcg ggatcaccag gccaataaag ccgataatgc cgcttaccga tacggcagca
     1921 ccaacgagca gtgagctcac taatagtaat tgctggcgtt tacgcttaac gttcacgccg
     1981 aggtaatggg cttcttcatc accgagctgc agtaaattca gcgttccggc gagacaagtt
     2041 gttgcgataa tggccggaag gatgaatgaa ctggcaacca ataacgtcgg ccattgcgcc
     2101 tgtccaagat tgcccatcat ccacaatgtg agttgtcgta gctgcggctc atcgccaata
     2161 tagctcagga tgccgatcgc tgcgccgcac agggcattga tggcgatacc ggcgagtaat
     2221 aattgcatca tgctgccatc gcgatggcgc tgcgtgatga gaaaaatgat ggcgcagact
     2281 actaaacttc cggcaatggc aaacaccatt tgctcataga gcaccagcac cactgggaag
     2341 gagaagggca gcacaattgc aacgccaacc attaatgcag aaccgctact gacgccgagt
     2401 aatcctgggt cagccattgg gttgcgaaaa agcccctgca taattacccc tgcggttgcc
     2461 aacgctgcac ctaccagcac cgccagcaaa acccgtggta gacgaatatt gagccagata
     2521 tcgcggtatt cctcatcaaa aagggcatca aaactgatct ttaacgctcc ctgactagca
     2581 ccgaacagca ccagggcaag caataatagt gatagcccca taaagaccct ggcggatgaa
     2641 aatgaatcct tgagcacgca aaactccgtt acgacattac ggcgcggaca tgccgcgccg
     2701 gggtatcacg ccggtttcag gtccggttca atcagactgt agacctgctg gttaagtgct
     2761 ggcgcatttg ccagttcatg gatatgtgcg gcgacatcgg cacggcggac aaaaccatga
     2821 cactcctgat tttgtatccg ttgtgctttg cctgttgctg cgccgtcaag cagaccacca
     2881 gggcggagaa tggcgtagtc tagctggctg gtttgcagcc agctttccgc cagcgttttt
     2941 tcccgtaccg cctgaccaaa cgcagccttt gcccgctccg ataaaaacgg ccagctatcg
     3001 ccgcatccca aagaggtcac cagaatcatg cgactgatgc cggccttttc ggcctcatca
     3061 atcactgttc tgtgcgccag ataatcctga gctcctccca ttgttgagat gataagcgca
     3121 tccgggccag ctgcgcggca agcagccgcg actacgctgg catcacaggc gtcgcctgta
     3181 aaaacctgta cgccttgttg cgccagtttc gtggcggcat cggcatgacg aatgacagca
     3241 acgaccggac gttgttccgc cagcgccagt tcaagcgttc tggcacccac accttttccg
     3301 cctgcgccaa agagtagcca cggcgtcatc aggcgtgttc ctttaacgat gccgccagag
     3361 tatggaaggc gctgacttgt tcgctcagga gctgacgatg gtcatccctg ccgaggaaga
     3421 ttttcagcat ggcgctgcct tctttgttga aaaagagaat ggacgcggtg tccatgccca
     3481 taaatttacg ttcgattaag gcaatatgcg tgcagttttc tgctttgata tgcccggaca
     3541 tgccgtgctt tccgcgtagg ttgaaatagc cgtggcgatg gaaacccgaa ggcagttcgc
     3601 cgctaaattc gaggattaca tcggcagtat gtactaacgt ggtgacgttg ccccattcgc
     3661 agacggtatc ccagacagta tcgaatttat cgcctggcac tacggtagag gagggtagat
     3721 ttctcactac ctccagcagt gtggtgttgt attgttcggc taccacctcc agcgtaccgt
     3781 ccggctccgt tttcaaaaat tcttgtaacg agacatggct cataaatcac tcctgttatg
     3841 gtttttcacg cataatcgct ggcgcattaa gtacctgaat tagttcatta agagactgca
     3901 aaatattgct tgcccagaaa cgaccttcat tagtcagccg caggcaagtt gaagcatcgc
     3961 gacttaagcc tttttggtgc cattgagcca gtaacggcgc gagtttttcg gcatgtggcg
     4021 taagttcatc cagcggtacg cgcgctgttt caacacctga ctgcaaggta tgacgccatt
     4081 gcgcattacg ttcggcgtta cgcatgatca acatcagcgg ttttttgcct gccgcgacgg
     4141 attcatgcca ggtctgaaga ttgcgttcgt tcatccagga gtaaccatta atcgaaccgc
     4201 cggctccgga accaaaggcc agacaatcgg caccttgttt aatcagcagg ttatagagat
     4261 tgcgttcgcg tgtggtacgg ccccagtggc tgttactgat gcagcgccaa ccggcatcgt
     4321 ccataaaatc acatccttgc agataaagat cgcgacgttc tgccggtgag ggtacggtag
     4381 tacgcccatt ttccacggcc ttgcccagcg gtgtattgga caggacattg agcgcataga
     4441 gatcgacgcc gtcgagaccg atatcgcggg caatagccag atcttctccc caggtttgcg
     4501 catcctgacc cggcaggcca aacagcaggt cacagaccac cgcagcacgg tcacgtttga
     4561 ccaggctttc cataaacgca atagcggttg ggccatccga ggtgcgggcc attttcttgc
     4621 ggattttgct gttaaacgac tggataccaa ttgagaagcg gtttgctcct gcatcaaggc
     4681 aagcatcgat tcgctcagcg tcaaaattca atacccgtcc ttcgatagtg atttcacaat
     4741 caggggccag tggcagcttt tctcgtaatg tggtgatgat tctggccaaa tcgtgtgccg
     4801 aaagtgcgga aggcgtaccg ccaccgaaat agaccgcgtg gatgggggca gactgatgca
     4861 atacgctgtc cgcttccatt tctatttcac gaatcagggc gtcggtataa tgagcacacg
     4921 catcttcatt aaaacggttc tgataaaaac cacaaaacgt gcagtgtgtc gcacaaaaag
     4981 gaatgtgtag gtaaaccagt cgcttacgag gtgatgccgt ttgattgatc atctcttgcc
     5041 aggtctgtgc cagttgctct ttggcaaccg gaatggctcc acgaaacggc atcatggcgc
     5101 gcctgtcttt aaatggctga tcaccgtcta aagcaaaatg tggcgtgaga tccagggtgt
     5161 tatttgcgtt cattcgtctg atttaaatcc atttattgcg gccaaagctg ctggtgtaaa
     5221 gactcaacga catcagcaat acgcggaccc attcccagaa tcaggttttg atcaacggtg
     5281 ataatgcgct ggtttttcca tgcggcagtg tgggtaattc cagcaataga acgtagccga
     5341 ttaatatcgc catcgaccat ttgtgaggtt acgacaatca cttctggatt agccgcaatc
     5401 agtgattctg cactgtagct tttgtactgc tggtgggtag cgacgttttc tgctccggca
     5461 agcgaaagaa tggcgtccgc gacgctgcct ttaccggcaa cttgtggcgc acttccgcct
     5521 gcggagagaa taaacatggc tttaaccggg gcttttttgg tcgccacgtt ttgctgtact
     5581 cgctccaggc gttggctgat ttgtgtcacc agcgcctcgc cttgttcagg aacctgtagc
     5641 gtttttgcca gttggcgaat gttggcgtac atctgctgaa gggtggcagg cacacgcggc
     5701 agggtgacga cgttgacttt ctgcgctcgt agttggtcga gcacaatttg tggcccggca
     5761 tcctgccagg taattacgct atccgggcga agtgacaaaa tgccttcgct gctgagttgt
     5821 ttccagtagc caatatgcgg cagtttggcg gtttctggtg gataagatgt cgtttcatcg
     5881 acaccaacca cgcgttcgcc agcgcccatc gcgtagatta gctccgtcag cgatcctcct
     5941 gcgaccacga tacgttcggc agccgttacg ctaaaagccc agctaagagc cagtactgcc
     6001 gtaaacaatt ttttacgttt tactgcggtc ttcgcggcag aggccacggc tgaaatacat
     6061 aacgtcagtg gggaaaaaag aaacggcctg gtgatgatcc ttggcataat atgagaactc
     6121 ttatcaaatt gagaatgatc atgattatca tttgagttat atatgaaata caaccgtcga
     6181 ttcaatacgc aaggcgttac aagcgtattt atttttgatt tttatcaaga tgcttcgtca
     6241 gctaacgttc ttgtttccga ctaaatttca tcgcgggaaa aagctgagaa cccagagata
     6301 tcgaggcttg cagagcaaag agaagaaaaa attatcactg ttaacgacaa aatttagtga
     6361 tgaggcatgg ggggataacc ataaaaacaa ggtggtttac agggcgcagg ataggtttca
     6421 taaccccctg tttattgtgt taatggtggt taaaaaaaaa cttgatcata aaaaattgat
     6481 cgataataat tctcattatc acgtttgcat ttaacaaaac acgaccatat ggataacaaa
     6541 accatagcat ttgcaaggga taacgcacag ctctcttcac tcctgatggg gcatatcgtt
     6601 ttgcctgaca aagagggcgt gcttttgcgt aattgccgag ttatcaggca atttcatggg
     6661 atataaacgc ttgtaaactc acccggcgaa tattatcgct ctgaatttta aataaattta
     6721 tttttcgcca gccaccccgg tattaaccca ggcagccagc attttttatt ttcctcatgg
     6781 agaatcgcta tgtcacgtcc gcaatttacc tcgttgcgtt tgagtttatt ggccttagct
     6841 gtttctgcca ccttgccaac gtttgctttt gctactgaaa ccatgaccgt tacggcaacg
     6901 gggaatgccc gtagttcctt cgaagcgcct atgatggtca gcgtcatcga cacttccgct
     6961 cctgaaaatc aaacggctac ttcagccacc gatctgctgc gtcatgttcc tggaattact
     7021 ctggatggta ccggacgaac caacggtcag gatgtaaata tgcgtggcta tgatcatcgc
     7081 ggcgtgctgg ttcttgtcga tggtgttcgt cagggaacgg ataccggaca cctgaatggc
     7141 acttttctcg atccggcgct gatcaagcgt gttgagattg ttcgtggacc ttcagcatta
     7201 ctgtatggca gtggcgcgct gggtggagtg atctcctacg atacggtcga tgcaaaagat
     7261 ttattgcagg aaggacaaag cagtggtttt cgtgtctttg gtactggcgg cacgggggac
     7321 catagcctgg gattaggcgc gagcgcgttt gggcgaactg aaaatctgga tggtattgtg
     7381 gcctggtcca gtcgcgatcg gggtgattta cgccagagca atggtgaaac cgcgccgaat
     7441 gacgagtcca ttaataacat gctggcgaaa gggacctggc aaattgattc agcccagtct
     7501 ctgagcggtt tagtgcgtta ctacaacaac gacgcgcgtg aaccaaaaaa tccgcagacc
     7561 gttgaggctt ctgaaagcag caacccgatg gttgatcgtt caacaattca acgcgatgcg
     7621 cagctttctt ataaactcgc cccgcagggc aacgactggt taaatgcaga tgcaaaaatt
     7681 tattggtcgg aagtccgtat taatgcgcaa aacacgggga gttccggcga gtatcgtgaa
     7741 cagataacaa aaggagccag gctggagaac cgttccactc tctttgccga cagtttcgct
     7801 tctcacttac tgacatatgg cggtgagtat tatcgtcagg aacaacatcc gggcggcgcg
     7861 acgacgggct tcccgcaagc aaaaatcgat tttagctccg gctggctaca ggatgagatc
     7921 accttacgcg atctgccgat taccctgctt ggcggaaccc gctatgacag ttatcgcggt
     7981 agcagtgacg gttacaaaga tgttgatgcc gacaaatggt catctcgtgc ggggatgact
     8041 atcaatccga ctaactggct gatgttattt ggctcatatg cccaggcatt ccgcgccccg
     8101 acgatgggcg aaatgtataa cgattctaag cacttctcga ttggtcgctt ctataccaac
     8161 tattgggtgc caaacccgaa cttacgtccg gaaactaacg aaactcagga gtacggtttt
     8221 gggctgcgtt ttgatgacct gatgttgtcc aatgatgctc tggaatttaa agccagctac
     8281 tttgatacca aagcgaagga ttacatctcc acgaccgtcg atttcgcggc ggcgacgact
     8341 atgtcgtata acgtcccgaa cgccaaaatc tggggctggg atgtgatgac gaaatatacc
     8401 actgatctgt ttagccttga tgtggcctat aaccgtaccc gcggcaaaga caccgatacc
     8461 ggcgaataca tctccagcat taacccggat actgttacca gcactctgaa tattccgatc
     8521 gctcacagtg gcttctctgt tgggtgggtt ggtacgtttg ccgatcgctc aacacatatc
     8581 agcagcagtt acagcaaaca accaggctat ggcgtgaatg atttctacgt cagttatcaa
     8641 ggacaacagg cgctcaaagg tatgaccact actttggtgt tgggtaacgc tttcgacaaa
     8701 gagtactggt cgccgcaagg catcccacag gatggtcgta acggaaaaat tttcgtgagt
     8761 tatcaatggt aatcatctgc cccgatattt cggggcattt atctggaagg aagagagaca
     8821 atgaaccact acacacgctg gcttgagtta aaagaacaaa atcccggaaa gtacgcgcgt
     8881 gacatcgcag ggttaatgaa tattagagaa gcagaactgg catttgcacg cgtcacgcac
     8941 gatgcgtggc ggatgcacgg cgatatccgt gaaattctgg cggcgctcga aagtgttggc
     9001 gaaaccaaat gtatttgtcg taatgaatat gcagtccatg agcaagttgg tacgttcaca
     9061 aaccagcatt tgaacggaca tgccggattg atcctcaatc cgcgcgcgct ggatttacgt
     9121 ctgtttctca atcaatgggc cagtgttttc cacatcaaag aaaacacggc tcgtggcgaa
     9181 cgccagagta ttcagttctt tgatcatcag ggcgatgcat tactaaaagt ttatgccacc
     9241 gacaataccg atatggcggc atggagtgag cttctggcac ggtttatcac cgatgagaat
     9301 acgccgcttg agttaaaagc cgttgatgcg ccagttgttc aaacgcgagc cgatgccact
     9361 gtggtcgagc aagagtggcg ggcgatgacc gacgttcatc agttttttac gttgctcaag
     9421 cgccacaacc tgacgcgcca acaggcgttc aatctggtgg cagacgattt ggcctgcaaa
     9481 gtatccaaca gtgcgttggc gcaaattctt gaatctgcac agcaggatgg taatgaaatc
     9541 atggtgtttg ttggcaaccg tggctgcgta cagattttca ccggtgtggt agaaaaagtg
     9601 gtgccaatga aaggttggct gaatattttc aacccgacgt ttactcttca tctattagaa
     9661 gagagcattg ctgaagcctg ggttacccgt aaaccgacca gcgatggcta cgtaaccagt
     9721 ctggaattgt ttgcccatga tggtacgcag atagcgcaac tttatggtca acgtacagaa
     9781 ggcgaacagg agcaagcgca atggcgtaag caaattgctt cgctgatacc ggaaggcgtt
     9841 gctgcataac gtataattaa cgggaggtct ggctatttat tgctgggcct ctttatattg
     9901 atcaatcgtt cacaccagat aatcaatatg ctgatggcgt accagctcat tgatccgctt
     9961 aagattgagg atcatcatga tattgtgttt atggcagtag aacgttttta gcgatatttt
    10021 cagtttctcc agaatatctt ccgttgtcat ttgattcgca atgcagctca gaacctgatg
    10081 ttgcttgcgc gaaagtgaca cattcgcttc aggcacagag cagtaactgc cattgatgag
    10141 tgcgacaaaa tcaatgatag tgatagtccg cggaacaaag gtcaccggca ctttccccag
    10201 gcagcgttta atattaaagg gggagaaaat aatgacatgg acaggtttta atcgttcaat
    10261 ttgagtgaga aaattagaat gaaatacatt attcattaat gtatcaataa tgacgaaggc
    10321 attctgatgc aacattacgt cgatctcttc ttcgttctgt atatgaacga cattaccttt
    10381 actcagaatg tttttcatcg cggtgaagaa catcgtatcc ctggtaatta taagaaacat
    10441 aaccgactcc tggtgcggac attaacttta taatcagttt gccaaaggta attaacagga
    10501 cagggcttat aataataata gttagcttac tgtgtattaa tttgatgtaa tgcatgatgc
    10561 agatgctgag aaagcatctg catctttcgg tggtgttatt tgaccagctc aggtgttacc
    10621 tgactcaccg cattggtgta gtaaggcgca ccccagcccg gttccggcca gaatgcacca
    10681 tagccgtaat cccacaggtt ataggtggta ttaaccactt ctctcaaatg ccacacctgg
    10741 atgccctgca tattcacttc caggaagtta tacgggactt tattgataaa gccaggttgt
    10801 tcaccctgaa tggtgccgag gatggtaaca aagtgattac gatagttcac tggatcaagg
    10861 aagccgcttt gtctggcgag cagtcggccc tgatagttgg cttcaatatc aggcttcgca
    10921 tagctatcca acggtaatac agcgatttct aacaacgtat ccgttttgcc attgataacg
    10981 ttgataacct tcccaccaaa gcgcgcttgt tgaccaacat ataaccccgg ctggttatga
    11041 acagcaacaa aacttttttg aatatcaggt tggttatttc ctttgatatt ttgcggaatt
    11101 gaactacatg ctgcaagcaa aaatgaaagg ctgagtatga gtgcaccttt tgtcatgttc
    11161 atgttactat ccttatcaac aaatattaaa accataatag atatttcttt atagttttca
    11221 tctgattctg aggcgatatg cctgatagta acgtaaacct gtctgatgtt tatatccctt
    11281 ctgtttagtt caattccgtt attttggggc gtggcatgag
//
LOCUS       DBFIFI010000138        11295 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.138, whole genome shotgun sequence.
ACCESSION   DBFIFI010000138 DBFIFI010000000
VERSION     DBFIFI010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11295
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.138"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(20..559)
                     /locus_tag="N7B39_003419"
     CDS             complement(20..559)
                     /locus_tag="N7B39_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2423340.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGSVTFNGKVIAPACT
                     LVAATKDSVVTLPDVSATKLQTNGQVSGVQTDVPIELKDCDTTVTKNATFTFNGTADT
                     TQITAFANQASSDAATNVALQMYMNDGTTAIKPDTETGNILLQAGDQTLTFKVDYIAT
                     GKATSGNVNAVTNFHINYY"
     gene            915..1490
                     /gene="ssuE"
                     /locus_tag="N7B39_003420"
     CDS             915..1490
                     /gene="ssuE"
                     /locus_tag="N7B39_003420"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HGU2423341.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAA
                     EDLLYARFDSPALHTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLHSLRGNAHA"
     gene            1483..2442
                     /gene="ssuA"
                     /locus_tag="N7B39_003421"
     CDS             1483..2442
                     /gene="ssuA"
                     /locus_tag="N7B39_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HGU2423342.1"
                     /translation="MRNIIKLALVGLLSVSTIVVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLLYVG
                     VEPPKPKAEVILVAENSPIKTAADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            2439..3584
                     /gene="ssuD"
                     /locus_tag="N7B39_003422"
     CDS             2439..3584
                     /gene="ssuD"
                     /locus_tag="N7B39_003422"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HGU2423343.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVDFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVENDFIPRRVAQS"
     gene            3596..4387
                     /gene="ssuC"
                     /locus_tag="N7B39_003423"
     CDS             3596..4387
                     /gene="ssuC"
                     /locus_tag="N7B39_003423"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HGU2423344.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVTVWQLASSVGWLSTRILPSPEG
                     VVMAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            4384..5151
                     /gene="ssuB"
                     /locus_tag="N7B39_003424"
     CDS             4384..5151
                     /gene="ssuB"
                     /locus_tag="N7B39_003424"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HGU2423345.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQEHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMQRGESETRLRKQG"
     gene            complement(5194..7806)
                     /gene="pepN"
                     /locus_tag="N7B39_003425"
     CDS             complement(5194..7806)
                     /gene="pepN"
                     /locus_tag="N7B39_003425"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HGU2423346.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLCLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            8072..9274
                     /gene="pncB"
                     /locus_tag="N7B39_003426"
     CDS             8072..9274
                     /gene="pncB"
                     /locus_tag="N7B39_003426"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HGU2423347.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQVQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLEIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9443..10843
                     /gene="asnS"
                     /locus_tag="N7B39_003427"
     CDS             9443..10843
                     /gene="asnS"
                     /locus_tag="N7B39_003427"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HGU2423348.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQTSKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTEAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2628 a         2830 c         2941 g         2896 t
ORIGIN      
        1 aaacaattaa tgagttctat taataatagt taatatggaa atttgttacc gcattcacat
       61 taccagaagt cgctttcccc gtagcgatat aatcaacttt aaaagtcaac gtctgatctc
      121 cagcttgcaa caaaatgttc ccggtttctg tgtctggctt gatggccgtt gtaccatcat
      181 tcatatacat ttgcagggcc acgtttgtag cagcatcaga tgaggcctgg ttagcaaacg
      241 ctgtaatctg agtagtatcc gcagtgccat taaaggtgaa cgttgcattt tttgttacgg
      301 tagtatcaca atcttttaat tcaattggca catcagtttg cacgccagaa acctgaccat
      361 tggtttgcaa cttcgtggca ctaacatctg gcaaagtcac cacggaatct ttcgtcgcag
      421 ctaccagggt acaagctgga gcaataactt taccattaaa tgttactgag ccatccgtga
      481 tagcattatc atcagcagcc ataacgctgg acgttgcaca taccacagtt ataaacgccg
      541 tcaatacact ttttttcatc gttatcatcc tgatctcttc gtaaatatga taatgccttt
      601 aaatgacatt agacttaata ataacaataa ttgtccgcca tttaaatcaa gcatactaaa
      661 tccaataagt gacaaatctt taatttatag atttcatgaa tatttaaata ttacctgaac
      721 gagtaattca ttgattcaac atctaatgac tatccaataa ataaatatgc gtgatatata
      781 aaaccctttc tttagtttat tttcagaaaa agatacacaa aatttatatt tggcattttc
      841 ttgtctctcc gacagactga gcgaattgtt atcaatgtta acaaaacaag aacaattggt
      901 tataaggaga gagtatgcgt gtcatcaccc tggcgggtag tcctcgcttt ccttctcgct
      961 ccagctcctt gctggaatat gcgcgggaaa aactaaatgg cctggatgtt gaggtttatc
     1021 actggaatct gcaaaacttc gccgcggaag atctgcttta tgctcgtttc gatagtccgg
     1081 cactccatac cttcaccgaa cagttgcaac aggctgacgg gctgattgtc gccacgcctg
     1141 tgtataaagc cgcttattcc ggtgcgctga aaaccctgct cgacctgctg ccagaacgtg
     1201 cgttgcaagg caaagtggtg ctaccgctgg cgacgggcgg tacggtggcc catctgctgg
     1261 cggtcgatta tgcccttaag ccagttttaa gtgcgctgaa agcccaggag atcctgcacg
     1321 gcgtgtttgc cgatgactca caagtaattg attaccatca caaaccccag ttcacgccga
     1381 atttacaaac ccgtcttgat accgcgctag aaactttctg gcaggcattg caccgccgcg
     1441 atgttcaggt tcctgatctt cattctctgc gaggtaatgc ccatgcgtaa catcattaaa
     1501 ctggcgctgg tgggattgct tagcgtctct acgattgtgg ttgctgcaga atcctcgcct
     1561 gaagcgttac gtataggcta tcagaaaggc agtattggta tggtattggc aaaaagccac
     1621 cagttactgg aaaaacgcta tccgcaatca aaaatctcgt gggtggagtt ccccgctggc
     1681 ccgcaaatgc ttgaggcgtt aaacgttggc agtattgatc tcggcagtac cggggatatt
     1741 ccgccaatct tcgcccaggc tgccggggct gatttgctgt acgtgggcgt cgagccgccg
     1801 aagccaaaag ccgaggtgat tctggtggca gaaaacagcc cgatcaaaac cgcagccgat
     1861 cttaaaggcc acaaagttgc gttccagaaa ggttccagtt cacacaatct tttactgcgc
     1921 gcactacgcc aggccggact taagtttact gacatccagc ccacttatct gacgccagct
     1981 gatgcccgcg ccgcgttcca gcaaggtaac gttgacgcct gggctatctg ggatccctac
     2041 tactccgctg cattattaca gggcggcgtg cgggtgttga aagacggcac cgatctcaat
     2101 caaaccggat cgttttatct ggcagctcgc ccctatgcag aaaaaaacgg cgcttttatt
     2161 cagggtgtac tggcaacctt tagtgaggcc gatgcgttaa cccgcagcca gcgcgagcaa
     2221 agtatcgctt tactggcaaa aacgatgggc ttaccggcac cggtgattgc ctcttactta
     2281 gatcatcgcc ctcctaccac catcaaaccg gttaacgccg aggttgccgc cttacagcag
     2341 caaacggcag atctgtttta tgaaaatcgt ctggtgccga aaaaagtcga tattcgccaa
     2401 cgcatctggc agcccactca actggaagga aaacaattat gagtctgaat atgttctggt
     2461 ttttaccgac ccatggtgac gggcattatc tgggaacgga agaaggttca cgcccggttg
     2521 atcacggtta tctgcaacaa attgcgcaag cggcggatcg tcttggctat accggtgtgc
     2581 tgatcccaac ggggcgctcc tgcgaagatg cgtggctggt tgccgcatcg atgatcccgg
     2641 tgacgcagcg gctgaagttt cttgtcgccc tgcgtcccag cgtaacctca cctaccgttg
     2701 ccgcccgcca ggccgccacg cttgaccgtc tctcaaatgg acgtgcgttg tttaacctgg
     2761 tcacaggcag cgatccacaa gaactggcag gcgacggcgt gttccttgat catagtgagc
     2821 gctacgaagc ctcggcggaa tttacccagg tctggcggcg tttattgctg ggggaaaccg
     2881 tcgatttcaa cggtaaacat attcatgtgc ggggagccaa actgctcttc ccgccgattc
     2941 aacagccgta tcctccgctt tactttggcg gatcgtcaga cgtcgcccag gagctggcgg
     3001 cagaacaggt tgatctctac ctcacctggg gcgaaccgcc ggaactggtt aaagagaaaa
     3061 tcgaacaagt gcgggcgaaa gctgccgcgc atggacgcaa aattcgtttc ggcattcgtc
     3121 tgcatgtgat tgttcgtgaa actaacgacg aagcgtggca ggccgccgag cggttaatct
     3181 cgcatcttga tgatgagact atcgccaaag cacaagccgc attcgcccgc acggattccg
     3241 tggggcaaca gcgaatggca gcgctacata acggcaagcg cgacaatctg gagatcagcc
     3301 ccaatttatg ggcgggcgtt ggcttagtgc gcggcggtgc cgggacggcg ctggtgggcg
     3361 atggtcctac ggtcgctgcg cgaatcaacg aatatgccgc gcttggcatc gacagctttg
     3421 tgctttcggg ctatccgcat ctggaagaag cgtatcgggt cggcgagttg ctgttcccac
     3481 atctggatgt cgccattccg gaaattcccc agccgcagcc gctgaatccg caaggcgaag
     3541 cggtggagaa tgattttatc cctcgcagag tcgcgcaaag ctaaggaaga agataatggc
     3601 aacgccagtg aagaagtggt tattgcgcgt tgccccctgg tttttaccgg tgggcatcgt
     3661 gacggtgtgg caactggcct cctcggttgg ctggttgtcg acgcgtattt tgccctcacc
     3721 ggaaggggtg gtgatggcgt tctggacgct ctcagccagc ggcgaattgt ggcagcatct
     3781 ggcgatcagc tcctggcggg cgctgattgg tttttcaatt ggcggctcgc tggggttgat
     3841 tttgggactg attagcggac tgtcgcgctg gggagaaagg ctgctggata cctcaattca
     3901 gatgttgcgc aacgtgccgc atctggcgct gatcccgctg gtgattttgt ggtttggcat
     3961 tgatgaatcc gcaaaaatct ttctggtggc gctcggtacg ctattcccca tttatatcaa
     4021 cacctggcat ggcatccgta atatcgatcg cgggttggtg gagatggcgc gtagctatgg
     4081 attatccggc ataccgctgt ttatccatgt gatcctgcct ggtgccctgc cctcaattat
     4141 ggtcggcgtg cgttttgcgt tgggcctgat gtggctgacg ctgattgttg ccgaaaccat
     4201 ttctgccaat tcaggcattg gttatctggc gatgaatgcg cgggagtttt tgcaaacgga
     4261 cgtggtggta gtcgctatta ttctttacgc cctgctcggc aaacttgctg acgtcagcgc
     4321 gcagctgtta gagcgcctct ggttgcgctg gaacccggct tatcatttga aggaggccac
     4381 ggtatgaata ctgctcgtct gaaccagggc acaccattgt tgctcaatgc agtaagcaaa
     4441 cattacgcgg aaaatatcgt cctgaaccaa ctggatttac atattccggc aggtcagttt
     4501 gtggcggtgg tgggccgcag cggtggtggc aaaagtaccc tgctgcgcct gctggcaggt
     4561 ctggaaacgc caaccgctgg cgatgtgtta gcgggcacca caccgctggc tgaaattcag
     4621 gaagacacgc gaatgatgtt tcaggatgcg cgtctgctac cgtggaaatc ggtgattgat
     4681 aacgttgggt taggccttaa aggccagtgg cgcgatgccg cgcgtcgggc gttggctgcg
     4741 gtaggactgg agaatcgcgc cggggaatgg cctgccgcac tttctggcgg gcagaaacag
     4801 cgagtggcgc tggcaagagc gttgattcat cgaccgggtt tattgttgct tgatgaaccg
     4861 ctcggtgcgc tggatgcctt aacgcgactc gagatgcagg atttgattgt gtcactttgg
     4921 caggagcacg gttttaccgt actgttggtg acgcatgatg tgagcgaagc ggtagcaatg
     4981 gctgatcggg tgttgttaat tgaagaggga aaaattggtc tggatttgac ggtggatatt
     5041 ccccgtccgc gccggttagg gtcggtgcga ttagcggagc tggaagcgga agtgttgcaa
     5101 cgggtgatgc agcgtggtga atctgaaact cgcctgagaa aacaggggta aaatccccga
     5161 atggcggcgc tattgccgcc attcggggat ttattaagcc agtgctttgg tgatcttctc
     5221 gtacagatcg ccagatagat tttccagccc tttcaactgt tccagcgccg cgcgcatttt
     5281 ctcctgacgt ttggcatcat aacgtttcag gcgaatcagc ggttcaatca ggcgtgaagc
     5341 tacctgcggg ttacggctgt tcaggtcggt aagcatttcc accaggaact ggtaaccgct
     5401 cccatcttcg gcatggaacg ctgccgggtt gctgcccgca aacgcaccaa tcaacgaacg
     5461 aatgcggttc gggttgctca tggtaaatga gcgatgctgc aacaggccgc gcaccgtctc
     5521 cagcacattc gccgccgggc tggtggcttg caggataaac catttatcca tcaccagacc
     5581 atcctgatgc cacttgtcgt cgtactcctg catcagcgcg tcacggcaag gcagctgtgc
     5641 agcaaccgcc gcagaaagcg ctgccagcgc atcggtcata ttgtttgctt catggtattg
     5701 cttgctcacc agcacgtcag ccagatgcgt ttcaccaaaa gcaaggaagc gcaggcaggc
     5761 attacgcaga gtgcgttttg caatatcttc atgctcaaca cggtactcgc tctggtaatt
     5821 cgcgttgtaa atagccagta gctcatccgc cagttcagtc gccagagtac gagtgagtgc
     5881 ttcgcgtact tcggcaatag caatcgggtc gatgatatcg aacagttcag ccatttcatt
     5941 gacagaaggc agcgtcagga tttctgccgc cagcgccgga tcaatcttct catcgagcag
     6001 caccgcgcgg aaagcgtcag ccacatgtac cggcagagac agcggctgcc cttgctgatg
     6061 acgggcgacg ttcagcttga tgtaggttgc cagcaaactt tgcgccgcat cccaacggga
     6121 aaaatcatta cgcgcatgac gcatcaggaa ggtcagttgc tgatcgctcc acttatattc
     6181 cagtttcact ggcgcagaga attcgcacag cagcgcaggc accggctgga agtagacatt
     6241 atcaaaaaca aaggtctgtt ccgcctgggt gacgttcagc acggaattca ccggatgacc
     6301 gcctttctgc aacgggatca ctttgccttc gttgtcatac aattcgatgg caaacggaat
     6361 atgcagcggc tgtttttctg cttgatccgg cgtggctggc gtgcgctggc tgatggtcag
     6421 ggtgtactgc tcggtttccg gattgtagtc gtctttgacg gtcacaaccg gcgtaccgga
     6481 ctggctgtac caacggcgga aatgggagag atcgacattc gacgcatctt ccatcgcctg
     6541 aacgaaatcg tcgcaggtcg ctgcactgcc atcatggcgc tcgaaataga gctgcatccc
     6601 tttctggaag ttttcttcgc caagcagagt gtggatcata cgaatcactt ccgcgccctt
     6661 ctcgtaaacg gtcagggtgt agaaattgtt catctcaatg accatgtccg ggcggattgg
     6721 gtgcgccatc gggctggcgt cttccgcaaa ctgcaatccg cgcatggtgc gcacattatt
     6781 gatacggttt accgcgcggg aaccaaggtc agagctgaat tcctgatcgc ggaagacggt
     6841 taaaccttct ttcaggctga gctggaacca gtcgcggcag gtcactcggt taccggtcca
     6901 gttatggaaa tattcatggc cgataacgcg ttcaatatcg aggtaatctt tgtcggtggc
     6961 ggtatcggtg cgggccagca catatttgga gttaaagata ttcagaccct tattctccat
     7021 tgcgcccata ttgaagaaat ccaccgcgac gatcatatag atgtcgaggt catactccag
     7081 gccaaagcgt tcttcatccc atttcatgga gtttttcagc gaggtcatcg cccacggcgc
     7141 gcgatcaagg ttgccgcgat cgacgtacag ctccagtgct acttcgcgac cagaacgcgt
     7201 ggtaaaggta tcgcgcagta catcaaagtc gcctgccacc agcgcaaaca ggtagcacgg
     7261 tttcgggaac gggtcctgcc actgtaccca atggcgtccg ttttccagtt cgccttgcgc
     7321 aacgcggtta ccgttggaaa gcaggaaggg atatttggtt ttatcggcaa taattttggt
     7381 ggtaaaacgc gccagcacgt ccgggcggtc gagataatac gtaatatggc ggaaaccttc
     7441 ggcttcacac tgggtgcaaa gcgcatcgcc tgactgataa agcccttcca gcgccgtatt
     7501 cgccgccggg cttatttcat taatgatctt aagcgtaaaa cgctccggca aattactgat
     7561 gaccagtgcg ccctcttctt ctttccaggc ggtccacggc tcatcattaa tatgaacaga
     7621 aaccagtttg aggtcttcgc cgttgagaca aagcggagca tctgatgcac catgacggac
     7681 agcctggctg accgcggtaa cgaccgtctt ttgcgcgtcg aggtcaaagg tcaagtcaat
     7741 atcagtaatc tggtaatccg gcgcacgata atcgtgacgg tatttggctt gtggctgttg
     7801 agtcataaat aacctttagc atcttttata gagtctggtg ttcagtctat tcctgttgcg
     7861 taaatcgcgc tatgcagaat cttcatcttt tcaggtacaa acgcctttat tgctacattt
     7921 ttataacata caccgcgtaa tgccatcgac cagaaaggtg gcatatggtg tgatcggggt
     7981 tcaataaatt gcgaaacaag gtatactcca gcagttcctg aagatgttta ttgtactaaa
     8041 cgctcctgta cgaggacgct actgcgcacc tatgacacaa ttcgcttctc ctgttctgca
     8101 ctcgttgctg gatacagatg cttataagtt gcatatgcag caagccgtgt ttcatcatta
     8161 ttacgatgtg catgtcgcgg cggagtttcg ttgccgaggt gacgatctgc tgggtattta
     8221 tgccgatgct attcgtgaac aggttcaggc gatgcagcac ctgcgcctgc aggatgatga
     8281 atatcagtgg ctttctgccc tgcctttctt taaggccgac tatcttaact ggttacgcga
     8341 gttccgcttt aacccggaac aagtcaccgt gtccaacgat aatggcaagc tggatattcg
     8401 tttaagcggc ccgtggcgtg aagtcatcct ctgggaggtt cctttgctgg cggttatcag
     8461 tgaaatggta catcgctatc gctcaccgca ggccgacgtt gcgcaagccc tcgacacgct
     8521 ggaaagcaaa ttagtcgact tctcggcgtt aaccgctggt cttgatatgt cgcgcttcca
     8581 tctgatggat tttggcaccc gtcgccgttt ttctcgcgaa gtacaagaaa ccatcgttaa
     8641 gcgtctgcaa caggaatcct ggttcgtggg caccagcaac tacgatctgg cgcgtcggct
     8701 ttccctcacg ccgatgggaa cacaggcaca cgaatggttc caggcacatc agcaaatcag
     8761 cccggatcta gccaacagcc agcgagctgc acttgctgcc tggctggaag agtatcccga
     8821 ccaacttggc attgcattaa ccgactgcat cactatggat gctttcctgc gtgatttcgg
     8881 tgtcgagttc gctagtcggt atcagggcct gcgtcatgac tctggcgacc cggttgaatg
     8941 gggtgaaaaa gccattgcac attatgaaaa gctggaaatt gatccacaga gtaaaacgct
     9001 ggttttctct gacaatctgg atttacgcaa agcggttgag ctataccgcc acttctcttc
     9061 ccgcgtgcaa ttaagttttg gtattgggac tcgcctgacc tgcgatatcc cccaggtaaa
     9121 acccctgaat attgtgatta agttggtaga gtgtaacggt aaaccggtgg cgaaactttc
     9181 tgacagccct ggcaaaacta tctgccatga taaagcgttt gttcgggcgc tgcgcaaagc
     9241 gttcgacctt ccgcatatta aaaaagccag ttaatatcat cagggagcta atcggctccc
     9301 tttttttacc tttaattccg aaatctttcg ctgcatttgc gaattctgct tgtctgattg
     9361 cagatgccag gtaacatagg tatcccccca tttacgggtg gacaagtgtt tattttttcc
     9421 gactattaac agagagaata ttatgagcgt tgtgcctgta gccgacgtac tccagggccg
     9481 cgtagccgtt gacagcgaag tcaccgtacg cggatgggta cgtacccgcc gagattcaaa
     9541 agctggcatc tccttcctcg ccgtttatga cggttcctgc tttgatcctg tacaggctgt
     9601 catcaataat tctctgccca attacaatga agacgtcctg cgtctgacca ccggctgctc
     9661 ggtcattgtg acgggtaaag tcgtggcgtc gccgggccag gggcaacaat ttgaaattca
     9721 gaccagcaag gttgaagttg ctggttgggt tgaagatcca gacacttacc cgatggcggc
     9781 aaaacgccac agcattgagt atctgcgtga agtcgctcac ctgcgtccgc gcacaaacct
     9841 gattggtgcc gtcgcgcgcg ttcgccatac gctggcgcag gcgctgcatc gcttctttaa
     9901 cgagcaggga ttcttctggg tttcaacgcc actgattact gcgtctgata ccgaaggggc
     9961 tggcgaaatg ttccgcgttt ctacgctgga tctggaaaat ctgccgcgta acgatcaggg
    10021 caaagtggat ttcgacaaag acttctttgg taaagagtct ttcctgaccg tatctggcca
    10081 gttgaacggc gaaacctacg cttgcgcatt atccaaaatt tataccttcg gcccgacttt
    10141 ccgtgctgaa aactccaaca ccagccgtca cctggcggaa ttctggatgc tggagccgga
    10201 agtggcgttt gctaacctga acgatatcgc gggtctggct gaagccatgc tgaaatatgt
    10261 cttcaaagcg gttctcgaag aacgcgctga cgacatgaaa ttcttcgctg aacgcgtaga
    10321 taaagatgcc gtttcacgtc tggaacgctt tattgaggcc gatttcgcgc aggtggatta
    10381 caccgaagca gtaaccattc tcgaaaactg cggcaggaag tttgaaaacc cggtttactg
    10441 gggcgtcgat ctctcttctg agcatgagcg ttatctggcg gaagaacact ttaaagcacc
    10501 ggtagtggtt aaaaactatc cgaaagatat taaagcgttc tatatgcgcc tcaacgaaga
    10561 cggtaaaacc gttgcggcta tggatgttct ggctccgggc atcggtgaga tcattggtgg
    10621 ctcccagcgt gaagagcgtc tggacgtgct ggacgagcgt atgctggaaa tgggcctgaa
    10681 caaagaagat tactggtggt atcgcgatct gcgtcgctac ggtactgttc cgcattccgg
    10741 tttcggtctt ggttttgaac gcctgattgc ttacgtaact ggtgtgcaaa acgtgcgtga
    10801 tgtgattccg ttcccacgaa ctccgcgtaa cgccagcttc taatttagcg tctacaagag
    10861 ccagcacccg ctggctcttt tttatccttt ctgtcaagtt atctgtttgt taagtcaatc
    10921 aatctatttg caaccccgcc ataacgatca tcctgttacg gaatattaca ttgcaacatt
    10981 tacgcacaaa aaataacccg cattcttatt gcggattagt tttttcttag ctgatagcac
    11041 aattttcata ctattttttt ggcattctgg atgtctgaaa gaggattttg ggccaggtcg
    11101 ataaagtttc catcagaaac aaaatttccg tttagttaat ttaaatataa ggaaatcata
    11161 taaatagata aaaattgctg taaatatcat cacgcctcta tggaaatatg acggtgttca
    11221 caaagttcct taatttttac ttttagttac atattttttc tttttgaaac taaatcttta
    11281 tctttgtagc acttt
//
LOCUS       DBFIFI010000139        11202 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.139, whole genome shotgun sequence.
ACCESSION   DBFIFI010000139 DBFIFI010000000
VERSION     DBFIFI010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11202
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.139"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            111..245
                     /locus_tag="N7B39_003428"
     CDS             111..245
                     /locus_tag="N7B39_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HGU2423349.1"
                     /translation="MIYYLGYDRFLLIFFTDYSHFMDRITLPSTDTARGKGKWKIITA
                     "
     gene            224..328
                     /gene="speFL"
                     /locus_tag="N7B39_003429"
     CDS             224..328
                     /gene="speFL"
                     /locus_tag="N7B39_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HGU2423350.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            639..2837
                     /gene="speF"
                     /locus_tag="N7B39_003430"
     CDS             639..2837
                     /gene="speF"
                     /locus_tag="N7B39_003430"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HGU2423351.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVSDVE
                     RGKLDEIDATGYDIPVFIATENEERIPAEYLSRISGVFEHGEARKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEALFRADLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELITEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFIPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2834..4153
                     /gene="potE"
                     /locus_tag="N7B39_003431"
     CDS             2834..4153
                     /gene="potE"
                     /locus_tag="N7B39_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HGU2423352.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSHVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4210..5850)
                     /gene="pgm"
                     /locus_tag="N7B39_003432"
     CDS             complement(4210..5850)
                     /gene="pgm"
                     /locus_tag="N7B39_003432"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HGU2423353.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKAVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5876..6421)
                     /gene="seqA"
                     /locus_tag="N7B39_003433"
     CDS             complement(5876..6421)
                     /gene="seqA"
                     /locus_tag="N7B39_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HGU2423354.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPIKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6606..7370
                     /gene="ybfF"
                     /locus_tag="N7B39_003434"
     CDS             6606..7370
                     /gene="ybfF"
                     /locus_tag="N7B39_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HGU2423355.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIAHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFSAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7510..7803
                     /gene="ybfE"
                     /locus_tag="N7B39_003435"
     CDS             7510..7803
                     /gene="ybfE"
                     /locus_tag="N7B39_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HGU2423356.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7943..8473
                     /gene="fldA"
                     /locus_tag="N7B39_003436"
     CDS             7943..8473
                     /gene="fldA"
                     /locus_tag="N7B39_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HGU2423357.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8683..8769
                     /gene="uof"
                     /locus_tag="N7B39_003437"
     CDS             8683..8769
                     /gene="uof"
                     /locus_tag="N7B39_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HGU2423358.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8762..9208
                     /gene="fur"
                     /locus_tag="N7B39_003438"
     CDS             8762..9208
                     /gene="fur"
                     /locus_tag="N7B39_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HGU2423359.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9292..9618)
                     /gene="chiQ"
                     /locus_tag="N7B39_003439"
     CDS             complement(9292..9618)
                     /gene="chiQ"
                     /locus_tag="N7B39_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HGU2423360.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9668..11073)
                     /gene="chiP"
                     /locus_tag="N7B39_003440"
                     /pseudo
     CDS             complement(9668..11073)
                     /gene="chiP"
                     /locus_tag="N7B39_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
BASE COUNT         2731 a         2769 c         2782 g         2920 t
ORIGIN      
        1 gcgccaaata ttattatttg gcaggtttaa tttcttaacg caaatataaa acacgaaatt
       61 acaacattta agtaacaaaa cagcatctag tatggcgcta ttcataatta atgatttatt
      121 atctggggta tgatcgtttt ttgttgatct tcttcacaga ttatagccat ttcatggata
      181 gaataactct accttcaact gacacagcaa gaggtaaagg taaatggaaa ataataaccg
      241 cttaatgcct catataaggc ggacaaccca tatcatgaag tttgcccatc gtaatagttt
      301 tgactttcat ttctttaatg cccgctagtc ttctgactaa agggcacccc agcgtacagg
      361 tctccctgac tttaagcatt acaggttaat acctgtatcc ctcggtgctc atatactgct
      421 aaccctttta aactctacat aattcgagtc gcagcatatg caacttgaag tatgacgagt
      481 atagccagtt accgggctgg tctgggttat tgcatctgca aaaagcaaac tactgattta
      541 tttatcagcg gtggagcttt gctttttttc cggcgtgatc gatttctcct ttgagaaatt
      601 gaggacctgc tattacctga aataaagaga tgaacaaaat gtcagaatta aaaattgccg
      661 ttagtcgttc ttgcccggat tgtttttcca ctcatcgtgc atgcgtgaat atagacgaaa
      721 gtaattatat tgacgttgcc gccattattt tatcagtcag tgatgttgaa cgtggaaaac
      781 tcgatgaaat agacgctact ggctatgaca ttcctgtttt tattgcaacg gaaaatgaag
      841 aacgtatacc agcagaatat ctttcacgta tttctggtgt cttcgaacat ggagaagcac
      901 gcaaagaatt ttatggtcgt cagttagaaa ccgctgccag ccattatgaa actcaactgc
      961 gcccaccttt ctttcgcgca ctggtcgatt atgtcaatca gggcaacagc gcatttgact
     1021 gccccggtca tcagggcggc gaatttttcc gtcgccatcc ggcggggaat cagtttgtgg
     1081 aatactttgg tgaggcgctg ttccgtgccg acttgtgcaa cgccgacgta gcgatgggcg
     1141 atctgctgat tcacgaaggc gcgccatgca ttgcacagca acatgcagca aaagtgttta
     1201 atgccgataa aacctacttc gttttaaatg gcacttcatc ttctaacaaa gtggttttaa
     1261 acgccctgct gacaccgggt gatctggtgc tatttgaccg caataaccat aagtctaacc
     1321 accacggggc cttgctacag gctggtgcaa caccggttta tctggaaaca gcacgtaacc
     1381 cgtatggttt tatcggtggt attgatgcac actgcttcga agaaagttat ctgcgtgaac
     1441 tgattacaga agttgcaccg caacgggcaa aagaggcgcg tccgttccgc ctcgctgtga
     1501 ttcagctagg cacctacgac ggtacgattt ataacgcccg ccaggtggtg gataaaattg
     1561 gtcatctgtg tgactacatc ctgtttgatt ccgcatgggt cggctatgaa cagtttattc
     1621 cgatgatggc tgactgttcg ccgctgttgc tggatcttaa tgagaacgat ccgggtattc
     1681 tggttacgca atccgtacat aaacagcagg ctggtttttc tcaaacttca caaatccata
     1741 aaaaagacag ccacatcaaa gggcaacagc gttatgtacc gcacaaacgc atgaacaacg
     1801 cctttatgat gcatgcctcc accagcccgt tctatccgct gtttgccgca ctggatatca
     1861 acgccaaaat gcatgaaggt gtcagcggtc gtaatatgtg gatggattgt gtggtaaatg
     1921 gcattaatgc ccgcaaactg atcctcgata actgtcagca tattcgtccg ttcatacctg
     1981 aactggtgga tggtaaaccc tggcagtcgt atgaaacagc gcaaattgcg gttgatctgc
     2041 gcttcttcca gtttgtacca ggtgagcact ggcattcttt tgaaggctat gcagagaatc
     2101 aatactttgt tgatccgtgc aaactgttgc tgacaacccc aggtattgat gcacgtaacg
     2161 gcgaatatga agcgttcggt gtacccgcga cgattcttgc taacttcctg cgcgaaaatg
     2221 gcgtagtgcc ggaaaaatgc gatcttaact ccatcctctt cctgctgact ccggcagaag
     2281 atatggccaa acttcagcaa cttgttgccc tgctggtacg cttcgaaaaa ctgctggaat
     2341 ctgatgcgcc gctggcggaa gtgctacctt ccatctacaa acagcatgaa gagcgctacg
     2401 ccggttatac ccttcgtcag ttgtgccagg aaatgcatga tttgtatgcc cgccacaacg
     2461 tgaaacaact gcaaaaagag atgttccgta aggaacactt cccacgcgtc agcatgaatc
     2521 cgcaagaagc taactacgcc tatttacgcg gtgaagtgga gctggttcgt ctgccggatg
     2581 cagaaggccg tatcgctgcc gaaggtgcgc ttccttatcc tccgggtgtg ctgtgtgttg
     2641 ttccgggtga aatctggggt ggtgctgttt tgcgttactt cagcgctctg gaagaaggga
     2701 tcaacctgct gccaggtttt gcaccggagc tgcagggtgt ctatatcgaa gaacatgatg
     2761 gtcgtaagca agtttggtgc tatgtcatca agcctcgtga tgcgcaaagc accctgctga
     2821 aaggggaaaa attatgagtc aggctaaatc gaacaaaatg ggcgtcgttc agttaaccat
     2881 actgacgatg gtcaacatga tgggctccgg tatcatcatg ctgccgacaa agcttgccga
     2941 agttgggaca atctcaatta tctcctggct ggtgacagcc gtaggctcaa tggcactggc
     3001 atgggcattc gcaaaatgcg gtatgttcag ccgtaaatca ggcggtatgg gcggttatgc
     3061 cgagtatgct ttcggtaaat ccggtaactt tatggcgaac tatacctacg gcgtctcact
     3121 gctgattgct aacgtcgcga ttgctatttc ggcggttggt tacggcaccg aactgctcgg
     3181 cgcgagtttg tcgccagtgc agattggtct tgcgaccatc ggggtgctgt ggatttgtac
     3241 cgtggctaac tttggtggtg cgcgcattac cgggcaaatc agtagcatta ctgtgtgggg
     3301 agtcattatt ccggtcgtcg gtctgtgcat tattggctgg ttctggttta gcccgacgct
     3361 gtacgttgat tcctggaatc cgcatcatgc accgttcttc agtgcggtag gttcttccat
     3421 cgctatgacg ctgtgggctt ttcttggtct ggagtctgcg tgtgcaaata ctgatgtagt
     3481 ggaaaacccg gaacgtaatg tgccaatcgc ggtactcggc ggtacgttag gtgcggcggt
     3541 gatttatatc gtctccacca acgtgattgc cgggattgtg ccaaatatgg agctggcaaa
     3601 ttcaacggca ccatttggtc tggccttcgc gcagatgttc acgccggaag tgggtaaagt
     3661 cattatggcg ctgatggtga tgtcctgctg cggttcgctg cttggctggc agttcaccat
     3721 tgcccaggtg tttaaatctt catctgatga aggctacttc cctaaaattt tctcccatgt
     3781 aaccaaagtg gatgcaccgg tgcagggaat gttgaccatt gtgattatcc agagcgggtt
     3841 ggcgctgatg acgattagcc cgtcgctaaa cagtcagttc aacgtgctgg ttaacctggc
     3901 cgtggtgacc aatatcattc cgtatattct gtcaatggcg gcattagtca ttattcagaa
     3961 ggtcgctaat gtgcccccct caaaagcgaa agttgcaaac tttgttgctt ttgttggcgc
     4021 gatgtacagc ttttatgcgc tgtactcatc cggggaagaa gccatgctgt acggttccat
     4081 cgtgaccttc cttggttgga cactgtatgg tctggtatca ccacgctttg aactgaaaaa
     4141 taaacacggt taaaaaagca aagtaaaaaa gggcgatctt gcgaccgccc tttttcttat
     4201 ttaatgtgtt tacgcgtttt tcagaacttc gctaacaatc tcaaccgctt ctttctcaat
     4261 ctgcttgcga tgttcttcac cgaggaagct ttcgcagtag atcttatatg cgtcttccgt
     4321 gcctgacgga cgcgcggcga accagccgtt gtcagtcatc actttcagac cgccaataga
     4381 agcaccgttg cccggagcag cagtcaggcg cgcggtgatc gggtcaccag ccagggtgct
     4441 ggcactcacc atttccggag acagcttaga cagcgccgct ttttgtgcgg aagtcgcagc
     4501 tgcctgcaaa cggttgtagc tcggcgcacc aaagcgtttt gccagttcgt tgtagtgttc
     4561 ctgcgggttc ttaccggtga cagcggtgat ttccgccgcc agcagacaca tgatgatgcc
     4621 gtctttgtcg gtggaccacg gcgtgccgtc gaaacgcagg aaggaagccc ctgcgctctc
     4681 ttcgccgcca aagccgaagc tgccgtcaaa cagaccatca acaaaccatt tgaaacctac
     4741 cgggacttct accagtttac ggcccaagtc gttgaccaca cggtcgatca tcgcagatga
     4801 aaccagcgtt ttaccgacgg caacagcttt gccccactgc ggacgatgct ggaacaggta
     4861 gttgattgcc accgccaggt agtggttcgg attcatcaaa cctgccggag tgacgatacc
     4921 gtgacggtca taatccgggt cgttagcaaa cgccagatcg aacttatcac gcagtgccag
     4981 caggcccgcc atcgcacact cggaggagca gtccatacgg atcgcgccgt ctttatcaag
     5041 gtgcataaag cggaaggttt gatcgacctg atcgttaacg atggtcaggt tgaggttgta
     5101 atactcgcca atacgcttcc agtattcgat accggaaccg cccagcggat caacgccaag
     5161 cgtcaggccc gctttctgaa tcgccgccat atcaacgata tcggccagcc cttccacaaa
     5221 cggctgcacc agatcctgct ctttcacatg accggatgcc atcgcttcgt cgagggagat
     5281 acgcttcacg cctttcaggc catcggccat cagtgcgttg gctctgtctt ccaccacttt
     5341 agtgacgttg gtatcagccg ggccaccatt tggcggattg tacttgatac caccatcttc
     5401 cggcgggtta tgggacggtg taataacgat accgtctgcc agcgggccac cttttttatt
     5461 gtgaaccagg atggcattgg aaacggcagg cgtcggggtg aagccattgt tttcctgcac
     5521 aataacatct acgccgttcg ctgccagcac ttccagaacg gaaataaatg caggttcgga
     5581 cagggcgtga gtatctttac ccacatagca agggccagtg atgccgtttt tcgcacgttc
     5641 ttcagcaatt gcctgagcga tcgccagaat gtgcggctcg ttaaagctgt ggcgcgctgc
     5701 actgccacgg tgaccggaag taccgaattt caccgcgtgc tccgcattcc ctgcttctgg
     5761 tttcagtaca taatattgcg ccgtcagttg ggcgacgtta atcaaatcac tctgttgtgc
     5821 aggttggcct gcacgattgt ggattgccat tgctttgtcc tttgtctgca acgttttaga
     5881 tagttccgca aaccttctca atcaattccg ccgggaattg catcgactgc atgatgtgtt
     5941 cgatcatgct gcatttacgg ccggtgttgg tgttggtgat cacccaatac ggcgtgcctg
     6001 gcacatgttt cggcttggtc tgattaccat ttttcagcag cgtttgttca tctgccgcaa
     6061 agtaaacgcg tgtacgaccg tgcaacgatt ccgttgcttc ggcaaacgcc tgggcgtcaa
     6121 gagaatatag tgtagacaac agcagcataa agcgattgac cgctcgcttt tgctctgcat
     6181 attcatccga aagcagaagt tcacgcattg cgcgaacctt gtctttaatc gttttgatcg
     6241 gcttcgcttc gacgatagca ggtgacgcaa cgcgaacctc tttcgtcacc ggagcagcag
     6301 gctgtgatgc ggcggaaaat ttcaacatac gccgtaaaat gtcggatgcg ctctcgccga
     6361 tatgcttagt gtggctggca atatagctgt agagttcatc atcaacttca atcgttttca
     6421 tcttaatcca gtgcagtgtc ttagctgaat acaagtcgcc aggagtatat gaataaatcc
     6481 cggcagcgga tagcgtcaag cctggcttgc agaaaaactc ggaataaacc ttatttgttg
     6541 cagccctgtg gcacaatggt caacataata ccctaacccg acaacgcgaa aaaaagaact
     6601 ttgccatgaa attgaatatc cgcgcgcaaa ctgcacaaaa ccagcacaat aattctccca
     6661 tcgttcttgt ccatggtctg tttggcagcc tcgacaacct tggcgtactg gctcgcgatc
     6721 tggtaaacga tcacaatatc atccaggttg atatgcgtaa ccacggtctt tcaccgagag
     6781 atccggtaat gaattacccg gcgatggcgc aggatcttgt tgatactctg gatgcacagc
     6841 agatcgacaa agcaacattt atcgctcact ccatgggcgg taaagcggta atggcactta
     6901 ctgcactggc ccccgatcgc atcgataaac tggtggcgat cgatatcgca ccggtcgact
     6961 atcacgtacg ccgtcatgat gagattttct cggctatcaa cgcggtcagt gaatcggacg
     7021 cacaaactcg ccagcaagca gcagcaataa tgcgccagca tcttaatgaa gaaggggtga
     7081 ttcagtttct gttgaaatct tttgttgacg gggagtggcg ctttaacgtg ccggtattgt
     7141 gggatcagta tccgcatatt gtgggttggg agaaaatccc ggcatgggat caccctgccc
     7201 tgtttatccc tggtggtaat tctccgtatg ttagcgagca gtaccgtgat gatttactgg
     7261 cacaatttcc acaggcacgg gcgcatgtga ttgcaggcgc gggtcactgg gtccatgctg
     7321 aaaaaccgga tgcggtatta cgcgctatcc gtcgctatct caatgattaa cgcaactgat
     7381 taaaaaccca gcgtccgcgt cgctttccgc gcgtgggcgt tggcgtgact catcgcgctg
     7441 atgtattatg gcgcgctatc catccgggca gaggcctggc tgattgtttc ccccgaagtc
     7501 accaagatca tggccaaaga acaaacggac cgtacgacat tagatctgtt cgcgcacgag
     7561 cgtcgaccgg gacgaccgaa aacaaatccg ctttcgcgcg atgaacagct gcgtattaat
     7621 aaacgcaacc aactaaaacg cgacaaagta cgtggcctaa agcgtgtcga actgaagctg
     7681 aacgcggaag ctgtcgaggc gctgaacgag ctggcggaat cgcgcaatat gagccgtagc
     7741 gaactgatcg aagagatgtt aatgcagcaa ctggcggcgc tgcgtagtca gggcatcgtt
     7801 taaatttcca ctttcatgta gcacagtgtg cagtcctgct cgtttgctga ttccgcaaga
     7861 ctgcctgttc tgctatgatt gcctttatcc gtgggcaatt ttccaccccc atttcaataa
     7921 gtttcaagag gttatttcac tcatggctat cactggcatc tttttcggca gcgacaccgg
     7981 taataccgaa aatatcgcaa aaatgattca aaaacagctt ggtaaagacg ttgccgatgt
     8041 ccatgacatt gcaaaaagca gcaaagaaga tctggaagct tatgacattc tgctgctggg
     8101 catcccaacc tggtattacg gcgaagcgca gtgtgactgg gatgacttct tcccgactct
     8161 cgaagagatt gatttcaatg gcaaactggt tgcgctgttt ggttgtggtg accaggaaga
     8221 ttacgccgaa tatttctgcg acgcattggg caccatccgc gacatcattg aaccgcgcgg
     8281 tgcaaccatc gttggtcact ggccaactgc gggctatcat ttcgaagcat caaaaggtct
     8341 ggcagatgac gaccactttg tcggtctggc tatcgacgaa gaccgtcagc cggaactgac
     8401 cgctgaacgt gtagaaaaat gggttaaaca gatttctgaa gagttgcatc tcgacgaaat
     8461 tctcaatgcc tgatgtgatg cggcgtagac tcatgtctac gccgtattaa tagataatgc
     8521 caatcaaaat aattgctaca aatttgtaac ttttgccgtt gtacctgtac aatgtcccgg
     8581 tgttcaagtg gccttgccgt tgtaaatgta agctgtgcca cgtttttatt aacaatattt
     8641 gccagggact tgtggttttc atttaggcgt ggcaattcta taatgatacg cattatctca
     8701 agagcaaatt ctgtcacttc ttctaatgaa gtgaaccgct tagtaacagg acagattccg
     8761 catgactgat aacaataccg ccctaaagaa agctggcctg aaagtaacgc ttcctcgttt
     8821 aaaaatcctg gaagttcttc aggagccgga caaccatcac gtcagtgcgg aagatttata
     8881 caaacgtctg atcgatatgg gtgaagaaat tggtctggct acggtatatc gcgtactgaa
     8941 ccagtttgac gacgctggta tcgtcacccg ccacaatttt gaaggcggta aatccgtatt
     9001 tgaactgaca cagcaacatc accacgatca cctgatctgc ctcgactgcg gcaaggttat
     9061 cgaatttagt gatgattcca tcgaagcgcg tcagcgtgaa attgccgcaa aacatggcat
     9121 tcgcctgact aaccacagtc tctatcttta cggtcactgc gccgaaggcg attgccgcga
     9181 agatgagcac gcgcacgaag gcaaataagc cagtctgaac gagaaaagcc aacctgcggg
     9241 ttggcttttt tatgcaaggg aaataagaaa agcgagagtt acagctctca cttatttgtt
     9301 attactgcga atttcctgcc agaccttatc acaatcggct ttcaccgcct gatcatttcc
     9361 ggtttgcgtt gcacgcagac actgctgata atccagtacg cggacacttt cctggtcggc
     9421 aaactgctga tgttgtttct ctttcttcag cacgtttaac acgctctggc aggcttcaat
     9481 tttttccggc gaaccttgtg cggtgttgat acaggcgctg tatgcctctt tcagacggct
     9541 gtcttcctgt ggcgcggttg attgcgcaca agctaccagc cccgatgcca tcatggcgat
     9601 gagaatcagt tttttcatag caaattcctc aaacgtgcgg caggcacaaa acctgccgcg
     9661 tcgggcatca gaagatggtg aatggtgcga ttaccataaa ttttacgtca cgctcatcct
     9721 ggaagatgtt gccgtaaccg ccgccccagc ttgggatgtc ggagtggttg tcgtattcgg
     9781 tgaagtgcag tttgaacatc gtgcctttgg cgcgaccgtc ctgaatggtg tagaccgcat
     9841 ccaggctgta tgcagactct tcaatagtcc ggtttttgtc gtagtacgca tccggattgc
     9901 tctgccaggt cgcaggttta gcatcccatg cgtaaacgta ggaagcgccg atggcgaagc
     9961 ctggaagatt ccagtttttc aggtcataca tcgcaccgaa gaagaccgct ttttcgccgt
    10021 tggcgttgaa gtcagaacgg ttatcccacc agatatccag gcgaccgttc gaggaagcgt
    10081 aggttggagt catacgttgc aggaagtatc cctgctgacc gtcagcttta acccaggtgc
    10141 cttcgaggcg caaatccact acgtcagccg cccggtaacc aaaggtcaac gcttgcagcc
    10201 aggcggtgcc gtcataaaga tcgttgacgc tgcgatcgtc aactttatcg cgcgtaccgt
    10261 agaactggta gctggtggtt aacgggctac cggcgatatc aaatttgtag ctggctttgg
    10321 caaagtattg atcgatatac ccttccgcct gaccaaatgc cgcttccagt acgaagttat
    10381 ttttgaagtc gtatttcgcc ccaagggagt gcagataatc aactttggtg gttttatcgt
    10441 tctgataaaa ctcatccatt tccagatgcc acggtgcttt gtattcgttg gtccacatgt
    10501 aggagaaact caacgcacca gcatcgccgt aatcaaaatt cgccccggcc tccgcaccct
    10561 gataagtacc cggcataaag ctccagtgcg gcgctaacag cgtttgacca gttggctgaa
    10621 tataacctgc cctcgccaca ccggaccgta tttaaatttg gccgctgctt tatacaggct
    10681 tataccgctt ttatcgccgg accagtcttc gtcataggct ttattacttt ttgaaaacgc
    10741 gatttcgttc gggtgagagc tgtcgccgtt ttccgccatt tcaatcgccg taaacgcggc
    10801 aatatcaagg ccgaacatat cagcagcata accggactga aaatcgaggt tggcgttcca
    10861 ggtggagtga gaaaggttgg ttttgtattt atcgccgtcg gtaacatctt tacggtcgcg
    10921 ttcacgctgc cagtaataga taccgccggt taaggttgaa tcgtcaatga atccttcggc
    10981 gcgagcctcc ggcattgcca taaagcccga cattgctgta acaccggcga tagccagcgc
    11041 cagcgtacta cgtttgccac taaacgtacg catgggttaa tcctctttga cgtataaatt
    11101 gctgcaccac aggtgaacag cgaaaaaaat aaaaattaaa aaagtagcgt tgagaaaagt
    11161 ctcgctgact acaggaacag actatttttc gcgacgcgaa tt
//
LOCUS       DBFIFI010000140        11194 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.140, whole genome shotgun sequence.
ACCESSION   DBFIFI010000140 DBFIFI010000000
VERSION     DBFIFI010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11194
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.140"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..482)
                     /gene="sulA"
                     /locus_tag="N7B39_003441"
     CDS             complement(<1..482)
                     /gene="sulA"
                     /locus_tag="N7B39_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HGU2423361.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     L"
     gene            701..1330
                     /gene="sxy"
                     /locus_tag="N7B39_003442"
     CDS             701..1330
                     /gene="sxy"
                     /locus_tag="N7B39_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HGU2423362.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKYPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(1293..3455)
                     /gene="yccS"
                     /locus_tag="N7B39_003443"
     CDS             complement(1293..3455)
                     /gene="yccS"
                     /locus_tag="N7B39_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HGU2423363.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(3465..3911)
                     /gene="yccF"
                     /locus_tag="N7B39_003444"
     CDS             complement(3465..3911)
                     /gene="yccF"
                     /locus_tag="N7B39_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HGU2423364.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIALIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            4034..6088
                     /gene="helD"
                     /locus_tag="N7B39_003445"
     CDS             4034..6088
                     /gene="helD"
                     /locus_tag="N7B39_003445"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HGU2423365.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVLIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(6120..6578)
                     /gene="mgsA"
                     /locus_tag="N7B39_003446"
     CDS             complement(6120..6578)
                     /gene="mgsA"
                     /locus_tag="N7B39_003446"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HGU2423366.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(6674..7336)
                     /gene="yccT"
                     /locus_tag="N7B39_003447"
     CDS             complement(6674..7336)
                     /gene="yccT"
                     /locus_tag="N7B39_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HGU2423367.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            7509..7922
                     /gene="yccU"
                     /locus_tag="N7B39_003448"
     CDS             7509..7922
                     /gene="yccU"
                     /locus_tag="N7B39_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HGU2423368.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(7967..8284)
                     /gene="hspQ"
                     /locus_tag="N7B39_003449"
     CDS             complement(7967..8284)
                     /gene="hspQ"
                     /locus_tag="N7B39_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HGU2423369.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(8342..9532)
                     /gene="rlmI"
                     /locus_tag="N7B39_003450"
     CDS             complement(8342..9532)
                     /gene="rlmI"
                     /locus_tag="N7B39_003450"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HGU2423370.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            9627..9905
                     /gene="yccX"
                     /locus_tag="N7B39_003451"
     CDS             9627..9905
                     /gene="yccX"
                     /locus_tag="N7B39_003451"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HGU2423371.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(9902..10231)
                     /gene="tusE"
                     /locus_tag="N7B39_003452"
     CDS             complement(9902..10231)
                     /gene="tusE"
                     /locus_tag="N7B39_003452"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HGU2423372.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(10322..10981)
                     /gene="yccA"
                     /locus_tag="N7B39_003453"
     CDS             complement(10322..10981)
                     /gene="yccA"
                     /locus_tag="N7B39_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HGU2423373.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKMANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
BASE COUNT         2940 a         2752 c         2907 g         2595 t
ORIGIN      
        1 agcccggaaa gttgtctcgt ggcgtgagag gatgcgctta ccggacgcat aataaacccc
       61 atagcgttac cttcatttgc cgcatcaaca agttcagcat gctcttcttc agtcaaatca
      121 tctgccaacc aaccgatcac cacactgtaa ttgcccgtgc gtaaagcgcg aaccattgac
      181 tccacagtgt ggcaagggga gagctggcta atctgcatta ctttcgttaa gggtagccca
      241 gatgcctgaa cccattcccg actcagtttt tgttgcggtg ttaaccagag ttgccagcgc
      301 gattgctgac cgagttgctg taacaatggc aacagtagaa gttgcgtcat catgggctga
      361 tcttcgcgat agacaacttc actgataagc ccggctgtag tgttttccgt agagacacgc
      421 gcaattttac ttgctgcgga tgagaacgac gaagaacgat gtgcatagcc tgaagtgtac
      481 ataatcaatc cagcccctgt gagttactgt atggatgtac agtacatcca gtgacaacaa
      541 agatcaaccc tattttcgga aagagcctcg caaattttgt cgttggtgac gggaaaacat
      601 aaattaatct tgccgcttaa gaataagttg cctattttcg tagttaacgg atccgttaat
      661 gtgaatcatt cttttatgtt atgattttaa aaggaatttt atgaaaagcc tctcctataa
      721 gcggatctat aaatcacaag aatacctggc aacgttgggc acaattgaat accgatcatt
      781 gtttggcagt tacagcctga ccgttgacga cacggtgttt gcgatggttt ctgatggtga
      841 gttgtatctt cgggcttgtg agcaaagtgc acagtactgt gtaaaatatc cgcctgtctg
      901 gctgacatat aaaaagtgtg gccgatccgt taccctcaat tactatcggg ttgatgaaag
      961 tctatggcga aatcaactga agctggtgcg tctgtcgaaa tattctctcg atgcagcgct
     1021 gaaagagaaa agcacgcgca atacccggga aagactgaaa gatttgccca atatgtcttt
     1081 tcatctggaa gcgattctcg gggaggtggg gattaaggat gtacgggcgt tacgtatact
     1141 tggggcaaaa atgtgttggt tgcgactgcg gcagcaaaac agtctggtga cagaaaagat
     1201 tctgtttatg cttgaaggtg ccattatcgg cattcatgaa gctgcgctcc cggtggcacg
     1261 ccgccaggag cttgcagaat gggctgactc tcttacgccg aaacaggagt ttcctgcgga
     1321 acttgagtaa tctggcgttg cagacgacca atctcaggca gtaatgcaat caatagtcca
     1381 acttgttgta cgaccagagg ttctttgctg tctgcccgtg gttcaagttg ctgcatccgc
     1441 tgtttcaggc tagctaatgc ctcattgacg cgttcttcat cagcaggttg atgatgtaac
     1501 gcgtcatcaa catagcaaac tgcgtcatca agaaacgcca gaatttcagg attagttaac
     1561 tgctcccggt gagcaccgag ggctgagata tagctggtaa acgtatggtt aaggcacagc
     1621 aaccgaaacg cggcttcgcg aatttgcggg gtaacgttcg gctcgctgga catatttgat
     1681 accaccgacg ccagctcagc atcacggttg tgtgcatcgc ggcgggcaat acgatacgcc
     1741 agacggttat cacgcccctg atggtattgc tccagtatgg catcgagata ccgacagttg
     1801 gcctctgtgg cgcgttcgag catgcgcggc agattgcgaa actgccagtc aggccagatg
     1861 tagctcactg ccgcccacgc aatggcacaa ccaatcagcg tatcgattac gcgaggtaac
     1921 gctacttcaa aaccttcacc cagtaagtta aaacacagta gcaccaaaag tgtgatgaac
     1981 atcgttgcat gagcgtattg cacgttacgg aaggcaaaaa agagcacgcc ggtaataacc
     2041 agcagcacca gctgcccttc cagtgatggc acaaaccaca gcacaggaat gccaatggcg
     2101 atacctacca gcgtaccaat aatccttaac ttcaggcggt ggcgcgtggc gttatagttt
     2161 ggctggcaga caaacaaact tgtcagcaag atccaatacc cgtgatgcat tccggttatc
     2221 tgaatgatgg cgtagccgaa gcacaacacc agcgacattc ttaccgcatg acggaagagg
     2281 gcggattccg gcgtgaagtg acggctaaga cgcagccaga tatcactcaa cccgtgcggg
     2341 ctgtcatcag cgagctcatt ttcgtcatta ttatggggta gtgcctgggc ctgttctgat
     2401 tcaattgttg ccagttgggc atcaatggcg cgtaaattgt tcagcaaaaa tcccagtgtt
     2461 ttgagtaaat cggcgggtgc gccgttatcg cgcatccgct ccagcgcagc atcaatatgc
     2521 gtaaaagcgc gctcaaaatg cggatcatgt tgataaggct gacgcaacaa aatacagcgt
     2581 gacagttgct ggcacgcctg gccctgcatc gacatcagcc gctgaaaacg gaacagcacg
     2641 tcgctgtggc gaaaatgttc acgcaatgtt tgatactgaa tatgagaaga gctggcacgc
     2701 tcgtgaatat cctgtgcgac aaagtaataa tgcagcgtgc gacgcgttcc ccgttgacca
     2761 cgatcgccac gtaagcgggt cagcagcgag agtttcgtct gattcaatgt cgccatcagc
     2821 agaccgttgg cgagagccaa atcgtacagc ggtgcctggc tttcatcttc aatatcagga
     2881 tcaaacatgc gcgacttgag ctcaagataa cgcgccagtt gttcatagca acgcgccagg
     2941 ttgtcctgca gcgggcggac cgggaacagc agatgaccaa taagtgtcag gacgttgtac
     3001 cagacggcac cggccagcag atacatcggc tgctgatacc agtgctcata cagtgatgtt
     3061 cccaacatag tgtaaatggc gatcagcaat gcaccgaagg caattgttgc atagcgttga
     3121 cccagaccgc cgagcaaaat gaagccgctg gtagagagcg ttaagccaat cgcaaatagc
     3181 cagggccagg gaaacagcaa ttctactgag gccgaggcga taaaaaagca gaacagcgta
     3241 atgatgaggt tacgcaaacg tcccgccagt cggtcatcga gatcggtcag cgccgctgcc
     3301 accatcccca gcgttagcgg aatcgtcagt tttacatcac ccagccacca cggaaacgct
     3361 gtggttccac aaagcgcaat aaaaatacgc gcgtaataca gccaggcgct gttccaggta
     3421 tagcgtttga gcaaaggact tagcataaag gccatattag tggattattc aaaacgacga
     3481 cgtgcattgg cttcacgcgc agcttgcgct gtttctaccg ataccacgcg acgaccaacc
     3541 ggccatagtg caatagcggc aattttaaag ttcgcaatgc cgacaggaat gccaatgatt
     3601 gaaatacatt gtgcgatgcc cgttgcaatg tgcatcaggc ataaccacca gccaaagaaa
     3661 atcagccaga aaatattcaa taccgtaccg ccagtattca gcagcacatt tttgccagcc
     3721 gggttcagtt catcgacatg aatagcttca ttgccataag gcaccagaga cagtttagtg
     3781 atctcccagc aggatcgtgt cagcggtaag gtaaaaatca gcgcaatact gaccagagtc
     3841 gccaacagcc agcccagagt ggtggcaaat ccgccaagca caaagttcag aatgttcaaa
     3901 acggtacgca taaaacctcg ctttactgtg gttttcagta atgggcggca attgtaacgt
     3961 ttttttgggc tggagcacgt tttctctgac ggttacactg ataagaaata atttcgtgtg
     4021 gatctacaga gtcatggaac tgaaagcgac aacgcttgga aaacgtctgg cacagcaccc
     4081 ttacgatcgg gcggtgatcc tcaatgccgg gattaaagtc tccggcgatc gccacgaata
     4141 ccttattcct ttcaatcaat tactggcgat tcactgtaag cgcggtctgg tatggggcga
     4201 gctggaattt gtactgccgg acgaaaaagt ggtgcgtctg cacggtaccg aatggggcga
     4261 gacgcagcgt ttttaccatc atcttgatgc tcactggcgg cggtggagtg gcgagatgag
     4321 cgaaattgcg tctggtgttt tacgccagca actggatttg attgccacgc gcactggaga
     4381 aaataaatgg ctgacgcgtg agcaaacctc tggcgttcag caacaaatcc gccaggcttt
     4441 gtcggcgttg ccgttgccgg ttaaccgact ggaagaattc gataactgcc gtgaggcgtg
     4501 gcgtaaatgt caggcctggt tgaaagatat tgaaagcgct cggttgcagc ataaccaggc
     4561 gtataccgaa gccatgctta ccgagtatgc ggattttttc cgccaggtcg agtcttcacc
     4621 gctgaatccg gcgcaggccc gggcagtcgt taatggcgag cattctctgt tagtgctggc
     4681 aggtgcagga agcggaaaaa cgtcggtgct ggtggcccgt gcaggctggt tgctggcgcg
     4741 tggtgaagcg tcccctgagc aaattttatt gctggcgttt ggtcgcaaag ccgctgaaga
     4801 gatggacgag cgtattcgcg aacggctaca taccgaagac attaccgcac gcacgtttca
     4861 tgcgctggcg ctgcatatta ttcagcaggg cagcaaaaaa gttccgatag tcagcaaact
     4921 ggaaaatgat accgctgccc gtcatgaact ctttattgct gagtggcgca agcaatgcag
     4981 cgaaaagaaa gcgcaggcga agggctggcg gcaatggctg acggaagaaa tgcagtggtc
     5041 agtgccagaa ggtaacttct gggatgatga aaaattacag cgtcgtcttg cctcacgcct
     5101 cgatcgttgg gtaagtctga tgcggatgca cggtggtgca caggcagaaa tgattgccag
     5161 tgcacccgaa gagattcgcg atctgttcag taaacgtatc aagttgatgg ccccgttatt
     5221 aaaagcctgg aaaggtgcgc tgaaggcaga aaacgctgtc gatttttcgg gccttattca
     5281 tcaggcgatt gtgattctgg agaaaggtcg ctttatcagc ccgtggaagc atattctggt
     5341 tgatgaattt caggatatct cgccgcagcg ggcagcgttg ttagcggcat tacgcaagca
     5401 aaacagtcag acgacgttgt tcgccgttgg tgatgactgg caggcgattt accgattcag
     5461 cggtgcgcaa atgtcgctca ccaccgcttt ccatgaaaac tttggtgaag gcgaacgctg
     5521 tgatttagac acgacttacc gttttaacag tcgtatcggt gaggtggcaa accggtttat
     5581 tcagcagaac ccaggccagc tgaaaaagcc gctaaacagc ttaaccaatg gagacaaaaa
     5641 agccgtcacg ttattggatg agagtcaact tgacgctttg ctggataagc tctctggtta
     5701 tgccaaaccg gaagagcgca ttctgatcct ggcgcgttac catcacatga ggcctgccag
     5761 cctggaaaaa gcggcaacac gctggccgaa gttgcaaatc gactttatga ccattcatgc
     5821 cagcaaaggg caacaggcgg attacgtcct catcgttggc ttgcaggagg gaagtgatgg
     5881 ttttccggct gcggcgcggg agtcgattat ggaagaggcg ctactgccac cggttgagga
     5941 tttcccggac gctgaagaac ggcggttaat gtacgtggcg ctgacccggg cacgtcatcg
     6001 ggtatgggca ctgtttaaca aagagaatcc ctctcccttt gtggaaatac tgaaaaatct
     6061 ggatgtgccg gtggcgagaa aaccgtaaga aacaggtggc gtttgccacc tgtgcaatat
     6121 tacttcagac ggtccgcgag ataacgctga taatcgggga tcagaatatc gaccgcgtcg
     6181 ttgaaatgcg gcgactggat aatgaagtct gccgttgcca cgttggtggc gaccggaatg
     6241 ttccataccg tcgccagacg cagcaaggct ttcacgtcag gatcgtgcgg cacggcattt
     6301 agtggatccc agaagaaaat caatacatca attttccctt ctgagatcaa tgcgccaacc
     6361 tgctggtcac cccccattgg gccactcaac atcgcgttga cgttcatgcc ggtcgcgcgg
     6421 gaaattaagt taccggtagt gcctgttgca tacagtacgt gttgttccag taacggttga
     6481 tgccgttcca cccagctcat cagcatttgt ttgcagtgat cgtgtgccac cagcgcaata
     6541 tgtttccgcg caggtaaagt gcgagtcgtc agttccataa tgtacatccg tagttaactt
     6601 tcctacagat tactgtaagc acttatcgct gcaagataaa gaccgaaaaa gcctgcgcac
     6661 aggcacaaaa atctcaggaa gatggttgtt tttccgccca ttgcaggaaa gtatttcgcg
     6721 tttgtgggtc agccagttta aaccaatact tcagccgttg ttctgtgagc acctgagact
     6781 gcggtggaat agcatccagc tcggaaacac ccgaaagcag cgtactgtca tctgccatca
     6841 tcgtggcaaa ttgcggtagt gaagcgcgtt tcgcggattt gttatagcgt tcaacctcga
     6901 cctcataatc aatcgttttt gccgttgagg taatggcgag aatatccagc tttaccggaa
     6961 tcggcgtcgc atcgccatcc aacaattcaa ggcgcggcgc ggcatcaaaa tggttagctt
     7021 cccgctcatt ctccaggcga ggcaggcgaa aattgacctg gttgatgagc tgggtattaa
     7081 aactgaccac cagcggtggg gagatataca gccgttcttc actattggaa agatgaatcg
     7141 tcttctcaac gcgaaacact aactgatgcg gtccattatc cagttcaatg ctgtccgcac
     7201 cgcgcagcag agagctggaa accttcttgc cgtcgagtac cagaagatcg acatcggttg
     7261 aaagccgcag cgtggtggca aaaacggata ccggcaaaca taatgcaatc aaggtggtca
     7321 cgatgccggt tttcataggt agctcctgtc aaaagaccgc ccgaataata atgtgtcaaa
     7381 atttttcttt aatcatactt actaacatat agacatattt cccgcttttg ctctcattca
     7441 ttcgtattag ctgcatggtt ggcatgtcgg ctttgtcgta cactttgtga aacagtctgg
     7501 aggaaataat gaaagaaacc gatattgcag gcattttaac gtccactcac accattgcgc
     7561 tggtgggggc aagcgacaaa cccgatcgtc ccagctatcg cgtgatgaaa tatctactcg
     7621 accaggggta tcacgtcatt ccggtttcgc caaaagttgc cggcaaaacg ctgctgggcc
     7681 agcaggggta cggcacgctg gcagacgttc cggaaaaagt cgatatggtc gatgttttcc
     7741 gtaattcaga agcggcgtgg ggcgtagcgc aggaagctat cgccattggc gcgaaaaccc
     7801 tctggatgca actgggggtg attaatgagc aagcggcagt actggcacgg gatgccgggt
     7861 tgaatgtggt gatggatcgc tgcccggcta ttgagatccc tcgcctgggc ctggccaaat
     7921 aaaaaatccc cggaaggcaa aaaccttccg gggatctgtt cagggattag ttacgcagac
     7981 gcggggcctg gagttgtttg cggatggtct gcgccagctc atccatggat ggctgttcag
     8041 gatgttcatc ctgcaattcg ctacttagct gggcttcagc caggtaggta tgaaccggta
     8101 ggccgttatc gtcctccatc accacgtgat accacggagc ggcgcgaagc tcgtcattca
     8161 ccgccagctc atcaggcgac ggttcagaga gcgaataaac cgggtcgata tccacgacca
     8221 ctccgagata acctaacagg gaatggcgga cctgctggcc gataccgaat ttgctggcaa
     8281 tcatagtcac ctcccgggaa atctttacct ttgatataag ggtaaaattc cacatttcaa
     8341 gttacatgac gcgacaggca aaccctttca gatatagccc ttccggatag gtagcgatca
     8401 ccggatgatc ggccgcctga cggaactgct ctataaattg tacatcacga ccggcatcaa
     8461 ttgcggcatc cgcgatgatt ttctgaaata aatcgctggt catcagaccg gaacaggaga
     8521 aagtcaggag aataccgcct tcattcagca gctgaatcgc cagcatgttg atatctttat
     8581 agccacgaca cgcgcccatc aactggcttt tattctcaac aaacttcggc gggtccatca
     8641 cgataacgtc aaatttttca ccgcgatcgc gataggtacg cagcaattta aagacatcat
     8701 cacggacaaa ctcagccttg ctcagatcca gtttgttcag ctcaacgttc tgccgtgcaa
     8761 tatccagtgc ttcctgggag gtatcaacgc tgacaacctg gctgcaaccg cccatcagtg
     8821 ccgatacggc gaaaccaccg gtataggaga aacagttcag tacgcgttta ttttcaacgt
     8881 agcggcgggt agccaggcgg ctgtctcgct ggtccaggta gtagcccgtt ttgtgtccgt
     8941 gctgtatgtc caccagcagc ttcatgccat gttcttcaat cggcagcagg gcaggcggca
     9001 actcaccggt gacgaggccc tgggtcagct ccatcccttc ttttttacgt accgcaacat
     9061 cgctgcgatc gtaaatcgca cattccgggt acagcgtttg cagggcacta attaatgccg
     9121 cgcgctgata ttctgcgcca gcactcagca gttgcagcac cagaaaatta ccgaaacgat
     9181 cgatagtaat acccggcagg ccatcagatt ctccggcgat taaacgatag ctgtcgaggc
     9241 catctttttg cgccagccag tcacgccatt tttgtgcttg ttgcaaacgg cgggaaaaaa
     9301 aagcaatgtc gatagactca gacgggtcaa acgtccagac gcgcgcccgg atttgcgaag
     9361 ctggcgaata agcgccgcgt gctaaccatt ttccctgatg atcaacaata tcgatggttt
     9421 caccgaggct ggctttacct tccatacggg caacggcccc ggaaaagacc cacggatggc
     9481 gacgaagtaa tgatttttcg cgccctttgg ctaacactaa acgtacactc ataatttact
     9541 tttctgtcga tgcaaagaaa tgggcgtcat tgtccggagt tttccatgga tttgcaacgc
     9601 gtccattaag gataaggaga gcagcaatgt cgaaagtctg cataattgcc tgggtttacg
     9661 ggcgagttca gggcgtagga tttcgctaca ccacacagta cgaagcgaaa agactgggct
     9721 taaccgggta cgccaaaaat cttgacgatg gcagcgtgga agtggttgcc tgcggtgaag
     9781 aagggcaggt agaaaaatta atgcagtggt taaagagtgg cggcccgcgt tctgcgcgtg
     9841 tagaacgggt gctcagcgag ccgcatcatc cctcggggga attaaccgat tttcgtatcc
     9901 gttaaataca ttttaccggt tttggcaggc cagcaatttt ggtggcttgc ttagccggac
     9961 ctttcggaaa cagtcggtat aaatagcggc tgttaccttt ttcttcgcca aatttattcg
    10021 ccatcgcttt taccagcata cgaatcgccg gagaagtatt gaattccaga tagaaatcac
    10081 gcacaaaacg caccacttcc cagtgttctg gcgacagcga aatcccttcg ttctctgcaa
    10141 tcaccactgc cagcggctca ctccactggc tgctttcttt gagatagcct tcggtatccg
    10201 tttctatctc tttaccttcg aagatcagca taattattac taccttaatc agactgccgg
    10261 cagtgtaaca aaaaacaaag ccccgcataa agcgaggcta tgaaagtgtt agcgggtgag
    10321 attaatcgcg gctggcgaag cccagaatgc tcagcaggct gacgaagatg ttgtacagcg
    10381 aaacatacag gctaaccgtg gcacgaatat agttcgtttc accgccatga atgatgttgc
    10441 tggtttcaaa caaaatagcg ccagaggaga tcaaaatgaa gaccgcgctg atcgccagat
    10501 gcagagcagg cagctgcagg aagatattag caaccatacc aatcagcacc accacaatac
    10561 ccgccatcag cataccgccg aggaacgaca tatctttgcg ggtggtcagc acatatgcag
    10621 agcagcagaa gaacactaac gccgttccgc ccagtgccat agcgattacg tcacccattc
    10681 cggcagacag ataggtattc agaattggtc cgaggatata acccagaaaa ccggtaaagg
    10741 cgaatgcgga gataatcccg gtcggcttat tcgccatttt ataggtcagg aacatcaaac
    10801 catacatacc caccagcgtc agaatcagac ccggagatgg cagcatcagc accgtactgg
    10861 cagttgcggt aatcgccgaa aaggccagcg tcaggctcag cagaaaatag gtattacgca
    10921 gcactttatg ggtgctaagc agtgatgtac ggtcatgtga agaactaaca atacgatcca
    10981 ttgagtcact ctctatgaca gatgtaatta attaagcagc ataatgataa tgcgtaaggg
    11041 cacccagtag ttttacccat ctttacgcat ttgatctgga acaggtttaa cagcggatta
    11101 tcaggtcatt aagcaaatat aacgccctga gaatttcggc aggcaaaaga aaaaggggtt
    11161 agcatttagc taacccctta tcttatctgg cgga
//
LOCUS       DBFIFI010000141        11173 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.141, whole genome shotgun sequence.
ACCESSION   DBFIFI010000141 DBFIFI010000000
VERSION     DBFIFI010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11173
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.141"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            33..1121
                     /gene="yehL"
                     /locus_tag="N7B39_003454"
     CDS             33..1121
                     /gene="yehL"
                     /locus_tag="N7B39_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HGU2423374.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            1132..3411
                     /gene="yehM"
                     /locus_tag="N7B39_003455"
                     /pseudo
     CDS             1132..3411
                     /gene="yehM"
                     /locus_tag="N7B39_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
     gene            3404..4540
                     /gene="yehP"
                     /locus_tag="N7B39_003456"
     CDS             3404..4540
                     /gene="yehP"
                     /locus_tag="N7B39_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HGU2423375.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTPPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSSIPLARDFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRHSEQWQLV
                     LLVDLSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDMAQALVNVGAQIAAMTPGELATWLAENLQS"
     gene            4537..6540
                     /gene="yehQ"
                     /locus_tag="N7B39_003457"
     CDS             4537..6540
                     /gene="yehQ"
                     /locus_tag="N7B39_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HGU2423376.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANGQALKEAQCTCGASGMCRHRVMLVLSYQRLCATTRPTEKEE
                     EWQPAIWLEELATLPDATRKRAQVLVAKGITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVEAKTQQAEFTHLIWQMRSEHVTSSDDPFASEEG
                     NACRQYVQQLSQALWLGGISQPLIHYEAAFSRAQQAAERCNWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARRDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWRMLSAPLRQPGIVALR
                     EYLRQRPPSCIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAVERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVASLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAIGQAELLANDLTA
                     VGFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPLLQQQG"
     gene            6665..7126
                     /gene="yehR"
                     /locus_tag="N7B39_003458"
     CDS             6665..7126
                     /gene="yehR"
                     /locus_tag="N7B39_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HGU2423377.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(7168..7638)
                     /gene="yehS"
                     /locus_tag="N7B39_003459"
     CDS             complement(7168..7638)
                     /gene="yehS"
                     /locus_tag="N7B39_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HGU2423378.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(7685..8404)
                     /gene="btsR"
                     /locus_tag="N7B39_003460"
                     /gene_synonym="yehT"
     CDS             complement(7685..8404)
                     /gene="btsR"
                     /locus_tag="N7B39_003460"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HGU2423379.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(8401..10086)
                     /gene="btsS"
                     /locus_tag="N7B39_003461"
     CDS             complement(8401..10086)
                     /gene="btsS"
                     /locus_tag="N7B39_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HGU2423380.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYLELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISVRREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            10308..10373
                     /locus_tag="N7B39_003462"
                     /pseudo
     CDS             10308..10373
                     /locus_tag="N7B39_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10601..10849
                     /locus_tag="N7B39_003463"
     CDS             10601..10849
                     /locus_tag="N7B39_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HGU2423381.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVR"
     gene            complement(10860..10931)
                     /locus_tag="N7B39_003464"
                     /pseudo
     CDS             complement(10860..10931)
                     /locus_tag="N7B39_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299876.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
BASE COUNT         2724 a         2947 c         2855 g         2647 t
ORIGIN      
        1 ttatttcatt aatacatttc agggatgaat atatgtcacc gcagaataat catctgcagc
       61 gtccgcctgc tgctgtgtta tacgccgatg aactggcaaa attaaaacaa aatgataacg
      121 caccttgccc gcccggttgg cagttaagtt tgcctgcggc ccgtgctttt atccttggcg
      181 atagcgcgca aaatattagc cgtaaagtgg tgattagtcc ctccgctgtc gaacgtatgt
      241 tagtcactct tgccaccgga cgcggtttga tgttggtggg ggaacctggc accgcaaaat
      301 ctctcctgtc tgaattactg gcaacctcta ttagcggcga tgccgggtta accatccagg
      361 gcggcgcatc tactaccgaa gatcaaatca agtacggctg gaactacgcc ctgcttatca
      421 atcatggtcc atcaacggaa gcgctggtcc ctgccccgct ttaccaggga atgcgcgacg
      481 gcaaaatcgt ccgttttgaa gagataacgc gtacaccgct ggaagtgcaa gactgtctgt
      541 taggtatgct ttctgacagg gtgatgacgg tgccggaact cactggtgaa gccagtcagc
      601 tctatgcgcg agaaggcttt aatattattg ccactgccaa tacccgcgat cgcggcgtca
      661 atgaaatgag tgctgcgcta aagcgtcgct tcgattttga aactgtcttt ccgattatgg
      721 atttcgccca ggagctggag cttgtcgcca gtgcttcggc gcgtttgctg gcccatagcg
      781 gtattccgca taaagtgcct gacgcggtac tggagttact ggtccgcacc ttccgcgatc
      841 tgcgtgccaa cggcgaaaag aaaacgtcaa tggatacact aacagcgatt atgtccaccg
      901 ctgaagccgt gaacgtggcc cacgctgtgg gcgtccgcgc ctggttttta gcgaatcgcg
      961 cgggggagcc tgccgacctg gtggactgta ttgccggaac catcgtcaaa gataacgaag
     1021 aagatcgcgc tcgtctgcgc cgttacttcg aacagcgtgt cgccacacat aaagaagctc
     1081 actggcaggc ttattatcaa gcccgccacc gcctgccgtg aggaaagatg catgagcgag
     1141 ccgttaattg tcggcatccg gcatcatagt ccggcctgcg cccgactggt gaaatcgtta
     1201 atcgaaagcc agcgaccacg atacgtgttg attgaaggcc cggctgattt taatgaccgg
     1261 gtagatgaac tgtttttagc ccaccagctt ccggtggcta tttacagtta ttgccagtat
     1321 caggacggtg ctgctcctgg gcgcggcgcc tggacgccat ttgccgaatt ttcgccggag
     1381 tggcaggcgc tacaagccgc acgtcatatt caggcacaaa cttactttat cgatttgcct
     1441 tgctgggcgc agagtgaaga agaggacgat tcgcctgata cgcaagagga aagccaggcc
     1501 ttgctgctgc gtgccacccg catggataac agcgataccc tgtgggatca cttgttcgaa
     1561 gatgaaagcc agcaaactgc attaccctct gcactggcgc actattttgc ccaactgcgg
     1621 ggcgatttcc ccggcgatgc actcaatcgt cagcgcgaag cctttatggc tcgctggatt
     1681 gcatgggcag tgcagcaaaa taatggcgac gtgttagtcg tctgcggtgg ctggcacgct
     1741 ccggcactgg caaaaatgtg gcgcgaatgc ccgcaggaca ttaacaagcc agaattgccc
     1801 tcgctggcag atgccgttac aggttgttat ctcacaccct acagtgaaaa gcgccttgat
     1861 gtgctggcag gatacctttc aggaatgcct gccccggtct ggcaaagctg gtgctggcag
     1921 tggggcttac agcaggcggg agaacaactg ctaaaaacgg ttcttacccg tttgcgccaa
     1981 caccacttgc ctgcttcgac cgcggatatg gctgccgctc atctgcatgc gatggcgctg
     2041 gcacagttgc gcggatataa actaccgtta cgcactgact ggctggatgc catagcaggc
     2101 tcgctgatta aagaagccct gaacgcgccg ttgccgtgga gctatcgcgg cgttattcat
     2161 cccgataccg atccgattct gctaacgttg atagacacat tagcgggtga cggattcggt
     2221 aaacttgccc cttctacgcc acaaccgcct ctgccaaaag atgtcacctg cgaactggaa
     2281 cgtaccgcaa tctctctttc ggcggagctt accttaaatc gctttaaccc caatggacta
     2341 gcgcaaagtc aggtgttaca tcggctggca atactggaga tcccagggat tgtacgccag
     2401 cagggaagca cactgacact tgcaggcaac ggtgaagaac gctggaaatt aacccgcccg
     2461 cttagccagc atgcggcatt gattgaggcc gcctgctttg gtgccacact ccaggaagcc
     2521 gcacgccata aattagaagc cgatatgctg gacgcgggtg gaatcggcag tatcaccaca
     2581 tgtcttagcc aggcggcgtt agcgggtctg gcgtccttca gtcaacaatt actggagcaa
     2641 ctcacattat taatcgccta ggaaaatcaa tttgccgaaa tgggcccggc gctggaagtg
     2701 ctatatgcct tatggcggct ggatgaaatt agcggtatgc aaggtgcgca gatatcacaa
     2761 acgacgttat gcgcggttat cgatcgcacg ctgtggctgt gtgaatctaa cggcagaccg
     2821 gatgaaaagg agtttcacgc tcacctgcat agctggcaag cgctttgcca tattctgcgc
     2881 gatctacata gcggcgttaa tttacccggc gtttctcttt ctgcggcggt agccttactg
     2941 gagcgacgca gtcaggcaat ccatgccccg gcgctggatc gcggcgcggc tcttggcgca
     3001 ctgatgcgtc tggaacatcc caacgctagt gccgaagcgg cgctgacgat gctggcgcag
     3061 ttatccccgg cacaatccgg cgaagcgctg cacggtttgc tggcgctggc ccgccatcaa
     3121 ctggcctgtc agccggcatt tatcgccggt ttcagcagtc atttaaatca accaagtgat
     3181 gccgatttta tcaatgccct gcccgattta cgcgcggcga tggcctggct accgccacga
     3241 gaacgcggga cactggcgca tcaggtgctt gaacattatc aactggcgca acttcccgtt
     3301 tcggcgctgc aaatgccgtt gcattgccca ccacaagcca ttgcacatca tcaacaactc
     3361 gaacagcagg cactggcatc gctgcaaaac tggggagttt tccatgtctg aactgaacga
     3421 tcttctgacc acccgtgagc tacaacgctg gcgattaatt cttggcgaag cggcagaaac
     3481 gacgctttgt gggctggatg acaacgcccg gcagatagac cacgcgctgg agtggctgta
     3541 tgggcgcgat cctgaacggc tccagcgtgg tgaacgctcc ggtggattag gtggctcaaa
     3601 tctcaccccc cctgagtgga tcaacagtat tcacacgctg tttccgcaac aggtgattga
     3661 gcggctggaa agcgatgccg tactgcgcta cggcattgaa gatgtggtga cgaatctcga
     3721 cgtgctggaa cgtatgcagc cttctgaaag cctgctacgc gccgttttgc acaccaaaca
     3781 tctgatgaac cccgaagtac tggctgccgc ccgccggata gtgcgccagg ttgttgaaga
     3841 aattatggct cgactggcaa aggaagttcg tcaggctttt tctggtgtcc gcgatcgccg
     3901 tcgccgctca tctattccac tggcgcgaga ctttgatttc aaaagtactc ttcgcgccaa
     3961 tctgcaacac tggcacccgc aacacggcaa gttgtatatc gaatcccccc gctttaacag
     4021 ccgaattaag cgccacagtg aacaatggca actggtctta ctggttgatc taagcggatc
     4081 gatggtcgat tcggtgatcc actctgcggt aatggcggcc tgtttgtggc agttacccgg
     4141 cattcgtacc catctggtgg cgtttgacac cagtgtcgtt gatctcacgg cagacgttgc
     4201 cgatccggta gagttattaa tgaaagtaca gttgggcggc gggaccaata tcgccagtgc
     4261 cgtggagtat gggcggcaac ttattgaaca accagcaaaa agcgtcatta tcctcgtgag
     4321 tgatttttat gaagggggtt catcatcatt gctgacgcat caggtgaaaa agtgtgtcca
     4381 gagcggcatc aaagtgctgg ggctggcagc gctcgacagc accgcaacgc cttgctatga
     4441 ccgcgatatg gcccaggcgc tggttaatgt tggcgcacaa atagccgcaa tgacaccggg
     4501 tgaactggct acctggcttg cggagaattt gcagtcatga attcactacg tccggaatta
     4561 ttagaactca cgccgcaggc cctgacggcg ttaagcaatg ccggttttgt taagcgcagc
     4621 cttaaggaac tggaaaatgg caacgtcccg gaaatcagcc atgagaacgg cgctttaatc
     4681 gccaccttca gtgacggtgt ccgtacccag ctggcgaacg gccaggcact gaaagaggct
     4741 cagtgcacct gcggggctag cggcatgtgc cgtcatcgcg tgatgctggt gttaagttat
     4801 cagcgacttt gtgccaccac ccggcccacg gaaaaagaag aagaatggca gcctgcaatc
     4861 tggctggaag aactggctac cctgcccgat gccacccgca aacgtgcaca ggtgctggtc
     4921 gctaaaggca tcaccattga gttgttctgt gcgcctggcg aaattccctc agcccgctta
     4981 ccgatgagtg atgtgcgttt ttattcccgc agcagcattc gcttcgcccg ctgtgattgt
     5041 attgaaggca cactttgcga acatgtcgta ctggctgtgc aggccttcgt cgaggccaaa
     5101 actcagcaag cggaatttac tcatttaatc tggcagatgc gcagcgaaca cgtcacatca
     5161 tctgacgacc cgtttgccag cgaagaaggc aacgcgtgtc gtcaatatgt tcagcaatta
     5221 agccaggcat tatggcttgg cggcatcagc cagccgctta ttcactacga ggccgctttc
     5281 agccgcgcgc agcaagcggc ggaacgctgc aactggcgct gggtgagtga atcgctacgg
     5341 caactgcgcg ccagcgttga tgccttccac gcccgcgcca gccactatca tgcaggagaa
     5401 tgcttacgtc agcttgcggc attaaacagt cgattaaatt gcgcacaaga gatggcccgg
     5461 cgcgacagtg ttggtgaagt tcctcctgtg ccgtggcgca cggtcgttgg ctctggcatt
     5521 gccggagaag caaagcttga tcatctgcgg ctggtgtctt taggtatgcg ttgctggcag
     5581 gatattgagc attatggttt acgcatctgg tttaccgatc ccgacaccgg cagtattttg
     5641 cacctttcgc gcagttggcc gcgaagtgaa caggaaaact caccggcagc tacgcgtcgg
     5701 ctgtttagtt ttcaggctgg cgcactggcg ggtgggcaaa ttgtttcaca agcagcaaaa
     5761 cgcagtgccg atggcgagct gctgttagct acccgcaacc gcttaagcag cgttgtgccg
     5821 ctgtcgcctg atgcctggcg aatgttgagc gcgccgttac gccagccggg cattgtggct
     5881 ttgcgggaat atttacgcca gcgtcccccc tcctgcatac ggcctcttaa tcaggtcgat
     5941 aacttattta ttctgccggt cgctgagtgt atttcgctcg gttgggatag cagccgccag
     6001 acgctggatg cgcaggtaat cagcggcgaa ggtgaagata atctgctgac gttatcactg
     6061 ccagcctcag ccagcgcgcc ttatgccgtt gaacgcatgg cggcgctttt gcaacaaaca
     6121 gacgaccccg tgtgtctggt ttctggcttt gtcagttttg ttgatgggca attgacactg
     6181 gaaccacagg taatgatgac aaagactcgt gcctgggcgc tggacgcaga aactgcgcct
     6241 gtggtggcat cactaccttc tgccagcgtt ctgcctgtgc cgtctactgc tcatcagttg
     6301 ctgatgcgct gccaggcatt acttattcaa ctgctccata acggctggcg ctatcaggaa
     6361 cagagtgcta ttggtcaggc agagttgctg gcgaatgacc tcaccgcggt gggtttttat
     6421 cggctggcac atgtattggg acaatttcgt aatacagaaa gcgaggcacg ggtagaagca
     6481 atgaataacg gtgttttgct gtgcgaacaa ttattccccc tgcttcagca acaaggatga
     6541 aatagtgctt tttactaaga gttctactcc agttccggac tgctcacgcc acggtattag
     6601 gcatatccta tatagcccct ggtgagagtc accagttcct tgattaaata aaatggagtt
     6661 ttacatgaag gctttcaata agctgttttc ccttgttgtt gcatctgttc tggttttttc
     6721 tcttgctggc tgcggtgaca aagaagaatc gaagaaattc agcgccaatc tgaacggcac
     6781 tgaaattgcc attacctatg tctacaaagg tgacaaggtg cttaagcaat cttctgaaac
     6841 caaaattcaa tttgcctcca ttggtgctac caccaaagaa gatgctgcca agacacttga
     6901 gccgttaagc gccaaataca aaaacatcgc gggtgttgaa gaaaaattaa cctataccga
     6961 tacctacgcg caggaaaacg tgactatcga tatggaaaaa gtggatttta aagccctgca
     7021 gggtatttca ggaatcaacg tttctgctga agatgccaaa aaaggtatca ctatggcgca
     7081 aatggaactg gtgatgaaag ccgctggttt taaagaagtg aaataatctg tcggcggcca
     7141 tgtttgcgca tggccgccat acccgtctta gcttttcttc acatgctggc gcgctgccag
     7201 tccacgcaga aaataacgta aaaattgatc gccgcattcg cggaagtttt tatgatccgg
     7261 tgcacgcatc atcgccgtaa tttccggcat cgaaacgcgg aactgctgtt cggtgaggat
     7321 cgccagaatg tcatcggttt tcagcgaaaa cgcgatgcgt aattttttca gcacgatgtt
     7381 gttattaatg cgacgttccg gctccagtgc cggagcagac tcatccttgc cgcgtttttc
     7441 ataaatcagg ccattgagga atgacgacaa aacaatgtcc ggacaacgct gaaaaccctc
     7501 ttcgtcttct ttacgtagcc agacggcgat ctgttccgcg gtggcttcga cattacccag
     7561 cgccagtata cgcaccaggt cattattatt ggctttcaaa atgtagcgca cgctgcgcag
     7621 aatatcgtta cttagcatga ggccttcagg tgttgatgag gcaaaaagcc attttagcag
     7681 tcttttacag gccaatcgcc tcttttaagc ttttcagata acggcggctg accggcacgg
     7741 ttaagccatt acgcaaaatc aactcggcct gaccgttatc ttccagacga atctcctgta
     7801 aatgcgcgag gttaaccaga tactgacgat ggcagcgcag tagtggtgta cgactttcca
     7861 gggtacgtaa tgtcaattcg gtaaagccct ctttcccttc gtggctggta acgtagacac
     7921 cgctcatccg actgctgaca aatgccacat ctttcatttg cagcaaataa atccgactat
     7981 gccccgtaca agggataaat ttcagcgcct gttgattttc cggtaacagc gaaacatcct
     8041 gcttgctgcg ctcctgacgc aatcgcgcca gcgttttctc cagtcgcgct tcatcaattg
     8101 gcttcagcag atagtcaaag gcatgttctt caaaggcttt aatagcgtat tcgtcaaacg
     8161 cggtgagaaa aacaatatac ggacgatgtt ccgggtcgag catccccacc atttccagac
     8221 cactgatgcg cggcatctgg atatcgagaa acagcacatc cgggcgcagt ttatgcaccg
     8281 cgccgatccc ttccacggcg tttgaacact caccaacgat ttcaatatcg ctctgctcct
     8341 gcaaaaatac gcgcaggttc tcccgtgcta acggttcatc atcgacaatt aagactttaa
     8401 tcatgcctcg tccctccatg gtagtcgtaa cgttattcgg gtgtaactat caggctcaca
     8461 ggcgacgctt atcccatagt catcgccaaa ccgttcacgt aaacgcttat ccaccagatt
     8521 catccccagc ccactggcat tggttaccgg ttgatacaaa ccggcattgt cttcgatctc
     8581 cagcatcaaa tgttgcccct cacgtcggac gctgattgcc actcgccctg tatccagcag
     8641 ttgtgatgtc ccatgtttaa tggcgttttc cactatcggt tgcagggtaa acgcgggcaa
     8701 ttgctgctgg gataattctt gcggaatagc aatgttgacc tgcaaccgcg actggaagcg
     8761 cgccttttca atttgcagat aagcattcac atgttcaatt tcgtcggcga gagtaacaaa
     8821 ctccgaaggc cgctttaagt ttttgcggaa aaaagtggaa agatactgca ccagctggct
     8881 ggcctgttcg ctgtcgcgac ggatcaccgc tttaatggtg ttaagcgcat taaacaaaaa
     8941 atgggggttc acctgggcgt gaagcagttt gatctctgac tgggtgagca tcgctttttg
     9001 ccgctcatat tgcccggcga ggatctgcgc cgaaagcagt tgcgcaatcc cctcgcccag
     9061 cgtgcggttg attgaactaa ataaacggtt tttggcttca tacaatttga tggtgcccat
     9121 cacccgctga ttttcaccac gcaacggaat taccagcgtc gaccccagtt tgcattgcgg
     9181 atgcaaagag caacgataag gtacttcgtt gccatcagcg tagaccactt caccggtttc
     9241 aatcgctttt aaagtataag ttgaagaaat cggtttgccg ggtaaatggt ggtcgtcacc
     9301 aattccggta aaggccagca atttctctcg atcggtaatc gcgactgcac caatatccag
     9361 ctccagatac agcacctgag ccactttcat gctgttcact tcgttaaacc cctggcgcaa
     9421 aatgccttcc gtcgaggctg ccactttcag cgcagtggca gaaaaagccg aagtgtattt
     9481 ttcaaacatc gcgcgtttat cgagcaatat acgcataaac agcgccgcgc cgacggtatt
     9541 ggtgaccatc attggcgcag caatattact caccagacgc accgcatctt cataaggtcg
     9601 ggcgatcgcc aggatgatca gcatttgcac catttcagcg acgaacgtga cggcaccagc
     9661 ggtaatgggg ttaaagactt tatcagtgcg cccgcgacgg atcaggatgc tgtgtaccag
     9721 gccgccgagc aatccttcaa cgatggtcga gatcatgcaa cttagcgcgg tcatgccccc
     9781 catcgaatat cgatgtaacc cgccggtcag gccaaccagc ccaccgacga ccggaccgcc
     9841 gagtaagccg cccattaccg cgcctatcgc acgggtattg gcaatagaat cgtcaatgtg
     9901 caacccaaac caggtgccca tgatgcagaa gatggaaaag acgatgtagc agagaaattt
     9961 atgcggcaga cgaaccgtga cctgcattaa cggtatgaat aatggcgttt tactcattaa
    10021 ccacgcaatg actaaaaaaa cgcacatctg ctgaagcagc agcaacacca gattaaaatc
    10081 gtacataccc gcaaaccaca catcccttta aaacgcgtaa catacattgc ctgcgtttaa
    10141 ctttctttga actcttgcag aaaaatgaga attcgtgagt acgatcactc aaaatcgcct
    10201 ggcaaaaata aaatcaccct ataaatgcac aaaaaacggg caaaactacc tggttcgcaa
    10261 aactgcgtct aaagttaaac cgggacctcg cgagcaaggg tgagacgatg gcgctttaca
    10321 caattggtga agtggcgttg ctttgtgata ttaaccctgt cacgttacgc gcgtgacgca
    10381 acatttttaa aaatcgacga agatcgtcca ggagcgccgt ttttcaaggg ttggatagat
    10441 gattcgttta ccatgtcgat gaacttcgac gaatttcgcc gataaaaagt gtcccataca
    10501 tgccccattg atgccccata aacaatatca ggaattgaca ctggttatcc atacagataa
    10561 aaataacacc tgtacataac tacagttaca ggagggtgtt atgcgtgttg agatctgtat
    10621 cgctaaagaa aaaatcacta aaatgccaaa cggtgctgtg gatgcgttaa aggaagaatt
    10681 aacccgacgc atcagtaaac gttatgacga tgtagaggtg atcgtaaaag ccaccagcaa
    10741 cgatggcctt tctgttacgc gcaccgccga taaagattca gctaaaactt ttgttcagga
    10801 gacgctgaaa gatacctggg agtctgctga cgagtggttt gttcgctaat aaacacgtaa
    10861 atcggtaacg gctggaaatc attcaatact cgcactatcg aaagttcacc agccaaccgc
    10921 gacacgctct tacatacgaa gtgccgcgct ttccttaata attttttagc agtactgtgt
    10981 aaataatgag cgacctaatc catgcatgac gcgtagtgcc tattgtgcat ctttctgcgt
    11041 ctctttttac tggacccaag ccaatgatgc tggcgatacc attcccgcca ggcccactta
    11101 caacagtaaa attcggagct ctgcctttac atacaataca atctacctaa caaatatccc
    11161 ccggacattg caa
//
LOCUS       DBFIFI010000142        11148 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.142, whole genome shotgun sequence.
ACCESSION   DBFIFI010000142 DBFIFI010000000
VERSION     DBFIFI010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11148
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.142"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(55..507)
                     /gene="gatA"
                     /locus_tag="N7B39_003465"
     CDS             complement(55..507)
                     /gene="gatA"
                     /locus_tag="N7B39_003465"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HGU2423382.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(517..1779)
                     /gene="gatZ"
                     /locus_tag="N7B39_003466"
     CDS             complement(517..1779)
                     /gene="gatZ"
                     /locus_tag="N7B39_003466"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HGU2423383.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFAIADKVGFARERIILGGDHLGPNCWQQENVDAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            complement(1808..2662)
                     /gene="gatY"
                     /locus_tag="N7B39_003467"
     CDS             complement(1808..2662)
                     /gene="gatY"
                     /locus_tag="N7B39_003467"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HGU2423384.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKHYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(2961..4013)
                     /gene="fbaB"
                     /locus_tag="N7B39_003468"
     CDS             complement(2961..4013)
                     /gene="fbaB"
                     /locus_tag="N7B39_003468"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HGU2423385.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            4270..5547
                     /gene="yegT"
                     /locus_tag="N7B39_003469"
     CDS             4270..5547
                     /gene="yegT"
                     /locus_tag="N7B39_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HGU2423386.1"
                     /translation="MKTTTKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFTGAALMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            5544..6548
                     /gene="yegU"
                     /locus_tag="N7B39_003470"
     CDS             5544..6548
                     /gene="yegU"
                     /locus_tag="N7B39_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HGU2423387.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPNLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFINDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRTTTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGINAIDPALKAELDAVNQLDFNRYATALAKYRQQREAA
                     "
     gene            6545..7510
                     /gene="yegV"
                     /locus_tag="N7B39_003471"
     CDS             6545..7510
                     /gene="yegV"
                     /locus_tag="N7B39_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HGU2423388.1"
                     /translation="MSGARLHTLLPELTTRQPVMVIGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPEALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFATPLIIRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(7484..8230)
                     /gene="yegW"
                     /locus_tag="N7B39_003472"
     CDS             complement(7484..8230)
                     /gene="yegW"
                     /locus_tag="N7B39_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGU2423389.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(8282..9100)
                     /gene="yegX"
                     /locus_tag="N7B39_003473"
     CDS             complement(8282..9100)
                     /gene="yegX"
                     /locus_tag="N7B39_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HGU2423390.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIHIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPVIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGSVDFNVFNGTEEELQAFVDGIKETP"
     gene            complement(9165..9965)
                     /gene="thiD"
                     /locus_tag="N7B39_003474"
     CDS             complement(9165..9965)
                     /gene="thiD"
                     /locus_tag="N7B39_003474"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HGU2423391.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRALLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(9962..10750)
                     /gene="thiM"
                     /locus_tag="N7B39_003475"
     CDS             complement(9962..10750)
                     /gene="thiM"
                     /locus_tag="N7B39_003475"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HGU2423392.1"
                     /translation="MQVDLLSSAQSAHTLHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGVANGGRGVDTTDAAVNAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDML
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(10785..10881)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            11084..>11148
                     /locus_tag="N7B39_003476"
     CDS             11084..>11148
                     /locus_tag="N7B39_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor"
                     /protein_id="HGU2423393.1"
                     /translation="MTETQIYENIKQAISSAPRNS"
BASE COUNT         2635 a         2893 c         2799 g         2821 t
ORIGIN      
        1 agcgacaata atcttgcgtt tcataaatag tcctttttat tgttacagag ggatttattc
       61 gtctgaatct aaaacatact ttgtgaagta ttcctttaac tgagtttcag gaagagtgat
      121 tagtgtctca acgatatcgg gctgttgtaa cttgccaaat aaacagcgta aaagtttcaa
      181 ttgctgctgc ggattttcca caatcaacgc aataaccaac gataccgcca cgtcattatc
      241 atcatccgct tgctgaaaat gaactttatt tgttggcctt aacagataaa tggctgacga
      301 cttagcatga atcgcctcac aatgcggtat tgcaatggcg tgctgctcaa gcattatccc
      361 ggtagggaat tctgcttctc tggcaattaa tgcctgtggc caggtatcat gaaccacacc
      421 tttggcgagc atctcattac cgatatgggt taaaacttcg ctacgatcga caaaagaaat
      481 tccgctacga acaaacaggt tagtcatata ccgtccttat tccgcacagc cgtagcgata
      541 ggcgcgcaaa acatcataaa ttttatccat aatcagctga tgcggtattg ctgataattc
      601 cccggactga atgcgttcaa attgtttggg aagatactga ctaatcatgc ccagtgggat
      661 gtccacgcct tgcagattca ccatcatcgt ttcaacgcta tttttaatcc gactatgcgg
      721 ccagtaataa cgaatacgat ccgacaggct gtaacgaata tccagtaatg aatcgttaaa
      781 acccgtgcga taatattttt tccagtattg cggttcgtcg agcatcactt cttcaattac
      841 cgccaggcaa ccgctgcgat tttcgggggc gataagttcc tgctcaattt gcgccagcgc
      901 aaatatcgcc tcacgtaaag caaaggttaa tgcgggaccg actttcaata ttgcaaagtg
      961 atcgcggact aattcccaat aagccgtccg ggtctggtaa tcggtagaat gtgcttcata
     1021 aaccattcgg gtattttcta tccattgcgc cagcgcctgc gcttcctgcg gctgataatg
     1081 gataatattg ctgtgatcaa attccacacc cggctgcacc acgatggcaa tcacgcgtgt
     1141 taatgcctct gtcagcccac gggcaataaa ggccttttga tgcgtacgta aagtattggc
     1201 ggcatcttca acatgggtga tgtgtactga ctgaatggcg ctggcctcac cgcccggaac
     1261 cggaacttcg gtgccaatga catagctcag ttgctcacgc tggcaatctg tcgccacact
     1321 ttccgcagca aagcaaagca cagcagctcg ttccgcaacc gtttctggtg ctaacggtat
     1381 gggatccccc gcgcaggaca ttgacgcatc aagatgaatt ttactgaagc cagcacgaac
     1441 atatgccttt accagctcga cggatttttc catcgccgca tccacatttt cttgctgcca
     1501 gcagtttggc cccagatggt caccgccgag aataatacgc tcgcgtgcaa atccgacttt
     1561 atcggcaatc gcaaaaacaa attcgcgaaa gtctgccggt gtcattccgg tataaccgcc
     1621 aaattgattg acctggtttg acgttgcttc aatcagcact ttgcgcgtgc tgttgcgatc
     1681 aaatgccagc gccgcttcga taaccaacgg atgggcagaa cagactgaac atatgccgat
     1741 atgttcacca gctttatgcc gggcaattaa cgttttcatg ttttttcctt gttaaatggc
     1801 aggtgcgtta tgccctgccc tcgcagccac aatcggcaat cactttgctc accacatcgc
     1861 gcattgcgga tttagccgac tgcaaataat cccggggatc ggtcgcttcg gggtgctcgg
     1921 tcaggtaatt ttttaacgcc tgcgagaagg catttttcag ctccgttgca acgttgattt
     1981 tgcatatccc cagtttgatg gtttgctgaa tatccttagt cgataaccct gacgcgccat
     2041 gcagcaccag cggtaagttc acccactggc gaatgttctc cagtcgagaa aaatcaagcg
     2101 ccggtgcgct ggcatacata ccatgagccg tgccgatggc gaccgccagg gaatcaattc
     2161 cggttgcctc ggcaaattca cgcgcctgag cggggttggt ataaaaagca tcggcttcat
     2221 tgacttgcac atcatcttcc tggccgccaa gttgccccag ctccgcttcg acgctgacat
     2281 caaagcgatg gcaaaaatcc accacctctt tgacccgtga aatattttgc gcaaaaggca
     2341 aatgcgaggc gtcaatcatg actgagcgca cgccagaacg aaccttctga gcgatatcgt
     2401 caaatttcgt gtgatggtcg agatgaatcg ccagtggatg gtgataatgc ttcgccatcg
     2461 cgctgaccag cgccaacaga ttttctgtac cagcatgagt aaatgtgcca ggcgttccgg
     2521 cgatgatgac cggcgcatgc aggttggcag cggtttctac caccacttgc atcgtttcga
     2581 gattgtgaat attgaatgcc ggaaccgcgt aaccgccgcg ctgcgcgttg ttcagcatct
     2641 gctttgtcga taccacgtac attttcacat cctgtcgatt gctttcgatt tcaaaacata
     2701 atgaaatcat ttgtttttaa atgtcgagat aacgatcaca aaaacgacaa tatgaaaatt
     2761 aatagaggaa tgaaaggcaa aaaaaacggc ctcccggagg gaagccgcaa caaagtgcgc
     2821 gtggttttat gcaggatgcg gcgtaaacat atgtggcagc gctgcgtagg catgataaga
     2881 cgcttcagcg tcgcatcagg cataggttgc cggatgcggc gtaaacgcct tatccgtcct
     2941 acggggtagt gccgttcgta tcaggcgata gtaattttgc tatcgagata aacgtcctgc
     3001 acggcgttaa tcagtttcac gccgtcagcc atcgatttct tgaacgcctt acgtccaaga
     3061 atcagcccca ttccgccagc gcgtttgttg ataaccgcag tacgcactgc atcactgagg
     3121 tcagtttcac cgcctgcagc accaccggag tttatcaacc cggcccggcc catatagcag
     3181 ttagctaact gataacgcac cagatcaatc gggttttcgc tggttaactt gctgtacacg
     3241 cgatcgtcgg tataaccgta attaattgct ttatagccgc cgttattttc cgccattttt
     3301 tgtttgacga tatctgcacc tatggttgcc gccagatggt tcgcctgacc agtcaggtcg
     3361 gcggagacat ggtaatcaac gccatctttc ttaaaggcgg agttacgcaa ataggcccac
     3421 agcactgtca ccatgcccag ctcgtgcgca cgttcaaaag ccgcagaaat ttcttcaatc
     3481 tggcgacgtg actcttccga gccaaaatag atagtcgcac caaccgccac cgcgcccatg
     3541 ttgaaggcct gctccacgct ggcatacagc gtttgatcgt aggtattcgg gtaacttagc
     3601 gtctcgttgt gattaagttt tacgagaaat ggaatgcgat gcgcatagcg ccgcgatacc
     3661 gacgccaaca cgccgtaagt tgatgccaca cagttacagc ccgcttcgat cgccagttca
     3721 acaatgtttt tcgggtcaaa gtagagcggg ttagcagcaa atgaagctcc ggcagagtgc
     3781 tcaacgccct ggtcaaccgg cagaatagaa agatatcctg tgccagccag acgcccggtg
     3841 ttgtacaacg tctgcatatt acgtaacacc gctggcgggc gattattgtc aatcatcacg
     3901 cggtctacgt agtcatgtcc ggggagataa agctggtcag aaggaatagt catacaacgg
     3961 tgctgtaaaa ggttgtcggc gtctttgcca agcaactgcg caatatctgt cattactatg
     4021 ctcccgtaaa ttccgattgg atatcggcta tggattgtcc tggcccgcct tttgcgggca
     4081 atcataatcc tggtcgttac gggcgagttt ttccatcttt tggatgtttt ttcagcgttt
     4141 tctgttggct cgattcatca gaaaaaatgt tagcgcggtc aagttttcac cgcaaaggta
     4201 tttaaaaggt attattaagt ggtattgtca tcgcgtacct tacattacct gtcatgaagg
     4261 aattaaaaga tgaaaacaac aacaaagctg tcgttcatga tgtttgttga atggtttatc
     4321 tggggcgcgt ggtttgtgcc attgtggctg tggttaagta aaagcggttt tagtgccgga
     4381 gaaattggct ggtcgtatgc ctgcacagcc attgcggcga tcctgtcgcc aattctggtt
     4441 ggctccatca ctgaccgctt tttctcggcg cagaaagtgc tggcggtatt gatgttcact
     4501 ggtgcggcgc tgatgtattt cgctgcgcaa cagaccactt ttgccgggtt cttcccgtta
     4561 ctgctggcct actcgctaac ctatatgccg accattgcgc tgactaacag catcgctttt
     4621 gccaacgtgc cggatgtgga gcgtgatttc ccgcgcattc gtgtgatggg cactatcggc
     4681 tggattgcct ctggtctggc atgtggtttc ttgccgcaaa tgctggggta tgccgatatc
     4741 tcaccgacta acatcccgct gctgattacc gccggaagtt ctgctctgct cggtgtgttt
     4801 gcgtttttcc tgcccgacac gccgccaaaa agcaccggca aaatggacat taaagtcatg
     4861 ctcggcctgg atgcgctgat cctgctgcgc gataaaaact tcctcgtctt tttcttctgt
     4921 tcattcctgt ttgcgatgcc actggcgttc tattacatct ttgccaacgg ttatctgacc
     4981 gaagttggca tgaaaaacgc caccggctgg atgacgctcg gccagttctc tgaaatattc
     5041 tttatgctgg cattgccgtt tttcaccaaa cgctttggta tcaaaaaggt attattgctt
     5101 ggtctggtca ccgctgcgat ccgctatggc ttctttattt acggtagtgc ggatgaatat
     5161 ttcacctacg cgttactgtt cctcggcatt ttgctgcacg gcgtaagtta cgatttttac
     5221 tacgttaccg cttacatcta tgtcgataaa aaagcccccg tgcatatgcg taccgctgcg
     5281 cagggactga tcacgctctg ctgccagggc ttcggcagtt tgctcggcta tcgtcttggc
     5341 ggtgtgatga tggaaaagat gttcgcttat caggaaccgg taaacggact gactttcaac
     5401 tggtccggga tgtggacttt tggcgcggtg atgattgcca ttatcgccgt gctgttcatg
     5461 atctttttcc gcgaatccga caacgaaatt acggctatca aggtcgatga tcgcgatatt
     5521 gcgttgacac aaggggaagt taaatgaaaa cagaacgtat tctcggtgct ctttatgggc
     5581 aggcgttagg ggatgcgatg gggatgccct ccgagctttg gccacgcagc cgcgttaaag
     5641 cacactttgg ctggattgac cgttttctgc ctggaccaaa ggagaacaac gcggcctgtt
     5701 attttaaccg cgccgaattc accgacgata cctcgatggc gctgtgtctg gcggatgcgt
     5761 tactggaacg tgaaggcaag atcgacccga atctgattgg gcgtaatatt ctcgactggg
     5821 cgctgcgttt cgacgccttt aacaaaaacg tactaggtcc gacctcgaag attgcgctta
     5881 acgccattcg cgacggtaaa cccgttgctg aactggaaaa caacggcgtg accaacggcg
     5941 cagcgatgcg cgtctcgcca ttaggttgtt tgcttccggc gcgtgatgtt gattccttta
     6001 ttaatgatgt ggcgctggcg tcgagcccga cccataaatc cgatctggcg gttgcgggcg
     6061 cggtagtcat cgcatgggcg atttctcgtg ctattgacgg agaaagctgg tcagcgattg
     6121 ttgattcatt gccttcaatt gcgcgacatg cacagcaaaa acgcaccacc acgttcagcg
     6181 cctcactggc agcgcggctg gagattgcgc tgaaaattgt gcgcaatgcc gacggcactg
     6241 aatccgccag cgaacagctt taccaggtcg ttggcgcagg taccagcact attgagtccg
     6301 ttccgtgcgc cattgcgctg gttgaactgg cacaaaccga cccgaatcgc tgcgccgtcc
     6361 tgtgcgctaa ccttggcggc gatacagaca ccatcggtgc tatggcgacg gcaatctgcg
     6421 gcgcgttgca tggcattaac gctatcgatc ctgcgttaaa ggcggagttg gatgcggtaa
     6481 atcagcttga tttcaaccgc tatgccacag cgctggcgaa atatcgtcaa caacgggagg
     6541 cggcatgagc ggcgctcgat tacacacgct gctgcctgaa ttaaccacgc gtcagccggt
     6601 tatggtgatt ggcgcggcag tcattgatgt gatcgccgac gcttatgccc tcccctggcg
     6661 tgggtgcgat atcgaactga aacagcagag cgttaacgtt ggcggctgcg cactgaatat
     6721 tgccgtggcg ttaaagcgcc tcggcatcga agcgggtaac gccttgccgc tcggtcaggg
     6781 cgtgtgggcg gagattattc gcaaccggat ggcaaaagag ggcttaatca gcctgatcga
     6841 taacgccgaa ggtgataacg gctggtgtct ggcgctggtt gagccggatg gcgaacgcac
     6901 ttttatgtca ttcagcggtg tggaaaatca gtggaatcgc cagtggctgg cgcgattaac
     6961 cgttgcgcct ggcagcctgc tctatttttc cggttatcaa ctggcctcgc cctgcggcga
     7021 attattagtg gaatggctgg aaaagctgca agacgtgacg ccgtttatcg attttggccc
     7081 acgtattggc gatatcccgg aggcattact ggcgcggatc atggcctgtc gacctttagt
     7141 gtcgctcaat cgtcaggagg ctgagattgc cgccgaacgt tttgctttat ccgcagagat
     7201 aacaacactt ggcaagcaat ggcaggagaa atttgccacg ccgttgatca ttcgtctcga
     7261 taaagagggc gcatggtatt tcagcaacga cgcttctggc tgcattccgg catttcccac
     7321 gcaagttgta gacaccattg gggcgggcga cagtcatgca ggtggcgtac ttgccgggtt
     7381 ggcctctggt ctgccactgg cggatgccgt attactgggc aatgcagtgg cgtcgtgggt
     7441 tgtcgggcat cggggcggtg attgtgcgcc aacgcgcgag gaactactcc tcgcacacaa
     7501 aaacgtatag atcgctgcga cagtggctaa tgctgtactc aataggccgt tgctgttggt
     7561 caagcgccac ttgcttgatc accagcaccg gtattttgct atctaactga atatgtgact
     7621 gaaactcggc atctggcatc cgggcgctaa cgcgggaacg tgtacgttgt gggtaaatgt
     7681 gctggctgcg gaaatagtca taaagagaaa ttccgatggc atcaacatca tgaattaaat
     7741 gcgccggaac ccacgattcc tcaatcgata ccgcttcttc atcgacatag cgaatacgct
     7801 taagcaaaaa gacatcactt cccgcttcga ccgccagctg ctgcgcgact tcttcgggac
     7861 atttcacgac acgcttgtta acccatagcg tatcgggctt tttcccgcgc aataccacct
     7921 gctgagaaaa acctcgcgct tctttcaacg agtattcgaa gatgttgttg atctgcgtgc
     7981 cgtaaccgcg cgaacgcgtc accacaccct cttcttccag cgcctgcatc gccttgcgca
     8041 ccgtaatgcg tgaaacgccg gttaactgac ttaaatcacg ctcaccgggc aaaatattgc
     8101 catgctccag caccccgctg cgcacggcat tttttaccgt ttcggcaaac ttaatataaa
     8161 gcggcgtgtt atccgccgct aaaatacgct cgttcagttg ggcaatcagc tgggtatgcg
     8221 cttgttccat ttgtcttttt cctgccaggg agtctggtgg gagtatatca ctgcttctta
     8281 tttaaggcgt ttctttaatc ccatcaacga atgcctgcaa ctcctcctct gtgccattaa
     8341 acacattaaa atccaccgaa ccattaatgc catctacctg cccacggtcg gaatgctgcc
     8401 agaaacgcca ggccatgccg tcattgtccg ggcgacgttg atagtagtga gccacccacc
     8461 acggatattc attgaaatag cccgccagat tggtgtgata aaaaacggct cctgagtaaa
     8521 taaccggctt ttttcccgta cttttttcga ccatttttag ccactgactt acccgcttgc
     8581 gtaattcttt tgccgataat tttccccgtt cttctacgtc cagcacggca gggaaatcgc
     8641 cttgtgagaa atccaccgtt tgcagaaata atcgcgcctg aacaggcgct gctaccgacg
     8701 gggagaaata atgatacgcc ccgcgcagca ggccattttc gcggcttagt tgccagttac
     8761 gcgaaaaata ggggtccacc agcttttcgc cttccgtcgc cttgataaag gcaaattgta
     8821 agcggatacc gttatcgcgc atttttgcca cccgctgcca gtcgatccgc tcctgccagc
     8881 gcgaaacatc tattccgtga atggtgtaac tggcaggaat atgaatagcg aaagatttaa
     8941 ccgggcgata accgtagaag tggatgtagt ctgtaatctg aactgctgtc gaatagaaaa
     9001 aattcaccgt ctgacgcgga taaattgcaa cgatggaaat tagcccaaga gcgcataata
     9061 gagcggtgag tttttttcga ctggtaattc ttagctgcat tggtttcatc cctgaatgtc
     9121 agtgccaaag gctgacaata accaaagcag ctatagtacg gtgctcacca ccaggcgtgg
     9181 aagtggtgaa ccggaccaat accgtgacca acttccagcg tgtcggcctg ggctaacgcc
     9241 gatgaaagcc agctttttgc ctcctgtacg gtgtcagccc agtttgtatg gcgcgggcgt
     9301 agtgcagcca acgccgcaga gagtgtacaa ccagtgccgt gggtgttttt ggtcataatg
     9361 cgcggtgcgg taaaccgttg ttcaccctcg cgggtaaaca gccagtccgg gctttgctca
     9421 tcatccagat ggccaccttt cattagcact gcgccacagc ccatcgccaa cagcgctcgc
     9481 ccttgttcca gcatttcctg ttcggtgcgc gcgtgtggcg cgtcgagcaa ggcagcagct
     9541 tcgggcaagt ttggcgttat taatgaaacc tgtggcaata atcgactgcg tagcgtggca
     9601 actgccgaag gtgaaagcag tgggtcgcca ctttttgcca gcataacggt gtcgagtacc
     9661 acgttttgga tctgataacg ttgcaaccgt tctgccaccg cttcaacaat atcggtttcc
     9721 gccaacatac cgattttagt ggtatcgatt cgcacatcgc tgaacaccga atcgagctgg
     9781 gcggcgacaa aatcaggctc aatgcgatac accgactgta cgccacgggt attttgcgcc
     9841 accagtgcag taataactga gcaaccataa gcgccaagtg ccgagaaggt tttaagatcg
     9901 gcctgaatcc ccgcaccacc actcggatca gtaccggcaa tcgtcagagc gttaattcgt
     9961 ttcatgcctg cacctcctgc gtcaattgcc agagcgcatc aaggaaatgt ggaacaaaac
    10021 tgcctggccc ctcgcttctg gcgactgcgc gttctccggc ttgtttcatc cagtgacagg
    10081 cagatgcgac attttccagc atatcgcctg gtaacgcaca acaggcagcg acaaccgccg
    10141 ataatgcaca gccagttcct accactttgg tcattaacgg atcgccaccg tgaatgccaa
    10201 cggcgcgatg gccatcggta acataatcca cctcgccagt gaccacgacg attgcgccag
    10261 tttcccgtgc cagtgtttgt gcagcgggta tcgcgttaac tgcggcgtca gtggtatcca
    10321 ctccccgtcc accattagca acgccagcta atgccatgat ttccgaagca ttaccacgta
    10381 tcgctgccgg tttaaaagat aaaagttcat gacaaaaatg gcggcgataa tcgagcgcac
    10441 caaccgctac tggatcaagc gtccagggtg tttgagagct ttttgcttgc tcaacggcag
    10501 cacgcatcgc ctgtgcgcgt ggctgcgtca gtgtgccaac gttaatcaac aaggcactgg
    10561 cgatagccgc aaactgactg gcctcttcgg tttcgataac catcgctggc gatgcaccga
    10621 gcgccagcaa ggtattggca gtaaaggttt gcaccacatc gttggtcatg cagtgcacaa
    10681 gaggggaatg ttggtgaaaa aggtgtaacg tgtgcgcaga ttgcgcggaa ctcagcaggt
    10741 cgacttgcat agtttgctcc tgccataacg tgaagaagca atgacctggt ggtccgtgac
    10801 ttccctacgc tggcattatc cagatcaggt gatacgggta tttctcagcc ttcacgcaga
    10861 agggcacccc gagtcgtttg gttgaatctc tatattttgt gccagcttag gggaagaggc
    10921 ttgcagatgc aagctcgcct ccattcatta cgtcttcact gtgtacccca tatcagtaat
    10981 gaaaaagaaa caattcacac ccatcattca ggtggtaaaa actgtagact atcttaatta
    11041 gctaatttat attatttatc taaatttgat aggtgagata aaaatgacag aaacccagat
    11101 ctacgagaac attaagcaag ccattagcag cgcaccacga aatagcca
//
LOCUS       DBFIFI010000143        11141 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.143, whole genome shotgun sequence.
ACCESSION   DBFIFI010000143 DBFIFI010000000
VERSION     DBFIFI010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11141
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.143"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(222..2051)
                     /gene="glmS"
                     /locus_tag="N7B39_003477"
     CDS             complement(222..2051)
                     /gene="glmS"
                     /locus_tag="N7B39_003477"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HGU2423394.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFAEQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2213..3583)
                     /gene="glmU"
                     /locus_tag="N7B39_003478"
     CDS             complement(2213..3583)
                     /gene="glmU"
                     /locus_tag="N7B39_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HGU2423395.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGSGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3935..4354)
                     /gene="atpC"
                     /locus_tag="N7B39_003479"
     CDS             complement(3935..4354)
                     /gene="atpC"
                     /locus_tag="N7B39_003479"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HGU2423396.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4375..5757)
                     /gene="atpD"
                     /locus_tag="N7B39_003480"
     CDS             complement(4375..5757)
                     /gene="atpD"
                     /locus_tag="N7B39_003480"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HGU2423397.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5784..6647)
                     /gene="atpG"
                     /locus_tag="N7B39_003481"
     CDS             complement(5784..6647)
                     /gene="atpG"
                     /locus_tag="N7B39_003481"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HGU2423398.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6698..8239)
                     /gene="atpA"
                     /locus_tag="N7B39_003482"
     CDS             complement(6698..8239)
                     /gene="atpA"
                     /locus_tag="N7B39_003482"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HGU2423399.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8252..8785)
                     /gene="atpH"
                     /locus_tag="N7B39_003483"
     CDS             complement(8252..8785)
                     /gene="atpH"
                     /locus_tag="N7B39_003483"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HGU2423400.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8800..9270)
                     /gene="atpF"
                     /locus_tag="N7B39_003484"
     CDS             complement(8800..9270)
                     /gene="atpF"
                     /locus_tag="N7B39_003484"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HGU2423401.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9332..9571)
                     /gene="atpE"
                     /locus_tag="N7B39_003485"
     CDS             complement(9332..9571)
                     /gene="atpE"
                     /locus_tag="N7B39_003485"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HGU2423402.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9618..10433)
                     /gene="atpB"
                     /locus_tag="N7B39_003486"
     CDS             complement(9618..10433)
                     /gene="atpB"
                     /locus_tag="N7B39_003486"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HGU2423403.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10442..10822)
                     /gene="atpI"
                     /locus_tag="N7B39_003487"
     CDS             complement(10442..10822)
                     /gene="atpI"
                     /locus_tag="N7B39_003487"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HGU2423404.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
BASE COUNT         2673 a         3029 c         2760 g         2679 t
ORIGIN      
        1 tcttgaatga acaaaacgtg ctattcatta caaagtacat caattatggc aattgtttgt
       61 caatgattcg aggtatttaa ttactactac ggttaaaaca agtgttcaac ggtgccccaa
      121 ataatagctg aagttaatta tatggaatga ggttagatta ttaatgctgg taagtaaaag
      181 ggccgccaaa aggcagccct tcgttcagca aatgaataat attactcaac cgttaccgat
      241 tttgccaggt tacgcggctg gtcaacgtcg gtgcctttga tcagcgcgac gtgataagcc
      301 agtaactgca gcggaacggt gtagaagatc ggtgcaatca cctcttccac atgcggcatc
      361 tcgatgatgt gcatgttatc gctacttaca aaacccgcat cctgctcggc gaaaacatac
      421 aattggccgc cacgcgcgcg aacttcttca atgttggatt tcagtttttc cagcaattcg
      481 ttgttcggcg caacgacgat aaccggcata tcggcgtcaa tcagcgccag cggaccgtgt
      541 ttcagttcgc ctgcagcgta ggcttcagcg tgaatataag agatctcttt cagcttcaat
      601 gcgccttcca gcgcgattgg gtactgatcg ccacggccca ggaacagcgc gtgatgtttg
      661 tcagagaaat cttctgccag agcttcaatg cgtttgtcct gagacagcat ctgctcaata
      721 cggctcggca gcgcctgcag accatgcaca atgtcatgtt caatggaggc atccagacct
      781 ttcaggcgag acagcttcgc caccagcatc aacagcacag ttaactgagt ggtgaatgct
      841 ttagtggatg ccacgccgat ttccgtaccc gcgttggtca tcagcgccag atcggattcg
      901 cgcaccagag aagaacccgg aacgttacag attgccagcg aaccaaggta acccagctct
      961 ttcgacagac gcaggcccgc cagggtatcc gcggtttcgc cagactgtga caaggtgatc
     1021 atcaggctgt tacgacgcac ggcagattta cgatagcgga attcagaggc gatttcgacg
     1081 tcgcacggaa tacctgccag cgattcaaac cagtaacggg aaaccatacc ggagttataa
     1141 gaagtaccac aggcgaggat ctgaatatgc tcaaccttcg acagcagttc gtcggcgttc
     1201 ggtcccagct cgcttaaatc aacctgaccg tggctgatgc gcccggtaag ggtgtttttg
     1261 atcgcgttcg gctgttcgta gatctctttc tgcatgtagt gacggtaaat acctttatcg
     1321 cccgcgtcat attgcagatt ggattcgata tcctgacgtt ttacttccgc gccagtttta
     1381 tcgaagatgt ttaccgaacg gcgagtgatt tccgcaatat cgccctcttc aaggaagata
     1441 aagcgacggg tcaccggcag cagcgccagc tggtcagaag cgataaagtt ttcacccata
     1501 cccagaccaa tcaccagcgg actaccagaa cgtgctgcca gcagggtatc cgggtgacgg
     1561 gagtccatga tcactgtacc gtacgcacca cgcagttgcg ggatagcacg cagaacagcc
     1621 tcacgcagag taccgccctg tttcagctcc cagttcacca gatgggcaat cacttcggtg
     1681 tcggtttcag aaacgaaggt atagccacgc gcttttaact cttcgcgcag cggttcatgg
     1741 ttttcgatga tgccgttatg caccaccaca atgtgttcag aaacatgcgg atgcgcattc
     1801 acttctgaag gttcaccgtg ggtcgcccag cgagtgtgag caataccggt gccgccatgc
     1861 agaggatgtt cttccgctgc ctgagccagc atctggactt taccgaggcg acgcaggcgg
     1921 gtcatatgac cttccgcatc aacaacggcc agaccggcag agtcatatcc gcggtattcc
     1981 agacgacgta aaccttcaag aaggatttct gctacatcac gttgcgcgat cgcgccaaca
     2041 attccacaca tagtttttga ttccgattta tatcgttgtc ggtcaacctg tatgcccgtt
     2101 ttttcgggcg ccccgagcct tgtagagagt ggggttattt ttatagttac tgcttgtggg
     2161 agggagatta tgttatctcc tcatcccatg tgaccggatt acccggccag aatcactttt
     2221 tctttaccgg acgacgccag ccttctttct gagtctgcgg cacacggctg atagctaatg
     2281 cattttcgcc gacattacgc gtcacagttg tacccgcagc aatggtcgcg cctttgccta
     2341 ctgttaccgg ggccaccagc tgagtgtcgg aaccgacaaa cacatcgtcg ccgataatgg
     2401 tcttaaattt attcgcacca tcgtagttgc aggtaatggt tcccgcgccg atgttaacgt
     2461 tatcgccaat ttccgcatcg ccaaggtaag tcagatgacc agctttcgag cctttaccca
     2521 gacgtgcttt tttcatctca acaaagttac cgacgtgtgc accttccaac aactcagcac
     2581 caggacgcaa acgggcaaac gggccaatgg tacaggccgc cgccagattc gcgtcttcca
     2641 cgacggtata tgggctaatt tcgcaatcat cgccaatcac gctgttttta atcacgcaac
     2701 cggagccgat tttcacgcga tggccgagag tcacgttgcc ctcgatgata acgttagtat
     2761 caatttcaac atcgcgcccg tgagtaagcg taccgcgcag atcaaaacgc gccggatcgc
     2821 gcagcataac gcctgctaac agcagttttt cagcctgttc ggactggtaa acgcgctcca
     2881 gacgggagag ttgcaggcgg ttattcacgc cttctacttc gcttaaacgt tgcggatgaa
     2941 cggcgacgat ttcacgccct tcctgatacg ccagcgcaat aatgtcggtg atgtagtatt
     3001 cgccctgcgc attattgttg gtcagcttcg ccagccagcg tttcatatct gcgccgttag
     3061 cgatcagaat gccggtgttg atctcctgaa tctgacgctg ctcgtcggtg gcatctttgt
     3121 gctcaacaat gccggtaact ttgccgtttt cacgggtgat acgtccataa ccggtcggat
     3181 catccagttt caccgtcagc agaccaatgc caccctgcgg tttagcatca cgcagacgct
     3241 ggagtgtttc gacagagatc agcggcacgt cgccgtagag cattaaaatg tcttcatcat
     3301 cggcaaagaa aggtgcggcc tgctgcatgg catgacccgt acccagctgc tctgcctgaa
     3361 gcacccagtt caggttgtcg tctttcagcg cctgttttag cagatcgcca ccgtgaccgt
     3421 acaccaggtg aacgtgcgct gcgcctaatt cattcgcagc atcaatgaca tgctgaacca
     3481 tcgctttccc ggcaagggta tgcagcactt tcggaagatc ggaatacatg cgcgtgcctt
     3541 tacctgcggc aaggatcact acgctcatag cattattcaa catacgcgtc ctgactgtaa
     3601 tttgagaacg aatttaaacc gcttcacctt gaaaaaacta catttttttc atcgtgaaat
     3661 ggacagagga taaattgttc aataacggat tatccccgct gacgaaagcg ccattttcga
     3721 ccatcgtcac gccttttgtc tgcagaaaat taaggcagta ccttcattta agcgtcaggt
     3781 ggatgttttt gctcttattt cgatcaatga ataaacggga aaacaggggg ttttatatca
     3841 gcaggatcta tgtgaacgct attcaggacg ggtcacacgc gcaaaaaaaa gccagcctgt
     3901 ttccagactg gcttttgtgc ttttcaagcc ggtgttacat cgcttttttg gtcaactcga
     3961 taacgcgcag ctgcgcgatc gctttggcca gttccgcaga cgcctgagcg taatctacgt
     4021 cgccgtgaga gctgctaatg tgctcttcag ccttacgttt cgcttccatg gctcgcgctt
     4081 cgtcgagatc ctggccgcga attgcggtgt cggccagaac ggtcacgttg ccaggctgca
     4141 cttcaagaat gccgccagac agatagataa actcttcgtg accgtgctgt ttcacgatgc
     4201 gaatcatacc aggcttaatg gcggtgagca gcggtgcgtg gccagggtag atccccagtt
     4261 caccttcgct acccgttacc tggattttct cgaccagacc agagaacatt tgttgctctg
     4321 cgctgacgac gtccaggtgg taagtcattg ccatatcacc ctccgattaa ggcgttaaag
     4381 ttttttggct ttttccacag cttcttcgat ggaaccgacc atgtagaacg cctgctccgg
     4441 caggtgatcg tattcgcctt ccatgatgcc tttaaagcca cggatggtgt ctttcaggga
     4501 gacgtattta cccggagaac cggtgaatac ttctgccacg aagaacggct gggacaggaa
     4561 gcgctggatc ttacgagcac gcgctaccac cagtttgtct tcttcagaca gttcatccat
     4621 acccaggatg gcgatgatgt ctttcagttc ctgataacgt tgcaggatgg actgaacgcc
     4681 acgcgcggtg tcgtagtgtt cctgaccaac caccagcggg tccagctgac ggctggtgga
     4741 gtccagcggg tcaacggccg ggtagatacc cagagacgcg atctgacggc tcagtaccac
     4801 ggttgcgtca aggtgcgcaa aggtggttgc cggagacggg tcagtcaagt catccgcagg
     4861 tacgtatact gcctgtacgg aggtgataga accggttttg gtggaggtga tacgttcctg
     4921 cagaacgccc atctcttccg ccagggtcgg ctgataacct accgctgaag gcatacggcc
     4981 cagcagtgcg gatacttccg taccggccag ggtgtaacga tagatgttgt caacgaacag
     5041 cagaacgtca cgaccttcgt cacggaattt ctcagccatg gtcagaccgg tcagagctac
     5101 gcgcagacgg tttcccggcg gctcgttcat ctggccatac accagggata ctttatcgat
     5161 aacgttggag tcggtcattt cgtggtagaa gtcgttcccc tcacgagtac gttcacctac
     5221 gcccgcaaac acagagtaac cggagtgctc gatcgcgatg ttacgaatga gctccatcat
     5281 gtttacggtt ttacctacac ccgcaccacc gaacagacca actttaccgc ccttagcgaa
     5341 cggacacatc aggtcgataa ctttgatacc ggtttccagc agttcctgag agtttgacag
     5401 ctcttcgtag gaaggtgctg cgcggtgaat cgcccaacgc tcttcttcac cgatctcgcc
     5461 tttcatgtcg accggttcac ccagtacgtt catgatacgg cccagagtcg ctttacctac
     5521 cgggacttcg atcgggtgtt cgaggtcttt tacatccaga ccgcgacgca gaccgtcgga
     5581 ggaacccatt gcgatggtac gcacgatacc gccgccgagc tgctgctgaa cttccagcac
     5641 cagacgctca ttaccatttt gcacctcaag agcatcgtac acgcgcggta cggcatcctg
     5701 agggaattcg acgtcaacta cggcgccgat tacctggaca atctttccag tagccatctt
     5761 aaatcctcta cgaaataacc tgtttaaacc gcggcggccc ccgagacgat ctcggtgagt
     5821 tcctgagtaa tgctggcctg acgagctttg ttgtatacca actgcagctc tttaatcagg
     5881 ctgccgccat tgtcggtcgc ggctttcatc gccaccatac gggcggcctg ctcgctggcc
     5941 aggttttcaa ccacgccctg ataaacctga gattcgacat aacgacgcag cagggtatcc
     6001 agcaacgcct tcggatcggg ttcgtacagg taatcccagg atttatgttt cagatcatca
     6061 tcatctgatg ccggtaacgg cagcagctgg ctgatggtcg gaacctgaga catggtgtta
     6121 ataaatttgt tgctgacaat gtaaagcttg tccagacggc cttcgtcgta ggcctgcaac
     6181 atcactttta ccggaccgat cagttcggac agggaagggt tatcccccat gccggtgacc
     6241 tgggcaacaa cattgccgcc cacggagttg aagaacgaca cgcctttcga gccgatcatt
     6301 gcgaggtcgc attgaacgcc tttgtcggtc caggtcttca tttccgccag cagttttttg
     6361 aacaggttaa tgttcaaacc accgcacaaa ccacggtcgg tcgacaccac caggtagccc
     6421 acgcgtttaa cgtcgcggtc ttccaggtaa gggtgcttat attccagatt accgtgtgca
     6481 aggtgaccaa tcactttgcg catggtttct gcataaggac ggctggccgc catgcgatcc
     6541 tgcgatttac gcattttgga agcggcgacc atctccatcg ctttagtgat cttttgcgtg
     6601 ttctggacgc ttgcgatctt actacgtatc tcttttgcgc cggccatgag cttctcctca
     6661 atgccttgcg gcctgcccta aggcaagccg ccagacgtta ccaggattgg gttgctttga
     6721 aggaatcgag gatgcctttc agcttgcctt cgatttcgtc gttgtagcca ccggtctggt
     6781 tgatctcttg catcaacgga gcgtgatcac ggtcgacgta agccagcaga gcggcttcga
     6841 agctgccgat tttcgacagt tcaacatccg ccaggtaacc acgttctgct gcgaacagaa
     6901 ccagagactg ctgcgcaacg gacatcggcg catactgttt ctgtttcagc agttcggtca
     6961 ctttttgacc gtggtcaagc tgcttacgtg ttgcatcgtc aaggtcggat gcaaactgag
     7021 agaacgctgc cagttcacga tactgtgcca gagcggtacg gataccaccg gacagttttt
     7081 tcatgatctt ggtctgtgct gcaccaccaa cacgggatac ggaaataccc gggttaaccg
     7141 caggacgaat accggcgttg aacaggttgg tttccaggaa gatctgacca tcggtaatgg
     7201 agattacgtt ggtcggaacg aacgcagaaa cgtcacccgc ttgagtttcg ataatcggca
     7261 gcgcggtcag tgaaccggtt ttccctttca cttcaccttt ggtgaaagct tcaacgtatt
     7321 cggcgttaac acgcgcagca cgctccagca gacgagagtg gaggtagaat acgtcgcccg
     7381 ggaatgcttc acgtcctggc ggacgacgga gcagcagcga gatctgacgg taagcaacag
     7441 cctgtttaga cagatcatcg taaatgatca gcgcatcttc accgcggtca cggaagtatt
     7501 cgcccattgc gcaaccggca tacggtgcca ggtattgcag tgcagcggat tcagacgcgg
     7561 ttgctaccac aacgatggtg ttagccagtg cgccgtgctc ttccagttta cgtaccacgt
     7621 tagaaatggt ggacgctttc tggccgatag cgacatagat acatttgata ccggaatcgc
     7681 gctggttgat gatggcatcg atagccagtg cggttttacc ggtctgacgg tcaccgatga
     7741 tcaattcacg ctgaccacga ccgattggga tcatggagtc aacggcttta taaccggtct
     7801 gtaccggctg atctacggac tgacgttcga taacgcccgg agcgattgct tctacagcag
     7861 agaagccgtc gtgatccagc ggacctttac cgtcgattgg tgcacccaga gtgttaacca
     7921 cacggcccag caggccacgg ccaaccggaa cttccaggat acggccagta cacttaactt
     7981 tcatgccttc ggcaaggtca gcatacggac ccataacaac cgcaccaaca gagtcgcgct
     8041 cgaggttcag tgcgatagcg taacggttac ccggcaggga gatcatttca ccctgcatac
     8101 aatcggccag gccgtgaatg cggataacac cgtcacttac agaaacaata gtaccttcgt
     8161 tgtgagcttc actcacaaca ttgaactgag caatgcgctg cttgatcagt tcgctgattt
     8221 cggtggaatt cagttgcatg ctccagtccc cttaagactg caagacgtct gcaaggcgct
     8281 caagacgacc gcgtacgctg ccatcaatga ccatatcacc cgctcggatg ataacgcctg
     8341 ccattacaga cttatcgatt ttgcaattca gcttaacttt gcgtgacaga cgtttttcca
     8401 tcgcagcaga aattttcgcg agctgttgtt cactcagtgc ggcagcggaa atgacgtcta
     8461 cctcagcggt agcctcactc acggcacgaa ggtgaataaa ctgctccaaa acatccggga
     8521 gcgcgttaag acgaccattt tcagccatta cccgaataag gttctgaccg ttttcgtcca
     8581 gttgctcacc acaaactgcg ataaacgact cggcaagcgt ttctggcgca agcgcgccag
     8641 agagaagctc tgccatttgt tcgtttttgg ttacctcggc ggcaaacgcc agcatgtcct
     8701 gccagcgttc tacactttgg tgttcgacgg caaagtcaaa agctgctttg gcgtaggggc
     8761 gagctaccgt aataaattca gacatcagcc cctccctcct tacagttcag cgacaagttt
     8821 atccacgatg tcgctgttag cagcttcatc cacggaacgt tcgatgatct tctcggcgcc
     8881 agcaacagcc aggatagcaa cttgcttacg cagctcttca cgggcacgtt tacgctcggc
     8941 ttcaatttcc gcctgcgcct gggccacgat tttagtacgt tcctgttctg cctcagcttt
     9001 cgcttcgtcc agaatctgcg agcggcgttt gttcgcctgc tcgatgatta cctgggcttc
     9061 cgctttcgct tttttcagct ggtcggtcgc gctggccttt gcaaggtcaa ggtccttatg
     9121 tgctcgttct gcggaagcaa ggccgtcagc aatttctttt tgacgttttt cgatggctgc
     9181 cattaatggc ggccatacgt acttcatgca gaacagaacg aacaggacaa acgcgatggc
     9241 ctggccgagg attgttgcgt taagattcac agcacaatgc ctctatttag ttaacgttct
     9301 gatattgctc tttaaataaa agcaacgctt actacgcgac agcgaacatc acgtacagac
     9361 ccagacctac agcgatcatc gggatagcat ccaccagacc cataacgata aagaactgag
     9421 tacgcagcag aggaatcaga tcaggttgac gcgctgcgcc ttccaggaat ttacccccga
     9481 ggatgccgat accgatcgca gcaccgattg ccgccagacc catcatcaca gcggcagcca
     9541 tgtacagcag atccatattc aggttttcca tgacagtctc cagtttgttt cagttaaaac
     9601 gtagtagtgt tggtaaatta atgttcttca gacgccatcg acagatagac gatcgtcaga
     9661 accatgaaga tgaaggcttg cagcgtaatg atcaggatgt ggaaaatggc ccacggcaca
     9721 ttcaggatcc actgtgacca ccacggcaac agaccagcaa tcagaatgaa aatcagctca
     9781 ccggcataca tgttaccgaa cagtcgcaaa ccgagtgaaa ctggtttgga cagcaggctt
     9841 accccttcaa ggattaagtt gacaggaatg aacgcccagt gattgaacgg ctgcagcgtc
     9901 aactctttcg tgaagccgcc gatgcctttc attttgatgc tgtagaacag aatcaggata
     9961 aatacgccca gtgccataga cagcgttacg ttcacgtccg cagacggaac cacacgcagt
    10021 gcaggcagac ccagtacatg ttcagcaatg tacggcagca ggtcgatagg cagtaaatcc
    10081 atcaggttca tcaggaatac ccagacgaag atcgtcaggg ccagcggagc aatcagcttg
    10141 cttttgccat ggtacatgtc tttcacgcta ccattaacaa agccgatcac cagctcaatc
    10201 gcggtctgaa acttacctgg cacaccgctg gtcgcctttt tggctacgct acggaataaa
    10261 accaggaaca acagacccag caccaccgag aagaacatgg agtcaatatt gattgtccag
    10321 aaggtggctg gggggttttg tggatccacc agcgagaatg tacgcaggtc cagctgaagg
    10381 ttattcaggt ggtgtcctat gtaatcctgc ggcgtcatat tttctgaagc catgatgcct
    10441 tttacccttt gttgttaatt acagccggtg ccagtatctg aaccaccagc accaaaaccc
    10501 acgtaacgat cagcggcaag aataccgcct ttaaaaccgc caacgccacc accagtaaca
    10561 ccaacatcgc cagaactttg aaagcttcac caaatgcgaa tgtccaggcc acccggcctt
    10621 tcgctggtgt atgcgcctgg tgacgccagg caaatatcat aaacaaaacg ttaggcagaa
    10681 agactgccag gcccccgctt attgcagaga cgccccagaa ggggtctttg aggctgaaca
    10741 gcaatccact tgctatcacc accagtaact gaacgagcag aagcttccga gcaacgtttc
    10801 gactcacgag cgacacagac atcacgtttt tcactcctgc tcccttcgag gtatgccgcg
    10861 tgtcgtataa aactttcttt aaggcttaga gtcaagcatc aaaaagcggt caaattatac
    10921 ggtgcgcccc cgtgatttca aacaataagt agccaaaagg tgaataaatg tttaaatatt
    10981 tttccagtgc atacaattgc gacttttcta ctaaccctgt tcgatcatga aaaactgtaa
    11041 ataacgcgta aacactggtg ataaagcgtg cttcagatca catattgcgc atgttcgcgc
    11101 acagcatatt tatttacttg gcaaatgatg cctttgcaag t
//
LOCUS       DBFIFI010000144        10955 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.144, whole genome shotgun sequence.
ACCESSION   DBFIFI010000144 DBFIFI010000000
VERSION     DBFIFI010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10955
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.144"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            51..812
                     /gene="adiY"
                     /locus_tag="N7B39_003488"
     CDS             51..812
                     /gene="adiY"
                     /locus_tag="N7B39_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HGU2423405.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            949..2286
                     /gene="adiC"
                     /locus_tag="N7B39_003489"
     CDS             949..2286
                     /gene="adiC"
                     /locus_tag="N7B39_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HGU2423406.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2390..4033
                     /gene="eptA"
                     /locus_tag="N7B39_003490"
     CDS             2390..4033
                     /gene="eptA"
                     /locus_tag="N7B39_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HGU2423407.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            4030..4698
                     /gene="basR"
                     /locus_tag="N7B39_003491"
     CDS             4030..4698
                     /gene="basR"
                     /locus_tag="N7B39_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HGU2423408.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4699..5799
                     /gene="pmrB"
                     /locus_tag="N7B39_003492"
     CDS             4699..5799
                     /gene="pmrB"
                     /locus_tag="N7B39_003492"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HGU2423409.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(5775..5864)
                     /gene="pmrR"
                     /locus_tag="N7B39_003493"
     CDS             complement(5775..5864)
                     /gene="pmrR"
                     /locus_tag="N7B39_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HGU2423410.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(5976..7478)
                     /gene="proP"
                     /locus_tag="N7B39_003494"
     CDS             complement(5976..7478)
                     /gene="proP"
                     /locus_tag="N7B39_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HGU2423411.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVVGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7742..8620)
                     /gene="rdcB"
                     /locus_tag="N7B39_003495"
     CDS             complement(7742..8620)
                     /gene="rdcB"
                     /locus_tag="N7B39_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HGU2423412.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLARVQLQTWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGEQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMLEEIFSVNLV"
     gene            complement(8617..10845)
                     /gene="crfC"
                     /locus_tag="N7B39_003496"
     CDS             complement(8617..10845)
                     /gene="crfC"
                     /locus_tag="N7B39_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HGU2423413.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQKLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPPPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQMLLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQIEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALKVQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT         2739 a         2861 c         2698 g         2657 t
ORIGIN      
        1 gctttgcttt agcgagaact ggtcttttat tcgcactcag gagtacatgt atgaggattt
       61 gcagcgacca accttgtatt gttttattga ctgaaaaaga tgtctggata agggttaatg
      121 ggaaagaacc tattagcctt aaagctaacc atatggcgtt attaaattgt gaaaataata
      181 ttatcgacgt ctcctctctt aacaacactt tggttgctca tattagtcac gacatcatca
      241 aagattacct ccggtttctg aataaagatc tctcgcaaat accagtatgg caacgtagcg
      301 ctacgcccat tctcaccctg ccatgcctga caccagacgt ctttcgcgtt gccgcgcaac
      361 acagcatgat gcccgcagaa actgagtcag aaaaggaacg aacacgcgca ttattattca
      421 cggtgctatc ccgttttctc gacagtaaaa aattcctttc attaatgatg tatatgttac
      481 gtaattgtgt aagtgacagc gtttatcaaa ttattgaaag cgatattcac aaagactgga
      541 atcttagtat ggtagccagt tgtttatgtc ttagcccaag tctgttaaag aaaaagctga
      601 aaagcgaaaa caccagttat agccaaataa tcaccacctg ccgcatgcgt tatgccgtaa
      661 atgaattaat gatggacggt aaaaatatct ctcaggtatc acagtcctgc ggctacaaca
      721 gtacgtcgta ctttatttct gtctttaaag acttctacgg tatgacaccg ctgcattatg
      781 ttagtcagca cagagaacgc actgtcgcct gatttttaac cttaacgaag agctatatta
      841 ataacggcat cagcgataac ccggtcgata ataattcaac tatcgaatgc aggcgtatga
      901 tatgacgtaa tttattgtca cgaagctcgc cttcgcagga gtttaattat gtcttcggat
      961 gctgatgctc acaaagtggg cttaatcccc gtcaccctga tggtgtcggg gaatattatg
     1021 gggtcaggtg tttttctgtt acctgcaaac ctggcctcca ccggcgggat tgccatttat
     1081 ggatggttgg tgacgattat cggtgcgctg gggctctcga tggtatacgc caaaatgtcg
     1141 ttcctcgacc caagtcctgg tggttcttac gcttacgccc gccgctgctt tggcccgttt
     1201 ctcggttatc aaaccaacgt cctctactgg ctggcctgct ggatcggcaa tatcgccatg
     1261 gtggtcattg gcgtaggata tttaagttac ttcttcccga ttctgaaaga tccattggta
     1321 ttaaccatca cctgcgtcgt ggtgctgtgg atcttcgtcc tgctgaacat tgtcggtccg
     1381 aaaatgatca cccgtgtgca ggccgttgcc accgtgctgg cgctgatccc catcgtcggg
     1441 attgccgtat ttggctggtt ctggtttcgt ggtgaaacct atatggcggc atggaacgtc
     1501 agcggcctgg gcaccttcgg tgcaattcaa agtaccctta acgttacgct gtggtcgttc
     1561 atcggtgtgg aaagtgcctc cgttgccgca ggtgtggtga aaaacccgaa acgcaacgtc
     1621 cctatagcca ccattggtgg ggtattgatt gccgccgttt gctatgtgct ctccaccacc
     1681 gcgattatgg ggatgatccc taatgccgca ctgcgcgttt ctgcctcgcc attcggtgat
     1741 gccgcacgga tggcgttggg tgacaccgcc ggggccatcg tttccttctg cgcagctgcg
     1801 ggttgcttag gttcactggg cggctggaca ttgctggcgg gtcaaacggc gaaagcggct
     1861 gccgatgacg gactgttccc accgattttt gcccgtgtaa ataaagcggg tacgccagtg
     1921 gcggggctga ttatcgtcgg tattttgatg accatcttcc agctcagcag catttcacca
     1981 aacgcgacca aagaattcgg tctggtttct tccgtgtcgg tcatctttac actggtgcca
     2041 tatctttaca cctgtgcggc gttactgctg ctcggacacg gtcactttgg taaagcacgc
     2101 ccggcatatc tggcggttac taccattgcc ttcctctact gcatctgggc cgtggtgggg
     2161 tccggggcga aagaggttat gtggtcattt gtcaccctga tggtcatcac cgccatgtat
     2221 gcgctgaatt acaaccggct gcataaaaac ccgtatccct tagatgcacc aataagcaaa
     2281 gattaattcc ccttaatcca gcaaagataa aagccaacct taagaactta aggttggctt
     2341 aattttgctt tgcgagcata tgcgcacttt gttcgatgga aacaccgtga tgttgaagcg
     2401 cctactaaaa agaccctctt tgaatttact cgcctggcta ttgttggccg ctttttatat
     2461 ctctatctgc ctgaatattg ccttttttaa acaggtgttg caggcgctgc cgctggactc
     2521 gctgcataac gtactggttt tcttgtcgat gccggtcgta gccttcagcg tgattaatat
     2581 tgtcctgaca ctaagctctt tcttatggct taatcgaccg ctggcctgcc tgtttattct
     2641 ggttggcgcg gctgcacaat atttcataat gacttacggc atcgtcatcg accgctcgat
     2701 gattgccaat attattgata ccactccggc agaaagctat gcgctgatga caccgcaaat
     2761 gttattaacg ctgggattca gcggcgtgct tgctgcgctg attgcctgct ggattaaaat
     2821 caaacctgcc acctcgcgtc tgcgcagtgt tcttttccgt ggagccaata ttctggtttc
     2881 tgtactgctg attttgctgg tcgccgcact attttataaa gactacgcct cgttgttccg
     2941 caacaacaaa gagctggtga agtccttaag cccctctaac agcattgttg ccagctggtc
     3001 atggtactcc catcagcgac tggcaaatct gccgctggtg cgaattggtg aagacgcgca
     3061 ccgcaaccca ttaatgcaga acgaaaaacg taaaaatttg accatcctga ttgtcggcga
     3121 aacctcgcgg gcggagaact tctccctcaa cggctacccg cgtgaaacta acccgcggtt
     3181 ggcgaaagat aacgtggtct atttccctaa taccgcatct tgcggcacgg caacggcagt
     3241 ttcagtaccg tgcatgttct cggatatgcc gcgagagcac tacaaagaag agctggcaca
     3301 gcaccaggaa ggcgtgctgg atatcattca gcgagcgggc atcaacgtgc tgtggaatga
     3361 caacgatggc ggctgtaaag gtgcctgcga ccgcgtgcct caccagaacg tcaccgcgct
     3421 gaatctgcct gatcagtgca tcaacggcga atgctatgac gaagtgctgt tccacgggct
     3481 ggaagagtac atcaataacc tgcagggtga tggcgtgatt gtcttacaca ccatcggcag
     3541 ccacggtccg acctattaca accgctatcc gccgcagttc aggaaattta ccccaacctg
     3601 cgacaccaat gagatccaga cctgtaccaa agagcaactg gtgaacactt acgacaacac
     3661 gctggtttac gtcgactata ttgttgataa agcgattaat ctgctgaaag aacatcagga
     3721 taaatttacc accagcctgg tttatctttc tgaccacggt gaatcgttag gtgaaaatgg
     3781 catctatctg cacggtctgc cttatgccat cgccccggat agccaaaaac aggtgccgat
     3841 gctgctgtgg ctgtcggagg attatcaaaa acggtatcag gttgaccaga actgcctgca
     3901 aaaacaggcg caaacgcaac actattcaca agacaattta ttctccacgc tattgggatt
     3961 aactggcgtt gagacgaagt attaccaggc tgcggatgat attctgcaaa cttgcaggag
     4021 agtgagtgaa tgaaaattct gattgttgaa gacgatacgc tgttattgca gggactgatt
     4081 ctggcggcgc aaaccgaagg ctacgcgtgc gatggcgtga caaccgcgcg gatggcggaa
     4141 caaagccttg aggcaggtca ttacagcctg gtggtactgg atttagggtt acccgacgaa
     4201 gatggactgc attttctcgc ccgtatccgg cagaaaaaat ataccctgcc ggtactgatc
     4261 ctcaccgctc gcgatacgct gaccgacaaa atcgccgggc tggatgtcgg tgccgacgac
     4321 tatctggtga agccttttgc gctggaagag ttacatgccc gtatccgcgc cctgctacga
     4381 cgccataata atcagggcga aagtgagctg attgttggca atctgacgct gaacatgggt
     4441 cgccgtcagg tatggatggg cggtgaagag ttgattctga cgcccaaaga atatgctctg
     4501 ctgtcacggt taatgctcaa agcaggcagt ccggtgcatc gggaaattct ctacaacgac
     4561 atctataact gggacaatga accctcgacc aacaccctgg aagtgcatat ccacaatctg
     4621 cgcgacaaag tgggcaaagc ccgtatccgc accgtgcgcg gctttggcta tatgcttgtc
     4681 gcgaatgagg aaaactaatt gaatctgatg cgttttctgc gccgaccaat atcgctgcgc
     4741 caacggctga tattgaccat cggggccatt ttgttggtgt ttgagctgat cagcgtcttc
     4801 tggctatggc atgaaagtac cgagcagatt cagctgtttg agcaggcgct acgcgacaat
     4861 cgcaacaacg atcgccacat catgcgtgag atccgcgagg cggtcgccag cctgattgtc
     4921 cccggcgtct ttatggtcag cctgacgcta tttatctgct atcaggcggt acgccgcatc
     4981 acccgcccgc tggcggagct gcaaaaagag ctggaagcgc gcaccgccga caacttaacg
     5041 cccattgcca ttcacagcgc caccctcgaa atcgaagcgg tggtttcggc gttgaacgat
     5101 ctggtcagtc gcctgaccag cacgctggat aacgaaaggt tatttaccgc tgacgtcgcg
     5161 cacgaactgc gaacgccact ggcgggggtg cgtttgcatc tggaactgct ggcgaaaacg
     5221 catcacattg atgtagcacc gttagtggca cggcttgatc agatgatgga gagcgtctcc
     5281 cagctgctgc aactggcgcg tgccggacag tcattttctt ccggtaatta tcaacatgta
     5341 aaactgctgg aagatgtcat tctcccctcg tatgacgaac tcagtaccat gctcgaccag
     5401 cgacagcaaa ccctgctatt gccagagagc gccgccgaca tcaccgttca gggtgatgca
     5461 accctgctgc ggatgttatt gcgaaacctg gtagaaaacg cccatcgtta cagcccgcaa
     5521 ggcagcaaca ttatgattaa gctgcaagaa gatggcggag cggtcatggc cgttgaagat
     5581 gaaggaccgg gtattgatga gagtaaatgc ggggagttga gtaaagcgtt tgtacgtatg
     5641 gacagccgtt atggcgggat tggtctgggg ttaagtattg tcagccgcat tacacagttg
     5701 catcacgggc agtttttcct gcaaaaccgg caagagactt ccggcacgcg ggcctgggta
     5761 cggctgaaga aagatcagaa cgtggcaaac cagatataaa gaaagctgct gaccaccagc
     5821 acgctgaaca cggtagttaa actttcataa acacggtttt tcattgcact ctcctccgtt
     5881 aacctggagg agagtatgcg cgtcagagat taagtgaacc ttaagagttc aattggcctg
     5941 ataagacagc gttacatcag gccatccgtt tcagcttatt catcaattcg cggatgttgc
     6001 tgcaccaggc gggtacgttt cgcctgcaaa tcggcaatct cgtggtcaat atcatcgatt
     6061 ttctgctcga tattatcgta atgctcgacg agaatttcct tcgcttcctg tatatctgac
     6121 gccgccggtg ttgcaccttt caacggacga tttgccgtct ctttcatggt tacgccggtg
     6181 attaaaccaa ccaccgccac taccatcagg taataggctg gcatcatcag attctgcgag
     6241 ctttcgacca gccaggccgc cagtgttggc gtcagaccgg caaccagcac cgaaatatta
     6301 aatgccgccg ccagcgcgct gtaacggata tgcgtcggga acatcgctgg caaggtagaa
     6361 gccataacgc ccgtaaagca gttaaggatc accgccagca tcagtaaccc ggcaaaaatc
     6421 aggccgatga cgttactgtt aatcagaata aacgccggga tcgccaacac aaacagtgca
     6481 acactaccaa gtagcacaaa cggacgacgg ccaaaacggt cactcagcaa gcccatcacc
     6541 ggctggacaa acagcatacc gatcataatg gcgataataa tcagcacccc gtggtcttcg
     6601 gagtaatgca ggttatgcga caaataactc ggcatatagg tcagcaacat gtagtaagtc
     6661 acgttggtgg caattaccag accaatacat gtcaacaggc tgcgccagta tttagtggca
     6721 atctctttaa acgagacttt cgggccatcc tgcaaacctt cgcggtcgcc ctgttccagt
     6781 ttatcaacat gctgctggaa cgccggagtt tcttccagcg catggcgcag gtaaagcccg
     6841 ataatcccta acggcagagc aataaagaac ggaatacgcc agccccagtc gaggaagttc
     6901 gcttcgccga caatggtcga aattaacacc accacgcccg cacccagcac aaacccggca
     6961 atagaaccga agtccagcca gctgcccata aagccacgtt tacggtcagg ggagtattcc
     7021 gcaacaaata tcgacgcccc ggtatattca ccgccgaccg agaaaccttg tgccatctta
     7081 cagatcaaca gcagaatcgg tgcccaaata ccaatcgtgt cgtaggacgg tattaagcca
     7141 atacagaacg tactgatcga cataatcaca atagtgatag cgaggatctt ctggcgacca
     7201 tatttatcgc ccaacatacc aaagaagagt ccgccaagcg gtcgaatcag aaagggaacg
     7261 gagaaagtgg caagtgcagc aaccatctgc acgctggggt cagcccccgg gaaaaaaact
     7321 ttacctaatg cgtaagcaac aaaaccataa acaccaaaat caaaccattc cattgcatta
     7381 cccagtgatg ctgcggtaat ggctttacgc agtttaccgt catcaataat ggtgacatca
     7441 cgaagggtaa tcggttttac ttttttcctt ttcagcatag ctttcctcgc agagttggcc
     7501 catttccgca aacgccagaa aaatcagcgc gacggccgca aaaggggagc gggaattgca
     7561 ggatgcaatc tctgtaacaa gcgtagcggg tttgcttaca ccctccggtt aaggaatgta
     7621 caatcaaacc agttgacgcc tgaattgggc agttaatgac ctttttaccc taccagcgtt
     7681 taaatttgtg atcaacttca cacatggatt tcgtttagga ctcattgatg taactgatta
     7741 tttacaccaa atttacagag aagatttcct cgagcattgc ccccgccaga cgctgcgctg
     7801 aacagagtcg tggcatcccc agcgcgacag tttgccagcg ttgggtcact gaccagcaaa
     7861 cgggttgttt atctgccgtg gtccactttc ccagccaact taaaaaatca gcatgatccc
     7921 cgcccgtcgg aacggtcgaa gaggcaaacc actgatggta atagtgacgc gtggtcgggg
     7981 ccgcattgat actctctgcc agatgttgtg tcgctttcac gcgcaaatca cccagcaaca
     8041 acgccaccgc ttgcgggctt cccagacgac aggcatcgcc aaatgccccc caacgcaact
     8101 cttcgagaac gataaaacag cgcccaggca acgaccaggc gttatacgcc ccagcacgcc
     8161 agcgggtaaa aatttgctcg ctctgttcac ccgcctgcac cagtaggcct cgttgcgtta
     8221 acgccttttc cttccagcga caacgggctg aaaaccactc atacaaggcg gcaatttgct
     8281 gggggagctc cgggaaagca gagtgctggc ggtgatccat tttttgcagc agcgccagat
     8341 gctgcgcgat ctgcgtcagt gccagatgac cgggatccag catcgacgcc agtttttcca
     8401 ccaatgtcag acgcagacta agcggcgcat ttaacatccc gccgttcgtc catgtctgaa
     8461 gctgcacgcg cgcaagaagt tcctgcatca gtcgttcacg aaactgctgc tgatgggctt
     8521 gtggaaggcg ggcatcatcg ccctgcgcca gatccactaa aaagcgggga taaaccgact
     8581 ccagcacgcg accggggccg tccagtaacg tcttggtcaa tatcgttctg ccgtgaaaag
     8641 tgtttgaata tcatcgcgta acagctgagc gtcggtgtaa atccagccgt gagtcatcac
     8701 agtctgctgc aattgctgtt gcatcagcct gaccaccgat tcattttgtt gacgcagagc
     8761 cagactttcg cgtaaacgcg tctgtaattc cgtcaaacat gaagcgaact cagcgaaaaa
     8821 agtattcatg cctgccgtaa cagattcatc gacctgctct gccagaactt tagccatttg
     8881 ttggcaataa agatcgactt ctgcgcttaa tgctcgttgc aacacactgt aatcaaccgt
     8941 ttctgtcggg gatttctcat ttccccgtcc ccagtcaggc tgattcaacc agcgcgaaaa
     9001 agtctcacgc acaacgccta atcgcgtgct ctgctcgtcc gttgcttcct ggcgcgaaat
     9061 gacttcactg aacagctggc gagtgttgaa gtggggaact acgccgtgaa aaacaggaaa
     9121 atgaaaccca ggacgaaacc ctgactcgct caattccatt ttgacttgtt gctcaatggg
     9181 gcgaataaca tcggttaaca cccggcaaag ggtggattca agctcggcaa aacgcagcgt
     9241 aaagtcgcga ctgatggtgt tctgcgccgt ctgtaacagt gtctcacagc gggtacgaat
     9301 ctcgcttaac ggctccgaat catcctgaaa caaggcggct aactgcacct tcagcgcatc
     9361 ttgctgttga cgcagaaagt ggttggcgga ggtcagggcc agctcgattt catgtttaat
     9421 ctcgccgctc acctgcgcct gattgagttg caatagctgc aaactttctt cgatctggtg
     9481 gatattttgc cgcaattgtt cacaagcgac gtttaacccg tgcgcacgaa aatccaggta
     9541 ttcccgcgcc tgctgcgcgt aattcaacag tttatgcgcg gcagatcgca gagcatacaa
     9601 cgaggcgtta gcgtaagcgg catgaagcaa catctgaatt ggctgggcga acagcgaatc
     9661 ttcccacaac tgatcggcag catgacgaat atgttcgagg tccgccagat cggcatgacg
     9721 ccagcgcctg ccgagcgcgg catgggcaaa atcttccacc cagcgttgtt gctctggcgg
     9781 tggtaactta ccgttgttgg ctaactcatg gcgcgcccga tttgccaggt agccccacat
     9841 cgacgacacc ggaaatatct gctgtggcgt aatacagcct ttcatcagcg tcccggaaat
     9901 cagtgcccgc acctggtcgg cgtcgtcact gttacgatcc tgttgatcga acttattgac
     9961 cagcacatac agcggcaccg attgccccac cgccaaaatc gcctcacgga cctcttcatc
    10021 ggagatcgat ttcagttgcg tataatccag caccgccagt accgccgagg cgcgtgccag
    10081 ctgctggtta agcatttttt gcagatgcgg ttgcccggct tcatttggcc cgggggtatc
    10141 cagtaacgtc aattgaccgg gataactttc cagccccgcc agatggacaa actccacttc
    10201 aatcacggga atatgctcaa tggcggcgta agcagaaaaa ggaaaatcga cgtccagcgc
    10261 cttcgccagt cgcaccaaat cattcaaact tttcagacaa tgaaaaatag gctgggcacc
    10321 cagataatat ttttcgaaag cgacgccatt ttcgatccgc tgcataagcg cacgcatatc
    10381 tttatctatt tccagcacat cggtcagatg cttaatatcg caatcacgca ggcgctgttg
    10441 taatttttga attaaacaat cgattggcgc gacatgtgaa aaatgcagta ccggttcctt
    10501 ttggccgggc gtatggcgaa taagcgtcgg cagcgcagtc attgggcgat tgcgattagg
    10561 cagtacctcc gtaccaacaa tggcattaat ggtggttgat ttccctgctt tcatggtacc
    10621 gacaatcgcc agcaccattt ccagtcggga aattttacgc aactcattat tcagcatcgc
    10681 gtgacgctcg gcgatattag gctgactcca gggtaaagcc agttgtggcg catcgtctcc
    10741 gggtacagag agaggcattt tttccagtaa ctgcaactgt tggcgagaaa gctgtaacag
    10801 ccgttcagcc tcctgactta actcatacag ggtctgtgtg tacatagaaa attcttcctt
    10861 aaagcaaatt ttattacttt tatttagcca gattgttttt gagttctgtt ttcggctttt
    10921 ataattactg caagaaataa ttttatattt agagt
//
LOCUS       DBFIFI010000145        10907 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.145, whole genome shotgun sequence.
ACCESSION   DBFIFI010000145 DBFIFI010000000
VERSION     DBFIFI010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10907
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.145"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            246..749
                     /gene="dps"
                     /locus_tag="N7B39_003497"
                     /gene_synonym="pexB"
     CDS             246..749
                     /gene="dps"
                     /locus_tag="N7B39_003497"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HGU2423414.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            1153..1899
                     /gene="glnH"
                     /locus_tag="N7B39_003498"
     CDS             1153..1899
                     /gene="glnH"
                     /locus_tag="N7B39_003498"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HGU2423415.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            2037..2696
                     /gene="glnP"
                     /locus_tag="N7B39_003499"
     CDS             2037..2696
                     /gene="glnP"
                     /locus_tag="N7B39_003499"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HGU2423416.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            2693..3415
                     /gene="glnQ"
                     /locus_tag="N7B39_003500"
     CDS             2693..3415
                     /gene="glnQ"
                     /locus_tag="N7B39_003500"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HGU2423417.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            3532..5757
                     /gene="ybiO"
                     /locus_tag="N7B39_003501"
     CDS             3532..5757
                     /gene="ybiO"
                     /locus_tag="N7B39_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HGU2423418.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEF
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQILSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLGSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGATPAEPLPPGEPT
                     L"
     gene            complement(5754..6680)
                     /gene="rlmF"
                     /locus_tag="N7B39_003502"
     CDS             complement(5754..6680)
                     /gene="rlmF"
                     /locus_tag="N7B39_003502"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HGU2423419.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELKQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSECKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVTFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            6956..7216
                     /gene="mcbA"
                     /locus_tag="N7B39_003503"
     CDS             6956..7216
                     /gene="mcbA"
                     /locus_tag="N7B39_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HGU2423420.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            7481..9763
                     /locus_tag="N7B39_003504"
     CDS             7481..9763
                     /locus_tag="N7B39_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HGU2423421.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSIAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTATLNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTASIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPAGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            9805..10491
                     /gene="ybiX"
                     /locus_tag="N7B39_003505"
     CDS             9805..10491
                     /gene="ybiX"
                     /locus_tag="N7B39_003505"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HGU2423422.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFIWIQSMIRDDKKRAMLFELDKNIQNIQSLKSRYGENEEILSLLNLY
                     HNLLREWSEI"
     gene            10565..10831
                     /gene="ybiI"
                     /locus_tag="N7B39_003506"
     CDS             10565..10831
                     /gene="ybiI"
                     /locus_tag="N7B39_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HGU2423423.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKESQLC"
BASE COUNT         2678 a         2829 c         2748 g         2652 t
ORIGIN      
        1 cctctacacc gtctttatat atcgaattat gcaaaagcat atttattccg aaaattcctg
       61 gcgagcaaat aaataagaat tgttcttatc aatatatcta actcattgaa tctttattag
      121 ttttgttttt cacgcttgtt accactatta gtgtgatagg aacagccaga atagcggaac
      181 acatagccgg tgctatactt agtctcgtta attactggga cataacatca agaggatatg
      241 aaattatgag taccgctaaa ttagttaaat caaaagcgac caatctgctt tatacccgca
      301 acgatgtctc tgacagcgag aaaaaagcaa cagtagagtt gctgaatcgc caggttatcc
      361 agtttattga tctttctttg attaccaaac aagcgcactg gaacatgcgc ggcgctaact
      421 tcattgccgt acatgaaatg ctggatggct tccgcactgc actgatcgat catctggata
      481 ccatggcaga acgtgcagtg cagctgggcg gtgtagctct ggggaccact caagttatca
      541 acagcaaaac cccgctgaaa agttacccgc tggacatcca caacgttcag gatcacctga
      601 aagaactggc tgaccgttac gcaatcgtcg ctaatgacgt acgcaaagcg attggcgaag
      661 cgaaagatga cgacaccgca gatatcctga ccgccgcgtc tcgcgacctg gataaattcc
      721 tgtggtttat cgagtctaac atcgaataaa tccatcgctg atggtgcaga attttagtac
      781 ccgataaaag cggcttcctg acaggaggcc gttttgtttt gcagcccgcc tcaacgcact
      841 tatttagtgc atccatctgc tatccccagc tgattaagta aattttttgt atccacatca
      901 tcacacaatc gttacataaa gattgttttt tcatcaggtt ttacgctaaa taatcactgt
      961 gttgagtgca caattttagc gcaccagttt ggtgccccag aatggtgcat cttcagggtg
     1021 ttgccctata aatcgtgcat cacgtttttg ccgcatctcg aaaaatcaat gagttgcaaa
     1081 actggcacga ttttttcata tatgtgaatg tcacgcaggg gatcgtcccg tggatagaaa
     1141 aaaggaaatg ctatgaagtc tgtattaaaa gtttcactgg ctgcactgac cctggctttt
     1201 gcggtttctt ctcatgccgc ggataaaaaa ttagttgtcg cgacggatac cgccttcgtt
     1261 ccgtttgaat ttaaacaggg cgataaatat gtgggctttg acgttgatct gtgggctgcc
     1321 atcgctaaag agctgaagct ggattacgaa ctgaagccga tggatttcag tgggatcatt
     1381 ccggcactgc aaaccaaaaa cgtcgatctg gcgctggcgg gcattaccat caccgacgag
     1441 cgtaaaaaag cgatcgattt ctctgacggc tactacaaaa gcggcctgtt agtgatggtg
     1501 aaagctaaca ataacgatgt gaaaagcgtg aaagatctcg acgggaaagt ggttgcggtg
     1561 aagagcggca ctggctccgt tgattacgcg aaagcaaaca tcaaaactaa agatctgcgt
     1621 cagttcccga acatcgataa cgcctatatg gaactgggca ccaaccgcgc agacgccgtt
     1681 ctgcacgata cgccaaacat tctgtacttc atcaaaaccg ccggtaacgg tcagttcaaa
     1741 gcggtaggcg actctctgga agcgcagcaa tacggtattg cgttcccgaa aggtagcgac
     1801 gagctgcgtg acaaagtcaa cggcgcgttg aaaaccctgc gcgagaacgg aacttacaac
     1861 gaaatctaca aaaaatggtt cggtactgaa ccgaaataat aacgctacac ctgtaaagcg
     1921 cactggcagt tccctctccc ctctggggag aggattaggg tgagggcgca aacccgctcc
     1981 ggggccatta attaccctga atttgattat ttacaccacg gtaacaggaa caacatatgc
     2041 agtttgactg gagtgctatc tggcctgcca ttccgcttct gattgaaggt gccaaaatga
     2101 ccctgtggat atcggtcctc ggtctggctg gcggcctggt gatcggattg ctggcaggtt
     2161 ttgcacgcac cttcggtggt tggatagcca accacgtcgc gctggttttt attgaagtga
     2221 tccgcggcac acctatcgtc gtccaggtga tgtttatcta ttttgccctg ccgatggcgt
     2281 ttaatgactt acgcatcgac ccatttactg cggcggtggt caccatcatg atcaactccg
     2341 gcgcgtacat tgcggaaatc acgcgtggtg cggtgctgtc tatccacaaa ggttttcgtg
     2401 aagcaggact ggcgctcggt ctttcacgtt gggaaaccat tcgctacgtc attttaccgc
     2461 tggcactgcg tcgtatgctg ccgccgctgg gtaaccagtg gatcatcagc attaaagaca
     2521 cctcgctgtt tattgtgatc ggcgtggcgg aactgacccg tcaggggcaa gaaattattg
     2581 ccggtaactt ccgcgccctt gagatctgga gcgccgtggc ggtgttctat ctgattatta
     2641 ccctggtgct gagctttatt ctgcgtcgtc tggaaagaag gatgaaaatc ctgtgattga
     2701 atttaaaaac gtctccaagc actttggccc aacccaggtg ctgcacaata tcgatttgaa
     2761 cattgcccag ggcgaagtcg tggtgattat cgggccgtcc ggttccggta aatcgaccct
     2821 gctgcgctgc atcaacaaac tggaagaaat cacctccggc gatctgattg tcgatggcct
     2881 gaaggttaac gatccgaaag ttgacgagcg cctgattcgc caggaagcag gtatggtgtt
     2941 ccagcagttt tacctcttcc cgcatctgac ggcgctggaa aacgtcatgt ttggcccgct
     3001 acgcgtgcgt ggcgcgaaca aagaagaggc ggaaaaactg gcacgtgagc tgctggcgaa
     3061 agtcggtctg gcagaacgtg cacatcacta cccttccgaa ctttctggtg gtcaacagca
     3121 gcgtgtggcg attgcccgcg cgctggcggt gaagccgaaa atgatgctgt ttgatgaacc
     3181 gacttccgct cttgacccgg aactgcgcca tgaagtgctg aaggttatgc aagatctggc
     3241 tgaagaaggg atgacgatgg tgatcgtgac ccacgaaatc ggttttgccg agaaagtagc
     3301 ttcgcgcctg atctttatcg acaaaggccg gattgcggaa gatggcgatc cacaagtgtt
     3361 gattaaaaat ccgccgagcc agcgcttgca ggaattttta cagcacgtct cttaacttct
     3421 ctgcctccct ttcacccgaa agggaggtac accagattcc tctcatttaa aatcacccct
     3481 cccccagcat ctatacttat ctttttgctc tgttttctca ctggaggagt catgcggtgg
     3541 atcctgttca tcctcttctg cctgctgggc gcacctgccc acgcggtatc catacccggc
     3601 gttacaacca caacgacaac ggactcaacg actgaaccgg ccccggaacc ggatatcgaa
     3661 caaaaaaaag cggcttatgg cgcactggcg gatgtgctgg ataatgacac ctcgcgtaaa
     3721 gagttgatcg accagttgcg caccgtcgcc gcgacgccac ctgctgaacc ggtcccgaag
     3781 atcgtgccgc cgacgctggt tgaagagcaa accgtgctgc aaaaggtcac cgaattcagc
     3841 cgccattatg gtgaagccct ttccgcccgc ttcgggcagc tttatcgcaa tatcaccggc
     3901 tccccgcata agccgtttaa tccacaaacc ttcagcaatg cgctgaccca tttttcaatg
     3961 ttagcggttt tagtgtttgg tttttactgg ctgattcgcc tgtgcgcact gccgctgtat
     4021 cgcaaaatgg gtcagtgggc gcggcaaaaa aatcgtgagc gcagtaactg gctgcagctt
     4081 ccggcgatga ttatcggggc gtttattatc gacctgctgt tactggcact gacattattt
     4141 gtcggccaga tattaagcga caacctgaat gcaggcagtc gcaccatcgc tttccaacaa
     4201 agtttgtttc tcaacgcctt tgccctgatt gaatttttca aagccgtact acgcctgatt
     4261 ttttgcccaa acgtggcgga actgcgcccg ttcacgattc aggacgagag cgcccgttac
     4321 tggagtcgtc gcctgagctg gttaggcagt ttgataggtt atggcctgat tgtggccgtg
     4381 ccgattatct ctaatcaggt gaatgtacag ataggtgcgc tggcgaacgt catcattatg
     4441 ctgtgcatga ccgtctgggc gttgtacctg atctttcgta ataaaaaaga gattacccag
     4501 catttgctca actttgcgga gcattcgctg gcctttttca gcctgtttat ccgcgccttt
     4561 gcgctggtgt ggcactggct ggcaagcgcc tattttatcg tgctgttttt cttttcgttg
     4621 ttcgatccgg gcaacagcct gaaatttatg atgggtgcaa cagtgcgcag cctggcgatt
     4681 attggtatcg cagcatttgt ttccggtatg ttttcccgct ggctggcaaa aaccatcact
     4741 ctctcgccac atactcagcg taactatccg gagttgcaaa aacggctgaa tggctggctt
     4801 tcggcggcgc tgaaaacggc gcgtattctg accgtctgcg tggcggtaat gctgctgttg
     4861 agcgcatggg gattgttcga tttctggaac tggctgcaaa acggcgcggg gcagaaaacc
     4921 gtagatatcc tgatccgtat cgcactcatt cttttcttct cggcggttgg ctggacggtg
     4981 ctcgccagtt tgatcgaaaa ccggctggct tcggatattc atggccgccc gctacccagc
     5041 gcccgcacgc gtaccctgct tacgctgttt cgtaacgcgc tggcggtgat tatcagtacc
     5101 atcaccatca tgattgtgtt gtcggaaatc ggcgtcaata tcgcgccatt gctggcaggt
     5161 gccggggcat taggtctggc tatctcgttt ggttcgcaaa cgctggtgaa agatattatc
     5221 accggggtat ttattcagtt tgaaaacggc atgaacactg gagatttggt gactattggg
     5281 ccgttgaccg gcactgtgga acggatgtcg attcgctccg tgggcgtacg ccaggatacc
     5341 ggggcgtacc acatcattcc gtggtcttcg attaccacct ttgccaactt tgtccgcggc
     5401 attggttcgg tagtggcaaa ctacgatgtt gatcgccatg aagatgctga taaagccaat
     5461 caggcactga aagatgcggt agcggaatta atggaaaacg aagaaattcg tgggctgatt
     5521 attggtgaac cgaactttgc cgggattgtt ggcttaagca ataccgcgtt tacactgcgt
     5581 gtttcgttca ccacgctgcc actcaaacag tggacggtac gctttgccct cgacagccag
     5641 gtgaaaaaac atttcgacct ggcgggcgtt cgcgcgccgg tgcagactta tcaggtgctg
     5701 cctgctccgg gcgcgacccc ggctgaaccg ttaccgccgg gggagccaac gctttaacgc
     5761 tggcgattga caaaacggcg gcgctgctcg tcgttcataa aggtccaggc aataaagcga
     5821 ctctgctttt gcccctgggc catctctttt ttaaccacct tcaccgcgcc cacgtccgtc
     5881 agggcacgat acaacggcgg taagttttca ccacgagata ccagtgaagt aaaccacatc
     5941 acctgcttcg cgaagccttt gctctcttca atcatctttt tgataaaggt gacctcaccg
     6001 ccttcacacc acaactcctg ttgctggccg ccaaagttca gtgcatcgtc tttgttcagc
     6061 cccaggttac ggcgtttaca ctcactacct gcccgcgccg cagcggcgga atcgtggaat
     6121 ggcgggttac acaaggtcgc gtcgtattgc tcgtttttat ggatgatgcc gttaaaaatc
     6181 gccccactct ctttttgccg acgcagacga atggcgcgat taagccccgg attagcactg
     6241 atgatcgcct gcgcgctgct taacgcctgg ctgctggttt cactaccggt aaaacgccag
     6301 ccatattcat gtacgccaat cagcggataa atacagttcg caccaacgcc gatatcaaga
     6361 atgctggcat tagctggaat tgttccgctt gcttcagcca gtaaatcggc aaggtgatga
     6421 atataatccg cccggcccgg tactggcggg cagagaaaac cgtccgggat atcccagttc
     6481 gctacggcgt aaaaatgggc cagcaacgcc ttattgagcg ccttcaccgc cagcggattg
     6541 gcaaagtcta cgctttgctc cccggcgggt gtaagcgtga ggaattgctt gagttcagga
     6601 ttgacctgac aaagcgtggc gagatcgtag cggctgtgat gacggttgcg cggatgcaac
     6661 cccggtttct gggcggacat agcattctcc tttaagcagc ggcgtaagat acccgcataa
     6721 cgcgacaggg taaatatctg gctgcgttta tcattcccca aaaccttaac atggctatgg
     6781 tttatggcaa ttcatcataa aaatcagcat tatgcatgtc attgatttgt cttattttgt
     6841 aaaaatatat cgttcaaaaa ataagcacat ctctgtcaat tttttgcgtc atttgcatcg
     6901 cgcaggcgac ctacacttat caggcactac ccgttgtatt tatgaggatg tcattatgaa
     6961 aaagtgcctc acactactga ttgccaccgt cctgagcgga atctctctca cggcttatgc
     7021 cgcgcaaccg atgagtaacc tggacagcgg tcagctacgg cccgccggta ccgtttcggc
     7081 aactggcgca tcaaacctaa gcgatctgga ggataaactg gcagaaaaag cgcgcgagca
     7141 aggcgcgaaa ggttatgtca ttaattccgc aggcggaaat gaccagatgt tcggtactgc
     7201 aaccatctac aaataacccc accaacgtaa ctgcattcgc ctctggatgc agttttcgcc
     7261 tgttttgtaa tcaaaaaaca tcaaatatga acgcaatgta aataaatgta tttctttttc
     7321 gcgcaatggg tgatagaaaa tcgcgccaag tgataatgct tatcaaaatt attatcactt
     7381 gcacgaacac tatcacgaga ttaacagtgg catcgcatcc gcagagaggc tttctcgtgg
     7441 cagtgaaaat ttcaacatat aagaaaaagt cacctgcaaa atggaaaaca atcgcaattt
     7501 ccctgccaga caatttcatt cgctcacgtt ctttgccggt ctttgtattg gcatcacgcc
     7561 tgtggctcag gcactcgccg ccgaagggca aactaacgcg gatgacacgc tggttgtcga
     7621 agcatcaacg ccttcgcttt atgcgccaca acaatctgcc gatccgaaat tctcgcgtcc
     7681 ggtagcggat actacccgca cgatgacggt gatttctgaa caagtgatta aagatcaggg
     7741 cgcaaccaac cttaccgatg cgctcaaaaa cgtccccggc gtgggtgcgt tttttgcggg
     7801 tgagaacggt aactccacca ctggcgacgc catttatatg cgcggtgctg atacctctaa
     7861 cagtatttat attgatggca ttcgcgatat cggcagcgtc tcgcgcgaca ccttcaatac
     7921 tgagcaggtc gaagtgatta aagggccttc cggcaccgac tacgggcgca gcgcgccgac
     7981 gggctcgatc aatatgatca gcaagcagcc gcgcaatgat tccggcattg acgcctccgc
     8041 cagtattggc agcgcctggt tccgccgcgg cacgctggac gtcaatcagg tcattggtga
     8101 taccactgcg gtgcgcctga atgtgatggg cgaaaaaacg cacgatgccg gacgcgacaa
     8161 agtcaaaaat gagcgttacg gcgtcgcccc ttctatcgct tttggccttg gtacagcgaa
     8221 tcgtttgtat cttaattatc tgcatgtcac ccagcacaac acgccagacg gcggcattcc
     8281 aaccatcggt ttgccgggct attctgcccc atctgcagga acggcgaccc tgaatcattc
     8341 cggaaaagtt gatacgcata acttttacgg cacggattcc gattacgacg attcgaccac
     8401 cgacaccgcc accatgcgtt ttgagcacga tatcaacgat aacaccacca ttcgcaacac
     8461 tacccgttgg tcgcgcgtga agcaggatta cctgatgacg gcgattatgg gtggggcgtc
     8521 gaatattacc cagcccacca gcgatgtgaa tagctggacg tggtcacgca cggcgaatac
     8581 caaagatgtg agtaataaaa ttctcaccaa ccagaccaac ctgacctcga cgttctatac
     8641 cgcttctatc ggtcatgatg tcagtaccgg cgtggaattt acccgtgaaa cgcagactaa
     8701 ctacggcgtt aatccggtga cgttacctgc ggtaaatatt tatcatcctg acagcagcat
     8761 tcatcccggc ggcctgacgc gcaacggcgc aaacgccaat ggtcagacgg ataccttcgc
     8821 aatttacgca ttcgatacgc tgcaaatcac ccgtgatttt gagctgaacg gcgggatccg
     8881 tctggataat tatcatactg aatatgacag tgccaccgcc tgcggcggca gcggacgcgg
     8941 tgccatcacc tgcccagctg gtgtggcaaa aggttctccg gtcaccaccg tcgacaccgc
     9001 caagtcgggc aatctggtga actggaaagc cggggcgctg tatcacctga cggaaaacgg
     9061 caatgtctat attaactatg ccgtttccca gcagcctccg ggcggcaaca acttcgccct
     9121 tgcgcagtct ggcagcggta acagtgccaa ccgcaccgat tttaaaccgc aaaaagccaa
     9181 caccagcgag attggcacca aatggcaggt tctggataaa cgcctgttgc tcaccgccgc
     9241 gctgttccgt actgatatcg aaaatgaagt tgagcaaaat gatgacggga cttactcgca
     9301 atacggtaag aaacgcgtcg aaggctatga gatatccgtg gcagggaata tcactcccgc
     9361 gtggcaggtg attggcggct atacccagca aaaagcaacc atcaaaaacg gcaaagatgt
     9421 tgcccaggat ggttcctcat cgctgccgta taccccggag cacgccttca ccttatggag
     9481 ccaatatcag gcaaccgatg atatctctgt tggcgcgggc gcacgctata tcggcagtat
     9541 gcataaaggt tcagacggcg cggtgggaac gccagcgttt accgaaggtt actgggtcgc
     9601 cgatgccaaa ctggggtata gggttaatcg caatctcgac ttccagctaa acgtctacaa
     9661 cctgtttgat accgattacg tcgcctcaat caataagagc ggctaccgtt atcacccggg
     9721 cgagccaaga accttcttgc tcacagccaa tatgcatttc tgattcagat gtggggcgca
     9781 ggccccactt tttggagaac ttgtatgatg taccacattc ccggcgtgtt atcgccacag
     9841 gacgtcgccc gttttcgcga acaactggaa caagccgaat gggtggatgg acgcgtcacc
     9901 accggcgcac aaggtgcgca agttaagaac aatcaacagg tagacacccg cagcacgtta
     9961 tacgccgccc tgcaaaatga ggtgctgaac gcggttaacc aacatgcttt gttctttgcc
    10021 gcggccttgc cgcgtaccct ttccacgccg ctgtttaatc gctatcagaa caatgaaacc
    10081 tatggttttc atgtggatgg cgcagtacgc agccatccac aaaacggctg gatgcgcact
    10141 gacctttctg ccacgctgtt tttaagcgat ccacaaagct acgacggcgg cgaactggtc
    10201 gttaatgaca ccttcggaca acatcgggta aaactcccgg caggtgatct ggtgttgtat
    10261 ccctccagca gcctgcattg cgtgacaccc gtaacccgcg gcgtacgagt ggcatcattt
    10321 atatggatcc agtcgatgat ccgcgatgat aaaaagcgcg ccatgctgtt tgaactggac
    10381 aaaaatattc agaatattca gtcgctgaaa agccgctatg gtgaaaatga agagatcctg
    10441 tcgctgctca atctttatca taatctgctg cgggaatggt cggagatctg acgcttgaac
    10501 tgcccaggtt cgtccctata ttagctacac ttaactatac aagcattgat atggggagga
    10561 cgatatggca tccggttggg ctaacgatga cgccgtcaac gaacagatca acagtacaat
    10621 tgaagatgcg attgcccgcg ctcggggtga aattccgcgt ggcgaaagcc tgtatgaatg
    10681 tgaagagtgc ggtgccccca tcccgcaagc ccgtcgggaa gccattcctg gcgtgcgctt
    10741 atgtattcat tgtcagcagg agaaagattt acaaaaacca gcttatacag gatataatcg
    10801 cagaggttcg aaagaaagcc agttatgtta actacaactg gcgagatgta tagcgagtat
    10861 aatttgtatt ttgcgtggca ttccgtgcct ttaacgctgt cgtgact
//
LOCUS       DBFIFI010000146        10897 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.146, whole genome shotgun sequence.
ACCESSION   DBFIFI010000146 DBFIFI010000000
VERSION     DBFIFI010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10897
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.146"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..888
                     /locus_tag="N7B39_003507"
     CDS             <1..888
                     /locus_tag="N7B39_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HGU2423424.1"
                     /translation="LRENFNEQVDTNVNTIISILNRRDIILESLQPYLVINKDAVTPC
                     TFLGDQTGDRFSNICGDQFIIDLLKRIMSINDNVHVLAGNHETNCNGNYMQNFTRMKP
                     LDEDTYDGIKDYPVCFYDSKYKIMANHHGITFDDQRKRYIIGPITVSIDEMTNALDPV
                     ELAAIINKKHHTIINSKKFKTSRAISCRSFNRYFSVSTDYRPKLEALLACSQMLGINQ
                     VVAHNGNGGRECIGETGTVLGLNARDSKHTGRMFSMHNCQINPSAGPEITTPWKSYQH
                     EKNRNGLMPLIRRRTMLQL"
     gene            1078..1191
                     /gene="yhiY"
                     /locus_tag="N7B39_003508"
     CDS             1078..1191
                     /gene="yhiY"
                     /locus_tag="N7B39_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HGU2423425.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            1254..2321
                     /gene="yhiI"
                     /locus_tag="N7B39_003509"
     CDS             1254..2321
                     /gene="yhiI"
                     /locus_tag="N7B39_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HGU2423426.1"
                     /translation="MDKSKRHLAWWGVGLLAVAAIVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            2318..5053
                     /gene="rbbA"
                     /locus_tag="N7B39_003510"
     CDS             2318..5053
                     /gene="rbbA"
                     /locus_tag="N7B39_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HGU2423427.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVIVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEASPVIHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPLLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPSSQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            5053..6177
                     /gene="yhhJ"
                     /locus_tag="N7B39_003511"
     CDS             5053..6177
                     /gene="yhhJ"
                     /locus_tag="N7B39_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU2423428.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLIMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            6250..6525
                     /locus_tag="N7B39_003512"
     CDS             6250..6525
                     /locus_tag="N7B39_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HGU2423429.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            6522..6881
                     /locus_tag="N7B39_003513"
     CDS             6522..6881
                     /locus_tag="N7B39_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HGU2423430.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(6931..7791)
                     /locus_tag="N7B39_003514"
     CDS             complement(6931..7791)
                     /locus_tag="N7B39_003514"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase"
                     /protein_id="HGU2423431.1"
                     /translation="MPLVNGRILLDRIQEKRVLAGAFNTTNLETTISILNAIERSGLP
                     NFIQIAPTNAQLSGYDYIYEIVKRHADKMDVPVSLHLDHGKTQEDVKQAVRAGFTSVM
                     IDGAAFSFEENIAFTQEAVDFCKSYGVPVEAELGAILGKEDDHVSEADCKTEPEKVKT
                     FVERTGCDMLAVSIGNVHGLDDIPRIDIPLLKRIAEVCPVPLVIHGGSGIAPEILRSF
                     VNYRVAKVNIASDLRKAFITAVGKAYVNNHNEANLARVMASAKNAVEEDVYSKILMMN
                     EGHRLVRKAS"
     gene            complement(7823..8092)
                     /locus_tag="N7B39_003515"
     CDS             complement(7823..8092)
                     /locus_tag="N7B39_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HGU2423432.1"
                     /translation="MLNKTVEVRNSTGLHARPAACLAKAAKKYSCKVTLHYEGNDINA
                     TSMMNIMRAGIKGGKTVEIRCDGDDENEAIQTLTDLFRDRFGEAE"
     gene            complement(8082..9590)
                     /locus_tag="N7B39_003516"
     CDS             complement(8082..9590)
                     /locus_tag="N7B39_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HGU2423433.1"
                     /translation="MPDNSAAIVIDIGTTNCKVTCFSCLDATTLGAHKFVTAKQISPQ
                     GDVDFDIDALWQEVRQAIAQLNAASPLPVRRISIASFGESGVFLDEHGEILTPMLAWY
                     DRRGEEYLATLSEADSAALYDICGLPLHSNYSAFKMRWLLEHYPLRNRRGLRWLHAPE
                     VLLWRLTGEQHTDITLASRTLCLDVRKGEWSAKAAALLHVPCTAFAPLVQPGEHAGWV
                     SESLCKTLGFSQPVSVTLAGHDHMVGARALQMMPGDILNSTGTTEGILQLDTQPTLDE
                     QAKRDKLANGCYSLANQFTLFASLPVGGFALEWLRNTFRLTDEEIAASLTRGHADYLA
                     GNWLLDDIPVFIPHLRGSGSPYKNRHTRGLFYGLGDTLSIDMLIASVSLGLTMEFANC
                     FACFNVPGTSALKVIGPATHNPLWLQLKADILQRPVEAIAFNEAVSVGALLTAAPDIP
                     PPPVAIAQRLLPNRARYHQLQRYQHKWKSWYQLKLQQEGVMPLHHREEHYVE"
     gene            complement(9583..10896)
                     /locus_tag="N7B39_003517"
                     /pseudo
     CDS             complement(9583..10896)
                     /locus_tag="N7B39_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997818.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
BASE COUNT         2657 a         2740 c         2909 g         2591 t
ORIGIN      
        1 ctaagggaga actttaatga gcaagttgat actaatgtta atacaataat cagtatcctg
       61 aacagaagag acattatttt agaaagtctt cagccttatt tagttattaa taaagacgca
      121 gtgacgccat gtacgttttt gggtgaccaa accggcgatc gatttagtaa tatctgtggc
      181 gatcaattta tcatcgattt gttaaaacgc ataatgagca tcaatgataa cgttcatgta
      241 ttagctggaa atcatgaaac gaattgcaat ggtaactata tgcagaactt cacgcgtatg
      301 aaaccacttg atgaggatac ctacgacggg ataaaagatt atcctgtatg tttctatgac
      361 tccaaataca aaataatggc aaatcatcat gggataactt ttgatgatca gcgaaaacgt
      421 tacattatag gaccgataac ggtctctatt gatgaaatga ccaatgctct cgatccggtc
      481 gaattagcgg caattattaa taaaaagcat catactataa ttaatagtaa aaagtttaaa
      541 actagccggg ctatctcttg tcgatccttt aatcgttatt tttctgtttc gacagattac
      601 agacctaaac ttgaagcact attagcatgc tcacaaatgt taggtataaa tcaagttgtt
      661 gcacataacg gtaatggtgg aagagaatgc ataggtgaaa cggggacagt tttaggtctt
      721 aacgcccgtg acagtaaaca tacgggaaga atgttcagca tgcataattg ccaaattaac
      781 cctagtgcgg ggcctgagat aaccactccc tggaagtctt accaacatga aaaaaacaga
      841 aatggactta tgcctttgat tagaaggcgc acaatgttac agctctgaac catagacaac
      901 atgaatatta atgttactat ctggttgaga ctgttgataa aacgtaataa ggaatgctct
      961 gttcctgcaa ctgaacaggc gtgaaacgct atccaacagg atggataccg ttttgtcagc
     1021 cagttttata gatcacactt atgagtatgc gtgtttcatt tacggccagg aatgctgatg
     1081 atgacaacat tactacccgt tttcactaag ccttccccgt tggcgctcaa tgctctgcgc
     1141 gctggtcgta tttgtcgttt ccttcttatc ccggatggaa gaatccgctg aacgtcgttc
     1201 ggcggcagtt gtaacacgcg tcgtttgctt acagggatta tggcgggtcg attatggata
     1261 agagtaagcg ccatctggcg tggtggggtg tcgggttact ggcggtggct gctatcgtag
     1321 cgtggtggct gttgcgcccg gcaggtgtgc cggaaggctt tgccgtcagc aatgggcgca
     1381 ttgaagcgac ggaagtggat atcgccagca aaattgccgg gcgtatcgac accattctgg
     1441 tgaaagaagg ccagtttgtt cgcgaaggcg aagtgctggc gaagatggat actcgcgtgt
     1501 tgcaggagca gcgactggaa gccatcgcgc aaatcaaaga ggcacaaagc gccgttgccg
     1561 ccgcgcaggc tttgctggag caacgacaaa gcgaaactcg cgccgcacag tcgctggtta
     1621 atcaacgcca ggccgaactg gactccgttg ccaaacgtca tacgcgttcc cgctcgctcg
     1681 cccaacgagg ggctatttct gcgcaacagc tggatgacga tcgcgccgcc gctgagagcg
     1741 cccgagctgc gctggaatca gcaaaagccc aggtttcggc ttctaaagcc gctatagaag
     1801 cggcacgcac caatatcatt caggcgcaaa cccgcgtcga agccgcacaa gccactgaac
     1861 ggcgcattgc cgcagatatc gatgacagcg aactgaaagc cccgcgtgac ggacgcgtgc
     1921 agtatcgggt tgccgagcca ggggaagtgc tggcggcagg cggtcgggtg ctgaatatgg
     1981 tcgatctcag cgacgtctat atgaccttct tcctgccaac cgaacaggcg ggcacgctga
     2041 aacttggcgg tgaagcccgg ctgatcctcg atgccgcgcc agatctgcgt attcctgcaa
     2101 ccatcagttt tgtcgccagt gtcgcccagt tcacgccaaa aaccgtcgaa accagcgatg
     2161 aacggctgaa actgatgttc cgcgtcaaag cgcgtatccc accggaatta ctccagcagc
     2221 atctggaata tgtcaaaacg ggcttgccgg gcgtagcgtg ggtgcgggtg aatgaagaac
     2281 ttccgtggcc tgacgacctc gtggtgaggt tgccgcaatg acgcatctgg aactggttcc
     2341 cgtcccgcct gtcgcgcaac tggcgggcgt gagccagcat tatggaaaaa ccgttgcgct
     2401 gaacaatatc acactcgata ttccggcccg ctgtatggtc gggctgattg gtccggatgg
     2461 cgtcgggaag tcgagtttat tgtcattgat ttccggtgct cgtgtcattg agcagggcaa
     2521 tgtgatcgtg ctgggcggcg atatgcgcga cccgaagcat cgccgcgacg tctgcccgcg
     2581 catcgcctgg atgccgcagg ggctgggcaa aaacctctac cacaccttgt cggtgtatga
     2641 aaacgtcgat tttttcgctc gcctgttcgg tcacgacaaa gcggagcggg aagtgcgaat
     2701 caatgagctg ctgaccagca ccgggttagc accgtttcgc gatcgtccgg cagggaaact
     2761 ctccggcggg atgaagcaaa aactggggct gtgctgtgcg ttaatccacg acccggaact
     2821 gttgattctt gatgagccta cgacgggggt tgacccgctc tcccgcgccc agttctggga
     2881 tctgatcgac agtattcgcc agcggcagag caatatgagc gttctggtcg ccacggccta
     2941 tatggaagag gcggaacgct tcgactggtt ggtggcgatg aatgccggag aagtgttggc
     3001 aactggcagc gccgaagagc tgcggcagca aacgcaaagc gccacgctgg aagaagcatt
     3061 tataaatctg ttaccgcaag cgcaacgcca ggcgcatcag gcggtagtga tcccaccata
     3121 tcaacctgaa aacgcagaga ttgccatcga agcgcgcgat ctgaccatgc gttttggttc
     3181 cttcgttgcc gttgatcacg tcaatttccg cattccacgc ggggagattt ttggttttct
     3241 tggttcgaac ggctgcggta aatccaccac catgaaaatg cttaccggat tgctgcccgc
     3301 cagcgaaggt gaggcgtggc tgttcgggca accggttgat ccaaaagata tcgatactcg
     3361 ccgtcgggtg ggctatatgt cgcaggcgtt ttcgctctat aacgagctca ccgtgcggca
     3421 aaaccttgag ttacatgccc gtttgtttca catcccggaa gcggaaattc ccgcaagagt
     3481 ggctgaaatg agcgagcgtt ttaagctcaa cgacgttgaa gatgttctgc cggagtcatt
     3541 gccgctcggc attcgccagc ggctttcgct ggcggtggcg gtgattcatc gcccggaaat
     3601 gttaatcctc gatgaaccta cttctggcgt cgatccggtg gcgagggata tgttctggca
     3661 gttgatggtc gatctctcgc gccaggacaa agtgactatc ttcatctcca cccactttat
     3721 gaacgaagcg gaacgctgcg accgtatctc tctgatgcac gccggaaaag tgctcgccag
     3781 cggtacaccg caggaactgg ttgagaaacg cggagccgcc agtctggaag aggcatttat
     3841 cgcctatttg caggaagcgg caggacagag caacgaagcc gaagcgtctc ccgtgataca
     3901 cgacaccaca cacgcgccgc gccagggatt tagcctgcgc cgtctgttta gctacagccg
     3961 ccgcgaagcg ctggaactgc gacgcgatcc ggtacgttcg acgctggcgc tgatgggaac
     4021 agtgatcctg atgctgataa tgggttacgg catcagtatg gatgtggaaa acctgcgctt
     4081 tgcggtgctt gaccgcgacc agaccgtcag tagccaggcg tggacgctca atctctccgg
     4141 ttcccgttac tttatcgaac agccgctgct taccagttat gacgagcttg atcgtcggat
     4201 gcgtgcgggc gatatcacgg tggcaattga gatcccgccc aatttcgggc gcgatatcgc
     4261 gcgtggtacg cctgtggaac tcggtgtctg gatcgacggt gcgatgccga gccgcgccga
     4321 aactgtaaaa ggttacgtgc aggccatgca ccagagctgg ctacaggatg tggcgagccg
     4381 acaatccaca ccctccagcc aaagcgggct gatgaatatt gagacgcgtt atcgctataa
     4441 cccggacgta aaaagcctgc cagcgattgt tccggcggtg atcccgcttc tgctgatgat
     4501 gatcccgtca atgctaagcg cccttagcgt ggtgcgggaa aaagagctag ggtcgattat
     4561 caacctttac gtgaccccca ccacccgtag cgaatttttg cttggcaaac agctgccgta
     4621 catcgcgctg gggatgctga actttttcct gctctgcggc ctgtcggtgt ttgtgtttgg
     4681 cgtaccgcat aaaggcagtt tcctgacgct caccctggcg gcgctgctgt atatcatcat
     4741 cgccaccgga atggggctgc tgatctccac ctttatgaaa agccagattg ccgccatttt
     4801 cggtacggcg attatcacgt tgatcccggc gacacagttt tccgggatga tcgatccggt
     4861 agcttcgctg gaagggccgg gacgttggat cggcgaggtt tacccgacca gtcattttct
     4921 gactatcgcc cgcgggacgt tctcgaaagc gctggatctg actgatttgt ggcaactttt
     4981 tatcccgttg ctgatagcca taccgctggt gatgggctta agcatcctgc tgctgaaaaa
     5041 acaggaggga tgatgcgcca tttacgcaat atttttaatc tgggtatcaa agagttgcgc
     5101 agtctactcg gtgataaagc gatgctgaca ttgattgtct tctcgtttac ggtgtcggtg
     5161 tattcgtcag cgaccgtcac gccaggatcg ttgaacctgg caccgatcgc cattgccgat
     5221 atggatcaat cgcagttatc gaaccggatc gttaacagct tctatcgccc gtggtttttg
     5281 ccaccggaga tgatcaccgc cgatgagatg gatgccggac tggacgccgg gcgttatacc
     5341 tttgcgataa atattccgcc taattttcag cgtgatgtcc tcgccggacg ccagccagat
     5401 attcaggtga acgtcgatgc cacgcgcatg agccaggcat ttaccggcaa tgggtatatc
     5461 cagaatatta tcaacggtga agtgaacagc tttgtcgcgc gctaccgtga taacagcgaa
     5521 ccgttggtat cgctggaaac ccggatgcgc tttaacccga accttgatcc cgcgtggttt
     5581 ggcggggtga tggcgatcat caacaacatt accatgctgg cgattgtatt gaccggatcg
     5641 gcgctgatcc gcgagcgtga acacggcacg gtggaacact tactggtgat gccgataacg
     5701 ccgtttgaga tcatgatggc gaagatctgg tcgatggggt tggtggtgct ggtggtatcg
     5761 ggattatcac tggtgctgat ggtaaaaggc gtgttgggcg taccgattga aggctcgatc
     5821 ccgctgttta tgctgggcgt ggcgcttagt ctgtttgcca ccacgtcaat cggcattttt
     5881 atggggacaa tagcgcgttc aatgccgcaa ctggggctgc tggtgattct ggtgctgctg
     5941 ccgctgcaaa tgctttccgg tggttccacg ccgcgcgaaa gtatgccgca gatggtgcag
     6001 gacattatgc tgatcatgcc gacgacacac tttgttagcc tcgcgcaggc cattctctac
     6061 cgtggtgccg gattcgaaat cgtctggccg cagttcctga cgctgatggc aattggcggc
     6121 gcgtttttca ccattgcgct gctgcgattc aggaagacga ttgggacaat ggcgtaagga
     6181 agggaaactt gacttcatac tgtgatgtca tactattgat gtcataaagt gaagtcataa
     6241 ggacatgcca tgagaaccaa ggtacaggct ttacggaaga aacaaaaaaa tacattggat
     6301 caaatattta aaactcctgt tcctcaaggg atcaagtggt ctgatataga gtcactggtt
     6361 aaagcattag gcggagaaat taaggaagga agaggttcgc gttgtaagtt catactaaat
     6421 atgagcgttg cgtgtttcca tcggcctcat ccgtcgccag ataccgataa aggcgctgta
     6481 gaaagcgtgc gtgactggtt actaagtata ggggtaaaac catgatcaaa ctgaagacgc
     6541 caaactctat ggagattgcc gggcaacctg ctgttatcac ttacgtgcca gaactgaatg
     6601 catttcgtgg caaattcctg ggcttgtcag gttattgtga ttttgtctct gacagcattc
     6661 aggggctgca aaaagaaggg gaactttccc tgcgagagta tcttgaggat tgtaaagctg
     6721 cgggtattga gccgtatgcg cgtacagaaa agattaagac ctttacgtta cgctatccag
     6781 aatcgctgag tgaacgcctt aataatgcgg ctgcgcagca acaagtctct gttaatacct
     6841 atattattga aacactgaat gagcgtttga atcatttata acttatagcg gcccaacaat
     6901 ttctgttgcg ccgctatatt tttgtggctc ttaagatgcc tttctaacca accgatgccc
     6961 ctcattcatc atcaggattt tgctatacac atcttcctcg acggcgtttt ttgctgatgc
     7021 catgactcgt gccagattcg cctcgttgtg attattcaca tacgctttgc cgacggcggt
     7081 gataaacgct ttgcgcagat cgctggcgat atttacttta gcgacgcgat aattaacaaa
     7141 gctgcgtaat atctccgggg cgataccgga gccgccgtga ataaccaacg gcaccggaca
     7201 gacttcggca atgcgcttca gcagggggat atcgatacgc ggtatatcgt ccaggccatg
     7261 aacgttgcca atagaaactg ccagcatgtc gcaaccggta cgctcgacaa aggtcttcac
     7321 cttttccggt tccgttttgc aatcggcttc gctgacgtga tcatcttctt tgccgagaat
     7381 cgcccccagc tccgcttcaa ccggtacgcc ataggattta cagaaatcga cagcttcctg
     7441 ggtgaaagcg atattctctt caaaagagaa tgcggcaccg tcaatcatga ccgaggtaaa
     7501 gcccgcgcgc accgcctgtt taacatcctc ctgggttttc ccgtgatcca gatgaagact
     7561 gaccggaaca tccattttat cggcgtgacg tttcacgatt tcataaatgt aatcgtatcc
     7621 cgagagttgt gcattggttg gcgcaatctg aatgaagttg ggtaatcccg aacgctcaat
     7681 agcgtttaaa attgaaattg tcgtctccag attggtggta ttaaatgcgc ccgctaatac
     7741 ccgtttttcc tgaatgcggt cgagcagtat ccgtccatta actaatggca tattgaaaac
     7801 ctcatctttt aatatcagtt aattattcag cttcaccgaa gcggtcgcga aataagtcgg
     7861 tgagagtctg tatggcctcg ttttcatcat caccgtcgca gcgtatttcc accgtttttc
     7921 cgccttttat tccggcgcgc ataatattca tcatgctggt ggcattaata tcattaccct
     7981 catagtgcag cgtcacttta cagctatatt ttttggcggc tttcgccaga caggccgccg
     8041 gacgtgcatg caggccagta ctgtttctga cctccacggt tttattcaac atagtgttcc
     8101 tctcgatgat gtaatggcat cacgccttct tgttgtaatt tcaactgata ccagcttttc
     8161 catttgtgct gataacgctg taattgatgg tatctcgccc gattcggtaa caaacgttgg
     8221 gctatagcga ctggcggcgg tggaatatcc ggtgcggcgg ttaataatgc tccgacagaa
     8281 accgcctcgt taaatgcaat tgcttcaacc ggacgctgta aaatatccgc ctttaattgc
     8341 agccaaagag gattatgggt tgccggaccg atcactttta acgcgctggt gccaggcacg
     8401 ttaaaacagg cgaagcagtt ggcaaattcc atggttaatc ccagtgatac gctggcaatt
     8461 aacatgtcga tacttaacgt atcgccaagc ccataaaata atccacgggt atggcgattt
     8521 ttatagggcg aacccgaacc gcgaagatgt ggaataaaga cgggaatgtc atcgagcaac
     8581 caattccccg ccagataatc agcatgtccg cgagtaagtg atgcggcgat ctcctcatcg
     8641 gttagccgga acgtgttgcg cagccactcc agcgcgaaac cgcccacggg cagcgacgca
     8701 aacagggtga actggttggc aagtgagtaa cagccgtttg ccagcttgtc acgtttggcc
     8761 tgttcatcca gcgtcggttg tgtatccagt tgcagaatgc cttccgtggt ccccgtcgag
     8821 ttaaggatat cgcctggcat catctgcaac gctcgcgcac ccaccatatg gtcatgtccg
     8881 gccagcgtca cgctgaccgg ttgcgagaac ccaagcgtct tgcaaagtga ctcgctgacc
     8941 catccggcgt gctcgcctgg ctgcaccaat ggcgcaaatg ccgtacaggg aacgtgtaac
     9001 aacgccgccg ctttcgctga ccattcgcct ttgcgcacgt ccagacacag cgtgcggctg
     9061 gctaaggtga tatccgtgtg ctgttcgcca gtcagccgcc agagcagcac ttccggcgca
     9121 tgtagccagc gcaggccgcg gcgattacgc agcgggtaat gttccagcaa ccagcgcatt
     9181 ttgaaggcag agtaattgct gtgtagtggt agaccgcaga tgtcataaag tgccgcactg
     9241 tctgcctcgc taagcgttgc cagatactct tcaccgcgac ggtcatacca tgccagcatt
     9301 ggcgtcagga tctcgccatg ctcgtcaagg aacacgcctg attcgccaaa actggcaatg
     9361 ctgatccgtc tgactggcag cggcgaagcg gcgttcagtt gcgctatcgc ctggcggacc
     9421 tcctgccaga gggcgtcgat atcgaaatcg acatcgccct gtggggagat ctgctttgcc
     9481 gtcacgaatt tatgcgcgcc caacgtcgtt gcgtccaggc aggaaaagca ggtgactttg
     9541 caattggtgg tgccaatatc gataacgata gctgcgctgt tatcaggcat aaaccgtgct
     9601 ccgcgtcatt cggaaaatgc catacagtac cagggcgata accggcacca gaccaatggc
     9661 gatgagattg ccgttgaaga tttccagcag ccagaagcgg aacgggttac cgccatcaag
     9721 aaaactggaa acctggccgc tggagccttc ggcaaatttg aagttaacgt ctttcgccat
     9781 tccggtaata aacggcgcga ttttggtagc aatccatagg tcggcaataa tcaccgggat
     9841 cgccgccaga acggtgcgga aaatattgcc acggctggct aaagcaatca tcgacgccat
     9901 taccgccagg ttggcaaggt cgccaagcgg caggacttca ttacccggaa ggacaaacgc
     9961 gataaagacc gagattggcg ttagcagcag ggcggtggag ataatcgccg gattacctac
    10021 ggcaacggcg atatccaggc cgatatagag atcgtcacgg tcagggtatt tggcattgag
    10081 atacttttta atagcttcag acagcggtaa taaaccttcc atcaggatgc gtaccatgcg
    10141 tggcaggatg aacatcacac cgccaatgct gatgccaagc agcaatactt ttttgaaatc
    10201 gtatccggca attacgccga gcagaatgcc cagaatagtg ccaaccatca tcggttcgcc
    10261 gaagatgcca aactttttct gtacggtttc cgggtcgata tgaatacgat tgaggccagg
    10321 gatatggtcg atgattttgt cgaccagcag accgaccggg aagaacacca ccgaagagag
    10381 cgtcggcaat gagatccctt ccagtccaaa gtatttttgt accagtggcg cagaccagtc
    10441 ggcgagttta agtactacca ccgcagcaat cgccgccgcc agcaaaccaa agaacaggct
    10501 gccggtgctg taataaacca acgtaccagc aagggcaaaa tgccagtagt tctacatatc
    10561 tacgtcaacg gtacgggttt tatttaacgc cagcatcgcc acgttaatca gaatggtcag
    10621 gggaataaca aacggggcaa ttggcgaacc ccatgtaata gcggaaagcg gcggccagcc
    10681 taaatctacc accggtaaat taattccggt gcgttcaacc atcgcctggg ccgctggccc
    10741 gacattgctg gtcagaatat cgaaaatggc ataaataccg acaaaaccaa tgccgactgt
    10801 taatgacgaa cgtaaggctt tagcgggttt tactttaaaa cagagagcaa gaataaataa
    10861 caccaacggc aacattaccg tcgggccaaa tcccaat
//
LOCUS       DBFIFI010000147        10825 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.147, whole genome shotgun sequence.
ACCESSION   DBFIFI010000147 DBFIFI010000000
VERSION     DBFIFI010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10825
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.147"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            108..758
                     /gene="csgD"
                     /locus_tag="N7B39_003518"
     CDS             108..758
                     /gene="csgD"
                     /locus_tag="N7B39_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HGU2423434.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            763..1152
                     /gene="csgE"
                     /locus_tag="N7B39_003519"
     CDS             763..1152
                     /gene="csgE"
                     /locus_tag="N7B39_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HGU2423435.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            1177..1593
                     /gene="csgF"
                     /locus_tag="N7B39_003520"
     CDS             1177..1593
                     /gene="csgF"
                     /locus_tag="N7B39_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HGU2423436.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1620..2453
                     /gene="csgG"
                     /locus_tag="N7B39_003521"
     CDS             1620..2453
                     /gene="csgG"
                     /locus_tag="N7B39_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HGU2423437.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVTQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2517..3008)
                     /gene="ycdZ"
                     /locus_tag="N7B39_003522"
     CDS             complement(2517..3008)
                     /gene="ycdZ"
                     /locus_tag="N7B39_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HGU2423438.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILSYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLIFGYAMKNSGLWLAARSAKTALREQEIK
                     NKA"
     gene            complement(3110..3664)
                     /gene="ycdY"
                     /locus_tag="N7B39_003523"
     CDS             complement(3110..3664)
                     /gene="ycdY"
                     /locus_tag="N7B39_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HGU2423439.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3688..4425)
                     /gene="ycdX"
                     /locus_tag="N7B39_003524"
     CDS             complement(3688..4425)
                     /gene="ycdX"
                     /locus_tag="N7B39_003524"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HGU2423440.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4480..5418)
                     /gene="ghrA"
                     /locus_tag="N7B39_003525"
     CDS             complement(4480..5418)
                     /gene="ghrA"
                     /locus_tag="N7B39_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HGU2423441.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALA
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLKPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGWEELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            5652..5739
                     /locus_tag="N7B39_003526"
     tRNA            5652..5739
                     /locus_tag="N7B39_003526"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5686..5688,aa:Ser,seq:gga)
     gene            complement(5889..6731)
                     /locus_tag="N7B39_003527"
     CDS             complement(5889..6731)
                     /locus_tag="N7B39_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HGU2423442.1"
                     /translation="MSDITISRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISVLTESIGGKTAPDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDTQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLN
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGQQADRLADLERMLHLFSGKPIPDNR
                     GNITLRLDDHIQSVQGKESYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIAKHYPD
                     MLAV"
     gene            complement(6816..7013)
                     /locus_tag="N7B39_003528"
     CDS             complement(6816..7013)
                     /locus_tag="N7B39_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HGU2423443.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(7025..7516)
                     /locus_tag="N7B39_003529"
     CDS             complement(7025..7516)
                     /locus_tag="N7B39_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HGU2423444.1"
                     /translation="MMKLALTLEADSVNVQALNMGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGNGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(7513..7887)
                     /locus_tag="N7B39_003530"
     CDS             complement(7513..7887)
                     /locus_tag="N7B39_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HGU2423445.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(7934..8308)
                     /locus_tag="N7B39_003531"
     CDS             complement(7934..8308)
                     /locus_tag="N7B39_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HGU2423446.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLNAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            complement(8471..8692)
                     /locus_tag="N7B39_003532"
     CDS             complement(8471..8692)
                     /locus_tag="N7B39_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HGU2423447.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(8755..9231)
                     /locus_tag="N7B39_003533"
     CDS             complement(8755..9231)
                     /locus_tag="N7B39_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HGU2423448.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(9247..9732)
                     /locus_tag="N7B39_003534"
     CDS             complement(9247..9732)
                     /locus_tag="N7B39_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HGU2423449.1"
                     /translation="MTTVSHNSTTPSVSVTTASGNNPPQLVATPVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(9823..10641)
                     /locus_tag="N7B39_003535"
     CDS             complement(9823..10641)
                     /locus_tag="N7B39_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HGU2423450.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMRHVPSIFGENRHTSRSEHY
                     AYIPTITVLENLQQEGFQPFFACQTRVRDQSRREYTKHMLRLRRAGQITGQHVPEIIL
                     LNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            10735..10825
                     /locus_tag="N7B39_003536"
                     /pseudo
     CDS             10735..10825
                     /locus_tag="N7B39_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439280.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2728 a         2708 c         2710 g         2679 t
ORIGIN      
        1 tgatttatta ctacacacag cagtgcaaca tctgtcagta cttctggtgc ttctatttta
       61 gaggcagctg tcaggtgtgc gatcaataaa aaaagcgggg tttcatcatg tttaatgaag
      121 tccatagtat tcatggtcat acattattgt tgatcactaa accttctttg caggcgacag
      181 ctctcttgca gcaccttaaa caatcgctgg caattacagg aaaattacat aatattcaac
      241 gttctctgga cgatatctct tcaggctcta ttattcttct ggatatgatg gaagcggata
      301 aaaagcttat ccattattgg caggatactt tgagcaggaa aaacaacaat atcaaaatat
      361 tgttgctaaa tacgcctgaa gattacccgt accgcgacat tgaaaactgg cctcatatca
      421 acggcgtttt ttatgccatg gaggatcaag aacgcgttgt caatggattg caaggcgtcc
      481 tgcgcggcga atgctacttt acgcaaaagc ttgccagtta cctgattaca cattctggta
      541 actatcgtta taacagcacg gaatcagccc tccttactca tcgggaaaaa gagatcctga
      601 ataagctgcg tatcggcgcg tctaataacg agatcgctcg ttcgttgttc atcagcgaaa
      661 atacggttaa aacgcatctt tataatcttt tcaagaagat agccgtaaaa aaccggacac
      721 aagcggtttc ctgggcaaac gataacctca ggcgataaag ccatgaaacg ttatttacgc
      781 tggattgtgg cggcagaatt tctgttcgcc gcagggaatc ttcacgccgt tgaggtagaa
      841 gtcccgggat tgttaactga ccatactgtt tcatctattg gccatgattt ttaccgagcc
      901 tttagtgata aatgggaaag tgactatacg ggtaacttaa cgattaatga aaggcccagt
      961 gcacgatggg gaagctggat cacaataacg gtcaatcagg acgttatttt ccagactttt
     1021 ttattcccgt tgaaaagaga cttcgaaaaa actgtcgtct ttgcactgat tcaaactgaa
     1081 gaagcactaa atcgtcgcca gataaatcag gcgttattaa gtacgggcga tttggcgcat
     1141 gatgaattct aaataaaaaa ttgttcggag gctgcaatgc gtgtcaaaca tgcagtagtt
     1201 ctactcatgc ttatttcgcc attaagttgg gctggaacca tgactttcca gttccgtaat
     1261 ccaaactttg gtggtaaccc aaataatggc gcttttttat taaatagcgc tcaggcccaa
     1321 aactcttata aagatccgag ctataacgat gactttggta ttgaaacacc ctcagcgtta
     1381 gataacttta ctcaggccat ccagtcacaa attttaggtg ggctactgtc gaatattaat
     1441 accggtaaac cgggccgcat ggtgaccaac gattatattg ttgatattgc taaccgcgat
     1501 ggtcaattgc agttgaacgt gacagatcgt aaaaccggac aaacctcgac catccaggtt
     1561 tcgggtttac aaaataactc aaccgatttt taagccccag cttcataagg aaaataatca
     1621 tgcagcgctt atttcttttg gttgccgtca tgttactgag cggatgctta accgccccgc
     1681 ctaaagaagc cgccagaccg acattaatgc ctcgtgctca gagctacaaa gatttgaccc
     1741 atctgccagc gccgacgggt aagatctttg tttcggtata caacattcag gacgaaaccg
     1801 ggcaatttaa accctacccg gcaagtaact tctccactgc tgttacgcaa agcgccacgg
     1861 cgatgctggt cacggcattg aaagattctc gctggtttat accgctggag cgccagggct
     1921 tacaaaacct gcttaatgag cgcaagatta ttcgtgcggc acaagaaaac ggcacggttg
     1981 ccattaataa ccgaatcccg ctgcaatctt taactgcggc aaatatcatg gttgaaggtt
     2041 cgattatcgg ttatgaaagc aatgtcaaat ctggcggggt tggggcaaga tattttggga
     2101 tcggtgccga cacgcaatac cagctcgatc agattgccgt gaacctgcgc gtcgtcaatg
     2161 tgagtaccgg tgagatcctc tcctcggtga acaccagtaa gacgatactt tcctatgaag
     2221 ttcaggccgg ggttttccgc tttattgact accagcgctt gctggaaggg gaagtgggtt
     2281 acacctcgaa cgaacccgtt atgctgtgcc tgatgtcggc tatcgaaaca ggggtcattt
     2341 tcctgattaa tgatggtatt gaccgtggtc tgtgggattt gcagaacaaa gcagaacggc
     2401 agaatgacat tctggtgaaa taccgccata tgtcggttcc accggaatcc tgacagataa
     2461 aaatgccgga attaataatc cggcatttac tgtttaagcg aaagcgtgag tgcccctcac
     2521 gctttatttt tgatttcctg ctcacggaga gccgtctttg cactacgcgc cgccagccac
     2581 aggccgctgt ttttcattgc gtaaccaaat atcagcccaa gcgccagcga aggtaacacc
     2641 agtttccagt caccctgccc ggcaaacgtc gcgcatgcgc ctataaacgt ccccggaaca
     2701 aatgaaagca gcagctgttt ggcctgaata cacatcagaa acgcgacaat accggttatg
     2761 acataactga gaatttccag atgtggcgcc agtgcactac cgtaaataat gaccatcgcc
     2821 cacaccacgc cactaagcag agttgcagcg gagatcgcca gccctttcag cccaccttgc
     2881 gggcaggcaa agtaggccgt acagcccagg aagcccgccc agcttagtaa gcccagggaa
     2941 acagccaccc atccccagat accggagaga atgcctgttg tgattgcaat agagagaagt
     3001 atgttcatgc cgcgcatgat agcagaaaac gcggccatga atgagatcga gcacacattt
     3061 tatgcaacag caaattatct gttgcagtta tgagtgacgc atatcacatt tactcttcag
     3121 aatcttcttc cagctcgtcc cacattgcac taatggcatc gcgggttagc ggtgccatgg
     3181 tgcgccagaa aggcgtggtt gcatgggcct ccactttgcc aaggaacgca ccacaccagg
     3241 gtaacagata ctcactgaac agtgtttcca gtgcttcgct ctcatcttcc gttgactgat
     3301 cttccagcca ggaagccgca agcagcaatg tgccgatgtg atcggcaggc gtatccgcta
     3361 atggcatccc tcgttcagaa agaaaagcgc gcacttccgc ttccgttgcg tcctcaaccc
     3421 atgcgctacg atatggcggc acggcacatt catcgccgat aaacaacgca ttgtaatcgg
     3481 cagagacttg cgtcatatca cagcttttct gcaaacgtgt cagcaactca tcctgctcca
     3541 gtggccagtt cgcagccagt ttcccctcac gaatcagggt aaacagcggc accagtaaag
     3601 gatcttgcgg ttggcggtaa tagagcgaac ccagcacacg acagaggata gaaaactcgt
     3661 tcattaatat atttccatta ctagtaatta aaggtctgca aattccgcaa tcggtgccat
     3721 accgcgagat tcaaggaagt tcagtaagcg gcgcggagaa acattcaaaa tgcgctctgg
     3781 cggaaaatct accgcgtcga ggattttaag acactcttca aattccccca tggtaaacgc
     3841 ggtgtgagaa tccgagccta atgccaccca acctccagca tcacgtaccg ctgcggctac
     3901 cgcacgacag ttgtcttcac tgcccttacg tgagtgtaaa aatgaggaat tattgatttc
     3961 cagcgccacc tgatgtttcg cggctgcttc agcaacggct ttaacatcta tctcatattt
     4021 cggatttccg ggatggctta ttatatgcac attgccgctg gcgatagtgg cgatcatcgc
     4081 ttgtgtattg gtcgctttgt catgtggtgc aaaaaccggc tcatgaaaac cggcaataat
     4141 cagatccagc gagtcaaaca ttttaccgct gcaatcaatt tcaccatcaa catttttaat
     4201 gttagcttcg atgccgcgca ggatccctac cccatcaacc actcgcggcc agatacgcat
     4261 gttaatgaag tgccagtgat gcggcgcatc ttccatgtcc gggccatgat cggtaatcgc
     4321 aaaaagttta atgccctttt gtttggcctg ggcaatgtaa tcactcaatg tgctatatgc
     4381 atgtgtgctg gcaacggtat gcatatgaag gtcgacggga tacataactc tctcctgtat
     4441 tcaattcccg ccaaggatag caggaatcct gatgctttat tagtagccgc gtgcgcggtc
     4501 gacttgcccg cagaccctct cccctttttc gagctgggca atagtgcgag aaatgtactc
     4561 cacagcttca gcgggacggg taatcgcggc gacatgtggt gttatcgtca cgcgtggatg
     4621 ttgccagagc ggactttcag gcggtaaggg ttcacgatta aaaacatcca acattgcgcc
     4681 tttaactttg ccgctatcca gcgccgcgag caggtcatct tccacgacat gaacaccacg
     4741 cgccaggttg aggagatacg cgccatccgg taatttttcg agtaattgtt gattaataat
     4801 gccgacggtt tcaggggtat tcggtaacaa attaatcaat acccgacatt ggctcagaaa
     4861 tgcagacagt tcttcccatc cggcaaagct ttgcacgcca ggccacgatt tacgggttcg
     4921 actccagcaa cgcagcggaa agcgccaggt ttgcagactc tgagcaactt tactgcccag
     4981 tacgcctgcg cccaaaatgc cgatggtaaa atcttcccga tgatattcag gcagcggttg
     5041 ccaatgcgaa ctattttgct ggatgcgata atcgtcaaaa cgtcgaaacc aatgcagcac
     5101 ctgactgaca gcatattcct gcatttgctc gcccataccg gtatcttcca ggcgaaaaag
     5161 tggaacagaa ggcttcagca tttcagggtg tgcctgtagc ttgctcaaaa tagaatcaac
     5221 accggccccg agtgcgaaca ccgctttaag atcgcgccct gccagcattt caacaggagg
     5281 atgccaggct aaagcataat cagcagagtc attatctccg cttttccatg ctctgactct
     5341 tgcctgagga atagctttgc gcagtgcctc tatccaccat tgggtatcga acgttgggtg
     5401 ataaaagatg atatccatac tgactcccga aaagcgttgt gcgaaattta ttcgcactta
     5461 tcgttatgat ctacaaagac caccagcata acaaatccgt ggtcggtggc caaaaaagca
     5521 gattttgctt attaaaactg catattgatt gaagtttgaa taaacgcgcg attttttaaa
     5581 aaagtttgtt gacctcaggt catgatttcc ctaaattagc gcccgttcca gcaagacagg
     5641 aacgacaatt tggtgaggtg tccgagtggc tgaaggagca cgcctggaaa gtgtgtatac
     5701 ggcaacgtat cgggggttcg aatccccccc tcaccgccag attataagcg catgttattg
     5761 tttaacatgc gcttttttat tttcccttcc agacagtatg ccatcgggta tgccatctga
     5821 aaccggttta tatgccccgc gatcatatct tatcggacaa tttccgatct ttgagcatac
     5881 tggcaaagct atactgccag catatcagga tagtgttttg cgataatatc attcagtctg
     5941 tcaaccagtt ctggttttct gaacgtgata tgcgcggagc ccttcttaaa gtatctgatg
     6001 ctgaacatct catcttcata gctctcttta ccctgaacag actgaatgtg atcatccaga
     6061 cgaagggtga tgtttccccg gttgtcgggg attggtttac cgctgaacag gtgcaacatt
     6121 ctttccagat cggcgagtcg gtcagcctgt tgtccggtga taagatgaaa tccccaccgg
     6181 tcccatctca ccagattgtt gacgataatt ttactgccaa atttacaggg gcaattggtt
     6241 ttgtaattcc agttcagccc tctgaagacg ttgatcaccc ctcgctcaaa cacctcatcc
     6301 ttattctggt gcagttgttc aaatgtgctc agaatgttcg cttcgctgat ttccggaaaa
     6361 ttatcgtact ccagtgaccg gtaccatgta tcacgggcct gcgtatccat taatgacagc
     6421 atcccggaac gttgcatcag gtcacgccag atttcgcgat caagattccg ggtaatggct
     6481 ttcattgctg tttcgggttt ctccatcagc cagcagccgc agcgaaaatc ctgtttcatg
     6541 gcccagtccg gggcagtttt acctccgatg ctttccgtca gcaccgagat ttcagccagt
     6601 tgtcggataa gtgtgtcgat ttgcaacagt gtactctttc gtacagccag aatgtggcga
     6661 atgctggttg agcagataac gtccgtatgc ccgttgacca cttccggacg ggagattgtg
     6721 atatctgaca taatcgatat tcctttttgc agataaatga aaacgctagc cctgtgcagg
     6781 ctggcgtcag aggaatgaag tctggtgagt gagcttcacc ggttctggtg cagaagctgc
     6841 agatgacgca gggtacggac atcctcccgg gcctgttcga tacagggcaa cagtgctgct
     6901 gaatccgttt tatcctcatc gttgtcaaag ataattcccg atccgcagtc gatattgtcc
     6961 tgcagccacg caatcagaat atccagcgct gtttccgtgg ttaatgattt catgttgttc
     7021 ctggttacca gtcgaaagtg ggtaaacccg gcaggcattc agcgctggca tccagatgaa
     7081 tgagactgac gccataacgc cggatgagtg tgacgaccag acgacggaac gtttttgaca
     7141 gacccaggcg tttaagccgc agaaccgggt acgaccacgc atcggtacgc aacagatagc
     7201 cgttaccggt aaaatgaatc cattctgatt caccgaagtc actggtctgg tgtgacagcg
     7261 agtacagcca ggcgttgtcc gtttccgtga tatgtgcggt actgcagcgt atgccggtaa
     7321 gggtagcaaa tggtggcgtg cattctgctg aggtctggtc agattcatca acaacacgaa
     7381 gggagtagcc gttatcgcag accatgttaa tcagttcagc gagctcaata ccatcgacgt
     7441 caacgacaat gcgccccatg ttcagtgcct gtacgttaac gctgtcggct tccagtgtca
     7501 gggccagttt catcatttcg cctccggata cttacccagg gtaatgttat ttaccgttct
     7561 gtaattgtcg cgggtcatca ggccggtcgc ccggcgagcc cggaggatat caatactgtt
     7621 tattaactga gagcggggac aggtgctgaa tcccggctgg tcggtacgca ccagcgcgta
     7681 tttttccacg agaaaattaa ctgcatcaca cagtgaaatg cctgcctcaa tatgctgctc
     7741 aatcacacgt tcatcggcaa acggtgtgtc attcagtgtg aggccataat gctggtccag
     7801 cagtcggatg agcagtgtct gccagatggt gacgggagac gggcagcgag atacctcccg
     7861 tacatgtgtg tcggataatg ttttcatctt aagttctctg taactggtag tgctaaaggt
     7921 gagagcttcg gggttatgag gtggttgcgg gtgctggtgt cggataaaca gccagataaa
     7981 cgtaaccaca ggagccgagg gtgtcggctt cgcaggtcag cccttttgca ttcagcgtga
     8041 cggtatgctg atatcgggga ttcagttcac cgctggtgag catgagttcc agttgtttca
     8101 tcagcagcgg aaaggcctgg tccagatggt atgcatctgc gtcgctgaag cggcctctga
     8161 taccggcgcg gtcggcaagg taatgcaggc ggttaccctc ttgcaccaga cgagcgccaa
     8221 aacagggcct cactgtgcag ggaagccccc accaggggcg gtcgtgattg tcatcgggat
     8281 gagttatccc ggagagtttg tctgacacga taaaatcctc tctgaaaagt ggttaagaat
     8341 ggtttaacga tgacaggtca gccggcgcgc tgaccggttt cggtgaagat gtatgcattg
     8401 atgagaatgg cctcatccac agcggcgtct gaggtcatct ggtcgtactc gtttcccggg
     8461 tgacggaaca tcagttcagg gtgacactca tcagccggac atacgggcca aaactgtcct
     8521 tacggcgttc tgcaaacacg gcaagaacgc cgggaatatc ctgcacttca cgaccagtat
     8581 acgcttcagc actgccgtgc cagcgatact taccggtaca gaacggaaaa agacgggatg
     8641 caggatgctg ttggtgaata cgcatggctt caccacgggt gatgattctc atgatgtgat
     8701 acctctgaag acagaagata aaagtgaaaa caggtgtgat gtggttgtga cgggttaaag
     8761 cagaccatgt tcggcaaagg agaaaacctg gttgccaccg actatcagat ggtccggcac
     8821 ccggatatcc accaggccca gtgcctgtac cagacgttcg gtgataagcc ggtctgcctt
     8881 actgggtgtg acttcaccgg acgggtgatt gtgcgccagc accacggcag cggcattgtg
     8941 gtacagggcg cgtttaatca cttcccgggg atgaacttcc gtacggttga tggtgccggt
     9001 gaagagggtt tcaccggcaa tcagctgatt ctggttgttc agatacagca cccggaactc
     9061 ttcccgctcc agtcccgcca tgttcagaat cagccattcc cgtgccgcac gggtggaggt
     9121 gaaggccacg ccgggttcat gaagatggcg gtccagggtt tgcagggccc gcagaatgag
     9181 actgcgctcg ccgggcgtca tctctccggg cagaaaggaa agctgctgca ttgtccctgc
     9241 ctccattcag tcaatgatgc gcataatggc gctgcattcc ggatgctgca gggcgtaatc
     9301 ccgcagccgg taatagtgga ccgtcatggc ataacactcc gtacgacagg catgatgact
     9361 gtacgtcatc agacaggcag caataccggc ggcttccggg ctcatttcag cgcggttgcc
     9421 gttcatggca ttgaacagta cccatgtttc gtcatcatcg tcatccggtt cgggtgccat
     9481 aaatgccccg ccgttgttca gggtgtacag attccagata ccaccgcagt agtcttcgca
     9541 aagacggtcc atccagccga agacacgggg ctccagggtc acccactgtg gaatgaggcc
     9601 aaaatgctgc ggccagaagc tgatgcgctg ttcatcaggg acgggagtgg caaccagctg
     9661 aggtgggtta ttccctgatg cagtggttac ggaaacagaa ggtgtggtgg aattatgcga
     9721 aacggttgtc atgaggttat tccttataaa aagtaaatga atggaagaag ccccggggca
     9781 ggaacagatg tgagtcagga ctgcactttc agtgaaacgg catcagcgca tgctctccag
     9841 cagcgtttct gccatcaccc acaatgcgcg attgagctta atgtcggtat caatgctgtg
     9901 aatggcccgg gtatggatac gttttccttt tgcactgcga ccggaaatcc cgcctttcag
     9961 catattctcc tggatggtct gataagcact ccacaggtcc ttaccgtaat cctcccggcg
    10021 tcgtggtgtc agaatgtcgg cggtggtgac gggctgatgt tcgtcaccat aacggtaagt
    10081 cagtgccgcc tgtgccagcg cctggcgtgc cggtggcggc aggaccagcg actgcatggc
    10141 atcacgcttc tcctctatcc ggtcaaaaac gcccaccacc tcgtaagccc cttcaatgac
    10201 tctgtccacc acatttcccc ggtgtggaac acgcaattct cccagagact gaccgcagac
    10261 cagtccattg gtacaaatgg cacgaaaata tccgggtaac atctggtagc tggatgaacc
    10321 gtcatgggag ttgagcagaa taatttcagg gacatgctga ccggttatct gtccggcccg
    10381 ccgcagacgc agcatgtgtt ttgtgtattc ccggcggctc tggtcgcgca cgcgggtctg
    10441 gcaggcaaag aacggctgaa agccttcctg ctgcagattt tccaggacag tgatagtggg
    10501 aatgtacgca tagtgttcac tgcgagaggt atgtcggttt tccccaaaaa tactcggtac
    10561 atggcgcatc agttcttcgt gtgtcagcgg acgatcacga cgtatctggt tcgcataacc
    10621 aaaacgactg gctaatctca tgatttgctc cttatgaatg attaagcgat ataaatgaaa
    10681 aagccgcgtc tcccggagaa gacgcggcct gatgggtgaa atgaatgagg tttattgtct
    10741 gagaagccct tcactggcga accacgtatt aagctgtatt ccggcatcgc cagtgcaact
    10801 gaccttcagc attgcggata acccg
//
LOCUS       DBFIFI010000148        10824 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.148, whole genome shotgun sequence.
ACCESSION   DBFIFI010000148 DBFIFI010000000
VERSION     DBFIFI010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10824
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.148"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..756)
                     /gene="aroD"
                     /locus_tag="N7B39_003537"
     CDS             complement(<1..756)
                     /gene="aroD"
                     /locus_tag="N7B39_003537"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HGU2423451.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLALLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(787..1653)
                     /gene="ydiB"
                     /locus_tag="N7B39_003538"
     CDS             complement(787..1653)
                     /gene="ydiB"
                     /locus_tag="N7B39_003538"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HGU2423452.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDKF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENKSLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1665..2930)
                     /locus_tag="N7B39_003539"
     CDS             complement(1665..2930)
                     /locus_tag="N7B39_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2423453.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(3157..4371)
                     /locus_tag="N7B39_003540"
     CDS             complement(3157..4371)
                     /locus_tag="N7B39_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2423454.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQSNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFSFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            complement(4471..4827)
                     /locus_tag="N7B39_003541"
     CDS             complement(4471..4827)
                     /locus_tag="N7B39_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HGU2423455.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGRCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4963..5070)
                     /gene="rprA"
                     /locus_tag="N7B39_003542"
     ncRNA           complement(4963..5070)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="N7B39_003542"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5256..6368)
                     /gene="ydiK"
                     /locus_tag="N7B39_003543"
     CDS             complement(5256..6368)
                     /gene="ydiK"
                     /locus_tag="N7B39_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HGU2423456.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIQKPNK"
     gene            6757..9813
                     /locus_tag="N7B39_003544"
     CDS             6757..9813
                     /locus_tag="N7B39_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HGU2423457.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGLRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCARLDELIASHQAGVIGWQMCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLESLNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9810..10220
                     /gene="menI"
                     /locus_tag="N7B39_003545"
     CDS             9810..10220
                     /gene="menI"
                     /locus_tag="N7B39_003545"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HGU2423458.1"
                     /translation="MIWKRKIPLEALNAMGEGNMVGLLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEISDEKGRLCCSSRLTTAIL"
     gene            10320..10508
                     /locus_tag="N7B39_003546"
     CDS             10320..10508
                     /locus_tag="N7B39_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HGU2423459.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
BASE COUNT         2912 a         2630 c         2662 g         2620 t
ORIGIN      
        1 tgcctgatgt aaaatagtta atactgtgcg caaatcattt accgagattt gccctggcgc
       61 agaggctttt tttaccgcac caaaagttgc cgccgagcca aatacttcac cagccagacg
      121 agaaattacg ccagtttttg ccattgacat cgtgatgatt ggacgatcgg catactgctc
      181 ctgcatctcc agggtcgcgg caagcaacgc cagcacatcg ctggtacttt gcggcatcag
      241 cgcaatctta ggaatatcgg cgtcgaagga ctgcattttg cgcagacggg caatgatttc
      301 ttcggcttcc ggcgttttat ggaagtcatg gttggacatg acaactttca catcatgcgc
      361 gtgggcgtag gcgacggttt ctttaacctg atcatcgccg gtaaataact ccagatcgat
      421 catatcaacc aggccgctgt cgatggctgc acgattgaga gcaatataag cctcggtgga
      481 aatcgcctgc tcgccgcctt ctttggcact gcggaaggta aacagcagcg gtttttctgg
      541 catggtttca cggagaattt ttgccgccgc catgacagac tccacattgg agaggtcggc
      601 aaagtggtcc acacgccatt ccagaatatc aaagtccgct tcacgatagg cgagagcttc
      661 ggatttcact cgggcgatat ctttcgccat cagcgagacg atgattttag gtgcgcccgt
      721 accaatgacg agatctttta cagttacggt tttcattttt taccctttct gcacgcggtc
      781 agcctgtcag gcaccgaacc ccatgacctg tttaacatat tccagaggga aatctttgcc
      841 agtccataac gtgaactgtt cagccccttg ccacaacaac atgccgtatc catcaatcgt
      901 tttgcaacca gcttgttgcg cctgctgcaa taacttcgtc atatgcgggt tatacacgca
      961 ttcagtgacc agcagtcccg gatgtaacag actgatatca ttaaccaatg atttattctc
     1021 aaggggtttc atacccactt ttgtgccatt ggttaaaatg tcggcggaag ccagggcttc
     1081 agcaaaggct tgctgatcgg cgagatcggt aaccgtgacg acacaatcgg tgttttcgtt
     1141 aacccgctgc gcgaaggcga gggctttatc gaagaactta tcccgacggt taaagagttt
     1201 aatttctttt aaaccttcaa ttgccccctg cgcgccaatc gccgttgagg cacctccggc
     1261 ccctaacagc accattgttt tgcctttgat atcaaaaccg ctctctttaa tggcgcgaat
     1321 atggcccgtg ccgtcggtgt tatagccgcg caggtagcca tcatcattaa cgatggtgtt
     1381 gatggcaccc accagtttgg ctgctggcgt taattcatca acatattcac acgccagttg
     1441 tttgttcggc attgatacac cagttccgcg catttttaga gcttttaatc cttcaattgc
     1501 tccgggaaag ctatcgttat ccacttcgaa ggccatatag gtaaatggca atcccgcttt
     1561 ttctaaggct ttattctgca tttcaggcga taaactgtgg cggataggat aagccatcaa
     1621 cccaatcaat tcgtattttg cggtaacatc cataattaac tcctttaacc tctatgctta
     1681 attgtgttta accgggtgct aaatttgcgc tcgccaaacc gcacatcatt ctcaggaata
     1741 ataaaaacgc ggtaatagcg gataaaaaca ataattgcgg taatcagggc cagcagcgcg
     1801 aaagtaaaat cgagaacaat gatatattgc aggccgatgt tcgacagata accggtaatc
     1861 agcggaataa caaagttagc cagtccaccc atcatcatat aaatactggt gactttggct
     1921 ttgctttttg ggaaaaactc tgacatgacc gaaacaccga gctgtaaaat gccgccagct
     1981 gctgagaaac cgataacaaa ggcgccggca ttacacacca gtggagaagg gtacaggtag
     2041 ataatggctg ctgttattgt tgccagtgca gagttaaata cattagccca gatgggccgg
     2101 accatttttt tcagtagtgc ggcaaaaata aagacacaga ccagcgagcc catactgtaa
     2161 taagagatgg tttttaatgc ctcagcttct gacataccag caaaagccat cgcatatttg
     2221 ggcatccaca ccacaatcac ataaaaggtc gagaatgcgg ctacaccgaa cagtaccgat
     2281 gaaacacctt ccagccagac taacggtttg ctgttcattt gcggtaattc attagctacg
     2341 ctggcgtcca ccaactggct ggggaatttg cttttcaaca gcatcagcgt gatcagtaca
     2401 aacagaatac ccggaataat cagcccatag ccgtaccaga tattattgag caacatatag
     2461 ctcaccagca ttgggtagaa catttgccca aatgacacca tcgctttaac cagtatgacc
     2521 gccgaaccag aggctttcgg aaagcattcc atgagcgcgg ggtagccacc cgtatccagc
     2581 gctgagttag cgatacctac gcacactgcc agaccgtagg cgagagttaa attcgggcaa
     2641 gcgggaatac caaagaagaa tagcagatac attattactg ccattaatat caccgcccga
     2701 cgaccaaact tatcggagat cacaccgaag aataaaatac tgatcaatcg ccccaaaccg
     2761 ataccggaaa ttaagtaggc aatgcccgcg ttgtcagtgg aaaacttttc cgccagagat
     2821 gacatatttt gggcaagcgt aataacacta atgccgtgca ggaagtagct gaagtaaata
     2881 caaagaacag ccaggataaa tggcgtgctg aaagccttat tttgagacat tttcacagct
     2941 ccttgctgaa agataatttc gttaccgtac gtattgttct ccgacggttt acataattac
     3001 tatactgaag atgattttcc ctgggtacat ggcataaagt ttatgtttcc aggaatattg
     3061 catgtcatag tttgtttttg atcgcgattt tccttagtta gcgcagaaag agttatttat
     3121 ccgtaagatc ttgcatgacc tgaaaagaat attgcattac ccacccggag cgacattttc
     3181 ctttagcgag tgatgccgcg tttttttgcg gctgcgtaag ccgataaaca gtgccagtaa
     3241 aaaaccgatg gcagcgatgg cggtatcgaa ccacataata tcggcaatac ttctttggga
     3301 cagatgagcc gtaatcaacg gaatagtaaa ggtcgcaata ctgcccgcac tgtaatagat
     3361 ccctgtagct ttacctttag cgtaagggaa acgttcagcc attaacgtca ggccaatttg
     3421 cacaacacct ccagcagaag taaaaccaat gacaaaagaa aatattatca ccacataaaa
     3481 tgtgggatgc aggcagacgg taaacagagc aataaatgag ataaaggtgt acagcatcag
     3541 taatgttgtt gggcgaacgg tattacgaat gagtggagcg gtaataaata cacaaagcag
     3601 cgaacccacg gtgtagatac tgagtagttt gatcgacata gtgtatgaca tgcctgcaac
     3661 aaattgtccg tactgtgcca gccactggct aaccagataa aacgttgcca ttgagatata
     3721 gccatataag gtataactgg ctaaatcgat aattgaacag cgatgttccg tagagctggt
     3781 ggttttcttt atgacaggta agcgacgacc cggatggggt gggaacgtac aacggtataa
     3841 aaacagagcg ttaataaaca taatgcctgc agcaatcata aaggaccaac cgaaccacag
     3901 ttcagcccac actaacaggc taatgattag cggtaataaa aattgtccgc tggaaacaaa
     3961 tgctttaatt aaaatattgg ctgtcccagg tgagcgtgga aaagcttcca tcaaactggg
     4021 ataagtgcct gcatcgagaa aactgtttgc cattcccgcc agaaagccaa aaacataagc
     4081 gataatgatg ttattggtct gcaggatgcc aaaaaagaag gccatatagc agcacatccc
     4141 gagcatgata aaagggcggc gaccaaagcg atcggataat aatcctgcaa aaagcaagac
     4201 acttaatcga ccaatgccca gcgatgagat aactatcgag acacccgcgg cattagactg
     4261 ccaaagtgtc tccagcgagg ccatattcag gctcatcaaa atgacgccca taccatgcac
     4321 caggtaatta aaatacaacc caagtgcggt agggtagtag ggatttttca taggttctac
     4381 ttagcaaagg tgctttaaaa agtgtagcga tgttgtgtca gtatccgttc attattgttg
     4441 taacaacgac aattcctttt tgtggagaaa ttaacaaagc cgctccagat cgtgtgcata
     4501 taattcagcg gctaccgatt gatagatctt ccagtcaata aaattgccat cagggtagac
     4561 ttgttgaaac gcggttaagt cggggaaaaa acgcatggtg ttgttgccga tgcggttatt
     4621 tattttctct atgatcgcat gaagatgttt ttttctttgc tgacgcatag ccaacagttg
     4681 ctcgactaca caatcaggaa tggcacacct gccattttcc cattgacgcc agctttcact
     4741 gtcaccggtc tgggcaatcc aggtcgcgca ttcatcaata gtcatggcaa aaatatggcg
     4801 gagtgcctgg agttcataag cgttcatcgt gtaatggctg catgaaataa acaataaaat
     4861 ataaataaca ttatcagcct gctgacggct tgaagagagt cacagtatct tgtgcaacat
     4921 tattggaaag agtgaggggc gaggtagcga agcggaaaaa tgttaaaaaa aagcccatcg
     4981 tgggagatgg gcaaagacta cacacagcaa ttcgttgttt cactcagggg atttccatgc
     5041 ttataaatca atatgttgat ttataaccgt gagctaatag taggcatacg gactttttgc
     5101 gtcgatcaga ttcgtctcat tagtttaaaa actgtaaaga atttgcgaca ggtactacca
     5161 gctgaatggc ggtaagcagg aaaatatggg gaagagcaag caaatatcaa ttagcggtgg
     5221 ttaacaatca ggcggcagag ccgcctgatg agaaattact tattcggttt ctggatttcg
     5281 ccaagttctt cgagaatttc ttccggttga tccgtcggcg gtgggacctc ttccacccac
     5341 gcggcaaaca gacgccagga aacggctaac agaaccggac caataaacag accgatcatc
     5401 ccgaaagcaa tcaaaccacc aataacgcca gagaggatca ggatcagcgg taaatcggca
     5461 cccatgcgaa ttaacattgg gcggatgacg ttatccagcg tgccaaccac accgctccac
     5521 actaacaata ccgttcccca ggtggtatcg ccagtccagt agagccagat aatcgccgga
     5581 atcagtaccg gcaacgggcc aagctggaca aggcaggaaa ggatcattaa caccgttagc
     5641 aaagttgcat aaggtacgcc ggatacggcg aggccgatac cgccaagcac tgcctgtact
     5701 aacgccgtca ccaccacacc cagcgccacc gcgcggatag cctgtgccgc cagcagtacg
     5761 gcggcatctc cgcgaacgcc tgccagacgg gtcgcaaaat ggcgaatgcc ttgtgccacc
     5821 tgttcaccgc gccagtacag cagggcactg aagagaagca tcagcgcaca atgcaccata
     5881 aagcgcccga tatgcgccgc ttgcccaacg aaccaggtag tggtggtgcc aatataaggg
     5941 cggactttcg ccatgatcgc cgtccccccc atatccagca agttgtgcca gcctgcatac
     6001 agcttcgcgc caatcaccgg aatggtatta agccacgcca gatcgggtaa cgtcatgtca
     6061 ccgctggaaa tggctttaat tagcgggccg ctgccgtcga cgatactgtt aaccagcaaa
     6121 gcaataggga tgataaacac catcactaat aacagcgtca tcaccagaac ggcgagagag
     6181 cggcggccaa acatgatctt ttgcaaacgt aacaataccg gccaggtggc gataaccacc
     6241 gtaccggccc atgcaaagcc gagaataaag ggttgaacaa tccacagaca tgccacaatc
     6301 atgatggcta aaaacagcac cgaaagcaga atttgtgcga catccctggg ctgacgaaca
     6361 tttaccataa ctacttttca ccttttttgt gcgtcaaatc gtcgacgcga cgtgaacacg
     6421 cgaaactcac ctgcataatg atgagcaatt tcagcggttt ttaacaggcc gattctgccc
     6481 ttaattctga tgggcgtgtt agaaaaaaat gtggtacaac aactcaggca acacgcaaac
     6541 gatttactcg tcgtatttca agttgcatat gctgcggcta cgttcgttca ccccagttac
     6601 ttactgttgt aagctcctgg agttcactcg cttgccgcct tcctgcaact cgaaatccat
     6661 agagtaaata aacatcacct ttctgtgcgc tattgcagcc gcctgaaaga acagtgtatc
     6721 gacctacatc acagaccgca ggaaagggtc aatataatga ttccacagat ttcccaggca
     6781 cccggcgtcg ttcaactggt gcttaatttt ttgcaagagc tggagcaaca aggttttacc
     6841 ggcgatacgg cgacaagtta tgctgatcgt ctgacaatgt cgaccgacaa cagtatttac
     6901 caacttctcc ccgatgcagt ggtatttccg cgttcaaccg cagatgtggc gctgatcgcc
     6961 cgtcttgccg cgcaggaacg ctattcatcg ctgatcttta ccccccgcgg cggcggcacc
     7021 ggcactaacg gtcaggcgct caaccagggt attattgttg atatgtcccg ccatatgaac
     7081 cgcatcatcg aaattaaccc tgaagagggc tgggtgcgcg ttgaggccgg ggtgataaaa
     7141 gaccaactca atcagtacct gaaaccgttc ggctactttt ttgcaccaga actttcgacc
     7201 agcaaccggg caacgctcgg cgggatgatc aataccgatg catccggtca gggatcgctg
     7261 gtctatggca aaacgtcaga tcacgtactt ggcctacgtg cggtattgtt ggggggcgat
     7321 attctcgata cgcaaccttt acccgtcgag ctggcggaaa cgctgggtaa atccaatacc
     7381 acaatcgggc gaatttataa cacggtttat caacgttgcc gtcagcaacg ccagttaatc
     7441 atcgacaact tccccaaact taaccgcttt cttaccggtt acgatctgcg tcatgtcttt
     7501 aacgatgaga tgaccgagtt cgatctgacg cgcattctga cgggttcaga agggacgctg
     7561 gcctttatta ccgaagcgcg gctggatatt acgcgcttgc ctaaagtgcg ccgtctggtg
     7621 aatgtcaaat atgactcttt tgactctgcg ctgcgtaacg cgccgtttat ggttgaggcg
     7681 cgggcgcttt cggtagagac ggtggactca aaagtgctga atctggcgcg ggaagatatt
     7741 gtctggcatt ccgtcagcga gttgattacc gatgtgcctg acaaagagat gctcgggctg
     7801 aacattgtgg aatttgctgg tgatgatgag gcgctgattg acgagcgggt aaatgcactc
     7861 tgtgcgcggt tggatgagct gatcgccagt catcaagcag gtgtcatcgg ctggcagatg
     7921 tgccgtgagc tggcgggggt tgaacgtatc tatgcaatgc gcaaaaaagc cgttggtctg
     7981 cttggcaatg ccaaaggtgc cgctaagcca attccgtttg ctgaggatac ctgcgtaccg
     8041 ccggaacatc tggccgatta tattgctgaa tttcgcgcgc tgctcgacag ccacggctta
     8101 agctacggta tgttcggtca cgtcgacgca ggtgtcttgc atgtccgtcc ggcactggat
     8161 atgtgcgatc cgcaacaaga gattttgatg aagcaaatct ctgatgacgt ggtggcgctg
     8221 actgcgaaat acggtggttt gttgtggggc gagcacggca aaggttttcg cgctgaatac
     8281 agcccggcgt ttttcggtga ggaacttttt gcagaactgc gcaaagtgaa agcggcattt
     8341 gacccgcata accgactcaa cccagggaag atttgcccgc cagaaggtct cgatgcgccg
     8401 atgatgaaag tggacgcggt gaagcgcggt acattcgatc ggcagatccc cattgcggta
     8461 cgccagcagt ggcgcggtgc gatggagtgt aacggcaacg gtttatgctt caactttgat
     8521 gcccgtagtc cgatgtgtcc gtcgatgaag atcacccaga accggattca ttcaccgaaa
     8581 gggcgcgcaa cgctggtgcg tgaatggctg cgtttgttgg cggatcgcgg cgttgatcca
     8641 ctcaaactgg aacaagaact gcctgaatcc ggcgtcagct tgcggacgtt aattgcccgc
     8701 acgcgcaata gctggcatgc gaataaaggc gaatatgact tctcacacga agtcaaagag
     8761 gcgatgtcgg gctgtctggc ctgtaaagct tgttcgaccc agtgccccat caaaattgat
     8821 gtgccggagt ttcgctctcg ttttctgcag ctctatcaca cccgttattt acgcccgctg
     8881 cgcgaccacc tcgtcgctac ggtcgagagc tacgcgccgc tgatggcacg cgcgccgaaa
     8941 acctttaact tcttcattaa ccagccgctg gtgcgcaaac tctcggaaaa acatatcggc
     9001 atggttgatt tgccgctgct gtcggtcccc tcgctacaac aacaaatggt ggggcatcgc
     9061 tcggcaaaca tgacgctgga acagcttgaa tctctcaatg cagagcagaa agcgcgcaca
     9121 gtgttggtgg tgcaggaccc ctttaccagc tattacgatg cgcaagtggt ggcggatttt
     9181 gtccgtctgg tcgaaaaatt aggtttccag cctgtgttac tgccattttc gccaaatggc
     9241 aaagcccagc atattaaagg ttttcttaat cgttttgcga agacggcgaa aaagacggcg
     9301 gatttcctca accgtatggc gaagctgggt atgccaatgg tgggcgtcga tccggcgctg
     9361 gtactttgtt atcgcgatga atataaactg gcgctgggcg aggagcgtgg cgagtttaac
     9421 gtcttactgg cgaatgaatg gctggcaagc gcacttgagt cacagccagt ggctacagtc
     9481 agcggtgaat catggtattt ctttggtcac tgtaccgaag ttaccgcctt gccgggtgcg
     9541 ccagcacaat gggccgcgat atttgcccgt tttggcgcga aactggaaaa tgtcagcgtg
     9601 ggttgctgcg gcatggcagg gacttacgga catgaagcga aaaaccataa aaattcgctc
     9661 gggatctatg agttatcctg gcatcaggct atgcagcgac tgccgcgtaa ccgctgtctg
     9721 gcgaccggat attcctgccg tagtcaggtc aaacgggttg aaggcacggg ggtacgccat
     9781 cctgtgcagg ctttactgga gattattaaa tgatatggaa acgaaaaatc cccctggaag
     9841 cactgaatgc catgggtgaa ggaaacatgg tggggttact ggatattcgc tttgaacata
     9901 ttggtgatga cacccttgaa gcgacaatgc cagtagactc gcggacaaag cagcctttcg
     9961 ggttgctaca tggaggtgca tccgtggtac tggccgaaag tatcggttcc gttgccggtt
    10021 atttatgtac cgaaggtgag caaaaagtgg ttggtctgga aatcaatgct aaccacgtcc
    10081 gctcggcacg agaagggcgg gtgcgcggcg tatgcaaacc gttgcatctc ggttcgcgtc
    10141 accaggtctg gcagattgaa atctccgatg agaaagggcg tttgtgctgt tcgtcacgat
    10201 tgacgaccgc cattttgtga cgttgtcacc agaaaagtgt gacgcatatt gcttttggtc
    10261 attatctgca cagtaaacgg tggtatactg tgcaggtctt gtctaaaagc gaggatgcta
    10321 tgtctactca acttgatccc acccagctgg ccattgaatt tttacgccgt gaccagagca
    10381 atctttcacc tgcccaatat cttaaacgtc tgaaacaact agaacttgaa tttgctgacc
    10441 tgcttactct ctcttctgct gaattaaaag aagaaatcta ctttgcctgg cgtctgggcg
    10501 tgcattgatc ctgacttaat gccgcaagtt cgccaaatcg acctggatta acctccgtcc
    10561 aggttcgatg accttcttaa tactgcttat ctacccgcct gatattgttc aaaaataata
    10621 ctaatcgcag tttgtgttaa aacggcggct tagctttatg agattattct tatcgcccct
    10681 tcaagagcta agccactgag agtgccggag ataagcgccg gatggggtag aaacccttaa
    10741 gcctgtgtcg cacagactta agggttttct tatttctgaa cgctgcactt ttttacctat
    10801 ctcttaaata gtcattttca atac
//
LOCUS       DBFIFI010000149        10755 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.149, whole genome shotgun sequence.
ACCESSION   DBFIFI010000149 DBFIFI010000000
VERSION     DBFIFI010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10755
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.149"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..521
                     /locus_tag="N7B39_003547"
     CDS             <1..521
                     /locus_tag="N7B39_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGU2423460.1"
                     /translation="SLANNGDFLNREYDAVIMRENFCAPWAEVEYLFEEEILPVCSGS
                     LLAMSDQKLSVAELLTELPLLHQSTRITGWEEWFALSGVSSPLVNNGPRFDLLSMLIA
                     AVRSNLGVALLPRFAIQHDLDSGDMVIPCDVPIRTGNRFIMTWQEEKSDSPHLQQFRE
                     WLLAKSVVPQEM"
     gene            complement(555..1157)
                     /gene="cobO"
                     /locus_tag="N7B39_003548"
     CDS             complement(555..1157)
                     /gene="cobO"
                     /locus_tag="N7B39_003548"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308783.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HGU2423461.1"
                     /translation="MEARISTERHVQRQQKLKEQVDTRVAAAIEKKGILIVFTGNGKG
                     KSTAAFGTVTRAVGHGKTVGVAQYIKGQWDNGEYNLLQPLGVEFHIMGTGFTWETQNR
                     QADIDAAKEVWSESKRMLADKRYDLVVLDELTYMLAYHYLDTEEVIASLQNRPAQQSV
                     IVTGRGCHSQILKMADTVSEIRPVKHAFDNGIQAQPGIDW"
     gene            complement(1167..2819)
                     /locus_tag="N7B39_003549"
     CDS             complement(1167..2819)
                     /locus_tag="N7B39_003549"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HGU2423462.1"
                     /translation="MSKPFIWQEPFLQNKDGTEYTLISDQHITVTELDGEEVIKIAPE
                     ALTLLARQAFYEASFFLRSAHLQQVASILNDQQASSNDKYVALQLLRNAEVSAKGVLP
                     NCQDTGTATIVASKGQQIWTGGNDAEALSKGIYSTFQENNLRFSQNAPLDMYTEVNTQ
                     TNLPAQIDISAVAGDEYHFLCVNKGGGSANKAALYQEIKSLLQPEKLTAFLIEKMKSL
                     GTAACPPYHIAFVVGGLSADQTLKIAKLASTKYYDNLPTSGNEQGQAFRDIELEKVLL
                     EASQQFGIGAQFGGKYFAHDIRVIRLPRHGGSCPIAMALSCSADRNIKAKINKHGIWL
                     EKLEHNPGQYIPASLREENHAQHVQLDLNRPLRDVMQDLARLPVGTRVSLSGPIVVAR
                     DIAHAKIKARLDSGEPMPEYLKHHIVYYAGPAKTPENMACGSLGPTTGGRMDGYIDTF
                     QAAGGSLVMLSKGNRSQQVTDACHKHGGFNLGSIGGAAALLAQEYVKSLRCLEYPELG
                     MEAVWMMEVENLPAFILVDDKGNNFFSQFEQQHRCASCPAGH"
     gene            complement(2927..4207)
                     /locus_tag="N7B39_003550"
     CDS             complement(2927..4207)
                     /locus_tag="N7B39_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308785.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HGU2423463.1"
                     /translation="MKKISLTTMILLALVLGMIIGVVLNNTASPETAKLYAQEISIFT
                     TIFLRLIKMIIAPLVVSTLVVGIAKMGDAKALGRIFSKTLFLFICASLLSIALGLITV
                     NFFMPGTGINFVAHGAETTGVVAAEPFTLKVFISHAFPTSIVDAMAHNEILQIVVFSI
                     FLGCSLTAIGEKGSAIVHALDSLAHAMLKLTGYVMLFAPLTVFAAISALIAERGLAVM
                     VSAGIFMGEFYFTMLLLWVLLIGLAIVYVGPCIRRLTRALSEPALLAFTTSSSEAAFP
                     GTLEKLEQFGVSPKIASFVLPIGYSFNLVGSMAYCSFATVFIAQACNIHLSIGEQITM
                     LLILMLTSKGMAGVPRASMVVIAATLNQFNIPEAGLILLMGVDPFLDMGRSATNVMSN
                     AMGAAMVSRWEGEHFGEGCRGKALKPNESNVALP"
     gene            complement(4368..4685)
                     /locus_tag="N7B39_003551"
     CDS             complement(4368..4685)
                     /locus_tag="N7B39_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4387 domain-containing protein"
                     /protein_id="HGU2423464.1"
                     /translation="MKQSIVSLAQVIRSKNAGPYELVLDILFKTKENYERVKSSGQLT
                     PELIARLYHVEPDFIHRIVWFDPSNAVKIVMPRDIISGNVGDNDVYGAQQHAPLLNMS
                     FDL"
     gene            complement(4682..6052)
                     /locus_tag="N7B39_003552"
     CDS             complement(4682..6052)
                     /locus_tag="N7B39_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HGU2423465.1"
                     /translation="MARSFKVLSPTAILGYGFPEESFRKAMAESPDLIAVDAGSSDPG
                     PHYLGAGKPFTDRAGVKRDLRYMITAGVQNNIPVVIGTAGGSGAAPHLEWCREIIHEI
                     AREEHLSFSMALIPADVDKAIVHQALDNGKITALDFVPPLTHDAIDESTYIVAQMGIE
                     PFQRALKERAQVVLGGRAYDPACFAALPIMQGFDEGLALHCGKILECAAIAATPGSGS
                     DCAMGIIDDNGFTLKTFNPKRKFTETSAAAHTLYEKSDPYFLPGPGGVLNLKGCTFKA
                     VNDGEVYVSGSKHEETPYALKLEGARRVGFRCLTIAGTRDPIMIAGIDKIIDEVKTSV
                     SRNLSLDDDSIRINFHLYGKNGVMGDHEPMQTAGHELGIVLDVVAPTQEIANSVCSLV
                     RSTMLHYGYENRIATAGNLAFPFSPSDIQGGPVYEFSIYHLIEANDALRFDFHIEQVT
                     PEGVQA"
     gene            complement(6056..7297)
                     /locus_tag="N7B39_003553"
     CDS             complement(6056..7297)
                     /locus_tag="N7B39_003553"
                     /EC_number="4.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050096 - methylaspartate ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0019670 - anaerobic glutamate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate ammonia-lyase"
                     /protein_id="HGU2423466.1"
                     /translation="MKIKQALFTAGYSSFYFDDQQAIKNGAGHDGFFYTGEPVTQGFN
                     AVRQAGECVSVQLILENGAVAVGDCTAVQYSGAGGRDPLFLAEHFIPFLNDHIKPLLV
                     GRDVDAFLPNARFFDKLRIDGNLLHTAVRYGLSQALLDATALATGRLKTEVVCDEWQL
                     PRVAESIPLFGQSGDDRYIAVDKMILKGIDVLPHALINNVEEKLGFKGEKLREYVRWL
                     SDRILSKRTSARYHPTLHIDVYGTIGLIFDMDPLRCAQYIASLEKEAQGLPLYIEGPV
                     DAGNKPDQIRLLTAITKELTRLGSGVKIVADEWCNTYQDIVDFTDAASCHMVQIKTPD
                     LGSIHNIVDAVLYCNSHSMEAYQGGTCNETDVSARTCVHVALAARPMPMLVKPGMGFD
                     EGLDIVFNEMNRTIALLQAKD"
     gene            complement(7297..8742)
                     /locus_tag="N7B39_003554"
     CDS             complement(7297..8742)
                     /locus_tag="N7B39_003554"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0019670 - anaerobic glutamate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit E"
                     /protein_id="HGU2423467.1"
                     /translation="MELRNKKLTHDEFMTERQQVLKTWETGKDVENFEDGVKYQQTIP
                     EHKRFSLALLKADKEGKTLSQPRAGVALMDEHIELLKTLQEECDLLPSTIDAYTRLNR
                     YEEAAVGIKKSIEAGTSKLNGLPVVNHGVAACRRLTETLQKPLQIRHGTPDARLLAEI
                     SMASGFTSYEGGGISYNIPYAKRVTLEKSIRDWQYCDRLMGMYEEHGIRINREPFGPL
                     TGTLIPPFISHSIAIIEGLLALEQGVKSITVGYGQVGSLTQDVAAIQSLRELAHEYFQ
                     SYGYTDYELSTVFHQWMGGFPEDESKAFAIISWGAAVAGMSGATKVITKSPHEAWGIP
                     TAAANIQGLKASRQMLNMVNEQKFPPCPAVELEIELIKSEVRAVLNKVFELGNGDIAR
                     GTVLAFEAGVLDVPFAPAACNAGKILPVRDNTGAIRVLEAGAVPLPKDILDLHHDYVA
                     ERARCEGRQPTFQMVVDDINAVSHSKLIGRP"
     gene            complement(8761..10149)
                     /gene="glmL"
                     /locus_tag="N7B39_003555"
     CDS             complement(8761..10149)
                     /gene="glmL"
                     /locus_tag="N7B39_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase accessory protein GlmL"
                     /protein_id="HGU2423468.1"
                     /translation="MQIVSVDIGSTWTKAALFTREGDALTLVNHVLTPTTTHHLAKGF
                     FSSLNQVLNVDNALPLLNSGEVALKYSSSAKGGLAVAAMGLVPSIPLETAKVTAHSAG
                     AKIAQYYAYKLNRRDIQALEETQPDILLFTGGTDGGEESYGLNNARVLAESKLDCAII
                     YAGNRDIQDEVQEILGHKDLTIVDNVLPDLDHPNPVAARQAICDIFLKRIVKGKGLDV
                     IVDKTGEEPMPTPWTVFELVKAISNVDHSWKEFMLIDMGGATTDVYSACANTLSPDTV
                     LHGVPEPFVKRTVEGDLGMRVSAVVVGESAKELVNVNFAQQPAQLDAFYHYLRHLVAH
                     PDYLPANAEEKYFDTVLAGLCVGYATERHAGTKKEVCTCVGNVDLQMGRDLTTVRKVV
                     GSGGWLSRASQFDMHHWLKYRELNDDGKRILLPTEFEYYRDSRGLLPLLANVARVDPL
                     AAARTSIQCLTL"
     gene            complement(10149..10598)
                     /gene="glmS"
                     /locus_tag="N7B39_003556"
     CDS             complement(10149..10598)
                     /gene="glmS"
                     /locus_tag="N7B39_003556"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit S"
                     /protein_id="HGU2423469.1"
                     /translation="MKKATLVIGVIGADCHAVGNKVLDRVFSNHDFRVINLGVMVSQD
                     EYIDAAIETGADAIVVSSIYGHGDIDCLGMRERCIERGLGDILLYVGGNLVVGKHDFA
                     DVETKFKEMGFDRVFAPSHDLEDVCQLMAHDINQRHDVDTRILEEAI"
BASE COUNT         2517 a         2735 c         2731 g         2772 t
ORIGIN      
        1 attctttagc taataacggc gattttctta atcgggagta cgatgctgtc attatgcgcg
       61 aaaatttttg cgcgccctgg gcggaagtag aatatctctt tgaagaggag attttgccgg
      121 tatgcagcgg cagtttgctt gcaatgtccg atcaaaaact gtcagtggca gaactgctta
      181 ctgagttacc acttctgcat caaagcaccc gtattactgg ctgggaagag tggtttgctt
      241 tatctggcgt tagcagtccg ctggtcaaca acggaccacg tttcgatttg ctttcaatgc
      301 tgattgccgc cgtgcgctct aatctcggcg tggcgctgtt accacgtttc gctattcaac
      361 atgatttgga tagtggcgat atggtgattc cctgtgatgt gcctattcgt accggtaatc
      421 gttttattat gacgtggcag gaagagaagt ctgactcacc gcacttgcag caatttcgtg
      481 aatggttgct ggcgaaatcg gtcgtaccgc aggagatgta aaacctgcgc ctggcgggat
      541 gccaggcgca gttattacca gtcgataccc ggctgggcct gaatgccgtt atcaaaagca
      601 tgtttgacgg ggcggatttc gctgaccgta tctgccattt ttaatatctg actgtggcaa
      661 ccgcgtccgg tgacaatgac actttgttgt gccgggcggt tttgcagaga agcgatcact
      721 tcttcggtat ccagataatg gtaagccagc atataggtca gctcatcaag caccactaag
      781 tcgtaacgct tgtcagccag catccgtttg ctttccgacc agacctcttt cgccgcgtcg
      841 atatccgctt gccggttctg tgtctcccag gtaaaaccgg ttcccataat atgaaactca
      901 acccccagcg gctggagaag gttgtattcg ccgttgtccc attgcccctt gatgtactgc
      961 gcaaccccca ccgttttgcc atgacccacc gcgcgcgtca cagtaccaaa cgcggcagtg
     1021 gatttccctt tgccgttgcc ggtgaaaaca atcaagatcc cttttttctc tatcgccgcc
     1081 gcgacgcgcg tatcgacctg ttccttcaat ttctgttggc gctgcacatg ccgttcagtt
     1141 gaaattcgcg cttccattca ttttccttaa tgtcccgcag ggcaactggc gcagcggtgt
     1201 tgctgctcaa actgactaaa gaaattattg cctttatcat ctaccaggat gaatgcgggc
     1261 agattttcca cttccatcat ccacaccgct tccataccca gctccgggta ttccaggcaa
     1321 cgcagacttt tgacatattc ttgcgccagt aacgcagctg caccgccaat acttcccaga
     1381 ttgaagccgc catgtttgtg acaggcatcg gtgacctgct ggctgcggtt acctttcgat
     1441 aacatcacta gactgccgcc agccgcctgg aaggtatcga tataaccgtc cattcgaccg
     1501 cccgtggttg gacccagtga accgcaggcc atgttctccg gtgttttcgc cgggccagcg
     1561 taatagacaa tatgatgctt caggtactcc ggcatcggtt cgccgctatc cagccgcgct
     1621 tttattttgg cgtgggcgat atcgcgcgcc actacgatgg gaccacttaa cgacacgcgg
     1681 gtgcctactg gtaatctggc gagatcctgc atcacgtcac gcaacggacg gtttagatcc
     1741 agttgtacat gttgcgcgtg attttcttcc cgcagtgagg ctggaatata ttgccccgga
     1801 ttgtgttcga gtttttccag ccagataccg tgtttgttaa tttttgcttt gatattgcga
     1861 tcggcagaac aggagagcgc catagctatc gggcaagagc caccgtgacg cggcagacga
     1921 atgacgcgaa tatcatgagc gaaatattta cccccaaatt gcgcaccaat accaaattgc
     1981 tgactggcct cgagcaacac tttttccagc tcaatatcac ggaatgcttg tccctgctcg
     2041 tttccgctgg tgggcaaatt gtcgtaatat ttcgttgagg ccagcttggc aatttttaag
     2101 gtttgatcgg ctgaaagccc tccgacgaca aaggcgatgt ggtaaggcgg gcaggcagcg
     2161 gtgcccagcg atttcatttt ttcgattaaa aatgcggtca gtttttctgg ttgcaataag
     2221 gatttgattt cctgataaag cgcggcttta ttggcggagc cgcccccttt attaacacag
     2281 agaaaatggt actcatcacc agcgacagcg ctgatatcaa tctgcgccgg gagattggtt
     2341 tgcgtattga cttcggtata catatccagc ggcgcatttt gtgaaaagcg cagattattt
     2401 tcctggaaag tactgtaaat gcctttactt aaggcttcgg cgtcgttacc tccggtccat
     2461 atctgttgcc ctttactggc aacaatagtt gcggtgccgg tatcctgaca gttgggtaat
     2521 accccttttg ctgagacttc agcattacgt aataactgca acgcaacgta tttatcatta
     2581 ctgctggcct gctgatcgtt aagaatgctg gcgacctgtt gcaaatgagc ggaacgtaag
     2641 aaaaaagagg cttcataaaa tgcctgccgg gcaagcagcg ttaatgcttc aggagcgatt
     2701 tttatcactt cttcgccgtc taattccgtt actgtaatat gttgatcgct aattaatgta
     2761 tattctgtac cgtctttatt ttgtagaaac ggttcttgcc agataaatgg tttggacata
     2821 tttcactttt caccttaaac gttagtgtta cttaattgcc gtctaacttc atgatccggc
     2881 ttataaaaat gcgtagtcat tggcttctga ttgcttaaga tccccctcag ggcagagcaa
     2941 cgttcgattc attgggtttt aatgctttac cccgacagcc ctcgccgaaa tgttcgcctt
     3001 cccaccgact caccatcgca gcgcccattg cgttgctcat gacgtttgtc gcggaacgcc
     3061 ccatatcaag gaacggatca acgcccatca gcaagatcag ccccgcttcc ggaatattga
     3121 actggttgag cgtggcggcg ataaccacca ttgaggcgcg tggtacgcca gccattcctt
     3181 tcgaggtcaa catcaggatc aacagcatgg tgatttgctc accgatggat aaatggatat
     3241 tgcaggcctg ggcgatgaaa actgtggcga aggagcagta ggccattgat ccaacgagat
     3301 taaatgagta gccaatgggt aagacaaagc tggcaatttt gggggaaacg ccaaattgct
     3361 ccagtttttc aagcgttccc ggaaaagccg cttcagaact ggatgtggta aatgccagca
     3421 gggcgggttc cgaaagggcg cgggtcaggc gtctgatgca ggggccgaca taaacgatgg
     3481 ccagaccgat aagcagtacc caaagtaata acatggtgaa ataaaattca cccataaaga
     3541 tcccggcgct caccataact gccagtcctc gttcagcaat caatgctgaa atagcagcga
     3601 atacggtcag gggagcgaag agcatgacgt agccagtgag ctttaacatg gcatgtgcca
     3661 gcgaatctaa ggcgtgaacg atggcgctgc ctttctcgcc aatcgccgtc aggctacagc
     3721 cgaggaaaat tgagaacacc acgatttgca aaatttcatt gtgcgccatg gcatcgacaa
     3781 tgctggtggg gaaagcatgc gaaataaata cttttagcgt aaagggttct gccgcgacca
     3841 ctccggtggt ttcggcaccg tgtgcaacaa aattaattcc tgtacctggc atgaagaaat
     3901 ttaccgttat caagcctaag gcaattgaca gcaatgaggc gcaaataaat aaaaagagtg
     3961 ttttagaaaa aatacgacca agggctttgg catcgcccat tttagcaata cctaccacca
     4021 gggtagagac cactaacgga gcgataatca tttttatcag tcgtaagaaa atcgtcgtga
     4081 atatcgatat ttcttgcgca tagagttttg cggtttccgg tgaagcagtg ttattgagca
     4141 ctacgccgat aatcattcca agtaccagcg ccaaaagaat catcgtggtt aaacttattt
     4201 tcttcatttt ttcctccatc aggtgcacgg cgataaatca gaatcaggag aagtagatgc
     4261 ctgaatagag attcaggcat ctacctgcta ttacaacctt acaaatagct gaagtgttga
     4321 actgcaataa gtgacgggat gtcagttggc taaaagtatt cggattttta caaatcgaaa
     4381 ctcatattta ataaaggtgc atgttgctgt gcaccataga catcattgtc acctacattc
     4441 ccggagataa tatctcgtgg catgacgatt tttactgcgt tagaaggatc aaaccagaca
     4501 atacgatgaa taaagtcagg ttcaacatga tataagcgtg caatcagttc tggggttaat
     4561 tgccctgatg atttaactcg ctcatagttt tctttcgttt taaataaaat atctaaaacc
     4621 agttcgtaag gtccggcatt tttcgaacga atgacttgcg ccagtgagac aattgactgt
     4681 ttcatgcctg aactccttct ggcgtcacct gttcaatatg gaaatcaaaa cgcagagcgt
     4741 cgttggcttc aatcaggtga tatatggaaa attcgtatac cggcccgccc tggatatcag
     4801 aaggggagaa cgggaacgcc agattacctg cggtagcgat gcggttttca tagccgtagt
     4861 gcagcatggt agagcgcacc agcgagcaaa cgctgttggc aatttcctgg gtcggtgcaa
     4921 ctacatcaag gacaatcccc agctcatgcc cggcagtttg catcggttca tggtctccca
     4981 tcaccccgtt tttgccgtac aggtggaaat tgatgcgaat gctgtcatcg tcgagcgaca
     5041 ggttacgcga aacgctggtt ttgacttcat cgatgatttt atcgatcccg gcgatcataa
     5101 tcgggtcgcg cgttccggcg atggtcagac agcggaagcc cacccgtcgt gcaccttcca
     5161 gtttcagggc atacggcgtt tcttcatgct tagaaccgct gacgtagact tcgccgtcat
     5221 ttactgcttt aaatgtgcag cctttcaggt tcaacacgcc gccaggtcct ggcaggaagt
     5281 agggatcgga tttctcatac agtgtgtgtg cagctgctga cgtttcggta aatttacgct
     5341 tcggattaaa tgttttcagc gtaaagccgt tgtcatcaat gatgcccatc gcacagtcag
     5401 agcctgagcc gggcgttgcg gcgattgccg cgcattcaag gattttccca caatgcagcg
     5461 ccagaccttc atcaaagcct tgcatgatcg ggagcgcagc aaagcaggcc gggtcgtaag
     5521 cgcgcccgcc cagcaccact tgcgcgcgtt ctttcagcgc ccgctggaag ggttcgatgc
     5581 ccatttgcgc gacgatatac gtactttcgt caatcgcgtc gtgggttaac ggcgggacaa
     5641 aatccagcgc cgtgattttg ccgttatcca gcgcctggtg gacgatggct ttgtcaacgt
     5701 ctgccgggat caacgccatc gagaacgaga ggtgctcttc ccgggcaatc tcatgaatta
     5761 tttctcggca ccactccagg tgtggtgcgg caccagaacc gccagccgtt ccgatcacca
     5821 ccggtatatt attttgcacg cctgcggtga tcatatagcg cagatcgcgc ttgacccctg
     5881 cgcgatcggt aaacggtttg cctgccccga ggtagtgggg gcctggatcg gaagagcctg
     5941 catcaacggc gatcagatcg ggcgattctg ccatcgcttt gcggaagctc tcttccggga
     6001 atccataccc gaggattgcc gttggcgata agactttaaa agaacgagcc atttgttaat
     6061 ccttagcctg caacagcgcg atagtacgat tcatttcgtt gaagacgata tcgaggcctt
     6121 cgtcaaagcc catccctggt tttaccagca taggcatggg gcgagcggca agggcgacgt
     6181 ggacacaggt gcgggcactg acatcagttt cattgcaggt gccgccctgg tacgcttcca
     6241 tgctgtggct gttgcagtaa agaaccgcat cgacgatgtt gtgaatactg cccagatccg
     6301 gggttttgat ttgcaccatg tgacaactgg cagcatcagt gaaatcaaca atatcctggt
     6361 aggtgttaca ccattcatcg gccacaattt tcacgccgga accgaggcgc gtcagctctt
     6421 tggtaatcgc ggtcagcagg cgaatttgat cgggcttgtt accggcatcg accggccctt
     6481 cgatgtagag cggcaggcct tgcgcttctt tttccaggct ggcgatgtat tgcgcacagc
     6541 gaagcggatc catatcgaag atcagaccga tagtgccgta tacatcgatg tgcagggtag
     6601 ggtggtagcg tgcgctggtg cgcttgctta gaatgcgatc cgacaaccag cggacatatt
     6661 cgcgcagttt ttcaccttta aagcccagct tctcttcgac gttattaatc agcgcatggg
     6721 gcagcacgtc gatgccttta aggatcatct tatcgacggc gatatatcga tcgtcgccgc
     6781 tctgaccaaa taatggaatg gattccgcca cgcgtggcaa ctgccattca tcacagacca
     6841 cttcagtttt cagacggccg gttgccagcg cggtagcatc aagcagcgcc tgtgataatc
     6901 cgtagcgcac ggcggtatgc agcaagttgc cgtcaatacg caatttgtcg aagaaacggg
     6961 cattcggcag gaaagcatcc acatcgcggc ctaccagtaa tggcttgata tggtcgttga
     7021 ggaacggaat aaagtgctct gcgaggaaca gtggatcgcg accgcctgcc ccggaatact
     7081 gtacggcagt acagtcacct acggcgaccg cgccgttttc cagaatcaac tgtaccgata
     7141 cgcactcccc ggcctgacgt acggcgttaa acccctgtgt tactggctcc ccggtataaa
     7201 aaaagccgtc atgacccgct ccgtttttta tcgcctgctg gtcatcgaaa tagaatgagg
     7261 agtagccagc ggtgaacaga gcctgtttta ttttcattat ggtcttccta ttaatttact
     7321 gtgggataca gcattgatgt catctacaac catctggaat gtcggttggc gtccttcaca
     7381 gcgtgcgcgt tccgcaacgt aatcgtggtg gaggtcgaga atatcttttg gcaatggaac
     7441 ggctccggct tccagtacgc gtatcgcgcc ggtgttgtca cggaccggaa gaattttgcc
     7501 cgcgttgcag gcggctggcg caaacggcac atccagtacg cccgcttcaa atgccagcac
     7561 cgttccgcgc gcaatatcac cattacccag ttcgaacact ttattcagta ccgcgcgaac
     7621 ctcgctctta atcagttcaa tttccagttc aaccgcaggg cacggcggga atttctgctc
     7681 attgaccatg ttgagcattt ggcgggaggc tttcagcccc tgaatgttgg cagcggcagt
     7741 cgggataccc caggcttcgt gcggactttt ggtgatgact ttggtggcac cagacatccc
     7801 cgcgacggct gcgccccagg agataatagc gaacgctttc gattcgtctt ccggaaagcc
     7861 gcccatccac tggtgaaaca cggtactcaa ttcatagtcg gtatagccat agctctgaaa
     7921 atattcgtgt gcaagttcac gcagagactg gatcgccgca acatcctgcg tcaggctgcc
     7981 gacctggcca taaccaacgg tgatagattt cacaccctgt tccagcgcca gcaagccttc
     8041 gattatcgcg atagagtgcg agataaacgg cgggatcagc gtacccgtta gtgggccaaa
     8101 cggttcgcgg ttgatgcgga tcccgtgttc ttcatacatc cccattaaac ggtcgcagta
     8161 ctgccagtcg cggatcgatt tttccagcgt tacgcgtttg gcatacggaa tgttgtagga
     8221 aatgccaccg ccttcataac tggtaaagcc gctggccatt gagatttcag ccagcagacg
     8281 cgcatctggc gtgccgtggc ggatctgtag cggtttttgc agggtttcgg tgaggcgacg
     8341 gcaagctgcg acaccatggt taactaccgg taaaccgttc agtttagagg ttccggcttc
     8401 gatggatttt ttaatcccta cagccgcttc ttcgtaccga ttcagacggg tatatgcatc
     8461 gatggtgctt ggcagcagat cgcactcttc ttgtagtgtt ttcagcagtt cgatgtgttc
     8521 gtccatcagc gccacacctg cgcgcggttg gctaagggtt ttaccttcct tgtctgcttt
     8581 cagcagcgca agagagaaac gtttatgctc agggatggtt tgttggtatt taacgccatc
     8641 ttcaaagttt tcaacatcct tgccggtttc ccaggttttc agtacctgtt gccgctcggt
     8701 catgaattcg tcatgggtta atttcttatt tcgaagttcc attgattgca gccttaattg
     8761 ttatagggtt aagcattgaa tactggtgcg cgcggcggcc agcggatcga ctctggcgac
     8821 gtttgccagc agtggtaata gcccccggga atcgcggtaa tattcaaatt cggtcggtaa
     8881 gagaatgcgt ttaccgtcgt cgtttaactc gcggtatttc aaccagtgat gcatatcgaa
     8941 ctgactggca cgcgagagcc atccgccgga tccgacgact ttacgcacgg ttgtcaggtc
     9001 acgtcccatc tgtaaatcga cattgccgac gcaggtgcag acctcttttt tggtgcccgc
     9061 atgtctttcg gtggcataac ccacacacag tccggccagg acggtatcga aatatttctc
     9121 ttctgcattt gcgggtaagt agtcgggatg agcgactaga tgacggaggt aatgataaaa
     9181 agcgtccagc tgtgcgggtt gctgagcaaa attcacattg actaactctt tcgcactttc
     9241 accaaccacc actgccgaga cgcgcattcc cagatcgcct tccaccgtcc gttttacgaa
     9301 gggttccggt acgccgtgta aaacggtgtc gggcgagagc gtgttggcac aggcggaata
     9361 aacatcggtg gttgcgccgc ccatgtcgat cagcatgaac tctttccagc tgtgatcgac
     9421 gttactgatt gccttgacca gttcaaagac cgtccatggt gtcggcattg gttcttcgcc
     9481 tgttttatca acaatgacat ccagcccttt ccctttaacg atgcgtttaa ggaatatgtc
     9541 gcaaatagcc tggcgtgcag ctacagggtt cggatggtca agatcgggca gaacgttgtc
     9601 gacaatggtc aggtctttat gcccgaggat ctcctgcacc tcgtcctgaa tatcccggtt
     9661 tccggcataa ataatggcgc agtcgagttt ggattctgcc agcacacggg cgttattcag
     9721 tccgtagctt tcttctccgc cgtcggtgcc gccggtgaac aagagaatat cgggctgcgt
     9781 ttcttccagt gcctggatat cacggcggtt cagcttgtag gcgtaatatt gcgcaatttt
     9841 agcacccgca gagtgagcgg ttactttcgc ggtttccagg gggatagaag gcaccaatcc
     9901 cattgcggca accgccagcc cacctttggc agaggaggaa tacttcaatg caacctcacc
     9961 gctgtttaac agggggaggg cattatctac gtttaacacc tggttcaggc tggagaaaaa
    10021 ccccttcgcc agatgatggg tggtggttgg ggtcagtacg tggttaacca acgttaacgc
    10081 gtccccctcc cgggtaaaga gggctgcttt ggtccacgtc gatccaatat cgacagagac
    10141 gatttgcatc agatagcctc ttcaagaata cgagtatcaa cgtcatgacg ttggttgata
    10201 tcgtgggcca tcagctggca aacatcttct aagtcatgac ttggggcaaa gacgcgatca
    10261 aatcccatct ctttgaattt ggtttccacg tcagcgaagt catgttttcc gaccaccagg
    10321 ttaccgccca catagagcag gatgtccccc agtccacgct cgatgcagcg ttcgcgcatg
    10381 cccagacagt cgatatcgcc atgcccatag atagaggaga cgacaatcgc gtcagcgcca
    10441 gtttcaatgg cagcatcaat atattcgtcc tggctgacca ttacgcccag attaatgact
    10501 ctgaagtcgt ggttgctaaa aacgcggtcc agaactttat tgcctactgc atggcagtcc
    10561 gcgccaataa cgccaatcac aagtgttgct ttcttcatgg gtaatccttt gtaagacaga
    10621 gagtaaaata agttacacac gtcgtattta tggccgttca taacggcaca ggtttaatat
    10681 cacaggagtg tctttatctg accgtaatgt tgcaattgat ctattttcaa aatgtctttc
    10741 gggaaatgat tgttt
//
LOCUS       DBFIFI010000150        10754 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.150, whole genome shotgun sequence.
ACCESSION   DBFIFI010000150 DBFIFI010000000
VERSION     DBFIFI010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10754
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.150"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            147..578
                     /gene="osmC"
                     /locus_tag="N7B39_003557"
     CDS             147..578
                     /gene="osmC"
                     /locus_tag="N7B39_003557"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HGU2423470.1"
                     /translation="MTIHKKGQAHWESDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            complement(634..1560)
                     /locus_tag="N7B39_003558"
     CDS             complement(634..1560)
                     /locus_tag="N7B39_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HGU2423471.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMMFQDPLSSLNPRLPVWRI
                     ITEPLWIAKHSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGEAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     RERCPLATSGCEVRQSLTTRADGREIRCWRAL"
     gene            complement(1553..2539)
                     /locus_tag="N7B39_003559"
     CDS             complement(1553..2539)
                     /locus_tag="N7B39_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU2423472.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDTVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARDSGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            complement(2536..3432)
                     /gene="ddpC"
                     /locus_tag="N7B39_003560"
     CDS             complement(2536..3432)
                     /gene="ddpC"
                     /locus_tag="N7B39_003560"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HGU2423473.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQV
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            complement(3429..4451)
                     /locus_tag="N7B39_003561"
     CDS             complement(3429..4451)
                     /locus_tag="N7B39_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU2423474.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            complement(4453..6003)
                     /locus_tag="N7B39_003562"
     CDS             complement(4453..6003)
                     /locus_tag="N7B39_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGU2423475.1"
                     /translation="MKRSISFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASNDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            complement(6017..6598)
                     /gene="ddpX"
                     /locus_tag="N7B39_003563"
     CDS             complement(6017..6598)
                     /gene="ddpX"
                     /locus_tag="N7B39_003563"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HGU2423476.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFTCFISPGTQHVS"
     gene            complement(6856..9245)
                     /gene="dosP"
                     /locus_tag="N7B39_003564"
                     /gene_synonym="pdeO"
                     /pseudo
     CDS             complement(6856..9245)
                     /gene="dosP"
                     /locus_tag="N7B39_003564"
                     /gene_synonym="pdeO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
     gene            complement(9270..10652)
                     /gene="dosC"
                     /locus_tag="N7B39_003565"
     CDS             complement(9270..10652)
                     /gene="dosC"
                     /locus_tag="N7B39_003565"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HGU2423477.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPPIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
BASE COUNT         2658 a         2783 c         2687 g         2626 t
ORIGIN      
        1 atatatttcc ggttatctat tgtgggaatt taatttaagt gcagaagtaa tatttttgcc
       61 gtattttatt cggaatatcc tgcttatcct cgtgcggttt ctcacgtagt ctataatttc
      121 ctttttaagc ccacaggaga gcaacaatga caatccataa gaaaggtcag gcacactggg
      181 aaagcgatat caaacgcggg aagggaacag tatccaccga gagtggcgtg ctgaaccaac
      241 agccgtatgg atttaacacg cgttttgaag gcgaaaaagg aaccaaccct gaagaactga
      301 ttggcgcagc gcatgccgca tgtttctcaa tggcgctttc attaatgctg ggggaagcgg
      361 gattcacgcc aacatcgatt gataccaccg ccgatgtgtc gctggataaa gtggatgccg
      421 gttttgcgat tacgaaaatc gcactgaaga gtgaagttgc ggtgccgggt attgatgcct
      481 ctacctttga cggcatcatc cagaaagcaa aagcaggatg cccggtctct caggtactga
      541 aagcggaaat tacgctggat taccagttga aatcgtaaag cattgccgga tgacgcgtca
      601 ggcgcatgaa tgcctgatgc gttgttagca tctctacagc gcccgccagc aacggatctc
      661 acgtccgtca gcccgcgtcg ttaatgattg ccggacttcg catccgctgg ttgcaagggg
      721 gcaacgttcg cggaaaaaac agccctgtgg caacgtgcgg tttcctggca gatccgtttt
      781 acgtaatgcc cattcttcct ccagtggttt atcaatggcg gggagggaat ccagcaataa
      841 tcgggtgtat ggatgtgccg gagccgttag cacctgttgc gcttccccta attccacaat
      901 ctgcccgaga tacatcaccg ccacccgatc gctcatatga cgtatcaccg agacattgtg
      961 tgaaatcagc acataagtca gcccgtgatt ttcctgtagc gttaccagta aattgaggat
     1021 ctgcgcctgc acggagatat ccagcgcaga ggttggctca tcaagcacaa tcacgtcagg
     1081 ctgcgaagag agtgctctgg caatggcgat gcgttgccgc tgcccgccgg agaacgcatg
     1141 aggcaggcgg tcgagatact ccggacgaat acccacctgc acagccagct cctctgccaa
     1201 cgctcgccgc tgttgttcac tactatgctt agctatccag agcggttctg tgatgatgcg
     1261 ccacaccggt aagcgcggat taagcgaaga gagcgggtcc tgaaacatca tctgcataat
     1321 gcgttgtgag ccagaacgga tgtactgccc gtggctcggt tgcagcatac ccattaaaag
     1381 ctgtgcgagg gtgcttttgc cgcagcctga ctcgccgacg atccctaagg tttcaccacg
     1441 gcggatctgt aaatcaatac cattaatggc atgaacatgt tccgtagttt taccaagcca
     1501 gtttttacgc gccgggaaat tgatatggac gtcgcgtaac gttaataacg tgtcagacac
     1561 ttatgacctc ctgctgcgga taccaacagg cgcagcgctg gttgttgtca ccacacgctg
     1621 tcagcgccgg gacgttttca cactgtgcac ctgccgcata gcaacgatcg cgaaaagcgc
     1681 agccgtcagg caaatgggtg agatttggta ccgtccctgg aatggcgggt aatggctggc
     1741 gtggtactcc atgttctggt gcgcattgca gcaaaccaat ggtatacgga tgccggggat
     1801 gatggataac gtctgccgtc acgccgctct caatcacgct tccggcatac atcacgtaaa
     1861 cgctatcgca cagttgcgac accacggcca tatcatggct gatgaacagt accgcagttc
     1921 cactgtcgcg agctttatgt ttaagcaaac gcagtacctg caactgtacc gtgacgtcca
     1981 gcgccgtagt cggttcgtcg gcaataatca attgcggttc gcaggagaat gccagcgcaa
     2041 tcattacccg ctggcgcata ccacctgaaa gctcaaacgg atagcgcgac ataacttcca
     2101 cggtatccgg gatttgcatc tcttccagca gggcaatcgc tttagctctg gcttcccgac
     2161 gacttattgg ttgatgatgg cggatcacgt ccatcatctg aagacctatt cgacgtgtcg
     2221 gattgagggc ggtcatcggt tcctgaaaga tcatcgccac tcgtgcgccg cgccactgac
     2281 gaagctgttt ttcccgcgcg ttaagaacat cgtctcccag cagtgaaatc tgcccccgat
     2341 gtacgcaata actgcccgtc ggtagcagac gcataatcag cattgcggtg actgatttac
     2401 ctgagccgga ttctcccacc agaccgacaa tttcaccgcg gttaatctgc aaggacacat
     2461 tgttgagcgc gtgaacgtcg ccgttaaaac cggggaaact caaatgcagt tgttgaatgt
     2521 ccagaacggg ttgggtcatg actgctttcc tcctgctttc gggtcaagca gatcgcgaat
     2581 accatcacca aagagattaa acccgacggc ggtaagcaaa atcgctgccc ccggaaatgc
     2641 gcaataccac cactgatcga gtacatagtt gcgaccattt gctaccatcg cgccccattc
     2701 agcactcggt tgttgagcac ccaggccaat aaatcccaac gtggcggcca ttaaaatcgc
     2761 gctaccgata tccagcgata cctgcacgat cagcggcggt agggagttac gtaaaatatg
     2821 ccagttgatc agatgccaac gagacgcgcc aaaggttttc gccgcctgaa cataggtata
     2881 ctggcgtaca actaatgctt gcccccgcgc caggcgcaca taaaagggaa ttcgcacaat
     2941 agcaatagcc agcatggcgt taaacaaact cggcccgaga gcggcagcca gtgccattgt
     3001 cagtaccaac gaaggaatcg acagcataat gtccatgatg cgcatgatga tggcgtctgc
     3061 gcgtccgcca agcacaccgg atagacatcc gagtagcgaa ccaatcatac ccgcaatggc
     3121 aaccacgact aatccggcga gaattgattg ctgactgccg accagtacgc ggctgaacag
     3181 atcgcgtccc acttcatcgg tgccaaacca gtgcgccgca gaaggcggca aaaggcgggc
     3241 ggttaaatca atggcgttgg gatcatgcgt cgccagccac ggtgaaaaaa tcatcatcag
     3301 tagcattaat acaatgatga ctgcgccggt cacggtgagc ggactgcctt tcagcatcca
     3361 taccagtttt gcaccgttaa atcgcgtttg tttttgtggg cgtacggcgg acgtttcctc
     3421 gcttagcatc attcaccacc tccacgtccg atacgcggat caatccacaa atagagcaaa
     3481 tccaccacca ggttgaccag cacataagca aatgaaacca cgacggcaaa gcccatcact
     3541 gccgggaagt cgagcgcctg tattgatgtc actacccatg cccccattcc gggccaggca
     3601 aaaacggttt cggtgagcac tgcgccatac aacaaatcgc ccagcgccag ccccaatacg
     3661 gtaatcgaag ggatcaacgc attgggtagc gcataacata gaacgatata ccagccgggc
     3721 aagccgctgg cccgggcggt acgaatgtag tcttcactca attgttccag catcgctgag
     3781 cggatctggc gggcgacaat ccccaggtga acaaacgcca gcgttaatgc cggtaagatg
     3841 agatgttgca acgcattgaa gaagacttca ccgttgcctt caagcaacgc atcgagcaga
     3901 taaaagccgg taacgtgcgt tggtggatcc agccagtcat caagccttcc gccgccggga
     3961 agtatttgca gatgaccata aaacagcacg atgacgccca gcccaagcca gaacgcaggt
     4021 gtggagattc cggtaatcgc cattattcgc accagatgat ccagccagcg atttcgccag
     4081 actgcagaga gtatgcccag cgggatgcca atcaagagtg ccagcagcag ggcacaaaaa
     4141 gccagctcca gcgtcgccgg gaaaaatata cgcaactctt ccagcaccgg acgcccagta
     4201 cgaatggatg ttcccaggtc accatgaaac aggtcgctga cgtagcggta aaactgtaca
     4261 tacagtggct ggtccagtcc cagttgctgg cgaatatttt ccacgatagc atcgctggcg
     4321 cggtcacccg ccagtaaccg cgccggatcg ccggggatca gatgcgagat aataaacgta
     4381 atcacgcaga cgcccgccac cacgagcacc agcccccagc agcgttggcg taaaatactc
     4441 cagaaggtca cgttatttac tcatggtatt gatattgaag acctgttcca gcatgggatt
     4501 gaacacaaag cctttcacct ctttgttcat cgccagttgg tagtttttct ggaacaggta
     4561 cacataagca gcgtcatcaa tgacgatttt ctgtgcctgc tggtagtctc gggtacgctg
     4621 cgtctggtcg gtggtcgcaa gcgcattgcg cagtaactta tcgacctcac tgttttcata
     4681 gaacgagcgg ttacccggca gacctttttt gtctgactca aaccagtaat tcataaacat
     4741 atacgggtcg gcaaaatccg gactccagtt gccaatcgca atgtcgtaat cacctttacc
     4801 cactctgtcg cgcatggtgg cgttcgccag cttttccagc ttcacattga tgcccagctt
     4861 gttgaggctg gattgtgttg ccagagcgat cggttcccag ttcggatcgt tatcggagta
     4921 gagaaacgtc aggctggtgg gtttgctcgt cactttatcc cattcggctt tggctttcgt
     4981 ttcgtcatgg ttgtattgca ttgccgtcgc atcgtagccc cacatgcctt ccggaatcgg
     5041 gccgcgcatt tgttttccgt taccactcag aatgccgtta accatgccct gataatcggt
     5101 agaccaggaa atggcccgcc gcagatccgc ctgattaagt ggcgctttgc tgttattcag
     5161 atacagatag gtgacgcgca gtgacggata ctctgccaca ttgactttgt tttcctgctt
     5221 cagggcgttg agttgatcca ccggcagcgc atcggcaatg tcaatgtcgc cacgggagag
     5281 ctgcaggcga cgggaggcac tttcaccgat aatttttacc gatactcgct taaagttcgg
     5341 tttattgccg gggtaatgcg gatttggcac cagaactaat tgctgacctt tttgccagct
     5401 tttcagcata aatggtccgg aaccggcggt attttgcgcg aggaagccgc gggcatcatc
     5461 cgccgcatgt tcctttaata ccgccggatt gataatggat gcaccgtcat tcgccagcgt
     5521 gtagaggaac ggtgcaaatg gctggctaag ggtaaacttc accgtatgtt cgtcgggagc
     5581 atcaatcttt aaatctttgg gaaatgcttc tgctggcccc tggccgattt ttagtaaccg
     5641 ctcaaaagag agttttactg cttcggcagt gacaggtgtg ccatcggcaa atttagcatt
     5701 atctttcagg gtgaacgtcc actctttttg atcgttagac gctttccagc tacttgccag
     5761 atcgccttca acgtcggttg agcctttatc accgtccgtt ttgtactgaa ccagtcgctg
     5821 ataagacggg taggtcactg tccagtcgtt attatctatt gttaccgccg ggtcgagggt
     5881 ttgtggatcg gcggccttac caatcaccag catatctttc ggtacggcgg cgtgcgcaac
     5941 cgggaaagtt gtggcaagga cgagcgcgag caatgtggga cgaaacgata tcgatctctt
     6001 catggcagtt tcctgattaa ctgacgtgct gtgtgccagg ggatataaaa caggtgaatt
     6061 gatcagcgag cagagggtaa ctcgctgcct ggggtaattc gaagtgccac cattcgctgg
     6121 agataccgac aaaaccgcca ccagtcatta tcgcattcag caacaggcga ttgcgctgag
     6181 cggcgggcgg gacggaaggg tgataggcat gggagcgctc gtgcatttca tcgaaccctg
     6241 cgcccatatc caggatgttc ccgtgctcat cacgaagcgt caggtcgatc gccgtgccac
     6301 ggctgtgatt agaaccgacc gtcacatcaa caacatattg cgggtctggg caggcttgcc
     6361 acaacatcgc ctgtgcttgt tgtgggcgat acgcatcgta aatcaccagt tgtaagcctg
     6421 atagctgggc gatactgata cttttcgcca gcgcggtaat cgcatccttg tgtaacagac
     6481 aacgcgcttg ctggtaaata gctttacctg tgatgttatc agcgcaggcg tatttcaatt
     6541 cgatctccag atcagggaag atcacggcta aatcaaccag ttcggtggta tccgacataa
     6601 acggttcctg tataagacaa aaattgctgc gctttccgct tatgcagatc tcatgccatg
     6661 ccgggataag cgccagaatg ctggcttaaa gttatatttt ttaacttttg atcaacattt
     6721 gtgcagcgta gtgcagtttt ggtgcaagag gggaagttaa ggaaggaatc tcccggaatc
     6781 gtagctgaaa tcacagtatt taagtgacag tgtcacgtta aatgaaaacc cgcgagtgcg
     6841 ggagagagga atttgtcaga ttttcagcgg taacacgctg ctcatccagc ctggaatttc
     6901 ttcggcgggt agggggcggg aaaagaaata tccctgaata acgcgacagt ggatctttcg
     6961 tagcatctca aattgctctt tggtttcgac gccttccgcc acgacggtta aattgaggct
     7021 ttgcccaatg ctggtaatgg cttcaagtaa ggccaggatg cgtttttccg tcagacaacg
     7081 atcgacaaaa cttttgtcaa ttttgatttc cgttaccgga agactgacta agcgggataa
     7141 tccggaaaag cccgtgccaa aatcatctac cgataaaccc acgcccatat cacgcaggat
     7201 ctgaatgcgc ttaaagattt cggtatcgtg ttccatcatc atgctttccg tgatttctac
     7261 cgtcagctgg tggccgtcaa taccccaggc ttgcattgca tcagacacct gattaggcag
     7321 ctgattactg cgaaagtgca gcgccgacaa gttcacggat aacgccggga tatgaatatt
     7381 ctggctacgc cattctgcta actgacggca agcttccgcg atgacccagc gcccaatatt
     7441 ttcgatttca ccaatctctt ctgcgagagg aataaaccgt gaagggggca catgaccatg
     7501 ctggggatcg tgccagcgag caagggcttc gatgccgtag agttcacccg tttctgcgaa
     7561 gatttgcggc tggtaaacca gtttcagttg gttattgcta atcgcttctt tcagcgctgc
     7621 gcctaaaacc aaacgctctt ttaccatttc gttcatcgcc gggctgaaga actgccagcc
     7681 gttaccgcca ttcttgcgaa tataatccat cgcgttatgg gcagtggaga gcaggtaatc
     7741 gcgattttta cccacgtcgt agctgatgcc aatactcaag gtaagcggga aggggttatc
     7801 gtcaatcatt atcggcttgc tgaccacatt ccgtagctca tcggcgatct gggtaatgtt
     7861 actgacgtcg ttttccaggc tcacgaggac aaactgcgta ccttcgatac gacagagata
     7921 ctgatccggt ttgagttttt cacgaaagcg attgaccact tccagcaatg cctgatcggc
     7981 ccacgcatag ccaaggctat caatcacatc ctgaatatgg tcaacaccga tactggagag
     8041 acggctttgt cgaccaggtc atcgaggtaa ttgtgcaggt tattgcgatt tggcagaccg
     8101 gtcatcggat caaattggat gagttgttca atatgctgac ggcttttttc ctgttccagc
     8161 gccagcgcgg ccatatgctg gctgatatct gccacgcgtt cgataaaggc gctggtttct
     8221 gctcctgacg aggttttaat ttgcaggatc cccgcaggcg cgccatcacg ctgacgaatg
     8281 gtcgctgacc agctttgcgc attttgaact tctgcaccgt gcgaagatga cgcccagtgt
     8341 atcggcatcc cgttgcgcag tgcgaacagc gaaacatgcg attcgttgag aacagattcg
     8401 atgttacgac aaatgatttc ccccatttca tgaaatggcg ggctgctgca catggcggcg
     8461 agaatattgc cttcaagctg gcggatttgc cgttcttcgg tgatatccga gaaggtcatt
     8521 accaggttct gcagatgcgc gagcacgtca taaaccgggc tgatagaagc tttaatccag
     8581 attttttcac cggtgcgcgt caacagcaga aattcgtcct gatcgcgggc ggttttccaa
     8641 agcaactgtt gtaaacgaat gcggttatcg gcagggaatt caggaatgtt caggagtgta
     8701 tcaggctgca taccgctggc ttcgctaatg cagtaaccaa acatttcggt aaatgcgcga
     8761 ttgcactgca caatatggcg ttccggatcg aggacaatca ccggtcggtc gagatggtca
     8821 acggcaataa tcaattgcct ggtctgttct ttttgcgcca tttctacgct ggcatcccgt
     8881 accagcgcca ggtaataaac tttcccctcg gcgctcactt tcgatagcgc aaaacgggtc
     8941 cagattttac tgccgtcttt tttctccagc tgcagctccc gactcatccc ctcgacgcgc
     9001 gctttaccgc cttcacggtt gtgacgaatg tattcaggat gcgcaggacg caaatcccgc
     9061 ggaatcagca tatcaatgtt attgccaatg acttcttcac gtttgtaccc ccagagcttc
     9121 tctgcggcgg ggttgaaaaa catcacttca tcattttcgt taattaacac cgcacccatc
     9181 atattttgct caagggcggg gaaaaaaatg ccatcggcgg cattatccgc atcggttagc
     9241 ttcatgatta cctctgcatc ctggcgcatc taaagactgg ctttccagag ttcaacacgg
     9301 tttctacctc gtcttttggc gatatacaga gcttcatcgg ctatttgaat gaggcgctca
     9361 tagtcaggat gaccattaaa catggcggca ccgatggaaa gtgagagggc aatatcttcg
     9421 ccgtttgcgg ctttcagttt ggttttctcc acccgactgc gaatacgttc tgcggtacgt
     9481 aacgtttcgt tttcagaagc ttcagtcaaa acaatgataa attcatcgcc cccgtagcgg
     9541 aaaacataat cactactgcg gacgttgtca taaaaggcct gagagacttt acgcagaatt
     9601 tcatcgccag tgttatggcc ccacgtatcg ttgatctctt tgaatttatc aacgtcaata
     9661 atcagcactg acagcggtgt accggtccgg ttggcatggg caatttcgcg tttgaagata
     9721 gtcggcagga aacggcggtt aagtaatttc gtcagtacat ccataccgac ttcgtggcgc
     9781 gatacttctt caaacaattc acgcagcaag gtaataattt gcgatacggt atttcttatc
     9841 tgtaataaaa atttcacccg cagacttctg ttattcaaat tcctggtgtt acgcatggtt
     9901 tgattgaaaa taccgtcgaa atcctgaatc agacgagaga tatggcccac ttcagcaatg
     9961 ccgctgaaat aatgtcgacc tttatggtta aaccacaggc caaaatcagc ctggcttaaa
    10021 ggcaaactac tgcctaaatc agaatccagc aggattttat agataatatc tatttcccat
    10081 gaaagtattg aggctatttg ccgttctttt tcttcttcgg cgttttccag taacgagaag
    10141 atacgatagt tttcatcttc ttttgaggca ctactgtcac taaaggtaaa cgcgcgggtc
    10201 atcacttcca tagcgatatc aatactgtta atcgagaaat ggtagacctg aagtttttct
    10261 gcggcagaat aatccgaaga gaagattacc ggatagagga tctttttcag cacccgaaac
    10321 cccatctcga caatttctac cggaattcct atgcgggcat gcacttccgc gacggtatgc
    10381 tggatttgta ttagcctttc gacatcgtca acctgggcag aaagcacgtt aataatccag
    10441 cgttccatcg cacttttcaa ctgccgctca acttgttcat tactcaagaa ttcttcggca
    10501 tgcgggtcga tgcggacaat tcgataaaac tcgatactca gataatgagc atgcgcaacg
    10561 gcaatttccg cggctttagc acgaatgggc ggatctgcct gctcgacaag tccggtccac
    10621 tcatctttca ttcttttaaa atacatctcc ataattcaca cccttataag gctgggaaat
    10681 cagacggaat caaaatgaaa cgcgacgtgc gagatcgact aactgcacca tattctcctg
    10741 aaatatgaag atat
//
LOCUS       DBFIFI010000151        10690 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.151, whole genome shotgun sequence.
ACCESSION   DBFIFI010000151 DBFIFI010000000
VERSION     DBFIFI010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10690
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.151"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            162..1518
                     /gene="dcuC"
                     /locus_tag="N7B39_003566"
                     /pseudo
     CDS             162..1518
                     /gene="dcuC"
                     /locus_tag="N7B39_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
     gene            complement(1561..2058)
                     /gene="sspB"
                     /locus_tag="N7B39_003567"
     CDS             complement(1561..2058)
                     /gene="sspB"
                     /locus_tag="N7B39_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HGU2423478.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(2064..2702)
                     /gene="sspA"
                     /locus_tag="N7B39_003568"
     CDS             complement(2064..2702)
                     /gene="sspA"
                     /locus_tag="N7B39_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HGU2423479.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(3097..3489)
                     /gene="rpsI"
                     /locus_tag="N7B39_003569"
     CDS             complement(3097..3489)
                     /gene="rpsI"
                     /locus_tag="N7B39_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HGU2423480.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(3505..3933)
                     /gene="rplM"
                     /locus_tag="N7B39_003570"
     CDS             complement(3505..3933)
                     /gene="rplM"
                     /locus_tag="N7B39_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HGU2423481.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(4152..5279)
                     /gene="zapE"
                     /locus_tag="N7B39_003571"
     CDS             complement(4152..5279)
                     /gene="zapE"
                     /locus_tag="N7B39_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HGU2423482.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLHDETRAQMDKLWLALAGGKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            5473..5871
                     /gene="zapG"
                     /locus_tag="N7B39_003572"
     CDS             5473..5871
                     /gene="zapG"
                     /locus_tag="N7B39_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HGU2423483.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            6025..7392
                     /gene="degQ"
                     /locus_tag="N7B39_003573"
     CDS             6025..7392
                     /gene="degQ"
                     /locus_tag="N7B39_003573"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HGU2423484.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKTGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            7482..8549
                     /gene="degS"
                     /locus_tag="N7B39_003574"
     CDS             7482..8549
                     /gene="degS"
                     /locus_tag="N7B39_003574"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HGU2423485.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(8613..9551)
                     /gene="mdh"
                     /locus_tag="N7B39_003575"
     CDS             complement(8613..9551)
                     /gene="mdh"
                     /locus_tag="N7B39_003575"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HGU2423486.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            9986..10456
                     /gene="argR"
                     /locus_tag="N7B39_003576"
     CDS             9986..10456
                     /gene="argR"
                     /locus_tag="N7B39_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HGU2423487.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT         2662 a         2705 c         2638 g         2685 t
ORIGIN      
        1 caaaataatc ataagcgcat attttttaat gaaaaatcac ctcacctaca attaaaaaca
       61 cgacatccgc accataaata gacttgcaaa aaatataaca tcattgtttt caatctgccg
      121 tttatgggat tgaccctttt cttttgacac ggagttcaac aatgttcggc ataattatat
      181 ctgtcatcgt attaattacg atgggctatt tgatcctgaa aaactacaaa cctcaggtgg
      241 tgctggctgc aggtatcttc ctgatgatgt gcggtgtctg gttagggtta ggtggtgtac
      301 tcgatcccgc caaaagcagc ggctacttga tcgtcgatat ttataatgaa atcctgcgca
      361 tgctgtccaa ccgcattgcc ggattggggc tgtcgattat ggcggtgggc ggttatgccc
      421 gctacatgga gcgcacagga gccagtcgcg cgatggtgag cttgttaagc cgcccgttaa
      481 aactcattcg ctcgccgtat attattctgt cggcaactta cgtcatcggc caaatcatgg
      541 cgcagtttat taccagcgcc tccggcctgg gtatgttgct gatggtcacc ttatttccga
      601 cactggtgag tctgggagta agccgtctct ctgcggtggc ggttatcgca accacaatgt
      661 ccattgagtg ggggattctg gaaacgaact ccatttttgc agcccaggta gcgggaatga
      721 aaattgccac ttacttcttc cactaccagc ttccggtcgc ctcttgcgtc attatctcgg
      781 tggcgatctc ccactttttc gtgcaacgcg cttttgacaa aaaagataaa aatatcaatc
      841 acgaacaggc agagcaaaaa gctctcgata atgtcccgcc gctctattac gccattttac
      901 ctgtgatgcc gttaatcctg atgctcggct cgctattcct tgcccacatc gggctgatgc
      961 agtcagaact gcatctggtg gtggtgatgt tactgagttt gacgatgttt gttgagttct
     1021 tccgcaagca taacttgcgc gaaacaatgg acgatgtgca ggcgtttttt gacggcatgg
     1081 gtacgcagtt tgccaacgtg gtaacgctgg tggtcgcggg tgaaatattt gcgaaaggct
     1141 taacgacgat tggcactgtc gatgcggtta tcaggggtgc ggagcattct ggtctgggcg
     1201 gtattggcgt gatgattatt atggcgctgg tcattgccat ttgtgccatt gtgatgggct
     1261 ctggcaatgc gccgtttatg tcatttgcca gtcttattcc gaatatcgca gccggactac
     1321 atgtaccagc ggttgtaatg attatgccga tgcattttgc cacgacgcta gcgcgcggtt
     1381 tcgccgatta ctgcggtggt ggtcgttacg tcaggaattg caggcgtttc gccttttgcg
     1441 gtggtgaagc ggacagcgat ccccatggca gtcggtttcg tggtgaatat gattgccaca
     1501 atcacgctat tttattaagt cattaaaaga caaaacaggc cgccagggcc tgtttttgta
     1561 ttacttcaca acgcgtaatg ccggtcgacc accgcgtggt ggctgcggtg gttcatcgtc
     1621 aggatgagtg tcatcatcgt gatctggctt gtcgccatca ataaccgaca taacggtttc
     1681 gttgtctgcc gatgcctctt catcattcat gatgctggta tcttcatcgt aggcagcttc
     1741 aggctcaaac atcgtgcctg cgccattttc acgggcgtag atagccagca cggcagccag
     1801 cggcacagaa acctgacgcg gaatgccacc aaagcgcgcg ttaaagcgca cctcatcatt
     1861 cgccagttcc agattgccga cagcacgcgg cgcaatgttg agtacgattt gcccgtcacg
     1921 cgcatattcc ataggaacct gcacgccagg gagcgtcaca tccaccacca ggtgcggcgt
     1981 gagctggtta tccagcaacc actcatagaa tgcacgcagc agatagggac gacgtggtgt
     2041 tagctgtgac aaatccatac agattaactc cggcccagac gcatttcacg ttctgcttca
     2101 gttaaagaag caaggaaaga gtcacgctca aagacgcggg tcatatagcc tttcagctct
     2161 ttcgcacccg ggccgctgaa ctcgataccc agttgcggca gacgccacag cagcggagca
     2221 aggtagcaat cgaccaggct gaactcatcg ctcaggaagt acggcttctg accgaagacc
     2281 ggcgcaatcg ccagcagttc ttcgcgcagt tgcttacgtg cggcatctgc ttcagaagct
     2341 gaaccgttga tgatggtgtt catcagcgtg taccagtctt tttcgatgcg atgcatgtac
     2401 aaacggcttt caccgcgagc taccgggtaa acaggcatca gtggcggatg cgggaaacgc
     2461 tcatccagat attccataat gatgcgagat tcccacaggg tcagctcacg atccaccagg
     2521 gtcggaacgc tctgattcgg gttgaggtca atcagatcct gaggcggatt gtccttttcc
     2581 acgtgttcga tctcgaaact tacacctttc tcagccagca caatgcggac ctgatggcta
     2641 tagatgtcag taggaccgga aaacagcgtc attaccgaac gtttgttggc agcgacagcc
     2701 atgaaaacct ccaggtatag tcagaatttt tactgctacc agccaccagg tggccagtca
     2761 gaagttgtgt tacccagtaa ggaacgactc tctttgttcg aaaatcaaac aaaaaatgag
     2821 caatacccga catttgggca gaaaattgga tgatagttta ccagattttg cgacctttgt
     2881 ggtgagtcga tgccggaaat ggggaaaaag agatgcgctt tagtctgaaa tagttgactt
     2941 agtcccttat tggcgatgtg gtttttgttt tgcctgtctg tcagacggca gcaaaaagca
     3001 actttccatt ttttacgctg attcagaatt taaccataaa aaaacccgcc gaagcgggtt
     3061 ttttcgcaaa ttgttttctg ccggagcaga agccaattaa cgtttggaga actgcggacg
     3121 acgacgtgct ttacgcagac cgactttctt acgttcaacc tgacgagcgt cacgagtaac
     3181 gaagccagct ttacgcagtt cagaacgcag ggactcgtcg tattccatca gagcgcgggt
     3241 gataccgtga cggatcgcac cagcctgacc agagatacca ccacctttaa cggtgatgta
     3301 caggtccagt ttctcaacca tatcgaccag ttccagcggc tgacgaacta ccatgcgagc
     3361 agtttcacga ccgaagtact gttccagaga acgttggttg attacgattt taccgttgcc
     3421 cggtttgatg aaaacgcgag ctgcggaact tttgcggcga ccagtgccgt agtattgatt
     3481 ttcagccatt gcctataatc ccgattagat gtcaagaact tgcggttgct gtgccgcgtg
     3541 gttgtgctcg ttacccgcgt aaactttcag tttacggaac atagcacgac ccagcgggcc
     3601 ttttggcaac atgcctttaa ccgcgatttc aatcacacgc tcaggacggc gagcaatcat
     3661 ctcttcaaag gtcgcttgtt tgataccacc gatgtggccg gtgtggtgat agtacacttt
     3721 gtcagtacgc ttgttgccgg ttacagcaac tttgtcagcg ttcagaacga tgatgtaatc
     3781 accggtatct acgtgcggag tgtattccgc tttgtgctta ccgcgcaggc gacgagccag
     3841 ttcagtagcc agacggccca gagttttacc ggtcgcgtca acaacatacc agtcgcgttt
     3901 tacggtttct ggtttagctg taaaagtttt cattaaaagc ttacccaata aatagttaca
     3961 cgttggtgaa cacccaaacg tcttcaattg ttgaggttca cacgacaaag tccggcaaac
     4021 ctaccccttc gaatagccta tgccagcaca caaaaagttt tgggaaaaaa actttcttgt
     4081 aacgtggggt cgcaggatta tagagaagtc ggggtcaaag atcgacccct ttttgtgatt
     4141 tgtggcaggt tttaacccgc taaatgctcg cgcttcagat actcttcgct ttgcatctct
     4201 tgcagacgtg acaggcaacg ctggaactca aacttcagcc gatcgccctg ataaatttca
     4261 tacagcggca cttctgcact caccactaat ttgacatggc gctcgtaaaa ctcatccacc
     4321 agcgcaataa agcgccgcgc ttcgctctcc atcaaccgcg tcataactgg tacatcaaac
     4381 aacatgaccg tatgaaagag acgtgagagc gcaatatagt catgctgact gcgggcgtcg
     4441 acgcacagcg tagtaaaaga gaccgccagc gtctggttct cgacgcccat tgtcgctaat
     4501 ggccgatggt tgatttctaa cgtcggtgaa ttttctcgtt tcccccccgc cagcgccaac
     4561 catagtttat ccatttgcgc ccgggtttca tcgtggagtg gcgaaagcca cagatgcgcc
     4621 tgagtgagtg tacgcagacg ataatcaaca ccagcgtcca cgttcattac atcacaatgc
     4681 tgtttaatgg catcgattgc aggcagaaaa cgcgcacgtt gcaagccatt tcgataaagt
     4741 tcgtccggcg gaatatttga cgtcgctacc agggtaatac cgcgggcgaa cagggctttc
     4801 atcaggccgc caagtagcat ggcatcggta atatcagaga caaaaaattc gtcaaaacag
     4861 agcacgtcag tttctgcttt aaaacgatca gcaataattt ccagcggatc ggtctgcccc
     4921 tgtaaggcag ttagctcttc atgcacccgc agcataaaac ggtgaaagtg caggcgctgt
     4981 ttccgctctc ccggcaggct ttgatagaaa aggtccatca gccaggtttt cccgcgtcct
     5041 acaccgcccc acatatataa gccacgcact ggcgtatgct ttgtgtcttc ccgtttaccc
     5101 cacagcttac cgacccgcgc cattagccca ctcgtcctgg gggctggtgg cgtgctattg
     5161 atgagttcct gataaataat ttccaagcgg ctgacggcct ctttttgaac gtcgtcgggt
     5221 tgatggctgc cttcattgag cgccttcagg tattgcgatg ttggagtaac gctttgcatg
     5281 atcttattgt tattccttga ataatcggtg cgccgttgtt cacggttgac gaaaaaaagg
     5341 ccgttctaca ctacgcgata tgcagtcggg attccacttc tgtggaatta acggttatag
     5401 tggcataatc agccgcaggc atggagcctg aagccaacac cctacggaaa caaaagacaa
     5461 cgggagatgt tcatgacctg ggaatatgcg ctaattgggt tagtcgtcgg catcattatt
     5521 ggtgctgtgg ccatgcgttt tggtaatcgt aaactacgcc agcaacaggc gttgcagtac
     5581 gaactggaaa agaataaagc tgaactggac gagtatcgcg aagagctggt tagccacttt
     5641 gcccgcagcg cggaattact ggataccatg gcgcacgact atcgccagct gtatcagcac
     5701 atggcgaaaa gctccagcag cctgctgccg gaactgtctg ctgaagcaaa cccgttccgt
     5761 aatcgtctgg cagagtctga agccagcaac gatcaggcac cggtgcagat gcctcgcgac
     5821 tattctgaag gcgcatccgg cctgctgcgt actggcgcga agcgcgacta atttattttt
     5881 cgggcgcagc cattgcgccc tcctcttctc tccctccccg actatcattt aatctggtgt
     5941 ctcattgtta gccgtctgaa aattcaataa catcaaactg ttttgaatct cttttcttat
     6001 cattcaggta cgagagcagg aataatgaaa aaacaaaccc agctgttgag tgcattagcg
     6061 ttaagtgtcg ggttaactct ctcggcgtca tttcaggccg ttgcgtcgat cccaggccag
     6121 gttgccgatc aggcccccct ccccagtctg gctccaatgc tggaaaaagt gcttccggca
     6181 gtggtgagcg tacgggtgga aggaacggcc agtcagggac agaaaatccc ggaagaattc
     6241 aaaaagtttt ttggtgatga tttaccggat caacctgcac aacccttcga aggtttaggc
     6301 tccggtgtca tcatcaacgc cagtaaaggc tatgtgctga ccaacaacca tgtgattaat
     6361 caggcacaga aaatcagtat tcagctcaat gatgggcgcg agtttgatgc aaaactgatt
     6421 ggtagcgatg accagagcga tatcgccctg ttgcaaattc aaaacccgag caaattaacg
     6481 caaatcgcta ttgccgactc cgataaattg cgcgtcggtg attttgccgt ggcggtcggt
     6541 aacccatttg gccttgggca aaccgccacc tctggcattg tttccgcatt aggacgcagc
     6601 gggttgaatc ttgaaggtct ggaaaacttt atccagacag atgcttccat taaccgcggt
     6661 aactccggcg gtgcactgtt aaaccttaac ggtgagttaa ttggcatcaa cactgcaatc
     6721 cttgcgcctg gcggcgggag cgtcggaatt ggatttgcca tccccagtaa tatggcgcga
     6781 acactggcgc agcagcttat cgactttggt gaaatcaaac gcggtttgtt aggcatcaaa
     6841 ggcaccgaga tgagtgccga tatcgccaaa gccttcaacc ttgacgtgca gcgtggcgcg
     6901 tttgtcagcg aagtgttgcc aggttctggc tcggcgaaag cgggcgtcaa aacgggcgat
     6961 attattacca gcctcaacgg caaaccgctg aatagctttg ctgagttgcg ctctcgtatc
     7021 gcgaccaccg agccgggcac gaaagtgaag cttggcctgc tgcgtaacgg caaaccactg
     7081 gaagtagaag tgacgctcga taccagcacc tcttcgtcgg ccagcgctga aatgatcacg
     7141 ccagcgctgg aaggtgcaac gttgagcgat ggtcagctaa aagatggcgg caaaggtatt
     7201 aaaatcgatg aagttgtcaa aggaagccca gctgctcagg ctggcttgca aaaagacgat
     7261 gtgatcattg gcgtcaaccg cgatcgggtg aactcgattg ctgaaatgcg taaagtgctg
     7321 gcggcaaaac cggccatcat cgccctgcaa attgtacgcg gcaatgaaag catctatctg
     7381 ctgatgcgtt aatgtcgtaa accgggcatc aggctgacgt gtgatgtccg gttaactcgt
     7441 ggtatgctgc tgccgttccc ttttttaatg acgcctccat catgtttgtg aagctcttac
     7501 gttccgttgc gattggatta attgtcggcg ctattctgct ggttgccatg ccttcgctgc
     7561 gcagccttaa cccgctttcc actccgcaat ttgacagtac cgatgagacg cctgccagtt
     7621 ataatctggc ggttcgccgc gccgcgccag cggtggttaa cgtttacaac cgtggtttga
     7681 acaccaactc tcacaaccag cttgagatcc gcaccctggg atccggtgta atcatggatc
     7741 aacgcggtta tatcatcacc aataaacacg tcatcaacga cgccgatcag atcatcgtcg
     7801 ccttacagga tggacgtgta tttgaagcat tgctggtggg atctgactct ctaaccgatc
     7861 tggcggtact taaaattaat gccactggcg gtttacctac cattccaatt aatgcacgtc
     7921 gcgtaccgca cattggcgac gtggtactgg cgatcggtaa cccgtacaac ctcgggcaga
     7981 ccattaccca ggggattatt agtgctacgg gtcgaatcgg tctgaacccg accgggcggc
     8041 aaaacttcct acaaaccgat gcttccatta accacggtaa ctctggcggc gcgctggtga
     8101 actcgctggg cgaactgatg ggcattaaca cgctgtcgtt tgataagagt aacgatggcg
     8161 aaacgccgga aggtatcggc tttgcgattc ctttccagtt agcaaccaaa attatggata
     8221 agctgatccg cgatggtcgc gtgatccgcg gctacattgg tatcggcgga cgcgagatcg
     8281 caccactgca cgcgcagggc ggtggtatag atcaactgca agggatcgtg gttaatgaag
     8341 tgtcacctga cggcccggcg gcgaatgcgg gtattcaggt caacgatctg attatttcgg
     8401 tggataacaa accggccatc tctgctctgg agacgatgga tcaggtggca gaaattcgcc
     8461 ctggttcggt gatcccggtt gtagtgatgc gtgatgataa gcagttaacg ctgcaggtca
     8521 ccattcagga atatccggca accaattaag gccagcgtga aagacaaaaa aaccggagtc
     8581 tgtgctccgg ttttttatta tccgctaatc aattacttat taacgaactc ttcgcccagg
     8641 gcgatatctt tcttgagcgt atccagcata ccttccagcg cgttctgttc aaatgcgctc
     8701 agggtaccga tagatttacg ctcttccacg ccgtttttac ccagcagaag cggctgagag
     8761 aagaaacggg cgtactgacc gtcgccttca acgtaggcac attcgacaac gccttgttcg
     8821 ccctgcagtg cgcgaaccag agacaggcca aaacgtgcag cagcctggcc catagacagg
     8881 gttgccgacc cgccaccggc tttcgcttca accacctcag tacccgcgtt ctggatacgt
     8941 ttggtcagat cagccacttc ctgctcggta aaactaacgc caggaacctg tgacaacagc
     9001 ggcagaatgg taacaccaga gtgaccgcca ataaccggca cttcaacttc gcctggctgt
     9061 ttgcctttca gttccgcaac aaaggtgttg gaacgaatga tatccagcgt ggtaacgccg
     9121 aacagtttgt ttttgtcata aacaccggct tttttcagca cttcagcagc aatcgcaact
     9181 gtggtgttaa ccgggttagt gataatacca atgcacgctt tcgggcaggt tttcgcaact
     9241 tgctgtacca ggtttttcac gatgccggcg ttaacattaa acaggtcgga acgatccata
     9301 cccggtttac gcgctacacc tgcagagata agaacgacat ctgcgccttc cagcgccgga
     9361 gtcgcatctt caccagaaaa acctttgatt ttcacagcag tagggatatg gctcagatcg
     9421 acggccacac cgggagtcac tggagcgata tcatacagag agagttctga acctgaaggc
     9481 agttgggttt ttaacagtag tgcaagcgcc tggccaatac cgccagcagc gccgaggact
     9541 gcgactttca tcctaaactc cttattatat tgataaacta agatatgttg ctccgctgcc
     9601 gcgaccttaa tccacaaaat tgccgtttac aatgaccaca tctcaagaat gtgtagtcac
     9661 gcaagtttag cgtttatgca tttaattgcc gtaatcagga acctaagtta cgtaattaac
     9721 aatcgcttat gcattagcgc aacattccaa caggtggtga caatataccc taccgttcag
     9781 ccaaaacaac atcaatttga taacaattaa tttactttta agcagaattt gcatgccgtg
     9841 acgcaggcat atttctcaat aacgaaattt gataaaatcc cgctctttca taacattatt
     9901 tcagccttct tcagggctga ctgtttgcat aaaaattcat ctgtatgcac aataatgttg
     9961 tatcaaccac catatcgggt gacttatgcg aagctcggct aagcaagaag aactagttaa
    10021 agcatttaaa gcattactta aagaagagaa atttagctcc cagggcgaaa tcgtcgccgc
    10081 gttgcaggag caaggctttg acaatattaa tcagtctaaa gtctcgcgga tgttgaccaa
    10141 gtttggtgct gtacgtacac gcaatgccaa aatggaaatg gtttactgcc tgccagctga
    10201 actgggtgta ccaaccacct ccagtccatt gaaaaatctg gtgctggata tcgactacaa
    10261 cgatgcagtt gtcgtgattc ataccagccc tggcgcggcg cagttaattg ctcgcctgct
    10321 ggactcactg ggcaaagcag aaggtattct gggcaccatc gctggcgatg acaccatctt
    10381 taccacccct gctaacggtt tcaccgtcaa agacctgtat gaagcgattt tagagctgtt
    10441 cgaccaggag ctttaatctc tgccccgtcg cttctggcgg cggggaaaat gttgcttatc
    10501 cctctcaacc ccctgctttc ccctgcgatt aatttaacga atagtgcgtt ttactgcgac
    10561 atgtcattca cacaatgaat acataaggta aaaaaagcac attatgcaaa attcattatc
    10621 taattgaaaa aactagaatt aacgataaat aaccgtattt ttaattcttt tttgttatta
    10681 aaattcacat
//
LOCUS       DBFIFI010000152        10401 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.152, whole genome shotgun sequence.
ACCESSION   DBFIFI010000152 DBFIFI010000000
VERSION     DBFIFI010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10401
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.152"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            68..1996
                     /gene="thrS"
                     /locus_tag="N7B39_003577"
     CDS             68..1996
                     /gene="thrS"
                     /locus_tag="N7B39_003577"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HGU2423488.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKIAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2000..2542
                     /gene="infC"
                     /locus_tag="N7B39_003578"
     CDS             2000..2542
                     /gene="infC"
                     /locus_tag="N7B39_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HGU2423489.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            2639..2836
                     /gene="rpmI"
                     /locus_tag="N7B39_003579"
     CDS             2639..2836
                     /gene="rpmI"
                     /locus_tag="N7B39_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HGU2423490.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            2889..3245
                     /gene="rplT"
                     /locus_tag="N7B39_003580"
     CDS             2889..3245
                     /gene="rplT"
                     /locus_tag="N7B39_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HGU2423491.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    3362..3486
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            3368..3412
                     /gene="pheM"
                     /locus_tag="N7B39_003581"
     CDS             3368..3412
                     /gene="pheM"
                     /locus_tag="N7B39_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HGU2423492.1"
                     /translation="MNAAIFRFFFYFST"
     gene            3695..4678
                     /gene="pheS"
                     /locus_tag="N7B39_003582"
     CDS             3695..4678
                     /gene="pheS"
                     /locus_tag="N7B39_003582"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HGU2423493.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            4693..7080
                     /gene="pheT"
                     /locus_tag="N7B39_003583"
     CDS             4693..7080
                     /gene="pheT"
                     /locus_tag="N7B39_003583"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HGU2423494.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHNGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQYKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7085..7384
                     /gene="ihfA"
                     /locus_tag="N7B39_003584"
     CDS             7085..7384
                     /gene="ihfA"
                     /locus_tag="N7B39_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HGU2423495.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            7485..8465
                     /gene="btuC"
                     /locus_tag="N7B39_003585"
     CDS             7485..8465
                     /gene="btuC"
                     /locus_tag="N7B39_003585"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HGU2423496.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     SPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPMLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRALL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            8528..9079
                     /gene="btuE"
                     /locus_tag="N7B39_003586"
     CDS             8528..9079
                     /gene="btuE"
                     /locus_tag="N7B39_003586"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HGU2423497.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLISAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9079..9828
                     /gene="btuD"
                     /locus_tag="N7B39_003587"
     CDS             9079..9828
                     /gene="btuD"
                     /locus_tag="N7B39_003587"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HGU2423498.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     DVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            9906..10370
                     /gene="nlpC"
                     /locus_tag="N7B39_003588"
     CDS             9906..10370
                     /gene="nlpC"
                     /locus_tag="N7B39_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HGU2423499.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
BASE COUNT         2588 a         2482 c         2852 g         2479 t
ORIGIN      
        1 ttaataaaca aatttttctt tgtatgtgat ctttcgtgtg ggtcaccact gcaaataagg
       61 atataaaatg cctgttataa ctcttcctga tggcagtcaa cgccattacg accacgctgt
      121 aagccccatg gatgttgcgc tggacattgg tccaggtctg gcgaaagcct gtatcgcagg
      181 gcgcgttaat ggcgaactgg ttgatgcttg cgatctgatt gaaaacgacg cacaactgtc
      241 gatcattacc gccaaagacg aagaaggtct ggagatcatt cgtcactcct gtgcgcacct
      301 gttagggcac gcgattaaac aactttggcc gcataccaaa atggcaatcg gcccggttat
      361 tgacaacggt ttttattacg acgttgatct tgaccgcacg ttaacccagg aagatgtcga
      421 agcactcgag aagcggatgc atgagcttgc tgagaaaaac tacgacgtca ttaagaagaa
      481 agtcagctgg cacgaagcgc gtgaaacttt cgccaaccgt ggggagagct acaaagtctc
      541 cattcttgac gaaaacatcg cccatgatga caagccaggt ctgtacttcc atgaagaata
      601 tgtcgatatg tgccgcggtc cgcacgtacc gaacatgcgt ttctgccatc atttcaaact
      661 aatgaaaacg gcaggggctt actggcgtgg cgacagcaac aacaaaatgt tgcaacgtat
      721 ttacggtacg gcgtgggcag acaaaaaagc gcttaacgct tacctgcagc gcctggaaga
      781 agccgcgaaa cgcgaccacc gtaaaatcgg taaacagctc gacctgtacc atatgcagga
      841 agaagcgccg ggtatggtat tctggcacaa cgacggctgg accatcttcc gtgaactgga
      901 agtgtttgtt cgttctaaac tgaaagagta ccagtatcag gaagttaaag gtccgttcat
      961 gatggaccgt gtcctgtggg aaaaaaccgg tcactgggac aactacaaag atgcaatgtt
     1021 caccacgtct tctgagaacc gtgaatactg cattaagccg atgaactgcc cgggtcacgt
     1081 acaaattttc aaccaggggc tgaagtctta tcgcgatctg ccgctgcgta tggccgagtt
     1141 tggtagctgc caccgtaacg agccgtcagg ttctctgcat ggcctgatgc gcgtgcgtgg
     1201 attcacccag gatgacgcgc atatcttctg tactgaagaa caaattcgcg atgaagttaa
     1261 cggatgtatc cgtttagtct atgatatgta cagcactttt ggcttcgaga agatcgtcgt
     1321 caaactctcc actcgtcctg aaaagcgtat tggcagcgac gaaatgtggg atcgtgctga
     1381 ggcggacctg gcggttgcgc tggaagaaaa caacatcccg tttgaatatc aactgggtga
     1441 aggcgctttc tacggtccga aaattgaatt taccctgtat gactgcctcg atcgtgcatg
     1501 gcagtgcggt acagtacagc tggacttctc cttgccgtct cgtctgagcg cctcctatgt
     1561 gggcgaagac aacgagcgta aggtaccggt aatgattcac cgcgcaattc tggggtcgat
     1621 ggaacgtttc atcggtatcc tgaccgaaga attcgctggt ttcttcccga cctggcttgc
     1681 gccagttcag gttgttatca tgaatattac cgattcacag tctgaatacg ttaacgaatt
     1741 gacgcaaaaa ctatcaaatg cgggcattcg tgttaaagca gacttgagaa atgagaagat
     1801 tggctttaaa atccgcgagc acactttgcg tcgcgtcccg tatatgctgg tctgtggtga
     1861 taaagaggtg gaatcaggca aaattgccgt tcgcacccgc cgtggtaaag acctgggaag
     1921 catggacgta aatgaagtga tcgagaagct gcaacaagag attcgcagcc gcagtcttaa
     1981 acaattggag gaataaggta ttaaaggcgg aaaacgagtt caaacggcgc gccctaaccg
     2041 tatcaatggc gaaattcgcg cccaggaagt tcgcttaaca ggtctggaag gcgagcagct
     2101 tggtattgtg agtctgagag aagctctgga gaaagcagaa gaagccggag tagacttagt
     2161 cgagatcagc cctaacgccg agccgccggt ttgtcgtata atggattacg gcaaattcct
     2221 ctatgaaaag agcaagtctt ctaaggaaca gaagaaaaag caaaaagtta tccaggttaa
     2281 ggaaattaaa ttccgtcctg gtacagatga aggcgactat caggtaaaac tccgcagcct
     2341 gattcgcttt ctcgaagagg gtgataaagc caaaatcacg ctgcgtttcc gcggtcgtga
     2401 gatggcgcac cagcaaatcg gtatggaagt gcttaatcgc gtgaaagacg atttgcaaga
     2461 actggcagtg gtcgaatcct tcccaacgaa gatcgaaggc cgccagatga tcatggtgct
     2521 cgctcctaag aagaaacagt aaggccttca agtagcactg tctgtggagc cttcgggctt
     2581 cacaggcttt gttcgccttt gtttcgttta ttaacaatgc gaagtggaag ttattaaaat
     2641 gccaaaaatt aagaccgtac gcggtgctgc taagcgcttc aaaaaaaccg gtaaaggtgg
     2701 ttttaagcac aagcacgcta acctgcgtca cattctgacc aaaaaagcga ccaaacgtaa
     2761 acgtcacctg cgtccgaaag ccatggtttc caaaggcgat ctgggcctgg taatcgcgtg
     2821 cctgccgtac gcataagccg ttaacgtttt taacttttta attagaatat agatacagga
     2881 gagcacatat ggctcgcgta aaacgtggtg ttattgcacg cgcacgtcac aagaaaattt
     2941 tgaaacaagc taaaggctac tacggtgcgc gttctcgcgt ataccgcgtt gccttccagg
     3001 ctgttatcaa agctggtcag tatgcttacc gtgaccgtcg tcaacgtaag cgtcagttcc
     3061 gtcaactgtg gattgcgcgt atcaacgcag cagcacgtca gaacggtatt tcttacagca
     3121 aattcatcaa tggcctgaaa aaagcctctg ttgaaatcga ccgtaagatc ctggctgata
     3181 tcgcagtatt cgacaaagta gcgttcaccg ctctggttga aaaagcgaaa gcagctctgg
     3241 cataagccag ttgaaagagg gagctagtct ccctcttttc gtttcaacgc catcaaaaca
     3301 ttgactttta tcgccgtagc cttttcaata aaggtctttt gaagagtacc aaaaggtaac
     3361 gcaagcaatg aatgctgcta ttttccgctt ctttttttac tttagcacct gaatccagga
     3421 ggctagcgcg tgagaagaga aacggaaaac agcgcctgaa agcctcccag tggaggcttt
     3481 ttttgtatgc gcgttttgaa aaataaaaag ccagtctatc aggcgtaatt gtcgcagcca
     3541 gtctggatac ggccagtgcg gagaaaccgg agcgtactta agtacgtgag gatttcgagc
     3601 actgcccggg accaaaatgg caagtaaaat agcctgatgg gataggctct aagtccaacg
     3661 aaccagtgtc accactgaca caatgaggaa aaccatgtca catctcgcag aactggttgc
     3721 cagtgcgaag gcggccatta gccaggcgtc agatgttgcc gcgttagaca atgtgcgcgt
     3781 cgaatatttg ggtaaaaaag ggcacttaac ccttcagatg acgaccctgc gtgagctgcc
     3841 gccagaagag cgtccggcag ccggtgcggt tatcaacgaa gcgaaagagc aggttcagca
     3901 ggcgctgaat gcgcgtaaag cggaactgga aagcgctgca ctgaatgcgc gtctggcggc
     3961 ggaaacgatt gatgtctctc tgccaggtcg tcgcattgaa aacggcggtc tgcatccggt
     4021 tacccgtacc atcgaccgta tcgaaagttt cttcggtgag cttggcttta ccgtggcaac
     4081 cgggccggaa atcgaagacg attatcataa cttcgatgct ctgaacattc ctggtcacca
     4141 cccggcgcgc gctgaccacg acactttctg gtttgacgct acccgcctgc tgcgtaccca
     4201 gacctctggc gtacagatcc gcaccatgaa agcccagcag ccaccgattc gtatcatcgc
     4261 gcctggccgt gtttatcgta acgactacga ccagactcac acgccgatgt tccatcagat
     4321 ggaaggtctg attgttgata ccaacatcag ctttaccaac ctgaaaggca cgctgcacga
     4381 cttcctgcgt aacttctttg aggaagattt gcagattcgc ttccgtcctt cctacttccc
     4441 gtttaccgaa ccttctgcag aagtggatgt catgggtaaa aacggtaaat ggctggaagt
     4501 actgggctgc gggatggtgc atccgaacgt gctgcgtaac gttggcatcg acccggaagt
     4561 ttactctggt ttcgccttcg ggatggggat ggagcgtctg actatgttgc gttacggcgt
     4621 caccgacctg cgttcattct tcgaaaacga tctgcgtttc ctcaaacagt ttaaataagg
     4681 caggaataga ttatgaaatt cagtgaactg tggttacgcg aatgggtgaa cccggcgatt
     4741 gatagcgatg cgctggcgaa tcaaatcact atggcgggcc tggaagttga cggtgtagaa
     4801 ccggttgccg gtagcttcca cggcgtggtc gttggtgaag tggttgagtg tgcgcagcat
     4861 ccgaacgctg acaaactgcg tgtgacaaaa gtgaatgtcg gcggcgatcg cctgctggac
     4921 atcgtctgcg gtgcgccaaa ctgccgtcag ggcctgcgtg tggcggtagc gaccattggt
     4981 gctgttctgc cgggtgattt caaaattaaa gcggcgaaac tgcgcggcga accgtctgaa
     5041 gggatgctgt gctccttttc tgagctggga atttctgacg accataacgg cattatcgaa
     5101 ctgcctgcag atgcgccgat tggcactgac atccgcgaat acctgaaact cgatgacaac
     5161 accatcgaaa tcagcgtaac gccaaaccgt gccgactgtt taggtatcat tggtgttgcg
     5221 cgtgacgttg ccgtgctgaa tcagctgccg ctggttgaac cggaaatcgt tccggttggt
     5281 gcgaccatcg acgacacgct gccgattaca gtcgaagcgc cggaagcctg cccgcgttat
     5341 cttggccgtg tggtaaaagg cattaacgtt aaagcgccaa ctccgctgtg gatgaaagaa
     5401 aaactgcgtc gttgcgggat ccgttctatc gatgcagttg ttgacgtcac caactatgtg
     5461 ctgctcgaat tgggccagcc gatgcacgct ttcgataaag atcgcattga aggcggcatt
     5521 gtggtgcgga tggcgaaaga gggcgaaacg ctggtgctgc tcgacggtac tgaagcgaag
     5581 ctgaatgctg acactctggt catcgccgac cacaacaagg cgctggcgat gggcggcatc
     5641 ttcggtggcg aacactctgg cgtgaatgac gaaacacaaa acgtgctgct ggaatgcgct
     5701 ttctttagcc cgctgtctat caccggtcgt gctcgtcgtc atggcctgca tactgatgcg
     5761 tctcaccgtt atgagcgtgg cgttgatccg gcactgcagt acaaagcgat ggaacgtgcg
     5821 acccgtctgc tgattgacat ctgcggtggt gaggctggtc cggtaattga tatcaccaac
     5881 gaagcaacgc tgccgaagcg tgcaaccatc actttacgtc gtagcaaact ggatcgcctg
     5941 atcggccatc atattgcgga tgagcaggta actgacattc tgcgtcgtct cggctgcgaa
     6001 gtgaccgaag gcaaagacga gtggcaggca gttgcgccga gctggcgttt cgacatggag
     6061 attgaagaag atctggtcga agaagtcgcg cgtgtttacg gctacaacaa catcccggat
     6121 gagccggtac aggcaagcct gattatgggt actcaccgtg aagctgacct gtcgctcaag
     6181 cgcgtgaaaa cgctgctcaa cgataaaggc tatcaggaag tgatcaccta tagcttcgtt
     6241 gatccgaaag tgcagcagat gatccatcca ggcgttgaag ccttactgct gccaagcccg
     6301 atctctgttg aaatgtcagc aatgcgtctt tctctgtgga ccggcctgct ggcaaccgtg
     6361 gtgtacaacc agaaccgtca gcagaaccgt gtgcgcattt tcgaaagcgg tctgcgtttc
     6421 gtaccagata ctcaggcacc gttgggcatt cgtcaggatc tgatgttagc cggtgtgatt
     6481 tgcggtaacc gttacgaaga gcactggaac ctggcaaaag agaccgttga tttctatgat
     6541 ttgaaaggcg atcttgaatc cgttctcgac ctgaccggta aactgaatga ggttgagttc
     6601 cgtgcagaag cgaatccggc actgcatccg gggcaatccg cagcgattta tctgaaaggt
     6661 gaacgtattg gttttgttgg ggttgttcat cctgaactgg aacgtaaact ggatcttaac
     6721 ggtcgcactc tggtgttcga actggagtgg aacaagctcg cagaccgcgt ggtgcctcag
     6781 gcgcgcgaga tttctcgctt cccggcgaac cgtcgtgaca tcgcggtggt ggtcgcagaa
     6841 aacgttcccg cagcggatat tttatccgaa tgtaagaaag ttggcgtaaa tcaggtagtt
     6901 ggcgtaaact tatttgacgt gtaccgcggt aagggtgttg cggaggggta taagagcctc
     6961 gccataagcc tgatcctgca agataccagc cgtacactcg aagaagagga gattgccgct
     7021 accgtcgcca aatgtgtaga ggcattaaaa gagcgattcc aggcatcatt gagggattga
     7081 acctatggcg cttacaaaag ctgaaatgtc agaatatctg tttgataagc ttgggcttag
     7141 caagcgggat gccaaagaac tggttgaact gtttttcgaa gagatccgtc gcgctctgga
     7201 aaacggcgaa caggtgaaac tctctggttt tggtaacttc gatctgcgtg ataagaatca
     7261 acgcccggga cgtaacccga aaacgggcga ggatattccc attacagcac ggcgcgtggt
     7321 gaccttcaga cccgggcaga agttaaaaag ccgggtcgaa aacgcttcgc ccaaagacga
     7381 gtaatctgat ctaactaaaa aggccgctct gcggcctttt ttcttttcac tgtcgaagag
     7441 tcaccgtaaa atcaacgcca tgacacttca gcagaacgga taccatgctg acacttgccc
     7501 gccaacaaca gcgacaaaat attcgctggt tattatgcct gtcagttttg atgctgctgg
     7561 cgcttctctt aagcctttgc gccggtgaac aatggatctc gccaggtgac tggttttctc
     7621 ctcgtggcga actgttcgtc tggcaaattc gcctgccacg tacgctggct gtattgctgg
     7681 ttggtgcggc gctggctata tccggcgctg taatgcaggc gttgtttgaa aatcctctgg
     7741 cagaacctgg actacttggc gtctctaacg gcgcaggcgt ggggcttatc gccgcggtat
     7801 tgcttgggca agggcaactc cccaactggg cgctagggct gtgtgcgatt gctggggcgc
     7861 ttatcatcac tttaatactc ttacgtttcg cccgtcgtca tctttcgacc agtcggttat
     7921 tgctggctgg cgttgcatta gggattatct gtagcgcact aatgacgtgg gctatctact
     7981 tttccacctc tgttgattta cgtcagctga tgtactggat gatgggcggt tttggcggcg
     8041 tagactggcg gcaaagctgg ctgatgctgg cattgatccc catgttgttg tggatctgtt
     8101 gtcagtccag gccgatgaat atgttagcac ttggcgagat ctcggcgcgg caactgggtt
     8161 tacccctgtg gttctggcgc aatgtgctgg tggcagcgac cggctggatg gttggcgtca
     8221 gtgtagcgct ggcgggtgct atcggcttta ttggtctggt gatcccacat attcttcggt
     8281 tgtgtggttt aaccgatcat cgcgcattac ttcccggctg cgcgctggca ggggcgagcg
     8341 cattgctgct ggccgatatt gtagcgcgcc tggcattagc tgccgcagag ctgcctattg
     8401 gcgtggtcac cgcaacgttg ggtgcgccgg tgtttatctg gttattgtta aaagcaggac
     8461 gttagccgca aaaagacggt ctatgattaa aagctaagat ttttactgac cacacccagg
     8521 agaaacgatg caagattcca ttctgacgac cgtagtgaaa gatatcgacg gtgaagtgac
     8581 cacgctggag aagtacgccg gtaatgtgct gttgattgtc aatgtcgcct caaagtgtgg
     8641 cttaacgccg caatatgagc agttggagaa tattcagaaa gcctgggccg atcgaggttt
     8701 tgtggtgctg ggattcccgt gcaaccagtt tctggaacaa gaaccgggca gcgatgaaga
     8761 gattaaaact tactgtacca ccacatgggg ggtgacgttc ccgatgttca gtaagattga
     8821 agttaatggc gaaggacgcc atccgctgta tcaaaaattg atttccgcag cgccgaccgc
     8881 agtcgcgccg gaagagagcg gattctatgc ccgtatggtc agcaaaggtc gtgcaccgct
     8941 gtaccctgat gatattttat ggaattttga gaaattttta gttggcaggg acggattggt
     9001 catccagcgt ttttccccgg atatgacgcc ggaagatccc attgtgatgg aaagcattaa
     9061 actggcgttg gcaaaataat gtctattgtg atgcagttac aggatgttgc ggaatctacc
     9121 cgcctggggc cgctttctgg cgaggttcgg gctggggaga tcctgcacct ggtggggccg
     9181 aatggcgcgg gtaagagtac cttactggcg cgaatggccg gaatgaccag cggtaaggga
     9241 agcattcagt tcgcggggca accactggaa gcatggtccg caacaaaact cgcgctgcat
     9301 cgtgcctatc tttcacaaca gcagacgccg ccgtttgcaa tgccggtctg gcactacctg
     9361 acactgcatc agcacgataa aacgcgtacc gaactactga atgatgtcgc aggggcgctg
     9421 gctcttgatg acaaactcgg tcgtagcacc aatcaacttt ccggcggtga atggcaacgc
     9481 gtacgtcttg ctgcggtggt gctgcaaatc acaccacaag ccaatcccgc aggccaattg
     9541 ctgcttcttg atgagccgat gaacagtctt gatgttgcgc aacaaagtgc gttagacaaa
     9601 attctgagcg cgctgtgtca gcaaggactg gcgattgtga tgagcagtca cgatctcaac
     9661 cacacattgc gtcatgcgca tcgggcgtgg ttgctaaaag gtggaaaaat gctggccagt
     9721 ggacgcagag aagatgtgct cacgccgcca aatctggcgc aggcctatgg gatgaatttt
     9781 cgccgtctgg atatcgaagg tcacagaatg ctgatttcga ccatctgatt tcattagtat
     9841 cttgaaaatc gggtaattac gacgctaaat taatgcaaga ataaaaaaca gaggaaccgt
     9901 acggaatgcg tttctgcctt attttgatca cagcactgtt tctggccggg tgtagccacc
     9961 ataaagcacc gccgccaaat gccagacttt ctgattcgat taccgttatt gccggtttga
    10021 acgaccagct acaaagctgg catggcacgc cgtatcgtta tggtggcatg acgcggcgcg
    10081 gtgtggactg ttcgggattt gtggttgtga cgatgcgcga tcgtttcgat ttgcagctgc
    10141 cccgagaaac caaagaacaa gcctctatcg gcacgcaaat tgataaagac gagttgctgc
    10201 ctggtgacct ggtctttttc aaaacgggtt ccggacaaaa tggtttgcat gtaggtattt
    10261 acgataccaa caaccaattt atccacgcct ctaccagcaa gggagtgatg cgttcctcac
    10321 ttgataatgt ctattggcag aaaaatttct ggcaggcgag acgaatctag tatgtcgaaa
    10381 attggacgtc gatgatacat c
//
LOCUS       DBFIFI010000153        10300 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.153, whole genome shotgun sequence.
ACCESSION   DBFIFI010000153 DBFIFI010000000
VERSION     DBFIFI010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10300
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.153"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            218..1933
                     /gene="yidK"
                     /locus_tag="N7B39_003589"
     CDS             218..1933
                     /gene="yidK"
                     /locus_tag="N7B39_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HGU2423500.1"
                     /translation="MNSLQILSFVGFTLLVAIITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRMIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFLRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            1930..3423
                     /gene="yidJ"
                     /locus_tag="N7B39_003590"
     CDS             1930..3423
                     /gene="yidJ"
                     /locus_tag="N7B39_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HGU2423501.1"
                     /translation="MKRPNFLFIMTDTQAPNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARAEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYELGEKA
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALKPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDAHFADVRSKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDAQPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(3470..3919)
                     /gene="yidI"
                     /locus_tag="N7B39_003591"
     CDS             complement(3470..3919)
                     /gene="yidI"
                     /locus_tag="N7B39_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HGU2423502.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVERKEDVDVDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGARVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            4028..4375
                     /gene="yidH"
                     /locus_tag="N7B39_003592"
     CDS             4028..4375
                     /gene="yidH"
                     /locus_tag="N7B39_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HGU2423503.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            4365..4727
                     /gene="yidG"
                     /locus_tag="N7B39_003593"
     CDS             4365..4727
                     /gene="yidG"
                     /locus_tag="N7B39_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HGU2423504.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            4724..5221
                     /gene="yidF"
                     /locus_tag="N7B39_003594"
     CDS             4724..5221
                     /gene="yidF"
                     /locus_tag="N7B39_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HGU2423505.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PHLEFDDRGDMTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRGCQACDWHLLCPHAREETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(5229..6413)
                     /gene="emrD"
                     /locus_tag="N7B39_003595"
     CDS             complement(5229..6413)
                     /gene="emrD"
                     /locus_tag="N7B39_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HGU2423506.1"
                     /translation="MKRHRNINLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            6495..6569
                     /gene="ysdE"
                     /locus_tag="N7B39_003596"
     CDS             6495..6569
                     /gene="ysdE"
                     /locus_tag="N7B39_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HGU2423507.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            complement(6693..6782)
                     /gene="tisB"
                     /locus_tag="N7B39_003597"
     CDS             complement(6693..6782)
                     /gene="tisB"
                     /locus_tag="N7B39_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HGU2423508.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            7347..7445
                     /gene="ivbL"
                     /locus_tag="N7B39_003598"
     CDS             7347..7445
                     /gene="ivbL"
                     /locus_tag="N7B39_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HGU2423509.1"
                     /translation="MATSMLNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            7551..9239
                     /gene="ilvB"
                     /locus_tag="N7B39_003599"
     CDS             7551..9239
                     /gene="ilvB"
                     /locus_tag="N7B39_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HGU2423510.1"
                     /translation="MASSGTSSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAVAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYKQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            9243..9533
                     /gene="ilvN"
                     /locus_tag="N7B39_003600"
     CDS             9243..9533
                     /gene="ilvN"
                     /locus_tag="N7B39_003600"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HGU2423511.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            9796..>10300
                     /locus_tag="N7B39_003601"
     CDS             9796..>10300
                     /locus_tag="N7B39_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001626294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2423512.1"
                     /translation="MASPINSGIMMANLCPSTFLDKNRNVAAELDIKNNEKKYSPGSN
                     FAKWMLQEIKRLILNIMSGSRSINTDILDYFHPMPGTENNGNRTWVAATGEDEYIEIK
                     QTGDKSFNITLVGRDKPSRKEIPYSGVAVATIIKSLSEKTSALETHSADTVLRKKLVN
                     SIVMKNTD"
BASE COUNT         2549 a         2599 c         2715 g         2437 t
ORIGIN      
        1 attgtatgga gtttcgtttt ttacatctga agtttgcaat tttccattca ttttaatatc
       61 cctccggcga attatgctca caacctgaac ttaacagaaa acaatccgtt cagggatacg
      121 acgtttgtat tttaagaatc tgactgcctg acccgacgca ttttacctct ccctatattc
      181 atgcgtccgg gacataaaca taaataaggg ctatgagatg aattcgttac aaatcttgag
      241 ttttgtcggt tttacgctgc tggtggcgat catcacctgg tggaaggttc gcaaaacaga
      301 taccggatcg caacaaggct attttcttgc cggacgttca ctaaaagcgc cggttattgc
      361 cgcttcgtta atgttaacca acctttccac ggaacaactg gttggtcttt ccgggcaggc
      421 ctacaaaagc ggcatgtcgg tgatgggctg ggaagtgact tcagcggtga cgctgatctt
      481 cctcgcgcta atctttttac cgcgctatct gaagcgcggc attgccacca tccccgattt
      541 tctggaggaa cgttatgata aaacgacgcg tatgatcatc gacttctgct tcctcattgc
      601 caccggcgtc tgctttctgc cgattgttct ctactccggc gcgttggcgc tcaacagcct
      661 gtttcacgtc ggggaatcgc tacagatttc tcacggtgcg gctatctggc tactggtaat
      721 tttgcttggt ctggcgggaa ttttgtatgc ggtgatcggc ggactgcgcg caatggcagt
      781 ggcggactcc atcaacggta ttgggctggt tatcggcggg ttgatggtgc cggtatttgg
      841 cttgatcgcg atgggcaagg gcagctttat gcagggcatt gagcaaatta ccaccgttca
      901 cgccgagaaa ttaaactcag tcggtggccc gaccgatccc ttgccgattg gcgcggcatt
      961 taccggttta attctggtga acacctttta ctggtgtaca aatcagggca tcgtgcaacg
     1021 cacgctggcg tcaaaaagcc tggcggaagg gcaaaagggg gcgctgttaa cggcggtgct
     1081 gaaaatgctc gacccgctgg tactggtgct gccagggttg attgcgtttc atctgtatca
     1141 ggatctaccg aaagccgaca tggcctaccc gacgctggtc aataacgttc tgccagtgcc
     1201 actggtgggt ttcttcggcg cggtgttatt tggtgcagtg atcagtacct tcaacggctt
     1261 tctgaatagc gccagtacgt tattcagtat gggtatttac cgtcgcatca ttaaccagaa
     1321 tgccgagccg cagcagctgg tcaccgtcgg gcgcaaattt ggtttcttta tcgctatcgt
     1381 ttcggtgctg gtagcgccgt ggatcgccaa cgcgccgcag gggctgtata gctggatgaa
     1441 acagctcaac ggcatttaca acgtgccgct ggttaccatc atcattatgg gctttttctt
     1501 cctgcgcatc ccggcgctgg cggcaaaagt ggcgatgggg attggcataa tcagctacat
     1561 caccatcaac tatctggtga agttcgactt ccatttcctc tatgtgctgg cctgtacgtt
     1621 ctgcatcaac gtggtcgtga tgctggtgat cggttttatc aaaccgcgcg ccacgccgtt
     1681 caccttcaaa gatgcgtttg cggtggacat gaaaccgtgg aaaaacgtca agatcgcgtc
     1741 aattggcatc ctgttcgcga tgattggcgt ctatgccggg ctggctgaat tcggcggcta
     1801 cggtacgcgc tggttagcga tgatcagtta tttcattgcc gccgtagtga ttgtctacct
     1861 gatttttgac agctggcggc atcgtcacga cccagccgta acctttactc ccgacgcgaa
     1921 ggatagccta tgaaacgccc caattttctg ttcatcatga ccgataccca ggcccccaat
     1981 atggtcggtt gctatagcgg taaaccgctg aatacgcaaa atattgatag tctggcggcg
     2041 gaaggtattc gctttaattc cgcctacacc tgttcaccgg tttgtacacc ggcgcgcgcc
     2101 gggctgttta ccggtatcta cgctaaccag tccgggccgt ggaccaacaa cgtcgcgccg
     2161 ggtaaaaaca tctccactat ggggcgctac tttaaggatg ccggctatca cacctgttac
     2221 atcggcaaat ggcatctcga cggtcatgac tatttcggca ctggcgagtg tccgccggag
     2281 tgggacgctg attactggtt cgatggggcg aactatctta gcgaactgac ggaaaaagag
     2341 attagcctgt ggcgcaatgg cctaaacagc gtcgaagatt tacaggcgaa ccatattgac
     2401 gaaaccttca cctgggcgca ccgcatcagc aatcgagcgg tggattttct gcaacagccc
     2461 gcgcgcgccg aggaaccctt cctgatggtg gtttcgtatg atgagccgca tcacccgttc
     2521 acctgtccgg tggagtattt agagaaatac gctgattttt actacgagct gggcgagaaa
     2581 gcacaggatg acctggcgaa caaaccagaa catcaccgct tatgggcgca ggcgatgcca
     2641 tcgccagtcg gtgatgacgg gctttatcac catccgctct attttgcctg taatgacttt
     2701 gttgatgacc aaatcggacg ggtcatcaat gccttaaagc cagagcaacg tgaaaatacg
     2761 tgggttattt atacctccga tcacggcgaa atgatgggcg cacataagct gatcagtaaa
     2821 ggggcggcga tgtatgacga catcacccgc attccgctga tcatccgttc gccgcaaggg
     2881 gagcggcgac aggtcgatac gccagtcagt catatcgatt tactgccgac aatgatggcg
     2941 ctggcagata ttgaaaaacc agagattctg ccgggggaaa atatccttgc agtgaaagag
     3001 ccacgcggcg tgatggtgga atttaaccgc tacgagattg agcatgacag ctttggcggt
     3061 tttattccgg tgcgttgctg ggtgacggat gactttaaac tggtgctcaa cctcttcacc
     3121 agtgatgaac tttacgatcg ccgtaatgat ccaaatgaaa tgcataacct gatcgatgat
     3181 gcccattttg ccgacgttcg cagcaaaatg catgatgcct tattggatta tatggacaaa
     3241 attcgcgatc cgttccgcag ttaccaatgg agcctgcgtc cgtggcgtaa agatgcacag
     3301 ccgcgctgga tgggggcatt tcgtccacgc ccacaagatg gttattcgcc agtggtacgc
     3361 gactatgaca ccggcctgcc gacacaaggg gtgaaggtgg aagagaaaaa acagaagttc
     3421 tgacgataat gcccgctgat gaacacccgg cgggcattat tcaggctgct taaaccagtg
     3481 aaccgccagt caggaaggaa aagataagga atatcagcaa aatgctgcac acgatgccga
     3541 taccaaaaag caaggtatgg aaagccagcg tcccgccgcc aaaaacccgc gcgccgcgaa
     3601 tgccccagcg attttcctta cgcataccgc caataaatgc gcaaagtagc gcagcaatgg
     3661 ccagtgcgat accgctttga tcgaggattt tatcgacatc cacgtcctct tttctctcca
     3721 cggtggtaat tttttcgcct tttattccgg cgagaagacc tcttttaatt tcagccgttt
     3781 tgtccgccac gatgctttca agcgttggcg gtggtgcgga aaaagggccg aatgaaaagt
     3841 ggataatccc catcattaaa gcgatggcac cgaatagcat gcccaatgaa ctgattttat
     3901 tttgcgctat gatacccatc tgacctcctt gtagcggatg gcaaaagcat atcaggaaac
     3961 gggcggacga aattcttaaa aaaaaccgct aaaggtagag cgttagtgaa attgcaaagg
     4021 agcaacaatg aaaatttccc gcctcggaga agcgcctgat taccgcttct cgctggctaa
     4081 tgagcgtact tttctggcgt ggatccgtac tgcactagga tttctggcgg cgggggttgg
     4141 gcttgatcaa cttgcgccag atttcgccac gcccgtcatt cgcgaactgc tggcgttgtt
     4201 gttgtgtctg ttttccggcg ggttagcgat gtatggctac ttgcgctggt tacgcaatga
     4261 aaaagcgatg cgtctgaaag aggacctgcc ctacaccaac agcttattaa tcatcagctt
     4321 aatattgatg gtcgtagcag tgatcgttat gggactggtg ttgtatgccg gatagccgca
     4381 aagccagacg cattgccgac ccagggctgc agccggagcg tacatcactg gcgtggtttc
     4441 gtaccatgct gggctacggc gcgttgatgg cgttggctat caaacacaac tggcaccagg
     4501 cgggcatgtt attctggatt tcgattggca tcctcgccat cgtggcgctg atcctctggc
     4561 actacactcg taatcgcaat ttaatggatg tcacgaatag cgatttttcc caatttcacg
     4621 tagtccgtga caaatttttg atctccctcg cggtgttatc tctcgcaata ctgtttgctg
     4681 taacgcatat acatcaactt atcgtattta ttgagagagt cgcatgacag gaagtcaggt
     4741 cattgatgct gaagaggaca ggcataaact ggttgtagag tacaaggatg ctctgcaacc
     4801 tgccgatttt tatcacaatt tcaaacaacg gggcatccgc tccgtacagc ttattcccca
     4861 tcttgaattt gatgaccggg gcgatatgac ggccgcttcg gtgacagcag agctgtgggg
     4921 gaaattttta attgccctgt ttgagtgttg ggtgcgggca gatatcagtc gtatctcgat
     4981 tgagcttttt gacgccacat tacaaaaatg gtgtggcagc gaaaacccgc aaccgcgccg
     5041 tggctgccag gcgtgcgact ggcatctgct gtgtccgcac gctcgggagg agacgccaga
     5101 cagcgtgctt tgtgcgggct atcaggcgtt ttattcttac tctgcgccgc atatgcgggt
     5161 gatgcgtgat ctgataaagc agcatcgttc tcccatggag ctgatgacga tgctgcggtg
     5221 acgtgagctt aaacgggctg cccctgatgc gacatccgcg tcgccagcgg tagccagcac
     5281 agcacgatca acaatcccat taaggtcatc aacaacccca ggctgccctg accggtttgc
     5341 ggcaacatcg cagagagcga cgccagcacg ccggaaccaa tgttttgtag accgccgacc
     5401 agcgcgccag ccgtgcccgc caggaaaggg aacggctcca tcgcgccgct ggtcgccagc
     5461 ggaaacagca tcccggcacc gaaaaagaac agcgcggcgg gaacgagcag cgtccagaca
     5521 ttcatcacgc caaaccagtc ggggatccac atcagtaagc cagccagcag gcagcagata
     5581 accgactgcc acatcaacgt tgagaagcgt ttattaggac gtccggcaaa ccatgcccca
     5641 aaaaatgccg ccggaatcgg cagaataaac aaaatactga ccgtcatact gctcagccct
     5701 aacaccgcgc ccatcagcac gccggagcag gcttcaaagg cggcaatccc ggccagaccg
     5761 ccaatcagca tcagcaaata acagttaaaa ccgctgttac cgaaaagcgt tttataactg
     5821 gtaagcaggc gcgtgcgcgg tgcgtcgacc ggacgcgttt ccggcatcca gcgggccata
     5881 ctgaaggtca caccggcaca aagtaccaac aaaaagagat aacaggcgcg ccagttccac
     5941 atcgtatcca gcagaccgcc gattagcggt gcgagcaacg gactgacaag aattcccatg
     6001 tttaacaggc tgttagcgtg gcgcaactgt gtccgttcat ataaatcacg cggcaaagta
     6061 cgcgccatta cgccgccaac gccggtgccc atcccctgca tcgcgctggc ggcaatcaat
     6121 actgtcaaac tggaggtcgt gaccgcgacc agcgttgcca gcataaaaat ggacattccg
     6181 acgaggatca ccggtcggcg acccacacgg tcggaaatcg ggccataaaa cagctgtgag
     6241 acaccgtaag tcagcagata agcgcccatt acgctctgca ccgccccttc acgaacgttg
     6301 agatcgcgcg ccatatcggc aatagctgga atataaatgg tttgcgccat ctgaccgacg
     6361 gccacgagta ataccaacat caataacaaa ttgatgtttc tatgcctttt cattatcacg
     6421 gatgctttta taaaaataat tagaataagt cactacgcat tcccataaat gcgggagagg
     6481 aatctatcat attgataaat gttagccaga tatacgccag gaatggggaa ttgtttagcg
     6541 gcagaatatg taaacaaaag cggcaataaa tgttgccggg agggatggta ggtcggataa
     6601 gatgcgtcag catcgcatcc gacaccaacc cacacgctaa atataaaatg ggagcggtta
     6661 cccgctcccc tttagtgcga cttgaatctg aattacttca ggtatttcag aacagcatca
     6721 agcagttgca gtgctgcaac aatgagtttg aggataagaa tggcgatatc caccaggctc
     6781 atacgcgtct cctgtggttc aggagcactg ccagctgacg tacctttccg ctgcctgttg
     6841 ccagctgttg tgcacgcggt atctcaagga gaaacgttgt gctcgttgac ataacacagt
     6901 gtgctcacgc ggggttaagg cgctgacggc accacccgtt tcagccagga cttagtgcgc
     6961 cgggtaacga tgcggccccc gcataacaca ttgcgtacag tgatattata gaagcttgct
     7021 gtataaataa acagtaatat ttggacaaaa cgcaaactgt gatcgatact caactatgca
     7081 ctaaatacgt caaaattcgt gccgaaattg cgcgttttgc gcggaacacg tatactttct
     7141 gtgttgacat aatacagtgt gctttgcggt taccagccgc gggcggctga cgaaacctcg
     7201 ctccggcggg gttttttgtt acctgcagta cagtacaaaa cgtgatcaac ccctcaattt
     7261 tcccttgctg aaaaattttc cattgtctcc cctgtaaagc tgtgcttgta taaatattgt
     7321 taaacacaaa accaacaagg tccccaatgg ctacttccat gctcaacgca aaactactac
     7381 caactgcgcc atccgccgca gtggtcgtcg tgcgtgtggt ggtggtcgtc ggcaatgcgc
     7441 cgtagggact ggaacaacac acgattccaa aaccccgccg gcgcaaaccg ggcggggttt
     7501 ttcgtttaag cacctcccgg aaagtcggcc cagaagaaaa ggactggagc atggcaagtt
     7561 cgggcacatc atcgacgcgt aagcgcttta ccggcgcaga atttatcgtt catttcctgg
     7621 aacagcaggg cattaagatt gtgacgggca ttccgggcgg ttctatcctg cctgtttacg
     7681 atgccttaag ccaaagcacg caaatccgcc atattctggc tcgccatgaa cagggcgcgg
     7741 ggtttatcgc tcagggaatg gcgcgcaccg acggtaaacc ggcggtctgt atggcctgta
     7801 gcggaccggg ggcgactaac ctggtgaccg ccattgctga tgcgcggctg gactccatcc
     7861 cgctgatttg catcactggt caggttcccg cctcaatgat cggcactgac gccttccagg
     7921 aagtcgacac ctacggcatc tctatcccca tcaccaaaca caactatctg gtcagacata
     7981 tcgaagaact cccgcaggtc atgagcgatg ccttccgtat tgcgcaatca ggccgccctg
     8041 gcccggtgtg gatagacatt cctaaggatg tgcaaacggc cgtttttgag attgaagcac
     8101 agcccgcggt ggcagaaaaa gccgtcgcgc ccgcctttag cgaagaaagc attcgtgacg
     8161 cagcggcaat gattaatgct gccaaacgcc cggtgcttta tctgggcggt ggtgtgatca
     8221 atgcgcctgc gagagtgcgt gaactggcgg agaaagcgca gctgcctacc acaatgactt
     8281 taatggcgct gggcatgttg ccaaaagcgc atccgttgtc gctgggtatg ttggggatgc
     8341 acggtgtgcg cagcaccaac tatattttgc aggaggcgga tttgttgatt gtgctcggtg
     8401 cgcgttttga tgaccgggcg attggcaaaa ccgagcagtt ctgcccgaat gccaaaatta
     8461 ttcatgtcga tatcgaccgc gcagagctgg gtaaaatcaa gcagccgcac gtagcgattc
     8521 aggcggatgt tgatgacgtg ctggcgcagc tgatcccgca ggtggaagcg caaccgcgtg
     8581 cagagtggca ccagttggta gcggatttgc agcgcgaatt cccgtgtcca atcccgaaag
     8641 cgtgcgatcc gttaagccat tacggcctga tcaacgccgt tgccgcctgt gtcgatgaca
     8701 atgcgattat caccaccgat gtgggtcagc atcagatgtg gacggcgcag gcttatccgc
     8761 tcaatcgccc acgccagtgg ctgacctccg gcgggctggg cacgatgggt tttggcctgc
     8821 ctgcggcgat tggcgcggcg ctggcgaacc cggatcgcaa agtgttgtgt ttctccggcg
     8881 atggcagcct gatgatgaat attcaggaga tggcgaccgc cagcgaaaac cagctggatg
     8941 ttaaaatcat tctgatgaac aacgaagcgc tggggctggt gcatcagcaa cagagtctgt
     9001 tctacaagca gggcgttttt gccgctacct atccgggaaa aatcaacttt atgcagattg
     9061 ccgccggatt cggcctcgaa acctgtgatt tgaataacga agccgatccg caggctgcat
     9121 tgcaggaaat catcaatcgc cctggtccgg cgctgatcca tgtgcgcatt gatgccgaag
     9181 aaaaagtgta cccgatggtg ccgccaggtg cggcgaatac agaaatggtg ggggaataag
     9241 ccatgcaaaa cacaactcat gacaacgtga ttctggaact caccgttcgc aaccatccgg
     9301 gcgtaatgac ccacgtttgt ggcctttttg cccgccgcgc ttttaacgtt gaaggcattc
     9361 tttgtctgcc gattcaggac agcgacaaaa gccatatctg gctactggtc aatgacgacc
     9421 agcgtctgga gcagatgata agccaaatcg ataagctgga agatgtggtg aaagtgcagc
     9481 gtaatcagtc cgatccgacg atgtttaaca agattgcagt gttttttcag taaggagcga
     9541 cattaaaccg ctcgggggag ttactgatga gcgatttttg atagatccgt gcagatatat
     9601 agggtgaaat tgtgccagag accatctcag gcacaattta ttagcccagg acgttaattt
     9661 aagtcgcagc accggtaacc tgggggggac agttagggct aaaattttca gcgatggtgt
     9721 taaattctga ttgctgtcta taccagtggt catgaccagc attctattaa ttaatggttc
     9781 atcgtgaggt atattatggc aagtcctata aacagcggaa taatgatggc aaatctctgc
     9841 ccttccacct ttttggataa aaatagaaat gtcgctgctg agcttgatat taaaaataac
     9901 gagaaaaaat attcacctgg cagtaatttc gcaaaatgga tgttgcagga aataaagcgc
     9961 ttaatactaa acatcatgtc cggttctcgg agtattaaca ccgatattct tgactatttc
    10021 caccctatgc caggcacgga aaataatggt aatcgtacct gggtggcggc aactggagag
    10081 gatgaatata tcgaaatcaa acaaacaggt gataagtctt ttaatattac actggttggc
    10141 agagataaac cttcacgaaa agaaattcca tattcaggag tggctgtagc gactattatt
    10201 aaatcgttgt ctgaaaaaac ttctgcgtta gaaacacatt ccgctgacac ggttttgagg
    10261 aagaaattag ttaatagtat tgttatgaag aatacggatt
//
LOCUS       DBFIFI010000154        10255 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.154, whole genome shotgun sequence.
ACCESSION   DBFIFI010000154 DBFIFI010000000
VERSION     DBFIFI010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10255
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.154"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..1671)
                     /gene="rhsC"
                     /locus_tag="N7B39_003602"
     CDS             complement(<1..1671)
                     /gene="rhsC"
                     /locus_tag="N7B39_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsC"
                     /protein_id="HGU2423513.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAALWQ
                     ALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEVLPAPLPPYRVLTGLVDRFGR
                     TQTFHREAAGEFSGEITGVTDGAGRHFRLVLTTQAQRAEEARQQAISGGTEPSAFPDT
                     LPGYTEYGRDNGIRLSAVWLTHDPEYPENLPAAPLVRYGWTPRGELAVVYDRSGKQVR
                     SFTYDDKYRGRMVAHRHTGRPEIRYRYDSDGRVTEQLNPAGLSYTYQYEKDRITITDS
                     LNRREVLHTQGEGGLKRVVKKEHADGSVTQSQFDAVGRLRAQTDAAGRTTEYSPDVVT
                     GLITRITTPDGRASAFYYNHHSQLTSATGPDGLEIRREYDEWGRLIQETAPDGDITRY
                     RYDNPHSDLPCATEDATGSRKTMTWSRYGQLLSFTDCSGYVTRYDHDRF"
     gene            complement(1831..2043)
                     /gene="rpmE"
                     /locus_tag="N7B39_003603"
     CDS             complement(1831..2043)
                     /gene="rpmE"
                     /locus_tag="N7B39_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HGU2423514.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            2246..4444
                     /gene="priA"
                     /locus_tag="N7B39_003604"
     CDS             2246..4444
                     /gene="priA"
                     /locus_tag="N7B39_003604"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HGU2423515.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMAVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDSEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGHAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            4600..5625
                     /gene="cytR"
                     /locus_tag="N7B39_003605"
     CDS             4600..5625
                     /gene="cytR"
                     /locus_tag="N7B39_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HGU2423516.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            5717..6676
                     /gene="ftsN"
                     /locus_tag="N7B39_003606"
     CDS             5717..6676
                     /gene="ftsN"
                     /locus_tag="N7B39_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HGU2423517.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVTRAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            6769..7299
                     /gene="hslV"
                     /locus_tag="N7B39_003607"
     CDS             6769..7299
                     /gene="hslV"
                     /locus_tag="N7B39_003607"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HGU2423518.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            7309..8640
                     /gene="hslU"
                     /locus_tag="N7B39_003608"
     CDS             7309..8640
                     /gene="hslU"
                     /locus_tag="N7B39_003608"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HGU2423519.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            8707..9633
                     /gene="menA"
                     /locus_tag="N7B39_003609"
     CDS             8707..9633
                     /gene="menA"
                     /locus_tag="N7B39_003609"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HGU2423520.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVVGIFLSQWAA"
     gene            9726..10211
                     /gene="rraA"
                     /locus_tag="N7B39_003610"
     CDS             9726..10211
                     /gene="rraA"
                     /locus_tag="N7B39_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HGU2423521.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
BASE COUNT         2372 a         2765 c         2850 g         2268 t
ORIGIN      
        1 gaagcggtca tggtcataac gggttacata accggaacag tcggtgaagc tcagcaactg
       61 accgtaacgg ctccacgtca tggttttccg gctgccggtg gcatcttccg ttgcgcaggg
      121 taagtcactg tgtggattat cataacggta gcgggtgata tcgccgtcag gggcagtttc
      181 ctgaatcaga cggccccatt catcatattc ccggcgtatt tccagcccgt caggcccggt
      241 ggctgacgtt aactggctgt ggtggttata gtaaaacgcc gatgccctgc catccggcgt
      301 ggtgatgcgc gtgatgaggc ccgtcaccac atccgggctg tattctgttg tcctgcctgc
      361 ggcatccgtc tgtgccctga gcctgcccac cgcgtcaaac tgactctgcg tgacgctgcc
      421 gtccgcgtgt tcctttttca ccacccgctt cagcccgcct tcaccctgcg tgtgcaggac
      481 ttcacggcgg ttcaggctgt cggtgatggt gatgcggtct ttctcatact gatacgtgta
      541 gcttaagcct gccgggttta gctgttctgt cacccgcccg tcgctgtcgt aacggtaacg
      601 gatttccggc cggcccgtgt gacggtgcgc caccatccgg ccccggtatt tatcatcgta
      661 agtaaagctg cgcacctgtt tgccactacg gtcatacacc accgccagtt cgccgcgcgg
      721 cgtccagcca tagcgcacca gcggcgcggc aggtaaattc tccgggtatt ccgggtcgtg
      781 cgtcagccac acggcagaca gacggatgcc gttgtcccgg ccatattcgg tgtaacccgg
      841 cagggtatca ggaaaagcgg acggttccgt cccgccggaa atggcctgct gccgggcttc
      901 ttctgcccgc tgcgcctgcg tggtcagtac cagccggaag tgacgcccgg caccatccgt
      961 cacgccggtg atttcgccgc tgaattcacc ggcggcttcg cggtggaacg tctgtgtgcg
     1021 cccgaagcgg tccaccagcc cggtcagtac ccggtacggc ggcagcggcg caggcagcac
     1081 ctcatccgct tccggcaccc gctcacacca gccgagcagc caccacggcc cctgcggact
     1141 gtttgtcgcc agataacgat gcggacttaa gcggagttct tccggcagcg cctgccagag
     1201 tgcggccagc cggtgacctt catccagttt cgccacgccg ccgcgcacca gccagagtga
     1261 ctcgctgcgg ctgtaaccgt cctcaccggg aaacaggtgc tcaaaataca ggcttctgcc
     1321 gccgttatca ctgagtatca gtgtgttatc gcgcagctgt aagcggatat ccgcaggcat
     1381 tttccagccg gggccgaggc tccccaccgg cgcgggcgtt tttgtccggt aactgctgta
     1441 ggtgcgggag aggatgaacg gcagcgggcc gggcagggcg atgtcggttt caccgggaag
     1501 gacctttgca ccgagcaggg gattgaccgg atggccggac gtcactccgc cggggcacac
     1561 cgaacaggcc acgccggtgg gggcaccaat gcgcaccccg gctgaaccct gaacaatgct
     1621 accgccatac tgcgtcatgt cgccctgacg cgccgccggt tttccgctca tcgtaaaatc
     1681 cttttgaaat aaataagaac cagaagccta gcgccagtca acaacaggaa atgataatga
     1741 gttaacaccc cggaattttt cagaatttat aaaaggcaaa ttacaggcac aaaaaaagcg
     1801 ccgtgcggcg cttttttcgg aaatccggtc ttatttgctg cccgggatgt tgaaacgctt
     1861 gttgaagcgg tcaacacggc caccggtagc aacatcacgc tgtttgccag tgaagaacgg
     1921 gtggcacttg ctgcacacgt cgaggttcag gtcatgacca acggtggagc ggattttcat
     1981 tacgttaccg caagagcagc tagcagtaat ttcttcgtat ttcgggtgaa tatctttttt
     2041 catgggaaaa cctcggttta aggccgcgtc gctcttccag ccctaacgcc agacaccacg
     2101 cgatgttaaa agtatagctt caatacgatc atttcgtacg aaggcgcgaa atcatacaga
     2161 aattaaccag catatgcaaa ctgatccgca ctcttctacg gcaatgtgta tactaaccca
     2221 ccgaatttca agtcaggatg atgctatgcc cgttgcccac gttgccttgc ccgttccgct
     2281 tcctcgtacc tttgactatc tcctgccaga aggcatggcg gttaaagccg ggtgtcgcgt
     2341 gcgcgtgccg tttggcaaac agcaggagcg catcggggtt gtggtgtcag ttagcgacgt
     2401 cagtgagctg ccgcttaacg agctaaaagc ggtagtcgaa gtgctggata gtgagccggt
     2461 gtttactcac tccgtctggc gattgctgct ctgggcggcg gattactatc atcatccgat
     2521 tggcgatgtg ctgtttcatg ccttgccgat tttactgcgc caggggcggc ctgcggcgaa
     2581 cgcgccgatg tggtactggt ttgccactga acaaggccat gcggtggatc tgaacagcct
     2641 gaaacgttcg ccaaagcaac aacaggcact ggcggcattg cggcaaggca aaatctggcg
     2701 cgaccaggtt gccacgcttg aatttaatga tgccgcgtta caggcgctgc gcaaaaaagg
     2761 tctgtgtgat ttagcaagtg aaacaccaga gtttagcgac tggcgaacga actatgccgt
     2821 ttctggtgaa cggttgcgat tgaataccga acaggccacc gccgttggcg caattcatag
     2881 cgcggcggat accttctctg cttggctgct ggcgggcgtt acaggttccg gtaaaacaga
     2941 ggtttatttg agcgtactgg aaaacgtgct cgctcagggc aaacaggcgc tggtgatggt
     3001 gccggaaatc ggcctgacac cgcaaactat cgcccgtttt cgtgaacgtt ttaatgcccc
     3061 cgtagaagtt ctgcattccg gcctgaacga cagcgagcgt ctttcggcgt ggttgaaagc
     3121 gaaaaatggt gaggcggcga ttgtcatcgg cacccgctcc gcgctgttta cgccgtttaa
     3181 aaatctcggt gtgattgtca ttgatgaaga gcacgacagt tcctacaagc agcaggaagg
     3241 ctggcgctat catgcccgcg acctggcggt ttatcgtgcg cacagcgagc aaatcccgat
     3301 tattcttggc tccgcaacac ccgcactgga aacgttatgc aacgtccagc agaaaaaata
     3361 ccgcctgctg cgcctgacgc gtcgggcggg gaatgcgcgt ccggcaattc aacatgtgct
     3421 ggatttaaaa ggtcagaagg tgcaggcggg gctggctccg gcgttaatca cccgtatgcg
     3481 ccagcattta caggccgaca accaggttat cctctttctt aaccgccgtg gctttgcgcc
     3541 tgccttgctg tgccacgact gcggctggat tgccgaatgt ccacgttgcg atcactacta
     3601 cacgctgcat caggcgcaac agcatttgcg ctgtcaccac tgtgacagcc aacgcccggt
     3661 gccgcgccag tgcccttcct gcggttccac gcacctggta cccgtggggc tgggcaccga
     3721 acagcttgaa cagacgctcg cgccgttatt ccctggtgtg cccatttctc gtatcgaccg
     3781 cgataccacc agccgcaaag gagcgctgga acaacaactg gcggaagtgc atcgcggcgg
     3841 cgcgaggata ttgattggca cgcaaatgct ggcgaaaggt caccactttc cggatgtgac
     3901 gctggtagcg ttactggatg tggacggcgc gctgttttct gccgattttc gctcggcaga
     3961 gcgttttgcc cagctataca cccaggtagc aggccgtgcc gggcgtgcgg gtaaacaggg
     4021 cgaagtggtg ctgcaaacgc atcatccgga acacccgcta ttgcaaacgt tgctctataa
     4081 aggctacgac gcttttgccg aacaggcact ggctgagcgg cgaatgatgc aactgccgcc
     4141 gtggaccagc catgtgattg tgcgtgcgga agatcataac aatcagcacg cgccattgtt
     4201 cctgcaacaa ctgcgtaatc tgatcctctc cagcccgctg gcagacgaca aattgtgggt
     4261 tctcggtccg gttccggcac tggcacctaa acgtggcggt cgctggcgct ggcagatatt
     4321 gttgcagcac ccttcccgcg tgcgcttgca acacatcatt aacggtacgc tggcactcat
     4381 caatacaata ccggattccc gtaaggtgaa atgggtgctg gatgttgatc cgattgaggg
     4441 ttaaaccgct cacgatgcga ggcggatcga aaaattcaat attcatcaca cttttcatga
     4501 aaattctgta accgttttca cgcgctatct gctaaaaatg ttgccgatgt gaagtaaaca
     4561 tggatgtagt acgcctgacg tgccaggcga ggagtgagtg tgaaagcgaa gaagcaggaa
     4621 actgccgcga ccatgaaaga cgttgccctc aaggcaaaag tctctacagc gaccgtctcc
     4681 cgagcattaa tgaatcccga taaagtctcc caagccaccc gtaatcgggt tgaaaaagcg
     4741 gcccgtgaag tgggttattt accgcagcct atggggcgca acgtcaagcg taatgaatcc
     4801 cgcaccattc tggtgattgt cccggatatc tgcgatccct tctttagcga aattattcgc
     4861 ggtatcgaag ttacggcggc aaatcacgga tatctggtgc tgattggcga ctgtgcgcat
     4921 caaaatcagc aggaaaaaac ctttatcgat ttgatcatca ccaagcaaat tgatggcatg
     4981 ttgctgctgg gttcaaggct gccgtttgat gccagcattg aggaacagcg taatctgccg
     5041 ccgatggtga tggcgaacga atttgcaccg gaactggagc tgcctaccgt tcatatcgac
     5101 aatctgaccg ccgcatttga tgcagtaaat tatttatatg agcaagggca taaacggatt
     5161 ggctgtatag ccggtcccga agagatgccg ctgtgtcact accgcctgca aggttacgtt
     5221 caggcgctgc gtcgctgcgg cattatggtt gatccgcaat acatcgcccg tggcgacttc
     5281 accttcgaag ccggaagcaa agcgatgcag cagctgcttg atcttccaca accgcctact
     5341 gctgtcttct gccatagcga tgtgatggcg ctcggcgcac tttctcaggc aaaacgccag
     5401 gggctgaaag tcccggaaga cctttccata atcggttttg ataacatcga cctgacgcaa
     5461 ttttgtgatc cgccgctgac aaccatcgcg cagccgcgtt acgaaatcgg tcgggaagct
     5521 atgctgttat tgcttgatca aatgcagggg caacacgttg gcagtggctc tcgtttaatg
     5581 gactgcgaac ttatcatccg gggatcaaca cgcgcgttac cttaaagtaa acctttaaga
     5641 ctgccgtgac tggtcaaagg cccgccgctt aagtaacatg gcgggctgac gaacgaataa
     5701 atacagcgaa acgatagtgg cacaacgaga ttatgtacgc cgcagccaac cggcaccttc
     5761 gcggcgaaaa aagagcacct cacggaaaaa gcaacgaaat ctgcctgcgg tttctcccgc
     5821 tatggtcgct attgctgccg ccgttcttgt gacctttatc ggtggtctgt acttcattac
     5881 gcatcacaag aaagaagagt ccgagacgct gcaaagccag aaagtgaccg gaaacggact
     5941 accaccaaaa ccagaagaac gctggcgcta catcaaagag ctggaaagtc gccagccggg
     6001 tgtgcgtgcg cccacagaac cttctgccgg tggtgaagtg aaaacgccgg agcaactgac
     6061 accagaacaa cgtcagcttc ttgaacaaat gcaggctgat atgcgccagc agccaacgca
     6121 gctggttgaa gttccgtgga atgaacagac gccggaacag cgtcagcaaa cgctacagcg
     6181 ccaacgtcag gcgcagcaat tagcggaaca gcaacgcctg gctcagcagt cgcgtacgac
     6241 tgaacaaagc tggcagcagc agacgcgtac gtcgcaagcc gcgccggtac aggcgcaacc
     6301 gcgccagtct aaaccggctt ctactcagca gccgtaccag gatctgctgc aaacccctgc
     6361 gcacacgacc gcgcagtcga agccgcagca agctgcgccg gttactcgtg ccgctgacgc
     6421 gccaaaaccg acggcggaga aaaaagacga acgccgctgg atggtgcagt gcggttcgtt
     6481 cagaggcgcg gaacaggcag agacggtacg tgctcagctg gcgttcgaag gctttgactc
     6541 aaaaattacc accaacaatg gctggaatcg tgtggtcatt ggcccggtga aaggcaaaga
     6601 gaacgcagac agcaccctca atcggttgaa gatggcgggt catacaaact gcattcggct
     6661 cgccgccggg ggttgaaacc ctcaaaatcc cccccatcta taattgcatt atgccccgta
     6721 cttttgtacg gggtttgtac tctgtattcg taaccaaggg gtcagctcgt gacaactata
     6781 gtaagcgtac gccgtaacgg ccatgtggtc atcgctggtg atggtcaggc cacgttgggc
     6841 aataccgtaa tgaaaggcaa cgtgaaaaag gtccgccgtc tgtacaacga caaagtcatc
     6901 gcgggctttg cgggcggtac tgcggatgct tttacgctgt tcgaactgtt tgaacgtaaa
     6961 ctggaaatgc atcagggcca tctggtcaaa gccgccgttg agctggcaaa agactggcgt
     7021 accgatcgca tgttgcgcaa acttgaagca ctgctggcag tcgcggatga aactgcatcg
     7081 cttatcatca ccggtaacgg tgacgtggtg cagccagaaa acgatcttat tgctatcggc
     7141 tccggcggcc cttacgccca ggctgcggcg cgtgcactgt tagaaaatac tgaacttagt
     7201 gctcgtgaaa ttgctgaaaa ggcgttggat attgcaggcg acatttgcat ctataccaac
     7261 catttccaca ccatcgaaga attaagctac aaagcgtaag gatctcccat gtctgaaatg
     7321 accccacgcg aaatcgtcag cgaactggat aagcacatca tcggccagga caacgccaag
     7381 cgttccgtgg cgattgctct gcgtaaccgc tggcgtcgca tgcagctcaa cgaagagctg
     7441 cgccatgaag tgaccccgaa aaatatcctg atgatcggcc cgaccggtgt cggtaaaact
     7501 gaaatcgccc gtcgtctggc taagctggcg aatgcgccgt tcatcaaagt tgaagcgacc
     7561 aaattcaccg aagtgggcta cgtcggtaag gaagtggatt ctattattcg cgatctgacc
     7621 gatgccgccg tgaaaatggt acgcgtccag gctatcgaga aaaaccgtta tcgcgctgaa
     7681 gaactggcag aagaacgtat tctcgacgtg ctgatcccac ctgctaaaaa caactgggga
     7741 cagaccgaac agcagcagga accgtccgct gctcgtcagg cattccgcaa aaaactgcgt
     7801 gaaggccagc ttgatgacaa agaaatcgag atcgatcttg ccgcagcacc gatgggcgtt
     7861 gaaattatgg ctcctccagg catggaagag atgaccagcc agctgcagtc catgttccag
     7921 aacctgggcg gtcagaagca aaaagcgcgt aagctgaaaa tcaaagacgc catgaagctg
     7981 ctgattgaag aagaagcggc gaaactggtg aacccggaag agctgaagca agacgctatc
     8041 gacgctgttg agcagcacgg gatcgtgttt atcgacgaaa tcgacaaaat ctgtaagcgc
     8101 ggcgagtctt ccggtccgga tgtttctcgt gaaggcgttc agcgagacct gctgccgctg
     8161 gtagaaggtt gcaccgtttc caccaaacac gggatggtca aaactgacca cattctgttt
     8221 atcgcttctg gcgcgttcca gattgcgaaa ccgtctgacc tgatcccgga actgcaaggt
     8281 cgactgccaa tccgcgttga actgcaggca ctgaccacca gcgacttcga gcgtattctg
     8341 accgagccga atgcctctat caccgtgcag tacaaagcac tgatggcgac tgaaggcgta
     8401 aatatcgagt ttaccgactc cggtattaaa cgcatcgcgg aagcggcatg gcaggtgaac
     8461 gaatctaccg aaaacatcgg tgctcgtcgt ttacacactg ttctggagcg tttaatggaa
     8521 gagatttcct acgacgccag cgatttaagc ggtcaaaata tcactattga cgcagattat
     8581 gtgagcaaac atctggatgc gttggtggca gatgaagatc tgagccgttt tatcctataa
     8641 tcgcgttcaa tcattttcat cattgtttga tggggctgaa aggccccatt tttattggcg
     8701 cgtattatga ctgaacaaca aattagccga actcaggcgt ggctggaaag tttacgacct
     8761 aaaaccctcc ccctcgcctt tgctgcaatt atcgtcggga cggcgctggc atggtggcaa
     8821 ggtcattttg atccgctggt cgccctgctg gcactgatta ccgccgggct attacagatc
     8881 ctttctaacc tcgccaatga ttacggcgat gcggtaaaag gcagcgataa acctgaccgt
     8941 atcggaccgc tacgcggcat gcaaaaaggg gtcattaccc agcaagagat gaaacgggcg
     9001 ctcattatta ccgtcgtgct catctgtctc tccgggctgg cactggttgc agtggcgtgc
     9061 catacgctgg ccgattttgt cggtttcctg attcttggcg ggttgtcgat cattgccgct
     9121 atcacctaca ccgtgggcaa tcgtccttat ggttatatcg gtctgggtga tatttccgta
     9181 ctggttttct ttggctggtt aagcgtcatg gggagctggt atttacaggc tcatacattg
     9241 attccggcac tgatccttcc ggcgaccgca tgcggcctgc tggcaacggc agtactgaac
     9301 attaataacc tgcgtgatat caatagcgac cgcgaaaacg gcaaaaacac gctggtggtg
     9361 cgcttaggtg aagtgaacgc gcgtcgttat catgcctgcc tgctgatggg ctctctggta
     9421 tgtctggcgc tgtttaatct cttttcgctg catagcctgt ggggctggct gttcctgctg
     9481 gcggcaccgt tgctggtgaa gcaagcccgt tatgtgatgc gggaaatgga cccggtggcg
     9541 atgcgaccga tgctggaacg cactgtcaag ggagcgttac tgactaacct gctgtttgtt
     9601 gtagggatat tcctaagcca gtgggcagca taactgacaa atatcaatta acaattgatg
     9661 attttgccaa cagaccacat agcgcgatat actgaaaatt ctcgcagcaa ctgaatgtta
     9721 agcctatgaa atacgatact tccgagcttt gtgacatcta tcaagaagat gttaacgtcg
     9781 tggaaccgct gttctccaac tttggcggac gggcgtcgtt tggcggacaa ataatcacgg
     9841 taaaatgttt cgaggacaac gggttgctgt acgatctgct cgaacagaat ggccgtggtc
     9901 gtgttcttgt cgtcgatggc ggtggttctg ttcgtcgcgc actggtcgat gctgaactgg
     9961 cgcgtctggc agtacaaaat gaatgggaag gtctggtcat ttacggcgcg gtgcgtcagg
    10021 tagatgacct ggaagagttg gatatcggca tccaggcgat ggcggcaatt ccggttggtg
    10081 ccgctggcga aggcattggc gaaagcgatg tccgcgtcaa ttttggcggt gttaccttct
    10141 tctccggcga ccatctttat gccgacaata ccgggattat tctttcagaa gatccgctgg
    10201 atattgaata agtatctgcc atgcgttaaa aggggaatta aattcccctt tttta
//
LOCUS       DBFIFI010000155        10198 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.155, whole genome shotgun sequence.
ACCESSION   DBFIFI010000155 DBFIFI010000000
VERSION     DBFIFI010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10198
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.155"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..100
                     /locus_tag="N7B39_003611"
     CDS             <1..100
                     /locus_tag="N7B39_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HGU2423522.1"
                     /translation="AGELPKDRESVFWLNVKNIPPSIDKKIPMFLR"
     gene            253..402
                     /locus_tag="N7B39_003612"
     CDS             253..402
                     /locus_tag="N7B39_003612"
                     /inference="COORDINATES: protein motif:HMM:NF014773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2423523.1"
                     /translation="MDDKDVSLNMIRPFEILTLPIPAGVAGKSLVWRFINDYGAISQP
                     LKKNL"
     gene            424..2958
                     /locus_tag="N7B39_003613"
     CDS             424..2958
                     /locus_tag="N7B39_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HGU2423524.1"
                     /translation="MASSHLFITLASGICLLCSISAFARDSLFNPRLLELDHPADNID
                     IHQFNRSNTLPAGTYKVDVMINGMLFERQEVKFAQDNPDAELHPCYVAIKNVLATYGI
                     KVDAIKSLANVDDKTCVNPVPLIDGATWLLDASKLALNITIPQIYLNNAVNGYISPSR
                     WDQGINAMMMNYDFSASHTIRSNYDDDDDSYYLNLRNGINLGAWRFRNYSTLNSYDGN
                     VDYHSVSNYIQRDIMALRSQIMIGDTWTASDVFDSTQVRGVRLYTDDDMLPSSQNGFA
                     PVVHGIAKTNATVIIKQNGYVIYQSAVPQGAFALTDLNTTSSGGDLDVTIKEEDGSEQ
                     HFIQPFTSLAILKREGQTDVDLSIGEVRDESGFTPEVLQLQAMHGFPLGITLYGGTQL
                     ANDYASAALGIGKDMGALGAISFDVTHARSQFDYDDNESGQSYRFLYSKRFEDTNTTF
                     RLVGYRYSMEGFYTLNEWVSRQDNDSDFWVTGNRRSRFEGTWTQSFTPGWGNIYLTFS
                     RQEYWQTDEVERLLQFGYNNNWRNISWNVSWNYTDSIKRSLGNHHDDNNDDFGKEQIF
                     MFSMSIPLSCWMEDSYVNYSLTQNNHHESTMQVGLNGTMLEGRNLSYNVQESWMHSPD
                     DSYSGNAGMTYDGTYGSVNGSYSWSRDSQHFDYGARGGVLVHSDGVTFSQELGETVAL
                     VKAPGAEGLSIENATGISTDWRGYTVKTQLSPYDENRVALNSDYFSKANIELENTVIN
                     LVPTRGAVVKAEFVTHVGYRVLFNVRQVNGKPIMFGAMATASLETGTVTGIVGDNGEL
                     YLSGMPEKGEFLLSWGQAADEKCKAAYHITHKPDDTSLVQMDAICR"
     gene            2970..4040
                     /locus_tag="N7B39_003614"
     CDS             2970..4040
                     /locus_tag="N7B39_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2423525.1"
                     /translation="MKKILSGLILLLCCPYGFAANGDGATHMSNLSFGPLTVAAANNH
                     SGYNIFEALSNTTGTYPVRCHCDDTHGGPGQQTAFFPIFYTGDAAPGLVLERTLNGLN
                     YYALNDYLSVGVTIFIINNQYAAIPFEHLSNQSTSPQHTCGAGNNGSTVNLDSGRSAK
                     LSFYVRHSITGTVTIPTTEVAWLYAGMSDHFPKTTPVSKVTIRGQLTAPQNCELTPNQ
                     SIDVDFQKINSAEFSSTAGSIIAERKIKTEVTVSCTGMEDVRSTEVVSASMIAANRSA
                     DATMIVTSNPDVGIKIFDKNDRPVNVDGGNLPADMGAISRLGKTDGSVTFYSAPASLT
                     GAKPAPDNGFTATATLVIEFTN"
     gene            4068..5150
                     /locus_tag="N7B39_003615"
     CDS             4068..5150
                     /locus_tag="N7B39_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2423526.1"
                     /translation="MKKWHYIFCIILFHLGLPCGYAANDGTCATRGGTHTLSLNFPLT
                     TVSAANNVPGNTLIDIANATSSENYSVLCNCDSKHSNGAYHEIYYTADPAPGMVYSTT
                     ASGLAFYYLNEYVDVGTKISVLNAGYTAVPFEHVSNQATTTDHTCQGNKTTAVGVSLK
                     TGADAKISFRIKRSINGTVVIPITDIALLYANISSTTTRGEAIAKVRISGSLTAPQSC
                     QINAGQVIYFDFDTIPASEFSSTAGQAITSRKITKTVSIECTGMGYERTQKVDASFTG
                     TNRSSDDTMVATDNADVGIKIYNKSNAEVSVNNGKLPADMGNTTIFGRKNGSVTFSAA
                     PASFTGARPQPGVFNATATLTIEFVN"
     gene            5362..6402
                     /gene="pstS"
                     /locus_tag="N7B39_003616"
     CDS             5362..6402
                     /gene="pstS"
                     /locus_tag="N7B39_003616"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HGU2423527.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVLPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            6489..7448
                     /gene="pstC"
                     /locus_tag="N7B39_003617"
     CDS             6489..7448
                     /gene="pstC"
                     /locus_tag="N7B39_003617"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HGU2423528.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            7448..8338
                     /gene="pstA"
                     /locus_tag="N7B39_003618"
     CDS             7448..8338
                     /gene="pstA"
                     /locus_tag="N7B39_003618"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HGU2423529.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            8521..9294
                     /gene="pstB"
                     /locus_tag="N7B39_003619"
     CDS             8521..9294
                     /gene="pstB"
                     /locus_tag="N7B39_003619"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HGU2423530.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            9309..10034
                     /gene="phoU"
                     /locus_tag="N7B39_003620"
     CDS             9309..10034
                     /gene="phoU"
                     /locus_tag="N7B39_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HGU2423531.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDPKE"
BASE COUNT         2590 a         2438 c         2599 g         2571 t
ORIGIN      
        1 ggctggcgag ttaccaaaag atcgggagtc cgtattctgg ttgaacgtga aaaacatccc
       61 tccttcaatt gataaaaaaa taccaatgtt cttgagatag ccataaaaac aagaattaag
      121 ttattctggc gaccagccgc ggtaaagata atccctgagc aggctattaa aaatgttaaa
      181 tggtcgcggc agggtaataa tctcatcgtc gaaaatccaa atccgatcca tattaatgta
      241 atgagtgtga cggtggatga taaagacgtg tcgctaaata tgatccgccc ttttgaaata
      301 ttgacattac caattccggc tggcgtggca ggaaaatcgc tcgtttggcg ctttattaat
      361 gattacggtg caattagtca accactaaag aaaaaccttt aagcgccgga taaggatata
      421 tcgatggcct cttcgcatct tttcattacg cttgcatcgg gcatatgtct gctctgttcc
      481 atatctgctt ttgcccggga tagcttgttc aaccccagat tactggaact ggatcatcct
      541 gcggataata ttgatattca ccagttcaac cgttcgaata ccttacctgc gggaacatac
      601 aaagttgatg tgatgatcaa cggcatgctc ttcgaacgcc aggaagttaa attcgcccag
      661 gataaccctg atgctgaact ccacccatgc tacgtggcga taaaaaacgt gctggcgacc
      721 tatggtataa aagttgatgc gataaaatct ctggcgaatg ttgatgacaa aacatgcgta
      781 aatccagttc cgctgatcga cggggctacc tggttactgg acgccagtaa acttgcattg
      841 aatattacta ttccgcaaat ttatctcaac aatgcagtta atggttatat cagcccttcc
      901 cgttgggatc aggggatcaa tgccatgatg atgaattatg atttttcggc atcgcatacc
      961 atccggtcaa attatgacga cgacgatgac agttattatc tgaatttgcg taatggtatt
     1021 aatttaggcg catggcgttt tcgtaattac agcaccctga attcttatga cggtaatgtg
     1081 gactaccatt ccgtcagtaa ttacattcag cgcgacatca tggcattacg tagccagatt
     1141 atgattggcg atacctggac ggcaagcgat gtatttgata gtacacaggt gcgtggcgtg
     1201 cggctgtata ccgatgacga tatgttgccc tccagccaga acggctttgc gccagtggta
     1261 catgggattg cgaaaactaa cgccacggtg atcatcaaac aaaacggcta cgttatttat
     1321 caatcagccg taccacaggg cgcatttgcc ctcaccgact taaacacgac cagtagcggc
     1381 ggcgatctcg atgtcactat caaagaagaa gatggcagcg agcagcactt tattcagcca
     1441 tttacttcac tggccattct caagcgtgaa ggtcagaccg atgtagacct tagcattgga
     1501 gaagtgcgcg acgaaagcgg ctttacgcct gaggtcttgc agttacaagc aatgcacggt
     1561 ttccctttgg gaataacttt gtatggcgga acacaattgg caaatgatta cgcttctgcc
     1621 gcgctgggta ttggtaaaga tatgggggcg ctgggcgcga tttcttttga cgtgactcat
     1681 gcccgctcgc agtttgacta cgacgataat gagagtggtc aatcgtatcg ttttctctat
     1741 tccaaacgtt ttgaagacac caataccacc tttcgtctgg tgggttatcg ctactctatg
     1801 gaggggttct acaccctcaa tgaatgggtg tcgcgacagg ataatgattc tgatttctgg
     1861 gtaacgggca accgtcgcag ccgcttcgaa ggtacctgga cgcaatcttt cacgccaggc
     1921 tggggcaata tttatttaac attcagtcga caggaatact ggcagaccga tgaggtcgaa
     1981 cgtttattac agttcggcta taacaacaac tggcgaaaca tctcctggaa cgtttcctgg
     2041 aactatacgg actcgatcaa gcgctcattg ggcaaccatc atgatgataa caatgatgat
     2101 ttcggcaaag aacagatttt catgttctca atgtcgatac cgctatcgtg ctggatggaa
     2161 gacagctacg tcaactattc gttaacgcaa aacaaccacc atgaaagcac gatgcaggtc
     2221 ggtctgaacg gaacgatgct ggaagggcgt aacctgtctt ataacgtaca ggaatcgtgg
     2281 atgcactctc ctgatgactc ctacagcggc aatgccggaa tgacctatga cgggacttat
     2341 ggctcggtca atggtagcta ttcctggagc cgtgactccc aacattttga ttatggcgcc
     2401 agaggcggcg tgctggtgca tagtgacgga gtgaccttct cgcaggaact gggcgaaacg
     2461 gtggcattgg tcaaagcgcc gggcgcagaa ggcctgtcca ttgaaaacgc caccgggatt
     2521 tctaccgact ggcgtggtta taccgtaaaa acgcagctta gcccgtatga cgaaaaccgc
     2581 gtggcattga acagcgacta tttctccaaa gccaatattg aactggaaaa caccgtcatc
     2641 aacctggtac caacgcgcgg tgcggtggtg aaagccgaat ttgtcaccca tgtcggttat
     2701 cgcgtgctat ttaacgtccg ccaggtcaac ggtaaaccaa taatgtttgg cgcgatggca
     2761 accgcctctc tcgaaacggg cacagtcacc gggattgtcg gtgataacgg cgaactgtat
     2821 ctctccggga tgcctgaaaa aggcgagttt ttattgagtt ggggacaagc tgcggatgaa
     2881 aaatgtaagg cggcctatca catcacccat aaacctgatg ataccagcct ggttcaaatg
     2941 gatgcgattt gtcgctaaag gaagcatgaa tgaaaaaaat attatcaggg ttgattctgc
     3001 tgctttgctg tccttatggt ttcgccgcta atggtgatgg cgcaacgcac atgtcaaatt
     3061 tatcatttgg tccgctgacg gtggcagcgg cgaataacca ctccggatac aatattttcg
     3121 aggcactgag caacacgact ggaacatacc cggtgcgctg tcactgtgat gacacgcatg
     3181 gcggaccggg ccaacaaaca gcattttttc ctatcttcta cacgggagat gccgcaccgg
     3241 ggcttgtgct tgagcgcact cttaatgggt taaattacta tgctctgaat gattatttat
     3301 cggtcggcgt gacgattttt attattaata accaatatgc ggccattcct tttgaacact
     3361 tatccaacca atccacctca ccgcaacata cctgtggagc aggtaataat gggagcactg
     3421 taaatctgga ttcagggcgc tcggcaaaat tatctttcta tgttcggcat tctattactg
     3481 gcacggtgac aatacccaca acggaagtcg cctggttgta cgcgggcatg tccgatcatt
     3541 ttcccaaaac gacccccgtt tctaaagtga caattcgcgg acaactaacg gccccgcaga
     3601 actgtgagtt aacgccaaat cagagcatcg atgtcgattt tcaaaaaatt aatagcgctg
     3661 agttctcctc aacggcgggt tcaattattg cagaaagaaa gattaaaacc gaagtcacag
     3721 tatcctgtac cgggatggaa gacgtaaggt ccacggaggt ggtgagtgcg tcgatgattg
     3781 cggcaaacag aagtgccgat gccaccatga tcgtgacgag taatccggat gtgggaatta
     3841 agatttttga taagaacgac cgtccagtga atgtggatgg gggcaactta cccgctgata
     3901 tgggtgctat tagtcgatta ggaaaaaccg atggtagcgt aacgttttat tcagcgcccg
     3961 ccagtctgac gggcgcaaaa ccagcgcctg ataatggatt taccgctaca gccacgctgg
     4021 ttattgaatt tactaactaa tcttgtgtta gtaaaaggaa taatttcatg aaaaaatggc
     4081 attatatatt ttgcataatt ctctttcatt tagggttacc gtgcgggtat gcggcaaatg
     4141 atggcacgtg tgcaacaaga ggcggcacac atacattaag ccttaatttt cctctgacaa
     4201 cggtcagtgc agcaaacaat gtgcctggaa atacattaat agatattgct aatgcaacat
     4261 cttctgaaaa ttatagcgtt ctgtgtaact gtgattcaaa acatagcaat ggcgcttatc
     4321 acgaaatata ttataccgca gaccctgctc ccggtatggt ttatagcacc accgcaagtg
     4381 gtcttgcttt ttactatctt aacgaatatg tcgatgtggg aacaaaaata tctgtgctaa
     4441 atgcggggta tacggcagtt ccttttgaac atgtttccaa ccaggcaact acaacagatc
     4501 acacttgtca gggaaacaaa actacagcgg ttggcgtgag cctgaaaact ggagcagatg
     4561 cgaagatttc atttcgtatt aaacgttcaa taaatggaac ggtagtaata cctatcaccg
     4621 atattgcatt gctgtatgcc aacatatcca gcaccacgac ccgtggtgag gcgattgcaa
     4681 aagttcgaat ttcaggcagt ttgaccgcac cacagtcttg tcagataaat gcaggacagg
     4741 tgatttattt tgattttgat actattcctg cgtccgaatt ttcatctacc gccgggcaag
     4801 ccattacttc acgaaaaatc actaaaacag tgagtattga gtgtacgggg atggggtatg
     4861 agcgtacgca gaaagtcgat gcttctttta cggggacgaa ccgaagcagt gacgatacga
     4921 tggtggcgac agacaatgct gatgtcggga tcaaaattta caataaatcg aatgctgaag
     4981 ttagcgtcaa caacggcaag ttacccgcag acatgggcaa cacgaccatt tttggtcgta
     5041 aaaatggttc ggtaactttt tcggcagcac ctgccagctt taccggtgcc cggcctcagc
     5101 ccggcgtttt taacgctacc gcgaccttaa ccattgaatt tgtaaactaa ccttttgcgg
     5161 ctgttttatg acagccgttc atatttcctc tcctttcatg aaaaaatgct gatatttttc
     5221 cccttcaata tcaataagta ataactttcc tttgggtgtc atcaaactgt cacattcctt
     5281 acatataact gtcacctgtt tgtcctattt tgcttctcgt agccaacaaa caatgcttta
     5341 tgaatcctcc caggagacat tatgaaagtt atgcgtacca ccgtcgcaac tgttgtcgcc
     5401 gcgaccttat cgatgagtgc tttctctgtg tttgcagaag caagcctgac aggtgcaggt
     5461 gcaaccttcc ctgcgccggt gtatgccaaa tgggctgaca cttaccagaa agaaaccggt
     5521 aataaagtta attaccaggg tatcggttct tccggtggcg taaaacagat tatcgccaat
     5581 accgttgatt ttggtgcttc tgacgcgccg ctgtctgacg aaaaactggc tcaggaaggt
     5641 ctgttccagt tcccgaccgt gattggcggc gtggtgctgg cggttaacat tccaggtctg
     5701 aagtctggtg agctggtgct ggatggtaaa accctcggcg atatctacct gggcaaaatc
     5761 aagaagtggg atgatgaagc catcgccaaa ttgaatccgg gtctgaaact gccttcacag
     5821 aacattgctg tagtgcgccg cgcagatggc tccgggactt ccttcgtctt caccagctac
     5881 ctggcgaaag tgaatgaaga gtggaaaaac aacgttggta ctggctctac cgtaaaatgg
     5941 ccgatcggtc tgggtggtaa aggtaacgac ggtatcgctg cgttcgttca gcgtctgccg
     6001 ggtgcaattg gttacgttga atatgcttat gcgaagcaaa acaacctggc gtacaccaaa
     6061 ctgatctccg ctgatggtaa acctgttttg ccgaccgaag aaaacttcgc taatgcagca
     6121 aaaggtgcag actggagcaa aaccttcgct caggatctga ccaaccagaa aggcgaagat
     6181 gcgtggccta ttacctctac cacgttcatt ctgatccaca aagatcagaa gaaaccagaa
     6241 caaggcacag aagtgctgaa attcttcgac tgggcgtaca aaaccggggc taaacaggcg
     6301 aacgacctgg attacgccag cctgccggat agcgtagttg aacaggttcg cgctgcgtgg
     6361 aagaccaata ttaaagacag tagcggcaag ccgctgtact aataaaactc caggccggat
     6421 aaggcattaa acggcttatc cggcattaca aaatgacttt gtaaacgcgt ttaactgaag
     6481 agtaacttat ggctgcaacc aagcctgctt ttaacccacc gggtaaaaag ggcgacataa
     6541 ttttcagcgt gctggtaaaa ctggcggcgc tgattgtgct attgatgttg ggtggcatta
     6601 ttgtctctct gatcatctcc tcatggccga gcattcagaa atttggtctg gctttcctgt
     6661 ggaccaaaga gtgggatgcg ccgaacgata tctacggggc gctggtgccg atctacggta
     6721 cgttggtgac ttcgtttatc gcgctgctga tcgccgtccc ggtgagtttc ggtatcgccc
     6781 tgttcctgac tgagcttgcg cctggctggc tgaaacgccc gctgggtatc gccattgagc
     6841 tgctggcagc cattccaagt atcgtttacg gcatgtgggg cctgtttatc tttgcgccgc
     6901 tgttcgccgt ttacttccag gagccggtcg gcaatatcat gtcgaatatc ccgattgtcg
     6961 gcgcgctgtt ctctggcccg gcatttggta tcggtatcct cgcggcaggc gtgatcctcg
     7021 ccatcatgat tattccgtac attgcggcgg taatgcgcga tgtgttcgaa caaaccccgg
     7081 tgatgatgaa agagtcggcc tacggtattg gctgcaccac ctgggaggtt atctggcgta
     7141 tcgttcttcc gttcaccaaa aatggtgtta tcggcggcat tatgctgggg ctgggccgcg
     7201 cgcttggtga aaccatggcg gtgaccttta ttatcggtaa cacctaccag ctcgacagcg
     7261 cctcgctgta catgccgggc aacagtatca cctctgcgct ggcgaacgaa tttgccgaag
     7321 cggaatccgg tctgcacgtt gccgcactga tggaactggg cctgatcctg tttgtgatta
     7381 ccttcatcgt cctcgccgca tcgaagttta tgattatgcg cctggctaag aatgaggggg
     7441 ctcgctaatg gctatggttg aaatgcaaac cactgcggcg ctggcggaat cccgccgcaa
     7501 aatgcaggcg cgtcgccgcc tcaaaaaccg tattgcactg acgctctcaa tggcgacgat
     7561 ggccttcggt ctgttctggc tgatctggat tttaatgtcc accatcaccc gcggtatcga
     7621 cggtatgtcg ctggcgctgt tcactgaaat gacgccgccg cccaatacgg aaggtggtgg
     7681 tctggcgaac gctctggcag gtagcgggct gttaattttg tgggccacgg tattcggtac
     7741 gccgctgggc attatggcgg ggatttatct ggctgaatat ggtcgtaaat cctggctggc
     7801 agaagtgatt cgcttcatta acgacattct gctctctgcg ccgtcgattg tggttggtct
     7861 gtttgtttac accatcgtgg tggcgcagat ggagcacttc tccggttggg cgggtgtgat
     7921 tgcgctggcg ttgttgcagg tgccgattgt tatccgcacc accgagaaca tgctgaaact
     7981 ggtgccggac agcctgcgtg aagcggctta tgcgctgggt acaccgaagt ggaagatgat
     8041 ctccgcgatt acgctgaaag cgtcggtgtc cgggatcatg accggtatcc tgctggcgat
     8101 tgcccgtatt gctggtgaaa ccgcgccgct gctgtttacc gcgctctcca accagttctg
     8161 gagcacggac atgatgcagc cgatcgccaa cctgccggtg acgatcttta agtttgcgat
     8221 gagcccgttt gcggaatggc agcaactggc ctgggccggg gtattgatca ttaccctgtg
     8281 cgtactgctg ctgaacattc tggcgcgcgt tgttttcgcg aagaataaac acggttgata
     8341 ttgctgacac ggttttcccc tcaccctaac cctctcccca gaggggcgag gggaccgacc
     8401 gagcgccttt ttgactctgt acacggttaa cactttgccg gatgcggcgt gaacgcctga
     8461 tccggcctac ggtaagcctg attcgcgaag tgcatcaggc acgatgagga aaagattgca
     8521 atgagtatgg ttgaaactgc cccgagtaaa attcaggttc gtaatttgaa cttctactac
     8581 ggcaaattcc atgccctgaa aaacatcaac ctggatatcg ccaaaaacca ggtaacggcg
     8641 tttatcgggc cgtccggctg cggtaaatcg acgctgctgc gtaccttcaa caaaatgttt
     8701 gaactgtacc cggaacagcg tgcggaaggt gaaattctgc tcgatggcga caacatcctg
     8761 accaactctc aggatatcgc cctgctgcgt gcgaaagtgg gcatggtgtt ccagaaaccg
     8821 acgccgttcc cgatgtccat ctacgacaac atcgcctttg gcgttcgtct gtttgagaag
     8881 ctctctcgtg ccgacatgga cgagcgcgtg cagtgggcat tgaccaaagc cgcattgtgg
     8941 aacgaaacca aagataaatt gcatcagagc ggttactctc tctctggtgg tcagcaacag
     9001 cgtctgtgca ttgcgcgtgg tatcgccatt cgcccggaag tgctgctgct cgacgaacca
     9061 tgttcggcgc tcgaccccat ctccaccggg cgtattgaag agctgatcac cgaactgaag
     9121 caggattaca ccgtggtgat cgtcacccac aacatgcagc aggctgcgcg ttgttccgac
     9181 cacacggcgt ttatgtacct gggcgaattg attgagttca gcaacacgga cgatctgttc
     9241 accaagccag cgaagaaaca aacagaagac tacatcaccg gtcgttacgg ttgattcagg
     9301 agtgcgttat ggacagtctc aatcttaata aacatatttc cggccagttc aacgccgaac
     9361 tggaaagtat ccgcacgcag gtgatgacca tgggcggcat ggtggagcag cagctttctg
     9421 atgcaatcac cgcgatgcat aaccaggaca gcgatctggc gaagcgcgtg atcgaaggcg
     9481 acaagaacgt caacatgatg gaagtggcga tcgatgaagc ctgcgtgcgc attattgcca
     9541 aacgccagcc gacggcgagc gacctgcgat tggttatggt gatcagtaaa accattgccg
     9601 aactggagcg tattggcgac gtggcggaca aaatctgccg tactgcgctg gagaaattct
     9661 cccagcagca tcagccgttg ctggtaagtt tggagtcgct gggccgtcat actatccaga
     9721 tgctgcacga cgtgctggac gcgttcgcgc ggatggacat tgacgaagcg gtacgtattt
     9781 atcgtgaaga taaaaaagtc gatcaggagt acgaaggtat tgttcgtcaa ctgatgacct
     9841 acatgatgga agattcgcgc accattccga gtgtacttac cgcgctgttc tgtgcgcgtt
     9901 ctatcgaacg tattggcgac cgctgccaga atatttgtga gtttatcttc tactacgtga
     9961 agggtcagga tttccgtcat gtaggcggtg atgagctgga taaactgctg gcgggaaaag
    10021 accccaaaga gtagtgctaa ctgtaggcca gataaagctt tttgccgcat ctggcaatca
    10081 gcccggtcat acatagttac cgggctgata cactttctca ctgaaatatt gccacacaaa
    10141 ctcacctcga aactctcccc agtacattga atatacatta tttcatttga attatttt
//
LOCUS       DBFIFI010000156        10103 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.156, whole genome shotgun sequence.
ACCESSION   DBFIFI010000156 DBFIFI010000000
VERSION     DBFIFI010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10103
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.156"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            195..467
                     /gene="ynjH"
                     /locus_tag="N7B39_003621"
     CDS             195..467
                     /gene="ynjH"
                     /locus_tag="N7B39_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HGU2423532.1"
                     /translation="MSRALFAVVLAFPLIALANPHYGPDVEVNVPPEVVSSGGQSAQP
                     CTQCCVYQDKNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            complement(433..840)
                     /gene="nudG"
                     /locus_tag="N7B39_003622"
     CDS             complement(433..840)
                     /gene="nudG"
                     /locus_tag="N7B39_003622"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HGU2423533.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVEPDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            927..1547
                     /gene="ynjF"
                     /locus_tag="N7B39_003623"
     CDS             927..1547
                     /gene="ynjF"
                     /locus_tag="N7B39_003623"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HGU2423534.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPQQNALAGGWLLFAFIGTGSSFLAFAALAAKYQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(1556..2863)
                     /gene="ynjE"
                     /locus_tag="N7B39_003624"
     CDS             complement(1556..2863)
                     /gene="ynjE"
                     /locus_tag="N7B39_003624"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HGU2423535.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRSSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNGKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAARGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAAARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPDQQVSFYCGTGWRAS
                     ETFMYARAMGWNNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(2930..3583)
                     /gene="ynjD"
                     /locus_tag="N7B39_003625"
     CDS             complement(2930..3583)
                     /gene="ynjD"
                     /locus_tag="N7B39_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGU2423536.1"
                     /translation="MLCVKNVSLRLPESCLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNTRRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(3583..5118)
                     /gene="ynjC"
                     /locus_tag="N7B39_003626"
     CDS             complement(3583..5118)
                     /gene="ynjC"
                     /locus_tag="N7B39_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HGU2423537.1"
                     /translation="MATPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCTRLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDKLGIGLGFTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLGYLLWCTWRRTIPRVDGVRKP
                     ATPLLPGITLASFLPLTSVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNMDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSRAKIFFYVKCPLMLRPTLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAALALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            complement(5091..6257)
                     /gene="ynjB"
                     /locus_tag="N7B39_003627"
     CDS             complement(5091..6257)
                     /gene="ynjB"
                     /locus_tag="N7B39_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGU2423538.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTDWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALNEAPNDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTFRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPAV
                     WGDPSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            complement(6267..6815)
                     /gene="ynjA"
                     /locus_tag="N7B39_003628"
     CDS             complement(6267..6815)
                     /gene="ynjA"
                     /locus_tag="N7B39_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HGU2423539.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSSDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQELTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            complement(6815..7522)
                     /gene="ydjZ"
                     /locus_tag="N7B39_003629"
     CDS             complement(6815..7522)
                     /gene="ydjZ"
                     /locus_tag="N7B39_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HGU2423540.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGSLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            complement(7537..8214)
                     /gene="ydjY"
                     /locus_tag="N7B39_003630"
     CDS             complement(7537..8214)
                     /gene="ydjY"
                     /locus_tag="N7B39_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HGU2423541.1"
                     /translation="MLQHYSVSWKKGLTALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMGYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKTYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPANNTLAT
                     VTFKITE"
     gene            complement(8219..8929)
                     /gene="ydjX"
                     /locus_tag="N7B39_003631"
     CDS             complement(8219..8929)
                     /gene="ydjX"
                     /locus_tag="N7B39_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HGU2423542.1"
                     /translation="MNAERKFLFACLIFALAIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPFLGTLLSLIAATLASSCSFLLARW
                     MGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRNPLTHPKNEG"
     gene            complement(9096..9902)
                     /gene="xthA"
                     /locus_tag="N7B39_003632"
     CDS             complement(9096..9902)
                     /gene="xthA"
                     /locus_tag="N7B39_003632"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HGU2423543.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDEEEAQRRIIMAEIPSPLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRENPVLIMGDMNISPGDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMERLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
BASE COUNT         2478 a         2669 c         2619 g         2337 t
ORIGIN      
        1 tgtttatgtc atgtgctttt gcagttttcg actataccat caagaatgtg acggtaagca
       61 agaaaaaaat gacccaggaa agcaaattaa taacgagagt aatctcataa ccaaatgcga
      121 taactcagac gctaaagttt tgccacccgg ctaccgttat cagtagcaga gacaataacg
      181 ttaaggagtt ccctgtgagt cgagcattgt tcgccgtagt attagcattt ccattaattg
      241 cactggctaa cccgcattat ggtccggacg tggaggtaaa cgtaccgcca gaagtggtca
      301 gttcaggcgg gcagtcagcg cagccctgta ctcagtgctg cgtttatcag gataaaaatt
      361 attctgaagg ggcggtaatt aaagccgaag gaatactgct gcaatgccag cgggatgaca
      421 aaacccttag cactaatccg ctggtctggc ggcgcgtaaa gccataaacg cctctaataa
      481 tggaatgtca gcaggagcca gcggatattg cagcgcctct tcaggtgagc accagaccag
      541 cgcctgatgt tcatgtgcct gtaacgtccc gtggaagtcg ggtacgtgcc aggcatgaag
      601 atggataatc cggcccgaaa cttctcgctg atggctggca acatactcac ccacagttgc
      661 ttcgatgccc aattcttcgt ttaactcacg caccagcgcc tgctgctggc tttcatccgg
      721 ctcgacttta ccaccggcaa actcccataa tcccgcctga tcgctctggg cggggcgttg
      781 cgcgagtaaa attttgccat cacgttcaat gatggcggca acaacttcaa tcattttcat
      841 aaattacaca cgggcagata agcgaaaaat gccataatat ccgcactgtt gctcaatcag
      901 aaaggcttta tatttaagga gtgcctgtgc tagaccgcca tcttcatccc cggattaaac
      961 cgttgttgca tcagtgtgtg cgggttcttg ataaaccggg cattacgcca gatggtttaa
     1021 cattagtggg atttgccatc ggtgtgctgg cactgccttt tctggcgctg ggctggtatc
     1081 tggcggcgct tgtcgtcatt ttgttgaaca ggctgcttga tggtctggac ggtgcgctgg
     1141 cgcggcgcag agggcttacc gacgcgggcg gctttcttga tatttctctc gattttcttt
     1201 tttacgcgct ggtacctttt gggtttatcc tcgccgcacc gcagcaaaac gccctggcag
     1261 gcggctggct gctgtttgcg tttatcggca cgggtagcag ttttctcgct tttgctgcgc
     1321 tggcggcgaa atatcagatt gataatcccg gttatgcgca taaatcgttt tattatctgg
     1381 ggggattaac cgaaggcaca gaaacgatct tactgtttgt gctgggatgt ttattccctg
     1441 catggtttgc gtggtttgcg tggatcttcg gtgcgttgtg ctggatgacg acctttacgc
     1501 gggtgtggag tgggtatctg acgctgaagt cgctccagcg tcagtaattg caatgttatt
     1561 tgctgctgtc cgggccgcgt tcaccggttg ctaccggatt ttttggatcg ctgctccatt
     1621 cgtaccagcc gccgtcgtaa acggagacgt tattccagcc cattgctcgt gcgtacataa
     1681 aggtttcgga cgcgcgccag ccggtgccgc agtagaatga aacttgctga tctggtttga
     1741 tattccatgc tttccacata gcggtaatat catcggcgct acgcatggtg ccatccggat
     1801 tatggaaatc ttccatatgc gtcgagtcgc taccagcgtg tccccaacgt gctccggcta
     1861 tttcaccttt tggtttaata tagctgtaac cgctggtcgt accgataaat tctggccacg
     1921 aacgaatgct caccagcgat gcatcctggc gatgcagcag tccacgggcc tgttccatat
     1981 cgagcatcaa ctgcggttgt gccgggatct tcacgccgaa atccggttcc gctttcactt
     2041 tcggtggcgt accgcgctca acgggcagac ccgcgtcgga ccaggtttgc cagccgccat
     2101 ccagcaggcg cacatctttc acgccggcat aaagcataat ctgcgccaca cgcgctgcag
     2161 cgtatacgtc acgcccgtac agaatgaccg tggtgtcatg gcgaatgccg tgttttgcca
     2221 gcatcgcttt tagttgttca tcagaaactt tgttccacag cggttcgctt tccacttcgt
     2281 tggtatcgat gtagtcagca ccgggaatat ggctgataag gtaaagctta ggagcgcccc
     2341 tggccgcttc aatgactttc cagtcaccgg caggtttcgc cgtaacctct ttaccttgtt
     2401 gcaggtcgtg cagccattgc ggataaacca gctgctcaaa gtgcggcagt ttttgtagac
     2461 gggaaggttc gcttagcgcg tcactcagaa tggagatatg cgtaaaacct gctttttgca
     2521 gtcgcgtttt gacggcgtcg acatctttgc cattaccgta cagcgccacc ggggcatcgg
     2581 ctttcaggtt atgttgcttg atccacgcgt tgagctgttc ggtgctcatt ttgtcaagcc
     2641 agctagcaga gaggtttaag gcggcaggtt catgaccaga agggccattt aaggtttgtg
     2701 gccagccgtt ataaaacgcg ctggagcgag tatctatcgc tttgccattt tgttgttgaa
     2761 gctggtcaag tgtaagaggc ttcgccagtt cagcggccca cgaagaagcg caagccagcc
     2821 ctaaagccat tgccagcgcg gtcatttgag aaacacgttt catcgaaaat cccgacattg
     2881 tagttttgag tgcgccattc tgaactgacg cattgagaaa aactttgcgt tatcgcagtt
     2941 tgttgtaatt ttctgaccac tgcgccatat ccagaacaga actatcagca ggaacatcct
     3001 ggagatcgtg cgttacctga acgacgggga tcgccagttc gcgaacttcg ctgaacaccc
     3061 actggcgaaa attatcgcgc agagccacat caagacggct gaatggctca tccagtagca
     3121 acgcttttgg ctgagcgaga agggcgcgaa gcagagcaac gcgcgctcgc tgaccgccag
     3181 acaaagtagc aggatcctga tggaaagctc cgtcaaggcc tgaacgctca agtgcatcat
     3241 tcacggcatt acgtcgggta ttccccttaa gtgtcgccgg tagcgccagc agtaaatttt
     3301 gcccgacact gaactggtca aataacagtg catcctgaaa aagaatgcca atctgacgct
     3361 gtgcggtggg tagcatgtca atccgttgct cattgagcca tagctcacct gtacaagaaa
     3421 actgtccggc cagtgcacca atcatccatg aaaacagagt ggattttcca cagccagacg
     3481 gccccattaa cgtgacaatg tcacctttat ccaccgtaaa gttaacgttt gtcagcaagc
     3541 aactttctgg taaacgtagc gaaacatttt tcacgcagag cattagcgga gtccttgtct
     3601 gacataacct acccattttg cgactaacgc ggtcagggca aaaataataa gcggtaatag
     3661 cagttgccat aaagccaggg cggcgagaat accgttgctg ccgccgctgc ttaatgccac
     3721 cgcctcagtg gtgagcgtcg gaaaacgccc cgcgcccagc cacagcgtcg gcatatactg
     3781 cgcaatactg actgaaaatc ccaccgcgaa ggcaatcagc gtagggcgta gcataagcgg
     3841 acatttcacg taaaagaaga ttttggcccg cgaccagccc agtgtttgtg caatcaaaat
     3901 taaccgtgaa tcaatgcgct gccaggcggg ttgcaggata aacagcatcc acggcattac
     3961 ccacagcaga tgcccccaga ccaccgccgt ccagcttcca tccatgttca gccatagcgc
     4021 cagcgtgtac tggcccgcca ccagcggcag tgccggtaat aaaatcggca accatagcca
     4081 caactggcga cgctgtggcc cccattccag ccacagcagt aacaggagca aagcgatgaa
     4141 tgtcgccacc agccccattg tcaggctgtt gatgagcgct tcactattga tcgtcgactg
     4201 atccgcgagg atcgccagca gaaccacaca cagcacactg gtcaagggta aaaaactcgc
     4261 cagcgtaatg cctggcaata aaggcgtggc aggcttacga acgccatcta cgcggggaat
     4321 agtacgccgc caggtacacc atagcagata gcccagcaaa acgtaggcgg cgagtaacaa
     4381 catcagcagc aggctggcga gtgcgccttt cgtttgttga tcggcgtcgc cctgagttaa
     4441 ccactgccag ctaattactg ccagcgtcgg tggattacct ggcccaagaa taatcgccac
     4501 atctacgacc gacagcgacc aggcaacaat cgccagcatc gccattgcca gcgcaggcgc
     4561 gacggagggc aacagcagcc agttcaggca ttgccagcgg ctgtagccca gcgaatccag
     4621 cacgatgact tgctgtaaaa gccgtttttc actcaatacc gctgctaaga tccacagcag
     4681 aaatgcgctt tctttcaccg ccagcgtgaa gcccagccca atacccaatt tgtccattgg
     4741 cggagtgaaa tacgggaaat agtcataaag cagccctccg tcagcaaaga gcagaagggc
     4801 gctggtggca aaagccacat ggggaatggc gagcagccac ggcagacggg tgcacatacg
     4861 ctgccatttc ggcccaggcc acagcgccac aatcaccagt agggcaatca acaacgcccc
     4921 gaccgccgcg atggttgtcg acaccagcgt tgccagtaat gcctgcggta actgtggatc
     4981 ggcaaataac gcctgccagt gtgtcaacga caaagcaggc gatatcagcg tgagagctgc
     5041 cgggatcagc ggtgcataaa tcaccgccac catcgcccac agcaaaaaga ttaatgcgta
     5101 ccgtaacggt gtagccattc ttgttccagc gcatttaccc aacctgcgtg cggttcagcc
     5161 agtaccggcg gcagatcttg cggcattctt gattgcaatg tttcgcgctg cccatcaggc
     5221 agtttttgcg gatcgagaac agaaggatcg ccccagacag cgggatctgc tttacgcaat
     5281 tgcgcatcgg gtgagagcaa gaaattggca actaccttcg ccgcagcact ggcattcgcg
     5341 ttggcaggaa tggtgacgaa atgcacgttg ccaatcatcc cctcgcgaaa gccaaaactg
     5401 taactgcttg caggcaaatc gccgctggcg attttttgct gcgcatgcgc ggggttaaag
     5461 gtcagcgaca ggcgaaatgt tccggctttc agcagagcat ccatccgcgc gggcgaaggc
     5521 gggaaatcct ttccttcgcg ccacaaatac ggatgcagcg catcaagata ttgccacaag
     5581 ggagcagtga cacgggcgaa agtcgcatcg ttcggcgctt catttaatgc ggcgggatcg
     5641 ggcgtcagca taatcagcaa ctgttcaaga aacgccgtgc cggtaaagtc cggtgggcgc
     5701 ggataggtaa ccgtgccggg attggcttta gcaaactcca gtaaggcttg cggcgtttgt
     5761 ggtggctgtg gcgtaacatc gcggcgggcg ataaatgtca gttgtgcgcc cccccagggc
     5821 gattcagccc cttctgtagg cactgaaaaa tcttcccgca ccggcagctg tgtgtcgaca
     5881 tagcgccagt tgggcagagt ctctgcccag tcggtttgca gtaaattggc ctcttttaag
     5941 gtgcggaagt tttcgccgtt cacccagagc agatccaccg agccgcccgt tttacgtccg
     6001 gcagcagctt cggtctgaat gcgcttcacc gcgtctgcgg catccgccag acgaacaatc
     6061 ttcaggttta tagcgtaatg ggttttcatc tcgccgctaa cccagtcgag atagcggtta
     6121 attgcggtat cgccgcccca ggcgttaaac cagacagttt gccctttggc ctcattttta
     6181 atttcttgcc agtcactggc atgtgttgcc agagaaaaca gcgataacaa tcccagcaac
     6241 cacccacaat ggcgcatcat ctctccttaa gaacgttttt ccggaatacg gcacagcccc
     6301 tgagcgggaa tgtccatggc agaattaaaa cgggctgaca gattttgcag gccgatcaat
     6361 gccgtcagtt cggtgagctc ctgagcgtca aaatgcgcag ccagtcgggt acgcagggca
     6421 tcatcgaccg ttggcggcgt tacgcttgcg gcttcggcgt actccagcgc cagccgttct
     6481 tcatcgctaa agagcgggct ttgccgccag tcagccactg ccagcagttt atcgctgctg
     6541 ccggtgcgct cggcgacttt catactggtg atatccacac aaaactcaca caggcacatt
     6601 tgcgcaatgc gcgcgctgac aagcgatcgt actaccggat cgagcggtga gcgtttgcgc
     6661 tccagccagc caacaaacat cgacaccaga taaaagataa acgggatccg cccccaccag
     6721 cgaatggggc ttagcacctc gccgtaatgg cgacgatgca gccacgcctg cggacgtaaa
     6781 atgaaaggaa ttttgctaag cggcggtaaa cccatcaggc attcctcttc tggcgctcaa
     6841 gccatatttt cttcgccata aaaatcacca cggtcagggc aaacagaata aacagtccgg
     6901 tgacaaacca gaaggtaccg cctgttaaca tgctgcccgc ccaggaataa acaatagtcg
     6961 ccggtaactg accaagcccg gtggcgataa aaaacgagcg aaaacgtatt gaagtcaaac
     7021 cggcagcata gctgattgga tcgaaaggga caaaaggcaa taaccgacag accaggatgg
     7081 tgtgtttgcc gtagcgagtg aaaaagccgt ccatactgtc aagtacggtt ttgccggtta
     7141 atttttccac cacttcgcgg cccatcactc tggcgataaa aaagcacagt gccgcgccgg
     7201 ccatcgaact ggtccacgac agcgagccgc cccagaacgc gccaaacagc gacgcattgg
     7261 caaaggtgat caaaaacgca ggtagcggtg cagcaatggc ctgtaaaatc atcaacaaga
     7321 acgagacaac tgccgccagt gcgccgtaag actgaataaa gcgttctata ccctgttggt
     7381 ccacggcggc aaacgccgca acgctgcgat tgataaactc atggacgccg ggaagcagcg
     7441 cccatgccag cagcatagcg aacaacagga tgatgagggt tatacggtag taccagattt
     7501 tacgcgattg catcttcatc atcctttatc ctggctttat tcggtgattt taaaggtaac
     7561 cgttgccagc gtgttattcg ccgggagaac cgaggcattg cctttgaatt taacttcacc
     7621 tcgtttttca accgcaccat aagtgtatgt tgcattgctg acgatgccga ccgggcagct
     7681 atccagacac accaggcaac ccgttttctt ctcttctgct gccgttaaat taccgccaaa
     7741 gcgcatgtcc agtttcttgc cattactgtc aacaatcact tcatcgaagg aatacgtttt
     7801 tgccgcccct tgccagttga ccgaaatatc cagtttgctg cctgtgacat gagtcgtttc
     7861 tttattatcc atcgtcatgt tttcgcccgg tgtaccacct gcctctttca gggcttcata
     7921 aaatgctttc ggggtcgcat aacccataaa cagcgattta tgtccgttgg agccatcttt
     7981 aaacacaata ccgtgacgag tgtcgtcggt gaggaaacga ccattaattt gcaccagcat
     8041 ggttaccgta tggttattgg catcgacgcg cagcggttgg ctgttcgaaa gtccgtcgta
     8101 ttccactttt gccgccgcat tctctttttg atcgcagccg ctaagcccag caacagccag
     8161 taagcagagt gcagtcagtc cttttttcca tgacactgaa taatgttgca acatcgttct
     8221 atccttcatt ttttgggtga gtaagtgggt tgcggcgcga agcagacaga tccacatgtt
     8281 tgtgtcgggc gtagagtttt gcgagctgga cgagaataaa cagcgccagg cccgccagac
     8341 agagttgtaa aataaagcgc agcgtaatgc cttcattggc gagatcgctt gccatcacgg
     8401 tataaataac aataccgggc agggtcgtga gtgccgaaat aagggtataa ggccagaagg
     8461 cgattgtggt taatccgtaa gcgtaatttt gaatattgta aggaaacaac gggattaagc
     8521 gggtcagaat aagaaaatca ataccgttac gcgcaatgcc tttttcaatg gcctggaagg
     8581 tatggctatg gccaacgtat ttcagcagta aatcacgtcc catccagcgc gccagcagga
     8641 atgagcacga ggaggccagc gtggcggcaa ttaatgagag tagtgtccct aagaacgggc
     8701 caaaaactat tcctcccgcg atcaccaata tgcttcctgg taacagaaag agggtggcaa
     8761 tgatgaataa cagaatatag aggctataac cgaacaatcc gctctggcga atgagtgtct
     8821 gtaagtgggg taaatcggtg agcagatcga ataaaccgaa agcgtggata gcgtaaatgg
     8881 ccagcgcaaa aataagacag gcaaaaagaa atttacgctc agcgttcatc gtggagtttc
     8941 ctatatcaaa cttgataata gagaagtggt cagacaacta aaaacgcagt gacggtataa
     9001 caaaggacgg caggcaacaa atcattactg acagtttgtt gatttttctc aaggttaatt
     9061 ctcctgaccc agtttgagcc aggagagctg ctaaattagc ggcggaaggt cgcccagacg
     9121 ggggcgtgat cggacggttt ttccatgctg cggatttcat agtcgatgcc ggtttctacg
     9181 caacattctg ccagcggttg gctggcgagc agcaggtcga tgcgcagacc acggttatcg
     9241 tcaaaacctt ttgagcggta atcaaaccat gagaaacgat ctgctgtttg cggattcgca
     9301 tggcggaagg tatcaaccaa tccccagctc atcagtctct ccatccattc gcgttcctcc
     9361 ggaaggaaag aacattttcc ggtacgcagc cagcgcttac ggttctcttc gccaatgcca
     9421 atatccaggt cgccaggact gatattcata tcgcccataa tcagcaccgg attttcacgc
     9481 ttgagttcgg tttccaggta gttttgcaga ttctgataaa actgcgcctt tgccgggaat
     9541 tttatcggat ggtcgcggct ttcaccctgc gggaagtaac cgttgatcac ggtgacattg
     9601 cccagcggcg aggggatttc cgccataata atccgccgct gcgcctcttc ttcgtcaccg
     9661 ggaaagccgc gacgcacggc aatcggcgtc tctttggtca gcagcgccac gccataatgg
     9721 cctttctgcc cgtgataaaa cacgttgtag ccgagcttcg ccacctcttc gaggggaaac
     9781 atatcgtcat gaacttttgt ctcctgcagg ccaatcacat ccggttggtg cttttcgacg
     9841 atggcttcaa gctggtgggg tctggcgcgc aggccattga tattaaaaga gacaaatttc
     9901 atagtcgctg ccatttattc agataaagag tgcgtggatg gtagcagaat ttcgctttgc
     9961 ttaccgcctg ttgttaagtg atttgatcga tggttgttaa tgatgtcaac gatggcgcaa
    10021 aaaatgcccg cttttggtgc gcgctgcgtc agaatggcgc agtaatttcc agtaaatttc
    10081 gtcaatgatc acaattccag aat
//
LOCUS       DBFIFI010000157        10053 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.157, whole genome shotgun sequence.
ACCESSION   DBFIFI010000157 DBFIFI010000000
VERSION     DBFIFI010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10053
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.157"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..234
                     /locus_tag="N7B39_003633"
     CDS             <1..234
                     /locus_tag="N7B39_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HGU2423544.1"
                     /translation="GKLIDTGFCIFALSKLAMALSSTLDSIPLSMQRQFPDLTPRHLD
                     HLKTLIAKGANQCARAGDKLPDLLDEYIRATTE"
     gene            206..2135
                     /locus_tag="N7B39_003634"
                     /pseudo
     CDS             206..2135
                     /locus_tag="N7B39_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
     gene            2119..2325
                     /locus_tag="N7B39_003635"
     CDS             2119..2325
                     /locus_tag="N7B39_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HGU2423545.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLDELN
                     RAINDAESVLGTTRRRRRPLGVRL"
     gene            2322..3914
                     /locus_tag="N7B39_003636"
     CDS             2322..3914
                     /locus_tag="N7B39_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU2423546.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYNGGGAGFGGQMAEWLPPAQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETAAK
                     SFVDEVEAAWSEYAEGMSGEIDVEGKRTFTEFIREGVGVHAFNGEIFVQPVWDTETTQ
                     LFRTRFKAVSPKRVDTPGHGMGNRFLRAGVEVDRYGRAVAYHICEDDFPRSGSGRWER
                     IPRELPTGRPAMLHIFEPVEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVKAMYA
                     ATIESDLDTEKAFEYIAGAPQGQKDNPLINILEKFSSWYDTNNVTLGGVKIPHLFPGD
                     DLKLQTAQDSDNGFSALEQALLRYIAAGLGVSYEQLSRDYSKVSYSSARASANESWRY
                     FMGRRKFIAARLATQMFSYWLEEALLRGIIRPPRARFDFYQARSAWSRAEWIGAGRMA
                     IDGLKEVQESVMRIEAGLSTYEKEPALMGEDYQDIFRQQVRESAERQKAGLSRPVWIA
                     QAYQQQIAESRRPEEETTPRET"
     gene            3904..5409
                     /locus_tag="N7B39_003637"
     CDS             3904..5409
                     /locus_tag="N7B39_003637"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HGU2423547.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADSQVRGVLLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMSHVSYAGHLAQAGVDITLIYSGAHKVDGN
                     QFEALPAEVRQNMQQRIDAARRMFAEKVAMFTGLSVDAVTGTEAAVFEGQSGIDAGLA
                     DELVNASDAISVMATALNSNVRGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAAAAPQQPVASTQSEADRIMACEEANGREQLAATLAAMPE
                     MTVEKARPILAASPQADAGPSLRDQIMALDEAKGAEAQAEQLAACPGMTVESARAVLA
                     AGSGKAEPVSASTTALFERIMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            5446..5793
                     /locus_tag="N7B39_003638"
     CDS             5446..5793
                     /locus_tag="N7B39_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HGU2423548.1"
                     /translation="MVTKNITEQRAEVRIFAGNDPAHTATGSSGISSATPALTPLMLD
                     EASGKLVVWDGQKAGSAVGILVLPLEGTETVLTYYKSGTFATEAIRWPESVDEHKKAN
                     AFAGSALSHAALP"
     gene            5851..6102
                     /locus_tag="N7B39_003639"
                     /pseudo
     CDS             5851..6102
                     /locus_tag="N7B39_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
     gene            6099..6839
                     /locus_tag="N7B39_003640"
     CDS             6099..6839
                     /locus_tag="N7B39_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU2423549.1"
                     /translation="MPLEPVKGAGTTLWVYNGQGDAYANPLSDDDWQRLAKVKDLTPG
                     EMTAESYDDNYLDDEDADWTATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFESGDVR
                     AYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERSKITPVSAI
                     KVTPTSGTVAKGKTTTLTVSFEPESATDKTFRAVSADPSKATISVKDMTITVNGVATG
                     KVQIPVVSGNGQFAAVAEVTVTEAGAAG"
     gene            6853..7284
                     /locus_tag="N7B39_003641"
     CDS             6853..7284
                     /locus_tag="N7B39_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HGU2423550.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEQAESSGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTQSPSMNEAVMKIEQEVLTTWPADAIARAED
                     VVLCLSGMSGAVRPDTDITEVAKNNTLTDDDFSAGKSSTAS"
     gene            7311..7724
                     /locus_tag="N7B39_003642"
     CDS             7311..7724
                     /locus_tag="N7B39_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HGU2423551.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWHRFYRTHYFQDTQLDMHFSGLTY
                     AVLSLFFCDPDMHPSDFSLLVPRHEEEQVERPDEDKMLMQKAAGLAGGVRFGGDGGRD
                     ILSSADVADVMVDDAALMMASAGIPGGVRYVPAGW"
     gene            7705..>10053
                     /locus_tag="N7B39_003643"
     CDS             7705..>10053
                     /locus_tag="N7B39_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU2423552.1"
                     /translation="MSQPVGDLVIDLSLDAVRFDEQMSRVRRHFSGLDTDVRKTASAV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGVTSLAVATGALVYAWYQGDSTLSAFNKTLVLSGNQS
                     GLTADRMLTLSRAGQAAGLTFNQARESLAALVNAGVRGGEQFDAINQSVARFASASGV
                     EVDKVAEAFGKLTTDPTSGLIAMVRQFRNVTAEQIAYVAQLQRSGDEAGALQAANDIA
                     TKGFDEQTRRLKENMGTLETWADKTGKAFKSMWDAILDIGRPESSADMLASAQKAFDE
                     ADKKWQWYQSRSQRRGKTASFRANLQGAWNDRENARLGLAAATLQSDMEKAGELAARD
                     RAERDASQLKYTGEAQKAYERLLTPLEKYTARQEELNKALKDGKILRADYNTLMAAAK
                     KDYESTLKKPKSSGVKVSAGERQEDQAHAALLALETELRTLEKHSGANEKISQQRRDL
                     WKAENQYAVLKEAATKRQLSEQEKSLLAHKDETLEYKRQLAELGDKVEYQKRLNELAQ
                     QAVRFEEQQSAKQAAISAKARGLTDRQAQRESEAQRLRDVYGDNPAALAKATSALKNT
                     RSAEEQLRGSWMAGLKSGWGEWAESATDSFSQVKSAATQTFDGIAQNMAAMLTGAEAD
                     WRGFTRSVLSMMTEILLKQAMVGIVGRIGSAIGGAFGGGASASSGTAIQAAAANFHFA
                     TGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRG"
BASE COUNT         2300 a         2459 c         3208 g         2086 t
ORIGIN      
        1 ggaaaactga ttgacaccgg gttctgtatt tttgccctca gtaagctggc aatggcgtta
       61 tccagtacgc ttgattccat ccctttatcc atgcagcgac agtttcctga tttaacaccg
      121 cgccatcttg accatctgaa aacccttatt gctaaggggg caaatcagtg tgcgcgggca
      181 ggggataaat taccggattt acttgatgaa tatatcagag caacaactga ataatatgat
      241 gagcgctgtc acaactgcat tacagcccct gataagggca ttgccggtga cgccagttga
      301 atgggctgat caaaattatt atctgcctaa agaatcttca tatggtgagg gcgaatggaa
      361 aacgctgcca ttccagatcg ccatcatgaa cagtatgggg aatgatcaga tccgcactgt
      421 taatctgatt aaatctgccc gtgttggcta tacaaagatg ttgctggggg gggggcgggt
      481 attttattga gcataaatcc cgaaacagtc tgctttttca gcccacggat tctgccgctg
      541 aagattttat gaagtctcac gtggaggcga cgattcggaa cgtgccatgc ctgaaagacc
      601 tttccccatg gctgggtcgt aaacatcgtg acaatactct cacgctgaaa cgcttttcat
      661 cgggcgtcgg tttctggtgc ctgggcggcg ctgccgccaa aaactaccgt gaaaaatccg
      721 ttgacgtggt ctgctatgac gaactttcct cgttcgagcc ggatgtcgaa aaagagggct
      781 cgccaaccct gctgggggat aagcgtattg aggggtcggt gtggccaaaa tccattcgcg
      841 gctcgacgcc taaaatcaaa ggcacctgcc agatcgaaaa agccgctaac gagtcggcgc
      901 attttatgcg tttttatgtg ccctgcccgc actgtgggga ggcgcagtat ctgaaatttg
      961 gcgatgagtc cacgcctttt gggcttaaat gggagaagga cagccctgaa agtgttttct
     1021 acctctgtga acatcatggc tgcgtgatcc atcagtctga actggaccag agcaacgggc
     1081 ggtggatctg tgaaaacacg ggcatgtgga cccgtgacgg tctgacgttt ttcagcgccc
     1141 ggggtgatga aattccgccg ccgcgctcca tcatgttcca tatctggacg gcgtacagtc
     1201 cgttcaccac ctgggtacag attgtctatg actggctgga tgcactgaaa gatcccaacg
     1261 gcctgaaaac ctttgtgaac accacgctgg gcgagacctg ggaagaggcc gtgggcgaaa
     1321 aactcgatca ccaggtactg atggataagg tggtgcgtta cacggcggcg gtgcctgccc
     1381 gggtggttta tctgacggcg ggcattgact cgcagcgaaa ccgttttgag atgtatgtct
     1441 ggggatgggc tccgggagag gaagcctttc tggtggataa aatcatcatt atggggcgtc
     1501 ctgatgagga agagacgctg ttacgtgtgg atgcggcgat caacaaaaaa taccgccatg
     1561 cggatggcac cgaaatgact atttcccgtg tctgctggga caccgggggg atcgatggtg
     1621 aaattgttta tcagagatca aaaaaacacg gtgttttccg ggtgctgccg gtaaaaggcg
     1681 catctgtcta tggcaagccg gtgatcacca tgccaaaaac ccgcaatcag cggggcgtgt
     1741 atctgtgtga agtgggaacg gacaccgcaa aagaaattct ctatgcccgt atgaaagccg
     1801 atccctcgcc tgcggatgaa gccacgtcgt atgccatccg ttttcctgat gatccggaga
     1861 ttttttcgca gacagaggcg cagcaactgg tggcggaaga gctggtggag aagtgggaaa
     1921 aaggaaagat gcgtctgctg tgggataaca aaaagcggcg taacgaagcg ctggactgcc
     1981 tggtgtatgc ctacgcggca ttacgtgtgt ccgtgcaacg ctggcagctt gatctggctg
     2041 tactggcaaa atcccgggaa gaagagacga cccggccaac cctgaaagaa ctggcagcga
     2101 agctgtccgg aggagtgaat ggttacagtc gctgaactgc aggcgctgcg tcaggcgcgc
     2161 cttgatttat taaccggtaa acgggtggtg tctgtccaga aagatggtcg cagaattgaa
     2221 tatacggcgg cttctctgga tgagcttaac cgggcgatca atgatgcgga gtcggtactg
     2281 gggacaaccc ggcgtcgccg tcgtccgctg ggagtgaggt tatgaaacga acgcctgtcc
     2341 tgattgatgt gaacggcgtt ccgcttcgtg agagtctcag ctacaacggg ggcggtgcag
     2401 gatttggcgg gcaaatggcg gagtggttgc caccggcgca gagtgccgat gcggccctgc
     2461 tgcccgcgtt gcgtctgggg aatgcccggg cagatgatct ggtgcgcaat aacggaatag
     2521 cggccaatgc ggtggcactg cataaggatc acattgtcgg gcatatgttt cttatcagct
     2581 accgtccgaa ctggcgctgg ctggggatgc gggagaccgc agcaaaaagc tttgtcgatg
     2641 aggtggaggc ggcctggtcg gaatacgccg aagggatgtc tggcgagatc gacgtggaag
     2701 gaaaacgcac gttcacggaa tttatccgtg aaggtgtggg cgttcatgcg tttaacggcg
     2761 aaatctttgt gcagccggtc tgggatacgg aaaccacgca gttattccgt acgcgtttta
     2821 aagccgtgag tccgaaacgg gtggacacgc caggacacgg tatggggaac cgttttctgc
     2881 gggccggggt ggaggtcgat cgatatggcc gtgccgttgc gtaccatatc tgtgaggatg
     2941 attttcctcg ctccgggagt ggacgatggg aacggatccc gcgtgaactt cccaccgggc
     3001 gtccggccat gctgcatatt ttcgagccgg tggaggacgg gcagacccgt ggggccaacc
     3061 agttttacag cgtcatggaa cggctgaaga tgctcgattc cctgcaggca acacagcttc
     3121 agtcggccat tgtgaaagcc atgtatgcag cgacgattga aagtgacctt gataccgaaa
     3181 aggcctttga atatatcgcc ggtgcgccgc aggggcagaa ggataatccg cttattaata
     3241 ttctggagaa gttctccagc tggtatgaca cgaataacgt gacgctgggt ggtgtcaaaa
     3301 ttccgcacct tttccccggg gatgatctga aactacagac tgcgcaggat tcagacaatg
     3361 gattttcggc gcttgaacag gcgctgctgc ggtatatcgc cgccggtctt ggcgtttcct
     3421 acgaacagtt gtcccgtgat tactcgaagg tcagttattc aagtgccagg gcctctgcca
     3481 atgagtcgtg gcgctatttt atggggcggc gaaaatttat tgcggcccgg ctggccacgc
     3541 agatgttttc ctactggctg gaagaggcac ttcttcgggg gattatccgt ccgccacggg
     3601 cgcgttttga tttttatcag gcgcgatcag cctggtcacg ggcagagtgg attggtgccg
     3661 gaagaatggc cattgacggg ctcaaggagg ttcaggaatc ggtgatgcgc attgaggccg
     3721 gactgagcac gtatgagaaa gagccggcgc tgatgggcga ggattatcag gacattttcc
     3781 gccagcaggt cagggaatct gcagagcggc aaaaagccgg actctcacgt ccggtgtgga
     3841 tagcgcaggc gtatcagcag cagatagcgg agagtcgcag gccggaagag gagacaacac
     3901 cccgtgagac gtaatctttc acacattatt gccgcagcat tcaatgaacc gctgcttctg
     3961 gagcccgcct atgcgcgggt tttcttttgc gcgctcgggc gcgagatggg ggcagcaagt
     4021 ctttcggtac cacagcagca ggtacagctt gatgctcccg gaatgctggc tgaaacggac
     4081 gagtacatgg ccggaggtaa acgaccggcc cgtgtttacc gggtggtgaa cggtattgct
     4141 gtactgccgg tgaccggcac gctggtgcac cggctggggg gtatgcggcc attttccgga
     4201 atgacaggct atgacggcat tgtcgcctgt cttcagcagg caatggcgga tagccaggtg
     4261 cggggcgtac tgctggacat tgacagtccg ggcgggcagg ccgccggcgc gtttgactgc
     4321 gctgacatga tttaccgcct ccgtcagcag aagccggtct gggcactgtg caatgacacg
     4381 gcctgttctg cagccatgct gctggcgtcg gcctgctccc gacggctggt tacccagaca
     4441 tcccgtatcg gctccattgg cgtgatgatg agccatgtca gctatgccgg tcatctggcg
     4501 caggccggtg tggatatcac gctgatttac tcaggggcgc acaaggtgga tggcaatcag
     4561 tttgaagcct taccggcaga ggttcgccag aacatgcagc agcgcattga tgcggcgcgc
     4621 cggatgtttg ccgaaaaagt ggccatgttt accggtctgt ctgttgatgc cgtcacggga
     4681 acagaggccg ccgtttttga aggtcagtcc ggcattgatg ccgggctggc ggatgaatta
     4741 gtcaatgcgt cggatgccat cagtgtgatg gccacggcgc tgaacagtaa tgtcagagga
     4801 ggcactatgc cgcaattaac tgcaacggaa gccgccgcgc aggagaacca gcgagtgatg
     4861 gggatcctga catgccagga agcgaaagga cgtgaacagc ttgccacgat gctggcagga
     4921 caacagggca tgagcgttga acaggcccgg gcgattctgg ccgcggcggc accgcagcag
     4981 ccggtggcat ccacgcagag tgaagccgat cgcattatgg cgtgtgaaga agcgaacggt
     5041 cgtgaacaac tggcggcaac gctggcggcg atgccggaga tgacggtgga aaaagcccgc
     5101 ccgatcctgg ctgcttcacc gcaggcggat gccggaccct cactccgtga tcagatcatg
     5161 gcactggatg aggcaaaagg ggctgaggcg caggctgaac agctggctgc ctgcccggga
     5221 atgactgtgg agagcgcccg ggctgtgctg gctgcgggat caggtaaggc agaaccggtc
     5281 tctgcatcca caaccgccct gtttgaacgc atcatggcga accattcacc ggctgcggta
     5341 cagggtggcg tgccacagac gtcagcagac ggtgatgcgg acgtgaaaat gctcatggcc
     5401 atgccatgaa gtcagtgctg accatcaaca ggaggttttt acaatatggt aacgaaaaac
     5461 atcactgaac agcgtgcgga agtacgtatt tttgccggta atgatccggc tcataccgcc
     5521 acaggcagca gcgggatttc ctcggcaaca ccggcactga cgcccctgat gctggatgaa
     5581 gccagcggga aactggtggt ctgggacgga cagaaagccg gtagtgcagt tggcatactg
     5641 gtactgccgc ttgaaggcac agagacggta ctgacctatt acaagtcggg gacctttgcg
     5701 acggaggcaa tccgctggcc tgaaagtgtg gatgaacaca aaaaggcaaa tgcctttgcc
     5761 ggcagtgccc tgagtcacgc ggcgctgccg taacacgtta tcaggccacc gcggtggcct
     5821 gactgatttc tgaatgaaag gaactgattt atgggattgt ttacgacccg ccagttactc
     5881 ggttataccg aacaaaaagt gaaatttcgt gcgctgtttc tggagctgtt tttccgccgt
     5941 acggtgaatt tccataccga agaggtgatg ctggacaaaa ttaccggaaa aacgccggtg
     6001 gcggcctatg tctccccggt tgttgaagga aaagtgctgc gtcatcgcgg tggtgaaacc
     6061 cgcgtgttac gtccgggcta cgtcaagccg aaacacgaat tcccctggag ccggtaaaag
     6121 gagccggtac caccctgtgg gtttataacg gtcagggtga cgcctatgca aacccgttgt
     6181 cagacgatga ctggcagcga ctggctaagg tgaaggatct gacgccgggc gagatgacgg
     6241 cagaatccta cgatgataac tacctggatg atgaagacgc ggactggacc gcgaccgggc
     6301 aggggcagaa atctgcaggt gataccagtt ttacgctggc ctggaaaccg ggagaggaag
     6361 gccagaaagg gcttataggc tggtttgaaa gcggcgatgt ccgggcctat aaaatccgtt
     6421 ttccgaatgg cacggtggat gtgtttcgtg gctgggtcag cagtatcggt aaggccgtga
     6481 cggcgaaaga agtgatcacc cgcacggtga aagtcactaa cgtgggtaaa ccttctgtag
     6541 cggaagaacg cagcaaaatt acgccggtca gtgcgattaa ggtgacgccg acatccggta
     6601 cggtggcaaa agggaaaaca accaccctga cggtttcttt tgagccggaa agtgcaacag
     6661 acaagacgtt cagagcggtt tccgccgatc cgtcgaaagc caccattagt gtgaaagata
     6721 tgacaattac ggtaaacggc gtggcgacag gtaaggtgca gatccctgtg gtgagcggaa
     6781 atggtcagtt cgccgcagtg gctgaagtca ccgttactga agcgggcgct gcagggtaaa
     6841 cggaggtcat acatgtttct gaaaacagaa caatttgaat ataacggtgt gtctgtcacg
     6901 ctttccgaat tgtctgcgct gcagcgtatc gagcatcttg ccctcctgaa acggcgtgca
     6961 gaacaggcag aatccagcgg caacctgcag gtaagcgtgg aagatctcgt cagaaccggc
     7021 gcgtttctgg tggcgatgtc cctgtggcat aaccatccac agaaaacgca gtcaccgtca
     7081 atgaatgagg ccgtgatgaa gatagagcag gaagtgctca ccacctggcc tgccgatgcc
     7141 attgcccggg cggaagacgt ggtgttgtgc ctgtccggga tgagcggggc tgttcgtccg
     7201 gatactgata ttactgaagt ggcgaaaaat aacacgctga ctgatgatga tttttctgcg
     7261 ggaaagtctt cgacggcgag ctgaactttg ccctcagact ggcgcgtgag atggggagac
     7321 ccgactggcg cgccatgctt gccgggatga catccaccga atatgccgac tggcaccgtt
     7381 tttaccgcac gcattatttt caggataccc agctggatat gcatttttcc gggctgacgt
     7441 acgctgtact cagcctgttt ttttgcgatc cggatatgca tccctctgat ttcagtctgc
     7501 ttgtcccccg gcatgaggaa gagcaggtgg agaggccgga tgaggacaaa atgctgatgc
     7561 agaaagcggc aggacttgcc ggaggcgtcc ggttcggtgg ggacggaggg cgcgatattt
     7621 tatcgtctgc ggatgtggcg gatgtcatgg tggatgatgc cgcattaatg atggcttcag
     7681 cggggattcc gggaggtgtg agatatgtcc cagccggttg gtgatcttgt tattgacctg
     7741 agtctggatg ctgtccgttt cgatgagcag atgagccggg taaggcgtca tttttcaggt
     7801 ctggataccg acgtcagaaa aaccgccagt gctgttgaac agggcctgag ccgccaggcg
     7861 ctggctgcac aaaaagccgg gatttccgtc gggcagtata aagcggccat gcgaaccctg
     7921 cccgcacagt ttacggatat cgccacgcag cttgccggtg gtcagaatcc ctggctgatc
     7981 ctgctgcaac agggcggtca ggtgaaggac tccttcggcg ggatgatccc catgttcagg
     8041 gggcttgccg gtgcgatcac cctgccgatg gtcggggtca cctcgctggc ggtggcgaca
     8101 ggtgcgctgg tgtacgcctg gtaccaggga gattccacgc tttcagcgtt taataaaacc
     8161 ctggttcttt ccggtaatca gtccggactg actgccgatc gcatgctgac gctctcaaga
     8221 gccgggcagg cagcagggct gacgtttaac caggcgagag agtcactggc agccctggtg
     8281 aatgccggtg tgcgtggtgg tgaacagttt gatgccatca accagagtgt cgcgcgtttt
     8341 gcttctgcat ccggtgtgga ggtggacaag gttgcagagg ctttcggaaa actgaccacc
     8401 gaccctacgt cggggctgat tgcgatggtg cgccagttcc gtaacgtgac ggcagagcag
     8461 attgcgtatg ttgcgcagct gcagcgttcc ggtgatgagg ccggggcctt acaggcggcg
     8521 aacgatatcg ccacgaaagg ctttgatgag cagacccgtc gcctgaaaga aaacatgggg
     8581 acactggaga cctgggcgga taaaaccggg aaggcattca aatcgatgtg ggatgccatt
     8641 ctggatatcg gtcgtcctga gtcctcagcg gatatgctcg ccagtgcaca gaaggcattt
     8701 gatgaggcgg ataaaaaatg gcagtggtac cagagccgga gccagcgccg gggaaaaacc
     8761 gcctctttcc gggccaacct tcagggcgca tggaatgacc gggaaaatgc ccgtctgggg
     8821 ctggcagcgg ccacgctgca gtcggatatg gaaaaagccg gtgaactggc cgccagggac
     8881 cgggccgaac gggacgcatc acagctgaag tataccggag aggcgcagaa ggcgtatgag
     8941 cgtctgctga cgccgctgga gaaatatacc gcccgtcagg aagaactgaa taaggccctg
     9001 aaagacggga aaatcctgcg ggcggattac aacacgctga tggcggcggc gaaaaaggat
     9061 tatgaatcga cgctgaaaaa gccgaagtcg tcaggagtca aagtgtcagc cggtgagcgt
     9121 caggaagacc aggcgcatgc tgccctgctg gcgcttgaaa ccgagctcag gacgctggaa
     9181 aaacacagcg gtgcgaatga gaaaatcagc cagcagcgtc gcgatttatg gaaagcggaa
     9241 aatcagtatg cggtcctgaa agaggctgcc acgaaacggc agttatctga gcaggaaaaa
     9301 tccctgctgg cgcataaaga cgagacgctg gagtacaaac gccagctggc tgagctgggc
     9361 gacaaggttg aataccagaa acgcctgaat gagctggcac agcaggcggt gcggtttgaa
     9421 gagcagcaga gcgcgaagca ggccgccatc agcgcaaaag cccgcggtct cactgaccgt
     9481 caggcgcagc gggagtctga agcgcagcgt cttcgggacg tgtacggtga taatccggct
     9541 gcgctggcga aggccacatc ggcactgaag aacaccaggt ctgcggagga gcagcttcgt
     9601 ggaagctgga tggccgggct gaagtccggc tggggcgagt gggcggaaag tgcgacggac
     9661 agtttttcgc aggttaaaag tgctgccacg cagacctttg acggtattgc acagaatatg
     9721 gcggcgatgc tgaccggtgc agaggcagac tggcggggat tcacccgttc ggtgctgtcc
     9781 atgatgacag aaatcctgct taaacaggcc atggtgggca ttgtcgggcg tatcggcagc
     9841 gccattggcg gtgctttcgg tggtggtgca tctgcttcct cggggacggc cattcaggct
     9901 gcggcggcga acttccattt cgcgaccgga ggatttacgg ggacgggcgg caaatatgag
     9961 cctgcgggga tagttcaccg cggggagttt gttttcacga aagaggcaac cagccggata
    10021 ggtgtgggga atctttaccg tctgatgcgc ggc
//
LOCUS       DBFIFI010000158         9942 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.158, whole genome shotgun sequence.
ACCESSION   DBFIFI010000158 DBFIFI010000000
VERSION     DBFIFI010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9942
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.158"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            126..341
                     /gene="bdm"
                     /locus_tag="N7B39_003644"
     CDS             126..341
                     /gene="bdm"
                     /locus_tag="N7B39_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HGU2423553.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            443..580
                     /gene="sra"
                     /locus_tag="N7B39_003645"
     CDS             443..580
                     /gene="sra"
                     /locus_tag="N7B39_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HGU2423554.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            737..2434
                     /gene="maeA"
                     /locus_tag="N7B39_003646"
     CDS             737..2434
                     /gene="maeA"
                     /locus_tag="N7B39_003646"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HGU2423555.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDRDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            2568..3578
                     /gene="adhP"
                     /locus_tag="N7B39_003647"
     CDS             2568..3578
                     /gene="adhP"
                     /locus_tag="N7B39_003647"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HGU2423556.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            3724..4008
                     /locus_tag="N7B39_003648"
     CDS             3724..4008
                     /locus_tag="N7B39_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HGU2423557.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAAKTVDVSRLRRLVAQ"
     gene            complement(4236..4889)
                     /gene="fdnI"
                     /locus_tag="N7B39_003649"
     CDS             complement(4236..4889)
                     /gene="fdnI"
                     /locus_tag="N7B39_003649"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HGU2423558.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4882..5766)
                     /gene="fdnH"
                     /locus_tag="N7B39_003650"
     CDS             complement(4882..5766)
                     /gene="fdnH"
                     /locus_tag="N7B39_003650"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HGU2423559.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQSGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5779..8826)
                     /gene="fdnG"
                     /locus_tag="N7B39_003651"
     CDS             complement(5779..8826)
                     /gene="fdnG"
                     /locus_tag="N7B39_003651"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(8239..8241),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HGU2423560.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKEHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLAGQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            9058..9939
                     /gene="yddG"
                     /locus_tag="N7B39_003652"
     CDS             9058..9939
                     /gene="yddG"
                     /locus_tag="N7B39_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HGU2423561.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         2402 a         2619 c         2522 g         2399 t
ORIGIN      
        1 atataaaaat catcaaccag gactaatctt aacaacgaag cggcaaatat ttgccatgct
       61 gaatgtgctt tataaatctg cgatccgtag cagacactat aaatacacag acacggagaa
      121 tcactatgtt tacttattat caggcagaaa attcaacagc agaacccgct ctggttaatg
      181 ccattgagca aggccttcgg gcagagcacg gtgttgtcac tgaagatgac attctcatgg
      241 agctgaccaa atgggtggaa gcttctgata acgacatcct cagtgatatc taccagcaaa
      301 cgatcaatta tgtggtcagt ggccagcacc ctacgcttta agatgctatg cttgatcggc
      361 aacctaattt aggggtttag cacgtgtttc ttcgctacgg cgatgttgtc cttaaaacta
      421 gcaacaggat tgaggagtta aaatgaaatc gaaccgtcag gcacgtcata ttcttggact
      481 ggaccataaa atatctaacc agcgcaaaat agttaccgaa ggtgacaaat ccagcgtggt
      541 aaataaccca accggcagaa aacgccccgc tgaaaagtaa ttcataacca tcagtcctca
      601 atgacgatta aacaccattg cctgcgcaat ggtgtttttg tttttatctg ctttatactt
      661 ggggccgacg ccctggcggt aaagcaaaga cgataaaagc cccccaggga tggatattca
      721 aaaaagagtg agtgacatgg aaccaaaaac aaaaaaacag cgttcgcttt atatccctta
      781 cgctggccct gtactgctgg aatttccgtt gttgaataaa ggcagtgcct tcagcatgga
      841 agaacgccgt aacttcaacc tgctggggtt actgccggaa gtggtcgaaa ccatcgaaga
      901 acaagcggaa cgagcatgga tccagtatca gggattcaaa accgaaatcg acaaacacat
      961 ctacctgcgt aacatccagg acaccaacga aaccctcttc taccgtctgg taaacaatca
     1021 tcttgatgag atgatgcctg ttatttatac cccaaccgtc ggcgcagcct gtgagcgttt
     1081 ttctgagatc taccgccgtt cacgcggcgt gtttatctct taccagaacc gccacaatat
     1141 ggacgatatt ctgcaaaacg tgccgaacca taatattaaa gtgattgtgg tgactgacgg
     1201 tgaacgtatt ctggggcttg gtgaccaggg catcggcggg atgggcattc cgatcggtaa
     1261 actgtcgctc tataccgcct gtggcggcat cagcccggcg tatacccttc cggtggtgct
     1321 ggatgtcgga acgaacaacc aacagctgct taacgatccg ctgtatatgg gctggcgtaa
     1381 tccgcgtatc actgacgacg aatactatga attcgttgat gaatttatcc aggctgtgaa
     1441 acaacgctgg ccagacgtgc tgttgcagtt tgaagacttt gctcaaaaaa atgcgatgcc
     1501 gttacttaac cgctatcgca atgaaatttg ttcctttaac gatgacattc agggcaccgc
     1561 ggcggtaaca gtcggcaccc tgatcgcagc aagccgcgcg gcaggtggtc agttaagcga
     1621 gaaaaaaatc gtcttccttg gcgcaggttc agcgggatgc ggcattgccg aaatgatcat
     1681 cgcccagacc cagcgcgaag gattaagcga ggaagcggcg cggcagaaag tctttatggt
     1741 cgatcgcttt ggcctgctga ccgacaagat gccgaacctg ctgcctttcc agaccaaact
     1801 ggtgcagaag cgcgaaaacc tcagtgacag ggataccgac agcgatgtgc tgtcactgct
     1861 ggatgtggtg cgcaatgtaa aaccagatat tctgatcggc gtctcaggac agaccgggct
     1921 gtttacggaa gagatcatcc gtgagatgca taaacactgt ccgcgtccga tcgtgatgcc
     1981 gctgtccaac ccgacgtcac gcgtggaagc cacaccgcag gacattatcg cctggactga
     2041 aggtaacgcg ctggtcgcca cgggcagtcc gtttaatcca gtggtatgga aagataaaat
     2101 ctaccctatc gcccagtgta acaacgcctt tattttcccg ggcatcgggc tgggtgttat
     2161 tgcttccggc gcgtcacgta tcaccgatga gatgctgatg tcggcaagtg aaacgcttgc
     2221 ccagtattcg ccgctggtcc tgaacggcga aggtctggta ctaccggaac taaaagatat
     2281 tcagaaagtc tcccgcgcaa ttgcgtttgc ggttggcaaa atggcgcagc agcaaggcgt
     2341 ggcggtgaaa acgtctgccg aagctttgca acaagccatt gacgataatt tctggcaagc
     2401 cgaataccgc gactaccgcc gtacctccat ctaagcctgc gcccggtagt gaaggctacc
     2461 gggctatttc cctctccctt tttcagatct catccatact gggtagtggc gaataaatct
     2521 catttgcctc acctgctatg cagaacatca tccgaaaagg aggaactatg aaggctgcag
     2581 ttgttacgaa ggatcatcat gttgacgtta cggataaaac actgcgctca ctgaaacatg
     2641 gcgaagccct gctgaaaatg gagtgttgtg gtgtatgtca taccgatctt catgttaaga
     2701 atggcgattt tggtgacaaa accggcgtaa ttctgggcca tgaagggatt ggtgtggtgg
     2761 cagaagtggg tccaggtgtc acctcattaa aaccaggcga tcgtgccagc gtggcgtggt
     2821 tctacgaagg atgcggtcat tgcgaatact gtaacagtgg taacgaaacg ctctgccgtt
     2881 cagttaaaaa tgccggatac agcgttgatg gcgggatggc ggaagagtgc atcgtggtcg
     2941 ccgattacgc ggtaaaagtg ccagatggtc tggactcggc ggcggccagc agcattacct
     3001 gtgcgggggt caccacctac aaagccgtta agctgtcaaa aattcgtccc gggcagtgga
     3061 ttgctatcta cggtcttggc ggtctgggta acctcgccct gcaatacgcg aagaatgtct
     3121 ttaacgcgaa agtgatcgcc attgatgtca atgatgagca gttaaaactg gcaaccgaaa
     3181 tgggtgcaga tttagcgatt aactcacgca ccgaagacgc cgccaaaatt gtgcaggaga
     3241 aaaccggtgg cgctcacgct gcggtggtaa cagcagtagc taaagctgcg tttaactcgg
     3301 cagttgatgc tgtccgtgca ggcggtcgtg ttgtggctgt cggtctgccg ccggagtcta
     3361 tgagcctgga tatcccacgt cttgtgctgg atggcattga ggtggtcggt tcgctggtcg
     3421 gcacgcgcca ggatctaact gaagccttcc agtttgccgc cgaaggtaaa gtggtgccga
     3481 aagtcgccct gcgtccgtta gcggacatca acaccatctt taccgagatg gaagaaggca
     3541 aaatccgtgg ccgtatggtg attgatttcc gccgctaaga ggcctttgct gcgactccca
     3601 tgttcgggtc gcagcatcgc acactctcca acatgaaatg gctaaatgga ttattgttgc
     3661 gtaggaagga acaataatga tagtgttccc agagaaggaa cgctactgag agtaaagaca
     3721 ctaatgaaaa tggccaatca tccccgcccg ggggacatta ttcaggaatc actggacgaa
     3781 cttaatgtca gcctgcgcga gtttgccaga gcaatggaaa ttgctccctc aacggcaagc
     3841 cgattgctga ccggaaaagc agctttgacg ccagaaatgg caattaaact ttccgtggtg
     3901 atcggcagtt cgccgcaaat gtggctgaat ctgcaaaacg cctggagtct ggcagaggct
     3961 gcaaaaacgg tggatgtgtc gcgattacgc cgtctggttg cgcaataaaa aatggcgcct
     4021 tttacagcgc catttgaaac tgatgacaaa cgcaacattg cctgatgcgc tacgcttatc
     4081 aggcctacac gttcactgca atctattgga tttgaacggt ttgtaggccg gataaggcgt
     4141 tcacgccgca tccggcatga acaaagccta cgttgtctac aatatgaaaa tggcgccttt
     4201 tacagcgcca ttttcagctt aaagttcaaa agagattata tcccttcttc actctctttt
     4261 ttcgcttctg ccttctcgat ttcacgatac cagcgcggat ggtgtttctt cgcccagcga
     4321 cggctcacct tcccttcgat catcccttta atcgatcctt tcacccagaa tgccatatac
     4381 atatggatca ggatggcgtg gatcaggatg atacccgcag ccgcgtggat cagcaggctg
     4441 tagcgaacaa cctgcatcgg gaagtactgc gcaaagtacg gacgccagat aatcaccccg
     4501 gtcaccagca gcacgaaaat catgctcatg atcgaccaga acatcatctt ttgcccggcg
     4561 ttgtacttac cgacatccgc cactttatgc tcattgcctt tcaatacttc gacaatgttt
     4621 aacagccacg gaatatcttt cttatccggg atgttgtgat gcacaaaacg cacaaacata
     4681 aacatcagtg cgacgaaaat cgcaatgccg aagaatgggt gcaaaatgcg tcccatctgc
     4741 ggcgtaccga aggtttgcgt cagccattgc agcgtcggga agaagaacga aatcccggac
     4801 agcgccacca ggaagaagca aatcaccacg gtccagtgac aggcgcgatc aataaatttg
     4861 gtgcgcacaa tcattttcga cttactcatg atgatcctcc tcgtcatcgt ccacttcctt
     4921 attcgggcca ataccgatgt agtggaaaat caaccctgca aaagtggcaa taaagccagc
     4981 cgctgccagc ggtttcaacg cgcctttcca caggcttacc gaggtgtcga tcttcggatc
     5041 tttcggcaga ccgtgataca gctccggctg atcggcgtga tgcagcacgt acataacgtg
     5101 cgtaccaccg accccttccg ggttgtagac gccagcatgt tcgtaaccac gcgctttcag
     5161 tttcgccacg cgctgttccg ccagctccag catctccttc ttggtgccga agtggatagc
     5221 cccggtcgga caggttttca cacaagccgg ttcctggccg acgctgacgc gatcgacgca
     5281 gagcgtgcat ttatataccc ggttatcctc tttgttgagg cgcggaatat taaacggaca
     5341 cccggcaatg cagtaaccac agccgatgca gttttccgac tggaaatcga caatcccgtt
     5401 agcgtactga atgattgcac cggcagacgg gcacgccttc aggcagcccg gatcttcaca
     5461 gtgcatacag ccatctttac ggatcagcca ctccagcttg ccgctctgtt cggtttcgct
     5521 aaagcgcatc accgtccagg acttggcgct cagatcggcg gggttatcgt aaaccccgac
     5581 gcagtgcccc acttcatcac ggatgtcgtt ccactccgaa cacgccacct gacaggcttt
     5641 acagccgata caggtggaaa cgtcgataag ttttgcgact tctgctttgt aatcacgcac
     5701 ctgagaaggc ggcgtgatgg agttagttgc ggaccttttg ataatgtcct gcgtttccat
     5761 agccatctgt tcgccccctt acgccttctc gatgttgact aagaacgctt tatattccgg
     5821 cgtttgcgag tttgcatcac cgacattcgg cgtcagagtg ttagctatat aacctttacg
     5881 cgcgacaccc tcaaagcccc agtggattgg aatacccacc gtttcaacct gctgaccatt
     5941 tacattcagc ggtttcagac gacgcgttac cacagccacc gcgcggataa agccacgctt
     6001 gcttgagaca gtgacacgat cgccattatt aatgcctttc gccgccgcca gcgtttcgct
     6061 gatttccaca aactgttccg gctgagcaat tgcgttgagc aacgcgtgct tggtccaggt
     6121 gtggaagtgc tcggtcagac gataggtcgt acccacatac gggaactgct cttttttacc
     6181 catccgcagc gcgtcttgtt catacagacg aaccaccggg ttagaaacca cgttcggatg
     6241 cagcgggtta gttcccagcg gcgtttcaat tggctcgtag tgttccggga acggaccttc
     6301 cgccattttg ttgatggcaa acaggcgtcc catcccttcc ggctgcatga taaacggccc
     6361 ggttggcgta cccggtgcgg cattgccgaa gtcagggata tcgttacccg tccacttgct
     6421 gccgttccac tggatcagca tccgtttcgg atcccacggt ttaccgttga tatctgccga
     6481 agcacggttg tagagtacgc gacggttaag cggccacgcc caggcccatc ccagcgtatt
     6541 ccccagaccg gacgggtcgg agttatcgcg gttagccatc tggttgccct gctctgtcca
     6601 gctaccggtg tagatccagc aagaagatgc ggttgtaccg tcatcacgca gatgcgcaaa
     6661 gctactcagc aactgacctt tcttcgcaat cagcacgcca ttagcatcat agagatcttc
     6721 cagcgcatag ccgttgttct ctttagctac ttcgtcagat tgcggttcgt gcggctgctt
     6781 gtagttccag ctcatcttca tcagcggttc tacgccttta ccaccttcag cctggtacag
     6841 ctcgcgcaga tgatggtaga tacccgccag aatttcgccg tcgttacgcg cttcgcccgg
     6901 cgcgtcctga cctttccagt gccactgcaa ccagcgaccg gagttagcga tagaaccatc
     6961 ttcttctgca aagcaggtcg aaggcagacg gaatacttca gtctgaatag acgccggatc
     7021 gacatcgttc gactcgccgt ggttctgcca gaaggtagag gtttcagtca ccagcggatc
     7081 gataaccacc atgtacttca gcttgctcag gcagctcacc actttgtttt tgtccgggaa
     7141 ggacgcaacc gggttaaagc cctggcagaa ataaccggtg actttgcctt catccatcat
     7201 gttgaaatac ttgatgacgt cgtaggtctg gtcccacttc ggcagccagt catagcccca
     7261 gttgttctct ttctgcgcgg catcgccata gaaagatttc atcaggctaa cgaagaactt
     7321 cggatagttg ctccagtagt tcacctgacc agccagcgtc gctttcggcg tgttcgcttc
     7381 cagatacgac tgcaaatcaa cctgtttttc tgacggcagc gtcagataac ctggcaggct
     7441 ggtagagagc agacctaagt cagtcagacc ctgaatgttg gagtgaccac gcaatgcatt
     7501 cacgccgcca ccggccatac ccatgttacc gagcagcaac tggatcatcg ccatagtacg
     7561 gatgttctgc gcacccacgg tgtgctgcgt ccagcccagc gcgtacagga aggtggttgt
     7621 gcgatccggt gcgctggtgg aggccagcac ttcacacact ttcaggaagt cggcttttgg
     7681 cgtaccacag atgttttcaa caacgtccgg cgtgtagcgg gaaacgtgct ctttcagcag
     7741 gttccacaca cagcgcggat gagtcagtgt ttcatcgcgt tttgcatagc cgttttcatc
     7801 gaactgatag ttccaggacg acttatcgta ctggcgtttt tcagcgtcgt agccgctgaa
     7861 cagaccgtct tcgaaagcaa aatcatcacg caccagcagg ctggcgttgg tgtaatgctt
     7921 aacgtattcg gcgttgattt tgttgttttc gatcaggtag cgcaaaacgc cagacaggaa
     7981 cgtaatgtcc gtaccggaac gaataggcgc gtagatatcc gccacagaag cggtacgcgt
     8041 aaaacgggga tcgacaacga tcaaggttgc atcgttgttg tttttcgctt ccatcgccca
     8101 gcggaaaccg acgggatgcg cttcagcagc gttaccgccc attaccatta cgacgttagc
     8161 gtttttgata tccacccagt ggttggtcat cgcaccgcga ccaaatgttg gagcaagact
     8221 tgctaccgtt ggtccgtgtc agacgcgcgc ctggttgtct accgccagca tcccgaggga
     8281 gcgggcaaat ttctgggtca gcatcccggt ttcgttgctg gcaccggaag cacacagcat
     8341 accggtagaa agccaacggt ttaccgttac gccctgctcg ttcttttcaa taaagttagc
     8401 gtcacggtca gctttcatca gcttcgcaat acgggagaat gcttcttccc agctaatgcg
     8461 ctgccatttg tcagaacctg gcgcacgata ttccgggtag cgcagacggt tttcgctgtt
     8521 gacgtaatcc agcaaaccgg cccctttcgg gcacagcgcc ccacggctta ccggatgatc
     8581 cgggtcacct tcaatgtgat aaatcgcttc tctggcgttt ttcgcgccat cacccaggct
     8641 atacatcaat agcccgcaac ctacggaaca gtatgtgcag gtgttacgga tctctttagc
     8701 gcgtaataat ttgtagtttc gcgcctgagc cagtgcttgc ttcggggcaa agcccaatgc
     8761 cgctactgtt gttccagcca taccgcccgc gcagatttta aaaaattgtc tgcgactgac
     8821 gtccattgct ttcctctttt ttcaggggta ttactgcgcg aggaaactaa cagtaaaatt
     8881 ctgaatcttt ttgcgcgaaa tcaaaaagaa cacgtgtttg ccacaaaata atccgcctgc
     8941 gcaaattgac ctacctcaat agcggtagaa aaacgcacca ctgcctgaca ggccagttaa
     9001 aaaaatgcta taaaattcag cttaattttt aacggcaaga gagacaaaac agcgagcatg
     9061 acacgacaaa aagcaacgct catagggcta atagcgatcg tcctgtggag cacgatggta
     9121 ggattgattc gcggtgtcag tgaggggctc ggcccggtcg gtggcgcagc tgctatctat
     9181 tcattaagcg ggctgctgtt aatcttcacg gttggatttc cgcgtattcg gcaaatcccg
     9241 aaaggctatt tactcgccgg gagtctgtta ttcgtcagct atgaaatctg tctggcgctt
     9301 tccttagggt atgcggcgac ccgtcatcag gcgattgaag tgggtatggt gaactatctg
     9361 tggcccagcc tgacaattct ctttgccatt ctgtttaatg gtcagaaaac caactggctg
     9421 attgtaccag gattattatt agccctcgtc ggcgtctgct gggtgttagg cggtgacaat
     9481 gggttacatt atgatgaaat catcaataat atcaccacca gcccattgag ttatttcctg
     9541 gcgttcattg gtgcgtttat ctgggcagcc tactgcacag taacgaataa atacgcacgc
     9601 ggatttaacg gaattaccgt ttttgtcctg ctaacgggag caagtctgtg ggtttactat
     9661 tttctcacgc cacaaccaga aatggtattt agcacgccag tcatgattaa actcatctct
     9721 gcggcattta ccttaggatt tgcttatgct gcatggaatg tcggtatact gcatggcaac
     9781 gtcaccataa tggcggtagg ttcgtatttt acgcccgtac tttcctcagc gcttgcagcc
     9841 gtgctgctca gcgccccgct gtcgttctcg ttctggcaag gcgcgctgat ggtctgcggc
     9901 ggttccctgc tctgctggct ggcgacacgt cgtggctaaa at
//
LOCUS       DBFIFI010000159         9934 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.159, whole genome shotgun sequence.
ACCESSION   DBFIFI010000159 DBFIFI010000000
VERSION     DBFIFI010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9934
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.159"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(259..2832)
                     /gene="clpB"
                     /locus_tag="N7B39_003653"
     CDS             complement(259..2832)
                     /gene="clpB"
                     /locus_tag="N7B39_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HGU2423562.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(2962..3693)
                     /gene="yfiH"
                     /locus_tag="N7B39_003654"
     CDS             complement(2962..3693)
                     /gene="yfiH"
                     /locus_tag="N7B39_003654"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HGU2423563.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLNLTGEPYASKRADASYSNTPGRVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAEASTAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3690..4670)
                     /gene="rluD"
                     /locus_tag="N7B39_003655"
     CDS             complement(3690..4670)
                     /gene="rluD"
                     /locus_tag="N7B39_003655"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HGU2423564.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKLCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            4805..5542
                     /gene="bamD"
                     /locus_tag="N7B39_003656"
     CDS             4805..5542
                     /gene="bamD"
                     /locus_tag="N7B39_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HGU2423565.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5813..6154
                     /gene="raiA"
                     /locus_tag="N7B39_003657"
     CDS             5813..6154
                     /gene="raiA"
                     /locus_tag="N7B39_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HGU2423566.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6257..6382
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6258..6305
                     /gene="pheL"
                     /locus_tag="N7B39_003658"
     CDS             6258..6305
                     /gene="pheL"
                     /locus_tag="N7B39_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HGU2423567.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6404..7564
                     /gene="pheA"
                     /locus_tag="N7B39_003659"
     CDS             6404..7564
                     /gene="pheA"
                     /locus_tag="N7B39_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HGU2423568.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7607..8728)
                     /gene="tyrA"
                     /locus_tag="N7B39_003660"
     CDS             complement(7607..8728)
                     /gene="tyrA"
                     /locus_tag="N7B39_003660"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HGU2423569.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPALRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8739..9809)
                     /gene="aroF"
                     /locus_tag="N7B39_003661"
     CDS             complement(8739..9809)
                     /gene="aroF"
                     /locus_tag="N7B39_003661"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HGU2423570.1"
                     /translation="MQKDALNNVHITDEQVLMTPAQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
BASE COUNT         2512 a         2646 c         2335 g         2441 t
ORIGIN      
        1 atacgctttc ccgctacaga gtcaagcatt tatttttgct ttctctgccg gaattctcag
       61 gagaaccccg ctgacccggc ggcgtgtttg ccgttgttcc gtgtcagtgg tggcgcatta
      121 tagggagtta ttccggcctg acaagcgaaa aatataaaaa ctttatcgtt cgctcatttt
      181 tcaggcaaaa catcttaaat atagtctttt ccgtctaact tatagacaaa aacgagcccc
      241 gaagggctcg ttttatcatt actggacggc gacaatccgg tcttcattaa cttccaggcg
      301 aataacttta cccggaacca attcaccaga cagtatttgc tgtgccaacg ggttttcgat
      361 ctgctgctgg atagcacgtt tcaaaggacg tgcaccatag actggatcgt aaccgttctc
      421 gctcagcagt ttcagcgcct cgtcagaaat gtggatttca taaccacgtt cttccagacg
      481 tttgtacaga cgtttcaact gaatctgcgc aatcgaggca atgtgctgtt cgcccagcgg
      541 atggaagacc accacttcat cgatacggtt aatgaactcc ggacggaagt tatggcttac
      601 cacaccgagc accagctctt tcatgtgcgc ataatccagt tcaccgaagc gttcctgaat
      661 cagatcggaa ccgaggttag aggtcataat gacgaccgta ttacggaagt cgaccgttct
      721 cccttgcccg tcagtcagac gcccatcatc cagtacctgc aacagaatgt tgaagacatc
      781 cggatgcgct ttttccactt catccagcag gatgacggaa tacggacgac gacgcaccgc
      841 ttcggtcagg tagccacctt cttcataacc gacatatccc ggaggcgcac caaccagacg
      901 agacaccgag tgtttctcca taaactcgga catatcgata cggaccatcg cctcgtcgct
      961 atcaaacata aagttcgcca gcgccttaca aagctctgtt ttccccacac cagttgggcc
     1021 gaggaacagg aatgaaccaa tcgggcgatt tggatccgcc agccccgcac ggctacgacg
     1081 aatagcgtta gataccgcat caaccgcttc gttctgacca attacgcgat ggtgcagttc
     1141 ttgctccata cgcagcagtt tttcgcgctc gctttccatc atgcgagaaa ccggaatccc
     1201 cgtccaacgc gccagcactt cagcaatttc ggcgtcggtc actttattac gcaacagacg
     1261 catagttttg ccttcgagct gcgttgcggc ttccagttgc ttttccagtt ccgggatttt
     1321 gccgtattgc agttcagaca tccgcgccaa gtcacccaca cggcgagcct gttcaatagc
     1381 gattttcgcc tgttccagtt ccgctttaat ggtctgcgta ccagaaagcg atgccttctc
     1441 tgctttccac tcttcttcta actcggagta ctgacgttct ttgtcgctca gttcttcgtt
     1501 gagcatatcc agacgttttt tactggcttc atcagactct ttcattaacg cctgttgttc
     1561 cagtttgagc tggatgatac ggcgatcgag tcggtcgagt tcttctggtt ttgagtcaat
     1621 ctgcatacga atgctggatg ctgcttcatc gatcaggtcg atggctttat ccggcagctg
     1681 acggtcagca atgtagcgat gagacaacgt cgccgctgca acaattgccg ggtcagtaat
     1741 ttgcacatgg tggtgcaatt cgtaacgttc tttcaggcca cgcagaatcg caatggtatc
     1801 ttcaacagaa ggctcggcaa caaacacttt ctggaaacga cgttccagcg cagcatcttt
     1861 ttcgatgtac tggcgatatt cgtcaagcgt cgtggcacct acgcagtgca attcaccacg
     1921 cgccagcgcc ggtttcagca tgtttccggc gtccattgcg ccatcggctt tacccgcgcc
     1981 gaccatggta tgtaattcgt cgataaacag gatgacgttg ccttcctgtt tggcaagatc
     2041 gttaagcacg ccttttaaac gttcttcaaa ctcaccgcga tatttcgccc cagccaccag
     2101 cgcgcccata tccagcgcca gtacccggcg gcctttcaac ccttccggca cttcgccgtt
     2161 gataatacgc tgcgccagac cttcaacgat ggcagtttta ccgacgccgg gttcaccaat
     2221 cagtaccggg ttatttttag tacgacgttg cagcacctga atggtacggc gaatttcttc
     2281 atcacgacca atcaccggat cgagtttgcc ctgttcggct cgttcggtaa ggtcgatggt
     2341 atattttttc aaagcctgac gttggtcttc agcaccttga tcgttcacgc tttcacctcc
     2401 acgcatttgt tcaatcgctt gagtaatgtt ggcggtggtc gcccctgctg ctttcaggat
     2461 gtcggccagc gtgccgcgag actcaagtgc cgccagaacg aacagttctg acgagataaa
     2521 gttatcacca cgtttttgcg ccagcttgtc gcaaagatta agaacgcgca ccagatcctg
     2581 tgatggctgg acatcaccac cagtaccttc aacctgcggt aaacgattta atgcctgatt
     2641 gatatctgtg cgcaactggc cagcatttat gccagcggat gttaataaag gactaaccga
     2701 acccccttcc tgattcagca gggcgctcat taaatgaagt ggttcgataa attggttgtc
     2761 gtgcccaagt gcaagtgatt gggcatcggc aagagcaagc tggaatttat tagtaagacg
     2821 atccagacgc ataactcctc ccataacgga tcaaagttgc tactggagat taaatgaggt
     2881 catccctcaa ttattcaagg ttattgacca gattaatgtg aaaagaaaat cacgcgtacc
     2941 ggatcgtctt gattctttag gttatatcag ccaaatgaaa cttgccatac gaccggtggt
     3001 cttgtcgcga cgataagaga agaaagtctc attttccgta tatgtacaac ggtcgccgcc
     3061 gaaaatttgc tcaacaccca cgttcgccag acgctgccgg gcaagctgat aaatgtccgc
     3121 cagatactta tcaccatgct gaatgaaagc tgtactcgct tcggcgtcta ctgccataaa
     3181 cgcctcgcga acctccgccc ccacttcaaa cgcgcgtggg ccaattgccg gccctaacca
     3241 ggcgagaata ttttctggat tatcagcaaa acaggaaact gtctcttcca gcacgcctgc
     3301 gcacagtcca cgccagccag catgagcggc ggcgacttcc gttcccgctc gattgcaaaa
     3361 cagcacaggg aggcagtcgg cagtcatcac tgcgcaaacc ctgccgggcg tattgctata
     3421 agaggcatct gctcgttttg aggcataagg ttcgccagta agattaagca catctttgcc
     3481 gtgtacctgc tcaagccaga ccggtttaga aggcaaattg cccgcagcaa aaagtcgctt
     3541 gcgattctcc tcaacgtgat ccgggttatc gccacaatgg gcaccgaggt tgagcgagtc
     3601 atacgggggc aagctcacgc cgccgatacg agtagagcta caggccgcaa caccttttgg
     3661 cagcggccac tgcgggacaa tcagcttact cataaccagt ccacttcatc tttatgctct
     3721 tcgaaatcgg cgcgcatcac ctcaatcagc tccaccatat cttgtggaat aggcgcatgc
     3781 cattccattt cgataccgga gatcgggtga taaagacgca gcatggttgc atgaagcgcc
     3841 tggcggtcaa atttacgcag cgtggagata aacgcttccg aagcaccttt tggcgggcgc
     3901 ggacggccac cataaaccgg atcgcccacc agcggatgag tgatatgggc catatgcacg
     3961 cggatctggt gcgtacgtcc agtttccaga cgcaaccgca gacgcgtgtg cacacggaag
     4021 tgttccatga tgcgatagtg agtcaccgcc ggtttgccca tcggatgcac cgccatgtga
     4081 gtacgtttgg tcgggtggcg gctgattggt tcatctactg taccgccagc ggtcatatga
     4141 ccaattgcca ccgcttcata ctcacgagta atttcacgcc tttgcaaaga ttcgactaaa
     4201 cgcgtctgag ccggaacggt ttttgccaca accatcaggc cagtggtgtc tttatccaga
     4261 cgatggacga tgcctgcacg cggtacatca gcaatgggcg gatagtaatg aagtaacgca
     4321 ttcagtaccg tgccatccgg gttacccgcg ccaggatgta ccaccagatc gcgcggttta
     4381 ttaatgacga tgatatcttc atcttcatag acgatatcca gcgggatatc ctgcggttca
     4441 aaacgcgctt cttcttcaat ctcagcgttg atggcaacct gctcgccacc caatactttt
     4501 tctttcggct tatcacaaag tttgccgtta accagcacgc gctggtcgag gatccattct
     4561 tttattcgcg aacgtgaata atccgggaac atttcggcca aagcctgatc taagcgttga
     4621 ccgagttggt tttcggacac cgttgcagtg agctgtactc gttgtgccat atatactgct
     4681 tcttcgttta tcgttggggt tttacggctt tgccgtttaa tatagtgtgc tattgtagct
     4741 ggtcttaacc gggagcagga acagagaatc tcccgtatta cattttgagg aaagtcaaaa
     4801 cgtcatgacg cgcatgaaat atctggtggc agccgccaca ctaagcctgt ttttggcggg
     4861 ttgctcgggg tcaaaggaag aagtacctga taatccgcca aatgaaattt acgcgactgc
     4921 acaacaaaag ctgcaggacg gtaactggag acaggcaata acgcaactgg aagcgttaga
     4981 taatcgctat ccgtttggtc cgtattcgca gcaggtgcag ctggatctca tctacgccta
     5041 ctataaaaac gccgatttgc cgttagcgca ggctgccatc gatcgtttta ttcgccttaa
     5101 cccgacccat ccgaatatcg attatgtcat gtacatgcgt ggcctgacca atatggcgct
     5161 ggatgacagt gcgctgcaag ggttctttgg cgttgaccgt agcgatcgcg atcctcaaca
     5221 tgcacgagct gcgtttagtg acttttccaa actggtgcgc ggctatccaa acagtcagta
     5281 caccaccgat gccaccaaac gtctggtatt cctgaaagat cgtctggcga aatatgaata
     5341 ctccgtggcc gagtactata cagaacgtgg cgcatgggtt gccgtcgtta accgcgtaga
     5401 aggcatgttg cgcgactacc cggataccca ggctacgcgt gatgcgctgc cgctgatgga
     5461 aaatgcatac cgtcagatgc agatgaatgc gcaagctgaa aaagtagcga aaatcatcgc
     5521 cgcaaacagc agcaatacat aacagaaacc tgaaacacaa aacggcagcc cttgagctgc
     5581 cgttttttta ttctgtcagt tgtgaaactg aagcgattta gtcactatcg atctcatcaa
     5641 atatggctcg ctttgagata ttcctcaagt aaaaaaacac ctcttcctgc gatttctcac
     5701 aaaaaagatt cgttgacaaa aagtgacaaa attatgagat ttccatcaca cattttgaca
     5761 tcaggaacgg tatgctgaat tcaccaagac gggaagacaa gaggtaaaat ttatgacaat
     5821 gaacattacc agcaaacaaa tggaaattac tccggccatc cgccaacatg tcgcagaccg
     5881 tctcgccaaa ctggaaaaat ggcaaacaca tctgattaat ccacatatca ttctgtccaa
     5941 agagccacaa gggtttgttg ctgacgccac aatcaataca cctaacggcg ttctggttgc
     6001 cagtggtaaa catgaagata tgtacaccgc aattaacgaa ttgatcaaca agctggaacg
     6061 gcagctcaat aaactgcagc acaaaggcga agcacgtcgt gccgcaacat cggtgaaaga
     6121 cgccaacttc gtcgaagaag ttgaagaaga gtagtccttt atattgagtg tatcgccaac
     6181 gcgccttcgg gcgcgttttt tgttgacagc gtgaaaacag tacgggtact gtactaaagt
     6241 cacttaagga aacaaacatg aaacacacac cgtttttctt cgcattcttt tttaccttcc
     6301 cctgaatggg aggcgtttcg tcgtgtgaaa cagaatgcga agaagaacaa taaggcctcc
     6361 caaatcgggg ggcctttttt attgataaca aaaaggcaac actatgacat cggaaaaccc
     6421 gttactggcg ctgcgagaga aaatcagcgc gctggatgaa aaattattag cattactggc
     6481 agagcggcgc gaactggccg tcgaggtggg aaaagccaaa ctgctctcgc atcgcccggt
     6541 acgtgatatt gatcgtgaac gcgatttact ggaaagatta attacgctcg gtaaagcgca
     6601 ccatctggac gcccattaca ttactcgcct gttccagctc atcattgaag attccgtatt
     6661 aactcagcag gctttgctcc aacaacatct caataaaatt aatccgcact cagcacgcat
     6721 cgcttttctc ggccccaaag gttcttattc ccatcttgcg gcgcgccagt atgctgcccg
     6781 tcactttgag caattcattg aaagtggctg cgccaaattt gccgatattt ttaatcaggt
     6841 ggaaaccggc caggccgact atgccgtcgt accgattgaa aataccagct ccggtgccat
     6901 aaatgacgtt tacgatctgc tgcaacatac cagcttgtcg attgttggcg agatgacgtt
     6961 aactatcgac cattgtttgt tagtctccgg cacgactgat ttatccacca tcaatacggt
     7021 ctacagccat ccgcagccat tccagcaatg cagcaaattc cttaatcgtt atccgcactg
     7081 gaagattgaa tataccgaaa gtacgtctgc ggcaatggaa aaggttgcac aggcaaaatc
     7141 accgcatgtt gctgcgttag gaagcgaagc tggcggcact ttgtacggtt tgcaggtact
     7201 ggagcggatt gaagcgaatc agcgacaaaa cttcacccga tttgtggtgt tggcgcgtaa
     7261 agccattaac gtgtctgatc aggttccggc gaaaacgacg ttgttaatgg cgaccgggca
     7321 acaagccggt gcgctggttg aagcgttgct ggtactgcgc aaccacaatc tgattatgac
     7381 ccgtctggaa tcacgcccga ttcacggtaa tccatgggaa gagatgtttt atctggatat
     7441 tcaggccaat cttgaatcag cggaaatgca aaaagcattg aaagagttag gggaaatcac
     7501 ccgttcaatg aaggtattgg gctgttaccc tagtgagaac gtagtgcctg ttgatccaac
     7561 ctgatgaaaa ggtgccggat gatgtgaatc atccggcact ggattattac tggcgattgt
     7621 cattcgcctg acgcaataac acgcggcttt cactctgaaa acgctgtgcg taatcgccga
     7681 accagtgctc caccttgcgg aaactgtcaa taaacgcctg cttatcgccc tgctccagca
     7741 actcaatcgc ctcgccgaaa cgcttatagt aacgtttgat taacgccaga ttacgctctg
     7801 acgacataat aatgtcggca taaagctgcg gatcctgagc gaacagtcgc ccgaccatcg
     7861 ccagctcaag gcggtaaatc ggcgaagaga gcgccagaag ttgctcaagc tgaacatttt
     7921 cttctgccag gtgcagcccg taagcaaaag tagcaaagtg gcgcagtgcc tgaataaacg
     7981 ccatattctg atcgtgctcg acggcgctaa tacgatgcaa ccgagcgccc cagacctgaa
     8041 tttgctccag aaaccattgg tatgcttccg gtttacgtcc atcacaccag accacaactt
     8101 gctttgccag gctaccgctg tccggaccga acattgggtg taaccccagt accgggccat
     8161 cgtgcgccgc cagcatggcc tgtaatggtc catttttcac tgatgccaga tcaaccagaa
     8221 tacaatcttt cggtaaaggc ggtaatttgc caataacttg ctcagtaacg tggattggca
     8281 cactaacaat caccattccg gcatcggcaa caatatcagc cgctcgatcc cagtcatgtt
     8341 gctccagaat ccgcacctga taacccgaga gtgtcagcat cttctcgaac agacgtccca
     8401 tctgaccgcc gccgccaacg ataactaccg ggcgtaacgc aggacaaagc gttttaaatc
     8461 ctttgtcgtt ttcactggag taagattcac gcatcacccg acgcaaaaca tcctcaatca
     8521 gatctggcgg tacacccaga gcttccgcct ctgcacgacg cgaggccaac atagatgcct
     8581 ctcgctccgg aacataaata ggcagtccaa agcggctttt cacctcgccc acttcagcaa
     8641 ccagttccag acgcttcgct aataaattca gcagcgcttt atcgacttca tcaatttgat
     8701 cgcgtaatgc ggtcaattca gcaaccataa taaacctctt aagccacgcg agccgtcagc
     8761 tgcccgttca gatcctgatg aatttcacgc agcaaggcat cggtcatttc ccagctaatg
     8821 caggcatcgg ttacggatac accgtatttc atttcactgc gcggttgctc ggaagactga
     8881 ttgccctcgt ggatattact ttcgatcatc agaccaataa ttgagcgatt gccatctttg
     8941 atttgagcaa ccacggattc tgccaccgca ggctgacggc gataatcttt attggaatta
     9001 ccgtggctgc aatctaccat cagagacggg cgcagtcccg cctgttccat ctctttttca
     9061 cattgcgcaa catccgcagg gctatagttc ggcgctttac caccgcgcag gatgacatgc
     9121 ccgtccggat tcccctgagt ttgtagcaac gcaacctgcc ctgcctggtt aatgccaaca
     9181 aaacggtgcg gctgggcggc ggcgcgcata gcgttaattg ctgttgccag actgccgtcg
     9241 gtgccgtttt taaagccaac cggcatggaa agcccggagg ccatttcacg gtgagtttgc
     9301 gattccgttg tacgagcacc aattgctgac cagctaaaca gatcgcccag gtattgcggg
     9361 ctattcggat ctaacgcttc cgtcgccagt ggcagtccca tattcaccag ctcaagcagc
     9421 aatttacgcg cgatctgcag cccggcttct acatcaaaag agccatccat atggggatcg
     9481 ttaattaacc ctttccagcc gacagtggta cggggttttt caaaatagac gcgcattacc
     9541 agatagaggc tatcgctgac ctctgcggca agggctttaa atcgacgagc atattccaga
     9601 gcagtttccg gatcatgaat ggaacaaggg ccacatacta ccagcagacg aggatcgcgc
     9661 ccggcgataa tatctgaaat ggttttacgc gagtcagcaa tctgggcttc ttgttgcagg
     9721 cttaatggaa aagcggcctt cagttgtgcc ggagtcatta aaacctgttc gtcggtaata
     9781 tgtacgttat tcagcgcgtc tttttgcatg atggcgatcc tgtttatgct cgtttgcgat
     9841 agttgatcct cagcgaggat gacgtaacga taacacataa agtaaagttt tcaatccata
     9901 tttcgtacat ttttctttac ataagcaatt tatt
//
LOCUS       DBFIFI010000160         9846 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.160, whole genome shotgun sequence.
ACCESSION   DBFIFI010000160 DBFIFI010000000
VERSION     DBFIFI010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9846
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.160"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..547
                     /locus_tag="N7B39_003662"
     CDS             <1..547
                     /locus_tag="N7B39_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2423571.1"
                     /translation="EGFTDEKFSSAIYNGRIFTMKRLHTLMLFLAVLFTGFNFNAEAA
                     TVKQALSCNPEAWAEQPGACPSTYELYEGDATYKAAIDKALKPVGLSGMFGKGGYMDG
                     PGGGITPVNINGTVWFQGDGCKANTCGWDFIVTLYNPKTHEVVGYRYFGLDDPAYLVW
                     FGEIGVHEFAYLVKNYVAAVN"
     gene            563..973
                     /locus_tag="N7B39_003663"
     CDS             563..973
                     /locus_tag="N7B39_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2423572.1"
                     /translation="MKTFIKTLLVAVTILFSVFATAKQVKLPNNIKYVNTTEAFSCTE
                     IDGMNCQTKNQFNYKDNSYVFVLERGGAWCYDYTVSVVNLKTGKAQMIEYGDNQLCSG
                     SNKPFFEIKNGVPTVGVIDTSGKPVGVAQDKLKI"
     gene            1194..2015
                     /locus_tag="N7B39_003664"
     CDS             1194..2015
                     /locus_tag="N7B39_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HGU2423573.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELIRHVPSIFGEDRHTSRSER
                     YAYIPTITVLENLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEII
                     LLNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPQPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETMLESLR"
     gene            2097..2576
                     /locus_tag="N7B39_003665"
     CDS             2097..2576
                     /locus_tag="N7B39_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HGU2423574.1"
                     /translation="MKTVSQNTPTIYSATTPENNPPQLVASLVPDEQRISFWPQHFGL
                     IPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMN
                     GNRAEMSPEAAGIAACLMTYSHHACRTENYAMTVHYYRLRDYALQHPEGSSIMRIID"
     gene            2591..3067
                     /gene="yeeS"
                     /locus_tag="N7B39_003666"
     CDS             2591..3067
                     /gene="yeeS"
                     /locus_tag="N7B39_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HGU2423575.1"
                     /translation="MQQISFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            3130..3351
                     /gene="yeeT"
                     /locus_tag="N7B39_003667"
     CDS             3130..3351
                     /gene="yeeT"
                     /locus_tag="N7B39_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HGU2423576.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            3425..3793
                     /gene="cbeA"
                     /locus_tag="N7B39_003668"
     CDS             3425..3793
                     /gene="cbeA"
                     /locus_tag="N7B39_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system cytoskeleton
                     bundling-enhancing antitoxin CbeA"
                     /protein_id="HGU2423577.1"
                     /translation="MSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHPDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLSSCGYVYLAVYPTPEMKN"
     gene            3882..4255
                     /gene="cbtA"
                     /locus_tag="N7B39_003669"
                     /pseudo
     CDS             3882..4255
                     /gene="cbtA"
                     /locus_tag="N7B39_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416509.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system
                     cytoskeleton-binding toxin CbtA"
     gene            4252..4446
                     /gene="yeeW"
                     /locus_tag="N7B39_003670"
     CDS             4252..4446
                     /gene="yeeW"
                     /locus_tag="N7B39_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HGU2423578.1"
                     /translation="MMTLEADSVNVQALDMGHIVVDIDGVNITELINKAAENGYSLRV
                     VDDRDSTETPATYASPHQLL"
     gene            4492..4572
                     /locus_tag="N7B39_003671"
                     /pseudo
     CDS             4492..4572
                     /locus_tag="N7B39_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4861..5007)
                     /locus_tag="N7B39_003672"
                     /pseudo
     CDS             complement(4861..5007)
                     /locus_tag="N7B39_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            5061..5243
                     /locus_tag="N7B39_003673"
     CDS             5061..5243
                     /locus_tag="N7B39_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001016346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HGU2423579.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKCWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCR"
     gene            complement(5344..5673)
                     /gene="tmaR"
                     /locus_tag="N7B39_003674"
     CDS             complement(5344..5673)
                     /gene="tmaR"
                     /locus_tag="N7B39_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HGU2423580.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(5845..6903)
                     /gene="yeeA"
                     /locus_tag="N7B39_003675"
     CDS             complement(5845..6903)
                     /gene="yeeA"
                     /locus_tag="N7B39_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HGU2423581.1"
                     /translation="MRANKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     GTWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSSLICRALRK"
     gene            complement(7102..7575)
                     /gene="sbmC"
                     /locus_tag="N7B39_003676"
     CDS             complement(7102..7575)
                     /gene="sbmC"
                     /locus_tag="N7B39_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HGU2423582.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWVDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(7694..8860)
                     /gene="dacD"
                     /locus_tag="N7B39_003677"
     CDS             complement(7694..8860)
                     /gene="dacD"
                     /locus_tag="N7B39_003677"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HGU2423583.1"
                     /translation="MKRRLIIAASLFIFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIALGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
BASE COUNT         2567 a         2519 c         2325 g         2435 t
ORIGIN      
        1 ggaaggtttc actgatgaga aattttccag cgccatttac aacggaagaa tatttacaat
       61 gaaacgttta cataccctta tgttatttct ggcggttctg ttcacgggct ttaactttaa
      121 cgcagaagcg gctaccgtaa aacaagctct tagttgtaac ccggaagcct gggctgaaca
      181 acctggagcg tgtccatcaa cgtacgagtt gtacgaaggt gacgctacct acaaagccgc
      241 tatcgacaaa gcattaaaac cggtcggact gagcggcatg ttcggtaaag gcggctatat
      301 ggatggccct ggtggaggta tcacgccagt aaacattaac ggtacagtct ggttccaggg
      361 cgacggttgc aaagccaata cctgcggctg ggactttatc gtaacactct ataacccaaa
      421 aacccatgaa gttgttggct accgttactt tggtttagat gacccggcct acctggtttg
      481 gttcggtgaa attggcgtgc atgaattcgc gtatctggtg aaaaactacg tagctgcggt
      541 taactaattt aaggagtcta tcatgaaaac ttttatcaaa actttactcg ttgctgtaac
      601 tattctgttc tctgtcttcg ctactgcgaa acaagtaaaa ctgccaaata acatcaaata
      661 cgttaatact acagaagcgt tttcctgtac cgaaattgac ggtatgaatt gccagacaaa
      721 gaatcagttc aactataaag ataacagcta tgttttcgtg cttgaacgtg gtggtgcctg
      781 gtgctacgac tacactgtct cggtagttaa cctgaaaacc gggaaagcac aaatgattga
      841 atacggggac aatcaactgt gctcaggtag caacaaaccg ttcttcgaaa tcaaaaatgg
      901 cgtaccgacg gtaggagtca tcgacacatc cggaaaacct gtcggtgtag cccaggacaa
      961 acttaaaatc taaggagtaa accatgcgtt tctctgcaca ttattaatat caaaggtaaa
     1021 tacagcccca gccattgtta ccggaaagcc gcttaatgcg tggctcgcca gtgaaaggcc
     1081 tctcagacaa taaaccttat tcatttcacc cgtcaggccg cgtcttctcc gggagacgcg
     1141 gcttttttca tttattgcac cagtaaatct taaccaccga taaggagcaa agtatgcgat
     1201 tagccagtcg ttttggttat gctgcaaacc agatacgccg tgaccgtccg ctgacacatg
     1261 aagaactgat acgccatgta cccagtattt ttggggaaga ccggcacacc tcccgcagtg
     1321 aacggtatgc gtacattccc accattaccg tcctggaaaa tctgcagcgg gaaggctttc
     1381 agccgttctt cgcctgccag acccgtgtgc gcgacccggg ccgccgggga tacacaaaac
     1441 acatgctgcg tctgcggcgg gccggagaga taaacggaga acatgtccct gaaattattc
     1501 tgctcaactc tcatgacggt acctccagct accagatgct gccgggttac ttcaggttcg
     1561 tctgccagaa cgggtgcgtc tgtggccagt ctctggggga agtgcgtgtt ccacaccggg
     1621 gaaatgtagt ggagaaagtt atcgaagggg cttacgaggt ggtgggcgtg tttgaccgga
     1681 tagaggagaa gcgtgatgcc atgcagtcgc tggtcctgcc gcaaccggca cgccaggcgc
     1741 tggcacaggc ggcactgact taccgttatg gtgacgaaca tcagcccgtc accaccgccg
     1801 acattctgac gccgcgacgc cgggaggatt acggtaagga cctgtggagc gcatatcaga
     1861 ccatccagga gaatatgctg aaaggcggga tttccggtcg cagtgcaaaa ggaaaacgta
     1921 tccatacccg ggccattcac agcattgata ccgacattaa gctcaatcgc gcattgtggg
     1981 tgatggcaga aacgatgctg gagagcctgc gctgatgccg tttccctgag tgagggaatc
     2041 attcagcttt cgttcccgaa aaacacacat ttatttgtta tttaaggagt tatctcatga
     2101 aaaccgtttc tcagaatacc cccacaattt attcagctac aacaccagag aataatccgc
     2161 ctcagttggt tgccagcctc gtccctgatg aacagcgcat cagcttctgg ccgcagcatt
     2221 ttggcctcat tccacagtgg gtcaccctgg agccccgtgt cttcggctgg atggaccgtc
     2281 tgtgcgaaga ctactgcggt ggtatctgga atctgtacac cctgaacaac ggcggggcat
     2341 ttatggcccc cgaaccggat gatgatgatg acgaaacatg ggtactgttc aatgccatga
     2401 acggtaaccg cgctgaaatg agcccggaag ccgccggtat tgccgcctgt ctgatgacgt
     2461 acagccatca tgcctgtcgt acggagaatt atgccatgac ggtccattat taccggttgc
     2521 gggattacgc cctgcagcat ccggaaggca gctccattat gcgcatcatt gactgaaagg
     2581 ggccggaata atgcaacaga tttcctttct gcccggagaa atgacgcccg gcgagcgcag
     2641 cctcattcaa cgggccctga aaaccctgga ccgccatctt catgaacccg gtgtggcctt
     2701 cacctccacc cgtgcagcac gggaatggct gattctgaac atggcgggac tggagcgtga
     2761 agagttccgg gtgctgtatc tgaacaacca gaatcagctg attgccggtg aaaccctctt
     2821 caccggcacc atcaaccgca cggaagtcca tccccgggaa gtgattaaac gcgccctgta
     2881 ccacaatgcc gctgccgtgg tactggcaca caatcacccg tccggtgaag tcacacccag
     2941 taaggcagac cggcttatca ccgaacgtct ggtacaggca ctgggcctgg tggatatccg
     3001 ggtgccggac catctgatag tcggtggcaa ccaggttttc tcctttgccg aacatggtct
     3061 gctttaaccc gtcacaacca catcacacct gttttcactt ttatcttctg tcttcagagg
     3121 tatcccatta tgaaaattat cacccgtggt gaagccatgc gtattcacca acaacatccg
     3181 acatcccgtc tttttccgtt ctgtaccggt aaataccgct ggcacggcag tgctgaagcg
     3241 tataccggtc gtgaagtgca ggatattccc ggtgtgctgg ccgtgtttgc tgaacgccgt
     3301 aaggacagtt ttggtccgta tgtccggctg atgagcgtca ccctgaactg agtgggaatt
     3361 ctgatgagca gaattatcgc cactaccgga ccattcttag ccgattttct gtaaggattt
     3421 tatcgtgtca gacacactcc ccgggacaac acttcccgac gacaatcacg accgcccctg
     3481 gtgggggctg ccctgcaccg tgacgccctg tttcggggca cgtctggtgc aggagggtaa
     3541 ccggttgcat taccttgccg accgcgccgg tatcagaggc ctgttcagcg atgcagatgc
     3601 gtaccacccg gaccaggcct ttccgctgct gatgaaacaa ctggaactca tgctcaccag
     3661 cggtgaactg aatccccgcc atcagcatac cgtcacgctg tatgcaaaag ggctgacctg
     3721 caaagccgat accctcagca gttgtggtta cgtttatctg gctgtttatc cgacgcccga
     3781 aatgaaaaat taactctcca gaatagcctt ctgctacggc ctggtgtttt caccacgcca
     3841 cttttccatt tttatatctg catatcagga aaatcttcag tatgaaaaca ttacctgtat
     3901 tacccgggca ggcggccagt tctcgcccgt ctcctgttga aatctggcag atactgctgt
     3961 cccgactgct ggaccagcac tatggcctca cactgaatga cacacctttt gccgatgaac
     4021 gtgtgattga gcagcatatt gaggcaggca tttcactgtg tgatgcggtg aactttctcg
     4081 tggaaaaata cgcgctggtg ctaccgacca gccgggattc agcgcctgta cccgctctca
     4141 gttaataaac agcatcgata tcctccgggc tcgcagggcg accggcctga tgacccgcga
     4201 caattacaga acggtaaata acattaccct gggtaagtat ccggaggcga aatgatgaca
     4261 ctggaagccg acagcgttaa cgtacaggcg ctggatatgg ggcacattgt cgttgacatt
     4321 gatggtgtta atatcactga actgattaat aaggccgctg aaaacggtta ttcactccgc
     4381 gtggtggatg accgtgactc taccgaaaca ccggcaactt atgccagccc tcaccagttg
     4441 ctgtaagaca atgcagtgat gcagggtata gtgccattaa ccacagaaac agcactgttg
     4501 tcctgtatta agcaggcaag agaagatgcc cttaccctgc gccatctgca gcttctgcac
     4561 cagaaccggt gaatctcact caacaggctt catttcccct gacgccagcc tgaatacagc
     4621 tggcgttttc atttataaac agaaaggaaa accgataata atggaatgga cgccccgacc
     4681 atgaagcaac ggtaggatat tactgataaa ccttccagtg gaggtgcgtt atggccgttt
     4741 taacgattag cattgacctg acaaaaaatg aattccaaat ccacggtctg ggtaggaaca
     4801 gaaaaatcta agctcagaaa acggattaaa gtaagcgtca acggagcacc gtattgacgc
     4861 ttatttattg gtgagaacta cgttccatgg caggagttcg tcaacacggt tggagggcca
     4921 ttccggcagt atgctcagaa tatggcgcag atacgcttcc ggatcgatac cgttcagacg
     4981 gcaggtgcct gcttttccgg caacaagcag gaatctcagc gctgaagcag gaattagagg
     5041 taaaaacgcc ctaccgggcc atgaatcacc cggtcattgg agtagtaacc aaagcagatt
     5101 tagccagcat ggaacaaata tctttggtga aatgttggtt acgggaagct ggagcgcaca
     5161 acgtgttagt aaccagtgcg gttaataaca atggagttac agaacttttt gccttgctgc
     5221 atacagaaga aggctgccgc taatttcgat aggttctcag atgagaatga tgacggtgat
     5281 acacagataa gaatcaatga gcaatgaggg ttgccgggca accctcattg aataaaacag
     5341 gaattacttc gcttcgccgt ttttcatttc gcccatagct ttcagctttt tggagatatc
     5401 gcggcgttct ttggagagct cggcattttt gatgatgtaa tcgtcaacgc gatcttcata
     5461 gtcacctttc atgctggcga tgatgccctg gattgcttca acgctcatcc ctggcttgat
     5521 gtaatcgctc aggttgtcca gcaacaggac gcgcttctgg ttgtcacgga tctttttctc
     5581 aacgtcctga atttcacgtt gcagtttgtt cttacgacgg aacagacgaa caaattccag
     5641 tacgtcctgg aatgaaggct tggtagtttc catttttata cccctgataa tgtgagagtc
     5701 ggattcgttt aagcaaccgc tattcgttag ggccaacatt actcatagct gccgcagatg
     5761 acaatgcttt tatcccttat cactatcata ccctttatcc ttgctgaatc gaagcagcag
     5821 caagatgatt ctgaagttca ggaattattt gcgcaaggcc cggcaaatca gactcgatag
     5881 caactcaagc tgatgtgccg tttccatgtt cagccacaca taaccataga ttggtgtttc
     5941 cacaaccttc aggtcatggt ggttattcag caactgacgc agctcttcca cagcatcgtt
     6001 caatttttcc gtattggcaa aaaccggctg cggattacct tcgtacagcg catgaacaag
     6061 gctcagcagt atttgctgca tcatgtgctg ggtatcacga agtttttgcg cgttcaataa
     6121 cacgaaatgg ctggggcgcg tggcccagta tgcattgatt tgcaactcca gcatacaaac
     6181 cagattgcgg ttaatggtct ggataccttc atatatcgat tttggaatac gagtttcttt
     6241 gctggcgggc gcaatcagcc cacgcatttt cacggcatcg gtcaggagtt tttgtagatg
     6301 gctttccaga cgtgggcgtt cgagtaagtt cggtgagaat gcagattgat agacccgatt
     6361 atactcggtc agacttttcg ccagttgaat gcgccagtgg atgaacgccc gttgtggcca
     6421 gataccggta aacaacattg ccagtaaaga gccgaggatc acatcgccgc ttcgccataa
     6481 cgccgtatca atttcacctg tcggggaacc cacaacaatt gccagcgtca ccccaatcaa
     6541 taatccttga tacggtttct tgcccagcgc cagccaaccg caaagaaaca tcgccgccgc
     6601 gcaccagact aacatcagcg gtaacgaaat taactccagt tgcagagcga taagacctaa
     6661 aatcgacccc aacaccgtac cgccaatacg ttcgaaggca cgagggacaa cgttccccca
     6721 gaacgagatt ggccccataa tcaccaccat ggtgaccagc ggccaggtgc cttccgggat
     6781 agtaaacagg cggataatga gaaaagtgag caggaatgcc agcgcgaccc gagtaccatg
     6841 cacaatgcgg taatggcggt atacccggat ttcaaacggg cttaatgact tattggcacg
     6901 cacgcagaaa ctccgttaat gaagattaaa agcgctcata acacatctgt cggtcagaag
     6961 gcgcgcctct gaattatccg acggacaaac cgggaacagt gtaatttccc ggcaggagcg
     7021 aaaaaattga aaggcgcatc ttactctttt cgctttcaaa aaaagagtgg tcttcgcgtc
     7081 aacacaccgc cctgagatga attagtgatg ttttggctgc accgcaacat acatttcgat
     7141 atcccagtac ccatcttccg cgccattgtt caagtaaacc tcgaagcagg gctttggtaa
     7201 catttcataa gcactgtcct gcaagaggct attaaagaac tgataccagg gtttagcaaa
     7261 atcatcaccg actacacgag ccaccgccac cgcatattga ccacctgaaa tttctgtcag
     7321 aatgacgccc tcactgtttt cggggagcgt aaagttattc ggtaccgtca cgacggtgtc
     7381 gcagcgtaat ttttcggcgg gtgtttcatc cggattgtcg taatagacag caacccactc
     7441 cttcggcaca atatttttgc tatctaccca catcatcaac tgctcaaaac ctttctttac
     7501 cgtctgttcc cacgggccaa cgagatggaa acctgcaacg gtacgtttat cttcctgctt
     7561 aatctcgtag ttcatgacgc ctccattatt actgttttta tatacagtat agttgcaata
     7621 ttaaaaccac aaggaatgag tgttgattat gcgagcagac tcgcactcct gccagtctgc
     7681 tgcaaaagaa aggtcaggcc ttatggtgga aataatcact caggcgagaa aacatgctgc
     7741 cttccccgac agattccagg gtaaccagcg gccagtgcgc cacctgttta tcacggtcgt
     7801 aaagttcaat ttcccctacc cgctgatggg cgctaattgg cgcggtgagc tctttaccat
     7861 caagggtata tttggctttg atatgtggaa tttcggcttt cggtagcacc atccagaact
     7921 cttgttccgt tcccagggcg atattttctt tatcgccata ccagatgcgt tccgtaccga
     7981 cctttttccc acggtgcaaa atttgcaccg tagtaaagtt ttgttgcccc caacgcagta
     8041 attttcttgc ctcttcctca cgaccttttg cactgtcagc ccccattacc actgcaatga
     8101 gacgacgctg cccatctaca gccgaagcaa tgagattaaa cccggcacca gaagtatgac
     8161 ccgttttcag gccgtcaaca ttcatggttt tatcccacaa taacccgtta cggttttgct
     8221 gggtgatacc gttccaggtg agacttttct cactgtacat atgataaaac tcgggctcgc
     8281 cgtggatgat agcgcgagaa agcacagcta aatcataagc cgagctatgc tgacctggtg
     8341 catccagacc atgcactgtt tcaaaatgcg tatccttgag atgcagcttc tcggcatagt
     8401 tgttcatcat ttcaacaaac tgccgttgcc caccggcaat atagtcagcc agtgcaacac
     8461 aagcgtcatt tccggaatcc acaattaaac cacggcttaa atcacgtacc gatacgcgat
     8521 cgccctcttt caaaaacatc agtgaagaac cgacaaacac tggattatct ttcgcccacg
     8581 catcgcgccc cacggtgaca atatcgtctg gcgtaatgcg atgactatcg atagcgcgat
     8641 ccacgacata gcccgtcatc agctttgtca ggctggcggg attgcgctgt tgatgctcat
     8701 tacccgcggt gaggatctga ccggtggtgt aatccatcaa tacccaggag ccggcatgaa
     8761 tctctggagg ctgaggtgaa aaaggaatgt tttccgccgc aaaaccagac gataagttaa
     8821 aaatgaacaa agaagcagca ataataagac ggcgtttcaa cagcaaaccc tcaggagttt
     8881 caaatagctg ttctttttac ggaaatactt atgaactggc tggaataaag tgcaagaaaa
     8941 tgtgactacc ctctcatttt tatctgacat gatctgttgc cactcgctgt caaattgtcg
     9001 cgctaaagct gattagcacg gtgatatttg atactctggc agacagcaga aacaacggat
     9061 ttaacctaat gatgaatgac ggtaagcaac aatctacctt tttgtttcac gattacgcgc
     9121 aacacccctg agaaccgcca tgaacaagtg tttcatgatt tgtgatttta gtttggtacg
     9181 caatttggta cacaacacaa ttttcacttc agtgggtagt catcaaactc accataacga
     9241 gcatcgttga tgatgtatgt tataaccaca aacatcacat ctggactagt tccgttttca
     9301 tgagcgggta tttgtttacg cctccctttc tgcaaatcct aaagatgaga ttttggggtt
     9361 ttgggatatc ttttatcctc agttctctat ccattatgca aaccagtaat gaaccatcac
     9421 cgtgtgatag tgaggcgtct gccacgataa gcgcagagaa atgatgcaat ggtgggcgga
     9481 ctggcttgat gaaaaggtag agtgagcgac cttaaactat cgaatagaac aaagtcttgc
     9541 aatccagtgc aaagctttgt gcgcctcagt tttgtctaag cgttctactg aaaacatagt
     9601 aaaatcggta acggctggaa atcattcaat actcgcacta tcgaaagttc gccagccagc
     9661 cgtggcacgt tcttgcatac gacgtgccgc ggatcctatt attctagtat gaaatcccta
     9721 accgatttca aattctccag actgctcaga ttctctttta tcccacgcta gcgctgataa
     9781 atctcgtcat tgcaatcgac ctgcgctatc gctgccgcag ttcttccagt ttctgcatcg
     9841 ataact
//
LOCUS       DBFIFI010000161         9834 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.161, whole genome shotgun sequence.
ACCESSION   DBFIFI010000161 DBFIFI010000000
VERSION     DBFIFI010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.161"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..168)
                     /locus_tag="N7B39_003678"
     CDS             complement(<1..168)
                     /locus_tag="N7B39_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2423584.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWPCG
                     EFFSLNYKLTKR"
     gene            246..1340
                     /gene="ddlA"
                     /locus_tag="N7B39_003679"
     CDS             246..1340
                     /gene="ddlA"
                     /locus_tag="N7B39_003679"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HGU2423585.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1364..1576)
                     /gene="yaiZ"
                     /locus_tag="N7B39_003680"
     CDS             complement(1364..1576)
                     /gene="yaiZ"
                     /locus_tag="N7B39_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HGU2423586.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1836..2144
                     /gene="yaiY"
                     /locus_tag="N7B39_003681"
     CDS             1836..2144
                     /gene="yaiY"
                     /locus_tag="N7B39_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HGU2423587.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2203..3297)
                     /gene="yaiW"
                     /locus_tag="N7B39_003682"
     CDS             complement(2203..3297)
                     /gene="yaiW"
                     /locus_tag="N7B39_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HGU2423588.1"
                     /translation="MSRVNHLSSLSFLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSGIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAAKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3310..4530)
                     /gene="sbmA"
                     /locus_tag="N7B39_003683"
     CDS             complement(3310..4530)
                     /gene="sbmA"
                     /locus_tag="N7B39_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HGU2423589.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFALFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4882..6039
                     /gene="ampH"
                     /locus_tag="N7B39_003684"
     CDS             4882..6039
                     /gene="ampH"
                     /locus_tag="N7B39_003684"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HGU2423590.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6040..6663)
                     /gene="iprA"
                     /locus_tag="N7B39_003685"
     CDS             complement(6040..6663)
                     /gene="iprA"
                     /locus_tag="N7B39_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HGU2423591.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6751..9679)
                     /gene="ehaB"
                     /locus_tag="N7B39_003686"
                     /pseudo
     CDS             complement(6751..9679)
                     /gene="ehaB"
                     /locus_tag="N7B39_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaB"
BASE COUNT         2528 a         2439 c         2331 g         2536 t
ORIGIN      
        1 tctcttcgtt aacttataat taagagaaaa aaactcaccg catggccatt taagccagga
       61 ataaattagc ttcccgttca tcatttttgt gatcttgcgc acatctttga gataacgcac
      121 tttcttcacc agtacgttgc acgctttgtc agcagaagat tgcaacaatc tttcagaagg
      181 ccgcgaatcc cccgttttgt cgtggtaatg tatgcacttt gtgttagacg ggataggttt
      241 ttaagatgga aaaactgcgg gtaggaatcg tttttggtgg taaatcagcg gaacatgaag
      301 tgtctctgca atcggcaaaa aacattgtcg atgccattga taaaagtcgc ttcgacgttg
      361 tgctgctggg cattgataaa caagggcaat ggcacgtcag cgatgccagc aattatctgc
      421 taaatgcaga cgatcctgcc catattgcgt tgcgcccttc ggcgaccagc ctcgcccagg
      481 tgccaggtaa acatgagcat cagcttatcg acgcgcaaaa cggtcagccg ttgccgacgg
      541 tggatgtcat tttcccgatt gtccacggta cgctgggcga agatggttcc ttgcagggaa
      601 tgctgcgggt cgccaattta ccgtttgtag gttctgatgt tctggcttca gcagcctgta
      661 tggataaaga tgtcaccaaa cgtctgctac gcgatgccgg gctgaacatt gcgccattta
      721 ttaccctgac gcgcgctaat cgtcacaaca tcagttttgc cgaagtggag tctaaactgg
      781 ggttaccact gtttgtaaaa ccggctaatc agggctcttc tgttggtgtc agcaaagtaa
      841 ccagtgaaga acaatacgca attgccgtcg atctggcgtt cgagttcgat cataaagtga
      901 tcgttgagca agggatcaaa ggtcgtgaga tcgaatgcgc agttctgggc aacgacaatc
      961 cgcaagccag cacctgtggc gagatcgtac tcaccagcga tttctatgcc tacgacacca
     1021 agtacattga cgaagatggc gcgaaagtgg tagttccggc agccattgcg ccagaaatca
     1081 acgataagat ccgggcgatt gccgttcagg cttatcagac gttgggatgc gcaggcatgg
     1141 cgcgtgtaga cgtgttttta accccagaga acgaagtggt gatcaacgag atcaacacac
     1201 tgcctggctt caccaacatc agtatgtatc cgaagctgtg gcaagccagc ggtctgggtt
     1261 acaccgatct gatcacacgt ttgattgagc tggcgctgga gcgtcacgct gcggataacg
     1321 cattgaaaac cacaatgtaa tattttaacg cccgataata ttcttattgg gcgttttcag
     1381 tttcttcatc gcgacgacga ataatcaacc ccgccagcca gaaactaatc acccacgtca
     1441 ccagaccgac ggcataggtc tgccaacctt ttgcttcaaa tcccagtaac cccaccacgc
     1501 cattaagaat gaatataagg ccaatggcga acgcatagta gtgccagtca cggcggattt
     1561 ttacaggcag attcatggct gctccgttaa cgaaaaacaa catccttgca tattctgcaa
     1621 caaccgtctg tggtgtagac aacattttcg caattgttaa taaatttaac ttaaagttgc
     1681 tattttgtga ctcgcaacga agattcacgc ttcgggaata ttcccaacat caatttacca
     1741 tgattctgat attgacaatc taaatgacct gtcagacttg ccataagccg ctggtatcac
     1801 accagaagag aagcagatga cttccggagg tttttatggc tgatttcacc ctgtcaaaat
     1861 cgctgtttag cggaaaatat cgcaatgcct cttcaacgcc tggcaacatt gcctatgcgt
     1921 tgtttgtgct gttttgcttt tgggctgggg cgcaattgct gaacctgtta gtgcatgcgc
     1981 ccggcgtcta tgagcgttta atgcaggtcc aggaaacagg tcgcccacgg gtggaaattg
     2041 gtttaggtgt cggcaccatt ttcgggctga tcccgttttt agtaggctgc ctcatttttg
     2101 cagtggtggc gctatggctg cactggcgac atcgccgcca gtaagattta cgttgccaga
     2161 tacggcctaa aacgccttat ctggcatgac attcctgttt gttcactgtt tcatacaacg
     2221 cgcccgccgt tcgtctacgc gcttcgcaaa ccaggccgta gtcaggttgc gggtgatttt
     2281 cgggctttcc agttgaatgc caggcaacat ttcgcggggg agtgatttac ccgttttcgc
     2341 ttcggcaagt tgataaactt tcttgtacag cgccgtctcc tcaaaagaga agctgtcacc
     2401 tttctctagc tgacgacgaa tttcgctgtc gctcatcccc agttttgccg ccagtttgcg
     2461 cgttgccagt tcggttttcc cgggttcttt actgtcgtaa cgaattaaat caccgtccag
     2521 tgccagcttc acgccgctgg ccttactgac cgcgttctga aacgcggcat tacggctggc
     2581 gtaccagcca gcgttaaaat cagcaaaacg gtatatcggt gcgctataac tggcgggata
     2641 attcagtaaa tggtaagtac caaaccacaa cccgccgcgg cgactaaaga cttcctgacg
     2701 gactgtaccg tccattttcc acggataccc tttggtatgc tgttcggcaa aagcaatgct
     2761 gacctgcatc ggcccaccgg tgcgcaccgg attaagcgaa ccaaacaacg tctgccccat
     2821 tggcaccatg ctgatcagat cgtcaaaaat cccgctcaat tgcttttctg tacgcaccga
     2881 atctaaccgt tcgctataac ttttgccgtt tggcgatttg atttttagcg cagtatgaac
     2941 cagaaaagca ggaatgtgca tccgttcagc acgacggtca atctcttgcc aggcgatctt
     3001 gcttaagccc ggaacggccg gatctgcctg ataatttgac tcctgctgcg ccaccgccag
     3061 caccgagcag acgttttcca gcgtcggtgc cagcccctga ctttcaaagg tggtggcgag
     3121 atctttcgcc caggcatcgc ggtctttcac gctcgcaggc atcttctgcc gcaccacgct
     3181 agccacatct atcgcttttt cgcccttttt caacggctgt ggcgcctgac tgctacaccc
     3241 cgctaaaacc agaaccgcca aaaatgaaag cgaagacaga tgattgactc gcgacattac
     3301 ttctcctttt tagctcaagg tatgggttac ttcctgaatt ttgtcaccat ccagctcatg
     3361 ttcaaagctg cgcagacgtt tgtagataga catcaactca accagtgtgg tccatgagtt
     3421 aatcaggtac tggaaagcac cgcgaacctg accaaaaacg ttggtaatct gcgtcatcag
     3481 gccgagcgta atcgtaccgg caacaattga cggaaacagc aagaacaaac cgaaaacgtt
     3541 atcgacctgc aaatagagaa tgcgagcgat gttgaaatac atatagtgaa aatagaggcg
     3601 gaaatagttt ttccgtacgg cgctaaacag ctcgcgtacc gttggcggcg tcgcgcgcgt
     3661 ggcatcgtct tcaccataaa ccagctcttt acggtaggca gcctctacac gctggttttt
     3721 aaactccagc cccggcagtt tgatccctac cactgccagc aatccggtcc ccatcagcga
     3781 ccagacgata gcggcaatca ccagaccata cggaatgtgc ccgataatcg gcagctccgg
     3841 cacatgcgcg gagagcgtta ccaacaccgg caggaaggcg atcaacgtca tgatggcgtt
     3901 gataaaactg acacccatat tctccagcgt tgaagcaaaa cgcatggtgt cttcctgcac
     3961 acgctgtgcg gcgccttcga tatgacgcag ttgttgccag ttcgccatgt aatattcgtt
     4021 catcgccgta cgccagcgga acacgtagtg actgacaaag aagttattca gcacactgat
     4081 cactaccgcg atcagcgcaa tccccagaaa gacgcccact tcgcggtaaa attgttcgat
     4141 agtgacttta tgcggcgaac ttagcgcggt ttgaatcaga tcatagaacg gtgcatacca
     4201 ggcgttgacg gcaaccccaa cttccaccaa aaaccaggtg acgaagatga tcagtgcagt
     4261 accgagtatt gaccagtatt gccaacggtg cgggctgtag ataaaccaga acaatgcaaa
     4321 aagtcctacg caaacaatgt agtaagcgta aaaaatcagg aaatccaacg accagaaacg
     4381 cgcggcgcta atcgggatct gcccggaagc gccggtgata cgcgccaccc agtccccccc
     4441 accggcttgc cagaagataa cggcaatcaa tgcccaaaca aaggccgaga gaaaaaacgt
     4501 tcccggcttt gggaaaaaag acttaaacat atgcactcct gctaacttct tatcgtttta
     4561 acaactgctg tgtattaacc gcatgaccgc tacaaagcgt cagccaaatg aaaccgccca
     4621 ttgtgactca tagcaaggcg cttagttcgc cagcctggcc ataagaattg tttcgacgga
     4681 tttcacaaac caaagattgt ttacataagc ggattaacac gcgcaggatc acaaatgcag
     4741 gcattggtga gcaggaaaaa gatcgtgttt acgcgccgtt acgtttttaa cactattatt
     4801 gcactaattc gccctttgca atccatcaat gagtagtata aatacgctca gttaccttca
     4861 ttcaatttat ggacaccacc gttgaaacgt agtctgcttt tttctgccgt gctgtgtgcg
     4921 gcgtcattga cctccgtcca tgcggcgcag ccgatcactg aaccggagtt tgcctctgat
     4981 attgtcgatc gttatgccga tcatattttt tacggcagcg gtgccacggg gatggcgctg
     5041 gttgttatcg acggtaacca gcgcgtcttt cgcagttatg gcgaaacgcg acctggtaat
     5101 aacgttcgcc cgcagctgga ttcggtcgtg cgtattgctt ccctcaccaa gctaatgacc
     5161 agtgagatgc tggtgaaatt gctcgaccag gggaccgtga agttaaacga tccgctaagt
     5221 aaatatgctc cgccaggcgc acgcgtgcca acctacaatg ggacgcccat tacgctggtc
     5281 aatctggcaa cccataccag cgccctgccc cgtgaacagc ccggtggcgc ggcacatcgt
     5341 ccggtgtttg tctggccaac gcgcgagcaa cgctggaaat acctttctac ggcgaagctg
     5401 aaagccgcgc caggcagtca ggcggcgtac tctaaccttg cgttcgacct gctggcagat
     5461 gcactggcga atgcgtccgg caagccttat acccagttgt ttgaagagca gattacccgt
     5521 ccattgggga tgaaagacac cacctacact ccctcaccgg atcagtgccg ccgtttaatg
     5581 gttgctgagc gcggtgccag tccgtgcaat aacacgctgg cggcaattgg cagcggcggc
     5641 gtctattcca cgcctggcga tatgatgcgc tggatgcagc agtatttgtc atctgatttt
     5701 tatcagcgca gtaaccaggc agatcgcatg cagacgttga tttatcagcg cgcgcagttt
     5761 acgaaagtga ttggcatgga tgtccccggc aaagcggatg cgcttggttt aggctgggta
     5821 tatatggccc cgaaagaggg tcgtccgggg attattcaga agacaggtgg tggtggcggt
     5881 ttcattacct atatggcgat gatcccgcag aagaatattg gcgcgtttgt ggtggtcact
     5941 cgttcgccgt tgacgcgctt taaaaatatg agcgatggca tcaatgatct ggtgaccgag
     6001 ctaagcggga ataaaccgtt ggttatcccc gcatcctgat taatactctg aaggcaaacg
     6061 gttgatagcg accagtttgc ctttattcat ttcgatatag ccgcctttac gcaaagcagc
     6121 gagaacttct gcgacgacag aacgcgatat gcgtgtacgt tgatggatat aattcatcac
     6181 accaatacgt gatcgcaatt cttcattcca gtcaatcatt gataataatg ttgcgcggat
     6241 ttgttcgtag gaattatgcc caatgagctg cacgtcgcgt aattccagaa ttctattttg
     6301 ccaggctaac cagtaaaaag cgtctcgcca gagttgattt tgttcaataa gcgtaatggt
     6361 ttgtttggct ggtagatgat atcccgtaca atttccttct gatattaatt tgtatggtat
     6421 atcgtttttc attaaaccat cagccagccc cataatataa ggagcctggg taataccgat
     6481 aagtacgttt tcttctctac gcagagagat aactccctct aaaataacga aagtatcttc
     6541 gttattgaca tcactggaaa atataacttg cttttcatta ttaaactcga agcgcgtacc
     6601 gtatctggac aaacatttat cgagcttacc aaattcctga agaggtttaa ctacagataa
     6661 catttgcgcg tcctttgcag taatgcccgt caaatccttg acgggcatta tttagattaa
     6721 attaccagta tttcttcgga gtgaagaata ttaccaggta tatttaacac ccacgttcgc
     6781 ggaccagtct tgatctacgt caccaccacc gaggtagtta gcatcggtat aggcgctgaa
     6841 gttcttggtg aagctgaact gagtacccag accaacacgt accgcagacc cttcagtacc
     6901 gttatcgatg gaatcaccgt tcacatcgtt atcgttgtta gagtcgtcgt agacgtaagc
     6961 cagtttgaag tacggagtca gagcctgatc ttcgctgtag gtgaaggtat aacctgcatc
     7021 tacacccagt tcataacgca tgctgtcgta agactgaccg tcaactttca tgtcgttgct
     7081 cagctggtag tcatcaccag actggaacag accagaaatg ctgccgtaag gagtcacgta
     7141 accagcatca cccagtttga agtcgtaacc ggctttcaaa ccgaagcccc aagcgtcgga
     7201 gttggtgcta ccgtcaacgt aagtaccgtt gctcatggtt gcagacaggt cgttgttgaa
     7261 gtgagagtag ctcaagctac catcaacaaa gacgttgttc gcgaagtgag cagaagagta
     7321 gatgtaggca gtctggctgt cttgatccac ctgaccagaa cggtcattca tgtcaccttt
     7381 agcgaagcct gcagccgcac cgacgatcca cttagcgttg ttaccgtcaa ttttggtatc
     7441 aacaccgacc atgatgccgt taacatcctg atcatagttg atggtgccgt tgtcgccgtt
     7501 gaagttacca ccgaagtagc ttacccatgc gccgccgtta tcagccaggc catgacgaga
     7561 gttggtcaaa cgagtaccaa cggtgtcttg ttccaggttc cagatattgg tgttcgcaga
     7621 tgggatgctc agcgccatgt tagcgtagtc ggtcaactcc atctgttgca gaacaacggt
     7681 gttaccgcgc tgttcagcct gataggtgta tgcacccagg tcagctttgt tagcagcaga
     7741 gaaggtcgcg ttgctgtttt tgtcgttgac gtagatcagc tcgttgccgt tgtagtcagc
     7801 gatagaacca gcaccagtag cgttgtcgat acgaactttg tagttaccgg tcgcggctgc
     7861 aacatggtca ccgttgttat caacttcgtt ctgaccagct tcgatagaag cagtgttgtc
     7921 gttatcgccg ttaccgttaa tagtcaggtg accatcagag ttcattgcga ttaagccata
     7981 accgtagttt gacttggtag tatcgttggt acgatcgttg accagatcgg tatccattac
     8041 gtaatcactg ctatggatat cgaatacacc agcgtgaatg ttgccgttag tatcagtggt
     8101 actggtcaga ttcagggtat cggcctggaa cgggttagca tcgaactgat caacgttaat
     8161 atccaacaga ccgtcgttag ttacggtgat ggtatttgcg tacagtgcag catcttctaa
     8221 accccagccc gcagaaacat catcagacat tttaacggta ctattgtcga tggtcagggt
     8281 attagtcgct acatgaccgt cttcaccgat gttcaggtta gaaccgccag tcagagatca
     8341 gacgagagtc tgcaacatta acttgggaac cgctgttaat acttaaagta tcaatcagag
     8401 aagattttgt tgtatcccac tctgaaccac cgttcaaagt cacattaaac aaaccgctct
     8461 ggtaaacttc gtttcctacg atatggccat tttcgtcata ttgagtattg atgttatcaa
     8521 caccgacagt tgatgaaggc cacaggctgt tagctgcaat atcgagcaga gttgcagttg
     8581 cttcagtacc aacagcatat ccgtcgtaag aaccatcacc atcttcatca accatatgaa
     8641 cagacattgc agcgccaacc cacttgctgc cgttgttcag agtcacatcc atacggtctg
     8701 tgccatccca accgttggta tctacatcac catcagcatc gcgatagctg ttagcacctt
     8761 gcgggaagaa gttttcatcg aaattactgg agaaaacaac atcacccatc agtgttgagt
     8821 tgtctaaagt tactgtagtc tgcatcgcat tatcagcata cggatttgcg attgcggcaa
     8881 ttgcaacatc gtctgcagtc agcgtgttgc tatagttgct ggcattacca gtatggccaa
     8941 accaaccagt agtaccttca tcagtccatg aaccagaggt cacagtagaa tctttaacag
     9001 taatagtgtt attaaacact tcgctgctgt ttacgccagt gctgactgtg ttgtcgtcaa
     9061 tatcttccca ctcataaccc tgagtcagcg taatacctgc tacatgagag ttattagtga
     9121 tggacagatc aacttcctga tccagtgtaa tagcagtacc catatcgtaa acatctacaa
     9181 catgagtgtc agcggcatta ttataggtac cgttgtaagt ataatgctcg tagttgtcat
     9241 cgatcgttga attatcaacg ctcagtgtca gacgatcata aacataacca gtagcacggt
     9301 catcagcaca atcagtagtc atgcactcag aggtaatcat accgtgaata gtgctgtttt
     9361 tgattgtcag actattggcg ttggtattgg tagaaatacc gtcgtccaga tagtaggtag
     9421 aaatcacgcc gttaacggta gcgttattaa tgaccgggta gatatcgccg ttgtaatagc
     9481 tatcagcggc atagttgttc cagcctacat aaccatcata gtaaacgtca tcagcatagg
     9541 tggcgtcgtt gtagtgatgg aaagtattgt aagtttgacc agaaatatcg ttggtcgctg
     9601 cattagcctg agaggtgata gccagagtgc aagccaatgc taattgtgat actataagtt
     9661 tctttttcca ggagtgcatt tgtcatccct cctcagggac gtaataagtt gatccatcaa
     9721 ttatcaatgc atagaaaaca tcttatgttt cgcgttgacg attatgcggc gttatagagg
     9781 aatggttcaa ttattctttc ccctaagtcc gattgcggac atatataaaa atat
//
LOCUS       DBFIFI010000162         9781 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.162, whole genome shotgun sequence.
ACCESSION   DBFIFI010000162 DBFIFI010000000
VERSION     DBFIFI010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9781
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.162"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(209..475)
                     /locus_tag="N7B39_003687"
     CDS             complement(209..475)
                     /locus_tag="N7B39_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HGU2423592.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGQTEAIYCQQGSDWQCVVHYDAEASTRQ"
     gene            complement(472..1239)
                     /gene="yjiL"
                     /locus_tag="N7B39_003688"
     CDS             complement(472..1239)
                     /gene="yjiL"
                     /locus_tag="N7B39_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313324.1"
                     /note="YjiL, as found in Escherichia coli, is a homolog of
                     the activator ATPases of enzymes such as lactoyl-CoA
                     dehydratase, (R)-phenyllactate dehydratase, and
                     2-hydroxyisocaproyl-CoA dehydratase. The typical substrate
                     of those enzymes is acyl-CoA with an OH at the
                     beta-position. YjiL is the putative activator for the
                     cognate protein YjiM, a putative 2-hydroxyacyl-CoA
                     dehydratase with unknown specificity, encoded by the
                     adjacent gene; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-hydroxyacyl-CoA dehydratase activator
                     YjiL"
                     /protein_id="HGU2423593.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDCITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLESHLRMPVNTHPDAQFAGTIGAAVIGQRVRTRR"
     gene            complement(1249..2400)
                     /locus_tag="N7B39_003689"
     CDS             complement(1249..2400)
                     /locus_tag="N7B39_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HGU2423594.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKVRGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKAVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQFNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARIRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVVETGDVYDALADKYLAIGCSCVSPNDQRLKMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            complement(2516..3787)
                     /locus_tag="N7B39_003690"
     CDS             complement(2516..3787)
                     /locus_tag="N7B39_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HGU2423595.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQKKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMTARADAVKSYLKEDEAFLSELWGDLREWLKADINSEDSRVKERIARAGQWFGETLI
                     ADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKDLQF
                     IRVNGTLVGGCIGLILYLLSQLPALFPLGNF"
     gene            complement(3828..5060)
                     /gene="mdtM"
                     /locus_tag="N7B39_003691"
     CDS             complement(3828..5060)
                     /gene="mdtM"
                     /locus_tag="N7B39_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HGU2423596.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRKPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEEQ"
     gene            5527..6309
                     /locus_tag="N7B39_003692"
     CDS             5527..6309
                     /locus_tag="N7B39_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006685483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HGU2423597.1"
                     /translation="MTNFTTSTPHDALFKSFLMHPDTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRPLPLVIPMLFYHGSRSPYPWSLCWLDEFADPTTARKLYNAAFPLVDI
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVVLLVTECANDSQITALLNYIL
                     LTGDEERFNEFISELTSRMPQHRERIMTIAERIHNDDCRANS"
     gene            6284..6484
                     /locus_tag="N7B39_003693"
     CDS             6284..6484
                     /locus_tag="N7B39_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2423598.1"
                     /translation="MTIAERIHNDGWLLGREEGEQRILRLLLQNGADPEWIQKITGLS
                     AEQMQALRQPLPERERYSWLKS"
     gene            complement(6727..8139)
                     /locus_tag="N7B39_003694"
     CDS             complement(6727..8139)
                     /locus_tag="N7B39_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HGU2423599.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSISTVQ
                     QAYQTLETMKLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDLVLDMLVAHS
                     DSSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQVARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWVAPGRYHDKLM
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQIYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVTRQLCRMKIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYRAME"
     gene            8316..8480
                     /locus_tag="N7B39_003695"
     CDS             8316..8480
                     /locus_tag="N7B39_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HGU2423600.1"
                     /translation="MEFHENKAKTPFIRLVQLWQAVRRWRRQMQARRVLQQMSDERLR
                     DIGLRREDVE"
     gene            8577..9458
                     /locus_tag="N7B39_003696"
     CDS             8577..9458
                     /locus_tag="N7B39_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HGU2423601.1"
                     /translation="MKATEARLLDFLKRSQQFVIPIYQRTYSWTEQQCRQLWDDIIRA
                     GKRDDISAHFIGSVVYIEQGLYQVSGISPLLVIDGQQRLTTAMLLIEALSRHLGEDEV
                     FDGFSAMKLRNYYLLNPYESGEKGFKLLLTETDKDSLLALIKQRPMPENYSHRIMENF
                     TFFDEQIAKLGDDLIPLCRGLAKLLIVDVALNRGQDNPQLIFESMNSTGKALSQADLV
                     RNFILMGLEPEHQTRLYEDHWRPMEVACGQQGYSEYFDSFMRHYLTVKTGRSLGQMKS
                     MRHLNSMPAARVLLKKA"
     gene            9506..>9781
                     /locus_tag="N7B39_003697"
     CDS             9506..>9781
                     /locus_tag="N7B39_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2423602.1"
                     /translation="MALGKESDKSLATAFQDLRELKVDVAYPFLLALYHDYKNDDLSH
                     EDFLSIIRLIESYVFRRAVCAIPTNSLNKTFATFYKVINKEKYLESIQ"
BASE COUNT         2557 a         2478 c         2461 g         2285 t
ORIGIN      
        1 ccggtaatca ttttatttct ctatgcatga ttatgggttt aagtataatt caatgtatat
       61 tgtattttag acgcgcaata ggaaggtgct atcaaaaatc gctcattttg tggatgggaa
      121 atatttcacg gcaggcgaga tatttactac aacttccttc atgcaggatt atgcgcagcc
      181 aattatccgg ttggtatatt taaagatcct attgcctggt tgacgcttct gcatcgtaat
      241 gcaccacgca ctgccagtcg ctgccctgct ggcaatatat cgcttcggtt tgcccaggga
      301 tcacccattg tatttcctcg ccggggggac aggtcagcca aatgcataac ccgcagccgc
      361 cgcgtaaatc acggggaata tcgctgacgc gaaaagtcat gcccgctgcc tgcaacgcct
      421 tgcgggtttg tatgacgccg accgtggagt gaaataaaaa taaaaactct ttcatcggcg
      481 tgtcctcact cgttgaccaa ttaccgccgc gccaattgtg ccagcaaatt gcgcatcagg
      541 atgggtattt accggcattc gcaggtgaga ttccagcatc cgggcaaacg tctggcaatg
      601 actaacgcca ccagtaaaca gaatcggcgc ttcacaggag agacgagcaa tgaaattggc
      661 actccgccgc gccatcgcgt taatcactcc ggcgagaatc gcttctggcg cgacgcccgc
      721 tgagcgcagg ctgatcactt ctgattcagc aaacactgtg cacatactcg tgatggcgtg
      781 cggcgtgaca ttttcggtaa tgcagtcgag ttgctcgacg ctggtgccaa gcgtgcgcga
      841 gatcacctcc aggaaacgcc cggtgcccgc cgcgcatttg tcattcatca ggaaatcgca
      901 caggttaccg tcatcatcaa gctgaatcac tttgctgtcc tgaccaccga tgtcgattac
      961 cgcgcgcgtt gctggcgcaa gaaaccgtgc gcccagcccg tgacaggaga tttccgtcac
     1021 ttgtttatcg gcaaaatcca ccagttgccg cccgtagccg gtgagcgtca gaaacggcgt
     1081 tgtctctaac ccttcgcgca gagtttccca ggcttcagta attgctgttg ccggtcgaaa
     1141 gggtgttgga acgaggaaac ggcgcgtaat cacgccgtct gcgagtaaga tccctttggt
     1201 ggcggtagaa ccagaatcaa tgccaatcga atatgtcact gctactcctt acagcatctc
     1261 aataaaggcc gcgacacggg tactgagctg cccaacatcc gaggtggagt agtctgtttc
     1321 aatagcgata taaggaatgt tgtgctgctg acgcacatga cgtttaattg ccagcgattc
     1381 caccgcgtag gtatggcacg cctgcaaaat cacatcaact acgccatcga cctgatattc
     1441 ttccaccatc tggctgagca ttttcaggcg ctgatcgttc ggcgaaacac aggagcagcc
     1501 aatcgccaga tatttatccg ccagcgcgtc gtagacatcg cccgtttcta ccacgcattg
     1561 ctcggtcgct ttcgccccgg tgcagttttc ataaccgaca acccagccgc cattctcttc
     1621 aatcgcgcgc accactttct ctgctgcgcc gccaatcggg cagccggtga ttaaaatgcg
     1681 cggacgcggg tccagtcgct ggccttcttc ccactgctga cgaatgcggg cggtcatcgc
     1741 gtccagttca ttgatcaacg cctctttatc gaaccggaag gttgcgccgt aaaccacttt
     1801 cagaatgtcg ctgccgctaa gcgccggagg attgaactgc ccaagatgat aaaaattggc
     1861 cagtgcgcga cgttcgcggt ttttcagcgc aatggcatcg cgcagagcat cttcgctaat
     1921 ttcgtgccca aaacgttctt ccaccgcttt ttgtaagcgc agcatctcgg ctttccataa
     1981 cgcacgcgag gcatcgtctt taacgctgtt tggcagctgc atcacatgaa cgggcttaaa
     2041 ctccgccatg tattcataca ttttcttttt gccgtcgcag gtggtttcac cgaccaccag
     2101 atccgaaaag tagaagtagg ggcatttatc ggttttgccg aagccgtagc tacttttaat
     2161 cagcgggcag aggttgcgcg gcagatcttt ctccgcttct tcaatggttt catcagaggt
     2221 ggaacagagc gaaaccacaa ccgcaccggc tgccatcggg atctcttgcg gcataaaggt
     2281 gcagtaagtg ccaaccagcg gaatgccgcg caccttgaga tccatgacgg tgagaaagcc
     2341 tttctggcga gcttcagaga actgatcgaa aatggcgggt agatcggtga caagtgacat
     2401 gattttcctt ccccgtacca cgggagataa tgaaaagagg ccacattatt accattcttt
     2461 tagtatggtt tatttgatct ctctcgcggt tagccactta gtttttcatg gatttctaaa
     2521 aattgccgag ggggaacaag gccgggagct gtgacagcaa atataaaatt agcccaatac
     2581 aaccgccaac cagcgtaccg ttgacacgga taaactgcag atctttgccg atatttaact
     2641 ctatttgccg cgacatatcc cgggcatccc agctttttac cgtatcgctg atatggcgcg
     2701 tcaggaatgc agaaaactca ggcgcgacgc ggtgcgcggc ttgttccagg tgaccattta
     2761 acgacgcccg caaggcatca tcggcaatta acgtttcgcc aaaccattga cctgcccgcg
     2821 cgatacgttc tttcacacga gaatcttcac tgttgatatc cgctttcagc cattcccgta
     2881 aatcccccca caattcactg agaaaggctt catcctcttt cagatagctt tttacggcat
     2941 cagctcgcgc tgtcatttca ggatcgtttt tcaacttgtc gatcagggca aaagtggcgc
     3001 gatcaaatgc atggcggatc tgatgcgcac gatcgcggct gatatcatca agcaaagaat
     3061 tcaccgcgtc agaaaccaac tccgcgctat gttcgcccag ccattcggtg gggagaattt
     3121 tggctttcag tggatgctca ctctccagcc agcgaacaat ctgctgggcg ataaacttgc
     3181 gcgatttatc gcgctggaga agggcgatca actgtgcgat cagcgtatcc agcagcacct
     3241 gatgacgatc gtttttggtc atactctcca gcatcaacgc actggtgccg gaaagatcga
     3301 ccttatcaat cgcccgatgg accgcgcgct taagcaggcg ctgaatacgt gcatcatcgg
     3361 tcagttcaag aaaaccactc atgatctgca acagatgctg accaacgcgg cgggcgtttt
     3421 ctggctggct aaaccagttg ccaatcagca acgccggttc gtgacgtcga atcaatgcca
     3481 ccagcgattg ggtatcgaga aattttttct gcacgaactg accgagattt tcgccaatcc
     3541 ggtctttatt acgcgggata atcgccgtat gacgagaaat gatcggaatc ggcacgcggc
     3601 gaaacagcgc caccaccgca aaccagtccg ccagcgcgcc gaccatcgcc gcttcagcaa
     3661 tcgccttcac gccgctcacc caaaaattgg gcggcaaaaa cagcgtaacg acaaaggtag
     3721 cagcggcgat aagcagtaaa gagagcgcca acattttggc gcgtctgagt tcaatgagtt
     3781 tattcataac gttaagaata gagcctgacg gcaggatcgc gcaaaaatca ctgctcctct
     3841 gccagctcgg cagcctgatg ttggcgcacg cgattaagca atccggccag ggtgaaaacg
     3901 acgataacgc ccgccacaac ggctaacaga tgaaacggca agcgaccgcc gttaaaccac
     3961 agccagcggc caatttcgac cgagacagac atcaccatca ggatcaccat attcagcgat
     4021 gcggagacgg tccctttcgg taagttattg gaaaacagcg taaagcggaa taaggtcggg
     4081 aagatcaaac caatcccgaa agcatacaga ctggtgccca gcaccgacca cagccagacg
     4141 tgcggtgaca gcagattgcc gataatcaac agcgcgaggc cgaccagttg aatgggtacg
     4201 gcacgccaga taaagcgcgg ttcggtcgga tctttaacga aacgcgccac gatggcattc
     4261 gcgacaatca ccgcgccgaa caccggaacc tgtgtccagg cgaactgcga ggtcgttaag
     4321 ccgcctgcgt caatcaggat caccggcgag acagccaccc aactcatcat ggggatatag
     4381 cttaaagaga tggttgctgc gccaaagagg aacagccgat tgcaaaagac attacgaaaa
     4441 tcgcgcaaga cgcttttggc gctaaatgga accgcgccgc gcttcaccgt ctctggcatc
     4501 gccagcagta agccaacaaa tgagataaaa cccataaccg caatgatggc aaaaaggact
     4561 ttccagtgca caaagtgcat cagagctgcg ccggaaagcg ggccgataat cggcgcaatc
     4621 agtacgatgg aggtgataat cgccatcaac ttgatccctt ttgtctggcc gaacgcctcc
     4681 tgcaccgtga cataaccaac ggtggcaata aaacagatac tggtgccctg aattgcacgc
     4741 gcgataagaa actgtgtcat agacgttgtg aacattgtcg cagcgcaggc aagggtaaat
     4801 attagcgccc cggtaatcag cactggcttg cggccaatcc tgtcggaaag cggccccagc
     4861 agccactgca acgccatgcc tccagcgaga tagagactga cggcagccgg agccagactg
     4921 acatcggcat taaaatcacg gaccacatta atgatcccag gctggatcag gtccgtcgaa
     4981 agataggcgg caaagtcata caaaatcaac gccatcggga aaaacagcgt ggcggcatgg
     5041 cgggcaaaaa aacgtggcat aacaaacaac tccttgtccg gagaacccca acggggaaaa
     5101 cgaaattcaa taccgtcggt aatgacgaaa ggtaggcgag aaggacaaat gtactgaatt
     5161 tcatgtaatc aaccacactg cctgtgaaca cacagacaga gaaatacctt ctgacttcgg
     5221 gtcagaatgt ataacgatta caaatagtca tcagcacatt acacctcttg aattgatggg
     5281 ttgcatagaa aatatacacc ttaagtgtaa ttaaaatttg cgattaatca aaagagcgcg
     5341 gtggaagggg gaatatctgc cgggtacagg acatgtaaaa cgcgagtcag tttccggaaa
     5401 aatgtatcgt ttgcagacga acaaaaatag cctggaaagc gcctcgggaa acgagaaatt
     5461 gccgggtgag aatggttttg ttagtcgcta aagtcgggcc atcttatcaa caggtgacgg
     5521 atcgccatga caaacttcac gaccagcacg ccgcatgacg cattatttaa atcttttctc
     5581 atgcaccctg acaccgcgcg ggattttatg gagatccact tacccaaaga tttacgtgaa
     5641 ctgtgcgatc tcgacagctt aaaactggaa tccgccagct ttgtcgatga aaaattgcgg
     5701 gcgctacact ccgatattct gtggtcggta aagacccggg aaggcgatgg ctatatctat
     5761 gtggtgattg aacatcagag ccgtgaggat attcatatgg cctttcgcct gatgcgctat
     5821 tccatggcag tgatgcagcg ccatatagag catgataaac gccggccact accgctggtc
     5881 atcccgatgc tgttttatca cggtagccgt agtccttatc cctggtcttt gtgctggttg
     5941 gacgaatttg ccgacccgac tactgcacgg aagctttata acgccgcgtt cccgctggtg
     6001 gatatcactg tcgtgccgga cgacgagatt gtgcagcacc gcagagtcgc cctgttggag
     6061 ttgatccaaa agcatattcg ccagcgcgat ctgatggggc ttatcgatca actggtagta
     6121 ttactggtta cagagtgtgc taatgacagc cagataactg cgctgttaaa ttacatttta
     6181 ctgactggcg atgaagagcg ttttaatgag tttatcagtg aactaaccag tcgaatgcct
     6241 caacacagag agcgaataat gacgattgca gagcgaattc ataatgacga ttgcagagcg
     6301 aattcataat gatggatggc tgttgggaag ggaagaaggg gaacagcgca ttcttcgatt
     6361 gttgttgcag aatggggcag atcctgaatg gatacagaag attaccggac tttcggcaga
     6421 gcaaatgcag gcattaaggc agcccttgcc tgagcgtgag cgctattcat ggctcaagag
     6481 ctaaccagag acggatgaca aacgcaaagc agcctgatgt gctacgctta tcaggtctac
     6541 aaagcccatt caatatattg aactttaaag attttgtaga cctggtaagg cgttcacatg
     6601 gcatccggca tgaacaaagc gcactttacc taagccatgc cggaacgtgg ctatgatcct
     6661 gcaaagataa aacgagtcac aggcgattcg tgagaataaa tcaggtgctt cgccactaat
     6721 tctaatttat tccattgccc gatacacggc ctcgccaatt tgcttcagcg cttcgcgata
     6781 agtttcgctg agtggcaaag cacagttgat gcgcaggcag ttacggtatt tgccggaagc
     6841 tgagaaaatc gagcctgccg ccacctggat tttcatgcgg cacagctgcc gcgtgacgca
     6901 gaccatatcg acctgttcag gcaattctat ccacagtaaa aatccgcctt tcgggcgcgt
     6961 aatacagatt tcgcagggaa aatattcccg tatccagcag gtataaagcg ccaaattgcg
     7021 ctgatagatc tgccgcatcc gccggatatg gcgatgatag tgaccttcca gcacaaacgt
     7081 tgccgccgcc atttgcgtgg acggcacatt aaagctgctg atggcgtatt tcatatgcat
     7141 cagtttatcg tgataacgcc ccggtgcgac ccaacccacg cgcaggcctg gagcaatact
     7201 tttactgaac gagctgcaca acagcactcg cccgtcgata tcccaggaat gaatggtccg
     7261 cgggcgcgga tactccgttg ccagttcgcc gtagacatca tcttcaaaaa tcacaatatc
     7321 atgacgctga gcgagagaga gaacggcccg tttgcgcgcg tccggcataa taaatcccag
     7381 cggattatta cagtttggca ccagaatgat gcctttaatc ggccactgtt ccagcgccag
     7441 ttccagcgct tcaacgctga tgcctgtttc tggatcggtt gggatttcaa tcactttcac
     7501 gcccatgccg cgcagcatct gcattgaacc gtaataacag ggggattcga ccgcgacaat
     7561 atcgcccggt ttacacaccg ccattaacgc tagcgacatc gagttatggc agccgctggt
     7621 gatgatgatg tcatcggcag tgaccaccga gccgctgtcg agcatcaggc gggcaacctg
     7681 ctcgcgcaat acccgctgac cggctagcaa gtcataaccg agaacggttt gcagattatg
     7741 ctgcaccacc cggcttagct cccgccacag cggtttcagg ctgggcgttt cgacatccgg
     7801 cgtgcttttg cttaacggaa caatggaact gtcgctatgc gccaccagca tatccagcac
     7861 cagatcccac tgggtaattt ccactgggcg ctgcaccgga cgcgtcatcg gcggcactgg
     7921 cggctgggct ttacgttgtg cgacaaaata acccgaacgc ggctgcggag tgatgagctt
     7981 catcgtctcc agcgtctggt acgcctgctg cacggtgctg atgctgacgc cgtgctcctg
     8041 acttaagctg cgcaccgacg gcaatttctc cccgtgacga tacagccctt gctcaatccg
     8101 ctcggcaagt agagtcgcca gatgttgata acgcgtcatg ctgtattcct tatttggacc
     8161 atacagaaag aaaaaccggt acagatgagg ctaaaaaacc ggatgcagat gctgttttaa
     8221 cctgtctgta tgttaaacaa aactaatatt tgcatctgta ttgtgaactg ccagaagacg
     8281 gatgatgaag cctctgaaat atgaggaggc agcagatgga atttcacgaa aacaaagcca
     8341 aaacgccgtt tatcagactt gtgcaactct ggcaggcagt gaggcgttgg cggcggcaaa
     8401 tgcaggcccg acgggtgtta cagcagatga gtgatgaacg gttgagggat attgggttac
     8461 gcagggagga tgtggagtga ggggtatgtt cgtcgcggat aaacaggatt tctctataac
     8521 ctccataagg aagtggttat tattgcgaaa tcatcgtttc acggaaagac gtcagaatga
     8581 aagctacaga agctcgccta ctcgattttt taaaacgttc gcagcagttt gttattccca
     8641 tttatcaacg gacttattca tggacagaac aacaatgtcg gcaactttgg gacgacatca
     8701 ttcgtgcagg aaagcgtgac gatatatcag cgcattttat cggttcggtt gtttatatag
     8761 agcagggatt gtatcaggtt tctggtattt ctccgttgct ggtcattgat ggtcagcagc
     8821 ggctgacgac cgcaatgttg ctgattgagg ctttatcgcg ccatcttggc gaagacgaag
     8881 tttttgatgg cttttcggca atgaaattgc gtaattatta tttgctcaat ccttatgagt
     8941 ccggcgagaa aggttttaaa ttactgctga ccgagactga taaagacagt ttactggcgt
     9001 taataaaaca aagaccaatg ccagaaaact attcccatcg aataatggaa aactttactt
     9061 tctttgatga acaaattgcc aaactcggtg atgacttgat ccccttatgt cgtgggttag
     9121 caaagttatt aattgtcgat gtggcgctta atcgtggtca ggataatccg caactgattt
     9181 ttgaaagtat gaactccacc ggtaaggcgt taagtcaggc cgatctggtg cgcaatttta
     9241 ttctgatggg cctcgaacca gagcatcaaa cccggttgta tgaagatcac tggcgtccaa
     9301 tggaagtcgc ttgtggtcag caaggttaca gcgaatattt tgacagtttt atgcgtcatt
     9361 atctgacggt aaaaacgggg agatccctcg gacagatgaa gtctatgagg catttaaact
     9421 ccatgcccgc agccagagtg ttgctgaaaa aggcgtagat cggctggttg aagatattca
     9481 tatctacgcg gagtattact gtgcaatggc attgggaaaa gaaagtgaca aatcgcttgc
     9541 tacggctttt caggatttgc gcgagttaaa ggttgatgtg gcgtatcctt tcttactggc
     9601 gctttatcat gactataaaa atgacgattt gtctcacgaa gatttcctga gcataattcg
     9661 tttaattgaa tcttatgttt tccgccgtgc agtatgtgcg attccgacaa attctcttaa
     9721 caagacgttt gctacttttt ataaggtcat taataaagaa aaatatctgg aaagtattca
     9781 g
//
LOCUS       DBFIFI010000163         9763 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.163, whole genome shotgun sequence.
ACCESSION   DBFIFI010000163 DBFIFI010000000
VERSION     DBFIFI010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9763
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.163"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..773
                     /locus_tag="N7B39_003698"
     CDS             <1..773
                     /locus_tag="N7B39_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2423603.1"
                     /translation="GWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKLEKTLLIN
                     QLASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGF
                     TASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVLAIV
                     ALCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLLGAVGGFIAPILRVKAETLF
                     ASDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            799..1593
                     /gene="yfaU"
                     /locus_tag="N7B39_003699"
     CDS             799..1593
                     /gene="yfaU"
                     /locus_tag="N7B39_003699"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HGU2423604.1"
                     /translation="MINHPFKERLRKGEVQIGLWLSSTTAYMAEIAATSGYDWLLIDG
                     EHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKPLIKQVLDIGAQTLLIPMVDTADQA
                     RQVVSATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDNLDE
                     ILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAVAPD
                     MAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(1634..2560)
                     /gene="rpnE"
                     /locus_tag="N7B39_003700"
     CDS             complement(1634..2560)
                     /gene="rpnE"
                     /locus_tag="N7B39_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HGU2423605.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLEGLQEGMHEQAI
                     KIALRMLEQGFDRDLVLAATQLSEADLAANNH"
     gene            complement(2753..3943)
                     /gene="glpC"
                     /locus_tag="N7B39_003701"
     CDS             complement(2753..3943)
                     /gene="glpC"
                     /locus_tag="N7B39_003701"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HGU2423606.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSVAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(3940..5199)
                     /gene="glpB"
                     /locus_tag="N7B39_003702"
     CDS             complement(3940..5199)
                     /gene="glpB"
                     /locus_tag="N7B39_003702"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HGU2423607.1"
                     /translation="MRFDTIIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQAVTDIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELPHQRITPLGTLRSTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLAVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDQTLRPSQAGQTIENLFTIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(5189..6817)
                     /gene="glpA"
                     /locus_tag="N7B39_003703"
     CDS             complement(5189..6817)
                     /gene="glpA"
                     /locus_tag="N7B39_003703"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HGU2423608.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            7090..8437
                     /gene="glpT"
                     /locus_tag="N7B39_003704"
                     /pseudo
     CDS             7090..8437
                     /gene="glpT"
                     /locus_tag="N7B39_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311152.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
     gene            8442..9518
                     /gene="glpQ"
                     /locus_tag="N7B39_003705"
     CDS             8442..9518
                     /gene="glpQ"
                     /locus_tag="N7B39_003705"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HGU2423609.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
BASE COUNT         2240 a         2406 c         2692 g         2425 t
ORIGIN      
        1 ccggctggtt ctggatgttt gtgattgaag gattgttggc agtcggcgct ggggtattca
       61 cattcttttg gcttgatgac acaccggagc aggcacgttt tctgagtaaa ctagaaaaaa
      121 cgttgcttat caatcaactg gcaagtgaag aacaacagaa agtgacttct cggctgagcg
      181 atgcgctgcg taatgggcga gtctggcaac tggcgattat ctacctgacc attcaggtgg
      241 cggtttacgg attaattttc ttcctgccga cccaggttgc ggcattgctg ggaacaaaag
      301 tgggctttac agcgtcggtg gtcaccgcca ttccgtgggt tgcggccttg tttgggacct
      361 ggcttattcc gcgctattcc gataaaaccg gcgaacggcg taatgtcgca gcgctgacat
      421 tactggcggc gggcattggt attggtctgt ccgggctgct ttctccagta ctggcgatcg
      481 tagcgctgtg tgttgcagcc atcgggttta ttgccgtgca gccggtgttc tggacgatgc
      541 cgacacaact gctttccggt acggcgctgg ctgcggggat tggttttgta aacctgcttg
      601 gtgcagtggg cgggtttatt gccccgatcc tgcgcgtgaa agcagaaacg ttatttgcca
      661 gcgatgcggc gggattactg acgctggcag cggtggcggt catcggttcg ctgattattt
      721 tcactctgcg tgtaaatcgc accgttgcgc agaccgacgt ggcacatcat taaataggtt
      781 aaggaacacg atgaacgcat tattaaccat ccctttaaag aacgtttacg caagggcgaa
      841 gtgcaaattg gtctgtggtt aagctcaacg actgcctata tggcagaaat tgccgctact
      901 tccggttatg actggttgct gattgacggg gagcatgcgc caaacaccat tcaggatctt
      961 tatcaccagc tacaggcggt agccccctat gccagccaac ccgtgatccg tccggtggaa
     1021 ggcagtaaac cgctgattaa acaagtcctg gatattggcg cgcaaactct actgatcccg
     1081 atggtcgata ctgccgatca ggcacgtcag gtggtgtctg ccacgcgcta tcctccctac
     1141 ggtgaacgtg gtgttggggc cagtgtagca cgggctgcgc gctggggacg cattgagaat
     1201 tacatggcgc aagtcaacga ttcgctttgt ctgttagtgc aggtggaaag taaaacggca
     1261 ctggataacc tggacgagat cctcgacgtc gaagggattg atggcgtgtt tattggacct
     1321 gcggatcttt ctgcgtcgtt gggctacccg gataacgccg ggcacccgga agtgcagcga
     1381 attattgaaa ccagcattcg gcggatccgt gctgcgggta aagcggctgg ttttctggct
     1441 gtggctcctg atatggcgca gcaatgcctg gcgtggggag cgaactttgt cgctgttggc
     1501 gttgacacga tgctctacag cgatgccctg gatcaacgac tggcgatgtt taaatcaggc
     1561 aaaaatgggc cacgcataaa aggtagttat tgatatcaaa ggcccatggg gatcggctgt
     1621 gggactgtgt taattagtgg ttattcgctg ccagatcggc ttcgcttagc tgggtggccg
     1681 cgagcaccag gtcacgatca aagccctgtt ccagcatgcg caaggcaatt ttaatagctt
     1741 gttcatgcat gccttcctgc aaaccttcta atttaccttc ctgccaacca atttgatgtc
     1801 cttcctgccg tagccgttca gcaatagtca ttaatctctc cttttgtagt ggtgaacgtt
     1861 cggcaatctc ctgaataaaa cgggtgaaac gggaggtatc gccgcattgc agcagataat
     1921 taaacagtgt ttgtagctgg ctgtcattag tgaagcctct aaccaaaagc gtggtgatcc
     1981 tgtcgaccat accgattaaa tcgcggtcgc gaatatgctt ttgaatcagt tcaagcagag
     2041 ctatacgccg atgttgcatg atctcatcgt caggtacgat ggtaatatcc accaacggaa
     2101 aagcttcggt gtacaactgc cgggcaagtt gcggatcgtc aaactcatcc agccagttga
     2161 gtgagtacgg gtagggcgag gtttcgccat gataaaacag caacggcacc accagcggga
     2221 ctctgtcata gcctttgtcc aggtgacgct gcatggcggc agtggcatag cgcattagcc
     2281 gaaaagccat attcttttct gcagagcttt gatgttcaat cacgcagtag atatagccgt
     2341 caccgtcgct ggtttccacg gaccacaaaa catccgagta gtaagcgcgt aaactttttt
     2401 caataaaact agtgggttcc aggcgtaagg tttgcaggtt gcaaagcttg cgcagtggtt
     2461 ctggtaaatg tatttcgaga aaatcccgtg cggtttcggg tgtgaacata aaggttttaa
     2521 atactgcatc atgcggggag gaggttgttg attcggtcat ggttgtccgt cacctgatat
     2581 ttatgatgac gcgacattac ggttatccca ggcctttgac atctgccagt ttcagccttt
     2641 gcgagacgtt ttcagaggaa ttttcactgc gttgcaacgc atacattggg cacggaatcg
     2701 tgtacgcaaa aatgcccggt aaccacatgc ttaccgggca tcagaaagga gtttaagcca
     2761 gcgcctgggc cagtagcgta atcggatgtt cgcagcgaag acttgtggac atctcaatct
     2821 gccatttaca ggtttcgcag tcggtgacca ccagatctgc gccgctttct tctatctggc
     2881 ggaacagtgg tgcgccgatg gcttgtgagg tggggtagtt ctcttttttg aaaccgtaag
     2941 tacccgcaat accgcagcac tgggaatcca gcaccgttaa ctcaagcccc gggatgttac
     3001 gcaacagctc cagggtgtag agcgtccagc ccattttttc catatggcac ggagtgtgat
     3061 aaaccacttt cagcggcagc ggtttcagcg gtaacgtttt gccttcgtcc agcttgcgcc
     3121 acagccagcg ggtcgccagt tcgatatgat cgcgcaagcc tttgttgtcg acattcagca
     3181 cttccgggta ttcgtcgcgc agggcaaatg tacaggttga ggaggtggca atcaccggaa
     3241 tgccttttac tcccacagct tcgcggatcg actctacatt cgtaattgcc tgtttgcgtg
     3301 ctttatcggt aaagccgttg gcgatcagcg gtacgccgca gcatttttct ttgctgagca
     3361 gttgtacacc ggtacccatt gcgttgagca ctttaattaa atctttacct aactgcggat
     3421 ggttgtagtt aacgaagcag ccgtgaaaga aagcgacctg gtctttatac tgcgcttgct
     3481 gagccgccac gctgcgatac cagcgacgaa acgtgccgaa ggagtatttc ggtagcgtgc
     3541 ggcgatgatc gatttttaac gccgcatcaa gcaactgccg caccggtttc agcgaggtag
     3601 cggtgttgac gattggtgcg aacggcgtgg aaacgctacc catcaggtcg gtatggctta
     3661 aaacaaaatt gcgcagcgac gggcgcgtgg tgtcatattt cgcccgtgcg cgctggatga
     3721 tatcgccaat cttcacatcg gacgggcagg cgacttcgca acgtttgcag ttgatgcaat
     3781 atttcagtgc ctcgtcatac agtgcgccat cttttaaacg cagacgctcg ccatccggcc
     3841 cggcttgttt tggccctgga taaccgggat tcacccggct cactgggcag gcggtggtgc
     3901 agacggtgca cttaatgcag ttttcgaagc tggtgtcatt cattgttggc ctcctgcgcg
     3961 ttgggcaatc tgttgagcgg catgtaaagc actgacggca caaacaccgc cgccgcatcc
     4021 ctgggcgatg ggatcaaatc cgcccagcac cgaaccgatg gtaaacaggt tttcgatggt
     4081 ttgccctgcc tgcgacgggc gtagcgtctg atcagtggtt acaccgaact gctgccacgg
     4141 ttgcggcgca aaaaaatctc ccttatacca ttcaccccgc gtggcggttt gtagcacatc
     4201 aaggccgaga atcggctctc gaatgccgtt acgttccgct accagtccgc cactaaagaa
     4261 actgccgctg gcgagaaccg cgaaacgtgg acgcagcggg atatcggcgt gattgcgggt
     4321 ccagatttca ttcactacgc catttttaca ggtcactttt ttcacttcat cgcccggcat
     4381 ccacacgcca ccctgacgca caaactggcg ctgtaactgg ttttgcagac gaatgcccag
     4441 cacggaaggc ggcagcgttg gcaaaagcat cagtgaacaa ggtagttttt cattcaacca
     4501 acgccacagt ttgtcatcag ccaggccgaa gcaggcgggc atcaggatca tttcgcaggt
     4561 attggcgaca ggaataagcg catcaagtaa cagcggccag ttttcttcat tatcaaggaa
     4621 acgggcgata ttcaccgcgc ggaactcggt ggcgttattg cgcaggacat ccagttccgg
     4681 cagctctatt tctgcggttt caacggcaag gtcgagttca cgcaacgaag ctgccgcaag
     4741 gtgcgcctga aaatccatca gtccgctaat tcccactaca catattttct tcgcgggcag
     4801 cggccagacg gggacttctg gcgaacttag ccaggtagag cgcagagtgc cgagcggcgt
     4861 aatccgctgg tgaggcagtt ctacgctgcc ctgcaattgc gcaccgcttt cagcgattaa
     4921 tgcctgcgcc tggcaagcga gatcgagcac gcgttgtggc ccgagaagag agtaaggatg
     4981 tgcaggtgcc tgctgacgca aagattccag tccactgtga atgtctgtca cagcttgacc
     5041 atctggcaga tggctcagca aatccagcga accggatgag aaatgcagcg cgctttgacc
     5101 acgcgtgaca atggcacagc gcaggccgtg tttttgcagc tgcaggccac agagtaatcc
     5161 ggcgaggccg ccgcccataa tgatagtatc aaagcgcatc tttctgctcc ttctccagac
     5221 cacacagtcc ctgataaacc cagcgggtaa attcgctttc gcgcagtgca tctccccagg
     5281 cgatgggttg cacgcctttc cagcgttcgt taaggaaggt ggaaagttgc tcgatagatt
     5341 gcgcggacgt cgtgacgtta aaacgttgca gcagtccagc agcgcggcag gcgcagagtt
     5401 cgccctggca ggtacccatc cccacacggg tacgacggcg taaatccagc aggctattaa
     5461 cgtttaaatt ttctaccgcg tactgcactt cacccgcagt taccgcttcg cactcacata
     5521 ccaggctacg gtgcagacgg ccttcgctca gccaggcagg cgtgcgatcg ccatgacgat
     5581 aaaccgcaga accgcgcagc ggggcaggca gggagatgac tttacgcaag gtaacttcag
     5641 cgggttcctg tgaacccgga agtgccagat cggcggtcgt acaggggcgc gtgttgccca
     5701 gtttgcggca taccgcgtcg gtagcccatt cagccatcag ccgataggtc atcagtttgc
     5761 caccggtgat ggtgataaat ccgtccagac catcgcgttc agcatggtcg agcagcacga
     5821 tgccacggct gacgttacgt ccgctcgggt cgtcatcgct ggcaaccagc gggcgcacgc
     5881 cagaataggc ccgcaaaatg cgcgttttcg ccatcacggg ggccagtttt tccccttcac
     5941 gcagcagaat atcaacctct tctgccgtca ctcgattatc gtcaatctcg ttgtaatcaa
     6001 tacgtaaaga ggtggtgcca atcagcgaaa tggtatcgcc aggcaccaga atatcggcgt
     6061 cggaaggttt acggcagcgg ttgatcacat gctgattaat gcggtgatcc atgatcagca
     6121 gcgatccttt cgccgggaac atgcgaatgc gcagatcggc atattcggca atgtgttgcc
     6181 cccagatccc agcggcatta accacgacag gtgcatgaag ggcctgagtt tcgccggtga
     6241 gatggttacg tacacgaaca ccgcacaccg tcgcgccttc acgaatcagc cccgtgactt
     6301 catgagcggt aaggataaca gcaccgtgtt ctttggcatc cagcatgttt gctgcggtca
     6361 gacgaaatgg atcaacggtg ccatccggaa ctttcaccgc gccaatcagt gccgggttaa
     6421 cggcaggttc gataatgcgc gcttgctgcg ggtctatagc ttctgcgctg atccctgctt
     6481 cttcgcaggc gcgaataaaa gtggcctgga aggagaggtc atcttccggc agggtgataa
     6541 acaggccgtt ggttggttca acgcagtgac gtgcaatgcg tttcaggatc tggttttcac
     6601 taatgcattc gcgggccgat tccgcatcgg ttaccgcata gcgcgcaccg ctgtgcagca
     6661 ggccgtggtt acgcccggtg gcaccggttg cgatgtcgtg gcgctcaacc aaaatcacgc
     6721 gcagcccgcg cagggcacag tcgcgggcaa ttccggctcc cgttgcgccg ccgccaatga
     6781 taatcacgtc acttgattgc gagtcgcgag ttttcattgt tatccctctg aagttcgttt
     6841 tttaccattt agccatagta aaaacatgag ttgtttgatt tcgcgcatat tcgctcataa
     6901 ttcgaaagtg agacgtgatt tcatgcgtca ttttgaacat tttgtaaatc ttatttaata
     6961 atgtgtgcgg caattcacat ttaatttatg aatgttttct taacatcgcg gcaactcaag
     7021 aaacggcagg ttctctcact gaatcaggct gttaatcata aataagacca cgggccacgg
     7081 aggctttcaa tgttgagtat ttttaaacca gcgccacaca aagcgcgctt acctgccgcg
     7141 gagatcgatc cgacttatcg tcgattgcgc tggcaaattt tcctggggat attctttggc
     7201 tatgctgctt actatttggt tcgtaagaac tttgcgctcg ctatgcctta cctggtggag
     7261 cagggattct cacgcggtga tttaggtttt gcgctttctg ggatctcgat cgcctatgga
     7321 ttttcgaaat tcatcatggg ttcggtatcg gatcgctcga atccgcgcgt tttcctgccc
     7381 gcaggtttga ttctggcggc ggcagtgatg ttgtttatgg gctttgtgcc atgggcgacg
     7441 tcgagcattg cggtgatgtt tgtactgttg ttcctctgcg gttggttcca ggggatgggg
     7501 tggccgccat gcggtcgtac tatggtgcac tggtggtcgc agaaagaacg tggcggcatt
     7561 gtgtcagtgt ggaactgtgc gcacaacgtc ggtggtggta ttccgccgcc tggggatggc
     7621 ctggttcaat gactggcacg cggcgctcta tatgcccgct ttctgcgcca ttctggtggc
     7681 attattcgcc tttgcgatga tgcgcgatac cccgcaatcc tgtggcttgc cgccgatcga
     7741 agagtacaaa aatgattatc cggacgacta taacgaaaaa gcggaacagg agctgaccgc
     7801 gaagcaaatc ttcatgcagt acgtgctgcc gaacaaactg ttgtggtata tcgccatcgc
     7861 caacgtgttc gtttatctgc tgcgttacgg catcctcgac tggtcaccga cttatctgaa
     7921 agaggttaag catttcgcgc tggataaatc ctcctgggcc tacttcttct atgagtatgc
     7981 gggtattccg ggcaccctgc tgtgcggctg gatgtcggat aaagtcttcc gtggcaaccg
     8041 tggggcaacc ggcgttttct ttatgacact ggtgaccatc gcgactatcg tttactggat
     8101 gaacccggca ggtaacccaa ccgtcgatat gatttgtatg attgttatcg gcttcctgat
     8161 ctacggccct gtgatgctga tcggtctgca tgcgctggaa ctggcaccga aaaaagcggc
     8221 aggaacggca gcgggcttta ccggtctgtt tggttacctg ggcggttcgg tggcggcgag
     8281 cgcgattgtt ggctacaccg tggacttctt cggctgggat ggcggcttta tggtaatgat
     8341 tggcggcagc attctagcgg ttatcttgtt gattgttgtg atgattggcg aaaaacgtcg
     8401 ccatcaggaa ttactgcaaa aacgcaacgg aggctaatgg catgaaattg acgctgaaaa
     8461 accttagcat ggcgatcatg atgagcacta tagtcatggg aagcagtgca atggcggcgg
     8521 acagcaacga aaaaatagtc atcgcccatc gcggtgccag tggatatttg ccggagcata
     8581 cgctgccagc aaaagcgatg gcgtatgcgc agggagcgga ttatctggaa caggatttgg
     8641 tgatgaccaa agacgaccat ctggttgttc tgcatgacca ttacctcgat cgtgttactg
     8701 atgttgccga tcgtttcccg gatcgggcgc gcaaagacgg tcgttactac gcgatagatt
     8761 tcacgctgga tgaaattaag tcgttgaaat ttaccgaagg tttcgatatt gaaaacggta
     8821 aaaaagtgca gacttatcca gggcgtttcc caatgggtaa gtccgacttc cgggtgcaca
     8881 cctttgaaga agagattgaa tttgttcagg ggttaaatca ctctaccggg aaaaatatcg
     8941 gtatctatcc agaaatcaaa gcgccgtggt tccatcatca ggaagggaag gatattgcgg
     9001 caaaaacgct ggaagtgctg aagaaatatg gttacaccgg taaagacgat aaagtttatt
     9061 tgcaatgttt tgatgctgat gagctgaagc gtattaagaa tgagctggaa cccaaaatgg
     9121 gcatggatct caatctggta cagctgattg cctataccga ctggaatgaa acgcagcaga
     9181 aacagccgga cggaagctgg gttaattaca actacgactg gatgtttaag ccgggtgcca
     9241 tgaaacaggt ggcggaatat gcagatggta ttggtccgga ttaccatatg ttgattgagg
     9301 agacatcgca gccgggtaat atcaaactca ctggcatggt gcaagatgct cagcagaaca
     9361 agctggtagt gcatccttat accgtgcggt cagataaact gcctgaatac acaactgatg
     9421 tgaatcagtt atatgatgct ctgtataaca aagcgggtgt aaatgggttg tttactgatt
     9481 tccctgataa agcggttaaa ttccttaata aagagtaatg ctgctactgc aggatggcgt
     9541 tcatgcgcca tcctctgcta aagcgaaata atgctactac cggttattct tttcattaac
     9601 cggtgcgtcc tgtaaaaaag atgccaacat aattttcgta aaactcattc gttaaaaaag
     9661 ctaacactgg gcattgttat tctttaaaca gtatgaatag gtctcaataa taaattgtaa
     9721 tgcagtttat actgtaaagt attattctaa tttcggaaag gta
//
LOCUS       DBFIFI010000164         9757 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.164, whole genome shotgun sequence.
ACCESSION   DBFIFI010000164 DBFIFI010000000
VERSION     DBFIFI010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9757
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.164"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            1..696
                     /gene="yneK"
                     /locus_tag="N7B39_003706"
                     /pseudo
     CDS             1..696
                     /gene="yneK"
                     /locus_tag="N7B39_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
     gene            847..2037
                     /gene="ydeA"
                     /locus_tag="N7B39_003707"
     CDS             847..2037
                     /gene="ydeA"
                     /locus_tag="N7B39_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HGU2423610.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHLSMSMIGYVGTVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(2062..2727)
                     /gene="marC"
                     /locus_tag="N7B39_003708"
     CDS             complement(2062..2727)
                     /gene="marC"
                     /locus_tag="N7B39_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HGU2423611.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNGAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TNH"
     gene            2939..3373
                     /gene="marR"
                     /locus_tag="N7B39_003709"
     CDS             2939..3373
                     /gene="marR"
                     /locus_tag="N7B39_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HGU2423612.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            3393..3776
                     /gene="marA"
                     /locus_tag="N7B39_003710"
     CDS             3393..3776
                     /gene="marA"
                     /locus_tag="N7B39_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HGU2423613.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            3808..4026
                     /gene="marB"
                     /locus_tag="N7B39_003711"
     CDS             3808..4026
                     /gene="marB"
                     /locus_tag="N7B39_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HGU2423614.1"
                     /translation="MKPLLSAIAAALILFSAQGVAEQTQQPLVTSCGDVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQQAM"
     gene            complement(4057..4956)
                     /gene="eamA"
                     /locus_tag="N7B39_003712"
     CDS             complement(4057..4956)
                     /gene="eamA"
                     /locus_tag="N7B39_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HGU2423615.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAISGVLVLIEDSFNGQHVAMLGFMLTLAAAFSWASGNI
                     FNKKIMSHSSRPAVMSLVVWSALIPIIPFFVASLILDGSGKMIHSLVTIDMTTILALM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMAGLYINVFGLQWRKAVKVRG"
     gene            5151..6338
                     /gene="ydeE"
                     /locus_tag="N7B39_003713"
     CDS             5151..6338
                     /gene="ydeE"
                     /locus_tag="N7B39_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HGU2423616.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTVGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMFFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLIGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAVNPLVSGVVLTTLPSSSLFIILALVIIAAWALMLKGINARPQRQP
                     ALC"
     gene            complement(6379..6774)
                     /locus_tag="N7B39_003714"
     CDS             complement(6379..6774)
                     /locus_tag="N7B39_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HGU2423617.1"
                     /translation="MRYVTEFTTAKPEVAEAHFLARLSVETDCADVHDSLAYIGDDFV
                     LLHVVGREEDFARRHIPGALHLPHKQMTAERMAEWPDDTLFVVYCAGPHCNGADRAAL
                     KLARLGKPVKIMLGGITGWEDEGYAFASE"
     gene            6869..7840
                     /gene="ftrA"
                     /locus_tag="N7B39_003715"
     CDS             6869..7840
                     /gene="ftrA"
                     /locus_tag="N7B39_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FtrA"
                     /protein_id="HGU2423618.1"
                     /translation="MTTLQRPLVIALAYDGLCTFEFGVAVEIFGLPRPELGDNWYRFA
                     VAAVEPGALQATGGIHVVADGGLELLPNADMIIIPGWRGIDAKVPEMLCQSLREAHQR
                     GTRLVSICSGVFVLAAAGLLHQRKATTHWRYTEQLQHRYPSISVSDNVLYQDEGQVMT
                     SAGSAAGIDLCLHIVRKDFGHEIANNVARRLVIQPHRQGDQPQNLTRPMASPRESQTL
                     GALFDFLQQNLAQTHTVSSLAERVNMSPRTFLRRFEALTGMTPARWLLNERLIRVQDY
                     LQNSVFNIEKIAELTGFGNSALLRHHFHQRFLLSPSQYRKNSRSPSQ"
     gene            7948..8043
                     /gene="mgtS"
                     /locus_tag="N7B39_003716"
     CDS             7948..8043
                     /gene="mgtS"
                     /locus_tag="N7B39_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HGU2423619.1"
                     /translation="MLGNMNVFMAILGIILFSGFLAAYFSHKWDD"
     gene            8043..8147
                     /gene="mgtT"
                     /locus_tag="N7B39_003717"
     CDS             8043..8147
                     /gene="mgtT"
                     /locus_tag="N7B39_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HGU2423620.1"
                     /translation="MNGDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(8262..9152)
                     /gene="dgcZ"
                     /locus_tag="N7B39_003718"
     CDS             complement(8262..9152)
                     /gene="dgcZ"
                     /locus_tag="N7B39_003718"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HGU2423621.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLRPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGVLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEETLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(9407..>9757)
                     /gene="ydeI"
                     /locus_tag="N7B39_003719"
     CDS             complement(9407..>9757)
                     /gene="ydeI"
                     /locus_tag="N7B39_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HGU2423622.1"
                     /translation="PYALADDQGGLKQDAAPPPPHAIEDGYRGTDDAKKMTVDFAKTM
                     HDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPATVFDGREVQPDQMINISGSLDK
                     KSAPAVVRVTHLQK"
BASE COUNT         2524 a         2437 c         2193 g         2603 t
ORIGIN      
        1 tttcagaccg gcgtcgagcg tgtgctcttt ctgtttctga atgattttat tgaacaattt
       61 ccaatgatca accctggcgt tcccatcaaa agagcgcata caccacatat tgaacccctg
      121 cctccggatc accataccgc tgcagattac ttacgccagt ttgatttgct tgtcctgaat
      181 tttatctctc gcggtaattt tgtcatactc ccccgattat ggaataactc tgaggttcac
      241 agatggttcg tcaataaaga tcctaacttg atcaccgcca ttctcgacat aacggacagc
      301 gaattaaaag aggatttatt gcaaagcctg atggattcat taggttctaa caaacatgta
      361 ctaccctaag tctgcatctg ttttttatcc cttttagcag aacaagaatc tcctcatttt
      421 caggacttgt ttttattttt cgccaatatg ttgttacact atcaccaatt tatgaatccc
      481 aatgaaagtg atttgaatga cgtgttgatg ccagcatcat taagtgatga taaaattatc
      541 aaacatatgg cgcgtaagac cctcaaactg tttgtaaaaa atgaaacacc gccaaaagtt
      601 actcatgaag atctggtgaa aaacaggcct cgctcccctg tcagaccgcc tatacccgca
      661 acctccaaaa cgccagacat tcctgatcgt cattaaaccg tgatgtttac catctctctg
      721 acgcgtgaaa gaatcagcgt cagagaaacg gaaaacgcga tccagatcac aaatgcattg
      781 tattcacatc attaaccgtt ttaagatcat ttcatcactt tttcgcaact cacccgataa
      841 tctgttatga caacaaacac tgtttcccgc aaagtggcgt ggctacgggt cgttacgctg
      901 gcagtcgccg ccttcatctt caacaccacc gaatttgtcc ctgttggcct gctctctgac
      961 attgcgcaaa gttttcacat gcaaaccgct caggtcggca tcatgttgac catttacgca
     1021 tgggtagtag cgctaatgtc attgcctttt atgttaatga ccagccaggt tgaacggcgc
     1081 aaattactga tctgcctgtt tgtggtgttt attgccagcc acgtactgtc gtttttatcg
     1141 tggagcttta ccgttctggt gatcagtcgc attggtgtgg cttttgcaca tgcgattttc
     1201 tggtcgatta cggcgtccct ggcgatccgt atggctccgg ccgggaagcg agcacaggca
     1261 ttgagtttaa ttgccacagg tacagcactg gcaatggtat taggattacc tctcgggcgc
     1321 attgtggggc agtatttcgg ttggcgaatg accttcttcg cgattggtat cggggcgctt
     1381 attacccttt tgtgcctgat taagttactt cccttactgc ccagtgagca ttccggttca
     1441 ttgaaaagcc tcccgctatt attccgccgc ccggcattga tgagcattta tttgttaact
     1501 gtggtggttg tcaccgccca ttacacggca tacagctata ttgagccttt tgtgcaaaac
     1561 attgcgggat tcagcgccaa ctttgccacg gcattactgt tattactcgg tggtgcgggc
     1621 attattggca gcgtgatttt cggtaaactg ggtaatcaat atgcgtctgc gttggtaagt
     1681 acggcgattg cgctgttgct ggtgtgcctg gcactgctgc tacctgcggc gaacagtgaa
     1741 atacacctcg gggtgctgag tattttctgg gggatcgcga tgatgatcat cgggcttggt
     1801 atgcaggtta aagtgctggc gctggcacca gatgccaccg acgtcgcgat ggcgctattc
     1861 tccgggatat ttaatattgg aatcggggcg ggtgcgttgg taggtaatca ggtgagtctg
     1921 cacttgtcaa tgtcgatgat tggttatgtg ggcacggtgc ctgcttttgc cgcgttaata
     1981 tggtcaatca ttatatttcg ccgctggcca gtgacactcg aagaacagac gcaatagttg
     2041 aaaggcccat tcgggccttt tttaatgatt cgttttaatg atttccagga tgccgttaat
     2101 aataaactgt acacccatac ataccagcag gaaccccatc aagcgggaga tcgcttcaat
     2161 gccacccttg cccaccagcc gcataattgc gccagagctg cgtagacttc cccacaaaat
     2221 aaccgccacc aggaaaaaga tcagcggcgg cgcaaccatc agtacccaat cagcgaaggt
     2281 tgaactctga cgcactgtgg acgccgagct aataatcatc gcaatggttc ccggaccggc
     2341 ggtacttggc attgccagtg gtacaaaagc aatattggcg ctgggttcat cttccagctc
     2401 ttccgacttg cttttcgcct ccggtgaatc aatcgctttc tgttgcggaa aaagcatccg
     2461 aaaaccgata aacgcgacga ttaagccgcc tgcaattcgc agaccgggaa tcgaaatgcc
     2521 aaatgtatcc atcaccagtt gcccggcgta atacgccacc atcatgatgg caaagacgta
     2581 caccgaggcc atcaacgact gacgattacg ttcggcaccg ttcatgttgc ctgccaggcc
     2641 aagaaataac gcgacagttg ttaatgggtt agctaacggc agtaacacca ccagccccag
     2701 gccaattgct ttaaacaaat ctaacattgg tggttgttat cctgtgtatc tgggttatca
     2761 gcgaaaagta taaggggtaa acaaggataa agtgtcactc tttagctaac cttgcatcgc
     2821 attgaccaaa acttgaaccg atttagcaaa acgtggcatc ggtcaattca ttcatttgac
     2881 ttatacttgc ctgggcaata ttatcccctg caactaatta cttgccaggg caactaatgt
     2941 gaaaagtacc agcgatctgt tcaatgaaat tattccattg ggtcgcttaa tccatatggt
     3001 taatcagaag aaagatcgtc tgcttaacga gtatctgtct ccgctggata ttaccgcggc
     3061 acagtttaag gtgctctgct ctatccgctg cgcggcgtgt attactccgg ttgaactgaa
     3121 aaaagtgttg tcggtcgacc tgggagcact gacccgtatg ctggatcgcc tggtctgtaa
     3181 aggctgggta gaaaggttgc cgaacccgaa tgataagcgc ggcgtactgg taaaacttac
     3241 caccagcggc gcggcaatat gtgaacaatg ccatcaatta gttggccagg acctgcatca
     3301 agaattaaca aaaaacctga cggcggacga agtggcaaca cttgagcatt tgcttaagaa
     3361 agtcctgccg taaacaaaaa agaggtatga cgatgtccag acgcaatact gacgctatta
     3421 ccattcatag cattttggac tggatcgagg acaacctgga atcaccactg tcactggaga
     3481 aagtgtcaga gcgttcgggt tactctaaat ggcacctgca acggatgttt aaaaaagaaa
     3541 ccggtcattc attaggccaa tacatccgca gccgtaagat gacggaaatc gcgcaaaagc
     3601 tgaaggaaag taacgagccg atactctatc tggcagaacg atatggcttc gagtcgcaac
     3661 aaactctgac ccgaaccttc aaaaattact ttgatgttcc gccgcataaa taccggatga
     3721 ccaatatgca gggcgaatcg cgctttttac atccattaaa ccattacaac agctagttga
     3781 aaacgtgaca acgtcactga ggcaattatg aaaccgcttt tatccgcaat agcagctgcg
     3841 cttattctct tttccgcgca gggcgttgcg gaacaaaccc agcaaccgct cgttacttcc
     3901 tgtggcgatg tggtggttgt tcccccatcg caggaacaac caccgttcga tttaaatcac
     3961 atgggtacag gcagtgacaa atcggatgcg ctgggcgtgc cctattataa ccaacaagcc
     4021 atgtagtata ttcaggcccc ggcatatcgg ggcttgttaa cctcttacct tcaccgcttt
     4081 acgccactgc aaaccaaata cattgatata cagtccggcc ataatgagca cagcccctaa
     4141 gaattgcaga cctgttaagc gttcatccag cagtaacgct gcacttgcca gaccgactac
     4201 cggcaccagt aatgataatg gtgcgacacg ccatgtttca tagcgtccaa gcagcgtacc
     4261 ccaaatcccg tagccaacta tcgtcgctac aaatgccaga tacatcaacg ccagaatggt
     4321 ggtcatgtcg atagtgacca gactgtgaat catttttccg gagccatcga gaatgagcga
     4381 ggcgacaaaa aacggaatta tcgggattaa tgcgctccag acgaccagag acattaccgc
     4441 cggacgggat gaatgagaca tgatcttttt gttgaagata ttgccactcg cccaactaaa
     4501 tgccgctgcc agagtcaaca taaagccgag catcgccaca tgctgaccgt taaagctatc
     4561 ttcgattaac accagtacgc cagaaatcgc taacgcaatc cccgccagtt gcttaccgtg
     4621 cagccgttcg ccaaaagtaa acgcgccaag cacgatagta aaaaacgcct gagcctgtaa
     4681 gaccagcgag gccagtccag ccggcatacc gaagttaatg gcgcagaaga gaaaggcaaa
     4741 ctgtgcaaaa ctgatggtta aaccataccc cagcagtaaa ttcagcggta cttttggtcg
     4801 tgccacgaaa aagattgccg ggaaggcgac cagcataaag cgcaaaccgg ccagcatcag
     4861 cggtggcatg ttatgaagcc ccactttgat gaccacaaaa ttaagccccc atacaacgac
     4921 gactagtagc gccaacaccc catcttttcg cgacattcta ccgcctctga atttcatctt
     4981 ttgtaagcaa tcaacttagc tgaatttact tttctttaac agttgattcg ttagtcgccg
     5041 gttacgacgg cattaatgcg caaataagtc gctatacttc ggatttttgc catgctattt
     5101 ctttacatct ctaaaacaaa acataacgaa acgcactgcc ggacagacaa atgaacttat
     5161 ccctacgacg ctctaccagc gcccttcttg cctcgtcgtt gttattaacc atcggacgcg
     5221 gcgccacgct gccatttatg accatttact tgagtcgcca gtacagcctg agtgtcgatc
     5281 taatcggtta tgcgatgaca attgcgctca ctattggcgt cgtttttagc ctcggttttg
     5341 gtatcctggc ggataagttc gacaagaaac gctatatgtt actggcaatt accgccttcg
     5401 ccagcggttt tattgccatt cctttagtga ataacgtgac gctggtggtg ctcttttttg
     5461 ccctcattaa ctgcgcctat tccgtttttg ctaccgtgct gaaagcctgg tttgccgaca
     5521 atctttcatc caccagcaaa acgaagatct tctcgatcaa ctacaccatg ctcaatattg
     5581 gctggaccgt cggcccgcca ctcggcacgc tgttggtcat gcagagcatc aatctgccct
     5641 tttggctggc cgctatctgc tctgcgtttc ccatgttttt cattcaaatt tgggtaaagc
     5701 gcagcgagaa aatcatcgcc acggaaacag gcagtgtctg gtcgccgaaa gttttattac
     5761 aagataaggc actgttgtgg tttacctgct ctggctttct ggcttctttt gttagtggcg
     5821 catttgcttc atgcatttca caatatgtga tggtgattgc tgatggtgat tttgccgaaa
     5881 aggtggtcgc ggttgttctt ccagtgaatg ctgccatggt ggttacgttg caatattccg
     5941 tgggccgccg ccttaacccg gctaacatcc gcgcgctgat gacagcaggc accctctgtt
     6001 tcgtcatcgg tctgatcggt tttatttttt ccggcaacag cctgctattg tggggtatgt
     6061 cagctgcggt atttactgtc ggtgaaataa tttatgcgcc gggcgagtat atgttgattg
     6121 accatattgc gccgccagga atgaaagcca gctatttttc cgcccagtca ttaggctggt
     6181 tgggcgcagc ggtaaatcca ttagtgagtg gtgttgtact taccactctc ccgtcgtctt
     6241 cgctgtttat cattctggcg ttagtcatca ttgccgcgtg ggcgctgatg ttaaaaggta
     6301 ttaacgccag accccagagg caaccagcac tctgttgacg ttcgtgccgt tccactactg
     6361 gcacggcttc gctaatcatt attctgaggc aaaggcatag ccttcatctt cccagccggt
     6421 tatcccgccg agcatgattt taaccggctt tccgagtcga gccagtttga gtgccgcacg
     6481 gtctgcaccg ttacaatgtg gccccgcgca atacaccaca aatagcgtat cgtcgggcca
     6541 ttcggccatt ctttctgcgg tcatttgctt atggggtaaa tggagagcgc ctgggatatg
     6601 ccgacgcgcg aagtcctctt cacgtccaac cacatgaagt aaaacgaaat catcgccaat
     6661 ataagccaga gagtcatgaa catcggcgca atctgtttcg acactcaagc gcgcgagaaa
     6721 atgcgcctca gcaacttctg gcttcgctgt ggtaaattca gtgacataac gcatattgct
     6781 ttgtcctttt ggtttgttgt gttaacaata atgtaactaa tctacctttc actgaccgcc
     6841 tctcgatatg ccagaaatga gcaaaatgat gacaacgtta caacgtccgc tggtcattgc
     6901 actggcatat gacgggctat gtacttttga atttggcgtt gccgttgaaa tattcggcct
     6961 gccacgaccg gaactgggcg ataactggta tcgttttgct gttgctgcgg tagaaccagg
     7021 tgcgctacaa gccaccgggg ggatccacgt ggttgctgat ggtggactgg agttattgcc
     7081 caacgccgat atgattatta ttcctggctg gcgcggaata gatgccaaag tgccggagat
     7141 gctatgccag tcattgcgcg aggcacacca gcgcggtact cgcctggtat caatatgctc
     7201 tggcgtgttt gtccttgctg ctgcgggcct tcttcatcag cgaaaagcaa ccacccactg
     7261 gcgctacact gaacaactcc agcaccgtta cccttcaatc agcgtaagtg acaatgtgtt
     7321 atatcaggat gaaggacaag tgatgacctc cgccggaagc gccgcgggga tcgatctgtg
     7381 tttacatatc gtgcgcaaag attttggtca tgagattgct aataatgtcg cccggcgact
     7441 ggtcattcag ccacatcgcc agggggatca accacaaaat ctgacgcgcc caatggcctc
     7501 accgcgtgaa agccagacgt taggtgcctt attcgacttt ttgcagcaga atctggcaca
     7561 aacacatact gtgtcttccc tcgccgaacg ggtaaatatg agtccgcgca cttttttaag
     7621 acgttttgag gcgttaactg gcatgacgcc cgcccgctgg ctgctcaatg agcgcttaat
     7681 acgagtacag gattatctgc aaaattcggt ttttaacatc gagaaaattg ccgagctgac
     7741 gggttttggc aattcagcgt tattgcgtca tcactttcac caacgttttt tattatctcc
     7801 ttcgcaatat cgcaaaaaca gccgttcccc atcacaataa tcgcctgatt cagcctttgt
     7861 ttagataacc tctgctaata tctttctcat tgagataaaa attaaggtaa gcgaggaaac
     7921 acaccacacc ataaacggag gcaaataatg ctgggtaata tgaatgtttt tatggccata
     7981 ctgggaataa ttttattttc tggttttctg gccgcgtatt tcagccacaa atgggatgac
     8041 taatgaacgg agataatccc tcacctaacc ggccccttgt tacagttgtg tacaaggggc
     8101 ctgattttta tgacggcgaa aaaaaaccgc cagtaaaccg gcggtgaatg cttgcatgga
     8161 tagatttgtg ttttgctttt acgctaacag gcattttcct gcactgataa cggatcgttg
     8221 acacagtagc atcagttttc tcaatgaatg ttaaacggag cttaaactcg gttaatcaca
     8281 ttttgttcgt caataaacat gcagcgattt cttccggttt gcttaccctc atacattgcc
     8341 cggtctgctc ttccaatgac cacatccaga gtctcttcag gaaatgcgcg actcacacct
     8401 gctgtcacgg taatgttgat atgcccttca gaatgtgtga tggcatggtt atcgactaac
     8461 tggcaaattc tgacacctgc acgacatgct tcttcatcat tagccgcttt gacaataatg
     8521 ataaattctt cgcccccgta gcgataaacc gtttcgtaat cacgcgtcca actggctaag
     8581 taagttgcca gggtgcgtaa tactacatcg ccgattaaat gcccgtaggt atcattaacc
     8641 aatttaaatc ggtcaatatc caacaacatt aaataaagat tcagaggctc agtgttgcgt
     8701 aactgatgat caaaggattc atcaagaacc cgacgccccg gcaatcccgt caaaacatcc
     8761 atattgctac ggatcgtcag caaataaatt ttgtaatcgg ttaatgccgc agtaaaagaa
     8821 agcaccccct cctgaaaggc gtcgaaatgc gcgtcctgcc agtgattttc aacaatagcc
     8881 agcattaatt cccgaccaca attatgcata tgttgatggg cagaatccat tagccgaacg
     8941 taaggtaatt catcgttatc gagtggcctc agatgatcaa tccaccgacc aaactggcac
     9001 agtccataag aatggttatc cgttatttct ggcttactgg catctctcgc gaccacgctg
     9061 tgaaacatac tcaccagcca ctgatagtgg gcatcgatag ccttattgag atttaacaag
     9121 atggcatcaa tttccgttgt cttcttgatc attgccactc ctttttcaca gttccttgtg
     9181 cgcgctattc taacgagaga aaagcaaaat tacgtcaata ttttcataga aatccgaagt
     9241 tatgattcat ctctgagata acattgtgat ttaaaacaaa atcagcggat aaaaaagtgt
     9301 ttaattctgt aaattacctc ttcattatcg caaatgaaag gatgacaaat agcataaccc
     9361 agtacccaaa tggcccggta gttcaggcca tcaggctaat ttatttttat ttctgcaaat
     9421 gagtgacccg aacgacggcc ggcgcgcttt tcttatccag actgccgcta atgttgatca
     9481 tctggtccgg ctgaacttct cgtccatcaa aaacggtcgc aggaataaca acattaattt
     9541 caccactctt atcgcgaaaa acgtaacggt cctctccttt gtgagaaatc aaattaccgc
     9601 gtagtgaaac cgaagcgcca tcgtgcatgg tttttgcgaa atcaacagtc attttttttg
     9661 catcatcggt tccgcgatag ccatcttcta ttgcatgagg cggcggtggc gctgcatcct
     9721 gttttaaacc gccctggtca tctgccaacg cataagg
//
LOCUS       DBFIFI010000165         9732 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.165, whole genome shotgun sequence.
ACCESSION   DBFIFI010000165 DBFIFI010000000
VERSION     DBFIFI010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9732
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.165"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(63..2024)
                     /gene="topB"
                     /locus_tag="N7B39_003720"
     CDS             complement(63..2024)
                     /gene="topB"
                     /locus_tag="N7B39_003720"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HGU2423623.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRYLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLISRQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLIKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            complement(2029..3072)
                     /gene="selD"
                     /locus_tag="N7B39_003721"
     CDS             complement(2029..3072)
                     /gene="selD"
                     /locus_tag="N7B39_003721"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HGU2423624.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(3189..3740)
                     /locus_tag="N7B39_003722"
     CDS             complement(3189..3740)
                     /locus_tag="N7B39_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HGU2423625.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            3901..5757
                     /gene="sppA"
                     /locus_tag="N7B39_003723"
     CDS             3901..5757
                     /gene="sppA"
                     /locus_tag="N7B39_003723"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HGU2423626.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKADKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKAKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            5873..6583
                     /locus_tag="N7B39_003724"
     CDS             5873..6583
                     /locus_tag="N7B39_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310500.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HGU2423627.1"
                     /translation="MNKAPSLIAAIVLGLGISACGYFVGDGVKHLKTNNRYVNVRGLS
                     EKEVRADTAELTIAINFKGNVPGELFPKLEEAQKKIVAELNAQGINEKEIILGQWTSK
                     RTDSFYLKDDPTMPRYNADGSVTIKTHNVAAVEKVVAKLNELQVATDGAIAESKVAYR
                     FNGIGALRAEMIAAATKDARNAALQFATDSGSQVGSISDASQGVFQIFASGSDEDDPT
                     AINKTVRVVTTVTYALQD"
     gene            6715..7731
                     /gene="ansA"
                     /locus_tag="N7B39_003725"
     CDS             6715..7731
                     /gene="ansA"
                     /locus_tag="N7B39_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HGU2423628.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            7742..8383
                     /gene="pncA"
                     /locus_tag="N7B39_003726"
     CDS             7742..8383
                     /gene="pncA"
                     /locus_tag="N7B39_003726"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HGU2423629.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSALFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(8476..>9732)
                     /locus_tag="N7B39_003727"
     CDS             complement(8476..>9732)
                     /locus_tag="N7B39_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2423630.1"
                     /translation="GFLSYSGNVVLAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGG
                     FIGDYFGRRRAFRINLLIVGIAATGAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFT
                     EFIPATVRGKWSARLSFVGNWSPMLSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSG
                     KYFIESPRWLAGKGQIAGAECQLREVEQQIEREKSIRLPPLTSYQSNSKVKVIKGTFW
                     LLFKGEMLRRTLVAITVLIAMNISLYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAP
                     VGIFIAALIIDHFPRRLFGSTLLIIIAVLGYIYSIQTTEWAILIYGLVMIFFLYMYVC
                     FASAVYIPELWPTHLRLRGSGFVNAVGRIVAVFTPYGVAALLTHYGSITVFMVLGVML
                     LLCALVLSIFGIETRKVSLEEISEVN"
BASE COUNT         2403 a         2596 c         2431 g         2302 t
ORIGIN      
        1 aattttcttg atccgctgga tatttcagaa tatgactcga atagcacgaa agattcgctc
       61 gcttacgcta tcgccccgct tccggcttca tctgctggcg gacttttttt cgcactacgt
      121 ttacgcggtg cagccttttt cttatcagca ctgccaccac tgcccggagc cacaatgccg
      181 cgaaactgcc gcaccggagt acgtttggct tgatcaataa gctgatatag cgtccccacc
      241 agcggctgca taaagtcctg atagcgacac tgcttttcgc tgatttgcgt cagcaccgat
      301 tcccagtgcg cggtcatgtc cggtcgcgta gccatttctg gcagcgaatg gaataacgct
      361 tttccggcgt cggtggagtg gatatagcgc ccttttttga tgaggaaacc acgcttgaac
      421 aacagttcaa taatcccggc acgcgttgct tctgtcccca gaccatcggt cgcacgaagg
      481 atctttttca gatctttgtc ctgcacaaag cgcgcgatcc cggtcatcgc cgaaagcagt
      541 gttgcatcgg taaaatggcg cggcggctgg gtttgccgct ctaccacttc acctttttca
      601 cacagcaact catcgccttt cgccaccaca ggcaatggcg taccgtcgtt ttcttcatcg
      661 cgctctttgc tgcctaacag cgtgcgccag cctgcttcag caagaaaacg cgctttagcg
      721 acaaatttgc ctttggcaat gtccagttcg ataacacact tgcggaacac cgcatccggg
      781 cagaactgca tcagatactg acgggaaatc aggttataga ccttcgcttc gttctccgtc
      841 aggttgatcg cagaactccg tgcggtcgga atgatggcgt ggtgcgcatc gacctttttg
      901 tcatcccaac agcggttgcg tatatctgga tctaccactg gctgcggcaa cagatctggt
      961 gcatgaacgc tgatggcatt catcaccgcg tggcgtccgg caaaatgttc ttctggcaaa
     1021 tagcgacaat cggaacgcgg ataagtgatt agcttgtgcg tttcgtacag cttctggcag
     1081 atatcaagca cgttctgcgc gctcagacca aagcgttttg ccgcttcaat ctgcaacgcc
     1141 gaaagcgaaa acggcagcgg cgcggattct gattcccgtt tatcgttata gctggtgaca
     1201 atagccggtt gaccactaat gcggttaacc acatgctccg ccagtggacg atgtaacaag
     1261 cgcccttctt catcctggta cggttcacac gcttcgctcg gttgccagat agcggtaaac
     1321 cgctcatcgg caggtgtcac gatatgtgct ttgacttcaa agaagtcttt cgccacgaag
     1381 ttttcaatct cttcatcgcg gcgcaccacc agcccaagca cgggcgtctg cacgcgtccc
     1441 acggaaagca cgccctgata accggcattg cgaccgagaa tggtatacgc acgggtcata
     1501 ttgatgccgt acagccagtc ggcacgcgct cgcgccagcg cagaaacgca cagcggtaca
     1561 aactcactgt tggaacgaag acggtcgatc gcccgctcaa ccgcctgcgg gttcaggtcg
     1621 tttatcaagc aacgctgcac ctgctggcgc ttttccggtg ccagttgtag atagtccagc
     1681 acttcatcca ccagcagttg cccttcgcga tccgggtccc cggcgtgaac gatttcgctg
     1741 gcttcatgca ggtaccgttt gatgacgtta agttgtttgg tcacggaggg tcggggctgt
     1801 aattgccact tttccgggac aatcggcaaa tccgcaagat tccagcgcgc atagcggctg
     1861 tcgtaggcgt ctggctgcgc ctgctcaagc aggtgaccga tacaccaggt caccacctga
     1921 ccattaccgc actcgataaa gccatcgcct ttccggtgcg gtttgggcag gacatcagca
     1981 atggcgcgcg ccagactcgg tttttcggca ataaacaacc gcattgaatt aacggatctc
     2041 aaccatggca cgaccgccgc gtgctggcac cagttcgcca atcgccgtca gttcaatgcc
     2101 aaactcggcg gctgtagctt tgacctcatt ttcggcttcc ggcatgaccg ccagcagcaa
     2161 accgccagaa gtttgcggat cgcacagcaa atcgcgcact tcacgcggca tttcacccat
     2221 cagatgaccg tagctggcaa agttacgttc ggtgccgcca ggcactgcac ccaacttaat
     2281 gtactcttca acccccggca atttcgggat cgcgtcatag tcaacgcgcg cctgcacacc
     2341 tgcgccctga cacatttcgc tcaaatggcc cagcaggcca aagcccgtta cgtcggtcat
     2401 cgcttttacg ccttcaatgt tggcaaagga cgcgcctgcg atgttcatcc ggcacatcac
     2461 ttccgtcgcc agtccctgat gttctggttt cagtagtgat tttttctcag ccgtggtaag
     2521 aacgccgatc cccagcggtt tcgtcaggaa cagtttacat ccggcttgtg cggtactgtt
     2581 tttcttcacc cgctcggtcg gtacgatccc cgttaccgcc agaccaaaaa tcggctccgg
     2641 cgcatcgatg gagtgaccgc cagccagcgc aatacccgcc tgacggcatg catagcgtcc
     2701 accttcggtt acttcgcggg caatttccgg ggaaagtttg ttaatcggcc agccgaggat
     2761 cgcaattgcc ataatcggtt tgccccccat cgcgaagata tcgctgatgg cgttagtcgc
     2821 cgcaatgcgg ccaaaatcga aaggattatc aacgatcggc ataaagaagt cggtggtact
     2881 gataacgctg gtgccattgc ccagatcgta caccgccgca tcgtcgcggg tttcattacc
     2941 cacaagcaaa ttcggatcaa caaacttcgc ctgctcactg tgcaggatgg tttccaacac
     3001 ttttggggaa attttacagc cgcaaccagc tccgtggctg tattgggtca aacgaatcga
     3061 gttctcgctc atggacatct cctgtcaatg caatccgggt atggtaaccc tcattccgtg
     3121 aagtgataag tgagagtgtc tgaattcctg cgcctttgct cacaatccag acagtttcgc
     3181 gacaattatc agaaataagt gacaaacggc gtcgggtccg ggacgtttat cgacgtagat
     3241 gctttcagct gcggcgtgcc gaggtagaga aaaccgacaa ttttatcctg ctcacggcaa
     3301 ccgaatgctt cacgcactac cggactttca gttaatgcgc cactgcgcca gatgccgcca
     3361 aacccctggg caacggctgc catttgcatc gccatgaccg cgcatccggc agacatttcc
     3421 tgttcccatc gcgggacttt atgattttct tcgcatttcg ccaccaccgt gatgatgagc
     3481 ggtgcgcgga acggcgcatt acgggctttg tcgatagctt tgtcatcact accggcagca
     3541 atcgccccct gttccagtac ggcgctgaaa cgctcgcgcc cttccccttc aatcacaaaa
     3601 aaatgccacg gttgcatgga cttatggtcc ggcgcgcgca tacccgcacg caggatgttt
     3661 tgcagttgtt cacccgttgg tgcgggttca gccaggcggg aggcgctacg gcgattgatc
     3721 aatagttcga gtgcatccat ttgattaact cctgtcgtga tatttattca caaaattaac
     3781 acgagagtgg attttgttac agcacagtcc gcaattcctg ctgacaagtg ccggttgggt
     3841 cattacgata accacatcta ttgcgcctgt gacaggtgtg accttaagtt gggagaatac
     3901 atgcgaaccc tttggcgatt tattgccgga ttttttaaat ggacgtggcg tctgctgaat
     3961 ttcgtccgtg aaatggtact taacctgttc tttatttttc tcgtgctggt tggtgtgggg
     4021 atctggatgc aggtcagtgg tggtgattcg aaagaaacgg ccagtcgtgg tgcactgctg
     4081 ctggacattt ctggtgtgat cgtcgataaa cccgacagtt ctcagcggtt tagtaaatta
     4141 agccgccaac tacttggtgc cagttccgat cgtctgcagg aaaactcact gtttgatatc
     4201 gtcaatacca ttcgccaggc gaaggatgac cgcaatatca ccggtatcgt gatggatctg
     4261 aaaaacttcg caggcggcga ccaaccgtct atgcagtaca tcggcaaagc cctgaaagag
     4321 tttcgtgaca gcgggaaacc ggtttatgcc gttggcgaga actacagcca ggggcaatat
     4381 tatctcgcca gtttcgccaa taaaatttgg ctgtcaccgc aaggcgtggt ggatttgcac
     4441 ggttttgcta ccaacggtct gtactacaaa tcgttgctgg ataagctgaa agtttccacc
     4501 catgtgttcc gcgtgggtac gtataaatct gccgttgaac cgtttattcg tgatgacatg
     4561 tcaccggcag cccgcgaagc tgacagccgc tggattggtg agctgtggca aaactatctg
     4621 aatactgttg ccgctaaccg gcagatccct gctcagcagg tattccctgg cgcgcaaggg
     4681 ttgcttgagg gtttaaccaa aaccggtggc gataccgcga aatatgcact ggaaaacaag
     4741 ctggtcgatg cactggcatc gagtgcggaa atcgaaaaag cactgaccaa agagttcggc
     4801 tggagtaagg ctgataaaaa ttatcgcgcc atcagttatt acgattacgc attgaaaacg
     4861 ccggcagata ccggtgacag catcggtgtc gtcttcgcta atggcgcaat tatggatggc
     4921 gaggaaactc aggggaatgt tggcggtgat accactgcgg cacaaatccg cgacgctcgc
     4981 cttgacccga aagtgaaagc gattgtcctg cgtgttaata gcccaggcgg cagcgttacc
     5041 gcgtctgaag tgattcgcgc tgaactggca gcagcccggg cagcgggtaa gcctgtggtt
     5101 gtatcgatgg gcggcatggc ggcatctggt ggttactgga tttccacgcc agctaattac
     5161 attgtggcta accccagcac cctgaccggt tctattggta tcttcggcgt gatcaccacc
     5221 gtagaaaata gtctggattc gattggtgtt catactgatg gtgtctcaac ttcaccgctg
     5281 gcggatgttt ctatcaccag ggcactgccg ccggaagcgc agcagatgat gcagttaagc
     5341 attgagaatg gctataaacg ctttatcacg ctggttgctg atgcgcgtca ttcgacgccg
     5401 gagcagattg ataaaatcgc ccagggccac gtctggaccg gtcaggacgc aaaagctaac
     5461 gggctggtcg atagtctcgg ggatttcgat gatgcggtcg ccaaagcagc agagctggca
     5521 aaagcgaaac agtggcatct ggaatactac gttgatgaac cgaccttctt cgacaaagtg
     5581 atggacaaca tgtctggttc tgtccgggca atgttgccag atgcgttcca ggccatgtta
     5641 cctgcaccgc tggcctcggt agcctctacc gttaaaagtg aaagcgacaa gctggcggcg
     5701 tttaacgacc cacaaaaccg ttatgcgttt tgcctgacct gcgccaacgt gcgttaaatc
     5761 ctgcaaaaat gtggccgata tgctgtcggc cacactcctg aatcggactg tctttcactc
     5821 cctctctcca ctacattccc ttgctcattt agcatctgat atggagtcat cagtgaacaa
     5881 ggccccctct ttaattgccg ctatcgtcct cggactgggg attagcgcct gtggttattt
     5941 tgttggcgat ggtgttaaac atttaaaaac caacaaccgt tatgtcaatg tgcgcgggct
     6001 ttctgaaaaa gaagttcgcg cagatacggc ggaattaaca attgcgatta actttaaggg
     6061 taatgttccc ggcgaactgt tcccgaaact ggaagaagcg cagaaaaaaa ttgttgccga
     6121 actcaatgct caggggatta acgagaaaga gataattctg ggccaatgga caagcaagcg
     6181 aacagattcc ttttatctga aagatgatcc cacaatgcca cgctataacg cagacgggtc
     6241 agtgacgata aaaacccata atgttgctgc tgtcgaaaaa gtcgtcgcga aattaaacga
     6301 actgcaagtc gctacggatg gcgctatcgc tgagagcaaa gtcgcctatc gctttaatgg
     6361 tattggcgca ctgcgcgctg agatgattgc tgccgccacc aaagatgcac gcaacgccgc
     6421 actacaattt gcaacggact caggtagcca ggtggggtca attagcgatg cctcacaagg
     6481 cgtattccag atctttgcca gtggcagtga tgaagatgat cctacggcga ttaataaaac
     6541 ggtacgcgta gtcacgaccg taacctatgc gttgcaggat taaccacttt tctccggcgg
     6601 gacactaatc cctccgtcct gccggacttt tctcatatcc acagagtatc caggtgcgct
     6661 cgctgcccct cacgtatata cttttgctct ttcgatatca ttcatatcaa tatcatgcaa
     6721 aagaaatcaa tttacgttgc ctacacgggc gggaccatcg ggatgcagcg ttccgagcag
     6781 ggttatatac cggtgtcagg tcatctacaa cgccaactgg cgctgatgcc ggaattccat
     6841 cgcccggaga tgccagattt caccattcat gaatatacgc cgctgatgga ttcttcagat
     6901 atgacgccgg aagactggca gcatattgct gaagatatta aagcgcacta tgacgactat
     6961 gatggttttg tcattctgca cggcaccgac acgatggcgt ataccgcctc tgcgctgtcg
     7021 ttcatgctcg aaaatctcgg taaaccggtc attgtgacag ggtcacaaat cccgctggct
     7081 gagttacgct ctgacggaca aattaatctg ctgaatgcgt tgtacgttgc ggcgaattat
     7141 ccgatcaacg aagtaacact cttttttaat aaccgactgt atcgcggcaa ccgcactacc
     7201 aaagcccatg ccgatggttt tgatgcgttt gcctctccaa accttcctcc gttactggaa
     7261 gcaggtatcc atattcgtcg tttgaatacg ccacccgccc cgcacggtga aggggaattg
     7321 atcgtacatc caatcacccc acaaccaatt ggcgtagtga cgatttatcc agggatttct
     7381 gctgacgtcg tgcgcaattt tctgcgccaa ccggtgaaag cattgattct gcgctcctat
     7441 ggcgtgggta atgcgccaca aaacaaagcc ttcctgcagg aattacaaga agccagcgat
     7501 cgcggtattg tggtggtcaa cctgacacaa tgtatgtccg gtaaagtgaa tatgggtggt
     7561 tatgccaccg gtaacgccct cgcccatgct ggcgttattg gcggtgcaga tatgactgta
     7621 gaagccacgc taaccaaact gcattacctg cttagccagg aactggatac tgaaaccatt
     7681 cgcaaggcca tgagccaaaa cctgcgcggc gaactgacgc cggatgatta aggagactgc
     7741 aatgccccct cgcgccctgt tactggtcga tttacaaaat gatttctgtg ctggtggcgc
     7801 gctcgccgtg ccggaaggtg acagtacggt ggatgtcgct aaccgcctga ttgactggtg
     7861 ccagtcgcgc ggtgaagcgg ttatcgccag tcaggactgg cacccggcga atcacggcag
     7921 ttttgccagc cagcacggtg tagagcctta tacgccaggc caactcgacg gtttgccaca
     7981 aaccttctgg ccagatcact gtgtgcagaa cagtgaaggc gcacaattac atccgttact
     8041 gaaccaaaaa gcgatcgcag ctgtgttcca taaaggcgaa aatcctttag ttgacagtta
     8101 cagtgccctt tttgataacg gccgtcggca gaaaacctct ctcgatgact ggttacgcga
     8161 tcatgaaatc gatgaattga tcgttatggg cctggctact gactattgcg tgaagtttac
     8221 cgtgctggac gcgttacagt taggttataa ggtaaacgtg attaccgatg gttgtcgtgg
     8281 cgtgaatatc cagccccagg acagtgcgca cgcgtttatg gagatgtcag cagctggggc
     8341 aacgctatat acgctggcag actgggaaga gacgcagggg taattttacg ctggcctaca
     8401 attctgtact ggcattgtag gccaaataaa acacgtcagt ggcacatctg gcaattgatg
     8461 ccatcaacga aagatttaat ttacctcaga aatctcttcc aacgacacct tccgcgtttc
     8521 gatgccaaaa atggagagaa ccagcgcaca gagcaataac atgacaccaa gtaccataaa
     8581 caccgtgatc gacccataat gtgttaataa tgccgcaacg ccatagggcg tgaagactgc
     8641 gacgatccgt ccgacggcat taacgaaacc cgaaccgcgc aggcgtaaat gcgttggcca
     8701 aagctccggg atataaaccg ccgacgcgaa gcaaacatac atgtataaaa agaagatcat
     8761 caccaggcca tagattaaaa tcgcccactc tgtagtctga attgaataga tatagcctaa
     8821 cactgcaata ataataagta aggtggagcc aaataaccga cgaggaaaat gatcaataat
     8881 taatgccgca ataaatattc ctaccggagc gccaatcata ataacagcgg tcattaatat
     8941 tgatttatcg acatcaatgc cggagttaac aaatatggtc ggtatccata cggtgatggt
     9001 ataaagcgaa atgttcattg caattaaaac agtaatcgcg actaatgtac gtcgtaacat
     9061 ttcaccttta aacaggagcc agaaagtacc cttgattact ttaaccttgc tgttgctctg
     9121 atacgaagta agcgggggta aacgaatact cttctctctt tcaatttgct gctctacttc
     9181 acgaagttgg cattctgcac ctgcgatttg ccctttccct gccagccatc gtggcgactc
     9241 aataaagtat ttacctgaga gaaaccaggc taacagtatg ccaataccac ccaacagaaa
     9301 cattatccgc caactaaaaa aagcgataac caccacacct atcgccgcag acagcatggg
     9361 cgaccagtta ccaacaaatg agagccgcgc ggaccatttt ccacgcaccg tcgcggggat
     9421 aaactccgta aatgaggcat agccaaccat aatcagcgcc cccattcctg ttcccatcag
     9481 gaagcgaaag aagataagcc agtacatatc agggacaaaa gcggcccctg ttgcagcaat
     9541 accgacgatg agaagattta tgcgaaacgc cctgcgccgc ccaaagtaat caccgatgaa
     9601 cccaccggta agagagccga tgaaataacc aaacattaat gccgaggtaa aggcggcatt
     9661 gaggaaatta tttgaccatc cattgcttac cagctttgct aagacgacat taccggagta
     9721 actcaaaaac cc
//
LOCUS       DBFIFI010000166         9655 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.166, whole genome shotgun sequence.
ACCESSION   DBFIFI010000166 DBFIFI010000000
VERSION     DBFIFI010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9655
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.166"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..683
                     /locus_tag="N7B39_003728"
     CDS             <1..683
                     /locus_tag="N7B39_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2423631.1"
                     /translation="VLFNPRQKALLYCLVLSLNSVNDVYAGLWQSCYTPDFNTQRWSR
                     DLPQLPQDFFAKLTPEWQRNCALRSDYSRRQALVEIDVLVAQALGLTLEELLTIYRVQ
                     FPVMRQYEADTWYDQNGRIIFTPSKGLVGVGLPRTARKADLKNGFVFNVDSPEWTGGD
                     CTDQAIGWDDVKHLKTGTVSVTFDDYTRSDEGERRTVTWQAPFIKPDREDDYKVAWAF
                     FAQDKESA"
     gene            683..7024
                     /locus_tag="N7B39_003729"
     CDS             683..7024
                     /locus_tag="N7B39_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGU2423632.1"
                     /translation="MLPSVVSRQVADSVAAFLRAAFPLNSPLFNGENNDNVSMLEQFL
                     AQPETLLKGPYLSAQLPFRKSDLPLNFFPNLTLPFPPHAHQARAFQRLGSDAPQPTLV
                     ATGTGSGKTECFMFPLLNHCAGASQAGVKAIIIYPMNALATDQASRFAKTIASDPQLH
                     GKVTVGLFVGDSEIEPSKKMSAEKVITCKHTLRESPPDILLTNYKMLDYLLMRPGDQP
                     LWRYNQPGSLRYLVVDELHTFDGAQGSDLACLVRRLKHHIGVDNQRFACVGTSATVGD
                     ELGQLLDYAKTIFDQPFTDDAVIREDRYTAAEYLQNYAIAYSQYPGPEVASALDPQSY
                     ATPVAYLNGQIPLWFPDSDLQLPDDLESDDGREKRIALGSLLRRHSVMHVLLHDLQGG
                     ILSERECLENLQVMLAESADHARRVLQSILALIAQARLEVPETEHDRQKRLQAKKARP
                     VLPFVQLRSQLWLREMRRLVASVAKTPRLVFADDVAVEDREHYLPVIHCRDCHATGWA
                     SLRHGQSVQLETDLDGIYRQFFEKGRSIVLAFPDNNEKAVSGAHQKLCPTCLKLNRQS
                     SVQCGHCGHTELLQVLIPDMLKERKDELEFANECPYCNSKQGISILGSRAASLSAVMI
                     HQLYGSQFNEHKKLITFSDSVQDAAHRASFFTARTWPLMIRGQIASVLSEKETLPLDA
                     FAARVCQQTRERSPDDAHFAANVIAPNMQWLNDYQTLKNEGKLAENSDLPELVSLRLD
                     WEVYSEFTLRARIGRTLEATGYAVAGVDKDRFNPAITALHQQLCEELGDEMSGVSVEQ
                     VAHLALGVIWYQRQAGAVMHPAFESYRRDGTTFMMTQKERTSRYMPSIGPKTRKPAYA
                     SFEKINGFHRLIGAKSNPSWYQHWINRTLSNGNNLFISSVAETVLRRLFTALKIAGVV
                     KDFDTKGREAWGLVPSALVVSRDVVQLNCSCCREVVRAPADQGWQWRNAPCLSLRCEG
                     RYQEVDNTATWRWENMDIARVQGAEHTGLLSREDREVTEKSFYRGNQPWNINLLSATP
                     TLEMGIDVGDLSTVLLCSVPPAQANYLQRIGRAGRKDGNALNITVAEGNPHDQFFFEE
                     PLEMMQGQVQAPGVFLNATAILERQLAAFCMDNWVKTGVPESAICKNVKQMLDELEFG
                     RKSGFPYNLLRYIEQHHAEIAAQFTAIFPDLAAETRQQLLSYLQGAPGQRSLVQRIEE
                     ALKLLVEDRKSLRSRIDKLKRSIDKLENAPRDQNFDSDMRELTSERQALMALVNQINN
                     KQTLNFLTDEGLLPNYAFPEAGITLRSVLWRRKEGGEAREYQNTTFEYERPASTALAE
                     LAPLNNFYAGGHKVEIEQIDLKVSEPENWRICSHCNYSENIDQTGDQHKYCPKCGTPG
                     WADAGQKTTLLKLRQVYARASARDSQISDESDSREPAFFQRQLLVSFEKEDVSAAYAI
                     EEGEVPFGFEFLSKVTLRDINFGKMADDANELMIAGEAKKRTGFKVCLGCGMVQRPRD
                     HEPRHDLSCKYRTEPEKAKFEDYLYLYRQLESEALRILLPVSSYSNDRVVEASLGAAI
                     QLGLKHYFKGNVDHLKGVVYREPENEGESWRQYLVIYDTVPGGTGSLKELMRTPDNLL
                     KLLELAYQAIVECSCNNDTHKDGCYRCVYAYRDRGRMKYVSRDQARLLLAKILQARDS
                     IRVIDSIKNISLEAMMGSELEKRFISCLQENKNLIVSRSYAHQGAGWIINTRSEPMMS
                     WHLKAQVDLGAKEAVGIPCRPDYVLYPLMQSDAIKPVAIFLDGFAFHKNSVAEDVQKR
                     QAIRDSGNFWVWTVTWADLKEPGLKHVQDVLGLGHNPDMKQPKFYNLFHDTNFAALEA
                     SFRERNSFALLLDYLADPGGRTQLWQRMAAAHAWVWLDVKKSQDAGTKQKYAYEMQEN
                     APAWRLAELLPDEPFVFGGLLDSCNSSQQFIELASVLPQQAIKPTTSVAQMRSWLRLH
                     ICFDDRYTQDDGYEAGLNGFWRLVNLLQFLPDMTFTSRKAVHLPQESVDTKVSATPEA
                     VPETDESWAEIIEFGLLSAEEIALLQSHSLSAPTLGYELQNDEGEIIAEADLAWPSQK
                     WALIIDNQEFIPLFTTRGWHVAFGPIDENTLQHLSGGDK"
     gene            7021..9135
                     /locus_tag="N7B39_003730"
     CDS             7021..9135
                     /locus_tag="N7B39_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HGU2423633.1"
                     /translation="MTVQTPKVALSDGFLGAFARIPKAQQKKVQEFISKFRQDPTSNG
                     LNYEKIHDARSKNVHSVRIDQTYRGIVLKPEQGALYMLMWVDKHDEAYDWARRHDCSI
                     HPVTGAIQVIDISYIKPAAETVVDKPKLFAAYSAEQILALGVPPVFIDQVMALTDEAG
                     LNQLESIMPAEAWEPLHWLAEGLDYQEVLEEFNDHRDEPVDTNDFMEAIERSKRRFHV
                     VENEQELLQMLNAPLEKWRVFLHPSQRKLVESPANGPVRVLGGAGTGKTVVAMHRARW
                     LSQRLADKPGKKVLFTTFTRNLAADIRANLQRLCTREEMARIDVVNIDAWMSDQLKRH
                     GYDFRVVYDSDEGRRKCWNYALQQAPAELGLPDNFYAEEWQRVVQPNAVYTREEYLKV
                     SRVGRGTALSRIQRAKIWPVFEEFRAQMARAKLREMGDAMHEAIVLFKEKQVQLPYSS
                     IVVDEAQDIGAPAFTLIRSLVPESPNDLFIVGDGHQRIYRNKVVLGQCGINVRGRRSK
                     KLKINYRTTEETRQFAVGLLTGVKVDNLDGEADTSNDYLSLLHGEKPMITHAADFKEE
                     AATLVKQIQALLANQVRSQDICITARTKHACDRYASALNDAGIETFNLGNDSGDSDAR
                     PGVRVATMHRIKGLEFQYVFLVGINEGVVPEIKALASDDPVEQRDALFNERALLHVAA
                     TRAVKGLFVSSSGVPSPLLVAD"
BASE COUNT         2397 a         2334 c         2628 g         2296 t
ORIGIN      
        1 acgtattatt caatccgaga caaaaggcat tattatactg cttagtatta tcattaaatt
       61 ctgtaaatga tgtatatgct ggcctttggc aatcctgcta caccccagac ttcaacaccc
      121 agcgttggag ccgcgatctc ccgcagctcc cccaggattt cttcgccaaa ctgaccccag
      181 agtggcagcg taactgcgct ttacgctctg actacagtcg tcgtcaggcg ctggtggaaa
      241 tcgacgtatt ggtggcgcag gcgctggggt taactctcga agagctgctt accatttatc
      301 gcgttcagtt cccggtgatg cgccagtacg aagcggatac ctggtacgat caaaacggtc
      361 gcattatctt taccccaagc aaagggctgg tgggcgttgg cttgcctcgc accgcgcgta
      421 aagctgacct gaaaaacggc tttgtcttta acgtcgacag cccggagtgg accggcggtg
      481 actgcaccga tcaagctatc ggttgggatg atgtcaaaca tcttaaaacc ggtaccgtca
      541 gcgtcacctt tgatgattat acccgcagcg acgaaggtga gcgccgtacc gtcacctggc
      601 aggctccgtt tatcaagcca gatcgcgaag atgactacaa agtggcctgg gcgttctttg
      661 cacaagataa ggagagcgcc tgatgttgcc gtccgttgtc agtcggcagg tcgccgacag
      721 cgttgccgcc tttttacggg cggcgttccc gctaaacagc ccgctgttta acggtgagaa
      781 taacgataat gtctcaatgc tggagcaatt tctggcccag ccggaaacgc tgctgaaagg
      841 accatacctt tccgcccagt tgccgtttcg taaaagtgac ttaccgctga attttttccc
      901 taatctgacg ctgccgttcc cgccgcacgc gcatcaggcc cgggcatttc agcgtctggg
      961 aagcgacgcg ccacagccga cgctggtcgc aaccggtacc ggctccggta aaacagaatg
     1021 ttttatgttc ccgctgctca accactgcgc gggcgcgtca caagccgggg ttaaagcgat
     1081 tatcatctac ccgatgaacg ccctggcaac cgatcaggcc agccgtttcg ccaaaaccat
     1141 cgccagcgat ccacaactgc atggcaaggt gaccgtcggc ctgtttgtcg gcgacagcga
     1201 aatcgaaccc agcaaaaaaa tgtcggcaga aaaggtcatt acctgtaagc acacgctgcg
     1261 cgaaagcccg ccggacatcc tgttgaccaa ctacaaaatg ctcgactacc tgctgatgcg
     1321 ccctggcgat cagccgctct ggcgttacaa ccagcccggt tcattgcgtt acctggtcgt
     1381 ggatgaattg catacgtttg atggcgcgca agggtcggat ctggcctgtc tggtgcgacg
     1441 tttgaagcat cacataggtg tggataacca acgattcgcc tgcgtcggga catcagctac
     1501 cgtcggcgac gaactggggc aactgctcga ttacgcaaaa acaatctttg accagccgtt
     1561 taccgatgac gccgttattc gcgaagatcg ctacacggca gcggagtact tgcagaatta
     1621 cgccattgcg tacagtcagt atccggggcc ggaggtcgct tcggccctgg acccgcagag
     1681 ttacgctacc ccggtagcgt atcttaacgg gcaaatccct ttgtggttcc ccgacagcga
     1741 cttgcaactc ccggacgatc tggagagcga cgacgggcgt gaaaagcgta ttgcgctggg
     1801 gtcactgtta cgccgccaca gcgtgatgca tgtcttactc catgatctgc aaggcgggat
     1861 cctctctgaa cgagagtgtc tggaaaatct ccaggtcatg ctggcagaaa gcgccgacca
     1921 tgcccgacgc gtgctgcaaa gcattctggc actgatcgcg caggcacgtc tcgaagtacc
     1981 ggaaacggaa cacgatcggc aaaaacgtct ccaggcgaag aaagcgcgtc cggtgctgcc
     2041 gtttgtgcaa ctgcgctccc agttgtggct tcgtgagatg cgtcgtctgg tcgccagcgt
     2101 ggcgaaaacg ccccgactgg tgtttgccga tgatgtggcg gtggaagatc gcgaacacta
     2161 tttgccggtt atccactgcc gtgactgcca cgcaactggc tgggccagcc tgcgtcatgg
     2221 gcaaagcgtt cagttggaaa cagacctcga cggcatttat cgccagttct ttgaaaaagg
     2281 gcgctccatc gtccttgctt tcccggataa caacgaaaaa gcggtcagtg gcgcacacca
     2341 gaaactgtgt cctacctgtc tgaaactgaa ccgacagtcc agcgtccagt gtggacattg
     2401 cggtcatacg gaactgttgc aggtactgat cccggatatg ctcaaagagc gtaaggacga
     2461 gctggagttt gctaatgaat gcccgtactg caacagcaaa caggggattt cgattctggg
     2521 gtcacgggct gccagtctgt cggcggtgat gattcaccag ctttatggca gccagttcaa
     2581 cgaacataaa aagctgatta cgttttctga ctcggtacaa gacgccgcgc atcgtgccag
     2641 cttctttact gcccgtacct ggccgctgat gattcgtggg caaatcgcca gtgttcttag
     2701 cgaaaaagag acattaccgc tggatgcgtt cgcggcgcgt gtttgccaac aaacgcgtga
     2761 acgttcaccg gacgatgctc attttgcagc aaacgtcatc gcaccaaata tgcagtggct
     2821 gaatgattat cagacgctga aaaatgaagg caaactggcg gagaactccg atctgccgga
     2881 gctggtttcc ctgcgcctcg actgggaggt gtacagcgaa tttaccctgc gcgcgcgtat
     2941 cggacgtacg ctggaagcca ccggttatgc cgttgccgga gtggataaag atcgcttcaa
     3001 tccggcaatt accgccctgc atcaacagct ttgtgaagag ctgggtgatg agatgtctgg
     3061 tgtgagcgtc gagcaggtag cgcacctggc gctgggggtt atctggtatc aacgccaggc
     3121 cggggccgtt atgcatccgg cgttcgaaag ttatcgccgt gatggcacga cctttatgat
     3181 gacgcaaaaa gagcgtacct cgcgctatat gccttctatc ggtccgaaaa cgcgtaaacc
     3241 ggcttatgcg tcgtttgaaa aaattaatgg tttccatcgc ctgattggcg cgaagtctaa
     3301 ccccagttgg tatcagcatt ggatcaatcg cacgctgagt aacggcaata atctgtttat
     3361 cagtagcgtt gcggaaacag tgttacgacg cctgtttacg gcgttgaaaa tagccggagt
     3421 agtaaaagat tttgacacca aagggcgtga agcctgggga ctggtgcctt cggcgctggt
     3481 ggtgagccgt gacgtcgttc agcttaattg ctcatgttgc cgtgaggttg tccgggctcc
     3541 ggccgatcaa ggctggcagt ggcgtaatgc gccttgtctc tctttacgtt gtgaaggtcg
     3601 ttaccaggaa gtcgacaata cggcgacctg gcgttgggag aacatggaca tcgcgcgcgt
     3661 acagggcgca gaacacacgg ggctgcttag ccgtgaagat cgcgaagtca ccgaaaaatc
     3721 gttctaccgc ggcaaccaac cgtggaatat caacctgttg tcggcaacac cgacgctgga
     3781 gatgggtatt gacgttgggg atctttccac cgttttactc tgttctgtcc cacctgctca
     3841 ggccaactac ttgcagcgta ttggtcgcgc cggtcgtaaa gatggtaacg cgttaaacat
     3901 taccgtggca gaagggaacc cgcacgatca gtttttcttt gaagaaccac tggagatgat
     3961 gcagggccag gtgcaggctc ctggcgtctt ccttaatgca acggctattc tcgaacgcca
     4021 gctggcagct ttttgtatgg ataactgggt gaaaaccggt gtaccagaat ccgctatctg
     4081 taaaaacgtc aaacagatgc tggatgagct ggagttcggg cgtaaatcgg gttttccgta
     4141 taacttactg cgatatatcg aacagcatca tgcggagatt gctgcgcaat ttacggcaat
     4201 cttccccgat cttgccgcag aaactcgcca acagctgttg tcttatcttc agggcgcgcc
     4261 aggacaacgc tcgctggtgc agcgtatcga agaagcgtta aaactgctgg tggaagatcg
     4321 caaatctttg cgctcacgca tcgataaact caagcgcagt atcgataaac tggagaacgc
     4381 gccgcgcgat caaaactttg atagcgatat gcgcgagtta acttcggaac ggcaggcgct
     4441 gatggcgctg gtcaatcaaa tcaacaataa gcaaacgctg aatttcctca cggatgaagg
     4501 tctgctgcct aactacgcct tcccggaagc cggtattacg ttacgttctg tgctctggcg
     4561 gcgtaaagaa ggcggggaag cacgtgaata tcaaaacacc actttcgaat atgaacgccc
     4621 ggcatcaacg gcgctggctg agctggctcc gctgaataat ttctatgccg gtgggcataa
     4681 agttgaaatt gagcagatag acctgaaggt ctctgagccg gagaactggc gtatctgtag
     4741 ccactgtaac tacagcgaaa atatcgacca gacgggcgac cagcataaat attgcccgaa
     4801 atgtggcaca ccggggtggg ccgatgccgg acaaaaaacc acgctgctga aacttcgcca
     4861 ggtttatgcc cgcgccagcg cacgtgatag ccagatcagc gacgagagcg acagccgtga
     4921 acctgctttc ttccagcgtc agctattggt tagtttcgaa aaagaagatg tatcggcggc
     4981 atacgcgatt gaggaaggcg aagttccgtt tggttttgaa tttttaagca aagtcaccct
     5041 gcgggatatc aacttcggca aaatggcgga tgatgccaat gagttgatga ttgccggaga
     5101 agcgaagaag cgaaccgggt ttaaagtttg cctcgggtgc ggcatggtgc agcgtcctcg
     5161 cgatcatgaa ccacgtcatg atttgagctg caaatatcgc acggaaccgg aaaaggcgaa
     5221 gttcgaagat tatctctacc tctatcgcca gctggagtca gaagcgttgc gtattctgct
     5281 cccggtaagc agttacagta atgaccgtgt cgtggaggct tctcttgggg cggcgatcca
     5341 gctcggtctt aaacattact tcaaggggaa tgtcgatcac ctgaaaggcg ttgtgtaccg
     5401 cgagccggaa aatgaaggcg aatcctggcg gcagtatctg gtgatttacg ataccgtccc
     5461 gggggggact ggttctctta aagaactgat gcgcactccc gacaatttgc tgaaattact
     5521 tgaactggcc tatcaggcaa tagtggaatg cagttgcaac aatgatacgc ataaagatgg
     5581 ttgttatcgt tgcgtttacg cttaccgtga ccgtgggcgc atgaagtatg tctcccgcga
     5641 tcaggcgcgt ctcttgctgg cgaaaatttt gcaggcaaga gattcgatac gggtaattga
     5701 ttccatcaaa aacatttccc tcgaagccat gatgggcagt gagctggaaa aacggtttat
     5761 tagctgcctg caagaaaata aaaacctgat tgtcagtcgc agttacgcgc atcagggcgc
     5821 tgggtggatt attaacaccc gcagcgaacc gatgatgagc tggcatctta aagctcaggt
     5881 ggatttaggc gctaaagagg cggtggggat cccttgtcgc ccggattacg tgctatatcc
     5941 gcttatgcag tctgatgcca ttaaaccggt ggctattttc cttgatggct ttgcgttcca
     6001 caaaaatagc gtcgcagagg atgtgcaaaa acgccaggcg attcgggata gcggtaattt
     6061 ctgggtgtgg acagtcacgt gggccgatct caaagagcca gggcttaaac atgttcagga
     6121 tgtcctggga cttgggcata acccggatat gaaacagcct aagttttata acctgtttca
     6181 tgataccaac tttgcggccc ttgaagcgtc gttccgggaa cgcaacagtt ttgcactgct
     6241 gctggattat ctcgccgatc cgggcggaag aacgcagtta tggcagagaa tggctgctgc
     6301 acatgcctgg gtgtggttgg atgtgaagaa gtcacaggac gcgggaacga aacaaaagta
     6361 tgcctatgaa atgcaggaaa acgcgccagc ttggcggcta gcagagcttt tgccagacga
     6421 accgtttgtc tttggtggtt tactcgatag ctgtaactca tcgcagcaat ttattgagct
     6481 ggcttcggtg ttaccacaac aagccattaa accgacgacc agcgtcgcac aaatgcgcag
     6541 ctggctgcgt ttgcatatct gttttgacga tcgctatacg caggatgacg gttatgaagc
     6601 gggactcaat ggcttctggc gactggtaaa cctgttacag ttcctgccgg atatgacctt
     6661 taccagccgt aaagccgtgc atttaccgca ggaaagcgtt gacactaaag tttcggcaac
     6721 gccagaggca gtaccagaga ctgatgagag ttgggctgag attattgagt ttggcttatt
     6781 aagcgctgaa gagatcgcgt tgcttcagtc tcattctctc tctgcgccga ctctcggcta
     6841 cgaattgcaa aatgatgaag gtgaaattat tgcggaagcc gatctcgcct ggccatcaca
     6901 aaaatgggca ttgattattg ataatcaaga atttattcca ttattcacaa ccaggggttg
     6961 gcacgttgca ttcgggccaa ttgacgaaaa tacattgcaa catctgtccg gaggtgacaa
     7021 atgacggtgc aaacgcccaa ggtggcactt tctgatggtt ttctgggcgc attcgcgcgg
     7081 atcccaaagg cacagcaaaa aaaggtgcag gaattcatct ccaaattccg tcaggacccg
     7141 accagtaatg gacttaacta tgaaaagatc cacgatgcgc gcagtaaaaa tgtccattcg
     7201 gtgcgaatcg atcaaactta tcgcggcatt gttctgaaac ctgaacaggg tgccctgtat
     7261 atgctgatgt gggtggataa acacgacgaa gcatatgact gggcgcggcg gcatgattgt
     7321 tcaattcacc cggttaccgg cgcgattcag gtcattgata ttagctatat caaaccggca
     7381 gcggaaacag tggttgataa accgaaactc ttcgctgctt acagcgcgga acagattctg
     7441 gctctgggtg tgccgcctgt ctttattgat caggtgatgg cgcttaccga tgaagccggg
     7501 cttaatcaac ttgaaagcat aatgccagca gaagcctggg aacctctgca ctggctggca
     7561 gaagggctgg attaccagga agtgctggaa gagtttaacg atcatcgtga tgagcctgtt
     7621 gataccaacg actttatgga ggcgattgaa cgaagtaaac gtcgcttcca tgtcgttgaa
     7681 aatgaacagg agctgttgca gatgcttaat gcgcctttag aaaaatggcg tgtatttctg
     7741 catccgagcc agcgaaaatt ggtggagtcg cccgctaatg gtccggtacg agtactgggc
     7801 ggtgcaggta cagggaaaac ggttgttgcg atgcaccgtg cgcggtggtt atctcagcgt
     7861 ctggcagata aacccggtaa gaaagtactt ttcacgacct ttacgcgtaa cctggcggca
     7921 gatattcgcg ctaatctcca gcggttgtgt acgcgagaag agatggcgcg tattgacgtc
     7981 gttaacattg atgcctggat gagcgaccaa ctaaaacgcc acggttatga tttccgcgtg
     8041 gtatacgaca gcgatgaagg gcgacgcaaa tgctggaact atgccttgca gcaggcacct
     8101 gccgaactgg ggctacctga taatttctat gctgaagagt ggcaacgcgt tgtccagcca
     8161 aatgcggttt acacccggga ggaatacctc aaagtcagcc gggttggacg tggtacagcg
     8221 ttatcccgta ttcaacgggc gaaaatctgg ccagtctttg aagaattcag agcacaaatg
     8281 gcgcgtgcca aactgcgcga aatgggcgat gccatgcatg aggctattgt tctgtttaaa
     8341 gaaaagcagg tccaactacc ttatagcagt attgtggtcg atgaagctca agatatcggt
     8401 gccccggcat ttactcttat tcgcagcctg gttcccgagt cgcccaacga tctgtttatt
     8461 gtcggggatg ggcatcagcg tatttatcgc aataaggtcg ttcttggtca gtgtggtatt
     8521 aacgtccgtg gtcgtcgcag taaaaaattg aagattaact atcggaccac agaagagacg
     8581 cgtcaatttg ctgttgggct gttaactggt gtgaaggttg ataacctcga tggcgaagcg
     8641 gataccagta atgattattt gtcattactg cacggggaaa agcccatgat tacgcatgcg
     8701 gcagatttca aagaagaggc tgcaacgctc gtcaagcaaa ttcaggcatt gctggcgaat
     8761 caggttcgtt ctcaggatat ctgcattact gcgcgtacta aacatgcttg tgaccgctac
     8821 gcatcagcac tgaatgacgc cggaattgag acatttaatt taggcaatga tagcggtgac
     8881 agcgatgcac gccctggcgt gcgggttgca acgatgcacc gtattaaagg gcttgagttc
     8941 cagtatgtgt tcctggtggg gattaacgaa ggcgttgtgc cggaaataaa agcattagca
     9001 tccgatgatc ccgttgaaca gcgtgatgca ttatttaatg agcgcgcgtt actgcatgtg
     9061 gctgcaaccc gtgccgtaaa agggctgttt gtttcatcat ctggtgtccc tagtccgcta
     9121 ttagtggctg actgataata tgtccaaaac aggccaggag ccgaaacgct cctggtctat
     9181 ataaccgttc tggcaactgg cgatatttat attgcttagc tcccaacagc cagacaatat
     9241 caacttagaa attatattcg cttatgcggt ttgcatattg cacagtacaa cctgttttct
     9301 ctatatctaa cgtaacgtca ggatgtggat gatttttgga tggggtgaca cactggacaa
     9361 atatagctgg cacgaaaagg ctggcatttc cagctgacca atagtctctg gaaccatgat
     9421 gatgataaaa taaatatctt aagttttaaa cgtatgatgc caggattacc agaattataa
     9481 acgaatatat tagttctttt ctatttgttc aggaggggta taaaaattgc aattgttaat
     9541 agtatcgtca ggacgataaa aagataatca tgacaaaggc aattgaacta aaatgattga
     9601 cattttacag gtggtttgtc agtgatttct tgttattcat aatttatttt ccgta
//
LOCUS       DBFIFI010000167         9581 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.167, whole genome shotgun sequence.
ACCESSION   DBFIFI010000167 DBFIFI010000000
VERSION     DBFIFI010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9581
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.167"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..520)
                     /gene="mocA"
                     /locus_tag="N7B39_003731"
     CDS             complement(<1..520)
                     /gene="mocA"
                     /locus_tag="N7B39_003731"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HGU2423634.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIKRPNITNMRQALLMG
                     AHYSVEVENAEII"
     gene            623..1393
                     /gene="yqeC"
                     /locus_tag="N7B39_003732"
     CDS             623..1393
                     /gene="yqeC"
                     /locus_tag="N7B39_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HGU2423635.1"
                     /translation="MKSIVDPSALFIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANLGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRAMPLKAPDEHEPCIPKSSCCVIAVMGGHILGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            1441..3066
                     /gene="yqeB"
                     /locus_tag="N7B39_003733"
     CDS             1441..3066
                     /gene="yqeB"
                     /locus_tag="N7B39_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HGU2423636.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIIDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFA
                     AGKDCHAVIETNRGHWLGQVIYCGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(3107..4039)
                     /gene="arcC"
                     /locus_tag="N7B39_003734"
     CDS             complement(3107..4039)
                     /gene="arcC"
                     /locus_tag="N7B39_003734"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HGU2423637.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVVTLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVEAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(4087..5472)
                     /gene="hyuA"
                     /locus_tag="N7B39_003735"
     CDS             complement(4087..5472)
                     /gene="hyuA"
                     /locus_tag="N7B39_003735"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HGU2423638.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(5525..6736)
                     /gene="ygeY"
                     /locus_tag="N7B39_003736"
     CDS             complement(5525..6736)
                     /gene="ygeY"
                     /locus_tag="N7B39_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HGU2423639.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(6794..7990)
                     /gene="dpaL"
                     /locus_tag="N7B39_003737"
     CDS             complement(6794..7990)
                     /gene="dpaL"
                     /locus_tag="N7B39_003737"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HGU2423640.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(8048..9235)
                     /gene="ygeW"
                     /locus_tag="N7B39_003738"
     CDS             complement(8048..9235)
                     /gene="ygeW"
                     /locus_tag="N7B39_003738"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HGU2423641.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEQRTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
BASE COUNT         2227 a         2340 c         2513 g         2501 t
ORIGIN      
        1 aaattatttc tgcattttct acttcgacgg aataatgagc tcccataagg agtgcctgac
       61 gcatattggt gatattgggg cgcttgattg cctgcatcag gcatggtttt gacactaaaa
      121 tcggatggcc ggggatgcca ttatggagtg gcagtattgc gccatcattt cgtaacgacc
      181 agatttttct gaaaatatcg atggtgaggg ttggcatgtc accgtgggtg agaaaacaat
      241 gttctgtttg cactgcgggt actgcggcct ttactgatgt tagtaaaccc tgcgcataat
      301 ctgggttgtg gataatagtg atattgctct ggttcgcata acgttcgtgc agttcattac
      361 cacgatagcc ggtgactaaa ataattcggc tacaaaactg caacgcattt ttgatacttg
      421 tatcaagaat tgtcccctgt tgccagggta acatcatttt ccattgcccc attcttgatg
      481 ataatccggc agcggtaatt atacagtcga tggctgacat agtggttcca ttatttatag
      541 gggctaataa cgcctgataa ttgcgttact tcctgatgga ttaagttggg gataatttat
      601 cccagagagg tcataaagac tcgtgaaaag tatagttgac ccatcggcat tattcattga
      661 tttaggtgcg cagaaacgtc ccactgtgat ttctgttgtt ggtgctgggg ggaaaaccag
      721 cttgcttttt tggctggcag aactgctcca ggctagcggc aggcgtgtgt taatcacgac
      781 gacaacacat atgtttatgc caacgtctca ctggcccgtg gttttctgtc gtgatcccgc
      841 catgcttcct catgcgtctc ttacatcccc catttcattt tgttttcaca gctggaaagc
      901 gaacctggga aaagtgcagg gatttacgcc agaagcgatt gatgcactgg tgcaacgacc
      961 agagtgtgac gtaattctca ttgaagcaga tggctcgcgt gcaatgccgt taaaagcgcc
     1021 tgatgagcac gaaccttgca tacccaaaag cagttgctgc gtgattgctg tgatgggagg
     1081 acatattttg ggcgcgaaag tgagcacgga aaatgtccat cgctggtcgc agtttgctga
     1141 tattaccggg ttaacacctg atgcaacctt gcaactgagc gatctcgttg cgctggttcg
     1201 ccaccctcag ggggcgttta aaaacgtacc gcaaggttgt cggcgagtct ggttcattaa
     1261 ccgtttttct caatgtgaga atgcgattgc gcaaagcgag ctccttcaac cgctgcaaca
     1321 acacgacgta gaggcaatct ggctgggcga tatacaagaa catcctgcaa tcgcgcgcag
     1381 atttgtgaat tagcggcccg ggtattccgg gttactgaaa ggatatccat tgaggttggt
     1441 atgaatattt tcacagaggc tgcaaaactc gaagagcaaa attgtccgtt tgctatggcg
     1501 caaattattg atagccgagg ctcgactccc cgccattctg cacaaatgtt agtgcgcgcc
     1561 gatggctcta tcgtcggtac aattggtggc ggaatggttg agcggaaggt gattgaagag
     1621 tcgcttcagg cattgcagga acgtaagccg cgattattcc atggacgtat ggctcgtaac
     1681 ggtgcggatg ctgtcgggtc agattgtgga ggtgcgatgt cagtgtttat cagcgtccat
     1741 ggtatgcgtc cacgtctggt gttgattggc gcggggcatg tcaaccgggc gatagcccag
     1801 agtgcggcgc tattaggatt tgatattgcc gttgccgata tttatcgcga aagcctcaat
     1861 cctgaactat tccctccatc aaccacgctt ctccatgctg agtcgtttgg tgcggcagtg
     1921 gaagcactgg atattcgccc tgataatttt gtcctgattg ccacgaataa tcaggatcgt
     1981 gaagccctcg acaaactcat tgaacagccc attgcatggt tggggttgct ggcaagtcgt
     2041 cgcaaggttc agcttttcct gcgtcaattg cgtgagaaag gcgtggctga agaacatatt
     2101 gcccgtttac atgcgcccgt tggttacaac ataggtgcgg aaacgccgca ggagatcgcc
     2161 atcagcgtgc tggcagaaat attacaggtg aaaaataacg cgccgggtgg gctgatgatg
     2221 aaaccttctc atccttccgg acaccagctg gtggtgattc gcggtgcggg agatatcgcc
     2281 agtggtgtgg cgctacgtct gtatcatgcg ggttttaaag tgatcatgct ggaagtggaa
     2341 aaaccgacag tgattcgttg taccgtggcg tttgcccagg ccgtgttcga tggcgaaatg
     2401 acggtcgaag gcgtcactgc tcgcctggca accagctctg cggaagcgat gaaacttacc
     2461 gaacgcggat tcatccctgt gatggtagat cccgcctgtt cattgcttga tgaactgaaa
     2521 ccgctttgcg tggtggacgc tattctggcg aaacagaatt tgggaacacg ggcagatatg
     2581 gcaccagtaa caatcgcgct tgggccgggc tttgctgcag ggaaggattg tcatgcggta
     2641 attgaaacaa atcgcgggca ctggctcggt caggtgattt actgtggttg tgcgcaggag
     2701 aataccggtg ttcctggcaa tattatgggg cataccaccc gacgggtgat ccgtgctcct
     2761 gctgcaggca ttatgcgatc caacgtgaaa ttaggcgatc tggtgaaaga gggcgatgtg
     2821 attgcctgga ttggtgagca tgaaattaaa gcaccgttga cggggatggt gcgtggcttg
     2881 ttgaacgacg gcctggcagt ggttggtggt tttaaaattg gtgatatcga tcctcgtggt
     2941 gaaaccgctg atttcaccag cgtttctgat aaagcccggg cgattggcgg cggcgtactt
     3001 gaggcgttaa tgatgttgat gcatcagggc gtgaaagcga caaaagaagt gctggaagtg
     3061 gcttaaatga aaatggccgg atggtattac ccacccggcc ttaaaattac tgacaaatca
     3121 ccgttccggt tttgccttca atcccttctt tcgctttgct caatacggta atcagtgctt
     3181 cacgacctgc gcgagagcga gcaaacgagg cggcagcttc cactttaggc agcatagaac
     3241 ctttcgcaaa atgaccttct tcgataaagc gttcagcatc acttaatgac aggcgatcga
     3301 gccactgttc attctcttta ccgaagttaa tggccacttt ttctaccgca gtcagaatga
     3361 tcagcatatc ggcatcaatc atttctgcta aacgggcgct ggcccagtct ttatcgataa
     3421 ccgcgctggc accacgcaga tggttacctt cacgaataac aggaatacca ccaccgccaa
     3481 cggtgatcac cacctggcct gcttctacca gcgcttttac cgtctctttt tcaatgatat
     3541 caaccggttt tggcgaggca acaacacggc gatagccgcg accagcatct tctttcagag
     3601 tgtaaccctg ttttgtcagt tgctctgctt cttgctcggt aaagaacgaa ccgatcggct
     3661 tggtcgggtt gaggaatgcc ggatcgttag cgtcgacttc gacctgagta accagcgtta
     3721 ctacaggttt attaatgccc cgagaaagca gttcttctct taaggcgttt tgtagatcgt
     3781 aaccaatata accctggctt aaggcaacac agacagacat cggcagcatt ggcgaatgcg
     3841 cttcagtttt tgcggcagct tcaaaggcct ggttaatcat gcccacctgc ggcccgttac
     3901 catgagtgac gataacttca tgtccctggg caattaaatc aacaattgcc tgagaagtaa
     3961 tttttaccgc tttcatttgt ccggccaggt catcgcccag cgcattcccg cccagggcga
     4021 gaacaatttt cttactcatt tttcctcact catcattctt gattgtgtat agatccctgg
     4081 cagtggttag agcacgggag ggacaaacgg tttgcgtcgc aggaaacgac ctcgcccggc
     4141 tttgcctgta aaggtgccgt cacagaaaat cgtttcacca cgggataagg ttctgacaat
     4201 cgcgccctga caggtaaaac cctcccatgg cgagtagtcg gcgttgtcgt ggagatggcg
     4261 atgctgaatt tgttggctct gacgtgggtc gataatcacc acgtcgccat cggaaccggg
     4321 cgctaataat cctttttgcg gccacaggcc aaacagcctg gcgggcattg cgctggttaa
     4381 ttcaacaaag cgttccggtg ttatacgtcc cgtcatcacg ccactggaaa acagtaactg
     4441 catgcggttt tccacaccgg gtaagccatt tgggcagcga ctgaaatcgc ctttagaaat
     4501 ttgcaggcgt tgagccatcg agaaggtgca gtgatcggtc gccaccacgt caatcgcacc
     4561 atcgctgatg ccacaccaca gtttgtcctg ctcgcgtacg ttacgcagcg gtgggctaag
     4621 aatgaacttc atgccgtctt ctgtatcgta actgcgttcg tccaacagga gatattgtgg
     4681 gcaggtttca acccagactg gctggtgatt cgcacgggca agacgcagat aatccagacc
     4741 taagccgtta gacaggtgca cgatatagag cggggcgtta ccggcaattt gtgccaggtt
     4801 aatcatgcgg gcgatggctt ccgcttcgca ttccagaggg cgactcaagg catgatagcg
     4861 cggcgcggtt aaccctgcgg cgataaattc cgcccgcttg ctggcgatag ccgcatcatt
     4921 ttccgggtgc acggtggtca gcgcgccgga ttcatgcaga cggcgtaatg cctgcaaaac
     4981 ctcgtcatcg ttgagtttgt attgataggt taaatagagt ttaaaactgc tcagcccttc
     5041 ctcgaccatc atcgggattt cgtcgaggat ggcgtgatta atgtgctgga tcacaccgtg
     5101 aaagctgtaa tcgatgaccg ctttatgggc ggcataacca cgataaacct ccagttgatg
     5161 gcgtaaccga cagccgtttg ggccaaatcc catatggtca ataatggttg ttgtaccgcc
     5221 acacgcagct gcgcgggtac cggtaaaaaa atcatcacaa ctgcgcgcga tgccgacatc
     5281 aatattgaaa tgcgtatgga catccacgcc gccagggaaa acgtaacagc cagtggcatc
     5341 aatttcttca tacgggagct gcggcgaaat attgttgccc aactggcgaa caatcccgct
     5401 ttcaatcagc aaatcctgtt tggcttgtcc atctgcgtta acgacagtgc cgtttttgat
     5461 caatacgcgc atagcaaact ccaaaaaccc tccggcgaac cggaggggaa taagtgaaga
     5521 gtaattattc ggttgccagc cagcttaacg ggattgcagc gtacatcgcg gcacaggtca
     5581 ccaggtgaga tttccaggtt ttttcgttag gtgcatgcgc ttcaggttct ttacccgggc
     5641 caaagccgat caccggaatg ccgtgacggc ccatgataga tacgccgtta gttgagaagg
     5701 tccacttatc aacaaccggc gctttgccaa acagcccttc gtaggcattc accagtgctt
     5761 taacggtgaa gtgatcttct tccactttcc aggtcgggaa gtagcattcg gttgggtaaa
     5821 ccaggccagt ccaggacgga cggtcgtagt tgtacataga aacaaccgcg ttagctttct
     5881 gtactgcagg cagggcgcgg atttcgtcca gcgcgccttc ccaggtttcg ccccaggtca
     5941 gacggcggtc aatagagacg gcgcagctat ctgctacagc gcaacggctt ggggatgtga
     6001 agaagatttc agaaacggtg agggtgcctt tgccgaggaa ttcgtcataa cccagacgtt
     6061 gggagagttc ttgtaattcg ccaagaatcg gacccatttt gaaaatggcg ttgtcaccgc
     6121 gttctggcgc agaaccgtgg cagctaacac cctgaacatc aatacgaatt tccatacgac
     6181 cgcgctgacc acggtatacc tggcagtcgg ttggttcggt actgaccaca aattccgggc
     6241 gaatgccgga ttgttcaata atgtactgcc agcacagacc gtcgcagtct tcttcctgca
     6301 cagtaccggt aaccagcagg gtatattcat cttccagacc gaggtcttta atgattttac
     6361 cggcataaac catagatgcc atgccgcctt cctggtcgga agtaccgcga ccaccgatca
     6421 gctcatcagt ttccatgcct tcgtacggat cgaagtccca gtttttgatg ttgccaatgc
     6481 cgacggtatc gatatgagcg tccattgcca ccagacgcgg gccgtggccg atataaccga
     6541 gaacgttacc catcgggtcg atttcaactt tatcgaagcc gactttttcc atctcttctt
     6601 taatacgatg tactacccgt ttctcgtcgc agctttcact gggaatagca accatgtcgc
     6661 gcaggaagcg agtcatatcc gcctggtaat cttttgcttt ttcaagaatc agtttgaatg
     6721 gaatattctt agccattatt tttctccaga ttttttactc cccgttttgc agggagcagt
     6781 tccaataggg tgattaaggt gctacagcgt gtttgccttc ccagacaact tcgcggtagt
     6841 gcttcacgtc ggtgtcgcct tcagtgctga taaccagcac tacggcatct ttgttcagcg
     6901 ccagtttttc catcaggctt tgacgttgcg ggtgataatg aaccgctgcg agaacgccca
     6961 aaccgacagc gccggattca ccggagatga tgcgcgggtc gttgccgtac ggattaccca
     7021 gcacgcgcat acctaatgcg gcaacgctgt cctggcagga gatgaattgg gtggcgcagt
     7081 tacgtaggat ttcccagccc agcgggttag gttcgccaca ggccaggcct gccatgatgg
     7141 tggccatatc accgccaacg ttgacgatgt cgcctttgac gccggagcga taaatacagt
     7201 cagctttgtc aggttcaaca ataatgctgt gcagattttg cgggctatag acgtcgacca
     7261 gataacccag cacaccaccg gccattgctc cgacaccggc ttgcagcaga acgtgcgtcg
     7321 gggttacgcc catttcacgc atttgctcga cggcttcatc tgccagggtt gcgtagcctt
     7381 gcatgatcca ggttgggatt ttggtgtaac cttcccatgc cgtgtcctgt accacttccc
     7441 agccgtgctg ctgcgcgtgt tgcatggtca ggcgaacggt atcgtcatag ttcatatctg
     7501 tgacgatgca ctcggcaccg aggttcagaa tggcgtcaac gcgttcctga gcagaacctt
     7561 tcggcatgta aatcaccgca ttctgtccga gttgctgcgc tgcccacgcc acaccgcgcc
     7621 cgtggttgcc gtcggtggtg gtcgcgaaag tcattttttc gccgatggca tttttcaggt
     7681 gctcaaatga cagcgtttcg atatcaagat gatatttttc acacaataat tgagcgatag
     7741 cgtacgcacc gccgagcatt ttgaaggcgt tcagaccgaa tcgttttgat tcgtctttga
     7801 caagaatttt cttcacccca aaaaggtttg cgagatcgtc cagcgcacaa agcggtgttg
     7861 ggcgataacc agctattttc tggtggaact ggcgcgccag tttggcctgg ctttgcgaaa
     7921 agagcggtga tgtttcgccg ttgaaaaatt tgttatcggc gatatcaatc ttcaatgaga
     7981 aaacggacat atcctatcct tttggcctct ggaaatagag ggccgaccag ctgatcggcc
     8041 cgaggggtta tttcacgcgt tcttgcgctt ctttcagcag ttgctcgagc agtgcacctg
     8101 gtttggcgta tttacgggac aggatcatcg cggcgatgat ataaggcttc cagctggctt
     8161 ctttgtaggt agcgatacgg tatttttcga atacgccttc agtcacttcc ccttctttgc
     8221 aggatacgcc gctgatatca gctggcaggc agtgcatgta cagggcttcg ccatcacggg
     8281 tcagttccat catctcttca gtacaatgcc agtctttgtg ttgcgcgttc tgtgccagac
     8341 actgtttttc cagtgctttt aagccttcgt gatcgttagc gcgcagcaat tcggtacgct
     8401 gttccatcac tttgtaaggt gcccatgact tcggataaac gatgtctgca tctttgaacg
     8461 cttcttccat gctggtgacc tgacggaagc taccgccaga tgctttagca ttgtttttcg
     8521 cgacttctac tacatccggg atcaggtcgt agccttccgg atgggccagg gtgacatcca
     8581 taccgaagcg agtcatcaga ccgatgatgc cttgtggtac agagagcggt ttgccatagc
     8641 ttggagagta ggcccaggtc atggcgattt ttttaccttt caggttttcc agtgaaccaa
     8701 agtgttcacg taaccatgcc aggtcagcca ttgactgagt cgggtggtca atatcgcatt
     8761 gcaggttcac taaagccgga cgctgtggca gtacaccctg cttgtaaccg tcatcaagtg
     8821 cagcgccaac ttcacgcata taggcgttgc ctgcgcccag atacatatcg tcgcgaatac
     8881 caatagcgtc ggcgcagaag gagatcatat tggcggtttc acgcacggtt tcgccgtgag
     8941 cgatttgtga tttgccttca tcgagatctt gctgtgcaag gccgagcagg ttaagcgcgg
     9001 aagcataaga gaagcgggta cgagtggagt tgtcgcggaa tacggaaata cctaatccac
     9061 tattaaagac tttggttgag atgttttcag cacgcagtgc ttttaatgct gcggcaacgt
     9121 ccagtacttg tttcagttca tctggagtct gttcccacgt taacaaaaaa tctttctcgt
     9181 gaaggtgaga ggtcagcgaa ttgatatcct taatcagctc attaacagtt ttcatcataa
     9241 agtcccggta gtggaatgac aggcaaagtg gtttatttca ctgtgacttg ccaaagtgat
     9301 tggtgttttc acagctatac ctgggactaa caataagtgt gccagaatta tccacggggc
     9361 tgcaaaagac taatttgacg gataatgtgt ggggttgatc acaaattgat gccagcttcc
     9421 ggttgtttgt aaaatacgaa aacgtttggc tttttttatc gcgttattta tttgctgcta
     9481 agtatcagaa ataagataaa gggacaggct atccctcgtc agtttgataa actcaccgcc
     9541 ataattctca tattgatata gaaatgtgat atctcttcca t
//
LOCUS       DBFIFI010000168         9494 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.168, whole genome shotgun sequence.
ACCESSION   DBFIFI010000168 DBFIFI010000000
VERSION     DBFIFI010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9494
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.168"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            113..1498
                     /gene="dcuC"
                     /locus_tag="N7B39_003739"
     CDS             113..1498
                     /gene="dcuC"
                     /locus_tag="N7B39_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HGU2423642.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIIEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(1539..2219)
                     /gene="dpiA"
                     /locus_tag="N7B39_003740"
                     /gene_synonym="citB"
     CDS             complement(1539..2219)
                     /gene="dpiA"
                     /locus_tag="N7B39_003740"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HGU2423643.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2188..3846)
                     /gene="dpiB"
                     /locus_tag="N7B39_003741"
     CDS             complement(2188..3846)
                     /gene="dpiB"
                     /locus_tag="N7B39_003741"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HGU2423644.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISEVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            4226..5284
                     /gene="citC"
                     /locus_tag="N7B39_003742"
     CDS             4226..5284
                     /gene="citC"
                     /locus_tag="N7B39_003742"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HGU2423645.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIAMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            5299..5595
                     /gene="citD"
                     /locus_tag="N7B39_003743"
     CDS             5299..5595
                     /gene="citD"
                     /locus_tag="N7B39_003743"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HGU2423646.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            5592..6500
                     /gene="citE"
                     /locus_tag="N7B39_003744"
     CDS             5592..6500
                     /gene="citE"
                     /locus_tag="N7B39_003744"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HGU2423647.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            6511..8043
                     /gene="citF"
                     /locus_tag="N7B39_003745"
     CDS             6511..8043
                     /gene="citF"
                     /locus_tag="N7B39_003745"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HGU2423648.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLILASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTLFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            8047..8598
                     /gene="citX"
                     /locus_tag="N7B39_003746"
     CDS             8047..8598
                     /gene="citX"
                     /locus_tag="N7B39_003746"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HGU2423649.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            8573..9451
                     /gene="citG"
                     /locus_tag="N7B39_003747"
     CDS             8573..9451
                     /gene="citG"
                     /locus_tag="N7B39_003747"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HGU2423650.1"
                     /translation="MSMPATLTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQLLTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
BASE COUNT         2220 a         2558 c         2379 g         2337 t
ORIGIN      
        1 cagtttttct tgattttaat cagcattcat cgccaattta ttgggcatat tttttcctta
       61 agctttagga attttttatt atttactttg gggcctggag acaggaaaaa ttatgctgac
      121 attcattgaa ctccttattg gggttgtggt tattgtgggt gtagctcgct acatcattaa
      181 agggtattcc gccactggtg tgttatttgt cggtggcctg ttattgctga ttatcagtgc
      241 cattatgggg cacaaagtgt taccgtccag ccaggcttca acaggctaca gcgccacgga
      301 tatcattgaa tacgttaaaa tattactaat gagccgcggc ggcgacctcg gcatgatgat
      361 tatgatgctg tgtggatttg ccgcttacat gacccatatc ggcgcgaatg atatggtggt
      421 caagctggcg tcaaaaccat tgcagtatat taactcccct tacctgctga tgattgccgc
      481 ctattttgtc gcctgtctaa tgtctctggc cgtctcttcc gcaaccggtc tgggtgtttt
      541 gctgatggca accctatttc cggtgatggt aaacgttggt atcagtcgtg gcgcagctgc
      601 tgccatttgt gcctccccgg cggcgattat tctcgcaccg acttcagggg atgtggtgct
      661 ggcggcgcaa gcttccgaaa tgtcgctgat tgacttcgcc ttcaaaacga cgctgcctat
      721 ctcaattgct gcaattatcg gcatggcgat cgcccacttc ttctggcaac gttatctgga
      781 taaaaaagag cacatctctc atgaaatgtt agatgtcagt gaaatcacca ccactgcccc
      841 tgcgttttat gccattttgc cgttcacgcc gatcatcggt gtactgattt ttgacggtaa
      901 atggggtccg caattacaca tcatcactat tctggtgatt tgtatgctga ttgcctccat
      961 tctggagttc ctccgcagct ttaataccca gaaagttttc tctggtctgg aagtggctta
     1021 tcgcgggatg gcagatgcgt ttgctaacgt ggtgatgctg ctggttgccg ctggggtatt
     1081 cgctcagggg cttagcacca tcggctttat tcaaagtctg atttctatcg ctacctcgtt
     1141 tggttcggcg agtatcatcc tgatgctggt attggtgatt ctgacaatgc tggcggcagt
     1201 cacgaccggt tcaggcaatg cgccgtttta tgcgtttgtt gagatgatcc cgaaactggc
     1261 gcactcttcc ggcattaacc cggcgtattt gactatcccg atgctgcagg cgtcaaacct
     1321 gggccgtacc ctttcgcccg tttctggcgt agtcgttgcg gttgccggga tggcgaagat
     1381 ctcgccgttt gaagtcgtaa aacgcacctc ggtaccggtg cttgttggtt tggtgattgt
     1441 tatcgttgct acagagctga tggtgccagg aacggcagca gcggtcacag gcaagtaaat
     1501 agtaatgccg gaggcaagtt tcctccggct ttatcatgtc acccactgtg gtatatgcgt
     1561 tgcggtctgc caactttgcc gtgaacaatt tcagcaataa tcagatggcg gctggcgcaa
     1621 tattcaagat aacgcctggc agtggtgcga ctgatggtca gtgcctgcgc caccgtttct
     1681 gccgtatgtt gcacaccagg ctctttaaac agttttcgca ccgcgtttag cgtcaagggg
     1741 tcaatgccgg tcggcagttc gtctttgggt tcaccgcgag cataggcatt aaacatctca
     1801 tcaatttgct tctggctggc gctatcaata ctttccagca tatgtttacg ctggcggaaa
     1861 cgggttagcg tttgccccag ccgttcatag gcaatgggct taatgagata atcaaataca
     1921 ccacaacgta cggcttcaga caccgtttcc atatcgctgg ctgcagtggt aaacaccacg
     1981 tcgccgggat aatgcgcctg caccagttca tgcagtaaat taatccctct accgtcagga
     2041 agatagttat cgagcaagat tagccccggc ttaaaacgct cgatcatcat tcgggcctgc
     2101 gccaggtttc ccgccagtaa tatctgactg aatccgggaa tgtgacgaat atattcagca
     2161 tgcatctctg ccagcggcgt ttcgtcctca acgatcaata gggttaatgg agctgtcatt
     2221 tggtttcact ttcggaatat agattgaaaa taaggtaccg cagggatcat tatcttcgag
     2281 agtgataaca ccaccgcagc gcgttacgta gctggcaatc aagtacaacc caatgccatg
     2341 ttcaccgggc tcgtcagcac gcgtactgac cccctgctca aatattttgt ctcgtagaga
     2401 ctctggaacg ccgcagccct gatcggcgac ttcaatcacc acatcatcgc cttcatcgct
     2461 gaggaataat tcaacgatct tgtttccttc atcgctacgc aggctggctt cgaaggcgtt
     2521 atcaagtaaa ttgcccacaa tggctgcaaa ctcggtgcta tccagtcctg gcggcagttg
     2581 cgaaagctgg ctaccgggga caatgatcat ttttagcccc agttcccggg cgcgctgcac
     2641 tttaccaaaa agcagccccg ccacctggcg atcggcaaac gcctcgcgca ggctgtcaat
     2701 aagctgttgc tgggcctgag actccccctg caccatcgcc agcacgcgat catactcttt
     2761 catctgcaac agaccattga gcgtcgacat ccaattgaga tgctcgtgtc gcaatgtacg
     2821 aaggctctca acgtattgtt ttatttgcgt cagttgcgca ttgagggtgg aaatttcgtc
     2881 tttactgcga aagctgataa tggcccccag caaatcatca cctgaacgaa tagcttcccg
     2941 attggcaata acgctcagac cgttaaagtt agccaccaca tcctgacgtt tttcatcaat
     3001 ctgttcggta aagaaatcgg cgggcctgac cacttcagca atgggtttac ccaaccattg
     3061 ccgtccggga gagctcagcc ccagcatctt tcttgcgtta cgattgatgg cggtaatgta
     3121 accatgcgga tccaccgcaa tcagcccttc ataaaccgaa ctaaacagcg cctcttgctg
     3181 acggaccacg cgtgcgattt gctttggctc catgcccatc atttgccgac ggatatgcgc
     3241 tgccaggaac cacgacagca acatcagaat ccctaacagc acgacaaaca cgcctgccat
     3301 cggtaataaa aactcagccc gccagctatc gattttactc accaggtagc caatcgacac
     3361 cacgccgatg acttttccat cgtcatcaaa gattggcgtt ttggcgcgca tcgccatccc
     3421 cattgaccct ttcccggtaa tgaagtagct ctcccctttc tccagcgcgc cctgtttggt
     3481 gaactgcata ggataaccaa ttttctccgg attaggatgg taaaggcgga tcgagtgccg
     3541 gtccccaatc accacataat caaaatcggt atctctttgt aatttgttag cgatggtcgc
     3601 cagccgtttg tagtcacgcg ttttcacctc agagatgaca ctgtcattgg aggcaataat
     3661 tttcgcctga ttcattgcca tgtcgcgtac atgaagcgtt aaatagtcct caaaactggc
     3721 cgtaaaatat tgcgccaggg ctgcaatgac aaatattgag aacaccagaa tcagcaaaaa
     3781 gatacgcagc ggaaatgcca gtctttggaa aaatgcaaac tgtttattct cgttaagctg
     3841 caacattgtt ttccttgcgg gttgtcccga gcctctttga cggatgtaat taaaatgttg
     3901 aatcaaaata catagatatt aattatgatc gtggcaaatt ttattcatta aatcaattaa
     3961 atcacttaaa cctgcgagtc atttcgtaat tacattaatt aaaaaactta aaaccattaa
     4021 ataaataaaa aaccactaac tctatgtgaa ataaatcaaa atttcacgcc gaaatactcc
     4081 ttaggatgta tagcgaaagg agaaaaagat atacctcgat cacccccctt tctcccaagt
     4141 gaaaataaaa ggttatcagt ttgcaacatt gaacaacatt cgttgcaaat cgataacaac
     4201 atgcaccttc aggatactat ttattatgtt cggcaatgat attttcaccc gcgtaaaacg
     4261 ttcagaaaat aaaaaaatgg cggaaatcgc ccaattcctg catgaaaatg atttgagcgt
     4321 tgacaccaca gtcgaagtat ttattaccgt aacccgcgat gaaaagctta tcgcgtgcgg
     4381 tggaattgcc ggaaatatta ttaaatgcgt tgctatcagt gaatccgttc gcggtgaagg
     4441 actggcgctg acattagcca ctgaactgat aaacctcgcc tatgagcggc acagcacgca
     4501 tctgtttatt tataccaaaa ccgaatacga ggcgctgttc cgccagtgcg gtttttccac
     4561 gctgaccagc gtacccggcg tgatggtgct gatggaaaac agcgccacgc gactgaaacg
     4621 ctatgccgaa tcgctgaaaa aatttcgtca tccagggaac aagattggct gcattgcgat
     4681 gaacgccaat ccctttacga atggtcaccg ttatctgatt caacaagctg cggcacagtg
     4741 cgactggttg catctgtttt tagtcaaaga agattcttca cgcttcccct atgaagaccg
     4801 gctggatctg gtgttaaaag gcaccgccga tattccacgc ctgactgtgc atcgcggctc
     4861 cgaatacatc atctcccgcg ctacgttccc ttgctacttc attaaagaac agagcgtcat
     4921 taaccattgt tacaccgaaa ttgatctgaa gattttccgt cagtacctcg ctcccgcgct
     4981 gggtgtaact caccgctttg tcggtactga acccttttgt cgcgttaccg cccagtacaa
     5041 ccaggatatg cgctactggc tggaaacgcc gactatctcc gcagcgccca tcgaactggt
     5101 tgaaattgag cggctgcgtt accaggagat gccgatatcc gcttcccggg tacgtcaact
     5161 gctggcgaaa aacgatctca cggctatcgc gccgctggtc cctgcagtca cgctgcatta
     5221 tttgcagaac ctgcttgagc actcccgcca ggacgcggca gctcgtcaaa agacccccgc
     5281 atgagaaaca ggtgaaaaat gaaaataaac cagcccgccg ttgcaggcac ccttgagtct
     5341 ggggatgtga tgatacgcat cgccccactc gatacgcagg atatcgacct gcaaatcaat
     5401 agcagcgttg agaaacagtt tggcgatgca attcgcacca ccattctgga cgttctcgcc
     5461 cgctacaacg tgcgcggcgt acagctgaat gtcgatgata aaggcgcact ggactgcatt
     5521 ttacgtgcac gactggaagc cctgctggca cgcgccagcg gtatcccggc tctgccatgg
     5581 gaggattgcc aatgatttcc gcttcgctgc aacaacgtaa aactcgcacc cgccgcagca
     5641 tgttgtttgt gcctggtgcc aatgccgcga tggtcagcaa ctccttcatc tacccggctg
     5701 atgccctgat gtttgacctc gaagactccg tggcattacg tgaaaaagac accgcccgcc
     5761 gcatggttta tcacgcgctg caacatccgc tgtatcgcga tattgaaacc attgtgcgtg
     5821 taaacgcgct ggattccgaa tggggtgtta acgacctgga agccgtcgtt cgcggtggtg
     5881 cggacgttgt acgtctgccg aaaaccgata ccgctcagga tgttctggat atcgaaaaag
     5941 agatcctgcg tatagaaaaa gcctgtggtc gtgaacccgg cagcaccggc ctgctggcgg
     6001 cgattgaatc tccgctgggc attacccgcg cagtggaaat cgctcacgct tccgagcgtt
     6061 tgatcggtat cgccctcggt gcagaagact atgtgcgcaa cctgcgtaca gaacgctccc
     6121 cggaaggaac tgaactgctg ttcgcacgct gttccatttt gcaggccgcg cgctctgcgg
     6181 gtattcaggc gttcgatacc gtctattccg acgctaacaa cgaagccgga ttcctgcaag
     6241 aagccgccca catcaaacag ctgggctttg acggcaaatc gctgatcaac ccgcgtcaga
     6301 ttgatctgct gcacaacctc tacgcaccga cccagaaaga agtggatcac gcccgccgcg
     6361 tcgtagaagc cgctgaagcc gccgctcgcg aaggcctcgg cgtggtttcc ctgaacggca
     6421 agatggtgga cggtccggtt atcgatcgtg cccgtctggt gctctcccgt gcagaacttt
     6481 ccggcatccg cgaagaataa ggcaatcaaa atgacgcaga aaattgaaca atctcaacga
     6541 caagaacggg tagcggcctg gaatcgtcgc gctgaatgcg atcttgccgc tttccagaac
     6601 tcaccaaagc aaacctacca ggctgaaaaa gcgcgcgatc gcaaactgtg cgccaacctg
     6661 gaagaagcga ttcgtcgctc tggtttacag gacggcatga cggtttcctt ccatcacgct
     6721 ttccgtggcg gtgacctgac cgtcaatatg gtgatggacg tcatcgcgaa gatgggcttt
     6781 aaaaacctga tcctggcgtc cagctccctg agtgattgcc atgcgccgct ggtagaacac
     6841 attcgccagg gcgtggttac ccgcatttat acctccggcc tgcgtggtcc actggcggaa
     6901 gagatctccc gtggtctgct ggcggaaccg gtgcaaatcc actctcacgg cggacgtgtg
     6961 catctggtac agagcggcga actgaatatc gacgtggctt tcctcggcgt cccgtcctgt
     7021 gatgaattcg gtaatgccaa cggctacacc ggtaaagcct gctgcggctc cctcggctat
     7081 gcaatggttg atgccgacaa cgcaaaacag gttgtgatgc ttaccgaaga actgctgcct
     7141 tatccgcata atccggcaag cattgagcaa gatcaggttg atttaatcgt caaagttgat
     7201 cgcgttggcg atgctgcaaa aatcggcgct ggtgctaccc gtatgaccac taacccgcgc
     7261 gaactgctta ttgcccgtag cgctgcggat gtgattgtca actcaggcta cttcaaagaa
     7321 ggtttctcca tgcaaaccgg caccggcggc gcatcgctgg cggtaaccct tttcctggaa
     7381 gacaaaatgc gtagccgcga tattcgcgcc gacttcgccc tcggtggtat taccgcgacg
     7441 atggttgatc tgcacgaaaa aggtctgatc cgcaaactgc tggatgtaca gagctttgac
     7501 agccacgctg cgcaatcgct ggcccgtaac cccaatcaca tcgaaatcag cgccaaccag
     7561 tacgctaact ggggttcgaa aggtgcatcg gttgatcgtc tcgacgtggt ggtactgagc
     7621 gcgctggaaa ttgacaccca gttcaacgtt aacgtgctga ccggctctga cggcgtactg
     7681 cgtggtgctt ccggtggtca ctgcgatacc gcgattgcct ctgcgctttc catcatcgtc
     7741 gcgccgctgg tacgcggtcg tattccgact ctggtggata acgtgctgac ctgcatcacc
     7801 ccgggctcca gtgtcgatat tctggtcaca gaccacggta tcgcagttaa cccggcacgt
     7861 ccggaactgg cagaacgtct gcaggaagcg ggcattaaag tggtttccat tgagtggctg
     7921 cgcgaacgtg cgcgtctgct gaccggtgaa ccacagccga ttgaattcac cgaccgcgtc
     7981 gttgccgttg tgcgttaccg cgatggctcg gtgatcgatg ttgtgcatca ggtgaaggaa
     8041 taagccatgc acctgcttcc tgaactcgcc agccaccatg cggtgtcaat tcccgagctg
     8101 ctcgtcagcc gggatgaaag gcaagcacgg caacacgtct ggctcaagcg ccatcctgtt
     8161 ccactggtct cctttaccgt ggttgcgcct gggccgatta aagacagtga ggtcacacgc
     8221 cgaattttta atcatggcgt gacagccttg cgtgccttag ccgcaaaaca gggctggcaa
     8281 attcaggagc aggctgcact ggtttccgcc agcgggccgg agggtatgtt gagcattgcc
     8341 gccccggctc gcgacctcaa gctcgccacc attgagcttg aacatagtca tcctctcggg
     8401 cggttatggg atatcgatgt cctgacgccc gaaggcgaaa ttctctcccg ccgcgactat
     8461 tcactgccgc ctcgccgctg cctgttgtgc gagcaaagcg cagccgtctg cgcgcgtgga
     8521 aaaacccatc aactgaccga tttactcaac cgcatggagg cactgctgaa cgatgtcgat
     8581 gcctgcaacg ttaactaaaa ccacaaagct tgcgacgtca ttaatcgatg agtacgccct
     8641 gctgggctgg cgcgccatgc tgactgaagt caatctgtca ccgaaaccag gtctcgtgga
     8701 tcgcattaac tgcggtgcgc ataaagatat ggcgctggaa gatttccacc gcagcgcgct
     8761 ggcgattcag ggctggctac cccgtttcat tgaatttggt gcctgtagtg cggaaatggc
     8821 accagaagcg gtactccacg gattacgccc aattggtatg gcttgcgaag gtgatatgtt
     8881 ccgcgccacc gcgggcgtaa acacgcataa gggcagcatt ttttctttag ggctactgtg
     8941 tgcggcaatt ggccgtttgc ttcagctcaa ccagttgcta acgccaacaa ccgtttgttc
     9001 tacggcggca agtttctgcc gtggcctgac cgatcgcgaa ctgcgtacca ataattcaca
     9061 actgacggca ggtcaacggt tgtaccagca gcttggcctt accggcgcac gcggtgaagc
     9121 cgaagcgggt tatccactgg tgatcaacca cgccttgccg cattacctca ctctgctgga
     9181 tcaggggtta gatcctgaac tggcattgct cgataccttg ctcctactga tggcgatcaa
     9241 cggcgatacc aacgttgctt cgcgcggtgg cgaggggggc ctgcgctggc tacagcgcga
     9301 ggcgcaaaca ttattgcaaa aagggggcat tcgaaccccc gcagatctcg attatctccg
     9361 gcagttcgac agggagtgta tcgaacgaaa tctcagtcca ggcggcagtg ccgacctgct
     9421 gatccttacc tggtttttag cacagattta attatttaag aacttgataa atttggaaat
     9481 attaattttc ggag
//
LOCUS       DBFIFI010000169         9473 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.169, whole genome shotgun sequence.
ACCESSION   DBFIFI010000169 DBFIFI010000000
VERSION     DBFIFI010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.169"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..209)
                     /gene="cspA"
                     /locus_tag="N7B39_003748"
     CDS             complement(<1..209)
                     /gene="cspA"
                     /locus_tag="N7B39_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HGU2423651.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(490..780)
                     /locus_tag="N7B39_003749"
     CDS             complement(490..780)
                     /locus_tag="N7B39_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HGU2423652.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1214..1924
                     /locus_tag="N7B39_003750"
     CDS             1214..1924
                     /locus_tag="N7B39_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HGU2423653.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(1974..2948)
                     /gene="ghrB"
                     /locus_tag="N7B39_003751"
     CDS             complement(1974..2948)
                     /gene="ghrB"
                     /locus_tag="N7B39_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HGU2423654.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3052..3711)
                     /locus_tag="N7B39_003752"
     CDS             complement(3052..3711)
                     /locus_tag="N7B39_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HGU2423655.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            3864..6197
                     /gene="bisC"
                     /locus_tag="N7B39_003753"
     CDS             3864..6197
                     /gene="bisC"
                     /locus_tag="N7B39_003753"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HGU2423656.1"
                     /translation="MTNSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAEICGVNAEKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRAAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMDRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSELQWLETFYNVARQRGASQQVELPPFTEFWQANQL
                     IEMPENPDSERFIRFADFCRDPQAHPLKTASGKIEIFSQRIADYAYPDCPGHPMWLVP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGIQD
                     GDTVRLWNARGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6166..6606)
                     /locus_tag="N7B39_003754"
     CDS             complement(6166..6606)
                     /locus_tag="N7B39_003754"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HGU2423657.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKVNYWRECIPLVRDAY
                     LANAKNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYQAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(6603..7166)
                     /gene="tag"
                     /locus_tag="N7B39_003755"
     CDS             complement(6603..7166)
                     /gene="tag"
                     /locus_tag="N7B39_003755"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HGU2423658.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDRKKLFEMICLEGQQTGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7324..8022
                     /locus_tag="N7B39_003756"
     CDS             7324..8022
                     /locus_tag="N7B39_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HGU2423659.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALSQAENTTN
                     NSDYLYTMGVSARF"
     gene            8251..9453
                     /locus_tag="N7B39_003757"
     CDS             8251..9453
                     /locus_tag="N7B39_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2423660.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTIASGILLGLGFFLTAHSDNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVKFYVIFALLILSLALSTTIRQPEQ
                     KVLREAHI"
BASE COUNT         2224 a         2335 c         2466 g         2448 t
ORIGIN      
        1 aggctggtta cgttgccagc tgccgggcct ttagcgccgc tttcgatggt gaaggacact
       61 ttctgacctt cgtccagaga tttgtaacca tcgttctgga tagcagagaa gtgtacgaac
      121 acgtctttag agccatcgtc aggagtgatg aagccgaagc ctttgtcagc gttgaaccat
      181 tttacgatac cagtcatttt accggacata gtgtattacc tttaataatt aagtgtgcct
      241 ttcggcgata tggcgtgctt tacagatttt gaagcgttaa aggaatgtgc actacgaggg
      301 gtatcaacga taactcttga agggacttgc cttactacac tgctttaatg gtctgtacgt
      361 caaaccgttg atgtgcatta agccacgcat tggcgggtga tgcaacaatt atttttcata
      421 tttatgatta atcggttgga aaacggtcct gtcatcagga ccgtaaacag caataaagtg
      481 gataaaagtc tattccatca actgcttact taatgccggg ttggcttgaa tcaaacgcat
      541 caattttagt tcggcacttg aaggcttcac gcgtctggat tcccattcct ttaccatggc
      601 gactgatacg cccaaaaccc gggcgaaatc atcgattttt aatcctgtcc cttttcgtaa
      661 ctgctcaatt tcggtacagg acgttgttct gtgcgtcagg gtaattttct gcgtttcatc
      721 tttaaaaaca atctgttcca ggctgctcaa cagctcatgc attggatctt tatattccat
      781 tgaaaactcc tcaaatcaca ctgcgggatc gtgaattgca tcgaagctca ttaagaatag
      841 tcgggaaaag cagacacagc ggggtgaagt cagggcatgc tctggctatg attcgattca
      901 gccagtcatt tagcgtgact tatagccacc taacgatctg ataaatgccg atatatctct
      961 aaccactcct ggtttgtaaa gatttcgaaa atctaacctt ctgcgaaggc gtaaatctct
     1021 ggctacaaaa aaggcaacgg caatatcatt ttttaacaat gaaacaacaa attaaccttg
     1081 tttcattttc tccatgattt taccaggaag attacgtaga aagggtatcc tgcaagcctg
     1141 tcctggacta tccttgtcac gtcagacacg cgtgtgtcgt tgtgcgcttt ttttgggtga
     1201 aaggagtaag aaaatggcga caggaaagtc ctgctctcgc tggtttgcgc ctcttgcggc
     1261 gttattaatg gtagttagcc tgagtgggtg ttttgataaa gaaggcgatc agcgtaaagc
     1321 gtttatcgac ttcctgcaga atacagtgat gcgtagcggt gaacgtctac caaccctgac
     1381 tgccgatcag aaaaaacagt ttggtccttt tgtctctgat tacgcgattc tgtatggtta
     1441 ttctcagcag gtgaatcagg cgatggattc cggtctgcgc ccggttgtag acagcgttaa
     1501 cgcaattcgc gtaccgcagg attacgttac gcaaagcggt ccgctgcgtg aaatgaatgg
     1561 ttcgctgggt gtgctggcgc aacaactgca aaatgcgaag ttgcaggccg atgctgcgca
     1621 ctctgcgcta aaacagagtg atgacctgaa accggtcttt gatcaggcat tcaccaaagt
     1681 ggtaacgacg ccagctgatg cattgcaacc gttgattccg gcggcacaaa ctttcacgca
     1741 acaactggtg atggttggag actacattgc ccaacaaggt actcaggtga gctttgtagc
     1801 aaatggcatc cagttcccga cttcacagca ggcaagtgaa tataacaaac tgattgcgcc
     1861 attaccggca caacatcagg cgtttaatca ggcctggact acagcagtta ctgcaaccca
     1921 ataaagagta aaagcccgag cgattatcgg gctttactcc acgcagtcgc ggcttagtcc
     1981 gcgacgtgcg gattcacaca gttcttctca acctttcctt gtaacgcatc aatcaaatta
     2041 tccacggcac aggcggccat gccataacgc gtctcatggg tggcagatcc aatatgcggt
     2101 actgcgacga cgttggccat tgagagcaac ggcgaatcta cggaaagtgg ctcttgttcg
     2161 aagacatcca gcccggcggc gtgaatttcc cctttctgca atgctgcgat cagtgcattt
     2221 tcgtcaacca ccgggccacg tccggcatta atgaaaatgg cggaggattt cattttggcg
     2281 aattgttctg cgccaaacag atgatgcgtc tcatcagtta acggcaggat caggcaaacg
     2341 aaatctgact cttgtaacag tgtatccaaa tcgcagtagc gggcgttgaa gcgttcttct
     2401 gcttctttat ggtggcggcg cgcgttatag aggatgggca tgttgaagcc aaagtgcgca
     2461 cgttgtgcca gcgccatacc gatccgtccc atcccgacaa tgcccagtgt tttatggtga
     2521 acgtcagtgc cgtaccagtc cgggcctatg ctcgcggtcc attcgcctgc ttttacccgt
     2581 tctgctacct ccacaacccg acgagcggta gacaacacca gcgccatcag cgtatcggcg
     2641 acggtttctg ttaagacggt tggcgtgtgc atcagcagaa tttttcgggc ggtaagcgca
     2701 tcgacatcaa aattgtcata gccgacggag atcgttgatg tggcacgcag tttcggcatt
     2761 ttttccagca atgcggcatc aacattctcg tttgaaccca gtaaaccttc agcttcggca
     2821 aaaattgctg cattttgttc gacggtttgt gggctgaggt ttgccacctg gtgaacggtg
     2881 aaatgctctt gcaggcgttg cagtaaatca tcaggtaagg ctttgtagag gataacggac
     2941 ggcttcatgc ttctctccat tcactgataa ggcgatcacc ttagcctgaa atatgatgaa
     3001 ggaaaactga agaatgccag gtgatttctg catcacctgg catgaaaggg attacagcgg
     3061 gcttaaggta atttctacac ggcggttttg cgccttacct tctgcggtgc tgttgctggc
     3121 gattgggtta gccgggccaa ggccctgagt acggatgcgg ctggcgtcca cgccctgggt
     3181 gatcaacgcg ctggcaacgg aatccgcacg ctgctgggag agacgcatgt tcagatcgtg
     3241 accacccgtg ctgtcagtat aaccaatcac gttaaccgcc gttttcggat actctttcag
     3301 caccattgcc acgccggtca gggtgttagc gcccgccggt ttcagggttg cgctgctgct
     3361 gtcgaaggtc acattgttcg gcatattgag gataatgtta tccccgctgc gggttacgct
     3421 aacaccagtg ccgcgcattt tgtcacgcag cttcgcttcc tgcacatcca tgtaataacc
     3481 cacgccgccg cccagagctg cgcctgctgc tgcgccaatc agcgcgcctt taccgcgatc
     3541 tttcttcgaa gaagagagcg caccaatacc cgcgcccacg agagagccca gacctgcgcc
     3601 gatagcagat ttacctgctt cgcgttcgcc ggtgtaaggg ttagttgtgc agccagatac
     3661 cgccagagcg ccactcacta cggcggcaat aagataaaca cgtttcttca ttgttaatcc
     3721 ttaataacct ttttattctt tgccacgggt tccgtggcgg gagattatgc cgcgtgaaca
     3781 tgaagattat tcctgggaat actcggaaat ttgtaagtaa tatttaactg ctcaatacat
     3841 ctaacctttc aggagtcttc ggtttgacca actcatcctc acgatattcc gttctgactg
     3901 ccgcccattg ggggcccatg ctggttgaaa ccgacggcga aaccgtgttt agctcgcgtg
     3961 gcgcgttagc cacaggaatg gaaaactcct tgcagagcgc ggttcgtgac caggttcaca
     4021 gcaatacgcg ggtacgattt ccaatggtgc gcaaaggctt tctcgcgtca ccggaaaacc
     4081 cgcaaggcat tcgtgggcag gatgaatttg ttcgcgtgag ttgggatgag gcgctggatc
     4141 ttattcacca acaacataaa cgcattcgtg aggcttatgg tccggcatcg atttttgctg
     4201 gttcctacgg ctggcgttca aacggcgtgc tgcataaggc ctcgacatta ttacaacgct
     4261 atatggcgct ggcaggcggt tataccgggc atctggggga ttattcgacc ggcgcggcac
     4321 aggcgatcat gccgtatgtc gtgggtggta gtgaagttta tcaacagcag accagttggc
     4381 cgctggtgct ggaacatagc gatgtcgtgg tgctgtggag tgctaaccca ctcaatacgc
     4441 tgaaaattgc gtggaatgca tccgatgagc aggggctttc ttacttttct gcactgcgtg
     4501 acagcgggaa aaagctgatc tgcattgatc caatgcgatc ggaaaccgtc gatttctttg
     4561 gcgataaaat ggagtgggtg gcaccgcaca tgggcactga tgttgcgctg atgctgggga
     4621 tcgcccatac gctggtggaa aatggttggc acgacgaagc gtttctggcg cgttgcacca
     4681 ccggttatgc cgtcttcgcc tcttatttgc tgggcgagag tgacggaata gcgaaaaccg
     4741 ccgaatgggc agcagagatt tgtggtgtta acgcagagaa aatccgcgag ctggcggcta
     4801 ttttccacca aaataccacc atgctgatgg ctggctgggg aatgcagcgc caacagtttg
     4861 gtgagcaaaa acactggatg atcgtcacgc tggcagcaat gttggggcaa atcggcacac
     4921 ccggcggcgg ttttggtctt tcttaccatt ttgccaatgg tggtaacccc acgcggcgcg
     4981 ctgcggtact ctcttccatg cagggcagct tgccgggtgg ctgcgatgcg gtagataaaa
     5041 tccctgtcgc ccgcattgtt gaagcactgg aaaaccccgg tggcgcatat caacacaacg
     5101 gtatggaccg acatttcccg gatattcgtt ttatctggtg ggcgggcggt gccaacttta
     5161 ctcatcatca ggataccaat cgcctgatcc gtgcctggca aaaaccggag ctggtggtga
     5221 tctctgaatg cttctggacg gcggcggcaa aacacgcgga tatcgttctg cctgcgacta
     5281 cctcttttga gcgtaatgat ctcaccatga ccggtgatta cagtaatcag catctggtgc
     5341 cgatgaagca agtggtgccg ccacgctatg aagcgcgtaa tgattttgat gtttttgccg
     5401 agttaagtga acgctgggag aagggcggtt atgcacgttt tacggaagga aaaagtgagc
     5461 tgcaatggct ggaaacgttt tataacgttg cccggcagcg cggggcaagc cagcaggttg
     5521 aattgccgcc atttactgag ttctggcaag ccaaccagtt aattgagatg ccggaaaacc
     5581 cggacagcga gcggtttatt cgcttcgctg atttttgccg cgatccgcag gcgcatccat
     5641 taaaaaccgc cagcggtaag attgaaatct tctcgcagcg tattgccgat tacgcttatc
     5701 cggattgccc ggggcatcca atgtggctgg tgccggacga atggcagggc aatgccgaac
     5761 cggaacagtt acaggtactt tctgctcatc cggcgcatcg tctgcacagc cagctgaatt
     5821 acagttctct gcgcgaattg tacgcggtgg caaaccgtga gcctgtcacc attcatcctg
     5881 acgatgccca ggcgcgcggc atacaagatg gcgatactgt tcggctgtgg aacgcacgcg
     5941 ggcaaattct tgccggagcg gtcattagcg agggaattaa acctggcgtg atttgcattc
     6001 acgaaggggc atggccggat ctggatttaa ccgctgacgg tatttgtaaa aacggcgcag
     6061 tgaacgtgct gaccaaagat ctccccagct cgcggctggg gaatggctgt gcgggtaata
     6121 cggcgctggc atggctagaa aaatacaacg gtccggaact gacacttaca gcgtttgaac
     6181 caccggccag ctcataatcc aggtgggtag ttgggtttca tcctgccatg cgcaatcgac
     6241 aatgtgaaaa ccctgcgcct ggtaaaaatc tatcgccggt tgattttttt gataaacctc
     6301 cagcatcaga tggggatagc gctgctgcac atactgcatc agcgccttac caataccgcg
     6361 tctgacggcc ttcggtgcga caaacattgc tgccagaaat cggccttcca taatgctgac
     6421 aaaaccgaga agcttaccgt cttcttccca gacccagttt ttcgcattgg caagatacgc
     6481 atcccgcacc agcggaatgc actcgcgcca gtaattcact tttataaagg gatgccccca
     6541 ggttgtactt tccagccaca gttcgaggat cgcggggagt tctgaacgtt gcgcttcccg
     6601 aatcatggtt tatttcccgg atagcaacag cagccaacca catgatcatt caccagccca
     6661 catgcctgca taaaggagta acagattgtg gtaccgacaa acttaaaacc acgttttttc
     6721 agtgccttag atagggcgtc ggaggcgggc gtagatgtgg ggatttcgct caacgttgtg
     6781 gcttgtgtca cctgtggctg atgatttaca aacgaccaga caaagtcggc aaacggttcg
     6841 ccgttctgtt ccatttgcag gtacgcccgc gcattaccaa taattgcctg aattttcccg
     6901 cgatggcgga taatcccggc gtcctgtacc agtctttcga catcctcttc ctgcattgcg
     6961 gcaaccttca ccggatcgaa ctgatgaaag caggcgcgat agttttcgcg ttttttgagg
     7021 acggtgatcc acgataaccc agtctgctgc ccttccaggc agatcatttc gaacagtttt
     7081 ttcctgtcag tttcaggcac gccccactca ttatcatggt aggcaatata aagcgggtcc
     7141 tgactcaccc agccgcaacg ttccatactt tccctcgcta tgtacccgat cgtaaaaatt
     7201 tcagccgctt ttcttgacgc atcggtgaaa aagtcaatat ttaatacagg gctactatgc
     7261 ccgatatcct tcatacaatg acaataatat tgccgaattc ggctcttccc tggagtcgtg
     7321 ttaatgatca taaaaaaaag cggtggtcgc tggcagctaa gcctgctggc gagtgtggta
     7381 atcagtgcct tttttctcaa cacagcttac gcctggcaac aagaatatat cgttgatacg
     7441 caacccgggc attccacaga gcgttacacc tgggatagtg atcatcaacc tgattacaac
     7501 gatattttgt cgcaacgtat tcaaagtagc caaagggcgc tgggactgga agtcaatctg
     7561 gcggaagaaa cccctgtgga tgtgaccagc agtatgagta tgggctggaa ttttccttta
     7621 tatgaacagg ttacaactgg cccggtcgcg gcattacatt acgatggcac aaccacctcg
     7681 atgtataacg agtttggcga cagtactacc acgctgaccg atccgttatg gcatgccagc
     7741 gtgagtacct taggctggcg tgttgactcc cggcttggcg atctccgacc ctgggcgcaa
     7801 atcagctata accagcaatt tggcgagaat atctggaagg cgcaatcagg cctgagccgg
     7861 atgacggcga caaaccagaa cggcaactgg ctggatgtca ccgtaggcgc tgatatgttg
     7921 ctcaatcaaa atattgccgc ctatgccgcg ctatctcagg cagaaaatac cactaacaat
     7981 agcgattatc tgtatacgat gggggttagt gccagatttt aacgtaacaa tcacaattga
     8041 aaccattgaa taacaatagt tgtggcgata gtgggtgcta acttaccaaa taataaattt
     8101 ggtgaataat tgtcgcgtca ttcattcctg aactaaggca tttcattccg ttctgatggc
     8161 atttcatgcc gtttttcccc aggcataaag tgcacttcgt tatggttgtc ggcagagatt
     8221 tttccttttt attactgcag gaatactgcc atgacacctt caaattatca gcgtacccgc
     8281 tggttgacac tcatcggtac tatcattacc cagtttgcgc tggggtcggt ttatacctgg
     8341 agcctgttta acggcgcgct ttccgccaag ctggatgcgc cggtaagcca ggtcgctttc
     8401 tctttcggct tgttaagtct ggggctggca atatcgtctt ctgttgcggg caaattgcag
     8461 gaacgttttg gtgttaaacg cgtcaccatc gcttccggca ttttgctggg attagggttc
     8521 ttcctgacag cgcattctga caacctgatg atgctgtggt taagcgccgg tgtgctggtc
     8581 ggtctggcgg atggcgcggg ttacctgctg acgctctcta actgcgtgaa atggttcccg
     8641 gagcgtaaag gtctgatctc cgcgttcgct atcggttctt atggtctggg tagcctgggt
     8701 ttcaaattta tcgacacgca gctgctggaa acggtcggtc tggaaaaaac ctttgtgatt
     8761 tggggagcga ttgcgctggt gatgattgtt ttcggcgcaa cgttaatgaa agacgcacca
     8821 aaacaggaag tgaaaaccag caatggtgtg gtggagaaag attacacgct ggcagagtcg
     8881 atgcgtaaac cgcagtactg gatgttagcg gtaatgttcc tgactgcctg catgagcggc
     8941 ctgtacgtga ttggggtagc gaaagatatc gcccaaagtc tggcacacct tgatgtggtt
     9001 tctgcagcca atgcagtcac tgttatttcc atcgccaacc tttcaggtcg tctggtgctg
     9061 ggcattctgt ctgacaaaat cgcccgtatc cgtgttatca ccattggtca ggtaatttcg
     9121 ctggtgggta tggcggccct gctgtttgca ccattgaatg cagtgacgtt ctttgcagcg
     9181 attgcctgcg tggcatttaa ctttggcggc actattaccg tctttccgtc actggtcagt
     9241 gagttcttcg gcctcaataa cctggcgaaa aactacggtg tgatttacct cggtttcggt
     9301 atcggtagca tttgtggttc gattatcgcc tcactgtttg gcggcttcta tgtgaagttc
     9361 tacgtcatct ttgctctgct gattctgtca ttggcgcttt ctacgacgat tcgtcagcca
     9421 gagcagaaag tattgcgtga ggcgcatatt tagccagaaa ctacaggttt tga
//
LOCUS       DBFIFI010000170         9428 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.170, whole genome shotgun sequence.
ACCESSION   DBFIFI010000170 DBFIFI010000000
VERSION     DBFIFI010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.170"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            31..183
                     /gene="hokE"
                     /locus_tag="N7B39_003758"
     CDS             31..183
                     /gene="hokE"
                     /locus_tag="N7B39_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HGU2423661.1"
                     /translation="MLTKYALVAVIVLCLTVPGFTLLVGDSLCEFTVKERNIEFRAVL
                     AYEPKK"
     gene            534..686
                     /gene="hokE"
                     /locus_tag="N7B39_003759"
     CDS             534..686
                     /gene="hokE"
                     /locus_tag="N7B39_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HGU2423662.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(808..1428)
                     /gene="entD"
                     /locus_tag="N7B39_003760"
     CDS             complement(808..1428)
                     /gene="entD"
                     /locus_tag="N7B39_003760"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HGU2423663.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(1603..3843)
                     /gene="fepA"
                     /locus_tag="N7B39_003761"
     CDS             complement(1603..3843)
                     /gene="fepA"
                     /locus_tag="N7B39_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HGU2423664.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDNDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGITWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            4086..5288
                     /gene="fes"
                     /locus_tag="N7B39_003762"
     CDS             4086..5288
                     /gene="fes"
                     /locus_tag="N7B39_003762"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HGU2423665.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLISL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5291..5509
                     /gene="ybdZ"
                     /locus_tag="N7B39_003763"
     CDS             5291..5509
                     /gene="ybdZ"
                     /locus_tag="N7B39_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbtH family protein"
                     /protein_id="HGU2423666.1"
                     /translation="MAFSNPFDDPQGVFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            5506..9387
                     /gene="entF"
                     /locus_tag="N7B39_003764"
     CDS             5506..9387
                     /gene="entF"
                     /locus_tag="N7B39_003764"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HGU2423667.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSELPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEQTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLAGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAAFWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQHYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYQFSYREMREQVVALANLLREHGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     ADVPDLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFVPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMAPVVLLQLPQLPLSANGKLDRKALPLPELKAQTPGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPLWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFEKIGPIIRATLNR"
BASE COUNT         2070 a         2480 c         2590 g         2288 t
ORIGIN      
        1 gttccttctt cgcatggagg caatataaac atgctgacga aatatgccct tgtggcagtc
       61 atagtgctgt gtttgacggt accgggattt acgcttctgg tcggagactc gctgtgtgag
      121 tttacggtga aggaacgtaa tattgagttc agggctgttc tcgcttacga accgaagaag
      181 tagccgttgt gcggggagta atccccgcat atccggttgt caggtcagga tggtaaggca
      241 cctgctttac actttcgccc gcggtcagtg atggctgcgg gcgatttcgt tttaagagca
      301 atgatgagtt ttgccgacag aagtccgaaa caggattcac tgtataaata aacagctatt
      361 ttgctaagga agggtaagat aacggcgggt gcctgaagct ttccggtttc aggttaactc
      421 tgaggtctgg aaagatgaag ccccaggaga tatttctatc aatcctgggg ctgccactcc
      481 gagccccaac aactcggatg gtagtccctt cttcgcatgg aggcaatata aacatgctga
      541 cgaaatatgc ccttgtggca gtcatagtgc tgtgtttgac ggtgctggga tttacgcttc
      601 tggtcggaga ctcgctgtgt gagttcaccg taaaggaacg taatattgag tttaaggctg
      661 ttctcgctta cgaaccgaag aagtagccgt tgtgcgggga gcaatccccg catatccggt
      721 tgtcaggtca gagtggtaag gcacctgctt tacactttcg cccgcagtca gtgatggctg
      781 cgggcgaatc gtaccagatg ttgtcaatta atcgtgttgg cacagagtta tgactatttt
      841 ttcttttatc tgccagtgca cagcaaacat ctcattctca cgatgaatga tgacctgctg
      901 tttattccag ctaattatct gatagtccag aaaacctgca tcagtttgga tctcacttgc
      961 cttaaatgcg ctctctttgg cggaaaatgc cagtgtcagc gccagagaaa aggctaaacc
     1021 gcagtctgcg agtcgctcgt gttctgctgg tgtaataatg ttgtctgtca attcggtcgc
     1081 tgtttgcgca gaaaaaattt cttcgatatc aacgccaatc ggttgacggg aaaccacggc
     1141 caatgccgta gccccgcagt ggctaatact gccgtatacc tccgcaggcc acaccggttg
     1201 gcgtagctcg ccgattgcgg gcacacattt atagccatat tcccgcaagg cataaacagc
     1261 agcgatccgt ccggctaaat gctctgtttt acgtttacgt ccagcgtgtt gcagttgtgc
     1321 gtagtgcggc agccagagta aatcctgctc acaaaaattc gccggatcga actcaacaaa
     1381 atgcagcgta tgtccggcaa aggggaggga ggtatgcgta gttttcatat cgaccatatt
     1441 cgagactgat gacaaacgca aaactgcctg atgcgctacg cttatcaggc ctacatggtc
     1501 ctgcaatata ttgaattggc aagatttttg taggccggat aaggcgttca cgccgcatcc
     1561 ggcatgaacg acgcgcactt tgtcaacaat ctgacgttag catcagaagt gagtgtttac
     1621 gctcatatac cacgtacgtc ccggctcgtt ataggtatac gccccggcac cggcgatata
     1681 gttggcccct gccaaatcgc ccgtggtctg ggcattaccc gcacgccaca aacgtttgtc
     1741 gaacagattg tccacgccgc cggtcagact gacattcttc gtcacatccc acgtcgcgct
     1801 cagcccaaca atgctgtaag gactaatttc tttggtttcc ggtccaaccg ctggctgacc
     1861 tttatagttg tacttcttcg gctgctgctt accgtaccag gtgaaggtcg tttgcatcga
     1921 caaatcttct cgtgcctgcc agctcagcgt tgagttcaac gtatactccg ggatgatcga
     1981 caaacggtcg cccgtggttt tgttttcact cttcagcata taagtgatgt tattagtcca
     2041 catcaccgtt tcgctaaccg gtacgtttaa cgatccttcc agaccttcaa ccaccgcttt
     2101 cggcacgtta tcccactgat agagatcggt gccgactgcg ttttgcccta cagcaacata
     2161 gcctgcttca atcttattgc gataatcgtt acggaaccag gtgatgcccg ccagccagcc
     2221 gtcgcgtttg aactccagac caatctcttt gttgatgctg gtttctgctt tcaggtcatc
     2281 gttaccttgc agatagcagc cgcccgcgct ggcatagcag ccctgacctt tactgtagag
     2341 aatgtagttc gggttagtct ggtacaggct cggcgcttta taggcgcggg caatgcccat
     2401 tttcagcgtg aagtcatcgc ctaaaccttg cgatatgttc agcgccgggc tccagttatt
     2461 gccgacaata ctgtgatgat cgaaacgcag ccccggcgtt acgatggtgc tgtcagtcag
     2521 ctccatgttg ttttcggcaa acagcgagaa aatttctgct tttgaatacg ggctacggtc
     2581 ggtggcactc acgccatcaa tagcgccacc ggtattcgtt ccggtcagtg cctgggtgtt
     2641 ggaacttaag tccttcatcc gctgctgatt ccactccgta cccagcgtca gcgtctggtt
     2701 aacgaggaaa tcaatcggca ggttaacttc gctatgcagc atcacgtcat caagatcgtt
     2761 atcgacgaaa tcctgtgtcg ctttttcgtt aaatttccct tcggtaccgc ccgccagacc
     2821 ttccggaata cgcgagttac gggtgtgttc gtactgcacc cagttgctgg tggtcacgcc
     2881 gttatcccag ccaccgttcc aggtcagcga gtagttctgg cgatacaggc ggttggtttc
     2941 atcgccatat ttcgagcgag tgtaagcgtc agagttggtg ttctgggtat cgcccgcata
     3001 caggttaccc tggcggctgt aacccgcttc cagttccagc gattgcaatg gtgcgaagtc
     3061 ccagcgcacc acgccattaa tatctttatt gatcacccct tcgcgcccgg ctggtaacgt
     3121 cgtggcatac gttccggcac gcgcggactg atgaccctgg ttgatatccc acgcgtcagc
     3181 ctgggttttg tcgaggttgc catacaagcg gaagctgaat tcgtcgccca gcggaccggt
     3241 caggctaaag ttagtgcgtt tggtggcacc ttcctcttta tgttctggcg cattgaaata
     3301 tgcgtcccag gagccgtgcc actcgccgct gccttttttg gtaatgatgt taaccacgcc
     3361 gcccgccgcg ccgttgccat aacgcgcagc tgccggacca cgcagaactt caatacgttc
     3421 aatcatttca ggtggcaccc aggaggtatc accacgggta tcgcgctcgc cacgccagcc
     3481 ctgacgcacc gagttacggc tgcttaccgg cttgccgtca atcaaaatca gcgtgttttc
     3541 cggacccata ccgcgaatat caatctgtcg gttattccct cgctgaccac tggtggagtt
     3601 accggtcagg ttaacgcctg gcatggtacg gatgatctcc gaaacatcgc gggcaacggg
     3661 gtttttgcgg atttcatctg cggtgatggt cgaaacgcca ggcgcctgta agttctgctc
     3721 ggcggcggta acgacaatag tatcgtcatg tgaaacagga gtatcggtcg gctcttgtgc
     3781 ctgcgctacc ccataaatcc ccagattgac caacaaggcc agggaatgaa tcttcttgtt
     3841 cattgtttta ttcctgcatt tttgccacga attgcaactg tcgggcatgg tcgtcatcaa
     3901 cacgacgcat cccgctaccg cgaaaacctt tgatcctgaa agacacgcag tgcagttggt
     3961 taattaatgt ccgcgcttcc cacggcgcgc cattacgcta ttgcaaatgc aaatagttat
     4021 caataatatt atcaatatat ttctgcaatc aatgaaaaat tgcacagtaa acatggggtt
     4081 atggtgtgac ggcgttaaaa gtaggaagtg agagctggtg gcagtcgaaa catggcccgg
     4141 aatggcagcg tctgaatgac gaaatgtttg aggtcacttt ctggtggcgt gatccccaag
     4201 gttctgaaga atactcgacg ataaagcgcg tatggatcta catcactggt gtgaccgatc
     4261 atcatcagaa cagccagccc cagtcgatgc agcgaattgc aggtactgat gtctggcagt
     4321 ggacgacaca actcaatgcc aactggcgcg gcagctactg ctttattccc accgaacgcg
     4381 atgacatttt ttctgcacca tcccccgatc gcctcgaatt acgtgaaggc tggcgaaaac
     4441 tattacccca ggcaatagcc gatccgctga atccacaaag ctggaaaggc gggcgagggc
     4501 acgctgtttc tgcactcgaa atgccgcaag cgcctctgca accgggatgg gattgtccgc
     4561 aagcgccaga aataccagcc aaagaaatta tctggaaaag tgaacggttg aaaaattcac
     4621 gtcgtgtatg gatttttacc accggcgatg taacagcaga agaacgcccg ctggcagttt
     4681 tgctcgatgg cgaattttgg gcgcaaagta tgcccgtctg gcctgtgctg atttcgctga
     4741 cccatcgtca gcaacttcct cccgccgtgt atgtgttgat cgacgctatc gacaccacgc
     4801 accgcgccca cgaactgccg tgtaatgcgg atttctggct ggcagtacag caagagttat
     4861 tacccctggt gaaagctatt gcccctttta gcgatcgcgc cgatcgcacc gtggtcgccg
     4921 ggcagagttt tggtgggctg tccgcgctgt atgccggact gcactggcct gaacgctttg
     4981 gctgtgtatt aagccagtca ggatcgtact ggtggccgca tcggggcggg cagcaagagg
     5041 gcgtgttact tgaaaagcta aaagctggtg aagttagcgc cgaaggtctg cgcattgtgc
     5101 tggaagcggg tattcgcgag ccgatgatca tgcgggccaa tcaggcgctg tatgcgcaat
     5161 tacaccctat aaaagaatcc attttctggc gtcaggttga cggcggacat gatgcgcttt
     5221 gttggcgcgg tggcttgatg caggggttaa tcgacctctg gcaaccactt ttccatgaca
     5281 ggagttgaat atggcattca gtaatccctt cgatgatcca cagggagtgt tttacatttt
     5341 gcgcaatgcg caggggcaat tcagtctgtg gccgcaacaa tgcgccttac cggcaggctg
     5401 ggacattgtg tgtcagccgc agtcacaaga gtcctgccag cagtggctgg aagtccactg
     5461 gcgtactctg acaccggcga atttcaccca gttgcaggag gcacaatgag ccagcattta
     5521 cctttggtcg ccgcacagcc cggcatctgg atggcagaaa aactgtcaga attaccctcc
     5581 gcctggagcg tggcgcatta cgttgagtta accggagagg ttgatgcgcc attactggcc
     5641 cgcgcggtgg ttgccggatt agcgcaagca gatacgctgc ggatgcgttt tacggaagat
     5701 aacggcgaag tctggcaatg ggtcgatgat gcgctgatat tcgaactgcc agaaattatc
     5761 gacctgcgaa ccaatattga tccgcacggt actgcgcagg cattaatgca ggcggatttg
     5821 caacaagatt tgcgcgtcga tagcggtaaa ccactggtct ttcaccagct gatacaggtg
     5881 gcagataacc gctggtactg gtatcagcgt tatcaccatt tgctggtcga tggcttcagt
     5941 ttcccggcca ttactcggca gatcgccaac atttactgcg cattgctgcg tggcgaacaa
     6001 acgcctgctt cgccgtttac gcctttcgct gatgtagtgg aagagtacca gcaataccgc
     6061 gaaagcgaag cctggcagcg tgatgcggca ttctgggcgg aacagcgtcg tcaactgccg
     6121 ccgcccgcgt cactttctcc ggcaccttta gcggggcgca gcgcttcggc agatattcta
     6181 cgcctgaaac tggaatttac cgacggggaa ttccgccagc tggctacgca actttcaggt
     6241 gtgcagcgta ccgatttagc ccttgcgctg gcagcctttt ggctggggcg attgtgcaat
     6301 cgcatggact acgccgccgg atttatcttt atgcgtcgac tgggctcggc ggcgctgacg
     6361 gctaccggac ccgtgctcaa cgttttaccg ttgggtattc acattgcggc acaagaaacg
     6421 ctgccggaac tggcaacccg actggcagca caactgaaaa aaatgcgtcg tcatcaacgt
     6481 tacgatgccg aacaaattgt ccgtgacagc gggcgagcgg caggtgatga accgctgttt
     6541 ggtccggtac tcaatatcaa ggtatttgat taccaactgg atattcctgg tgttcaggcg
     6601 caaacccata ccctggcaac cggtccggtt aatgaccttg aactggccct gttcccggat
     6661 gaacacggtg atttgagtat tgagatcctc gccaataaac agcattacga tgagccaacg
     6721 ttaatccagc atgctgaacg cctgaaaatg ctgatcgccc aattcgctgc ggatccggct
     6781 ctgttgtgcg gcgatgttga tattatgctg ccaggtgagt atgcgcagct ggcgcagatc
     6841 aacgccactc aggttgagat tccagaaacc acgcttagcg cgctggtggc agaacaagcg
     6901 gcaaaaacac cggatgctcc ggcgctggca gatgcgcgtt accagttcag ctatcgggaa
     6961 atgcgcgagc aggtggtggc gctggcgaat ctgctgcgtg agcacggcgt taaaccaggg
     7021 gacagcgtgg cggtggcatt accgcgctcg gtctttttga ccctggcgct acatgcgatt
     7081 gttgaagcag gtgcggcctg gttaccgctg gataccggtt atccggacga tcgcctgaaa
     7141 atgatgctgg aagatgcgcg tccgtcgctg ttaatcacca ccgacgatca actgccgcgc
     7201 tttgccgatg ttccagattt aaccaacctt tgctataacg ccccgcttac accgcagggc
     7261 agtgcgccgc tgcaactttc acaaccgcat cacacggctt atatcatctt tacctctggc
     7321 tccaccggca ggccgaaagg ggtaatggtc gggcagacgg ctatcgttaa ccgcctgttg
     7381 tggatgcaaa atcattatcc acttacaggt gaagatgtcg ttgcccaaaa aacgccgtgc
     7441 agttttgatg tctcggtgtg ggagtttttc tggccgttta ttgccggggc taaactggtg
     7501 atggctgaac cggaagcgca ccgcgacccg ctcgctatgc agcaattctt tgccgaatat
     7561 ggcgtaacga ccacgcactt tgtgccgtcg atgctggcgg catttgttgc atcgctgacg
     7621 ccgcaaaccg ctcgccagaa ttgcgcgacg ttgaaacagg ttttctgtag tggtgaggcc
     7681 ttaccggctg atttatgccg cgaatggcaa cagttaacgg gcgcgccgtt gcataatcta
     7741 tatggcccga cggaagcggc ggtagatgtg agttggtatc cggcttttgg cgaggaactg
     7801 gcacaggtgc gcggcagcag tgtgccgatt ggttatccgg tgtggaatac gggcttgcgc
     7861 attctcgatg cgatgatgca tccggtgccg ccgggtgtgg cgggagatct ctatctcacc
     7921 ggtattcaac tggcgcaggg gtatcttgga cgacccgatc tgaccgccag ccgcttcatt
     7981 gccgatcctt ttgtccctgg tgaacggatg taccgtaccg gagacgttgc ccgctggctg
     8041 gataacggcg cggtggagta cctcgggcgc agtgacgatc agctaaaaat tcgcgggcag
     8101 cgtatcgaac tgggcgaaat cgatcgcgtg atgcaggcgc tgccggatgt cgaacaagcc
     8161 gttacccacg cctgtgtgat taaccaggcg gcagccaccg gtggtgatgc gcgtcagttg
     8221 gtgggctatc tggtgtcgca atcaggtctg ccgttggata ccagcgcatt acaggcacag
     8281 cttcgcgaaa cattgccgcc gcatatggcg ccggtcgttc tgctgcaact tccacagtta
     8341 cctcttagcg ccaacggcaa gctggatcgc aaagccttac cgttgcctga acttaaggca
     8401 caaacgccgg ggcgtgcgcc gaaagcgggc agtgaaacga ttatcgctgc ggcattcgcg
     8461 tcgttgctgg gttgtgacgt gcaggatgcc gatgctgatt tcttcgcgct tggcggtcat
     8521 tcgctactgg caatgaaact ggcagcgcag ttaagtcggc agtttgcccg tcaggtgacg
     8581 ccggggcagg tgatggtcgc gtcaaccgtc gccaaactgg caacgattat tgatggtgaa
     8641 gaggacagct cccggcgcat gggattcgaa accattctgc cgttgcgtga aggtaatggc
     8701 ccgacgctgt tttgtttcca tccggcatcc ggttttgcct ggcagtttag cgtgctctcg
     8761 cgttatctcg atccactatg gtcgattatc ggcattcagt cgccgcgccc tcatggcccc
     8821 atgcagacag cgacgaacct ggatgaagtc tgcgaagcgc atctggcaac gttacttgaa
     8881 caacaaccgc acggccctta ttacctgctg gggtattccc ttggcggtac actggcgcag
     8941 ggcattgcgg cgcgactacg tgcccgtggc gaacaggtgg catttcttgg cttgctggat
     9001 acctggccgc cagaaacgca aaactggcag gaaaaagaag ctaatggtct ggacccggaa
     9061 gtgctggcgg agattaaccg cgagcgcgag gccttcctgg cggcacagca gggaagtact
     9121 tcaacggagt tgtttaccac cattgaaggc aactacgctg atgctgtgcg cctgctgacg
     9181 actgctcata gcgtaccgtt tgacggaaaa gcgacgctgt ttgttgctga acgtacgctt
     9241 caggaaggta tgagccccga acgcgcctgg tcgccgtgga ttgcggagct ggatatctat
     9301 cgtcaggatt gtgcgcatgt ggatattatc tctccagggg catttgaaaa aattgggccg
     9361 attattcgcg caacgctaaa cagataaatt aacattatct ataaacccat aattgcagga
     9421 aataatta
//
LOCUS       DBFIFI010000171         9350 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.171, whole genome shotgun sequence.
ACCESSION   DBFIFI010000171 DBFIFI010000000
VERSION     DBFIFI010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9350
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.171"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            16..90
                     /gene="tnaC"
                     /locus_tag="N7B39_003765"
     CDS             16..90
                     /gene="tnaC"
                     /locus_tag="N7B39_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HGU2423668.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            311..1726
                     /gene="tnaA"
                     /locus_tag="N7B39_003766"
     CDS             311..1726
                     /gene="tnaA"
                     /locus_tag="N7B39_003766"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HGU2423669.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENASNIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1817..3064
                     /gene="tnaB"
                     /locus_tag="N7B39_003767"
     CDS             1817..3064
                     /gene="tnaB"
                     /locus_tag="N7B39_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HGU2423670.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFLVDYSILRDATSSTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3196..4371
                     /gene="mdtL"
                     /locus_tag="N7B39_003768"
     CDS             3196..4371
                     /gene="mdtL"
                     /locus_tag="N7B39_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HGU2423671.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIITSVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WTMAIMGIAVLMLSLFILKETRPAAPAASDKSRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLTVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGISAWNMLIGILIACSIVSLLLIMFVAPGRPVTAHEEIHHHA"
     gene            4346..5305
                     /gene="yidZ"
                     /locus_tag="N7B39_003769"
     CDS             4346..5305
                     /gene="yidZ"
                     /locus_tag="N7B39_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HGU2423672.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKQIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHEKWDLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRDTIKNLYASMA"
     gene            5462..6211
                     /gene="yieE"
                     /locus_tag="N7B39_003770"
     CDS             5462..6211
                     /gene="yieE"
                     /locus_tag="N7B39_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HGU2423673.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWVSKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWELDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTMKAFSPN"
     gene            6233..6799
                     /gene="yieF"
                     /locus_tag="N7B39_003771"
                     /gene_synonym="chrR"
     CDS             6233..6799
                     /gene="yieF"
                     /locus_tag="N7B39_003771"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HGU2423674.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(6853..8190)
                     /gene="adeP"
                     /locus_tag="N7B39_003772"
     CDS             complement(6853..8190)
                     /gene="adeP"
                     /locus_tag="N7B39_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HGU2423675.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRIRDLSPCVIIVALLFILKIVFIDAH"
     gene            8356..9021
                     /gene="yieH"
                     /locus_tag="N7B39_003773"
     CDS             8356..9021
                     /gene="yieH"
                     /locus_tag="N7B39_003773"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HGU2423676.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFREFGIHVDLKEIFT
                     RFKGVKLYEIIDIISKEQGVTMVQADAEHVYRAEVARLFDTELEDIAGANELLASINV
                     PMCVVSNGPVSKMQHSLGKLNMLHYFPEKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSSAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTELSQLPALWKARGWN
                     ITR"
     gene            complement(9158..>9350)
                     /locus_tag="N7B39_003774"
     CDS             complement(9158..>9350)
                     /locus_tag="N7B39_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2423677.1"
                     /translation="DEVNALNAKIVDCDDKIRSKLTIVRNNRLENQYKDDKNISDAMR
                     NILDWFERYPDIVQNITQA"
BASE COUNT         2304 a         2329 c         2320 g         2397 t
ORIGIN      
        1 gaggataagt gcattatgaa tatcttacat atatgtgtga cctcaaaatg gttcaatatt
       61 gacaacaaga ttgtcgatca ccgcccttga tttgcccttc tgtagccatc accagagcca
      121 aaccgattag attcaatgtg atctatttgt ttgctatatc ttaaatttgc cttttgcaaa
      181 ggccatctct cgtttattta cttgtgttag taaatgatgg tgcttgcata tatacatggc
      241 gaattaatgg atattgcaga tgcaatattc acagggatca ctgtaattaa aataaatgaa
      301 ggattatgta atggaaaact ttaaacatct ccctgaaccg ttccgcattc gtgttattga
      361 gccagtaaaa cgtaccactc gcgcttatcg tgaagaggca attattaaat ccggtatgaa
      421 cccgttcctg ctggatagcg aagatgtgtt tatcgattta ctgaccgaca gcggcaccgg
      481 ggcggtgacg cagagtatgc aggccgcgat gatgcgcggc gacgaagctt acagcggcag
      541 ccgcagctac tatgcgttag ccgagtcagt gaaaaatatc tttggttatc aatacaccat
      601 tccgactcac cagggccgtg gtgcagagca aatctatatt ccagtactga ttaaaaagcg
      661 cgagcaggaa aaaggcctgg atcgcagcaa aatggtggcg ttctctaact atttctttga
      721 taccacgcag ggccatagcc agattaacgg ctgtaccgtg cgtaacgtct atatcaaaga
      781 agccttcgat acgggcgtgc gttacgactt taaaggcaac tttgaccttg agggattaga
      841 acgcggtatt gaagaagttg gtccgaataa cgtgccgtat atcgttgcaa ccatcaccag
      901 taactccgca ggcggtcagc cggtttcact ggcaaactta aaagcgatgt acagcatcgc
      961 gaagaaatat gatattccgg tggtaatgga ctccgcgcgc tttgctgaaa acgcctattt
     1021 catcaagcag cgtgaagcag aatacaaaga ctggaccatc gagcagatca cccgcgaaac
     1081 ctacaaatat gccgatatgc tggcgatgtc cgccaagaaa gatgcgatgg tgccgatggg
     1141 cggcttgctg tgcatgaaag acgacagttt ctttgatgtg tacaccgagt gcagaaccct
     1201 ttgcgtagtt caggaaggct tcccgacgta tggcggcctg gaaggcggcg cgatggagcg
     1261 tctggccgta ggtctgtatg acggcatgaa tctggactgg ctggcttatc gtattgcgca
     1321 ggtgcagtat ctggtcgatg gtctggaaga gattggcgtt gtctgccagc aggcgggcgg
     1381 tcacgcggca ttcgttgatg ccggtaaact gcttccgcat atcccggcag atcagttccc
     1441 ggcacaggcg ctggcctgcg agctgtataa agtcgccggt atccgcgcgg tagaaattgg
     1501 ctctttcctg ttaggccgcg atccgaaaac cggtaaacaa ctgccatgcc cggctgaact
     1561 gctgcgttta accattccgc gcgcaacata tactcaaaca catatggact tcattattga
     1621 agccttcaaa catgtgaaag agaacgcgtc gaatattaaa gggttaacct ttacctacga
     1681 accaaaagta ttgcgtcact tcaccgcaaa actgaaagaa gtttaattaa tactactgag
     1741 tggctataag gatgttagcc actctcttac cctacatcct caataacaaa aatagccttc
     1801 ctctaaaggt ggcatcatga ctgatcaagc tgaaaaaaag cactctgcat tttggggtgt
     1861 tatggttata gcaggtacag taatcggtgg aggtatgttt gctttacctg tggatcttgc
     1921 cggtgcctgg tttttctggg gtgcctttat ccttatcatt gcctggtttt caatgcttca
     1981 ttccgggtta ttgttattag aagcaaattt aaattatccc gtcggctcca gttttaacac
     2041 catcaccaaa gatttaatcg gtaacacctg gaacattatc agcggtatta ccgttgcctt
     2101 cgttctctat atcctcactt atgcctatat ctctgctaat ggtgcgatca ttagtgaaac
     2161 gatatcaatg aatttgggtt atcacgctaa tccacgtatt gtcgggatct gcacagccat
     2221 tttcgttgcc agcgtattgt ggataagctc gttagccgcc agtcgtatta cctcattgtt
     2281 cctcgggctg aagattatct cctttgtgat cgtgtttggt tctttcttct tcctggtcga
     2341 ttactccatt ctgcgcgatg ccaccagctc cactgcggga acgtcttact tcccgtatat
     2401 ctttatggct ttgccggtgt gtctggcgtc atttggtttc cacggcaata ttcccagcct
     2461 gattatttgc tatggcaaac gcaaagataa gctaatcaaa agcgtggtct tcggttcgct
     2521 gctggcgctg gtgatttatc tcttctggct ctattgcacc atggggaata ttccgcgaga
     2581 aagctttaag gcgattattt cctcaggcgg caacgttgat tcactggtga aatcgttcct
     2641 cggcaccaaa cagcacggca ttatcgagtt ttgcctgctg gtgttctcca acttagctgt
     2701 cgccagctcg ttttttggtg tcacgctggg attgttcgat tatctggcgg acctgtttaa
     2761 gattgataac tcccacggcg ggcgtttcaa aaccgtgctg ttaaccttcc tgccacccgc
     2821 gttgttgtat ctgatcttcc cgaacggctt tatttacggg atcggcggtg ccggactgtg
     2881 cgccactatt tgggcggtca ttattcccgc agtgctggca atcaaagctc gcaaaaagtt
     2941 tcccaatcag atgttcacgg tctggggcgg caatcttatt ccggcgattg tcattctctt
     3001 tggtataacc gtaattttgt gctggttcgg caacgtcttt aacgtgttac ctaaatttgg
     3061 ctaaatcctt caagaagcca gtcattcgct ggcttcttgc ctctcaggaa atcacttatg
     3121 tccaaatggc aactcgcctg atcctccttc accacgtatg ctttgcgtca ccttactatc
     3181 aggacgcttt agcccatgtc ccgctttttg atttgtagtt ttgccctggt tttactttat
     3241 cccgctggga ttgatatgta cctcgttggt ttaccgcgca tcgccgccga tctcaatgcc
     3301 agcgaagcgc agttacatat tgcgttctcc gtttatctgg caggtatggc agctgcgatg
     3361 ttatttgccg gtaaagtggc cgatcgttca gggagaaaac cggtcgccat ccccggcgcg
     3421 gcgctattta ttatcacttc ggtgttctgt tcactggctg aaaccagcac gttatttctt
     3481 gcagggcgct ttctacaggg gttgggcgcg ggctgttgtt acgtggtggc attcgccatt
     3541 ttgcgcgaca cgctggatga tcgacgtcgg gcaaaagtgc tgtcgttact caacggcatt
     3601 acctgcatca ttccggtgtt agcgccggtg ctcggtcatc tgattatgct taaattcccg
     3661 tggcagagtc tgttctggac gatggcaata atgggcatcg cggtactgat gttgtctttg
     3721 tttattttaa aagaaacgcg ccctgccgcc cccgcagctt cggacaaatc acgagaaaat
     3781 agcgagtcgc tgcttaatcg gtttttcctc agccgtgttg ttatcaccac cctcagcgtt
     3841 tcggtgatcc tcaccttcgt caatacatcg ccggtattgc tgatggaaat catgggtttt
     3901 gagcgcggag aatacgccac cattatggcg ttgaccgctg gcgtcagcat gaccgtttca
     3961 ttttccacgc catttgcgct gggaattttt aagccacgta cgttgatgat cacctcacag
     4021 gtgttattcc tggcggcggg catcactctt accgtttcac cttcccatgc ggtttctctg
     4081 tttggtatca cgctgatttg cgccggtttc tcggtaggtt ttggtgtggc gatgagtcag
     4141 gcgttagggc cgttttcatt acgcgcgggc gtagccagct cgaccttagg tattgcgcag
     4201 gtttgcggtt cgtcactgtg gatttggctg gcagcggtgg ttggtatcag cgcatggaat
     4261 atgctgatcg ggattctgat tgcctgtagc atagtgagcc tgttgctgat tatgttcgtc
     4321 gcgcctggac gcccggttac cgctcatgaa gaaatccatc accacgcttg atctcaatct
     4381 gctgctctgt ctgcagctgc tgatgcagga gcgcagcgta accaaagcgg cgaagcggat
     4441 gaacgtgaca ccttcggcgg tgagtaagtc gctggcaaag ttaagagcgt ggtttgacga
     4501 cccgctcttt gtgaactcac cgttggggct gtcgcccaca ccgctaatgg tcagcatgga
     4561 gcaaaatctg gcggagtgga tgcaaatgag caacctgctg ctggataaac cgcaccacca
     4621 gacaccgcgc ggcctgaagt ttgagctggc ggcagaatca ccgctaatga tgatcatgct
     4681 taatgcgctg tcgaaacaga tctaccaacg ttacccgcag gcgaccatca aattacgtaa
     4741 ctgggattac gattccttag atgccattac tcgtggtgaa gtggatatcg gtttttccgg
     4801 tcgcgaaagt catccccgtt cgcgggagct gttaagctcg ctaccgttag ccattgatta
     4861 tgaagtgttg tttagtgatg tgccctgcgt ctggttacgc aaagatcatc cggcactgca
     4921 tgaaaagtgg gatctcgata cctttttacg ttatccgcat atcagcattt gctgggaaca
     4981 gagcgatacc tgggcgctgg acaatgtgtt acaggagctg gggcgcgaac gtacgattgc
     5041 aatgagcctg ccggaattcg agcagtcact gtttatggca gcgcagcccg acaatctgct
     5101 cctggcgacc gcgccgcgct actgtcagta ctacaatcaa ctccaccaac tgccgttggt
     5161 tgctcttcct ctcccgtttg acgaaagcca gcaaaaaaag ctggaagtcc cttttaccct
     5221 gctgtggcat aaacggaaca gccataatcc gaagatcgtc tggttacggg ataccattaa
     5281 aaatctttac gcgtcgatgg cataaccgaa tcgtatgaaa cgcgacccga tttcacaata
     5341 aaatgtaaaa aagttgtaat aagcttgtct gaatcgaact tttagccgct ttagtctgtc
     5401 catcattcca gtaaatgatt actcttgtat tcataatgga ccattaagca tggagcgaaa
     5461 aatggcgact cactttgccc gagggatttt aacggaagga catctgattt ctgttcgtct
     5521 cccctcccag tgtcatcaag aagcccgaaa cattccccct catcgtcaaa gccgttttct
     5581 ggcgtccaga ggtttactcg cagaactgat gttcatgctg tatggcattg gcgaattgcc
     5641 ggaaatcgtc accctgccga aaggtaaacc ggttttcagt gataaaaatt tgccttcgtt
     5701 ttctatttcc tatgccggga atatggttgg cgtggcgtta acaaccgaag gtgaatgtgg
     5761 cctcgatatg gaactgcagc gtgcgacgcg cgggtttcat agcccacacg cgcccgataa
     5821 ccacacattt tccagcaatg aatcgctatg ggtcagtaaa caaaatgatc caaacgaagc
     5881 gcgggcgcag ctcatcacgc tgcgccgaag cgtgctaaaa ctaaccggtg atgttttgaa
     5941 tgacgatccg cgcgatctgc aactgctgcc cattgccgga cgcctgaaat gtgctcatgt
     6001 aaatcatgta gaagcgttat gcgacgcgga ggacgtgctg gtgtggtccg tggcggtcac
     6061 gcccacgatt gaaaagctca gtgtctggga gttagatggc aaacacggct ggaaaagcct
     6121 gccggatatt cacagccgcg ccaacaatcc taccagccgg atgatgcgtt ttgcccagct
     6181 ctctaccatg aaggcttttt cgccaaattg atagacaacc acaggagtca ttatgtctga
     6241 aaaattgcag gtggttacgt tactggggag cctgcgcaaa ggctcattta atggcatggt
     6301 tgcacgtacc ctaccgaaaa ttgctccggc gagcatggaa gtcaatgcgt taccttccat
     6361 tgccgacatt cccctgtatg acgctgacgt acagcaggaa gaaggttttc cagcaacggt
     6421 tgaagctctg gcggaacaga tccgtcaggc tgacggtgtg gtgatcgtca cgccggaata
     6481 taactattcg gtaccgggtg ggctgaaaaa tgccatcgac tggctttccc gcctgccgga
     6541 tcaaccgctg gccggtaaac cggtgttgat tcagaccagc tcaatgggcg tgattggcgg
     6601 cgcgcgctgt cagtatcacc tgcgccagat tctggttttc ctcgatgcaa tggtgatgaa
     6661 caagccggaa tttatgggcg gcgtgattca gaacaaagtt gatccgcaaa ccggagaagt
     6721 gattgatcag ggtacgctgg accacctgac cgggcaattg accgcatttg gcgagtttat
     6781 tcagcgagtt aagatctaaa taaaaaaccc gccagcaatc atgcatggcg ggtttttaac
     6841 gcgctatcga ttttagtgag cgtcgataaa tacaatcttc aggataaaca gcagcgcaac
     6901 gatgattacg cacgggctaa ggtcacgaat acgcccggta ccaatcttca tcacgcagta
     6961 ggagataaag cccagcgcaa taccttcggt aatcgagaag ctgaacggca tcatcacagc
     7021 ggtaataaac gccggaacag attcagtaag atcctgccag ttcacgcgag caagacttga
     7081 ggtcatcaac acgccaacgt aaatcagcgc gccagctgca gcgtagcctg gcaccattcc
     7141 cgccagcggc gacagaaaga taaccagcag gaacagcaga ccaacaacca ctgccgtcag
     7201 accggtacga ccgccaaccg atacgccgga agaggactca atataagccg taacggaaga
     7261 agtaccgata aacgaaccgg tcacggaaga gatactgtcg acatacagcg cctgcttcat
     7321 gcgcgggaat ttccccttct catctgccag accggcttta tcggtcacgc caatcaacgt
     7381 gccggaggag tcaaacaggt tgaccagcat gaaagagaaa atcacccctg ccagcccgag
     7441 gttaaacgac ccggctaaat ctacatgacc cacgacagtc attacgctcg gcggcgcaga
     7501 aacgatgcca ttgtagtgga catcacccag catccagccc agcagcgttg tcaccacgat
     7561 agaaaccagc accgctgcgt gaatgttgcg tgaggccaga atagcaatga tgaagaagcc
     7621 gaggatcccc agcagcacgc tgtgagaagt cagattaccg atgctgacca gcgtttccgg
     7681 gttagcgaca atcacacctg cgtttttcag ccccatcatg ccaatgaaca gaccgatacc
     7741 gctggtaatg cccacacgca gactcaccgg aatgttggca atcatccagt agcgaacgcg
     7801 gaaaatcgtc agtaacagca gaccaatcgc gccccagaag attgcgccca tcccgacttg
     7861 ccacggcaag cccatcgcct gcacaacgac aaaagcgaag aacgcattca ggcccatagc
     7921 gggtgccagt gcaactggca ggttagcaaa cagccccatc ataatactgc cgaatgcagc
     7981 aatcagacag gtagtgacga agacggcgct ggtatccatg ccagcaacgc caagaatttg
     8041 cgggttaaca aaaacgatgt aaaccatcgt caggaaggtg gtaaaaccgg cgatcacttc
     8101 ggtccgtgcc gtcgtgccat gttcgcgcag tttaaacacg cgttccagca tcccctggcc
     8161 agaagcctgg gttgtgtgtt gttgactcat tatctatttc cgaacaagga gggaaaatcc
     8221 gtcgctatcg tataccaaaa tgagacaata tggacggctg aagataactt ttttcattgt
     8281 atttgcttat acggcaacga ttgcgtcatg cgaattggca tgacgaaaac gttaaactaa
     8341 cgataaagga aagacatgtc ccagatagaa gcggtatttt tcgactgcga cggtacgctg
     8401 gtcgatagcg aggtcatttg ctctcgcgcc tatgtcacga tgttcaggga atttggcatt
     8461 cacgttgacc tgaaagagat ttttacccgt tttaaaggcg taaaactcta cgaaatcatc
     8521 gatatcatca gtaaagaaca gggcgtgacg atggtccagg ccgatgccga gcacgtttac
     8581 cgcgcagaag tagcacgtct gtttgatacc gaacttgagg acatcgcagg agcgaacgaa
     8641 ttgctggcga gcattaacgt gccgatgtgc gtcgtctcta acggtccggt cagcaaaatg
     8701 cagcattcgc ttggtaagct caacatgctg cactacttcc cggaaaaact gttcagcgga
     8761 tatgacattc agcgttggaa gcccgatccg gcattaatgt tccatgcggc aaaagcgatg
     8821 aatgtgaacg ttgaaaactg cattctggtc gatgactcca gcgctggcgc tcaatcaggg
     8881 atagatgccg gaatggaagt gttttacttc tgcgctgacc cgcacaataa gccgatcgtt
     8941 cacccgaaag tcaccacctt taccgagctg tcgcagctgc ctgcgctctg gaaggcacgc
     9001 ggctggaata taacgcgata atcgcctcct tacttcaaca cttacacatc agtcacgctg
     9061 gtttgagata cagaacacca ggtgtctgat gagtaagcgt ctattcccta tgaataattt
     9121 atgtcgttat tcgtcctgtc atccaccctc tgtatggtta agcctgggtt atattttgta
     9181 caatatcggg ataccgttcg aaccaatcca gaatattacg catcgcgtca ctgatgtttt
     9241 tatcgtcttt atattgattc tcaagtctat tatttcgaac aatagtaagc ttagaacgta
     9301 ttttatcatc acaatcgaca attttcgcat tcaatgcatt tacttcatcc
//
LOCUS       DBFIFI010000172         9254 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.172, whole genome shotgun sequence.
ACCESSION   DBFIFI010000172 DBFIFI010000000
VERSION     DBFIFI010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9254
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.172"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..87)
                     /locus_tag="N7B39_003775"
     CDS             complement(<1..87)
                     /locus_tag="N7B39_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU2423678.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNI"
     gene            271..1323
                     /gene="ybhH"
                     /locus_tag="N7B39_003776"
     CDS             271..1323
                     /gene="ybhH"
                     /locus_tag="N7B39_003776"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HGU2423679.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEKRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGGLDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            1399..2832
                     /gene="ybhI"
                     /locus_tag="N7B39_003777"
     CDS             1399..2832
                     /gene="ybhI"
                     /locus_tag="N7B39_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HGU2423680.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESSVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            3015..5276
                     /gene="ybhJ"
                     /locus_tag="N7B39_003778"
     CDS             3015..5276
                     /gene="ybhJ"
                     /locus_tag="N7B39_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HGU2423681.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVAALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGAAQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGSSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     VEAPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(5417..6700)
                     /gene="ybhC"
                     /locus_tag="N7B39_003779"
     CDS             complement(5417..6700)
                     /gene="ybhC"
                     /locus_tag="N7B39_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HGU2423682.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWGDAVISNRPFAGNTGSVD
                     DSDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(6835..7905)
                     /locus_tag="N7B39_003780"
     CDS             complement(6835..7905)
                     /locus_tag="N7B39_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU2423683.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMS
                     KIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHI
                     TTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCE
                     MKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTR
                     REPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHK
                     SDTMASQYRDDRGREWDKIEIK"
     gene            complement(7883..8101)
                     /locus_tag="N7B39_003781"
     CDS             complement(7883..8101)
                     /locus_tag="N7B39_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HGU2423684.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTGSLLKRIRNGKKAKS"
     gene            complement(8141..8308)
                     /locus_tag="N7B39_003782"
     CDS             complement(8141..8308)
                     /locus_tag="N7B39_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2423685.1"
                     /translation="MHFRVTGEWNGEPFNRVIEAENINDCYDHWMIWAQIAHADITNI
                     RIEELKEHQAA"
     gene            8551..9153
                     /locus_tag="N7B39_003783"
     CDS             8551..9153
                     /locus_tag="N7B39_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2423686.1"
                     /translation="MSYFLRKKWMVNLSGAGKILWALNMKKDSYPYLICMTVLGLIFI
                     FLFFWWRADIYRVTFLNQSISHYYILFSMGIAFLLSLFWVKKGIVKQSGWKSLSAYLK
                     VYAGMCIFAGFFLIIPLTTLTYFLPGETSSYVAPYRYTSGSSKSCSGAEVDDPDLHEN
                     IRICYPYGNYEYDNIIYVEKKINTLGAVVTYAQTARDDTE"
BASE COUNT         2241 a         2135 c         2294 g         2584 t
ORIGIN      
        1 aatattgagt aatttagcgg cattgttaaa tgaactggac tctgccagta tgacaaatgc
       61 cttcatactt gataattcat gcttcattat tactccggga aatggaagcg acgattttgg
      121 gtggctggcc gttaaaaatt ttaactgcat ttagccaact taaattaatg aaaaaatgtt
      181 attaatcgtt gagctaaagt cattagagat gctttgccct taatgtaacc atatcgcaat
      241 aagttatgtt tttaaattga gggcattatt atgaaaaaaa taccctgcgt gatgatgcga
      301 ggtggaacct cgaggggcgc gttcctgtta gcggaacatt tacccgaaga tcaaacgcag
      361 cgcgataaaa tattgatggc aattatgggt tccggtaacg atctggaaat tgacggtatt
      421 ggcggcggta atccactgac cagtaaagtc gccattatta gccgttccag cgatccgcgt
      481 gctgatgttg attatctgtt tgctcaagtt atcgtccatg agaaacgtgt cgataccacg
      541 cctaactgcg gcaatatgct gtctggcgtt ggggcattcg ccattgaaaa tggtttgatt
      601 gcagcgactt cgccagttac ccgcgtacgt atccgcaacg tcaatacggg tacgttcatc
      661 gaagctgatg tgcaaacgcc aaatggtgtt gtcgagtacg agggtagcgc cagaattgac
      721 ggcgtaccgg gtactgccgc accggttgcg ctcactttcc tgaatgccgc tggaacgaaa
      781 accggaaaag ttttcccgac tgataatcag attgattatt ttgacgatgt cccggtgacc
      841 tgtatcgata tggcgatgcc agtcgtcatt attccggctg aatatctggg aaaaacaggc
      901 tatgaattac cggcggaact ggatgccgac aaagcattat tagcccgcat tgaatctatc
      961 cgtctacaag cgggtaaagc aatgggctta ggcgatgtca gtaatatggt tatccctaaa
     1021 cctgtgctta tttctccagc gcagaaaggc ggggcaatta atgtgcgtta ttttatgccg
     1081 cattcttgcc atcgcgcgct ggcgataacc ggtgctattg ctatttccag tagttgtgca
     1141 ttggaaggta ccgtcacccg acaaatcgtc ccttctgtag gatacggcaa tatcaatatt
     1201 gaacacccca gtggtgggct cgacgttcat ttaagtaatg aaggtcagga tgccacgacg
     1261 ttacgcgcat ctgttattcg gacaaccaga aaaatatttt ccggtgaagt ttatcttccc
     1321 tgaaaagatt cgttgtcagg ataaggacaa tcaataaagg acttctgtat gagtcataca
     1381 gaaagaacag gattttaaat gaataagaaa tcgttatgga agctaattct gatattagcg
     1441 atcccatgta ttattggctt tatgccagct ccggcaggat taagcgaact ggcgtgggtg
     1501 ctttttggta tttacctggc ggccattgtg gggctggtta tcaagccttt cccggaacct
     1561 gtcgtactgt taattgccgt tgctgcctcc atggtggtgg tcggtaactt atccgacggt
     1621 gcgtttaaga ccaccgccgt attaagcggt tactcttcag gtactacctg gctggtgttc
     1681 tcggcgttta ccttaagcgc cgcatttgtg accaccggtt taggtaaacg tattgcctat
     1741 ctgctgattg gtaaaatcgg taacaccacg ctgggtctgg gttacgttac ggtattcctc
     1801 gatctggtat tggctccggc aacaccgtct aacaccgcgc gtgcgggcgg cattgtgtta
     1861 ccgatcatca acagcgtggc ggtggctttg gggtccgaac cggaaaaaag tccgcgtcgt
     1921 gtcggacatt acctgatgat gtccatttac atggtcacca aaaccaccag ctatatgttc
     1981 tttaccgcaa tggcggggaa cattctggcg ctgaaaatga tcaacgacat tctgcacctg
     2041 caaattagct ggggtggatg ggcgctggcc gccggattgc cgggcatcat tatgctgctg
     2101 gtcaccccgc tggtgattta caccatgtat ccaccagaaa ttaagaaggt ggataacaaa
     2161 accatcgcta aagcgggcct tgccgaactg ggaccgatga aaatccgcga aaaaatgctg
     2221 ctcggtgtct ttgtgctggc gctgctgggc tggattttca gtaagtctct gggggttgat
     2281 gaatcttcag tggctattgt tgttatggcg accatgctgc tgctgggtat cgttacctgg
     2341 gaagacgtgg ttaaaaataa aggcggctgg aataccttaa tctggtacgg cggtattatc
     2401 ggcttaagct ccttattatc gaaagttaaa tttttcgaat ggttagctga agtctttaaa
     2461 aataacctgg catttgatgg tcacggtaac gttgctttct tcgttattat tttcctcagc
     2521 atcatcgtgc gttatttctt cgcttccggt agtgcctata tcgttgctat gttaccggta
     2581 tttgccatgc tggcgaacgt ctccggcgca ccgttaatgt taaccgcgct ggcactgttg
     2641 ttctccaact cctatggcgg catggttact cactatggcg gcgcggcagg tccggtcatc
     2701 tttggcgtgg gttataacga tattaaatcc tggtggttgg tcggtgcggt actgacgata
     2761 ttaaccttcc tggtgcatat caccctcggc gtgtggtggt ggaatatgct gatcggctgg
     2821 aacatgctgt aaatatactc gtcatacttc aagttgcatg tgctgcgtct gcgttcgctc
     2881 accccagtca cttacttatg taagctcctg gggattcact cgcttgtcgc cttcctgcaa
     2941 ctcgaattat ttagagtata tccatttatt ttcttgctgc gcacttcacg gtgcgcagat
     3001 atctggagca tttgatgatc aagttatctg aaaaaggcgt gtttctcgcc agtaataacg
     3061 aaataattgc cgaagaacat ttcaccggcg aaattaaaaa agaagaagca aaaaaaggca
     3121 ctattgcctg gtctattctc tcttctcata atacgtccgg aaatatggat aaacttaaaa
     3181 ttaagtttga ttcattagcc tctcacgata ttacctttgt tggtattgta cagaccgcta
     3241 aagcgtccgg tatggaacgt ttcccgctgc cgtatgtgct gaccaactgc cataactcac
     3301 tctgcgccgt cggcggcacc attaacggtg atgaccatgt ttttggttta tcggcagctc
     3361 agcgttatgg cggtattttt gtgcctccgc atattgcggt catccatcaa tatatgcgtg
     3421 agatgatggc aggtggcggc aaaatgatcc tcgggtcaga cagtcacacc cgttacggtg
     3481 cattagggac aatggcagtc ggtgagggcg gcggcgagtt ggtaaaacaa ttgcttaatg
     3541 acacctggga tatcgactat ccgggagttg ttgcggtgca tctgaccgga aaaccagcgc
     3601 cgtatgtggg gccgcaggat gtggcgttgg ctatcatcgg tgccgtgttc aaaaacggct
     3661 acgtcaaaaa caaagtgatg gaattcgtag gtcccggtgt tgctgcgctc tctaccgatt
     3721 tccgtaacag cgttgacgtt atgaccactg aaacgacctg tttaagttct gtctggcaaa
     3781 ccgatgaaga agtccataac tggctggcgc tgcacggtcg cggccaggat tactgccagc
     3841 ttaaccctca accgatggcg tactacgatg gctgcatcag cgttgattta agcgccatca
     3901 aaccaatgat tgcgctgccg ttccacccga gcaacgtgta tgaaatcgac acactgaacc
     3961 agaacttgac cgacattctg cgtgagattg aaattgagtc cgaacgcgtg gcgcacggta
     4021 aagccaaact ctcgctgctg gacaaagtgg aaaatggtcg cctgaaagtg cagcagggga
     4081 ttatcgcggg ctgttctggc ggtaactacg aaaacgtcat cgcggcggcg aatgcactgc
     4141 gcggtcaatc ctgtggcaat gacaccttct cgctggcggt ttacccgtca tcacagccgg
     4201 tgtttatgga tctcgccaaa aaaggtgtgg tagcagattt gattggcgca ggcgcaatca
     4261 tcagaaccgc gttctgcggc ccatgctttg gcgcgggcga tacgccaatc aacaacggtt
     4321 tgagtattcg ccacaccacg cgtaacttcc cgaaccgcga aggctctaag ccagctaatg
     4381 ggcagatgtc agcggtggcg ttgatggacg ctcgttctat cgctgcgact gcggcaaacg
     4441 gtggctattt aacctctgcc agcgaactcg attgctggga caacgtgccg gagtacgcct
     4501 tcgatgtaac gccgtataaa aatcgcgtct atcaaggctt tgtgaaaggg gcggcccagc
     4561 aaccgctgat ttacggaccg aacattaaag actggccgga attgggtgcg ctgactgaca
     4621 atatcgtcct caaagtgtgc tcgaagatcc tcgacgaagt gaccaccacc gacgaattga
     4681 ttccttccgg tgaaacctct tcttatcgtt caaatccgat tggtctggcg gagtttaccc
     4741 tgtcacgccg cgatccaggt tatgttggca gcagtaaagc gactgctgag ctggaaaatc
     4801 agcgtctggc ggggaatgtc agcgagctga cagaggtgtt tgcgcgcatt aagcagattg
     4861 ctggtcagga gcatattgat ccgctgcaaa ctgaaattgg cagcatggtc tatgcggtga
     4921 aaccaggcga tggttctgcg cgtgaacagg cggcgagttg tcagcgtgtg attggcggtc
     4981 tggcgaatat tgccgaggag tacgcgacta aacgctatcg ttctaacgtc atcaactggg
     5041 ggatgttacc gctgcagatg gtggaagcgc caacctttga agtgggggat tacatttaca
     5101 tccctggcat taaagcggcg ctggataatc cgggtacgac gtttaaaggt tatgtgatcc
     5161 atgaagatgc gccggtaacg gaaattacgc tctatatgga aagtctgact gctgaagagc
     5221 gcgagattat caaggcgggt agtttgatta acttcaataa aaaccgtcag atgtaaaaag
     5281 cgccatgtga atgtaggtcg cattcggcac ttattgtcgg atgcgatgct ggcgcatctt
     5341 atccgaccta caaatcgcat cgaaccgtag gccggataag gcgtttacgc cgcatccggc
     5401 aaatagttaa tagctcttac ttcttcgcct ctgcaaccac tttgctaccc acgccgcggt
     5461 tattgtattc ccacatgcgg ttgtagttag tgtcattcag attgcgctgt atttcgtcgc
     5521 tatcgtcaac gctgccggtg ttacccgcaa acggacgatt agagatcacc gcatcgcccc
     5581 acggtttagc cgtgttaaaa ccttcgttga tggcgctatc acggatcacc acctgaccgt
     5641 tggtattggc atcaacatcc agcgagcggc ccagttgtgc cacgccatca ccggaagcat
     5701 tgaaacggct gtttacggcg aggaaaccgt agtaaatgtt ggacagcgta gccggtgcaa
     5761 acacatacgc ttcttgctga gtacgtgagt tcaccacgcg gaattcggtg ttatcgaaca
     5821 ccactgcgcc gcgaccagaa acgatatcca catccccttc aatgtagctg ttggtcacca
     5881 gcgtacgcgg ctgacgattc gtttccagac ggttctgcac accgctgttg gtgacaaaga
     5941 aggtgttctg acgaccgaga atgttaacgt tgttaatctg tacctggtca ccgtcagtac
     6001 gcagtgccac cgccggatgg ttacccgcat ctacgctatc acccagcgtg ttttcgatgg
     6061 tcaggttttg cagttgcagg ccattgtttt gtgaccagaa gaccgcagaa cacagaacac
     6121 cgatactgtc gctgcgtttg ctctggcagc tatcgtacat ataccacgct ggtttacctg
     6181 gcatatattt gccgcgcggg ttgacgtcgt gacgccagtc ggcagggctc atgcccccat
     6241 caagggaaag cccaatcttc acatcaatcg gtttttcacc tgtaccgtac agagtaattc
     6301 cacctggagc agcagggaca tacaccgttc cctgatactc accaggcatc acggcaatat
     6361 actggcgctt gttggtgcgc ttgataattg ccgcatctac cgccgcctga atcgtggtat
     6421 gcgttacacc ttgagtaccc gccgggccga caacaaagtc aggttgcgca ggcagggtta
     6481 tcggggacgg attccacgct gcagcacctg gtgtcaggga tgcaaaatag tgttgagcat
     6541 cgaaattctg cgcttctttt gccgacagaa tcgggcgaga agaggtacca ggcgcagttt
     6601 gatcagaagg acgttgatcg ggtggtgttg agctacaggc ggtcagcgtc acgccaaaag
     6661 ccaatgccag cgccagacgg gaaactgaaa atgtgttcac aggttgctcc gggctatgaa
     6721 atagaaaaat gaatccgttg aagtctgctt ttttatacta agttggcatt ataaaaaagc
     6781 attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt
     6841 gatttcaatt ttgtcccact ccctgcctct gtcatcacga tactgtgatg ccatggtgtc
     6901 cgacttatgc ccgagaagat gttgagcaaa cttatcgctt atctgcttct catagagtct
     6961 tgcagacaaa ctgcgcaact cgtgaaaggt aggcggatcc ccttcgaagg aaagacctga
     7021 tgcttttcgt gcgcgcataa aataccttga tactgtgccg gatgaaagcg gttcacgacg
     7081 agtagatgca attatggttt ctccgccaag aatctctttg catttatcaa gtgtttcctt
     7141 cattgatatc ccgagagcat caacatgcaa tgttgtaggg atggcaattt ttacgcctgt
     7201 tttgctttgt tcgacataaa gatatccatc tacgatatca gaccacttca tttcgcataa
     7261 atcaccaact cgctgcccgg taacaacagc cagttccatt gcaagtctga gccaacatgg
     7321 tgatgattct gctgcttgat aaattttcag gtattcgtca gccgtaagtc ttgatctcct
     7381 tacctctgat tttgctgcgc gagtggcagc gaccgggttt gttgttatat ggccttcagc
     7441 tattgcctct cggaatgcat cgctcagtgt tgatctgatt aacttggctg acgccgcctt
     7501 gccctcgtct atgtatccat tgagcattgc cgcaatttct tttgtggtga tgtcttcaag
     7561 tggagcatca ggcagccccc tccttattgc tttaattttg ctcatgtaat ttatgagtgt
     7621 cttctgcttg attcctctgc tggcgaggat tttttcgtag cgatcaagcc atgaatgtaa
     7681 cgtaacagaa ttatcactgt tgattctcgc tgtcagaggc ttgtgtttgt gtcctgaaaa
     7741 taactcaata ttggcctgta ttgcttcagt gattgctatc ctcctgtctc ggcctaaacc
     7801 aaactcttta cccgtccttg ggtccctgta gcagtaatat ccattgtttc ttatataaag
     7861 gttagggggt aaatcccggc gctcatgact tcgccttctt cccatttctg atcctcttca
     7921 aaaggctacc tgttactggt cgatttaagt caacctttac cgctgattcg tggaacagat
     7981 actctcttcc atccttaacc ggaggaggga atatcctgca ttcgcgcacc catcgacgaa
     8041 ctgtttcaag gcttcttggg cgtcgctggc gtgcgttcca ctcctgaagt gtcaagtaca
     8101 tcgcaaagtc tccgcaatta cacgcaagaa aaagccgcca tcaggcggct tggtgttctt
     8161 tcagttcttc aattcgaata ttggttatgt ctgcatgtgc tatctgcgcc catatcatcc
     8221 agtggtcata gcagtcattg atgttctctg cttcgataac tctgttgaat ggttctccat
     8281 tccattcacc tgtgactcgg aagtgcattt atcatctcca taaaacaaaa cccgccgtag
     8341 cgagttcaga taaaagaaat cctcgtcagt gcgaggatgc tgttcattgc tgctatacac
     8401 ttttttgctc tcaacgtaag cggtagctca ttctgttggg ttggtgcagt tgcttttagg
     8461 aaatgctatt taccccttaa acgtcggctg aaagagctaa aatccatgca aaaaatttac
     8521 gcaattttgt gtattattgt gcagtaagta atgagctatt ttctgcgcaa aaaatggatg
     8581 gtaaatttgt ccggggcagg aaaaatttta tgggcgctaa acatgaaaaa agattcgtat
     8641 ccttatttga tttgcatgac agttttaggc ctgatcttta ttttcctttt cttctggtgg
     8701 cgggcagata tttacagggt cacgtttctt aatcagagta tatcccacta ttacattctg
     8761 tttagcatgg gaatagcttt tctgttatct ctgttttggg ttaagaaggg gatagtaaaa
     8821 caaagcggct ggaagagtct gtcagcatac cttaaggttt atgcagggat gtgcatattt
     8881 gctggatttt ttctgattat accccttaca acactaactt attttttgcc tggagagaca
     8941 tcgtcttatg ttgcaccgta tcggtatact tccggtagtt caaaaagttg ttctggagct
     9001 gaggtggatg accccgatct acatgagaat attcgcattt gctatccgta tggcaattat
     9061 gagtacgata atattatcta tgttgaaaag aaaattaata cattaggtgc ggtagtaaca
     9121 tatgcacaga ccgcgcgtga tgatactgaa tgagatagta tatagcgggc aagttttagt
     9181 taatttatcg aggtaatata atttacctcg actcgtttat tctggtatta atatttcgct
     9241 ttacgatcga tttt
//
LOCUS       DBFIFI010000173         9186 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.173, whole genome shotgun sequence.
ACCESSION   DBFIFI010000173 DBFIFI010000000
VERSION     DBFIFI010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9186
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.173"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            48..410
                     /locus_tag="N7B39_003784"
     CDS             48..410
                     /locus_tag="N7B39_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2423687.1"
                     /translation="MNEFEKIFNEMNLDRALLPILFRSNRSTVWKYLSGDSTAPASAM
                     SLIMLLQLIQKRNPDLLAEWLTLSDFTIPPEVYLDQPDYWKGWVYTQHKVNKNVLEYL
                     KKHYPDEDQKSMSKGREE"
     gene            complement(589..1047)
                     /gene="ybbJ"
                     /locus_tag="N7B39_003785"
     CDS             complement(589..1047)
                     /gene="ybbJ"
                     /locus_tag="N7B39_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HGU2423688.1"
                     /translation="MMELIVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAVLTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLIIRAVIA"
     gene            complement(1044..1961)
                     /gene="qmcA"
                     /locus_tag="N7B39_003786"
     CDS             complement(1044..1961)
                     /gene="qmcA"
                     /locus_tag="N7B39_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HGU2423689.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            2107..2784
                     /gene="fetA"
                     /locus_tag="N7B39_003787"
     CDS             2107..2784
                     /gene="fetA"
                     /locus_tag="N7B39_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HGU2423690.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            2771..3550
                     /gene="fetB"
                     /locus_tag="N7B39_003788"
     CDS             2771..3550
                     /gene="fetB"
                     /locus_tag="N7B39_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HGU2423691.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(3613..4467)
                     /gene="cnoX"
                     /locus_tag="N7B39_003789"
     CDS             complement(3613..4467)
                     /gene="cnoX"
                     /locus_tag="N7B39_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HGU2423692.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(4528..5337)
                     /gene="ybbO"
                     /locus_tag="N7B39_003790"
     CDS             complement(4528..5337)
                     /gene="ybbO"
                     /locus_tag="N7B39_003790"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HGU2423693.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRRSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(5327..5950)
                     /gene="tesA"
                     /locus_tag="N7B39_003791"
     CDS             complement(5327..5950)
                     /gene="tesA"
                     /locus_tag="N7B39_003791"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HGU2423694.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFLMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            5921..6607
                     /gene="ybbA"
                     /locus_tag="N7B39_003792"
     CDS             5921..6607
                     /gene="ybbA"
                     /locus_tag="N7B39_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HGU2423695.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            6604..9018
                     /gene="ybbP"
                     /locus_tag="N7B39_003793"
     CDS             6604..9018
                     /gene="ybbP"
                     /locus_tag="N7B39_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HGU2423696.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGSENQLDSYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNYVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVTPLKAF
                     LAEHQIVPESFYPVVRARLTAINGKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVTLGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
BASE COUNT         2094 a         2365 c         2525 g         2202 t
ORIGIN      
        1 gataaatctc aatttttaag aggtttacgc caggcaggaa aatttaaatg aatgaatttg
       61 agaagatttt taatgaaatg aatctggacc gggcattgct accgatcctt tttcgttcga
      121 accggtcgac agtgtggaag tatttaagcg gcgatagtac ggctcctgcc tctgccatgt
      181 cgctgattat gctgttgcag ttgatacaga aacgaaaccc cgatctgtta gccgaatggt
      241 taacgctttc tgatttcacc attccgccag aagtttacct ggaccagcct gattactgga
      301 aagggtgggt gtatacgcaa cacaaggtca ataaaaacgt cctggaatat ctaaaaaaac
      361 actatccgga tgaagaccaa aaaagtatga gtaaaggccg ggaagagtaa attgccaatg
      421 ccagttaaca tttaacatgg cggaggcggc ctacggtttt ggcgtgatat gacgcgcccg
      481 cgtcgcatca ggcgtcggtg cacgactgcc ggatgcggcg taaacgcctt atccggccta
      541 cgattcgggc gcagacttcc gggcatgata agacgcgtca gcgtcgcatc aggcgatgac
      601 cgcacggatg atcagcgtta tcccttctat cgcaatgact tcaacatgcg tacctgcgcc
      661 gagatcctcg ctggcgctga caggccatga actgtcaccg acgcgcatat gaccgcgccc
      721 gttgaccagc ggagattcca gcacaaaacg tcggccaatc agctgctgcc cgcgctggtt
      781 taaatgactg tcgctgtgct tttgttcgcg cacccgccgc gacaaccatt tccaccacag
      841 ccaggcggcg agcagcgtca ggacggcaaa catcacccct tgccactccc aacccagcgg
      901 caccagccag accaccaggc cagtaatcac tgctgccacg ccactccaca acaaataacc
      961 atttccgccc agcatctcgg ctgccagcag caaaccgccg agactgagcc agaaaatatg
     1021 tggatgaacg actattaact ccatcatggc tgagtccgct tgttggcgct gtctttcacc
     1081 agctcggcaa tcccggcaat cgaccccatc aggctgctgg cctctaatgg catcatcact
     1141 actttgctgt tactggagga accgatctgc tgtaacgctt cggtgtattt ctgcgctacg
     1201 aagtagttca ccgcctgaat atcaccggag gcgatggctt cagacaccat tttggtggcg
     1261 cgggcttctg cttcagcgga acgttcacgc gcttcagcct gtaaaaacgc cgactgacgt
     1321 tcgccttccg ctttcaggat ttgcgactgt ttttcacctt cggctttgag gatttccgcc
     1381 tgacggatcc cttccgcttc aagaatgtaa gcgcgtttgg tacgttccgc tttcatctgc
     1441 gcgttcattg aagagataag ctctgccggt gggcgcacgt cgcgaatttc aatacgggtg
     1501 actttaatcc cccacgggtt ggtggcctca tcgacaatac gcagcaggcg tgagttgatg
     1561 ctgtcgcgct gagagagcat ttcgtcaagt tccattgaac ccaacacggt acggatgtta
     1621 gtcatggtca ggttgatgat cgccagctcc agattgctga cttcataagc cgcgcgtggc
     1681 gcgtcaatca cctgaataaa acagactgcg tcgatggtaa cgttggcgtt atctttcgag
     1741 ataacttcct gggaagggat atcgagcact tgctccatca tattgatctt gcgaccaatg
     1801 cgatccataa acggcaccac cagactgagc cccggctgta acgttttggt atagcgacca
     1861 aaacgttcta ccgtccattg atagccctgc ggcacgattt tgacgcccgc gccgacaatg
     1921 accagcgcga caaaaatgag aatcgggata aagataagca tcggaaaaac ctcctgttgt
     1981 accgtccata atcagcaaaa ttgctgcttg attaaacaaa ttatacctga ttactgaaag
     2041 agagttcccc cttattcctg cgaaggataa actgttttta gtaaaaatca gaaaaaggga
     2101 acagcgatgc aggaaaatag tcctttgctt cagctacaaa acgtaggata tctggcgggt
     2161 gatgcgaaga ttcttaataa catcaatttt tcgctgcgtg ctggcgaatt taagttaatt
     2221 accggtcctt ctggttgtgg caaaagtacg ctgctaaaaa tagttgcttc attgatcagc
     2281 ccaaccagcg gaacgttact gtttgaaggt gaggatgtca gcacactaaa gccagaaatc
     2341 taccgtcagc aagtctctta ctgcgcccag acgccgacgc tgtttggcga tacggtatac
     2401 gataatctga tctttccctg gcagatccgt aaccagcagc ctgacccggc catttttctc
     2461 gattttcttg aacgcttcgc cttgccggac agcattttga cgaagaatat cgccgagcta
     2521 tctggtggtg aaaaacaacg catctcattg attcgtaacc tgcaatttat gccgaaggtt
     2581 ttattgctgg atgaaatcac cagtgcgctg gatgaaagta ataaacataa cgttaatgag
     2641 atgatccatc gttatgtgcg cgagcaaaat attgccgtgc tgtgggtgac acacgataaa
     2701 gacgaaatta atcatgcgga taaagtgatt acactgcaac cgcatgccgg agaaatgcag
     2761 gaagcacgct atgaactcgc ataatattac taacgaatca ttagcactgg cattaatgct
     2821 ggtagtggtg gcaatcttaa ttagccataa agaaaaactg gcgctggaga aagatattct
     2881 ctggagcgtc gggcgcgcga taattcagct gattattgtt ggctatgtgc tgaagtatat
     2941 tttcagcgtg gatgatgcca gcctgacatt attgatggtg ttatttatct gctttaatgc
     3001 ggcgtggaac gcgcaaaaac gcagtaaata tattgctaaa gcttttatct catcgtttat
     3061 tgccattacg gtcggggcgg gaattaccct ggcggtgctg attctctcag ggtcgattga
     3121 atttatcccg atgcaggtga tccctatcgc cgggatgatt gccggtaacg ccatggtagc
     3181 ggtggggttg tgttacaaca atttagggca acgggtcatt agcgaacagc aacagattca
     3241 ggagaaactg agtcttggcg cgacgccgaa gcaggcttca gcgatattga ttcgcgacag
     3301 tattcgcgcg gctttaattc cgacggtcga ttcagcaaaa acggttggct tagtgagttt
     3361 accaggaatg atgtccgggc tgatatttgc cgggattgat ccggtgaagg cgattaaata
     3421 tcagattatg gtgaccttta tgctgctctc aaccgccagc ttgtcgacca taattgcctg
     3481 ctatttaacc tatcgtaagt tttataattc gcgccaccag ttggtggtga cgcaattgaa
     3541 gaagaaatga tgcgatgccg gatgcgcagc atcgcatccg gcgttgtggt tcatgtgccg
     3601 atcaacggaa tatcaataca acaatgcata cagctggcgg cgatacttcg acgccagtgc
     3661 atcacccgta cccagcgcag cgaggatctc ctggaacgtt ttacgcgtct gaccgtctgc
     3721 ggcggtgaga tctttacgca gatgcccgaa cagcaactcc agcgcctctt cattgcgccc
     3781 aacctgatgc agttgcagcg ccagttgcgt cgccagtgcg gcatcttctg gattctccgc
     3841 cacctgctgt tgcaactgtt gaatttccgg cgtatcagcc gcctgcttca gcagttcgat
     3901 ttgcgccacc agcccctggt agcgggtgtc ctgatcctgc aacggaatgg ttttcagcac
     3961 cgcttccgca tcttcagaac ggttcagcgc aatcagcgtt tctgccagca gcaggccgat
     4021 ctccccgttc tgattcgaca actgccaggc gtctttcagc aatggcaggg catcggtgta
     4081 attgccttcc tgcatcagtt gcatcgcctg ctgcgctttc agctcttctt cgcgcggcag
     4141 cactttatcc agcagggcgc ggatcgcctc ttccggttgc ggcccctgga agccatctac
     4201 cggttgcccg ttctggaaca gatacacggt cggaatcgcg cgcagaccaa actgggcggc
     4261 aatcatctgc tccgcgtcgc agtccagctt cgccagaata aattgcccgt tgtactgcgc
     4321 cgcaaggctt tccagaattg gggttaactg caaacagtgc tggctacgtt cagaccaaaa
     4381 atagaacagc accggagtgg tcatcgactg ttcaagaacc tgttgcaggt tagattcgtt
     4441 aatgttgaca atattttcta cggacatgga gtcgctctct gttgtcgata ttttctttga
     4501 catgggggct taagcgcgcg cttcaactca cccctgcaat attttgtcca tcacgcgccc
     4561 cggcagcagg cgcttaagca ccattaccgc ccaggtcacc agcgttaccg gatagcgcat
     4621 cttcggcttc tcgctaataa aagcatggcg tactttgtcc accaccgctt ccggtcccaa
     4681 cgtaaagcgg gcggcgatgc cgggattttc gactggttta tcactttgcg tctggttgac
     4741 gttgtcggtg aagcgagtac gaatgggacc gggttcgatc aggctgactt taattccgct
     4801 gcggcgcagc tccatgcgca gcgcgtctga ccacgcctcc agcgcatatt tactggccgc
     4861 gtaagcgcca cgacccggtg tggagattaa tcccatcacc gatgaggtca tcacaatacg
     4921 cccttcaccg tgcggcaaca tcgcgggtaa caggcgcatg gtgagctggt gtgcgccgaa
     4981 aaagttggcg gaaaactgct gttccatctg cgcacggctg atggtggaaa gggggccata
     5041 catgccgaat ccggcattgt taaagatccc atacagacaa ttatcggtca gggcgatcac
     5101 ctcgtcggct gcgcgatcaa cactttctgg tgaatccaga tcgatcaaca cgccggtaaa
     5161 tcccatgctg ttcatgcgct caacatcatc cggtttccgg caacctgcca gcacatgaaa
     5221 accctggcgt tttaattcga gcgcgctttc caggccaatt ccactggaac atccggtaat
     5281 taagaccgat ttttgcataa ctttacctgt caggatctcc gttgctttat gagtcatgat
     5341 ttactaaagg ctgcaactgc ttcgccatcc agtcggcaat aaacggctgg gcgtcgcggt
     5401 tgggatgaat accgtcatcc tgcatccatt gtggcttgag gtagacctct tccattaaaa
     5461 agggcagcag cggaacatca aactctttgg cgagtttggg gtaaatggcg ctaaaggctt
     5521 cattataacg gcgaccatag tttgcaggca gacgtatttg cattaacaat ggttcagcgt
     5581 tggcggcttt gacatcctgc aaaatctggc gcagcgtttg ctcggtttgc tgtggctgaa
     5641 aaccacgcaa accgtcattg ccgcccagtt caaccagcac ccaacgcggc tgatgctgtt
     5701 tcagcagagc cggaaggcgc gccagccctt gttgcgaggt gtcgccgctg atgctggcat
     5761 tgactaccga cgttttactc tgccacttat cattcaacaa ggcaggccag gccgcgctgg
     5821 cagacattcg atacccggcg ctcaggctat cacccagaat caataacgtg tccgctgcgg
     5881 cagcacggaa ggttaacagg accagaaaca ggaagggcaa atgccagcgg aaaacattgt
     5941 tgaagttcat catcttaaga agtccgtcgg tcagggggag catgaactct ccatcctcac
     6001 cggagttgag ctggttgtca aacgtggcga gaccatcgca ctggtgggcg agtcgggatc
     6061 gggtaagtca accttgctgg cgatcctcgc cgggctggat gacggcagca gtggcgaagt
     6121 gagtctggtg ggacaaccgc tacataatat ggacgaagaa gcgcgggcaa agttgcgcgc
     6181 gaagcacgtc ggctttgttt ttcagtcatt tatgttaatt cctaccctta acgcgctgga
     6241 aaacgtcgag cttccggcgc tgctgcgcgg tgagagtagc gcggaaagtc gtaacggagc
     6301 gaaagcgttg ctcgaacagt tagggctggg taaacgtctg gatcatcttc cggcacagct
     6361 ttccggcggt gaacagcaac gagtggcgct ggcacgagcc tttaacggtc gacctgatgt
     6421 gctgtttgcc gacgaaccca ccggtaacct tgaccgccag acgggcgata aaattgccga
     6481 cctgctgttt tccctcaacc gtgagcatgg caccacgttg attatggtga cccacgacct
     6541 gcaactggcg gcacgctgcg accgctgctt acggctggtg aacgggcagt tgcaggagga
     6601 agcatgattg cacgctggtt ctggcgcgaa tggcgctcgc cgtcgctatt aattgtctgg
     6661 ctggcgctaa gcctggcggt ggcctgcgtg ctggcgctgg gcaatatcag cgatcgcatg
     6721 gagaagggct taagccaaca aagccgggag tttatggcgg gcgatcgggc gttgcgcagt
     6781 tcgcgcgaag tgccgcaagc gtggctggag gaagcgcaaa agcgcggcct gaaagtcggc
     6841 aagcagctga ctttcgccac catgacattt gcaggcgaca caccgcagct ggcgaacgtt
     6901 aaagcggtgg atgatatcta cccgatgtat ggcgatctgc aaaccaatcc ccctggcctg
     6961 aaaccgcagg cgggcagcgt attgctggcc ccacgcctga tggcactgct taacctgaaa
     7021 acgggcgaca ccattgacgt gggcgatgcc accttgcgga ttgccggaga agtgattcag
     7081 gaaccggatt ccggttttaa ccccttccag atggctccgc gtctgatgat gaatctggcg
     7141 gatgtcgata aaaccggagc cgtgcagccg gggagtcggg tcacctggcg ctataaattc
     7201 ggcggcagcg agaaccagct cgacagctat gagaaatggt tgttacctca gcttaaaccc
     7261 gaacaacgct ggtacggtct ggaacaggac gaaggcgcgc tggggcgatc gatggaacgc
     7321 tcgcaacagt tcctgctgct ttcggcactt ctgaccttgc tgctggcagt ggcagcagtg
     7381 gcggtagcga tgaatcatta ctgtcgcagt cgctacgatc tggtggcgat cctcaaaacg
     7441 ctgggggcag ggcgagcgca actgcgtaag ctaatcgtcg gtcagtggtt gatggtgctg
     7501 acgctttcag ccgttaccgg tggggcgata ggcctgttgt tcgaaaacgt gttgatggtg
     7561 ctgctcaagc ccgttctgcc tgctgcacta ccgccagcca gcctctggcc atggctatgg
     7621 gcgctgggca ccatgacggt catctcgctg ctggtggggc tacgaccata ccgcttgttg
     7681 ctggcaacgc agcctttacg cgtattacgt aattatgtgg ttgcgaacgt ctggccgctg
     7741 aagttttatc tgccgattgt cagtgtggtg gttgtgctgc tgctcgccgg attaatgggt
     7801 ggcagcatgc tgctttgggc ggtgctggcg ggcgcggtag tactggcttt gctgtgcggt
     7861 gtactgggct ggatgctgct gaatgtactt cgccgcatga cgctgaaatc gctgcctctg
     7921 cgcctggcgg ttagccgcct gttacgtcag ccgtggtcaa cgttaagcca gctttcggca
     7981 ttttcgctct cctttatgct gctggcactg ctactggtgc tgcgtggcga tctgctcgac
     8041 cgctggcagc agcagttacc gccagaaagc ccgaactact ttttaatcaa catcgccaca
     8101 gaacaggtta cgccgctaaa agcgttcctc gcggaacatc agatagtccc ggaatcgttt
     8161 tatccggtgg tgcgagcgcg gctgacggcg attaacggca agccgacaga aggcaatgaa
     8221 gatgaggcgc tcaaccgcga actcaatctt acctggcaaa atacgcggcc cgatcataat
     8281 ccgattgtcg ccggtaactg gccgccaaaa gctgatgaag tttcgatgga agaggggctg
     8341 gcgaaacgct taaacgttac cctcggcgat accgtgactt ttatgggcga tacccaggag
     8401 ttccgcgcta aagtgaccag cctgcgcaaa gtggactggg aaagtctgcg gcctaatttc
     8461 tattttattt tccctgaagg ggcattagac gggcaaccgc agagctggct taccagtttc
     8521 cgctgggaga atggcaacgg catgttgacg caactcaacc gccagttccc taccattagc
     8581 ctgttagaca ttggcgcgat tttaaaacag gtcggtcagg tgctggagca ggtaagtcgg
     8641 gcgctggagg tgatggtggt gctggtcacc gcctgcggta tgttgctgtt gctggcacag
     8701 gtgcaggtgg gaatgcgtca gcgtcatcag gagctggtgg tgtggcgcac actcggtgcg
     8761 gggaaaaaac tgctgcgtac cacgttgtgg tgtgagttcg ccatgcttgg gtttgtttcc
     8821 ggcctggtgg ccgcaattgg tgcggaaacg gcactggcag tgttgcaggc gaaagtgttt
     8881 gattttccgt gggagccaga ctggcgattg tggattgttc tgccgtgcag cggtgcgctg
     8941 ctgctgtcgc ttttcggcgg ctggctgggc gcgagactgg ttaagggtaa ggcgctgttc
     9001 aggcagtttg cggggtgatg agagtaaata aggggcgttc tggttgaatg gaacgccttg
     9061 tttaaattaa ttctacaata tgtttcattg atgatgttat tgaattggtg atttcctatc
     9121 tttctattgc tgatattaat ctgaatcggt ggagtttaat aattttaaat atagccctac
     9181 aaaaat
//
LOCUS       DBFIFI010000174         8942 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.174, whole genome shotgun sequence.
ACCESSION   DBFIFI010000174 DBFIFI010000000
VERSION     DBFIFI010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8942
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.174"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..788
                     /locus_tag="N7B39_003794"
     CDS             <1..788
                     /locus_tag="N7B39_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HGU2423697.1"
                     /translation="RGLMLLNMLNKLDGGASVGLLAELSGLHRTTVRRLLETLQEEGY
                     VRRSPSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLREVVWPTDVSTLDVDAM
                     VVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQERKELIEMLASRPGDDY
                     QLAREPLKLEAILARARKEGYGQNYRGWDQEEKIASIAVPLRSEQRVIGCLNLVYMAS
                     AMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHLR"
     gene            903..1679
                     /locus_tag="N7B39_003795"
     CDS             903..1679
                     /locus_tag="N7B39_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HGU2423698.1"
                     /translation="MKITQIRNATQIIQYAGKTFLIDPLLAPKGSYPGFAGTARAEIR
                     NPTVELPCDLTTLLNVDALIVTHLHEDHWDETASRVVPKDKLIYVQNEQDAESLNKQG
                     FTHLTVLTDETKFGDITLHKTTGQHGSDRTYADPAMAKRLGAACGVVFQHPTEKTLYL
                     AGDTLWRDDVEATMLTFQPEVVVLNAGFAHVLGHGAIIMGAEDILKTHFTLPEAQIIA
                     THMEAVNHCLLTRDALKEYARDNQIIEFVNVPEDGETLTF"
     gene            1699..2652
                     /locus_tag="N7B39_003796"
     CDS             1699..2652
                     /locus_tag="N7B39_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HGU2423699.1"
                     /translation="MRPLTVAIIAVAGFSPFHLSVPFIVFSEKMAEKKRFHVIICAEK
                     PGNVDSADGFSVTATHDYTAVIQADIVIIPYWGTITQKPPQKLLEALTTARDNGAQIV
                     GLCLGTFVLGYAGLLKNKRAATHWEFEREFQARFPQTHLDINALYVDDDGIITSAGTA
                     AALDCCLYIVRQHFGSDYANHIARRMVVPPYRTGGQAQFIEQPVPKNTHDERINLLLD
                     YLRQNIAQQHDLDSLAQRVMMSRRTLTRHFMKATGSSIAEWLITERLRRSQELLGSSQ
                     LPVERIAAEVGFLSPVTWRQHFKSHFGVSPAEWRKTFRGMA"
     gene            2689..3771
                     /gene="lacI"
                     /locus_tag="N7B39_003797"
     CDS             2689..3771
                     /gene="lacI"
                     /locus_tag="N7B39_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HGU2423700.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPKT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            3894..6968
                     /gene="lacZ"
                     /locus_tag="N7B39_003798"
     CDS             3894..6968
                     /gene="lacZ"
                     /locus_tag="N7B39_003798"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HGU2423701.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TNRPSQQLRSLNGEWQFVWFPAPEAVPESWLECDLPDADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLLSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFHVATLFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLGLNVENPKLWSAEIPNIYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTSATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGEMRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDLVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGRTIEVTSEYLFHHSDNELLHWTVALDGKPLASGEVPLDVAPQGKQVIE
                     LPELPRLESTGQLWLTVHVVQPNATAWSEAGHISAWQQWRLAENLSVTLPSAPHAIPQ
                     LTTSETDFCIELDNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTVHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            7020..8273
                     /gene="lacY"
                     /locus_tag="N7B39_003799"
     CDS             7020..8273
                     /gene="lacY"
                     /locus_tag="N7B39_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HGU2423702.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAILLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            8339..>8942
                     /gene="lacA"
                     /locus_tag="N7B39_003800"
     CDS             8339..>8942
                     /gene="lacA"
                     /locus_tag="N7B39_003800"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HGU2423703.1"
                     /translation="MNMSMTERIKAGKLFTDMCEGLPEKRLRRKTLMYEFNHSHPSEV
                     DKRESLIKEMFATVGENAWVEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSIVTKDIPPNVVAAGVPCRVIREINDRDKQYYFRDYKVES"
BASE COUNT         2103 a         2285 c         2375 g         2179 t
ORIGIN      
        1 cccgcggtct aatgttatta aatatgttaa ataaacttga cggcggtgcc agcgtcgggc
       61 tgctggcgga actcagcggc ctgcatcgca ccactgtgcg gcgactgctg gagacgctgc
      121 aggaagaggg atatgtccgg cgtagtccct ccgatgatag ttttcgtctg accatcaaag
      181 tgcggcaatt aagcgaagga tttcgtgacg aacagtggat ttctgcactg gcggccccac
      241 tgctgggcga tctgttgcgc gaagtggtat ggccgacaga tgtgtccacg ctggatgttg
      301 atgcaatggt ggtacgcgaa accactcacc gtttcagccg cttatccttt caccgggcaa
      361 tggtcgggcg gcgtttaccg cttctgaaaa ccgcctcggg cctgacctgg ctggcctttt
      421 gcccggaaca agaacgcaag gaattaatcg agatgttagc ctcccgcccc ggtgatgact
      481 atcaactggc acgagaaccg ttaaagctgg aagccattct ggcgcgcgcg cgcaaagagg
      541 gttacggaca gaactaccgc ggctgggatc aggaggagaa gatcgcctct atcgccgtac
      601 cgctgcgcag tgaacaacgg gtgattggct gtctgaatct ggtgtatatg gcgagcgcaa
      661 tgaccatcga acaggcagcg gaaaagcatc ttccggcact acaacgggta gcaaaacaga
      721 tcgaagaagg ggttgaatcg caggctattc tggtggccgg acggcgaagc ggcgtgcatt
      781 tacgctagtt acgccaatgt gtaatttggc ccattctcat cgttattcgc ccatttgccc
      841 acatgcacag tacgggctgc acgctacgct aatgacatca atactgtgat gtggagcaaa
      901 tcatgaaaat tacgcaaatt cgtaatgcca cccaaattat ccaatacgct ggcaaaacat
      961 ttcttatcga cccgttactc gcgcctaaag gaagttatcc gggttttgcc ggaacggcac
     1021 gcgcagaaat ccgcaatcca acggtagaat taccgtgcga tttaacaacg ttactcaacg
     1081 ttgacgccct aatcgtgacc catttgcatg aagatcactg ggacgaaaca gcctcccgcg
     1141 tcgtccctaa agataaactg atttatgtgc aaaatgagca agatgctgaa tcactgaata
     1201 aacaaggatt tacgcatctt accgtactta ccgacgagac taaatttggc gatattactc
     1261 tgcataaaac gacagggcag cacggctccg atcgcaccta tgccgacccg gcgatggcga
     1321 agcgtttagg cgcagcttgc ggcgttgttt tccagcaccc gacagagaaa acgctctatc
     1381 tggctggcga taccctctgg cgtgacgacg tcgaggctac aatgctaact ttccaaccag
     1441 aagtggttgt gttgaatgcc ggatttgcgc atgttctggg gcatggcgcg ataattatgg
     1501 gagcagaaga tattctcaaa acacatttta ccctcccaga agcgcaaatt atcgcgacac
     1561 atatggaggc cgtaaaccac tgtttgctga cgcgtgatgc gctcaaggaa tacgccagag
     1621 ataatcaaat tatcgaattt gtgaacgtac cagaagatgg tgaaacgttg acattctaat
     1681 tcggagataa agagtgttat gcgcccgcta actgttgcca ttatcgccgt tgccggtttt
     1741 agtccttttc acctttccgt accgtttatc gtgtttagtg aaaagatggc ggagaaaaaa
     1801 cgctttcacg tgattatttg cgctgaaaag ccgggaaacg tggactctgc cgatgggttt
     1861 tccgtaaccg ccacccatga ctataccgca gtcatccagg cagatattgt gataattcct
     1921 tactggggaa ccattacaca aaaaccgcca caaaaactgc tggaagcctt aacgaccgca
     1981 cgggataacg gcgcacagat tgtcgggctt tgcctgggca cgtttgtgct cggctatgca
     2041 ggtttactga aaaataagcg tgccgccacg cactgggagt tcgagcgtga atttcaggca
     2101 cgttttccac aaacacatct ggatattaac gcgttgtacg tagacgatga cggcattatt
     2161 acctctgccg gtactgccgc ggcgctggat tgctgtttgt atattgttcg gcaacatttt
     2221 ggcagcgact atgctaacca tattgcccga cggatggtcg taccgccata tcgcaccggc
     2281 ggtcaggcgc agtttattga gcaaccggtg ccgaaaaata cccatgatga acgcataaac
     2341 ctcctgctgg attacctgcg gcaaaacatt gcgcaacagc atgatctcga ctcgctggcg
     2401 cagcgagtaa tgatgagtcg ccgcacatta actcgccatt ttatgaaagc gaccggttcg
     2461 agtatcgccg aatggctcat tactgaacgc ttacgccgta gccaggaact gttgggatcc
     2521 agtcagttgc ccgttgagcg gatagcggct gaggtgggtt ttctctcacc tgtgacctgg
     2581 cgtcagcatt ttaaatctca cttcggcgtc agccccgccg aatggcgcaa aacctttcgc
     2641 ggtatggcat gatagcgccc ggaagagagt caattcaggg tggtgaatgt gaaaccagta
     2701 acgctatacg atgtcgcaga gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg
     2761 aatcaggcca gccacgtttc tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag
     2821 ctgaattaca ttcccaaccg cgtggcacaa caactggcgg ggaaacagtc gttgctgatt
     2881 ggcgttgcca cctccagtct ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa
     2941 tctcgcgccg atcaactggg tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc
     3001 gaagcctgta aagcggcagt gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt
     3061 aactatccgc tggatgacca ggatgccatt gctgtggaag ctgcctgcgc taatgttccg
     3121 gcgttatttc ttgatgtctc tgaccagact cccatcaaca gtattatttt ctcccatgaa
     3181 gacggtacgc gactgggcgt ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg
     3241 ttagcgggcc cattaagttc tgtctcggcg cgtctgcgtc tggcgggctg gcataaatat
     3301 ctcactcgca atcaaattca gccgatagcg gaacgggagg gcgactggag tgccatgtcc
     3361 ggttttcaac aaaccatgca aatgctaaat gagggcatcg ttcccactgc gatgctggtt
     3421 gccaacgatc agatggcgct gggcgcaatg cgcgccatta ccgagtccgg gttgcgcgtt
     3481 ggtgcggata tctcggtagt gggatacgac gataccgaag acagctcgtg ttatatcccg
     3541 ccgttaacca ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg
     3601 ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg
     3661 aaaagaaaaa ccaccctggc gcccaagacg caaaccgctt ctccccgcgc gttggccgat
     3721 tcattaatgc agctggcacg acaagtttcc cgactggaaa gcgggcagtg agcgcaacgc
     3781 aattaatgtg agtcagctca ctcattaggc accccacgct ttacactcta tgcgtccggc
     3841 tcgtatgttg tgtgaaattg tgagcggata acaatttcac acaggataca gctatgacta
     3901 tgattacaga ttcactggcc gtcgtattac aacgtcgtga ctgggaaaac cctggcgtta
     3961 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     4021 cccgcaccaa tcgcccttcc cagcagttgc gcagcctgaa tggtgagtgg caatttgtct
     4081 ggtttccggc accagaagcg gttccggaaa gctggctgga gtgcgatctt cctgacgccg
     4141 atactgtcgt cgtcccctca aactggcaga tgcacggtta cgacgcgccc atctacacca
     4201 acgtgacata tcccattacg gtcaatccgc catttgttcc cacggagaat ccgacgggtt
     4261 gttactcgct cacatttaat gttgatgaaa gctggctaca ggaaggccag acgcgaatta
     4321 tttttgatgg cgttaactcg gcgtttcatc tgtggtgcaa cgggcgctgg gtcggttacg
     4381 gccaggacag tcgtttgctg tctgaatttg acctgagcgc atttttacgc gccggagaaa
     4441 accgcctcgc ggtgatggtg ctgcgctgga gtgacggcag ttatctggaa gatcaggata
     4501 tgtggcggat gagcggcatt ttccgtgacg tctcgttgct gcacaaaccg accacacaaa
     4561 tcagcgattt ccatgttgcc actctcttta atgatgattt tagccgcgcg gtactggagg
     4621 cagaagttca gatgtacggc gagctgcgcg atgagctgcg ggtgacggtt tctttgtggc
     4681 agggtgaaac gcaggtcgcc agcggcaccg cgcctttcgg cggtgaaatt atcgatgagc
     4741 gtggcggtta tgccgatcgc gtcacactag gtctgaacgt cgaaaacccg aaactgtgga
     4801 gcgccgaaat cccgaatatc tatcgtgcgg tggttgaact gcacaccgcc gacggcacgc
     4861 tgattgaagc agaagcctgc gatgtcggtt tccgcgaggt gcggattgaa aatggtctgc
     4921 tgctgctgaa cggcaagccg ttgctgattc gcggcgttaa ccgtcacgag catcatcctc
     4981 tgcatggtca ggtcatggat gagcagacga tggtgcagga tatcctgcta atgaagcaga
     5041 acaactttaa cgccgtgcgc tgttcgcatt atccgaacca tccgctgtgg tacaccctgt
     5101 gcgaccgcta cggcctgtat gtggtggatg aagccaatat tgaaacccac ggcatggtgc
     5161 caatgaatcg tctgaccgat gatccgcgct ggttaccggc catgagcgaa cgagtaacac
     5221 gaatggtgca gcgcgatcgt aatcacccga gtgtgatcat ctggtcgctg gggaatgagt
     5281 caggccacgg cgctaatcac gacgcactct atcgctggat taaatctgtc gatccatccc
     5341 gcccggtgca gtatgaaggc ggcggagccg acacctccgc aaccgatatt atttgcccga
     5401 tgtacgcgcg cgtggatgaa gaccagccct tcccggctgt gccgaaatgg tccatcaaaa
     5461 aatggctttc gctgcctgga gaaatgcgcc cactgatcct ttgcgaatac gcccacgcga
     5521 tgggtaacag tcttggcggc ttcgctaaat actggcaggc gtttcgtcag tacccccgtt
     5581 tacagggcgg cttcgtctgg gacttggtgg atcagtcgct gattaaatat gatgaaaatg
     5641 gcaatccgtg gtcggcttac ggcggtgatt ttggcgatac gccgaatgat cgccagttct
     5701 gcatgaacgg tctggtcttt gccgaccgca cgccgcatcc ggcgctgacg gaagcaaaac
     5761 accagcagca gtttttccag ttccgtttat ccgggcgaac catcgaagtg accagcgaat
     5821 acctgttcca tcatagcgat aacgagctcc tgcactggac ggtggcgctg gatggtaagc
     5881 cgctggcaag cggtgaagtg cctctggatg tcgctccgca aggtaaacag gtaattgaat
     5941 tgcctgaact accgcgactg gagagcaccg ggcaactctg gctaacggta cacgtagtgc
     6001 aaccgaacgc gaccgcatgg tcagaagccg gacacatcag cgcctggcag cagtggcgtc
     6061 tggcggaaaa cctcagcgtg acactcccct ccgcgcccca cgccatcccg caactgacca
     6121 ccagcgaaac ggatttttgc atcgagctgg ataataagcg ttggcaattt aaccgccagt
     6181 caggctttct ttcacagatg tggattggcg ataaaaaaca actgctgacg ccgctgcgcg
     6241 atcagttcac ccgcgcaccg ctggataacg acattggcgt aagtgaagcg acccgcattg
     6301 accctaacgc ctgggtcgaa cgctggaagg ctgcgggcca ttaccaggca gaagcggcgt
     6361 tgttgcagtg cacggcagat acacttgccg acgcggtgct gattaccact gtccacgcat
     6421 ggcagcatca gggaaaaacc ttatttatta gccggaaaac ctaccggatt gatggtagtg
     6481 gtcaaatggc gattaccgtt gatgttgaag tagcgagcga tacaccgcat ccggcacgga
     6541 ttggcctgac ctgccagctg gcgcaggtag cagagcgggt aaactggctc ggattagggc
     6601 cgcaagaaaa ctatcccgac cgccttactg ccgcctgttt tgaccgctgg gatctgccat
     6661 tgtcagacat gtataccccg tacgtcttcc cgagcgaaaa cggtctgcgc tgcgggacgc
     6721 gcgaattgaa ttatggccca caccagtggc gcggcgactt ccagttcaac atcagccgct
     6781 acagccaaca acaactgatg gaaaccagcc atcgccatct gctgcacgcg gaagaaggaa
     6841 catggctgaa tatcgacggt ttccatatgg ggattggtgg cgacgactcc tggagcccgt
     6901 cagtatcggc ggaattccag ctgagcgccg gtcgctacca ttaccagttg gtctggtgtc
     6961 aaaaataata ataaccgggc aggccatgtc tgcccgtatt tcgtgtaagg aaatccatta
     7021 tgtactattt aaaaaacaca aacttttgga tgttcggttt attctttttc ttttactttt
     7081 ttatcatggg agcctacttc ccgtttttcc cgatttggct acatgacatc aaccatatca
     7141 gcaaaagtga tacgggtatt atttttgctg ctatttctct gttctcgcta ttattccaac
     7201 cgctgtttgg tctgctttct gacaaactcg ggctgcgcaa atacctgctg tggattatta
     7261 ccggcatgtt agtgatgttt gcgccgttct ttatttttat cttcgggcca ctgttacaat
     7321 acaacatttt agtaggatcg attgttggtg gtatttatct tggcttttgt tttaacgccg
     7381 gtgcgcccgc agtagaggca tttatcgaga aagtcagccg tcgcagtaat ttcgaatttg
     7441 gtcgcgcgcg gatgtttggc tgtgttggct gggcgctgtg tgcctcgatt gtcggcatca
     7501 tgttcaccat caataatcag ttcgttttct ggctgggttc tggctgtgca ctcatcctcg
     7561 ccattttact ctttttcgcc aaaacggatg cgccctcttc cgccacggtt gccaatgcgg
     7621 taggtgccaa ccattcggca tttagcctta aactggcgct ggaactgttc agacagccaa
     7681 aactgtggtt tttgtcactg tatgttattg gcgtttcctg cacctacgat gtttttgacc
     7741 aacagtttgc taatttcttt acttctttct ttgccaccgg tgaacagggt acgcgggtat
     7801 ttggctacgt aacgacaatg ggcgaattac ttaacgcctc aattatgttc tttgcgccac
     7861 tgatcattaa tcgcatcggt gggaaaaatg ccctgctgct ggctggcact attatgtctg
     7921 tacgtattat tggctcatcg ttcgccacct cagcgctgga agtggttatt ctgaaaacgc
     7981 tgcatatgtt tgaagtaccg ttcctgctgg tgggctgctt taaatatatt accagccagt
     8041 ttgaagtgcg tttttcagcg acgatttatc tggtctgttt ctgcttcttt aagcaactgg
     8101 cgatgatttt tatgtctgta ctagcgggta atatgtatga aagcatcggt ttccagggcg
     8161 cttatctggt gctgggtctg gtggcgctgg gcttcacctt aatttccgtg ttcacgctta
     8221 gcggccccgg cccgctttct ctactgcgtc gtcaggtgaa tgaagtcgct taagcaatca
     8281 atgtcggatg cggcgcgagc gccttatccg accaacatat cataacggaa tgatcgcaat
     8341 gaacatgtcg atgacagaaa gaataaaagc aggcaagcta tttaccgata tgtgcgaagg
     8401 cttacctgaa aaaagacttc gtagaaaaac gttaatgtat gagtttaatc actcgcatcc
     8461 atcagaagtt gataaaagag aaagcctgat taaagaaatg tttgccacgg taggggaaaa
     8521 cgcctgggta gaaccccccg tctatttctc ttacggttcc aacatccata taggccgcaa
     8581 tttttatgca aatttcaatt taaccattgt cgatgactac acggtaacaa tcggtgataa
     8641 cgtattgatt gcacccaacg ttactctttc cgttacggga caccctgtac accatgaatt
     8701 gagaaaaaac ggtgagatgt actcttttcc gataacgatt ggcaataacg tctggatcgg
     8761 aagtcatgtg gttattaatc caggcgtcac cattggggat aattctgtta ttggcgcggg
     8821 tagtatcgtc acaaaagaca ttccaccaaa cgtcgtggcg gctggcgttc cttgtcgggt
     8881 tattcgcgaa ataaacgacc gggataagca atattatttc agagattaca aagttgaatc
     8941 gt
//
LOCUS       DBFIFI010000175         8848 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.175, whole genome shotgun sequence.
ACCESSION   DBFIFI010000175 DBFIFI010000000
VERSION     DBFIFI010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8848
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.175"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..72
                     /locus_tag="N7B39_003801"
     CDS             <1..72
                     /locus_tag="N7B39_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2423704.1"
                     /translation="SGFRNAEYIGIRNWLAGIRIKGE"
     gene            74..520
                     /locus_tag="N7B39_003802"
                     /pseudo
     CDS             74..520
                     /locus_tag="N7B39_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749537.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            540..755
                     /locus_tag="N7B39_003803"
     CDS             540..755
                     /locus_tag="N7B39_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2423705.1"
                     /translation="MAIAASYTMHLYCDCRQCTNGKYQTPDFGEYIGTSWAGCAKEAR
                     KDGWRINKDKTRAFAPGHKILRSNKGE"
     gene            757..975
                     /locus_tag="N7B39_003804"
     CDS             757..975
                     /locus_tag="N7B39_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HGU2423706.1"
                     /translation="MPTLFRKEYPRKSRATEFLFLILFIVLMTPISPLIFVWAIGKII
                     ELVIELYNDVVWASFNTLHNKINPYKEN"
     gene            977..1264
                     /locus_tag="N7B39_003805"
     CDS             977..1264
                     /locus_tag="N7B39_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2423707.1"
                     /translation="MATLQELIDLTPEQEKAWNRLVKAVKDFRAAGGKFYSVLDTLSA
                     YNGEHVASIDNDKGYHTASVYMPSIDAPGLTSWADDWHGITLKDGVEVDKD"
     gene            1268..1399
                     /locus_tag="N7B39_003806"
                     /pseudo
     CDS             1268..1399
                     /locus_tag="N7B39_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311036.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1412..1891
                     /locus_tag="N7B39_003807"
     CDS             1412..1891
                     /locus_tag="N7B39_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HGU2423708.1"
                     /translation="MGIELVKYGCNTFICPDNSMEPGNVPLYIGLPRIDPASQTAKLS
                     FQEWLSEQKEKIDVDCGCVSIETLTHWMKSAYEAGNSPVTPDSWISCSDRMPEKGQNV
                     LISVNFDSDLVEPLICSARYTGSTFRRGEATIKPGNGVEQVTHWMPLPEPPQEVNRG"
     gene            2247..2513
                     /locus_tag="N7B39_003808"
                     /pseudo
     CDS             2247..2513
                     /locus_tag="N7B39_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203836.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
     gene            2781..3410
                     /locus_tag="N7B39_003809"
     CDS             2781..3410
                     /locus_tag="N7B39_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HGU2423709.1"
                     /translation="MTTQLIPVFEGTISNEPTLLVNARDLHGFLEVGKDFSNWIRARL
                     NEYGFVENLDYILFSPNLAKTPGRRRKDYHLTLDTAKELAMVERNEKGRQIRRYFIEC
                     EKKLRQSLLPAPMNINYPLSWFSEHHPYSMMSYVDRKTLNLDVSVLFDMPSPTMRILN
                     ELHSKGYNVDAAVAEFNAFKHLTEEMRRTLQDISRLSDRNSRKGFSLSL"
     gene            3466..3897
                     /locus_tag="N7B39_003810"
     CDS             3466..3897
                     /locus_tag="N7B39_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HGU2423710.1"
                     /translation="MKQIAFYRRSGRPGAFRGLKERVTWMIQSRGRPVTGSEIAEKFG
                     VSLCEFNKVARGLTKGSKVVKIKASEPFTTDTGIVDRFFSLESNPRRDTPRSRNAVPP
                     FSRRSREHAAKNCREEYVQKAERRRRLIKAGLYIDEFENAL"
     gene            3894..4520
                     /locus_tag="N7B39_003811"
     CDS             3894..4520
                     /locus_tag="N7B39_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HGU2423711.1"
                     /translation="MTKKYTLIYADPPWTFRDKATDGQRGASFKYPVMSLLDICRLPV
                     WELAAENCLLAMWWVPTQPLEALKVVEAWGFRLVTMKGLTWNKCGKRQTDKLVMGMGS
                     TTRANSEDCLFAVKGNLPERINAGIIQSFTAPRLDHSRKPDMAREKLVQLLGDVPRIE
                     LFARHTSHGFDVWGNQCGTPSIEMVPGIVKFLEKTNERKNDVDKGITS"
     gene            4480..4692
                     /locus_tag="N7B39_003812"
     CDS             4480..4692
                     /locus_tag="N7B39_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HGU2423712.1"
                     /translation="MSEKTTLTKASPVELRQCLEIANQLARSGIRFVPIPITADAELH
                     LFGEILSRKLDELEKLVEEADTSPTV"
     gene            4728..5153
                     /locus_tag="N7B39_003813"
     CDS             4728..5153
                     /locus_tag="N7B39_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2423713.1"
                     /translation="MNAVLTELNKLGKASAESISKGLNIDLNDVIDTLWKLKNQGVVT
                     VKNGIWQAVAREVDKKPNIASVQPVQPVQPVQPVQPVQPVQPVQPVQPVQHNIIGDLL
                     RKSRKEARRAGRKQKRWEGACKALQELNKYRDLINELSE"
     gene            5518..5769
                     /locus_tag="N7B39_003814"
     CDS             5518..5769
                     /locus_tag="N7B39_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HGU2423714.1"
                     /translation="MFVLIQRGQSFVDANNYPVEICKVTLTQVIYRRLDGRTRATSIG
                     AFNEEFERIEHNELHMIKAEIEKEKHIASLRKMRRTSIN"
     gene            5830..6012
                     /locus_tag="N7B39_003815"
     CDS             5830..6012
                     /locus_tag="N7B39_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU2423715.1"
                     /translation="MEINLMTEKEVSELLQKRRTALYNLRKKHGFPDPVLTHPARYSR
                     QAVEQWLKAGGINRAV"
     gene            complement(5996..7165)
                     /locus_tag="N7B39_003816"
     CDS             complement(5996..7165)
                     /locus_tag="N7B39_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU2423716.1"
                     /translation="MALSDTKLRGLYGKPYSGPAEITDGDGLSVRITPAGTITFQYRY
                     RWNGKPVRLTVGRYPSTSLKDARVIVGEMRALYMKGVNPKNYFAPSDGELTLKECLDQ
                     WWDKYVSGLKPNTQTLYRSVVYNTMYTQFEDSPVAGIPVSAWARFFDKQESLNKKKAR
                     VLLLQLRSVINWCISRQLIPSCELLKLSVKNIGKKPDVGSRVLTYTELAKIWLALENS
                     KVVTSNKLLHQLLLLWGARLSELRLATASEFNMEDLVWTTPKEHSKMGNIIRRPIFTQ
                     VEPYIERLLNAGFDVLFPGQEIDKPIDRSSANLYMKKLREKIDIPEWRTHDFRRSLVT
                     NLSGEGIMPHVTEKMLGHELGGVMAVYNKHDWLSEQKDAYELYADKIFWHAKQLG"
     gene            7361..7435
                     /locus_tag="N7B39_003817"
     tRNA            7361..7435
                     /locus_tag="N7B39_003817"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7394..7396,aa:Arg,seq:cct)
     gene            7597..8754
                     /gene="intS"
                     /locus_tag="N7B39_003818"
     CDS             7597..8754
                     /gene="intS"
                     /locus_tag="N7B39_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HGU2423717.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKRQVWSAAYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLAEAMKGYRKKNFPFLPADQIPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMQWKNVDFENRIITIEASVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKSICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDGKVE"
BASE COUNT         2604 a         2059 c         2077 g         2108 t
ORIGIN      
        1 agcggtttca ggaacgctga atatatcggc ataagaaact ggcttgctgg cattcgcatc
       61 aaaggagagt gatatgacca ctataaccga taagaaacag tatcccagcg agcaatttct
      121 taatgagctg atcaccaaca tggagtttgc tgcaagggca ccagttgaag tcgtgagagc
      181 gatggcagca gagctacaga agcggcgcga agctgatagt gcagaaccag ttgcttatat
      241 tttcaaacat ccggccggga aattattctg ggctttaacg gatgaaagca ataaagagca
      301 agcggacgtt attcctgttt atgctgccgc gcctgcgtcg gttgtgccgg ataatgcatc
      361 agagcctctt gcttatgctt acaaagagct tacgcctgag attatgcgca accatttagc
      421 tgtattcgag cgatatggaa tagccccaaa cgatagcact accacaattc aggcactgcg
      481 aatcgcgctg gatggtatag agcggagcga cgccatgctt cagggtaaag gagggggata
      541 tggctattgc cgcaagttac accatgcatc tctactgtga ttgtcgccag tgtacaaatg
      601 gtaaatatca aacgccagat ttcggtgagt atatcggtac gtcatgggct ggctgtgcaa
      661 aagaggcgcg caaggatggc tggcgaataa acaaagacaa aacgcgtgct tttgcgccag
      721 ggcataaaat tttgaggagc aacaaaggag agtgatgtgc ctacattatt cagaaaagaa
      781 tatccgcgaa aaagtagagc gacagaattt ttgtttctca ttctgtttat cgtgttgatg
      841 acaccgatat ccccgctaat ttttgtctgg gcaatcggga aaataattga gctagttatt
      901 gagttgtata acgacgtggt atgggcgtcg ttcaacacac tgcacaataa aattaaccca
      961 tataaggaaa actgaaatgg caactttgca ggaattaatc gacctgacgc cagaacagga
     1021 aaaagcgtgg aatcgtcttg tgaaggctgt aaaggatttc agggcagccg gaggaaagtt
     1081 ttatagtgtc ctagacacgc tgagcgcata caacggcgag cacgttgcca gcattgataa
     1141 cgataagggc taccacactg caagcgtcta tatgcctagc attgatgcgc ctgggttaac
     1201 cagttgggct gatgattggc acggcatcac gctgaaagat ggcgttgaag tggataagga
     1261 ctaacacatg acaacgttca ccgacaaaga aatgattaaa gaaatcaaag agcgaatcgg
     1321 cagcctggac gtgcgagata atattgagcg ccgtgcttat gaaattgcgt tgactgcatt
     1381 gaccactgaa ccattcgcca ctatcgacac agtgggaatt gagctcgtta aatatggttg
     1441 taacacgttt atttgtcccg acaattcgat ggagccggga aatgtaccac tatatatcgg
     1501 cctgccacga attgatccag caagccagac tgccaagctg tcattccagg aatggttgtc
     1561 agaacaaaaa gaaaaaatag acgttgattg cggatgtgta agcatcgaaa cgcttacgca
     1621 ctggatgaaa tccgcttatg aggctggtaa ctctccggta actccggata gttggataag
     1681 ctgtagtgat cgaatgcctg aaaagggcca gaacgtgctt atttcggtga atttcgatag
     1741 tgatctggtt gagccgctaa tatgctccgc acgctatacc ggaagcacct ttcggcgagg
     1801 agaagcaacg attaagccgg gcaatggtgt tgagcaggta acacactgga tgccgctacc
     1861 agaaccgccg caggaggtta accgtggcta acctgcaact tgccgtcaaa ggtgaatact
     1921 tcgcagatag tttcccccaa atctggggaa aagcccgaat ggcgcggctt acagcaagat
     1981 aaggcctaca tgatttgaca aatccgccag agctatcgca tactgaccgc actagaactt
     2041 caaagcggtc atccgcaccc gatagctttg cggctttttt atgcctgcaa tttggcatag
     2101 tcacatccgt acaaaggtcg ggtggagagg cgtaatacaa tacccgcaag gggaatatgc
     2161 ccggagcatc tttgaaggct ctagttgaca cccgatcacc agccactaac tggtgattgc
     2221 taactaaaac ttcaaaggag gtcatcatga ccagtcaact catccccgta ttcaacggca
     2281 ctatcgacaa cgaaacaatt cttctagtta acgctcgtga gctacactct tttcttggtg
     2341 tgggcagaat gttcgcgcac tgggttaaag agcgcattgc tgaatatgga ttcgttgaaa
     2401 atcaagattt tgttattgct tgccaaaatt ggcaagcaaa aggtagaggc ggtcacaacc
     2461 gcaaggaata ccacctcacc ctcgacacag ccaaagagct tgcgatggtt gacgactgcg
     2521 tccaatactg actatactcc gcgccgaggc ctcgaaaacc tcccaaaagc ggattaaacc
     2581 aaccccgtca gtgttggatt ttttatgcct gttattcagt gatagcacag tgtgcggtca
     2641 catccccgat caatgtcggg agggcggcga atacaacacc cgcaagggga ataagtccgc
     2701 ggtatctttt gggccgtttc gagcctcccg gcaccacatt ttgtggtggc atttctcgaa
     2761 aaaagccaaa ggaggtcatc atgaccactc aactcatccc tgtgtttgaa ggtacaatct
     2821 caaatgaacc tacgcttctg gtgaacgcgc gtgatttgca tggattttta gaggtaggta
     2881 aagatttttc taactggata cgggccaggc taaacgagta tggattcgta gaaaatttag
     2941 actacattct attttcgcca aatttggcga aaactccagg ccgccgccgc aaggattacc
     3001 acctcaccct cgacacagcc aaagaactgg cgatggttga gcgtaacgaa aaaggccgcc
     3061 agatccgccg atacttcatc gagtgcgaaa agaaacttcg ccagagcctt ttacctgcac
     3121 caatgaacat caactaccct ctatcgtggt tttcagagca ccacccctac tccatgatga
     3181 gctatgttga tcgaaaaact cttaacctgg acgtttctgt gctcttcgac atgccaagcc
     3241 caactatgcg catcctcaat gagctacaca gcaaaggcta taacgttgac gccgctgtcg
     3301 ccgaatttaa cgccttcaag catctgacgg aagaaatgcg ccgcacgcta caggatattt
     3361 caagattgtc agatcgaaat tcccgaaaag gcttctcgtt aagcctgtaa cttcccctac
     3421 catccccgac atcccgtcgg ggttttcata tctggagaca aaaatttgaa acagatcgct
     3481 ttctacaggc gttctggaag gcccggtgcc ttccgtgggt taaaagaacg cgtcacctgg
     3541 atgattcagt ctcgtggtcg tcctgttact ggcagtgaaa tagcagagaa atttggtgtt
     3601 tcactttgcg agttcaacaa ggtagctcgc ggcctgacaa agggcagtaa ggttgtgaaa
     3661 ataaaggctt cagaaccatt caccacagac accggaatcg ttgatcgctt tttctccctc
     3721 gaatcaaatc ctcgtcgtga cacacctcgc tcacgcaatg ccgttcctcc attcagtcgc
     3781 agaagccgtg aacacgcagc aaaaaactgt cgcgaggaat acgtgcaaaa ggccgaacgc
     3841 cgtcgccgac tgattaaagc aggactttac attgatgagt ttgaaaacgc gctatgacga
     3901 aaaaatacac cctcatttac gcagatcccc cctggacatt ccgcgacaaa gcaaccgatg
     3961 gtcaacgcgg tgcaagtttt aaatatccgg tcatgagtct tctggatatc tgccgcctcc
     4021 cggtatggga actggcagcc gagaattgcc tgttggctat gtggtgggtg cctacacaac
     4081 cacttgaagc attgaaggtt gtagaagcgt ggggctttcg tctggtgacg atgaaaggat
     4141 taacctggaa caaatgcggg aaaagacaga ccgacaagct ggtcatgggt atgggtagca
     4201 ccactcgcgc taacagcgaa gactgccttt ttgcagtgaa aggaaatctg cccgaacgca
     4261 ttaacgccgg aataatccag tcattcactg caccacgcct tgatcactcc cgcaagccgg
     4321 atatggctcg agaaaaactt gtgcaacttc ttggcgatgt tccccggata gaactgttcg
     4381 cccgccacac ctcgcatgga tttgatgtat ggggtaacca atgcggcaca ccatccattg
     4441 agatggttcc gggtattgtt aaatttctgg agaaaaccaa tgagcgaaaa aacgacgttg
     4501 acaaaggcat caccagttga attaaggcag tgtctggaaa tcgcaaatca acttgccaga
     4561 agtggaatac gatttgttcc aatcccgatt acagcagatg cagaacttca tctgtttggt
     4621 gaaattcttt cccgaaagct ggatgaactg gaaaagctgg tagaagaagc tgacacctca
     4681 ccaaccgtat aacagcccca ccgacattaa aatatcagga gaaaaaaatg aacgcagtgc
     4741 tcacagaatt gaacaaatta ggaaaagcat cagccgaaag tatttctaaa ggtctcaata
     4801 ttgatttgaa tgacgttatt gacactctat ggaagttaaa aaaccagggg gtagtaactg
     4861 taaaaaatgg catctggcag gcagttgcaa gggaagtgga caaaaaacca aatatcgcct
     4921 cagtgcagcc agtgcagcca gtgcagccag tgcagccagt gcagccagtg cagccagtgc
     4981 agccagtgca gccagtgcag ccagtgcagc acaacattat aggcgacctg ctacgtaaat
     5041 cacggaaaga agcgcgccgc gccgggcgga aacagaaacg atgggagggt gcatgtaagg
     5101 cgttgcaaga actgaataaa taccgtgact tgatcaacga attgtcagag tgagcgaatc
     5161 acagagagaa tattttgaga aacaccccgc gtacattaat attgtttttc ctttctctaa
     5221 tgacgggcgg tatcctgtta ttcgcaggaa caacattatt tatttttgtt gcccggctaa
     5281 ctgcgaggat aatgacatga aaatcacatt tgaaagttac ggtttaacag caagtgtggt
     5341 tatatccagc tctatattcg aaagccgcaa acatcagcat atcgttgatg cagtgaaact
     5401 caaagccccg gaagttaccg tcacaacaca tggacttttc aggatacgaa cgacgttgac
     5461 tacaacacac ctctcggcat gccgtgttta tgatatggca ctcaaggaat acaaccagtg
     5521 ttcgtcctga tccagcgcgg gcagtctttc gttgatgcca acaactatcc ggtagaaata
     5581 tgcaaggtaa ctctgactca ggtgatctac cgaaggctcg acggcagaac cagagccact
     5641 tcaattggtg catttaatga agaatttgag cgaatagagc acaacgaact gcatatgatt
     5701 aaagcggaaa ttgagaagga aaagcatatt gccagccttc gaaaaatgcg ccgtacatca
     5761 atcaactaac aaccgccttc gggcggttta ttatgctgtt gaaaaacatg tagaaaagga
     5821 gagcatacta tggaaatcaa cttaatgacc gaaaaagaag tttctgagtt gttacaaaaa
     5881 aggcgcacag ctttgtataa tttgcgaaaa aaacacggat tccctgatcc agtacttact
     5941 catccggcgc gatatagtcg tcaggccgtt gaacaatggc ttaaagccgg aggaattaac
     6001 cgagctgttt agcgtgccag aaaattttat cagcatacaa ctcatacgca tctttctgtt
     6061 ccgacaacca gtcgtgttta ttatacacgg ccataactcc cccaagttca tgccccagca
     6121 ttttttcagt gacgtggggc ataattcctt ccccagataa attcgttacc agagaacgcc
     6181 taaaatcatg cgttctccat tcaggaatat caattttctc ccttaacttt ttcatgtaca
     6241 aattagccga cgaacgatca ataggtttat ctatttcctg cccggggaat agcacatcaa
     6301 aacccgcatt aagcaatcgc tcaatataag gctcaacctg agtaaatatc ggacgccgga
     6361 taatattacc catctttgaa tgctctttag gggttgtcca gaccagatct tccatgttga
     6421 actcactggc tgtagcgaga cgtagctcag aaagcctggc cccccatagc aatagcaatt
     6481 gatgtaacag cttgttggaa gtaaccactt ttgagttctc cagtgccagc cagatctttg
     6541 ccagttccgt ataggtaagt acgcgactcc ccacatctgg tttcttccca atattcttta
     6601 cactaagttt gagtaattcg catgatggga ttaactggcg gctgatacac cagttaataa
     6661 ctgaacgcag ttgtaacaac agaacacgag cctttttctt gttgaggctt tcttgcttgt
     6721 caaaaaagcg agcccaagcc gatacaggga taccggcaac tggcgagtct tcaaactgtg
     6781 tgtacatagt gttgtacaca acagatctat aaagcgtctg agtattgggt ttcaggccgc
     6841 taacatactt atcccaccac tgatccaggc attcttttaa tgtcaattcg ccatcactgg
     6901 gggcaaaata atttttaggg ttaactccct tcatgtacaa tgcgcgcatc tcaccgacga
     6961 taacacgtgc gtccttcagt gaagtggacg gatagcgccc gacagtaaga cgtactggct
     7021 taccattcca gcgataacgg tactgaaacg tgatagttcc tgctggagta atccgcacac
     7081 ttaacccgtc accatcggtg atttcagcag ggccagagta gggttttccg tataggcctc
     7141 gtagtttggt gtcactcagt gccaatgctt tatatcctgt acacaagttt ataatgtatt
     7201 ctgtactcaa tatgtacgca ttagcaagtg aacaaaacac ttttcaacgc aaaagaagat
     7261 aatcaaccat gaacaaaaac gaacttacac cttgatttta aaaccataaa gacggtatta
     7321 tcagtcatat ggtgaacaac caaaatcaat acgcaacaac gtcctcttag ttaaatggat
     7381 ataacgagcc cctcctaagg gctaattgca ggttcgattc ctgcagggga caccatttat
     7441 cagttcgcct ccatccgtac cagtccgcaa aatcccctga atatcaagcc ttccgtagat
     7501 tcacagttcg tcatggttcg cgtcagatcg ttgacagccg cacgccatga cgggtaaaaa
     7561 gtggataaaa taattttacc caccggattt ttacccatgc tcaccgttaa gcagattgaa
     7621 gcagcaaagc cgaaagaaaa accataccgc ctactcgatg gtaatggcct gtacctttat
     7681 gtccctgtat cagggaaaaa ggtatggcag cttcgctaca agattgacgg taaggagaaa
     7741 atcctgaccg tcggaaaata tccgcttatg actttgcaag aggcaaggga taaagcatgg
     7801 actgcgagga aagacatctc ggttggcatc gatccggtaa aggcgaaaaa ggcttcgtct
     7861 aacaacaatt cctttagtgc gatttacaag gaatggtacg agcataagag gcaagtctgg
     7921 tcagccgcct atgcgactga acttgcaaaa atgtttgatg acgacatttt acctatcatc
     7981 ggcggccttg aaattcagga tattgagccg atgcaactgc tggaagtaat ccgcagattt
     8041 gaagatcgcg gggcaatgga gcgagccaac aaagcacgca gaagatgcgg cgaggttttc
     8101 cgttacgcta ttgtcaccgg aagggctaaa tataacccgg cacctgacct tgctgaagcc
     8161 atgaagggat accgcaagaa gaacttcccg tttttacctg ccgaccagat cccggcattc
     8221 aacaaagcac ttgcaacatt ttcaggaagt atcgtatctc tcattgcgac caaagtttta
     8281 cgctacacag cactaagaac gaaagagctt cgttccatgc aatggaagaa cgtcgatttt
     8341 gaaaacagga ttatcaccat cgaggccagt gtgatgaagg gacgcaagat tcatgtggtt
     8401 ccgatgtcgg accaggttgt tgaacttctc actacgctaa gctccatcac taaaccagta
     8461 tcagagtttg tttttgccgg gcgcaacgat aagaagaagt caatctgtga gaacgctgta
     8521 ctgcttgtga tcaaacaaat cggctatgaa ggtctggaaa gcggtcacgg attcaggcat
     8581 gaattcagca cgattatgaa cgagcacgaa tggcctgctg acgctattga agtgcaacta
     8641 gcacatgcca acggcggatc tgtgcgtggg atttacaacc atgctcagta tctcgataag
     8701 cgcagagaaa tgatgcagtg gtgggcggac tggcttgatg ggaaggtgga gtagttatta
     8761 caacgattct tataaaaaag aaaagccttg cacatatagc aaagcttttc acttctcagg
     8821 atttcttaca ccaagaaacc atcaacaa
//
LOCUS       DBFIFI010000176         8841 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.176, whole genome shotgun sequence.
ACCESSION   DBFIFI010000176 DBFIFI010000000
VERSION     DBFIFI010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8841
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.176"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(359..622)
                     /gene="rpsT"
                     /locus_tag="N7B39_003819"
     CDS             complement(359..622)
                     /gene="rpsT"
                     /locus_tag="N7B39_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HGU2423718.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            725..949
                     /locus_tag="N7B39_003820"
     CDS             725..949
                     /locus_tag="N7B39_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HGU2423719.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGSQFLPVICAMTMT
                     MKSHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            957..1898
                     /gene="ribF"
                     /locus_tag="N7B39_003821"
     CDS             957..1898
                     /gene="ribF"
                     /locus_tag="N7B39_003821"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HGU2423720.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1941..4757
                     /gene="ileS"
                     /locus_tag="N7B39_003822"
     CDS             1941..4757
                     /gene="ileS"
                     /locus_tag="N7B39_003822"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HGU2423721.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPDEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFAHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYTEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4757..5251
                     /gene="lspA"
                     /locus_tag="N7B39_003823"
     CDS             4757..5251
                     /gene="lspA"
                     /locus_tag="N7B39_003823"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HGU2423722.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVTLVVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            5339..5788
                     /gene="fkpB"
                     /locus_tag="N7B39_003824"
     CDS             5339..5788
                     /gene="fkpB"
                     /locus_tag="N7B39_003824"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HGU2423723.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5790..6740
                     /gene="ispH"
                     /locus_tag="N7B39_003825"
                     /gene_synonym="lytB"
     CDS             5790..6740
                     /gene="ispH"
                     /locus_tag="N7B39_003825"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HGU2423724.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6806..7720
                     /gene="rihC"
                     /locus_tag="N7B39_003826"
     CDS             6806..7720
                     /gene="rihC"
                     /locus_tag="N7B39_003826"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HGU2423725.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLSTLPQLNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            7887..8708
                     /gene="dapB"
                     /locus_tag="N7B39_003827"
     CDS             7887..8708
                     /gene="dapB"
                     /locus_tag="N7B39_003827"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HGU2423726.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKENGLFDMRDVLDLNNL"
BASE COUNT         2035 a         2185 c         2480 g         2141 t
ORIGIN      
        1 cctgtgttga ttgctgttaa taatttacaa ggtctaatga ggagatattt atgaatggtt
       61 aaagtatata ggaatttaat tcaaaaatag agtggtctta agtttaatac ctcaatgtgt
      121 atcacaatat ccacattctt tgtaggggag tctggagatt gagtagatat tcttgttcag
      181 aatgtatcag ccgatggttc tacgattctt aagccacgaa gagttcagat agtgcaacgg
      241 catgtctctt ttgactatct ggcaaccggc agtgtgttct ctcacgcatc acaaaagcag
      301 caggcataaa aaaacccgct tgcgcgggct ttttcacaaa gcttcagcaa attggcaatt
      361 aagccagttt gttgatctgt gcagtcaggt tagccttatg acgtgcagct ttgtttttgt
      421 ggatcagacc tttagcagcc tgacggtcca cgatcggttg catttcgtta aatgctttct
      481 gtgcagcagc tttgtcgcca gcttcgatag ctgcgtatac tttcttgatg aaagtacgca
      541 tcatagagcg acggcttgcg ttgtgcttac gagccttttc agactgaatg gcgcgcttct
      601 tagctgattt gatattagcc aaggtccaac tcccaaatgt gttctatatg gacaattcaa
      661 aggccgagga atatgccctt ttagccttct tttgtcaatg gatttgtgca aataagcgcc
      721 gttaatgtgc cggcactcgt tacgtagtga tggcgcagga ttctaccagc ttgcggggtg
      781 tgaatacagc ttttccgcga taaaaattgc agcaggcagt cagtttcttc ccgtgatttg
      841 cgccatgacc atgacaatga aaagccactt ctttctgatt tcggtactca atcgccggtt
      901 aaccttgacc gctgtacaag gtatactcgg acgattttca ctgttttgag ccagacatga
      961 agctgatacg cggcatacat aatctcagcc aggccccgca agaagggtgt gtgctgacta
     1021 ttggtaattt cgacggcgtg catcgcggtc atcgcgcgct gttacagggc ttgcaggaag
     1081 aagggcgcaa gcgcaactta ccggtgatgg tgatgctttt tgaacctcaa ccactggaac
     1141 tgtttgctac cgataaagcc ccggcacggc tgacccgact gcgggaaaaa ctacgttacc
     1201 ttgcagagtg tggcgttgat tacgtgctgt gcgtacgttt cgacaggcgt ttcgcggcgt
     1261 taaccgcgca aaatttcatc agcgatcttc tggtgaagca tttgcgggta aaattcctcg
     1321 ccgtggggga tgatttccgc tttggcgctg gtcgggaagg cgatttcttg ttattacaga
     1381 aagctggcat ggaatacggc ttcgatatca ccagtacgca aactttttgc gaaggtggcg
     1441 tgcgtatcag cagcaccgcc gtgcgtcagg cccttgcgga tgacaatttg gctctggcag
     1501 aaagtttact ggggcacccg tttgccatct ccgggcgtgt agtccacggt gatgaattag
     1561 ggcgcactat aggtttcccg acggcgaatg taccgctacg ccgtcaggtt tccccggtga
     1621 aaggggttta tgcggtagaa gtgctgggcc tcggtgaaaa gccgttaccc ggcgtggcaa
     1681 acatcggaac acgcccaacg gttgccggta ttcgccagca gctggaagtg catttgttag
     1741 atgttgcaat ggacctttac ggtcgccata tacaagtagt gctgcgtaaa aaaatacgca
     1801 atgagcagcg atttgcttcg ctggacgaac tgaaagcgca gattgcgcgt gatgaattaa
     1861 ccgcccgcga attttttggg ctaacaaaac cggcttaagc ctgttatgta atcaaaccga
     1921 aacacggaac cgagaatctg atgagtgact ataaatcaac cctgaatttg ccggaaacag
     1981 ggttcccgat gcgtggcgat ctcgccaagc gcgaaccggg aatgctggcg cgttggactg
     2041 atgatgatct gtacggcatc attcgtgcgg ctaaaaaagg caaaaaaacc ttcattctgc
     2101 atgatggccc tccttatgcg aatggcagca ttcatattgg tcactcggtt aacaagattc
     2161 tgaaagacat tatcgtgaag tccaaagggc tttccggtta tgactcgccg tatgtgcctg
     2221 gctgggactg ccacggtctg ccgatcgagc tgaaagtaga gcaagaatac ggtaagccgg
     2281 atgagaaatt caccgccgcc gagttccgcg ccaagtgccg cgaatacgcg gcgacccagg
     2341 ttgacggtca acgcaaagac tttatccgtc tgggcgtgct gggcgactgg tcgcacccgt
     2401 acctgaccat ggacttcaaa actgaagcca atatcatccg cgcgctgggc aaaatcatcg
     2461 gcaatggtca cctgcacaaa ggcgcgaagc cggtgcactg gtgcgtagac tgccgttctg
     2521 cactggcaga agcggaagtt gagtattacg acaaaacttc tccgtccatt gacgtcgctt
     2581 tccaggcggt cgatcaggat gcgctgaaag cgaaatttgg cgtaagcaac gttaacggcc
     2641 caatctcgct ggtgatctgg accactacgc cgtggactct gcctgcgaac cgcgcaatct
     2701 ctattgcacc tgatttcgac tatgcgctgg tgcagatcga cggtcaggcc gtgattctgg
     2761 cgaaagatct ggttgaaagc gtaatgcagc gtatcggcgt gaccgattac accattctcg
     2821 gcacggtaaa aggtgcggag cttgagctgc tgcgctttgc ccatccgttt atgggcttcg
     2881 acgtcccggc aatcctcggc gatcacgtta ccctggatgc cggtaccggt gccgttcaca
     2941 ccgcgcctgg ccacggcccg gacgactatg tgatcggtca gaaatacggc ctggaaaccg
     3001 ctaacccggt tggcccggac ggcacttatc tgccgggcac ttatccgacg ctggatggcg
     3061 tgaacgtctt caaagcgaac gacatcgtcg ttgcgctgct gcaggaaaaa ggcgcgctgc
     3121 tgcacgttga gaaaatgcag cacagctatc cgtgctgctg gcgtcacaaa acgccgatca
     3181 tcttccgcgc aacgccgcag tggttcgtca gtatggatca gaaaggtctg cgcgcgcagt
     3241 ctctgaaaga gatcaaaggt gtgcagtgga tcccggactg gggccaggcg cgtatcgagt
     3301 cgatggtcgc taaccgtcct gactggtgta tctcccgtca gcgtacctgg ggcgtaccga
     3361 tgtctctgtt cgtgcacaaa gacacggaag agctgcatcc gcgtaccctc gaactgatgg
     3421 aagaagtggc taaacgcgtt gaagttgatg gcatccaggc gtggtgggat cttgatgcga
     3481 aagagatcct cggcgatgaa gctgatcagt atgtgaaagt gccggatacg ctggatgtat
     3541 ggtttgactc cggctctact cactcttctg ttgttgacgt gcgcccggaa tttgccggtc
     3601 acgctgcgga catgtatctg gaaggttctg accaacaccg tggctggttc atgtcttctc
     3661 tgatgatctc caccgcgatg aagggcaaag cgccgtatcg tcaggtgctg acccacggct
     3721 ttaccgtaga tggtcagggc cgcaagatgt ctaaatccat cggcaacacc gtgtcgccgc
     3781 aggatgtgat gaacaaactg ggcgcggata ttctgcgtct gtgggtggca tcaaccgact
     3841 acaccggtga aatggccgtt tctgacgaga tcctgaaacg tgctgccgac agctatcgtc
     3901 gtatccgtaa caccgcgcgc ttcctgctgg caaacctgaa cggttttgat ccggcaaaag
     3961 atatggtgaa accggaagag atggtagtac tggatcgctg ggccgtaggt tgtgcgaaag
     4021 cggcacagga agacatcctc aaggcatacg aagcatacga tttccacgaa gtggtacagc
     4081 gtctgatgcg cttctgctcc gttgagatgg gttccttcta cctcgacatc atcaaagacc
     4141 gtcagtacac cgccaaagcg gacagcgtgg cgcgtcgtag ctgccagact gcgctgtatc
     4201 acatcgcaga agcgctggtt cgctggatgg caccaatcct ctccttcacc gctgatgaag
     4261 tgtggggcta cctgccgggc gaacgtgaaa aatacgtctt caccggcgag tggtacgaag
     4321 gtctgtttgg tctggcagac agtgaagcaa tgaacgatgc gttctgggac gagctgttga
     4381 aagtgcgtgg cgaagtgaac aaagtcattg agcaggcgcg tgccgacaag aaagtgggtg
     4441 gctcgctgga agcggcagta accttgtata cagaaccgga actggcggcg aaactgaccg
     4501 cattgggcga tgaattacga tttgtcctgt tgacctccgg cgctaccgtt gcagactata
     4561 acgacgcacc tgctgatgct cagcagagcg aagtgctcaa agggctgaaa gtcgcgttga
     4621 gtaaagccga aggtgagaag tgcccacgct gctggcacta cacccaggat gtcggcaagg
     4681 tggcggaaca cgcagaaatc tgcggccgct gtgtcagcaa cgtcgccggt gacggtgaaa
     4741 aacgtaagtt tgcctgatga gtcaatcgat ctgttcaaca gggctacgct ggctgtggct
     4801 ggtggtagtc gtgctgatta tcgatctggg cagcaaatac ctgatcctcc agaactttgc
     4861 tctgggggat acggtcccgc tgttcccgtc gcttaatctg cattatgcgc gtaactatgg
     4921 cgcggcgttt agtttccttg ccgatagcgg cggctggcag cgttggttct ttgccggtat
     4981 tgcgattggt attagcgtta ccctggttgt gatgatgtat cgctcgaagg ccacgcagaa
     5041 gctaaacaat atcgcctacg cgctgattat tggcggcgcg ctgggcaacc tgttcgaccg
     5101 cctgtggcac ggcttcgttg tcgatatgat cgacttctac gtcggcgact ggcacttcgc
     5161 caccttcaac ctcgccgata ctgccatctg tgttggtgcg gcactgattg tgctggaagg
     5221 ttttttgcct tctaaagcga aaaaacaata ataaaccctg ccggataagg cctacagatt
     5281 gctgcgaaat cgtaggccgc atccggcaaa aatcctcaaa atataagagc aaacctgcat
     5341 gtctgaatct gtacagagca atagcgccgt cctggtgcac ttcacgctaa aactcgacga
     5401 tggcaccacc gccgagtcta cccgcaacaa cggtaaaccg gcgctgttcc gcctgggcga
     5461 tgcttctctt tccgaagggc tggagcaaca cctgctgggg ctgaaagtgg gcgataaaac
     5521 cactttctcg ctggagccag atgcggcgtt tggcgtgccg tcaccggacc tgattcagta
     5581 cttctcccgc cgtgagttta tggatgcagg cgagccagaa attggcgcaa taatgctttt
     5641 taccgcaatg gatggcagtg agatgcctgg cgtgatccgc gaaattaacg gcgactccat
     5701 taccgtcgat ttcaaccatc cgctggccgg gcagaccgtt cattttgata ttgaagtgct
     5761 ggaaatcgat ccggcactgg aggcgtaaca tgcagatcct gttggccaac ccacgtggtt
     5821 tttgtgccgg ggtagaccgc gctatcagca ttgttgaaaa cgcgcttgcc atttacggcg
     5881 caccgatata tgtccgtcac gaagtggtgc ataaccgcta cgtggtcgat agcctgcgcg
     5941 agcgtggagc tatctttatt gagcagatca gcgaagtgcc ggacggcgcg atcctgatct
     6001 tctccgcaca tggtgtttct caggcggtac gtaacgaagc gaaaagccgt gatttgacgg
     6061 tattcgacgc cacctgtccg ctggtgacca aagtgcatat ggaagtcgcc cgcgccagcc
     6121 gtcgtggcga agagtctatt ctcatcggtc acgccgggca cccggaagtg gaagggacga
     6181 tggggcagta cagcaaccct gaagggggaa tgtatctggt cgaatcgcct gacgatgtgt
     6241 ggaaactgac ggtcaaaaac gaagagaagc tctcctttat gacccaaacc acgctgtcgg
     6301 tagatgacac gtctgatgtg atcgacgcgc tgcgtaaacg cttcccgaaa attgtcggtc
     6361 cgcgcaaaga tgacatctgc tacgccacga ctaaccgtca ggaagcggta cgcgccctgg
     6421 cagaacaggc ggaagttgtg ttggtggtcg gttcgaaaaa ctcctccaac tccaaccgtc
     6481 tggcggagct ggcccagcgt atgggcaaac gcgcgttttt gattgacgat gcgaaagata
     6541 tccaggaaga gtgggtgaaa gaggttaaat gcgtcggcgt gactgcgggc gcatcggctc
     6601 cggatattct ggtgcagaat gtggtggcac gtttgcagca gctgggtggt ggtgaagcca
     6661 ttccgctgga aggccgtgaa gaaaatattg ttttcgaagt gccgaaagag ctgcgtgtcg
     6721 atattcgtga agtcgattaa gtcattagca gcctaagtta tgcgaaaatg ccggtcttgt
     6781 tatcggcatt ttttatggag aaaacatgcg cttacctatc ttcctcgata ctgaccccgg
     6841 cattgacgat gccgtcgcca ttgccgccgc gatttttgcc cccgaactcg acctgcaact
     6901 gatgaccacc gtcgcgggta atgtctcggt tgagaaaact acccgtaacg ccttgcaact
     6961 gctgcatttc tggaatgcgg agattccgct cgcccaggga gccgctgtgc cgctggtacg
     7021 cgcaccgcgt gatgcagcat ctgtgcacgg cgaatcggga atggctggct acgactttgt
     7081 tgagcacaac cgaaagccac tcgggatacc ggcgtttctg gcgattcggg atgccctgat
     7141 gcgtgcacca gagcccgtta ccctggtggc catcggcccg ttaaccaata ttgcgctgtt
     7201 actttcacaa tgcccggaat gtaaaccgta tattcgccgt ctggtgatca tgggtggttc
     7261 tgccggacgc ggcaactgta cgccaaacgc cgagtttaat attgctgccg atccagaagc
     7321 tgctgcctgt gtcttccgca gtggtattga aatcgtcatg tgcggtttgg atgtcaccaa
     7381 tcaggcaata ttaactcctg actatctctc tacactgccg cagttaaacc gtaccgggaa
     7441 aatgcttcac gccctgttta gccactaccg tagcggcagt atgcaaagcg gcttgcgaat
     7501 gcacgatctc tgcgccatcg cctggctggt gcgcccggac ctgttcactc tcaaaccctg
     7561 ttttgtggca gtggaaactc aaggcgaatt tacctcaggc acgacggtgg ttgatatcga
     7621 cggttgcctg ggcaaaccag ccaatgtaca ggtggcattg gatctgaatg taaaaggctt
     7681 ccaacagtgg gtggctgagg tgctggctct ggtgccgtaa cctgtcacat gttatcggca
     7741 tgcagtcatt catcgactca tgcctttcac tgatatccct ccctgtttat cattaatttc
     7801 taattatcag cgtttttggc tggcggcgta gcgatgcgct ggttactctg aaaacggtct
     7861 atgcaaatta acaaaagaga atagctatgc atgatgcaaa catccgcgtt gccatcgcgg
     7921 gagccggggg gcgtatgggc cgccagttga ttcaggcggc actggcatta gaaggcgtgc
     7981 agctgggcgc ggcgctggag cgcgaaggat cttctttact gggcagcgac gccggtgagc
     8041 tggctggtgc agggaaaaca ggcgttaccg tgcaaagcag tcttgatgcg ataaaagatg
     8101 attttgatgt gtttatcgat tttacccgtc cggaaggtac gctgaaccat ctcgcttttt
     8161 gtcgccagca tggcaaaggg atggtgatcg gcactacggg gtttgacgaa gccggaaaac
     8221 aggcaattcg tgacgccgcc gccgatattg cgattgtctt tgccgccaat tttagcgttg
     8281 gcgttaacgt catgcttaag ctgctggaga aagcagccaa agtgatgggt gactacaccg
     8341 atattgaaat tattgaagca catcatagac ataaagttga tgcgccgtca ggcaccgcac
     8401 tggcaatggg agaggcgatc gcccacgccc ttgataaaga tctgaaagat tgcgcggtct
     8461 acagtcgtga aggccatacc ggtgaacgtg tgcctggtac cattggtttt gccaccgtgc
     8521 gtgcaggtga catcgttggc gaacataccg cgatgtttgc cgatattggc gagcgtctgg
     8581 agatcaccca taaggcgtcc agccgcatga catttgctaa cggtgcggta agatcggctt
     8641 tgtggttgag tggtaaggaa aatggtcttt ttgatatgcg agatgtgctt gatctcaata
     8701 atttgtaacc acaaaatgtt tatgatgatg caaaagtaac atatttaatt tattaattat
     8761 aaagggcttt aatttttagc ccttttattt ttggtgttat gcttttaatt tttctataac
     8821 tgccaaaaat tacatgtttt g
//
LOCUS       DBFIFI010000177         8838 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.177, whole genome shotgun sequence.
ACCESSION   DBFIFI010000177 DBFIFI010000000
VERSION     DBFIFI010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8838
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.177"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..630
                     /locus_tag="N7B39_003828"
     CDS             <1..630
                     /locus_tag="N7B39_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HGU2423727.1"
                     /translation="TTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLK
                     ELNNPEHDSYAMSEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIA
                     EQKKESEMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVVNEDDCKIRRGNA
                     AELFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVFAGSGLS"
     gene            896..3133
                     /gene="nrfB"
                     /locus_tag="N7B39_003829"
     CDS             896..3133
                     /gene="nrfB"
                     /locus_tag="N7B39_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HGU2423728.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            3120..6092
                     /gene="nfrA"
                     /locus_tag="N7B39_003830"
     CDS             3120..6092
                     /gene="nfrA"
                     /locus_tag="N7B39_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HGU2423729.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSITTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAEALRLARLLYQQESANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARISQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAARQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGSNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNTQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRFFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            6093..6983
                     /locus_tag="N7B39_003831"
     CDS             6093..6983
                     /locus_tag="N7B39_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HGU2423730.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAVPGVTPDGWYFSAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEHVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            7166..7927
                     /gene="envY"
                     /locus_tag="N7B39_003832"
     CDS             7166..7927
                     /gene="envY"
                     /locus_tag="N7B39_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HGU2423731.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(7973..8049)
                     /locus_tag="N7B39_003833"
     tRNA            complement(7973..8049)
                     /locus_tag="N7B39_003833"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8013..8015),aa:Arg,seq:tct)
     gene            8292..>8838
                     /gene="fimZ"
                     /locus_tag="N7B39_003834"
     CDS             8292..>8838
                     /gene="fimZ"
                     /locus_tag="N7B39_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HGU2423732.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNSSTVTVLSNREVTILRY
                     LVSGLSNKEIADKLLLSNKTVS"
BASE COUNT         2260 a         2224 c         2250 g         2104 t
ORIGIN      
        1 acaactgatg cgatgggttg ccagaaagat attgcagaga agatacaaaa acagggaggt
       61 gattatttat tcgcggtaaa aggaaaccag gggcggctaa ataaagcctt tgaggaaaaa
      121 tttccgctga aagaattaaa taatccagag catgacagtt acgcaatgag tgaaaagagt
      181 cacggcagag aagaaatccg tcttcatatt gtttgcgatg tccctgatga acttattgat
      241 ttcacgtttg aatggaaagg gctgaagaaa ttatgcgtgg cagtctcctt tcggtccata
      301 atagcagaac aaaagaaaga gtcagaaatg acggtcagat attatatcag ttctgctgat
      361 ttaaccgcag agaagttcgc cacagcaatc cgaaaccact ggcacgtgga gaataagctg
      421 cactggcgtc tggacgtggt agtgaatgaa gacgactgca aaataagaag aggaaatgca
      481 gcagaattat tttcagggat acggcacatt gctattaata ttttgacgaa tgataaggta
      541 ttcaaggcag ggttaagacg taagatgcga aaagcagcca tggacagaaa ctatctggcg
      601 tcagtctttg cggggagcgg gctttcgtaa tcttgccctg cctgctcacc tccaggcact
      661 tccactacat catgacctga tcggtgccac cactcctctt cactatgacg ttaacctccc
      721 ctccttgaca ccacgctttt gtctacattc tacgcaaacg gcaactttta taaccagtgt
      781 aaaaataacg tgccgcaata gtcataccta ttaatggtaa aaagctgtca caattcataa
      841 aaaaccttaa tatacgccac cctaaacata accagcgtta atgtaaggtt tttgtgtgga
      901 ctggcttctt gatgtttttg ctacctggct ctatggctta aaagtaatcg cgataacgtt
      961 agcggtcatc atgttcatca gcgggctgga cgattttttt attgatgtcg tctactgggt
     1021 acgccgcatt aaacgcaagt tgagtgttta tcgccgctac ccgcgaatga gttaccgcga
     1081 actgtataaa ccagatgaaa aaccgttagc gattatggtt ccggcgtgga atgaaacggg
     1141 cgtcatcggc aatatggccg agctggcggc gaccacgctc gactacgaaa actatcatat
     1201 ctttgttggc acctacccca acgacccgga cactcagcgt gatgttgacg aagtgtgcgc
     1261 tcgcttcccg aatgtgcata aggtagtctg cgcgcgtcct ggccccacca gcaaagccga
     1321 ctgtctgaac aacgtgctgg acgccatcac ccagtttgag cgtagcgcca atttcgcttt
     1381 tgctggtttt attctgcatg acgccgaaga tgtgatttca ccgatggaat tgcgtctgtt
     1441 caactatctg gtcgagcgta aagatctgat tcagatcccg gtgtatccgt ttgaacgcga
     1501 atggacgcac ttcaccagca tgacttacat tgatgagttt tcagagctgc atggcaaaga
     1561 tgttccggtg cgtgaagccc tcgccggaca agtgcccagc gcaggcgtcg gcacctgttt
     1621 cagccgccgc gccgtgaccg ctctgttagc tgacggtgac ggtattgctt tcgacgtgca
     1681 gagtctgact gaagattacg acattggctt ccgcctgaaa gaaaaaggta tgacggaaat
     1741 ttttgtccgt tttccggtgg tggacgaagc caaagaacgc gagcagcgta aatttttaca
     1801 gcacgcacgg acgtcaaaca tgatctgcgt gcgcgaatat ttccccgata ccttttcgac
     1861 tgcggttcga caaaaatctc gctggatcat cggcattgtt ttccagggct ttaaaaccca
     1921 taaatggacc tccagcctga cgctgaacta ctttctctgg cgcgaccgca aaggggcaat
     1981 cagtaacttt gtcagcttcc tcgcaatgct ggtgatgatc cagcttttgc tgttgctggc
     2041 gtatgaaagt ttgtggcccg atgcctggca tttcctttct atttttagcg gcagcgcatg
     2101 gttaatgacc ctgctgtggc taaactttgg cttgatggtt aaccgcatcg tgcagcgggt
     2161 gattttcgtc actggctact acggcctgac gcaggggcta ctatctgtcc tgcgtctttt
     2221 ctggggcaac ctgattaact ttatggccaa ctggcgcgcg ttaaaacagg tacttcaaca
     2281 cggcgatcca cgtcgcgtcg cgtgggataa aacaacgcat gacttcccca gcgtcactgg
     2341 cgatacccgc tcgttgcgcc cgttaggtca aattctgctg gaaaatcagg tcatcactga
     2401 agaacagctc gatacagcac tgcgtaatcg cgtcgaaggt ctacgcctgg gcggttcaat
     2461 gctgatgcag gggctgatta gcgccgagca gctggcacag gcgctggcag agcaaaacgg
     2521 cgtggcgtgg gaatccatcg atgcctggca gatcccgtcc tcgctgattg ccgaaatgcc
     2581 ggcctccgtg gcgctgcatt atgcggtact gccgctgcgt ctggaaaatg atgagttaat
     2641 tgtcggcagt gaagatggta ttgacccggt ttcgctggcg gccctgacgc gtaaagtcgg
     2701 acgcaaagtg cgttatgtca ttgttctgcg gggacaaatt gtcacggggt tacgtcactg
     2761 gtatgcacgc cgacgcggtc acgatccgcg ggcaatgttg tacaatgcgg ttcagcatca
     2821 gtggctcacg gaacagcagg ccggtgaaat ctggcggcaa tatgtgccgc atcagttcct
     2881 gttcgccgaa atactgacca cgctcggtca tattaatcgt tcagcaatta acgtgttgtt
     2941 attgcgccat gaacgcagtt ctctgccgct cggcaagttt ttggtcaccg aaggcgttat
     3001 cagccaggaa acgttggatc gcgtcctgac aattcaacgc gaattacaag tttcgatgca
     3061 atcactatta ctcaaagcag gtttaaacac agaacaggtt gcgcaactgg agtccgaaaa
     3121 tgaaggagaa taaccttaat cgcgtcatcg gatggtctgg tttactgctg acgtctttat
     3181 tgagtaccag cgcactcgca gacaatatcg gcaccagcgc agaagagctg gggctgagcg
     3241 attatcgcca ttttgttatt tatccccgtc tcgataaggc gctgaaggca cagaaaaata
     3301 acgacgaagc aaccgccatc cgcgaatttg aatatataca ccagcaggtg ccggataata
     3361 ttccgctgac tttatacctt gcggaagcct atcgccattt tggtcatgat gaccgggcac
     3421 ggctgttgct tgaggatcaa ctgaaacgtc acccaggaga tgcccgactt gagcgcagtc
     3481 tggcggctat tccggttgaa gtgaaaagca ttacaactgt tgaagaactg cttgcccaac
     3541 aaaaagcgtg cgatgctgcg ccgaccctgc gttgtcgcag tgaagtcggg cagaatgccc
     3601 tgcggctggc acagttacct gtcgccagag cgcaactgaa cgacgcgacg tttgctgcat
     3661 cgccggaagg aaaaacgctg cgaaccgatc tgctgcaacg ggcaatctac ctgaaacaat
     3721 ggtcccaggc agatacgcta tacaatgaag cacgccagca gaacacatta agcgcggcag
     3781 aacgccgtca gtggtttgac gtgcttcttg ccgggcagct ggacgatcgg atcctggcac
     3841 tgcaatcaca ggggatcttc accgatcctc agtcatatat tacttacgcg accgcgctgg
     3901 cttatcgtgg cgaaaaagca cgcctccagc attatctcat tgaaaataag ccgctgttta
     3961 ccacggacgc acaagagaaa agttggctct atctgttatc taaatacagc gccaaccccg
     4021 ttcaggcatt ggcgaattat acggtgcagt ttgccgataa ccgccagtat gttgttggcg
     4081 cgacgctacc ggtgctgtta aaagaaggtc agtacgacgc agcgcaaaaa ctgctcgcca
     4141 ccctccccgc caatgaaatg cttgaggagc gttatgctgt cagcgtggcg acccgtaaca
     4201 aggctgaagc tctgcgtctg gcacgcttgc tgtatcagca agaatcggca aatcttaccc
     4261 gcctggatca actaacctgg caactgatgc agaacgagca gtcacgggaa gctgccgatt
     4321 tattgctgca acgctatcct ttccagggcg atgcgcgtat cagccagact ttaatggcgc
     4381 gactggcgtc tctgctggaa agtcatcctt acctggcaac gccggcgaag gtggcgattt
     4441 tatcgaaacc cttaccgctg gcggagcaac gtcagtggca aagtcagttg ccgggtattg
     4501 cagataattg cccggcaata gttcgcttgc tgggcgatat gtcgccttcc tacgatgccg
     4561 ccgcctggaa ccgtctggca aagtgttatc gggacacgct acccggtgtg gcgttgtatg
     4621 catggcttca ggccgaacaa cgacaaccga acgcctggca acatcgtgcg gtagcctatc
     4681 aggcgtatca ggttgaggac tacgccaccg cactggcggc caggcagaaa atcagtcttc
     4741 acgacatgag caatgaggat ctgcttgctg ctgccaatac cgcccaggcg gcaggaaatg
     4801 gtgcggctcg cgatcgctgg ctacaacagg cagaacaacg tggactggga agcaatgccc
     4861 tctactggtg gctgcatgcg caacgttaca ttcctggtca gccggaactc gcactgaacg
     4921 atctcacgcg ctcaatcaat attgcgcctt ctgccaacgc ttacgttgcg cgggcgacaa
     4981 tttatcgcca acgtcataat gtcccggccg cggtgagtga tttgcgcgcc gcgctggaac
     5041 tggaaccgaa taatagcaac acccaggcag cgcttggtta cgccttgtgg gatagcggtg
     5101 atatcgcaca gtcgcgggaa atgctcgaac aggcgcataa agggctaccg gacgatccgg
     5161 cactgatccg acaactggcc tatgtgaacc agcgtctgga tgacatgcct gcgacgcagc
     5221 actacgcccg gctggtgatt gatgacattg ataatcaggc gctgataacc ccactgaccc
     5281 cagagcaaaa tcagcaacgc ttcaatttcc gccgtctgca tgaggaggtc ggtcgccgct
     5341 ggacgttcag tttcgattct tccatcggct tgcgttccgg ggcaatgagt accgctaaca
     5401 ataatgttgg cggcgcagcg ccagggaaaa gctatcgtag ctacgggcaa ctggaagccg
     5461 aataccgcat cggacgcaat atgctgctgg aaggcgacct gctctcagtt tatagccgct
     5521 tctttgccga taccggagaa aacggggtga tgatgccggt gaaaaatccg atgtccggca
     5581 ccggtctgcg ctggaagccg ctgcgcgatc agatcttttt cctcgccgtc gaacagcagt
     5641 tgccgctgaa cgggcaaaat ggcgcatccg ataccatgct gcgcgccagc gcctcattct
     5701 ttaatggcgg caaatacagc gacgaatggc acccgaacgg ttcaggctgg tttgcccaaa
     5761 acctgtacct cgatgcggcg caatatatcc gccaggatat tcaggcgtgg acggcagatt
     5821 atcgcgtcag ctggcatcag aaggtagcta acggacagac tattgagcct tacgctcacg
     5881 ttcaggacaa cggctatcgt gataaaggca ctcagggcgc gcagcttggc ggtgtcgggg
     5941 tccgctggaa tatctggacc ggcgagacgc actacgacgc ctggccgcac aaagtcagtc
     6001 tcggcgtcga gtatcaacat acctttaagg cgattaatca acgtaacgga gagcgcaaca
     6061 acgcgtttct caccattgga gtgcactggt aaatgcgtaa gttcattttc gtattgctga
     6121 cactgctttt ggtcagccct ttttcctttg cgatgaaagg tattatctgg caaccacaaa
     6181 accgtgatag tcaggttacc gatacccagt ggcaggggct gatgagtcag ttacgtttgc
     6241 aaggcttcga taccctggtt ttgcaatgga cccgttacgg cgatgcattc acccagccag
     6301 aacagcgcgc gttattgttt aagcgggccg cagctgcgca acaggctggc ctgaagctga
     6361 ttgtcgggct gaacgccgat ccggaatttt ttatgcacca gaaacagtca tccgcggcgc
     6421 tggaaagcta tcttaatcgc ctgctggctg ccgatctcca gcaagccaga ttatggagtg
     6481 ccgtacctgg cgtaacgccg gatggctggt acttcagcgc tgaaattgac gacctgaact
     6541 ggcgcagcga agccgcccgt cagcctttgc taacatggtt aaacaacgcg cagcggctga
     6601 ttagcgatgt ttcagcaaaa ccggtttata tcagtagttt tttcgccgga aacatgtcgc
     6661 cagatggcta tcgccaactg ctggaacacg ttaaagcaac cggcgttaat gtctgggtac
     6721 aggatggcag cggcgtggat aaacttaccg ctgaacagcg tgaacgttat ctacaggcca
     6781 gcgccgattg ccaaagctcc gccccagcaa gcggcattgt ttatgaactg tttgtcgccg
     6841 gcaaaggcaa aacctttaca gcgaaaccga aaccggacgc agaaattgcc tcgctgttag
     6901 cgaaacgttc ctcttgcggt aaagacactc tctatttctc tctgcgctat ttgcccgtcg
     6961 cgcacggcat tctcgagtat taaatctcct ccaggtaagt cgggtacgac ctggcttacc
     7021 tctttcgctc tcgtcagaat tccatcaaga ttaattgcta aaaagcgtgc taaagaaaga
     7081 agatcgttat cggcgaacgg gccgatcact catgcaggaa gcatagatcc tttgagcgga
     7141 gtttctgata tctgaggagt gcgaaatgca attgagcagc agtgaacctt gcgtggtgat
     7201 cctgaccgaa aaagaggtag aggtaagcgt caataaccat gctacgttta cccttccgaa
     7261 aaactacctg gccgccttcg cgtgcaacaa taacgtcatt gaactctcaa cgttaaatca
     7321 cgtattaatc acccacatca accgtaacat catcaacgat tatctgttgt ttttaaataa
     7381 gaacttaacc tgtgtaaagc cctggtcgcg gctggctacc ccggttatcg cttgtcatag
     7441 ccgtacaccg gaagtgttcc ggctagccgc caaccacagc aagcagcaac ccagcaaacc
     7501 ctgcgaagcg gagttgacgc gcgcattgct ttttaccgta ttgtctaact ttcttgagca
     7561 atcgcggttt attgccctac tgatgtatat cttacgcagc agcgtccgcg acagcgtctg
     7621 ccgcattatt caaagcgata ttcagcatta ctggaatctg cgaattgtcg ccagttcgct
     7681 atgtttaagc cccagcctgc tcaaaaagaa attaaaaaac gaaaatacca gctatagcca
     7741 gattgtcaca gagtgtcgta tgcgttacgc cgtacagatg ttattgatgg ataacaaaaa
     7801 tatcactcag gtggcgcaat tatgtggcta tagcagcacg tcgtacttta tctctgtttt
     7861 taaggcgttt tacggcctga caccgttgaa ttatctcgcc aaacagcgac aaaaagtgat
     7921 gtggtgaagg gcaaagcgga aacggataag acgggcataa ataaagaaga aatggcgcgc
     7981 cctgcaggat tcgaacctgc ggcccacgac ttagaaggtc gttgctctat ccaactgagc
     8041 taagggcgcg ttgataccgc aatgcggtgt aatcgcgtga attatacggt caacccttgc
     8101 tgagtcaatg gcttttgatc tggttgctga acaagtgaac gaccgcgtct gattttctga
     8161 tttatttcgc tatagcggca aacaaacgca caccgctgcg cgtctgaatc aagaaaaccc
     8221 gtattttcat gtatcaaagt acaatttacc gccctaacgg aaaattgtcc gctcctatga
     8281 gactggtaac tatgaaacca acgtcggtga tcattatgga cactcatcct atcatcagaa
     8341 tgtctattga agttctgttg caaaaaaaca gtgaattgca gattgtcctg aaaacggatg
     8401 attaccgcat aaccatcgat tatctccgaa cccgtcctgt tgatttaatc attatggata
     8461 tagacttgcc cggaacagac ggttttacct tcctgaaaag gatcaaacaa atccagagca
     8521 cagtgaaagt gttattttta tcatcgaaat cagaatgctt ttatgctggc agagcgatac
     8581 aagctggtgc taacggtttt gtcagtaaat gcaatgatca gaatgatatt tttcatgccg
     8641 ttcagatgat cctctccgga tacacgtttt ttcccagcga aacgcttaac tatataaaaa
     8701 gcaataaatg tagtacgaat agttcaacgg tcactgtgct atctaatcgt gaagtgacca
     8761 tattacgtta tctagttagc ggattatcta ataaagaaat tgccgataag ttattactta
     8821 gcaataaaac agttagtg
//
LOCUS       DBFIFI010000178         8670 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.178, whole genome shotgun sequence.
ACCESSION   DBFIFI010000178 DBFIFI010000000
VERSION     DBFIFI010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8670
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.178"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..164)
                     /locus_tag="N7B39_003835"
     CDS             complement(<1..164)
                     /locus_tag="N7B39_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HGU2423733.1"
                     /translation="MKTISVESLSIISFSNIPVVTTELLASLYGTEPDYIRKNFNRNS
                     GRFVIGKHYF"
     gene            complement(420..1178)
                     /locus_tag="N7B39_003836"
     CDS             complement(420..1178)
                     /locus_tag="N7B39_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HGU2423734.1"
                     /translation="MTTKISVETLSPITHNQIPVITTELLAQLYGTEPVRIRQNHHEN
                     KVRFVEGKHFFKVVGNDLKELRVALNYSQNLRVTLSNSQNLRVTLSNSQNLQPSLRGL
                     QISPKARSLILWTERGAARHAKMLETDRAWEVFEKLEDCYFSQKIPEQLQLPESTLSI
                     NYPLSWFSEHHPYSMMSYVDRKTLNLDVSVLFDMPSPTMRILNELHSKGYNVDAAVAE
                     FNAFKHLTEEMRRTLQDISRLSDRNSRKGFSLSL"
     gene            complement(1453..1635)
                     /locus_tag="N7B39_003837"
     CDS             complement(1453..1635)
                     /locus_tag="N7B39_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HGU2423735.1"
                     /translation="MRRQRRSITDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARKTR"
     gene            complement(1632..2159)
                     /locus_tag="N7B39_003838"
     CDS             complement(1632..2159)
                     /locus_tag="N7B39_003838"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HGU2423736.1"
                     /translation="MTIKSNTPAHDKDCWQTPLWLFDALDIEFGFWLDSAASDKNALC
                     AHWLTEADDALNSEWISHGAIWNNPPYSNIRPWVEKAAEQCIQQRQTVVMLVPEDMSV
                     GWFSKALESVDEVRIITDGRINFIEPSTGLEKKGNSKGSMLLIWRPFISPRRMFTTVS
                     KAALMAIGLGVRRAA"
     gene            complement(2156..2602)
                     /locus_tag="N7B39_003839"
     CDS             complement(2156..2602)
                     /locus_tag="N7B39_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HGU2423737.1"
                     /translation="MKQTFLLRNEAIRNNAIDAILSLPIDDKSPHEVHVKEPKRSKAQ
                     NDRMWPMLNDVSRQVLWHGQRLAPEDWKDLFTALWLKTKKLEQRSAPGIDGGVVMLGV
                     RTSKMRKASMTELIEIMFWFGSERNVRWSDDSRREYEWSQRKGKAA"
     gene            complement(2559..2795)
                     /locus_tag="N7B39_003840"
     CDS             complement(2559..2795)
                     /locus_tag="N7B39_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lar family restriction alleviation protein"
                     /protein_id="HGU2423738.1"
                     /translation="MSDLSLTQPKLKECPFCGGNARLWVEAGINIDVWGYAECDLCEA
                     RVAWAPSVAAATEKWNRRAGDEANLSASQRSNQK"
     gene            complement(2806..3021)
                     /locus_tag="N7B39_003841"
     CDS             complement(2806..3021)
                     /locus_tag="N7B39_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2423739.1"
                     /translation="MSTIAELVRANFREELVRWYRYRSSSSLPLDELYEHSPAARRYP
                     RDRVLRRLFKLNNEFQRNRIIRSLDFK"
     gene            complement(3154..3432)
                     /locus_tag="N7B39_003842"
     CDS             complement(3154..3432)
                     /locus_tag="N7B39_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2423740.1"
                     /translation="MNDSYRQFENWWSKDKSQFTGDDELKEFAWVIWQASRSAIELDI
                     DWPESNDDLWKDGEEGAYAMGYEDGRDKTVIAVMKAIRAAGIKEKNFD"
     gene            complement(3503..4879)
                     /locus_tag="N7B39_003843"
     CDS             complement(3503..4879)
                     /locus_tag="N7B39_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HGU2423741.1"
                     /translation="MRQDIEASVIGGLLIGGLTPTASDVLATLEPEAFSIPLYRKAFE
                     VIRKQARNRNLIDALMVAEACGEEHFTSILMTSKNCPSAANLKGYAGMVADNYHRRLV
                     LEIMDEMREPIQSGTIDASSQAMDELVKRLSAIRKPRDEVKPVRLGEIITDYTDTLDR
                     RLRNGEESDTLKTGIEELDAITGGMNAEDLVIIAARPGMGKTELALKIAEGVASRVIP
                     GSDVRRGVLIFSMEMSALQIAERSIANAGRMSVSVLRNPASMDDEGWARVANGMSQLA
                     DLDVWVVDASRLSVEEIRSIAERHKQENPNLSLIMADYLGLIEKPKADRNDLAIAHIS
                     GSLKAMAKDLKTPVISLSQLSRDVEKRPNKRPTNADLRDSGSIEQDADSIIMLYREAV
                     YDENSSAAPFAEIIVTKNRFGSLGTVYQRFCNGHFVACDQDEARQICTASNAPAARGR
                     RYAQGADV"
     gene            complement(4876..5697)
                     /locus_tag="N7B39_003844"
     CDS             complement(4876..5697)
                     /locus_tag="N7B39_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HGU2423742.1"
                     /translation="MGVVKLADYRPQLEVVEHRVADTEDGFMRVANEITDSLLMADLT
                     VRQLKVMLAIMRKTYGFNKPMDRLTNTQIAAMTGIHHTHVCAAKRQLIERKFLIADGV
                     KIGVNKVVSQWISQDSLTLAKTANKTLAKSANGYKPSQLNTKDNIQKTINTNTPLPPN
                     GDGDGQVKPERRKAERIDYESFLNAYNTEVGDRLPHAVAVNEKRKRRLKKIIPQLKTP
                     NVDGFRAYVRAFVHQAKPFYFGDNDTGWTADFDYLLREDSLTGVREGKFADRGIA"
     gene            complement(5684..5845)
                     /locus_tag="N7B39_003845"
     CDS             complement(5684..5845)
                     /locus_tag="N7B39_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2423743.1"
                     /translation="MTKRRKKYQEKEEIRHPDSPEGLVVAAANNRAFAERLVGVYRLA
                     KAGVKHGRR"
     gene            complement(5878..6174)
                     /locus_tag="N7B39_003846"
     CDS             complement(5878..6174)
                     /locus_tag="N7B39_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HGU2423744.1"
                     /translation="MERTSYSKLSQRDVDRAETDLLINLSTLTQRGLAKMIGCHESKI
                     SRTDWRFIASVLCAFGMASDISPISRAFKYALDEITKKKSPAATEDFKQIDMQF"
     gene            complement(6316..6531)
                     /locus_tag="N7B39_003847"
     CDS             complement(6316..6531)
                     /locus_tag="N7B39_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU2423745.1"
                     /translation="MSNLRKYRESLNISQTTLAKAVGCTQGAIGHWESGRRFPDLKTC
                     RALVACLNKLGAKVSLDDVFPPEHKAA"
     gene            6607..7302
                     /locus_tag="N7B39_003848"
     CDS             6607..7302
                     /locus_tag="N7B39_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HGU2423746.1"
                     /translation="MDGSSTERNNKDMKMKWYELARSRMKELGITQEKLAEELGMTQG
                     GIGHWLRGSRHPSLSDIGVVFKYLGIDNISFNHDGTFSPVGEYSSAPVKKQYEYPVFS
                     HVQAGMFSPELRTFTKGDAERLVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPDGML
                     ILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSVVG
                     KVIASQWPEETFG"
     gene            7804..8325
                     /locus_tag="N7B39_003849"
     CDS             7804..8325
                     /locus_tag="N7B39_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2423747.1"
                     /translation="MAKSNVSVQAFKDFLEELMSLNIMKEATARNLKNSSARLLTVVQ
                     EEEMGDVTQLDVNELAERYINATEPKPSDSSITAYKSRMESAIKKFVAFQSGEEIPYT
                     PIDKESSEEKDLTGEPTKVEGKANALHTYDLPVVLRPESGVTVTIKGIPNDITNEEAE
                     RISSILKVYVRPQ"
BASE COUNT         2145 a         2142 c         1998 g         2385 t
ORIGIN      
        1 aaaaagtagt gcttaccgat aacaaatcgt ccagaattcc gattgaaatt ttttcggatg
       61 taatctggtt ctgtgccata taagctggca agaagctctg tagtcaccac aggtatatta
      121 ctaaatgaaa ttatggagag ggactccacg gagatggttt tcatgcttta tgtcctactt
      181 gatattttga tttacccctt ttgagagggt gaccgggcgc tcaaaaccgc aagtagtcgg
      241 cgggcatatt cccattgctg gtgttgtatt agccgcacgc ccggtcataa accaagaaga
      301 ctggacacaa aaaaaccgca tgtctgtcgg gcgcggtaac cgctacttgc taaggtgttt
      361 tgagcaccat gaggcggaat atatccccgt taatgcagca ttgtcaaacg atctgttctt
      421 taaagactta acgagaagcc ttttcgggaa tttcgatctg acaatcttga aatatcctgt
      481 agagtacggc gcatttcttc cgtcagatgc ttgaaggcgt taaattcggc gacagctgca
      541 tcgacgttat agcccttgct gtgtagctcg ttgaggatgc gcatagttgg gcttggcata
      601 tcgaagagca cagaaacgtc caggttaaga gtttttcgat caacatagct catcatggag
      661 taggggtgat gctctgaaaa ccacgagaga gggtagttga tagatagtgt tgattctgga
      721 agctgaagtt gttctgggat tttttgactg aaatagcagt cttccagttt ttcgaacact
      781 tcccacgccc gatcggtttc gagcattttt gcgtgacggg ctgcgccgcg ttctgtccag
      841 aggatgaggg agcgggcttt cggggaaatt tgtaaccctc ttaaagatgg ttgcaaattt
      901 tgtgagttac ttaaagtaac ccgcaaattt tgtgagttac ttaaagtaac ccgcaaattt
      961 tgtgagtagt ttaaagctac ccgcaattct ttaaggtcat taccaacaac tttgaaaaag
     1021 tgtttccctt caacgaagcg tactttgttc tcatgatgat tctggcgaat acgcaccggc
     1081 tcagtgccgt aaagctgcgc caaaagttcg gtggtaataa caggaatctg gttatgggtg
     1141 atcggggaga gagtttcaac agaaatttta gttgtcataa tgacgccctc tggtggtttc
     1201 ttaataactc accaccgacg acgccaatcg tctggtggtg aactgtgcag ggttggcgta
     1261 accgggaaac cgaccggcgc ggatctctcc gcccccacac agcccaccat aattcagatg
     1321 tgcgcgtgca tacgacaata aaaaacacgc tcgcggcgtg tatctgtcgc ggtctctatc
     1381 caggacgcca atcccgacgc cagattttgc tggcgtttga ggaatatagc cccgaataaa
     1441 tcatcacgtc aatcaccttg ttttcctcgc acgatgtctt agccaccgga tatcccacag
     1501 gtgagccgtg taattgaagg tttttacgtc agattctttt gggattggct tgcgtttatt
     1561 tctggagcgt ttcgttggaa ggtatttgca gttttcgcag atgatgtcgg tgatacttcg
     1621 tcgctgtcgc ctcatgcagc cctcctgacg cccagcccga tcgccatcaa tgccgctttg
     1681 gatacggtag taaacatccg tcgaggactg atgaacggtc gccaaatcag caacattgag
     1741 cctttgctgt ttcccttctt ctccagccct gtcgatggtt cgataaaatt aatccgtcca
     1801 tcagtgatga tgcgaacttc gtcaacactc tccagagcct tgctgaacca tccgacagac
     1861 atatcctctg gcacaagcat cactaccgtc tgtcgctgtt gtatgcactg ctcagcggct
     1921 ttttccaccc acggcctgat attgctgtac ggtgggttat tccagattgc accgtggctt
     1981 atccactcag aatttagcgc gtcgtcagcc tcagttagcc agtgagcgca cagagcgttt
     2041 ttgtcgctcg cagctgaatc cagccagaat ccaaactcaa tatccagcgc atcaaaaagc
     2101 cagagcggcg tttgccagca gtccttgtcg tgtgctggcg tatttgattt gatagtcatg
     2161 cagccttccc ttttcgttgt gaccattcat actctcgccg ggagtcatca ctccaccgca
     2221 cgttgcgctc tgagccgaac cagaacatga tttcgataag ctcagtcatg ctggcctttc
     2281 gcattttgct ggtacgcacg ccaagcatga caacgccacc gtcgatacca ggcgcacttc
     2341 tttgctccag ttttttggtc ttaagccaca gggcagtgaa caggtctttc cagtcttccg
     2401 gcgccagccg ttgaccatgc catagcacct gacgcgaaac atcgttcagc atcggccaca
     2461 tgcggtcatt ctgcgctttg ctgcgcttgg gttctttaac gtggacttcg tggggtgact
     2521 tgtcgtcgat gggtagtgag agaatggcgt ctatggcgtt atttctgatt gcttcgttgc
     2581 gaagcagaaa ggtttgcttc atctcctgct ctccggttcc atttttcagt agccgcagca
     2641 actgatggtg cccatgccac cctggcttca cagaggtcac attctgcata gccccacaca
     2701 tcaatattta ttccggcctc aacccacaga cgagcattac cgccgcaaaa cggacattct
     2761 tttagctttg gctgggttaa tgataggtcg ctcatgctca ctccttcact taaaatccag
     2821 actccggata attctgttgc gctgaaactc attgttgagt ttgaacaacc gtcgaagaac
     2881 acggtcacgc ggatagcgtc gtgcggcagg tgaatgctca tacaactcat caagcggcaa
     2941 actggacgat gaacgatacc gataccaacg caccaactct tcacgaaaat tagccctgac
     3001 aagctcagct atcgtactca tttcttaaaa cctcctcaaa cgcattctga cgcatttttc
     3061 attctcgctg cttatcggta taccttgcac gtgcttacct caccacagag cgattgtgat
     3121 gccttaaaag cgatttattg aagtgatatt tgcttaatcg aaattctttt ctttgattcc
     3181 tgcggccctg atggctttca ttactgcaat taccgttttg tcacgcccat cctcataacc
     3241 catcgcataa gcaccttctt caccatcttt ccaaaggtcg tcattcgatt cgggccagtc
     3301 gatatccagt tcaatagcag agcgcgatgc ctgccatatc acccaggcaa actcttttaa
     3361 ttcatcgtct cccgtgaact ggcttttgtc ttttgaccac cagttttcaa actgtcggta
     3421 gctatcgttc acttccctct cccccaaata aaaaggcctg cgattaccag caggcctgtt
     3481 attaactcag tgatgtagat ggtcatacgt cagccccttg tgcatatcgt ctgccacgcg
     3541 cagcaggtgc atttgatgct gtgcaaatct gtctggcttc atcctggtca catgcaacaa
     3601 agtgtccgtt acagaaccgc tggtaaactg taccaagcga gccaaaacgg tttttcgtca
     3661 cgatgatttc agcaaatggc gcggcgctac tgttctcatc atataccgct tcccgataga
     3721 gcatgatgat tgagtctgcg tcctgttcaa tgcttcctga atcacgcaaa tctgcgtttg
     3781 tcgggcgttt gtttggtcgc ttctcaacat cgcgcgaaag ctgactcagg gagataaccg
     3841 gtgttttcag gtctttcgcc atcgccttca ggcttccgga gatgtgagca attgcgaggt
     3901 cgttgcggtc tgctttcggc ttctcaatca ggccaagata atccgccatg atgagtgaca
     3961 ggtttggatt ttcctgctta tgccgttctg cgattgagcg tatttcttcg accgataacc
     4021 gcgaagcatc gactacccat acatccaaat ctgcaagctg actcatgccg ttagcaacac
     4081 gtgcccagcc ctcgtcatcc atcgatgcag gatttcgcag tacgctaacc gacatcctcc
     4141 cggcgttggc aatgcttcgc tctgcaatct gcaatgcgct catttccatt gagaaaatca
     4201 ataccccgcg ccggacgtca gaaccaggaa taacgcggct tgccacacct tcggcaatct
     4261 tcagcgccag ttcggttttc cccataccag gacgagcggc gataatcacc aggtcttctg
     4321 cgttcatccc tccagtgata gcgtcaagct cttcgattcc ggtcttcagg gtatctgact
     4381 cttctccgtt cctcagacgc ctgtcaagcg tgtcagtgta gtcagtgatg atttccccta
     4441 accgtacagg tttaacctcg tcacggggct ttctgatggc tgaaagacgc tttacaagtt
     4501 catccatcgc ctgactcgat gcgtcgatgg ttccgctttg gattggttca cgcatttcat
     4561 ccatgatttc cagcaccaga cggcggtgat agttatccgc gaccattccg gcatatccct
     4621 tcaggtttgc ggcactcggg cagtttttgc tggtcatcag gattgacgtg aaatgctcct
     4681 ctccgcacgc ctcggcaacc atcagcgcat cgattaggtt tctgtttctc gcctgcttgc
     4741 gaataacctc gaaggctttc cggtagagcg gaattgaaaa cgcttccggc tccagcgttg
     4801 ccagaacgtc actggcggtt ggagttaatc caccaatcag caggccaccg ataacgctag
     4861 cttcgatatc ctgtctcatg caatccccct gtctgcaaac ttcccttccc gaactcccgt
     4921 taacgaatct tccctcagca ggtaatcaaa atctgccgtc cagcccgtgt cgttgtctcc
     4981 gaagtaaaac ggcttggcct gatgcacaaa cgccctgaca tacgctctga aaccgtccac
     5041 gtttggcgtt ttcagttgcg ggataatttt cttcaggcgg cgttttcgtt tctcgttgac
     5101 cgcaacagcg tgtggaagtc tgtcaccgac ttcggtgttg taggcgttca ggaaggattc
     5161 gtagtcgatt cgttctgcct tgcgacgttc aggtttaacc tgcccatcgc catccccgtt
     5221 agggggtaag ggggtatttg tatttattgt cttttgtata ttgtcttttg tgtttagctg
     5281 acttggctta tacccattag ccgacttggc taatgtttta ttagctgttt tagctaatgt
     5341 taagctgtcc tggctaatcc actgagaaac caccttgttc actccgattt tcacgccatc
     5401 agcaatgagg aatttacgct caataagctg gcgcttggca gcgcaaacat gagtgtgatg
     5461 aatacctgtc atggctgcta tctgcgtgtt tgtgagtcga tccatcggct tattgaatcc
     5521 gtatgtcttg cgcatgatag cgagcatcac cttcaactgc cggacggtta aatcagccat
     5581 cagcagactg tcggtaatct cgttagcaac gcgcatgaaa ccatcttcgg tatctgccac
     5641 gcgatgctcc acgacctcca gttgaggcct gtaatcagct aacttaacga cgcccatgtt
     5701 tcactcctgc tttggctagt ctgtaaacac caacaaggcg ctctgcgaac gccctgttat
     5761 ttgctgcggc taccactaat ccctcaggtg aatcagggtg tcgaatctct tctttttcct
     5821 ggtatttctt acgacgtttt gtcataatta ctcctgtgga ttgatccagt aattccctca
     5881 gaattgcata tcaatttgct taaaatcctc ggtggcagcc ggggattttt tctttgtgat
     5941 ttcatcaagc gcatacttaa aagccctgct aatcggactg atgtctgatg ccattccgaa
     6001 agcacacaag accgaagcaa taaaccgcca gtccgttctg cttatcttcg attcatgaca
     6061 gccaatcatc tttgccagac cgcgctgtgt aagcgttgac aggttgatga gtaaatctgt
     6121 ttctgcgcga tcaacgtcgc gctgtgatag tttgctgtaa cttgttcgtt ccatttctta
     6181 agatttccaa tagtgaatag ttagttgaaa ggtatgcgtg gaaacgcata tggccttagt
     6241 tggtcagata tcttgggact cgcttttcag cgacgtagga cgaatgtccg ttgttacaaa
     6301 gagcggctcc gcttattaag cggctttgtg ctccggcggg aacacgtcat caagacttac
     6361 ttttgcgcct aacttgttta gacacgcaac aagagcacgg catgttttaa ggtctgggaa
     6421 gcgacgacca gattcccaat gtccgatagc tccctgtgtg catccaactg ccttagcaag
     6481 tgttgtttga gagatattca gtgactctcg atattttcgt aggttgctca tatgccctcc
     6541 atagtaacca cgaataaaaa aatacaatat gtacttctca aatacaagta aaaatacaca
     6601 ttgtgcatgg atggttccag tacagagcgt aataataagg acatgaaaat gaaatggtat
     6661 gaactggcta gatccagaat gaaagagctc ggcataactc aagagaagtt agccgaagag
     6721 ctaggtatga cgcagggtgg aattggacac tggttgcgcg gatctcgtca tccatctctt
     6781 agtgatattg gtgtggtgtt taaatacctt ggtattgata acatatcatt caaccacgac
     6841 gggacatttt cacctgttgg cgaatactca tcggccccag ttaaaaaaca atatgagtac
     6901 cctgtttttt ctcatgttca ggctgggatg ttctctccag aactcagaac ctttaccaaa
     6961 ggtgatgcgg agagattggt aagcacaacc aaaaaagcca gtgactctgc attctggctt
     7021 gaggttgaag gtaactcaat gaccgcacca acaggttcca aacctagttt tcctgacggg
     7081 atgttaattc tggttgaccc tgagcaagct gttgagcccg gcgatttctg catagccaga
     7141 cttggtggtg atgaatttac cttcaagaaa ctgatcaggg atagcggtca ggtgtttcta
     7201 caaccactaa acccgcaata tccaatgatc ccatgcaatg agagttgttc cgttgtgggg
     7261 aaagttatcg ccagccaatg gcctgaagag acgtttgggt aaagaggata gatggagttt
     7321 aatggccatg agtatcaagc ggtcaaaaag aagttcatct attcattgaa agcataaggc
     7381 catctgagta tatccgtaat gatctggata ttgtctataa catcaatgac cggacgatag
     7441 atatcggtga gcagcgtcct gtatggcagg gtgaggcagg tgaaaaaacg tcctgccatc
     7501 aacaagaatc aagtgcatcc gctctctgga cagatggaaa atttattgga tgcaaaaaga
     7561 tatgaaataa cacctgtata gcacggagct ttctctgacc gatgcacttg aagttgttag
     7621 ggctgaccca gcctgctgtt tctttaggta agagaaaaga gatttaggag atgaaaggtc
     7681 gcagaggtgc ggcctttttt attgagagtg gatcttgaac ggaaattctc aagacttgag
     7741 tcttgcatgc aaatcaattc ctggataaac tcgatctgag tcaataactt agagagagag
     7801 caaatggcaa agtcaaacgt tagcgtgcag gcattcaagg acttccttga agagcttatg
     7861 tcgctgaaca taatgaagga ggccaccgct cgaaatttaa aaaactcatc cgctcgcctc
     7921 ttaacggtag tccaagaaga ggaaatgggt gatgttactc agcttgatgt gaatgagctt
     7981 gccgagcgat acatcaacgc aactgagccg aagcctagcg acagcagcat tactgcatat
     8041 aaaagccgca tggaaagtgc aatcaaaaag tttgtagctt tccagtctgg tgaagaaatc
     8101 ccatacactc cgattgacaa agaatccagt gaggaaaaag atttgactgg cgaaccaaca
     8161 aaagtcgaag gcaaggctaa tgcacttcat acctatgatc ttccagtagt tcttcgacct
     8221 gaatcagggg ttacagtaac gattaaaggc attcctaacg atatcacaaa cgaagaagcc
     8281 gaacgcatct cttcaattct gaaggtttac gttcggcctc aataatgcaa aagcattcag
     8341 cacaatgtcc aactccccag tccgacattg atgctgttta accagagcct ccaaaaggag
     8401 cccttgttaa ggtacacaat atttgcgatg taaccttagc gcgtctggta cattttttca
     8461 agcggttgta gggctgccgc caatatgaga aaacacagat gtctacatac aatttgaatg
     8521 accaattcga tcgagaagtt catgtgaatg cctatgaacg gataaggcat ggaaatcttg
     8581 aacatgtgtg tgagcattat cgctcaaggc cacaccgcta atcatcaacc cggcctccat
     8641 gccgggtttt cttttcctct cgcccaaaaa
//
LOCUS       DBFIFI010000179         8663 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.179, whole genome shotgun sequence.
ACCESSION   DBFIFI010000179 DBFIFI010000000
VERSION     DBFIFI010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.179"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            135..479
                     /locus_tag="N7B39_003850"
     CDS             135..479
                     /locus_tag="N7B39_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HGU2423748.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALGDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(483..2072)
                     /gene="yejF"
                     /locus_tag="N7B39_003851"
     CDS             complement(483..2072)
                     /gene="yejF"
                     /locus_tag="N7B39_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HGU2423749.1"
                     /translation="MTQTLLAIEDLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNYAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIDVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(2074..3099)
                     /locus_tag="N7B39_003852"
     CDS             complement(2074..3099)
                     /locus_tag="N7B39_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU2423750.1"
                     /translation="MSRLNPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            complement(3099..4193)
                     /locus_tag="N7B39_003853"
     CDS             complement(3099..4193)
                     /locus_tag="N7B39_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HGU2423751.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            complement(4194..6008)
                     /locus_tag="N7B39_003854"
     CDS             complement(4194..6008)
                     /locus_tag="N7B39_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HGU2423752.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYSGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDHSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            complement(6090..7646)
                     /locus_tag="N7B39_003855"
     CDS             complement(6090..7646)
                     /locus_tag="N7B39_003855"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HGU2423753.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLITDVQKYLDTYFADLKSTTDRLQPLTLDTCKQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKANIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            complement(7827..8393)
                     /gene="mepS"
                     /locus_tag="N7B39_003856"
     CDS             complement(7827..8393)
                     /gene="mepS"
                     /locus_tag="N7B39_003856"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HGU2423754.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
BASE COUNT         2278 a         2164 c         2172 g         2049 t
ORIGIN      
        1 tacttaaatc agagccatag aggccacgca ggcgaggcat caatctttac gatctgtata
       61 aagacggatt gttgatgatg tgttaaaatt gatgtaaaca aattgtgaag taaatgtgct
      121 tccggggaaa ataagtgact tcattacaac tctcaatcgt ccatcgactg ccgcagaact
      181 atcgctggtc tgctggtttc gcaggttcga aggttgaacc gattccgcaa aatggaccgt
      241 gcggtgacaa cagcctggtg gcgcttaaat tgcttagccc ggatggtgat aatgcatggt
      301 cggtgatgta taaactaagc caggcgttag gcgacatcga agttccatgt tcggtgctgg
      361 agtgtgaagg tgagccgtgc ctgtttgtaa atcgccagga cgagtttgct gcaacatgcc
      421 gattgaaaaa ttttggtgtg gcaattgccg aacccttttc aaactacaat cctttttaag
      481 cgtcagctca acgccagtag ctgacgcgta tactcctgct gcggtgtggc aaacacgcgc
      541 gcgcacggtc cttgttccac tacttcccct tgtcgcagta cgataacctg atgacataac
      601 gcgcggacaa cgtgcaaatc gtggctgata aacaaatagg ccagttgatg cttttgttgc
      661 aatgatttca atagcgtcaa tatttgcgcc tgtaccgttt tgtcgagtga tgatgtcggt
      721 tcatcaagta tgatcagcga gggcttaaga attaatgccc tggcaatcgc aatacgttgt
      781 cgctgaccac cagagaactc cgccggataa cggtggcgtg tttcaggatc taatcccact
      841 tcatgcatca cgtctatcac ttgttgttcg cgttgtgcgg cagaaagcgt cggctggtga
      901 acccgtaagc cttcctcaat aatctgcaaa acgttgagtc gtgggttgag cgaggagttt
      961 ggatcctgaa ataccacctg aatgcgatga cgaataggta acagctggcg gcgatttaaa
     1021 ttttgcagtg gctgaccgtc aaagatgatg ctgccctgag aattaatcag tcgcagcagc
     1081 gccagtcccg tcgtgctttt cccggaaccg gactcgccca ctaaacccag tgtttcaccc
     1141 gctcgtagcg taaaactgat gtttttcacc accacattat gatccacaat gcgcttcaaa
     1201 atccctttgc gaatggggaa ggcaacctga agctgttcaa catccagcaa cgttgaggca
     1261 ggttctggca acggcactgg atcgcctgac ggttcactgt tgagtagctt ttgtgtgtaa
     1321 ggatgagtgg gtgaggcaaa tagcgtagcg gcgtaatttt gctcgacaca gcgaccgttt
     1381 tgcattaccg ccacgcggtg ggccagtttt ctgacaatgc tgaggttatg agtaataaac
     1441 agcatgccca tattcaactc gccttgcagt tcgcgcaaca gctgtaaaat ctgcgcctgg
     1501 acagagacgt ccagtgcggt ggtcggttca tcggcaatta ataattccgg tcgcgttaac
     1561 agcgccatcg caatcatcac ccgctggcgt tcgccgccgg agagctgatg cggataatct
     1621 gtcagccgtt ttgccgcctg gcggatacca acgcgatcaa ggcagttaag aatttcgcca
     1681 cgagccgctt cccgacgcat cccgcggtgg agtgaaagca cttcataaag ctgtttttcc
     1741 agggtatgca atggatttaa cgacaccatc ggctcttgaa aaatcatggc gatcttatta
     1801 ccgcgtacac cgcgcaacgt ttggtcgctg gcatgaagca gcgattcgcc atgaaaacga
     1861 atatcgccgg agagatattc aaccggcggg gaagggagca ggcgtaaaat tgacagcgcg
     1921 gtaacgcttt tgcctgaacc tgactcaccc accagcgcca gcgtttcgcc agcctcaatc
     1981 tgtagtgaaa catcattgac tactgtacgt acggtttgct gatggcgaaa acccaccgac
     2041 aaatcttcaa tcgctaacag agtttgcgtc atgctacacc gccttattag gatcaaatgc
     2101 gtcgcggacg gcttcaccaa taaagatcag caaagacaat aatatcgcca ccgacaagaa
     2161 ggcggtgatc ccaagccacg gagcctgaag gttatttttc ccttgtaaca gcagttcacc
     2221 gagtgacggt gaaccgagcg gcagaccgaa gccgaggaaa tcgagtgagg tcagggtggt
     2281 gatcgaacta cataaaataa acggtaaaaa ggtgagggta gcgaccattg cattcggcaa
     2341 catatgacgc agaataatac tgcgatcgct gacgccaagc gcctgtgccg cgcggatgta
     2401 gtcgaaattg cgggtacgta aaaactccgc ccgcaccacg ccgaccagac tcatccagcc
     2461 aaataagaca gtaattgcca gcagccacca gaagttaggt tgcacaacgc tggaaagcag
     2521 aataatcaaa aacagcgttg gcatccccga ccatacttca ataaagcgct gcccccagag
     2581 atcgacctta ccgccgtaat agccttgtag cgcccccgcc agcacgccca tcacgctgga
     2641 acagagagtc agcatcaggc caaacagaac cgagatccgc gtgccataga gaatacgtgc
     2701 cagcacatcg ccgccgttgg catccgttcc cagccagttt tgccgggagg ggggagaagg
     2761 gaagggctta tcggtagcaa agttgatact ggtagcacca aagcgaatcg gtgcccacag
     2821 tacccagcca ttattttcca gccgttgttt cagccacggg tcctgataat cagcctgact
     2881 tgccagcggg ccgccaaaat cgctttcgct gtagtttttc aacaacggga aataccaact
     2941 gccgtcataa cgcaccagca acggtttatc gttggcgata agttcagaac acaaactcaa
     3001 accaaacaaa acgaggaaaa tccataacga ccagtagccg cgacggttat ggcgaaaacg
     3061 cgcccaacgg gcctgattga cggggttgag tcgcgacatt aacgtccctc aaaatctata
     3121 cgcggatcga ccagcgtata gctgatatca ctgacaatat tcagcagcag gccaatcagg
     3181 gtgaaaatat acaaggtacc aaacattaca ggataatcgc gcgagacggt cgcttcgtag
     3241 cccagtaagc caagaccatt gagtgaaaac atcacctcaa tcagcagcga gccggtaaaa
     3301 aacatgctga taaacgtcgc cggaaaaccg gcaatcacca gcagcatggc gttgcggaac
     3361 acatgtttcc agagaatatt tttttcactt acccctttcg cacgcgcggt caccacgtat
     3421 tgcttgcgta cttcatcaag gaatgagttt tttgtcagca tagtcagcgc cgcaaagcca
     3481 ccaatcaccg tcgccagcac cggcagcgtg atatgccaca gataatcggt gattttctga
     3541 taccacggca gcgaatcaaa gttagcggaa accaggccgc gtagagggaa caggtcgaaa
     3601 tagctgccgc ctgcgaagaa gacaatcagc aggatggcaa acaaaaaggc cggaatggcg
     3661 tagccgatga tgataaatgc gctactccag acgtcaaagc ggctcccatt ataaacagct
     3721 ttgcgaatgc ctaacggaat cgacaccaga tagataatca gcgtgctcca caatccgagg
     3781 gtgatggaaa ccggcagact gtctttaatc agcgtcagca ccgaggcgct gcgaaacagg
     3841 ctatcgccaa aatcaaagcg gatgtagtcc cagagcattt tgaagtaacg ttcgtggatc
     3901 ggcttatcga aaccgtagcg atgagtgatc tcggcgatca cttctggatc taatccgcgt
     3961 ccgccacggt aattactgtc gctgatatta ccgacgcccg tttgcgcatg gctggcgcga
     4021 acgccttcac cgcctgcgcc gggtaatact ccggcattac caaactcaat ggcggcgatg
     4081 gcctggtcga ccggaccgcc aggcgcaatt tgcacgatga aaaagttgat agtgataatc
     4141 gcccataacg ttgggatcac cagcaacaga cggcgaatca gataagcgcc catctactct
     4201 ccctgttgtc tggctgacgg cagtttggcc gctttattaa catcatacca ccaggtatcg
     4261 atacccagac tgtaaacagg gcgcacagcg ggttgggaga atttatccca ccaggcgaga
     4321 cggtcttccg ccatgtacca cattggcagc atgtaataat tccacgttaa tacgcgatcc
     4381 agtgctcgcc ctaacggcag taatttttct ttatttccct gcgcggcaat aatttggttg
     4441 atcagcgagt cgataaccgg gctttgcacg ccgggggcat tataagtgga attgatatat
     4501 tccgatgacc aggaaatctg taaatcggaa ctgggccacg gcatcgcccg ccataggcgc
     4561 ggcatcatgt catagtcgcg actgcgcatg cggttggtga tttgcgagtg atccaccttg
     4621 cgaatgtcca tgttgatacc cagccgttgc aggctgtgct ggaacggcaa tacccactga
     4681 ctattgctgc ttgcaggaag caataattca aagctgagcg gctgacccgt agtggcatta
     4741 acgcgttgct gacctttcag cacccagccc gcttcgttga gaagtttgtc ggcttttaac
     4801 aggttgtcac gatcgtagcc atcgcctttc gataccggcg gctggtagat ttgtgtgaag
     4861 acttctggcg gtaaatcttt tttcattggt gccagaagca ccagctccgc ggcgtcgggg
     4921 taatttctgg ccgcgtattc ggtattctga aaataactgt tcgtgcgact ccaggcattg
     4981 taaaacaacg ccttgttcat ccattcaaag tcaaaggcga gggtgattgc ttcccggacc
     5041 cggcgatcgc tgaataccgg acgttggata ttaaacgcca gccagcgcgt atcctgggct
     5101 gattcgttct tttgctcatc tttgatgatg tattttttat cgaaattttt accgctatag
     5161 cgtgtggccc agtttttggc gtcgttttcc atacgcaaat caaaggcacc tgctttaaac
     5221 gcttcaaagg cgacattatc atcgaggtaa taatcgtagc gaatggtgtc gaaattccag
     5281 cgtccacggt ttaccggtaa gtttgctgcc cagtaatctt tcacgcggga atagacaata
     5341 ttttgcccca ttttccagga cgtaattcgg tacggaccac tggcaagcgg aggcgtggcg
     5401 agcggatcgc taagtttgtg atccttccag tacttttctg gaaataccgg cagcgaaaac
     5461 agactcaaca tatcttcttt gccgggttta gctaactcaa tgcgcacggt taacggcgca
     5521 atggctttga cggtggtgcc tttgtagacc agacgaaatt gcggcacgcc ttcagtcata
     5581 aatttttgaa aagtaaactc tacatcgcgg gcagtaatgg gcgaaccatc atgaaaacgg
     5641 gcgcgaggat taatagcgac ctccacccag gaataatcgt cagcatagcg tgcgctttca
     5701 gcaatcagcg ggtaataact gcctggttcg tcatcggaag tcgtaaatag cgtgtcgtac
     5761 agctgctcgg tgcgtgcgcc cgggttgccg cgcagtgcat agcggttgaa attatcgaag
     5821 gtaccgaggg ctgacaacgt tatctgccca ccttttggcg cggcggggtt cacataatca
     5881 aaatggttga aattaaacgc gtaccggggt tcgcccagca cggcaaaagc atagctttcc
     5941 ttgatagcct gcgcctgcgc accaaaggtg aacagagcga taaacagcag cagtatgcgc
     6001 acaatcatct gcaacggatt tccttttact catcattagc cgcaatccgg catgctttca
     6061 ctatgaataa taagggacgt aagcacacct taccactgcg gcgtatacgg tttcttcagc
     6121 cagcggacaa aatcgtccag cggtaacggg cgactaatcc agtagccttg catgaaatta
     6181 acgccgcgtt cgcttagcca tcgcgcctgt tccggcgttt cgaccccctc agcaaccgtc
     6241 agcatattga ggcgtttcgc cagcgtcagc accgcgtcaa gtacgggtga agtgatcgtt
     6301 tccgtaccga tggcgttgat aaatccacgg tcaattttca gataatcgag cgtaaaacgc
     6361 tcaagataga taagcgcgct gtgcccggtg ccgaagtcat caatagcaat ttctacgccg
     6421 accgagtgca gccaggcgaa gagttgtgtg gcttcttgct ctttcagcat atcgcgctcg
     6481 gtaatttcca gcacaatctg gaaatggtgt gcgggtaggg aagtgagcag tttctggata
     6541 tttgctttaa agctttcgct gtgcagatgg tccggcgcaa tgttaatgcc aaatttgacg
     6601 cctaccggca gcactttttc taattctgcg gcatcgcggg caattaactc aaacaggtgc
     6661 tgagtcagcg gcacaatcat cttttgcgat tcggcaaagt taatgaaggc atccggggga
     6721 atttctcccg cgacaggatg ccgccagcgt agcagtactt ccaggcccgt tactcgcaaa
     6781 gcttgtgtat ccaccaccgg ttgatacgcc acgtaaaatt gttcgcgctt gatggcggtc
     6841 atgatttctc tgccggggcg catacgtacg ctcatcaggt aatagcagag caggccaacg
     6901 acagttccac tcatgccgcc cagtaaaaat gcgtaccaca catcgttcca tgtccagtca
     6961 tctgcataaa gccgaacggt cagaggaatg cccgcaattt tagtttcacg gactggcatg
     7021 tcggttaatt cgttaacgtt catcaaacgt gaagaaaagg tagatagcgc agtattgcca
     7081 ataatgaggg cgacgccatc gtaatcttcc tgccgtgagc tataaaagag agaaggcgtc
     7141 aggttgagat tcagagcggc aaagacgccg ctatttttca gcaaagggtt gcgataccag
     7201 atgacgattg cgggtttgtt cggcaccatc ggcgtgccgg gtaagatcgc catatcgaca
     7261 tttttattaa tgtcgagcgc aggaatcaat tcattgagtg gaatgtccat ctcaccggtc
     7321 gcagatgaac agaatgtttt tttatctttc accagcacaa acgttcggac attcatgcta
     7381 aacgccgcgc gggcggtcag ttcggggtta gcttgcttgc atgtatctaa ggtcagcggc
     7441 tggagccggt cagtagtgga tttcaggtcg gcaaaatagg tatcgagata cttttgtacg
     7501 tcggtaatca gtgtgtcgta tttgacttcg tgcttatgcc aggccactaa aaactgaagg
     7561 cagctcacca gtatcgcaat cattacgcct gcaacaatgc aggtaagcag gagcttacgt
     7621 ccagaattgg gagcgcgtat gaacatgatt ccgttaattt cctgaaatta cgacaacatt
     7681 taaaagataa caatgaccgg actatagcct ggatcaagac gcggaaacat cattttctct
     7741 gatgaatgaa aacaaaaaag cactgcctaa acagtgcttt cagttaactc gtcaggatag
     7801 ccaagggatt gcatccaaac ggtttattag ctgcggctga gaacccggcg tgcttcgttg
     7861 taacgcttct tccagtacgg ttcattcatg ctggaaataa taacaccact gctggtggaa
     7921 gcatggacaa actgattgtt gccgatataa ataccgacat ggcgtcccgt tgaaccggca
     7981 cggaacagaa ctaaatcacc cgtacgcaaa ttactgcggg aaacagattt acccatttcc
     8041 tgctgttcgt aagtcgaacg cggaagttct aagccaaatt gctcacggaa tgtacgctgt
     8101 acgaaaccag aacaatcgat acctttttta gtgctgccgc ccagacgata acgtacgcct
     8161 ttccagtcag catactgatc cataattcgc gattttacgt cgacattacg aaccaggttt
     8221 tcaaattcat cctgagaagc ttgcagtgat gatgtttcac tacccactgc acgtgtctca
     8281 ggatgcatat tctttgcggt gttatttgca ctacatgcag aaagcagaac cgctactgca
     8341 atcgcgggga tcccgcgcaa gatatatctc aaaatcggtt gagatttgac catgttgttt
     8401 gttttccctt gaagtcctta acgacaaata tcgttataaa aaaaatgcca agcgacacga
     8461 gactaattac cagggcatgt tgaaacaatt ccttaaggcc aaatctgtgg actaacgcac
     8521 aaaattatat gctgtgagga taatctaccg ccgaggcaaa acgagttcga gattacccga
     8581 ttggtgcggt cttggcgagc ggttttacat gattttttat aagaaaaagt gatacagaaa
     8641 gttaataagc gggattggtc aat
//
LOCUS       DBFIFI010000180         8621 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.180, whole genome shotgun sequence.
ACCESSION   DBFIFI010000180 DBFIFI010000000
VERSION     DBFIFI010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8621
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.180"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            311..4294
                     /gene="ehaA"
                     /locus_tag="N7B39_003857"
     CDS             311..4294
                     /gene="ehaA"
                     /locus_tag="N7B39_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
                     /protein_id="HGU2423755.1"
                     /translation="MNKIYRLKWNRSRNCWSVCSELGSRVKGKKSRAVLISAISLYSS
                     LVFADDVIVNQDKTIDFGKENQSIDYRITVTDNANLVINATDTSRPRLTLASGGGLDI
                     TGGKVTINGPLNFLLKGTGFLNVSNAGSELYADDLYESNSGMRHDRGYFNVSNGGKIH
                     VKGTSRLTYLQGNVSGEGSQVNSETFFMGVYGSYGGNQYLSVNNGGEVNARKQISLGY
                     YDQVSDTTLAVSEGGKISAPTISLSTNSELALGAQEGSAAKAAGIIDAEKIEFVWAKT
                     SEKKITLNHTDKDATISADIVSGSEGLGYINALNGTTYLTGDNSAFSGKVKIEQNGAL
                     GITQNIGTAEINNRGKLHLKADDSMTFANKISGNGTISIDSGTVELTGNNYAFSGYID
                     VASGAVAVISEDKNIGRAELDVDGKLQINANKDWVFDNDLEGRGIVEINMGNHEFSFD
                     EFAYTDWFQGSLAFQNTTFNLEKNAEFLQKGGITAGQGSLVTVGKGAHSISTLGFSGG
                     TVDFGALTAGAQMTEGTVNVSKTLDLRGEGVIQVSDSDVVRSVSRDIDSALSLTEVDD
                     GNSTIKLVDAQGAEVLGDAGNLQLQDKNGQILSSSAQRDIQQNGQKAAVGTYDYRLTS
                     GVNNDGLYIGYGLTQLDLHATDSDALVLSSNGKSENAADLSAKITGSGDLAFSSQKGQ
                     TVSLSNKDNDYTGVTDLRSGTLLLNNDNVLGNTHELRLAAETELDMNGHSQTVGTLNG
                     SADSLLSLNGGSLTVTNGGTSTGSLTGSGELNIQGGTLDIAGDNSNLTANVNIANSAN
                     VLVSHAQGLGSANVENNGTLALNNSAEKRAAASVNYALGGNLTNNGTLMTGMSGQQAG
                     NVLVVKGNYHGNNGQLVMNTVLNGDDSVTDKLVVEGDTSGTTAVTVNNAGGTGAKTLN
                     GIELIHVDGKSEGEFVQAGRIVAGAYDYTLARGQGANSGNWYLTSGSDSPELQPEPDP
                     MPNPEPNPNPEPNPNPTPTPGPDLNVDNDLRPEAGSYIANLAAANTMFTTRLHERLGN
                     TYYTDMVTGEQKQTTMWMRHEGGHNKWRDGSGQLKTQSNRYVLQLGGDVAQWSQNGSD
                     RWHVGVMAGYGNSDSKTISSRTGYRAKASVNGYSTGLYATWYADDESRNGAYLDSWAQ
                     YSWFDNTVKGDDLQSESYKSKGFTASLEAGYKHKLAEFNGSQGTRNEWYVQPQAQVTW
                     MGVKADKHRESNGTLVHSNGDGNVQTRLGVKTWLKSHHKMDDGKSREFQPFVEVNWLH
                     NSKDFSTSMDGVSVTQDGARNIAEIKTGVEGQLNANLNVWGNVGVQVADRGYNDTSAM
                     VGIKWQF"
     gene            4436..5524
                     /locus_tag="N7B39_003858"
     CDS             4436..5524
                     /locus_tag="N7B39_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HGU2423756.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMAGVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(5566..6497)
                     /locus_tag="N7B39_003859"
                     /pseudo
     CDS             complement(5566..6497)
                     /locus_tag="N7B39_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
     gene            complement(6589..7096)
                     /locus_tag="N7B39_003860"
                     /pseudo
     CDS             complement(6589..7096)
                     /locus_tag="N7B39_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
     gene            7354..7959
                     /locus_tag="N7B39_003861"
     CDS             7354..7959
                     /locus_tag="N7B39_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HGU2423757.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNGDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            7999..>8621
                     /locus_tag="N7B39_003862"
     CDS             7999..>8621
                     /locus_tag="N7B39_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HGU2423758.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFRLIAWQQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVIDKQNI
                     ASAVASMMGLGIGLTPSADDYLTGLALILFISGHPAGKYKEEFYLGL"
BASE COUNT         2380 a         1887 c         2235 g         2119 t
ORIGIN      
        1 atattaaatg tatttctcct gcttttgttt atattttgag ttgatttgag ttcccggata
       61 catataatgc gcaggtatta gagactgggg gggaatgtaa tgataaagtt aagagtgtta
      121 acttaaaata agtttttagt agtaacttgc tattttatta ccctctgaac tacatactca
      181 gatatgtggt tgccaatgct atgcaaaatt tttgaatttt tatatgcgat gaacagtaac
      241 cggaatggca tttaatgcgt tgctgtttat gcaatcttgg ttttatttgg atgtattgct
      301 ggagattgtg atgaataaaa tatatcggct aaagtggaac aggtcccgta actgttggag
      361 cgtctgctcg gagctgggga gcagagtaaa aggaaaaaag tcccgggctg ttttaattag
      421 cgcgataagt ttatattcat ctctggtatt cgccgatgat gtcatcgtaa accaggataa
      481 aactattgat tttggcaaag agaaccagag catcgattac cgtattacgg tgacagacaa
      541 tgccaatctg gtaatcaatg cgacagatac ttcccgtccg cgtctgactc tcgcttctgg
      601 tggtgggttg gatattaccg gaggaaaggt aacaatcaat ggcccactta actttttgct
      661 gaaaggtacg gggttcctga atgtctccaa tgctggcagc gagttatatg ctgatgattt
      721 gtatgaatca aactcaggca tgagacacga tcgcggctat tttaatgtct ccaacggcgg
      781 caaaatccat gttaagggca ccagccgtct gacctatttg cagggaaatg tcagtggtga
      841 aggtagccag gtaaattccg aaaccttctt tatgggcgtt tacggcagtt acggtggtaa
      901 tcagtacctg tcagttaata acggcggtga agttaatgcc aggaagcaaa ttagcctggg
      961 ctattatgat caagtctccg atacaacact tgctgtttcg gaaggtggta aaatttctgc
     1021 gcctactatt agtttaagca ccaactctga gttagcgtta ggggcacagg aaggaagcgc
     1081 agcgaaggca gcagggatta ttgatgccga aaaaattgag tttgtgtggg caaagacatc
     1141 cgagaagaaa atcaccttaa accacacgga taaagacgcg actatttccg cggatattgt
     1201 cagtggcagc gagggcctgg gctatatcaa tgcgctcaat ggcacgactt acttaaccgg
     1261 tgataactct gcctttagtg gtaaagtcaa aattgagcaa aatggcgctt tagggatcac
     1321 ccaaaatata ggtacagcag agatcaacaa ccgcgggaaa ttacacctga aggctgacga
     1381 tagcatgacc tttgccaata agatctctgg caacggtaca ataagtatcg acagtgggac
     1441 ggtggagttg accggcaata actatgcatt cagcggatat attgatgttg cttctggtgc
     1501 tgtcgctgtt atttctgaag acaagaatat cggtcgtgca gagctggatg tcgatggcaa
     1561 attgcaaatt aatgccaaca aagattgggt atttgataac gatcttgaag gtagaggcat
     1621 tgttgaaata aacatgggga atcacgaatt ctccttcgat gagtttgctt atacagactg
     1681 gttccagggt tcactggcgt tccagaacac gacatttaat ctggaaaaga atgctgagtt
     1741 tctgcagaaa ggcgggatca ctgcgggtca gggaagcctg gtaacagtgg gtaagggcgc
     1801 tcactccatt agcactttgg gattctccgg cggaaccgtt gattttggtg ccctgacagc
     1861 aggtgcacag atgacagaag ggacggtcaa cgttagtaaa acgctggatt tgcgcggcga
     1921 gggtgtgatt caggtttctg acagtgacgt tgtccgctca gtatctcgtg atattgactc
     1981 tgcgttatcg ctcactgaag tcgatgatgg taacagcacc attaagttgg ttgatgcgca
     2041 aggtgcggaa gttctgggcg atgcgggcaa tctgcaattg caggataaaa atgggcaaat
     2101 cctctccagc agcgcccaac gtgatattca gcagaatggg caaaaagcgg ccgtcggcac
     2161 ttacgactat cgtctgacga gtggggtaaa caatgacggt ctgtatattg gttacggcct
     2221 gacccagctt gatttacacg ctaccgacag cgatgctctg gtgctgagct ctaacggtaa
     2281 aagcgagaat gccgccgatc tcagcgcaaa gattaccggc agtggtgacc tggcattcag
     2341 cagccagaag ggtcagaccg tatcgctttc taacaaagac aacgactata ccggtgttac
     2401 cgatctgcgc agtgggacgc ttttgttgaa taacgataac gtgttgggta atacccatga
     2461 actgcgtctg gcggcagaga ctgaactgga catgaatggt cacagccaga ccgtgggcac
     2521 gctcaatggc agcgccgatt cactgctgag cttaaatggc ggcagtctga cggttaccaa
     2581 cgggggcact tcaaccggtt cgttaacggg gagcggagag ctgaatattc agggcggcac
     2641 gctggacatc gcgggcgata acagcaacct gacggcgaat gtgaacattg ctaattcggc
     2701 taatgtcctg gtaagtcatg cgcagggatt gggtagcgca aacgttgaga acaacggtac
     2761 cctggcgttg aataatagcg ctgaaaaaag agcggctgcg tctgtgaatt acgccctggg
     2821 cggcaatctg accaacaacg gtacgctgat gaccggaatg tcaggacagc aagctggcaa
     2881 tgtgttagtg gtgaagggga actaccacgg taataacggt caactagtaa tgaatacggt
     2941 actgaatggc gatgactcag taaccgataa attggttgtc gagggcgata ctagcggcac
     3001 gactgccgtt acggtgaata acgctggcgg tacaggtgcg aaaaccctta acggtatcga
     3061 acttatccat gtagacggta agtctgaggg cgaatttgtt caggctgggc gtatcgttgc
     3121 gggggcgtat gactacactc tcgcgcgtgg acaaggggca aatagtggta actggtatct
     3181 gaccagcggc agtgattctc ctgaactgca gccggagcca gacccgatgc cgaatccaga
     3241 gccaaacccg aatccagagc cgaaccctaa cccgacacct acgccgggtc cggatctgaa
     3301 tgtggataat gacctgcgac cggaggcggg tagctacatt gcgaaccttg cagcagcgaa
     3361 taccatgttc accacgcgtc tgcatgagcg tctgggtaat acgtactata ccgacatggt
     3421 gacgggtgag cagaaacaaa ccactatgtg gatgcgccat gaaggtggtc ataataaatg
     3481 gcgtgatggc agcggccagc tgaaaaccca aagcaatcgc tatgttctgc aactgggagg
     3541 cgatgtcgcg cagtggagcc aaaacggcag cgaccgctgg catgttgggg tcatggcggg
     3601 atatggcaac agcgacagca aaaccatttc ctcgcgaacc ggttatcgtg caaaagcgag
     3661 tgtgaacgga tatagcacag gcctctatgc cacctggtat gccgatgacg agtcgcgtaa
     3721 tggcgcgtat ctcgacagtt gggcgcagta cagctggttt gataacacag tgaaagggga
     3781 tgacttacaa agtgaatcct ataaatcaaa aggatttacc gcttcactgg aagctggata
     3841 caaacacaaa ttagctgaat ttaatggcag ccagggaacg cgtaatgaat ggtatgttca
     3901 gccgcaagca caggttacct ggatgggagt caaagccgat aagcaccgcg aaagcaacgg
     3961 aaccctcgtt catagcaacg gtgatggcaa tgttcaaacc cgacttggcg taaaaacctg
     4021 gctgaagagc caccataaaa tggatgacgg taaatcccgc gagttccagc cgtttgtaga
     4081 agtgaactgg ctacataaca gtaaggattt cagcaccagt atggatggcg tgtctgtcac
     4141 tcaggatgga gcccgaaata ttgctgagat aaaaaccggg gtggaaggac agctaaatgc
     4201 caacctgaat gtctggggga atgtgggcgt tcaggttgcc gataggggat ataatgacac
     4261 ctctgcaatg gttggcatta agtggcaatt ctgatctgaa taatgagtaa aaggagagag
     4321 agccgaacgg gagataattc ggctctctgt gtcattttct tgcagccagc tttatccgtc
     4381 agagtgaaga ctcgcagatt tagctggcgc acaagattta atcaaggtgt gttcaatggc
     4441 ttcgcatgat ttgagtgttt ttttggaaga gttcggggca acggtgaatt tgacgttgcc
     4501 tggtatcgtg tccgaaaaag aacggctttt actcaagctg ctaatggagg gaatgtcggt
     4561 tacagaaatc tcacagtaca gaaatcgcag tgcaaagaca atttcacatc aaaagaagca
     4621 gctgtatgaa aaactaggca tccagagtga tattaccttc tggcgggata tcttttttca
     4681 atatcatccg caagtcatta gtggcacggg aaataaaaat aatttttata tccctgataa
     4741 ccgttgtcac catatcgtca cgcctgaagc catcagtctg gcgttggaaa accatgaatt
     4801 taaaccgtgg atccaaccgg ttttctgcgc gcagactggg gtactgacgg gctgtgaagt
     4861 gcttgtccgc tgggaacatc cacaaacggg aatcatcccg ccggatcagt ttattcctct
     4921 ggcggagtca tccggtctta ttgtcataat gacccgccaa ctgatgaaac agactgcgga
     4981 tattctgatg ccggtaaaac atttgctgcc ggataatttc catattggta tcaacgtctc
     5041 ggcgggttgt tttttggccg cgggatttga aaaagaatgc ctgaacctgg tcaagaaatt
     5101 aggaaacgat aaaatcaaac tggttctcga gctaacggaa cgtaacccta ttccggtaac
     5161 gccagaagcc agagcgatat ttgacagcct tcatcagcat aacattacct ttgcgctgga
     5221 tgactttggt acgggttatg cgacctatcg ttatctgcag gcgtttccgg tcgattttat
     5281 taagatcgat aagtcatttg tgcaaatggc gggtgtcgac gaaatatccg gtcatattgt
     5341 ggacaatatt gtcgaactgg cgcgtaagcc tggtctgagt atcgtggcgg aaggggtaga
     5401 aacccaggag caggcggatt taatgatcgg taaaggcgtt cactttttgc agggctattt
     5461 gtactctccg ccagtaccgg gtaataaatt tatctctgaa tgggtaatga aagcaggtgg
     5521 ttgatgtaaa ccgctattca cagcgcatcg ggaggttggc agcgattaat tctcccgatg
     5581 cagtaaatcc tgatagatcc ccgttaatat gccatttggc gcaaattgtt ttttaatcca
     5641 ctgcgtcacc tgacccgtag cagaatgctg agtcgccagt aacatgcgtg aatcctggcg
     5701 cgggttatgg atttgtcggg taaccagcag ggattgcgcc atcgcctcac ggaccatgta
     5761 atctggcaaa aaaacaatcc cttcacctaa aatctggcac tgacatttgg tgttgaaatc
     5821 tggcaccaga attgactcct gcccgtgcag caaccagccc acttttttat taatcgtatg
     5881 cgcggtgtcc tccaccataa tattagggta gagacgcagt tggctttcgg cgatggttcc
     5941 ggcacaaagg ctaacgggtg atccggggcg atagcaaaag cccagcgaat cgcgccaatt
     6001 tcagtgtaat caataccgcc gccgtccagc agcgtgtcgg gtgcgccgat agcgatattt
     6061 gcctgattat taataatcgc atcccagacg ccgttataca cttcggtggt gacggtaatc
     6121 tggcaggtag gaaactgctt tttcagcacc tgtaataagc gtgcggtgtg tttgggcgta
     6181 taaagcaact gattaataca aatgcgcaca cgcgcttcta tgccctgcga aatagtatcg
     6241 atgctgcgtt tgatggcgtg aaaatcattc agcaggtcgg tagctttgcg gaaaaaataa
     6301 cgcccggact ccgttaactc aatgctgcgg gtactgcgag tgaacagcac cacatccagc
     6361 cctgtttcca ttcgcttgat ggtgtagcta atggcggaag tggttaaacc caactcttct
     6421 gcggctttac tgaaactgcc aaaacgcgca gcggtagtaa atgccaacag attctcttcg
     6481 gtaaaaattg agttcattaa accattcccg ccattatcta atatgaattt ctccaagcat
     6541 aactgtagag gtatcaatta gagatgggta atcatttttg aataactttt aatacccgtc
     6601 gcgtttagtc gtcgcttcgc ttaatttttg tccgccatat tctgaaatta tccccacgca
     6661 gttacccgcc agaaaaccca gtaacgtcac cgtccagttc atcccactgg caaaaaacag
     6721 cgtcatgccg agaaagccac cgggaataaa tgacagtaac cagaaacgcc cttgccagac
     6781 caccaccgcc gagaagggta cggtagtaat tacgctcgcc cagaacgcgg acagtccact
     6841 tacagaggcc agccaaccgg cagccagcgc ggcgaaaaac gcccacacca tcccggaata
     6901 attaaccgac agacttctga taaaccccga tttaccgccg cctgcggcat aaaaactgca
     6961 aaaggcgacg aagccaatgg ttcctaataa ttcctaataa ttcccatccc tgagagagcc
     7021 cgacatgtga agacactaac tgccaaagcc ctgcacaaat accgacggtg acacccgttg
     7081 ccgtgagtcc gttcatcttg ttcccctctg gttagacatg gacgaaagct actcttacat
     7141 gtgcagacta tagagaaata aaacctgatt gagtgaaagg gtatcgggtc aaagaaacaa
     7201 atattgcact accgcacact gcgaaaagat tgttgaacat cattcaacaa aaaggcgaaa
     7261 tacggcgaat taaatttaag cgggttcttt tatcagatta tcgttgctat tctcaattca
     7321 tcgaaaacag ccacaaaaac caggggaatg tgaatgagta taaaaaatct acctgccgac
     7381 tatttattgg ctgcacaaca gggtgatatc gataaagtaa aaacctgcct tgcgctgggt
     7441 gtcgatatta atacctgcga tcgtcagggg aaaacggcaa ttacgctggc aagtttatat
     7501 cagcaatatg cttgcgttca ggcattaatt gatgccggag cggatattaa taagcaagat
     7561 catacctgtt taaatccttt tttaattagc tgtctgaacg gtgatttaac gctgctacga
     7621 attattttac cggctaaacc agaccttaat tgcgtcaccc gttttggcgg tgtcggcctg
     7681 acgcctgcct gtgaaaaagg ccatttaagt attgtaaaag agcttttggc gcatacggaa
     7741 attaacgtta accagaccaa ccatgttggc tggacgccac tactggaagc gattgtgctt
     7801 aacgatggcg ggattaaaca gcaggcgatt gtgcagttat tgctggaaca cggtgccagc
     7861 ccgcatctga ccgataaata tggcaaaacg ccactggaac tggcgcggga acggggcttt
     7921 gaagagattg cgcagttact gattgccgca ggtgcataaa ccgggaggct tgctatcaac
     7981 acaccagaaa aacggtgtgt gtgggcgcta actgcggatg cggattttct ggcgcagcga
     8041 gggcaaggac aggttgaaca ggtctttgcc agagcggtaa atatcgcact cccggctcgc
     8101 cagcagttgc tgacgctgct ttgtgaagag tacgacaatg cgccaaacag ttgtcggttg
     8161 gcactcactc actttgatga tctgttccgg catggtgata aggttcagtt tgacgatcaa
     8221 ggtattacgg ttggtcaaca tcttcatata gagatgagtc gttgtcggcg ttggctgtcc
     8281 ccaaccttgc aaatgaccgc tgtgaatttt cgccttatcg cctggcaaca gtggcacgac
     8341 atcattcatc agcacctggg ggaaaatgaa accctgttta attatcgcgg cgataatccg
     8401 ttttatcagg cgttaaataa agaattacat attaaacgac gggcagttat acaggcagta
     8461 atcgataaac aaaatatcgc ctcagcggtc gccagtatga tgggcttagg gattggcctt
     8521 acgccatcag ccgatgatta tttaacaggt ctggcactta ttttatttat ttccgggcat
     8581 ccggcgggaa aatacaaaga ggaattttat ctcggcttgc a
//
LOCUS       DBFIFI010000181         8604 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.181, whole genome shotgun sequence.
ACCESSION   DBFIFI010000181 DBFIFI010000000
VERSION     DBFIFI010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8604
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.181"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..304
                     /locus_tag="N7B39_003863"
     CDS             <1..304
                     /locus_tag="N7B39_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HGU2423759.1"
                     /translation="EDKARVLVDCYNNVYRLSLSPSIKRSAAIIKDAYIAITPDTSIL
                     HMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNISLDEFAKALKSVLARI"
     gene            complement(566..8548)
                     /locus_tag="N7B39_003864"
     CDS             complement(566..8548)
                     /locus_tag="N7B39_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HGU2423760.1"
                     /translation="MATKKRSGEEINDRQILCGMGIKLRRLTAGICLITQLVFPMAAA
                     AQGVVNAATQQPVPAQIAIANANTVPYTLGALESAQIVAERFGISVAELRKLNQFRTF
                     ARGFDNVRQGDELDVPAQVSENNLTPPPGNSSGNLEQQIASTSQQIGSLLAEDMNSEQ
                     AANMARGWASSQASGAMTDWLSRFGTARITLGVDEDFSLKNSQFDFLHPWYETPDNLF
                     FSQHTLHRTDERTQINNGLGWRHFTPTWMSGINFFFDHDLSRYHSRAGIGAEYWRDYL
                     KLSSNGYLRLTNWRSAPELDNDYEARPANGWDVRAEGWLPAWPHLGGKLVYEQYYGDE
                     VALFDKDDRQSNPHAITAGLNYTPFPLMTFSAEQRQGKQGENDTRFAVDFTWQPGSAM
                     QKQLDPNEVDARRSLAGSRFDLVDRNNNIVLEYRKKELVRLTLTDPVTGKSGEVKSLV
                     SSLQTKYALKGYNVEATALEAAGGKVVTTGKDILVTLPAYRFTSTPETDNTWPIEVTA
                     EDVKGNFSNREQSMVVVQAPTLSQKDSSVSLSSQTLSADSHSTATLTFIAHDAAGNPV
                     IGLVLSTRHEGVQDITLSDWKDNGDGSYTQILTTGAMSGTLTLMPQLNGVDAAKAPAV
                     VNIISVSSSRTHSSIKIDKDRYLSGNPIEVTVELRDENDKPVKEQKQQLNTAVSIDNV
                     KPGVTTDWKETADGVYKATYTAYTKGSGLTAKLLMQNWNEDLHTAGFIIDANPQSAKI
                     ATLSASNNGVLANENAANTVSVNVADEGSNPINDHTVTFAVLSGSATSFNNQNTAKTD
                     VNGLATFDLKSSKQEDNTVEVTLENGVKQTLIVSFVGDSSTAQVDLQKSKNEVVADGN
                     DSATMTATVRDAKGNLLNDVKVTFNVNSAAAKLSQTEVNSHDGIATATLTSLKNGDYT
                     VTASVSSGSQANQQVIFIGDQSTAALTLSVPSGDITVTNTAPLHMTATLQDKNGNPLK
                     DKEITFSVPNDVASRFSISNSGKGMTDSNGTAIASLTGTLAGTHMITARLANSNVSDT
                     QPMTFVADKDRAVVVLQTSKAEIIGNGVDETTLTATVKDPFDNVVKNLSVVFRTSPAD
                     TQLSLNARNTNENGIAEVTLKGTVLGVHTAEAILLNGNRDTKIVNIAPDASNAQVTLN
                     IPAQQVVTNNSDSVQLTATVKDPSNHPVAGITVNFTMPQDVAANFTLENNGIAITQAN
                     GEAHVTLKGKKAGTHTVTATLGNNNASDAQPVTFVADKDSAVVVLQTSKAEIIGNGVD
                     ETTLTATVKDPFDNAVKDLQVTFSTNPADTQLSQSKSNTNDSGVAEVTFKGTVLGVHT
                     AEATLPNGNNDTKIVNIAPDASNAQVTLNIPAQQVVTNNSDSVQLTATVKDPSNHPVA
                     GITVNFTMPQDVAANFTLENNGIAITQANGEAHVTLKGKKAGTHTVTATLSNNNTSDS
                     QPVTFVADKTSALVVLQISKNEITGNGVDSATLTATVKDQFDNEVNNLPVTFSTASSG
                     LTLTPGESNTNESGIAQATLAGVAFGEQTVTASLANNGASDNKTVHFIGDTAAAKIIE
                     LTPVPDSIIAGTPQNSSGSVITATVVDNNGFPVKGVTVNFTSNAATAEMTNGGQAVTN
                     EQGKATVTYTNTRSSIESGARPDTVEASLENGSSTLSTSINVNADASTAHLTLLQALF
                     DTVSAGDTTNLYIEVKDNYGNGVPQQEVTLSVSPSEGVTPSNNAIYTTNHDGNFYASF
                     TATKAGVYQVTATLENGDSMQQTVTYVPNVANAEISLAASKDPVIANNNDLTTLTATV
                     ADTEGNAIANSEVTFTLPEDVRANFTLGDGGKVVTDTEGKAKVTLKGTKAGAHTVTAS
                     MAGGKSEQLVVNFIADTLTAQVNLNVTEDNFIANNVGMTRLQATVTDGNGNPLANEAV
                     TFTLPADVSASFTLGQGGSAITDINGKAEVTLSGTKSGTYPVTVSVNNYGVSDTKQVT
                     LIADAGTAKLASLTSVYSFVVSTTEGATMTASVTDANGNPVEGIKVNFRGTSVTLSST
                     SVETDDRGFAEILVTSTEVGLKTVSASLADKPTEVISRLLNAKADINSATITSLEIPE
                     GQVMVAQDVAVKAHVNDQFGNPILNESVTFSAEPPEHMTISQNIVSTDTHGIAEVTMT
                     PERNGSYMVKASLANGSSYEKDLVVIDQKLTLSASSPLIGVNSPTGATLTATLTSANG
                     TPVEGQVINFSVTPEGATLSGGKVRTNSSGQAPVVLTSNKVGTYTVTASFHNGVTIQT
                     QTIVKVTGNSSTAHVASFIADPSTIAATNSDLSTLKATVEDGSGNLIEGLTVYFALKS
                     GSATLTSLTAVTDQNGIATTSVRGAITGSVTVSAVTTAGGMQTVDITLVAGPADASQS
                     VLKNNRSSLKGDFTDSAELHLVLHDISGNPIKVSEGLEFVQSGTNAPYVQVSAIDYSK
                     NFSGEYKATVTGGGEGIATLIPVLNGVHQAGLSTTIQFTRAEDKIMSGTVLVNGANLP
                     TTTFPSQGFTGAYYQLNNDNFAPGKTAADYEFSSSASWVDVDATGKVTFKNVGSKWER
                     ITATPKTGGPSYIYEIRVKSWWVNAGDAFMIYSLAENFCSSNGYTLPLGDHLNHSRSR
                     GIGSLYSEWGDMGHYTTEAGFHSNMYWSSSPANSNEQYVVSLATGDQSVFEKLGFAYA
                     TCYKNL"
BASE COUNT         1915 a         2138 c         2115 g         2436 t
ORIGIN      
        1 ggaagataaa gccagggtat tagtggattg ttataataat gtctaccgtt tatccttatc
       61 accctcgatt aaacgttcag cagcaatcat aaaagatgct tatatcgcaa taactcctga
      121 cacctcaata ttacatatgg caagtgccta taataccccc gttgttgcaa tttatgctga
      181 ttacaaaacg cgatggcccg caatggccga tgtttcggag tcagtcgtcg ttgggcaaaa
      241 aattgacaat ataagtctgg atgaattcgc aaaggcatta aaaagtgttt tggcgagaat
      301 atgagacagt acttatgcat agtaatgccg atctgttatg aacggtggtg tcatcaatac
      361 tctgaggctg gtcaatcatc acagaaaacc agcctttgga taaaagctgt actcaccgga
      421 tacgatcata gtcacatcga acgcgattct tgcggtatcg catttttatt tattcatttt
      481 ctgtgagaga agggcaagct ctatagaggg aatgtaaaaa agagtgcccc gaatggagca
      541 ctctttaaga aattataacg gaaaatcaga ggtttttata acatgtcgca taagcaaacc
      601 caagcttttc aaatacgctt tgatcacctg ttgccaggga aactacgtat tgttcgtttg
      661 agtttgcggg actcgatgac caatacatat ttgaatgaaa accagcttca gtcgtgtaat
      721 gccccatatc tccccattca ctgtacagtg acccgatgcc tcgggaacga ctatggttta
      781 aatggtctcc aaggggaagt gtgtagccat tgctactgca aaaattttca gcaaggctgt
      841 atatcatgaa agcatcgccg gcgttcaccc accaactctt cactcggatt tcgtatatat
      901 agctagggcc gcctgttttt ggcgtcgccg taatcctctc ccatttgctg ccgacatttt
      961 taaatgtcac tttaccggta gcatcaacat caacccagga ggcagagctt gaaaactcat
     1021 aatcagccgc cgtttttcct ggggcaaagt tgtcattatt caactgataa tacgccccag
     1081 tgaacccctg cgaagggaat gtagttgtcg gtaggttagc accattgact aacactgtac
     1141 cgctcattat tttgtcttct gcgcgagtga attgtattgt ggtactcaga cccgcttgat
     1201 gaacaccatt caatacaggg atcagcgttg cgataccctc tccgccgcct gtaacagtgg
     1261 ctttgtactc gcctgagaaa tttttactgt agtcaattgc actaacttgc acatagggcg
     1321 cgttggtacc tgactgcaca aattccagcc cttcagaaac tttgatcgga ttgcctgata
     1381 tatcgtgcag aacaagatgt agctcagcac tatcggtaaa gtctcctttc aatgatgacc
     1441 gattgttctt aaggacggac tgcgaggcgt ctgccgggcc tgccaccagc gttatatcta
     1501 ctgtttgcat tccaccagcg gtcgtgactg cgcttaccgt gacgctcccc gttatcgctc
     1561 ctctcacgct tgttgtcgcg attccgtttt gatctgtcac cgctgttaat gacgttaatg
     1621 tggcagagcc gctttttaag gcgaagtaca cagtgagacc ttcgatcagg ttaccactgc
     1681 catcctcaac cgttgccttt aaggtactta aatcactgtt ggtggcggct atagtcgatg
     1741 gatcagcgat aaagctagca acatgggcgg tgcttgagtt gccagtgact ttcacgattg
     1801 tctgtgtctg tattgttacg ccgttatgga acgatgcagt caccgtatat gtaccgactt
     1861 tattgctggt cagaacgact ggagcctgac ctgaagagtt ggttctcact tttccgccac
     1921 ttaacgtcgc accttctggc gttacgctaa agttgatgac ctgaccctcc actggagtgc
     1981 catttgcaga agttagcgtt gccgtcagag ttgcacctgt tggggaattg acaccgataa
     2041 gcgggctgga cgccgagagt gtcagttttt gatcgattac caccagatcc ttctcataag
     2101 aggatccatt cgccagggat gctttcacca tatacgaacc gtttctttcg ggcgtcatag
     2161 tgacctcggc tataccatgc gtatcagtag agacaatatt ttggctgatg gtcatgtgct
     2221 ctggtggttc tgcactgaat gttacagatt cattaagaat cggattgcca aactggtcgt
     2281 tgacgtgagc tttaactgct acgtcttgtg cgaccatgac ctgaccttca ggtatctcca
     2341 gactggtaat cgttgcagaa ttaatatctg cttttgcatt cagtaatcgc gagatgactt
     2401 cagtaggttt atctgccaga gaggctgaaa ctgttttcag tccgacctcg gtgcttgtca
     2461 caagaatttc agcgaaaccc cgatcatccg tttcaacgct ggtgctgctt agcgtgacgg
     2521 aagttccgcg gaaattaact tttatacctt ctaccgggtt gccgttagcg tcagtgacgc
     2581 ttgcagtcat ggtcgcgccc tcggtcgtgc tgacgacgaa tgagtataca gaggttaagg
     2641 aggctagttt tgcggtacca gcatcggcaa tcaaagtcac ctgtttcgta tcactgacac
     2701 cataattgtt cacgctaact gtcacggggt aggtgccgga ttttgtaccg ctcagtgtaa
     2761 cttcagcctt gccgttgatg tcagtaatgg cggaaccgcc ttgtccgaga gtaaagcttg
     2821 cgctcacatc tgccggtagc gtgaatgtca ccgcctcatt ggctaacggg ttgccgtttc
     2881 catcagtcac tgttgcctgc agcctggtca tcccgacgtt attagcgata aaattgtcct
     2941 cggtaacgtt aagattaacc tgcgcagtga gtgtatccgc aataaagttc accaccaact
     3001 gctcactctt accgccagcc atcgatgctg taacagtatg agcgcctgct tttgtacctt
     3061 tcagcgtgac tttcgctttg ccttcagtat cagtaaccac tttaccgcca tcgcccagcg
     3121 tgaagttcgc cctcacatct tccggcagag taaatgttac ctcactgttg gctatcgcat
     3181 tgccctctgt atcagcgact gttgctgtta gtgtcgtgag atcgttattg ttggcaatta
     3241 ccggatcctt cgaggctgcc agcgagattt cagcattcgc tacgttcggc acataggtca
     3301 ctgtttgttg catcgaatcg ccattttcga gggttgccgt cacttggtat accccggctt
     3361 ttgtagcggt aaagcttgcg taaaaattgc cgtcgtgatt ggtcgtatat atagcgttat
     3421 tactgggggt cacaccttca cttggtgaaa cgctgagggt tacctcctgc tggggtactc
     3481 cgttgccgta attatccttc acctcaatat acagattggt agtgtcgcct gcggagactg
     3541 tatcaaaaag tgcctgtagc aaggtgagat gtgccgtaga cgcatcagcg ttgacattaa
     3601 ttgatgtgct aagcgtggag ctaccatttt ccagactggc ctcaacggta tccggtctcg
     3661 ctcctgattc tatcgaggag cgggtattgg tataagtgac ggtagcctta ccctgttcgt
     3721 tcgtcacggc ttgaccgcca ttcgtcattt cggctgtcgc tgcgttgctg gtgaagttca
     3781 cagtcacacc tttcaccgga aagccattat tatcaacgac tgtggcggtg atgacgctgc
     3841 cggagctgtt ctgcggggta cctgcgatta tgctgtctgg gacaggcgtc aactcgataa
     3901 tttttgccgc cgctgtgtcg ccaataaaat gcacagtttt gttgtcgctg gcaccattat
     3961 tagccagtga tgcagtgacc gtctgctcac caaaggcaac gcctgcgaga gtggcctgcg
     4021 cgatgccaga ctcattggta ttactttccc ctggggtcag ggtgaggcct gaagaagctg
     4081 tgctgaatgt taccggaaga ttgttcacct cattgtcgaa ctgatctttg accgttgcag
     4141 ttagcgttgc gctatcgacg ccattacctg tgatctcatt ttttgatatc tgaagaacaa
     4201 ccagagccga ggttttgtcc gccacaaacg ttaccggctg tgaatcactg gtattgttat
     4261 tactcagcgt tgcggtaacc gtatgcgtac ccgctttttt acctttgagc gtgacatgcg
     4321 cttccccatt ggcctgggtg atggcaatac cgttattttc gagggtaaag tttgccgcca
     4381 cgtcctgtgg catggtgaag ttcaccgtta ttcccgccac cggatgattc gacggatctt
     4441 tcaccgtcgc cgtcagctgc acgctgtcgc tgttattcgt caccacctgt tgagcaggga
     4501 tgttcagcgt aacctgcgcg ttgctggcat cgggcgcaat attgactatc ttcgtatcgt
     4561 tgttgccgtt aggcagtgtg gcttcggctg tatgaacacc caaaaccgtg cccttaaagg
     4621 taacttcggc cacaccactg tcgttagtat tgcttttgct ctgactaagt tgagtatctg
     4681 cggggttggt actgaaggtg acctgtagat cttttactgc gttatcaaaa ggatctttca
     4741 ctgttgccgt cagagtcgtc tcatccacgc cattcccaat gatttccgct ttcgatgttt
     4801 gcagaacgac aaccgcgctg tccttatccg ccacgaaggt gactggttgc gcatcgctgg
     4861 cattattgtt acccagcgtg gcggtcacag tatgcgtgcc cgcttttttg cctttgaggg
     4921 tgacatgcgc ttcgccattg gcctgagtga tggcaatacc gttattttca agggtaaagt
     4981 ttgccgccac gtcctgtggc atggtgaagt tcaccgttat tcccgccacc ggatgattcg
     5041 acgggtcttt caccgtcgcc gtcagctgca cgctgtcact gttattcgtc accacctgtt
     5101 gtgcagggat gttcagggtg acctgcgcgt tgctggcatc gggcgcaata ttgacgattt
     5161 tcgtatctct gttgccgtta agcagtatgg cttcggctgt atgaacaccc aaaaccgtgc
     5221 ccttaagggt aacttcggca ataccgttct cattagtatt acgcgcgttc agactcagtt
     5281 gcgtgtctgc gggggaggtg cggaagacta ctgaaagatt tttaaccacg ttatcaaaag
     5341 gatctttaac tgttgctgtc agagtcgtct catccacgcc attcccaatg atttccgctt
     5401 tcgatgtttg cagaacgaca accgctctgt ctttatccgc cacaaacgtc attggctgtg
     5461 tatcgctgac attgctgtta gccagacgag ccgtgatcat atgcgtgccc gctaacgtgc
     5521 cggtcaggga ggcgattgca gtcccgttgc tatccgtcat gccttttccg ctgttgctaa
     5581 tcgagaaccg acttgcgacg tcgtttggca cagagaaggt gatttcttta tcttttagtg
     5641 gattgccatt tttatcctgc aaggttgcag tcatatgtag cggagctgtg ttggtgacgg
     5701 tgatatcacc tgaaggcaca ctgagggtca gggcagcagt actttgatca ccgataaaaa
     5761 tcacctgttg attagcctga gaaccagagc tcacagaggc cgtaaccgta taatcaccat
     5821 ttttcaaact ggtcagcgta gctgtggcga tcccgtcgtg gctattcact tcggtttggc
     5881 tcagtttcgc tgctgctgaa ttgacattga aggtgacctt gacgtcattg agcaggttgc
     5941 cttttgcatc ccgaactgtc gcggtcattg tggcactgtc attgccgtca gcgaccactt
     6001 catttttcga cttctgcaga tcaacctgcg cggtactcga gtcgccgaca aaactgacga
     6061 ttaacgtttg tttcacgcca ttttcaaggg tgacttcaac cgtgttgtct tcctgcttac
     6121 tacttttcag atcaaaagtc gccagaccat taacatccgt ttttgcggtg ttttgattgt
     6181 taaaggaagt tgccgatccg cttaataccg caaacgtgac ggtatgatca ttgattgggt
     6241 tgcttccttc atcagcgaca ttgaccgaga cggtgtttgc tgcattctca ttggcgagca
     6301 caccattatt gctggcagat aatgtcgcaa ttttcgctga ctgcgggttg gcgtcgatga
     6361 taaatccagc ggtatgcaaa tcttcattcc agttttgcat taacagcttc gcagtaagcc
     6421 cactgccttt ggtataggcg gtataggttg ccttatagac gccatctgcg gtttctttcc
     6481 agtctgtagt gacaccaggt ttcacgttgt cgatgctgac tgcggtattc agttgctgtt
     6541 tttgctcctt aacaggtttg tcattttcat ctctcagttc taccgtcacc tcgataggat
     6601 tcccggagag ataacggtcc ttatcaatct taattgacga gtgagtccgg gatgacgaaa
     6661 cagaaatgat attcaccacg gcgggggctt tagccgcatc cacaccgttc agctgtggca
     6721 tcagcgtcag cgtgccagac atcgctcctg tggtcaggat ctgggtatag cttccgtcac
     6781 cattatcttt ccagtcagaa agggtgatgt cctgaacacc ttcgtgacgc gtcgagagca
     6841 ccagcccgat aacaggatta cctgctgcat catgcgcaat aaaagtcagt gtggcggttg
     6901 aatgggaatc cgcgctcaac gtctggctac ttaacgatac cgaggaatct ttctggctta
     6961 gcgtaggagc ctgaacgact accatgctct gttcacgatt cgaaaaattg cctttgacat
     7021 cttcagcggt gacttcaatc ggccaggtgt tatcggtttc tggcgtactg gtgaaccggt
     7081 acgccggcag ggtaaccaga atatctttac ccgttgtaac cactttacca ccggcagctt
     7141 ccagagcggt ggcttcgacg ttatagcctt tcagggcata tttggtttgt agcgacgaaa
     7201 ccagtgattt cacttctcct gacttccctg tcacggggtc tgtcagggtc aggcgaacca
     7261 gttctttttt gcgatattcc agaacgatgt tgttgttgcg atccaccaga tcaaaacggc
     7321 tgcctgcaag gctacgccgt gcatcgactt cattcgggtc aagctgtttc tgcatcgcgc
     7381 ttccaggttg ccaggtaaaa tcgacggcaa aacgggtgtc attttcgccc tgtttaccct
     7441 ggcgttgctc cgcgctgaag gtcatcagcg ggaagggggt atagttaagt ccagcggtta
     7501 tggcatgagg attactttgc cgatcatctt tatcgaacag ggccacttca tcgccataat
     7561 actgttcata gaccagttta ccgccaaggt gcggccaggc gggtagccag ccttctgcgc
     7621 gtacatccca gccattggcc gggcgtgctt cataatcgtt gtccagttca ggtgcgctgc
     7681 gccagttggt cagtcgcaaa tagccgttac tgcttaattt tagatagtcg cgccagtact
     7741 ccgcgccaat gccggcgcgg gagtggtaac ggctaagatc gtggtcgaaa aagaagttga
     7801 tgcccgacat ccatgtggga gtgaaatgac gccaacccaa gccgttgtta atctgcgtac
     7861 gctcgtcagt acgatggaga gtatgctgac tgaaaaagag attatcaggc gtttcatacc
     7921 acggatggag aaaatcgaac tgggagttct tcaggctaaa atcttcatcc acgcccagcg
     7981 tgattcttgc ggtaccgaag cggcttaacc agtctgtcat tgcgcctgaa gcctgagaag
     8041 aggcccatcc acgcgccata tttgccgctt gctcgctgtt catatcctcg gcgagcagag
     8101 acccgatttg ctgtgaagta ctggctatct gttgctcaag gttgccactg ctattacccg
     8161 gtggcggggt taaattattt tcactaactt gtgccgggac atccagttca tcaccctggc
     8221 ggacattatc aaaacctcga gcaaacgtac gaaactggtt gagtttgcgt aactcagcca
     8281 ccgaaatacc gaaacgttcg gcaacgattt gggccgattc cagcgctcca agggtgtagg
     8341 gcaccgtatt ggcatttgca atggcaattt gtgcaggaac tggttgttgg gttgcggcgt
     8401 ttaccacacc ttgtgctgcc gcagccatag ggaacacaag ttgagttatc aggcagatac
     8461 ccgcagttaa gcggcgtagt ttaattccca tcccgcataa gatttgtcgg tcatttattt
     8521 cttctccact tctcttcttc gtagccatgc ttaccttccc tgaacgagct aacataaatg
     8581 cgccctttct agcacagcga aaaa
//
LOCUS       DBFIFI010000182         8587 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.182, whole genome shotgun sequence.
ACCESSION   DBFIFI010000182 DBFIFI010000000
VERSION     DBFIFI010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.182"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(16..762)
                     /gene="dsbG"
                     /locus_tag="N7B39_003865"
     CDS             complement(16..762)
                     /gene="dsbG"
                     /locus_tag="N7B39_003865"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308670.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HGU2423761.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQEYEASGGKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            1134..1697
                     /gene="ahpC"
                     /locus_tag="N7B39_003866"
     CDS             1134..1697
                     /gene="ahpC"
                     /locus_tag="N7B39_003866"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HGU2423762.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            1826..3391
                     /gene="ahpF"
                     /locus_tag="N7B39_003867"
     CDS             1826..3391
                     /gene="ahpF"
                     /locus_tag="N7B39_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HGU2423763.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(3512..3940)
                     /gene="uspG"
                     /locus_tag="N7B39_003868"
     CDS             complement(3512..3940)
                     /gene="uspG"
                     /locus_tag="N7B39_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HGU2423764.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            4161..5399
                     /gene="ybdR"
                     /locus_tag="N7B39_003869"
     CDS             4161..5399
                     /gene="ybdR"
                     /locus_tag="N7B39_003869"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HGU2423765.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRLQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            complement(5403..5492)
                     /gene="yldA"
                     /locus_tag="N7B39_003870"
     CDS             complement(5403..5492)
                     /gene="yldA"
                     /locus_tag="N7B39_003870"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HGU2423766.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(5630..6040)
                     /gene="rnk"
                     /locus_tag="N7B39_003871"
     CDS             complement(5630..6040)
                     /gene="rnk"
                     /locus_tag="N7B39_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HGU2423767.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(6270..7076)
                     /gene="rna"
                     /locus_tag="N7B39_003872"
     CDS             complement(6270..7076)
                     /gene="rna"
                     /locus_tag="N7B39_003872"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HGU2423768.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQVSIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            complement(7190..>8587)
                     /gene="citT"
                     /locus_tag="N7B39_003873"
     CDS             complement(7190..>8587)
                     /gene="citT"
                     /locus_tag="N7B39_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HGU2423769.1"
                     /translation="LIPVPDGMPPQAWHYFAVFVAMIVGMILEPIPATAISFIAVTIC
                     VIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSSTTVWLVFGAFIFALGYEVSGLGR
                     RIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNTARTGGTVFPVIKNLPPLFKSFP
                     NDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVLGLEFVSKIAGIQISWLQWFLCF
                     LPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDELKTMGALTRREWTLIGLVLLSL
                     GLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITRYNSAWNTLVNLATLVVMANGLT
                     RSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAHYLFASLSAHTATMLPVILAVGK
                     GIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIYGCGYVKSKDYWRLGAIFGVIYI
                     SMLLLVGWPILAMWN"
BASE COUNT         2234 a         2093 c         2187 g         2073 t
ORIGIN      
        1 tccatgaatt accttttatt tatttcccat aatgatatta agcgttttct gatcgggcaa
       61 ccccacggcc tgttgcagcg tattttcctt actcatgtaa tagatagccg gagtgacatt
      121 tgcccccaga tcgtccatga gtttctcgtt gtcgcttaac actttcattt gctctgtact
      181 tacgtttgca ggcacgttta gcttaagctt gccaccagag gcttcatatt cttgccaggt
      241 ttttgcggga tctttggagg caagaattgc cgcggctgtc gccggacttt ctggtttgat
      301 aaccccaacc aacaatgttc tcaactgtac tttgccagaa tctacccacg ggcgcgcctg
      361 ctgccagaac tgtttacaat atgggcagaa cggatcggcg aagacgtaga caatgaccgg
      421 cgcatctttt ttaccgtcga ggagccagtg ggattgttcc atccgttgcc acatttcgcg
      481 tccggctggt gcgtaaattt ctttttcgat aagtgtgtta ctcaggtttt cacctttctc
      541 gttgtacatg taaccagaga tagcgtgctt accatctgga gtcaggtaga tggtgacgcc
      601 catatcctga tactttccga gataaccttt cattcctccg ggggcatcga acgttttgat
      661 gattgtaatg ccctgttttt caatcgcttt tactggagca ggaagttcct ctgcgaaggc
      721 gattgcagga agcagagcca gtaaaagtat cttttttaac attaatttgt ccttttcagt
      781 cagtgcaaaa gtcgagtaaa aggcataacc tatcattgtc ataggtaaga gcttagatca
      841 ggtgattgcc ctttgtttat gagggtgttg taatccatgt cgttgttgca tttgtaagag
      901 caacaacgca gcctgcagac tggcgctgaa gataccaaag ggtagttcag attacacggt
      961 cacctggaaa gggggccatt ttacttttta tcgccgctgg cggtgcaaag ttcacaaagt
     1021 tgtcttacga aggttgtaag gtaaaactta tcgatttgat aatggaaacg cattagccga
     1081 atcggtaaaa attggttacc ttacatctca tcgaaaacac ggaggaagta tagatgtcct
     1141 tgattaacac caaaattaaa ccttttaaaa accaggcatt caaaaacggc gaattcatcg
     1201 aaatcaccga aaaagatacc gaaggccgct ggagcgtctt cttcttctac ccggctgact
     1261 ttactttcgt atgcccgacc gaactgggtg acgttgctga ccactacgaa gaactgcaga
     1321 aactgggcgt agacgtatac gcagtatcta ccgatactca cttcacccac aaagcatggc
     1381 acagcagctc tgaaaccatc gctaaaatca aatatgcgat gatcggcgac ccgactggcg
     1441 ccctgacccg taacttcgac aacatgcgtg aagatgaagg tctggctgac cgtgcgacct
     1501 tcgttgttga cccgcagggt atcatccagg caatcgaagt taccgctgaa ggcattggcc
     1561 gtgacgcgtc tgacctgctg cgtaaaatca aagcagcaca gtacgtagct tctcacccag
     1621 gtgaagtttg cccggctaaa tggaaagaag gtgaagcaac tctggctccg tctctggacc
     1681 tggttggtaa aatctaagtt ccgcaatcgc gtaattaatc gggtgctgcg gcacccgatt
     1741 ttcttccccg caccatgatg caagctgcat ccaggtagcc gcagaggccg cttgcatgat
     1801 gatgtttaaa gcccaggaga taaacatgct cgacacaaat atgaaaactc aactcaaggc
     1861 ttaccttgag aaattgacca agcctgttga gttaattgcc acgctggatg acagcgctaa
     1921 atcggcagaa atcaaggaac tgttggctga aatcgcagaa ctgtcagaca aagtcacctt
     1981 taaagaagat aacagcttgc cggtgcgtaa gccgtctttc ctgatcacca acccaggttc
     2041 taaccagggg ccacgttttg caggctcccc gctgggccac gagttcacct cgctggtact
     2101 ggcgttactg tggaccggtg gtcatccgtc gaaagaagcg cagtctctgc tggagcagat
     2161 tcgccatatt gacggtgatt ttgaattcga aacctattac tcgctctctt gccataactg
     2221 cccggacgtg gtacaggcgc tgaacctgat gagcgtattg aacccgcgca tcaagcacac
     2281 tgcaattgac ggcggcacct tccagaacga gatcactgat cgtaacgtga tgggcgttcc
     2341 ggcagtgttc gtgaacggga aagagtttgg tcagggccgc atgactttga ctgaaatcgt
     2401 tgccaaaatt gatacgggcg cggaaaaacg tgcggcagaa gagctgaaca agcgtgatgc
     2461 ttatgacgta ttaatcgtcg gttccggccc ggcgggtgca gcggcagcaa tttactccgc
     2521 acgtaaaggc atccgtaccg gtctgatggg cgaacgtttt ggtggtcaga tcctcgatac
     2581 cgttgatatc gaaaactaca tttctgtacc gaagactgaa gggcagaagc tggcaggcgc
     2641 actgaaagtt cacgttgatg aatacgacgt tgatgtgatc gacagccaga gcgccagcaa
     2701 actgatccca gcagcagttg aaggtggtct gcatcagatt gaaacagctt ctggcgcggt
     2761 actgaaagca cgcagcatta tcgtggcgac cggtgcaaaa tggcgcaaca tgaacgttcc
     2821 gggcgaagat cagtatcgca ccaaaggcgt gacctactgc ccgcactgcg acggcccgct
     2881 gtttaaaggt aaacgcgtag cggttatcgg cggcggtaac tccggcgtgg aagcggcaat
     2941 tgacctggcg ggtatcgttg agcacgtaac gctgctggaa tttgcgccag aaatgaaagc
     3001 cgaccaggtt ctgcaggaca aactgcgcag cctgaaaaac gtcgacatta ttctgaatgc
     3061 gcaaaccacg gaagtgaaag gcgacggcag caaagtcgtt ggtctggaat atcgagatcg
     3121 tgtcagcggc gatattcaca acatcgaact ggccggtatt ttcgtgcaga ttggtctgct
     3181 gccgaacacc aactggctcg aaggcgcagt cgaacgtaac cgcatgggcg agattatcat
     3241 tgatgcgaaa tgcgaaacca acgtgaaagg cgtgttcgca gcgggtgact gtacgacggt
     3301 tccgtacaag cagatcatca tcgccactgg cgaaggtgcc aaagcctctc tgagtgcttt
     3361 tgactacctg attcgcacca aaactgcata agaagaagta agattcacct gcaattgctt
     3421 agccgccggg gtcaaacctg gcggcttttt tatggcatta aaaagccctg ccgggatgtt
     3481 ccaggcaggg cggcataaat acttttatct gttagcgaac gaccagtaca ggcagattgg
     3541 cgtggcggat cacgctcgag gcgttagaac ctaacagatg ggtcgaaatc gatgggttgc
     3601 gagaaccaat aactacaaca tcagccccca gttcttctgc taactcattg acttcatccc
     3661 gcacgctacc aaaacggaca tgttgtttaa tgcgggaagg atcgatggtg aagtggctga
     3721 ccatcgtttg caaacgttct tctgcttcat gttgcagatg ctcttcaaaa cgacgcacat
     3781 cagcggcaaa acggtgcagg ctcaggctgg ctgacccggg tagtacgtga agtagatgaa
     3841 taactccgtc atcctgggcg aggaattcag cgtggcgaac agctttgtcg ctcaattcca
     3901 tttcaaatac atcaactggc ataatgattg tcttatacat aaccctttct ccctgttaat
     3961 catgaacaaa tcattcgcca tgattataat atttatccct gatatttgtc tggttctttt
     4021 tccttacgaa ctgtttctgt gatgaatata ttctcactga acaccaggaa ttctcccaaa
     4081 acctgcggta ccgcccgttt tcccgctgtg atagctaccc ttaaagactg actctttttt
     4141 gaactgtctc tggaggttgc atgaaagcat tgacttatca cggcccacat cacgttcagg
     4201 tagaaaatgt tcccgatccg ggcattgaac aggcagatga tattattctg cgtataacgg
     4261 caacggcgat ctgtggctct gacctccatc tttatcgagg caaaataccc caggttaaac
     4321 atggcgatat ttttggtcat gaatttatgg gggaagtagt tgaaaccgga aaggatgtaa
     4381 aaaatttgca aaaaggcgac cgggtggtaa ttccgttcgt cattgcttgt ggcgactgtt
     4441 ttttctgtcg actgcagcaa tatgctgcct gcgaaaatac caatgcgggt aaaggcgctg
     4501 cgctcaataa aaaacagata ccagctcccg cggcattgtt tggttatagt cacctgtatg
     4561 gtggcgttcc tggtggacag gcggagtatg tccgtgtccc taaagggaat gtggggccgt
     4621 ttaaagtacc gcctttgctt tcagatgata aagccctttt cctttctgat attctgccaa
     4681 cggcatggca ggcagcaaaa aatgcgcaga tccaacaagg ttcaagcgtt gctgtctatg
     4741 gtgccggtcc tgtgggattg ttgacaatcg cctgtgcacg gttgctcggt gcggaacaga
     4801 tttttgttgt tgatcatcat ccctaccgct tgcgtttcgc cgctgaccgc tacggcgcga
     4861 tcccgattaa tttcgatgaa gatagcgatc cggcacagtc aattattgaa caaacggcag
     4921 gtcaccgggg cgtggatgca gtaatagacg ccgtcggttt tgaagcgaaa ggcagcacca
     4981 cggaaacggt gctgactaac ctgaaactgg agggcagcag cggtaaagcg ttgcgtcagt
     5041 gtattgcggc ggtcaggcgt ggcggcattg ttagcgtacc gggcgtctac gctggattta
     5101 ttcacggttt cctgtttggc gacgcctttg ataaagggtt gacgtttaaa atgggacaga
     5161 cccacgttca cgcatggctg ggagaattat taccgttaat tgagaaagga ttactgaaac
     5221 cagaagaaat tgttacccac tatatgccgt ttgaagaggc cgcccgggga tatgagatct
     5281 ttgaaaagcg tgaagaggag tgccgtaagg tgattctggt acccggtgca caaagcgcag
     5341 aggcggcgca gaaggcggtt tcaggtctgg tgaatgcgat gccgggggga acaatatgag
     5401 cgtcaggagt ggttttcgag gtaaaggaca gccatgacga taatcgccgc cataatcaga
     5461 aatcctatca ggatgtaaaa tgcttctgcc atggttattc ccacaaacga aacggaataa
     5521 ttttgcagca aaagtgaaca gtgagaacca ggaaaaattg ctgattttgc ataaagagga
     5581 tgcgagtgca tcctctgggc aaagcgagtt atcgcttgta cagatgggat taaagcaggt
     5641 agtcgccagc agcttctggc tggtattcga gttccagcac ttcaaggtgg gttgcaacgc
     5701 cgcccggaag ttcccagtga atggaatcgc caacgcgcag tcccagcagt gcggcaccta
     5761 ccggagccat aacggaaagc tgagtattgc tatcggtcat ttttgccgga tacaccagcg
     5821 tgcgcacacg cacttcgcca tcgctaagat tgcggaattt aacccggctg ttcattgtca
     5881 ccacgtcgtg tggcatctct tctggcgaac acatttgggc gcgatccaac tctgcgttta
     5941 acgcgtcggc gattggcaaa ccagcatagg cgggttgctc cagcagaata tcgatgcgtt
     6001 cggcatccag gtcgttaatg atgatagttg gtctggacat ttttactcca tgtcgtcggt
     6061 actgcgagtg tcgcagataa acatacccaa aagaaaaccc tcaccgtcag gcggcgaggg
     6121 tttaactcac atgatgatac tgactgttgc tcactctttg aagtgatttg cgtcacattc
     6181 agggaattcc tcaatgcagc gcattatgta taattcttaa tcgtcatggt ttatgaaaga
     6241 cgaattagcg tgttttgtaa atcagaggat taataacccg ctttatcaat cacaaaggtt
     6301 ttgccacagt tacctgggtg tggttgtggc aagaatgagt ttgcagaaag cggagcgttg
     6361 atggcgtcag ctttgatcga gacctgaatt tcagtcaaat acgcagggtt accctggcac
     6421 gttagcttaa ctgctttcac gttctctttt ccccagcttt tggcaaaggc ggcgtcaaag
     6481 tcacggcggc tcactgtttt accgtaatta tccgcaagga attttccggc ttcgctctct
     6541 tttatctctt gattcaggcg taccatcgta ccgaagtatg cgtccggatc aaaaccaaag
     6601 caggcaccgt gtttggcata ttcgtagcgt tccaggcagg aacgtccgcc agctcctggc
     6661 atgacttcac ttagtttagc ggccgtttcc agtgataatc cggtttccgg cgatgaacac
     6721 atccggctgg cgcgcgcttc tggtagattc gggattgggc gagtagcgca accgaagcgc
     6781 atccagcggc gttcatcaac accacgggca gcaaccgatt taggcaatcc tggccacaga
     6841 ccatgtacgg tcagaaaatc agctttgttg gtcgtttcgg tttgcaggcg acattcatct
     6901 cgttcgttac gatttcgatc gtgttgactc tggcaaaatc cggtttgcca ggagagggcc
     6961 aggacatagc gatcaaaatc gccatactgt tttgcctgca acgctacggc gttggcagaa
     7021 gagaagggaa gcagagaaac cgcgagcaac gcggcgttac gccagaatgc tttcataatg
     7081 ggtgtggaac tcatacatac actgaatact atctattaaa tcataaaaaa gcccgccagg
     7141 tgggctaacc tggcgggcgt gatgatttat tcagcgtttg gcgaacgtat tagttccaca
     7201 tggcgagaat cggccagcca accaacagca acatagagat gtaaatcacc ccgaagattg
     7261 cgccaagacg ccagtaatct tttgatttca catagccaca gccgtaaata atcaccccag
     7321 gaccggttgc atacggcgtc agacagccca tgataccgat agacagcacc agcaggatac
     7381 acagttgttc cattggtacg cccggaatac ctttaccgac ggccagaata accggcagca
     7441 tggttgcggt gtgcgcagac aggctggcaa acaggtagtg tgcaaagtag aacaccagaa
     7501 ccagtacaat caccgttgcg tttggtgaga atccttccag gtgcgtactc atggtaccgg
     7561 cgaaccagtc aataaaacca gaacgagtca ggccgttagc catcacaacc agagttgcca
     7621 ggttgaccag cgtgttccat gcgctgttat agcgggtaat gtctttccac ggcacaacgt
     7681 gcagagccag cattagcgaa actgccagca gaccaaccgc agtagcatta atgacttcac
     7741 tgccaaatac ccacaaacct aagctgagca atacaaggcc aatcagcgtc cactctctgc
     7801 gtgtcagcgc acccatggtt tttagttcat cacccgccca ggttgccact tcttcactgt
     7861 gtgtgatttc cggtttgtac agcacgtagg aaagccacgg cgcaatgata agcaagataa
     7921 ccccaaccgg caggaagcag aggaaccact gcaaccagct aatctggata ccggcaattt
     7981 tgctgacgaa ctccagaccc agcacgtttg gtgccgcacc ggtgacaaac atggacgaac
     8041 tcagactggt actaatgacc atcatccaca tcaaatagcc gccaatacga cgcgcggacg
     8101 gatcgttcgg gaatgattta aacaacggcg gcaggttttt aatgaccgga aaaaccgtac
     8161 ccccggtacg cgcggtgttg gacggtgtaa acggtgccag cagaatgtcg ataatgacaa
     8221 tcgcataacc caacgtcagc gtgcgtttgc ccatgaattt caccaggaaa agggcaatgc
     8281 gacgacctaa cccggaaact tcatacccta atgcaaaaat aaatgcgcca aataccagcc
     8341 ataccgtagt gctggaaaaa ccagccaggc cccatttcag cgcctgtttt tgcgcattaa
     8401 acgctgggtc agctaattct ttggcatcaa agagcaggta attactgcca ataacgcaaa
     8461 tagtaaccgc aataaaactg atcgctgttg ccggaattgg ctcgaggatc atgccgacaa
     8521 tcattgccac aaacacagcg aagtaatgcc atgcctgcgg cggcataccg tcggggacag
     8581 ggataag
//
LOCUS       DBFIFI010000183         8559 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.183, whole genome shotgun sequence.
ACCESSION   DBFIFI010000183 DBFIFI010000000
VERSION     DBFIFI010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8559
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.183"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(6..2645)
                     /locus_tag="N7B39_003874"
     CDS             complement(6..2645)
                     /locus_tag="N7B39_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HGU2423770.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPSVKLVGEFTMPLMPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANAGPMTLRLDKDTADAVAKDNMTAGTAINWLRYMEIS
                     RSSTRINLDNLGVLTMQANISGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQV
                     WLEQNAQLPETGCPSGKECEEKQ"
     gene            2853..3842
                     /gene="ldhA"
                     /locus_tag="N7B39_003875"
     CDS             2853..3842
                     /gene="ldhA"
                     /locus_tag="N7B39_003875"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HGU2423771.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            3953..4375
                     /gene="hslJ"
                     /locus_tag="N7B39_003876"
     CDS             3953..4375
                     /gene="hslJ"
                     /locus_tag="N7B39_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HGU2423772.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTCDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(4372..4638)
                     /locus_tag="N7B39_003877"
     CDS             complement(4372..4638)
                     /locus_tag="N7B39_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HGU2423773.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            4912..8436
                     /gene="nifJ"
                     /locus_tag="N7B39_003878"
     CDS             4912..8436
                     /gene="nifJ"
                     /locus_tag="N7B39_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HGU2423774.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPAFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT         2119 a         2260 c         2142 g         2038 t
ORIGIN      
        1 tattttcatt gtttttcctc acactctttt cctgatgggc agccagtttc cggtagctgg
       61 gcattctgct ccagccatac ctggagatta tcgccaaagc gtaaactgcg ccataacgta
      121 aaaatattct cttcatggtg ataattaagg tttaccgtac cgcttttacc atcgacgcga
      181 ctggtgcccg aaatgttggc ctgcatggtt aacacaccga gattatctaa attaatcctt
      241 gttgatgagc gactgatttc catataacgc aaccagttga tggcagttcc agcggtcata
      301 ttgtctttgg ccaccgcatc tgcagtgtct ttatccagac gcagcgtcat cggccccgcg
      361 tttgccaacc agccatcttt gacaatccat ttttcattgt tcagccatag tggcaacgcc
      421 ccactaaatg ccccagacat agcaaactgt ttcggattaa ccgcgctaac cagttcgctg
      481 gatgagagat tattcagccg caacagcgcc gggtcatgtt gcggcatacg taattgtttc
      541 atcagcacgt taccgcctaa cacatcgacg ctaacatcag tcaacagcaa tggttcttct
      601 tcagtccacg gataacgccc ttgcaaatca gccgtaatat ttttcgctgt caccagatta
      661 atcacttcgg caattcgcaa cgtaacgggg ccgcgagtcc ccagatgcca ggctccatcg
      721 gcaaaacgga aaggcaggac aaaatcgaca ccgttaacct gattatctgg catccaggca
      781 ctaccgcctt tcaacacgcc gtgccctccc gcgcggaatc cttgttcagg cgcagcagaa
      841 aatgcaacct gagcatacag ttcaccatcg cgtaagttca tcttccagtc gggtggcacc
      901 agcggctgga atacagtcag tgattgttta ggccaccagg cgttaccgcg cagacgaata
      961 ccgtcccagc gaccatttac ccgaaccggg ccaatctcac cagcatgtaa atcgccttta
     1021 aagaggaaat acgtcggatc gcgcccatcg acgctaaatt ttaaggttga tggcggtaac
     1081 acactgccgc cagtgaacag cgtttgcccg gcgtccagtg acagcgcgcc gctgaaggag
     1141 ggatgctgtg cgtcacgtac ccagacgacg ggcttgtcga gaattaatcg cggcttttct
     1201 accgtcatcg taccgtattg taactggtcg aaaccggtgg agagatcggt cagcgtaatc
     1261 gtgctgtcat gccactcacc ttttcctgcg acatcccagg tggcattcat cggtgtaaaa
     1321 ctcccttttc cccagtagcg ccactgccag cggccagcgt cagggagaaa atcattcgcc
     1381 agcccatcca tatgcagcac gaaatcgccc agttcatttt catgcgcctg caaaatggcc
     1441 tgcaaacgtc cgtcaacacc acgttgggtg acttttacac ccgctaaagg ccagcggatt
     1501 tcatcgatgt ccagcgaatc gatgactctt ccctttgaac gaagtaacgc gcctggctca
     1561 aaggtcagcg ttgggtcaga cagacttcca cttagctgcg caggtaaacg ggcatataaa
     1621 atgaggtccg cctgtttcgc ttcaccggtc agctgcagag gcagctggct gttatccatg
     1681 cttaattttc ctgggccaaa attaagcacc gcgttgccct tacccgcttg cccctgggtc
     1741 agcacactca gtcgtccgct gaccagagcg ttctcaagcc ctgcctgcca gttgtcgact
     1801 ttgacaccca gtcggccact caaaggaaaa cctgcatacg gccagctcca gcgaccatcg
     1861 cttacggtca attgttgacg agtaatttgc cacggcaaat cgagcaacgg atcgccgtta
     1921 tcccgtgcca gcacgatcaa ttgcccgcta ttttcctgcc agtccagctc ggcatccacc
     1981 agtgacggtt cctgcggcaa gtttaacgtc gcagtagcat gcccacttac aggaagtcca
     2041 tccggcatga gcggcatagt aaattccccc accagtttaa ccgacggctg attttcaaac
     2101 gcgacgacat ccagttcgct gactgtaagt tgttgccctt tcagctggcc ttgaaattta
     2161 actttttcgc cctgataacg cagttgctgg atatcagagg ttaatgcgag agagagtttt
     2221 ccctgccatt cctgccaggg agaaaaaatc agtttatcaa tattgatcca ggtgttaggc
     2281 agcatggact gccattgcgc gagggttttt ggagcagctg gcgattgctc cgtctgcggc
     2341 aatttcgcca gacaagcaga atcaagttct accgtgccga cgttcaataa ccagcggctg
     2401 ggatgtgaaa ggctggcgtt ggtgatatgc gcaagctgac aatctcccac cagataacgg
     2461 agatcgggaa tgattaaacc tttacgcgta atgcgtgggc tttcatctaa tgcaatacgt
     2521 gtcccgagcg gtagccagat gcccgccagc gtgggaaccc acagcccgag cgtcatcagc
     2581 agcgtcaacg gcacaagaat aatcagtaat aacagcgcga gaacggcttt atatttaccc
     2641 agcatgggta gttaatatcc tgatttagcg aaaaattaag cattcaatac gggtattgtg
     2701 gcatgtttaa ccgttcagtt gaaggttgcg cctacactaa gcatagttgt tgatgaattt
     2761 ttcaatatcg ccatagcttt caattaaatt tgaaattttg taaaatattt ttagtaactt
     2821 aaatgtgatt caacatcact ggagaaagtc ttatgaaact cgccgtttat agcacaaaac
     2881 agtacgacaa gaagtacctg caacaggtga acgagtcctt tggctttgag ctggaatttt
     2941 ttgactttct gctgacggaa aaaaccgcta aaactgccaa tggctgcgaa gcggtatgta
     3001 ttttcgtaaa cgatgacggc agccgcccgg tgctggaaga gctgaaaaag cacggcgtta
     3061 aatatatcgc cctgcgctgt gccggtttca ataacgtcga ccttgacgcg gcaaaagaac
     3121 tggggctgaa agtagtccgt gttccagcct atgatccaga ggccgttgct gaacacgcca
     3181 tcggtatgat gatgacgctg aaccgccgta ttcaccgcgc gtatcagcgt acccgtgacg
     3241 ctaacttctc tctggaaggt ctgaccggct ttactatgta tggcaaaacg gcaggcgtta
     3301 tcggtaccgg taaaatcggt gtggcgatgc tgcgcattct gaaaggtttt ggtatgcgtc
     3361 tgctggcgtt cgatccgtat ccaagtgcag cggcgctgga actcggtgtg gagtatgtcg
     3421 atctgccaac cctgttctct gaatcagacg ttatctctct gcactgcccg ctgacaccgg
     3481 aaaactacca tctgttgaac gaagccgcct tcgatcagat gaaaaatggc gtgatgatcg
     3541 tcaataccag tcgcggtgca ttgattgatt ctcaggcagc aattgaagcg ctgaaaaatc
     3601 agaaaattgg ttcgttgggt atggacgtgt atgagaacga acgcgatctg ttctttgaag
     3661 ataaatccaa cgacgtgatc caggatgacg tattccgtcg cctgtctgcc tgccacaacg
     3721 tgctgtttac cgggcaccag gcattcctga cagcagaagc tctgaccagt atttctcaga
     3781 ctacgctgca aaacttaagc aatctggaaa aaggtgaaac ctgcccgaac gaactggttt
     3841 aatcttgccg ctcccctgca atccagggga gctgattcag ataatcccca atgacctttc
     3901 attctctatt cttaaaatag tcctgagtca gaaactgtaa ttgagaacca caatgaagaa
     3961 agtagccgcg tttgttgcgc taagcctgct gatggcggga tgtgtaagta atgacaaaat
     4021 tgctgttacg ccagaacagc tacagcatca tcgctttgtg ctggaaagcg taaacggtaa
     4081 gcccgtgacc tgcgataaaa atccgccaga aatcagcttt ggtgaaaaaa tgatgatttc
     4141 cggcagcatg tgtaaccgct ttagcggtga aggcaaactg tctaatggtg aactgacagc
     4201 caaagggctg gcaatgaccc gtatgatgtg cgctaacccg cagcttaatg aactcgataa
     4261 caccattagc gaaatgctga aagaaggtgc acaagtggat ctgaccgcga accagttaac
     4321 gctggcgacc gcgaaacaga cattaactta taagctggcg gatttaatga attaatagct
     4381 gccacagctc ccggcggcaa gtgactgttc gctacagcgt ttgccgttgg gtaatgcaca
     4441 catcccaatc gccgtaccat ccagttgacg ggcaacagaa agcgaaccgc cgatcattgc
     4501 acaatttgct tctccactac tggacatcga cgcttttaaa cctggcgcta cgtgcgccgc
     4561 agtcgcctgc tgaacaggct cactactaca cgccgacaac aataaagcgg cacaccctac
     4621 ccaaaacgct gctcgcatct cttcttcctc tgatcttcaa gccaaacgac accgccataa
     4681 ataataggca gcacagaggg cgacgtcgag agctgtcctg cgcgttgccc cgcattttta
     4741 cttttttatg gctatttttt tgccctctgt ttgatcaaaa cattcattac gctgatgtgg
     4801 gggacacaaa agcgaaaatg cagaagaaag ccatttgcta aaattgaaag attactactg
     4861 ggcgcgcagc aatttcgtgc gcccctcatt tgcgcaatgt aagggtgtca tatgattact
     4921 attgacggta atggcgcggt tgcttcggtg gcgtttcgca ccagtgaagt tatcgccatc
     4981 taccctatta cccccagttc cacgatggca gaacaggctg atgcctgggc cggaaacggc
     5041 ttgaagaacg tttggggaga cacaccacgc gtggttgaaa tgcagtcgga agcgggtgct
     5101 atcgctaccg tgcatggcgc tttgcagacg ggtgcccttt caacatcgtt tacgtcatcg
     5161 cagggtttgc tgctgatgat cccgacgctg tacaaactgg caggcgaact cacaccgttt
     5221 gtcctgcatg tagcggcacg taccgttgcc acacatgcac tctctatttt tggcgatcat
     5281 tccgacgtca tggcggtgcg ccagacgggt tgcgcgatgt tgtgtgcggc aaacgtccag
     5341 gaagcgcaag actttgctct catttcgcac atcgcgacgc tgaaaagccg cgtgccattt
     5401 attcatttct ttgatggttt ccgtacgtcc cacgaaatca ataaaattgt cccgctggcc
     5461 gatgacacta ttcttgatct catgccgcag gccgaaattg atgctcatcg cgcccgggcg
     5521 ctcaacccgg aacatcccgt gatccgcggt acgtcagcca atcctgacac ttatttccag
     5581 tctcgcgaag ccaccaaccc atggtacaac gcggtctatg accatgttga acaggcgatg
     5641 aatgatttct ctgccgcgac aggtcgtcag tatcagccgt ttgaatatta cgggcatccg
     5701 caagcggaac gggtgattat cctgatgggc tctgccattg gcacctgtga agaagtggtt
     5761 gatgaattgc taacccgtgg cgaaaaagtt ggcgtgctga aagttcgcct gtaccgtccc
     5821 ttctccgcca aacacttgct gcaagctctg ccgggatccg tacgcagcgt ggcggtactg
     5881 gacagaacca aagaacccgg tgcccaggca gaaccgctct atctggatgt aatgaccgca
     5941 ctggcagaag cctttaataa tggcgagcgc gaaactctgc cccgtgtcat tggtgggcgc
     6001 tatggtcttt catccaaaga atttggcccg gactgtgtac tggcggtatt tgccgagctc
     6061 aacgcggcta aaccgaaagc gcgctttacg gttggtattt acgatgatgt gaccaatctg
     6121 tcactgccgt tgccggaaaa caccctgcca aactcggcga aactggaagc cttgttttat
     6181 ggccttggta gtgatggcag cgtttccgcg accaaaaaca atatcaagat tatcggtaat
     6241 tccacgccgt ggtacgcaca gggctatttt gtttacgact ccaaaaaggc gggcggcctg
     6301 acggtttctc accttcgcgt gagcgaacag ccgattcgtt ccgcttatct catttcccag
     6361 gctgattttg ttggctgcca ccagttgcag tttatcgata aatatcagat ggctgagcgt
     6421 ttaaaacctg gcggcatttt cctgctcaac acgccgtaca gcgcagatga agtgtggtcg
     6481 cgcttgccgc aggaagttca ggccgtgcta aatcagaaaa aagcgcgctt ctatgtgatt
     6541 aacgcggcga aaatcgcccg cgaatgtggc ctggcggccc gtattaatac cgtcatgcag
     6601 atggcttttt tccatctgac acaaattctg cctggcgata gcgccctcgc agaattgcag
     6661 ggtgcgattg ccaaaagtta cagtagcaaa ggtcaggatc tggtggaacg caactggcag
     6721 gctctggcgc tggcgcgtga atccgtagaa gaagttccgt tgcaaccggt aaatccgcac
     6781 agcgccaatc gaccgccagt ggtttccgat gccgcccctg ctttcgtgaa aaccgtaacc
     6841 gctgcgatgc tcgccgggct tggtgatgcc ctccccgttt cggcgctgcc gccagacggc
     6901 acctggccga tgggcaccac tcgctgggaa aaacgcaata tcgccgaaga gatccccatc
     6961 tggaaagagg aactctgtac ccaatgtaac cactgcgtcg ccgcttgccc acactcagct
     7021 attcgcgcaa aagtggtacc gcctgaagcg atggaaaacg cccctgccag cctgcattcg
     7081 ctggatgtga aatcgcgtga tatgcgcggg cagaaatatg tcttgcaggt ggctccggaa
     7141 gattgcaccg gttgtaacct gtgcgtcgaa gtttgcccgg cgaaagaccg tcagaatcca
     7201 gagattaaag ccatcaatat gatgtcgcgc ctggaacatg tcgaagaaga gaaaatcaat
     7261 tacgatttct tcctcaacct gccagaaatc gaccgtagca aactggaacg tattgatatt
     7321 cgcacatcgc agctgattac accgctgttt gaatattccg gtgcttgctc cggttgtggc
     7381 gagacgccgt atattaaatt gctgactcag ctctatggcg accggatgtt gatcgctaac
     7441 gccaccggct gttcttcaat ttatggcggt aacctgccct ctacaccgta taccaccgat
     7501 gccaacggtc gtgggccggc atgggcgaac tctctatttg aagataatgc cgaatttggc
     7561 cttggtttcc gcctgacggt cgatcaacac cgtgtccgcg tgctgcgtct gctggatcaa
     7621 tttgccgata aaatcccggc ggaattactg acggcgttga aatcagacgc cacgccagag
     7681 gttcgtcgtg aacaggttgc agctttacgc cagcaactca acgatgttgc cgaagcacat
     7741 gaactgctgc gtgacgcaga tgcactggtg gaaaaatcaa tctggctgat tggtggcgac
     7801 ggctgggctt acgatatcgg ctttggcggt ctggatcatg tattgagttt gacggaaaac
     7861 gtcaacattc tggtgctgga tacgcaatgc tattccaaca ccggtggtca ggcgtcgaaa
     7921 gcgacaccgc tgggtgcagt aactaaattt ggcgagcacg gcaaacgtaa agcgcgtaaa
     7981 gatcttggcg tcagtatgat gatgtacggt catgtttatg tggctcagat ttctctcggc
     8041 gcgcagctga accagacggt gaaagcgatt caggaagcgg aagcgtatcc gggaccatcg
     8101 ctgatcattg cttatagccc gtgtgaagag catggttacg atctggcact cagccatgac
     8161 cagatgcgcc aactcacagc taccggcttc tggccgctat atcgcttcga tccgcgtcgt
     8221 gccgatgaag gcaaactgcc gctggcgctg gattcacgcc cgccgtcaga agcactggaa
     8281 gaaacgttac ttcacgagca acgtttccgt cgtctgaatt cgcagcagcc agaagtggca
     8341 gaacagttat ggaaagatgc tgcagctgat ttgcaaaaac gctatgactt cctggcacaa
     8401 atggccggaa aagcggaaaa aagcaacacc gattaaatgc tctggataag gattatccaa
     8461 ttctaaaaaa aagcccggac gactgttcgg gcttgtcttt ttatatgttg gaaaatcagt
     8521 ggcaatgcaa tggcccaaca gaaaataaaa tctgtgaca
//
LOCUS       DBFIFI010000184         8529 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.184, whole genome shotgun sequence.
ACCESSION   DBFIFI010000184 DBFIFI010000000
VERSION     DBFIFI010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8529
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.184"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(237..443)
                     /locus_tag="N7B39_003879"
     CDS             complement(237..443)
                     /locus_tag="N7B39_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HGU2423775.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            756..845
                     /gene="kdpF"
                     /locus_tag="N7B39_003880"
     CDS             756..845
                     /gene="kdpF"
                     /locus_tag="N7B39_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HGU2423776.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALIHAEAF"
     gene            845..2518
                     /gene="kdpA"
                     /locus_tag="N7B39_003881"
     CDS             845..2518
                     /gene="kdpA"
                     /locus_tag="N7B39_003881"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HGU2423777.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLAWLINDIPLPGTTGVER
                     VLFSALGVSNHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALVIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2541..4589
                     /gene="kdpB"
                     /locus_tag="N7B39_003882"
     CDS             2541..4589
                     /gene="kdpB"
                     /locus_tag="N7B39_003882"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HGU2423778.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSVAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVVATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAVTYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4598..5170
                     /gene="kdpC"
                     /locus_tag="N7B39_003883"
     CDS             4598..5170
                     /gene="kdpC"
                     /locus_tag="N7B39_003883"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HGU2423779.1"
                     /translation="MRGLRPALSTFIFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTDNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPNASTSVPVELVTASASGLDNNITPQAAAWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5163..7847
                     /gene="kdpD"
                     /locus_tag="N7B39_003884"
     CDS             5163..7847
                     /gene="kdpD"
                     /locus_tag="N7B39_003884"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HGU2423780.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRLAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RKLALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            7844..8521
                     /gene="kdpE"
                     /locus_tag="N7B39_003885"
     CDS             7844..8521
                     /gene="kdpE"
                     /locus_tag="N7B39_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HGU2423781.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPSHFITETG
                     IGYRFML"
BASE COUNT         1801 a         2206 c         2466 g         2056 t
ORIGIN      
        1 tcatccttgt tttatcgggt tatttgtctg cttccaggca aaaccctaca gatttataag
       61 aaagccttaa gtgagatatc cgtcagatag aaacaattag gaaaatattt atatatttca
      121 tgaatatatg tttcgattgg tataacggtg tctaaatatt cctctgaaca tcttcctgat
      181 acaaaaaacc gccagcgttt ccgctggcgg ttttcaatgt attcaaaact cagcaatcag
      241 taaaaatcac cggttgcctt ttcggcctga tccatccaaa ccggtttatc gctggttttc
      301 gaccagacgc gatgcaggta gctgtaaaag cgggcgcggt ctttccagaa aagcatgaaa
      361 ggcagcgcca gaacgcccgc tgcaaccgca taagtacggc gtaaaaagat aagccatgca
      421 ggatattctc tgtagagttc catacttttc tccccctttt gtatctaccc ggtgaatagc
      481 accggaaaaa tgaatttgtt tatctgatga aaatagtacc gccttttgtg taattttact
      541 actcatccga ccacttattt ttgcttattg atggtttatt tacattcatc ctgtaattaa
      601 gttacacaaa agttaaatta atactaaaca ttagttaaat catggctttt gccattttta
      661 tacttttttt acaccccgtc cgcagatttt tgcgaaatct ttgcagccag aattctaccc
      721 ttccggtatc acttttaggc cactggaggt gcactgtgag tgcaggcgtg atagccggcg
      781 tgttgctggt gtttttatta ctgggttatt tggtttatgc cctgatccat gcggaggcat
      841 tctgatggct gcgcaagggt tcttactgat tgccacgttt ttactggtat taatggtgct
      901 ggcgcgtccc ttaggtagcg ggctagcgtg gctgattaat gatattcctc ttcctggtac
      961 gacgggcgtt gaacgcgtac tctttagcgc gcttggcgtc tctaaccatg agatgaactg
     1021 gaagcagtat ctctgtgcca ttctcggcct gaacatgctg gggctggcgg tgctgttttt
     1081 tatgttgctc ggtcagcact atctgccgct taatccacag cagttgccgg ggctgtcgtg
     1141 ggatctggcg ctgaataccg ccgtcagctt tgtcacgaat accaactggc aatcttatag
     1201 cggtgaaacc acgttgagct atttcagcca gatggcgggc ttaacggtgc aaaactttct
     1261 ttctgccgcc agcgggattg cggtgatttt tgccctcatt cgtgcattta cccgccagag
     1321 catgagcaca ctcgggaatg cctgggtcga tctgctacgc atcacgttat gggtgctcgt
     1381 ccctgtggcg ttggtgatcg cactgttttt tattcaacaa ggtgcgctgc aaaactttct
     1441 gccctatcag gcagtgaata ccgttgaagg agcgcaacag ctgttaccaa tgggtccggt
     1501 ggcttctcag gaagcgatca agatgctcgg cactaacggc ggtggtttct ttaatgccaa
     1561 ctcgtcgcat ccgtttgaaa acccaaccgc actgaccaac ttcgtgcaga tgctggcgat
     1621 cttcttgatc ccaacagcgc tgtgctttgc ctttggtgaa gtgacgggcg atcgccgtca
     1681 ggggcgcatg ttgctgtggg cgatgtcagt gatttttgtc atctgcgtag gcgtggtgat
     1741 gtgggcagaa gttcagggta atcctcatct gctggcgctc ggcgcagaca gcagcatcaa
     1801 tatggaaggt aaagagagcc gtttcggcgt gctggtcagt agcctgtttg cggtcgtgac
     1861 tacggcggcg tcctgtggcg cggtgattgc aatgcatgat tcgtttaccg ctctcggtgg
     1921 catggtgccg atgtggctga tgcaaattgg tgaagtggta ttcggcggtg tcggttctgg
     1981 tctttacggc atgatgctgt ttgtcctgct ggcggtgttt attgccgggc tgatgattgg
     2041 tcgtacaccg gaatatctgg gtaaaaaaat cgacgtacgt gagatgaaac tgactgcact
     2101 ggcgattctg gtcaccccga cgctggtgct gatgggcgcg gcgctggcaa tgatgaccga
     2161 cgccggacgt agcgccatgc tcaaccctgg cccgcatggt tttagcgaag tgctgtacgc
     2221 cgtgtcatcc gccgctaaca acaacggcag cgcctttgcc ggattaagcg ccaactctcc
     2281 gttctggaac tgtttactgg cgttctgcat gtttgtcggt cgcttcgggg tgattatccc
     2341 ggtgatggca attgccggtt cgctggtgag taaaaagagc caacccgcca gctccggcac
     2401 gctgccaacg cacggcccgc tgtttgttgg cctgttaatc ggcaccgtgt tgctggttgg
     2461 cgcactgacc tttatccctg ccctggcgct tggtccggtg gcggaatatc tctcctgatg
     2521 atattgagtg agcactgaat atgagtcgta aacaactggc gctattcgaa ccaacacttg
     2581 tcgttcaggc gctgaaagaa gcggtgaaaa aattaaaccc gcaggcgcaa tggcgcaatc
     2641 cggtgatgtt tatcgtctgg atcggcagtc tgctgaccac ctgtattagc atcgcgatgg
     2701 caagcggcgc gatgcctggc aatgcgctgt ttagcgtggc cattagcggc tggctgtggg
     2761 tcaccgtact gttcgctaat ttcgccgagg cgctggcaga aggccgcagt aaagcgcagg
     2821 ccaacagcct gaaaggggtg aaaaaaacgg cctttgcccg caagctgcgt gagccgaaat
     2881 atggcgctgc ggcggacaaa gttcccgccg atcaactgcg taaaggcgat atcgtactgg
     2941 tagaagcagg cgatattatc ccctgcgatg gtgaagttat tgaaggcggc gcatcggtcg
     3001 atgaaagcgc catcaccggg gaatcggcac cggtgatccg tgaatccggc ggcgattttg
     3061 cctccgtcac cggcggcacg cgtattcttt ctgactggct ggtgattgag tgtagcgtta
     3121 accccggcga gacatttctg gatcggatga tcgcgatggt ggaaggcgca cagcgacgca
     3181 aaacgccgaa cgagattgcc ctgaccatcc tgctgattgc cctgactatc gtctttttac
     3241 tggcaaccgc cacgctgtgg ccgttttccg cgtggggcgg taatgcagtc agcgtaacgg
     3301 tactggtggc gctgctggtc tgtctgatcc caaccactat tggcggcctg ttgtcagcga
     3361 tcggcgtcgc cgggatgagt cggatgctgg gcgcgaatgt ggttgccacc agcggacgtg
     3421 cagttgaagc ggcaggtgac gttgacgttc tgctgctgga taaaaccggc accatcacac
     3481 tcggtaaccg tcaggcatcg gagtttatcc ctgcgcaggg cgtggatgaa aaaacgctgg
     3541 ctgacgccgc acaactggct tcgctggctg atgaaacgcc ggaaggccgc agtattgtga
     3601 tcctcgccaa gcagcgtttt aacctgcgcg agcgcgatgt gcagtcgctc catgccacct
     3661 ttgtaccgtt tactgcgcaa agccggatga gcgggatcaa tatcgacaac cgcatgatcc
     3721 gtaaaggttc tgtcgatgcc attcgccgcc atgttgaggc taacggtggt cacttccctg
     3781 ccgatgttga tcaaaaagtc gatcaggttg cgcgtcaggg ggccacaccg ctggtagtag
     3841 tagaaggttc tcgtgtgctg ggcgttattg cgttgaaaga tatcgtcaaa ggcggcatta
     3901 aagaacgctt cgcccaactg cgcaaaatgg gtattaaaac ggtgatgatt accggcgata
     3961 accgtctgac tgccgccgcg atagctgcgg aagcgggcgt cgatgatttt ctcgccgaag
     4021 cgacaccgga ggccaagctg gcattgatcc gtcagtatca ggcggaaggc cgtttggtag
     4081 caatgaccgg cgacggcacc aacgatgctc cggcgctggc gcaggcagat gtcgcggtgg
     4141 cgatgaactc cggtactcag gcggcgaaag aagcgggcaa tatggtggat ctcgactcta
     4201 acccgaccaa gttgatcgag gtggtacata ttggcaaaca gatgctgatg acccgtggct
     4261 cgctgaccac cttcagcatt gccaacgatg tggcgaaata cttcgccatt attccggcgg
     4321 cattcgcggt aacgtatcca cagttaaatg cgctgaacat catgcgcctg cattcgcccg
     4381 actccgccat tctcagtgcg gtcattttca acgccttgat tatcgtcttt ttgattcccc
     4441 tggcgttaaa aggcgtgagt tataaaccgc ttaccgcttc tgccatgttg cgccgtaact
     4501 tatggattta cggtctgggt gggctgctgg tgccgtttat tggtatcaaa gtcattgatt
     4561 tactactgac cgtttgcggt ctggtgtgag gtttaccatg cgtggattac gtccggcatt
     4621 atcgacattt atctttctgt tattgattac tggcggtgtt tacccgctgc tgaccaccgc
     4681 gctggggcaa tggtggtttc cctggcaggc caacggttcg ttgatccgtg aaggtgatac
     4741 ggtgcggggt tcggcattaa tcgggcagaa ttttaccgac aacggctatt ttcatggtcg
     4801 cccgtcggca acggcagaaa tgccttataa tccacaggct tctggcggga gcaatctggc
     4861 ggtcagtaac cctgagctgg ataaactaat agccgcacgc gttgctgcat tacgggccgc
     4921 taacccgaat gccagcacga gcgttccggt tgaactggtg acggcgtcgg caagcgggct
     4981 ggacaataac atcaccccgc aagcggcggc ctggcagatc ccacgcgtag cgaaagcgcg
     5041 taatctcagc gttgaacagc tcacgcaact gatcgcaaaa tacagccaac aaccactggt
     5101 gaaatatatc ggccagccgg tcgtcaacat tgtcgaactc aatctggcac tggataaact
     5161 tgatgaataa cgaaccctta cgtcccgacc ccgatcgtct gctggaacaa actgccgcgc
     5221 cgcatcgggg gaagctgaaa gttttcttcg gtgcctgcgc aggcgtcggg aagacctggg
     5281 cgatgctggc agaagcccag cgactgcggg cgcaagggct ggatattgtg gttggcgtgg
     5341 tagaaaccca cgggcggaaa gataccgccg ccatgctgga agggctggct gttctgccgc
     5401 caaaacgcct ggcgtaccgt gggcggcaca tcagcgagtt tgatctcgat gccgccctcg
     5461 cccgccgtcc ggcgctgatc ttaatggacg aactggcgca cagtaatgcg ccaggttctc
     5521 gtcatcctaa acgctggcag gatatcgaag aactgctgga agctggcatt gatgttttca
     5581 caaccgtcaa cgttcagcat ctggaaagtc tgaatgatgt ggtcagcggc gtcaccggaa
     5641 ttcaggtacg ggaaaccgtg cccgatcctt ttttcgatgc cgccgacgac gtggtgctgg
     5701 tggacttgcc cccggacgat ctgcgccagc ggctgaaaga aggcaaagtc tatattgccg
     5761 ggcaggcgga gcgcgccatt gaacattttt tccgcaaagg caatctgatc gccctgcgca
     5821 aactggcact gcgccgtact gccgatcgcg ttgatgagca aatgcgcgcc tggcgggggc
     5881 atcctggcga agagaaagtc tggcacacgc gcgacgcgat ccttttatgc atcggccata
     5941 acaccggcag cgaaaaactg gtccgcgcgg cggcgcggct ggcgtcacgg ctggggagcg
     6001 tctggcacgc ggtgtatgtt gaaacccctg ccctgcatcg cttaccggag aaaaaacgtc
     6061 gggcaattct cagcgcctta cgtctggcgc aggagctggg cgcggagacg gcaacacttt
     6121 ctgatccagc ggaagagaaa gcggtagtgc gttatgcccg tgaacataat ctcggcaaga
     6181 ttattctcgg tcgcccagcc tcgcgccgct ggtggcgtcg ggaaacgttt gctgaccgac
     6241 tggcgcgcat cgcccccgat ctcgatcagg tgctggtcgc gcttgatgaa ccacccgccc
     6301 gcacgattaa caacgcgccg gatagccgct cttttaaaga caagtggcgg gtacagattc
     6361 agggatgcgt ggttgccgcc gcgttatgcg ccgttatcac cttaattgcc atgcagtggc
     6421 tgatggcgtt tgatgccgcc aacctggtga tgctgtatct acttggcgtg gtggtggtgg
     6481 cgctatttta tggacgctgg ccttcggtgg ttgctaccgt cattaatgtg gtgagtttcg
     6541 atctcttttt tatcgcccca cgcggcacgc tcgccgtctc tgatgtgcaa tatctgctga
     6601 ccttcgcggt gatgttaacc gtcgggctgg tgatcgggaa ccttactgcg ggtgtgcgtt
     6661 atcaggcgcg ggtagcccgt taccgcgagc aacgcacacg gcacttatat gaaatgtcga
     6721 aagctctggc ggtgggccgc agtccgcagg acatcgccgc caccagcgaa caatttattg
     6781 cctccacgtt tcatgcccgc agtcaggtgt tgttgcccga tgacaacggt aaattgcagc
     6841 cgttaacaca tccgcaagga atgacgccgt gggacgatgc catcgcgcag tggagttttg
     6901 ataaaggcct gcctgcgggt gcgggcaccg acacgttacc cggcgtgccg taccagattt
     6961 tgccgttaaa aagcggtgag aaaacctacg ggctggtggt ggtggaaccg gggaatctgc
     7021 gtcagttgat gatcccggaa cagcagcgcc tgctggagac gtttacgctg ttagtcgcca
     7081 atgcgcttga gcggctgacg ctgaccgcca gcgaagaaca ggcgcggatg gcaagcgaac
     7141 gtgaacagat ccgcaacgcc ctgctggcgg cgctttcgca tgatttacgc actccgctta
     7201 cggtgctgtt tggtcaggca gaaatcttaa cgctcgatct ggcaagcgaa ggatcacccc
     7261 acgcccgcca ggccagcgag attcgtcagc atgtgctgaa cactacccga ctggtgaata
     7321 atctactgga tatggcgcga attcagtccg gcggctttaa tttgaagaaa gagtggttaa
     7381 cgctggaaga agtagtcggc agcgcgctgc aaatgctgga accgggttta tcgtcgccca
     7441 tcaatctttc tctgccagaa ccgctgacct taatccacgt tgacgggcca ctctttgaac
     7501 gggtgctgat taatctgctg gagaacgcgg tgaaatatgc gggtgcgcag gccgaaattg
     7561 gtatcgatgc ccacgttgag ggcgaaaatc tacaactgga tgtctgggat aacggccccg
     7621 gtcttccgcc aggccaggag cagacgatat ttgataagtt tgctcgcggg aataaagagt
     7681 cggcagtacc gggggtaggg cttggactgg caatttgtcg ggcgatagtg gatgtacacg
     7741 ggggcactat taccgcgttc aaccggccgg aaggtggtgc ctgttttcgt gttacacttc
     7801 cccagcaaac tgcccctgaa cttgaagaat ttcatgagga tatgtgacaa acgttctgat
     7861 tgttgaagat gaacaggcta ttcgtcgctt tctgcgcacg gcgctggagg gcgacgggat
     7921 gcgcgtctat gaggccgaaa cgctgcaacg cggcttgctg gaagcggcta cccgtaagcc
     7981 agatttgatt attctcgatc tcggcctgcc cgatggtgat gggattgagt ttatccgcga
     8041 cctgcgccag tggagcgcgg tgccggtgat tgtgctttcc gcacgcagcg aagagagcga
     8101 caaaatcgcc gcgctggatg ccggagcgga tgattatcta agtaagccgt ttggcattgg
     8161 cgaattgcag gcccgtctgc gcgtcgcttt acgccgccac tccgccaccg ccgcgcccga
     8221 tccgctggtg aaattttccg atgttaccgt cgatttagcc gcccgcgtga ttcaccgggg
     8281 tgaggaagag gtgcatctca ccccgattga gtttcgcctg ctggcagtgc tgctcaacaa
     8341 cgccggaaaa gtgctcaccc agcgccaact ccttaaccag gtgtgggggc caaacgcggt
     8401 cgaacacagt cattatttgc gtatttatat gggacatctg cgacaaaaac tggaacagga
     8461 tcccgctcgc cccagccatt tcattactga aaccggtatt ggttatcggt ttatgctttg
     8521 aatttttat
//
LOCUS       DBFIFI010000185         8509 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.185, whole genome shotgun sequence.
ACCESSION   DBFIFI010000185 DBFIFI010000000
VERSION     DBFIFI010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8509
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.185"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(33..314)
                     /locus_tag="N7B39_003886"
     CDS             complement(33..314)
                     /locus_tag="N7B39_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2574 family protein"
                     /protein_id="HGU2423782.1"
                     /translation="MNKYWLSGAVFLAYGLASPAFSSDTTTLTINGRISSPTCSMDVV
                     NNHLQQRCGQLLQRVETNYRASSTAKGVTTEVVAVGNNDKRKIVLNRYD"
     gene            complement(576..1685)
                     /gene="apbC"
                     /locus_tag="N7B39_003887"
     CDS             complement(576..1685)
                     /gene="apbC"
                     /locus_tag="N7B39_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HGU2423783.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1817..3850
                     /gene="metG"
                     /locus_tag="N7B39_003888"
     CDS             1817..3850
                     /gene="metG"
                     /locus_tag="N7B39_003888"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HGU2423784.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYTRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            3991..7800
                     /locus_tag="N7B39_003889"
                     /pseudo
     CDS             3991..7800
                     /locus_tag="N7B39_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615297.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            7813..>8509
                     /locus_tag="N7B39_003890"
     CDS             7813..>8509
                     /locus_tag="N7B39_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HGU2423785.1"
                     /translation="MKTYIYQDEKSHKFWAVEQQGNELHINWGKVGTNGQSQVKSFAD
                     TAAAEKAALKLIAEKVKKGYVEQVDANAHSHDASAKKVLVTEEKASINRQAATNGLPW
                     LADNDPIILPPNIARHALSHRLWPGEPVKKPTKPEKQNLISRLANRTYHYYKNLTFDY
                     SSCSDEWADAISQAISLIKAQKTEIPARTMAVLTFLEICFCQGWNDETSSELLDEIVS
                     TFGIEYATETVIFW"
BASE COUNT         2266 a         2129 c         2055 g         2059 t
ORIGIN      
        1 tttttaaata tgcttaaaac ggtgttggca aattaatcgt agcgatttaa cacgattttg
       61 cgtttgtcgt tattacccac agctaccact tctgtcgtaa cgccttttgc agtagaagaa
      121 gcacgatagt tggtttcaac tcgttgtaac aactgcccac aacgttgctg aagatgatta
      181 ttaacaacat ccatactgca tgtaggggaa ctaattctgc cattgatggt taatgttgta
      241 gtatcagaag agaacgcggg tgatgctagt ccataagcaa gaaaaacagc tcctgacaac
      301 caatacttat tcattgaacg ttatccctgt agtaaaggtt atgccgggta gattgagtgc
      361 ataacacact atagctgtac atccgctaca cagccacgag ggatgataaa gagacagtgg
      421 ttgcacaatc aatcgccttt gtacagtttc ggactaagat ccactgaata ttcgcagtat
      481 taaataagtg ttcattaatg cggcaaaaac aaatacaccg catcaatata acatctctgc
      541 ggtgtatgaa gaataaccag aatggttaat aggcgttaga ccgcgcggaa ggaaatctcg
      601 cctggaatga cttcaccctg ccagtagagc tgagctgcaa cgcggtcagc cagctggcgg
      661 tagatagcgg taaattcgct ctccggacgg ctgataaccg ttggcgtgcc tttatcgaga
      721 tcctcacgta aggagatgtg taacggcatc tgccccagca gctgtgtatg gtatttttca
      781 gccagtttct ctgcaccacc ggtaccgaaa atcggttcat ggtgaccaca gttactgcaa
      841 atatgcacac tcatgttctc gacaataccg agcaccggaa cctcgacttt ctcgaacatc
      901 acaatgcctt ttttcgcatc gatcaacgcg atgtcctgcg gggtggtgac cacgactgca
      961 ccagttactg gaatgttttg cgccagcgtc aattgaatgt caccggtgcc tggcggcata
     1021 tcaagcacca gatagtcgag atccggccac aaggtttcct gcaacatctg cattaacgcc
     1081 ttgctcgcca tcggtccacg ccacaccatc gcattgtcgt cagtgaccag ataaccaatt
     1141 gaattggttg ccaggccatg agacataatc ggtgccatat gggtaccgtc cggcgaagtt
     1201 ggacgctgat tttctgcgcc cagcatggtg ggaattgatg ggccgtagat atcggcgtcc
     1261 agaatgccga ctttcgcccc ttccgccgcc agtgccagcg ccaggtttac cgccgtggag
     1321 gatttaccca cgccgccttt accggaactg accgcaatga tgttcttcac gccattaata
     1381 cccggctgat tcttcacgcg ttttaaggtg gcgatattgt gcgacagttt ccagtcgata
     1441 gctttcgccc cggtgatacg cagcagttcg gcgctacatt gctcttttaa ctcttcaaac
     1501 gcgctatgcc agacgaatgg cataaccagt tccacatgca acgtgtcgtc catccaggcg
     1561 acatggtgca acgctttcag cgtggtgagg ttatgcttca gggttgggtg ctgaaaattg
     1621 gccagtgtcc cggcgaccat tgcgcgcaag gcttctggtg atttggcctg ggattgttcg
     1681 ttcatcccga ctcctttttt gtatagataa accatcagct gatagtttac ctgaagaata
     1741 tagagaagta cttacttaac attttcccat ttggtactat ctaacccctt ttcactatta
     1801 agaagtaatg cctactatga ctcaagtcgc gaagaaaatt ctggtgacgt gcgcactgcc
     1861 gtacgctaac ggctcaatcc acctcggcca tatgctggag cacatccagg ctgatgtctg
     1921 ggtccgttac cagcgaatgc gcggccacga ggtcaacttc atctgcgccg acgatgccca
     1981 cggtacaccg atcatgctga aagctcagca gcttggtatc accccggagc agatgattgg
     2041 cgaaatgagt caggagcatc agactgattt cgcaggcttt aacatcagct atgacaacta
     2101 tcactcgacg cacagcgaag agaaccgcca gttgtctgag cttatctata ctcgcttgaa
     2161 agaaaacggt tttattaaaa accgcaccat ctctcagctg tacgatccgg aaaaaggcat
     2221 gttcctgccg gaccgttttg tgaaaggcac ctgcccgaaa tgtaaatccc cggatcaata
     2281 cggcgataac tgcgaagtct gcggcgcgac ctacagcccg actgaactga tcgagccgaa
     2341 atcggtggtt tctggcgcta ccccggtaat gcgtgattct gaacacttct tctttgatct
     2401 gccctctttc agcgaaatgt tgcaggcatg gacccgcagc ggtgcgttgc aggagcaggt
     2461 ggcaaataaa atgcaggagt ggtttgaatc cggtctgcaa cagtgggata tctcccgcga
     2521 cgcgccgtac ttcggttttg aaattccgaa cgcgccgggt aaatatttct acgtctggct
     2581 ggatgcgccg attggctata tgggttcttt caagaatctg tgcgacaagc gcggcgacag
     2641 cgtaagcttc gatgaatact ggaagaaaga ctccaccgcc gagctgtacc acttcatcgg
     2701 taaagatatt gtttacttcc acagtctgtt ctggccagca atgctggaag gcagcaactt
     2761 ccgcaagccg accaacctgt ttgttcatgg ctatgtgacg gtgaacggcg cgaagatgtc
     2821 taagtctcgc ggtaccttta ttaaagccag cacctggctg aatcattttg acgccgacag
     2881 cctgcgttac tactacactg cgaaactctc ttcgcgcatt gatgatatcg atctcaacct
     2941 ggaagatttc gttcagcgcg tgaatgccga tatcgttaac aaagtggtta acctggcctc
     3001 tcgtaatgcg ggctttatca acaagcgttt tgacggcgtg ctggcaagcg aactggctga
     3061 cccgcagttg tacaaaacct tcactgatgc cgctgaagtg attggtgaag cgtgggaaag
     3121 ccgcgaattt ggtaaagcag tgcgcgaaat catggcgctg gctgatctgg ctaaccgcta
     3181 tgtcgatgaa caggctccgt gggtggtggc aaaacaggaa ggccgcgatg ctgacctgca
     3241 ggcgatttgc tcaatgggca tcaacctgtt ccgcgtgctg atgacttacc tgaagccggt
     3301 actgccgaaa ctgaccgagc gcgcagaagc atttctcaat acagaactga cctgggatgg
     3361 tatccagcaa ccgctgctgg gccacaaagt gaatccgttc aaggcgctgt ataaccgcat
     3421 cgatatgaag caggttgaag cactggtgga agcgtctaaa gaagaagtga aagcagctgc
     3481 cgcgccagtt actggcccac tggcagatga tccgattcag gaaaccatca cctttgacga
     3541 cttcgccaaa gttgacctgc gcgtggcgct gattgaaaac gcagagtttg ttgaaggttc
     3601 tgacaaactg ctacgcctga cgctggatct cggcggtgaa aaacgcaatg tcttctccgg
     3661 cattcgttct gcttacccag atccgcaggc actgattggt cgtcacacca ttatggtggc
     3721 taacctggca ccacgtaaaa tgcgcttcgg tatctctgaa ggcatggtga tggctgccgg
     3781 tcctggcggg aaagatattt tcctgctaag cccggatgcc ggtgctaaac cgggtcatca
     3841 ggtgaaataa tcccccttca aggcgctgca tcgacagcgc cttttcttta taaattccta
     3901 aagttgtttt cttacgattt tgtctctctc taacccgtat aaatactggt agcatctgca
     3961 ttcaactgga taaaattaca gggatgcaga atgagacact ttatctatca ggacgaaaaa
     4021 tcacataaat tctgggcggt tgagcagcag ggaaacgagc tgcatatcaa ctggggcaaa
     4081 gtcggcacta acgggcaaag ccagataaaa agttttgcgg atgttgcagc agcggaaaaa
     4141 gcgaaactga agcttatcgc ggagaaaacg aaaaaaggct atgtggaaaa tgcttcagca
     4201 aacgtgcata tcccccccat caccaaagcc actcctgaag ttgaaacttc ccctgagagt
     4261 aaaaaccaac gcccgtggct ggctgatgat gcagttattc cggtaactga cgatattaac
     4321 cgatttgctt ttcctcatcg ctctcgcccc agagagatca attatctgcg caaagacggt
     4381 gagatatgga agcgtattgc agataacacc agggcatatg atcccgacaa caattaccgc
     4441 tcttatcctg aaaactggca gcaggctttt gctgagttac aaaggcgaat tctggataat
     4501 caacagacag ggagtgcgca atctgatgca gcattgctct ggagtttttg gaactcttac
     4561 tctgcagatg aactggtcga tgacttagtc attcgctgtg gtctggaaag cgccgttgaa
     4621 atcgtccttt ttgcccttca actcaaatat aaaccagtaa aaggcgcagt aaccaccatc
     4681 attccaccta atcacgaagc agaatcgcta cctagttagc atcaacgtct atgtcatcat
     4741 ctttcactcg cttcagaaga tgagtggcaa cgctgtgtag ataaagtact caccgccatt
     4801 ccgacgctat cccccgcacg gcaacctttt gcggcgttac tgctccctga acgcccggat
     4861 atcgcccatg ctatagatct acgctatgca gaccaaaacg tcccggcgat gacctggtta
     4921 agactggtga taaccgatga tactgctctg agtacagtgg aaaaatacga cttcccaccg
     4981 ttgtatcgtg attttcgtaa ttttcgcgct tatctggcaa tgttgttggc aaataatggg
     5041 gtgcgcggtg taagccggat acttcttgag tttacggaag accattctga caatcccact
     5101 tatctcttcg aacgcatcag cgagactgaa aatcttgtca aatggctatg gaaaacgaat
     5161 cacccggatg cgattcaaat tctgatcctc ggtgtaattg gtaagaaaaa gcacctggaa
     5221 tacttaagca aggccagtca aaagcatccc gctgcggcta ttgcggctta tgctactttg
     5281 ctggcaatac atgaagataa agagtggcgt aaagcgcttg tcaaactgat taccgccacg
     5341 ccagagttag tatgcgacgt cattccctgg gttaatgcta aagcggcagg catcttatct
     5401 gaatgtcgtc cgcagtcagt cgctgaagaa tgtgaatatg ccactgtgga tatgttgccg
     5461 gagctgcttg tttcgccgcc ctggatgacc aaagagaaaa agaaaaatac gcctgtgttt
     5521 gatcttccag tgcttccagt tccttctgtt tctgatgtca ctccagagat taccaaaaaa
     5581 ctgacccgta cttaccttgt cacccacttc cagcaaatag cccaacagca ggcgacaaaa
     5641 caaacattgt ttaccgacct gccgccgatc aaaaaagcaa gctgggagaa acacttaata
     5701 ccgcttacac cggagcaaca aatactgtgg catcttggtt ttgaaaaatg gcgggagagc
     5761 ggagaaaaaa tatacgaaaa aattccggcg ccacaaagcg ccgttgatgc cctgctacgc
     5821 ttcgattttc cagcactcaa tgctgaattt gtgcattatc ataacaatgc atacaaaagc
     5881 tggaatctca ttgccctctg ttacctgcct ggtcaacagg ccatttcttt tcttaaccag
     5941 atagtcaaag aagataacta ctctggtgag ggaaatattc tggcaatttt tggtagcgcc
     6001 gccatacctg catttatggc ctgcctgcaa cgtgatccac gacgtctgtg cttcttcccc
     6061 tttttcctcg gtgtcagtga actggcctta ccgatggcgc aacaattaca gaaaaaaatg
     6121 tcttatgaag atgcgcgaaa ctggctaact gattatccac gccatgccgc cgccgggctg
     6181 cttcctgtgg ccctgggcaa aaaaggaaaa gatcgtgatt gtgcccgcca ggcgcttcgt
     6241 ctgctggtga atttaaacca gcgagaaacg attgaggaaa tcgcgcaagg atataatcaa
     6301 cctgatgttt tagctgcgct ggcaacattg ttcgatagcg acccactgga agaatatccc
     6361 gctaaaatcg ccccactgcc cggcttttat cagttcacct tgtggcgcag accacggctt
     6421 aaaagtaaca acctgcctct gtcagatgac gctatgcgcc acctcggcac tatgctgagc
     6481 tttcctcgcg acattaccgc ctacgctggg ctggatatca ttagagaaat cttcacccgc
     6541 gaatcactgg ctgaatttgg ctgggatctg tatcccgcct ggacagaagc tggcgcaccc
     6601 gcaaaagaaa actgggcatt tacttcgctt gggattttag gcaatgacga caccgcgcgt
     6661 aaattaaccc cgcttatccg cgcctggcct ggggaatccc agcataaacg ggcggtgtcc
     6721 gggctggatg tattagctga tattggcagc gatgttgcgc taatgctgct taatggcatc
     6781 gcgcgaaaaa ttaaattcaa agcattacag gaacatgccc gcgagaaaat caacatagtt
     6841 gctgaaaacc gtgggctgac tatggctgaa ctggaagacc gcctggctcc agatttaggg
     6901 cttgatagca gtggctcgct gatactggat ttcggccccc gcaaattcac cgttggtttt
     6961 gacgaaacct taaaacctgt agtgtgcgat gcaaacggca aagtcctgaa agatttacct
     7021 aagccaaacc agagcgatga aaaaactcag gcaactgacg cggttaatct cttcaaacag
     7081 ttgaaaaaag atgtacgcgc catagccagc cagcagattg atcgtctgga acaggctatg
     7141 tgccagcgca gacgctggac ggcagagcag ttccgcctgt ttctggtgga gcatccgctg
     7201 gtacgtcact taacccgacg gctgttatgg ggggtttata acgatgaaaa cgcccttatc
     7261 acctgttttc gcgtggcaga agacagcact tacagcgatg cgcaggatga gttattcacg
     7321 ctgccagcag gaaacatcgg tattccgcat gtgctggaaa tatcccctga atccgccgct
     7381 gcattcaggc aaatttacgc tgattacgaa ctgcttcccc ctttccaaca gctcgagcga
     7441 ggtagctatc accttgctga taatgaacgt aatactcacg aactgacacg ctggcaagga
     7501 cggctttgcc aggccggacg cattgtcggg ctggaacgca gaggctggca acgtctggaa
     7561 gaaagcggca gcgtttatgc gatgcgcaaa acaacccctc atggcgatct cgaacttgaa
     7621 acggaacctt tttcattaat ttatggtgaa acggggtatg gcgaccagca cccggttgaa
     7681 agcgtaaaaa tcacctcgcc agatgatcgc tacggtaaac aatcctcact cactttctcc
     7741 atgctcgacg acatcaccgc cagcgagctg attaacgaca ttgaatcact gtttgattaa
     7801 aaggatttaa caatgaaaac ctatatttat caggacgaaa aatcacataa attctgggcg
     7861 gtggagcagc agggaaacga gctgcatatt aactggggta aagtcggtac caacgggcaa
     7921 agtcaggtaa aaagctttgc agatactgcg gcggcggaaa aagcagcact gaagctgatt
     7981 gcggagaagg tgaagaaggg gtatgtggag caagtagacg ctaacgctca ctcacatgat
     8041 gcctcagcaa agaaagtcct ggtcacagag gaaaaagcct caataaatcg ccaggcagca
     8101 actaatggtt taccctggct ggcagacaac gatcccatta ttcttccccc gaatattgcc
     8161 cgccatgccc tcagccaccg cctttggccg ggagagccag taaaaaaacc aaccaaaccc
     8221 gagaaacaaa atttaatttc ccgattggca aatcgtactt atcattatta taaaaatctg
     8281 actttcgatt acagctcatg ctctgatgaa tgggctgatg cgattagcca ggcaatcagc
     8341 ttaattaagg cccaaaagac tgagataccc gcgcgtacta tggcggtact tacttttctg
     8401 gaaatatgtt tttgtcaggg ctggaatgat gaaacctcat ccgagttgct ggatgaaatt
     8461 gtaagcacct tcggtatcga atacgcaact gaaactgtca tcttctggt
//
LOCUS       DBFIFI010000186         8390 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.186, whole genome shotgun sequence.
ACCESSION   DBFIFI010000186 DBFIFI010000000
VERSION     DBFIFI010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.186"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            88..1866
                     /locus_tag="N7B39_003891"
     CDS             88..1866
                     /locus_tag="N7B39_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HGU2423786.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLYRVAG
                     TGAYGKFLGRQLSGNSRLLCHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVTEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(1906..2385)
                     /gene="xdhC"
                     /locus_tag="N7B39_003892"
     CDS             complement(1906..2385)
                     /gene="xdhC"
                     /locus_tag="N7B39_003892"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HGU2423787.1"
                     /translation="MNHSETITIECTINGMPFQLHAVPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(2382..3260)
                     /gene="xdhB"
                     /locus_tag="N7B39_003893"
     CDS             complement(2382..3260)
                     /gene="xdhB"
                     /locus_tag="N7B39_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HGU2423788.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDSITQRHLPALCAAASSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHVGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(3271..5568)
                     /gene="xdhA"
                     /locus_tag="N7B39_003894"
     CDS             complement(3271..5568)
                     /gene="xdhA"
                     /locus_tag="N7B39_003894"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HGU2423789.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWEELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYVSHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNASREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            5984..6739
                     /gene="actS"
                     /locus_tag="N7B39_003895"
     CDS             5984..6739
                     /gene="actS"
                     /locus_tag="N7B39_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HGU2423790.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            6818..6891
                     /locus_tag="N7B39_003896"
     tRNA            6818..6891
                     /locus_tag="N7B39_003896"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6850..6852,aa:Gly,seq:ccc)
     gene            7125..8390
                     /locus_tag="N7B39_003897"
                     /pseudo
     CDS             7125..8390
                     /locus_tag="N7B39_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
BASE COUNT         2213 a         1947 c         2080 g         2150 t
ORIGIN      
        1 aatttcgcct gagttaatat gatcatgatc tgtgaaccat caacgtcttc aatgatcgac
       61 ttttaagtaa gtctggaagg taaagctatg gagcttgcta ctacgcagtc agtattgatg
      121 caaattcaac cgacaattca gcgttttgcc agaatgcttg ccagcgtttt gcagcttgag
      181 gttgagatcg ttgatgaaaa cttgtatcgc gtcgccggaa cgggcgcgta tgggaagttt
      241 cttggtcgcc agttgagcgg caactcacgc ctgctctgcc acgtcctgga aacgaaaact
      301 gaaaaagttg tgacacagtc tcgcttcgat cccctttgcg aaggttgcga tagtaaagaa
      361 aattgccgcg aaaaagcatt tctgggtacg cctgtcattt tacaggatcg ttgtgttggg
      421 gtgataagtt tgattgccgt tacccacgag caacaagagc atatcagtga taatttacgc
      481 gaattttctg attatgttcg ccatatatcc accatttttg tttcgaaact tctggaggat
      541 caggggccag gagataacat cagtaaaata ttcgcgacca tgatcgataa tatggatcag
      601 ggcgtattag ttgttgatga tgaaagtcgg gttcagtttg ttaatcagac tgccttaaaa
      661 acacttggtg ttgtgcaaaa taatattatt gggaaaccta tccgtttcag accattaaca
      721 tttgagagta attttaccca tggacatatg cagcatattg tttcgtggga cgataaaagt
      781 gaattaatca ttggtcaatt gcataacatt cagggccgac aattattttt aatggcgttt
      841 caccaatcgc ataccagttt ttctgtagca aatgcacctg atgaaccgca tattgaacaa
      901 ttggttggcg agtgccgtgt tatgcggcaa ttaaaacgac tcattagccg tattgcaccc
      961 agcccatcca gcgttatggt ggttggtgaa agcggcacgg gtaaagaagt tgtcgcccgt
     1021 gcaatccata agttgagcgg aagacggaat aaacccttta ttgctatcaa ctgtgccgcg
     1081 attccggagc agcttctgga gagcgaactg ttcggttatg ttaaaggcgc atttactggc
     1141 gcttctgcca acggtaaaac agggttgatt caggcggcga atacgggcac gctgtttctc
     1201 gatgaaatag gtgatatgcc attaatgttg caggctaaat tactgcgcgc tattgaggca
     1261 cgtgaaattc tgccgattgg tgccagtagc ccaatacaag tcgacattcg catcatttct
     1321 gcaactaacc agaatttggc ccagttcatt gccgaaggta aattccgcga agatctcttc
     1381 taccgactta atgttatccc gataactctg ccaccgctgc gtgaacgtca ggaagatatt
     1441 gaactactgg tgcattactt tttacatctg catacccgtc gtctgggatc ggtttatcct
     1501 ggcattgctc ccgatgtcgt cgaaatattg cgtaagcatc gttggcccgg aaacctgcgc
     1561 gagttaagca atttgatgga atatctggtt aacgttgttc cttcaggtga agttatcgac
     1621 agcacgctat tgccgccaaa tctgctgaat aatggcacaa cggagcaaag tgatgtaaca
     1681 gaggttactg aggcacacct gtcactcgat gatgcgggcg gcacggcgct ggaggagatg
     1741 gaaaagcaaa tgatccgcga ggcgctttca cgtcataaca gcaagaagca agttgctgat
     1801 gaactgggca tcggcattgc tacgctctat cgcaagatta agaaatatga gttgttaaac
     1861 acataacatc agtgaatccg gcctgaattc aggccggata tccttttact tcgttttctc
     1921 gcaatccaga actgtattta caatcatctg ataccccgtg cagcgacaaa gatttcccgc
     1981 cagtccgcga cgaatttccg taatggttaa tggcttctcg cgtggtttcg ccagcattgc
     2041 cgtggtagcc ataatcaggc caggcgtaca aaacccgcac tgcactgcgc cggatttcgc
     2101 ataagcctgc tgaacatgag aaagttttcc gcctttcgct tcaccttcca gcgtgcggat
     2161 ctcttttcct tcagcccagg cggcaaggta taagcaactg tctattgctg tgccgtcgac
     2221 cagcaccgta caggcaccac attcacccac gcagcaccct tgtttgacac ttagcagccc
     2281 ttgttcgcgg agtaattccg agagcggcgt gcctggtacg gcatgaagct gaaaaggcat
     2341 cccgttaatg gtgcattcga tggtaattgt ttcgctgtga ttcattgcaa ttttcccccc
     2401 gccgcggcga cggcttcgct aatcactttt ttggtcatcg tctggatgag atgcagacga
     2461 aactctttac tggcccgcca tgaagaacgc ggggcgacat cttgcaggac agattcgctg
     2521 atagcttcca gcgtttgcag gtttaatggc gcattttgtg cagtctgttc ggcatgttgg
     2581 cagcgaatcg gcgttggcgc ggcaacacca aatgccaggc gtaattcgct gaaattgccg
     2641 ttatccagtc ggcaatgtgc ggcacagcca atcgttgaaa tatccattgc gtcgcgcatg
     2701 gcatatttaa aatgcgcgct gcccacgtgt tctttcggct ggggcggaaa atgaaaggcg
     2761 acaaggattt cgtcatgctc aagagacact ttgcccggcc cggtgtgaaa gccattaatc
     2821 gggacgaaac gaacaccgcg tggggagtgg atctccagtt tcgcgtcata aatgagcgtt
     2881 ggcgtggcag aatctgcgct ggtggcaccg ttgcaaatat ttccaccgta ggtagcgacg
     2941 ttacggatct gcggtccagc aatggacgag gccgcagcac ataacgccgg gagatgacgt
     3001 tgagttatag aatcttctat tagctgggta aatgtcgttg cagagccgat acgtagcgag
     3061 ccatcttccg ccagcgtaat tccccgcagc tccgccagat tatgaatatc aacaatatgg
     3121 cgataacggt cattgtgatg gtggagctga atcagtacgt cagtgccacc ggcgagcagt
     3181 ttggcctgcg ggttgtcagc cagcaggttg atggcatcgg caagggttgc tgcgcgatgg
     3241 taagaagcaa aatcaaacat gatgttatcc tcaaatcaat cctgccagat ggaactcttc
     3301 atataaccgt tttggcgtca gcggcagtgt attgattgca acaccggtag ccatcttcac
     3361 cgcgttacga atagcagcgg caacaggaat tattggtggc tcacccagtg acttatgtcc
     3421 gtatgcggat tgcggctcat tgatttcgac gaacgcgctt tccagttgtg gcagatccgg
     3481 catggtcggc attttgtaat ccagcagatt ggggttacgg accacgccgc ttttcgcatc
     3541 gatgatcatc tcttcaaata gcgcccagcc aatgcccatt cccattccgc cgtgtacctg
     3601 accttctgcc agtagcggat taagaatatg gcccgaatca tgaacgttga ggatgcggtt
     3661 gatggtgact ttgcacagcg caatatcgac cgtcagatca acaaaggtac agccgaacgc
     3721 cggtgggtta gtggtggttt tgatggagct ttcagcagag agctgcccgc cgcgttcagg
     3781 gtggtagaaa gcgtccatcg ccaaatcttt taacgacatt aacggctctt ccggtcgttc
     3841 aaccagcacg atatggcctt ttatcagggt cagattcatc gctgactgat gtagcatgac
     3901 tgcggcgtga gcgatgattt tctcttttaa taacagtgcc gcactgcgca gcgcaggcgc
     3961 ggcaacatag ctctggcgtg aggcaaatgc gccgggatcg aacggcgtaa cgtcggtatc
     4021 ttgtgttgaa ataacgcgaa cgtcgctgac cggaaccccc acggtttctg ccaccatttg
     4081 cgagaagacg gtgtcggcac cctgaccgat ttccgtcgcg ccgctttgca cgttgatggt
     4141 tccatcctga ttcatcagaa ggcgcgcgcc tgctatttct acgccgacag gccaggtgtt
     4201 agaggtgtag ctaaaacagg cgacgccaac gccgcggcgt aaattgcctt gctggttctg
     4261 gcactctgca cggcgttttt cccattcaaa gattttccgg cctttttcaa gacactccgg
     4321 caaccctgcg ctgtaaatac gtttgcccgt gagcggatta gcatctcctt cgcgtgaggc
     4381 gttgcgtaaa cgaatttcaa caggatcaat acctaacgct gtcgcggcgt catcaagcat
     4441 agactcaacg gcaaatacga cttgtggcgc gccataacca cgcatcgcac cagccgaggg
     4501 gaggttggta tagcaggtct ttgaactgta agcgtaggca caacgaggat aaaggtaagc
     4561 gactttattt ccccctgcag aagcgatgga gtgcccgtga gatacataag cgccggtgtt
     4621 agacagaaca tccagactat aacctttcaa tgttccgtcg cggttcacgc ccatttgccc
     4681 gtcaatagta aaagcgtggc gggtacgggt tgcgaggaaa cactcttcac ggctaaggga
     4741 aactttcacc ggaatgccgc caagcttgct ggtcaggaat gccgccattg gctcttccag
     4801 tacatcctgt ttattaccaa aaccgccacc gacaaatggt ttgatgactc gtacgcatga
     4861 ccagggaata tccagcgcct gaccaaccac gcggcgaaca atgtgcggga tctgggtgct
     4921 ggaaacgatg gtaattcgcg agtcatcctc catccacgcc agcgatgtta cgctttccat
     4981 gtgacaatgt tgaataacgg gggtctgata gtgcccctgt acctggtagt cggcggcatc
     5041 gattgtttgt tggacattgc ccgtcgacat cgtgctttgt ttcagtaaat tgccgccgtt
     5101 atggattggt gcagcgtcct ctgccagcgc cgcttctggc gtggtgataa cgggtaattc
     5161 ttcccactca atgctgacca attgcgccgc tttttctgcc gtgagttcat cgcgggccac
     5221 gacgatggca acggcgtcac catgatgacg aacatggcga gttagcagtg cgcgatcggc
     5281 ggtatcgcgc ttgttttcgt caagtgtcca tgcatgccca gctgtagcga atgggatatc
     5341 aggcacatct tcccaggtaa aaatcgccag tacgcctggt aaacttctgg cttgttcatc
     5401 attaatactt acggcataac catgtgcgat agggctacgt acatatttcg cataacacat
     5461 gcccgccata acataatcgt cagtatatcg tgcccgcccg gtgaccttag caatggcatc
     5521 aacgcgcatg catgattcac ccgtagcggt tgcttcccgc gcttccatag aaatcccctc
     5581 gatttgaaca tttggttctt atggcaaaga tttagtctaa gcaggcaaga tttacgccag
     5641 aaacggcgat aagcgttaag ggaggaagga agtgtgatga tgttaaaaaa acacagatat
     5701 gatacgaaac aggaccctga ataggggtag tgatacgtat caatatgagt ggcgataaaa
     5761 tcaatgtgat aaattaggat tattacgcat caggatggga aatagggtaa aaaagtcagg
     5821 aacttcgata cctctacagt gaatattttg ctgatgatac ttccctttgc caaatgaaat
     5881 taacctcttt ctctattaca tcgtttttcc agaagcaaga gattgggtat caagaggctg
     5941 gctgctatga tatggcgtct tgttttttaa gcgaggaaag attttgagtg cgggacgcct
     6001 gaataaaaaa tctctgggta tcgtgatgtt gttatcggtt ggactgcttt tggcgggctg
     6061 ttcgggtagc aaatcatccg atacaggaac gtattccggc tccgtttaca ccgtgaaacg
     6121 gggggatacg ctatatcgta tttcgcgcac cacgggaacc agcgtaaaag agctggcgcg
     6181 actgaacggc atttcccccc cttacaccat tgaagttggt cagaaactaa aactgggtgg
     6241 ggcgaaaagt agcagtagta cacgtaaatc aaccgccaaa tcaacgacca aaaccgcatc
     6301 ggttacaccg tcatcagcgg taccgaaatc ttcctggccg ccagtagggc aacgttgttg
     6361 gttatggcca acgacaggga aagttatcat gccgtattcg acagcagatg gcggcaataa
     6421 agggattgat atctcagctc cacggggtac acctatttac gccgcgggtg caggaaaggt
     6481 ggtgtatgtg ggcaaccagc tgcgtggcta cggtaatctc atcatgatta aacacagtga
     6541 agattacatt acggcttacg cccataatga cacgatgctg gtaaataatg ggcaaagcgt
     6601 gaaggctggg caaaaaatcg ccaccatggg gagcacggat gcggcatctg ttcgcctgca
     6661 tttccagatt cgttaccgtg caacggcaat tgatccgcta cgttacttgc cgcctcaggg
     6721 cagcaagcca aaatgctgat ggcgaattaa tcagcagtca gcagcgcggc acttgctaag
     6781 aagagcgtaa ggtttataat gccttacgca tctcgaagcg ggcgtagttc aatggtagaa
     6841 cgagagcttc ccaagctcta tacgagggtt cgattccctt cgcccgctcc aatttatctt
     6901 cacctcatca atctgccatt tttgctgtat ctgattttct atattatctt aattcaatcg
     6961 cttcagactt ttcgcaatct ctgtaacact cccccatttt tttgtaaata aaaagcgttt
     7021 acgcaatgca atccttttgt aacaacccag gtaaaatacc ttattttcaa gttgttaaat
     7081 aagtggttat ctacgctttt aacaaacgat tgatggaata ttttatgaaa gggaaatctg
     7141 cattaactct tctgctcgct ggtatatttt cgtgcggcac atgccaggcg acaggggcag
     7201 aagtgacgag tgaatctgta tttaacattc ttaattccac gggggcagca acagataaaa
     7261 gttatttatc gttaaatcca gataagtatc ctaattatcg tcttctgatt cactcagcca
     7321 aattacaaaa tgaaataaag agccactata ccaaagacga aattcaagga ttgctaacat
     7381 taacagaaaa cacacgaaag ttaacgttga cggagaaacc ctggggaacg tttattcttg
     7441 cttctacttt cgaagatgac aaaacagcgg cagaaaccca ctatgatgct gtctggctga
     7501 gggatagttt atggggctat atggcattag tatcagacca gggaaatagt gtagccgcaa
     7561 aaaaggttct gctcaccctg tgggattata tgtctacgcc cgaccagatt aagcggatgc
     7621 aggacatcat tagcaaccca aaacgactgg atggtattcc cgggcaaatg aatgctgtgc
     7681 atatttgaca gcaactcccc cgtgatggca gatgtgcagg aagaaggcaa gccgcagctg
     7741 tggaaccata aacaaaatga tgcgttaggt ctttatcttg atctccttat acaagcaatc
     7801 aatactggta ctattaacgc tgaagattgg caaaaaggtg accgactaaa atcggttgcg
     7861 ctgctgattg cttatctgga taaagccaat ttctatgtta tggaagattc cggagcgtgg
     7921 gaggaagatg cccgattaaa cacatcttcc gttgcactgg tgacctctgg actggaacgt
     7981 ttgtcaaacc tactctcgaa gaaagactct gtttttgtct ctgatttact acgcgaagct
     8041 aaagtcaatg aactggatga aactctttca acaacgaggt tgaaccacct catagataaa
     8101 ggttatgaaa gaataacgtt acagcttgat ttagggggag aatccccagg ttatctggaa
     8161 aaagataaac attacagaga agccgatgcg gctttactta atgttattta tcctgcaaat
     8221 ctttcaaaga taaacaccag aagaaaagag caagttctaa aaatagttaa aaaactagca
     8281 ggtccgtatg gtataaaaag atacgagaag gataattatc agtcggcaaa tttttggttc
     8341 aatgatatta aaaccgatac cgatcaaaat tcgcatgcaa aaagagaaaa
//
LOCUS       DBFIFI010000187         8350 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.187, whole genome shotgun sequence.
ACCESSION   DBFIFI010000187 DBFIFI010000000
VERSION     DBFIFI010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8350
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.187"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            287..1627
                     /gene="dcuB"
                     /locus_tag="N7B39_003898"
     CDS             287..1627
                     /gene="dcuB"
                     /locus_tag="N7B39_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HGU2423791.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            1705..3351
                     /gene="fumB"
                     /locus_tag="N7B39_003899"
     CDS             1705..3351
                     /gene="fumB"
                     /locus_tag="N7B39_003899"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HGU2423792.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSVETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHHPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIINKQCANCTK"
     gene            3474..4103
                     /locus_tag="N7B39_003900"
     CDS             3474..4103
                     /locus_tag="N7B39_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HGU2423793.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(4242..5663)
                     /gene="melB"
                     /locus_tag="N7B39_003901"
     CDS             complement(4242..5663)
                     /gene="melB"
                     /locus_tag="N7B39_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HGU2423794.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIAFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKIPPEPVHADIPVGAVSDVKA"
     gene            complement(5766..7121)
                     /gene="melA"
                     /locus_tag="N7B39_003902"
     CDS             complement(5766..7121)
                     /gene="melA"
                     /locus_tag="N7B39_003902"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HGU2423795.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYAPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYDAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            7404..8312
                     /gene="melR"
                     /locus_tag="N7B39_003903"
     CDS             7404..8312
                     /gene="melR"
                     /locus_tag="N7B39_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HGU2423796.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACIPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
BASE COUNT         2131 a         2210 c         2052 g         1957 t
ORIGIN      
        1 agaccgataa atatcaataa gataacagca aacaaaacat taacatctgc gcagtacaaa
       61 ctttaaaccc atcgccagag agtttttctc tctgaaaaag ccgcttatca cagtgcataa
      121 atttgccgct gctttaatca gccaatattc actgtgaggt atttgctaaa gccggtaacg
      181 accaaacgga tatttagtca ggctctgaaa acagttcata caaaacagaa cgtgactgtg
      241 atctattcag caaaaattta aataggatta tcgcgagggt tcacacatgt tatttactat
      301 ccaacttatc ataatactga tatgtctgtt ttatggtgcc agaaagggtg gtatcgcgct
      361 gggtttatta ggcggtatcg gtctggtcat tctggtcttc gtcttccacc ttcagccagg
      421 taaaccacca gttgatgtca tgctggttat cattgcggtg gtggcggcat cggcgacctt
      481 gcaagcttcg ggcggtcttg atgtcatgct gcaaattgcc gagaagctgc tgcgccgcaa
      541 cccgaaatat gtctcaattg tcgcgccgtt tgtgacctgt acgctgacca ttctttgcgg
      601 tacgggtcat gtggtttaca ccattctgcc gatcatctac gacgtcgcca ttaagaacaa
      661 catccgtccg gaacgtccga tggcggcaag ttctatcggt gcacagatgg ggattatcgc
      721 cagtccggtg tcggttgcgg tcgtgtctct ggttgcaatg ctgggtaatg tcacctttga
      781 tggtcgccat cttgagttcc tcgacctgct ggcaatcacc attccatcga cgttaatcgg
      841 tatcctggcg atcggtatct tcagctggtt ccgcggtaaa gatctggata aagacgaaga
      901 gttccagaaa ttcatctccg taccggaaaa ccgtgagtat gtttacggtg ataccgcgac
      961 gctgctggat aaaaaactgc cgaaaagcaa ctggctggca atgtggattt tcctcggggc
     1021 aatcgctgta gtcgcacttc ttggtgctga ttcggacctg cgtccatcct tcggcggcaa
     1081 accgctgtcg atggtactgg ttattcagat gtttatgctg ctgaccgggg cgctgattat
     1141 tatcctgacc aaaaccaatc ccgcgtctat ctcaaaaaac gaagtcttcc gttccggtat
     1201 gatcgccatc gtggcggtgt acggtatcgc atggatggca gaaaccatgt tcggtgcgca
     1261 tatgtctgaa attcagggcg tactgggtga aatggtgaaa gagtatccgt gggcctatgc
     1321 cattgttctg ctgctggttt ccaagtttgt aaactctcag gctgcggcgc tggcggcgat
     1381 tgttccggtc gcgctagcga tcggcgttga tccggcatac atcgtggctt cagcaccggc
     1441 ttgctacggt tattacatcc tgccgactta tccgagcgat ctggcagcga ttcagtttga
     1501 ccgttccggc accacccaca tcggtcgctt cgtcatcaac cacagcttta ttctgccggg
     1561 gttgattggt gtgagcgtat cgtgcgtctt cggctggatc ttcgccgcga tgtacgggtt
     1621 cttataaatg cactttgcgt gccgcccgtt actacgcggc acgccatttt cgaataacaa
     1681 atacagagtt acaggctgga agctatgtca aacaaaccct ttatctacca ggcacctttc
     1741 ccgatgggga aagacaatac cgaatactat ctactcactt ccgattacgt tagcgttgcc
     1801 gacttcgacg gcgaaaccat cctgaaagtg gaaccagaag ccctgaccct gctggcgcag
     1861 caagccttcc acgacgcttc tttcatgctc cgtccggcac accagaaaca ggttgcggct
     1921 attcttcacg atccagaagc cagcgaaaac gacaagtacg tggcactgca attcttaaga
     1981 aactccgaaa tcgccgccaa aggcgtgctg ccgacctgcc aggataccgg caccgcgatc
     2041 atcgtcggta aaaaaggcca gcgcgtgtgg accggtggcg gtgatgaaga agcgctgtcg
     2101 aaaggcgtct ataacaccta tatcgaagat aacctgcgct actcgcaaaa cgcggcgctg
     2161 gacatgtaca aagaggtcaa caccggcact aacctgcctg cgcaaatcga cctgtacgcg
     2221 gtagatggcg atgagtacaa attcctttgc gttgcgaaag gcggcggctc tgccaacaaa
     2281 acctatctct accaggaaac caaagccctg ctgactcccg gcaaactgaa aaacttcctc
     2341 gtcgagaaaa tgcgtaccct cggtactgca gcctgcccgc cgtaccatat cgcgtttgtg
     2401 attggcggta cgtctgtgga aaccaacctg aaaaccgtta agttagcaag cgctcactat
     2461 tacgatgaac tgccgacgga agggaacgaa catggtcagg cgttccgcga tgtccagctg
     2521 gaacaggaac tgctggaaga ggcccagaaa ctcggtcttg gcgcgcagtt tggcggtaaa
     2581 tacttcgcgc acgacattcg cgttatccgt ctgccacgtc acggcgcatc ctgcccggta
     2641 ggcatgggcg tctcctgctc cgctgaccgt aacattaaag cgaaaatcaa ccgcgaaggt
     2701 atctggatcg aaaaactgga acaccatcca ggtcagtaca ttccacaaga actgcgccag
     2761 gccggtgaag gcgaagcggt gaaggtcgac cttaaccgcc cgatgaaaga gatcctcgcc
     2821 cagctttcgc aatatccggt ttccactcgt ttgtcgctca ccggcaccat tatcgtgggc
     2881 cgagatattg cacacgccaa gctgaaagag ctgattgacg ccggtaaaga acttccgcag
     2941 tacatcaaag atcacccgat ctactacgcg ggtccggcga aaacccctgc cggttatcca
     3001 tcaggctcac ttggcccaac caccgcaggt cgtatggact cctatgtgga tctgctgcaa
     3061 tcccacggcg gcagcatgat catgctagcg aaaggtaacc gcagtcagca ggttaccgac
     3121 gcgtgtcata aacacggcgg cttctacctc ggtagcatcg gcggtccggc ggcggtactg
     3181 gcgcagcaga gcatcaagca tctggaatgc gtcgcttatc cggagctggg tatggaagct
     3241 atctggaaaa tcgaagtaga agatttcccg gcgtttatcc tggtcgatga caaaggtaac
     3301 gacttcttcc agcaaatcat caacaaacag tgcgcgaact gcactaagta acctcttcgg
     3361 cccagcgcct ggcagcatgc tgccaggtga tccccctggg ccacctcttt tgcgattgta
     3421 atttcacgct tgctggtgaa tagtcagtat tttccctgac ttgcgaactc accatgaccc
     3481 gcacactcaa gccgttaatt cttaacaccg gcgcactggc gctaacgtta atcctgattt
     3541 ataccggcat ttcggcccat gacaaactca cctggctgat ggaagtgaca ccggtgatta
     3601 ttgtcgtgcc gctactgctt gccaccgcta aacgttatcc gttaacgccg ctgctctata
     3661 cgctcatttt ctttcacgcc atcatcctga tggtcggcgg gcaatacacc tacgcgaaag
     3721 tccccatcgg ttttgaggtg caggaatggc tggggttgag ccgtaatccg tatgacaagc
     3781 tggggcattt tttccagggg ctggtgcctg cactggtggc acgagagatc cttgtgcgcg
     3841 ggatgtacgt tcgcggacgt aaaatggtgg cgtttctggt ctgttgcgtg gcgctggcga
     3901 taagcgcgat gtatgaatta atcgagtggt gggcggcgct ggcaatgggc cagggagcgg
     3961 atgattttct cggcacccag ggcgaccagt gggacacgca atccgatatg ttctgcgcgc
     4021 tgcttggcgc attaaccacg gtgatattcc tcgctcgctt tcattgccgt cagctacggc
     4081 gctttggctt gataaccgga taacaacgtg cactttgttg atgccggatg cggcgtgaat
     4141 tccttatccg gtctacaaaa tttgcaaatc caaagcattc cattaagacg tggcctgata
     4201 aacatatcgc atcaggccat tttcgttagc tattgtccac gtcaggcttt cacatcactc
     4261 actgcaccga ccggaatatc agcatgaaca ggctcgggcg gtattttgcg atatttatcc
     4321 agcagatgaa tctggatcct gcgcagcgtg tcgccattga ggcgatagaa acggaagtag
     4381 agaatcagcg ttaccatgaa aaacagagtc ggtagagcaa tcataataaa ctgcatacct
     4441 aatagggctt gcgtagactg ttcaacattc ggtacatagc caatcatccc taacacaacc
     4501 gcaatgaaaa aagccgcaaa ggctgaaccg cccttcacca ccatagtctg cacggagtaa
     4561 gcgatgcttt cacagcgtac gtgcagttta tattcaccgt aatcaacggt atctgccacc
     4621 atgatgacct gtaataccca gaaaagcgcc gttcccacat tcagcaaaat acccgcaatc
     4681 acaatgagga cgacgttgtg atagctcatt aatgccatta acaggagaac accacagctt
     4741 aacaccggaa gaatagatgc tccggcccat aaaatgcgtc gggataatga tttaactaag
     4801 cgtgggaaga atactaacgt caccaggtta gcagctcccg catacgacag ataatagggg
     4861 aacaaatccg catcaccgat aacatatgag aaataataga tagcaaagcc ggtaataatg
     4921 ttgctggcta cattataagc aagagccata cccaagaggc atgaaagctg atcgttttta
     4981 taaataagcg caacgatggc tttaagtgtc agatggcttc cttcagcaga cggttgattg
     5041 tctgacgaaa agacttcatg cacattgcgc agagtgatga tggttgaaac aataaaaaag
     5101 gcgatcagta ccagtgtgaa catctgaaag ccaaatcccc gatcgccacc gccgacataa
     5161 ttaacaaatg gtagcgtcac acctgccgta acaaagcccg ccagactggc aaaaaaacgc
     5221 ggataaggaa ccagttgttc acgctcacgt ttatcgagcg tgatggttgg aaccagcgac
     5281 cagaagggaa tatccataat ggtgtaagtc atgccccaga ggatgtaggt cacgcaaaca
     5341 aaggcaatct gagtagtacc ttcaaacaga tgcgcactaa agaggagaaa taagattaca
     5401 gagtttgcca acgtaccgat caggatccag ggtttgaact taccccatcg cgatcgcgta
     5461 gcatttacaa tccatcccat aatcggatcg ttaatagcat cccagatcct cgccaccaga
     5521 aacaaagtac cgaccaaacc cacagacagc ccgacgacat cggtgtagta atacatgagg
     5581 tacatataca caatgccgat cgcaaaatcc ttcccgaacg ctccaaatcc ataactgagt
     5641 tttgtagtca ttgaaatgct catagggtat cgggtcgctg ttaccaacgc ccgccttctg
     5701 ttattggtat cgtcagaggt gtgcgccgga ataaatcccc cggcgcagta gcgtttagtc
     5761 gcgttttaac ggtgcaacca gcctggcagc cagtcgccgt gggcggcaat caggtcgtca
     5821 acaagagcat atatttcgtc aatgcccagc acggcggcag tatgcgggtc catcatcgca
     5881 gcgtggtaaa cacggtcgcg attttccgta agaatagctt cggtcagcag cgtctgtacg
     5941 ttgatgttgg tttgcatcag ggcggccaga tgcgaaggta gcgtaccgac tttggtcggc
     6001 tgaatgccat tagcatcaac cagacaggcc acttccacgc aacatccttg tggcaggtta
     6061 tcaatcaaac catcgttacg gacgttgccg taaatcacac tcggctcgcc cgtccagata
     6121 gcgttcatga ttgtgctggc atattcccgt gacggtttaa tatcaatccg ggaggcgttt
     6181 ttatactcct ccagctcttt atgccagttc gccagctgct cgacgcagcg tttcgggtac
     6241 tcatccagcg gtactttata acgctcaatc aaatcctcac gacctggctt aataaaccac
     6301 ggtgtgtact cagcaaaatg ttctgacgat tccgtgacga aatagcccag ctttttgaac
     6361 atttcatagc gcacaatatt ctggcagcga gtattgccgt gaatattcgg cttcggtgcc
     6421 tgccctgcgt cataagccgc cagcagttcc gggtagagat tcacgtaact gccgtcggcg
     6481 gttttgcgct ccagctccag gtaaaacgcc atatggttga tacctgcgca acggtaacgc
     6541 agcgtagccg ggtcgatatt gaggtcacgc gccagctctt ccgccgttcc ctgcaccgaa
     6601 tggcacagcc cgacctgttt gatatgcgga tagcgggcat acatcgccca ggtattcatc
     6661 gccattgggt taacatagtt gagcatggtg gcatcggggc agacttccgt catgtcctcg
     6721 caaatttgcc acagatgcgg aatggtacgt agcgcgcgca taataccgcc cggccccaac
     6781 gtatcggcaa tggtttgttc cagaccatgc cgcttacaga cctcgaaatc agtcaccgtg
     6841 caaggtgcat aaccgccaat ctgaaatgcc accacgacaa aatcggcatc ctgtaaggct
     6901 tctttctgtt gggtgtggca ggtgattttg ccgctggccc ctgctgaatc catcagctta
     6961 cgcaccacaa tatgcgactc ttccaggcgg gtggggtcaa tgtccatcag ggcaatatgc
     7021 gccgttttca gcgcctcgcg atggaacaca tcaccaagaa tatttttaac gaaaatcgtc
     7081 gaaccagcgc cgataaatgt aattttgggt gcagacatca tgcagatctc ctggcttgct
     7141 tgaataactt catcatggca ggcgaatctg cagaaaacct ccggcttcca ggttgagcat
     7201 tcccataaac tcagatcctg cgtgaagcag cagtaaatct gagtttatgg gaataattta
     7261 cctgaaaagc agagttttcg gcctcatgat gctcggacac tcccctcgcc cctctgggaa
     7321 gagggtgaaa accgtgctcc cactcgcagt catcctccct cactcctgcc ataattctga
     7381 tattccagga aagagagcca tccatgaata cagatacgtt tatgtgcagc agcgacgaaa
     7441 aacagacccg cagcccgctg tcgctgtatt cggaatacca gcggatggag attgagtttc
     7501 gcgcaccgca tatcatgccc accagccact ggcatggtca ggtcgaagtg aatgtgcctt
     7561 tcgatggcga tgtggaatac ctgatcaaca atgaaaaagt gaatatcaat cagggacata
     7621 tcacgctgtt ctgggcctgt ataccgcacc aactaacaga taccggaacc tgtcagagca
     7681 tggcgatttt taatctgccg atgcatctgt ttctctcctg gccgctggat aaagacctga
     7741 ttaaccacgt cactcacggc atggtgatca aatcacttgc tacacagcaa cttagcccgt
     7801 ttgaagtgcg ccgctggcag caggaattaa acagtccgaa tgagcaaatt cgccagctcg
     7861 ccattgatga aattggcctg atgctcaagc gatttagcct ctctggctgg gaaccgattc
     7921 tggtcaataa aacctcgcgc acacacaaaa acagcgtctc gcgccatgcg caattttatg
     7981 ttagccagat gctgggcttt attgccgaaa actatgacca ggcgctgacc atcaacgatg
     8041 tggctgagca cgtcaaactt aacgccaact atgcaatggg gatattccag cgggtcatgc
     8101 aattgacgat gaaacagtac attaccgcga tgcgcatcaa ccacgttcgc gcgttactga
     8161 gcgataccga taaaagtatt ctcgatattg ccctgacggc aggctttcgt tcaagtagtc
     8221 gtttttacag cacgttcggc aaatatgtcg gcatgtcgcc gcaacaatac cgcaaactta
     8281 gccaacagcg ccgccagacg tttcccggct aaagacgata tcagtatcag ccaaaaaaat
     8341 agtttagccg
//
LOCUS       DBFIFI010000188         8333 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.188, whole genome shotgun sequence.
ACCESSION   DBFIFI010000188 DBFIFI010000000
VERSION     DBFIFI010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.188"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            177..605
                     /gene="uspC"
                     /locus_tag="N7B39_003904"
     CDS             177..605
                     /gene="uspC"
                     /locus_tag="N7B39_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HGU2423797.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCRKYH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(612..2036)
                     /gene="otsA"
                     /locus_tag="N7B39_003905"
     CDS             complement(612..2036)
                     /gene="otsA"
                     /locus_tag="N7B39_003905"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HGU2423798.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLV"
     gene            complement(2011..2811)
                     /gene="otsB"
                     /locus_tag="N7B39_003906"
     CDS             complement(2011..2811)
                     /gene="otsB"
                     /locus_tag="N7B39_003906"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HGU2423799.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDINVQLHTVIAQYPGAELETKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(2978..3964)
                     /gene="araH"
                     /locus_tag="N7B39_003907"
     CDS             complement(2978..3964)
                     /gene="araH"
                     /locus_tag="N7B39_003907"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HGU2423800.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVA
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(3979..5493)
                     /gene="araG"
                     /locus_tag="N7B39_003908"
     CDS             complement(3979..5493)
                     /gene="araG"
                     /locus_tag="N7B39_003908"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HGU2423801.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGMFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(5563..6552)
                     /gene="araF"
                     /locus_tag="N7B39_003909"
     CDS             complement(5563..6552)
                     /gene="araF"
                     /locus_tag="N7B39_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HGU2423802.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITSNELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKGVEPTKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            7349..7852
                     /gene="ftnB"
                     /locus_tag="N7B39_003910"
     CDS             7349..7852
                     /gene="ftnB"
                     /locus_tag="N7B39_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HGU2423803.1"
                     /translation="MATAGMLLKLNSQLNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(7932..8183)
                     /gene="yecJ"
                     /locus_tag="N7B39_003911"
     CDS             complement(7932..8183)
                     /gene="yecJ"
                     /locus_tag="N7B39_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HGU2423804.1"
                     /translation="MSQTLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
BASE COUNT         2171 a         2114 c         1908 g         2140 t
ORIGIN      
        1 cattattttc ttataattac agttgaaatc atatgatcac cgagagtgat tgattaattg
       61 tctttactaa taatcggact aatatttacc tgtttgactg agttgggatt gcgtcgtttc
      121 tccattagga gtaaagcttt aatgtcacct gaagttcaca gaataaggaa caggttatga
      181 gctatagcaa tattcttgtc gctgttgcgg ttacaccgga aagtcagcaa ctgctggcaa
      241 aagcggtatc tatcgccagg ccagtaaagg gacacatcag tttaattact ctcgcttccg
      301 acccggaaat gtacaatcaa ttagctgcgc cgatgcttga agatttacgc agcgtgatgc
      361 aggaagaaac gcaaagcttt cttgataagt taattcagga tgcggggtat cccgttgaca
      421 agacgtttat tgcctacggt gagttaagcg aacatattct ggaagtttgt cgcaaatatc
      481 atttcgattt ggtgatttgc ggtaatcaca atcacagttt cttttcgcga gcatcctgct
      541 cggcgaaaag agtgattgcc tcaagtgagg tcgatgtgct gttagttcca cttacgggag
      601 attaaccgct cctacacaag cttaggaaag gtagcaactt tatcgcgctg ctggctttcc
      661 gcgcttcgcg gaactatctg ctttaggtcg ctaatgaagc actcctgcca gtggttaata
      721 tcgtttttca cgataacgtc cagcatttct gcatgacggg aaatacgttc cgccagcgac
      781 atagtcaatg cacgatccag cgcagctgca acttcgtcac gatcgtaggg attaacaatt
      841 aacgccgacg ttaactcgtt tgccgctccc gcaaattgcg aaagaacaag aacgcccggg
      901 ttggctgggt cctgagcggc aacatactct tttgctacca ggttcatccc gtcacgcagt
      961 ggcgtcacta agcccacgtc agagtagcgg aatattttca tcagtaattt acggtcaaaa
     1021 tgctgattca aataataaag cggcgtccag cctaattgcc cgtatttacc attaattcgt
     1081 ccggcttcat tttcgagctg atgacgaata tcctgatagg cttgcacatc accacgcgac
     1141 gttggtgcaa tctgggtata acgaatttta ccatgatgct gcggatattt ttccagcaac
     1201 gcttcatagg cgagaaaacg ctctggcaaa cctttggaat aatccagccg ttcgacagaa
     1261 aagatatttt gtacgttttt cagttccgct ttaagttgcg ccagttttgg cggcagtggc
     1321 ccggcagcct gtttggctat ttcttttggc tcaatgccaa tcgggtaaac ttctgttcga
     1381 aacgctttgc cccaggctgt atggcttttt gcgctacgtg tcgtgacgcg ggtcaggtta
     1441 gaaaggcaat ccaggaacgc cagacgatcg ttttctgtct ggaaacccag caaatcataa
     1501 tcacaaagct gttcaagcaa ggtgtcatat gtcggcagcg cgttgaagat ttccggtgtc
     1561 gggaaaggaa tatgcagaaa gaaaccaatg cgattattca ctccccgttt gcgtaattca
     1621 tgcgcaaatg gcaacaggtg ataatcgtgg atccagataa tgtcatcgtc ttgcaacagc
     1681 ggcagtaatt tatctgccag caacgcattt acgcgtagat agccgtccca ggcaggacgc
     1741 tgaaattgca ccagatcgag gcgataatga aaagcaggcc agagaacggc attggagaat
     1801 tggttgtagt attcgtcaag gtcctgttcg ctgaggttaa aagaggccca cgtaatgtta
     1861 ccctttttca ccttttttag cggctgatcc tcattccctg tttcaccact ccagccaaac
     1921 cacagtccgc ctgcggcttt cagtgccccc agtatgccaa cggcaaggcc accggcactg
     1981 gcggcgtgct catctggtgg tgcaatccgg ttagatacta cgactaaacg actcatagtc
     2041 atcactcctg ttattttctc ttttttgttg taatgcggtg gttatcattt caagccagct
     2101 ccagacatcc ggtacgcccg ccagtcgcca tgatgcctga gttgcgcctg tgccaatttt
     2161 tactgacatt ccgcccagtc ggttaacgac tgcgaagcca gattcatcgg ttaaatcatc
     2221 gcccagaaat acgggcgttc gcccgataaa gggagcttcc tgcataaagg cagcaatcgc
     2281 ctcaccttta ctggtacctc tcggtttgat ctcgacaaca cactttccct gctgtaacgc
     2341 catttgtggc cagatctgag taatacgttg cgctaatgtc attaatgcgt cttcatgctg
     2401 cggagcctga cgataatgca gcgcaaaagc catccctttc gtctccagct ccgcaccggg
     2461 atactgggcg atgaccgtat gcagttgcac gttaatatca cgtgcaatcg catccggcag
     2521 atgaacgata tgcgttttac cattgatgtc acggcgctcc gccccatgca cgcccgctaa
     2581 cgggaagcga taaggttttg ccagtgcgtc cagctccacc attgagcgcc ctgatatcaa
     2641 tgccaatgca ccatcacttg cggttgccag tagctgaagt ccttgcagaa tattgtcagg
     2701 tacgacgacc tgatcgggat gcggtttgat ttccgccagc gttccatcga gatcaaaaaa
     2761 ccaggcatat ttcgcggata gttcaggggt ttcggttaac ggttctgtca cccggttctc
     2821 ctccttctct tttcttattg tgttgcgcag aacgcgcttt acagacatgt aagtatagac
     2881 agtgtgacgg gggtcgccat ttgcaacgaa gaacagccaa ccgttgtggc ggctggctgt
     2941 ggtgggaaaa aacgttaaat tgttgtggaa aaaagcatca gacagtgcgt ttcgcttttt
     3001 gcttgtaacg gtcgaagatc accgctgcca gcaggattaa gccgcgaacc acgtactgcg
     3061 cgaaaggaga aatattaagc aggttcatgg cgttttccac ggtgcctaaa attaagatac
     3121 ccgccaccac atatgagatt tttccgatgc cacctttcag agaaacgcca cctaaaacgc
     3181 aggcggagat aacaataagc tcataaccaa tcgacgtcat tggctgccca ctggtcatac
     3241 gtgaagccag aataattccg gctatcgcag ataccaggcc agagagaaca aagataataa
     3301 ttttggtgcg agcaaccggt acacccgcca gacgcgcagc ctcttcgttc ccgccaatcg
     3361 ccagggtatt acgaccaaag gtagttttat tcagcagcaa accaaagata atcagacacg
     3421 cgacggtgag ccagattggc gcaggcagac cgaaccagtt ggcgtaacca agggcaaaga
     3481 agctttcatc ttcgataccg accgctttac cgtcggaaat gatgtacgcc agacctcgaa
     3541 caatctgcat cgttgccagt gtcgtgatca gagcatttat tttcagtttg gcgataacaa
     3601 agccattgac caggccacag agaatgccaa gcagcaaccc cgctgccacg ccaatccaca
     3661 ggctttcagt cagattgata accaccgccg tggtgacacc cgcacaggca attacggagg
     3721 cgacagaaag gtcaaagtca ccggaagcaa ggcagaacaa catgccacac gccaccatcc
     3781 ccgacatgga aattgccagg cccaaccctt tcatattaat aaaggtggca aagttcggga
     3841 caaaaatggc acaggcgata aaaagcaccg caaacaccac cagcatgccg tactgatccc
     3901 agatacgccc gaagctgaat gacgacttag gtgcgccaga acccgatgta gaaacagaag
     3961 acatcatact ctccttactc aggcaactgc ctggctgact ttaggcatcg caaggctcag
     4021 tgcctgacgc tcatctgcct gctcgtgtaa caattcaccg gcgatttcac cttcgcgcat
     4081 caccacaatc cggtcggcaa cgccgaggac ttcaggtaag tcgctggagg caaacagcac
     4141 cgccacaccc tgcgccgcca gcgcataaat cacgttatat atttcgtgct tagcaccaac
     4201 atcaatgccg cgcgtaggtt catccagcaa aatgaccttc atctcttccg ataaccagcg
     4261 gcccagaatg gctttttgct gatttccacc tgagagattc ataatcagtt gctcagcgcc
     4321 cggcgttttg atgttgagcg aacgaatgtg gtgatcggca ttgttttctt cccaaccgtt
     4381 gttgattaca caaccgccga gcacatgttt acgtctggca ctgatgttga tattgtcgcg
     4441 aacggagtgc acaggaataa tgccttccgc tttgcgatct tccgggcaga gcatcatgcc
     4501 tgcggcaatg gcgtggctcg gtttacgaat atcgatcggc tgttggtcga tataaacctg
     4561 accggcggtg atttgcgtcc cgccaaacat gcctttcatt aattcgctac gccccgcccc
     4621 taccagacca aacagcccaa caatttcacc actgcgaacc gccagactta ttggcgtacg
     4681 cacgcctggt gctttcacag catcaagacg caggcgctcc tcgccataac tacgcggttg
     4741 ccagccgtag atatcgccaa tgtcgcgccc gaccatcgcc tgcaccagcg cgtcgtggtc
     4801 aacctgctgc atatcggtaa aggttttgac ataacgtcca tctttaaaga cagtgatggc
     4861 atcgctgagg gcaaatattt cttccatacg gtgagaaacg tataagatca cccgcccctc
     4921 ttttcgcagt tcacgaataa cgcggaaaag attgtcgatt tcgcgggcag aaagggagct
     4981 ggttggctca tcaaaggcga taattttagc gttacgcgcc agcgccttgg cgatttcaac
     5041 catctgccac tgaccaatgg agagatattt cagcggcgtg tccgggtcaa tatccatacc
     5101 aagatgttta agttgtaaac ccgcctcata attcagcaat gagcgattca caatgccgcc
     5161 tttatgcggc agctggccga gatagatgtt ttccgcgacg gtcatttccg gcacgagatg
     5221 cagttcctgg taaataatcg ccacacccgc gttaagtgct gcggtcgtgt cggaaaagga
     5281 catttcctgc ccattaatca ctacagaacc cgtggttggc gcatagttgc cgctgaggat
     5341 ttttaagaga gttgattttc ctgcgccatt ttcacccatc aacgcatgaa cctgaccggc
     5401 atagcagtca aaactaatat ccgtcagcgc cttaacgccg ggaaacgttt taccgatgcc
     5461 gcgaaatgag agatacgggg tagactgttg cataacgtct ccgtgaatca ctggtcgttc
     5521 acaccccctc tgcatcatgc aaaggggaat ttttccggca aattacttac cgcctaaacc
     5581 ttttttctcc agttcttctt taaagttgtc acgcgtgatc agcaccacgt cggtaacttc
     5641 ggtgaatttg gtcggttcaa caccttttgc tacccagttg taaagcattt cgctggactt
     5701 ataaccatgt acgtccgggc ttggcaacag ggaaccgtag aagccggttg cctgtgcctt
     5761 agacagttcg ctcaccgcat ccacaccgtt aataccgata ccgatgatat cggccgcttt
     5821 aaagccctga ccttccgtcg cgcgtacgcc gcccagcacg gtgctgtcgt tcataccgac
     5881 gatcagccaa tgtttaactt ccggatgttg aaccagcatt gagttggcag cgtcaaatgc
     5941 cccggggatg tcgttagatt tggtaggtac ctgataaatt tgtttttccg ggaatcctgc
     6001 agctttcagc gcatccatag aacccgtagt acggcgacga gccgtatcca gttcgttgga
     6061 ggtaatcgcc atcaccgcgc tttctttgac atcccagcca cgtttctgca tctctttata
     6121 cagttcctgg ccctgacgtt cgccaatttt ggtcgccgcc atcatcacca gaggaacggt
     6181 atccattggc ttacctttgg cgttaacaaa ctggtcatcc acggcaatga ctttcatatc
     6241 gtagccacgc gctttcgcca cgatggcaga gccgagtttc gggtccggag tacaaataac
     6301 gaaacctttt gcgccactgg cggccaggct atcgatcgcg ttcaatgttt tttcgccatc
     6361 cggcacggca atcttaataa cctcaaaccc taaatccttc ccggctttat cggcaaactt
     6421 ccattcggtc tggaaccacg gctcttccgg ttgcttcacc aggaaaccga gcttcaggtt
     6481 ctccgccata gcggattgtg acataacggc tgccagacca atggctgcca gggctttagt
     6541 aaatttgtgc atggttctct ccagctttag tgtcgttttg tgcagggcaa aaacgaatga
     6601 cattcgttaa attaatcgga aaacaaagca ttacctttta actaaaagat aagtgactgt
     6661 gttgacatag ttttagcgag aaattaattc tccataggag agcaatatca catcgcagaa
     6721 ttacagtgag aacgtgcata aatttagcgg gaaaagacat aaggaaaagc caatttgtca
     6781 gacaaattgt tgaatgcaca gcagattaat ccataagatt agcctggaaa tccttgttgt
     6841 ctttggtacc catgcgggat gtcttctttt tcaccagtca ataggccgca ttacctggcg
     6901 ttgagttttt gaaatggtgt aataaccgca actcaaagat gtggaaaatg cacgtcattc
     6961 atttcgtcat taattatcac tgtgctcatt aattaacaga acacgtataa tgagagccat
     7021 ctcgcaaaaa tgaaaaaacg ttttataaaa taatcacttc atcatgaatt caaattcatt
     7081 gattaatatc aacaagatac aaaaagcact atcattaaaa ttcattgcag ttacattgat
     7141 ttcatcaatg aaatgtaaaa atatataaac ttgatgattt aagcattttc ttatacccgt
     7201 tcagacgtta ttcttatttc agatcatcgt cagaattgac tccacgatca catttcggac
     7261 cggcagaaag gaattattct gcaaacagta attatggtgt tttgatttat cttgcacctc
     7321 tccacttctg gatataagga tattaggtat ggcaaccgct ggaatgcttc tcaaactcaa
     7381 ctctcaattg aaccgcgagt tttacgcatc caatctctac cttcacctga gtaactggtg
     7441 ttctgaacag agtctgaacg gcaccgccac tttccttcgc gcccaggcac agagtaatgt
     7501 gacccaaatg atgcgcatgt ttaactttat gaagagtgtc ggcgctaccc ccatcgttaa
     7561 agccattgat gttcccggtg aaaaactgaa ctctctggaa gaactgttcc aaaaaacgat
     7621 ggaagaatac gagcaacgtt ctagtacgtt ggcacagtta gccgatgaag cgaaagaact
     7681 gaatgatgat tcaaccgtca atttcctgcg cgatctggaa aaagaacagc agcatgatgg
     7741 cctgttgctg caaaccattc ttgatgaagt gcgcagtgcg aaacttgcgg gtatgtgccc
     7801 agtgcagacc gaccaacatg ttctgaatgt cgtgtcgcat cagctgcatt gatcatacat
     7861 cgacgctaat gcattgcacc gatgaagttt ttgagaaacc gctgcctcat cttttttgaa
     7921 gcagcggttt tttaatggga ttcgccttgt ggggtaaact tgagctcaat cagcgcgatg
     7981 gctttttgga ttgcccgcat ggtgaccggg tctgcggcag cgggatgatg agtaaagtcg
     8041 atattcttca gctgactgga cattttttca cgaacttcaa cgggcgcgat tacatcgaga
     8101 acatccagaa tttgtttgat aaccaactgg caagcaacca catcagaaac cagttcctga
     8161 tcggcattca gcgtctggga catcgtaaac tcctgatagc attttgaaag ccgttatagt
     8221 agcggcttca catcttcaac gatagtcaca tccaccctca tcaggacaca aaaaaacctg
     8281 ccggagcagg ttttttgtta tcggaacata ttgcctggcg gtacgtcttt gaa
//
LOCUS       DBFIFI010000189         8297 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.189, whole genome shotgun sequence.
ACCESSION   DBFIFI010000189 DBFIFI010000000
VERSION     DBFIFI010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8297
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.189"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(19..399)
                     /locus_tag="N7B39_003912"
     CDS             complement(19..399)
                     /locus_tag="N7B39_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001292087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2423805.1"
                     /translation="MSELEKITIPNGAAFPGTDISSLEDTPPTTESSSDGDNLNAQRA
                     ADAKSNRELREKFAEKAYKVAKYGLYWWASVLLLSATGRVMGKEIFSDEVLIAVTAAS
                     TLNLFAAFLGVIRGLFPHAKSHTE"
     gene            complement(399..869)
                     /locus_tag="N7B39_003913"
     CDS             complement(399..869)
                     /locus_tag="N7B39_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Panacea domain-containing protein"
                     /protein_id="HGU2423806.1"
                     /translation="MAYSAAAVANAFIDKAKRGEIKDLTPMKLQKLLFYTQSWHLKLF
                     NGRPLFDDLFARWPYGPVIPHLYHMLKKYGGDEIQEMISIIIDSDGEWKRITPTIQKG
                     DTETRRLIDRIVEVYGSYSGTELSNMTHAPNAAWSKGQPDGGPILHTDMANHIH"
     gene            1087..1335
                     /locus_tag="N7B39_003914"
     CDS             1087..1335
                     /locus_tag="N7B39_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HGU2423807.1"
                     /translation="MGKADKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRF
                     FNAEINHTILMHRPHPESYVKGGTLKAIKQNLKEAGVL"
     gene            1332..1676
                     /locus_tag="N7B39_003915"
     CDS             1332..1676
                     /locus_tag="N7B39_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HGU2423808.1"
                     /translation="MKHLKYKGYLGTVEPDFENNILYGKLAFIRDLVTYEASTLAELE
                     KEFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPELHRRVAEMAMEEDLSLNAFVNK
                     ALEKEVNHHTGA"
     gene            complement(1763..1924)
                     /locus_tag="N7B39_003916"
     CDS             complement(1763..1924)
                     /locus_tag="N7B39_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000708858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transmembrane anchored protein"
                     /protein_id="HGU2423809.1"
                     /translation="MKKAFAALFVLLSLVASTQSFAGNCQHDNDTAADGSRCGGRSSD
                     SRPGGGGIR"
     gene            complement(2012..2731)
                     /locus_tag="N7B39_003917"
     CDS             complement(2012..2731)
                     /locus_tag="N7B39_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HGU2423810.1"
                     /translation="MTKKITITDKDIQNAERLRKIWDEKRKQLSLSQEKAADILGFKT
                     QGAVSQLLNAKIALNTENTLKFAALLQVPAEEINPDLSDLLRSIRTHTPGNRRNLFEK
                     YYEYPLLSCVQAGAFSMDDFSYTAKDAIKWISTTTKASDRSFWLEVKGHSMTAPQGGK
                     PSFPEGMLILVDPEREIEDGDFCVARMNGDEFTFKRFIRESGKAYLEPLNPRFDMIEC
                     NENCQFVGKVIKSQWNDETFD"
     gene            2837..3076
                     /locus_tag="N7B39_003918"
     CDS             2837..3076
                     /locus_tag="N7B39_003918"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU2423811.1"
                     /translation="MTLDEYLRQSSIRQADFAAIVGVSQGFVSGVITGRYMPQGRKAI
                     EWAKATDWNVTPHELNPHDYPNMEDGIPLHVRQTA"
     gene            3123..3380
                     /locus_tag="N7B39_003919"
     CDS             3123..3380
                     /locus_tag="N7B39_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001115081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2423812.1"
                     /translation="MPDQYSDADRDWIQEQLLQLDPTTRVKIAAEYARVYQEEWDKEP
                     VSFRKGNRARRSANTRLRVYVQKYARASRGWMISPVAIRKE"
     gene            3600..3986
                     /locus_tag="N7B39_003920"
     CDS             3600..3986
                     /locus_tag="N7B39_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2423813.1"
                     /translation="MFIKHVVINLLGNKFYTGKDKVTFDYVLAAKLRDAGLAIERNYL
                     VDMGNGKRGFVDIVVVAPSGERCAIEVDRASPRARSILKLRRLKLYGIPGIVLLRCSR
                     NPDQYVCDEIDVIPATGKSRSKGASC"
     gene            3980..5500
                     /locus_tag="N7B39_003921"
     CDS             3980..5500
                     /locus_tag="N7B39_003921"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGU2423814.1"
                     /translation="MLKITPNFAQERGLNQLRHQWKQHRTYLMYAPTGSGKTGLAAFV
                     TAGMVERGMRVMFVCPYTILLNQTAERFTEYGLPWEEISFVWRDHPNYDPSRLIQIAS
                     ADTLIRREFPDNIDLLIIDEAHMKRRALLEVIRDKDIRVLGLSGTPFAAWMGKYYECL
                     IKPTTIRELIQRGDLSDYEFFAPSMPDLAGVKTSNTVFGRDYNEEQLASIMGSSDLVG
                     DIVSNWLENGEDLPTICFCVNVAHANFVTREFLQSGIGAEVMTADTPHDERQDIIRRF
                     EEGATKIIVNVGVLVAGFDSDVRCLIYARPTKSEIRWLQCIGRALRTASGKKRALIFD
                     HSGTVHRLGFPEDIEYDELPGKNDGMKASAGGGEVKAEKLPRECSRCHFMKPAGVHIC
                     PKCGFRPLGGDDVATDRDRKLSRVNKGKREYSREEKQRWWSEIKGYQNYRNATGKPLS
                     DGWCAHTYKEKFGVWPKGFSNAPLQTSVEVYNFIKSKTIAYAKGRKKAMTGGQHAD"
     gene            5490..6461
                     /locus_tag="N7B39_003922"
     CDS             5490..6461
                     /locus_tag="N7B39_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primase-helicase zinc-binding domain-containing
                     protein"
                     /protein_id="HGU2423815.1"
                     /translation="MQTKKAAVGHWGRIFEYYGMPPVTGLKHYSGPCPICGARGKFRC
                     DDKDGSGSWICVCGHGDGMKLLHLATGKPWVTLCDEIDRLIGNTWKREKVSQPVTEIS
                     RKRELVMDKFAGLPCLRGTTGEAYLQGRGILQLPTESVRFCDRQIASGREYQAIYAIA
                     TDDRGSLCYLHRTLLDGDRKANVEAAKKMTALQELPGLQHAKSVAIRLYPVSSTLGIA
                     EGIETALSCRQIFRCNVWSTMNSGFMEKFIAPPGVNHLIIFADNDEHGAGLAAAFKCG
                     HKNLMSRNDVEKVSIRWPDLPDFNDMLIQGCEAREHVLTRKFKAEAA"
     gene            6461..6910
                     /locus_tag="N7B39_003923"
     CDS             6461..6910
                     /locus_tag="N7B39_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HGU2423816.1"
                     /translation="MEIEMIKAANGVFVPAYERDLPRLAKFKNGELYTLEAKLTRNPS
                     FHRKMFAFLNFCFQYWCAEHAGYEFSDEATQFDEFRKNLTILAGFYDVVTTIRGEVRY
                     RAKSLSYANMDQDEFERCYNAMINAALKHVFGRSNNPELNNRLLSFF"
     gene            6918..7481
                     /locus_tag="N7B39_003924"
     CDS             6918..7481
                     /locus_tag="N7B39_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HGU2423817.1"
                     /translation="MKQRKPKKCKVCGSSFVPFRSYQKVCCGQCALELVRKEKAIASA
                     KEQADKLKARRRDLQPRSYWIKQAQQAVNAYIRERDRHLPCVSCGTFDSAQWDAGHYR
                     TTAAAPQLRFDERNIHKQCVVCNQYKSGNLVPYRVELINRIGQEAVDEIESNHSRHRW
                     TVEECKAIKAEYQQKLKDLRNSRSEAA"
     gene            7478..7672
                     /locus_tag="N7B39_003925"
     CDS             7478..7672
                     /locus_tag="N7B39_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HGU2423818.1"
                     /translation="MTFTVKTIPDMLVEAYGNQTEVARILNCNRATVRKYIGDKEGKR
                     HAIVNGVLLVHRGWGKDTDA"
     gene            7665..8099
                     /locus_tag="N7B39_003926"
     CDS             7665..8099
                     /locus_tag="N7B39_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HGU2423819.1"
                     /translation="MRDIRQVLERWGAWAANNHEDVTWSPIAAGFKRLIPEKVKSRPQ
                     CCDDDAMIICGCMARLNRNNSDLHDLLVDYYVLGETFMALARKHGCSDTCIGKRLHKA
                     EGIVEGMLMMLGVRLEMDRYVERELPGGRTSVFYQRKNSLRS"
BASE COUNT         2245 a         1811 c         2162 g         2079 t
ORIGIN      
        1 aaccggcgcg ggggccggtt actctgtgtg agatttggcg tgagggaaaa gccccctgat
       61 aacaccaaga aaagcagcga acagattcag agttgaagca gcggtcaccg caatgagcac
      121 ttcatctgaa aagatttctt tccccatgac gcggcctgtt gcagacaaca acaaaacact
      181 agcccaccag taaagcccat acttggcaac tttataggct ttttctgcaa atttttctct
      241 taattccctg ttagattttg catctgctgc gcgctgggcg tttaaattat ctccatctga
      301 tgaagattct gttgttggtg gcgtatcttc aaggcttgat atatctgtgc ctggaaatgc
      361 tgctccgttc gggattgtga ttttttcaag ttcagacatc aatgaatgtg gtttgccata
      421 tcagtatgaa gaattgggcc accatcaggt tgacctttac tccaggccgc gtttggtgca
      481 tgggtcatgt tagaaagctc ggttccgctg tagctgccgt atacctcaac aattctgtcg
      541 atcagcctcc tggtttctgt gtcacccttc tgtattgtcg gtgtgattcg tttccattcc
      601 ccatctgaat caatgatgat gctgatcatt tcctgtattt catcgccccc atatttcttt
      661 aacatgtggt acaggtgcgg tattacaggc ccgtaaggcc agcgcgcaaa cagatcgtcg
      721 aataatggcc ttccgttgaa tagtttcaga tgccacgact gagtatagaa caacaatttt
      781 tgtagcttca tcggagttaa atctttgatt tctccccgtt tagccttatc aataaaggcg
      841 ttagctacgg cagcagctga atatgccatg ctctactccg cattttgcga aattaaatgt
      901 atcaccggag agaatacaac aaaaagcaaa ttagcaaaac tgatagagaa agtcggaagc
      961 aaaaaaacca agaagcgaac agcagtaacg agtcaaatag aaccctgcgg ggttggtggt
     1021 ttttgacaaa aacactgcat gtaactataa ttagttacag tgtagcgaag atgagattat
     1081 gtgggcatgg ggaaagcaga taagctactg gcaaaatttt taaacagtaa aaaaacgttt
     1141 gaatgggatg agctggtcgt tttgttttcc tctctgggat atgtcaaaaa ggaaatgcag
     1201 gggtcaagag tgcggttttt caatgctgaa atcaaccaca ccatattaat gcatcgccct
     1261 catccggaaa gttatgtcaa aggtggcacg ttgaaagcga ttaaacagaa tctgaaagag
     1321 gctggggtat tatgaagcat ctaaaatata aaggatattt aggtacagtt gaaccagatt
     1381 ttgaaaataa tatcctctat ggaaagctgg catttattcg ggacttggta acttatgagg
     1441 cttctacgtt agctgaactg gaaaaggagt ttaaaacatc agttgatctg tatttacagt
     1501 cttgtgtgga ggatggaaag gaacctgata ccccgtttaa aggcgtgttt aacgtccggc
     1561 ttgatcccga actgcatcgt cgggtagcag aaatggcgat ggaagaagat ttatcactga
     1621 acgcatttgt taataaggcg ctggaaaaag aagtcaacca tcacacaggg gcttaatgtc
     1681 accttcgtct ttgacatcct caccgacatg aagaactggt tttacggcac accggataaa
     1741 ggccgcgaaa gcggccttgt ttttagcgaa ttccaccacc gccaggacga gaatcagaag
     1801 aacggccacc acaacgagag ccgtcagcag cagtatcatt gtcgtgctgg caattacccg
     1861 cgaatgattg agtcgaagct accagtgaca acaagacgaa caatgcagca aatgcttttt
     1921 tcatgtaaga caccttattt taggttttgg taagctcaaa taatgcccga aaagtgcatc
     1981 cctgcaaggt tattgatatc gctctgtgag ctcaatcaaa agtctcatca ttccactgcg
     2041 actttatgac ttttccgaca aactggcagt tttcgttaca ctcaatcatg tcgaatcgtg
     2101 ggttgagtgg ctccaggtac gctttaccgc tctcacgaat gaatcgtttg aaggtgaatt
     2161 catcgccgtt cattcgggcc acgcaaaaat cgccatcctc gatttcccgt tcagggtcaa
     2221 cgagtatcag catcccttca gggaaactgg gtttaccacc ctgcggcgcg gtcattgaat
     2281 gccccttgac ctccagccag aacgacctgt cactggcttt tgtggtggtg gagatccact
     2341 taatcgcatc ctttgcggtg tacgaaaaat catccattga gaaagctcca gcctgtacgc
     2401 aggagagtaa cggatattcg taatatttct cgaacaggtt acgcctgttt cccggtgtgt
     2461 gggtacgaat actgcgtaac aaatcactca gatctggatt aatttcttcc gcaggaacct
     2521 gtaataaagc cgcaaactta agggtatttt cagtatttaa cgcaattttt gcgttcaata
     2581 actgactaac agccccctgc gttttgaagc cgaggatatc tgcggctttt tcctggctta
     2641 gggatagctg tttccgtttt tcatcccaga ttttgcgcag tcgttcagcg ttctggatgt
     2701 ctttgtcagt aattgtaatt ttcttcgtca tagtgcctcc aattttatta gcaacacaaa
     2761 tatttagcaa atagctagcc tattgacatt taaatcaggt aagctaatat cggaaaattg
     2821 aaatgggagg atctctatga ctcttgacga gtacttgaga caaagctcta tcagacaagc
     2881 cgattttgcg gcgattgttg gagtttctca gggatttgtc agtggggtta ttacagggag
     2941 atacatgcct caggggcgga aagccattga atgggcaaaa gctacagact ggaatgtaac
     3001 tccccatgaa ctaaacccgc atgactaccc aaacatggaa gacggtattc cgcttcatgt
     3061 aaggcaaaca gcctgataac cctccatcgg aggtgtgagt gaacaggcaa atcaaaacgg
     3121 ttatgcccga tcagtacagc gatgctgatc gggactggat acaggagcaa ttgttacagc
     3181 tagacccgac cacccgggta aaaattgcag cagagtacgc gagagtgtac caggaagagt
     3241 gggacaagga gcctgtatcg ttcaggaagg gcaacagggc aagacggagc gccaacaccc
     3301 ggttacgggt gtatgtccag aagtacgcga gagccagccg tggctggatg atttcgccag
     3361 tagcgatacg gaaggaatag acctcatcaa ggtgactatt ttttgtgaaa taattgcata
     3421 tgaaattatt ttacctgaaa ttttactaag aagggggtaa gggggcagat ctgtaacatc
     3481 cgtgcccgta agggcactac cagaaagaca gctccatagg ttaggtagat cactgtatag
     3541 ggtgcgatag tttctggcaa aattattatc atgctaacta ttacagtgga aatagtacca
     3601 tgttcatcaa acatgtggtt atcaacctgc tcggaaacaa attctacacc ggaaaagaca
     3661 aggtcacgtt tgattatgtg ctggctgcca aattgcgtga tgccggactg gcgatcgaac
     3721 gtaattatct ggttgatatg ggcaacggta agcgcggatt cgtcgatatc gtggtcgttg
     3781 ccccatctgg tgaacggtgc gcgatcgagg tggacagagc gtcaccacgg gcacgttcaa
     3841 tactgaaact gcgcagactc aagctatacg gcattcctgg tatcgtgctt ctgcgctgtt
     3901 cacgtaatcc ggatcaatac gtttgcgatg agatcgacgt gatcccggca acgggtaaat
     3961 cacgtagcaa gggggcatca tgctgaaaat caccccaaac tttgctcagg agcgcggttt
     4021 aaaccagctg cggcaccagt ggaaacagca tcgcacgtac ctgatgtacg cacccactgg
     4081 ctctggcaaa actggtcttg cagcattcgt caccgccgga atggttgaac gtggtatgcg
     4141 ggtgatgttc gtctgtccgt acaccatcct gctgaaccag acagcggaac gtttcaccga
     4201 atacggtttg ccgtgggaag aaatcagttt tgtatggcgt gatcacccta actatgaccc
     4261 gtcacgactg attcagattg ccagtgccga cacgctgatc cgtcgtgagt ttcctgacaa
     4321 catcgatctg ctgatcatcg atgaagcaca catgaagcgt cgtgcgttac ttgaggttat
     4381 ccgggacaaa gatatcaggg ttcttgggct ttccggcacc ccgttcgcag cctggatggg
     4441 gaagtactac gaatgcctga tcaagcctac aacgattcgt gaacttattc agcgtggtga
     4501 tctgagcgat tatgagtttt ttgccccctc aatgcctgat ctggctggcg taaaaaccag
     4561 taacaccgta tttggacggg attacaacga ggaacagctc gcatccatca tggggagttc
     4621 ggatctggtt ggcgatatcg tcagcaactg gcttgagaat ggcgaggatt taccgacgat
     4681 ctgcttctgc gtgaatgtgg ctcatgcgaa ctttgttacc cgtgagtttt tacagtctgg
     4741 cattggcgca gaggtgatga cggcagatac cccacatgat gaacgacagg acatcattcg
     4801 ccgttttgaa gagggggcga ccaaaataat tgtcaacgtt ggtgtacttg ttgctggatt
     4861 tgacagtgat gttcgctgcc tgatctacgc ccgtccgacc aaatcagaaa tccgctggtt
     4921 acagtgcatc gggagagcgt tacgtactgc ctctggtaaa aaacgggcgc tgattttcga
     4981 ccattccggc acggttcatc ggctgggctt ccctgaggat atcgagtatg acgaactgcc
     5041 gggcaaaaat gacggcatga aagcgtctgc tggcggtggt gaggttaaag ctgaaaaact
     5101 tcccagagaa tgctccagat gccacttcat gaagcctgct ggcgtgcata tatgccctaa
     5161 atgtgggttt cgtcctcttg gtggcgatga tgttgcgacc gaccgcgatc gaaaactctc
     5221 acgcgtaaac aaagggaaac gcgaatacag tcgcgaggaa aagcagcgtt ggtggagtga
     5281 gatcaagggg tatcagaatt accgcaacgc aacaggtaaa cccctgagtg acggatggtg
     5341 cgctcatacc tacaaggaga agttcggtgt gtggcctaaa gggttcagta acgcgccgct
     5401 gcaaacctca gttgaagtgt acaacttcat caagtcaaag accattgcct atgccaaagg
     5461 gcgcaagaaa gcaatgacag gaggccagca tgcagactaa gaaggcagcg gtcgggcatt
     5521 gggggcgaat ttttgaatat tacggcatgc ctcctgttac tggcttgaaa cattatagcg
     5581 gcccctgccc gatatgtggt gccagaggta aatttcgttg tgatgataag gatggatccg
     5641 gctcgtggat ttgcgtctgt ggtcacgggg atgggatgaa gttgttgcac cttgccacgg
     5701 gtaagccctg ggtgacgttg tgtgatgaaa tcgatcggct gatcggaaat acctggaaga
     5761 gggagaaagt cagccagcct gtaacagaga taagcagaaa gcgggagctg gtcatggata
     5821 agtttgccgg actcccgtgt ctgcggggca caacaggtga ggcgtacctg caggggaggg
     5881 gaatactcca gttaccgacc gaatccgtgc gtttttgtga ccgtcagatc gccagcgggc
     5941 gcgaatatca ggcaatttac gccattgcaa cagatgacag aggttctctt tgctatctgc
     6001 atcgtacgtt gctggatggt gatcgcaagg cgaatgtaga ggcggctaaa aaaatgaccg
     6061 cgctacagga gttgcctggt ttgcagcatg ccaaatcggt ggcaatacgc ctgtatccgg
     6121 tgtcgtccac tctggggata gccgagggta tcgaaactgc gctttcatgc cgtcaaatct
     6181 tccgctgcaa tgtgtggtca acaatgaact ccggttttat ggagaagttt attgcgccac
     6241 caggcgttaa tcacctgatt atctttgcag acaatgatga gcacggcgca ggtctggcgg
     6301 ctgcctttaa atgtgggcat aagaatctca tgagtcgtaa tgacgttgag aaggtcagca
     6361 ttcgctggcc tgacttgccg gattttaacg acatgctcat tcaggggtgt gaagcccgtg
     6421 aacatgtgtt gacgcgcaaa ttcaaagcgg aggctgcctg atggaaatag agatgatcaa
     6481 ggcggctaat ggcgtatttg taccggcgta tgagcgcgat ttaccccgac tggcaaaatt
     6541 taaaaacggt gagctgtata cactggaagc aaaacttacc cgtaacccat cttttcaccg
     6601 gaagatgttc gcttttctta atttctgctt tcagtactgg tgcgctgaac atgctggata
     6661 cgaattctct gatgaagcga cgcagtttga tgagtttcgt aaaaatctga caattcttgc
     6721 cgggttctat gatgtggtca caaccataag aggcgaggtg agatatcggg caaaaagcct
     6781 gagttacgcg aatatggatc aggatgagtt tgaacgttgc tacaacgcaa tgattaatgc
     6841 cgcgttaaaa catgtgttcg ggcgctcaaa caatcctgaa ctgaataacc gcctgctgtc
     6901 gtttttctga ggtgatgatg aagcaacgta aaccaaaaaa atgcaaagtg tgcggctcct
     6961 cgtttgtgcc gttccgctca tatcagaaag tttgctgtgg tcagtgcgca ctggaactgg
     7021 tcagaaaaga aaaggcgata gcttcagcaa aagagcaggc agacaagctg aaagcgcgca
     7081 ggagggactt acagccccgc agttactgga ttaagcaggc acaacaggct gtgaatgctt
     7141 atatcaggga gcgggaccgt catttgccgt gtgtttcatg cgggacgttc gattcagccc
     7201 agtgggatgc aggccattac cgtacaacag ctgcggcacc tcagctcaga tttgatgaac
     7261 gcaatatcca taagcaatgc gtggtgtgta accagtacaa aagcggaaat ctcgttccgt
     7321 atcgtgtcga actgattaac cgcatcgggc aggaagcagt agacgaaatc gaatcaaacc
     7381 atagtcgcca ccgctggact gtcgaagagt gcaaggcgat caaggcagag tatcaacaga
     7441 aacttaaaga cctgcgaaac agcagaagtg aggccgcatg acgttcaccg taaaaaccat
     7501 tcctgacatg cttgttgagg catatggaaa tcagaccgaa gtggcccgaa tactgaactg
     7561 caatcgtgcc acagtcagaa aatacattgg cgataaagaa gggaaaagac acgctatcgt
     7621 caacggtgtt cttctggttc accgcggatg gggtaaagat actgatgcgt gatatccggc
     7681 aggttcttga gcgctggggg gcatgggcgg caaataacca tgaggatgtt acatggtcgc
     7741 ccattgctgc cggatttaag aggctgatcc ccgaaaaagt aaaatcacgt ccacagtgtt
     7801 gtgacgatga cgcgatgatt atatgcgggt gtatggctcg ccttaacagg aacaacagcg
     7861 atctgcatga cttgctggtt gattattacg tgttggggga gacgttcatg gcgctggcac
     7921 ggaaacatgg gtgctctgac acctgtatag gtaaacgcct tcacaaagcg gaggggattg
     7981 ttgaaggcat gctgatgatg ctgggagtga ggcttgagat ggatcggtat gttgagcgtg
     8041 aattgccggg agggagaacc tctgtatttt atcagcgaaa aaatagttta cgatcgtaaa
     8101 aatctgcata tcatgataag agtggttaca ttgccacgca ttcgaacccg ccgatgcgcg
     8161 ggtttttttg taccccgaat cctgtgagct atacggaaag tacacagaaa ggaaggtgcg
     8221 accgtaatta ataacaaaat cttaaaaatc gcatatagca ctattagttt tctaaatatt
     8281 gtatatttta agtattg
//
LOCUS       DBFIFI010000190         8159 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.190, whole genome shotgun sequence.
ACCESSION   DBFIFI010000190 DBFIFI010000000
VERSION     DBFIFI010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8159
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.190"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            131..1108
                     /gene="glaH"
                     /locus_tag="N7B39_003927"
     CDS             131..1108
                     /gene="glaH"
                     /locus_tag="N7B39_003927"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HGU2423820.1"
                     /translation="MNALTAVQNNAVDSDQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGV
                     DDVKQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPMARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIEISKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            1128..2396
                     /gene="lhgO"
                     /locus_tag="N7B39_003928"
     CDS             1128..2396
                     /gene="lhgO"
                     /locus_tag="N7B39_003928"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HGU2423821.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSELEMERM
                     RALWERTAANGIEREWLNADELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSGLSEHKSGVVIRTRQGSDYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            2419..3867
                     /gene="gabD"
                     /locus_tag="N7B39_003929"
     CDS             2419..3867
                     /gene="gabD"
                     /locus_tag="N7B39_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HGU2423822.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPVWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKAHERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            3881..5161
                     /gene="gabT"
                     /locus_tag="N7B39_003930"
     CDS             3881..5161
                     /gene="gabT"
                     /locus_tag="N7B39_003930"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HGU2423823.1"
                     /translation="MSSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAT
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHNKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            5399..6799
                     /gene="gabP"
                     /locus_tag="N7B39_003931"
     CDS             5399..6799
                     /gene="gabP"
                     /locus_tag="N7B39_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HGU2423824.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYADVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            6820..7482
                     /gene="csiR"
                     /locus_tag="N7B39_003932"
     CDS             6820..7482
                     /gene="csiR"
                     /locus_tag="N7B39_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HGU2423825.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7483..7932)
                     /gene="kbp"
                     /locus_tag="N7B39_003933"
     CDS             complement(7483..7932)
                     /gene="kbp"
                     /locus_tag="N7B39_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HGU2423826.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8016..>8159)
                     /gene="yqaE"
                     /locus_tag="N7B39_003934"
     CDS             complement(8016..>8159)
                     /gene="yqaE"
                     /locus_tag="N7B39_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HGU2423827.1"
                     /translation="IVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIHAFWVQ
                     TRD"
BASE COUNT         1815 a         2135 c         2326 g         1883 t
ORIGIN      
        1 ctttttatca caataagaaa acaatatggc gcatttgtgc gcatttttca gaaatgtaga
       61 tatttttaga ttatggctac gaaatgagca tcgccatgtc accctacatc tcataagagg
      121 atcgcttctg atgaatgcac tgaccgccgt acaaaataac gctgtcgatt cagaccagga
      181 ctatagcgga ttcactctca tcccgtcggc gcaatccccg cgtctgctgg aactcacctt
      241 caccgaacag acgaccaaac agtttctcga acaggttgcc gaatggcccg tgcaggcgct
      301 ggagtacaaa tcgtttctgc gttttcgggt aggtaaaatt cttgacgatc tgtgtgcgaa
      361 ccagctgcaa ccattgctgt tgaagaccct gctaaaccgt gctgaaggtg cgctgttgat
      421 caatgcggtg ggtgtcgatg atgtgaagca agcggatgag atggtgaagc tggcgacggc
      481 ggtggcgcat ctgattggcc gttccaattt cgatgcgatg agcggtcagt attacgcgcg
      541 ttttgtagtg aaaaatgtcg ataactcaga cagctatctg cgtcagccgc accgcgtaat
      601 ggagctgcac aacgacggca cctacgtcga agagatcact gactatgtgc tgatgatgaa
      661 aatcgacgag cagaacatgc agggtggaaa ttcgttgctg ctgcatctcg atgactggga
      721 acatctggac cactatttcc gccatccaat ggcgcgtcgc ccgatgcgct ttgccgcgcc
      781 gccgagcaaa aacgtcagca aagatgtttt ccatccagtg ttcgacgttg atcaacaggg
      841 tcgcccggtg atgcgctata tcgaccagtt cgtccagcca aaagacttcg aagaaggcgt
      901 gtggttgagc gagctttcgg acgccattga aatcagtaaa ggcattcttt ctgtacccgt
      961 tcccgttggc aaattcctgt tgattaacaa cctgttctgg ctgcacggtc gcgaccgctt
     1021 tactccgcac ccggatctgc gccgtgaact gatgcgtcag cgtggctatt tcgcttacgc
     1081 cactcaccac tatcagacgc atcagtaagc gcaaaggaat tgagcggatg tatgattttg
     1141 tgattattgg cggcggcatc atcggcatgt cgaccgccat gcaactgatt gatgtctatc
     1201 cggatgcccg cattgcgttg ctggaaaaag agtccggccc ggcctgtcat cagacgggcc
     1261 acaacagcgg cgtgatccat gccggggttt attacacgcc cggtagcctg aaggcgcagt
     1321 tttgcctggc gggaaaccgc gccactaaag ccttttgcga tcaaaacggc attcgctatg
     1381 acaactgcgg caagatgctg gtcgccacct ctgaactcga aatggaacgg atgcgcgcgt
     1441 tgtgggaacg cacggcggca aacggtatcg agcgcgagtg gttaaacgcc gatgaactgc
     1501 gcgagcgcga accgaatatc accgggcttg gcggtatttt tgtgccgtcc agcggcattg
     1561 tcagctaccg cgaagtcacg gcggcgatgg caaaaatttt ccaggccaga ggcggcgaga
     1621 ttatctataa cgccgaagtc agcggcctta gtgagcataa aagcggcgtg gtgatacgta
     1681 cccgtcaggg cagcgactat gaagcatcga cgctgattag ctgttccggg ctgatggctg
     1741 accggctggt gaaaatgctc ggcctcgaac cgggctttat tatctgcccg ttccgcggcg
     1801 agtatttccg cctggcgccg gagcataacc agattgttaa ccatctgatt taccccattc
     1861 ccgacccggc aatgccgttt ttgggcgtgc atctcacccg catgatcgac ggcagcgtca
     1921 ccgtcgggcc aaacgcggtg ctggctttca aacgcgaagg ctatcgcaag cgcgacttct
     1981 catttagcga cacgctggag attttgggct cgtcggggat tcgccgggtg ctgcaaaacc
     2041 atctacgctc aggactgggc gagatgaaaa actcgctgtg caaaagcggc tatctgcggc
     2101 tggtgcaaaa gtattgtccc cggctttcgt taagcgatct ccagccctgg cccgccggtg
     2161 tgcgggcgca ggcggtatcg ccggacggca agctgattga cgattttctg tttgtcacca
     2221 ccccgcgcac gatccacacc tgcaatgcgc cctccccggc agcgacatca gcaattccta
     2281 ttggtgcgca tattgtcagc aaggtacaaa cgctgttggc aagccagagt aaccccggac
     2341 gcacgctgcg agcggcacgt agtgtggatg ccttacacgc cgcgtttaat caataacctt
     2401 tgaaaacagg atgtagcgat gaaactgaac gacagtaact tattccgcca gcaggcgttg
     2461 attaacgggg aatggctgga cgccaacaat ggtgaagtca tcgacgtcac caatccggcg
     2521 aacggcgaca agctgggtag cgtgccgaaa atgggcgctg atgaaactcg cgccgctatc
     2581 gacgccgcca accgcgccct gcccgtctgg cgtgcgctca ccgccaaaga acgcgccaac
     2641 attctgcgca actggttcaa tttgatgatg gagcatcagg acgatttagc gcgcctgatg
     2701 accctcgaac agggtaaacc gctggctgaa gcgaaaggcg aaatcagcta cgccgcctct
     2761 tttattgagt ggtttgccga agaaggcaaa cgcatttatg gcgacaccat tcccggtcat
     2821 caggccgata agcgcctgat tgtgatcaag cagccgattg gcgttacagc ggccatcacg
     2881 ccgtggaact ttccggcggc aatgattacc cgcaaagccg gtccggcgct ggcggcaggc
     2941 tgcaccatgg tgctgaagcc cgccagtcag acgccgttct ctgcgctggc gctggcggag
     3001 ctggcgatcc gcgcgggcat tccggctggc gtgtttaacg tggtcaccgg ttcggcgggc
     3061 gcggtgggta atgaactgac cagcaacccg ctggtacgca aactgtcgtt taccggctcg
     3121 accgaaattg gccgccagtt aatggaacag tgcgcgaaag acatcaaaaa agtgtcgctg
     3181 gagctgggcg gcaacgcgcc gtttatcgtc tttgacgatg ccgatctcga taaagccgtg
     3241 gaaggcgcac tggcctcgaa attccgcaac gccgggcaaa cctgcgtctg cgccaaccgt
     3301 ttatacgtgc aggacggcgt gtatgaccgc tttgccgaaa aattgcagca ggcggtgagc
     3361 aaactgcata tcggcgacgg gctggataaa ggcgtcacca tcgggccgct gatcgatgaa
     3421 aaagcggtag caaaagtgga agagcatatt gccgatgcgc tggaaaaagg cgcgcgcgta
     3481 gtttgcggcg gtaaagcaca tgaacgcggc ggcaacttct tccagcccac cattctggtg
     3541 gacgtgccgg ccaacgccaa agtgtcgaaa gaagagacct tcggccccct cgccccgctg
     3601 ttccgtttta aagatgaagc cgatgtgatc gcgcaagcca atgacaccga gtttggcctt
     3661 gccgcctatt tctatgcccg tgatttaagt cgcgtcttcc gcgtgggcga ggcgctggag
     3721 tacggcatcg tcggcatcaa taccgggatt atttccaatg aagtggcccc gttcggcggc
     3781 attaaagcct cgggtctggg tcgtgaaggt tcgaagtatg gcatcgaaga ttacttagaa
     3841 atcaaatata tgtgcatcgg tctttaactg gagaacgcga atgagcagca ataaagagtt
     3901 aatgcagcgc cgcagtcagg caattcctcg tggcgttggg caaattcacc ccattttcgc
     3961 tgatcgcgcg gaaaactgcc gggtgtggga cgttgaaggc cgtgagtatc ttgatttcgc
     4021 gggcggcatt gcggtgctca ataccgggca cctgcatccg aaagtggttg ccgcggtgga
     4081 agcgcagttg aaaaaactgt cgcacacctg cttccaggtg ctggcttacg agccgtatct
     4141 ggagctgtgc gagattatga atcagaaggt gccgggcgat tttgccaaga aaacgctgct
     4201 ggttacgacc ggttccgaag cggtggaaaa cgcggtgaaa atcgcccgcg ctgccaccaa
     4261 acgtagcggc accatcgctt ttagcggcgc gtatcacggg cgcacgcatt acacgctggc
     4321 gctgaccggc aaggtgaatc cgtactctgc gggcatgggc ctgatgccag ggcacgttta
     4381 tcgcgcgctt tatccttgcc cactgcacgg catcagtgaa gatgatgcta tcgccagtat
     4441 ccaccgaatt tttaaaaatg atgctgcgcc ggaagatatc gccgccatcg tgattgagcc
     4501 ggttcagggc gaaggcggtt tctacgccgc gacgcctgcg tttatgcagc gtttacgcgc
     4561 gctgtgtgac gagcacggga tcatgctgat tgccgatgaa gtgcagagcg gcgcggggcg
     4621 taccggcacg ctgtttgcga tggagcaaat gggcgtggca ccagatctca ccacctttgc
     4681 gaaatcgatc gcaggcggct tcccactggc gggcgtcacc gggcgcgccg aagtgatgga
     4741 tgccgtcgct ccaggcgggc tgggtggcac ctatgccggt aatccgattg cctgcgtggc
     4801 ggcgctggaa gtgttgaagg tgttcgagca ggaaaatctg ctgcagaaag ccaacgatct
     4861 ggggcagaag ttgaaagatg gattgttggc gatcgccgaa aaacaccctg agatcggcga
     4921 cgtacgcggg ctgggggcga tgatcgccat cgagctgttt gaagacggcg atcacaacaa
     4981 gccggacgcc aaactcaccg ccgagatcgt ggctcgcgcc cgcgataaag gcctgattct
     5041 tctctcctgc ggcccgtatt acaacgtgct gcgcatcctt gtaccgctca ccattgaaga
     5101 cgctcagatc cgtcagggtc tggagatcat cagccagtgt tttgctgagg caaagcagta
     5161 gcgccgctcc tatgccggag gcgacgctgc gcgtcttgtc cggcctacgg ggatccaggt
     5221 cggataaggc gtttacgccg catccgacaa tctgtacgtg aacaggaata aatctatgtt
     5281 ggccgggtaa ggcggagccg ctctccggca aaaagaatca ataacaatta tacgcgtgac
     5341 ccgacgcggg aaatgtcggg gcgctctccc aagtgacaca ctttcgagag gattcaggat
     5401 ggggcaatca tcgcaaccac atgagttagg cggcgggctg aagtcacgcc atgtcaccat
     5461 gttgtctatt gccggtgtta tcggcgcaag tctgtttgtc ggttccagcg tcgccatcgc
     5521 cgaagcgggc ccggcggtat tactggccta tctgttcgcc gggctactgg tggttatgat
     5581 tatgcggatg ttggcggaaa tggcggttgc cacgcccgat accggttcgt tttccaccta
     5641 tgccgataaa gctattggtc gttgggcggg ttataccatc ggctggttgt actggtggtt
     5701 ttgggtactg gttatcccgc tggaagccaa catcgccgcc atgatcctgc actcatgggt
     5761 tccaggcatt cccatctggt tattttccct cgtcattacc cttgccttaa ctggcagtaa
     5821 tttattaagc gttaaaaact acggcgaatt tgagttctgg ctggcgctgt gcaaagtcat
     5881 cgctatcctg gcctttattt tccttggtgc agtcgcaatt agcggttttt acccgtatgc
     5941 cgacgtgagc gggatctcaa gattgtggga tagcggcggc tttatgccca acggcttcgg
     6001 tgcggtatta agcgcgatgt tgatcaccat gttctcgttt atgggcgcag aaattgtcac
     6061 cattgccgcc gcggaatccg acacgccgga aaaacatatt gtccgcgcta ccaactcggt
     6121 tatctggcgt atttctatct tctatttgtg ctctattttt gtcgtagtgg cgttaatacc
     6181 gtggaatatg ccggggctga aagccgttgg atcttatcgc tcggttctgg aattgctcaa
     6241 tataccccat gcgaaattaa tcatggactg cgtgatatta ctttccgtaa ccagttgcct
     6301 gaactcggcg ctgtataccg cgtcaaggat gctctactcc ttaagccgtc gcggtgatgc
     6361 gcccgcggta atgggcaaaa tcaaccgcag taaaaccccg tatgtggcgg tgttactctc
     6421 caccggagcg gcatttttaa cggtcgtggt gaactattac gcacctgcga aagtgtttaa
     6481 attcctgatc gacagctccg gtgctatcgc cctgctggtt tatttagtca tcgccgtttc
     6541 acagttgcgg atgcgtaaaa ttctgcgagc agaaggaagc gaaattcgct tgcgcatgtg
     6601 gctttacccg tggctcacct ggctggtaat cggctttatt acctttgtgt tggtggtgat
     6661 gttattccgc ccggcgcaac agttagaagt gatctctacc ggcttattag cgatagggat
     6721 tatctgtacc gtgccgatta tggcgcgctg gaaaaagctg gtattgtggc aaaaaacacc
     6781 cgttcataat acgcgctgat aatgatcagg agtcacacca tgaccattac gtctctggat
     6841 ggctatcgct ggctgaagaa cgatattatt cgcggtaatt ttcaaccgga tgaaaaatta
     6901 cgaatgagtt tgctgacatc gcgttatgca cttggcgttg ggccgttacg ggaagctctt
     6961 tcgcaactgg tggcggaacg gctggtcacg gtggtgaatc aaaaagggta tcgggtggcg
     7021 tctatgtcag agcaggagct gctcgatatt ttcgacgccc gcgccaatat ggaagcgatg
     7081 ttagtgagtc tggcgattgc ccgcggtggc gatgagtggg aggcagacgt tctcgcaaaa
     7141 gcgcatctgc tgagtaagct tgaggcctgt gacgccagcg agaaaatgct tgatgagtgg
     7201 gatctgcgtc atcaggcgtt tcatactgca attgtggcgg gctgtggttc tcactatttg
     7261 ctgcaaatgc gtgaacggtt gtttgatctg gcggcgcgtt atcgatttat ctggctgcgg
     7321 cgaacggtgc tttcggtgga aatgctggag gataaacacg atcagcacca gaccctgact
     7381 gcggcggtac tggcgcgaga taccgcgcgc gccagtgagt taatgcgcca gcatttactg
     7441 acgccaattc ccattatcca gcaggcgatg gctggcaatt aactactctt ccggaatacg
     7501 caacacttgc cccggataaa ttttatccgg gctttttagc atcggtttat tcgcttcgaa
     7561 gattttattg tacagattag cgttaccgta gacctgtttg gaaatggcac tcagagtgtc
     7621 gccagactta acggtataaa actggctggc agtggctggt gtcgccgttt tcacctgatc
     7681 atcaacactg gcaataccgg aaatattccc caccgcaaca aggattttct ccttcgcctc
     7741 ctgactcagg ccgtcaccag tgaccgtcgc tttgccatcg gcaatttgaa tgttcacttt
     7801 atcggcatca ggtataccgg ttttgctcag atgctcctgc accttcttcg cctgatcatc
     7861 tttatcgtgc tgacctgtaa ccgcgtccca gagtttttct ccggcatctt tcacaaaatt
     7921 gaacagaccc atagctacct cattcgttaa cggaaacact cagaaagtgt agtagaggaa
     7981 cgcggcgcgt taacgccgcg gggatgagca caggtttaat cgcgggtttg cacccagaac
     8041 gcgtgaatca aaccggggat atacccaagc agtgtcaaca gaatattaat aatgaacgcc
     8101 caaccgaacc ctttaccgag cagcacgccg agcggcggca gaatgatggt gatgacgat
//
LOCUS       DBFIFI010000191         8141 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.191, whole genome shotgun sequence.
ACCESSION   DBFIFI010000191 DBFIFI010000000
VERSION     DBFIFI010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8141
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.191"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            425..1249
                     /gene="gadX"
                     /locus_tag="N7B39_003935"
     CDS             425..1249
                     /gene="gadX"
                     /locus_tag="N7B39_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HGU2423828.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKVSSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDDHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDCSAEGIRL"
     gene            1617..2345
                     /gene="gadW"
                     /locus_tag="N7B39_003936"
     CDS             1617..2345
                     /gene="gadW"
                     /locus_tag="N7B39_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HGU2423829.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2708..5821)
                     /gene="mdtF"
                     /locus_tag="N7B39_003937"
     CDS             complement(2708..5821)
                     /gene="mdtF"
                     /locus_tag="N7B39_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HGU2423830.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVIYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPNQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5846..6955)
                     /gene="mdtE"
                     /locus_tag="N7B39_003938"
     CDS             complement(5846..6955)
                     /gene="mdtE"
                     /locus_tag="N7B39_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HGU2423831.1"
                     /translation="MLTACDDKSAENTAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVKLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            7063..7341
                     /locus_tag="N7B39_003939"
     CDS             7063..7341
                     /locus_tag="N7B39_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2423832.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(7342..7869)
                     /gene="gadE"
                     /locus_tag="N7B39_003940"
     CDS             complement(7342..7869)
                     /gene="gadE"
                     /locus_tag="N7B39_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HGU2423833.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
BASE COUNT         2123 a         1892 c         1906 g         2220 t
ORIGIN      
        1 gatcacccga aactgcaggg tattgcccag cagaacagct ttaaacacac ctgataacat
       61 aacgttgtaa aaaccgaatg cccagccttt aaaaaaaaca gctgggcatt cggttgctta
      121 ttaatcgcaa taatatattg gctgtctatt catcgtgttg ataagatata tgttatatga
      181 atgtttatta aatgtagcga ttacctttaa tgtcacgaca agggattatt tgcttactat
      241 taatttccct gcgttcttaa acacgttttt gtattataaa tccagtcatc ctgcccgtag
      301 aacatcacac attatcatcc tgttctcccg cttcgtttaa atttatttat caatcaattt
      361 gacttaagag ggcggcgtgc tacattaata aacagtaata tgtttatgta atattaagtc
      421 aactatgcaa tcattacatg ggaattgtct aattgcgtac gcaagacata aatatattct
      481 caccatggtt aatggtgaat atcgctattt taatggcggt gacctggttt ttgcggatgc
      541 aagccaaatt cgagtagata agtgtgttga aaattttgta ttagtatcaa gggatacgct
      601 ttcattattt ctcccgatgc tcaaggagga ggcattaaat cttcatgcac ataaaaaagt
      661 ttcttcatta ctcgttcatc actgtagcag agatattcct gtttttcagg aagttgcgca
      721 actatcgcag aataagaatc ttcgctatgc agaaatgcta cgtaaaagag cattaatctt
      781 tgcgttgtta tctgtttttc ttgaggatga tcactttata ccgctgcttc tgaacgtttt
      841 acaaccgaac atgcgaacac gagtttgtac ggttatcaat aataatatcg ctcatgagtg
      901 gacactagcc cgaatcgcca gcgagctgtt gatgagtcca agtctgttaa agaaaaaatt
      961 acgcgaagaa gagacatcat attcacagtt gcttactgag tgtagaatgc aacgtgcttt
     1021 gcaacttatt gttatacatg gtttttcaat taagcgagtc gcagtgtcct gtggatatca
     1081 cagcgtgtcg tatttcattt acgtctttcg aaattattat gggatgacgc ccacagagta
     1141 tcaggagcga tcggcgcagg gattgccgaa ccgtgactcg gcggcaagta ttgttgcgca
     1201 agggaatttt tacggcactg actgttctgc ggaaggaata agattataga gttttactca
     1261 gacataaaaa aaacccggca taggggaccg ggaagaggat agtctgccgt ctccagacta
     1321 ataaaccgtt ataacactcc ctgttggcac gggaaacttt gtgctctcag taagttaaat
     1381 ataactttta ctggaaataa gatcagccat ttttttataa acataagcta tacgctgtgc
     1441 gaaaatatat tcgtgttgca tttacttatt atcaattaac tgttatgcaa aactactttg
     1501 tggataaatt ttggtcctac caaatttggc agtttttgcg ctaagaaaca gtctggcatc
     1561 atttcattag tatactgaaa ttgaaataat cgcagtatga aatataaggg ataatcatga
     1621 ctcatgtctg ctcggtgatc ctcattcgtc gttcattcga tatttatcat gaacagcaaa
     1681 aaatatcgct gcataacgag agtatcctgc tgctggagaa aaatttggca gacgattttg
     1741 cgttttgttc accggatacg cgacgactgg atatcgatga gctgacagtt tgccattact
     1801 tacaaaatat tcgtcagcta ccacgcaatt tagggttaca tagcaaagac cgtctgttaa
     1861 ttaaccagtc accccccatg ccgctggtga cggcgatttt tgatagcttc aatgaatccg
     1921 gggtaaattc accgatactg agcaatatgc tctacctttc ctgtttatcg atgttttctc
     1981 ataagaaaga actgatcccc ttacttttca atagcatcag cactgtttca ggaaaagttg
     2041 aacgccttat tagctttgat atcgccaaac gttggtatct gcgcgatatc gcggaaagaa
     2101 tgtataccag cgagagtctc atcaaaaaaa agttgcagga tgaaaacacc tgtttcagta
     2161 aaatattact cgcctccagg atgtcgatgg ccagacgatt actcgagtta cgtcaaattc
     2221 ctctgcatac tattgcggaa aaatgtggct atagcagtac gtcgtacttt ataaacacat
     2281 ttcgacaata ttatggtgta acgccacatc agtttgcgca acattcgcca ggtacctttt
     2341 cctgacatat tttgcatttg aatattggtc aggatctcac acctgcttca tgtgaaactc
     2401 ttccctgatg atttctgccg ggctaccggc tagttctctt tcgcagtttg ccaggcggta
     2461 taggtatttt caggaggaaa agactcggca tgtttgggat tattaagctg acaattcata
     2521 cgattacggg aatgtgggtc agtattgtgc tgttcaaatt gatgacgaat ggctggagtg
     2581 gtttctactt tcagtgctgt gtactaagcc tggtattcct gacggttagc tggttgttga
     2641 gtggtgaatg gttagcagga aagagtaagg ctgaaccttc acgttcaacc ttactctcat
     2701 ttacacgtta cgctttttta aagcgggcaa agagatgttc cactacaacg aaaaagaccg
     2761 gaacaaagta aattgccagc actgttgcgg caaacatccc gcccatcacg ccggtaccta
     2821 ccgcgttttg cgcgccagaa ccggcaccat gactgataac cagcggcagc acgccgagaa
     2881 taaaggccag agaggtcatc aggattgggc gtaaacgcat ccgcgccgct tcgatgattg
     2941 cctctatcgg cgttttccct tctttctgca tcatctcaac ggcaaattcg acaatcagga
     3001 tggcgttctt ggcggaaagc ccgatggtgg tcagcaaacc aacctggaag tagacgtcat
     3061 tacttaagcc gcgcagatcg gtggccagta atgcgccaac gacgcctaac ggaacaacca
     3121 acatcaccga gaacggaatt gaccagctct cataaagtgc ggcgagggcg aggaacacca
     3181 cgaccagtga aatcgcatac agcgcaggag cctgatttga ggataacgct tcctgatacg
     3241 acagtccggt ccatgagtag ccgacgcctg ccggaagttt agcgaccagg tctgccataa
     3301 atttcatggc gtcaccggta cttttcccgg ccgccgcttc acctaaaatc tccattgacg
     3361 ggatgccgtt gtagcgttcc agtcgcggtg aaccataggt ccattcggta gacgagtagg
     3421 cagaaagcgg tgccatcgtg ccagaggcgt tgcgtacata ccattggttg atgttatccg
     3481 gcaacatacg gaacggcgtg cctgcctgga catacacttt tttcacccgc ccctggttga
     3541 ggaagtcgtt cacgtagctg ctgccgaagg cggtggaaat tgtctggttg atatcagaca
     3601 gcgccacacc cattgcctca gctttcgcgg cgttgacgtt cactttgaac atcggcgtat
     3661 cttccaggcc gttcgggcgt accccggtga cctgattcgg tgattgcgct gccagtgata
     3721 acagctcgtt tcgtgcctgg gttagttttt cgtgccccag gttaccgttg tccagcagtt
     3781 ccatatcaaa acctgacgcg gtacccagtt cagccaccgc gggtaagttg aacgggaaga
     3841 cgacggcttt attgatactg cttagcgcaa tcattgcccg ctgaatgatc gcggtaaccg
     3901 agttttcctc accgacacgt tcagaccacg gcttgagact gataaacgcc agaccgttgt
     3961 tttgcccctg gccgctgaag ccaaagccgc caacggtaaa caccgactga acattatctt
     4021 tctcttttgt aagataataa tccgtcacct gttgcagcac tttcgtggtg ttaaccatgg
     4081 tggcaccgga aggtaactgc gcggtggtca taaatacccc ctgatcctct tctggtaaga
     4141 aagaggtcgg cgtgcgcagg aacagcaccg ccatcccggc gcaaatcagc aggtagatca
     4201 ccatgtagcg accggtacaa cgcaacagtg agcgggtgct atcggtatag tgctgagttg
     4261 atttttcaaa cagcgtgttg aagcgtgcga acagggcatt aggtttgtga ccgccttccg
     4321 gcgcggcttt cagaatggtg gcgcacaggg caggggtcag gctcattgcc acaaatactg
     4381 aaagcagcat ggaggagatc agcgtgatgg agaactggcg gtagatctcc ccggttgcac
     4441 cgctcataaa ggccatcggc ataaacactg cggaaagaac aacggcaata ccgaccagcg
     4501 cacgttggat ctgccccatc gatttatgcg tcgcttcctt cggtggtagc ttatcttccg
     4561 caatgacacg ctcgacgttc tccaccacca cgatggcgtc atccaccagt aaccctatcg
     4621 ccagcaccat cccgaacata gtcaacgtgt tgatggtgaa accgaccgcc gacaagatcg
     4681 caaacgtccc gagaataacc accggtacgg caatcgtcgg gatgattgtg gcacggaaat
     4741 tctgcaaaaa cagatacatg accaggaaga cgaggatgat agcctcaacc agtgttttga
     4801 aaacttcctg aatagaaatt tcgataaacg gcgtggtgtc gtaaggataa accgtcttca
     4861 gacttgccgg gaaataggct gataagcggt tcagttcctc tttgactgcc cgcgaggtat
     4921 ccagggcgtt tgctccggta gccagtttga tggcgatccc ggcagcaggt ttgccgttat
     4981 agcgcgccac ggtggaataa tcttccgccc caagttcgac gcgggcgaca tcacgcagca
     5041 gcacttgcga accatcttgc tgaactttca acaggatttt gccaaattct tccggcgttt
     5101 gcagacgcgt ctgcacaatg atcgaggcgt ttagttgctg gtctgccgcc tgtggcatgc
     5161 cacccagttg accaccagaa atctggttgt tttgcacctt aatctgggaa ataacatcgg
     5221 aaggtaccag attgtattta ttgagttttt gcgggtccag ccagatacgc atggcgtact
     5281 cggaaccaaa gagctgtacg ctaccaacgc ccgcggtacg gcttagcggg tctttgatat
     5341 tagacgctac atagtccgcg atatcgtact ggttgaggct gccgttatca gaaataaacg
     5401 ccgctaccat caggatatta ctgctcgatt tatcgacgct aatcccctgc tgctgcactg
     5461 cttcaggtaa tgaaggcata gcgagttgca gtttattttg cacctgaacc tgtgcgatat
     5521 caggagatgt cccagtctcg aaggtcagag tgatagaggc attacccgcc gcatcactgg
     5581 ttgaagacat gtacatcagg ccatcaagcc cattcatatt ttgctcaatc acctgagtga
     5641 ccgagtcttc taccgtttgc gcatcggcac ccggataggt agcgctgacg gtaatggtcg
     5701 gtggcgcaat ctgcggatac tgcgcaaccg gtaagttcat gatcgccaga ccacctgcaa
     5761 gcatcataat aatggcaagt acccaggcaa aaaccgggcg atcaataaaa tagttagcca
     5821 tgaaagtccc cttaagcctg caacgttatt gtttcgattc ggtgctggcg ttttcctggc
     5881 tggaggaaat tgctcgtgct ttgatacccg gacgaatgcg ttgcaaaccg gaaacgatca
     5941 cccgatcgcc agcctgcaag ccagaggtga caacccactg gtcgccgatg gctttgctgg
     6001 cttcaatttc gcgtagcttc acgacatcgt ctttatccag aatgagcgcc gttgctttac
     6061 cttgggcgtt gtgggtgacg ccttcctgcg gcaccagtaa tacattctgg cggctacctt
     6121 catccactaa tgccgtgaca tacatgccag gcagcaagtc accatttggg ttggggaaaa
     6181 tcgcccgtaa cgtcacggag cccgtggttt catcaaccgt cgggtcggag aatttcagcg
     6241 tgccggtctg gctgtaacgt ttaccatttt ccagattgag ctgtactggc gtactgccct
     6301 gaacctgttt gatttgccca ctggcgacct cttctttcat gcgtaagaaa tcttgcaccg
     6361 actgcgtgag atcgacataa atcgggtcca gacgttgtac ggtaaccagc gaatctgcct
     6421 gattagcggt aacgagtgcg ccgacggtca ccgacgattt cccgctgacg cccgtaatcg
     6481 gcgaggtgac attcgcgtat tgcagattga tcgtcgcctg ttcaacagcc gctttggcga
     6541 cggtgacatt ggcttctgct tcattcaact gggtgcgcgc ggtgtcgtaa tcctgacggc
     6601 taacgtagtt ggtcttcagc aacgatgcct ggcggttaaa ggtgatgcgg gcattgctgg
     6661 cggtagagag cgctttcgcc agcgagcctt tggcggagtt tagctcggcc tgtaaaggtg
     6721 caggatcaat ctgatacagc gaatcgccct ggttcacttt atcgccttcg ataaagttgc
     6781 gtttaatgat aataccgccc acctggggac gtatctcggc aacttcataa ggaacggttc
     6841 taccgggcaa ttcgctcaac acattgaccg acccagggga gagtgtgacg acaccgacct
     6901 caggcgtcat ggcggcggtg ttttccgccg atttgtcatc gcaggcggtg agcatcgcgc
     6961 cgcagaataa caacggtatt aacagctttc ttcttctgtt cattttagtc cctgaaaatt
     7021 cttgagaaat aacggttctt taaattgcgc gcgaggagag atatgaggga tttacagacc
     7081 agcggcatag tggggttgtc agccagtaag gtgggataca ggcatagtga tcgacatggt
     7141 gaggtcaacg acaaaaaaaa tgttgccgtt ctgccaacag ttcctgccag cattcgggca
     7201 acgaagggtt ctactggtgg atacacatac caggggaata aagtcgtttt agcctctcct
     7261 ttagtaatca ccgggggtaa tgaaatcagc atctgcatcc ctcgtcatgc cagccatcaa
     7321 tttcagttgc ttatgtcctg actaaaaata agatgtgata cccagggtga cgatgtcgct
     7381 catacgtctt agtttcagct tgtacataat gttgaatttc tgaatcttta atgtttgttg
     7441 cgaaatattg agcgtcgacg tgatattgct ttcattttta tttttcagaa tcagttgcat
     7501 aattttgaac tgattatgtg aaaaacataa ttgtgaattc ttatggggca agtgtttacc
     7561 ataagtaatc tcaagaagat ttttgatact ttctttgcgg ctaacaaaat aaataggcgc
     7621 tttagctttt agtttactga tgtgataagg tgccaaaaca ataattctct cggcatctaa
     7681 tttctccaga aatttaatcg cttcttcatc aaggatatga ttgtgatagg tatcaatgat
     7741 aaccttgaag ggtttattgc ctactttttt gatctctgac agggaattga tttttatcat
     7801 ttcgtgatta tctttcaact gccaaaagcc ctgtaaaaga aaagaatctt tcgtcatgag
     7861 aaaaatcata acttgctcct tagccgttat cgtttcgaac atgtcatcct tttcattatt
     7921 tacatccttg tccgaatcgt tgttcaatat agtaaacgcc tataagaact ttgcccttcg
     7981 tgaactaata cgtctattaa cactaatcaa tacttcagat ttatccatca cgcactccat
     8041 atattgatcc taagtaagat tcctggttgt tatcagcttg taaatagtaa acaaaaagtt
     8101 aacgtttgcg gattactgcc caagaataag ttatcaataa c
//
LOCUS       DBFIFI010000192         8137 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.192, whole genome shotgun sequence.
ACCESSION   DBFIFI010000192 DBFIFI010000000
VERSION     DBFIFI010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8137
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.192"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            1..498
                     /gene="ftnA"
                     /locus_tag="N7B39_003941"
     CDS             1..498
                     /gene="ftnA"
                     /locus_tag="N7B39_003941"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HGU2423834.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(535..774)
                     /gene="yecH"
                     /locus_tag="N7B39_003942"
     CDS             complement(535..774)
                     /gene="yecH"
                     /locus_tag="N7B39_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HGU2423835.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            966..2177
                     /gene="tyrP"
                     /locus_tag="N7B39_003943"
     CDS             966..2177
                     /gene="tyrP"
                     /locus_tag="N7B39_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HGU2423836.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(2239..2904)
                     /gene="yecA"
                     /locus_tag="N7B39_003944"
     CDS             complement(2239..2904)
                     /gene="yecA"
                     /locus_tag="N7B39_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HGU2423837.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPAQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(3100..3186)
                     /locus_tag="N7B39_003945"
     tRNA            complement(3100..3186)
                     /locus_tag="N7B39_003945"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3150..3152),aa:Leu,seq:taa)
     gene            complement(3199..3272)
                     /locus_tag="N7B39_003946"
     tRNA            complement(3199..3272)
                     /locus_tag="N7B39_003946"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3238..3240),aa:Cys,seq:gca)
     gene            complement(3326..3401)
                     /locus_tag="N7B39_003947"
     tRNA            complement(3326..3401)
                     /locus_tag="N7B39_003947"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3366..3368),aa:Gly,seq:gcc)
     gene            complement(3553..4101)
                     /gene="pgsA"
                     /locus_tag="N7B39_003948"
     CDS             complement(3553..4101)
                     /gene="pgsA"
                     /locus_tag="N7B39_003948"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HGU2423838.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(4158..5990)
                     /gene="uvrC"
                     /locus_tag="N7B39_003949"
     CDS             complement(4158..5990)
                     /gene="uvrC"
                     /locus_tag="N7B39_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HGU2423839.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(5987..6643)
                     /gene="uvrY"
                     /locus_tag="N7B39_003950"
     CDS             complement(5987..6643)
                     /gene="uvrY"
                     /locus_tag="N7B39_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HGU2423840.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     ANAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            6785..6901
                     /locus_tag="N7B39_003951"
     CDS             6785..6901
                     /locus_tag="N7B39_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU2423841.1"
                     /translation="MVTPVTNGLPDRFTVSPATKCDKNYLYSAKMKISPLNM"
     gene            6939..7115
                     /gene="yecU"
                     /locus_tag="N7B39_003952"
     CDS             6939..7115
                     /gene="yecU"
                     /locus_tag="N7B39_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HGU2423842.1"
                     /translation="MIKIFIGHYINAFNSTVDGTLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            7102..7326
                     /gene="yecF"
                     /locus_tag="N7B39_003953"
     CDS             7102..7326
                     /gene="yecF"
                     /locus_tag="N7B39_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HGU2423843.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEQQLALIEDEPQAAVFSKTVKQIKQAYRQ"
     gene            complement(7394..8116)
                     /gene="sdiA"
                     /locus_tag="N7B39_003954"
     CDS             complement(7394..8116)
                     /gene="sdiA"
                     /locus_tag="N7B39_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HGU2423844.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMETAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFNE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSAREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILRWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
BASE COUNT         2011 a         2091 c         1910 g         2125 t
ORIGIN      
        1 atgctgaaac cagaaatgat tgaaaaactt aatgagcaga tgaacctgga actgtactct
       61 tcactgcttt atcagcaaat gagcgcctgg tgcagctatc ataccttcga aggtgctgcc
      121 gcgttcctgc gccgtcacgc ccaggaagag atgacgcata tgcagcgtct gtttgattac
      181 ctgactgata ccggcaattt accgcgtatt aataccgttg aatctccgtt tgctgaatat
      241 tcctcacttg atgaattatt ccaggaaacc tataaacacg aacaattaat cacccagaaa
      301 attaacgaac tggctcatgc tgcaatgacc aatcaggact acccaacatt taatttcctg
      361 cagtggtatg tttctgagca gcatgaagaa gagaaactgt tcaaatcgat tattgataaa
      421 ttaagcctgg caggcaaaag cggcgaaggt ctgtatttta tcgacaaaga actctctacc
      481 ctcgacacac aaaactaatg ctctccggcg gcaggtttct gccgccattt caaatcagtg
      541 acggcaaatc ttactctgat cggtcgaaaa accttcttct gaaggtataa atttgccttt
      601 tgctgccaga aacgctacca gctctcccgc tgtcatccct tctgccgagc aagtgtgaaa
      661 acgtgcctgt tcaccaaaac gcgctttaat cgcagcttcc agactggcat gcgtatattg
      721 ctcgcctgat tcaatcatca tatttaacac ttcatgaccg tgaatagagt ccatcgttcc
      781 tcctcaaaaa aaagcctagc gtagcgattg ccgcttatga agactttgcg ccagcgcagg
      841 actgaatgct ttttattgta catttatgtt tacaccatat gtaacgtcgg tttgacgaag
      901 cagccgttat gccttaacct gcgccgcaga tatcactcat aaagatcgtc aggacagaag
      961 aaagcgtgaa aaacagaacc ctgggaagtg tttttatcgt ggcgggaacc acaattggcg
     1021 caggcatgct ggcaatgccg ctggctgcgg ccggtgttgg ttttagcgtt acgttaatct
     1081 tgttgattgg gctttgggcg ttgatgtgct acacggcgct attactgctg gaggtgtacc
     1141 agcatgttcc ggcagatacc ggtctgggca cgctggcaaa acgctatctg ggacgctacg
     1201 gtcaatggct gacgggcttc agtatgatgt tcttaatgta tgctctgact gcggcataca
     1261 tcagcggtgc cggtgaattg ttggcctcca gcatcagcga ctggacaggt atttcaatgt
     1321 cggcaaccgc tggcgtgttg ttgttcactt ttgttgccgg tggcgtggtt tgtgtcggga
     1381 catcgctggt cgatttattt aaccgttttc tgttcagcgc caaaattatt tttctggtgg
     1441 tgatgctggt attgctgcta ccgcatattc acaaagtgaa tcttttaacc ctgccgttgc
     1501 aacaggggct ggcactgtct gcaatcccgg tgatttttac gtcgtttggt tttcacggta
     1561 gcgtgccgag tattgtcagc tatatggatg gcaacattcg taagctacgc tgggtgttta
     1621 taaccggtag tgcgatcccc ctggtggcat atattttctg gcaggtggcg acgcttggca
     1681 gcattgattc aacaaccttt atgggattgc tggctaatca tgccggatta aacgggctgt
     1741 tacaggcgtt acgcgaaatg gtggcctctc cgcatgttga gctggcagtg catttatttg
     1801 ctgatttagc ccttgccacg tcatttctcg gcgttgcgtt aggcttattt gattatctgg
     1861 cagatttgtt tcagcgttca aataccgttg gtggacgatt gcaaactgga gcaattacct
     1921 ttctgccacc gttggcgttt gcactgtttt atccacgagg atttgtgatg gcgctgggtt
     1981 acgccggtgt ggcgctggcg gtactggcat tgattatccc ttcgctgttg acctggcaaa
     2041 gcagaaagca caatcctcaa gcgggttacc gggtcaaagg tggtcgtccg gcgctggtgg
     2101 tggtgtttct ctgtggtatt gctgtgattg gcgtgcaatt tttgattgcg gcagggttgt
     2161 taccagaagt ggggtgatta gatagcctca aattccttat tgggtgccag aattaacgct
     2221 gacacccaat ttgacttctt aatgcaggca gcactgctta aattttttac cactaccgca
     2281 cgggcaagga tcgttacgcc ccggtttctc ttctgctttg atcggttgct gaacagcttt
     2341 ttcctgcgga tgcgccatcc agtacgcatg tagatcaagc gccgccagtc gaatggcatc
     2401 tacgctctct tcaaacgctt ctggcgacat cttctctacc cgctcgaagt tttcctcagt
     2461 accgtgcagc gctatcgcct ccagcgctgg ttttaacgaa tcgggcaatg ttgaccagtc
     2521 agaaagtgcc acgccccgca tatagccaaa gcaccactcc tcaacaatcg tcagctcgct
     2581 gccatcaact tctcgcaagc cgaataacgg ctcaaactgc tccgggaatt cgttcagacg
     2641 ctctgcggta tcggccatat gttgaaaagc caggttcata aagcgcgtca tctctttctc
     2701 tgacgcccag cgcggcacat agtcagcccc accccacacg gcaaccagcc actgtgccgg
     2761 ttcaatctct tgcggagaac tcaacaccgc cgtcaataaa ccgtccagct ccgccacatc
     2821 aaggatggcg tggtcagtgt tgtatttggt cagaatatcg tccagccatt ccaactcact
     2881 ttcgtttaac ggtcccgttt tcatacgctt ttccttgtgg atctcaactc gccagcacct
     2941 atcttacatg ccggtccgta tcagagatac tttttgagtg gctttgccgg tgattaaaaa
     3001 tcaaggaggg tgtaacgaca agttgcaggc acaaaaaaac cacccgaagg tggtttcacg
     3061 acactgctta ttgctttgat tttattctta tctttcccat ggtacccgga gcgggacttg
     3121 aacccgcaca gcgcgaacgc cgagggattt taaatccctt gtgtctaccg attccaccat
     3181 ccgggctcgg gaagaaagtg gaggcgcgtt ccggagtcga accggactag acggatttgc
     3241 aatccgctac ataaccgctt tgttaacgcg ccaaattctt caggcctttc agccagacat
     3301 ccgctgacgc cgatgtcttt taaactggag cgggaaacga gactcgaact cgcgaccccg
     3361 accttggcaa ggtcgtgctc taccaactga gctattcccg cattcatcaa gcaatcagtt
     3421 aatcacttga ttttattatc gtctggcaat cagtgccgcc gttcgatgcg ttgcattcta
     3481 cttacctggc gcgatgagtc aacgatattt ttcaccactt ttgatcgttt gctgaaaatt
     3541 gcgccgaaac gatcactgat caagcaaatc tgcacgcgca gcgctcaaat attgcaacat
     3601 tgaccacaga gtcagtaccg cagccacaaa gaaaagtgca ataccggcgt actcaaccca
     3661 aatgttcgga cgccacagca gccatgccaa cgccaccatc tgggcagtgg ttttcacttt
     3721 cccaatccag gagacagcca cgctactgcg tttacccaac tccgccatcc attcgcgtag
     3781 cgcagaaata ataatttcac gggcgatcat cgttgccgcc ggtaaggtca cccaccagct
     3841 gtggtaatgc tcggttacca gcaccatggc gatagccacg agaactttat ctgctacagg
     3901 gtcaaggaaa gcaccaaacc gggtactctg gttccagcgg cgtgccagaa aaccatcgaa
     3961 ccagtcagtc accgccgcga cgcagaaaat gagcgcggcg gcaaacggcg accaagtgac
     4021 aggcagataa aagaccaata caaagaatgg gataaggatg acacggaaca gtgtaagcaa
     4081 cgtagggata ttaaattgca taatgacggg taactatctg ttgtcagtaa gattacccct
     4141 atgttgctac agagacatca atgtttcaac gaccagaaga tcttttctgc cagaccttgc
     4201 gaaatacccg gcacttttgc aatttcctcg acgctggcgt tacgtaaacc ttgcaaaccg
     4261 cccatatatt tcaacaacat ttgccggcgt tttggcccga cgccttcaat ggtttccagg
     4321 gaactggtat ttttgacctt cgcccgtttt ttacggtgcc cgccaatcgc gtgatcgtgt
     4381 gattcatcgc gaatatgctg gataacatgc agcgcgggag aatctggcgg caaactgaat
     4441 ccctcacctt ccggctcaaa gaacagcgtt tccagcccag ccttacgatc tgctcctttg
     4501 gcaacaccaa gtagcagcgg atgattttta tcccatgaga catccagttc ggcgaagaca
     4561 tttttcgcct gcgcaagctg gcctttgccg ccgtcgataa gtatcacatc cgggatctta
     4621 ctgtcgtcga tggctttacc ataacgccga cgcagcacct gattcatcgc cgcataatca
     4681 tcgcccggcg tgatgccagt aatgttatag cgccgatact ccgcacgcag cgggccgtta
     4741 gcatcaaaca ccacacagga agcgacggtt tgttcaccca tggtatggct gatgtcaaag
     4801 cactccatcc gcttcacttc cggcaatttc aacacgctgg caagtgctgt aagccgctgg
     4861 tgaacggtag attgctgcga aagtttgctg gttaaggccg tcgccgcatt ggtgcgcgcg
     4921 agtttcaggt aacgcgccct atcgccacga ggtttggttt gaacattaat cttgcgtccc
     4981 gccagttctg aaagggaatc ggcgagcagc gttttatcgc taagattaaa atcgagcagg
     5041 atctcacccg gtaaggtgcg catctggctg ccttgtaaat agaactggcc tacgaaggtt
     5101 tctaccacct cgctcagttc cgtaccgcca ggcactttcg ggaaatagct gcggctgccg
     5161 agcactttgc cctgacgaat gaacaatacg tggacacaag ccatacccgc atcgaacgcc
     5221 acaccaataa cgtcgaggtc gtcgccggta ttggaaacga attgtttttc ggtgacgcgt
     5281 cgcaccgcct gaatttggtc gcgaatacgt gccgcttctt caaactccag attctgactg
     5341 gcagtttcca tacggctaat gagttgcgta agcacctgat catctttgcc agacaaaaac
     5401 aggcgcacat actcgacctg ctgagcgtat tcttcttcac tcaccagtcc ttcaacgcac
     5461 ggtcccagac agcgccctat ctgatattgc agacacggac gcgagcgatt gcgataaaca
     5521 ctattttcgc actggcgaat ggggaaaatc ttttgcagta gcgccagtgt ttcacgtacg
     5581 gcatagccat tcgggaacgg gccgaaatat tcacctttgg catgctttgc accacgatgc
     5641 atcgccagac gcgggtgggt atcgccactc aggaagataa aaggatatga tttatcatcg
     5701 cgtagcaaaa cgttgtaacg cggctgatag agtttgatgt agttgtgttc cagcaacagc
     5761 gcttcggttt ctgtatgagt aaccgttaca tcaatttgct ggatctgggc gaccagcgct
     5821 tcggttttgc gcgaagcgag gttgctacgg aaatagctgg aaagccgttt tttcaggtct
     5881 ttcgctttgc cgacatagat aaccgtacca ccagcatcgt acatgcgata aacgccaggc
     5941 tggctggtta cggtttttaa aaacgctttt gcgtcaaact gatcactcac tgacttgata
     6001 atgtctccgc attacacaga ccatggcgaa ttgccaggtg agtcagctca acatcgccat
     6061 gaatgtttag tttactgaac atacgatagc ggtagctgtt caccgttttc ggactgagat
     6121 tgagctgttc tgagatctca ttgaccttct ggcccttcgt gatcatcagc ataatctgca
     6181 attcacgttc agacaaactg gcaaatgggc tttctgtttt ttctggttcg atctggctta
     6241 atgccatttg ttgagcgatg tcagaagcaa tgtaacgctg cccagaatag acagaacgga
     6301 tcgcactcac gacttcctgc ggagccgcgc ctttgctgag gtagcccgca gcaccggcct
     6361 gcatgacttt cgctggtaaa gggttttctg tatggacggt aagcatgatg atttttacat
     6421 cagctgtgga acgcgcgatt ttacgcgtcg cctcaagacc gccaatgccc ggcatactca
     6481 tgtccattag caccacgtca acggcatttg cccggcacca cttaacggcg tcttcaccgc
     6541 acgatgcctc accgacgact tttataccct ttatatcttc cagaatgcgt cgtatccctg
     6601 cgcgcaccag ttcgtggtca tcaacaagta gaacgttgat caaaggaata tctccagaaa
     6661 tagggataac gctactgata gttagtcatt cgtatattag cggtttttat tgcaactttg
     6721 aaacgttaaa aatggtgggt attcgatttt tctctcgttt ttgtcaattc gattgtccac
     6781 agttatggtc acgcccgtca ccaacggctt accggatcgt ttcacagtaa gccctgcgac
     6841 gaaatgtgac aaaaattacc tttattcagc aaaaatgaaa atcagcccgc tgaatatgta
     6901 acattaatta acccgaacaa tacgggcaaa aaacattcat gataaaaata tttattggtc
     6961 attatattaa cgcatttaat agcaccgttg acggcacgct caaaaaacaa ccactgctat
     7021 ttttagcaaa gcttatggta tactccgccg ccttaacatt tttcaccgca aattttcatt
     7081 gcaacatgac gaggaaaata aatgagtacg cctgattttt ctactgccga gaataatcaa
     7141 gaactggcaa atgaagtctc ctgcctgaaa gcgatgctga cgctgatgct gcaggcgatg
     7201 ggacaagctg acgcgggccg cgtgatgtta aagatggaac aacagcttgc gctgatcgaa
     7261 gacgaacccc aggctgcagt attttccaaa acggttaagc aaattaaaca agcctaccgt
     7321 cagtaatata aaaccggctg atagcgtgcc tttcagccgg tttttgcatc tggcacgcag
     7381 gacagaaaag agatcaaatt aagccagtag cggccgcgta acaggcaacc tgggtcttat
     7441 ttggtgcatt aatttttttc tgcatgtttt tctggtggaa attgaccgta ttctcagaga
     7501 ttgacaaaat catcgctatc tctgctgatg tcttcccttc cgccgtccac ctcagaattt
     7561 ctttttcgcg cttgctgaaa ttcatctctg gcgtcatcac tatttcatca tttaaacgca
     7621 tcagagccat cagactttcg cgcaccagta actgcatttt taattgcagt tcatcactaa
     7681 gaatgggtat ttcgcgcgcg ctgcaacggg aaaaggacaa aaagcccagc gcccggtttg
     7741 gcagcattaa atactgagtg acaccgcggc gtaaaccatg tgcgcgcgcg gcttcccata
     7801 acggctgtgc ttcgttgaat aagtcatcat tccacattag atggccctga ctaaagtttt
     7861 cagggttcag caccggatca attgcgagaa agttttttgc ctgataataa ctaacccacg
     7921 cctcagggta attggtgtag aaagccactt taggtcgagt gaatggtacc gggtggcgga
     7981 cacataacga atagtaatcg tactccagct gctgagcctg aagctcaatt tcatggtaga
     8041 cctcttctgc ggtctccatc ctctgaaaac gcaacagcat cgtgcgacgc cagctgaaaa
     8101 aatccgtatc ctgcatagta aaccgcaacg cccctga
//
LOCUS       DBFIFI010000193         8088 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.193, whole genome shotgun sequence.
ACCESSION   DBFIFI010000193 DBFIFI010000000
VERSION     DBFIFI010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8088
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.193"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..2808
                     /gene="evgS"
                     /locus_tag="N7B39_003955"
     CDS             <1..2808
                     /gene="evgS"
                     /locus_tag="N7B39_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HGU2423845.1"
                     /translation="LTNEVRYEVSQNWLDTGNLAFLNKPLELTEHEKQWIKQHPDLKV
                     LENPYSPPYSMTDENGSVRGVMGDILNIITLQTGLNFSPITVSHNIHAGTQLNPGGWD
                     ILPGAIYSEDRENNVLFAEAFITTPYVFVMQKAPDSEQTLKKGMKVAIPYYYELHSQL
                     KEMYPEVEWIKVDNASAAFHKVKEGELDALVATQLNSRYMIDHYYPNELYHFLIPGVP
                     NASLSFAFPRGEPELKDIINKALNAIPPSEVLRLTEKWIKMPNVTIDTWDLYSEQFYI
                     VTTLSVLLVGSSLLWGFYLLRSVRRRKVIQGDLENQISFRKALSDSLPNPTYVVNWQG
                     NVISHNSAFEHYFTADYYKNAMLPLENSESPFKDVFSNTHEVTAETKENRTIYTQVFE
                     IDNGIEKRCINHWHTLCNLPASEHAVYICGWQDITETRDLIHALEVERNKAINATVAK
                     SQFLATMSHEIRTPISSIMGFLELLSGSGLSKEQRVEAISLAYATGQSLLGLIGEILD
                     VDKIESGNYQLQPQWVDIPTLVQNTCHSFGAIAASKSIALSCSSTFPDHYLVKIDPQA
                     FKQVLSNLLSNALKFTTEGAVKITTSLVHIDDNHAVIKMTIMDSGSGLSQEEQQQLFK
                     RYSQTSAGRQQTGSGLGLMICKELIKNMQGDLSLESHPGIGTTFTITIPVEIIQQVAA
                     VEAKAEQPITLPEKLSILIADDHPTNRLLLKRQLNLLGYDVDEATDGVQALHKVSMQH
                     YDLLITDVNMPNVDGFELTRKLREQNSSLPIWGLTANAQANEREKGLNCGMNLCLFKP
                     LTLDVLKTHLSQLHQVAHIVPQYRHLDIEALKNNTANDLQLMQEILMTFQHETHKDLP
                     AAFHALEAGDNRTFHQCIHRIHGAANILNLQKLINISHQLEITPVSDDSKPEILQLLN
                     SVKEHIAELDQEIAVFCQQNN"
     gene            complement(2864..4005)
                     /gene="yfdE"
                     /locus_tag="N7B39_003956"
                     /pseudo
     CDS             complement(2864..4005)
                     /gene="yfdE"
                     /locus_tag="N7B39_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
     gene            complement(4079..5023)
                     /gene="yfdV"
                     /locus_tag="N7B39_003957"
     CDS             complement(4079..5023)
                     /gene="yfdV"
                     /locus_tag="N7B39_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HGU2423846.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(5093..6787)
                     /gene="oxc"
                     /locus_tag="N7B39_003958"
     CDS             complement(5093..6787)
                     /gene="oxc"
                     /locus_tag="N7B39_003958"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HGU2423847.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(6841..>8088)
                     /gene="frc"
                     /locus_tag="N7B39_003959"
     CDS             complement(6841..>8088)
                     /gene="frc"
                     /locus_tag="N7B39_003959"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HGU2423848.1"
                     /translation="STPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDVT
                     RHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAID
                     HMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPPL
                     VSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRLD
                     KLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQNW
                     ENTCKAIGKPDWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIPC
                     APVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEHT
                     AAVLQELGYSDDEIAAMKQNHAI"
BASE COUNT         2351 a         1885 c         1704 g         2148 t
ORIGIN      
        1 ttaacaaatg aagttcgcta tgaagtatca caaaattggc ttgatacagg aaacctggcc
       61 tttctgaaca agccattaga actcactgaa catgaaaaac agtggattaa gcagcatccc
      121 gatttaaagg tgctggaaaa tccttactcg cccccctatt ctatgacgga tgaaaatggc
      181 tcggttcggg gcgttatggg ggacattctt aatattatta ccttgcaaac aggtttaaat
      241 ttttctccga tcaccgtttc acacaatatc catgctggaa cacagctgaa tcccggcgga
      301 tgggatatat tacccggtgc tatttatagt gaagatagag aaaataatgt tttatttgct
      361 gaagccttca taacaacgcc ttacgttttt gtcatgcaaa aagcgcctga cagtgaacaa
      421 acattaaaaa aaggaatgaa agttgccatt ccatattatt atgagcttca ttcgcaatta
      481 aaagagatgt atccggaggt agagtggata aaagtcgata acgccagcgc tgcatttcac
      541 aaggtcaagg aaggcgaact tgatgctctg gtcgcgacac agttaaattc acgttacatg
      601 atcgaccatt actatcctaa tgaactgtat cattttctta ttcccggcgt tccgaatgca
      661 tcgctttcgt tcgcttttcc tcgcggagaa ccggaactta aggatattat taataaagca
      721 ctgaatgcaa tccccccaag cgaagttctg cgcctgaccg aaaaatggat taaaatgccc
      781 aatgtgacca ttgacacatg ggacctctat agcgagcaat tttatattgt tacgacatta
      841 tccgttttat tagttggcag tagcctttta tggggattct acctgttacg ctcagttcgt
      901 cgtcgtaaag tcattcaggg tgatttagaa aaccaaatat cattccggaa agcgctctcg
      961 gactccttac cgaatccaac ttatgttgta aactggcaag gtaatgtcat tagtcacaat
     1021 agtgcatttg aacattattt cactgctgat tactacaaaa atgcaatgtt gccattagaa
     1081 aacagtgaat caccctttaa agatgttttt tctaatacgc atgaagtcac agcagagacg
     1141 aaagaaaacc gaacaatata cacacaggta tttgaaattg ataatggcat cgagaaaaga
     1201 tgcattaatc actggcatac attatgtaat ctgccagcca gcgaacatgc tgtttatatt
     1261 tgtggttggc aagatattac cgagacgcgt gatttaattc atgcactcga agtagagaga
     1321 aataaagcga tcaatgcaac tgtcgcaaaa agtcagtttc tggcaacaat gagtcacgaa
     1381 ataagaacac caataagctc cattatgggc ttcctggaac ttctgtcggg ttctggtctt
     1441 agcaaggagc aacgggtgga ggcgatttca ctagcctacg ccaccggaca atcactcctc
     1501 ggcttaattg gtgaaatcct tgatgtcgac aaaattgaat cgggtaacta tcaacttcaa
     1561 ccacaatggg tcgatatccc tactttagtc cagaacactt gtcactcttt cggtgcgatt
     1621 gctgcaagca aatcgatcgc attaagttgc agcagtacat ttcctgatca ttacctggtt
     1681 aagatcgacc ctcaggcgtt taagcaggtc ttatcaaatt tactgagtaa tgctctcaaa
     1741 tttaccaccg agggggcagt aaaaattacg acctccctgg ttcacattga tgacaaccac
     1801 gctgtaatca aaatgacgat tatggattct ggaagtggat tatcacagga agaacaacaa
     1861 caactgttta aacgttatag ccaaacaagt gcaggtcgtc agcaaacagg ttctggttta
     1921 ggcttaatga tctgcaaaga attaattaaa aacatgcagg gtgatttgtc attagaaagt
     1981 catccaggca taggaacaac atttacgatc acaatcccgg tagaaattat ccaacaagtg
     2041 gcggctgtcg aggcaaaagc agaacaaccc atcacactac ctgaaaagtt gagcatatta
     2101 atcgcggatg atcatccgac caacaggcta ttactcaaac gccagctaaa tctattagga
     2161 tatgatgttg atgaagccac tgatggtgtg caagcgctac acaaagtcag tatgcaacat
     2221 tatgatctgc ttattactga cgttaatatg ccgaatgtgg atggttttga gttgactcgc
     2281 aaactccgtg agcaaaattc gtccttaccc atctgggggc ttacagccaa cgcacaggct
     2341 aacgaacgtg aaaaagggtt aaattgcggc atgaacttat gtttgttcaa accgttgacc
     2401 ctggatgtac tgaaaacaca tttaagtcag ttacaccagg ttgcgcatat tgtacctcag
     2461 tatcgccacc ttgatatcga ggccctgaaa aataatacgg cgaacgatct acaactgatg
     2521 caggagattc tcatgacttt ccagcatgaa acacataaag atttacccgc tgcgtttcat
     2581 gcactagaag ctggcgataa tagaactttc catcagtgta ttcatcgcat ccacggtgcg
     2641 gctaacatcc tgaatttgca aaagttgatt aatattagcc atcagttaga aataacacct
     2701 gtttcagatg acagtaagcc tgaaattctt cagttgctaa actctgtaaa agaacacatt
     2761 gcagagctag accaggaaat tgctgttttc tgtcaacaaa ataactaaat agtggctccc
     2821 acaatgttca aatgtgggag ctatttacca gcacatctta catttatgat gagaactcct
     2881 ggcgaatttg ctccccatgc tggtcgagcg ttgccgctcc cggcataaca tgcgggtccg
     2941 cgcagccact gatttttatc ggattacccg gcatcattat tcccccggct tcaatcaaca
     3001 tatttctcgc ctgagtttgt ggcaaattaa tggcctcagc cacacttaat aacggcgcga
     3061 cgggcacacc aacttcatgt attctggcta accaaacttc agctgcctgt gtttttaacg
     3121 tccgctcaat atattgttta agaatagtct ggttttgtac gcgtaaaata ttgctgctaa
     3181 atctgggatc attaaccagt tccgtaagct ccagtgcctg gcataacgca gagaaaagct
     3241 tgtcattacc acaacaaatc gtaatcggct tatcctgagt atcgaaaaca tcaaaaggtg
     3301 ccatgtaggg gtggcgattt cccaggcgtt gtggtgactt ccctgtcgca atatatgcca
     3361 tcagaccatg ctccagaaaa ctcagcgtgg catcaaacat cgctatatcg acatgcgccc
     3421 ctctttggct cttttcgcgg ccataaagtg cactcactat tccgctgaat aaataaacac
     3481 cgccgcatag atccgcaaga gaggtaccaa cacgcactgg cggagcatca gggtaccctg
     3541 tttccatcat tatcccgctc attgcctgaa tgatggtatc gtaggcagga gcatctttta
     3601 gcggaccggt atgtccgaaa cctgacgatg aagcatatat gaggcgcggg ttgatttctt
     3661 gtaacctttc ccatgaaagc cccagttttt ccattgtacc tgggcgaaaa ttctcagcta
     3721 atacatcagc ttgtttaagc atatttataa atatactttt atcgtgatca ttctttaaat
     3781 caagaaccac actctcttta ccatgattaa taaaactgta atagagtgtc catccacata
     3841 gggaccaaat gtgcgggtat catcaccatg acccggcggc tcaactttaa ttacccttgc
     3901 gcccatatta caaagaagtt gagttccgaa aggtccatta aggacatgtg tcatatcgat
     3961 aactaataag ccttcaaacg gccctttgct ttcattattt gtcatttgct tatgccttga
     4021 aaaaagccga aggtgaaaag acacttcggc atttatgtaa aatcaataac atgattaatt
     4081 atgaaaccag tcgactgaca taaatccaca agggagccgt gacgacaaaa cccagtacgc
     4141 tcacagccaa tgacgctgta ccagtgcggg tgtagacatt aaaccggctg gcaataatga
     4201 ttccggagaa tgccggcggt aatgcgcctg ccagtaccat catctgcaga tgttcgctgt
     4261 tcaaatgaca tgccatacca acaagaagca gcgccagtgg catcagaatc agcttcagga
     4321 aggtgttata agcaatttcg gcactgaact cgaatttatg tgcagccaga gtcaaccccg
     4381 cagcgaatac cgctacccct gagttagcct tcgcaatcag attaaaggtt gggtcccatg
     4441 ctgccggaat ttttacccca accaacacca ggatcgttgc cagaacaggt gcccatacaa
     4501 ctggctcctt tgccgcagaa attaatgcgc tcagattact attcttctta ccatccgctc
     4561 ctgaggaagg attcagcaaa tacagaccaa taggaatagt aattgcgtta acaataatag
     4621 aaataattgc taccactaaa cctgttgata cggaatcacc ataaatagga tcgagaactg
     4681 caaaccccag gaatccaatg gtaggtgaac ctgcaattaa tgcacataca gctgcttctg
     4741 catgggtacg tttaaaaaat ttgtagcaac caaaccagga gaaaaagaaa catccgacaa
     4801 taaccacgag tgatacaagg gtcagacgag tgtccgcaaa aatcatttcc ctgtttgccc
     4861 gggtaataga aacaaatagg gctgcaggaa gcgcgtagtt taataccagt ttattaaatg
     4921 cccgggcttg atcttctgaa aaagtttctc gtctgccgct aaaataaccc aacagcatga
     4981 ttacgataat aggcaataaa tcgccaataa aaaatgttag cataatagta tttccgataa
     5041 gggtcccctc accatcgcat aatgagttgg caagaggaga cgatgtcagt atttaattac
     5101 cagcgacttg ttttgggtta agtttggtaa tatggccact ttcagttcct gctgcagggt
     5161 cgatgaccac attaataatg gtcggtttgc gcgactgaat accggtggtt aaagcatgac
     5221 gaagttcatc tgtcgtggtg acgttatagc caacgccacg aaacgcatcc attaatttgt
     5281 cataccttgc atggtgcaac agatctgttg gtgatggtgc accagcgcca ctgagatcaa
     5341 caccgtctcc tctgtagatg ccgccattat taaaaataac gatcgttacc ggcaggttat
     5401 atcgacaaat cgtttcaatt tccatcccac tgaaaccaaa agcactatca ccttcaatgg
     5461 cgacaaccgg agaaccagag gtcacgctag caccgatggc atagcccata ccgatgccca
     5521 tgacacccca ggtaccacaa tccagacgac gacgtggttt atacatatca ataatatttc
     5581 gtgcattatc cagggtattt gcaccttcat taactaaata aatatcctgg ttctcacgca
     5641 atacatcgcg cacagcactt aatgcattaa agtaatttaa tggttgggta tctgtactta
     5701 atttttcatg cattttttgt gcattttgct gcttgtggat atttaaaata tcgcgccata
     5761 ccagtggagt cgtaaatgtg ttttgtttca gctctgccag cataccttgc atactggatg
     5821 caatatcgcc aacgactggc acagcaatgg ggcggttgct gtcaatttcc tgcggttcaa
     5881 tatccagttg aataaactgt gtatctgccg cccatccttt tttaccgtgt gccagtaacc
     5941 aattcagtcg tgcaccaaca agcatgacaa cgtcagcatt tgccagggca aacgaacgcg
     6001 cagctgccgc agaaagtgga tgcgtatctt cgaggatccc tttcgccata gacattggca
     6061 ggaatggaat ctgggcactt tcaataaatt cacgaagctg ttcgtcagct tgtgaatacg
     6121 ccgcgccttt gccaaggata attaatggcc gttcagcttt tgctaaaagc gaaattgcgc
     6181 tagtgactga cttcgggcat ggcaataatg ctggcgacgg attttcaact tttacaatcg
     6241 tggttaacgc ttcgtctttt tccatcgtcg cggccaggac atttgctggc aaatcaagat
     6301 aaactccgcc agggcgaccc gatactgaga cccggatagc gcgtgccaat gcaatgccaa
     6361 gatcctgcgg ctgattaacg cgaaatgctg ctttggcata cggttttgcc gcattcattt
     6421 ggtccagctc ttcataatca ccttgctgta ggtcgacgat cgcgcggtcg ctggagccgc
     6481 taatcatgat catcggaaaa ccatttaccg ttgcgttggc caatgcggtc aaaccattga
     6541 ggaatcctgg cgcagacact gtcaggcaga tccctggttt ttgggtaaga aaaccgcttg
     6601 ccgcagcggc atagcctgcc gactgctcat gacgaaaacc aatataacga atgccttccg
     6661 cctgggcatg gcgcgccata tccgtcacag gaatacctac aacaccataa atagtgtcaa
     6721 tattattctg ttttaatgct tcaacgatga tatgcatacc atctgtcatt tgaagttgat
     6781 ctgacatgat taataacctc gaaattttta taaattggga gccagaagcc cccgttaata
     6841 tcagatggcg tggttttgct tcattgcagc aatttcatcg tcgctataac ccagctcctg
     6901 caataccgca gcggtatgtt cacctaatag aggcgcagct ttaatatccg gcgtaaaggc
     6961 agagaatttc attggacaac caactgtcag atattttcca cgcaatggtt gttccacttc
     7021 gacaacactg ccgctttggc gtaaagaggg atcaagtgaa atttctttca tacttaaaac
     7081 cggtgcacaa ggaatatcaa actgagtcaa ataagccact gcttcatgtt tatcaatagt
     7141 gacagtgtat ttttcgattt cagcaaaaat atcgaaaata tgtggctgtc gggcatgggc
     7201 tgtactgtat gccggatcgg taatccaatc tggtttgccg atggctttac aggtgttttc
     7261 ccagttttgc tcctgaatag tgaaataaat ataggcgtta ggatcagttt cccagccttt
     7321 acatttcagg atccagccag gctgaccgcc accacccgca ttacctccgc ggggaactgc
     7381 atcaccaaat gtgccattcg gatactgcgg gtattcttcc agataaccca atttatcgag
     7441 acgctgctgg tcgcgtaatt tcacgcggca aaggttcaat acggcatcct gcattgacat
     7501 ggtgactcgt tgcccacgcc ccgttttttc gcgatgcagc aaagcagcaa gtaaaccgat
     7561 cagtaaatgc attccggtgt tgctgtcacc caacgctgca gcgcttacca gcggcggacc
     7621 gtcccaaaaa cccgtagtgg atgccgcgcc acccgctgcc tgagcaacgt tttcataggc
     7681 ttttacattc acataaggcg aacactcatc aaaccctttg atcgaaccaa aaatcagacg
     7741 tggattgatt tcttgaatat gctcccaggt gaagcccatg tgatcaatgg cccctggatg
     7801 aaagttctcg actaagatat cagcttcgcg gatcagcttt tccattacct ctttgccttc
     7861 cgctgttttg gtatttaact caatagaacg tttgttactg ttaagcatgg tgaagtaaag
     7921 cgcatcgata tcaggaatat ctcgcagctg gtgacgcgtt acgtcaccaa cgccggggcg
     7981 ttcaatttta atgacgtcag cgccaaacca ggccagcatt tgagtacaag atgggccaga
     8041 ttgcacaccg gtgaaatcga gaactttaat tccttgaagt ggagttga
//
LOCUS       DBFIFI010000194         8048 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.194, whole genome shotgun sequence.
ACCESSION   DBFIFI010000194 DBFIFI010000000
VERSION     DBFIFI010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8048
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.194"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            208..705
                     /gene="ubiC"
                     /locus_tag="N7B39_003960"
     CDS             208..705
                     /gene="ubiC"
                     /locus_tag="N7B39_003960"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HGU2423849.1"
                     /translation="MSHPALTQLRALRYCKEIPALEPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            718..1590
                     /gene="ubiA"
                     /locus_tag="N7B39_003961"
     CDS             718..1590
                     /gene="ubiA"
                     /locus_tag="N7B39_003961"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HGU2423850.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(1745..4168)
                     /gene="plsB"
                     /locus_tag="N7B39_003962"
     CDS             complement(1745..4168)
                     /gene="plsB"
                     /locus_tag="N7B39_003962"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HGU2423851.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPVHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4339..4707
                     /gene="dgkA"
                     /locus_tag="N7B39_003963"
     CDS             4339..4707
                     /gene="dgkA"
                     /locus_tag="N7B39_003963"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HGU2423852.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            4817..5425
                     /gene="lexA"
                     /locus_tag="N7B39_003964"
     CDS             4817..5425
                     /gene="lexA"
                     /locus_tag="N7B39_003964"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HGU2423853.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5498..6823
                     /gene="dinF"
                     /locus_tag="N7B39_003965"
     CDS             5498..6823
                     /gene="dinF"
                     /locus_tag="N7B39_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HGU2423854.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVRGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTSWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLASLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            6939..7148
                     /gene="yjbJ"
                     /locus_tag="N7B39_003966"
     CDS             6939..7148
                     /gene="yjbJ"
                     /locus_tag="N7B39_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HGU2423855.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7190..7705)
                     /gene="zur"
                     /locus_tag="N7B39_003967"
     CDS             complement(7190..7705)
                     /gene="zur"
                     /locus_tag="N7B39_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HGU2423856.1"
                     /translation="MEKTTTQELLAQADKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
BASE COUNT         1815 a         1970 c         2219 g         2044 t
ORIGIN      
        1 accgctcttg gcatcatggg cggtgatgcc cctatggttg ccctcgccag cacgggcatc
       61 ggcaaagcgt aaggttcaac agcgttttac cacttcatgc gattgttgcg tttttgttgc
      121 gcattagatc acttaatttg ctttacatct cccgtaaaca cttttctgcg atacaatgcc
      181 tttacgttat gtaacggaga gttcggcatg tcacaccccg cgttaacgca actgcgtgcg
      241 ctgcgctatt gtaaagagat ccctgccctg gagccgcaac tgctcgactg gctgttgctg
      301 gaagattcca tgacaaaacg ttttgaacag cagggaaaaa cggtgagcgt gacgatgatc
      361 cgcgaagggt ttgtcgagca gaatgaaatc cccgaagaac tgccgctgct gccgaaagag
      421 tctcgttact ggttacgtga aattttgtta tgtgccgatg gtgaaccgtg gcttgccggt
      481 cgtaccgtcg ttcctgtgtc aacgttaagc gggccggagc tggcgttaca aaaattgggt
      541 aaaacgccgt taggacgcta tctgttcaca tcatcgacat taacccggga ctttattgag
      601 ataggccgtg atgccggact gtgggggcga cgttcccgct tgcgattaag cggtaaaccg
      661 ctgttgctaa cagaactgtt tttaccggcg tcaccgttgt actaagagga aaaaaatatg
      721 gagtggagtc tgacgcagaa taagctgctg gcgtttcatc gcttaatgcg tacggataag
      781 ccaattggcg cgttactgct gctctggcca acattatggg cgttgtgggt ggcgacaccg
      841 ggcgttcccc agctctggat cctggcggtg tttgtcgcgg gtgtctggct gatgcgcgct
      901 gccggatgtg tggtgaatga ttatgctgac cgcaagtttg atggtcatgt taagcgcacg
      961 gcgaaccgac cacttcccag cggcgcggta acagagaaag aggcgcgcgc gctgtttgta
     1021 gtgctggtac tgatttcgtt tttactggtg ctgacgctga atacgatgac cattctgttg
     1081 tcgattgccg cgctagcgct ggcgtgggtg tacccgttta tgaagcggta tacccatcta
     1141 ccgcaagtgg tgctgggcgc ggcgtttggc tggtcgattc caatggcttt tgctgctgtg
     1201 agtgagtcgg tgccattgag ttgctggtta atgttcctcg ccaatattct ctgggcggtg
     1261 gcttacgaca cgcagtatgc gatggttgac cgtgatgatg atgtgaagat tggcattaaa
     1321 tccaccgcaa tcctgttcgg ccaatacgat aaattgatta ttggtatttt gcagattggc
     1381 gtactggcgc tgatggcgat tatcggtgag ttaaatggct taggctgggg atattactgg
     1441 tcaattctgg tggctggcgc gctgtttgtt tatcaacaaa aactgattgc caaccgcgag
     1501 cgcgaagcct gctttaaagc atttatgaat aataactatg ttggtctggt actgttttta
     1561 gggctggcaa tgagttactg gcatttctga tgatgtaaaa aagccggatg atcatccggc
     1621 tttcttctgg gttgcctgat gcgctgcgct tctcaggcct acacaacaga tcgcaattta
     1681 ttgaatttgc agattatgga aggccggata aggcgtttgc gccgcatccg gcaattctct
     1741 ctgattaccc ttcgccctgc gtcgcactct caatcgtcaa acgcacgtct gatgtaatca
     1801 actccgccag caactgataa accttcatcg tttctgccgg ttcggcatcg ccgctatcgc
     1861 tgatataccc ttcatcacgc agcgtaagca ccagagaact gaacaccgcc ttgtcgaaga
     1921 actccggcgc gttgatgccg tgcagcacgg agagacgttg cgcgacggtg cggctctctt
     1981 tctccagcgt accgcggttg atcgacgggt tggcgctcaa cagccagaag gtgatggcat
     2041 aacgttgcag cgtttcgcgc gcgcctgcgg cgagcagctg tagcgtgcga gaatgcaccg
     2101 ggttgatatg caactcatca tcttgcaggg taatcagccc ctgacgttgc atctcatttg
     2161 ccagcgcatc aataacgtcc ggcaactcgt cgcgatccca gcgcaggaac agctccgctt
     2221 tcagcattgg gtaaagcaca ttgacgtgct ccatcaatac gtcgcgggag atgtggcgat
     2281 gctgggtgac aattgccgcc atcagcgaag gtagcaccaa catatgcgca atgttgttgc
     2341 gatagtaggt catcagcacc gcttgctcgc gcggcagaat gatgatgtcg ccgatagtgt
     2401 ctttctcaac ttcaaacttg ttcatttgca gcgcgtgatc gataagctcg ctggcgctgg
     2461 ctgaaggaac ggtagagtcc gtggagtaag gcacgttgcg catcagatcc aggtagcagt
     2521 tgagttgctc ggttaactgc tcgcgggtga gtgagcgctg acgtgatgcc agtagcgcag
     2581 tacagcacag gttcatggcg tttgccgcgc ctgcgttgtt aatgcgtacc atcagatcgg
     2641 cagcaatatt attgaccgtc ggcgttaacc atgccggacg cactgcttcg atgggatcga
     2701 tagattcacg ccagtctggt acgtgctggt taaggtaggt catcaacggc attggttcac
     2761 cgaaattgac gtaaccctgg ccgagattac gcagcttgct taaaccgcgc agcatctgcg
     2821 gcaggctctc tttctctttc gtcgcgccgc gcagttcttt ggcgtaagta cccacttcca
     2881 tgacgtgctc ataaccgata tagatcggaa tcagcgtaat cggacgcgtg ccgccacgca
     2941 gcatcgcctg aatggtcatc gacagcgtac cggttttcgg atccagcaaa cgccccgtac
     3001 gggaacgacc gccttctacg aagtactcga cggaataacc acggctgaac agttcgccga
     3061 gatactcacg gaaaacggtg gaatagagct tattgccttt aaaagtacgg cgaataaaga
     3121 acgcacccag acggcggaaa atcggcccgg caggccagaa attcaggttg atcccggcgg
     3181 cgatatgcgg cggcactagc ccctggtggt acagcacgta agaaagcagc aggtagtcca
     3241 tgtgactgcg gtggcaaggc acatatacca gctcatgacc gtcgtgggcc agctggcgaa
     3301 cgcgctcggc gttatggacg ttgatgccct ggtaaaggcg gttccaggta aagcccagaa
     3361 tacggtcagt caggcggatc atctcgtaag agaaattcgc cgcaatctct tccatcagcg
     3421 caatcgcgtt ctgctgcgct ttttcatggg agattttttt gctgcgcgct tcatcttcta
     3481 ccgctttggc aatggcgcgg gaggcgagca gcttattaaa cagatcctga cgagcaggca
     3541 gacgtggtcc tacggcagcc agacgttgac gggcaaagtg catacgcgcc acgcgcgcca
     3601 gtttctgcgc gatagtttta tccgtgccgt gttcatccgc catacggcgc agcgaaactg
     3661 acggcgagaa acgcacaaaa ctgtcgcgac cgagccacag tacagcgaaa aatttctgta
     3721 cgccgttaag catacgcagc ggcgggttca cttcgccttt ttcacgcccc ggcgcgcgac
     3781 caaacatcac cgacactggc accatctgca catccagatt tgggttgcta cggtgcaaat
     3841 cgagatagtc gtggaacagc ttaatagact cttctttcgg cgtgtaatag gtgaatacac
     3901 gcggcccgcc gtgaatgaac acatagcgcg gcagtagcgt gccgtcgatt tccagcggct
     3961 ctaacgggtc aggcaagtca tgtgccagac actgggcgcg caacgtcagc aaatctgctt
     4021 tcgagttgta cggtaaaacg tacataattg gacgagaggt atccagcccc agttccgggg
     4081 caggatctgc cggaatagac ttgcttttta ccaggatgct taatggtaaa ttcagtaatt
     4141 tgtagtaaat tcgtggccag ccggacataa acgatgtaaa gcctctggtt aataatgcaa
     4201 atgcgcggca aggatagcag aaagtcatgg gaaattctgt ggtatccgct catgtttcgc
     4261 gcggcgttac gcaaacccga atcatcggat ttaacggtac actgatattg acgctcataa
     4321 tgtaaaaagg ttctttcaat ggccaataat accactggat tcacccgaat aatcaaagct
     4381 gctggctatt cctggaaagg tttacgcgct gcatggatca acgaagcggc attccgtcag
     4441 gaaggcgtag cggtattgtt ggcggtggtc atcgcctgct ggctggatgt ggacgcgatt
     4501 acccgcgtgc tgcttatcag ctccgtgatg ctggtgatga ttgtggaaat cctcaatagc
     4561 gccatcgaag cagtggttga ccgaattggc tctgaatacc atgagctttc cggacgcgct
     4621 aaagatatgg gatccgccgc ggtgctgatt gcgattatcg tcgccgtgat tacctggtgc
     4681 atcctgttat ggtcgcattt tggataaccc ttccagaatt cgataaatct ctggtttatt
     4741 gtgcagttta tggttccaaa atcgcctttt gctgtatata ctcacagcat aactgtatat
     4801 acacccaggg ggcggaatga aagcgttaac ggccaggcaa caagaggtgt ttgatctcat
     4861 ccgtgatcac atcagccaga caggtatgcc gccgacgcgt gcggaaatcg cgcagcgttt
     4921 ggggttccgt tccccaaacg cggctgaaga acatctgaag gcgctggcac gcaaaggcgt
     4981 aattgaaatt gtttccggcg catcacgcgg gattcgtctg ttgcaggaag aggaagaagg
     5041 gttgccactg gtaggtcgtg tggctgccgg tgaaccgctt ctggcgcaac agcatattga
     5101 aggtcattat caggtcgatc cttccttgtt caagccgaat gctgatttcc tgctgcgcgt
     5161 cagcgggatg tcgatgaaag atatcggcat tatggatggc gacttgctgg cagtgcataa
     5221 aactcaggat gtacgtaacg gtcaggtcgt tgtcgcacgt attgatgacg aagttaccgt
     5281 taagcgcctg aaaaaacagg gcaataaagt cgaactgttg ccagaaaata gcgagtttaa
     5341 accaattgtc gttgaccttc gtcagcagag cttcaccatt gaagggctgg cggttggcgt
     5401 tattcgcaac ggcgactggc tgtaacatat ctctgagacc gcgatgccgc ttggcgtcgc
     5461 ggtttgtttt tcatctctct tcatcaggct tgtctgcatg gcattcctca cttcatctga
     5521 taaagcactt tggcatctcg ccttacccat gattttctcc aatatcactg ttccgttgct
     5581 gggactggtc gatacggcgg ttattggtca tcttgatagt ccggtttatt tgggcggcgt
     5641 ggcggttggt gcaacggcga ccagctttct ctttatgctg ttgctgtttt tacgcatgag
     5701 caccaccggg ctgactgcgc aggcttatgg tgccaaaaat cctcaggcat tagcccgtgc
     5761 gctggtgcaa cctttgctgt tggcgttggg ggctggggcg ttaattgcgc tgctgcgtac
     5821 gccgattatc gatctggcgc tgcatattgt tggcggtagt gaagcagtcc tggaacaggc
     5881 gcggcgcttt cttgaaatcc gctggttaag cgcaccggcg tcgctggcga atctggtatt
     5941 acttggttgg ttactcggcg tgcaatatgc ccgtgcgcca gtaattttgt tagtggtcgg
     6001 caatatcctc aacattgtgc tggatgtctg gctggtgatg ggactgcata tgaacgtgcg
     6061 gggcgcggcg ctggcgacgg ttattgcgga atatgcaaca ttgctgattg gtctgctaat
     6121 ggtgcgtaaa atcctcaaac tacgcggaat ctccggcgaa atgctgaaaa cttcctggcg
     6181 aggaaacttc cgtcgcttgc tggcacttaa ccgcgatatc atgctgcgct cgctgttgtt
     6241 gcaactctgt ttcggcgcga tcaccgtact tggcgcgcga ctggggagtg acattatcgc
     6301 tgttaacgcg gttctgatga cgctactcac ctttaccgcc tatgcgctgg acggttttgc
     6361 ctacgcggtt gaagcacatt ccggtcaggc atacggtgcg cgcgacggta gccagttgct
     6421 ggatgtctgg cgggcagcat gccgccagtc ggggatcgta gcgttactgt tttcggtggt
     6481 ttatttgctg gcaggggaac acatcattgc gttactggcg tcgttaaccc agattcagca
     6541 gctggccgac cgctatctta tctggcaggt gattttgccg ttggtcggcg tctggtgtta
     6601 tctgctggac ggcatgttta taggcgcaac gcgtgctgct gaaatgcgta acagtatggc
     6661 ggtggccgcc gcaggttttg cgctgacgct ccttacgctg ccgtggctgg gtaatcatgg
     6721 tttgtggttg gcgttaaccg tctttctggc gttgcgcggg ctttctctgg cagctatctg
     6781 gcggcgtcac tggcgcaacg gtacctggtt tgccgcaacg tgacggttaa aaattctgaa
     6841 taaataatcc taagccaaat tgctgactac acttaatctc acgttcagaa gaaaagtgaa
     6901 cgtactctca ttcacaacct aacgatgagg tcttgattat gaataaagat gaagccggcg
     6961 gtaactggaa acagtttaaa ggtaaagtga aagagcaatg gggcaaactg accgatgatg
     7021 atatgacgat cattgaaggt aaacgtgatc aactggtcgg taaaatccag gaacgttatg
     7081 gttatcagaa agatcaggca gaaaaagagg tcgtggattg ggaaacccgc aatgaatatc
     7141 gctggtaatt aatccctcct gcccgacgtg tacaaggatg tacgccctct taacgcggtt
     7201 tctttttcac ctgcacagag tgatcatgct ggcactgttc aggatgacga cacgcttcca
     7261 cttccacgca tgccgcacag agcccatgtg cttcaatcac attatgccgc agggcaaacc
     7321 ccatttttgc cgccagcgta tgcatgatgt cttccacgcc ttcagcacat tcttctttca
     7381 ccgcgccgca gcgatcgcaa ataaacatag ctgacgtatg ggtgggctgg tcgaacagat
     7441 ggcagagcac ataactgttg gtggattcca ccttatgcac aaaaccttgc tcaagcagaa
     7501 aatccagcgc gcgataaacc gttggcggct tggcttgcgg ttcagcttcg cgcagcaaat
     7561 caagcagatc ataagcgctg atagcgccat cctgcagact catcaggcgc aacacttcca
     7621 ggcgctgtgg ggtcaggcgc acattacgct gcgcgcagat tttgtcagcc tgcgctaata
     7681 actcctgcgt tgtggtcttt tccatcgggc acctcaaagt gggggttaag aatgtcctta
     7741 ctttaccatg ttccaggaaa aacaccgaga acccttattg ttgccgtaat gttgcttttc
     7801 tgttttgtag gtaagatgtt gtgttgcctg gcagtaccat tatcaacacg ataataattc
     7861 atttttaaat aaattgcttc aagttaactc gctatttatt taaccgtggc taatcgtata
     7921 cccgaatttc agaaaattat gagcgatttt tgatagttac cggttccaaa aacacttata
     7981 aggaggacac ggtacgacaa aaaaggtaga tgaatcatgt taaagataat acccggtgca
     8041 acaggata
//
LOCUS       DBFIFI010000195         8026 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.195, whole genome shotgun sequence.
ACCESSION   DBFIFI010000195 DBFIFI010000000
VERSION     DBFIFI010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8026
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.195"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..620
                     /locus_tag="N7B39_003968"
     CDS             <1..620
                     /locus_tag="N7B39_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HGU2423857.1"
                     /translation="KHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAYGKFMST
                     IFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIYECDHFV
                     YPQYKLGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRITKVNDET
                     VSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(586..708)
                     /locus_tag="N7B39_003969"
     CDS             complement(586..708)
                     /locus_tag="N7B39_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2423858.1"
                     /translation="MTKHSTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            911..2596
                     /gene="yddA"
                     /locus_tag="N7B39_003970"
     CDS             911..2596
                     /gene="yddA"
                     /locus_tag="N7B39_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HGU2423859.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGMLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELSDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVADASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKTGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISAVL"
     gene            2634..5006
                     /gene="yddB"
                     /locus_tag="N7B39_003971"
     CDS             2634..5006
                     /gene="yddB"
                     /locus_tag="N7B39_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HGU2423860.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKFKRFNADDSSVKLGYRTTRSDWLTSHINENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGMAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGNVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADRISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHISQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            5063..7846
                     /gene="pqqL"
                     /locus_tag="N7B39_003972"
     CDS             5063..7846
                     /gene="pqqL"
                     /locus_tag="N7B39_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HGU2423861.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLLQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMEELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNEVAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLTKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
BASE COUNT         2342 a         1769 c         1789 g         2126 t
ORIGIN      
        1 gtaaacatat gcaatttatc gaattgctcg aaaccgggac gccgaatatt gatttcagtg
       61 gtcatagtga gaacacattc cgtatcattg atttttctgt gcctcccacg gcttatggca
      121 agtttatgtc aaccattttt atgcaatggg ttaaaaacga tgtgggtgaa attttcatcc
      181 gtcagtttga aagctttgtc agccgttttt tggggaatgg gcataccagt tgtattttcc
      241 aggagtcctg caaggataat ctggttgttg aaagtaatgg agatatttac gaatgcgatc
      301 attttgtcta tccacagtac aaacttggaa acattaataa atctgaactt aaaacgatga
      361 acagtgtaca actgacagcg caaaaaaaac ggatttcagc gaaatgtcag caatgtgcat
      421 ataaacctat ctgcaatggc ggttgcccta agcatcgtat tactaaagta aacgatgaga
      481 ctgtttccta tttttgcgaa ggttataaaa tccttttttc aaccatggta ccttatatga
      541 acgccatggt ggagttagcc aagaacagag taccgcttta ccacattatg gatgttgcaa
      601 aacaaatgga gaataattaa taacgctctc ttttagctcc attcttagcc tccttcttat
      661 gaccttaaca aggcacccga cataaattcc tgtcgagtgt ttcgtcatca tcatcttccc
      721 ttgcgaaaat cttggcttac gtggtatgcg tcagggcatt agcaataccc ttgattatca
      781 atagcataac cataaccact gaaaattaaa aatttctata tatttcatat tgttaaatga
      841 tacttaaata aatttcatca gtaaatcatt gccatagata atcattggat ggcacaatca
      901 attttcgcta atgataacca ttcccattac gttttgtatg ttgatagcaa agtatctctg
      961 tctgctgaag cccttttggc tgcgtaagaa taataaaact tcagtgttgt taatcattat
     1021 tatcctcgcc atgatccttg gcgtggttaa aattcaggtc tggttaaacg actggaataa
     1081 tgattttttc aatgcgctaa gccagaaaga aaccgataag ctttggcagc ttgtcctttg
     1141 gttccctgcc ctgctgggga tctttgtatt aatatccgta aacaaaacat ggttaattaa
     1201 actactgact attcgctggc gcgaatggtt aacagattac taccttaatc gctggtttgc
     1261 agataaaaac tactacttca cgcagatcta cggcgagcat aaaaatacag ataaccctga
     1321 ccaacgtatc gctgaagaca ttctcttact gatcagcaaa acgctcagtc tttcatttgg
     1381 cttcatccag tcacttagca tgctgatcac ctttaccgtt attctttggc aaagcgcggg
     1441 tatgctctca ttcactgttg gtggaacaga atggaatatc caggggtata tggtctatac
     1501 cgttgtgctc attgtgatcg gtggaacttt atttacccat aaggtgggta aacgtattcg
     1561 tccgcttaat gtggaaaaac aacgtagcga agcgacattt cgaactaatc ttgtgcagca
     1621 taataagcag gcagaactga ttgcgctaag caatgctgag tctctacaac gccaggagtt
     1681 gagcgacaat tttcatacca ttaaagagaa ctggcatcgt ttaatgaatc gtcagcggtg
     1741 gcttgattac tggcagaata tttattcgcg ctcgcttagc gttcttccct actttctgtt
     1801 attgccgcag tttattagcg ggcagatcaa tctgggcgga ctgatgaaat cgcgccaggc
     1861 atttatgctg gtatcgaaca atctaagctg gtttatttat aaatatgatg aacttgctga
     1921 actggctgcg gttatcgatc gcttgtatga gttccatcaa ctcaccgaac agcgccctac
     1981 gaataagcct aaaaattgcc aacatgcggt acaagtggct gacgcgagta ttcgtactcc
     2041 tgataataag atcatattag agaacctgaa ctttcatgtt tcgccaggca aatggctatt
     2101 actgaaaggt tactctggtg cgggaaaaac cacactgctt aaaacattat cccactgctg
     2161 gccgtggttt aaaggtgata tttcttctcc tgccgacagt tggtatgtgt cacaaacacc
     2221 gttaatcaaa accggcttac tgaaagagat tatttgtaaa gcacttcccc tgcccgtaga
     2281 cgataaatca ttgagcgaag tactgcatca ggttggtctt gggaaattgg ctgcgcgtat
     2341 tcatgaccac gatcgctggg gagatattct ttccagcggc gaaaaacaac gtatcgccct
     2401 ggcacgatta attttacgac gtccgaaatg gatatttctt gacgaaacta cctctcatct
     2461 tgaggaacaa gaggctatcc gcttactgcg tctagtgcgt gaaaaactac ccacaagcgg
     2521 cgtcattatg gttacacatc aacccggtgt ctggaacctg gccgatgata tttgtgacat
     2581 tagcgcggtt ttataaaaac aataaaagcc ctgctgtacg gagatataaa taaatgaagc
     2641 gagttcttat tcctggcgtc attttatgtg gcgctgatgt ggcgcaggcc gtcgatgaca
     2701 aaaacatgta catgcatttt tttgaagaga tgacggtcta tgctcctgtc cctgtacccg
     2761 taaacggcaa cacgcattac accagtgaaa gcatcgagcg tttaccgacc gggaatggca
     2821 atatcagcga tctgctgaga accaaccctg cggtacgcat ggattcaacg caaagtacct
     2881 cgttgaacca gggagatatt cgcccggaga aaatctctat tcacggtgcg tcgccctatc
     2941 agaatgccta tttgattgac ggtattagtg ccactaataa cctgaaccca gcgaatgagt
     3001 ccgatgccag tagtgcaacc aatattagcg ggatgtcaca ggggtattat cttgatgtca
     3061 gcttactgga caatgtgacg ctttatgaca gttttgtgcc ggttgaattc ggtcgcttca
     3121 atggcggggt aattgatgca aagttcaaac gcttcaacgc tgatgatagc agcgtgaaac
     3181 tgggttatcg cactacgcgt tcggactggt taacatcgca tatcaatgag aataacaaga
     3241 gcgcatttaa tcaaggctct tcaggaagta cttattactc cccagatttt aaaaagaact
     3301 tttatacctt gtcgtttaat caggaactcg ctgataactt tggcgttacc gccggtttat
     3361 cgcgccgcca gtctgatatc acccgcgcgg attatgtttc gaatgacggc attgtcgccg
     3421 gtcgggcaca gtataaaaac gttatcgata ctgcattgag caaatttacc tggtttgcca
     3481 gcgaccgctt tacccacgat ttaaccttaa aatataccgg ctccagccgt gattataata
     3541 ccagcacctt cccgcagtct gatcgcgaaa tgggtaataa atcctatggt atggcatggg
     3601 atatggatac gcagctcgca tgggccaaac tacgtaccac cgttggttgg gatcatatta
     3661 gtgattatac ccgtcacgat catgacatct ggtacaccga actttcatgt acatatggtg
     3721 atattacagg gcgttgcacc cgtggcggat taggacacat ttcccaggct gtagataatt
     3781 acaccttcaa aacacgcctg gactggcaaa aattcgccgt gggtaatgtt tcgcatcaac
     3841 cctacttcgg cgcggaatac atctattccg atgcgtggac tgaacgccat aaccagtctg
     3901 aatcctatgt gattaatgct gccggaaaga aaactaacca taccatttac cataaaggta
     3961 aaggcagcct gggaattgac aactacacac tgtatatggc ggatcgcatt agctggcgta
     4021 atgtgtcatt aatgcccggc gtgcggtatg actatgacaa ctatctgtca aaccacaata
     4081 tctccccgcg ctttatgacg gaatgggata tttttgctga tcaaacctca atgattacag
     4141 caggttataa ccgttactat ggcgggaata ttcttgatat gggattacgt gatatccgca
     4201 atagctggac ggaatcggta tcaggtaata aaaccctgac gcgttatcag gatttgaaaa
     4261 cgccttataa cgatgaactg gcaatgggat tgcagcaaaa aatcggtaag aacgttattg
     4321 cgcgcgcaaa ctatgtttac cgtgaagcgc atgatcaaat cagcaaaagc agtcgtaccg
     4381 acagcgcgac taaaaccacc attactgaat ataacaacga cggcaaaacc aaaacgcatt
     4441 cgttcaacct cagttttgaa ctggccgaac ccctgcatat cagccaggta gatattaacc
     4501 cgcaaattgt ctttagctat atcaagagca agggcaactt gtcgttaaac aatggttatg
     4561 aggagagcaa taccggtgat aaccaggtgg tttataacgg taatcttgtc tcttacgata
     4621 gcgttccagt ggcagatttt aataacccat taaagatctc cttaaacatg gatttcacgc
     4681 atcaaccgag cgggttggtg tgggcgaata cgctggcctg gcaagaagcg cgtaaagctc
     4741 gcattatcct gggtaagaca aatgcgcaat acatcagcga atattcagat tacaagcagt
     4801 atgttgacga aaaactggat agcagcctga cctgggacac ccgcttgtcc tggacgccac
     4861 aatttctgaa acaacaaaac ctgacgatca gtgccgatat tctcaatgta ctggatagca
     4921 aaaccgcggt tgatacaacg aataccggtg tggcgaccta cgccagtggc cgtactttct
     4981 ggcttgatgt cagcatgaaa ttttaatagt tcggatttta cttaaccggg taaattcgcc
     5041 cggttttcgc atggagataa ttatgagaaa cctctgtttc ttactgacgt tagtggcaac
     5101 tctgttgctc cccgggcggc tgattgccgc cgccttaccg caggatgaaa agttaattac
     5161 cgggcaactg gacaatggct tgcgatatat gatttatccg catgctcatc caaaggatca
     5221 ggtaaattta tggctgcaaa ttcataccgg ttcattgcag gaagaagaca atgagcgcgg
     5281 cgtggctcat tttgtagaac atatgatgtt taacggcaca aaaacatggc cgggtaataa
     5341 agtcatcgaa acatttgagt caatgggcct gcgttttggt cgcgatgtta atgcctatac
     5401 cagctatgac gaaacggtgt atcaggtgag tttgccaact acgcagaaac aaaatctgca
     5461 acaagtgatg gcaatcttca gtgaatggag taatgccgca acctttgaaa aactcgaagt
     5521 agacgctgaa cgcggcgtaa ttactgagga atggcgtgcc catcaggatg cgaaatggcg
     5581 cacctctcag gcgcgccgcc ctttcctgct ggcaaatacc cgtaatttag accgtgaacc
     5641 tatcggcctg atggatactg tcgccacggt cacaccggca caattgctcc aattttatca
     5701 acgctggtat caaccaaata atatgacctt tatcgtggtt ggcgatatcg acagtaaaga
     5761 agcgctggcg ctgataaagg ataatttaag taagcttccg gctaacaaag cagctgaaaa
     5821 tcgcgtctgg ccgacaaaag ccgaaaacca cctgcgcttt aatatcatta atgataaaga
     5881 aaaccgggtg aacggcatcg cactctatta tcgcctgcca atggtacaag tgaacgatga
     5941 gcaaagcttt atcgaacaag ctgaatggag catgttagtt cagctgttca atcaacgtct
     6001 gcaggaacgt atacagtcgg gcgagttgaa gactatttct ggcggcactg cgcgcagcgt
     6061 taaaattgca cccgattatc agtcgctgtt tttccgtgta aatgcacgag acgataatat
     6121 gcaggatgct gcgaatgcat taatggaaga gttggcaacc attgatcagc atggtttttc
     6181 tgctgaagaa ctcgatgatg tcaaatctac ccgcctcacc tggctgaaaa atgcggttga
     6241 tcagcaagct gaacgtgatt tacgtatgct gaccagtcgc ctggcatcca gctcattaaa
     6301 taatacgccg ttcttgtcgc cggaagagac atatcaactt tcgaaacgtc tgtggcagca
     6361 aattaccgtg caaagtctgg cggaaaaatg gcagcagtta agaaagaacc aggacgcatt
     6421 ttgggagcaa atggtaaaca atgaggttgc cgccaaaaaa gcattgtctc ctgcggctat
     6481 cctggcgctg gaaaaggagt acgccaacaa aaagctggcg gcttacatct tcccaggcag
     6541 aaatttatcg ttaacagtag acgctgaccc acaggcggaa attagcagca aagaaacgct
     6601 ggctgagaat ctgacatcat taacactttc caatggtgca agggttattc tggcaaaatc
     6661 cgcgggtgaa gagcaaaagc tacaaattat tgccgtatcg aataaaggcg atttaagttt
     6721 ccctgcgcag caaaaatcac ttatcgcgct ggcaaataaa gcagttagcg gaagcggcgt
     6781 tggcgaactc tcctcttcca gcctgaaacg ctggagtgcg gaaaactcgg taaccatgag
     6841 cagtaaagtc agtggcatga atacgttgct ctccgttagc gcgcggacta ataaccctga
     6901 acctggtttt cagttgatta accagcgaat cacccacagc acgattaacg acaatatttg
     6961 ggcatcgcta caaaatgctc aaattcaggc attgaaaacg ctcgaccagc gtccagcgga
     7021 gaaattcgcc cagcagatgt atgagacgcg ctatgctgat gaccgcacga aattactgca
     7081 agaaaatcag attgcacagt ttactgccgc agatgcgctg gctgccgatc gccagttgtt
     7141 ttcatctcca gcggatatca cgtttgtcat tgtcggtaat gtcgcagaag acaaactcgt
     7201 ggcgttaatt acgcgttact taggatcaat caaacactct gattcgccat tagccgcagg
     7261 taaaccatta actcgcgcga cggacaacgc atcggttact gtaaaggaac aaaatgaacc
     7321 tgtggcacag gtttcacagt ggaagcgtta tgattcccgg acacctgtta atctggcgac
     7381 gcgtatggcg ctcgatgctt ttaacgtcgc actggcaaaa gatctacgcg ttaatattcg
     7441 tgaacaggca tctggggcat acagcgtttc ttctcgcctc tcggttgatc ctcaggctaa
     7501 agatatcagt catttgctgg cttttacttg tcaaccagaa cgacatgatg aactgttaac
     7561 gttagcgaat gaagtgatgg ttaagcgttt gactaaaggg atcagtgagc aagaactgaa
     7621 cgaataccag caaaacgttc agcgcagcct cgatatccaa cagcgtagcg ttcaacaatt
     7681 agcgaacact attgtaaata gtcttattca atatgacgat cctgcagcat ggactgagca
     7741 ggagcaattg ttgaaacaaa tgacggtaga gaatgttaac actgccgtta aacaatatct
     7801 ttctcatccg gtaaatactt ataccggagt attattgcca aaataataac agccccgtca
     7861 acacatcgtt ggcggggatt ttagcaatat tcgctatttt tatgtaataa ttttataaat
     7921 gcgttcaaaa taataaccgc gtactaatag tgatatttta aggtctgatt tttacgtgat
     7981 aattcaggag gcacagaata cgcataaaaa taacagcata aaacac
//
LOCUS       DBFIFI010000196         7985 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.196, whole genome shotgun sequence.
ACCESSION   DBFIFI010000196 DBFIFI010000000
VERSION     DBFIFI010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7985
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.196"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            157..563
                     /gene="ipbA"
                     /locus_tag="N7B39_003973"
                     /gene_synonym="fimX"
                     /pseudo
     CDS             157..563
                     /gene="ipbA"
                     /locus_tag="N7B39_003973"
                     /gene_synonym="fimX"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="IpbA is also known as HbiF and FimX; frameshifted;
                     incomplete; partial in the middle of a contig; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase IpbA"
     gene            989..1783
                     /gene="hyxR"
                     /locus_tag="N7B39_003974"
                     /pseudo
     CDS             989..1783
                     /gene="hyxR"
                     /locus_tag="N7B39_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304859.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator HyxR"
     gene            1937..2698
                     /gene="hyxA"
                     /locus_tag="N7B39_003975"
     CDS             1937..2698
                     /gene="hyxA"
                     /locus_tag="N7B39_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein HyxA"
                     /protein_id="HGU2423862.1"
                     /translation="MRFVLFCPSGIVPAQFAALSTGSVGNVTCIGTEEELRNKLRHRP
                     QSVVISAGRPAECAEMWFRFYRDHSFVVVLCVAPFFLPPDVSISGVLKNLRLLKPGMS
                     VEHVISIANSSGGLSGLKHAENLPVMDSYSVFMKEVNNRTKTIVMSERFPEKQKKVLS
                     LLLAGHSWEYSAQFLKTGIRQIWLAEQSLKKRWGIPDSMSLREALLLSSNKFHGDGNA
                     LEKTNAMTLRENGNTNRYSVVVNAGTQVLSLHKYK"
     gene            2740..3822
                     /locus_tag="N7B39_003976"
                     /pseudo
     CDS             2740..3822
                     /locus_tag="N7B39_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798070.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
     gene            3812..4000
                     /locus_tag="N7B39_003977"
     CDS             3812..4000
                     /locus_tag="N7B39_003977"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HGU2423863.1"
                     /translation="MLWLRLGDESVNASIDGAAFRVGAGVQADITKNMGAYASLDYTK
                     GDDIENPLQGVVGINVTW"
     gene            4182..4850
                     /gene="ykgH"
                     /locus_tag="N7B39_003978"
                     /pseudo
     CDS             4182..4850
                     /gene="ykgH"
                     /locus_tag="N7B39_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgH"
     gene            complement(5093..5788)
                     /gene="ykgG"
                     /locus_tag="N7B39_003979"
     CDS             complement(5093..5788)
                     /gene="ykgG"
                     /locus_tag="N7B39_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HGU2423864.1"
                     /translation="MDNRGEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAKNISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAERLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(5781..7208)
                     /gene="ykgF"
                     /locus_tag="N7B39_003980"
     CDS             complement(5781..7208)
                     /gene="ykgF"
                     /locus_tag="N7B39_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HGU2423865.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGLWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDNVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(7219..7938)
                     /gene="ykgE"
                     /locus_tag="N7B39_003981"
     CDS             complement(7219..7938)
                     /gene="ykgE"
                     /locus_tag="N7B39_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HGU2423866.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVAHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         2190 a         1871 c         1787 g         2137 t
ORIGIN      
        1 tattaatgag ataatgagat tacatcgata acgttctgat tgcaggcata cttatctggg
       61 tgtgcctctc ttttttacaa ctgtacacgg tggcgtatga cgagaaaata tctcacacag
      121 gatgaagtct acaggctgat ggatgcagcc cagagcatgt cctttcctga aagaaatcgc
      181 tgtctaatta tgatggcttt cattcacggt tttagggcca gtgaacttct tgatttacgt
      241 ttatccgata ttgatgcatc aggaaaacaa cttaatattc ggcggattaa aaatggtttc
      301 tcgacaacac atcctcttct tcctgatgaa tataatttga tcaagttgtg gcttaagcag
      361 aggaagttaa ttgaaaacgg agttgaagga gactggcttt ttctctcccg gaaacgccgc
      421 cctatcagca gacaacattt ttttctatca ttcgtgaggc tggaagacgt gcaggattag
      481 ctgtaaaagc acatcctcat atgttacgtc atgcctgtgg ttttgctctg gctgacaatg
      541 gtgtcgatac ccgactactt caggattatc tgggtcatcg aaatatccag catactgtca
      601 gatacacagc cagtaatgct gctcgattta aaggggtgtg gaaaaaaaag cctcgttaaa
      661 acagtacctt ctgattcaaa atgtcagatt cctacgtttt cctcaattct tccataaaaa
      721 aacagcccat tcaattcaat aaaacacata acatgttgat atttctcgct aaagtaaaat
      781 aaccccatga tttgaaatga ttaaccgcca aggtcatacc agtgattaac tttcactcat
      841 gttgttaaat gcaaccaaat taaaaacaat aaaaatcaaa caaattagat ttatattcca
      901 agaaaattaa atattcaggc aattaaacaa aacctgacat tttgaatcaa aatgtcaggt
      961 tttgtagcta tggcgtggac gagtctgaat gaagaagcct ctggttatta tcagtgcctg
     1021 tcagtttacc aggctggcac tggagtctct catcccagcg gacaggtata tcgtgcgggt
     1081 gtacagcaac gtcactacag aggttgaatt cgttttaaag acctcctgtg gctatctgtt
     1141 ggctgatatc ccttagcttc cttcggggga gttagttctt ctggcttgtc ttgtccgctt
     1201 gcggagtgat gccgggcaat ggcaggtctg cctgaccggt gactccgact ggttcgataa
     1261 tgctcctgct gcaagtctgt ataacggctt cccacgcatc ggcaccatgc tgacagcgag
     1321 tcgttatcag aatcaaatta tccgctggtt actacgtcct ttgcccggcg atatgagcga
     1381 tttctggctc ctgactgcac gggagttgag cgtgcttaag attctgatgc agggcatgag
     1441 tttcagtgag attgcccgtt atgaacaacg cagttcaaag accctgcatg cgattgccac
     1501 acgggcatta atgaaacttg gactgggaac tctgagcgat ttcaggctgc tctatacggg
     1561 atgcggtgac agcagagtga aacggaatat acggctacat gcaagatatg tcggacaaca
     1621 ttccgctcat cgactcactc agtatgtgca gaacatggta tccggaattc tttatccaga
     1681 gaaagttgcc gttggtgaat caataaattc tgacgccatt tccggaaaag tacttcccgg
     1741 acaaagaaaa aatatttcta aaaaaagacc aggattgtta tgacacaaca gcgttgttac
     1801 taccccccac tgcggcgcgt acggactcgc tacagtgtag gggccaaaat atttctactg
     1861 ctctgggctg caggattggg aggcacgctg tacagcctgt acagcgtgct ggcgtactga
     1921 catctgtgag ggaaggatgc gtttcgttct tttctgtccc tcaggaattg taccagcgca
     1981 gttcgcagct ctatcaaccg gttctgtcgg caatgttacc tgcatcggaa cagaagagga
     2041 gttacgcaat aaattgcggc acaggccaca gagtgtcgtg atatcggcag ggcgacctgc
     2101 cgaatgcgct gaaatgtggt tccgatttta tcgggatcac tcattcgtgg tggtgttgtg
     2161 cgtcgcgcca ttttttctgc caccggatgt gagtatttct ggcgttctca aaaacctgag
     2221 gttactgaaa ccaggaatgt cagtagagca tgtcatcagt attgcaaatt cctctggtgg
     2281 gttatcaggg ctgaaacatg cagaaaattt gccagttatg gacagttact ctgtctttat
     2341 gaaagaggtg aataaccgca caaagacaat cgtcatgtca gaacgcttcc cggaaaagca
     2401 aaagaaggtg ttgtcgttgc ttcttgccgg acacagctgg gaatacagtg cgcagtttct
     2461 caaaacgggg ataagacaga tatggcttgc cgagcagtcg ctaaaaaagc gttggggaat
     2521 accggacagc atgtcattgc gggaagccct gctgctgagc agcaataaat ttcacggtga
     2581 tggaaatgca ttggaaaaaa cgaatgccat gacattgcgt gaaaacggaa ataccaacag
     2641 gtattccgtg gtggtaaatg cagggacgca ggttttatct ctgcataaat ataaataagt
     2701 gggaaattga aattagttac cagaatggag aatttcttca tgaaaaacag taaggcattt
     2761 taccgcagcg cattagcgac agctattgtt atggctcttt ctgcaccagc attcgctgct
     2821 gataacgcgg tatcaactga ttcggttacg ctgaataaag ataagacgac tctggatcaa
     2881 gatgttgtta ttagcaatac agcagacaaa cagattacag ccgtaacaat taatgcggca
     2941 gatgaagacc ttaatgttgc ttttgctggt catgatatta ctgctgaatc aacggcagac
     3001 aaaaaattcg ttgaaggtgt aaaagttagt ggtaacaaaa atgttgtgat taatgctaca
     3061 gactccacca tcacagctca aggtgaaggc acctatgtcc ggactgcaat ggtcattgat
     3121 tcaactggcg atgttgttgt taatggcggt aatttcgttg caaaaaatga aaaaggtagt
     3181 gcgacaggga tatctctgga aggggccaag ggaaataatg tcacgcttaa tggcacaacc
     3241 ataaatgctc aaggtaataa gagttccagc aacgcctcca cggcaatttt tgctcaaaag
     3301 ggtagcttat tgaatagttt tgaaggtgat gcaaccgaca acattaccct tgctggctca
     3361 aatattatta atggtcagat tgaaacaata gttactgccg ggaataagac gggaattcat
     3421 acagtcaacc tgaatattaa ggatggctca gtaattgggg cggctaataa taaacaaaca
     3481 atttatgcct ctgcttcggc acaaggcaca ggttcagcaa cgcaaaattt aaatttgtct
     3541 gttgctgatt caaccatcta ctctgatgtc ctggcccttt ctgaaagcga gaattcagcc
     3601 gctaccacaa caaatgtaaa catgaacgtt gcccgctctt actgggaagg taatgcttat
     3661 actttcaata gcggcgataa agcgggtagt aatctggata taaatctttc cgatagttca
     3721 gtctggaaag gcaaagtttc aggggcagga aatgccagtg tatctctgca aaacgagtct
     3781 gtctggaatg ttacgggatc ctcaactgtt gatgctctgg ctccgtctgg gtgatgagtc
     3841 tgtcaatgcc agcattgacg gtgcagcatt ccgcgtgggt gcaggtgtac aggctgatat
     3901 caccaaaaac atgggagcat atgcaagcct tgactacacc aaaggtgacg acattgagaa
     3961 tccgctacag ggtgtagttg gtatcaatgt gacctggtaa gaataaagca gtcggttccc
     4021 ctgtcgactg ttataccccc tgcaggcagc agtatgctgc ctgcaggcta taaatatcca
     4081 gtattcatga tgctggcttt ttgctgatat ttaacccgaa cattcctgat atagactatt
     4141 aattcattta tctgcggcag attttatttt ctggatatat tatgagtgaa caaatcaaac
     4201 aggatatcga tctgattgag attttatttt atctgaagaa aaagattctt gttatccttt
     4261 ttattatagc tatctgtatg gcaatggtgc ttttatttct gtatatcaat aaagataata
     4321 taaaagtgag ttacagccta aaaataaatc agacaacacc cggcatactt gttaactgtg
     4381 atagcaataa taactttgcc tgtcagacaa caatgactga agatgttatt cagcgaatta
     4441 ctacattttt tcacaccagc ccagatgtca agaacagaga aataaagctg gaatggtcag
     4501 gagataagag agatttacca actgctgaag cggaaatatc tcgcgtgcag gcgtctatta
     4561 tcaaatggta tgcgtcagaa taccataatg gcaggtaagt tctcgatgag atacaaacgc
     4621 cttcagcaat taacagtgag ctttatacaa aaatgatata cctgaccagg aactggtcat
     4681 tgtatccgaa cggtgatggc tgtgtaacta taagctcacc agaaataaaa aataaatacc
     4741 ctgctgccat ttgcctggct ctgggatttt ttctaagcat tgtaatttct gtaatgtttt
     4801 gtcttgtcaa aaaaatggta gatgaatacc aacaaaactc tgggcagtaa taaacagcat
     4861 tctgatagag agtgcctgta taaagctgaa gtgacaatga ccgggaaggg aacccgtttt
     4921 caggaatgga tgcctgtctt tttcactgta aatatcctgg cattagtaat gccaggatat
     4981 ttacagaaga ggagcataac catgacggaa cggggttaag tcaggttgtc cttaacgcta
     5041 ttttcaacct cgtatgcctt cttcaggttt atgtccagac ttcatacctc tctcagcaat
     5101 cctcaataat cagatacacc gctttcaccg gaccatgaac cccgacgact ttgataagct
     5161 caatgtccgc cgttgagctg ggaccactga tgatgttaat acatgacggc atgcgttcac
     5221 cggcctgcgc tttctgatgc aatctttctg cgagttgcgc tacacgcggc aggatagtgc
     5281 ttttacgcag gataaaaaga gatgattccg ggagcaggct caatgaacgc ccacgctcgg
     5341 cggcggaaaa aagaaccacg cctcccgatt cggttaaacc atattcagca tacacgacac
     5401 ccactttagc ctgctctgcc tgcgagatat tcttggcacc tttcgccgga tcccaaacaa
     5461 cggcattgca ttcctgctgc aaacgttcgc taatccctaa ttcctccagc ctcgtgtcac
     5521 cgctaatcac gaccgactga tctcccagct ctttacacag acgtattgca gcttctgccg
     5581 ccttcgcctc gctggtcagc tcacagcgcg tcaacataac atcgctggca aactgaataa
     5641 acgcgtcaca gcgctgctgt tggttaagtt gggtaagccg ctcgttagca tagttattaa
     5701 gcggcgcatc ttctgcttgc ggttcaagtc gcagcgggcg acccagtgcc tgagcaacgt
     5761 tattcaaaaa ttcgcctcga ttatccattc tttttctcct gcgcctgatg tttcttaaac
     5821 caactacgga aactctctcc gtcagcttca ggaagatcgc gtgcttccat ccagtcgcta
     5881 atcgcgccaa atttgagtgg tgttttgccg ccattgataa accagcttgc cgcatgagca
     5941 ccggccatca tcccgacttt ccacaatcct ggatgactat tggcataagc gaacattttt
     6001 atcgcccgtt gctctgcttt tgcggtgatc cctttttcag ccatcacccg acgatgacgc
     6061 aaaatcagtt ttgacagcgg aatacgcacc ggacacacgt tgtcacaagc tgtgcataaa
     6121 gagcaggcgt agggtaaatc tttaaaatct ttatagccgc caagtagcgg agaaatcacc
     6181 gcaccaattg gccctggata aatagagcca tatccatgac cgccaatatg gcgatatgcc
     6241 ggacaagtat tcatacaagc cccgcagcga atacagcgca gcacatcccg aaattcagag
     6301 gccagcacct cagaacgccc gttatcgaca ataaccagat gaaactcttc aggaccatca
     6361 acgtgccccg cttcgcgagg tcctgtcagc caggtgttgt atcccgtcaa acgtgcacca
     6421 acggcactgc gcgccagcat ggtgatcaat acatctacct cggcaaacgt gggggcaata
     6481 cgctccattc ccatcactgc aatatgcgtt ttaggcagcg tggtacacat tcgcgcatta
     6541 ccttcattgg tcaccaggca taccgaacct gtctctgcca ccgcgaaatt acagcccgta
     6601 atacctattt cagcactgag gaaatcttcg cggatttttt gccggatgaa taaggtcatc
     6661 gcttcaggcg tttccggccc ctcatagccc agacgttcgt gtagcactcg acggatctga
     6721 tggcgatctt tatgaattgc cgggaccaca acatgagagg gcggatcttg atccagctgg
     6781 agaatatatt cacccaaatc ggtttcaatc acctgaatgc cagcatcctg caacacatga
     6841 ttgacaccaa tctcttcggt caccatcgat ttagatttca ccaccttccg ggcatttttg
     6901 cgttgggcaa cctgtaaaat gtagcgggta gcgtcttctt tggtttttgc aaaatagacg
     6961 tgaccgccgt tttgcgtcac tttttctgag agctggtaca gataagcgtc gagattactc
     7021 agaacatgat cacgtatctg ggcggcccga tcgcgccact cctcccagag ccccaattca
     7081 tcgaccattt tttgccgatt tgccccaata cgctgctgcg cgtttgccac cgctttgcgc
     7141 atgatcggat cttcaatttg ctgacggatg cgtgtcttaa aatctgtatt actggttttg
     7201 atcgacatct ttatatcctc agcggctcat caacacttca gcaatatgca tcactttgac
     7261 tttctgccct tcccgttgta atcgcccact gatgttcagc aggcaactca cgtcagcacc
     7321 aattaaatac tcagggcgga cttccatcag gtgcgcaacc ttttctttca ccatctcgcc
     7381 ggatatttcg gccattttga ccgagaacgt gccgccaaat ccgcagcagg tatcctgttc
     7441 agcaaaggtc aacagctcca gtccacgcac atttttcagc agcgtaagtg gctcgtcctt
     7501 cacccccagc ttacgggcca ggctacaaga tgggtgatac accgctctcc cttgcaaact
     7561 ggcacctaca tcgactaccc ctaatttatt aacaataaaa gaggtgagat cctgcatacg
     7621 cgcggcaacc ttttcggctc gtgatgccca ttcaggttca tccgccagat acgtcgggta
     7681 actttttacg gcataggtgc aagagccagc cggtgaaata atgggatcgt cgttatcctc
     7741 cagtgcggcg atcagatttt tcatccctgg aatcgcttct ttgatataac cactattgat
     7801 cgcaggctga ccgcagcatc cctgtttctc cgggaaattt acgcgacagc cgagtttttc
     7861 cagtagcagc acggagtctc gtgccattct tgatttcagg gcgtcaccaa tacaggtgac
     7921 aaagaaattg acattcacaa ctaatactcc attacttcat gcccatttat tcccgatgtt
     7981 tcccc
//
LOCUS       DBFIFI010000197         7933 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.197, whole genome shotgun sequence.
ACCESSION   DBFIFI010000197 DBFIFI010000000
VERSION     DBFIFI010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7933
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.197"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..1054)
                     /locus_tag="N7B39_003982"
     CDS             complement(<1..1054)
                     /locus_tag="N7B39_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU2423867.1"
                     /translation="MAYYNIEKRLKSDGTPRYRCNVIIKEKGVITYRESKTFPKHAHA
                     KTWGTQKVMELDLYGIPSSNAVDGLTVRDLLHKYLNDPNAGGKAGRTKRYVLELLMDS
                     DISAIKLSELTENDVIEHCRLRNNAGAGPATVSHDVSYLGSVLDAAKPVYGINYTSNP
                     AKSARPYLLKLGLIGKSNRRNRRPASDELDMLIEGLQQRSTHKCSKIPFVDILKFSVW
                     SCMRIGEVCRLRWEDLDQEQKSILVRDRKDPRKKEGNHMKVALLGEAWDIVQRQPKKS
                     EFIFPYNSTSVTAGFQRVRSKLGIKDLRYHDLRREGASRLFEAGFSIEEVAQVTGHRS
                     LNVLWQVYTELYPKSLH"
     gene            1143..2180
                     /gene="dusA"
                     /locus_tag="N7B39_003983"
     CDS             1143..2180
                     /gene="dusA"
                     /locus_tag="N7B39_003983"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HGU2423868.1"
                     /translation="MHDNHEAQKINQTSVMPEKTGVYWNSRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     LHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            2314..2556
                     /gene="pspG"
                     /locus_tag="N7B39_003984"
     CDS             2314..2556
                     /gene="pspG"
                     /locus_tag="N7B39_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HGU2423869.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(2722..3705)
                     /locus_tag="N7B39_003985"
     CDS             complement(2722..3705)
                     /locus_tag="N7B39_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HGU2423870.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            3788..5203
                     /gene="dnaB"
                     /locus_tag="N7B39_003986"
     CDS             3788..5203
                     /gene="dnaB"
                     /locus_tag="N7B39_003986"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HGU2423871.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISSYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            5256..6335
                     /gene="alr"
                     /locus_tag="N7B39_003987"
     CDS             5256..6335
                     /gene="alr"
                     /locus_tag="N7B39_003987"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HGU2423872.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKVGEPVGYGGTWISERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            6588..7781
                     /gene="tyrB"
                     /locus_tag="N7B39_003988"
     CDS             6588..7781
                     /gene="tyrB"
                     /locus_tag="N7B39_003988"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HGU2423873.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAVPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLAMLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
BASE COUNT         1872 a         1933 c         2062 g         2066 t
ORIGIN      
        1 tatgtaaaga tttcggatac agttcggtat atacctgcca tagcacgttt aatgaacgat
       61 gccctgtaac ttgagcgact tcctcaatac taaaaccagc ctcaaataag cgacttgccc
      121 cttctctacg caaatcatga tatcgcagat ctttaatacc taatttgctt cttaccctct
      181 gaaatcctgc ggtaacagaa gtgctgttat atggaaaaat gaattctgat ttttttggtt
      241 gtcgctggac gatatcccag gcttccccaa gcaaggcaac tttcatatgg ttaccttcct
      301 ttttacgtgg atctttccta tctcttacta gtatagattt ttgttcttgg tcgagatctt
      361 cccatcgtaa ccggcatact tctccgattc gcatacagga ccacacagaa aatttgagga
      421 tatcaacgaa cggaattttt gagcatttat gagtagatcg ttgttgaagg ccttcaatga
      481 gcatgtcaag ttcatcagat gctggtctac gattacgacg gtttgattta ccaatcaaac
      541 cgagtttaag tagatatgga cgagcgcttt tcgccgggtt tgatgtgtaa ttgattccgt
      601 atacaggttt tgccgcatcc agaacactgc caagataact aacatcgtgg ctgactgttg
      661 ctgggcctgc accagcgttg tttcttagcc tgcaatgttc aattacgtca ttttctgtca
      721 gttcagatag tttgatcgcg gatatgtcac tatccataag cagttccagc acatatcttt
      781 tagtacggcc tgctttacct ccggcatttg ggtcatttaa atatttgtgt agtaagtcac
      841 ggactgtaag tccgtcaact gcatttgatg atggaatgcc atatagatct aattccatca
      901 ctttctgtgt gccccatgtt ttggcatgag catgtttagg gaatgttttg ctttccctgt
      961 aagtgataac acctttttct ttgataatca cattacagcg atagcgtggt gtgccatcgg
     1021 attttagtcg tttctctatg ttatagtacg ccattacacg acctcgttat ttcgggttcc
     1081 cataaaacgt gggaacctgt gcgggaacct aacgcgagaa aaatagcctg aaatgttcaa
     1141 aaatgcacga taatcatgaa gcacaaaaaa ttaatcaaac cagcgtgatg cctgaaaaaa
     1201 ctggtgttta ctggaattct cggtttagcg ttgcaccaat gctcgactgg acggacagac
     1261 attgccgcta tttcttgcgt ctgctttccc gcaatacgtt gctgtatacc gaaatggtga
     1321 ctacaggggc gattattcac ggtaaaggtg attacctggc gtacagtgaa gaagaacatc
     1381 cggtagcgtt gcaactcggc ggtagcgatc cggcggcgct ggcgcagtgt gcgaagctgg
     1441 cagaagcgcg tggatatgat gagatcaacc tgaatgtcgg ctgcccgtct gaccgggtgc
     1501 agaacggcat gtttggtgcg tgtctgatgg gtaatgcgca gctggttgcc gactgcgtga
     1561 aagcgatgcg cgatgtggtg tcgattccgg tgacggtgaa aacgcgtatt ggcatcgatg
     1621 accaggacag ctatgaattt ctctgcgatt tcatcaacac cgtttccggc aaaggcgagt
     1681 gtgagatgtt catcatccac gcacgtaaag cctggctttc ggggttaagc ccgaaagaaa
     1741 accgtgaaat cccgccgctc gattatccgc gtgtgtatca actgaagcgt gactttctgc
     1801 atctgacgat gtcgattaac ggtggtatca agtcgctgga agaggctaaa gcgcatttgc
     1861 aacatatgga tggcgtgatg gtcgggcgcg aagcgtatca gaatccgggt attctggcgg
     1921 cggtagaccg agagatcttt ggttcctcgg ataccgatgc cgatccggtg gcggtagtgc
     1981 gcgccatgta tccgtacatt gagcgtgaac tcagccaggg gacgtatctt ggccatatta
     2041 cccggcatat gttgggcttg ttccagggta ttcctggcgc gcggcagtgg cggcgttatt
     2101 taagtgaaaa tgcccataaa gcgggtgcag acattaacgt gctggaacac gcgctcaaac
     2161 tggtggcgga taagcgttaa cttttcacca aaaagtagtc aaattcacca cgccctgcgc
     2221 accgtcgcgg ggcgttttgc tgttaaatca atagattatt tttggcatga ttcttgtaat
     2281 gcctgcaaga gatttcatat ttgggagagc atcatgctgg aactactttt tgtgattggc
     2341 ttttttgtca tgttgatggt caccggcgtt tcgttgctgg gcattatcgc cgcgctggtt
     2401 gtggcgacgg ccattatgtt cctcggcggt atgctggcat tgatgattaa gttgctgccg
     2461 tggttactcc tggcgattgc ggtggtgtgg gttattaagg cgattaaagc accaaaagtg
     2521 ccgaaatatc agcgttatga ccgctggcgt tactaaggga ttgtgcggat gatcacaacc
     2581 taaggtttta tccttagaac aaaataggaa ttgataatca aatctgtcac tattgcgcct
     2641 ctaacagatt catcgtgctg taccctacat acagccgaac tataaaaaga aagggcttcc
     2701 caggtggaag ccctatttct tttatggaat caacaggctg gaaccttgcg tcgcgcggct
     2761 ttccagaatc tcatgcgcac gctgcgcatc cttcagcgga tatttctgct gctcggcgac
     2821 atcgacctta atcacaccgc tggcaatcaa agagaacagt tcattactgg cctcggttaa
     2881 ttcctcccgc gtggtgatat agccttgcag ggaagggcgt gtcacataca acgagccttt
     2941 ctgattgaga atgcctaagt tcacaccggt aaccgcacct gatgagttgc caaaactcac
     3001 cattaagccg cggcgttgca ggcaatccag cgaccgttcc caggtgtctc tgcccacgga
     3061 atcgtacact acgcgcactt ttttaccacc ggtgatctct tttaaccgct cgaccagatt
     3121 ctcttcacga tagttaataa cctgccacgc gcccgctttt agcgcgctct gcgctttttg
     3181 cgcggttcct acggtgccga taagtttcgc gcccagggct tttgcccact ggcaggcaat
     3241 caagccaacg ccgccagctg ctgcgtggaa caggaactgt tcatcgggtt taatttcata
     3301 ggttttgcgc agcagataat aaaccgttaa gcctttcagg aaagatgctg cagcttgctc
     3361 aaaagaaatt gccgcaggca gaatcgccgc tttatccgca ttaatgttat gcacagagct
     3421 gtaagcgcct aacgccgact gcgcatagac tacacgatcg cctgccttaa tatgctttac
     3481 accactgccg actttactca cgatgcctgc cgcttcggta cctaatccgc tgggtagtga
     3541 tggcggcggg taaaggccgc tgcggatata cgtgtcgata aaattgatgc cgatggcttt
     3601 attttcgacc tggatttcat tctccgccgg atcggcagga gtgaactcta cggcttgaag
     3661 tacttccggg ccaccgtgct tgtgaaattc aattcgtgtt gccatgtgtc ctccagaacg
     3721 tatcgtcagg gtctgcttca tatggtaaag tttcgaccca ttctttatct cggtaactcc
     3781 attcactatg gcaggaaata aacccttcaa caaacagcag gctgaacccc gcgaacgcga
     3841 tccacaagtt gccgggctga aagtgcctcc gcactcgatc gaagcggagc agtcggtgtt
     3901 gggcggttta atgctggata acgaacgctg ggatgatgta gccgagcgtg tggtagcaga
     3961 cgatttttac acccgcccac accgtcatat ctttactgaa atggcgcgtt tgcaggaaag
     4021 cggtagtcct atcgatctga ttacccttgc ggaatcgctg gaacgccagg ggcaactcga
     4081 tagcgtcggt ggttttgctt atctggcaga gttgtcaaaa aatacgccaa gtgctgcgaa
     4141 catcagttcc tatgcggaca tcgtgcgtga acgtgccgtt gtccgtgaga tgatctcggt
     4201 tgcgaatgag attgccgaag ctggttttga tccgcagggg cgtaccagcg aagatctgct
     4261 ggatctggct gaatcccgcg tctttaaaat tgccgaaagt cgtgcgaaca aagacgaagg
     4321 gccgaagaac atcgccgatg tgctcgacgc aaccgtggcg cgtattgagc agttatttca
     4381 gcagccacac gatggcgtta ccggggtaaa caccggttat gatgatctca acaaaaaaac
     4441 cgctggcttg cagccgtcgg atttgatcat cgtcgccgcg cgtccgtcga tgggtaaaac
     4501 aacatttgcg atgaacctcg tcgaaaacgc ggcgatgttg caggataaac cggtacttat
     4561 tttctcgctg gagatgccat cagaacagat catgatgcgt tctctggcgt cgctgtcgcg
     4621 cgttgaccag actaaaatcc gtaccgggca gctcgatgat gaagactggg cgcgcatttc
     4681 cggcaccatg ggtattttgc tcgaaaaacg caatatctat atcgatgact cctccggcct
     4741 gacgccaacg gaagtgcgtt cccgcgcacg ccgtattgcc cgtgaacacg gcggcatcgg
     4801 gcttatcatg atcgactacc tgcaactgat gcgcgtaccg gcactttccg ataaccgtac
     4861 gctggaaatt gcagaaatct cccgctcgct gaaagcactg gcgaaagaac tgaacgtgcc
     4921 ggtggtggcg ctgtcccagt tgaaccgttc tctggaacaa cgtgccgaca aacgtccggt
     4981 caactccgac ctgcgtgaat ctggctctat cgagcaggat gcggacttga tcatgtttat
     5041 ctatcgtgat gaggtgtatc acgaaaacag tgatttaaaa ggcatcgcgg aaattattat
     5101 cggtaaacaa cgtaacggcc caatcgggac ggtacgcctg acctttaacg gtcaatggtc
     5161 gcgcttcgac aactatgcgg ggccgcagta cgacgacgaa taataattat tttatgaatt
     5221 aggtaattaa agcaaacact tatcaaggaa cacaaatgca agcggcaact gttgtgatta
     5281 accgccgcgc tctgcgacac aacctgcaac gtcttcgtga actggcacct gccagtaaaa
     5341 tggttgcggt ggtgaaagcg aacgcttatg gtcacggtct tcttgagacc gcgcgaacgc
     5401 tccccgatgc tgacgccttt ggcgtagccc gtctcgaaga agctctgcga ctgcgtgcgg
     5461 ggggaatcac caaacctgta ctgttactcg aaggcttttt tgatgccaga gatctgccga
     5521 cgatttctgc gcaacatttt cataccgccg tgcataacga agaacagctg gctgcgctgg
     5581 aagaggctag cctggacgag ccggttaccg tctggatgaa actcgatacc ggtatgcacc
     5641 gtctgggcgt aaggccggaa caggctgagg cgttttatca tcgcctgacc cagtgcaaaa
     5701 acgttcggca gccggtgaat atcgtcagcc attttgcgcg cgcggatgaa ccaaaatgtg
     5761 gcgcaaccga gaaacaactc gctatcttta ataccttttg cgaaggcaaa ccaggtcaac
     5821 gttccattgc cgcgtcgggt ggcattctgc tgtggccaca gtcgcatttt gactgggtgc
     5881 gcccgggcat cattctttat ggcgtctcgc cgctggaaga tcgctccacc ggtgccgatt
     5941 ttggctgtca gccagtgatg tcactaacct ccagcctgat tgccgtgcgt gagcataaag
     6001 tcggagagcc tgtcggttat ggtggaacct ggataagcga acgtgatact cgtcttggtg
     6061 tagttgcgat gggctatggc gatggttatc cgcgcgccgc gccgtccggt acgccagtgc
     6121 tggtgaacgg tcgcgaagtg ccgattgtcg ggcgcgtggc gatggatatg atctgcgtag
     6181 acttaggtcc acaggcacag gataaagccg gggacccggt cattttgtgg ggtgaaggtt
     6241 tgcccgtaga acgtatcgct gaaatgacga aagtaagcgc ttacgaactt attacgcgcc
     6301 tgacttcaag ggtcgcgatg aaatacgtgg attaatcgtt ctgtaatatt tgattgtctg
     6361 tgccggatgc ggcgtgaatg ccttatccgg ccaataaaat cctaccaatt caataagttg
     6421 atattctttc atgctcttat aaaggtcgtg cctctggcgg atgtacgttt gtcatgagtc
     6481 tcactctgtt gctaattgcc gttcgcccct gaacatccac tcgatcttcg ccttcttccg
     6541 gtttattgtg ttttaaccac ctgcccgtaa acctagagaa ccatcgcgtg tttcaaaaag
     6601 ttgacgccta cgctggcgac ccgattctta cgcttatgga gcgttttaaa gaagaccctc
     6661 gcagcgacaa agtgaattta agtatcggtc tgtactacaa cgaagacgga attattccac
     6721 aattgaaagc cgtggcggag gcggaagcgc gcctgaatgc ggtgcctcat ggcgcttcgc
     6781 tttatttacc gatggaaggg cttaacagct atcgccatgc cattgcgccg ctgctgtttg
     6841 gtgccgacca tccggtactg caacaacagc gcgtagcaac cattcaaacc cttggcggct
     6901 caggggcatt gaaagtgggc gcggatttcc tgaaacgcta cttcccggaa tcaggcgtct
     6961 gggtcagcga tcctacctgg gaaaaccacg tagcaatatt cgccggggct ggattcgaag
     7021 taagcactta cccctggtat gacgaagcga ctaacggcgt gcgctttaat gacctgttgg
     7081 cgatgctgaa aacattacct gcccgcagta ttgtgttgct gcatccatgt tgccacaacc
     7141 caacgggtgc cgatctcact aatgaccagt gggatgcggt gattgaaatt ctcaaagccc
     7201 gcgagcttat cccattcctt gatattgcct atcaaggatt tggtgccggt atggaagagg
     7261 atgcctacgc cattcgcgcc attgccagcg ctggattacc cgctctggtg agcaattcgt
     7321 tctcgaaaat tttctccctt tacggcgagc gcgtcggcgg actttctgtt ctgtgtgaag
     7381 atgccgaagc tgcaggccgc gtactggggc aattgaaagc aacagttcgc cgcaactact
     7441 ccagcccgcc gaattttggt gcgcaggtgg tggctgcagt gctgaatgac gaggcattga
     7501 aagccagctg gctggcggaa gtagaagaga tgcgtactcg cattctggca atgcgtcagg
     7561 aactggtgaa ggtattaagc acagagatgc cagaacgcaa tttcgattat ctgcttaatc
     7621 agcgcggcat gttcagttat accggtttaa gtgccgctca ggttgaccga ctacgtgaag
     7681 aatttggtgt ctatctcatc gccagcggtc gcatgtgtgt cgccgggtta aatacggcaa
     7741 atgtgcaacg tgtggcaaag gcgtttgctg cggtgatgta atgcaggaaa gcaggctgga
     7801 attacccagc ctgcagtgaa attaaactgt cgtcgctttc actctttctt tatagatgat
     7861 ttttttgata ccatcgttct acgtgagaga taataaacgt tgttagttct tttattgtta
     7921 agtttatccc aat
//
LOCUS       DBFIFI010000198         7923 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.198, whole genome shotgun sequence.
ACCESSION   DBFIFI010000198 DBFIFI010000000
VERSION     DBFIFI010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7923
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.198"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            1..1104
                     /gene="ompN"
                     /locus_tag="N7B39_003989"
                     /pseudo
     CDS             1..1104
                     /gene="ompN"
                     /locus_tag="N7B39_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="porin OmpN"
     gene            1245..1679
                     /gene="uspF"
                     /locus_tag="N7B39_003990"
     CDS             1245..1679
                     /gene="uspF"
                     /locus_tag="N7B39_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HGU2423874.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            1855..2790
                     /gene="ttcA"
                     /locus_tag="N7B39_003991"
     CDS             1855..2790
                     /gene="ttcA"
                     /locus_tag="N7B39_003991"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HGU2423875.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(2919..4292)
                     /gene="dbpA"
                     /locus_tag="N7B39_003992"
     CDS             complement(2919..4292)
                     /gene="dbpA"
                     /locus_tag="N7B39_003992"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HGU2423876.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(4325..4495)
                     /gene="ynaL"
                     /locus_tag="N7B39_003993"
     CDS             complement(4325..4495)
                     /gene="ynaL"
                     /locus_tag="N7B39_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HGU2423877.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRA"
     gene            complement(4770..5753)
                     /gene="zntB"
                     /locus_tag="N7B39_003994"
     CDS             complement(4770..5753)
                     /gene="zntB"
                     /locus_tag="N7B39_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HGU2423878.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(5767..5814)
                     /gene="ydaN"
                     /locus_tag="N7B39_003995"
     CDS             complement(5767..5814)
                     /gene="ydaN"
                     /locus_tag="N7B39_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HGU2423879.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            6008..7240
                     /gene="dgcM"
                     /locus_tag="N7B39_003996"
     CDS             6008..7240
                     /gene="dgcM"
                     /locus_tag="N7B39_003996"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HGU2423880.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFIHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(7261..7824)
                     /gene="smrA"
                     /locus_tag="N7B39_003997"
     CDS             complement(7261..7824)
                     /gene="smrA"
                     /locus_tag="N7B39_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HGU2423881.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
BASE COUNT         2084 a         1997 c         1874 g         1968 t
ORIGIN      
        1 aagtactggc acttttaatt cctgccctgc tcggcgcagg tgctgcacat gcagccgaag
       61 tttataataa agacggcaac aaattagatc tgtatggcaa agttgatggc ctgcattatt
      121 tttctgataa ttcagcgaaa gatggcgacc agagctatgc gcgtctgggt tttaaaggcg
      181 aaacccaaat taacgatcaa cttactggct acggtcaatg ggaatacaat attcaggcaa
      241 acaacactga atcttcaaaa aaccagtcat ggacccgtct ggcatttgcc ggactgaaat
      301 tttcagatta cggttctttc gattacggac gtaattatgg gctggaccga tatgctgcct
      361 gaatttggcg gtgactctta taccaatgca gacaacttta tgactggtcg tgccaatggc
      421 gtcgcgactt atcgtaatac tgatttcttc ggtctggtaa atggtctgaa cttcgcggtg
      481 cagtatcaag gtaacaacga aggtgccagt aatggtcagg aaggcaccaa caacggacgt
      541 gatgttcgcc atgaaaacgg tgacggctgg ggtctttcca caacatatga tttaggcatg
      601 ggctttagcg ctggtgcggc atacacctct tctgaccgca ccaatgacca ggttaaccat
      661 actgcggcgg gtggtgataa agcagacgcg tggactgctg ggctaaaata cgatgctaac
      721 aatatttacc tggcaaccat gtattcagaa acgcgtaata tgaccccgtt tggcgacagc
      781 gattatgctg ttgcaaacaa aacccagaac tttgaagtca ctgcacagta ccagtttgat
      841 tttggtctgc gtccggcagt ctctttcctg atgtctaaag gccgtgacct gcacgctgcc
      901 ggtggtgcag acaacccggc aggtgttgat gataaagatc tggttaaata cgccgatgtt
      961 ggcgcgactt actatttcaa taaaaacatg tccacttatg ttgactataa aatcaacctg
     1021 ttggatgaag atgacagttt ctacgctgcc aacggcatct ctaccgatga tattgtcgct
     1081 ttaggtctgg tttatcagtt ctaaatcctc ctgcccgctg ttatggcggg ctttttctgc
     1141 ttattcttcc tcctttgatc taaattaaaa atgtgaactc cgtcattaca caaaaagtgt
     1201 catctggcgt tacactttat gcggatacta aaacaggagg ttttatgaac agaacgattc
     1261 ttgtccctat cgatatttcc gattcagaat taactcaacg cgtgattagc cacgttgagg
     1321 cagaggcaaa gattgatgat gcagaggttc atttcctgac ggtaatacct tcactgccct
     1381 actatgcctc tctgggttta gcgtattccg cagaattacc ggcaatggat gacctgaaag
     1441 cggaagccaa atcgcaactg gaagagatca ttaaaaaatt taaactgcca accgacagag
     1501 tgcatgtcca tgttgaggaa ggctcgccca aagaccgcat tctggaattg gcgaagaaga
     1561 tccccgctca tatgatcatc attgcttccc atcgaccgga tatcaccact tatctgctcg
     1621 gttccaacgc cgcagctgta gtgcgtcacg cagagtgctc cgtgctggtt gtgcgctgac
     1681 actaacgccc gcacattgct gcgggctttt tgattcattt cgcaaatgtg ctgacatttt
     1741 cccctctaat ccgtaccata cacgccacag tttttatatc agatttctta tgctgggcgt
     1801 tccggcatga cgcatactct tctgatgcca tataacgaat tgagtcgctt ttaaatgtcg
     1861 caaaatcaag aaattagcaa gaaagaacaa tacaacctga acaaattaca aaaacgcctg
     1921 cgtcgtaacg tgggcgaagc cattgctgac ttcaatatga ttgaagaagg cgatcgcatc
     1981 atggtttgcc tctccggggg taaagacagc tataccatgc tggagattct gcgcaatttg
     2041 cagcaaagcg cgccaatcaa tttttcgctg gtggctgtta acctcgatca aaagcaaccg
     2101 ggcttcccgg aacacgttct gcccgagtat cttgaaaagc tgggcgttga gtacaagatt
     2161 gttgaagaga acacttacgg tatcgtgaaa gagaagattc cagagggcaa aaccacttgc
     2221 tcactgtgtt ctcggctacg tcgcggtatc ctttatcgca ccgcaacgga actgggggcg
     2281 acgaagatcg cgttgggcca ccatcgtgac gatatcctgc aaacgttgtt cttaaatatg
     2341 ttctacggcg gcaagatgaa aggtatgcct ccgaaactga tgagcgatga tggcaaacat
     2401 atcgttattc gtccgctggc ctactgccgc gagaaagata ttcagcgatt tgccgatgca
     2461 aaagcgttcc cgattattcc gtgcaacctg tgcggttcac agcctaacct gcaacgtcag
     2521 gtgattgctg acatgttgcg tgactgggat aaacgttatc cagggcgtat cgagacgatg
     2581 ttcagcgcga tgcagaatgt ggtgccgtcg catctgtgcg ataccaacct gttcgatttc
     2641 aaaggcatca cccacggttc tgaagtggtt aacgggggtg atctggcgtt tgatcgcgaa
     2701 gagatcccac tacaaccggc gggctggcag ccagaagaag atgaaaatca gttggatgag
     2761 ttacggctga atgtggttga agtgaaataa ctcggatagc gcccgatgcg caaacgtatc
     2821 gggctactct aatggaggct gaataagacg cggtcagcgt cgcatccgac aatcccgacg
     2881 aagatgttta cttttgcatc aggcaattca acattttatt attttaataa ccgcacccgg
     2941 cacgtttttc ctttaatctt cccgccctgt aactgtttcc atgctttatg agcaacagcc
     3001 tgacggaccg cgacatagac atgcgccgga tgcacggcga ttttgccaat atctgcgcca
     3061 tcaagcccga tatctcctgt cagtgcacct aatacatcac ccgggcgcat tttggctttt
     3121 ttcccgccat cgatacacaa cgttgccatt tctgcttcca gcgtcacaat ggaactatta
     3181 gctggtggcg tttgccagtt aagttttatc tgcaacatgt cagaaatgat attggcccgc
     3241 tgtgcttctt ccggagcaca gaaactgatc gccagaccgc tatttcctgc acgagctgta
     3301 cgaccgatgc gatgtacatg aacttcaggg tcccacgcca gctcaaagtt caccaccagc
     3361 tcaagcgatt taatatccag accacgcgca gcaacatcag tcgcgaccag tacacgggcg
     3421 ctaccgttag caaaacgtac cagggtctga tcacgatcgc gttgctccag atcgccgtgt
     3481 aatgacaatg cactttgccc tacttcattc agcgcgtcgc agacagcctg gcaatctttt
     3541 ttggtattgc aaaacaccac gcaagaggat ggctgatgca agcttaataa ccgttgcaac
     3601 agaggaattt tgcctttgct ggatgtctca taaaattgtt gttcaatggg tggcaaagca
     3661 tctgttgagt caatttcaat cgccaaagga tcgcgttgca ctcgtccgct gattgcggcg
     3721 atggcttccg gccaggttgc cgaaaacaga agcgtctgtc gagatgcagg cgcaaaacgg
     3781 atgacatcat caatggcatc gctaaatccc atatccagca tgcggtcggc ctcatccatc
     3841 accaaagtat tcagcgcatc cagtgatacc gtgccttttt gcaggtgatc cagcaaacgg
     3901 cccggcgttg ccacgataat atgcggcgca tgttgcaacg aatcacgctg cataccgaac
     3961 ggttgaccac cgcacaacgt caaaattttg gtatttggca gaaaacgcgc cagccgacgc
     4021 aattcacctg ccacctgatc cgccagttca cgcgtaggac acagcactaa agcctgggtt
     4081 tgaaatagcg acgcatcaat ttgctgtaac aagccgaggc caaaagccgc cgttttgccg
     4141 ctgccagttt tcgcctgcac gcgaacatct tttccggcaa ggatcgccgg aagcgcggcg
     4201 gcctgcaccg gcgtcatggt taaataaccc aactcattaa ggttcgtgag ttgggcggga
     4261 ggcaaaacat tcagggtaga aaaagcggtc acaatctatt ctcgtggtca tcgacgcaaa
     4321 gtcatcaggc gcgtatcctc gcagatctac gctcacgatg cgacaattta atcggttctt
     4381 catcgggtgg tgggtcaggc atgggttgcg ggcgagggat cggatcgggc actggaacag
     4441 gatcgccagg aatcggttca gggacaggaa tttgcaaata aataagtgtc gtcatatttc
     4501 cctccggtca ttgggtggac tcttaaaggg tagacgctga taaataacag gcaaaaaaaa
     4561 gccgactcat caaagtcggc gtcgtacgaa tcaattgtgc tatgcagtaa ttcaaaaaag
     4621 gaagtaagac aatatggagc gcaacgccca tcgcttgacg ttgcattcac ctgcaagaga
     4681 gatattgccc tgaatgggta gagagtttat tgacttcgct caaactttgc ggcgtttttg
     4741 tatacagaca gccggaaaaa ttgcttttgt tacaaccatt tactacgatg caaccataaa
     4801 gcaacaccac caataagaac aactaacaga atacaaaaaa ttgaaaatcc gaattgccac
     4861 ccgccgccag ggatcccacc aaggttgacg ccaaataacc ctgtcagaaa ggtactgggt
     4921 aaaaagacca ttgccatcaa tgacattgta taggtacgac gagctaaatt ttcctgcatc
     4981 acctgagcga tttcatccgc catcacacca gtccgtgcta tacaggcgtc gatttcgtca
     5041 aggccgcgcc caaggcgatc ggcaatatcc tgcatccgac ggcgttggtc atcgctcatc
     5101 cacggcaaac gttcactggc aagacgagca taaacatcac gttgcggtgc catatagcga
     5161 cgcatcacaa ttaattgttt gcgcagcaga gccaggaatc cacgcggcgg aatttgctga
     5221 tcaaggagat tatcttcaag gtcgataatt ttatcgtgca gctgctcgat aaattcgctg
     5281 gaatgatcgg tcaacgcatc gcacacatcc accagccatc ccccgcaatc ggtcggaccc
     5341 gtgccctctt ccagatcact caccacatcg tccagcgcca gcactttgcg ttgtcgggtc
     5401 gaaacaatta accgcccgtc catatataca cgcatggcga ccagttgatc ggggcgttca
     5461 tcggtgctgc cgtttataca gcgcaatgta atcagcgtgc cttcaccgag acggctgact
     5521 cggggccgtg tgctctcgcc cgccagcgca tcacgtacgt tattgggaag cagcggtgtt
     5581 gtcgccagcc attgggcgct atcatggtgt acataattaa ggtggagcca gcagggatgc
     5641 gcttcatcaa tcacatctgt attttccagc ggtttaacgc cgcctctacc atccagcatc
     5701 caggcaaata ctgcatccgg gacattaacg tccgatccct taatcgcttc cacagtgcct
     5761 ccatcatcaa cgcattattt tgtagtctag ccttctggcc ctgttacgca acatctcatc
     5821 accccattac cctgaaatga ttaataaaat tctgtctaaa ttgaatacaa aaagcaaaat
     5881 gcttttccgt atacaaaccg tgtgaagtgt taaatagcgt ctatcattat cagaattatc
     5941 tgatcatatg acgtggcttt tttgcgatcg gatagcaaca aaaattgata aaaataatgg
     6001 gatctcaatg attacgcaca acttcaatac tctggactta ctcaccagtc ctgtctggat
     6061 cgtttcgccc tttgaggaac agttaattta tgccaacagc gcggcgcgac tgttgatgca
     6121 agacctcacg tttagtcagc tacgaaccgg tccctattcc gtctcctcac aaaaagaact
     6181 gccgaaatac ctctccgatc tgcaaaacca acacgatatt atcgaaatcc tcactgttca
     6241 gcgtaaagaa gaggaaacag cattaagctg tcggcttgtt ttgcgagagc tgacagaaac
     6301 agaaccggtg attattttcg aaggtatcga agcgccggca acgctgggtt taaaagccag
     6361 tcgctcggca aattatcagc gcaaaaaaca aggtttttat gcgcgctttt ttctgactaa
     6421 ctctgcacca atgttgttga ttgacccgtc acgagatgga caaatcgtcg atgctaacct
     6481 cgccgcgctc aatttctatg gttataacca tgaaacgatg tgccagaaac atacctggga
     6541 aataaatatg ctcgggcgtc gcgtcatgcc tatcatgcat gaaatctcgc atttacccgg
     6601 tggtcataaa cctctgaatt ttattcataa gctggcggat ggttcgactc gtcatgtgca
     6661 gacctatgcc ggaccgattg aaatttatgg cgacaagctc atgttatgta ttgtgcatga
     6721 tattactgag caaaaacggc tggaggagca gctggaacat gctgctcacc atgacgcgat
     6781 gaccggatta ctgaatcggc gacagtttta tcacattacg gaaccaggcc aaatgcagca
     6841 cctcgccatc gctcaggatt acagcttatt gctcatcgac acagatcgtt ttaaacacat
     6901 taacgatctc tatgggcatt ctaaaggtga tgaggtgtta tgcgccctcg cccgcactct
     6961 cgaaagttgc gctcgcaaag gcgatttggt gtttcgttgg ggaggcgaag agtttgtctt
     7021 attactacca agaaccccac tggataccgc gctttcgctg gctgaaacta tccgcgtaag
     7081 cgtggcaaaa gtgagtattt cgggcttacc acgctttacc gtcagcattg gtgtggcgca
     7141 tcacgaagga aatgaaagca tcgatgaact gtttaaacgc gttgatgatg ctttgtatcg
     7201 ggcgaaaaat gatggacgca accgcgtgct ggcggcataa gccacggatg cgtctcgaga
     7261 tcaacgactg cgcttagcgt ggcgctccca gttttcttgc tttgcctgtg ccgttttacg
     7321 tagtgcgacg taacacgccc cgctgccgcc atgatgcggt agcgcggtac aatatgcctg
     7381 gacatcatca aattcggtca accagcgcgc cacatagctg cggacaatat tggcatgcga
     7441 tttatcatcc cgccctttac catgaataat cagcacgtta cgcaaaccat ccgccatggc
     7501 ttgttgaatg aaactgaaca tcattttgcg gcactcttcc accggctggc gcaaaagatt
     7561 caggctcgcc tgttgcggat atttaccact gcgcagctta tccagcaccc catgttgcaa
     7621 cccttcccgc cgaaactcca gcggctgact tagcgggatg atgtcgagaa atccggtggt
     7681 gaggaaatta tcaagttgca gcgtgtcgat acgctgcggc gcacgttggt tacgcgttgg
     7741 atgccagtgg acatcggtag cacgtttcag cggctggaca tcttccatgg cgtcaagaaa
     7801 cagcgatttg tcgtcaaggt tcatgttaca tcctcccgca attaagagcg cgatatgata
     7861 accagaccgg gtcggtccaa caacgtatta cccaaaattc cagtaataag ttccaaatat
     7921 tgc
//
LOCUS       DBFIFI010000199         7886 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.199, whole genome shotgun sequence.
ACCESSION   DBFIFI010000199 DBFIFI010000000
VERSION     DBFIFI010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7886
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.199"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..1156
                     /gene="espL4"
                     /locus_tag="N7B39_003998"
     CDS             <1..1156
                     /gene="espL4"
                     /locus_tag="N7B39_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
                     /protein_id="HGU2423882.1"
                     /translation="NLLMAIGQNHQLKQLMIQLQKMPELHRTEMLTAYNSINLPGLYL
                     AINYGNADIVETIFNSLSEPGYEGLLSKKNLMHILEAKDKNGFSGLFLAISRKDKNVV
                     TSILNALPKLAATHHLDNEQVYKFLSAKNSTSSHVLYHVMANGDADMLKIVLDALSLL
                     IRTCHLTKEQVLDLLKAKDFYGCPGLYLAMQNGHSDIVKVILEALPSLAQEINISASD
                     IVDLLTAKSLARDTGLFMAMQRGHMNVINTIFNALPTLFNTFKFDKKNMKPLLLANNS
                     NEYPGLFSAIQHKQQNVVEMVYLALSDHARLFGFTAEDIMDFWQHKAPQKYSAFELAC
                     ELGHRVIAELIFNTLNKMAESFGFTDNPRYIAEKNYMEALLKKASPHTVR"
     gene            complement(1125..2861)
                     /gene="aceK"
                     /locus_tag="N7B39_003999"
     CDS             complement(1125..2861)
                     /gene="aceK"
                     /locus_tag="N7B39_003999"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HGU2423883.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAEFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLHWQNKSRDIHYIIRHLTETLGPENLSKSHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(3045..4349)
                     /gene="aceA"
                     /locus_tag="N7B39_004000"
     CDS             complement(3045..4349)
                     /gene="aceA"
                     /locus_tag="N7B39_004000"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HGU2423884.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAEAIHAKYPGKLLAYNCSPSFNWQKNLDDNTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(4379..5980)
                     /gene="aceB"
                     /locus_tag="N7B39_004001"
     CDS             complement(4379..5980)
                     /gene="aceB"
                     /locus_tag="N7B39_004001"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HGU2423885.1"
                     /translation="MTEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDADWKIRGIPADLEDRRVEITGSVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEEHNNQVLNKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(6249..7178)
                     /gene="metA"
                     /locus_tag="N7B39_004002"
     CDS             complement(6249..7178)
                     /gene="metA"
                     /locus_tag="N7B39_004002"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HGU2423886.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPEVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            7335..7778
                     /locus_tag="N7B39_004003"
     CDS             7335..7778
                     /locus_tag="N7B39_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HGU2423887.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVKHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLDLAYVGE"
BASE COUNT         1962 a         1964 c         1976 g         1984 t
ORIGIN      
        1 gaatttgcta atggcaattg gtcagaatca ccaacttaag caactgatga ttcaactcca
       61 gaaaatgcca gaactacata gaacagaaat gttgactgcc tataatagta ttaacctccc
      121 cggtttatat ttggctataa attatggtaa tgcggatatc gttgagacta tttttaattc
      181 attgtcagag ccaggatatg aaggattact ctcgaaaaaa aatctcatgc atattctgga
      241 ggcaaaagat aaaaatggtt tttctggatt atttttagcg atatcacgta aggataaaaa
      301 tgttgtaacc tcgattctga acgccttacc taaactggcc gcaacacatc atttagataa
      361 cgaacaagtg tataaattcc tgagtgccaa aaatagtacg tccagccatg ttttatacca
      421 tgttatggcg aatggtgatg ccgacatgct gaaaattgtt ttggacgcgt tatctttgtt
      481 aattcgcaca tgtcatttga ctaaagaaca ggtactcgat ctcctgaagg caaaggattt
      541 ttatggttgc ccaggactat acctggcgat gcaaaatgga catagcgata tcgtgaaagt
      601 tattcttgaa gcattgccca gcctggccca ggaaattaac atttcagctt ccgatattgt
      661 agatcttctg accgctaaaa gtcttgcgcg cgacacgggt ttgtttatgg ccatgcagcg
      721 cggacatatg aacgttatta atactatttt taacgcatta cccactctgt ttaatacgtt
      781 taaattcgat aaaaaaaata tgaagcccct cctcctggca aataattcta atgaataccc
      841 aggtttgttt tcagcgatac agcataaaca gcaaaacgtt gtagagatgg tttatcttgc
      901 tttatctgac catgcacgcc tgtttggatt taccgctgaa gatattatgg atttttggca
      961 acacaaagcg ccacaaaaat actctgcctt tgagttggct tgtgaattgg gtcaccgggt
     1021 tattgctgaa ttaatcttta atacattaaa taagatggct gaaagctttg gctttacgga
     1081 taaccctcga tacattgcgg agaaaaatta tatggaagct ttactcaaaa aagcatctcc
     1141 ccataccgta cgctaaatct ttgcctgcgc cgatacgcat aaacatcttc cacatgccct
     1201 tcacgtatgc ggttttgtag tgcgcgccag taatcagcgc ggaacaggtc ggcgtgcatc
     1261 tcttcaaaca gcggaccaat acgcgggtcg gcgcatagcc aatggcgaaa ctcttccggg
     1321 aaaacatcgc ccggcgagac gctgtaccac ggttcgctgg caagttcgtc ttccgggtag
     1381 cgcggcggcg ggatatcgcg gaaattcact tccgtcatgt agcaaatttc atcgtaatca
     1441 taaaaaacca cacgcccgtg acgggtgaca ccgaagtttt taaacagcat gtcgcccggg
     1501 aaaatgttag cagcggcaag ctggcgaatg gcattgccgt actcttcaat ggcgtcgcgc
     1561 agctgctgac cttccacttg ttccagccag atattgagcg gcaccatccg ccgctcaata
     1621 taaagatggc gaatcacaat ttgttcgccg agatcggtga ttttttccgc tgcttcctgg
     1681 agcagtaatt ccattaatgc cggggaaata tgccgcttct ccagcacaaa gttttcaaac
     1741 tcctgggtat ccgccattcg gcccacgcga tcgtgctctt tcaccagttg atagcaggca
     1801 cgaacgtgag cggcagacat ctctttctgc ggcgcgaact tgtctttgat gactttaaat
     1861 acacggtcaa agcccggcag cgtgaacacc aacatcacca tgccacgaat acccggcgct
     1921 tcaatgaact gctcattaca gccctgtaga taaacgagat attcgcggta gctttcggtt
     1981 ttggcgtgct tctggcagcc gatagccata tacaattcag cggtggtttt acctggcaga
     2041 atttcccgta accactcgac cagcgctgcg ggcagcggcg cataaaccat aaaataagaa
     2101 cgcgcaaagc caaaaacaat gctcgcttcg gcggtcgtcg tcagacaggt atcaataaac
     2161 agttcgccgt catccgtctg gtggatcggc aataaaaatg gcaatgtgcc ggaaggcgtt
     2221 attagtttgc ccaccagcca ggcggcttta ttacggtaaa acagttcgtt cgccacctgt
     2281 aaatgacttt tggagaggtt ttctggcccc aacgtttccg tcagatggcg aataatgtaa
     2341 tggatgtcac ggcttttatt ctgccagtgc agacgcaacg gcaggtcgct gataacgcgc
     2401 atcagcagag attcccagcc gtgatcgggg tgaaagtctt tcgccagcgg gcggggaatg
     2461 gtacgaaagc ggcgctctgg ctgggagcta aagataaaaa gccgctcggg agtaagcgag
     2521 cggtggtcaa ataaccgaca gtacacggag ttaaaaaagc tctccgcaat ctcgaagcgc
     2581 gggtaatccg gcaacagccg ggtgtaatgc tctttgacgc gcagtaaaaa ttccgcgtcc
     2641 gtgctttggc cgttggtaat gcagcgcagt tgctccacga ccagaccaac gtgatgatcg
     2701 taaagatgga tacggttttt catcgcctgc tggacagcat gccagtcggc ttgttcgaaa
     2761 cgctgctgcg ccccggaagt cacttcgagg aatcgaccat actgagcgtc gaagccttgc
     2821 aaaatggttt gagcaatcag taattccagg ccacgcggca tttagcgccc tcatcaggag
     2881 cagagaattg ccggatgcgg cgtaaacgcc ttatccagcc tacgttcggc aacggttgta
     2941 ggcatgataa gacgcgcaag cgtcgcatca ggcatcgtgc accgattgcc ggatgcggcg
     3001 tatatgcctt atccggccta cagtcagcaa cgtttgtagg ttgcttagaa ctgcgattct
     3061 tcggtggagc cagtcagcgc ggtaaccgac gacgtgccgc cctgaataat ggtcgtcact
     3121 ttatcgaagt aacctgtacc cacttcctgc tggtgagata cgaaggtgta gccatctttc
     3181 gcggcggcga attccggctg ctgcactttc tcaacatagt gcttcatacc ctcgccctgg
     3241 gcataggcgt ttgccagatc aaacatgttg aaccacatgc tgtggatacc tgccagggtg
     3301 atgaactgga acttgtagcc catatccgac agctgctgct ggaagctggc aatagtgttg
     3361 tcgtcgaggt ttttctgcca gttgaacgac ggcgagcagt tataagccag cagtttgccc
     3421 ggatatttcg cgtggatagc ttccgcaaag cgacgcgcca gttccagatc cggcgtggag
     3481 gtttcacacc ataccaggtc agcatatggc gcatacgcca ggccacggct gatcgcttgc
     3541 tcaatgcccg catgagtacg gaagaagcct tcactggtac gctcgccggt aataaattcg
     3601 ctgtcatacg ggtcgcaatc ggaggtgatc agatccgccg catcagcatc ggtacgtgca
     3661 accagcaggg ttggaacgcc cgtcacgtca gctgccagac gcgccgcgac cagtttctga
     3721 atagcttcct gagttggcac caaaactttg ccgcccatgt gaccgcattt cttcactgac
     3781 gccagctgat cttcgaagtg aactgccgct gcaccggctt caatcatcgc tttcatcagt
     3841 tcaaaggcat tcaggacacc gccaaaaccg gcttccgcgt cggcaacgat cggcaggaag
     3901 taatcgacat agcgcggatc gcccggctca atgcccgcgg accattggat ctgatccgca
     3961 cggcggaaag tgttgttgat ccgctccacc acagctggca ccgagtttgc cggatagagc
     4021 gactgatccg gatacatgct ggccgccagg ttagcgtccg ccgctacctg ccatcccgac
     4081 agatagaccg cttcaatacc cgctttcgcc tgttgcagcg cctgaccgcc agtcagtgca
     4141 ccgaggctgt tgatgtagcc ttttttcgac tcgccatgca gcagacgcca cattttcgcc
     4201 gcgcccagtt gcgccagcgt gcattcagga ttgactgaac cgcgtaattt caccacatct
     4261 tccgcgctgt atgggcgagt aattccttcc caacgcggtt gagtccactc tttctgtaat
     4321 tcttcaattt gttgtgtacg ggttttcatg tgcagatgct ccatagttat gtggtggttt
     4381 acgctaacag gcggtagcct ggcagggtca ggaaatcaat taactcatcg gaagtggtga
     4441 tctgttccat caagcgtgcg gcatcgtcaa aacgcccctg ggagaaacgt tcttcgccca
     4501 gttcgctggc aatgactttc atctcttcgc ccagcatctg gcggaacaag gctttagtta
     4561 ccggtttgcc attgctcaac gttttttgat gatggatcca ctgccagatt gaggtacggg
     4621 aaatttcagc cgtcgccgca tcttccatca ggccataaat cggcacgcag ccgttgccgg
     4681 agatccatgc ttcgatgtac tgcacagcca cgcgaatgtt ggcgcgcata ccttcttcgg
     4741 tgcgttcacc atcacaaggt gccagcagct gatcggcagt aatcggcgcg tcttgttcgc
     4801 gcatcacttc aagctgattt ttacgggagc cgagaatgtc gttgaatacc gccattgccg
     4861 tgtccgcaag gcctgggtga gcgatccatg tgccatcgtg accgttattg gcttccagcg
     4921 atttatccgc ttttactttg ttgagcacct ggttattgtg ctcttcatct ttgctcggaa
     4981 taaacgccgc catgccgccc atcgcaaaag caccgcgttt atggcaggtt ttaatcaaca
     5041 ggcgtgagta agcattcagg aatggtttat ccatcgttac tgcctgtctg tctggcagga
     5101 cgcgatcggg atagtttttc aacgttttga tatagctgaa gatgtaatcc caacgaccgc
     5161 agttcagacc aacaatatgg tcacgcagcg cgtgaaggat ttcatccatc tggaacacgg
     5221 cgggcagcgt ttcaatcagc aacgtcgcct tgatggtgcc gcgcggcaga ttaaagcgat
     5281 cttctgcata gctgaagact tcgctccacc aggccgcttc ctgccaggac tgggttttcg
     5341 gcagatagaa atagggacca ctgccctttg ccaacagtgc ctgatagttg tggaagaaat
     5401 agagcgcaaa atcaaacagg ctgccgggga ttgcctcacc acgccaggtg acatgttttt
     5461 ccggcaagtg cagaccgcgt acccgacaaa tcaaaaccgc tggattgggc ttgagctggt
     5521 aaattttgcc tgcttcattg gtgtaactga tggtgccgtt aaccgcatca cgcaggttaa
     5581 tttgcccgtc gatcactttg ttccagtctg gtgccagtga atcttcgaaa tcggccataa
     5641 agactttcac attggcgttg agcgcgttga tcaccatctt gcgctctacg gatccggtaa
     5701 tctctacgcg gcgatcttct aagtccgcag gaatcccgcg aattttccag tcagcatcac
     5761 gaatggaagc tgtttccgaa ataaaatcag gcaacgttcc gttatcaata tcttgctgct
     5821 gctgaatgcg cgctgccaga agtttattgc gttgtggcgt aaaatgcgtc accagctcag
     5881 tcagaaattc taccgcttcg gcagtaagaa tttgcttctc ctgctcgcca tacggcctta
     5941 tgaaagccag ttcatcggtt gttgttgcct gttcagtcat cgtgcagctc ctcgtcatgg
     6001 atccgaaaac ttccccactg tgaacgaagg atcgttgtgc gcttttcgtt cagcacaact
     6061 aagactactc atttaaaaat caaaatcaaa aacaatttcc attttaatta aaaatacttg
     6121 ataacttatt gataacaata tgaataaaat ttattcacct actgaggtgt gtttcggaaa
     6181 taaaaaaggc acccgaaggt gcctgaggta aggtgctgaa tcgcttaacg atcgactatc
     6241 acagaagatt aatccagcgt tggattcatg tgccgtagat cgtatggcgt gatctggtag
     6301 acgtaatagt tgagccagtt ggtaaacagc aaattaccgt gactacgcca gctcgctcgc
     6361 ggtttgtttt gcggatcatt gtgcgggaaa tagttatacg gtacttccgg gtctagtccg
     6421 gcttccacat cgcggaaata ttcctgcgcc agcgtttgcg catcatattc gggatggccc
     6481 gtcacaaagg caatgcgctt atctttactg gcaaacagat atgcatcccc ttcttccgtc
     6541 tctgccagaa tttccagatc ggtgtaatca cgaatcaacg ctgccggaaa gtcagcatag
     6601 cgcgaatgcg gtgccaagaa tgaatcatca aagccacgcg tcagaagcgc atgaggatgg
     6661 agaatatgat gctcgtaaac gccagagagt ttgtcggtgc gagtttgctt aggaatgccg
     6721 tagaggatat tgagcgctgc ctgtaccgcc cagcagacaa acagcgtcga ggtgacgtga
     6781 tctttcgacc actccagcac ctgtttgatc tgcggccagt aagcgacatc attaaactcc
     6841 accaggccca gcggcgcacc ggttacaatc agaccgtcaa agttctgttc ctgaatatct
     6901 tcaaagttac agtagaagtt gttcagatgc tctgcgggcg tgttacgcga ttcacgggaa
     6961 tcgatgcgca acagctgaat atcgacctgc aaaggtgagt ttgaaagcag gcgcagaaac
     7021 tgattttcag tttcaatctt cttcggcatc aggttaagga tcagaacctt aagtggacga
     7081 atttcctgac cagacgcacg agaagttgtc atcacaaaga cgttttcttc acgcaagaaa
     7141 ttgacggcgg gtagctcgtc cggcacacga atcggcataa cctgattacc tcactacata
     7201 cgcttatacg tttagacatc cagatagctg aagataacca gaaaatttgc caatgtcgag
     7261 cctgcatgtt gaaggtgaga aagtttcaag gaaaagacta cgttagaata taagaatgac
     7321 taaaaggaga gaaaatggtt attagtattc gccgctcacg gcatgaggaa ggggaggaac
     7381 tcgttgcgat ttggtgtcga tctgtcgatg ccacccacga ttttttatca gcagagtatc
     7441 gggccgagct ggaagagctg gttcgttcct ttctgccgga agcaccgttg tgggtcgcgg
     7501 ttaatgaacg ggatcagccg gttgggttta tgttgttaag tgggcagcat atggatgcgc
     7561 tgtttatcga tcctgatgtg cgcggctgcg gcgtgggccg gatgctggtg aagcatgcgc
     7621 tctcaatggc cccggaactg acaaccaatg ttaatgagca aaatgagcag gcggttgggt
     7681 tctataagaa ggtgggtttt aaggttacgg gacgctctga ggtggacgat ttggggaaac
     7741 cgtatccgtt gctggatctg gcgtatgtgg gggaataagc tgccccctat cgtgcctgcg
     7801 taagctggat aaaacgcgcc agcgtcgcat ccggcatttt tttgcaccaa cgcaaaaagg
     7861 ccatccgtca ggatggcctt tcgctt
//
LOCUS       DBFIFI010000200         7884 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.200, whole genome shotgun sequence.
ACCESSION   DBFIFI010000200 DBFIFI010000000
VERSION     DBFIFI010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7884
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.200"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            192..1064
                     /locus_tag="N7B39_004004"
     CDS             192..1064
                     /locus_tag="N7B39_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HGU2423888.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQEQNIREKTDAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELCTESVWGQAREQFTGAVDRIFDTVESVCVASVARTVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            1436..4285
                     /gene="cah"
                     /locus_tag="N7B39_004005"
     CDS             1436..4285
                     /gene="cah"
                     /locus_tag="N7B39_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding autotransporter Cah"
                     /protein_id="HGU2423889.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSVPALAADKVVQAGETVNDGTLTNHDNQIVFGTANGMTISTGLELGPDSEENTGGQ
                     WIQNGGIAGNTTVTTNGRQVVLEGGTASDTVIRDGGGQSLNGLAVNTTLNNRGEQWVH
                     EGGVATGTIINRDGYQSVKSGGLATGTIINTGAEGGPDSDNSYTGQKVQGTAESTTIN
                     KNGRQIILFSGLARDTLIYAGGDQSVHGRALNTTLNGGYQYVHRDGLALNTVINEGGW
                     QVVKAGGAAGNTTINQNGELRVHAGGEATAVTQNTGGALVTSTAATVIGTNRLGNFTV
                     ENGKADGVVLESGGRLDVLESHSAQNTLVDDGGTLAVSAGGKATSVTITSGGALIADS
                     GATVEGTNASGKFSIDGTSGQASGLLLENGGSFTVNAGGQAGNTTVGHRGTLTLAAGG
                     SLSGRTQLSKGASMVLNGDVVSTGDIVNAGEIRFDNQTTPNAALSRAVAKSNSPVTFH
                     KLTTTNLTGQGGTINMRVRLDGSNASDQLVINGGQATGKTWLAFTNVGNSNLGVATTG
                     QGIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYT
                     SMLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGS
                     YGFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLN
                     LVHTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQ
                     LQYTWQGLSLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMTFGEGTSSRDTLRDSA
                     KHSVSELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEAR
                     IRENITLGVQAGYAHSVSGSSAEGYNGQATLNMTF"
     gene            4396..6780
                     /locus_tag="N7B39_004006"
     CDS             4396..6780
                     /locus_tag="N7B39_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HGU2423890.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVDEPDVLSDSKNENGLL
                     FDKRKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLITLQEKLATPAGQQVAQSLQQTGDTRELL
                     DILADDVWLKNEYHGEDEIFTGLASLNDLVRLAAAMGTEFPFDEYAEVQKLPVIDVAF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEMMMRDQLQKASAVLAVMDYTQMNSKADE
                     EVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNGALPVDEAWVDDFIREAFGPMVEEDDWKDSTKVNKKAEKL
                     WNISLIDQLITEVIQSSHSRAAALAVDSAAAKLMQNAENVSEYLSLRHQGLQQSIQSL
                     QAHITSLLADIQEIEECQNQVTGDVRMAMEDINTKTGELLTKVCASLEEELNDYFRSG
                     KRKEQQMLEEENSAQPRERNAFAFFHDIFGTGNQHDRMRDFDPDSPEIKFSDRRAALE
                     LMTQIESTVTSLHREAEAQFRPELEKIVSGIETGFRGTALYATENIAGRINARLEDEG
                     FTVKISFPAVSQLQTRLAVKINLSALMEERTETVTRRRRQSGVWGTVCRWFGTSDLGW
                     ENYDEDVSRSVININKAREEVMSLTRAYFGELQASIEQDINQPVRQEIDAFFCAFREK
                     VEQLRNTLIQSSEDHKRDQQAQERLTGRLQALNERVPELITDSKALREELETML"
     gene            6777..7682
                     /locus_tag="N7B39_004007"
     CDS             6777..7682
                     /locus_tag="N7B39_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HGU2423891.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLTGEGAARQNAINASLAQGVEASLRWLTEMTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLADQVHHKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQSETGQVNQLLDILRHKALTQMAQE
                     SGGSATVRLNTLDWLGGQGREQADNEWHDAINWLGDWCSEEQHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGAA"
     gene            7679..>7884
                     /locus_tag="N7B39_004008"
     CDS             7679..>7884
                     /locus_tag="N7B39_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HGU2423892.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECGTQISMVSGTSIG
                     AFNGAIIAASPDLSEAAVRLEALW"
BASE COUNT         1953 a         1987 c         2284 g         1660 t
ORIGIN      
        1 gcatgattca cctttatatt ctctcaggga aaccaaatac cactactcaa ctattaagaa
       61 caaaattcgg gatgtatgat tatcagaaga tacctgcatc ctaaataatt tcagacagat
      121 attatcgttg tgaatccccc gccacctttc cggtgcgcgg ggttttgtct tttttcaccg
      181 ggaatacatg tatgaatccg tctgatgcca ttgaggcaat tgaaaaaccg ctctcctccc
      241 tgccttactc gctttcccgt cacatcctgg aacatctgcg caaactcacc agtcacgaac
      301 ccgtgattgg cattatgggt aaaagcgggg ccggtaaatc ctcactctgt aatgcactgt
      361 ttcaggggga ggtcaccccg gtcagtgatg ttcacgccgg cacccgggaa gtgcggcgct
      421 tccgtctgag tggccatggt cacagcatgg ttatcactga cctgcccggg gtgggcgaga
      481 gccgggacag ggatgcagag tatgaagccc tgtaccgtga cattctgcct gaactggact
      541 tggtactgtg gctgattaaa gccgatgacc gtgccctgtc tgtggatgag tatttctggc
      601 gacacatact gcaccgggga catcagcagg tgctgtttgt ggtgacgcag gccgacaaaa
      661 cagagccctg ccatgaatgg gatatggcag gtattcagcc ttctcccgca caggaacaga
      721 acattcgcga aaaaacagat gcggtattcc ggctgttccg gcccgtacat ccggttgttg
      781 ccgtatcggc ccgcaccggc tgggaactgg atacgctggt cagtgcgctc atgacagcgc
      841 ttcccgacca tgccgccagt cccctgatga cccgcctgca ggacgagctg tgcacggaat
      901 ctgtctgggg gcaggcccgt gaacagttta ccggtgcggt ggaccggata tttgacacgg
      961 tggagagcgt ctgtgttgcc tctgttgcac gcacagttct gcgtgccgtc cgtgacacgg
     1021 tggtctctgt tgcccgcgcg gtatggaact ggattttctt ctgaacagtc tgccagggga
     1081 aatgtctgat tacattgcgt taaccaactg aaaacatgat acaaacacct catgcccctc
     1141 ctgtttctct tgcgtgactg ctctactgtt aatagaataa aacgatcgat aaaacaggta
     1201 tcgatcgttt atatcgatcg tttatagtgg ttaatggcct gcgaaaaggc agctgaatct
     1261 cttcatcatg cagaacggaa ttgcacacaa cagactgata cttctctgtc tgatgaaggg
     1321 ggagagcgtt tacgcccggg acgtatcgct gtgcccgata actctgtttt gtacctgccg
     1381 gtatccactt ttgtgggtac cggctttttt attcaccctc tgtaaggaaa agctgatgaa
     1441 acgacatctg aacaccagct acaggctggt atggaatcac attacgggca ccctggtggt
     1501 ggcctccgaa ctggcccgct cacggggaaa acgcgccggt gtggcggttg cgctgtctct
     1561 tgctgctgtc acatcagtcc cggcactggc tgctgacaag gttgtacagg cgggagaaac
     1621 cgtgaacgat ggaacactga caaatcatga caaccagatt gtcttcggta cggccaacgg
     1681 aatgaccatc agtaccgggc tggaactggg gccggacagt gaagaaaaca ccggtgggca
     1741 atggatacag aatggcggga tagccggaaa caccactgtc accacaaatg gtcgtcaggt
     1801 cgtgctggag gggggaacag ccagtgatac ggttattcgt gacggcgggg gacagagcct
     1861 gaacggactg gcggtgaaca ccacactgaa taacagaggc gagcagtggg tgcatgaggg
     1921 cggggttgcc accggtacaa ttatcaaccg cgacggttac cagagcgtta aaagtggcgg
     1981 gctggcaaca ggaaccatca tcaacaccgg cgcagaaggc ggccctgatt ctgacaactc
     2041 gtatacgggt cagaaggtcc agggaacagc agaatccacc accatcaaca aaaatggacg
     2101 gcagattatc ttattttccg ggctagcccg tgacactctc atttacgcag gtggtgacca
     2161 gtcggtacac ggaagggccc tgaataccac actgaatggc ggttaccaat atgtgcacag
     2221 ggacggactt gcgctgaaca cggtaattaa cgaggggggc tggcaggttg ttaaggcagg
     2281 tggcgctgcc ggtaacacca ccataaatca gaacggtgaa ctgagggtac atgccggcgg
     2341 ggaagccact gcagtcaccc agaacacggg cggtgcactg gttaccagta ctgctgcaac
     2401 tgtcatcggc acaaaccgtc tggggaattt cacggtggaa aacggtaagg ctgacggtgt
     2461 tgttctggaa tccggcggtc gtctggatgt actggagagc cattcagcac agaataccct
     2521 agtggatgac ggcggtaccc tggcagtgtc tgccggcggt aaggcgacaa gtgtcaccat
     2581 aacatccggt ggtgccctga ttgcagacag tggtgccact gttgagggga ccaatgccag
     2641 cggtaagttc agtattgatg gcacatccgg tcaggccagc ggcctgctgc tggaaaatgg
     2701 cggcagcttt acggttaatg ccgggggaca ggctggcaac accactgtcg gacatcgtgg
     2761 aacactgacg ctggctgccg ggggaagtct gagtggcaga acacagctca gtaaaggcgc
     2821 cagtatggta ctgaatggtg atgtggtcag taccggcgat attgttaacg caggggagat
     2881 tcgctttgat aatcagacga caccgaatgc cgcgctgagc cgtgctgttg caaaaagtaa
     2941 ctccccggta acgttccata aactgaccac cacgaacctc accggccagg gcggcaccat
     3001 caatatgcgt gttcgccttg atggcagcaa tgcctctgac cagctggtga ttaatggtgg
     3061 tcaggcaacc ggcaaaacct ggcttgcgtt tacaaatgtc ggaaacagca acctcggggt
     3121 ggcaaccacc ggacagggta tccgggttgt ggatgcacag aatggcgcca ccacagaaga
     3181 aggtgcgttt gccctgagtc gcccgcttca ggccggcgcc tttaactaca ccctgaaccg
     3241 tgacagcgat gaagactggt acctgcgcag tgaaaatgct tatcgtgctg aagtccccct
     3301 gtatacatcc atgttgacac aggcaatgga ctatgaccgg attctggcag gctcccgcag
     3361 ccatcagacc ggtgtaaacg gtgaaaataa cagcgtccgt ctcagcattc agggcggtca
     3421 tctcggtcac gataacaacg gcggtattgc ccgtggagcc acgccggaaa gcagcggcag
     3481 ctatggcttc gtccgtctgg agggtgacct gctcagaaca gaggttgccg gtatgtctct
     3541 gacgacaggg gtgtatggtg ctgcaggcca ttcttccgtt gatgttaagg atgatgacgg
     3601 ttcccgcgcc ggcacggtcc gggatgatgc cggcagtctg ggcggatacc tgaatctggt
     3661 acacacatcc tccggcctgt gggctgacat tgtggcccag ggaacccgtc acagcatgaa
     3721 agcgtcatcg gacaataacg acttccgcgc ccggggctgg ggctggctgg gctcactgga
     3781 aaccggtctg cccttcagta tcactgacaa tctgatgctg gagccacaac tgcagtacac
     3841 ctggcaggga ctctccctgg atgacggcca ggataacgcc ggttatgtga agttcgggca
     3901 tggcagtgca caacatgtgc gtgccggttt ccgtctgggc agccacaacg atatgacctt
     3961 tggtgaaggc acctcatccc gtgacaccct gcgcgacagt gcaaaacaca gtgtgagtga
     4021 actgccggtg aactggtggg tacagccttc tgttatccgc accttcagct cccggggtga
     4081 catgagcatg gggacagccg cagccggcag taacatgacg ttctcaccgt cccggaatgg
     4141 cacgtcactg gacctgcagg ccggactgga agcccgtatc cgggaaaata tcaccctggg
     4201 cgttcaggcc ggttatgccc acagcgtcag cggcagcagc gctgaaggct ataacggtca
     4261 ggctacgctg aatatgactt tctgattata cggtatcccg gaaatacgat ttcaggaaaa
     4321 ttcatgcagt aatgggaaag gacgctcatt gctgttcacc gcagttctga attattgaat
     4381 ttataaaggt gaatgatgca cgaaaagaac atcgccctgc tttgtgatga agccgaccgg
     4441 cttttgcaac tgaatatcaa tctgctccgg caaatggttg atgagccgga tgtgttatct
     4501 gacagtaaga acgaaaacgg gctgcttttt gataaacgga aagcgctgaa aagaattgag
     4561 gagctggagg gcgaacaaat caaaaccgcc cgcagggaga tggtgctggc tgtggtcggc
     4621 acgatgaagg cgggcaaatc aaccaccatc aacgccattg tggggcagga aattctgccc
     4681 aaccgtaacc gccccatgac ctcggtaccg acattgatcc gccacgttcc cggaaaaacg
     4741 gaaccggtcc tccatctgga acatattcag cccgtccgca atttattaat cacactgcag
     4801 gaaaaactcg ccaccccggc agggcagcaa gtcgcacagt ccctgcagca aaccggggat
     4861 acccgcgaac tactggatat tctggcggat gatgtctggc ttaaaaatga ataccacggg
     4921 gaagacgaaa tctttaccgg actggcatcg ttaaatgacc tggttcgtct tgctgcggca
     4981 atggggactg aatttccttt tgacgaatac gcagaagtgc agaaactacc ggtgatcgac
     5041 gtggcgttca gccacctggt gggaatggat gcatgtcagg gaacactcac actgctggat
     5101 acccccggcc ctaatgaagc cggacaacca cagatggaaa tgatgatgcg ggatcaacta
     5161 cagaaagcct ctgcggtgct ggcggtgatg gattacaccc agatgaactc gaaagccgat
     5221 gaggaggttc gtaaagagct taatgccatt gctgacgtct cagccggtcg tctgtttgtg
     5281 ctggtcaata aattcgatga aaaagatcgc aatggtgatg gtgcggatgc cgtacgccag
     5341 aaggtgccag caatgctgaa cagcgatgtg ctgcccgcct cccgcgttta tcccggctca
     5401 tcacgtcagg cgtacctggc taaccgtgcg cttcatgagt tacgaaaaaa cggtgcgctt
     5461 ccggttgatg aagcctgggt cgatgatttt atcagggaag ctttcggacc tatggtggaa
     5521 gaggacgact ggaaagacag cactaaggta aataaaaagg cagaaaaact ctggaatata
     5581 tctctgatcg atcaactgat aacggaagtc atacagagct cccattccag agcggcggca
     5641 ctggcggttg actctgccgc cgcaaaactg atgcagaatg cagaaaatgt cagcgaatat
     5701 ctgtcattac gtcatcaggg gctgcagcaa agcattcagt cactacaggc gcatattacc
     5761 agcctgctgg cggatatcca ggagattgag gaatgtcaga atcaggtgac cggtgatgtc
     5821 agaatggcca tggaggacat caataccaaa accggggaat tactgacgaa ggtctgtgcc
     5881 tcactggagg aggagctgaa cgactatttc agaagcggta aacgcaaaga acagcaaatg
     5941 ctggaggaag aaaattcagc gcagccccgg gagagaaacg cattcgcttt ctttcacgat
     6001 attttcggaa ccggaaatca gcatgacagg atgcgggact ttgatccgga cagccccgaa
     6061 ataaaattca gtgatcgcag ggcagccctt gaactgatga cgcaaatcga atcgaccgtg
     6121 accagcctgc accgtgaggc tgaagcacag ttccggcctg agctggagaa aatcgtcagc
     6181 gggattgaaa caggttttcg tggcacggcc ctgtacgcca cagaaaacat tgccggtcgc
     6241 atcaatgccc gcctggagga tgagggcttt accgtaaaaa tcagttttcc ggcagtcagc
     6301 cagttacaga cccggctcgc ggtaaaaata aatctgagtg cgcttatgga ggaaagaacg
     6361 gagaccgtca cccgtcgccg tcggcagagt ggcgtatggg gaaccgtttg tcgatggttt
     6421 ggcaccagtg acctgggctg ggaaaactat gacgaggatg tgagtcgcag cgtgatcaat
     6481 atcaacaagg ccagagagga agttatgtca ctgacccggg catatttcgg ggagctgcag
     6541 gcatccattg agcaggatat taaccagccc gtccgccagg aaatcgatgc ctttttctgc
     6601 gcattcaggg agaaagttga acaactgcgt aacacgctga ttcagagctc tgaagatcat
     6661 aaacgcgatc agcaggcgca ggaacggctt accgggcgac ttcaggcatt aaatgaaagg
     6721 gttcctgagc tcattactga cagtaaggcg ctgagggaag agctggagac aatgctgtga
     6781 cttcaccatt tattcaacaa attgctgata accgggtatg tcaggtactc acctgtcttc
     6841 ctgaaaaatt tgtggttgat ttcgcaaacg gaatcgatgt tgcacaggag catatccgca
     6901 cggccggaga gcgcacgttc ttccggcgtt taaaagaggg gctgaccggc gaaggcgctg
     6961 cacggcagaa tgccatcaat gcgtcgcttg cacagggcgt tgaggcgtcc ctgcgctggc
     7021 tgacggagat gacaacgtca cttgccacca caaactacgc gattacccgg gtaaacgaca
     7081 gggtcagttc actggtcagt gatacagcca ggctggcgca ttattccgca gatacgcggg
     7141 agcagttact caccctggcc gatcaggttc accataaact gaatcatctt gaagaaaaac
     7201 tccaccgtgt tgaccaggtt cagcgggcgc agttacatct tgagcagata ttctcatggt
     7261 ggagcgccgg gcgatatgcg tcattttccc ctgccggacg ttgttatgtg gcacttgaag
     7321 agcttcgctg gggcgcgttt ggtgatgtga tacgtcagag cgaaacaggc caggttaacc
     7381 agctactgga tattctcaga cataaagcat taacgcagat ggcacaggag agtggcggta
     7441 gtgcaaccgt gcgtctgaac actctggact ggcttggtgg tcagggccgg gagcaggcag
     7501 ataacgagtg gcatgacgcg attaactggc tgggagactg gtgcagtgaa gagcagcatc
     7561 cggtgatctg gtcaaccacg caggctgcag aacacttacc ggttcgcatg cctcgcctct
     7621 gctctgcaga acgcctctct gaaagtatgg tcgatgaaat atttcagaag ggggctgcat
     7681 gagtacagaa atgaaaacgg ggctggtgct gtccggtggc ggggcggtgg gcgcttatca
     7741 ggcgggagtg gttaaggcac tggcagagtg tggtacacag atcagcatgg tttcagggac
     7801 cagcattggc gcattcaatg gtgccattat cgcggcctct cccgatctgt cagaagctgc
     7861 cgtacgcctg gaggcgctct ggga
//
LOCUS       DBFIFI010000201         7853 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.201, whole genome shotgun sequence.
ACCESSION   DBFIFI010000201 DBFIFI010000000
VERSION     DBFIFI010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7853
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.201"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..2175
                     /gene="pdeF"
                     /locus_tag="N7B39_004009"
     CDS             <1..2175
                     /gene="pdeF"
                     /locus_tag="N7B39_004009"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HGU2423893.1"
                     /translation="LILPIFAHINTFAHISSGEVFLFYLPLALMISMMMFFSWAALPG
                     IALGIFVRKYAELGFYETLSLTANFIIIIILCWGGYRVFTPRRNNVSHGDTRLISQRI
                     FWQIVFPATLFLILFQFAAFVGLLASRENLVGVMPFNLGTLINYQALLVGNLIGVPLC
                     YFIIRVVRNPFYLRSYYSQLKQQVDVKVTKKEFALWLLALGALLLLLCMPLNEKSTIF
                     STNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMISIHSYQNYIPIYPGYTTQLTITS
                     SSYLVFSFIVNYMAVLATRQRTVVRRIQRLAYVDPVVHLPNVRALNRALRDAPWSALC
                     YLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLEPGEDVYQLSGNDLALRLNTESHQ
                     ERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIVTN
                     APENMQRRGAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILLRM
                     KGENDELIGPDSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPTS
                     VCQARFPVEVSQLLDKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAIDD
                     FGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMRVVAEYV
                     ENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
     gene            complement(2214..3755)
                     /gene="ppx"
                     /locus_tag="N7B39_004010"
     CDS             complement(2214..3755)
                     /gene="ppx"
                     /locus_tag="N7B39_004010"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HGU2423894.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(3760..5826)
                     /gene="ppk1"
                     /locus_tag="N7B39_004011"
     CDS             complement(3760..5826)
                     /gene="ppk1"
                     /locus_tag="N7B39_004011"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HGU2423895.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(5997..6635)
                     /gene="purN"
                     /locus_tag="N7B39_004012"
     CDS             complement(5997..6635)
                     /gene="purN"
                     /locus_tag="N7B39_004012"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HGU2423896.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHPHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDVTARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(6635..7672)
                     /gene="purM"
                     /locus_tag="N7B39_004013"
     CDS             complement(6635..7672)
                     /gene="purM"
                     /locus_tag="N7B39_004013"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HGU2423897.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVNAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
BASE COUNT         1912 a         1981 c         1921 g         2039 t
ORIGIN      
        1 ttaatcttgc ccattttcgc ccacattaat actttcgcgc atatttcttc cggtgaggtt
       61 tttctctttt atctgcctct ggcactgatg atcagcatga tgatgttttt cagctgggcg
      121 gcattgccag ggatcgcctt agggattttt gtccgcaaat atgcagagct gggtttttac
      181 gaaacgctct cattaacggc taattttatt atcattatca ttctctgttg gggcggttac
      241 agggtcttta ctccccggcg taacaacgtt tcacatggtg atacccgttt aatttcccag
      301 cgtatattct ggcagattgt gtttcctgca acgctgtttc tgatactttt ccagtttgct
      361 gcatttgtag gattactggc gagcagagaa aatctggttg gcgtcatgcc ttttaatctc
      421 gggaccttaa tcaattatca ggccttactg gtgggcaatc tgattggtgt cccgctgtgc
      481 tacttcatca ttcgggtagt gcgaaatccg ttttatttac gtagttatta ttcgcaatta
      541 aaacagcagg ttgatgtcaa agtcaccaaa aaagagttcg cactctggct gctggcatta
      601 ggtgctttac tgttgctgtt atgcatgccg ttaaatgaaa aaagcacaat ttttagcacc
      661 aattatacct tgtcattatt gctgcccctg atgatgtggg gagcgatgcg ctatggttat
      721 aagctgattt cgctgctctg ggcggtcgtg ttgatgatca gcatccacag ctatcaaaat
      781 tacattccca tttatcctgg ctataccacg cagctgacca taacctcctc cagttatctg
      841 gtattctctt ttattgtcaa ttatatggct gtactggcaa cccgtcagag aacggtagtc
      901 agacgcattc agcgccttgc gtatgtggac ccggtggttc atctgccaaa tgttcgcgcc
      961 ctgaatcgcg cgttgcgtga tgccccctgg tctgcgcttt gttatttacg catccctggc
     1021 atggaaatgc tggttaaaaa ctatggcatc atgctgcgga ttcaatacaa gcaaaaactt
     1081 tctcactggc tgtcaccctt gctggaacca ggtgaagatg tttatcagct ttcgggtaac
     1141 gatctcgcgc tgcgactgaa tacagaatcg caccaggagc gcattaccgc actggatagc
     1201 catctcaagc aatttcgttt cttttgggat ggcatgccga tgcaaccgca gattggcgtc
     1261 agttactgct atgtgcgctc gccagtgaat catatctacc tgctgctggg agagctaaat
     1321 acggtcgccg aactttccat cgtgaccaac gccccggaaa atatgcagcg tcgcggggca
     1381 atgtatttgc aacgcgaatt gaaagataaa gtcgcgatga tgaatcgact acagcaggcg
     1441 ctggaacaca accatttttt cctgatggcc cagccgatta ccggtatgcg tggtgatgtt
     1501 taccatgaaa ttcttctgcg catgaaaggt gagaatgatg aactaatcgg ccccgatagc
     1561 ttcttgccgg tcgcgcacga atttggttta tcgtcgagta tcgacatgtg ggtcattgag
     1621 catacgctgc aatttatggc tgaaaacaga gcgaagatgc ccgctcaccg ttttgctatt
     1681 aatctgtctc caacctcggt atgtcaggct cgttttcctg ttgaagtcag tcagttgctg
     1741 gataaatatc agattgaagc gtggcaactt atttttgaag tcaccgaaag taatgctctg
     1801 accaatgtta agcaggcgca aatcaccttg cagcatcttc aggaattagg ctgccagatt
     1861 gcgattgatg atttcggcac cggctacgcc agctatgcgc ggcttaaaaa tgtgaatgcc
     1921 gatctgctta aaattgacgg cagttttatc cgcaatattg tgtcaaatag tctggattat
     1981 cagatagtgg catcgatttg ccacctggcg cgaatgaaga aaatgcgggt agtggcagag
     2041 tacgttgaaa acgaagagat ccgcgaggcg gtgctctctt tggggatcga ttatatgcag
     2101 ggttatctta ttggtaagcc gcaaccgtta attgatacgc tgaatgaaat cgaacccatt
     2161 cgcgaaagtg cctgaataat gcgggccgac atttctcgtc ggcccgcaaa gcattaagct
     2221 gcgatttcag gtgtactttc ttcttcaatt ttcaaccgcc agccagccac gccttcccag
     2281 tattcttgct ccttttccag atcaagcagt accagcgcat tctgactaaa ccagtcatgc
     2341 gggaaacgca gtgtccagtg actgtcatca gtaatcagcg tcaatgttgg cggtgtggtg
     2401 gttgcctgac gctgattgtt gagtaatacg ccaaggcgca atagctgaat cagtggcagg
     2461 aactgtttct tcttaaacaa ggtaaagcgc ggcagatcgt cgagtttaat cgctttacgg
     2521 tgatagcgca ccagtgtcgc catcatcagc tgctgttcct gattaaaacc cggcaagtcg
     2581 ctgttttgta gaatataagc ggagtgacga tgcaaaccgc tgtggttgat attcaacccg
     2641 acctcatgca gcatggcggc ccatcgcagt agcgcctcca gttgcggatg cgccagcttc
     2701 ggttgctgtt cccgccactg ttcgtacatt tgcatagtgg tatccagcac ccgtcgggcc
     2761 tgttcgctgt cgatgtgata ctggttggcg aggctgctgg cggtgcgact acgcacatcc
     2821 tgatgacgga aacgtccttc catttcatac agtacgcctt cgcgtaacgc cccgtcagaa
     2881 aggcgcagtt cacggatggc taaagcatca aacacaccgc ataaaatcgc tagtccagga
     2941 acgaagactg ttttccgctc ttcggaaaga cctggtaaac tcagcgatgc gaaattacgg
     3001 tgccgtaaaa cttcttttac cagtttttcc agacgttccg gggtaattat cccgtctttc
     3061 tcgcccattt ccattaacac ttcatgggcg gcttttatgg taccggaagc gcccattgcc
     3121 acgttccagc cctgaatacg aaattgccag gttaaagttt ccagtttttg tgctgccgcc
     3181 atgcgagcgc gctgaaaatt ctctttattg atgaccccgc cagggaaata aagctgggca
     3241 aagctgacac accccatccg gcggctttca acgagaatcg gttcgaaatt ttcaccaatc
     3301 accagttctg tagaaccgcc gccaatatca ataaccagtt tgcgaccttt ttccggttgg
     3361 gtatgttcca cgcccataaa aatcagacgg gcttcttcat taccggaaat aatttcaatc
     3421 gggtagggaa tgaccttttc cgcacgtttc agaaagtcag tggcgttcag cgcctgacgc
     3481 agcgtatggg taccaactat acagacgctg gcaggagaaa acccttgtag ccgttcggca
     3541 aacagcgaca gacagttcaa accgcgcgtc attgcctctt cactcaacat attatctggc
     3601 cccaggccgt ccgccagatg cacccgctgt ttcaggcggc caataatctg catggcacca
     3661 tctaccacac gggctatgac catgtgaaaa ctgtttgaac caagatcgac cgcagcaaac
     3721 tcctgcggac gaggggattt atcgtgtatt ggcatagggt tattcaggtt gttcgagtga
     3781 tttgatgtag tcataaatcg ccaactgcgc ccgtactttg cggcgattgc cgcggggaac
     3841 gtagcgatta ctgagttctt tatcgatata acgtgctttg accgtatcgc tgaacaatat
     3901 gtcgatgatg tccagcaccc gctgcttcag gcgcggatcg agcagcggcg tcgccacttc
     3961 aatacgataa tcaatattgc gcgtcatcca gtcggcggaa gaaaggtaga cctttttatc
     4021 gccgccattt tcaaaaatat aaacccggtc atgttcaagg taacggtcaa caatactgat
     4081 ggcacgaatg ttgtcgctaa tgccttccag attggggatc agcgaacaca ttccgcgaac
     4141 cagcagatta accggtacgc cggagctgga ggccgcatac agacgatcaa ccaggccttt
     4201 atcgacaagg ttatttagct tcagggtgat accactgggc agcccttgct gcgcgttggc
     4261 gatctcgcgg tccaccattt catacaacag gcggcgggag ttttgcggcg acaccattaa
     4321 ataatcaaat gtcaccggac ggtatgggtt ttcaataaag ttaaataccc gccgtacttc
     4381 gttggtgatg cgcgcatcgg cggtcagcaa cgaatagtca gtataaagac gcgcggtttt
     4441 ttcgttaaag ttcccggtcc cgatgtgtgc ataacgcacc acttcaccgt tttctttacg
     4501 tgaaatcaga aacagtttgg cgtgaatttt cagccccggc gcagagaaga taacgtgcac
     4561 gcctgcttcg gtcaggcgct tcgcccagtg aatgttggct tcttcgtcaa aacgtgcctg
     4621 tagctcaacc accacggtga ctttcttacc gttgtgtgcg gcgtggatca tcgagtcgat
     4681 gatgcgtgaa tcttttgcca cacggtaaat gttgatttta atcgccagca cgctcggatc
     4741 gaacgaggcc tgacgcagca gttccagcac atgctcaaag gtgtgataag gataatagag
     4801 caacacatcg cgttcgcgaa tggcatcaaa accattgcgg aactgggctt tatcaaacca
     4861 aatatggcgt aaacgcggca gtggtttgtt caccagattg gctttgccga cattggggaa
     4921 attaataaag tctttaaaat tatgataacg accgccgggg acgatggagt cgtagcggga
     4981 aatagtcagt ttttcgcgta acacttcaac cagcgcattg ggcatatcgc gctgataaac
     5041 aaaacgcacc ggctcagcag ttaaacgctg cttgagactg gaagacatca actccatcag
     5101 gctggcttcc atctcatgca ctaaatcgta ttcggcatcg cgggtcatct tcattgaata
     5161 ggcattcagc gcgtcataat caaagaagcc tttgaaaata tcatcaaggc agtaacgcag
     5221 aatgttatcc agaagaatca ttggcttgcg tcgacgcggc gcttctggcg gtaaattcac
     5281 aaagcgcggc actttatctg atgggatctc cagaagcgcg taacggatgg tatcgccacg
     5341 gataatttcc accgccagat aggtgtaatc atctttcagg aactgcacta agtcagtgtc
     5401 aggattgatt aaaatcggcg taatgtgctg acgcagatac tgcttaaaat aatgacgcag
     5461 ccagttttgt tgattgacgg agagctggcg ttcattaatc aggaagatct ggttgcgcgc
     5521 catctccagc aatagctcgt tgtagaggcc gtcgaattcc tgatcggctt tcagcacccg
     5581 ggactgaatt ttgcccagta aatggcggga atgagagttg gagccttgtt cttcgctaat
     5641 aatgatgcgt cgcttcagtt cagcgaagcg gactttatag aactcatcaa ggttattgga
     5701 atagatcccc aggaaacgca tcctttcaat cagcgggtta gatttgtccg ccgcttcctg
     5761 aagcacgcgt tcattgaacg ataaccaact gagctctttt tcgatgtata gcttttcctg
     5821 acccattacc acttttactc cgttttattc acgggacact gcctggatat tatggcgagc
     5881 aatattggca gatgtccaac tatgccagaa aagggggaaa aaaactgctg gagcttgcga
     5941 tttacaggtg aattgaaaaa cgccagcggc agagctggcg ctttaactac ggggcattac
     6001 tcgtcggcag cgtagccctg cggcggcaga cgttgaccat ccagccacgc ggcgttttcg
     6061 tgcattttca gacgaccatc ggcaaaccag ctaatcacca gtggataaat ggcgtgttcc
     6121 tgggtttgca cgcgggcggt gacgtcatcc tccgtatcac cagcaaatac cgggactttc
     6181 gcctgtaaaa taaccgggcc accgtccagt tcatcggtga cgaaatgcac cgatgtaccg
     6241 tgctcttcat cgccattttc cagcgcctga cgatggggat gtaatccggg atatttcggc
     6301 agcagagaag ggtgaatgtt cagcaaacgc ccggcatagt gggagacaaa tgccgggctg
     6361 agaatgcgca taaaaccagc cagcacgacc acatcgggtg cgtacatgtc gatttcatga
     6421 atcaactccc ggtcataggc ttcacgactg tcaaacgcgc tggcgatgag cgtatgcgtt
     6481 gcaatacccg cctggcgggc gcgttcaagg ccgaacgcgt cggccttatt gctgaaaact
     6541 gcccgtacgg tgcctttaat tttgttggtt ttacaggcgt caataattgc ctgcaaatta
     6601 cttccgttgc cggaaataag caccacaata ttcattattc gataaccacg cgttgttcgg
     6661 aatcagaggc tttgatgata ccgattttcc acgcgttttc accgttggca ttgagcaggg
     6721 cgagggcttt gtccacttcc ggagcaggca gggcaataat catcccgacg ccgcagttga
     6781 aggtgcgata catttcatgg cgctcaacgt taccagccgt ttgcagccag ttgaacactt
     6841 ccggccactg ccaggaggat tcatcaatca ctgcctgggt attatctggc aatacgcgcg
     6901 gaatgttttc ccaaaagccg ccgccggtca ggtgcgcaat ggcattaaca tcgacctttt
     6961 caatcaactc cagcactgac ttcacgtaaa tgcgggtcgg tgccagcaga tgatcggcta
     7021 atggcttacc atcaagttcg gtggtttgcg gatcacaacc gctgacttca agaattttgc
     7081 gcaccagcga atagccgttc gagtgcggac cgctggaacc gagtgcaatc agcacatcgc
     7141 cgtcgctgac tttagagccg tcgatgattt ctgatttttc taccacgccc acgcagaaac
     7201 ccgcgacatc gtaatcctca ccgtgataca tccccggcat ttctgccgtt tcgccaccca
     7261 ccagtgaaca gcccgattgc agacaacctt ccgcaatgcc gctgatcacc gctgaagcgg
     7321 tatcaacatc cagttttccg gttgcgtaat agtcgaggaa aaacagcggt tctgcacctt
     7381 gcaccaccag gtcattaacg cacatggcga ccagatcaat accaatggtg tcgtgacgtt
     7441 ttaagtccat tgccagacgc agcttggtac ctacgccgtc agtgccggaa accagcacgg
     7501 gttcacgata tttttgcggc aatgcacaca gcgcaccgaa gccgcccaga ccgcccatca
     7561 cttccggacg acgcgttttc ttcactacgc ctttgattct tccaaccaga gcattacccg
     7621 cgtcaatatc aacaccggca tctttgtagc taagagaggt tttatcggtc actgcttggg
     7681 tccccacgcg ttacttgcgg tagaaaaata aaattcggcg caattctaac agggaaagca
     7741 aacgtttgcg agactgcttt acacaacctt tttgcacgtc ttttccccag gcgcgcggcg
     7801 aaagaagact tgtgccaggg caaaggttag ttttcggatg gaataatctt ctt
//
LOCUS       DBFIFI010000202         7625 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.202, whole genome shotgun sequence.
ACCESSION   DBFIFI010000202 DBFIFI010000000
VERSION     DBFIFI010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7625
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.202"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            74..523
                     /gene="alaE"
                     /locus_tag="N7B39_004014"
     CDS             74..523
                     /gene="alaE"
                     /locus_tag="N7B39_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HGU2423898.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(560..904)
                     /locus_tag="N7B39_004015"
     CDS             complement(560..904)
                     /locus_tag="N7B39_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HGU2423899.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            1056..1385
                     /locus_tag="N7B39_004016"
     CDS             1056..1385
                     /locus_tag="N7B39_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HGU2423900.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            complement(1535..2869)
                     /locus_tag="N7B39_004017"
     CDS             complement(1535..2869)
                     /locus_tag="N7B39_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HGU2423901.1"
                     /translation="MPRYQDIARQLKTVIEQGKLKPGARLPSSRTWSQELGVSRSTVE
                     NAYAELVAQGWLVRRGQAGTFVSEQIHPQQSAVDVVAFAGESQQPLPFQMGLPALDLF
                     PRELWARVMGRRLRTQTRFDLALGDVCGEAALREAIVDYLRVSRGIDCQPEQVFITHG
                     YAASMALILHALAQPGDGMWMEDPGFPLIRPIVTRHGVEILPVPVDDNGLDVTSGIQN
                     YPDARFALITPAHQSPLGVALSLARRYQILEWAERSQAWIIEDDYDSEFRYHGKPLPA
                     LKSLDAPQRVIYAGTFSKALFPALRCAWLVVPVEQISQFRQQASLEPCAVPVLWQNTL
                     ADFIREGHFWRHLKKMRQHYAQRRQWIEQALTQQGFQVVPQKGGIQMVIKLAGNDVTF
                     VHKANAAGLAVQALSDWRIRSSEDGGLLLSFTNIVSESMARQVAQQLRSALN"
     gene            2957..3388
                     /locus_tag="N7B39_004018"
     CDS             2957..3388
                     /locus_tag="N7B39_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HGU2423902.1"
                     /translation="MTTLRQPYYELSPAVYNALVQAKTALENSTLDTTLMELIYLRVS
                     QINGCAFCLEMHSKALRKSGVPQHKLDALAGWRVSHHFDERERAALAWAESVTDIART
                     HAEDEVYQPLLEHFSAAEISDLTFAIGLMNCFNRLAVSMRM"
     gene            3597..3842
                     /gene="nrdH"
                     /locus_tag="N7B39_004019"
     CDS             3597..3842
                     /gene="nrdH"
                     /locus_tag="N7B39_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HGU2423903.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            3839..4249
                     /gene="nrdI"
                     /locus_tag="N7B39_004020"
     CDS             3839..4249
                     /gene="nrdI"
                     /locus_tag="N7B39_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HGU2423904.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            4222..6366
                     /gene="nrdE"
                     /locus_tag="N7B39_004021"
     CDS             4222..6366
                     /gene="nrdE"
                     /locus_tag="N7B39_004021"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HGU2423905.1"
                     /translation="MATTTAECLTQKTMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADVAISQHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPVAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTGKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            6376..7335
                     /gene="nrdF"
                     /locus_tag="N7B39_004022"
     CDS             6376..7335
                     /gene="nrdF"
                     /locus_tag="N7B39_004022"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HGU2423906.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQHEEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEYEDWNF"
BASE COUNT         1879 a         1963 c         1901 g         1882 t
ORIGIN      
        1 tgcagatggc aatgagatcc actgctttca tctccattaa catcccatta cgcttttatt
       61 aaggagcatt agcatgttct caccgcagtc acgcttgcgt catgcagttg cagatacgtt
      121 cgcgatggtt gtttactgtt ctgtcgtgaa catgtgtatt gaagttttcc tctccggaat
      181 gagcttcgaa cagtcttttt attccagatt ggtagcgatt ccggtgaaca tcttaattgc
      241 atggccatac ggtatgtacc gcgatctgtt tatgcgcgcg gcacgcaaag ttagcccgtc
      301 gggctggata aaaaatctgg cggatatcct ggcttatgtg acgttccagt caccggtgta
      361 tgtggcgatc ttgttagtgg tgggcgcaga ctggcatcag attatggcgg cggtcagttc
      421 aaacatcgtt gtttcgatgt tgatgggggc ggtttatggc tacttcctcg attattgccg
      481 ccgactgttt aaagtcagcc gttaccagca ggtaaaagcc tgactcttct tttcgcgact
      541 ggcatcgcca gtcgcgtctt taactggctt cgccaaataa accattcaaa taacgttcaa
      601 gcgcaacacg cgaactaaag ccatgcggga taccgtaatg ctcgtgtcgc tcgcctgcta
      661 aatagagcgg aaattgctgg taatgatcgc aagttttaat atcggaagcg ggttcagcca
      721 gtgtcgaact gcgttctttt aatgttggat gaattaccag agcggtacgt cccattcgcg
      781 cttcgcgatt aacatagaca taattttccc cacggcggta accatacgct ttctgggtta
      841 ccacatcgac agtaaaaccg actttttcaa gtacgcgcgc cacctcgtct ggtcgtaaat
      901 acatatttga tcctcgttat tatcaaccgc gggcctacct tacctgattg cgcattcaca
      961 acgctttcag aaaagtccat aaagccgcga tgaacctcag ttaagaaaat tatggtctac
     1021 actgaaaatt acatcgaatt ctaatggggg atcatatgtt taaccgaccg aaccgcaacg
     1081 acgttgatga tggcgtgcag gatatccaga atgatgtcaa tcaattagct gacagcctcg
     1141 aatctgtatt gaagtcctgg ggcagcgacg ccaaagggga agctgaagcc gcacgcagta
     1201 aagctcaggc attgctgaaa gaaaccaggg cgcgaatgca tggtcgtact cgcgtccagc
     1261 aagccgcgcg cgatgccgta ggctgcgcgg attcttttgt tcgtgaaaga ccctggtgta
     1321 gtgtgggaac tgctgctgcg gtaggtattt ttatcggtgc gttgttgagc atgcgtaaat
     1381 cgtagtgaga cgatgacaag cagtagtctg acttctgaaa agtactcagt gcgtcaacgc
     1441 cttgtccgcc tacaggcctg aaaaacatag cgcatcaggg gattttgccc tggtcaccag
     1501 tcttatagga ccccgaagcc tggcttcggg gttattaatt taaagcactg cgcaattgct
     1561 gtgctacctg tcgcgccata ctttcgctaa cgatattcgt aaacgagagc aataatcctc
     1621 cgtcctcgct tgagcggata cgccagtcgc taagtgcctg tacggcaaga ccagcagcat
     1681 tggctttatg cacgaatgtc acgtcattac ctgctagttt gatcaccatc tggataccgc
     1741 ctttttgcgg cacaacctga aatccctgct gcgtaagtgc ctgctcaatc cactgccgtc
     1801 gctgggcata atgctggcgc attttcttca gatgccgcca gaaatgcccc tcacggatga
     1861 aatctgccag tgtgttctgc cacagtacgg gtacagcaca tggttccagt gacgcctgct
     1921 gccggaattg cgaaatctgc tccaccggca ccaccagcca cgcacagcgc aatgcaggaa
     1981 atagcgcttt gctgaatgtt ccggcataaa tcacccgctg cggcgcgtcg agacttttta
     2041 gtgccggtaa cggcttaccg tgatagcgaa actcactgtc gtaatcatct tcaataatcc
     2101 atgcctgact acgctctgcc cattccagta tctgatacct acgcgctaaa gagagtgcca
     2161 cacccagcgg gctttggtgt gccggagtaa tcagggcaaa acgcgcatca ggataatttt
     2221 gtattccgct tgtgacatcc agtccgttgt catcaaccgg cacaggtaaa atttccacac
     2281 cgtggcgagt gacaatcggg cgaatcagcg gaaagccggg atcttccatc cacatcccgt
     2341 ctcccggttg cgccagagcg tgcagaatta aagccattga ggccgcataa ccgtgagtga
     2401 taaagacctg ctctggctga caatcaatcc cacgtgaaac gcgtaaataa tcaacaatag
     2461 cctcgcgcaa ggccgcctcg ccgcagacat cgcctaatgc caaatcaaag cgcgtctggg
     2521 tacgcagacg gcggcccatc acccgcgccc acaattcacg cggaaaaaga tcaagtgcag
     2581 gtagccccat ctgaaaaggc agcggctgct gactttcacc agcaaaagcc acgacatcca
     2641 cagcagactg ttgcggatgt atctgctcac tgacaaaggt acccgcctgt ccccgcctga
     2701 ccagccatcc ctgcgccacc agctcagcat aagcattttc gacagtggat cgagagacac
     2761 ccagctcctg cgaccaggta cggctcgaag gtagccttgc gccaggtttc agttttcctt
     2821 gctcgataac cgtttttaac tgacgggcga tatcctgata acgtggcata tggtctgctt
     2881 ttttaggtaa tttatggctc tctttagcag tccattatag tgctacattg cgggcgttaa
     2941 taagaggaga aactgtatga ctacattacg tcagccttac tacgaactta gcccggcagt
     3001 gtataacgca ctggtgcagg ccaaaacggc actggaaaat agcacgctgg ataccacgtt
     3061 gatggagtta atttatttgc gcgtctcgca aatcaacggc tgcgcatttt gtctggagat
     3121 gcacagcaaa gcattgcgca aatccggtgt gccacaacac aaactggacg ccctggcagg
     3181 ctggcgcgta agccatcatt ttgatgaacg cgagcgagcg gcgctggcat gggcggaatc
     3241 tgtgaccgat attgccagaa cccatgcgga agacgaggtt tatcagcctt tgcttgagca
     3301 tttcagcgca gcagaaatca gcgacttaac gtttgccatc gggctgatga attgttttaa
     3361 ccgtctggcc gtttccatgc ggatgtaatt tgctgttgcc gggtatgcgc ccggcaaaaa
     3421 aacacaaaat cccctacccc gttgagccat tcaccggtgg cgtaatcaat ctaaaattac
     3481 catatcttgt gtggttgaat cttttttcaa ctacatctag tatctctgta tcaacagaga
     3541 gacaacccga cgcgtatcat cgcgccgtat cttcatttta aacggaaata cgaatcatgc
     3601 gcattactat ttacactcgt aacgattgcg ttcagtgcca cgccaccaaa cgggcgatgg
     3661 aaaaccgggg ctttgatttt gaaatgatta atgtcgatcg cgttcctgaa gcggcagaag
     3721 cgttgcgtgc tcagggcttt cgtcagttgc cggtagtgat tgctggcgat cttagctggt
     3781 ctggtttccg tccggacatg attaaccgtc tgcatccagc gccacacgcg gccagtgcat
     3841 gagccagctc gtctacttct ccagcagctc cgaaaacacg cagcgtttta tcgaacgttt
     3901 aggtctgccc gcggtgcgca tcccgctcaa tgagcgggaa cggattcagg tagacgagcc
     3961 ttacatcctg atcgtgccct cttacggcgg cggcggtacg gctggcgcgg tgccacgaca
     4021 ggtaattcgc tttttaaacg acgagcacaa ccgggcgttg cttcgcggcg ttattgcttc
     4081 gggtaatcgc aactttggtg aggcgtatgg ccgcgccgga gatgtgattg cccggaaatg
     4141 cggcgtgccg tggctgtacc gttttgaact catgggtacg caaagcgata tcgaaaacgt
     4201 tcgtaaagga gtaaccgaat tttggcaacg acaaccgcag aatgcctgac gcagaaaacg
     4261 atggattacc acgcgctgaa tgcgatgctt aacctctacg atagcgcagg tcgcattcag
     4321 ttcgataaag accgccaggc cgttgacgcc tttatggcga cgcatgtgcg tccgaacagt
     4381 gtgaccttca gtagccagca gcagcgcctg aactggctgg tcaacgaagg ttactatgat
     4441 gaaagcgttc ttaatcgcta ctctcgcgac tttgtcatta cgctgtttgc ccacgcacac
     4501 accagcggtt ttcgtttcca gacattcctc ggggcatgga agttttacac cagctatacg
     4561 ttgaagacat tcgacggtaa acgttatctg gaagattttg ccgatcgagt cacgatggtg
     4621 gcgctgacgc tggcacaagg cgatgagacg ctggcgttgc aactgacaga tgaaatgctg
     4681 tcaggacgct ttcagccagc cacgccaaca ttcctcaact gcggtaagca gcagcgcggc
     4741 gaactggttt cctgtttttt gctgcgtatt gaagacaata tggagtcgat tggtcgggcg
     4801 gtaaattccg cactgcaact gtcgaaacgc ggcggcggcg tagcattttt gctgtcgaat
     4861 ctgcgggaag cgggcgcgcc aattaaacgt attgaaaatc aatcttctgg cgtaattccg
     4921 gtgatgaaaa tgctggaaga cgcattttcc tatgccaacc aactcggcgc tcgtcagggg
     4981 gctggtgcag tctatttaca tgctcatcat cccgatattc tgcgttttct cgacacgaaa
     5041 cgggaaaatg ccgacgaaaa aatccgcatt aaaacactgt cgcttggcgt ggtgatcccg
     5101 gatatcactt tccatctggc aaaagagaat gcgcagatgg cgctgttttc gccttatgac
     5161 gtagagcgag tttatggcaa gccgtttgcc gatgtggcca tcagccaaca ctatgacgaa
     5221 ctggttgccg atgaacgcat tcgcaaaaaa tacctcaacg cccgtgattt cttccagcga
     5281 ctggcagaaa tccagtttga gtccggctat ccctacatca tgtatgaaga cacggtaaac
     5341 cgtgctaacc ctgtcgccgg gcgcataaat atgagtaatc tctgctcaga aattttgcag
     5401 gttaacagcg cctcagagta tgacgagaat ctcgactatg cccgcacagg ccatgatatc
     5461 tcctgcaatt taggttcgtt gaatattgcg cacaccatgg attcccccga ttttgcccgc
     5521 acggtagaga ctgccgtgcg cggtttaacg gcagtatcag atatgagtca tatccgcagc
     5581 gtgccgtcca tcgaagccgg aaatgccgcc tcgcacgcca tcggactggg gcagatgaat
     5641 ttacacggct atctggcgcg agaaggcatc gcttatggtt cgccggaagc actggatttc
     5701 accaatctct atttctatgc catcacctgg cacgcactgc gtacctcgat gttgctggca
     5761 cgcgaacgcg gtgaaacctt cgccgggttc aaacagtcac gctatgccag tggtgaatat
     5821 tttagccaat atctgcaagg gaactggcag ccgaaaacgg ggaaagttgg cgaactgttt
     5881 gcccgtagcg gtattacgtt acctacccgt gagatgtggg cgcaactgcg cgacgacgtg
     5941 atgcgctacg gcatttacaa ccagaatctt caggcggtgc cgccaacagg ttctatctct
     6001 tatatcaacc atgctacgtc gagtattcat ccgattgtgg cgaaagtaga gatacgcaaa
     6061 gagggcaaaa caggacgcgt ttactaccct gccccgttta tgactaacga gaatctggcg
     6121 ctgtatcagg acgcttacga aattggcgca gaaaagatca tcgacaccta cgcggaagcg
     6181 actcgccatg tcgatcaggg gctgtcgctg acgctgtttt tccccgatac cgccaccact
     6241 cgcgatatca acaaagcgca gatttacgcc tggcgcaaag gtatcaaaac gctctattac
     6301 atccggctgc gtcagatggc gctggaaggc actgaaattg aaggctgcgt ctcctgtgca
     6361 ctttaaggaa tatctatgaa actctcacgt atcagcgcca tcaactggaa caagatatct
     6421 gacgataaag atctggaggt gtggaatcgc ctgaccagca atttctggct accagaaaag
     6481 gtgccgctgt cgaacgatat tcctgcctgg cagacattaa ctgtcgtaga acaacaactg
     6541 acgatgcgcg tttttactgg cctgacgctg ctcgacacgc tgcaaaatgt tatcggcgcg
     6601 ccttcgctga tgcccgatgc actcacgcct catgaagaag cggtattatc gaatatcagc
     6661 tttatggaag cggttcatgc ccgctcttac agttcgattt tctcgacgct gtgccagacc
     6721 aaagatgtcg atgccgccta cgcctggagt gaagaaaacg cgccgttgca gcgaaaagca
     6781 cagattattc agcaacatta ccgcggtgat gatccgctga aaaagaaaat cgccagtgtg
     6841 tttcttgaat cttttttgtt ctattccggt ttctggctgc cgatgtattt ttccagccgc
     6901 ggaaagctga ccaataccgc ggacctgatc cgtctgatta tccgcgatga agcagtccac
     6961 ggttactaca taggctataa atatcagaaa aacatggaaa agatatctct gggacaacat
     7021 gaagagttga agagtttcgc cttcgatttg ttgctggaac tctacgacaa cgagttgcaa
     7081 tacaccgatg agctgtacgc cgaaaccccg tgggctgacg atgtgaaagc gtttctctgt
     7141 tacaacgcca ataaggcttt gatgaatctg ggctacgaac cgttatttcc cgcagaaatg
     7201 gcggaagtga atccggcaat cctcgccgcg ctttcgccga atgccgatga aaatcacgat
     7261 ttcttttccg gttcaggctc ctcttatgtg atggggaaag cggttgaaac agaatatgaa
     7321 gactggaatt tctgagggtg ttattttcaa aaatatcact acccgcgaca gggaaataat
     7381 tcccgccaaa tagcttttta tcacgcaaat attttgtggt gatctacact gatactctgt
     7441 tgcattattc gcctgaaacc acaatattca ggcgtttttt cgctatcttt gacaaaaaat
     7501 atcaactttt ctcgatttgc tctcagccct tatatcacgg gaaattccgg cgatttgctc
     7561 gcatcaatat tcatgccaca tttgccatca ggggttgcct cagattctca gtatgttagg
     7621 gtaga
//
LOCUS       DBFIFI010000203         7538 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.203, whole genome shotgun sequence.
ACCESSION   DBFIFI010000203 DBFIFI010000000
VERSION     DBFIFI010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7538
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.203"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..290)
                     /locus_tag="N7B39_004023"
     CDS             complement(<1..290)
                     /locus_tag="N7B39_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2423907.1"
                     /translation="MNRINILVICMVLFFMTGNACATEWISSEDLITSDFHLMTADER
                     NVVKAATDDSMEAAYMLKDNIRWYYHNGDLSLPANFSNKNKLVVNGNLTISG"
     gene            complement(434..718)
                     /gene="ppnP"
                     /locus_tag="N7B39_004024"
     CDS             complement(434..718)
                     /gene="ppnP"
                     /locus_tag="N7B39_004024"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HGU2423908.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(790..1467)
                     /locus_tag="N7B39_004025"
     CDS             complement(790..1467)
                     /locus_tag="N7B39_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HGU2423909.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNHLAHVSRRKVERDLQGVVEVLDNQGYDVIILMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEELLTVQAQKWQILQKPPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFNQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(1725..1916)
                     /gene="yaiA"
                     /locus_tag="N7B39_004026"
     CDS             complement(1725..1916)
                     /gene="yaiA"
                     /locus_tag="N7B39_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HGU2423910.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(1966..2490)
                     /gene="aroL"
                     /locus_tag="N7B39_004027"
     CDS             complement(1966..2490)
                     /gene="aroL"
                     /locus_tag="N7B39_004027"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HGU2423911.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(2673..3131)
                     /locus_tag="N7B39_004028"
     CDS             complement(2673..3131)
                     /locus_tag="N7B39_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HGU2423912.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            3251..4060
                     /gene="proC"
                     /locus_tag="N7B39_004029"
     CDS             3251..4060
                     /gene="proC"
                     /locus_tag="N7B39_004029"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308464.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HGU2423913.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDKFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(4077..5192)
                     /gene="adrA"
                     /locus_tag="N7B39_004030"
                     /gene_synonym="dgcC"
     CDS             complement(4077..5192)
                     /gene="adrA"
                     /locus_tag="N7B39_004030"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HGU2423914.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVIYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVHEGLNTLRLPNTPQVNLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(5294..5614)
                     /gene="psiF"
                     /locus_tag="N7B39_004031"
     CDS             complement(5294..5614)
                     /gene="psiF"
                     /locus_tag="N7B39_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HGU2423915.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(5733..7148)
                     /gene="phoA"
                     /locus_tag="N7B39_004032"
     CDS             complement(5733..7148)
                     /gene="phoA"
                     /locus_tag="N7B39_004032"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HGU2423916.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTADQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(7249..7509)
                     /gene="iraP"
                     /locus_tag="N7B39_004033"
     CDS             complement(7249..7509)
                     /gene="iraP"
                     /locus_tag="N7B39_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HGU2423917.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
BASE COUNT         1845 a         1970 c         1789 g         1934 t
ORIGIN      
        1 tcgccggaaa tcgttaaatt accgttcacc accagtttat ttttattgct gaagttagcg
       61 ggtaatgata ggtcaccatt atgataatac cagcggatat tatcttttag catataagca
      121 gcttccatac tatcgtcggt tgccgcttta accacgttgc gctcatctgc cgtcataaga
      181 tgaaagtcag aggtgataag gtcctcgcta ctgatccact ctgtggcaca tgcattgcca
      241 gtcataaaaa ataagaccat acagatgacc aatatattaa ttctattcat aatcttcaat
      301 gatatttata tcaaccttga tgaattatga atatatcgga aattgaaaaa atcgcagcaa
      361 aggaaatatt attatggggt tatgagagga catttcagaa aaatgccctc ccgttcaagc
      421 ggaaggcgag gaattacaga tagcggcaca gataagaggt gggttcggca acttgcagat
      481 gaaactcact gtgaccggga acattaaaca ccgaaccggc ttcatacact tgccagtcgg
      541 tcgcgtcagg cagtaacaca ttcagcgcgc cactgattac cgtcatctct tccggctcag
      601 cggtgctgaa ggtgtattcg ccttcaacca taacacccac gctggcgcga ccagtgctgc
      661 tgctggaaaa gccgatcgat ttcactttgc cggaaaagta ctcattactt tgaagcataa
      721 actggccctg tctggtagtt gaatatctac ttcactatag gggccagatg cgctggctgt
      781 cacgcaaaat tacaccagta attccgcagc cagccgtgca atcaatacgt tagagagcaa
      841 gacaggaaca tcgagctgtt tttgcagtaa atcgcgatga cgctggttaa atcccaaaca
      901 atccagcatg atgacatctg cgcctttcgc cagtaattct ttcccggcat caatgatttt
      961 ttgttctgaa tcatgaatgg ggttacccaa tgaaaataca ggcggtttct gcaaaatttg
     1021 ccatttttgc gcctgaacgg tcagcaactc ctcaaccggt acgataaccc ccacctgatg
     1081 atcttcaaca atagaggaaa ccagcggtgg caatatccgc gacggctcaa gaaagatcgt
     1141 attacgcgca gtcatactac taatgtttgc cgtactcatt aatataatga cgtcgtagcc
     1201 ctgattatcg agcacttcaa ccacaccttg caggtcacgc tctactttgc gacgcgaaac
     1261 atgggccaga tggttgtcat ttaataatgt gagaattgtg tcttcgcctg cttctggcgc
     1321 gtactccgcc atcacttctt cgcggcttaa cttccccagc aggctatgat gggaaatatt
     1381 atcttcgtca atgtattctg tcaggagcgg caaaacttcc tgcataggta caatgccgat
     1441 ggttagaatc gccaacgacg cactcattgt tcttccacct tacttttatt tcgtcgttaa
     1501 ttaccgggca agtgttaagc accactgctg acacttactt gtgctacaaa tgacaaaaag
     1561 cgtagcagca gacgtgcacg gcataatgta aagattccaa atgattccag taatggattg
     1621 ttattgttta atattctaat tatttgaaaa acatgaatta tgaaaaatgt gacgcagatc
     1681 ccttgtagtg gcactcagaa tcccttccga gcagtctgat gcggtcactc tttgtcaggg
     1741 tcctcatagc gttttttcgc atccatcgct tcctgagcgg tcggatgttc actgatcaac
     1801 gagtctggtt taggatgatc ggctctgagt tggtaccagg ttaccgtctg tcctggcttt
     1861 cctttttcaa ttgtcactat atatgcttca cgaggataag gcggtttcgt tggcatcgtt
     1921 cttccttatt tcacgggatg aacgttaagt ataggcgctc gaaaatcaac aattgatcgt
     1981 ctgcgccagg gcgctgcgaa tttcagaaat cacctggctg ggttcgtttg ttgcgtcgat
     2041 gataatatgc gcaacttcgc gatatagcgc atcgcgttct tccagcactt cctgaacttc
     2101 ttcgctcagc ggttttcccg ttaaggttgg ccgtaaatct tcttccggtg cagcttgcag
     2161 tcggttaacc aggactgata ctggcgcaca caaataaacc acgatcccgt tattttgcat
     2221 gaagtgacga ttaaattccg tcagaataat gccgccgcct gtagcgataa cggtggatgg
     2281 cgcagttacc gcttccagcg ccgccgtttc tctggcgcga aatcccgccc actcttccct
     2341 ttcgacgatc tccgcgaccg tcatattgag ctgtgattgc aaccactgat cggtatcgac
     2401 aaaccgacgg ttaagcgaat cggcaagggc cattccgacc gttgttttac cacagccccg
     2461 aggcccgatc agaaaaagag gttgtgtcat cgtgggtttt ccccaatagg tcgcaatgcg
     2521 gcgaaagccg gtgtcatgag aatagcgatc ataccatcaa actagtacaa tttcgattgt
     2581 aaagaaaaaa ttccacttaa agtgaaaatc tcaatacatc ccttactata ccaataaata
     2641 ttcaagaatg aagtgtaaat aataaattac atttagccac gactacgttg cacttccagc
     2701 caccacttct ccagctccgc ggcaaaggcc tggcggtcac gttgtgaaag gctatctggt
     2761 ccgccggtct ggatcccact ggcacgtaag gtatccataa aatcgcgcat cgtcaggcgc
     2821 tcacgaatgg tcgctggcgt gtaacgttca ccgcgcggat taagcgccgc agcgcctttc
     2881 tcgatggctt cagcagccaa aggtatatct gcggtgatca ccaaatctcc cgcttcacac
     2941 tgccggacaa tttcgttatc ggcaacgtcg aaacctgccg cgacgcgcag cgtacgaata
     3001 aatcgcgatg gcggcacgcg taaactctgg tttgctacca gtaccagcgg catctgcata
     3061 cgttccgccg cgcgatacaa aatctcttta attacattgg gacacgcgtc ggcatccacc
     3121 caaattgtca taaagtcatc ctttgttggg taatcctcta ttgtgtcgcg cttttgcctt
     3181 ccggcatagt tctgtttatg cttctgccag cgattatcaa aacaatgaat ttcacggcag
     3241 gagtgaggca atggaaaaga aaatcggttt tattggctgc ggcaatatgg gaaaagccat
     3301 tctcggcggt ctgattgcca gcggtcaggt gcttccaggg caaatctggg tatatacccc
     3361 ctccccggat aaagtcgccg ccctgcatga caagttcggc atcaacgccg cagaatcggc
     3421 gcaagaagtg gcgcaaatcg ccgacatcat ttttgccgcc gttaaacctg gcatcatgat
     3481 taaagtgctt agcgaaatca cctccagcct caataaagac tctctggtcg tttctattgc
     3541 tgcaggtgtc acgctcgacc agcttgcccg cgcgctgggc catgaccgga aaattatccg
     3601 cgccatgccg aacactcccg cgctggttaa tgccgggatg acctccgtaa cgccaaacgc
     3661 gctggtaacc ccagaagata ccgctgatgt gctgaatatt ttccgctgct ttggcgaagc
     3721 ggaagtaatt gctgagccga tgatccaccc ggtggtcggt gtgagcggtt cttcgccagc
     3781 ctacgtcttt atgtttatcg aagcgatggc cgacgccgcc gtgctgggcg ggatgccacg
     3841 cgcccaggcg tataaatttg ccgctcaggc ggtaatgggt tccgcaaaaa tggtgctgga
     3901 aacgggagaa catccggggg cactgaaaga tatggtctgc tcaccgggag gcaccaccat
     3961 tgaagcggta cgcgtactgg aagagaaagg cttccgtgcc gcagtgatcg aagcgatgac
     4021 gaagtgtatg gaaaaatcag aaaaactcag caaatcctga tgactttcgc cggacgtcag
     4081 gccgccactt cggtgcggtt acgtccggct ttctttgctt tgtagagcgc caaatctgcc
     4141 gatttcaacc actcgcgata gtgactcatt tgtgggttca gcggcgcaac ccccacacta
     4201 atccgtaaat ttacctgtgg cgtattcggc aaacgtaatg tatttagccc ttcatgcacc
     4261 cgtaacatgg cggtaatggc gctctcagct ggcgtaccgg acatgattac cgcaaactca
     4321 tcgccgccaa accgaccaat cacatcgcta ccgcgcaggg taatttgtaa ctgtcgggta
     4381 agcgccacaa tcgcttcatc gcccacatca tggccccagg tatcgttgat gctcttgaaa
     4441 tggtcgatat cgataatcag taacgttgca tcgcgattat gccgccgaca gttatcaaat
     4501 tcattgcgta acatagtttc ccaatgacgt cggttataca cgcccgtcat gccgtcgcgg
     4561 gtactcatga cctgcaacct gcgtttatgt tccgccagtt tggttgccgt ctggtagctg
     4621 acccagccaa acagcagagg ataaatgaca ataatgggaa gggagagcca ccattccagc
     4681 ggcgcactat tgaacgacac ggtaatgccc gtcagctcga gggtgacaag gcaggaaacc
     4741 accatcaaca ccagacccgc gacaaacaga cgggggccgc ctgcccccat caaattcaga
     4801 cacataatca tcaacatcgc ggtggaaggc agcacgttta cgcccattac gcctacccac
     4861 attcccgcta atactgcatc ggtttttaag ttgtaaattt cccggctaag cggatcgacg
     4921 gccctgctcg ctatctgcca ggctaaatgc ggccagacga acgcccagcc gaccaacacc
     4981 agccaccacc agcccggcgg cgggtgtgaa accagcgttg aagcaatcgg taagaacatg
     5041 ccagccaggc caaccgcacg gggtagtctg acgcgacggg cgaagcgcag cccggaccgc
     5101 tgatgttcgt tttgaggagt taaaggaggt tcctccccgt gcgccgccgc ttttttgaaa
     5161 aagttttcat cattcattat ttttgggaac attcagaaac aattttccca aattatagag
     5221 acggggctaa aggcagaagt atgaaatttc gggtgagcca ttttagtagc tcacccgtta
     5281 gcactatcag gtatcaggcg gctttcttga ggcaggcgct cataaactta ttacgatcat
     5341 cacctttcag tgattgttgc gttgcttgat tattacattc gcgcatcttt tgctgctgtg
     5401 gcgtcaaact tttttcacca ggcgcagact tgctgttctt caggcaatca ctcatgtagg
     5461 tcttacgagc atcccctttc aacgcctgtg ccgtcgcctg ctgattacag gaggtcatac
     5521 gctgttgttg tggggttaag gttctctcgg cagcgccgac ggtggttaaa aaaaccagac
     5581 cgaaaagcaa ggtaaccagt aatgtaattt tcatagcacc atccctcttc atgttttaac
     5641 catgagcgta tgcgcccgtg atctgacatt aagtctggtt gctaacagca aaaaaaccac
     5701 ccggcagcga aaattcactg ccgggagcgg ttttatttca gccccagagc ggctttcatg
     5761 gtgtagaaga gatcggtctg gtcggtcagt ccaacgacat tggcggcatg cgggccatac
     5821 gccgcaatac gcaactgact gccggtatgt tcttgtgaat cctcttcgga gttcccgtaa
     5881 ctcatcacca tcactgcgcc atctttggta tttagcgcct gagtgaggcc cggagctttg
     5941 gtatccggcg caacaatctg gctggcgtgg gcgtgatcag cggtgactat gaccagcgtg
     6001 ttaccatcct ttttggcgaa ttccagcgcc cgttgtacgg cttcatcgag atcgaccgtc
     6061 tcgccaattt gcccacaagg attcgcagca tgatcctgtt tatcgattga cgcaccttca
     6121 acttgcagga aaaagccttt ctcattttta ctcaacaatt caatggcttt gtcggtcatc
     6181 tgtgccaggg tcggtacact gtcattacgt tgcggattag gcgtacaggt gactgcgggc
     6241 ttatcgatat taccgtggta cgttgctttc ggtcctagcc agcgcactgg catattgccg
     6301 tcagcaaaca ggccaagcag gggtttttgc tgattcgctt ccgtcaccga attcagtgag
     6361 gcagcatcgc tcaccaactg ataaccacgc gcctgtgcct gttcacgcag cgtttttccc
     6421 tgccattcac cagcggttgc cgtttcagca aaggtttttg cgccgccgcc aagcgtaacg
     6481 tcggcacgag cgttaagcag ctgttcggta atcgatcctt ttccgccttt ttccagagcg
     6541 ttacccggac atttttcact ggtcgcgctc ggaccgtagc atttgcgcga ggtcacatgc
     6601 gccaccagcg cggcgggcgt ggcatcctgc aactctgcgg tagaaacgtt accggtcgcc
     6661 agacctgcgg cttttgccat ttccagaatc gttgggtgat ctttttcgtg aatatcgacg
     6721 cccagcgcgc cgttataggt tttgacaccg gttgaccagg cggttgctga tgcagccgag
     6781 tcggtgacgt agtccggttt tccggttttt ttattcagcg catagtgagt gtattgcccg
     6841 gtaagcggta aggcatcgat acctttaaaa aagccgcccg caccttcggc ataattacgt
     6901 gcggcagtaa tttccgaatc ccccatccca tcgccaatca gcaaaataat attttttgca
     6961 ggtttatcgc taagagaatc acgcagagcg gcggtctgat cagccgttaa gcggcgagca
     7021 ccgccgggtg cagtaatatc gccctgagca gcccggtttt ccagaacagg catttctggt
     7081 gtccgggctt ttgtcacagg ggtaaacagt aacggtaaga gtgccagtgc aatagtgctt
     7141 tgtttcactt tattttctcc atgtacaaat acattaaaaa ataaaaacaa agcgactata
     7201 agtctcggcc gtgacaactt tatgacagct gttgaaaaga ttaacttttt actgacgagg
     7261 atgcttcaat aacttcttta cgtaatcgcg cagcagctcc gtatcgtcgt caggaatgct
     7321 ggcatcgggc tttacctcgt acagcgcccc ctctacctga tcaatcaacc gctgttggtc
     7381 attttgcgcc atattgcgaa gcattgcagt gacgataatc tccaaagctt ctacctgcgc
     7441 acacagttct ttcgactctt cttctttttg ggcaagctta aataacaact cagcaatgag
     7501 atttttcatg tctgtatttc cttatccaaa gtatggag
//
LOCUS       DBFIFI010000204         7511 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.204, whole genome shotgun sequence.
ACCESSION   DBFIFI010000204 DBFIFI010000000
VERSION     DBFIFI010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7511
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.204"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            179..4432
                     /gene="fdeC"
                     /locus_tag="N7B39_004034"
     CDS             179..4432
                     /gene="fdeC"
                     /locus_tag="N7B39_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin FdeC"
                     /protein_id="HGU2423918.1"
                     /translation="MSRYKTGHKQPRFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMGNTTVTADNNVEKNVASFAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKDFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSGNDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDIEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAISAEVTYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLAKQLDTDSIRE
                     RRVLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTLSLGLVVSKATHGLK
                     NVQWEAPSLLAEGGKITGQGSQWQVTLPAYRPGKDNYYAISAVAYDNKGNTSKRVQTE
                     VVITGAGMSADRTALTLDGQSRIQMLANGNEQKPLVLSLRDAEGQPVTGMKDQIKTEL
                     TFKPAGNIVTRSLKATKSQAKPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDGMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDSEGNPVTGEASRLR
                     FVPQDTNGVTVGAISEIKPGVYSAAVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTVKDAYDNPVTSLTPEAPSLAGAAAEGSTASGW
                     TNNGDGTWTAQITLGSTAGELEVMPKLNGQNAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLVAKDAHGNAISGLALSASLTGTASEGATVSSWTEKGNGSYVATLTT
                     GGKTGELRVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKAPTVKTTTELTFTV
                     KDAYGNPVTGLKPDAPVFSGAASTGSERPSAGNWTEKGNGVYVSTLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIRDMTVKVNNQLANGQSANQITLTVVDTYGNPL
                     QGQEVTLTLPQGVTSKTGNTVTTNAAGKADIELMSTVAGEHNISASVNGAQKTVTVKF
                     NADASTGQANLQVDAAAQKVANGKDAFTLTANVEDKNGNPVPGSLVTFNLPRGVKPLT
                     GDNVWVKANDEGKAELQVVSVTAGTYEITASAGNSQPSNTQTITFVADKATATVSGIE
                     VIGNYALADGNAKQTYKVTVTDANNNLLKDSEVTLTASPANLVLTPNGTAKTNEQGQA
                     IFTATTTVAAKYTLTAKVSQADGQESTKTAESKFVADDKNAVLTASSDVTSLVADGIS
                     TAKLEVTLMSANNPVGGNMWVDIKTPEGVTEKDYQFLPSKNDHFVSGKITRTFSTSKP
                     GVYTFTFNALTYGGYEMKPVTVTITAVDADTAKGEEAMN"
     gene            complement(4553..5410)
                     /locus_tag="N7B39_004035"
     CDS             complement(4553..5410)
                     /locus_tag="N7B39_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU2423919.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIAISLHFDSQQSFSREFKKLFGCSPRV
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMKNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMPKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARVNGSDIERFYYNEDFVEKDSNDVVC
                     EIFIPVRPV"
     gene            5659..6528
                     /locus_tag="N7B39_004036"
     CDS             5659..6528
                     /locus_tag="N7B39_004036"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HGU2423920.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTREELFITSKLWVQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            complement(6688..7281)
                     /gene="rclC"
                     /locus_tag="N7B39_004037"
     CDS             complement(6688..7281)
                     /gene="rclC"
                     /locus_tag="N7B39_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HGU2423921.1"
                     /translation="MEKYLHLLSRGDKIGLALIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQGSNESSSTLKTEY"
     gene            complement(7293..>7511)
                     /gene="rclB"
                     /locus_tag="N7B39_004038"
     CDS             complement(7293..>7511)
                     /gene="rclB"
                     /locus_tag="N7B39_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HGU2423922.1"
                     /translation="LFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLEDAIADI
                     ARKYNASSWKVTSMRIDNNSTATAVLYK"
BASE COUNT         2043 a         1673 c         1938 g         1857 t
ORIGIN      
        1 ccacctgatg agtggctaca ggcactggat atattaggtg gcggtgtact ttcttacata
       61 aaggtatttc cttttctgcg gaaaaggaaa ccgggaaatc cccggttttt ctgacaagca
      121 gacgccatta tttgtgtctg cctatgttcg ttaattcgtt catcaggaaa ttatctcaat
      181 gtcacgttat aaaacaggtc ataaacaacc acgatttcgt tattcagttc tggcccgctg
      241 cgtggcgtgg gcaaatatct ctgttcaggt tctttttcca ctcgctgtca cctttacccc
      301 agtaatggcg gcacgtgcgc agcatgcggt tcagccacgg ttgagcatgg gaaatactac
      361 ggtaactgct gataataacg tggagaaaaa tgtcgcgtcg tttgccgcaa atgccgggac
      421 atttttaagc agtcagccag atagcgatgc gacacgtaat tttattaccg gaatggccac
      481 cgctaaagct aaccaggaaa tacaggagtg gctcgggaaa tatggtacag cgcgcgtcaa
      541 actgaatgtc gataaagatt tctcgctgaa ggattcttcg ctggaaatgc tttatccgat
      601 ttatgatacg ccgacaaata tgttgttcac tcagggggca atacatcgta cagacgatcg
      661 tactcagtca aatattggtt ttggctggcg tcatttttca ggaaatgact ggatggcggg
      721 ggtgaacacc tttatcgacc atgatttatc ccgtagtcat acccgcattg gtgttggtgc
      781 ggaatactgg cgcgattatc tgaaactgag cgccaatggt tatattcggg cttctggctg
      841 gaaaaaatcg ccggatattg aggattatca ggaacgcccg gcgaatggtt gggatatccg
      901 cgcagagggc tatttacctg cctggccgca gcttggcgca agcctgatgt atgaacagta
      961 ttatggcgat gaagtcgggc tgtttggtaa agataagcgc cagaaagacc cgcatgctat
     1021 ttctgccgag gtgacctata cgccagtgcc tcttctgaca ctgagcgccg ggcataagca
     1081 gggcaagagc ggtgagaatg acactcgctt tggcctggaa gttaactacc gaattggcga
     1141 acctttggcg aaacaactcg atacggatag cattcgcgag cgtcgggtac tggcaggcag
     1201 ccgctatgac ctggttgagc gtaataacaa catcgttctt gagtaccgca aatctgaagt
     1261 gatccgtatt gctctgcctg agcgtattga aggtaagggc ggtcagacac tttccctggg
     1321 gcttgtggtc agcaaagcaa ctcacggact gaaaaatgtg cagtgggaag cgccgtcatt
     1381 actggctgaa ggtggcaaaa ttaccggtca gggtagtcag tggcaagtaa cgctcccggc
     1441 ttatcgtcca ggcaaagaca attattatgc gatttcagca gttgcctacg ataacaaagg
     1501 caatacctca aaacgcgtgc agacagaggt ggtcattacc ggagctggta tgagcgccga
     1561 tcgcacggcg ttaacgcttg acggtcagag ccgtattcaa atgcttgcta acggtaatga
     1621 gcaaaaaccg ctggtgctgt ctctgcgcga cgccgagggc cagccagtca cgggcatgaa
     1681 agatcagatc aagactgaac taactttcaa accggctgga aatattgtga ctcgttccct
     1741 gaaggccact aaatcacagg caaagccaac actgggtgag ttcaccgaaa ctgaagcagg
     1801 ggtgtatcag tctgtcttta ctaccggaac gcagtcaggt gaggcaacga ttactgttag
     1861 cgttgatggc atgagcaaaa ccgtcactgc agaactgcgg gccacgatga tggatgtggc
     1921 aaactccacc ctgagcgcta acgagccgtc aggtgacgtg gttgctgatg gtcagcaagc
     1981 ctatacgttg acgttgactg cggtggactc cgagggtaat ccggtgacgg gagaagccag
     2041 ccgcttgcga tttgttccgc aagacactaa tggtgtaacc gttggtgcca tttcggaaat
     2101 aaaaccaggc gtttacagcg ccgcggtttc ttcgacccgt gccggaaacg ttgttgtgcg
     2161 tgctttcagc gagcagtatc agctgggcac attacaacaa acgctgaagt ttgttgccgg
     2221 gccgcttgat gcagcacatt cgtccatcac cctgaatcct gataaaccgg tggttggggg
     2281 gacagttacg gcaatctgga cggtaaaaga tgcctatgac aaccctgtga ccagcctcac
     2341 gccggaagcg ccgtcattag cgggtgccgc tgctgaaggt tctacggcat cgggctggac
     2401 aaataatggt gatgggacgt ggactgcgca gattactctc ggctctacgg cgggtgaatt
     2461 agaagttatg ccgaagctaa atggacagaa tgcggcagca aatgcggcaa aagtaaccgt
     2521 ggtggctgat gcgttatctt caaaccagtc gaaagtctct gtcgcagaag atcacgtaaa
     2581 agccggcgaa agcacaaccg tgacgctggt ggcgaaagat gcgcatggca acgctatcag
     2641 tggtcttgcg ttgtcggcaa gtttgacggg gaccgcctct gaaggggcga ccgtttccag
     2701 ttggaccgaa aaaggtaacg gttcctatgt tgctacgttg actacaggtg gaaagacggg
     2761 cgagcttcgc gtcatgcctc tcttcaacgg ccagccagca gccaccgaag ccgcgcagtt
     2821 gacggtcatc gccggagaga tgtcatcagc gaactctacg cttgttgcgg acaataaggc
     2881 tccgaccgtc aaaacgacga cggaactcac cttcaccgtg aaggatgcgt acgggaaccc
     2941 ggtcaccggg ctgaagccag atgcaccagt gtttagcggt gccgccagca cggggagtga
     3001 gcgtccttca gcaggaaact ggacagagaa aggtaatggg gtctacgtgt cgaccttaac
     3061 gctgggatct gccgcgggtc agttgtctgt gatgccgcga gtgaacggcc aaaatgccgt
     3121 tgctcagcca ctggtgctga acgttgcagg tgacgcatct aaggctgaga ttcgtgatat
     3181 gacagtgaag gttaataacc aactggctaa tggacagtct gctaaccaga taaccctgac
     3241 cgttgtggac acctatggta acccgttgca ggggcaggaa gttacgctga ctttaccgca
     3301 gggtgtgacc agcaagacgg ggaatacagt aacaactaat gcggcaggta aagcggacat
     3361 tgagcttatg tcaacggttg cgggagaaca caatatttcc gcttcggtga atggtgctca
     3421 gaagacggtc acggtgaaat tcaacgcgga tgccagcacc ggtcaggcaa acctgcaggt
     3481 agacgccgct gctcaaaaag tggcaaacgg caaagatgcc tttacgctga cggcgaacgt
     3541 tgaggataaa aatggtaacc ctgttccagg gagcctggtg acctttaatc tgccccgggg
     3601 tgtcaagccg cttacaggcg ataatgtctg ggtgaaagcc aacgatgagg ggaaagcaga
     3661 gttgcaggtg gtttcagtga ctgccggaac gtatgagatc acggcatcgg cagggaatag
     3721 ccagccttcg aatacgcaga ctataacgtt tgtagccgat aaggctaccg caaccgtctc
     3781 cggtattgag gtgattggca actatgcact ggcggacggc aatgccaaac agacgtataa
     3841 agttacggtg actgatgcca ataacaacct gttgaaagat agcgaagtga cgctgactgc
     3901 cagcccggca aatttagttc tgactcccaa tgggacggcg aaaactaatg agcaaggaca
     3961 ggctattttc accgccacga ccactgtcgc agcgaaatat acactcacgg cgaaagtgag
     4021 tcaggccgac ggtcaggaat cgacgaaaac tgccgaatct aaattcgtcg cggatgataa
     4081 aaatgcagta ctcaccgcat catctgatgt gacttctctg gtggcggatg ggatatcgac
     4141 tgcgaagctg gaggtgacac tgatgtcggc aaataacccc gttgggggga atatgtgggt
     4201 cgacattaag acgccagaag gggtgacgga gaaggattat cagttcctgc cgtcgaaaaa
     4261 tgaccatttc gtgagcggaa aaatcacgcg tacatttagt accagcaagc ctggtgtcta
     4321 tacgttcaca tttaacgccc tgacgtatgg cgggtacgaa atgaagccag tgacggtgac
     4381 cattaccgcg gtggatgccg atacggcaaa gggcgaggag gcgatgaact aatattaaga
     4441 atatggaagt aacattctaa taaacaataa tggcgttgac atcttcaacg ccattattta
     4501 ttgaattgcc tgacagtagt ctcgttataa gttcatttag atatagcgac agctaaaccg
     4561 gacgaacagg gataaatatt tcgcaaacaa catcattgct atccttttct acgaaatctt
     4621 cattgtaata aaaccgctca atatcactgc cgttcactct ggctaacccc ctggcgggta
     4681 attccatcag atagaggttt cgcgcccatt cagggtagtg ctcccactca ccttcatagc
     4741 ggaaagaggc atacagtcct cggttaattg tccactcttt cggcatctct gaatcggttt
     4801 gtttgcatac cgtaccggca aaagtttcaa ctgtgagtaa atcgaccgat tttgttgacg
     4861 gttcaaacgt ggagacgaaa tagatatccg tcttaaaatt cttcatacat ctggctaaat
     4921 gatgacgtcg cagtttgact tcttcatccg gtgatttatt cgacacttca atatcatatt
     4981 taaatgaatt gccaaaaata ggtgtctcag gaaagttgac taatctacac tccgtctttt
     5041 gctgttgacg tattaaaaaa gaagggaaga tatttgcgag atcccaataa tcacggtggc
     5101 ggtatacacg gggagagcag ccgaataact ttttaaattc gcggctgaat gactgctgtg
     5161 aatcaaaatg caaagagatt gcaatatcaa gcatagattt cgcggtgagc cggacaagaa
     5221 tggcggcacg acaaagcctt cgtttgcgaa tgtattctcc caaaggcaca tgcatgaaat
     5281 ttcggaacag aagctggatg ttgcgtctgc tgtagccaga tttctgtgcg atatcttcga
     5341 ttgaaatagg gtgctcaaga ttgcactcaa tccactccag gagctgctgt agaatcttct
     5401 gcctgatcat cacattctcc gttaattctt tgtcatatcg cacattttcg ttatgccctg
     5461 ggccctaagt gtatcaaaaa cagcaagggc gaaggcaaaa gcactggtag cgtctgcttc
     5521 gttgtccccg aaaatagaga ggtttcagtc ctacattatt aatgaatttt ttgcataagt
     5581 gatatcaaaa tccacatact aatttgaggt tacgttttaa cgtagactca ttgttcatgc
     5641 ctaatggagg gactgacagt ggaattttcc gtattaagta acaatctgaa aatgccgatg
     5701 atgggatttg gtgtttttca ggttaccgat aaaaacgtgt gcaaacagtc agtgttgaac
     5761 gctatccgca cggggtatcg actcatcgac actgcagcgg tatacggtaa tgaagatgcc
     5821 gtcggtgaag ccgttcgtga agctatatcc gaaggtttat gtacccgaga agagttattt
     5881 ataacgtcta aattgtgggt gcaggatatg ttgaatcagg atacggcagc agcaggtatt
     5941 gaagcatcat taaaaaaatc gggactagaa tacttcgacc tctatttatt gcatcaggct
     6001 atgcgcgatt atttcagtgc gtggcgtgca ctcgaagatg cctatgaaga gggcaaatta
     6061 aaagcgattg gggtttccaa tttctatcct catgttctgg cgaacttttg tgaaacggta
     6121 agagttaaac cgatggtcaa ccaggtcgag ttgcatccct attttgccca accagaggcg
     6181 ctggcaacca tgaagtatta taacgtgcag cctgaagcat gggctccgtt aggtggtgga
     6241 cgacataaac cctttgaaaa taatctgctt cagagtattg cagatgccca tcaaaaatcg
     6301 atttctcaag tcattctgcg ttggaatatt caacggggag tggtcgttat tccgaaatcg
     6361 acacatcaac agcgtatcga agaaaatttt gctatctggg atttctcact gacagagaaa
     6421 gaaatggcac aaattagttc gcttgatttg ggttatgttg gggagtcggt aaaacatttt
     6481 aatcctgaat ttgttcgtgg ttgtcttgct gtaaaaatac atgattgata ttaatcatgt
     6541 attttacctg agacgacaag aatcttttaa caggggagtg atattgatct tcactctgtc
     6601 atatctccgg taatatggcg tcaggcttca tcatcagaaa gggggcctga cgcctgaaaa
     6661 agtgaacaac agacagtgtt cggattatca atattcagtt tttaacgttg aactggattc
     6721 attactgcct tgtttaagaa tttcccgcgc cgaatctgcc attatcatga cagcacctgc
     6781 cagcatcaga gtatctttca ataccagacg accagcaccg gataaataag ggaaaccatg
     6841 atgagcgtca cccaatgcgg gtacccatgc ctccggggtg gtgattaaaa atgagagtgt
     6901 taccagcggt gtggtaaatg ccatcagccc tcccaataaa cctaaccagc gattgacagg
     6961 attagccaaa accagcagag caataatcac ctccacgacg ccaagaccgt tggaaaaacc
     7021 ataggtatta ttggccgatt gccatgccct tgcttctggt ttgtattcgc cttcgtgagt
     7081 cagatactgt ttatagtctt ccgggtgttc ataaaagaac gacattagtg gactgtttgc
     7141 gacgaatggt gtaatgctgt ctgcctcgta agggacaaac tttaataacc caatccacat
     7201 aaaaacaatc gcaatactca gacgaatcaa tgccaggcca attttatctc cccgacttaa
     7261 caggtgcagg tatttttcca ttgtgaatat ccttatttat acaatactgc tgttgcggtt
     7321 gaattattat caattcgcat cgatgtgact ttccaggatg aagcattata ttttctggct
     7381 atatcagcaa tggcatcctc cagaactgtc ggtgatgctg atactgacga gacgctgata
     7441 tgtttcagaa ttattttatc atctgcattg gtggaaaatg ccataacgcc agataatagt
     7501 gttgcaaata a
//
LOCUS       DBFIFI010000205         7468 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.205, whole genome shotgun sequence.
ACCESSION   DBFIFI010000205 DBFIFI010000000
VERSION     DBFIFI010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.205"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            190..420
                     /gene="chaB"
                     /locus_tag="N7B39_004039"
     CDS             190..420
                     /gene="chaB"
                     /locus_tag="N7B39_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HGU2423923.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            581..1276
                     /gene="chaC"
                     /locus_tag="N7B39_004040"
     CDS             581..1276
                     /gene="chaC"
                     /locus_tag="N7B39_004040"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HGU2423924.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     RPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(1320..1673)
                     /gene="ychN"
                     /locus_tag="N7B39_004041"
     CDS             complement(1320..1673)
                     /gene="ychN"
                     /locus_tag="N7B39_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HGU2423925.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLALRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            1859..3253
                     /gene="ychO"
                     /locus_tag="N7B39_004042"
                     /gene_synonym="iatA"
     CDS             1859..3253
                     /gene="ychO"
                     /locus_tag="N7B39_004042"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HGU2423926.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(3254..3904)
                     /gene="narL"
                     /locus_tag="N7B39_004043"
     CDS             complement(3254..3904)
                     /gene="narL"
                     /locus_tag="N7B39_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HGU2423927.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(3897..5693)
                     /gene="narX"
                     /locus_tag="N7B39_004044"
     CDS             complement(3897..5693)
                     /gene="narX"
                     /locus_tag="N7B39_004044"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HGU2423928.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            6032..7423
                     /gene="narK"
                     /locus_tag="N7B39_004045"
     CDS             6032..7423
                     /gene="narK"
                     /locus_tag="N7B39_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HGU2423929.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
BASE COUNT         1730 a         1828 c         1975 g         1935 t
ORIGIN      
        1 gtaacatttt acgaattttt accccgttgt cgatttttta ctcattgggg cataaaaata
       61 agtattacgt ttaggcaatg tttgttttag cgtttcttaa gaacagtctg acatgaaaat
      121 tcttatgttt ttgcaagagt agatattgtt gaccacactt aatgttcaac tttgtaaaag
      181 gagtcaacga tgccgtataa aacgaaaagc gacctgccgg aaagcgtaaa gcacgttcta
      241 ccgtctcatg cccaggatat ctataaagaa gcgttcaaca gcgcatggga tcaatataaa
      301 gataaagaag atcggcgtga tgacgccagt cgcgaagaaa ccgcgcataa agtggcctgg
      361 gctgctgtga agcatgaata tgccaaaggg gatgatgata aatggcataa aaaatcgtaa
      421 aaccggtagc ttagttaaag ctattcgtgc ggtgttgcct tgcaagtggt ccgcggattg
      481 catattgtcc cgttagtggt ttcaaaatga gcagtaaaaa tgtccggaag acaccaaaaa
      541 gttgtcgcag ggaagcatgc agtggcggag gtgtaagaag gtgataacgc gtgatttctt
      601 gatgaatgcc gattgtaaaa cggcatttgg tgccattgaa gaatcactct tatggtcagc
      661 agaacaacgg gcggcttcgc tggcggcgac gctggcttgt cgacctgatg agagaccggt
      721 gtggatcttc ggttatggat cgttgatgtg gaatccggca ctggagttta ccgagtcgtg
      781 caccggtaca ctggttggat ggcatcgcgc gttttgcctg cgcctgaccg ccgggcgcgg
      841 gactgcgcac cagccgggac ggatgcttgc actaaaagag ggcggacgta ccacaggcgt
      901 cgcctatcga ctgccagaag agacgctgga gcaggaacta accctgttgt ggaagcgcga
      961 gatgattacc ggctgttatc tgccaacctg gtgtcagctt gatcttgatg atggacgcac
     1021 agtaaacgcc attgtgttta ttatggaccc gcgacatcca gaatatgaat ctgacactcg
     1081 cgctcaggtc atcgcgccgt tgattgcggc ggcgagcggt ccgctgggaa ccaacgcaca
     1141 atacctgttt tcactggaac aggagcttat caaactggga atgcaggacg atgggctgaa
     1201 tgatttgctg gtatcggtaa aaaaactgct ggcggagaat tatccggatg gtgtgttgcg
     1261 gccgggattc gcctgagtaa acttccctca tcgtggggcg tcagacgccc cctcaaacat
     1321 taaaatgtga gcactttatc ggctgacagc gtccattgcg ccagttccac aagagtaccg
     1381 atttccaccc catcaatcag aggaagtgta ctaatcccgc gcccgtcggt acaggttttg
     1441 cacaatttca ccggtacatt ctgagcggta aggatctcca gcatttgctg tatgttgtag
     1501 ccttcccctg gtttttgccc gcgcaacccg gctgtgaccg catcagacat caggaacaga
     1561 cgcagagcca gattgctctc ctgctctcgt aacgcaatgg ccagccgcaa gctgttaaac
     1621 aaggattcgc tcccgtaagg tgcgccattg gcaacgatca cgattttttg cattatttac
     1681 tcctgtattc agggaattag acactcatct tctatcttac tgcttctgca gcgtctgacc
     1741 aatcggtcac atttttaagg attttcctga aagcgcgaga aaatacgaca aaagttgcca
     1801 gtaatcgtta ttctttaagg ctatgttttt tcatttttta ccggaagtta ccgacgtttt
     1861 gagccgtttc gttcctcgca ttattccgtt ttatttactc ttgctggcgg caggcggtac
     1921 agctaacgca caatctacct tcgagcaaaa agcggcaaat ccctttgata ataacaatga
     1981 tggtctgccg gatttaggca tggcgcctga aaatcatgat ggggaaaaac actttgctga
     2041 aattgtgaaa gatttcggcg aaaccagtat gaatgataac gggctggata ctggcgagca
     2101 ggcaaaagct ttcgcattgg gaaaagtccg cgacgcgctt agtcaacagg ttaatcagca
     2161 cgtagagtcg tggctatcac cgtggggaaa tgccagtgtt gacgtcaaag tggataacga
     2221 aggtcatttt accggcagtc gtggaagctg gtttgtgccg ttacaagata atgatcgtta
     2281 tctcacctgg agccagcttg gtcttactca gcaggatgat gggctggtga gcaatgtggg
     2341 cgttgggcaa cgctgggcgc gcggcaactg gctggtgggt tataacactt tttatgacaa
     2401 cttgctggat gaaaatcttc agcgagcggg ctttggtgcc gaagcgtggg gcgaatattt
     2461 gcgactatcg gcaaactttt atcagccgtt tgctgcatgg catgaacaga cagccacgca
     2521 ggaacaacgg atggcgcgcg ggtacgacct gacagcccgg atgcgcatgc cgttctatca
     2581 acacctcaat accagtgtca gcgtagaaca gtattttggt gatcgtgttg atttgtttaa
     2641 ctctggtacg ggttatcaca atcccgtcgc gttgagtctg ggattaaatt acacccctgt
     2701 gccattagtc actgtgacgg cccagcataa acagggtgaa agtggcgaga atcaaaataa
     2761 cctcgggctg aatcttaatt accgctttgg tgtaccgctc aaaaaacaac tttctgcggg
     2821 cgaggttgcc gaaagtcagt cgttacgtgg tagtcgctat gataatccgc agcgaaataa
     2881 tctaccgact cttgagtacc gacagcgaaa aacgttaacg gtgtttcttg cgacaccgcc
     2941 gtgggatcta aaacctggcg aaacagtgcc gctgaaatta caaatccgca gtcgttacgg
     3001 tattcggcaa ctgatttggc agggcgatac gcagatatta agtttgacgc cgggcgcaca
     3061 agccaacagc gcggagggct ggacgctgat catgcctgac tggcagaacg gggaaggggc
     3121 gagcaatcac tggcgattgt cggtggtggt ggaagataac caggggcagc gtgtctcctc
     3181 caatgagatc acgctaacgc ttgtcgaacc gttcgacgca ttgtcaaacg acgaactgcg
     3241 ctgggaaccg taatcagaaa atgcgctcct gatgcaccca taccgctgct tccacgcgag
     3301 acttgagctt cattttcttc agcatgtgct tgacgtgcac ttttactgtg ctttcggtga
     3361 tgtccaggcg gcgggcaatc atcttgttcg gcaaaccctg ggcaatcagc ttgagaatat
     3421 cgcgctcgcg tggggttaac tggttaacat cgcgctcagt cgtggcacgg ttagcgcgca
     3481 agctggcggc cagaacaggc gttaatgctt cgcttaatac catttcgcca gcagctgcct
     3541 gatgcaatgc tttcagcaga tcttccggtt ccatatcttt taacagatag ccatccgcgc
     3601 cgcgtttcag tgcggtgacc acatcttctt catggttaga gacgctgaat accacaatgc
     3661 gccctgagag ggacttttcg cgcagtttat ccagcgtttc cagaccgttc atgccgggca
     3721 tattgagatc taacaggatc agatcgggat caagagattc cgccagttca ataccctgtt
     3781 cgccattact cgcttcgcca accacggtga tatctggtgc catactgata agctgtttta
     3841 cgccagttcg cagcatcggg tgatcgtcaa tcagcaggat agtagccggt tcctgattac
     3901 tcatgggtat ctccttggac gtctgtgaaa gttttttcgg gaataaaggt gaccaccact
     3961 tcggtgccac ctgattcacg acggcggacg cggcaatcgc ctcgtaaact ttgcgcacgg
     4021 tcgcgcatta ttatcatgcc gtagtgattg ctgcggatgg cattttcagg cacgccgcag
     4081 ccgttatcct ggacggtcag tttgacctga ttatcgtttt gcgccaccgt caccacgacc
     4141 tcactcgctt gcgaatgttt gagggcgtta cttaatgcct cacgggcaat ttgcaacaag
     4201 tggattgcct gatgcgaagg caccagacga ggcggcaatt gataatccag cttcaccggg
     4261 aagccaaatt tggcgctgta ctcttcgcaa ctcgcctcca gcgccggacg taatccaggc
     4321 tcggtgagct gcaagcggaa tgtggtgagc aattcacgca actgcgccca ggatgcattc
     4381 agttcgttac ggatctgact taacagttcg cggctgcttt ctggcagcgc atcgccctgc
     4441 atctgtaaac aactcacctg catcttcatg caagagagag attgggcaat agaatcatgc
     4501 agttcgcgcg caatggtggc acgctcttcc atcacgatca actgttgctg acgttcctga
     4561 tgccgatcca gcgccagcgt ggcggtgagt tgttcaacca gggtatccac cagttgttgt
     4621 tgatcatggc taagatgacg cccctgcggt agggtcgcca gcaaaatacc gtactgcgta
     4681 tgagagtcag ccagccgcca cttcagggtc gtgccgcgat caccaacggg taatacgccg
     4741 cgcgggcaga gctggcagcc tttatcatca caagtcatat ctggctggca ggtaaactcc
     4801 tgatgattct cttcatcatc agtgtcatac acccgcagtt cgatatcacg tagcagggtt
     4861 aaattctgta agccgttgag tacaggtgac aggcgttcac acagcggggc gcgggaatgc
     4921 aaacggcggt tagcctgcca taaaaaagag aggatctgat ttttatgctc cagcccggcg
     4981 gttttctcct gaacccgctg ctcaagtacg gcataacttt cggccagttc tgcagacata
     5041 ttgttcaacg cagttccaag catcgccatt tcgttgcgcc cgctgatgtt tgcgcgttgg
     5101 gtaaaatcgc gatggctgac ggcactcgcc attgccagca gttgccgcca cggttgtagc
     5161 agtcgcgccc gcaaccagat aatagtgaac accagtaaaa gtgccataaa taccgccatt
     5221 acccgatgga ccagtaccac tgtctcaatg cgcatttccg tggtgcggtc aaaaccagat
     5281 accagctgat caagcccggc aacaaactgg ctgacatccg ctgacaccgt ttcgcggttt
     5341 tgtgcacgca tcagtgcagg gatgagttca ttacgccagt aatcttgtaa accctgtaat
     5401 tgcgccagtt gtccgtctcg ttctgctgct cgagtcaact cggcgctaaa tgccgtttgt
     5461 tccatctctt taattaaggg cttgtctttc tcgcttaatg gcactgctgc caacagacgg
     5521 taactttgca tgcgcagcga tcccgctttg ttgatcgcat gggcgctgcc ctgaacgcct
     5581 tgcaccagcc agccagaaac cgccatccct gccagtccaa tagcagtaga aagcaacaca
     5641 ataagcgcaa cctgattaac cagggtgagc ggagagagac aacgtttaag catgtaaacc
     5701 tcttccttca ggctttaaat gagcaataac cttaatgaat gtgacgatac attctggaat
     5761 ggcagtattc tcggctattg gctgaagtat acccataccc ggaaagagtt actccttatt
     5821 tgccgtgtgg ttagtagctt tacatcggta agggtaggga ttttacagca ccgtgaaaaa
     5881 tctcataatt tttatgaaat cactgtactc actatgggta atgataaata tcaatgatag
     5941 ataaagttat cttatcgttt gatttacatc aaattgcctt tagctacaga cactaaggtg
     6001 gcagacatcg aaacgagtat cagaggtgtc tatgagtcac tcatccgccc ccgaaagggc
     6061 tactggagct gtcattacag attggcgacc ggaagatcct gcgttctggc aacaacgcgg
     6121 tcaacgtatt gccagccgca acctgtggat ttccgttccc tgtctgctgc tggcgttttg
     6181 cgtatggatg ttgttcagcg ctgttgcggt gaacttaccg aaagtcggtt ttaattttac
     6241 gaccgatcag ctatttatgt tgactgcgtt gccttcggtt tctggcgcgt tattacgtgt
     6301 tccatactcc tttatggttc ctatcttcgg tggtcgtcgc tggacggcgt tcagcaccgg
     6361 tattctgatt attccttgtg tctggctggg ttttgccgtg caggatacct ccacgcctta
     6421 tagcgtcttc atcatcatct ctctgctgtg cggctttgct ggcgcgaact tcgcatccag
     6481 tatggcaaac atcagcttct tctttccgaa acagaagcag ggtggcgcgc tgggcctgaa
     6541 tggtggtctg ggcaacatgg gcgttagcgt tatgcagttg gttgctccgc tggtggtatc
     6601 actgtcgatt ttcgcagtat ttggtagcca gggcgtcaaa cagccggatg ggactgagct
     6661 gtatctggcg aatgcgtcct ggatatgggt gccgttcctt gccatcttca ccattgcggc
     6721 gtggtttggc atgaacgatc ttgctacctc gaaagcctcc atcaaggagc agttgccggt
     6781 actcaaacgg ggtcatctgt ggattatgag cctgctgtat ctggcaacct tcggttcctt
     6841 catcggcttc tccgcgggct ttgcgatgct gtcaaaaacg cagttcccgg atgttcagat
     6901 tctgcaatac gctttcttcg ggccgtttat tggtgcgctg gcgcgttctg caggtggtgc
     6961 attatctgac cgtctgggcg gaactcgtgt cacgctggtg aactttattc tgatggcgat
     7021 tttcagcggc ctgctgttcc tgaccttacc gactgacggg cagggcggaa gcttcatggc
     7081 gttcttcgcg gtcttcctgg cgctgttcct gacagctggg ctgggtagtg gttccacttt
     7141 ccagatgatt tccgtgatct tccgtaaact gacaatggat cgcgtgaaag cagaaggggg
     7201 ttctgacgaa cgtgcgatgc gtgaagcggc aaccgacacg gcggcggcgt tgggtttcat
     7261 ctctgcgatt ggcgcgattg gtggcttctt tatcccgaaa gcgttcggta gctcgctggc
     7321 attaacgggt tcgccagtcg gcgcaatgaa agtatttttg attttctata tcgcctgcgt
     7381 agtgattacc tgggcggtat atggtcggca ttctaaaaaa taaatcttaa tatttgatta
     7441 tcctttgcgc ggcatgatgt cgcgcttt
//
LOCUS       DBFIFI010000206         7386 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.206, whole genome shotgun sequence.
ACCESSION   DBFIFI010000206 DBFIFI010000000
VERSION     DBFIFI010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.206"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            150..392
                     /locus_tag="N7B39_004046"
     CDS             150..392
                     /locus_tag="N7B39_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HGU2423930.1"
                     /translation="MNSLAGIDMGRIILDLSNEVIKQLDDLEVQRNLPRTELLREAVD
                     QYLVNQSQTVRTSAFGIWQGCEEDGVEYQRKLREEW"
     gene            complement(722..1651)
                     /gene="fdhE"
                     /locus_tag="N7B39_004047"
     CDS             complement(722..1651)
                     /gene="fdhE"
                     /locus_tag="N7B39_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HGU2423931.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(1648..2283)
                     /gene="fdoI"
                     /locus_tag="N7B39_004048"
     CDS             complement(1648..2283)
                     /gene="fdoI"
                     /locus_tag="N7B39_004048"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HGU2423932.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2280..3182)
                     /gene="fdoH"
                     /locus_tag="N7B39_004049"
     CDS             complement(2280..3182)
                     /gene="fdoH"
                     /locus_tag="N7B39_004049"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HGU2423933.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNHADEEENNLHEEKDEERK"
     gene            complement(3195..6245)
                     /gene="fdnG"
                     /locus_tag="N7B39_004050"
     CDS             complement(3195..6245)
                     /gene="fdnG"
                     /locus_tag="N7B39_004050"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(5658..5660),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HGU2423934.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQGNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVISCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            6439..7272
                     /gene="fdhD"
                     /locus_tag="N7B39_004051"
     CDS             6439..7272
                     /gene="fdhD"
                     /locus_tag="N7B39_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HGU2423935.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         1668 a         1940 c         1968 g         1810 t
ORIGIN      
        1 tacttgaact tataataact gcaactgtta catcatatct ggaaaacgcc tcgcaaaaat
       61 aaaaaatgat gcgtaaatga gctttcttta aagattcgac aagggtactt ttctctccat
      121 agatatcaaa tgatattggt caaatattta tgaactctct ggcaggtatt gatatgggca
      181 gaattatact cgatttatcg aatgaggtga ttaagcaact tgatgatctt gaggtgcagc
      241 gtaatcttcc tcgtacggag ctattacggg aagcggtaga tcaatacttg gtaaatcaat
      301 cgcaaacagt aagaaccagt gcttttggca tatggcaagg gtgtgaggaa gatggtgtcg
      361 aataccagcg taagctgcgc gaggaatggt aatgggaaaa aatgatatca atcagattgc
      421 tgacaacgtg cgcgttgttc atgccggatg cggcgtgaac gccttacccg gcctacaaag
      481 tcgtataaat tcaatgtatt gcagtttatt ggtaggccta ataagcgcgg cgcatcaggc
      541 aatcttacgc ttatcatcag tctcataccc ataaccttat tgatgtgggg gtgggtaaat
      601 tgaggcaaaa caggcactgg cggcatagct tgaggtggta acgcggaata cgagagatgg
      661 tgtcgtcaca ccctatgagc tgtaatccgg gcgaccagcg ccacccggaa aacgtcagag
      721 attactcccc ttcacccgga aacaggaacg ggttgatgga actgcgggca tagccttctt
      781 gctccattcg cgcgtccagt accagagagg cgaggtcatc tgccacggct tcaactttcg
      841 gttctttttc ctgatagaga attttcaggt aagtgccgca gtcatcgcag ctttcggctt
      901 taatcgcggc ctgttcgtca tccagcgacc agtaatgcag tttgccgctc tgctcgcagt
      961 tgctgcattt tacgcgcaca acgtgccatt cagtttcaca caggttgcag tgcaggtaac
     1021 gtagcccctg agtggtgcca atttgcacca tgctggacac cggcatagaa ccacaaaccg
     1081 ggcaatattg acgttgctcg ccgtattcag cgcgggcttt gccggggatc agattggcca
     1141 tctgcgccca gtagagcgac agtgcagccc agataaacgg cgctttatcg ctgctgacgg
     1201 acgagaaatc agaggcaaac agtgcgctgg ccatatcttc cagctcctga gtcgatgcct
     1261 tctccagatt ctcaatcact gccagcgccg ggccgctcat ttcaggtttc agctcagcaa
     1321 tcagcgccat cagcagcttt tgccagtgct tatcacgcgg cagaacgtga atatccagcg
     1381 ggggcttgcc ttgtgcgctg gcttctttaa tgcgcgcagt cagatccatc tccagcggat
     1441 ggtcgtacag caccacttcc tgggcgtggg cgataagcgc agcaaagcgc aggtaatcac
     1501 ccagcggatt attttctgcc agctcgcgca gacgctcggc gcggcggttg tataaattct
     1561 tgagccgagg gaacaataac ggcggaatca tatccgccgt acgtttctcg ctcgaaccca
     1621 gctcatcttg cgggattatg cgaatactca ttcagctttc ttttccgttg tcttgcggac
     1681 ctcacggtac cagcgcgggt gatgtttctt cgcccaagcg ctggtaaccc atccttccac
     1741 catggcggta atcgtgcctt tcacccaaag ggcggcgtag atatgcacca tgataaccac
     1801 aattaacgcc actgcggcaa atgaatgcag cattaacgcg aatcggatca ccgggattga
     1861 gaaagcaggc gcaaaataag gacgccagat aatcacgccg ctcaccagca gcagaaccag
     1921 gaaaataatc gccgcccaga aaacgcattt ctgaccgaag ttataacgcc cggtgtcacc
     1981 tacttcctcg ttgacgacga tcttacgaat attcttcgcc caaaagatat catcccgatt
     2041 gattaggttg tgatgccagt aacggaaaaa catgatgatg aacgaggcaa acataaccac
     2101 gccgacaaac gggtgcagaa ttcgcgccag ctgcggtgtg cccatgattt gcatcaacca
     2161 gttgaaggac gggaacaaaa agcccagccc gctcaccgcc gccaggatga agcagaaggc
     2221 ggtgatccag tggttgatac gttccggcgc ggtgtagcgc acgatggtgt cacgtcgttt
     2281 catttgcgct cctcgtcttt ctcttcgtgc agattattct cttcctcatc cgcatggttc
     2341 ggaccgacac ccacgtagtg gaagatactg gctgcgaagg tagccgcaaa gccaacagct
     2401 gcgagcggtt tccagatgcc tttccagaat ttcacggttt cgctgatttc cgggttctcc
     2461 ggcaagccat gatacagatt tggcttgtca gcatggtgca gcacgtacat aacgtgtgta
     2521 ccaccgacgc ctgccggatc gtacaggccc gcattgtcgt aaccgcgggt tttcagctcc
     2581 gccacgcgct cgctcgccag cgttttcatc gactctttcg taccgaagtg aatcgcgccc
     2641 gttgggcagg tcttcacgca ggctggttct tgcccaacca ccacgcggtc aacgcacagc
     2701 gtacatttgt agacgcggtt gtcttccggg ttgaggcgcg gaatgtcgaa cggacagccc
     2761 gcaatgcaat aaccgcagcc aatgcactgc tcggactgga agtcgacgat accgttggca
     2821 tactgaataa ttgcaccttc cgccgggcac gctttcagac agcctggatc ggaacagtgc
     2881 atacagccgt ctttgcggat cagccattcc agtttgtcgt tctgctccac ttccgagaag
     2941 cgcatcaccg tccacgattt ggcgcttaaa tcattggggt tgtcgtacac cccaatgtta
     3001 ttgccgacgg tatcgcgaat gtcgttccac tctgaacacg ccacctgaca ggctttacag
     3061 ccgatacagg tggtgacgtc gatgagtttc gccacttctt cctggaagtc ccgcgcctga
     3121 ggcgcggggg tcagaccgtt agtcgcggaa cgacgaatga tatcttgaga ttgataagcc
     3181 atatgtcgtc tccgttacac cttttccaca ttcacaagga aggacttaaa ctccggcgtc
     3241 tgcgtgttcg catcaccgac gaatggcgtc aacgtattgg caataaagcc ttttttcgca
     3301 acaccttcat agccccagtg aataggaata ccgatggtat cgatatcttt gccgttcgct
     3361 ttcagcgtgc gaatacgttt ggtcaccacc gctttggctt tgatatagcc acggttggag
     3421 gagactttca cggtatcgcc ctgggcaatg ccaagtttat tcgccagcga ctccccgatt
     3481 tccacaaact gctctggttg caaaatcgcg ttcaacagcg cgtgtttggt ccagtagtgg
     3541 aagtgctcgg tcagacgata ggtggttccg acatacggga acttatcggc tttacccaat
     3601 gcttcggcgt cgtctttaaa gatacgtgca gccgggttcg agataacgtt tggatgcagc
     3661 gggttagttc ccagcggcgt ttcaaacggc tcgtagtgtt ccgggaacgg accttccgcc
     3721 atcttatcga gggcaaacag acgccccatg ccttcctgct gcatgataaa cggcccgacg
     3781 ccgctacccg gaggcgctgc gctgtagtcc ggaatatccc agccggtcca cttagtgccg
     3841 tcccatttca gcaactgacg cttcggatcc cacgggttac cctgcggatc tgcggaggcg
     3901 cggttataca gaatgcggcg gttaagcggc catgcccatg cccaacccag cgtgttaccg
     3961 aggccagacg gatcggcgtt atcacggcgt gccatctggt tgccttccgg cgtccagcta
     4021 ccggcgaaaa tccagcaacc acaggaagtt gtaccgtcat cgcgcagttg ggcaaacgag
     4081 ctaagttgtt ggcctttctt gacgataacc gcaccggttg ccggatcggt aatatcggcc
     4141 agcgccttac cgttgctctc catcgctact tcttccgact tcggctcatg cggaatggcg
     4201 tagttccagg tcatgttcag cacctggtcc gggttcgcgc caccctgttc ggcatacatc
     4261 ttgcgcaagc gcaggaagat accggagagg atctcgccat cagtcagcgc aatccccggg
     4321 gcgtccgcac ctttccagtg ccactgcaac cagcgacctg agttaacgat tgaaccgttc
     4381 tcttctgcga agcaggtcga tggcagacgg aacacttcgg tctggatctt cgacgagtca
     4441 acttcgttca gctcaccgtg gttctgccag aagttagagg tttcagtgtt cagcgggtcg
     4501 atggtgacga ggaacttcag tttcgacaga caactgatca ctttgttttt gttcgggaat
     4561 gaggcaacag ggttaaagcc ctggcagata tagccattga ccttgccttc tttcatcatc
     4621 tcgaagtatt gcaggacgtc gtagccttta tcccacttcg gcaaccagtc aaagccccag
     4681 ctattttctg ccgtcgcttt atcaccaaag aaggccttca tcatagagac gaagaatttc
     4741 gggtagttgc cccagtagtt tccctggcct tccagcagcg gttttggcgt gttggcggta
     4801 aggtaggttt gcagatcggt ctgcttctcg cttggcagcg tcatgtaacc tggcaggctc
     4861 tgcgacagca gccccaggtc cgtcagcccc tgaatattgg agtgaccgcg cagggcgtta
     4921 acgccgccgc cagccatccc catgttaccg agcagcagct ggatcatcgc catcgtacga
     4981 atgttttgcg caccaacgga gtgttgcgtc cagccgaggg catacaggaa cgaggcagtt
     5041 ttatcgtgag cactggtttc tgcgatgtat tcgcagactt tcaggaacgc gtcttttggc
     5101 gtaccacaga tgttttcaac cacatctggc gtgtagcggg aaacgtgctg tttcagcaag
     5161 ttccacacgc agcgcggatg ttgcagcgtg gtatcgcgtt tggcgaagcc gttttcgtcc
     5221 agttcataag tccaggagga tttatcgtac ttgcgttttt ccgcgtcgta gccggtgaac
     5281 aggccatctt caaagccgta atcctcacgc acgatcaggc tggcgttggt ataggcttca
     5341 gtgtattcgc ggttgaattt ttcattgttc agcaggtaca gcaatacgcc tgacaggaaa
     5401 gcaatgtcag taccggaacg aataggggca tagtagtcag ccaccgcagc cgtacgcgta
     5461 aagcgaggat cgatcacaat cagcttcgcg ccattgtgaa ttttggcttc catcgcccag
     5521 cggaacccga ccgggtgagc ttcagcggcg ttaccgccca tcaccacgac gaggttggcg
     5581 ttcttgatgt cgacccagtg gttggtcatc gcaccgcgac caaatgttgg agcaagactt
     5641 gctaccgttg gtccgtgtca gacacgcgcc tggttgtcga ccgcgagcat acccagcgcg
     5701 cgggagaatt tttgcgttaa atagccggtt tcgttactcg acgcggaagc acacagcatc
     5761 ccggtggaga gccagcggtt aacagtcacg ccttcggcgt tttgcgcaat gtagttagca
     5821 tcgcggtctt ctttcatcag tttggcgatg cgatcaaacg cctcttccca actgatttgc
     5881 tgccatttat cagaacctgg cgcacggtat tccggaaact tcagacggct ttcggagtgg
     5941 atgaaatcca ccaggccagc gcctttcgga caaagtgcac cgcggttgac cgggtgatcc
     6001 ggatcgcctt cgatatggaa gatagatgct ttggcgtttt ttgctccgtc accgaggctg
     6061 tacatcaaca gcccacagcc tacggaacaa taggtgcagg tattacgggt ttcgcgggtg
     6121 cgcagcagtt tatactgccg ggtttccgcg agtgctacgc tgggtgcaaa acccagtgcc
     6181 gctgccgtgg tgcctgccat accgccagcg cagatcttaa agaactgcct tctgctgacc
     6241 tgcatggatt gctccttgtt tcgacattgt cacgtcccat ttacattcgc ttgctgcgtg
     6301 tgcagggagt gggagttatt tttctttgcg gaaggggccg caaaggtcca gaattggctc
     6361 aatttccctc catccaggaa gggtttgtaa cagaatacca taatgttggt gtgtgtgttc
     6421 ttatctggtt aagagaaagt gaaaaaaaca cagcaaaaag aaatcgaaaa tgtgacaaat
     6481 atcacaggtg ttcgtcaaat tgagttatgg cggcgtgatg atttacaaca cccacggtta
     6541 gatgaggtcg cggaagaagt tcccgttgcg ctggtctaca acggcatttc gcatgtggtg
     6601 atgatggcgt cgcccaaaga ccttgagtac tttgcgctcg gtttttcgct ttccgagggg
     6661 ataatagaaa gtccgcgcga tatcttcggc atggatgtcg ttccttcctg taatggtctt
     6721 gaagtacaaa ttgagctttc cagccgccgc tttatggggt tgaaggagcg ccgccgggcg
     6781 ctggcgggac gtacgggctg tggcgtatgc ggcgtggagc aacttaatga catcggaaaa
     6841 ccggtgcagc cgctaccgtt cacccagacg tttgatctca acaaactgga tgatgcatta
     6901 cgtcatctca acgattttca gccagtggga caactgaccg gttgtactca cgccgccgcc
     6961 tggatgttgc catctggcga actggtcggc gggcatgaag acgtgggtcg ccatgtggcg
     7021 ctggacaaac tgttaggccg ccggtcacaa gaaggggaaa gctggcagca aggtgcggta
     7081 ctggtttcca gtcgtgccag ttatgaaatg gtgcaaaagt cggcgatgtg cggcgtagag
     7141 attttgtttg cggtgtctgc cgcgaccacg cttgctgtag aagtggctga gcgctgtaat
     7201 ctgacactgg taggtttttg taaaccgggt cgggcaacgg tttataccca tccgcagcgt
     7261 ttgagcaatt aattaaaata agtcctgcga tatatattaa attgcaggac ttattcattt
     7321 cgtgaatttt attattttat ttataaacaa aatgcgtgta gtggcgtttt ctttttttca
     7381 tttatt
//
LOCUS       DBFIFI010000207         7381 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.207, whole genome shotgun sequence.
ACCESSION   DBFIFI010000207 DBFIFI010000000
VERSION     DBFIFI010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7381
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.207"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            31..291
                     /gene="dinJ"
                     /locus_tag="N7B39_004052"
     CDS             31..291
                     /gene="dinJ"
                     /locus_tag="N7B39_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HGU2423936.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDKLGV"
     gene            294..570
                     /gene="yafQ"
                     /locus_tag="N7B39_004053"
                     /pseudo
     CDS             294..570
                     /gene="yafQ"
                     /locus_tag="N7B39_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
     gene            726..1466
                     /gene="dpaA"
                     /locus_tag="N7B39_004054"
     CDS             726..1466
                     /gene="dpaA"
                     /locus_tag="N7B39_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HGU2423937.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(1437..2204)
                     /locus_tag="N7B39_004055"
     CDS             complement(1437..2204)
                     /locus_tag="N7B39_004055"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HGU2423938.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGKTDSEKAFCWLLYKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(2309..2887)
                     /gene="lpcA"
                     /locus_tag="N7B39_004056"
     CDS             complement(2309..2887)
                     /gene="lpcA"
                     /locus_tag="N7B39_004056"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HGU2423939.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            3127..5571
                     /gene="fadE"
                     /locus_tag="N7B39_004057"
     CDS             3127..5571
                     /gene="fadE"
                     /locus_tag="N7B39_004057"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HGU2423940.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTLGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNVFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(5614..6060)
                     /gene="ivy"
                     /locus_tag="N7B39_004058"
     CDS             complement(5614..6060)
                     /gene="ivy"
                     /locus_tag="N7B39_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HGU2423941.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            6241..7011
                     /gene="yafV"
                     /locus_tag="N7B39_004059"
     CDS             6241..7011
                     /gene="yafV"
                     /locus_tag="N7B39_004059"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HGU2423942.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMVALREYREKFPAWRDADEFRLR"
     gene            complement(7129..>7381)
                     /locus_tag="N7B39_004060"
     CDS             complement(7129..>7381)
                     /locus_tag="N7B39_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HGU2423943.1"
                     /translation="LHWVLDVKMNEDASRIRRGNAAEIISGIKKMALNLLRDCKDIKG
                     GVKRKRKKVALNTCYIEEVLASCSELGFRTDKMKNLTQI"
BASE COUNT         1771 a         1862 c         1924 g         1824 t
ORIGIN      
        1 gaataaatat acagcatagg agatatccca atggctgcta acgcatttgt tcgcgcccga
       61 atcgatgaag atctgaagaa tcaggcggcg gacgtactgg ccgggatggg gctgaccatc
      121 tctgacctgg ttcgcataac cctcacaaag gtcgcgcgtg aaaaggcatt gccgtttgat
      181 ttacgcgagc ctaatcaatt aaccattcaa tcaatcaaaa acagcgaagc tggcgttgat
      241 gttcataagg ccaaagacgc cgatgattta tttgataaat taggagttta aatatgattc
      301 aaagggatat tgaactcggg acaattttca aaggatgtaa aacttgcaca aaagcgtcat
      361 aaggatatga ataaattgaa atatcttatg acgcttctta tcaataatac tttaccgctt
      421 ccagctgttt ataaagacca cccgctgcaa ggttcatgga aaggttatcg cgatgctcat
      481 gtcgaaccgg actggatcct gatttacaaa cttaccgata aacttttacg atttgagaga
      541 actggaactc acgcggcgct ctttgggtaa agcccgctgc cgatgaaatt tgtaaaccct
      601 gcgccgacag gcttgcgtaa aactcttttg gtgataatgt gagccttccc aatccagact
      661 attccgattt gggatccatt ttgccgtcct ggcgtgtgta accgttttat caaggaataa
      721 gcagtatgcg taaaatcgca ttaattcttg cgatgttgtt gatcccgtgc gtttcgtttg
      781 ccggtttgct gggcagcagt agctcgacca cgccagtgag caaagagtat aagcagcagt
      841 tgatgggatc ccctgtctac atccagatct tcaaggaaga acgtacgctc gatctctacg
      901 tcaaaatggg cgagcaatat caactgctcg acagctataa aatctgtaaa tattccggcg
      961 gcttagggcc aaaacagcgt cagggcgatt tcaaaagccc ggaagggttt tatagcgtcc
     1021 agcgtaatca gttaaaacca gacagccgtt actacaaagc gattaatatt ggtttcccca
     1081 atgcctatga ccgtgcgcat ggttatgaag ggaaatacct gatgatccac ggcgattgtg
     1141 tttccatcgg ctgctacgcg atgaccaatc agggcattga cgagattttc cagttcgtta
     1201 ctggcgcact ggtatttggt cagccgagcg tgcaagtgag catttacccg ttccgcatga
     1261 ccgacgccaa tatgaagcgc cataaatatt ccaactttaa ggacttctgg gagcaactga
     1321 agccaggcta cgactacttt gagcaaaccc gtaagccacc gaccgtttct gtggtcaatg
     1381 gccgttacgt ggtcagcaag ccgttaagcc acgaagttgt gcaaccacaa ctggcatcaa
     1441 actacacgct ccccgaggca aaataagcgc cattcgcctg gcataatctt ttgccaggtt
     1501 tcattgcccg tcagcggctg tgtcgcaatc accgtgacca catcattcgg tgtggtctgc
     1561 gagctgaagt cgatttccac atcctgatcc agcaacgttg ccacgccaaa cggcgcgcgg
     1621 cgggtgatcc agtgcagatt ggtcgagcaa tacgccatta catagcgccc atccgaaagc
     1681 agcatgttga aaacgccctt ctgccgcagt tcatccgcca gtgaggcgat atatttaaat
     1741 accgccgcca tattgcccgg cgtacgcgga taacgctgcg ttaatttata cagcagccag
     1801 caaaaggctt tttcgctgtc ggttttacct accgggcgga agttgccggt ttccagtgat
     1861 ttatagcccg tcagttgtcc gttatgggca taagtccagt tacgtcccca taactcgcgg
     1921 gtaaacgggt gagtattttc caacgccacc tcgccccgat ttgcctggcg aatatgagcc
     1981 acaaccgaac aggattttat cgggtagtcc tgaacaagtt tggcaatggg ggaattaaag
     2041 ctgggttgtg gatctttaaa tgtgcgacag cctttacctt cgtaaaaggt aatgccccag
     2101 ccatctttat gtggcccggt tccaccacca cgctgcacca gcccggtgaa actaaagcaa
     2161 atatcggtag ggacgttggc gctcatcccg agcaattcgc acatcggtaa cccccacaac
     2221 agcgggagcg catggccccc ggcgaagatt gacttaagaa ttttcaggcc ggatgcggcg
     2281 taaacgcatt attcggccta cgccagactt acttaaccat ctctttttca attaactgga
     2341 tcaggatatg gatcacttta atgtgaattt cctgaatgcg gtcggcataa ccaaaatgtg
     2401 gaacgcgaat ttcaatatcc gccgtaccag ccattttgcc gccatctttg ccggtcaggg
     2461 taatcacttt cattcccttc tcacgcgccg ccgcgatcgc tttgatcacg tttgcagagt
     2521 taccagacgt ggagatcccc agcagtacat cgccttcgcg gcctaccgct tcaacatagc
     2581 gggagaaaat atcattgaaa ccaaaatcgt taccgacgca ggaaatatgg ctgacgtcag
     2641 agatggcaat cgccgggtag cccggacggt tttcacggta gcgaccggtc agctcttcgg
     2701 caaagtgcat ggcgtcgcaa tgggagccgc cgttgccgca ggaaagcacc ttgccaccgg
     2761 ctttaaagct gtctgctaac aggaccgccg ctcgctgaat ggcgtgaata ttggcgtcat
     2821 cttttaaaaa gttagccagc gtttccgccg cttcgttcag ttcgttacga ataagatcct
     2881 ggtacatgag gatatccttc agcataaatg taatagacaa aatgcagtgt accggatacc
     2941 gccaaaagcg agaagtacgg gcaggtgcta tgaccaggac tttttgccct gaagtgtaga
     3001 taaaaacagc aacaatgtga gcttcgttgt aattatattg taaacacatt gctaaatgtt
     3061 tttacatcca ctacaaccat atcatcacaa gtggtcagac ctcctacaag taaggggctt
     3121 ttcgttatga tgattttgag tattctcgct acagttgtcc tgctcggcgc gttgttctat
     3181 caccgcgtga gcttatttat cagcagtctg attttgctcg cctggacagc tgccctcggc
     3241 gtcgctggtc tgtggtcggc gtgggtactg gtgcctctgg ccattatcct cgtgccgttt
     3301 aactttgcac ctatgcgtaa gtcgatgatt tccgcgccgg tatttcgcgg tttccgtaag
     3361 gtgatgccgc cgatgtcgcg cactgagaaa gaagcgattg atgcgggtac cacctggtgg
     3421 gaaggcgact tgttccaggg caagccggac tggaaaaagc tgcataacta tccgcagccg
     3481 cgcctgaccg cagaagagca agcgtttctc gacggcccgg tagaagaagc ctgccggatg
     3541 gcgaatgatt tccagatcac ccatgaactg gcggatctgc cgccggagtt gtgggcgtat
     3601 ctgaaagagc atcgcttctt cgcgatgatc atcaaaaaag agtacggcgg cctggagttt
     3661 tcagcttatg cccagtctcg tgtgctgcaa aaactctccg gcgtcagcgg gatcctggcg
     3721 attaccgtcg gcgtgccgaa ctcattaggc cccggcgagc tgctgcaaca ttacggcact
     3781 gacgagcaga aagatcacta tctgccgcgt ctggcgcgtg gtcaggagat cccttgcttt
     3841 gcactgacca gcccggaagc gggttccgat gcgggcgcga ttccggacac cgggattgtc
     3901 tgcatgggcg aatggcaggg ccagcaggtg ctggggatgc gtctgacctg gaacaaacgc
     3961 tacattacgc tggcaccgat tgcgactgtg cttgggctgg cgtttaaact ctccgacccg
     4021 gagaaattac tcggcggtgc agaagattta ggtattacct gtgcgctgat cccgaccacc
     4081 acgccgggcg tggaaattgg tcgtcgccac ttcccgctga atgtgccgtt ccagaacgga
     4141 ccgacgctcg gtaaagatgt cttcgtaccg atcgactaca ttatcggcgg gccgaaaatg
     4201 gccgggcaag gctggcggat gctggtggag tgcctctcgg taggccgcgg catcaccctg
     4261 ccttccaact caaccggcgg cgtgaaatcg gtagcgctgg caacgggcgc gtatgctcac
     4321 attcgccgtc agttcaaaat ctctatcggt aagatggaag ggattgaaga gccgctggcg
     4381 cgtattgccg ggaacgctta tgtaatggac gctgcggcat cgctgattac ctacggcatt
     4441 atgctcggcg aaaaacctgc cgtgctgtcg gctatcgtta agtatcactg tacccaccgc
     4501 gggcagcagt cgattattga tgcgatggat attaccggcg gtaaaggcat tatgctcggg
     4561 caaagcaact tcctggcgcg tgcttaccag ggcgcaccga ttgccatcac cgttgaaggg
     4621 gctaacattc tgacccgcag catgatgatc ttcggacaag gagcgattcg ttgccatccg
     4681 tacgtgctgg aagagatgga agcggcgaag aacaatgacg tcaacgtgtt cgataaactg
     4741 ttgttcaaac atatcggtca cgtcggtagc aacaaagttc gcagcttctg gctgggcctg
     4801 acgcgcggtt taaccagcag cacgccaacc ggcgatgcca ctaaacgcta ctatcagcac
     4861 ctgaaccgcc tgagcgccaa cctcgccctg ctttctgatg tctcgatggc agtgctgggc
     4921 ggcagcctga aacgtcgtga gcgtatctcg gcacgtctgg gtgatatttt aagccagctt
     4981 tacctcgcct ctgccgttct gaagcgttat gacgacgaag gccgtaatga agccgatctg
     5041 ccgctggtgc actggggcgt acaggatgcg ctgtatcagg cagaacaggc gatggacgat
     5101 ttactgcaaa acttcccgaa ccgcgtggtt gccgggctgc tgaatgtggt gatcttcccg
     5161 accggacgtc attatctggc accgtctgac aagctggatc ataaagtggc gaagatttta
     5221 caagtgccga acgccactcg ctcacgtatt ggtcgcggtc agtacctgac gccaagcgag
     5281 cataacccgg ttggtttact ggaagaggcg ttggtggatg tgattgccgc cgacccgatt
     5341 catcagcgga tctgtaaaga gctcggtaaa aatctgccat ttacccgtct ggatgaactg
     5401 gcgcacaacg cgctggcgaa ggggctgatt gataaagatg aagccgctat tctggtgaaa
     5461 gcggaagaaa gccgtctgcg cagtattaac gttgatgact ttgatccgga agagctggcg
     5521 acgaagccgg taaagttgcc ggagaaagtg cggaaagttg aagccgcgta attgaataac
     5581 ggagccgaaa ggctccgttt ctttatccgc taattattta aaattaaagc catccggatg
     5641 gttttccagg ctgccggtca acgccgcgaa cagcaccgtt ttaccatcaa tcgaaagcgc
     5701 atcgttcaca ttcaaccagg tcagtttctc ttgcgacgtt ttctcatcaa tagtcgagaa
     5761 cagccccgtc atctgattag atttctcgga ccacatcaca gcgatacgtt gcgagccaca
     5821 gtcatgcggt ttgcacgcgc tcatcacctg atacgtctca tctcccaacg ttacggtttg
     5881 tgcaggggta taagtaccgc ctttcatcac ccaggcaggc agcttatgcc cttgcaccat
     5941 ctgattaaat gcagctttgg tggtttcgcc ctttgcaagg ctgctaatgg ttaaatcatc
     6001 ctgcgccatt gcactggtgg cgatgaccag tgcggcgact gtcgttattg ccttaaacat
     6061 cattcctccc gagcttatcc tgcccatgtt attaacctcc taaagcctgg cgataacacg
     6121 ataatttgca aatcagatat ttccaatatc agcatttgtt atcaatttat tctgcgctaa
     6181 ggcaactgcc tgataaggtg ttaaagttat aaaacccttc gcataaagga gttgtacacc
     6241 gtgcctggtt tgaagattac gcttttgcag caaccactgg tgtggatgga tggtcctgcc
     6301 aacctgcgtc attttgatcg tcaactggaa ggtattaccg ggcgcgatgt gatcgttcta
     6361 ccggagatgt ttaccagcgg ctttgccatg gaagcggctg cttcgtcgct agcacaagat
     6421 gacgtagtga actggatgac agccaaagcg cagcagtgca atgcgctgat tgcaggcagt
     6481 gttgcattac aaacggagtc tggttcggtt aaccgctttt tgctggttga gccgggcggc
     6541 acggtacatt tttatgataa gcgtcatctg ttccgcatgg cagatgagca tctgcattat
     6601 aaagcgggca atgcgcgagt gattgtggaa tggcgcggct ggcgtatttt gccgttggtg
     6661 tgctacgact tacgttttcc ggtgtggtcg cgcaatctca acgattatga cctcgccatc
     6721 tacgttgcca actggcctgc tccgcgctct ctgcactggc aggcattgct gacggcccgc
     6781 gcgattgaga atcaggcgta tgtggcggga tgcaatcgcg tcggcagcga tggcaacggc
     6841 tgccattatc gcggtgacag ccgggtgatt aatccacaag gcgagattat cgctactgcc
     6901 gacgcgcatc aggcaacgcg cattgatgcg gagctgtcga tggtggcatt gcgggaatat
     6961 agagaaaagt ttccggcatg gcgggatgcg gatgagttta ggttgcggtg agtgggggct
     7021 gtatttaggg cggggaattc agaaagggat gtttagtgtg ctgagcgaga gttaaataat
     7081 aaaaaactgg gaagatttaa taatcccccc gcagggcaag agcatgaatt aaatctgtgt
     7141 taaatttttc attttgtcag ttcgaaaccc aagctctgag caggatgcaa gcacttcttc
     7201 tatataacat gtgtttaacg caaccttctt tcttttcctc ttcaccccac ccttaatgtc
     7261 tttgcaatct cttaataaat tcagtgccat cttctttatt ccagatatta tttcggctgc
     7321 gtttcctctt cttatccggc tggcatcttc attcattttt acatctaaca cccaatgaag
     7381 a
//
LOCUS       DBFIFI010000208         7332 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.208, whole genome shotgun sequence.
ACCESSION   DBFIFI010000208 DBFIFI010000000
VERSION     DBFIFI010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7332
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.208"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            183..419
                     /gene="lpp"
                     /locus_tag="N7B39_004061"
     CDS             183..419
                     /gene="lpp"
                     /locus_tag="N7B39_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HGU2423944.1"
                     /translation="MKATKLVLGAVILGSTLLAGCPSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(482..1486)
                     /gene="ldtE"
                     /locus_tag="N7B39_004062"
     CDS             complement(482..1486)
                     /gene="ldtE"
                     /locus_tag="N7B39_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HGU2423945.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVAVSSGATPTASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(1635..2051)
                     /gene="sufE"
                     /locus_tag="N7B39_004063"
     CDS             complement(1635..2051)
                     /gene="sufE"
                     /locus_tag="N7B39_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HGU2423946.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDVVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            complement(2064..3284)
                     /gene="sufS"
                     /locus_tag="N7B39_004064"
     CDS             complement(2064..3284)
                     /gene="sufS"
                     /locus_tag="N7B39_004064"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HGU2423947.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSAQGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            complement(3281..4552)
                     /gene="sufD"
                     /locus_tag="N7B39_004065"
     CDS             complement(3281..4552)
                     /gene="sufD"
                     /locus_tag="N7B39_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HGU2423948.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVPALSDATE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLELAEGAEATVIEHFVSLNDARHFTGARFSINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENCTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLTRIGQRLPGGAR"
     gene            complement(4527..5273)
                     /gene="sufC"
                     /locus_tag="N7B39_004066"
     CDS             complement(4527..5273)
                     /gene="sufC"
                     /locus_tag="N7B39_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HGU2423949.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            complement(5283..6770)
                     /gene="sufB"
                     /locus_tag="N7B39_004067"
     CDS             complement(5283..6770)
                     /gene="sufB"
                     /locus_tag="N7B39_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HGU2423950.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVVSDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            complement(6779..7147)
                     /gene="sufA"
                     /locus_tag="N7B39_004068"
     CDS             complement(6779..7147)
                     /gene="sufA"
                     /locus_tag="N7B39_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HGU2423951.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAVHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
BASE COUNT         1700 a         2011 c         1771 g         1850 t
ORIGIN      
        1 atccgatgga agcatcctgt tttctctcaa tttttttatc taaaacccag cgttcgatgc
       61 ttctttgagc gaacgatcaa aaataagtgc cttcccatca aaaaaatatt ctcaacataa
      121 aaaactttgt gtaatacttg taacgctaca tggagattaa ctcaatctag agggtattaa
      181 taatgaaagc tactaaactg gtactgggcg cggtaatcct gggttctact ctgctggcag
      241 gttgccccag caacgctaaa atcgatcagc tgtcttctga cgttcagact ctgaacgcta
      301 aagttgacca gctgagcaac gacgtgaacg caatgcgttc cgacgttcag gctgctaaag
      361 atgacgcagc tcgtgctaac cagcgtctgg acaacatggc tactaaatac cgcaagtaat
      421 agtacctgtg aagtgaaaaa tggcgcacat tgtgcgccat ttttttgtct gctatttacc
      481 actactgcgt cgcgcgtaac atattccctt gctctggttc accattctgc gctgactcta
      541 ctgaaggcgc attgctggct gtgggagttg ctccactgct caccgcaacc ggataccctg
      601 cccgacgata caacgcttta tcgactaact tctgatctac agccttattg tctttaaatt
      661 gcgtaaagcc tgctggcagt gtgtatggca ttgtctgaac gttctgctgt tcttctgccg
      721 atagtggtcg atgtacttca acataacgca tcccgttagg ctccacggaa tatttcactg
      781 gttcgttgat cactttcacc ggcgttcccg tccgcacgct ggagaacaag gctttaatat
      841 ccggtgcatt catgcgaata caccctgaac tgacgcgcaa accgacgctg tccggcgcac
      901 tggtgccatg aatgaggtat tcgccattac catgcgcgag gcgcagtgcg taacgtccta
      961 gcgggttatt tggtccggca ggaacgactg gcggtaattt aatgccacgc tccagcgaac
     1021 gttgacgaat gcctgccgta ggcgtccagg ttgggttagg gattttctgc cccacacgcg
     1081 tttccatcac tggcgtttcc agcccctgca agccaatacc tatcggataa acctgcacga
     1141 tattttctcc cggcggataa taataaaggc gcagctctgc aaggttaacg ataatcccct
     1201 gacgcggcgc atcaggtaat aacagttgcg aaggaatagt tatcgtcgta ccgggttttg
     1261 gcaccggggc gatagtgtta ttggcttcaa ggatcaacat tgccgcagta tcaaaacgtc
     1321 gggcgatggc ctgaaggttt ttatcccctt cctgcaccgt atacgtttga ttttgcccaa
     1381 ccagtcggct tccggttggt ggtagcggat aatcaaccgc ccaggcagcc tggatggcac
     1441 taaaagcgcc gataagcgtg agtgtaagca aagacgcgcg tttcattgta aacctcctgt
     1501 atttgccgga gactcacgct gaaacgtcgg atggcgctta tgttcacctg aaaccgaaac
     1561 actcctgtgc aggtcagtgt aaacattgac catccggcaa tgtgagccaa ccggatgaaa
     1621 gctgtccctt tagtttagct aagtgcagcg gctttggcgc gaattgcgcg aatcatcgct
     1681 tccagacctt gtgaacgaga tggggtgaga tgttgggtga gtgccatttt ttcaaaccac
     1741 ggacgcacat cgaaattgac aacatcctgc ggcgtcatct gatcgtagag aataaagacg
     1801 accgcaataa gccctttcac aatcgccgca tcgctgtcgc cctgcaattc aataattccc
     1861 tgcgcattct gacgcatgac aatccacacc tgactctggc agccctgaat gctattttgt
     1921 gggcttctgt cttcgtcgcg taattctggc agacgctggc ccagctcaat aatgtagaga
     1981 tatttctctt cccagttggc gcagcgtaaa aaattacgca gcaacttttc tttatccggc
     2041 aataaagcca tagtgcctcc ctgttatccc agcagacggt gaatacgttg cagtccggtc
     2101 accagacgat ccacttcttc atgggtgtta tacatggcca gcgacgcccg acacatagca
     2161 gggacgttat aataggccat taatggcata gcgcagtgat gtccggtacg cacagcaatg
     2221 ccgtaattat cgagaaaact gccaacatca taggcgtggt gtttaccgag attaaaagca
     2281 ataacgccaa gcctgttttg tgggccatag agagtgagat ccggtacaga ttccagctgt
     2341 gatagcgcgt aatgcatcag attctgttca tactcggcta tgttattaag cccctgcgcc
     2401 gaaacatact ccagcgccgc gccaagacca atgatgcccc cggtattggg tgtaccggct
     2461 tcaaaccgcc acggtgcttt ggtccaggta gtgccttcac tcaggctgac ggtggcgatc
     2521 atagaaccgc ccccttccca cggcggcatc tcctgtagca aggcttcttt gacataaaga
     2581 atgccaatcc cggtggggcc atacagttta tgcccggaaa acacgtaaaa atcgcaatcc
     2641 agcgcctgaa catccaccgg atgatgcatc accgcctgag cgccatccac cagcactttt
     2701 gcgccatgct ggtgcgcaag cgtgatcatt tccgccagtg gattttctgt gccaagcacg
     2761 ttggagacat gagtaattgc cagcaggcga gttttctcat caaacagcgt aggcagcgtc
     2821 tccagttgca acgtaccgtc gggattgagc gggatcacac gcagctctgc gccaacgcgt
     2881 gcgcaaagca tctgccaggg aacaatgtta gcgtggtgct ccatctgact tatgatgatg
     2941 ttatcgcccg cccgcacgtt gctgttgccc cagctattgg cgaccagatt gatcccttcc
     3001 gtcgtgccgc ggacgaacac cagctcttcc gccgaacggg cattaataaa cagcgatgcc
     3061 cgcttgcgca cgttctccat tttctcggtc gcctgggcgc ttaaggtatg aataccgcga
     3121 tgcaccgccg cgtagccatg acgataaaac tcggcctcgg cgtcaatcac ctggctcggt
     3181 ttctgcgcac tggcggcgct gtcgagataa gccagcggca aaccgtttac ctcacgcgaa
     3241 agcaccggaa agtcggcccg cactttgtcg acggaaaaag tcatcttgca cctcctggca
     3301 gccgttgacc gattcgggtc agcacctgct gtttaagccc ctcatcacgc agtgcttccg
     3361 tcagttcggc agcaaaggcg taaatgatca tctgctgggc atcctgctga ttgatcccgc
     3421 gcgagcgcag atagaacatc tgttcatcat caatacgccc caccgtcgcg ccgtggctgc
     3481 atttcacatc atctgcatag atttccagct gcggtttcgt atccacttcc gccagtttgc
     3541 ccatcagcag attattgttg gtcatctgac catccgtttt gatggcgtgc tgcgcgacgt
     3601 tgatcaaacc gttaaatacc gcgcggcctt tgtcgctgac gatagttttg tgcaactgtc
     3661 ggctgttaca aaaaccttta ttgtgttcca gccaggtacg ggtatcacac acctcgtttt
     3721 tcaccggcat cgccaggcta ttgatccgca gcgtgcagtt ttcgccattg agttgcgtac
     3781 tggtgttgtg tcgtaacact gcgccaccca gcaggaaact gtggctaaat gcggtggcat
     3841 cgtcagccag caacaaatcg ttatgggcaa agtggtgact gagcgggttt tcaaacgcca
     3901 gcttgatatg ctgcaagtgg gcattcgctg cgacgttgat agagaaccgt gccccggtaa
     3961 aatgacgagc atcattaagg ctgacaaaat gttcgatcac cgttgcttcg gcaccttccg
     4021 ccagctccag atgatgtcgg taatgggcag tgttcacctc ttcacctgcc acgccctggg
     4081 tgatatgcat taacagcaat ggctttgccg gccgttgacc gcgcttcacg gcgatatgcg
     4141 tcacgctttg tgccaggctt tccgtcaaat gcagaaacac ttccgcctga atagcatcgg
     4201 gtaacccctg acggtcgtcg ttaatgctca cttcataccc gctgccttca gtcgcatcgc
     4261 tcagcgcagg cacgtaacgt ccatcgacaa ataccagccg cacagcgtct aacgttaacg
     4321 ctaaggcatc tcgctgctgt ggggatatct ctcccgcaat gctgacaaac tgactattgg
     4381 tcagcccttc cagcggcgta tatttccagt tttcatgttt acgtgtcggc agtccggtac
     4441 gcagcaattg ttgtaaatgc tgctgtgctt gcggggagcg ttttgtccct tcagcttcaa
     4501 acaagtgatg ccactgttgc agcgcgttac tgctgttcgg taagccagcc ataaccctgc
     4561 tcctccagtt gtttgaccaa cgtgaaatcg ccggatttca caattcgtcc ctgatatagc
     4621 acatgaacgt aatcaggctt gatgtagtcg agaatgcgtt ggtagtgcgt aacaatgatg
     4681 aatgagcgct tgccatcacg cagcgagttc acgccatcgg cgaccacttt taatgcgtcg
     4741 atatccagcc cggagtccga ttcatcaaga atgcataact ccggttccag caccgccatt
     4801 tgcaaaatat cgttgcgctt tttctcgccg ccggaaaaac caacgtttac cgaacgggtt
     4861 aataaatctt ccggcatctt caggagagcg attttctctt ccatcaaatc ctgaaaatca
     4921 aagcggtcga gcgtttcctg gccgcgatag ctgcgcaccg cattaagtgc cgtttgcagg
     4981 aaaaactggt tactgacacc tggaatctcc accggatact ggaaggccat aaagatgcct
     5041 tcgcctgcac gatcttccgg cgacagtgca agcaaatctt tgcctttgaa ctcaaccgtg
     5101 ccgcccgtca cttcataatc ttctcgcccg gcaagcgttg ccgataaggt acttttgccc
     5161 gaaccgtttg gccccataat ggcgtgaact tcgccgggac gaacgtcgag gcttaatcca
     5221 cgcaggatag ctttatcttc cacgctgacg tgtaaatctt taatacttaa catgtttatt
     5281 ccttatccga cgctgtgttc aagactgatg gcgaggagtt tttgtgcttc aacggcaaat
     5341 tccagcggca gttctgagaa cacgtctttg cagaaaccgt taacaatcat cgagatggcg
     5401 tcttcttcgc tgatcccgcg ttgcaggcag taaaacagtt gatcttcacc aatacgtgat
     5461 gtcgttgcct cgtgctccag ttgtgcgctg ttattgcgac attcgacata cgggaaggta
     5521 tgcgccccac aattagcgcc aatcagcatt gagtcgcact gagtgaaatt gcgcgcattg
     5581 gttgccgtcg gcatgatttt cactaagccg cgataactgt tctggctatg tccggcagag
     5641 atccctttcg agataatggt cgatttggtg tttttaccga tgtggatcat cttggtgccg
     5701 gtatccgctt gctgatgacc gctggtcagc gccactgagt aaaactcacc aatggagtta
     5761 tcgccgcgca aaatgcagct gggatatttc cacgtaatcg ctgacccggt ttctgattgc
     5821 gtccatgaca ttttgctgtt ttcgccttcg cacaaagcac gcttggtgac gaagttgaga
     5881 ataccgccgg tgttgttatc gccagggaac cagttttgta ccgtggaata tttcacctcg
     5941 gcgtttttat ggatgatgac ttccaccact gccgcgtgca actgatagct gtcacgcacc
     6001 ggagcggaac agccttcaat gtagctgacg tagctgtctt cgtcggccac cagaatggtg
     6061 cgctcaaact gtccggtttt ttccgcgtta atgcgaaaat aggtggaaag ttccatcggg
     6121 cagcgcacgc ctttaggcac ataaataaac gtaccgtcag agactaccgc cgcattaagc
     6181 gcggcaaaga agttgtcatt ccccggcacc acggtgccga gatatttacg caccagttcc
     6241 gggtgatcgt ggatcgcctc accaaaggaa cagaaaataa ttccctgctc cgccagtttt
     6301 tcgcgataag tagtggcaac cgaaactgag tcgaaaatgg catccaccgc cacctctttg
     6361 ccttcccgca cgggaacacc caactgctca aacgccgcct ccacctcttt acttaaaaag
     6421 gcgttcgcgc cagtttgctg caccgcgcca ggttcagacg cgcaagtgtc gtcacaatta
     6481 ccgcacgatg gtgccgagta gtagctgtaa tcctgataat tcagcttgtc gtagtgcgct
     6541 ttcaaccagt gcggttcttc catctccagc catgcgcgat aggcgtttag acgaaactcc
     6601 agcatccact ccggctcatt acgcttcgcc gaaattgcgc gcaccacctc ttcgtttatc
     6661 ccctttgcca gctcatcggt ggctaactgg gtgaagaatc cttctttata attcagcggg
     6721 ccgccggtcc aggttttgac atcgtcagtt gcttcagtat tacgagacat agtaccgcct
     6781 ataccccaaa gctttcgcca cagccacatt cattctgggc tttagggttg tgaaatttga
     6841 atatctgatt aagtccttca cgaacgaaat cgacttccgt gccatcaata aacggcatcg
     6901 cttgcagcgg gacaaacagc ttcgcgccgt cgtgttcaaa cagcagatcg tctttgtccg
     6961 gctcgctaac actgtcgagc acatagccaa agcccgcgca gcccgtttgc ttcacaccta
     7021 agcgcacgcc gaccataccc ggctgctttg ccaccagctc gcggatgtgg acagcggctg
     7081 cgggtgtcag cgttaagcct tgccaggcga aatcctgtgg attaaaggtt cctgaatgca
     7141 tgtccatcga tttacctcac ttcatcgctt tcagcgtata acagcatgtt agtgataatg
     7201 attatcagtt caacccagca aaggcagggg ctttaccgta aaacatgctt tttgcctgct
     7261 tttaataagc atagaccctg atgtgtgggt taacaggcac gctaagaatt aggtatctca
     7321 ttgttagata at
//
LOCUS       DBFIFI010000209         7310 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.209, whole genome shotgun sequence.
ACCESSION   DBFIFI010000209 DBFIFI010000000
VERSION     DBFIFI010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7310
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.209"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(193..828)
                     /gene="msrQ"
                     /locus_tag="N7B39_004069"
     CDS             complement(193..828)
                     /gene="msrQ"
                     /locus_tag="N7B39_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HGU2423952.1"
                     /translation="MRLTAKQVTWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWIILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKSRSLFNRLRKQVHNKLSV"
     gene            complement(829..1833)
                     /gene="msrP"
                     /locus_tag="N7B39_004070"
     CDS             complement(829..1833)
                     /gene="msrP"
                     /locus_tag="N7B39_004070"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HGU2423953.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKALEFSKPAAWQNNLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            complement(1941..2354)
                     /gene="hiuH"
                     /locus_tag="N7B39_004071"
     CDS             complement(1941..2354)
                     /gene="hiuH"
                     /locus_tag="N7B39_004071"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HGU2423954.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            2487..3158
                     /gene="hprR"
                     /locus_tag="N7B39_004072"
     CDS             2487..3158
                     /gene="hprR"
                     /locus_tag="N7B39_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HGU2423955.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3158..4516
                     /gene="hprS"
                     /locus_tag="N7B39_004073"
     CDS             3158..4516
                     /gene="hprS"
                     /locus_tag="N7B39_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HGU2423956.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNSYLNIDIASPGAK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRITLPQ
                     RN"
     gene            complement(4624..5475)
                     /gene="hchA"
                     /locus_tag="N7B39_004074"
     CDS             complement(4624..5475)
                     /gene="hchA"
                     /locus_tag="N7B39_004074"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HGU2423957.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPQKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKLLTGDSPFAANALGKLAAQE
                     MLAAYAG"
     gene            complement(6067..7253)
                     /gene="ompC"
                     /locus_tag="N7B39_004075"
                     /pseudo
     CDS             complement(6067..7253)
                     /gene="ompC"
                     /locus_tag="N7B39_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
BASE COUNT         1990 a         1565 c         1734 g         2021 t
ORIGIN      
        1 caacaactct taggagatga gagcatagtt ttctcaggtg tacttatcta tcttattgaa
       61 ataaaatgaa ttatgttgat aatttgaggt tgatgacaaa cgatacataa cctgatgtga
      121 tctgcgtagg ccggataggg cgctcgcgcc acatccggca gggataacgc gtactttgtc
      181 ggcaatatga agttatacag ataatttgtt gtgaacctgc ttgcgtaacc ggttaaaaag
      241 ggaacgcgac ttcttatacc gtaaggctaa aagcagtaca gccagcccag cgtagatgag
      301 cggctgcggt gaaataatct tcactgacca cagataatgt attggggcca ggatcgcgac
      361 aagatagacg aaattatgca actgttgcca gtgcttgccg agttttcgct gcatcgcctg
      421 ggttgaagta aacgctaaag caagcaaaat aatccagctg ataattccta acgttaaata
      481 aggccgggta attaactcct tgcctaataa cgccagatta ttcacgccca gctccagcaa
      541 tgcgtaactg gttaaatgca gtgtcgccca ggcaaagcac cataagccta acaggcggcg
      601 tgtgcgtatc aataacggct gttttgcgta gcgtgccaga ggtgtgatta acaaggtcgc
      661 cagcaaaaat ttcagtgcag tgcgaccggt aaaatgctga atatctttca ccggatcggc
      721 acccagtcca ccgtgattga tcgcccagac cagccagaga aacggcaaca atccggcaag
      781 atgcaggcaa actttcagcc atgtcacctg ttttgctgtc agacgcattc agaaattctc
      841 ccgcaaatcc aggccacgat acagcgatgc cacctggtcg gcgtaaccat taaacagtag
      901 cgttggctgg cgctgtacat cgaggatgcc gcctgaacca ataaatcgtt cggtagcctg
      961 tgaccagcgt gggtgatcaa catgcggatt aacgttggcg taaaaaccgt attcgtcagg
     1021 cgctgccaga ttccaggtgg ttggcggacg ctcgcgggtc agcttaatac tgacgatcga
     1081 tttaatccct ttaaagccat atttccacgg cacaatcagt cgcaccggcg cgccattttg
     1141 tggcggtaac gccttgccat aaacacctac ggtcatcagt gtgagcggat gcattgcttc
     1201 gtcgagacgc aatccttcga cataaggata tttcagcccg ccgccgataa agcggtcctg
     1261 ctggcctggc atctgttccg gtgcataaat tgtttcgaaa gcgacatact tcgcattgct
     1321 ggtgggttcg gcaagcgcca gcaatttgtg cagcggaaaa ccaatccacg gcaccaccat
     1381 cgaccacgct tccacgcagc gcatacgata aatacgctct tccagcggga agcgacgggt
     1441 taaatcatcg tgatcgaggg tcaatggttt tgccacttcg ccgctgattt tcagtgtcca
     1501 tggatcggtt ttcaggctac cagcattagc ggcgggatcg gctttatcca gcccgaattc
     1561 atagaagttg ttataaccgg agactttatc tgctggcgtc agtggcaggt tattttgcca
     1621 ggcggcaggc ttgctgaact ccagcgcttt tccggcgggg gcgggtgggc gatcgttccc
     1681 tttaaaccag ctaagcagat cggcatgcgc agcgtgaggc aaagaaagtg cagctgcgct
     1741 gatgcccagt gcttttaaca cctgccgacg cttcataaag aataccgact cggccgtaac
     1801 atctgattct tttaaaaatt gattcttttt cataagccat cacacttttt gtcgttagcc
     1861 agcaagagcc gtttactgtt aatctgtcag ggagtgtcct ccctaacagt agcagagatc
     1921 tgcttttaca tatttatgca ttaactgcca cgataggttg aatacccata ttggctaagt
     1981 aataaaggca catgataatg ctcgttcact ttattaatat gaaattcaac ggggatctca
     2041 gggaagaaac tttcaagatt ttgtttcttg aaatagtccc cggttttaaa tacgacacgg
     2101 taatcgcccg tagttgcagt ttgctcgggc cacagtgcct taattcgtcc atccttatct
     2161 gttttggcgg tattaagttg taaccagccg ttgtccgctt tcttttcaag agtgactgtc
     2221 acgtcggcag cgggttttcc tgtttgctgg ttcaaaatgt gcacgctaag aatgttttgt
     2281 tgtgcagcat agaccaaaga aggtaatgaa aatgcagccg ttactacgga gagtactaaa
     2341 taacgcttta acatgtttat atccttgtca tgtgaatgag ttttgaggat agcgaaaaag
     2401 cgtccagggt aaccttacag cagcattaca attttgtaat gaaccaggct gtttctatga
     2461 catatgattt atggcatatt attttcatga agattttact tattgaagat aatcaaagga
     2521 cccaggagtg ggtaacgcag gggctttccg aagcgggtta tgtcattgat gccgtttctg
     2581 atggcagaga tgggctttat cttgcgctga aggatgatta tgcattgatc attctggata
     2641 ttatgcttcc gggtatggat ggctggcaga tcttacaaac gttaagaacg gcaaagcaaa
     2701 cccctgttat ttgccttact gcaagggatt ctgtcgatga cagagtcaga gggctggaca
     2761 gtggagcaaa tgattatctg gtaaaacctt tttcattttc tgagttgctg gcaagggttc
     2821 gggcacaatt aaggcaacat cacgctttga attcaacatt agaaatcagc ggcttaagaa
     2881 tggactctgt tagtcaaagt gtgagcaggg acaatatcag tattacactg acgcgcaagg
     2941 agtttcagtt actttggcta ctggcctcca gagctggcga aattataccc agaacggtta
     3001 ttgcgagtga aatttgggga atcaactttg atagtgatac caatacggtg gatgtcgcca
     3061 ttcgcaggct ccgcgcaaaa gttgatgatc cttttcctga aaagctaatt gccacaatcc
     3121 gtgggatggg atattcgttc gtagcggtaa aaaaataatg aaaagattat ctataaccgt
     3181 ccgtttaacc ttgcttttta tattgctgct gtctgttgct ggcgccggaa ttgtctggac
     3241 tctctataat ggcctcgcaa gtgagttgaa atggcgcgat gatacaacac tcattaaccg
     3301 gacagcgcag atcaagcagt tattaattga tggggtaaat ccagatacgt tacctgtata
     3361 ctttaaccgg atgatggatg ttagtcagga tatcttgatc attcatggtg atggcatcaa
     3421 taaaattgtt aaccggacaa atgtcagtga tgacatgtta aataacatac ctgctagtga
     3481 gacaatcagc gcagctggca tttacagaag cattattaat gatacagaga tagatgcttt
     3541 acgaattaat attgatgaag tttcgccatc attaacggtt actgtggcta aattggcttc
     3601 agccagacat aacatgcttg aacagtataa aatcaatagc attataattt gcattgtcgc
     3661 cattatactg tgctcagtat taagtccgct gttaatccga acggggttac gagagatcaa
     3721 aaagttgagt ggtgtaacgg aagcgctgaa ttataacgat agccgggagc ctgttgaggt
     3781 tagcgcatta ccgagagaac taaaacctct tgggcaggcg ttgaataaaa tgcatcatgc
     3841 tttagtcaaa gattttgagc gtctaagtca gtttgctgac gatctcgctc atgaacttag
     3901 aacgccaatt aatgcattac tgggtcagaa tcaggttacg ctcagtcaaa ccagaagtat
     3961 cgctgaatat caaaaaacaa ttgccggtaa cattgaagag ctggaaaata tttcgcggtt
     4021 aacagagaac atactgtttc ttgccagggc agataaaaac aatgttttgg tgaaactgga
     4081 ctcgctttct ctcaataagg aagtcgaaaa tttgttggac tatcttgaat atctttcaga
     4141 cgagaaagag atttgcttta aggtcgagtg caatcagcaa atctttgcgg ataaaatttt
     4201 actgcaacga atgttatcga atcttattgt taacgctatt cgatattcgc cagaaaaatc
     4261 gcgtattcat ataaccagtt ttcttgatac caacagctat cttaatattg atatcgccag
     4321 ccctggagcg aaaattaatg agcctgaaaa actcttccgt agattttggc ggggagataa
     4381 ttcgcgtcat tccgtaggtc agggacttgg tctttcttta gtcaaagcga ttgccgaatt
     4441 gcatggggga agtgctacgt atcactatct caataagcat aatgtgttcc ggattacgtt
     4501 gccgcaaaga aattaacata gggcttcagt acgccataaa ttaaccgtga aaaaacgcag
     4561 ccacgtcgtg agtactaacg cggctgcgat tgattatgcg cttacattca aacgtaacag
     4621 ggattaaccc gcgtaagctg ccagcatttc ctgcgccgcc agtttaccca acgcattcgc
     4681 tgcaaaagga ctgtcgccgg tgagaagttt acggtcctta tgtactcgcc cggtgatgtc
     4741 gtcattaatg atgttcatgc ccattttctt cagttcttcg ccgaagtacc aggtgagatg
     4801 acccggcata tagccaatct ctggcgtttg tttgtctgcg gcgtctggga atgcgcaaat
     4861 ggaataacca ttcagtggat tatcgccgtg gcgaagcgcc agaaaagccg ccgggccgtg
     4921 gcaaagggag ataacaaaac ggtcattttt gattgcccac tgtaaagcgg cagctacgtc
     4981 ctggctttca ggtaaaccaa taagtgcgcc atgaccacca ggaacaaaga ttgctgcata
     5041 ttcgctatca gcgttgaggc tggcgacaac atccgcgagt ttcttcggat tgcggaacaa
     5101 cgatttatgc tgctcaaaga atggcattac tttttcatct ttctgcggca tagcccagta
     5161 ttcaaacttg gtcatcagac cggaaatggt cgccacttcg aattcgaaac ctgcagcatg
     5221 gagatgatac aacggcagca acgtttcaat cggatggtta ccggtcgaga acagttttcc
     5281 gttatcggtc ggcaaataac gttcgtccgc ggcgatcacc agaattttat gtttaccgcg
     5341 ataaggtttt ggatagtcca cgccatcaag atcagagaca ggactggtat attggctaag
     5401 cgaatattct gaagggaaga atgcattatc ttcagcaata tcgacctgcg gatttttact
     5461 ggtttgaaca gtcatagtgt attccttatt gttgcttagt tagggtagtc actatatttg
     5521 cgactgagct ggtcgctatt tttaaccagg ctggaaaatt gagtcaatat atcgaaagcg
     5581 gtagtcggcg ggtgtaagta tgtaatgatg atgctgcact ggtgttgaat attattcacg
     5641 ccaaattttc cttattgagc aacttcgtcg caagtggcct caatgatttt agaccattat
     5701 cagaggtacg gggcggcggg gagagattta gtgctgtgga atattgtggt gctgatgaat
     5761 atttatatcg cggattggta gagaaaaagc ttatttcatg cactattttt gcaaatattt
     5821 ctatatggga agcgtgtttg ttacggatta attcataaag aaatgcattt gggtgttggc
     5881 ttgaagagtc gctggttgat gttattgtgc caggaggggc agggtaaggg ggtgtatcga
     5941 taagctttgg caattgcgtg tgccgcagat ggagatgaaa tgccaataaa aaagcctgag
     6001 ttcgatgagg gacgaactca ggctagtgtt cagttttaca ataacacgcc gcggcgcgtt
     6061 ggctgattag aactggtaga ctaagcccac agccacgata tcatcggtag cgatgccgtt
     6121 ttctttgtag aagtcgtcgt cgttgtccag caggttgatt ttgtaatcaa cataagtgga
     6181 catgtttttg ttgaagtagt aggtcatacc aatatcaaca tatttaacca gatctttgtt
     6241 ggtgtaatga tgattaccct ggctgtcgaa gttatcacta tttacaccgc ctaaatcctt
     6301 acctttagat tgcagataag cgatggacgg gcgcagaccg aagtcgaact gatactgagc
     6361 aacaacttca aagttttggg ttttgttagc gatggcatca gcatcaccgt aaccagtcat
     6421 gttacggggt tcagcataca tagctgctaa gtaaacgtta ttagcgtcgt atttagcacc
     6481 aacggtccat gcttctgctg tttcaccgcc agcagttgca tcgaagcgat ctccataacg
     6541 agtgttatga gtacctttgt gaacctgatt gtcggtacgg tcagagttag agtaagcagc
     6601 gcccagactt aaaccaaagt caaagtcata ggaagtggac atactgaacc gtcgccgttc
     6661 tctttagcaa gtttacgtcc attaccgtta cctgaacctt cttgatcaaa taatccgctt
     6721 ttctcgttgt taccctgata ctggagtgca aagttcagac catcaacttg accaaagaaa
     6781 ccattgttac gataggttgc tacgctgtta gcacgaccgt tcatgaagtt gtcagcgcca
     6841 gcataggtat ctccaccaaa ttctggcagc atatcggtcc atgcttctac gtcatatacg
     6901 acaccgtagt tacgaccata gtcgaaagta ccattctgac caaagccgaa acctgcaaat
     6961 gccagacgcg ttgcggtatt acccttgtcg ctttcaggac cgtttgcgcc aatgttaaat
     7021 tcccactgac cataaccagt gaattggtca gcgatctgag tttcaccttt gaaaccgata
     7081 cgggcatatg acatgtcgcc atcgctgcta gcatcatcag agaagtagtg caggcccgct
     7141 acttttccgt acaaatccag tttattgcca tctttattat aaatttcagc cgcatttgct
     7201 gcgccagcaa ctaataacgc cgggaccagc attgccagaa cttttctttt cattatgtat
     7261 tcccttgtga ttataatctt catgaatata tcaataagtg ccgttatcca
//
LOCUS       DBFIFI010000210         7277 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.210, whole genome shotgun sequence.
ACCESSION   DBFIFI010000210 DBFIFI010000000
VERSION     DBFIFI010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.210"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..751
                     /locus_tag="N7B39_004076"
     CDS             <1..751
                     /locus_tag="N7B39_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HGU2423958.1"
                     /translation="TDAGAGFEQTLTGMTVGIDSRNDIPEGIATLGAFMGYSHSHIGF
                     DRGGHGSVDSYSLGGYASWEHESGFYLDGVVKLNRFESNVAGKMSSGGAANGSYHSNG
                     LGGHIETGMRFTDGNWNLTPYASLTGFTADNPEYHLSNGMESKSVDTRSIYRELGATL
                     SYNMRLGNGMEVEPWLKAAVRKEFVDDNRVKVNSDGNFVNDLSGRRGIYQAGIKASFS
                     STLSGHLGVGYSNGAGMESPWNAVAGVNWSF"
     gene            complement(1123..1389)
                     /gene="minE"
                     /locus_tag="N7B39_004077"
     CDS             complement(1123..1389)
                     /gene="minE"
                     /locus_tag="N7B39_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HGU2423959.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(1393..2205)
                     /gene="minD"
                     /locus_tag="N7B39_004078"
     CDS             complement(1393..2205)
                     /gene="minD"
                     /locus_tag="N7B39_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HGU2423960.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(2229..2924)
                     /gene="minC"
                     /locus_tag="N7B39_004079"
     CDS             complement(2229..2924)
                     /gene="minC"
                     /locus_tag="N7B39_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HGU2423961.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            3444..3812
                     /gene="ycgJ"
                     /locus_tag="N7B39_004080"
     CDS             3444..3812
                     /gene="ycgJ"
                     /locus_tag="N7B39_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HGU2423962.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLSEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(3915..4316)
                     /gene="pliG"
                     /locus_tag="N7B39_004081"
     CDS             complement(3915..4316)
                     /gene="pliG"
                     /locus_tag="N7B39_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HGU2423963.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            4387..4545
                     /locus_tag="N7B39_004082"
     CDS             4387..4545
                     /locus_tag="N7B39_004082"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2423964.1"
                     /translation="MAFLRSVKSRRGIFAGGITYKGAWTQQTVEIYSWLACGAGFIDV
                     SLCQNLAS"
     gene            4557..4850
                     /locus_tag="N7B39_004083"
     CDS             4557..4850
                     /locus_tag="N7B39_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HGU2423965.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKQALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            4922..5581
                     /gene="ycgM"
                     /locus_tag="N7B39_004084"
     CDS             4922..5581
                     /gene="ycgM"
                     /locus_tag="N7B39_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HGU2423966.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            5673..6119
                     /gene="ycgN"
                     /locus_tag="N7B39_004085"
     CDS             5673..6119
                     /gene="ycgN"
                     /locus_tag="N7B39_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HGU2423967.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(6326..7237)
                     /gene="hlyE"
                     /locus_tag="N7B39_004086"
     CDS             complement(6326..7237)
                     /gene="hlyE"
                     /locus_tag="N7B39_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HGU2423968.1"
                     /translation="MTEIVADKTVEVVKNAIETADGALDLYNKYLDQVIPWQTFDETI
                     KELSRFKQEYSQAASVLVGNIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLFD
                     EYNEKKASAQKDILIKVLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFS
                     EKSSYFQSQVDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSV
                     QSFFTTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKE
                     AANKMINTCNEYQKRHGKKTLFEVPEV"
BASE COUNT         1870 a         1605 c         1752 g         2050 t
ORIGIN      
        1 cactgatgcg ggtgccgggt ttgagcagac gctgaccgga atgacagtgg ggatcgacag
       61 ccgtaatgat attcctgagg ggattgcgac gctgggcgct tttatgggtt attcccattc
      121 acatatcggt tttgatcgtg gaggacatgg cagtgtggac agttattctc tgggcggcta
      181 tgccagttgg gaacatgaaa gtggtttcta tctggacggt gtcgtgaagc tgaaccgttt
      241 tgaaagtaac gtagccggta aaatgagcag cggtggagcc gccaatggca gttaccatag
      301 caacgggctg ggcggtcaca ttgaaaccgg gatgcgattt accgatggta actggaacct
      361 gacgccgtat gcctcgttaa cggggttcac cgctgataac cccgaatatc atttatccaa
      421 tggcatggaa tcgaaatcag tcgatacccg cagtatatat cgtgaactgg gtgcaacgct
      481 gagttacaac atgcgtctgg ggaacggtat ggaagttgag ccgtggctga aggcggctgt
      541 gcgcaaagaa tttgtcgatg ataaccgggt gaaagtgaat agtgacggta atttcgtcaa
      601 tgatttgtcg ggcagacgtg gaatatacca ggcaggtatt aaagcctcat tcagcagtac
      661 gttaagcggg catcttgggg tggggtatag caacggtgct ggtatggaat ccccgtggaa
      721 cgcggtggct ggtgtgaact ggtcgttctg accatcaacg attaaactgc gcttcggcgc
      781 agttttcgtt tacaggatgt taaaagagaa aattctgggg taaaaaaagc ccgccagtta
      841 cggcgggaaa cctcatccta tgggagaaca atgaataatg aaattgcggg gttatcatct
      901 cccagtatat caacactaac aataaggtta tttactcaac caggcataaa tattttgttt
      961 tgtgcgtggg aacagcctta aggtgtaaag ggggaggtgg aaatagcaat gaggagtatc
     1021 agcaagaata ctcgccgctt taccacaacg tggatgagag ggatgaaaaa ctcaaggcag
     1081 agataactct gccttgaaga taaatgcgct tttacagcgg gattatttca gctcttctgc
     1141 ttccggtaag gtcacgttca gctcaagaat agaaatatcg ccatcttttt gctcaagctg
     1201 tacggttacc atctcaggat caatttgtac gtatttacaa atgacctcaa gaatatcttt
     1261 acgcaactgc ggcagataat gcggttctgc atcgctgcga cggcgttcag caacaataat
     1321 ctgcagccgt tcttttgcaa tgttggctgt atttttcttc cgcgagagaa agaaatcgag
     1381 taatgccata acttatcctc cgaacaagcg tttgaggaag cctttcttct cttcttcaat
     1441 gaagcggaaa ggacgttctt ctcccaacag acgttctacg gtatctgcgt aggctttacc
     1501 cgcatcggcg ttaatgtcga gaatgaccgg ttcaccctgg ttagaggcgc gcaatactga
     1561 ttgatcctct gggatcacgc cgacgagttt gatgcgcagg atctccagca catcttccat
     1621 gctcagcatg tcacctctgc ttacgcggcc tgggttatag cgcgttaaca gcaggtgctc
     1681 tttaataggc tcttcgccat tttctgcgcg gcgtgatttt gacgccagaa tgcctaaaat
     1741 acggtcagag tcgcgtactg aggagacttc cgggttggtg gtaataatgg cttcgtctgc
     1801 aaaatagagt gccattaacg caccggtttc aatccctgcc ggggagtcac aaacgataaa
     1861 ttcaaaatcc atcgctttca gatcatcaag aactttagcg accccttcac gggtgagggc
     1921 atctttatcg cgtgtttgcg atgccggcag aatatagaga ttttcagtac gcttatcttt
     1981 aattaacgcc tgatttagcg ttgcatcgcc ctgaatgacg ttgacgaaat cgtaaacgac
     2041 ccggcgttca caacccataa tcaggtcgag attacgcagg ccgatatcaa aatctatcac
     2101 gacagttttc tttcccttct gggccaaacc agtggcgatg gccgcgctgg aggttgtctt
     2161 accaacaccc cctttgcccg aagtaacaac aataatgcgt gccatagaaa ttccttgtta
     2221 aaaagggatc aatttaacgg ttgaacggtc aaagcgtttt cgactaactg cagtcgcgcc
     2281 gctttgccat aaaattctgc tgggatttga tcactcagcc agtattcacc tgcgatggac
     2341 accaattccg ccatcaggtt cgtacaaaat atttgcgttt cccggtcacc acttgcccct
     2401 gccagcgcac gaccgcgcat catgccatag acatgaatgt tcccatcggc aatcaattcg
     2461 gccccagcgc taacgtggct tgtaacaatc agatcacatt gtggagcata aatacgctga
     2521 ccggaacgca ccggggtatc tattaaacgc gtttttgtga ccggcgttgt attttgcgct
     2581 ggagcctgcg gtgcgggagc tggacgtggc gctttttcct ttccttccgt caggataggc
     2641 agccccattt tttcaatttc ggctttaagt tgcgcatctt tgcagccact tacgccaata
     2701 acccgcaaac cggttgccga aaccgcctta tgcatcgctg accagtttac cgggtcttcc
     2761 agtgcactga cgttgagtac aacgggggca tgttttaaaa atgcgggggc ctgagcgatt
     2821 ttgtcttcca gcgcctgatg gataacctta ggttctgcct catgcagatg aaccacagat
     2881 aaagtgaagc tactgccttt aagctcgatt ggcgtgtttg acatcctggc cttactcaat
     2941 tagctattaa tcatcgccag cgcgcgatga tgttccgaag actataaggc atgttatagt
     3001 ctggattata ttgaggcaag tcaccctccc atttattcag agtaaaagtc tattctgtga
     3061 taaatggcgc tgattcatag cttaaaaaat acccttgtca atcaacccat tgccgtcgta
     3121 cttttgattc ttcttattta cgcttccttt ttccgcaccc taatcgtgac agcatctcca
     3181 gacctgttta aatatccagt attgaggcaa gtgtattttt tcaccacgcc aggctttctt
     3241 aactttccat tttttgcagc atcattaaag aacagatacc cgttatctta atatccgcca
     3301 cagaattgct ctatcataga aaccgcagaa gacgtctgat acttctgtca ggtcatcgta
     3361 actcgtccag gtcagcggtt ttgattacgg attgatgatg aaatgagcat aagtgttgta
     3421 cggttggatt atcaggatgt gctatgaata tgatgcgtat tttttatatc ggattgtcag
     3481 gtgtggggat gatgttctca tcaatggcgt ctggacatga cgctggcgga cttcaatctc
     3541 cggcgtgcgg cgttgtttgc gacccgtata tttgtgtgaa ctcagatggc atttccccag
     3601 agttaacaag gaaatatctc agcgaaaaag ccgctgaaaa cttacaatca ttacaaggct
     3661 acgatcccag cgaatttaca ttcgctaacg gtgtattttg cgatgttaaa gaaaaattat
     3721 gccgtgatga tcgttatttt ggtgtagatg gtaagcggag tggaaaaatc aatcaaacca
     3781 ccacaaagat gttatttatg tgtcgtgagt gatgtcttgt ttacattatg ttctattcgt
     3841 cattaacctc ttgagcgaca gagaggagtg gggcgtcaac gtttatgacg gggagactcc
     3901 ccgtcggttg acatttactt aatttgaata tcgacgttat attttttagt tttgttttta
     3961 cgggcgtcgt ttcgggtttg cagcactcgc agctcatatt tgccagaagc agggagtgag
     4021 tattgtccgt gactatcgag ttcaggggaa tatctggata aatcaacaga atcatcgatt
     4081 cctggcccga agaggtaagt atcagcgcct tcattagaga tactcacatg cactttctga
     4141 ccttttttgg catagaaggt atatgtatcg taatcgtatc cctttatttc gccggagtac
     4201 tgagcgctgc tatggccttt tctgaactca acattgacat tcttacccgc agcaaagctc
     4261 gtggaggtta gcagtgcaag cagcaataca gccttactga tgctcttgat tttcattggt
     4321 agtcttctgt tcagaatgag tacgatgaac tgttataata taacaatccc ttacgggaga
     4381 attccagtgg ctttcctgcg ttcggtaaaa tcccgtcgcg gcatttttgc cggggggatt
     4441 acttataaag gggcatggac tcaacaaacc gtagaaattt acagctggct ggcttgtggc
     4501 gcgggtttca ttgatgttag cttatgccaa aacctggcat cttaaagagt aaaagtatgt
     4561 tttgtgtgat ttatcgaagc agcaagcgtg accagaccta tttatatgtc gaaaaaaaag
     4621 acgatttttc gcgtgttcct gaggaactga tgaaaggttt tggtcagcct cagttagcga
     4681 tgattctgcc gctggatggg cggaagaaac tggtcaatgc cgatattgaa aaggttaaac
     4741 aggcattaac cgagcaaggt tactatttgc agttaccgcc accacccgaa gatttgctga
     4801 agcaacatct ttccgtcatg gggcagaaaa cagacgacac taacaaataa ccgatatccg
     4861 gcggtggcat tatctttgtc ggcgcgggtt ttcatatcca cgataaggtg aggggaacgt
     4921 tatgtatcaa catcacaact ggcaaggtgc gctgctggat tatccggtga gtaaagtagt
     4981 ttgtgttggc agtaactatg ccaaacatat taaagagatg ggcagcgcag tgcccgaaga
     5041 gccagtgctg tttattaaac cagaaacggc actgtgcgat ctgcggcagc cattggcgat
     5101 cccatccgat ttcggttcag ttcatcatga agtcgaacta gcggtgttga ttggcgcgac
     5161 gctgcgtcag gctacggaag agcatgtccg caaagccatt gccggttacg gcgtggcgct
     5221 cgatctgacg ttgcgtgatg ttcagggaaa aatgaagaaa gccgggcagc cgtgggaaaa
     5281 ggctaaagcg tttgataact cttgtccgct ttccgggttt attcccgcgg cggaattcac
     5341 cggcgatccg caaaatacaa cgctgggcct gagcgtaaac ggcgaacaac gccagcaagg
     5401 tacgactgcg gacatgatcc ataaaatcgt tccgctgatc gcttatatga gcaagttttt
     5461 taccctcaag gccggtgacg ttgtgctgac aggcacgcct gatggcgtcg gcccgttgca
     5521 aagcggtgat gagctgacag tcactttcga tgggcattct ttgacaactc gcgttttgta
     5581 atactttttg ccgcctgaaa gcggcggcaa aacttgcatc gctgtgccag actggttata
     5641 aggtgcgttt taacgtaatg gcggaacacc tgatgagcga tgtacctttc tggcaaagta
     5701 aaaccctgga cgaaatgagc gatgcggaat gggagtcgtt gtgtgatggt tgcggtcagt
     5761 gttgcctgca taaactgatg gatgaagaca ccgacgaaat ctacttcact aacgtcgcct
     5821 gtcgccagct caatattaaa acctgtcaat gtcggaacta cgaacgtcgt ttcgagtttg
     5881 aacccgactg cattaaatta acccgtgaaa atctgccaac attcgaatgg ctaccaatga
     5941 cctgcgctta tcgtttgctg gcggaaggta aagatttacc tgcgtggcat ccgctgctta
     6001 ctggttcgaa agcggcaatg catggtgaac gtatctctgt gcgtcatatc gcagtgaaag
     6061 aatcagaagt gattgactgg caggatcata tcttaaataa acctgactgg gcgcagtgat
     6121 aaatttaata gatgttgatg ggggctttct taatatacgt ctggatgctc gccatttcgt
     6181 agccctgttt ttgaaagcgc tctgtcataa tgagagttac ccgatacctt attatcaaca
     6241 ggtaactctc aatctttaaa aaacgtctgt atttttaaat gtgataaact ttataccttg
     6301 agtacatgga gagaataatt gctgatcaga cttcaggtac ctcaaagagt gtcttcttac
     6361 cgtgtctttt ctgatactca ttacaggtgt taatcatttt attggccgct tcttttagca
     6421 aagaaagcat taaatcatca taatcaacat agaatctggt tgtttcagtt tccgttttta
     6481 tctccccgat ggcggctatt tcggtggtta atttcaattt ggcggcatcg atatctttat
     6541 tcgcttgttt aaccgtgtta gacagggtgg taaagaaact ctgcacagat tttaacttgt
     6601 tcttcaattc tggaatcagt ttcccttcaa ctacgcccgc agcaatagaa taggaaatga
     6661 ttaaaccaaa tggaccggcg acgacaccgg ctgcggcacc ggcatacgct tccttcctga
     6721 ttttatctac ctgtgactgg aaatagctgc ttttttctga aaaatcattg gttaactggc
     6781 tatctaacgc cagcagtttc ccggaagcgt tgttgaaact ttgtgagctt accagcaggg
     6841 atttttgcgc ttcattcagc ttcgtgatgc cgtcatccag taccttaatg agaatgtctt
     6901 tctgggcgga tgctttcttc tcattatact catcaaatag caaaatatac gctgcgagca
     6961 attgcgtcgc aacaccacac cattcataca ctgtttgggt tgcttcaaaa tacttatcct
     7021 ggctatccat tagtaaggtt ttaatattgc caactaaaac ggaggctgcc tgtgaatact
     7081 cctgtttaaa gcgacttaac tcttttatgg tttcatcaaa ggtctgccag gggatgacct
     7141 gatcgagata tttattataa agatccaatg ctccatctgc ggtttcgatt gcgtttttaa
     7201 ctacctctac cgttttatct gcaacgattt cagtcataat cattcgcctc tttaaatata
     7261 taaattgtaa tgaaact
//
LOCUS       DBFIFI010000211         7269 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.211, whole genome shotgun sequence.
ACCESSION   DBFIFI010000211 DBFIFI010000000
VERSION     DBFIFI010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7269
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.211"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..386)
                     /gene="gpU"
                     /locus_tag="N7B39_004087"
     CDS             complement(<1..386)
                     /gene="gpU"
                     /locus_tag="N7B39_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail terminator protein"
                     /protein_id="HGU2423969.1"
                     /translation="MKHREIRAAVLSALKENISERVSWFDGRPVFIDEQELPAVAVYL
                     TDASAADEFVDEGTWEATLHIEVFLRAKEPDSALDMWMEEKILPALEAVPGLSALLLK
                     MNLQGYDYRRDDEFMMWGSADLLWKIT"
     gene            complement(399..1022)
                     /locus_tag="N7B39_004088"
     CDS             complement(399..1022)
                     /locus_tag="N7B39_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU2423970.1"
                     /translation="MAIKGLAQAMKNLDAIDRRAVPRASATTLNRVAGAIIAKTASSV
                     ARELAVPRRLIRARIRLSPARPDKVYAKVYINTGNLPAIKLGEARVRLSRRKRRKKGQ
                     RAALKGGGSVLIVGKRRIPDAFITRLANGRWHVMQRMPWASSSTGADSKGRPKRHRLP
                     IEVVKITTAGPLAETFERERDRMYREKLPAQMMKAMTHQLRLVLKRK"
     gene            complement(1025..1306)
                     /locus_tag="N7B39_004089"
     CDS             complement(1025..1306)
                     /locus_tag="N7B39_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HGU2423971.1"
                     /translation="MSDLFTRMCCRMDVATVRVMGKQAEINGVVYDVMPEEESAEMGA
                     LSGSQLSLVVFSARYRPARHDVVVFAGRTLTVTRYDTYNGKPRIFVEQE"
     gene            complement(1299..1625)
                     /locus_tag="N7B39_004090"
     CDS             complement(1299..1625)
                     /locus_tag="N7B39_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid cement protein"
                     /protein_id="HGU2423972.1"
                     /translation="MAKNFVQDGTTIELVNAGDQTILSGAAVVVGSMVAVAITDIPAG
                     EAGDGFAEGVFLLPKQSADDIQSGAVVYLKDGVVQLAADGAVAAGVAWENAPANSATV
                     AVKINV"
     gene            complement(1713..3737)
                     /locus_tag="N7B39_004091"
     CDS             complement(1713..3737)
                     /locus_tag="N7B39_004091"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HGU2423973.1"
                     /translation="MPELNDSISATPKASMKNKSWFRMQAGGPGDADIYIYDEIGFWG
                     VTAKQFVSELNALGDITHINLHINSPGGDVFEGIAIFNALKNQGATITVYVDGVAASM
                     ASVIAMAGDTVIMPENAFMMIHKPWGFSGGDAEDMRSYADLLDKVESVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAHGFADQVTPAVEAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRDTTVTIPAPAVTEPSPVPAVSDEATIRARVMAEQKARMSGINDLFAMF
                     GGRYQTLQAQCVADPDCSLEMARERLLNEMGKESSPTNKNTPAHIYAGNGNFVGDGIR
                     QAMLARAGFENVEKDNAYNGMTLREWARMSLTERGIGVASYNPMQMVGLALTHSTSDF
                     GNILLDVSNKGLIQGWEESEETFQKWTRKGRLSDFKTAYRVGMGGFGSLRQVREGAEY
                     KYITTSDRKETIALATYGEIFSITRQAIINDDLNMLVDVPMKMGRAAKATIGDLVYKV
                     LTDNPKLSDGKALFHADHKNIATGGISVSGLDAARQMMRLQKEGDRALNIRPAFMLVP
                     VALETVANQTIKSASVKGADANAGVINPIQNFAEVIAEARLDAADPKTWYLAAAQGTD
                     TIEVAWLDGVDTPYIDQQEGFTTDGIATKIRIDAGVAPLDWRGLVRSSVA"
     gene            complement(3682..5184)
                     /locus_tag="N7B39_004092"
     CDS             complement(3682..5184)
                     /locus_tag="N7B39_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU2423974.1"
                     /translation="MAIIDDVIGVFSPGWKAARLRSRALIMAYEAVKPTRTHKARREN
                     RSADQLSKYGAVSLREQARFLDINHDLVIGVFDKLEERVIGARGIIVEPQPLRKNGEM
                     AAELAADIRRLWAEWSVSPDVTGQYTRPVLERLLLRTWLRDGEVFAQMVSGAGNGLER
                     TAGVPFWLEAMEPDFVPMRTDESAGLNQGVFLDEWGRPKKYLVYKNYPVSGRQSDTKE
                     IAAGKMIHLKFTRRLHQTRGSSMLSGVLMRISALKEYEDAELTAARIAAALGLYIRKG
                     DGQDYEDPGIKETEREVHITPGIIYDDLRKGEDIGMVKSDRPNPNLETFRNGQLRAVA
                     AGSRLSFSSAARNYNGTYSAQRQELVESTDGYLILQDCFIGAVTRPVYRTWLNMVVAA
                     GLLKIPADVEMKTLYNATYSGPVMPWIDPVKEAEAWRIQIRGGAATESDWVRAGGRNP
                     DEVKRRRKAEIDENSRLGLVFDTDPVNDKGGNSAGTERQYQRDTESQHEE"
     gene            complement(5184..5396)
                     /locus_tag="N7B39_004093"
     CDS             complement(5184..5396)
                     /locus_tag="N7B39_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2423975.1"
                     /translation="MSTEEAREMIQRYREAEMAVLEGKSVIFNGQQLTLESLSQIRAG
                     RQEWERRLAAMVSRRRGKPGFKLARF"
     gene            complement(5393..6517)
                     /locus_tag="N7B39_004094"
                     /pseudo
     CDS             complement(5393..6517)
                     /locus_tag="N7B39_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310990.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
     gene            complement(6889..7170)
                     /locus_tag="N7B39_004095"
     CDS             complement(6889..7170)
                     /locus_tag="N7B39_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2423976.1"
                     /translation="MTEAEILGLIRRVAGISQQVDEQATQPDSMTADNYVRVVVEVMR
                     RDGIQLNDVDMRNIRTRVLELLAYRRRSQQRRESAKNTYQWKTPERLRR"
BASE COUNT         1593 a         2268 c         1696 g         1712 t
ORIGIN      
        1 gtaattttcc acaggagatc tgccgatccc cacatcataa actcatcatc ccggcggtag
       61 tcatacccct gaagattcat cttcagcagt aatgcactga gcccgggaac tgcctccagc
      121 gcaggaagaa ttttttcttc catccacata tccagtgccg agtccggttc ttttgccctg
      181 agaaaaacct caatatgcag tgtcgcctcc caggttccct catcaacgaa ctcgtcagca
      241 gcagacgcat ctgtcaggta aacagcaaca gcaggcagtt cctgttcatc aataaaaacc
      301 gggcgaccgt caaaccagct caccctctca gaaatatttt ctttcagggc agacagaact
      361 gccgcccgta tttcacggtg tttcatacac cctcccagtc attttctttt cagcaccagg
      421 cgtaactgat gcgtcatggc tttcatcatc tgcgccggta atttttcccg gtacatccgg
      481 tcccgttcac gttcaaaggt ttctgccagc ggtccggcag tcgtaatctt caccacttcg
      541 atcggcagac ggtggcgttt cggcctccct ttgctgtccg cgccggtgga tgatgatgcc
      601 cacggcatac gctgcatcac atgccagcgt ccgttagcca gccgggtgat aaaggcgtcc
      661 gggatccgtc ttttccccac aatcagcaca ctgccgcccc ctttcagggc cgcacgctgt
      721 cctttctttc tccgttttct gcgggaaagt cgaacgcggg cctcccccag tttgatggcg
      781 ggcaggttgc cggtattgat gtaaaccttt gcgtaaacct tatccggtcg tgccggactt
      841 aaccggatgc gggcacggat aagacgacgg ggaacggcca gctccctggc aactgaagag
      901 gccgttttcg caataatggc ccccgccact cggttcagtg tcgtggcaga ggcccgggga
      961 acggcacggc gatcaattgc atccagattt ttcatggcct gcgccagacc ttttattgcc
     1021 atactcattc ctgttcgaca aaaatccggg gtttaccgtt gtacgtgtca taacgggtca
     1081 ccgtcagtgt gcgccccgca aacacaacaa catcatgacg ggccggacgg taccgggctg
     1141 aaaacaccac cagtgacaac tggctgcccg aaagcgcccc catctccgcg gactcttcct
     1201 ccggcatcac gtcgtacacg acgccgttaa tctccgcctg tttgcccatc acccgaacgg
     1261 tcgccacgtc catccggcaa cacattcgcg taaacagatc agacattgat ttttaccgcc
     1321 acagtggcgc tgtttgcagg ggcattttcc caggctaccc ccgctgccac cgcaccgtct
     1381 gcagccagct gcacaacccc gtccttcaga taaaccaccg cgccggactg aatgtcgtca
     1441 gcagactgtt tgggcagcag gaacacgcct tcggcaaaac cgtcaccggc ctcaccggca
     1501 ggaatatcgg taatggccac ggccaccata ctgccgacca ccaccgcagc accgctcagg
     1561 atggtctgat ctccggcatt caccagttca atggtggtac cgtcctgtac aaaatttttc
     1621 gccataatgc tgtttctccg gacagcccct gtggggctgt ttttcaggca taaaaaaagc
     1681 cctttcgggc agtgattgtg ataacgcggt tatcaggcca ccgacgaacg caccagcccg
     1741 cgccagtcaa gtggtgccac tccggcatca atacggattt ttgtggcaat gccgtcagtg
     1801 gtgaaacctt cctgctgatc aatgtatggc gtgtccacac catccagcca ggccacttca
     1861 atggtgtcag tgccctgtgc cgccgccaga taccaggttt tcgggtctgc cgcatcaaga
     1921 cgcgcttctg caatcacctc agcaaagttc tggatagggt taatgacacc ggcgtttgca
     1981 tccgcccctt tcacactggc cgatttgatg gtctggttcg ccaccgtctc cagtgccacc
     2041 ggtaccagca taaaggccgg acggatattc agggcacgat cgccttcttt ctgcaggcgc
     2101 atcatctgac gggccgcatc cagtccggaa acggagatcc ccccggtggc aatatttttg
     2161 tgatcggcat ggaacagcgc cttaccgtcg gacagtttcg ggttatccgt cagcaccttg
     2221 tagaccaggt caccaatcgt tgccttcgcc gcacgcccca tcttcatcgg cacgtccacc
     2281 agcatattca gatcatcatt gataatggcc tggcgggtga tggagaaaat ctccccgtaa
     2341 gtggccagtg caatggtctc cttgcgatct gaggtggtga tgtatttata ctccgccccc
     2401 tcacgaacct ggcgcagaga accaaaaccg cccatcccca cgcgatacgc tgttttgaag
     2461 tctgacaggc gtcccttacg ggtccacttc tggaaggttt cttctgattc ctcccagccc
     2521 tggatcagcc ccttgttcga cacatccagc agaatattgc caaaatcaga ggtgctgtgc
     2581 gtcagcgcca gcccgaccat ctgcatgggg ttataactgg ccaccccaat accgcgctcc
     2641 gtcagtgaca tgcgagccca ttcacgcagg gtcatcccgt tataggcgtt atccttctcg
     2701 acattttcaa atccggcacg ggccagcatc gcctggcgga tcccgtcccc cacaaaattg
     2761 ccgtttccgg cataaatatg ggccggtgta tttttgttgg tcggcgagga ctccttgccc
     2821 atttcattca gcagacgttc acgggccatt tccagcgaac agtcaggatc agccacgcac
     2881 tgtgcctgaa gcgtctgata gcgaccgccg aacatggcaa acagatcgtt aatgcctgac
     2941 atgcgggctt tctgctcagc cataacgcgg gcgcgaatgg tcgcctcatc agacactgcc
     3001 ggtaccggtg atggttctgt taccgccggt gcagggattg tcactgtggt atcacgcggg
     3061 gcactgttgc gtggcggagt aatcatgttt cggatggatt ccggcatctt tttaaattcc
     3121 tctgtacgtt ttgactgaat acatgccatt gcctcaacag cgggtgtcac ctggtcagca
     3181 aatccgtgtg ccagacattc ggcaccggac atccaggttt catccgccag catggcggca
     3241 atttcatcgg tggtttttcc ggttttctgc gcataggctg gcaacagtac cgattcgact
     3301 ttatccagca aatcggcata actgcgcata tcctcagcat ccccgccact gaatccccat
     3361 ggcttatgga tcatcatgaa ggcattttcc ggcataatga ccgtatcacc ggccatcgca
     3421 atcacagatg ccatcgaggc ggcaacgcca tccacataca cggtaatggt cgccccctga
     3481 tttttcaggg cattaaaaat ggcgatgcct tcaaagacat cgccacccgg tgaattgata
     3541 tggagattaa tgtgggtgat atcacccagt gcattcagtt cgctgacaaa ctgcttcgcg
     3601 gtaactcccc agaaaccaat ctcgtcataa atataaatat ccgcgtcacc cggcccccca
     3661 gcttgcatcc tgaaccagga tttattcttc atgctggctt tcggtgtcgc gctgatactg
     3721 tcgttcagtt ccggcactgt tgcctccttt gtcgttgacg gggtcagtat caaagaccag
     3781 ccccagtctg ctgttttcat caatttcagc cttgcggcga cgtttgacct catccggatt
     3841 gcgcccaccg gcacgcaccc agtcagattc tgtcgctgca ccaccccgga tctgaattct
     3901 ccaggcttca gcttccttaa ccgggtcgat ccacggcatc accggaccgg aatacgtcgc
     3961 gttatatagc gttttcatct ccacatccgc cggaattttc agcagacctg ccgcaaccac
     4021 catattcagc catgtccggt acaccgggcg ggttaccgcg ccaataaaac agtcctgcag
     4081 gatcaggtaa ccatccgtgg actcgaccag ctcctgccgc tgggcgctgt aggtgccgtt
     4141 atagttacgc gccgcactgg aaaaactcag acgactgcct gctgccactg cacgcaactg
     4201 gccgttgcgg aaagtttcaa ggttgggatt gggacggtca gatttgacca tgccgatatc
     4261 ctcgcccttg cgcaaatcgt cataaataat acccggggtg atatggactt cccgctcggt
     4321 ctctttgatc cccggatctt catagtcctg tccgtcacct ttacggatat acagtcccag
     4381 cgccgcagca atacgcgccg ctgtcagttc cgcatcctca tactccttaa gggcactgat
     4441 ccgcatcagc acccccgata acatggatga gcctcgcgtc tgatgcagac gacgagtgaa
     4501 cttcaggtgg atcatttttc cggcagcgat ttctttcgta tcactctgcc ggccgctgac
     4561 cggataattt ttataaacca gatatttttt cggtcttccc cactcatcaa gaaaaacccc
     4621 ctgattcagt ccggcggatt catcagtgcg catgggaaca aaatccggct ccatcgcctc
     4681 aagccagaat ggcactcccg ccgtccgttc cagaccgttt cccgcaccac tgaccatctg
     4741 cgcaaacact tcaccatccc gcagccaggt ccgcagcagt aaacgttcaa gcacaggacg
     4801 ggtatactgc cctgtcacat ccggactcac ggaccattca gcccacaaac ggcggatatc
     4861 cgcagccagc tcagccgcca tttccccgtt ttttcgtaat ggctgaggct ccacaataat
     4921 tcccctggca ccaatcaccc gctcttccag cttgtcaaac acaccaatca ccaggtcatg
     4981 attgatatcc agaaaacggg cctgctcccg cagggaaacc gcaccgtatt tactgagctg
     5041 atcagcagag cgattttccc gccgggcttt atgtgtccgg gtcggtttca ccgcctcata
     5101 ggccatgatt aacgcccttg aacgcagtct ggctgctttc cacccggggg aaaacacgcc
     5161 gatcacatca tcaataattg ccattaaaac ctcgccagtt taaatcccgg ttttccccgc
     5221 ctgcggctca ccatcgcggc aagcctgcgt tcccactcct gacgtccggc gcggatctga
     5281 gaaaggcttt ccagcgtcag ttgctgcccg ttgaagatga cagactttcc ctccagtacg
     5341 gccatttccg cttcacggta ccgctgtatc atttctctgg cttcttctgt gctcacaacc
     5401 agcctcctga tgttatccat ggattatctt ccgcacgctc cgtccgcagt tttttcttcc
     5461 ggcgacggcg tttttctgcc ccggccgtca gttccgggga taccgtttca ccagaacgct
     5521 cctgcgggaa gacgagccac gtttcccgct gtgcccagtc cggtgcggag ggccagcgga
     5581 tcttttcgta accatgcaga acggcaagcg catccgcata aaccagcagg tcaaacgctt
     5641 cgttagcgcc cctgcccggt tttcgccatt ttccgtcact gccgcgctct tcataggtca
     5701 gctcatcgta aaaccaccgc cccagccagt cgggaaagtg gatatagttc ggccctggtg
     5761 tgtcacgcca cagggcatta tttacacgat ccttaaacgc atccgtctga accagccaca
     5821 gcgcgacatc gccactggct ctggcacggc gggcacttct gccggtatta tccgggaagg
     5881 ttcggttaat cagcctgtca cggcgaagtc catccccctt gaacagaaac accctgttgc
     5941 ccagtccgtc actccggcaa cgacgccaga aacgataggc gttatctgtc accccggctt
     6001 cccctcccgt atccaccgcc atggccatca gacgcatgcg cacatccgga tcagaagcca
     6061 gcggccatgt tttatggaac acatccgtca gcaacaaatc ccagtcctcc ggatatgccg
     6121 ccggatcaac cggcagactt tcaccgttgg gactgcagcg cagtgaatgc cggatgttgt
     6181 agcgatcaac aatccagcgt tccccctgct ctccgtatcc ggtgatctgc acaacaaaac
     6241 ggcgattttt accgccctgt acgtcaaccg ttgcctcaat aaaacgcaca ccatccggca
     6301 cagatcgccg gggaaacggc tcggcacgct gttcaagcag ttcactttta cgctgttccg
     6361 tggctgaacg gggcagatag ggtcgtccga tatcggtgtt caccaccgct ttcagggtct
     6421 cttcactgcc ggttcgctca tactcttctt ctgccgccag cagtttaaaa atcagttgtt
     6481 cccaggtctg aaacgccgca gctggcccct ccatccaaga agaagtaaat aaaaggtaac
     6541 taaatgatta taaggtacta ttgagttatg ccccctacaa catagtccca gatttagtac
     6601 ctgcttaagc gataaaaaag caaaaaagga agcaatatta attattgccg ctatgcatga
     6661 tcacaatgac aaatcagagg cggaattccg ctctcggaac tcacatatga gaaacgatat
     6721 ttcttccacc gatacccttt atttgggtat cgcaagtaag ccccaaaatc tgggtgttgc
     6781 tcttactacc caaactacgg gtagtttccg taatttcatg atcgagttgc agatctgcaa
     6841 ctcccccacc agccaacgca attttgcgtt atcgtgaata tcaacaagtt acctccgcag
     6901 ccgttccggc gtcttccact ggtaagtatt tttcgcgctc tccctccgtt gttgagaacg
     6961 gcgacggtat gccagcaact caaggactct ggttcgtata ttgcgcatat ccacatcatt
     7021 aagctggata ccatcacggc gcatcacctc caccactaca cgcacataat tatctgcggt
     7081 catgctgtcc ggctgcgtgg cctgttcgtc aacctgctgg ctgattccgg cgacgcggcg
     7141 gattaatccc agtatttcgg cttctgtcat tgtgccccca tcgcgctggt gaagaaaaca
     7201 agctcagatt ttttgtaaag aatctgtcac gctaaaagat gtcgaacaaa aaacaacctt
     7261 cttcatcat
//
LOCUS       DBFIFI010000212         7259 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.212, whole genome shotgun sequence.
ACCESSION   DBFIFI010000212 DBFIFI010000000
VERSION     DBFIFI010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7259
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.212"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            196..1530
                     /gene="cadB"
                     /locus_tag="N7B39_004096"
     CDS             196..1530
                     /gene="cadB"
                     /locus_tag="N7B39_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HGU2423977.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            1610..3757
                     /gene="cadA"
                     /locus_tag="N7B39_004097"
     CDS             1610..3757
                     /gene="cadA"
                     /locus_tag="N7B39_004097"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HGU2423978.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            3816..5273
                     /gene="dtpC"
                     /locus_tag="N7B39_004098"
     CDS             3816..5273
                     /gene="dtpC"
                     /locus_tag="N7B39_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HGU2423979.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESH
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMVSGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMVQESND"
     gene            5510..7027
                     /gene="lysS"
                     /locus_tag="N7B39_004099"
     CDS             5510..7027
                     /gene="lysS"
                     /locus_tag="N7B39_004099"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HGU2423980.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(7146..>7259)
                     /gene="ghoT"
                     /locus_tag="N7B39_004100"
     CDS             complement(7146..>7259)
                     /gene="ghoT"
                     /locus_tag="N7B39_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HGU2423981.1"
                     /translation="IIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF"
BASE COUNT         1783 a         1698 c         1773 g         2005 t
ORIGIN      
        1 atatcaactt gcgatcctga tgtgttaata aaaaacctca agttctcact tacagaaact
       61 tttgtgttat ttcacctaat ctttaggatt aatccttttt tcgtgagtaa tcttagcgcc
      121 agtttggtct ggtcaggaaa tagttataca tcatgacccg gactccaaat tcaaaaatga
      181 aattaggaga agagcatgag ttctgccaag aagatcgggc tatttgcctg taccggtgtt
      241 gttgccggta atatgatggg gagcggtatt gcattattac ctgcgaacct agcaagtatc
      301 ggtggtattg ctatctgggg ttggattatc tctattattg gtgcaatgtc gctggcgtat
      361 gtatatgccc gactggcaac aaaaaacccg caacaaggtg gcccaattgc ttatgccgga
      421 gaaatttctc ctgcatttgg ttttcagaca ggtgttcttt attaccatgc taactggatt
      481 ggtaacctgg cgattggtat taccgctgta tcttatcttt ccaccttctt cccagtatta
      541 aatgatcctg ttccggcggg tatcgcctgt attgctatcg tctgggtatt tacctttgta
      601 aatatgctcg gcggtacctg ggtaagccgt ttaaccacta ttggtctggt gctggttctt
      661 attcctgtgg tgatgactgc tattgttggc tggcattggt ttgatgcggc aacttatgca
      721 gctaactgga atactgcgga taccactgat ggtcatgcga tcattaaaag tattctgctc
      781 tgcctgtggg ccttcgtggg tgttgaatcc gcagctgtaa gtactggtat ggttaaaaac
      841 ccgaaacgta ccgttccgct ggcaaccatg ctgggtactg gtttagcagg tattgtttac
      901 atcgctgcga ctcaggtgct ttccggtatg tatccgtctt ctgtaatggc ggcttccggt
      961 gctccgtttg caatcagtgc ttcaactatc ctcggtaact gggctgcgcc gctggtttct
     1021 gcattcaccg cctttgcgtg cctgacttct ctgggctcct ggatgatgtt ggtaggccag
     1081 gcaggtgtac gtgccgctaa cgacggtaac ttcccgaaag tttatggtga agtcgacagc
     1141 aacggtattc cgaaaaaagg tctgctgctg gctgcagtga aaatgactgc cctgatgatc
     1201 ctcatcactc tgatgaactc tgccggtggt aaagcatctg acctgttcgg tgaactgacc
     1261 ggtatcgcag tactgctgac tatgctgccg tacttctact cttgcgttga cctgattcgt
     1321 tttgaaggcg ttaacatccg caactttgtc agcctgatct gctctgtact gggttgcgtg
     1381 ttctgcttca tcgcgctgat gggcgcaagc tccttcgagc tggcaggtac cttcatcgtc
     1441 agcctgatta tcctgatgtt ctacgctcgc aaaatgcacg agcgccagag ccactcaatg
     1501 gataaccaca ccgcgtctaa cgcacattaa ttaaaagtat tttccgaggc tcctcctttc
     1561 attttgtccc atgtgttggg aggggccttt tttacctgga gatatgacta tgaacgttat
     1621 tgcaatattg aatcacatgg gggtttattt taaagaagaa cccatccgtg aacttcatcg
     1681 cgcgcttgaa cgtctgaact tccagattgt ttacccgaac gaccgtgacg acttattaaa
     1741 actgatcgaa aacaatgcgc gtctgtgcgg cgttattttt gactgggata aatataatct
     1801 cgagctgtgc gaagaaatta gcaaaatgaa cgagaacctg ccgttgtacg cgttcgctaa
     1861 tacgtattcc actctcgatg taagcctgaa tgacctgcgt ttacagatta gcttctttga
     1921 atatgcgctg ggtgctgctg aagatattgc taacaagatc aagcagacca ctgacgaata
     1981 tatcaacact attctgcctc cgctgactaa agcactgttt aaatatgttc gtgaaggtaa
     2041 atatactttc tgtactcctg gtcacatggg tggtactgca ttccagaaaa gcccggtagg
     2101 tagcctgttc tatgatttct ttggtccgaa taccatgaaa tctgatattt ccatttcagt
     2161 atctgaactg ggttctctgc tggatcacag tggtccacac aaagaagcag aacagtatat
     2221 cgctcgcgtc tttaacgcag accgcagcta catggtgacc aacggtactt ccactgcgaa
     2281 caaaattgtt ggtatgtact ctgctccggc aggcagcacc attctgattg accgtaactg
     2341 ccacaaatcg ctgacccacc tgatgatgat gagcgatgtt acgccaatct atttccgccc
     2401 gacccgtaac gcttacggta ttcttggtgg tatcccacag agtgaattcc agcacgctac
     2461 cattgctaag cgcgtgaaag aaacaccaaa cgcaacctgg ccggtacatg ctgtaattac
     2521 caactctacc tatgatggtc tgctgtacaa caccgacttc atcaagaaaa cactggatgt
     2581 gaaatccatc cactttgact ccgcgtgggt gccttacacc aacttctcac cgatttacga
     2641 aggtaaatgc ggtatgagcg gtggccgtgt agaagggaaa gtgatttacg aaacccagtc
     2701 cactcacaaa ctgctggcgg cgttctctca ggcttccatg atccacgtta aaggtgacgt
     2761 aaacgaagaa acctttaacg aagcctacat gatgcacacc accacttctc cgcactacgg
     2821 tatcgtggcg tccactgaaa ccgctgcggc gatgatgaaa ggcaatgcag gtaagcgtct
     2881 gatcaacggt tccattgaac gtgcgatcaa attccgtaaa gagatcaaac gtctgagaac
     2941 ggaatctgat ggctggttct ttgatgtatg gcagccggat catatcgata cgactgaatg
     3001 ctggccgctg cgttctgaca gcacctggca cggcttcaaa aacatcgata acgagcacat
     3061 gtatcttgac ccgatcaaag tcaccctgct gactccgggg atggaaaaag acggcaccat
     3121 gagcgacttt ggtattccgg ccagcatcgt ggcgaaatac ctcgacgaac atggcatcgt
     3181 tgttgagaaa accggtccgt ataacctgct gttcctgttc agcatcggta tcgataagac
     3241 caaagcactg agcctgctgc gtgctctgac tgacttcaaa cgtgcgttcg acctgaacct
     3301 gcgtgtgaaa aacatgctgc cgtctctgta tcgtgaagat cctgaattct atgaaaacat
     3361 gcgtattcag gaactggctc agaatatcca caaactgatt gttcaccaca atctgccgga
     3421 tctgatgtat cgcgcatttg aagtgctgcc gacgatggta atgactccgt atgctgcatt
     3481 ccagaaagag ctgcacggta tgaccgaaga agtttacctc gacgaaatgg tcggtcgtat
     3541 taacgccaat atgatccttc cgtatccgcc gggagttcct ctggtaatgc cgggtgaaat
     3601 gatcaccgaa gaaagccgtc cggttctgga gttcctgcag atgctgtgtg aaatcggcgc
     3661 tcactatccg ggctttgaaa ccgatattca cggtgcatac cgtcaggctg atggccgcta
     3721 taccgttaag gtattgaaag aagaaagcaa aaaataatta gctcgtacaa gggaagtggc
     3781 ttgccacttc ccttttttgc tcataaggag aacacatgaa aacaccctca cagccgcgcg
     3841 cgatatacta tatcgtggcg atccaaatct gggagtactt cagtttttac ggcatgcgtg
     3901 ccttactcat tctctatctc acccatcagc ttggttttga tgataaccat gccatcagcc
     3961 tgttcagcgc atatgcttct ctggtttacg ttacccctat tctcggcggc tggcttgccg
     4021 accgcctgct cggcaaccgc actgcagtga ttgccggcgc gctgttaatg acccttggcc
     4081 atgtggtgct gggtattgat acaaattcaa cctttagcct gtatctggcg ctggcaatca
     4141 ttatttgtgg ctacggttta ttcaaatcaa acatcagctg tttgcttggc gagctctacg
     4201 acgagaacga tcatcgacgt gatggcggtt tttcgctgct gtatgctgcg ggcaatatcg
     4261 gttctatcgc cgccccaatc gcctgcggcc tggctgctca gtggtatgga tggcatgttg
     4321 gctttgccct tgcgggtggc ggcatgttta tcggtttgtt gattttctta agcgggcatc
     4381 gtcatttcca gtccacgcgt agtatggata aaaaagcgct cactagcgtc aaatttgcct
     4441 taccggtatg gagctggtta gtggtgatgc tctgtttagc cccagtattt tttaccctgc
     4501 tgctggagaa cgactggtcc ggatatttgc tggcgatcgt ttgcctcatt gccgcacaaa
     4561 tcattgcccg catgatgatc aaattccccg aacaccgccg tgctctttgg caaattgtat
     4621 tgttgatgtt tgtcgggaca ttgttctggg tactggcaca acagggcggc agtaccatca
     4681 gcttgtttat cgatcgcttt gtgaatcgtc aggcattcaa tattgaagta cctactgcac
     4741 tattccagtc ggtgaatgca attgcggtga tgctcgctgg ggttgttctg gcctggctgg
     4801 cgtcgccaga aagccacggc aactcaacat tgcgcgtctg gctgaagttt gcctttggct
     4861 tactgctgat ggcttgtggc tttatgttgc tggcatttga tgcccgacat gcagcagctg
     4921 acggtcaagc gtcaatgggc gtgatggtat ccgggctggc gctaatgggc tttgccgaac
     4981 tctttattga cccggtggcg attgcgcaaa tcacgcgtct gaaaatgtct ggcgtattaa
     5041 ccgggattta tatgctggca acaggcgcgg tcgccaactg gctggcaggc gtagtggcac
     5101 agcagacgac agagtcgcaa attagcggta tggcaattgc agcttaccag cgattctttt
     5161 ctcagatggg agagtggacg ttggcttgtg tcgcgatcat cgtggtattg gcctttgcta
     5221 cccgtttcct gtttagcacg ccgacgaata tggtacagga gagcaacgat taactgtcgt
     5281 aaaaacaatt ggttatgaat aaattaactt tcagtttatc acactagtaa atcgagatta
     5341 ataagtcatt taaaccattt tgatggttat ttattagtga tatcaacttg aggtaagcgt
     5401 tagtttcgat aagataaact gagttattaa tagtcgaggc agataataca gtgtaccgat
     5461 ctgtctcttt atctacgcta aattgaaagc tggatttaga ggaaccaaaa tgtctgaaca
     5521 agaaacacgg ggagccaatg aggctattga ttttaacgat gaactgagaa atcgccgcga
     5581 aaaactggcg gcactacgtc agcaaggtgt ggcgtttccc aatgattttc gccgcgacca
     5641 tacctctgac cagttgcacg aagagtttga tgcgaaggat aaccaggaac tggaatcctt
     5701 aaacattgaa gtctcggttg ctggccgaat gatgacccgt cgtatcatgg ggaaagcctc
     5761 ctttgtaacg ttgcaggatg tcggtggccg tattcaactg tacgtcgcac gagatagcct
     5821 gccagaaggt gtttataacg atcagtttaa aaaatgggat ctgggtgaca ttatcggtgc
     5881 ccgcggtacg ctgtttaaga cgcaaacggg tgagctttcc attcactgta ctgagttgcg
     5941 cctgctgact aaagcactac gtcctttacc agataaattc catggtctgc aggatcagga
     6001 agtccgttat cgtcaacgtt atctggacct catcgctaac gataaatccc gtcaaacgtt
     6061 tgttgtccgt tcaaaaattc tggccgctat ccgccagttc atggtcgcgc gcggctttat
     6121 ggaagtagaa accccgatga tgcaggtaat tccaggtggg gcatctgctc gcccgtttat
     6181 tacccatcat aatgctctgg atttagatat gtatctgcgt atcgcgccgg agctgtatct
     6241 gaaacgtctg gttgtaggcg gttttgaacg ggtattcgaa atcaaccgta acttccgtaa
     6301 tgaaggtatt tctgttcgcc ataatcctga gttcacaatg atggaactct acatggcgta
     6361 tgcggattac cacgatttga ttgaactgac agagtccctg ttccgcaccc tggcacaaga
     6421 ggttctgggt accactaaag tcacttatgg cgagcatgtg tttgatttcg gcaaaccgtt
     6481 tgaaaaactc accatgcgcg aagcaatcaa aaaatatcgt ccagaaaccg atatggccga
     6541 cctggataat tttgatgctg ctaaagcatt agctgaatct atcggtatta cggtagagaa
     6601 aagctggggg ctgggacgta ttgtcacgga gatctttgat gaagtggcag aagcacatct
     6661 gattcagcca acctttatta cggaatatcc ggcagaagtg tcaccgctgg cacgccgtaa
     6721 tgatgttaac ccggaaatca ccgaccgttt tgaattcttc atcggtggtc gtgaaatcgg
     6781 taatggtttt agcgaattaa acgacgcaga agatcaggct gaacgtttcc aggaacaggt
     6841 taacgctaaa gccgcaggtg acgacgaagc catgttctat gacgaagatt acgtgactgc
     6901 gctggaatat ggtctgccgc caaccgctgg tctgggtatt ggtatcgacc gaatgattat
     6961 gctgtttact aacagccata ctattcgcga cgttattctc ttcccggcga tgcgcccaca
     7021 gaaataaatt tcactttaat gaacgaagca gtcaggcgac tgcttcgttc atcgttccgc
     7081 aaatccagtt ttgaggagag acgcttcgtt tcacgataac cagcgggtcc actcaactac
     7141 agctcctaaa agagagaaaa aagtaatgcc acaggcagac tcattggcca ggttattccg
     7201 accaggaatg cactaagtaa acgaatatgt gttttctcat gtgagataaa ccagataat
//
LOCUS       DBFIFI010000213         7248 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.213, whole genome shotgun sequence.
ACCESSION   DBFIFI010000213 DBFIFI010000000
VERSION     DBFIFI010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7248
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.213"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            93..611
                     /locus_tag="N7B39_004101"
     CDS             93..611
                     /locus_tag="N7B39_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HGU2423982.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTDPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            821..2962
                     /locus_tag="N7B39_004102"
     CDS             821..2962
                     /locus_tag="N7B39_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HGU2423983.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLALSLVSQQ
                     FLSLEFQQILDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQKLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIASILGTILQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVLYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     RGSGTTLDNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAEKIGNNQAITVTNNQILNIGVNQIQTVGVNQVETVGSNQIIKVGSNQV
                     EKVGIIRALTVGVAYQTTVGGIMNTSVALLQSSQVGLHKSLMVGMGYSVNVGNNVTFS
                     VGKTMKENTGQTAVYSAGEHLELCCGKARLVLTKDGSIFLNGTHIHLEGESDVNGDAP
                     VINWNCGATQPVPDAPVPKDLPPGMPDMRQF"
     gene            3038..>7248
                     /locus_tag="N7B39_004103"
     CDS             3038..>7248
                     /locus_tag="N7B39_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element core protein"
                     /protein_id="HGU2423984.1"
                     /translation="MGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGIT
                     YANPVNPVLGAKVLPGETDLALPGPLPFILSRAYSSYRTRTPAPVGVFGPGWKAPFDI
                     RLQIRDEGLILNDNGGRSIHFEPLFPGEISYSRSESFWLARGGVAEQHSSQPLSALWQ
                     VLPEDVRLSPHMYLATNSLQGPWWILNWPERVPGADEVLPPEPPAYRVLTGVVDGFGR
                     TLAFHRAAEGDVAGAVTGVTDGAGRRFHLVLTTQAQRAEVFRKQRATSLSSPAGPRSA
                     SSSLVFPDTLPAGTEYGADNGIRLEAVWLTHDPAYPDELPAAPLARYTYTASGELRAV
                     YDRSGTQVRGFAYDAEHAGRMVAHHYAGRPESRYRYDDTGRVTELVNPEGLDYRFEYG
                     QDRVTITDSLNRREVLYTEGEGGLKRVVKKEHADGSITRSEYDEAGRLKAQTDAAGRR
                     TEYSLHMASGAVTAVTGPDGRTVRYGYNSQRQVTSVTYPDGLRSSREYDEKGRLAAET
                     SRSGETTRYSYDDPASELPTGIQDATGSTKQMAWSRYGQLLTFTDCSGYTTRYEYDRY
                     GQQIAVHREEGISTYSSYNPRGQLVSQKDAQGRETRYEYSAAGDLTAIVAPDGSRSEI
                     QYDAWGKAVSTTQGGLTRSMGYDAAGRITVLTNENGSQSTFRYDPVDRLTEQRGFDGR
                     TQRYHYDLTGKLTQSEDEGLVTLWHYDASDRITHRTVNGDPAEQWQYDEHGWLTTLSH
                     TSEGHRVSVHYGYDDKGRLTGERQTVENPETGEMLWEHETGHAYSEQGLATRQEPDGL
                     PPVEWLTYGSGYLAGMKLGGTPLVEYMRDRLHRETARSFGGEAYELATAWNTSGQLRS
                     RHLNLPQLDRDYDWNDNGQLIRISGPQESREYRYSDTGRLTGVHTTAANLDIDIPYAT
                     DPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYIYRYDEYGRLAEKTDRIPEGVIRMHD
                     ERTHHYHYDSQHRLVFHTRIQHGEPQVESRYLYDPLGRRTGKRVWRRERDLTGWMSLS
                     RKPEETWYGWDGDRLTTVQTQQTRIQTVYQPGSFTPLLRIETENGEQAKARHRSLAEV
                     LQEDTGVTLPAELAVMLGRLERELRQGSVSEESQQWLAQCGLTAEQMAAQLEAEYIPE
                     RKLHLYHCDHRGLPLALISPEGETAWQGEYDEWGNLLGETSAQHLQQSLRLPGQQYDE
                     ELGLYYNRNRYYDPLQGRYITQDPIGLEGGWNLYQYPLNPIEHIDPLGLALDLNYYSP
                     SDPIYKGSLNVREFPTGFTVGGHGSPTSMSDDRIKKGSDLTIKQLASDIRANPKYHEG
                     MPVVLFSCETGKGKNSFAQKLANELDATVIAPDEIIWIWPDGNYAIMGQTARITIGGK
                     DNGVFELVPDEKQPGDFHKFTPTGS"
BASE COUNT         1695 a         1895 c         2336 g         1322 t
ORIGIN      
        1 gcggttccgt gaagtgagct gcatctgcct gtctgggatt aacggcaggt cattgtaagt
       61 agtaattaac cacgttaaca aggagtttac aaatgccaac accgtgttat atctccatca
      121 ctggtcagac ccagggaaat attactgcag gtgcatttac cgctgattct gtcggcaaca
      181 tctatgtgca ggggcacgaa gacgagatgc tggtgcagga attcctgcat aacgttactg
      241 tcccgaccga tcctcagtcc ggtcagcctg cgggtcagcg tgctcacaag cctttcattt
      301 ttaccgtggc gctgaacaaa gcggttccgc tgatgtacaa cgcactggcc tccggcgaaa
      361 tgctgccaac cactgaactt cactggtggc gtacctctgt ggaaggcaaa caggagcatt
      421 atttcactac ccgcctgact gattcgacga ttgtcgatat gaaactgcat atgccgcatt
      481 gccaggaccc ggcgaaacgc gagttcaccc agttgctcga agtttcactg gcctaccgca
      541 aaattgaatg ggaacacgtc aaatctggca cctccggtgc cgacgactgg cgcgcaccgc
      601 tggaagcata agttaagcca acagcatccg gctatcaggc cggatgctgt ttttgtatta
      661 cgccgtagaa agaaaggttg tcagtaaggg gttacgttca cgggagatgg cggctgccgg
      721 aaacttcggc ggtagcgtgc ctgaacgcaa gcgtagcact gtactgtcac agccgcttta
      781 acagcgaact gtctgttttt ccttaacccc ggaggttgct atgtcaaccg gattacgttt
      841 cacgctggaa gtggacggcc tgccaccgga tgcttttgcg gtggtctcct ttcatctgaa
      901 ccagtcactc tcttcgcttt tttccctcgc tctctctctg gtcagccagc agtttctctc
      961 ccttgaattc cagcagatcc tcgacaaaat ggcctacctg acgatatggc agggcgatga
     1021 cgtacagcgc cgggtgaaag gtgtggtgac ctggtttgaa ctgggggaga acgacaaaaa
     1081 ccagaagctg tacagcatga aggtatgccc gccgctgtgg cgcacagggc tgcgccagaa
     1141 cttccgtatc ttccagaatg aggacatcgc aagcatcctc ggcacgatat tgcaggaaaa
     1201 cggggtgacc gagtggagcc cgctgttcag cgagccacat ccttcccgtg agttttgtgt
     1261 ccagtacggt gagactgatt acgatttcct gtgccggatg gcggcggagg aaggcatctt
     1321 cttttatgag gagcacgcgc aaaaaagtac cgaccagagc ctggtcctgt gcgataccgt
     1381 gctttatctg ccggagtcct ttgagatccc ctggaatccg aacacccgta ccgaggtaag
     1441 caccctctgc atcagccagt ttcgctacag cgcacaaatc cgcccttctt ccgtggtgac
     1501 caaagactac acctttaaac gccccggctg ggccggacgt tttgaccagg aaggccagca
     1561 ccaggattac cagcgcacac agtatgaagt gtatgactat cccggacgtt tcaagggcgc
     1621 ccacgggcag aactttgccc gctggcagat ggacggctgg cgaaacaacg cagaagtggc
     1681 gcgcggaaca agccgttcgc cggagatatg gccgggacgg cgaattgtgc tgacggggca
     1741 tccgcaggcg aacctgaacc gggaatggca ggtggtggca agtgaactgc acggtgagca
     1801 gccgcaggcg gtgccgggac gcaggggttc aggcaccacg ctggataacc attttgcggt
     1861 aataccggca gacagaacat ggcgaccaca gccgttgctg aaaccgctgg tggacggccc
     1921 gcagagcgcc gtcgtgacgg gaccggcagg cgaggaaatc ttctgtgatg aacatggccg
     1981 cgtgcgggtg aaatttaact gggaccgcta taacccgtca aaccaggaca gttcatgctg
     2041 gatccgtgtg gcacaggcgt gggcaggcac cgggtttggt aacctggcga taccgcgtgt
     2101 gggtcaggag gtcattgtgg acttcctcaa cggcgatccg gaccagccga tcattatggg
     2161 gcgtacctac caccaggaaa accgcacacc cggtagcctg ccggggacga agacgcagat
     2221 gaccattcgt tcgaaaacct ataagggcag cgggtttaat gaactgaagt ttgacgatgc
     2281 gacagggaaa gaacaggtct acatccacgc gcagaagaac atgaacaccg aggtgctgaa
     2341 taaccgcacc acggatgtga taaacaacca tgccgaaaaa ataggtaaca accaggcgat
     2401 caccgttacc aataaccaga tcctgaacat tggcgttaat cagatacaga cggttggtgt
     2461 caaccaggtg gaaacggtag gcagtaacca gattatcaaa gtgggatcaa accaggttga
     2521 aaaggtgggg atcattcgtg cgctgacggt gggtgtggcg tatcagacga cggtaggcgg
     2581 cattatgaat acctcggtgg cgttgttgca gtcctcacag gtagggctgc ataaatcact
     2641 gatggtggga atgggctaca gcgtcaatgt agggaataac gtcaccttct cggtgggcaa
     2701 gacgatgaag gaaaacaccg gacaaacagc agtttattct gccggtgagc atcttgaact
     2761 ctgctgtggt aaggcaaggc tggtgctgac gaaggacgga agcatatttc ttaatggtac
     2821 gcacattcat ctggaagggg agtcggatgt gaacggtgat gcgccggtga ttaactggaa
     2881 ctgtggtgcc acacaacctg taccggatgc gcctgtgccg aaagatttac cccccggaat
     2941 gccggatatg cggcaatttt gagtcaggtg caaaaaatca aaacggaact ggtgcagtga
     3001 ccagataaac catattaaaa gcagaaggaa ttgattcatg ggaggaaaac cggcggcgcg
     3061 gcagggtgac atgacccgca agggactgga tattgtgcag ggttcagcag gggtgctgat
     3121 aggtgcgccg acgggcgtgg cctgctcggt gtgtccggga gggattacct atgctaaccc
     3181 ggtgaacccg gtgctgggtg cgaaggtgct gccgggcgag acggaccttg cgctgcccgg
     3241 cccgctgccg tttattcttt cccgcgccta cagcagctac cggaccagaa cacccgcgcc
     3301 ggtgggggtg tttggtcccg gctggaaagc gcccttcgat atccgcttac agatacgcga
     3361 tgaaggcctg atactcaacg acaacggcgg caggagcatt cactttgagc cgctgtttcc
     3421 cggcgagata agctacagcc gcagcgagtc gttctggctg gcgcggggcg gggtggcgga
     3481 gcagcacagt tcgcagccgc taagcgcgct ctggcaggtg ctgccggaag atgttcgcct
     3541 gagtccgcat atgtacctgg cgacaaacag ccttcagggg ccgtggtgga tactcaactg
     3601 gccggagcgg gtgccggggg cggacgaggt gctgccgccg gagccgcccg cataccgggt
     3661 gctgacgggc gtggtggatg gcttcgggcg gacgctggcc tttcaccggg cggcggaggg
     3721 tgatgtggcg ggcgcggtga cgggggtgac ggacggcgcg ggccgccgtt ttcacctggt
     3781 gctgaccaca caggcacagc gggcggaagt attccgtaaa cagcgcgcca cgtctttatc
     3841 ttcccctgcc ggtccccgtt ctgcttcctc ttctctggtt ttccccgaca cgctgcccgc
     3901 cggtacagaa tacggtgccg ataacggtat ccggctggag gcggtatggc tgacacacga
     3961 cccggcatac ccggatgaac tgcccgccgc gccgctggcg cgctacacgt acacggccag
     4021 cggagaactg cgggcggtgt atgaccgcag cgggacgcag gtgcgcgggt ttgcttatga
     4081 tgcggagcac gccgggcgga tggtggcgca ccattatgcg ggtaggccgg agagccgcta
     4141 ccggtatgat gataccggcc gggtgacgga gctggtcaac ccggaggggc tggactaccg
     4201 ctttgagtac gggcaggacc gtgtgaccat cacggacagc ctgaaccggc gggaggtgct
     4261 gtacacggaa ggcgagggtg gcctgaaacg tgttgtgaag aaggaacatg cggacgggag
     4321 catcacccgc agcgagtatg atgaggcggg gaggctgaag gcacagacgg atgcggcggg
     4381 acggcggacg gagtacagcc tgcatatggc gtcgggtgcg gtgacagcgg tgacggggcc
     4441 ggacggcagg acggtgcggt atggctataa cagccagcgg caggtgacgt cagtgacgta
     4501 cccggacggg ctgcgcagca gccgggagta tgatgagaag ggaaggctgg cggcggagac
     4561 ctcgcgcagc ggagagacga cgcggtacag ctatgatgac ccggcgagtg agctgccgac
     4621 agggatacag gacgcgacgg gcagtacaaa acagatggca tggagccgtt acggtcagct
     4681 gctgaccttt acggactgct cggggtacac gacgcggtat gagtatgacc ggtacggtca
     4741 acaaatcgcc gttcaccggg aagaaggcat cagcacttac agcagttata acccgcgtgg
     4801 ccaactggtc agtcagaagg atgcgcaggg gcgtgaaacc cgttatgagt acagcgccgc
     4861 aggcgacctc accgctatcg ttgccccgga cggcagccgc agtgagatac agtatgatgc
     4921 gtggggaaag gccgtcagca ccacgcaggg cggtctgacg cgcagcatgg ggtatgacgc
     4981 tgccgggcgc atcaccgtgc tgaccaacga gaacggcagc cagtccacgt tccggtatga
     5041 cccggtggac aggctgactg aacagcgcgg ttttgacggc cggacgcaac gttaccacta
     5101 tgacctgacc ggaaaactca cgcagagtga agacgagggg cttgtcaccc tctggcacta
     5161 cgatgcgtcg gaccgcatca cgcaccggac ggtgaacggc gacccggcag agcagtggca
     5221 gtatgatgag cacgggtggc taaccaccct cagccatacc agtgaaggcc accgggtgtc
     5281 ggtccactac ggctatgacg ataaaggccg cctgacgggt gaacggcaga cggtggagaa
     5341 cccggagacg ggggagatgc tgtgggagca tgagacgggg cacgcgtaca gcgaacaggg
     5401 gctggcgacc cgtcaggagc cggacggtct gccgccggta gagtggctga cgtatggcag
     5461 cggttatctt gcggggatga agctgggcgg aacgccactg gtcgagtaca tgcgggaccg
     5521 gctgcaccgt gagacggccc gcagcttcgg cggggaggca tatgaacttg ccaccgcctg
     5581 gaataccagc ggccagctcc ggagcaggca cctgaacctt ccgcagcttg accgtgacta
     5641 cgactggaac gacaacggac agctaatccg catcagcggc ccgcaggaga gccgggagta
     5701 ccgttacagt gacacgggaa ggctgacggg cgtccacacc acggcagcga acctggatat
     5761 cgatatcccg tatgcaacgg acccggcagg aaaccggctg ccggacccgg aactgcatcc
     5821 ggacagcacg ctcacggcgt ggccggataa ccgcatcgcg gaagatgcgc actatatcta
     5881 tcgctacgat gaatacggca ggctggcgga gaagacggac cgtatcccgg aaggggttat
     5941 ccggatgcac gacgagcgca cccaccacta tcactacgac agccagcacc gcctggtgtt
     6001 ccacacgcgg atacagcacg gcgaaccaca ggtggagagc cggtacctct atgacccgct
     6061 gggccgccgg acgggaaaac gggtgtggcg gcgggagcgt gacctgacgg ggtggatgtc
     6121 gctgtcgcgt aaaccggagg agacctggta cgggtgggac ggtgacaggc tgaccactgt
     6181 acagacccaa cagacaagaa tccagacggt ataccagccg ggaagcttca cgccgctcct
     6241 gagaatcgaa acagagaatg gtgaacaggc gaaggcgcgg caccgtagcc tggcggaggt
     6301 gttgcaggag gacacgggtg tgacgctacc ggcggagctg gcggtgatgc tgggaaggct
     6361 ggagcgggag ctgcggcagg gcagcgtgag tgaagaaagc cagcagtggc ttgcgcagtg
     6421 cgggctgacg gcggagcaga tggccgcgca gctggaggcg gaatacatcc cggagaggaa
     6481 acttcatctt taccactgcg accaccgggg actgccgctg gcgctcatca gcccggaagg
     6541 ggaaacggcg tggcaggggg agtatgacga gtggggaaac ctgctgggcg aaaccagcgc
     6601 gcagcacctt caacagtcac tccgtctgcc ggggcagcag tatgatgagg agttggggct
     6661 gtactacaac cgcaaccggt actatgatcc gttgcagggg agatatatca cccaggaccc
     6721 gatagggctg gaggggggat ggaacctgta tcagtaccca ctcaatccta ttgaacatat
     6781 agatccgttg gggttagcac ttgatttgaa ttattattct ccgtcagatc ctatatataa
     6841 aggttctctt aatgtaaggg aatttccaac gggttttaca gttggaggtc atggttcgcc
     6901 tacatctatg agtgatgaca gaatcaaaaa aggaagtgat ctgacaataa aacaattagc
     6961 tagcgacata agagcgaatc ctaaatatca tgaaggtatg cctgtagtct tgttttcctg
     7021 cgaaacagga aaaggcaaaa attcatttgc acagaaacta gctaacgaac ttgatgctac
     7081 agtgattgct cctgatgaaa taatatggat ttggccggat ggaaactatg ctattatggg
     7141 gcaaacagct aggataacaa taggcggtaa ggacaacggg gtgtttgaat tggtgccaga
     7201 cgagaaacaa ccaggggact ttcataagtt tacacctaca ggaagcaa
//
LOCUS       DBFIFI010000214         7213 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.214, whole genome shotgun sequence.
ACCESSION   DBFIFI010000214 DBFIFI010000000
VERSION     DBFIFI010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7213
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.214"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(187..393)
                     /locus_tag="N7B39_004104"
     CDS             complement(187..393)
                     /locus_tag="N7B39_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU2423985.1"
                     /translation="MNQHNTRLIRLPEVLHRTGYGKAWIYHLINQNRFPSPVKIGARS
                     VAFIESEVDEWIQLTIENSRKHVA"
     gene            complement(493..1344)
                     /locus_tag="N7B39_004105"
     CDS             complement(493..1344)
                     /locus_tag="N7B39_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6387 family protein"
                     /protein_id="HGU2423986.1"
                     /translation="MKNWSVEHTREVKKWLDIDTYRGFEELPLIHLYHELLARTLFFK
                     PYHEEFEAEAVRLYIDRIFTGKPFLITEKHLGYLTREDTLYQPPHFFLTTTERLAQLS
                     IVGLRNSLFFWDGSDEYSVNREFLDSPVSEVLPKLFRDTVMVEIDLANGTDEEIAESL
                     KAALPQWRKVRGIEPDVTEAIRFGYGTIKKIINYRLLPMIDILVWSKLYKVRISEDRL
                     SRLLYTDDDDEINTRLNHQIRDTDKPLALKAASIPFIRQFNLFINKNSHLKKIRVSDV
                     MKIADSD"
     gene            complement(1771..6357)
                     /gene="ypjA"
                     /locus_tag="N7B39_004106"
     CDS             complement(1771..6357)
                     /gene="ypjA"
                     /locus_tag="N7B39_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin-like autotransporter YpjA/EhaD"
                     /protein_id="HGU2423987.1"
                     /translation="MNRTSPYYCRRSVLSLLISALIYAPPGMAAFTPDVIGVVNDETV
                     DGSQRVDERGTTNNTHIINHGQQNVYGGVSNGSLIESGGYQDVGRHNNYVGQSNNTTI
                     NGGRQSIHDGGISTGTIIESGNQDVYKGGISNGTTIKGGASRVEGGSANGTLIDGGSQ
                     IVKVQGHADGTTINKSGSQDVVQGSLATNTTINGGRQYVEQSTVETTTIKNGGEQRVY
                     ESRALDTTIEGGTQSLNSKSTAKNTQIYSGGTQIIDNTSSSDVIEVYSGGVLDVSGGT
                     ATNVTQHDGAILKTNTNGTTVSGTNSEGAFSIHNHVADNVLLENGGHLDINAYGSANK
                     TIIKDKGTMSVLTNAKADATRIDNGGVMDVAGNATNTIINGGTQNINNYGIATGTNIN
                     SGTQNIKSGGKADTTIISSGSRQVVEKDGTAIGSNISAGGSLIVYTGGIAHGVNQETG
                     SALVANTGAGTDIEGYNKLSHFTITGGEANYVVLENTGELTVVAKTSAKNTTIDAGGK
                     LIVQKEAKTDSTRLNNGGVLEVQDGGEAKHVEQQSGGALIASTTSGTLIEGTNSYGDA
                     FYIRNSEAKNVVLENAGSLTVVTGSRAVDTIINANGKMDVYGKDVGTVLNSAGTQTIY
                     ASATSDKANIKGGKQTVYGLATEANIESGEQIVDGGSTEKTHINGGTQTVQNYGKAIN
                     TDIVSGLQQIMANGTAEGSIINGGSQIVNEGGLAENSVLNDGGTLDVREKGSATGIQQ
                     SSQGALVATTRATRVTGTRADGVAFSIEQGAANNILLANGGVLTVESDTSSDKTQVNT
                     GGREIVKTKATATGTTLTGGEQIVEGVANETTINDGGIQTVSANGEAIKTTINEGGTL
                     TVNDNGKATDIVQNSGAALQTSTANGIEISGTHQYGTFSISGNLATNMLLENGGNLLV
                     LAGTEARDSTVGKGGAMQNQGQDSATKVNSGGQYTLGRSKDEFQALARAEDLQVAGGT
                     AIVYAGTLADASVSGATGSLSLMTPRDNVTPVKLEGAIRITDSATLTIGNGVDTTLAD
                     LTAASRGSVWLNSNNSCAGTSNCEYRVNSLLLNDGNVYLSAQTAAPATTNGIYNTLTT
                     NELSGSGNFYLHTNVAGSRGDQLVVNNNATGNFKIFVQDTGVSPQSDDAMTLVKTGGG
                     DASFSLGNTGGFVDLGTYEYVLKSDGNSNWNLTNDVKPNPDPNPNPNPNPKPDPKPDP
                     KPDPKPDPTPEPTPTPVPEKRITPSTAAVLNMAATLPLVFDAELNSIRERLNIMKASP
                     HNNNVWGATYNTRNNVTTDAGAGFEQTLTGMTVGIDSPNDIPEGIATLGAFMGYSHSH
                     IGFDRGGHGSVGSYSLGGYASWEHESGFYLDGVVKLNRFESNVAGKMSSGGAANGSYH
                     SNGLGGHIETGMRFTDGNWNLTPYASLTGFTADNPEYHLSNGMESKSVDTRSIYRELG
                     ATLSYNMRLGNGMEIEPWLKAAVRKEFVDDNRVKVNNDGNFVNDLSGRRGIYQAGIKA
                     SFSSTLSGHLGVGYSHGAGVESPWNAVAGVNWSF"
     gene            complement(6422..6541)
                     /locus_tag="N7B39_004107"
     CDS             complement(6422..6541)
                     /locus_tag="N7B39_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HGU2423988.1"
                     /translation="MPHSYAEYHHFIMMSLMSGEHNTLALSLQDESPGLVPVG"
     gene            complement(6765..6923)
                     /locus_tag="N7B39_004108"
                     /pseudo
     CDS             complement(6765..6923)
                     /locus_tag="N7B39_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT         1783 a         1808 c         1566 g         2056 t
ORIGIN      
        1 caaataaaca ggtacggcta gtgggctgta tccatacccg atattaagaa aaccatctct
       61 aagccttata gcaaaagctc tgccagaaaa aacgtaaaga attacgttaa atatagattt
      121 ttttaagatt taaactttac aaatctgaga attaatcctc ataaataaga agactgtcat
      181 tttatttcag gcaacatgct ttcttgaatt ttcgatagtt aattgaatcc attcgtctac
      241 ttcactttca atgaaagcaa cagagcgcgc accgatttta acgggggaag gaaaacgatt
      301 ttggttgatg aggtgataga tccaagcttt accgtatcca gttcgatgca gaacctctgg
      361 taaacgaata aggcgggtgt tgtgctgatt catagtcgtt tcctctgtgc cgttttgatg
      421 gaaatgacta tacaaatgaa tcagcaggaa aaagaccgaa gctgggaacg ccagagctaa
      481 tggaggttgg tcttagtcag aatcagcgat tttcatcacg tctgaaactc tgatcttttt
      541 caaatggctg ttcttgttga tgaacagatt aaactggcga ataaaaggta tactcgccgc
      601 cttcaaagca agaggtttgt ctgtatccct gatttggtgg ttcaggcgag tgttgatctc
      661 atcatcgtca tctgtataca acaggcgaga aaggcggtct tctgatatac gtactttata
      721 taattttgac cataccagaa tgtcgatcat gggaaggagt ctgtaattaa tgattttctt
      781 aatcgttcca taaccaaagc gaatagcttc cgttacatct ggttctatgc cacgcacttt
      841 acgccattga ggaagtgcag cctttaggga ttcagcgatc tcttcatctg tcccgtttgc
      901 taggtcgatt tcgaccatga cagtatccct gaacaatttt ggcaaaactt ccgatacagg
      961 gctatcgagg aattctctgt taacagaata ctcatcgctt ccatcccaaa aaaacagtga
     1021 gttccgtaat ccaactatgc tgagttgagc cagtctttcg gttgtcgtca aaaagaagtg
     1081 cgggggctgg taaagagtat cctctcttgt gagatagccg agatgctttt ctgtgatgag
     1141 aaagggcttt ccagtgaaga tcctgtcgat gtagagcctt acggcttctg cttcaaattc
     1201 ttcatgatat ggtttgaaga acaatgtcct tgccagcaat tcatggtaga ggtgtataag
     1261 cggaagctct tcgaatccgc gatatgtatc tatatccagc cacttcttca cctctctcgt
     1321 atgttcaact gaccagtttt tcataacgat ctccaacagg cattagactt aatgtatgta
     1381 gcaaacgtaa tcgacatgtt aaaggaagac caatgaaatg agttcagatg ccatcaggaa
     1441 gttgaaccaa aaggtttgat attttttgat acagttcgct aacggactat tttcaggggt
     1501 ttatgccgtt acgatttatg gttcctttat agaaccaaag gtaaagtcag aaacagcagt
     1561 tatctagaaa attcaatcaa atagatatct agttacaggg acgccagcca atcatgattg
     1621 gacggtgtaa ggacaacacc aacaaaaaca ggaagttaga agtctcagca ggacaccgac
     1681 cagacggtga ggagacataa aaggatacgc aaaggagccg cggctcctgg tgacatgaaa
     1741 gcccacagat gtgggctttt tcgttgatgg tcagaacgac cagttcacac cagctaccgc
     1801 gttccacggg gattccacac cggcaccatg gctatacccc accccaagat gcccgcttaa
     1861 cgtactgctg aatgaggctt taatacctgc ctggtatatt ccacgtctgc ccgacaaatc
     1921 attgacgaaa ttaccgtcat tattcacctt cacccggtta tcatcgacaa attctttgcg
     1981 cacagccgcc ttcagccacg gctcaatttc cataccgttc cccagacgca tgttgtaact
     2041 cagcgttgcg cccagttcac gatatatact gcgggtatcg actgatttcg attccatgcc
     2101 attggataaa tgatattcgg ggttatcagc ggtgaacccc gttaacgatg catacggcgt
     2161 caggttccag ttaccatcgg taaatcgcat cccggtttca atgtgaccgc ccagcccgtt
     2221 gctgtggtaa ctgccattgg cggctccacc gctgctcatt ttaccggcta cgttactttc
     2281 aaaacggttc agcttcacga caccgtccag atagaaacca ctttcatgtt cccaactggc
     2341 atagccgccc agagaataac tgcccacact gccatgtcct ccgcgatcaa aaccgatatg
     2401 tgaatgggaa taacccataa aagcgcccag cgtcgcaatc ccctcaggaa tatcattagg
     2461 gctgtcgatc cccactgtca ttccggtcag cgtctgctca aacccggccc ccgcatcggt
     2521 ggtgacatta ttacgggtgt tatacgtcgc cccccagaca ttattgttgt gtggactcgc
     2581 tttcattatg ttcaaccgct cgcgaatact gtttagctca gcatcaaata ccaacggtaa
     2641 tgttgctgcc atattgagta cggctgcggt agaaggcgtg atgcgtttct ccggaacggg
     2701 tgtcggcgtt ggctcgggag tcgggtctgg tttcggatcc ggttttgggt ctggttttgg
     2761 atccggcttc ggatttgggt tgggatttgg gttgggatcc gggttgggtt tgacatcatt
     2821 ggtcaggttc cagttgctgt tgccatcgct tttcaggaca tactcatagg tcccaagatc
     2881 aacgaaaccg ccagtattgc ccagcgaaaa cgaagcatcc cctccccctg ttttcaccag
     2941 cgtcatcgcg tcgtcagact gaggactgac gccggtatcc tgaacaaaga ttttaaaatt
     3001 accagtggcg ttgttgttga cgaccagttg atcgccccga gagcctgcaa cgttggtatg
     3061 caggtagaaa ttaccgctac cggaaagttc attggttgtc agcgtattgt atataccgtt
     3121 agttgtggca ggcgctgctg tttgtgctga taaataaaca ttaccgtcgt taagtagcaa
     3181 actgtttact ctatactcgc agttgctggt gcctgcacag gaattattgc tgttaagcca
     3241 gacactgccc cggctggcag ccgtcaggtc ggcaagcgtc gtatcaacgc cattgccgat
     3301 agttaatgtc gcgctatcgg taatccggat cgccccttcg agtttaactg gcgtaacatt
     3361 atcccgtggc gtcattaacg acaggcttcc tgtcgcgcca ctgaccgatg catccgccag
     3421 cgtacctgcg tagacgattg ctgtcccgcc agcaacctgg agatcttctg cccgggccag
     3481 agcctgaaac tcatcttttg accgcccaag ggtatattgc ccaccagagt taacctttgt
     3541 ggcggagtcc tgaccctggt tttgcattgc cccccccttg ccaaccgtgg agtcgcgagc
     3601 ttcggtacct gctaatacca ataaattacc gccattttcc agcaacatat tggtcgctaa
     3661 attgccggaa atggaaaaag tgccgtactg gtgagtaccg ctgatttcaa taccgttagc
     3721 cgtgctcgtc tggagagcgg caccgctgtt ctggacgata tctgtcgctt tgccattatc
     3781 gttgactgtc agcgtaccgc cttcattgat cgttgttttt attgcctctc cgttagctga
     3841 aactgtttgt attccgccgt cgttaattgt tgtctcattc gccacaccct cgacaatttg
     3901 ttcaccgccg gtgagcgtcg tgcctgtcgc agtggctttt gttttgacga tctcccgtcc
     3961 gcccgtattg acctgtgttt tgtcagaaga ggtgtctgac tccacggtta acactccgcc
     4021 atttgccagc aggatattgt tcgccgcacc ctgctcgatg ctgaacgcga cgccatccgc
     4081 gcgtgttcct gtgacccgcg tcgccctggt ggttgcaacc aacgcgccct ggctactctg
     4141 ctgtatcccc gttgcgctgc ctttctcccg cacatcgagt gtgccgccat cattaagcac
     4201 cgagttttca gccagaccgc cctcattaac tatctgtgaa ccgccattaa taatggaacc
     4261 ttccgctgtc ccgtttgcca taatttgttg taggccagag acgatatcgg tattgatcgc
     4321 cttaccataa ttctgaacgg tttgcgtgcc accattgatg tgtgttttct ctgttgaccc
     4381 accatcaaca atttgttcac cactttcgat atttgcttca gtggctaaac catataccgt
     4441 ttgcttgcca cctttgatat ttgctttatc agaagtggca ctggcatata ttgtttgggt
     4501 gccagcacta ttgagtacag tgccaacatc ttttccataa acatccattt tgccgttggc
     4561 attaataatc gtgtcaactg cccgggaacc agtgacgact gttaatgagc cagcgttttc
     4621 cagcactaca tttttagctt ctgaattcct gatgtagaaa gcatcaccat aactgttggt
     4681 tccttcgata agtgttccgg aggtcgtgga agcaattaat gcgccgccgg attgttgctc
     4741 aacatgctta gcctcaccac cgtcctgaac ctccagaacg ccgccattat taagtctggt
     4801 gctatctgtt ttagcctcct tctggacaat cagcttaccg ccagcatcaa tggtagtatt
     4861 tttcgccgag gttttagcca ctaccgtcag ttcgccggta ttttccagca caacataatt
     4921 agcctcccct ccggtaatag tgaagtgaga gagcttgttg tatccttcga tatcagtccc
     4981 tgcacccgtg ttggcaacta aagcactgcc cgtctcctgg ttaaccccat gtgcaatacc
     5041 gccggtatag acaatcagcg agcctccggc gctaatattg ctgccaattg ccgtaccatc
     5101 tttctcaaca acctgccggc tcccggagga tataattgtt gtgtcagctt tcccgccgct
     5161 tttgatattt tgcgttccgc tgttgatatt ggtgcctgtg gctatgccat aattattaat
     5221 attctgtgtg ccaccattaa ttatggtatt tgtcgcgttt cctgcaacat ccataacccc
     5281 gccattatct attcgggtcg catcagcttt agcattggtt aaaactgaca ttgttccttt
     5341 atctttaata atcgtcttgt ttgccgaacc atatgcgttt atgtctaaat gaccaccgtt
     5401 ttccagcaac acattgtctg ccacgtgatt gtggatggag aatgcacctt cactattcgt
     5461 accgctcacc gtcgtaccgt tagtgttagt ttttaaaatt gcaccatcgt gctgggtaac
     5521 atttgttgcc gtaccaccac taacatcaag cacgccaccg gaataaactt caataacatc
     5581 cgaggagctg gtgttatcaa taatttgcgt accaccagaa tagatctgag tattttttgc
     5641 cgttgactta ctattcagag actgagttcc gccttcaatc gtcgtgtcca gcgcacggct
     5701 ctcatatact ctttgctcac cgccattttt gatggtggtt gtttctactg tgctctgttc
     5761 aacatactgt cgaccaccat ttatggttgt gttcgttgcc agacttcctt gtactacgtc
     5821 ctgagagcca gacttattta tcgttgtacc atcagcatgc ccttgaactt ttactatctg
     5881 gctaccacca tcaatgagtg ttccattcgc actccctccc tctacgcgtg aagcaccgcc
     5941 cttaattgtc gttccattgc tgataccccc tttataaacg tcctgattgc cactctcgat
     6001 tatcgtacct gtggaaatac ccccgtcatg aattgactgt ctgcccccgt taatggtggt
     6061 attattagac tgccccacat aattgttatg ccttcctaca tcttgatatc caccagattc
     6121 aataagactt ccattagata ccccgccata aacattctgc tggccatggt tgataatatg
     6181 agtgttattt gttgtacctc gttcatctac tcgttggctg ccatctacag tctcatcgtt
     6241 taccacacca ataacatcag gagtgaaggc agccatcccg ggcggggcat atatcaaggc
     6301 agatatcaat aaggaaagta ctgagcgacg acaataatag ggactggtcc tgttcataaa
     6361 tttcatcctc tgaaaagtga atactgagta gcgtttaagc gaccttagct ttgctgcaac
     6421 atcagcccac aggcaccaga ccaggggatt catcctgaag agacagcgca agtgtattgt
     6481 gttcaccgct catcaaagac atcatgatga aatgatgata ttccgcataa gaatgaggca
     6541 ttttttaaac gcagtgcgct gaagtgtggt tggataaaaa agtcaatcca ttcaggaaat
     6601 acgggcgtat tcttttcttt cgacaatgag gccgttggca aaataaaatg atttacataa
     6661 tcgtttctga tgaatatctt ctgctcacat aaaaatcaca caataacttt gagatcgcag
     6721 attgttttac ttttacagca ttcgtccccc cattgttggg caaatataga ttgggccaga
     6781 gcacgaaagt taataccacg ttcgcacagc tcctccaaca gcacgacaag atgccacata
     6841 ctgcgcccca gtcggttcag tttacagact agcagtgctt tccctgccga tgatgtcctg
     6901 agcagttttt tcagtccgga cctcctgcca cggttttacg ttgaagcggt aaccctgagc
     6961 acgcagttct tcagtcaggc gtggtgcacc gtaacgctgt tattgctggg tgagatcaaa
     7021 aaactttcag gcagctaagg aaagttgaac cagacaatag atgaaatatt tcaaccaatt
     7081 acagcaccaa ttcagtcact gccagcccac caaataaatc aaggggttac atgaaaacgt
     7141 agcccctttt tctttggtag tgacactaaa atggatgtag tgtgaagaat aatcccgttt
     7201 actcaatcaa taa
//
LOCUS       DBFIFI010000215         7186 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.215, whole genome shotgun sequence.
ACCESSION   DBFIFI010000215 DBFIFI010000000
VERSION     DBFIFI010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7186
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.215"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..4494)
                     /locus_tag="N7B39_004109"
     CDS             complement(<1..4494)
                     /locus_tag="N7B39_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HGU2423989.1"
                     /translation="MSEGPGGPQGATAGGTLAMRMLSQRAMAASQMKRAANDKAIAQM
                     LASKKSGPPAARLGDEIQHKSFLGALAGAVLGAIVTIAEGCLIMAACATGPYALVLVP
                     ALMYASYKASDYVEEKQNQLESWINSFCDTDGAINTGSKNVKINGKPAARAAVTLPPP
                     PPPGAIPEVPQGEPSWGDIATDLLESAAEKAVPLAKAWGNAVITLTDSNAGFMDRVSA
                     GASLLFPAGPVLMEFATMVGGRGEIKKDVDFPEAGEDTALCDKENKPPRIAQGSSNVF
                     INNQPAARKGDKLECSAAIVEGSPDVFIGGEQVTYLDIQPEFPPWQRMILGGITIASY
                     LLPPAGLLGKLGNLAKLGKLGNLLGKSGKLLGAKLGALLGKTGNSLKSIANKVIRWVT
                     DPVDPVTGAYCDERTDFTLGQTLPLSFTRFHSSVLPLHGLTGVGWSDSWSEYAWVREQ
                     GNRVDVISLGATLNFAFDGESDTAVNPYHAQYILRRRDDYLELFDRDALSSRFFYDAF
                     PGMRLRHPVTDDTSDDRLAHSPADRMYMLGGMSDTASNRITFERDTQYRITGVSHTDG
                     IRLKLTYHASGYLKAIHRTDNGIQTLATYEQDARLDYHLFYEYDAADRIIRWSDNDQT
                     WSRFTYDAQGRCVTVTGAEGYYNATLDYGDGCTTVTDGKGIHRYYYDPDGNILREEAP
                     DGSTTTYEWDEFHHLLARHSPAGRVEKFEYNAAHGQLSRYTAADGADWQYCYDERGLL
                     SNITAPAGQTWTQQCDERGLPVSLVSPQGEETRLAYTPQGLLSGIFRQDERRLGIEYD
                     HHNWPETLTDVMGREHHTEYSGHDLPVKMRGPGGQSVRLQWQQHHKLSGLERAGTGAE
                     GFRYDRHGNLLAYTDGNGVVWTMEYGPFDLPVARTDGEGHRWQYRYDKDTLQLTEVIN
                     PQGESYLYILDNCGRVTEERDWGGVVCRYRYDADGLCTARVNGLEETILYSRDAAGRL
                     AEVITPEGKTQYAYDKSGRLTGIFSPDGTSQRTGYDERGRVNVTTQGRRAIEYHYPDE
                     HTVIRCILPPEDERDRHPDGSLLKTTYRYNAAGELTEVILPGDETLTFSRDEAGREVL
                     RHSNRGFACEQGWNAAGQPVSQRAGLFPAEATWGGLLPSLLREYRYDSAGNVSGVTSR
                     EDYGRETHREYRLDRNGQVTAVTASGTGLGYGEGDETYGYDSCGYLKAQSAGRHRISG
                     ETDQYAAGHRLKQAGNTQYDYDAAGRMVSRTKHRDGYRPETERFRWDSRDQLTGYRSA
                     QGEQWEYRHDASGRRTEKRCDRKKIRFTYLWDGDSIAEIREYRDDKLYSVRHLVFNGF
                     ELISQQFSRVRQPHPSVAPQWVTRTNHAVSDLTGRPLMLFNSEGKTVWRPGQTSLWGL
                     ALSLPADTDYPAPRGERDPEADPGLLYAGQWQDAESGLCYNRFRYYEPETGMYLVSDP
                     LGLQGGEQTYRYVPNPCGYIDPLGLAICQLARWTKWGSEQSNISDVLNSLGNRALKYA
                     NGDW"
     gene            complement(4514..4975)
                     /locus_tag="N7B39_004110"
     CDS             complement(4514..4975)
                     /locus_tag="N7B39_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HGU2423990.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITRDPVSQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            complement(5003..6904)
                     /locus_tag="N7B39_004111"
     CDS             complement(5003..6904)
                     /locus_tag="N7B39_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HGU2423991.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLTQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTILQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MEGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     RGAGTALENHFAVIPADRTWRPQPRLKPLVDGPQSAVVTGPEGEEIFCDEHGRVRVKF
                     NWDRYNPADQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGREQVYIHAQKNMDTEVLND
                     RTTTVKHDHRETVKNDQTVTIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGTIAGS
                     VHVDAVNNGGEGNGIQAYTAIKEIMLAVEESKIALTPDGIQLQVGESTVIRLSKDGIT
                     IVGGSVFIN"
BASE COUNT         1345 a         2192 c         1950 g         1699 t
ORIGIN      
        1 ccaatcacca ttagcatatt taagtgctct attccctaat gagttcaaaa catcagatat
       61 gttgctttgc tcactccccc atttcgtcca gcgggctaac tgacatatag ccagccccaa
      121 aggatcgata tacccacaag gattcggcac ataccgataa gtctgctccc ctccctgcaa
      181 ccccagcgga tcactcacca ggtacatccc ggtttccggc tcgtagtacc ggaaccggtt
      241 atagcacagc cccgattctg catcctgcca ctgtcccgca tacagcaggc cggggtccgc
      301 ttccgggtcc cgctccccgc gcggggccgg gtagtctgtg tctgcgggca ggctgagtgc
      361 cagcccccac aggctggtct gccccggccg ccagacggtt ttaccttcac tgttaaagag
      421 catcagcggg cggcccgtca ggtcgctcac cgcatgattc gttcgcgtca cccactgcgg
      481 ggccacggac ggatgcggct gtcgtacccg gctgaactgc tggcttatca gctcaaagcc
      541 gttaaacacc aggtgcctta cgctgtacag tttatcatcg cggtattccc ggatttccgc
      601 aatactgtcg ccgtcccaca ggtacgtaaa acggattttc ttccggtcgc agcgtttttc
      661 cgtccgtctg ccgctggcgt cgtggcggta ttcccactgc tccccctgtg cgctgcgata
      721 cccggtcagc tggtcccggc tgtcccaccg gaaccgctct gtttctgggc ggtagccgtc
      781 acggtgtttt gtgcggctga ccatccggcc tgcggcgtca tagtcatact gtgtgtttcc
      841 ggcctgtttc agccggtggc ctgcggcata ctggtcagtc tctccgctta tccggtgtct
      901 gcccgcagac tgcgccttca ggtagccgca gctgtcatag ccataagtct cgtcgccttc
      961 gccatagccc agcccggtgc ctgaggctgt caccgccgtg acctggccgt tccggtcaag
     1021 ccggtactcc cggtgtgttt cccgtccgta atcttcccgg ctggtgacgc ctgatacgtt
     1081 acccgcgctg tcgtaacggt attcccgtag cagtgaaggg agcagtccgc cccatgtggc
     1141 ttccgccggg aaaagtccgg cgcgctggct gacaggctga ccggctgcat tccagccctg
     1201 ttcacaggca aaaccccggt tactgtgccg gagcacttca cgtcccgcct catcacggct
     1261 gaacgtcagc gtctcatccc ccggcaggat aacctccgtc agttctccgg cggcgttgta
     1321 gcggtatgtg gttttcagca gggatccgtc ggggtgtctg tcgcgttcat cttccggtgg
     1381 caggatacag cggatgacgg tgtgttcgtc ggggtagtgg tattcaatgg cccgtcggcc
     1441 ctgagtggtg acattcaccc gcccgcgttc atcatagccg gtgcgctgtg atgtgccgtc
     1501 cgggctgaag atacccgtca gcctgccgga tttgtcatac gcatactgcg ttttgccttc
     1561 cggagtgatg acttctgcca ggcggcctgc ggcatcccgg ctgtagagga tggtttcctc
     1621 caggccgttg accctggcgg tacacaggcc atcagcgtca taacggtaac gacagaccac
     1681 gccgccccag tcacgttctt ccgtcacccg gccacagttg tccagaatat aaagataaga
     1741 ctcgccctgc gggttaatga cttctgtcag ttgcagcgtg tctttatcgt agcggtactg
     1801 ccagcggtgg ccttcaccgt ccgttcgcgc caccggcaaa tcaaacgggc cgtactccat
     1861 tgtccagaca acgccgttac cgtccgtgta cgccagcagg ttgccgtggc ggtcgtagcg
     1921 gaatccttcc gcgccggttc ctgcccgctc aaggccactc agtttatggt gctgctgcca
     1981 ctgcaaccgc actgactgac cgccggggcc gcgcatcttc accggcaggt cgtgaccgct
     2041 gtattcggtg tggtgttcgc ggcccatcac gtcggtgagt gtttccggcc agttgtggtg
     2101 gtcgtactct atgcccagac gccgttcatc ctggcggaat atccccgaca gcagcccctg
     2161 aggggtgtac gccagccggg tctcttcgcc ctgtggcgat accagactca ccggcaggcc
     2221 gcgttcatca cactgctgcg tccacgtctg cccggcaggg gcggtgatgt tgctgagcag
     2281 gccgcgctca tcatagcagt actgccaatc cgcgccgtct gccgccgtgt aacggcttaa
     2341 ctgaccgtgt gcggcgttgt attcaaactt ctccacccgc ccggcagggg agtggcgggc
     2401 cagcaggtga tggaattcat cccattcata cgtggtggtg ctgccgtccg gcgcttcttc
     2461 ccgcagaata ttgccgtcag gatcatagta ataacggtga atgcccttgc cgtccgtcac
     2521 ggtggtgcag ccgtcaccat agtccagcgt ggcgttgtaa tagccctccg ccccggtgac
     2581 ggtcacgcac cggccctgtg catcgtaggt gaaacggctc cacgtctggt cgttatcgga
     2641 ccagcggatg atccggtccg cagcgtcgta ctcataaaac aggtggtagt ccagccgcgc
     2701 atcctgttcg taggtcgcca gcgtctgtat gccgttatcc gtgcggtgaa tggctttcag
     2761 gtagccgctg gcgtggtacg tcagtttaag ccggatcccg tcggtgtgac tgacacccgt
     2821 gatccggtac tgggtgtcgc gctcaaacgt gatgcggttg ctggcggtgt cgctcatccc
     2881 gcccagcatg tacatccggt ctgcggggct gtgtgccagg cggtcatcgc tggtatcgtc
     2941 agtcaccggg tggcgcagac gcattcccgg aaaggcgtca taaaagaagc ggctgctcag
     3001 tgcatccctg tcgaacagct ccagataatc atcacggcgg cgcagaatgt actgggcgtg
     3061 atacgggtta accgccgtat cactttcacc gtcgaaggcg aagttcagcg tggctcccag
     3121 gctgatgaca tccacccggt ttccctgttc acgcacccag gcgtattcgc tccaggagtc
     3181 gctccagccc acgcccgtca ggccatgcag cggcagtaca gaactgtgga aacgggtgaa
     3241 ggagaggggg agtgtctggc ccagcgtaaa gtcggtacgt tcgtcgcagt atgcgccggt
     3301 taccggatcg acaggatctg ttacccatct gatgacttta ttggcaatac tttttaacga
     3361 attacctgtt ttccccagca acgcgccgag ctttgcgccc agcagcttac cgcttttccc
     3421 cagcaggttt cccagtttgc ccagtttcgc cagattcccc agttttccca gcagtcctgc
     3481 tggcggcaga agatagctgg ctatcgttat tcctcccagg atcattctct gccatggcgg
     3541 gaactccggc tggatatcca gataggtgac ctgctcaccc ccaataaaga cgtccggcga
     3601 accttccacg attgccgcgc tgcactccag tttgtcgccc ttgcgcgcgg caggctgatt
     3661 gttgataaag acgttgctgc tgccctgggc tatcctcggt ggtttgttct ccttgtcgca
     3721 gagcgccgtg tcctcaccgg cttccgggaa atccacatct tttttgattt cgccacgccc
     3781 gcccaccatg gtggcaaact ccattaatac cggaccggcg ggaaacagaa gcgatgcgcc
     3841 ggcgctgacg cgatccataa aaccggcatt gctgtccgtc agggtgataa cagcgttccc
     3901 ccaggccttc gccagtggta ctgctttttc cgctgccgat tcaagcaggt cagtggcaat
     3961 atcaccccat gagggttccc cctgtgggac ttcaggtatt gctccaggtg ggggaggagg
     4021 gggaagggtg acggctgcac gggctgctgg ctttccgtta atttttacat ttttagaacc
     4081 ggtattgatg gcgccgtccg tgtcacaaaa gctgtttatc catgattcaa gctggttctg
     4141 tttctcctcc acataatcac tcgccttata gctggcatac atcagcgcag gcaccagaac
     4201 cagcgcataa gggccggtgg cacaggcggc cataatcagg caaccttctg ctatggtcac
     4261 tatcgccccc agcacggccc ctgccagcgc ccccaaaaaa ctcttgtgct gaatttcatc
     4321 gcccagcctg gcggcggggg ggccagactt ttttgatgcc aacatctgtg caatggcttt
     4381 gtcgttggct gcccgtttca tctggctggc ggccatcgcc cgctgcgaca gcatccgcat
     4441 tgccagcgta ccgcctgcgg tcgctccctg tggcccgcct ggtccttcac tcatgtttta
     4501 tccctcgttt atgtcagcgc ggctggtcct tttggatgtc attctccggt ggcaacgggg
     4561 tgaagctctt tatcacctcc agcagcatcg tcttttgact gttcttcagg gataaggagg
     4621 tgctgaaagt gaaggtcagc agcgtatctc cacgccgctg gaatacggct atctggctaa
     4681 tccgcccctg attcccttca tcttccggac cgttccagga ataatccagc agccaggccg
     4741 gggcctctgc cagagtgatt tcccggcagg catgttcggt gtacccatca agtttgccct
     4801 ggtacagcgc cagcgtttcc tggtacagct ctggtgtcag ggttccctgt gaaacggggt
     4861 cgcgggtaat gaccaggttg atgccactgt tatccgtgga taccaggacc tgcatcgact
     4921 gatcctgcca ttgcgatggc agttgaatat gaccttcatt aaaagtgaac tgcataaaat
     4981 actccctgtt aatctgagtg aatcagttga tgaaaacaga accgcccacg atggtgatgc
     5041 catccttcga cagcctgatt accgttgatt ccccgacctg tagctgaatg ccatccggcg
     5101 tcagcgcaat tttgctttcc tccacggcca gcataatttc cttaattgcc gtataagcct
     5161 gtataccgtt gccttcgcca ccattgttta cagcgtcaac atgcaccgaa ccggcaatcg
     5221 tgcctctgtc ctgaaagaca tcctcagata aagacccttt cagtactccg gtgatcttgt
     5281 ggcctttttc caccgtaaga aggcggttac cttcctggat cgtgaccgtc tggtcatttt
     5341 ttacggtttc gcgatgatcg tgttttacgg tggtggtacg gtcgttgagc acttcggtat
     5401 ccatgttctt ctgcgcgtgg atgtagacct gttctctccc tgtcgcatca tcaaacttca
     5461 gctcattaaa cccgctgccc atatacgttt tggaacggat ggtcatctgc gtcttcgtcc
     5521 ccggcaggct gccgggggtg cggttttcct ggtggtaggt gcgccccata atgatcggct
     5581 ggtccggatc gccgttgagg aagtccacta tcacctcctg acccacacgc ggtatcgcca
     5641 ggttgccaaa accggtgcct gcccacgcct gtgccacacg gatccagcac gagctgtcct
     5701 ggtctgccgg gttataacgg tcccagttga atttcacccg cactcggcca tgttcatcac
     5761 agaagatttc ctcaccctcc ggccccgtca cgacggcgct ctgcggaccg tccaccagcg
     5821 gttttagccg cggctggggt cgccatgtcc ggtctgccgg gatcaccgca aaatggttct
     5881 ccagcgccgt ccctgctccc cgccttcccg gcaccgcctg cggctgctcg ccgtgcagtt
     5941 cacttgccac cacctgccat tcccggttca ggttcgcctg cggatgcccc gtcagcacaa
     6001 ttcgccgtcc cggccatatc tccggcgagc gactcattcc ccgcgcggtt tctgcattgt
     6061 ttcgccagcc ctccatctgc cagcgggcaa agttctgccc gtgggcgccc ttgaaacgtc
     6121 cggggtagtc atacacctca tactgcgtgc gctggtaatc ctggtgctgg ccttcctgat
     6181 caaaacgtcc tgcccagccg gggcgtttaa aggtgtagtc tttggtcacc acggaagaag
     6241 ggcggatttg tgcgctgtag cgaaactggc tgatgcagag ggtgctcacc tcggtacggg
     6301 tgttcgggtt ccaggggatt tcaaaagatt cgggcagatg gcggaccgtg tcgcacagca
     6361 ccaggctctg gtcggtactt ttgtaagcat gctcctcata aaagaagatg ccttcctccg
     6421 ccgccatccg gcacaggaaa tcgtaatcag tctcaccgta ctggacacaa aactcacggg
     6481 aaggatgcgg ttcactgaac agcggactcc actcggtcac cccgttttcc tgcaatatcg
     6541 tgccgaggat gcttttgatg tcctcgttct ggaagatacg gaagttctgg cgcagaccgg
     6601 cacgccacag cggcgggtgc accttcatgc tgtacagcat ctggtttttg tcgttctccc
     6661 ccagttcaaa ccaggtcacc acacctttca cccggcgctg tacatcatca ccctgccata
     6721 ttgtcaggta ggccattttg tccagcacct gcgcaaattc aagggagaga aactgctggc
     6781 tgaccaagga gagatcgagg gaaaagagcg aagagagtga ctgggtgaga tgaaaggaaa
     6841 ccaccgcaaa agcgtccggt ggcaggccgt ccacttccag tgtgaaacgt aatccggttg
     6901 acatagcaac ctccggtaaa atgaataata atccgcaccg tacaactgcg gataatgaga
     6961 taagcgggac gacttaagac gttattaggg aaggaacaaa aaatgctatg attatgtgag
     7021 gattttgttt aaatattact ttacagaaac gggtgtctgt tcagtcaaaa aaataatcac
     7081 aagctcaact tattgtttcc tgacgttatt caggacaggt gcgcacatca cagcgagtaa
     7141 caaaaacgca aagttaaacc aatattcata atatgttttt attcac
//
LOCUS       DBFIFI010000216         7169 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.216, whole genome shotgun sequence.
ACCESSION   DBFIFI010000216 DBFIFI010000000
VERSION     DBFIFI010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7169
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.216"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..493)
                     /locus_tag="N7B39_004112"
     CDS             complement(<1..493)
                     /locus_tag="N7B39_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HGU2423992.1"
                     /translation="MSKIDYQELREKAEKATKGSYIVGHTSVNQHGNLTGVFVCQKWK
                     GEPGGVIAECHVNCLVETDAQAYANAEFIAAFNPNVALALLDERERNQQYIKRRDQEN
                     EDIALTVGKLRVELEAAKSKLNEQREYYEGVISDGSKHIAELEKQCAEWERKALSNFE
                     ECAA"
     gene            complement(490..729)
                     /locus_tag="N7B39_004113"
     CDS             complement(490..729)
                     /locus_tag="N7B39_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2423993.1"
                     /translation="MFKQNEKSIAQIAEYIPRTCRGMQLQEAKARLEKKIALYIDDGC
                     DAAVLNAAFAPALNCHTRESFFSCIAAQLHKGGNQ"
     gene            complement(722..925)
                     /locus_tag="N7B39_004114"
     CDS             complement(722..925)
                     /locus_tag="N7B39_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019705292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2423994.1"
                     /translation="MTIVKTHTGIVITKDGPQVKKLHQTERMWVVGKNEFYHKETGRR
                     HFAENTRRRLLIDTIKPVEVKHV"
     gene            complement(922..1800)
                     /locus_tag="N7B39_004115"
     CDS             complement(922..1800)
                     /locus_tag="N7B39_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HGU2423995.1"
                     /translation="MSTHHVVSFSGGRTSAYLVHLMEQRRKAGENVHYILMDTGCEHP
                     LTYRFIREVVKFWDIPLTVLQVDINPELGQPNGYTEWEPKDIQTRMPVLKPFMDMVKK
                     YGTPYIGGAFCTDRLKLTPFIKYCDDHFGQGNYITWLGIRADEPRRLKPKPGVRYLAE
                     LSDFDKSDVIRWWRKQPFDLQIPEHLGNCVFCIKKSTQKLGLACKDEPGLMRVFNELV
                     TGKHVRDGHRRTGKDVMYRGHLTLDGIARMYADSDYSNLYQAMVLAKRFDTGSCSESC
                     EIWGDQLELKFEEVVA"
     gene            complement(1908..2351)
                     /locus_tag="N7B39_004116"
     CDS             complement(1908..2351)
                     /locus_tag="N7B39_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2423996.1"
                     /translation="MQFKDLPEDIQKIAADTLKAHLSVLDLTKEPKANLENISRNVRD
                     VFVGLYAYDNEKHEEHIQNGCINKFQQNIKLPVADKTEQKTDVLVFWNTVEAIARSTM
                     LEFNNINHSGIDTHQSSHREGFSILLTLLQEQGEYPQPTSNDTEQ"
     gene            complement(2428..3249)
                     /locus_tag="N7B39_004117"
     CDS             complement(2428..3249)
                     /locus_tag="N7B39_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HGU2423997.1"
                     /translation="MSQLDSNAIKEIVKLTTTAFSGENLPLTECPVALLPDNVNIESL
                     ERFMTERFRFRGVMTTTSIDDFVEYSKGYADEHSRCFINAETMKAVTVFNIGTLEQPG
                     HADNKALLELKATSPYRALRDVDGKKQLQKSLAEWLEDWADFLTAYDSDGNVLDIKQA
                     ISAVRRLTIDAKRSAEYEEQNFSGSRSVMESVEAKSKEIMPATFRFECIPYEGLGNRE
                     FTLRLSILTSEQPVLVLRIVRVEAAEEEIAKEFRDLLKERFEEEDILTFIGTFSV"
     gene            complement(3313..3660)
                     /locus_tag="N7B39_004118"
     CDS             complement(3313..3660)
                     /locus_tag="N7B39_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2423998.1"
                     /translation="MNQHQTDVNVFINDLDGGVFVNKLGAVLSEVAFGVNSTNKKGKV
                     CVEFELSSLDENRVSVSHKLKFTRPTMRGSKSEEDTTNTPMFVNKGGELTLFQKDQGQ
                     LFDKQGQHDAVLR"
     gene            complement(3735..4322)
                     /locus_tag="N7B39_004119"
     CDS             complement(3735..4322)
                     /locus_tag="N7B39_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HGU2423999.1"
                     /translation="MDEVIFTYNEESALTAGQGGFITETGAHIINITEAELKQSEKGA
                     RFIEFSGESDDGRKIQYLSVCVQKNDGTENKFGASIIHAMMGCTGIGQLTQHMVSVSK
                     YVAPEFHGKKIGLVLQKVLTTNRKTGADGYQMEIRIPFIAETGQTLKEKAEGKKPETV
                     ANMVSTLKDKDNRSKNVNPNHTDDPGYWQYGSDSF"
     gene            complement(4322..5011)
                     /locus_tag="N7B39_004120"
     CDS             complement(4322..5011)
                     /locus_tag="N7B39_004120"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HGU2424000.1"
                     /translation="MTPEIILQRTGVDITSLDQGDDGWHKLRLGVITASEVHNVIAKP
                     RSGSKWPDTKISYFHTLLAEVCTGVAPEVNAKSLAWGKQYEDDARALFEFIADVTVSE
                     TPIIFRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFDAIKPAYMAQVQF
                     SMWVTDKDAWYFANYDPRMKREGLHYVVVERDEKYMASFDEMVPEFIDKMDEALAEIG
                     FVFGEQWGVNN"
     gene            complement(5008..5958)
                     /locus_tag="N7B39_004121"
     CDS             complement(5008..5958)
                     /locus_tag="N7B39_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HGU2424001.1"
                     /translation="MFDIVEFVKQQERFFCEALTEPTLTWAKESQFAIQQFQKNAFLA
                     DTARANLPSAQNAIINVAAIGITLNPASKLAYLVPRKKAVCLDISYMGLLHLAQVTGA
                     IQWGQCKLVYEKDIYESNGIDCAPTHKYNPFVDRGARIGGYCVVKTSEGDYLTEEMSN
                     REIEVIRACSKAGNNGGSSPWDSFPDEMARKAIVKRASKYWPRRDRLDTAIDYLNTQA
                     GEGIILNADHIPERDVTPASDEIINEITQAITEINKTWDDLLPLCSKTFRRTIASHEY
                     LSQEEAVKTLDFVKKKAARNKATAEAKIHATTENNSEAVS"
     gene            complement(5975..6256)
                     /gene="gamL"
                     /locus_tag="N7B39_004122"
     CDS             complement(5975..6256)
                     /gene="gamL"
                     /locus_tag="N7B39_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000995345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host nuclease inhibitor GamL"
                     /protein_id="HGU2424002.1"
                     /translation="MNAYLTCDRIEERRWINQQIQDEKDKWIDDRAQELISMFPDKPL
                     LMSSLFLPKEAQLALTGEKAEEAYNDYISAIAYARAEEEWERKFSPCPF"
     gene            complement(6277..6558)
                     /locus_tag="N7B39_004123"
     CDS             complement(6277..6558)
                     /locus_tag="N7B39_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HGU2424003.1"
                     /translation="MLNQKININVKSVVTPAGTIMGEVFMDDKIIAYFVILSDEAISV
                     IDTEGNVMFIAEHPEDIALQAAAYFFAKEQEEECNCPVCQLSRQINLMH"
     gene            complement(6853..>7169)
                     /locus_tag="N7B39_004124"
     CDS             complement(6853..>7169)
                     /locus_tag="N7B39_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424004.1"
                     /translation="EQLQLPESTLSINYPLSWFSDNHPYATMNYVDRKVISLDASVLF
                     DMPSPTMRILNELHSKGYNVDAAVAEFNAFKHLTEEMRRQLLDISRTSEKSSRFGFNV
                     NL"
BASE COUNT         1659 a         1698 c         1652 g         2160 t
ORIGIN      
        1 tcgcagcaca ctcttcaaag ttgcttaatg cttttcgctc ccattcggcg cattgttttt
       61 ccagttctgc tatgtgctta cttccatccg agataacgcc ctcgtaatac tcacgctgct
      121 cgttgagttt tgattttgct gcttcaagct caacgcgcag cttccctacc gttagcgcaa
      181 tatcctcgtt ctcctggtcg cggcgtttga tgtattgctg gtttctttcc cgttcatcca
      241 gcagtgccag cgcaacattt ggattaaagg cagcaataaa ttcagcgttt gcgtaagcct
      301 gagcatctgt ttcaaccagg cagttaacat gacattctgc aatcacgcca ccgggttctc
      361 ctttccattt ttggcaaaca aaaactcctg ttaaattgcc gtgttggtta acagatgtat
      421 gccctacgat gtagcttcct ttagttgctt tctctgcctt ttcacgcagt tcctgatagt
      481 caatcttgct cactggttgc ctcctttgtg gagctgcgct gcgatgcacg aaaaaaaaga
      541 ttcccgcgta tgacagttaa gagctggtgc gaaagccgcg ttaagaacgg cagcatcaca
      601 gccgtcatcg atatagagcg caattttttt ctccaggcgc gctttggctt cctgcaactg
      661 cataccccgg cacgtacgcg ggatatactc agcaatttga gcgatagatt tttcgttctg
      721 tttaaacatg cttcacctcg acaggcttga ttgtgtcgat cagcagtctg cggcgcgtat
      781 tttctgcaaa gtggcggcgt ccggtttctt tgtggtaaaa ctcgtttttg ccgacgaccc
      841 acatccgctc tgtctggtga agttttttta cctgcgggcc gtctttggtg atcacaatgc
      901 cggtatgggt ttttacgatt gtcatgccac tacctcttcg aatttcaact ccaattggtc
      961 accccagatt tcacatgatt cggaacacga accagtatca aaccgcttgg ccagcaccat
     1021 cgcttgatac aaattgctgt agtcgctgtc ggcatacatc ctggcaatcc cgtcaagcgt
     1081 caggtgacca cggtacataa cgtctttacc tgttctgcga tgaccatccc tgacgtgttt
     1141 gcctgtaacc agttcattaa aaacccgcat cagaccaggt tcgtctttac atgcaagccc
     1201 cagcttttgc gttgactttt tgatgcagaa aacacagttc ccgaggtgct ccgggatttg
     1261 caaatcaaaa ggttgttttc gccaccaccg gataacatcc gacttatcaa aatctgacag
     1321 ttcggcaaga taccggacgc ccggtttcgg tttcagccta cggggttcgt ctgcacgaat
     1381 acccagccac gtgatgtagt tcccttgtcc gaaatggtca tcgcagtatt ttatgaaggg
     1441 ggtgagtttt aatctgtcag tgcagaacgc gccgccgatg tatggcgtgc catatttttt
     1501 taccatgtcc ataaacggtt taagcaccgg cattcgcgtc tgaatatcct ttggttccca
     1561 ttccgtataa ccatttggct gcccaagctc tggatttata tcgacctgca acacggttag
     1621 cggaatatcc cagaacttca caacctcccg aataaaccgg tacgtcagcg ggtgttcaca
     1681 tcccgtatcc atgagtatgt aatgaacgtt ttcaccagct tttctgcgtt gctccataag
     1741 gtggacgagg tacgccgatg tccgaccgcc tgaaaaactc acaacatgat gagtgctcat
     1801 ttcttcgtct cgttacgggg agggggttaa ataggtggta agctggaatt gccggatgtt
     1861 tacccgtgtc cggggtacgg cctcacgttg cagcgtacga gaatggacta ttgctcagtg
     1921 tcgtttgatg tcggctgagg atattcgcct tgctcttgta acagagttag aagaatggaa
     1981 aacccctcac gatgtgatga ttgatgagtg tcgatcccgg aatgattgat gttgttaaat
     2041 tccaacatcg tgctcctggc gatagcctca acggtattcc agaatactaa tacatctgtt
     2101 ttctgctctg ttttatcagc aacaggaagt ttgatatttt gttgaaattt attaatgcaa
     2161 ccattttgaa tatgttcctc gtgcttttca ttgtcataag catacagccc gacaaaaaca
     2221 tctcgcacgt tacgggatat attttccaga ttcgcctttg gttcctttgt taaatcaagc
     2281 accgataaat gggctttaag cgtatccgca gctattttct gaatatcctc tggtaaatct
     2341 ttaaattgca tagtataccc tccaagaact gtgtattttc tcgtgaaata tgagcgatat
     2401 gtcgtgacgt ccctgtcaca gatttcatca cactgagaac gtgccgatga aggttaagat
     2461 atcttcttct tcgaaacgct ctttcagcag gtcacggaac tctttggcaa tttcttcttc
     2521 cgcagcttca acacgcacga tacgtagcac cagaacaggc tgttcgcttg tcagaatgct
     2581 taatcgcagc gtgaattccc tgttgcccag gccctcgtat gggatgcatt caaagcggaa
     2641 agtggcaggc atgatttctt tgcttttagc ctccacggac tccatgactg agcggctacc
     2701 gctgaagttt tgctcttcat attcagcact gcgttttgca tcgatggtaa gacgacggac
     2761 agcagagata gcctgtttga tgtccagcac attaccgtcg ctgtcatagg ctgtgaggaa
     2821 gtcggcccag tcttccagcc attcagcgag agacttctgg agttgttttt tgccgtcaac
     2881 gtcacgcaat gcgcgatatg gtgatgtggc tttcagttcc agcagtgcct tgttgtcagc
     2941 gtgtccaggt tgctccagag tgccgatatt gaacacagtg actgctttca tcgtttccgc
     3001 gttaatgaag cagcgggagt gctcatcggc ataccctttg ctgtattcaa caaagtcatc
     3061 aatactggtt gtggtcatga ctccacggaa acgaaaacgt tctgtcatga atcgttccag
     3121 actttcgata tttacattgt ccggcaataa cgcaacaggg cattccgtaa gcggcaaatt
     3181 ttcaccagaa aaagcagtag tggtgagttt tacaatttct ttaatggcat tgctatctaa
     3241 ctgagacatg tatatttacc tttaatttat ggatttgggg ataatgcgcg agcgttttaa
     3301 ggcggggatt attcagcgta aaacagcgtc atgttgaccc tgtttatcga aaagctgtcc
     3361 ctggtctttc tggaacaaag taagctcacc acctttattt acaaacatcg gggtgttagt
     3421 tgtgtcttct tctgatttac taccacgcat tgtcgggcgt gtgaatttta gtttatggga
     3481 aactgatacg cgattttcat caagtgaaga taattcgaat tcaacacata cctttccttt
     3541 tttgtttgtg ctgtttacgc caaaggcaac ttcacttaat accgcgccaa gtttgttaac
     3601 aaataccccg ccgtcgaggt cgttaatgaa aacattaaca tcagtttgat gctggttcat
     3661 attaatatct ccgtgtagag gtgtgttaag gcttcagttg ttcactgcat ttaaagcctg
     3721 gttttgtttg tgaattaaaa actatcgctg ccgtactgcc agtaacctgg gtcgtccgtg
     3781 tgatttgggt ttacgttttt gctgcgattg tctttgtctt tgagagtgga aaccatattt
     3841 gctaccgttt ctggtttctt tccttcagct ttttctttca gtgtctgccc tgtttctgca
     3901 atgaatggaa tgcggatttc catctggtaa ccgtctgcgc cagtctttct gtttgtggtt
     3961 aatactttct ggaggacgag tccgattttc tttccgtgaa actcaggtgc aacatattta
     4021 ctgacggaaa ccatatgctg cgttaactgc ccaattcctg tgcatcccat catggcgtga
     4081 ataatgctcg ccccaaattt attttcagtg ccgtcattct tctgaacaca aacactgaga
     4141 tattggattt tccgtccgtc gtcggattct ccagaaaact caatgaatcg ggcacctttt
     4201 tctgattgct tgagttctgc ttcggtgatg ttaatgatat gcgcacccgt ttcggtaata
     4261 aaaccacctt gtccggcggt cagtgctgat tcttcgttat aagtaaaaat cacttcatcc
     4321 attagttatt aaccccccat tgttctccaa atacaaaacc aatttccgcc agtgcttcgt
     4381 ccattttgtc gatgaactcc ggcaccatct catcaaaact cgccatgtac ttttcatccc
     4441 gctcgaccac gacataatgc aggccttcac gcttcatgcg tgggtcgtag ttggcgaagt
     4501 accaggcgtc cttgtctgta acccacatgc tgaattgcac ctgggccatg taagcaggct
     4561 tgatagcgtc aaagccgcca agccggaatt tcatgaagtc gcgggaagta aaagggcatt
     4621 taagctcaag accgttaccg tcgctgcata aaccgtcggg agagcaggcg gtgcgcatgc
     4681 tttcgtcacg aaaaattatt ggcgtttccg agacggtaac atccgcgata aattcgaaga
     4741 gggcacgggc atcatcttcg tattgctttc cccatgcgag cgacttagcg ttaacttccg
     4801 gtgccacgcc ggtacaaact tcagccagca gggtgtggaa gtatgagatt tttgtatcag
     4861 gccatttgct gccggaacgt ggctttgcta tcacattgtg aacttctgaa gcagtgataa
     4921 caccgagtct cagcttgtgc catccatcat cgccctggtc gaggctggta atatccacgc
     4981 cagttcgctg gagaataatt tctggtgtca tgacacggcc tcgctgttat tttccgtggt
     5041 ggcgtgaatt ttcgcttccg ccgtggcctt gtttctggca gctttctttt tgacaaaatc
     5101 aagcgttttg acagcttctt cctgactgag atattcatgt gatgcaatcg tgcgacggaa
     5161 tgttttggaa cataagggaa gcaggtcatc ccatgtcttg ttaatttcgg tgattgcctg
     5221 agtgatctca ttgataatct catctgatgc gggagtgacg tcacgctcag ggatgtgatc
     5281 agcattcagg atgatacctt caccggcctg agtgttcagg tagtcgatag ctgtatccag
     5341 gcgatcgcga cggggccagt atttgctggc gcgcttaaca atggcttttc tggccatttc
     5401 gtcggggaag ctatcccacg ggctacttcc tccgttattt ccggctttgc tgcacgccct
     5461 gatgacctcg atttccctgt tgctcatctc ttcggtcaga tagtcgcctt cggatgtttt
     5521 tacgacacaa taaccgccaa tgcgtgcgcc cctgtctacg aatgggttgt atttgtgcgt
     5581 gggggcgcag tcaataccgt tggactcgta aatgtccttc tcgtaaacaa gtttgcattg
     5641 cccccactga atggctcctg tgacctgtgc cagatgcaga agccccatat aactgatatc
     5701 caggcataca gccttttttc gtgggaccag atacgccagc ttgctggccg ggttcagggt
     5761 tatgccgatg gcggcaacat tgatgatagc gttctgtgcg ctgggcagat ttgcccttgc
     5821 tgtgtcagcc agaaaggcat ttttctggaa ttgctgaatt gcaaactgac tttccttcgc
     5881 ccatgtcagc gtcggttcag ttaatgcctc gcagaaaaag cgctcctgct gcttaacaaa
     5941 ttcaacgata tcgaacattt tttggtcctg aaaatcagaa aggacagggg gagaattttc
     6001 tctcccattc ttcttccgcc cgagcatagg cgatcgctga gatataatcg ttgtacgcct
     6061 cttcagcttt ttcgccagtg agtgccagtt gggcttcttt gggtaaaaaa aggctgctca
     6121 taagcaatgg tttatcgggg aacatgctga taagctcctg cgcccgatca tcaatccatt
     6181 tatccttttc atcctgaatt tgctgattaa tccagcgacg ctcctctatg cggtcgcagg
     6241 tgaggtatgc gttcatggcg gaactcctga ttccggttaa tgcattaaat taatttgtcg
     6301 ggaaagctga catacagggc agttacattc ttcctcctgc tccttagcga agaaatatgc
     6361 agcggcctgt aatgcgatgt cttctggatg ttccgcgata aacataacat tgccttccgt
     6421 atcaataaca gaaatagcct catcagacag gatgacaaaa taggcgatga ttttatcatc
     6481 cataaaaact tctcccatta tcgttcctgc tggagttacg acgcttttta cattgatatt
     6541 tattttttga ttgagcatga tatttccttt caggctggtg agattaacgg ttggccttta
     6601 ttgttcaggt aaacttctat tgcatctgag ataatgcgaa ttttttcaat cagtgaatgt
     6661 gcgtaaagtg cattattaac gttcgctgac gccatgtaat aacgcccgtt gtaaagaatt
     6721 gctgtaccgg gtttaacgtc ctcgcgagaa actaatgcgg ttccgtagtg aggtttgagc
     6781 atgacaaatc ctccggttaa ttaatccaga tatttaattt aatccccgat atgtggtcgg
     6841 ggatggggtt aattaaagat ttacgttgaa accaaagcgg gaagactttt ctgatgtacg
     6901 ggaaatatcc agcaattgac ggcgcatttc ttccgtcaga tgcttgaagg cgttaaattc
     6961 ggcgacagcg gcatcgacgt tatagccctt gctgtgtagt tcgttgagga tgcgcatagt
     7021 tgggcttggc atatcgaaga gcacggaagc atcaaggctg ataaccttgc gatcaacata
     7081 gttcatcgtg gcgtagggat ggttatctga gaaccacgag agagggtagt tgatagatag
     7141 cgttgattct ggaagctgaa gttgttctg
//
LOCUS       DBFIFI010000217         6951 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.217, whole genome shotgun sequence.
ACCESSION   DBFIFI010000217 DBFIFI010000000
VERSION     DBFIFI010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6951
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.217"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..611)
                     /gene="yeeO"
                     /locus_tag="N7B39_004125"
     CDS             complement(<1..611)
                     /gene="yeeO"
                     /locus_tag="N7B39_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HGU2424005.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     FAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNIL"
     gene            772..847
                     /locus_tag="N7B39_004126"
     tRNA            772..847
                     /locus_tag="N7B39_004126"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:805..807,aa:Asn,seq:gtt)
     gene            complement(885..1835)
                     /gene="cbl"
                     /locus_tag="N7B39_004127"
     CDS             complement(885..1835)
                     /gene="cbl"
                     /locus_tag="N7B39_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HGU2424006.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLHNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPHDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIILSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEKNLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(1937..2854)
                     /gene="nac"
                     /locus_tag="N7B39_004128"
     CDS             complement(1937..2854)
                     /gene="nac"
                     /locus_tag="N7B39_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HGU2424007.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            3180..3255
                     /locus_tag="N7B39_004129"
     tRNA            3180..3255
                     /locus_tag="N7B39_004129"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3213..3215,aa:Asn,seq:gtt)
     gene            complement(3311..4246)
                     /gene="ldtA"
                     /locus_tag="N7B39_004130"
     CDS             complement(3311..4246)
                     /gene="ldtA"
                     /locus_tag="N7B39_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HGU2424008.1"
                     /translation="MMRRVNILCSFALLFASHTSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPVTVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(4308..5387)
                     /gene="cobT"
                     /locus_tag="N7B39_004131"
     CDS             complement(4308..5387)
                     /gene="cobT"
                     /locus_tag="N7B39_004131"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HGU2424009.1"
                     /translation="MQTLANLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALGV
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVNVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPFLNMDMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASKIVLPGNTTSDLNS"
     gene            complement(5399..6142)
                     /gene="cobS"
                     /locus_tag="N7B39_004132"
     CDS             complement(5399..6142)
                     /gene="cobS"
                     /locus_tag="N7B39_004132"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HGU2424010.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLVLMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(6139..6684)
                     /gene="cobU"
                     /locus_tag="N7B39_004133"
     CDS             complement(6139..6684)
                     /gene="cobU"
                     /locus_tag="N7B39_004133"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HGU2424011.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPEHWRTVERWQHLDELIHADINPNEVVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(6836..>6951)
                     /locus_tag="N7B39_004134"
     CDS             complement(6836..>6951)
                     /locus_tag="N7B39_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HGU2424012.1"
                     /translation="VPAVALIHYLNEMCQSTETAVNDAFCRTLTRESLAIP"
BASE COUNT         1876 a         1878 c         1528 g         1669 t
ORIGIN      
        1 ttaagaatat tcaggctacc gttaatcagt agcggtattt tcgtattccc tgcaccacga
       61 agtgccccgc taccaattag agtgatggca gctgctggat aactgagtac cgtcagctcc
      121 agataagtca acgccagtgc tttaacttct gtcgtggcat cacccgcgac gaaatcaata
      181 atttgctcgc caaaatgatg aataagcgtt gccaacagta cggcaaacaa cgtcatgatc
      241 accaatgact gccgcgtcgc caccctcgct cgtcgtcgat cccgcttacc gagactaaat
      301 gccacaacga cagtagtacc aagatcgata gcagcaaaaa aagccataat gaccatattg
      361 aagctgtccg ccaatcccac gccggccatc gcatcttttc ccagccagct gaccagaaaa
      421 gtgctcagaa cccccatcaa caggacacag gcattctcca tgaagatagg aacagcaaac
      481 ggggttatct cgcgccagaa caacactttg tagctcttgc gtttagcgtg ccagcgagtg
      541 ccgtgaacaa cctggcgtaa agcagaggag atattcaaag ccgaccttaa ttgcagaaag
      601 tgaaaccaca tttcaaataa tgagggagaa tcagcaaagc tgcaaagatt ttcgccatca
      661 aattgtctgc aaatgcaaca aactgttgat agaaacggca aacagttggg aaaattaaaa
      721 atcgggtttg acaaaagatt tttcgccgtt aagatgtgcc tcaacagcga ttcctctgta
      781 gttcagtcgg tagaacggcg gactgttaat ccgtatgtca ctggttcgag tccagtcaga
      841 ggagccaaat ttctgaaaag cccgctttta tagcgggctt tttgctatat ctgataatca
      901 atttcctctt cactgttttc catcacctgc cgcttgatat cctcaactga aagtcctgca
      961 ttgcaaagtt ccagaaagcg ccagacatag ttacgctgaa gttgtcctcg cttcagtccc
     1021 aaccagacag tattagcatc gaaaagatgc cgcgtatcca ggcggattaa attcttttcc
     1081 tcttgttcac cgctggattg ttcggcaact aatccgatcc caagcccaag agcaacatag
     1141 gttttaatga catcagaatc ctgcgcactt aatataatat ctgccagcaa acctttgcgg
     1201 gcaaatgcgt catcaatacg tgagcgcccc gtaatcccct gtcggtaagt gattaacggc
     1261 cacttcgcta ttgattccag cgtcaatggt gtaatttgcg tcaagggatg atcgtgtgga
     1321 acaagcaaac tatggtgcca acgaaaccac gggaaggcga cgagctgcgg gtcattactc
     1381 aaacgctcgc tggcgatacc aatatcagct tcaccattat gcaacaacgt cgcaatttcc
     1441 tgtggcgtcc cctggattag ctcgagccga acctccggga aaagttcgcg aaaagcttta
     1501 atgacctctg gcaagctata acgtgcctga gtatgcgtcg ttgcaatagt gagaacgcca
     1561 gacgtatcgt tggtaaacag gtctgcaagc cgacgaacat tactggcttc attgagaata
     1621 cgttctgcaa tgaccagtaa tgctttgccc ggttcagtca tgcccagcag tcgcttacct
     1681 cgtcgaacaa atatttcgat gccaagttca tcctccagtt cccgaatatg acggctgacg
     1741 cctgactgtg aggtaaaaag catattcgca acctctgtca ggttgtaatc ctgacgtgca
     1801 gcttcgcgga ttatctttag ttgttggaaa ttcacggtaa actccgggca gttcagatat
     1861 cccgttattg ttaaagtcta acgcccggca taacaaataa taaaaacccg catcttattc
     1921 catcccgata taacgcttag ctcaccaatt gccactgcct tttttccatc actggagaac
     1981 taatcactga cattaacaac tctttcactg cctgtgcctg tggcgataag ttcgctctgg
     2041 cgggtaaatt taatgacaaa gagagactca tggaaggcgt ggtaatgcgt gacatccacc
     2101 catttactgc gccacataac gaacgcgcgg ccgattcggg taatactgca acgcccatgc
     2161 cgctggcaat cgctgcggta agcgtagcaa tagactcaat ttcaccaatg acttttgccg
     2221 tgagccgccg tagggaaaac gcctcatcaa cacgaagtct aacagcgctg taatcactgg
     2281 ggagaaaaag gttcatttgt gcaatagcat taacatcaac gctttgcccc gggcaatctt
     2341 gagttcctac caggaaaaga tcttctttca gcaaagcctg actggataca ccagccacag
     2401 gggaatgctc ataaatcacc gccatatcga gttggtgatt tatcagtttt tcgttaagca
     2461 ctgcaccgct attttcatga agatagataa cgacctccgg gaattcagcg cgaaccgcct
     2521 gtaataaggg catggtgatg gatgacgcag cggttcctgg cgcaaagcca atcgagactt
     2581 gccccgataa tgcctgacca acgttatgca ccgccagttg ggcctgctca cactgacgta
     2641 aaatggcccg cgcatgggta tagagaattt ttccggcgtc tgttggcgta acgccccgtt
     2701 ttgtgcgaat caaaagttgt tgatttaact caccttcgag tgtggcaacc tgctggctga
     2761 gcgctggttg tgcgatatgc aatacttcag cagcctgagt caggctacca atatctacaa
     2821 tttttacgaa gtatttcagg cgtctgaagt tcatgttgcc tccggttttt gagaatcggc
     2881 ccaagtgccg ccattactta caaccagatt gcaagatgct tgccagtttt attttggcgt
     2941 tgatgtacaa gctaaccaac tgtcaaataa gagattatga tagattcgtc attggctctt
     3001 ttcaccagca gtcgcgttcc cctgccctat aaaaggaggg tatgcaccac gatggttcat
     3061 taccaataag attgaaatct catcactttg ttgaaattga cagcaaacaa acaaaaaaat
     3121 gcatttcacc ctttgacatc accatgcact gccattaata tgcgccccgt tcacacgatt
     3181 cctctgtagt tcagtcggta gaacggcgga ctgttaatcc gtatgtcact ggttcgagtc
     3241 cagtcagagg agccaaattc aaaaaagcct gctttctagc aggctttttg ctttctaatt
     3301 accaacgctc ttaaaacatc tgtcttgaac cagaactaat ttgcacaggc attcccgatc
     3361 gacgttgcaa cgcagcattt gcgcgattca catcaacttc ttgcccgttg ataaacgccc
     3421 gcaaagatgg ggttaccggc aatggcactt ttcggtcaga ctcatattca gcacgattgc
     3481 gcgacagcgg ctcatgaact tccagccact ttgaaccatc cggttccgtt gtgtatttca
     3541 ctggctgatc aataatttgc acacgagtac ctacaggaac attatcaaac agatatttga
     3601 tatcgtcatt gcgcagacga atacagccct gacttacccg gagcccaata ccaaaattgg
     3661 cattggtacc gtggatggca tatagcctgc caatataaat cgcgtacagc cccatgggat
     3721 tatcgggccc cgcaggaaca aatgcgggca aactctcccc tcgtttcgca tattcgcgcc
     3781 gggtgttcgg cgttggcgtc caggttggtg cttcttgttt acgttcaacg gtagtcaccc
     3841 agttacgcgg ggtttctcgc ccagcctggc cgataccaat aggaaagact tccacagtat
     3901 tactgtctgg tgggtagtaa taaagacgca tctcagcgac gttaaccaca atccctttac
     3961 gaacagtgac gggcaaaatc agttgctgcg gaatggtgag ttgcgaacct gacttcggta
     4021 agaagacatc agcgcccgga ttcgcttcca gcatgttact taacccttgc ccgtattgtg
     4081 cggcaaaagt ctccagcggc tgggtattgt gatcaggaac agttacagta agcgactgcc
     4141 ccactaaacg gctaccctct ggaggtaatg gataagttac cgccaggcta gtatggctgg
     4201 caaaaagcag agcaaatgag caaagaatat ttacacgacg catcatgtcc ctttcctaag
     4261 tcgcgaaagc tatcggttaa gtatagcttt tatcagactt ttcgttttta actgttcaaa
     4321 tcagaagtcg tattccccgg tagaacaatt ttactggcag caagttcgcc catgttgttg
     4381 tatatcgcac aagcagcttc gatgatgggc atcgccagag ctgcgccact cccctcacct
     4441 aaacgcatat ccatattgag aaaaggctcc agccccaaat gcgacagcgc tatacgcgcc
     4501 cctttttctg ccgacaggtg agaaggaatg agatacggtt tgattgcagg agacatctgg
     4561 caggctgcga gcgcagcagc ataagaaaga aatccatcca gcaacacggg taaaccgcag
     4621 gaagcagcgc ctaacatcac gccagccatt ccgaccaaat caaatccacc cacttttgcc
     4681 agaacattaa cgccatcctg cggatttggc tgattcaacg taatcgcccg gcgcacaaca
     4741 tcaattttat tagccagttt atctgtcggc aggtttgcgc caatcccaac cacttcttca
     4801 ggatcccggc cagtgattgt gctgactatt gctgctgccg gtgtcgtgtt tgccatcccc
     4861 agttcaccta caccaaacag cgtgacaccg ttttttgcca gctcccgcgt ataacatatg
     4921 acgtccaaaa gtaacttttc agcctgacgg cgactcattg ccggagctga agcaatattg
     4981 ccgctacctc gtgcgacacg catgttgata agcccgggga taggctcagc agtatcaata
     5041 ccaacatcaa ttacgtggac gttagcgccc gcttgtgctg ccagcacaca cacgccggtt
     5101 gttccgcggg tcatattttc agcctgaatg gctgtcactt cttttgggga aatagcgacc
     5161 ccttcctccc agacgccgtg atcggcacac ataaccagta ccgctttttt gcccacatgc
     5221 ggtatgccat tcaaccccgg cattcccgcc agttgtacgc caagcgcctc cagccttccc
     5281 aggctaccaa caggtttgag taacccgtca atatgccgtt gtgcacgcga catagcggca
     5341 ggatcgatgg cagggatcgt attcagtaaa ttggcaagtg tttgcatgtc acgtctcttt
     5401 atagcagtgc cagcaggaag accagttcac caagctctat ggctgctccc agcgtatcgc
     5461 cggtttgccc gcccagcgta cgtttgagaa gctgaccgag gatgaaaatc gccaccatcg
     5521 tcaccaccat agcagccaca ccatgcatac ccggcaataa tacggcagca aaaatagcgg
     5581 ctaagccgag tgtgacgcag gtttgtcgcc catcaatttt gccaatgaac acattgccaa
     5641 gcccttcttc ccgggcataa cgatgacgat acatcaataa tgcggcagtc ccgcggctga
     5701 ccgcacatgc tgccgccagc gatgcaagga tcggttcgcc acgcagagcc aactcgctta
     5761 acaccaaaat ctttgccagt acaacaaaaa tcaatgccag accgccgtgg gtacctaaac
     5821 ggctatcacg catgatttcc aacatgcgat cgcggctacg tgcagaaaat acgccatcac
     5881 aggtatcagc gaggccgtcc agatggaatc ccccggtcat cagcaccagc acaagtacgc
     5941 taaacagtgc cgccagtggt gcgccacacc atgcctgcag caccatgaag accagcccgc
     6001 taatcgcgcc aagcaataat ccaatcaaag gaaaagtaat aataccgcga gaataatgct
     6061 cgaaatccag tccctgggac cagcgacgcg gtacaggcag acgcgtaata aatgagagca
     6121 tcgcccaaaa taatttactc atttgatttt tactccaata cccgaaacca ccagccatac
     6181 ttcatttgcc gcagcggcca actgctgatt tacccgcccg gcaatatcac gaaaatgtcg
     6241 tgccagacga ctctccggca caatccccat tcccacttcg ttagtcacta atacaacctt
     6301 tgcggggcaa cgttggcagg cagcaatcaa cgactgaatc tcagcattaa tcgcctgttc
     6361 catcgcctga taatcccatt catcagggtc tttatcgccg ccataatcaa acaacagatt
     6421 agtcaccatt gtggtaacgc attcaagcaa cacaacctca ttcgggttaa tgtctgcatg
     6481 aattaattca tcaagatgtt gccagcgctc cactgtgcgc cagtgctccg ggcggccttg
     6541 ccgatgatgt tctatccgtg cagccatctc atcatcaagg atttgcgagg tagcgatata
     6601 cagaacctgt gaagagtccc caataagcgc ctctgcgtgg cgactcttcc cgctccgtgc
     6661 gccccccgtc accaaaatca tcataccgac tcctgattca aatgtatgat ggtattcatt
     6721 gaacagaaca atggatcccc caccgttgct gaccgcaaaa catttacgcg attaatactg
     6781 cgcgtaatat aattaaaaat ccagcattct caatccatta cgaaataaga tggcattacg
     6841 gaatagccag actttctcgt gttaacgtcc gacaaaatgc gtcattgaca gcggtttctg
     6901 tggattgaca catttcattc agataatgaa ttaatgctac tgccggaaca a
//
LOCUS       DBFIFI010000218         6877 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.218, whole genome shotgun sequence.
ACCESSION   DBFIFI010000218 DBFIFI010000000
VERSION     DBFIFI010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6877
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.218"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            5..565
                     /locus_tag="N7B39_004135"
     CDS             5..565
                     /locus_tag="N7B39_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2424013.1"
                     /translation="MFSTFKKAALLAAIALPFSTMAAPTVTFQGEVTDQTCSVNINGQ
                     TNSVVLMPTVAMADFGATLADGQSAGQTPFTVSVSNCQAPTGADQAINTTFLGYDVDA
                     STGVMGNRDTSSDAAKGFGIQLMDSSTSGNPVTLAGATNVPGLTLKVGDTEASYDFGA
                     RYFVIDSAAATAGKITAVAEYTLSYL"
     gene            651..1352
                     /locus_tag="N7B39_004136"
     CDS             651..1352
                     /locus_tag="N7B39_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309306.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HGU2424014.1"
                     /translation="MNIFAYLLVLVFSMSMSSSAFASVVMTGTRIIFPGDAKEKTIQL
                     RNTSDQPYIINIHVEDERGSDKNVPFMPTPQTFRMEAAAGQALRLLYTGNNLPQDRES
                     VFWFSFSQLPYLNKNDKSQNQLILALTNRVKIFYRPSSIVGKSSDAPKNLTYQVKQNR
                     IEVTNPTGYYVTIRAAELLNNGKKVPLANSVMIAPQSTTEWTLPSGISVAPGAQIHLV
                     TVNDYGVNVTSEHAL"
     gene            1405..3922
                     /locus_tag="N7B39_004137"
                     /pseudo
     CDS             1405..3922
                     /locus_tag="N7B39_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309305.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            3938..5272
                     /locus_tag="N7B39_004138"
     CDS             3938..5272
                     /locus_tag="N7B39_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2424015.1"
                     /translation="MSKSTFLHILISSIILVALIQSSAWANCTNTQIGQTEDGRTALI
                     EFGKINMTDTYFAPAGSLLATTVVPPTNYTSGGATGSSVLWECDATDLPNIYFLVATN
                     GDDRVGGFYDAGGPDGLSDVYATWFAFVGLKQTMAGVTLGRYWKKVPITSYATQGTKI
                     QIRLQDIPPLHAELYRISTLPDTSATTSWCGNNNTDSSGVGFAKPSGTIYNCVQPNAY
                     IQLSGTSGILFGHDEPGEDSSVHWDFWGADNGFGYGMRSANRLYNNATCVARSATPLV
                     LLPTIAEAQLNAGMESTGNFNVRVECSNSVQSGISDTQTALGIQVSEGAYTAAQKLGI
                     INSNGGVSALVSDNYDAAEMAKGVGIYISNSAHPDTAMTLVGQPGIAKLTPGGNAAGW
                     YPVFEGATLEGATHPGYSSYSYSFIARLKKLPNQTVSAGKVRATAYILVKMQ"
     gene            5269..5958
                     /locus_tag="N7B39_004139"
     CDS             5269..5958
                     /locus_tag="N7B39_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HGU2424016.1"
                     /translation="MKSVLLLLTILFVQSVEAGIMPASSRIIYRDGDREKTLMLVNTN
                     DYPVLVQSWVDNGEGSPEAASPPFVVLPPVFRLAARRTQGLRIIYNHDPLPQDRESAF
                     WLNLYEMPPETPHQDPTHLTLAMNTQLKIFYRPKSVTMTPQEAMRKISWHLGNDGSSS
                     YIELNNPTPLNISFTSISIGATKVQQESDMMIKPFSQRRYWLASNNVSGVVAATYLDD
                     NGAQIKQQLAQ"
     gene            complement(6104..>6877)
                     /locus_tag="N7B39_004140"
     CDS             complement(6104..>6877)
                     /locus_tag="N7B39_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU2424017.1"
                     /translation="SIEDFSLDFFSLHNKVAIVTGAAGELGRGLCSALAKAGANLLLV
                     DIKEPDNRYLKHLTHEGVEVEFMTIDITKPDASCTIINRCLERFGQLDILVNNAGVCN
                     INRPIDFNRNDWDPMINLNLNAAFDMSQAALNIFVPQRKGKIINMCSVLSFHGGRWSP
                     GYAATKHALAGLTKAYADDFAEYNIQINGIAPGYYVSEMTAIIYNNPKIKELIKGRIP
                     AQRWGRAQDLMGAMVFLASAASDYVNGQLLVIDGGYSIR"
BASE COUNT         1834 a         1705 c         1628 g         1710 t
ORIGIN      
        1 taatatgttt tctactttca aaaaagcagc tctgctggca gctattgcat tacctttttc
       61 aactatggct gcgcctacag tcacttttca gggtgaagta accgatcaga cctgttccgt
      121 aaatatcaac ggtcaaacca attcagtagt attgatgccg accgtagcca tggctgactt
      181 cggtgcaact ttagctgatg gtcagagcgc aggccagacg ccgtttacgg tttctgtgtc
      241 taactgccag gctccaactg gtgcagatca ggcaatcaac accaccttcc tgggctacga
      301 cgttgacgct agcacgggtg ttatgggaaa ccgtgatacc agcagcgatg cggcgaaagg
      361 ctttggcatt cagttaatgg attccagcac ttctggtaac ccagtaactc tggctggcgc
      421 gactaacgta ccgggtctga ccctgaaagt tggcgatacc gaagccagct acgacttcgg
      481 tgcgcgttac ttcgttatcg atagcgctgc tgccactgcc ggtaaaatta ccgctgtcgc
      541 agaatacacc ctgagctacc tctaattaat ctactggctg gcagtaaatg ccagccagta
      601 tttcttcgca caattaatag tgatttttca tggacatgga aaagataaga atgaacattt
      661 ttgcatattt actggtactt gtattttcca tgagcatgag cagcagcgcg tttgccagcg
      721 tggtaatgac cggaacccgt attattttcc ctggtgacgc aaaggaaaaa accatccagt
      781 tgcgaaatac cagcgatcag ccctatatca ttaatatcca tgttgaggat gaacgtggtt
      841 ctgacaagaa tgtaccgttt atgccaaccc cgcagacatt tcgcatggaa gctgccgcag
      901 gtcaggcgtt acgcctgctc tacactggta ataatttacc gcaggatcgc gagtctgttt
      961 tctggtttag tttcagtcaa ctaccttatc tgaataagaa tgataaaagt cagaaccagc
     1021 tcatcctggc cctgactaat cgagtcaaaa ttttctatcg tcccagctcg attgtcggta
     1081 aatccagtga cgcacccaaa aacctgactt accaggtaaa acagaaccgc attgaagtga
     1141 cgaatcccac gggctattac gtcacaattc gcgccgctga actgcttaat aatggtaaaa
     1201 aagtccccct cgcgaattcg gtaatgattg ctcctcaaag cacaactgaa tggacactac
     1261 cctctggcat cagtgtcgct cccggtgcgc agatccattt agtgaccgtc aacgactatg
     1321 gcgtaaatgt tacgtctgag catgccttat aacctctttc tccttttttg agcggtatcc
     1381 tttttcacgc acgagtacgt cgagatgaat aaatccgttg tgtcaatttc tgcggcaatg
     1441 ttggttttac tttgccaacc ggtcatgggg agcgaaatct cacccgcaac accgtcagat
     1501 gaagacaact acacctttga cccgcaactc ttccgcggca gcagatttag tcagtcgtca
     1561 ttagcaaaac tgacaacacg tgagtctgtt gcaccgggca attataaaat ggatatctac
     1621 accaacaata agttgtcagg cagttggaat gtcacgttta aagaagccgc tgatggtcgc
     1681 gttctgccct gcctgacgcc tgaagtcgcg gacgcgatag gcctcaaaac aggggaagat
     1741 aagggggaaa aagatcctgt ctgtacgttt gctaaggaac tcgctcccgg catcaccagc
     1801 cagacacagt tgtcacaatt gcgcctggac ttatcggtgc cacagagtca attgattagt
     1861 cgccctcgcg gctatgttcc ccccagcgag ctggataccg gagcatcgct ggcgttcatg
     1921 aattatattg ccaactatta caacgttgcc tattcagggc agaatgctca tagccagcgt
     1981 tcgctatggg catcatttaa tggtggcatc aaccttggtg cctggcaata tcgtcagtta
     2041 tccaacatga cctgggataa tgacaaaggg aatcagtgga acaatattcg tagctatttg
     2101 caacgcccgc tgcccgccat aaatagccag ttaatgatgg ggcagcttat caccagcgga
     2161 agatttttct ctggactcag ttatcacggc gttagtctcg cgaccgatga acgtatgctg
     2221 ccggactcca tgcgcggcta tgcgccgact attcgcggcg tggccgcaac aaacgccaga
     2281 gtctcggtaa tgcaaaacgg tcatgaaata tatcagacca ccgtggctcc tggccctttc
     2341 gagataaacg acctataccc caccagctac agcggcgatc tggatgtcac cgttacggaa
     2401 gctaacggcg cagtcagtcg tttcagtgtc cccttttcag ccgtaccaga atcgatgcgt
     2461 ccaggaactt cccgttataa cgtggaagta ggtaaaacgc aggatagtgg tgatgactcg
     2521 atgtttggtg accttacctg gcagcacggg atgactaata cgctgacatt taacagtggt
     2581 tcgcgtatcg ctgatggcta ccaggcgctg atgctgggcg gagtctatgg cagttcgctg
     2641 ggggcatttg gggcaaacct cacttggtcc catgcgcgtg ttcccgaaag cgaagcgcag
     2701 agtggttgga tgtcgcaatt aacctggagt aaaactttcc agcctacttc aaccaccgtc
     2761 tccctggcag gttatcgata ctctaccagc ggctatcgtg atctggctga tgtgctggga
     2821 gagcgtcatg ctgccagcaa taaacagtca tgggactcca gccagtggcg tcaacagtcg
     2881 cgcttcgatc ttacgttaag tcagagcctt gcgaattacg gcaacctgtt tgtgtcaggt
     2941 tcaacacaga actaccgtgg cggcaagagc cgtgatacac agcttcagtt aggttacagc
     3001 aatagcttta gccatggcat cagtatgaac cgtcggacgc caaagaatgg gcggctataa
     3061 agacaattct gatgatatgc agacggtaac atccctttca ttctcattcc cacttggcgg
     3121 caatggacct cgtgtaccaa gtcttagcaa cagctggacc cattcaactg acggtagctc
     3181 gcaattacaa agctcgctaa ccggaatgct tgatgaagca cagaccacca actacagcct
     3241 gaacgtcatg cgcgatcaac aatataagca gacgacgctt agcggaaaca tgcaaaaacg
     3301 tttttcacaa actaccgtcg gattgaacgc atcgaagggc caggattact ggcaggcttc
     3361 aggtaacgta caaggcgcga tggctgtgca tggtggcggc attactttcg gaccttatct
     3421 gggtgaaacg ttcgccctgg tcgaagctaa aggcgcagaa ggtgcaaaag tctataactc
     3481 cagtcagctg gaaattaatg acagtggcta tgcgcttgtt ccggcagtaa cgccctatcg
     3541 ctacaaccgt atatctctcg atccacaagg aatggatggc gatgccgagt tggtcgacag
     3601 tgaaagacag gtagcaccgg ttgcgggtgc ggcggtgaaa gtaattttcc gtacccgtcc
     3661 tggtaaagcg ttgctgatta aatcccgcat ggcagatggt tcggaactgc caatgggagc
     3721 cgatgtgctg gatgagaata atacagtcgt cggtatagcc ggtcaggggg ggcaaattta
     3781 cctccgcaca gaacagacaa aaggccactt gtcagttcgc tggggtgaag gtgctaacga
     3841 tagctgccaa ttgccctttg atatcagcgg gaaggacagc aatagcccta tcatccgcct
     3901 gaatgaaacc tgtcagtctt gatacagaga aaacccgatg tctaaatcga cttttttaca
     3961 cattctgatt agctccatca tattggtggc actaatacaa tcatccgctt gggctaactg
     4021 tacaaacaca cagataggtc aaactgaaga tggtcggaca gcgcttattg agttcggaaa
     4081 aattaatatg accgacacct attttgcgcc agcaggttca ctcctcgcga caacggtcgt
     4141 acctcccact aactacacat caggtggcgc gacaggaagc tccgtattgt gggaatgtga
     4201 tgcaacagat ttgccaaaca tctattttct ggtcgctacc aatggcgacg accgcgtggg
     4261 gggcttttac gatgcaggcg ggcctgatgg tctgagtgat gtctatgcca cctggttcgc
     4321 ttttgtcggt ctcaagcaga ccatggcggg cgtgacgctt ggtcgttact ggaagaaagt
     4381 gcccatcacc agttatgcca ctcagggaac taaaatccag attcgcttac aggatatccc
     4441 tcctcttcat gctgagcttt atcgcatcag tacgctacct gatacatcag caacaacaag
     4501 ttggtgcggt aataataata cagatagtag tggagtcgga ttcgcaaaac cttccggtac
     4561 aatctataac tgtgttcagc ccaatgccta tattcagctt tccggtacca gcggcatttt
     4621 atttggtcat gatgagcccg gcgaagatag ttctgttcat tgggatttct ggggtgctga
     4681 taatggtttt ggttacggaa tgcgttcggc caatcgactc tacaacaatg ccacctgcgt
     4741 tgcccgcagc gccacgccgt tagtattgct gccgacaatt gcagaagcac aactgaatgc
     4801 gggcatggaa agtaccggta attttaatgt ccgcgtcgag tgtagtaact cggttcaatc
     4861 agggattagc gatactcaga cagcattagg aatccaggtg tctgaaggtg catatacagc
     4921 ggcgcaaaaa ctggggatta tcaatagcaa cggcggcgtc agcgccctgg tctctgataa
     4981 ttatgacgca gcagagatgg caaagggcgt tgggatctac atttctaaca gtgctcaccc
     5041 cgatacggcg atgacgctgg ttggtcaacc gggcatcgcg aagttaaccc ccggaggaaa
     5101 tgcagcgggg tggtatcctg tatttgaagg ggcaacatta gaaggtgcga ctcaccccgg
     5161 atactccagc tatagttact cttttatcgc ccggttgaag aaactgccaa atcagacagt
     5221 cagtgcggga aaagtgcggg caacggctta tatattggtg aaaatgcaat gaaatccgtt
     5281 ttgctgctct taaccatctt gttcgtacag tcagtggaag ccggcatcat gccggcttct
     5341 tcacggatta tttatcgcga tggcgatcgg gaaaagacac tgatgctggt caataccaat
     5401 gactatccgg tactggtgca aagttgggta gataatggtg aaggttcccc agaagctgct
     5461 tctcccccct ttgtggtgct gccaccggtt ttccgcctgg ctgcgagacg aacccagggg
     5521 ttgcggatta tctataatca tgacccactt ccccaggatc gcgaatctgc attctggtta
     5581 aatctttatg aaatgccgcc agaaactccc catcaagatc ccacacattt aacgctggca
     5641 atgaatacgc aacttaaaat cttttacagg ccaaaatctg taactatgac gccccaagaa
     5701 gctatgcgta aaataagttg gcatctgggt aacgacggca gcagtagcta tattgagctt
     5761 aataacccaa caccactgaa tatctctttc acctccatca gcattggggc tacaaaggtg
     5821 cagcaagaaa gcgatatgat gataaaaccc ttcagtcaac gacggtactg gttagcaagt
     5881 aataacgtta gtggtgtggt tgcggcgaca tatctcgatg ataatggtgc acagataaaa
     5941 caacaattag cgcaataatg ctctgagtgg tatgtaaact catcgcgatg gttcaacgct
     6001 aacgaataac cactataccc acctgaagta ccggaagtgg agaaatgaca aaatctctcc
     6061 actccatttt ccagtcaaaa cacctcaacg cagccaaaaa tcgttaacga attgaatatc
     6121 caccatcaat gactaatagt tgtccattga cataatctga agcggcactg gcgaggaaca
     6181 ccattgcgcc cataagatcc tgtgcccttc cccagcgttg agccggtata cgccctttta
     6241 ttaactcttt tattttaggg ttattataaa taatagcggt catttcactc acataatatc
     6301 ctggggcgat accattaatc tgaatattat attccgcaaa atcatcggca tacgctttgg
     6361 tcagccccgc taaagcatgt ttagtcgccg cataccctgg cgaccaacgt cccccatgaa
     6421 atgacaatac ggaacacata ttaataattt tcccttttct ttgcgggaca aatatattca
     6481 atgcggcctg gctcatatcg aatgcagcat tcaggttgag attaatcatc ggatcccagt
     6541 cgtttcgatt gaaatcgata ggacgattaa tgttacacac gcccgcgtta ttgaccagaa
     6601 tatctaactg accgaaacgc tccagacacc tattaataat ggtgcatgat gcatcaggtt
     6661 ttgttatgtc tattgtcata aactcaactt caacgccttc atgagttaaa tgcttcaaat
     6721 atctattatc aggctctttg atgtcaacca acaatagatt agcccctgct ttggccagag
     6781 ccgagcataa gcctctcccc agctcacccg cggccccagt tacgatggcc accttgttat
     6841 gtaatgaaaa aaaatccaat gaaaaatctt caataga
//
LOCUS       DBFIFI010000219         6813 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.219, whole genome shotgun sequence.
ACCESSION   DBFIFI010000219 DBFIFI010000000
VERSION     DBFIFI010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6813
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.219"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(219..704)
                     /gene="sixA"
                     /locus_tag="N7B39_004141"
     CDS             complement(219..704)
                     /gene="sixA"
                     /locus_tag="N7B39_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HGU2424018.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGKGEFNWQMSPCNLKMAKA
                     I"
     gene            complement(907..3051)
                     /gene="fadJ"
                     /locus_tag="N7B39_004142"
     CDS             complement(907..3051)
                     /gene="fadJ"
                     /locus_tag="N7B39_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HGU2424019.1"
                     /translation="MEMTSAFTLNVRLDNIAIITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTEVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGIP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVAHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3051..4361)
                     /gene="fadI"
                     /locus_tag="N7B39_004143"
     CDS             complement(3051..4361)
                     /gene="fadI"
                     /locus_tag="N7B39_004143"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HGU2424020.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVVESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQLLAEDNNIRGNSSLADFAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     ADLTLIDMHEAFAAQTLANIQLLGSERFAREALGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4541..4825)
                     /locus_tag="N7B39_004144"
     CDS             complement(4541..4825)
                     /locus_tag="N7B39_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HGU2424021.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5197..6537
                     /gene="fadL"
                     /locus_tag="N7B39_004145"
     CDS             5197..6537
                     /gene="fadL"
                     /locus_tag="N7B39_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HGU2424022.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGKTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRVFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
BASE COUNT         1701 a         1835 c         1607 g         1670 t
ORIGIN      
        1 ttttttatta ttaactgttc tattagtttg atgctttgta aaaatacatt acaacttagt
       61 ctcatttcgt cagaaattaa tagggaagca gaggaaaaag tgcgatgaat tctgtatcga
      121 agcgtatcaa ctccgtgaag ggttttgagt gaatttctac tattgcaaaa agacaaatgc
      181 ccgatgtaca gcttacatcg ggcatttttt caaacgaatc agatagcttt cgccatcttc
      241 aaattacatg ggctcatctg ccagttgaac tcgccttttc cgctttcatc aagcgttacg
      301 ctggcaatgg ccgaggtggt aaacataggc ggcgtttcgc ccggacataa ctcggcgacc
      361 agatacccaa ctaacggtaa gtgggagata accaacaccg aagcaacacc ttcattggtc
      421 aacgcctgca aataggcact gaccagacca acatcgccgc agggcgttaa ctccggcaga
      481 acttctgcac tggaaggcag gttcagacaa tccccaactt cctccagtgt ttgctcggct
      541 cgcaggaacg ggctcaccag aacgcgttcg atttccactt tttgaccttt cagccagttc
      601 gccatcaggc gagattcgtc acaaccgtta gtggtcagag gacgaaccga atcactggcg
      661 gcatcgaggg ctgcgtcgcc gtgacgcatg ataaaaactt gcatattgca ccgcttttgt
      721 taaccagatt cgcccgtttt ctttcatctg aaaccagaga aagaaaacgg tggccggcat
      781 tgtgccttat ccattcaccg aatgaaacgc tgttttttac ctcaatggcg taagtatagt
      841 caatatgcgt ttacattttg accaacaatc cgccattcag cgcggattca tatagctttg
      901 accttcttat tgcaggtcag ttgcagttgt tttccaaaaa ctttccccac gcgcacccat
      961 ctcgactaaa cgctcgcaag gggtaaaacg tgaaccatac tgcgtggcca gtcgttgcat
     1021 tattgcaacc acttcgcccg cgccgagaga atcgatatag cgaaacggtc caccgagaaa
     1081 tggcggaaaa ccaatgccaa ataccgcacc aatatccccg tcacgcacgc tacggataac
     1141 ctgctcatca acacaacgta cagcttcatt cagcatcaac atcacacacc gttcagcaac
     1201 ctgcggtgcg gagagtcgcc cctgcccttg tgcgccaatg agcgggtaaa tggcaggatc
     1261 gacctgtttt ttgcttttac gccctttctg accataaaga tagaaacctc gaccattttt
     1321 tctgcctttg cgatcgtcgt tcaaaattga agaaacaaca tttgcaggcg cgctaaaacg
     1381 ttctccataa gcggcttcca gtacaggaat aattttagtc ccggtgtcga ttcctacctc
     1441 atccaaaagt tggattggac ctaccggaaa accaaatttc actagcgcgg catcaatgtg
     1501 cgctacccgt tcaccttcgg tcagcatgcg gatagcttca ttaatgtaag gcgctaagat
     1561 gcgattgacg taaaacccgg ctttgtcacg cacgacaatg ggcgttttgc cctgtttttt
     1621 cgccagtttt accgtagtgg cgatggtttg tgctgatgta cccgcatgag gaataatctc
     1681 caccagcggc attttttcca ccggactgaa gaaatgcagg ccgataactt gctcaggtcg
     1741 cgtggcgtga gcggcgatat caccaatcgg taaagatgac gtattcgaag caaagatggt
     1801 atgagcggcg caattttgct caacttccgc caccatctgt tgtttcaatt cgagattttc
     1861 aaacaccgct tcaataatca gatcgcgatg ggcaaagccg cgatagtccg tcgttccgga
     1921 gattaatgcc agctgtttgt cacgttcgct ggctttgaga tgacgacggc gaactttgcc
     1981 ctccagctga tcccaactgt acttcagcgc atgatttatg ccctgcgggt tgatatcttt
     2041 aattctgacc ggaatccccg ctttacaagc agtgacataa gcaataccgc cgcccatcaa
     2101 gccaccacct aaaatcccca cgctgtttaa tggcgcaggc ggcgcatcac tgccgggatc
     2161 tttcttcact tccgtactgg caaaaaagat actacgcaaa gcttgcgatt gtggcgtcat
     2221 cgccagttcg ccaaacgccc gtgcttcagc gtcatagcca ctgctggtgc cctgcgctaa
     2281 tcccgtttca acaacctcca ggatgcgttc tgtcgccgga taattgcctt gagttttgtg
     2341 ttctgttttc ttgccgacca ttttgaacag cagcgcacga cctaacggcc ccgccagaat
     2401 acgctcgcgt acaggtagag ggcgggaaga tgggcgatcc ttctttgcca gctcaacagc
     2461 ggcttccagc agaatggagt gcggaacaac atcatccacc agccccagct ttaatgcctg
     2521 tttcgcccga agttgttttc cggtgaggat catctctaat gctgtgctga cgcctatcag
     2581 acgcggtaaa cgctgggtgc cgcctgaacc gggtaacaat ccaagttgta cttcaggcaa
     2641 accgagcacc gttttaggat cgtcagtaca aacgcgaccg tggcacgcca gcgccaactc
     2701 cagcccacca cccaggcaag cgccatgaat agccgcgata accggaatgg gcaaagcatg
     2761 aatctccgcc atcaactgtt ggccctgccg cgccagagct tccgcttctt gcgccgtttt
     2821 gcaattgccg atcatgttga tgtctgcgcc agcaatgaag ttgtccggtt tagcggagac
     2881 aaacaccacg cctcgcaact ctttgttttc acggagttgc ttaataatgg cgcgcacctg
     2941 cgaggcaaac tccgccttca gggtattcat tttctcaccc ggtacgtcga tggtgatgat
     3001 agcaatgttg tccagacgaa cattaagagt aaacgctgat gtcatttcca ttattccgcc
     3061 tccagaacca ttgccgcgcc aagcccaccg gcagcacagg cggtaactaa accaaatcca
     3121 ccgccgcgac ggcgaagttc atgcaatgtc tgggtaatca tccgcgcgcc ggtcgccgcg
     3181 aagggatgcc cgtaagcaat cgaaccgcca agcacgttaa atttgctatc gtccacttcg
     3241 ccagtggcat gtgcacgccc cagcgcttca cgagcaaaac gttcactacc cagtagctga
     3301 atattggcca acgtctgggc ggcaaaggct tcgtgcatat caatcaatgt cagatctgcc
     3361 attgtcaaac cggcacgctc cagcgccagc ggtgttgacc aggctggacc gagcaacatg
     3421 tcctgccaga catcgatagc ggtaaatgcg tagctgcgca gatatcccag cggtaccagc
     3481 cctaattctt tcgcccggga ttcagtcatc aggatcaccg ccgctgcgcc atcggtcagc
     3541 ggcgtactgt tcgccgccgt taccgttccg tgtttacgat caaacgccgg acgcagcttt
     3601 gcgaaatcag caagcgagga attaccgcga atattgttgt cttccgcgag cagttgttta
     3661 taaggaggga taaaggcagt catcacctct tctttgagtt ttccgtctga ccatgcctga
     3721 gcggcacgct gatgcgaacg gtgcgctaat gcatcttgct gttctcgggt gatgccgtag
     3781 gttttcgcca tttgctctgc agtgtcgccc atccgcaagc cggtagaata ttctgctacc
     3841 gcaggtggta cgggcattaa gtcgcgcaaa cgcaggcgag agaagagttt cagacgctgg
     3901 ctcattgtgc gggctttgtt gacatcaacc agcacgcgcg ccagtttttt actgacgcca
     3961 attggcaata ccgaagagga atctgcccca ccggcaatcc ccgctcggat agtccccgcc
     4021 atcaggcttt ctacgacgtt tgcaaccgcc tggaaactgg tagcgcaagc gcggctgacg
     4081 ctgtaagcat cggtatgcac attcattcca gtaccgagaa caatttcacg cgcaatgttg
     4141 ggagcttcag gcatttgtac gacctgacca aagaccagtt gttcaatcac ttcggcgggg
     4201 atctcgctgc gtgccagcag ttcgcctacc accatcttcc ctaaatcaac cgcgggaatg
     4261 ccatgaaaag ccgtcgcctg gcgggcaaaa ggcgtacgta aaccgctaac aatggcgata
     4321 cgatcgccct ggcgggtaac cagcggtaaa acctgaccca taacactccc ctgtaaaaaa
     4381 ataaataaag tggtctgacc tgatcatagt cttaaccatt tttttacatt tagccaagcg
     4441 gagaaaaggg aaattgggag ctatgacaca gtgaagacag gagaaagaaa agaaaacgcc
     4501 cctacctgtt gctggcagga gcgtacttat ccgagaggaa ttaacgcaga cccagctgga
     4561 agatcagcat ttcagcttcg caggcaaaag tgaaatcgat atccaggcgc acaccatcag
     4621 actcttcagt gaaagtcggg gagattttgc acggttcgga ttccacgcta cgggctttct
     4681 cagtcagcgc cgccagcgtt tgttctgctt ctgcgcggtt tgcaaacacg cggctgtaag
     4741 acgctgtgca gtcggagttg tccataatgg tgccaacatc catacagcag caaaccgggg
     4801 tttcatcagc actacattta ctcatcgttg atttcctctg tatgtgcacc caaggtgcca
     4861 gataaacgtt gtggatattt tacgcttccg gaaagtgctg ctccagttgt taattctgca
     4921 aaatcggata agtgaccgaa atcacactta aaaatgatct aaaacaaaat tcacccgaat
     4981 ccatgagtgc gccacctcca aattttgcca gctggatcgc gtttcttaga tcatatttga
     5041 aaaaagatag aaacatactt gcaacattcc agctggtccg acctatactc tcgccactgg
     5101 tctgatttct aagatgtacc tcagacccta cacttcgcgc tcctgttaca gcacgtaaca
     5161 tagtttgtat aaaaataaat cattgaggtt atggtcatga gccagaaaac cctgtttaca
     5221 aagtctgctc tcgcagtcgc agtggcactt atctccaccc aggcctggtc ggcaggcttt
     5281 cagttaaacg aattttcttc ctctggcctg ggccgggctt attcagggga aggcgcaatt
     5341 gccgatgatg caggtaacgt cagccgtaac cccgcattga ttaccatgtt tgaccgcccg
     5401 acattttctg cgggtgcggt ttatattgac ccggatgtaa atatcagcgg aacgtctcca
     5461 tctggtcgta gcctgaaagc cgataacatc gcgcctacgg catgggttcc gaacatgcac
     5521 tttgttgcac cgattaacga ccaatttggt tggggcgctt ctattacctc taactatggc
     5581 ctggcaacag agtttaacga tacttatgca ggcggctctg tcgggggtac aaccgacctt
     5641 gaaaccatga acctgaactt aagcggtgcg tatcgcttaa ataatgcatg gagctttggt
     5701 cttggtttca acgccgtcta cgctcgcgcg aaaattgaac gtttcgcagg cgatctgggg
     5761 cagctggttg ctggtcagat tatgcaatct cctgccggga agactcctca agggcaagca
     5821 ttggcagcta ccgccaacgg tatcgacagt aataccaaaa tcgctcatct gaacggcaac
     5881 cagtggggct ttggatggaa cgccggtatc ctgtatgaac tggataaaaa taaccgctat
     5941 gcactgacct accgttctga agtgaaaatt gacttcaaag gtaactacag cagcgatctt
     6001 aatcgtgtgt ttaataacta cggtttgcca attcctaccg ccacaggtgg cgcaacgcaa
     6061 tcgggttatc tgacgctgaa cctgcctgaa atgtgggaag tgtcgggtta taaccgtgtt
     6121 gatccgcagt gggcgattca ctatagcctg gcttacacca gctggagtca gttccagcag
     6181 ctgaaagcga cctcaaccag tggcgacacg ctgttccaga aacatgaagg ctttaaagat
     6241 gcttaccgca tcgcgttggg taccacttat tactacgatg ataactggac cttccgtacc
     6301 ggtatcgcct ttgatgacag cccagttccg gcacagaatc gttctatctc cattccggac
     6361 caggaccgtt tctggctgag tgcaggtacg acttacgcgt ttaataaaga tgcttcagtc
     6421 gacgttggtg tttcttatat gcacggtcag agcgtgaaaa ttaacgaagg cccataccag
     6481 ttcgagtctg aaggtaaagc ctggctgttc ggtactaact ttaactacgc gttctgataa
     6541 cgcgttcgcc tggataaagt cacctgctat gcaggtgact ttaactcccc cacttcaccg
     6601 aaagtagttt cctcattgct tgcctcctga gttttgcaaa cagcctgatg gcagctagcc
     6661 ctttcaaatc agtaagtggt atccacagac aaacctgaag ggaaacgcat attttcaggc
     6721 gttctgctcg tccttctcaa agagtttact tttctgaatt tccaggatac tcaaccccct
     6781 ggctattgtg cgctcataca cgcaaattaa aga
//
LOCUS       DBFIFI010000220         6800 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.220, whole genome shotgun sequence.
ACCESSION   DBFIFI010000220 DBFIFI010000000
VERSION     DBFIFI010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6800
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.220"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            347..1135
                     /gene="fabI"
                     /locus_tag="N7B39_004146"
     CDS             347..1135
                     /gene="fabI"
                     /locus_tag="N7B39_004146"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HGU2424023.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            1279..2406
                     /gene="yciW"
                     /locus_tag="N7B39_004147"
     CDS             1279..2406
                     /gene="yciW"
                     /locus_tag="N7B39_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HGU2424024.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LEKETLAPFLSWLDPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSTRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESLRWRSSYEV
                     EKLPEEHTKSSTAELCQLAEILSLHPISLSLLERLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFAACMDSSNEYKKISPLLRKGENEINQWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            2474..4408
                     /gene="rnb"
                     /locus_tag="N7B39_004148"
     CDS             2474..4408
                     /gene="rnb"
                     /locus_tag="N7B39_004148"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HGU2424025.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVRKDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(4527..4589)
                     /locus_tag="N7B39_004149"
                     /pseudo
     CDS             complement(4527..4589)
                     /locus_tag="N7B39_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4643..6623
                     /gene="pdeR"
                     /locus_tag="N7B39_004150"
                     /pseudo
     CDS             4643..6623
                     /gene="pdeR"
                     /locus_tag="N7B39_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            complement(6605..6733)
                     /gene="ymiD"
                     /locus_tag="N7B39_004151"
     CDS             complement(6605..6733)
                     /gene="ymiD"
                     /locus_tag="N7B39_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HGU2424026.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTEYSAKYIYSSTYARFR"
BASE COUNT         1680 a         1730 c         1653 g         1737 t
ORIGIN      
        1 gattctgtga agatagttga caatattaag ttggggtcaa ttcatcagaa aggctgctac
       61 ttgttgtcgc cagttctgtt ttcatcatca aacttatcgg agctaatacg aatagtttca
      121 tagtcagatt ctataagata tttccccaac ctaccccttg caggcgtcaa gagtagataa
      181 cgcaaaatgc tctgtaccgc agcttctctc cggtacagaa agcgcaacta tagccaccca
      241 cagccaggtt gattataata accgtttatc tgttcgtact gtttactaaa acgacgaatc
      301 gcctgatttt caggcacaac aagcatcaac aataaggatt aaagctatgg gttttctttc
      361 cggtaagcgc attctggtaa ccggtgttgc cagcaaactg tccatcgcct acggtatcgc
      421 tcaggcgatg caccgcgaag gagctgaact ggcattcacc taccagaacg acaaactgaa
      481 aggccgcgta gaagaatttg ccgctcaatt gggttctgac atcgttctgc aatgcgatgt
      541 tgccgaagat gccagcatcg acaccatgtt cgctgaactg gggaaagttt ggccgaaatt
      601 tgacggtttc gtacactcta ttggttttgc acctggcgat cagctggatg gtgactatgt
      661 taacgccgtt actcgtgaag gcttcaaaat tgcccacgac atcagctcct acagcttcgt
      721 tgcaatggca aaagcttgcc gctccatgct gaatccgggt tctgccctgc tgaccctttc
      781 ctaccttggc gctgagcgcg ctatcccgaa ctacaacgtt atgggtctgg caaaagcgtc
      841 tctggaagcg aacgtgcgct atatggcgaa cgcgatgggt ccggaaggtg tgcgtgttaa
      901 cgccatctct gctggtccga tccgtactct ggcggcctcc ggtatcaaag acttccgcaa
      961 aatgctggct cattgcgaag ccgttacccc gattcgccgt accgttacta ttgaagatgt
     1021 gggtaactct gcggcattcc tgtgctccga tctctctgcc ggtatctccg gtgaagtggt
     1081 ccacgttgac ggcggtttca gcattgctgc aatgaacgaa ctcgaactga aataatcgtt
     1141 ctgttggtaa agatgggcgg cgttctgccg cccgttatct ctgttatacc tttctgatat
     1201 ttgttatcgc cgatccgtct ttctcccctt cccgccttgc gtcaggataa cgatttcctt
     1261 tacgaccaag gagcgcccat ggaacaacgc cacatcaccg gcaaaagcca ctggtatcat
     1321 gaaacgcaat ccagtactac ggagtatgac gttctgcctc tggtcccgga agccgcaaag
     1381 gtcagcgatc cctttctact cgacgtgatc cttgaaaaag aaacgctggc ccccttcctt
     1441 tcatggctgg accctgcgcg tgttcttgca gtggagttgt tccctgacca gcttaccgtg
     1501 acccgttcac agaccttcac cgcttatgaa cgcttgtcga cggccctgac ggttgctcag
     1561 gtttgcggcg tccagcggtt atgtaactac tattcggcgc gacttacgcc gctccccggg
     1621 cctgattcca ccagggaaag taatcatcgg ttggcacaaa tcacgcaata tgcccgccaa
     1681 ctggctagct cgccttctat tatcgacaac cgatcgcgcc agcatctgaa tgacgtcggt
     1741 cttactgcct gggactgtgt gatcattaac caaatcattg gttttattgg ctttcaggcg
     1801 cggacaattg cgacatttca ggcttatctc gggcatccgg tacgctggtt acccgggctg
     1861 gagatacaaa actacgccga cgcgtcactg tttgctgatg aatcattacg ctggcgaagc
     1921 agctatgagg tggaaaaact acctgaagaa cacacaaaaa gttcaactgc agaactttgc
     1981 caactggccg aaatactctc tctccaccct atttcacttt cccttctcga aaggttgtta
     2041 aacagcacac gggttaatac acagccggat aatcagcttg cggcgttgtt atgcgcgcgg
     2101 ataaatggca gtcctgcttg ttttgccgcc tgtatggatt catcaaatga atataaaaaa
     2161 atcagccccc ttctgcgcaa gggcgaaaat gaaattaacc aatgggctga ccgtcattct
     2221 gttgagcgcg ctaccgttca ggcgatacaa tggctgaccc gagcacccga tcgctttagc
     2281 gccgcccagt tcagcccttt actcgaacac gaaaaatcat caacgcagat tattaatctg
     2341 ctggtatgga gcgggctgtg tggctggata aatcgcttaa aaatcgcgtt gggtgagaca
     2401 tattaacctt gccgcgtcag acagattcgc gtaaaactgt cagccgctct aatggccacc
     2461 aaaatagaca attatgtttc aggacaaccc gctgctagcg cagcttaaac agcaactgca
     2521 ttcccagacg ccacgcgctg aaggggtggt aaaagccaca gaaaaaggtt ttggcttcct
     2581 ggaagtcgac gcgcaaaaaa gttatttcat tccgccgccg cagatgaaaa aagtcatgca
     2641 tggcgaccga attatcgcgg tgatccacag tgaaaaagaa cgtgaatccg cagagccaga
     2701 agaactggtt gaaccgtttc tgactcgttt cgtgggtaaa gttcagggca aaaatgaccg
     2761 tctggccatc gttcctgatc atccactctt aaaagacgcc attccttgcc gcgcagcccg
     2821 tggtctgaac cacgagttta aagaaggcga ctgggcagtt gccgaaatgc gccgtcatcc
     2881 gctgaaaggc gatcgttctt tctatgctga actgacacaa tacatcactt ttggtgacga
     2941 ccactttgta ccgtggtggg ttacccttgc gcgccataat ctggaaaaag aagcaccaga
     3001 cggcgtcgct accgaaatgc tcgatgaagg tctggttcgt aaagatctga ccgcgctgga
     3061 ttttgtcacc atcgacagtg ccagcacaga agatatggat gacgcccttt tcgctaaggc
     3121 gttgccggat gacaaacttc agctgattgt ggcgattgcc gatccaaccg cgtggattgc
     3181 tgaaggcagc aagctggaca aagccgcgaa aattcgcgca ttcaccaact atctgcctgg
     3241 cttcaacatc cctatgctgc ctcgcgagct ttctgacgat ctctgctcac tgcgcgccaa
     3301 tgaagtccgc ccggtactgg catgccgcat gacgctctcc gctgatggca ccattgaaga
     3361 taatatcgaa ttctttgccg ccaccatcga atccaaagcg aagctggtgt atgaccaggt
     3421 ttctgactgg ctggagaata ccggtgactg gcagcctgaa agtgaagcaa ttgccgaaca
     3481 agtccgtttg ctagcgcaaa tttgccaacg ccgcggcgag tggcgtcata accacgcact
     3541 ggtgtttaaa gatcgcccgg attaccgctt tattctcggt gaaaaaggtg aagtgctgga
     3601 tatcgtcgcc gagcctcgtc gcattgccaa ccgtatcgtc gaagaagcga tgattgccgc
     3661 taacatttgt gcagctcgcg tactgcgcga taagctcggt tttggtatct ataacgtgca
     3721 tatgggcttt gatccggcga atgccgacgc gctggcagcg ttgctgaaaa cgcacggtct
     3781 gcatgtcgat gccgaagaag tgctcacgct ggacggtttc tgcaaactgc gtcgtgaact
     3841 ggatgcgcaa ccaactggtt tcctcgacag ccgcattcgt cgcttccagt catttgctga
     3901 aattagcact gaacccggtc ctcactttgg cctcggtctg gaagcatacg ccacctggac
     3961 ttcgccgatc cgtaaatatg gcgacatgat caaccaccgt ctgctgaaag cggttatcaa
     4021 aggcgaaact gcgacgcgtc cacaggatga aatcactgtc caaatggccg agcgtcgccg
     4081 tctcaatcgg atggcagaac gtgatgttgg tgactggtta tacgcacgct tcctgaaaga
     4141 caaagccggg accgacaccc gtttcgcagc ggaaattgtc gatatcagcc gtggcggcat
     4201 gcgtgttcgt ttggttgata acggcgctat cgcctttatt cctgcacctt tcttacacgc
     4261 tgtgcgcgat gaactggttt gcagccagga aaacggcacc gtacaaatta aaggtgaaac
     4321 ggtttataaa gtaactgacg ttattgacgt caccattgcc gaagtccgca tggaaacccg
     4381 cagcattatt gcgcgcccgg tcgcgtaatc tcctttcacg gcccattcct tatgaatggg
     4441 ccgtttattt ccccgctcta ccttcatcat atcccggcag ttttttaact gactttcgtt
     4501 tgaaaactgg cgtttttcca aaactagatt acaaataaat atataaaaaa aacaatgtgt
     4561 ttagccgccg ttaatttcat gtaagtcatg ttgttccttt tatttaaccg cggactccgc
     4621 tgttaaccgg aggatatgca tcatgaaaac cgttagggag tccacaacgt tgtacaactt
     4681 tctcggatcg cacaatccat actggcggtt gacggaaagc agcgatgttt tgcgcttttc
     4741 taccaccgaa accacagaac ctgaacgtac gttgcagtta tctgccgaac aggctgctcg
     4801 catcagggaa atgacggtca tcacctccag cctgatgatg agtctgaccg tcgatgagag
     4861 cgatctttct gtgcatctgg taggacgaaa aatcaataaa cgggaatggg ctggcaacgc
     4921 gtctgcatgg catgacacac cagcagttgc tcgtgattta tcacacggac tttcctttgc
     4981 tgagcaggta gtttctgaag cacattccgc aatagtgatt ctcgacagcc gggggaatat
     5041 ccaacgcttc aatcggttat gtgaagatta cacagggttg aacgaacacg acgtcattgg
     5101 ccaaagcgtg tttaaactgt ttatgagccg tcgtgaagct gcggcatcca ggcgcaataa
     5161 ccgtgtattt tttcgaagcg gcaatgcata tgaagtcgaa ctgtggatac caacacgtaa
     5221 aggccagcgg ctgtttctgt ttcgcaataa atttgtccac agcggcagtg gcaaaaacga
     5281 gattttttta atctgttccg gcaccgacat taccgaagag cgccgcgctc aggaacgact
     5341 gcgtattctg gcaaataccg acagtatcac cggactgccg aatcgtaacg caatgcagga
     5401 gttaatcgat cacgctatta atcaggcaga taacaataaa gttggggtcg tgtatcttga
     5461 tttggataat tttaaaaagg tcaacgacgc ctatgggcat ttatttggtg accagttatt
     5521 acgcgacgtg tcattggcta ttttaagctg tctcgaacat gaccaggtgt tggcgcgtcc
     5581 aggtggcgat gagtttctgg tactggcatc caacacctca caaagcgcgc tggaagcaat
     5641 ggcatcacga attttgaccc gcttacggct cccctttcgc attggtttaa ttgaagttta
     5701 taccagctgt tcagtaggta ttgcactctc tcccgaacat ggttcggaca gcgcggctat
     5761 tattcgtcac gccgacacag caatgtacac agcaaaggaa ggcggacgag gacaattttg
     5821 cgtttttacc ccagaaatga atcaacgggt atttgaatat ctctggctgg ataccaactt
     5881 gcgtaaagca ctggaaaacg atcagttggt tattcactat caacccaaaa tcacctggcg
     5941 tggcgaagtg cgcagtctgg aagcactagt acgttggcag tcacctgaac gtgggttgat
     6001 tccaccgttg gacttcattt cctacgcaga agagtcaggg ctaattgtgc ctttaggccg
     6061 ttgggtgatt ctcgatgtcg tacgccaggt ggcgaagtgg cgggataaag gcattaacct
     6121 gcgagtggcg gtaaacattt ctgcacgtca gctcgccgat caaaccattt tcaccgccct
     6181 gaaacaggtt ctccaggaac tcaattttga atactattga tgttgaattg acagagagtt
     6241 gtctgattga gaatgatgaa ctggcactgt ctgttattca acaatttagc caactaggtg
     6301 cgcaagtgca tctggatgat tttggtaccg cctactcttc actttcgcaa ctggcgcgct
     6361 ttccgatcga tgccatcaaa cttgaccagg tttttgttcg agatattcac aaacaacctg
     6421 tctcgcagtc actggttcgg gcaatcgtcg ctgtggccca ggcattgaat cttcaggtga
     6481 tcgccgaagg tgtagagagt gcaaaggaag atgctttttt aaccaagaat gggatcaatg
     6541 agcggcaagg atttttgttt gccaaaccga tgcccgccgt cgccttcgaa cgctggtata
     6601 aacgctatct gaagcgcgca taagtagatg aatatatata cttagcgcta tattccgtca
     6661 ttatatatcg tattcccgga tgataattag ccgttgctgt taaacacttc tcttttagcg
     6721 gcaatttttg cattattcat tttccccctt ttgctataag gatgtccttg atgtctgaat
     6781 ttgatgccca gcgcgttgcc
//
LOCUS       DBFIFI010000221         6764 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.221, whole genome shotgun sequence.
ACCESSION   DBFIFI010000221 DBFIFI010000000
VERSION     DBFIFI010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6764
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.221"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            36..1031
                     /locus_tag="N7B39_004152"
     CDS             36..1031
                     /locus_tag="N7B39_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGU2424027.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNDHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGSVTRIPCPLLIRCST"
     gene            complement(1039..2472)
                     /gene="cscA"
                     /locus_tag="N7B39_004153"
     CDS             complement(1039..2472)
                     /gene="cscA"
                     /locus_tag="N7B39_004153"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HGU2424028.1"
                     /translation="MTQSRLHAAQNALAKLHERRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDENDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFVAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSISPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVVQHGALWQLG"
     gene            2688..3602
                     /locus_tag="N7B39_004154"
     CDS             2688..3602
                     /locus_tag="N7B39_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HGU2424029.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGT
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTEIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDRDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVDCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELE"
     gene            3674..4921
                     /locus_tag="N7B39_004155"
     CDS             3674..4921
                     /locus_tag="N7B39_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HGU2424030.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(5001..5168)
                     /locus_tag="N7B39_004156"
     CDS             complement(5001..5168)
                     /locus_tag="N7B39_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001316510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HGU2424031.1"
                     /translation="MLHSAMHSLMNSQARDTRDRSQNDDNVLEISEPCIRSTFERLYQ
                     NMNEVFGLGDY"
     gene            complement(5519..6145)
                     /locus_tag="N7B39_004157"
     CDS             complement(5519..6145)
                     /locus_tag="N7B39_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HGU2424032.1"
                     /translation="MNVRIYCRASTEGQHADRALTSLREFSKSKGWQIAGEYIENASG
                     AKLERVELMRLLSEAQSGDLLLVEAIDRLSRLEHSAWVELKDTLNRKGLIIVSMDLPT
                     SWQMVEMAGNDLTSGILRAVNAMLIDILATMARQDYETRRKRQQQGIERAKSEGIYIG
                     RAKNQEAREIVREMLEQGVKPELIMKAAGISRATYYRIKNELLIVKSE"
     gene            6448..6624
                     /locus_tag="N7B39_004158"
     CDS             6448..6624
                     /locus_tag="N7B39_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424033.1"
                     /translation="MEKDLKELREYLLLSPVYSEINDCLASLSMEDVTNEEFAERLGI
                     APDWMDAIDTSQLA"
BASE COUNT         1602 a         1549 c         1652 g         1961 t
ORIGIN      
        1 gctacagtgc ccgttttacg gcaaacggct tgggtatggc ttcattaaag gatgtcgcac
       61 gcctggcggg agtgtcgatg atgacagtct cccgggtgat gcataatgca gaatctgtgc
      121 gtcctgcaac gcgtgaccgc gtattgcagg caatccagac cctgaattat gttcctgatc
      181 tttccgcccg taagatgcgc gctcaaggac gtaagccgtc gactctcgcc gtgctggcgc
      241 aggacactgc taccactcct ttctctgttg atattctgct tgccattgag caaaccgcca
      301 gcgagttcgg ctggaatagt tttttaatca atattttttc tgaagatgac gctgcccgcg
      361 cggcacgtca gctgcttgcc caccgtccgg atggcattat ctatactaca atggggctgc
      421 gacatatcac gctacctgag tctctgtatg gtgaaaatat tgtattggcg aactgtgtgg
      481 cggatgaccc agcgttaccc agttatatcc ctgatgatta cactgcacaa tatgaatcaa
      541 cacagcattt gctcgcggcg ggctatcgtc aaccgttatg cttctggcta ccggaaagtg
      601 cgttggcaac agggtatcgt cggcagggat ttgagcaggc ctggcgtgat gctggacgag
      661 atctggctga ggtgaaacaa tttcacatgg caacaggtga tgatcactac accgatctcg
      721 caagtttact caatgaccac ttcaaatcgg gcaaaccaga ttttgatgtt ctgatatgtg
      781 gtaacgatcg cgcagccttt gtggcttatc aggttctcct ggctaagggg gtacgtatcc
      841 cgcaggatgt cgccgtaatg ggctttgata atctggttgg cgtcgggcat ctgtttttac
      901 cgccgctgac cacaattcag cttccacatg acattatcgg gcgggaagct gcattgcata
      961 ttattgaagg tcgtgaaggg ggaagtgtga cgcggatccc ttgcccgctg ttgatccgtt
     1021 gttccacctg atattatgtt aacccagttg ccagagtgct ccatgttgca ccacagccac
     1081 tccgtgggag gcataaagcg acagttcccg ttcttctggc tgcggataga ttcgactact
     1141 catcaccgct tccccgtcgt taataaatac ttccacggat gatgtatcga taaatatcct
     1201 tagggcgagc gtgtcacgct gcgggagggg aatactacgg tagccgtcta aattctcgtg
     1261 tgggtaatac cgccacaaaa caagtcgctc agattggtta tcaatataca gccgcattcc
     1321 agtgccgagc tgtaatccgt aatgttcggc atcactgttc ttcagcgccc actgcaactg
     1381 aatctcaact gcttgcgcgt tttcctgcaa aacatattta ttgctgattg tgcggggaga
     1441 gatagattga tgctgctggc gtaacgactc agcttcgtgt accgggcgtt gtagaagttt
     1501 gccattgctc tctgatagct cgcgcgccag cgtcatgcag cctgcccagc cttcacgttt
     1561 tgagggcatt ggcgattccc acatatccat ccagccgata acaatacgcc gaccatcctt
     1621 cgctacaaag ctttgtggtg cataaaagtc atgcccgtta tcaagttcag taaaatgccc
     1681 ggattgtgca aaaagtcgtc ctggcgacca cattccgggt attacgccac tttgaaagcg
     1741 atttcggtaa ctgtatccct cggcattcat tccctgcggg gaaaacatca gataatgctg
     1801 atcgccaagg ctgaaaaagt ccggacattc ccacatatag ctttcacccg catcagcgtg
     1861 ggccagtacg cgatcgaaag tccattcacg taatgaactg ccgcgataaa gcaggatctg
     1921 cccagtgttg cctgggtctt tcgccccgac taccatccac catgtgtcgg cttcacgcca
     1981 cactttagga tcgcggaagt gcatgattcc ttctggtgga gtgaggatca caccctgttt
     2041 ctcgaaatga ataccatccc gactggtagc cagacattgt acttcgcgaa ttgcatcgtc
     2101 attacctgca ccatcgagcc agacgtgtcc ggtgtagata agtgagagga caccattgtc
     2161 atcgacagca ctacctgaaa aacacccatc tttgtcattc tcgtctcctg gcgctagcgc
     2221 aataggctca tgctgccagt ggatcatatc gtcgctggtg gcatgtcccc agtgcattgg
     2281 cccccagtgt tcactcattg ggtgatgttg ataaaacgcg tgataacgat cgttaaacca
     2341 gatcaggccg tttggatcgt tcatccaccc ggcaggaggc gcgaggtgaa aatggggata
     2401 gaaagtgtta cctcggcgct cgtgaagttt tgctagtgcg ttttgcgccg catgcaatcg
     2461 agattgcgtc atttttatca tcctggttaa gcaaatttgg tgaattgtta acgttaactt
     2521 ttataaaaat aaagtccctt actttcataa atgcgatgaa tatcacaaat gttaacgtta
     2581 actatgacat tttgtgatcg aatatgcatg ttttagtaaa tccatgacga ttttgcgaaa
     2641 aagaggttta tcactatgcg taagtcagat gaatttaagg gaaaaaaatg tcagccaaag
     2701 tatgggtttt aggggatgcg gtcgtagatc tcttgccaga atcagacggg cggctactgc
     2761 cttgtcctgg cggcgcgcca gctaacgttg cggtgggaat cgccagatta ggcggaacaa
     2821 gtgggtttat aggtcgggtg ggggatgatc cttttggtgc attaatgcaa agaacgctgc
     2881 taactgaggg agtcgatatc acgtatctga agcaagatga atggcaccgg acatccacgg
     2941 tgcttgtcga tctgaacgat caaggggaac gttcatttac gtttatggtc cgccccagtg
     3001 ccgatctttt tttagagacg acagacttgc cctgctggcg acatggcgaa tggttacatc
     3061 tctgttcaat tgcgttgtct gccgagcctt cgcgtaccag cgcatttact gcgatgacgg
     3121 agatccggca tgccggaggt tttgtcagct tcgatcccaa tattcgtgaa gatctatggc
     3181 aagacgagca tttgctccgc ttgtgtttgc ggcaggcgct acaactggcg gatgtcgtca
     3241 agctctcgga agaagaatgg cgacttatca gtggaaaaac acagaacgat cgggatatat
     3301 gcgccctggc aaaagagtat gagatcgcca tgctgttggt gactaaaggt gcagaagggg
     3361 tggtggtctg ttatcgagga caagttcacc attttgctgg aatgtctgtg gattgtgtcg
     3421 atagcacggg ggcgggagat gcgttcgttg ccgggttact cacaggtctg tcctctacgg
     3481 gattatctac agatgagaga gaaatgcgac gaattatcga tctcgctcaa cgttgcggag
     3541 cgcttgcagt aacggcgaaa ggggcaatga cagcgctgcc atgtcgacaa gaactggaat
     3601 agtgagaagt aaacggcgaa gtcgctctta tctctaaata ggacgtgaat tttttaacga
     3661 caggcaggta attatggcac tgaatattcc attcagaaat gcgtactatc gttttgcatc
     3721 cagttactca tttctctttt ttatttcctg gtcgctgtgg tggtcgttat acgctatttg
     3781 gctgaaagga catctagggt tgacagggac ggaattaggt acactttatt cggtcaacca
     3841 gtttaccagc attctattta tgatgttcta cggcatcgtt caggataaac tcggtctgaa
     3901 gaaaccgctc atctggtgta tgagtttcat cctggtcttg accggaccgt ttatgattta
     3961 cgtttatgaa ccgttactgc aaagcaattt ttctgtaggt ctaattctgg gggcgctctt
     4021 ttttggcctg gggtatctgg cgggatgcgg tttgcttgac agcttcaccg aaaaaatggc
     4081 gcgaaatttt catttcgaat atggaacagc gcgcgcctgg ggatcttttg gctatgctat
     4141 tggcgcgttc tttgccggca tattttttag tatcagtccc catatcaact tctggttggt
     4201 ctcgctattt ggcgctgtat ttatgatgat caacatgtgt tttaaagata aggatcacca
     4261 gtgcgtagcg gcggatgcgg gaggggtaaa aaaagaggat tttatcgcag ttttcaagga
     4321 tcgaaacttc tgggttttcg tcatatttat tgttgggacg tggtctttct ataacatttt
     4381 tgatcaacaa ctttttcctg tcttttatgc aggtttattc gaatcacacg atgtaggaac
     4441 gcgcctgtat ggttatctca actcattcca ggtggtactc gaagcgctgt gcatggcgat
     4501 tattccgttc tttgtgaatc gggtagggcc aaaaaatgca ttacttatcg gtgttgtgat
     4561 tatggcgttg cgtatccttt cctgcgcgct gttcgttaac ccctggatta tttcattagt
     4621 gaagctgtta catgccattg aggttccact ttgtgtcata tccgtcttca aatacagcgt
     4681 ggcaaacttt gataagcgcc tgtcgtcgac gatctttctg attggttttc aaattgccag
     4741 ttcgcttggg attgtgctgc tttcaacgcc gactgggata ctctttgacc acgcaggcta
     4801 ccagacagtt ttcttcgcaa tttcgggtat tgtctgcctg atgttgctat ttggcatttt
     4861 cttcctgagt aaaaaacgcg agcaaatagt tatggaaacg cctgtacctt cagcaatata
     4921 gacgtaaact ttttccggtt gttgtcgata tctccatatc cttcaaccgg aaaataataa
     4981 tactaaaatg cttagccctg ctaataatcg cctaatccaa acacctcatt catgttctgg
     5041 tacagtcgct caaatgtact ccggatacac ggttcgctga tttccaggac attgtcgtca
     5101 ttctgcgacc tgtcccgtgt atcacgggcc tgcgaattca tcaaggaatg cattgcggag
     5161 tgaagtatcg agtcacgccg tatttcgcta tcaggattct gtgtgatggt tacatcgccc
     5221 ggccctgggc tgtttagtca tcagcgcttt ctgacagtgc tgagatttca acctgttgca
     5281 gtaaaaatga gtagatatag ggcaagtgtg ctgccaaacc catcgtttac gggatgaagg
     5341 tagatttggt ttgaagggta tctggtgtcc ctgcaggaat cgaaaaatta gctaacaatt
     5401 tgaattgtag tttatttctc tttcgatttt ggtgagtgcc ccaataggtg cccccaagcg
     5461 ttgtgatcag aaatgctggt ttttacctga tgaaacaagc ctgacgacct ttcaaatttt
     5521 attctgattt tacgatcaat agctcgtttt ttatccggta gtaagtcgct cggctgattc
     5581 ctgctgcctt catgatgagt tcaggtttta ctccttgctc caacatctct ctaacgatct
     5641 ctcgggcttc ctgatttttc gccctcccaa tgtatatgcc ttcgctttta gctcgttcaa
     5701 tgccttgctg ttggcgttta cggcgtgttt cgtaatcctg ccgtgccatt gtggcaagga
     5761 tatcaatcaa catggcattt acggctctca ggatgccgct ggttagatca ttgcctgcca
     5821 tctcgaccat ctgccagctc gttggtaaat ccattgatac gatgattaac ccttttcggt
     5881 tgagcgtatc tttcagttcg acccatgcac tatgttctag gcgactcaga cgatctatag
     5941 cttccactag caacagatcg ccggactggg cttcagacaa tagccgcata agctctacgc
     6001 gctcaagctt cgccccgcta gcattttcta tatactctcc tgcaatctgc cagccttttg
     6061 attttgaaaa ttcacgtagg ctggtaagtg ctcgatcagc gtgttgcccc tcagttgatg
     6121 ctcggcagta aattctaacg ttcattgcat tgtctcaaaa gggttgtgaa tataagtgca
     6181 aatgatacat aatgcagttt gtgtagtcaa aatgttccta tacaacccta ttgagacttt
     6241 gtatcacctt gtcattacgg tatacccaat tgagacaatg ataacggctg gtgtttaagg
     6301 tgatatattg agctagccga aagaaacagc ccgacaccgt ttttaccgta tgagataatt
     6361 tgttgtgctg gtggggttgc ttgccagtac ttggaatggt acaaatctgt accattgttc
     6421 gacggtctat taacaaggag tgttaagatg gaaaaagacc tgaaagagtt gcgggaatac
     6481 ctattacttt cccccgtcta ttctgaaatt aacgattgtc tggctagtct ctcaatggaa
     6541 gacgtgacca atgaagagtt cgcggaacgt ttgggaattg ctcctgattg gatggatgct
     6601 atagatacta gccaacttgc ttaagaaagg atgcgccatt gctgggcgcg tttctcattt
     6661 ctcccgtagc actacgcata ccttctaata aatttcacca tcaaaatcag tagattgata
     6721 aaatgtcact caaatctgtc ataagtatca caatttttaa acct
//
LOCUS       DBFIFI010000222         6736 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.222, whole genome shotgun sequence.
ACCESSION   DBFIFI010000222 DBFIFI010000000
VERSION     DBFIFI010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6736
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.222"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(532..849)
                     /gene="yehK"
                     /locus_tag="N7B39_004159"
     CDS             complement(532..849)
                     /gene="yehK"
                     /locus_tag="N7B39_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YehK"
                     /protein_id="HGU2424034.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRSTFHQRDREDADPLINDALIYIRDECIDERKLRGESPETVIAIFNRELQNIFNQ
                     EIE"
     gene            complement(911..4540)
                     /gene="yehI"
                     /locus_tag="N7B39_004160"
     CDS             complement(911..4540)
                     /gene="yehI"
                     /locus_tag="N7B39_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein YehI"
                     /protein_id="HGU2424035.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRRWPGEPVSVITGSLIQ
                     TLGDELLQQAGQKENITWNYDKCSLEWQSAIQQAINLTGEHKPSIPALTMAALICIAQ
                     NDSQQLLDEIVQQEGLEYATDVVISRQCIARRYESDSLVVTLQYQDEDYGYGYGSATY
                     NDFDLRLRKHLSLAEESCWQRCADKLIAALPGIPKIRRPFIALILPEKPEIANELASL
                     ESSRSSLHSKEWLKVVATDNTAVKKLERYWGLDVFSDREASYMSQENRFGYAACASLL
                     REQGLAAVPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKEPPARPGYPIIEDKKLPAQQKAREEYWRTLLQTLMASQPQLAAE
                     VMSWLSTQARAVLKSYLSALPKPVIDGTDNSNLPEILVSPPWRSKKKMTAPRLDLARL
                     ELTPQVYWQPGERERLAATESARYFSTESLAQRMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILNGWNSPEAQLAEQALRSGHIEALINIWEND
                     NYSRYRPEKSVWNLYLLAQLPREMALTFWLRINEKKHLFAGEDYFLSILGLDALPGLL
                     LAFSHRPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTATALIPL
                     VFTKSSDKSEAALLALRLLYEHGHGELLQTVANRWQRTDVWPALEHLLKQGPMEIYPA
                     RIPKAPDFWHPQMWSRPRLITNNQPVTDDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQGKSARA
                     VSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRLV
                     PTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQLKAPEA
                     LARLKGLKKDATQVSKNLLPRLEAALRTTRRWSLADFHSLFVNHPFTRLVTQRLIWGV
                     YPANEPRRLLNAFRVAAEGGFCNAQDEPIDLPADALIGIAHPLEMTAEMRSEFAQLFA
                     DYEIMPPFRQLARRTVLLTPDESTSNSLTRWEGKSATVGQLMGMRYKGWESGYEDAFV
                     YDLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVYRDNKSVTFAELDVFNLSEALSAP
                     DVIFH"
     gene            complement(4550..6736)
                     /locus_tag="N7B39_004161"
                     /pseudo
     CDS             complement(4550..6736)
                     /locus_tag="N7B39_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860777.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
BASE COUNT         1618 a         1646 c         1680 g         1792 t
ORIGIN      
        1 gatcgcagaa aataaaaaaa cctgctggtg aggcaggttc ttttatttac tacagtgacg
       61 ctaacttgct gattgtgcaa catttcgtac caacgaataa attataacca tatccacact
      121 ccattgcaat tcagtgagtg agatttataa tcctgcccgt tgggaggttg tgccggatcg
      181 gaccaatttt aagtcctggc tgccggacgg gtggtgccgc aggcggtgcc ctgatccgga
      241 gagcaacgtt tcgtaccaac atacgtaaac cagtatcgac aggcagtgta agggtaaggt
      301 gctggtgttg cgctccttaa taaggagagc ggatggacac gctgacacaa aagttaaccg
      361 tgctcattgc cgtactggag ttattagtgg ctctgttacg gttgattgat ttgttgaagt
      421 aacgggcaaa ttgaaacgca tctaaggggg aataccgttt ccccctttaa gaaagctata
      481 ctcgtcatac ttcaagttgc atgtgctgcg actttaatta tctagagtat attattctat
      541 ttcttggttg aatatattct gtagttcacg attaaaaatt gctattacag tttcaggtga
      601 ttcacctcgt aatttccgct catcaataca ctcatctctt atataaatca acgcatcatt
      661 tattagcggg tcagcatctt ctcgatcacg ttgatgaaat gttgaacgta gccggaatcg
      721 ctccagtcga tagacggaac ctgtaaataa ttcagagcat ttatggatct caagtctgaa
      781 ggaaaacgga tcttcaggta cagggacctc ataatcgtaa atagcaacca gctctttttg
      841 cacgatcatc gctttccccg ctggtaagtc gtgttgataa cgggcagttg catcactgcc
      901 cgctgtgttt ttaatggaaa atgacgtcag gtgcgcttaa cgcctcactc aaattaaaca
      961 catcaagttc ggcaaaagtg acggatttat tgtcacggta cactcgaaga gaacggaagc
     1021 tcattaacgc tttgctatca acattgtagt ggttaaaacc gggtgaaaac ttaaggacca
     1081 gccggtactc acccaggtca tagacaaatg cgtcctcata acctgactcc cagcctttgt
     1141 atcgcattcc cataagttgc ccaacggtag cggatttacc ttcccagcga gtcaggctgt
     1201 tactggttga ctcatcaggt gtgagcagca ccgtgcggcg cgccaactgg cgaaaaggcg
     1261 gcataatttc gtaatcggca aaaagctgtg caaattcact gcgcatttct gctgtcattt
     1321 ctaacgggtg ggcaatgcca atcagagcgt ccgcaggcag gtcaattggc tcatcttgcg
     1381 cattgcagaa ccccccctct gcggccacac gaaaggcgtt gagtaaacga cgcggttcat
     1441 ttgccggata aaccccccat attaatcgct gggtaaccag acgggtaaag ggatgattaa
     1501 caaacagaga atgaaaatct gccagcgacc agcgtcgggt ggtacgtagg gcagcttcaa
     1561 gacgcgggag caggtttttg ctcacctgag tagcatcttt ttttagccct tttagtcggg
     1621 ccagtgcctc gggcgctttc agttgatcgt catcggcgcg caaccgggga acgcttttct
     1681 ggcgaacgtt ttgctgatca aagataaccg ggttgaggtt ttcatcgaag cgaacggtaa
     1741 actgccgggg accaaaatca aaactcaacg cctgcggatc atcaaggccc agggttggga
     1801 ctaatctgtc cgctagctct tcctggctta gcccgcgatt ttctgcgact acctgaagaa
     1861 attccgccgc gttatcacgt aaggggcggg atttagcccg ttgcgatata tgatgcagct
     1921 gtatcagcgc catatcatta ttcatcaggg taaggatgtt caggccgctg acagcacggg
     1981 cagatttgcc ttgtggccag gcgaggatct gtgttgttag atcccgtgcc gtgctttcgt
     2041 caccaaagag acttaacgcc agaaatgccc agttgtcttt tgcgggggca ccagcttgtt
     2101 gccacgcagt gaagagatcc caggcaaaag ctgccagcgt ttgtggctgg cagaacgttt
     2161 tcagttgttc cagcccgcta taaaaacgtc ccccctgggt aaagcgcagc atttcgccga
     2221 taatttccag agcgtcatcg gtaacaggtt gattattagt gataaggcgc ggcctggacc
     2281 acatttgcgg atgccagaaa tcaggggctt ttggaatgcg tgccgggtaa atttccatgg
     2341 gaccctgttt cagtaaatgc tccagggcag gccatacatc tgtacgctgc caacgattgg
     2401 ctacagtttg tagcaattcg ccatgcccat gttcgtaaag taaacgcagg gcaagtaatg
     2461 cagcttcgct tttatcgcta gatttggtga agacaagagg gataagtgca gtagccgtat
     2521 gttccggcca ttgtaaaatc cactggcgag ccagatcacg ctgcgccgcg aaacgtcgcc
     2581 agacgcgggc aacgggcagg gccagttctg ttgcgccgaa atttaaaatt aacggaaatg
     2641 tttcttttgg acgatgtgaa aaagccaaca gcagacctgg tagcgcatcc aatccgagga
     2701 tactgagaaa atagtcctct cccgcgaaca gatgcttttt ctcattgata cgcagccaga
     2761 aagtcaaagc catctcacgc ggcaactgtg ccaataaata caggttccag acactctttt
     2821 ccggacgata acgtgagtag ttgtcatttt cccatatgtt aattaacgct tctatgtgac
     2881 cgctgcggag cgcctgttct gctaattgtg cttccgggga gttccagcca ttcaggatct
     2941 cttcaacccg cccctggtag taatgccact gaacaatgag tgaattgcgt gcagcatcca
     3001 gttttccagg gagtatataa ttcaaaaaca gccatacatc atccccaaaa cccagttcct
     3061 gtaatacaac tcgcccactt ttttgttcca tgcgttgcgc aagagattcc gtgctgaaat
     3121 aacgggcaga ctcagtggcg gcaagcctct ctcgttcgcc tggttgccag taaacttgcg
     3181 gagttaattc gagccgtgcc aaatcaagac gtggagctgt catttttttc ttactacgcc
     3241 acggtggtga aacgaggatt tcaggcagat tgctgttatc ggtgccatca ataaccggtt
     3301 ttggcagtgc cgataaataa ctcttcagca ctgccctggc ttgagtactt aaccacgaca
     3361 tcacttctgc tgccagttgt ggctgcgatg ccatcagcgt ttgtaacagc gtacgccagt
     3421 attcttctcg agctttttgc tgtgcaggca gctttttgtc ttcgatgatt ggataaccag
     3481 ggcgggctgg tggttctttt aacgccagca gttctgccag tgcggcgagc gtcgcatggg
     3541 ggaagttttt actgtattta gccacacgtt gcaggctggg tttgttttta tcagcaacta
     3601 acagcaaggt gcggatgact tgcggatggt taatttgtac cagcagactg ccgcaatctt
     3661 ctttatgagc atacattgcc aggcgcggaa cggctgccag tccttgttcg cgcaaaaggg
     3721 aggcgcaggc cgcgtagccg aagcggtttt cttgtgacat gtagctggct tcgcgatcgc
     3781 taaatacgtc caggccccag tatcgttcga gtttttttac cgccgtatta tcagtagcaa
     3841 cgacctttaa ccactccttt gaatggagac ttgaccggga gctttcaaga ctggcgagtt
     3901 cattggctat ttcaggtttt tccgggagga tcagcgcaat aaaaggacgg cgaattttgg
     3961 gtattcctgg cagtgcggca atgagtttat cggcacaacg ctgccagcaa ctttcctctg
     4021 ccagcgacag atgcttacgc aaacggagat cgaaatcgtt ataggtcgcg gagccataac
     4081 cgtagccata gtcctcgtcc tgatactgca aagtgacgac cagtgagtca ctctcataac
     4141 gccgcgcaat gcactgacgc gatatcacta catccgtcgc atattccagc ccctcttgtt
     4201 gcacgatttc atcaagtaac tgctggctgt cattttgcgc gatacagatc agagccgcca
     4261 tggtaagggc tgggattgac ggtttatgct cgccagtcaa attgattgcc tgctggatgg
     4321 cggactgcca ctccagtgag catttatcat aattccaggt gatattttcc ttctggcctg
     4381 cttgttgtag taattcatct cccaatgtct ggatgagact tccagtgata acggacactg
     4441 gttcgcccgg ccagcgacga tgactgagcg gcgttttgcc ttttttatat ttcagttcca
     4501 gttgggcgtt atccgccagc cacggtaatt ccttgtccat gctctttcct tattcaaaca
     4561 gggcttcaat atcgttaatt agctcgctgg cggtgatggc atcgagtacg gagaactgcg
     4621 cttcctgtgt tgaattactc ccccagccat agctttctgc gttgccttcc catagccaca
     4681 gcttgcttaa gatttgctcg gcactcagtt cggctggcga catgcctacc gcaaaacctt
     4741 catcgatttc catgattaac gaccagcggc ccagaggttt gatcatccag ccgatccagc
     4801 ctccatcctg cggggtgccc tttatccagc ctttattcgc cagccccatg acccgaccgc
     4861 tcgggcattt tctgcctgcc cagcgggtca gttcgctggc gttgcgctcg gcttctgtca
     4921 gggcgtaact gttacggtca agctgacgga acggtggtag cagttcatag tcggcaaaaa
     4981 gctgaccaaa ggcggtagca tccgtcggtg atatttccag aacgtgagga atgccaacag
     5041 agatatcgcc ttccggcagg gtgaaaagat catcgtcagc ggtgctgtag ctgttatctt
     5101 ccgccacgcg gaagcacgtc agtagctggt tttcggtgct ataaacgccc caaatcagac
     5161 ggcgggttaa gtggcgtacc agtggatgtt caaccaggaa gagctgaaag ttttctggcg
     5221 accagcggcg acgcaggcac atggcggatt ccaaccttgc tacctgctgg gcggcgacgg
     5281 tacgcgcatc ttttttcagc agtttgtagc ggttaacagc atcgttcgcc tgtgattcat
     5341 catcgctttt gtttggtttg ggcaggtctt tcagacggct gccagaaaca tcacgcacaa
     5401 acggttttaa ggtttcatca aagctgacgg tgaactggcg tgagccgaaa tccagcagca
     5461 acgaaccgtt atcatccaga ccgagatccg gtgctaaccg atcttcaagc tctgccaccg
     5521 tgagttcgcg gctttcggca atgccggcaa ttttttcttt tgcccgctcc tgtaatgctt
     5581 tgaatttcag tttctgggca ataccgttaa gctgcatcag ggcgatatca ctaccgatag
     5641 cggcgagaat atccaaccca acggtggcgc gtttatgctg ggattcacca ggccaggcgc
     5701 gtattaatgg cgtcagtttg cgggcggtgt catcgttacc aagaacgcct aacgcagtga
     5761 acgcccaact ctctttcgac ggtgcgcccg cagtcagcca ggcggtaaac agatcccagg
     5821 caaattcagc cagtgagtcg gcggtacagg catctttcac ctgcaataat cccggataca
     5881 gggcctcttc ctgagggaag cggagcattt caccgaggtg ctggagtgcg ctatctggca
     5941 gtaccttgct gctatttttg agtttcgggc gagtccataa tggaggttga taaaacaacg
     6001 gtaaaggggg gattttcgcc gggtatttac ccacaggact gttgtcgtta tttttatttt
     6061 gagtttccca gggcggcgag actagtacca ttggtagaat ttctgagttt gcaaaatctt
     6121 cattgcgact attcagcaat tgctgacatt gttgcaataa atttgccgct tgcgacgata
     6181 atccgatgac taatttttcg acaaataaag gatttgcatt aagtaatgaa accagcaatg
     6241 tatgtttgcg tttatcatct ttttttacca atgcttcggc taatgccact atcgttgcag
     6301 aaggaaaatt ttcggcactt tttattcgta atggccaacg gatgattaat acatttcagt
     6361 acgtcaccgc aattatcttc ttcagcatag ggagtcaggc gagccagtgc cgctatacct
     6421 tgctcccgta ataccgtagc tgaccaactg gcaccatagg tgttaaatac atcaatgtat
     6481 tgatagttct ccagtatttc gagcgtgaat ggatctgtcg ctaccagttt caacatttca
     6541 agttgtggaa catctgcgac ctggtgtaaa cgatgtacga tctcatgggc cacttcgggt
     6601 atttctggca ttaaaatggc aattaacggt tggcgataga gtgatatatc tggtaacgca
     6661 acgagtaatt ttgcaatgca attttgccat acttcttctt ctgctagtga taaatgttta
     6721 cgcagacgta aactaa
//
LOCUS       DBFIFI010000223         6731 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.223, whole genome shotgun sequence.
ACCESSION   DBFIFI010000223 DBFIFI010000000
VERSION     DBFIFI010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.223"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            1..512
                     /gene="ydjO"
                     /locus_tag="N7B39_004162"
                     /pseudo
     CDS             1..512
                     /gene="ydjO"
                     /locus_tag="N7B39_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjO"
     gene            complement(516..1907)
                     /gene="tcyP"
                     /locus_tag="N7B39_004163"
     CDS             complement(516..1907)
                     /gene="tcyP"
                     /locus_tag="N7B39_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HGU2424036.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHH"
     gene            complement(2040..2630)
                     /gene="ydjM"
                     /locus_tag="N7B39_004164"
     CDS             complement(2040..2630)
                     /gene="ydjM"
                     /locus_tag="N7B39_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HGU2424037.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(2793..3461)
                     /gene="hxpB"
                     /locus_tag="N7B39_004165"
     CDS             complement(2793..3461)
                     /gene="hxpB"
                     /locus_tag="N7B39_004165"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HGU2424038.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3608..4144
                     /gene="yniB"
                     /locus_tag="N7B39_004166"
     CDS             3608..4144
                     /gene="yniB"
                     /locus_tag="N7B39_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HGU2424039.1"
                     /translation="MTYQQAGRIAVLKRIMGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGTRMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(4185..5045)
                     /gene="yniA"
                     /locus_tag="N7B39_004167"
     CDS             complement(4185..5045)
                     /gene="yniA"
                     /locus_tag="N7B39_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HGU2424040.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(5151..5441)
                     /gene="ghoS"
                     /locus_tag="N7B39_004168"
     CDS             complement(5151..5441)
                     /gene="ghoS"
                     /locus_tag="N7B39_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HGU2424041.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5542..6471)
                     /gene="pfkB"
                     /locus_tag="N7B39_004169"
     CDS             complement(5542..6471)
                     /gene="pfkB"
                     /locus_tag="N7B39_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HGU2424042.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
BASE COUNT         1772 a         1639 c         1622 g         1698 t
ORIGIN      
        1 agtgaaattc ggataagagg tataaataaa gattttattg gaatggatgt cacttctgta
       61 tttcgcccag aaaaaatgac caatttgatc agtataaaaa acaggttggt tattcactat
      121 ttaaggacta tatactatga acaatattat atacatcctg tcgggagcat ttttgctgcg
      181 attcaaaaaa atgagagtct cctaaagttt ccttcgataa tatcaatgct taatatcaat
      241 cttcttttta atccactaaa tcttccggga atgggaagtg gtatacttga agatattatg
      301 agtattccag atagttcact tagaaaacgt ctcgggtatg aggttttaag cttctcgcta
      361 caggcacatt cattgagcca ggaatgtatt gataagttgg acattttttc gctgacgatc
      421 tgtttaaata tgagtcagta tgcatcgccg cgatggaaca tttaaaaagt aaggcaacgg
      481 cccctttaca aaacggaccg ttgccagcat aagaattaat ggtgtgccag ttcggcgtcg
      541 tcttcgctat ccagaatggc tttatcggtt tgcttcagcc actggctggt cagcgtgcca
      601 gctgtcatcg agccactaac gtttagcgcc gtacggccca tgtcgataag cggttcaacg
      661 gagattaaca gcgccaccag ggttactggc aggcccatcg caggcagcac aatcagtgcg
      721 gcgaaagttg caccaccacc gacaccggca acgcctgcgg agctaacggt aacaataccg
      781 accagcgtcg caatccacat cgggtccagc gggttaatgc caaccgtagg cgcaaccatc
      841 accgccagca ttgccggata caaaccggcg cagccgttct gaccaatggt cgcaccgaaa
      901 gaggcggcga aactggcgat ggattcagga acaccgagtc gacgcgtttg tgcttccaca
      961 ttcagtggga tagacgcagc actggaacgg ctggtaaagg caaacgtcag cacaggccat
     1021 accttacgga agtacttcag cggactcacg ccattaatgc ccagcagaat gccatgcact
     1081 gcaaacataa tcagcagacc gaggtaggac gcgacaacga aacttcccag tttgatgatg
     1141 tcttgcaggt tagaacctgc aaccactttg gtcattagtg ccagaacgcc gtaaggggtc
     1201 aactgcatga ccaggcgaac tagtttcatc acccagcttt gcagggtatc gatagcggtt
     1261 aagacgcgtt cacctttcgg cgcatcatcc ttcagcagtt tcagcgcagc tacaccgagg
     1321 aatgcggcaa aaattaccac gctgataatt gacgtcggat tggctccggt cagatcggca
     1381 aacgggtttt tcgggataaa ggacaagacc agctgcggaa cgctcaggtc agagacttta
     1441 ccaacatagt tactttcgat ggcgttcaga cgtgcagttt ctgcaccacc ctgaaccaga
     1501 ccttcagccg tcaaaccaaa caggttggtt accagcacac cgaccagcgc cgcaatcagc
     1561 gtggtaaaca aaagcgtacc aatggtcaga aaactgattt tgcctaactg agatgcgtta
     1621 tgcagacggg caaccgcgct cagaatagag gcgaagacta acggcataac gatcatttgc
     1681 agcagttgaa catagccgtt accaacaatg ttaaaccact gtacagaatc tttaagtacc
     1741 tggctgtcag aaccataaat ggtatgcagg gcaaggccaa aaactacacc catcaccaga
     1801 cccaccagca cttttttcgc cagactccac tgtttatggc gggtctgagc cagcgcaaac
     1861 agcagtacaa cgaacaccac gatgttcgca attaatggaa agttcatccc cgttctcctg
     1921 atcttattat ttctatcact gcgtagcagt catagtgttg gcgcaagatt aacagaactg
     1981 tgagctggct cttatattca aatggaattg cttattacag aaaggcgatt tttgcctttt
     2041 tagtattcaa cctgatgctt aataagccga tgaaactgga tctgcaaggt attgatcatt
     2101 tgcgatgacc aacgaacagc gctgttttcg ggtaatgaat gaggcatcca taccgaccag
     2161 acaaataatg ccatgcagat aaaacgttcc agttggttac ccttttgggg aaccaggata
     2221 ggcaagcgga aacgccagcg gcatggccag agcaggggaa caccggcggg tgtcagcata
     2281 tcggcaagta tgtggcttaa ataacccagc accattcctt gtagcgcatc agccggaatg
     2341 aaccagcttt caggaacctt aaggtaaaag gttgccagca gcgcaaatac cgccagcaga
     2401 ctgtgggtaa aaccacgatg cccaaaagcg cgggcgatcg gttttgatat ccactttaat
     2461 cgctgcccaa gaaacgactt tgggtgatcg atgtccggta acaaacacgt caggattgcg
     2521 gaagggacaa tatgccacca gtcaccctgt gccagcacgg gcgtcagctc ggcatttttg
     2581 gcaaataccg cacaagcaat agaaaagaga aggtgacctt ccgccgtcat gataaaactc
     2641 actaaactgt cgatacgtac agtataggat ttttatacag tgcacggaag tggtgacggt
     2701 gtaactcttt ctcttaaatt gacgattaat ccgtcagttg aatgcaaatt cagataaaga
     2761 accaggggca gcgtgatcac tgcccctggt cattaaccga gaaggtcttt tgcggtgagt
     2821 tctgtcagcg atgagagttt gacgtttgct aatacaaaac gtggatcatt ttgcgcttct
     2881 ggcgcaggaa cgacgatgga acgcatgcgg gctgctttag aggcgatcat gccatttacc
     2941 gaatcctcca gcgctacgca ggtcagtggg tcaacgccca gttttgctgc gcagtcgaga
     3001 tatacttgcg gatgcggctt gctgtaaggc agtttttcgg ccgaggcgag ggcatcgaaa
     3061 ctgtcgcgta agtcaaacat ggtcaacact ttttccagca tatgtagtgg tgacgcggag
     3121 gccagtccca ccaataaacc ttgttctttg cataacgcaa cggcttcgcg cacgcctggt
     3181 aataatggac gtgtctcttc aaccagtgaa atggcacggg caataacccg ttctactact
     3241 tcctgacggc ttggcccatt ccacggttgc cgggcgtacc aaagatcgac caccatatcg
     3301 atgcgtaaac ctaaggtgtc cggcagctcg ttacgacggg agatatccac ccccaggctt
     3361 gccatcacat ccagttcggc tcgatcccat aaaggttctg agtcgataag taatccatcc
     3421 atatcaaaaa ttgcagcaag aatctgacgc ggggttgaca ttacaacctc tcctttagag
     3481 cattatgtgt aaggtattaa gccgtgctta tgattaagcg cctatcatat cgcctttaaa
     3541 gaatgggcga aaacggatat ctacaggtaa actaagtgaa acgacgcgtc ttaatcaagg
     3601 agaactcatg acgtatcaac aagctggacg cattgctgtt ttgaaaagga ttatgggttg
     3661 ggtgattttt atacctgcat tgatctctac attgatttct ttgctgaaat ttatgaatac
     3721 ccggcaggaa aaccaggaag gcattaatgc ggtcatgctc gactttactc atgtcatgat
     3781 cgatatgatg caggcgaata cgcctttttt gaatctgttc tggtataact ccccgacgcc
     3841 caattttaat ggtggcgtga acgttatgtt ctgggtgatt ttcatcctga tttttgttgg
     3901 actggcgttg caggattccg gtacccggat gagtcgccag gcgcgcttct tgcgggaagg
     3961 tgttgaagat caactcattc tggaaaaagc caaaggggaa gaggggctaa cgcgtgaaca
     4021 aattgagtca cgtattgttg ttccgcacca tactattttc ctgcagtttt tctcactgta
     4081 tatcctgccg gttatttgta ttgctgcagg ttatgtgttc ttttctctgc ttgggtttat
     4141 ttaatcaaaa ggccgcacgg agcggccatt atcttcaacc catatcacgc agctaataat
     4201 ctatccagtg actgctgagc aataaccaaa tgctgaccgc caaataatct tgcacgattt
     4261 aacagcgtgt agagttggta aaccggttga cgttcaagga aatcggcagg tagcggggat
     4321 actgactgat agccgtcata gatttgtggc ggctgttcag tatgcagcgg taacatcgcc
     4381 aggtcgcact ctcggtcacc ccagtagcag gccgggtcga aaatgtacgg gccatccgga
     4441 cccagcgcac agttgccgga ccataaatcg ccgtgcaaca gagaaggctg cggttgatgt
     4501 gaggcgagac gctgctgaat atgctcgacg atggcatcga tattgccgaa agcgatccct
     4561 ttttctgctg ccagttccaa ctgccagcca atccgttgtt cagcaaaaaa cgttgaccag
     4621 cgacgttgcc aggtgttggg ctgtggagtt gtggagagcg agttatcaaa atcgagtcca
     4681 aattgtggtt ggtcgctcca ttgatgtaaa cgcgcaattt gctgaccaag aataaatgcg
     4741 ctatgcgcat ccagcggacg aggtgggaga taatccatca ccagaaaact gtagtcacgg
     4801 tcagcgccaa ctgcccaaac cttaggcacg gtgacggttt tactacgcga cagtaactcc
     4861 agttggtcgg cttcggcggt aaaaccggga agcagttccc tttcatcaca tttgacgaaa
     4921 aagtcatgtc ctgcatagcg caaatgccat gcggcatgga cttctccgcc aggcagttca
     4981 ttacgcagtt cgatttcgcc ttcacctaac tgctcgctca aaagacgact gattgcctgc
     5041 cacatgatgt ctctcccatg ttgtctgcca gatcataagg ttagcgcaag aatgcggtgt
     5101 aaaaacacga gctaacgcac accacagact gattattgcc caaaaaccat ttattttctg
     5161 ttgctattcc attcctccca ggtcgtgatg gtgatttcag gatctttgcc ggttgcactt
     5221 tgggtaagcc ccgaaaccag ttctctaatt tcttcttcac tttgggtact gataagtcca
     5281 aaagtgttag tccccagctc atggatattt ccctcatcat ccgtcatggt gagcaaaaaa
     5341 ccgtcgcgag tcaggtaatt attcaactcg ttaatttcag tcaatgtatc ctcatgcaac
     5401 atgacggtta tgacataacg gacaagatcg ccactggcca tagttcaccc ctttgttatc
     5461 acatgacttt tgagcatagt cggagaaacg cgttgccgac aggttggtga tgattccccc
     5521 aatgctgggg gaatgttttt gttagcggga aaggtaagcg taaatttttt gcgtatcgtc
     5581 atgggagcac agacgtgttc cctgattgag tgtggctgca ctccccgcag ctacgccaaa
     5641 acgaaccatc tcttcaagag aggcattttc tgccagtttc agtgtcatcg cgccgaccat
     5701 gctatcacca gcgccaacgg tactctggct tttcaccggt ggtggcacca cctgaatgca
     5761 gttttcacta tcaacaccca gcgctccttg tggacccagg gaaacgacaa cccgtttggc
     5821 cttgccgcta ttaacgattt cctgcgcggc tttgcggaca tcgtctggct gggtgagttc
     5881 gcgattcacc agcgcactga gttctttttg gttaggctta accaactcga tgttaccaat
     5941 cgccagtgct gcacttaacg cttcgccaga actgtcgatg atgcagcgga tcccttggtt
     6001 ttgcgcagcg gaaatcagtt gggttaattt ttccagcttc acacctggcg gcaggcttcc
     6061 gcttatgacc aggatggccc cggattcaat ttccagaact tgctcttcaa gctggcgaaa
     6121 ctcatcttca tttaatgccg cgcctggcat aacaaaacga tactgctcac cgcttgcttc
     6181 cacatgtacg tgtaaattct gccgggtcca gtctttggct tctacagtag cgacggggac
     6241 attttcatcc gccaacagtg aaaccaggtg ttcgccggtc gcgccacccg ccgggaagat
     6301 cgctgtggca ctgcctccaa gatgggcaat ggcgcgggcg acgttgatgc cgccgccccc
     6361 gggttcgaac accggtgcgg tacagcgcag ttttccttcg ggataaattt gcggggtaat
     6421 tgttgcgcta tcgagagagg gcgcaagtgt caacgtatag atacgtacca tcatttcctc
     6481 ctataggctg atttcagtct ggcaccgaat cagcggaaag tgaagaaatt aacaatatga
     6541 tttattggag ctttatttaa aattttgcag ataaatatat ataaataaaa atctgttttc
     6601 gccacatgct tattcaaaaa atgaaataag aatacattga taagttattc gcgccttttt
     6661 ataattcgtt acctttccag ggacgctaat ctttgatcct taagaaagtt tccgggaccg
     6721 cgatggtctc t
//
LOCUS       DBFIFI010000224         6604 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.224, whole genome shotgun sequence.
ACCESSION   DBFIFI010000224 DBFIFI010000000
VERSION     DBFIFI010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6604
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.224"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            82..1668
                     /gene="pdeC"
                     /locus_tag="N7B39_004170"
     CDS             82..1668
                     /gene="pdeC"
                     /locus_tag="N7B39_004170"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HGU2424043.1"
                     /translation="MNHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIVPVNGYTIVSADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVPRNGYLYATVY
                     STKRPIAAIVATSYQRLIAHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWPGEQGQVMNPAEFIPLAEKEGMIEQV
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            complement(1671..1994)
                     /gene="soxS"
                     /locus_tag="N7B39_004171"
     CDS             complement(1671..1994)
                     /gene="soxS"
                     /locus_tag="N7B39_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HGU2424044.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            2080..2544
                     /gene="soxR"
                     /locus_tag="N7B39_004172"
     CDS             2080..2544
                     /gene="soxR"
                     /locus_tag="N7B39_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HGU2424045.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            3090..4439
                     /gene="ghxP"
                     /locus_tag="N7B39_004173"
                     /gene_synonym="yjcD"
     CDS             3090..4439
                     /gene="ghxP"
                     /locus_tag="N7B39_004173"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HGU2424046.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            4590..6239
                     /locus_tag="N7B39_004174"
     CDS             4590..6239
                     /locus_tag="N7B39_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HGU2424047.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(6393..>6604)
                     /locus_tag="N7B39_004175"
     CDS             complement(6393..>6604)
                     /locus_tag="N7B39_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HGU2424048.1"
                     /translation="VNFDEASVQGMGISILRGEENIPINSDTLVTLQKFFEEDCTSHT
                     GMSQTENNTHEVAMKITADIMQHAD"
BASE COUNT         1451 a         1559 c         1770 g         1824 t
ORIGIN      
        1 cgtaagattt atctaaagac gtcggtagcc agggtttcac cttgcaatgg ccgggtataa
       61 acaggcagga aattgatagc aatgaatcat cgtgcacgac accaattact ggcgttgccg
      121 ggcattatct ttttagttct ctttcccatc attctttcgc tatggattgc cttcctttgg
      181 gcaaaatcag aagtgaataa tcagctccga acctttgctc aactggcgtt ggataaatcc
      241 gagctggtca ttcgccaggc agatttagtg agcgatgcag ctgaacgcta tcaggggcaa
      301 gtttgcactc cagcccatca aaagcgaatg ttgaatatta ttcgtggcta tctttatatt
      361 aatgaattga tctatgcccg tgataatcat tttttatgct catcgctgat agtgcctgta
      421 aacggctata cgattgtatc ggccgattat aagcgtgaac ctaatgtttc tatctattat
      481 taccgcgata cgcctttttt ctctggctat aaaatgacct atatgcagcg gggaaattat
      541 gtggcggtta tcaaccctct cttctggagt gaagtgatgt ctgatgaccc gacattgcaa
      601 tggggtgtgt atgatacggt gacgaaaacc tttttctcgt taagcaaaga ggcctcagca
      661 gcaacgtttt ctccgctgat tcatttgaag gatttaaccg taccaagaaa tggctattta
      721 tatgcgacag tttattcgac aaaacgccca attgcagcca ttgttgcgac ttcatatcaa
      781 cgtcttatag cccattttta taatcatctt atttttgcgt tgcccgccgg tattttgggg
      841 agtcttgttc tgctattact ctggctacgt attcgacaaa actatttgtc tcccaaacgc
      901 aaattgcaac gcgccctcga aaaacatcag ctatgccttt attaccagcc aataattgat
      961 atcaaaacag aaaaatgtat cggcgctgaa gcgttgttac gttggcctgg tgagcagggg
     1021 caagtaatga atccggcaga gtttattcct ctggcagaaa aggaggggat gatagaacag
     1081 gtaactgatt atgttattga taatgtcttc cgcgatctgg gcgcgtacct tgcaacacat
     1141 gctgatcgct atgtttctat taatctgtcg gcctccgatt ttcatacgtc acggttgata
     1201 gcgcgaatca atcagaaaac agagcaatac gcggtgcgtc cgcagcaaat taaatttgaa
     1261 gtgactgagc atgcatttct tgatgtcgac aaaatgacgc cgattattct ggctttccgc
     1321 caggctggtt acgaagtggc aattgatgat tttggtattg gctactctaa cttgcataac
     1381 cttaaatcat tgaatgtcga tattttgaaa atcgacaaat cgtttgttga gacgctgacc
     1441 acccataaaa ccagtcattt gattgctgaa catatcatcg agctggcgca cagcctgggg
     1501 ttaaaaacga tcgctgaagg cgtcgaaact gaggagcagg ttaactggct gcgcaaacgc
     1561 ggcgtgcgct attgccaggg atggttcttt gcgaaggcga tgccgccgca ggtgtttatg
     1621 caatggatgg agcaattacc cgcgcgggag ttaacgcgcg ggcaataaaa ctacaggcgg
     1681 tggcgataat cgctgggagt gcgatcaaac tgccgacgga aaacgcggga gaaggtctgc
     1741 tgagagacat aacccaggtc cattgcgata tcaaaaatcg gacgctcggt ggtgcgcaac
     1801 tcaacggcgg ccagtaacag acggcgttga cgaatgtaat cgccaagcgt ctgatgcgtc
     1861 accgtgcgga acatccgttg caggtaccac tttgaatagc ctgatttttt tgcgacgaca
     1921 tcaatgttaa gcggctggtc aatatgctcg tcaatccatg cgataagatc ctgaataatt
     1981 ttctgatggg acataaatct gcctcttttc agtgttcagt tcgttaattc atctgttggg
     2041 gagtataatt cctcaagtta acttgaggta aagcgattta tggaaaagaa attaccccgc
     2101 attaaagcgc tgctaacccc cggcgaagtg gcgaaacgca gcggtgtggc ggtatcggcg
     2161 ctgcatttct atgaaagcaa agggttgatt accagtatcc gtaacagcgg caatcagcgg
     2221 cgttataaac gtgatgtgtt gcgatatgtc gcaattatca aaattgctca gcgtattggc
     2281 attcctctcg cgaccattgg tgaagcgttt ggcgtgttgc ccgaagggca tacgttaagt
     2341 gcgaaagagt ggaaacagct ttcgtcccaa tggcgagaag agttggatcg gcgcattcat
     2401 accttagtgg cgctgcgtga cgaactggac ggatgtattg gttgtggctg cctttcgcgc
     2461 agtgattgcc cgttgcgtaa cccgggcgac cgcttaggag aagaaggtac cggcgcacgc
     2521 ttgctggaag atgaacaaaa ctaaagcgcc acaagggcgc tttagtttgt tttccggtct
     2581 ttgtctttct ctctatcccg ctggtacaca ggagggtttc ccccgacgtc aacacacctc
     2641 attcgagcac gtggtggagg ttccggttgg tgttgatgct ttaattgtat gtcaccgacg
     2701 tttcttcgcc agtgtaaaag tatacttttt agccgcaata tttttgtcat ctcagacgat
     2761 tttttatcgc aatcctcaac ggtatacggc tcgataacgc tgcaatcttg cgcatcgacg
     2821 ataacgtttg cgcatcaatt gcctggtttt tcatcgtcaa gacaataaaa gagaaaaaag
     2881 cagcaaactt cggttgaaaa agccgctatg atcgccggat aatcgtttgc tttttttacc
     2941 acccgttttg tatgcgcgga gctaaacgtt tgcttttttg cgacgcagca aattgtcgca
     3001 aacctggagc aggaagataa cgtttcgctg gcaggggatt gtccgccacg catcttgacg
     3061 aaaattaaac tctcagggga tgttctctta tgtctacgcc atcagcgcgt accggcggtt
     3121 cactcgacgc ctggtttaaa atttcacaac gtggaagcac tgtccgtcag gaagtggttg
     3181 ccgggttaac aacgtttctg gcgatggtct actcggttat cgtcgttcca ggcatgttgg
     3241 gtaaagcggg cttcccgcct gcggcagttt tcgttgcgac ctgtctggtt gccggactcg
     3301 gttctatcgt gatgggtctg tgggctaatc tgccgttggc gattggttgc gccatctccc
     3361 tgacggcgtt taccgcattc agcctggtgc tggggcaaca tattagcgta cctgtcgcgc
     3421 tgggtgccgt gttcctgatg ggtgtgctgt ttacggtaat ttctgccacg ggtatccgta
     3481 gctggatttt gcgcaacttg ccgcacggtg tggcgcacgg cacggggatc ggtatcggtc
     3541 tgttcctgct gctcattgcc gctaacggtg tcggtctggt gattaaaaac ccgcttgatg
     3601 gtctgcccgt tgcgctgggt gatttcgcga ccttcccggt gattatgtca ctggtaggtc
     3661 tggcggtgat catcggcctg gaaaaactga aagtccctgg tggcattctg ctgaccatta
     3721 tcggtatctc aattgtcggt ttgatcttcg atcctaacgt ccatttctcc ggcgttttcg
     3781 ccatgccttc attgagcgat gaaaacggca attcactgat tggcagcctg gacattatgg
     3841 gcgcgctgaa tcctgtagtc ctgccaagcg ttctggcgct ggtgatgacg gcagtatttg
     3901 atgccaccgg aactatccgc gctgtcgccg gccaggcgaa cctgctggat aaagatgggc
     3961 agatcatcga cggcgggaaa gcactgacca ctgactccat gagcagcgtt ttctctggcc
     4021 tggtgggtgc agctccggca gcggtataca tcgagtctgc ggcgggtacg gcggcgggcg
     4081 gtaaaaccgg tttgacggct atcaccgttg gcgtgctgtt cctgctgatt ctgttcctct
     4141 ctccgctttc ttacctcgtt ccggggtatg caacggctcc ggcgctgatg tacgttggcc
     4201 tgctgatgct gagcaacgtg gcgaaaatcg actttgctga ttttgttgat gcgatggcgg
     4261 gtctggttac ggcggtattc atcgtgctga cctgtaacat cgtaacaggc atcatgatcg
     4321 gcttcgcgac tctggtgatt ggtcgtctgg tttccggtga atggcgcaag ttgaacatcg
     4381 gtacggtcgt tatcgccgtg gcgctggtga cattctatgc gggtggctgg gctatctaat
     4441 ctttcatcgg attctgaaaa cgggtggcaa tggctgcccg tttttatttt ctccgcacat
     4501 tgtgtgagct tttgcgatat tctgaaaaaa tgagaattca ggcataacgt ctttctcaga
     4561 tcataaaaaa catcgcaaac agggaacgca tggaaatctt cttcaccata ctgataatga
     4621 ccctcgtggt ctcgctgtcc ggggtggtca ctcgtgtcat gccctttcag atcccgcttc
     4681 cgcttatgca aatcgccatc ggtgcgctac tggcgtggcc gacgtttggt ttgcatgtgg
     4741 agtttgatcc tgaactcttt ttagtcttgt ttatcccgcc gttgctgttc gctgatggct
     4801 ggaaaacgcc gacccgtgaa tttctcgaac atggtcgaga gattttcggc ctcgcgctgg
     4861 cgctggtggt ggtcaccgtg gtcggcattg gcttccttat ttactgggtg gtgccgggca
     4921 ttccgctgat ccccgccttt gcgctggcgg cggtgctttc tccgaccgat gctgtggcgc
     4981 tctccgggat tgttggcgaa gggcgcatcc cgaaaaaaat catgggcatt ttgcagggcg
     5041 aagcgttgat gaatgacgcc tccggcctgg tgtcgttgaa gtttgccgtg gcagtggcga
     5101 tggggacgat gatcttcacc gtcggcggtg cgacggtcga atttatgaaa gtagccattg
     5161 gcggtattct cgccggtttt gtggtgagct ggctgtacgg tcgctcgctg cgattcctca
     5221 gccgctgggg cggtgatgaa cccgcgacgc agatcgtcct gctgttcctg ctgccattcg
     5281 cttcttatct gattgccgaa catattggcg tttccggcat cctcgctgcg gttgccgccg
     5341 ggatgaccat cacccgctcc ggtgtgatgc gccgtgcgcc gctggcaatg cgcctgcgtg
     5401 caaacagcac ctgggcgatg ctggaatttg tctttaacgg catggtgttc ctgctgttag
     5461 gtctgcagct gccgggtatt ctggagacgt cgctgatggc agcagaaatc gaccctaacg
     5521 tcgaaatctg gatgctgttt accgatatta ttctgatata tgcggcgctg atgctggtcc
     5581 gtttcggctg gctgtggacg atgaaaaagt tcagcaaccg cttcctgaag aagaagccga
     5641 tggagtttgg ttcgtggacc acacgagaaa tcctgatcgc gtctttcgcc ggggtgcgtg
     5701 gggcgatcac tttggccggt gtgctctcta tcccgctgct cttgccggat ggtaacgtct
     5761 tcccggcgcg ctatgagctg gtgtttctgg cggctggtgt cattctcttc tcgctgtttg
     5821 tcggcgtggt gatgttgcct attctgctac aacacattga agtcgcggac cattcgcaac
     5881 aattgaaaga agaacgtatt gcgcgagcgg caacggcaga agtggcgatt gtggcgatcc
     5941 agaaaatgga ggagcgtctg gcggcggata ccgaagagaa tatcgataac cagctgctta
     6001 cggaggtcag ttctcgcgtc attggtaacc tgcgtcgtcg cgccgatgga cgtaacgacg
     6061 ttgaaagttc cgtgcaggaa gagaaccttg agcgtcgctt ccgtctggcg gcattgcgtt
     6121 ctgaacgtgc tgaactgtac cacctgcgcg ccacgcggga gatcagcaac gaaacgctgc
     6181 aaaaattact gcacgatctc gatttgcttg aagcgttgct aattgaggaa aatcagtaag
     6241 ccgaaagcga cgtaaccagg cccgaggtaa tctgaaaaag gtgcaatctt ttgcaccttt
     6301 aacaggttag ctcgggctaa cattcatgat tctaaaacaa ataagttatc tctcttttaa
     6361 agtcatttca ttggctgttc ttcgacttaa tctcaatctg cgtgttgcat aatatctgca
     6421 gtaatcttca tagctacttc atgagtatta ttctcagttt gtgacatgcc agtatgagag
     6481 gtacaatctt cttcaaagaa tttctgtagt gttaccaggg tatcactatt aatgggaata
     6541 ttctcctccc cacggagaat ggaaatgccc attccctgga cgctagcctc atcgaaatta
     6601 accc
//
LOCUS       DBFIFI010000225         6556 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.225, whole genome shotgun sequence.
ACCESSION   DBFIFI010000225 DBFIFI010000000
VERSION     DBFIFI010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6556
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.225"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            271..396
                     /locus_tag="N7B39_004176"
                     /pseudo
     CDS             271..396
                     /locus_tag="N7B39_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
     gene            461..721
                     /locus_tag="N7B39_004177"
                     /pseudo
     CDS             461..721
                     /locus_tag="N7B39_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            771..1010
                     /locus_tag="N7B39_004178"
                     /pseudo
     CDS             771..1010
                     /locus_tag="N7B39_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(1058..1675)
                     /gene="ureG"
                     /locus_tag="N7B39_004179"
     CDS             complement(1058..1675)
                     /gene="ureG"
                     /locus_tag="N7B39_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HGU2424049.1"
                     /translation="MNIIKQPLRVGVGGPVGSGKTALLEALCKSMRDTWQLAVVTNDI
                     YTREDQRILTEAGALEAERIVGVETGGCPHTAIREDASMNLAAVEALSEKFGNLELIF
                     VESGGDNLSATFSPELADLTIYVIDVAEGEKIPRKGGPGITKSDFLVINKTDLAPYVG
                     ASLEVMERDTLRMRGERPWGFTNLKSGEGLQNIIAFIEEQGMLGK"
     gene            complement(1687..2361)
                     /locus_tag="N7B39_004180"
     CDS             complement(1687..2361)
                     /locus_tag="N7B39_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HGU2424050.1"
                     /translation="MPTPEKRLRLMQLASNSLPVGGYSWSQGLEWAVEAGWVEDSAAF
                     EHWQQLQMEQSFFAVDLPLLARLYRACEAGDPDSAGRWTAYLLACREPRELRDEERNR
                     GAAFTRLLVDWQPDCPAEWRKLCQQSQLTGMAWLGVRWQISVSDLALSLGYSWIESAV
                     MAGVRLVPYGQLAAQQLIMRLCARYAANMDSALATPDHAIGSATPLASIASARHETQY
                     SRLFRS"
     gene            complement(2362..2826)
                     /gene="ureE"
                     /locus_tag="N7B39_004181"
     CDS             complement(2362..2826)
                     /gene="ureE"
                     /locus_tag="N7B39_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HGU2424051.1"
                     /translation="MLYLTRRVETPAQTTASVTLPVDMRVKSRIKVTLNDGRQAGLLL
                     PRGLLLRDGDILSNENGDEFIKVIAADEAVSVVRCADPFMLAKACWHLGNRHVPLQIM
                     PGELRYHHDHVLDDMLRQFGLDVDFAHLPFEPEAGAYASKSHAHNHDQEHSH"
     gene            complement(2836..4539)
                     /gene="ureC"
                     /locus_tag="N7B39_004182"
     CDS             complement(2836..4539)
                     /gene="ureC"
                     /locus_tag="N7B39_004182"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HGU2424052.1"
                     /translation="MSNISRQAYADMFGPTTGDKIRLADTELWIEVEDDLTTYGEEVK
                     FGGGKVIRDGMGQGQMLSAGCADLVLTNALIIDYWGIVKADIGVKDGRIFAIGKAGNP
                     DIQPNVTIPIGVSTEIIAAEGRIVTAGGVDTHIHWICPQQAEEALTSGITTMIGGGTG
                     PTAGSNATTCTPGPWYIYQMLQAADSLPVNIGLLGKGNCSNPDALREQVAAGVIGLKI
                     HEDWGATPAVINCALTVADEMDVQVALHSDTLNESGFVEDTLTAIGGRTIHTFHTEGA
                     GGGHAPDIITACAHPNILPSSTNPTLPYTVNTIDEHLDMLMVCHHLDPDIAEDVAFAE
                     SRIRQETIAAEDVLHDLGAFSLTSSDSQAMGRVGEVVLRTWQVAHRMKVQRGPLPEES
                     GDNDNVRVKRYIAKYTINPALTHGIAHEVGSIEVGKLADLVLWSPAFFGVKPATIVKG
                     GMIAMAPMGDINGSIPTPQPVHYRPMFAALGSARHRCRVTFLSQAAAANGVAEQLNLH
                     STTAVVKGCRTVQKADMRHNSLLPDITVDSQTYEVRINGELITSEPADILPMAQRYFL
                     F"
     gene            complement(4532..4852)
                     /locus_tag="N7B39_004183"
     CDS             complement(4532..4852)
                     /locus_tag="N7B39_004183"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HGU2424053.1"
                     /translation="MIPGEYKVKPGYIELNIGRATCSIIVENHGDRPIQVGSHYHFAE
                     VNPALKFDRQKARGYRLNIAAGTAVRFEPGQKREVELVALSGARIVHGFRGDIMGELE
                     ANDE"
     gene            complement(4861..5163)
                     /locus_tag="N7B39_004184"
     CDS             complement(4861..5163)
                     /locus_tag="N7B39_004184"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HGU2424054.1"
                     /translation="MELTPREKDKLLLFTAALLAERRLARGLKLNYPESVALISAFIM
                     EGARDGKSVAALMEEGRHVLSREQVMEGIPEMIPDIQVEATFPDGSKLVTVHNPII"
     gene            complement(5254..5952)
                     /locus_tag="N7B39_004185"
     CDS             complement(5254..5952)
                     /locus_tag="N7B39_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HGU2424055.1"
                     /translation="MLDLRFQRLHGKTTLTTRHHVGLLTVQRPFYPEEETCHLCLLHP
                     PGGIVGGDELIINAIIDSDCHTLITMPGASKFYRSSGAQAHLQQNLTLCNNATLEWLP
                     QDSIFFPGAHATLHTVFHLSSSSTLLAWDLLCLGRPVIGETFSHGTLANRLEIWVDGS
                     PLLIERLHVANGELTCVARKPWVGTMLFYLGNETQLEDIREKLTPLENYAGATLTDGL
                     LTVRFLSDDNLRCQ"
     gene            6333..>6556
                     /locus_tag="N7B39_004186"
     CDS             6333..>6556
                     /locus_tag="N7B39_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424056.1"
                     /translation="MTEHWSGHADDNFPFSWGSDSIFMHACLQSYGESICDTVHTFVA
                     DVAPESLTGLQTVYLYTAGLLCDIVFHIEGG"
BASE COUNT         1528 a         1695 c         1731 g         1602 t
ORIGIN      
        1 aagcgagccg ttgcggaggc ttgctctctc ctttaacctc atgtgcaata tccgtaacgc
       61 gctcacataa atcatcatct gataatataa caacacccat tattttattc tgacctaaaa
      121 acttcttatg aaataaacgt ttacccgacg tatctgatcc cgctgttttt acctgtatcc
      181 tttaacatac ttgagaagcc cgctgaaaaa atataccgta aaggcggttt ctctgtcttc
      241 cataaacctg tcggataaga agggcattaa atgaaaacca aaaaagttat gatgattgca
      301 ctggtcagct ctgcgctggc tctttcagga tgtggagcca tgggcacagc gatcaaaaaa
      361 cgaaatctgg aagtaaaaac ccagatgagt gaaacctgag gtagcctgag tttaacggac
      421 actccttcct gaaatagaat ggcatcagaa ggagctaata atgagcagaa aaacccaacg
      481 ttactctaaa gagttcaaag ccgaagctgt cagaacggtt cttgaaaatc aactttcgat
      541 cagtgaaggc gcttcccgat tatctcttcc tgaaggcact ttaggacaat gggttaccgc
      601 cgccagaaaa gggctcggta ctcctggttc ccgcacggtg gctgaactgg aatctgaaat
      661 tctgcaactg cgtaaggcgt taaatgaagc tcgccttgag cgagatatat taaaaaaagc
      721 agctgtagat tcaatctgtc aatgcaacac ccctttcaat tatctctttc actgcccgcg
      781 acggtatcgg ttatcagttt gacctgctgg ctaacggtga aaccgtactg cgtgatgcat
      841 ccggcgaaaa acgcatcaag ggtgaaaaag acggtcgtat gctcatgaat gttggcctga
      901 atgcggaaat tcgcgacaac atccgcttcg gtctggagtt tgaaaaatcg gcatctggta
      961 agtacaacgt ggataacgcg gtgaacgcca acttccgtta ttccttctga taaaaacaag
     1021 gggccgcctg ccggccctga tacagaggat gttgtgatta cttacccagc atgccctgct
     1081 cttcgataaa ggcgatgatg ttttgcagac cttcaccact tttcaggtta gtgaaccccc
     1141 atgggcgttc tccgcgcata cgtaaagtat cacgctccat tacctcgagg gaggcgccca
     1201 cgtatggtgc aaggtcagtt ttattgatca cgaggaagtc ggatttggtg atccccggac
     1261 cgcctttacg cgggatcttt tctccttctg ccacatctat gacatatatg gtaaggtccg
     1321 ccagttcggg gctgaaggtc gcactcaggt tgtcgccgcc gctttccaca aagatcagct
     1381 cgagattacc aaatttctca ctcagtgctt ccacggctgc cagattcatt gatgcgtctt
     1441 cacgaatggc tgtatgaggg cagccccccg tttcgacacc gacaatgcgt tccgcttcca
     1501 gtgctccggc ctcagtgaga atgcgttgat cttctctggt gtagatgtcg ttggtgacta
     1561 cggctaattg ccaggtgtca cgcatggatt tgcagagggc ttccagaagc gcggttttac
     1621 ctgagccgac cggaccgccg acgccgacac gcagcggttg cttgattatg ttcattttat
     1681 tttctcctaa gaacggaata atcgggaata ctgggtttca tgtcgcgcgg aagcgataga
     1741 tgcaagtggc gttgctgaac caattgcgtg atccggggtt gcaagggcac tgtccatgtt
     1801 cgccgcatag cgggcacaca ggcgcataat taactgctgg gcagccagct ggccgtaggg
     1861 taccagcctg acgcccgcca tcactgcgct ttcaatccag ctgtagccaa ggctcagggc
     1921 caggtctgaa acggatattt gccagcgtac gccgagccat gccataccgg ttaactggct
     1981 ttgctggcat agtttgcgcc attcggccgg gcagtcgggc tgccagtcga ccagaagtct
     2041 ggtgaaggct gcaccgcggt tacgttcttc atcgcgtagc tcacggggtt ccctgcaggc
     2101 cagcagataa gcggtccagc gcccggcact gtcaggatcg cccgcttcac aggcccggta
     2161 tagccgggca agcagtggca ggtcgacggc aaaaaaactc tgttccatct gcaactgctg
     2221 ccagtgttca aaggcggcgc tatcttctac ccagcctgct tccactgccc actccagtcc
     2281 ctgggaccag ctgtagcctc caacagggag gctgttactg gcaagctgca tcagccgcag
     2341 acgtttttcc ggagtcggca ttcagtggct gtgctcctga tcgtgattat gggcgtgcga
     2401 cttgctggcg taagcgccgg cttccggctc aaacggaaga tgtgcaaaat caacatccag
     2461 cccaaactgg cgcagcatat catcgaggac gtgatcgtga tggtagcgca gctctccggg
     2521 cattatttgc agcggaacgt ggcgattgcc gagatgccag caggctttcg cgagcataaa
     2581 aggatctgcg cagcgcacta cagaaacggc ttcatcagcg gcgatcacct taatgaattc
     2641 atcaccgttt tcgttactga gaatatcacc atcgcgcagc agcagaccgc gtggcagcaa
     2701 cagtccagcc tgacgtccat cgttaagggt gactttaata cggcttttta cccgcatgtc
     2761 aacagggagg gtaacgctgg cagttgtctg agccggggtc tccacccgcc gggtcaaata
     2821 aagcatttgt gctccttaaa acaggaaata acgttgcgcc attggcagaa tgtccgccgg
     2881 ttcactggtt atcagttcgc cgttgatacg cacttcgtag gtttgtgaat ccacggttat
     2941 atcaggcaac aggctgttgt ggcgcatatc ggctttttgt actgtgcggc agccttttac
     3001 cacagcagtt gtgctgtgca ggttaagctg ttcagcgacg ccatttgctg ctgctgcctg
     3061 cgacaggaaa gtcacacgac agcggtgacg ggcactgccc aatgcagcga acattgggcg
     3121 atagtgcacc ggctgcggtg tggggataga gccgttgata tcacccatcg gcgccatggc
     3181 tatcattccg cctttgacga tagtcgccgg ttttacgcca aagaacgccg gggaccagag
     3241 caccagatcc gccagttttc ccacttcaat cgagccgact tcatgagcaa taccgtgggt
     3301 taatgccgga ttaatggtgt atttagcgat atagcgcttc acgcggacgt tgtcgttatc
     3361 accactttct tccggtaacg ggccgcgctg aactttcatc cggtgtgcca cctgccaggt
     3421 tcgtaacact acttctccga cgcgtcccat ggcctgcgaa tcggacgagg tgagggagaa
     3481 cgcgccaagg tcgtgcagga cgtcttccgc ggcaatggtt tcctggcgaa tgcgcgattc
     3541 ggcaaaggct acgtcctcgg cgatatccgg gtcaagatga tggcaaacca tcagcatgtc
     3601 cagatgctca tcaatagtgt tgacggtata gggtagcgtc ggattggttg aggaaggcag
     3661 aatattgggg tgcgcgcagg cggtgataat atccggagca tgaccaccac ctgcaccttc
     3721 tgtatggaag gtatggatag tgcgcccgcc gatggcagtc agagtatcct caacgaatcc
     3781 tgattcgtta agcgtatcac tgtgtagcgc aacctgaacg tccatttcgt cggctacagt
     3841 cagtgcgcag ttgattaccg caggtgtagc tccccagtct tcgtgaattt tgaggccgat
     3901 aaccccggcc gcgacctgct cacgaagcgc atccggattg gagcaattgc ctttacccag
     3961 caacccgata ttgaccggca ggctgtctgc agcctgcagc atttgataaa tgtaccatgg
     4021 tcctggggta caggttgtgg cgttagaacc cgctgtcggg ccagtaccgc caccgatcat
     4081 ggtggtaatg ccggatgtca gcgcttcttc agcctgctgt gggcagatcc agtgaatatg
     4141 cgtatcgacg ccacctgcgg taacgatcct gccttctgcg gcaataattt ccgtggatac
     4201 gccgattggg atagtgacgt tgggttgtat atcaggatta ccggctttgc cgatagcaaa
     4261 tatccttcca tctttgacgc cgatatcggc tttaacgatc ccccagtaat cgatgatcag
     4321 ggcattggtc agcaccagat cagcgcagcc ggcggagagc atttgcccct gtcccatacc
     4381 gtcgcggatt actttaccgc cgccgaattt gacctcttcg ccgtaggtag ttaaatcatc
     4441 ttcgacctcg atccacagct cagtgtctgc cagacgaatt ttatcaccgg tggtagggcc
     4501 gaacatgtca gcataggcct ggcgtgaaat attactcatc atttgcctcc agctcaccca
     4561 ttatatctcc gcggaaaccg tgcacgatac gggcaccgct cagcgccacc agttcaacct
     4621 cacgcttttg gcctggttcg aagcgcactg cagttccggc ggcgatattg agacgatagc
     4681 ctcttgcctt ctggcgatcg aacttcagtg ccgggttaac ctccgcgaag tgataatggg
     4741 aaccgacctg aattggtcgg tctccgtgat tttcaacgat aatactgcag gtagcccggc
     4801 cgatgttaag ctctatatag ccaggtttaa ccttatattc tccagggatc atctgttacc
     4861 tcagattatc ggattatgga cggtaaccag cttggagccg tccggaaagg tggcttcgac
     4921 ctggatatcg gggatcattt ctggtatgcc ttccatgacc tgctcgcgac tcaggacatg
     4981 ccgtccttct tccatcagcg cagcgacgct tttgccgtcg cgagcgccct ccattataaa
     5041 agcgctaatc agggccacgg attcgggata gttaagtttc aggccgcggg ccagacgacg
     5101 ctctgccagc agtgcagcgg taaacagtaa tagtttgtct ttttctcttg gagtcagttc
     5161 catagcgttc tcttaagtca gccagatccg gggaagtacc ggtgatttgt cagtcagttg
     5221 cggccgcata aattgccaga tatcacgcat tacctactgg caacgcagat tatcatcact
     5281 taaaaagcga actgtcagta gaccatcggt gagcgtggct ccggcgtagt tttccagtgg
     5341 tgtgagtttt tcgcgtatgt cttccagttg cgtttcattc cccaggtaga acagcatcgt
     5401 tcccacccat ggtttccggg caacgcaggt aagttctcca ttggccacgt gcagacgctc
     5461 aatcaacagc ggagaaccgt cgacccatat ctccagacgg ttagcgagtg ttccgtggct
     5521 gaaggtttct ccgatgaccg gacgtccaag gcacagaaga tcccaggcca gcagtgtact
     5581 ggaggacgac agatgaaaca cggtatgcaa cgtggcgtga gcgccgggaa aaaaaatcga
     5641 atcctgtggc aaccactcca gcgttgcgtt attgcacagc gtcaggtttt gctgaaggtg
     5701 cgcctgcgcg ccgctgctgc ggtaaaattt actggcacca ggcatggtga tcagcgtatg
     5761 gcaatcagag tcaattattg cgttgatgat gagttcgtca ccgccgacga taccaccggg
     5821 tggatgaagc agacagaggt ggcaggtttc ttcttcggga taaaaaggac gctgaacagt
     5881 gagcagaccg acatgatgac gggtggtgag cgtggttttc ccgtgcagac gctgaaaacg
     5941 gagatccagc atggcctgcc agcctttttt attttgtggt aacgcggtac tgagcaatct
     6001 tacgcccctg ataaataaag ataaaacgac agtaaagagc cggatttcct tgttatataa
     6061 aagatgatgc ctttccagtg agttttcgtc tgaataatac gaatgtttgt tttcgtcatg
     6121 aagcgttccg gctcccgggg ctatacatcg ccagaaaagc aatgcatatt gccgggatta
     6181 tccagagaat caggcagaaa gatccgacaa tgggctgtga ttatgccatc tggctcagaa
     6241 tgatatcaag gtgttatggg gataacttgc tgatacaata gcagtatgac agattcatta
     6301 atgcggaaac tgctcactga aacagagtgt tcatgactga gcactggtcg ggtcatgcag
     6361 atgataattt ccccttttct tggggaagcg acagtatttt catgcatgcc tgtcttcagt
     6421 catacgggga gagcatctgt gatacagtcc atacatttgt tgcggacgtc gctcctgagt
     6481 cactgactgg tctgcaaaca gtttatctgt acacggcagg gcttctgtgt gatatcgttt
     6541 ttcatatcga gggggg
//
LOCUS       DBFIFI010000226         6542 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.226, whole genome shotgun sequence.
ACCESSION   DBFIFI010000226 DBFIFI010000000
VERSION     DBFIFI010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6542
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.226"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..365
                     /gene="paa"
                     /locus_tag="N7B39_004187"
     CDS             <1..365
                     /gene="paa"
                     /locus_tag="N7B39_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AcfC family adhesin Paa"
                     /protein_id="HGU2424057.1"
                     /translation="TSNTSGTGVWEDIAGRKGNIETVAAIRKNIILYAPNSGTARKAL
                     ENQPGADVWITWADWAASNPEIGDVVEIAPDYVIWRDMNITVRQDANDETRRFAEWLQ
                     TDEAAPAFKKYGWTRKGT"
     gene            701..856
                     /gene="hokD"
                     /locus_tag="N7B39_004188"
     CDS             701..856
                     /gene="hokD"
                     /locus_tag="N7B39_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HGU2424058.1"
                     /translation="MKQQKAMLIALIVICLIVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1077..1334
                     /locus_tag="N7B39_004189"
     CDS             1077..1334
                     /locus_tag="N7B39_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424059.1"
                     /translation="MRIEELREIFSEDGLYTVRVEKGAIVSHCRIKCLQSQQRKSGAA
                     LIHFVDGLVTDGFILRANEFVTSLPSLKEAGIKAGFSAFED"
     gene            1404..1682
                     /locus_tag="N7B39_004190"
     CDS             1404..1682
                     /locus_tag="N7B39_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424060.1"
                     /translation="MAQNSRLHNSDNSAVFASRHGRRSHAFKSDWFRHAPCTEEQAEW
                     LIQNYRRRGYEFRKALSLDYRHWIIYVRLPYSERPPRPSRTFQQRIWR"
     gene            1684..2730
                     /locus_tag="N7B39_004191"
     CDS             1684..2730
                     /locus_tag="N7B39_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGU2424061.1"
                     /translation="MRVLLRPVPVPELGVVVLKPGRESMQVFHNPRVLVEPEPKSMRG
                     LPSGIVPAVRQPLAEDKSLLPFFSNERVIRAVGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATIILKQR
                     TKNLAPLPHAHQQNPPQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYTRWVKTQPCA
                     CCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLI
                     FRFLDHAFATGVLG"
     gene            2743..3102
                     /locus_tag="N7B39_004192"
     CDS             2743..3102
                     /locus_tag="N7B39_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HGU2424062.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVVLIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYKIESE"
     gene            3111..3641
                     /locus_tag="N7B39_004193"
     CDS             3111..3641
                     /locus_tag="N7B39_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HGU2424063.1"
                     /translation="MIYPEITGKSGEHLRLNTLEAVWIQGKLRMWGRWSYIGGGKSGN
                     MFNRLLVSKKLTKTAVNEVLRSMKKSGLEKPELEAFFRDMTRGKQKSWLSHCTDTEAL
                     IIDRVISEVLGEYPGLINILRQRYEGRGMSKRKMAECLNRTHPEWCFSTCEKRIAGWL
                     AVAEHMLYVPMHDSFR"
     gene            3883..4080
                     /locus_tag="N7B39_004194"
     CDS             3883..4080
                     /locus_tag="N7B39_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HGU2424064.1"
                     /translation="MLKQQDMTETARVVFNELSVTDPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            4231..5289
                     /locus_tag="N7B39_004195"
     CDS             4231..5289
                     /locus_tag="N7B39_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HGU2424065.1"
                     /translation="MLNTVKISSCELINADCLEFMRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWAGDEDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDTEIMMRERFNVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYEAKGRTLKQHVMA
                     PLIAYFRDARAVLGITAKQIADATGKKNMVSHWFSAGQWQLPNESDYLKLQALFARVA
                     EEKHQRGELEKPHHQLVDTYASLNRQYAELQSEYKHLRRYFGVTVQVPYTDVWTYKPV
                     QYYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTVKAAMALGRRATGVELET
                     ERFEQTVREVQDLIIRNG"
     gene            5330..5405
                     /locus_tag="N7B39_004196"
     tRNA            5330..5405
                     /locus_tag="N7B39_004196"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5363..5365,aa:Ile,seq:cat)
     gene            5486..5562
                     /locus_tag="N7B39_004197"
     tRNA            5486..5562
                     /locus_tag="N7B39_004197"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5520..5522,aa:Arg,seq:tct)
     gene            6086..6178
                     /locus_tag="N7B39_004198"
                     /pseudo
     CDS             6086..6178
                     /locus_tag="N7B39_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001455460.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
     gene            6169..6542
                     /locus_tag="N7B39_004199"
                     /pseudo
     CDS             6169..6542
                     /locus_tag="N7B39_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="9-O-acetyl-N-acetylneuraminate esterase"
BASE COUNT         1663 a         1517 c         1826 g         1536 t
ORIGIN      
        1 gtaccagtaa tacatcaggc actggcgtct gggaagatat tgccggacgt aaagggaata
       61 tagaaactgt cgccgcaatc cgaaaaaata ttattttata tgcgcccaat agcggaactg
      121 cacgtaaggc tcttgagaat cagcctggag cagatgtctg gataacctgg gctgactggg
      181 cagccagtaa tccagaaatt ggtgatgtcg tggaaatagc gccagactac gtgatatggc
      241 gtgatatgaa cattacagta cgtcaggatg caaatgatga aacccgtcga tttgcagaat
      301 ggctacaaac cgatgaagcg gcgcctgcat tcaaaaaata tggctggacc aggaaaggca
      361 cttgacatcc tcgtccttca ggacgtggat tctttttccg gatgccgcgc cagcggcatg
      421 taggggcagc tcacaaaacg gaaaaaattg tacgctaagc ctcgccaggt gaactgaatt
      481 cattccgata tgggaattcc catatcgggc gaaaacggtt tgctgtaacg gcagagttaa
      541 gtaggattgc tgcgggtgct tgaggctatc tgcctcgggc atgaacacca acggcagata
      601 gataaaagcc ccacccgact ataaatcgaa gtgaggcccc tatatgctcg tcacatatag
      661 attgcctctt acggaccgaa aggtcaagga gaagcaggct atgaagcagc aaaaggcgat
      721 gttaatcgcc ctgatcgtca tctgtttaat cgtcatagtg acggcactgg taacgaggaa
      781 agacctctgc gaggtacgaa tccgaaccgg ccagacggag gtcgctgtct tcacagctta
      841 cgaacctgag gagtaagaga ccaggcgggg gagaaatccc tcgccacctc tgatgtgtca
      901 ggcatcctca acgcacccgc acttaacccg cttcggcggg tttttgtttt tattttcaac
      961 gcgtttgaag ttccggacgg cgccggaata gaatcaaaaa tacttaagta gcgcgcaggg
     1021 agaagaggga tggaccccga acaggggagt gctatttatc tggaaggatt ctgttgatga
     1081 gaatcgaaga attacgtgaa atttttagtg aagatggcct ctatactgtg cgcgttgaga
     1141 agggcgctat tgtcagccac tgccgtatta aatgtttaca gtctcaacaa aggaagagtg
     1201 gagctgcgtt aattcatttt gtggatgggc ttgtgacgga tggttttatt ttgcgtgcaa
     1261 atgaatttgt cacatcgttg ccgtctctga aagaagctgg gattaaggct ggtttttctg
     1321 cttttgaaga ttagtgaatt catctacaat tcagcgcagg gctgaacccc tgttgagtaa
     1381 cactgtgcca ccggagaaag ccgatggcgc aaaattccag actacacaat tctgataatt
     1441 cagccgtctt tgccagcagg cacgggcggc gttctcatgc attcaaatct gactggttcc
     1501 ggcacgcccc atgcactgaa gaacaggccg aatggctgat tcagaactac cgcagacgtg
     1561 ggtatgagtt taggaaagcc ctcagcctcg attatcgtca ctggataatc tacgtcaggc
     1621 tgccgtactc cgagcgccca ccgcgtccgt cccgcacatt ccagcaacgc atctggaggt
     1681 aacgtgcggg tattacttcg acctgttccg gtaccggaac ttggggtggt ggtccttaaa
     1741 ccaggccgtg aatccatgca ggtattccat aaccctcgag tgcttgtgga gccggagccg
     1801 aaaagcatgc gcggtctgcc gtccggcatc gtccctgccg ttcgccagcc gctggcggag
     1861 gataaatcat tactgccgtt tttcagcaat gagcgtgtga ttcgtgctgt tggtggcgct
     1921 ggtgcactgt ctgactggct gttgcgtcat gtcaaatcct gccagtggcc tcatggtgac
     1981 tatcatcaca gtgaaaccgt tattcaccgt tatggtaccg gcgcaatggt gttgtgctgg
     2041 cactgcgaca accagctgcg tgaccagaca tccgaatcac tcgagcaact tgctcatcaa
     2101 aacctgtcag catggatgat tgacgtcatc ggtcacgcaa taagcggtac gcaggagcgt
     2161 gaattatctc tggctgaatt atcctggtgg gcggtccgca atcaggtggc ggacgcgcta
     2221 ccggaagcgg tattacgtcg ttcgctgggg ttgcgtgcgg aaaaaatccg ctcaatgtac
     2281 cgtgaaagcg acatcgtacc gggagagcag accgccacca tcatactgaa gcagcgcaca
     2341 aaaaatcttg cgccgctgcc tcacgcccac cagcaaaacc caccacagga aaagacggtg
     2401 gtcagcattg ccgttgatcc ggagtctccg gaatccttca tgaaacgacc taaacgtcgc
     2461 cgctgggtaa atgagaaata cacacgctgg gtaaagacac agccgtgtgc gtgttgtggt
     2521 aagccagcgg acgatcctca tcatctgatt ggtcatggtc agggcggaat gggaacaaaa
     2581 tcccacgata ttttcacgct accgctgtgt cgggagcatc acaacgagct tcatgcggat
     2641 ccgctggcgt tcgaagaaaa gcatggttcc caggttgatt taatttttcg ttttcttgat
     2701 cacgcctttg cgactggcgt gctcgggtaa aagaggtgac tgatgctcat agatttggtt
     2761 ttaccttacc cgccgacggt gaacacttac tggcgacgcc gtggcagcac atattttgta
     2821 tcaaaagccg gggagcgtta tcgccgggca gtggtgctta ttgttcgcca gcagcgactg
     2881 aaattaagcc tgtccggacg gctggcaata aaaattattg cagagccacc ggataagcgt
     2941 cgtcgtgacc tggacaatat tctgaaagca ccgctggatg cgctgacgca tgcgggagtg
     3001 ttaatggacg atgagcagtt tgatgaaatc aatattgtac gtggtcagcc agtatctggt
     3061 ggacggctgg gtgtgaagat ttacaaaatt gagagtgagt gagcgtaaat atgatatatc
     3121 cggaaattac aggcaaaagc ggcgaacatt tacgcctgaa cacgctggaa gcagtctgga
     3181 tccaggggaa attacggatg tgggggcggt ggtcgtatat cggtgggggt aaatccggaa
     3241 atatgtttaa ccggttactg gtttcgaaaa agctgacgaa aacagcagtt aatgaggttt
     3301 tacgcagcat gaagaaatcc gggctggaaa aaccggaact tgaggcattt tttcgggata
     3361 tgaccagagg gaagcagaag agctggttgt cacattgtac agacacagag gcgttgatta
     3421 ttgatcgcgt tatcagtgag gtgcttgggg aatatcccgg gctaatcaat attctccggc
     3481 aaaggtacga aggacgggga atgagtaaga gaaaaatggc agaatgttta aatcgtactc
     3541 acccggaatg gtgtttcagc acatgtgaga aacgtattgc aggttggtta gccgtggctg
     3601 aacacatgct ttatgtacct atgcacgatt catttcgata aaaaaagctt gcttttttac
     3661 gcagaaacag cttgaattcc tgtaagcttc gcaaagctgt atcgcgaggc gaaatgcaag
     3721 ttttttcgca caaggaagcc accggaaggt ggtttttttg tgtccgtaat atacagcagc
     3781 gcaataaatt cgctggtggt tattaatacc gttctttcag gttgctggct ttttcgacaa
     3841 gagttattgg tgtgtcacgt taaccggaaa agggaaaaag acatgctgaa acagcaggat
     3901 atgacagaaa ccgccagagt ggtgtttaat gaattaagcg ttaccgaccc ggcgacagtc
     3961 ggggagattg cgcagaatac ttacctttca cgcgaacgct gccagttaat actgacccag
     4021 ctggttatgg cgggtctggc agactatcag tttggttgtt acagacgcct tcagtcctga
     4081 aggctttttt atttgtggta aatgggcggc tggtgggtgt aaggggcacc caccagccat
     4141 ctgctcatgc gttgggttca caagcaaacc tcaggcccac tgctttgcgc aaaagcagaa
     4201 tgagcctatc agagacaggc ttaatgatcc atgcttaata ctgtaaaaat atccagttgt
     4261 gagttaatca acgccgactg cctggaattt atgcggtcgt tacccgaaaa ttctgttgac
     4321 ctgatagtca cggacccgcc gtacttcaaa gtgaaacccg agggctggga taaccagtgg
     4381 gcgggtgatg aagattacct gaagtggctg gaccagtgtc ttgcgcagtt ctggcgggtg
     4441 ctgaaacctg ccggaagtct ttacctgttc tgtggccatc gtctggcatc tgacaccgaa
     4501 atcatgatgc gtgagcggtt taacgtgctg aaccatatca tctgggcaaa gccgtccgga
     4561 cgctggaacg ggtgcaacaa ggaaagcctg cgggcgtatt tccccgccac agagcgcatt
     4621 ctgttcgcag agcattatca ggggccgtat cgtccgaaag atgccgggta tgaggcgaag
     4681 ggtaggacac tgaaacagca tgtgatggcc ccgctgattg cttactttcg tgatgcgcgc
     4741 gctgtcctgg ggataacggc aaaacagatt gcagatgcca caggaaagaa aaacatggtg
     4801 tcgcactggt tcagtgccgg tcagtggcag ctgccgaacg aaagcgatta tctgaaatta
     4861 caggcactgt ttgcccgggt ggcagaagag aagcatcagc ggggtgaact ggaaaagccc
     4921 caccaccagc tggtggatac gtatgcctct ctgaaccgac agtatgcgga gctgcagagt
     4981 gaatataagc atctgcggcg gtatttcggt gtgacggtgc aggtgccgta caccgatgtg
     5041 tggacgtata aaccggtgca gtactatcca gggaaacatc cgtgcgaaaa accggcagaa
     5101 atgttgcagc agataatcag cgcaagcagt cgtccgggag acctggttgc agatttcttc
     5161 atggggtcgg ggtcgacagt gaaagcagcg atggcgctgg gacgtcgtgc aactggcgtt
     5221 gaactggaga ctgaacgttt tgagcagacg gtgcgggaag tacaggattt aatcattcgt
     5281 aacggatgag attgcggagt taatcatgcg tcgttattat tcagcaatcg gccctttagc
     5341 tcagcggtga gagcgagcga ctcataatcg ccaggtcgct ggttcaaatc cagcaagggc
     5401 caccaaccgc cactagctca tcaggaaaga acgtcaccct gtgcgagatt cggagtcccc
     5461 ggtggcggtc cattatcggt attctgcgtt gttagctcag ccggacagag caattgcctt
     5521 ctaagcaatc ggtcactggt tcgaatccag tacaacgcac cacaccacac ttatctgccc
     5581 tgactctctt ttgcgggctt tttattacag gaaagacacc ggacagtgaa atgttaaatg
     5641 cctcacaatt caggcagttg actgttgcct gacatgctga gcgtttgtta aaaaaatcct
     5701 gcatgatgaa tccccctgag cggcggggca taatgacaga tgtttggttg cgtattgtat
     5761 aggcaagttg cggattctgt ctggtcattg cagaattcac cgggaggcac ccggcatcat
     5821 gctgtataca gagattaggc atatatccag gcttctcatc gcaggagcct ttttacatgc
     5881 aaaaaaaagc ccgagtgggt tcgggcaaca gcatgagata cttgcattgt catttttatc
     5941 gtgtggattt taaccagggt ttataaggct gcgcaactgc gcggcctttt tcgttttgcg
     6001 ggctgcggtt ctcctctttt gattctcctt gtggccggac cgtggcccgc aactgttgag
     6061 gaaaatcccg gaaaggggag gaataatgac atttaaacat tatgatgttg tcagggcggc
     6121 gtcgccgtca gaccttgcgg aaaagctgac acacaaactg aaagagggct ggcaaccgct
     6181 attctgaacg cagccgggcg cacctccgcc ttcatcagtg gtaaggcacc ggaaatcaaa
     6241 ccctcgcccg gcggcaacac gccatcgggt ccgtctgcag atacgtccgt tcgcacaatc
     6301 tccctgctgc cggcagccgg agaggctgct gcgcagggct ggagcattaa ggatggcgga
     6361 attcagttgt cagatggtgt atttaagatc accaggcaga gcaataaaac ctggtccctg
     6421 acgcatccgg tggatgacgc aattaccctg ctgacacagg gcggcagact gaactgtaag
     6481 ttccgcctgt caggcgcact gaccaacaat cagttcgggc tggggattta tctgtatacg
     6541 ga
//
LOCUS       DBFIFI010000227         6528 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.227, whole genome shotgun sequence.
ACCESSION   DBFIFI010000227 DBFIFI010000000
VERSION     DBFIFI010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6528
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.227"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(76..2358)
                     /locus_tag="N7B39_004200"
     CDS             complement(76..2358)
                     /locus_tag="N7B39_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HGU2424066.1"
                     /translation="MAVKASGRFVPPSAFAAGTGKAFTGAYAWNAPREAVGRERPLTR
                     DEMRQVQGVLSTINRLPYFLRSLFTSRYDHIRRNKSPVHGFYFLTSTFQRRLWPRIER
                     VNQRHEMNTDASLLFLAERDHYARLPGMNDKELKKFAARISSQLFMMYGELSDAWVDA
                     HGEKESLFTDEAQAHLYGHVAGAARAFNISPLYWKIYRKGQMTTRQAYSAIARLFNDE
                     WWTHQLKGQRMRWHEALLIAVGEVNKDRSPYASKHAIRDVRARRQANLEFLKSCDLEN
                     RETGERIDLISKVMGSISNPEIRRMELMNTIAGIERYAAAEGDVGMFITLTAPSKYHP
                     TRQVRKGESKTVQLNHGWNDEAFNPKDAQRYLCRIWSLMRTAFKDNDLQVYGLRVVEP
                     HHDGTPHWHMMLFCNPRQRNQIIEIMRRYALKEDGDERGAARNRFQAKHLNRGGAAGY
                     IAKYISKNIDGYALDGQLDNDTGKPLKDTAAAVTAWASTWRIPQFKTVGLPTMGAYRE
                     LRKLPRGVSIADEFDERVEAARAAADSGDFALYISAQGGANVPRDCQTVRVARSPSDD
                     VNEYEEEVERVVGIYAPHLGARHIHITRTTDWRIVPKVPVVEPLTLKSGIAAPRSPVN
                     NCGKLTGGDTSLPAPTPTEHAAAVLNLIDEGILSWTEPGIMKVLRDLLSNELKCSNRS
                     QQSFTPFNGRSYFPAPSARLTRQERKTIPKIKVLLAQSDIQASYWELEALARGAVLDF
                     GHKRFKFDNDIDFFDRQREW"
     gene            complement(2348..2623)
                     /locus_tag="N7B39_004201"
     CDS             complement(2348..2623)
                     /locus_tag="N7B39_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HGU2424067.1"
                     /translation="MSIRIEIGERYVVTSDSFQFILHEKKRAESGKNAGQEWLAVVGY
                     YPKLSQLVSGLMHHDILTGSAKSFADLNAQVEQLSKRCSEAFGSYGR"
     gene            complement(2620..2844)
                     /locus_tag="N7B39_004202"
     CDS             complement(2620..2844)
                     /locus_tag="N7B39_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HGU2424068.1"
                     /translation="MPDNVDFIQEQQAELLERQINAARVKHCGASALVCEECDAPIPA
                     ARRAAYPSATRCVSCQSVFEAKNKRYRRTA"
     gene            complement(2847..3146)
                     /locus_tag="N7B39_004203"
     CDS             complement(2847..3146)
                     /locus_tag="N7B39_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HGU2424069.1"
                     /translation="MSCSHSVVLLNNALKIAVMKNGDLSLIQLCLDKEKRDITESVIA
                     IYQNELNLLSDVVNLLVKRAVFHKQISSVDELTKLTTELASYCADVSRKLNDKRS"
     gene            complement(3146..3370)
                     /locus_tag="N7B39_004204"
     CDS             complement(3146..3370)
                     /locus_tag="N7B39_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2732 family protein"
                     /protein_id="HGU2424070.1"
                     /translation="MHTVSENKCGKYALLLQQARAEAQADAATRFSSHLDAMIRHITK
                     AELSRVEIVELLSQESEKFHNIGLSRGEVL"
     gene            complement(3434..3934)
                     /locus_tag="N7B39_004205"
     CDS             complement(3434..3934)
                     /locus_tag="N7B39_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein B"
                     /protein_id="HGU2424071.1"
                     /translation="MTVMTLNLVEKQPAAMRRIIGKHLAVPRWQDTCDYYNQMMERER
                     LTVCFHAQLKQRHATMRFEEMNDVERERLVCAIDELRGAFSKRRQVGASEYAYISFLT
                     VSQRRTLFMHAGLTEKEFNQPYWRINEESCYWRDALFRALRELFSLFEYAPTILTSVK
                     PEQYLH"
     gene            complement(3931..4101)
                     /locus_tag="N7B39_004206"
     CDS             complement(3931..4101)
                     /locus_tag="N7B39_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424072.1"
                     /translation="MNEPRCIAQLLRNESPRAIDFTITHGKGRKGIIIRTKKQSPLKK
                     ALTFLKSRRVWK"
     gene            complement(4112..4387)
                     /locus_tag="N7B39_004207"
     CDS             complement(4112..4387)
                     /locus_tag="N7B39_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory phage cox family protein"
                     /protein_id="HGU2424073.1"
                     /translation="MNRQLVSVTDAVPYQEFAKLIGKTPRAVRGMIEKGKLPVIEITD
                     PQSVSGRAGEYWVYLPAWNNGLKLAYESRPKEIRDGWLMWLGLGEPR"
     gene            4509..4808
                     /locus_tag="N7B39_004208"
     CDS             4509..4808
                     /locus_tag="N7B39_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU2424074.1"
                     /translation="MSIDVSEKLKLIRESERLNRKEFSELTGVAYSSLSSYESRSKNA
                     GVEAIMKVLQHPRFTKYTLWFMTDQVAPEAGQIAPALAHFGQNETTSPHSGQKTG"
     gene            4924..5937
                     /locus_tag="N7B39_004209"
     CDS             4924..5937
                     /locus_tag="N7B39_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HGU2424075.1"
                     /translation="MAIKKLDDGRYEVDIRPTGRNGKRIRRKFDKKSEAVAFEKYTLY
                     NHHNKEWLSKPTDKRRLSELTQIWWDLKGKHEEHGKSNLGKIEIFTKITNDPCAFQIT
                     KSLISQYCATRRSQGIKPSSINRDLTCISGMFTALIEAELFFGEHPIRGTKRLKEEKP
                     ETGYLTQEEIALLLAALDGDNKKIAILCLSTGARWGEAARLKAENIIHNRVTFVKTKT
                     NKPRTVPISEAVAKMIADNKRGFLFPDADYPRFRRTMKAIKPDLPMGQATHALRHSFA
                     THFMINGGSIITLQRILGHTRIEQTMVYAHFAPEYLQDAISLNPLRGGTEAESVHTVS
                     TVE"
     gene            complement(6202..6519)
                     /gene="yegR"
                     /locus_tag="N7B39_004210"
     CDS             complement(6202..6519)
                     /gene="yegR"
                     /locus_tag="N7B39_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HGU2424076.1"
                     /translation="MKKIAAISLISVFLMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
BASE COUNT         1775 a         1645 c         1423 g         1685 t
ORIGIN      
        1 tgtataaaaa ttaaactata acaaacaatt cctcaacaat aacatattgt taccccgttt
       61 gaccgttttt ttcaactacc actcacgctg tctgtcaaaa aagtcgatat cgttatcaaa
      121 tttaaaacgc ttatgcccaa aatccaaaac agctccgcga gcgagagctt caagctccca
      181 ataactagcc tgaatgtcac tttgtgcaag aagaacctta attttcggga tagtctttct
      241 ctcctgcctt gtcaaccggg cggatggggc aggaaagtag cttcgaccat tgaaaggggt
      301 aaagctctgc tgtgaacgat tactgcattt cagttcatta ctcaataagt ctctaagtac
      361 cttcataatg cctggctcgg tccaacttag aatcccttca tctattaaat ttaacaccgc
      421 tgcagcatgc tcagtcggtg tgggagccgg taacgaagta tcaccaccgg tgagttttcc
      481 acagttattg acaggactcc gaggcgcggc gatgccgctt tttaaagtca aaggctcaac
      541 gaccggaact ttcggcacaa tgcgccagtc cgtcgttctg gtgatatgaa tatgacgcgc
      601 gccgagatgc ggcgcgtaaa tgccgaccac tctctcgact tcttcctcgt actcgttaac
      661 gtcatccgac gggctacggg cgaccctgac agtctgacaa tcgcgcggga catttgcccc
      721 accctgcgcg ctgatataca acgcaaaatc accactgtct gcggcagcgc gagcagcctc
      781 gacgcgttcg tcaaactcat cagcaatact gacgccgcga ggcaatttgc gtagttcacg
      841 gtaagccccc attgtcggca gtccaaccgt tttaaattgc ggaatgcgcc acgttgacgc
      901 ccatgcggta acagccgcgg cagtatcttt aagcggctta ccggtatcgt tatcgagctg
      961 accatccagt gcatagccgt cgatgttttt tgaaatgtat ttcgcgatat atcccgcagc
     1021 accgccccgg ttaaggtgtt ttgcctgaaa acggtttcgc gcagctcctc tttcgtctcc
     1081 atcctctttg agcgcgtagc gacgcatgat ttcgataatc tggttacgct ggcgtggatt
     1141 acaaaaaagc atcatatgcc agtgcggcgt tccgtcgtgg tgtggctcga cgacacgcaa
     1201 accgtagacc tgtaaatcat tatccttgaa tgccgtgcgc atcaggctcc agatgcggca
     1261 gagataacgc tgcgcatcct ttggattaaa tgcctcatcg ttccagccgt gattaagctg
     1321 aacggtttta ctttcgcctt ttctgacctg acgtgtcggg tgatactttg acggcgcggt
     1381 cagcgtgata aacatcccca catcaccctc tgcggcggcg taacgctcaa taccggcaat
     1441 ggtgttcatc agctccatcc ggcgaatttc aggattagaa atactgccca tcaccttact
     1501 gataaggtcg atgcgctcgc cggtttccct gttttcaagg tcacacgatt taagaaattc
     1561 cagatttgcc tggcggcgcg cacgcacatc acgaatggcg tgtttactgg cataaggaga
     1621 acggtcttta ttgacctccc cgacagctat cagtaacgct tcatgccagc gcatacgctg
     1681 gcctttaagc tgatgagtcc accactcatc gttaaacaga cgggcaatgg cagaatatgc
     1741 ctgcctcgtg gtcatctgcc ctttacggta tattttccag tagagagggg aaatattgaa
     1801 agcacgtgca gcgccagcaa catgaccata gaggtgagcc tgcgcctcat ccgtaaacag
     1861 cgattctttt tcgccatgcg catccaccca ggcatcactg agttccccat acatcatgaa
     1921 aagctgcgat gagatacggg cagcaaactt tttcagctcc ttgtcattca ttcccggcag
     1981 gcgcgcatag tggtcacgct ctgccagaaa cagtaacgac gcgtcggtgt tcatttcatg
     2041 gcgctgattc acacgctcaa tgcgcggcca taaacgacgc tgaaaagtgg atgtgaggaa
     2101 ataaaacccg tgcaccgggc ttttattgcg ccggatgtgg tcatagcgtg aagtaaacag
     2161 cgagcgcaaa aagtaaggca ggcggttaat cgtggataaa acaccttgca cctgacgcat
     2221 ctcgtcacgt gtaaggggtc tttcgcgccc gacggcctcg cgtggcgcgt tccatgcata
     2281 agcaccggta aacgccttac cggtgcctgc agcaaatgct gacggaggga caaaacgccc
     2341 ggaggcttta acggccatat gagccaaaag cctctgagca acgcttgctg agttgctcaa
     2401 cctgcgcgtt taaatcagca aaagactttg cgcttccggt cagaatatcg tgatgcatca
     2461 ggccggaaac gagctggctt aatttcgggt aataaccaac caccgccagc cattcctgac
     2521 cggcgttttt accgctttcc gctctctttt tctcgtggag aataaactga aagctgtcac
     2581 tggtaacgac ataacgttcg ccaatttcaa tacgaatact catgccgttc tccggtaacg
     2641 tttgtttttt gcttcaaaga ctgactgaca ggaaacacaa cgcgtggctg acgggtaagc
     2701 cgcacgacgg gcagcaggta ttggcgcgtc acactcttcg caaaccagcg cagaagcacc
     2761 gcaatgtttt acccttgccg cgttaatctg gcgctccagt aattcagcct gttgttcctg
     2821 aataaaatct acgttgtccg gcattatcag ctccttttat cgttaagttt cctggataca
     2881 tcagcgcaat aactggcaag ttctgtcgtt aattttgtca gttcatccac tgaggaaatt
     2941 tgcttgtgga atacagcgcg tttaacaagt aaattgacca catcagacag gaggtttaat
     3001 tcattctgat aaatcgcgat aacagattca gttatgtcgc gtttttcttt atcaaggcaa
     3061 agttgaataa gagacaaatc gccatttttc ataacggcga tttttaaggc gttattcagt
     3121 aatacaactg aatgagaaca ggacatcaaa gcacctcccc gcgagacaat ccgatattgt
     3181 gaaatttttc cgactcctga ctgagcagct cgactatctc cacgcgggat aactccgcct
     3241 ttgtgatatg gcgaatcatg gcgtcaagat gagaagaaaa gcgtgtcgct gcgtcggcct
     3301 gtgcttcggc tctggcctgt tgcagcagta atgcgtattt accgcacttg ttttcagaaa
     3361 ctgtatgcat gactttctcc aggcaaaaag aagccccgca cgattaagtg cgttaaaaac
     3421 tctggttaat tatttaatgc agatattgct ctggttttac cgacgtcaga attgtcggtg
     3481 catactcaaa caggctgaat aattcacgta atgcacggaa taaagcatca cgccagtaac
     3541 atgattcttc attaattcgc cagtatggct ggttgaattc tttttcagtc aatccggcat
     3601 gcataaataa agtacgacgc tgactgactg ttaaaaaact aatatatgca tactcacttg
     3661 cgccaacctg acggcgtttt gagaatgccc cacgcaattc atcaattgca caaaccagcc
     3721 gttcacgttc gacgtcgttc atttcttcaa aacgcatcgt tgcgtgacgc tgttttaact
     3781 gcgcatggaa gcaaaccgtt agccgttcgc gttccatcat ctgattataa taatcacatg
     3841 tatcctgcca gcgagggacg gcaagatgct tgccaattat ccggcgcata gctgctggct
     3901 gtttttcaac gagattgagc gtcatcactg tcatttccag accctccggc ttttcagaaa
     3961 ggtcagagcc ttttttaacg gactctgttt tttggtgcgg ataatgattc ccttacgccc
     4021 cttaccgtgg gtgatggtga agtcaatcgc cctggggctt tcgttacgca ataactgagc
     4081 aatacaacga ggctcattca tacggttctc cttaacgtgg ttcaccgaga cctaaccaca
     4141 tcaaccagcc gtcacgaatc tctttaggac ggctttcata agccagtttt agtccgttat
     4201 tccatgccgg aaggtatacc caatattcac cagcacgccc cgatactgac tgagggtcag
     4261 taatctcaat aactggtaat ttccctttct caatcatgcc ccttacagct cttggagttt
     4321 taccaatgag ttttgcaaac tcctgataag gcacggcatc agtcacgctt acaagctgtc
     4381 tattcatctg ctacgattct cccttagtgc ttctaatggc tcctaatggc taattattgc
     4441 ctaaaaggat aactccagaa gcacaacatt tcacactatc agcaagaaat tacgcaatcg
     4501 gagtaattat gtcaatagac gtttcggaga agttgaagct aatccgtgaa tctgaaaggt
     4561 taaaccgtaa agaattcagt gaattaactg gtgtagccta cagctcactt tcgagctatg
     4621 agagccggtc aaaaaacgct ggagttgaag ccataatgaa ggtcttacaa catcctagat
     4681 ttactaaata tactttgtgg ttcatgactg atcaggtagc tccagaagcc gggcaaattg
     4741 cgcccgctct cgcacacttt gggcaaaacg aaacaacgtc gccccactcc ggtcaaaaga
     4801 ctggttaaca atttatcgtg aatatattca tcacaagtgc ctactattgg tggctaaatt
     4861 tcagccacca cgaaaaaagc gattagtagt agcaaaaaaa agtaccactc ggagggtttt
     4921 ctgatggcaa tcaaaaaact cgatgatggt cgatatgaag tggacatccg ccctactgga
     4981 cgtaacggaa aacgcatccg taggaagttt gataagaaaa gcgaagctgt cgctttcgaa
     5041 aaatacacgt tgtacaacca ccacaataaa gaatggctat caaaaccaac agacaaacga
     5101 cgtctgtcgg aactgacaca gatctggtgg gatttaaagg gtaaacacga agagcatggg
     5161 aaatctaatc ttggaaaaat tgaaatcttc acaaaaataa cgaatgaccc atgcgcattt
     5221 caaatcacga aatcccttat cagccagtac tgcgccaccc gaagaagtca gggtattaaa
     5281 ccttcgagta tcaatcgtga tttaacatgt attagcggca tgtttacagc cctgattgaa
     5341 gcggagttat tctttggtga gcatcctatc agagggacaa agaggcttaa ggaggaaaaa
     5401 ccagaaacag gctatctcac acaggaagaa atagccttac tgcttgcagc acttgacggc
     5461 gacaataaaa agattgcgat tctttgccta agtacaggag cacgttgggg agaagcagct
     5521 cgtttgaaag cagaaaatat catccataac cgcgtcacgt ttgttaaaac gaaaacaaac
     5581 aaaccacgca ccgtcccgat ctcagaggct gttgccaaaa tgatcgcgga taacaaacga
     5641 ggttttttat tccctgatgc tgattaccct cgcttcagac gaacaatgaa agcaataaaa
     5701 ccggatttgc caatggggca agccacacat gcactaaggc acagctttgc cactcatttc
     5761 atgattaatg gaggaagtat tatcacgcta caacggatac taggtcacac gcggattgag
     5821 caaactatgg tttacgctca ttttgcgcca gagtaccttc aggacgccat ttctcttaat
     5881 ccgctaagag gtggtactga ggccgagagt gtccacacag tgtccacagt agagtaacgt
     5941 ttaagggctt tcagtggtaa tttatgccgc tcaaacccgc attgtaccgt tgaaagcccc
     6001 tactggtgac accctaaatc tcccttacac gggcttattt tttatgcata agccctatcc
     6061 ctgataaccg tcttccattg accacatcga tagaatctcc cttcatagca cgatgccttt
     6121 cacgtaacgg catcgtgctc gcacaggttc cggctaagca caaccagaac gcgcatgttt
     6181 gacgcttacc aaaaaatatt ctcactctcc acatttgaat gtcagacgag cgacgccatg
     6241 taatcctgca ccttctgtct tcaggtcaac tatctgcatt tttttgccct gagtaacaca
     6301 gaaatgggct gcatcatttt ttactatatt ttctgcacca gatattctgc ccctggctaa
     6361 agaagcttcg gcttcggtgt agtattggtt atcgagttta cgctgaatat tacttttata
     6421 tgcaagacca aatttaccga tacttgtctc atcattatgc acagcacaac cagacataag
     6481 aaaaacacta attaatgata tagcagctat ctttttcatc tcaccttc
//
LOCUS       DBFIFI010000228         6499 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.228, whole genome shotgun sequence.
ACCESSION   DBFIFI010000228 DBFIFI010000000
VERSION     DBFIFI010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6499
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.228"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..754
                     /locus_tag="N7B39_004211"
     CDS             <1..754
                     /locus_tag="N7B39_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU2424077.1"
                     /translation="IRNVLLPCFANAFTEAGFATITFDYRGFGESDGERGRLVPAMQT
                     EDIISVINWAEKQECIDNQRIGLWGTSLGGGHVFSAAAQDQRVKCIVSQLAFADGDVL
                     VTGEMNESERASFLSTLNKMAEKKKNTGKEMFVGVTRVLSDDESKVFFEKIKARHPEM
                     DIKIPFLTVMETLQYKPAESAASVQCPVLVVIAGQDSGNPPEQGRALYDAVASGTKEL
                     YEEADACHYDIYEGAFFERVVAVQTQWFKQYL"
     gene            857..1417
                     /locus_tag="N7B39_004212"
     CDS             857..1417
                     /locus_tag="N7B39_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HGU2424078.1"
                     /translation="MAEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTQDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            1550..1762
                     /locus_tag="N7B39_004213"
     CDS             1550..1762
                     /locus_tag="N7B39_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HGU2424079.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGSRAVILEVSGRWMEAAEAWRRAAG
                     VAPRTDWQQFARKRAEHCHRRCRGRG"
     gene            2007..2468
                     /locus_tag="N7B39_004214"
     CDS             2007..2468
                     /locus_tag="N7B39_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HGU2424080.1"
                     /translation="MRKYIPLVLFIFSWPVLCADIHGRVVRVLDGDTIEVMDSRKVVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRMLGQVYAPDGMNVN
                     QFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            2514..2723
                     /gene="hha"
                     /locus_tag="N7B39_004215"
     CDS             2514..2723
                     /gene="hha"
                     /locus_tag="N7B39_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HGU2424081.1"
                     /translation="MEKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSTSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            2761..3099
                     /locus_tag="N7B39_004216"
     CDS             2761..3099
                     /locus_tag="N7B39_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424082.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSGGEYDASHLRPERRRRDILLEEVLRQAGIPLLRSHDARKLLQMTGEWLNTTG
                     AAQQSPEHRS"
     gene            3339..3593
                     /locus_tag="N7B39_004217"
     CDS             3339..3593
                     /locus_tag="N7B39_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HGU2424083.1"
                     /translation="MSQTENAVTSSSGAKRAYRKGNPLSDAEKQRLSVARKRASFKEV
                     KVFLEPKYKAMLMQMCHEDGLTQAEVLTALIKSEAQKRCV"
     gene            3829..3903
                     /gene="tap"
                     /locus_tag="N7B39_004218"
     CDS             3829..3903
                     /gene="tap"
                     /locus_tag="N7B39_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HGU2424084.1"
                     /translation="MLGKVQDFFLCSLLLRIVSAGWCD"
     gene            3896..4753
                     /gene="repA"
                     /locus_tag="N7B39_004219"
     CDS             3896..4753
                     /gene="repA"
                     /locus_tag="N7B39_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HGU2424085.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSKGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTPALFAALDV
                     SEDAVAAARRSRVEWENRLRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDAGRERQDIVTLVKRQLTREISEGRFTANREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            5666..5935
                     /gene="yacA"
                     /locus_tag="N7B39_004220"
     CDS             5666..5935
                     /gene="yacA"
                     /locus_tag="N7B39_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015056448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin YacA"
                     /protein_id="HGU2424086.1"
                     /translation="MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNTWFRDQVEAGRQQLGRGDVLPHDMVESSAAAWRDEMSRKVAGK"
     gene            5935..6213
                     /gene="yacB"
                     /locus_tag="N7B39_004221"
     CDS             5935..6213
                     /gene="yacB"
                     /locus_tag="N7B39_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin YacB"
                     /protein_id="HGU2424087.1"
                     /translation="MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAG
                     QPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVYILRVLHTAQKWP"
BASE COUNT         1772 a         1462 c         1732 g         1533 t
ORIGIN      
        1 tatcaggaat gttctgctcc cttgctttgc gaatgccttc acagaagcag gattcgcaac
       61 gattactttt gattatcgag gattcggtga gagtgatgga gaacgagggc gtctggttcc
      121 tgcaatgcag accgaagata ttatttcagt tatcaattgg gcagaaaagc aggaatgtat
      181 tgataatcag cgtattgggt tatggggaac atcgctggga ggggggcatg tcttcagtgc
      241 agctgcgcag gaccaacgcg taaaatgtat tgtcagccag ctggcttttg ctgacggtga
      301 tgttctggtt accggagaga tgaatgaatc ggagagagcg tcttttctat ccacgctgaa
      361 taaaatggca gagaagaaga aaaacaccgg gaaagagatg tttgtggggg ttacacgggt
      421 actaagtgat gatgaatcga aggtattctt cgagaaaatc aaagcccggc atcccgaaat
      481 ggatataaaa atacctttcc ttacggtcat ggaaacgttg caatataaac cggcagagag
      541 tgcagccagc gtccagtgcc cagttttggt tgttattgcc ggccaggata gcggtaatcc
      601 accggaacag ggaagagcat tatatgatgc tgttgcttct ggtactaagg aattatacga
      661 ggaagctgat gcatgccatt atgatattta tgagggcgcg ttttttgaac gtgtagtagc
      721 tgtgcagaca caatggttta aacaatatct ttaattgtca gtatttatac gttaaaggtc
      781 gttgaaatta acttcctgtt acgatgagcc gtttcaacgg cctgcagggc atattttcac
      841 gaaggtcaga gtaagcatgg cagagcaaaa acgaccggta ctgacactga agcggaaaac
      901 ggaaggagag acgcctgtcc gcagccggaa aaccatcatc aatgtcacca cgccaccaaa
      961 atggaaggtg aaaaagcaga agctggccga gaaagcagcc cgggaagcgg agctggcggc
     1021 aaaaaaagcg caggccagac aggcgctgtc catttatctg aacctgccca cgcaggatga
     1081 ggccgtgaac accctgaaac cctggtggcc gggattattt gacggtgaca cgccccggct
     1141 tctggcctgc ggtatccggg acgtgttact ggaagacgtg gcgcagcgga atatcccgct
     1201 ctcgcataaa aagctgcgca gggcgctgaa ggccatcacc cgttcagaaa gctatctgtg
     1261 tgccatgaaa gccggtgcct gccggtatga cacggaaggg tatgtgacgg agcatatttc
     1321 tcaggaggag gaagcgtatg cggcagcgcg tctggataaa atccgccgcc agaaccggat
     1381 aaaggcagaa cttcaggccg tgcttgatga gaaataaaaa aagcctccct cagagagagg
     1441 aggcagggaa ataaggctta aaaggaaata acttccgtac ccgaaaggaa gccaattcag
     1501 tagattattt aacaccataa tactgacggg ttaaagagga gtgataaaga tgaatggatt
     1561 cagaaacagt tcacggaacg gtcaggtctg gcgttaccag cgtgccggaa gccgggccgt
     1621 tattctggaa gtcagtggac gctggatgga agcggcagaa gcatggcgac gggctgccgg
     1681 tgttgcaccc cggactgact ggcagcagtt tgcccgaaaa agggctgaac actgccaccg
     1741 gcgctgccga ggaagggggt aacgaaaaaa gcagccttca ttactccagg gattcagaag
     1801 gctgaataat gcatgagatt tgcttttttt aatatccggt cgggcagtat gaccggccag
     1861 ttctgagtag cacagagttt tttccgtatt aagtcgtgat tttatatttt gtgattaatt
     1921 tcacaaaata aggtgttatt aagtgtgtgc tgcaatattc aggatgcctg aaatacaggt
     1981 gctatttatc tgatacggag aataacatga ggaaatatat tccactggta ttatttatct
     2041 tttcatggcc ggtattgtgt gcagatattc atggacgggt tgttcgtgta ctggacggtg
     2101 ataccatcga ggtcatggac tcacggaagg tggtgcggat caggctggta aatattgatg
     2161 cgccggaaaa gaaacaggat tatggccggt ggtcgacaga catgatgaaa tcactggttg
     2221 caggaaaaac ggttaccgtc acatattttc agcgagatcg ttatggtcgt atgctcgggc
     2281 aggtttatgc accagacgga atgaatgtta atcagtttat ggtccgggcc ggggccgcct
     2341 gggtatatga gcagtacaac acagatcccg tgctgcctgt ccttcagaac gaagccagac
     2401 aacaaaaacg tgggctctgg tcagatgctg atccggttcc cccctggata tggagacacc
     2461 gtaaataaag cgatggcaat cttatcattc atctgttctc agcgggaata aaaatggaaa
     2521 aaacaaaaca ggaatggctg tatcaattac ggcgatgttc atcagtcaat acgctggaaa
     2581 aaattattca taaaaacaga gacagtttat cgaccagtga acgtgaatca tttaattcag
     2641 cagcagacca ccggttagca gaattaatca ccggaaagtt atatgacagg ataccaaaag
     2701 aaatatggaa atatgtaagg taatagttca gcataattaa ttaccaggag cagataaaca
     2761 atgaagctga ttatttttat tctcattgtt ctgattattg cagcgttact tatccggatt
     2821 atcctcagaa gtgtaaatca gcattctcct ttgctgatgc aattgcatgc ggcaggtata
     2881 cggaccggtg atgcagagcg aatactgtcc ggcggtgaat atgatgccag ccatttacga
     2941 cctgaacgca gacgccggga tattcttctg gaagaggttc tgaggcaggc tggtattccg
     3001 ttgctcagaa gccacgatgc cagaaaactg ctgcagatga ccggagaatg gctgaataca
     3061 acaggggctg ctcagcagtc accggaacat cgtagctgac gccttcgcgt tgctcagttg
     3121 tccaaccccg gaaacgggaa aaagcaagtt ttccccgctc ccggcgtttc cataactgaa
     3181 aaccatacta tttcacagtt taaatcacat taaacgacag taatccccgt tgatttgtgc
     3241 gccaacacag atcttcgtca caattctcaa gtcgctgatt tcaaaaaact gtagtatcct
     3301 ctgcgaaacg atccctgttt aagtattgag gaggcgagat gtcgcagaca gaaaatgcag
     3361 tgacttcctc atctggcgca aaacgagcat acagaaaggg gaatccgctt tctgatgcag
     3421 agaaacaaag attatcagtg gcccgtaaaa gagcttcgtt caaggaagta aaagtatttc
     3481 ttgaaccaaa gtataaggcc atgctcatgc aaatgtgtca tgaagatggc ctgactcagg
     3541 ctgaagttct gaccgcactg ataaaaagtg aagcgcaaaa acgatgtgtg tgatgatggg
     3601 cttacattct tgagtgttca gaagattagt gctagattac tgatcgttta aggaattttg
     3661 tggctggcca cgccgtaagg tggcagggaa ctggttctga tgtggattta caggagccag
     3721 aaaagcaaaa accccgataa tcttcagaaa gtttggcgac tgagaagatt accggggccc
     3781 acttaaaccg tatagccaac aattcagcta tgcggggagt atagttatat gctcggaaaa
     3841 gttcaagact tctttctgtg ctcgctcctt ctgcgcattg taagtgcagg atggtgtgac
     3901 tgatcttcaa caaacgtatt accgccaggt aaagaacccg aatccggtgt tcacaccccg
     3961 tgaaggtgcc ggaacgctga agttctgcga aaaactgatg gaaaaggcgg tgggcttcac
     4021 ctcccgtttt gatttcgcca ttcatgtggc acatgcccgt tcgaagggac tgcgtcggcg
     4081 catgccaccg gtactgcgtc gccgggctat tgatgcgctg ctgcaggggc tgtgttttca
     4141 ctatgacccg ctggccaacc gtgtccagtg ctccatcacc acactggcca ttgagtgcgg
     4201 actggcgacg gagtctgctg ccggaaaact ctccatcacc cgtgccaccc gggccctgac
     4261 gttcctgtca gagctgggac tgattaccta ccagacggaa tatgacccgc ttatcgggtg
     4321 ctacattccg accgacatca cgttcacacc ggctctgttt gctgcccttg atgtgtctga
     4381 ggatgcagtg gcagctgcgc gccgcagccg tgttgaatgg gaaaacaggc tgcgcaaaaa
     4441 gcaggggctg gataccctgg gtatggatga actgatagcg aaagcctggc gttttgtgcg
     4501 tgagcgtttc cgcagttacc agacagagct taagtcacgg ggaataaagc gtgcccgtgc
     4561 gcgtcgtgat gcgggcaggg aacgtcagga tatcgtcacc ctggtgaaac ggcagctgac
     4621 gcgtgaaatc tcggaagggc gcttcactgc taatcgtgag gcggtaaaac gcgaagtgga
     4681 gcgtcgtgtg aaggagcgca tgattctgtc acgtaaccgc aattacagcc ggctggccac
     4741 agcttccccc tgaaagtgac ctcctcagaa taatccggca cgcgccggag gcattcgcac
     4801 gcctgaagcc cgccggtgca caaaaaatca gcaccacatc ccaaaaacaa cctcatcatc
     4861 taccttctgg tgcatccggt cccccctgtt ttgatacaaa acacgtctca cagacgggca
     4921 attttgttta tccacattaa actgcaaggg atttctctgt aagattacaa ccgttcatgt
     4981 cataaagcac cagccgccag gcttacaggg tgcaatgtat cttttaaaca cccgtttata
     5041 tctcctttaa actacttaat tacattcatt taaaaaaaag acttattcac tgcctgtcct
     5101 gtggacagac agatatgcac ctcccaccgc aagcggcggg ccccaagcgg agctacttta
     5161 gttacaacac acaaacagaa ccaccaggaa aaaccccagt cccgcgcaga actgaaacca
     5221 caaagcccct cctcataact gaaaagcggc cccgccccgg cccgaagggc cggaacagag
     5281 tcgcttttaa ttatgaatgt tgtaactaca ttatcatcgc tgtcagtctt ctcgctggaa
     5341 gttctcagta cacgctcgta agcggccctc acggcccgct aacgcggaga tacgccccga
     5401 cttcgggtaa accctcatcg ggaccactcc gaccgcgcac aaaaactctc tcatggctga
     5461 aagcgggtat ggtctggcag ggctggggat gggcaaggtg aaatctgtca atcagtaccg
     5521 gcttacgccg ggcttcggcg gttttactcc ggtatcacat gtaaccacag cgtgccgcct
     5581 tccatgccgc tgacgcggca tatcctggtg acgatgtctg aatcgttata tactgtgtat
     5641 atacgtagta atgacgaggt gataaatggc acaggttaat atgagtttaa gaatcgacgc
     5701 tgaactgaag gatgctttta tggccgctgc aaaaagcatg gaccgtaatg gctctcagtt
     5761 aatccgggat tttatgcgcc agaccgttga acggcagcat aatacctggt ttcgtgacca
     5821 ggttgaggca ggacgtcagc aactcgggcg cggcgatgtg cttccccatg acatggtcga
     5881 atcttccgct gctgcatggc gggatgaaat gagcaggaag gttgccggta aatgatggag
     5941 atattctgga ccatgctggc cagccaggac aggaagcgca ttcgtgagta cattgcggag
     6001 cagaacctga tggctgccat tgaactggac gaacggattg gttattcagc cagcagcctg
     6061 gccgggcagc cgtataaagg ccgcaacggt cgtgtggaag gtacccggga actggttatt
     6121 cacccccatt ttgttctggt ttatgaggtc gacagccagt ggggaaaagt gtatatcctg
     6181 cgcgtattgc atactgcaca gaagtggcca tagaggctgt atggaatcag ttgtaaatca
     6241 tgacgaagaa actagcccag gatgatgtgg tattgatgga atggtctatg acacaggctc
     6301 cggtcattct gttaacgcat tcgcgtatgg aatgaactgt tatttcagtt tgaatcccgg
     6361 gaggtcagat ggatttttgc tgtgaatacg aaatccgtta cgaacctaac tggtcagtgc
     6421 tatctatgta ttgtgtctgt ggcatatgag ggaaaataga tagtgttagt aagatattga
     6481 gattttttag taattattt
//
LOCUS       DBFIFI010000229         6451 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.229, whole genome shotgun sequence.
ACCESSION   DBFIFI010000229 DBFIFI010000000
VERSION     DBFIFI010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6451
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.229"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            393..1652
                     /gene="codB"
                     /locus_tag="N7B39_004222"
     CDS             393..1652
                     /gene="codB"
                     /locus_tag="N7B39_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015209 - cytosine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HGU2424088.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSLIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEANSVE"
     gene            1642..2925
                     /gene="codA"
                     /locus_tag="N7B39_004223"
     CDS             1642..2925
                     /gene="codA"
                     /locus_tag="N7B39_004223"
                     /EC_number="3.5.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HGU2424089.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLQDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKLEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYSIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(3058..3957)
                     /gene="cynR"
                     /locus_tag="N7B39_004224"
     CDS             complement(3058..3957)
                     /gene="cynR"
                     /locus_tag="N7B39_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HGU2424090.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIHLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHLPELEAIPL
                     LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAIPLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALEECADVGENESR"
     gene            4067..4726
                     /gene="cynT"
                     /locus_tag="N7B39_004225"
     CDS             4067..4726
                     /gene="cynT"
                     /locus_tag="N7B39_004225"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HGU2424091.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            4757..5227
                     /gene="cynS"
                     /locus_tag="N7B39_004226"
     CDS             4757..5227
                     /gene="cynS"
                     /locus_tag="N7B39_004226"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009439 - cyanate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HGU2424092.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARQVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            5233..6414
                     /gene="cynX"
                     /locus_tag="N7B39_004227"
     CDS             5233..6414
                     /gene="cynX"
                     /locus_tag="N7B39_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HGU2424093.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSALLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAALTPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQSTREVASSHKTTTIPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LALQLVGFCGFIWLPLQLPVLWAMVCGLGLGGAFPLCLLLALDHSVQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSIRGNYLMDWAFHALCVVGLMIITLRFAPARFPQLWVKE
                     A"
BASE COUNT         1326 a         1714 c         1808 g         1603 t
ORIGIN      
        1 cgacaccgtg ctcagtgcct gatgcgacgc tgatgcgtct tatcatgcct acaggcctga
       61 accgtaggtc ggataaggcg ctcgcgccgc atccgacacc gtgcgcagat gcctgatacg
      121 acgcaggcgc gtcttatcat gcctacaggc ctgaaccgta ggtcggataa ggcgctcgcg
      181 ccgcatccga caccgtgcgt agttgcctga tgcgacgctg acgcgtctta tcatgcctac
      241 ccactgtttt tacaccgata attttccccc cacctttttg cactcattca tataaaaaat
      301 atatttcccc acgaaaacga ttgcttttta tcttcagatg aatagaatgc ggcggatttt
      361 ttgggtttca aacagcaaaa agggggaatt tcgtgtcgca agataacaac tttagccagg
      421 ggccggtccc gcagtcggcg cggaaagggg tattggcatt gacgttcgtc atgctgggat
      481 taaccttctt ttccgccagt atgtggaccg gcggcactct cggaaccggt cttagctatc
      541 atgatttctt cctcgcagtt ctcatcggta atcttctcct cggtatttac acttcatttc
      601 tcggttacat tggcgcaaaa accggcctga ccactcatct tcttgctcgc ttctcgtttg
      661 gtgttaaagg ctcatggctg ccttcactgc tgctgggcgg cacccaggtt ggctggtttg
      721 gcgtcggtgt ggcgatgttt gctattccgg tgggcaaggc aaccgggctg gatattaatt
      781 tgctgattgc cgtttccggt ttactgatga ccgtcaccgt cttctttggc atttcggcgc
      841 tgacggttct ttcgttgatt gcggttccgg ctatcgcctg tcttggcggt tattccgtgt
      901 ggctggccgt taacggcatg ggcggcctgg acgccttaaa agcggtcgtt cccgcacaac
      961 cgttagattt caatgtcgcg ctggcgctgg ttgtggggtc atttatcagt gcgggcacgc
     1021 tcaccgctga ctttgtccgc tttggtcgca atgccaaact ggcggtgctg gtggcgatgg
     1081 tggccttttt cctcggcaac tcgttgatgt ttattttcgg tgcagcgggc gctgcggcac
     1141 tggggatggc ggatatctct gatgtgatga ttgctcaggg cctgctgttg cctgcgattg
     1201 tggtgctggg gctaaatatc tggaccacca acgataacgc gctctatgcg tcgggtttag
     1261 gtttcgccaa cattaccggg atgtcgagca aaaccctttc ggtaatcaac ggtattatcg
     1321 gtacggtctg tgcattatgg ctgtataaca attttgtcgg ctggttgacc ttcctttcgg
     1381 cagctattcc tccagtgggt ggcgtgatca ttgccgacta tctgatgaac cgtcgccgct
     1441 atgagcactt tgcgaccacg cgtatgatga gtgtcaattg ggtggcgatt ctggcggtcg
     1501 ccctggggat tgctgcaggc cactggttac cgggaattgt tccggtcaac gcggtattag
     1561 gtggcgcgct gagctatctg atccttaacc cgattttgaa tcgtaaaacg acagcagcaa
     1621 tgacgcatgt ggaggctaac agtgtcgaat aacgctttac aaacaattat taacgcccgg
     1681 ttgccaggca aagaggggct gtggcagatt catctgcagg acggaaaaat cagcgccatt
     1741 gatgcgcaat ccggcgtgat gcccataact gaaaacagcc tggatgccga acaaggttta
     1801 gttataccgc cgtttgtgga gccacatatt cacctggaca ccacgcaaac cgccggacaa
     1861 ccgaactgga atcagtccgg cacgctgttt gaaggcattg aacgctgggc cgagcgcaaa
     1921 gcgttattaa cccatgacga tgtgaaacaa cgcgcatggc aaacgctgaa atggcagatt
     1981 gccaacggca ttcagcatgt gcgtacccat gtcgatgttt cggatgcaac gctaactgcg
     2041 ctgaaagcaa tgctggaagt gaagctggaa gtcgcgccgt ggattgatct gcaaatcgtc
     2101 gccttccctc aggaagggat tttgtcgtat cccaacggtg aagcgttgct ggaagaggcg
     2161 ttacgcttag gggcagatgt agtgggggcg attccgcatt ttgaatttac ccgtgaatac
     2221 ggcgtggagt cgctgcataa aaccttcgcc ctggcgcaaa aatacgaccg tctcatcgac
     2281 gttcactgtg atgagatcga tgacgagcag tcgcgctttg tcgaaaccgt tgctgccctg
     2341 gcgcaccgtg aaggcatggg cgcgcgagtc accgccagcc acaccacggc aatgcactct
     2401 tataacgggg cgtatacctc acgtctgttc cgcttgctga aaatgtccgg tattaacttt
     2461 gtcgccaacc cgctggtcaa tattcatctg caaggacgat tcgatacgta tccaaaacgt
     2521 cgcggcatca cgcgcgttaa agagatgctg gagtccggca ttaacgtctg ctttggtcac
     2581 gatgatgtct tcgatccgtg gtatccgctg ggaacggcga atatgctgca agtgctgcat
     2641 atggggctgc atgtttgcca gctgatgggc tatgggcaga ttaacgatgg cctgaattta
     2701 atcacccacc acagcgccag gacgttgaat ttgcaggatt acagcattgc cgccggaaac
     2761 agcgccaacc tgattatcct gccggctgaa aatggatttg atgcgctgcg ccgtcaggtt
     2821 ccggtacgtt attcggtacg tggcggcaag gtgattgcca gcacacaacc ggcacaaacc
     2881 accgtatatc tggagcagcc ggaagccatc gattacaaac ggtgatcggc tgggctgtgt
     2941 gttgacaaag cgagcgtagt ttatgccgga tgcggcttga acgccttatc aggcctacgt
     3001 agagcactga actcgtaggc ctgataagcg taacgcatca ggcaattgtg tcagtggtta
     3061 ccgcgattca ttctcgccaa catccgcaca ttcctccaac gccatgtgca aaaatgcctt
     3121 cgcggcggct gtctgccagc tatttttccg ccgcaacaaa accgccgttc tctccagtag
     3181 tggcggggca agaggaatcg ctttaagccc gtcatgttgt gtggcaatcg ctgctggtaa
     3241 caatgtggaa agggaagtgc ggcgaatcag ctccagaacc gcgctaattg agttcgcctc
     3301 aatgaccacc tgtggatgca gccccgcttt ctcgcagtag tggtcaatct gctctctggt
     3361 ggcaaattcc gcgctgagca ggaccagttt ttcatcatgc aagcgactca acgccacctg
     3421 ttcacaggca gccagcggat gatgttgcgc cacgactaac gctaaacttt ctgtcagtaa
     3481 aggaattgcc tccagctccg gcaaatgcac aggcgcgaag gcaatcccaa cgtccagctc
     3541 gtcgcgacaa agcagatctt cgattttctc ctgcgacatt tcctgtagct ggagcgtgat
     3601 gccggggtag cgcgcataga aatccgccat taaggggccg ataaagtagc tcgtaaaggt
     3661 gggggtgacg gcgatacgca gcgatcctct cgtcagatcg gcaacatcat gaatcgcccg
     3721 tttacccgct cccagttcct gtaacgcccg gctggcgtac tgtcgccaga cttctcctgc
     3781 atcagtgaga tgaatcgttc gcccgctacg gtcaaacagc ggcacgccta aactctcctc
     3841 taactggcga atctgctggg aaagcgcagg ctgagagacg tgcaacgcac tggcggcacg
     3901 ggtgaagctg ccatgttcag ccacggcaag aaaataattg atatgtcgag agagcattcg
     3961 ccaacctata agtaaattca atagaactca tcataaatga gacttttacc ttatgacaat
     4021 cggcgagtag tctgcctctc attccagaga cagatagagg ttaacggtga aagagattat
     4081 tgatggattc cttaaattcc agcgcgaggc atttccgaag cgggaagcct tatttaaaca
     4141 gttggcgaca cagcaaagcc cgcgcacact ttttatctcc tgctccgaca gccgtctggt
     4201 ccctgagctg gtgacgcaac gtgagcctgg cgatcttttc gttattcgca atgcgggcaa
     4261 tatcgtccct tcctacgggc cggaacccgg tggcgtttct gcttcggtgg agtatgccgt
     4321 cgctgcgctt cgggtatctg acattgtgat ttgcggtcat tccaactgtg gcgcgatgac
     4381 cgccattgcc agctgtcagt gcatggacca tatgcctgcc gtctcccact ggctgcgtta
     4441 tgccgattca gcccgcgtcg ttaatgaggc gcgcccgcat tccgatttac cgtcaaaagc
     4501 tgcggcgatg gtacgtgaaa acgtcattgc tcagttggct aatttgcaaa ctcatccatc
     4561 ggtgcgcctg gcgctcgaag aggggcggat cgccctgcac ggctgggtct acgacattga
     4621 aagcggcagc atcgcagcgt ttgacggcgc aacccgccag tttgtgccac tggcagctaa
     4681 tcctcgcgtt tgtgccatac cgctacgcca accgaccgca gcgtaacctt atttttaaac
     4741 catcaggaga tgcaccatga ttcagtcgca aattaaccgc aatattcgtc ttgatcttgc
     4801 cgatgccatt ttgctcagca aagctaaaaa agatctctca tttgccgaga ttgccgacgg
     4861 caccgggctg gcagaagcct ttgtgaccgc ggctttgctg ggtcagcagg cgcttcctgc
     4921 cgacgccgcc cgccaggtcg gggcgaagct ggatctcgac gaagacgcca ttctactgtt
     4981 acagatgatc ccactgcgtg gctgcattga tgaccgtatt ccaaccgacc caacgatgta
     5041 tcgtttctat gaaatgttgc aggtgtacgg cacaaccctg aaagcgttgg ttcatgagaa
     5101 atttggcgat ggcattatca gcgcgattaa cttcaaactc gacgttaaga aagtggcgga
     5161 cccggaaggt ggcgaacgtg cggtcatcac cttagatggt aaatatctgc cgaccaaacc
     5221 gttctgacag ccatgcgtaa ccatcaaaag acgttcacga tgctgctggt tctggtgctg
     5281 attggtctta atatgcgacc actgctcacc tccgtcgggc cactgctacc gcaattgcgc
     5341 caggcaagcg gaatgagctt tagcgtggct gccctgttga ccgctctgcc ggtggttacc
     5401 atgggcgggc tggcgctggc cggaagctgg cttcatcagc atgtcagcga acgtcgcagc
     5461 gtcgccatca gtttgttact gattgccgtc ggtgcattga tgcgtgagct ttaccctcaa
     5521 agtgcgctgc tgcttagcag cgcactgctc ggtggggtgg ggatcggcat cattcaggcg
     5581 gtgatgcctt cggtgattaa acggcggttt cagcagcgca cgccactggt gatggggctg
     5641 tggtccgcgg ctctgatggg cggcggtggg cttggtgccg ccctgacacc ctggttagtt
     5701 caacatagcg aaacctggta tcaaacactc gcctggtggg cgctgcctgc cgttgttgcg
     5761 ctctttgcct ggtggtggca aagcacccgc gaggtcgcct cttcccacaa gacaacaacc
     5821 attccggttc gcgtggtttt cactcctcgc gcgtggacgc tgggtgttta cttcggtctg
     5881 attaacggtg gttacgccag cctgattgcc tggttacccg ccttctatat tgagattggt
     5941 gccagcgcgc agtacagcgg ttccttgctg gcattgatga cgcttgggca agccgcagga
     6001 gctttgctga tgcctgctat ggctcgccat caggatcggc gcaaactgtt aatgctggcg
     6061 ctggcgttac aactggtggg tttctgcggc tttatctggc tgccgctgca attgccggta
     6121 ttgtgggcga tggtgtgtgg tttaggtctg ggcggcgcat ttccgctctg tttgctgctg
     6181 gcgctcgatc actctgtgca accggctatt gctggcaagc tggtggcgtt tatgcaggga
     6241 atcggtttta tcatcgccgg gcttgccccg tggttttctg gcgtgctgcg tagtatcagg
     6301 ggcaattact tgatggactg ggcgtttcat gcgctgtgcg tcgtagggct gatgattata
     6361 accctgcgtt ttgcaccagc acgttttccg cagctgtggg tcaaagaggc atgatgcgac
     6421 gcttgtttct gcgctttgtt cacgccggat g
//
LOCUS       DBFIFI010000230         6328 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.230, whole genome shotgun sequence.
ACCESSION   DBFIFI010000230 DBFIFI010000000
VERSION     DBFIFI010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.230"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(248..1384)
                     /locus_tag="N7B39_004228"
     CDS             complement(248..1384)
                     /locus_tag="N7B39_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HGU2424094.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(1387..1749)
                     /locus_tag="N7B39_004229"
     CDS             complement(1387..1749)
                     /locus_tag="N7B39_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HGU2424095.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIASLSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            2286..4199
                     /gene="mtlA"
                     /locus_tag="N7B39_004230"
     CDS             2286..4199
                     /gene="mtlA"
                     /locus_tag="N7B39_004230"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HGU2424096.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVETLSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            4294..5442
                     /gene="mtlD"
                     /locus_tag="N7B39_004231"
     CDS             4294..5442
                     /gene="mtlD"
                     /locus_tag="N7B39_004231"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HGU2424097.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVDAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISDLDANSEVVSEAVTAYKAMQ"
     gene            5442..6029
                     /gene="mtlR"
                     /locus_tag="N7B39_004232"
     CDS             5442..6029
                     /gene="mtlR"
                     /locus_tag="N7B39_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HGU2424098.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(6041..6250)
                     /gene="yibT"
                     /locus_tag="N7B39_004233"
     CDS             complement(6041..6250)
                     /gene="yibT"
                     /locus_tag="N7B39_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HGU2424099.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
BASE COUNT         1575 a         1612 c         1629 g         1512 t
ORIGIN      
        1 gcactcctgc tcttttttga ttagagccgt cgcaggagga acaactcata tcatcattgc
       61 gatctcgacg atgtatagtc tcatcctgcg gcagaacaag acggataaaa aatccaatga
      121 gagcacactc atccagaact tattaacata atgaatatta aagaaaaatc atcgcagcta
      181 atgaagtaat atgcaaaaat aattagtcct tacaaggagc aaagttccat aatctccccc
      241 ctccccctca atgatccaaa taaagataat gcatccagct ggtcattctt agcagcactt
      301 tccgcatcac cgaaacatgg ctgaaatggt cggagtaaac cgccacctgg gcgtagatgc
      361 cgtcgggtaa ggcatcgata tcatcgttag ggtccagttc aatggttccc agcacaccgt
      421 ccgtgccggg cacgaccgtt aatgattgca ataccccctg cgcctgataa gaaccgcctg
      481 gcacgacagg taaaatacta gtcagtttgc cgtgaaacac ctgcccaggt agcgcgttaa
      541 acaccacttc cgcatcatcg ccgggtttca gacgtaacag cgagttttgc cgaaattggg
      601 cgacaatttg ccgtttttgc tcggggatga agaccatcac cggacgcagc ggcaaggcag
      661 ctgcgtatgt acctgggcgg atcagtacct gagtaacgta gccattgctc ggcgcgcgaa
      721 tgacagtctg ctcaaggtta tattttgctt cagtaagttg cgctcttaag ctcacaatct
      781 gcgattgctc gccgttaacc atactgtcga gctggctctg gatctgcgcc tgctccgcca
      841 ccgagccttt caccagcgca tcctgcgcga ggaaattttg ccgcgcatcg tcgatgtcac
      901 gttccgagaa cggattcacc gccgcctggc tgcctttcaa gtaacgttga taatttttaa
      961 acagacggtc gcgctccgct gaaacctggg tggtgttggc ctgcgcttca gtgagctgcg
     1021 cacgcagcgt ctttatatta tgcgtcgccg tcatcaggtc agcctgaagt ctgtcaactc
     1081 gcgcctggta acgaaccggg tcgagcttaa aaagcacctc gcccttttga ataagctgat
     1141 tattcttgtc agtgacttca gtaacaattc ccgtcacctg tggcgtgata gggatcgcta
     1201 tcactgcctt ttgcgcggta aaagtgtaag ggtggttgta gttcatcaat aaaatcaaac
     1261 cactcaccag aaacacgcct cccagcgccg ccgtcgccag cgtccactga tttaccggaa
     1321 tgcggaagat tttaaagacc gcccacgcca gcgccacgta agttaaaaca atcaatagat
     1381 ccataattag atctccggga acgtggaata gtcagtcttt ttctcggcgg cgagttgatg
     1441 ctccagccgg gaaattcggt cagaaagtga ggcgatttcc gggtcagtcg ctttctcctg
     1501 cgacgcaaca tgtgactgca taccccagcc acgctccggt tgatagagcg tcgcccagat
     1561 ccacagaaac ggccagataa catgcagagt aaacaggctc acccagcccg ccgtatgaat
     1621 agcgtcggca tgggggtgat tgcgcttttt ggcaatcaga taagggatgt catgtatcgc
     1681 gatgattcca taaaaaatca ccagaaagac gaagatcagc actcccaacg cgaaatagtt
     1741 tagaaacata tctgcctcgg attcacgttt atcagtgttg tttttgggct ggcagccaga
     1801 agggagtcag gctgatattt tgacaataat ccgggttcgc gattctcgcc ataacaccaa
     1861 agaataattt ttagaggtga tgagttgctt agttacataa cgattgtatg acgaaggcat
     1921 aacatgctgt agatcacatc aggtgaacgc cgtaagaaaa tatcttgtga ttcagatcac
     1981 aaagattcaa caaaccatca aaacaaaaat gtgacactac tcacatttaa atgccatttt
     2041 tagcgaaaat cgccgccttg ttgctttttt acacaagcgt tttgtgatga acgtcacgtc
     2101 aattacctct ctacccccta tatttatgtg attgatatca cacaaaaggc cgtcgactgg
     2161 acagttaacc gattcagtgc cagatttcgc agtatctaca aggtccggct acctctgccg
     2221 ccacattaac aaaaaacctc gggcttccag cctgcgcgac agcaaacata agaaggggtg
     2281 tttttatgtc atccgatatt aagatcaaag tgcaaagctt tggtcgtttc ctcagcaaca
     2341 tggtgatgcc aaatatcggc gcgtttatcg cgtggggtat catcaccgcg ttatttattc
     2401 caacagggtg gttaccgaac gagacgctgg cgaagctggt cgggccgatg atcacttatc
     2461 tcctgccgct gctgatcggt tataccggtg gtaagctggt aggcggcgaa cgtggcggcg
     2521 tagtcggtgc catcaccacc atgggcgtta tcgtcggcgc agacatgccg atgttcctcg
     2581 gttctatgat tgcaggtccg ctgggcggct ggtgcattaa gcacttcgac cgctgggtag
     2641 acggtaagat caaatccggt tttgagatgc tggtgaataa cttctccgca ggcatcatcg
     2701 ggatgatcct cgctattctg gcattcctcg gcattggccc gattgttgaa accctgtcca
     2761 aaatgctggc tgcgggcgtt aacttcatgg ttgtccatga catgctgccg ctggcgtcta
     2821 tctttgttga accggcgaaa atcctgttcc tcaacaacgc cattaaccac ggtatcttct
     2881 cgccgctggg tattcagcag tcccatgaac tgggtaaatc aatcttcttc ctgattgaag
     2941 ctaacccagg tccaggtatg ggcgtgctgc tggcgtacat gttctttggt cgtggtagcg
     3001 ctaaacagtc tgcgggcggt gcggcaatca tccacttcct gggtggtatc cacgaaatct
     3061 acttcccgta tgtgctgatg aatccgcgtc tgatcctcgc agtcatcctc ggcggtatga
     3121 ctggcgtgtt cacgctgact atcctgggcg gtggtctggt ttctccggca tctccgggtt
     3181 ctatccttgc tgtactggcg atgacaccaa aaggtgctta tttcgctaac atcgcgggtg
     3241 tgtgtgcggc gatggctgtc tccttcgttg tctctgctat tttgctgaaa accagcaaag
     3301 tgaaagaaga agatgatatt gaagcagcaa ctcgtcgtat gcaggacatg aaagctgagt
     3361 ctaaaggtgc atctccgctg tctgcgggcg atgtgactaa cgacctgagc cacgtacgta
     3421 aaatcatcgt tgcctgtgac gccggtatgg gttccagtgc gatgggcgca ggcgtgctgc
     3481 gtaagaaaat tcaggatgca ggtctgtcac agatttcggt cactaacagc gcgatcaaca
     3541 acctgccgcc agatgtggac ctcgtcatca ctcaccgtga cctgaccgaa cgcgctatgc
     3601 gccaggttcc tcaggcacag catatttcgc taaccaactt cctcgacagc ggcctgtaca
     3661 ccagcctgac tgaacgtctg gttgctgccc agcgacatac tgaaaacgaa gtgaaagtga
     3721 aagacagtct gaaagacagc tttgacgatt ccagtgctaa cctgttcaaa ctgggcgcgg
     3781 agaacatctt cctcggtcgc aaagcagcaa ccaaagaaga agcgattcgt tttgctggcg
     3841 agcagctggt gaaaggcggt tacgttgagc cggagtacgt tcaggcgatg ctggatcgtg
     3901 aaaaactgac cccgacttat ctgggtgagt ctatcgcggt gccacacggt acggttgaag
     3961 cgaaagatcg cgtactgaaa acgggcgtgg tgttctgcca gtacccggaa ggcgtgcgct
     4021 tcggtgaaga agaagatgac attgctcgtc tggtgattgg tattgctgcc cgtaacaacg
     4081 agcacattca ggttatcacc agcctgacca acgcactgga tgatgagtcc gtcatcgagc
     4141 gtctggcaca caccaccagc gtggatgaag tgctggaact gctggcaggt cgtaagtaat
     4201 ccaatcctac cctctcctca cggagagggt ttgggtgagg gaaaagcctc accccagccc
     4261 tctcgggtaa aaacattgat gaaggttaat actatgaaag cattacattt tggcgcaggt
     4321 aatatcggtc gtggctttat cggtaaactg ctggcagacg cgggtatcca actgacgttt
     4381 gccgatgtca atcaggtggt acttgatgcc ctgaatgccc gtcatagcta tcaggttcat
     4441 gtggtcggtg aaaccgagca ggtagatacc gtttccggcg tcgatgctgt cagcagcatt
     4501 ggtgatgatg tcgttgatct gattgctcag gtcgatttag tcactaccgc cgttggcccg
     4561 gttgtgctgg aacgtattgc tccggctatc gccaaagggc tggtgaaacg taaagaacaa
     4621 ggtaatgaat ccccgctgaa catcatcgcc tgtgaaaaca tggtacgcgg taccacgcag
     4681 ctgaaaggtc atgtgatgaa cgccctacca gaagacgcca aagcgtgggt agaagaacac
     4741 gttggctttg tcgattccgc cgttgaccgc atcgtaccgc cttcggcttc ggcaactaac
     4801 gatccgctgg aagtgacggt agaaactttc agcgaatgga ttgtcgataa aacgcagttc
     4861 aaaggcgcac tgccgaacat cccaggcatg gagttaaccg acaacctgat ggcatttgtc
     4921 gaacgtaaac tcttcaccct gaacacgggt catgctataa ccgcgtacct cggaaaactg
     4981 gccggtcatc agaccattcg tgacgctatt ctcgacgaga aaatccgcgc ggtggtaaaa
     5041 ggtgcgatgg aagaaagtgg tgcggtactg atcaagcgct acggctttga cgcagacaaa
     5101 catgcggcgt acatccagaa aatcctcggt cgttttgaga acccgtatct gaaagatgat
     5161 gtagagcgcg taggccgtca gccgctgcgt aaactgagtg ctggcgaccg tctgatcaag
     5221 ccattgctcg gtacgctgga atacagcctt ccgcacaaga atctgattca ggggattgct
     5281 ggtgcaatgc acttccgcag tgaagacgat ccgcaggctc aggaactggc agcactgatc
     5341 gctgacaaag gtccgcaggc ggcgctggca cagatttccg atcttgatgc caacagcgag
     5401 gttgtatccg aggcggtaac cgcttataaa gcaatgcaat aatggtggac caggcgcagg
     5461 acaccctgcg cccgaataac agattgtcag atatgcaggc aacaatggaa caaacccagg
     5521 cctttgaaaa ccgtgtgctt gagcgtctga atgctggcaa aaccgtgcga agctttctga
     5581 tcaccgccgt cgagcttttg accgaggcgg taaatcttct ggtgcttcag gtattccgca
     5641 aagacgatta cgcggtgaag tatgctgtag aaccgttact cgacggcgac ggtccgctgg
     5701 gcgatctttc tgtgcgttta aaactcattt acgggttggg cgtcattaac cgccaggaat
     5761 acgaagatgc ggagctgctg atggcattgc gtgaagaact aaatcacgac ggcaacgagt
     5821 acgcctttac cgacgacgag atccttggac cctttgggga actgcactgc gtggcggcgt
     5881 taccaccgcc gccacagttt gaaccagcag actccagttt gtatgcaatg caaattcagc
     5941 gctatcaaca ggctgtgcga tcaacaatgg tcctttcact gactgagctg atttccagaa
     6001 tcagcttaaa aaaagccttt caaaagtaag caacgtctgc ttactgcccc tctacctgct
     6061 tcggccgata aagccgacga taatactcca gacgttgtag atataacggc acgctttcat
     6121 cgggtattcc ggacggaatc gcgttacggg gaggaagttt tttcagatac tcccggaacg
     6181 cctggcttga tgccatgaaa tctacggctt tatcgataag aagcggaacg ttttcaccta
     6241 atttgcccat gatgatatct ccgtattacc ccgcgccggg aatgcgcggc cgccaatttt
     6301 agtttaggtt cccggtaaag atgacatt
//
LOCUS       DBFIFI010000231         6289 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.231, whole genome shotgun sequence.
ACCESSION   DBFIFI010000231 DBFIFI010000000
VERSION     DBFIFI010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6289
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.231"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            1..4520
                     /locus_tag="N7B39_004234"
                     /pseudo
     CDS             1..4520
                     /locus_tag="N7B39_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ECSE_1600 family autotransporter"
     gene            4636..5700
                     /gene="hipA"
                     /locus_tag="N7B39_004235"
                     /pseudo
     CDS             4636..5700
                     /gene="hipA"
                     /locus_tag="N7B39_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
     gene            5971..6240
                     /locus_tag="N7B39_004236"
                     /pseudo
     CDS             5971..6240
                     /locus_tag="N7B39_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         1690 a         1370 c         1775 g         1454 t
ORIGIN      
        1 aatatttcca ataatggact aattaataac aaagaatatt cattggtggg cgttcaggac
       61 ggttcccacg gtgtcgtcaa cgtgaccgat aaagggcatt ggaatttcct cggaacgggc
      121 gaagctttcc gctatatcta tatcggtgat gctggcgacg gtgaacttaa tgtctcgcgt
      181 gaaggtaaag tagattcggg aattatcact gcggggatga aagaaacagg cacaggcaac
      241 cttactgtta aggataagaa ctccgttatc actaatctcg gaactaatct tggttatgac
      301 ggccacggcg aaatgaatat cagtaatgag gggcttgttg tcagcaacgg aggaagctct
      361 ctcggttatg gagaaaccgg cgtcgggaag gttagcatca ccacgggggg gatatgggag
      421 gtcaataaga atgtctatac caccattggt gttgcgggcg tcggaaacct caatattagc
      481 gatggcggta agttcgtatc gcaaaatatt acttttttgg gcgataaagc aagcggtatc
      541 ggcacactga acctgatgga tgcgacatcg tcgttcgata ctgtgggtat caatgtcggt
      601 aattttggta gcggtatcgt aaatgtcagt aatggtgcca cccttaattc aacgggctat
      661 ggatttatcg gaggaaatgc ctccggtaag ggaatagtta atatttcaac ggatagtctc
      721 tggaatttaa agacgtcttc tactaacgcc caattgctac aggtcggtgt attaggcacg
      781 ggtgaactga atattaccac cggaggtata gttaaagcgc gtgatacaca gatagctctc
      841 aatgacaaaa gtaagggcga cgtgagggtg gatgggcaga actctcttct tgaaacattc
      901 aatatgtacg tagggacatc tggtacgggt acgttaaccc tgacgaatag cggcacgctg
      961 aatgtcgaag gtggagaagt ttacttaggt gtttttgaac ctgctgtagg aacgctaaac
     1021 attggtgcag ctcacggtga ggcggcggca gatgccggat ttatcaccaa cgcgacgaaa
     1081 gtagagtttg gctctggcga aggtgttttt gtctttaatc atactaataa cagtgatgcc
     1141 ggctaccagg tcgatatgct gattacaggt gacgataaag acggaaaagt gatccatgat
     1201 gcaggccata cggtgttcaa tgcagggaat acttatagcg gtaaaacgct ggtcaatgac
     1261 ggcctcctga ccattgcgtc tcatacggca gatggggtaa cgggcatggg gtcgagtgaa
     1321 gtaaccattg caagccccgg tacgctcgac attctcgcat caacgaacag tgcaggagat
     1381 tacacgctga ccaatgcgct caaaggcgat ggcttgatgc gagttcagct gtcatcctcc
     1441 gacaagatgt ttggctttac acatgcaaca gggactgaat tcgccggtgt tgcccaactg
     1501 aaagacagta ccttcactct ggaacgcgac aacaccgctg cgcttactca cgcgatgttg
     1561 cagtctgaca ttgaaaatac cacatcggta aacgtgggag agcaatccat tggtggactg
     1621 gccatgaatg gcggtacgct cattttcgat acggatattc ctgctgcgac gcttgcagag
     1681 ggatatatca gcgtcgatac gctggttgtc ggcgcgagtg actacacctg gaaaggccgt
     1741 aactatcagg taaacgggac gggcgacgtg cttatcggcg tgcctaaacc gtggaatgat
     1801 cctatggcga ataaccctct gacgacgctc aatttgctgg aacacgatga taaccatgtc
     1861 ggcgttcaac tggtgaaggc gcaaacggtt attgggtcgg gtggctcatt aacgttacgt
     1921 gatttacagg gcgacgaggt ggaagcggac aaaacgttac acattgcgca aaacggaacg
     1981 gtggtcgccg agggtgatta tggattccgc ctcacgaccg caccaggtga tggtttgtac
     2041 gttaactatg ggctgaaagc gctgaacatc catggagggc aaaagctgac gttagccgaa
     2101 catggcggag cctatggcgc aacggccgat atgtcggcaa aaatcggtgg tgaaggggat
     2161 ctggcaatca atacggtgcg acaggtttcg ctttccaacg gtcagaacga ctatcagggg
     2221 gcaacctacg ttcagatggg gacattacgt accgatgcgg atggcgcgct gggcaacacc
     2281 cgggaactga acatcagcaa cgcagccatc gtcgatctta atggatcgac gcagacggta
     2341 gagacattca ccgggcagat gggttcgact gttttgttca aagaagggtc gctgacggta
     2401 aataaaggtg ggatcagtca gggtgaactg acaggtggcg gaaacctgaa tgttacaggg
     2461 ggaacgctgg ctgtcgaggg gcttaatgca cgctacaatg cgttaaccag cgttagccca
     2521 aatgcggaag tcagcctcga taatacgcag gggttaggca gaggaaatat tgccaatgac
     2581 ggtctgttaa cgctaaaaaa cgtgactggc gaactgcgta atagcataag cgggaagggt
     2641 atcgtgagcg caaccgccag gacagatgta gagctggatg gcgataatag ccgctttgtg
     2701 gggcaattca acattgatac aggcagcgcg ctcagcgtca acgagcagaa aaacctgggt
     2761 gatgcttccg ttatcaataa tggcctgctc accatctcca ctgagcgtag ctgggcgatg
     2821 acgcacagta tcagcggtag cggtgatttg acaaaactgg gtaccgggat cctgactctt
     2881 aacaacgatt cctcggcgta tcagggtacg acggatatcg tgggggggga aattgctttc
     2941 ggttccgact ctgccattaa tacggcaagt caacacatta atatccataa cagcggtgtg
     3001 atgtcgggaa atgtcaccac tgcaggtgat gtgaacgtta tgtctggggg gacactgcgt
     3061 gtcgctaaaa ccacaatcgg cgaatcggcg gcaacttgga gaatggcggc acggttcaaa
     3121 tgaacagtga aggaggaaaa ccggggaatg tactgaccgt taacggcaac tataccggaa
     3181 acaatggcct gatgacgttc aacgcgacgc tgggcggcga taattcaccc accgataaga
     3241 tgaacgtgaa aggcgatacc caagggaaca ctcgcgttcg ggttgataac attggcggcg
     3301 tcggtgcgca aacggtcaac ggtattgaac tcattgaggt tggcggtaat tctgcaggta
     3361 atttcgcgct gaccaccgga actgtcgaag ctggggctta cgtctacacg ctggctaaag
     3421 ggaaggggaa tgacgagaaa aactggtatc tgaccagtaa atgggacggc gtaacgccag
     3481 cggatacacc cgatcccatc aataatcccc ctgttgtgga tccggaaggc ccatcagttt
     3541 atcgcccgga ggccggaagc tatatcagca acattgccgc agccaactcg ctgtttagcc
     3601 atcgcttaca cgaccgtctg ggtgagccgc aatatacaga ttcactgcat tctcaggatt
     3661 cagcaagcag tatgtggatg cgtcatgtcg gggggcacga acgttccagt gccggagacg
     3721 gccagctaaa tactcaggct aaccgctatg tattgcagct aggcggcgat ttggcgcagt
     3781 ggagtagcaa cgcgcaggat cgctggcatc ttggcgtgat ggcaggctac gccaatcagc
     3841 acagtaatac tcagagtaat cgtgtgggtt ataaatcgga tgggcgcatc agcggttaca
     3901 gcgctgggct gtacgcgacc tggtatcaga acgatgcgaa taagaccggc gcttatgttg
     3961 acagctgggc gctgtataac tggtttgata acagcgtcag ttccgataac cgttctgctg
     4021 acgactatga ttctcgcggt gtgacggcct ctgttgaggg tgggtatacc tttgaagcgg
     4081 gaacatgtag cggcagcgaa gggacgctga atacctggta cgtccagcca caggcgcaaa
     4141 tcacctggat gggtgtgaaa gattctgacc atgcccggaa agacggaacg cgcattgaaa
     4201 cggaaggcga cggaaacgtg caaacgcgac ttggggtgaa aacctacctg aatagccatc
     4261 accagcgtga cgatggtaaa cagcgtgagt tccagcctta cattgaagcg aactggatca
     4321 acaatagcaa agtctacgcc gtgaagatga atggtcaaac cgtaagccgt gatggtgcgc
     4381 gaaatctcgg tgaagtacgt accggggttg aggcgaaagt aaataacaac cttagcctgt
     4441 gggggaatgt cggtgtgcaa ctaggtgata aaggctatag cgatactcag ggcatgctgg
     4501 gagtgaaata tagctggtaa acagtataag ccgcatgtcg agatggcatg cggcttaata
     4561 ttgccaactt caaaaggctt ttaaagcctc ccttttatcc gcgatcgcgg atatcgcagc
     4621 gtttatcccg tacagtttga tttattgtca gaaatagggc gagacagcgt tggtgccgtg
     4681 acgttaatac ccgaagacga aaccgtaacg cgtccgataa tggcatggga aaagcttact
     4741 gaagtcagac ttgaagaagt attaacggct tataaagcag atatcccgct aggcatgatt
     4801 agagaagaaa atgactttcg catctcggtt gctggcgcac aggagaagac agcactgctc
     4861 agaataggca atgactggtg cattccgaaa ggaataacgc cgacgacgca catcattaaa
     4921 ttaccgattg gggaaatcag gcagcccaat gcgacgctcg atctcagcca aagcgttgat
     4981 aatgagtatt actgtctgct gctggcgaaa gaacttgggt tgaatgtccc ggacgcagaa
     5041 atcattaaag cgggaaatgt gcgcgcgtta gcggtcgaac gttttgacag gcgttggaat
     5101 gctgagcgaa cggttttact tcgcttgcca caggaggata tgtgtcagac attcggttta
     5161 ccttcatcgg tgaaatatga atcagatgga ggcccaggca tcgcgcggat catggctttt
     5221 ttgatggggt tcagcgaggc gcttcgcgat cgttatgatt ttatgaaatt ccaggtcttc
     5281 cagtggttga ttggcgcaac ggatggccat gcaaaaaact tctccgtatt tattcaggct
     5341 ggcggcagtt atcgactcac gccattttac gacatcattt cagcatttcc ggtccttggc
     5401 ggtacgggaa tacacatcag cgatctcaaa ctggcaatgg ggcttaacgc atccaaaggc
     5461 aaaaaaacgg caatcgataa aatttatccg cgacattttt tggcgacagc aaaggtgctg
     5521 agattcccgg aagtgcagat gcatgaaatc ctgagtgact ttgccagaat gattccggca
     5581 gcactggata acgtgaagac ttcattaccg acagattttc cagagaacgt ggtgacggca
     5641 gttgaaacca atgtgttgag gttgcacggt cggttaagcc gagaatacgg tattaagtaa
     5701 gagtaagata tggggtttgg tcgttgttaa tgagcaggac aatcatgacc gccaacgact
     5761 agattctccc ttggtttata ttggtgcgca taatgtatat tatgttaaat cgtatctgag
     5821 tgatacgaag tgattttccg gtagtcgtta ttcgttcagg attccagaac ttaagcttct
     5881 cctctttcat aattggcctg tgcatgacgg atataggctg gctggtatgc cgatttgccg
     5941 tttgccgcat cttcccagcg ttgaagactc aagctggcaa accgcgtgca aatgagcacg
     6001 ccctctttcc atcaacactt caggcagctt gcgggaatga gtccactccg gtatcagaaa
     6061 tggctacgtc tcaatgaagt caggcgaacg atgctgaatg aacattacga tgtcaccaca
     6121 gcagcctatg ctgtcggtta cgaaagctta tcccatttcc gacgggaata tttgcggatg
     6181 tttggagagt cacctaagag agatattacc aggttaagaa agtctgtcgg tcagctttaa
     6241 taaaccctgc acttatctgt ttagagcaat tcggtgttag ttacaccag
//
LOCUS       DBFIFI010000232         6212 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.232, whole genome shotgun sequence.
ACCESSION   DBFIFI010000232 DBFIFI010000000
VERSION     DBFIFI010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.232"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(38..571)
                     /locus_tag="N7B39_004237"
                     /pseudo
     CDS             complement(38..571)
                     /locus_tag="N7B39_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498860.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            complement(576..740)
                     /locus_tag="N7B39_004238"
     CDS             complement(576..740)
                     /locus_tag="N7B39_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424100.1"
                     /translation="MSGWCRPEIMILTMKVKCIGSLIGRSEAAVRMKAQVKGISLILR
                     GDFHQSTKYP"
     gene            complement(737..1417)
                     /locus_tag="N7B39_004239"
     CDS             complement(737..1417)
                     /locus_tag="N7B39_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HGU2424101.1"
                     /translation="MAARLDISREEVLNELWELKKAGFVDKSVYTWRVADNNVQQEQP
                     APEETTTATEAKISECDLTATIEQRGPQTADELATLFGTTSRKVASTLAMAISKGRLI
                     RVNQNGKIRYCIPGDNLPAEPKVESVAETDGKAFPQPAGVALPVQKDATQEDIKTETV
                     ADIVQSLPSFTATREDDLILPSLHMANRELRRAKNHVQKWERVCAALRELNKHRDMVA
                     GICRKSGQ"
     gene            complement(1505..1966)
                     /locus_tag="N7B39_004240"
     CDS             complement(1505..1966)
                     /locus_tag="N7B39_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HGU2424102.1"
                     /translation="MTEQQIRQVCRQCMDRCRAGETWPPDLAEFVALISKSGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWSQPVLYHICLEMRSKGIERQMTEGELKRLAE
                     RQLTKWAKHVSNGLSVPPVRRQLAAPKRPSGPTPIELLKQEYERRKAAGFV"
     gene            complement(1959..2999)
                     /locus_tag="N7B39_004241"
     CDS             complement(1959..2999)
                     /locus_tag="N7B39_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HGU2424103.1"
                     /translation="MSIDALRWAKKVKTGSSSSKSVLTWLADMCGADLCAYPSVSALA
                     EVTELNKKTVQDSLRHLMEIGLIVDTGERKGRTKQIVVYRLIGVEESVAEPEYTQKRE
                     SLKVGKIGAVNKNSTENGYVSAQNRPKNRTLSCMENNQRHPNFPSKTPKNGSRNPKEP
                     KDLNPTHNARESAPTSEQEVLSLQAAPPVFLYGLSEPIGKFPMSDSWYPSRDFRRRAA
                     LWGMALPETEFTPAELAAFRDYWAAEGKVFTQIQWEQKFARHVNHVRAQVKPVSKGVN
                     HAAAPGGTASRAVQEIRAAREQWERENGFISDGNGLEAVGTHGGGLFEPLDPEERGRT
                     FEALDCTDWRDD"
     gene            complement(2971..3522)
                     /locus_tag="N7B39_004242"
     CDS             complement(2971..3522)
                     /locus_tag="N7B39_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HGU2424104.1"
                     /translation="MKITTEQVCEALDTWVCRPGMTQEQATILITEAFWALKERPNID
                     VQRVTFNDGEVDQRALGVNRVKIFERWKAIDTRDKRDKFTALIPAIMEAIRISDFRLY
                     CEITDGKSITYMIAGLNKEYGDVVESGLLFADPVVVERETDELIEKAIAFKHAYRQQY
                     QYYFADKQMSARGAYEYRCTTMG"
     gene            complement(3506..3733)
                     /locus_tag="N7B39_004243"
     CDS             complement(3506..3733)
                     /locus_tag="N7B39_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HGU2424105.1"
                     /translation="MLKIDAIAFFGSKTKLANVAGVRLASVAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRAGRLNNENHH"
     gene            3810..4217
                     /locus_tag="N7B39_004244"
     CDS             3810..4217
                     /locus_tag="N7B39_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU2424106.1"
                     /translation="MKNETFGARLLYRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPVNLTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            4481..4780
                     /locus_tag="N7B39_004245"
     CDS             4481..4780
                     /locus_tag="N7B39_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424107.1"
                     /translation="MALYIDISAIAGQVRVIRAVTKRYAPLLQKVSGECTEDIVNDFV
                     IELRGLIFSYKVTTIFADGSRETVRALRLKGCVKDLATTFWARKLDCIHNQFPLE"
     gene            4853..5071
                     /gene="ydfC"
                     /locus_tag="N7B39_004246"
     CDS             4853..5071
                     /gene="ydfC"
                     /locus_tag="N7B39_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HGU2424108.1"
                     /translation="MQKRDPVIIAPDYTDDELYEWMHQKIKAVQDLKWANEARAKQAE
                     NLSALEQDITNLEKAAALSIARMITYPR"
     gene            5094..5501
                     /locus_tag="N7B39_004247"
     CDS             5094..5501
                     /locus_tag="N7B39_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424109.1"
                     /translation="MEFKDLPTPLQEMASNIVRSQLATLDLSTAEKETIDNMVRNVRN
                     AFSGLYGSDNQKQESDVNKRVISVCVNGHVLSSIKTETATVFDCLCIVQSLVDALFRS
                     VNLENDANLRGRIIAHPYAHTLGSVDIKDPTNL"
     gene            complement(5479..5712)
                     /locus_tag="N7B39_004248"
     CDS             complement(5479..5712)
                     /locus_tag="N7B39_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424110.1"
                     /translation="MLKSTLIAKCLYQNRMVSSISIGESAVKSIFEEYFPGHDFNKWN
                     TKLPPAVSTRILKATERASTIRVNYFIKDLWDL"
BASE COUNT         1540 a         1605 c         1377 g         1690 t
ORIGIN      
        1 ataaggaaaa ttatacgcat attgaagagt ataaacctta catgttgatt acatttttgt
       61 aatcaacatc ctgtttggaa tagccagcct ttaatggata actatttctg acaatgcaat
      121 gagtataatc aagtccatct tccactgaga attagaggcg gcatgctttt tccggctctt
      181 gccggatatc cgtaattgtc cataatctgc agatttatac cttctgcatg acctgtcagc
      241 gaaaatttgt ccggtgtttc tacgggaatg acatcaaaag ttacacgcac tcgcgttacc
      301 gtgtagacct actttcctgc acttgcaaga tcacagtggt gtaaccgtaa caggaattta
      361 ttctctggac cggcagtaaa tccctgagtg gcgtggttcc catatcaatt tcccgccagg
      421 cagcctccat tgccagcgta caggctggag ccatgacctg ccctttaaat ctggcccgac
      481 catcccaccg gacgtgttct tctcccctga acttaggtac agtcatctcc agtggcacaa
      541 aagtgtcagc gccataattt ttgaccgtta tcgcgctacg gatattttgt tgactggtga
      601 aaatcacccc gcagaatcag gcttattccc ttaacctggg ctttcatcct gaccgccgcc
      661 tcactacgac caatcagact gccgatgcat tttaccttca ttgttagtat catgatttca
      721 ggcctgcacc atccgctcat tgcccggact tccgacaaat cccggcaacc atatcccggt
      781 gcttgttcag ctcccgcagc gcggcgcaga ctcgctccca cttctggaca tgattcttcg
      841 ccctacgcag ttcgcgattt gccatatgca gcgatggcaa aatcaaatca tcttctcgcg
      901 ttgcagtaaa cgatggcagc gactgcacaa tgtccgccac agtttctgtt ttaatatctt
      961 cctgtgttgc atctttctgt accggtaacg caacaccggc tggctgagga aaggctttac
     1021 catcggtttc cgctaccgat tcaactttcg gctctgctgg taaattatca cctggtatgc
     1081 agtaacgaat tttaccgttc tggtttacgc gaatcagacg acctttgctg attgccattg
     1141 ccagcgttga agcaactttg cgggatgttg taccgaacag cgtagccagt tcatccgccg
     1201 tttgtggtcc gcgttgttca atcgtcgcgg ttaaatcgca ctctgagatt ttcgcttctg
     1261 ttgccgtggt ggtttcttct ggcgctggct gttcctgctg aacgttgtta tcagccacac
     1321 gccaggtgta tacgctttta tcaacgaagc cagccttttt cagttcccac agctcgttca
     1381 gtacttcttc acgactgata tcaagtcgcg cagccagcta taccgacgtg gcttttccca
     1441 tcgctttcag tgcgtcaaaa acagtctcca taaatttcct cccggtaaaa aatcacttct
     1501 caactcaaac aaaaccagcc gctttccggc gttcatattc ctgtttcagc aactcaattg
     1561 gcgttggccc cgacgggcgt ttgggggccg ccagttgtcg ccggactggc ggaacgctca
     1621 ggccgttact aacatgcttt gcccatttcg tcagttgccg ttctgcaagc cgttttaatt
     1681 ccccttcggt catctggcgc tcaatcccct ttgaacgcat ctcgaggcaa atgtgataca
     1741 gcacaggctg agaccacggg tacttatcac ttccgtcgta tcgccaggac tcattgcgcc
     1801 agcggcggta ctcctccatc acagcatcca ccgtcagacc gaatggattg gctccgcttt
     1861 tcgaaatcag tgccacaaac tcagccaggt ccggaggcca tgtttcaccc gcccggcagc
     1921 ggtccatgca ctggcggcag acctgccgga tttgctgctc agtcatcgcg ccaatctgtg
     1981 caatccagag cttcgaaggt gcggccccgt tcttctggat ccagcggttc gaataaacca
     2041 cccccatgag ttcccacagc ttccaggccg tttccgtcgc tgataaatcc gttttcacgt
     2101 tcccactgct cacgtgctgc ccgaatttcc tgaactgccc gtgatgcggt gccacctggt
     2161 gctgctgcat ggtttacccc tttgctgact ggtttaacct gcgccctgac gtgatttacg
     2221 tgacgggcga atttctgctc ccactgaatc tgcgtaaaca ctttcccctc cgctgcccag
     2281 tagtcccgga aggcggcaag ttcagcaggt gtaaattctg tctccggcaa agccatcccc
     2341 cacaacgcag cccgtcgtcg aaaatcccgt gacggatacc agctatcgct catcgggaat
     2401 tttccgatgg gttcgctcag gccatacagg aatacagggg gggctgcctg taacgacaaa
     2461 acttcctgct cactggtcgg agcactctcg cgtgcgttat gtgtggggtt tagatctttg
     2521 ggttcctttg ggttccgtga tccgtttttg ggtgtctttg atggaaaatt tgggtgtctt
     2581 tggttatttt ccatgcagct aagagttctg tttttgggtc tgttttgtgc tgaaacataa
     2641 ccattttcgg tactgttttt attaacagca ccaattttac ccacctttaa agactcccgt
     2701 ttttgggtgt attcaggctc ggcaacactt tcttctacac cgataagtcg gtacaccaca
     2761 atttgctttg ttctgccttt tctctcaccg gtatcaacaa ttaacccaat ctccatcagg
     2821 tgtcgtaagc tgtcctgcac agtctttttg ttcagttccg ttacttctgc cagtgcagat
     2881 acagacgggt atgcacacaa atcggcaccg cacatatcag caagccaggt caatactgac
     2941 ttactggatg aactgccggt tttcaccttt ttagcccatc gtagtgcatc gatactcata
     3001 cgcccccctg gcagacattt gtttatctgc aaagtaatat tggtattgct gacgatacgc
     3061 gtgcttgaaa gcaatagctt tttctataag ctcgtcagtc tcacgttcca caacaactgg
     3121 atccgcaaaa agcagcccgg actccaccac atcgccatat tctttgttta acccggcgat
     3181 catgtacgta atgctttttc cgtcagtaat ttcacaatac aatctgaaat cgctgatccg
     3241 gatagcctcc ataattgccg gaatcagcgc cgtgaatttg tcacgcttat ctctggtgtc
     3301 gatagctttc cagcgttcga atatcttcac ccggttaacg cccagcgccc gttgatcaac
     3361 ctcgccatca ttaaacgtga cgcgttgaac atcgatgttc gggcgttctt tcagagccca
     3421 gaatgcttcc gtgattaata tcgtcgcctg ctcctgtgtc attcctggtc ggcataccca
     3481 ggtatccaga gcctcacaaa cctgttcagt ggtgattttc attgttcaac cgccccgccc
     3541 gctttgcctt acgatattcg tcataaactt tggggtcgta ctgaagttcc ccgccggatg
     3601 cctcttgcag gcgcatcgcg cgaccttcag gaaccagttc cccccatgca gcaacgcttg
     3661 ccagcctaac tcctgcgaca ttggcaagct ttgttttgct gccaaaaaac gctatagcat
     3721 caattttcaa catatcgaaa cccttagatt ttcctaagga aactagatcg tagagaaacc
     3781 taagtcaaga aaaattagaa ttacctaata tgaaaaacga aaccttcggt gctcgcctct
     3841 tatacaggcg aaaaaaatta aaactgtctc aggccgcatt aggtaagctg gtcaaagtgg
     3901 ctcacgtaac aatttctcaa tgggaaagag atgaaacaca gccagcgggg aagagattat
     3961 tcgcactgag ccaggcgctt cagtgctcgc cgacttggct tctttttgga gatgaagata
     4021 agcaaccagg cgaaccgatc ccagataatc agccagtcaa tctgacagaa gatcaaaaag
     4081 agttgcttca actgttcgac gcactgcctg agtcagagca aaaggctctg ttgtcagaga
     4141 tgcgtgctcg agttgagaat ttcaacaaac tttttgaaga actactcaaa gctcgcaaaa
     4201 gaagcgcaaa taaataaccc cccttttttt cgcaactctc tgtaataaaa agcacaaact
     4261 ttcaaatact tgtgtttttt acatcaagaa gcttaggttt ttctacacaa aaagcttgac
     4321 cataactctt aggctattct aaattctact catcaagaca ccgcacggtg ttctcagcaa
     4381 acagttccgc tacccggcgt taaggggaag cagaggattt ctcagtgggc gagtcaaaca
     4441 tcagaatgga aggcgtccag ggatcagcaa agaaacagcg atggcgcttt atattgatat
     4501 cagcgccatt gccggacagg taagagttat cagagcggta actaagcggt atgcgccttt
     4561 acttcagaaa gtctctggtg agtgcaccga agatattgtc aacgatttcg tcatcgaact
     4621 gcgaggactc atcttcagtt acaaggtgac cacaattttt gcagatggct cccgcgaaac
     4681 tgtcagagcc ctgcggctta aaggatgtgt caaagactta gccaccacat tctgggcaag
     4741 aaaacttgat tgtattcata accaatttcc tctcgagtaa cagacccctc agaggatacc
     4801 acctcgcctg acgtggttaa aaagcaggca acgctaacca caaggagccg acatgcagaa
     4861 acgagacccc gtcatcatcg cgccagacta taccgatgat gaactttatg agtggatgca
     4921 ccagaaaatt aaggcggtgc aggacctgaa atgggccaat gaagccaggg ctaagcaggc
     4981 tgaaaatctg tccgctctgg agcaggatat caccaatctg gaaaaagcag cggcattaag
     5041 cattgccaga atgattacat acccacgtta atagctaacc aacgaggcta ataatggaat
     5101 ttaaagattt accaacgcca ttacaggaaa tggcatcgaa tatagttcgt tcacaactgg
     5161 ctactcttga ccttagtacc gcagaaaaag aaaccatcga taatatggtt cgtaatgtgc
     5221 gcaatgcttt ttctgggcta tatggttctg ataatcaaaa gcaggaaagc gatgttaata
     5281 aacgggtaat ttctgtttgc gtgaatggcc atgttctttc atcaatcaaa acagaaacgg
     5341 cgacagtctt cgattgcctt tgcattgtac agagccttgt tgatgccctg tttcgttcag
     5401 tgaatttaga aaatgatgca aatctgcgag ggcgcataat agcacatcca tatgcacata
     5461 ctttaggctc tgtggatatc aaagatccca caaatcttta atgaaatagt taacgcgaat
     5521 tgtacttgct ctttcggttg ctttcagaat acgcgttgaa actgctggcg gtaatttggt
     5581 attccattta ttaaaatcat gcccgggaaa gtactcttcg aaaatacttt taactgcaga
     5641 ctcgcctatt gaaatgctgc ttaccatgcg attttgataa aggcatttag caataagagt
     5701 tgattttaac attcaccctc ctgagggttg gtaattaagg agttctccac gggtgaagtg
     5761 gagtgcgtgc gccggacacg ggtgaacatc cggcactgac agtttactga aaggatattt
     5821 ctctgaaaag tcagagcata acgcgaaagc gcacggcgag gttgctggtt catagatagc
     5881 ctgtcgttaa attttcgtcg accgtgcgct tccggttgtg gcactccgcg aaatggcgca
     5941 gcggtaagta tggcggggtt attccttccc cgttgaggac accgggttgt caggttgacc
     6001 atacgcttaa gtgacaaccc cgctgcaacg ccctctgtta tcaattttct ggtgacgttt
     6061 ggcggcatct gtttgcccat gaactgatgt ccgccctttt taaagtgaat tttgtgatgc
     6121 agtgaatgcg gctaagcgca cgcggaacag ttaaagctaa aaacagtgtt atgggtggat
     6181 tctctgtatc cggcgttaat tgttaactgg tt
//
LOCUS       DBFIFI010000233         6201 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.233, whole genome shotgun sequence.
ACCESSION   DBFIFI010000233 DBFIFI010000000
VERSION     DBFIFI010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6201
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.233"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..1639
                     /gene="hcp"
                     /locus_tag="N7B39_004249"
                     /gene_synonym="priS"
     CDS             <1..1639
                     /gene="hcp"
                     /locus_tag="N7B39_004249"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HGU2424111.1"
                     /translation="CEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQGLSAWA
                     VKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQCLAVDA
                     NARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLKGAAAY
                     MEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSILDAGET
                     GKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLPAHGYP
                     ELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRSIVGWP
                     GVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVSREKLR
                     HIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLPRLVDA
                     GQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVKNIVTG
                     PTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            1651..2619
                     /gene="hcr"
                     /locus_tag="N7B39_004250"
     CDS             1651..2619
                     /gene="hcr"
                     /locus_tag="N7B39_004250"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HGU2424112.1"
                     /translation="MTMPTNQCPWRMQVHHIKQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLTRVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAQEFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            2752..4470
                     /gene="poxB"
                     /locus_tag="N7B39_004251"
     CDS             2752..4470
                     /gene="poxB"
                     /locus_tag="N7B39_004251"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HGU2424113.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEID
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            4507..5508
                     /gene="ltaE"
                     /locus_tag="N7B39_004252"
     CDS             4507..5508
                     /gene="ltaE"
                     /locus_tag="N7B39_004252"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HGU2424114.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKDAWEFTRERN
                     LALHVDGARIFNAVVAYGSELKELTQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMTGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            5519..>6201
                     /locus_tag="N7B39_004253"
     CDS             5519..>6201
                     /locus_tag="N7B39_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HGU2424115.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNFRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSG"
BASE COUNT         1420 a         1667 c         1733 g         1381 t
ORIGIN      
        1 atgtgaacaa actatccgta ctccggcagg aaacggctgc tcatacgcgc aggggatgtg
       61 tggtaaaacg gcggaaactt ctgaccttca ggatttactc atcgcggcgc tgcaagggct
      121 ttctgcctgg gcggtaaaag cgcgtgaata cggcatcatc aaccacgatg tcgacagctt
      181 cgcgccacgt gcctttttct caaccctgac caacgttaac ttcgattctc cgcgtattgt
      241 cggctacgct cgtgaagcga ttgccttgcg cgaggcgctg aaagcacaat gcctggctgt
      301 agatgccaac gcccgcgtcg ataacccgat ggctgacctg caactggtga gcgacgatct
      361 cggcgaactg caacgtcagg cagcagaatt tacccctaac aaagataaag cggcgattgg
      421 cgaaaacatt ctcggcctgc gtctgctgtg cctgtatggc ctgaaaggtg cggcggccta
      481 tatggaacac gcacacgttc tcggtcaata cgacaacgat atttatgccc agtatcataa
      541 aatcatggcg tggctgggga cctggcctgc cgatatgaac gcgcttcttg agtgttcaat
      601 ggaaatcggc cagatgaact tcaaagtgat gagcattctg gatgcaggcg aaaccggtaa
      661 atacggtcac ccgacgccaa cccaggtcaa cgtcaaagcg acggcgggta aatgcatcct
      721 gatttccggt cacgatctca aagatctcta caacctgctg gaacagaccg aaggcacggg
      781 cgttaatgtc tacacccacg gcgaaatgct gcctgcgcat ggctacccgg agctgcgtaa
      841 attcaagcat ctggtcggta actacggcag cggctggcag aatcagcaag tggagttcgc
      901 tcgtttccct ggccccatcg tgatgacctc gaactgcatc atcgacccaa ccgtaggcgc
      961 ttatgacgat cgtatctgga cccgcagcat cgttggctgg cctggcgtgc gtcatctgga
     1021 tggcgaagat ttctctgcgg tcattgccca ggcgcaacaa atggcaggct tcccatacag
     1081 cgaaattccg cacctgatca ccgtgggctt tggccgccag acgctgcttg gcgcagcgga
     1141 tacgctaatt gatctggtga gccgtgaaaa actgcgtcat atcttcctgc ttggtggctg
     1201 tgacggcgca cgcggcgaac gtcactactt caccgatttc gccaccagcg tgccagatga
     1261 ctgcctgatc ctgactctcg cctgtggtaa atatcgcttt aacaaacttg agtttggcga
     1321 tatcgaaggt ctgcctcgtc tggtggatgc cggtcagtgt aacgatgctt actcagcaat
     1381 tattctggcg gttacgctgg cagaaaaact gggctgcggt gtcaacgatc tgccgctgtc
     1441 gctggtgctc tcctggtttg aacagaaagc gattgtcatt ctgctgacgc tgctttctct
     1501 gggcgtgaaa aatatcgtca ccggcccgac tgcacctggt ttcctgacac cagatctgct
     1561 ggcggtgctg aacgagaaat tcggcctgcg ttctatcacc actgttgaag aagacatgaa
     1621 gcaactgttg agcgcgtaag gagtttgtcg atgacgatgc caacgaatca atgcccgtgg
     1681 cggatgcagg ttcatcacat taagcaagaa acgccggatg tgtggacgat ttccctgatt
     1741 tgccacgatt actacccata ccgcgccggg caatatgcac tggtcagcgt gcgtaactca
     1801 gcggaaacgc tgcgtgctta caccatttcc tccacgccag gcgtgagtga atatatcacc
     1861 ctgaccgtgc ggcggattga tgacggtgtc ggctcccagt ggctgacccg cgatgtaaaa
     1921 cgcggtgatt atctctggct ttcagacgcg atgggggaat ttacctgcga cgataaagca
     1981 gaagataaat tcctgttgct ggctgcaggc tgcggcgtca cgccgattat gtcgatgcgt
     2041 cgctggctgg cgaagaaccg tccacaggcc gatgtgcggg tgatctacaa cgtgcgtacg
     2101 ccgcaggatg ttattttcgc cgatgagtgg cgtaactatc cggtaacgct ggtggcggaa
     2161 aataacgtta ccgaaggctt tatcgctggt cgtctcactc gcgaactcct gactcgcgtt
     2221 cctgacttag cttcacgtac cgtgatgacc tgcggcccgg ctccgtatat ggattgggta
     2281 gagcaggaag tgaaagcact cggcgtgacg cgtttcttta aagagaaatt cttcacccca
     2341 gtagcggaag ctgcgaccag cggtctgaaa ttcaccaaac tgcaaccggc gcaagaattt
     2401 tacgctcctg ttggcaccac gctgctggag gcgctggaaa gcaataacgt tccggttgtc
     2461 gccgcctgcc gcgcgggtgt ttgcggctgc tgtaagacga aagtggtttc cggtgaatat
     2521 acggtgagca gtacaatgac gctgaccgac gccgaaatcg ctgaaggtta cgtactggcc
     2581 tgctcctgcc atccgcaggg ggatttggtt ctcgcataat cgccttatgc ccgatgatat
     2641 tcctttcatc gggctattta accgttagtg cctcctttct ctcccatccc ttccccctcc
     2701 gtcagatgaa ctaaacttgt taccgttatc acattcagga gatggagaac catgaaacaa
     2761 acggttgcag cttatatcgc caaaacactc gaatcggcag gcgtgaaacg catctgggga
     2821 gtcacgggcg actctctgaa cggtcttagc gacagtctta atcgcatggg caccatcgag
     2881 tggatgtcca cccgccatga agaagtggcg gcgtttgccg ctggcgctga agcacaactt
     2941 tcaggagaac tggcggtctg tgctggatcg tgcggccccg gcaacctgca cttaatcaac
     3001 ggcctgttcg attgccaccg caatcacgtt ccggtactgg cgattgccgc tcatattcct
     3061 tccagcgaaa ttggcagcgg ctatttccag gaaacccacc cacaagagct attccgcgaa
     3121 tgtagtcact attgcgagct ggtttccagc ccggagcaga tcccacaagt gctggcgatt
     3181 gccatgcgca aagcggtgct taaccgtggc gtttccgttg tcgtgttacc gggcgacgtg
     3241 gcgttaaaac ctgcgccaga aggggcaact acccactggt atcatgcgcc acagccagtc
     3301 gtgacgccgg aagaagaaga gttacgcaaa ctggcgcaac tgctgcgtta ttccagcaat
     3361 atcgctctga tgtgtggcag cggctgcgcg ggggcgcata aagagttagt tgagtttgcc
     3421 gggaaaatta aagcacctat tgttcatgcc ctacgcggta aagagcatgt cgaatacgat
     3481 aatccgtatg atgttggaat gaccgggtta atcggattct cgtcaggttt ccataccatg
     3541 atgaacgccg acacgttagt gctactcggt actcagttcc cctaccgtgc gttctacccg
     3601 accgatgcca aaattattca gattgatatc aacccagcca gcatcggcgc acacagcaag
     3661 gtggatatgg cgctggtcgg cgatatcaag tcgaccctgc gtgcattgct gccattggtg
     3721 gaagaaaaag ccgatcgcaa gtttctggat aaagcgctgg aagattaccg cgatgcccgc
     3781 aaagggctgg acgatttagc taaaccgagc gagaaagcca ttcacccgca atatctggcg
     3841 cagcaaatta gtcattttgc cgccgatgac gccatcttta cctgtgacgt cggcacgcca
     3901 acggtgtggg cggcgcgtta tctgaaaatg aacggcaagc gtcgcctgtt aggttcgttt
     3961 aaccacggtt cgatggctaa cgccatgccg caggcgctgg gcgcgcaggc aaccgagccg
     4021 gaacgtcagg tggtcgccat gtgcggcgat ggcggtttta gcatgttgat gggcgatttc
     4081 ctctcggtag tgcagatgaa actgccggtg aaaattatcg tctttaataa cagcgtgctg
     4141 ggctttgtgg cgatggagat gaaagccgga ggctacctga cagacggtac tgagctgcac
     4201 gacaccaact ttgcccgcat tgccgaagcg tgcggaatta cgggtattcg tgtagaaaaa
     4261 gcctctgaaa tcgatgaagc cctgcaacgc gccttctcca tcgacggtcc ggtattggtg
     4321 gatgtggtgg tcgccaaaga agagttagcc attccaccgc agatcaaact cgaacaggcc
     4381 aaaggtttca gcctgtatat gctgcgcgca atcatcagcg gacgcggtga tgaagtgatc
     4441 gaactggcga aaacgaactg gctaaggtaa aaagggtggc atttcccgtc ataataagga
     4501 catgccatga ttgatttacg cagtgatacc gtaacccgac cgagccgcgc catgctcgaa
     4561 gcgatgatgg ccgccccggt tggggacgac gtttacggag acgaccctac cgttaatgct
     4621 ctgcaggact atgcagcaga gctttccggt aaagaagccg ccatttttct gcctaccggc
     4681 actcaggcca acctggtcgc tctgctcagt cactgcgaac gcggcgaaga gtatattgtc
     4741 ggtcaggccg cgcataacta tctgtttgaa gccggtggcg cggcggtgct gggcagtatt
     4801 caaccgcaac ccatcgacgc ggctgccgac ggcacgctac cgctggataa agtggcgatg
     4861 aaaatcaaac ccgacgatat ccatttcgcc cgcaccaaat tactcagtct ggaaaacacc
     4921 cacaacggca aagtgttgcc gcgtgaatac cttaaagatg catgggaatt tacccgcgag
     4981 cgcaatctgg cgctgcatgt tgacggtgcg cgcatcttta atgccgtggt ggcttacggc
     5041 agcgaactga aagagctcac gcaatattgt gattcgttca ccatttgcct gtcgaaaggt
     5101 cttgggacgc cagtcggttc attactcgtc ggtaatcgtg attacattaa acgtgccatt
     5161 cgctggcgga aaatgacagg tggcgggatg cgccagtccg gcattctggc tgccgccggg
     5221 atgtatgcgc tgaaaaataa cgtcgcacgg ttgcaggaag atcacgacaa cgccgcctgg
     5281 atggcggagc aactgcgtga agcaggcgcg gatgtgatgc gtcaggacac taatatgctg
     5341 tttgttcgcg tcggcgaaga aaatgctgcc gcgttaggcg aatacatgaa agcgagaaac
     5401 gtactgatta acgcctcgcc gattgtccgc ctggtgaccc atcttgacgt ctcgcgcgaa
     5461 caactggcag aagtcgccgc ccactggcgc gcattcctgg cgcgttaagg agggagacgt
     5521 gccgcaacgc attttagttc ttggtgccag tggctacatt ggtcagcatc tggtgcgcac
     5581 actcagccag caagggcatc agatcctggc ggcggcacgt catgtcgaca ggcttgcaaa
     5641 gctgcaactg gcaaacgtca gttgccataa agtcgatctc agctggccgg ataaccttcc
     5701 ggccctgtta caggatatcg atacggtcta ttttctggtg catagcatgg gcgaaggcgg
     5761 cgattttatc gctcaggagc gccaggtggc tctcaacttc cgcgatgcgc tacgtgaagt
     5821 accagttaag caattaatct ttctcagttc gttgcaggcc ccgccacatg agcagtcgga
     5881 ccatctgcgc gcgcgtcagg ctacggcgga cattcttcgt gaagcgaatg taccagtgac
     5941 cgaattgcgt gccggaataa tcgttggtgc aggttcagca gcgttcgaag tcatgcgcga
     6001 tatggtctac aacctgccag tgttaacgcc gccacgctgg gtacgttcac gcaccacgcc
     6061 catcgcgctg gaaaacttgc tgcactatct ggtggcgctg ttagaccatc cagccaacga
     6121 acaccgcatc ttcgaagccg ccggaccaga ggtgctcagt tatcagcaac agtttgaaca
     6181 ttttatggcg gtgagcggta a
//
LOCUS       DBFIFI010000234         6175 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.234, whole genome shotgun sequence.
ACCESSION   DBFIFI010000234 DBFIFI010000000
VERSION     DBFIFI010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6175
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.234"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            325..1038
                     /gene="aphA"
                     /locus_tag="N7B39_004254"
     CDS             325..1038
                     /gene="aphA"
                     /locus_tag="N7B39_004254"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HGU2424116.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKNFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     SMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            1149..1565
                     /locus_tag="N7B39_004255"
     CDS             1149..1565
                     /locus_tag="N7B39_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HGU2424117.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            1569..1925
                     /locus_tag="N7B39_004256"
     CDS             1569..1925
                     /locus_tag="N7B39_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HGU2424118.1"
                     /translation="MTISELLQYCMAKPGAEQSEHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(1960..4782)
                     /gene="uvrA"
                     /locus_tag="N7B39_004257"
     CDS             complement(1960..4782)
                     /gene="uvrA"
                     /locus_tag="N7B39_004257"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HGU2424119.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLYARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            5037..5573
                     /gene="ssb1"
                     /locus_tag="N7B39_004258"
     CDS             5037..5573
                     /gene="ssb1"
                     /locus_tag="N7B39_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HGU2424120.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            complement(5672..5953)
                     /locus_tag="N7B39_004259"
     CDS             complement(5672..5953)
                     /locus_tag="N7B39_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HGU2424121.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT         1499 a         1605 c         1599 g         1472 t
ORIGIN      
        1 ataaacttcg attgtttttg taatgctgta tcattaagtt cattaaatcg tacagcagat
       61 aaatgttcta tcaaatttcg ctcatttgca gagtattcat cataataaac gtaaaattaa
      121 tgtatcctta catcgagcat taaccatttt ttacaaaaaa acgacaggaa cgtatttact
      181 aggttaaata taatcatcct gcttttcatc acaaaaaccg cagataatcc ttcctttccc
      241 ctgtagctgg cgttatggtc agatagtttt tgcaacaaat ctcacaataa aaagtttcaa
      301 catactgact atttagggaa aaatatgcgc aagatcacac aggcaatcag tgccgtttgc
      361 ttattgttcg ctctaaacag ttccgctgtt gccctggcct catctccttc accgcttaac
      421 cctgggacta acgttgccag gcttgctgaa caggcaccaa ttcattgggt ttcggtcgca
      481 caaattgaaa atagcctcgc agggcgtccg ccaatggcgg tggggtttga tatcgatgac
      541 acggtacttt tttccagtcc gggcttctgg cgcggcaaaa aaaacttctc gccagaaagc
      601 gaagattatc tgaaaaaccc tgtattctgg gaaaaaatga acaacggctg ggatgaattc
      661 agcattccaa aagaggtcgc tcgccagctg attgatatgc atgtacgccg tggtgacgcg
      721 atcttctttg tgactggtcg tagcccgacg aaaacagaaa cggtttcaaa aacgctggcg
      781 gataattttc atattcctgc caccagcatg aatccggtga tctttgcggg cgataaacca
      841 gggcaaaata caaaatcgca atggctgcag gataaaaata tccgaatttt ttatggcgat
      901 tccgataacg atattaccgc cgcacgcgat gtcggcgctc gtggtatccg cattctgcgt
      961 gcctccaatt ctacctacaa acccttgcca caagcgggtg cgtttggtga agaggtgatc
     1021 gtcaattcag aatactgaca gagcgggaga gcgtgacact ctcccgaatg ctgttttttt
     1081 aatcacacct ttatcctttc gctgtcttgc tgcaaactga ttaagagagt tttatcaagg
     1141 agcagcacat gtggtatcaa aagacgctca cgcttagcgc caaatctcgt gggtttcatc
     1201 tggtaacgga tgaaattctg aatcagctgg ctgatatgcc gcgcgttaac atcggcttac
     1261 tgcatctgtt gctgcaacat acctccgcct ctctgacact taatgagaac tgcgatccca
     1321 ccgtacgcca cgacatggag cgttttttcc tccgcaccgt tcccgacaac ggaaattatg
     1381 agcatgacta tgagggagca gacgatatgc cttctcatat caaatcctca atgctgggaa
     1441 catcgcttgt attgccggtg cataaagggc gtattcagac cggcacctgg caaggcattt
     1501 ggctggggga acatcgcatc cacggcggat cgcgtcgcat catcgcgaca ctacaagggg
     1561 agtaaaaaat gaccatttcg gagttgctac aatattgcat ggcaaaacca ggcgcagaac
     1621 agagcgagca taacgactgg aaagcgacgc agatcaaagt ggaagatgta ctgtttgcga
     1681 tggtgaaaga agtagaaaat cgcccagctg tttcgctgaa aaccagcccg gagctggcgg
     1741 agctgctacg tcagcagcac agcgatgtgc gtccaagccg ccatctgaat aaagcgcact
     1801 ggagcaccgt gtatctcgac ggttcgctgc cggattcgca aatctattat ctggtggatg
     1861 cgtcttatca gcaggcggtg aatttactgc cggaagaaaa acgtaaattg ctggtgcaac
     1921 tctgaaagga aaaggccgct cggaaggcgg ccttaacgat tacagcatcg gcttgaggaa
     1981 gcgtgccgta tgcgaagctt cgcactccgc gacggtttct ggcgtaccgg agacgaggat
     2041 ctcgccgccg ccactgccgc cttccggccc caggtcgaca atccagtcag cggttttaat
     2101 cacgtcgaga ttgtgctcaa tcaccacaat ggtgttgccc tgatcgcgca gtttatgcag
     2161 tacgtcgagc agttgctgaa tatcggcgaa gtgcagaccg gtggtcggct cgtcgagaat
     2221 atacagcgtc tgcccggtgc cgcgttttga cagctcacgc gccagcttca cgcgctgggc
     2281 ttcaccacca gaaagtgtgg ttgcggactg ccccaagcga atgtacgtca gaccaacgtc
     2341 catcaacgtt tgcagcttac gcgccagagc tggcaccgca tcaaagaact cacgcgcctc
     2401 ttcgatggtc atatccagca cttcgtggat ggttttgcct ttgtacttaa tctccagcgt
     2461 ttcacggtta tagcgtttac ctttgcactg gtcgcacggc acgtaaatgt ccggcaggaa
     2521 gtgcatctcc actttgatca cgccatcgcc ctgacaggcc tcgcagcgtc cgccacggac
     2581 gttaaagctg aaacgtcctg gcgtataacc acgcgcacgg gattccggta cgcccgcaaa
     2641 cagttcgcgc acaggcgtaa acacgccggt ataggtcgcc gggttagaac gcggagtacg
     2701 accaattggg ctttggtcga tatcgattac tttgtcgaaa tgctccagtc cctgaatatc
     2761 gcgatacggt gccggttcgg cgatggtcgc accattcaac tggcgttggg caatcgggaa
     2821 cagtgtgtcg ttaatcagcg tcgatttacc ggaacctgaa acccctgtga tgcaggtaaa
     2881 cagaccgact ggcagcgtta gcgtcacgtc tttcaggttg ttgccgcgtg cgccagtcag
     2941 cttaagtact ttttccggat tcgccggaac gcgtttcttc ggcacttcaa tcttgcgttt
     3001 accgctcatg tactgtccgg tcaacgactc cggcaccgcc ataatcgctt ccagcggacc
     3061 ttccgcgacc acttcaccgc catgtacacc tgcaccagga ccgatatcga tcacatgatc
     3121 agcggcgcga atcgcgtctt cgtcgtgctc caccacaatc acggtattac cgagatcgcg
     3181 cagatggata agcgtaccca acaggcgctc gttatcgcgc tggtgcaggc cgatagacgg
     3241 ctcatccagc acgtacataa cgccaaccag gcccgcacca atctggctcg ccagacggat
     3301 acgctgggct tcaccgccgg aaagcgtttc tgccgagcgg gaaagcgtca ggtaattcag
     3361 gccgacgtta acgaggaatt tcaaacgatc gccgatctct ttaagaattt tttccgcaat
     3421 cttcgcccgc tgacctgcga gtttgagatt gttgaagaat tccatcgcat gaccaatgct
     3481 catgtcggag atggcgggca gcggcgtatt ctcgacatac acgtggcgcg cttcccgacg
     3541 cagacgcgtc ccttcgcagc tggcgcacgg gcgattgctg atgaacttgg ctaattcttc
     3601 acgtaccgca ctggattctg tctctttata acggcgctcc atattgtgca gcacgccttc
     3661 gaacggatga cgacggatgg aggtatcgcc acgatcgttc atgtatttga attcaatgtt
     3721 ttctttgcca gaaccgtaca acaccacttt atgcacgttc gcgctcaggc tgccccacgg
     3781 cgcttcgacg tcgaacttat agtgatctgc cagcgatttc agcatctgga aataatagaa
     3841 gttgcggcga tcccagccac ggatcgcacc acctgccagc gacagttccg gattctggat
     3901 cacgcggtca ggatcgaaat attgctgtac gccaaggccg tcgcaggtcg ggcaggcacc
     3961 tgccgggttg ttaaacgaaa acagtcgcgg ctccagttca cgcatactgt agccgcaaat
     4021 tgggcaggcg aagttggcgg aaaacagcag ctcttccgct ttcgggtcgt ccatatccgc
     4081 cactaccgcg gtaccaccgg aaagctccag cgcggtttca aacgactcgg caagacgttg
     4141 ggtaagatcg tcacgcacct tgaagcgatc aaccaccact tcaatagtgt gtttcttttg
     4201 cagttccagt ttcggcggat cggaaagatc gcagacttcg ccatcaatac gagcacggat
     4261 gtaaccctgg cttgccaggt tctccagcgt tttggtgtgt tcgcctttgc gctctttaat
     4321 gattggcgcg agcagcatca gacgcttgcc ttccggctgc gacagcacgt tatccaccat
     4381 ctggctgacg gtttgcgccg ccagcgggac gtcgtggtcc ggacagcgcg gttcgccgac
     4441 gcgggcgtac aacaaacgca aatagtcgtg gatttcggtg attgtcccca ccgtagaacg
     4501 cgggttatga gacgtcgatt tctgctcaat tgagatggca ggagaaagcc cctcaatatg
     4561 atcgacgtcc ggcttttcca tcagtgacag aaactgccgc gcgtaggcgg aaagggattc
     4621 aacgtaacgg cgctgccctt cggcatataa ggtgtcgaaa gcgagcgagg atttgccaga
     4681 acccgaaagc ccggtcacga caatgagctt gtcgcggggg ataacgaggt tgatgttttt
     4741 gagattatgg gtgcgggcgc cccgaacttc gatcttatcc attcaccttt cccggattaa
     4801 acgctttttt gcccggtggc atggtgctac cggcgatcac aaacggttaa ttatgacaca
     4861 aattgacctg aatgaatata cagtattgga atgcattacc cggagtgttg tgtaacaatg
     4921 tctggccagg tttgtttccc ggaaccgagg tcacaacata gtaaaagcgc tattggtaat
     4981 ggtacaatcg cgcgtttaca cttattcaga acgatttttt ttcaggagac acgaacatgg
     5041 ccagcagagg cgtaaacaag gttattctcg ttggtaatct gggtcaggac ccggaagtac
     5101 gctacatgcc aaatggtggc gcagttgcca acattacgct ggctacttcc gaatcctggc
     5161 gtgataaagc gaccggcgag atgaaagagc agactgaatg gcaccgcgtt gtgctgttcg
     5221 gcaaactggc agaagtggcc agcgaatatc tgcgtaaagg ttctcaggtt tatatcgaag
     5281 gtcagctgcg tacccgtaaa tggaccgatc aatccggtca ggatcgctac accacagaag
     5341 tcgtggtgaa cgttggcggc accatgcaga tgctgggtgg tcgtcagggt ggtggcgctc
     5401 ctgcaggtgg caatatcggt ggtggtcagc cgcagggtgg ttggggtcag cctcagcagc
     5461 cgcagggtgg caatcagttc agcggcggcg cgcagtctcg cccgcagcag tccgctccgg
     5521 cagcgccgtc taacgagccg ccgatggact ttgatgacga tattccgttc tgatttgtca
     5581 ttaaaacaat aggttatatt gttttaaggt ggatgattaa agcatctgcc agccataaaa
     5641 aagaagcctc cgttatggag gcttctacgt atcaggtcaa aatcattggc cactgtggcg
     5701 gtgtctgatt catctcttct atcatcaccg actcgatgtt gtgccagatg gcggtgatgt
     5761 ccgccattgt gatgccaagc aaccccattg cgaaccagca ggcggaaacc acgcccagac
     5821 cgctgctgat cactgcaagc ccgacataat cagaccgacg aaaacggatg ttgagcgtgc
     5881 tggccagaaa catcattacg gcactaagaa gttgccagcg aagaagaacc acgccagtgg
     5941 tgagggtagc catcaaacaa ttcctctgaa aagagccgat gccctggaca gcgcggcgtt
     6001 gttcacggga ggcgtggtac actctggcta tcgcggggct tgcagaacac aaaaatgaaa
     6061 cactctgttt gtttcattaa ttttgtgaac tatatcacaa ttgattgttt gttagccaga
     6121 ttaggtcgtg acttttattg ctgtacagat tatgtggttt ttcagtggca ttaag
//
LOCUS       DBFIFI010000235         6162 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.235, whole genome shotgun sequence.
ACCESSION   DBFIFI010000235 DBFIFI010000000
VERSION     DBFIFI010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6162
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.235"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..1381
                     /locus_tag="N7B39_004260"
     CDS             <1..1381
                     /locus_tag="N7B39_004260"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HGU2424122.1"
                     /translation="GFGAFHRAHQGVYADILATEHFSDWGYYEVNLIGGEQQIADLHQ
                     QDNLYTVAEMSADAWTARVVGVVKKALHVQIDGLETVLAAMCEPQIAIVSLTITEKGY
                     FHSPATGQLMLDHPMVAADVQNPHQPKTATGVIVEALARRKAAGLPAFTVMSCDNMPE
                     NGHVMRDVVTSYAQAVDVKLAQWIEDNVTFPSTMVDRIVPAVTEDTLAKIEQLTGVRD
                     PAGVACEPFRQWVIEDNFVAGRPEWEKAGAELVSDVLPYEEMKLRMLNGSHSFLAYLG
                     YLAGYQHINDCMEDEHYRHAAYALMLQEQAPTLKVQGVDLQDYANRLIARYSNPALRH
                     RTWQIAMDGSQKLPQRMLDSVRWHLAHDSKFDLLALGVAGWMRYVGGVDEQGNPIEIS
                     DPLLPVIQKAVQSSAEGKARVQSLLAIKAIFGGDLPDNSLFTAKVTEAYLSLLAHGAK
                     ATVAKYSVK"
     gene            complement(1417..1620)
                     /gene="ydfZ"
                     /locus_tag="N7B39_004261"
     CDS             complement(1417..1620)
                     /gene="ydfZ"
                     /locus_tag="N7B39_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HGU2424123.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(1797..2483)
                     /gene="rspR"
                     /locus_tag="N7B39_004262"
                     /gene_synonym="ydfH"
     CDS             complement(1797..2483)
                     /gene="rspR"
                     /locus_tag="N7B39_004262"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HGU2424124.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(2572..3318)
                     /gene="ydfG"
                     /locus_tag="N7B39_004263"
     CDS             complement(2572..3318)
                     /gene="ydfG"
                     /locus_tag="N7B39_004263"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HGU2424125.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASIEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3455..5500
                     /gene="dcp"
                     /locus_tag="N7B39_004264"
     CDS             3455..5500
                     /gene="dcp"
                     /locus_tag="N7B39_004264"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HGU2424126.1"
                     /translation="MTTINPFLVQSTLPYLAPHFDQIANHHYRPAFDEGIQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQATLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGLRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(5545..6063)
                     /locus_tag="N7B39_004265"
     CDS             complement(5545..6063)
                     /locus_tag="N7B39_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HGU2424127.1"
                     /translation="MNINRNKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
BASE COUNT         1525 a         1484 c         1575 g         1578 t
ORIGIN      
        1 aggctttggt gcatttcacc gtgcgcatca gggcgtgtat gccgatattc ttgctacaga
       61 acatttcagt gactggggat attatgaggt caacttaatc ggcggcgaac agcaaattgc
      121 cgatttacat cagcaagata atctttatac cgttgcggaa atgtcggccg atgcgtggac
      181 ggctcgcgtc gttggcgtcg ttaaaaaagc cttgcacgta cagatagatg gcttagaaac
      241 cgtgttggct gcgatgtgtg aaccgcaaat cgcgattgtc tctctgacaa tcaccgaaaa
      301 agggtatttc cactctccgg cgaccggaca gttaatgctc gatcacccga tggtcgctgc
      361 cgacgtgcaa aatccccacc agccgaaaac agcaacaggg gtgattgttg aggcgctggc
      421 tcgccgtaaa gcggcaggac ttcccgcatt taccgtcatg tcatgtgaca acatgccaga
      481 aaacggtcat gttatgcgtg acgttgtcac ttcctacgca caagccgttg atgtaaaact
      541 agcacaatgg atcgaagata acgtgacttt cccatcaaca atggtggacc gtattgtgcc
      601 cgcagtgaca gaggatacgc tggcgaaaat cgaacagctt accggtgtgc gcgatcctgc
      661 tggcgttgcc tgtgaacctt tccgccagtg ggtaatcgaa gataactttg ttgccggacg
      721 tccggaatgg gaaaaagcgg gagccgaact ggttagtgat gtgctgcctt atgaagagat
      781 gaagttgcgc atgctcaacg gcagtcattc attcctggcg tatctgggtt atcttgccgg
      841 atatcagcac attaatgact gtatggaaga tgaacattat cgtcatgcgg cgtatgcctt
      901 gatgttgcag gaacaagcgc cgacgctgaa agtgcagggc gttgatttgc aagattacgc
      961 taaccgatta attgcacgct atagcaaccc ggcgttacgt catcgaacct ggcagattgc
     1021 gatggatggc agccagaaat tgccacagcg gatgttggat tctgttcgct ggcatctggc
     1081 gcatgacagc aagttcgatc tgctggcgct gggcgtcgcg ggttggatgc gttatgtcgg
     1141 tggtgttgat gaacagggaa atccgataga aatcagtgac ccactgttac ctgttattca
     1201 gaaggctgta caaagtagtg ccgaagggaa agcgcgcgtc cagtcattgc tggcgattaa
     1261 ggcgattttt ggtggtgatt tgccagacaa tagcttgttt actgcaaaag tgacggaagc
     1321 gtacttgtct ttattagcgc atggtgcgaa agcgaccgtg gcgaaatatt ccgtgaagta
     1381 aaaaaagcaa tgccgccatc ggcggcattc acacgcttag ttcatgccga gacgaatcag
     1441 gtccagtggt gccagtttct cttcacaacc ttcaacaact acggtttttc cgcggcggat
     1501 ttgctcagcg gtcagacctt cagtatcaat cgacaggatc ttgccagtgt gaccggtgcc
     1561 gctaaccata acacggctgc cagtggtgat tgcgttacgg ttacgatcgt aggtcgtcat
     1621 aggtgttttc tcctttctga tttacagtcg aagcccacgt aacgggcgca atataaatac
     1681 tcctgtcgag tgaaatattt tttgtttttg atcaagctca catctttttt gatgcatgca
     1741 tgagccataa ggcgggggaa aagactctca ccccggatgg gatgagagga aatgaattac
     1801 tcttcgctaa accagtcgct attttcctgg cggatctggc gcacggattc gctgatttcc
     1861 tgcaaatgtt gcgtcattgc acgttctacc gcatcgccat cacgtttttg cagggcagag
     1921 aatatatcaa gatgctggcg taacagcatt tctggtggag aaacatggtc gaaactcata
     1981 tagcgcacgc gatcaacggt cgctttcagg ttctcaatgg tatcccacgc cagttgacag
     2041 tcggcaatct gcgtcaggag ttgatggaag ttgtcatcaa gttcaaaaaa atcatccagt
     2101 tgcttgcgct caatggcaat gcgttgctgg tgaagatttt gttccagttg gtagcactgg
     2161 ctttcggtaa tcatactcgc cgcccgacgc gccaccgcgc actcaatggc ctgacggata
     2221 aaactgccgt tgcgcacctg ggccatggaa attttgttga cgtagctgcc acgctgcgga
     2281 cgaatttgaa tcaggccgtt ttccgccagt ttaataaagg cttcacgaac cggctggcgt
     2341 gacacattga aacgaacaga aacttctttt tccgacaacg gtgtgcctgg tgcaatcagg
     2401 caatggacaa tgtcgcgacg aagaatacga taaatctgct gattgacagg ctgtgtggga
     2461 ttaagttgcg tttcgacggt cattcgttct tgcttgtgag tgagttaact gcatgagtct
     2521 accacttttt tgtgacaagc aataacccgg cagttacacc gggtataaaa attactgacg
     2581 gtggacattc agcccggcat agctttgggt aaccggcatc atttccaggg tattgatatt
     2641 gacgtgagca ggcagcgttg acacccacca gacggcttcg ctgacatctt ctggcgttaa
     2701 tgcaacggta ttttgatagg ttttttccgc tttaccgtca tcgcctttaa agcggacatt
     2761 ggaaaactcg gtgccaccca ccagacccgg ttcgatgtcg gtgacgcgca ccgccgtacc
     2821 atgcaggtcc gtacgcagat tcaggctaaa ctgacgaaca aacgctttcg tcgcaccgta
     2881 aacgttacca ccggcatacg gccagctacc tgccgttgag ccaatgttaa taatatgacc
     2941 atgattacgt tcaaccatac ccggtaagac ggcgcgcgtc atatatacca ggcctttgtt
     3001 gttggtatca atcatcgttt cccagtcttc aatgctggct ttatgcgcag gctccatgcc
     3061 caacgccaga ccggcattat ttaccaggat atcaatattg caccactcgg caggaagcga
     3121 tgccagcatc tcttcaatag cggcgcggtt gcgaacgtcc agttgggcga tgtacagatt
     3181 atctcccagt tcgtctttta actcctgcaa ccgttcctgg cgacggccag tggcgataac
     3241 tttatgccct tgttgaataa aacgacgagt aatgcattca ccaaaacctg ccgttgctcc
     3301 agttactaaa acgatcatct cactgttcct caacgctttt gtgtgtcaat aacatagcac
     3361 gtgatgctta agagcgggta atactggttt gcaagattgc actccgttat cagtaagcct
     3421 acgctatgga acacctcgcg ttcaggagaa gaaaatgaca acaattaatc ctttccttgt
     3481 gcaaagcaca ctgccgtatc tggctcctca ttttgatcaa attgccaatc atcactatcg
     3541 cccggcattc gatgagggaa tacagcaaaa gcgggcagaa attgctgcca tcgcgcttaa
     3601 cccgcaaaca cctgatttca acaatactat tctggcactg gaacaaagcg gagaattact
     3661 tacccgcgtt accagcgtct tttttgcgat gactgcggcg cataccaatg atgaattaca
     3721 gcgtcttgac gagcagtttt ccgctgaact ggcggaactg gctaatgata tctatctgaa
     3781 cggtgaatta ttcgcgcggg tagatgctgt ctggcaacgc cgtgaatctc tggggcttga
     3841 tagtgaatcc atccgcctgg tggaggtgat tcatcaacgt tttgtcctag ccggagccaa
     3901 acttgcacaa gctgataaag caaaattaaa agtactgaat acagaagctg cgacactgac
     3961 cagtcagttt aaccagcggt tactggcagc aaataaatcc ggcggtctgg ttgtgaacga
     4021 tatcgcgcag ctggcaggaa tgagtgagca agagattgcg ctggcggcag aggcggctcg
     4081 cgagaaaggt ctggataaca aatggctgat tccgctgctg aataccaccc aacaaccggc
     4141 gcttgccgaa atgcgcgatc gtgcgacgcg tgaaaaactg tttattgcgg gctggacgcg
     4201 agcggaaaaa aatgatgcca atgatacccg cgctatcatt caacgtctgg tggagatccg
     4261 tgcacaacag gctacattac tcggttttcc tcattatgcc gcatggaaaa tcgccgatca
     4321 gatggcaaaa acacctgaag cagcactcaa ctttatgcgg gaaattgttc cagcggcgcg
     4381 tcaacgtgcg agcgatgaat tagcctccat acaggcggtt attgataagc agcagggcgg
     4441 gtttagcgcg cagccgtggg actgggcatt ttatgccgaa caggtccggc gagagaaatt
     4501 tgatcttgat gaggcgcagc tcaagccata ttttgaatta aacacggtgt tgaatgaagg
     4561 tgtattctgg accgcgaatc agctcttcgg tattaagttt gtcgaacgtt ttgatattcc
     4621 tgtctaccat cctgacgttc gggtgtggga aatttttgat cataatggcg tggggctggc
     4681 gttattttac ggtgatttct tcgcccgtga ttcaaaaagc ggcggtgcat ggatgggcaa
     4741 ttttgttgag caatcaacgc ttaatgaaac gcatccggta atttataacg tctgcaatta
     4801 tcagaaaccc gctgccggtg agcctgcgtt gttactctgg gatgatgtca taaccttatt
     4861 ccatgaattt ggtcatacgc tgcacggcct ttttgcccgc cagcgttatg ccacgctctc
     4921 cggcaccaac acgccgcgtg attttgtcga atttccgtcg caaatcaacg aacactgggc
     4981 aacgcatccg caggtattcg ctcgctacgc ccggcattat cagagcgggg cagcaatgcc
     5041 tgacgaactg caacagaaaa tgcgtaatgc cagcctgttc aacaaagggt atgagatgag
     5101 cgaactgctt agcgccgcac ttctcgatat gcgctggcat tgcctggaag aaaacgaagc
     5161 aatgcaggat gtcgatgatt ttgaattgcg ggcgctggtg gcagaaaata tggatcttcc
     5221 tgctataccg ccacgctatc gcagcagtta ttttgcccat atttttggtg gcggatatgc
     5281 cgcaggttat tacgcttatc tgtggacgca aatgttggcc gatgatggtt accagtggtt
     5341 tgttgagcag ggcggattaa cgcgtgaaaa tgggctgcgt tttcgcgagg cgatcctttc
     5401 cagaggtaac agcgaagatc tggaacgcct gtatcgacaa tggcgcggta aggctcctca
     5461 gattatgccg atgctgcaac atcgtggctt gaacatataa acaccaccaa tggctgtcct
     5521 ggagatatcg ccaggccagc ctggttatag cagtaactgc agcagctggg cgagggcaaa
     5581 cctcaccgct aaagctaata ccgtttcgca gtcgccagca aaatgcataa ccgcagtgta
     5641 gttctggcct ttaatatgcc acgcgaacca gacggtaccc gctggcgtac catcttcacc
     5701 gccctccggt ccgccgtagc cggtaatggc aatactgaca tcagcatccg cacgctctat
     5761 ggcaccggtt gccatttctg ctgccacttt ctcactcacc gcagaatatc gttcaagaga
     5821 ttgctggctt acgctgagga ttttcatctt tgcctgatcg gtgaaagtaa caaagcctgc
     5881 accgtaaaat ttgggtgtat cttcagctgc acacagggcg ctagccaact taccgccggt
     5941 gcatgattcg gcggtagtta agcgtaattg atccgcgata agtctttgac tcaacgcgga
     6001 tgtcaggttt tcaatagtgt cggtatctgc aagttgtaca attttatttc tattaatgtt
     6061 cactgttttt tgtccacgct tttatatgta aaggtgattt gctttttctg tatgctcgtc
     6121 caatataata attttcaaac atataaatcc gattattctg aa
//
LOCUS       DBFIFI010000236         6072 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.236, whole genome shotgun sequence.
ACCESSION   DBFIFI010000236 DBFIFI010000000
VERSION     DBFIFI010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.236"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..253
                     /locus_tag="N7B39_004266"
     CDS             <1..253
                     /locus_tag="N7B39_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HGU2424128.1"
                     /translation="SGAVIRGVFDDPENISYAGQGVRVEGSSSSLFVRTDDVRQLRRG
                     DTLTIGEENFWIDRISTDDGGSCHLWLGRGVPPAVNRRR"
     gene            265..843
                     /locus_tag="N7B39_004267"
     CDS             265..843
                     /locus_tag="N7B39_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU2424129.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGASAMAINRVASSAISQSASQV
                     ARETRVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            840..1235
                     /locus_tag="N7B39_004268"
     CDS             840..1235
                     /locus_tag="N7B39_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HGU2424130.1"
                     /translation="MKHTELRAAVLDALEKHDTGATFFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            1243..1983
                     /locus_tag="N7B39_004269"
     CDS             1243..1983
                     /locus_tag="N7B39_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU2424131.1"
                     /translation="MPTPNPLAPVKGAGTTLWVYNGNGDPYANPLSDNDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWAATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPASTSVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGEFAAVAEINVTAS"
     gene            1999..2421
                     /locus_tag="N7B39_004270"
     CDS             1999..2421
                     /locus_tag="N7B39_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HGU2424132.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPKKTQMPSMNEAVKQIEQEVLTTWPAEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            2403..2837
                     /locus_tag="N7B39_004271"
     CDS             2403..2837
                     /locus_tag="N7B39_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HGU2424133.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            2830..5379
                     /locus_tag="N7B39_004272"
     CDS             2830..5379
                     /locus_tag="N7B39_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU2424134.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSMSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGEEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQSQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRMLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAVLGDKVTYQEHLNALAQQADKFA
                     QQQRAKRAAIDAKNRGLTDRQAAREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGSWMAGLRSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGGASASGGTAIQAAAAKFHFAAGGFTGTGGK
                     YEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSMADSRSQASGTFE
                     QNNHVVINNDGTNGQIGPQALKAVYDVARKAAMDVVTGQMRDGGLFSGGGR"
     gene            5376..5705
                     /locus_tag="N7B39_004273"
     CDS             5376..5705
                     /locus_tag="N7B39_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU2424135.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNADLKTY
                     SVTLSVPRWEATALESFLAEHGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            5705..>6072
                     /locus_tag="N7B39_004274"
     CDS             5705..>6072
                     /locus_tag="N7B39_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HGU2424136.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGN"
BASE COUNT         1410 a         1486 c         1986 g         1190 t
ORIGIN      
        1 gtccggcgca gtaatacgtg gtgtttttga tgaccctgaa aatatcagct atgccggaca
       61 gggcgtgcgt gttgaaggct ccagctcgtc cctgtttgtc cggactgatg atgtgcggca
      121 actgcggcgt ggagacacgc tgaccatcgg tgaggaaaac ttctggatag accggatttc
      181 gacggatgat ggcggaagct gtcatctctg gcttgggcgg ggcgtaccgc ctgccgttaa
      241 ccgtcgtcgc tgaagggggg atgtatggcc ataaaaggtc ttgagcaggc cgttgaaaac
      301 ctcagtcgta tcagcaaaac ggcggtgcct ggtgcgtcag caatggccat taaccgcgtt
      361 gcttcatccg cgatatcgca gtcggcgtca caggttgccc gtgagacaag agtacgccgg
      421 aaactggtaa aggaaagggc caggctgaaa agggccacgg ttaaaaatcc gcaggccaga
      481 atcaaggtta accgggggga tttgcccgta atcaagctgg gtaacgcgcg ggttgtcctg
      541 tcccgacgca ggcgtcgtaa aaaggggcag cgttcatccc tgaaaggtgg cggcagcgtg
      601 cttgtggtgg gaaaccgtcg tattcccggc gcgtttattc agcaactgaa aaatggccgg
      661 tggcatgtca tgcagcgtgt ggccgggaaa aaccgttacc ccattgatgt ggtgaaaatc
      721 ccgatggcgg tgccgctgac cacggcgttt aaacagaata ttgagcggat acggcgtgaa
      781 cgtcttccga aagagctggg ctatgcgctg cagcatcaac tgagaatggt aataaagcga
      841 tgaaacatac tgaactccgt gcagccgtac tggatgcact ggagaagcat gacaccgggg
      901 cgacgttttt tgatggtcgc cccgctgttt ttgatgaggc ggattttccg gcagttgccg
      961 tttatctcac cggcgctgaa tacacgggcg aagagctgga cagcgatacc tggcaggcgg
     1021 agctgcatat cgaagttttc ctgcctgctc aggtgccgga ttcagagctg gatgcgtgga
     1081 tggagtcccg gatttatccg gtgatgagcg atatcccggc actatcagat ttgatcacca
     1141 gtatggtggc cagtggctat gactaccggc gcgacgatga tgcgggcctg tggagttcag
     1201 ccgatctgac ttatgtcatt acctatgaaa tgtgaggacg atatgccaac accaaatcct
     1261 ctggcaccgg tgaaaggggc cgggaccaca ctgtgggttt ataacgggaa cggcgaccca
     1321 tatgcaaacc cgctttcaga caatgactgg tcgcgtctgg caaaggttaa agacctgacg
     1381 cccggcgaac tgaccgctga gtcctatgac gacagttatc tcgatgatga agatgcggac
     1441 tgggccgcga ccggacaggg gcagaaatcc gctggagata ccagcttcac gctggcgtgg
     1501 atgcccggag agcaggggca gcaggcgctg ctggcgtggt ttaatgaagg tgatacccgt
     1561 gcctataaaa tccgcttccc gaacggcacg gtcgatgtgt tccgcggctg ggtcagcagt
     1621 atcggtaagg cggtgacggc gaaggaagtg atcacccgca cggtgaaagt caccaacgtg
     1681 ggacgtccgt cgatggcaga agatcgcagc acggtaacag cggcaaccgg catgaccgtg
     1741 acgcctgcca gcacctcggt ggtgaaaggg cagagcacga cgctgaccgt ggcattccag
     1801 ccggaaggcg caaccgacaa gagcttccgt gcggtgtctg cggataaaac aaaagccacc
     1861 gtgtcggtca gtggtatgac catcaccgtg aaaggtgttg ctgcaggcaa ggtcaacatt
     1921 ccggtcgtat ccggtaatgg tgagtttgct gcggttgcag aaatcaacgt caccgccagt
     1981 taatccggag aatcagcgat gttcctgaaa accgaatcat ttgaacataa cggcgtgacc
     2041 gtcacgcttt ctgaactgtc agccctgcag cgtattgagc atctcgccct gatgaaacgg
     2101 caggcagaac aggcggagtc agacagcaac cggaagttta ctgtggaaga cgccatcaga
     2161 accggcgctt ttgtggtggc gatgtccctg tggcataacc atccgaagaa gacgcagatg
     2221 ccgtccatga atgaagccgt taaacagatt gagcaggaag tgcttaccac ctggcccgcg
     2281 gaggcaattt ctcatgctga aaacgtggtg taccggctgt ccggtatgta tgagtttgtg
     2341 gtgaatgatg cccctgaaca ggcagaggac gccgggcctg cagagcctgt ttctgcggga
     2401 aagtgttcga cggtgagctg agttttgccc tgaaactggc gcgcgagatg gggcgacccg
     2461 actggcgtgc catgcttgcc gggatgtcat ccacggagta tgccgactgg caccgctttt
     2521 acagtaccca ttattttcat gatgttctgc tggatatgca cttttccggg ctgacgtaca
     2581 ccgtgctcag cctgtttttc agcgatccgg atatgcatcc gctggatttc agtctgctga
     2641 accggcgtga ggctgacgaa gagcctgaag atgatgtgct gatgcagaaa gcggcagggc
     2701 ttgctggagg cgttcgtttt ggcccggacg ggaatgaagt tatccccgct tccccggatg
     2761 tggcggacat gacggaggat gacgtaatgc tgatgacagt atcagaaggg atcgcaggag
     2821 gagtccggta tggctgaacc ggtaggcgat ctggtcgttg atttaagtct ggatgcggcc
     2881 agatttgacg agcagatggc cagagtcagg cgtcattttt ccggtacgga aagtgatgcg
     2941 aaaaaaacag cggcagtcgt tgaacagtcg atgagccggc aggcgctggc tgcacagaaa
     3001 gcgggaattt ccgtcgggca gtataaagcc gccatgcgta tgctgcctgc gcagttcact
     3061 gacgtggcca cgcagcttgc aggtgggcaa agtccgtggc tgatcctgct gcaacagggg
     3121 gggcaggtga aggactcctt cggcgggatg atccccatgt tccgggggct tgccggtgcg
     3181 atcaccctgc cgatggtggg ggccacctcg ctggcggtgg cgaccggtgc gctggcgtat
     3241 gcctggtatc agggcaactc aaccctgtcc gatttcaaca aaacgctggt cctttccggc
     3301 aatcaggccg ggctgacggc agatcgtatg ctggtcctgt ccagagccgg gcaggcggca
     3361 gggctgacgt ttaaccagac cagcgagtca ctcagcgcac tggttaaggc gggggtaagc
     3421 ggtgaggctc agattgcgtc catcagccag agtgtggcgc gtttctcctc tgcatcaggc
     3481 gtggaggtgg acaaggtcgc tgaagccttc gggaagctga ccactgaccc gacgtcgggg
     3541 ctgacagcga tggcgcgcca gttccataac gtgacggcgg agcagattgc gtatgttgct
     3601 cagttgcagc gttccggcga agaagccggg gcattgcagg cggcgaacga ggccgcgacg
     3661 aaagggtttg atgaccagac ccgccgcctg aaagagaaca tgggcacgct ggaaacctgg
     3721 gcagacagga cagcacgggc attcaaatcc atgtgggatg cggtgctgga tattggtcgt
     3781 cctgataccg cgcaggagat gctgattaag gcagaggccg cgtttaagaa agcggacgat
     3841 atctggaatc tgcgcaagga tgattatttt gttaacgatg aagcgcgggc gcgttactgg
     3901 gatgatcgtg aaaaggcccg tcttgcgctt gaagccgccc gaaagaaggc tgagcagcag
     3961 agtcaacagg acaaaaatgc gcagcaacag agcgataccg aagcgtcacg gctgaaatat
     4021 accgaagagg cgcagaaggc ttacgaacgc ctgcagacgc cgctggagaa atataccgcc
     4081 cgtcaggaag aactgaataa ggcactgaaa gacgggaaaa tcctgcaggc agattacaac
     4141 acgctgatgg cggcggcgaa aaaggactat gaagcgacgc tgaaaaagcc gaaacagtcc
     4201 ggcgtgaagg tgtctgcggg cgatcgtcag gaagacagtg ctcatgctgc cctgctgacg
     4261 cttcaggcag aactccggat gctggagaag catgccggag cgaatgagaa aatcagccag
     4321 cagcgccggg atttgtggaa ggcagaaagt cagttcgcgg tactggagga ggcggcacaa
     4381 cgtcgccagc tgtccgcaca ggagaaatcc ctgctggcgc ataaagacga gacgctggag
     4441 tacaaacgcc agctggctgt acttggcgac aaggtcacct atcaggagca cctgaacgcg
     4501 ctggcacagc aggcggataa attcgcacag caacaacggg caaaacgggc cgccattgat
     4561 gcaaaaaacc gggggctgac tgaccggcag gcagcgcggg aagccacgga acagcgcttg
     4621 aaggaacagt atggcgataa tcctctggcg ctgaataacg tcatgtcaga gcagaaaaag
     4681 acctgggcag ctgaagacca gcttcgcggg agctggatgg caggcctcag gtcaggctgg
     4741 agtgagtggg aagagagcgc cacggacagt atgtcgcagg ttaaaagtgc tgccacgcag
     4801 acctttgatg gtattgcaca gaatatggcg gcgatgctga ccggcagtga acagaactgg
     4861 cgcagcttca cccgttccgt gctgtccatg atgacagaaa ttctgcttaa gcaggcaatg
     4921 gtggggattg tcggaagtat aggcagcgcc attggcggcg gcgcatcagc gtcaggcggt
     4981 acagccattc aggcagctgc ggcgaaattc cattttgcgg ccggagggtt tacgggaacc
     5041 ggcggcaaat atgagccagc ggggattgtt caccgtggtg aatttgtctt cacgaaggag
     5101 gcaaccagcc ggattggcgt aggaaatctt taccggctga tgcgcggcta tgccaccggc
     5161 ggttatgtcg gtacaccggg cagcatggcg gacagccggt cgcaggcgtc cgggacgttt
     5221 gagcagaata accatgtggt gattaacaac gacggcacga acggtcagat agggccacag
     5281 gcactgaagg cggtttatga cgtagcccgt aaggcggcaa tggatgttgt gaccgggcag
     5341 atgcgcgatg gtggtctgtt ctccggaggt ggacgatgaa aaccttccgc tggaaagtga
     5401 aacccggtat ggatgtggct tcggcccctt ccgtaagaaa ggtgcgcttt ggtgatggct
     5461 attcccagcg agcgcctgcc gggctgaatg ccgacctgaa aacgtacagc gtgacgcttt
     5521 ctgttccccg ttgggaggcc acggcgcttg agtcgtttct ggctgagcac gggggctgga
     5581 aggcctttct gtggacgccg ccttatgagt ggcggcagat aaaggtgacc tgcgcaaaat
     5641 ggtcgtcgcg ggtcagtatg ctgcgtgttg agttcagcgc agagtttgaa caggtggtga
     5701 actgatgcag gatatccggc aggaaacact gaatgaatgc acccgtgcgg agcagtcggc
     5761 cagcgtggtg ctctgggaaa tcgatctgac agaggtcggt ggagaacgtt attttttctg
     5821 taatgagcag aacgaaaaag gtgagccggt cacctggcag gggcgacagt atcagccgta
     5881 tcccattcag gggagtggtt ttgaactgaa tggcaaaggc accagtacgc gccccacgct
     5941 gacggtttct aacctgtacg gtatggtcac cgggatggcg gaagatctgc agagtctggt
     6001 cggcggaacg gtggtccggc gtaaggttta cgcccgtttt ctggatgcgg tgaacttcgt
     6061 caacggaaac ag
//
LOCUS       DBFIFI010000237         6034 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.237, whole genome shotgun sequence.
ACCESSION   DBFIFI010000237 DBFIFI010000000
VERSION     DBFIFI010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6034
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.237"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            170..2455
                     /gene="nrdA"
                     /locus_tag="N7B39_004275"
     CDS             170..2455
                     /gene="nrdA"
                     /locus_tag="N7B39_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HGU2424137.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKHFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            2682..3812
                     /gene="nrdB"
                     /locus_tag="N7B39_004276"
     CDS             2682..3812
                     /gene="nrdB"
                     /locus_tag="N7B39_004276"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HGU2424138.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDSEVDTDDLSNFQL"
     gene            3812..4066
                     /gene="yfaE"
                     /locus_tag="N7B39_004277"
     CDS             3812..4066
                     /gene="yfaE"
                     /locus_tag="N7B39_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HGU2424139.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4120..4770)
                     /gene="inaA"
                     /locus_tag="N7B39_004278"
     CDS             complement(4120..4770)
                     /gene="inaA"
                     /locus_tag="N7B39_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HGU2424140.1"
                     /translation="MAVSAKHDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVTPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(4851..>6034)
                     /locus_tag="N7B39_004279"
     CDS             complement(4851..>6034)
                     /locus_tag="N7B39_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2424141.1"
                     /translation="KLWTKDFWAITIISFIIFFVFYVLLTLLPIYISDRLHASPDKAG
                     LLVTLFLIAAIVIRPFAGQWVGKYSNKTILVLSSLAFLVVTALYPFCHSIESLLFIRV
                     LHGITFGVITTVKGTISARLIPASRRGEGISFFSLAMGLAMVVGPWIGLNMARWEAFN
                     MAFWLCTGVAAVGIILSLIMTVPPVISHADGSKPKMGFAAMFDRAALPFAMVTFFMTF
                     SYAGVSAFLALYARELNLMSAASNFLLCYAIFLMICRTFTGNVCDKKGPKYVVYPCLL
                     FFTVGLVVLGYTQGSVMMVVSGALIGIGYGSVTPVFQTQIISSVEPHKIGVANSLFFN
                     AMDAGLALGACVMGMMVAHTGYRMIYLLGALLVVVAGGVYALQMKGKSGVALVVAKEI
                     H"
BASE COUNT         1555 a         1639 c         1492 g         1348 t
ORIGIN      
        1 tcctgagtta tccacaaagt tatgcacttg caagagggcc attttcacac tatcttgcag
       61 tgaatcccaa acataccccc tatatatagt gttctaagca gcttcccgta ctacaggtag
      121 tctgcacgaa actattgcgg aaagaattcc aaaaacaggt acgacataca tgaatcagaa
      181 tctgctggtg acaaagcgcg acggtagcac agagcgcatc aatctcgaca aaatccatcg
      241 cgttctggat tgggcggcag aaggactgca taacgtttcg atttcccagg tcgagctgcg
      301 ctcccacatt cagttttatg acggtatcaa gacctctgac atccacgaaa ccattatcaa
      361 ggctgccgca gacctgatct cccgtgatgc gccggattat cagtatctcg ccgcgcgcct
      421 ggcgatcttc cacctgcgta aaaaagccta cggccagttt gagccgcctg cgctgtacga
      481 ccacgtggtg aaaatggtcg agatgggcaa atacgataat catctgctgg aagactacac
      541 ggaagaagag ttcaagcaga tggacacgtt tatcgatcac gaccgtgata tgaccttctc
      601 ttatgctgcc gttaagcaac tggaaggcaa atatctggta cagaaccgtg tgaccggcga
      661 aatctacgaa agcgcccagt tcctttatat tctggttgct gcgtgcctgt tctcgaacta
      721 cccgcgtgaa acgcgcctgc aatatgtgaa gcatttttac gacgcggttt ccacatttaa
      781 aatttcgctg ccgacgccaa tcatgtccgg cgtgcgtacc ccgactcgtc agttcagctc
      841 ctgcgtactg atcgagtgcg gcgacagcct ggattccatc aacgccacct ccagcgcgat
      901 tgtgaaatat gtttcccagc gtgccgggat cggcatcaac gccgggcgta ttcgtgcgct
      961 gggtagcccg attcgcggtg gcgaagcgtt ccataccggc tgcattccgt tctacaaaca
     1021 tttccagact gcggtgaaat cctgctctca gggcggcgtg cgcggcggtg cggcaacgct
     1081 gttctacccg atgtggcatc tggaagtgga aagcctgctg gtgttgaaaa acaaccgtgg
     1141 tgtggaaggc aaccgcgtgc gtcatatgga ctacggggta caaatcaaca aactgatgta
     1201 tacccgcctg ctgaaaggtg aagatatcac cctgttcagc ccgtccgacg taccggggct
     1261 gtacgacgca ttcttcgccg atcaggaaga gtttgaacgc ctgtatacca aatatgagaa
     1321 agacgacagc atccgcaagc agcgtgtgaa agccgttgag ctgttctcgc tgatgatgca
     1381 ggaacgtgcg tctaccggtc gtatctatat tcagaacgtt gaccactgca atacccatag
     1441 cccgtttgat ccggccatcg cgccagtgcg tcagtctaac ctgtgcctgg agatagccct
     1501 gccgaccaaa ccgctgaacg acgtcaacga cgagaacggt gaaatcgcgc tgtgtacgct
     1561 gtctgctttc aacctgggcg caattaatag cctggatgaa ctggaagagc tggcaattct
     1621 ggcggttcgt gcacttgacg cgctgctgga ttatcaggat tacccgatcc cggccgccaa
     1681 acgtggagcg atgggtcgtc gtacgctggg tattggtgtg atcaacttcg cttactacct
     1741 ggcgaagcat ggtaaacgct actccgacgg cagcgccaac aacctgacgc ataaaacctt
     1801 cgaagcgatt cagtattacc tgctgaaagc gtctaatgag ctggcgaaag agcaaggcgc
     1861 gtgcccgtgg tttaacgaaa ccacttacgc gaaagggatc ctgccgatcg atacctataa
     1921 gaaagatctg gatgccatcg ctaatgaacc gctgcattac gactgggaag ctctgcgtga
     1981 gtcaatcaaa acacacggcc tgcgtaactc cacgctttct gctctgatgc cgtccgagac
     2041 ttcttcgcag atctctaacg ccactaacgg tattgaaccg ccgcgcggtt acgtcagcat
     2101 caaagcgtcg aaagacggta ttctgcgcca ggtggtgccg gactacgagc atctgcacga
     2161 cgcctatgag ctgctgtggg aaatgccggg taacgatggc tacctgcaac tggtgggtat
     2221 catgcagaaa tttatcgatc agtcgatctc tgctaacacc aactacgatc cgtcacgctt
     2281 cccgtcagga aaagtgccga tgcagcagtt gctgaaagac ctgctcaccg cctacaaatt
     2341 cggggtcaaa acgctgtatt atcagaacac ccgtgacggc gctgaagacg cgcaggacga
     2401 tctggtgccg tcaatccagg acgatggctg cgaaagcggc gcatgtaaga tctgatattg
     2461 agatgccgga tgcggcgtaa acgccttatc cggcctacgg ctcggtttgt aggcctgata
     2521 agacgcgcca gcgtcgcatc aggcacagga tgcggcgtga acgcctaatc cggcctacgg
     2581 ctcggatttg taggcctgat aagacgcgcc agcgtcgcat caggcacagg atgcggcgta
     2641 aaatgcctta tccggcatta aactcccaac aggacacact catggcatat accacctttt
     2701 cacagacgaa aaatgatcag ctcaaagaac cgatgttctt tggtcagccg gtcaacgtgg
     2761 cccgttacga tcagcaaaaa tatgacatct tcgaaaagct gatcgaaaag cagctctctt
     2821 tcttctggcg tccggaagaa gttgacgttt ctcgtgaccg tatcgattac caggcgctgc
     2881 ctgaacacga aaaacacatc tttatcagca acctgaaata tcagacgctg ctggactcca
     2941 ttcagggtcg tagcccgaac gtggcactgc tgccgctgat ctcgatcccg gaactggaaa
     3001 cctgggtaga aacctgggcg ttctcagaaa cgattcattc ccgttcctat actcatatca
     3061 ttcgtaatat cgttaacgat ccgtctgttg tgtttgacga tatcgtcacc aacgagcaga
     3121 tccagaaacg tgcggaaggg atctccagct actacgatga gctgatcgaa atgaccagct
     3181 actggcacct gctgggcgaa ggcacccaca ctgttaacgg taaaaccgtg accgttagcc
     3241 tgcgcgagct gaagaaaaaa ctgtatctct gcctgatgag cgttaacgcg ctggaagcga
     3301 tccgcttcta cgtcagcttt gcttgttcct ttgcctttgc agaacgcgaa ctgatggaag
     3361 gtaacgccaa aattattcgc ctgattgccc gcgacgaagc cctgcacctg accggtactc
     3421 agcatatgct gaatctgctg cgcagcggtg cggatgatcc ggagatggcg gaaattgccg
     3481 aagaatgtaa gcaggagtgc tatgacctgt ttgtccaggc ggcacagcaa gagaaagact
     3541 gggcggatta tctgttccgt gacggttcga tgattggtct gaataaagac attctctgcc
     3601 agtacgttga atacatcacc aatatccgca tgcaggcagt cggtttggat ctgccgttcc
     3661 agacgcgctc caacccgatc ccgtggatca acacctggct ggtgtctgat aacgtgcagg
     3721 ttgctccgca ggaagtggaa gtcagttctt atctggtcgg gcagattgac tcggaagtgg
     3781 acaccgacga tttgagtaac ttccagctct gatggctcgc gttaccctgc gcatcactgg
     3841 cacacaactg ctgtgccagg atgaacaccc ttcccttctg gcggcgctgg aatcccacaa
     3901 tgtggcggtt gagtaccagt gccgcgaagg ttactgcggc tcctgtcgca cacgcctggt
     3961 tgcaggccag gtggactgga ttgccgaacc gttagccttt attcagccgg gggaaatttt
     4021 gccctgttgt tgccgagcaa aaggcgatat tgaaatcgag ttgtgatcgg gtactagcgc
     4081 cagatacgac gcttacgcgt attatctggc ctacaacgat tacatggcgt agtaatacgc
     4141 ttttacctgc tcccagtccg ctttggggat cggctccaga tatttttcca gctggcggaa
     4201 gtcatgatta atcgctttat cgcgacgcaa gcggcgacga cttttctcca gatcaagaaa
     4261 accagcttct gccttacctt ctgttttcac atagatatgg cgaacataac aacagccatg
     4321 ctgacgatta acgctatgca ttttcttaaa cgccagcgcc actgctttca acatggcttg
     4381 ccgtacttcg tcagaatagg gcgttactgc atgctgggca taccagtcag caatgctgat
     4441 gaaccccgcc atatcttcag tcaccagcaa cgcgcgccat tccccctcaa ttttcaccgc
     4501 ttcaccaaaa acgatcttcg gcacgatgac accggcccgt tccagttctt taattaccgc
     4561 aacctcacgg acaatcgttg gtcggccgaa aggataacgt acggaatgaa acagatgatg
     4621 cgtcatgcgc tttacatata gctttttgcc gttgcgctcg acgcattgca ccccgctcat
     4681 accattacgg cgatagttag gctcttcaac ccagtcgccc tctgttgccc accagtgatt
     4741 aaattcgtcg tgctttgctg aaactgccat accctatcgc ctgtcatttt ttattaacga
     4801 caatgactat aggtggttac ctgcgaaaaa tcttaatgaa acgtgtcgta ttaatgaatt
     4861 tcttttgcca ctactagcgc gacaccgctt tttcccttca tttgcagcgc atagactcca
     4921 ccagccacta ccactaatag tgcgcccagc agataaatca ttcggtagcc agtatgtgca
     4981 accatcatcc ccatcacaca ggctcccagc gccaggcctg catccatcgc attgaagaag
     5041 agggagtttg cgacaccgat tttatgcggt tccactgaac tgataatctg cgtctgaaaa
     5101 actggcgtca cggaaccata cccgatacca atcaacgcgc cagaaacgac catcattacg
     5161 ctgccctggg tgtagccgag aaccaccagc ccaaccgtaa agaacagcag gcaggggtaa
     5221 accacatatt tcgggccttt tttgtcgcaa acattgccgg tgaaggtacg gcagatcatc
     5281 aggaagatgg cgtagcagag caggaaatta ctggccgccg acatcagatt aagttcgcgg
     5341 gcgtaaagcg ccagaaaggc agaaaccccg gcatacgaaa aggtcataaa gaatgtaacc
     5401 atggcaaacg gcaatgccgc gcgatcgaac atggcggcga agcccatctt tggctttgaa
     5461 ccgtcggcat ggctgataac cggcggcacg gtcataatca gcgacaggat aatccccacc
     5521 gccgcaacgc cagtacataa ccagaaagcc atattaaagg cctcccagcg cgccatattc
     5581 aggccaatcc acggcccgac caccattgcc agccccattg ccagagagaa aaaactgatg
     5641 ccctccccac gtcgggaggc cgggatcagc cgcgcggaaa tcgttccctt taccgttgtg
     5701 ataaccccga aggtaatacc atgaagcacc ctaataaaaa gcagtgattc tattgagtgg
     5761 caaaaaggat acagcgcagt gaccaccaaa aaggccagag aagagagcac cagaatagtt
     5821 ttattcgaat atttacccac ccattgcccg gcaaagggtc gaataacaat cgccgcaatt
     5881 aaaaataaag tcaccaacaa acctgcttta tcaggagagg catgcaagcg gtcagagata
     5941 taaattggca acaatgttag taaaacataa aagacgaaga aaataataaa gctgatgatg
     6001 gttattgccc aaaaatcctt cgtccataac ttct
//
LOCUS       DBFIFI010000238         6033 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.238, whole genome shotgun sequence.
ACCESSION   DBFIFI010000238 DBFIFI010000000
VERSION     DBFIFI010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6033
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.238"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..200
                     /locus_tag="N7B39_004280"
     CDS             <1..200
                     /locus_tag="N7B39_004280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424142.1"
                     /translation="PCLNESDEHKGNQYSRWISVVLGELLIKIWNCDTVQIQTHKKTS
                     SLALERFLADLITINYEAQKK"
     gene            235..2940
                     /gene="malT"
                     /locus_tag="N7B39_004281"
     CDS             235..2940
                     /gene="malT"
                     /locus_tag="N7B39_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HGU2424143.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            3007..3285
                     /locus_tag="N7B39_004282"
     CDS             3007..3285
                     /locus_tag="N7B39_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HGU2424144.1"
                     /translation="MKPESKEAPINIRAKASQRDLIDMAANLVAKSRTDFMLDAACRE
                     AQDILLDQRLFILDDEQYDAFLAALDAPITAERQAKINALMNRKSPWE"
     gene            3276..3761
                     /locus_tag="N7B39_004283"
     CDS             3276..3761
                     /locus_tag="N7B39_004283"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU2424145.1"
                     /translation="MGITAPTPLTSEHNLADFCCSDHGMNEWLKKKALKNHSSGLSRV
                     YVICIANTRQVIGYYCLSTGSIQRNLAPGAMRRNAPESLPVVVLGRLAIDQAWAGKGL
                     GVALLKDAVYRTMSIAQQVGVRALIVHALDDSVRNFYLKYAFVPSPFQSLTLLYPITL
                     E"
     gene            complement(3811..4839)
                     /gene="rtcA"
                     /locus_tag="N7B39_004284"
     CDS             complement(3811..4839)
                     /gene="rtcA"
                     /locus_tag="N7B39_004284"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HGU2424146.1"
                     /translation="MKRMIALDGAQGEGGGQIMRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAATEICGATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAASFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGQFTVAHPSCHLLTNIAVVERFLPVRFTLAETDGVTR
                     VMITKLTD"
     gene            complement(4843..>6033)
                     /gene="rtcB"
                     /locus_tag="N7B39_004285"
     CDS             complement(4843..>6033)
                     /gene="rtcB"
                     /locus_tag="N7B39_004285"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HGU2424147.1"
                     /translation="VKMWTKGVPVEADARQQLINTAKMPFIFKHIAVMPDVHLGKGST
                     IGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQAIETAVPHGRTTG
                     RCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHLGTLGTGNHFIEI
                     CLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRDLAYFMEGTEYFD
                     DYLKAVAWAQLFASLNRDVMMENVVTALQSITQKTVRQPQTLAMEEINCHHNYVQKEQ
                     HFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCSCSHGAGRVMSRT
                     KAKKMFSVEDQIRATVHVECRKDAEVIDEIPMAYKDIDAVMAAQSDLVEVIYTLRQVV
                     CVKG"
BASE COUNT         1453 a         1681 c         1493 g         1406 t
ORIGIN      
        1 acccttgctt aaatgaatct gatgaacata agggaaacca gtattcacgc tggatcagcg
       61 tcgttttagg tgagttgtta ataaagattt ggaattgtga cacagtgcaa attcagacac
      121 ataaaaaaac gtcatcgctt gcattagaaa ggtttctggc cgaccttata accattaatt
      181 acgaagcgca aaaaaaataa tatttcctca ttttccacag tgaagtgatt aactatgctg
      241 attccgtcaa aattaagtcg tccggttcga ctcgaccata ccgtggttcg tgagcgcctg
      301 ctggctaaac tttccggcgc gaacaacttc cggctggcgc tgatcacaag tcctgcgggc
      361 tacggtaaga ccacgctcat ttcccagtgg gcggcaggca aaaacgatat cggctggtac
      421 tcgctggacg aaggtgataa ccagcaagag cgtttcgcca gctatctcat tgccgccgtg
      481 caacaggcaa ccaacggtca ctgtgcgata tgtgagacga tggcgcaaaa acggcaatat
      541 gccagcctga cgtcactctt cgcccagctt ttcattgagc tggcggaatg gcatagccca
      601 ctttatctgg tcatcgatga ctatcatctg atcactaatc cagtgatcca cgagtcaatg
      661 cgcttcttta ttcgccatca accagaaaat ctcaccctgg tggtgttgtc acgtaacctt
      721 ccgcaactgg gcattgccaa tctgcgtgtt cgtgatcaac tgctggaaat tggcagtcag
      781 caactggcat ttacccatca ggaagcgaag cagttttttg attgccgtct gtcatcgccg
      841 attgaagccg cagaaagcag tcggatttgc gatgacgttt ccggttgggc gacggcacta
      901 cagctaatcg ccctctccgc ccggcagaat acccactcag cccataagtc ggcacgccgc
      961 ctggcgggaa tcaatgccag ccatctttcg gattatctgg tcgatgaggt tttggataac
     1021 gtcgatctcg caacgcgcca ttttctgttg aaaagcgcca ttttgcgctc aatgaacgat
     1081 gcactcatca cccgtgtgac cggcgaagaa aacgggcaaa tgcgcctcga agagattgag
     1141 cgtcaggggc tgtttttaca gcggatggat gataccggcg agtggttctg ctatcacccg
     1201 ctgtttggta acttcctgcg ccagcgctgc cagtgggaac tggcggcgga gctgccggaa
     1261 atccaccgtg ccgccgcaga aagctggatg gcccagggat tccccagcga agcgattcat
     1321 catgcgctgg cggcaggcga tgcgctgatg ctgcgcgata ttctgcttaa tcacgcctgg
     1381 agtttgttca accatagcga actgtcgctg ctggaagagt cgcttaaggc cctgccgtgg
     1441 gacagtttgc tggaaaatcc gcagttggtg ttattgcagg cgtggctgat gcaaagccaa
     1501 catcgctacg gcgaagttaa caccctgctg gcccgtgctg aacatgaaat caaggacatc
     1561 agagaaggca ccatgcacgc agaatttaac gctctgcgcg cccaggtggc gattaacgat
     1621 ggtaatccgg atgaagcgga acggctggca aaactggcac tggaagagct accgccgggc
     1681 tggttctata gccgcattgt ggcaacctcg gtgctgggtg aagtgctgca ctgcaaaggc
     1741 gaattgaccc gctcactggc gctaatgcag caaaccgaac agatggcacg ccagcacgac
     1801 gtctggcact acgccttatg gagtttaatt cagcaaagtg aaattctgtt tgcccaaggg
     1861 ttcctgcaaa ccgcgtggga aacgcaggaa aaagcgttcc agctgatcaa cgagcagcat
     1921 ctggaacagt tgccgatgca tgagtttctg gtgcgcatcc gagcacaact gttatgggcc
     1981 tgggcacggc tggatgaagc cgaagcgtcg gcgcgtagcg ggattgaagt cttatcgtct
     2041 tatcagccac agcaacagct tcagtgcctg gcaatgttga ttcaatgctc gctggcccgt
     2101 ggtgatttag ataacgcccg tagccagctg aaccgtctgg aaaacctgct ggggaatggc
     2161 aaatatcaca gcgactggat ctctaacgcc aacaaagtcc gggtgattta ctggcaaatg
     2221 accggcgata aagccgccgc tgccaactgg ttgcgtcata cggctaaacc ggagtttgcg
     2281 aacaaccact tcctgcaagg tcaatggcgc aacattgccc gcgcacaaat cttgctgggc
     2341 gagtttgaac ctgcggaaat tgtcctcgaa gaactcaatg aaaatgcccg cagtctgcgg
     2401 ttgatgagcg atctcaaccg taacctgttg ctgctaaatc aactgtactg gcaggccgga
     2461 cgtaaaagtg acgcccagcg cgtgttgctg gacgcattaa aactggcgaa tcgcaccgga
     2521 tttatcagtc attttgtcat cgaaggcgaa gcgatggcgc aacaactgcg ccagctgatt
     2581 caactcaata cgctgccgga actggaacag catcgcgcac agcgtattct acgagaaatc
     2641 aatcaacatc atcggcataa attcgcccat ttcgatgaga atttcgttga acgtctgcta
     2701 aatcatccgg aagtgccaga actgatccgc accagcccgt tgacccaacg tgaatggcag
     2761 gtcctggggc tgatttactc cggttacagc aacgaacaaa tagcgggtga actggaagtg
     2821 gcggcaacca ccatcaagac gcatatccgc aatctgtacc aaaaactcgg cgtcgcccac
     2881 cgtcaggctg ccgtgcagca cgcgcagaaa ctgctgaaga tgatgggtta tggggtgtga
     2941 gtttgtatag cgtttttatg tacactcttt atactgtata taaaatcgca ctacaggctc
     3001 atgaatatga aaccggaaag caaagaagcg ccaatcaata tcagagcgaa agcctcacag
     3061 cgtgacctga ttgatatggc agcgaatctg gtcgcaaaat cgcgcactga ttttatgctg
     3121 gatgccgcat gccgtgaagc gcaagacatc ttattagatc aacgactatt catccttgat
     3181 gacgagcaat acgatgcgtt tctcgctgcc ctggacgccc ccatcactgc tgagcgtcag
     3241 gccaaaatta acgcgttgat gaatcgtaaa tcgccatggg aataacggct cctactcccc
     3301 taacgtctga acataatctg gctgactttt gctgttccga tcacgggatg aatgaatggc
     3361 taaaaaagaa agcgttaaaa aatcacagct ccggtctgtc tcgtgtttac gttatctgta
     3421 ttgccaatac ccgccaggta atcggctatt actgcctttc tacaggcagt attcaacgta
     3481 atttggcccc tggagccatg cgacgtaatg ctcccgaatc gttgcctgtt gtcgtattag
     3541 gcagactggc aattgatcaa gcctgggcag gtaaagggtt gggcgtcgca ttgttgaaag
     3601 atgcagtcta tcgcacaatg tccatagctc aacaggtagg ggttcgtgcg ctcattgttc
     3661 atgccttaga cgattcagta cgcaatttct atctgaagta tgcctttgta ccttcaccat
     3721 tccagtcact taccttactg tatcccatta ctctggagta atttacctct accggatagt
     3781 cacactgatg agtctgatcc gggagggatt tcaatcagtg agtttggtta tcatcacccg
     3841 cgttacgcca tctgtttcag ccagagtaaa ccgcaccggc aagaaacgct ccaccacggc
     3901 gatattggtc agcagatggc atgagggatg ggcgaccgta aattgtcccg cgcccgccag
     3961 cgccattggc aacactagct ggtcagcaag atattccccc actgccgccg ggcttgccag
     4021 gtagcgtttc acctctttca ccaactgtgc cgcaaccacc tcggcactga cgcgcttttc
     4081 accgacgaca aaaaagcgtt cggtgatatt ttcactttcg acttcaagcg agacggtatt
     4141 acccggcccc tggtcgcgcg gcaggttatg aatattctgc tcatgcaggg aaaaacttgc
     4201 cgccagcgta gcgatttcac gctcagcgac atgccgcggc acgccagcta ataacacctc
     4261 tccacgcatc tgcacaatgt tcccgcgctc gccaagttgc aaggtgttaa acgatgccac
     4321 cggcgagact tccgttgcca ccacgccgcc tccggcagga taaaaaccgt gacgtaacag
     4381 cgtggtttgc tgatgaattc ctattttcgc cagcagcggc tccaacaccc ggcggataaa
     4441 atccgcaggc ggggccgacg ggttatcggt accgccgctc acttcaacac gcgaaggtcc
     4501 atcggcaaac cacagcgcgg gcagcaccgt ttgcagcacc agcgtacaac ttcctgcgct
     4561 accgatagca aagcgataat cgccgccacg cacggtgccg ggccggaaga ccagacgctg
     4621 cgaccccagc tccgcgcctt ccaccgtcgc cccgcaaatt tccgtcgccg cctttaccgc
     4681 cgtcagatgc tggcgcaaca gtcccggttt tgcccgcccg gcacgaatgc cggtgatggt
     4741 aaatggctgg ccggttatca tcgacagact cagcgccgag cgcatgatct gcccgccgcc
     4801 ttcgccctgt gcgccatcca gcgcaatcat ccttttcatc atttatcctt ttacgcacac
     4861 cacctgacgt agggtgtaga taacttccac cagatcgctt tgtgccgcca tcaccgcatc
     4921 aatatcttta tacgccatcg ggatttcgtc gatcacttcg gcatctttac ggcattccac
     4981 atgcacggtg gcacgaattt gatcttccac gctgaacatt tttttcgctt tagtacggct
     5041 cataacccgc ccggcaccgt ggctgcacga acagaacgac tcttcatttc ccagcccacg
     5101 gacgataaag cttttcgctc ccatcgaccc tggaataatt ccatattgac cagcacgcgc
     5161 agacaccgcg cctttacgcg tcacgtagat ctcttcacca aagtgctgtt ctttttgcac
     5221 atagttgtgg tgacagttga tctcttccat cgccagcgtt tgtggctgtc tgaccgtttt
     5281 ctgcgtaatg ctctgcaacg ccgttactac gttttccatc atcacatcgc ggttaaggct
     5341 ggcaaaaagc tgcgcccagg ccacggcttt caggtaatca tcaaagtatt ccgtaccttc
     5401 cataaagtac gccagatcac gcgacggcaa tgtttcaagc gtttcctgca tctctttttg
     5461 cgccagatcg ataaagtaag tcccgatggc attaccaatt ccgcgtgaac cggagtgcag
     5521 cataatccac acctggtccg actcatcaag gcagatttca ataaagtggt tacccgttcc
     5581 cagcgttccc aggtgtttat agttgttagt gttaaggaaa cgcggatatt tttgcgttaa
     5641 ccactgataa ccggcctcca gctcagccca tttcgcatcg acgttaacag gcgggttttc
     5701 ccatgcgcct ttatcacgtt tacaacggcc agtggtacgc ccgtgcggca ccgcagtttc
     5761 aatcgcctga cgcagctccg ccaggttttc cggcaggtca gccgccgtta aagcggtacg
     5821 tagtgcgttc attccacagc caatatccac gcccaccgcc gccggaataa tcgccccttt
     5881 ggtcgggatc acgctaccaa tggtggaacc tttacccagg tgtacatcag gcattaccgc
     5941 aatatgtttg aaaataaacg gcatcttcgc cgtattaata agttgctgac gcgcatcggc
     6001 ttctaccggc acgcctttgg tccacatttt tac
//
LOCUS       DBFIFI010000239         6032 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.239, whole genome shotgun sequence.
ACCESSION   DBFIFI010000239 DBFIFI010000000
VERSION     DBFIFI010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6032
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.239"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..203)
                     /locus_tag="N7B39_004286"
     CDS             complement(<1..203)
                     /locus_tag="N7B39_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HGU2424148.1"
                     /translation="MAVKISGVLKDGTGKPVENCTIQLKARRTSSTVVVNTVASENPD
                     EAGRYSMDVEYGQYSVILLVEGFP"
     gene            complement(268..867)
                     /locus_tag="N7B39_004287"
     CDS             complement(268..867)
                     /locus_tag="N7B39_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HGU2424149.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEQQATLSAGYLHARTSAPG
                     SDNLNGINVKYRYEFTDTLGMVTSFSYAGDRNRQLTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYKF"
     gene            complement(935..1657)
                     /locus_tag="N7B39_004288"
                     /pseudo
     CDS             complement(935..1657)
                     /locus_tag="N7B39_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
     gene            1645..2061
                     /locus_tag="N7B39_004289"
                     /pseudo
     CDS             1645..2061
                     /locus_tag="N7B39_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2303 family protein"
     gene            2189..2725
                     /gene="yfdR"
                     /locus_tag="N7B39_004290"
     CDS             2189..2725
                     /gene="yfdR"
                     /locus_tag="N7B39_004290"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HGU2424150.1"
                     /translation="MSFIKTFSGKHFYYDRINKDDIDINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFNVIPLAPSHA
                     YGMFMERFNELSELHKCA"
     gene            2716..3066
                     /locus_tag="N7B39_004291"
     CDS             2716..3066
                     /locus_tag="N7B39_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001242737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eae protein"
                     /protein_id="HGU2424151.1"
                     /translation="MRMNVFEMEGFLRGKCVPRDLKVNETNAEYLVRKFDEVRAEARN
                     EGINYTASRLAAAFNHGFINKPLAEVFDVTRMILSAKEELANESHPIDGLSGEYAEKS
                     LEEWAERLRKGGSQ"
     gene            3063..3536
                     /locus_tag="N7B39_004292"
                     /pseudo
     CDS             3063..3536
                     /locus_tag="N7B39_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145689.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
     gene            3683..3880
                     /locus_tag="N7B39_004293"
     CDS             3683..3880
                     /locus_tag="N7B39_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424152.1"
                     /translation="MAELTKWLQNTITGIETVVDDKSFVCDEIVFKIDVVKNVLTAFK
                     VALVSLEVLMILCRSAMLSQN"
     gene            3838..4188
                     /locus_tag="N7B39_004294"
                     /pseudo
     CDS             3838..4188
                     /locus_tag="N7B39_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
     gene            4225..4506
                     /locus_tag="N7B39_004295"
     CDS             4225..4506
                     /locus_tag="N7B39_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyocin activator PrtN family protein"
                     /protein_id="HGU2424153.1"
                     /translation="MNTLFLLMAEFNTPNIELSAVSQKYFGMSPATAEAKANACKLPV
                     PTYRIGTSQKAKRCINIQDLAEYIDKRREEGRIEWEQVRTVKQKDKEHH"
     gene            complement(4554..4727)
                     /locus_tag="N7B39_004296"
     CDS             complement(4554..4727)
                     /locus_tag="N7B39_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424154.1"
                     /translation="MKDQKATKPQVKFDTMKAFAGMGAAVEVLMKAAPNAFTHATVSG
                     KEQQGKLRRRKAA"
     gene            complement(4924..5457)
                     /locus_tag="N7B39_004297"
     CDS             complement(4924..5457)
                     /locus_tag="N7B39_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424155.1"
                     /translation="MLTLDEIGQSVRNNIQLIIDHVGLPLAVGPLSDDDYKILCGGYG
                     ELEWDYALSTYGNSREKYEFCIKLVQQGRVQGIPSGAAICVYGVEENIFRIHMIERFS
                     REDESHPLKGRMVLLTLMSAFIFCKAVECKVVHIVEPVPELVQYYESFGFRMEQCGYV
                     MSAVIDELQDIFLKFAQ"
     gene            complement(5604..5708)
                     /locus_tag="N7B39_004298"
                     /pseudo
     CDS             complement(5604..5708)
                     /locus_tag="N7B39_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
BASE COUNT         1679 a         1446 c         1363 g         1544 t
ORIGIN      
        1 gggaatcctt ccaccaacag aatgacgctg tactgaccgt actcaacgtc catgctgtaa
       61 cgaccggctt catccggatt ttcagaggcc accgtgttca ccaccaccgt gctgctggtc
      121 cgtctggctt tcagttgaat ggtgcagttc tctaccggtt ttcctgtgcc gtctttcagt
      181 acacctgaaa tctttactgc catattcacc ccacaaaaaa gcccgcctga accggcgggc
      241 tgtcataaca ctgtgttacc tggctaatca gaatttatag ccgacaccca cgatgaagcc
      301 gtcagtgcgc cagtcgccac tgccggagcc ttcataagca atatcaatgg ccacggattc
      361 ggtcgggtta aactgcacgc cagctcccca cgccagagac gtgttgctgt ggcgaccgtc
      421 atcacttccg gtcagcacat catgcgtttt ccccttgttg tcagttacgc ggagataatc
      481 cccggagaaa gtcgacacac ggctgtaagc cacacccgcc atcgcatacg cgctgaacca
      541 ttcattcacg cgcacagagg gccccgccat cacgctgaac cagcggttac gcacggaatc
      601 ttcatgccag cgggtatcgc tgtaacgggt aagctggcga ttcctgtctc ctgcatagct
      661 gaatgacgtc accatcccca gtgtgtccgt aaactcataa cggtatttca cgttaatccc
      721 gttaagatta tcgctaccgg gagcgctcgt ccgggcatga agataccccg cgctcagcgt
      781 ggcctgctgc tcagacgccc atgcaggcgc gccggatacg gccagacaga tggctgcgga
      841 caaaatggcg gcataaagtt tacgcataat tacctctcgc ttttctgcaa taaaaaaggc
      901 gccatttctg gcgcccgtat atgggttata aaattcagct gatactgatg cctgcggtgg
      961 ctttcttcat caccacaacc agcaaatcgc tgatacttgc tgtgggatac cagtcattta
     1021 ccagccatgc tgacaccgaa aactccagcg tcatgtgacc gtgaccggca ggcatatcaa
     1081 taacgccact gtaaatcagc gtattatcca gcgcggtacg gttataaatt tcagcaccgt
     1141 ttttccgcac tatcagacgg catgaggagt aaatatcagt atgctctctc tcatgcttag
     1201 cgccactgaa tgccaccgcc ggaataacaa tctgccggtc aaacggctga tcgtcataaa
     1261 ccctgacggt aatggtccct gatggccacc gttccggtgc acgggagtcc cgggggaaag
     1321 ctttgcccac tgttttaacg agatcgcctt caatctggtt cgcggacagt tttcccagaa
     1381 cccgacagtt ctcgttaatc gtgacgttgt tgagcgtccc ggagttcgca ttcacgttac
     1441 cactgatatc cgcattttta gcagtcagct ttccatccgg tgtcagggaa aatgccggtg
     1501 gatttccacc actggtaatg gtgggggccg tcaggcgctt caggaacacg tcgttcatga
     1561 atatctggtt gccctgcgcc acaaacatcg gcgtttcatt cccgtttgcc gggtcaataa
     1621 acgcgatacg attggcggca accagaatgg attgaagact gggccgacta ccttgtgggc
     1681 tttgatgcta atggtgacac cattcaggca acaaaagcgg ctgcggcggt ccgtaaaatc
     1741 acgattgaag caaaccagac cgctgatttt gaagataatg acttcagcgg caaacgctcc
     1801 ctgatggagt ctgtcgaagc gaagaccaaa gacattatgc cagtggcatt tgaatttaaa
     1861 tgcattccgt ttgaaggtct gaaagaacgt ccgtttaaat tacgcctcag cattatcact
     1921 ggcgatcgtc cggtactggt tctgcgcatt attcagctgg aggcggtgca ggaagaaatg
     1981 gctaacgaat ttcgtgatct gcttgttgag aaattcaagg acagcaaagt agaaaccttt
     2041 attggtactt tcaccgcctg atttcattac tgcaaatgcc cctgaggggg catttatgga
     2101 aacgtaattt actcaataat cgccggatgg tgagggattc tttttaccag aattcagcgc
     2161 ggtgcagcgc atatacgtgg agaacaaaat gtcatttatt aaaacttttt ccgggaagca
     2221 tttttattat gacaggataa ataaagacga catcgatatt aacgatatcg cggtttccct
     2281 ttcaaatatc tgtcgctttg ccggtcatct ttcgcacttc tacagcgtcg cccaacatgc
     2341 ggttctttgc agccagctgg taccgcagga atttgctttt gaagcattaa tgcatgatgc
     2401 aacagaagcg tattgccagg acatccccgc tccactgaaa cgccttcttc ctgactataa
     2461 acggatggaa gaaaaaattg acgccgtaat ccgtgagaaa tacgggttac ccccagttat
     2521 gagtacgccc gtgaaatatg ccgatcttat catgctggca accgaacgcc gcgatctcgg
     2581 gcttgatgat ggctctttct ggcctgtact ggaaggcatc ccggcaacag agatgttcaa
     2641 cgtgattcca ctggcaccta gccatgccta cgggatgttt atggaacgtt ttaacgagtt
     2701 atcggagtta cacaaatgcg catgaatgtt ttcgaaatgg aagggtttct tcgcgggaaa
     2761 tgtgtaccgc gagatctgaa agtgaatgaa acaaatgctg agtatctggt gcgtaaattc
     2821 gatgaagtac gtgctgaggc tcgcaacgag ggtattaact ataccgcaag ccgtcttgct
     2881 gctgctttca atcacggatt tatcaataag cctttggctg aagttttcga cgttacacgc
     2941 atgattctgt cagcaaaaga agagttagct aatgaatcgc atccgattga tggcctgtcc
     3001 ggtgaatatg cagagaaatc ccttgaagaa tgggcggaac ggcttcgcaa aggaggcagc
     3061 cagtgactgg acatgcagca atcctcgaca tgtgctgtgg cagtcgcatg ttctggttcg
     3121 ataagaatga cgaccgggcg atatttagcg atatcagaaa ggaagagcac acattgtgtg
     3181 atggacgacg cctgattatc agtcctgatc tgatagctga ttttcgtgca ctaccatttg
     3241 cagacgcatc tttttcgatg gttatattcg accctccgca tcttgagcgt gttggtgata
     3301 acgcctggat gggaaagaaa tatggacggc tgaataaaga tacctagcgt gatgatttgc
     3361 ggcagggatt taaagaagcc tttcgtgtgt tgcgtccatc cggcgttctg atttttaaat
     3421 ggaatgaaac gcaaatacct gttcgccaga tattggtact gaccgacaga aaacctgtta
     3481 tcggtcaacg aacaggaaaa aacgataaaa cccactggat tatttttatg aaataggcat
     3541 ccagtgagta ggttcgtaag gttacagata cgtatatctg aataattaaa ttcagttctg
     3601 taaataaaat ttaatcctta accggaggga ttcctgcacc ctcagaacat caggagaccg
     3661 cccgaaaggg cggtaatgaa aaatggctga attaaccaaa tggctacaaa acacgattac
     3721 cggaattgaa acggtagtag acgataaatc gtttgtatgt gatgaaatag tattcaaaat
     3781 cgatgtggtt aaaaacgtac ttaccgcatt taaagtcgcg ctggtatcgc tggaagtatt
     3841 gatgatactt tgtcgatcag cgatgctctc tcagaactaa accatttcat taaccggcac
     3901 gcagacaata cgaaatattt aaaagtctgg ggtaacgggg ccaccttcga caacgtaatt
     3961 ttacgtggag cttacgagcg agcaggacaa atctgcccgt gggcgtactg gaatgaccac
     4021 gatgtacgca cgatcgttac gcttgggcgt tccatcggat tcgaccccaa aatggacatg
     4081 cctttcgatg gcgaacggca caacgccctg gctgatgccc gtcatcaggc aaaatatgtt
     4141 tccgctatct ggcagaaatt aattcctgcc accagcacag aattatgatt ttcccgggtg
     4201 cagccggttt tgatggagaa aattatgaac accttgtttt tactgatggc tgaattcaat
     4261 accccaaaca ttgaactctc agcagttagc caaaagtact ttggtatgag tccagccacg
     4321 gcagaagcaa aagcaaacgc ttgtaagttg cccgttccaa catatcgcat cggcacatca
     4381 caaaaagcaa aacgttgcat caatattcaa gatcttgcgg aatacataga caaaaggcga
     4441 gaagaaggac gtatcgagtg ggaacaggtc agaacagtca aacagaagga caaagaacat
     4501 cactaaagaa aaaacccgcc tgaaggcggg ttttcaaaaa gcaccagcta tgatcatgct
     4561 gctttgcgac gacgaagctt accctgctgc tctttaccag agacagtagc gtgagtgaac
     4621 gcattaggag cagccttcat cagaacttca acagcagcac ccatacctgc gaatgctttc
     4681 attgtgtcga acttaacctg tggcttggtt gctttttgat ctttcataga aaactcccga
     4741 gacagtaaag gcgtctctaa ccctttcttt aaagctagct tgtttcgcta acttatgcca
     4801 atcgatcatg tcgattggtg acatcgtttc ttagtagttt aagcacaaaa cgactgccat
     4861 agatgtacct ttaaggtaat ctggacgggt atcctacaat ttgtagaccc ttctcgtcta
     4921 tacctactga gcaaatttaa gaaagatatc ctgcagctca tcaatgactg ccgacatcac
     4981 ataaccgcac tgttccatgc ggaaaccaaa agactcgtaa tactgcacca gttctggtac
     5041 tggctctaca atgtggacaa ctttacattc aacagcttta caaaatataa aagcactcat
     5101 aagagtgagt aaaaccatgc gccctttcaa tgggtgagat tcatcttctc tagaaaacct
     5161 ttcgatcata tggatacgaa agatgttttc ttcaacccca taaacacaaa ttgctgctcc
     5221 tgatggtatt ccctgaaccc gaccttgctg aacaagtttt atgcagaact catacttttc
     5281 tctggagttg ccataggtac ttaacgcata gtcccattca agctcaccat agccaccaca
     5341 cagaatcttg taatcatcat cactgagcgg accaacagca agaggtaagc cgacatgatc
     5401 aataatcaac tggatattgt tacgtactga ttgacctatc tcgtccaggg taagcatcat
     5461 ggcctctcaa gcggaacact aaaagtcgca ttatatctca ttcttaagcc gcgtatggat
     5521 tacaccttga aatgaaaacg ccgggttccc aataggctcc cacaaagtat ataactattt
     5581 gtttttcaaa aacggtacat cctatcgagc attggtgcaa cgctaaaccg accactccag
     5641 tgaacgtcag ttttttcagg cattgcgctg gtttggttga ttttttgcat ttcagaatta
     5701 ccgtgcattt tcaaatgtag agattatttt atcgatatat catgtggtta tgttattcag
     5761 catcactgtt caggaggctc aataacgggg tactatacca taacaacagg aagcgcctgt
     5821 ctcattgcaa aagaaaattg agatcctctc aaggcatgaa gctctcacga agtgaatatc
     5881 taatcttatt agatgttagc gttgtgaaaa gcaatgataa acaatacaga ttcgacgata
     5941 aataaaaatc acacattaaa ctccggtgat atctctccct gctaatagca ctgatagaga
     6001 aaaaaagaac ccaataaaca ttgggttctt tt
//
LOCUS       DBFIFI010000240         5906 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.240, whole genome shotgun sequence.
ACCESSION   DBFIFI010000240 DBFIFI010000000
VERSION     DBFIFI010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5906
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.240"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            87..1190
                     /gene="ompC"
                     /locus_tag="N7B39_004299"
     CDS             87..1190
                     /gene="ompC"
                     /locus_tag="N7B39_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HGU2424156.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVSGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQNF
                     EAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFNKNMSTY
                     VDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1302..2357
                     /gene="apbE"
                     /locus_tag="N7B39_004300"
     CDS             1302..2357
                     /gene="apbE"
                     /locus_tag="N7B39_004300"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HGU2424157.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            2431..3495
                     /gene="ada"
                     /locus_tag="N7B39_004301"
     CDS             2431..3495
                     /gene="ada"
                     /locus_tag="N7B39_004301"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HGU2424158.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYHKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3495..4145
                     /gene="alkB"
                     /locus_tag="N7B39_004302"
     CDS             3495..4145
                     /gene="alkB"
                     /locus_tag="N7B39_004302"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HGU2424159.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHNLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            4221..5864
                     /gene="yojI"
                     /locus_tag="N7B39_004303"
     CDS             4221..5864
                     /gene="yojI"
                     /locus_tag="N7B39_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HGU2424160.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPSWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELRNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT         1393 a         1506 c         1615 g         1392 t
ORIGIN      
        1 tggacttgcc gactgattaa tgagggttaa tcagtatgca gtggcataaa aaagcaaata
       61 aaggcatata acagagggtt aataacatga aagttaaagt actgtccctc ctggtcccag
      121 ctctgctggt agcaggcgca gcaaacgctg ctgaagttta caacaaagac ggcaacaaat
      181 tagatctgta cggtaaagta gacggcctgc actatttctc tgacgacaag tctgtagatg
      241 gcgaccagac ctacatgcgt cttggcttca aaggtgaaac tcaggttact gaccagctga
      301 ccggttacgg ccagtgggaa tatcagatcc agggcaacag cgctgaaaac gaaaacaact
      361 cctggacccg tgtggcattc gcaggtctga aattccagga tgtaggttct ttcgactacg
      421 gtcgtaacta cggcgtagtt tacgacgtaa cttcctggac cgacgttctg ccagaattcg
      481 gtggtgacac ctacggttct gacaacttca tgcagcagcg tggtaacggt ttcgcgacct
      541 accgtaacac tgacttcttc ggtctggttg acggcctgaa ctttgctgtt cagtaccagg
      601 gcaaaaacgg tagcgtaagc ggcgaaggca tgactaacaa cggtcgtgaa gcactgcgtc
      661 agaacggcga cggcgttggc ggatctatca cttatgatta cgaaggcttc ggtatcggtg
      721 ctgcagtttc cagctccaaa cgtactgatg atcagaacag cccgctgtac atcggtaacg
      781 gcgaccgtgc tgaaacctac actggtggtc tgaaatacga cgctaacaac atctatctgg
      841 ctgctcagta cacccagacc tacaacgcaa ctcgcgtagg ttccctgggt tgggcgaaca
      901 aagcacagaa cttcgaagct gttgctcagt accagttcga cttcggtctg cgtccgtccc
      961 tggcttacct gcagtctaaa ggtaaaaacc tgggtgtcat caatggtcgt aactacgacg
     1021 acgaagatat cctgaaatat gttgatgttg gcgcgaccta ctacttcaac aaaaacatgt
     1081 ccacctatgt tgactacaaa atcaacctgc tggacgacaa ccagttcact cgtgacgctg
     1141 gcatcaacac tgataacatc gtagctctgg gtctggttta ccagttctaa tctcgattga
     1201 tatcgaacaa agggcctgcg ggcccttttt tcattgtttt cagcgtacaa actcagtttt
     1261 ttggtgtact cttgcgaccg ttcgcatgag gataatcacg tatggaaata agctttaccc
     1321 gcgtggcact gctggctgcc gcgctcttct ttgttggttg cgatcaaaaa ccacaacccg
     1381 ccaaaaccca cgctactgaa gttaccgttc ttgaaggcaa aactatgggt accttctggc
     1441 gtgccagcat cccgggcatt gacgccaaac gcagcgccga acttaaagaa aagattcaga
     1501 cccagctgga cgccgacgat cagctgcttt cgacctataa aaaagattcc gcgctgatgc
     1561 gctttaacga ctcgcaaagt ttatcgccgt ggccggtaag tgaagcgatg gccgatatcg
     1621 tcaccacctc gctacgcatt ggcgcgaaga ccgatggcgc gatggatata accgtcgggc
     1681 cgctggtgaa tctgtggggt ttcggtccgg aacaacagcc ggttcaaatt ccgagccagg
     1741 aacagatcga cgcgatgaaa gccaaaactg gtttacagca ccttacggtc attaatcagt
     1801 cgcatcagca atatctgcaa aaagacctgc cggatttata tgtcgatctc tccaccgtcg
     1861 gcgaaggtta tgcggcggat cacctggcac gcttgatgga gcaggaaggg atttcccgct
     1921 atctggtgtc ggtgggcggc gcgctgaaca gccgtggtat gaacggtgaa ggcctgccgt
     1981 ggcgggtagc gattcaaaaa ccaaccgata aagaaaacgc ggttcaggcc gtggtggata
     2041 tcaacggtca tgggattagc acctctggca gctaccgtaa ctattacgaa ctggacggca
     2101 aacgtctttc ccatgttatc gatccgcaaa ccgggcgtcc catcgaacac aatctggtat
     2161 ccgtgacggt gattgcaccg acggcgctgg aagccgacgc ctgggataca ggtttgatgg
     2221 tactcgggcc ggagaaagcc aaagaagttg ttcgccggga agggctggcg gtttatatga
     2281 tcaccaaaga aggcgatagc tttaaaacct ggatgtcacc acagtttaaa agcttccttg
     2341 tcagcgaaaa aaattaaagc gcaagattgt tggtttttgc gtgatggtgg ccgggcagac
     2401 taaaggctat ccttaaccag ggagctgatt atgaaaaacg ccacatgctt aactgacgat
     2461 caacgctggc aatctgtctt agcccgcgac ccgaatgccg acggcgaatt cgttttcgcc
     2521 gtgcgcacca caggcatctt ttgccgtccg tcttgccgcg ccagacatgc tttgcgggaa
     2581 aacgtctcct tctacgcaaa tgccagcgag gcactcgccg ccggatttcg cccctgcaaa
     2641 cgctgccaac ctgataaagc caatgcccag caacatcggt tggataaaat cacccacgcg
     2701 tgtcgactgc tggaacagga aacgcctgta acgttggaag ccttagccga ccaggtggcg
     2761 atgagtccat ttcatctaca tcggttgttt aaagcgacta ccggaatgac gcctaaagcc
     2821 tggcaacagg cctggcgcgc tcgccgtttg cgcgaatcgc tggcgaaagg ggagagcgtg
     2881 acgacgtcta ttcttaacgc cggattcccc gacagcagca gttactacca caaagccgat
     2941 gaaacgctgg gcatgacggc taaacaattc cgtcacggtg gcgaaaatct ggcggtgcgt
     3001 tacgcgctgg ctgattgtga gctgggtcgt tgcctggtgg cagaaagcga gcgggggatt
     3061 tgcgcgatat tgctgggcga tgatgacgcg acattaatca gcgagttgca gcagatgttt
     3121 cccgctgccg acaacgcgcc tgccgatctg acgtttcagc aacatgtgcg tgaagtgatc
     3181 gccagcctca atcaacgcga tactccgctg acgttaccgc tggatattcg cggcactgct
     3241 tttcagcaac aagtctggca ggcactgcgc acgatacctt gcggtgaaac cgtcagttat
     3301 cagcaactgg ctaacgccat cggcaaaccg aaagcggtac gggccgttgc cagcgcctgt
     3361 gccgccaaca agctggctat cgtaattccc tgtcatcgag tggtccgtgg tgatggcaca
     3421 ctttccggtt accgctgggg cgtgtcgcgt aaagcgcaac tgctgcgccg cgaagctgaa
     3481 aatgaggaga ggtaatgttg gatctgtttg ccgatgctga accgtggcaa gagccactgg
     3541 cggctggtgc ggtaatttta cggcgttttg cttttaacgc tgcagagcaa ctaatccgcg
     3601 atattaatga cgttgccagc cagtcgccgt ttcgccagat ggtcaccccc gggggatata
     3661 ccatgtcggt ggcgatgacc aactgtgggc atctgggctg gacgacccat cggcaaggtt
     3721 atctctattc gcccattgat ccgcaaacaa ataaaccgtg gcccgccatg ccgcagagtt
     3781 ttcataattt atgtcaacgt gcggctacgg cggcgggcta tccagatttc cagccagatg
     3841 cttgtcttat caaccgctac gctcctggcg cgaaactgtc gctgcatcag gataaagacg
     3901 aaccggatct gcgcgcgcca attgtttctg tttctctggg cttacccgcg atttttcaat
     3961 ttggtggcct gaaacgtaat gatccgctca aacgtttgtt gttggaacat ggcgatgtgg
     4021 tggtatgggg cggtgaatcg cggctgtttt atcacggtat tcaaccgttg aaagcggggt
     4081 ttcatccact caccaccgac tgccgctaca acctgacatt ccgtcaggca ggtaaaaaag
     4141 aataaaaata agaattatta ttgctgtgcg cgcgaagatg tttaaactgc gggctgtaat
     4201 tcattgtccg ggttttctgc atggaacttc ttgtacttgt ctggcggcag tatcgctggc
     4261 catttatcag tgtgatggcg ctaagcctcg ccagtgcggc tttaggcatt ggcttaattg
     4321 cttttatcaa tcagcgcctt atcgaaacgg cggataccag tctgctggtg ttgccggagt
     4381 ttctgggatt attgctgctg ttgatggcag tcactctcgg atcgcaactg gcgctcacca
     4441 ctttggggca tcacttcgtt taccgactgc gtagtgaatt tatcaagcgg attctggata
     4501 ctcacgtcga gcgcattgaa caactcggta gcgcctcgtt gctggcggga ttaaccagcg
     4561 atgtgcgcaa tatcaccatt gcttttgtgc gtctgccgga actggtgcag gggatcattc
     4621 tcactatcgg ttcagcggcg tatctgtgga tgctgtcggg caaaatgttg ctggtaacgg
     4681 ctatctggat ggcagtcacc atctggggcg gttttgtgct ggtggcgcgg gtgtacaaac
     4741 atatggcgac cctgcgtgaa accgaagaca agctgtacac ggattttcaa actgtacttg
     4801 aagggcgcaa agagctgact ctgaaccggg aacgcgccga gtatgtgttt aacaacctct
     4861 acattcctga tgcgcaagag tatcgccatc atattattcg cgcagacacc ttccatctta
     4921 gtgccgtgaa ctggtcaaac atcatgatgc tgggcgctat cggcctggtg ttctggatgg
     4981 cgaacagtct cggttgggct gataccaacg ttgccgcgac ctattcgttg acgcttttat
     5041 tcctgcgtac gccgctgctt tcggcggttg gcgcattgcc gacgctgctg acggcgcagg
     5101 tggcgtttaa caagctgaac aaattcgcgc tcgcgccttt caaagcagag tttccgcgcc
     5161 cgcaggcgtt tcccagctgg caaacgctgg agctgcgtaa cgtgacgttt gcttatcagg
     5221 ataacgcgtt ttccgttggt ccgattaatc tcaccatcaa acgtggcgag ctgctgtttc
     5281 tgattggcgg caacggtagc ggaaaatcga cgctggcgat gttgttgacg ggcttgtatc
     5341 agccacaaag cggcgaaatc ttgctggatg gcaaacctgt cagcggcgaa caaccggaag
     5401 attatcgcaa actgttttcg gcagtgttta ccgatgtctg gctgtttgat caactgctgg
     5461 ggccggaggg taaacccgct aacccgcaac tggttgagaa gtggctggcg cagctgaaaa
     5521 tggctcataa gcttgagtta agaaacgggc gtattgttaa cctgaagtta tcaaaagggc
     5581 agaaaaaacg cgtggcgctg ttgctggcgc tggcagaaga acgcgatatt atcctgctgg
     5641 atgaatgggc ggcggatcag gatccacact tccgtcgtga gttttatcag gtgttgctgc
     5701 cgctgatgca ggagatgggt aaaactattt tcgccatcag tcatgatgat cattacttta
     5761 tccacgccga ccgcctgctg gaaatgcgca acgggcaact tagcgagctg acgggcgaag
     5821 agcgcgatgc cgcttcgcgt gatgccgttg cccggacggc ataatattac gacgctgacc
     5881 tggcggactg ctgccgccag gtcaat
//
LOCUS       DBFIFI010000241         5903 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.241, whole genome shotgun sequence.
ACCESSION   DBFIFI010000241 DBFIFI010000000
VERSION     DBFIFI010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5903
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.241"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(171..629)
                     /locus_tag="N7B39_004304"
     CDS             complement(171..629)
                     /locus_tag="N7B39_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU2424161.1"
                     /translation="MTLRKLFGQRVKELRIATGMSQEAFADRCGFARSYMSRIERGGS
                     NASLDAIEVLANALSVEPWRLLVSDSSEDSDPELLVPYAADGSCFHPGLASTRDGSFG
                     VGDKTAQKRFGTFSEALEYLRSMETAKWRRPNPSGNWGIVSAVRWDKLRK"
     gene            752..1525
                     /locus_tag="N7B39_004305"
     CDS             752..1525
                     /locus_tag="N7B39_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424162.1"
                     /translation="MSKTRLSKPGFSAKSVLTIMLIAASSASYAAESIDQLATRIGMD
                     HFREAIKKGERSAFKTLPAKGYKNGLTGLLIKTNTYVNPEYSFADCDTDTVIISVQEH
                     KTLPGCRISLKLATKDADGSYHDNGIRVEYGIAIVSKKFIANNSAWAQHAISGDKVLE
                     GALHPDKTNNNKDQMNNNKDQMCMGMAQFTANVTEGRNSGMTKREYYQRLDNMNGKAD
                     VTQQMITLYKTFVDYVYEHPKQDAKISGTYIYTNCMNNF"
     gene            1677..2993
                     /locus_tag="N7B39_004306"
     CDS             1677..2993
                     /locus_tag="N7B39_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424163.1"
                     /translation="MNDGVMLDSDVIAGLEWLASTQENKQHFYQRLAKAQQFYIATTQ
                     KAANFGKQFDPEWYGSDVVAGYFAQAKSLIDNRRSYEVTNASNIIPWVKQLGVCAKSL
                     DRITGARDRAIRMLKNTTVLPDTALLELVLAGNYASEGYEVEFIPEQKGIAKTPEFRC
                     RLGDGDYFFVECKRLQKGPYVKQEILQHHIRSHLAELHIKSKKMNVWTDVTYLCEVKD
                     APENYIISHLKNFKGVFYEWNDDYGKGTIRPANLNLIREDITENGSLLVNTKLARLIK
                     GSPLEDENYQVVAMGRPDERDSRFITKVKLASLITWRCINEKSFDARSRHVTKTLAEI
                     DNQLLDYGLGVGHIALDVDIQKDVADKRREKNYAAIVNFEQKSKIVRLNVHYLVPRID
                     ENSAWMVDETADDFFTHDLVKYVIPLTKIFPEAEVFENDQPGWHQN"
     gene            complement(3192..3761)
                     /locus_tag="N7B39_004307"
     CDS             complement(3192..3761)
                     /locus_tag="N7B39_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HGU2424164.1"
                     /translation="MAIFGYGRVSTSQQDTENQRMELEQAGWTFDFWFSDVVSGKVPA
                     VQRKAFFEMLSKIRDGETLVVAKLDRLGRDAIDVLQTVRTLADRNIKVIVHQLGTTDL
                     TSAAGKLLLSMLAAVAEMERDLLIERTNAGLLRAKAEGRKLGRPAKIAPEARGAILDK
                     RAAGVSVSALAREYGVSRATLAALLNKGY"
     gene            3954..4421
                     /locus_tag="N7B39_004308"
     CDS             3954..4421
                     /locus_tag="N7B39_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HGU2424165.1"
                     /translation="MIKDAIFSPCGKYRYSLSRVWDESKPYTLFIGLNPSYADAEKDD
                     RTLSRCISFAKSWGYGGVYMANLFAFVHTQRHEMMKASDPIGKDNDSHLIRLVSGAGL
                     VVAAWGNEGRHLKRSTTVRQLLPESTMCFVLNATGEPKHPLYMKNDSVLIPLG"
     gene            5425..5757
                     /locus_tag="N7B39_004309"
     CDS             5425..5757
                     /locus_tag="N7B39_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HGU2424166.1"
                     /translation="MRTVEILQYTLRKGSGAAFHAIMQEISVPLHQSHGIDVVSFGNS
                     LDDLDCYYLIRAFDSAKSMTAVLDAFYASADWRSGPREDIIGSIENCIKTVISLPSES
                     VKGLRMQS"
BASE COUNT         1730 a         1368 c         1314 g         1491 t
ORIGIN      
        1 gccattcagc gtcaaagtaa aacgccaccg taacgcctac gtaacgcacc cgcattttgc
       61 tgttttattc actcttatag aaacaaagag ctgatgaaac gtgaggctat cagcatctaa
      121 tgaacactgt tcatccatac agcatgaaaa ttcataaact tacacttgag ctactttctc
      181 agcttgtccc accgaaccgc agatacaata ccccagttgc cgctaggatt aggcctacgc
      241 cattttgctg tctccatgct acgaagatac tcaagagctt cagaaaacgt gccaaaccgc
      301 ttctgagccg ttttatcacc tacgccaaac gagccatcgc gagtgcttgc cagcccggga
      361 tgaaaacacg agccatcagc tgcataagga accagtaatt ccgggtcact atcttcagat
      421 gaatcagaca ccaatagtcg ccacggctca acactcagag cattagccag cacctcaatc
      481 gcatccaaag acgcattaga gccaccgcgt tcaatgcggc tcatatagct acgagcaaaa
      541 ccacaccgat cggcaaaggc ttcttggctc attccggtgg caatgcgcaa ctcttttact
      601 cgttgtccaa acagttttct cagggtcatt ttcatgacct gaatttgcgc agatgtccac
      661 taaagagcca cgctccaata gtgacatttt gaatcaatgg tctatgatgc gataactgac
      721 accgctgggg tggattctga gggataacaa catgtcaaaa acacggctta gtaagcccgg
      781 tttttcagcc aagtctgtgc tgaccattat gttaatcgct gcgtcatctg catcttatgc
      841 agcagaaagc atcgatcaac tggcgacgcg catcggtatg gatcacttcc gggaagcgat
      901 caaaaagggt gaacgcagcg ccttcaaaac gctgccggca aaaggctata aaaacggtct
      961 taccgggctg ttaatcaaaa ctaacactta cgttaacccc gaatacagct ttgctgattg
     1021 cgatacggac accgttatta tctcggtgca agaacacaaa acgcttcccg gttgccgtat
     1081 atctttaaag ctagcgacaa aagacgcaga tggcagttat cacgacaatg gaattcgcgt
     1141 tgaatacggt attgccatcg tctccaaaaa gtttatcgct aacaattcag cgtgggcgca
     1201 acatgccatc agcggtgata aagtgttgga aggcgctctc cacccggata aaacgaataa
     1261 caataaagat caaatgaata acaataaaga tcaaatgtgc atgggtatgg ctcaatttac
     1321 cgcaaacgta accgaaggcc gtaacagtgg gatgacgaaa agagaatatt atcagcgact
     1381 tgataacatg aacggtaagg ccgacgtcac ccagcagatg ataacgctct ataagacttt
     1441 tgttgactac gtatatgaac atccaaagca agacgcaaag atttcaggga cgtatattta
     1501 tactaactgt atgaacaact tctagaaacg taggtgcctg aacatgatag aactcagctg
     1561 ccagcataga gtggaaagcg gaggcttacc atcgtatatt catcgcgtta tctttccgta
     1621 actataatct gtctcagttg atctagtgcc tttcagggct gaactaagga ataaagatga
     1681 atgacggtgt tatgctagat tctgacgtta ttgctggtct tgagtggtta gcatcaactc
     1741 aagagaacaa acaacacttt taccagcgct tagcaaaagc acagcaattt tacatagcta
     1801 caacccaaaa agctgccaat ttcgggaaac agtttgaccc agaatggtat ggttccgacg
     1861 tggttgctgg ttatttcgca caagcaaaat cgcttatcga taatagaagg tcttacgaag
     1921 ttactaatgc ttcaaatata ataccatggg ttaaacaatt aggagtgtgt gctaaatcgc
     1981 tcgatagaat tactggcgct agagatcgcg caatcagaat gcttaagaat acaacagtat
     2041 taccagacac agccttatta gagttggttc ttgctggaaa ctatgcatca gaaggttatg
     2101 aagttgagtt cattccagaa caaaaaggga tagcaaaaac cccggagttt cgttgccgcc
     2161 ttggagatgg cgattatttc tttgtcgaat gcaaaaggct tcagaaaggt ccttacgtaa
     2221 agcaggaaat attacagcat catatacgct cacatcttgc cgaacttcat atcaaatcca
     2281 aaaagatgaa cgtatggact gatgtcacat atttatgtga agttaaagac gcgcctgaaa
     2341 actatatcat tagccactta aaaaacttta aaggtgtatt ttatgaatgg aatgatgatt
     2401 atggaaaagg aacgattaga ccggccaact taaatcttat cagggaagat atcactgaaa
     2461 acggctcatt actagtcaac actaaacttg ctcgtcttat caaaggctcc cctctggaag
     2521 atgaaaatta ccaagttgtt gctatgggac gaccagatga gcgcgactca cgtttcataa
     2581 ccaaagttaa acttgcttct ttgataacat ggcgttgtat caatgaaaaa tctttcgatg
     2641 cacgttcgcg acatgtaaca aaaacattag cagagataga taaccagctt ttggattatg
     2701 gtctaggcgt agggcatata gctttggatg tagatattca aaaggatgtt gccgataaac
     2761 gaagggaaaa aaactatgct gccatagtaa attttgagca aaaatctaaa atagtccgcc
     2821 tgaatgttca ttatctcgtg ccacgcatag atgagaactc tgcgtggatg gtagatgaaa
     2881 ctgcggatga cttttttacc catgatctgg tcaaatatgt tatcccattg actaaaatat
     2941 tccccgaggc tgaggtattt gagaatgacc aacctggctg gcatcaaaat taatttattt
     3001 ttgatagtat gaacgagggt agcatttcta tacaaatcag tgttcggctg atagcgagtt
     3061 aaacagttta ctgaaatgtt ctcagcaatt agctgttgcc aggacctcaa aaatccgatt
     3121 ctggcggaaa gtgtgctgtt ttaccaagta ttgctcgtat ctgcaaagtg ttagagcatg
     3181 ttttaagtat atcagtaacc cttattcagt aatgcagcaa gcgtcgctct tgaaacaccg
     3241 tattcacgcg ccaatgcgct aacactaacc ccagcggccc ttttgtctaa aatcgctcca
     3301 cgagcttccg gtgcgatttt agcaggcctg cccaatttcc tgccttcagc ttttgcccgt
     3361 aacagccccg cattagtccg ttcaatcagc aggtcacgct ccatctccgc aaccgctgct
     3421 agcatcgaca acaggagctt accggcagca gaggtgagat cagtggtgcc aagctgatgg
     3481 acaataacct tgatgtttcg gtcagccaac gtcctgacag tttgcagaac gtcaattgca
     3541 tcacgcccga ggcggtccag ctttgcaact accaacgttt ccccatcgcg gattttgctg
     3601 agcatttcaa aaaacgcttt gcgttgcacc gcaggaacct ttccgctgac tacatcagaa
     3661 aaccaaaagt cgaacgtcca cccagcttgt tcaagttcca tgcgctggtt ctcagtatcc
     3721 tgctgggatg tcgaaacacg cccgtagcca aaaatagcca tagtcatctc ctcatccggc
     3781 ccctgccaga aaacggtgat gaaaatagga tggcctgccg gaaaagccaa ggctaatttt
     3841 ccaacagtgt gaaagcgtgc ggttggagga tgccggaaaa cggtcgtttg ttggcgctta
     3901 aaacgggtga agacatgtta ggctgaaacc atcacttcat aaggacaacc gaaatgatta
     3961 aagacgcgat tttttcaccg tgcggtaagt atcgctactc gttatcacga gtctgggacg
     4021 aatcaaaacc gtataccctt ttcatagggc taaacccttc atacgccgat gccgagaagg
     4081 atgatcgcac cttgagccgc tgcatatcgt ttgccaaaag ctggggctac ggtggcgtat
     4141 acatggccaa cctgttcgca ttcgttcaca cccagcgaca tgaaatgatg aaagcgtctg
     4201 atcccattgg caaagataac gatagccact tgatacgtct tgttagcggt gccggactgg
     4261 ttgtcgccgc atggggaaat gaaggaagac acctcaaacg ctcaacgacc gtccgacagc
     4321 tattgccaga gagcacaatg tgcttcgtct taaacgctac aggtgagccg aagcaccctt
     4381 tgtatatgaa aaacgacagt gttctcatac ctttaggtta acatcgataa ctgcgttcag
     4441 aatatgaacg cagccatatg atatatatga cttttcttta aaatgatggt gtgctagctt
     4501 ctctcctgac acctacctca tgcggtaaac cggtccgggt aggtggagat caccgctgtt
     4561 tagcggaaga cgtcaggatg ggaactctgc gtaacgttga ttgtggtagg tagggcgcgc
     4621 aagcgtttga tgcagccaag cagagggtga aagtagctat cggcggattc ttaagtagct
     4681 tcttttgcgc ataatgcatc tttcaagaaa ggtcgtcaca ccacgaggaa gacttgatct
     4741 tcatctggca aggggaaccg agcaaaccta caggctatct tctcagatgg ccttagggtt
     4801 gatatgtccg gcttcccccc cgaggaaggg tttacatctt aaagaacaag ttatgtttta
     4861 acatttttaa caattacact tctctgctgt ttggtgtctt atggcgttcc cgtcaaaagt
     4921 aaagctgccg tttttacctc acatgcgtaa caaccaaaca cgaaccagaa gctgctaacg
     4981 ttcaacgtgt tcgcggtgta aatgcagccg gataagttgg gagcgaaaaa gtggatgtcc
     5041 taggtttgga agcgcccctc ttaagcattg catccgataa tttccgtggc ggtgagacag
     5101 tccttataga ggcaatcagc ctccaaagtt aagcgacgcg gtaaaactaa agctcgccca
     5161 gatttctcat tgattgctcg aactccgcta cgcttgtttc aactggcttc accagtcact
     5221 ctatgagagc gccgcgaatt ctgcaccatg atttcgacta ccttctttaa aacactcgcc
     5281 agagagcctt acagcgcgtt tgaaccgcat atcgcagctt ctgcttacgg atgactgtct
     5341 gattagcagt agcttcatgg cccagtccgg tttactttat tgctatcaac taggagccgc
     5401 ccaagggcaa cacggagata acgcttgaga accgtcgaaa tactgcaata caccttacgc
     5461 aaaggcagcg gtgccgcttt tcacgccata atgcaggaaa tcagtgttcc cctacaccaa
     5521 agccacggga tcgatgttgt ttcgtttggc aactcgcttg atgacctgga ttgctactac
     5581 ttgattcgcg cctttgacag cgctaaaagc atgactgcgg ttctcgatgc tttttacgca
     5641 agcgcagact ggcgcagcgg cccacgcgaa gacatcattg gcagcataga gaactgcatt
     5701 aaaacagtga ttagcctacc atcagaaagc gtgaaagggc tgcggatgca atcgtaagta
     5761 atcaagtgta ttagcttaga tttgacctaa acagctttgt gccaaaagtg gactaggaaa
     5821 agcctagcga taaagagttg agtttaataa ctactaactg aaatttaatg ggtgatatat
     5881 gaatttagat gaagaatttt acg
//
LOCUS       DBFIFI010000242         5872 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.242, whole genome shotgun sequence.
ACCESSION   DBFIFI010000242 DBFIFI010000000
VERSION     DBFIFI010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5872
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.242"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            134..781
                     /gene="fucA"
                     /locus_tag="N7B39_004310"
     CDS             134..781
                     /gene="fucA"
                     /locus_tag="N7B39_004310"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HGU2424167.1"
                     /translation="MERNKLARQIIETCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            809..1957
                     /gene="fucO"
                     /locus_tag="N7B39_004311"
     CDS             809..1957
                     /gene="fucO"
                     /locus_tag="N7B39_004311"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HGU2424168.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(2012..2767)
                     /gene="xni"
                     /locus_tag="N7B39_004312"
     CDS             complement(2012..2767)
                     /gene="xni"
                     /locus_tag="N7B39_004312"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HGU2424169.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(2879..4246)
                     /gene="sdaB"
                     /locus_tag="N7B39_004313"
     CDS             complement(2879..4246)
                     /gene="sdaB"
                     /locus_tag="N7B39_004313"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HGU2424170.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMCG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(4304..5593)
                     /gene="sdaC"
                     /locus_tag="N7B39_004314"
     CDS             complement(4304..5593)
                     /gene="sdaC"
                     /locus_tag="N7B39_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HGU2424171.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
BASE COUNT         1471 a         1468 c         1572 g         1361 t
ORIGIN      
        1 aggtcacaaa atataagctt gtgaatttct ttcttgttaa tcaggaagaa gatggtgggt
       61 aattaaacgg ctaattcaat agtgtgaaag gaacaacatt attgccctgt tttgaatcag
      121 agagaggtag ctaatggaac gaaataaact tgctcgtcag attattgaaa cttgcctgga
      181 aatgacccgc ctgggactga accaggggac agcggggaac gtcagtgtac gttatcagga
      241 tgggatgctg attacgccta caggcattcc atatgaaaaa ctgacggagt cgcatattgt
      301 ctttattgat ggcaacggta aacatgagga aggaaagctc ccctcaagcg agtggcgttt
      361 ccatatggcg gcctatcaaa gcagaccgga tgccaacgcg gttgttcaca atcatgccgt
      421 tcattgcaca gcagtttcca ttcttaaccg accgatcccc gctattcact acatgattgc
      481 ggcggctggc ggtaattcta ttccttgcgc gccttatgcg acctttggaa cacgcgaact
      541 ttctgaacat gttgcgctgg ctctcaaaaa tcgtaaggca actttgttac aacatcatgg
      601 gcttatcgct tgtgaggtga atctggaaaa agcgttatgg ctggcgcatg aagttgaagt
      661 gctggcgcaa ctttacctga cgaccctggc gattacggac ccggtgccag tgctgagcga
      721 tgaagagatt gccgtagtgc tggagaaatt caaaacctat gggttacgga ttgaagagta
      781 atttcgtaaa gcaacaagga gaaggatgat ggctaacaga atgattctga acgaaacggc
      841 atggtttggt cggggtgctg ttggggcttt aaccgatgag gtgaaacgcc gtggttatca
      901 gaaggcgctg atcgtcaccg ataaaacgct ggtgcaatgc ggcgtggtgg cgaaagtgac
      961 cgataagatg gatgctgcag ggctggcatg ggcgatttac gacggcgtag tgcccaaccc
     1021 aacaattact gtcgtcaaag aagggctcgg tgtattccag aatagcggcg cggattacct
     1081 gatcgctatt ggtggtggtt ctccacagga tacttgtaaa gcgattggca ttatcagcaa
     1141 caacccggag tttgccgatg tgcgtagcct ggaaggactt tccccgacca ataaacccag
     1201 tgtaccgatt ctggcaatcc ccaccacagc aggcactgcg gcagaagtga ccattaacta
     1261 cgtgatcact gacgaagaaa aacggcgcaa gtttgtttgc gttgatccgc atgatatccc
     1321 gcaggtggcg tttattgacg ctgacatgat ggatggtatg cctccagcgc tgaaagctgc
     1381 gacgggtgtc gatgcgctca ctcatgctat tgaggggtat attacccgtg gcgcgtgggc
     1441 gctaaccgat gcactgcaca ttaaagcgat tgaaatcatt gctggggcgc tgcgaggatc
     1501 ggttgctggt gataaggatg ccggagaaga aatggcgctc gggcagtatg ttgcgggtat
     1561 gggcttctcg aatgtggggt tagggttggt gcatggtatg gcgcatccac tgggcgcgtt
     1621 ttataacact ccacacggtg ttgcaaacgc catcctgtta ccgcatgtca tgcgttataa
     1681 cgctgacttt accggtgaga agtaccgcga tatcgcgcgc gttatgggcg tgaaagtgga
     1741 aggtatgagc ctggaagagg cgcgtaatgc cgctgttgaa gcggtgtttg ctctcaaccg
     1801 tgatgtcggt attccgccac atttgcgtga tgttggtgta cgcaaggaag acattccggc
     1861 actggcgcag gcggcactgg atgatgtttg taccggtggc aacccgcgtg aagcaacgct
     1921 tgaggatatt gtagagcttt accataccgc ctggtaaatg cgctgatgtg ataatgccgg
     1981 atgcgacgtt tgcggcgtat ccggcacgcc gttaccgtac caaccgcaat tgctgtaaat
     2041 tgccgtcgat atgcaaatcg gtttgtaagc gggcaatatc gcggcacaga aacgccatct
     2101 ctttatgggt ttctaatttt ttgcgccact tttcggcaac cgcatccaga ttctcatata
     2161 tcccttccag actctgaaac tcgaccagca gctgcgtggc gctttttggt ccgattcccg
     2221 caacacccgg tacctttgaa ctgctgatcc ccgcaagtcc ccagtaatcg ggcaactgct
     2281 gcggttgaac gccaaattct ttatcgataa atggcgcatc cagccaacgt ttctggaagt
     2341 aatcacgaat acgtaatgtc ggtgaaagta actgacagta gcctttatct gtcgaaacaa
     2401 tcgttgcctg atgcccggcc tgtgtcactt tgaccgccag cgtggcggct aagtcatcgg
     2461 cttcgttgcc gctggttgac cagcacggga cgccgcgttg ctcaaaggcg gcgcgtaatg
     2521 caggcatctc gtcgtgcaac tcttccggca ttggcggtcg acccgctttg taatctggta
     2581 aacgctgatg acgccagccg ctactgcggt tttcatcgtc aaaaacggcg accgcgtggg
     2641 ttggctggct gtgcataatg agctgatcga gcgcatgctg gcaggtttcg acacagggcg
     2701 acccctgaac ggcatgaatg cgacgaataa gattcagtgc atcgacaata agcaaatgaa
     2761 cagccacgat aaccccctta aagatcaggc gataagggta acattcgata ctggaacagg
     2821 ctatggctgg atgagaaatc gggaagaggc ctcgcaaaac gaggcctctg gagagcgatt
     2881 aatcgcaggc aacgatcttc attgccaggc cgccgcgaga ggtttcgcgg tacttggcgt
     2941 tcatatcttt acctgtttcg tacatggttt cgataacttt atcgaggcag acgcgcggct
     3001 cgctggtacg gcgcagcgcc atacgtgcgg cgttcaccgc ttttactgcc gcaatggcgt
     3061 tacgctcgat gcatggcacc tgtacctgtc cggcgaccgg gtcacacgtc agaccgaggt
     3121 tgtgctccat ggcgatttcc gccgcgatgc acacctgcgc cgggcttgca cctaacagtt
     3181 ctgccagacc agccgccgcc attgagcacg ccacaccaac ttcaccctgg caacccactt
     3241 cggcaccaga aatcgacgcg ttcatcttat aaagagaacc aatggcgctg gctaccagca
     3301 ggtaacgagc cagtgagtta gcgttcactt cgcggataaa cttatcgtag tacgccagca
     3361 ctgccgggat aatcccgcac gcaccgttag tcggcgcagt caccacgcga ccgccagcag
     3421 cgttctcttc gttcactgcc agtgcaaaca tgttgatcca gtcaacaacc gccatcgggt
     3481 cagtggtggt tttatcctgg ctgaccagca tccggcgtag tgccgcagca cggcgtggaa
     3541 cacgcagttt gccaggtaac acaccttcgg tggaaatacc gcgctcaata ccgccacaca
     3601 tcacttccca gacgttcgcc aggtgctgtt ccagctcttc tttgctgtgc agcgccagct
     3661 cgtttttcat catcaggcca gagagtgaca gcccggtttc ctgacaatgt ttttgcagat
     3721 cggctgctga actgtacgga taaggaactt caaccggtgc gctatcctgc tggccaaaat
     3781 gctcttcatc aacgataaag ccaccgccaa tagagtagta agtctgactg taaacgactt
     3841 tatcacccgc cagcgcggta atgcgcatac cgttttcatg cagagaaagg ttgtcggcat
     3901 ggaagttcat gcactgatca accgggaact ccacttcatg ctgaccgttt gccagcatca
     3961 ggcgaccatg agtattcaca tcctgaataa aaccagggat ggaatcgata tccacggtat
     4021 ccggcaggtt acccgccagg cccataataa tggcgatatc agtgtggtgg cctttaccag
     4081 tcagagagag cgagccgtac acgtcaacca ccacgcgggt cacgtcttta agcaggttac
     4141 gggcaatcag atcgtcggta aattgtttac ccgctttcat tggtccaacg gtatgagaac
     4201 tggaagggcc aatgccgatt ttgaaaatat cgaatacgct aatcatagga aatacatcgc
     4261 gttaaaacgg aggaagcgcc gcccgaaagc ggcgcgaaag gacttagctg aacagagagt
     4321 agaagattgc ggagattgca atcagaccca tcacgacaac gaatacgttg ctgatgtgac
     4381 cgctgtactt acgcattgcc ggtactttct gaattgcgta catcggcatc aggaacagga
     4441 tcatcgcgat gattgggccg cccagggttt caatcatacc caggatgctc gggttcaggg
     4501 tggcaacaat ccaggtcgtt accagcatga acagcgcagt gatacggttc agcttgttga
     4561 tttcgataga cttaccttta ccacgcagag atttaatcac cataccgttg aagccttcac
     4621 gtgcgcccag gtagtggccg aggaaggatt tggtgatagc gataatcgcg ataatcggag
     4681 ccatccacgc gataaccggt gcgttaaagt ggttagccag gtaagacaga atcgagatgt
     4741 tctgctcttt agccgcagcc aggtctgccg gagtcaggct cagtacgcag ctgaagacga
     4801 agaacattac ggtcagcacc atcatgatgt gtgcgaatgc caggatcttc gagcatttct
     4861 gttctgccat atcgccgtac tcttcacgct tcgcaacggc gaaagaagag atgatcggag
     4921 agtggttgaa cgagaacacc attaccggaa ttgccagcca cagggtcatc cacagaccgt
     4981 ttccggttgc agatgcagtg tccagagaca gcgtttccag tgctgcgccg ttccactgcg
     5041 ggatcaggta cagagccagc agcatcagta cgccaacaaa cgggaatacc agaatactca
     5101 tcgctttaac gatcatctgt tcaccaaagc gaacgatggt catcataccc acgatcagga
     5161 tcagcgacag aatcgcacgc ggcggtggtg tcatacccag ctggtgagac atgaagcttt
     5221 caactgtatt ggtgattgcc acgctataaa ccagcaggat cgggtagata gcgaagaagt
     5281 agagcagggt aatcagttta cctgcgccaa taccaaagtg ttcttctaca acctcggtaa
     5341 tgtcttcgcc cgggttttta ccagacagta cgaagcgagt caggccgcgg tgagcaaaaa
     5401 acgtcatcgg gaacgcaagg atagccatga tgatcagcgg gatcataccg ccaacaccgg
     5461 cgttgattgg caggaacagc acgcccgcgc cgattgccgt gccgtaaagg cccagcatcc
     5521 acatggtgtc tgtcttgcgc caggcactac gagagtcttt cgacgcaatc gtgctggttt
     5581 gagtcgtttc catctatttc tcctggagga atgcaaataa ttcagctttt agccagttca
     5641 gcccgggaag gtgcctgacc gtaaaaatga aatctgtcta tcggcgatgg cttaatcatt
     5701 taccgccgta ataatttgag ggcggaaaga tacacacaag ttatgtatct atcagtgatc
     5761 ttgatcccaa atacaataat ctacttggaa gatagattat cccaacgctt tactctgtaa
     5821 atcaaaagta aaagagaatt taagggcaat atttgcccat taaacgacat ct
//
LOCUS       DBFIFI010000243         5853 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.243, whole genome shotgun sequence.
ACCESSION   DBFIFI010000243 DBFIFI010000000
VERSION     DBFIFI010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5853
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.243"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(205..594)
                     /locus_tag="N7B39_004315"
     CDS             complement(205..594)
                     /locus_tag="N7B39_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424172.1"
                     /translation="MEFKDLLKEIQEIAAHALHQRLNEVELESATKKYIDNMARNVRD
                     AFTGLYSVSVTNNQNTEETAKRIASVMGFHVEEKYSKKEFWKTTKKLQSENCHLLRQS
                     LLSMRKVIQMTQDYRNCSHYLKNSELS"
     gene            complement(606..734)
                     /gene="ydfB"
                     /locus_tag="N7B39_004316"
     CDS             complement(606..734)
                     /gene="ydfB"
                     /locus_tag="N7B39_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfB"
                     /protein_id="HGU2424173.1"
                     /translation="MDFDTIMEKAYEEYFEGLAEGEEALSFSEFKQALSSSAKSND"
     gene            complement(736..891)
                     /gene="ydfA"
                     /locus_tag="N7B39_004317"
     CDS             complement(736..891)
                     /gene="ydfA"
                     /locus_tag="N7B39_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HGU2424174.1"
                     /translation="MDTIDLGNNESLVYGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            complement(1169..1456)
                     /locus_tag="N7B39_004318"
     CDS             complement(1169..1456)
                     /locus_tag="N7B39_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGU2424175.1"
                     /translation="MLPILWLPSARDDLRQIITYIAKENPPAARRLKIRIETSVLPLS
                     EHPYLYPPSERVSGLREIVTHPNYIILYRVAASSIEIVSVTHSRRQFPFSI"
     gene            complement(1456..1647)
                     /locus_tag="N7B39_004319"
     CDS             complement(1456..1647)
                     /locus_tag="N7B39_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HGU2424176.1"
                     /translation="MGTALSPIVSEFETTEQENSYNEWLRTKVTSSLADTRPAIPHDE
                     VMAEMENLIAQIAVTNKSE"
     gene            complement(1675..2076)
                     /locus_tag="N7B39_004320"
     CDS             complement(1675..2076)
                     /locus_tag="N7B39_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU2424177.1"
                     /translation="MMSALEVSMYRISKLLQETGWSQAELARRIGVTQQTVQQWVSGK
                     ATPKASSLDKLVEVSGHPLHWFLLPPEECEQIFTPDTMKIGPRQRELLQAFSAFPEED
                     QEKMLQEIKDKKKSMEETIARWLAAQKSRRA"
     gene            2185..2457
                     /locus_tag="N7B39_004321"
     CDS             2185..2457
                     /locus_tag="N7B39_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU2424178.1"
                     /translation="MLDSTREKIRQKYTQAEIGRYMGVAQQTVWQWFSFGVPPKQVIP
                     LCQLMKWEVTPHEIRPDIYPNPTDGLPVGCKVNTSNAPELIHENQA"
     gene            2441..2866
                     /locus_tag="N7B39_004322"
     CDS             2441..2866
                     /locus_tag="N7B39_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HGU2424179.1"
                     /translation="MKIKHEHIRMAMNAWAHPDGEKVPAAKITKAYFELGMTFPELYD
                     DSHPEAMARNTQKIFRWVEKDTPDAVKKIQALLPAIEKAMPPPLVARMRSHSSAYFRE
                     LVETKERLVKDIDDFVASAIVLFDQMNRGGPAGNTLAVH"
     gene            complement(3073..3528)
                     /locus_tag="N7B39_004323"
     CDS             complement(3073..3528)
                     /locus_tag="N7B39_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424180.1"
                     /translation="MYFLKSLYQAHVLNVAATNRWCNSPEMLPDYRAWLRAETYLRLD
                     ILISELQKETASIHNLQGIDAVRILVSRHSALSIIEVRHLSFSELIFLLQPALESANI
                     PPEVIQYPPHVDEQLQDVPYNQRAGLTPCSEAEWDHSLLKKYQDLYNPQ"
     gene            3607..4698
                     /locus_tag="N7B39_004324"
     CDS             3607..4698
                     /locus_tag="N7B39_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGU2424181.1"
                     /translation="MRDYAKVSPRFWLGETGRELRKAGAEAQVVAFYLMTSPHANMLG
                     LYYLPVLYLAHETGLGLEGASKGLKRAVEAGFCSYDHDAEMVWVHEMAAWQVGETLKP
                     GDNRCAGVRNEYASLPENAFLSVFYDRYKTDFHLDVRRNNSRNSVRGFEGAFKGLRSQ
                     EQEQEQEKEQEQDKNTMVHGKKNTTNQAGDVQTVNPGQPAGTTPEADSGAVQQVMTAG
                     SEQSHQLQQPEADSAIQREADRVVPESTGQSVGRVDYPDVFEQVWREYPLRAGANPKK
                     SAFSAWKARLREGVPPETMLDGVRRYARYLAATGKAGTEFVQRATTFFGPDRNFENPW
                     LLPVSGTNNQRCVNHISEPDTEIPPGFRG"
     gene            4705..5451
                     /locus_tag="N7B39_004325"
     CDS             4705..5451
                     /locus_tag="N7B39_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGU2424182.1"
                     /translation="MKNIAAAGVLERIRRLAPQASVPPYRTVEEWREWQLAEGRKRSE
                     EINRQNHQLRVEKILNRSGIQPLHSKCSFANYQVQNDGLKYALSQAKSIADELMTGCT
                     NFVFSGKTGTGKNHLAAAMGNRLMAKGRSVIIVTVSDVMSVLHDSYDNGKSGEKFLQE
                     LCSVDLLVLDEIGVQRETKNEQVVLHQIIDRRTASLCSVGMLTNLNHAAMSTLLGERI
                     MDRMTMNGGRWVTFNWDSWRPNVSNMRVVK"
     gene            5473..>5853
                     /locus_tag="N7B39_004326"
     CDS             5473..>5853
                     /locus_tag="N7B39_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HGU2424183.1"
                     /translation="METVSDALKALKKASSHVVAARLGISREEAVNELWELKINGVVD
                     KTGHTWFLAGEGESRVTEERPVKSEAQDMLTGEVEQKVTADMMIEFIGQDGAKTCEEL
                     AGKFGVSTRKVASTLAVVTATGRLA"
BASE COUNT         1527 a         1259 c         1560 g         1507 t
ORIGIN      
        1 aacgccgccc gagattaagg gcgtagtggc ccattaaaaa tattgcaaag accaactatt
       61 gttgaactgg attaaaccag taattctcta ttgctcttat tgttaccatc ggatgattgc
      121 cgggggttat tttaagttcg ggaggaaaag gctctccatc agcagccatc attgagtatg
      181 tgaaatccgc cgacgcagtc cctttcatga taattccgaa tttttcaagt aatgagagca
      241 gttccggtaa tcctgtgtca tctgaataac tttcctcata gaaagaagcg attgccgcag
      301 gagatgacag ttctcgctct gcaatttttt tgtagttttc cagaattctt tttttgaata
      361 cttttcctcg acatgaaaac ccatcaccga ggcaatccgc tttgcagttt cttcagtatt
      421 ctggttgttt gttaccgaaa cagagtacaa tccggtaaac gcatcgcgca cattacgagc
      481 catattatca atgtatttct tcgttgcaga ttctaattca acttcgttca gacgttggtg
      541 aagtgcatgt gctgcaattt cctggatttc ttttagtaaa tctttaaatt ccatctttaa
      601 ctccgttagt cgttagattt tgccgaactg gaaagcgcct gtttaaactc actgaagctg
      661 agagcttcct cgccttcggc aaggccttcg aagtattctt cgtaagcctt ttccatgatt
      721 gtgtcgaaat ccatatcact cacctgagtt tctttccagc cagcgacggg caccattttc
      781 ggttttaaac gttttgcttt tggtatacgt catcgcggtg aatgtaccgt cctggttggg
      841 aaacacgccg tacaccagag attcgttgtt gccaagatcg atagtatcca tgttgacctc
      901 atttcccctt aacgccgggt agcggaacaa aaacctgctg catagttatt aaagttgaac
      961 cctgccgtca tgttcatacg cctcgggctg gctacttacc ccctgaccac tgcttggtaa
     1021 ctcgaagtat tgcccggcgt tctgtggggc ggggtgggtg gttggtgtat gtaatctaca
     1081 ataaaaaact gtttttgtgt caacagtttt taattgttgt tttgggcaaa aaaatccctc
     1141 gaaagaggga gtatgaaaat tgttcaactc agatagagaa gggaaattgt cgccgagaat
     1201 gtgtcacgct tacaatctca atgcttgaag cagctactct gtacaggatt atgtagttag
     1261 ggtgggtcac gatctctctc aatccagaaa cccgttcgct tggtggatat aagtacggat
     1321 gctcagatag aggtaatacc gatgtttcaa tgcgtatttt tagtctacgt gctgccggtg
     1381 ggttctcctt ggcgatgtaa gttatgatct ggcgcaaatc atcgcgagca gacggtagcc
     1441 ataaaatggg taacattact cgctcttgtt agttacagca atttgagcaa taagattttc
     1501 catttcagcc attacctcgt catgtggaat tgcggggcga gtgtctgcaa ggcttgacgt
     1561 tactttagtg cgcaaccatt cgttgtaact gttttcttgt tcggtagttt cgaattctga
     1621 aactatcgga gaaagggctg tacccatggc ataactcctc ttcttgtact gtggtcacgc
     1681 ccggcggctt ttttgtgccg ccagccacct agcaatggtt tcttccattg attttttctt
     1741 gtctttgatt tcttgaagca ttttttcttg gtcttcctca ggaaacgcac taaaagcttg
     1801 gagcagttcg cgttggcgag gaccaatttt catcgtgtca ggggtgaaaa tttgctcaca
     1861 ctcttcagga ggcaataaaa accaatgcaa tggatgccct gaaacctcaa ccagtttatc
     1921 caaacttgag gctttaggtg tagccttacc gctgacccat tgttgaacag tttgttgtgt
     1981 cacaccaatt ctacgggcaa gctcagcctg gctccatcca gtttcctgaa gaagcttgct
     2041 gattctgtac atagatactt ctagggcgct catcattatt caattttaca ggtaaatact
     2101 gttaaaagca tcacaataaa aaactgttga ttgcatacag ttttttattg taggctttgc
     2161 ttatagtttt tagaggaggg caaaatgcta gatagcactc gcgaaaaaat taggcagaaa
     2221 tacactcagg ctgaaatagg tcgttatatg ggggtcgctc aacagactgt ttggcaatgg
     2281 tttagctttg gcgttccccc aaagcaggta attccgttat gccaactaat gaagtgggaa
     2341 gttaccccgc atgaaatccg cccagatatt tatcctaacc caaccgacgg tttacctgtt
     2401 ggatgtaagg ttaacacatc aaatgcgccg gagttgattc atgaaaatca agcatgaaca
     2461 catccgcatg gcgatgaatg cctgggcgca tccggacggc gaaaaagtac cggctgcgaa
     2521 aattaccaaa gcgtatttcg agctgggaat gacgttcccg gaactgtatg acgacagcca
     2581 tccggaagcc atggctcgca atactcagaa aattttccgc tgggtggaga aagacactcc
     2641 tgatgcggtt aaaaaaattc aggcgttgtt accagcgatc gaaaaagcga tgccgcctcc
     2701 gctggtggcc cgaatgcgca gccacagttc cgcttatttt cgggagttgg tagagacgaa
     2761 ggaacggctg gtgaaagata ttgatgattt cgttgcatca gcgatcgttc tgttcgatca
     2821 gatgaatcgt ggtggcccgg caggaaacac tctggctgtg cattaattgg gtaataaata
     2881 tgagtaatga caaaaaattg acactgagcg tttacgaaaa cagtccgcac atctggcgtg
     2941 gcggtttatc tgatgtggag ctggcagagt ggttgataca taaagctaat gcgctgctct
     3001 ggcgtttgtc agccagagaa cagcgcaagg aaaccagaat aaagctggct gatgcagaag
     3061 cgtgtgccgg gcttattgag gattatacaa atcttggtat ttcttcagca gagagtgatc
     3121 ccattcagcc tctgagcagg gagtcaatcc agcacgctgg ttgtatggca catcttgtaa
     3181 ctgctcgtca acatgaggtg ggtattggat cacttccggc gggatattcg ctgattccag
     3241 agctggttga agcaagaaaa tcagttcaga aaaagagaga tgacgcactt caattattga
     3301 gagagcacta tggcgcgata ccagaatgcg aacagcgtcg ataccctgaa ggttatgaat
     3361 ggatgcagtc tctttttgaa gttcgctaat caatatgtcg agacgaaggt atgtttcggc
     3421 gcgcagccag gctctgtaat ccgggagcat ttcggggctg ttacaccagc ggtttgttgc
     3481 tgcaacattt aatacatgag cctgataaag gcttttcaaa aaatacatgt cgaacctcct
     3541 ctggttctgt cgattgggaa ccacagatta tatccggagg aaggttcggc accagatgag
     3601 gtagccatgc gtgattacgc aaaagtttct ccgcgattct ggctgggaga aacggggaga
     3661 gaacttagaa aggcgggtgc agaagcgcaa gttgttgctt tttacctgat gacatcccct
     3721 cacgcaaata tgctgggttt gtattacctg ccagttttat accttgctca tgaaaccggg
     3781 cttggtctgg aaggggcttc aaaggggctt aaaagggctg ttgaagctgg tttttgtagc
     3841 tatgaccatg atgcagagat ggtctgggtc catgaaatgg cagcctggca ggttggggaa
     3901 acgttgaagc ctggcgataa ccgttgtgca ggtgtcagga atgagtatgc atcattacct
     3961 gaaaacgctt ttctgtcagt gttttacgac agatataaaa cggatttcca tctggatgtg
     4021 aggcggaata atagccgaaa ttcggtaagg ggcttcgaag gggcttttaa ggggcttcga
     4081 agccaagaac aggaacagga gcaggagaaa gaacaggaac aggacaaaaa cactatggtt
     4141 catggcaaaa aaaacaccac gaaccaggca ggggatgttc agaccgtcaa tcctggtcag
     4201 ccagcaggca cgacaccgga agccgattcg ggcgctgtgc agcaggtgat gaccgcaggg
     4261 tcggagcaat cacaccaact gcagcagcct gaagccgatt ccgccattca gcgggaagcc
     4321 gatcgggtag tcccggaaag caccgggcag tctgtgggac gagtggatta tccggatgtg
     4381 ttcgaacagg tctggcggga atacccgttg cgtgctgggg caaacccgaa gaaatccgct
     4441 ttcagtgcct ggaaggccag attgcgcgag ggggtgccac cagagaccat gctggatggt
     4501 gtgaggcgtt acgcgagata cctggcggcg accgggaaag cgggaacgga atttgttcag
     4561 cgagcgacga cgttttttgg gccggaccgg aattttgaaa acccctggtt gctcccggta
     4621 agcggcacga acaaccagcg ttgtgtgaat catatttctg aaccggatac cgaaattccg
     4681 ccgggattca gggggtgatg tggcatgaaa aacattgcgg cagccggggt tcttgaacgt
     4741 attcgcagac ttgcaccaca ggcgtcggtt ccaccgtacc ggacggtgga ggagtggcgg
     4801 gaatggcaac ttgctgaagg acgaaaacgc agcgaggaga ttaaccgcca gaatcaccag
     4861 ttgcgggtgg aaaaaatcct gaatcgttcg ggcatccagc ctctgcacag caaatgctcg
     4921 tttgcgaatt atcaggtgca gaacgacggg ctaaaatacg cgctgagcca ggccaaatcc
     4981 atagctgacg aactgatgac cgggtgcacg aattttgtgt tcagcggtaa aaccggcacc
     5041 gggaaaaatc accttgcagc ggcgatgggt aaccggctga tggcgaaggg gcgcagcgtg
     5101 attatcgtca ccgtgtctga tgtcatgagc gtgttgcatg acagctacga caacggcaaa
     5161 tccggggaaa aatttttaca ggagctttgc agtgttgatt tgctggtcct ggatgaaata
     5221 ggcgttcagc gggagacgaa aaacgagcag gtggtattac accagataat tgatcgccgg
     5281 acagcatcac tgtgcagtgt cgggatgtta acaaacctga atcatgccgc aatgagtaca
     5341 cttcttggtg agaggattat ggaccgcatg accatgaacg gtggtcgatg ggtgacgttt
     5401 aactgggata gctggcgtcc aaatgtcagc aatatgaggg ttgtgaagta attttgtccg
     5461 gaggaaattt taatggaaac cgtatctgac gcactgaaag cactgaaaaa agcctcttca
     5521 catgtggtgg cagctcgcct tggaatcagt cgtgaagagg ctgtcaacga gctgtgggaa
     5581 ctcaaaataa atggcgtcgt tgataaaact ggtcacacct ggtttctggc tggcgaaggt
     5641 gaatcccggg taaccgaaga gcggccagta aaatctgaag cacaggatat gctgaccggg
     5701 gaggtcgaac aaaaagttac cgcagacatg atgattgagt ttatcggtca ggatggggct
     5761 aaaacgtgtg aggaactggc gggtaagttc ggtgtcagta ctcgcaaggt tgcttccacg
     5821 ctggcggtgg taaccgcaac ggggcggctg gca
//
LOCUS       DBFIFI010000244         5815 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.244, whole genome shotgun sequence.
ACCESSION   DBFIFI010000244 DBFIFI010000000
VERSION     DBFIFI010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5815
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.244"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            62..508
                     /locus_tag="N7B39_004327"
     CDS             62..508
                     /locus_tag="N7B39_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HGU2424184.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATMALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSTCVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQEFIHQLQQGDTDQVLALLNQTLSS"
     gene            519..1958
                     /locus_tag="N7B39_004328"
     CDS             519..1958
                     /locus_tag="N7B39_004328"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HGU2424185.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAIPYAL
                     AAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLAIGLA
                     LNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            1948..3018
                     /locus_tag="N7B39_004329"
     CDS             1948..3018
                     /locus_tag="N7B39_004329"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HGU2424186.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSREEAKKRGAEIGDFISPEAN
                     FACWGEDKIVGKALDNRIGCAMMAELLQTVNNPGITLYGVGSVEEEVGLRGAQTSAEH
                     TKPDVVIVLDTAVAGDVPGIDNIKYPLKLGNGPGLMLFDKRYLPNQKLVAALKNCAAH
                     NGLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQVD"
     gene            3018..4766
                     /locus_tag="N7B39_004330"
     CDS             3018..4766
                     /locus_tag="N7B39_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HGU2424187.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARISASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EHNGLFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGLASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(4816..>5815)
                     /locus_tag="N7B39_004331"
     CDS             complement(4816..>5815)
                     /locus_tag="N7B39_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HGU2424188.1"
                     /translation="TGCDDKKAETETPLPANSQPAAPAPEAKPTEAPVAKADAKPETP
                     AQPLVDEQAVFDEKMDVYIKCYNKLQIPVQRSLARYADWLKDFKQGPTGKESTVYGIY
                     GISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNTINEMDKYYTQENYK
                     DDAFAKGKTLHQTFLKNLEAFEPVAESYHAAIQEINDKRQLAELKNIEEREGKTFHYY
                     SLAVMISAKQINNLISQEKFDVDAAMKKVSELETLVAQAKEADKGGMNFSFINSADQY
                     QLETKKYVRRVRDKVPYSDWDKEHLQDANTSWMVEDSFPRALREYNEMVDDYNSLR"
BASE COUNT         1352 a         1480 c         1474 g         1509 t
ORIGIN      
        1 tcatcacaaa cccgccccgc tattacaatt aactgaaata ttttttcatg gagtccgatt
       61 aatggcagct cttactgcaa gctgtattga cctgaatatt cagggcaatg gcgcttattc
      121 cgttctgaag cagttggcga caatggcgtt acaaaacggt tttatcaccg actcacacca
      181 gttcctgcaa accctgctgc tgcgcgaaaa aatgcactcc accggttttg gttctggtgt
      241 cgccgtgccg cacggtaaaa gcacctgcgt taaacaaccg ttcgtgttat tcgcccgcaa
      301 agcgcaggct attgactgga aagccagcga cggcgaagac gtcaattgct ggatctgcct
      361 cggcgtaccg caaagtggcg aagaggatca ggtcaaaatc atcggcacac tgtgtcgcaa
      421 aattattcac caggaattta ttcatcaact gcaacagggc gataccgacc aggtgcttgc
      481 cttgttaaat caaaccctca gctcataagg aagtggcgat ggagtcatcc ttacgtattg
      541 tcgcgatcac caactgcccc gccgggatcg ctcacaccta catggtggcg gaagccctgg
      601 aacagaaagc ccgttctctc ggccatacca ttaaagtgga aactcaaggg tccagcggcg
      661 ttgaaaaccg cttatccagc gaagagattg ccgctgccga ttacgtgatt ctcgccaccg
      721 ggcgtggcct gagcggtgac gatcgcgcac ggtttgccgg gaaaaaagtt tatgagattg
      781 ccatctccca ggcgctgaaa aatatcgatc agattttcag tgaattaccg acaaattcgc
      841 agctttttgc cgcagatagc ggcgtgaagc taggcaaaca ggaagtacaa agcggcagcg
      901 tgatgagcca cctgatggcg ggcgtctctg cggcgctgcc gtttgtcatc ggcggtggta
      961 tcctggtggc gcttgccaac atgctggtgc agttcggttt gccgtatacc gatatgtcga
     1021 aaggcgcgcc gtcgtttacc tgggtagttg aatccatcgg ttaccttggc ttcaccttta
     1081 tgatccccat catgggcgct tatattgcct cgtcgattgc cgataaaccg gctttcgctc
     1141 cggcgtttct ggtttgctat ctggcgaacg acaaagcgct gctcggcacc cagtcgggtg
     1201 cgggattcct cggcgcggtg gtgctggggc tggcgattgg ctatttcgtc ttctggttcc
     1261 gtaaagtccg tcttggcaaa gcattacaac cgctgctcgg ctccatgctt atcccattcg
     1321 ttactctgct ggtttttggc gtgctcactt actacgttat cgggccggtg atgtccgacc
     1381 tcatgggcgg gctactgcac ttcctgaata ccattcctcc atcaatgaag tttgcggcag
     1441 cgtttctggt cggtgcgatg ctggcgttcg atatgggcgg ccccatcaat aaaaccgcct
     1501 ggttcttctg cttctcactg ctggaaaaac acatttatga ctggtacgcc atcgtcggcg
     1561 ttgtccccgt cgcggcgggt cttgcgacgt tcattgcgcc caaactgttt acccgacagg
     1621 aaaaagaggc tgccagtagt gccattgtgg tcggtgccac agtcgcaacc gaaccggcta
     1681 ttccttacgc tctggcagct ccgttgccga tgattaccgc caatacgctg gcgggcggca
     1741 ttaccggcgt gctggtgatc gctttcggaa tcaaacgtct ggcacctggt ttgggtatct
     1801 ttgacccgct gatcggcctg atgtcgccgg taggttcgtt ttatctggtg ctggcgattg
     1861 gcctcgcgct gaacatttca ttcattatcg tcctgaaagg attgtggtta cgccgcaaag
     1921 cgaaagccgc gcaacaggag cttgtccatg aacattgagt tactgcaaca gttgtgcgaa
     1981 gccagcgccg tcagcggcga tgaacaggaa gttcgcgaca ttctgataaa cacgctggaa
     2041 ccttgcgtta atgagatcac ctttgatggt ctgggcagct ttgttgcccg taagggaaat
     2101 aaaggtccaa aagttgccgt tgtcgggcat atggatgaag tcggctttat ggtcacccac
     2161 atcgacgaga gcggttttct gcgctttacc accattggcg gctggtggaa tcagtcgatg
     2221 ctcaaccacc gggtaacaat acgcacacac aagggagtga aaatccctgg tgtgattggt
     2281 tccgtagcgc ctcatgcgtt aacggaaaag caaaagcaac aaccgctgtc atttgatgag
     2341 atgttcattg atattggcgc gaacagtcgc gaagaagcga aaaaacgcgg tgcggaaatt
     2401 ggcgatttta ttagcccgga agccaatttt gcctgctggg gcgaagataa aatagtcggc
     2461 aaggcgctgg ataatcgcat cggctgcgcg atgatggctg agctactaca gacagtaaat
     2521 aacccaggaa ttacgctgta cggcgtcggc agcgtggaag aagaagttgg gctacgcggg
     2581 gcacaaacct cggctgaaca cactaaaccg gatgtggtga tcgtgctgga taccgccgtc
     2641 gcgggcgatg ttccgggcat tgataacatt aaatacccgc tgaaactggg caacgggccg
     2701 gggctgatgc tgtttgacaa gcgctactta cccaaccaga aactggtggc ggcgttaaaa
     2761 aactgtgccg cacataacgg tttaccgctg caattttcca ccatgaaaac cggagcgacg
     2821 gatggcgggc gctacaacgt aatgggcgga gggcgtccgg ttgtcgcgct gtgtctgcca
     2881 actcgttatc tgcacgctaa cagcggtatg atttcaaaag ccgattatga tgctctgctc
     2941 acgctgatac gggattttct gacgacctta actgcggaga aagtcaacgc gtttagccag
     3001 ttccgtcagg tggattaatg ctaaacgaac gccagttaaa gattgtcgat ctgctggagc
     3061 aacagccgcg cacgcctggc gagctggcgc aacagactgg cgtttcaggc aggaccatcc
     3121 tgcgtgatat tgactatctc aactttaccc ttaacggcaa agctcgtatc tctgccagtg
     3181 gcagtgcggg ctatcagttg gaaatcttcg agcgccgcag cttttttcag ttgctgcaaa
     3241 agcacgataa cgacgatcgg ctgctggcgc tgttattact gaataccttt actccccgtg
     3301 cgcaactcgc ctcggcgctt aatttgccag aaacttgggt cgcagagcgt ctgccccggt
     3361 taaaacagcg ttatgaacgc acttgttgcc tggccagtcg tcccggtttg ggccatttca
     3421 ttgatgaaac agaagagaaa cgcgttatct tgctggcgaa cttgctacgc aaagatccgt
     3481 ttttaatacc tttggctggc gtaacacgag acaaccttca gcatttatcc acggcctgcg
     3541 acaaccaaca ccgctggccg ctcatgcagg gggattatct ctccagcctg attctggcga
     3601 tttacgccct gcgtaatcaa ctgaccgatg agtggccgca atatcccggt gacgagataa
     3661 aacaaatcgt tgaacataac ggtctgtttc ttggtgataa cgctgtaaga accctgacag
     3721 gtttgataga gaaacagcat cagcaagcgc aggtaatttc tgccgatcat gtgctggggt
     3781 tgctgcaaag ggtgccaggt ctcgcgtcat tgaatattat tgatacgcag ctggttgaga
     3841 acattaccgg tcatttatta cgttgccttg ccgcaccagt gtggattgct gagcatcgcc
     3901 aaagcagcat gaataacctg aaagccgcct ggcctgcggc ctttgatatg agtctgcact
     3961 ttattacgct actgcgtgaa cagctcgata ttcccctttt cgacagcgat ctgatcggtt
     4021 tgtattttgc ctgtgcgctg gaacgacatc aaaacgaacg ccagccgatc attttgctct
     4081 cggaccagaa cgcgattgcc actattaatc agctcgccat tgagcgcgat gttttacatt
     4141 gtcgggtgat tattgcccgt agcttaagcg aacttgttgc cattcgcgaa gagattgagc
     4201 cgttattgat cattaacaac agccattatt tactggatga cgcggtaaat aattacatca
     4261 ccgtaaaaaa tatcattacg gctgccggta tcgaacaaat aaaacatttt ctggcgacgg
     4321 catttattcg ccagcaaccg gagcgttttt tctctgcccc cggaagtttt cattattcga
     4381 atgtacgcgg tgaaagctgg caacatatta cccggcaaat ttgtgcgcaa ttagtcgcac
     4441 aacaccatat taccgccgat gaagcgcaac ggatcatcgc ccgcgaaggc gaaggtgaaa
     4501 acctgattgt taatcgcctc gccatcccac attgctggag cgaacaggag cgacgttttc
     4561 gtggattttt tattaccctc gcccaaccag ttgaggtgaa taacgaagtc attaaccatg
     4621 tcttgatcgc ctgcgccgcc gccgatgcgc gtcatgagct aaaaatattt agctatctgg
     4681 caagcgtatt gtgtcagcat ccggcagaag ttattgccgg gctaacagga tatgaggcat
     4741 ttatggagtt acttcacaag gggtgaactg tgtcggatgc gccgcatccg acactgcatt
     4801 tatcatcaaa aacgtttaac gcaggctatt atagtcatca accatttcgt tgtactcgcg
     4861 taacgctcgt ggaaatgagt cttcgaccat ccagcttgtg tttgcatcct gaagatgctc
     4921 tttatcccag tcagagtacg ggactttatc tctgacgcgg cgaacatatt ttttagtctc
     4981 gagttgatac tggtctgccg aattaatgaa cgagaaattc atgccgcctt tatccgcttc
     5041 tttggcctgc gccaccagcg tttccagttc agacactttc ttcattgccg catcaacatc
     5101 aaacttctct tgcgatatca ggttattaat ttgtttcgct gaaatcatga ctgccagaga
     5161 atagtagtgg aatgttttcc cttctctttc ttcaatattt ttcagttccg caagctggcg
     5221 cttatcatta atttcctgaa tcgccgcatg ataagattct gctacaggtt caaaggcttc
     5281 cagatttttt aagaatgtct ggtgcagcgt tttccctttc gcaaacgcat cgtctttata
     5341 attttcctgg gtgtaatatt tatccatttc gttaatggta ttacccaacg ccacggcagc
     5401 atcgatataa ctcaccgcca cgccatcaat tggttgcagc gcaggcgtta acgccacagc
     5461 actttttacg cctttttcac actcagcgag gttggattca ctaatgccgt agatgccata
     5521 aacagtactc tctttaccgg taggcccctg tttaaaatct ttcagccagt cagcataacg
     5581 cgccagactg cgctgtaccg ggatctgtaa cttgttgtag cacttgatat agacatccat
     5641 tttttcgtcg aaaaccgctt gttcatcgac cagcggttgc gcaggtgttt caggtttagc
     5701 atctgctttt gcaacgggag cttcagtagg tttcgcttca ggagctggtg cggcaggttg
     5761 actattggca ggcaggggcg tttctgtttc ggctttttta tcatcacaac ccgtc
//
LOCUS       DBFIFI010000245         5777 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.245, whole genome shotgun sequence.
ACCESSION   DBFIFI010000245 DBFIFI010000000
VERSION     DBFIFI010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5777
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.245"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            2..1021
                     /gene="tsuA"
                     /locus_tag="N7B39_004332"
     CDS             2..1021
                     /gene="tsuA"
                     /locus_tag="N7B39_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001647169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HGU2424189.1"
                     /translation="MLGFVMQRGRFCLTGGFRDMYIAKNNRMFYALLIAISVQSVGVF
                     ALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRAGEGLIGSWIALFT
                     YMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVAVLLVITLWVVMKELK
                     KPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLSEATGRMFGLGITS
                     PTANILQFLVAGDVKYINWGVFLVLGIFVGAFIAAKASREFRVRAADAQTTVRSGLGG
                     VLMGFGASIAGGCSIGNGLVMTAMMTWQGWVGLVFMILGVWTASWLVYVRPQRKARLA
                     TAAAN"
     gene            1035..1262
                     /gene="tsuB"
                     /locus_tag="N7B39_004333"
     CDS             1035..1262
                     /gene="tsuB"
                     /locus_tag="N7B39_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HGU2424190.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(1305..2729)
                     /gene="sbcB"
                     /locus_tag="N7B39_004334"
     CDS             complement(1305..2729)
                     /gene="sbcB"
                     /locus_tag="N7B39_004334"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HGU2424191.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDSD
                     TLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYAEEIQMLA
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            2911..4089
                     /locus_tag="N7B39_004335"
     CDS             2911..4089
                     /locus_tag="N7B39_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU2424192.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKTQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIE
                     RSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLLDNVWTVPKEHSKMGNVIRRPIF
                     DQIKPFLEKAMTTYNDVLFPGEDINKPISIAAANRFVNRIRGGMDLGYWRTHDFRRTL
                     VTRLSEMNVEPHVTERMLGHELGGIMSVYNKHDWIEAQRKAYELHADKLFWHIRSISD
                     "
     gene            complement(4070..4261)
                     /locus_tag="N7B39_004336"
     CDS             complement(4070..4261)
                     /locus_tag="N7B39_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001576843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU2424193.1"
                     /translation="MTDTSLIPEKEVMNKLGVSSRQTIWNYTKRHGFPKPVRTHPKSY
                     LREAVEGWILNGGVNQKCS"
     gene            complement(4339..4683)
                     /locus_tag="N7B39_004337"
     CDS             complement(4339..4683)
                     /locus_tag="N7B39_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424194.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHGFGAPH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKIHGNKAP
                     ETYRRISELRKD"
     gene            complement(4871..5221)
                     /locus_tag="N7B39_004338"
     CDS             complement(4871..5221)
                     /locus_tag="N7B39_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HGU2424195.1"
                     /translation="MINRIKLEHILEYARQQRHIGQHCKIPPGDMVEIMEIAMRKAGN
                     SPVTPDGWISCSERMPEKYDFDIWVFSPSRGVLDGLQWDGSMFTDDEYQFVIHDATHW
                     MRKVYPAAPQQDGE"
     gene            complement(5218..5574)
                     /locus_tag="N7B39_004339"
     CDS             complement(5218..5574)
                     /locus_tag="N7B39_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424196.1"
                     /translation="MTTITDKELIKEIKERIGSLDVRDNIERRAYEIALASLEAEPIA
                     WECGENIILFNPDTVEAYAKRAEISPKPLFSAPPALVVPDKLPREYRNGWPLAYSDYA
                     EGWNDCREAMLQGDKS"
     gene            complement(5571..>5777)
                     /locus_tag="N7B39_004340"
     CDS             complement(5571..>5777)
                     /locus_tag="N7B39_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424197.1"
                     /translation="VVNGRVRKVHGWPKGNCCWGNPDDKPFTGNKQVECDYMLSALRR
                     MELRGHLNEIYDGVIVNKKEGNAA"
BASE COUNT         1406 a         1401 c         1415 g         1555 t
ORIGIN      
        1 tctgctggga tttgtcatgc agcgtgggcg tttttgcctg actggtggtt ttcgcgatat
       61 gtatatcgcg aaaaataatc gcatgtttta cgccttgctg attgcaattt cggtacaaag
      121 cgtgggggtt tttgcgttaa ttcaggcggg cctactgact tacgaagccg gggcgttccc
      181 gtggcttggt actgttatag gtgggtatct cttcgggctg ggaattgttc tggcgggtgg
      241 atgtgccacc gggacctggt atcgcgcggg tgagggattg atcggcagtt ggatcgcgct
      301 tttcacttat atggtgatga gtgcggtgat gcgttctcca cacgccagtg gtttaaatca
      361 aaccttgcag cactacacta ctgaacataa ctctattgct gagactttta atttgtctgt
      421 gtggccgttg gttgccgttt tgctggtgat aacgctctgg gtggtgatga aagagttgaa
      481 gaagccaaaa ctcaaagtcg cgaccttacc gccgcgccga accgggatcg ctcatattct
      541 gtttgaaaaa cgctggcatc cctttgtcac agctgtactc atcggtttga ttgcgctttt
      601 agcctggccc ctgagtgaag caaccggacg tatgtttggg ttaggcatca cttccccaac
      661 ggccaatatt ctgcaatttc tggtcgcggg tgacgtgaaa tacattaact ggggcgtttt
      721 cctggtgtta gggatcttcg tgggggcatt tattgcagcc aaagcgagcc gtgagttccg
      781 cgttcgcgca gctgatgcac aaacaacagt acgtagcggg ctaggtggtg tactgatggg
      841 cttcggtgcc agtattgcag gtggttgctc tatcggtaat ggactggtta tgactgcaat
      901 gatgacctgg cagggctggg ttggccttgt atttatgatt ctcggagtct ggactgcgtc
      961 ctggcttgtg tatgttcgac cgcagcgtaa ggcgcgactg gcaactgctg cggcaaatta
     1021 agtaaggtgt aaagatggcg attaaaaagc tggatgtggt gacgcaggtc tgtccgtttc
     1081 cactcataga agcaaaagca gcactggcag aaatggccag cggcgatgag ttggtaattg
     1141 agtttgattg cacccaggca actgaagcaa tcccacagtg ggcggcagaa gaagggcatg
     1201 ccatcaccga ttatcagcag attggcgatg cagcgtggag catcaccgtt caaaaagcct
     1261 gactcaacat tgtcctccgc cgtaccagcg gcggaggctt cacattagac aatctcttcc
     1321 gcgtactgcc aaagcgcttt taacagcgcc actttttctt tatcgtcggc atactgctgc
     1381 gccagcatct gaatctcttc tgcatagcct tgtaaaaact ctggcgtgaa gacctggcgg
     1441 cggtgctcca gccagcgttg ctgctcggca taatccagcg tccccgggaa gttgcgtgcc
     1501 cgataattga acaacagctt ttcaatccgt ttatcaacaa aagtgatatc cagtgccggt
     1561 aaattacgcg gctcggtttc aagcacaatt ttcattgctg cacgatcggc gtcactgaaa
     1621 aagccattat aaagctgtgc atccacgtta tctgaaggcg taaacggttc ggcttccgcg
     1681 aatatcgcca ccactttttc acgcacttgc ggattttcac gcagaatttt caggttatcg
     1741 aggcaatgct gacgattaat tcccagtcgg tcggcatctt ccgggcgtag cgtattcgcc
     1801 tgggccagca ccggacattt attgatatgt accagtttaa ccggaacggc ggcgttatcg
     1861 ccaagatcgg tttttgcggt atataaacgc tcgcgcaatg tgtcgctatc cagttccagt
     1921 aatggcgaaa tatctcctgc caaatccacc ataattacgg cattgcgatt ttcaggatgc
     1981 cacgccagcg gtgccaccca gctggtattg ccgcgccatg ccccaaacat tccggaaacg
     2041 tgaaccaggg gtttcatctg cggaacatca atcaacgcca tcagtttgtg tttattacga
     2101 tgggtaaaga gataatcaaa caggcgtggt tgacgcgttt ttaccagttt cgccatcgca
     2161 atagtggcgt acacatcagc catcgcatcg tgggcgttgc tatgttcaat accattcgct
     2221 ttggttaaat gctcaaggcg aaagctcggt agaccgtcat cattttcagg ccagtttatt
     2281 ccttccgggc gcagggcata gcaggcacgc ataacatcca gtaaatccca gcgcgagtta
     2341 tcatgctgcc agctccaggc gtaaggatcg tagaaattac gataaaaaac gttgcgtgtg
     2401 acttcgtcgt cgaaacgcac attgttgtag cccagaatac aggtcttcgg tacggtaaaa
     2461 agcgagtgaa tacgggcggc aaatgcggct tcgttttctc ctttcgcccg agcttcctgc
     2521 ggggtaatac cggtaattaa tactgctcca ggctggggta aatagtcatc cgcgggcttg
     2581 cagtaaaaga cttcgggttc gccgatgaca ttgaattcgt catcggtgcg aatggctgcg
     2641 aactgtgcag ggcgatctaa cgcggggtgc gtgccaaagg tttcgtaatc gtgaaagagg
     2701 aaggtaggtt gcttatctgt gtctgtcatt gtctagcatt gtccaatgta gttaattaac
     2761 tgtctgttat aactcgaaaa acaacccttt tttcacctga gtatgtctac gaacgtcaaa
     2821 tatagttgtt tactgtgcgc tgttgtccat gtgatattgt gtaccagatg tgtaccaaac
     2881 cattatttct gagcgtacca aatattattt atggctataa gcgacacaaa actgcgtact
     2941 atttatggta aaccatattc tggcccacaa gaagtggctg atgccgatgg cctcagcgta
     3001 cgaatttcac cgaagggggt catccagttc cagtaccgct atcgctggca tggcaagcct
     3061 aatcgacttg ggcttggtcg atacccatcc ctgtctttga aggatgccag acagatcact
     3121 gctgacttgc gaaagctcta tttctcagga acggatccac gcacttattt tgaagagaag
     3181 gtggagaact ccatgacggt cgcccagtgt ctcgactact ggttcgacaa ctacgtctct
     3241 acaactctca gagaaaagac ccaagcactt taccgatcaa cggttatgaa gcgcatgcat
     3301 gacgccttcc ctaatcgtcc ggcatcttct atcacggtta agcaatgggt tgacctgctt
     3361 accgaagaag aaaaagataa tccacgccga gcaaggcagg tgctaagtca actaagatca
     3421 gcaattagtt ggtgcatgcg gcgtcagttg atagatagtt gcgcaattat gagcatccaa
     3481 ccaagggact tcggctcccg cgctgaggta ggggatcggg tactgtcgta tcacgaactg
     3541 gctaagatct ggcttgctat tgaaagaagc cgtgcgtcta cgtcaaataa gctccttcat
     3601 cagatgctta tgctgtgggg ggcgaggctc tcagagctta ggctggcaac aaagacagaa
     3661 tttgacctgc tggacaacgt atggaccgta ccgaaagagc atagcaagat gggtaatgtt
     3721 atccgccgtc caatcttcga tcaaattaag ccttttctcg aaaaggccat gacaacgtac
     3781 aatgatgttc ttttccctgg agaagacata aacaaaccga tcagcatcgc tgcagctaac
     3841 cgattcgtaa atagaataag gggagggatg gatctaggtt actggcgaac acatgatttc
     3901 agaagaacgc ttgttacacg tctgtccgag atgaatgtcg agcctcatgt tactgagcga
     3961 atgctcggtc atgaacttgg cgggataatg tccgtataca ataaacacga ctggatagag
     4021 gctcagcgca aagcgtatga gcttcacgct gataaattgt tctggcacat caggagcatt
     4081 tctgattaac gccaccgtta agaatccacc cttcaacagc ttcacgaagg tatgatttgg
     4141 ggtgggttct gactggcttc ggaaatccgt gccgtttggt atagttccag attgtctgac
     4201 gtgatgaaac accgagcttg ttcatcactt ctttctcagg aatcaggctg gtatcggtca
     4261 tcttaattct ccaggcaaaa agaaaccgcc atatagcggc tctatcagat atgaacaggc
     4321 ctcatcgagt gtgaggcgtt agtccttgcg tagctcgctg attcttctgt aagtctctgg
     4381 tgctttgttt ccgtgtatct tcatttcaga cttcaacaga gcaacgaggg aatcccattc
     4441 gttgaggatt cctttgaatg ccggaacgcg ctttgcaacc ttgtcgaatg aatctctgat
     4501 ttctggaatc tgctcaacaa gtgcaacgca tcgtctgaaa tcggctgcgt catgtggagc
     4561 accgaagcca tgaccataga tattcttttt caggccacat gcgattgagg caagagttgc
     4621 gctactgatg ccgacatcgc cagttgattg ccatttcaaa accttcatag ccaaatctga
     4681 catttcttgt ctccataaaa caaaactcgc cgtagcgagt tcagataaaa gaaatccccg
     4741 cgagtgcgag gattgttagt tgcgctctgc tgctgcctta gccattacca tatccacaca
     4801 atttcaccag acatgattct cgcaatcact atcatcacca aagtgataat cacaacttta
     4861 actggcggca tcattcacca tcctgctgcg gtgctgctgg atatactttt ctcatccaat
     4921 gcgtggcgtc atgaataacg aactgatatt catcatcagt aaacatcgag ccatcccact
     4981 gtagcccgtc aagaacacct ctggatggag agaataccca gatatcgaaa tcgtatttct
     5041 ccggcattcg ctcactacag cttatccaac catccggagt taccggagag ttgccagcct
     5101 tgcgcatggc aatctccatg atttcaacca tatctcctgg tggaatttta caatgctgac
     5161 caatatgcct ctgctgcctg gcatattcga ggatgtgctc cagcttgata cgattaatca
     5221 tgatttatct ccctgaagca tggcttcgcg gcagtcgttc cagccttcag cataatcact
     5281 atacgcaaga ggccaaccgt ttctgtattc acgcggcaac ttatcaggca ctaccagcgc
     5341 tggcggcgcg gagaatagtg gtttaggtga tatttccgca cgttttgcgt atgcttcaac
     5401 tgtgtcaggg ttaaacagga ttatgttttc accgcattcc cacgctatcg gttctgcttc
     5461 cagcgatgcc agtgcaattt cataagcacg gcgctcaata ttgtctcgaa cgtccaggct
     5521 gcctatgcgc tctttgattt ctttaatcag ttctttgtcg gtaatagtgg tcatgccgcg
     5581 tttccttctt tcttattaac aattacaccg tcatatattt cattaaggtg ccctctcaac
     5641 tccatgcgcc ttaatgcaga caacatgtaa tcgcattcaa cctgcttatt tccagtaaat
     5701 ggcttatcgt caggattacc ccaacagcaa ttacccttgg gccacccatg tactttccgt
     5761 actcttccgt taacaac
//
LOCUS       DBFIFI010000246         5758 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.246, whole genome shotgun sequence.
ACCESSION   DBFIFI010000246 DBFIFI010000000
VERSION     DBFIFI010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5758
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.246"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(10..2442)
                     /gene="metL"
                     /locus_tag="N7B39_004341"
     CDS             complement(10..2442)
                     /gene="metL"
                     /locus_tag="N7B39_004341"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HGU2424198.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPTSQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(2445..3605)
                     /gene="metB"
                     /locus_tag="N7B39_004342"
     CDS             complement(2445..3605)
                     /gene="metB"
                     /locus_tag="N7B39_004342"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HGU2424199.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAENPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAKAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            3882..4199
                     /gene="metJ"
                     /locus_tag="N7B39_004343"
     CDS             3882..4199
                     /gene="metJ"
                     /locus_tag="N7B39_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HGU2424200.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            4259..4867
                     /locus_tag="N7B39_004344"
     CDS             4259..4867
                     /locus_tag="N7B39_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HGU2424201.1"
                     /translation="MKIRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            5012..5758
                     /locus_tag="N7B39_004345"
                     /pseudo
     CDS             5012..5758
                     /locus_tag="N7B39_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558263.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         1368 a         1628 c         1489 g         1273 t
ORIGIN      
        1 tttctgaaat tacaacaact gtgccagccg gttgatatcc gactgaatcg ccccggcggt
       61 gacgtcgcgc cccgcgccag gcccacggat caccagcggg ttgtcgcgat accagcggct
      121 ttcgatggca aagacgttat cgcacggtag cagtgatgcc agcggatggt cttcacgcac
      181 cgcctccacg cccacacgcg ctttgccgtt ggcatcgaaa cgcgccacgt agcgcagcac
      241 cagccccatt tcgcgggccg cttccagccg ttgcaccatc tgctcgttaa gttcatcgcc
      301 attttcaaag aaatggtcga tgctgccgcc ttcgcaatga gcaggcacca gcgattccac
      361 acgtacctgg tccggttcaa tgttgtaacc cgcttcacgc gccagaatca ccagcttgcg
      421 cattacgtct ttgccggaga ggtcgtcacg cggatcaggc tcggttaagc cctgctgcca
      481 cgcctgatcc accagctcgg taaacggcac gctaccgtcg aattgcagga acagccagga
      541 gagcgtgccg gagaagatcc cactgatcga caaaatagta tcgccgctgt cgatcagatc
      601 gcgcacggta tggttgatcg gcaagcccgc accgacggtg gcgttgtata gccagtgacg
      661 cccggttttt tcgaaggcgt cgtggatctg gcgatattta ttgctgtcgc ttgctcccgc
      721 cagtttgttg gcactgatga cgtggaaacc gtggctggcg aaatcaagat actggtcagc
      781 cagctgctgg ctggcggtaa cgtccagcac cactaaatca tcatacggat gggcgcgcat
      841 ccacaggaac aacgactctt catcctgctc aaccgcttca tcgttgaaga aggctaacgc
      901 gcggctggcg tccagcccgt cataactcaa cagactgcgg cggctgtcca ccacgcctgc
      961 cagcacaaat tcaaagccgg tacgcgccga aagcgtgctc tgctcgcggg cgaacagttc
     1021 cagccagcgg gaaccgatat tgcctttgcc gaacaatacc aggccgatgc gtttttctgc
     1081 gcggaagacg gactgatgca ggccctgaat caggctttcg gtcgggccgg tgcgcagtac
     1141 tgccaccagg ctgatgccgt catcggactg ccaggtaaac tcgaccggct ggcctttcag
     1201 ttgctgccag aagcggtggc aatgcagcgg gttacgggtg acacccgccc cgaccatcgc
     1261 caccagcgcc agcccctgac gcaggcgcag ttcgccaggt aatcccgctt cgtcgaggat
     1321 tttcagcgca ctgtcggcca cttctgaggt gtagcaaaat tgcagcaact ggcgatcgtt
     1381 atgcacgcca accgccagtg ggcgcacctg agcacgtttc aggatctggt cgatctcttt
     1441 atgcgccagt ttgaaatcct ggctggtggg cacctgaaac tcaatcaagc agacatcatc
     1501 gtggctggtg acaatacgcg caccagtacc ggaggccagc acgcgttcaa tgcgcgtgga
     1561 gccttgatcc ggcgtgtagc tacagcgcag ctgcaggtcg atttcgctac cggatactgg
     1621 ctgtaaagta cgggcgtgaa gaacgggagc cgccagacgc gccagttcgc tggcttcatc
     1681 cagacgcagc aatggcagca gacaggcatc tttcacttta cgcggatcgg cactgtatac
     1741 cccggcgacg tcgctccaga tggttacacg agaaacaccc gccagcgcac cgatttgtgt
     1801 cgcggaatag tcggaaccgt tacgccccag cagcaccgtt tcaccggcgt tgttacggct
     1861 gataaacccg gtcaccacca gacgtttacc cggatgttgt accagtagct gttgcagcaa
     1921 cgggtaagaa agcccttcat caacctgcgg ttgcgcagcg cgttcagcgc gtaaaaactc
     1981 gcgggcatca agccaggccg ctggcagccc ttgttgatta agcaccgcag acatcaggcg
     2041 cgccgaccac acttcgccgt ggcccaccac ttccgcatac actgcgtcgt taataccgct
     2101 gtcgagcagc gccgccaggc gctcaagatc gctgacaaaa gcgctaatga ggctatcggc
     2161 ttcttcggcg ggtagcagac cgctaatcag atcgcactga tagcgacgca gcgtttgttg
     2221 aacctgatgc gcagagagac gatcggtctg gcttagtttc aaccagttaa tcaactggtt
     2281 agtggtgcta ccagcggcgg aaaccaccat catatcgtca ggctgagagt actccgccat
     2341 aatgcccgcg acacgcaaat aacacttcac atcagccaga ctactgccac caaatttatg
     2401 cagctgacga cctttcgccc ctgcctgcgc aatcacactc atttttaccc cttgtttgca
     2461 gcccggaagc cattttccag gtcggcaatt aaatcttcgc catcttcaat accggtggag
     2521 atacgcagca gcgtctcgga gatcccggcg gcagcacgcg cttctggtgc catgcctgca
     2581 tgtgtcatgg ttgcggcgtg agagattaaa ctttccactc cccctaatga ttccgccagc
     2641 gtaaataacg acagcccgcc caggaaacgg cgcagcgtct gctcatcgcc atccagttca
     2701 aaactcaaca ttgcgccaaa gcctttttgc tgacgcgcgg caatttcatg cccctgattt
     2761 tccggcagcg acgggtgata cagttttttc accaacggct gggtttgcag atatttcaca
     2821 atcgccttcg cgttgcgctg cgccagctcc atacgcggca ccagcgttcg caacccgcgt
     2881 agcagcagat agctgtcaaa cgcgccgccc gtcacgccaa tattgtttgc ccaccaggca
     2941 agttcagtga caacgtccgg gtctttagca atcaccacgc cagccaccac gtcagagtga
     3001 ccgttcagat atttcgtaca tgaatgcaac accagatcgg cacctaatgc cagcggattt
     3061 tgtaatgccg ggcttaagaa ggtgttatcc accacgctca ccgccccgac ttcccttgcc
     3121 agatggcaga ttttcgcaat atccacgacg cgtaacaatg gattacttgg gctttctacc
     3181 agtaccagtt tggggttttc tgccagcgct gcccgtaatg cctgttcatc gccttgatca
     3241 acaaacaaca cgcgatagca accgcgtttc gccaggctgt cgaacaggcg atagctaccg
     3301 ccgtagcagt cgtgcggcgc aaccagcaga tcgccaggtt tcaaaaagac ggtcgttacc
     3361 agatgaatcg cggacatgcc ggtattagta agtactgcac cagcaccgcc ttccagttct
     3421 gccagcgcac gctgaaccac atcgcgcgtt gggttgccgc gacgcgagta atcatgcgcg
     3481 cgcggttcat taaatccggt aaagttatag gtgctggaaa gatggatggg agggacaacg
     3541 caaccatact gttcgtcgtc atttaacccg ctacgcactg cgatggtggc ctgtttacgc
     3601 gtcatgtgat gaagttccct gggcttggtc ggtgaaatgt caggcaccag agtaaacact
     3661 gtgttaatgg acgtcaatac atctggacat ctaaacttct ttgcgtatag attgagcaaa
     3721 tcccaaatag ccgttaaaat tatatgcatt atcacgccga caggtgcatt acacgatgtc
     3781 acggtaacgc ctgtacggta aactatgcgg gtttacggtc agtacccaca taaattgtgt
     3841 ggtctggtct caatttattg acgaagagga ttaagtatct catggctgaa tggagcggcg
     3901 aatatatcag cccatacgct gagcacggca agaagagtga acaagtcaaa aagattacgg
     3961 tttccattcc tcttaaggtg ttaaaaatcc tcaccgatga acgcacgcgt cgtcaggtga
     4021 acaacctgcg tcacgctacc aacagcgagc tgctgtgcga agcgtttctg catgccttta
     4081 ccgggcaacc tttgccggat gatgccgatc tgcgtaaaga gcgcagcgac gaaatcccgg
     4141 aagcggcaaa agagatcatg cgtgagatgg ggattaaccc ggagacgtgg gaatactaaa
     4201 gcggaaaaga gcggcgcgga gttaaccagc cgcttttttc atacaaggac gtttaacgat
     4261 gaaaatcagg ctactgatcc tcagcctgct ggtttctgtg cctgcctttg cctggcagcc
     4321 gcaaactggc gacatcatct ttcaaatctc tcgctcatcg caaagtaaag cgatccaact
     4381 ggcgacccat tccgattaca gccacaccgg tatgctggtg atgcgtaaca aaaagcccta
     4441 cgtttttgaa gcggtcggcc cggtgaaata caccccgctc aagcagtgga ttgcccatgg
     4501 tgaaaagggc aaatacgttg ttcgccgcgt tgaaggcgga ctgagtgttg aacaacagca
     4561 aaaactggcg caaacggcga aacgttatct cggcaaaccg tacgatttca gcttctcatg
     4621 gagcgacgat cgccagtact gttcggaagt ggtgtggaag gtttatcaga atgcgctggg
     4681 aatgcgtgtg ggcgaacagc agaagttgaa agagtttgat ctcagcaacc cgctggtcca
     4741 ggcgaagctc aaagaacgct acggcaaaaa tattccgctt gaggaaacgg tggtctcacc
     4801 acaagccgtt ttcgatgcgc cacaactcac cacggttgcc aaagaatggc cgctgttttc
     4861 gtggtaatgg atgatgaaaa aaataattcc cgccgtagtc tgtttgttgc tttgtagttg
     4921 cacgaccttt ccgcaagctg tcgctccggt aaacggtgat gcctccaatt agttgaatta
     4981 aggatataat actcgctttt aaggatgttt catggtcaca gttaaccttc attgtccccg
     5041 ttgccagtcc gttcaggttt accgtcgcgg gcagaacccc aaaagacatg atcgattccg
     5101 cggccgtgac tgtcaccggg tatttcagct cagttactgt tacgagcccc gtaaaccggg
     5161 agttaaagac caaatcgctg aaatggcttt caacggtgcc ggggttcgct atactgcccg
     5221 tacgctgaaa atcggcatta atacagtcat ccgaacttta aaaaactctc gccacgaaga
     5281 ataacgtcat ccccggtcgc tcatgctgat gtggcactca tctgcaaact tagtcagcaa
     5341 tggagctttg tcggcagtaa agcgcgacag cactggctct ggtacgtgta caacacgaaa
     5401 actggtggtg ttctggccta cacttttggc ccccggactg atgaaacctg tcgtgaattg
     5461 ctggcgctgc ttaccctttt accatcggca tgttgaccac tgacgaatgg ggtagttacg
     5521 ctagagagtt accaaaggag aagcatctga ctggtaagat ttttactcag cacattgagc
     5581 gtaacaatct gaccctgaga acccgaatca aacgtctggc ccgcagaacc atctgtttct
     5641 cgagttctgt agaacttcat gaaaaagtca tcggtgcttt tatcgaaaat atatgttcta
     5701 ctaattggag tcatcaccct tctgatgagc tttccttgga tgattaccag actgaata
//
LOCUS       DBFIFI010000247         5750 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.247, whole genome shotgun sequence.
ACCESSION   DBFIFI010000247 DBFIFI010000000
VERSION     DBFIFI010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5750
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.247"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            38..1021
                     /locus_tag="N7B39_004346"
     CDS             38..1021
                     /locus_tag="N7B39_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU2424202.1"
                     /translation="MNKRFVINMVSSLLLGAALISAPLQAAEKVVVNISKVDGMPWFN
                     RMGEGVVEAGKAFGVNASQVGPSSTDAPQQVKIIEDLIARKVNAITIVPNDANVLEPV
                     FKKARDAGIVVLTNESPGQPSANWDIEIIDNEKFAAEYVEHMAKRMGGKGGYVIYVGS
                     LTVPQHNLWADLLVKYQKEHYPDMHEVTRRMPVAESVDVSRRTTLDLMKTYPDLKAVV
                     SFGSNGPIGAGRAVKEKRAKNKVAVYGMMIPSQAASLIKSGDITEGITYDPASAGYAL
                     AAVASTLLKGEEIKPGLEMQNLGKADVDMDKRIIRFHKVLLVNKDNIDSLY"
     gene            1070..2554
                     /locus_tag="N7B39_004347"
     CDS             1070..2554
                     /locus_tag="N7B39_004347"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HGU2424203.1"
                     /translation="METFLSLRHINKTFHATRALRDVSLDFMSGEVHCLAGQNGCGKS
                     TLIKIMSGVYRPDEGAEITLGGKNWSKLTPAASVAQGIQVIYQDLSLFPNLSVWENIA
                     VNHYHHGLFVNRRRLREVAQAAMTSINVTLPLDTLVSELSIARCQLVAICRALAQDAR
                     LIVMDEPTASLTHQEVQGLLQVVHQLRERGICVVFVSHRLEEVMEVSDRISVLKDGEL
                     VGTFPAAEMTTKQLGFLMTGQEFEYQVRELWQGKSSTPVLEVRNLSRHGEYLNINLRV
                     EAGEVVSIVGLLGAGRTELCLSLFGMTRPDAGEILINGQLVTLHSNQDAIRHGIGYVS
                     EDRMSRGLVMAQSIEDNIISTVFHKVKDRFGFLSETKVRDLVDRLIKALTIKVSDPHL
                     PVNTLSGGNAQRVSIAKWLAIGPRLLILDSPTVGVDIANKAGIYGIISDLAAHGIAVL
                     MICDEIEEAWYQSHRILVMQKGQITHSFLPDSSSQARIAEVVNG"
     gene            2547..3518
                     /locus_tag="N7B39_004348"
     CDS             2547..3518
                     /locus_tag="N7B39_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU2424204.1"
                     /translation="MAELKKRHEFWLALLIVVLFVGLAWRSDEFLTFGNLYDLANNYA
                     MLTILACGLFVVLISGGIDISFPAMTIIAQYGMVLLLQKIGGNFAVAFALAGCIGILL
                     GLINALLVNRLRVPSIIITISTLNIFYGLLLWLSKGVWLYDFPPWFEQGVMLFKYTDA
                     DGYDYGLGLPLIAMITVVLLTAFIMNFTSVGRKIYALGGNRESASRIGFSVLKLQLFV
                     YGYMGLMSGAAGVVQSWTVMTVAPDSLLGYELTVLAAVVLGGTSLLGGRGTLTGTLLG
                     VVLLAVMQNGLNLLGVSSYWQTLITGIIIVASISATAWSQHQNRSLL"
     gene            3515..4471
                     /locus_tag="N7B39_004349"
     CDS             3515..4471
                     /locus_tag="N7B39_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU2424205.1"
                     /translation="MKKSWRNNVEFYLIGLLVLTVAAFSITMPEIFWSISNFQSVASQ
                     MPVLGILALAMAVTMLCGGINLSIIATANACSLVMAWVATQYPPGIATVVATLLAGAG
                     AAVIIGLCNGVLIAGIRVLPILATLGMMTLLKGINILVTGGSAIANYPSWVLWLNHAQ
                     WFGIPLPMWLFAAVALGLWILLEKTPLGKAIYLIGSNERATLYSGINTRRVLIWVYVI
                     SALLCAVAAFLMMSKLNSAKASYGESYLLVSILAAVLGGVNPDGGSGRIIGMVLALFL
                     LQIIESGFNILGISPYLTMALWGTLLLCFIQARGMLGLDRVV"
     gene            4558..5607
                     /gene="yahK"
                     /locus_tag="N7B39_004350"
     CDS             4558..5607
                     /gene="yahK"
                     /locus_tag="N7B39_004350"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HGU2424206.1"
                     /translation="MKIKAVGSYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
BASE COUNT         1316 a         1280 c         1616 g         1538 t
ORIGIN      
        1 gaacacttcc tgactaccct gcaatgaggc tgaaatgatg aataaacgtt ttgttatcaa
       61 tatggtgtct tcgctgttat taggcgctgc gctaatttct gcgccgctcc aggcagcgga
      121 aaaagtcgtg gtgaatattt ctaaagtcga tggtatgccg tggtttaatc gcatgggcga
      181 aggtgtggtt gaggcgggta aagcctttgg tgttaatgcc tcacaggtgg ggccttcaag
      241 tactgatgct ccgcaacaag ttaaaattat tgaggattta atcgcccgta aggttaatgc
      301 aattactatc gtgccaaacg atgccaacgt gctggaaccg gtattcaaaa aagcgcgaga
      361 tgccggaatt gtggttctga ccaacgaatc ccccggtcag ccaagtgcta actgggatat
      421 cgaaatcatt gataacgaaa aatttgccgc tgaatatgtt gagcatatgg cgaaacgcat
      481 gggtggcaag ggcggctatg tgatttacgt gggtagtttg accgtgccgc agcataactt
      541 atgggctgac ttgctggtga aataccagaa agagcattac ccggatatgc atgaagttac
      601 ccgccgcatg ccggttgccg agagtgtgga tgtctcacgt cgtactacac tggacctgat
      661 gaaaacctat cctgacttga aagcggttgt ctcctttggt tcaaacggtc cgattggcgc
      721 gggacgtgcg gtgaaagaga aacgggcgaa aaataaagtc gcagtctatg gcatgatgat
      781 cccttcacag gcggcatcac tgattaaaag tggtgatatc accgaaggga ttacttacga
      841 tccggcctct gcaggttacg cactcgcagc cgtcgccagc acgttgctga agggtgaaga
      901 gatcaagcct ggattagaaa tgcagaatct gggtaaggcc gacgtagata tggataagcg
      961 aattatccgt ttccataagg tattactggt caacaaagac aatatcgact cactttatta
     1021 atccatttct tcggggcgaa agccccgatt cttattgcca ggggatgtta tggaaacctt
     1081 cctttccctt cgtcatatca acaaaacgtt tcatgccacg cgagcgctgc gcgatgtttc
     1141 tctcgacttt atgtcgggtg aagtgcactg ccttgccggg caaaacggct gtggtaaatc
     1201 gacgttgatt aaaattatgt ccggtgttta tcgcccggat gagggagcgg aaattacgct
     1261 tggtgggaaa aactggtcaa agctgacacc cgccgcttcg gtggcgcagg ggattcaggt
     1321 gatttatcag gacctctctt tatttcctaa cctgagtgtc tgggagaata tcgccgtgaa
     1381 tcactatcac cacggtctgt ttgttaaccg ccgtcgtctg cgtgaggtgg cgcaggcggc
     1441 aatgactagt attaacgtca cattgccact ggatacgctc gtttctgagc tttcaattgc
     1501 ccgctgtcag ctggtggcaa tttgccgtgc actggcgcag gacgcacggt tgatcgtgat
     1561 ggatgaacca accgcttcgc tgacccatca ggaagtgcaa gggttattac aggtggtgca
     1621 tcaattgcgt gaacgcggga tctgcgtggt ctttgtcagt catcgtctgg aagaagtgat
     1681 ggaagtttcc gaccgtattt cagtgctgaa agatggtgag ctggtcggga cttttccggc
     1741 tgcagagatg accacaaaac aactcggttt cctgatgacc ggccaggagt ttgaatatca
     1801 ggtgcgagag ttgtggcagg gaaaatccag caccccggtg ctggaggtgc gtaatttaag
     1861 tcgtcatggg gaatatctaa atattaactt gcgggtggaa gcgggcgaag tggtatcgat
     1921 tgttggcctg cttggtgcgg ggcgcacgga attatgtctg agtctgtttg gtatgacccg
     1981 accagatgct ggcgagatcc tcatcaacgg ccagctggtt acactgcata gtaaccagga
     2041 cgccatccgc cacggtattg gttatgtttc ggaagatcgc atgtcgcgcg gtttggtgat
     2101 ggcgcaatct attgaagaca acatcattag cactgttttt cacaaggtaa aagatcgctt
     2161 cggctttctg agtgaaacta aagtgcgtga cctggtggac aggctaatca aggcattgac
     2221 catcaaagtc tctgatcctc atcttccggt gaatacgctt tctggtggta acgcgcagcg
     2281 agtatcgatc gccaaatggc tggcgattgg gccacgactg ttgattctcg atagcccgac
     2341 agtcggtgtt gatatcgcca acaaagcagg aatatacggc attatcagcg atctggcggc
     2401 tcatggcatt gcggtattga tgatctgcga tgaaattgaa gaagcgtggt atcaaagcca
     2461 ccggattttg gtaatgcaaa aaggtcagat cacccatagc ttcctgcctg acagcagttc
     2521 tcaggccagg attgcggagg tggtaaatgg ctgaactgaa aaaacgccat gaattctggc
     2581 tggcattact gattgtggtg ctgtttgttg gccttgcctg gcgcagcgac gagtttctga
     2641 cattcggtaa tttgtacgat ctcgccaata actatgccat gttgactatt ctcgcctgtg
     2701 gtttgtttgt ggtgttgatt tccggtggaa ttgatatttc gtttccagca atgaccatca
     2761 ttgcgcaata cggcatggtg ctgttgctgc aaaaaattgg tggcaacttc gctgtcgcgt
     2821 ttgcactggc aggttgcatc ggcattttac ttggcttaat taacgcctta ctggttaatc
     2881 gcctacgggt gccttctatc atcatcacta tctcgacgct gaatattttc tatggcctgc
     2941 tgttatggtt gagtaaaggt gtgtggctgt acgactttcc gccgtggttt gagcaggggg
     3001 ttatgttgtt caagtacacc gatgctgatg gctatgacta tggccttggt ctgccgctga
     3061 tcgccatgat tacggtggtg ctgctgacag cgtttatcat gaatttcacc agtgtagggc
     3121 gcaaaattta tgcccttggc gggaaccgcg aatcagccag tcgcatcggc tttagcgtgc
     3181 tgaaactgca acttttcgtc tacggctata tgggattgat gtctggcgct gcgggtgtag
     3241 tgcagtcgtg gacggtgatg actgtcgccc ccgattctct tctgggttat gagctgacag
     3301 tactggctgc ggtggtgctt ggcggcacta gtttgctcgg cgggcgcggc acgttaactg
     3361 gtactttgct cggcgtggtg ttgttggcag tgatgcaaaa cgggctaaat ttattgggag
     3421 tctcgtctta ctggcaaaca ttgatcaccg gcatcatcat cgttgccagc attagcgcca
     3481 cggcgtggag tcagcatcag aaccggagcc tgctatgaaa aaatcgtggc gtaataacgt
     3541 tgagttttac ttgatcggcc tgctggtgct aactgttgcc gcgttcagca ttactatgcc
     3601 ggagattttc tggtcgataa gtaatttcca gtccgtcgct tcgcaaatgc ccgtgctggg
     3661 cattctggcg ttggctatgg cggtgaccat gctttgcggt ggcattaact tgtcaattat
     3721 agccactgct aacgcctgtt cactggtgat ggcgtgggtg gcgactcagt atccaccggg
     3781 tattgctacg gttgtcgcta cgttactggc gggcgctggc gcggcggtga taatcggttt
     3841 atgtaacggc gtattgattg ctggtattcg agtgttgcca attcttgcaa cgcttgggat
     3901 gatgacgttg ctcaaaggga taaatattct ggtgacaggt ggtagcgcca ttgccaatta
     3961 tccctcatgg gtgctgtggt tgaatcatgc gcaatggttc ggtattccgc taccgatgtg
     4021 gttgtttgcc gcagtggcac tggggctgtg gatattgctg gagaaaacgc cgctcggtaa
     4081 agccatttac ctgattgggt ctaacgaacg agcaacactt tactccggca ttaacactcg
     4141 tcgggtgctg atttgggttt atgtcatttc cgccttgctt tgtgcggtag cggcattttt
     4201 gatgatgtcg aaactcaact cggctaaggc ctcttatggc gaatcgtact tgctggtgtc
     4261 aatcctcgcg gcagtactcg gcggggttaa tccggacggc ggcagcgggc ggataattgg
     4321 tatggtgctg gcgctgtttc ttctgcaaat tattgaaagc ggcttcaata ttctcggtat
     4381 aagcccctac ctgacgatgg ccttatgggg aacgttgctg ctctgtttca ttcaggctcg
     4441 cggcatgttg gggctggatc gggtggttta aatattggct atattcaatg gacgcgtttt
     4501 gccgcgatga catatcaggc gtcgccaaat acacatagct aatcaggagt aaacacaatg
     4561 aagatcaaag ctgttggttc atattccgct aaacaaccac ttgaaccgat ggatatcacc
     4621 cggcgtgaac cgggaccgaa tgatgtcaaa atcgaaatcg cttactgtgg cgtctgccat
     4681 tccgatctcc accaggtccg ttccgagtgg gcggggacgg tttacccctg cgtgccgggt
     4741 catgaaattg tggggcgtgt ggtagccgtt ggtgatcagg tagaaaaaca tgcgccgggc
     4801 gatttggtcg gtgtcggctg cattgtcgac agttgtaaac attgcgaaga gtgtgaagac
     4861 gggctggaaa actactgtga tcacatgacc ggcacctata actcgccgac gccggacgaa
     4921 ccgggccata ctctgggcgg ctactcacaa cagatcgtcg ttcatgagcg atatgttctg
     4981 cgtattcgtc acccgcaaga gcagctggcg gcggtggctc ctttgttgtg tgcagggatc
     5041 accacgtatt cgccgctacg tcactggcag gccgggccgg gtaaaaaagt gggcgtggtc
     5101 ggcatcggcg gtctgggaca tatggggatt aagctggccc acgcgatggg ggcacatgtg
     5161 gtggcattta ccacttctga ggcaaaacgc gaagcggcaa aagccctggg ggccgatgaa
     5221 gttgttaact cacgcaatgc cgatgagatg gcggctcatc tgaagagttt cgatttcatt
     5281 ttgaatacag tagctgcgcc acataatctc gacgatttta ccaccttgct gaagcgtgat
     5341 ggcaccatga cgctggttgg tgcgcctgcg acaccgcata aatcgccgga agttttcaac
     5401 ctgatcatga aacgccgtgc gatagccggt tctatgattg gcggcattcc agaaactcag
     5461 gagatgctcg atttttgcgc cgaacatggc atcgtggctg atatagagat gattcgggcc
     5521 gatcaaatta atgaagccta tgagcgaatg ctgcgtggtg atgtgaaata tcgttttgtt
     5581 atcgataatc gcacactaac agactgaaaa aattaataaa tacgctgtgg tttaacatat
     5641 taacttcgct ctccacttaa ctttttagtt aaggagagcg aaataatatc aaagtagcag
     5701 taaaacctat aacgtaaatt caaattgtta aattaactcc ctccagtacc
//
LOCUS       DBFIFI010000248         5731 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.248, whole genome shotgun sequence.
ACCESSION   DBFIFI010000248 DBFIFI010000000
VERSION     DBFIFI010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.248"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            38..928
                     /gene="prpB"
                     /locus_tag="N7B39_004351"
     CDS             38..928
                     /gene="prpB"
                     /locus_tag="N7B39_004351"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HGU2424207.1"
                     /translation="MSLHSPGKAFRAALTKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYHYEEKLDDLFVRSQAK"
     gene            1084..2253
                     /gene="prpC"
                     /locus_tag="N7B39_004352"
     CDS             1084..2253
                     /gene="prpC"
                     /locus_tag="N7B39_004352"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HGU2424208.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVESKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            2287..3738
                     /gene="prpD"
                     /locus_tag="N7B39_004353"
     CDS             2287..3738
                     /gene="prpD"
                     /locus_tag="N7B39_004353"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HGU2424209.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTGMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVSIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPVFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRARLEQMPVNEYLDLYVI"
     gene            3778..5664
                     /gene="prpE"
                     /locus_tag="N7B39_004354"
     CDS             3778..5664
                     /gene="prpE"
                     /locus_tag="N7B39_004354"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HGU2424210.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLNQIRQ
                     AMEE"
BASE COUNT         1275 a         1563 c         1676 g         1217 t
ORIGIN      
        1 tcaacgaaaa taacaagttg ataacaaagg atgggctatg tctctacact ctccaggtaa
       61 agcgtttcgc gctgcactga ctaaagaaaa tccattgcag attgttggca ccattaacgc
      121 caaccatgcg ctgttggcgc agcgtgccgg atatcaggca atttatctct ccggcggtgg
      181 cgtggcggca gggtcgctgg ggctgcccga tctcggtatt tctactcttg atgacgtgtt
      241 gacagatatt cgccgtatca ccgacgtttg ttcgctgccg ctgctggtgg atgcggatat
      301 cggttttggt tcttctgctt ttaacgttgc gcgtacggtg aaatcgatga ttaaagccgg
      361 tgcggcggga ttgcatattg aagatcaggt tggtgcaaaa cgctgtggtc atcgcccgaa
      421 caaagcgatc gtctcgaaag aggagatggt ggatcggatc cgcgcggcgg tggatgcgaa
      481 aaccgatcct gattttgtga tcatggcgcg caccgatgcg ctggcggtag aggggctgga
      541 tgcggcgatc gagcgtgcgc aggcctatgt tgaagcgggt gccgagatgt tgttcccgga
      601 ggcgattacc gaactcgcca tgtaccgcca gtttgccgat gcggtgcagg tgccaatcct
      661 cgccaacatt accgaatttg gcgccacgcc gctgtttacc accgacgaat tacgcagcgc
      721 tcatgtcgcg atggcgctgt acccgctttc agcgtttcgc gccatgaacc gcgccgccga
      781 acatgtctac aacgtcctgc gccaggaagg cacgcagaaa agcgtcatcg acaccatgca
      841 gacccgcaac gagctgtacg aaagcatcaa ctactaccac tacgaagaga agctcgacga
      901 cctgtttgtc cgtagccagg cgaaataaaa aacgcccgtt ggttgtattc gacaaccgat
      961 gcctgatgcg ccgctgacgc gacttatcag gcctacgggg tgaactgtag gtcggataag
     1021 acgcatggcg tcgcatccga caacaatctc gaccctacaa atgataacaa tgacgaggac
     1081 aatatgagcg acacaacgat cctgcaaaac agtacccatg tcattaaacc gaaaaaatct
     1141 gtggcgcttt ctggcgttcc ggcgggcaat acggcgctct gcaccgtggg taaaagtggc
     1201 aatgacttgc attaccgtgg ctacgatatt cttgatctgg cggaacattg cgaatttgaa
     1261 gaagtggcgc atctgctgat ccacggcaaa ctgccgaccc gtgacgaact cgccgcctac
     1321 aaaacgaaac tgaaagccct gcgcggttta ccggctaacg tgcgtaccgt gctggaagcc
     1381 ttaccggcgg cgtcacaccc gatggatgtt atgcgcaccg gcgtttccgc gctcggctgc
     1441 acgctgccag aaaaagaggg gcataccgtc tctggcgcgc gggatattgc cgacaaactg
     1501 ctggcgtcac ttagctcgat tcttctctac tggtatcact acagccacaa cggcgaacgc
     1561 atccagccgg aaaccgatga cgactccatc ggtggtcact tcctgcatct gctgcacggc
     1621 gaaaagccgt cgcaaagctg ggaaaaggcg atgcatattt cgctggtgct gtacgccgaa
     1681 cacgagttta acgcctccac ctttaccagc cgggtgattg cgggcactgg ctctgatatg
     1741 tattccgcga ttattggcgc gattggtgca ctgcgcgggc cgaagcacgg cggggcgaat
     1801 gaagtgtcgc tggagatcca gcaacgctac gaaacgccgg acgaagccga agccgatatc
     1861 cgcaagcggg tggaaagcaa agaagtggtc attggttttg gtcatccggt ttataccatc
     1921 gccgacccgc gccaccaggt gattaaacgt gtggcgaagc agctctcgca ggaaggcggc
     1981 tcgctgaaga tgtacaacat cgccgatcgc ctggaaacgg tgatgtggga gagcaaaaag
     2041 atgttcccca atctcgactg gttctccgct gtttcctaca acatgatggg cgttcccacc
     2101 gagatgttca caccactgtt tgttatcgcc cgcgtcaccg gctgggcggc gcacattatc
     2161 gaacaacgtc aggacaacaa aattatccgt ccttccgcca attatgttgg accggaagac
     2221 cgccagtttg tcgcgctgga taagcgccag taaacctcta cgaataacaa taaggaaacg
     2281 tacccaatgt cagctcaaat caacaacatc cgcccggaat ttgatcgtga aatcgttgat
     2341 atcgtcgatt acgtcatgaa ctacgaaatc agctccaaag tagcctacga caccgcacat
     2401 tactgtctgc tcgacacgct cggctgcggt cttgaagctc tcgaataccc agcctgtaaa
     2461 aaactgctgg ggccaattgt ccccggcacc gtcgtaccta acggcgtgcg cgtccccgga
     2521 actcagttcc agctcgaccc cgtccaggcg gcatttaaca tcggcgcgat gatccgctgg
     2581 ctcgatttca acgatacctg gcttgcggca gagtggggcc atccttccga caatctcggc
     2641 ggcattctgg caacggcgga ctggctttca cgcaacgcgg tcgccagcgg caaagcgccg
     2701 ttgaccatga aacaggtgct gaccggaatg attaaagccc atgaaattca gggctgcatc
     2761 gcgctggaaa actcctttaa ccgcgtcggc ctcgaccacg ttctgttagt gaaagtggct
     2821 tccaccgccg tggtcgccga aatgcttggc ctgacccgcg aggaaattct caacgctgtt
     2881 tcgctggcgt gggtggacgg tcagtcgctg cgcacctatc gccatgcgcc gaacaccggc
     2941 acgcgtaaat cctgggcggc gggcgatgcc acttcccgcg cggtacgtct ggcactgatg
     3001 gcgaaaacgg gcgaaatggg ttacccgtca gccctgactg cgcctgtgtg gggcttctac
     3061 gacgtctcct ttaaaggtga atcgttccgc ttccagcgtc cgtacggttc ttacgtgatg
     3121 gaaaatgtgc tgttcaaaat ctccttcccg gcggagttcc actcccagac ggcagttgaa
     3181 gcagcgatga cgctctatga acagatgcag gcagcaggca aaacggcggc agatatcgaa
     3241 aaagtgtcca tccgcaccca cgaagcctgt attcgcatca tcgacaaaaa ggggccgctc
     3301 aataacccgg cagaccgcga ccactgcatt cagtacatgg tggcgatccc actgctattc
     3361 gggcgcttaa cggcggcaga ttacgaggac aacgttgcgc aagataaacg cattgacgcc
     3421 ctgcgcgaga agatcaattg ctttgaagat ccggtattta ccgctgacta ccacgacccg
     3481 gaaaaacgcg ccatcgccaa tgccattacc cttgagttca ccgacggcac acgatttgaa
     3541 gaagtggtgg tggagtaccc cattggtcat gctcgccgcc gtcaggatgg tattccgaaa
     3601 ctggtcgata aattcaaaat caatctcgcg cgccagttcc cgactcgcca acagcagcgc
     3661 attctggagg tttctctcga cagagctcgc ctggaacaga tgccggtcaa tgagtatctc
     3721 gacctgtacg tcatttaagt aaacggcggt aaggcgtaag ttcaacagga gagcattatg
     3781 tcttttagcg aattttatca gcgttcgatt aacgaaccgg agcagttctg ggccgagcag
     3841 gcccggcgta ttgactggca gacgcccttt acgcaaacgc tcgatcacag caatccgccg
     3901 tttgcccgtt ggttttgtga aggccgaacc aacttgtgcc acaacgctat cgaccgctgg
     3961 ctggagaaac aaccagaggc gctggcgctg attgccgtct cttcggaaac agaagaagag
     4021 cgcaccttta cctttcgcca gcttcatgac gaagtaaacg cggtggcgtc aatgctgcgc
     4081 tcgctgggcg tgcagcgtgg cgatcgggtg ctggtgtata tgccgatgat tgccgaagcg
     4141 catattactc tgctggcctg cgcgcgcatt ggcgctattc actcggtggt gtttggggga
     4201 tttgcctcgc acagcgtggc ggcgcgaatt gatgatgcta aaccggtgct gattgtctcg
     4261 gctgatgccg gagcgcgcgg tggcaaaatc attccctata aaaaattgct cgacgatgcg
     4321 ataagtcagg cgcagcacca gccacgccat gttttgctgg tggatcgcgg gctggcgaaa
     4381 atggcacgcg tcagcgggcg ggatgtcgat ttcgcgtcgt tgcgccatca acacatcggc
     4441 gcgcgggtac cggtggcgtg gctggaatcc aacgaaacct cctgcattct ctacacctcc
     4501 ggcacgaccg gcaaacctaa aggcgtgcag cgtgacgttg gcggatatgc ggtggcgctg
     4561 gcgacctcga tggacaccat ttttggcggc aaagcgggcg gtgtgttctt ttgtgcttcg
     4621 gatatcggct gggtggtggg gcattcgtat atcgtctacg cgccgctgct ggcggggatg
     4681 gcgactatcg tttacgaagg attaccgacc tggccggact gcggcgtgtg gtggaaaatc
     4741 gtcgagaaat atcaggttag ccggatgttc tcagcgccga ccgccattcg cgtgctgaaa
     4801 aaattcccta ccgctgaaat tcgcaaacac gatctctcgt cgctggaagt gctctatctg
     4861 gctggagaac cgctggacga gccgaccgcc agttgggtga gcaatacgct ggatgtgccg
     4921 gtcatcgaca actactggca gaccgaatcc ggctggccga ttatggcgat tgctcgcggt
     4981 ctggacgaca ggccgacgcg tctgggaagc cccggcgtgc cgatgtatgg ctataacgtg
     5041 cagttgctta atgaagtcac cggcgaaccg tgtggcgtca acgagaaagg gatgctggtg
     5101 gtggaagggc cgctgccgcc ggggtgtatt cagaccatct ggggcgacga cgaccgcttt
     5161 gtgaagacgt actggtcgct gttttcccgc ccggtgtacg ccacctttga ctggggcatc
     5221 cgtgacgctg acggttatca ctttattctc gggcgcactg acgatgtgat taacgttgcc
     5281 gggcatcggc tggggacgcg cgagattgaa gagagtatct ccagtcatcc gggcgttgcc
     5341 gaagtggcgg tggttggggt gaaagatgcg ctgaaagggc aggtggcggt ggcgtttgtc
     5401 attccgaaag agagcgacag tctggaagat cgtgatgtgg cgcattcgca agagaaggcg
     5461 attatggcgc tggtggacag ccagattggc aactttggcc gcccggcgca cgtctggttt
     5521 gtctcgcaat tgccaaaaac gcgatccgga aaaatgctgc gccgcacgat ccaggcgatt
     5581 tgcgaaggac gcgatcctgg ggatctgacg accattgatg atcctgcgtc gttgaatcag
     5641 atccgccagg cgatggaaga gtaaggtatt gtcggatgcg tcgcgtggcg catccgacag
     5701 ttgtgcgcag atgcctgatg cgacgctggc g
//
LOCUS       DBFIFI010000249         5597 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.249, whole genome shotgun sequence.
ACCESSION   DBFIFI010000249 DBFIFI010000000
VERSION     DBFIFI010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5597
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.249"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..332
                     /locus_tag="N7B39_004355"
     CDS             <1..332
                     /locus_tag="N7B39_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HGU2424211.1"
                     /translation="ESDKSKIFFCLSAVFANISHSNVFNGIPDASKTLKRYAFALLAK
                     AHSLDESMISNQTFNTYKTVLLDFNNLSNEEANQLRISSLYRDMVRYAQYRFSKVLSE
                     WTPDAWL"
     gene            616..2766
                     /gene="dinG"
                     /locus_tag="N7B39_004356"
     CDS             616..2766
                     /gene="dinG"
                     /locus_tag="N7B39_004356"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HGU2424212.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDMLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            2794..3756
                     /gene="ybiB"
                     /locus_tag="N7B39_004357"
     CDS             2794..3756
                     /gene="ybiB"
                     /locus_tag="N7B39_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HGU2424213.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREEMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WIQRCLAGREPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            3897..4982
                     /gene="hcxB"
                     /locus_tag="N7B39_004358"
     CDS             3897..4982
                     /gene="hcxB"
                     /locus_tag="N7B39_004358"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HGU2424214.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDDFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            5049..5108
                     /gene="ybiE"
                     /locus_tag="N7B39_004359"
     CDS             5049..5108
                     /gene="ybiE"
                     /locus_tag="N7B39_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HGU2424215.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(5211..5471)
                     /gene="ybiJ"
                     /locus_tag="N7B39_004360"
     CDS             complement(5211..5471)
                     /gene="ybiJ"
                     /locus_tag="N7B39_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HGU2424216.1"
                     /translation="MKTINTVVAAMALSALSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
BASE COUNT         1352 a         1368 c         1560 g         1317 t
ORIGIN      
        1 gtgaaagtga taagtctaaa atatttttct gcctgtctgc cgtatttgca aatatttctc
       61 acagtaatgt ctttaacggt attcctgatg cgtcaaaaac tttaaaacgt tatgcatttg
      121 ctttattggc gaaagctcat tctttagatg aaagtatgat aagcaatcaa acttttaata
      181 catacaaaac ggtattgctt gatttcaata atctgtctaa tgaagaggcc aaccagttgc
      241 gcataagttc gttatatcgt gatatggtcc gctatgcaca atatcgtttt tctaaagttt
      301 tatccgagtg gacgccagac gcatggctgt aaagatcgca gcagaataat ggcattgatg
      361 gccctggctc aggccagggt cggcaatacc ggttcctgcg aagcgatgac ggcggcaatt
      421 gtaaccacga aatgcggtat cacttgctgt tttctgccct gaagactggc gctcactgtg
      481 ccgtcgaatt ttcaatcaaa tatattgcga gagggcgtgt ttgcgagccg ctttccagaa
      541 acagaaaaac cattacccct gaaaaccgaa aaatgccaca atattggctg tttatacagt
      601 atttcaggtt ttctcatggc attaaccgcc gcgcttaaag cgcaaattgc cgcctggtat
      661 aaggcgcttc aggaacagat ccccgacttt attccccgtg cgccgcagcg gcagatgatt
      721 gcggacgtcg ccaaaacgct ggccggagaa gaagggcggc atctggcgat tgaagccccc
      781 actggcgttg ggaaaacgct ctcctatttg attcccggca tcgccattgc ccgcgaagag
      841 caaaaaacgc tggtggtgag taccgccaac gtggcattgc aggatcagat ctacagcaaa
      901 gatttgccac tgctgaaaaa gatcattccc gatcttaaat tcactgccgc ttttgggcgt
      961 gggcgctacg tttgcccgcg taatctgacg gcgcttgcca gcactgagcc cacgcaacag
     1021 gatctgctgg cgtttcttga cgacgaactg acgccaaata atcaggaaga gcaaaaacgt
     1081 tgtgcgaagc tgaagggcga tctcgacact tataaatggg atggtctgcg cgatcatacg
     1141 gatatcgcta tagatgacga tctctggcgt cgtttaagta ccgacaaagc cagctgcctc
     1201 aaccgcaact gttactacta tcgcgaatgc ccgttttttg tcgctcgtcg ggaaattcag
     1261 gaagcggaag tggtggtggc aaaccatgcg ctggtgatgg cggcgatgga aagcgaagcc
     1321 gtattgcctg acccgaaaaa tttactgctg gtgctggacg aaggccatca cctgccagat
     1381 gtggcgcggg atgcgctgga gatgagtgcc gaaatcaccg cgccatggta tcggctacag
     1441 ctggacttgt tcacgaaact ggtcgctacc tgcatggagc agtttcgccc gaagaccatc
     1501 ccgccgttgg cgatccctga acgtttgaat gcgcattgtg aagagctgta tgagcttatc
     1561 gcctcattaa acaacattct caatctctac atgcctgccg ggcaggaagc agaacaccgt
     1621 tttgcgatgg gcgaactgcc tgatgaagtg ctggagatct gccagcggct ggcaaaactc
     1681 accgagatgc tgcgtggtct ggcggagtta tttcttaacg atttaagtga gaaaaccggc
     1741 agccatgaca ttgtacgtct gcatcggttg attttgcaga tgaaccgcgc gttggggatg
     1801 ttcgaggcgc aaagcaaact ctggcggctg gcttcgctgg cgcaatcttc cggtgcaccg
     1861 gtgaccaaat gggcgacgcg ggaagagcgc gaagggcagc tacacctctg gtttcactgc
     1921 gtgggaatac gtgtcagcga tcagctggaa aggctgctgt ggcgcagtat tccgcacatt
     1981 attgtcacct ccgcaacctt gcgttcgctg aacagttttt cgcgtttgca ggagatgagc
     2041 gggctgaaag agaaagcggg cgaccgtttt gtggcgctgg attccccctt taaccactgc
     2101 gaacagggca aaattgttat tccccggatg cgcgttgagc cttccatcga caacgaagaa
     2161 cagcatattg ctgaaatggc ggcctttttc cgtgagcagg tggagagcaa aaaacatctc
     2221 ggtatgttgg tgctgtttgc cagcgggcgt gcgatgcagc gctttctcga ctatgtgacg
     2281 gatttacgtc tgatgttgct ggtgcagggc gatcagccgc gttaccgttt agttgaactg
     2341 caccgcaaac gcgtcgccaa cggtgagcgt agtgtgctgg tgggcttaca gtcatttgcc
     2401 gaagggcttg atttgaaagg tgatatgctc agccaggtgc atatccacaa aatcgctttt
     2461 ccgcctatcg acagcccggt ggtgatcact gaaggggaat ggctgaaaag cctcaaccgc
     2521 tatccatttg aggtgcaaag cctgccgagc gcctcgttta acctgattca gcaggttggg
     2581 cgactgattc gaagtcacgg ttgctggggc gaagtggtga tttacgataa acgcttgctg
     2641 accaaaaatt atggcaagcg actactggat gcattaccgg tatttccgat agagcaaccg
     2701 gaagtccctg aaggtatagt taaaaagaaa gaaaaaacga aatccccacg ccgtcggcgg
     2761 cgttaatgat gtgagtcagg taaggagtcg taaatggact atcgcaaaat cattaaagag
     2821 atcgggcgcg ggaaaaacca cgcgcgcgat ttagaccggg atactgcgcg cggtctgtat
     2881 gcccatatgc tcaatggtga agtccctgac cttgagttgg gcggtgtatt gattgcgctg
     2941 cgtatcaaag gggaagggga agcagagatg ctcggctttt acgaagccat gcaaaatcac
     3001 accatcaagc tgacgccgcc tgcaggcaag ccgatgccga ttgtcatccc cagttacaac
     3061 ggcgcgcgta aacaggccaa cctgacgccg ttgttggcga ttctcctgca caaactcggt
     3121 tttcctgtgg tggttcacgg ggttagcgaa gatccaactc gcgtgctgac cgaaaccatt
     3181 ttcgaactga tgggtattac gccaacgttg cacggcgggc aggcgcaggc gaagctcgac
     3241 gaacatcaac cggtgtttat gcctgtcggc gcgttttgcc cgccgctgga aaaacaactg
     3301 gcgatgcgct ggcggatggg cgtgcgtaac agtgcacata ccctggcgaa actggcgacg
     3361 ccatttgccg aaggtgaggc actgcgtctt tccagcgttt cgcatccgga atacatcggg
     3421 cgagtcgcga agttctttag cgatatcggc gggcgggcgt tactaatgca tggtactgaa
     3481 ggcgaagtgt atgctaatcc gcagcgctgc ccacagatca atctcattga tcgtgaagag
     3541 atgcgggtgc tgtatgaaaa acaggaccct gctggaagcg agttactgcc acaagcaaaa
     3601 gatccggaaa ccacggcgca gtggattcag cgttgccttg ctggccgcga accgattccc
     3661 gaatcgctga aaatccagat ggcttgctgc ctggtggcta cgggtgaagc ggcaactatc
     3721 agcgatggcc tggcgcgcgt taatcaggca ttttaattct tttctcagcc ggatgagcca
     3781 tgtcatccgg cttttacccc accgcaatat gaaattcctg catctttatt gaccttcccg
     3841 cgcccggcgt gcagcataaa aaaacaacaa acacataaca taaacaggag ttaaccatgg
     3901 aaagtggtca tcgctttgat gctcagacgc tgcacagctt tattcaggct gtatttcgtc
     3961 agatgggtag cgaggaacaa gaagcgaaat tagttgccga tcatttaatc gcggcaaacc
     4021 tggcagggca tgattcacat ggtattggca tgatcccaag ctatgtacgc tcctggagtc
     4081 aggggcacct gcaaattaac catcatgcca aaatcgttaa agaggcgggg gcggcggtca
     4141 cgctcgatgg cgatcgcgca tttggtcagg tcgcggcaca tgaagcgatg gcgctgggga
     4201 ttgagaaagc gcatcagcac ggtattgctg ccgtggcgct acataactcg catcatatcg
     4261 gccgtatcgg ttactgggcg gagcagtgtg cagcggcggg gtttgtctct atccactttg
     4321 ttagcgtggt cggtattcca atggtcgcac cgttccacgg tcgcgacagc cgctttggca
     4381 cgaatccgtt ctgtgtggtt ttcccacgta aagatgattt tccgctgttg cttgattacg
     4441 ccaccagcgc cattgcattt ggcaaaaccc gcgtcgcctg gcataaaggc gtccccgtgc
     4501 cgccaggttg cctgattgac gttaacggcg tgccgacgac caatccggcg gtaatgcagg
     4561 agtcgccgtt gggttcgctg ttgacctttg ccgaacataa aggctacgcc ctggcggcaa
     4621 tgtgtgaaat tcttggcggg gcgctttctg gcggtaaaac gacgcatcag gaaacgttac
     4681 aaaccagtcc cgatgccatt cttaactgca tgaccactat catcatcaac ccggaactgt
     4741 tcggcgcgcc ggattgtagc gcgcagaccg aagcctttgc cgagtgggtg aaagcctcgc
     4801 cgcatgatga cgataagccg attttgctcc cgggcgagtg ggaagtgaac acgcgtcgcg
     4861 aacggcagga gcaggggatt ccattggatg cgggaagctg gcaggccatt tgtgatgcgg
     4921 cgcggcagat tggtatgccg gaagagacgt tgcaggcttt ctgtcagcag ttagccagct
     4981 aaaaaaaagc ccgtccagtg gcggacgggc aaacaagggt aacataggat caatgagggt
     5041 tagagcatat gcgtctgtcg gcaaacagac agggaaatac ttgtgcagga cgtagcgtaa
     5101 acgcctgatc cgtccaacgg ttcggatttt gtaggcctga taagacgcgg tagcgtcgca
     5161 tcaggcatct gttgccggat gcggcgtaaa cgccttatcc gtcctacgaa ttacttataa
     5221 attaccgcag taccgcttaa tttattgttg ttggtggcgg aagtgatgct atatccactg
     5281 gcaccggctg ccgccgcttt ctcagccagt ttcgcttcca gggcatcgag ggtggatgcg
     5341 ccatcggcag aaaccacgcc gattttattc atgttctgtg cctgggatgc cgttaccggt
     5401 tccgcggcaa acacgccaaa tgacagagct gaaagagcca tagcagcaac aacagtattg
     5461 atagttttca taattaatct ctcgcaggtg atttttgtat aagtgacgtt gtttcgtcga
     5521 tgtgatgagt atcacgtttt tttacgagag ataaaatcga agagaattga ccgccttgtt
     5581 caaataaatt gattgat
//
LOCUS       DBFIFI010000250         5554 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.250, whole genome shotgun sequence.
ACCESSION   DBFIFI010000250 DBFIFI010000000
VERSION     DBFIFI010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5554
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.250"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..485
                     /locus_tag="N7B39_004361"
     CDS             <1..485
                     /locus_tag="N7B39_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HGU2424217.1"
                     /translation="TNVPGSDDLNGINVKYRYEFTDTLGLVTSFSYAGDKNRQLTRYS
                     DTRWHEDSVRNRWFSVMAGPSVRVNEWFSAYAMAGMAYSRVSTFSGDYLRVTDNKGKT
                     HDVLTGSDDGRHSNTSLAWGAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYKF
                     "
     gene            550..3465
                     /locus_tag="N7B39_004362"
     CDS             550..3465
                     /locus_tag="N7B39_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HGU2424218.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMTEDDVRPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDASTSAREAATHATDAADSARAASTSAG
                     QAASSAQSASSSAGTASTKATEASKSAAAAESSKSAAATSAGAAKTSETNAAVSQQSA
                     ATSASTATTKASEAASSARDASASKEAAKSSETSAASSASSAASSATAAGNSAKAAKT
                     SETNAKSSETAAEQSASAAAGSKTAAALSASAASTSAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQASAAASSASAAKTSETNAKASETSAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKSSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSESLAATPKAVKAAYELANGKYTAQDATTA
                     QKGIVQLSNATNSTSEMLAATPKSVKAAYDLANGKYTAQDATTAQKGIVQLSSATNSA
                     SETLAATPKAVKAANDNANGRVPSARKVNGKALSSDITLTPKDIGTLNSTTMSFSGGA
                     GWFKLATVTMPQASSVVSITLIGGAGFNVGSPQQAGISELVLRAGNGNPKGITGALWQ
                     RTSTGFTNFAWVNTSGDTYDIYVAIGNYATGVNIQWDYTSNASVTIHTSPAYSANKPE
                     GLTDGTVYSLYTPSEQFYPPGAPIPWPSDTVPSGYALMQGQTFDKSAYPKLAAAYPSG
                     VIPDMRGWTIKGKPASGRAVLSQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTKSTN
                     NTGAHTHSVSGTAASAGNHTHSVTGASAVSQWSQNGSVHKVVSAASVNTSAAGAHTHS
                     VSGTAASAGAHAHTVGIGAHTHSVAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            3465..4046
                     /locus_tag="N7B39_004363"
     CDS             3465..4046
                     /locus_tag="N7B39_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HGU2424219.1"
                     /translation="MAFRMSEQPRTIKIYNLLTGTNEFIGEGDAYIPPHTGLPANSTY
                     IAPPDIPAGFVAVFNSDESSWHLVEDHRGKTVYDVASGDALFISELGSLPENVTWLSP
                     EGEFQKWNGTAWVKDAEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEEE
                     TSLLEAWKKYRVLLNRVDTSTAPDIEWPVIPEV"
     gene            complement(4166..5056)
                     /locus_tag="N7B39_004364"
     CDS             complement(4166..5056)
                     /locus_tag="N7B39_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HGU2424220.1"
                     /translation="MFRHVKKLQYTVRVSEPNPGLANLLLEQFGGPQGELAAACRYFT
                     QGLSDEDAGRRDMLMDIATEELSHLEIIGSLVGMLNKGAKGALAEGTENEAELYRSLT
                     QNGNDSHITSLLYGGGPALTNSAGVPWTAAYVDTIGEVTADLRSNIAAEARAKIIYER
                     LINVTDDPGVKDTLAFLMTREAAHMLSFEKALHSIRNTFPPGKLPPLEKYKNVYYNMS
                     EGEDVRGSWNSDENFDYVSDPVSAVDGGDGKASINLSTKQEAMIKAMATRLKSHEDIN
                     PVTGAELAEGEPQTKINSKN"
     gene            complement(5075..>5554)
                     /locus_tag="N7B39_004365"
     CDS             complement(5075..>5554)
                     /locus_tag="N7B39_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HGU2424221.1"
                     /translation="WLRDAHAMEKQAEKMLESMASRIENYPELRSRIEQHISETKNQL
                     SQLESILDRNNISRSVIKDSMSKMAAFGQSIGGIFPSDEIVKGSISGYVFEQFEIACY
                     TSLLAAAKNAGDTASVPIIEAILNEEKQMAEWLLNHIPDTTEQFMVRSEIDGVEAKK"
BASE COUNT         1471 a         1345 c         1447 g         1291 t
ORIGIN      
        1 ggaccaacgt tcccggcagt gatgatctga acgggattaa cgtgaaatac cgttatgagt
       61 ttacggatac gctggggctg gtgacgtcat tcagctatgc aggagacaag aatcgccagc
      121 tgacccgtta cagcgatacc cgctggcatg aagattccgt gcgtaaccgc tggttcagcg
      181 tgatggcggg gccgtctgtg cgcgtgaatg aatggttcag cgcgtatgcg atggcgggta
      241 tggcttacag ccgtgtttcg actttttccg gggattacct tcgcgtaact gacaacaagg
      301 ggaaaacgca cgatgtgctg accggaagtg atgacggtcg ccacagcaac acgtctctgg
      361 cgtggggggc tggcgtgcag tttaacccga ccgaatccgt ggccattgat attgcttatg
      421 aaggttctgg cagtggtgac tggcgcactg acgggttcat cgtgggtgtt ggttataaat
      481 tctgattagc caggtaacac agtgttatga cagcccgccg gttcaggcgg gcttttttgt
      541 ggggtgaata tggcagtaaa gatttcaggt gtactgaaag acggcacagg aaaaccggta
      601 cagaactgca caatccagct gaaagcaaaa cgtaacagta ccacggtggt ggtgaacacg
      661 ctggcctcag aaaatccgga tgaagccggg cgttacagca tggacgttga gtacggtcag
      721 tacagcgtta ttctgttggt ggaagggttc ccgccgtcac atgccgggac catcaccgtg
      781 tatgaagatt ctcaacccgg tacgctgaat gattttcttg gtgccatgac tgaggatgat
      841 gtccgtccgg aggcactgcg ccgttttgag ctgatggtgg aagaggtggc gcgtaacgcg
      901 tccgcggtgg cacagaacac ggcagccgcg aagaagtcag ccagcgatgc cagcacatca
      961 gcccgtgagg cggcaaccca tgcgactgat gctgcggact cagcacgcgc agccagcacg
     1021 tcagccggac aggccgcgtc gtcggctcag tcagcgtctt ccagcgcagg aacggcatca
     1081 acaaaggcta ctgaagcatc aaaaagtgct gccgctgcag agtcctcaaa aagcgcggcg
     1141 gctaccagtg ccggtgcggc gaaaacgtca gaaacgaatg cggcagtgtc acaacaatca
     1201 gccgccactt ctgcatccac cgcgaccacg aaagcgtcag aagctgcctc ctcagccagg
     1261 gatgcgtcgg cttcaaaaga ggcggcaaaa tcatcagaaa cgagcgcagc ctcgagcgcc
     1321 agtagtgcag cctcctcggc aacggcggca ggcaattccg cgaaggcggc caaaacgtct
     1381 gagacaaacg ctaagtcctc tgaaacggca gcagaacaga gtgcctccgc agcagcaggc
     1441 tcaaaaacag cggctgcatt atctgccagt gccgcgtcaa caagtgccgg gcaggcctca
     1501 gccagtgcca ccgccgccgg aaaatcggca gaaagtgccg catcgtctgc ttcaacagcc
     1561 acaacgaagg ctggcgaagc cactgaacag gccagcgcag cagcgagttc tgcttccgca
     1621 gcgaagacat ccgaaacgaa cgcgaaagcg tcggaaacca gcgcagaatc ctcaaaaacg
     1681 gctgccgcat cgtcagccag ttcggcggcg tcatcggcat catctgcgtc tgcttcaaaa
     1741 gatgaggcga ccagacaagc gtcagcagcg aagagcagcg ccacgacggc atccacgaag
     1801 gcgacagagg cagctggtag tgcgacggca gcagctcaga gcaaaagtac ggcggaatct
     1861 gcagcaacgc gcgctgagac agcggcaaaa cgggcagagg atattgcatc cgccgtggcg
     1921 cttgaggatg cgagcacgac gaaaaagggg atagtacagc tcagcagtgc gactaacagc
     1981 acttccgagt cactggcggc aacgccaaaa gccgttaagg ccgcgtatga gctggctaac
     2041 gggaaataca ccgcacagga tgcaacgaca gcacagaaag ggatagttca gcttagcaac
     2101 gcgaccaaca gcacatctga aatgctggcg gcaacgccaa agtcggtaaa ggcagcctat
     2161 gaccttgcta acgggaaata tactgctcag gacgctacga cagcacaaaa aggaattgtc
     2221 cagctcagta gtgcaaccaa cagcgcatct gaaacgcttg ccgcgacacc gaaagcagtg
     2281 aaagcagcta atgataatgc gaatggtcgg gtaccttctg cccgtaaggt gaatggtaag
     2341 gcgctttcat cggatataac actgacgccg aaagatattg gtacgcttaa ctcaacaaca
     2401 atgtcattca gcggtggtgc tggttggttc aaattagcaa cggtaaccat gccacaggcg
     2461 agttctgttg tttcaattac gttgattggt ggcgcgggat ttaacgtggg gtcacctcaa
     2521 caggcaggta tatctgaact tgttttgcgt gcaggtaatg gtaatccgaa ggggattact
     2581 ggtgctttat ggcagcgcac atcgacaggg tttacaaatt ttgcctgggt caatacatct
     2641 ggtgatactt acgatattta cgttgcaatc ggaaattatg cgactggtgt aaatattcaa
     2701 tgggattata ccagtaatgc cagcgtgacg attcatacgt caccagcata ttctgctaat
     2761 aagccggaag ggttaacgga cggtacagtt tattcactct atacgccatc agagcagttt
     2821 tatccgcctg gcgcaccaat cccgtggcca tcagataccg ttccgtctgg ctatgccctg
     2881 atgcaggggc agacttttga caaatctgca tacccgaaac ttgcagccgc ttatccgtca
     2941 ggcgtgatcc ctgatatgcg tggctggacg attaagggca aacctgccag tggtcgggcc
     3001 gtattgtctc aggaacagga cggcattaaa tcgcacaccc acagcgccag cgcatccagt
     3061 acggatttgg ggacgaaaac cacatcgtcg tttgattacg gcactaaatc cacgaataac
     3121 accggggcgc acacgcacag tgtgagcggt acagccgcaa gtgccggaaa ccatactcat
     3181 agtgtcacag gcgcatcagc agtcagccag tggtcacaaa atgggtcagt acataaggta
     3241 gtgtctgcgg ccagtgtgaa tacaagtgct gcaggagcgc acactcatag tgtcagcggc
     3301 acagctgcat ctgcaggtgc tcacgcacat actgtcggta ttggtgctca tacgcactct
     3361 gttgcgattg gctcacatgg acacaccatc accgttaacg ctgcgggtaa cgcggaaaac
     3421 actgtcaaaa acatcgcatt taactacatt gtgaggcttg cataatggca ttcagaatga
     3481 gtgaacaacc acggaccata aaaatttata atctgctgac cggaactaat gaatttattg
     3541 gtgaaggtga cgcatatatt ccgcctcata caggtctgcc agcaaacagt acctatattg
     3601 caccgccaga tattccggct ggcttcgtgg ctgttttcaa cagtgatgag tcatcgtggc
     3661 atctcgttga agatcatcgg ggtaaaacgg tttatgacgt ggcttccggc gacgcgttat
     3721 ttatttctga actcggctca ttaccggaaa atgttacctg gttatcgccg gaaggggagt
     3781 ttcagaagtg gaacggcaca gcctgggtga aagatgcaga agcagaaaaa ctgttccgga
     3841 tccgggaggc ggaagaaaca aaaaacagcc tgatgcaggt agccagtgag catattgcgc
     3901 cacttcagga tgctgtagat ctggaaatcg caacggagga agaaacctca ttgctggaag
     3961 cctggaaaaa gtatcgggtg ttgctgaacc gtgttgatac atcaactgca cctgatattg
     4021 agtggcctgt aatacctgaa gtttaaaata aaagcccgct taaaatatcg cgggcttcga
     4081 aatataaatg tattttcaga ggctagagct taggatatct cagcggcaat taacttatga
     4141 gggatagcta aataccagaa gataattagt ttttactatt tatctttgtt tgtggttctc
     4201 cttcagcaag ctcagcgcca gtgacaggat tgatgtcttc atgggatttc aaccttgttg
     4261 ccatagcttt tatcatagct tcttgcttgg tcgaaagatt tatcgaggcc ttcccatccc
     4321 caccgtcaac cgctgaaact ggatcggaaa catagtcaaa gttttcatca ctgttccaac
     4381 tacctctgac gtcttcacct tctgacatgt tgtaatatac gtttttgtat ttctcaagtg
     4441 gtggtaattt tcctggtgga aaggtgttac gaattgaatg taatgctttt tcaaatgaaa
     4501 gcatgtgtgc tgcttcccgg gtcattaaaa atgccagagt gtcttttact ccaggatcat
     4561 cagtaacatt aatgagacgt tcgtaaatga tctttgcccg agcttcagct gcaatatttg
     4621 aacgaagatc ggccgtgact tcgccaatag tatcaacata agctgcagtc cagggtactc
     4681 cagctgaatt agttaatgct gggcctcctc cgtagaggag agaagttata tggctatcat
     4741 tgccattttg agttaaggaa cggtaaagct cagcttcatt ctcagttcct tcagcaagtg
     4801 ctccttttgc acctttgttg agcataccaa caagagaacc aataatttcg agatgactta
     4861 gttcttctgt tgctatgtcc atcagcatat cccttcggcc tgcatcttca tcacttaagc
     4921 cttgagtgaa gtatcggcat gctgctgcaa gctcaccctg tggcccaccg aattgttcta
     4981 agagtagatt agccaagcct gggtttggct cacttacacg tactgtatat tgaagttttt
     5041 tcacgtgtct aaacataatg cctcctcaaa agaattattt tttggcttca actccatcaa
     5101 tttcagatct gaccatgaat tgctcagtgg tgtcaggaat atggttaaga agccattcag
     5161 ccatttgttt ttcttcatta agaattgctt caataatggg tactgaagct gtatcacccg
     5221 catttttggc tgctgcgaga agagatgtgt aacacgcaat ttcaaattgt tcgaacacat
     5281 agccactgat cgaacctttg acaatttcat cagatggaaa aattccacca atggattgtc
     5341 caaacgcggc cattttactc atagagtctt taatgacaga gcgcgaaata ttattacgat
     5401 caagtatgct ttcaagctgt gaaagctggt tctttgtttc gctaatgtgc tgctcaattc
     5461 tggaacgtaa ctcaggatag ttttctatcc ggctggccat agactcaagc attttttcag
     5521 cttgtttttc catcgcatgt gcatcacgaa gcca
//
LOCUS       DBFIFI010000251         5443 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.251, whole genome shotgun sequence.
ACCESSION   DBFIFI010000251 DBFIFI010000000
VERSION     DBFIFI010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5443
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.251"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..416
                     /locus_tag="N7B39_004366"
     CDS             <1..416
                     /locus_tag="N7B39_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HGU2424222.1"
                     /translation="IVGWRVSSSMETTFVLDALEQALWARRPSGTVHHSDKGSQYVSL
                     AYTERLKEAKLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWKNRAEVELATLTWV
                     DWYNNRRLLGRLGHIPPAEAEKAYYASIRNDDLAA"
     gene            complement(470..688)
                     /gene="ccdA"
                     /locus_tag="N7B39_004367"
     CDS             complement(470..688)
                     /gene="ccdA"
                     /locus_tag="N7B39_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HGU2424223.1"
                     /translation="MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERW
                     KAENQEGMAEVARFIEMNGSFADENRDW"
     gene            1231..1830
                     /locus_tag="N7B39_004368"
     CDS             1231..1830
                     /locus_tag="N7B39_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001495930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGU2424224.1"
                     /translation="MTSLSPDTVRRIEDAAAALIAAGTPNPTNEQVRQHLGGGSLSHI
                     SPVMRAFRARQREQATPLPPELAQLLTGQLGLLWQAAVKQAEAGALAAREQADDDIAR
                     ADKERDEALANVAALESELAVLREVVAERDRLLQEVRELRAEALPLREQVARLTATGE
                     HLAAQLQDTKAELKEAREDGRQLQTELLALARQDGKVKK"
     gene            1827..2447
                     /locus_tag="N7B39_004369"
     CDS             1827..2447
                     /locus_tag="N7B39_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424225.1"
                     /translation="MRQERVLRIKAFRRALELGGGERADGCRHFPRWKTELRNFPHSS
                     CDLASNTLAYYLKERERCHPCIIFMEGNAGFHEAENSTVHGHVIVLLDGEYIDLTLDQ
                     FDEYPDYIPYEPVESDGQIGKLLRNIMKHEDPVKTRQIDLDGGEELYAWLRDTADEVL
                     AADPEWQARERSIEEAREAAITVFPFLSDVQKTECEQGPDSQEAAR"
     gene            2444..2644
                     /locus_tag="N7B39_004370"
     CDS             2444..2644
                     /locus_tag="N7B39_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424226.1"
                     /translation="MKIYHYTDLNGLKGIIESGSLWATHFSFLNDSNELTHGMNCLEN
                     ALQYLRDDFNPKTLKFIEQAPL"
     gene            3052..4029
                     /locus_tag="N7B39_004371"
     CDS             3052..4029
                     /locus_tag="N7B39_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HGU2424227.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTLISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PVSLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKLFCIHYRRPR
                     LKAPNDESKENPLPPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            complement(4314..5054)
                     /locus_tag="N7B39_004372"
     CDS             complement(4314..5054)
                     /locus_tag="N7B39_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU2424228.1"
                     /translation="MNNVIPLQNSPERVFLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLDGARPFVRILSEK
                     VRARRGRPPKDEVRLVPLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAV
                     RRAEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALAGHRDPRSMEVYTRVFAL
                     DMAATLAVPFTGDGRDAAEILRTLPPLR"
     gene            complement(5175..5405)
                     /locus_tag="N7B39_004373"
     CDS             complement(5175..5405)
                     /locus_tag="N7B39_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HGU2424229.1"
                     /translation="MWILNVFQVHKSVRIYIAHFSVFNCHLHPSIDRSLASWHYPNSP
                     KNGKPALSPRHDWLNTGSLLQNVPLRIWFYPQ"
BASE COUNT         1355 a         1409 c         1449 g         1230 t
ORIGIN      
        1 gtatcgtggg gtggcgagtc tcatcgtcta tggaaacgac attcgtgctg gatgcactgg
       61 agcaggcgtt gtgggcccgt cggccgtccg gcacagtcca tcacagtgat aaaggttctc
      121 agtatgtatc actggcctat acggagcgac taaaagaagc aaaactgctg gcatcaacag
      181 ggagtacagg tgactcgtat gacaacgcga tggcggagag catcaatggt ctttacaaag
      241 cggaggtaat acaccgtaag agctggaaaa accgtgcaga agtggaactg gccacactca
      301 cgtgggtgga ctggtataac aatcgacgat tgctgggaag gctgggtcat atccctccgg
      361 cagaagcaga aaaagcttat tatgcttcca tcagaaacga tgatctggca gcctgagttc
      421 acagataaaa cactctccag gaaaaccggg gcggttcata aactgcattt caccagtccc
      481 tgttctcgtc agcaaaagag ccgttcattt caataaaccg ggcgacctca gccatccctt
      541 cctgattttc cgctttccag cgttcggcac gcagacgacg ggcttcattc tgcatggttg
      601 tgcttaccag accggagata ttgacatcat atgccttgag caactgatag ctgtcgctgt
      661 caactgtcac tgtaatacgc tgcttcatag cacacctctt tttgacatac ttcgggtata
      721 catatcagta tatattctta tgccgcaaaa atcagcgcgc aaatacgcat actattatct
      781 ggcttttagt aagccttatg tattttacct ttcgttatgt taaccaataa aaattaaaat
      841 ctgccttata aaaacaaagc gtaattaccg cattcccgtt tcgtatggat ccgactttat
      901 ttgaataaac gactttccgg aagtgtacca cctgagaaaa tcatcatatc gctgttttcc
      961 gcccctgcgc ggcgcatcac agtccagaga tacaacacaa catatagtgc catgggcaac
     1021 tgaatgaggc actgcatgct gtgctgacgg gatgtaacgc tgtgcggagg gagaggaaga
     1081 tccgtaacca ccagaaaccg gatctgacca ggaaaagaga aagagtgtat gtaagttctg
     1141 tactgtatgt atgaattgta ttgtatgtat tgtgtatttt tcataccggg catacgatgg
     1201 cgccattccc tgagaaaccg gagccagacc atgacatccc tctccccgga caccgtccgc
     1261 cgcattgaag acgccgccgc cgccctgatc gccgccggca ccccgaaccc gaccaacgag
     1321 caggtccgtc agcacctcgg cggcggctcc ctgtcccaca tctccccggt gatgcgcgcg
     1381 ttccgcgccc gccagcgcga gcaggccacg cccctgccgc cggagctggc gcagctgctg
     1441 accggccagc tggggctgct gtggcaggcg gccgtgaaac aggctgaagc cggggccctg
     1501 gcggcgcgcg agcaggccga cgacgacatt gcccgggccg acaaggagcg ggatgaggcg
     1561 ctggcgaacg tggccgcgct ggaaagcgag ctggcggtgc tgcgcgaggt ggtggccgaa
     1621 cgtgaccgcc tgctgcagga ggtacgcgag ctacgcgcgg aggcgctgcc gctgcgcgag
     1681 caggtggccc gcctgaccgc caccggcgaa cacctggcgg cgcagctgca ggacacgaag
     1741 gcggagctga aggaagcccg ggaggacggc cgccagcttc agaccgaact gctggctctg
     1801 gcccgccagg acgggaaggt gaagaaatga gacaggaaag agtattacgt ataaaggcat
     1861 ttcgacgggc actggagctt ggtggcggtg aacgggctga cgggtgtcgt cattttcccc
     1921 gctggaaaac ggaactgagg aatttcccgc acagcagctg cgatttagcc agcaatactc
     1981 tggcgtacta cctcaaagaa cgagagagat gccatccgtg catcattttt atggaaggga
     2041 acgctggctt tcatgaagcg gaaaactcaa cagttcacgg gcatgtcatc gtattgctgg
     2101 acggagagta catcgatttg acgctggatc aattcgacga atatccggac tacattcctt
     2161 acgaacctgt cgaatctgac gggcagattg gtaagctgtt gcgtaatatc atgaagcatg
     2221 aggacccggt aaaaacccgc cagattgatc tcgatggtgg cgaagagctg tatgcctggt
     2281 tacgcgatac cgccgacgaa gtgctggcag cagatccgga atggcaggcc cgggaacggt
     2341 cgatagaaga agcaagggaa gccgctatta cggtgttccc gtttctgtct gacgtgcaaa
     2401 aaactgagtg cgaacagggc ccggacagtc aggaggctgc cagatgaaga tttatcatta
     2461 cactgacctg aacggcctaa agggcatcat cgaatcaggc tcactgtggg cgacgcactt
     2521 ttcctttctc aatgacagta atgagctgac tcacggcatg aactgccttg agaatgccct
     2581 gcagtacctg agggacgact ttaacccgaa aacccttaag ttcatcgaac aggcaccctt
     2641 ataagcggtt gtgtatgctg tttccctgga atgatttatc cacatatccg gaggctcgat
     2701 ccagtaaaca gatccatgaa tgatcaacaa aggatccatt aaagatcccc ataccgctgc
     2761 aaaccttgtc actcatgggc cgggaccacg atcacataag cagtggcatg ttactgataa
     2821 actgtaacat gctaatgata agctgtattc agtaatccat atactgaagt aagttaatga
     2881 cataaactat ggtcagtacg ccagactcag ctgttaaata caggctgcag gtttttcttc
     2941 agtcagttag cggggctctg acacacgatt tgctgtttat tcttttactg tccacaggca
     3001 ggaggctttc tggaaaacga aaattcagac atcaaaaaac tgttcggcga ggtggataag
     3061 tcgtccggtg agctggtgac actgacacca aacaataaca acaccgtaca acctgtggcg
     3121 ctgatgcgtc tgggcgtttt tgtaccgacc cttaaatcac tgaagaacag taaaaaaaat
     3181 acactgtcac gtactgatgc cacggaagag ctgacacgtc tttccctggc ccgtgctgag
     3241 ggattcgata aggttgagat caccggcccc cgcctggata tggataacga tttcaagacc
     3301 tgggtgggga tcattcattc ctttgcccgc cataacgtaa ttggtgacaa agttgaactg
     3361 ccttttgtcg agtttgcaaa actgtgtggt ataccttcaa gccagtcatc acgcaggctg
     3421 cgtgagcgca tcagcccttc cctgaaacgc attgccggta ccctgatctc cttttcccgc
     3481 accgatgaga agcacacccg ggaatacatc acccatctgg tacagtcagc ctactacgat
     3541 actgaacggg atattgttca gttacaggct gatccccgcc tttttgaact gtaccagttt
     3601 gacagaaagg tccttctcca gcttaaggcg attaatgccc tgaagcgacg ggagtccgcc
     3661 caggcactct acacctttat agagagcctg ccccgggatc cggcaccggt atcgctggcg
     3721 cggctgcgtg cacgcctcaa tctgaagtct cctgtatttt cccagaacca gacggtcaga
     3781 cgggcaatgg agcagctgcg cgagattgga tatcttgatt acacggagat ccagcggggg
     3841 cggacaaaac tcttctgcat tcactaccgg cgtccccggt taaaagcacc gaatgatgag
     3901 agtaaggaaa atccgttgcc accttcacct gcggaaaaag tcagtccgga gatggcggag
     3961 aagcttgccc tgcttgagaa actgggcatc acgctggatg acctggaaaa actcttcaaa
     4021 tcccgctgaa cataaactgt agtcagtgaa gagtgttcct ttactgacta cagcttatat
     4081 tatcaggtgc agtgagtggt ctgctcactg cagtttatat tcagtttcct gcagtgctgc
     4141 ctgtagctga gctgtcatct gccggtccct tacgtgagtc accccgtaac ctgatgctga
     4201 ggcattgctc ccttcataaa acatgactta ctcactacag cttatataca tgctccagct
     4261 tatgttatgt ctgttctgct gaccacagct tatataagga agcgcgtatg ccgtcacctc
     4321 aggggaggca gtgtacgcag gatctccgca gcatcccgcc cgtcacctgt gaaaggcact
     4381 gccagcgtgg cagccatatc cagcgcaaac actctggtat aaacctccat cgaacgtgga
     4441 tccctgtgac ctgccagggc ctggatgact ttccggggct ggcggtgata gagcatatgc
     4501 atgatatagc tgtgccggaa ggtgtgtggt gtgaccggaa tcgaaaagtg tactccgtca
     4561 gcttcggccc gtctgacagc ctgcttcagc cagttgcgca tggtttcgtc ggtcacggcc
     4621 cataatggtt cacgacgacg gggccgggtg gtgatcatcc agctttccat ctgcctgaca
     4681 tagcttatat ccgtcagcgg aaccaggcgg acttcatctt ttggcgggcg tccgcgtcgc
     4741 gcacgcactt tttcggacag gatccgcaca aacggccttg ctccatccag gtcaaacgat
     4801 tccggcgtca gcatccgggc ttcgccaata cgcattccgg tattccagag ggtggcgaac
     4861 agcatatggt gacgctgatc cggcatatag aaaagaaggg cactcacttc cggggccagc
     4921 aggtaggccg gtgtggcccc tgtggaagtg gccattcttc tcagggagag cgctgttgca
     4981 aaatccaccc ccggcgcaat gggtaacagg aagacgcgtt ctggtgaatt ctgcagggga
     5041 atgacattgt tcatgagtta tttttcacgt agcacacgca ctgtttatgg atacaggtat
     5101 tctgacctga acgcccggat ccttccacga caataaatcg cagcgtgcgg atccttttgt
     5161 ttgcttatca cgcattattg cgggtaaaac caaatacgta aaggtacgtt ttgcagcagg
     5221 gatcccgtat taagccagtc gtggcgcggg ctgagggctg gtttgccgtt tttgggagaa
     5281 ttggggtaat gccaggacgc taatgatcta tcaatggatg gatgtaagtg acaattgaaa
     5341 actgaaaaat gcgctatgta gattctgaca gatttatgca cctgaaatac gttcaaaatc
     5401 cacatagcgt aactctgatt ttccttacca gaaaaaagag ggg
//
LOCUS       DBFIFI010000252         5430 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.252, whole genome shotgun sequence.
ACCESSION   DBFIFI010000252 DBFIFI010000000
VERSION     DBFIFI010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.252"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..384
                     /locus_tag="N7B39_004374"
     CDS             <1..384
                     /locus_tag="N7B39_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HGU2424230.1"
                     /translation="LAEFVALISESGANPFGLTVDAVMEEYRRWRNESWRYDGSDKYP
                     WPQPVLYHICLEMRSKGIERQMTEGELKRLVERQLTKWAKHVGNGLSVPPVRRQLAAP
                     KRPPGPTPIELLKQEYERRKAAGFV"
     gene            419..1117
                     /locus_tag="N7B39_004375"
     CDS             419..1117
                     /locus_tag="N7B39_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HGU2424231.1"
                     /translation="METVFDALKALKKASSHEIAARLEISRDDAVTELWKLKRRGEAD
                     NKGSMWWLTSEATEAAPKTTAEMLINAIEQHGPQSADELALMFGITSRRANSSLAMAV
                     SKGRLIRVNQDGKYRYCIPGDNLPAEPKAASVTETDGKAFPQPAGVALPVREAETQEE
                     IKTESVAVTVQSQSSFIRKHPDGLILPSLHVANRELRRAKGQVQKWERLCAALREINK
                     HRDVIQKITTGDNG"
     gene            1139..1363
                     /locus_tag="N7B39_004376"
     CDS             1139..1363
                     /locus_tag="N7B39_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424232.1"
                     /translation="MKIRYHDFGPVSHMLISSTVLETRKHNHILDMLRLADPYLVINT
                     SGIFFLRSTVSGKTSHVLRAYKTAVREEGE"
     gene            1360..1716
                     /locus_tag="N7B39_004377"
     CDS             1360..1716
                     /locus_tag="N7B39_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="eae-like protein"
                     /protein_id="HGU2424233.1"
                     /translation="MSEINYQELRVELGAAKKRIEELEANRVVLEVENERLKHAMAVA
                     LEHISVTDAGQAGVAAMIIYDAMYHSEKTDPYAFLAELRAQGVEMVREHPAIKLCSLT
                     HICDELVAELRKGGNK"
     gene            1749..1901
                     /locus_tag="N7B39_004378"
     CDS             1749..1901
                     /locus_tag="N7B39_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424234.1"
                     /translation="MAKKHHYFAEGEITSICGGWMYFGNEREPDTFESPDDCKKCRRK
                     LNKGGK"
     gene            1898..2209
                     /locus_tag="N7B39_004379"
     CDS             1898..2209
                     /locus_tag="N7B39_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424235.1"
                     /translation="MSVIKEMPVERNEYGCWTHPEYEKFCDGREYISTEEFNAWMEKN
                     NLQWAIRSMDEDDFNLDADGPDISAWEPERPEGEGWFIGSIHDTEDGPVCVWLRNKAE
                     A"
     gene            2336..2899
                     /locus_tag="N7B39_004380"
     CDS             2336..2899
                     /locus_tag="N7B39_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424236.1"
                     /translation="MTELTKEWLQNTITGIESSRDEIPFGLDEDQNNMLTALKIALAS
                     LASVSDERAAYELFMEKRFGESVDRRRAKNGDREYMAWDMALGWIIWCHRAAMLQAGN
                     FRENKGSSTNNFRIISETSTNSPAIPDEVLSAILKVARLRADFDASEVDRRGIGSCLD
                     EAEQELIVTINEYASQIAVEATQGENQ"
     gene            3009..3113
                     /locus_tag="N7B39_004381"
     CDS             3009..3113
                     /locus_tag="N7B39_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424237.1"
                     /translation="MSDDISLVMEGALAVIAVVGVYCLVVFLMERLGN"
     gene            3357..3512
                     /gene="hokD"
                     /locus_tag="N7B39_004382"
     CDS             3357..3512
                     /gene="hokD"
                     /locus_tag="N7B39_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HGU2424238.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            3680..3958
                     /locus_tag="N7B39_004383"
     CDS             3680..3958
                     /locus_tag="N7B39_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424239.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDWYQHDPCTEEQAEW
                     LIQCYRRRGYEVKKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            3960..5009
                     /locus_tag="N7B39_004384"
     CDS             3960..5009
                     /locus_tag="N7B39_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGU2424240.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIRHAMNGTQERE
                     LSLAELSWWAACNQVVDALPEAVARRSLGLPAEKIRSVYRESDIVPGEQTAISILKQR
                     TKNIALPLHVHQQQNPPQKKTVVSIAVDPESPESFMRRPKRCRWVNEKYTRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGTKAHDIFTLPLCREHHNELYADPLEFEKKYGSQIEL
                     IFRFLDHAFATGVLG"
     gene            5022..5381
                     /locus_tag="N7B39_004385"
     CDS             5022..5381
                     /locus_tag="N7B39_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HGU2424241.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVRGQ
                     LVPGERLGIKITELECA"
BASE COUNT         1444 a         1224 c         1521 g         1241 t
ORIGIN      
        1 ctggctgagt ttgtggcgtt gatttcggag agcggggcaa atccatttgg cctgacggtg
       61 gatgccgtga tggaggagta ccgtcgctgg aggaatgaat cctggcggta cgacgggagt
      121 gataaatacc cgtggccaca gccggtgctg taccacattt gcctcgagat gcgttcaaag
      181 gggattgagc gccagatgac cgaaggggag ttaaaacggc ttgtggaaag gcagctgacg
      241 aaatgggcaa agcatgttgg taatggcctc agtgttccgc cagtccggcg acaactggca
      301 gcacccaaac gcccgccagg gccaacgcca attgaattgc tgaaacagga atatgagcgc
      361 cggaaggcgg ccggttttgt ctgagttgag aagtaatttt taccgggagg aaaatttaat
      421 ggaaaccgtt tttgacgcac tgaaagcact gaaaaaagcc tcgtcgcacg agattgcagc
      481 ccgtcttgaa atcagccgtg acgatgctgt taccgaactc tggaagctga agcgtcgtgg
      541 cgaagctgat aacaaggggt cgatgtggtg gctgacgagt gaggcaactg aagcggcccc
      601 aaaaaccact gcagagatgc tgattaacgc gattgaacag catggtcctc agtcggctga
      661 cgaactggcg ttaatgttcg ggattacctc ccgccgggcg aattcatcac tggcgatggc
      721 tgtcagcaaa ggtcgtctga ttcgcgtaaa tcaggacggt aaatatcgtt actgcatacc
      781 gggcgataat ttaccggcag agccgaaagc tgcatcggta acggaaaccg atggtaaagc
      841 ctttcctcag ccggcaggtg ttgcgttacc agtccgggaa gcggaaacac aggaagaaat
      901 taaaactgaa agtgtggcgg tcacagtgca gtcacagtcg tcgttcatca gaaagcatcc
      961 ggatggtctg attttaccat cgctgcatgt ggctaaccgc gagctgcgcc gggcaaaagg
     1021 tcaggttcag aagtgggagc gcttatgcgc cgcgctgcgg gaaattaata agcatcgtga
     1081 tgtcatacag aaaataacga caggagataa cgggtaattt ttacagcagg gtgatgatat
     1141 gaaaatcaga tatcatgatt tcggccccgt gtcacatatg cttatttcaa gtacggtgct
     1201 ggaaacaaga aaacataatc acatactgga tatgctgcgt cttgctgacc cgtatctggt
     1261 aataaacacc agtggaattt tctttctgag aagcactgtg tcaggaaaaa catcgcatgt
     1321 gcttcgggca tataaaacag cagtgcggga ggaaggagaa tgagcgagat taactatcag
     1381 gagctgcgtg ttgagctggg agcagcaaaa aaacgcatag aagagctgga ggctaatcgt
     1441 gtggtgcttg aggtggaaaa tgagaggttg aaacacgcaa tggccgtagc ccttgagcat
     1501 atatccgtca cagatgcagg acaggcaggt gtcgctgcaa tgattatcta tgatgccatg
     1561 taccacagtg aaaaaactga cccctacgct tttctggcag aattgcgggc gcagggtgtg
     1621 gagatggtgc gcgaacatcc agcaatcaaa ctttgctctt tgacgcacat atgtgacgag
     1681 ttagtcgcag agcttcgcaa aggaggcaac aagtgagtaa aacgaatccg ggctgggcaa
     1741 ggtcacttat ggcgaaaaaa catcattatt ttgctgaagg tgaaataaca agtatttgcg
     1801 gtgggtggat gtattttggc aatgagcgtg agccggatac attcgaaagt ccagatgatt
     1861 gtaaaaagtg tcgtagaaaa ttaaataagg ggggtaaatg agcgtaatta aagaaatgcc
     1921 agtggaacgt aatgaatatg gctgctggac gcatcctgaa tatgaaaaat tctgtgatgg
     1981 tcgggaatat atttcaacgg aagagtttaa cgcctggatg gagaaaaata atcttcagtg
     2041 ggctatccgt agtatggatg aagatgattt taatctggac gcagatggcc ctgatatttc
     2101 agcatgggaa cctgagcgac cagaaggtga gggatggttt atcggttcga tacatgacac
     2161 cgaagatggt cctgtttgtg tatggctgag aaataaggcc gaagcataaa ggctataaac
     2221 caacaactaa acactgaaaa tttaaatcag aaatgatttt tattaaatcc ttaaccggag
     2281 ggattcctgc accctcagaa catcaggaga ccgcccgaaa gggcggtagt gaaaaatgac
     2341 tgaattaacc aaagaatggc tacagaacac gattaccgga attgagtcat cacgggatga
     2401 aataccgttc ggactcgatg aagatcaaaa caacatgctt accgcattaa aaattgcact
     2461 ggcatcactg gcatcagtat cggatgaacg ggcagcctat gaattattta tggagaagcg
     2521 tttcggggaa tctgtagatc gccgcagagc aaaaaatggc gatagagaat acatggcatg
     2581 ggatatggcg cttggctgga ttatctggtg tcaccgcgcc gccatgcttc aggctggaaa
     2641 ctttcgggaa aataagggtt cgtcaaccaa taattttcgg ataatctcgg aaacgtcaac
     2701 caactctccg gcaatccctg atgaggtgtt gtccgcaatc ctgaaggtcg ccaggcttcg
     2761 tgcagatttc gatgcttctg aagtggacag gcgaggtatc ggtagttgtc tggatgaggc
     2821 cgagcaagaa cttatcgtta ccattaacga atacgccagt cagatcgcag tagaagcgac
     2881 acagggggag aaccaatgag ccggccagat gcatttgcag gcgttgctat agctattgct
     2941 tttctagtat atgttatttg tcggtggggg taaaaacgtt cgccgggatt cacaccaagg
     3001 gagggaatat gtcggatgat atttcactgg taatggaagg cgctctggct gttattgctg
     3061 ttgtgggtgt ttactgcctg gttgtgtttt tgatggagcg cctggggaac tgaattcatt
     3121 ccgtatggga attcccatat cgggcaaaac ggtttgctgt aaagcgagag ttaagtagaa
     3181 ttgctgcggg tgcttgaggc tgtctgcctc gggcatgcca ccgtaaggca gacagagaaa
     3241 agccccagtt aacattacgc gtcctgcaag acgcttaaca ttaatctgag gcccaatcta
     3301 tgcttcacaa acgtaggtta gcctcttacg cgccgaaagg caaggagaag caggttatga
     3361 agcagcaaaa ggcgatgtta atcgccctga tcgtcatctg tttaaccgtc atagtgacgg
     3421 cactggtaac gaggaaagac ctctgcgagg tacgaatccg aaccggccag acggaggtcg
     3481 ctgtcttcac agcttacgaa cctgaggagt aagagaccag gcgggggaga aatccctcgc
     3541 caccgctgat gtgtcaggca tcctcaacgc acccgcactt aacccgcttc ggcgggtttt
     3601 gttttttctg gtcgttctgg tttacaatcc atccgtcagc ctgaacaact ggcacctgct
     3661 gcgccagcag agaaaacaga tggcgcacga taccaaattt tacaattcgg ataactctgc
     3721 cgcccctgcc agcaggcacg ggcggcgttc tcatgcattc aaatctgact ggtatcagca
     3781 cgacccctgc accgaagaac aggctgaatg gctgattcag tgttaccgca ggcgcggata
     3841 cgaggttaag aaagccctca gtctcgatta tcgtcactgg ataatctccg tcaggcttcc
     3901 ttactccgaa cgcccaccgc gtccgtcccg cacattccag caacgcatct ggaggtaacg
     3961 tgcgggtatt acttcgacct gttctggtac cggaactcgg gctggtgatc gttaagccgg
     4021 gccgtgaatc catgccggta ttccacaata cccgggtact ggtggagccg gaaccgaaaa
     4081 gcatgcgtaa tctgccgtcc ggggtcgttc ctgccgttcg ccagccgctg gcggaggata
     4141 aatcattact gccatttttc agcgacgaac gagtgattcg tgctgctggt ggcgctggcg
     4201 cactgtctga ctggttactg cgccatgtta aatcctgcca gtggccacac ggcgattatc
     4261 accacagtga aaccgtcatt caccgttatg gtaccggcgc aatggtgttg tgctggcact
     4321 gcgacaacca gttgcgtgac cagacctccg aatcacttga gcaacttgct caccaaaacc
     4381 tgtcagcatg gatgattgac gtcattcgtc acgcaatgaa tggcacacag gagcgtgaat
     4441 tatcgctggc tgaattatcc tggtgggcgg cctgcaatca ggtggtggat gcactacctg
     4501 aggcagtagc gcgtcgttcg ctgggattac cagcggaaaa aatccgctcc gtataccgtg
     4561 agagtgacat cgtaccggga gaacagacag ccatcagcat actgaagcag cgcacaaaaa
     4621 atattgcgct gccacttcac gtccaccagc aacaaaatcc accacagaaa aaaacggttg
     4681 tcagtatcgc cgttgatccg gagtctcctg aatcgttcat gaggcggcct aaacgttgcc
     4741 gctgggttaa tgagaaatac acgcgctggg taaagacaca gccgtgtgcg tgttgtggta
     4801 agcctgctga cgatccgcat cacctgattg gtcatggtca ggggggaatg gggacaaagg
     4861 cccacgatat tttcacgcta ccgttgtgcc gggagcacca caacgaactt tatgcagacc
     4921 cgctggagtt tgagaaaaag tacggctctc agattgagtt aatttttcgt tttcttgatc
     4981 acgcctttgc gactggcgtg ctcgggtaaa agaggtgact gatgctcata gatttggttt
     5041 taccttaccc gccgacggtg aacacctact ggcgacgtcg tggcagcaca tattttgtat
     5101 caaaagccgg tgagcgttat cgccgggctg tggcgcttat tgttcgccag cagcggctga
     5161 aattaagcct gtccggaagg ctggcgataa agattattgc cgagccaccg gataagcgcc
     5221 gccgtgacct ggacaatatt ctgaaagcgc cgctggatgc gctgacgcat gcggggttgc
     5281 taatggacga tgagcagttt gatgaaatca atattgtgcg cggtcagctc gttcctggtg
     5341 agcggctggg gataaaaatc acagaactgg agtgcgcatg aataaccact atttacagtt
     5401 tgtgcgtgag ctgctcatta tcgctaccgc
//
LOCUS       DBFIFI010000253         5349 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.253, whole genome shotgun sequence.
ACCESSION   DBFIFI010000253 DBFIFI010000000
VERSION     DBFIFI010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5349
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.253"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            265..1086
                     /locus_tag="N7B39_004386"
     CDS             265..1086
                     /locus_tag="N7B39_004386"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HGU2424242.1"
                     /translation="MLNARHLPVLMYHHVSRCPGLVTLSPETFCEQMKWLADNNWRTV
                     TSAELEYFYQGGTLPRKSVMLTFDDGYLDNWLRAWPVLKEYNLHAHIFLITGLIGKGN
                     VRSRQEQEYSHRDCERLIADNRSDEVMLRWSEVREMRDSGLVEFHVHTHSHKRWDRLS
                     VSRAEQCRLMKEDILVGKQCLTEKLGFCSSHLCWPEGYYNRDYINLAGKLGFSYLYTT
                     ERRMNCPENGSLRIGRISTKEREHSGWLKRRLFYYTTPLFSSVLALHKGPRLPDN"
     gene            1086..2192
                     /locus_tag="N7B39_004387"
     CDS             1086..2192
                     /locus_tag="N7B39_004387"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HGU2424243.1"
                     /translation="MMKILFTESSSDIGGQELQALAQMTALQKQGHSVLLACREKSKI
                     APEARKRGHDVTFIPFRNSLHLPSILRLRRIIGEFKPDLVICHSGHDSNIAGLSRLIC
                     CHRFSIVRQKTYITRKTRTFSLNYLCDFIVVPSSAMMAHLMAEGVRTPVTVIPPGFDW
                     PALHNEAMRPLPLHIHAWAASADNVPLIVQVGMLRPEKGHEFMLRVLYQLKMEGKSFR
                     WLVVGAGREEYEARLRQQTEHLGMSGDVLMAGALFPALPVYRIASVVVMPSENEAFGM
                     VLAEASVSGVPVIASETGGIPDVIQKNVTGTLLPVGDVSAWTGALRDFLSRPERFRMM
                     AASAREDIEYRFDINRTAQIIVSLASQAKGKCNR"
     gene            2282..4003
                     /gene="cptA"
                     /locus_tag="N7B39_004388"
     CDS             2282..4003
                     /gene="cptA"
                     /locus_tag="N7B39_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HGU2424244.1"
                     /translation="MHLNTGQNRPTFSWSALGWAIFYFGFFSTLLQVIIFSSGYSGTN
                     GIRDSLLFSCLWLIPVFLYPDRIKIIAAVVGFILWGTSLAALCYYFLYGHEFSQSVLF
                     VMFETNAREAGEYFSQYFSLKLLLISLVYTAVSVFLWTRLRPVYIPLPWRRIVSFLLL
                     YALLLHPVVLKSLIRQEPLNDTLGKLASRMEPAAPWQFVSSYYQYHQQLNALTTFLNE
                     NSALPPLGNLRDESGERPRTLVLVIGESTQRERMSLYGYLRETTPELDALRKTDPGLT
                     VFNNVVASRPYTIEALQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTIT
                     ARNTMLTVFSRQTDRQYYMNQQRTQSAREYDTNVLKPFREVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPEGQGRFDGITGHIPTGLNAKELEVYNDYDNANLFNDHVVASLIKDFRAT
                     APDGFLLYFSDHGEEVYDTPPYKTQGRNEDNPTRPMYTVPFLLWTSEKWHAAHPRDFS
                     QYVDRKYSLAELIHTWSDLAGLTYDGYDPTRSLVNPQFRETTRWIGNPYKKNGLTDFD
                     TLPYGEP"
     gene            4077..5075
                     /gene="lpxM"
                     /locus_tag="N7B39_004389"
                     /gene_synonym="msbB"
     CDS             4077..5075
                     /gene="lpxM"
                     /locus_tag="N7B39_004389"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052689.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HGU2424245.1"
                     /translation="MVPPSAVLCYHNEISRQIPVNMKNIRTEFIPRFNLTLCFPRYWM
                     TWTGIGIICVFAMVPPALRDPLLGKLGMLVGRLGKSARQRALINLSLCFPEYSDKEKE
                     NIVDAMFATASMAVVLMAELALSGPDKISHRIRWNGLEIVEKMAQNNEKVIFLVPHAW
                     GVDIPAMLMAASGRKMAAMFHNQRNPVVDYVWNSVRRRFGGKLHARNDGIASFVRSVR
                     QGYWGYYLPDQDHGPEFSEFADFFATYKATLPVIGRLSRISGARIIPLFPVYDGKTHH
                     LTIHVSPPLAIRQKSDAHIARQINEVVENFVRPHPEQYTWILKLLKTRKEGEEDPY"
     gene            5128..5316
                     /locus_tag="N7B39_004390"
                     /pseudo
     CDS             5128..5316
                     /locus_tag="N7B39_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767718.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1364 a         1184 c         1344 g         1457 t
ORIGIN      
        1 ggatatctct cgctgtgttt actgaaattg cggtattctg ttttttctga aaaacagaaa
       61 atctgattaa aaagagcata tgcgttaagg gaaatttaag ttctggttga ttaactgata
      121 aaaatattaa aaaatccctg ttgtcggtaa tctccggacc attccgggat tacacaataa
      181 tatcaccatg tgtgatatct atggatcctc actgttgttt acgcgaggat attttcagaa
      241 tgcttaacag acgattattt ttttatgttg aatgcaaggc atcttcccgt actgatgtat
      301 catcatgtca gtcgttgtcc ggggcttgtg acactctcac ctgaaacatt ttgtgaacag
      361 atgaagtggc tcgcagataa taactggcgg acggtaacat ctgctgagct tgaatatttt
      421 tatcaggggg gaacgcttcc acgaaagagt gtcatgctca cattcgatga tggttatctt
      481 gataactggc tcagagcatg gccggtcctg aaggaatata accttcatgc acatattttt
      541 ctcattacgg ggctgattgg taagggaaac gtacgtagca ggcaggagca ggaatattct
      601 caccgcgact gtgaacggtt gattgccgat aaccgttcag atgaggtcat gcttcgctgg
      661 tctgaagtcc gggaaatgcg tgattccgga ctggttgaat ttcatgtgca cacccattca
      721 cataaacgct gggacaggtt gtctgtatca cgtgctgaac agtgccggct catgaaagaa
      781 gatattctgg tgggaaaaca atgtctgacg gaaaagctgg ggttctgtag ttcccatctg
      841 tgctggcctg aaggatatta taatcgggat tatattaatc tcgccggtaa gcttggtttc
      901 tcttatttat atacaaccga aagaagaatg aactgtcctg aaaatggttc attacgcata
      961 gggcgtatca gcaccaaaga gcgggaacat tccggctggc tgaaacgacg ccttttttat
     1021 tatacaacgc cacttttttc ttcagtactg gctcttcata aggggccaag attaccagat
     1081 aattaatgat gaaaatttta ttcactgaat cttcgtcaga tattgggggc caggagctgc
     1141 aggcactggc ccagatgact gctcttcaaa agcagggaca ttccgtttta cttgcctgca
     1201 gagaaaaaag taaaattgct ccggaagcca gaaaaagagg gcatgatgtc acttttattc
     1261 ctttcaggaa cagccttcac cttccttcca tcctcagact tcgacggatt atcggggaat
     1321 ttaaacctga tctggtcatc tgccatagtg gccatgacag taatatagca ggactgagcc
     1381 gtctgatatg ctgtcaccgc ttcagtattg tcagacaaaa aacctacatc acccgtaaga
     1441 ccagaacgtt ttccctgaat tatctgtgtg atttcattgt tgtgcctagc tcagccatga
     1501 tggcgcatct gatggcggaa ggtgtcagga caccggtaac ggttattcca ccgggtttcg
     1561 actggcccgc attgcataat gaagccatgc gaccgttacc cctgcatatc cacgcctggg
     1621 ctgcatcagc agacaatgtt ccgcttatcg ttcaggtggg gatgctacgt cccgaaaaag
     1681 gacatgaatt tatgctgcgt gtattgtatc agctgaagat ggaagggaaa tcatttcgct
     1741 ggctcgtggt gggcgccggg cgtgaagaat acgaagcacg tctgcgtcag cagacagaac
     1801 acctgggaat gagcggagat gtgttaatgg caggtgccct tttcccagcg ttaccggtct
     1861 accggattgc cagtgtggtg gtcatgcctt cggaaaatga agcgtttggt atggtactgg
     1921 ctgaagcgtc agtctccggc gtgcccgtta tcgccagtga gacgggcggg atccctgatg
     1981 tcattcagaa gaatgtgaca gggacgctgt tacccgtggg ggatgtgtct gcatggaccg
     2041 gcgcactgcg cgatttttta tcccgaccgg aacgcttccg gatgatggca gccagcgcca
     2101 gagaagatat tgagtaccgg tttgatatta acagaacggc acagatcatt gtgtctctgg
     2161 ctagtcaggc taaggggaaa tgcaacagat aacggcggat cctcttttca gcccgaattt
     2221 tgttcagcgg ttttccggta acggagtaat ttatcacatt gtttaaatac ggggacggtc
     2281 aatgcattta aataccggac agaacaggcc tactttcagc tggagcgcgc tgggatgggc
     2341 aattttttat tttggctttt tttccactct cctgcaggtc atcatcttca gcagtgggta
     2401 cagcggaacg aatggaatac gggactcact gttattcagt tgtctgtggt tgatcccggt
     2461 gtttctctat cctgatcgga taaaaataat tgcagctgtt gtcggtttca ttctctgggg
     2521 cacgtcgctg gcagcacttt gttattattt tctctatggt catgaattct ctcaaagtgt
     2581 tcttttcgtt atgtttgaaa cgaatgccag agaggctggt gaatatttca gccagtattt
     2641 cagccttaaa ctgttgctta tctcgctggt atatactgcg gtgtccgttt ttctgtggac
     2701 acgtctgcgt cctgtatata ttcctttgcc atggcggaga attgtctctt tcctgctgct
     2761 ttatgctctg cttctgcatc cggttgttct gaaatcgtta atcagacagg agccgctgaa
     2821 tgatactcta ggcaaactgg catcccgaat ggagcctgct gccccctggc agtttgtatc
     2881 cagttattat cagtaccatc agcaactgaa tgcactgaca accttcctga atgaaaatag
     2941 cgcactgcca ccactgggta atctcaggga tgaatcaggg gagagaccac gcacactggt
     3001 cctggtgatt ggtgagtcga cacagcgcga acgcatgagc ttatacgggt atctacgtga
     3061 aacgacgccg gagctggatg cactgcgtaa aaccgatccg ggtcttactg tgtttaataa
     3121 tgtggtggca tcgcgtccgt acaccattga agcattgcaa caggccctta ctttcgccaa
     3181 cgaaaagaat cctgatctgt atctgacgca gccgtcgctg atgaacatga tgaagcaggc
     3241 aggctataaa acattctgga ttaccaacca gcagacaatc acagcccgta acaccatgct
     3301 cactgtattt tcgcgccaga cggacaggca gtactacatg aatcagcagc gaacacaaag
     3361 tgcgcgtgaa tatgacacta acgtgctgaa gccgttccgg gaagtgctga atgaccctgc
     3421 accaaagaaa ctgatcatcg tgcatctgct gggtacgcac attaagtata aataccgtta
     3481 tccggaaggt caggggcggt ttgatggcat tacagggcat attcccactg gattaaatgc
     3541 gaaagagctg gaagtgtata acgattacga taatgccaat ctgtttaacg atcatgtggt
     3601 ggccagtctg ataaaggact tcagagcaac tgcgccggac ggctttctgc tttatttttc
     3661 agaccatggt gaagaagtat atgatactcc gccatataaa acgcagggac ggaatgaaga
     3721 taaccccaca cgtcccatgt acactgttcc gttcctgctg tggacctcgg aaaagtggca
     3781 tgctgcacat ccgcgagatt tttcgcagta tgttgaccgc aaatacagtc tggctgaact
     3841 gatccacacc tggtcagatt tggcgggact gacatacgat ggttacgatc cgacccgttc
     3901 tctggtgaat ccgcaattca gggaaaccac ccgctggatt ggaaatccgt ataaaaagaa
     3961 tgggctcact gatttcgaca ctcttccgta tggtgagcca tagtattttc ttatcttcgg
     4021 ttccgttctg aaggcaggag gatgtgtttt cctctgctgt ccggtggcct ccagggatgg
     4081 taccaccttc tgctgtactc tgttatcata atgagataag caggcagata ccggtaaata
     4141 tgaaaaatat cagaactgag tttattcccc gctttaacct tacattatgc ttcccccgtt
     4201 actggatgac gtggacaggt atcggtatta tctgtgtgtt tgcaatggtc ccccccgcac
     4261 tgagagatcc attgttggga aaattgggca tgctggttgg gcgtctgggg aaaagcgcac
     4321 gtcaacgggc cttgattaat ttatcgcttt gttttccgga gtacagtgat aaagaaaagg
     4381 agaatatcgt tgatgccatg tttgcaacag cctccatggc cgttgtgctt atggctgaat
     4441 tagcactgtc cgggcctgat aaaatttcgc atcgtatacg ctggaatggt ctggagattg
     4501 tggagaagat ggcacagaac aatgaaaagg tcatttttct tgtgccacat gcctggggag
     4561 tggatattcc cgcaatgctg atggctgcgt cgggcagaaa aatggcagcg atgtttcata
     4621 accagagaaa tccggtggtg gattatgtct ggaacagtgt tcgtcgtcgt ttcggaggaa
     4681 aactgcacgc acgtaatgac ggtattgcct cctttgtccg ctcagtccgg cagggatact
     4741 ggggatatta tcttcctgat caggatcatg gcccggaatt cagtgagttt gcagattttt
     4801 ttgcgacgta taaagcaact ttaccggtta tcggacggct cagcaggatc agtggtgcac
     4861 ggataatacc gcttttccct gtttatgacg gaaagaccca tcatctgact attcatgtca
     4921 gcccgccact tgctatacga cagaagagtg atgctcatat tgcgcgacag ataaacgaag
     4981 tggttgaaaa tttcgttcgt ccacatcctg agcagtatac gtggatcctc aagttgctga
     5041 aaacgcgtaa agaaggggaa gaggatcctt actgaggcag ctttttcagg ataatgagct
     5101 tacccagcaa tagtggacac acgctaagtg agtaaactct cagtcagagg tggctcatat
     5161 gacaaaaaaa cagtaccaac cagtaaaaaa ccccgtaaat agcattcgcc tgaatttcgc
     5221 agtgaagccc tgaagcttgc tgaacgcatc ggtgttgctg tcgcagctcg tgtgctcagt
     5281 ctgtatgaat cacagcttgt aagcggctcg tcagaactgt attgatattt actgagcggt
     5341 cagttcaac
//
LOCUS       DBFIFI010000254         5272 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.254, whole genome shotgun sequence.
ACCESSION   DBFIFI010000254 DBFIFI010000000
VERSION     DBFIFI010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5272
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.254"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            103..1461
                     /gene="mltD"
                     /locus_tag="N7B39_004391"
     CDS             103..1461
                     /gene="mltD"
                     /locus_tag="N7B39_004391"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HGU2424246.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNSMP
                     DS"
     gene            complement(1509..2279)
                     /locus_tag="N7B39_004392"
     CDS             complement(1509..2279)
                     /locus_tag="N7B39_004392"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGU2424247.1"
                     /translation="MTTQSHHDHVEKQFSSQASEYLTSTVHASGRDLQLLAVCLADYP
                     DASVLDMGCGAGHASFVAAQNVSTVVAYDLSAQMLDVVAQAAEVRQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSPGHPVRDIWL
                     QTVEALCDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFSSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVDAHKAA"
     gene            complement(2357..3157)
                     /locus_tag="N7B39_004393"
     CDS             complement(2357..3157)
                     /locus_tag="N7B39_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HGU2424248.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            3398..4312
                     /gene="yafC"
                     /locus_tag="N7B39_004394"
     CDS             3398..4312
                     /gene="yafC"
                     /locus_tag="N7B39_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HGU2424249.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPVACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDREIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(4309..5112)
                     /gene="dkgB"
                     /locus_tag="N7B39_004395"
     CDS             complement(4309..5112)
                     /gene="dkgB"
                     /locus_tag="N7B39_004395"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HGU2424250.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGLAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKEGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRENLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT         1381 a         1267 c         1314 g         1310 t
ORIGIN      
        1 atggttaagg tcaaagaaag ataggttctg ataaaacttt cttgtcatcg gctccgttcg
       61 ccgttatgat cggtcgtctt ttaagcaact attgacgcac acatgaaggc aaaagcgata
      121 ttactcgcct ctgtcctgct cgtgggttgc cagagtaccg gcaacgttca acagcacgca
      181 cagagccttt ctgcagctgg tcaaggggaa gcagcaaagt ttacaagtca ggcacgatgg
      241 atggacgatg ggacgtctat cgcgccagat ggtgacttgt gggctttcat tggcgacgag
      301 ctaaagatgg gaattccgga aaatgaccgg attcgcgaac agaaacagaa atatttacgc
      361 aataagagct atctccacga tgtaacttta cgggcagagc cgtatatgta ctggatagcc
      421 gggcaagtta aaaaacgtaa catgcctatg gaactggtac tactacccat agtggagagc
      481 gcttttgatc ctcacgcaac gtctggcgcc aatgccgcag gcatctggca gatcattccg
      541 agcacggggc gcaattatgg tttgaaacag acccgcaatt atgacgcgcg tcgcgatgtt
      601 gttgcttcaa caactgccgc gctgaatatg atgcagcgtc tgaacaaaat gtttgatggc
      661 gactggcttc tgaccgtagc ggcttataac agcggcgaag gtcgggtcat gaaggcaatt
      721 aaaacgaaca aagcgcgtgg gaaatccacg gacttctggt cgttaccgtt gccgcaggaa
      781 acgaagcagt acgtgcctaa aatgctggca ttgagtgata ttctcaaaaa cagcaagcgt
      841 tatggcgtac gtctgccaac gaccgatgaa agccgtgctc tggcgcgtgt gcacctgagt
      901 agcccggttg aaatggcgaa ggttgcagat atggcgggga tttccgtcag caagctgaag
      961 acattcaacg ctggcgtgaa aggctccacg ctgggcgcaa gtggtccgca gtacgtgatg
     1021 gtgccaaaga agcatgcaga tcaactgcgt gaatctctgg cttcaggcga aattgctgct
     1081 gtacagtcga cgctggttgc cgacaatacg ccgcttaaca gccgtgttta caccgtacgc
     1141 tctggcgaca cgctttcaag tatcgcttca cgtctcggcg taagcaccaa agatttgcag
     1201 cagtggaaca aactgcgcgg ctctaagctg aaaccaggcc aaagtctgac gattggcgca
     1261 ggtagtagcg cacagcggtt ggcaaacaac agcgatagca ttacgtatcg tgtgcgcaaa
     1321 ggcgattcgc tttcaagcat tgctaaacgc cacggcgtga acatcaaaga tgtgatgcgc
     1381 tggaacagcg atactgcgaa tctgcaacca ggcgataagc tgacgttgtt tgtgaaaaac
     1441 aacagcatgc cagactcctg acaaaccaga taatagaaag gcaccgattc ccccggtgcc
     1501 ttttttattt atgccgcttt atgtgcatct accatgatga tatcactggt gaaagagcca
     1561 tcattctgca aggcaaaata cgttctcacc tctgtcgatg cgctctgttg gtaaatgcga
     1621 atagcgtcta ctaacgcttc tggcgtacgc attctcgcga cccatgaaga aaattccagc
     1681 ggtaacttat ctgtaattaa attatcaact atcagattgg cttcattgat taacctcaac
     1741 cactcaccgc tggcgtagtt tcgtacgtga gaggtatcgc ataatgcttc taccgtctgt
     1801 aaccagatgt cgcgcactgg gtggcccgga gacattacgt ccatcacaat cagcctaccg
     1861 ccaggtttca atatccggtt cacttcacgc agtgctgcac caacatcatg ccagtgatgg
     1921 gcagaataac ggctgataac aatatcaaat gcgttatcgg caaatggcag actttcggca
     1981 tacccctggc gggtggtgat atttttcagt tgccggactt cggcagcttg tgccacgaca
     2041 tccagcattt gggcagataa gtcatacgcc accaccgtgc tcacattttg cgcagcgaca
     2101 aagctggcat gccctgctcc gcagcccata tcaagtacac ttgcatcagg ataatcagcc
     2161 agacacaccg ccaggagctg taaatctcgt ccggatgcat gtacggtact ggtcagatat
     2221 tcgctggctt gagagctaaa ctgcttttct acgtggtcat ggtgggattg tgttgtcatt
     2281 gcactgtcct tttgttgttt gagtataaaa gggcagctcc aaacgttggt ctgccctgtg
     2341 gcagacctga cataccttat ttatcaggct tgccgggact gaattcaacg agtagcggat
     2401 tgtgatcgga cgcgcgcgta accagtacag aagcttcgct gacgttcaga ccacggtaga
     2461 aaacaaaatc gagcgggcga ccaaacgccc gacggcgctg atcatcggta aaacgcacct
     2521 gacgcagcga catttcccgc gcaaaacgat ataacgcgtt catccttcta cggctccaag
     2581 cattgaaatc tcccgccata atgaccgggc cgctgtggtg agctatctga tcaccaatag
     2641 gaagtaactg cttactataa acatccacgc ccagactgaa gttgacggcg tgtatattaa
     2701 ccaccattaa caggcgggtg tcaggtaatg gatagaccgt caccagtgcc gacttcgcca
     2761 gacgcaaaat gggttctcgt tcacgtaacg ggcagcaata cactggatgt gccgccgaaa
     2821 gggtcattac gccagaagga tgttgtggca gcacgaaagc gggtacctga tcggcggcaa
     2881 gatagttagc agtcgcaaac tgtaccaact ctggcgttgt ttgcgcttcc tgcaataaca
     2941 ccagatgtgc gtctttgccg taattcttta ataccgacaa ccattcagcg cgttgctgtt
     3001 tgtatatgtt ccacaccagg atccgaatac gctcttcggt acttaacggt tctccgggtg
     3061 gtaatgcctg gccgatgctc gcaaaagacc ccggcggtaa gatcctctcc gcaggttgtc
     3121 cggcaacata gcgcatggca taggtgtttt ttcgcacttt tgactttgaa acctctatta
     3181 cgtcaaccct tccgcactcg ggagggttct actgttatag ggattttagc tcactctttc
     3241 aacgagcaat tgtatattgt tatgtaagca agtgcttaca ccggtcagaa ttttgctcat
     3301 tattgagaac attaaatcgg atgacatatc acgcatcctg atggtttaat gacaaataaa
     3361 ttcaaaatat ctcatctatt ttgcaacagg agcaaaaatg aaagccacgt cggaagaact
     3421 cgccattttt gtttcggtcg tcgaaagcgg cagctttagc cgggcagcgg aacaattagg
     3481 gcaagcaaac tcagcggtaa gccgggcggt gaaaaagctg gagatgaaac ttggcgttag
     3541 cctgcttaat cggaccacgc ggcaacttag cctgacggaa gaaggcgagc gttattttcg
     3601 tcgcgtacag tcaattttgc aggagatggc agcggcagaa tcagaaatta tggagacgcg
     3661 taatacaccg cgtggactgt tgcgcatcga tgccgcaact ccagtggtgc tgcactttct
     3721 gatgccgttg attaagcctt ttcgtgaacg ctatccggaa gtcactttat cgctagtctc
     3781 ctccgaaacg attattaatt tgatcgaaag aaaagtggat gtcgcgatac gcgccggtac
     3841 gttaacggat tccagcttac gtgccaggcc gttatttaac agttatcgaa aaattatcgc
     3901 ctcccccgat tatatttccc gctacgggaa gccagaaacg atcgacgatt taaagcaaca
     3961 tgtttgcctg ggattcactg aacccgcttc cctcaatacc tggccggtag cctgtagcga
     4021 tggacaatta catgaggtga agtacggttt gtcatccaat agtggggaaa cactgaaaca
     4081 gctttgcctg agtggcaacg ggattgcgtg tttgtccgac tatatgatcg acagagaaat
     4141 cgctcgcgga gaattggtgg agttaatggc agataaagtg ttgccagtgg aaatgccatt
     4201 cagtgccgtc tattacagcg accgtgcggt aagtacgcgc atccgggctt ttatcgattt
     4261 ccttagcgag catgtaaaaa cagctcccgg aggagctgtc agagaggctt aatcccattc
     4321 aggagccaga ccttccgggc taaccaggcg gtcgttgcaa tccagtgcgg cgatcgcttt
     4381 tttatcttcg gcatcaagct gtaaattttg tgccttaaga ttactctcca ggttttcacg
     4441 tttagtagaa gaaggaatta ctgagtaacc ttcccccata gcccacgcca gaatcacttg
     4501 tgccggagtc gcattgtgtt tagctgcgat acgagcaata acctcatctt tcagggcctt
     4561 accatacgcc agcgtcatat aggaagtaat atggatgccg tgctgtttag cccaggcaac
     4621 cactttacgg ttttgcagat aaggagagag ttcaatctgg ttagtagcga tgttttcagc
     4681 gccaacagca gcaatcgcct tttccatcaa tgggatcgtg aagttggaaa taccgatctc
     4741 acgcgtcaac ccttcttttt tggcttccag cagcgcctgc ataaactctt caacagagac
     4801 ttcatcgttt ggtgacggcc agtggattag cgtcagatca acataatcgg tacgcaattt
     4861 ttgcaggctc tcttttagac tcgggatcaa tttgtctttg ctgagatttt caatccagat
     4921 tttagtggtg atgtagagtt catgacgtgg cacgccactt tctgcaatcg ccagacctac
     4981 tgcggcttcg ttatcataga tttgtgcagt atcaattgcg cgataaccaa gttcaagcgc
     5041 cgttttcaca gatgaaataa caacgtcgtc tttcagacgg aaagtaccta aaccaaatgc
     5101 agggatagcc ataatatgcc tcttttaatg ctattttcgt taactgaggt gattatgggc
     5161 tttagaatgt tgcggaaaaa gggcgtaaaa cgcagaggat ttttgcgatt ttagcaataa
     5221 tagaaatgta gacgaaaaaa aacctcgagc gataactcga ggcttcttaa ta
//
LOCUS       DBFIFI010000255         5263 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.255, whole genome shotgun sequence.
ACCESSION   DBFIFI010000255 DBFIFI010000000
VERSION     DBFIFI010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5263
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.255"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..546
                     /locus_tag="N7B39_004396"
     CDS             <1..546
                     /locus_tag="N7B39_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HGU2424251.1"
                     /translation="KTGAEAIRALAMQIPAFRQKLSDGWYLVRIAGRDTGENELSARL
                     NEPLANGAVIHIVPRLAGAKSGGVFQAVLGAALIAVAWWNPVGWLGAAAVSGMYAAGA
                     SMILGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMVAQGNVLPVLYGEMRVGSRV
                     VSQEISTADEGDGGQVVVIGR"
     gene            607..4020
                     /locus_tag="N7B39_004397"
     CDS             607..4020
                     /locus_tag="N7B39_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HGU2424252.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALRETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYDPEKRTYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWTDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEGIVDNGAHFDGDQSSTVNGVTPPAVQHL
                     TAEVSADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSQIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSETRIADIRQVETSARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGRASDDAEGYLDFFKGKITESHLGKELLEKVDLTEDNASRLDEFSKEWKDAN
                     DKWNAMWGVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETP
                     MFVAQGNQIFMNDVFLKRLTAPTITSGGNPPVFSLTSDGKLTAKNADISGSVNANSGT
                     LNNVTVNENCTIKGMLEATQVRGDFVKAVSKSFPKQAGTWGNTETPNGTVTVTISDDH
                     NFDRQIIIPPIIFNGIAYSDPGSGNNPGGTRYTGYGFEVRKNGVLIASRETKGAIPGS
                     YSAVIDMPSGRGSVTLEFKVFHKGNQRAGNITDCTVIVTKKAASGISIR"
     gene            4091..4690
                     /locus_tag="N7B39_004398"
     CDS             4091..4690
                     /locus_tag="N7B39_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HGU2424253.1"
                     /translation="MRKVCAAILSAAICLAVSGVPAWASEHQSTLSAGYLHASTDAPG
                     SDDLNGINVKYRYEFTDTLGLITSFSYANAEDEQKTHYSDTRWHEDYVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYFRVTDNKRKTHDVLTGSDDARYSNTSLAW
                     GAGVQFNPTESVAVDVAYEGSGSGDWRTDGFIVGVGYKF"
     gene            4750..>5263
                     /locus_tag="N7B39_004399"
     CDS             4750..>5263
                     /locus_tag="N7B39_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HGU2424254.1"
                     /translation="MAAVQISGVLKDGAGKPIQNCTIQLKARRNSTTVVVNTVASENP
                     DEAGRYSMDVEYGQYSVTLLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMSEDDVRPE
                     ALRRFELMVEEAARHAEEAKKNAGEAETSARNAGISASQAEESAANADTSAGDASESA
                     RQAAESAAAAK"
BASE COUNT         1239 a         1277 c         1673 g         1074 t
ORIGIN      
        1 aaaacggggg ccgaagccat ccgggcgctg gccatgcaga tcccggcgtt tcgtcagaag
       61 ctgagcgacg gctggtatct ggtacggatt gccgggcgtg atacaggtga aaatgaatta
      121 tctgcccgtc ttaatgagcc gctggcaaat ggtgccgtga tccacatcgt gccgcgcctg
      181 gcgggtgcta aaagtggcgg tgtgtttcag gcagtgctgg gtgcggcgct gattgcggtg
      241 gcatggtgga accctgtggg ctggctgggg gccgcggctg tatcgggtat gtatgcagca
      301 ggggccagta tgatcctggg tggtgtggcg cagatgctgg caccgaaagc cagaactccc
      361 cgtacacaga caacggataa cggcaaacaa aacacctatt tctcctcact ggataacatg
      421 gttgcccagg gcaatgttct gcccgttctg tacggtgaaa tgcgcgtggg atcacgtgtg
      481 gtttctcagg agatcagcac ggcagacgaa ggggacggtg gtcaggttgt ggtgattggt
      541 cgctgatgca aaatgtttta tgtgaaaccg cctccgggcg gttttgtcgt ttatggagca
      601 tgacgaatgg gtaaaggaag cagtaagggg cataccccgc gcgaagcgaa ggacaacctg
      661 aagtccacgc agctgctgag tgtgatcgat gccatcagcg aaggaccgat tgaaggtccg
      721 gtggatggat taaaaagcgt gctgctgaac agtacgccgg tgctggacag tgaaggtaat
      781 accaacatct ccggtgtcac ggtggtgttc cgggccggtg agcaggagca gacaccgccg
      841 gagggatttg aatcctccgg ttctgagacg gtgctgggta cggaagtgaa atacgacacg
      901 ccgatcaccc gcaccatcac gtcggcaaac atcgatcgtc tgcgctttac tttcggtgtg
      961 caggcactgc gggaaaccac ctcaaagggg gaccggaatc cgtcggaagt ccgcctgctg
     1021 gttcagatac agcgtaatgg tggctgggtg acggaaaaag acatcaccat taagggcaaa
     1081 accacgtcgc agtatctggc ctcggtggtg gtggataacc tgccgccgcg cccgtttaat
     1141 atccggatgc gcaggatgac gccggacagc accacagacc agctgcagaa caaaacgctc
     1201 tggtcgtcat acaccgaaat catcgatgtg aaacagtgct acccgaacac ggcactggtt
     1261 ggcgtgcagg tggactcgga gcagtttggc agtcagcagg tgagccgtaa ttatcatctt
     1321 cgcgggcgca ttctgcaggt gccgtcaaac tatgatccgg aaaaacgcac ttacagcggc
     1381 atctgggacg gaacgttaaa accggcatac agcaacaaca tggcctggtg tctgtgggat
     1441 atgctgaccc acccgcgcta cggcatgggg aaacgtcttg gtgcggcgga tgtggataaa
     1501 tgggcgctgt atgtcatcgg ccagtactgc gaccagtcag taccggacgg ctttggcggc
     1561 acggagccgc gcatcacctg taatgcgtac ctgaccacgc agcgcaaggc gtgggatgtg
     1621 ctcagtgatt tctgctcggc gatgcgctgt atgccggtat ggaacgggca gacgctgacg
     1681 ttcgtgcagg accggccgtc ggataaggtg tggacctata accgcagtaa tgtggtgatg
     1741 ccggatgatg gcgcgccgtt ccgctacagc tttagcgccc tgaaagaccg ccataatgcc
     1801 gttgaggtga actggactga cccggacaac ggctgggaga cggcgacaga gcttgtggag
     1861 gacacgcagg ccattgcccg ttacggtcgt aacgtcacga agatggatgc ctttggctgt
     1921 accagtcggg ggcaggcaca ccgtgccggg ctgtggctga ttaaaacgga actgctggaa
     1981 acgcagaccg tggacttcag cgtgggcgca gaagggcttc gccatgtacc gggcgatgtc
     2041 attgaaatct gtgatgatga ctatgcgggg atcagcatcg gtgggcgtgt gctggcggtg
     2101 aacagccaga cccggacgct gacgctcgac cgtgaaatca cgctgccatc ctccggcacc
     2161 acgctgataa gcctggttga cggaagtggc aatccggtca gcgtggaggt tcagtccgtc
     2221 accgacggcg tgaaggtaaa agtgagccgg gttcctgacg gtgttgctga atacagcgtg
     2281 tgggggctga agctgccgac gctgcgccag cgcctgttcc gctgcgtgag tatccgtgag
     2341 aacgacgacg gcacgtatgc catcactgcc gtgcagcatg tgccggagaa agagggcatc
     2401 gtggataacg gggcgcactt tgacggtgac cagagcagca cggtgaatgg tgtcacgccg
     2461 ccagcggtgc agcacctgac cgccgaagtc tccgcagaca gcggggaata tcaggtgctg
     2521 gcgcgatggg acacgccgaa ggtggtgaag ggtgtgagct tcctgcttcg cctgaccgtg
     2581 gcagcggatg acggcagtga gcggctggtc agcacggccc ggacgacgga aaccacatac
     2641 cgcttcacgc agctggcgct ggggaactac aggctgacag tccgggcggt aaatgcgtgg
     2701 ggacagcagg gcgatccggc gtcggtatcg ttccggattg ccgcaccggc agcgccgtca
     2761 cagattgagc tgacaccggg ctattttcag ataaccgcca cgccgcatct tgcggtttat
     2821 gatccgacgg tacagtttga gttctggttc tcggaaacgc ggattgcgga tatcaggcag
     2881 gttgaaacca gcgcgcgtta tcttggtacg gcgctgtact ggatagccgc cagtatcaat
     2941 atcaaaccgg gccatgatta ttacttttat atccgcagtg tgaacaccgt tggcaaatcg
     3001 gcattcgtgg aggccgtcgg tcgggcgagc gatgatgcgg aaggttatct ggattttttc
     3061 aaaggcaaga taaccgaatc tcatctcggc aaggagctgc tggaaaaagt cgatctgacg
     3121 gaggataacg ccagcagact ggatgagttt tcgaaagagt ggaaggacgc taacgataaa
     3181 tggaatgcca tgtggggcgt caaaattgag cagaccaaag acggcaaaca ttatgtcgcg
     3241 ggtattggcc tcagcatgga ggacacggag gaaggcaagc tgagccagtt tctggttgcc
     3301 gccaatcgta tcgcgtttat tgacccggca aacgggaatg aaacgccgat gtttgtggcg
     3361 cagggcaacc agatattcat gaacgacgtg ttcctgaagc gcctgacggc ccccaccatt
     3421 accagcggtg gaaatccacc ggtattttcc ctgacatcag acggaaagct gaccgctaaa
     3481 aatgcggata tcagtggcag tgtgaatgcg aactccggga cgctcaacaa cgtcacggta
     3541 aatgaaaact gtacgattaa gggcatgctg gaggcgactc aggtcagagg tgacttcgtt
     3601 aaagctgtat ccaaatcatt tccgaaacag gctggtacgt ggggtaacac ggaaacacca
     3661 aacgggacgg ttacagtcac catcagcgat gatcataact ttgaccgtca aatcattatt
     3721 ccgcccatta tctttaacgg aatagcgtat agcgatccgg gaagtggtaa taacccggga
     3781 ggtacaagat acacgggtta tggttttgaa gttcgcaaaa acggtgtatt aatcgcatcc
     3841 agagaaacta aaggggccat tcccggtagc tacagtgcgg ttattgatat gccgagtggc
     3901 aggggaagcg tcactctgga gtttaaggtt ttccataaag gcaatcagcg ggcaggtaat
     3961 atcaccgact gtacggtgat tgtgaccaaa aaagcggctt ccggcatcag tatccgttga
     4021 aatatttata accctaataa cgggcgccag aaatggcgcc ttttttattg cagaaaatcg
     4081 ggaggtaatt atgcgtaaag tttgtgcagc aattttgtcc gcagccattt gtctggccgt
     4141 atccggtgtg cctgcatggg cgtctgagca tcagtccacg ctgagcgcag gatatcttca
     4201 tgccagtacg gacgctcccg gcagcgatga tctgaacggg attaacgtga aataccgtta
     4261 tgagtttacg gacacactgg ggctgattac gtccttcagt tatgccaacg ccgaagatga
     4321 gcaaaaaacg cattacagcg atacccgctg gcatgaggat tacgtgcgta accgctggtt
     4381 cagcgtgatg gcagggccgt ctgtgcgtgt gaatgaatgg ttcagtgcgt atgcgatggc
     4441 gggtgtggct tacagccgtg tgtcgacttt ttccggggat tatttccgcg taactgacaa
     4501 caagaggaaa acgcacgacg tgctgaccgg aagtgatgac gctcgctaca gcaacacatc
     4561 tctggcgtgg ggggctggcg tgcagtttaa cccgaccgaa tcagtggccg ttgatgtcgc
     4621 ttatgaaggc tccggcagcg gtgactggcg cactgacggt ttcatcgtgg gtgtcggtta
     4681 taaattttga ttagtaacac agcgttatga cagcccgccg gttcaggcgg gcttttttgt
     4741 ggagtgggta tggcagcagt acaaatatca ggcgtgctga aagatggtgc gggaaaacca
     4801 atacagaact gcaccattca actgaaagcc agacgtaaca gcaccacggt ggtggtgaac
     4861 acggtggcct ctgaaaatcc ggatgaggca gggcgttaca gcatggacgt cgagtatggt
     4921 cagtacagcg tcactctgtt ggtggaggga ttcccgccat cacatgccgg gaccatcacc
     4981 gtgtatgaag attctcaacc ggggacgctg aatgattttc tcggtgccat gtcggaggat
     5041 gacgtccggc cggaggcact gcgtcgtttt gaactgatgg tggaagaagc ggcgcgtcac
     5101 gctgaggagg cgaagaagaa tgccggagag gcggagacgt ccgcgaggaa tgccggcata
     5161 tcagccagtc aggcagaaga gagcgcggca aatgctgaca cttcagcagg ggatgcatcg
     5221 gagtcagccc ggcaggcggc agaaagtgca gccgctgcaa agc
//
LOCUS       DBFIFI010000256         5235 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.256, whole genome shotgun sequence.
ACCESSION   DBFIFI010000256 DBFIFI010000000
VERSION     DBFIFI010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5235
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.256"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..388)
                     /locus_tag="N7B39_004400"
     CDS             complement(<1..388)
                     /locus_tag="N7B39_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU2424255.1"
                     /translation="MRRKREEIASPEATQRLRAIWDAKKRELKLTQELAAELMGFEAQ
                     SAVSHYLNGKAPLNTDAALKFAVLLRVKPEELRPDLADLMNYVRSSGTYDESFEGEGW
                     RMVNKQQADLLDLFDILPESEKAKLIE"
     gene            493..768
                     /locus_tag="N7B39_004401"
     CDS             493..768
                     /locus_tag="N7B39_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU2424256.1"
                     /translation="MTLDEYLKKNRVRQSCLAALAGCSQSMISLVATGRSQLSPEKVL
                     RIAEATNFEVTPHELRPDIYPNPTDGLPVGCKANTQNAQELIHENQA"
     gene            752..1174
                     /gene="ydaT"
                     /locus_tag="N7B39_004402"
     CDS             752..1174
                     /gene="ydaT"
                     /locus_tag="N7B39_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HGU2424257.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELPLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSPGDSVVVH"
     gene            1252..2040
                     /locus_tag="N7B39_004403"
     CDS             1252..2040
                     /locus_tag="N7B39_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424258.1"
                     /translation="MASNWIKLEVITPDKPEIFRLAEILNIDPDAALGKVIRFWAWAD
                     QQMIDGNADCNARGVTKSAIDRITFMAGFADALIQVGWLVENDGGLSLPNFERHNGKS
                     SKKRAVTNERVTKIRELKRKGNAGSVTQTDQKALPEEEEEEDINTDLPLNPPRQKRAS
                     KKFEPEAIELPDWLPETLWHEWVQFRQALRKPIRTEQGANGAIRELEKFRQQGFTPEQ
                     VIRHSIANEYQGLFVPKGVRPETLLRQVNTVSLPDSAIPPGFRG"
     gene            2047..2793
                     /gene="ydaV"
                     /locus_tag="N7B39_004404"
     CDS             2047..2793
                     /gene="ydaV"
                     /locus_tag="N7B39_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGU2424259.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLTEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYQVQNDGQRYALSQAKSIADELMTGCT
                     NFAFSGKPGTGKNHLASAIGNRLLKDGQTVIVVTVADVMSALHASYDDGQSGEKFLRE
                     LCEVDLLVLDEIGIQRETKNEQVVLHQIVDRRTASMRSVGMLTNLNYEAMKTLLGERI
                     MDRMTMNGGRWVTFNWDSWRPNVSNMRVVK"
     gene            2815..3585
                     /locus_tag="N7B39_004405"
     CDS             2815..3585
                     /locus_tag="N7B39_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HGU2424260.1"
                     /translation="METVSDALKALKKASSHVVAARLGISREEAVNELWELKRNGVVD
                     KTGHTWFLAGEGESRVTEERPVKSEAQDMLTGEVEQKVTADMMIEFIGQDGAKTCEEL
                     ADKFGVSIRKVASTLAVVTATGRLARVNQNGKFRYCMPGGNLPADPKAAPVTENDGKA
                     FPQPAGAALPVREAATQEEIKTETVADIVQPLPSFTETQADELIFPSLRRANLALRRA
                     KGQVQKWERVCAALRELNKHRDIVRQIVDSSGRIVSEK"
     gene            3601..3996
                     /locus_tag="N7B39_004406"
     CDS             3601..3996
                     /locus_tag="N7B39_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HGU2424261.1"
                     /translation="MAKPFTHEQREELKARIIGLVRKNERMTISQLERATGAGWHSVR
                     RCLVDVLACGDLYMPGKYGVFTSEQVYRVWRKAAEKTTDQTLIRKLPDGEIRRYDRQQ
                     NIICGECRKSEVMLRVLAFYQGNFQEAIL"
     gene            4054..4410
                     /locus_tag="N7B39_004407"
     CDS             4054..4410
                     /locus_tag="N7B39_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424262.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEASERAYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEERERRKNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            4504..4677
                     /locus_tag="N7B39_004408"
     CDS             4504..4677
                     /locus_tag="N7B39_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424263.1"
                     /translation="MRKFKIIIETGIAGGDFEDEFEVDDDATPDEIHDEAKDIFFNYC
                     NYSYHEIKDEEEE"
     gene            4679..4855
                     /locus_tag="N7B39_004409"
     CDS             4679..4855
                     /locus_tag="N7B39_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424264.1"
                     /translation="MADFGSTKYSVSFEEWHELLMDYAELRGGSAADAEAWRDDYEAG
                     KTAVEAYCDEWGDE"
     gene            4852..>5235
                     /locus_tag="N7B39_004410"
     CDS             4852..>5235
                     /locus_tag="N7B39_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HGU2424265.1"
                     /translation="MSEIDYQALREAAEKATKGCYIVGHTSGNQHGNITGVFVCQKWK
                     GEPGGVIAECHVNCLVETDAQAYANAEFIAAFNPNVALALLDERERNQQYIKRRDQEN
                     EDIALTVGKLRVELEAAKSKLNEQRE"
BASE COUNT         1487 a         1090 c         1442 g         1216 t
ORIGIN      
        1 tttcgattag ttttgctttt tctgattcgg gaaggatatc aaaaagatct agtaaatcag
       61 cttgttgctt gttaaccatc cgccaacctt cgccttcgaa actttcgtca taagttcctg
      121 aggaacggac gtaattcatc agatcagcta aatcaggcct caactcttca ggcttaactc
      181 ttagtaatac agcgaatttt aatgccgcat cagtattaag tggagccttt ccgttgagat
      241 aatggctaac cgcagattgc gcctcaaaac ccattagctc ggcagcaagc tcctgagtca
      301 gtttgagctc tctttttttt gcatcccaga ttgcgcgcag acgttgcgta gcttccggcg
      361 atgcgatttc ttcgcgtttt cttctcatac caccatctta tgaatacagt tcataatctc
      421 aaactgatat aggtattgat catttaaatt agtatggtta atatttttgt gagcattact
      481 aaggtgacca ttatgacatt agatgaatat ttgaaaaaaa atcgtgtacg acagtcttgt
      541 ttggccgcgc tggctggttg ttcgcaatca atgattagcc tcgttgctac tggacgtagt
      601 cagttaagcc ctgaaaaggt attgcgtatc gcagaggcta cgaatttcga ggttacacct
      661 catgaactcc ggcctgatat ctaccctaat ccgaccgatg gtttacctgt tggatgtaag
      721 gctaacacgc aaaatgcaca ggagttgatt catgaaaatc aggcatgagc acatcgaatc
      781 agtgttgtta gccctggcag ccgaaaaagg gcaggcgtgg gtcgctaacg caattactga
      841 agaatatctg cgccaggggg gcggcgaatt gcccctggta ccaggcaagg actggaataa
      901 tcagcagaac atctatcacc gttggttaaa aggtgaaacg aaagcgcaaa gggaaaaaat
      961 tcagaaactg atccctgcgg ttctggcaat tcttccgcgc gagctgcgtc accgactctg
     1021 catcttcgat accctggaac gccgtgcatt actggcggcg caggaagcgt tgagtacggc
     1081 aattgatgcg catgatgatg cagtccaggc cgtttaccgg aaagcacatt tcagcggtgg
     1141 tgggtcgccc ggcgattctg tcgtagtgca ttgattgaaa ttaatcgtgc cggattgttt
     1201 tgttcggtat cagttaaatg taacgctgcg agcgttacaa ggtgaaaaca aatggcttca
     1261 aactggataa agctcgaggt tattacgccg gataagccgg aaatattcag gcttgctgag
     1321 attctgaata ttgatccaga tgccgcatta gggaaggtta ttcgcttctg ggcatgggcg
     1381 gatcaacaaa tgatagacgg taatgcagat tgtaacgctc gcggcgttac aaaaagtgca
     1441 atagatcgca tcacttttat ggctggtttt gctgatgcgt taattcaggt tggatggctg
     1501 gtcgaaaatg acggtgggct ttctctacct aactttgaac gtcataacgg gaaaagctct
     1561 aaaaaacggg cggttacaaa cgagcgagtt acaaaaatac gcgaactgaa acgaaaaggt
     1621 aacgctggca gcgttacaca aacggatcaa aaagcgttac cagaggaaga ggaagaggaa
     1681 gatataaata ctgatctccc cctaaatccc cctcgccaaa aacgagcgtc taaaaaattc
     1741 gagccggagg ctatcgagct gcctgactgg ttgccggaaa cactctggca tgagtgggtt
     1801 cagttcaggc aggcattgcg aaaaccgatt cgaacggagc agggcgctaa cggggcgata
     1861 cgggaactgg aaaaattccg tcagcagggt tttacacctg agcaggtgat tcgacacagc
     1921 atcgccaatg aataccaggg cttgttcgta ccgaaaggtg ttcggcctga gacgttgctc
     1981 cgacaggtta acaccgtctc gttgccggac agtgcgatcc cgccaggctt cagggggtaa
     2041 cagaccatga aaaatattgc gacaggcggc gttctggagc gtatccgcag actgacccca
     2101 ccacatgtaa ccgccccatt cagaacggtt gcggagtggc gtgagtggca acttactgaa
     2161 ggccagaaac gttgcgagga gatcaaccgt ctgaatcgtc agttgcgggt ggaaaaaatt
     2221 ctgaatcgct caggcatcca gccgttgcac cgtaaatgct cgtttgcgaa ttaccaggtg
     2281 cagaacgacg gccagcgata cgcgttaagc caggcgaaat ccatcgccga tgaactgatg
     2341 accgggtgta caaattttgc gttcagcgga aaacctggta ccggaaagaa tcacttagcg
     2401 tcagctatcg ggaatcgcct gctgaaagac ggtcagacag tgattgtggt taccgtggct
     2461 gatgttatga gcgccctgca cgccagctat gacgacgggc agtcaggcga aaaatttttg
     2521 cgggagctgt gcgaagtgga tctgctggtt cttgatgaaa ttggcattca gcgcgagacg
     2581 aaaaacgagc aggtggtgct gcaccagatt gttgatcgcc ggacagcgtc gatgcgcagc
     2641 gtggggatgc tgacaaacct gaactatgag gccatgaaaa cattgctcgg cgagaggatt
     2701 atggatcgca tgaccatgaa cggtggtcga tgggtgacgt ttaactggga tagctggcgt
     2761 ccaaatgtca gcaatatgag ggttgtgaag taattttgtc cggaggaaat tttaatggaa
     2821 accgtatctg acgcactgaa agcactgaaa aaagcctctt cacatgtggt ggcagctcgc
     2881 cttggaatca gtcgtgaaga ggctgtcaac gagctgtggg aactcaaaag aaatggcgtc
     2941 gttgataaaa ctggtcacac ctggtttctg gctggcgaag gtgaatcccg ggtaaccgaa
     3001 gagcggccag taaaatctga agcacaggat atgctgaccg gggaggtcga acaaaaagtt
     3061 accgcagaca tgatgattga gtttatcggt caggatgggg ctaaaacgtg tgaggaactg
     3121 gcggataagt tcggtgttag cattcgcaag gttgcttcca cgttggcggt cgtaacagca
     3181 acggggcgcc tggcacgcgt aaatcagaac ggtaaatttc gttactgcat gccggggggt
     3241 aatttaccag cagatccgaa agccgcgccg gtaacggaaa atgatggtaa ggcctttcct
     3301 cagccagcag gtgctgcgtt accagtccgg gaagccgcaa cacaggaaga aattaaaaca
     3361 gaaactgtgg cggacattgt gcagccgttg ccatcgttta ccgaaacgca agcagatgag
     3421 ctgatttttc cgtcccttcg cagggcaaac ctggcgctgc gcagggcaaa aggtcaggtt
     3481 cagaagtggg agcgtgtctg cgccgcgctg cgggagctga acaagcaccg ggatattgtt
     3541 cggcagattg tcgattcatc cggtcgtatt gtgtcggaaa agtgattgcc ggaggcgctt
     3601 atggcgaaac cttttacaca cgaacagcgt gaagaactga aggcccgaat tatcgggctg
     3661 gtacgcaaaa atgaacgcat gacgatatca caactggaga gagcgacggg agcaggctgg
     3721 cattcagtca gacgttgcct tgtggatgta ctggcttgtg gcgatttata catgcccggt
     3781 aaatacggtg tttttacatc agaacaggtg tatcgcgtat ggcgtaaggc agcggagaaa
     3841 acaaccgacc agacattgat tcgaaagtta ccagacggag aaatacgccg ctacgacaga
     3901 caacagaaca taatctgtgg cgagtgccgg aagagtgaag ttatgctgcg tgtactggct
     3961 ttctatcagg gtaattttca ggaggcgata ctgtgaatga aattagctat caggcttcaa
     4021 ttaccgctgg cattcacatc aaaggagaag agcatggaaa taaaaccaga agatgagtta
     4081 agcaatatcg ttttatttcc ggtaaaagag gatgacccac gtaatcaggt taattttctt
     4141 tatgaggcat cggaaagagc atattgtcat cacgcctctg ttcgggttga cgaaaaagag
     4201 cgtcaggtcc gctgtaaaat ctgcggtgca gttgtggagc cgtttgactg gatgctctct
     4261 gtggcgaaaa gagaaaccag actggcagat gatgtaaggc tcttgcgcca ggaggagcgg
     4321 gaaaggcgaa aaaatataga aaagctaatt cagattgagc gtaacgcgaa agcgcggata
     4381 cgcagggcga caaaatccag aactgaataa ttaaatttag ctctgttaaa aatttaatcc
     4441 ttaaccggag ggatttctgc accctaagaa catcaggagg ccgcccgaaa gggcggtagt
     4501 taaatgcgaa agtttaaaat aattattgaa acgggaatag ccggtggaga tttcgaggat
     4561 gaattcgaag tggatgatga tgcgacgcct gatgaaatac atgacgaagc aaaagatatt
     4621 ttctttaact actgcaatta ctcatatcac gaaataaaag acgaagagga ggaataaaat
     4681 ggctgatttt ggttcaacta aatacagcgt cagttttgaa gaatggcatg aactgttaat
     4741 ggactatgca gagttacgtg gtggcagtgc tgctgatgct gaagcatggc gtgatgatta
     4801 tgaagcaggg aaaactgcgg tcgaagcata ttgtgatgag tggggcgatg aatgagcgag
     4861 attgactatc aggcgctgcg tgaagcagca gagaaagcaa ctaaaggatg ctacatcgta
     4921 gggcatacat cgggcaatca gcatgggaat ataacaggag tttttgtttg tcaaaaatgg
     4981 aaaggagaac ccggtggcgt gattgcagaa tgtcatgtta actgcctggt tgaaacagat
     5041 gctcaggctt acgcaaacgc tgaatttatt gctgccttta atccaaatgt tgcgctggcg
     5101 cttctggatg aacgggaaag aaaccagcaa tacatcaaac gccgcgacca ggagaacgag
     5161 gatattgcgc taacggtagg gaagctgcgc gttgagcttg aagcagcaaa atcaaaactc
     5221 aacgagcagc gtgaa
//
LOCUS       DBFIFI010000257         5202 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.257, whole genome shotgun sequence.
ACCESSION   DBFIFI010000257 DBFIFI010000000
VERSION     DBFIFI010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5202
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.257"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            275..1456
                     /locus_tag="N7B39_004411"
     CDS             275..1456
                     /locus_tag="N7B39_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU2424266.1"
                     /translation="MALTDVKVKTAKPKERPYKLADGGGMYLLINANGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKILAAGGDPGEAKKEEKIALQMSLKNTFEA
                     VAREWHQTKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMELLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLTD
                     LAGYTGSIITKTATQIIMLTGVRTQELRFAHWEDIDFEAKLWEIPAEVMKMKRPHIVP
                     PSEQVIALFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHAQYLDGRREMMQWYADYIDSLSEL
                     A"
     gene            1555..2771
                     /locus_tag="N7B39_004412"
     CDS             join(1555..1860,1863..2771)
                     /locus_tag="N7B39_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611429.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEc31 family transposase"
                     /protein_id="HGU2424267.1"
                     /translation="MISSPQHKTGDLMNKKTKRTFTPEFRLECAQLIVDKGYSYRQAS
                     EAMNVGSTTLESWVRQLRRERQGIAPSATPITPDQQRIRELEKQVRRLEEHNTILKKA
                     NRALDVRLAERFTIVARLSDSHSVVSLCSALEIHRSSYQYWRKRRDTVNPARVRLCSE
                     IRRAWNQSRGSAGARTLAEMLTQNGVPMSRYRAGRLMKYLNLSSCQPGKHQYKNARQE
                     HTCLPNLLERQFAVPEPDRVWCGDITYIWAGNRWCYLAVVMDLFARRVIGWSLSANAD
                     TALISSALRMAYEVRGQPRDVMFHSDQGSQYTGLKYQQLLWRYRIKQSVSRRGNCWDN
                     SPMERFFRSLKTEWVPTDGYTGKDVARQQISSYILNYYNSVRPHHYNGGLTPEESENR
                     YHFYCKTVASIT"
     gene            2780..2932
                     /locus_tag="N7B39_004413"
                     /pseudo
     CDS             2780..2932
                     /locus_tag="N7B39_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0051495 - positive regulation of
                     cytoskeleton organization [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
     gene            3022..3396
                     /locus_tag="N7B39_004414"
     CDS             3022..3396
                     /locus_tag="N7B39_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HGU2424268.1"
                     /translation="MKTLPDTHVREASRCPSPITIWQTLLGRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYVLVRTDQPGFSACTRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKHPEAK"
     gene            3393..3884
                     /locus_tag="N7B39_004415"
     CDS             3393..3884
                     /locus_tag="N7B39_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312567.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HGU2424269.1"
                     /translation="MMKLALTLEADSVNVQALNMGRIVVDIDGVNLAELINMVCDNGY
                     SLRVVDESDRTSADCTPPFTALTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGNGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAGCLPGLPT
                     FNW"
     gene            3896..4093
                     /locus_tag="N7B39_004416"
     CDS             3896..4093
                     /locus_tag="N7B39_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HGU2424270.1"
                     /translation="MTSLTPEAALDILIAWLQDNIDSESGIIFDNDEDKTDSAALLPC
                     IEQVREDVRTLRQLQLLQQNR"
     gene            4178..4519
                     /locus_tag="N7B39_004417"
     CDS             4178..4519
                     /locus_tag="N7B39_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424271.1"
                     /translation="MSGIIISRPEVDTGHTDVICSTSIRHVVTVRNAALQQTETLIRQ
                     LAEISVLTAADIGGKTALDRAMKQDFHCGCWLMEKPETAMKAITRNLDCEIWRDLMQR
                     SGMLSLMDAQG"
     gene            4580..4723
                     /locus_tag="N7B39_004418"
     CDS             4580..4723
                     /locus_tag="N7B39_004418"
                     /inference="COORDINATES: protein motif:HMM:NF025085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424272.1"
                     /translation="MNAFKQLHQSKDEVFERGVINVFRGPNGITKPIPPVNSAVKLSS
                     TTW"
     gene            4717..5136
                     /locus_tag="N7B39_004419"
                     /pseudo
     CDS             4717..5136
                     /locus_tag="N7B39_004419"
                     /inference="COORDINATES: protein motif:HMM:NF025085.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
BASE COUNT         1413 a         1297 c         1295 g         1197 t
ORIGIN      
        1 cggtgaggcg gctggacttc aaatccagtt ggggccgcca gcggtcccgg gcaggttcga
       61 ctcctgtgat cttccgccag tttgcctctc ctgaactctc ccgacatcaa caaagcccct
      121 tataatccct tatatatcag gttttctttg tatcctgagg tcaaacgact ttaacccata
      181 tcaagttgtt tgtgggggtg cattgggggt atcaggttgg ttactggcgg aaattccccc
      241 aggaggatgg attcatcaaa agctggagga tgtcatggcg ctaaccgatg ttaaagtgaa
      301 aaccgcgaag ccaaaagaaa ggccctataa gctggctgat ggcggaggta tgtatctgct
      361 gattaacgct aatggttcca aatactggcg tatgaagtac cgcttcgccg gtaaagaaaa
      421 gatgctgtct attggcgtat accccgatgt aacgcttgcc gacgcacgtg agaaacgcag
      481 tgaagccagg aaaatacttg cggctggtgg cgatccgggt gaggcaaaaa aagaagaaaa
      541 aatagcgctg caaatgagtt tgaaaaacac tttcgaggcc gttgctcgcg agtggcatca
      601 gacgaaagcc gatcgctggt cactgcgcta ccgcgatgaa ataatcgaca cctttgagaa
      661 agatatcttc ccctacatcg gcaagcgacc tattgccgaa atcaagccga tggagctatt
      721 ggaagcacta cgtaagatgg agaaacgtgg agcactggag aaaatgcgca aggtccgcca
      781 acgttgtggt gaggtgtttc gctatgcaat tgttactggt cgagccgact ataaccctgc
      841 tcccgatctt gccagtgctt tagctacacc gaaaaaagta cattttccct ttcttactgc
      901 caatgaactt ccccacttcc ttactgatct ggcgggttat accggaagta tcatcactaa
      961 gacagctact cagatcatta tgttaaccgg tgtacgaacg caagagttgc gtttcgcgca
     1021 ttgggaggat attgattttg aggcaaaatt atgggagatc ccggcagaag taatgaagat
     1081 gaaacggcct catattgtgc cgccctctga gcaggttatt gcgctattta agcaacttga
     1141 accaatctca aaacatcatc ctctggtctt tatcggtagg aacgatcctc gcaagccaat
     1201 tagtaaggag agcattaacc aggtcattga attgctgggg tataagggaa gactcacagg
     1261 gcacggtttc aggcatacca tgagcacaat tctgcacgaa caaggcttta attctgcctg
     1321 gattgaaatg cagttggctc atgtggataa aaactccatc aggggtacct ataaccacgc
     1381 ccagtatctc gatggtcgcc gtgaaatgat gcaatggtac gctgactaca ttgattcact
     1441 ttctgagctg gcctgatgaa tgaaagttcg gtgacacaaa aataatgaat aaccatccag
     1501 ccccaacagg cggatggttg tagtggcaca gtaaatttgg ccacctgatt aaaggtgata
     1561 tcctcaccac aacacaaaac aggtgactta atgaacaaga aaaccaaacg tactttcacc
     1621 cctgaattca ggctggaatg tgcacagcta attgttgata agggctactc atatcgacaa
     1681 gccagtgaag cgatgaatgt cggttctacc acgcttgaga gttgggtgcg ccagctcagg
     1741 cgagagcgtc aggggattgc gccctctgcc acacctatta ctccagacca gcaacgtatc
     1801 cgcgaactgg aaaagcaggt tcgccgcctg gaggaacaca atacgatatt aaaaaaggct
     1861 acaaccgcgc tcttgatgtc cgactcgctg aacggttcac gatagttgcc agactgagtg
     1921 acagccactc agttgtcagc ctctgttctg ctctggaaat acaccgcagc agttaccagt
     1981 actggcgaaa acgacgcgat acggttaatc cggcgcgagt caggttgtgc agcgaaatac
     2041 gccgggcgtg gaaccaaagt aggggctctg cgggggcgcg cacgctggct gaaatgctga
     2101 cccaaaacgg cgtcccgatg agccgttacc gtgccgggcg tctgatgaaa tatctgaacc
     2161 tgagcagttg tcagcccgga aaacatcagt acaaaaatgc tcgtcaggaa catacctgcc
     2221 tgccgaatct gcttgagcgc cagttcgctg tgcccgagcc agatagggta tggtgcggag
     2281 atattacgta tatctgggca ggaaatcgct ggtgctatct ggcggtcgtc atggatcttt
     2341 ttgcccgcag ggttatcggc tggagcctgt cagcgaatgc cgatactgcc ctgataagca
     2401 gtgccctgcg gatggcgtat gaggtgcgtg gtcagccgcg ggacgtcatg ttccatagcg
     2461 accagggaag tcaatataca ggactgaaat atcaacaact tctctggcgt tacaggataa
     2521 agcaaagtgt cagccgacgg ggaaactgct gggataacag tccaatggaa cgcttcttcc
     2581 gcagtctgaa aacagaatgg gtgccaaccg atggctacac aggcaaagac gtggcccggc
     2641 agcaaatcag cagttatatt ctgaattatt acaatagcgt caggcctcac cattataacg
     2701 gagggctgac gccggaagag tcagagaaca gatatcattt ttactgtaaa accgtggcca
     2761 gtattacttg accactacac tggaactcat gctcaccagc ggtgagctga atccccgcca
     2821 tcagcatacc gtcacgctgt atgcaaaagg gctgacctgc aaagccgata ccctcggcag
     2881 tcgaggttac gtttatatgg ctgtttatcc gacgcccgaa acgaaaaagt aaatcatcag
     2941 aataaccttc tgctccggcc tggtgttttc accacgccac ttccccattt ctcatctctg
     3001 catatcagga aaaactttag tatgaaaaca ttacccgata cacacgtacg ggaggcatcg
     3061 cgctgcccgt ctcccatcac catctggcag acactgcttg gccgtctgct ggaccagcac
     3121 tacggcctca cgctgaatga cacaccgttc gctgatgaac gtgtgattga gcagcatatt
     3181 gaggcaggta tttcactgtg tgatgcggtg aactttctcg tggaaaaata cgtgctggtg
     3241 cgtaccgacc agccgggatt cagcgcctgt acccgctctc agttaataaa cagcatcgat
     3301 atcctccggg ctcgcagggc gaccggcctg atgacccgcg acaattacag aacggtaaat
     3361 aacattaccc tgggtaagca tccggaggcg aaatgatgaa actggccctg acactggaag
     3421 ccgacagcgt taacgtacag gcactgaata tggggcgaat tgtcgttgac attgatggtg
     3481 ttaatctcgc tgaactgatt aacatggtct gcgataacgg ctactccctt cgtgttgttg
     3541 atgaatctga ccggacctca gcagactgca cgccaccatt tactgccctt accggcatac
     3601 gctgcagtac cgcacatatc acggaaacgg acaacgcctg gctgtactcg ctgtcacacc
     3661 agaccagtga cttcggtgaa tcagaatgga ttcattttac cggtaacggc tatctgttgc
     3721 gtaccgatgc gtggtcgtac ccggttctgc ggcttaaacg cctgggtctg tcaaaaacgt
     3781 tccgtcgtct ggtcgtcaca ctcatccggc gttatggtgt cagtctcatt catctggatg
     3841 ccagtgccgg atgtctgcca ggtttaccca cttttaattg gtaactagga acaacatgac
     3901 atcattaacc ccggaagcag cactggatat tctgattgcg tggctgcagg acaatatcga
     3961 cagcgaatcc ggaattatct tcgacaacga tgaggataaa acggattcgg cagcattgtt
     4021 gccctgtatc gaacaggtca gggaagatgt ccgtaccctg cgccaactgc agcttctgca
     4081 acagaaccgg tgagtctcac tcaccatact tcattccact gacgccagcc tgaacagggc
     4141 tggcgttttc gtttataaac caaaaggaaa accgataatg tcaggcatca taatctcccg
     4201 tccggaagtg gacaccgggc atacggacgt tatctgctcc accagtatcc gacacgttgt
     4261 gacggtacgg aatgcagcac tgcaacaaac cgaaacgctt atccggcaac tggcagaaat
     4321 ctcagtcctg acagcagcag acattggcgg caaaaccgcc ctggaccggg cgatgaaaca
     4381 ggattttcac tgcggctgct ggctgatgga gaaacctgaa accgcaatga aagccatcac
     4441 ccgcaacctc gactgcgaaa tctggcgtga cctgatgcaa cgctccggga tgctgagtct
     4501 gatggatgca cagggctagg atacatggta ccgctcactg gagtacgata atattccgga
     4561 aatcagcgag gcgaatatca tgaatgcatt caaacaactg caccagagta aggatgaagt
     4621 gtttgagcgt ggggtgatca acgtcttcag agggccgaac ggaattacaa aaccaattcc
     4681 ccctgtaaat tcggcagtaa aattatcgtc aacaacttgg tgagatggga cagatggaga
     4741 tttcatctta tcagtggaat gcaggcagat cgcttagctg acctggaaag aatgttgcat
     4801 ctgctcagcg gtaaaccgct ccccaacaac cgagggaata tcaccattaa tctggatgac
     4861 cacctacagt ccgttcaggg taaagagcgc tatgaagaca agtaaatact ttaagaaggg
     4921 cccgcacaca tcacattcag aaagccagag ctggttgaca ggcttaatga tattattgcc
     4981 agacactacc taggaatgtt accttcacaa aaatctctaa gaattgattc aattgtgtat
     5041 tttagcctca ttccctcatg taaagtcatt tgggggttat tatgggggtc aatcatttat
     5101 ccaacacgca ttaatcattt aaaatcaatg cattaataat catattcgac tcctgtgatc
     5161 tccgccaaac ttcctcttct gaactctccc gacgtcaaca aa
//
LOCUS       DBFIFI010000258         5190 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.258, whole genome shotgun sequence.
ACCESSION   DBFIFI010000258 DBFIFI010000000
VERSION     DBFIFI010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5190
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.258"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(160..2151)
                     /gene="tkt"
                     /locus_tag="N7B39_004420"
     CDS             complement(160..2151)
                     /gene="tkt"
                     /locus_tag="N7B39_004420"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HGU2424273.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIIGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            2429..3187
                     /gene="loiP"
                     /locus_tag="N7B39_004421"
                     /gene_synonym="yggG"
     CDS             2429..3187
                     /gene="loiP"
                     /locus_tag="N7B39_004421"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HGU2424274.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            complement(3393..4313)
                     /gene="speB"
                     /locus_tag="N7B39_004422"
     CDS             complement(3393..4313)
                     /gene="speB"
                     /locus_tag="N7B39_004422"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HGU2424275.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(4449..5180)
                     /locus_tag="N7B39_004423"
     CDS             complement(4449..5180)
                     /locus_tag="N7B39_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGU2424276.1"
                     /translation="MKKWKVRSALVALIVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     TVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGQAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
BASE COUNT         1240 a         1394 c         1363 g         1193 t
ORIGIN      
        1 atgtcaacac gcatatcgtg atgcatatta cataattgat atttattgca tataaatatt
       61 tgcgatctac aacacgcttt atctattgct tgtccgcaaa cggacatatc aaggtaataa
      121 aaaaggtcgc cgaagcgacc tttttacccg aaatgctagt tacagcagtt cttttgcttt
      181 cgcaacgacg ttgtcgacgg tgaagccgaa ctcttcgaac agcaactctg ccggagctga
      241 ctcaccgaag gtggtcatac cgatgatagc gccattcagg ccaacatact tgtaccagta
      301 gtcagcaata cccgcttcta ccgcaacgcg tgcagtaacc gctttcggca gtacggattc
      361 acggtaagcc gcatcctgct tgtcgaatgc atcggtagac ggcatggaaa ccacgcgcgc
      421 cttcacgcct tcggcagtca gtttttcgta ggcggcaaca gccagttcaa cttccgaacc
      481 ggtcgcgatg aagatcagtt ccggctgacc agcacagtct ttcagcacat aaccaccgcg
      541 cgcgatgttt gccagttgct cttcagtacg ttcctgctgc gccaggttct gacgggagag
      601 gataagcgcg gtcggaccgt cctgacgctc aacaccgtat ttccacgcga ccgcggattc
      661 aacctggtca cacggacgcc atgtagacat gttcggggtc acgcgcagag aagcaacctg
      721 ctcaaccggc tggtgagtcg ggccatcttc gcccagaccg atggagtcgt gggtgtaaac
      781 cataacctga cgctgtttca tcagcgcagc catacgtacg gcgttacgtg cgtattcgac
      841 aaacatcagg aaggtggagg tgtacggcag gaagccaccg tgcagggaga taccgttagc
      901 aatcgcggtc ataccgaact cgcgaacacc gtagtggatg tagttacccg cagcatcttc
      961 gttgattgct ttagaaccag accacagggt caggttagac ggtgccaggt cagcggagcc
     1021 gccgaggaat tccggcaaca gcgggccgaa cgcttcgata gcattctgag acgctttacg
     1081 gctggcgatt ttcgccggat tagcctgcag tttagcgata aactctttcg ctttggcgtc
     1141 gaagtcagac ggcatttcgc ctttcatacg gcgggtaaat tcagccgctt cctgcggata
     1201 agctttcgcg taagccgcaa acttctcatt ccatgcagat tctttcgcct ggcctgcttc
     1261 tttcgcatcc cactgcgcat agatttcaga cgggatttcg aacggtgcgt atttccagcc
     1321 cagctgttcg cgggtcagag cgatttctgc atcacccagc ggcgcaccgt gggagtcgtg
     1381 agtaccggct ttgttcgggg aaccgaaacc gatgatggtt ttgcacatca gcagggacgg
     1441 tttgtcagtc actgcgcgcg cttcttctac tgcgcgtttg atggatgctg cgtcatgacc
     1501 gtcgatgtcg cgaataacgt gccagccgta agcttcgaaa cgcattgcgg tgtcgtcggt
     1561 gaaccagcct tcaacgtgac catcgataga aataccgttg tcatcgtaga acgcaatcag
     1621 tttacccagc ttcagcgtac ccgccagaga gcaaacttcg tgggagatgc cttccatcat
     1681 gcagccgtcg cccatgaagg cgtaggtgta gtggtcgaca atgtcgtggc ccggacggtt
     1741 aaactgcgcc gccagcgttt tttctgcaat cgccatacct actgcgttgg caataccctg
     1801 ccccagcgga ccggtggtgg tttccacacc agcggtgtaa cccacttccg ggtggcccgg
     1861 agttttagag tgcagctgac ggaagttttt cagttcttcc atcggcagat cgtaaccggt
     1921 gaggtgcagc aggctgtaga tcagcatgga gccgtggccg ttggacagca cgaagcggtc
     1981 acggtcagcc caggacggat tctgcgggtt atgtttcagg aaatcacgcc acaggacttc
     2041 ggcaatgtca gccataccca taggggcacc cgggtgaccg gatttggctt tctgtactgc
     2101 gtccatgctc agcgcacgaa tagcattggc aagctcttta cgtgaggaca ttttgactcc
     2161 agatcggatg atgaagggca cgcccttaac gacttgacga cagcgcgttt tgggctacgc
     2221 cggaaaattt gccaacaatt taccgcaagc cgcgcgtcat gtacatggaa catccttttg
     2281 ccgcttcaga aatctctgga tcatgctcgc atgttgcgca atctactcgc ccgtccgctg
     2341 cgcttttcct tatactgaga ctgagcgtcg gttcacctgc aaacggcgca tttttagaat
     2401 aatcctgacc ttgtgcggaa gaaaaaacat gaaaattcgc gccttattgg tagcaatgag
     2461 cgtggcaacg gtactgaccg gttgccagaa tatggactcc aacggactgc tctcatcagg
     2521 agcggaagct tttcaggctt acagtttgag tgatgcgcag gtgaaaaccc tgagcgatca
     2581 ggcatgtcag gagatggaca gcaaggcgac gattgcgcca gccaatagcg aatacgctaa
     2641 acgtctgaca actattgcca atgcgctagg caacaatatc aacggtcagc cggtaaatta
     2701 caaagtgtat atggcgaagg atgtgaacgc ctttgcaatg gctaacggct gtatccgtgt
     2761 ctatagcggg ctgatggata tgatgacgga taacgaagtc gaagcggtga tcggtcacga
     2821 aatggggcac gtggcattag gccatgtgaa aaaaggaatg caggtggcac ttggtacaaa
     2881 tgccgtacga gtagctgcgg cctcggcggg cgggattgtc ggcagtttat ctcaatcaca
     2941 actcggtgat ctgggcgaga aattagttaa ttcgcaattc tcccagcgcc aggaagcaga
     3001 agccgatgat tattcttacg atcttctgcg ccaacgcggc atcagcccgg caggtcttgc
     3061 caccagcttt gaaaaactgg caaaactgga agaaggtcgc caaagctcaa tgtttgacga
     3121 ccatcctgca tccgccgaac gcgcccagca tattcgcgat cgcatgagcg cggatgggat
     3181 taagtaaagc ctggtggtgt cgaacgtagg tcagataagg cgttcacgcc gcatctgaca
     3241 tgaatggcac atttgttacc ttgtgcgcat tgccggatgc gatgctggcg catcttatcc
     3301 ggcctacggg tgccgaacgt aggtcggata aggcgttcac gccgcatccg acattaatgg
     3361 cacgttttac ccgtgcgcat cgcatctggt gcttactcgc cctttttcgc cgcctgaata
     3421 tacagcattt ccagcgccag cgttgccgct gccagagcgg tgatttccga ctgatcgtat
     3481 gccggagcca cttccactac gtccatccca acgatgttga gatctttcag gccgcgtacc
     3541 agtttaatag cgcgatcgga ggtcagaccg ccaattaccg gcgtaccggt tcccggcgca
     3601 aatgcaggat ccaggcagtc gatatcaaag gtcaggtaaa ccggcatatc acccacaatt
     3661 tgtttcacct gggcgataac gtcatccacg ctgcgatcgt tcacctggca ggcgtccagc
     3721 acggtaaagc cgttgtcttt atcaaattcg gtacgaatac caatctgcac ggaatgattc
     3781 gggtcgatca gaccttcttt cggcgcggta tagaacatgg tgccgtggtc aaattcacaa
     3841 ccgttcgcgt aggtatcggt gtgggcgtca aagtgtacca gcgccatttt gccgaaatgc
     3901 ttcgcatgag cacgcagcag cggcagcgta acaaagtggt caccaccgaa ggagagcata
     3961 cgcttaccgg cagccagcag cttctcggcg tgcgcctgca gcttctcgct catctcacgg
     4021 gcatcgccga aagcatatac cagatcacca cagtccacga cgttcagacg ctcacgcatg
     4081 tcgaaattcc acgggaagcg gttgtgttcc caggccagat tcgtcgaaac ctgacggata
     4141 gctgccggac cgtggcgtcc ccccgcacga ccagaagtgg ccatatcgaa cggcacacca
     4201 gtaatcaccc agtcagcatc gctgtcatac ggctggaagt tcatcggcag gcgcaaaaaa
     4261 ccaaaggcgt tggaaaccag tgagttatcg tattgatgac ctaaggtgct catggatgga
     4321 ctcctattac aaagtcgata taaaaaaacc cttccgcgtc gttaggcccg acgaggaagg
     4381 gttggatttg cgaaacacaa gtttgtgcta attatcgcct taaatgggcg aggcatcaag
     4441 cgaggttgct atttccgaca cggtggcggc gcatagccat tgttatctgc cggaaaggct
     4501 tgtccagagc acggtggcag cgcgttttgc gcctggcctt gtaattcaaa ggcggcaaag
     4561 ccgattacac cggtattacg cacatcgcct tgcgccgaat tggctgcgta ggaatcagca
     4621 acgttggcga actgaaaagc cgcttcggta tgtttatctt tgcggaatcc tttaatcgct
     4681 aacgaatctc cggcattcac aatatagccg ttgttattca gcgaaccttg cttgccgttc
     4741 agcacatcca ggccatcaac cgtagcaacg atctcgtagt tggtgttacg actgtaatta
     4801 cggacatata attgataatt cattccgact ctggcatgga caatatagcg tggttcatta
     4861 ttagatgaat cataaacacg cgtaataggt agagaattaa aattaccatc gcgaatagca
     4921 tattccagtt cgttaatacg aatgctataa acgcgatcgt agccggaagg aacattagtc
     4981 gaataactaa taacgatcat ctccgccgga tcgcgccagg ctcgctcagc agatacgccc
     5041 tgaaccgttg agtgcacatc cccaccccag gcggtgccga cattgcccga tgcactgcta
     5101 ttatactgcg cattactgga acaaccggcc agcagcacga ttaatgccac caacgcgctg
     5161 cgtactttcc attttttcat aaccgttccc
//
LOCUS       DBFIFI010000259         5184 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.259, whole genome shotgun sequence.
ACCESSION   DBFIFI010000259 DBFIFI010000000
VERSION     DBFIFI010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5184
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.259"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..3709
                     /locus_tag="N7B39_004424"
     CDS             <1..3709
                     /locus_tag="N7B39_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HGU2424277.1"
                     /translation="LVIKNGTVYNASGVPVVDINKPNGSGLSHNIWDNLNVDKNGVVF
                     NNSANESSTSLAGNIQGNSNLTSGSAKVILNEVTSKNPSTINGMMEVAGDKADLIIAN
                     PNGITVNGGGSINTGKLTLTTGTPDIQDDKLAGYSVNGGTITLGKLDNASPTEILSRN
                     VVVNGKVSADELNVVAGNNYVNAAGQVTGSVSATGSRNGYSVDVAKLGGMYANKISLV
                     STEKGVGVRNLGVIAGGVNGVSIDSKGNLLNSNAQIQSASTINLTTNGTLDNTTGTVT
                     SVGTISLNTNKNTIVNTRAGNISTMGDIYVNSGTIDNTNGKLAAAGMLAVDTNNATLI
                     NSGKGSSVGIEAGLVALKTGTLNNSNGQIRGGYVGLESAALNNNNGDIQTTGDIAIIS
                     NGNVDNNKGLIRSSTGHIVIGAAGSVNNGSTKTADTGSSDSLGIIADTGVEIGANNIN
                     NNGGQIASNGNVSLSSYSTIDDYAGKILSNSKVIIKGSSLRNDTGGISGKQGIEVAVG
                     GSLTNNIGVISSEEGDISLLANSVDNHGGFMMGQNITMESMSGVNNNTALIVASKKLK
                     INARGSIENRDGNNFGNAYGLYFGMPQQTGGMVGKEGIELSGQNIYNNNSRLIAEDGP
                     LTLQAQNTFDNTRALVTSGADASIQVGGTYYNNYATTWSAGNLDIDATTLQNSSSGTM
                     IDNNATGFIASDKNLSLEVVNSLTNYGWISGKGDVDVTVNNGNLYNRNTIAAEKGLDI
                     AALNGIENWKDISAGGDLTMNTNRHVTNNSNSNMVGQNIVINAVNDINNRGNIVSDAD
                     LNVTTKGNLYNYLYMVGYGDIALSANSVANNNATIEATGDLIIDSKGNVGNNRGNLHA
                     LNGVLSVKGNNLNNDNGEIRGYGDVTLALTGNYDSYKGSLTSETGDVTLTANIVDNAY
                     GLIAGENVSVDAKSTIYNNTALIAANKKLVINAGGNLENRDGNNFLRNNGALFGITDN
                     VGGIVGKEGVTLSAQNVYNNNSSIIAENGPLNLLSRGTLDNTRALLSSGADAIIRAAG
                     TFYNNYATTYSAGNLDVYAASLNNASDGRLEDNTATGVIASDKNLDLSVDNSVTNYGW
                     ISGKGDVHFNVLKGTLYNRNAIAADNALTINALNGVENFKDIVAGTALTIDTQKYVTN
                     NSNSNMLGQTIAINAVNDINNRGNIVGDYSLGVKTTGNIYNYLNMLSYGVAGVSANKV
                     TNSGKDAVLGGFYGLALEANETDNTGTIVGM"
     gene            3798..>5184
                     /locus_tag="N7B39_004425"
     CDS             3798..>5184
                     /locus_tag="N7B39_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HGU2424278.1"
                     /translation="MQLRFNFGLLLILCCFQVAAKDSLDNLIKQQKNADESTLRAHKL
                     EKKDVYSNAQRNAFTLGDLPQEKNCFIINRIELEDNFLGGQLISEVQKAIAGRCVGSA
                     GVQKIAAALQDYYINAGYITTRISIPSQDISKGKLRFQVNAGKIEKTIVEGNDISEWM
                     LPFQRNDILNIRDIEQGLENLQRVPDVDVKINIEPGTRDGYSIVHIDTHRGKNWSVRA
                     SYNNWGDEETGRYQTTSVGYLFNPAKMGDLFYLAGTRSTTGQYENVSSYYSIPVGYWE
                     YGFFYSKSKSQQVIPLSYISLDYVGISEYLSAKATHTVYRDKTKKFAGSAELIRRKSN
                     YTLNGEELTLQARDMGNIKLGLNYKQQLPGAFWDSTLSWQRFLTWFGGEKTPDMQYGD
                     VSPVSNIFNFEGNYTRQIENGYYNTAFFAQYAPRELTLQDKITVGDRWSIRGFENSVG
                     LSGNDGFYIKNT"
BASE COUNT         1471 a         1111 c         1354 g         1248 t
ORIGIN      
        1 attagtcata aaaaatggaa cggtatataa cgccagtggt gtgccagtcg ttgacatcaa
       61 caaacctaac ggtagcggtt tatctcataa tatctgggat aacctaaacg ttgataaaaa
      121 tggtgtcgtt ttcaataata gcgctaatga atccagtact tcacttgccg gaaatattca
      181 gggaaacagt aatctgacct ccgggtcggc gaaggtgatc ctgaatgagg ttacttccaa
      241 aaatccttca accattaatg ggatgatgga agttgcaggg gataaagcgg atctgattat
      301 tgccaacccg aatggtatta ctgtaaacgg tggcggttca atcaatacag gtaaacttac
      361 cttaaccacc gggacgccgg atatccagga tgacaagctg gccggttact ccgtgaacgg
      421 cggtaccatt acgctcggta aactggataa cgccagcccg acagaaattc tgtcccgtaa
      481 cgtggtagtt aacggcaaag tgtctgccga tgagctgaac gttgttgctg gcaataacta
      541 tgttaatgcc gcaggccagg tgaccggtag cgtatccgcc acggggtccc gtaacggtta
      601 cagcgtagat gttgccaaac tgggcggaat gtatgcgaac aaaatcagtc tggtcagcac
      661 cgagaaaggt gtgggggttc gcaacctcgg cgttattgct gggggtgtta atggtgtcag
      721 catcgattcc aaaggtaacc tgttaaacag taacgcccag attcagtctg caagcacgat
      781 caacctgaca acaaatggta ctctggataa caccaccggt acggtgacat ctgtaggcac
      841 tatctcgctt aataccaaca agaatactat cgtgaatacc cgtgcgggta acatctctac
      901 gatgggcgat atctacgtta acagcggtac gattgacaat actaacggca agcttgcggc
      961 tgcaggaatg ctggcggttg ataccaataa cgccacgctg attaactctg gtaaagggag
     1021 ttctgtcggg attgaagcgg ggctcgtggc gctgaaaacc ggaacgctca acaacagcaa
     1081 tggtcagatt cgcggtggct atgtgggtct tgaatccgct gcgctgaata acaacaacgg
     1141 tgatatccag accaccggcg atatcgccat tatcagtaac ggtaatgtgg ataacaacaa
     1201 aggtctgatc cgttcgtcca ccgggcatat cgttattggc gcggcaggta gcgtaaataa
     1261 tggttcaacc aaaaccgccg ataccggcag ttctgactct ctgggcatta ttgcagatac
     1321 cggcgtagaa attggtgcga acaacatcaa taacaacggc ggacagattg cgtctaatgg
     1381 caacgtctcc ctgtcaagtt acagcacgat cgacgactat gcgggcaaaa ttctgtccaa
     1441 cagcaaagtg attatcaagg gaagctctct gcgtaacgat accgggggga tcagcggtaa
     1501 gcagggtatt gaagtcgccg ttggcggcag cctgaccaat aatattggcg tgatcagctc
     1561 tgaagagggt gatatctccc tgttagccaa ctccgtggat aaccacggcg gcttcatgat
     1621 ggggcagaac atcacgatgg agtcgatgtc tggcgtcaat aacaacacag cgctgatcgt
     1681 ggccagcaaa aaactgaaga taaatgcgcg cggcagtatc gaaaaccgcg atggcaataa
     1741 cttcggtaat gcttatggtc tgtacttcgg catgcctcag caaacgggtg gaatggtcgg
     1801 caaggaaggc atcgagcttt ccgggcagaa catctataac aacaacagcc gtcttatcgc
     1861 tgaggatggt cctctgactc tgcaggcgca gaacacgttc gacaacacgc gtgctctggt
     1921 caccagcggg gcggatgcat ctattcaggt tggcggaacg tattataaca actacgctac
     1981 cacctggagt gcgggcaacc tggatatcga cgcgaccacg ctgcaaaaca gcagcagcgg
     2041 tacgatgatc gataacaatg cgaccgggtt catagcatct gataaaaacc tgtcactgga
     2101 agtggtgaat agccttacca actacggctg gatcagcggt aaaggcgatg ttgatgtcac
     2161 ggtgaataac ggcaacctgt ataaccgcaa taccattgcg gctgaaaagg ggctggatat
     2221 tgccgcgttg aacggtattg aaaactggaa ggatatttct gctggcggcg acctgacgat
     2281 gaacaccaat cgccatgtga ccaacaactc caacagcaat atggtggggc agaatattgt
     2341 tattaacgcg gttaacgata tcaacaaccg tggcaacatt gtcagtgacg ctgacctgaa
     2401 cgtgacgacc aaaggcaacc tgtataacta tctctatatg gtagggtatg gggatatcgc
     2461 attgtcggca aatagcgtgg cgaacaataa cgcgaccatc gaagcgacag gcgatctgat
     2521 tatcgattcg aagggtaacg tgggtaacaa ccgcggtaat ctgcatgcgt tgaacggcgt
     2581 gttgtctgtt aaaggcaaca atctgaacaa cgataacggt gaaattcgtg gttatggcga
     2641 tgtcacgctg gcactgacgg gcaactacga cagctataag ggttcgctga cctctgaaac
     2701 gggcgacgtg actctgacgg cgaacattgt agacaacgcc tatggtttga ttgccggtga
     2761 gaatgtttct gtcgatgcta aatcgacgat ttacaacaac actgcgctga tcgcggcgaa
     2821 taaaaagctg gttattaacg ctggcggcaa cctcgaaaac cgcgacggga ataacttcct
     2881 gcgtaataac ggcgcgctgt ttggaattac cgacaacgtt ggcggcatcg taggtaaaga
     2941 aggtgtcacg ctttctgctc agaacgtcta caacaataac agcagcatca tcgctgaaaa
     3001 tggtccgctt aatctgctgt ccaggggaac gctggataat acccgcgcgc ttcttagcag
     3061 tggggctgat gccatcatcc gtgcggcagg gacgttctac aacaactatg ccaccacgta
     3121 cagcgccggt aatctcgacg tttatgcggc gtcgttgaac aacgccagcg atggtcgcct
     3181 ggaagacaat accgccacgg gcgtgattgc gtctgacaaa aacctggatc tgagcgttga
     3241 taacagtgtc actaactatg gttggatcag cggtaaagga gatgtgcatt tcaatgttct
     3301 gaaaggcacg ctgtataacc gtaatgccat cgcggcggac aacgcgctga ccattaatgc
     3361 cctgaacggt gttgagaact ttaaagacat tgtggcgggt actgcgctga ctattgatac
     3421 gcagaagtat gttaccaaca acagcaacag taatatgttg ggacaaacca tcgcgatcaa
     3481 tgccgtgaat gacattaata accgtggaaa tattgtgggt gattattctc tgggtgttaa
     3541 aaccaccggt aatatttata actacctcaa tatgctgagt tatggtgtcg ctggcgtatc
     3601 ggcaaataag gttacgaata gcggtaaaga cgctgttctc ggtggcttct acggtttagc
     3661 gttagaagca aacgaaactg ataacaccgg tactattgtc ggcatgtaag atgtaaccaa
     3721 agacagcagt ggcttttaat attaatgcgt catgtcacgc aggggttgct gctgtcttta
     3781 tttagtttga gaagataatg caattacgat ttaattttgg tttgctgctc attctttgtt
     3841 gttttcaggt tgcagcgaaa gacagtcttg ataatttgat caagcagcaa aaaaatgcag
     3901 atgaaagcac gttgcgcgcg cataaattag aaaaaaaaga tgtttactca aatgcacaac
     3961 gtaatgcgtt tactctgggt gatctgccgc aggaaaagaa ttgctttatc attaatcgga
     4021 ttgaactgga agataacttt ctgggtggtc agttgatttc tgaagtgcaa aaagcgattg
     4081 ccggacgttg tgtcggtagt gctggtgtgc aaaaaattgc agctgccttg caggattatt
     4141 atattaatgc gggatatatc acaacgcgaa ttagtatccc ttcgcaggat atttctaaag
     4201 gcaagctacg tttccaggtt aatgccggga agatagaaaa aacgattgtc gaaggtaatg
     4261 atatcagtga atggatgtta ccttttcagc gtaacgatat tctcaatatt cgtgatattg
     4321 aacaggggct ggaaaatctt cagcgagtac ctgacgttga tgtgaaaatt aatattgaac
     4381 cgggaacacg ggatggttac agcatcgttc atattgatac acaccggggg aaaaactgga
     4441 gcgttcgggc aagttacaac aattggggcg atgaagaaac gggccgttac cagacaacct
     4501 cggtgggtta tctgtttaat ccagcgaaaa tgggggatct gttttatctc gccggtacgc
     4561 gtagtactac cggacagtat gaaaacgtca gtagttatta ttccattccg gtggggtact
     4621 gggagtacgg ctttttctat agcaaaagta aatcgcagca ggtgattccg ctttcgtaca
     4681 tctcgctgga ttatgttggc atcagcgaat acttgagcgc aaaagcgacg cataccgttt
     4741 atcgcgataa aacaaaaaag ttcgccggta gtgcagaact cattcgacga aagtcgaatt
     4801 acacgcttaa tggtgaggag ctgacattac aggcgcggga tatgggaaat atcaagctgg
     4861 ggctaaatta taagcaacag ttacccggcg cgttctggga ttcaaccctc tcgtggcaac
     4921 gctttttaac ctggttcggt ggtgaaaaaa cgccggatat gcaatatggc gacgttagcc
     4981 cggtaagcaa tatctttaat tttgagggta actatacccg acaaatagag aatggatatt
     5041 acaataccgc tttttttgcc cagtacgcac cgcgtgaact gactttgcaa gataaaatta
     5101 cagttggcga tcgttggagt attcgcgggt ttgaaaatag cgtaggtttg tcagggaatg
     5161 atggttttta tataaaaaat acgc
//
LOCUS       DBFIFI010000260         5139 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.260, whole genome shotgun sequence.
ACCESSION   DBFIFI010000260 DBFIFI010000000
VERSION     DBFIFI010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5139
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.260"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            136..4038
                     /gene="espP"
                     /locus_tag="N7B39_004426"
     CDS             136..4038
                     /gene="espP"
                     /locus_tag="N7B39_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter EspP"
                     /protein_id="HGU2424279.1"
                     /translation="MNKIYSLKYSHITGGLIAVSELSGRVSSRATGKKKHKRILALCF
                     LGLLQSSYSFASQMDISNFYIRDYMDFAQNKGIFQAGATNIEIVKKDGSTLKLPEVPF
                     PDFSPVANKGSTTSIGGAYSITATHNTKNHHSVATQNWGNSTYKQTDWNTSHPDFAVS
                     RLDKFVVETRGATEGADISLSKQQALERYGVNYKGEKKLIAFRAGSGVVSVKKNGRIT
                     PFNEVSYKPEMLNGSFVHIDDWSGWLILTNNQFDEFNNIASQGDSGSALFVYDNQKKK
                     WVVAGTVWGIYNYANGKNHAAYSKWNQTTIDNLKNKYSYNVDMSGAQVATIENGKLTG
                     TGSDTTDIKNKDLIFTGGGDILLKSSFDNGAGGLVFNDKKTYRVNGDDFTFKGAGVDT
                     RNGSTVEWNIRYDNKDNLHKIGDGTLDVRKTQNTNLKTGEGLVILGAEKTFNNIYITS
                     GDGTVRLNAENALSGGEYNGIFFAKNGGTLDLNGYNQSFNKIAATDSGAVITNTSTKK
                     SILSLNNTADYIYHGNINGNLDVLQHHETKKENRRLILDGGVDTTNDISLRNTQLSMQ
                     GHATEHAIYRDGAFSCSLPAPMRFLCGSDYVAGMQNTEADAVKQNGNAYKTNNAVSDL
                     SQPDWETGTFRFGTLHLENSDFSVGRNANVIGDIQASKSNITIGDTTAYIDLHAGKNI
                     TGDGFGFRQNIVRGNSQGETLFTGGITAEDSTIVIKDKAKALFSNYVYLLNTKATIEN
                     GADVTTQSGMFSTSDISISGNLSMTGNPDKDNKFEPSIYLNDASYLLTDDSARLVAKN
                     KASVVGDIHSTKSASIMFGHDESDLSQLSDRTSKGLALGLLGGFDVSYRGSVNAPSAS
                     ATMNNTWWQLTGDSALKTLKSTNSMVYFTDSANNKKFHTLTVDELATSNSAYAMRTNL
                     SESDKLEVKKHLSGENNILLVDFLQKPTPEKQLNIELVSAPKDTNENVFKASKQTIGF
                     SDVTPVITTRETDDKITWSLTGYNTVANKEATRNAAALFSVDYKAFLNEVNNLNKRMG
                     DLRDINGEAGAWARIMSGTGSASGGFSDNYTHVQVGVDKKHELDGLDLFTGFTVTHTD
                     SSASADVFSGKTKSVGAGLYASAMFDSGAYIDLIGKYVHHDNEYTATFAGLGTRDYST
                     HSWYAGAEAGYRYHVTEDAWIEPQAELVYGSVSGKQFAWKDQGMHLSMKDKDYNPLIG
                     RTGVDVGKSFSGKDWKVTARAGLGYQFDLLANGETVLRDASGEKRIKGEKDSRMLMSV
                     GLNAEIRDNVRFGLEFEKSAFGKYNVDNAVNANFRYSF"
     gene            complement(4294..4775)
                     /locus_tag="N7B39_004427"
                     /pseudo
     CDS             complement(4294..4775)
                     /locus_tag="N7B39_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         1551 a         1123 c         1161 g         1304 t
ORIGIN      
        1 tttgttatcc gtatgataac aaacacataa aggagtaaac atttatctgg acgatgatta
       61 caatgttcac gactgtcaga gacacacctc tgatacatta gaaaaaccaa tgtttccctt
      121 aaaaatggag cttatatgaa taaaatatac tctcttaaat acagccatat tacaggaggg
      181 ttaatcgctg tttctgaatt atccggcaga gtatcatcaa gagcaactgg taagaaaaaa
      241 cacaaacgca tacttgcatt atgtttttta ggcttattac aatcctcata ttcttttgcg
      301 tcacagatgg atatttcaaa tttctacatc cgtgactata tggattttgc acagaacaag
      361 ggcatatttc aggctggcgc aacaaatatt gaaatagtga agaaagatgg ctccaccctg
      421 aaactaccgg aagtaccatt tcctgacttc tcaccggttg caaacaaagg gtcaaccaca
      481 tctattggtg gtgcatacag tatcacagcc acacacaata cgaaaaacca ccactcagtt
      541 gcgacgcaaa actgggggaa cagcacgtac aaacaaactg actggaatac ttcacatcct
      601 gattttgcag tatcccgact tgacaagttt gttgttgaga cccgaggtgc gactgaaggc
      661 gcagatattt cgttatcaaa acagcaggca cttgaacgtt acggggttaa ttataaagga
      721 gaaaagaaac ttatcgcatt cagagccggc tctggtgtgg tatccgttaa aaaaaatgga
      781 cgcataactc catttaatga ggtttcttat aagccagaaa tgttaaatgg ctctttcgtt
      841 cacattgatg actggagtgg atggctgata ttaaccaaca accagtttga tgagtttaat
      901 aacattgcct ctcagggtga cagcggttca gcactgttcg tctatgataa ccaaaagaaa
      961 aagtgggttg tcgctggaac tgtctggggg atttataatt acgccaatgg caaaaaccac
     1021 gcagcataca gtaaatggaa ccagacaacc attgacaacc tgaagaacaa gtattcttac
     1081 aacgtggata tgtcaggggc tcaggttgca accattgaaa atggaaaact gacaggcact
     1141 ggctcagaca ccaccgatat aaaaaataag gacttaatat ttactggcgg tggagatatc
     1201 ctcctgaaat cctcttttga taatggtgct ggcggtcttg tctttaatga taaaaagacc
     1261 tatcgagtaa acggggatga tttcaccttt aaaggtgccg gtgttgatac aagaaacggc
     1321 agcaccgttg agtggaatat ccggtatgat aataaagaca accttcacaa aattggtgat
     1381 ggcacattag atgtccgaaa aacccagaac accaacctga aaacaggtga gggtcttgtc
     1441 attcttggag ctgaaaaaac attcaataat atctacataa ccagtggtga tggaactgtc
     1501 cgactgaatg cagaaaatgc actgtctggc ggtgaataca acggtatttt ctttgcgaaa
     1561 aatggcggaa ctcttgacct gaacggatat aatcagtctt tcaataaaat tgctgcaact
     1621 gattcaggtg ctgtaataac caatacgtca accaaaaaat ccattttatc cctgaataat
     1681 actgctgact atatctatca cggcaacata aacgggaatc tggacgtact tcagcatcat
     1741 gagacgaaaa aagagaaccg tcgtcttatt cttgatgggg gcgtggacac aacaaatgat
     1801 ataagcctgc gtaatacaca actgtccatg cagggacatg ccactgaaca tgccatttat
     1861 cgggatggag ctttctcttg ttcactacca gctcctatgc gctttttgtg tggcagtgat
     1921 tatgttgcag gaatgcaaaa tacagaagct gatgctgtaa aacaaaacgg aaatgcctat
     1981 aaaaccaaca atgctgtctc tgatttatcg cagccagact gggaaaccgg aacattcaga
     2041 tttggaacgc tacatcttga aaattccgat ttttctgttg gtcgtaatgc aaatgtaatc
     2101 ggggacattc aggccagtaa atcaaacatt actattggtg acactacagc atatattgat
     2161 ttgcatgctg gtaaaaatat taccggtgat ggttttggct tccgccagaa tattgtgcgt
     2221 ggaaactcac aaggagaaac gctgtttaca ggagggatca cagcagaaga cagcactatc
     2281 gttattaaag ataaagcaaa agcattattt tcaaattatg tatacctgct gaacacaaaa
     2341 gcaaccatag agaacggtgc tgatgtgaca actcaaagtg gtatgttctc cacgagcgat
     2401 atcagcatct ctggtaatct gtccatgaca ggcaatcccg acaaagacaa taaattcgag
     2461 ccctcaatat atctgaatga tgcttcttat ctactgactg acgactccgc cagactcgtt
     2521 gccaaaaata aagcatctgt ggtgggagat atacactcca ctaaaagtgc atccatcatg
     2581 tttggtcatg atgaaagcga cctctcgcag ttgtctgaca gaacctcaaa agggcttgca
     2641 cttggtcttt taggtggctt tgatgtctca tatcgcggtt cagtcaatgc cccgtcagca
     2701 tctgccacta tgaacaacac ctggtggcaa ctaaccggag attctgcgct gaaaacactg
     2761 aaaagtacaa acagcatggt ctatttcact gacagcgcaa acaataagaa attccatacg
     2821 ctgacggtcg atgagctggc aaccagcaac agcgcctatg cgatgcgtac aaacctttct
     2881 gaatcagaca aactggaggt caaaaaacac ttgtctggtg agaacaatat tttactcgtt
     2941 gatttccttc agaaaccaac gcctgaaaaa caactgaata ttgaactggt aagcgcgcca
     3001 aaagacacca atgaaaatgt ctttaaagcc agtaaacaaa ccattggttt cagtgatgta
     3061 acgccggtca ttacaaccag ggaaaccgat gacaaaataa catggtcact gacaggctat
     3121 aacacggtag caaacaagga agcaacccgg aatgccgccg ccctgttctc tgttgactat
     3181 aaagcgtttc tgaacgaggt caacaacctg aacaaacgta tgggtgacct gcgtgatatc
     3241 aacggcgaag ccggtgcatg ggcacgcatc atgagcggta ccggctctgc cagtggtggt
     3301 ttcagtgaca actacacgca cgttcaggtc ggggtcgaca aaaaacacga gctggacgga
     3361 ctggatttgt ttaccggttt cactgtcaca cacactgaca gcagtgcctc cgccgatgtt
     3421 ttcagtggta aaacgaagtc tgtgggggct ggcctgtatg cttccgccat gtttgattcc
     3481 ggtgcctata tcgacctgat tggcaagtat gttcaccatg ataatgagta cactgcaacc
     3541 tttgccggac tcggaacccg tgattacagc acgcattcat ggtatgccgg tgcagaagcg
     3601 ggctaccgct atcatgtcac tgaggatgcc tggattgagc cacaggctga gctggtttac
     3661 ggttctgtat ccggtaaaca gtttgcatgg aaggaccagg gaatgcatct gtccatgaag
     3721 gacaaggact acaatccgct gattggccga acgggtgtgg atgtgggtaa atccttctct
     3781 ggtaaggact ggaaagtgac agcccgtgcc ggtctgggct accagttcga cctgctggct
     3841 aacggcgaaa ccgtattgcg ggatgcatct ggtgaaaaac gcatcaaagg tgaaaaggac
     3901 agccgtatgc tgatgtccgt tggcctgaat gcagaaatca gggataacgt ccgctttgga
     3961 ctggagtttg agaaatccgc ctttggtaag tacaacgttg ataatgctgt caacgctaat
     4021 ttccgttact cgttctgata ctccgtattc aaaagggcct gcgggccctt tacgctcagt
     4081 gcctgccgcc ctgatatgtc tgagagacat ttccgggatg tcagtcgact ggtttactta
     4141 actgcgatga ctaaagctgg cagtaatgcc ggctttttta cggccacata cgtcttcctc
     4201 tcatccggaa ctgtgataca aatcctgcat acaggattga ccgccggttc ctgcaaatgg
     4261 cacattttca gtaagatagc gaaggttaca tttgtcactg gttaacatac cgatggtaaa
     4321 aggggcaagc agcgccagca attcatcagc ccgggggcca aaagtgtagg ccagaacgcc
     4381 accagttttc gtgttgtacg cgtaccagag cctgtgctgt cacgctttac tgccgataaa
     4441 actccattgc tcatcaagtt cgcagatgag tgccatatca gcatgagcga ccggggatga
     4501 cgttattctt cgtggcgagc gttttttaaa gtccgaatga cagtattaat gccgattttc
     4561 agcgtacggg cagtatcgcg aactccggca ccgttgaaag gcatttcagt gatttggtct
     4621 tgaactcccg gtttacgggc ctcgtaacgg taagtgagct gaaacactcg atgacagtca
     4681 cggcagcgaa atcgatcatg tccttttggg ttcttcccgt gacggtaaac ctgaacggac
     4741 tggcaacggg ggcaatgaag gttaactgtg accattaaag aaccttaaaa gtgactatta
     4801 tacccctaat tcaactaatt ggaggcatta ccatgcatta gtgcaagcgc cttgtggatt
     4861 cttttgctaa tggcggaaac atattgtaca atattgcatt tatccctcaa taaataaatg
     4921 agttatcaga ttgcaaaaat ctgttatcaa actaattcaa ataaaaagag attgatacga
     4981 ccaacgtaca caaaaatttt cgatattcga actaaataac agctcctaat accgctacac
     5041 tttagctatc cagtctgcgc ctcagaaatg gacgatgccg ttgaaggact ggtgtatggc
     5101 aatgagccgc tttattatcg aattcggtga ccgcttgga
//
LOCUS       DBFIFI010000261         5110 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.261, whole genome shotgun sequence.
ACCESSION   DBFIFI010000261 DBFIFI010000000
VERSION     DBFIFI010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5110
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.261"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            1303..2019
                     /gene="nanC"
                     /locus_tag="N7B39_004428"
     CDS             1303..2019
                     /gene="nanC"
                     /locus_tag="N7B39_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HGU2424280.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            2039..3145
                     /gene="nanM"
                     /locus_tag="N7B39_004429"
     CDS             2039..3145
                     /gene="nanM"
                     /locus_tag="N7B39_004429"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HGU2424281.1"
                     /translation="MNKTITALAILMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLETQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLISHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAVDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNRLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            3210..4190
                     /locus_tag="N7B39_004430"
     CDS             3210..4190
                     /locus_tag="N7B39_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HGU2424282.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQSYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALVKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEAIYGNYQNNILANIIFVDFQQQGARGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSSAARRGIISDRFVEAILQFWRER"
     gene            4313..5008
                     /locus_tag="N7B39_004431"
                     /pseudo
     CDS             4313..5008
                     /locus_tag="N7B39_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
BASE COUNT         1491 a         1130 c         1140 g         1349 t
ORIGIN      
        1 acagttaatt ataataacgt tgccatacaa acgctatgct cttgcatgct atgtaccttt
       61 atatatttat caatcggata cgaatctaat aataaccctc atcattaatg caactaatct
      121 tatctatata tcatgtgata tgttctgtaa caagtaaaat caacataaaa aattcagcaa
      181 aatttaatat tcgttcaact agccgtctca catatgacat tattagtcaa ctccatttgc
      241 cacaaaatgg ctatttcatg gaacacagta atttcttagt ggctattatc aagctaattc
      301 ttaggtccct agcgattatt cctggctgat tcgtcagctt acgccatgag attgttacat
      361 tcctttttat cctggagata tgggggaacg caggagatga ttttttcatc tacattcgtt
      421 ggtaaatata gctttggtca ctacgactta aaccttcctc aatcacagcc attcgccggg
      481 cgatacggca aacaaactaa gtgaaacatc atcctgaaaa caccaacatc aataagcctc
      541 tccagatcga cttcagaagt gaccagttac aagccacaaa ccaggaaaca tatttcatga
      601 tgagaattat gctcagcctg atggcgggta aacactgatt tacaccaagc tcactgagtt
      661 agcttgcaaa gcttcccttg tttagactct aaataaatta tttataaaac aaatagttaa
      721 acgaagatct ttagtttttg aagaccaccg caagtgttcg tctggcttca catggcatct
      781 tcttcttaga aaaagatcga catattttgt gacacgaatt gcaaatctgg ttttgttgta
      841 tggattgcgt gattttcgat ctggtataac aggtataaag gtgcaccacg atagacaatg
      901 agacagggca tcttgcaatc tatggcaaac atcacttcag ttctttctca tcgggtgatg
      961 aaaacgcact tcagcctgaa aggaatatga aaatgagatc aacagacact ctattttatg
     1021 actctgggta aaatgaattg agtaagtgat atagcttacg aacattcaaa tcaattaaac
     1081 atcagaatag attttatact caggtattta atctggctct ctgtttattt aaataatgtg
     1141 gaaagagatt tttcacagga gacattatac aaaaaatata aaatacagct accggttgcc
     1201 aaagacacta taagcctggc aaaaaaatat tacacaacat aaatgctaat tgtttatgcg
     1261 ggctttgtat tgctttctgt atcctacaaa tgagtgaaat ttatgaaaaa ggctaaaata
     1321 ctttctggcg tattattact gtgcttttcg tcaccattaa tttctcaggc tgcgacactg
     1381 gacgtacgtg gtggatatcg tagtggaagc cacgcctatg agactcgact caaagtcagt
     1441 gagggatggc aaaatggatg gtgggcaagc atggaaagta atacctggaa taccattcat
     1501 gataataaaa aggaaaatgc cgcactcaat gatgttcagg ttgaagttaa ttacgcgatt
     1561 aaacttgatg atcaatggac ggtgcgcccg ggaatgttaa cgcattttag cagcaacggc
     1621 acacgctacg gaccctacgt aaaactgtcc tgggacgcga caaaagatct taattttggc
     1681 attcgctatc gttacgactg gaaagcttac cgacaacaag acttatccgg tgatatgtct
     1741 cgtgataacg ttcatcgttg ggatggatat gtcacttacc atattaatag tgatttcacc
     1801 ttcgcatggc aaacgacgct atacagcaaa cagaacgatt atcgctatgc aaaccataag
     1861 aaatgggcga cggaaaatgc atttgttcta caataccata tgacgcccga tattacgcca
     1921 tacatagaat atgactacct ggaccgtcag ggtgtttaca acggcagaga taatttatcg
     1981 gaaaacagtt atcgcattgg tgtgtcattt aaactgtagt agacaggaga cagtcacaat
     2041 gaataaaaca ataacggcgc ttgctatcct aatggcttca tttgccgcaa acgcgtctgt
     2101 attaccggaa actcctgtac catttaaaag tggtaccgga gcaattgata acgacactgt
     2161 ctacattggt ttaggtagcg caggtacggc atggtacaag ctggagacac aggccaaaga
     2221 taaaaaatgg acagcgttag ctgcattccc tggtggacca agagatcaag caacctcggc
     2281 atttattgat ggcaatctgt atgtgtttgg cggcattggc aaaaacagcg agggcttgac
     2341 tcaggtattt aatgacgtac acaaatacaa ccccaaaacc aatagctggg ttaaattgat
     2401 atcgcacgcg ccgatgggca tggcgggcca tgtgactttt gtacacaacg gcaaggctta
     2461 tgttactggc ggtgttaacc agaatatctt caatggctat tttgaagatc tcaacgaagc
     2521 tggaaaagat tcaaccgctg tagataaaat caatgcacac tattttgaca aaaaagcaga
     2581 agattatttc tttaataagt ttctgttgtc ttttgatccc tctacacagc aatggagtta
     2641 cgctggcgaa tctccctggt acgggacggc tggtgcggcg gttgtgaata aaggtgataa
     2701 aacctggctt attaatggcg aagccaaacc cggattgcga acggatgccg tatttgaact
     2761 tgatttcacc ggtaataatt taaaatggaa taggcttgct cccgtctcat caccagatgg
     2821 cgtcgctggc ggttttgcgg ggataagcaa tgattctctt atatttgccg gaggggccgg
     2881 attcaaaggt tcacgagaaa attaccaaaa cggtaagaac tatgcgcatg aaggcctgaa
     2941 aaaatcatat agcactgata ttcatctttg gcataacggg aaatgggata aatcgggtga
     3001 attatcgcaa ggtcgggcct acggagtatc attgccctgg aataatagtc tattgattat
     3061 tggcggtgaa actgcaggcg gcaaagcggt gacggattca gttttgatct ctgtgaagga
     3121 taataaagtc acagtacaaa actaacgctt cagggccccg gtaaaggggc cctgctatca
     3181 atttgtcatt tcaattacgc gaaatttata tgaacgcaat aatatcgcct gattattact
     3241 atgttcttac tgttgctggt cagtctaatg ccatggcgta tggcgaagga ctgccattac
     3301 cggacaggga agatgcgcct catcccagaa ttaaacaatt agcgagattt gcgcatacgc
     3361 atcccggagg cccgtcatgt cactttaacg acattattcc actgactcac tgcccacacg
     3421 atgttcagga tatgcagagt tatcaccatc ctctggcaac gaatcatcaa acacagtacg
     3481 gcaccgttgg ccaggcactg catattgcac ggaaattact gcccttcatt cctgataatg
     3541 cagggattct catcgttccg tgttgccgtg gcggatcggc ttttaccgca ggcagcgagg
     3601 ggacatattc agaacggcac ggagccagcc atgatgcttg tcgttgggga acggatactc
     3661 cgctatacca ggatttagtc agcagaacgc gagccgcact ggtaaaaaat ccgcagaaca
     3721 aattcctcgg cgtatgctgg atgcaaggcg aatttgactt aatgaccagt gactacgcgt
     3781 cacatcctca acactttaat catatggttg aagcctttcg tagggatcta aaacaatacc
     3841 attctcagct taataatatt actgacgcgc cgtggttttg cggcgatacc acctggtact
     3901 ggaaagaaaa tttccctcat gcgtatgaag ctatttatgg caattatcaa aataatattt
     3961 tagctaatat tattttcgtc gacttccagc aacaaggtgc aagagggctg acgaacgcgc
     4021 ctgatgaaga tccggacgat ttaagcacgg gatattacgg ttcagcgtac cggtcaccgg
     4081 agaactggac gacggcactg cgaagcagtc atttcagctc agcagcccgt cgggggatta
     4141 tttctgacag gtttgtagaa gcaattttgc agttttggcg cgaaaggtga gcgttcatta
     4201 atttgattta ttcgcatcca tcagcgcttt taactcctcc ggcgtgcggc actataacga
     4261 cttccacaac aaggagccat tttcttatac cactttcagt gaccatccgc ttatgccacc
     4321 accctctacg caactgtttg cagagcacct tcccaccgag tggatacagc acttcctgac
     4381 gctttctgct catgcgaccg ttcgtcgtct taacctgagc gtggatggcg aagcggggat
     4441 gaacctgctg gcgcccgcag cgctgtcacc caggcgcgtc agcgcgtggg ggccgccccg
     4501 gtggaatggc tcttccgcca gaccacacag acatggggcg cggaacgtta cctgaaggat
     4561 gactggcacg gcctgcaact ttttgccatt gatggcgcac agttcaggac acctgatgaa
     4621 cccgagctgc gtgaatatta tggctctgcc aacacatcca ctgagcggca gagcgcctac
     4681 cccgtcatgc gtctggtggc gttaatgaat ctgggaatca cattctactg aatgccatga
     4741 ccgcgcctac cggcggagcg atacggcgtg aggcaacaaa agcggcgtca gagatcagct
     4801 ttaagttcgc gttccagttt atcgtgacag aaatgatagt gctgggaaac acggcgtcac
     4861 cggggactat cccgaaacga ctggagcatc tgcgggggct ctggaagtgg tgttcataac
     4921 aaaacgcccc cgaccatcaa gggcgagggc ggttaagata tcaaaaaccc gttatcctgt
     4981 gaaacacagt gccgccccgc ttaagtgaac ggcattgtgt ttctgaggga ccttaatttt
     5041 ccctgattct ctgacgttcc ttaccattga tatttccttc attcctcacc agagaaataa
     5101 aaaatcacta
//
LOCUS       DBFIFI010000262         5086 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.262, whole genome shotgun sequence.
ACCESSION   DBFIFI010000262 DBFIFI010000000
VERSION     DBFIFI010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5086
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.262"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..620)
                     /locus_tag="N7B39_004432"
     CDS             complement(<1..620)
                     /locus_tag="N7B39_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HGU2424283.1"
                     /translation="MQDIHGESLIESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEAVTWQGREYQAYPIDGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSELTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCS"
     gene            complement(620..949)
                     /locus_tag="N7B39_004433"
     CDS             complement(620..949)
                     /locus_tag="N7B39_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU2424284.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSA
                     EFKQVVN"
     gene            complement(946..3591)
                     /locus_tag="N7B39_004434"
     CDS             complement(946..3591)
                     /locus_tag="N7B39_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU2424285.1"
                     /translation="MAGNFADLTAVLTLDSARFSEEAARVKKELGETSALADLMSGKV
                     SQSFRKQADAAEQSLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIVTQLAGGQNPFL
                     IMLQQGGQISDSFGGPLSLLTLLKEELLGIRDASESSEESLSDTANALAENARNAGEL
                     GRFMSVARVAAGGGVAVLAALAAAAWQAEQADRALLRSLTLTGGAAATTTAELWKMAG
                     VISDEAGGGIRQAAENLARLAESGKYTAGQLRIMGETSQRWLQTVGDDAGKVEKAFEG
                     IAADPVKALASLNQQYNFLSVSQLRHIDELERTKGKQAAVTEAMSLFADVMNARLEQL
                     DKAATPVEKIWDDVKTWTSDAWAWIGDHTLGALSLITDVVAGTVEQVKLLLVQGDLAL
                     AEFIQSAWETTKNVPGVGALFGELAEENRVFIEKTKRDELALRKSIAERDARIRQGEM
                     GYINRSRATGVSKGPGQQEAVSRLAEELTGKKHTSPKTRSAGEREEEQAREALLALEA
                     ELRTLEKHSGANEKISRQRRDLWKAESQYAVLKEAATKRQLSEQEKSLLAHKDETLEY
                     KRQLAELGDKVEYQKRLNELAQQAVRFEEQQSAKQAAISAKARGLTDRQAQRESEAQR
                     LRDVYGDNPAALAKATSALKNTWSAEEQLRGSWMAGLKSGWGEWAESATDSFSQVKSA
                     ATQTFDGIAQNMAAMLTGAEADWRGFTRSVLSMMTEILLKQAMVGIVGRIGSAIGGAF
                     GGGASASTGTAIQAAAANFHFATGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGN
                     LYRLMRGYAEGGYVGGAGSPAQMRRTEGINFNQNNHVVIQNDGTNGQAGPQLMKAVYD
                     MARKGAQDELRLQLRDGGMLSGSRR"
     gene            complement(3641..3943)
                     /locus_tag="N7B39_004435"
                     /pseudo
     CDS             complement(3641..3943)
                     /locus_tag="N7B39_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310194.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
     gene            complement(3970..4392)
                     /locus_tag="N7B39_004436"
     CDS             complement(3970..4392)
                     /locus_tag="N7B39_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HGU2424286.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRFDYIKFVSDAEQQETTKHD
                     VVHINQRYLETASLLVAMSLWHSHSLKGTLASPETEMQQIRREVMLGWPADALNQATN
                     RVLYLSGMLDNRHDADPEPTGKTEATEPVTSKKHSKAS"
     gene            complement(4406..>5086)
                     /locus_tag="N7B39_004437"
     CDS             complement(4406..>5086)
                     /locus_tag="N7B39_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU2424287.1"
                     /translation="DAYANPLSDDDWQRLAKVKDLTPGEMTAEPYDDNYLDDEDADWT
                     ATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFESGDVRAYKIRFPNGTVDVFRGWVSS
                     IGKAVTAKEVITRTVKVTNVGKPSVAEERSEITPATAIKVTPTSGTVAKGKTTTLTVS
                     FEPESATDKTFRAVSADPSKATISVKDMTITVNGVATGKVQIPVVSGNGQFAAVAEVT
                     VTEAGAAG"
BASE COUNT          997 a         1717 c         1176 g         1196 t
ORIGIN      
        1 ttgctgcatc tgtccttacg gatatccgtg gtggggttgt cgaactcatc cgccaccgca
       61 ggaccgttat acccgcattc atctccccgg taatcccaca tacaggtatt cgccagcata
      121 atgcgaccgg gaaacagcgc tccgtccgtc tccgtcggtg ttgccagcac aaacgaggct
      181 gtcatggccg tcagctctga catctgctcc accacccagc ggtcgctcag ctcctgctcc
      241 gggtccgctt ccggattgcc cgccacaaaa ttcaccgcat ccagaaaacg ggcatacacc
      301 cggcggcgga ccaccgtggc ccccaccagg ctctgcaggt cctccgccat tccggtgacc
      361 agaccgaaca gattcgacac cgtcagcgac gggcgggcac tgctgccctt cccgttcatc
      421 tcaaaaccgc tgccgtcaat cgggtatgcc tgatattccc gcccctgcca ggtaaccgcc
      481 tccccttttt cattcagctc attgcagaaa aaataccgct caccaccctg caccgtcagg
      541 tcgatttccc agagcaccac ccgcggtgac tgctccgatt taaccgactc gatcaggctt
      601 tccccgtgaa tatcctgcat cagttcacca cctgcttaaa ctccgcgctg aactcaacgc
      661 gcaacatccc gacccgcgca gaccacccgg cacaggtcac ctttatctgc cggtatgcat
      721 agggtggctt ccacaaaaat gccttccagc ctccgtgctc tgccaggaat gcctccagat
      781 gtcgggcctc ctcccgggtc acggaaagcg tcacacggta tgttttcagg tcagcattca
      841 gccccgccgc catacgctgc gaatacccgt caccaaaacg cacttcacgc accgatggct
      901 gcgagttcac ctccatatcc ggcttcactt tccagcgaaa tgttttcatc gcctgctccc
      961 cgataacata ccgccatcac gcaactgcag ccggagctca tcctgcgccc ccttgcgggc
     1021 catgtcatac accgccttca tcagctgcgg ccccgcctgt ccgttggtgc cgtcgttctg
     1081 aatcaccacg tgattgttct gattaaaatt aatgccttcc gtccgccgca tctgcgccgg
     1141 acttccggca ccgcccacat aacccccttc cgcatagccc cgcatcaggc ggtacaggtt
     1201 gccgacgcca atccggctgg ttgcctcctt cgtgaagaca aactccccgc gatgaacaat
     1261 tcccgcaggt tcatatttac cccccgtccc cgtaaatccc ccggtcgcga aatggaagtt
     1321 cgccgccgca gcctgaatgg ccgtccccgt ggaggcagac gcaccaccac cgaaagcacc
     1381 gccaatggcg ctgccgatac gcccgacaat gcccaccatg gcctgtttaa gcaggatttc
     1441 tgtcatcatg gacagcaccg aacgggtgaa tccccgccag tctgcctctg caccggtcag
     1501 catcgccgcc atattctgtg caataccgtc aaaggtctgc gtggcagcac ttttaacctg
     1561 cgaaaaactg tccgtcgcac tttccgccca ctcgccccag ccggacttca gcccggccat
     1621 ccagcttcca cgaagctgct cctccgcaga ccaggtgttc ttcagtgccg atgtggcctt
     1681 cgccagcgca gccggattat caccgtacac gtcccgaaga cgctgcgctt cagactcccg
     1741 ctgcgcctga cggtcagtga gaccgcgggc ttttgcgctg atggcggcct gcttcgcgct
     1801 ctgctgctct tcaaaccgca ccgcctgctg tgccagctca ttcaggcgtt tctggtattc
     1861 aaccttgtcg cccagctcag ccagctggcg tttgtactcc agcgtctcgt ctttatgcgc
     1921 cagcagggat ttttcctgct cagataactg tcgtttcgtg gcagcctctt tcaggaccgc
     1981 atactgactt tccgccttcc ataaatcacg gcgctgccgg ctgattttct cattcgcacc
     2041 gctgtgtttt tccagcgtcc tgagctcagc ttcaagggca agcagagcct ctcttgcctg
     2101 ctcctcttcc ctctccccgg cagagcgcgt tttcggtgat gtatgctttt tacctgtcag
     2161 ctcttcagcc agacggctga cggcttcctg ctgccccgga cctttgctga cgcctgttgc
     2221 acgcgaacgg ttgatgtacc ccatttcccc ctggcgtata cgcgcatccc gttccgcaat
     2281 ggattttctc agcgccagtt catcacgttt tgttttctca ataaatacgc ggttctcttc
     2341 tgccagttca ccaaacaacg caccaacgcc gggcacattc tttgtcgttt cccaggctga
     2401 ctgaataaat tcagccagcg ccagatcccc ctgcacaagc agcagcttca cctgttcaac
     2461 ggttcctgcc accacgtcag tgatcagact gagtgccccc agtgtatgat cacctatcca
     2521 tgcccatgcg tcagaagtcc aggttttaac atcgtcccag attttttcca ccggcgtggc
     2581 cgctttatca agttgctcca gacgtgcatt catgacatcc gcaaacaggg acatcgcctc
     2641 cgtcaccgca gcctgtttac ctttcgtgcg ctcaagctca tcaatatggc gtaactggga
     2701 aacgctcagg aagttatact gctgattcag ggaggccagc gccttcaccg gatctgctgc
     2761 aatcccttca aaggcttttt ccaccttccc ggcatcgtcc cccaccgtct gcagccatct
     2821 ctgagaggtt tcccccatga tccgtagctg cccggcggta tatttcccgc tttctgccag
     2881 acgggccaga ttttctgccg cctgtctgat accaccaccg gcttcatcgc tgatcacccc
     2941 ggccattttc cacaattctg ccgttgtggt ggcagcagcc cctccggtca gggtcagtga
     3001 acgcaataag gcccggtcag cctgctctgc ctgccaggcg gcggcagcaa gcgcggccag
     3061 tacggcaacc ccgccacctg ccgccacacg ggccaccgac ataaatcgtc ccagctcacc
     3121 ggcattccgg gcattttcag ccagtgcatt tgccgtatct gacagcgact cctctgatga
     3181 ttcagaggca tccctgatcc cgagaagttc ctccttcagc agggtaagca ggctgagcgg
     3241 tccaccgaat gaatcgctga tctgcccccc ctgctgcagc atgataagga agggattctg
     3301 accaccggca agctgagtga caatatccgt gaactgtgcg ggcagtgtgc gcatggcagc
     3361 cttatactgt ccgactgata tcccggcttt ttgtgcagcc agcgcctgtc ggctcagact
     3421 ctgctcagca gcatcagcct gttttctgaa agactgactg actttcccgg acatcaaatc
     3481 agcaagcgca ctggtttcac ccagctcttt ttttacccgc gctgcctctt cagaaaaacg
     3541 ggcagaatcc agtgtcagca cggctgtcag atcggcaaaa ttacctgcca tcgtggtcac
     3601 ctcctgaaat gtcctctgat accatcaata actgtcacaa cctccttccc tcctccccga
     3661 aacggactcc accggcgagg cccgccgcct tctgcatcag tacatcattt tcgtccggcg
     3721 tctccgcctg tccttcctct gcctccggag cgaacaggct gaaatccgcc ggatgcatat
     3781 ccggatcgca aaaaaacagg ctgagtacgg cgtacatcag cccggaaaaa tgcatatcca
     3841 gctgggtatc ctgaaaataa tgcgtgcagt aaaaacgtcg ccagtcggca tattcggtgg
     3901 atgtcatccc ggcaagcatg gcgcgccagt cggctctctc catctcacgc gccagtttca
     3961 ggacaaagtt cagctcgcct tcgaatgctt ttttgatgtt accggctcag tcgcttctgt
     4021 tttcccggtt ggttcaggat cggcatcgtg ccggttatcc agcatacctg aaagataaag
     4081 cacccggttc gttgcctgat tcagtgcatc agcaggccat cccagcatca cttcacggcg
     4141 gatctgctgc atctctgtct ccggagaggc cagagtgcct ttgagggaat gggaatgcca
     4201 tagcgacatc gccacaagca gggatgccgt ttccagatat cgctggttaa tgtgcacgac
     4261 atcatgcttc gttgtctcct gttgttctgc gtctgaaaca aactttatat aatcaaaccg
     4321 ctgcagcgca gacaattcgg aaagcgtgac ggacacaccg ttatattcaa attgttctgt
     4381 tttcagaaac atgtattacc tccgtttacc ctgcagcgcc cgcttcagta acggtgactt
     4441 cagccactgc ggcgaactga ccatttccgc tcaccacagg gatctgcacc ttacctgtcg
     4501 ccacgccgtt taccgtaatt gtcatatctt tcacactaat ggtggctttc gacggatcgg
     4561 cggaaaccgc tctgaacgtc ttgtcggttg cactttccgg ctcaaaagaa accgtcaggg
     4621 tggttgtttt cccttttgcc accgtaccgg atgtcggtgt caccttaatt gcagtggccg
     4681 gcgtaatttc gctgcgttct tccgccacgg aaggtttgcc cacgttagtg actttcaccg
     4741 tgcgggtgat cacttctttc gccgtcacgg ccttaccgat actgctgacc cagccacgga
     4801 acacatccac cgtgccattt gggaaacgga ttttataggc ccgcacatcc ccgctttcaa
     4861 accagcctat aagccctttc tgaccttctt ctcccggttt ccaggccagc gtaaaactgg
     4921 tatctcctgc agacttctgc ccctgcccgg tcgcggtcca gtccgcgtct tcatcatcca
     4981 ggtagttatc atcgtagggt tctgccgtca tctcgcccgg cgtcagatcc ttcaccttag
     5041 ccagtcgctg ccagtcatcg tctgacaacg ggtttgcata agcatc
//
LOCUS       DBFIFI010000263         5007 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.263, whole genome shotgun sequence.
ACCESSION   DBFIFI010000263 DBFIFI010000000
VERSION     DBFIFI010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5007
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.263"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(172..1836)
                     /gene="glnS"
                     /locus_tag="N7B39_004438"
     CDS             complement(172..1836)
                     /gene="glnS"
                     /locus_tag="N7B39_004438"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HGU2424288.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRTGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRSD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(2039..3985)
                     /gene="nagE"
                     /locus_tag="N7B39_004439"
     CDS             complement(2039..3985)
                     /gene="nagE"
                     /locus_tag="N7B39_004439"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HGU2424289.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            4318..>5007
                     /gene="nagB"
                     /locus_tag="N7B39_004440"
     CDS             4318..>5007
                     /gene="nagB"
                     /locus_tag="N7B39_004440"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HGU2424290.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQ"
BASE COUNT         1242 a         1346 c         1241 g         1178 t
ORIGIN      
        1 tgcggcgtga acgccttatc cggcctacaa aagcacgcaa actcaatata ttgcagagat
       61 catgtaggcc tgataagcgt agcgcatcag gcaatttagc gtttgaaact gtttgtctgt
      121 tccttgccgg atgcgacgta aacgccccat ccggcatagc gaaacttaaa attactcgcc
      181 tactttcgcc caggtatcac gcagcccaac ggtgcggtta aataccggtt tttccgccgt
      241 agaatggcgg ctatcgaggc agaagtaacc ttcacgctca aactggaatg ctttacccgc
      301 aaccgcatct ttcagcgacg gttcagcaaa gccctgtttg atcaccagcg attccgggtt
      361 aatcaccgac aggaaatcat ccgcagcacc tgggttcggc acgctgaaca gacggtcata
      421 caaacggatt tcaaccggca gcgcatgtgc cgcgctcacc cagtgaataa cacctttgac
      481 tttacgacca tctgccggat ctttgcttaa ggtatcggcg tcataagtac agaagatggt
      541 ggtgatatta ccttcggcat ctttctcgac gcgttcagcc ttaatcacat aagcattacg
      601 cagacgcact tctttaccca gcaccagacg tttgtactgc ttgttagctt cttcgcggaa
      661 atcggagcga tcaatccaaa tctcaccgct aaacggcacc tgacggctcc ccatttccgg
      721 tttgttcgga tggttcggca tggtaaccat ttcgccttcg ccctgatagt tttcgataac
      781 cagtttcacc ggatcgataa ccgccattgc gcgcggcgca ttttcgttga gatcttcacg
      841 gatgcaggat tccagcgacg ccatctcaat ggtgttgtcc tgcttggtca cgccgatgcg
      901 tttgcagaac tcacgaatag aagccgcagt gtaaccacga cgacgcagac cggaaatggt
      961 cggcatacgc gggtcatccc agccttcaac gtgcttgtcg gtcaccagca agttcagctt
     1021 acgcttggac atcacagtgt attccagatt caggcgcgag aattcgtact ggcgcgggtg
     1081 aacaggaatg gtgatgttgt ccagtaccca gtcgtacaga cgacggttat cctggaactc
     1141 aagcgtacac agagagtgcg taataccttc cagggcatcg ctgatgcagt gggtgaagtc
     1201 gtacatcggg tagatgcacc acttgttgcc agtctggtgg tgttcagcaa atttaatgcg
     1261 gtacagcacc ggatcgcgca tcacgataaa cggtgaagcc atgtcgattt tcgcacgcag
     1321 gcaggcttta ccttcttcaa aaccgccggt acgcattttc tcgaacagcg ccaggttctc
     1381 ttcaacgcta cggtcgcggt acgggctgtt tttaccaggc tgcgtcaggg tgccgcggta
     1441 ttcgcggatc tgttccggcg tcagttcatc aacgtacgcc aggcctttat tgatcagttc
     1501 gatcgcatag gcgtggagct gatcaaaata atcggaggag taacggacgt taccagacca
     1561 gtgaaaacct aaccactcaa cgtcgttttt gatcgaatcg acatactcga tatcttcttt
     1621 taccgggtta gtgtcgtcga aacgcaggtt gcactggcct ttatagtcct gggcgatccc
     1681 gaagttcagg cagatagatt tcgcatggcc aatatgcaga tagccattcg gctccggcgg
     1741 gaaacgggtg tgtactgtgg tgtgcttacc actggccaga tcttcatcga tgatctgacg
     1801 gataaagtta gtcgggcggg cttctgcctc actcatcgtg gattcctcaa agcgtaaaca
     1861 acgtataacg gcgtatgatc ttataagcgg gacaggctga caactgttag ttagtgaaaa
     1921 tcgaaaatct cactgagatt atggggatga ttgatgaaaa aaaaagcggc agagaagatc
     1981 tccaccgctt gtcattgttg gatgcgacgc tcaagcgtcg catcaggcat aaagcagatt
     2041 actttttgat ttcatacagc ggtgtttgac ccgccacaac atggccctga gctttaatga
     2101 tcaagccact gaaatcgtcg atattgctgc aaaccaccgg gctaatcatc gaacgggcgt
     2161 tagcgttcag gtaatccaga tccatttcca gaatcggttg ccctgcgctt acctgcgcgc
     2221 cctcttccac cagacgttta aagcctttac cttccagcgc tacggtatcg atacccatgt
     2281 gaacgacaat ctccgcgcct ttttcggttt ccaggcagaa cgcgtggttg gtgttgaaga
     2341 ttttcacgat tgttcctgcg gctggtgata cgacgatttt atctgtcggt ttcaccgcca
     2401 caccgtcacc caccgctttg ctggcgaatg cttcgtcagg aacctgatcc agtgccacga
     2461 catcaccggt aatcggcgat accagctccg cgatagatac cgcgtttggt acagcctgcg
     2521 gttttgctac aggagcggca gttgccggag ttgcttcagc agaggcagct gctaccgggc
     2581 cacgggcaac gactttcttc atcgcatcgc cgatggattc tgctttcgcg ccaacaatca
     2641 cctgaatagt ctgtttgttc agttttacta ccccagaagc ccccagacgt ttacacatcg
     2701 tatcgttaac gcgggcagag tcagccacgg taaggcgcag acgggtgata caggcgtcaa
     2761 ttgctttcag gttgtcagtg ccgccaaccg cagcaatata gttggtcgcc agttgattga
     2821 ggccttcttc agtgttgctg ttggcttctt cagtaacgat ctcgtcttct ttatcttcac
     2881 gacccggcgt tttcaggttg aacatgcgga taaccaaact gaacaccacg aagtagatag
     2941 cgaagaagac aacacccata accagcagca tccagacgtt ctggctggcg gccggcaggt
     3001 tatacatcaa cgcgtagtcg atagcccccg cagagaaaga gaagccggca tggataccca
     3061 gcagcgttgc cacaaacagg ctgataccgg tcagcagtgc gtgcaggagg tacagcagcg
     3121 gagcaaggaa catgaacagg aattccagcg gctcagtcac accggtcagg aacgcagtaa
     3181 cagcaacaga aagcagcatc ccgccaacca tcggacgacg ctctttcggt gctgcgaagt
     3241 acatcgccag cgccgcaccc ggcaggccga acatcatgat cgggaagaag ccggacatga
     3301 acatccccgc ggtgccgtca ccagcgtaga aacggttgat gtcgccgtgg aaaaccgtac
     3361 ccgccgcgtt ggtgaattca ccaatctgga accaggcgat ggtgttcagc acctgatgca
     3421 gaccggttgg gatcaacaga cggttgatga aaccaaagat accggaaccc agcgcgcccg
     3481 cagaaacgat ccactcgccg cctgcatgga tagcgtgctg taccggcggc cagacgtaac
     3541 caaaaatggc cgccagcacc agacagaaga agccggtggc gatcggcaca aagcgtttgc
     3601 cgccgaagaa gctcaggaag tccggcagtt taatatcgga ccaacggtta taggctgcgc
     3661 caccaaccag accggtaatg atacccgcca gtacacccat gttaatttct gggttgatgg
     3721 tcaccatcgc tttggttaac acaaagtaac ctaccgcccc cgccagcgcc gccgcacctg
     3781 cgctgtcttt cgaccagctg gatgccacac cgatggcgaa gattaatgcg aggttatcaa
     3841 aaatcgcacc gcccgcctgg gcaataaacg caacgttaag taaatctggc tgaccgaatc
     3901 gcagcaacag tgccgccacc ggcagcaccg cgatagggag ctgtaacgcc ctaccgagtc
     3961 gctggaaaaa acctaaaata ttcatcttat tccccctacg agaaccctat ttggctcgtt
     4021 taaagccgta tttttattgt gctgcaaatt gtactgccga tgttctgtaa tcagattgtt
     4081 agatcatctg ccacagagtg tgtgaaaatt taattcgtat cgcaaattaa acgagtgtct
     4141 tttgtgagtt ttgtcaccaa atatcgttat catcactccc ttttactggc taaaccagaa
     4201 aacttatttt atcattcaaa aaatcaggtc ggattgacgc ctgtctgcgc aaatccaggt
     4261 tacgcttaaa gatgcctaat ccgccaacgg cttacatttt acttattgag gtgaataatg
     4321 agactgatcc ccctgactac cgctgaacag gtcggcaaat gggctgctcg ccatatcgtc
     4381 aatcgtatca atgcgttcaa accgactgct gatcgtccgt ttgttctggg cctgccaact
     4441 ggcggcacgc caatgaccac ctataaagcg ttagtcgaaa tgcataaagc aggccaggtc
     4501 agctttaagc acgttgtcac cttcaacatg gacgaatatg tcggtctgcc gaaagagcat
     4561 ccggaaagct actacagctt tatgcaccgt aatttcttcg atcacgttga tattccagca
     4621 gaaaacatca accttctcaa cggcaacgcc ccggatatcg acgccgagtg ccgccagtat
     4681 gaagaaaaaa tccgttctta cggaaaaatt catctgttta tgggcggtgt aggtaacgac
     4741 ggtcatattg catttaacga accggcgtct tctctggctt ctcgtactcg tatcaaaacc
     4801 ctgactcatg acactcgcgt cgcgaactct cgtttctttg ataacgatgt caatcaggtg
     4861 ccaaaatatg ccctgactgt cggtgttggt acactgctgg atgccgaaga agtgatgatt
     4921 ctggtgctgg gtagccagaa agcactggcg ctgcaggccg cagttgaagg ttgcgtgaac
     4981 catatgtgga ccatcagctg tctgcaa
//
LOCUS       DBFIFI010000264         4997 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.264, whole genome shotgun sequence.
ACCESSION   DBFIFI010000264 DBFIFI010000000
VERSION     DBFIFI010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4997
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.264"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(325..900)
                     /locus_tag="N7B39_004441"
     CDS             complement(325..900)
                     /locus_tag="N7B39_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HGU2424291.1"
                     /translation="MAVSLVKGGNVSLTKEAPTMNVAMVGLGWDARVTDGQGFDLDAS
                     VFAVGEDGKVLSDAHFIFFNNKTSPDGAVEHQGDNRTGEGDGDDEQVKIDLTKVSADI
                     KKLVFAVTIYDAEARKQNFGMVSNSFMRVYNNDNGTEIARFDLSEDASTETAMVFGEL
                     YRHGTEWKFKAVGQGFAGGLSALASQHGVNV"
     gene            complement(969..1547)
                     /gene="terD"
                     /locus_tag="N7B39_004442"
     CDS             complement(969..1547)
                     /gene="terD"
                     /locus_tag="N7B39_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerD"
                     /protein_id="HGU2424292.1"
                     /translation="MSVSLSKGGNVSLSKAAPSMKNVLVGLGWDARSTDGQDFDLDAS
                     AFLLASNGKVRGDSDFIFYNNLTSSDGSVTHTGDNRTGEGDGDDESLKIKLDAVPSEV
                     DKIIFVVTIHDAQARRQSFGQVSGAFIRLVNDDNQTEVARYDLTEDASTETAMLFGEL
                     YRHNGEWKFRAVGQGYAGGLASVCAQYGINAS"
     gene            complement(1596..2636)
                     /gene="terC"
                     /locus_tag="N7B39_004443"
     CDS             complement(1596..2636)
                     /gene="terC"
                     /locus_tag="N7B39_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerC"
                     /protein_id="HGU2424293.1"
                     /translation="MVSTHIGFPTETVIVFIALSVGAIFIDLFMHRDDKPISLKSAAL
                     WSVFWVVVAMAFAGFLYIHHGAEVASLFVTGYALEKVLSVDNLFVMMAIFSWFAVPDR
                     YRHRVLYWGIIGAIVFRGIFVAIGTSLLSLGPYVEVVFAIIVAWTAVMMLKSGDDDDE
                     IEDYSQHLAYRMVKRFFPIWPKLRGHAFLLNQKEVDAELAKPENSDVTIGRGKKAALY
                     ATPLFLCVAVVELSDVMFAFDSVPAIIAVSREPLIVYSAMMFAILGLRTLYFVLEALK
                     QYLVHLEKAVIVLLFFIAAKLGLNATDHIWHHGYSIAATTSLYVVLGVLALGILASVM
                     FPGKPESEEKGS"
     gene            complement(2659..3114)
                     /locus_tag="N7B39_004444"
     CDS             complement(2659..3114)
                     /locus_tag="N7B39_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HGU2424294.1"
                     /translation="MSFFDKVKGAINSGRDELTRQVGRFKNKKFMQGTVAVCARIAVS
                     SDGVSSEEKQKMMGFLRSSEELKVFDTNEVIEFFNKLVSSFDFDVEIGKGETMKYILA
                     LKDQPEAAQLALRVGIAVAKSDGNFDQDEKLASREIAIALGFDPAEFGL"
     gene            complement(3137..4294)
                     /locus_tag="N7B39_004445"
     CDS             complement(3137..4294)
                     /locus_tag="N7B39_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HGU2424295.1"
                     /translation="MNLTPGGNAPVPAQELRVRITSGGQVDASAFRLYADGKVQGDAD
                     MVFYGQPRNDDGTVSLVSEGQYSTFTVALNRLKPDVQKIAFTVTCDGGQTVSGLRNLS
                     IDVEQGATGLVSGSVELSGRQEAALILGEFYRRNNDWKFRFVAQGFNGGLKPLAEHFG
                     VNIADEPAPAAPTPPVVTPPPVETKISLSKVSLTKEKPAISLTKRDNFGEIRINLNWH
                     RGSSKSGFAGMFGSKGIDLDLGAFVELQDGYKSVIQALGNAFGDYRDEPYVQLKGDDR
                     TGDVSDGEWLHINGREWKHIREVLIYAFIYEGVPSWDKTDGVVTIHVPDQPPIETRLT
                     EGENRRTLCAIARLVNENGAIKVERINQYFKGQDEMDRAFGWGFRWSAGSK"
     gene            complement(4294..4875)
                     /locus_tag="N7B39_004446"
     CDS             complement(4294..4875)
                     /locus_tag="N7B39_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HGU2424296.1"
                     /translation="MVSLVKNQTVSLSKESSALSQLHFGLGWDPVKKKGLLGGLFGGN
                     DSIDLDAGCVLMDSTGKTIDTIWFRKLKSTCGAVVHSGDNLTGEGDGDDEVIKVNLSR
                     LPANVEYLAFTVNSFRGQSFNDVENAFCRVVDQTGKELARYKLTEQGSHTGIVISSLR
                     RNNGNWDFTALGHACRGRTIDDMHSDIVSAVIR"
BASE COUNT         1262 a         1345 c         1197 g         1193 t
ORIGIN      
        1 tggtatgaca ccatttttca taacgctgaa gtttctatac ctgttgaatt tgaattttca
       61 ttgaccgggt atcttatttt ccagggcccg ttccttcata agtcgcaaaa gtaacatata
      121 tccgaagggc atgctgttga tatcagacac ggaatactgg ctttaaccag caaccacaga
      181 taaacccggg gcctgcataa aaggttcatg ccagaattaa catagctctg ctttctgccc
      241 cccccctttc ccatattcac ccgttgatag cggatcgata cccaaaaaaa accccggcat
      301 tgccggggtc agactcagcg tcttttagac attgacgccg tgctgggaag caagcgctga
      361 cagaccaccg gcaaaaccct ggccaacagc tttaaacttc cactcagtac catggcgata
      421 cagttcaccg aagaccattg cggtttcggt tgaggcgtct tcagacagat cgaaacgggc
      481 aatttccgtc ccgttgtcgt tgttgtaaac gcgcatgaag ctgttgctca ccatgccgaa
      541 gttttgttta cgcgcttctg catcatagat ggtaacggca aataccagtt ttttgatgtc
      601 tgctgagact ttggtcagat cgattttgac ctgctcatcg tcgccgtcgc cttcaccggt
      661 acggttgtcg ccctggtgct ctactgcgcc atcagggctg gttttattat tgaagaaaat
      721 gaaatgggca tctgacagta ctttaccgtc ttcacctact gcgaatacgg aagcgtccag
      781 atcaaaaccc tgaccatcgg ttacacgggc atcccagccc aggccaacca tagcgacatt
      841 catggttggt gcttctttgg tcagagatac gttgccgcct tttacgagag aaactgccat
      901 ttttagctcc tgcaaacagt tgaatgaggc tgaattaaca gccccagata tgaaaagtta
      961 cttttcgatc aggacgcgtt aatgccgtac tgagcacata cagatgccag accaccagca
     1021 taaccctggc ctactgcgcg gaatttccac tcaccattgt ggcgatacag ctcgccgaac
     1081 agcatggcag tctcagtgga cgcatcttcg gtcagatcgt agcgagcgac ttcagtctgg
     1141 ttatcgtcat taaccagacg aataaacgca ccggatacct gaccaaagct ctggcgacga
     1201 gcctgagcat cgtggatggt cacaacgaag atgatcttgt caacttcaga cgggacggcg
     1261 tccagtttaa ttttcagcga ttcatcatca ccatcgccct caccggtgcg gttatcgccg
     1321 gtgtgcgtta cggaaccgtc ggatgacgtc aggttgttat agaagatgaa atctgaatcg
     1381 ccgcgcactt tgccgtttga ggccagcagg aatgctgaag catccaggtc aaagtcctga
     1441 ccgtctgttg aacgcgcatc ccagccaagg cccaccagga catttttcat tgacggagct
     1501 gctttactca gggagacgtt cccgcctttg gaaagagaaa cactcataaa ataacctctt
     1561 cgattagtaa ttgttcaggt taacacttaa ggggattaac tccccttttc ctcagattca
     1621 ggtttgcccg ggaacatgac gcttgcgaga atgcccagcg ccagtacacc cagtacaaca
     1681 tacaggctgg ttgttgccgc gatgctgtaa ccatgatgcc agatgtgatc ggtcgcattc
     1741 aggccgagtt ttgccgcgat gaagaacagc agcacgataa cggccttctc cagatgaacc
     1801 aggtactgtt tcagtgcctc aaggacaaaa tacagagtac gcagacccag gatagcaaac
     1861 atcatggcac tatagacgat aagcggttca cgactgacgg caatgattgc cggtaccgag
     1921 tcaaacgcga acattacgtc cgagagttca accacagcca cacacaggaa cagcggggtc
     1981 gcatacagcg ccgctttttt accacggcca atggtgacat cgctgttttc tggtttcgcc
     2041 agttcagcat ccacttcctt ctggttaagt aggaaggcat gccctctgag cttcggccag
     2101 atagggaaga agcgtttaac catacggtaa gccagatgct gggagtaatc ctcaatttca
     2161 tcatcgtcat caccgctttt aagcatcatg accgccgtcc aggcaacgat aatagcgaag
     2221 acaacttcaa cgtacggccc cagactcagc aggctcgtac cgatggcgac aaagatgccc
     2281 ctgaagacga tggcaccaat gatcccccag tagagaacac ggtggcgata acgatccgga
     2341 acggcgaacc aggagaagat tgccatcatg acgaacaggt tatcgaccga cagcactttc
     2401 tccagcgcat aacccgtgac aaacagactg gcaacctcag caccgtggtg gatatagagg
     2461 aaaccggcaa atgccatcgc aaccacaacc cagaatacgg accagagcgc agcactcttc
     2521 agcgagattg gcttgtcatc acggtgcata aacaggtcaa taaagatggc accgactgaa
     2581 agcgcaataa aaacaatgac cgtttcagtc gggaagccga tgtgtgtgga aaccatattt
     2641 tacccttagg gatgtggatc agaggccaaa ttcagccggg tcgaagccca acgcgatagc
     2701 gatctcgcgg gaggccagtt tctcgtcctg atcgaagtta ccgtcacttt tcgcaacggc
     2761 aataccaaca cgtaaggcca gctgagcggc ctcaggctga tctttcagcg ccaggatgta
     2821 tttcatggtt tcgcccttgc caatttcaac atcgaaatcg aagcttgaaa ccagtttatt
     2881 gaagaactca atcacctcat tggtatcgaa gaccttcagc tcttctgaag agcgcagaaa
     2941 gcccatcatt ttctgctttt cttccgaact tacgccgtca ctcgatacgg caatacgggc
     3001 acatacagca acggtgccct gcatgaattt tttgtttttg aaacggccaa cctggcgggt
     3061 cagttcgtca cggcctgagt taatggcacc tttaactttg tcgaaaaagc tcatacctgt
     3121 ttcctttgtt gctgtgttat ttagaaccgg cgctccagcg aaatccccag ccaaatgccc
     3181 ggtccatttc gtcctggcct ttgaagtact ggttaattcg ctcgacttta attgcgccgt
     3241 tttcgtttac gagtctggca atggcgcaca atgtgcgacg gttctcacct tcggtcaggc
     3301 gggtctcaat gggcggttga tccggcacat gaatggtgac tacaccatca gtcttgtccc
     3361 agctgggaac accttcataa ataaaggcgt aaatcagcac ttcgcggata tgcttccatt
     3421 cacgtccatt gatatgcagc cactcgccgt ctgatacatc acccgtccgg tcatcgccct
     3481 tgagctgaac atacggttca tcgcgataat caccgaatgc attaccgagc gcctgaatga
     3541 ccgatttata cccgtcctga agctcaacaa aggcccccag atccaggtcg attcctttgg
     3601 agccgaacat acctgcaaag cctgatttgc tactgccccg gtgccagttc aggttgatcc
     3661 ggatttcacc gaagttatcc cgtttggtca ggctgattgc aggcttctct tttgtcagcg
     3721 ataccttgct caggctgatt tttgtctcaa ctggcggagg ggttacaacc ggaggagttg
     3781 gggctgcggg agccggttcg tcggcaatat ttacgccgaa atgctctgcc agcggtttaa
     3841 gtccaccgtt gaacccctgc gcaacgaagc ggaatttcca gtcattatta cgacggtaaa
     3901 attcccccag aattaaggct gcttcctgac ggccgctcag ttcgacactg ccgctaacca
     3961 ggccggtagc cccttgctcc acgtcgatgg aaagattacg aagaccggac accgtctgcc
     4021 caccgtcaca ggtcacggtg aacgcaattt tctggacatc aggtttcagc cggttgagcg
     4081 cgacagtaaa ggttgagtac tggccttcac tgaccaggct gacggtacca tcatcgttac
     4141 gtggctgacc ataaaacacc atgtccgcat caccctgcac cttcccgtcg gcatacaggc
     4201 gaaacgccga agcatccacc tggccgcccg aggtgatccg gacccgcaac tcctgggctg
     4261 gaacgggggc attgcccccc ggcgtcaggt tcattagcga ataaccgctg aaacgatatc
     4321 ggagtgcata tcgtcaattg tgcggccgcg acaggcatgg ccaagggcgg taaagtccca
     4381 gttgccgtta ttacggcgca gcgaagaaat aacgatcccg gtatgtgagc cctgctcagt
     4441 cagcttgtag cgagccagct ctttgccggt ttgatcgaca acgcggcaga aagcgttttc
     4501 gacgtcattg aacgactgac cacggaagct gtttaccgta aacgccaggt attcaacgtt
     4561 tgcaggcagg cgggagaggt tgaccttaat cacttcatcg tcaccgtcac cctcaccggt
     4621 caggttatcg ccactgtgca caacagcgcc gcaggtggat ttcagtttgc ggaaccagat
     4681 ggtgtcaatt gttttacctg tgctgtccat taatacacag ccagcatcga gatcgattga
     4741 gtcgttgcct ccaaacaatc ctccaagaag accttttttc ttaaccggat cccaaccgag
     4801 accgaaatga agctggctta atgcagatga ttctttgctg agtgataccg tctggttctt
     4861 aactaatgaa accatgtttt attccttaaa atggtaatga ccgagacaat caaaggtata
     4921 tttagtaact gacgaagaaa atcatatcat gatgaagttc tctgacaagg gaaatttttg
     4981 tggttttctg taaaaac
//
LOCUS       DBFIFI010000265         4949 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.265, whole genome shotgun sequence.
ACCESSION   DBFIFI010000265 DBFIFI010000000
VERSION     DBFIFI010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4949
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.265"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            486..1268
                     /locus_tag="N7B39_004447"
                     /pseudo
     CDS             486..1268
                     /locus_tag="N7B39_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008815333.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            1388..1575
                     /locus_tag="N7B39_004448"
                     /pseudo
     CDS             1388..1575
                     /locus_tag="N7B39_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611456.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1773..2624)
                     /locus_tag="N7B39_004449"
     CDS             complement(1773..2624)
                     /locus_tag="N7B39_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorothioated DNA-binding restriction
                     endonuclease"
                     /protein_id="HGU2424297.1"
                     /translation="MASSKSLQQAIANIKIWHKGEQRAPHKPLLLLYVLAGYLNGHPR
                     LFDYGTEIYEPLHSLLERFGPQRSQYRPDMPFWRLQGDGIWQLHNAERCSTAGSSRQP
                     PVKELNEYHVAGGFDEQHYALVTGNKKLINTLAQQILEAHFTESIQEEIADELGFDLQ
                     QTRKQRDPLFRKNVLRAYNYQCAICGFNMRHDDTTVALVAAHIKWKQHGGPCEIPNGL
                     ALCAIHHKAFDKGSIGLDENMRVLVSDAVNGGGIVERLFWDFDGKTIALPQVRKNYPY
                     EGFVGER"
     gene            complement(2698..2940)
                     /locus_tag="N7B39_004450"
                     /pseudo
     CDS             complement(2698..2940)
                     /locus_tag="N7B39_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid mutarotase"
     gene            complement(3165..4268)
                     /gene="zorE"
                     /locus_tag="N7B39_004451"
     CDS             complement(3165..4268)
                     /gene="zorE"
                     /locus_tag="N7B39_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II Zorya anti-phage system protein ZorE"
                     /protein_id="HGU2424298.1"
                     /translation="MKLSIDISELIQLGKKMLPEGVDFFLDESPIDFDPIDIELSTGK
                     EVSIEDLDPGSGLISYHGRQVLLYIRDHSGRYDAAIVDGEKGKRFHIAWCRTLDEMRH
                     KNRFERYHATNRIDGLFEIDDGSGRSQDVDLRVCMNCLERLNYKGSIDKQRKREIFKS
                     FSLNEFFSDYSTCFRHMPKGIYDKTNSGYVENWKEISKEIREKANYVCNDCGVNLSTA
                     KNLCHVHHKNGIKYDNHHENLLVLCKDCHRKQPLHEGIFVTQAEMAIIQRLRSQQGLL
                     KAESWNEIYDLTDPSVHGDINMMQHKGFQPPVPGLDLPNSEHEIIATVEAAWPGLKIA
                     VNLTPAEVEGWRIYTVGELVKEIQTGAFTPAKL"
     gene            complement(4272..>4949)
                     /gene="zorB2"
                     /locus_tag="N7B39_004452"
     CDS             complement(4272..>4949)
                     /gene="zorB2"
                     /locus_tag="N7B39_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II Zorya anti-phage system protein ZorB2"
                     /protein_id="HGU2424299.1"
                     /translation="KKDQDNEHWVSMSDLMAGLMMVFMFISIAYMHYVRIEKEKIKEV
                     AVAYENAQLQLYNALDIEFAKDLQDWDAEIDKQTLEVRFKSPDVLFGLGSTELKPKFK
                     LILDDFFPRYLKVLDNYQEHITEVRIEGHTSTDWTGTTNPDIAYFNNMALSQGRTRAV
                     LQYVYDIKNIATHQQWVKSKFAAVGYSSAHPILDKTGKEDPNRSRRVTFKVVTNAELQ
                     IRKIIQE"
BASE COUNT         1365 a         1080 c         1031 g         1473 t
ORIGIN      
        1 aactggtttc aaaatccgaa cgcagtggcg tttacttaac atccacatag ctgacaaatg
       61 ttctttcctc ccgacccact agacctccat aaacatcaaa aggcaggtat ggcacgttaa
      121 tgatttgtgc cgccacgaac actcggtttg attccgtcca gcggatcaaa cttaagatgt
      181 agcccctaga acaaaaaggt cctacgattg ttcttttaaa ggctaatagg aacgtgatta
      241 cagcaggtta tggagactta ggttcgagtc cggccttcgg caccaaaagt atgtaaatag
      301 acctcaactg aggtcttttt ttatgcctga aatccgcgcc acgcaaggct tttccagctt
      361 ttctcctcaa ctgaagtcaa cctgaaccaa cccacatcaa caggcttgtg agtatactaa
      421 tgagtatatt tgccgattcg atattgcttg tatactcacg agataccaat ggaaggaaga
      481 ccattatggc cctaacggat actaaagttc gctctgcgaa acctgaggag aaagagtatt
      541 cacttgttga cggcgacggc atgtccttac ttgtaaaacc tggcggttca aagtattggc
      601 gatttcgttt ccgttttggc ggtaaacaac atttgatggc gttcggcgtt tacccggatg
      661 tttcactggc ggatgcgcgg aagaaaaggg aagaggccag aaagctggta gctgctggta
      721 tcgatcctcg tgagcataag cgtgctgtga aagaagagca ggcgaaagag attattactt
      781 tcgagaaggt tgccagagag tggctcgtaa ccaaccaaaa atggtcggaa gatcatgcta
      841 atcgtgtaaa aaagagcctg gaggacaata tcttcccgac aattggtact cgcaacattg
      901 ctgaactagg cacccgtgat ctgttaattc ctattaaagc cgtggagaaa tccgggcgtc
      961 ttgaagtggc ttcccgcctt caacagcgca ccacggccat catgcgttat gcagtgcaaa
     1021 gcgggttaat tgattacgat ccggcgcaag agatgtcggg ggcggttgct tccagtaatc
     1081 gacagcatcg tccggcgctg gagttaaagc gtatccctga gttgcttgac aaaatagaca
     1141 gctacaccgg cagaccgcta acccactgta cgacagaact cactctgctt atctttattc
     1201 ggtccagtaa gctgcatttt gctcgctggt cagaaatcga ttttgaaacg tcaatgtgga
     1261 ccatccctct tgattggtat tgcagtaagc agagggttgg actggtacag taaatatccc
     1321 gcataatcgt gccattcaca tttagagatc ctccggcata atcaatctgc caatgaagga
     1381 gatcgctatt cgtaaagccc cttttactga gcatcagatt atcgccgtga ttaaatcgct
     1441 tgaatacggg cgaactgtta aagatgtggc ttatctgaag ccacttacta caactgcaag
     1501 tccagatacg gcggtatgga attttctgat attaaaataa tcaaggatct tgaggatgag
     1561 aaccaacgtt tcaaatagat gtttgctgat ctcattattt ggtcgggatt tttaaagatg
     1621 tatggaacta aaacgggtct attttcagag ggatttaact gaagcagaaa ttaataaatc
     1681 ataatttata ggtggctggt atttaatatt cctgcatcat atctctgatg caggagcaga
     1741 gaatggctat aaattattag taaacagaat gtctatctct cacccacaaa cccctcataa
     1801 gggtaatttt tacgcacctg cggcaacgca atagttttcc cgtcaaaatc ccagaataat
     1861 ctctcaacga tccctccgcc gtttaccgca tccgagacca gtacccgcat attttcatcc
     1921 aggccaatcg atcctttatc aaaggcttta tggtgtattg cgcaaagcgc cagaccattg
     1981 gggatttcac aagggccgcc gtgctgtttc cattttatat gcgccgctac cagcgcgacg
     2041 gtggtgtcat cgtggcgcat attaaagcca catattgcgc actggtagtt ataagcgcgt
     2101 aatacatttt tgcgaaataa cgggtcgcgc tgtttacggg tttgctggag atcaaaaccc
     2161 agctcgtcag cgatttcttc ctgaatactc tcggtgaagt gcgcttcgag tatctgttgc
     2221 gccagagtat tgatgagttt tttattgccg gttaccaggg cgtagtgctg ttcatcgaaa
     2281 ccgcctgcaa cgtggtattc gttcagttcc ttcactggcg gttgtcggct gctccctgct
     2341 gttgagcaac gctcggcatt gtgtaattgc cagattccgt cgccttgtaa tcgccagaac
     2401 ggcatatcag gtcggtactg tgaacgctgt ggcccaaaac gttccagtaa actgtgcaga
     2461 ggttcgtaga tttccgtgcc ataatcgaaa aggcgcggat gtccattcag gtatcccgct
     2521 aatacgtata gcagtaacaa tggtttatgc ggcgcgcgct gttcaccctt gtgccagatt
     2581 tttatgttgg caattgcctg ctgtagcgat ttactggaag ccattgatat cccaggcagt
     2641 gaaataattg caatcatgct cgcaaatttt ctgctgttca agcctgcaag tgaagatcca
     2701 ttgctgtgtt gagggatcaa aagacaacaa aaacttattg aagaaataat tttctgcttt
     2761 tttgtcgaaa taatgggcat tgatcttgtc tatagctgct gaatcttttc cagcctcgtt
     2821 gagatcttca aaatagccat tgaagatgtt ctggttaaca ccgccagtaa cataagcctt
     2881 gccgttgtgt acaaaagtca cattacccgc catgcccatc ggcgcgtgcg acatcaattt
     2941 attgctttat tcattgtgac tgtcttctgt ctaccaagct ttaaccttca cccatatgag
     3001 ggaagtttta agtatgaggc gaaccatagc atatgctttt atacctgtta caccataaaa
     3061 atcagacaag ttatgccata aactactttt gtaatttgag ccacaaatca gggaaatttt
     3121 atttatcttc cgtgaaagtc ttaactttca cggagtttta gaatttacaa ttttgctggc
     3181 gtaaaggctc ctgtttgtat ttctttaacc agctcaccca cggtatatat tctccatcct
     3241 tcgacttcgg cgggagtaag gttaactgca attttaaggc ctggccatgc agcttctacg
     3301 gttgcaataa tttcatgttc tgagtttgga agatctaacc caggaacagg aggttgaaag
     3361 cctttatgtt gcatcatatt aatatcacca tgcactgatg gatcagtcag gtcatatatt
     3421 tcattccagg attctgcttt taataaccct tgttgggaac gtaaacgttg aatgatagcc
     3481 atctctgctt gggtaacgaa tataccttcg tggaggggct gttttcgatg gcaatccttg
     3541 cacagaacaa gaaggttttc atggtgatta tcatatttga tgccattttt atgatggaca
     3601 tggcacaagt ttttggcggt tgataaattc acgccacaat cattacaaac ataatttgcc
     3661 ttttctcgta tttctttaga tatttccttc cagttttcga catacccact atttgttttg
     3721 tcatagatac ccttaggcat atgacgaaaa caagtactat aatctgaaaa aaactcattt
     3781 aatgagaatg atttaaaaat ctctcttttt cgttgtttat caatacttcc tttataatta
     3841 agtcgttcga ggcaattcat acatacccgt aaatcaacat cctggctccg acctgaacca
     3901 tcatcaattt cgaataaacc atctatgcgg ttagttgcat gatacctttc aaatcgattt
     3961 ttatggcgca tttcatcaag agttctgcac caggcaatat gaaaacgttt tcctttttcg
     4021 ccatctacga tagccgcatc ataacgccct gaatggtccc gaatatataa aagaacctgg
     4081 cggccatgat aagagataag cccgctacca gggtcaagat cttcgatact aacttcttta
     4141 cccgtggata actcaatatc tataggatca aagtcaatag gggattcatc cagaaaaaaa
     4201 tcgactcctt ctggtaacat tttcttccct aattgaataa gttctgaaat gtcgatagat
     4261 aatttcatct cttactcctg aataatcttt ctaatctgca actcggcatt tgttacaact
     4321 ttgaaggtga cacgacgaga gcgattagga tcctccttgc cggttttatc aagaatggga
     4381 tgtgcagatg aataacctac tgcggcaaat ttacttttaa cccattgttg gtgtgtcgcg
     4441 atatttttta tgtcataaac gtattgtaat actgcacgtg tacgaccttg cgatagtgcc
     4501 atattattaa aataagcaat atcaggattc gttgttcctg tccagtcagt acttgtgtga
     4561 ccttcaatgc ggacttcagt aatatgttcc tgataattat ctagaacttt taggtagcga
     4621 ggaaagaagt cgtcaagaat gagtttaaac tttggtttta gctctgtgct tcctaagcca
     4681 aataaaacat ccggtgattt aaatcgaacc tccagagtct gtttatcgat ctctgcatcc
     4741 cagtcttgta aatcctttgc aaactcaata tccagagcat tataaagctg taactgagca
     4801 ttctcgtagg ctacggcaac ttctttaatt ttttcttttt caatacgtac gtagtgcata
     4861 taagcaatag atatgaacat aaaaaccatc atcagcccag ccattaggtc cgacatggat
     4921 acccaatgtt cattatcttg atctttttt
//
LOCUS       DBFIFI010000266         4947 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.266, whole genome shotgun sequence.
ACCESSION   DBFIFI010000266 DBFIFI010000000
VERSION     DBFIFI010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4947
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.266"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            141..1298
                     /gene="acrE"
                     /locus_tag="N7B39_004453"
     CDS             141..1298
                     /gene="acrE"
                     /locus_tag="N7B39_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HGU2424300.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEVR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            1310..4459
                     /gene="acrF"
                     /locus_tag="N7B39_004454"
                     /pseudo
     CDS             1310..4459
                     /gene="acrF"
                     /locus_tag="N7B39_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
     gene            4712..4933
                     /locus_tag="N7B39_004455"
     CDS             4712..4933
                     /locus_tag="N7B39_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424301.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
BASE COUNT         1200 a         1201 c         1290 g         1256 t
ORIGIN      
        1 ttgagacgcc tgtattcata actattcctt acatcgacga atgataattt gtaggatagc
       61 gaactgtatt tttctttctg cgagttaacg cgttgccttt ttgggtaaat aacgcgcttt
      121 tggttttttg aggaatagta atgacgaaac atgccaggtt tttcctcctg ccctccttta
      181 ttctgatctc cgcggcttta atcgccggtt gtaacgataa gggagaagag aaagctcacg
      241 tcggtgaacc gcaggttacc gttcatattg taaaaacggc cccgttagaa gttaagactg
      301 aattaccagg ccgcaccaat gcttatcgta tagccgaagt tcgcccacag gttagcggga
      361 tcgtactgaa tcgcaatttc actgaaggca gcgatgtgca agcaggccag tccctgtacc
      421 agatcgatcc cgcgacctat caggcaaatt atgacagcgc gaaaggcgaa ctggcgaaaa
      481 gtgaagccgc cgccgccatc gcgcatttga cggtaaaacg ttacgttccg ctcgtgggta
      541 cgaaatacat cagccagcag gagtacgacc aggccattgc tgatgctcgc caggccgatg
      601 ccgccgtgat tgccgcaaaa gccacagtcg aaagcgctcg catcaatctt gcttatacca
      661 aagtgactgc gccaattagc ggacgtatcg gcaaatcgac tgtgaccgaa ggcgctcttg
      721 tcactaatgg gcaaacgact gaactggcaa ctgtccagca gctcgatcct atctatgttg
      781 atgtgaccca atccagcaac gattttatga ggctgaagca gtccgtagag caaggaaatt
      841 tgcataagga aaacgccacc agcaacgtag agttggtcat ggaaaacggt caaacctatc
      901 ccctgaaagg tacgctgcaa ttctccgatg tgaccgttga tgaaagcacc ggctccataa
      961 ccctacgtgc tgtcttccct aacccgcaac atacgctttt gccgggtatg tttgtgcgtg
     1021 cacggattga tgaaggcgtc caacctgacg ccattctcat cccgcaacaa ggcgttagcc
     1081 gcacaccgcg tggtgatgca accgtgctga ttgttaacga taaaagtcag gtggaagtgc
     1141 gccctgtcgt tgccagtcag gcgattggcg ataaatggtt gattagcgaa ggtctgaaat
     1201 ctggcgatca agtcattgtc agcggcctgc aaaaagcgcg tccgggagag caggttaaag
     1261 ccactaccga tacccccgca gatactgcat cgaagtaagg taatctgaca tggcaaactt
     1321 ttttattcga cgaccgatat ttgcatgggt gctggccatt attctgatga tggcgggcgc
     1381 actggcgatc ctacaattgc ccgtcgctca gtatccaacc attgcaccgc ctgcggtttc
     1441 tgtttcagca aactatccgg gcgctgatgc gcagaccgtg caggatacgg tgacgcaggt
     1501 tatcgaacag aatatgaacg gtatcgataa cctgatgtat atgtcctcca ccagcgattc
     1561 cgccggtagc gtgacaatta cccttacctt ccagtccggg accgatcctg atatcgcgca
     1621 agtgcaggtg cagaacaaac tccagctcgc cacgccgttg ctgccgcagg aggttcagca
     1681 gcaggggatc agtgttgaaa agtccagtag cagctatttg atggtggcag gctttgtctc
     1741 tgataaccca gacaccacac aggacgatat ctcggactat gtggcctcta acgttaaaga
     1801 tacgcttcgc cgtctgaata gcgtcggtga cgtacagctt ttcggcgcac agtatgcgat
     1861 gcgtatctgg ctggacgccg atctgctaaa caaatataaa ctgacaccgg ttgatgtgat
     1921 taaccagttg aaggtacaga acgctcagat cgctgccgga cagttgggcg gaacgccagc
     1981 gttaccaggg caacagttga atgcctcgat tattgctcag acgcggttaa aaaatccgga
     2041 agaattcggc aaagtgaccc tgcgcgtaaa cagtgacggc tcggtggtgc gtctgaaaga
     2101 tgtcgcacgg gttgaacttg gcggtgaaaa ctataacgtt atcgctcgca tcaacggaaa
     2161 accggcggcg ggcctgggta ttaagctggc aaccggcgcg aatgctctcg ataccgcgaa
     2221 agccattaag gcaaaactgg cggaattaca gccattcttc ccgcagggaa tgaaggttct
     2281 ctacccttat gacaccacgc cattcgtcca gctttctatt cacgaagtgg taaaaacact
     2341 gttcgaagct attatgctgg tgttcctggt gatgtatctg ttcttgcaga atatgcgagc
     2401 aacgctgatc cccaccattg cggtacccgt ggtgttgtta gggacgtttg ccatcctcgc
     2461 cgcttttggt tactccatca acacactaac gatgttcggg atggtgcttg ccatcgggct
     2521 gctcgtcgat gatgcgatag tggtggtgga gaacgtcgag cgcgtgatga tggaggataa
     2581 gctcccgcca aaagaagcga cggaaaaatc aatgtcgcaa atacagggcg cactggtggg
     2641 tatcgcgatg gtgctgtcag cggtatttat tccgatggca ttcttcggcg gttctactgg
     2701 ggcaatttat cgtcagttct ctatcaccat cgtttcggca atggcgcttt ctgttctggt
     2761 ggcattgatt cttacccctg cgttatgtgc aacactgctc aaacccacct cagctgagca
     2821 tcacgaaaat aagggtggtt tcttcggctg gtttaatacc accttcgatc atagcgttaa
     2881 ccactacacc aacagcgtcg gcaaaatcct cggttccaca ggacgatatt tactgatcta
     2941 tgcgctgatt gttgcaggaa tggtggtgtt gtttttacgt cttccgtctt ccttcttacc
     3001 tgaagaggat cagggtgtct ttctgaccat gattcagtta cccgctggcg cgacgcaaga
     3061 gcggacgcaa aaagtgttgg atcaagtgac ggattactat ctgaagaacg agaaagcaaa
     3121 cgttgaaagt atctttacgg ttaacggctt tagcttcagc ggccaggcac ctccgcaacc
     3181 ccatgttttc acataactgt tgcgttgacc aattgaatct acaggtatgg ccttcgtcag
     3241 tctgaaaccg tgggaagagc gtaatggtga tgaaaacagt gcggaagcgg taatccatcg
     3301 tgccaaaatg gagttgggca agatccgcga cggttttgtc attccattca atatgccagc
     3361 cattgttgaa ctgggcacgg caacgggttt cgactttgag ttaattgata aggctgggct
     3421 gggtcacgat gccctaaccc aggcccgtaa ccagttgctt ggtatggcgg cgcaacatcc
     3481 tgccagctta gtcagcgtgc gtcccaatgg cctggaagac accgcgcagt ttaaactgga
     3541 agttgaccag gaaaaggcgc aggcattagg tgtttcactt tctgacatca atcagaccat
     3601 ttcaacggcg ttgggtggga cttacgttaa cgacttcatc gaccgtggtc gcgtgaaaaa
     3661 ggtgtatgtt caggcggatg ccaaattccg tatgctgcca gaagatgtcg ataaacttta
     3721 tgtccgcagc accaacggcg aaatggtgcc attctctgcc tttaccactt cacattgggt
     3781 gtatggctct ccgcgactgg aacgctacaa cggtctgccg tcaatggaga ttcaggggga
     3841 agccgcgcca ggaaccagtt ccggcgatgc catggcgttg atggaaaacc tagcgtcaaa
     3901 attacctgcg ggcattggtt atgactggac gggtatgtcg tatcaggaac gcttatcggg
     3961 aaaccaggct cccgctctgg tagcaatttc ctttgtggtt gttttcctgt gccttgctgc
     4021 actctatgaa agctggtcaa ttcctgtctc ggttatgttg gtcgtgccgt tagggattgt
     4081 cggcgtgctg ctggcggcga cactctttaa tcaaaaaaat gacgtctact ttatggtagg
     4141 cttgctaacg acaattggct tgtcggccaa aaacgctatt ttgattgttg agttcgctaa
     4201 agatctcatg gagaaagagg gtaaaggtgt tgttgaagcg acactaatgg cagtacgtat
     4261 gcgtctgcgt cctatcctga tgacctctct cgcctttatt ctcggcgtat taccgctggc
     4321 tatcagtaac ggtgccggca gtggcgcgca gaacgcagtg ggtatcgggg taatgggagg
     4381 aatggtctct gcaacgttgc tggcaatctt cttcgtaccg gtgttctttg tggtgatccg
     4441 ccgttgcttt aaaggataaa tctgaaacat aaaggcgctt tcgggtgcct ttatgttttc
     4501 cagtgaaacc cataaaaatt aataagatat tcttttgctc actctttaaa agctttctat
     4561 agctcccgct cccttcacta tttttacaat tcacataatt tgtcattatt cattccgcaa
     4621 gatttataac ctccggtgat aaaatggcat tcagctcgtt aataagagag ttaacttatt
     4681 aagcgttagc gtttattact gaggtaacac catgaaaaga ttaattcctg tggcattgct
     4741 caccgcattg ctggcgggct gtgctcacga ttcgccctgc gttccggttt atgacgatca
     4801 gggtcgtctg gttcacacca atacctgtat gaaaggcacg actcaggata actgggaaac
     4861 ggctggggct attgctggcg gggcagcggc tgttgctggc ctgacgatgg ggatcatcgc
     4921 tctgtcgaaa taactgaaca gggcgcg
//
LOCUS       DBFIFI010000267         4899 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.267, whole genome shotgun sequence.
ACCESSION   DBFIFI010000267 DBFIFI010000000
VERSION     DBFIFI010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4899
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.267"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..3212)
                     /locus_tag="N7B39_004456"
     CDS             complement(<1..3212)
                     /locus_tag="N7B39_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HGU2424302.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQKVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGASFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAILRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAGYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDLSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSETRIADIRQVETSARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGRASDDAEGYLDFFKGKITESHLGKELLEKVDLTEDNASRLDEFSKEWKDAN
                     DKWNAMWGVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETP
                     MFVAQGNQIFMNDVFLKRLTAPTITSGGNPPAFSLTPDGKLTAKNADISGSVNANSGT
                     LNNVTINENCQIKGKLSANQIEGDIVKTVSKSFPRTSTYASGTITVRISDDQKFDRQV
                     MIPPVLFRGGKHENFNSNNQQSYWYSTCRLRVTRNGQEIF"
     gene            complement(3273..3845)
                     /locus_tag="N7B39_004457"
     CDS             complement(3273..3845)
                     /locus_tag="N7B39_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HGU2424303.1"
                     /translation="MKTGAEAIRALATQLPVFRQKLSDGWYQVRITGRDVSTSGLTAQ
                     LHETLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGM
                     TGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMVAQGNVLPVLY
                     GEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            complement(3842..4585)
                     /locus_tag="N7B39_004458"
     CDS             complement(3842..4585)
                     /locus_tag="N7B39_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HGU2424304.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVSTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWRASAFTGICNDLAAASTFV"
     gene            complement(4591..>4899)
                     /locus_tag="N7B39_004459"
     CDS             complement(4591..>4899)
                     /locus_tag="N7B39_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HGU2424305.1"
                     /translation="VISRWRIEQCSELSAVSASFVLSTPTETDGAVFPGRIMLANTCT
                     WTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNVGNFGGFLSINKLSQ
                     "
BASE COUNT         1007 a         1542 c         1253 g         1097 t
ORIGIN      
        1 ttaaaaatct cctggccatt gcgggtcact cttaaccggc aggttgaata ccagtatgac
       61 tgttggttat tactgttgaa attctcatgc ttaccaccgc ggaataacac tggcggtatc
      121 atgacctgcc ggtcaaattt ctgatcatca ctgattctta ccgtgatggt gccactggca
      181 taagtgctcg tgcgggggaa agacttgctg accgttttga caatatcgcc ttcaatctga
      241 ttggctgaca gtttcccctt aatctgacag ttctcattaa tcgtgacgtt gttgagcgtc
      301 ccggagttcg cattcacact gccactgata tccgcatttt tagcagtcag ctttccgtcc
      361 ggtgtcaggg aaaatgccgg tggatttcca ccgctggtaa tggtgggggc cgtcaggcgc
      421 ttcaggaaca cgtcgttcat gaatatctgg ttgccctgcg ccacaaacat cggcgtttca
      481 ttcccgtttg ccgggtcaat aaacgcgata cgattggcgg caaccagaaa ctggctcagc
      541 ttgccttcct ccgtgtcctc catgctgagg ccaatacccg cgacataatg tttgccgtct
      601 ttggtctgct caattttgac gccccacatg gcattccact tatcgttagc atccttccac
      661 tctttcgaaa actcatccag tctgctggcg ttatcctccg tcaggtcaac tttttccagt
      721 agctctttac caagatgaga ttcggttatc ttgcctttga aaaaatccag ataaccttcc
      781 gcatcatcgc tcgcccgacc gacggcctcc acgaatgccg atttgccaac ggtgttcaca
      841 ctgcggatat aaaagtaata atcatggccc ggtttgatat tgatactggc ggctatccag
      901 tacagcgccg taccaagata acgcgcgctg gtttcaacct gcctgatatc cgcaatccgc
      961 gtttccgaga accagaactc aaactgtacc gtcggatcat aaaccgcaag atgcggcgtg
     1021 gcggttatct gaaaatagcc tggtgtcagc tcaatccgag acggcgctgc cggtgcggca
     1081 atccggaacg ataccgatgc cggatcgccc tgctgtcccc acgcatttac cgcccggaca
     1141 gtcagcctgt agttccccag cgccagttgc gtgaagcggt atgtggtttc cgtcgtcctg
     1201 gccgtactga ccagccgctc actgccatcg tccgctgcca cggtcaggcg aagcaggaag
     1261 ctcaccccct tcaccacctt cggcgtgtcc cagcgcgcca gcacctgata ttccccgctg
     1321 tctgcggtga cttcggcagt caggtgctgc accgcgggcg gcgtgacgcc attcaccgtg
     1381 ccgctcaggt cgccgtcaaa gtgcgccccg ttatccacga tggcttcttt ttcgggtaca
     1441 tgctgcacgg cggtgatggc atacgtgccg tcgtcgttct cacggatact cacgcagcgg
     1501 aacaggcgct ggcgcaacgt cggcaacttc agcccccata cgctgtatcc ggcaacgccg
     1561 tcaggaacac ggctcacttt taccttcacg ccgtcggtga cggactggac ctccacgctg
     1621 accggattgc cacttccgtc aaccaggctt atcagcgtgg taccggagga tggcagcgtg
     1681 atttcacggt cgagcgtcag cgtccgggtc tggctgttca ccgccagcac acgcccaccg
     1741 atgctgatcc ccgcatagtc atcatcacag atttcaatga catcgcccgg tacatggcga
     1801 agcccttctg cgcccacgct gaaatccacg gtctgcgtct ccagcagctc cgttttaatc
     1861 agccacagcc cggcgcggtg tgcctgcccc cggctggtac agccaaaggc atccatcttc
     1921 gtgacgttac gaccgtaacg gagaatggcc tgcgtgtcct ccacaagctc tgtcgccgtc
     1981 tcccagccgt tatccgggtc aatccagttc acctcaacgg cattatggcg gtccttgagg
     2041 gcgctgaagc tgtagcggaa cgacgcgcca tcatccggca tcaccacatt actgcggtta
     2101 taggtccaca ccttatccga cggtcggtcc tgcacgaacg tcagcgtctg cccgttccat
     2161 accggcatac agcgcatcgc cgagcagaaa tcgctgagaa catcccacgc cttacgctgt
     2221 gtggtcaggt aggcattaca ggtgatgcgc ggctccgtgc cgccaaagcc gtccggcact
     2281 gactggtcgc agtactggcc gatgacatac agcgcccatt tatccacatc cgccgcacca
     2341 agacgtttcc ccatgccgta gcgcggatgg gtcagcatat cccacagaca ccaggccatg
     2401 ttgttgctgt atgccggttt aaacgttccg tcccagatac cgctgtattg ccgcgtctgc
     2461 gggttataat tcgacggcac ctgcagaata cgcccgcgca gatgataatt acggctcacc
     2521 ttctggctgc cgaactgctc cgagtccacc tgcacgccga ccagtgccgt gttcgggtag
     2581 cactgtttca catcgataat ttcggtgtat gacgaccaga gcgttttgtt ctgcagctgg
     2641 tctgtggtgc tgtccggcgt catcctgcgc atccggatat taaacgggcg cggcggcagg
     2701 ttatccacca ccaccgaggc cagatactgt gaagtggttt tacccttaat ggtgatgtct
     2761 ttttccgtca cccagccacc gttacgttgt atctgaacca gcaggcggac ttccgatgga
     2821 ttcctgtccc cctttgaggt ggtttccacc agtgcctgca cgccgaaagt aaaacgcaga
     2881 cggtcaatgt ttgccgacgt gatggtccgg gtgatcggcg tgtcatattt cacttctgta
     2941 ccgagcaccg tctcggagcc ggaggattca aatccctccg gcggtgtctg ctcctgctca
     3001 ccggcccgga acaccaccgt gacgccggat atattggtat tcccctcact gtccagcacc
     3061 ggcgtactgt tcagcagcac gctttttaat ccatccaccg gaccttcaac cggcccttcg
     3121 ctgatggcat cgatcacact cagcagctgc gtggacttca ggttgtcctt cgcttcgcgc
     3181 ggggtatgcc ccttactgct gcctttaccc attcgtcatg ctccataaac gataaaaccg
     3241 cccggaggcg gtttcacata aaacattttg catcagcgac caatcaccac aacctgacca
     3301 ccgtcccctt cgtctgccgt gctgatctct tgagaaacca cgcgtgaccc cacgcgcatt
     3361 tcaccgtaca gaaccggcag aacattgccc tgggcaacca tgttatccag tgaggagaaa
     3421 taggtgttct gtttgccgtt atccgttgtc tgtgtacggg gagttctggc tttcggtgcc
     3481 agcatctgcg ccacaccacc gagcaccata ctggcaccga gagaaaacag gatgccggtc
     3541 ataccaccgg ccccaatggc tgccccccat gctgcaaggg tggctccggc ggtaaagaat
     3601 gatccggcaa tggcggcagc ccccaggaca atctggaata cgccacctga cttggccccg
     3661 gcgactctgg gaacaatatg aatcacagcg ccatcaggca gagtctcatg taactgcgcc
     3721 gttaacccgg acgtgctgac gtcccgcccg gtaatccgta cctgatacca gccgtcgctc
     3781 agcttctgac gaaacaccgg gagctgtgtg gccagtgcgc ggatggcttc agcccccgtt
     3841 ttcacacgaa ggtcgatgcg gcggccaaat cgttgcaaat ccccgtaaag gcagatgcgc
     3901 gccatgcccg gtgacgccag agggagtgtg tgcgtcgctg ccatttgtcg gtatacctct
     3961 ctcgtttgct cagttgttca ggaatatggt gcagcagctc gccatcacca cagtaaatgg
     4021 cggcatgatt cggcaccgat gaaccaaaac agcacagcag cacatcgccc ggttgtgctg
     4081 atgacaacgg cacctgatac agccctgtgg cctccagatt atccagatag agattctgac
     4141 cgtgacgcca ccagtcatcc tcgcgatgaa aatccggcat ctcaatcccc gccagatgat
     4201 aagcatcccg gaacagcgtg taacagtccg tcaccccgtg ctcaaagcgc cgcccggtga
     4261 gatgcggcac acagcggaac ttatgaatcg tcccccggca gaccagccac cacggcaaat
     4321 cactctgcac ctgcagccgc cggtcggcct cactcagcca gggcagacca ccggggtggc
     4381 tgtggaccag cgccacaatc tcaccctgca tttctgcctg cagccagtct tccggcgaca
     4441 tacggaaata cgcctccggc tcaccggaga tattcacgca ggggaaatat ctttccccct
     4501 ccggcgtgct taccacgaag ccgcacgact ccgctggcgc acatcgccgg gcgtgcgcca
     4561 gaatcgctga ttctgtctgt gtcatgggat ttactgcgaa agtttgttaa tggaaaggaa
     4621 gccgccaaag ttgccgacgt tattgcggaa cttacaaccg ctcaggcatt tgctgcattt
     4681 atccttcgtg atatcggacg ttggctggtc atattcatcc gcgaccgccg gaccgtgata
     4741 accgcactca tcgccgcgat aggtccaggt gcaggtgttg gccagcatga tacgtcccgg
     4801 aaaaacagcg ccgtccgttt ccgtcggcgt ggacagtaca aaagaggcac tcaccgcgct
     4861 cagttcgctg cactgctcaa tgcgccagcg gctgatcac
//
LOCUS       DBFIFI010000268         4894 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.268, whole genome shotgun sequence.
ACCESSION   DBFIFI010000268 DBFIFI010000000
VERSION     DBFIFI010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4894
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.268"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..88)
                     /locus_tag="N7B39_004460"
     CDS             complement(<1..88)
                     /locus_tag="N7B39_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HGU2424306.1"
                     /translation="MEAKECKVQDILTENKKFIIPSYQRPYSW"
     gene            366..2798
                     /gene="hsdR"
                     /locus_tag="N7B39_004461"
     CDS             366..2798
                     /gene="hsdR"
                     /locus_tag="N7B39_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EcoAI/FtnUII family type I restriction enzme
                     subunit R"
                     /protein_id="HGU2424307.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPEQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVKDTDDVGPLPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVRKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2865..4334
                     /locus_tag="N7B39_004462"
     CDS             2865..4334
                     /locus_tag="N7B39_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HGU2424308.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSRDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPAELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4334..>4894
                     /locus_tag="N7B39_004463"
     CDS             4334..>4894
                     /locus_tag="N7B39_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGU2424309.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASELLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVRISEIGHDWGQKTPDKDFTYIDVGSINKEYGIIEELSILSAKDAPSRARKIV
                     QQGTIIYSTVRPYLLNIAIIENEILPE"
BASE COUNT         1435 a         1179 c         1169 g         1111 t
ORIGIN      
        1 tccaactgta aggacgctga taagaaggaa taataaactt tttgttttca gtaaggatat
       61 cctgcacctt acattcttta gcttccattt catataccca atcaaacaat caacaataac
      121 attatctttc ttgctcgaaa gaaacgcaac tataagattt ttatatattt tgttagagtt
      181 gatatggttc ctgtaacttc tgtttatctg tctctgatat gcccagatta attcaagcaa
      241 gatataacag agatatcaat gtgatctctg ctacgtactt tacgttgaaa caccgcccta
      301 aatcatagcc aggctattga ttttatgtac aatcagaatc gtcctgataa aagcgagaat
      361 accccatggc ggaactgaac ctaagtaacc tgacggaagc agacatcatt accaaatgcg
      421 ttatgccagc cattctcaat gcaggctggg acaacacaac gcaaatcaga caggaggtca
      481 aactccggga cggtaaagtc atcgtacgtg gtaaagttgc ggcacgcaga acggtaaaat
      541 ctgctgacat cgtgctgtat cacaaacctg gcattccctt agccgtgatt gaagcaaaag
      601 ccaacaaaca tgaaattggc aaagggatgc aacagggcat tgaatatgcg cgcctgctgg
      661 acgttccttt tgtttttgcc accaacggtg atggctttat cttccgcgat gccaccgcag
      721 ccgaaggtga atgccttgaa aagcaaataa cgctggatga cttcccctcc cccgctgaac
      781 tctggcaaaa attctgcctc tggaaaggat acacacaagc tcagcttccg gtgattactc
      841 aggactatta cgacgatggc agcggtaaat cgccacgtta ttaccagctt caggcaatca
      901 acaaaaccat tgaagccgtc tccaacgggc aaaaccgcgt tctgctggtc atggcgaccg
      961 gaacagggaa aacctatacc gcattccaga tcatctggcg cctgtggaaa tcaaaaaata
     1021 aaaaacgcat tttgttcctt gccgatcgca atattctggt cgaccaaacc aaaaataatg
     1081 atttccagcc atttggtacg gcaatgacca aagtcagcgg acgcaccatt gatcccgctt
     1141 atgaaattca cctcgcgctc tatcaggcta taactggccc ggaggaagac caaaaagcgt
     1201 ttaaacaagt cgcaccagat ttcttcgatc tgatcgtgat cgacgaatgc catcgcggca
     1261 gcgcatccga agacagcgcc tggcgagaaa tccttgatta ttttagttcc gccacccaaa
     1321 ttggcttaac cgccacgcca aaagagacgc atgaagtctc cagcacggat tacttcggcg
     1381 atccggttta cgtctactca ctaaaagaag ggatcgaaga cggcttcctc gccccttata
     1441 aagttgtccg tgttgatatt gatgttgatc tgcaaggctg gcgcccaacc aaagggcaaa
     1501 ctgacttaaa cggcgaagtg atcgacgatc gtatctataa ccagaaagat ttcgatcgca
     1561 cgatggtaat cgacgaacgc acagaactgg ttgccagaac cattaccgac tacctcaagc
     1621 gtaccaatcc gatggataaa accatcgtct tctgtaacga catcgatcat gcagaacgta
     1681 tgcgccgcgc cctggttaat ctcaacccgg agcaggtgaa aaagaacgac aaatacgtca
     1741 tgaaaatcac cggcgatgat gaaattggca aagctcagtt ggataacttc atcaacccga
     1801 aaaaaccgta cccggttatc gcgaccactt cagagctgat gaccaccggt gtggatgcta
     1861 aaacctgcaa actggtagta ctggaccaga acatccagtc gatgaccaaa ttcaagcaga
     1921 ttatcggtcg tggtacacgc atcgacgaac gttacggcaa actctggttt accatcctcg
     1981 actttaaaaa agccaccgaa ctgtttgccg atgagcgttt cgatggcatt cccgaaaaag
     2041 tcatggatac cacaccagag gatatcgccg atccagaatc tgattttgaa gagaaactcg
     2101 aagaaatcag cgaacatgac gacgaacagg taacaggcgt tgatgaaccg cctgcgccac
     2161 cataccaggt taaagatacc gatgatgtcg gcccacttcc ggaagaagac gagaagaaaa
     2221 tccgcaagtt tcacgtcaac ggtgtagcag tgggcgttat tgcccagcgt gttcagtatt
     2281 acgacgccga cggtaaactg gttaccgaat cctttaaaga ttacacccgc aaaacactgc
     2341 tcaaagaata tgcctcgctg gatgacttta cccgcaagtg gcaggacgcc gatcgcaaag
     2401 aagcgatcat tcacgagctg gagcaacagg ggatcatctg ggaagtactg gcagaagaag
     2461 tcggtaaaga tctcgacccg ttcgacatgc tttgccacgt agtgtatggt cagccgccgt
     2521 taacccgcaa agagcgcgcc gagaacgtgc gcaagcggaa ctacttcaca aaatactctg
     2581 aagcagcgca agccgtgctc gataatctgc tggataaata cgccgatgcg ggcgtacagg
     2641 agatcgaaag tattcaggtg ctgaaactta agccattcga cagcatgggc accttaccgg
     2701 agattattaa aaccggattt ggcgaccgta acgggtataa tcaggcgctc agcgagctgg
     2761 aaaacgaaat ctaccaatta ccgccccgct ctgcttaaca actctctttt gcgccttctg
     2821 caaccatcat gttgcagaag gccttgtttt tcatggaata caacatgtct atcagctcag
     2881 taatcaaatc attacaagat attatgcgca aagatgccgg tgtggatggc gatgcgcagc
     2941 gtctcggtca gctctcctgg ctgctgtttt tgaaaatctt cgatgcccag gaagaggcgc
     3001 tggaactgga gcaggataac tatcaatatc cgatcccaca gcgttattta tggcgcagtt
     3061 gggccgccaa cgcgcagggc attaccggtg attccctgct ggaattcgtt aatgatgatc
     3121 tgttcccggc gttgaaaaac ctcactgcgc ctatcgataa aaacccacgc ggctacgtgg
     3181 tgaagcaggc gttcagcgat gcctataact atatgaaaaa cggtacgcta ctgcgtcagg
     3241 tgatcaataa gctgaacgaa attgacttta ccagcgccag cgaacgccat ctgtttggtg
     3301 atatttacga acagatcctt aaagatctgc aatctgcggg caatgcgggc gaattctata
     3361 ctccacgcgc cgtcactcgc tttatggtgg atcgcgttga tccgaaactc ggcgaatcca
     3421 ttatggaccc ggcctgcggt acgggcggtt ttcttgcctg cgcatttgat catgtaaaga
     3481 acaaatacgt gaagagcgtc gccgatcatc agacgctgca acaacagatc cacggtgttg
     3541 agaaaaaaca gcttccgcac ctgctggcga ccaccaatat gctgctacac ggcattgaag
     3601 tgccagtgca aattcgtcac gacaacactc tgaacaaacc gctttcctcc cgggatgagc
     3661 aactggatgt cattgttacc aacccgccgt ttggtggcac ggaagaagac ggtattgaga
     3721 agaactttcc ggcagagatg caaacccgcg aaacggcgga tttgttcctg caactgattg
     3781 tggaagtact ggcgaaaaat ggtcgcgcgg cggtggtatt gccggatggc acactatttg
     3841 gcgaaggcgt taaaaccaaa atcaaaaagc tacttaccga agagtgcaac ctgcatacca
     3901 tcgtgcgctt accgaatggt gtgtttaacc cctataccgg cattaaaacc aacctgttgt
     3961 tctttaccaa aggtcagcca accaaagaga tttggttcta tgagcatccg tatccggcgg
     4021 gcgtaaaaaa ctacagcaaa actaagccga tgaagtttga agagtttcag gcggagatcg
     4081 actggtgggg taacgaggcc gatggttttg ccagccgcgt agagaatgag caggcgtgga
     4141 aagtcagcat tgatgacgtg attgcgcgta acttcaatct ggatattaaa aacccacatc
     4201 aggcggaaac cgtcagccat gatccggccg agctgttagc gcagtatgca aaacagcagg
     4261 cggagatcca gacgctgcgt aatcaactgc gcgatattct tggcgctgcg ctgtctggca
     4321 aggaggttaa ctaatgagtg tggaaaagct gatcgttgat catatggaaa cctggacctc
     4381 ggcgttgcaa acccgttcca ccgccgggcg cggcagttcc ggaaaaattg atttatatgg
     4441 cattaagaaa ttacgtgagc tgattctgga actggctgtg cgcggtaaac tggtgccgca
     4501 ggacccgaac gatgaaccgg cgtcggagct gctgaagcgt attgcggcag aaaaagcaga
     4561 gctggtgaag caaggtaaaa ttaaaaagca aaaaccactg ccggaaatta gtgaggaaga
     4621 gaagccgttt gaattgccgg agggatggga gtgggtgcgt attagtgaaa ttggacatga
     4681 ctggggacaa aaaactccag ataaagattt tacttacatt gatgttggtt caataaataa
     4741 agaatatgga attattgaag agctatctat tctttcagcg aaagatgccc catcgcgagc
     4801 gagaaaaatt gttcagcaag ggactatcat atactctact gttcgcccgt atttgttaaa
     4861 tatagctatt attgaaaacg aaatactacc tgaa
//
LOCUS       DBFIFI010000269         4830 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.269, whole genome shotgun sequence.
ACCESSION   DBFIFI010000269 DBFIFI010000000
VERSION     DBFIFI010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4830
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.269"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..926
                     /gene="ybeF"
                     /locus_tag="N7B39_004464"
     CDS             <1..926
                     /gene="ybeF"
                     /locus_tag="N7B39_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HGU2424310.1"
                     /translation="LSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHKGIVNAAKVLN
                     LTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLESILGALDIEGS
                     YDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQLSQFQTDLIID
                     NMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLLPEGQNFSGLR
                     QRIQEMFPDRQISFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWPLEKLPFPSLN
                     EEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            1135..2100
                     /gene="lipA"
                     /locus_tag="N7B39_004465"
     CDS             1135..2100
                     /gene="lipA"
                     /locus_tag="N7B39_004465"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HGU2424311.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(2201..2404)
                     /gene="tatE"
                     /locus_tag="N7B39_004466"
     CDS             complement(2201..2404)
                     /gene="tatE"
                     /locus_tag="N7B39_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HGU2424312.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(2533..3321)
                     /locus_tag="N7B39_004467"
     CDS             complement(2533..3321)
                     /locus_tag="N7B39_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HGU2424313.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            3414..3797
                     /gene="crcB"
                     /locus_tag="N7B39_004468"
     CDS             3414..3797
                     /gene="crcB"
                     /locus_tag="N7B39_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HGU2424314.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(3851..4060)
                     /gene="cspE"
                     /locus_tag="N7B39_004469"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(3851..4060)
                     /gene="cspE"
                     /locus_tag="N7B39_004469"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HGU2424315.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(4235..4795)
                     /gene="pagP"
                     /locus_tag="N7B39_004470"
     CDS             complement(4235..4795)
                     /gene="pagP"
                     /locus_tag="N7B39_004470"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HGU2424316.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIVQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT         1282 a         1227 c         1083 g         1238 t
ORIGIN      
        1 gtttaagcag aaaatcgtca gaaggtaaac cacaaatatt tacaacctta cgaaatattg
       61 atcttaatct tctgactatt tttgaagctg tatatgtaca taaagggatc gttaatgcag
      121 cgaaagtgct taatctgacc ccctcggcaa tcagtcagtc tattcagaaa ctgcgcgtta
      181 tattccctga cccattgttt attcgcaaag gccagggtgt cactcctacc gcatttgcga
      241 tgcatctaca tgagtatatc agtcagggcc ttgagtccat tcttggcgcg ctggatatcg
      301 aaggaagcta tgataagcaa cgaacgataa ctattgcgac caccccctcg gtcggagctc
      361 tggtccttcc tgtcatttat cgggcgatta aaactcacta tccgcagctt ttactgcgca
      421 acccgcccat cagcgacgcg gaaaaccaac tcagtcagtt tcaaaccgat ctcatcatag
      481 ataacatgtt ttgcaccaat cgtacggtgc aacatcatgt tctgttcacc gacaatatgg
      541 tgttaatttg ccgcgaggga aatccactac tctctttaga agatgacaga gagactatcg
      601 acaacgctgc acatgtactc ctgttaccgg aaggacaaaa tttcagcggt ctgcggcaga
      661 gaattcaaga gatgtttccg gaccggcaga tcagcttcac cagctacaac attttgacaa
      721 tcgctgcact ggttgccaac agtgacatgt tagcgattat tcccagccgt ttttataacc
      781 tgtttagccg ttgctggccg ctggaaaaac tgccttttcc gtccttaaat gaggagcaaa
      841 tagatttctc tatccactac aacaaattca gcctgcgtga tcccatcctg cacggagtaa
      901 tagatgttat ccgtaatgca ttttgaaaaa gtattgttac agccagatca cagcgcagaa
      961 cgataaaatg gcacaatcca acaacaattt tacattttcg cgaccgcttt ggctgctttc
     1021 aggtccgttt caatgatata ctgccagtcg ttaattcaaa aatagttgat aattacaaca
     1081 atctattgaa ttgaaacgct ttccttcgta attcgcaact ggaacacgca cgctatgagt
     1141 aaacccattg tgatggaacg cggtgttaaa taccgcgatg ccgataagat ggcccttatc
     1201 ccggttaaaa acgtggcaac agagcgcgaa gccctgctgc gcaagccgga atggatgaaa
     1261 atcaagcttc cagcggactc tacacgtatc cagggcatca aagccgcaat gcgcaaaaat
     1321 ggcctgcatt ctgtctgcga ggaagcctcc tgccctaacc tggcggaatg cttcaaccac
     1381 ggcacagcaa cgtttatgat cctgggcgct atttgtaccc gccgttgtcc gttctgtgac
     1441 gttgcccacg gtcgcccggt agctcctgat gccaatgagc cagtgaaact ggcgcagacc
     1501 attgccgata tggcgctgcg ttatgtggtt atcacctccg ttgaccgtga tgacctgcgc
     1561 gatggcggtg cccaacactt tgcggattgt attactgcca ttcgggaaaa aagcccgcaa
     1621 atcaaaattg aaactctggt gccggatttc cgcggtcgta tggatcgtgc tctggatatt
     1681 ctgactgcaa cgcccccaga tgtgttcaac cataacctgg aaaacgtacc gcgtatttac
     1741 cggcaggtac ggcctggcgc agattacaac tggtcgctga agctgctgga acgctttaaa
     1801 gaagcgcatc cggaaatccc gaccaagtct ggtctgatgg tgggtctggg tgaaaccaat
     1861 gaagaaatta ttgaggtaat gcgcgacctg cgccgtcatg gtgtgacgat gttaacgctg
     1921 gggcaatatt tgcagccaag ccgccatcac ctgccggttc aacgttacgt tagcccggat
     1981 gagttcgacg aaatgaaagc cgaagcgctg gcgatgggct ttacccatgc tgcatgcggt
     2041 ccgtttgtcc gctcttctta ccacgccgat ttgcaggcga aagggatgga agttaagtaa
     2101 tcctgtaata attacttaca tctgtctgat aaaaaacccg ctttttgaag cgggtttttt
     2161 gtatcgaaca agatattgag ggagcgtcct gctcgccacg tcactcttta tgagagagct
     2221 tttcagcctg aagatcaacg tctgcgcctt ttttcgccgc agcatcgtca tcattcatcg
     2281 ccttcttgaa ccctttaatg gccgctccaa ggtctccgcc cagcgtacgt aacttcttag
     2341 tcccaaacag cagaacgacc agcgccgcaa ctaccagcag tttggtaata ctaatctcac
     2401 ccatagatac cttcttgaca tataaccggc aacagaacgc gccttaaaaa ccaaagaata
     2461 ttaacggtca ttcgacgcgc gcacacaata gcaatctgta acaaggtgaa tcaagcgccg
     2521 agtaaaaaaa catcataata attgcggcgg cgcaaagcga cggttgttta agacgggcag
     2581 ttgcgcgcgc acctgacgca cacgttcggg cgtcacttcc gccataatga gtgcaggcat
     2641 ttctgacgct gccgcaatgg tgacgccaaa gggatctata atccggcttt gcccgatatt
     2701 tttgttcccg cactcccccg ccgccaccat ataacaggtg gtatccagcg cacgagcggc
     2761 aagcaacgtt gaccagtggt gctctttgag cggcccgcga acccaggcgg caggaagtac
     2821 caggatttca gctccctgta atgcctgtgc cagcgccagc tctggaaagc gtaagtcata
     2881 acaggtcatc agaccgacct tcatcccctc cacctcgagt aacggagcaa tttcattacc
     2941 agcatcaaca cggcgtgatt cctgaatggc aaatgcatca tagagatgca atttggcata
     3001 acgggcgacg atgtttcctg cctgtagcgc caccagcata ttccatgcgc gccccggcgt
     3061 tgaaggaaca tgaatcgtca gaattgtcgt catcatgtta cgtttacttt ctcgccgtaa
     3121 acgtccgagg aattcgcctt ccagcagctg tgctgattta accgatagat ctgcatcatg
     3181 atcatcgcgc gccagcaatg cttccggcag gacaaacagc gatacgtcgt tttccgccgc
     3241 ctgcgccatc aacgaggcac aaatctcagc gttcttttcc cacacagatg taacagcaaa
     3301 ctgtcctgca gcaaccagca ttgccacctc ctgtcagagt cgtaaaattc gccaacgacg
     3361 attattcggc gttacactta tcactcatac aaatcaaata gcaggatttt gcagtgttac
     3421 aacttctttt agcagttttt attggcggtg gtacgggaag cgtggcgaga tggctgttaa
     3481 gtatgcgatt taacccgctg catcaggcga ttccgttggg gacgctggca gcaaacctga
     3541 ttggggcatt catcatagga atgggattcg cctggttcag caggatgacg aacattgatc
     3601 cagtgtggaa agtattaatc accaccggat tttgtggcgg tctaacaacc ttctcaacat
     3661 tttcggcaga agtggtgttt ttgttacaag agggccgctt tggctgggca ttactgaacg
     3721 ttttcgtcaa ccttctgggg tcttttgcca tgaccgcact ggcattctgg ctgttttcgg
     3781 cctcaaccgc acactaaaga ccaaaaaaaa cccgctgatt aagcgggttt tgaattcttg
     3841 ctgacgtatc ttacagagcg attacgtttg cagcagaagg gcctttggca ccgttagtga
     3901 tttcgaactc tacgcgctga ccttcagcaa gagttttaaa accattagtc tggattgcag
     3961 agaagtgtac gaacacgtct ttgctgccgt cttccggagt aatgaaaccg aatcctttgg
     4021 actcattaaa ccacttaacg ttacctttaa tcttagacat caaaattacc tttacatgaa
     4081 aaatagacac aaatgctgtg tcggttacca gtacaccaat tgtggtacgc tttgtccagt
     4141 gcaactttac taaaaagtga taaaaatcgc gttatttttg atgctactta ttttagttat
     4201 tgattttaaa gaagttacta aaacttcatt tgtctcaaaa ctgaaagcgc atccaggcaa
     4261 agtacacatt gccattgttg taggtacccg gaatgtaggt catctgaaaa gtaactgggc
     4321 cataacccac ggaggccaat ggcagtagaa ccgggagagg gatgtaattc cagttatcgc
     4381 gtgccgttac gccagcggtg aatcccagac ctaaatgaaa attttcatcc gccagcggtc
     4441 gccaggtact ttcccatccg tatccggcaa tcggttccca tttgttccac gagtccttaa
     4501 atgccatggc atacaggcca tgccagtttc ctttttcatc ccaacgcgac aggccaaaac
     4561 cgccacccca cggtcgctcg ttatagcgat cggttttttc tttgtcgtaa gcgaaacgtg
     4621 catgccaggt gatggcagga atatataaat cataatgttc aggctgttgc caggtttgta
     4681 caatattttc tctaaacgtt gtcatccact catctgcgtt agcaaaaact ttaccaacgc
     4741 tgattaactg aataaaaaca aaggaaaaga tagcgacata tttactcacg ttcatttgtg
     4801 accataaaac atttatcaaa aatctactac
//
LOCUS       DBFIFI010000270         4738 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.270, whole genome shotgun sequence.
ACCESSION   DBFIFI010000270 DBFIFI010000000
VERSION     DBFIFI010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4738
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.270"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..1267
                     /gene="garP"
                     /locus_tag="N7B39_004471"
     CDS             <1..1267
                     /gene="garP"
                     /locus_tag="N7B39_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HGU2424317.1"
                     /translation="FIVTAVNYADRATLSIAGTEVAKELQLSAVSMGYIFSAFGWAYL
                     LMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGFVDMFPLAWAGISMFFMRFMLGFS
                     EAPSFPANARIVAAWFPTKERGTASAIFNSAQYFSLALFSPLLGWLTFAWGWEHVFTV
                     MGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISENGAVVDMDHKKPGSAAASGPKLH
                     YIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIYLVQEKGMSILKVGLVASIPALCG
                     FAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLLASTIILCNYTNNTTLVVMLMALA
                     FFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGNVASIVTPLVIGYLVSELHSFNAA
                     LVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            1283..2053
                     /gene="garL"
                     /locus_tag="N7B39_004472"
     CDS             1283..2053
                     /gene="garL"
                     /locus_tag="N7B39_004472"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HGU2424318.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKTIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            2083..2973
                     /gene="garR"
                     /locus_tag="N7B39_004473"
     CDS             2083..2973
                     /gene="garR"
                     /locus_tag="N7B39_004473"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HGU2424319.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            3070..4215
                     /gene="garK"
                     /locus_tag="N7B39_004474"
     CDS             3070..4215
                     /gene="garK"
                     /locus_tag="N7B39_004474"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HGU2424320.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYAEVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            4248..4624
                     /gene="rnpB"
                     /locus_tag="N7B39_004475"
     ncRNA           4248..4624
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="N7B39_004475"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT         1088 a         1149 c         1377 g         1124 t
ORIGIN      
        1 ttttattgtt accgccgtta actacgccga tcgtgcaacg ctgtctattg ctggtaccga
       61 agtggcaaaa gagttgcagt taagtgcggt ttcgatgggt tacatctttt ctgcttttgg
      121 ctgggcctac ttgctgatgc aaatccccgg cggctggctg cttgataagt ttggctcgaa
      181 aaaagtttac acctacagcc tctttttctg gtcgctattc accttcctgc aaggctttgt
      241 tgatatgttc ccgctggcct gggcagggat ctccatgttc tttatgcgct ttatgctcgg
      301 cttctcggaa gcgccatcat tcccggcgaa cgcccgtatt gtcgccgcct ggttcccgac
      361 gaaagaacgt ggtactgcct ccgccatctt taactcggcg caatatttct cgctggcgct
      421 cttttcgcct ctgcttggct ggctgacttt cgcctggggc tgggagcacg tctttaccgt
      481 tatgggggtg attggttttg tgctgacggc gctgtggatc aaattgattc ataacccgac
      541 agatcaccca cgtatgtctg cggaagagct gaagtttatc tctgaaaatg gcgcggtggt
      601 cgatatggac cacaaaaagc cgggcagtgc ggcagcaagc ggacccaaac tgcattacat
      661 caagcaattg ctctctaacc gcatgatgtt gggcgtattt ttcggacaat attttatcaa
      721 caccatcacc tggttcttcc tcacctggtt cccgatttat ctggtgcagg aaaaaggtat
      781 gtcgattctg aaagtgggtc tggtcgcctc gattccagca ctgtgtggtt ttgcgggcgg
      841 cgtgctggga ggtgtcttct cggattatct gatcaaacgc ggtttatccc tgaccctggc
      901 acgtaagcta ccgattgtgc tgggaatgtt gctggcttcc accatcatct tatgtaacta
      961 caccaacaac accacgctgg tggtcatgct gatggcgctg gctttctttg gtaaaggatt
     1021 tggtgcgctg ggctggccgg tgatttctga caccgcgcct aaagagattg ttggcctctg
     1081 cggcggcgtc tttaacgtct ttggcaatgt tgcctccatt gtcactccac tggtgattgg
     1141 ctacctggta agtgaactgc actccttcaa tgcagcactg gttttcgtgg gatgttcagc
     1201 gctaatggcg atggtctgct acctcttcgt agttggcgac attaaacgta tggaattgca
     1261 gaaataagca aaggtataag cgatgaataa cgatgttttc ccgaataaat tcaaagccgc
     1321 actggctgcg aaacaggtac aaattggttg ctggtcagca ctctctaacc cgattagcac
     1381 tgaagttctt ggtttggctg ggtttgactg gctggtgctg gatggcgaac atgcgccaaa
     1441 cgatatttca acgtttattc cgcagttaat ggccctgaaa ggcagcgcca gcgcgccagt
     1501 agtgcgagtg ccgaccaacg agccggtaat tattaagcgt cttctggata tcggtttcta
     1561 taacttcctg attccttttg tagaaacaaa agaggaagca gagcaggcgg tggcatcgac
     1621 gcgttatccg ccagaaggca ttcgcggcgt ctccgtttct caccgcgcca atatgtttgg
     1681 caccgtggcg gattatttcg ctcagtcgaa caagaacatc actattctgg tccagataga
     1741 aagtcagcag ggcgtagata acgtcgatgc tattgccgct accgaaggcg tagacggcat
     1801 cttcgtcggc cccagcgatc tggccgcggc attaggccat ctcggcaatg catcacaccc
     1861 ggatgtacaa aaaacaattc agcacatttt taaccgtgcc agcgcgcacg gcaaacccag
     1921 cggtatcctc gcgccggtcg aagccgatgc gcgtcgttat ctggaatggg gcgcgacgtt
     1981 tgtggctgtc ggcagcgatc tcggcgtctt ccgctctgcc actcagaaac tggctgatac
     2041 ctttaaaaaa taaccaccac cgcaacaaga gagatgattg atatgactat gaaagttggt
     2101 tttattggcc tggggattat gggtaaacca atgagtaaaa accttctgaa agcaggttac
     2161 tcgctggtgg ttgctgaccg taacccagaa gctattgctg acgtgattgc tgcaggtgca
     2221 gaaacagctt ctacggctaa agcgatcgct gaacagtgcg acgtcatcat aaccatgctg
     2281 ccaaactccc ctcatgtgaa agaggtggcg ctgggtgaga atggcattat tgaaggcgcg
     2341 aagccaggta cggtattgat cgatatgagt tctatcgcac cgctggcaag ccgtgaaatc
     2401 agcgaagcgc tgaaagcgaa aggcattgat atgctggatg ctccggtgag cggcggtgaa
     2461 ccgaaagcca tcgacggtac gctgtcagtg atggtaggcg gcgacaaggc tattttcgac
     2521 aaatactatg atttgatgaa agcgatggcg ggttccgtgg tgcataccgg ggaaatcggt
     2581 gcaggtaacg tcaccaaact ggcaaatcag gtcattgtgg cgctgaatat tgccgcgatg
     2641 tcagaagcgt taacgctggc aactaaagcg ggcgttaacc cggacctggt ttatcaggca
     2701 attcgcggtg gactggcggg cagtaccgtg ctggatgcca aagcgccgat ggtgatggac
     2761 cgcaacttca agccgggctt ccgtattgat ctgcatatta aggatctggc gaatgcgctg
     2821 gatacttctc acggcgtcgg cgcacaactg ccgctcacag ctgcggttat ggagatgatg
     2881 caggcactgc gtgcagatgg tttaggaacg gcggatcata gcgccctggc gtgctactac
     2941 gaaaaactgg cgaaagtcga agttactcgt taatgacgtc gtgcccggat ggcgtattgc
     3001 aatccgggcc tggaatccag gccgaataag agaaacgccc tccggcgtca tgtggtaaca
     3061 ggcataggta tgaaaatcgt aatcgcccca gactcttata aagaaagttt atctgccagc
     3121 gaggttgcgc aggcgataga aaaaggattt cgggaaattt ttcctgatgc acagtacgtt
     3181 tctgttcctg ttgccgacgg tggcgaagga acggtggaag cgatgattgc agccacccag
     3241 ggttccgaac gtcacgcctg ggttacaggg ccgctgggcg agaaagtgaa tgccagttgg
     3301 gggatctccg gcgatggcaa aaccgcgttt attgaaatgg cggcggccag tgggctggag
     3361 ctggtacctg cggaaaaacg cgatccactc gtgaccactt cacgcggcac aggcgagtta
     3421 atcctgcagg cgctggagag cggcgcgaca aacattatta tcggcattgg cggcagcgct
     3481 acaaatgatg gcggcgcagg catggtacag gcgctggggg cgaaattatg cgacgccaac
     3541 ggcaatgaaa ttggttttgg cggcggtagt cttaatactc tgaatgatat tgatatttcc
     3601 ggcctcgatc cgcgcttaaa agattgcgtc attcgcgtcg cttgtgatgt caccaatccg
     3661 ctggtgggcg ataacggcgc atcgcgcatc tttggcccac aaaagggagc cagtgaagcg
     3721 atgattgttg agctggacaa taacctctct cactatgccg aggtcattaa aaaagcgctg
     3781 catgttgatg tgaaagatgt ccccggcgca ggagctgcgg gtggtatggg cgcggcgcta
     3841 atggcgtttc ttggtgcgga actgaaaagt ggtattgaaa tcgtcactac ggcgctgaat
     3901 ctggaggaac atattcacga ttgtacgctg gtgatcaccg gtgaagggcg tattgacagc
     3961 cagagtattc acggcaaggt accgattggt gtcgcaaacg tggcgaagaa gtaccataaa
     4021 ccggtgattg gcattgcggg tagcctgacc gatgatgttg gcgttgtaca tcagcatggc
     4081 attgatgcgg tcttcagcgt attgaccagc ataggtacgt tggacgaagc attccgcggg
     4141 gcttatgaca atatctgccg tgcttcacgt aatatcgccg cgacactggc gattggaatg
     4201 cgcaacgcgg ggtgacaagg gcgcgcaaac cctctatact gcgcgccgaa gctgaccaga
     4261 cagtcgccgc ttcgtcgtcg tcctcttcgg gggagacggg cggaggggag gaaagtccgg
     4321 gctccatagg gcagggtgcc aggtaacgcc tgggggggaa acccacgacc agtgcaacag
     4381 agagcaaacc gccgatggcc cgcgcaagcg ggatcaggta agggtgaaag ggtgcggtaa
     4441 gagcgcaccg cgcggctggt aacagtccgt ggcacggtaa actccacccg gagcaaggcc
     4501 aaataggggt tcataaggta cggcccgtac tgaacccggg taggctgctt gagccagtga
     4561 gcgattgctg gcctagatga atgactgtcc acgacagaac ccggcttatc ggtcagtttc
     4621 acctgattta cgtaaaaacc cgcttcggcg ggtttttgct tttggagggg cagaaagatg
     4681 aatgactgtc cacgacacta tacccaaaag aaagcggctt atcggtcagt ttcacctg
//
LOCUS       DBFIFI010000271         4725 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.271, whole genome shotgun sequence.
ACCESSION   DBFIFI010000271 DBFIFI010000000
VERSION     DBFIFI010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4725
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.271"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            87..629
                     /locus_tag="N7B39_004476"
     CDS             87..629
                     /locus_tag="N7B39_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HGU2424321.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            729..1931
                     /locus_tag="N7B39_004477"
     CDS             729..1931
                     /locus_tag="N7B39_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HGU2424322.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTLALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            2151..2933
                     /locus_tag="N7B39_004478"
     CDS             2151..2933
                     /locus_tag="N7B39_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HGU2424323.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCNAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTKLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPATVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYADVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            2948..4153
                     /gene="rhmD"
                     /locus_tag="N7B39_004479"
                     /pseudo
     CDS             2948..4153
                     /gene="rhmD"
                     /locus_tag="N7B39_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050032 - L-rhamnonate dehydratase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
     gene            4210..>4725
                     /locus_tag="N7B39_004480"
     CDS             4210..>4725
                     /locus_tag="N7B39_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2424324.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGH"
BASE COUNT         1079 a         1164 c         1296 g         1186 t
ORIGIN      
        1 tttttcgctc attgaatcct ctgcgccagg cagtacaatc ccgcagcttt gggaattttg
       61 attaattgct tgtgggaaag gggataatga aaaaaattgc gctagcaggt ctggccggaa
      121 tgctgttggt ttctgcatcg gtcaatgcaa tgagcatcag cggccaggcg ggtaaagaat
      181 acaccaatat tggtgtcggt tttgggactg aatcgacggg cctggcttta agcggtaact
      241 ggacacataa cgacgacgac ggtgacgtcg cgggcgtggg gctggggttg aatctgcctc
      301 tcgggccgtt aatggcgacc gttggcggaa aaggcgttta caccaacccg aattacggcg
      361 atgaaggcta tgccgcagcg gtaggaggtg gtttgcagtg gaaaattggc aacagcttcc
      421 gtttgtttgg cgagtattac tactctccgg attcactctc cagcggtatt aaaagttatg
      481 aggaagcgaa tgctggcgcg cgttacacca ttatgcgtcc agtcagtatt gaggcgggtt
      541 atcgctacct gaatctgtcg ggtaaagacg gtaaccgcga caacgccgtg gctgacggcc
      601 cgtatgttgg ggttaacgcc agtttctgat cccttccggc gcggcattct gtcgcgccat
      661 tcagtttctt actacgcctt atctcccttt gcgttatagt gattcgataa caaaagcagg
      721 agataaccat gttaaaagtg gaaatgctat ccaccgggga tgaagtgtta cacgggcaaa
      781 tcgttgacac taacgctgcc tggctggccg attttttctt tcatcagggg ttgccattat
      841 ctcgccgcaa tacggtgggg gataaccttg atgacttagt caccattctt cgcgaacgta
      901 gtcagcacgc cgatgtgctg atcgttaacg gcgggctggg accgaccagc gatgatttaa
      961 gcgcactcgc cgctgcgaca gcaaaaggtg aaggcctggt gctgcatgaa gcctggctca
     1021 aagagatgga acgctatttc cacgaacgtg gacgagtaat ggcaccgagc aaccgtaaac
     1081 aagcggagct gcctgccagt gctgaattta tcaataaccc ggtaggcacc gcctgtggtt
     1141 ttgccgtgca gcttaatcgt tgcctgatgt tctttactcc cggcgtaccg tcagaattta
     1201 aggtgatggt cgagcacgaa atcctgccgc gtctgcgcga gcgtttttct ttaccacagc
     1261 cgccggtttg tctgcgttta actacctttg gtcgttcgga aagcgatctg gcacaaagcc
     1321 tggacactct acaactgccg ccgggcgtaa caatgggcta tcgctcctca atgccgatca
     1381 tcgaactgaa actcaccgga ccggcaagcg agcaacaggc gatggaaaaa ctgtggctgg
     1441 acgttaaacg tgttgccgga cagagcgtga ttttcgaagg caccgaagga ctgcccgcgc
     1501 agatcagtcg cgaattgcaa aaccgccagt tcagcctgac gttgagcgag caattcaccg
     1561 gtggtttatt ggctttgcaa ctttctcgcg caggtgctcc actgctggcg tgtgaagtgg
     1621 ttccttcaca ggaagaaacc ctggcgcaaa ctgcgcactg gattacagaa cggcgagcca
     1681 accattttgc cgggctggca ctggctgttt cgggtttcga gaacgagcat ctcaactttg
     1741 cgctagccac gccagatggc actttagctc tgcgtgtgcg tttcagcact acacgctata
     1801 gcctggctat ccgtcaggaa gtgtgcgcaa tgatggcact gaatatgcta cgccgttggt
     1861 taaacggcca ggacattgcc agtgagcatg gctggattga ggttgttgag tccatgacct
     1921 tatccgtctg attccgccac tctgtgccgg tttactgcac cggcacattc cccctccggc
     1981 aaaacttgtc caacctgttg tactcaataa gaaaaaatcc gtgcgctgat tcacaaattt
     2041 gtgcgtaatt tctccagtcc gttggacgag gagcgggatt tgatgcgctt cacaatttta
     2101 ctccccctga gcaacgagac tgattccagt atctaagact ggagtccagt atgttggaat
     2161 caagcaaagt tcccgccctg acacgggcta ttgatattct caatttgatt gcccgcattg
     2221 gcccctgtaa tgctgcgacg atcattgaca cattggggat ccctaaaagc acggcctatt
     2281 tgctgcttaa tgaactcagg cgtcagcgtt ttcttagcct tgatcaccag gaaaattttt
     2341 gtttgtggac caagctggtg gagctttccg gtcatgcgtt aagcaagatg gatctccggg
     2401 aactggctcg accgcgtttg acgcaactga tggataccac cgggctgtta tgtcacctgg
     2461 ggattattga taacggcagc gcttattaca ttttgaaagt ggagtcatca gccactatca
     2521 gcgtgcgttc acatgaagga aaaagccttt cgctttatcg ctccggaata ggtaaatgcc
     2581 tgcttgcctg gcaacctgca actgtacagc aaagtattat cgaagggctg gtctgggagc
     2641 aggcgacacc cacaacgatt actcatccac agcaacttca cgaagaactg gcgcgtattc
     2701 gccgtcaggg ctggagctat gacaacggcg aagattacgc cgatgtccgt tgtgtcgctg
     2761 cgccggtgtt taacgccaat aacgagctta ccgctgcgat ctcggtggtc ggtacccgct
     2821 tacaaatcaa tgaagaatac cgtgattatc tggcgggcaa ggccattgcc tgcgccaggg
     2881 atatttcacg tctgttggga tggaaaagtc cctttgactt acaagcctca taacaatgga
     2941 gaacatcatg accctaccaa aaattaaaca ggttcgcgcc tggtttactg gcggtgcgac
     3001 agcagaaaaa ggcgctggcg gcggtgatta tcacgaccag ggggcgaatc actggataga
     3061 cgatcatatt gccaccccga tgagtaaata ccgcgattac gagcagttac gccagtcatt
     3121 tggcattaac gttcttggca cgttggttgt tgaagtcgaa gcagaaaacg gccagaccgg
     3181 attcgctgtt tcgacagccg gtgaaatggg ctgttttatc gtcgaaaaac accttaaccg
     3241 tttcattgag ggtaaatgtg tcagtgatat caaactgatc catgatcaaa tgctcaatgc
     3301 caccctgtat tactccggct ctggtggcct ggtgatgaac acgatttcct gtgtcgatct
     3361 ggctctgtgg gatctgttcg gcaaagtggt cgggcttccg gtttataaac ttttaggcgg
     3421 cgctgttcgt gatgagattc agttctacgc cacaggtgcg cgtccggatc tggcaaaaga
     3481 gatgggcttt atcggtggca aaatgccgac gcactggggg ccacatgatg gcgatgcggg
     3541 gatccgcaaa gatgccgcta tggtcgcgga tatgcgtgaa aaatgcggtg aggatttctg
     3601 gttaatgctc gactgctgga tgagtcagga cgtgaactat gcgaccaaac tggcccacgc
     3661 ttgcgcgccc tataacctga aatggatcga agagtgcctg ccgccacagc agtatgaagg
     3721 ttatcgcgaa ctgaaacgca acgcgccagt cggaatgatg gtcaccagcg gtgagcacca
     3781 cggcacactg caatcttttc gtacgctttc agaaaccggt atcgacatta tgcagccgga
     3841 tgttggctgg tgcggtggct taaccacgct ggtggaaatt gccgcaatcg ccaaatccag
     3901 ggggtaactg gtggtgccgc acggttcgtc tgtttactcc caccatgcgg tgatcacctt
     3961 cactaatacg ccattcagcg aattcctgat gactagtccg gattgttcaa cgatgcgtcc
     4021 gcagtttgac ccgattctgc tcaatgagcc ggttccggtg aatggtcgta ttcataaatc
     4081 agtgcttgat aaacccggtt ttggcgtcga actcaatcgt gactgcaatt tgaaacgccc
     4141 ctacagccac taatcactcc gggcgttgct gtgacaacgg taacgcccac atattctaag
     4201 gagcttttta tgagcaccgc tttgcttgac gccgtggtga agaaaaaccg tgtgcgttta
     4261 attccgttta tgttggcgct gtatgtgctg gcgtttctcg accgttcgaa tatcggtttt
     4321 gccaaacaga cctaccagat tgataccggg ttgagtaatg aagcttatgc gctgggagca
     4381 ggcattttct ttgtggtata tgcgtttctg ggtgttccgg cgaatctttt gatgcgcaaa
     4441 ctgggggcca gaacctggat tggtacgaca acactgctgt ggggatttct ttcggcagcc
     4501 atggcatggg ccgatactga agcgaaattt ctgatagttc gcactctgct tggtgctgcg
     4561 gaggccggat ttttccctgg tatgatttat ctcacctcgc aatggtttcc gcagcgtaat
     4621 cgcgccagca ttatggggct gttctatatg ggcgcaccgc tggcgttaac actgggatca
     4681 ccgctttccg gcgcgctgct ggagatgcat ggatttatgg ggcat
//
LOCUS       DBFIFI010000272         4719 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.272, whole genome shotgun sequence.
ACCESSION   DBFIFI010000272 DBFIFI010000000
VERSION     DBFIFI010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4719
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.272"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            150..293
                     /locus_tag="N7B39_004481"
     CDS             150..293
                     /locus_tag="N7B39_004481"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424325.1"
                     /translation="MNNFSKTSTMQIKRIKPSIIYRLLLIVLGIPMVIYGLVCPLTIE
                     TRD"
     gene            complement(512..2050)
                     /gene="aldB"
                     /locus_tag="N7B39_004482"
     CDS             complement(512..2050)
                     /gene="aldB"
                     /locus_tag="N7B39_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HGU2424326.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKNRYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAEAWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(2158..3453)
                     /locus_tag="N7B39_004483"
     CDS             complement(2158..3453)
                     /locus_tag="N7B39_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HGU2424327.1"
                     /translation="MAIKSPPTLIPLSHLSGEELQAHLRFNRVTDEKGRYLPFDELQY
                     RIKKGENVDVAWTLTRLARNAAIQRINYCNEAGEQAGFNITPVIAEACELVDKRATAL
                     ALKDQTERLRGAGAELSQLRLEEPITSSQLEGANTTTLVARKMLETGRSPRTEDEHMI
                     AGNARLMAEIPHLLAEPLTPALIRQLHAIGMGGINDAKYRPGEFRETDDVVIADYDGN
                     IVHQPPAAALLPERLEKVCQWLNSHEGYIHPLVRACILHFMLAHEHPFRDGNGRTSRA
                     LFYWYMLKSGYDVFKYISISRLLHAAPVKYAASYQYTESDGMDLTYFLEYQAGVIKRA
                     LQNWQQHIDEITQRSAKLDSVLFSSGVLKRLNPRQVTLLNVMLANPGKEYTVAEISAS
                     LGVSDNTVRADLRTIVKEGFAQEKKINDQQAVYFAHYPL"
     gene            complement(3583..>4719)
                     /gene="yiaY"
                     /locus_tag="N7B39_004484"
     CDS             complement(3583..>4719)
                     /gene="yiaY"
                     /locus_tag="N7B39_004484"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HGU2424328.1"
                     /translation="FFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTKLGMAG
                     DVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHDCAKGI
                     ALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIKMAIVD
                     KHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALKAVTMI
                     AENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLPHGVCN
                     AVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKVDIPAG
                     LRDLNVKEEDFAVLAANALKDACGFTNPIQATHEEIVAIYRAAM"
BASE COUNT         1133 a         1253 c         1141 g         1192 t
ORIGIN      
        1 acgcatatta tgaaatgcca tgatgcaatg caatttcctt atttatataa cgacatattg
       61 ttttttatat actttctgaa gtgtgctgcg tggcttttta acaggtcgag gcttatcata
      121 ttgaaataat ggaactatca gccaaactaa tgaataactt cagtaaaaca tccacaatgc
      181 agattaagcg tattaaacct tcaataatct accgtttatt gctgattgtc ctcggaatac
      241 caatggtgat ttacggtctg gtttgcccgc tcaccatcga aacgcgagat taaactcctg
      301 gcaaaacacg ccccagaaac gcacttttgt tggcgtagtc tgggttattg cacacgtagg
      361 tttaaaacgt aaattgtaac aacgtgcgct ttgtttatgc cggatgcggc gtgtacgcct
      421 tatccggcct acggtcaggt ccccataggc atgataagac gcgtaagcgt cgcatcaggc
      481 aattaatacc cgatgcgacc agcttcttac atcagaacag ccccaacggt ttatctgagt
      541 agctcactag caggcacttg gtttgctggt aatgctccag catcatcttg tgggtttcgc
      601 gaccgatacc tgattgtttg tagccaccaa acgccgcatg tgccgggtaa gcatgataac
      661 agttggtcca cacgcgccca gcctgtatgc cgcgccccat cttataggcc agattaccgt
      721 tgcggctcca gacgcccgcg cccaggccat attgcgtatc gttcgccagc tccagcgctt
      781 cttccatcgt tttgaaggta gtcaccgcca gcaccgggcc aaaaatctcc tcctggaaca
      841 cgcgcatatt gttctgacca aacagaatcg tcggttcgag gtagtagccg tctttcagtt
      901 caccttccag cagcttgcgc cgcccgccgg tgagcacatc agcgccctct tttttaccga
      961 tatcaatgta gttgaggatg gtttccagtt gcccgtgaga aacctgcgca cccatttgcg
     1021 tcacgctgtc gagtgggtta ccgctgcgaa tgctttcgac acggcggatg gcgcgttcca
     1081 taaagcgttc gtagattgac tcctgcacta aggcacgact cggacaggtg caaacttcgc
     1141 cctggttaaa ggcaaacagt gcaaagcctt ccagcgcttt atcgaaaaag gcatcttctt
     1201 catccatcac atcagcaaag aagatatttg gcgacttacc gcccagctcc agcgtcaccg
     1261 gaataatgtt ttgcgtggcg tattgcataa tttgttgacc cacttccgtt gagccggtaa
     1321 acgccacttt ggcgatgcgt ttcgaggtcg ccagatattc gccaatttcc ccacctgcac
     1381 cgttgaccac gttcaccacg cccggcggca gtaaatcacc gacgatttcc attagcagca
     1441 gtacagaaag cggggtaaga cgtgcgggtt tcagcaccac acagttgccc gccgccagcg
     1501 cgggagccat tttccagctc gccatcagca gcgggaagtt ccacgggata atctgcccca
     1561 ccacgcctaa cggttcatgg aaatgatagg ccacggtttc gctatcaact tcactgatcc
     1621 caccttcctg cgcccgaata cacgaggcga aatagcggaa atggtcaatc gccagcggta
     1681 catccgcagc actggtttcg cgaatgggtt tgccgttatc ccaggcttca gctgtcgcta
     1741 acagctcgag gttttgttcc attcgatcgg caatcttaaa cagaatcgcc gcgcgatcct
     1801 gcaccgaggt gtgcgcccat ttatctttca ctttgtgcgc agcatccagc gccagatcga
     1861 tgtctcgttt gcccgaagac gccacttcgc acagcagctg cccggtcacc ggcgtcaggt
     1921 tctggtaata ctcaccatcg gcaggggcta cccattcgcc gccaataaag ttgtcatagc
     1981 ggttttttaa cttgaggggg aaaccatact cgccgggctt aatctgtgct gaagggggat
     2041 tattggtcat gatcgtctcc ttgcggtgtg aggtataaca agggtagacg tggctggcga
     2101 aatcttcgcc agtcggtaac agcttttcga cggctatcac gaatttacgg gcaagggtta
     2161 cagcggataa tgcgcgaagt acacggcttg ctggtcatta attttcttct cttgcgcaaa
     2221 cccttcttta acaattgtcc gtaaatcagc tcgaaccgta ttatcagaga cgcccaggct
     2281 cgcgctaatt tcagcaactg tgtactcttt ccccggattg gcgagcatca cattcaggag
     2341 cgttacctgg cgcggattca ggcgtttaag cacaccagag ctaaacagca cactatcgag
     2401 ttttgcgctt cgttgtgtaa tttcatcgat atgctgctgc cagttttgca gagcacgttt
     2461 aatgacaccc gcctgatatt caaggaaata agtcagatcc ataccatctg attcagtgta
     2521 ctggtagctg gcggcatatt tgaccggtgc agcatgaagc aaacggctga tggaaatata
     2581 tttaaagaca tcgtagcccg atttcagcat ataccagtaa aacagcgccc gactggtgcg
     2641 cccgttgcca tcccgaaacg gatgttcatg ggccagcata aaatgcagaa tgcaggcacg
     2701 gaccaacgga tgaatatacc cttcatggct gtttaaccac tggcaaactt tttccagccg
     2761 ttccggtaac aatgccgcag cgggcggttg atgcactata ttgccatcat agtcggcgat
     2821 cacaacatca tctgtttcac gaaattctcc cggacgatat ttcgcgtcat tgattccacc
     2881 cataccaatg gcatgtaact ggcgaataag cgcaggtgtc agcggttctg cgagtaagtg
     2941 cggaatttcc gccatcagcc gggcatttcc cgcaatcata tgctcgtctt cagtgcgtgg
     3001 cgaccgtcct gtttccagca tttttctcgc caccagcgtt gtagtattcg caccttccag
     3061 ctgggagctg gtgatcggct cttccaggcg taactggctt aactcggctc cggccccgcg
     3121 taaccgttca gtctgatctt tcagcgccag cgcggtagca cgtttgtcga caagctcgca
     3181 cgcttctgca atcaccgggg taatgttgaa tccggcctgt tcacccgctt cattgcaata
     3241 attaattcgt tggatcgccg cgttgcgcgc cagacgggta agcgtccacg ccacatcgac
     3301 gttttctcct tttttgatgc ggtactgcaa ctcatcaaaa ggcagataac gtcctttttc
     3361 atcggtaacc cggttaaagc gcaggtgggc ctggagttcc tcacccgata agtgtgataa
     3421 aggaatcagc gtgggaggtg atttgatagc catttgctta tctcaaaaaa gcgcaatatt
     3481 gagatagtgt atagcaggaa agagaaacta tctcaaaaaa agcaagtttt gagatagttg
     3541 aaaaaatggc tgacaatttg ccagccgttg tggaaatgat gattacatcg ctgcgcgata
     3601 aatcgccaca atttcttcgt gagttgcctg gatcgggtta gtaaagccac aggcatcttt
     3661 cagggcatta gccgccagaa ccgcgaaatc ttcttctttc acgttcaggt cgcgtaggcc
     3721 tgccgggata tccactttct tcgccagttc acggatggcg ttaatgcagg cttcagcacc
     3781 ttccgcgtcg tttttacctg tcacgttcac gcccattgca gcggcacagt cacgcagacg
     3841 tgcagcggcg actttgctgt tgaatacctg aacatgcggc agcagaacgg cgttacatac
     3901 accgtgtggc aggttgtaga aaccgcccag ctggtgcgcc atcgcatgaa cataacccag
     3961 agaagcatta ttgaacgcca taccggcgag gaactgggca taagccattg cttcacgcgc
     4021 tttcgcatta ctgccatctt caacggctaa cggcaggttt tcggcaatca tggtcacggc
     4081 tttcagtgca caagcgtcag tgatcggcgt ggcggcaata gaaacatacg cttcgatagc
     4141 gtgcgttaag gcatccatac ccgttgcggc ggtcagtgac ttcggcatac caatcatcag
     4201 agaggagtca ttgacagaaa gcagcggagt gacatgttta tcaacaatcg ccattttgat
     4261 atgacgcgct tcgtcagtga tgatgcagaa acgggtcatt tcagaggccg tacccgccgt
     4321 ggtattgatg gcgatcatcg gcagctgcgg ttttgcagag cggtcaacgc cttcgtaatc
     4381 gcgaatatcg ccgccattgg ctgccaccag cgcaatacct tttgcgcagt cgtgtggaga
     4441 accaccgcct aaggagatca cgctatcgca attattctct ttaagtaatt tcaaacctgc
     4501 ggcgacgttt tccgtggtgg ggttaggttg ggtgccatca taaataacgc taaaaatatt
     4561 gcgttcttcc agtgcttttt gcacatcgcc cgccatacct aatttcgtta acatactgtc
     4621 agtgacaatt aaggtacggg taaatccata atctgccatc atattcattg catcagtcaa
     4681 tgaatcagcg ccgatgacat tcacagaagg aataaagaa
//
LOCUS       DBFIFI010000273         4693 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.273, whole genome shotgun sequence.
ACCESSION   DBFIFI010000273 DBFIFI010000000
VERSION     DBFIFI010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4693
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.273"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(147..1466)
                     /gene="digH"
                     /locus_tag="N7B39_004485"
     CDS             complement(147..1466)
                     /gene="digH"
                     /locus_tag="N7B39_004485"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HGU2424329.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            complement(1597..3132)
                     /gene="gadC"
                     /locus_tag="N7B39_004486"
     CDS             complement(1597..3132)
                     /gene="gadC"
                     /locus_tag="N7B39_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HGU2424330.1"
                     /translation="MATLVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            complement(3288..4688)
                     /gene="gadB"
                     /locus_tag="N7B39_004487"
     CDS             complement(3288..4688)
                     /gene="gadB"
                     /locus_tag="N7B39_004487"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HGU2424331.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTL
                     YDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSD
                     HPKLQGIAQQNSFKHT"
BASE COUNT         1199 a         1179 c         1188 g         1127 t
ORIGIN      
        1 attaggggat ttatggctgt ttaactaagc gtggttaatt gcgtggttaa tttgacttta
       61 gtaagcatga ttgctggtgg gatagttcaa gagggtaaca agccagtggg taaagcaccg
      121 gcttgttaca aagtaagaat gggagtttaa ctgccccaac gactttgcag atagctgacc
      181 gcttgttgag tctgcggttt attcagatag tcctcacgga acaagatggt gccgctaatt
      241 tctggtaccg catcgttaag atcgagctgc tttttcagct ccggtacgcc gccgttaatc
      301 atccagtctg gctctatctt tgaaggttca cccactttat agaaggcgat accgatatac
      361 aggcgggtcc tggtcggttt aacgacatcc gcccaccatt ttgccaacac gtcataacgc
      421 gcggcactcc gtgagaacgg ccagtaaatt tggggagcaa tgtaatccag caatccttgt
      481 tccacccatc gacgagtgtc agcgtaggat tcgtcatagg ctgccgcgcc tcgggtatcg
      541 gaaccgagcg gatcgtgtga tcggttacgc cacacgcctg ccgggctaac accaaattcg
      601 actcccggct taatgctttt aatggtgtgc gatacctttg caattaactg ctgagtattg
      661 ttgcgccgcc agtctgcttt tgacgcaaat gcgcctccgt atttacggta cgtttcgtta
      721 tcatttagcc gtgaacccgg tgactcagta tagaaatagt cgtcaaactg cacgccatct
      781 accggatagc gggaaaccac ttctgcgact attgatgtga tccagtcctg aacctcaggg
      841 atgcccgggt cgaggacaaa gcgatcgcca gacgttctga tccagtcacg gtgttgcaca
      901 tagacgctcg ccggttgttg agacagagtg ctattcagtt ccctgatagt accgggcttc
      961 gtattaaccg atacgcgata ggggttaaac caggcgtgta ctttcatccc acgcttgtgg
     1021 gcttcgtcga gcataaattg cagcggatcg taacccggat tttcaccaat cttaccggtc
     1081 ataagatcgg accacggcaa aattttcgat ggccacaggg cggtaccgtc cggcttgacc
     1141 tggaaaaaga ccgtgtttat accgagacgt tgcaaatgat ccagtttgtc gatcatcgcc
     1201 tgttgttgta cacgggcccg gctggtgggg ttactaatgt taaccgagga aaccggtggc
     1261 cagtcgaggc gagacaccgt ggccagccag atgccacgca tcggttgtga cgattgttgc
     1321 gtcgtggctg gtggctttga acccgctggt ggtgtcacca tggactctgg aggcgtgctt
     1381 ttacaactac acagtaaaag tgccagtgca accagtatcg ctggtcttct aatcgttaat
     1441 ttcttgtttc gggagcagat atccatatgt gctagtttcc ggtaaacaga tgtgcgctca
     1501 ttctcgtact tatcccggtt aagtcaatac gacagcaagc acgaaaaagg gagcgatgaa
     1561 ttatcgctcc cttatcttat aaccattcag acatagttag tgtttcttgt cattcatcac
     1621 aatatagtgt ggtgaacgtg cacgcgggtg caggaagaag tgaccttttg gtgcgttctg
     1681 actgttgatt ggctccagag tgacgccggt attcgctttg cctttacgat catgaacagc
     1741 atagagaata aagggcaggg caagtaccac caggaaacta acgaccagta attcaacata
     1801 catatcggta gagtcaccct ggatgttatc cggcggcagg aaggaaacaa taaacgccat
     1861 aattgaagtc agcagaccga caattgccac gaccagtttc acccctttac caccagggat
     1921 attaaatgtg cgttttaagt caggatgttt aagaaccaac acaatgtagc caataaacag
     1981 catgaaataa gcacacagat aaatcaccac cgtcagcgcc agtgcgatca ggaaggacat
     2041 gttgttaccg ccaccggtat tggtgaggat gatcaacgcg atagaagtaa tcaccagctg
     2101 cgaaatgacc agcgttaccg gtacgccatt tttgttcatt ttagcgaatg ccgctggcag
     2161 caggtttttc tgcgctgtga catacatccc gcgagaagga ccaacaatcc aggaggcgat
     2221 ttccgccaga acacccagca acagcagtgc ggagatcacg cgaaccgtcc actcaatttc
     2281 cggtgccaca tgggacatca gaacggtaaa ggtttgcatt acccctgcgg agaggttgat
     2341 ttcattaccc ggaatgacca tcgcaataga caaaccgcca acagagctta agcagattgc
     2401 cgccaccatc agcagtaaca tagccagtgg atagtcgcgc cctgggttac tcatttcatt
     2461 gacgtgggtt gcggaagctt ctacgcccat ataactcaaa atgaaggcaa caaatacaac
     2521 cagggtgccc actttagaga agtcagggaa gaaggtcttc gaatccattt cgatagcaac
     2581 gggggcaccg gagtgcagat aaatagccgc taatgcaatc aaaataaatg caggtaacag
     2641 gataccggcg aagaagccaa ctttagcaat tcgcgccgtg tatttcgtgc caccaaactg
     2701 cgttaatgcc agcgcccaaa gaatgatgag tgctgcaata gttttggtaa tggggtcttc
     2761 attcagcgct ggccatttca ggatgtagga gagtgcccct aacacgaaat agagcatcgg
     2821 aataaaacca atggcgattt gcagatagcc aaatgagatc gctgcaaatc cccatctcgg
     2881 ccccagagta tttgataccc aggcgaagac accaccttct tcccagccgt cgacggtggc
     2941 catttccgca gcacaaagtc ccacgggaat aaaccataaa atcccgccta atagcaggaa
     3001 gaagactaat gaaaagcccg atgttgcgaa ggtagggtat tcataaacag ccattaccat
     3061 cgatgccgtt atggcaaaaa agccgagtaa tgtgagctgc ttagctttac ctgtctgtac
     3121 taatgtagcc atattatccc cctaaaacgg tattcctgtc ggaaccgcac ctgttttgtt
     3181 ctgaagcgta ttcagaacaa tattttccgt tgctaatgcc agtgaacaga ctttggaaat
     3241 tgtcccgaaa cgggttcgtt tcgggacacc gttaccgtta aacgttatca ggtatgttta
     3301 aagctgttct gttgggcaat accctgcagt ttcgggtgat cactgagata tttcagggag
     3361 gctttgtagt cttccagcaa cagttcagca aagtccattt cgaagccgcg acgacacata
     3421 atgcgcatca ccacgatgtc ggtggcttca ccgccgagag tgaaggccgg aacctgccag
     3481 ccgcgcagac gcagacgttc agagaggtca tacagggtgt atcccggatc ttcaccatct
     3541 ttcagtttga agcaaaccgc cggaatgcct tcgtccgggc gaccggtaca gatgaactca
     3601 tacggcccca gtttggcgat ttcatccgcc agataagcgg caacctggta agaggcgttc
     3661 tgtactttgg tatagccttc acgaccgagg cgcaggaatt catagtactg tgcaattacc
     3721 tgacccgccg ggcgggagaa gttgatggcg aaagtaccaa tctgaccacc gaggtagtca
     3781 acgttgaaca ccagttcctg cggcagcgct tcttcatcac gccagataac ccagccgcag
     3841 cccagcggag ccagaccgaa tttgtggcct gaagcactga tcgatttcac acgcggcagg
     3901 cggaagtccc agacgatatc cggggcgacg aacggtgcca ggaagccacc actggcggcg
     3961 tcgatgtgca tgtcgatgtc gataccggtg tcggcctgga atttatccag cgcatcgtgc
     4021 agcggttgcg ggaactcata gttaccggtg taggtcacgc cgaaagtcgg caccacgccg
     4081 atggtatttt cgtcgcaggc ttcaatcatg cgtttcggat ccataaacaa ctgaccgggg
     4141 cgcataggga tctcacgcag ctccacatcc cagtagcggg cgaatttatg ccagcagatt
     4201 tgcaccgggc cgcacaccag gtttggttta tccgttggtt tgcctgcagc ttccatacgc
     4261 ttgcgccaac gccatttcat cgccatcccg ccgagcatac aggcctcgga agaaccaatg
     4321 gtgttggtgc caacggcctg accatttttc ggcgcaggcg catgccacag atcggcaacc
     4381 atatttacgc aacgcaggtc gatggctgca gattgcggat attcttcttt gtcgatccag
     4441 tttttgttaa tggataaatc catcaacttg tggacgtttt cgtcgtccca ggtctggcag
     4501 aaagtggcca ggttctgacg agcgttgcca tcaagatata attcgtcatt gataatctga
     4561 aatgcgacat cgtcgcgcat ttcgtgcagc ggaaaacgtt ttgattctgc gatagtggaa
     4621 atagacttcg caccaaaacg tgaatcgagt agttccgacc ttaaatccgt tacttgcttc
     4681 ttatccattt taa
//
LOCUS       DBFIFI010000274         4625 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.274, whole genome shotgun sequence.
ACCESSION   DBFIFI010000274 DBFIFI010000000
VERSION     DBFIFI010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4625
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.274"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..177
                     /gene="kil"
                     /locus_tag="N7B39_004488"
     CDS             <1..177
                     /gene="kil"
                     /locus_tag="N7B39_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707273.1"
                     /note="Rac prophage Kil protein; Kil overexpression leads
                     to morphological defects and inhibits cell division;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HGU2424332.1"
                     /translation="VTPGDYVLHEGRTYIASANNIKKRKLYIRSLTTKTCITDCMIKV
                     FLGRDGLPVKAESW"
     gene            177..347
                     /gene="ydaE"
                     /locus_tag="N7B39_004489"
     CDS             177..347
                     /gene="ydaE"
                     /locus_tag="N7B39_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaE family protein"
                     /protein_id="HGU2424333.1"
                     /translation="MTKKIKCAYHLCKKDVEESKAIERMLHFMHGTLSKDEPRKYCSE
                     ACAEKDQMAHEL"
     gene            422..697
                     /locus_tag="N7B39_004490"
     CDS             422..697
                     /locus_tag="N7B39_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424334.1"
                     /translation="MITNYETTVVTTDDIVHEVNLEGKRIGYVIKTKNKETPFTVVDI
                     DGPSGNVKTLDEGVTKMCLVHIGKNLPAEKKAGFLATLIAMKLNGEI"
     gene            799..3471
                     /gene="recE"
                     /locus_tag="N7B39_004491"
     CDS             799..3471
                     /gene="recE"
                     /locus_tag="N7B39_004491"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HGU2424335.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWASDDFESTCATLDYLIVKSG
                     KKLSNYFKAVATNFPVVNDLPPEGEIDFTWSERYQLSKDSMTWELKPGAAPDDVHHQD
                     NAQETKELAGGLEENAQADAHGDCQDCEVSVATLRFTQRLLHIFTYAAGDRKYLHHAT
                     RKQREHITALEMDQENSYVQNLLLAIRGMAEPTTLDNAALLRLTDAIKAVFSITKKHQ
                     PYEFKNFISAWLDTEHIDRGLLTKEWRKGNRVSRITRTDSGANAGGGNLTDRGEGFVH
                     DQASLARDVATGILARSMDVDIYNLHPAHAKRIEEIIAENKPPFSVFRDKFITMPGGL
                     DYSRAIVVASVKEAPIGIEVIPAHVTEHLNKVLTETDHANPDPEIVDIACGRSSAPMP
                     QRVTEEEKQDDEEKPQPYCAMADEQATAETVEPDATEHHQDTQPLDAQSQVNSVDEKY
                     QKLRAELHETRKNIPPKNPVDADKLLAASRGEFVEGISDPNDPKWVKGIQTRDSVYQN
                     QPETEQNTPETVKTSPDVKQPEPVVQQKPEIVCNACGQTGGDNCPDCGAVMGDATYQE
                     TFNEENLDESQEKEPEEMEGPEHPHNENAGSDLHRDCSDETGEVADPVIAGDIEPGIY
                     YGISNENYHAGPGVSKSQLDDIADTPALYLWRKNAPVDTTKTKTLDLGTAFHCRVLEP
                     EEFSNRFIVAPEFNRRTNAGKEEEKAFLMECASTGKTVITAEEGRKIELMYQSVMALP
                     LGQWLVESAGHAESSIYWEDPETGILCRCRPDKIIPEFHWIMDVKTTADIQRFKTAYY
                     DYRYHVQDAFYSDGYEAQFGVQPTFVFLVASTTIECGRYPVEIFMMGEEAKLAGQLEY
                     HRNLRTLADCLNTNEWPAIKTLSLPRWAKEYAND"
     gene            3464..4273
                     /gene="recT"
                     /locus_tag="N7B39_004492"
     CDS             3464..4273
                     /gene="recT"
                     /locus_tag="N7B39_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecT"
                     /protein_id="HGU2424336.1"
                     /translation="MTKQPPIAKADLQKTQGNRAPAAIKNNDMISFINQPSMKEQLAA
                     ALPRHMTAERMIRIATTEIRKVPALGNCDTMSFVSAIVQCSQLGLEPGSALGHAYLLP
                     FGNKNEKSGKKNVQLIIGYRGMIDLARRSGQIASLSARVVREGDEFNFEFGLDEKLIH
                     RPGENEDAPVTHVYAVARLKDGGTQFEVMTRKQIELVRSQSKAGNNGPWVTHWEEMAK
                     KTAIRRLFKYLPVSIEIQRAVSMDEKEPLTIDPADSSVLTGEYSVIDNSEE"
     gene            4330..4524
                     /gene="ralR"
                     /locus_tag="N7B39_004493"
     CDS             4330..4524
                     /gene="ralR"
                     /locus_tag="N7B39_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system
                     endodeoxyribonuclease toxin RalR"
                     /protein_id="HGU2424337.1"
                     /translation="MRYDDVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEK
                     EALERWNKRTTGNNNGGVHV"
     gene            4517..>4625
                     /gene="rcbA"
                     /locus_tag="N7B39_004494"
     CDS             4517..>4625
                     /gene="rcbA"
                     /locus_tag="N7B39_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-strand break reduction protein RcbA"
                     /protein_id="HGU2424338.1"
                     /translation="MYKITATIEKEGGTPTNWTRYSKSKLTKSECEKMLS"
BASE COUNT         1441 a         1054 c         1100 g         1030 t
ORIGIN      
        1 gtgactcctg gcgattatgt tcttcatgaa ggccggacat atatcgcctc ggcaaacaat
       61 attaaaaagc gaaaacttta tattcgtagc ctgactacaa aaacatgcat tactgactgc
      121 atgattaaag tcttcctcgg tcgtgatggt ttacctgtaa aggcggagtc atggtgatga
      181 ctaagaaaat aaaatgtgct taccaccttt gcaaaaaaga cgttgaagaa agcaaagcta
      241 ttgaaagaat gcttcacttc atgcacggga ctttatcaaa agacgaaccg agaaaatatt
      301 gcagtgaagc ttgtgccgaa aaagaccaga tggcacacga actttaatta attgactatt
      361 cgaaactgaa tttatgccag aaatggcagg gattcgctca accttaattc aggagaaaaa
      421 catgattacc aattatgaaa ccactgttgt aactaccgat gacattgttc acgaggtgaa
      481 tctggaagga aagcgcattg gctacgtaat taaaacaaaa aataaagaaa ccccattcac
      541 tgtggttgat atcgatggtc catcaggcaa cgtaaaaaca cttgatgaag gtgtcacaaa
      601 aatgtgcctg gttcacatcg gaaagaatct gcccgcagaa aaaaaagccg gatttctggc
      661 aactctgatt gcaatgaaat taaacggtga aatctgaaag aaatagcctg cgtatggcgc
      721 aggctatgaa cagtgtgtat ccggcaagat cattcactga acaaaacgaa ttttaatctg
      781 agttgaggtt aaaaaacaat gagcacaaaa ccactcttcc ttttacggaa agcgaaaaaa
      841 tcatccggtg agcctgacgt cgtcctgtgg gcaagcgacg attttgaatc aacctgtgcc
      901 actctggact acctgatcgt taagtcaggt aaaaaactga gcaactattt taaagctgtt
      961 gccacgaatt ttcctgtcgt taatgacctg ccccctgaag gtgagatcga ttttacctgg
     1021 agtgaacgct atcaactcag caaagactcc atgacctggg aactaaaacc gggagcagca
     1081 ccagacgacg ttcaccacca ggataatgct caggaaacca aagaactggc gggaggcctg
     1141 gaagaaaacg cgcaggcaga cgcccacggg gattgccagg attgcgaagt ctctgtagcc
     1201 actttgcggt tcacacagcg tcttctgcac atttttacgt atgcggccgg ggatcggaaa
     1261 tacctgcatc atgccacccg taaacaacgc gaacacatta ccgctcttga gatggatcag
     1321 gaaaacagct atgtccagaa tctgctgttg gccatacgcg gcatggcaga accgacaact
     1381 ctggataatg ccgccctgct ccgcctgact gatgcaatta aggcagtttt ctctatcacg
     1441 aaaaaacatc agccctatga atttaagaat ttcatttcag cctggctgga caccgaacac
     1501 attgatcgcg gtcttctaac aaaagaatgg cgaaaaggga atcgtgtttc acgcatcact
     1561 cgcacagatt ccggtgctaa tgcaggcggc gggaacctta ccgatcgcgg cgaaggtttc
     1621 gttcatgatc aggcgtcgct ggcccgcgac gtagccactg gcattctggc ccgttcaatg
     1681 gacgtggaca tctataacct tcatccggca cacgctaaac gcattgagga aattatcgct
     1741 gaaaataaac cgcccttttc tgttttccgc gacaaattca tcaccatgcc tggcgggctg
     1801 gattattccc gcgccatcgt ggttgcgtcc gtgaaagaag caccaattgg gatcgaggtc
     1861 atccccgcgc acgtcactga acatctgaac aaagtactga ctgaaactga tcatgccaac
     1921 cctgatccgg aaatcgtgga tattgcctgc ggtcgttcct ctgccccgat gccgcagcgt
     1981 gtaacagaag aagaaaaaca ggatgatgaa gaaaaaccgc aaccatattg cgcaatggca
     2041 gatgaacagg caacggctga aacagtggaa ccggatgcaa ctgaacatca tcaggacacg
     2101 cagccgctgg atgctcagtc acaggtaaat tctgttgatg aaaaatatca gaaactgcgg
     2161 gcagaactcc atgaaacacg gaaaaacatt ccgcccaaaa atcctgtcga tgcagacaaa
     2221 ttactggctg cttcgcgtgg tgagttcgtt gaagggatta gcgacccgaa cgatccgaaa
     2281 tgggtaaagg ggatccagac tcgcgactct gtgtaccaga accagccaga aacggaacaa
     2341 aacacgccag aaactgtaaa aaccagcccg gatgtgaaac aacctgaacc agtagtgcaa
     2401 cagaaaccgg aaatagtctg caatgcctgc ggtcagactg gcggggataa ctgccctgac
     2461 tgtggtgcgg tgatgggcga cgcaacgtat caggaaacct ttaatgaaga aaatctggat
     2521 gaatctcagg aaaaagagcc ggaggaaatg gaaggccctg aacatccgca caatgagaat
     2581 gctggcagcg atctgcatcg cgattgcagt gatgaaactg gcgaagtcgc agatcccgta
     2641 atcgcaggag acatagagcc tggtatttat tacggaattt cgaatgagaa ttaccacgcg
     2701 ggtcccggtg tcagtaagtc tcagctcgac gacattgctg atactccggc gctgtatttg
     2761 tggcgtaaaa atgcccctgt ggacaccaca aagacaaaaa cgctcgattt aggaaccgct
     2821 ttccactgcc gtgtacttga accggaagaa ttcagtaacc gctttatcgt agcacctgaa
     2881 tttaaccgcc gtacaaacgc cggaaaagaa gaagagaaag cgtttctgat ggaatgcgca
     2941 agcacaggaa aaacggttat cactgccgaa gaaggccgga aaattgaact catgtatcag
     3001 agcgttatgg ctttgccgct gggtcaatgg cttgtggaaa gcgccggaca cgctgaatca
     3061 tcaatttact gggaagatcc ggaaacagga attttgtgtc ggtgccgtcc ggacaaaatt
     3121 attcctgaat ttcactggat catggacgtg aaaaccacag cggatattca acgattcaaa
     3181 accgcttatt acgactaccg ctatcacgtt caggatgcat tctacagtga cggttatgaa
     3241 gcacagtttg gagtgcagcc aactttcgtt tttctggttg ccagcacaac tattgaatgc
     3301 ggacgttatc cggttgaaat tttcatgatg ggcgaagaag caaaactggc aggtcagctg
     3361 gaatatcacc gcaatctgcg aaccctggct gactgcctca ataccaatga atggccagct
     3421 attaagacgt tatcactgcc ccgctgggct aaggaatatg caaatgacta agcaaccacc
     3481 aatcgcaaaa gccgatctgc aaaaaactca gggaaaccgt gcaccagcag caattaaaaa
     3541 taacgacatg attagtttta ttaaccagcc atcaatgaaa gagcaactgg cagcagctct
     3601 tccacgccat atgacggctg aacgtatgat ccgtatcgcc accacagaaa ttcgtaaagt
     3661 tccggcgtta ggaaactgtg acactatgag ttttgtcagt gcaatcgtac agtgttcaca
     3721 gctcggactt gagccaggta gcgccctcgg tcatgcatat ttactgcctt ttggtaataa
     3781 aaacgaaaag agcggtaaaa aaaacgttca gctaatcatt ggctatcgcg gcatgattga
     3841 tctggctcgc cgttcaggtc aaatcgccag cctgtcagcc cgtgttgtcc gtgaaggtga
     3901 cgaatttaat ttcgaatttg gccttgatga aaagttaata caccgcccag gagaaaacga
     3961 agatgccccg gttacccacg tctatgctgt cgcaagactg aaagacggag gtactcagtt
     4021 tgaagttatg acgcgcaaac agattgagct ggtgcgcagc cagagtaagg ctggtaataa
     4081 cgggccatgg gtaactcact gggaagaaat ggcaaaaaaa acggctattc gtcgcctgtt
     4141 taaatacctg cctgtctcaa ttgaaatcca gcgtgcagta tcaatggatg aaaaggaacc
     4201 actgacaatc gatccggcag actcctctgt attaaccggg gaatacagtg tgatcgataa
     4261 ttcagaagaa taattcagcc tggcggtgta atgcaccgcc aacgcgagac agtttttatg
     4321 acaaaaatta tgagatatga cgatgttaaa ccatgtccgt tttgtggttg tccatctgtt
     4381 acggtgaaag caatttcagg atattaccgg gcaaaatgca acggatgcga atcccgaact
     4441 ggctatggtg gaagtgaaaa agaagcactc gaaagatgga ataaacgaac tactggaaat
     4501 aataatggag gtgttcatgt ataaaataac tgccactatt gaaaaagaag gtggcactcc
     4561 tactaactgg acaagatatt caaaatctaa attaacgaaa tcagaatgcg aaaaaatgct
     4621 ctcag
//
LOCUS       DBFIFI010000275         4615 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.275, whole genome shotgun sequence.
ACCESSION   DBFIFI010000275 DBFIFI010000000
VERSION     DBFIFI010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4615
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.275"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..607)
                     /locus_tag="N7B39_004495"
     CDS             complement(<1..607)
                     /locus_tag="N7B39_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HGU2424339.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDSYIPE"
     gene            complement(722..797)
                     /locus_tag="N7B39_004496"
     tRNA            complement(722..797)
                     /locus_tag="N7B39_004496"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(762..764),aa:Thr,seq:ggt)
     gene            complement(804..878)
                     /locus_tag="N7B39_004497"
     tRNA            complement(804..878)
                     /locus_tag="N7B39_004497"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(843..845),aa:Gly,seq:tcc)
     gene            complement(995..1079)
                     /locus_tag="N7B39_004498"
     tRNA            complement(995..1079)
                     /locus_tag="N7B39_004498"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1043..1045),aa:Tyr,seq:gta)
     gene            complement(1088..1163)
                     /locus_tag="N7B39_004499"
     tRNA            complement(1088..1163)
                     /locus_tag="N7B39_004499"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1128..1130),aa:Thr,seq:tgt)
     gene            1525..2475
                     /gene="coaA"
                     /locus_tag="N7B39_004500"
     CDS             1525..2475
                     /gene="coaA"
                     /locus_tag="N7B39_004500"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HGU2424340.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2504..3469)
                     /gene="birA"
                     /locus_tag="N7B39_004501"
     CDS             complement(2504..3469)
                     /gene="birA"
                     /locus_tag="N7B39_004501"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HGU2424341.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVTVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3466..4494)
                     /gene="murB"
                     /locus_tag="N7B39_004502"
     CDS             complement(3466..4494)
                     /gene="murB"
                     /locus_tag="N7B39_004502"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HGU2424342.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFDAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLSQFPTAPNYPQADGSVKLAAGWLIDQCQLKG
                     MQMGGVAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1168 a         1163 c         1054 g         1230 t
ORIGIN      
        1 gttccggaat gtaggaatcc aagaagccag ccagttccag gattttcgct tcccactctg
       61 cgtcgccttc cagcgctttc agagcagaac cacgaacgat cggagtgtcg tcgcccggga
      121 agtcgtactg agacagaagt tcacgaactt ccatttcaac cagttccagc agctcttcgt
      181 catcaaccat gtcgcatttg ttcaggaaca cgatgatgta cggaacgcct acctgacgac
      241 ccagcaggat gtgctcacga gtctgcggca tcgggccgtc agtcgcagca actaccagga
      301 tcgcgccgtc catctgagca gcaccggtga tcatgttttt aacatagtcg gcgtgccccg
      361 ggcagtctac gtgtgcgtag tgacgggtcg gggtgtcgta ttcaacgtga gaagtgttga
      421 tggtgatacc acgagctttt tcttccggcg cgttatcgat ctggtcgaat gcgcgagcag
      481 caccgccgta ggttttagcc agtacggtag tgattgcagc ggtcagcgtt gttttaccat
      541 ggtcaacgtg gccgatagta ccgacgttaa cgtgcggttt tgtacgttca aacttttctt
      601 tagacatcga ttgtccctct aagacacgga taaatcggtg atatcaccac atcaaccagg
      661 caacatgccc gacttgttga atgcaataaa cagaaggaaa aaacagggag gagaaaagaa
      721 gtggtgctga taggcagatt cgaactgccg acctcaccct taccaagggt gcgctctacc
      781 aactgagcta tatcagcaca tcttggagcg ggcagcggga atcgaacccg catcatcagc
      841 ttggaaggct gaggtaatag ccattatacg atgcccgcat cctggaactc ggctacctga
      901 ttttcattct gcactgaata tcgagagaag ctctctttat tcgagccggt aagcgaactt
      961 atcgtctcgg gctacgccat cgcgtggccg aaattggtgg tgggggaagg attcgaacct
     1021 tcgaagtctg tgacggcaga tttacagtct gctccctttg gccgctcggg aaccccaccg
     1081 gacttgatgg tgccgactac cggaatcgaa ctggtgacct actgattaca agtcagttgc
     1141 tctacctact gagctaagtc ggcatcaagt agcgcgcatt ctatggagac atgcgagttc
     1201 atgcaactaa aaaattgcat aatttgtttt attggtcaca ttttatgcga cacgatgaag
     1261 aaacagccga atggagggca gattagcaca ctttttcaac atcattgtgc tcaacaatgc
     1321 gctcctgcta aaccataatt ctttttatca gatggaatat ctgtcacatt gcttttcaac
     1381 gatagcttcc tgggagagat tttttcttat tattcctccc catctggtgt taccctcctg
     1441 cccattaacc cattcaacag aactgtgacg cgccatggca aatatcgctt tgccgataga
     1501 gctatgaccg ccagaaacat gcttatgagt ataaaagagc aaacgttaat gacgccttac
     1561 ctacagtttg accgcaacca gtgggcagct ctgcgtgatt ccgtacctat gacgttatcg
     1621 gaagatgaga tcgcccgtct caaaggtatt aatgaagatc tctcgttaga agaagttgcc
     1681 gagatctatt tacctctgtc acgtttgctg aacttctata taagctcgaa tctgcgccgt
     1741 caggcagttc tggaacagtt tcttggtacc aacgggcaac gcattcctta cattatcagt
     1801 attgctggta gtgtcgcggt ggggaaaagt acaaccgccc gtgtattgca ggcactatta
     1861 agccgttggc cggaacatcg tcgtgttgaa ctgatcacta cagatggctt ccttcaccct
     1921 aatcaggttc tgaaagaacg tggtctgatg aagaagaaag gcttcccgga atcgtatgat
     1981 atgcatcgcc tggtgaagtt tgtttccgat ctcaagtccg gcgtgccaaa cgttacagcc
     2041 cctgtttact cgcatcttat ttatgatgtg atcccggatg gagataaaac tgttgttcag
     2101 cctgatattt taattcttga agggttaaat gtcttacaga gcgggatgga ttatccacac
     2161 gatccacatc atgtatttgt ttctgatttt gtcgattttt cgatatatgt tgatgcaccg
     2221 gaagacttac ttcagacatg gtatatcaac cgttttctga aattccgcga aggggctttt
     2281 acagacccgg attcctattt tcataactac gcgaaattaa ctaaagaaga agctattaag
     2341 actgccatga cgttgtggaa agagatcaac tggctgaact taaagcaaaa tattctacct
     2401 actcgcgagc gcgccagttt aatcctgacg aaaagtgcta atcatgcggt agaagaggtc
     2461 agactacgca aataatttgc aggggagcta atgctcccct ttcttatttt tctgcactac
     2521 gcagggatat ttcaccgccc atccagggtt ttattattcc atcctgctca agcaataaag
     2581 cgccttgttt gtctattccg cgtgaaatgc caaatatttc tttatcacca atgataagtt
     2641 tcactgggcg attaataaaa ttatccagct tttcccagcg cgaaagataa ggtgccaatc
     2701 cttcttgttc gaagagttcc agcgccgcgc gtaattcacg tattagcatg gccgccaacg
     2761 tattacgatc gagattgatc cccgcttcct gcagcgtgat ccacccctga ttaacgacac
     2821 tctcttcaac acgacgcatt gccatgttga tcccggctcc aatgactatt tgcgccgcat
     2881 cgccggtttt gccagtcagc tccacaagaa tgcctgccag cttgcgatcc tgcagataga
     2941 ggtcattagg ccatttgaca cgaactttat ctgctcccag cttgcgtaat acttccgcca
     3001 tcacgatacc gataaccaga cttaaaccaa tcgccgccgc cgggccttgt tccagacgcc
     3061 agaacatcga caaatataag tttgcgccaa aaggcgaaaa ccatttccga ccccggcgac
     3121 cacggccagc ctgctggtat tctgcaacgc aggcatcgcc cgatttaagc tctccgatac
     3181 gatcaagaag gtactgattc gtggagtcaa tcactggcag cacagttaca ctaccgccat
     3241 ccagctgacc caatatctgt tcagcattaa gtaactggat aggctcaggc aggctgtatc
     3301 ctttacccgg aacggtaaag acatcaacgc cccagtcacg cagtgtctga atgtgtttat
     3361 taatagccgc ccggctcatt cctaacgttt cacccaactg ctcacctgag tgaaattcgc
     3421 cattcgctaa cagcgcgatt aatttcagtg gcacggtgtt atccttcatg aaattgtctc
     3481 cactgcgctc acttcacctg atgcaccaat aaagcggact tcaggctcaa gccagacatt
     3541 aaatttttca cccacttttt gtcttacatg gtgtgccagt tgcaccacat cttcgctttt
     3601 tgcattgtct tcattaatga gaactaacgc ctgttgacgg tgcaccgcaa ccccacccat
     3661 ttgcatccct tttagctggc actgatcgat aagccaacct gctgccagtt ttactgaacc
     3721 atccgcctgg gggtaatttg gtgctgttgg aaattgtgac agtaatgctt tagccgtttc
     3781 ggcagataca acagggtttt tgaagaaact accggcattg ccattcactt ttggatcagg
     3841 gagtttggtg gtgcgcatat gacacaccgc atcaaatact tgctgtggcg ttactgttgt
     3901 aggatccaga cgagttaagt caccatacgt tagtacaggt tgccactctt ttggcagacg
     3961 cagacctacg gctacaatgg cgaagcggtc ctggtattca tgtttaaaaa tactgtcgcg
     4021 atagccaaaa cggcactctt tggcagttaa gcgcacttgc ttgcctgtcg ccagttcaac
     4081 acaatcaaca taagcgcaaa ctcgctgtaa ttctacgcca taagcaccaa tattctggat
     4141 aggtgatgag ccgacacaac caggaattaa tgccagattt tccagaccag gcataccttc
     4201 ctgcaaagtg tattttacca ggcgatgcca gttttctccg gctcctacat gtaaatacca
     4261 cgcatcaggt tcatcatgaa tttcgatacc tttgatccgg ttgatgatca ccgtgccgcg
     4321 atagtcttcc agaaaaagta cattacttcc ttcacccaga ataagaacgg gttgcccttc
     4381 tgcggttgca tgctgccagg cattgagtag ttgttgttcg tcttcggcac atacaatgtg
     4441 ctgagcatta tgatcaatgc caaatgtgtt ccagggtttt aaggagtggt tcatagctgc
     4501 tttcctgatg caaaaacgag gctagtttac cgtatctgtg gggggatggc ttgtagttat
     4561 gacgacaggg agagtttgta gaaacgcaaa aaggcccagt cttccgaccg ggcct
//
LOCUS       DBFIFI010000276         4582 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.276, whole genome shotgun sequence.
ACCESSION   DBFIFI010000276 DBFIFI010000000
VERSION     DBFIFI010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4582
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.276"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..1352
                     /locus_tag="N7B39_004503"
     CDS             <1..1352
                     /locus_tag="N7B39_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HGU2424343.1"
                     /translation="SEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIGIG
                     FVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPIAI
                     LVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKLGD
                     WFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKFGP
                     FGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAKQA
                     RSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGDLA
                     TIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGGMV
                     ASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKVVLAE"
     gene            1400..2440
                     /gene="gatD"
                     /locus_tag="N7B39_004504"
     CDS             1400..2440
                     /gene="gatD"
                     /locus_tag="N7B39_004504"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HGU2424344.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            2546..3319
                     /locus_tag="N7B39_004505"
     CDS             2546..3319
                     /locus_tag="N7B39_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGU2424345.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRVHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSDVDKLALQKTRVKLITV"
     gene            complement(3401..4300)
                     /gene="yegS"
                     /locus_tag="N7B39_004506"
     CDS             complement(3401..4300)
                     /gene="yegS"
                     /locus_tag="N7B39_004506"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HGU2424346.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
BASE COUNT         1114 a         1029 c         1204 g         1235 t
ORIGIN      
        1 tttcagaagt catgcgttat attctcgatc tcggccctac ggtgatgttg ccgattgtca
       61 tcattatttt ttctaaaatt ttaggcatga aggcaggcga ttgctttaaa gcgggtctgc
      121 atatcgggat tggctttgtt ggcattggcc ttgtgattgg cttaatgctg gattccattg
      181 gcccggcggc gaaagcgatg gcggaaaatt tcgacctgaa tctgcatgtg gtcgatgtcg
      241 gctggccggg ctcttcacca atgacctggg cgtcgcaaat tgcgctggtg gcgattccga
      301 ttgcgattct ggttaacgtg gcgatgttac tgacccgtat gacgcgggtg gtaaatgttg
      361 atatctggaa tatctggcat atgaccttca ccggcgcgtt gctgcatctg gcaaccggtt
      421 catggatgat agggatggca ggtgtggtaa ttcacgcggc gtttgtttat aagctcggcg
      481 actggtttgc ccgcgatacc cgaaatttct ttgagctgga aggcattgct attccgcacg
      541 gtacgtcggc gtatatgggg ccgattgcgg tgctggtcga tgctatcatc gagaaaatcc
      601 caggcgttaa ccgtattaaa tttagcgccg acgatattca gcgcaaattt ggcccgtttg
      661 gcgagcctgt caccgtgggt tttgtgatgg ggctgattat cggcatcctc gcgggttacg
      721 atgtcaaagg tgtattgcag ctggcggtaa aaacggcggc ggtgatgctg ctaatgccac
      781 gggtgattaa acccatcatg gatggtttaa cgcccatcgc taagcaggcc cgtagtcgtt
      841 tacaggcgaa gttcggcggt caggagttcc tgattggcct ggatcccgca ttactgctgg
      901 ggcatacggc ggtggtatcg gcaagcctga tttttattcc gctcaccatt ttaattgctg
      961 tttgtgttcc tggtaatcag gtactgccgt ttggcgatct tgccactatc ggcttcttcg
     1021 tggcgatggc ggtcgcggtg catcgtggaa atctgttccg caccttaatc tcgggtgtca
     1081 tcattatgag catcaccctg tggatcgcga cgcaaactat tggtttgcac acccaactgg
     1141 cggctaatgc tggggcgtta aaagccgggg gtatggtggc ttcaatggat cagggcggtt
     1201 ctcccattac ctggttactg attcaggttt tctccccgca aaatattccc ggtttcatta
     1261 ttatcggcgc aatttatctt accggtattt tcatgacctg gcgtagagcg cgtggcttta
     1321 ttaaacaaga gaaagtcgtt ctcgcagaat aatttttacc tgagggggag gttatccccc
     1381 ttaacaatta ggagttttta tgaaatcagt ggtgaatgat actgatggta tcgtgcgcgt
     1441 tgcagaaagc gtcattcctg aaattaaaca tcaggatgag gtgcgggtaa aaattgccag
     1501 ctcgggctta tgtggttccg atttacccag gatatttaaa aatggtgcac attattatcc
     1561 aataacgtta ggccatgaat ttagcggcta tattgatgcg gtaggatccg gtgttgatga
     1621 tttacaccct ggcgatgcgg ttgcctgtgt gccgttatta ccctgtttta cttgtccaga
     1681 gtgtttgaaa gggttttatt cccagtgcgc aaaatatgat tttattggct cacggcgtga
     1741 tggtggattt gccgaatata ttgtcgttaa gcgaaaaaat gtctttgctc tacccacgga
     1801 tatgcctatt gaggatgggg cttttattga gccgattacc gttggcctgc atgcttttca
     1861 tttagcgcaa ggttgtgaga ataaaaacgt tattattatt ggtgccggaa ccattggcct
     1921 gctggctatt cagtgcgctg tcgcgctggg agcaaagagt gtgacggcta tcgacattag
     1981 ctcagaaaaa ctggcactgg caaaatcttt cggtgcgatg caaacattta acagtagcga
     2041 aatgagcgcg ccgcaaatgc agagcgtttt acgcgaactg cgctttaatc agcttatcct
     2101 cgagacggct ggcgtaccgc aaactgtcga actggcggta gagattgccg gtcctcatgc
     2161 ccaactggca ctggtgggca cgttgcatca ggatctgcat ttaacatcgg caacgtttgg
     2221 caaaatattg cgtaaagagc tgacggttat cggcagttgg atgaactact ccagcccttg
     2281 gccggggcag gagtgggaaa cggcgagccg gttgctgaca gaacgtaagt taagcctgga
     2341 gccattaatc gctcaccgtg gaagctttga aagcttcgcc caggcggtgc gtgacatcgc
     2401 tcgtaatgct atgccgggca aagtgttgct cattccctga aaccgcgggc cagcgtgatg
     2461 ctggcccggt attgtgcaaa acagatcatt caccaatggt cccccttcgt ttacactagc
     2521 cacaattgaa tatgggtaaa tgacgatgaa ttcattcgag cgaaggaata agatcatcca
     2581 attagtgaat gaacagggaa ccgtgcttgt tcaggatctg gcgggagtat ttgctgcctc
     2641 ggaagcgaca atccgtgccg atttgcgctt tctcgaacaa aaaggcgtgg ttacgcgcgt
     2701 tcatggcggt gcggcgaaaa taatgtctgg taatagtgaa accgagaccc aggaagtcgg
     2761 gtttaaagag cgatttcagc ttgccagcgc gccaaaaaac agaatagcgc aggcggcagt
     2821 caaaatgatc cacgaaggga tgaccgttat tctcgacagc ggaagtacga caatgcttat
     2881 cgccgaagga ttaatgaccg ccaaaaatat cacggtgatt accaacagtc tcccggcagc
     2941 gtttgccctt tccgaaaata aagacattac tctggtcgtc tgtggtggca cggtccgcca
     3001 taaaacgcgc tcgatgcatg gttccattgc cgagcgttca ttgcaggata ttaatgccga
     3061 tttaatgttt gtcggtgctg atggtattga tgcggttaat ggtattacga cctttaatga
     3121 aggttattcg attagcgggg cgatggtcac cgccgcaaat aaagtgattg ccgttctcga
     3181 ttcatcgaaa tttaatcgtc gcggttttaa tcaggtactg ccaatcgaaa aaattgacat
     3241 tattattacc gatgatgccg tgtcagatgt ggataaactg gcattgcaga agacacgggt
     3301 aaaattaatt acggtgtagt attgttctgt atgccggata aggtgtttac accgcatccg
     3361 gcattacgga ttctccctgg ttcaagagag aatagtttga ttaacgcaat aatggacaat
     3421 ctggtggtaa tcgacaacgc aacgccgccg gaagtatttc aatatggaag ttttgcccac
     3481 tcaacggttc gccatcaaga ttaaaagtga tttcgtgtgg ggcttgtata tcaaaccacg
     3541 acgaagcgcc ttcgataata ttcgggttat cttcgtcaga ttttaaggtt gatacgagag
     3601 ccggaagaat ttcatcgccg gtaaaaatgc gcagttgcag caagccatcg ttaattaacg
     3661 cgttcgggca caattgttga ccgccaccgg cctgacgccc gttaccaata ccaatgacca
     3721 gggcgtcacc ttgccagtga aagttttcac cgcggatttc acaacggtcc ggttgcagag
     3781 tatccatgcg cattaagcca tgaatgatgt aagagacgcc acccagcgcg gcttttaatt
     3841 tttccggcgt ttctgtggta atacgcgtcc caaatccgcc tgtcgccata ttaataaaac
     3901 aggtttgttt gttgacctgc gccatatcta tcgcaatggc gttaccggca attgccagtt
     3961 tcagcgcctt atccagtgcc tcaggaatcc ctacactggt ggcaaaatca ttggcggttc
     4021 ctaatggcaa aattcccagc gcgggtatgt catccccctc acactgaatc aacgccgtag
     4081 aaacttcatt aatggtgcca tcgccaccac cggcaatcac cgttgcgacg cccaacttcc
     4141 gggcctcctc tacatatcgt gcggcatcgc ctttctccca ggtgacccgc acatggatcg
     4201 tcattccttc ctcacgcaac agcataattg cttcgcgcaa gggtagattg tcagtacttt
     4261 tgccattaag aatcagtaag ctggcgggaa attctgccat gatcgtatgt gcctttatga
     4321 ttggtctata gatagtgtag agcagaaaag aaaaggtgga gtcagaacag gatgaaagtc
     4381 ggaggataaa tagtcagttc aagtatggag gattcagtgg ttaccaccag gagcttgcaa
     4441 tggggacagc gaatactaac ggcagccacc gataaatttc aaaaaagagc atatacctaa
     4501 tattcaacta aacagtggct tcttcaatat aatatattaa agcctccatg gagttacccg
     4561 gaagggcctc catgtccgta at
//
LOCUS       DBFIFI010000277         4506 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.277, whole genome shotgun sequence.
ACCESSION   DBFIFI010000277 DBFIFI010000000
VERSION     DBFIFI010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.277"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            53..346
                     /gene="bor"
                     /locus_tag="N7B39_004507"
     CDS             53..346
                     /gene="bor"
                     /locus_tag="N7B39_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serum resistance lipoprotein Bor"
                     /protein_id="HGU2424347.1"
                     /translation="MKKMLLATALALLITGCAQQTFTVQNKPAAVTPKETITHHFFVS
                     GIGQKKTVDAAKICGGAENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSQ"
     gene            complement(437..619)
                     /gene="rzoD"
                     /locus_tag="N7B39_004508"
     CDS             complement(437..619)
                     /gene="rzoD"
                     /locus_tag="N7B39_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HGU2424348.1"
                     /translation="MRKLKMMLCMMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(836..1312)
                     /locus_tag="N7B39_004509"
     CDS             complement(836..1312)
                     /locus_tag="N7B39_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HGU2424349.1"
                     /translation="MQNLNPQRKAFLDMLAWSEGTDNGRQPTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLHPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVRESEV"
     gene            complement(1299..1604)
                     /locus_tag="N7B39_004510"
     CDS             complement(1299..1604)
                     /locus_tag="N7B39_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HGU2424350.1"
                     /translation="MHEKESLAGAFWLVLLIIAGWGGLVRYLIDVKQSKATWSWINAL
                     AQIVVSGFTGVIGGLISIESGFSIYMILATAGISGAMGSVALTYFWERLTGVKNAKS"
     gene            complement(1926..2615)
                     /locus_tag="N7B39_004511"
     CDS             complement(1926..2615)
                     /locus_tag="N7B39_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HGU2424351.1"
                     /translation="MNTQYLQYVREQLIVATADLSGATKGQLEAWLEHAQFDTGTYKR
                     KKPRILDEVTGRMITLDNPPISGKQSYAKGSSIALVSQVEFSTSSWRRAVLSLEEHQK
                     AWLLWSYSESVRWEHQVTITQWAWSEFKTLLGTRKIAGKTLERLKKLIWLAAQDVKNE
                     LAGRKTYEYQELASLVGVTSKNWSETFTERWVAMKHIFLQLDSQALLLLTKTRSKQKT
                     TFSQQDIAKLD"
     gene            complement(2612..2752)
                     /locus_tag="N7B39_004512"
     CDS             complement(2612..2752)
                     /locus_tag="N7B39_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HGU2424352.1"
                     /translation="MMFESYMAERLRHRWMRLRLYRFPGSVLTDYRILKNYAKTLKGA
                     AA"
     gene            complement(2749..3111)
                     /gene="rusA"
                     /locus_tag="N7B39_004513"
     CDS             complement(2749..3111)
                     /gene="rusA"
                     /locus_tag="N7B39_004513"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HGU2424353.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHISAEGQAYRDNVARIIKG
                     SMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGKLELTITELGNE"
     gene            complement(3108..3398)
                     /gene="ybcO"
                     /locus_tag="N7B39_004514"
     CDS             complement(3108..3398)
                     /gene="ybcO"
                     /locus_tag="N7B39_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HGU2424354.1"
                     /translation="MADLRKAARSRECQVRIPGVCNGNPETSVLAHIRLTGLCGTGTK
                     PPDLIATIACSACHDEIDRRTHFVDAAYAKECALEGMARTQVIWLKEGVIKA"
     gene            complement(3391..3561)
                     /gene="ninE"
                     /locus_tag="N7B39_004515"
     CDS             complement(3391..3561)
                     /gene="ninE"
                     /locus_tag="N7B39_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HGU2424355.1"
                     /translation="MATPLIRVMNGHIYKVPNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            complement(3561..4016)
                     /gene="ybcN"
                     /locus_tag="N7B39_004516"
     CDS             complement(3561..4016)
                     /gene="ybcN"
                     /locus_tag="N7B39_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbcN family protein"
                     /protein_id="HGU2424356.1"
                     /translation="MNLPQDGIKLHRGNFTAVGQQIQTYLEDGKCFRMVLKPWRERRS
                     LSQNALSHMWYSEISEYLISRGKSFATSAWVKDALKHTYLGYETKDLVDVVTGEITTI
                     QSLRHTSDLGTGEMYVFLCKVEAWAMNIGCHLTIPQSCEFQLLRDKQEA"
     gene            complement(4013..4111)
                     /locus_tag="N7B39_004517"
     CDS             complement(4013..4111)
                     /locus_tag="N7B39_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004141699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424357.1"
                     /translation="MRPQSELLTLSQMQKCTCDVLHPAFDLCGGEA"
BASE COUNT         1121 a         1133 c         1049 g         1203 t
ORIGIN      
        1 attttctaca catacgattc tgcgaacttt aaaaagcatc gggaataaca ccatgaaaaa
       61 aatgctactc gctactgcgc tggccctgct tattacagga tgtgcccaac agacgtttac
      121 tgttcaaaac aaaccggcag cagtaacacc aaaggaaacc atcacccacc atttcttcgt
      181 ctctggaatt gggcagaaga aaactgtcga tgcagctaaa atttgtggcg gcgcagaaaa
      241 tgttgttaaa acagaaaccc agcaaacatt cgtaaatgga ttgctcggtt ttattacttt
      301 aggcatttat actccgctgg aagcgcgtgt gtattgctca caataattgc atgagctgcc
      361 catcgatatg ggcagctcta tctgcactgc tcattaatat acttctgggt tccttccagt
      421 tgtttttgca tagtgatcag cctctctctg agggtgaaat aatcccgttc agcggtgtct
      481 gccagtcggg gggaggctgc attatccacg ccggaggcgg tggtggcttc acgcactgac
      541 tgacagactg ctttgatgtg caaccgacga cgaccagcgg caacatcatc atgcagagca
      601 tcattttcag ctttcgcatc agctaactcc ttcgtgtatt ttgcatcgag cgcagcaaca
      661 tcacgctgac gcatctgcat gtcagtaatt gccacgttcg ccagcttcag ttctctggca
      721 tttttgtcgc gctgggcttt gtaggtaatg gcgttatcac ggtaatgatt aacagcccat
      781 gacaggcaga cgatgatgca gatgagcaga gcggagataa tcgcggttat tctgctcata
      841 cctcactctc tctgaccgtt ccgccagctt ctttgaattt tgcaatcagg ctgtcagcct
      901 tatgctcgaa ctgaccataa ccagcgcccg gcagtgaagc ccagatatta ctgcaacggt
      961 cgattgcctg acggatatca ccgcgatcaa tcataggtaa agcaccacgc tctttaatct
     1021 gctgcaatgc cactgcgtcc tggcttttgg gggagaagtc tttcaaacca agctgtttac
     1081 ggtaagcatc ccaccagcgt gaaagaagct gataacgtcc ggcggctgtt gatttgagtt
     1141 tggggtgtag cgtgacaagt ttgcgagggt gatcggagta atcagtaaac agttcgccgc
     1201 caacaataac atcataaccg tggttacgtg tcggttgtcg cccgttatcc gttccttctg
     1261 accacgccaa catatcgagg aaggctttac gctgaggatt aagattttgc atttttcacc
     1321 cctgtcagtc gttcccagaa gtacgtcagt gcaaccgaac ccatcgcacc actaatcccc
     1381 gctgtcgcga gaatcatgta aatactgaat ccactttcga tgctgatcag gccaccaata
     1441 acaccggtga atcctgatac cactatttga gccagagcat ttatccaact ccacgttgct
     1501 ttactctgct tcacatctat caggtagcgg accagaccgc cccaacctgc gatgatcagc
     1561 aaaacgagcc agaacgctcc ggcaaggctc tctttttcgt gcatatgaat agccaatgtt
     1621 tcgccgccga caaaaggccg ggacgttaaa tgtcagaaat caggctcacg gggtaattta
     1681 acgacaaagc acggagttga tgctccccac aagcctggaa taaaaaagcc agcatgtagc
     1741 tggcaacaga gggctaagca atatcaactc aacagctgaa gaccccctgg ctggggtagg
     1801 ttggaaggct actcaccgtt cagaaacaga aaagcccaag gctttaaacc tcgggcttga
     1861 atttggatta ctgccagtgc gtacaacatt ggcaaaatat cagatttaca taaaatatat
     1921 gatttttaat ccagttttgc aatatcttgc tgtgaaaatg tggtcttttg ttttgaacgt
     1981 gttttcgtta aaagcaataa agcttggcta tcaagctgta gaaaaatgtg tttcattgca
     2041 acccagcgtt cagtaaatgt ctcagaccag ttttttgatg tcactcccac cagtgatgcc
     2101 agctcctggt attcataggt cttacgccct gccagctcgt tcttcacatc ctgtgccgcc
     2161 agccagatca acttcttcaa acgttccagt gtcttacctg caattttcct ggtacccaac
     2221 agagtcttaa actcgctcca tgcccactgc gttatggtga cctgatgttc ccagcgaaca
     2281 ctttcgctgt aactccacag caaccacgct ttctgatgtt cttcgagaga cagaaccgcg
     2341 cggcgccatg acgaggttga gaactcaacc tggctgacca gtgcaatgga tgaacctttt
     2401 gcgtacgact gcttaccgga aatcggcgga ttatccagcg taatcatcct gccagttacc
     2461 tcatccagaa tgcgcggctt ctttcgtttg tatgtaccag tatcaaattg tgcatgctcc
     2521 agccaggctt caagctgtcc tttcgttgct ccgctcaaat cagcggtagc cacaatgagt
     2581 tgctcgcgga catactgtaa atattgggta ttcatgcggc agctcctttc agtgttttgg
     2641 cgtaattctt cagtatccgg taatcggtca aaacagaacc aggaaaacga tataagcgca
     2701 ggcgcatcca gcggtggcga agacgttctg ccatataaga ctcaaacatc attcattccc
     2761 cagttcggtg atggtcagct ccagcttccc acctttggta acgggcatct tcacaacacg
     2821 gtaatcaacg acctgagcat catccagcca gaaacctgct ttggtgagtg cgtcaaaagc
     2881 ggctttttgc agattatcca ggtcacggcg acggcgatcc ggcatgtggc actcaatacg
     2941 gattttcact ggcatagcca ggccgatatc cagcatggag cctttgatga ttcgggcgac
     3001 gttatcacgg tatgcctgcc cttctgcgct gatgtgcgtg cgcccgcgat tatgccggta
     3061 gtagcgatta ttgctcggag gccagggtaa tgtgatgctg taggtattca cgccttaata
     3121 accccctctt tcagccagat aacctgtgtt ctcgccatac cttccagcgc gcattctttt
     3181 gcatatgcag catcgacaaa atgtgtgcgg cggtcgattt cgtcgtggca ggcagaacat
     3241 gcaatggtgg caatcaagtc tggcggtttg gtaccggtgc cgcacaatcc agtcagccgg
     3301 atatgtgcca gtacagacgt ttcagggttg ccattacata cgccagggat tcttacttgg
     3361 cattcccgac tacgcgctgc ttttctcaaa tcagccatga ttcctccttg ctgccagtcg
     3421 caaccatttt ttatcaacca ggctggcggt atatccgagc agtgttggta tttcggatgg
     3481 cttcagctca ggtttacgct tacgacgatt tggtactttg tagatgtgtc cgttcatgac
     3541 acgaataagc ggtgtagcca ttacgcctcc tgcttgtcac gcagcagctg gaactcgcag
     3601 ctctgtggaa tagtcaggtg gcagccaata ttcatcgccc aggcttcaac cttacacagg
     3661 aagacataca tctctccggt accaagatcg gaggtatggc gtaacgactg gatagtagtg
     3721 atttcgccgg ttacgacatc aaccaggtcc ttggtttcat aaccgaggta tgtgtgtttg
     3781 agagcatctt ttacccatgc tgaagtagcg aacgatttcc ccctgctgat gaggtattca
     3841 ctgatttcgc tgtaccacat gtggctgagt gcattctggg aaagactgcg tctctcgcgc
     3901 cacggtttaa gcaccatgcg aaagcatttg ccgtcttcca gataagtctg gatctgctga
     3961 ccgacagcgg tgaagttacc gcgatgtaat ttgatgccat cttgtgggag gttcacgctt
     4021 cacctccgca gagatcaaac gctggatgca agacatcgca ggtgcatttc tgcatctgtg
     4081 acaaggtaag gagttcagat tgtggtcgca tttaagtccc cttaaatgcg cagaagtcac
     4141 cggagttgtt caagctccgg tgacattatt atggaaggtt gatactaaaa aatcaaaaat
     4201 tgaatatcat tggcgatagt ctagcttgtg atatccacaa gctacttcaa tgaagcgacg
     4261 agtaacattt tgtgtcatcc atgttttttc agttaagtgc cacacccagt tcaaaatttg
     4321 ctgatgtgtt gaacatgtac taagcgggat gttgtattca taagcaacat tgattccgca
     4381 aaatcggtgt cgtgtcatga ccgattcacg ttgacctgcg ccctgttgag tgcctgggga
     4441 tattcttgct cgatagttaa tatcatagta gttactttac tttcatacca tagcacggtt
     4501 gaaaaa
//
LOCUS       DBFIFI010000278         4490 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.278, whole genome shotgun sequence.
ACCESSION   DBFIFI010000278 DBFIFI010000000
VERSION     DBFIFI010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.278"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            45..269
                     /locus_tag="N7B39_004518"
                     /pseudo
     CDS             45..269
                     /locus_tag="N7B39_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            287..1615
                     /gene="dsdA"
                     /locus_tag="N7B39_004519"
     CDS             287..1615
                     /gene="dsdA"
                     /locus_tag="N7B39_004519"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HGU2424358.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLEDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAEQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMEQYLAKGR"
     gene            complement(1723..3261)
                     /gene="emrY"
                     /locus_tag="N7B39_004520"
     CDS             complement(1723..3261)
                     /gene="emrY"
                     /locus_tag="N7B39_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HGU2424359.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     SINLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVIFLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTETIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(3261..4424)
                     /gene="emrK"
                     /locus_tag="N7B39_004521"
     CDS             complement(3261..4424)
                     /gene="emrK"
                     /locus_tag="N7B39_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HGU2424360.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKNEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
BASE COUNT         1253 a         1024 c          997 g         1216 t
ORIGIN      
        1 tgccaactgg catcgccaac ctggagaaga aaatataaat atggatcatt gtgctgccgc
       61 tgtatcccga catcagcccg gaaattattg cgattgctat cggttcaggc gcgattggct
      121 gcacgatcgt tacggactcg cttttctggc tggtgaagca atattgcggc gctacgctca
      181 atgaaacatt taaatactat acgacagcga catttatcgc ttcagtcgtc gctctggcgg
      241 gcacattcct gctgtcattt atcatctaag cgcaaagaga cgtactatgg aaaacgctaa
      301 aatgaactcg ctcatcgccc agtatccgtt ggtaaaggat ctggttgctc ttaaagaaac
      361 cacctggttt aatcctggca cgacctcatt ggctgaaggt ttaccttatg ttggcctgac
      421 cgaacaggat gttcaggacg cccatgcgcg cttatcccgt tttgcaccct atctggcaaa
      481 agcatttcct gaaactgctg ccactggggg gattattgaa tcagaactgg ttgccattcc
      541 ggctatgcaa aaacggctgg aaaaggaata tcaacaaccg atcagcgggc aactgttact
      601 gaaaaaagat agccatttgc ccatttccgg ctccataaaa gcacgcggcg ggatttatga
      661 agtcctggca catgcagaaa aactggctct ggaagcgggg ttgctgacgc ttgaagatga
      721 ctacagcaaa ctgctttctc cggagtttaa acagttcttt agccagtaca gcattgctgt
      781 gggctcaacc ggaaatctgg ggttatcaat cggcattatg agcgcccgca ttggctttaa
      841 ggtgacagtg catatgtctg ctgatgcccg ggcatggaaa aaagcgaaac tgcgcagcca
      901 tggcgttacg gtcgtggaat atgagcaaga ttatggtgtt gccgtcgagg aaggacgtaa
      961 agcagcgcag tctgacccga actgtttctt tattgatgac gagaattccc gcacgttgtt
     1021 ccttgggtat tccgtcgcag gccagcgtct taaagcgcaa tttgccgagc aaggtcgtat
     1081 tgtcgatgct gataaccctc tgtttgtcta tctgccgtgt ggtgttggcg gtggtcctgg
     1141 tggcgtcgca ttcggactta agctggcgtt tggcgatcat gttcactgct tttttgccga
     1201 accaacgcac tccccttgta tgttgttagg cgtccataca ggattacacg atcagatttc
     1261 tgttcaggat attggtatcg acaaccttac cgcagcggat ggccttgcag ttggtcgcgc
     1321 atcaggcttt gtcgggcggg caatggagcg tctgctggat ggcttctata cccttagcga
     1381 tcaaaccatg tatgacatgc ttggctggct ggcgcaggaa gaaggtattc gtcttgaacc
     1441 ttcggcactg gcgggtatgg ccggacctca gcgcgtgtgt gcatcagtaa gttaccaaca
     1501 gatgcacggt ttcagcgcag aacaactgcg taatgccact catctggtgt gggcgacggg
     1561 aggtggaatg gtgccggaag aagagatgga gcaatatctg gcaaaaggcc gttaataacg
     1621 tttcaacgca gcatcgcaat cctttccctg ggtgagcgat gctgccgatg gcgcagactt
     1681 aagatcccca gtcttacccg ctataacccc ctgcttttac catcacccaa cgcctttcgc
     1741 tgtaaacggc ggtttcgcaa accaaaccaa aaccgtaagt aagataaaag caatagccgc
     1801 catacggaaa atctcatttg cagaaataga aagtgactgc tgggttattt cattattaat
     1861 ttcattaaga actcctgaaa gcgaaccata gtatttatcc ataatttgtg acgatgaatt
     1921 aaacacgggg ttaaattgat cgatggtttc tgtcaactga ctatggtgta acgattcgcg
     1981 tcgtccccac agcgtcattg tcaacgacgt accaactgat cctgacaagg tacgaaaaaa
     2041 attactcata ctcgaggcat tggcaaattt attatctggc aagcctgaaa acgaaatcgt
     2101 tgttaagggt aaaaagaaac aggcaacggc gaatccctga aaaaactgcg gcaaaatgat
     2161 acctgtaaaa tcaatcgttg gcataaatgt cacagaacgc cagtaatagc aaaccgcata
     2221 catcaaaaaa ctgaatgtca ccaacacccg catgtctatt ttgttgccat aacgtcctat
     2281 caaaggtgaa attaatagtg gcatgatgcc gatgggcgca taagcaagtc cggcccatat
     2341 cgcattatac cccatcgttt cctggagtaa ctgcggcata aggacgatcg ctccagagta
     2401 aaataaatac gcgcatgtga tactcacaat accaatggtg aagttacggg acttaaacaa
     2461 actgagatca agaatcgggt tctctgaggt cgactcccaa atgactaaag agatcagaaa
     2521 aataactgat actactgtta atattattat tgtactcgag ttgaaccaat ccagatcgcg
     2581 ccctttatca agcataattt gcaagccacc aacaccgagc actaacaggg tcagtcctgg
     2641 tagattcatt ttgaccggtg aagtctcagt ttctcttcct ttaagtaagg ttaagcataa
     2701 tgtcaggacg ataatcccca tagggacatt gattaaaaat atccaacccc agctaaagtt
     2761 atcacaaata taaccgccca atatcggccc acatatcgga gcgataatca cggtcattga
     2821 ccataatgcc agagcaaatg ttcttttttc tggtggataa ttccttaata ataaactctg
     2881 tgacagtgga attaacggcc ctgccattaa cccctgaacg actctaaaaa atatcagcac
     2941 atcaagattg atcgataaac tacacattaa ggaagacagc gaaaaaaaag tgactgaaag
     3001 taaaaataac cttaattcgc ctattctttg tgccagcctg ccggtaacag ggatcgcaat
     3061 ggcatttgct acgccaaacg aggtgataac ccaggtgcct tcgtctgttg atgctcccag
     3121 aaagccagat attgtcggta ttgcgacgtt agaaatagtg gaatccaaca tttgcataaa
     3181 cgtcgctaat gacaatgcaa tagtgacgca ccataacgtc ccaccggtta atggtgccgg
     3241 agttgattta gtgattgcca ttaaagttgt ccattatgcg aaataatgtt gctaatttct
     3301 ttttctatcg gactggtatc gataactaaa gccttactgg tataagccgg catggacgtc
     3361 acggttgaag ccagctcagg catctcagca atgtcttcgt tcttcgtatc aatagttgca
     3421 gtcatcgata aaccaatgcg caaggggtgt tccatgagtt cttttggatc aagagaaact
     3481 tcaaccggta cacgctgaac gattttgatc cagttccctg tcgcattttg tgcaggtaat
     3541 aaggagaacg cattgccggt tcccatattg atccctgtca cccggccatg aaacacaaca
     3601 ttctcaccat aaagatcgct gataatattg accgattgac caatccgtac atccgtgagt
     3661 tgtgtttctt taaagttagc attaacccac atttgacgtg ccggtacgac agccattaac
     3721 gattgtccgg gactcactgt ttcgccgacc tgaacacttc tctgggcaat atagccggta
     3781 accggactct taatatccgt acgtttaagc gccaaccagg cttctttggt tgcatccgca
     3841 gcttcaacga cttgtggctg acggtttaat ggtgtgttca ttactaaagc tttattcgct
     3901 ttataagcct ggatagcggc attcaatgcc gctttgctac ttattaacgt atctttggta
     3961 tgctccagcg tttcttttga aataaccccc tgcttcgcta aaggcacacg acggttataa
     4021 tcttctaaag attgttgata ctgaatacgt gctgaagcga cttcggcact gtattgttta
     4081 tcctgtaagt acagtttatt cgtttgccga acaatatttg ccagattatt tttagcttta
     4141 ttgagtgcga tagtggcatc agttttatcc agtgaaacta aaatgtcacc ctgtcgaacg
     4201 tagttcgtat ctttatgatt aacgacagtg acactacctg agacttgtgc agaaattgga
     4261 tctgcattcc ccgtgacata ggcgtcatct gtactaatca tgtcttctaa ttccattgac
     4321 caataggcat aagcacctga aaacgcaata aataaaacta tcgccaataa agaaaagtat
     4381 tttcttctgt tagaatgttt tttatttgaa ttaatctgtt ccactattat ctctcatttc
     4441 gcatagatga aatttatgaa ttgaatgaaa agagtattca ttcttcatat
//
LOCUS       DBFIFI010000279         4451 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.279, whole genome shotgun sequence.
ACCESSION   DBFIFI010000279 DBFIFI010000000
VERSION     DBFIFI010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4451
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.279"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            103..1137
                     /gene="tqsA"
                     /locus_tag="N7B39_004522"
     CDS             103..1137
                     /gene="tqsA"
                     /locus_tag="N7B39_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HGU2424361.1"
                     /translation="MAKPLITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSTLNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFKQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(1162..2550)
                     /gene="pntB"
                     /locus_tag="N7B39_004523"
     CDS             complement(1162..2550)
                     /gene="pntB"
                     /locus_tag="N7B39_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HGU2424362.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(2561..4093)
                     /gene="pntA"
                     /locus_tag="N7B39_004524"
     CDS             complement(2561..4093)
                     /gene="pntA"
                     /locus_tag="N7B39_004524"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HGU2424363.1"
                     /translation="MRIGIPRERLTNEPRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGCFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKNGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQVAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
BASE COUNT         1076 a         1204 c         1062 g         1109 t
ORIGIN      
        1 caaaatattt tcacttcttt acctgagcgg tttgattttc gttatgatga cggagcgaaa
       61 aagacattat tattagcaaa ggaagaaaaa acggggacaa gcatggcaaa gccgctcatc
      121 acgctcaatg gcctaaaaat cgtcattatg ttgggaatgc tggtcattat tctctgcggt
      181 atccgttttg ccgccgagat catcgtgccg tttattctcg cattatttat tgctgttatt
      241 cttaacccgc tggtgcaaca catggtccgc tggcgtgtgc cgcgtgtact ggcagtgtcg
      301 attttgatga ccatcatcgt gatggctatg gtgttgctgt tagcttatct gggttccacg
      361 ctcaacgagt tgacgcggac gttaccgcaa tatcgcaact ctattatgac gccgctgcaa
      421 gctcttgagc cgttgttgca acgcgtaggg attgacgtct cagttgacca actggcgcat
      481 tacattgatc cgaacgcggc gatgacgttg ctcaccaact tattgacgca gttatctaat
      541 gccatgtcat caatattttt attgctgctg acggtgctgt ttatgctgct cgaagtgcca
      601 caattgcccg gaaaatttaa gcaaatgatg gcgcgtccgg ttgaagggat ggcggcgatt
      661 cagcgtgcga ttgacagtgt gtctcattat ctggtgctga aaacagccat cagcatcatc
      721 accggcctgg tcgcatgggc gatgctcgcc gccctcgatg ttcgcttcgc ttttgtctgg
      781 ggattgctgg cctttgcgct taattacatc cctaatattg gttcagttct cgcggcaatc
      841 ccccctattg ctcaggtact ggtgtttaat ggcttctacg aagcgttgtt ggtgctggcg
      901 ggatatctgc tgattaatct ggtcttcggc aatattctgg agccacgcat catggggcgt
      961 gggctgggac tttccacatt ggtggtattt ttgtcgttga ttttttgggg atggttgtta
     1021 ggaccggtgg gtatgctgct ttccgtgccg ttaacaatta ttgtcaaaat tgcgcttgaa
     1081 caaacagcgg gaggtcaaag catcgccgtt ctgttaagcg atctcaataa agagtgacgg
     1141 cctccgcaga ggccgtcagg gttacagagc tttcaggatt gcatccacgc tggctttggc
     1201 gtcaccaaac agcatgtggg tgttttcctt gaagaacagc gggttttgca caccagcata
     1261 gccagtgttc atcgaacgtt taaagacaat cacgttctgc gctttccaca cttccagcac
     1321 aggcatacca gcaatcggac tcttcggatc atcctgcgcc gccgggttaa ccgtatcgtt
     1381 agcaccaatc accagtacgg tatcggtatc agcaaagtca tcgttgatct cgtccatttc
     1441 cagcacgatg tcatacggta cttttgcttc agccagcaat acgttcatat gtccaggcaa
     1501 acgccccgca accgggtgga taccgaaacg cacgttgata ccacgagcgc gcagtttctc
     1561 ggtaatttca gcgacaggat attgcgcctg cgcgactgcc atgccgtacc ccggagtaat
     1621 gatcactgaa tgggagtttt tcaacagttc cgctgtctct tctgcggtga tttcgcggtg
     1681 ctcacccact tcctgatcat cgccagtaga agagccgtcg gtgccgaaac cacccgcaat
     1741 aacgctgata aaggaacggt tcatcgcctt acacataatg taagaaagga tagcccccga
     1801 ggaaccgacc agcgcaccgg tcacaatcag caggtcgttg ctgagcataa agcccgcagc
     1861 cgcagccgcc cagccggagt acgagttcag catcgacacc accaccggca tatctgcacc
     1921 accgatggag gcgactaaat gccagccaaa taccagcgca atggcggtca ttattagcaa
     1981 tgccagcact tgcaggccga cgctatccgt gcgaacaaat acaatcagca gcaggaagga
     2041 aacgaccaga gctgccaggt tcattttgtg acggtttggc agcatcaacg gtttagacga
     2101 aattttgcca cacagtttgc cgaacgccac caccgaaccc gtgaacgtta ccgccccgat
     2161 gaagataccg agaaacactt ccgtcaggtg aatattgacc agaatcggtg ccattcccgc
     2221 gtcatgatgc agatagctgt taaagccaac cagcactgcc gccagaccca cgaagctatg
     2281 caggatcgcc accagttctg gcatttcggt catttcaact ttcttcgcca gacggatacc
     2341 aattgcccca ccaatgacca tcgccagcaa gatccagcca acgttacccg tatccggtcc
     2401 aaaaatggtg gcgattagcg caatcgccat cccggcgata ccgaagttgt taccctggcg
     2461 agacgtttca tgtttcgaaa gcccggccag actgaagata aacaggatcg cggcaacaat
     2521 gtatgcagct gtaactaatc ctccagacat atgttacccc ttaatttttg cggaacattt
     2581 tcagcatgcg ctgagtcacg gtgaagccac cgaaaatatt aatgctggct ataagcaccg
     2641 cgataaaact aaggaagcta acccagccgc cctggccaat ctgcaacagt gctccgacaa
     2701 caataatccc tgaaatcgcg ttggtgaccg acatcaacgg tgtatgcagc gcgtgcgata
     2761 cgttccacac cacgtaataa ccgacaacgc aggccagcgc gaaaacggtg aagtgcccaa
     2821 gaaactcttt cggcgcaacg cttgccagcc agccgaaaag gatgattgcc agcgccatca
     2881 acgcgtattt acgccacggt gaacaggcac atttttcctc agttttcact tccggtgccg
     2941 ctttttgtgc cacctgcggc tgagctgata cctgaatcgg cggtgccggc caggtaattt
     3001 cgcccgcacg gatcacagtc acgccgcgaa tcaccacatc atcaaaatca acagtgatgt
     3061 taccgttttt ctctttgcac aacagtttca gcagattaac gaggttagtg ccgtaaagct
     3121 gtgaggattg cgtcggcaga cggcccggaa gatcggtata gccaatcact ttgacaccat
     3181 tttccgtagt gaagatttca cccggcacgg tgtattcaca gttgccgccg ttttgggctg
     3241 ccaggtcgac aatcacactg cccgccttca tggagtcaac catttcacgg gtaattagct
     3301 tcggcgctgg tttgcctgga ataagcgcgg tggtgacaat gatatcgacc tcttttgcct
     3361 gggcggcaaa gagttccatt tccgctttga tgaacgcgtc cgacatcact ttggcatagc
     3421 catcgccgct gcccgcttcc tctttaaaat ccagctcgag gaattccgcg cccatacttt
     3481 gaacttgttc tttcacttcc gggcgggtgt cgaatgcacg cacaatcgcg ccgagactgt
     3541 ttgctgcgcc aatggcggcc agacctgcaa cacccgcacc aatcaccatc acttttgccg
     3601 gtggcacttt cccggccgca gtaatttgcc cggtaaagaa gcacccaaat tcatgtgccg
     3661 cttcaacaat ggcgcgataa ccggcgatat tcgccatcga gcttagtgcg tccagcgatt
     3721 gtgcgcgtga gatacgcggc acagagtcca tcgccatcac ggtcacgtta cgttccgcaa
     3781 gtttttgcat taattccgga ttctgcgcag gccagataaa actcaccagc gttgtcccag
     3841 gattcagtaa cgcaatttca tcatctaacg gcgcattgac cttcagaatg atctctgact
     3901 gccagacgct attcccttct acaatttcag cgcccgcttg cacaaacgct ttatcgtcaa
     3961 aacttgccag ttgacccgcg ccgctctcta ccgcgacggt aaaccccagt ttcagcagct
     4021 gttccactgt ttttggcgtt gctgcaacac ggggttcatt ggttaaccgt tctcttggta
     4081 tgccaattcg catgatattc ccttccatcg gttttattga tgatggtttg cctgtgtcag
     4141 gagccacaca agccgctcat gtacgagcta aatgttactc cgttaaaata aattagtaac
     4201 aaacgcccta taacgtactg aaaattatgc ctgtgatcta gcgccaaaaa tcagtatttc
     4261 ggcgtgaact cgcaaaatat taacgattca gccgtgatag tgggataaac accttagaac
     4321 gccggataaa gactgataat tgtcttcgac ggtcgggtaa aacgagacat cgccccggca
     4381 cgaatcacta cttaacatta aattaactta tacaattcag ttgcttcagt agtaatgatg
     4441 ctgatacggc t
//
LOCUS       DBFIFI010000280         4415 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.280, whole genome shotgun sequence.
ACCESSION   DBFIFI010000280 DBFIFI010000000
VERSION     DBFIFI010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4415
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.280"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..2157
                     /locus_tag="N7B39_004525"
     CDS             <1..2157
                     /locus_tag="N7B39_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HGU2424364.1"
                     /translation="YVGRMARGYVSPDLWEEGINAGLLNYSFNGNSINNRSNHNAGKS
                     NYAYLNLQSGINIGSWRLRDNSTWSYNSGSSNSSDSNKWQHINTSAERDIIPLRSRLT
                     VGDSYTDGDIFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIARGTAQVSVKQNGYDV
                     YQTTVPPGPFTIDDINSAANGGDLQVTIKEADGSIQTLYVPYSSVPVLQRAGYTRYAL
                     AMGEYRSGNNLQSSPRFIQGSLMHGLEGNWTPYGGMQIAEDYQAFNLGIGKDLGLFGA
                     FSFDITQANTTLADGTRHSGQSVKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAY
                     SRMSGYTVKPPTGDSNEQTQFIDYFNLFYSKRGQEQISISQQLGNYGATFFSASRQSY
                     WNTSRSDQQISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDS
                     QSAFRNSNASYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTHGGNTSSGTSGY
                     STLNYRGAYGNTNVGYSRSGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKAPGA
                     DNVKIENQTGIHTDWRGYAILPFATEYRENRVALNANSLADNVELDETVVTVIPTHGA
                     IARATFNAQIGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAENGQVYLTGLPQSGK
                     LQVSWGNDKNSNCIVDYKLPEVSPGTLLNQQTAICR"
     gene            2171..2701
                     /locus_tag="N7B39_004526"
     CDS             2171..2701
                     /locus_tag="N7B39_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2424365.1"
                     /translation="MKYNNIIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDGNMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            2714..3217
                     /locus_tag="N7B39_004527"
     CDS             2714..3217
                     /locus_tag="N7B39_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2424366.1"
                     /translation="MKRLHKRFLLATFCALLTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPAETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDATLKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            3277..4191
                     /locus_tag="N7B39_004528"
     CDS             3277..4191
                     /locus_tag="N7B39_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2424367.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVNLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGTTTDSARQVFANTAPDATKASGVGVSLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
BASE COUNT         1275 a          929 c         1002 g         1209 t
ORIGIN      
        1 tatgttggca gaatggctcg cggctatgtt tctcctgatc tgtgggaaga aggaataaat
       61 gctgggcttc taaactatag ttttaatggt aattctatta ataatcgtag taaccataat
      121 gcaggaaaat ccaactatgc atatttgaat ttacagagtg gcatcaacat tggtagttgg
      181 cgactacgcg ataactcaac gtggagttat aacagtggga gtagcaattc atctgacagc
      241 aataaatggc agcatatcaa tacgtcggct gaacgtgaca ttattccctt acgctcacgg
      301 ttaacggtag gtgatagtta taccgatggc gatatttttg atagtgtgaa ctttcgtggc
      361 ctcaaaataa attcaacaga agcgatgttg cccgatagcc aacatggttt cgctccggtg
      421 attcatggta ttgcccgtgg caccgcacaa gtgagtgtaa aacagaacgg atacgatgtt
      481 tatcagacta ctgttccacc cggccctttt actattgatg atatcaactc tgcggccaat
      541 ggtggtgatc tgcaagtaac cataaaagag gcagacggca gtattcagac attatatgtt
      601 ccttattcgt ctgttccggt tctccaacgt gctggatata cgcgttatgc gcttgccatg
      661 ggggaatatc gtagtggaaa taacctgcaa agctccccca ggttcataca aggtagcttg
      721 atgcatggac tggaaggaaa ctggacacct tatggcggaa tgcaaattgc agaagattat
      781 caggccttca accttggtat tggtaaagat ttaggacttt ttggtgcctt ttctttcgat
      841 atcacgcagg ccaatacgac acttgcagat ggcacccgtc acagcgggca atcggttaaa
      901 tccgtctaca gcaaatcctt ctaccagaca ggaaccaata tccaggtcgc aggatatcgc
      961 tattctacgc aaggttttta taacttatcc gacagtgcct acagtcgaat gagtggttac
     1021 accgtcaagc ctcctaccgg agacagcaat gagcagacac aatttattga ttattttaat
     1081 ctgttctaca gtaagcgtgg tcaggaacaa ataagcatct ctcagcagct tggaaattac
     1141 ggtgcgacat ttttcagtgc cagtcgccaa agttactgga acacgtcacg cagcgaccag
     1201 caaatatcat ttggattaaa tgtgccgttt ggtgatatta cgacttcgct gaattacagc
     1261 tattccaata atatatggca aaacgatcgg gatcatttac tcgcttttac gcttaatgtt
     1321 cccttcagtc attggatgcg tacagacagt cagtcggcat ttcgtaattc aaacgccagt
     1381 tacagtatgt caaacgattt gaaaggcggc atgaccaatc tatcgggggt ttatggcact
     1441 ctgctgccgg ataataacct gaattatagc gttcaggtcg gtaacaccca cggaggtaat
     1501 acatcgtctg gcaccagtgg ttacagtact cttaattatc gtggagctta cggcaatact
     1561 aatgtcggtt acagtcggag tggtgacagc agccagattt attacggaat gagtggtggg
     1621 attattgctc atgctgatgg catcaccttt ggacagccgc tgggcgacac aatggttctg
     1681 gttaaggctc ctggcgctga taatgtcaaa atagagaacc agaccggaat tcataccgac
     1741 tggcgtggct atgccatatt accatttgcg acagaatata gagaaaatcg tgtcgctctt
     1801 aacgcgaatt cccttgcaga taatgttgaa ctggatgaaa ccgtggtcac tgtcatccca
     1861 actcacggtg ctattgccag agcaacattt aatgcacaaa tcggcgggaa agtattaatg
     1921 acgttgaagt acggtaataa aagcgttcca ttcggtgcaa ttgtcactca cggagagaat
     1981 aaaaatggca gcattgtcgc ggaaaacggt caggtttatc tgactggact tccacagtca
     2041 gggaaattac aggtttcatg gggcaatgat aaaaactcaa actgtattgt cgattacaag
     2101 cttcctgaag tctctcctgg aaccttgctg aaccagcaga cagcaatctg tcgctaagga
     2161 acaatagatt atgaaataca ataacattat tttcctcggt ttatgtctgg ggttaaccac
     2221 ctattctgct ttatccgcag atagcgttat taaaattagc gggcgcgtcc tcgattatgg
     2281 ctgcacagtc tcatcggatt cgcttaattt taccgtagat ctccaaaaaa acagtgccag
     2341 acaatttcca acgaccggta gcacaagtcc agccgtccct tttcagatta cgttaagtga
     2401 atgcagcaaa gggacaacgg gggttcgggt tgcatttaac ggtattgagg acgcagaaaa
     2461 taatactctg ttgaaactgg atgagggaag caatacggcc tccggtttag gtatagaaat
     2521 actggacgga aatatgcgtc cggtgaaact gaatgacctt catgccggga tgcagtggat
     2581 cccactggta ccagaacaga acaatatttt gccttactcc gctcgtctga agtcaactca
     2641 gaagtccgtc aatccgggac tggtgagggc ttcggcaacc tttacccttg aatttcaata
     2701 atcagagtca gatatgaaaa gacttcacaa gaggttcctc ttagctacgt tttgcgcgtt
     2761 attaacagca actctccagg ccgccgatgt cactatcact gttaatggtc gggtagtcgc
     2821 taaaccctgc actattcaaa ccaaagaagc taacgttaat ctcggggatc tttatacgcg
     2881 caatctgcaa caacctggtt ctgcatctgg ctggcacaat attactttgt cattaaccga
     2941 ttgtccggct gaaacaagtg cagtgacggc aatcgtgaca ggttcaactg acaatacggg
     3001 ttattacaaa aatgaaggta ctgccgaaaa tattcagata gagcttaggg atgaccagga
     3061 tgcgacgtta aaaaatggcg atagcaaaac ggttattgtt gatgagatca ctcgtaatgc
     3121 acagtttcca cttaaggcaa gagctatcac ggtgaatgga aacgcaagcc agggaacgat
     3181 cgaggcgcta atcaatgtga tctacacctg gcaataacgt cgattcgatt ttcttagcct
     3241 tctttttaaa tcaatttttt aaccttagga tatatcatgg gtaaaacaat cagtataaag
     3301 gtcctgttcg gtatttatct tctattaatg gcaggcaaag tttttgcctt cagttgtaat
     3361 gtcgatggcg ggtcaagtat tggagctggt acaacctctg tttatgttaa tctcgaccct
     3421 gtaatacagc cgggccagaa tctggttgta gacttgtctc agcatataag ttgctggaat
     3481 gattacggcg gctggtacga cactgatcat ataaacctgg tacaaggctc agctttcgca
     3541 ggctctctac agtcatataa aggatcgctg tactggaata atgtgaccta ccccttcccg
     3601 ttgacgacaa ataccaacgt attggatatt ggtgataaga caccgatgcc attaccgtta
     3661 aaactctata ttaccccagt cggggcggcc ggtggagttg tcataaaagc cggggaagtt
     3721 attgcccgta ttcatatgta taaaattgcc acgttaggaa gcggaaatcc gcgtaatttt
     3781 acgtggaata ttatttcaaa taacagtgtt gttatgccaa caggcggctg cactgttgat
     3841 agtcgtaatg tcaccgtgaa tctcccggac tttcctggaa gcgcagaaat cccccttggc
     3901 gtatattgct caagcgaaca aaagctctca ttttatcttt cgggtactac cactgacagt
     3961 gctagacaag tgtttgctaa cacagcacca gatgcaacaa aagcatcggg agtgggtgtc
     4021 tctctgatgc gtaatggaaa aatactggct acgggagaaa atgtctccct ggggacagta
     4081 aataaaagca aagtacctct gggtttaagc gcaacctatg gacaaacagg gaacaaagtt
     4141 tcagccggaa ccgtccagtc agtcattggt gtcactttta tctatgaata attgtgattt
     4201 tgaccttagt ctatgcgctc cattcacgaa aattgcgcgg ataattattt tgtgaaggct
     4261 attatcctac acttgtaaga aaatccgcgc acaattgaag ccagagtctt attttttata
     4321 ttgaaaaata tgttaattta aacactatga gagatgatgc ttaccttatc gtatttaatc
     4381 tattgtttaa cgataataga ttaaaaatag aaaga
//
LOCUS       DBFIFI010000281         4392 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.281, whole genome shotgun sequence.
ACCESSION   DBFIFI010000281 DBFIFI010000000
VERSION     DBFIFI010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.281"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..793
                     /gene="yahL"
                     /locus_tag="N7B39_004529"
     CDS             <1..793
                     /gene="yahL"
                     /locus_tag="N7B39_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HGU2424368.1"
                     /translation="NNLMPYMQQSILNTVKNNQLPEDIKSSLVSCVDIFKVLIKQYYD
                     YPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMDYPDHPVKF
                     VFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFINSVRTDCKCILLEHH
                     MPLLRLFPKGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYPVFPESLVM
                     LLLSVLDVKTYRDDARLNEGISNRVQELGDRYYPVNKHVKIRYTL"
     gene            1245..1454
                     /locus_tag="N7B39_004530"
     CDS             1245..1454
                     /locus_tag="N7B39_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424369.1"
                     /translation="MPLLSSLQSGTLPLFGYSGRVRPMKKAHTGESGLKWEAKDSSKL
                     IGNKDHALRGLSSPMVGIRQIRLIT"
     gene            complement(1471..2142)
                     /gene="yahN"
                     /locus_tag="N7B39_004531"
     CDS             complement(1471..2142)
                     /gene="yahN"
                     /locus_tag="N7B39_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HGU2424370.1"
                     /translation="MMQLVHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPINAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASIIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            2289..2564
                     /gene="yahO"
                     /locus_tag="N7B39_004532"
     CDS             2289..2564
                     /gene="yahO"
                     /locus_tag="N7B39_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein YahO"
                     /protein_id="HGU2424371.1"
                     /translation="MKIISKMLVGALAFAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTADIYKKK"
     gene            complement(2662..4248)
                     /gene="prpR"
                     /locus_tag="N7B39_004533"
     CDS             complement(2662..4248)
                     /gene="prpR"
                     /locus_tag="N7B39_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HGU2424372.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMRQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRQGLQASETVLVHYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARESAKIPAPRLLTPQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
BASE COUNT         1212 a         1032 c         1036 g         1112 t
ORIGIN      
        1 caataatttg atgccatata tgcaacaaag catacttaat acggttaaaa acaatcagtt
       61 accagaggat atcaaaagct ccctggtttc ctgtgtcgat atattcaagg ttttgattaa
      121 acaatattat gattatcctt atgattgtcg tgatgattta gtcgatgacg acaaactcat
      181 tcatctcatg gctgccgtac gagactgtga gtggtcagac gataacgcac tcaccataaa
      241 tgtgcagttt aatgatttcc ctggatttta tgactggatg gattaccctg atcacccggt
      301 taagtttgtt tttcgcatac ttgaaaatca aaaagggaca gtttgggtat atgaccagga
      361 tgatgcgttt cttgatatta aagcaaatgt gcaggctgga cgctttaccg ggcttaagaa
      421 attagtgcag tttatcaatt cagtgcgaac agattgcaaa tgtattcttc ttgaacacca
      481 tatgcctttg ctgagacttt tccctaaagg gaaagagtgt atgcatgtgg aaaaatggct
      541 tcgcgaaatg tcgtctattc cggagacaga cgcgcccatt aagcaggcgc ttgcgcatgg
      601 attattatta cacttaaaaa atatctaccc tgtttttcct gaatcactgg tgatgttatt
      661 gctctctgtt ctggacgtaa aaacatacag agatgatgca cgattaaatg aagggatatc
      721 taatagagtc caggaattag gtgacagata ttatcctgta aataagcacg ttaaaatccg
      781 ctacactctt tagttcagaa agggttccac ctgcaagttg ttattctacc tgagagtgaa
      841 tcgcaatgca ggtaatcatt tcatctgtat atctgtatgc caactggcat tccatctctt
      901 ttttcgccga tattttgttg tgatcggcga cacttcgctt aaaaaagcac cagtagtggt
      961 ttcgcagcca tgcggtgtat aaaaaaaaat gatctcatgc agatgttttg tgaatgtgtg
     1021 ggttgacatt catatgaaaa aaatcataat tccatcatgt ttgtgtgggg tcttttctgt
     1081 atcttacgca tcgcactcaa gcctgacaga aaatatgctg taaggctcat atcaaaaacc
     1141 gccattagct catcaggaag agcagacgac aaccaggatt gttgtatggt acggggttcg
     1201 aggccccgat ggcggtccaa cttttcaaaa ctcttctgaa ttagatcccc ttattatcat
     1261 ccctgcaaag tggaacattg ccattgtttg gctattcagg acgggtcaga ccaatgaaaa
     1321 aagcccacac aggggagagt gggctgaaat gggaagctaa agactcaagt aaacttatcg
     1381 gaaataagga ccacgcatta cgggggctat catcgccgat ggtcgggatt cggcaaatca
     1441 ggttgattac gtagagagta aattatctgc tcaccgctgc gtcacccctt cgtaaatcag
     1501 gcgtagcgcg aatacaccaa taattgcacc aataacccga ctggcaacgc gttgcatacg
     1561 cccataagca cgacgcacag cgggcaaaga aaacgcctga ctaagaaaaa ctcgccagat
     1621 aattgatgcg agcacaatcc ccgcccaggc cattaaacgt gcccatgttg gtgtttcggc
     1681 atttaatgtt actgagaaaa tactaataaa aaataaaacg gtttgtgggt tagagagatc
     1741 ggtaattaat ccacggcgaa aaaagacata ccagggggcg ttaatcggtt gttgtagtgt
     1801 gctcatttgc ggtgttgact ggcggcgcat gctgcaccac gcaaaccata agagataagc
     1861 gccgccgacg attctgataa gcgaaaaaat ctcctcacac tgcgtaatta gcgttgcaag
     1921 accaaacaaa cccaacccgg aataaaatgc atcgcccagc gccacgccca gtccggtcag
     1981 cactcctgcg cgtcgaccgg aagccaggct ggtttgtact accacaaaaa gattggctcc
     2041 cggattaaaa aaagtaatca cgaacagtcc tacggtcagg taaacggcat gcaaaggatc
     2101 catagtgatt tcatccataa ataagtgaac taactgcatc atatttctac caaaaataat
     2161 cgggtgcgag agagatcaca aagtgtctta tttccggtta ctggcgttta tgccctgact
     2221 gaactaatta ttaatcaacc caataatgtc ggtgggtgat agtgtgataa caactctgga
     2281 gccgtaatat gaaaataatc tctaaaatgt tagtcggtgc gttagcgttt gccgttacca
     2341 atgtctatgc cgctgaattg atgaccaaag cggaatttga aaaagttgaa tcgcagtatg
     2401 aaaaaatagg tgatatttca accagtaatg aaatgtcgac tgcagatgca aaagaagatt
     2461 taatcaaaaa agccgatgaa aaaggggctg atgtgttggt actgacctcc ggtcaaactg
     2521 acaataagat ccacggcacg gcggatattt ataagaagaa gtaattctga atcctatgta
     2581 aacatccccg atgcgtaagt ttatcggtga tcatctattg aaatttatgc cggataaagc
     2641 gttcgcgctg cattcggcag ttcagctttt cagccgccgc cagaacgtcg tccggctgat
     2701 gcctaaataa ttcgccgctg ctgttttatc gccattaaat ttctccagtg cctgttgtgg
     2761 tgtcagcaag cgtggagcgg gaattttcgc cgactcgcgc gccagttccg gcagtagcag
     2821 ctgcaaaaac tgcggcgtta aatccggcgt cggttccaca cttaaaaata gcgccagtcg
     2881 ctccatcata ttgcgcagtt cacgaatatt acctggccag tcgtagtgca ccagcacggt
     2941 ttcgcttgcc tgtaatccct ggcgtaatgc ggcagaaaat ggggcggaga gcgccgccag
     3001 agacactttc aaaaagcttt ccgccagcgg aagaatatct gccacccgct cgcgcagtgg
     3061 tggcaattgc agacgcaaaa tactcagccg ataaaacaga tcacggcgaa actgcccttg
     3121 ccgcatatct tcttccagat tgcagtgagt ggcgctaatg acccgtacat ctaccggaac
     3181 aggctgatgc ccgccgacgc gggtgacctc tttttcttcc agcacccgta acaggcgagt
     3241 ctgcaaaggt agcggcattt cgccaatctc atccagaaac agcgtgccac cgtgggcaat
     3301 ttcgaacagc ccggcgcgac ctccgcgtcg cgagccggta aacgcccctt cctcatagcc
     3361 aaacagctcc gcttccagca gcgattcggc aatcgccccg cagttgacgg caacaaacgg
     3421 atgcgacttt ttgccctgtc gcgcatcgtg gcgggcaaaa tattcccgat gaattgcctg
     3481 ggccgccagc tctttgcccg tccccgtttc cccctcaatc aacaccgccg cactggagcg
     3541 ggcatacagc aaaatagtct gccgcacctg ctccatctgt ggtgattgac cgagcatatc
     3601 gcccagcacg taacgagtac gcagggcgtt gcgggtggca tcgtgagtgt tatggcgtaa
     3661 cgacatgcgc gtcatatcca gcgcatcgct gaacgcctgg cgcacggtgg cggcggaata
     3721 gataaaaatt ccggtcattc cggcttcttc tgccagatcg gtaatcagcc ccgcgccgac
     3781 caccgcttcg gtgccgttag cttttagctc gttaatctgc ccgcgtgcgt cttcttcggt
     3841 aatgtagcta cgttgatcga ggcgcaaatt aaaggttttt tgaaacgcca ccagtgccgg
     3901 aatagtttcc tgataagtga caacgccgat agaagaggtg agttttccgg cttttgccag
     3961 tgcctgtaac acatcgtagc cgctcggttt aatcaaaata actggcactg acaggcggct
     4021 tttcaggtac gcgccgttag agccagcggc gatgatggcg tcacagcgtt cgtttgccag
     4081 tttcttgcgg atgtaggtca ctgctttttc aaagccaagc tgaatagggg taatgttcgc
     4141 caggtgatca aactcgaggc tgatatcgcg aaacagctcg aataagcgcg ttacagatac
     4201 cgtccagata accggtttgt cgtcattcag ccgtggtgga tgtgccatag cgcaccgcaa
     4261 agttaagata ccgaatattg ggtttagtct tgtttcataa ttgttgcaat gaaacgcggt
     4321 gaaacattgc ctgaaacgtt aactgaaacg catatttgcg gattagttca tgactttatc
     4381 tctaacaaat tg
//
LOCUS       DBFIFI010000282         4275 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.282, whole genome shotgun sequence.
ACCESSION   DBFIFI010000282 DBFIFI010000000
VERSION     DBFIFI010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4275
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.282"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            1..97
                     /locus_tag="N7B39_004534"
                     /pseudo
     CDS             1..97
                     /locus_tag="N7B39_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309987.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(202..272)
                     /locus_tag="N7B39_004535"
     tRNA            complement(202..272)
                     /locus_tag="N7B39_004535"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(242..244),aa:Met,seq:cat)
     gene            complement(436..993)
                     /locus_tag="N7B39_004536"
     CDS             complement(436..993)
                     /locus_tag="N7B39_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000211421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HGU2424373.1"
                     /translation="MTTQISVETLSPITHNQIPVITTELLAQLYCTEINNIKVNYTRN
                     SERFVEGKHFFKVVGDELKNLRVTLSNSQTPVSPKTRSLILWTERGAARHAKMLETDQ
                     AWDVFEKLEDCYFRQKDPSAPASCQKSYDTRVLCYQRGGVTVSTIQLRDDDIVISLES
                     WLELARANGWFVVRRDKLVERLMQL"
     gene            complement(1267..1821)
                     /locus_tag="N7B39_004537"
     CDS             complement(1267..1821)
                     /locus_tag="N7B39_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HGU2424374.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLTSKKLTKTAINEALRRMKKAGLDKPELEAFLRDMINGKQKSWLVHCTDAEAL
                     CIDRVISEVLAEHPGLICILRQRYEGRGMTKRKMAELLNDSHPEWCYATCRNRIDAWL
                     KMAEFMLYLPMRDAFSSGDLKTVC"
     gene            complement(1818..2108)
                     /locus_tag="N7B39_004538"
     CDS             complement(1818..2108)
                     /locus_tag="N7B39_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HGU2424375.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(2108..2707)
                     /locus_tag="N7B39_004539"
     CDS             complement(2108..2707)
                     /locus_tag="N7B39_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HGU2424376.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKRVSGFVDYLCESVGREHTPALSEAAEQ
                     YLNTVATRRTRDTALLKSFEAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEEVENVAAQLLEFA"
     gene            complement(2863..2940)
                     /locus_tag="N7B39_004540"
                     /pseudo
     CDS             complement(2863..2940)
                     /locus_tag="N7B39_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3161..3316)
                     /gene="hokD"
                     /locus_tag="N7B39_004541"
     CDS             complement(3161..3316)
                     /gene="hokD"
                     /locus_tag="N7B39_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HGU2424377.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVTVF
                     TAYEPEE"
     gene            complement(3929..>4275)
                     /locus_tag="N7B39_004542"
     CDS             complement(3929..>4275)
                     /locus_tag="N7B39_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424378.1"
                     /translation="EQLIAHAEETIEAQRLCIPGTIDHDIIRTYKMDIAVLEIALASL
                     AAEPAGKLHEYKPVGYQRLVDELTMQVKQLAWQLKKAKPDCKLPDKAMDYLKRNGLIS
                     VEDALRRVSKNM"
BASE COUNT         1085 a         1110 c          924 g         1156 t
ORIGIN      
        1 gtgcctcccg gtgaattcag taccagcacc tgaatccgcg attatcccat atacctactc
       61 actgattgcc cctccgcaca gggggattca ccatgccagt ttcttttaac aaactccccg
      121 caaaccagac aacagtcaac cgcctgaatt gtgagacatt taaaaaaaag gcccgcaaaa
      181 gcgagccagg gaaaataagt gtggcgcgtt gtactggatt cgaaccagcg acctggcgat
      241 tatgcgtcgc tcgctctcac cactgagcta aagggccggg agcagaataa taacggtccg
      301 taattaattc cgcaataaaa aacccgctca atggcgggtt ctggtaaagt tcatgcgctt
      361 ggttcgcctc gcgatacagc tttgcgaagc gtaccggaat tgaagcagtt tatggctaaa
      421 attgcaagaa cttttttaaa gctgcatcag cctttccacc agtttatctc tgcgaacaac
      481 aaaccaacca ttggctctcg ccagttccag ccatgactca agggaaataa caatatcatc
      541 atcccgcaac tgaattgtgg aaacagtgac accgcctcgc tgataacaga gaactcgcgt
      601 gtcgtaactt ttctggcatg aagctggcgc tgacggatcc ttttgtctga aatagcagtc
      661 ttccagtttt tcgaacacat cccacgcctg atcggtttcg agcattttgg cgtgacgggc
      721 tgcgcctcgt tctgtccaga ggatgaggga gcgggttttg ggggaaacag gcgtttgtga
      781 gttacttaaa gtaacccgca aatttttcaa ctcatcacca acaaccttga aaaagtgttt
      841 accctcaaca aaacgctcgg aattacgggt gtaatttact ttgatgttgt tgatctcggt
      901 acagtaaagc tgtgccagta actcggtagt gataacggga atttggttgt gtgtaattgg
      961 ggaaagagtt tcgacagaaa tctgagtggt cataacgata actccgtaca tttggacatt
     1021 atcgccaccg tcaggtgcta atcatcgtgg tggcgaactg tgcggggtta gcactaccgg
     1081 gtacggaaac cggcgagcct ttcggctccc ccacacagcc cgccataaat cgcgaatgtg
     1141 actgtgcaaa cgatatgaaa aaagacgcgg gcgcgtctca tatcgctccg taaacatccg
     1201 gggtgctaat cccgacgcca gattttgctg gcgcgtgagg aatatagccc cggataacag
     1261 attgagtcaa cagacggttt ttagatcccc ggaagagaat gcatcacgca tcggcagata
     1321 gagcataaac tctgccattt tcaaccacgc atctatgcga ttacggcacg tggcgtaaca
     1381 ccactcaggg tgtgaatcat tcagcaattc agccattttt cgcttagtca tccccctccc
     1441 ttcatatcgt tgccggagga tacaaatcaa tcctgggtgt tctgccagca cttcactaat
     1501 cacacgatca atgcataacg cctctgcatc agtacaatgc accagccagc ttttttgctt
     1561 gccgttgatc atatcccgca aaaaagcctc aagttcaggt ttgtccagac ctgctttttt
     1621 catcctccgg agcgcctcgt taattgccgt ttttgtcagc tttttagagg tcagcaactg
     1681 gttgaacata tttcccgtct taccgccgcc aatatacgac cagcgccccc acatgcgcag
     1741 ttttccctga atccagacac tttccagcgt ggtgagacga aggtgttccc cgcttttgcc
     1801 tgtatttgtt gggtaaatca taaataacct tcctttctcc agatttcttg cgtacgaaaa
     1861 acaccttctg catgcatcag gcgtaattct tctttggtgt aatcgctggt ttttacccgc
     1921 ccgtcgatta aatcgtggca tgagctacag gcaatcgctg cctgcatatc gtgtggtttt
     1981 gtcgctgttc cgcacgtccc cgccagcctg taatgcgcca gcacagaagt ttcgggattg
     2041 tgattgcagt agccaggaat tctgacggtg cacatctgcc cccgcgccgc tttacgtaaa
     2101 tccaccatta cgcaaactcc agcagctgcg cggccacatt ttcgacttcc tccggagagg
     2161 aaaatttacg gaacaggatc cagttccaca gcacattcag tacagattta taaacctgct
     2221 gaaactcggt ttcgtccatg ttcgcaaatg cgatagattt tgccctgcgc ccacgactac
     2281 catcaggata aatatgctcg gtgtaaaatc ctgcctgaat ggttacccac tcgcggaaag
     2341 cctcaaacga ctttagcaat gctgtatctc gggttctgcg tgtcgcaact gtattcagat
     2401 attgctctgc ggcttcgctc agagctggcg tatgttcccg gcctactgat tcgcacagat
     2461 aatcaacgaa accggacacc cgttttcgct cgcgaggagt gatcgcccca ccgaccggag
     2521 tccagtaatc gaatccgagt tgcagaagtt tgaaaaaacg cttatggaat gcgtagttac
     2581 gaacgcgctt gaaatcagcg tgtatccact cgcctatttt gatttgatgc agaaaatcac
     2641 aactctccgg cgtcgccggg agaagtaatc cggaagaggt ttgtttgacc agttgtatat
     2701 gcgccattgc tgtctccaat ggcgctgtag gttgccagtt gttcaggctg gcttacgaat
     2761 tataactcat tcccgaatca ccttgaaacc gagcctttca agatattcaa tgaatgcctc
     2821 gatagataaa attacatgat tatcagggat taacgttgcg tagataactt ccccattctc
     2881 aacgcgcaca gcaaagaggc cattttcact aaaaatttca cgtaattctt caattttcat
     2941 caacagaatc cttccagata aatagcaccc ccctgttcgg ggtccatccc tcttctccct
     3001 gcgcgctact taagtatttt tgattctatt ccggcatcgt ccataacttc aaacgtattg
     3061 aaaataaaaa caaaaacccg ccgaagcggg ttaagtgcgg gtgcgttgaa gatgcctgac
     3121 acatgagagg tggcgaggga tttctccctc gcctggtctc ttactcctca ggttcgtaag
     3181 ctgtgaagac agtgacctcc gtctggccgg ttcggattcg tacctcgcag aggtctttcc
     3241 tcgttaccag tgccgtcaca atgacggtta aacagatgac gatcagggcg attagcatcg
     3301 ccttttgctg cttcatagcc tgcttctcct tgcctttcgg cgcgtaagag gctaacctac
     3361 atgtgcaagt catagatatg gcctcagatt aatgttaagc gtcttgccgg acgcgtaatg
     3421 ttaactgggg cttttctcta tctgcctttg gcggcatgcc cgaggcagat agcctcaagc
     3481 acccgcagca attctactta agttaggtaa gcttttctat ggtatttctc cacgctccat
     3541 aaatacgaac ttgaatcatc atttttatca cagatgtgag gaaaatatat agtggccttc
     3601 atcaagcgta acgaaataca cctcccattc atctccattc ttcaatttta tacgaaggct
     3661 ttctccacga gaatatttat caaacaactg attcgatgtg ctagtaagag gtatttttac
     3721 cgttgtaaca actattgcac taccatccga ttctctcaac tcaacatcca catccacaaa
     3781 agatgaatgt atgttatatt ccggatatcc aacaattatt cgcacattca ctccttaaag
     3841 acagcaatta aaataaacaa aaaaccaaca tatcagcttg ttttataaca tgaggggtgt
     3901 atcaaaggaa ggttcatggc acacctactt acatgttttt gctaacccgc cgtaaagcat
     3961 cctctacgct tatcagtccg tttcgcttca ggtagtccat cgctttatcc ggtaatttgc
     4021 agtccggttt cgctttcttc agttgccagg ctaactgctt tacctgcata gttaactcgt
     4081 cgaccagacg ctgatacccc actggtttgt attcatgcaa tttaccggct ggctctgctg
     4141 ccagcgatgc cagtgcgatt tccagaacag caatatccat cttatatgtg cggatgatgt
     4201 catggtcgat tgtgcccggt atacacagtc tctgtgcttc aatagtctcc tctgcgtgag
     4261 ctattaactg ctctc
//
LOCUS       DBFIFI010000283         4216 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.283, whole genome shotgun sequence.
ACCESSION   DBFIFI010000283 DBFIFI010000000
VERSION     DBFIFI010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4216
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.283"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            227..835
                     /locus_tag="N7B39_004543"
     CDS             227..835
                     /locus_tag="N7B39_004543"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HGU2424379.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVTQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            933..2324
                     /gene="selA"
                     /locus_tag="N7B39_004544"
     CDS             933..2324
                     /gene="selA"
                     /locus_tag="N7B39_004544"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HGU2424380.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2321..4165
                     /gene="selB"
                     /locus_tag="N7B39_004545"
     CDS             2321..4165
                     /gene="selB"
                     /locus_tag="N7B39_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HGU2424381.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALNIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDPPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQCLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
BASE COUNT          972 a         1068 c         1211 g          965 t
ORIGIN      
        1 gctcatcctt gttttatcgg gttatttgtc tgcttccagg caaaacccta cagatttata
       61 agaaagcctt aagtgagata tccatcagat agaaacaatt aggaaaatat ttatatcacc
      121 atgataaata tgtgatttat ttggaagtaa caaaccatca acattttctg ccctttttcc
      181 catcaccagg ccatactgat atttttcctc gcacaaagga ctcggcatga aactcgtcgg
      241 tagctacacc agcccgtttg tacgcaaact ttctattctg ttgttagaaa agggcataac
      301 tttcgaattt attaatgaac tgccctataa cgcggacaac ggcgtgacgc aatttaaccc
      361 gttaggaaaa gtgccggtgc tggtgaccga agagggcgaa tgctggtttg attcgccgat
      421 catcgctgaa tatattgaat taatgaatgt cgctccggcg atgttgccgc gcgatccgct
      481 ggagtcgttg cgggtgcgca aaattgaggc actggcggat ggcattatgg atgccgggct
      541 ggtatcggtg cgtgaacagg cgcgtccagc ggcgcagcag tctgaagatg aattgttacg
      601 ccagcgggag aaaatcaacc gcagtctgga tgtgctggaa ggatatctgg tcgatggcac
      661 actcaaaacc gatacggtca atctggcgac tatcgccatt gcctgtgccg tcggatatct
      721 caatttccgc cgcgttgcgc caggttggtg tgtcgaccgc ccacacttag tcaaactggt
      781 cgaaaacctg tttagccgcg aaagttttgc tcgcaccgaa ccgccaaagg cttgatgcgc
      841 gatatgtcct cctgacccat ctcacgttac aatccgtggt tatgttaaac gcccttctcc
      901 gtgtgagagg gccttgatca gccaggtttc ctatgacaac cgaaacgcgt tccctctata
      961 gtcaacttcc ggctattgat cgcttattgc gcgatagctc cttcctttct ttgcgtgata
     1021 cttatggtca cacccgcgtg gtggaattgt tgcgtcagat gctcgacgaa gcgcgagaag
     1081 tgattcgtgg cagccagacg ctgcctgcgt ggtgtgaaaa ctgggcgcaa gaagtcgatg
     1141 cccggttgac gaaagaagcg cagagcgcgc tgcgtccggt gatcaacctg acgggaaccg
     1201 tgctgcatac caaccttggg cgagctttac aggcggaagc cgcggtggaa gccgttgcgc
     1261 aggctatgcg ttcgccagtg accctcgagt acgatctgga cgacgccgga cgcggacatc
     1321 gcgatcgggc gctggcgcag ctgctgtgcc gtattacggg ggcggaagat gcctgtatcg
     1381 tcaataacaa tgcggcggcg gtgttattga tgttggcggc cactgccagc ggaaaagagg
     1441 tggtggtatc tcgcggcgaa ctggtggaga ttggcggcgc gtttcgtatt cccgatgtta
     1501 tgcgtcaggc aggctgcacc ctacacgaag tagggaccac caaccgcacg cacgcgaatg
     1561 attatcgtca ggcggtgaat gaaaataccg cactgttgat gaaagtacat accagtaact
     1621 acagcattca ggggttcacc aaagcgatag atgaagcgga actggtggcg ctcggcaaag
     1681 agctggatgt tcccgtagtg actgatttag gcagtggctc gctggtcgat cttagccagt
     1741 acggtttgcc gaaagagcca atgccgcagg agttgattgc ggcgggcgtc agtctggtga
     1801 gcttctccgg cgacaagttg ttaggcgggc cgcaggcagg gattatcgtt ggtaaaaaag
     1861 agatgatcgc ccgcctgcaa agccacccgc tgaagcgtgc attacgcgcg gataaaatga
     1921 ccctcgcggc gctggaagcc acgttgcgtc tttatttaca ccctgaagct ctgagtgaaa
     1981 aattaccgac cctgcgcctg cttacccgca gcgcagaggt cattcaaatc caggcacaac
     2041 gtttacaggc tccccttgcc gcacattacg gcgcggagtt tgcggtacag gttatgccat
     2101 gtctttcgca gattggcagt ggttcgctgc cggttgatcg cctgccgagc gcggcattaa
     2161 cgtttacacc ccatgatgga cgcggtagcc accttgagtc attagccgcc cgctggcgtg
     2221 aattgccagt gccggtgatt ggtcgtattt atgacggacg attgtggctg gatttacgct
     2281 gccttgaaga tgagcaacgg tttttggaga tgttgttgaa atgattattg cgactgccgg
     2341 acacgttgac cacggcaaaa caaccttatt gcaggcgatt actggcgtaa atgctgaccg
     2401 tctgccggaa gaaaaaaagc gcggcatgac catcgatctc ggctatgcct actggccgca
     2461 gccggatggt cgcgtgcctg gttttatcga cgtacccggt catgaaaagt ttctttccaa
     2521 catgctggcg ggcgttggtg gtatcgatca cgcgctgttg gtggtggcgt gcgatgacgg
     2581 cgtgatggca cagacccgtg agcatctggc gattttgcag ctgaccggta acccgatgct
     2641 gacagtggcg ctgaccaaag ccgatcgcgt agacgaagcg cgtgttgatg aggttgaacg
     2701 ccaggtaaag gaggttctgc gggaatacgg ttttgctgag gcaaaactgt ttatcaccgc
     2761 agcaaccgaa ggtcggggaa ttgatgccct gcgcgagcat ctgcttcagt tgccggaacg
     2821 cgagcacgcc agccaacata gtttccgcct cgcgattgac cgcgcattta ccgtaaaagg
     2881 tgccgggctg gtcgtcaccg gtacggcgtt aagcggggaa gtgaaggtag gcgattcact
     2941 ctggctgact ggtgtaaata aaccgatgcg tgtacgtgcg ctgcatgcgc aaaaccagcc
     3001 aacagaaacc gccaatgccg ggcagcgtat cgcgcttaac atcgcgggtg atgcggaaaa
     3061 agagcagatt aaccgtggcg actggctgct tgccgatgtg ccgccagagc cgttcacacg
     3121 ggtgattgtc gagcttcaaa cccatacacc gctgacccag tggcagccgc tgcatattca
     3181 ccacgccgcc agccacgtca cgggacgcgt ttcactgctg gaagataacc ttgctgaact
     3241 ggtcttcgac cccccgttat ggctggcaga taacgaccgc ctggtattgc gcgatatctc
     3301 tgcccgcaac acgctggccg gagcgcgcgt cgtgatgctt aacccgccgc gtcgcggtaa
     3361 acgtaaacca gagtatctgc aatgcctggc ttccctcgcc agggcacaga atgatagcga
     3421 agcgttagcc gttcacctgc aacgcggcgc ggttaatctt cctgatttcg cctgggcgcg
     3481 ccagctcaac ggcgaaggga tgcgcgaact gctgcaacag cctggctata ttcaggctgg
     3541 ttatagcttg ttgaatgcgc cggttgccgc tcgctggcaa cggaaaattc tcgacacatt
     3601 agcgacttat catgagcaac atcgcgatga acctgggcct gggcgcgaac gtctgcgacg
     3661 tatggcgttg ccaatggaag atgaagcgct agtactgttg ctgattgaaa agatgcgcga
     3721 aagcggcgac atcctcagtc atcacggctg gctgcatctg ccggatcaca aagcgggctt
     3781 cagcgaagag cagcaggcca tctggcaaaa agcagagccg ctgtttggtg acgagccgtg
     3841 gtgggtgcgt gatctggcaa aagagacggg taccgacgag caggcaatgc gcctgacgct
     3901 acgccaggcg gcgcagcaag gaataattac cgcgatcgtc aaagatcgtt attaccgtaa
     3961 cgatcggatt gtcgaatttg ccaatatgat ccgcgatctc gatcaggagt gtggttcaac
     4021 ctgcgcggcg gatttccgcg atcgcttagg cgtaggccga aagctggcaa ttcagattct
     4081 ggaatatttt gaccgcattg gctttacgcg tcgtcgtgga aatgatcatt tattgcgcga
     4141 cgcattatta tttccggaaa aataaggaaa tgattaatta atttttaaaa taaattaata
     4201 caaatttctt atgatt
//
LOCUS       DBFIFI010000284         4137 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.284, whole genome shotgun sequence.
ACCESSION   DBFIFI010000284 DBFIFI010000000
VERSION     DBFIFI010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4137
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.284"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            172..1275
                     /gene="proB"
                     /locus_tag="N7B39_004546"
     CDS             172..1275
                     /gene="proB"
                     /locus_tag="N7B39_004546"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HGU2424382.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1287..2540
                     /gene="proA"
                     /locus_tag="N7B39_004547"
     CDS             1287..2540
                     /gene="proA"
                     /locus_tag="N7B39_004547"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HGU2424383.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVVVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIAYSFLPALSKQ
                     MAESGVTLHADASALAQLQTGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2655..2730
                     /locus_tag="N7B39_004548"
     tRNA            2655..2730
                     /locus_tag="N7B39_004548"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2688..2690,aa:Thr,seq:cgt)
     gene            complement(2745..3665)
                     /locus_tag="N7B39_004549"
                     /pseudo
     CDS             complement(2745..3665)
                     /locus_tag="N7B39_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(3610..3855)
                     /locus_tag="N7B39_004550"
     CDS             complement(3610..3855)
                     /locus_tag="N7B39_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein N"
                     /protein_id="HGU2424384.1"
                     /translation="MCDTKRSIGRKCESGLAANVPLRGVFVQDYDSHTQPKLTNRRIQ
                     MDAQTRRRERRAEKQAQWKADRILASTFRRNATKRHY"
BASE COUNT          943 a         1023 c         1097 g         1074 t
ORIGIN      
        1 aatgtggtaa tttattaaat ctgtaataaa agcgtaaaca actgccgcta ggcttgctga
       61 tcccgcgcaa caaaacgcca tgctttgctc gcagatggtt ggcaaccgac gacagtcctg
      121 ctaaaacgtt cgtttgatat catttttcct aaaattgaat ggcagagaat catgagtgac
      181 agccagacgc tggtggtaaa actcggcacc agtgtgctaa caggcggatc gcgccgcctg
      241 aaccgtgccc atatcgttga acttgttcgc cagtgcgcgc agttacatgc cgccgggcat
      301 cggattgtta ttgtgacgtc gggcgcgatc gccgccggac gtgagcacct gggttacccg
      361 gaactgccag cgactatcgc ctcgaaacaa ctgctggcgg cggtagggca gagtcgactg
      421 attcaactgt gggaacagct gttttcgatt tatggcattc acgtcgggca aatgctgctg
      481 actcgtgctg atatggaaga ccgtgaacgc ttcctgaacg cccgcgacac cctgcgtgcg
      541 ttgctcgata acaatatcgt tccggtaatc aatgagaacg atgctgtcgc tacggcagag
      601 attaaagtcg gcgataacga caacctttct gcactggcgg cgattctggc gggtgccgat
      661 aaactgttgt tactgaccga tcaaaaaggt ttgtataccg ctgacccgcg cagcaatccg
      721 caggcagaac tgattaaaga tgtttacggc attgatgacg cactgcgcgc gattgctggt
      781 gacagcgttt caggcctcgg aactggcggc atgagtacca aattgcaggc cgctgacgtg
      841 gcttgccgtg cgggtatcga caccattatt gccgcgggca gcaagccggg cgttattggt
      901 gatgtgatgg aaggcatttc cgtcggtacg ctgttccatg cccaggcgac tccgcttgaa
      961 aaccgtaaac gctggatttt cggtgcgccg cctgcgggtg aaatcacggt agatgaaggg
     1021 gcaaccgccg ccattcttga acgcggcagc tccctgttgc cgaaaggcat taaaagcgtg
     1081 actggcaact tctcgcgtgg tgaagtcatc cgcatttgta acctcgaagg tcgcgatatc
     1141 gcccacggcg tcagtcgtta caacagcgat gcattacgcc gtattgccgg acaccactcg
     1201 caagaaattg atgcaatact gggatatgaa tacggcccgg ttgccgttca ccgtgatgac
     1261 atgatcaccc gttaaggagc aggctgatgc tggaacaaat gggcattgcc gcgaagcaag
     1321 cctcgtataa attagcgcaa ctctccagcc gcgaaaaaaa tcgcgtgctg gaaaaaatcg
     1381 ccgatgaact ggaagcacaa agcgaaatca tcctcaacgc taacgcccag gatgttgctg
     1441 acgcgcgagc caatggcctt agcgaagcga tgcttgaccg tctggcactg acgcccgcac
     1501 ggctgaaagg cattgccgac gatgtacgtc aggtgtgcaa cctcgccgat ccagtggggc
     1561 aggtaatcga tggcggcgta ctggacagcg gcctgcgtct tgagcgtcgt cgcgtaccgc
     1621 tgggggttat tggcgtgatt tatgaagcgc gcccgaacgt gacggttgat gtcgcttcgc
     1681 tgtgcctgaa aaccggtaac gcggtgatcc tgcgtggtgg caaagaaacc tgtcgcacta
     1741 acgcggcaac ggtggtggtg attcaggacg ccctgaaatc ctgtggctta ccggcgggtg
     1801 ccgtgcaggc gattgataat cctgaccgtg cgctggtcag tgaaatgctg cgtatggata
     1861 aatacatcga catgctgatc ccgcgcggcg gggctggttt gcataaactg tgccgcgagc
     1921 agtcgacgat cccggtgatc acaggtggta taggcgtatg ccatatttat gttgatgaaa
     1981 gtgcagagat tgctgaagcc ctgaaagtaa tcgtcaatgc gaaaactcag cgtccgagca
     2041 catgtaatac ggtagaaacg ttgctggtga ataaaaacat cgcctatagc ttcctgcccg
     2101 cattaagcaa acaaatggcg gaaagcggcg tgacgttaca cgcagatgca tctgcgctgg
     2161 cgcagttgca gacaggccct gcgaaggtgg tggcggttaa agccgaagag tatgacgatg
     2221 agtttctgtc attagatttg aacgtcaaaa tcgtcagcga tcttgacgat gccatcgccc
     2281 atattcgtga acacggcacg caacactccg atgcgatcct gacccgcgat atgcgcaacg
     2341 cccagcgttt tgttaacgaa gtggattcgt ccgctgttta cgttaacgcc tctacgcgtt
     2401 ttaccgacgg cggccagttt ggactgggtg cggaagtggc ggtaagcaca caaaaactcc
     2461 acgcgcgtgg cccaatgggg ctggaagcac tgaccactta caagtggatc ggcattggtg
     2521 attacaccat tcgtgcgtaa ataaaaccgg gtgatgcaaa agtagccgtt tgattcacaa
     2581 ggccattgac gcatcgcccg gttagtttta accttgtcca ccgtgattca cgttcgtgaa
     2641 catgtccttt cagggccgat atagctcagt tggtagagca gcgcattcgt aatgcgaagg
     2701 tcgtaggttc gactcctatt atcggcacca tttaaatcaa taagttacac atcattagta
     2761 ccttccttat tttttgactg ggacaaattt gggaccgatg ggttcaggat cgagtctatt
     2821 tgccgtgcgt gttcggtaag gtgattaggt gcaaggtgag catatcgacg aaccatttcg
     2881 atagactccc agcctcccat ttcctgtaac actgacaacg ggactccggc ttgaaccagc
     2941 caacttgccc aggtgtgtct caagtcgtga aatctgaaat catcaatacc agcccgtctc
     3001 agcgccgctt tccaggctgt gtttgcgtca taccgcatct tccttactgt tggcgctttc
     3061 gttccgtctg gtttggtaca gctttccttg tacacaaata cccaacggtg atgattaccg
     3121 atttgttttt tcaatacgcg acatgcagta tcattcagcg caacgccaat tgcgcggttt
     3181 gatttactct cttccgggtt tatccatgcc acccggcgct gcatatctat ttgttgccat
     3241 tcaaggttga tgatgttcga gcgtcttaag cctgttgcca gtgcaaattc aacaacagac
     3301 tttaatggct ccggacattc atcaatcagc ctttgtgctt catggggctc cagccagcgg
     3361 atccgtttat tctttggttg aggcacttta ataattggtg ccttatcgag cattttccat
     3421 tcacgctctg cggctcttag cagggccttt ataaatgaaa gatgcgtagc cttcgttgca
     3481 acggacgctg gttttggcgt gtattctgga acaggtttcc ctttttttct gcatgcttct
     3541 gccctgagtt tccagttttc ctcatgacgc cggttcgtca ttttctgcat tgctgaataa
     3601 atttttgatt cagtaatgtc tcttagttgc attcctgcga aatgttgaag ccagaatccg
     3661 atctgctttc cattgagcct gtttctctgc gcgacgttcg cggcggcgtg tttgtgcatc
     3721 catctggatt ctcctgttag ttagctttgg ttgtgtgtgg gagtcgtagt cctgaacgaa
     3781 aacaccccgc aatggcacat tggctgctaa tccggattcg cacttccggc caatgcttcg
     3841 tttcgtatca cacacaccaa agccttctgc tttgaatgct gcccttcttc agggcttaat
     3901 ttttaagagc atcaccttca tggtggttag tgcgtcctgc tgatgtgctt agtatcaccg
     3961 ctagtggtat ttgtgtcaac accgccagag ataatttatc accgcagatg gttatctgta
     4021 tgttttttat atagatttat tttttgcagg gttgtgtggc ttgggaggtg atcgagagat
     4081 cagaattgcg ttgtttagtg agttgcacct attaatttta aaataaatac aattggt
//
LOCUS       DBFIFI010000285         4136 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.285, whole genome shotgun sequence.
ACCESSION   DBFIFI010000285 DBFIFI010000000
VERSION     DBFIFI010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.285"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(44..2020)
                     /gene="speA"
                     /locus_tag="N7B39_004551"
     CDS             complement(44..2020)
                     /gene="speA"
                     /locus_tag="N7B39_004551"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HGU2424385.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2029..2160)
                     /gene="yqgB"
                     /locus_tag="N7B39_004552"
     CDS             complement(2029..2160)
                     /gene="yqgB"
                     /locus_tag="N7B39_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HGU2424386.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            complement(2254..2397)
                     /gene="yqgG"
                     /locus_tag="N7B39_004553"
     CDS             complement(2254..2397)
                     /gene="yqgG"
                     /locus_tag="N7B39_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HGU2424387.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(2508..2759)
                     /gene="yqgD"
                     /locus_tag="N7B39_004554"
     CDS             complement(2508..2759)
                     /gene="yqgD"
                     /locus_tag="N7B39_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HGU2424388.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            2815..3969
                     /gene="metK"
                     /locus_tag="N7B39_004555"
     CDS             2815..3969
                     /gene="metK"
                     /locus_tag="N7B39_004555"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HGU2424389.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
BASE COUNT         1050 a         1128 c         1007 g          951 t
ORIGIN      
        1 cgatttacgt aaataaatcg acccgccagc agaataatct taattattca tcttcaagat
       61 aggtataacc gtacaaacct gcctcgaact cttcaaggaa ctgttgttgc agttcagcat
      121 caagatcggt tttcttcact tgatcacgga actgagttaa cagcgttttc ggatcgagct
      181 gtacatattg cagcatgtcc gccacggtat cgccttcgtc agacagttcc acttctacac
      241 taccgtcagg gaagacgaac acgtcaaccg cttcggtatc accgaacagg ttgtgcatgt
      301 tgccgaggat ctcctgatat gcgccgacca taaagaaacc gagcatcggc ggattctctg
      361 gatcgtactc cggcataggc attgtcgtgg caataccatc accatcgata tagtggtcga
      421 tagcaccgtc agagtcacag gtaatatcca gcagcacagc gcggcgttcc ggcacttgat
      481 ccagcccttc cagcggcaga accgggaaca actggtcgat cccccacgca tccggcatcg
      541 actggaacag cgagaagttg acgtacattt tgtccgccat acgttcctgc agctcgtcga
      601 taatcggacg atgagcacgg ttttgcggat ccagctgctt ttgcacttca tggcacatgc
      661 tcaaataaag ctgctcagcc catgcacgtt cttgcaggct aaaggtgccg gaagagtagc
      721 cgatatgaat gtcgtgcaga tccatctgac tgtcgtgtaa ccattcacgc agagaacggc
      781 gagttcccgg ttcgtgcatc tcctgccagg tttcccacat gctttgcagc gcgcgcggcg
      841 catcttctgc aggcgcggtc ggcaccgtgt attcgttacg ttccacgccg atgatattag
      901 acaccagcac ggtgtgatgc gcagtcactg cacgacccga ttcggtgatt accgtcggat
      961 gcggcagacc gttttcttca cacgcatcac caatcgccca gatgatgttg ttggcatatt
     1021 cattgaggcc gtagttcacc gaacagtcgg actgcgaacg agtaccttca taatccacgc
     1081 ccagaccgcc gccgacgtcg aagcactgaa tattgacgcc cagcttgtgc agctcaacat
     1141 agaaacgcgc ggattcacga acgcctgtcg cgatatcgcg aatattcgcc atctgcgaac
     1201 cgaggtggaa gtgcagtagt tgcaggctgt cgagacgccc ggcttcacgt agggtttcaa
     1261 ccagttgcag tacctgagtc gccgccaggc cgaacttcga tttttccccg ccggaggact
     1321 gccatttacc ggagccctgc gaagccagac gtgcacgcac gcccagacga ggaacgacat
     1381 tcagacgttc tgcttcatcc agcacaatgg cgatttctga catcttctca atgaccagat
     1441 agaccttgtg ccccatcttc tcgccaatta atgccaggcg gatatattcg cggtctttat
     1501 aaccattgca gacgatgacg ctacgggtca tgccagcatg tgccagcact gccatcaact
     1561 cggctttgga accggcttcc agacccagcg gttcgcccga atgaatcagg gactcaatca
     1621 cgcgacggtg ctggttaact ttgatcggat aaacaaggaa gtaatcgccg ttatagccgt
     1681 aggattcccg cgcacgtttg aacgcggcgt taatggaacg caaacggtgc tgcaggatct
     1741 gtgggaaaca gaacagtgca ggcagacgct gaccctgtgc ttcacgagtt ttcactaact
     1801 gcgcgagatc gacgcgagct tccgggacgt ccgggtccgg gcacacgctg atgtggccca
     1861 gctcgttaac gtcatagtag ttattgcccc accaggcaat attgtaagta cgcagcatct
     1921 tgctggcttc ctgggagctc attgcaacct cctgcatgga gcgtagtaca ccgtgttcgc
     1981 ccgctgacga aggcaaaccc atagacatgt cgtcagacat agcgaacctc aaattatttt
     2041 attaagtgta aaacagttaa cgactatcgc agcctggaac tgcgataaca acccataagc
     2101 acatggattt tccagcagtg aatgctgacg ctccaactgc gcaaccggtt tctttttcat
     2161 aacattatta agcacataac cgaacgtaag tgtgacagtt cggcgaaacc acgagaaaac
     2221 tcttgttttt acaagagcgc ccttgttcag tcctcagtaa ctgtaaccag ctcttgaatc
     2281 ctgagaagcg ccgagatggg tataacatcg gcaggtatgc aaagcagaga tgcagagtgc
     2341 ggggaacgaa tcttcaccag aacggtgaga caggttaagc agcagacaac ggttcattat
     2401 ttcgtatcac ctccacggcc gcctgttaag acgaacccac aagccaaaac tctgatttca
     2461 acccggctgg aagtggcaac acgaaagaaa cgtcgtgtgc tttttattta agccgcgcgc
     2521 cgcgttttat accccacaat ggcagaaaat tgcaaaagat aaatacgcag aatgccggca
     2581 ttgtcaggaa aaatttccag ccacgttttt aacagaatga gacacgattc aaaaaaaagt
     2641 ggaaatcggg tgaagaattg acctaaaata gccatccaga tgttaatcca tccataccga
     2701 ttaacactca gactgccagt gtttttaacc tgcagagtcg tggtaggatc cgctaccaca
     2761 gaaaatccac acaacagttt gagctaacca aattctcttt aggtgatatt aaatatggca
     2821 aaacaccttt ttacgtccga gtccgtctct gaagggcatc ctgacaaaat tgctgaccaa
     2881 atctctgatg ccgttttaga cgcgatcctc gaacaggatc cgaaagcacg cgttgcttgc
     2941 gaaacctacg taaaaaccgg catggtttta gttggcggcg aaatcaccac cagcgcctgg
     3001 gtagacatcg aagagatcac ccgtaacacc gttcgcgaaa ttggctatgt gcattccgac
     3061 atgggctttg acgctaactc ctgtgcagtt ctgagcgcta tcggcaaaca gtctcctgac
     3121 atcaaccagg gcgttgaccg tgccgatccg ctggaacagg gcgcgggtga ccagggtctg
     3181 atgtttggct acgcaactaa tgaaaccgac gtgctgatgc cagcacctat cacctatgca
     3241 caccgtctgg tacagcgtca ggctgaagtg cgtaaaaacg gcactctgcc gtggctgcgc
     3301 ccggacgcga aaagccaggt gactttccag tatgacgacg gcaaaatcgt tggtatcgat
     3361 gctgtcgtgc tttccactca gcactctgaa gagatcgacc agaaatcgct gcaagaagcg
     3421 gtaatggaag agatcatcaa gccgattctg cccgctgaat ggctgacttc tgccaccaaa
     3481 ttcttcatca acccgaccgg tcgttttgtt atcggtggcc cgatgggtga ctgcggtctg
     3541 actggtcgta aaattatcgt tgatacctac ggcggcatgg cgcgtcacgg tggcggtgca
     3601 ttctctggta aagatccatc aaaagtggac cgttccgcag cctacgcagc acgttatgtc
     3661 gcgaaaaaca tcgttgctgc tggcctggcc gatcgttgtg aaattcaggt ttcctacgca
     3721 atcggcgtgg ctgaaccgac ttccatcatg gtagaaactt tcggtactga gaaagtgcct
     3781 tctgaacaac tgactctgct ggtacgtgag ttcttcgacc tgcgcccata cggtctgatt
     3841 cagatgctgg atctgctgca cccgatctac aaagaaaccg cagcatacgg tcactttggt
     3901 cgtgaacatt tcccgtggga aaaaaccgac aaagcgcagc tgctgcgcga tgctgccggt
     3961 ctgaagtaat ctttcttcac ctgctttcaa aggccagcct cgcgctggcc tttttctttt
     4021 acctcaccgc ggtggatagg cgttcacgcc gcatccggca aaaaaaccgc ctgcacaata
     4081 acatcattct tcctgatcac gtttcaccgc aaattatcat cacaactgaa accgat
//
LOCUS       DBFIFI010000286         4114 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.286, whole genome shotgun sequence.
ACCESSION   DBFIFI010000286 DBFIFI010000000
VERSION     DBFIFI010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.286"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            51..638
                     /gene="betI"
                     /locus_tag="N7B39_004556"
     CDS             51..638
                     /gene="betI"
                     /locus_tag="N7B39_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HGU2424390.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPRHQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            652..2124
                     /gene="betB"
                     /locus_tag="N7B39_004557"
     CDS             652..2124
                     /gene="betB"
                     /locus_tag="N7B39_004557"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HGU2424391.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPSLEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCNAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGLDNGAWVAPTVFTDCSDEMTIVREEIFGPV
                     MSILTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            2138..3826
                     /gene="betA"
                     /locus_tag="N7B39_004558"
     CDS             2138..3826
                     /gene="betA"
                     /locus_tag="N7B39_004558"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HGU2424392.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAM
                     DLDNWAKEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDCKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHDLPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKMADMIRGKEALPRSTAGYFVANGMPVRAKKMSRDLN"
     gene            complement(3828..>4114)
                     /locus_tag="N7B39_004559"
     CDS             complement(3828..>4114)
                     /locus_tag="N7B39_004559"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424393.1"
                     /translation="FLSSLQEPFSYMLTVSGSEQVTIARNTSGVILKLSGISPAVYQC
                     IHSTFISEIERVSLEFAGEVVTMKHESYPYVLYNDSVPIPGTVTLNLIGS"
BASE COUNT          950 a         1128 c         1203 g          833 t
ORIGIN      
        1 tcaataaccg ctttaataga taaacaacgc tgatgaatgg agtggcaaaa atgcccaaat
       61 tggggatgca gtcgatccgg cgcagacaac tgatcgacgc cacactggaa gcaataaatg
      121 aagtgggcat gcacgatgca acgatcgcgc agatcgcccg ccgtgcaggc gtttctacgg
      181 ggatcatcag ccactatttc agggacaaaa atggtctgct ggaagcaacc atgcgcgata
      241 tcaccagtca gctgcgtgac gcggttttga atcgattaca tgcacttccg cagggcagtg
      301 cagagcagcg attacaggcg attgttggcg gaaacttcga tgaaacgcag gtgagcagtg
      361 cggcgatgaa agcctggctg gcgttctggg ccagcagtat gcatcagccg atgctctatc
      421 gtttacagca ggtcagcagc cgccgcttgc tgtcgaatct ggtgagcgag tttcgccgcg
      481 aattgccgcg ccaccaggca caggaagcgg gctacggcct tgccgcgctg attgacggat
      541 tatggctgcg cgcggcactg agcggcaaac cgctggataa acctctcgca cactcgctga
      601 cccgtcactt tattactcag catttaccca ccgattaacc gaggagacgt gatgtcccga
      661 atggcagaac agcagcttta tatacatggt ggttatacct ccgccaccag cggtcgcacc
      721 ttcgagacca ttaacccggc caacggtaac gtgctggcga ccgtgcaggc cgccgggcgc
      781 gaggatgtcg atcgcgccgt gaagagtgcc caacaggggc aaaaaatctg ggcggcgatg
      841 accgctatgg aacgctcgcg tattctgcgt cgggccgtcg atattctgcg cgagcgcaat
      901 gatgaactcg ccaaactgga aaccctcgac accggaaaag cgtattcgga aacctcaacc
      961 gtcgatatcg ttaccggtgc ggacgtgctg gagtactacg ccgggctgat cccgtcgctg
     1021 gaaggcagcc agatcccgtt gcgtgagaca tcatttgtct acactcgccg cgaaccgctg
     1081 ggcgtggtgg cagggattgg cgcatggaac tacccgatcc agattgccct gtggaaatcc
     1141 gccccggcgc tggcggcggg taatgcgatg attttcaaac cgagcgaagt cactccgctt
     1201 accgcgttaa agctggctga aatttacagc gaagcgggcc tgccggacgg tgtatttaac
     1261 gtgttgccgg gcgtgggcgc ggagaccggg caatatctga ccgagcatcc gggcattgcc
     1321 aaagtgtcgt ttaccggcgg cgtcgccagc ggcaaaaaag tgatggctaa ctcggcagcc
     1381 tcttccctga aagaagtgac catggaactg ggcggtaaat caccgctgat cgttttcgac
     1441 gatgcggatc tcgatctcgc cgccgatatc gccatgatgg caaacttctt cagctccggt
     1501 caggtgtgta ccaatggcac ccgcgtcttc gttccggcga aatgcaacgc cgcatttgag
     1561 cagaaaattc tggcgcgcgt tgagcgcatt cgcgcgggcg acgttttcga tccgcaaact
     1621 aacttcggcc cgctggtcag cttcccgcat cgcgataacg tgctgcgcta tatcgccaaa
     1681 ggcaaagagg aaggcgcgcg cgtactgtgc ggcggcgatg tactgaaagg cgatggcctc
     1741 gataacggcg catgggttgc accgaccgtg ttcaccgatt gcagcgacga gatgaccatc
     1801 gtgcgtgaag agatcttcgg gccggtgatg tccattctga cctacgagtc ggaagacgaa
     1861 gtcattcgcc gcgccaatga taccgactac ggcctggcgg cgggtatcgt gacggcggac
     1921 ctgaaccgcg cgcatcgcgt cattcatcag ctggaagcgg gtatttgctg gatcaacacc
     1981 tggggcgaat ccccggcaga gatgcccgtt ggcggctaca aacactccgg tattggccgc
     2041 gagaacggcg tgatgacgct ccagagttac acccaggtga agtccatcca ggttgagatg
     2101 gctaaattcc agtccatatt ctaaccagga ggtttatttg caatttgact acatcattat
     2161 tggtgccggc tcagccggca acgttctcgc tacccgtctg actgaagatc cgaatacctc
     2221 cgtgctgctg cttgaagcgg gcggcccgga ctatcgcttt gacttccgca cccagatgcc
     2281 cgctgccctg gcattcccgc tacagggtaa acgctacaac tgggcttacg agacggaacc
     2341 tgaaccgttt atgaataacc gtcgcatgga gtgcggacgc ggtaaaggcc tgggtggatc
     2401 gtcgctgatc aacggcatgt gctatatccg tggcaacgcg atggatctcg acaactgggc
     2461 aaaagaaccc ggtctggaga actggagtta tctcgattgc ctgccctact accgcaaggc
     2521 cgagacccgc gacgtgggcg agaacgacta ccacggcggc gacggcccgg tgagcgtcac
     2581 cacctccaaa cccggcgtca atccgctgtt tgaagcgatg attgaagcgg gcgtgcaggc
     2641 gggctacccg cgcacggacg atctcaacgg ttatcagcag gaaggtttcg gcccgatgga
     2701 tcgcaccgtc acgccgcagg gccgccgcgc cagcaccgcg cgcggttatc tcgatcaggc
     2761 caaatcgcgc ccaaacctaa ccattcgtac tcacgccatg accgatcaca ttatttttga
     2821 ctgtaaacgc gcggtgggcg tcgagtggct ggaaggcgac agcaccattc cgacccgcgc
     2881 gacggcgaac aaagaagtgc tgttatgtgc aggcgcgatt gcctcaccgc agatcctgca
     2941 acgctctggc gtcggcaacg ctgaactgtt ggccgagttt gatattccgc tggtgcatga
     3001 tttacccggc gtcggtgaaa atcttcagga tcatctggag atgtatctgc aatatgagtg
     3061 caaagaaccg gtttccctct accctgccct gcagtggtgg aatcagccga aaatcggtgc
     3121 ggagtggctg tttggcggca ccggcgttgg tgccagcaac cactttgaag caggcggatt
     3181 tattcgcagc cgagaggaat ttgcgtggcc gaatattcag tatcacttcc tgccggtagc
     3241 gattaactat aacggctcga atgcagtgaa agagcacggc ttccagtgcc acgtcggctc
     3301 gatgcgctcg ccaagccgtg ggcatgtgcg gattaaatcc cgcgacccgc accagcatcc
     3361 ggcaattctg tttaactaca tgtcgcacga acaggactgg caggaattcc gcgacgcaat
     3421 tcgcatcacc cgcgagatca tgcatcaacc cgcgctggat cagtatcgtg gccgcgaaat
     3481 cagccccggc acggaatgtc agacggatga gcagctcgat gagtttgtgc gtaaccacgc
     3541 cgaaaccgcc ttccatccgt gcggtacctg caaaatgggc tacgacgaga tgtccgtggt
     3601 tgacggcgaa ggccgcgtgc atgggctgga aggcctgcgc gtggtggatg cgtcaattat
     3661 gccgcagatt atcaccggga atttgaacgc cacgacgatt atgattggcg agaaaatggc
     3721 ggatatgatt cgcgggaagg aagcgttgcc gaggagcacg gcgggatatt ttgtggcaaa
     3781 tgggatgcca gtaagagcga aaaaaatgag tcgtgatttg aactgactca ggaaccgatc
     3841 agattgagag ttaccgttcc aggtatgggg acagaatcgt tatataaaac ataggggtaa
     3901 ctctcatgct tcatagtgac aacctcgcca gcaaattcta agctgactcg ttcaatctcg
     3961 ctgatgaagg tactatgaat gcactgataa accgcaggtg aaatgccact cagcttaaga
     4021 attacgccgc tagtgttcct ggctatggtg acttgttcac tcccgctcac ggttagcata
     4081 tacgaaaagg gttcttgtaa cgaggacaaa aata
//
LOCUS       DBFIFI010000287         3940 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.288, whole genome shotgun sequence.
ACCESSION   DBFIFI010000287 DBFIFI010000000
VERSION     DBFIFI010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3940
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.288"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(1..461)
                     /locus_tag="N7B39_004560"
                     /pseudo
     CDS             complement(1..461)
                     /locus_tag="N7B39_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
     gene            complement(487..1227)
                     /locus_tag="N7B39_004561"
     CDS             complement(487..1227)
                     /locus_tag="N7B39_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000788752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGU2424394.1"
                     /translation="MKNIAAVGVLERIRRLAPQGAVPPYRTVEEWREWQLAEGRKRSE
                     EINRLNHQVRVEKILNRAGIQPLHRKCSFGNYRVQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHDGYDNGQSGEKFLQE
                     LCGVDLLVLDEIGMQRDTRNEQVTLNQIVDRRTASMRSVGMLTNLNHAAMSTLLGDRV
                     MDRMTMNGGRWVNFNWESWRSNVGRQGM"
     gene            complement(1234..2196)
                     /locus_tag="N7B39_004562"
     CDS             complement(1234..2196)
                     /locus_tag="N7B39_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU2424395.1"
                     /translation="MSSKLHGLVWEGCAFTGMILSRVAVMARLADYSNDEGVSWPAIE
                     TIRRQIGARSESTVKSAIAELAKEGWLTKEERKVGGRNVSNIYRLNVEKLEAAAAAAR
                     ESYKPKRKISPAKNDPLTVDPSNIAPSTVDPSNFDGSTVDNKLPIRGAMIDPDPSVLK
                     PDPSDKRSSCPDASQPDPQTAEQNFLTRHPDAVVFSAKKRQWGSQEDLVCAQWIWGRI
                     VSLYEQAASDDGEITRPKEPNWTAWANDVRTMRMLDGRTHRQICEMFGRLQRDSFWVK
                     NIMSPAKLREKWDELVIRLGRSPAQRCVNHISEPDTEIPPGFRG"
     gene            complement(2219..2644)
                     /locus_tag="N7B39_004563"
     CDS             complement(2219..2644)
                     /locus_tag="N7B39_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HGU2424396.1"
                     /translation="MKIKHEHIRMAMNVWAHPDGEKVPAAKITKAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWLDKDTPDAVEKMQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVASAVVLYDQMNRGGPAGNAVVMH"
     gene            complement(2641..2943)
                     /locus_tag="N7B39_004564"
     CDS             complement(2641..2943)
                     /locus_tag="N7B39_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU2424397.1"
                     /translation="MTLKEFIKSLRVGDAKKFAARLGVSPSYLSQMASGRTAISPTRA
                     LMIESATEGQVSRAELRPHDWELIWPEYASGIRLGQTHVVHAEGDCSACLSDGVDS"
     gene            3041..3412
                     /locus_tag="N7B39_004565"
     CDS             3041..3412
                     /locus_tag="N7B39_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424398.1"
                     /translation="MQKKEIRRLRLKEWFKDKTLPPKEKSYLSQLMSGRASFGEKAAR
                     RIEQTYGMPEGYLDAEYAEQPGGSPPHAGLTSNQLELLQIFSAFPEDEQRQIISELKQ
                     KKESMEDLIARWIAAQKCRRA"
     gene            3433..3624
                     /locus_tag="N7B39_004566"
     CDS             3433..3624
                     /locus_tag="N7B39_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stability determinant"
                     /protein_id="HGU2424399.1"
                     /translation="MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDE
                     VERRMAERFAKMRKERSKQ"
     gene            3626..3904
                     /locus_tag="N7B39_004567"
     CDS             3626..3904
                     /locus_tag="N7B39_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGU2424400.1"
                     /translation="MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLS
                     EHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVEVVNVIHARRQFP"
BASE COUNT          965 a         1105 c          877 g          993 t
ORIGIN      
        1 cgttaccggc gcggctttcg gatctgctgg taaattaccc cccgacatgc agtaacgaaa
       61 tttaccgttc tgattaacgc gtgccagccg ccccgttgcg gttaccaccg ccagcgtgga
      121 agcaaccttg cgagtactga cgccgaactt acccgccagt tcctcacacg ttttagcacc
      181 atcctgaccg ataaactcaa ccatcatgtc tgcggtaact tttggagcga cctcttcggt
      241 taccacatcc ggcgcttcag gttgtagtgc ctgcccttcg gttaccccgg cttcaccttc
      301 gacagccaga aaccaggtgt gacccgtttt atcaacaacg ccattttttt gagttcccac
      361 agttcgttaa gaacttcttc acggctgata tcaagccgcg ccgccagttc aacagaattg
      421 gcttttccca tcgctttcag tgcatgcaat acagtttcca tcgaaaattt acctcgtcaa
      481 aaattctcac ataccctgac gtccaacgtt tgaccgccag ctctcccagt taaaattcac
      541 ccaacgacca ccattcatgg tcatacggtc catcacccga tctccgagga gtgtgctcat
      601 cgctgcgtga ttcaggttcg tcagcattcc gacactacgc atcgaagccg ttctgcggtc
      661 gactatctgg ttcagcgtga cctgctcgtt gcgcgtatcc cgctgcatgc caatttcatc
      721 aaggaccaga aggtcaactc cacaaagctc ctgtaaaaat ttttccccgg actggccgtt
      781 gtcgtagccg tcatgcaaca cgctcatgac atcggacacg gtgacgataa tcacgcttct
      841 ccccttcgcc atcagccgat tgccaatcgc tgctgccagg tgatttttac cggtaccagg
      901 tttaccgctg aacacgaagt ttgtacatcc ggtcatcaat tcatcggcaa tggatttcgc
      961 ctggctcaga gcatggcgct gaccgtcgtt ctgcacccgg tagttcccga atgagcactt
     1021 cctgtgaagc ggctggatgc ccgcacggtt caggattttt tcaacccgca cctgatgatt
     1081 caggcggtta atctcctcgc tgcgttttcg tccttcagca agttgccatt cccgccactc
     1141 ctccaccgtc cggtacggtg gaaccgcccc ctgtggtgca agtctgcgaa tacgttcaag
     1201 aaccccaact gccgcaatgt ttttcatgac acgtcacccc ctgaatcccg gcggtatttc
     1261 agtgtccggt tcagaaatgt gattcacgca acgctgcgcg ggcgaacgcc ccaggcggat
     1321 aaccagttca tcccattttt cccggagttt tgccggactc atgatgtttt ttacccagaa
     1381 cgaatcccgc tggagacgcc caaacatttc acaaatttgt ctgtgagttc tgccatccag
     1441 catccgcatt gtccgcacgt cattggccca tgcagtccag ttgggttctt tcggtctcgt
     1501 gatctcgcca tcatcgctgg ccgcctgctc gtaaagactc acgattcgtc cccagatcca
     1561 ctgtgcgcac accaaatctt cctgacttcc ccactggcgt tttttcgcac tgaacacaac
     1621 cgcgtcaggg tgtcgggtta aaaaattctg ttcagccgtc tgcgggtccg gttgcgaagc
     1681 gtccggacaa gaagatcttt tatctgacgg atcaggtttt aatactgacg gatcggggtc
     1741 aatcatcgcc cccctaatcg gcagtttgtt atcaacagtt gatccatcaa aatttgacgg
     1801 gtcaaccgtt gagggggcaa tatttgacgg gtcaactgtt aacgggtcat tttttgccgg
     1861 gctaattttt cttttcggtt tatatgactc acgcgccgcc gcagcagctg cttcgagttt
     1921 ttccacatta agccgataga tattgcttac attacgccca ccgaccttac gctcttcctt
     1981 cgtcagccag ccctctttcg ccagttctgc aatagccgat ttcactgtgg attcacttct
     2041 tgcaccgatc tgacgccgga tagtttcaat ggcaggccat gacacgccct cgtcattgct
     2101 gtagtctgca agacgggcca taaccgccac cctggataag atcatgccgg tgaaggcgca
     2161 cccttcccag acaagaccat gaagcttgct gctcataaaa cccccgaaca ccgtgctttt
     2221 agtgcatcac cacagcattc cctgccgggc cgccgcgatt catctggtca tacaaaacaa
     2281 ccgctgacgc aacaaaatca tcgacatcct tcaccagccg atccctccgt tcgacgatct
     2341 cacggtaata ttcagaactg tggctgcgca tacgggccac cagcaaaggc ggcatcgcct
     2401 tttcgatcgc cggtaacaga gcctgcattt tttcaacagc atcaggggtg tctttatcca
     2461 gccaacggaa aattttctgg gtattacggg ccagggcttc cggatggctg tcgtcataca
     2521 gttccgggaa cgtcattccc agctcgaaat acgctttggt aattttcgca gccggtactt
     2581 tttcgccgtc cggatgcgcc cagacattca tcgccatgcg gatgtgttca tgcttgattt
     2641 tcatgaatca actccatcag ataagcatgc actacaatca ccttcagcat gaactacatg
     2701 tgtttgcccc aaacgaatgc cgctcgcata ctcaggccaa ataagctccc aatcatgggg
     2761 tcgtagctcc gccctactta cttggccttc cgtcgcagat tcgatcataa gggcgcgggt
     2821 tggagatata gctgttcgtc cagacgccat ttgcgataag taagatggcg atacaccaag
     2881 tctggccgcg aatttcttag catcaccaac ccttaatgat ttaataaact cttttaatgt
     2941 cataccttcc tcggtttagt gtttttttgc gagtttagtg tttaataaac cattaagtca
     3001 agtatttgct tgtttagtga ttactaaaga taattaccac atgcaaaaaa aagaaattcg
     3061 ccgtttacgt ctcaaggagt ggtttaaaga taaaactctg ccacccaaag agaagagcta
     3121 cctatctcaa ctaatgagtg ggagagcctc gtttggagaa aaggctgcca gaagaataga
     3181 gcaaacatac gggatgccgg aagggtatct ggatgcggaa tacgcagaac aaccgggggg
     3241 ttctccacca catgcagggt taacgtctaa tcaactggaa ttattgcaga ttttttcagc
     3301 cttccctgag gatgagcaac gccagataat cagcgagtta aagcagaaaa aagaatcaat
     3361 ggaagatctc atagcgagat ggattgcggc gcaaaaatgc cgccgcgcct gagttataaa
     3421 accggaggaa acatgaatag agccctttca ccaatggttt ctgaatttga aaccattgaa
     3481 caagaaaaca gttacaacga atggctgcgt gcgaaagtag caacgagcct tgcagatccg
     3541 cgcccagcaa ttccccatga cgaagttgag cgcagaatgg cagaacgctt tgctaagatg
     3601 cgcaaggaac ggagcaagca gtaaaatgtt acccgtgtta tggcttgaaa gcgcagatac
     3661 cgacctagat gatataacta gttatattgc tcgtttcgac atagatgcgg ctgaacgctt
     3721 atggcagcga ttaaggggtt gtgtgctgcc gttatccgaa catccgtatt tatacccacc
     3781 aagcgacaga gtacctggct tgcgtgagat tgtagcccac cctaactata taattctata
     3841 ccgcgtaaca acatcaagcg ttgaagtagt aaacgtgatc cacgcaagac gccagtttcc
     3901 ctaactttca ctaccaatag aaacataaca accgcaacga
//
LOCUS       DBFIFI010000288         3927 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.289, whole genome shotgun sequence.
ACCESSION   DBFIFI010000288 DBFIFI010000000
VERSION     DBFIFI010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3927
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.289"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(58..2391)
                     /locus_tag="N7B39_004568"
     CDS             complement(58..2391)
                     /locus_tag="N7B39_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primase-helicase zinc-binding domain-containing
                     protein"
                     /protein_id="HGU2424401.1"
                     /translation="MSGMKVSQAEKAARGHWSRILPALGVNVLKNRHQPCPVCAGKDR
                     FRFDDQEGRGTWFCNQCGAGDGLALVSKVLDVGISEAADRINGIIGNLLPVSQGMLES
                     GSPEKEDGRKAAAVLAARLFDKSRQTTGNAYLTSKGFPALPCRELTAMHKVGGVAFRA
                     GDLVVPLYADGELVNLQLINANGGKCFLKGGQVKNAFYLVEGTAKAAKRLWIAEGYAT
                     ALTINYLTGDAVMVAFSSVNFLSLASIACSEYPTHQIIIAADRDLNGAGQTRGAAVTG
                     ACNCTMALPPVFGDWNDAFTQNGEEATRHAIHEVIKPAVASPFDTMSEAEFTALSVSE
                     KAQRVVDHYKNSLAVDPNGQLLSRYEAGAWKVIYYADFARDVAALFQRLDAPFSSAKI
                     ASLVETLKLIVPQQQNPARQLIGFRNGVLDTRTGLFSPHDKKHWLRTLCEVDYTQPVD
                     GESLETHAPAFWRWLDRAAGFNPEKRDIILAALFMVLANRYDWQLFLEVTGPGGSGKS
                     ILAEIATMLAGEDNATSATIEMLESPRERAALIGFSLIRLPDQEKWSGDGAGLKAITG
                     GDAVSVDPKYQNAYSTHIPAVILAVNNNPMRFTDRSGGVSRRRVILHFPDQIAPEERD
                     TQLKEKIASELAVIVRQLMQRFSDPMSARTLLQSQQNSDEALTIKRDADSAFDFCGYL
                     EVLPDTTGMFMGNANIVPRQPRTYLYHAYLVYMEANGYKNTLSLTMFGKGLPLMLKEY
                     GLQYEKRRTNQGMQTNLALREESNADWLPKCDEFAAK"
     gene            complement(2405..2728)
                     /locus_tag="N7B39_004569"
     CDS             complement(2405..2728)
                     /locus_tag="N7B39_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5375 family protein"
                     /protein_id="HGU2424402.1"
                     /translation="MICCESLLALRAALYRRAVACAWLALSNHQERYSGLTLAELEDA
                     IARELEGFYLRQHGQQRGLEIACALLSDLMESGPLKACPVLSLLGMTVMDELCSRHLN
                     KPALH"
     gene            complement(2728..2949)
                     /locus_tag="N7B39_004570"
     CDS             complement(2728..2949)
                     /locus_tag="N7B39_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424403.1"
                     /translation="MNQFEISYDDVVRLKHLRNVGEYVTGMAALQDCYEKPAGAQCEQ
                     LVSLIYLMTEQLDGVVQRCHDDLMNAEVA"
     gene            complement(2946..3503)
                     /locus_tag="N7B39_004571"
     CDS             complement(2946..3503)
                     /locus_tag="N7B39_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HGU2424404.1"
                     /translation="MMIPAYIKYLYSGLLTVVISRYSFSAVAKSAAGIGVPYNLLATI
                     DAPCVFFYVVAQAQPFSGLWCLCLHHGSIEIMVVRAGQPSGWPVSNKAGYANPVRAAT
                     SEIGVSGGSNNRYLLEAAIMATILTPSHPQYVFVFAAIRRADTHPRICMLRTVSCDER
                     SARRLLVRDYVLSLSARLPAGEVTL"
     gene            complement(3500..3760)
                     /locus_tag="N7B39_004572"
     CDS             complement(3500..3760)
                     /locus_tag="N7B39_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU2424405.1"
                     /translation="MRNISVSAPAPAAPLFPVSNHHERFLRLPEVMHLCGLSRSTVYD
                     LISRDAFPKQIPLGGKNVAWAQSEVSAWMADRISARGRGCDA"
BASE COUNT          961 a         1060 c          980 g          926 t
ORIGIN      
        1 attttgagat aatttcctgt agcggattta agaccggcac agccggtctt aggtcagtta
       61 tttcgctgca aactcatcgc attttggcaa ccagtcagca ttgctttcct ctcttagggc
      121 cagattagtc tgcattcctt gattggtccg tcgtttctca tactgcagcc catattcctt
      181 gagcattaac ggtagcccct tgccaaacat ggtcagactg agcgtatttt tatagccgtt
      241 agcctccatg tagaccagat aggcatggta gaggtaagtt cgaggctgac gtggaacaat
      301 attagcgttc cccataaaca tgcccgtggt gtcaggtagg acctcaaggt agccgcaaaa
      361 atcaaaagct gaatcagcat cacgtttgat ggtgagcgct tcatcggagt tctgctgtga
      421 ctgaagcaat gtcctggcac tcattgggtc gctgaaacgc tgcataagct ggcgaacaat
      481 caccgctagc tcactggcaa ttttctcttt gagctgggta tcgcgttcct ccggggctat
      541 ctggtcgggg aaatgcagga tcacccttcg gcgtgaaact ccaccactac gatcagtgaa
      601 gcgcatcgga ttattgttca cagccaggat gaccgccggg atgtgggttg aataggcgtt
      661 ctgatatttg ggatcaacgg ataccgcatc gccgccagtg atggctttta gtccggcccc
      721 gtcaccgctc cacttttcct ggtcgggaag tcgaatcagt gaaaaaccta ttaacgcagc
      781 tcgttctctt ggcgactcaa gcatttcaat ggttgccgag gtcgcgttat cttcacccgc
      841 cagcatggtt gctatttcag caagaatact ctttccactt ccgccagggc cagtgacctc
      901 cagaaacagc tgccagtcat aacggttagc cagcaccata aacaatgcag ccagaataat
      961 gtcccgtttt tcaggattaa aacctgcggc acgatccagc cagcgccaga atgccggggc
     1021 atgggtttcc agtgactcac cgtcaacggg ctgcgtgtaa tccacctcgc acagcgtacg
     1081 taaccagtgc ttcttatcgt gcgggctgaa caatcctgtc cgggtatcga gcacaccgtt
     1141 gcgaaatccg ataagttgcc gcgccggatt ctgctgttgc ggaacgatca gtttgagggt
     1201 ttccacgaga gacgcaattt tcgcggatga aaaaggtgcg tcgaggcgct gaaacagcgc
     1261 agcgacatca cgggcaaaat cggcgtaata gataactttc caggcccccg cctcatagcg
     1321 tgaaaggagc tgcccgttcg ggtctactgc cagtgaattt ttatagtgat ccactaccct
     1381 ctgcgctttt tcgctgacgc tcagcgcggt aaattcagct tcgctcattg tgtcgaaggg
     1441 gctggcaaca gctggtttta ttacttcatg aattgcatgc cgggtggctt cttcgccgtt
     1501 ttgcgtgaat gcatcgttcc agtcaccaaa cacaggcggg agcgccattg tgcaattgca
     1561 ggccccggta acagctgcgc cccttgtttg ccccgcaccg ttgagatcgc ggtcagcagc
     1621 aataattatc tggtgcgttg ggtactcact gcaggcaatg ctcgctaggg aaaggaaatt
     1681 gacggacgaa aaggccacca tgacagcatc gccagtcaga tagttgatag taagtgcggt
     1741 ggcatatcct tccgctatcc agagccgttt ggctgctttg gcagtacctt caaccaggta
     1801 aaaggcattc ttaacctgac cgcctttaag gaagcatttg cccccattag cgttgattaa
     1861 ctgcagattt accagctctc catctgcata caatggaacg acaagatctc ccgcgcgaaa
     1921 tgccacacca ccgactttat gcatagcggt taattcccgg caaggcagtg caggaaaccc
     1981 tttactcgtc agataggcat tgccagtggt ctggcgggac ttatcaaaca aacgggcagc
     2041 cagcactgct gcagcttttc tcccgtcctc tttttcagga gaaccagatt caagcattcc
     2101 ctgagatact ggcagcaggt ttccgataat gccgtttatt ctgtctgccg cttcactaat
     2161 gcctacatcc agtactttac ttacaagcgc caggccatca cctgccccgc actggttaca
     2221 gaaccacgtt ccccgccctt cctggtcatc aaatcgaaag cggtctttcc cggcacagac
     2281 cgggcagggc tggtgccgat ttttcagtac atttacgccc agcgcaggta aaattcttga
     2341 ccagtgaccc cgagctgctt tctcagcctg gctaactttc attcctgaca ttgtgcggcc
     2401 ctccttagtg cagcgctggt ttgttgaggt gacgggaaca aagttcatcc atgaccgtca
     2461 ttccgagcag tgagagaacc ggacaggctt taagcggccc cgactccatc agatccgaaa
     2521 gcaacgcgca ggcaatttcc agccctcttt gctgcccatg ctggcgcaga tagaaccctt
     2581 ccagctcgcg ggctatggca tcttcaagtt cagcgagcgt caggccggaa taacgttcct
     2641 gatgattgct tagagccagc caggcacagg caacggcacg gcgatacaga gcggcacgaa
     2701 gtgccagaag agattcacag catatcatca ggccacctcc gcattcatca ggtcgtcatg
     2761 gcagcgttgc accactccat ccagttgctc tgtcatcaga taaatgaggg aaaccagttg
     2821 ttcgcattgg gcacctgctg gcttttcata acagtcctgc agggctgcca tgccagttac
     2881 atactcaccc acattgcgta aatgtttcag ccttacaaca tcgtcgtagg aaatctcaaa
     2941 ctggttcata gcgtcacctc cccggcaggc agccgtgcag aaagggagag tacataatct
     3001 cggactagca aacggcgcgc ggaacgctca tcgcaggaga cagtgcgaag catacagatg
     3061 cgggggtgag tgtcagcacg gcggattgcg gcaaacacaa agacatattg cgggtgtgac
     3121 ggggtgagga ttgtagccat gatggcagcc tccaataagt agcggttatt gctaccaccg
     3181 gaaacgccaa tttcactggt ggcagcccga acggggttgg cgtaaccggc cttattggaa
     3241 accggccagc ccgaaggctg ccccgcccgg actaccatta tctcgataga accatggtgt
     3301 aagcataaac accacagccc tgaaaatggt tgtgcctgag ctacgacgta aaaaaagacg
     3361 caaggggcgt caattgtcgc caataagtta tacggaacgc caattccggc tgccgatttt
     3421 gcgacagcgg aaaaactata cctggaaatg acgacagtaa gcaagccaga ataaaggtat
     3481 ttaatgtatg caggtatcat catgcatcac atccccgccc acgggcgctg atgcggtccg
     3541 ccatccatgc gctgacctca gactgtgccc aggcgacatt tttaccgcca agcgggatct
     3601 gcttcggaaa cgcatcgcgg ctgatcaggt cgtaaacggt cgaacgggac aacccgcata
     3661 agtgcatcac ctcaggcagg cgtaaaaatc gctcatgatg gttcgataca ggaaataatg
     3721 gcgcagcagg agctggcgca gacacagaaa tattacgcat ttatctaccc tctttaattt
     3781 ccacaacagt ccggatgaat ttatccgggt tcaggtagtt ccttattatg tgtatataaa
     3841 ccacctgcgc acgcattttt tatttagcgt cacatattga ttttatggaa tttaaagccc
     3901 gattaaacca tataaaatga cataata
//
LOCUS       DBFIFI010000289         3879 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.290, whole genome shotgun sequence.
ACCESSION   DBFIFI010000289 DBFIFI010000000
VERSION     DBFIFI010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3879
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.290"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..827)
                     /locus_tag="N7B39_004573"
     CDS             complement(<1..827)
                     /locus_tag="N7B39_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HGU2424406.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVREKL
                     DTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLG"
     gene            complement(883..1215)
                     /locus_tag="N7B39_004574"
     CDS             complement(883..1215)
                     /locus_tag="N7B39_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HGU2424407.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTSS
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            complement(1225..2544)
                     /locus_tag="N7B39_004575"
     CDS             complement(1225..2544)
                     /locus_tag="N7B39_004575"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HGU2424408.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPGDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVPAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            complement(2525..>3879)
                     /locus_tag="N7B39_004576"
     CDS             complement(2525..>3879)
                     /locus_tag="N7B39_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU2424409.1"
                     /translation="SFFRLSHRPSWRYLGIGEEEARAFSREVEAAWKEFAEDDCCCID
                     VERKRTFTMMIREGVAMHAFNGELFVQATWDTRPSRLFRTQFRMVSPKRISNPNNTSD
                     SRNCRAGVQINDSGAALGYYVSEDGYPGWMPQKWTWIPRELPGGRASFIHVFEPVEDG
                     QTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKAMYAATIESELDTQSAMDFILGANSQ
                     EQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLMPGDSLNLQTAQDTDNGYSVFEQSLL
                     RYIAAGLGVSYEQLSRNYAQMSYSTARASANESWAYFMGRRKFVASRQASQMFLCWLE
                     EAIVRRVVTLPSKARFSFQEARSAWGNCDWIGSGRMAIDGLKEVQEAVMLIEAGLSTY
                     EKECAKRGDDYQEIFAQQVRETMERRAAGLKPPAWAAAAFESGLRQSTEEEKSDSRAA
                     "
BASE COUNT          768 a         1208 c         1061 g          842 t
ORIGIN      
        1 ttccccagca ccatcgtgtt gtccggcagg aagttctttt tgacgccgtt ttccacgtac
       61 tgtccggaat acacgacgat ggccacatcg ccatacattc ccttataaga caccgctttg
      121 cccaggtctt ttaccgctgt ctccagttcg gaatgagagc cgcgacgggt atccagcttc
      181 tccctgacgg ctttgaagga acggaacagc gcccagcctt tcgggtcaaa cacgatgata
      241 ttcaccacgc cgctggcgtt cagcgcgtag gcttcgatat cgtcggtcgg gtcatacgtg
      301 gacttgtcac gcttgctcca ctccgtgccg ccggactgcg tgatgttgtt cgccgcactg
      361 cggcccatat ccacctcaac cggatcgaag gcttcaccgg tcatggtgta tttgcccttg
      421 agcacggcag aaactgcctg catctcttcg acctgagcaa tggccagctc ttcgtcacgc
      481 atgttctgca tgatgatgcg acggcggcgg taagccgggt ccgccagatt ctgcggatct
      541 tcatccggca ggcgacgcag ggtcatctgc ggattcactt catgcttcgg cttgacatat
      601 cccggcgtaa attcagaggt ggagccgcca cgggaacgga taacctcacc agaaacaatc
      661 ggcgaaacgt acagcgccat gtttaccagt cccggaattt gtgagagata gactttctcc
      721 gtggtgaagg gatagctctc acggaaaaag agacgcagaa acagcggatc aaacttaaat
      781 ttctgctcat ttgccgccag cagttgggcg gttgtgtaca tcgacataaa aaaatcccgt
      841 aaaaaaagcc gcacaggcgg cctttagtga tgaagggtca ggttaaacga tgctgattgc
      901 cgttccggca aacgcggtcc gttttttcgt ctcgtcgctg gcagcctccg gccagagcac
      961 atcctcataa cggaacgtgc cggacttgta gaacgtcagc gtggtgctgg tctggtcagc
     1021 agcaaccgca agaatgccaa cggcagcacc gtcggtggtg ccatcccacg caaccagctt
     1081 acggctggag gtgtccagca tcagcggggt cattgcaggc gctttcgcac tcaatccgcc
     1141 gggcgcggtt gcggtatgag ccgggtcact gttgcccagc ggctggtaat gggtaaaggt
     1201 ttctttgctc gtcataaaca tcccttacac tggtgtgttc agcaaatcgt taacggcatc
     1261 agatgccggg ttacctgcag ccagcggtgc cggtgccccc tgcatcagac gatccagcgc
     1321 agtatcactg cgcgcctgtg cactctgtgg tgctgcggcc agaatgcggc gggccgtttc
     1381 cacggtcata ccgggggttt ctgccagcac gcgggcctgt tcttcgcgtc cgtgagcctc
     1441 ctcacagttg aggatcccca taatgcggct gttttctgcc gcaaccgcag cggtgatctg
     1501 cgcgttcacg tccggctgcg ccgcgctggc gttttcgccc tccgtcgctg gcaccacgcc
     1561 agtaacgtca gcctgcgaag cagtggctga aacagttgtt gattgagtct ctttggtcat
     1621 tcgccctcct gagagacggg atttacgtgc atccagtgca tcacgcataa cggtgatcgc
     1681 atcggtgctg ttgacaagtt catcagccag tccggcatca atggcctcct gaccgctgta
     1741 cactgcagcc tcggtatcca gcacagcctg cacggacagg ccggtatatg ccgacacctt
     1801 ctgcgcaaac atccggcggg ttgcatccat ccgggactgc agtgtttccc ggacatcacc
     1861 cggtagatgg ctgtaggggt tgccatccac cttatggctg ccgctgtaaa tcagcgtgat
     1921 ttccacgccc tgtttctcca gggcagcacc gtaattactg tgagccatca tgacgccgat
     1981 ggagcctgtc cgggcggtct gcgtgaccag acgccgggag gcggcgctgg caagcagctg
     2041 acctgcactg cagttcatgt cgttggccag cgcccatacc ggttttatgt ctcgcacacg
     2101 ggcgatgatg tcagcacagt caaatgctcc cgccaccatc ccgcccggtg tgtccatatc
     2161 gagcagaatg ccgtccacca tcggatcgct ggcagcctgt tgcagacggg cgataatgcc
     2221 gttgtaaccg gtcattcccg aatacggctg cagcgcccgc gtccggctga ccagcgtacc
     2281 ggacaccggc agcacggcga tgccgttcat gacctgataa ctccgggcct gtcgtggtcc
     2341 gtcatcatca ccggataacg ccagcgccgc gggtgcctct ccggcagtca ggctgtcgcc
     2401 ggatactgca tccgtcaggc ggctgatccc aagctggcct gcaagcgcac aaaagaaaac
     2461 ccgcgcatag gcgggttcaa gcatcagcgg ctcattaaaa gccatgctgg caatatgcgg
     2521 gagattacgc agctctgctg tcactcttct cctcctctgt tgattgtcgc agcccggatt
     2581 caaatgccgc agccgcccag gcgggcggtt taagaccagc tgcgcggcgc tccatcgttt
     2641 cacggacctg ctgggcaaaa atttcctgat agtcgtcacc gcgtttcgcg cactctttct
     2701 cgtaggtgct cagtccggct tctatcagca tcaccgcttc ctgaacttct ttcagaccat
     2761 cgatggccat acgaccggag cctatccagt cgcagttccc ccaggcactg cgggcttcct
     2821 gaaagctgaa gcgcgctttt gaaggtaacg tcaccacgcg gcgaacgatg gcctcttcca
     2881 gccagcacag aaacatctgg ctcgcctgac gggatgcgac gaattttcgc cgccccataa
     2941 agtacgccca cgactcgttc gcactggccc gtgccgtgga gtagctcatc tgggcgtaat
     3001 tccgggaaag ctgctcatac gagacaccca gcccggcagc gatataccgc agcagtgact
     3061 gctcaaacac ggagtagccg ttatccgtgt cctgagccgt ctgcaggttc agtgagtccc
     3121 ccggcatcag gtgcggcact tttgcgcctc ccagacggac cggtgctgcg gcgtaatacg
     3181 cggcaatttc accaatccag ccggtcagcc tttcccgctg ctcctgactg ttcgcgccca
     3241 gaataaaatc catcgctgac tgcgtatcca gctcactctc aatggtggcg gcatacatcg
     3301 ccttcacaat ggcgctctgc agctgcgtgt tctgcagcgt gtcgagcatc ttcatctgct
     3361 ccatcacgct gtaaaacaca tttgcaccgc gggtctgccc gtcctccacg ggttcaaaga
     3421 cgtgaatgaa cgaagcacga ccgccgggta actcgcgggg tatccatgtc catttctgcg
     3481 gcatccagcc aggatacccg tcctcgctga cgtaatatcc cagcgccgca ccgctgtcat
     3541 taatctgcac accggcacgg cagttccggc tgtcgctggt attgttcggg ttgctgatgc
     3601 gcttcgggct gaccatccgg aactgtgtcc ggaacagtcg cgagggacgg gtatcccagg
     3661 tggcctgaac gaacagttca ccgttaaagg cgtgcatggc cacaccttcc cgaatcatca
     3721 tggtaaacgt gcgttttcgc tcaacgtcaa tgcagcaaca gtcatcttcg gcaaactctt
     3781 tccatgccgc ttcaacctcg cgggaaaagg cacgggcttc ttcctccccg atgcccagat
     3841 agcgccagct tgggcgatga ctgagccgga aaaaagacc
//
LOCUS       DBFIFI010000290         3798 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.291, whole genome shotgun sequence.
ACCESSION   DBFIFI010000290 DBFIFI010000000
VERSION     DBFIFI010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.291"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(47..151)
                     /locus_tag="N7B39_004577"
     CDS             complement(47..151)
                     /locus_tag="N7B39_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424410.1"
                     /translation="MSDDISLAMEGALAVIAVVGVYCLVVFLMDRLGN"
     gene            complement(267..854)
                     /locus_tag="N7B39_004578"
     CDS             complement(267..854)
                     /locus_tag="N7B39_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HGU2424411.1"
                     /translation="MTTITKDRLLTIQHWRETYGPGSNVVLPAEEAEELARIALASLA
                     AVSDERAAYELFMEKRFGESVDRRRAKNGDRDYMVWDMALGWIIWCHRAAMLQAGNFR
                     ENKGSSTNNFREISETSTNYPVTPDGWISCSERMPDDGQHVIILCDGAFVLYAQYRDG
                     EFFDVVRNGDEFFETQSRNVTDWMPLPEPPQEVRQ"
     gene            complement(857..1048)
                     /locus_tag="N7B39_004579"
     CDS             complement(857..1048)
                     /locus_tag="N7B39_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424412.1"
                     /translation="MNGQISIVRPGACDDREIRMIIRLAMGKTITALITPENLALALT
                     GKSDMPVELKLRNVEIKVK"
     gene            complement(1050..1487)
                     /locus_tag="N7B39_004580"
     CDS             complement(1050..1487)
                     /locus_tag="N7B39_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HGU2424413.1"
                     /translation="MGKIDYQALREAAQNYQSTLAWYQATPDSPNAERDCDAALAAFK
                     RHIRHREADIIADLLDGLEEAKSQLNEQREYYEGVISDGSKRIAELEAREVQLPTRYD
                     LRYGHPINADERHVMIPKENGSWLYLIDLEHALRVAGIRIKGE"
     gene            complement(1474..1791)
                     /locus_tag="N7B39_004581"
     CDS             complement(1474..1791)
                     /locus_tag="N7B39_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424414.1"
                     /translation="MTITKQRVEEIISRIEMYGHGAGYIADEVNDLAILALNLSNIAN
                     LKRYELDMGGCDSCGQDCGADMTEDPDGDYVLFDDVVKLFEFDTTTQKLEIPAKEAAS
                     GQD"
     gene            complement(1745..2062)
                     /locus_tag="N7B39_004582"
     CDS             complement(1745..2062)
                     /locus_tag="N7B39_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4752 family protein"
                     /protein_id="HGU2424415.1"
                     /translation="MNIDPAITIDMALNAGLALLGYFYIMFCSGRWLSLLFMKKWNKR
                     RKQEQRQKAMDAFFEAFGIDGMEPGDPARAISRGGVVILVYRSEEKNDDHKTTCRRNH
                     IPH"
     gene            complement(2052..2354)
                     /locus_tag="N7B39_004583"
     CDS             complement(2052..2354)
                     /locus_tag="N7B39_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HGU2424416.1"
                     /translation="MRVRVYIAGPMTGYENFNREAFHKTEEVLKREGHTVLNPAVLPD
                     GLTQPHYMDICMAMLRCVDAVYMLKGWQQSAGAGAELALEEKPGHAVIFQEVGSEY"
     gene            complement(2351..2668)
                     /locus_tag="N7B39_004584"
     CDS             complement(2351..2668)
                     /locus_tag="N7B39_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGU2424417.1"
                     /translation="MSGWKKWRWAEILILRQCAGTMRVESIGYLIGRSESAVRTKARE
                     LGISMMLRGDYHQSAKCSQRDIELAWQLHQRGVPRREIAEKFGMKLGAVNNYVYFDRR
                     VQE"
     gene            complement(2665..3381)
                     /locus_tag="N7B39_004585"
     CDS             complement(2665..3381)
                     /locus_tag="N7B39_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HGU2424418.1"
                     /translation="METVSDALKALKRASSHVVAARLGISREEAVNELWKLKRRGEAD
                     NKGAIWWLTQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMAISKGCLIRVNQDGKFRYCIPGVDLPAEPKAASVAETEGKALPQPAGVALPVQETA
                     AQEEIKTEAVEDIVKLQQSFTEAKADDLILPSLHVANRELRRAKSNVQKWERVCAALR
                     ELNKHRDILRDITATREQQR"
     gene            complement(3415..>3798)
                     /locus_tag="N7B39_004586"
     CDS             complement(3415..>3798)
                     /locus_tag="N7B39_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HGU2424419.1"
                     /translation="LAEFVALISESGANPFGLTVDAVMEEYRRWRNESWRYDGSDKYP
                     WPQPVLYHICLEMRDRGIERQMTEGELKRLAERQLTKWAKQVGNGMSIPPIRRQLASP
                     KCPQGPTPIELLKQEYERRKAAGFV"
BASE COUNT          865 a         1127 c          774 g         1032 t
ORIGIN      
        1 gcaaaccgtt tttacccgat atgggaattc ccatatcgta atgaattcag ttccctagtc
       61 gatccatcaa aaacacaacc aggcagtaaa cgcccacaac agcaataaca gccagcgcac
      121 cttccattgc cagtgaaata tcatccgaca tattccctcc tttggtgtga atcccggcga
      181 acgtttttac ccccaccgac aaataacata tactaaaaaa tcaatagcta tagcaacgcc
      241 tgtaattgca aaggcttcag gccagatcat tggcgcacct cctgcggcgg ttctggtagc
      301 ggcatccagt cggttacatt gcggctctgt gtttcgaaaa attcatcacc attacggact
      361 acatcaaaaa actcaccgtc tcgatattgc gcataaagaa cgaatgcgcc atcacataaa
      421 ataattacgt gctgaccatc atccggcatt cgctcactac agcttatcca accatccgga
      481 gttaccggat agttggttga cgtttccgag atttcccgaa aattattggt tgacgaaccc
      541 ttattttccc gaaagtttcc agcctgaagc atggcggcgc ggtgacacca gataatccag
      601 ccaagcgcca tatcccatac catgtaatct ctatcgccat tttttgctct gcggcgatct
      661 acagattctc cgaaacgctt ctccataaat aattcatagg ctgctcgttc atccgatact
      721 gctgccagtg atgccagcgc aatccgtgcc agctcttccg cttcttctgc tggaagcaca
      781 acgttgctac ccggtccgta tgtttcgcgc caatgctgga ttgtcagcag tcgatctttg
      841 gtaatagtgg tcatagctat ttcaccttaa tctcaacatt tcgcagcttt agctctactg
      901 gcatgtctga ctttcctgtt aatgctaatg cgagattttc tggagtaatg agagcagtta
      961 ttgttttccc catcgccaga cgaataatca ttcgtatttc gcgatcgtca catgctcctg
     1021 gtcgaacaat tgatatttgt ccgttcatct cactctcctt tgatgcgaat gccagcgacg
     1081 cgtaatgcgt gttctaggtc aatcaggtaa agccaactgc cattttcttt aggtatcatg
     1141 acatgtcgct catctgcatt tatcgggtgt ccatatcgaa ggtcgtagcg agtcggtaat
     1201 tgaacttccc gcgcttccag ttcagcaata cgcttgctcc catcagagat aacgccttcg
     1261 taatactcac gctgctcgtt gagttgtgat tttgcttctt ccagtccatc cagcaaatca
     1321 gcgataatat ccgcttcccg atgacggatg tgacgcttaa acgcagcaag agccgcatca
     1381 caatcccgtt cagcatttgg gctgtccggg gtagcctgat accacgccag cgtcgattga
     1441 tagttttgtg ctgcctcacg cagtgcctga tagtcaatct tgcccactgg ctgcctcctt
     1501 tgccgggatt tctaactttt gagtggttgt atcaaattca aacaacttaa ccacgtcatc
     1561 aaacaggaca taatcaccat caggatcttc agtcatatca gcgccacaat cctgaccgca
     1621 cgagtcgcaa cctcccatat caagctcgta tcgcttcagg ttcgcgatat ttgataaatt
     1681 cagcgccagt atagccagat cattaacctc gtctgcgata taccctgcac catgtccata
     1741 catttcaatg cgggatatga tttcttctac acgttgtttt gtgatcgtca tttttctctt
     1801 cactccgata tacaaggatt actacacccc ctctgctgat tgcgcgagct ggatcccctg
     1861 gttccatgcc gtcaattccg aaggcttcga aaaatgcatc cattgccttc tggcgttgct
     1921 cctgcttacg gcgtttattc cattttttca tgaacaacag tgacagccat cgtccgctgc
     1981 agaacataat gtagaaataa ccaagaagtg ccaggccggc gttcagggcc atatcaatcg
     2041 ttatcgccgg gtcaatattc actgcccacc tcctgaaaaa tcaccgcatg gcccggtttc
     2101 tcctccagtg ccagctcagc ccctgcacct gccgactgct gccagccttt cagcatgtaa
     2161 accgcatcca cgcaacggag cattgccatg caaatatcca tgtagtgtgg ctgagtcagc
     2221 ccgtccggaa gtactgccgg gtttaaaacg gtatgccctt cccgtttcag cacctcttcc
     2281 gttttgtgaa atgcctcgcg gttaaaattt tcatatcccg tcatcggacc ggcgatataa
     2341 accctcaccc tcactcctga accctcctgt cgaaataaac gtagttattc actgcgccca
     2401 acttcatccc aaacttttcg gcaatttccc gtcggggtac accacgctga tgcagttgcc
     2461 acgccagctc aatatcacgc tgtgaacatt tggctgactg gtgataatca ccccgtaaca
     2521 tcatgctgat acccagttcc cgcgctttcg tcctgacggc tgactcacta cggccaatca
     2581 gataaccgat gctttcgact ctcatcgttc ccgcacactg ccggagtatc aggatttcag
     2641 cccagcgcca cttcttccag ccactcaccg ctgctgctct ctggtggcgg taatatcccg
     2701 gagaatatcc ctgtgtttgt tcagttcccg tagcgcagca cagactcgct cccacttctg
     2761 aacattactt ttcgcccggc gcagctcgcg gttagccaca tgcagcgatg gtagaatcag
     2821 gtcatctgct ttcgcttcag tgaacgattg ctgcaacttc acaatgtcct ccaccgcttc
     2881 tgttttaatt tcttcctgtg cagccgtttc ctggactggt aacgcaacac cagctggctg
     2941 aggaagggct ttaccctccg tttcagctac ggatgctgct ttcggctctg ccggtaaatc
     3001 aacgcccggt atgcagtaac gaaatttacc atcctgattc acgcgaatca gacacccttt
     3061 gctgattgcc attgccagcg atgaattcgc ccggcgggag gtaattccga acatcaatgc
     3121 cagttcatcc gccgtttgtg ggccatgttg ttcaatcgcc tctgtcagca tttgcgccgt
     3181 cactttcggt accggtgaca ccggttcact ttcaccagcc tgagtcagcc accatatcgc
     3241 ccccttgtta tccgcttctc cacggcgttt cagtttccac agttcgttga cagcctcttc
     3301 acgactaatt ccaaggcgag ctgccaccac atgtgaagag gctcttttca gtgctttcag
     3361 tgcgtcagat acggtttcca ttaaaatttc ctccgacaaa atcgtttctc agattcaaac
     3421 aaaaccagcc gccttccggc gctcgtattc ctgtttcagt aattcaattg gcgttggccc
     3481 ttgcgggcac ttcggcgatg cgagttgtct ccggatcggg ggaatactca tcccgttacc
     3541 aacttgcttt gcccatttcg tcagttgccg ttctgcaagt cgttttaact caccttccgt
     3601 catctgccgc tcaatccctc tgtcacgcat ctcgaggcaa atgtggtaca gcaccggctg
     3661 tggccacggg tatttatcac tcccgtcgta ccgccaggat tcattcctcc agcgacggta
     3721 ctcctccatc acggcatcca ccgtcaggcc aaatggattt gccccgctct ccgaaatcaa
     3781 cgccacaaac tcagccag
//
LOCUS       DBFIFI010000291         3782 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.292, whole genome shotgun sequence.
ACCESSION   DBFIFI010000291 DBFIFI010000000
VERSION     DBFIFI010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3782
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.292"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(231..950)
                     /gene="dcuR"
                     /locus_tag="N7B39_004587"
     CDS             complement(231..950)
                     /gene="dcuR"
                     /locus_tag="N7B39_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HGU2424420.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(947..2578)
                     /locus_tag="N7B39_004588"
     CDS             complement(947..2578)
                     /locus_tag="N7B39_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HGU2424421.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            2759..2989
                     /gene="yjdI"
                     /locus_tag="N7B39_004589"
     CDS             2759..2989
                     /gene="yjdI"
                     /locus_tag="N7B39_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HGU2424422.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            3001..3273
                     /locus_tag="N7B39_004590"
     CDS             3001..3273
                     /locus_tag="N7B39_004590"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU2424423.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            3500..>3782
                     /gene="ghoS"
                     /locus_tag="N7B39_004591"
     CDS             3500..>3782
                     /gene="ghoS"
                     /locus_tag="N7B39_004591"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HGU2424424.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFR"
BASE COUNT          994 a          836 c          918 g         1034 t
ORIGIN      
        1 catttgtggg taaaaataca cataatgcgg gtgacataat agttaattaa cttttgttag
       61 cgttttgaaa ttaaaaacac cgttcacctg aagagatatt aatttttagc gatgatggag
      121 ggataattat atttgatctg gcacaagttt tactgatgaa ggatgtaact tgtaccaggg
      181 gtattttgca ttacggtaat aattattact tacagataac cagcgatccg ttattggcaa
      241 tattgtttca gtagtgagta gtgttctgcc tgaatacggt aacggtaaac tggacgcccc
      301 gtgacgccat aatggatact ggtgaacaag atgtggcagt tgaccagcca gataaggtat
      361 ttacggcagg aaacacgcga aatgttaacc tcgttggcta gctcgtcggt tgaaaactca
      421 tagtcctgat gcgcgtcaat ccactggcac agtgtgcgta acgtctgcgg cgttaagcct
      481 tttggcaagc gacgaggatc ctgctcgttg gagctgctgc cgtggattag ctgatcaagc
      541 tcggcctggt cataatactg atgtttttcc agcgccattt tcttttgccg ccagccggtg
      601 agcgcctctt caaagcggga agcctggaag ggtttgatca ggtaatccac gacaccgtaa
      661 tgcagcgaat ctttaatggt tgccgcatcg gctgcggagg agatgacaat cacatcactt
      721 ttgcaacgcg cgttatgcag gacaggcagt aaatcgagcc cgttctcttt ttgcatatag
      781 atatcgagca atatcaggtc gataggcgta tcgctattga agataatctc tttggctttc
      841 tccagcgtcg aggctgttcc acagcattga aagcctggga tttgtgctac gtatcggcga
      901 ttcagctccg cgaccattgc gtcgtcatcg ataattaata cattgatcat ctgttcgacc
      961 tctccccgtc ccagggtatc tggacaaaaa attgtgtgaa aatcccgggt tccgattcca
     1021 cggcgatgct gccgccgaga ttttctacct gttgtttgac aagtgctaaa ccgacgcctc
     1081 gctcgcttcc ttttgtcgag acacctttgt caaaaatgtg atcgattttg tcgggtgcga
     1141 tccccggtcc atcatcgtta acttcgcagt gcagccagcc gtgacggtag tgcaatgtta
     1201 cgctaatttc gccaccgggt tccggcccta atgcttccag cgcgttttct atcagatttc
     1261 ccaatgtggt aatcagcgtc gcgacctggt cctcactgcc gctgtctggc agctggcttt
     1321 cactgtttaa aatcagcgta tggcctaaat cggtcgcgcg gttaatcttg ctgattaaaa
     1381 aaccagcgat aaccggagat ttgatcttac ccagcagaga gccaatctct tcctgatagt
     1441 tattggctgt tttgagaatg taatcttcca actgcttata actcttcaga tgcaataatc
     1501 cgagaatcac atgcaattta ttcataaatt cgtgggatcg ttcacgaagt gcatcagcat
     1561 agttgaccag accgtcgagt cgctgcatca gtttacgaac ttcagttttg tccctgaagg
     1621 ttgaaatggc accgatgata acgccattac tgcgcaccgg aacggtgttg atcagtaata
     1681 gccggtcttt aatcgtaatc tcttcgtcgc ggcgcggcgt accgtcgcgt aacacttccg
     1741 agacatctac cacctgtgac catgagtggc tgagcgtcga cagtttctca tcgtcctgcg
     1801 acttacggta attcagcaat tcttgtgcgg catcgttgat cagcgtgacc tcgccgcgat
     1861 cgtccacggc aacgacgcct tctttgatag actgcaacat ggcctggcgt tgctcaaaca
     1921 gcgtggagat ttcgtagggt tccaggccga aaaggatttt tttcagtacc ttaaccagaa
     1981 tgcaggtgcc aatcagtccg accagcatgc caaataatac cgaccagata atgctccagc
     2041 gactgtcatt gatctgttgg gtcacgcggc ttaactcaag gccgatcgcc accacgccaa
     2101 tttgtttatg attttcatcg tagatggggg taaatacgcg taaagcctgc gccagaaaac
     2161 cgcgattgat agcgacattt tcttcgccat tcagcgcttt aaggatgtca tcacctttaa
     2221 atggctgacc aatacgctgg gcttcaggat gcgagtagcg aagactttgc atatcggtaa
     2281 cgacaataaa cagcagatcg ttgcgtttgc gcacggcttc cgcgatggcc tggatgccgc
     2341 tctcctgcgg ttttttctgc aagccctgac ggatttccgg cgagtcggcg agagtacgcg
     2401 ccactgccag tgccttgttg gctagcccgt ctcgcgtcat atcactgatt tgcgagaagt
     2461 aaatcagatg caccaccaat agcaccgaga acagtaccgc gctgaccatt aagatcactg
     2521 tggtactcaa tttcatcgga cgtttgcgta acatgcggta gggcaatgaa tgtctcatca
     2581 gcttccttgt gtgacaaatt tcttaagcat tatctctgat gaggcgggta attcaaaggg
     2641 agtaagaatg attggctata taggggaaga gactctggca acggaaactg ccagtgctgt
     2701 atgaagattc cagggctatg cttatagtga aaaccatact gatgagagag ggaaggtcat
     2761 ggatcaggcg ctactggacg ggggttatcg ctgttatacc ggcgaaaaga tcgatgtcta
     2821 tttcaacact gcgatatgtc agcattctgg caattgcgta cgtggcaacg gcaagttatt
     2881 taatctcaaa cgaaagccgt ggatcatgcc ggatgaagtc gacgttgcca ctgtggttaa
     2941 agtgattgat acgtgcccga gtggtgcgct gaaataccgt cataaataag cgagggtaaa
     3001 atggaaatac gcgaaggcca caataaattt tacattaatg acgaacaagg caagcaaatc
     3061 gctgaaattg tctttgtgcc gaccggagag aatttagcga ttatcgaaca taccgatgtc
     3121 gatgaaagcc tgaaagggca agggattggt aaacagctgg ttgcgaaagt cgtggaaaaa
     3181 atgcgtcggg aaaaacgaaa aattatccca ttatgtccat ttgcgaaaca cgaatttgat
     3241 aaaacgcggg agtatgatga tattcgcagt tgatggaaga gtacagattc acgatatttt
     3301 tcatgctctc cgcggtgtga tgcaggagag catctgaagg gtagggggat gcacaaagaa
     3361 tgggcagaga gcgcgttttt ttgtcccaag tcatcccctt tactgagcaa aaaaaagaat
     3421 atctcctata tgagaatcat caatcggggt taataaattt tgcgtcccca gagtgttcaa
     3481 tatttatagg agtcatatta tggaaggtaa aaacaagttc aatacttatg ttgtttcttt
     3541 tgattatcca tcatcttatt cctcagtgtt cttaaggtta agatcattga tgtatgatat
     3601 gaatttctct tctatcgtgg ctgatgaata tgggatacca cgacaattga atgaaaattc
     3661 cttcgcaata acgacatcgt tagccgcaag tgaaatcgaa gatttaatca ggcttaaatg
     3721 cttagactta ccggatattg actttgacct caacattatg acagttgatg actatttccg
     3781 tc
//
LOCUS       DBFIFI010000292         3755 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.293, whole genome shotgun sequence.
ACCESSION   DBFIFI010000292 DBFIFI010000000
VERSION     DBFIFI010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.293"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..533
                     /locus_tag="N7B39_004592"
     CDS             <1..533
                     /locus_tag="N7B39_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HGU2424425.1"
                     /translation="FMILKDVNEHMEGLFQRFSKLLPFRIDFAYRKDTPSFGHSCRHS
                     MCIEMHRLLSETQTMLAGYYWVMEYTSNKGLHIHFIGYLDGQRHNKSYRISRQLGDIW
                     RRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAKQDQKEHGIILR
                     RSRLPEKSNRGRPRHN"
     gene            688..3048
                     /locus_tag="N7B39_004593"
     CDS             688..3048
                     /locus_tag="N7B39_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HGU2424426.1"
                     /translation="MVHKSDSDELAALRAENARLVSLLEAHGIEWRRKPQTPVQRVSV
                     LSTDEKVALFRRLFRGRDDVWALRWESKTSGKSGYSPACANEWQARICGKPRIKCGDC
                     AHRQLIPVSDLVIYHHLAGTHTVGMYPLLEDDSCYFLAVDFDEAEWQKDASAFMRSCD
                     ELGVPAALEISRSRQGAHVWIFFASRVSAREARRLGTAIISYTCSRTRQLRLGSYDRL
                     FPNQDTMPKGGFGNLIALPLQKRPRELGGSVFVDMNLQPYPDQWAFLVSVTPMNVQDI
                     EPTILRATGSIHPLDVNFINEEDLGTPWEEKKSSGNRLNISIAEPLKITLANQIYFEK
                     AQLPQVLINRLIRLAAFPNPEFYKAQAMRMSVWNKPRVIGCAENYPQHIALPRGCLDS
                     VLSFLRDNNIAAELIDKRFAGTECNAVFMGNLRAEQEEAVSALLRYDTGVLCAPTAFG
                     KTVTAAAVIAKRKVNTLILVHRTELLKQWQERLAVFLQAGDSIGIIGGGKHKPCGNID
                     IAVVQSISRQGEVEPLVRNYGQIIVDECHHIGAVSFSAILKETNARYLLGLTATPIRR
                     DGLHPIIFMYCGAIRHTAVRPKESPHNLEVLIRSRFTSGHLPSDARIQDIFREIALDH
                     DRTVAIAEEAMKAFGQGRKVLVLTERTDHLDEIASVMNSLKLSPFILHGRLSKKKRAM
                     LISGLNALPPDSPRILLSTGRLIGEGFDHPPLDTLILAMPVSWKGTLQQYAGRLHREH
                     TGKSDVRIIDFVDTAYPVLLRMWDKRQRGYKAMGYRIIADGDESVI"
     gene            complement(3210..3386)
                     /locus_tag="N7B39_004594"
     CDS             complement(3210..3386)
                     /locus_tag="N7B39_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424427.1"
                     /translation="MTEILRIAQTYEPWLDFWELVVQNVSTIASHTATNRPLPEAIMA
                     NYQLDEQLLEGCRP"
     gene            complement(3524..3755)
                     /locus_tag="N7B39_004595"
                     /pseudo
     CDS             complement(3524..3755)
                     /locus_tag="N7B39_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004389801.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT          962 a          784 c          969 g         1040 t
ORIGIN      
        1 acttcatgat tctgaaagac gtgaatgaac acatggaagg tctgtttcaa cgtttttcta
       61 aactgttacc tttcagaatt gattttgcct acaggaaaga cacgcccagt tttggtcatt
      121 cctgtaggca cagtatgtgt attgaaatgc accgcttact tagtgaaacc caaacgatgc
      181 tggcgggata ttactgggta atggaatata cgtcaaacaa gggacttcat attcatttta
      241 tcggctacct ggatgggcaa cgtcacaaca aatcttatcg gatatccagg caactgggag
      301 atatctggag gcggatcacg gaaggggagg gttattttca cctgtgcagg gctaaagaca
      361 aatacccggt gcgtatcgat cacgtaattc actatagtga taaatctgcg gtagacgact
      421 tacgttacgc ccttagctat ctagccaaac aggatcagaa agaacacggt attattctga
      481 ggcgtagcag gcttcccgaa aaaagtaacc ggggccgacc acgacataac tgaattacag
      541 ctgtattagg tattagaatt aagtttctgt aagttgttgg gaggtgcttg agttaatagg
      601 cagaagaggg cggggcgctt tgtcatcagt gtaggggacc cgaatttctg ccgtggtgcc
      661 tcagtggatc ggatacaggt aacgaatatg gttcataaat ctgacagtga tgaattagct
      721 gcattgaggg cagaaaatgc ccgtctggtc tcattacttg aagctcatgg gattgaatgg
      781 cgacgtaaac cgcagactcc tgtgcagcgc gtttctgtat tatccaccga cgagaaggtt
      841 gcattatttc gtcggttgtt tcgtgggcgt gatgatgtat gggcacttag atgggaaagt
      901 aaaaccagcg gcaaatcagg gtactctcct gcctgcgcta acgaatggca ggcgagaata
      961 tgcggtaaac cccggataaa atgtggggac tgcgctcacc gtcagttgat tcctgtatcc
     1021 gatctcgtca tctaccacca tctggccggt acccatactg tcgggatgta tccgttgctg
     1081 gaagatgatt cctgttattt tctggcagtt gattttgatg aagctgaatg gcagaaggat
     1141 gcatccgcat tcatgcgatc ctgtgatgag ctgggtgtac ctgctgcgct ggaaatatcc
     1201 cgttcacgtc agggggcgca tgtctggata ttttttgcct cacgagtttc ggcccgcgaa
     1261 gctcgccgtc tggggactgc tattattagc tatacgtgta gtcggacccg acagctgcga
     1321 ttggggtctt atgaccgatt atttcctaat caggatacta tgccaaaagg gggatttggt
     1381 aatctcatag cgttacctct gcaaaaaaga ccgcgtgaat tggggggaag cgtttttgtt
     1441 gatatgaatc tccagcctta tcctgatcag tgggcttttc ttgtatcggt gaccccgatg
     1501 aatgtgcagg atattgaacc gacgatatta cgggctacag ggagtatcca tcctctggat
     1561 gtgaatttta tcaacgaaga agacctgggt acgccgtggg aagagaaaaa atcatcagga
     1621 aacagactga atatttctat tgcagaaccg ctgaaaatca cgctggcaaa ccagatctat
     1681 ttcgaaaaag cgcaattacc tcaggtgctg attaaccgac ttattcggct ggcagcattt
     1741 ccgaaccctg agttttataa ggctcaggca atgcgtatgt cagtctggaa taagccccgt
     1801 gttataggtt gtgcggagaa ttacccgcaa cacattgcgt tgccccgggg atgtctggac
     1861 agcgtattat ctttccttag ggacaacaat attgctgcag aattaatcga taaacgattt
     1921 gccgggacgg aatgtaatgc cgtttttatg ggaaacctca gagcggagca ggaagaggcc
     1981 gtttcggcat tactccgtta tgacactggt gtgctttgtg cgccaacggc ttttggtaag
     2041 acagttaccg cagcggcagt gattgccaag cggaaagtga atacactgat actggtacac
     2101 cggactgaat tgctgaagca gtggcaggag cgtctcgcgg tgtttctgca ggccggtgac
     2161 agtattggta ttatcggggg aggtaaacat aaaccctgtg gcaatattga tattgcggtg
     2221 gtgcagtcca tatccagaca aggagaagtt gaacctctgg tcaggaatta tgggcaaatc
     2281 attgtggatg agtgccatca tattggcgcg gtttcatttt ctgcgattct gaaggaaacg
     2341 aatgccagat atctgcttgg cctgacggca acaccaatcc gacgggatgg tctgcatccc
     2401 attattttta tgtactgtgg tgccattcgc catacagcgg tccgcccgaa ggaaagccca
     2461 cataatctgg aggtactgat ccgttcccgt tttacatctg gtcatttacc atcggatgcg
     2521 agaatccagg atattttcag agaaattgct ctggatcatg acagaacggt ggcgatagct
     2581 gaagaagcca tgaaagcttt cgggcagggg cgaaaagttc tggtactgac tgaacgtaca
     2641 gatcatctgg atgagatagc atcagtgatg aattcactga aattgtctcc ctttattctc
     2701 catggtcgac tatcgaaaaa aaagcgtgcg atgctgatat ccgggctgaa tgctcttcct
     2761 cccgattctc ctcgaatttt gttgtcaaca ggcagactta ttggtgaggg atttgaccac
     2821 cctccgctgg atacgctgat tcttgccatg cctgtgtcat ggaaagggac attacagcag
     2881 tatgcagggc gtcttcacag agagcatacc ggtaaaagcg atgtcaggat cattgatttt
     2941 gtggataccg cgtatcctgt gctgctcaga atgtgggata aacgtcagcg gggttataaa
     3001 gcgatggggt acaggattat agctgacggc gatgaatcag tgatttaatt ttaaggagta
     3061 ctttaccctt tttaaataat ttgagctccc cgggttgttc gggcaacaaa gagtcccagt
     3121 atacaggctt caggaatatg ttgtagtatc aggcttttca tcgctttaaa atgactaccg
     3181 gcagtgagaa cgtcatcaaa aataacaatc tagggcctgc acccttccag taattgttca
     3241 tccagttggt agtttgccat aatcgcttct ggtaagggcc ggtttgttgc ggtatgagag
     3301 gctattgttg atacgttttg cacaaccagt tcccagaaat ccagccatgg ttcgtaggtt
     3361 tgcgcaattc gcagaatttc tgtcatacgg tcatcgtaag cggcatctgt acggcatttg
     3421 gatggtggta cgggaacaaa tgtgtattcc tcaagattac cgatatttga tatgagcatc
     3481 tgagcaacat actcaatagc agccttttta tagtggtaat caggctacga aagtacagcg
     3541 ttttataaat tgtctcaggt gatattcgca gcgttttttg acgtggtttt gttcgcctta
     3601 accatcctga tatttgctct ggagaccatt tcatctccag cttttccaga acaagctttc
     3661 gcaatggtaa attttgatcc agtaagcacg gttttggcct tttcgccatt ctgttggctc
     3721 ggttattagc atcaacagct ttgtaatagc gtctg
//
LOCUS       DBFIFI010000293         3751 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.294, whole genome shotgun sequence.
ACCESSION   DBFIFI010000293 DBFIFI010000000
VERSION     DBFIFI010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.294"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(115..1464)
                     /gene="lysC"
                     /locus_tag="N7B39_004596"
     CDS             complement(115..1464)
                     /gene="lysC"
                     /locus_tag="N7B39_004596"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HGU2424428.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     regulatory      complement(1556..1752)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            1989..3638
                     /gene="pgi"
                     /locus_tag="N7B39_004597"
     CDS             1989..3638
                     /gene="pgi"
                     /locus_tag="N7B39_004597"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HGU2424429.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
BASE COUNT          938 a         1002 c          961 g          850 t
ORIGIN      
        1 gcaattattc tccgataaag acgctggtta acagagtaca ggctcgttta ttgatgagca
       61 tagtgacaag aaaatcaata cggcccgaaa tatagcttcc aggccataca gtatttactc
      121 aaacaaatta ctatgcagtt tttgcaccac ctgctcggca tcttcgccgg gcaccaggaa
      181 gcacaggtta tggctggatg cgccgtaaca aatcatgcga atgttgaacg gttccagtac
      241 gccgaatacc tctttgccaa cgccgcaggc ttttgacagg tcattgccaa tcaacgcgac
      301 cagcgccaga ccttcttcca cctccacccg gcacagtgcg gaaagctcca tcagcagaga
      361 ttgcgtcagc aacgtatcgc cagtggaggt tgaaccggtg gtatcaaggg ttaatgccac
      421 gctcacttct gacgtggtga ttaagtctac cgaaatatta tgccgcgcga ggatgccgaa
      481 aacttccgcg aggaaaccgc gagaatgcag catattcagg ctgtgcaaag tgagcagagt
      541 ctgattgcga cgaagcgcca gcgcgcggaa cagcggcgga ttttcagttt tattgcacac
      601 cagcgtacca cctgcgcgtg ggtctttgct ggagccgaca aagactggga tatcgctgcg
      661 tactgcgggt agcaacgttg ccggatgcag tacttttgca ccaaaagttg ccatctctgc
      721 cgcttcggca aacgcgattt catcaatgcg ttttgctgcg gaaactacgc gtggatcggt
      781 ggtgtagatg cccgggacgt cggtccagat atcaacacga gatgcgtgta aagcctccgc
      841 cagcaaggct gccgtataat cgctgcctcc acggccaagc gtcgttgtac gacctttatt
      901 ttcgctaccg ataaatccct gggtgatcac taagccttca ttgagacgtg ggagcagctg
      961 cagcgcggcc agttccgcca gcgcggctac atctggctct gcacgaccaa atcggtcgtt
     1021 ggtacgcatc actttacgta catcaaacca ctgtgcctga acatcgcgtt cgcgcaggat
     1081 ctcgacaaac agcagggtcg acatcagctc gccgtggctg accagttcat ctgtcagcgc
     1141 cggagacgtt gccagcgccg ccgcttctgc cagaacagta atgttctcca gcagacgttc
     1201 aatctcttca cggataacgt tcgggtaacg cagacgttcc agaatggcaa actggatgtt
     1261 gcgaatagcg tcgagttttt cgaatcgctc gccaggttcc agtccttccg ctaaagcgac
     1321 cagcagatta gtgataccag cagaagccga gaggacaact aaacgcacgt tggcatcaga
     1381 aagcacaata tcagcgctgc ggttcatggc gtcaaaatca gctacgctgg taccgccaaa
     1441 tttggagaca acaatttcag acataactac ctcgtgtcag gggatccatt ttcagccttg
     1501 gcacaaggga agagcggaag acgggtgggc gcagagcgat acttcgctac tattttcacc
     1561 cagaagtgct ccaccacttg cgaaacgccc gactgcgaac gcttctggtg acaacccagg
     1621 ggattcagcc cctgtagccg atgatgaacg tggccagccg ttcaatcacc tcggcgatgc
     1681 accccctcag gtgttatcac aggactggct cctccaacac cgttacttgg gcaacgcgcc
     1741 tcttctggcc tgcgctagcg caggtagtac atttataaat aaagggtgag cggggcggtt
     1801 gtcaacgacg gggtcatgcg gatttttcat ccactcctgg cggtcagtag ttcagctaat
     1861 aaatgcttca ctgcgctaag ggtttacact caacattacg ctaacggcac taaaaccatc
     1921 acatttttct gtgactggcg ctacaatctt ccaaagtcac aattctcaaa atcagaagag
     1981 tattgctaat gaaaaacatc aatccaacgc agaccgctgc ctggcaggca ctacagaaac
     2041 acttcgatga aatgaaagac gttacgatcg ccgatctttt tgctaaagac ggcgatcgtt
     2101 tttctaagtt ctccgcaacc ttcgacgatc agatgctggt ggattactcc aaaaaccgca
     2161 tcactgaaga gacgctggcg aaattacaag atctggcgaa agagtgcgat ctggcgggcg
     2221 cgattaagtc gatgttctct ggcgagaaga tcaaccgcac tgaaaaccgc gccgtgctgc
     2281 acgtagcgct gcgtaatcgt agcaataccc cgattctggt tgatggcaaa gatgttatgc
     2341 cagaagtcaa cgcggtgctg gagaagatga aaaccttctc ggaagcgatt atctccggtg
     2401 agtggaaggg ttataccggc aaagcaatca ctgacgtagt gaacatcggg atcggcggtt
     2461 ctgacctcgg cccgtacatg gtgaccgaag cactgcgtcc gtacaaaaat cacctgaaca
     2521 tgcactttgt ttctaacgtc gatgggactc acatcgctga agtgctgaaa aaagtaaacc
     2581 cggaaaccac gctgttcctg gtagcatcta aaaccttcac cactcaggaa accatgacca
     2641 acgcccatag cgcgcgtgac tggttcctga aagcggcagg tgatgaaaaa cacgttgcga
     2701 aacactttgc ggcgctctca accaatgcca aagccgttgg cgagtttggt attgatactg
     2761 ccaacatgtt cgagttctgg gactgggtcg gcggtcgtta ctctttgtgg tcagcgattg
     2821 gcctgtcgat tgttctctcc atcggctttg ataacttcgt tgaactgctt tctggcgcac
     2881 acgcgatgga caagcatttc tccaccacgc ctgccgagaa aaacctgcct gtactgttgg
     2941 cgctgattgg catctggtac aacaatttct ttggtgcgga aactgaagcg attctgccgt
     3001 atgaccagta tatgcaccgt ttcgcggcgt acttccagca gggcaatatg gagtccaacg
     3061 gtaagtatgt tgaccgtaac ggtaacgttg tggattacca gactggcccg attatctggg
     3121 gtgaaccagg cactaacggt cagcacgcgt tctaccagct gatccaccag ggaaccaaaa
     3181 tggtaccgtg cgatttcatc gctccggcta tcacccataa cccgctctct gatcaccacc
     3241 agaaactgct gtctaacttc ttcgcccaga ccgaagcgct ggcgtttggt aaatcccgcg
     3301 aagtggttga gcaggaatat cgcgatcagg gtaaagatcc ggcaacgctt gactacgtgg
     3361 tgccgttcaa agtgttcgaa ggtaaccgcc cgaccaactc tatcctgttg cgcgaaatca
     3421 ctccgttcag cctgggtgcg ttgattgcgc tgtatgagca caaaatcttt actcagggcg
     3481 tgatcctgaa catcttcacc ttcgaccagt ggggcgtgga actgggtaaa cagctggcga
     3541 accgtattct gccagagctg aaagatgata aagaaatcag cagccacgat agctcgacca
     3601 atggtctgat taaccgctat aaagcgtggc gcggttaatc atcgttcatg cctgatgccg
     3661 ctatgtaggc cggataaggc gtttacgccg catccggcaa ctggtgcctg atgcgacgct
     3721 ttacgcgtct tatcaggcct acaggtcgat g
//
LOCUS       DBFIFI010000294         3745 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.295, whole genome shotgun sequence.
ACCESSION   DBFIFI010000294 DBFIFI010000000
VERSION     DBFIFI010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3745
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.295"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..746
                     /locus_tag="N7B39_004598"
     CDS             <1..746
                     /locus_tag="N7B39_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2424430.1"
                     /translation="TIGKSSNAQAVLPYVVKIARGTPDFTGERKSTWFISDTVIANIG
                     GESSSSIDFWLGICKALKFNWCVNYLTSKLAGDTFTLGLNISYYPKNTTCKPENTVIK
                     VDDIALFQLRNQGKIAANSKEGTITLKCDNLFGDKKQASRNMVVYLSSSDLVKGSNTI
                     LRGKTDNGVGFVLDLTEPPKGTEAAIKISANGDQGAATSLWKTDKPGVSLNSNIINIP
                     VMASYYVYDEKKVKSGALEATALINVKYD"
     gene            complement(828..1166)
                     /gene="rcnB"
                     /locus_tag="N7B39_004599"
     CDS             complement(828..1166)
                     /gene="rcnB"
                     /locus_tag="N7B39_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HGU2424431.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHYKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(1384..2241)
                     /locus_tag="N7B39_004600"
     CDS             complement(1384..2241)
                     /locus_tag="N7B39_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HGU2424432.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIISTA
                     FWMFWRTWRGERNWLENMHEHHHHDHEHHQDHDHDHDHDHDHDHEHHHHHEHGDNEEY
                     QDAHARAHANDIKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGA
                     TLVVSFSIGLALTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMG
                     VHGFMGIMR"
     gene            2362..2634
                     /gene="rcnR"
                     /locus_tag="N7B39_004601"
     CDS             2362..2634
                     /gene="rcnR"
                     /locus_tag="N7B39_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HGU2424433.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            complement(2744..3058)
                     /locus_tag="N7B39_004602"
     CDS             complement(2744..3058)
                     /locus_tag="N7B39_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein"
                     /protein_id="HGU2424434.1"
                     /translation="MKKLLVTVIFVAMMPNAFALSHGDSATLKEGTFNCKKLTDFYEM
                     ISYIQDNDQQAMLSLITSNKCRVLDESMTVEIQSVDDKGFVSFITPGGHGGWAVKQFF
                     EN"
     gene            complement(3068..3415)
                     /locus_tag="N7B39_004603"
     CDS             complement(3068..3415)
                     /locus_tag="N7B39_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424435.1"
                     /translation="MKSKIFAALLLLPLSTYASSYKCDALQQGQIGSNGKTVTAQSSI
                     DIDGKQMHFSLNGVSWDLSYIGEKQIAKMYSTESGNVSVSFIDANPIIFVLHLVNDDG
                     RKGTYNISECSEL"
BASE COUNT         1061 a          768 c          852 g         1064 t
ORIGIN      
        1 acacaattgg gaaatccagc aatgcgcagg cagtattacc ttatgtggtt aaaatagcca
       61 gaggcacacc tgattttact ggagaaagaa aatctacctg gtttatttca gataccgtga
      121 ttgcaaatat tggcggtgag tcatcgtcat ccatcgattt ttggttgggt atttgtaagg
      181 cattgaagtt taactggtgt gtgaattatc tcaccagcaa actggcgggg gatacattta
      241 cgcttgggtt aaatatttcc tattatccta aaaatacgac ctgtaagcct gaaaacaccg
      301 ttataaaagt agatgatatc gccttgttcc agctcagaaa tcagggaaag attgcggcga
      361 acagtaagga aggaacaatt acgttgaaat gtgataatct tttcggcgac aaaaaacaag
      421 catcgcggaa tatggttgta tatctttcta gcagtgactt agttaaagga agtaatacta
      481 ttttgcgtgg taaaacagat aatggtgtag ggtttgtgtt ggatctaaca gaaccaccaa
      541 aagggactga ggctgccatt aaaatttcgg ccaacggcga tcagggcgcg gcgacatcat
      601 tatggaaaac agataaacca ggagtttcat taaatagcaa cattattaat ataccagtca
      661 tggccagtta ctatgtatat gatgaaaaaa aagttaaatc tggcgcactg gaagcaaccg
      721 cattaatcaa cgtgaaatac gattaataac aagcaagatg agcaagtggc taataataag
      781 aaataataag gtgtctgcat ttccccctct ctccgagggg gcttttttta gcgatgataa
      841 aaaatctcac cgtcgtaggc tttaatgatt ttaccgtcgg tgtcgctgat caacacgtac
      901 gcgccaccca tataggtcca gtgcgtcccg gcatccggcg cgggcaggtt acgcaactgc
      961 cactgcttaa tgttgtactc atcggtacgg tacatctctg gtacggtgtc ccctggtttg
     1021 taatgcttaa agtcagcaat gaaactgctt aactcatact tagaaattcc cgaggtattg
     1081 gtcgaacccg cggtggctgg tgcggcccag gcggcactgg taaccagcaa aagcgcaccc
     1141 agcaacatct tatttttaat ggtcataaac tctccacttt ggggatctgt aatatcagtc
     1201 gcatatcttt cctgccatac aatataactg acaagcgata ttttgttagt gatcccatga
     1261 tttcgtaaaa agtgtaaatg aaaggatgta gcaaaagcat cctcattgag cgagaagtga
     1321 gcggaataag ctgatttaag aatcatcctg ggctaaaagg atagcgaagc ccaggtgatt
     1381 gttttatctc ataataccca taaagccatg cactcccata tacaccccga ctaaaccaat
     1441 taacagactg gaaaaatagg gggcgcgttt agcgagagtg ttaaatccgc tccagcgttt
     1501 tgcaacttgt tgaacactga ttgccgctcc aacgcctact gtaaccagcg ttagtgccag
     1561 accgatgctg aaactgacga ccagtgttgc gcccagtgtc agggctttca actgaatgca
     1621 aatcaacagc acggtaattg ctgccgggca gggaataagg ccacctgtta agccaaataa
     1681 caaaatttgc cagttggtga cctctctacc atcaaagcgt cgtttaatgt cattggcatg
     1741 ggctcgtgca tgggcatcct gatactcttc gttgtcgcca tgttcatgat ggtgatgatg
     1801 ttcgtggtcg tggtcgtggt cgtggtcgtg gtcgtggtcg tggtcttgat gatgttcgtg
     1861 atcgtggtga tgatgctcat gcatattctc cagccagttg cgttcgccgc gccaggtacg
     1921 ccagaacatc cagaaagcgg tgctaataat gatcactgcg gaaatcaact ggagccacgg
     1981 ttctgctgat tgagcagtaa agcgcttgct gatcaccatc ccgccaaagg caatcaacca
     2041 gaccactgcg gtatgcgaaa tggttgctgc cagtccgagc atcaccgcct gtttaatggt
     2101 gcctttgatg gcgatgataa acgccgccat catcgttttt gagtgccctg gttccaggcc
     2161 atgaagcgca ccaagtaaga tggcgctggg gatgaagaac caggcgtttc cttgctgaag
     2221 aagagttgta aattcggtca tgataataat tcttagtatt aattcggcaa tctgattcta
     2281 ctccccccca gtacctgata ctacccccca gtagattaat agtgctatga tttttcatgt
     2341 tcttgttaac caggtgttgc catgtctcat acaatccgtg ataaacagaa actgaaagcg
     2401 cgtgccagta agattcaggg ccaggtcgtg gcgctcaaga aaatgctcga cgagccgcac
     2461 gaatgcgctg cagttttaca acagattgct gctatccgtg gcgcggtaaa cggtctgatg
     2521 cgggaagtga ttaaaggtca tctaacggaa catatcgttc accaggggga tgagctaaaa
     2581 cgtgaagaag atctggatgt tgttctgaag gtgctggatt cttatatcaa atgaaagtgc
     2641 ctaatatggc cgtagttgag gcaataaata gagggacaag tgacgttaag ctgaattaga
     2701 gtaaatggcg tgaggacaca cgccattcga ggttatagtt tgattaattt tcgaaaaatt
     2761 gcttcacagc ccagccacca tgaccacctg gagttatgaa tgaaacaaaa cctttgtcat
     2821 ctacgctctg gatttctacc gtcattgatt catctaacac gcgacattta ttggatgtaa
     2881 tgagactcag catagcctgt tggtcattgt cctggatgta actgatcatc tcgtaaaagt
     2941 cagttaactt tttacagtta aatgtacctt ctttaagtgt tgcgctgtct ccgtgactta
     3001 gtgcgaatgc atttggcatc attgccacaa agatgacggt aacgagtagt tttttcattt
     3061 tagcctctta taattcagaa cattcactga tgttgtaagt gccttttcta ccatcatcgt
     3121 ttacgagatg tagcacgaag atgatgggat tggcatcaat gaaagataca gagacgttcc
     3181 cgctttcggt gctatacatc tttgcaatct gtttttcacc aatatatgag agatcccaac
     3241 tcactccgtt caagctaaaa tgcatctgtt tgccgtcaat atcgatggag gattgtgccg
     3301 taacggtttt cccattgctg ccaatttgac cctgctgaag agcatcacat ttataggagc
     3361 ttgcataagt agatagaggt agtagcagaa gtgcagcaaa aattttactc ttcatatttt
     3421 ccctttgctt ggatttggat tacctatttt ggggtagtca ttataaaact attaattagt
     3481 aaaacgaaca agttttcatt tgtttagcaa tggtattgtt taggtttgaa attattacgt
     3541 attttcatag caataggttg gataaacagc agttcaggga acaagttgca ctatctacat
     3601 acttttccgc tttccattgc atgttaggta atagatagcc tttgcccgta agactaaaaa
     3661 tattagaaat gactcattcg aataccttct attcacctta atataggttt gattattgta
     3721 atcgtttaat gtgtcttatg atttt
//
LOCUS       DBFIFI010000295         3716 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.296, whole genome shotgun sequence.
ACCESSION   DBFIFI010000295 DBFIFI010000000
VERSION     DBFIFI010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3716
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.296"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..1059)
                     /gene="yhdY"
                     /locus_tag="N7B39_004604"
     CDS             complement(<1..1059)
                     /gene="yhdY"
                     /locus_tag="N7B39_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGU2424436.1"
                     /translation="MTKVLLSHPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFALERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLLRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYS"
     gene            complement(1069..2250)
                     /gene="yhdX"
                     /locus_tag="N7B39_004605"
     CDS             complement(1069..2250)
                     /gene="yhdX"
                     /locus_tag="N7B39_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGU2424437.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLNNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            complement(2318..3343)
                     /locus_tag="N7B39_004606"
     CDS             complement(2318..3343)
                     /locus_tag="N7B39_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU2424438.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
BASE COUNT         1048 a          950 c          905 g          813 t
ORIGIN      
        1 gctatagcgc gacatgctga aacagaagat ccagtagatc agcgcggcga aaacataccc
       61 ttccgtcgac atacccaacc aggcgggatc aacggttgct tgctgaacgc tactgaaaag
      121 atcgaacaac ccgataatga tcaccaggct ggtatctttg aagagtgcga tgatggtatt
      181 taccagccca ggaattacca gcttcaacgc ctgtggcaga ataaccagcc cctgagtttt
      241 ccagtaaccc aacgccagcg actctgccgc ttcatattgc cctttaggca gcgcctgtaa
      301 tccacctcgc acgacttccg caacatatgc tgactgaaac aggatcacgc caaccagcgc
      361 gcggagcagt ttgtcgatac tggtgccttc tgccataaac aaaggcagca tgaccgaaga
      421 cataaacaga acggtaatca gcggcacgcc gcgccagaat tcgataaaaa tgaccgataa
      481 gatgcgcaca atcggcatat gggagcggcg acctaacgcc agtaagatcc cccacggtag
      541 cgcccccgca atcccaactg atgcaataat taaagttagc gtcagcccgc cccattgccg
      601 ggtttcaacc cgctcaagag caaaaaaacc gccatacatc agccaccaga caatcagtgg
      661 gtaaatcact gcccagaccg caatatagcg accgcgatgc gggagtattt tccagaacat
      721 tggtgcgatt gacacaaggc caatcagtaa tgccaagtta attcgccagc gttggtcgtg
      781 tgggtaaagt ccatacataa actgaccaaa tcgttcgtgg atgaagaccc aacaggcacc
      841 ggcttttgtg cagtcggcac gcgttgagcc aacccagtta gcctgcaaaa atgcccagtt
      901 cagcaacggt gggatcagtt cccacatcaa ccatatgcag ccaatagtca gcaggctatt
      961 gctccagctg gagaacagat tttttcgcac ccacaccatc gcgcggcttg agttatggct
     1021 cgccgggcgc gagggatgag acagcaatac ttttgtcatc atgactcctt agcgttccac
     1081 gatcgcgatg cggcggttat agatattcat cagcagcgag atagtcaggc tgataatcag
     1141 atagaccgac atggtcatgg ctatcgtctc gatggcttgc cccgtctggt tcagcacggt
     1201 gccagcaaac agcgaaacca tatcgggata gccaatagcg gcggcaagag aggagttttt
     1261 gacgatgttg agatactggc tggttaacgg tggaataatc acccgcagtg cctggggaat
     1321 aatgacctgg cgtagcgtaa ccgggttggg taatcccagt gaccgagccg cttcatgttg
     1381 accataaggc actgcctgga tccccgcgcg gataatctcg gcgataaatg cagaggtata
     1441 aaccgaaagt gccagcgtta aggctgccag ttcaggaatt aaaaccatcc cgccgcggaa
     1501 gttaaagcct tgtagggctg ggacatccca gtgtagtgct gcgccaaaaa gccattgcgc
     1561 cagtaaaggc aaaccgatga tcaacactgc ggcgatgggc caggtgcggc gcagctgtcc
     1621 ggtctttatc tggtatgttt tattaaagcg gaatagccca acagaaagga ctatagccat
     1681 aacaacagcc aggataaacg caataaatcc gtctcccagc tgcggtgacg gaatataaag
     1741 cccacgattg ctcaaaaagg ccagatcaaa cgcgctcaca gcctggcgtg gtccgggcaa
     1801 attgcgcaac acggcaaagt accagaagaa gatttgcagc agcgggggaa tattacggaa
     1861 gatctcaata taaattgtgg aaagctttcg tagcagccag ttatccgaaa gtctcgccag
     1921 accgataaag aagcccaaaa cagaagcgaa cacaatacac aatgcagaaa ccagtagcgt
     1981 attgagtaag ccgacaataa aaacgcgtcc gtaggtgtcg ccctgctggt aatcgatcaa
     2041 atgctggaca ataccgaagc cagcgccgcg atccagaaag gcaaaacctg aagtaatgcc
     2101 acgattattg agattcgtta cagtgttgtg aaataaccaa ccaacaatgc cgacaacagc
     2161 aacaacggca aggatctgga ataaccaggc gcgaaccgta gggttggcaa aagagagtga
     2221 gcctttaacg gttgagcggc gatgagacat agaaacctcg gaaagcataa cgctgaagag
     2281 agcgccgccg tagcgacgct cgttacctca caccgactta acgcacgggc ggtgcgtact
     2341 gaataccgcc gttattccag agattatttt gcccacgttt aattttcagc gggctttctg
     2401 aacctacgtt acgctcgaaa atttccgagt agttacccac ctgtttgatg atgttatagg
     2461 cccatttatt atccaacttc agatccttgc cgtaatcgcc ttctttaccc agcagatgtg
     2521 ccatatcagg cgttgctgga ttagccgctt tttcatcgac gttctgggaa ttgatgccca
     2581 tctcttcagc atttagcatg gcgaaaagcg tccagcgtac aatcgagaac cattcatcat
     2641 cgccacgacg aactaccggc ccaagcggtt ctttagagat aacctccggt aagacaatcc
     2701 attcagccgg gttgcttaat ttgatgcgca gggcatacag ttgggattga tccgaagcca
     2761 gcgtatcgca gcgaccggat tccagtgcct tcgctgattc gtcagaacga tcgaaagtca
     2821 ctggtgtgta cttcatattg tttgccttga aatagtcggc gacgttgagt tcggtatcag
     2881 tacccgcctg aatacagacg gtagcgccat ccagttcttt cgcgcttttt agccccgctt
     2941 tatcgtgcgt caggaagcca atgccgtcgt aataagtgac gccagtaaat gccattccca
     3001 tcccggcatc gcgagatgaa gtccaggtcg tattacggga gagcaaatcc acttcccctg
     3061 actgtaaagc cgtgaagcgt tcttttgctg tgagtggggt atatttcact ttcgtgtcgt
     3121 caccaaatac agcagcggca acaccacgac aaacatcaac atcaatacct gaaaacttac
     3181 cgtctgcatc ggcataagag aacccaggta atccatcact gatcccgcat tgcacaaaac
     3241 cttttttctg aacggcatca agcgtcgcgc cagcatgcgc ctgatttgca acggcaagca
     3301 gcacgctggc ggcagccagt gtggctatca tcatcttttt cataatgctt cctgtgaggc
     3361 gaaaattatc gttataggac gtttgggaaa cgtcttttgc ctgtctgtgg ccatcctctt
     3421 ataagcaaaa gtagtgccag cttttgcgca cgatttcgcg tcgcaataag ctgagtgtag
     3481 acaggaaata ataaaaacag cgccccgcaa tggtgcgaaa atgaaacatg cgccatattt
     3541 tggcgcaaaa catttacgcg tgttttacta agcagaataa tgattgttag cgtgagtata
     3601 tttctggtgg gaagtgcgaa agttaaattg atgcgttatt tcgaaagcag cgaaataaca
     3661 cgatataaca tgctaaatca gcagagaatt agccagcaga atatagcaat atttta
//
LOCUS       DBFIFI010000296         3659 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.297, whole genome shotgun sequence.
ACCESSION   DBFIFI010000296 DBFIFI010000000
VERSION     DBFIFI010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.297"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..621
                     /locus_tag="N7B39_004607"
     CDS             <1..621
                     /locus_tag="N7B39_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HGU2424439.1"
                     /translation="PKMYTGIKEFDLSSSWGINNRDDLIQTIYQMTDDGHANDLAGLY
                     LTWHRSSPEEWKALIAGGSERGLIYTQFVAQTAMCCGEGGIKAWDYVRMGFLSRVGVL
                     NKWLTEDESLWLQSRVYERAHHYYHSWMHYFSAYSLGRLYWQSSQCEDNASLREALTL
                     YKYDSAGSRMFEELAAGSDRFYATLPWQPLTVQPECPVTLKDVSDL"
     gene            618..2069
                     /gene="djlC"
                     /locus_tag="N7B39_004608"
                     /pseudo
     CDS             618..2069
                     /gene="djlC"
                     /locus_tag="N7B39_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
     gene            complement(2129..>3659)
                     /gene="hscC"
                     /locus_tag="N7B39_004609"
     CDS             complement(2129..>3659)
                     /gene="hscC"
                     /locus_tag="N7B39_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HGU2424440.1"
                     /translation="NNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNA
                     PELSSLVLRSLKEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLIN
                     EPTAAAMAYGLHTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFT
                     HMLVDEVLKRADVARTTLNESELAALYACVEAAKCSNQSPIHIRWQYQDETRECEFYE
                     NELEDLWLPLLNRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKL
                     PYQSYDPSTIVALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPII
                     ERNTTVPVSRVETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKRTGAYQSI
                     DIRFSYDINGLLEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDML
                     INRTFKAKLEELWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRQASVYLAIEI
                     P"
BASE COUNT          928 a          875 c          929 g          927 t
ORIGIN      
        1 ccaaaaatgt ataccgggat aaaagaattc gatctctcat catcctgggg aataaataat
       61 cgtgatgatt taattcagac tatttatcag atgactgatg atggtcatgc caatgattta
      121 gctggcttat atctgacatg gcatcgctct tcccctgaag agtggaaagc attaattgct
      181 ggtggctcgg agagagggtt gatttatacc caattcgttg cccaaaccgc tatgtgttgt
      241 ggggaagggg gaatcaaagc ttgggattat gttcgaatgg ggtttctgtc acgggtcggt
      301 gtgctcaaca agtggctgac ggaagacgag agtttatggc tgcaatcgcg cgtctatgaa
      361 cgagctcacc attactatca cagctggatg cactattttt ccgcctattc actagggcgt
      421 ctctactggc aatcttctca atgcgaagac aacgcttcgc tacgcgaggc gctaaccctg
      481 tacaaatacg atagcgccgg gagtcggatg ttcgaagagc tggctgcggg aagcgatcgt
      541 ttctatgcca cgcttccctg gcagccatta actgtccagc ctgaatgccc ggtaacgctt
      601 aaggatgtga gcgacctatg aaaacatgtt ggcaaatcct cgaaattgaa agcacgacgc
      661 aaatagacat tatccgccag gcttatcttg ctcgcttacc gttatgtcat cccgaaaccg
      721 atccgcaagg gtttaaagca ttacgccagg cctatgaaga ggccctgcga ctggcggtaa
      781 atcctgtcga ggaagcagat gatgaagaaa aagatgatgc cactgagcat gaaatactac
      841 gtgtattcag ggcattactg gattcagaaa gtgatcgttt tcagccctcc gcctggcaga
      901 aatttattca gcaattaaat acctggaaca tggaggatgt cgatcaatta cgctggccgc
      961 tgtgtgcaat cgctatagaa gcgcgatatc tttcattaaa ttgtgcttct ttgctggcag
     1021 agcgtttgaa ctggcattcg tttaatgaca gcgaaggaat agatgagtaa gaaagggagg
     1081 cttttcttga ggccattcag gctggtgatt gtttcgattt ccttggcctt ctggtatatc
     1141 ccgttgcgtt gcagaaccag actgttgagt attacttcgc gctggaacgt tgctgccgtt
     1201 accatcctga ctatgtcact gcgtttttgg cgatggaagg tccgtggttc attcctgatg
     1261 atgcaaagtt acatcgcaaa ctgttgcgct ggtacagctc ggtgcaaaca ggtatggcgg
     1321 aactcattcc tgtagccgaa cagtggcaaa tggaagaacc agaaagcgaa gatgcccggt
     1381 attaccagtg tgcacaacgt ttgtactgcg gcgaggggga aagcctgctt accgatctct
     1441 gcgcgtactg gggaagttac ccatctacac aagccgataa tttgttgttg cagtggagcg
     1501 cacaacattg cccggattat ttcgcgttat tagtgatggt tatcgaagcg cggagcatgg
     1561 tagatgcgca aggtaaaccg ttgaaatatg ttcctggtga gagcgcccgg acgcgtctgt
     1621 tatgggcgga aattttacat agcggaaaac tatcgccgtt aggtcaatcg tttattgagt
     1681 cgctattctt caaacgcaaa gcatgggcgt ggtggaaatc gagagtcggt agcgagacag
     1741 aagaagattc accgttactg gatttgtatc gggtagcgga acaggtagta cttgaagcgt
     1801 ttccgaaaca agagatgctg gcccgtctta atacaaggct ggaaggcgga gatgctcatc
     1861 cattagaggc cattatcacc cggatgctgt tgacgaaagt aaaactcgag ccggaggatg
     1921 aagatgtcga tgagccaaca cctgaaaacc atgaagaaaa aaatgatgag gatgaaaaac
     1981 cacagagcat taccagcatt atcaaaatca gcttaacggt gctggtgata ggttatgctc
     2041 tcggcaaaat cgcgatgttg tttagttgac gatgtgtttt ttgcgccgat gccggatgca
     2101 gcatttacgc cgcatccggc agccgtgatt atgggatctc aatggctaaa taaacgctcg
     2161 cctgccgcct gacctcatcg acgcgggcca tatcgtttga ctgcagcgcc gcatcaaaat
     2221 cggtgatcac ccggccaatc tcttctcgct cgtcacccag cgcccgcgcc cacaactctt
     2281 ccagtttggc cttaaaggtg cggttgatga gcatatcgcg cggataaatt ttcaatgcgg
     2341 ataaccgcgt ccgactctct tcaatctgct gcgcgctcaa tgttaccggg ctgtggttaa
     2401 tcactctgga cttaacgctg ccgtcttcca gaagcacgtc aacttcaagc aaaccgttga
     2461 tatcataact aaagcgaata tcaatcgact gataagcccc ggttctcttc aacggcacat
     2521 cgaaggattc caccagaatg ttgtttttaa ctttgtggtt ttctccctga tagacgttaa
     2581 ccgtaataga atcctgttcc gggtgcatgg ttgaataagt ttctacacgc gacacgggca
     2641 cagtggtgtt tcgttcaata atcggcgaga aaatgccgga aacgccctgg cggttaacct
     2701 caacgcccaa cgagtaaggg caaatatcag tgaggatcac ttcttcaata tcttcactgc
     2761 gtaagcggca ggctgcctgg attgctgcgc ccagcgcgac aatggtgctc ggatcgtaac
     2821 tttgatacgg taatttgcca aacagacgca cggcgattcg ctgcaccagc ggcatttgtg
     2881 acgcgccgcc aaccagcacc agactgtcga tttgactcgg cttcagacgc gcatcgcgca
     2941 acgcctgttc aatcggcact cgcaagcgat tgagcagcgg caaccacaaa tcttccagtt
     3001 cgttctcgta aaattcgcat tcccgcgttt catcctggta ctgccagcga atgtgtattg
     3061 gcgattgatt gctacatttt gccgcttcca cacaggcata caaggctgcc agttcactct
     3121 cgttaagcgt ggtcctggcg acatccgcgc gttttaaaac ctcatcgacc agcatatggg
     3181 taaaatcttc gccaccgagg aagttgtcgc cagcggaggc gtgaacttca atcaccggcg
     3241 tggcgtactc aagcaccgtg acgtcaaacg tgccgccacc gagatcaaac accagcgaac
     3301 gggtattttg ttgggtatgc aggccatacg ccatcgcagc tgctgtgggt tcattaatta
     3361 agcgtaccgc atttaaaccg gctaactccg ctgctaaacg ggtatgcttg cgttgttcat
     3421 cgctgaaata agccggaaca gagatcacca catctttaat cggacgttgc agaaattctt
     3481 cggcatcttc ttttaatgag cgtaatacca aagaggacag ttctggcgcg ttaaatgtgt
     3541 cgctgcctaa ccgccagttg gtattactgc ccattgcacg tttaaataac gctgccgttt
     3601 tatccggatg cgaagtacgc cgtgatacag ccggttttcc gactaaaata tgattattt
//
LOCUS       DBFIFI010000297         3623 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.298, whole genome shotgun sequence.
ACCESSION   DBFIFI010000297 DBFIFI010000000
VERSION     DBFIFI010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.298"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            59..286
                     /locus_tag="N7B39_004610"
     CDS             59..286
                     /locus_tag="N7B39_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020232163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424441.1"
                     /translation="MACNVFAFSNSGGGSETSYKEPEKTSAMTTTHSTKFQPSQAILF
                     KMREDAPPLNLTEETTPPFPTKANYLIHPVR"
     gene            complement(363..605)
                     /gene="yfdY"
                     /locus_tag="N7B39_004611"
     CDS             complement(363..605)
                     /gene="yfdY"
                     /locus_tag="N7B39_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HGU2424442.1"
                     /translation="MFYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            958..1878
                     /gene="lpxP"
                     /locus_tag="N7B39_004612"
     CDS             958..1878
                     /gene="lpxP"
                     /locus_tag="N7B39_004612"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HGU2424443.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            2234..2305
                     /gene="ypdK"
                     /locus_tag="N7B39_004613"
     CDS             2234..2305
                     /gene="ypdK"
                     /locus_tag="N7B39_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HGU2424444.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(2370..3608)
                     /gene="alaC"
                     /locus_tag="N7B39_004614"
     CDS             complement(2370..3608)
                     /gene="alaC"
                     /locus_tag="N7B39_004614"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HGU2424445.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAILESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
BASE COUNT          927 a          925 c          851 g          920 t
ORIGIN      
        1 ataactttct atgaaagtaa acttaatact ttttagctta tttttattgg tctctattat
       61 ggcatgcaat gtttttgcat tttctaattc tggtggtggt agtgagacga gctataaaga
      121 gccagaaaaa acgtcagcga tgacgacaac tcactctaca aaatttcagc catcacaggc
      181 gattttgttt aagatgagag aagatgcacc accattaaac ctcacagaag aaaccacgcc
      241 cccttttccg acaaaggcga attatcttat tcatcctgtg cgatagcgtc tgtcacctgg
      301 caggtgaaca atcatgtctg atgctgatgt gaacacctgc ccaaatgaac catcctgacc
      361 cactatctcc tgccataccc caccataaag cgaatcatca agccacaagc cggggcaaga
      421 aacataaata tccccgtcac cagttcatta ccgccagtta accacatggt gaagtaataa
      481 atgagtgcag cgaggatcac catgccgaag aaagaagaga ccgccgatac cacgttcagt
      541 acctgaccga tgtatccgct tacgcagaca atacacaggg cgagaaacat ccataaataa
      601 aacatagctc aacctcctgc cgcgctaaaa aacctgtaac ttcagtcact ttaatagtaa
      661 gacaatcctt aacctctggc aatttttgct aagcgtcaat gaaaacagca tgttaaatgc
      721 aatactattg cgtgcggaaa attttttact ttgcacgatt aataatcagt agctgaaagc
      781 agtcagccag tgataaagta cagataccgc attcatgctc ctcgtggtta tgtcctgact
      841 agtctttaca ctctttacag gaaccattgt cgtacatgat ggcccaacca attgagtgta
      901 ccgctgtcga catggaatct tctgcgatac aacaattcgt atctacagaa ggtaactatg
      961 tttccacaat gcaaattttc ccgcgagttt ctacatcctc gctactggct cacatggttt
     1021 gggcttggtg tactctggct ttgggtacag cttccttatc ctgttctctg ctttctcggc
     1081 acgcgtattg gcgcaatggc gcgaccattc ctgaaacggc gtgaatctat cgcccgtaaa
     1141 aacctggaac tttgcttccc gcagcattct gcggaagaac gcgagaagat gattgccgaa
     1201 aactttcgtt cactcggcat ggctctggta gaaaccggca tggcgtggtt ctggcccgac
     1261 agtcgcgtac gtaaatggtt tgatgttgaa gggctggata accttaaacg cgcacaaatg
     1321 caaaatcgcg gcgtaatggt tgtcggcgtc cattttatgt cgctggaact gggcggccgc
     1381 gtgatgggac tttgtcagcc aatgatggct acctatcgtc cacataataa tcagctgatg
     1441 gaatgggtgc agacccgtgg gcgcatgcgc tctaacaaag cgatgatcgg cagaaataat
     1501 ctgcgcggca ttgtcggtgc actgaagaaa ggtgaagcgg tatggtttgc tcccgatcag
     1561 gattatggtc gtaaaggcag ctccttcgcg ccgttctttg cggtggaaaa tgtcgccaca
     1621 accaatggca cctatgttct ctcccgtctc tctggcgcag ccatgttgac cgtaacgatg
     1681 gtaagaaaag cggattacag cggatatcgt ttgttcatca ccccagagat ggaaggctac
     1741 ccgacagatg aaaatcaagc cgctgcctat atgaacaaga ttatcgagaa agagatcatg
     1801 cgcgcaccgg agcagtacct ctggatccac cgtcgcttta aaacgcgccc ggtgggagaa
     1861 tcgtcgttgt acatttaaca aactgctctt actctttccc tcctcaaatc ggccataata
     1921 acctcatggt ttttatggct tttttcttaa gattttttcc ttcgctgaat atctaattat
     1981 taccgccaga aattaaactg tcgccgatat gagacacctg taagttacta aaagtaaata
     2041 agaaaatgcc tcttgtctcg tttcagtttt aggcgtacat tagcgccgtc tcgagcagtt
     2101 tagctcagaa ttctggggtg atgcccacgg taaaatacag gacttttaag gtcgttttgc
     2161 ccggtgtcaa ctcactgtat caggtgtgat gaagtcattc aggcgtaacc gtaattacgc
     2221 ggagagatgt aaagtgaaat atttctttat gggcatttct tttatggtca tcgtttgggc
     2281 cggtactttt gccctgatga tctaaaagca aactgtgcaa tgaaaaacag gagccatcag
     2341 gctcctgttt gcatttctga cgacggtgtt tattccgcgt tttcgtgaat atgtttgctg
     2401 ctggcgggta gtaaaccgtc ggcacggaac atcgctttaa tcccacgaat cgcctgacga
     2461 atacggtcgc ggttttcaat cagtgcaaag cgaacatgcg tgtcgccgta gtcaccaaag
     2521 ccaatccctg gagagacaca gaccttcgct tcgttaagga gcttcttggc aaattccagc
     2581 gatcccatgg ccgcatatgg ttccgggatt ttcgcccaga catacatcga agccttcggc
     2641 atttcgacca tccagcccgc ttcatgcagc cctttaacca atacatcgcg gcggcgtttg
     2701 tactgttcgg caatgtcgcg cacgcactgt tgatcgccct ccagcgccgc aatcgccgcc
     2761 acctgcaacg gcgtaaaagt gccgtaatcg tgatagcttt tgattcgcgc cagggcactg
     2821 accagcgttt tgttgccgac cataaagccg atacgccagc ccgccatgtt gtagcttttc
     2881 gacagcgtaa agaactcgac tgccacatcg cgtgcacccg gtacctgcat gattgacggc
     2941 gctttccagc catcgtagac gatatcggca taggccaggt catggaccac cagcacatcg
     3001 tagcgtttcg ccagcgccac tactttttcg aagaactcca gttccacgca ttgcgcggtt
     3061 gggtttgacg ggaagccgag gatcatcatc ttcggtttcg gataactttc aagaatggcg
     3121 cgctccagtt cgttgaagaa atcgacacct tccaccagcg gcactgagcg tacctgcgcc
     3181 ccggcaatca ccgcgccata aatatggatc gggtagcttg gattcggcac cagcaccgtg
     3241 tcaccatgat ccagcgtcgc cagcatcagg tgtgccagcc cttctttcga accaatggtg
     3301 acgatggctt ctgattccgg atcgatttca acgtcgtagc gatcctgata ccagcgggaa
     3361 atggcgcgac gtaaccgcgg aatgccgcgt gaagtggagt aaccatgcgt gtccggacgc
     3421 tgggccacag tacataattt ttcgacgata tgcggcggag tcgcaccgtc cgggttaccc
     3481 atgctgaaat cgataatatc ttcgccgcgc cgacgcgcag ccattttcag ttcagcggtg
     3541 atgttaaaaa cgtagggcgg gagacgatca atgcgcgtaa agcgacgttc agggcgagtg
     3601 tcagccataa tttcctcaga tta
//
LOCUS       DBFIFI010000298         3618 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.299, whole genome shotgun sequence.
ACCESSION   DBFIFI010000298 DBFIFI010000000
VERSION     DBFIFI010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3618
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.299"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            251..925
                     /gene="tnpA"
                     /locus_tag="N7B39_004615"
     CDS             251..925
                     /gene="tnpA"
                     /locus_tag="N7B39_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HGU2424446.1"
                     /translation="MNSQTKKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFIASGIAWPLPDSVSLAQLDAILYANRKKELTEPQISEGTWRKERRTSYSRE
                     FKIRLVKQALQPGAVVARIAREHGINDNLLLKWKSQYEDGLLSDDDIQECMPVPVALT
                     DTPEPTRPVTNPFWRNKPDECPESDPGNVPRCELHLKSGVVKLFDPLTPEMLRALIRE
                     MKGGTR"
     gene            922..1269
                     /gene="tnpB"
                     /locus_tag="N7B39_004616"
     CDS             922..1269
                     /gene="tnpB"
                     /locus_tag="N7B39_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGU2424447.1"
                     /translation="MITLPTGTRIWIIAGITDMRCGFNGLASKVQNTLKDAPFSGHIF
                     VFRGRSGKMVKILWADRDGLCLFAKRLERGRFVWPVTREGKVHLTPAQLSMLLEGIAW
                     QHPKRTERPGIRI"
     gene            1289..2659
                     /locus_tag="N7B39_004617"
                     /pseudo
     CDS             1289..2659
                     /locus_tag="N7B39_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(2664..3455)
                     /locus_tag="N7B39_004618"
                     /pseudo
     CDS             complement(2664..3455)
                     /locus_tag="N7B39_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308008.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
BASE COUNT          933 a          981 c          920 g          784 t
ORIGIN      
        1 gtcacgaacg gtgcaatagt catccgtcgt tgacaacagc tgatactgag ggtttccggg
       61 tcctctgcga gcgattctgg ctgttcggca gctgcatcga gagtcacagg aagtagctgg
      121 gggctctctg aagaaggtaa tagcagcttt ccctcgcgcc attgttgtgt aagcgcccca
      181 tctgcgacgt cttgtgaaaa ttgtcctgtc tggcaacaat cgcgcccatc tatatagatg
      241 gacacgaacg atgaattccc agacaaaaaa agatattccc tgcttccgtt cttatttgcc
      301 tgatgctctg cgtttaagat ttgaagataa actgaccatc cgggccatcg ctcagcgtct
      361 aggtctcagt cattccacaa tacatacgct ttttcagcga tttattgcat ccggtatcgc
      421 atggccattg cccgattcag tttcattagc tcagcttgac gccatccttt atgccaacag
      481 aaagaaggaa ttaacagagc ctcaaatcag cgaaggcaca tggcgaaaag aacgacgaac
      541 ctcctacagt cgtgaattta aaatcaggct ggtaaaacag gcattacagc ccggtgctgt
      601 tgttgcccgg atcgccagag agcacggtat caatgataac ctgctgttaa aatggaaaag
      661 ccagtacgag gacggcttac tgagcgatga tgacatacag gaatgcatgc ctgtcccggt
      721 ggctctgact gatacgccag agccgaccag accagttaca aatcccttct ggcgtaacaa
      781 gcctgatgag tgccctgaga gtgatcccgg aaacgtccca cggtgcgagc tgcatcttaa
      841 atcaggtgtg gtaaaactgt ttgaccctct cactccggaa atgttacggg cgctaatccg
      901 cgaaatgaaa ggaggtaccc gatgataacg ctgccaaccg gtaccagaat ctggatcatc
      961 gctggcatca cagatatgcg ttgtggcttc aacggcctgg cttcgaaggt gcagaacacg
     1021 ctgaaagatg ccccgttctc cgggcatatc ttcgtcttcc ggggccgcag tggcaaaatg
     1081 gtgaaaatac tgtgggccga tcgtgacggg ttatgcctgt tcgccaaacg cctggaacgg
     1141 ggccgcttcg tctggccggt aacccgggaa gggaaagtgc acctgacgcc agctcagtta
     1201 tccatgctac tggaggggat cgcatggcaa catcccaaac ggacagaacg gcctggcatc
     1261 cggatataac ccgtgataaa acaggggaat gaacaacaca ctccccgaca acatcgagca
     1321 actgaaggcc ctgctgatcg cacagcaggc tgttatcgtc cgtctgtctg gtgaaataac
     1381 cggctatgcc cgcgagatca gctcactcag agcgctggtc gctaaactgc agagaatgtt
     1441 gttcggtcgc agcagcgaga aaagccgcga gaagatagaa aagaagatcg cacgggcaga
     1501 aacgcgtata accgagctcc agaacaggct tggtgaggcg cagttgcaac tcacctcaat
     1561 ggccggagag acagcgccga aaacatcaga ctctcccgtc cgcaaagcac ttccggcaac
     1621 acttccccgt gacaggcagg ttatctcccc ggcagaaacc gaatgccccg tctgcagcgg
     1681 taaactgaaa ccgctgggag aaagcatctc tgaacaactg gatatcatca acaccgcgtt
     1741 cagggtaatc gaaacggttc gcccaaaacg ggcctgcagc cggtgcgact gtatagttca
     1801 ggctccgcag ccaccaaaac ccatcgagcg cagttacgcc agtccggctc tgctggcccg
     1861 cataatcatg gctaagttcg ccgagcatct gccgctgtac cgtcagtcgg aaatctatgc
     1921 ccgccagggc gtggagctgc accgcaatac gatggggcgc tgggttgaca tcatgggaga
     1981 gcagcttcgc ccgctgtatg atgaactgaa gcactatgtg ctgatgccgg gtaaagtgca
     2041 tgccgatgac acgccggtaa atgtactgga gccgggtcag ggtaaaaccc gtaccggacg
     2101 gctgtgggtc tatgttcgtg acgatcgcaa cgccggttcg accatgccgg cagcggtgtg
     2161 gttctcatac tctcccgacc gcaaaggcat ccacccacag caacatctgg cggactacag
     2221 aggtatcctg caggccgatg catatgcggg ttacaatgct ctttacgaaa gcggtcaggc
     2281 aaccgaagcg gcttgtatgg cacatgcccg acgcaagatc cacgatgtac atgtccgcca
     2341 tccaacgaca gtaacgggag aagcgctccg tcgtatcggg gaactgtacg ctatcgaggc
     2401 tgagatccgc ggcagtccgg cagaagagcg actggcggtc agaaaagcca gaacggtacc
     2461 gctaatgcag tcgttgtatg agtggctcta ggggcagatg aacacgctgt cgcgccactc
     2521 ggatacagcg aaagcgttca cctatctgct gaagcaatgg gacgctctga acgaatactg
     2581 ccgcaatggc tgggtggaga tcgacaataa cctgtgtgaa aacgccctcc gggtagttgc
     2641 actggggcgg cgtaactacc ggacgtggtt tctgccaggt aaaggcaacg gaacaaaaga
     2701 atcatggttt ttccggaacc ggccacacca tgaatgacac gatgcccgtc accaatattt
     2761 ctggccagaa tctcctgctg aatatccata attttgattt gtgagttctg tttcacggtc
     2821 acttcaggaa acagatgggc gcgcagaata tccctcatcc ggggtgttac ccgggttctg
     2881 aaccccgtgg taaacatccc ggcaattttt tgccggaata cctcaggtaa aacagactgc
     2941 gtcatttcat cctgacagat tgtctgtgct gaaggaaaaa tcttttccac cgcattctca
     3001 ttattccctg tcagtgcctt cagttgctga cgggtaatgc gggtaaacac cacaccatac
     3061 gcccaggcca ggttcagccg ccccttatac tgaccgtcat tctgccgtaa gcacgggtca
     3121 gcaggtaagg cattaaccgt atcacaggca taacgacgaa cctgtaccag cggatttatt
     3181 tctgatttca gaagaccgtc ggtttccagt gtaacctgct cctgatttgc tttacgtaat
     3241 gtggatacag tccagtcctt gacctccaga aataccagcc cgttatccgg gtcaataatg
     3301 acaaaatccg gatgtcggtt tttccgtccc accgggatat cgtaccagac aaagcaatca
     3361 tcattcagaa agctctccag tcgtgaagcc accctcctct cccctggcgt cattctctcc
     3421 tcagtggacg ctgatatatt tatcagtttc gccataacat actcatgctc cagtaaaacc
     3481 ataaccgtcg acatattccg tctgaaggga atatgtcgaa tacttcatta aaatacaaac
     3541 atttagtaaa cttcactacg caagtgcaat tatccagtcc cgtctaatga ttaaaaacga
     3601 taaccaactc caacagta
//
LOCUS       DBFIFI010000299         3577 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.300, whole genome shotgun sequence.
ACCESSION   DBFIFI010000299 DBFIFI010000000
VERSION     DBFIFI010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.300"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            78..814
                     /locus_tag="N7B39_004619"
                     /pseudo
     CDS             78..814
                     /locus_tag="N7B39_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            993..1289
                     /locus_tag="N7B39_004620"
     CDS             993..1289
                     /locus_tag="N7B39_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU2424448.1"
                     /translation="MPDQSGHCRTPSRILRMPELSQLLGISRSTIYEKMNPLSKYYDA
                     TFPRPVRLGSGSVGWRSSAIDEWLTLHTVPARSAVKGVNDEVRHTLSSDMTMSR"
     gene            1597..1713
                     /locus_tag="N7B39_004621"
     CDS             1597..1713
                     /locus_tag="N7B39_004621"
                     /inference="COORDINATES: protein motif:HMM:NF024547.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424449.1"
                     /translation="MSIACQNQVDVCSPGNLRGAVNIYTMVLEKGKRPGERY"
     gene            1932..3317
                     /locus_tag="N7B39_004622"
     CDS             1932..3317
                     /locus_tag="N7B39_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HGU2424450.1"
                     /translation="MCLLAPENPYPIYALPPLVRNAIIETQKNTQAPLAMVATSALTA
                     ISIACQNQIDVCRPGNLHGPVNLYSLILADSGERKTTVDKVFMKAFYLRDEALADEYA
                     KLVENYSTEKEIWEQKQKALESKFHKEIRAGKDYKATESELETHLNKPPVPPQIRRTI
                     FNETTIEGMLKYYSDSNRSFALVSSEGGVIFDSRAMSKLGIINTLWDGGSLFIDRKSS
                     PGINLKEPRLTISVMIQPDVYHKGFCTRKKEIVKTSGHHARFLMCQPTSTQGTRIMIN
                     ELIDESLAMSGERRCLHFSPQAARIWTDYYNDVESKLGGLGPLRHCREYAAKNAEYMA
                     RLAGLIYHSSGEEGEISPYTAEMARELAIWYGNEYVRLSNPLTFDNSALTVPVRLIPE
                     ELELFNWIKSYCIEKGILCMKKNDILQRGPNRFRKKDKINWLLDLLYEQNRVVPVIEG
                     KTLCVAPNFDL"
BASE COUNT         1088 a          692 c          802 g          995 t
ORIGIN      
        1 cagattatgt cagaaggccc cggtgatgcc ttctgacgat agattaatgt tgtattctat
       61 tggtatctgg agaccatatg gcaacagtta cagtatattc tccccgctgt aattcctata
      121 aagtgtaccg ttatgatcac agcacctcag atcatgagcg tttccagtgc taatcctgca
      181 aacgtgtatt tcaactgaat tacatctacg aaacccgaaa accaggcatt aaagagcaaa
      241 ttgttgaaat ggttcataac ggagccgggg ttcgcgatag ctccagaacg ctgaaggtgg
      301 atatcaacac cgtcattctc actttaaaaa acgctcacca cgtaaagtaa ccatatctcc
      361 agccgccagt gtcgatgtgg cacttatctg tgaacttgat gaacaatgga gttttgtcga
      421 aaacaaagct cgtcagcagt ggcactggta cgcgtataag accaaagctg acggtgtgct
      481 ggcttacact tttggtcctc gcactgatga aacatgccgt gagttgccgg aattcctgaa
      541 gccattctcc gcaggcatga taaccagaga caaccggagc agttatacca gagaaatgcc
      601 acaggataag catcttgtcg gcaagatatt tacccggcgc attgagcgca acaatctgac
      661 acttcgtact cacatcaaac gacctgcccg gaaaacgatt tgctttttac gctccctgga
      721 aattcacgaa aagccattgg ttcatttatc gaaaacacat gtactactga caggagtcat
      781 taccagggcc tcttttgctg tttttttgcc atgaatgata atgagaatcg acagcgataa
      841 gggaaaagta aaataacact aatgctgtcg ttttctatca tattgatttt taattattta
      901 ttgtcttttt tctcaaccca tttttttacg cagacaaatt tacccgggga atacaaactg
      961 taacgacagt tcaccacact atgggggata aaatgccaga tcaatccgga cactgcagga
     1021 ctccttcacg catcttacgg atgcctgaac tgagccaatt gttaggtata tctcgttcga
     1081 caatttacga aaaaatgaat ccgttatcga aatactacga tgcaaccttc cccaggccgg
     1141 tacgtcttgg tagtggttct gtgggctggc gttcatcagc tattgacgaa tggcttactt
     1201 tacatactgt acctgccaga tcagcagtca aaggtgttaa tgacgaagtg agacatacgc
     1261 tttcttcaga tatgaccatg agcagataag tttttgtcat cacgtgatca cctcaggagg
     1321 aaaaagaagc ataagaacaa tttagccaga agtttaattg ataaacagaa tttccagcat
     1381 tcattgttga aagagcagct attgtcgcta ttgtagcggt aattgctgct ataaccgaaa
     1441 cgtattacga aaaaggaagc atttatgcct tacatcaaac cggatatgtc gtattcagta
     1501 tatgcatgac catcaatgat agggaaggca ataattaaag ctcaatacaa taaacaagta
     1561 cctctgactc tggttgcaat gtcagtatta acagcgatgt caattgcctg tcagaatcag
     1621 gttgacgtat gcagtcccgg aaatttacgt ggggccgtta atatttacac tatggttctg
     1681 gagaaaggga aaagaccggg ggaaaggtat tagtgaaata atttccggag gtattaagaa
     1741 tgagaaatat gaatgatatt tcgtggtgaa taatacagtc ttcactgaat taaccttatc
     1801 ggtttattaa acatcagatc tacaaatgct ctgtatttct cagagcatca ggtctgaata
     1861 gcagctattt tcgctattgt agcgggattt gctgctattt tactaaccca ttaatgaaaa
     1921 gagaggcttt tatgtgttta cttgcaccag aaaatcctta tccgatatat gcactaccac
     1981 cgctggtgag aaatgcaata attgaaactc aaaaaaatac acaggcacct ttggctatgg
     2041 tggcaacatc cgcattaaca gcgatctcaa ttgcctgtca gaatcagatt gacgtgtgca
     2101 gacctggaaa tttacatggg cctgttaatc tttactctct gattctggct gattccggtg
     2161 aaaggaaaac gaccgtggat aaagtgttta tgaaagcatt ttatctcagg gatgaagccc
     2221 tggcggatga atacgcgaaa ctggttgaga attacagtac agaaaaggaa atatgggagc
     2281 aaaaacaaaa agcgctggaa tcaaaatttc ataaagagat tcgtgccggt aaagattaca
     2341 aggcaacaga atcagagctt gaaacgcatc tgaataaacc tcctgttccg ccgcagatac
     2401 gtcgaacaat ttttaatgag acaacgatag agggaatgtt aaaatattac tccgatagca
     2461 atcgctcttt tgctcttgta tccagtgaag ggggggtaat ttttgacagc cgggccatga
     2521 gtaaactggg aattattaac actttgtggg atggaggttc tcttttcatc gacaggaaat
     2581 catctcccgg aattaatttg aaggagccaa gactgacgat atcggtgatg attcagcctg
     2641 atgtttacca caaaggtttt tgtacgcgaa aaaaagaaat tgtgaaaacg tcaggacatc
     2701 atgcaaggtt tttgatgtgt caaccaacat caacgcaggg gacaaggata atgatcaatg
     2761 agcttattga tgaaagtctg gcgatgagtg gtgaacgacg ttgccttcac ttttctcctc
     2821 aggctgccag aatctggacg gattattaca atgatgtgga atcgaagctg gggggattgg
     2881 ggcctttaag acattgccgg gaatatgctg ctaaaaatgc agagtatatg gcaagactgg
     2941 caggacttat ttaccattcg agcggtgaag agggggagat ttccccttac actgcagaaa
     3001 tggcgagaga attagcaata tggtacggta atgagtatgt gcggttgtct aatccgttaa
     3061 cttttgacaa ctctgccctg accgtacctg tgcgactcat tccagaggag cttgaacttt
     3121 tcaactggat aaaaagctat tgtattgaga aggggatcct ctgtatgaaa aaaaatgaca
     3181 ttttacagcg tggtccgaac cgtttccgga aaaaggataa aatcaactgg ttactggatt
     3241 tactgtatga acaaaacaga gttgtaccgg ttattgaggg aaaaacgttg tgtgtcgcac
     3301 ctaactttga cctctgaacg acttaatcgt tgagtacata tagtctcctc tgctacttgc
     3361 ggaggagact aatgtcttta ccatgtcagc gttgtattag aaaactctgg tacaggtcag
     3421 tcagtaaata taactggtgt aaccactatt tactattgga tagagtgttg ctcaggatat
     3481 tgtcggcttt agtctgaggt gaggtggggc cttcattgtt ttcgtgttca ataaataatt
     3541 taggttaatt agtattatta gtggaagtta tgtgatc
//
LOCUS       DBFIFI010000300         3574 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.301, whole genome shotgun sequence.
ACCESSION   DBFIFI010000300 DBFIFI010000000
VERSION     DBFIFI010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3574
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.301"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(178..543)
                     /locus_tag="N7B39_004623"
     CDS             complement(178..543)
                     /locus_tag="N7B39_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HGU2424451.1"
                     /translation="MAVLRTLPGRIKTLNTRRVNILKGEQRRVSGSARVSLKRHIWLR
                     DAGQCCLCGRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSAREAAGGMPD
                     PTLPEVSGGHGRADDIIGL"
     gene            585..812
                     /locus_tag="N7B39_004624"
     CDS             585..812
                     /locus_tag="N7B39_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HGU2424452.1"
                     /translation="MSSKNRPRRTTTRNIRFPNQMIEQINIALDQKGSENFSAWVIES
                     CRRELAADIKYARQLTIKKNDTQYALRWLFI"
     gene            complement(1275..1532)
                     /locus_tag="N7B39_004625"
     CDS             complement(1275..1532)
                     /locus_tag="N7B39_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424453.1"
                     /translation="MSDRVIECASRAGRDFSEFMKGEKGMMEALASVDEFGEQLRLNG
                     CVNHHFVSYMMRNSIMQAFMDMAKAERKEERRRKRAEAKAK"
     gene            complement(1529..2026)
                     /locus_tag="N7B39_004626"
     CDS             complement(1529..2026)
                     /locus_tag="N7B39_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HGU2424454.1"
                     /translation="MKSLTLFNQPIRIGEDGMICLTDMWKASGKSESESPYHYLRNKQ
                     TKEFLAELEKNHESVVFTERGAHGGTYGGKFVAYDYAAWLNPGFKYAAYKVLDDYFTG
                     ELHHRNSLSAQLNMKCHEFDQKKDMASFCGQGLAAWRYTKPGLIAEINSLANQLQISI
                     PGLPG"
     gene            complement(2229..2666)
                     /locus_tag="N7B39_004627"
     CDS             complement(2229..2666)
                     /locus_tag="N7B39_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HGU2424455.1"
                     /translation="MSRVTVIISALVICIIACLSWAVNHYRDNAIAYKEQRDKATSII
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVSATCAKSTTGASSM
                     GDGESPGLTADAELNYYRLRGGIDKITAQVNYLQEYIRTQCLK"
     gene            complement(2663..3160)
                     /gene="rrrD"
                     /locus_tag="N7B39_004628"
     CDS             complement(2663..3160)
                     /gene="rrrD"
                     /locus_tag="N7B39_004628"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HGU2424456.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMPGKTYTEAECKALLNKDLATVARQINPYINVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            complement(3160..3375)
                     /locus_tag="N7B39_004629"
     CDS             complement(3160..3375)
                     /locus_tag="N7B39_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HGU2424457.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            complement(3452..>3574)
                     /locus_tag="N7B39_004630"
     CDS             complement(3452..>3574)
                     /locus_tag="N7B39_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424458.1"
                     /translation="GVQAEPPTEAGDTTAESGEVQPESPVADATEPQPESVMML"
BASE COUNT          877 a          961 c          820 g          916 t
ORIGIN      
        1 gttaacattt tttccgcaca acaaactgaa taataaagat aaaaaccgca aaaatgcccg
       61 gacagccagt taacatgtta actgccctga aacgggaatt ttttctctgc gtgagacggg
      121 gggcggtgtc cggagcgatc gtttttttcg ccggatgatc cccccccccg gggcgggtca
      181 cagtccgatg atatcgtctg ccctgccatg acctccggac acctccggca gcgtcgggtc
      241 cggcatacca cccgccgctt cacgagcaga cttttgtcga tggcattcgg tacagagcgt
      301 ccagagattc gtctcctcat taccaccacc gaactgaagt gcaattcggt gatcgagttc
      361 actgtcacag aggtcaacca cacgaccaca gagacagcac tgcccggcat ccctgagcca
      421 gatatgacgc ttgagggaaa cacgtgcact gccactgaca cgacgctgtt cacccttcag
      481 aatattcacc cgccgggtgt tcagagtttt gattctgccc ggtaacgtac gaagcacagc
      541 catgtaaaat cctcgccata tagcttgtca ccagaggaaa gaaaatgtca tcgaaaaacc
      601 ggccccgcag aacaacaacc cgcaacatcc gatttccaaa ccagatgatt gaacaaatta
      661 acatcgctct tgaccagaaa ggttcagaaa atttttctgc gtgggtcatt gaatcttgcc
      721 gccgggagct ggcagcagac ataaaatatg cccgtcagtt gactataaaa aagaatgata
      781 cacagtatgc tctgcgatgg ctgttcatat aactatttct ttatattgct gaatttataa
      841 aaactcacag acattagctg tattaattcc gaattgaaat aatcagccat atagaataaa
      901 aataaagcat aacaataata atcttctacc caatcagtac attactgctg tgactccaac
      961 acggcagttt ttttattgaa cagattccag tttcttccac catcgcaccg gacgggcgac
     1021 catgagggga gaacgccgcg ctccgtttac gcggtaaacc ccggtgtgta tcgtttttga
     1081 ttatccccgc acactcgcgc agaaggaatt ccccgtcggg ctacggtcat ggttaatgcg
     1141 gaaatacagc gacgatacag cgcatgatgt gtcaggcttg aataccttta tccgttaaaa
     1201 gggatatcag ttaagttatc ccgtgtaggg tataagccat tatcaagccc accagtagat
     1261 gggctttgta atggctactt cgcttttgct tccgctcgct tacgccggcg ctcttctttc
     1321 ctctcggctt ttgccatgtc catgaatgcc tgcatgatcg agttccgcat catgtagcta
     1381 acaaagtgat gattgacaca gccgttgagg cgcagctgct cgccaaactc atccaccgag
     1441 gccaatgctt ccatcatacc cttctcgcct ttcatgaact ctgagaagtc gcgccccgct
     1501 ctggaggcgc attcaatgac acgatcactc atcccggaag cccggggatc gaaatctgca
     1561 actggttagc cagggagtta atttcagcga tcaaaccagg tttcgtgtag cgccatgccg
     1621 cgaggccttg tccacagaag ctcgccatgt cttttttctg atcaaactca tgacatttca
     1681 tgttgagctg cgcacttaag ctgttccgat gatgaagctc tccggtgaag taatcatcga
     1741 ggactttata ggctgcatat ttaaatccgg ggtttagcca tgctgcgtaa tcataagcaa
     1801 cgaactttcc gccataagtt ccaccgtgcg caccgcgttc cgtaaaaacc acagattcgt
     1861 ggtttttctc cagctcagcc aagaactctt tggtctgctt gtttcgcagg tagtggtacg
     1921 gagattcaga ttcactttta ccactggctt tccacatatc agtgaggcag atcatgccat
     1981 cttcaccgat acggattggt tgattgaaga gggttaatga tttcatagcg tgtacctact
     2041 ctttgaaatg aacctttgcc gcacaggaaa ccagcccacc gaggctcgcc agcactaact
     2101 ggtatcctca aaggcccatt ccaaaggggc aggttcggtg taaaaaacat gcgttgcggt
     2161 acgcatttat tgcaaaaagc cccgcatcgc gaggctcatt aaatggactt tgtgatttgc
     2221 aaaaaaaatt atttcaggca ttgcgtcctg atgtactcct gcaggtagtt aacctgcgcg
     2281 gttatcttgt cgattccacc tcggagacgg taataattga gttcagcatc tgctgtaagt
     2341 cctgggcttt ctccatcgcc catgctgctg gctccggtcg ttgactttgc acaggtggcg
     2401 gagacttgca ggcgcttacg cccagcagaa acatcagcac ggagactttc gatagtcgca
     2461 ttagcatcag caagctcctt tgtgtatctg gcgtcaagtt ctgctacatc acgttgccgc
     2521 ttctgcatat cagcgatgat ggatgtggct ttatcgcgct gctctttgta ggcgatggcg
     2581 ttatcacggt aatgattaac agcccatgac aggcaggcga taatgcagat aaccagagcg
     2641 gagataataa cggttaccct gctcattgtt gcccccacaa acagacttca cgctcaatct
     2701 cgcggcgagt catcagccct ttccattgct taccgccagc gtatgtccag cgccgtagct
     2761 gatcacatgc gcctttgata tcgccctggt ttattttgcg aagaagcgtc gatgttctga
     2821 aattgccagc gcccacgttg taaacgaacg agtaaagagc gccgcgcgtt gtttccggta
     2881 tatcgacgtt gatgtacggg ttaatttgtc tggcgaccgt ggcaaggtct ttattcagga
     2941 gggctttgca ttctgcttcg gtatacgttt taccgggcat gatgtctttt ccggtgtgtc
     3001 cgtgacatac agtccatacg ccaacgatat ctttgtatgg tatgtagctg acaccttcca
     3061 gaccatcgtt accacctgga ccagtgatga gcacagacgc tatggcaaca gccccaccac
     3121 caatagcagc tgcaacagcc ttgcgtaatg acggcgacat tattcacctc tcgcagcctt
     3181 acgcttatct tctttaatct tgaaataaag atttgtcaga tacgtcagca ggccaaacag
     3241 cagacttccc agcacaccta ttgccaccca ctgggacgga gagactttgt ccagcagctg
     3301 cagtaaccag tatcccgtcc ccaccgctga cgtggtgtat gacacacctg ttgtgatttt
     3361 ttccatctgg tacatacccc gtctcccgtt atccggaagc tcacaacatg aaaaaggcca
     3421 gcagctgttt actgatggcc ctgactcccc gttacagcat catgaccgat tcgggttgag
     3481 gttcagtcgc atcggcgacc ggtgattcag gctgaacttc accgctctct gcggtggtat
     3541 ctcccgcttc agtcggtggc tctgcctgta cacc
//
LOCUS       DBFIFI010000301         3528 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.302, whole genome shotgun sequence.
ACCESSION   DBFIFI010000301 DBFIFI010000000
VERSION     DBFIFI010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.302"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..890)
                     /locus_tag="N7B39_004631"
     CDS             complement(<1..890)
                     /locus_tag="N7B39_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HGU2424459.1"
                     /translation="MATWQQGINSGGFLAGIGGQNSNAPKASDVSEALAYIRQNNEME
                     RSGRNNIGLQALQGLGSVAQTSQAAKQQEADAAFQKEYAAAIQSGDRQQVRDLMTKYP
                     GQLEKIQSGMKWADEDQRNSIGTLAAGARLAASSPEAMQSWLQNNAKELARVGVDPNN
                     VAQMYQQNPSGFGEFVYHLGMAALGPIDYFNVQDKMAGREIDRGRLAETIRSNQAGDF
                     EFKSKPSYLVLSFAEISSVVFILMSVYMVAKSQNSNGLQYDALFIPSMAFSILVFSFN
                     GGIISKIISNKVMILLGDAS"
     gene            complement(900..1580)
                     /locus_tag="N7B39_004632"
     CDS             complement(900..1580)
                     /locus_tag="N7B39_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HGU2424460.1"
                     /translation="MLVLSESFKNKLLPMNGYMKGGSDSGSKAQARATEKGIELQREM
                     WQTNMQNLAPFTPLAQQYVSQLQNLSSLQGQGQALNQYYNSQQYKDLAGQARYQSLAA
                     AEATGGLGSTATGNQLAAIAPTLGQNWLSGQMNNYNNLANIGLGALTGQANAGQNYAN
                     NVSQLYQQQAAASAANANKPSGLQSFATGAIGGAASGAMIGSAVPVIGTGIGALAGGV
                     IGGLGSLF"
     gene            complement(1567..2034)
                     /locus_tag="N7B39_004633"
     CDS             complement(1567..2034)
                     /locus_tag="N7B39_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HGU2424461.1"
                     /translation="MEIKLIDNPVKLAEFLNNPVNTGNIVDSGDKYYIKPDAVYLGIY
                     EGLVLAGVHEVRNFWHSVVECHAVYDPGFRGEYALQGHRLFCKWLLENSPFLNSITMV
                     PDTTKYGRAIIRLLGATRVGHLDDAYMSNGKPVGITLYQLPRSKYEELLNVST"
     gene            complement(2034..2570)
                     /locus_tag="N7B39_004634"
                     /pseudo
     CDS             complement(2034..2570)
                     /locus_tag="N7B39_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail needle knob protein"
     gene            2634..3281
                     /locus_tag="N7B39_004635"
     CDS             2634..3281
                     /locus_tag="N7B39_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HGU2424462.1"
                     /translation="MVNERSINGQAGKIVSKWVTLANNAIPSNVPPQWDLDLKGQQYS
                     EPTKCIGKQIDYMAKPETKLNDFNREASKSLYDWSDANVWGYSNGWARHEIKKETLSI
                     TGFHAVRRILKAVQASRKDNYKAKRQIQRTLKRQDINRSPITPFQAPLISKHDWDLSV
                     IAVTKEAETERRELLSSQIINSNKVQTHPLLRMLRRARVRASRAKRESGGYDHYY"
BASE COUNT          973 a          848 c          823 g          884 t
ORIGIN      
        1 aaagaagcat cacctaaaag tatcataact ttatttgata ttatttttga aataatacca
       61 ccattaaacg aaaacactaa tattgaaaag gccattgatg gtataaaaag cgcatcatac
      121 tgtaatccat tgctattttg actttttgcc accatataga cagacattag gataaagact
      181 acagatgata tttcagcaaa tgatagaact aaatatgacg gctttgactt aaactcaaaa
      241 tctccggcct gattgctgcg gattgtctct gccagcctgc ctcggtcaat ctcacgacca
      301 gccatcttgt cctgaacatt gaagtagtca atcggaccga gagcagccat cccaaggtga
      361 taaacaaact caccaaaccc tgaaggattc tgctgataca tctgagcaac gttgttaggg
      421 tcaacaccga cgcgcgccag ttccttggcg ttgttttgca gccatgattg cattgcttct
      481 ggagacgagg ccgcaaggcg tgcgccagcc gctaaggtgc cgatagaatt gcgctggtct
      541 tcgtctgccc acttcatacc agactgaatc ttctctaatt gaccaggata tttggtcatc
      601 agatctcgaa cctgctgtcg atcgccggac tggatggctg ccgcatattc tttttggaat
      661 gcagcatccg cttcctgttg ctttgcggct tgagatgttt gagcgacact accaagtccc
      721 tgcaacgcct gaaggccgat gttattgcga cctgaacgct ccatttcgtt gttctggcga
      781 atataggcca acgcctcact tacatcactt gcctttggcg catttgagtt ttgcccaccg
      841 ataccagcaa gaaaaccgcc tgagttgatt ccttgttgcc aagtagccat attcccacct
      901 taaaacaatg atccaagacc accgataaca ccgccagcaa gagcaccaat accagtccca
      961 ataacaggaa ctgcactacc aatcattgca cctgatgcgg ccccaccaat ggcacctgta
     1021 gcaaaactct gtaggcctga aggcttattc gcatttgctg ccgatgctgc cgcctgctgt
     1081 tgatacaatt ggctgacatt gttagcgtag ttctgtccgg cgtttgcctg acctgtaaga
     1141 gcaccaaggc cgatatttgc cagattgttg tagttgttca tctgacctga cagccagttt
     1201 tgaccgagtg taggtgcgat tgctgctaac tggtttcctg ttgctgtaga gcctaatcca
     1261 cccgttgcct ctgctgctgc cagactctga tagcgcgcct gccctgcaag gtctttatac
     1321 tgctgagagt tgtaatactg gttaagcgcc tgaccttgcc cctgaagaga ggaaagattc
     1381 tgcaactgtg atacgtactg ctgagcgagt ggcgtgaacg gtgcaaggtt ttgcatgttc
     1441 gtctgccaca tttcacgctg cagttcgatg cccttttcag ttgcgcgtgc ctgggcttta
     1501 gatccggagt cgctgccgcc tttcatatac ccattcatgg gaagcaattt attcttgaag
     1561 ctttcgctaa gtactaacat ttaatagctc ctcatatttc gaacgaggta attgatagag
     1621 ggtgattcct accggttttc cgttactcat gtacgcatca tcaaggtgac caacacgggt
     1681 agcgccaagc aaacggataa ttgcccgtcc gtatttggtg gtgtcaggaa ccatggtgat
     1741 gctgttaagg aatggtgagt tttcgagaag ccatttgcag aataatcgat gcccttgtag
     1801 tgcatattcg ccacggaatc cggggtcata caccgcatgg cattcaacaa cgctatgcca
     1861 gaagttacgc acttcatgaa ctccagccag cactaatcct tcgtagatgc cgagatatac
     1921 cgcatcaggc ttgatgtagt atttatctcc actgtctacg atattccccg tgtttaccgg
     1981 gttgttaagg aattctgcaa gtttcaccgg gttatcgatg agctttattt ccatcactgc
     2041 tccgcaatga ttttgatggt tgtggcagta aacgccgcgc cattcgactg aatggttaac
     2101 gtgctgccat ttgtggcaag aaagccgtct ttatccacgc tgaagaacgt agctaacagg
     2161 atgttgtcgg ttgttgttgc cgagttgcga ctgcttacta gcgtgtcagg aacagaaccg
     2221 gagaatgtta gctgcattga cctgttggcg gttccgctgg gccacgtccc gacaatcgac
     2281 agcttgaaga acaaggtttt gttctcgttg aacacaacca tcttgttgtt aacggtgtcg
     2341 aagaatggtg ccaacgtccc ggatgacggc gtaagcgttt tcagcaggct aacaaggttg
     2401 gtcggcgctg tcgggatggt tactgatacg ccagagtaaa caacctctga cttcttgcgc
     2461 gtagtggcat actccagagc attaatgcgc gtttcatggt ctgaaacctg cgattccagc
     2521 gactgaactc tggtatcaag cgatgcaata tcgctttcat tcaaggcaat cagtgggttt
     2581 acgtcatgga gtgggctcaa caccagtcag agccgcatgt acacgcaata atcatggtga
     2641 acgaaagaag cattaatggg caagctggca aaattgtcag caaatgggta acactggcaa
     2701 acaatgcaat cccatcaaat gttcctcccc agtgggatct agatttgaaa ggtcagcaat
     2761 attcagagcc tacaaagtgt ataggtaaac agattgatta catggcaaag cccgaaacta
     2821 agttaaatga ctttaataga gaggcgagca agagcctata tgattggtca gatgccaacg
     2881 tttggggtta ttcgaatggc tgggcgcggc atgagataaa aaaagaaaca ctttctatca
     2941 ctggctttca tgctgtaaga cgcatactaa aagccgttca ggcatcaagg aaagacaact
     3001 acaaagctaa gcgacagata cagcgtacat tgaaacgaca agacataaat cgatcaccaa
     3061 tcaccccgtt ccaagcaccg ttaattagca aacacgattg ggatttatca gtgatagcgg
     3121 tcaccaaaga ggcagagaca gagcgcaggg aattactatc ctcacagata ataaactcga
     3181 acaaagtaca aacccaccca ctgttaagga tgttaagaag agcacgagtg agagcatcca
     3241 gagccaaaag agaaagcgga ggttatgacc attactacta gtagtgacag atcgtgacaa
     3301 ggcggtgcaa aaaacgacca ataataagag agatatgcct gccaaaccgc caaaagcgcg
     3361 gagcgcggca aacgccgcga gaggcgcaag aaggcgaacc tatccattga aacggtcaga
     3421 agcgcatcgc gctcgacgac cgaagtcccc actaacaccg ttaacgaaag atcaaagtct
     3481 tagaaaaaaa tgacgtttct ttgatggact tccatccgta aaaatcaa
//
LOCUS       DBFIFI010000302         3504 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.303, whole genome shotgun sequence.
ACCESSION   DBFIFI010000302 DBFIFI010000000
VERSION     DBFIFI010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3504
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.303"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..1127)
                     /locus_tag="N7B39_004636"
     CDS             complement(<1..1127)
                     /locus_tag="N7B39_004636"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HGU2424463.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSAIPFLWQHDHSRPVGQCTVRRVSEGLEITATLVKPVPDMPSQLAARLDEVWAAIK
                     TGLVRGLSVGFRPHEYTFLDGGGLHFLRWELMEVSAVTVPANAECTIRTIKSYDRPFS
                     AASGNRKPVVKIASSAGAAAQSTTVFHKEKTIMNIGEQIKSFENKRAALAASLEEVMT
                     KAAEEGRTLDVEEEEHYDNTAAEIRQVDAHLKRLRELEAGKAATAQPVKQAGNGNVAA
                     VASAPVIRVEQKLDKGIGFARFAKSLAAAKGVRSEALEVARRQYPDDSRLHHVLKSAV
                     GAGTTTDPQWAGSLSEYQEYAQDFIDYLRPQTIIGRFGQGG"
     gene            complement(1191..2852)
                     /locus_tag="N7B39_004637"
     CDS             complement(1191..2852)
                     /locus_tag="N7B39_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HGU2424464.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPAPIYPEQAAHALAIFKQLRI
                     VDAPGSPTFGESCAQWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGGFRQKLQYARDVRDGKIHDPHFLPVIFEHPPEMVESGAHLLMENLAM
                     VNPNLGYSVDEAFLYREYRKAREAGEEAFRGFMSKHANVEIGLALRSDRWAGADFWEQ
                     QGRRVSLDDILQRADVVTVGIDGGGLDDLLGMYVIGRDRETREWLGWGHAWAHETAVV
                     RRKSEASRFQDLVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLDHIGIDPSGVGQIL
                     DSLAEAGIPDGIVVGISQGWKLGGAIKTTERKLAEGVLVHGDQPLMAWCVGNARVEPK
                     GNAILITKQASGRGKIDPLMALFNAVSLMSLNPEPKKKEYAVFFI"
     gene            complement(2849..3412)
                     /locus_tag="N7B39_004638"
     CDS             complement(2849..3412)
                     /locus_tag="N7B39_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HGU2424465.1"
                     /translation="MLTTQKRKFALALMSGKNKTASALAAGYSAKTARVKGSQLAKDP
                     EVLAFIARKQCETVEVDEVPVYRQKKSEPEDKPRRREAAAIPQPDETNPEMPPPVVIS
                     PGIEYMEDGLPDPVKAMGRLLVENINTDPRLALDAAYKLAQFTHHKKGDAGKKSAKGD
                     AAKKAANRFAVPPPPRLVVNNDNEGNG"
BASE COUNT          707 a         1137 c          888 g          772 t
ORIGIN      
        1 atcccgccct gaccaaatcg cccgataatg gtctgcggac gcaggtaatc aataaagtcc
       61 tgtgcgtatt cctgatattc agacaggctg cctgcccact gcggatccgt ggtggtcccc
      121 gcgcccactg ccgatttcag gacatgatgc agacgactgt catccggata ctgacgacgg
      181 gccacttcca gggcttcaga tcggacgcct ttagccgcag ccagcgattt ggcaaagcgg
      241 gcgaagccaa tccccttatc cagtttctgc tccacacgga tcaccggcgc agaagccacc
      301 gcggccacat tcccgttacc ggcctgtttc accggctgcg ccgtggcggc cttaccggct
      361 tccagttcac gcaggcgctt caggtgcgca tccacctgac ggatttccgc tgcggtgttg
      421 tcgtaatgct cttcctcctc cacatccagc gtgcgccctt cctctgcggc tttggtcatg
      481 acctcctcaa gggaggctgc cagcgctgca cgcttgtttt caaaactttt aatctgttcg
      541 ccaatattca ttatggtctt ttccttatga aaaacggttg ttgactgtgc cgcagcgccg
      601 gcagaagatg cgattttcac caccggtttc cggttgccgg acgcggcaga aaacgggcgg
      661 tcgtaagatt taatggtccg gatggtgcat tccgcattcg cgggcacggt gacggcagac
      721 acctccatca gttcccagcg cagaaaatgc agtccgcctc cgtccagaaa ggtgtattca
      781 tggggacgga agcccacgga cagccccctg accagcccgg tcttaatggc cgcccagacc
      841 tcatccagcc gggcagccag ttgcgacggc atatccggta cgggcttcac cagtgttgcc
      901 gtgatttcca gcccttcgct gacccggcgc accgtacact gccccaccgg gcgggaatgg
      961 tcatgctgcc agagaaacgg gatcgcactg ccaaactccg cgccctccgg ctccaggatg
     1021 tcaccatccc gatccggaga aggcgttgac gcaatcccgg tgatcacccg ttcatcctca
     1081 ctgaaggatt tcaccgtcag cagggaacag gcccgtttaa gagtcacatc agcctcctga
     1141 aaataaaaaa accgccgcag cggttcatga tggttacagt gtgaacaggg ttatatgaaa
     1201 aaaaccgcat attctttctt tttcggttcc gggttaaggg acatcaggga gaccgcattg
     1261 aacagcgcca tcagcgggtc aatttttccc cgtccactgg cctgtttggt gataagaatg
     1321 gcgttacctt taggctccac ccgggcattg ccgacacacc aggccatcag gggctggtca
     1381 ccatgcacca gcaccccttc agccagtttg cgctcggtgg ttttaatggc cccgcccagt
     1441 ttccagccct ggcttatccc caccacaatt ccgtcgggga tcccggcttc cgccagtgaa
     1501 tccagaatct gccccacccc tgacgggtca ataccgatat ggtccagtaa ctcagcctca
     1561 tgaatgcgac gcacatattc cgccacttcc gccgtgtcat ccccgacacg ccggacaatg
     1621 gtcatatctc cacaggcaac aagatcctga aaccgggacg cctcgctctt ccgtcggacc
     1681 accgcggttt catgcgccca ggcatggccc cagcccagcc attcgcgggt ttccctgtca
     1741 cggccaatca cgtacattcc cagcagatca tccaggcccc cgccgtcaat ccccaccgtc
     1801 accacatcag cgcgctgcag gatatcgtcc aggctgacgc gcctgccctg ctgctcccag
     1861 aaatccgcac ccgcccagcg gtcagaacgc agggcaagac caatttccac attggcatgt
     1921 tttgacatga agccacgaaa tgcttcctca ccagcctccc gggctttacg gtactcccgg
     1981 tacagaaaag cctcatccac cgaataaccg agattcgggt taaccatggc gaggttttcc
     2041 atcagcaggt gagccccgct ttccaccatt tcaggaggat gctcaaaaat caccggcaga
     2101 aagtgcggat catgaatttt gccgtcacgg acatcccggg cgtactgcag tttctgtctg
     2161 aaccccccgg ctggcggctc attcgactgg gtggtcgtat acaccacaaa cccttccggg
     2221 cgggaggcaa ggccgcctat ggcttcacgt aacatgtcct ccgctttgta ctgcttgcca
     2281 aataaccaca gttcatcaat cagcgtcccc acggacttga taccggatac cgtattcgga
     2341 tcggcagcca ccaccttcag ggtggtgtcc gtcaccctgt gggtgatggt ccggatatgt
     2401 gtctgcacct gacagaggtc atccagatca tcgtcccgtc gtaccatatc cctggcaggg
     2461 ttgaaggcgt tagccgccac ctccacggtc ggggccagaa tggtgtagcc cgccgcctgc
     2521 cgccagttca gtaacagcgc cgtcatcatg atcccggcag ccagcgtgga cttgctgttt
     2581 ttcttgggga taaggataaa aacttccttg atatggcgta caccggtctg cgcatcgtag
     2641 gagccaaaca gggccgccac caggtcaaac acccactgtg cgcaggactc cccgaacgtc
     2701 gggctgcccg gtgcatccac aatccgcagt tgtttaaaaa tcgccagggc atgtgcggcc
     2761 tgctccggat aaatcggagc cggaataatc gacagcccct ttttcaggcg ctctgcccag
     2821 tccgggcagg ccgtgctcca cacaggtatc atccgttgcc ctcattatca ttattcacca
     2881 ccaggcgggg tggtggtggc accgcaaaac ggttagccgc ttttttcgcc gcgtcacctt
     2941 ttgccgattt tttaccggca tccccttttt tgtggtgcgt gaactgcgcc agcttataag
     3001 ccgcatccag cgccagcctg gggtcggtat taatgttctc caccagaaga cgccccatcg
     3061 ctttcaccgg atcgggaaga ccgtcctcca tatactcaat accaggagat atcaccacgg
     3121 gcggtggcat ctccggattt gtttcgtccg gctgtggtat tgcagccgcc tcacggcgac
     3181 ggggtttatc ctccggctct gattttttct gccgataaac aggaacctca tccacctcca
     3241 ccgtctcgca ctgtttacgg gctataaacg caagcacctc cggatctttt gccagctgcg
     3301 agcctttaac cctggctgtc ttcgccgaat aaccggcggc aagggctgac gctgttttgt
     3361 ttttcccgga catgagcgcc agcgcaaatt ttcgtttttg cgttgtcagc acagcctcct
     3421 cccgggtcca gaacgcactc agccgggtat ggttcagccc atttttcccg gcgtctcatg
     3481 ccgcaaatgt taactgctgc ctgg
//
LOCUS       DBFIFI010000303         3495 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.304, whole genome shotgun sequence.
ACCESSION   DBFIFI010000303 DBFIFI010000000
VERSION     DBFIFI010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3495
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.304"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            314..1822
                     /gene="putP"
                     /locus_tag="N7B39_004639"
     CDS             314..1822
                     /gene="putP"
                     /locus_tag="N7B39_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HGU2424466.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPSVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEG"
     gene            complement(1971..3314)
                     /locus_tag="N7B39_004640"
     CDS             complement(1971..3314)
                     /locus_tag="N7B39_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424467.1"
                     /translation="MRPISNLNTSAATYIPPQQLPSSCLETLTLLVQMNNYIKRDADY
                     STGMAVAPLVPEEVHLLAAAMTTELHRHQFQPVVSANDLQLPEPFTFDVAGFTITFTK
                     TQEHDNTGNLMKIAVSKSGVCTSTNITLELFHSIVTTLMSRSQYGTFDLWSVRPILTD
                     ESQNRVHEAARYSPAQQYGREETHFTNNGMREFGNMSPLAWRNDVELQQSCNTSNIPP
                     NAANDDINNTRQTIEDQPEADEPQQYVKLTVDDMRKWAAMDQQARNALQGVSGWCTRN
                     HFNIKNARNYLTDHGLNYAGQVKVNRPHEYAKFTLEHIRQWAALNKHVRKPVGYLEKW
                     CKERNLAPTTARNYLKNDGLTALGELKLTGPQKWVTFTFGDIVQWANMTQEERNSAGG
                     AEKWSKKRGFQWSTARSYLKSSGVTKQGARKLAWLKNSGNMSNPFYLAQPTSRRT"
BASE COUNT          732 a          802 c          962 g          999 t
ORIGIN      
        1 aaatgaatga aatgttaata aaagaaatcg atatgacagg gattaaaaaa ataactcaga
       61 ctttttctct gcggcagtta acatttttga aaggtgcaac cgcaaaaaat gtgagagagt
      121 gcaacctgat gaaaaatagt gtcgctgagc actaaaattt aatgtaaatg gtgtgttaaa
      181 tcgattgtga ataaccagcg cttccggcag gatacggtcg ccctggtaaa acataaactc
      241 tgttaccccg ttccggtggc agatataacg gcaagtttcg acattgccga taataatttt
      301 ttggagactt tagatggcta ttagcacacc gatgttggtg acattttgtg tctatatctt
      361 tggcatgata ttgattgggt ttatcgcctg gcgatcaacg aaaaactttg acgactatat
      421 tctgggcgga cgtagccttg ggccattcgt gacggcatta tcggcgggtg catcggatat
      481 gagcggctgg ctgttaatgg ggttgccggg cgcggttttt ctttccggga tttccgaaag
      541 ttggatcgcc attggcctga cattaggcgc gtggattaac tggaagctgg tggccgggcg
      601 gttgcgtgtg cataccgaat acaacaataa cgccttaaca ttaccggatt atttcaccgg
      661 gcgttttgaa gataaaagcc gcattttgcg cattatctcc gcgctggtga ttttgctgtt
      721 cttcaccatt tattgcgctt cgggcattgt ggcaggcgcg cgtctgtttg aaagtacctt
      781 tggcatgagc tacgaaacgg ctctgtgggc gggagctgcg gcgacgatcc tttacacctt
      841 tattggcggt ttcctcgcgg tgagctggac tgacactgta caggccagcc tgatgatttt
      901 tgccctgatc ctgacgccgg ttatcgtcat tatcagtgtc ggtggctttg gtgactcgct
      961 ggaagtgatc aaacaaaaga gcatcgaaaa cgtggatatg ctcaaagggc tgaactttgt
     1021 cgccattatc tcgctgatgg ggtgggggct gggttacttc gggcaaccgc acatcctggc
     1081 gcgttttatg gcggcggatt ctcaccacag catcgtccat gcgcgtcgta tcagtatgac
     1141 ctggatgatc ctctgcctgg caggggcggt ggctgtcggc ttctttggta tcgcttactt
     1201 taacgagcac ccgtcggtag ctggtgcggt aaaccagaac gccgagcgcg tgtttatcga
     1261 actggcgcaa attctgttta acccgtggat tgccgggatt ctgctgtcgg cgattctggc
     1321 ggcggtaatg tcaacgttaa gttgccagct gctggtatgt tccagtgcga ttaccgaaga
     1381 tttgtacaaa gcgtttctgc gtaaacatgc cagccagaaa gaactggtgt gggtagggcg
     1441 tgtgatggtg ctggtggtgg cgctggtggc gattgcgttg gcagcgaacc cggaaaaccg
     1501 cgtgctgggc ttagtgagct acgcgtgggc aggctttggc gcggcgtttg gtccggtggt
     1561 gctgttttcg gtgatgtggt cacgcatgac gcgcaacggt gcgctggcgg ggatgatcat
     1621 cggtgcgctg acggtgatcg tctggaaaca gttcggctgg ctgggactgt acgaaattat
     1681 tccgggcttt atctttggca gtattgggat tgtagtgttt agtttgctgg gtaaagcacc
     1741 gtcagcggcg atgcaaaaac gctttgccga ggccgatgcg cactatcatt cggctccgcc
     1801 gtcacggttg caggaaggct aagctgaaga gtaattagtt atcttttgct ttttaacccg
     1861 aatgttccct gccatgcggt ataagttgcc tggcagggag tgttcggggg caattgctat
     1921 ttgcccaccc ggcacagatg aacaaaattg ccgctatcgg taccttcagc ttatgtcctg
     1981 cggctggttg gttgggcgag atagaatgga ttcgacatat tcccgctgtt ttttaaccac
     2041 gccagttttc tcgcaccttg cttggtcact ccgctcgatt tcaggtagga tctggcagta
     2101 gaccactgga agccgcgttt tttactccac ttttcggctc cccctgcgct gttacgctcc
     2161 tcctgggtca tattcgccca ttgtacgatg tctccgaagg tgaacgtgac ccatttctgc
     2221 ggccctgtca gcttcaactc acccagggcg gtaagcccgt cgttcttcag gtaattcctg
     2281 gcagtagttg gcgcgaggtt tctttcctta caccactttt ctaaataacc aaccggtttt
     2341 cgcacatgct tgtttaaggc tgcccactgg cggatatgtt caagggtgaa tttggcatat
     2401 tcatgcggcc tgtttacctt tacctgtccc gcgtagttca ggccgtgatc ggtcaggtaa
     2461 tttctggcat ttttgatatt aaaatgattg cgcgtacacc atcctgaaac tccctgtaga
     2521 gcgtttcgcg cctgctggtc catagccgcc cattttcgca tgtcgtctac agtcaattta
     2581 acatattgct gtggctcgtc cgcctctggc tggtcctcga tggtctgcct ggtgttgttt
     2641 atgtcgtcat ttgcagcatt tggtgggatg ttgcttgtat tacacgattg ctgtaattcc
     2701 acatcgttac gccacgccag cggagacata tttccaaatt ctcgcatccc attgttggta
     2761 aagtgtgttt cttctcttcc gtattgctgg gccggggagt agcgggctgc ttcatgcacc
     2821 ctgttttgac tttcatccgt gagtattggc cgtacggacc agagatcaaa ggttccatat
     2881 tgactgcgtg acatcagggt tgtgacaatg gagtgaaaca gctcaagagt aatatttgta
     2941 ctggtgcaca caccgctctt gctgactgca attttcatga gattgcccgt gttgtcatgc
     3001 tcttgcgtct tcgtgaaggt gatggtgaaa ccggcaacat cgaaggtgaa cggttcagga
     3061 agttgcagat catttgcact taccaccggt tggaactgat gtcgatgcag ttcggttgtc
     3121 atcgcagcag ccagcaagtg aacctcttca ggaaccagtg gagctacagc catgcccgtg
     3181 ctgtagtccg catccctttt gatataatta ttcatctgca ccagaagcgt caaagtttcc
     3241 agacaactac tcgggagttg ttgcggtgga atataggttg ctgcgctcgt attgaggttg
     3301 gatattggtc tcatgtaatg ctctccgtat ccttacaggg ttaatgattc gtattaagtc
     3361 gtagccatcc gcatggctcc gggcgattca gtcggcgcac caggatgtac acttcggatg
     3421 cgttcttgat gtagtaaaca atccgctcat tctggtgtta atggaaacac ccgtaaaaag
     3481 caggaggaat gccag
//
LOCUS       DBFIFI010000304         3487 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.305, whole genome shotgun sequence.
ACCESSION   DBFIFI010000304 DBFIFI010000000
VERSION     DBFIFI010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.305"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            207..452
                     /locus_tag="N7B39_004641"
     CDS             207..452
                     /locus_tag="N7B39_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424468.1"
                     /translation="MTAKHTKKSQSHALDLTEHWLRVSIKIIDRNAGEGYAKAHPELV
                     SAFMTTAAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            449..871
                     /locus_tag="N7B39_004642"
     CDS             449..871
                     /locus_tag="N7B39_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HGU2424469.1"
                     /translation="MTAQIAAYGRLVADPQLKTTSKGTQMTMASMAVPLPCSQADDGT
                     ATMWLSVLAFGRQADALAKHRKGELVSVAGNMQVSQWTGQNGETRQGWQVIADSVISA
                     RTARPGGKKGQQGQATDALNRAKQQAGNDDPYGDNIPF"
     gene            1529..3418
                     /locus_tag="N7B39_004643"
     CDS             1529..3418
                     /locus_tag="N7B39_004643"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HGU2424470.1"
                     /translation="MKTWFSIKAMADVVHVRIYDEIGGYGVKASALTDEINACGNVSE
                     IHLRIHSPGGDIFEGLAIYNALKNHPAKKIVHIEGMAASMASFIAMCGDHIVMPENAM
                     MMIHAPRGVTAGVSGDVRRFADLMDKLGDTMAETYAGRTGRSKQEITAMMEAETWMDG
                     NECKANGFADEVIPAITAMARIESKRIGDFSNMPEKIKSMISQKTGSGEQERLNGIRE
                     LFGTFNGRYNDLAISCLADSECSVENARERLLLAMGKESTPTNKTTPANLYYAYTDNG
                     NITGDAMRQGLNARLGHERAERGNPYAMMSLFDMAQASLTHRGISTGSYGTRSQIVNA
                     AFTHSSSDFTDILAGGAEKSVLAGWEHSGETFRQWTKKGSLSNFREARRVGMNGFSTL
                     NKVPEGAEYKYITTSDRGEPIALATYGNIFSITRQAIINDDLDQLSTVPMAMGRAASR
                     TVGNLVNLVLTGNVKLSDGIALFDKKHSNLIEAGLTTPGLSAARHLMRTQKDKNGEVL
                     NIAPKFLLVPAALEDRALQMINSSAPFGADKNSGIFNPYHKLLDIIVDPRLDDISEKQ
                     WYMLSAQGTDTIEVAYLDGSDEPYLEQQEGFIVDGVAWKVRIDAGVAALDYRGMVKSG
                     GTDSL"
BASE COUNT         1011 a          799 c          928 g          749 t
ORIGIN      
        1 tatgaactgt tcaccaaact attcactgtt cacctttttg attgtttatt gagcttcaag
       61 ggtgaacagt ggtgaacagt tggtgaatag tttttgtgaa actgttcacc ccttaacatt
      121 atgaattaaa agataaaata tcaaaaggtg aacaggtgaa gggttgaaac gcaaaaattt
      181 taatttactg ctgtgagata aagcctatga cagcgaagca cacaaaaaaa tcacaatcgc
      241 acgcccttga tttgacggaa cactggttaa gggtgtcgat aaaaatcatc gaccgcaacg
      301 ccggggaagg atatgcgaaa gcacatcccg aactggttag cgcattcatg acaacggcag
      361 ctgcaaactt tgccacgctg acagaacggg agattgccga agcggaacag gtaacaacca
      421 tcaacgttaa aaccggagag gtggaatcat gacagcacag atagcggctt acgggcggct
      481 ggtggctgac ccacagttaa agaccaccag caagggcaca caaatgacga tggcgagtat
      541 ggctgtcccc ctgccgtgca gccaggcaga tgacggaacg gctacgatgt ggttatccgt
      601 cctggcgttt ggcagacagg ccgacgcact ggcaaaacac cgcaaaggcg agctggtgag
      661 cgtggcgggt aacatgcagg taagccagtg gacaggccag aacggcgaaa cgcggcaggg
      721 ctggcaggtt atcgcagaca gcgtaatcag tgcgagaacg gcgcgaccgg gcggcaaaaa
      781 aggccagcag gggcaggcta ctgacgcact gaacagggca aaacaacagg cggggaatga
      841 tgatccgtac ggggataaca taccgtttta agcaacgagt gacagaagcc ggagaaatcc
      901 ggcttttttg taggtactcc tggtgggggt ggcctgtcca cggggcggag gggcgcggaa
      961 aaaggcgcat tttttgattt ttatggcacc atcaccacca ctataagcta ttgatatgtt
     1021 gagaaataaa aatttttagt gtcgaatcag gttgtttttt gttcatcacc ggaacgttcc
     1081 cgaaaacatt tacaaaaaac aggcgcaaaa aaagcgcccc cgattgctgt taccggaggc
     1141 gcttttacac gacaaaggag tttttatcgc caggatgacg agtcttaata ctgcttcaat
     1201 ggcaaaaatg cgtcaataac tttgcctttc tgagaataat cagaaaaaac ataatctgat
     1261 tttcaggtag aaaatgattt atctattact tttatcgatc aataatgatg cccgttaatc
     1321 aaaacggagg cggatttatg ccagagaaca acaccagaaa gccggataaa agtgccacgg
     1381 tacacataga cgccggaact atggagaaga tcgaacgcta tcagcagttc atcaaagata
     1441 atcacccggg tatgccagtg cccacgaaag gacaaatcac acgcagcgcg gttgaatact
     1501 ggtacagggc aacgttagga gcctggctat gaaaacatgg ttttccatta aggctatggc
     1561 agatgttgta catgtgcgca tttatgacga gatcggcggg tacggtgtaa aggcatcggc
     1621 acttactgac gagatcaacg cgtgcggtaa tgtgtctgaa atccatcttc gcatccattc
     1681 acctggtggc gacatctttg aagggctggc tatctataac gccctgaaaa atcatccggc
     1741 aaagaaaatt gtacacattg aaggcatggc ggcttctatg gcctcgttta ttgccatgtg
     1801 tggcgatcac atcgttatgc ctgaaaacgc gatgatgatg atacatgccc cccgtggtgt
     1861 tactgccgga gtgtcgggcg acgttcgccg ctttgctgac ctgatggaca agctgggcga
     1921 cacgatggcg gaaacctacg ccggaagaac gggcaggagc aaacaggaaa tcaccgccat
     1981 gatggaggcg gaaacctgga tggatggcaa tgagtgtaag gctaacggct tcgcagatga
     2041 ggttataccc gcgattacag caatggcccg aattgaatca aaacgaatcg gagatttttc
     2101 aaatatgccg gaaaaaatta aaagcatgat cagccagaaa actggcagtg gcgaacagga
     2161 acgacttaac ggaatccgtg aattgtttgg caccttcaac ggaagatata acgacctggc
     2221 tataagttgt cttgcggatt cagaatgtag cgttgagaat gcacgcgaac gccttttact
     2281 cgctatgggt aaagaatcaa cgccaacaaa caaaaccacc cccgcaaatc tttactacgc
     2341 gtacacggat aacggcaaca taaccggcga tgcaatgcgc caggggctta atgcgcgtct
     2401 tggtcacgaa cgggccgaac gcggtaatcc ttacgccatg atgagccttt tcgatatggc
     2461 acaggcatca ttaacccatc gtggtataag cacgggcagc tacggcacac gctcgcagat
     2521 agtaaacgcg gcattcaccc acagcagcag cgattttacc gatattcttg ctggtggcgc
     2581 tgaaaaatca gtgcttgcag gctgggagca cagcggcgaa acattccgcc agtggacgaa
     2641 aaaaggttcc ctttcaaact tccgggaagc ccgccgcgtt ggtatgaatg gcttctcaac
     2701 gttaaacaaa gtgccggaag gggcagaata taaatacatc accaccagcg atcgcggtga
     2761 acccatcgcg ctggctactt acgggaatat tttcagcatt acccgccagg cgataatcaa
     2821 tgatgacctt gatcagttat caacggtgcc aatggctatg ggccgtgctg catcaagaac
     2881 ggtgggaaat ctggttaatc tggtgcttac aggcaacgta aaactttctg acggaatagc
     2941 gctgtttgac aaaaaacaca gcaacctgat tgaagcagga ctgacaacac cgggacttag
     3001 tgcagcacgt cacctgatgc gcacacagaa ggacaaaaat ggcgaagtgc tgaatattgc
     3061 gcctaaattc cttttagttc cggcagcact ggaagatcgc gcgttgcaga tgattaactc
     3121 aagcgcacct ttcggggctg ataaaaacag cgggatcttt aacccgtatc acaagctact
     3181 tgatatcatc gtcgatcccc gccttgatga tatcagcgaa aaacaatggt acatgctttc
     3241 cgcacaggga acggacacaa tcgaggtggc ttatcttgat ggcagtgacg agccttacct
     3301 tgaacagcag gaaggtttta tcgttgacgg cgtggcctgg aaagtccgta ttgatgcagg
     3361 tgtggcagct ctggattatc gcggtatggt caaatcaggc gggacagatt cactatgaca
     3421 acaaggcggc accagccgcc ttttttgcgg gtcctcctgg tggggtgggc ctgaacacgg
     3481 ggcgggc
//
LOCUS       DBFIFI010000305         3469 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.306, whole genome shotgun sequence.
ACCESSION   DBFIFI010000305 DBFIFI010000000
VERSION     DBFIFI010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.306"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..1707)
                     /locus_tag="N7B39_004644"
     CDS             complement(<1..1707)
                     /locus_tag="N7B39_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4851/ECs_5259 family protein"
                     /protein_id="HGU2424471.1"
                     /translation="MLVKTPVNPLLQWLNMFFSRRSLSGADGRALYAYRCTDTEYESL
                     AELLRTYAPRSYPRTIFISYSDVLFSIYAAEFIRRTHTVGHPKWDTILDSINWKVPYV
                     HRQKLVNDGIRYWKRKIRNLGQASGYLHTLACEGGLPIRMIENESGYLITYFRRIYQA
                     LRGQSSQYPAAKIAQELGDTIPVTMQNELVYEIAGEFCETLCRLLSEHPPHSSDPVSA
                     LRKLSPDWHLQLPLVLPEANAAEIVRRLLSQSSEIRSASSLQVERIWVDVDDSWYCDA
                     RFRFPATMRTEQLTSLFECHIQPEQTRLIISGKWKNGGARLAMLSRYEQQDWRVELLP
                     IAMQKLSGADAMAEISLSLHEGPILLGHTIPKGGYELTEELPWVFEAMNESESQLKLV
                     GMGSVSSRLNALFISLPKNSHLDISGEGEFDIPRLLKNSERSLTKISGVFSVVLHDGA
                     VCTIRTQQLYDSAIEYYIKSTEVELVKSDYPVHRAWPKIGWKKDLQYGIVPEKELFWR
                     SIRSGNNAWYSVASEMPKGQIEVRRIVNDEVLFSGKVVVLPADFDINIIPESAQQGII
                     MLS"
     gene            1919..>3469
                     /gene="zorA2"
                     /locus_tag="N7B39_004645"
     CDS             1919..>3469
                     /gene="zorA2"
                     /locus_tag="N7B39_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II Zorya anti-phage system protein ZorA2"
                     /protein_id="HGU2424472.1"
                     /translation="MLAQLFEQLFQSIDSTLITNIFIWAVIFVFLSAWWCDKKNIHSK
                     FREYAPTLMGALGILGTFIGIIIGLLNFNTESIDTSIPVLLGGLKTAFITSIVGMFFA
                     ILFNGMDAFFFANKRSALAENNPESVTPEHIYHELKEQNQTLTKLVSGINGDSEGSLI
                     AQIKLLRTEISDSSQAQLANHTHFSNKLWEQLEQFADLMAKGATEQIIDALRQVIIDF
                     NQNLTEQFGENFKALDASVKKLVEWQGNYKTQIEQMSEQYQQSVESLVETKTAVAGIW
                     EECKEIPLAMSELREVLQVNQHQISELSRHLETFVAIRDKATTVLPEIQNKMAEVGEL
                     LKSGAANVSASLEQTSQQILLNADSMRVALDEGTEGFRQSVTQTQQAFASMAHDVSNS
                     SETLTSTLGETITEMKQSGEEFLKSLESHSKELHRNMEQNTTNVIDMFSKTGEKINHQ
                     LSSNADNMFDSIQTSFDKASAGLTSQVRESIEKFALSINEQLHAFEQATEREMNREMQ
                     SLGNALLSI"
BASE COUNT         1090 a          727 c          671 g          981 t
ORIGIN      
        1 agaaagcata ataatgcctt gctgagcact ctcaggtata atattaatat cgaagtctgc
       61 tggtaaaact acaactttac cgctaaataa aacctcatca ttaactattc gccggacttc
      121 tatctgtcct tttggcattt ctgatgcgac agaataccag gcattattac ctgaacgaat
      181 ggaacgccaa aaaagttctt tctccggtac aatgccatat tgcagatcct ttttccaacc
      241 gatcttcggc caggctcgat ggacaggata atccgattta accagttcaa cttctgtcga
      301 tttaatataa tattcaatcg cagaatcata aagttgctgg gtgcgaattg tacaaacagc
      361 accatcatgt aaaacaacgc taaatactcc acttatttta gttaagctgc gttcactgtt
      421 tttcagcaac cttgggatat caaactcacc ttctccacta atatctaaat ggctattttt
      481 cggtagcgaa ataaacagag catttagcct ggaactcaca gaccccatac caacaagttt
      541 tagctgcgat tcactctcat tcatcgcttc aaaaacccag ggaagttctt cagtaagttc
      601 ataaccgcct tttggaattg tgtgacctaa cagaattgga ccttcatgaa gcgacaatga
      661 gatttcggcc attgcatctg caccagagag tttttgcatc gcaataggta ataactcgac
      721 ccgccaatct tgctgctcat aacggcttag cattgccaac cttgccccgc catttttcca
      781 ttttcctgaa atgatgagtc gggtctgctc cggctgaata tggcattcaa acaaagaggt
      841 taactgttct gtacgcattg tggccgggaa tcggaaccgg gcatcgcaat accaactgtc
      901 atcaacatcg acccagattc tttcaacctg aagactactt gcactgcgta tttcagaaga
      961 ttgagaaagt aatcgcctta caatctctgc agcattcgct tcggggagaa cgagtggaag
     1021 ttgaagatgc cagtctggag agagcttacg taatgcagaa acgggatcac tgctatgagg
     1081 aggatgctca ctaagtaatc ggcagagagt ctcgcagaat tcaccggcta tttcatagac
     1141 cagttcgttt tgcattgtga caggaatcgt atcgcctaat tcttgtgcaa ttttagcggc
     1201 aggatattga gatgattgcc cacgaagcgc ctgatatatc cttctgaaat aggtaatcaa
     1261 ataaccgctc tcattttcaa tcatgcggat aggcaaacca ccttcacaag ccagtgtatg
     1321 cagataaccc gaagcttgtc ccaggtttct tatctttctt ttccagtagc gaatgccatc
     1381 attaaccagc ttctgtcgat gaacatacgg aactttccag ttaatagaat ctaaaattgt
     1441 gtcccattta gggtgtccga ccgtatgagt ccggcggata aactctgcgg catatatgct
     1501 aaatagaaca tcgctatagg aaatgaatat cgttcttgga taactacggg gggcatatgt
     1561 acgcagtagt tctgccaaac tttcgtactc tgtatcagta caacggtaag catataacgc
     1621 ccgtccatca gctccggata atgaacggcg actaaaaaac atgttaagcc attgcaaaag
     1681 tgggttaact ggagttttca caagcaacct gccttccttt gatacaattc gtaacaggtt
     1741 actatcatca taaaaaagct caacccgatg aactcgctaa aaatgagaca aatcatttat
     1801 atctcgaaaa aacttgttac aatcatgagc gctacaccga acttaaccat ataaattatg
     1861 tgtgttttgt ttatttttta aacgattaca actatccatt atttacacag gtatcaaaat
     1921 gttagcgcag ctttttgagc agttgtttca atcgatagac tctacactga tcaccaatat
     1981 tttcatctgg gctgttatat tcgtattttt atcagcgtgg tggtgtgaca aaaaaaatat
     2041 acatagtaag tttagagaat atgctccaac cttaatgggg gcattaggta ttctgggtac
     2101 tttcattggt attattattg gtttactcaa ttttaatacc gaaagtattg ataccagcat
     2161 ccccgtatta ttaggtggcc taaaaacagc attcattaca agcattgtag gtatgttttt
     2221 tgccatttta tttaatggaa tggatgcttt cttttttgcc aataaacgaa gtgcgttagc
     2281 agaaaataac cctgaatctg ttacacctga acatatctat catgaattaa aagagcagaa
     2341 ccagactctg actaaattag tctcgggtat taacggtgat agtgaaggtt ctcttattgc
     2401 tcaaataaaa ttactacgta ctgagattag cgattcctcg caggcacaat tagctaatca
     2461 cactcatttc agtaataagc tttgggaaca acttgaacaa tttgcagatc taatggcaaa
     2521 aggtgctaca gaacaaatta ttgatgcttt gcgacaagtc attattgatt ttaatcaaaa
     2581 tttaactgaa cagtttggtg aaaactttaa agctcttgat gcctctgtaa aaaaacttgt
     2641 tgagtggcag ggaaattata aaacgcaaat tgagcagatg tcagaacaat atcaacaaag
     2701 tgtcgagtcc ctggttgaaa caaaaactgc ggttgcaggg atttgggaag aatgtaaaga
     2761 aattcctctg gctatgtctg aactgcgtga agtgcttcag gtgaaccaac atcaaatcag
     2821 cgaactctcc cgccatttag aaacctttgt cgccatccgc gataaagcta caaccgtatt
     2881 acctgaaata cagaacaaaa tggctgaagt gggtgaactg ctgaaatccg gagctgcaaa
     2941 tgttagtgca tctcttgagc aaaccagcca gcaaatactt cttaatgcag attcaatgcg
     3001 cgttgccctg gatgaaggta ccgaaggatt cagacaatcg gttacccaaa cacaacaagc
     3061 atttgcctcg atggcacatg atgtcagcaa ttcctccgaa accctaacca gcacgttagg
     3121 tgaaacaatt actgaaatga aacaaagtgg tgaagaattc ctgaaatcac tagagtcgca
     3181 ctcgaaagaa ttgcatagaa atatggaaca aaatacgacg aatgtgattg atatgttcag
     3241 taagactggt gaaaagatta accatcaact atccagtaat gccgataata tgtttgattc
     3301 aatccagaca tcatttgata aggcaagtgc agggctgact tctcaagtca gagaatcaat
     3361 tgaaaaattt gctctatcca tcaacgagca gttacatgct tttgagcaag caactgaacg
     3421 tgaaatgaac cgtgaaatgc aatcattagg taatgctctg ctttcaatc
//
LOCUS       DBFIFI010000306         3412 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.307, whole genome shotgun sequence.
ACCESSION   DBFIFI010000306 DBFIFI010000000
VERSION     DBFIFI010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.307"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(12..422)
                     /locus_tag="N7B39_004646"
     CDS             complement(12..422)
                     /locus_tag="N7B39_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HGU2424473.1"
                     /translation="MTIKEKFSQKYPHASFCTFGDSAALADHLATLIATGVKTASCGS
                     LAGCIEDNAFPMIGEYKIVENSRGEPVCVIRVIGLHLLRFSDVTAELARKEGEGDLSL
                     EYWRNEHRRFFQAEGSYSPEMDVIFEEYALIDVV"
     gene            complement(721..2103)
                     /gene="yahJ"
                     /locus_tag="N7B39_004647"
     CDS             complement(721..2103)
                     /gene="yahJ"
                     /locus_tag="N7B39_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HGU2424474.1"
                     /translation="MKENNSRREFLSQSGKMVTAAALFGTSVPLAHAAVSGTTNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQNGKIVALRENKQHPDAT
                     LPHYDAGDKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDELAHRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            complement(2113..3063)
                     /gene="yahI"
                     /locus_tag="N7B39_004648"
     CDS             complement(2113..3063)
                     /gene="yahI"
                     /locus_tag="N7B39_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HGU2424475.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDELQKANPDWRFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTDAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPEKQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            complement(3113..3280)
                     /locus_tag="N7B39_004649"
                     /pseudo
     CDS             complement(3113..3280)
                     /locus_tag="N7B39_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3238..3411)
                     /locus_tag="N7B39_004650"
                     /pseudo
     CDS             complement(3238..3411)
                     /locus_tag="N7B39_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          740 a          946 c          869 g          857 t
ORIGIN      
        1 taaatagtta tctacacaac gtcaataagg gcatattctt caaagataac gtccatttct
       61 ggtgaatagc ttccttctgc ctggaaaaaa cgacggtgtt cattacgcca atattcaaga
      121 ctgaggtcac cttcaccttc cttacgggca agctccgcgg tgacatcaga aaagcgcagt
      181 aaatgcaggc caatcactct gataacacat acaggctcac cacggctatt ttctacaatc
      241 ttatactcac caatcatcgg aaatgcgttg tcttcaatac atcccgccag cgaaccacag
      301 gaagctgttt taacaccagt cgcaatcaac gttgcgaggt gatcggccaa cgcggctgaa
      361 tcgccaaaag tacaaaagga agcatgggga tatttttgtg agaatttttc ttttatggtc
      421 atggcaatat ttttgcgatt acggaaaaac aaatcacatc ttaattactg ttggtaacca
      481 tcagcaagag tatcattggt ttttcgatac tgaatctttt ttatccgaag caggtcacat
      541 ttatggcctg ataaatacct ttgttaaaac tcacttttgt gtatttctct caaataatcc
      601 cctggaaatt atgccgggag attataaagt tcaggatcat tcattagcaa accattcccg
      661 ccccactccc tggggcttaa atctcttact attcaccgtt gcaggcgttt caaatgcgca
      721 tcaacctgcc acactccccc acaccagttg ccctttatgg aacgttgcgg ttctcggcga
      781 gatacgcgcc accgcctcgg cggaacagga ggcgtccacc agcacaaagc tggcgtcatc
      841 ctgcgctttt ggccatacac gctcgccttt ttcattcagc ggcaatacat cgccagtggc
      901 tagaaacagc gaacgggaga ggttctgttc gttaggacga atatagagct gcgcgtagag
      961 attggctttt tccagcatgt cgcccagacc ataaggcgac cagtggtcga taacgctgtc
     1021 agtgccagtc atcactttta cgcctttgtc gtgcaactgt ttgagcggca tatgcagcgt
     1081 gccaatcggc accgtcgagg cgatagaaat ttgctgcgcc gccatgcgat gcgccagttc
     1141 atctacctgt tgctcgttga gcgtagccag cgcaaaggcg tggctgatgg tcagcttacc
     1201 tttcagttga ggcgttttct ctaccgtttc aaccatataa ttgatggctg ccacgcccga
     1261 cggagtggtt tcgtgcaggt gaatatcgac gcctttgtcg tagtccagcg caatctggaa
     1321 catggtgtcg agggattttt ccatcgcgcc atcgacactg gtcgggtcca gcccgccgac
     1381 gtaatgcgca cccgcctgca ttgcttcacg cattaagggt tccgatttcg acagcagcaa
     1441 accgtgctgc gggaaggcga cgatttcaca ctcaaagccc gcctgacgtc gcgccagcac
     1501 cgcctgcaaa ttttgcagat ttttcaggcc ggaaaccggt tcgatattgc agtggctgcg
     1561 ggcaatggtg gtgcctttcg actgcaataa atcaatcagt ttttctgccc gctcctgagt
     1621 gtacggttgc agctccggca gcattttctg ctcgagtttg atcatgtcct ggatggtggt
     1681 gcctgccggg cgattgagcg agcgccacgg cccgccgtaa aacgttttgt cgagatgaat
     1741 atgcatgtcg cgggtggtgg gcagcatcag cttatcgcca gcgtcatagt gcggcagcgt
     1801 ggcatccggg tgctgcttat tctcgcgcag cgcgacaatt ttgccgttct gaatctccac
     1861 ggtctggcgc gccgtgcggg tctgcaccgc cacgccattt tcgtagtcaa aaccggtttc
     1921 cagcagcacg ttgtcgagat agtaatgcgg gtcagtgatt ttcatggtgt tgttcgcttc
     1981 gcagtttgtg gtgccagaga ccgccgcatg ggcgagcggc acagaggtac caaacagcgc
     2041 ggcggcggtg accatcttac cgctctggct cagaaattca cggcggctat tattttcttt
     2101 catcttacgt ccttacgttt taataatgtg ggtgcccgtt tcaccgcgca gcgccgctgg
     2161 caggcattcc ggcgtggtga taatcacttc tttaccgccc tgttccagga acgtcaggct
     2221 ggcgatgatt tttggcaaca tgctgccggg cgggaaatgt ccttcctgca tatagcgagt
     2281 catggtggca atatccaccc gatccagcgc ctgcttctcg ggtttaccaa agtggataca
     2341 gactttttcg acgccggtgg tgatcaccag aatgtcggcg tgaatttcac gggccagcag
     2401 cgcggtagag agatctttgt cgataaccgc gtccacgctt tggtaatctc ccgcgtcagt
     2461 acgcaccaca ggaattccgc cgccgcccgc gccaatcacc acaaagcctt gttggatcag
     2521 cgccttaatg gcgggtgctt cgacaatacg tttcggttcc ggcgaggcga ccacgcggcg
     2581 atagccccgt ccagcatctt caacaaaacg ccagtcaggg tttgcctttt gtaattcgtc
     2641 acgctggctt tcactgaaga atgcaccaat gggcttggtg ggatgggcaa aacctggatc
     2701 gtttttatcc acttccacct gagtcaccac ggtgacggct tttttctcgc cgtgacgcgc
     2761 cagccggttg ttcagcgcct gttggatcag gtagccaatg ccgccctgcg tatctgccac
     2821 acagttcgcc agcggcgtta agggcagccc ttcgcgctcg tgggcaatct ccgcacggcg
     2881 taaatccagc ccgacctgcg gcccgttacc gtgggtcagc acaatgtcgt aatcggaagc
     2941 cagcatttcc agcaccgtat cggccacggc tttcaccgcc tccgcctgat gctcaatcga
     3001 ctggctggcg ttatctttga taatgctgtt gccaccaatg gcaacgacca caagttcttt
     3061 catgttgtta tgtccgctac agagggtgat ggggttatga ccaacgccag actgcctgat
     3121 gcgctacgct taccaggcct acaaattctc gtgccattcg taggccggat aaggcgttca
     3181 cgccgcatcc ggcaatggtg acaaatgcct gatgcgctac gcttatcagg cctacaattc
     3241 tcgtgccatt cgtaggccgg ataaggcgtt cacgccgcat ccggcaatgg tgacaaatgc
     3301 ctgatgcgct acgcttatca ggcctacaga ttcttgcgcc attcgtaggc cggataaggc
     3361 gttcacgccg catccggcaa tggtgataaa tgcctgatgc gctacgctta tc
//
LOCUS       DBFIFI010000307         3401 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.308, whole genome shotgun sequence.
ACCESSION   DBFIFI010000307 DBFIFI010000000
VERSION     DBFIFI010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.308"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..901)
                     /locus_tag="N7B39_004651"
     CDS             complement(<1..901)
                     /locus_tag="N7B39_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HGU2424476.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTRTPAPVGIFGPGWKAPSDI
                     RLQLRDDALVLNDNGGRSIHFEPLLPGEAVYSRSESMWLVRGGKAAQPDGHTLARLWG
                     ALPPDIRLSPHLYLATNSAQGPWWILGWSERVPGAEDVLPAPLPPYRVLTGMADRFGR
                     TLTYRREAAGDLAGEITGVTDGAGREFRLVLTTQAQRAEEARKQHTASLSSPDTPRPL
                     SDSAFPDTLPGTEYGPDRGIRLSA"
     gene            complement(968..3076)
                     /locus_tag="N7B39_004652"
     CDS             complement(968..3076)
                     /locus_tag="N7B39_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HGU2424477.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILATILKENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSIDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     RGSGTTLNNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKNYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAETIGNNQMIAVTNNQIQTVGVNQIETVGSNQIINVGSVQVETIGLVRA
                     LTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLRVGLGYDVKVGNNVTFTVGKTKKDD
                     TGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGTKIHLQGKEQVNGDSLLINWNCAA
                     SKSPPKTPDEKQDTPDMREY"
BASE COUNT          710 a         1023 c          890 g          778 t
ORIGIN      
        1 ccgccgaaag gcggatacct ctgtcgggac cgtattcggt accgggcagt gtgtcgggga
       61 acgctgagtc tgaaagaggg cggggggtgt caggggaaga taaagaagcg gtgtgctgtt
      121 tacgggcctc ttccgcccgc tgcgcctgcg tggtcagcac cagacggaac tcccgcccgg
      181 caccgtccgt cacgccggtg atttccccgg ccaggtcacc ggcggcctca cgcctgtacg
      241 tcagcgtccg cccgaagcgg tccgccatcc cggtaagcac ccggtacggc ggcagcggcg
      301 ctggcagtac gtcctcagca cccggcaccc gctctgacca ccccagtatc caccacggcc
      361 cctgtgcgct gttggtcgcc aggtaaagat gcgggcttaa ccggatatcc ggcggcagcg
      421 ccccccacag ccgcgccagc gtgtggccgt ccggctgcgc tgccttacca ccgcgcacca
      481 gccacattga ctcgctgcgg ctgtacaccg cctcccccgg cagcagcggc tcaaagtgaa
      541 tgctccgccc gccgttgtca ttgagtacca gtgcatcatc gcgtagctgt aagcggatat
      601 cagaaggcgc tttccagccg gggccaaaaa tccccaccgg cgcaggcgtc cgggtccggt
      661 agctgctgta ggtgcgggag agaatgaacg gcagcgggcc gggcagcgca aggtccgtct
      721 cgccgggcag caccttcgcc cccagcagcg gatttaccgg gttgcccgaa gtcatcccgc
      781 ccgggcacac cgagcacgcc acgccagttg gcgcgccaat tcttacacct gccgaaccct
      841 ggacaatggg accgccatac tgagtcatat ctccctgacg cgccgctggt tttccgctca
      901 tgattcaatt ccttcttctg tttattttta atgcttgttt gttactgaat accgaacagg
      961 tacgggatca gtactctctc atatccggcg tatcctgctt ttcatcaggg gtcttcggtg
     1021 gggatttcga ggctgcacag ttccagttaa tcaacagtga gtcaccatta acctgctcct
     1081 taccctgcaa atgaattttt gtgccgttga gaaaaatttg tccgtccttc gtcagcacca
     1141 gccttgcctt accacagcag agctccagat gctcaccggc ggagtaaatc gcggtctgcc
     1201 cggtatcatc ctttttcgtt ttaccaacgg tgaaggtaac gttatttccg actttgacgt
     1261 cataacccag cccgaccctc aacgatttat gcaaaccgat ctgcgaggac tgcatcagag
     1321 ccaccgaagt gttcataatg ccacctaccg tcgtctggta cgccacgccc acggtcagcg
     1381 cacgaacaag tccaatcgtt tccacctgaa cagaacccac gttaatgatc tggttactgc
     1441 ccaccgtctc tatctggtta acgcccaccg tctgtatctg attgttggta accgcaatca
     1501 tctgattgtt gccaatggtc tcagcatggt tgtttatcac atccgtggtg cggttattca
     1561 gcacctcggt gttcatgttc ttctgcgcgt ggatgtagac ctgttctttc cctgtcgcat
     1621 cgtcaaactt cagttcatta aacccgctgc ccttatagtt tttcgaacga atggtcatct
     1681 gcgtcttcgt ccccggcagg ctgccgggtg tgcggttttc ctggtggtag gtacgcccca
     1741 taataatcgg ctggtccgga tcgccgttga ggaagtccac aatcacctcc tgacccacac
     1801 gcggtatcgc caggttgcca aagccggtgc ctgcccacgc ctgtgccaca cggatccagc
     1861 atgaactgtc ctggtttgac gggttatagc ggtcccagtt aaatttcacc cgcacgcgac
     1921 catgttcgtc gcagaagatt tcctcgcctg ccggtcccgt cacgacggcg ctctgcgggc
     1981 catccaccag cggtttcagc aacggctgtg gtcgccatgt ccggtctgcc ggtattaccg
     2041 caaagtggtt attcagcgtg gtacctgaac ccctgcgtcc cggcaccgcc tgtggctgct
     2101 cgccgtgcag ttcacttgcc accacctgcc attcccggtt caggttcgcc tgcggatgcc
     2161 ccgtcagcac aattcgccgt cccggccata tttccggaga acggcttgtt ccgcgcgcca
     2221 cttctgcgtt gttgcgccag ccatccatct gccagcgggc aaagttctgc ccgtgggcac
     2281 ccttgaaacg tccggggtag tcatacactt catactgtgt gcgctggtaa tcctggtact
     2341 ggccttcctg atcaaaacgc cctgcccagc cgggtcgttt aaaggtgtag tccttggtca
     2401 ccacggaaga agggcggatt tgtgcgctgt agcggaactg gctgatgcat agggtgctca
     2461 cctcggtacg ggtgttcggg ttccagggga tctcaaagga ctccggcaga taacgcacgg
     2521 tgtcgcacag gaccaggctc tggtcgatac ttttttgcgc gtgctcctca taaaagaaga
     2581 tgccttcctc cgccgccatc cggcacagga aatcgtaatc agtttcgccg tactggacac
     2641 aaaactcacg ggaaggatgc ggctcgctga acagcggact ccactcggtc acaccgtttt
     2701 ctttcaggat cgtagcgagt atgctttcga tgtcctcatt ctggaagata cggaagttct
     2761 ggcgcagccc tgtgcgccac agcggcgggc acaccttcat gctgtacagc atctggtttt
     2821 tgtcgttctc ccccagttca aaccaggtca ccacaccttt cacccggcgc tgtacgtcat
     2881 cgccctgcca taccgtcagg taggccattt tgtccagcac ctgcgcaaat tcaagggaaa
     2941 gaaactgctg actgaccaga gaaaggtcga gggaaaaaag tgaagagagt gactggttca
     3001 gatgaaagga gaccaccgca aacgcgtccg gtggcaggcc gtccacttcc agcgtgaaac
     3061 gtaatccggt tgacatagca acctccgtga aaatgaataa taatccgcac atattgcatg
     3121 tacaacatgt cgggcggatg aaataagtga gtcgacatct ggcgttaccg gggaatatca
     3181 gaaaaatgtc atggttatgt gatgatttag tctgggattt tacttcagag aaacgggtgt
     3241 ctgtttagtc aacaaaataa tcacaaacgc aacggatggg ttctcgacgt tattcacgac
     3301 aggctcgtac attacagcga gtaataaaaa acctgatatg caaagtcaaa ccaatattca
     3361 taatgtcttt ttattcacaa caataaataa tttaataaca a
//
LOCUS       DBFIFI010000308         3386 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.309, whole genome shotgun sequence.
ACCESSION   DBFIFI010000308 DBFIFI010000000
VERSION     DBFIFI010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.309"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..447)
                     /locus_tag="N7B39_004653"
     CDS             complement(<1..447)
                     /locus_tag="N7B39_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HGU2424478.1"
                     /translation="MLFFLSFRGDRGLFIKDIVLKMLTPNRLLCVILLIAGYQLVSVI
                     HHFWLTQAASVPGLSRVSAPETAVTGDQTEERFVFTLFGRASPLSSEGRAQETMPSLS
                     DDLLSGEDLDVRGILYSSVAEHSVAIFAHNNRQFSLSVGEKVPSYDA"
     gene            complement(534..3230)
                     /gene="stcE"
                     /locus_tag="N7B39_004654"
     CDS             complement(534..3230)
                     /gene="stcE"
                     /locus_tag="N7B39_004654"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M66 family glycomucinase StcE"
                     /protein_id="HGU2424479.1"
                     /translation="MNTKMNERWRTPMKLKYLSCTILAPLAIGVFSATAADNNSAIYF
                     NTSQPINDLQGSLAAEVKFAQSQILPAHPKEGDSQPHLTSLRKSLLLVRPVKADDKTP
                     VQVEARDDNNKILGTLTLYPPSSLPDTIYHLDGVPEGGIDFTPHNGTKKIINTVAEVN
                     KLSDASGSSIHSHLTNNALVEIHTANGRWVRDIYLPQGPDLEGKMVRFVSSAGYSSTV
                     FYGDRKVTLSVGNTLLFKYVNGQWFRSGELENNRITYAQHIWSAELPAHWIVPGLNLV
                     IKQGNLSGRLNDIKIGAPGELLLHTIDIGMLTTPRDRFDFAKDKEAHREYFQTIPVSR
                     MIVNNYAPLHLKEVMLPTGELLTDMDPGNGGWHSGTMRQRIGKELVSHGIDNANYGLN
                     STAGLGENSHPYVVAQLAAHNSRGNYANGIQVHGGSGGGGIVTLDSTLGNEFSHEVGH
                     NYGLGHYVDGFKGSVHRSAENNNSTWGWDGDKKRFIPNFYPSQTNEKSCLNNQCQEPF
                     DGHKFGFDAMAGGSPFSAANRFTMYTPNSSAIIQRFFENKAVFDSRSSTGFSKWNADT
                     QEMEPYEHTIDRAEQITASVNELSESKMAELMAEYAVVKVHMWNGNWTRNIYIPTASA
                     DNRGSILTINHEAGYNSYLFINGDEKVVSQGYKKSFVSDGQFWKERDVVDTREARKPE
                     QFGVPVTTLVGYYDPEGTLSSYIYPAMYGAYGFTYSDDSQNLSDNDCQLQVDTKEGQL
                     RFRLANHRANNTVMNKFHINVPTESQPTQATLVCNNKILDTKSLTPAPEGLTYTVNGQ
                     ALPAKENEGCIVSVNSGKRYCLPVGQRSGYSLPDWIVGQEVYVDSGAKAKVLLSDWDN
                     LSYNRIGEFVGNVNPADMKKVKAWNGQYLDFSKPRSMRVVYK"
BASE COUNT          912 a          826 c          680 g          968 t
ORIGIN      
        1 agcatcgtag ctgggtactt tttcgccgac gctcagactg aactgtctgt tattatgtgc
       61 aaatatggca acggaatgct ctgcaaccga actataaagt atacctctca cgtcaagatc
      121 ctcccctgaa agcagatcat ctgacaggga aggcattgtt tcctgcgctc tcccctccga
      181 tgatagtggg gatgccctgc cgaataacgt aaaaacaaaa cgttcttcag tttgatcacc
      241 agttaccgct gtttccggcg cgctaacacg ggataagccg ggcaccgatg ctgcctgagt
      301 cagccagaaa tgatggataa ccgacaccag ctgatatccg gcaataagta aaattacgca
      361 caatagccgg tttggcgtca gcattttaag tacgatatct tttataaata agccccggtc
      421 accccggaat gatagaaaga acaacataat aaaacgctgg acgctacttt atgttttgat
      481 taacgggacg aatgtgttac taatgcggcc gaagtggccg caccgtctca ggattattta
      541 tatacaaccc tcattgacct aggtttactg aagtccaaat actgtccgtt ccaggcttta
      601 acttttttca tatcagctgg gttcacatta cctacaaact caccaatcct gttataggac
      661 aggttatccc agtcagaaag cagcactttc gctttagccc cgctgtcgac atagacttcc
      721 tgcccaacaa tccagtcagg aaggctatat cctgaccgtt gaccaaccgg caaacagtaa
      781 cgtttacctg aattcacgga cacgatgcat ccctcgtttt cttttgctgg aagtgcctgc
      841 ccatttacag tataggtaag tccttctggc gcaggtgtga gcgatttggt atccagtatc
      901 ttgttattgc aaaccaatgt ggcctgtgtg ggctgacttt ctgttggcac gttaatatgg
      961 aacttattca ttacagtgtt gttagcccgg tgattagcca gtctgaatcg caactgccct
     1021 tctttcgtat ccacctgcag ctggcagtcg ttatcggata gattctgact atcatcggaa
     1081 taagtgaagc cataggcacc atacatcgca ggatagatgt agcttgacag cgtgccttcc
     1141 ggatcgtaat accccaccag ggtcgtcaca ggaacaccaa actgctctgg cttacgcgct
     1201 tcacgagtat caaccacatc acgttctttc cagaactgac catcggaaac aaagctcttt
     1261 ttatacccct gggaaacgac cttttcgtca ccatttataa acagataact attataaccg
     1321 gcctcatggt tgatggtcag gatactgcct ctattatctg cggaggctgt agggatatag
     1381 atgtttcttg tccagttacc gttccacata tgcactttga cgacagcgta ctctgccatc
     1441 agctcagcca ttttgctttc acttagctca ttgactgaag ccgtaatctg ctccgcacgg
     1501 tcaatggtgt gttcatacgg ttccatttcc tgcgtatctg cattccactt gctgaagccg
     1561 gtggaggaac ggctatcgaa cacagcttta ttttcaaaaa aacgctggat gatagccgat
     1621 gaattcggag tatacattgt gaaacggttt gcagcagaga aagggctgcc tcccgccatg
     1681 gcgtcaaaac caaatttgtg tccatcaaac ggttcttgac actgattatt cagacaactc
     1741 ttttcatttg tttgagacgg ataaaagtta ggaataaacc gttttttatc accatcccat
     1801 ccccaagttg agttgttatt ttctgcacta cgatgtacag aacccttgaa accatctaca
     1861 taatgaccaa gaccataatt atgaccaact tcatgactga actcattccc caatgtggaa
     1921 tctaaagtaa caattccccc acctccggag ccaccatgaa cctggatgcc attagcataa
     1981 ttaccgcggc tattatgtgc cgctaattgc gcaactacat atggatgact attctcccct
     2041 aagcctgcgg tactatttaa accatagtta gcattatcaa tgccatgcga aaccaattct
     2101 ttacctattc tttgacgcat tgtaccacta tgccacccac catttcctgg atccatatct
     2161 gtcaataact ctccggttgg taacataact tcctttaggt gtagaggcgc ataattatta
     2221 acaatcatac gacttacagg aatggtctgg aaatattccc tatgtgcttc tttgtctttg
     2281 gcaaaatcaa agcgatcccg gggagtggtc aacatcccga tatcaattgt atgcaacaac
     2341 agctcacccg gtgctccaat cttgatatca tttaggcgac cgctcagatt gccctgttta
     2401 atcaccaagt ttaaaccagg cacgatccag tgcgcaggca gttcagcact ccaaatatgc
     2461 tgagcataag tgattcgatt attctccagt tcaccggagc ggaaccactg accatttaca
     2521 tatttgaaca gaagagtgtt acccaccgag agtgtgactt ttcgatcacc ataaaaaacc
     2581 gttgaactat agcctgcaga cgaaacaaag cgaaccatct taccttcaag gtcgggtccc
     2641 tgcggcagat aaatgtctct tacccaacga ccatttgcag tatggatctc caccagtgca
     2701 ttatttgtta gatggctatg aatagaactc ccgctggcat cactgagttt gtttacttca
     2761 gccaccgtat taatgatctt tttcgttcca ttatgaggtg tgaaatcgat accaccttcc
     2821 ggaacaccat ccagatggta gattgtatcc ggtagtgatg aaggaggata aagggttaac
     2881 gtaccgagaa ttttattatt atcatcgcgg gcttccacct gaacaggtgt tttatcatca
     2941 gctttcaccg gacggacaag cagcagactt ttccgcaggc tggtcagatg tggttgacta
     3001 tccccttctt taggatgggc gggtaaaatc tggctttgtg caaatttcac ctctgcggcc
     3061 aacgaaccct gcagatcatt tataggctgg gaggtattga aataaatggc tgaattatta
     3121 tcagcagctg ttgcagaaaa taccccaatc gccagagggg caaggatcgt acatgacaga
     3181 tactttaatt tcatcggtgt tctccatctc tcattcattt tagtgttcat gcagccggat
     3241 ataacagtat ttctatattt tattttttca ggagcgactt attcactagt cgttggttat
     3301 ttttataaca tatttacacc tgtttcgtaa atatatcctg accaaagaat taactcttat
     3361 aacgtccttt aacttatttt attatt
//
LOCUS       DBFIFI010000309         3332 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.310, whole genome shotgun sequence.
ACCESSION   DBFIFI010000309 DBFIFI010000000
VERSION     DBFIFI010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.310"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            231..1163
                     /gene="rfaD"
                     /locus_tag="N7B39_004655"
     CDS             231..1163
                     /gene="rfaD"
                     /locus_tag="N7B39_004655"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HGU2424480.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LNIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            1173..2219
                     /gene="rfaF"
                     /locus_tag="N7B39_004656"
     CDS             1173..2219
                     /gene="rfaF"
                     /locus_tag="N7B39_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HGU2424481.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLKELNALLLQEEA"
     gene            2223..3215
                     /gene="rfaC"
                     /locus_tag="N7B39_004657"
     CDS             2223..3215
                     /gene="rfaC"
                     /locus_tag="N7B39_004657"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HGU2424482.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPEIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGENQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            3212..>3332
                     /locus_tag="N7B39_004658"
     CDS             3212..>3332
                     /locus_tag="N7B39_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424483.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKH"
BASE COUNT          845 a          811 c          871 g          805 t
ORIGIN      
        1 attcaaatgc ttccgttatt tatcatacat cggaatattg atactaaagc actattcaca
       61 tgcaaaacca acatccgcca tgaaggacta gctaaaaccc aaactagttt gttgcaatta
      121 gcatccttgc acctctatgt aaagggctga agggattcgg atgtgatggt atgattacag
      181 acattcgtgt ctgagattgt ctctgactcc ataattcgaa ggttacagtt atgatcatcg
      241 ttaccggcgg cgcgggcttt atcggcagca acatcgttaa agccctgaat gataaaggca
      301 tcaccgatat tctggtggtg gacaacctga aagacggcac caagtttgtg aacctggtgg
      361 atctgaatat cgcagactat atggataagg aagacttcct gatccagatt atggctggcg
      421 aagagttcgg cgatgtcgaa gcgattttcc acgaaggcgc gtgctcttcc accaccgagt
      481 gggacggcaa gtatatgatg gataacaact atcaatactc caaggagctg ctgcactact
      541 gcctggagcg tgaaatcccg ttcctgtacg cttcttccgc agccacctac ggcggacgca
      601 cctccgactt tattgaatcc cgcgagtacg aaaaaccgtt gaacgtctac ggttactcaa
      661 aattcctgtt tgatgaatat gttcgccaaa tcctgccgga agcgaactcg cagattgttg
      721 gcttccgcta tttcaacgtt tatggaccgc gtgaaggcca taaaggcagc atggcgagcg
      781 tcgctttcca tctcaacacc cagcttaata acggtgaatc accgaagctg ttcgaaggta
      841 gcgagaactt taaacgcgac ttcgtctatg ttggcgatgt ggcagatgta aacctgtggt
      901 tcctggaaaa tggcgtttcc ggcatcttca acctcggcac cggtcgtgcg gaatccttcc
      961 aggctgtagc tgatgctacg ctggcttatc acaagaaagg ccagatcgaa tacattccgt
     1021 tcccggataa gctgaaaggc cgctaccagg cgttcactca ggcagatctg acaaatctgc
     1081 gcgcggcggg ttacgacaaa ccgttcaaaa ccgttgctga aggtgtaacg gaatacatgg
     1141 cctggctgaa tcgcgacgca taagagctct gcatgaaaat actggtgatc ggcccgtctt
     1201 gggttggcga catgatgatg tcgcaaagtc tctatcgcac gctccaggcg cgctatcccc
     1261 aggcgataat cgatgtgatg gcaccggcat ggtgccgtcc attattatcg cggatgccgg
     1321 aagttaacga agctattcct atgcctctcg gtcacggagc gctggaaatc ggcgaacgcc
     1381 gcaaactggg tcatagtctg cgtgaaaagc gctacgaccg cgcctacgtc ttacctaact
     1441 ccttcaaatc cgcattagtg cctttcttcg cgggtattcc tcatcgcacc ggctggcgcg
     1501 gcgagatgcg ctacggttta ctcaacgatg tacgcgtgct cgataaagaa gcctggccgc
     1561 taatggtgga acgctatgtc gcgctggcct atgacaaagg cattatgcgc acagcacaag
     1621 atctgccgca gccattgtta tggccgcagt tgcaggtgag cgaaggtgaa aaatcatata
     1681 cctgtaatca attttcgctt tcatcagaac gtccgatgat tggtttttgc ccgggtgcgg
     1741 agtttggtcc ggcaaaacgc tggccacact accactatgc ggagctggca aagcagctga
     1801 ttgatgaagg ttatcaggtg gttctgtttg gctctgcgaa agatcatgaa gcaggcaatg
     1861 agattcttgc cgctttgaat accgagcagc aggcatggtg tcggaacctg gcgggggaaa
     1921 cacagcttga tcaagcggtt atcctgattg cagcctgtaa agccattgtc actaacgatt
     1981 ctggcctgat gcacgttgcg gcggcgctca atcgtccgct ggttgccctg tatggtccga
     2041 gtagcccgga cttcacaccg ccgctatccc ataaagcgcg cgtgatccgc ctgattaccg
     2101 gctatcacaa agtgcgtaaa ggtgacgctg cggagggtta tcaccagagc ttgatcgaca
     2161 ttactcccca gcgcgtactg aaagaactca acgcgctatt gttacaagag gaagcctgac
     2221 ggatgcgggt tttgatcgtt aaaacatcgt cgatgggcga tgttctccat acgttgcccg
     2281 cactcactga tgcccagcag gcaatcccag aaattaagtt tgactgggtg gtggaagaag
     2341 ggttcgcaca gattccttcc tggcacgctg ccgttgagcg agttattcct gtggcaatac
     2401 gtcgctggcg taaagcctgg ttctcggccc ctataaaagc tgaacgcaaa gcgtttcgtg
     2461 aagcgctaca agcagagaac tatgacgcag ttatcgacgc tcaggggctg gtaaaaagcg
     2521 cggcgctggt tacgcgtctg gcacatggcg taaagcatgg catggactgg caaaccgctc
     2581 gcgaaccttt agccagcctg ttttacaatc gtaagcatca tattgcaaaa cagcagcacg
     2641 ccgtagaacg cacccgcgaa ctgtttgcca aaagtttggg ctatagcaaa ccgcaaaccc
     2701 agggcgatta tgctatcgca cagcattttc tgacgaacct gcctacagat gctggcgaat
     2761 atgccgtatt tcttcatgcg acgacccgtg atgataaaca ctggccggaa gaacactggc
     2821 gagaattgat tggtttactg gctgattcag gaatacggat taaacttccg tggggcgcgc
     2881 cgcatgagga agaacgggcg aaacgactgg cggaaggatt tgcttatgtt gaagtattgc
     2941 cgaagatgag tctggaaggc gttgcccgcg tgctggccgg ggctaaattt gtagtgtcgg
     3001 tggatacggg gttaagccat ttaacggcgg cactggatag acccaatatc acggtttatg
     3061 gaccaaccga tccgggatta attggtgggt atggggagaa tcaagtagag tgtcgttcta
     3121 caagtatgtc tcttgcagat ttgccagctc aaaccgtttt tcaaaattta aatcttgaaa
     3181 taataaccaa taagttgaca tcggagataa gatgacctca acattatttt tctctctcga
     3241 gaaaaaaaac tggatagcgt actggaacag agctctcgta ttcttattca ttaccaccta
     3301 ttttttgggt gggattacaa ggtataaaca tc
//
LOCUS       DBFIFI010000310         3318 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.311, whole genome shotgun sequence.
ACCESSION   DBFIFI010000310 DBFIFI010000000
VERSION     DBFIFI010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.311"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..607
                     /locus_tag="N7B39_004659"
     CDS             <1..607
                     /locus_tag="N7B39_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HGU2424484.1"
                     /translation="LRALIPQRLIAFDDAVRRTLKEEEKLVNSSDWGYDTQAFARWRP
                     EYGYFAKQAGFTVKTSASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAK
                     GRPEREYLQTGDAVDSWKVIVVEPEKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVR
                     AFWHPHGMPGLFYWLLMIPAHLFIFRGMAKRIARLAEQSTD"
     gene            706..1719
                     /gene="ybjS"
                     /locus_tag="N7B39_004660"
     CDS             706..1719
                     /gene="ybjS"
                     /locus_tag="N7B39_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HGU2424485.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(1716..2546)
                     /gene="amiD"
                     /locus_tag="N7B39_004661"
     CDS             complement(1716..2546)
                     /gene="amiD"
                     /locus_tag="N7B39_004661"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HGU2424486.1"
                     /translation="MRRVFWLIAVALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(2543..2866)
                     /gene="ybjQ"
                     /locus_tag="N7B39_004662"
     CDS             complement(2543..2866)
                     /gene="ybjQ"
                     /locus_tag="N7B39_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HGU2424487.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            complement(3077..>3318)
                     /locus_tag="N7B39_004663"
     CDS             complement(3077..>3318)
                     /locus_tag="N7B39_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HGU2424488.1"
                     /translation="LYSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSC
                     FAAADQKAIQRYIPQAQDGEDFRNHLQYKLTLPTH"
BASE COUNT          786 a          888 c          837 g          807 t
ORIGIN      
        1 gctacgtgcg ctcatcccac aacggctgat cgctttcgat gacgcggtac gtcgcaccct
       61 gaaagaagaa gaaaagctgg tcaactccag cgactgggga tacgacactc aggcctttgc
      121 ccgctggcga ccagagtatg gttattttgc caaacaggcg ggatttaccg ttaaaacgtc
      181 cgccagcctt gcggctttat ggcaggtggt gaaccaaatc ggcggtaaag agcgttattt
      241 ctttggcaat attttgtggc agacacgggc gttgatggac cgtgcgatcg gtcataaatt
      301 agcgaaaggc cgtccggagc gcgaatattt gcaaactggc gatgcggtgg atagctggaa
      361 agtgattgtc gttgaaccgg aaaaacaact tacgttgtta tttggcatga aagcaccggg
      421 gctgggacga ctgtgtttta ccctggaaga taaaggcgac tatcgtacta tcgatgtccg
      481 cgctttctgg catccgcacg gtatgccggg gctgttttac tggttattga tgatccccgc
      541 gcatctgttt atttttcgcg gaatggcaaa acgaatcgcc agactggcag aacaaagcac
      601 agattaatcg acaaaacctg cgttattctt tcatgtttcc cattgcatca agggggaaat
      661 cacggaagaa tgcgcacgtc attttctcaa caacagtgga ttgatatgaa ggtactggtt
      721 accggcgcca ccagcggctt aggtcgaaac gcggtagagt ttttatgcca gaaaggcatc
      781 agcgtgcgag cgaccggtcg caacgaggca atgggtaaat tgctggaaaa aatgggcgca
      841 gagtttgttc cggcggatct gaccgagctg gtctcatcac aagctaaagt gatgctcgcg
      901 ggcattgata cgctgtggca ctgctccagc tttacctctc cctgggggac acaacaggct
      961 ttcgatctgg ctaacgttcg cgccactcgc cgcctaggtg aatgggctgt cgcctggggt
     1021 gtacgtaact ttattcatat ctcttctccc tccctgtact tcgattatca ccaccatcgc
     1081 gatattaaag aagattttcg ccctcaccgc ttcgccaacg agtttgcccg cagcaaagcg
     1141 gccagcgaag aagtgatcaa tatgctttcg caggcgaatc cacaaacgcg ctttactatt
     1201 ctgcgcccac aaagtctgtt cggaccgcac gataaagtct ttatcccccg tcttgcgcat
     1261 atgatgcacc actacggcag cattctgtta ccgcatggcg gcagtgcgct ggtggatatg
     1321 acctactatg aaaatgccgt gcacgccatg tggctggcaa gccaggaagc ctgcgataag
     1381 ctaccttccg ggcgtgtgta caacatcacc aacggcgagc atcgcacact gcgcagcatc
     1441 gtgcagaagc tgatcgacga gttgaatatt gactgtcgta ttcgttccgt cccttacccg
     1501 atgctggata tgatcgcccg cagcatggag cgtttaggcc gcaagtcagc aaaagagccg
     1561 ccgctgaccc actacggcgt ctcgaagctt aattttgact ttacgctgga tattacgcgg
     1621 gcacaggaag agttaggtta tcagccggtc atcaccctgg atgaaggtat cgagaaaact
     1681 gccgcctggc tgcgcgacca cggaaaactg ccgcgctaat cctgcccgta tttctccagc
     1741 aacgcttcgg caatcgcctg agtttctgca tccgcttcgc cgttatataa cgtcgggcgg
     1801 aaatgcatct ggaacgccat aatcacgcgc cgctgctcgc gcggtgtcat atcaggttta
     1861 acgtcataac cgtagcgcgc caaaagctcc agcaatgacg cggtatctac cggtgtgtgc
     1921 ggcgcgcgcc cggcaaggta aaagttcacc cgctgcgcat ccggccaggc accaatcccc
     1981 tgctgcgcca gttgctgcca gggaaataat ggccccggat cgtctttgcg ctgcggggcg
     2041 atatccgcat gtgccactac gttttccggc ttgatgtgat aacgggcaat aatatctttc
     2101 gccagcggaa taagcgcctg aatctgtgcc ggttcaaacg gggcaaaata tttcaccccc
     2161 gccgattttt gccagccacg gttttccagc tcaatgccaa tagaggtgtc gttaaggcgc
     2221 gttgccccgc gccaggcgct aatccccgca tgccaggcca gttcttgttc cggcaccaat
     2281 tgccagatgc gcggtttacc gttgtatcgc ggtggtaccg cagggaccag ataatgcgag
     2341 ctgacctgct tatcggtcag tgtcgccagc gagctatcaa aatcatctgc ggtgtagtgg
     2401 atcaccagca ctttaatgcg cggatacgcc gcctgcgcct ggcgtcgggt atcaagctga
     2461 tatccctctt tctcgacaat gcctttttcg cctgcgcacc ctgccagtaa cagagcgaca
     2521 gcgatcagcc agaagactct tctcatcgac gcgttttcac cgccgtaccg ctgacgctaa
     2581 ccatcagcat actgccgttt tgcccgaccg tttcgtagtc gatatcaata ccgactacgg
     2641 catcggcccc cagcgcccgc gcctgggagc caagctcctc aaaggcgatc tcccgcgctt
     2701 tacgtagctc tttttcgtag gcaccggagc gtccgccaac gatatcgcga ataccagcaa
     2761 agaaatcacg gaaaatattc gcgcctaaaa tcgcttcgcc agtcaccaca ccgcaatatt
     2821 caacgatggt ctggccttcc agagttggag ttgtggaaaa ttgcatgatc gatctccttg
     2881 tttgttaagc cttcgatctc taaagcatta tcggactgat atgccatgtt gggaagaagg
     2941 ttttgatcag tttaggaaat catctcattg tgtcaaaaac agctacataa gcctgtacaa
     3001 ttatctgttt cgtaaccgga tattggtttc attatggagc accgcttacc tgccctatta
     3061 tgttgtgccg gcaacattaa tgagtgggaa gagtcaactt atattgtaaa tgattcctaa
     3121 agtcttcccc atcttgcgcc tgaggtatat accgctggat tgctttttgg tccgccgccg
     3181 caaaacaaga aagtaataag atctcaatgc ttttgtcatt aatataagcg aagatcattc
     3241 ttgcaaattc gtatataaac gcccgatcct gagcgcttaa tagtttaata caatatttat
     3301 ttttaagaga ataaagta
//
LOCUS       DBFIFI010000311         3289 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.312, whole genome shotgun sequence.
ACCESSION   DBFIFI010000311 DBFIFI010000000
VERSION     DBFIFI010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.312"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            321..1637
                     /gene="shiA"
                     /locus_tag="N7B39_004664"
     CDS             321..1637
                     /gene="shiA"
                     /locus_tag="N7B39_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HGU2424489.1"
                     /translation="MDSTLISTRPDERTVSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQYNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDNQRA"
     gene            1739..3193
                     /gene="amn"
                     /locus_tag="N7B39_004665"
     CDS             1739..3193
                     /gene="amn"
                     /locus_tag="N7B39_004665"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HGU2424490.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT          759 a          806 c          796 g          928 t
ORIGIN      
        1 aaaacataac taacctcgcc tccctactgg ttataatgca atgcagtcta tcagaatcat
       61 cagggtgcaa ttttgtgcat atgcggactt ttatgtttca tatctcgaac ctgtggggcc
      121 tctgcttaat ccttaaacaa caccagcaac tcctgcgctt tcatcttcca gcgaattttt
      181 catgttgccg ttaatcagcc ataaaatcat ttgcagatgc gctctgtcga ggtagtctca
      241 taaggttcgt ttatagatcg acgcaatgtg agttaccttt tccatactaa ttataaaaag
      301 acagtacaaa caggatcatt atggactcca cgctcatctc cactcgtccc gatgaaagga
      361 cggtttcgtt aagtcgcgcc cgacgagctg cgttaggcag tttcgctggt gccgtcgtcg
      421 actggtatga ttttttactc tatggcatta ccgccgcact ggtgtttaat cgcgagtttt
      481 tcccgcaagt aagcccggct atgggaacgc tcgccgcatt tgccaccttt ggcgtcggat
      541 tccttttccg tccgctcggc ggtgtcattt tcggtcactt tggcgaccga ctgggacgta
      601 agcgcatgtt aatgctgacc gtctggatga tgggcatcgc gacagccttg attggtattc
      661 ttccttcatt ctcgaccatt gggtggtggg cacctatttt actggtgacg ctgcgtgcca
      721 ttcagggatt tgccgtcggc ggcgaatggg gaggcgcggc gttgctttcc gttgaaagtg
      781 caccgaaaaa taaaaaagcc ttttacagta gcggtgtaca agttggctac ggtgtaggtt
      841 tactgctttc aaccggactg gtttcattga tcagtatgat gacgactgac gaacagtttt
      901 taagctgggg ctggcgcatt cctttcctgt ttagcatcgt actggtactg ggagcattgt
      961 gggtgcgcaa tggcatggaa gagtccgcgg aatttgaaca acagcaatat aatcaagcgg
     1021 ccgcgaaaaa acgcatcccg gttatcgaag cgctgttacg acatcccggt gctttcctga
     1081 agattattgc gctacgactg tgcgagttgc tgacaatgta catcgttacc gcctttgcac
     1141 ttaattattc aactcagaat atggggttac cgcgcgaact tttccttaat attggtttgc
     1201 tggtaggtgg attaagctgc ctgacaattc cctgttttgc ctggcttgcc gatcgttttg
     1261 gtcgccgcag ggtttatatc acaggcgcgt tgatcggaac gttgagcgca tttcctttct
     1321 ttatggcgct tgaagcacaa tctattttct ggatagtttt cttctccata atgctggcaa
     1381 acattgcgca tgacatggtg gtgtgtgtgc aacaaccgat gtttaccgaa atgtttggtg
     1441 ccagttatcg ctatagtggt gctggagtcg gttatcaggt tgccagtgtg gttggcggtg
     1501 gatttacacc ttttattgcc gctgcgctca tcacttactt tgccgggaac tggcatagcg
     1561 tcgccattta tttgctggct ggatgtctga tttccgcaat gaccgctttg ttgatgaaag
     1621 acaatcaacg cgcttgatag tctggcgaag atcatccgat cttcgcctta cacttttgtt
     1681 tcacattcct gtgacatact atcggatgtg cggtaattgt atggaacagg agacacacat
     1741 gaataataag ggctccggtc tgaccccagc tcaggcactg gataaactcg acgcgctgta
     1801 tgagcaatct gtagtcgcat tacgcaacgc cattggcaac tatattacaa gtggcgaatt
     1861 acctgatgaa aacgcccgca aacaaggtct ttttgtctat ccatctctga ccgtaacctg
     1921 ggacggtagc acaacaaatc cccccaaaac gcgcgcattt ggtcgtttta ctcacgcagg
     1981 cagctacacc accacgatta ctcgccctac tctctttcgt tcgtatctta atgaacaact
     2041 tacgttgctg tatcaggatt atggcgcgca tatctcggtg caaccctcac agcatgaaat
     2101 cccttatcct tatgtcatcg atggctctga attaacactt gatcgctcaa tgagcgctgg
     2161 gttaactcgc tatttcccga caacagaact ggcgcaaatt ggcgatgaaa ctgcagacgg
     2221 catttatcat ccaactgaat tctccccgct atcgcatttt gatgcgcgcc gcgtcgattt
     2281 ttccctcgcg cggttgcgcc attataccgg tacgccagtt gaacattttc agccgttcgt
     2341 cttgtttacc aactacacac gttatgtgga tgaattcgtt cgttggggat gcagccagat
     2401 cctcgatcct gatagtccct acattgccct ttcttgtgct ggcgggaact ggatcaccgc
     2461 cgaaaccgaa gcgccagaag aagccatttc cgaccttgca tggaaaaaac accagatgcc
     2521 agcatggcat ttaattaccg ccgatggtca gggtattact ctggtgaata ttggcgtggg
     2581 accgtcaaat gctaaaacca tctgcgatca tctggcagtg ctacgcccgg atgtctggtt
     2641 gatgattggt cactgtggcg gattacgtga aagtcaggcc attggcgatt atgtacttgc
     2701 acacgcttat ttacgcgatg accacgttct tgatgcggtt ctgccacccg atattcctat
     2761 tccgagcatt gctgaagtgc aacgtgcgct ttatgacgcc accaagctgg tgagcggcag
     2821 gcccggtgag gaagtcaaac agcggctacg tactggtact gtggtaacca cagatgacag
     2881 gaactgggaa ttacgttact cagcttctgc acttcgtttt aacttaagcc gggccgtagc
     2941 aattgatatg gaaagtgcaa ccattgccgc gcaaggatat cgtttccgcg tgccatacgg
     3001 gacactactg tgtgtttcag ataaaccgtt gcatggcgag attaaacttc ccggtcaggc
     3061 taaccgtttt tatgaaggcg ctatttccga acacctacaa attggcattc gggcgatcga
     3121 tttgctgcgc gcagaaggcg accgactgca ttcacgtaaa ttacgaacct ttaatgagcc
     3181 gccgttccga taataagttc tgaaccttct tatcaaaaat agatgtgaag gattaagtaa
     3241 gacatctggc aaatagcccg caataaacgc gggctgtttg cttccttgg
//
LOCUS       DBFIFI010000312         3287 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.313, whole genome shotgun sequence.
ACCESSION   DBFIFI010000312 DBFIFI010000000
VERSION     DBFIFI010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.313"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..799)
                     /locus_tag="N7B39_004666"
     CDS             complement(<1..799)
                     /locus_tag="N7B39_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HGU2424491.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFFSCLVFVFFYFAILMTVSADMPLIGCV
                     LTLSLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGVWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIVSVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLPFLAVAGYIIFDQILNAYDSSVYANDDI"
     gene            992..1495
                     /gene="yjgM"
                     /locus_tag="N7B39_004667"
     CDS             992..1495
                     /gene="yjgM"
                     /locus_tag="N7B39_004667"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU2424492.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLAGSESDICELQKMYFLPAIRGK
                     GLAKKLALKAMEEAREMGFKRCYLETTAFLKEAIGLYEHLGFQHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(1541..1957)
                     /gene="rraB"
                     /locus_tag="N7B39_004668"
     CDS             complement(1541..1957)
                     /gene="rraB"
                     /locus_tag="N7B39_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HGU2424493.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            2119..3123
                     /gene="argF"
                     /locus_tag="N7B39_004669"
     CDS             2119..3123
                     /gene="argF"
                     /locus_tag="N7B39_004669"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HGU2424494.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKPFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            complement(3180..>3287)
                     /locus_tag="N7B39_004670"
     CDS             complement(3180..>3287)
                     /locus_tag="N7B39_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424495.1"
                     /translation="VSPFIDQQRLNTLNPPENLRIAIEKFGWKKKTITA"
BASE COUNT          980 a          747 c          716 g          844 t
ORIGIN      
        1 caatatcatc atttgcatac acagaactat catacgcatt taatatttga tcgaagataa
       61 tataaccagc aacagcgaga aaaggtaata aagctaaaaa tgcgaggatg gcatatttta
      121 tacaatacgc agtatctaat ttcaccttga aacgatgtat accgaaactg gtattgctcc
      181 agagaaaact cattaccagg ctatataaag taccattaaa aataccaatg gaaacaatac
      241 caactattgc catcagaacc acagatacaa taacactact tgaactattg gcatgtagca
      301 tctttgtcga gataaagaaa acagtcccca tcccaatggc cattaaaatc ggcaagaaaa
      361 aggttaccca ccagacccct ttcatagagc aattaaaact aaatcttacg ccgttgagac
      421 tggtcatcaa ggcctgataa cgcagtcctt ttgctgccat aaatataagc aaaaccaaca
      481 gtgacaaagt caaaacacag ccaataagcg gcatatctgc tgacactgtc attaagattg
      541 cgaaatagaa aaaaacaaaa acaagacaac taaaaaaaac attcccacca gtaattccat
      601 aagaaaatcg ttgtccgtta acttccatat tagcatagag ataacgctta catttcatta
      661 atgcccatgg taaatagata cctagcgtta taatcgttaa caacacattc accacacaaa
      721 taagaaaata tctctcacct gtaccatgaa aaacaaacga atgggacgga acatccattt
      781 cattaataac ttgagccata aaaattccct gaattaaaaa atccattgat ataatttata
      841 gatacagaca gtaaaatgtt ctgccacgtt acaatactct tatcagtaat aggctggcaa
      901 aaactttatt gctttataag acgcttgcgc acgtaagaag agagtggtat taaacaaatg
      961 tataattact ttatttttat tgggttaaac tatgaataac attgcgccgc aatcacctgt
     1021 aatgcgtcgc ctgacgctgc aggataatcc tgctatcgcc cgcgtcattc gtcaggtatc
     1081 cgccgaatac ggtcttaccg ctgataaagg ctacaccgtc gccgatccga atcttgacga
     1141 gctgtatcaa gtgtatagtc agccgggtca tgcatattgg gtggttgagt acgagggtga
     1201 agtggtcggc ggcggtggga ttgcgccatt agcaggtagt gaatcggaca tttgcgaact
     1261 gcaaaagatg tattttcttc cggctattcg cggcaaaggg ctggcaaaaa aactggcctt
     1321 aaaggcgatg gaggaggcaa gagaaatggg cttcaaacgc tgctatctgg aaacgaccgc
     1381 ttttttgaaa gaagcgattg gtttgtatga acatttaggt tttcaacata tcgactatgc
     1441 acttggctgc acgggccatg ttgattgtga agtgcggatg ctacgcgaac tctaaaaaaa
     1501 tgcccgctag tgtgtgctat cgggcatttt aatcaattaa ttagtggcga accccgtcat
     1561 cgtcttcatc gacaaaatct tcatcgtcgc cgtcttcgcc gttgggatct tcaaagtaag
     1621 tgccccaacc gtcgtactca acgtcaaatt tctccgccag cgtcatcagt tgttcaacct
     1681 gggcatcgat cagatcggca ttcaacgcgc actcgctgag gatgtcgcag caaatcacga
     1741 tatcaccatc ttcaacttcc agctcttctg gatcggtcac ttcgtaaccg agtttaaacg
     1801 cttcaactgc tgctttttcc agggtttcca gatcgtctgc ggaaagatgg tgttcgatgg
     1861 tgtacagcgc gtccggatcg ctgccatctt ccagtaattc ttcaataatc aaacgtgttt
     1921 cttcacgctg ttcttccagt tgttccgggt ttgccatggc tcattcctct taatgtcctg
     1981 ccgatactca tattgtcaca caccactggc attgcctcca cctttgtgac aaagatttat
     2041 gctttagact tgcaaatgaa taatcatcca tataaattga attttaattc attgagacgt
     2101 tagccacagg agggatctat gtccgggttt tatcataagc atttcctgaa attactcgat
     2161 ttcacgccag ctgaactcaa cagcctgctg cagttagccg cgaagctgaa agccgataag
     2221 aaaagcggca aagaagaagc caaactcact ggtaaaaaca tcgcgctcat cttcgaaaaa
     2281 gactcgaccc gtacccgatg ctctttcgaa gttgccgcat atgaccaggg tgctcgcgtt
     2341 acttatctcg gcccaagcgg cagtcagatt ggtcataaag agtcgattaa agacactgcc
     2401 cgcgtgctgg gtcgcatgta tgacggtatt cagtatcgcg gctatggtca ggagattgtc
     2461 gaaacactgg cggaatacgc tggcgtgccg gtatggaacg gcctgaccaa tgagtttcac
     2521 cccacgcagt tgctggcgga tctcctcacc atgcaggagc atttacccgg taaaccattc
     2581 aacgaaatga cgctggtcta tgcaggtgac gcacgtaaca acatgggcaa ttcgatgctc
     2641 gaagctgcgg cgcttaccgg tctggatttg cgtctggtcg cgccacaggc atgctggccg
     2701 gaagctgcgc tggttacgga atgccgcgcc ctggcacaac aaaatggcgg gaatattacg
     2761 ctgacggaag atgtagctaa aggcgttgaa ggtgctgact ttatctatac cgatgtatgg
     2821 gtgtcgatgg gtgaagcaaa agaaaaatgg gctgaacgca ttgcattact gcgtgattat
     2881 caggtgaaca gcaagatgat gcagttgacc ggtaacccgg aggtcaaatt cctccactgc
     2941 ctgcccgcgt tccatgatga ccaaacgacg cttggcaaga aaatggcgga agagtttggc
     3001 ctacatggcg gaatggaagt gactgatgag gtcttcgaat ctgccgccag cattgttttt
     3061 gatcaggcgg aaaaccgcat gcacaccatc aaagcggtga tggtcgcgac actcagtaaa
     3121 taattgatat cgctataaag atgccgggaa atacagaaac atcccggcgt caaattattt
     3181 tatgcagtga tagttttttt cttccagcca aatttttcta ttgctatacg taaattttcc
     3241 ggaggattta gtgtatttaa acgctgttgg tcaataaatg gcgatac
//
LOCUS       DBFIFI010000313         3287 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.314, whole genome shotgun sequence.
ACCESSION   DBFIFI010000313 DBFIFI010000000
VERSION     DBFIFI010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.314"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..528)
                     /locus_tag="N7B39_004671"
     CDS             complement(<1..528)
                     /locus_tag="N7B39_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HGU2424496.1"
                     /translation="MAKPARRRCKNDECREWFHPAFANQWWCSPECGTKIALERRSKE
                     REKAEKAEKAAEKKRRREEQKQKDKLKIQKLALKPRSYWIKQAQQAVHAFIRERDRDL
                     PCISCGTLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELIS
                     RIGQEAVEEIESNHNR"
     gene            complement(521..691)
                     /locus_tag="N7B39_004672"
     CDS             complement(521..691)
                     /locus_tag="N7B39_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinF"
                     /protein_id="HGU2424497.1"
                     /translation="MIDQNRSYEQESVERALTCANCGQKLHVLEVHVCEHCCAELMSD
                     SNSSMHEEKDDG"
     gene            complement(688..870)
                     /locus_tag="N7B39_004673"
     CDS             complement(688..870)
                     /locus_tag="N7B39_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HGU2424498.1"
                     /translation="MRRQRRSFTDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHCARKTR"
     gene            900..1571
                     /gene="bet"
                     /locus_tag="N7B39_004674"
                     /pseudo
     CDS             900..1571
                     /gene="bet"
                     /locus_tag="N7B39_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
     gene            1568..2248
                     /locus_tag="N7B39_004675"
     CDS             1568..2248
                     /locus_tag="N7B39_004675"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HGU2424499.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVVERDEKYMAGFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            2245..2427
                     /locus_tag="N7B39_004676"
     CDS             2245..2427
                     /locus_tag="N7B39_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HGU2424500.1"
                     /translation="MKHPHDNIRVGTITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEE
                     INNKREAICTKHLLLS"
     gene            2400..2591
                     /locus_tag="N7B39_004677"
     CDS             2400..2591
                     /locus_tag="N7B39_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HGU2424501.1"
                     /translation="MHKASPVELRTSIEMAHSLAQIGVRFVPIPVETDEEFHTLATSL
                     SQKLEMMVAKAEADERDLV"
     gene            2668..2883
                     /locus_tag="N7B39_004678"
     CDS             2668..2883
                     /locus_tag="N7B39_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HGU2424502.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQPFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            2982..3203
                     /locus_tag="N7B39_004679"
     CDS             2982..3203
                     /locus_tag="N7B39_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HGU2424503.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNHQAISATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEDLELISKQRGSK"
BASE COUNT          819 a          796 c          842 g          830 t
ORIGIN      
        1 gcggttatgg tttgattcga tttcctctac tgcttcctgc ccgatgcggc taatcagttc
       61 gacgcgatac ggaacgagat ttccgctttt gtgctggttg cacaccacgc attgcttgtg
      121 aatattgcgt tcatcaaatc ggagttgagg tgccgcagca gttgtccggt aatgtccggc
      181 atcccactga gcagacgtga gcgttccgca cgagatacat ggtaagtcgc ggtctctttc
      241 tctgatgaag gcgtgtacgg cttgttgggc ttgtttaatc cagtaactgc ggggctttaa
      301 ggcgagtttt tgaatcttaa gtttatcttt ctgtttctgc tcctctcgtc gtcgtttctt
      361 ctctgctgcc ttttctgcct tttccgcttt ttcgcgttct ttgcttcgtc gttcgagtgc
      421 tatcttggtt ccacactctg gagagcacca ccactgatta gcgaatgcag ggtgaaacca
      481 ttcccgacat tcatcgtttt tacatcgtct tcgcgctggt ttagccatca tctttttcct
      541 cgtgcatcga gctattcgaa tcgctcatca gctctgcgca gcagtgctca cacacgtgaa
      601 cttccagcac atgcagcttc tgaccgcagt tagcgcacgt taaagcccgc tcgacgcttt
      661 cttgttcgta acttcgattt tggtcaatca ccttgttttc ctcgcacaat gtcttagcca
      721 ccggatatcc cacaggtgag ccgtgtagtt gaaggttttt acgtcagatt cttttgggat
      781 tggcttgcgt ttatttctgg agcgtttcgt tggaaggtat ttgcagtttt cgcagatgat
      841 gtcggtgaaa cttcgtcgct gtcgtctcat gccgccctgt ctccccatcg cgctttcatg
      901 ccagcgatgc gcagttcatc gcattgttga tcgtcgccaa ccagtacggc cttaatccgt
      961 ggacgaaaga aatttacgcc ttccctgata agcagaacgg catcgttccg gtggtgggcg
     1021 ttgatggctg gtcccgcatc atcaatgaaa accagcagtt tgatggcatg gactttgagc
     1081 aggacaatga atcctgtaca tgccggattt accgcaagga ccgtaatcat ccgatctgcg
     1141 ttaccgaatg gatggatgaa tgccgccgcg aaccattcaa aacccgcgaa ggcagagaaa
     1201 tcacggggcc gtggcagtcg catcccaaac ggatgttacg gcataaagct atgattcagt
     1261 gtgcccgtct ggccttcgga tttgctggta tctatgacaa ggatgaagcc gagcgcattg
     1321 tcgaaaatac tgcatacact gcagaacgcc agccggaacg cgacatcact ccggttaacg
     1381 atgaaaccat gcaggagatt aacactctgc taatcgccct ggataaaaca tgggatgacg
     1441 acttattgcc gctctgttcc cagatatttc gccgcgacat tcgcgcatcg tcagaactga
     1501 cacaggccga agcagtgaaa gctcttggat tcctgaaaca gaaagccact gagcagaagg
     1561 tggcagcatg acaccggaca ttatcctgca gcgtaccggg atcgacgtga gagctgtcga
     1621 acagggggat gatgcatggc acaaattacg gctcggcgtc atcaccgctt cagaagttca
     1681 caacgtgata gcaaagcccc gctcaggaaa gaagtggcct gacatgaaaa tgtcctactt
     1741 ccacaccctg ctggctgagg tttgcaccgg tgtggctccg gaagttaatg ctaaggcgct
     1801 ggcctgggga aaacagtacg agaacgacgc cagaaccctg tttgaattca cttccggcgt
     1861 gaatgttact gaatccccga tcatctatcg cgacgaaagt atgcgcaccg cctgctctcc
     1921 cgatggttta tgcagtgacg gcaacggcct tgagctgaaa tgcccgttta cctcccggga
     1981 tttcatgaag ttcaggctcg gtggtttcga ggccataaag tcggcttaca tggcccaggt
     2041 gcagtacagc atgtgggtga cgcgaaaaga tgcctggtac tttgccaact atgacccgcg
     2101 tatgaagcgt gaaggcctgc attatgtcgt ggttgagcgg gatgaaaagt acatggcggg
     2161 ttttgacgag atggtgccgg agttcatcga aaaaatggac gaggcactgg ctgaaattgg
     2221 ttttgtattt ggggagcaat ggcgatgaag catcctcacg ataatatccg cgtaggcacg
     2281 atcactttcg tctactccgt tacgaagcga ggctgggtat ttcccggcct ttctgttatc
     2341 cgaaatccgc tgaaagccca gcggctggct gaggagataa ataataaacg ggaggcgata
     2401 tgcacaaagc atctcctgtt gagttaagaa cgagtattga gatggcacat agccttgctc
     2461 aaattggagt caggtttgtg ccaataccag tagaaacaga cgaagaattt catacgttag
     2521 ccacatccct ttcgcaaaag ctggaaatga tggtggcgaa agcagaagca gatgagagag
     2581 acctggtatg acaaccactg aatgcatttt tctggcagcg ggcttcatat tctgtgtgct
     2641 tatgcttgcc gacatgggac ttgttcaatg acacctcagc aagaaaacgc ccttcgcagt
     2701 attgcccgtc aggctaattc tgaaatcaaa aaagccagac agccgtttcc ggataaaaac
     2761 gtcgatgaca tttgccgtag cgtactgaag aagcaccgcg aaacggtaac gctgatggga
     2821 ttcacaccga ctcacttaag tctggcaatc ggcatgttaa acggcgtctt taaggaacgg
     2881 tgaacatgaa aagcaaaatc atcagggagc tacaggctcc ttttttatta ttcgcattca
     2941 ccctcaagcg tattaaccaa caattcaggg attaatggaa gatggcagac atcattgatt
     3001 cagcatcaga aattgaagaa ttacagcgca acacagcaat aaaaatgcgc cgcctgaacc
     3061 accaggctat atctgccact cattgttgtg agtgtggcga tcccatagat gagcgaagac
     3121 gcctggccgt tcagggttgt cggacttgtg caagttgcca ggaggatctg gaacttatca
     3181 gtaaacagag aggttcgaag tgagcgaaat taactagaag ccaaagataa aatcatcgct
     3241 gagcaggaga aaatcgctaa cggagaaaag acagtaagtc agtatat
//
LOCUS       DBFIFI010000314         3252 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.316, whole genome shotgun sequence.
ACCESSION   DBFIFI010000314 DBFIFI010000000
VERSION     DBFIFI010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3252
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.316"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..147
                     /locus_tag="N7B39_004680"
     CDS             <1..147
                     /locus_tag="N7B39_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU2424504.1"
                     /translation="LSWHGGYRAFLWRPPKHNRTVRVVCREWSVTDNARYSDFSCTIE
                     QVVN"
     gene            147..845
                     /locus_tag="N7B39_004681"
     CDS             147..845
                     /locus_tag="N7B39_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HGU2424505.1"
                     /translation="MQNIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KREPVTWQGRQYQAYPIEGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELRDRWVVEQMSELTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWTYRSDECGYTGGAVADEFDNPTTDIRKDRCSKCMRGCEMRSMV
                     ANFGGFLSINKLSQ"
     gene            856..1599
                     /locus_tag="N7B39_004682"
     CDS             856..1599
                     /locus_tag="N7B39_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HGU2424506.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVISTPEGEWYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGDIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGTDMPDFEREDDWWRNGQNLYLDNMEAT
                     GFYRISLPSAQPGDILLCCFGASVANHAAIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSAWRHRHWHVSAFTGIYNDLAAASACM"
     gene            1596..2177
                     /locus_tag="N7B39_004683"
     CDS             1596..2177
                     /locus_tag="N7B39_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HGU2424507.1"
                     /translation="MNTAAEAIRALSLQVPGFRRQMNEGWYQIRIAGYDTAPEAVYAR
                     LHEQLGEGTVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGASMALWGSALAAGGFS
                     ATTMLFSLGASMILGGVAQMLAPKAKTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGGGTVVVVGRQG"
     gene            2519..>3252
                     /locus_tag="N7B39_004684"
     CDS             2519..>3252
                     /locus_tag="N7B39_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU2424508.1"
                     /translation="MGKGGGKAHTPREAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETGLGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLLETTSKGDRNHSSVRLLIQLQRNGNWVTEKDVTINGKTT
                     SQFLASVILDNLPERPFNIRMVRETADSTSDQLQNKTLWSSYTEIIDVKQCYPNTAIV
                     GLQVDAEQFGGQQMTVNYHIRGRIIQV"
BASE COUNT          721 a          780 c         1051 g          700 t
ORIGIN      
        1 ttatcctggc atggtggtta ccgtgccttt ttgtggcgac cgccgaaaca taaccggacg
       61 gtgagggttg tgtgccgtga gtggagtgtc acggataatg cccggtacag tgatttcagt
      121 tgcacgatag agcaggtggt gaactgatgc agaatattca tgaagaaagc ctgaacgagt
      181 cggttaagtc agagcagtca ccgcgggtgg tgctctggga aatcgacctg acggtgcagg
      241 gcggtgagcg gtattttttc tgcaatgagc tgaatgaaaa aagggagccg gtgacctggc
      301 aggggcggca atatcaggcg tacccgattg agggcagtgg ctttgagatg aacggaaagg
      361 gcagtagtgc ccgcccgtcg ctgacggtgt ccaatctgtt cggtcttgtc accgggatgg
      421 cggaggattt gcagagcctg gtgggtgcca cggtggtccg ccgccgggtg tatgcccgtt
      481 ttctggatgc ggtgaatttt gtggcgggca atccggaagc ggacccggag caggagctga
      541 gagaccgctg ggtggtggag cagatgtcag agctgacggc catgacagcc tcgtttgtgc
      601 tggcaacacc gacggagacg gacggagcgc tgtttcccgg tcgcattatg ctggcgaata
      661 cctgtatgtg gacctaccgc tctgatgagt gtggttacac gggcggggca gtggcggatg
      721 agttcgacaa ccccaccacg gatatccgta aggacagatg cagcaagtgc atgcgcgggt
      781 gtgagatgcg cagcatggtg gctaattttg gcggtttcct ttccattaat aaactttcgc
      841 agtaaatccc ggtttatgac acagactgaa tcagcgattc tggcgcatgc ccggcggtgt
      901 gcgcctgcgg agtcgtgcgg cttcgtgata agcacgccgg agggggagtg gtatatccct
      961 tgtgtgaata tttctgcaga gccggaggcg tattttcgta tcgcaccgga agactggctg
     1021 cgggcagaga tgcaggggga gattgtggca ctggtccaca gtcatcccgg tgggctgccc
     1081 tggctgagcg aggctgaccg gcggctgcag ataaaaagcg cactgccctg gtggctggtc
     1141 tgccggggtg acattcacaa attccgctgt gtgccacatc tgacaggacg gcgctttgag
     1201 cacggggtga cggactgtta cacgctgttc cgggatgctt atcatctggc ggggactgat
     1261 atgccggatt ttgagcgtga ggatgactgg tggcgcaatg gtcagaacct gtacctggac
     1321 aatatggagg cgactggttt ttacaggatt tccctgcctt ccgcacagcc tggcgatatc
     1381 ctgctgtgct gctttggcgc atcggtggcc aatcatgccg ccatatactg cggcaacggt
     1441 gaactgcttc accatctgcc tgaacaactg agtaaacggg agaggtattc cgaaaaatgg
     1501 caacgacgaa cgcattcagc ctggcgtcac cgccactggc acgtatctgc cttcacgggg
     1561 atttacaacg atttggccgc cgcctcagcc tgtatgtgaa cacggcagcg gaagccatcc
     1621 gggcgctgtc gttacaggtg ccgggctttc gccgtcagat gaacgaaggc tggtaccaga
     1681 tacgtattgc cggttatgac acggcaccgg aggcggtgta cgcccgtctt cacgaacagc
     1741 tgggtgaggg aacggtcatc catattgtgc cgcgactggc cggggccgga aagggtggac
     1801 tgcagattgt gctgggggcg gcagccatcg tgggctcttt cttcactgcc ggggcatcaa
     1861 tggcgttatg gggttcagcc ctggcagccg gtggtttttc tgccaccacg atgctgtttt
     1921 cacttggagc cagcatgatt ctgggcggtg tggcccagat gctggccccg aaggcaaaaa
     1981 caccggatta ccgcgcaacg gataacggca gacagaacac gtacttttcc tcgctggata
     2041 acatgattgc ccaggggaac ccgatgccgg tgccttacgg ggaaatgctg gttggctccc
     2101 gccgtatatc ccaggacatc agcacccgtg atgaaggtgg gggcggaacg gtcgtggttg
     2161 tcgggcgaca gggataaaac ataaaaaaat cccgcagtga tcgcggagct gcggggacag
     2221 acaaatgaag atcaatgtta aggagttgtt tttgttactc gggcaaaaaa acactaacgc
     2281 agcgaaatta taagcgccac agtcagtgtg tgaaaatgtg aagatattca gaatttttat
     2341 gccattaccg gttttaacca acaggattat cggtgggcat gaaagaaaac cccggtatct
     2401 gctgataccg gggttttctt tagcatggca gaaatgtgtt tcatgctttt cgggcgaagg
     2461 atatccgact tctgtacgga atggcaagtg gcggttaatt tattcagggg aaggctgtat
     2521 gggaaaaggt ggcggtaagg cacacacgcc tcgtgaggcg aaggataatc tcaaatccac
     2581 gcagatgatg agtgtgattg atgcgattgg tgagggaccg atagaaggtc cggtgaaggg
     2641 actgcagagt attctggtga acaaaacccc actgacggac acggacggca atcccgtgat
     2701 acacggtgtg accgcggtct ggcgtgccgg ggagcaggag cagacaccac cggaaggctt
     2761 tgagtcctcc ggagctgaaa ccggactggg cgtggaagtg acgaaggcaa aaccggtgac
     2821 gcgcaccatt acgtccgcga acattgaccg cctgcgggtc accttcgggg tgcagtcact
     2881 gttggagacc acctcaaagg gcgaccgtaa tcactcttct gtccgactgc tgattcagtt
     2941 gcagcgtaac ggtaactggg tgacggaaaa ggatgtcacc attaacggca agaccacctc
     3001 gcagtttctg gcgtcggtga ttctggataa tctgcctgag cggcccttta acatccggat
     3061 ggtccgggag acagcggaca gcacctcgga ccagctgcag aataagacgc tctggtcgtc
     3121 atacaccgaa atcatcgatg tgaaacagtg ctacccgaac acggcgattg tggggctgca
     3181 ggtggatgcg gagcagtttg gcggtcagca gatgacggtg aactaccata tccgcggtcg
     3241 catcatccag gt
//
LOCUS       DBFIFI010000315         3204 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.317, whole genome shotgun sequence.
ACCESSION   DBFIFI010000315 DBFIFI010000000
VERSION     DBFIFI010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.317"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..333
                     /gene="cesT"
                     /locus_tag="N7B39_004685"
     CDS             <1..333
                     /gene="cesT"
                     /locus_tag="N7B39_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesT"
                     /protein_id="HGU2424509.1"
                     /translation="EYMMIYGVCGKFPTDNSNFALEILNANLWFAENGGPYLCYEAGA
                     QSLLLALRFPLDDATPEKLENEIEVVVKSMENLYLVLHNQGITLENEHMKIEEISSSD
                     NKHYYAGR"
     gene            393..3197
                     /gene="eae"
                     /locus_tag="N7B39_004686"
     CDS             393..3197
                     /gene="eae"
                     /locus_tag="N7B39_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin type gamma"
                     /protein_id="HGU2424510.1"
                     /translation="MITHGCYTRTRHKHKLKKTLIMLSAGLGLFFYVNQNSFANGENY
                     FKLGSDSKLLTHDSYQNRLFYTLKTGETVADLSKSQDINLSTIWSLNKHLYSSESEMM
                     KAAPGQQIILPLKKLPFEYSALPLLGSAPLVAAGGVAGHTNKLTKMSPDVTKSNMTDD
                     KALNYAAQQAASLGSQLQSRSLNGDYAKDTALGIAGNQASSQLQAWLQHYGTAEVNLQ
                     SGNNFDGSSLDFLLPFYDSEKMLAFGQVGARYIDSRFTANLGAGQRFFLPANMLGYNV
                     FIDQDFSGDNTRLGIGGEYWRDYFKSSVNGYFRMSGWHESYNKKDYDERPANGFDIRF
                     NGYLPSYPALGAKLIYEQYYGDNVALFNSDKLQSNPGAATVGVNYTPIPLVTMGIDYR
                     HGTGNENDLLYSMQFRYQFDKSWSQQIEPQYVNELRTLSGSRYDLVQRNNNIILEYKK
                     QDILSLNIPHDINGTEHSTQKIQLIVKSKYGLDRIVWDDSALRSQGGQIQHSGSQSAQ
                     DYQAILPAYVQGGSNIYKVTARAYDRNGNSSNNVQLTITVLSNGQVVDQVGVTDFTAD
                     KTSAKADNADTITYTATVKKNGVAQANVPVSFNIVSGTATLGANSAKTDANGKATVTL
                     KSSTPGQVVVSAKTAEMTSALNASAVIFFDQTKASITEIKADKTTAVANGKDAIKYTV
                     KVMKNGQPVNNQSVTFSTNFGMFNGKSQTQATTGNDGRATITLTSSSAGKATVSATVS
                     DGAEVKATEVTFFDELKIDNKVDIIGNNVRGELPNIWLQYGQFKLKASGGDGTYSWYS
                     ENTSIATVDASGKVTLNGKGSVVIKATSGDKQTVSYTIKAPSYMIKVDKQAYYADAMS
                     ICKNLLPSTQTVLSDIYDSWGAANKYSHYSSMNSITAWIKQTSSEQRSGVSSTYNLIT
                     QNPLPGVNVNTPNVYAVCVE"
BASE COUNT          951 a          611 c          732 g          910 t
ORIGIN      
        1 gaatatatga tgatttatgg tgtctgtggg aaattcccga cagataactc taacttcgct
       61 cttgagattt tgaatgcaaa cttatggttt gcagagaatg gtgggccata tctgtgctat
      121 gaggctggag cacaatcgct gttgttagcg ttacgtttcc ctctcgatga tgctacccct
      181 gaaaaactcg agaatgaaat agaagtcgtt gttaagtcaa tggaaaacct gtatttggta
      241 ttacataatc agggaataac attagaaaac gaacatatga aaatagagga aatcagttca
      301 agcgacaata aacattatta cgccggaaga taaaatccga tctattaata taatttattt
      361 ctcattctaa ctcattgtgg tggagccata acatgattac tcatggttgt tatacccgga
      421 cccggcacaa gcataagcta aaaaaaacat tgattatgct tagtgctggt ttaggattgt
      481 ttttttatgt taatcagaat tcatttgcaa atggtgaaaa ttattttaaa ttgggttcgg
      541 attcaaaact gttaactcat gatagctatc agaatcgcct tttttatacg ttgaaaactg
      601 gtgaaactgt tgccgatctt tctaaatcgc aagatattaa tttatcgacg atttggtcgt
      661 tgaataagca tttatacagt tctgaaagcg aaatgatgaa ggccgcgcct ggtcagcaga
      721 tcattttgcc actcaaaaaa cttccctttg aatacagtgc actaccactt ttaggttcgg
      781 cacctcttgt tgctgcaggt ggtgttgctg gtcacacgaa taaactgact aaaatgtccc
      841 cggacgtgac caaaagcaac atgaccgatg acaaggcatt aaattatgcg gcacaacagg
      901 cggcgagtct cggtagccag cttcagtcgc gatctctgaa cggcgattac gcgaaagata
      961 ccgctcttgg tatcgctggt aaccaggctt cgtcacagtt gcaggcctgg ttacaacatt
     1021 atggaacggc agaggttaat ctgcagagtg gtaataactt tgacggtagt tcactggact
     1081 tcttattacc gttctatgat tccgaaaaaa tgctggcatt tggtcaggtc ggagcgcgtt
     1141 acattgactc ccgctttacg gcaaatttag gtgcgggtca gcgttttttc cttcctgcaa
     1201 acatgttggg ctataacgtc ttcattgatc aggatttttc tggtgataat acccgtttag
     1261 gtattggtgg cgaatactgg cgagactatt tcaaaagtag cgttaacggc tatttccgca
     1321 tgagcggctg gcatgagtca tacaataaga aagactatga tgagcgccca gcaaatggct
     1381 tcgatatccg ttttaatggc tatctaccgt catatccggc attaggcgcc aagctgatat
     1441 atgagcagta ttatggtgat aatgttgctt tgtttaattc tgataagctg cagtcgaatc
     1501 ctggtgcggc gaccgttggt gtaaactata ctccgattcc tctggtgacg atggggatcg
     1561 attaccgtca tggtacgggt aatgaaaatg atctccttta ctcaatgcag ttccgttatc
     1621 agtttgataa atcgtggtct cagcaaattg aaccacagta tgttaacgag ttaagaacat
     1681 tatcaggcag ccgttacgat ctggttcagc gtaataacaa tattattctg gagtacaaga
     1741 agcaggatat tctttctctg aatattccgc atgatattaa tggtactgaa cacagtacgc
     1801 agaagattca gttgatcgtt aagagcaaat acggtctgga tcgtatcgtc tgggatgata
     1861 gtgcattacg cagtcagggc ggtcagattc agcatagcgg aagccaaagc gcacaagact
     1921 accaggctat tttgcctgct tatgtgcaag gtggcagcaa tatttataaa gtgacggctc
     1981 gcgcctatga ccgtaatggc aatagctcta acaatgtaca gcttactatt accgttctgt
     2041 cgaatggtca agttgtcgac caggttgggg taacggactt tacggcggat aagacttcgg
     2101 ctaaagcgga taacgccgat accattactt ataccgcgac ggtgaaaaag aatggggtag
     2161 ctcaggctaa tgtccctgtt tcatttaata ttgtttcagg aactgcaact cttggggcaa
     2221 atagtgccaa aacggatgct aacggtaagg caaccgtaac gttgaagtcg agtacgccag
     2281 gacaggtcgt cgtgtctgct aaaaccgcgg agatgacttc agcacttaat gccagtgcgg
     2341 ttatattttt tgatcaaacc aaggccagca ttactgagat taaggctgat aagacaactg
     2401 cagtagcaaa tggtaaggat gctattaaat atactgtaaa agttatgaaa aacggtcagc
     2461 cagttaataa tcaatccgtt acattctcaa caaactttgg gatgttcaac ggtaagtctc
     2521 aaacgcaagc aaccacggga aatgatggtc gtgcgacgat aacactaact tccagttccg
     2581 ccggtaaagc gactgttagt gcgacagtca gtgatggggc tgaggttaaa gcgactgagg
     2641 tcactttttt tgatgaactg aaaattgaca acaaggttga tattattggt aacaatgtca
     2701 gaggcgagtt gcctaatatt tggctgcaat atggtcagtt taaactgaaa gcaagcggtg
     2761 gtgatggtac atattcatgg tattcagaaa ataccagtat cgcgactgtc gatgcatcag
     2821 ggaaagtcac tttgaatggt aaaggcagtg tcgtaattaa agccacatct ggtgataagc
     2881 aaacagtaag ttacactata aaagcaccgt cgtatatgat aaaagtggat aagcaagcct
     2941 attatgctga tgctatgtcc atttgcaaaa atttattacc atccacacag acggtattgt
     3001 cagatattta tgactcatgg ggggctgcaa ataaatatag ccattatagt tctatgaact
     3061 caataactgc ttggattaaa cagacatcta gtgagcagcg ttctggagta tcaagcactt
     3121 ataacctaat aacacaaaac cctcttcctg gggttaatgt taatactcca aatgtctatg
     3181 cggtttgtgt agaataattc cata
//
LOCUS       DBFIFI010000316         3183 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.318, whole genome shotgun sequence.
ACCESSION   DBFIFI010000316 DBFIFI010000000
VERSION     DBFIFI010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.318"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            128..310
                     /locus_tag="N7B39_004687"
     CDS             128..310
                     /locus_tag="N7B39_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424511.1"
                     /translation="MEQTGRLFKQRRLSTTWLKSQITQPHKLWDAMPKQPSQEELRDC
                     IAKVYSGGIYVQKNRI"
     gene            288..560
                     /locus_tag="N7B39_004688"
     CDS             288..560
                     /locus_tag="N7B39_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein N"
                     /protein_id="HGU2424512.1"
                     /translation="MSRKTEFKGTAASRRRARRANLQSQEAISSDKLHRPTPSRVVLQ
                     CKRKPAMRAEVITLTTLTRKYEGSTCLPNVALYAAGYRKSKQLTAR"
     gene            619..870
                     /locus_tag="N7B39_004689"
     CDS             619..870
                     /locus_tag="N7B39_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424513.1"
                     /translation="MEFHESAICDFRANANSVKPQPIAVLFKTMGAWAVLCFAADDTD
                     ARMAIGQEMEMDPTNDEFIIYGAPSNYLLDTCNIYNKAA"
     gene            1053..1421
                     /locus_tag="N7B39_004690"
     CDS             1053..1421
                     /locus_tag="N7B39_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2528 family protein"
                     /protein_id="HGU2424514.1"
                     /translation="MSNIKKYIIDYDWKASIEIEIDHDVMTEEKLHQINNFWSDSEYR
                     LNKHGSLLNAVLIMLAQHALLIAISSDLNAYGVVCEFDWNDGNGQEGWPPMDGSEGIR
                     ITDIDTSGIFDSDDMTIKAA"
     gene            1494..1658
                     /locus_tag="N7B39_004691"
     CDS             1494..1658
                     /locus_tag="N7B39_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HGU2424515.1"
                     /translation="MQYAIAGWPVAGCPSESLLERITRKLRDGWKRLIDILNQPGVPK
                     NGSNTYGYPD"
     gene            1627..1770
                     /gene="kil"
                     /locus_tag="N7B39_004692"
     CDS             1627..1770
                     /gene="kil"
                     /locus_tag="N7B39_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HGU2424516.1"
                     /translation="MDQTLMAIQTKFTIATFIGDEKMFREAVDAYKKWILILKLRSSK
                     SIH"
     gene            1845..2141
                     /locus_tag="N7B39_004693"
     CDS             1845..2141
                     /locus_tag="N7B39_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HGU2424517.1"
                     /translation="MNAYYIQDRLEAQSWTRHYQQIAREEKEAELADDMGKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            2147..2932
                     /gene="bet"
                     /locus_tag="N7B39_004694"
     CDS             2147..2932
                     /gene="bet"
                     /locus_tag="N7B39_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HGU2424518.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYNKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKVLGFLKQKASEQKVAA"
     gene            2929..>3183
                     /locus_tag="N7B39_004695"
     CDS             2929..>3183
                     /locus_tag="N7B39_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HGU2424519.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYE"
BASE COUNT          954 a          747 c          745 g          737 t
ORIGIN      
        1 cacgctaaaa aacaatacta ttaaatacaa agcgttgtaa aaaaacgccc cgttttagaa
       61 caaattgtat tgaccaagtg aagtacatat cgtactattt aaccatcagc aggacgctgg
      121 aagccaaatg gaacagactg gcaggctctt taaacaacgt cgactctcga ctacgtggct
      181 gaaaagccag atcacccaac cacataagct gtgggatgca atgccgaagc aaccgtctca
      241 ggaggagctt cgagattgca tcgccaaagt ttattcggga ggaatctatg tccagaaaaa
      301 cagaatttaa aggcaccgca gcttctcgcc gtagagctcg tcgcgcaaac ctgcaaagtc
      361 aggaggcgat tagctccgac aaactacaca ggccaacccc atcacgagtg gtcttacaat
      421 gcaaacgcaa accagcaatg agagcagaag taataacact gacaacgttg accagaaaat
      481 atgaaggctc aacttgtctt ccaaatgtag ctctttacgc ggcaggctac cgtaaatcca
      541 aacaactgac agcaagatga cttgtgttgg tcgccagaaa atgaaattag gcagcaaacc
      601 acttatttga ggtgatatat ggaatttcat gaaagtgcga tttgtgattt tcgcgctaac
      661 gcaaattcag taaaaccaca gccaattgca gttcttttta aaacaatggg tgcgtgggct
      721 gttttatgct tcgccgctga cgacactgac gcaagaatgg caataggaca agagatggag
      781 atggacccga caaacgatga attcataatt tatggcgctc catctaatta cttacttgat
      841 acctgcaaca tttacaacaa ggctgcctga tggtggcctt tatttttggc ataaacaaca
      901 gaataaacac agcactgtgt attcattcca acgagtgaat acacggagca atgtcgctcg
      961 taactaaaca ggagccgact tgttctgatt attggaaatc ttctttgccc tccaatgtga
     1021 gggcgatttt ttatctgtga ggatatgaac agatgtcaaa catcaaaaaa tacatcattg
     1081 attacgactg gaaagcatca atagaaattg aaatcgacca tgacgtaatg acagaggaaa
     1141 aacttcacca gattaataat ttctggtcag actctgaata ccgactcaat aaacacggct
     1201 ctttattaaa tgctgtatta atcatgctgg cgcaacatgc tctgcttata gcaatttcaa
     1261 gcgacttaaa tgcatatggt gttgtgtgtg agttcgactg gaatgatgga aatggtcagg
     1321 aaggatggcc tccaatggat ggtagcgaag gaataagaat taccgatatc gatacatcag
     1381 gaatatttga ttcagatgat atgactatca aagccgcctg agcgcggcgt taccgcatac
     1441 caataacgct tcactcgagg cgtttttcgt tatgtataaa taaggagcac accatgcaat
     1501 atgccattgc agggtggcct gttgctggct gcccttccga atctttactt gaacgaatca
     1561 cccgtaaatt acgtgacgga tggaaacgcc ttatcgacat acttaatcag ccaggagtcc
     1621 caaaaaatgg atcaaacact tatggctatc cagactaaat tcactatcgc cacttttatt
     1681 ggcgatgaaa agatgtttcg tgaggccgtc gacgcttata aaaaatggat attaatactg
     1741 aaactgagat caagcaaaag cattcactaa ccccctttcc tgttttccta atcagcccgg
     1801 catttcgcgg gcgatatttt cacagctatt tcaggagttc agccatgaac gcttattaca
     1861 ttcaggatcg tcttgaggct cagagctgga cgcgtcacta ccagcagatc gcccgtgaag
     1921 agaaagaggc agaactggca gacgacatgg gaaaaggtct gccccagcac ctgtttgaat
     1981 cactctgcat cgatcatttg caacgccacg gggccagcaa aaaagccatt acccgtgcgt
     2041 ttgatgacga tgttgagttt caggagcgca tggcagaaca catccggtac atggttgaaa
     2101 ccattgctca ccaccaggtt gatattgatt cagaggtata aagcggatga gtacagcact
     2161 cgcaacgctg gcagggaagc tggctgaacg tgtcggcatg gattctgtcg acccacagga
     2221 actgatcacc actcttcgcc agacggcatt taaaggtgat gccagcgatg cgcagttcat
     2281 cgcattgttg atcgtcgcca accagtacgg ccttaatccg tggacgaaag aaatttacgc
     2341 cttccctgat aagcagaacg gcatcgttcc ggtggtgggc gttgatggct ggtcccgcat
     2401 catcaatgaa aaccagcagt ttgatggcat ggactttgag caggacaatg aatcctgtac
     2461 atgccggatt taccgcaaag accgtaatca tccgatctgc gttaccgagt ggatggatga
     2521 atgccgccgc gaaccattca aaacccgcga aggcagagaa atcaccggcc cgtggcagtc
     2581 gcatcccaaa cggatgttac ggcataaagc catgattcag tgtgcccgtc tggccttcgg
     2641 atttgctggt atctataaca aggatgaagc cgagcgcatt gtcgaaaata ctgcatacac
     2701 tgcagaacgc cagccggaac gcgacatcac tccggttaac gatgaaacca tgcaggagat
     2761 taacactctg ctgatcgccc tggataaaac atgggatgac gacttattgc cgctctgttc
     2821 ccagatattt cgccgcgaca ttcgcgcatc gtcagaactg acacaggccg aagcagtgaa
     2881 agttcttgga ttcctgaaac agaaagcctc tgagcagaag gtggcagcat gacaccggac
     2941 attatcctgc agcgtaccgg aatcgacgtg agagctgtcg aacaggggga tgatgcatgg
     3001 cacaaattac ggctcggcgt catcaccgct tcagaagttc acaacgtgat agcaaagccc
     3061 cgctcaggaa agaagtggcc tgacatgaaa atgtcctact tccacaccct gcttgccgag
     3121 gtttgcaccg gtgtggctcc ggaagttaac gctaaagcac tggcctgggg aaaacagtac
     3181 gag
//
LOCUS       DBFIFI010000317         3172 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.319, whole genome shotgun sequence.
ACCESSION   DBFIFI010000317 DBFIFI010000000
VERSION     DBFIFI010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.319"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(601..798)
                     /locus_tag="N7B39_004696"
     CDS             complement(601..798)
                     /locus_tag="N7B39_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU2424520.1"
                     /translation="MLTTTSHDSVFLRADNSLIDMNYITSFTGMTDKWFYKLISEGHF
                     PKPIKLGRSSRWYKSEVEQWK"
     gene            complement(864..1134)
                     /locus_tag="N7B39_004697"
                     /pseudo
     CDS             complement(864..1134)
                     /locus_tag="N7B39_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593693.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1439..1627
                     /locus_tag="N7B39_004698"
     CDS             1439..1627
                     /locus_tag="N7B39_004698"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424521.1"
                     /translation="MTNEELQAIALSMLERQREKVHQQRESLLNPSPFTRPKRLELEA
                     FLDKYPRRLKSGKTRPPG"
     gene            1955..2149
                     /locus_tag="N7B39_004699"
     CDS             1955..2149
                     /locus_tag="N7B39_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424522.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            2492..2620
                     /locus_tag="N7B39_004700"
     CDS             2492..2620
                     /locus_tag="N7B39_004700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424523.1"
                     /translation="MGADVTEFSVQGKSGTCRQFSDLFNREIISYSLSERSVMEHR"
     gene            complement(2930..>3172)
                     /locus_tag="N7B39_004701"
     CDS             complement(2930..>3172)
                     /locus_tag="N7B39_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424524.1"
                     /translation="IIQGKAISSVGRNVEISTGKRGTDINTRRIPASNCLSARNAELK
                     HNHTGARILWSYAFSIVTTNRNKNLAGANIKRCDTV"
BASE COUNT          935 a          731 c          703 g          803 t
ORIGIN      
        1 cagtttgtag gagctttctt ttttgaacaa tgatttgcag gacagttatg ctaaactagc
       61 ggaatcagta tcacaacaat aaatgactga tcatacctga aatttgaatt aacaggcatc
      121 atgaatgtca atagtctaag cggtgactac ttatatcgct tgcataaata ctaaacactg
      181 actagaaaag tattggataa ccccgtcact aaaagtaacg cttcggttat ttgctcggaa
      241 tgtattaacg ccaagattgt tagccgttac gccttatatc actggcgctg acaaatgttt
      301 cttttctaat ttactgaacc ggcgcagaca cgcaggaaag cacgccggca ccggtgtaat
      361 gccagttcta atcaataaga atggaatgac gaatcagtca ggaagggata acaacccgct
      421 gctgaacgga aggtatcagc aggaagatga acgggaatat cgccagcacc aagggcaggg
      481 ccgggttcca caataccagc gctggcattt tgcacattgc cagcgtaccg gcattacaac
      541 acgtttcatg ctgctgcccc ccaggattcc tcgattcgtt gctacatcca ctgctctact
      601 tcacttccac tgctccactt cacttttgta ccagcggctg ctgcgcccca gtttgatggg
      661 tttagggaaa tggccttcac tgatcaactt gtaaaaccat ttgtcggtca taccggtgaa
      721 actggtgata tagttcatgt cgatcaggga attatcggca cgcagaaata cgctgtcgtg
      781 gcttgttgta gtcaacatgg tcagaatgtc ctgtgagatt tatgaagatt gtgcgaatgc
      841 gggaaatcta ctgagctgtg ctttcagaac tggcctgtta cgggatacca gggattaccg
      901 gcggggtaac gggcttccgg atcatacaca ccacgattat cgcggacaaa atcactgaac
      961 gcccatatca cctctttaag tatgtcttcg cagtccggta aatgacgatc cagcgccaca
     1021 tcccgggtgg tacagctttg atgcgcccgg tgacacaaag cccggattgt tccagacatc
     1081 ctgaatcaaa cgccccagat taggggcgtc gaaatatgcc tctctgacca ttatattccg
     1141 gcgtataggt agcaggtctg aagtgacaat gcgtcacctg acgttaaaag tcacccaaga
     1201 tgaccccagt gggggcaaca agtataaaac cgttttttga ttaagtggtc ggatatgaaa
     1261 aggggaaggt ttatgtgaaa aatacctgtg ttttctttga aaacacacaa cagtatcagg
     1321 ttatttatgt ttatacagtg cgttccaaac tgacctgaat aattacgcaa gttgcaacaa
     1381 taaaacattt ttgccgtagt gtgaggtact ccggtaaatt gcttcccagg tgcagacaat
     1441 gactaacgaa gaactacagg caatcgcatt atccatgctt gagcgccagc gcgaaaaagt
     1501 acaccagcaa cgagagtctt tgcttaaccc atctccattc accaggccaa aacgattgga
     1561 gcttgaagca ttcttggaca aatacccacg gcggcttaaa tcaggcaaaa ccagaccacc
     1621 aggctgatag ctcacagttt gttctccgat tcaataaaac ggataagaac catcagcgcg
     1681 gtagcgtaag gcactacagc gcattgcttt acgagtaata acaatggatt tatcaccaga
     1741 agacgcaaag tttttacgag aactgcaaca ccgtatgctc agagaagcca ttatcgaaca
     1801 gcaaacgggc atgagcgcca tagacccatt gcgatacaaa cgggcctcat ctcctttagt
     1861 tgacgacaat tccaatgcgg ataaaacacg tccaccacac tgattacatt tttcattagc
     1921 acagtggtgt gatgcagcat acgaggaaaa ccggatgaat gagaacaaaa taaaaaggct
     1981 ggagcaactg ttacaggcac gccaacggga gtttgccact aaatggggac gtgccgcgct
     2041 gccacatgaa cgcctaacct attttccact tgaagacctc attaaaaata atggcaaaaa
     2101 aatcaggtct gataagaaac acttcttaga atgcaatgaa gacaaataac aacaatctgc
     2161 atattgccac caaccacaga tgtggcatcg ttatcagcgc acttgatttt ctttatatcc
     2221 actgacgagt ttgccggtca ttctggtatc atgcagatat ttacggcgta caagcacact
     2281 agcatgacat tgtaataagg cctgagccta acgctttaat gtgtgtcata cactcagtgg
     2341 cgtaatcgtt gacaatatgg tgcatgtaat cgtaagcgtc cagcgaaatc catctggtca
     2401 taatctgaag catccgacaa agtggtgtcc accaaataag tgatctgcgc ccaatatcct
     2461 gagccggaac tttgacaaag gccaacgaaa aatgggagcg gatgttacag agttctcggt
     2521 acaggggaaa agtggtacct gtcgccagtt ctccgatctg tttaaccggg aaattatctc
     2581 ctacagcttg tcagaaaggt cggtgatgga gcatcgttaa tacgatgcta tacgatgcat
     2641 tcacagtgcc cggccagagg atgccccgtc gctgcatatg gatcagggtt ggcaatatcg
     2701 aattgcaggc tataggcaaa gttaaagccc catggagtag cacaaaatat gccgcacaaa
     2761 ggaaacggtc tgaataacgc agtgatgaag aacttcttca gcacactgct aaaacgcata
     2821 gtgatcgagc gctgattctg gcgaacaact gaactaatac atcagaatct gcattatgtt
     2881 aaataaatat aaaaagatgg tttaaatacc ccgttacttt tgacttacac tatacggtat
     2941 cgcatcgttt aatattcgca ccggccagat ttttatttct attagttgtc acaatactga
     3001 atgcgtacga ccacagtatt ctggctcctg tgtggttatg ctttaattct gcgttccggg
     3061 cagataagca gttgcttgca ggaatccttc ttgtgttaat gtcagttccc cttttaccag
     3121 tgctgatttc cacattccgt ccaacagagc ttatagcctt tccctggatt at
//
LOCUS       DBFIFI010000318         3159 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.320, whole genome shotgun sequence.
ACCESSION   DBFIFI010000318 DBFIFI010000000
VERSION     DBFIFI010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.320"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..315
                     /locus_tag="N7B39_004702"
     CDS             <1..315
                     /locus_tag="N7B39_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU2424525.1"
                     /translation="EQLSKKFRASVSYICHEFTKEYRISPINYVIQRRMTEAKWSLTN
                     TELSQAEISWRVGYENVDHFAKLFLRHVGCSPSDYRRQFKNCFAEQEILSEFPQPVSL
                     AG"
     gene            complement(358..1509)
                     /locus_tag="N7B39_004703"
     CDS             complement(358..1509)
                     /locus_tag="N7B39_004703"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HGU2424526.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(1523..3118)
                     /locus_tag="N7B39_004704"
     CDS             complement(1523..3118)
                     /locus_tag="N7B39_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HGU2424527.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SDLAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQSQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEIADGKLNIVQEGRVKKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
BASE COUNT          784 a          809 c          719 g          847 t
ORIGIN      
        1 gaacagttat cgaaaaaatt tcgtgccagc gtcagttata tttgccatga atttaccaaa
       61 gagtatcgta tttcccctat taactatgtt attcaacggc gtatgacgga agcaaaatgg
      121 tcactcacta atactgaatt atcacaggca gagatctcct ggcgtgtggg ttatgaaaat
      181 gtcgatcact ttgccaaact gtttttgcgc catgtcggct gttcgcccag cgattaccgc
      241 aggcaattta aaaactgttt tgcggaacaa gaaatcctat ctgaatttcc tcaaccggta
      301 agtcttgccg gataaatctg gcggcgcagt ttgttaagaa gctgcgccgc ctgcagatta
      361 tttgttctga tagtctttca ggatctgccg acctgctacg taaatcataa tttcgtctgt
      421 accgccgcca atacgttcac aacggacatc acgccagaag cgggagacgc gcgcctcatc
      481 ggtatagccc agaccgccca tgatttgaat cgcatcatca atgacttcca ttgcggtacg
      541 tgcacagtac agttttgcca gcgccgcgct ggtgcgcagt gattgatgct gatcggcttg
      601 ccatgccact ttcagcacca tgttgcgcat gttgtcaatc ttaatcgcca tcagcgccag
      661 tttttcctgg atcatctggt tatgaccaat gggtttacca aaagcgatac gctggttggc
      721 atagcgagcg gcatcttcaa aggcacactc ggcaaagccg gtgctgcgcg cggcgttgat
      781 caggcgctcc atctcaaagt tgtacatcac attgaggaaa cccattcctt cttcgcccac
      841 catgtcgctc tcttcaactt ccacgttgtc gagatagact tcgcaggtgc tgagcatatg
      901 ccagccgatt ttatgcagcg ggttaatctt aatgccgggc ttactggagt cgacccacca
      961 cagggtgaag gcttttttgg gatctttcgg ttgcggatcg cgcgccagca ccagcatata
     1021 cgggtactct ttcgcaccgg taataaaggt tttctgtccg ttgatgtaaa ccttgccgtt
     1081 tttacgcgtg taagtggtag tggcactgtt gttatctgag ccagcacctg gttccgtcaa
     1141 cgccagggca taggcgggat cacccgtttc cagggtgctt tctgccgttt tacgtagctg
     1201 ctctgcagaa ccgaaacggc gcatactgtg aatacattga ccgttggtaa ttaaaaatgc
     1261 cggagcaccg cattttgaca cttccatcag cgccagcatt tgggtgacgt aatcagcagg
     1321 gataccacca aattcttccg gcacgccaag catggaaata ccgttatccg ccagtgcccg
     1381 cataaactca cgcggatatg tcccgttttg atcgcaggtg cggaaatact cttccggaaa
     1441 gttagtcgta atcagttcgc gaatactggc cagcagcagt tcttgttctt cagttaaaga
     1501 aaaatccatc gtgatactcc tgttaatgag ccgcttcagg caggacaaaa cccatcgccg
     1561 catcgataaa taatctttcg tccatcagtt tgagttctgg cgaaatcact ggggtgaaat
     1621 ccattttgtc gagaatatct ttttgtaaat cgacgccagg ggcgatttcg attaaatgca
     1681 gtccgtcttc tttcagcgtg aatacggcgc gctcagtgat ataacgaaca tccagccctc
     1741 gctcgagagc gatttttccg ctaaaagtaa tttccggtag ttcccgaata aatttcttca
     1801 cccgtccttc ctggacgata ttcaatttgc catcggcaat ttctgttttt aaactgcccg
     1861 cagttaacgt gccgcagaaa ataattttct tcgaggtggc actgatatca ataaatccac
     1921 cggtacccat gattttgcca ttgaatttat gcacgccgac gttaccgtgc tggtcgactt
     1981 cagcaaaact caaataacaa acatccagac caccaccgtg ataaaaatca aactgggacg
     2041 tcatatccag aatggcacgg gtattcacgt tcgcgccaaa ggcgataccc tgtgaagtaa
     2101 ttccaccaat cggacctgtt tctaccgtca gaataaagtc atcagcacaa ccttcttccc
     2161 gggcgaccag gccaatgccg tcagcaatac cgacgccgac attccccacc gcgcctttac
     2221 gcatttcgaa taatgcacgc cgtgcaacta atttacgttg gtttaggggc aggctaagtt
     2281 tggtgctgtc atcaagggtg aagtcaccag aaataaagcg gttaaccggc gcaccgccat
     2341 atagttggct ttgatccgga tcgaccacca caatatccac cagataaccc ggaatacgga
     2401 cagatttagg atgcagtgtg gctttcttaa ccattttctg cacctgcatc atcacaatac
     2461 cgccgttatt gtgcaccgcc tgggcaataa ccaatgcgtc gagatacatc acctcatctt
     2521 caaaagtggc gtagccttca ctgtcgcagg tggtagcgcg aatgaaagca atatctggcg
     2581 caatcgcttt gtaatagaga tattctttgt tatcaaactc gaccagttta atcaggtctt
     2641 ctttagtgac ttcattcagt ttgccgcctt gctggcgtgg atcgacaaat gtcccgatgc
     2701 caatatcact aataatacca ggctggtggg ctgcggcggc gcgtaaggtt tgtgtaagta
     2761 caccttgtgg atagttataa gcaataattt tattttgttc tgcgagatca gaaatacgcg
     2821 gcgattgtcc ccagtgaccg cataatgccc atttcaccag accttcttgc gccagcggac
     2881 taataccgcg gtcggcgcga tcgccaagcc ctgttggact aataatcgat aaattacgtg
     2941 gcgtttgagt ctgtttatat ttatcagcaa gagcagtaat taacgtggtg gcttccagaa
     3001 taccgccgcc agcgcctaac acacaaagtg ttgcttcgtc gggaatataa ttcaccgctt
     3061 cctgtgccga caggaccggc actcgtccat taatacgagg tggttttaca ggtttcattg
     3121 ttaattcctg attcaggtgt gattttccct acgccagat
//
LOCUS       DBFIFI010000319         3139 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.321, whole genome shotgun sequence.
ACCESSION   DBFIFI010000319 DBFIFI010000000
VERSION     DBFIFI010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.321"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..608)
                     /gene="dsbA"
                     /locus_tag="N7B39_004705"
     CDS             complement(<1..608)
                     /gene="dsbA"
                     /locus_tag="N7B39_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HGU2424528.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVK"
     gene            complement(625..1611)
                     /gene="srkA"
                     /locus_tag="N7B39_004706"
     CDS             complement(625..1611)
                     /gene="srkA"
                     /locus_tag="N7B39_004706"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HGU2424529.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(1688..1957)
                     /locus_tag="N7B39_004707"
     CDS             complement(1688..1957)
                     /locus_tag="N7B39_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HGU2424530.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            2027..2611
                     /gene="mobA"
                     /locus_tag="N7B39_004708"
     CDS             2027..2611
                     /gene="mobA"
                     /locus_tag="N7B39_004708"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HGU2424531.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIAMVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            2593..3120
                     /gene="mobB"
                     /locus_tag="N7B39_004709"
     CDS             2593..3120
                     /gene="mobB"
                     /locus_tag="N7B39_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HGU2424532.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQNQDG"
BASE COUNT          821 a          765 c          759 g          794 t
ORIGIN      
        1 tatttcactg tatcagcata ctgctgaaca aaaacatcca tattgctggt atccataccc
       61 tgcggattca gctgatattt accgttaaca aacatcgccg gaacaccacg caattgcacg
      121 tcagctgcag ctttttcctg ctgagcgacc agagatttca ccacgaagct gttccacgcc
      181 gcgtcgtact cttcaccttt aatacctgcg ttgataaata catcgcggat atcagatgct
      241 gaacgaatgg tctgggtttt ctgtacgcct tcaaacagcg gaacagtcac tttgtcttcc
      301 acgcccagcg ccatcgccac agcccatgcc tgagtcagat ctttgcccag gtcaccaccc
      361 atgaagttga cgtggtattt agtcatcttc acgccttccg gcagtttttt cttcacatta
      421 tcagaaatat gcagaacttc ttcaaactga tagcagtgcg ggcagaagaa agagaaaaac
      481 tccagcactt gcggcgcgcc agctaccggt ttttccaggg tagtgtactg tttaccatct
      541 tcatactgcg ccgccgatgc gctaaacgct aaaactaaac cagccagcgc cagccaaatc
      601 tttttcatga tctactctct ccgattaata cataggtgtt aattgcaaag ggggttcttg
      661 tagaactttt gcctgttcta taaaagtcgc cgtctgtcgt agccagtaat cttccccggt
      721 taaccacggg aaatttttcg ggaacgcggg atcagcccaa cgccgcatta gccaggcaag
      781 ataataaacc aaacgcatgg cgcgtaaagg ttcaatcagt ccgatttcag cggtgtcgaa
      841 ctcgctaaat tcttcataag cttcaataat agtttccagt tgcatccgct gctcggcttt
      901 atcgccattg agcaacatcc acaaatcctg aatagctgga ccattacgtg catcatccag
      961 atcaacaaac attggaccat cgcgccagag aatattcccg gcgtggcagt ctccatgtag
     1021 ccgcagaacg gtgaaatctt cccgccagtg tgctgtaacg gcggcgatca gctcatctgt
     1081 cgctttcagg aatgccgctt tcaacccgga aggtatcagt gtagcgtcct caaacagctt
     1141 gcgtggctca atgagatatt cattcagacc gatggtcggg cgatggataa aaagctgttt
     1201 gcgccccgtc tggtgcatac gccctaaata acgcccaacc gcttccatct gatcgatatt
     1261 atcagcttcg aactggcgac caccgacgct tggaaaaaca gcgaaataaa atccctgatg
     1321 attcaataaa gtctgaccgt taaaagccac aggtgctgca accggaactt catcatttac
     1381 cagctgcaac gcaaattgat gttcttcgag gatttgatcg gctgtccaac gttcaggacg
     1441 ataaaatttg acgacaaaac gtcgacgatc ttcgtcctga aattgataga cacggttttc
     1501 atagctgtta agcggggtaa gaccggaatc cacccggatc ccttgctcaa acagagcgtc
     1561 catgatggta tccgggtgta gtgtctggaa agtaaaagcg ctgttgttca tcccatcatc
     1621 cggaaaacat tacgaatgat gaaggatatc atttcacgat gattttggtg gcgccgctta
     1681 caagctttta ctctttaatt acgccgcgag cgcgtaacag cgccgttttg aagtcttctt
     1741 cataatcttt ctgcaaaccc gggatcaccg catctttcgc ggaatcacgc attttcaggt
     1801 gatagatcag aatgtcatcc gtcaaatccg ccagttcgcc gtcaaaacct gactctttcg
     1861 ccagtttctg caaaaattgc aacaggttaa ggtctggctc tttttgccag gctggctgga
     1921 ggagttcaat aacttcattc agacgtttac atttcatagt aatgctcctt actcttgaga
     1981 cagacacgtt agcagggtca atcccacaat aaaagaggcg atatcggtga atctgatgac
     2041 gacgataaca ggcgttgtgc tggcaggcgg taaagccaga cgaatgggcg gcgtagataa
     2101 aggattgctg gacttaaatg gcaaaccgct gtggcaacat gtcgctgacg cgcttatgac
     2161 gcagctctct cacgtcgtgg ttaatgctaa tcgtcatcag gaaatctatc aggcaggcgg
     2221 tctgaaagtg attgaagatt cactggcgga ttacccaggc cctctggcag ggatgctttc
     2281 agtaatgcag caggaagctg gtgagtggtt tttgttttgt ccgtgcgata cgccttacat
     2341 tccccatgat ttagccgccc ggcttaatca tcagcgcaaa gatgcgcctg tcgtgtgggt
     2401 ccatgacggt gaacgcgatc acccgactat tgctatggta aaccgcgcta ttgagccttt
     2461 attactagaa tatctgcaag caggagaacg ccgggtaatg gcatttatgc gtctggctgg
     2521 cggtcatgcg gttgatttca gcgatcataa agatgcattt gttaacgtga atacgccaga
     2581 ggagcttgcc cgatggcagg aaaaacgatg ataccgttac tcgcctttgc cgcgtggagt
     2641 ggtaccggaa aaactacgct tttgaaaaaa ctgatcccgg cattatgcgc cagagggatc
     2701 cgtccagggc tgattaagca tacgcaccat gatatggatg ttgataagcc aggcaaagat
     2761 agctatgagc tgcgcaaggc tggcgcggca caaaccatcg ttgccagcca gcaacgatgg
     2821 gccttgatga cggaaacacc agacgaagaa gagctggatc tacagtttct cgcaagtcga
     2881 atggatacct caaagctgga tttgattctg gtcgaagggt ttaagcatga agagatcgca
     2941 aagattgtgc tgtttcgcga tggagccgga catcgacctg aagaattagt gatagacagg
     3001 catgttattg ctgtagccag tgatgtgccg cttaatcttg atgtcgcatt actggatatt
     3061 aatgatgttg aggggctggc tgattttgtg gtggagtgga tgcaaaatca ggacggataa
     3121 gcaagagtag gctggagca
//
LOCUS       DBFIFI010000320         3120 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.322, whole genome shotgun sequence.
ACCESSION   DBFIFI010000320 DBFIFI010000000
VERSION     DBFIFI010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.322"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            14..238
                     /locus_tag="N7B39_004710"
     CDS             14..238
                     /locus_tag="N7B39_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424533.1"
                     /translation="MCNATWPDAAVDAIKTLMDSLIEISAIAGVAHKHAARESECIFH
                     YLAFVQLKADQALDKAGKIIMADVQEVHHA"
     gene            231..824
                     /locus_tag="N7B39_004711"
     CDS             231..824
                     /locus_tag="N7B39_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424534.1"
                     /translation="MHNLSISDLNSIQFDEIFSGQLLVNVENGRVVSNYHLPDGAIAG
                     SVEALLELAERARLIKPSTCHHDDDLHFTGRMVSHYENGVEVSRERLRDDCCFGTLPE
                     FIELLTSCGYQVIQGGVNMRDDRFNSLKREFDGAPEDAAGALLSVADMMKAAYFLINT
                     SGYKSEGEMILSIASDYAEYVAETRYRRKFTEDVSHA"
     gene            817..1016
                     /locus_tag="N7B39_004712"
                     /pseudo
     CDS             817..1016
                     /locus_tag="N7B39_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551489.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1021..1263
                     /locus_tag="N7B39_004713"
     CDS             1021..1263
                     /locus_tag="N7B39_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001723122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HGU2424535.1"
                     /translation="MKKKNSGFTASGLSRPEIRLGDIYRDTRRGGRVVIRHVTPGNIT
                     YRRDFTLVDHYKAVNQKRAASYIRKIREMLVAGGKK"
     gene            1260..3074
                     /locus_tag="N7B39_004714"
     CDS             1260..3074
                     /locus_tag="N7B39_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase-like DNA-binding domain-containing
                     protein"
                     /protein_id="HGU2424536.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWAHAKQWQEHDARMA
                     GDNEPPVVLADEQLKEIGNLQIVPDGRTSARIFRAGQLDPVMVKAIGQKLAAASIQNA
                     NYYPDGMHSQKCENWREYLARERKNIDDGLIIRFPAKKKTEDSATPLALNQMGASQRG
                     EVLLAHYGGELAINADSDTVHHYNGVVWEPVQDKELQRTMAQIFIDAEISYSQNAIKS
                     AVDTMKLSLPVMGNAARNLIGFSNGVFDTRTGNFREHNKNDWLLIASELPFSPPAEGE
                     TLATHAPNFWKWLRRSVAENDRKADRVLAALFMVLANRYDWQLFLEVTGPGGSGKSVM
                     AEICTMLAGKANTVSASMKALEDARERALVVGFSLIIMPDMTRYAGDGAGIKAITGGD
                     KVAIDPKHKAPYSTRIQAVVLAVNNNAMSFSDRSGGISRRRVIFNFSEVVPENERDPM
                     LAEKIEGELAVVIRHLLTRFSDQDEAKRLLYEQQKSEEALVIKREGDSLVDFCGYLMS
                     SVMCDGLLVGNAEIIPFSPRRYLYHAYLAYMRAHGFGKPVTLTRFGKDMPGAMAEYGR
                     EYMKRKTKHGLRSNVILTEDSEDWMPSCASVTNDDGKN"
BASE COUNT          868 a          661 c          871 g          720 t
ORIGIN      
        1 cagggggtgg aacatgtgta atgcaacatg gcctgatgca gcggtagacg ctatcaaaac
       61 gctgatggat tcacttattg agatttctgc tatcgctggt gtggcgcata aacacgcagc
      121 cagagaatca gaatgcatct tccattattt agcatttgtg cagctaaaag ccgatcaggc
      181 actggataag gccggaaaaa ttatcatggc tgatgtgcag gaggtgcacc atgcataacc
      241 tgtcaatttc tgaccttaac agcattcagt ttgacgagat attttccggg cagctactgg
      301 tcaacgtgga gaatggacgc gtggtaagta attatcacct gccggatggt gcaattgccg
      361 gaagcgttga agcattgctg gaactggcgg aacgtgcgcg actgattaag ccgtcaacgt
      421 gccatcacga tgatgatctg cattttaccg gacgcatggt gagtcactac gaaaacggcg
      481 ttgaagtatc ccgcgaacgt ctgcgtgatg attgctgttt cggcacactg ccggaattta
      541 tcgaattgct gaccagttgc ggttatcagg tcattcaggg gggggtaaac atgcgtgatg
      601 atcgttttaa ttccctgaaa cgggaatttg atggcgcacc ggaagatgca gcgggcgcat
      661 tgttgagcgt tgctgacatg atgaaagctg catattttct tatcaatacc agtggctaca
      721 agtcagaggg tgaaatgatt cttagtattg cgtcggacta tgcggaatat gtggcagaga
      781 cgcgttacag aagaaaattc acggaggatg taagccatgc ataatcatga agcgcatgta
      841 cccgtagtgc ttaatgtgcc agatgatttc accggacgcg tactggttta cctggataaa
      901 gggaaagtga aatcacaatg ccgactgaaa agtaatgaga ttgttggttc tcctgaattt
      961 tttctgaact ctgtattcgt gcggaaataa aaccggaact gctgacagga aaataaaact
     1021 atgaaaaaga aaaattctgg ctttactgcc agcggtctct ctcggcctga aatcagactc
     1081 ggagatattt accgcgacac cagacgtggg ggacgagtgg ttattcgtca cgttacgcca
     1141 ggcaatatca cctaccgccg tgattttacg ctggtggacc attataaagc cgtaaatcaa
     1201 aaacgtgcag caagctatat ccgcaaaatc cgtgaaatgc tggttgcagg ggggaaaaaa
     1261 tgaaactggc accgaacgta aaaaaacagc cacgcggaat aaaacacaaa gacacagagg
     1321 taattatttt cgcgggtagt gatgcctggg cacacgcgaa acaatggcag gaacacgatg
     1381 cgcgtatggc tggagataat gagccacctg ttgtgctggc tgatgaacaa ctgaaagaga
     1441 tcggaaatct gcaaatagtg cctgatgggc gcacatccgc gcgcatattc agagccggac
     1501 agttagatcc tgtaatggtt aaggcaattg gtcagaagct ggcggcggca agtatacaga
     1561 atgcgaatta ttaccctgat ggcatgcaca gccagaagtg tgagaactgg cgtgaatacc
     1621 ttgcccgtga gcgtaaaaac attgatgacg gactgattat ccggtttccg gcaaagaaaa
     1681 aaacagaaga cagcgcgaca ccactggcac ttaaccagat gggagcaagc cagcgcggcg
     1741 aagttctcct ggcacattat ggcggtgaac tggcaatcaa tgcggattct gacaccgttc
     1801 accactacaa cggtgttgta tgggagccgg tacaggataa agagttacag cggactatgg
     1861 cgcagatttt tattgatgcg gaaatcagct attcgcagaa cgccatcaaa tcggcggtcg
     1921 ataccatgaa gttaagtttg cccgtaatgg ggaatgcagc ccgtaacctg attggattca
     1981 gtaacggggt atttgatacc agaactggta attttcggga gcataacaaa aacgactggt
     2041 tgttaattgc cagtgaatta cctttcagcc caccagcaga gggggaaacg ctggcaacac
     2101 atgcgccgaa tttctggaag tggttgcgcc gttcggtggc tgagaatgac cgcaaggcag
     2161 atcgcgtact ggcggcgtta ttcatggtgc tggcgaatcg gtacgactgg cagttatttc
     2221 ttgaggtaac ggggccgggt ggaagcggta aaagcgtaat ggcggagatt tgcactatgc
     2281 tggcgggtaa ggccaacacg gtatcggcaa gcatgaaggc gctggaagat gccagggaac
     2341 gtgcgttagt ggttggcttt tcgctgatta tcatgccgga tatgacccgt tacgctggtg
     2401 atggtgcagg gattaaggcc attacaggcg gtgataaggt ggcaatcgac ccgaaacata
     2461 aagcccccta ctcaacacgc attcaggcgg tcgtgctggc ggtgaacaat aacgccatgt
     2521 cattcagtga ccgcagcggt gggatatcac gtcgtcgggt aatattcaat ttttcggaag
     2581 ttgtaccgga gaacgaacgt gaccctatgc tggctgaaaa aatagaagga gagctggcgg
     2641 tagtgattcg ccacctgctt acacgctttt ctgaccagga cgaagctaaa cgcctgctat
     2701 atgagcagca gaaatcagaa gaagccctgg tgataaaacg cgagggcgat tcgctggtgg
     2761 acttctgcgg ctatctcatg tcgtcggtaa tgtgcgatgg cctgttagtg ggtaatgctg
     2821 aaattattcc gttcagcccg cgcaggtatc tctatcacgc ctatctggcg tatatgaggg
     2881 cacacggatt cggtaagccc gtaacactga cacgcttcgg taaggatatg ccaggggcaa
     2941 tggcggaata cggcagggag tatatgaaac ggaaaacgaa gcatggcttg cgttcaaacg
     3001 tgatactgac agaggattca gaagactgga tgccctcatg tgcatcggtc acaaatgacg
     3061 atggcaaaaa ttaaacttat ggaataactg ttcatcactg ttcaccctgt cataaatatc
//
LOCUS       DBFIFI010000321         3116 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.323, whole genome shotgun sequence.
ACCESSION   DBFIFI010000321 DBFIFI010000000
VERSION     DBFIFI010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.323"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            99..1619
                     /locus_tag="N7B39_004715"
     CDS             99..1619
                     /locus_tag="N7B39_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HGU2424537.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLVADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSTTVKQRVMAARERQFKRQNKLNAWLDSPEIRKFCKLESEDAQWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(1643..1981)
                     /gene="maoP"
                     /locus_tag="N7B39_004716"
     CDS             complement(1643..1981)
                     /gene="maoP"
                     /locus_tag="N7B39_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HGU2424538.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            2100..2939
                     /gene="hdfR"
                     /locus_tag="N7B39_004717"
     CDS             2100..2939
                     /gene="hdfR"
                     /locus_tag="N7B39_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HGU2424539.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNTLIRDLLKINVLD
                     EVY"
     gene            complement(3035..3110)
                     /locus_tag="N7B39_004718"
     tRNA            complement(3035..3110)
                     /locus_tag="N7B39_004718"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3075..3077),aa:Trp,seq:cca)
BASE COUNT          838 a          749 c          777 g          752 t
ORIGIN      
        1 acttgcacaa ataaacagga atcagccact aaaattcacg aattagcggg ctacagcagc
       61 gatatcttat gcacataatg gtgactcaag gagggcttat gtcactgtca attgttcata
      121 cccgcgcagc cctgggagta aatgcgcccc caatcactgt tgaggtacat atcagtaaag
      181 gtttgcccgg cttaacgatg gtgggcttac cagaaacaac ggtaaaagaa gctcgcgatc
      241 gcgtgcgcag cgccattatc aatagcggat atgaatatcc ggcgaaaaaa atcaccatta
      301 accttgctcc agccgatcta ccaaaagaag ggggacgata tgatttacct atcgccattg
      361 cgttgctggc ggcctcagaa cagcttacag ccaataagtt agatgaatat gaattagtcg
      421 gagaactggc gcttacaggc gctctgcgtg gcgttcccgg tgcaatctcc agtgcaactg
      481 aagctattaa gtcgggcaga aaaattatcg tcgcgaaaga taacgaagat gaagtggggc
      541 taattaacgg tgaaggatgc ctggtagccg atcatctgca agctgtctgt gcgtttctgg
      601 aaggtaagca cgctctcgaa cgcccgaaac caactgatgc agtatcccgg gcgctacaac
      661 atgatctcag tgatgttgtc ggtcaggagc aaggaaagcg aggactggaa attaccgccg
      721 ctggcgggca caacctttta ctgattgggc cgccgggaac aggtaaaaca atgctcgcca
      781 gccgtattaa tggtcttttg ccagatttaa gcaatgaaga ggcactggag agcgctgcga
      841 tattaagtct ggtaaatgct gaatcagtac aaaaacaatg gcggcagcgc ccgttccgct
      901 cacctcatca cagtgcgtcg ttaacggcga tggtgggcgg tggtgcaatt ccagggccag
      961 gtgaaatttc gttggcgcat aacggcgtgc tttttcttga tgagctacct gaatttgaac
     1021 ggcgtacact ggatgctttg cgagagccga ttgaatccgg gcagatccat ctttcacgca
     1081 cacgagcaaa aataacctat ccagcccgtt tccagcttgt tgcggcgatg aatcccagcc
     1141 ctaccggaca ttatcaggga aaccataacc gctgcacgcc agaacagaca ttacgttatc
     1201 tcaaccggct ctcggggccc tttctcgacc gcttcgatct ctcactggag atcccattac
     1261 caccccccgg cattttgagt aaaacggtag tgccgggaga aagcagcacc accgttaaac
     1321 aacgtgtaat ggctgccaga gagcgccaat ttaagcggca gaataagttg aacgcctggc
     1381 tggatagtcc ggaaatacgc aaattctgca agcttgagag cgaagatgcg cagtggctgg
     1441 aagaaacgct gatccatctg gggttatcga ttcgtgcctg gcagcggtta ttgaaagttg
     1501 cacgaaccat tgctgatatt gatcagtctg acattatcac acgtcagcat ttgcaggagg
     1561 cagttagcta tcgtgcgatt gaccgtttgc tcatccatct gcaaaaacta ctgacataaa
     1621 aaagggcatt tcgccctttt tattaatcgt cagaatcggt gtagtcttca gcaccttcaa
     1681 cctgcggttt accgccggaa agggtgtgaa aacgttttgg acgcttgata cgcgtcatat
     1741 acttggacca cacgcgttct gcttctgtca ctggctcacg ttcgccacgg catactgcta
     1801 cgaagagttt ctcttcctcg gtaaccggct cgcgtttgcc aagatccaac tcattgaagg
     1861 cataaccatg acgctcaagc agttgtgcct ctttgatggt gaaatcacca tggcgagaga
     1921 atccacgtgg ataatgttta ttgtcgaaat atcgattagt cgtcgtaaag ctttccgcca
     1981 tcctgcacgc tcctaattct ttgaccgagc tagttatggc gcggagtatt agttacgctt
     2041 gacagagtgt aaaacaaaac atttaaatca taacgacaaa taattttgcg gagagcactg
     2101 tggatacgga attgttaaaa actttcctgg aagttagccg aacgcgtcac tttggtcgag
     2161 cggctgaatc gctctatctg acccagtcag cagtgagctt tcgaatcaga caactggaaa
     2221 atcaactggg tgtgaacctt ttcacccgcc acagaaacaa tatccgttta accgctgccg
     2281 gtgaaaaact actgccttat gcagaaacgc tcatgagcac ctggcaggcc gcccgtaagg
     2341 aggtggcgca tacctcacga cataacgagt tttctatcgg tgccagcgcc tcattgtggg
     2401 aatgtatgct taatcagtgg ctgggacgtt tgtatcaaaa tcaggatgcc catacaggct
     2461 tacagttcga agcgcgaatt gcccaacggc agtctctggt aaaacagctg catgaacgcc
     2521 agcttgatct tcttattacc actgaagcgc ccaaaatgga cgaatttagt agtcagttgc
     2581 tggggtattt cactttagcg ctttatacca gtgccccttc taaactaaag ggagatctta
     2641 actatctgcg actggagtgg gggccagatt ttcaacagca tgaggcaggt ttgatcggtg
     2701 ctgacgaagt gcccattctg acaaccagtt ctgctgaact ggcacagcaa cagattgcga
     2761 tgcttaatgg ttgcacctgg ctacccgtca gctgggcgcg taaaaaaggc ggcctgcata
     2821 ccgttgtcga tagcacaaca ctttcacggc cgctttatgc catatggctg caaaatagcg
     2881 ataaaaatac gttgattcgc gatctattga aaattaacgt gctggatgaa gtgtattaat
     2941 ctgaatggct ggcaaggatg ccggtagaag gatttacttc agagagggtt atttcagata
     3001 aaaaaaatcc ttagctttcg ctaaggatga tttctggcag gggcggagag actcgaactc
     3061 ccaacacccg gttttggaga ccggtgctct accaattgaa ctacgcccct aattag
//
LOCUS       DBFIFI010000322         3110 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.324, whole genome shotgun sequence.
ACCESSION   DBFIFI010000322 DBFIFI010000000
VERSION     DBFIFI010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.324"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..142
                     /locus_tag="N7B39_004719"
     CDS             <1..142
                     /locus_tag="N7B39_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HGU2424540.1"
                     /translation="IYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCALSQTYGGNY
                     VS"
     gene            132..1679
                     /gene="fdrA"
                     /locus_tag="N7B39_004720"
     CDS             132..1679
                     /gene="fdrA"
                     /locus_tag="N7B39_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HGU2424541.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRGETPVDEQGLQFARGTKEAALKAVMLSGVKQEHLDLHTLNQPLITDVRA
                     RLKPQQKYIRGLFCGGTLCDETMFAVMEKHSDVYSNIQPDPEFRLKDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            1679..3097
                     /gene="yahG"
                     /locus_tag="N7B39_004721"
     CDS             1679..3097
                     /gene="yahG"
                     /locus_tag="N7B39_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HGU2424542.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQFDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPILRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVAQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAVDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
BASE COUNT          713 a          826 c          939 g          632 t
ORIGIN      
        1 tatttatgac atccctggga acgcaactca ggcaatagaa aaaattaaac atattggctc
       61 cagttccggc tgcgacatgc tgtatggcat ggccgatggt tgtgcgctga gccaaaccta
      121 cggagggaat tatgtcagtt aaaatagtca ttaaaccgaa tacctatttt gattctgtct
      181 cgctgatgtc tatctccacg cgtgcaaata aactcgacgg cgtcgagcag gcatttgtgg
      241 cgatggcgac cgaaatgaac aaaggtgtgc tgaaaaattt aggactgctg acgccggagc
      301 tggagcaggc gaaaaacggc gacctgatga ttgtcatcaa tggtaaatcg ggggcggaca
      361 acgagcagtt gctggtggag attgaagaac tgttcaacac caaagcgcaa agcggctcgc
      421 acgaggcgcg ttacgccact attgccagcg ccaaaaagca tatcccggaa agtaacctgg
      481 cggtgatttc ggtcaacggt ctgtttgccg ctcgcgaagc gcgtcaggcg ctgcaaaacg
      541 atctcaacgt gatgctgttt tccgataacg tctcagttga agatgaactg gcgctcaagc
      601 aactggccca cgaaaaaggg ctgctgatga tggggccaga ctgcggcacg gcgattatca
      661 acggtgcggc gctctgcttt ggtaacgccg tgcgtcgcgg caacatcggt attgttggcg
      721 catccggcac cggcagtcag gagttgagcg tccgcattca tgaatttggc ggcggcgttt
      781 cgcaactgat tggcaccggc gggcgcgacc tgagcgagaa aatcggcggc ctgatgatgc
      841 tcgacgccat cgggatgctg gaaaacgatc cgcaaactga aatcattgcg cttatctcca
      901 aaccgcctgc gcctgcggtg gcccgcaaag tgctggaacg tgcgcgcgcc tgccgcaagc
      961 cggtggtcgt ttgcttcctc ggtcgtggcg aaacgccagt ggatgagcag gggctacagt
     1021 ttgcccgcgg caccaaagag gcagcgctaa aagcggtgat gctctccggc gtgaaacaag
     1081 aacatctcga cctgcatacc ctgaaccagc cgttgattac tgacgtgcgc gctcgcctga
     1141 agccgcagca gaaatacatt cgtggcctgt tctgcggtgg cacgctgtgc gatgaaacga
     1201 tgttcgcggt gatggaaaaa cacagcgatg tctacagcaa catccagccc gatccggaat
     1261 tccgcctgaa ggatatcaac cgcagcatca aacacacctt cctcgacttt ggcgatgacg
     1321 acttcaccaa cggcaagccg cacccgatga ttgaccctac caaccgcatc agccgcttga
     1381 tcgaagaggc gcgcgatcca gaagtggcgg tgatcgtgat ggattttgtg ctcggatttg
     1441 gatcgcatga agatccggtc ggttccacca tcgaggcgat caaagaagcg aaagcgatcg
     1501 ccgctgccga aggacgcgag ttgatcattc tcgcctatgt gctgggtacc gatcttgata
     1561 cgccatcgtt agaacaacaa agccagatgc tgcttgatgc cggcgtgatt ctggcgagca
     1621 gcagcaccaa taccggattg ctggcgcgtg aatttatctg caaaggggag gaagcctgat
     1681 gagccagtca ctgtttagcc aaccattgaa cgttattaac gtcggcatcg ccatgtttag
     1741 cgatgacctg aaaaagcagc atgtagaagt gactcaattc gactggacgc cgccggggca
     1801 gggcaatatg caggtggtgc aggcgctgga taacattgcc gattcgccgc tggcagacaa
     1861 aatcgccgcc gctaaccagc aggcgctgga gcgcattatc cagtcgcatc cggtgctgat
     1921 tggttttgat caggcgatta acgtggtgcc gggcatgacg ccgaaaacca ttcttcacgc
     1981 cgggccgccg atcacctggg aaaaaatgtg cggcgcgatg aaaggcgcgg tcaccggagc
     2041 gctggtgttc gaaggactgg cgaaagatct cgacgaggcg gctgaactgg cggcttccgg
     2101 ggagatcacc ttctcgccgt gtcacgagca cgactgcgtg ggatcgatgg cgggtgtgac
     2161 ctcggcctcg atgtttatgc acatcgtaaa aaacaaaacc tacggcaaca tcgcttatac
     2221 caacatgagc gagcagatgg cgaagatttt gcgtatgggc gctaacgacc agagcgtgat
     2281 tgaccgcctg aactggatgc gtgatgtgca gggaccaata ctgcgcgacg cgatgaaaat
     2341 tatcggcgaa atcgatctgc gcttaatgct ggcgcaggcg ctgcatatgg gcgatgagtg
     2401 ccataaccgc aataacgccg ggacgacgct gctgattcag gcgctgacgc cggggattat
     2461 ccaggcgggc tattccgtcg cgcaacagcg cgaagtgttt gagtttgttg ccagtagcga
     2521 ttacttctcc ggcccgacgt ggatggcgat gtgtaaagcg gcgatggatg cggcgcacgg
     2581 catcgaatac agcaccgtcg ttaccaccat ggcgcgtaac ggcgtcgagt tcggcctgcg
     2641 ggtgagcggc ctgccggggc agtggtttac cggcccagcg cagcaggtca tcggcccgat
     2701 gtttgctggt tataagccgg aagattcggg gctggatatc ggcgacagcg ccatcacaga
     2761 aacctacggt attggcgggt ttgccatggc gaccgcgcct gcaatcgtcg cgctggtggg
     2821 gggcacggtg gaagaggcta tcgatttctc ccgtcagatg cgcgaaatca cccttggcga
     2881 aaaccccaac gtcaccattc cgctgctcgg ttttatgggc gtgccgtcgg cagtcgacat
     2941 cacccgcgtg ggtagcagcg gcattctgcc ggtgatcaac accgccatcg cccataaaga
     3001 tgcgggcgtc ggcatgattg gcgcgggcat tgtgcatcca ccattcgcct gcttcgagaa
     3061 agccattctt ggctggtgcg aacgttacgg cgtttgacct acctgttcat
//
LOCUS       DBFIFI010000323         3090 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.325, whole genome shotgun sequence.
ACCESSION   DBFIFI010000323 DBFIFI010000000
VERSION     DBFIFI010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.325"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..240
                     /locus_tag="N7B39_004722"
     CDS             <1..240
                     /locus_tag="N7B39_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HGU2424543.1"
                     /translation="TPTETDGALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPT
                     TDIRKDRCSKCMRGCELRRNVGNFGGFLSINKLSQ"
     gene            complement(257..577)
                     /locus_tag="N7B39_004723"
     CDS             complement(257..577)
                     /locus_tag="N7B39_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HGU2424544.1"
                     /translation="MAEKQVKDYDKFNLRFPDGMRDAIAERAKRNGRSMNSEIVQILE
                     DALNAENTLGEIADKINSVSVPLNVDALVQLQAQVIAMQKEIQEKFREQNEKLRELLN
                     KKPT"
     gene            685..858
                     /locus_tag="N7B39_004724"
     CDS             685..858
                     /locus_tag="N7B39_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HGU2424545.1"
                     /translation="MQDTLFTERKNIKLNLRLPSRLNEDLRRLAEMDCISLNSAIVRL
                     LAKGVREEVANGR"
     gene            929..1852
                     /locus_tag="N7B39_004725"
     CDS             929..1852
                     /locus_tag="N7B39_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001428824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HGU2424546.1"
                     /translation="MTSLAIADRTINVPFHGTNLFLVGINNEPYVPMKPVVKGMGMVW
                     AAQFVKLKQRFVKGISEIEIPSAGGKQLMTCLAFRKFAAWLSSIQPNKVRPEIRDKVI
                     QYQEECDDVLYEYWTKGHVINPRKAKKALPGKITTEQQEAIKQLVMSRGQSLPKEKQA
                     KAMITMWSSLKSHFGCSYKEISEEQFTEALSLAARVPLEGELIGKQEKSTNELSAKEA
                     NSLVWLWDYANRSQALFRELYPALKQIQSNYSGRCYDYGHEFSYVIGMARDVLINHTR
                     DVDINEPDGPTNLSAWVRLKNKELPPSLHRY"
     gene            1907..2644
                     /locus_tag="N7B39_004726"
     CDS             1907..2644
                     /locus_tag="N7B39_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000967278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HGU2424547.1"
                     /translation="MLYSNILAHARRCAPAESCGFVVRTPEGERYIPCVNISAEPEAY
                     FRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALSWWLVCRGDIHKFR
                     CVPHLTGRRFEHGVTDCYTLFRDAYHLAGIDMPDFEREDDWWRNGQNLYLDNMAVTGF
                     YRVPLSSAQAGDILLCCFGASVANHAAIYCGNGELLHHLPEQLSKRERYSEKWQRRTH
                     SVWRHRHWHASAFTGICNDLAAASACM"
     gene            2641..>3090
                     /locus_tag="N7B39_004727"
     CDS             2641..>3090
                     /locus_tag="N7B39_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HGU2424548.1"
                     /translation="MNTAAEAIRALSLQVPGFRRQMNEGWYQIRIRGEDTAPEAVYAR
                     LHEPLGEGAVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGATMALWGAALSAGGLT
                     ATTMLFSLGASMILGGVAQMLAPKAKTPEYRATDNGKQNTYFSSLDNM"
BASE COUNT          756 a          737 c          839 g          758 t
ORIGIN      
        1 acaccgaccg agacggacgg ggcgctgttt cccggtcgca tcatgctggc gaacacctgt
       61 atgtgggatt accggggaga tgaatgcggg tataacggtc ctgcggtggc ggatgagttc
      121 gacaacccca ccacggatat ccgtaaggac agatgcagca agtgcatgcg cgggtgtgaa
      181 ctgcgcagga atgtcggcaa ttttggcggt ttcctttcca ttaataaact ttcgcagtaa
      241 tggattatgc ccaccgtcag gtgggttttt tatttagtag ttctctcaac ttttcgttct
      301 gctctctgaa cttttcctgt atttcttttt gcatggcgat aacctgggct tgaagttgaa
      361 ctagcgcatc aacatttagc ggaaccgaga cgctgttaat tttatctgct atttccccga
      421 gtgtattttc tgcattcaag gcatcttcca gtatctgaac aatctctgag ttcatagagc
      481 gcccgtttcg tttggctcgt tcagctatag catctcgcat tccgtcagga aaacggaggt
      541 tgaacttgtc gtagtctttt acttgttttt cggccattgc aaatctctca aaaaaaatca
      601 tggtgccata ttgccatacg atttcaatgg tggcattatg gccttcatgg cgtcactttg
      661 gcaccaaata aaggagatta gataatgcaa gatacacttt tcactgagcg caaaaatatc
      721 aaactcaacc ttcgccttcc atcacggctg aatgaagacc ttcgccgcct ggcggaaatg
      781 gactgtatat ctctgaactc tgcaattgtt cgtttgctgg caaaaggtgt tagggaagag
      841 gtggcgaatg gtcgctaaaa acagcgaagc ctcaatggct gcaaccattg aggcttctaa
      901 attaccagtt aaccacgaga aaactgatat gactagttta gcaattgcag atcgcacaat
      961 caatgttcca ttccacggaa caaatctctt tttggttgga attaacaatg agccttatgt
     1021 tcctatgaag cctgttgtta aaggtatggg gatggtttgg gctgctcaat ttgttaagtt
     1081 aaaacagagg tttgtcaaag gtatttcgga aatcgaaata ccttctgctg gcggtaaaca
     1141 gttaatgaca tgtcttgcct ttcgtaaatt tgcggcttgg ctttcaagca ttcaaccaaa
     1201 caaagtccgc cctgaaatcc gcgacaaggt aatccagtat caggaagagt gtgacgatgt
     1261 gctctacgag tactggacta aaggccatgt gattaacccg cgcaaagcta aaaaggcatt
     1321 gccggggaaa atcaccactg aacagcagga agccattaaa caactcgtca tgagtcgcgg
     1381 tcagtctctg ccaaaagaaa aacaggcgaa ggcgatgatc accatgtggt cgtcactgaa
     1441 atctcatttt gggtgttcat acaaagaaat cagcgaggag cagtttaccg aagctctgtc
     1501 acttgctgct cgcgttccgc ttgaaggaga gttaatcggc aaacaagaga agagcaccaa
     1561 cgagctttcc gcaaaagaag caaacagcct tgtatggtta tgggattatg ctaaccgctc
     1621 acaggcatta ttccgcgaac tgtatccggc gctaaaacaa attcaatcga actattccgg
     1681 cagatgctac gactacggtc atgagttttc gtatgttatc ggaatggcga gagatgtttt
     1741 aatcaaccac acacgagatg ttgatattaa tgagccagac ggaccaacga acctttccgc
     1801 atgggtaagg cttaagaaca aagagttgcc gccttcactg catcgctact gacaattgac
     1861 aacttaacaa acccagcttc ggctgggttt tttatcagga gttctcatgc tctatagcaa
     1921 tatattggcg cacgcccggc gatgtgcgcc agcggagtcg tgcggcttcg tggtgagaac
     1981 gccggagggg gagcggtata tcccttgtgt gaatatctct gcagagccgg aggcgtattt
     2041 tcgtattgca ccggaagact ggctgcgggc agagatgcag ggggagattg tggcactggt
     2101 ccacagtcat cccggtggtc tgccctggct gagcgaggcc gaccggcggc tgcagataaa
     2161 aagtgcactg tcctggtggc tggtctgccg gggggacatt cataaattcc gctgtgtgcc
     2221 acatctgaca ggacggcgct ttgagcacgg ggtgacggac tgttacacgc tgttccggga
     2281 tgcataccat ctggcgggaa ttgatatgcc ggattttgag cgtgaggatg actggtggcg
     2341 taacggtcag aacctttacc tggacaatat ggcggtcacc ggcttttacc gggtgcccct
     2401 gtcctctgca caggcgggcg atatcctgct gtgctgcttt ggcgcatcgg tggccaatca
     2461 tgccgccata tactgcggca acggtgaact gcttcaccat ctgcctgaac aactgagtaa
     2521 acgggagagg tattccgaaa aatggcaacg acgaacgcat tctgtctggc gtcaccgcca
     2581 ctggcacgca tctgccttca cggggatttg caacgatttg gccgccgcct cagcctgtat
     2641 gtgaacacgg cagcggaagc catccgggcg ctgtcgttac aggtgccggg attccgccgt
     2701 cagatgaacg aaggctggta ccagatacgt attcgcggtg aggacacggc accggaggcg
     2761 gtgtacgccc gtcttcacga acctctgggt gagggggcgg tcatccatat tgtgccgcga
     2821 ctggccggag ccggaaaggg cggactgcag attgtgctgg gggcagcagc catcgtgggc
     2881 tctttcttca ccgccggcgc aacgatggcg ttgtggggcg cagccctgag tgccggaggg
     2941 ctgactgcca ccacgatgct gttctcactg ggtgccagca tgatactggg cggtgtggcc
     3001 cagatgctgg caccgaaggc aaaaacaccg gagtacaggg cgacggataa cggtaaacag
     3061 aacacgtatt tttcgtcact ggataacatg
//
LOCUS       DBFIFI010000324         3083 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.326, whole genome shotgun sequence.
ACCESSION   DBFIFI010000324 DBFIFI010000000
VERSION     DBFIFI010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.326"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..1250)
                     /gene="escN"
                     /locus_tag="N7B39_004728"
     CDS             complement(<1..1250)
                     /gene="escN"
                     /locus_tag="N7B39_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ATPase EscN"
                     /protein_id="HGU2424549.1"
                     /translation="MISEHDSVLEKYPRIQKVLNSTVPALSLNSSTRYEGKIINIGGT
                     IIKARLPKARIGAFYKIEPSQRLAEVIAIDEDEVFLLPFEHVSGMYCGQWLSYQGDEF
                     KIRVGDALLGRLVDGIGRPMESNIAAPYLPFERSLYAEPPDPLLRQVIDQPFILGVRA
                     IDGLLTCGIGQRIGIFAGSGVGKSTLLGMICNGASADIIVLALIGERGREVNEFLALL
                     PQSTLSKCVLVVTTSDRPALERMKAAFTATTIAEYFRDQGKNVLLMMDSVTRYARAAR
                     DVGLASGEPDVRGGFPPSVFSSLPKLLERAGPAPKGSITAIYTVLLESDNVNDPIGDE
                     VRSILDGHIVLTRELAEENHFPAIDIGLSASRVMHNVVTSEHLRAAAECKKLIATYKN
                     VELLIRIGEYTMGQDPEADKAIKN"
     gene            complement(1234..>3083)
                     /gene="escV"
                     /locus_tag="N7B39_004729"
     CDS             complement(1234..>3083)
                     /gene="escV"
                     /locus_tag="N7B39_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscV"
                     /protein_id="HGU2424550.1"
                     /translation="KNPLELTSFPTILLITTLMRLSLSVSTTRLILLHHDAGDIIYSF
                     GNFVVGGNIVVGLVIFTIITIVQFMVITKGAERVAEVSARFSLDGMPGKQMSIDGDMR
                     AGVIDPLEAKVLRSRVQKESQFYGSMDGAMKFVKGDAIAGIIIVLVNLFGGVLIGMWQ
                     FDMPFSAALSLFSVLSVGDALVAQIPALIISVTAGVVVTRVPGESEKEENLAGDIVQQ
                     VSVNSRPFLISAALMLVMAIIPGFPALVFLFLAVCLLGIAWKLQKKRTFGTGNNKDAM
                     GADLSNSQNISPGAEPLILNLSSNIYSSDITQQIEVMRWNFFEESGIPLPKIIVNPVK
                     NNDSAIEFLLYQESIYKDTLIDDTVYFEAGHAEISFEFVQEKLSTNSIVYKTNKTNQQ
                     LAHLTGMDVYATTNDKITFLLKKLVLSNAKEFIGVQETRYLMDIMERKYNELVKELQR
                     QLGLSKIVDILQRLVEENVSIRDLRTIFETLIFWSTKEKDVVILCEYVRIALRRHILG
                     RYSVSGTLLNVWLIGSDIENELRESIRQTSSGSYLNISPERTEQIIGFLKNIMNPTGN
                     GVILTALDIRRYVKKMIEGSFPSVPVLSFQEVGNNIELKVLGTVNDFRA"
BASE COUNT          937 a          746 c          580 g          820 t
ORIGIN      
        1 ctatttttta ttgccttatc tgcttcagga tcttgcccca tcgtgtactc accaatacga
       61 ataagcagct caacattttt ataagttgca ataagctttt tgcattctgc cgccgcacgc
      121 aaatgctccg atgtaacaac gttatgcata acacgactgg cacttaaacc aatatcaatt
      181 gcagggaaat ggttttcctc tgcaagttct cgggtaagta cgatgtgccc atcaagaata
      241 gagcggactt catcgccaat aggatcatta acattatcgc tttctaacaa caccgtataa
      301 atcgcggtta ttgaaccttt tggcgcaggc cccgctcgct ctaataattt gggtaacgaa
      361 gaaaaaacac tcggagggaa tccccccctt acatcaggtt ctcccgatgc aagcccaaca
      421 tcacgtgcgg ctcgggcata acgagttaca gaatccatca ttaataatac atttttacct
      481 tgatcgcgga agtactctgc aatagtcgtc gccgtaaatg cggctttcat cctttccagc
      541 gcggggcggt ctgacgttgt gacgaccagt acacatttag aaagcgtgga ttgaggtaag
      601 agcgcgagga attcatttac ttcgcgacca cgttcgccga taagagcaag gacaataata
      661 tctgcggatg cgccattaca aatcatcccc aaaagcgtac ttttaccaac gcctgaacca
      721 gcaaaaatac cgatacgctg cccaataccg caagttagta gcccatcaat tgccctgacg
      781 ccaagtataa atggctgatc aataacctgc cttaataaag gatctggtgg ttcagcatac
      841 aagctgcgtt caaacggaag atagggggca gcaatattac tctccattgg tctgcctatg
      901 ccatcaacaa gccgcccaag tagtgcgtct ccaacacgaa tcttgaactc atctccctga
      961 taacttaacc actgaccaca gtacatgccg gaaacatgct caaaaggcaa caagaaaacc
     1021 tcatcttcat caatagcgat cacctcggct aaacgttgac tgggttctat cttataaaag
     1081 gcgccaatac gcgcttttgg taaacgcgcc ttaataatcg tcccgccgat attgataatc
     1141 ttgccttcat atctggtaga cgaatttaat gataatgccg gcacagtgct attgagcact
     1201 ttctgaatac gtgggtattt ttccaataca gaatcatgct ctgaaatcat ttaccgttcc
     1261 taatacttta agttcgatat tattcccaac ctcctgaaaa gagagcacgg ggactgacgg
     1321 gaacgaacct tcaatcattt tcttcacata gcgcctgata tctaaagcgg tcagaatgac
     1381 gccgttcccc gttggattca tgatattttt taagaagcca attatctgct cagttcgctc
     1441 cggtgagata ttcagatacg aacctgatga cgtttgtctg atcgactctc gtagctcatt
     1501 ttcaatatca gagccaataa gccaaacgtt cagaagtgta ccgctaacgc tatagcgacc
     1561 taaaatatgc cgacgcaggg caatacgaac gtattcgcac aagataacca catccttttc
     1621 ttttgttgac caaaaaataa gcgtctcaaa gatagttctc aggtctctaa ttgagacatt
     1681 ttcctctacg agacgttgta ggatgtcaac aattttgctc aaaccaagct ggcgctgcag
     1741 ctctttcaca agctcgttat attttctctc catgatgtcc atcaaataac gcgtttcttg
     1801 tacgccgatg aactctttgg cattagacaa tacaagtttc tttagcaaaa acgttatctt
     1861 atcatttgtt gttgcataaa catccatacc tgtaaggtga gcgagctgtt gattagtctt
     1921 attcgtctta tatacgatag agttagtcga aagcttttcc tggacaaatt cgaatgatat
     1981 ctctgcatgc ccagcctcaa aatagacagt atcatctata agagtatctt tgtatattga
     2041 ctcttgatag agcaaaaatt ctattgcgct atcattattt ttaaccggat taacaataat
     2101 cttaggcaat ggaattccac tttcctcaaa gaaattccaa cgcatgacct caatttgctg
     2161 tgtaatgtct gaactataaa tattactact taagtttaaa atcaatggct cagcgcccgg
     2221 tgagatgttt tggctattag acaaatcagc tcccatagca tccttattat tgccagttcc
     2281 aaatgttctt ttcttctgta atttccaggc tatccccaac aggcaaaccg ccagaaataa
     2341 gaagaccaat gcaggaaagc ccggaataat cgccattacg agcattagtg cggcactgat
     2401 taaaaaaggg cgactattaa cagaaacctg ttgaacaata tcacctgcaa ggttttcttc
     2461 tttttcgctt tcaccgggca cacgagtaac aaccacgcct gcggtgacag aaataataag
     2521 cgcagggatc tgggcaacca aagcatcacc gacagacaat acagaaaaca ggctaagtgc
     2581 cgcactaaat ggcatgtcaa attgccacat accaatgagc acgccaccaa aaaggttaac
     2641 taaaacaata atgataccag caatagcgtc cccttttaca aacttcatcg ccccgtccat
     2701 tgaaccataa aactggcttt ccttttgcac tcgggagcga agaacttttg cctcaagcgg
     2761 gtcaataacg cctgcgcgca tatcaccatc aatactcatt tgctttcctg gcataccatc
     2821 aagggaaaaa cgagcactca cttcagcaac acgctcggcc ccttttgtaa taaccataaa
     2881 ttgaacaatg gtgataatag taaaaataac caggccaaca acaatattac cgccaacaac
     2941 aaagttaccg aaggagtaaa taatgtcacc cgcatcgtga tgcagcagaa taagtctggt
     3001 tgtactaaca ctgagcgaca ggcgcatcaa cgtcgtaatc aacaggattg tggggaaaga
     3061 agttagttca agaggatttt tta
//
LOCUS       DBFIFI010000325         3013 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.327, whole genome shotgun sequence.
ACCESSION   DBFIFI010000325 DBFIFI010000000
VERSION     DBFIFI010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.327"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..557
                     /gene="espA"
                     /locus_tag="N7B39_004730"
     CDS             <1..557
                     /gene="espA"
                     /locus_tag="N7B39_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     filament protein EspA"
                     /protein_id="HGU2424551.1"
                     /translation="VVNVSASSSTSTIYDLGNMSKDEVVKLFEELGVFQAAILMFSYM
                     YQAQSNLSIAKFADMNEASKASTTAQKMANLVDAKIADVQSSTDKNAKAKLPQDVIDY
                     INDPRNDISVTGIRDLSGDLSAGDLQTVKAAISAKANNLTTVVNNSQLEIQQMSNTLN
                     LLTSARSDVQSLQYRTISAISLGK"
     gene            570..1694
                     /gene="espD"
                     /locus_tag="N7B39_004731"
     CDS             570..1694
                     /gene="espD"
                     /locus_tag="N7B39_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     pore-forming subunit EspD"
                     /protein_id="HGU2424552.1"
                     /translation="MLNVNNDTLSVTSGVNTASGTSGITQSETGLSLDLQLVKSMNSS
                     AGWTESSPLPTPPAGHSLVTPSAAEDVLSKLFGGISGEVTSRTEEAEPQRTSYPYLSQ
                     VNTVDPQQMMMMVTLLSLDTSAQKVSSLKNSNEIYMDGQTKALENKTQEYKKQLEEQQ
                     KAEEKSQKSKIVGQVFGWLGVALTAVAAVFNPALWAVVAIGATAMALQTAVDVMGENA
                     PQGLKTAAQVFGGISMAASILTAGVGGVSSLLSKFGNVANKIGSSVVKVVEKAAEALV
                     KNVFAKISTVAEGVTNGIRSAGTTALNNEAAQLQMLSQLAAFAVQNLTRQSESLGESA
                     KLELDKAASELQNQASYLQSVSQLMSDSARVNSRIVSGRI"
     gene            1715..2653
                     /gene="espB"
                     /locus_tag="N7B39_004732"
     CDS             1715..2653
                     /gene="espB"
                     /locus_tag="N7B39_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     pore-forming subunit EspB"
                     /protein_id="HGU2424553.1"
                     /translation="MNTIDNTQVTMVNSASESTTGASSAVAASALSIDSSLLTDGKVD
                     ICKLMLEIQKLLGKMVTLLQDYQQKQLAQSYQIQQAVFESQNKAIEEKKAAATAALVG
                     GIISSALGILGSFAAMNNAAKGAGEIAEKASSASSKAAGAASEVANKALVKATESVAD
                     VAEEASSAMQKAMATTTKAASRASGVADDVAKASDFAEDLADAAEKTSRINKLLNSVD
                     KLTNTTAFVAVTSLAEGTKTLPTTISESVKSTHEVNEQRAKSLENFQQGNLELYKQDV
                     RRTQDDITTRLRDITSAVRDLLEVQNRMGQSGRLAG"
     gene            2660..>3013
                     /gene="cesD2"
                     /locus_tag="N7B39_004733"
     CDS             2660..>3013
                     /gene="cesD2"
                     /locus_tag="N7B39_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LcrR family type III secretion system chaperone
                     CesD2"
                     /protein_id="HGU2424554.1"
                     /translation="MVDTFNDEVFNYYLEQKGYTIQKEFLCGSAFFIGWRIETPFFSL
                     AYRLDEQELILCSFEARNQTGLNGPVLSLTHLLEELYHHFSGIKKISAMKSKIGSDSE
                     RQKREELFNYFIRKGA"
BASE COUNT          865 a          598 c          752 g          798 t
ORIGIN      
        1 ccgttgttaa tgtgagtgcg agttcttcga catcgacgat ctatgactta ggtaatatgt
       61 cgaaggatga ggtggttaag ctatttgagg aactcggtgt ttttcaggct gcgattctca
      121 tgttttctta tatgtatcag gcacaaagta atctgtcgat tgcaaagttt gctgatatga
      181 atgaggcatc taaagcgtca accacggcac aaaagatggc taatcttgtg gatgccaaaa
      241 ttgctgatgt tcagagtagc actgataaga atgcgaaagc caaacttcct caagacgtga
      301 ttgactatat aaacgatcca cgtaatgaca taagtgtaac tggtattcgt gatcttagtg
      361 gtgatttaag cgctggtgat ctgcaaacag tgaaggcggc tatttcagct aaagcgaata
      421 acctgacaac ggtagtgaat aatagccagc tcgaaattca gcaaatgtcg aatacattaa
      481 atctcttaac gagtgcacgt tctgatgtgc aatctctaca atatagaact atttcagcaa
      541 tatcccttgg taaataaccg gagataacta tgcttaacgt aaataacgat accctgtctg
      601 taacgtctgg ggttaatacc gcctcgggta cttctggtat tactcaatct gaaacgggtt
      661 tatcgctgga tttacaactg gttaaatcca tgaactcgtc agcaggctgg acagaaagta
      721 gccctttacc gacgccgccg gcaggtcact cattagtgac gccctctgct gctgaggatg
      781 tccttagtaa attgtttggt ggtattagtg gtgaggttac aagtcgcact gaggaggcag
      841 agccacagcg cacaagctat ccctatctct ctcaggtgaa taccgttgac cctcagcaaa
      901 tgatgatgat ggtcactctg ttatccctgg atacttccgc gcagaaagtc tcgagtctga
      961 aaaactctaa cgagatttat atggatgggc aaactaaagc gctggagaat aaaacgcagg
     1021 agtataaaaa acagctcgaa gaacaacaga aagccgaaga gaaatcacaa aaaagtaaaa
     1081 ttgttggcca ggtctttggt tggttgggcg tcgcattaac agccgttgcc gctgttttta
     1141 acccagcact ctgggctgtt gttgccattg gtgcaacagc aatggcactg caaacggcag
     1201 tcgatgtaat gggggaaaat gcccctcagg gattaaagac tgcagcacag gtctttggcg
     1261 gaatatctat ggccgcaagc attctgacag ccggcgttgg cggggtgtct tcactgttat
     1321 ctaaatttgg taatgttgct aacaaaattg gctcaagcgt tgtaaaagtc gttgagaagg
     1381 cggcagaagc gctggttaaa aacgtttttg caaaaatttc gacagtggct gagggcgtta
     1441 cgaacggtat tcgttctgcc gggacaactg cgttgaataa tgaggctgcg caactccaaa
     1501 tgttgtctca gttagctgct ttcgcggtgc aaaacttaac tcgacagagt gaaagcttag
     1561 gtgagagtgc gaagctcgag ctggataaag cggcaagcga gttacaaaat caggcgagct
     1621 atttacaaag tgtttctcaa ctgatgtccg attcagcacg ggtaaatagt cgtattgtta
     1681 gtggccgaat ttaattatta aagagaattt tattatgaat actattgata atactcaagt
     1741 aacgatggtt aattccgctt cggagagtac gaccggcgct tccagtgcag ttgccgcatc
     1801 tgctttatca attgattcat ctctgcttac tgatggtaag gttgatattt gtaagctgat
     1861 gctggaaatt caaaaactcc tcggcaagat ggtgactcta ttgcaggatt accaacaaaa
     1921 acaattggcg caaagctatc agattcagca ggccgttttt gagagccaga ataaagctat
     1981 tgaggaaaaa aaagccgcgg caaccgctgc tttggttggc gggattattt catcagcatt
     2041 ggggatctta ggttcttttg cagcaatgaa caacgcggct aaaggggctg gtgagattgc
     2101 tgaaaaagca agctctgcat cttcaaaggc tgctggtgcg gcttctgagg ttgcaaataa
     2161 agctctggtc aaggctacgg aaagtgttgc tgatgtcgca gaggaggcat ccagtgcgat
     2221 gcagaaagcg atggccacaa caacgaaagc agccagccgt gcatctggcg ttgcagatga
     2281 tgttgcgaaa gcctctgact ttgctgaaga tcttgcagac gccgccgaga agacaagcag
     2341 aatcaataag ttgttgaatt ccgtagataa actgaccaat accacagcat ttgttgccgt
     2401 gaccagtctt gctgaaggta cgaaaacgtt gccaacaaca atatctgagt ccgtcaaatc
     2461 gactcatgag gttaatgaac aacgtgcgaa gtcgctggaa aacttccagc aggggaatct
     2521 ggagctgtat aaacaagacg ttcgcagaac gcaggatgat atcacgactc gtctgcgtga
     2581 tataacgtcc gctgtccgcg atctccttga ggtccagaat cgtatggggc aatcgggtcg
     2641 cttagctggg taattgatca tggtcgatac gtttaatgat gaagtgttta attactatct
     2701 tgaacaaaaa gggtacacaa tacagaagga gtttctttgt ggcagtgcct tttttatcgg
     2761 atggcggatt gagacacctt ttttttcatt agcgtacaga ctggatgaac aagaactgat
     2821 tttgtgctct tttgaagcac gtaaccaaac agggcttaac ggccctgttt tatcgctgac
     2881 tcatttgctc gaagagttgt atcaccattt ttcgggtatt aagaaaatca gcgcgatgaa
     2941 atctaagatt ggttcagatt cagaacgtca aaagcgcgaa gagttgttta attacttcat
     3001 cagaaagggt gcc
//
LOCUS       DBFIFI010000326         2988 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.328, whole genome shotgun sequence.
ACCESSION   DBFIFI010000326 DBFIFI010000000
VERSION     DBFIFI010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.328"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            314..1546
                     /locus_tag="N7B39_004734"
     CDS             314..1546
                     /locus_tag="N7B39_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HGU2424555.1"
                     /translation="MSDTLLTEKILTGENVLRAAIARIEWIFETFPSVCLSFSGGKDS
                     TVLFHLVAEVARRRKRHFSVLFIDWEAQYRCTIEHIQKMREMYHDVTETFYWVALPLT
                     TVNGVSQFQPEWICWEPGVTWVRQPPEEAITDMTYFPFFRYAMTFEEFVPAFSSWFAG
                     NRCGVAVLTGVRADESLNRFMGLVSQRKLRYADDKPWTTASPEGFYYTMYPLYDWKAR
                     DIWIYNARACAIYNPLYDLMYRADVPLRNMRVCEPFGPEQRKGLWLYHVLEPETWARM
                     CERVSGAASGALYANESGAYFALRKRISKPAHHTWRSYAMFLLDVMPERTAEHYRNKI
                     AVYLRWYQTRGFPDDIPDEQENDLGSRDIPSWRRICKTLIKNDFWCRPLSFSPNKPRH
                     YERYLQRMKERRKEWGIL"
     gene            1531..2169
                     /locus_tag="N7B39_004735"
     CDS             1531..2169
                     /locus_tag="N7B39_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HGU2424556.1"
                     /translation="MGDSVTPEIIGNMIRQYFSQERPEEETIQALNHLRRVLHEVSPF
                     AHEPVDCVLWVKADEVVANDYNPNVMAPGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQVLGREAGTGKRLKGWLPVVCINPERKGQASRIAATIRHNRARGKHQITSMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSPAWTVR"
     gene            2248..2517
                     /locus_tag="N7B39_004736"
     CDS             2248..2517
                     /locus_tag="N7B39_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HGU2424557.1"
                     /translation="MVAQKLEAAGCWRRASDRWLFVMGNVECTEAQREWLLLRRNYCL
                     AQISSPPLPETLDISEVAKAADATLRRMGIATPSGEVFRKGTPVC"
     gene            2538..>2988
                     /locus_tag="N7B39_004737"
     CDS             2538..>2988
                     /locus_tag="N7B39_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424558.1"
                     /translation="MMKQGMANSISMTWQLLCDEHGFTREHYNQLKKFSPETLREIIA
                     EIASCHPSTSVLLRNKWLTPPEDILEQITREYERRIQNCPPFRSEKEAESWLNELSFA
                     VIAPLYRVAPEQTEQVESFVLQLIAEQERVWELILTNDGYSWHCALYD"
BASE COUNT          773 a          627 c          825 g          763 t
ORIGIN      
        1 cttagtgaat aaactttatc agggggtaat tctattacag tgaatacttc ctgcaaaatg
       61 atatttgaat tgttcaccat ctgcatcata ttttcttatt tacaagttga aaggatagta
      121 tatgttgaaa atgcatggat tttccaggtg tgttaccagt aagaagcagg tggataaagg
      181 atgtgataac aaatccaaca accaggttaa tatctgaaga tttttgttgt tattatttat
      241 ccccaaagcg gtgagttatt aatgaagttt gcctgaatgt ataaacagaa ttattcgaca
      301 taaagccagg aaaatgagtg atacgctttt aaccgaaaaa atccttaccg gagaaaatgt
      361 tttgcgtgct gcaatcgccc gcattgagtg gatttttgaa acatttccgt cggtatgtct
      421 gtctttttca ggtggaaagg attccaccgt gcttttccat cttgtggcag aggtggcccg
      481 caggagaaaa cgtcatttct ctgttctgtt cattgactgg gaggcccagt atcggtgcac
      541 cattgaacat attcagaaga tgcgggaaat gtaccatgat gtgacggaaa ccttttactg
      601 ggtggcactt cccctgacca cggtaaacgg cgtctcgcag tttcagccgg agtggatatg
      661 ctgggagcca ggagtgacct gggttcgcca gccaccggaa gaggccatta cggatatgac
      721 atattttccc tttttccggt atgccatgac gtttgaagaa tttgttccgg cattttcttc
      781 ctggtttgcc ggtaaccggt gtggagtggc agtgctgacc ggtgttcgtg cggatgaatc
      841 cctcaatcgc tttatggggc tggtgtctca gcgcaaactg agatatgccg atgataagcc
      901 ctggaccacg gcatcgcctg aagggtttta ttacaccatg tatccgttgt atgactggaa
      961 agcccgcgat atctggatat ataacgccag agcttgtgcc atttacaatc ctctgtatga
     1021 cctgatgtac cgtgccgacg tgccgttacg caacatgcgt gtctgtgaac cttttggtcc
     1081 tgaacaacgt aaggggctgt ggctttacca tgttctggag ccggaaacct gggccaggat
     1141 gtgtgagcgg gtgtcaggcg ctgccagcgg ggcgctttat gccaatgaaa gcggtgccta
     1201 ttttgccctg cgtaaacgta tatcaaaacc agcccatcat acctggcgca gctatgcgat
     1261 gttcctgctg gatgtgatgc cggaaagaac ggcagaacat taccgtaata aaattgctgt
     1321 ctacctgcgc tggtatcaga cgcggggctt cccggatgac atcccggatg aacaggagaa
     1381 tgacctgggg agccgggata ttccgtcctg gcggcgtatc tgtaagacac ttataaagaa
     1441 tgatttctgg tgccggcccc tctccttcag tccgaacaaa ccccggcact atgaacgtta
     1501 tctgcagcgt atgaaagaaa ggaggaagga atgggggatt ctgtgacacc ggagataatt
     1561 ggcaacatga ttcgtcagta cttcagccag gaacgaccag aagaagagac catacaggcc
     1621 cttaatcacc tgcgcagagt gcttcatgaa gtcagtcctt ttgcacatga gccagtggac
     1681 tgtgtgctgt gggtaaaagc agatgaggtg gtggccaatg attacaatcc taatgtcatg
     1741 gcgccgggtg agaagaaact gctgaagcag tcactggaga aagatggttt cacacaaccg
     1801 gtggtggtgt ctgaggaaaa gaaccattac ctggtggtgg atggttttca tcgccaggtg
     1861 ctgggcaggg aagcaggtac gggaaaacgt ctgaagggct ggctgccggt ggtctgtatt
     1921 aatccggaga gaaagggaca ggcctcacgt attgctgcga ccatccgaca taaccgggcc
     1981 aggggcaaac atcagataac ctcaatgtct gatattgtca gggacctgtc gcgcctgggc
     2041 tggacgaatg aacgcattgg tacggagctg ggtatggacc aggatgaagt tctcagattg
     2101 aaacagatta gcgggctgac ggagctgttt caggaagagg atttcagccc ggcatggacg
     2161 gttcgctgag accgtcagat atgtatatta tttcagtgag ttaatgtttt gagcaggaaa
     2221 aagagtaccc cggaagttgt ggaagacatg gttgcgcaga aactggaggc tgctggttgc
     2281 tggcgcagag cttctgaccg ctggcttttt gttatgggaa atgttgaatg tacagaagca
     2341 caacgggagt ggttgttgtt gcgccggaat tattgtctgg cgcagatatc gtccccgccg
     2401 ctacctgaaa cgctggatat cagcgaggtg gcgaaagctg cagatgccac gctgaggcga
     2461 atggggattg ccactccgtc cggggaggta ttcagaaaag gtacaccggt ctgttgatat
     2521 tcaggtaaca ggtattaatg atgaagcaag gaatggcaaa cagtatatca atgacatggc
     2581 aacttttatg cgatgaacat ggttttaccc gggagcacta caatcagctg aagaagttca
     2641 gcccggagac attacgtgaa attattgctg aaatagccag ctgtcatcca tcaaccagtg
     2701 tattgctcag gaataagtgg ctgacaccgc cggaggatat tctggagcag attacccgtg
     2761 aatacgaacg aagaatacaa aactgtccac cgttcaggag cgagaaggaa gcggaatcct
     2821 ggttaaatga actatctttt gctgtgattg ctccgctata ccgtgttgcc cccgaacaaa
     2881 cagaacaggt tgagtccttt gttctgcagc ttattgccga gcaggagcgg gtttgggagc
     2941 ttattttaac caatgatggt tattcatggc attgcgcact ttatgata
//
LOCUS       DBFIFI010000327         2974 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.329, whole genome shotgun sequence.
ACCESSION   DBFIFI010000327 DBFIFI010000000
VERSION     DBFIFI010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.329"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(110..1588)
                     /locus_tag="N7B39_004738"
     CDS             complement(110..1588)
                     /locus_tag="N7B39_004738"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HGU2424559.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSED
                     AAVMEKFNIPRHMLFDVQMPGTVLGQITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASCVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFIPIESNAKRY
                     DAMNKGIFKALTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
     gene            complement(1615..2892)
                     /locus_tag="N7B39_004739"
     CDS             complement(1615..2892)
                     /locus_tag="N7B39_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HGU2424560.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSTSLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSVLGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTQPAAEAYRNMWL
                     MGMAALAMVIVFTFLLFQKIRTADSAPAMASSK"
BASE COUNT          777 a          786 c          680 g          731 t
ORIGIN      
        1 ccggacttgt tatcattttt taagttgtcg aacttatgtt ccccgcgcca gcggggataa
       61 ccattcaggt atgttcaggc cgggcagttt cccgcccggt cttcctcact tacgcattcg
      121 accagctctg gatcgaatcc gcattcccca attccccatg catcacttca tacgattttt
      181 tcaggatcac atcagtatgt ttggttaggg ctttgaaaat acctttattc atcgcgtcgt
      241 agcgtttggc attgctctca atcggtataa agatatcttt cacgcgaacc attttgtcga
      301 cagccgttgc gtaatccggg tatagcccca gacctaccgc cgtattgatc gctgccccca
      361 gacttgcaca accgttaatg gcgttacgtc gtgccggaag gttgaacacg tcggcaaaaa
      421 tctgcataaa cagatcgctg ttcgaaccgc caccagtaat gatcacatgc ttcgcaaagt
      481 gattcatttc attacacata ttgtcgtaat tgttcttcag cgtcagcgcc acgctttcca
      541 gtatcgaacg atatatccat gcgtaatcca tgctggaatc aaagccgatc ataatcccgc
      601 gtttgtacgg ttcccacgga ttggtcagcc agtccagcac cgtcatcagc ccattacagc
      661 caggtggcac acaagaagct tttttgttga gtaaatcttc cggtgaaaga tcctgcgccc
      721 tggcatcctg aattaacgac tcgcctaaca tgtcgcgcag ccagctcacc gtccacatgc
      781 ctttgcgaat accgtaacct tcatacagca atgtttgcgg aatagaagac ataatcggcc
      841 agtacgccac cggatctttc ggcaaggctt tgccgttcat catcagcgcg atataagtac
      901 cgagtgaaat caccgccgtt tcatcatcca gtaatccggc tcccagagct tctaccggtt
      961 tatcactggt ggtacaaaca accggcagcc ctgccgggaa atgtgtcgcc agcgcggctt
     1021 gtggtgtgat ctgtccaaga acagtgcctg gcatttgcac atcaaacagc atatgacggg
     1081 ggatattaaa cttctccatt accgcagcat cttcgctcca tgcccagctc ttataatcca
     1141 ccggccactg accaaaatag ttggcgatat tgtctttaaa ctcgccggtt aagcgatgcg
     1201 tcagataacc cgaaaaagag gtgacatagg ccacgtcagg attagtgtgt tcgtagggac
     1261 gcgttacgcg tgcatcctgc cagctaatca acggcgcagc aggcgtgcca tcggctttca
     1321 ataacgcacg gcagcaacgg atggatccca gaccaatacc gacaatatct tctttattcc
     1381 cggcaaactg actcatcaaa tcgtgaccgg caaaacataa tgatgcccat aaatcgtcgt
     1441 caggatgttc tgcggtatcg gcatctggcg tgtgcatcgg ctgcaataaa cctttgcctt
     1501 cgcaaaccac gttaccttcc agatcgtaca tcaccacttt tgtgctctga cttccgccat
     1561 caatccctat gatgtatttc ttcgacatga tttttctcct gtaattttcc tggcttactt
     1621 gctgctggcc attgcggggg cgctatcagc agtacgaatt ttttggaaca gcaaaaaggt
     1681 aaagacaatc accatggcaa gcgccgccat gcccatcagc cacatattgc ggtaggcttc
     1741 tgctgcgggc tgggtgtctt gccagtggcc gataattggg taaacgaaga catccggcag
     1801 gaatccaatc accgagcaaa tacctaccgt ggtgcccata atgtaagacg gtgttctcgc
     1861 ttcgccaggg caggcccagt acagcccgcg tgaggcgtaa caggtgaatc ccagcagtaa
     1921 aatcaggcca atccccatcg cgaccgattg cgggttagag ttcgtgacga gcagggcagt
     1981 taacgccagc aggccgagta cggaaaggat ttggatcacg cgggtagggg atttcacttt
     2041 actgtaggtg gtgataatgc cgccaagtgg gccgcacagc gcgcggaaga ttttgttgat
     2101 cacaatcccc atgtagctcg ccgccaccag cgacatgcca tacatttcgg tcagatagtt
     2161 ggtggagtaa ctcagaatgg cgtagatggt gaagacgcca aaaatcacca tgctgcaata
     2221 ccaggtggtg ctgatacgca aaacggcaag gatgtcgcta agctggaatg actgtttttc
     2281 ttcgttattg gcactgcgca ggttgttgtt atcgctaaca aaaaaccagc acagaatccc
     2341 caacaagatg taaaccacac tatagatgat aatgaccgtt ttcaggctgg tgctgtcatc
     2401 cggtgcaaag cgagaaaaaa cccacatggt aaacactgcc agcgacatta caccgacgcc
     2461 gcgcagccct tccatccagc ccataatttt cccttgctcg ctatgatcgc caagcaacga
     2521 tgcggcttta atcgacaccg accacagcat taaaatcgtt gttatcgcaa aggcgacctg
     2581 aatacagagc attacccaca gcggtggata cgttgccatt aacagaccca gtaatccggt
     2641 aatgatcatc gcggaagtaa tcattttgcg gtgtgaaaat ttatcggcaa taacgccgct
     2701 gggggcataa agaataatag ccgcaatacc aaaggtactc attattaaac ctatctcggt
     2761 attgctgaat cccataaatt tcgccatggg aatttgataa atataacgta aataagccag
     2821 gtcgaaacta acgccgccgc taaaactaat tatcgcgagg gttatccaac ggcgatatga
     2881 gttgtgttgc atatcgtatt cccatttgta atagacaaaa aaaagagaaa agtaagccgc
     2941 cctttcaggt ggattacttt tctcaggtct ctag
//
LOCUS       DBFIFI010000328         2949 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.330, whole genome shotgun sequence.
ACCESSION   DBFIFI010000328 DBFIFI010000000
VERSION     DBFIFI010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.330"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            1..368
                     /locus_tag="N7B39_004740"
                     /pseudo
     CDS             1..368
                     /locus_tag="N7B39_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            384..2864
                     /gene="yehB"
                     /locus_tag="N7B39_004741"
     CDS             384..2864
                     /gene="yehB"
                     /locus_tag="N7B39_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HGU2424561.1"
                     /translation="MLRMTPLASAIVALLLGIEAHAAEETFDTHFMMGGMKGEQVTNL
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPHETCLTREIVKRLGINSDNFAREN
                     QCLTFEQLVQGGSYSWDIGIFRLDLAVPQAWVEELENGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTDNNPGEWKSNTLYLEHGF
                     SQILGTLRIGDMYTSADIFDSVRFTGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFSISDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFLSQTSTRFGLAAWRYSSRDYRT
                     FNDYVWANNKDNYRRDKNDVYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYTLAASQAYDENHAEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGNRDQFNYGINLSHQHQGNETTAGANL
                     TWTAPAATVNGSYSQSSTYRQVGASVSGGLVAWSGGVNLANRLSETFAVMHAPGIKDA
                     YVNGQKYRTTNCNGVVVYDGLTPYRENHLMMDVSQSDSETELRGNRKMTAPYRGAVVL
                     VDFDTDQRKPWFIKALRSDGQPLTFGYEVNDMHGHNIGVVGQGSQIFIRTNEIPPAVN
                     VAIDKQQGLSCTITFGKEIDESKNYICR"
     gene            2875..>2949
                     /locus_tag="N7B39_004742"
     CDS             2875..>2949
                     /locus_tag="N7B39_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424562.1"
                     /translation="MMFRNRILLIFILWANFTWAGCRTT"
BASE COUNT          855 a          547 c          731 g          816 t
ORIGIN      
        1 ttttttatct taatgttctg gatattccac cgaatagccc agaagatgaa ggaaagaatg
       61 cgttaaaatt tgccatgtaa aacagaatca agttgtttta ccgacctgct ggtattgctc
      121 cggtaaataa aacgactttt aaaaaattac gagtgagtcg tagcggtaat aacgtagtca
      181 taaaaaatga ctcgccaaat tgggtaacga tttctgatgt taaggctaat aacgtcaaag
      241 tgaattatga aacaatcatg attgcaccac aagaaagtca gaatgttagt gaaaaaaata
      301 ataatgcgaa tggctggcaa ctgaccatta tcgatgatca cggcaactat attagtgaca
      361 aaatttaatt gcaggagctg cctatgttga gaatgacccc gcttgcatca gcaatagtcg
      421 cgttattgct cggcattgaa gcacatgcag cagaagaaac ctttgacacc cattttatga
      481 tgggaggaat gaaaggtgag caagtaacaa atttgcgtct tgatgataat cagcctttac
      541 ctgggcagta cgacattgat atttatgtca ataaacaatg gcgtggaaaa tatgagatta
      601 tcgtcaaaga taacccacat gaaacttgcc tgacgcgtga aattgtaaag cggttaggta
      661 taaatagcga taattttgca cgcgaaaatc agtgtttaac atttgagcaa cttgttcagg
      721 gcggaagcta ctcctgggat attgggattt ttcgtttgga tctcgctgtt ccgcaagcct
      781 gggttgaaga gctggaaaat ggttacgttc cgccagaaaa ctgggagcgg ggaatcaatg
      841 cgttttatac gtcttattac gtaagtcagt attacagtga ctataaagcc tcgggcaata
      901 gtaagagcac ttatgtgcgt tttaacagtg ggctaaattt gctgggatgg cagctgcatt
      961 cagatgccag ttttagcaaa acggataata atcctggtga gtggaaaagc aacacgctgt
     1021 atctggagca tggattttct caaattctcg gtacgctgcg tattggtgat atgtacacct
     1081 cagccgatat atttgattcc gttcgcttca ccggggttcg gttatttcgt gatatgcaga
     1141 tgttgccaaa ctcgaagcaa aattttaccc cacgggtaca ggggattgcc cagagtaacg
     1201 cgttggtaac tattgaacag aacggttttg tcgtttatca gaaagaggtt ccaccaggtc
     1261 cgttttcgat tagtgatttg cagttagcgg gcggaggggc ggatcttgat gttagtgtta
     1321 aggaagccga tggttctgtg actacgtatc tggtgcctta tgcggcagta cctaatatgc
     1381 tgcaacctgg tgtatcaaaa tatgattttg cggcaggccg tagtcatatt gaaggtgcga
     1441 gcaagcaaag tgattttgtc caggcgggtt atcagtatgg ttttaataat ttattgacgc
     1501 tgtatggcgg cacgatggtt gctaataatt actatgcctt taccctcgga acaggttgga
     1561 acacgcgtat tggcgcaatt tcagtcgatg caactaaatc gcacagtaag caagacaatg
     1621 gtgatgtgtt tgacggacaa agttatcaaa ttgcctataa caaatttttg agccaaacat
     1681 caacacgttt tggtctggcg gcctggcgtt attcgtcgcg tgattaccgc acatttaacg
     1741 attatgtgtg ggcaaataat aaagataatt atcgtcgtga taaaaacgat gtctatgata
     1801 ttgccgatta ttaccagaat gattttggtc gcaaaaatag cttttccgcc aatatgagtc
     1861 agtcattgcc agaaggctgg ggatctgtgt cattaagtac tttatggcgt gattactggg
     1921 ggcgaagcgg cagtagtaaa gattatcaac tgagttattc caataactgg cggcggataa
     1981 gctataccct cgcggcaagc caggcgtatg acgagaacca cgccgaagag aaacgcttta
     2041 atatttttat atcgattccc tttgactggg gcgatgacgt tacgacgccg cgacggcaaa
     2101 tatatatgtc taactcaacg acctttgatg atcagggttt tgcttcaaac aataccggat
     2161 tatcgggaac cgttgggaac cgcgatcagt ttaactatgg tatcaacttg agccatcaac
     2221 atcagggaaa tgaaacgaca gctggggcca atttgacctg gaccgcaccg gccgcaacag
     2281 tgaatggcag ctatagtcag tcgagtactt atcgacaggt cggagccagt gtttcagggg
     2341 gactggttgc ctggtctggt ggcgttaatc tggcgaaccg tctttccgaa acgtttgctg
     2401 tgatgcatgc gccgggaatc aaagatgctt atgtcaatgg gcaaaaatat cgtacaacaa
     2461 actgtaatgg tgtggtggtg tacgacggac tgacacctta tcgggaaaat cacctgatga
     2521 tggatgtgtc gcaaagcgat agcgaaacag aattacgcgg gaaccgtaaa atgaccgccc
     2581 cttatcgcgg cgcggtcgtt ctggttgatt ttgataccga tcagcgtaag ccctggttta
     2641 taaaagcatt aagatccgat ggacagccat taacgtttgg ttatgaagtc aatgatatgc
     2701 atggtcataa cattggtgtt gtcggccagg gcagtcagat atttattcgc accaatgaaa
     2761 taccgccagc ggttaatgtg gcaattgata agcaacaagg gctttcatgc acaatcacct
     2821 tcggtaaaga gattgatgaa agtaaaaatt atatctgtcg gtgagaggtg aaatatgatg
     2881 ttcagaaata gaatattact aatatttata ttgtgggcta attttacctg ggctgggtgt
     2941 cgtactact
//
LOCUS       DBFIFI010000329         2948 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.331, whole genome shotgun sequence.
ACCESSION   DBFIFI010000329 DBFIFI010000000
VERSION     DBFIFI010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.331"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(66..482)
                     /locus_tag="N7B39_004743"
                     /pseudo
     CDS             complement(66..482)
                     /locus_tag="N7B39_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(482..667)
                     /locus_tag="N7B39_004744"
     CDS             complement(482..667)
                     /locus_tag="N7B39_004744"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424563.1"
                     /translation="MDVRNKKLVFWFVRVDDEGYPEIARCTEREFATILAGISAGGMY
                     CPECGTVHWPDGVPPPF"
     gene            844..>2948
                     /gene="katP"
                     /locus_tag="N7B39_004745"
     CDS             844..>2948
                     /gene="katP"
                     /locus_tag="N7B39_004745"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase KatP"
                     /protein_id="HGU2424564.1"
                     /translation="MIKKTLPVLILLALSGSFSTAVAADKKETQNFYYPETLDLTPLR
                     LHSPESNPWGADFDYATRFQQLDMEALKKDIKDLLTTSQDWWPADYGHYGPFFIRMAW
                     HGAGTYRTYDGRGGASGGQQRFEPLNSWPDNVNLDKARRLLWPVKKKYGSSISWGDLM
                     VLTGNVALESMGFKTLGFAGGREDDWESDLVYWGPDNKPLADNRDKNGKLQKPLAATQ
                     MGLIYVNPEGPGGKPDPLASAKDIREAFSRMAMDDEETVALIAGGHTFGKAHGAASPE
                     KCIGAGPDGAPVEEQGLGWKNKCGTGNGKYTITSGLEGAWSTSPTQFTMQYLKNLYKY
                     EWELHKSPAGAYQWKPKKAANIVQDAHDPSVLHPLMMFTTDIALKVDPEYKKITTRFL
                     NDPKAFEQAFARAWFKLTHRDMGPAARYLGNEVPAESFIWQDPLPAADYTMIDGKDIK
                     SLKEQVMDLGIPASELIKTAWASASTFRVTDYRGGNNGARIRLQPEINWEVNEPEKLK
                     KVLASLTSLQREFNKKQSDGKKVSLADLIVLSGNAAIEDAARKAGVELEIPFTPGRTD
                     ASQEQTDVASFSVLEPTADGFRNYYSKSRSHISPVESLIDKASQLDLTVPEMTALLGG
                     LRVMDINTNNSSLGVFTDTPGVLDNKFFVNLLDMSTRWSKADKEDTYNGFDRKTGALK
                     WKASSVDLIFSSNPELRAVA"
BASE COUNT          795 a          665 c          759 g          729 t
ORIGIN      
        1 aggagcgaac gtctggttca gcagatttgg cgcaactggc agattgtggt tcgagttcgt
       61 agtcgcacat ctgcctggcg gcagatttcc tgtatcacat ccgcagcaat gcggctcatc
      121 tctgccagta accaccggtt gtggaacacc agggaccagt actggcaggg aagtgtgaac
      181 acaatatgct gccacggaca gtcgggaacc agactcagca gatactgtat ccactgtgcg
      241 ccagccttca ctccgcagtg cgggcaggag cgacttttac accggaagca gacctttttt
      301 atgtggctgc agtccggaga tgaacagcac cactgtgtgt accccatcag cgtggtcccg
      361 caggccatga tttttgtcac agactcaatg actaccggac gcactgaacc ttccggttgt
      421 ttctccagcc agttaagcca gcggtttccc tgctgaaaaa tgtcggcaaa acggggaagc
      481 atcagaaggg cgggggaact ccgtccggcc agtgaaccgt gccacactcc gggcagtaca
      541 tgccgccggc gctgataccg gcaagaatgg tcgcaaactc ccgctccgtg cagcgggcta
      601 tttcaggata cccttcgtca tcaacacgta caaaccagaa gaccagcttt ttgtttctga
      661 catccacaaa gaagggaata ttcaggtctg cgcagcactc aacggcatcg tcagttgcgg
      721 cttggaaccc cttagtattt tttgtctgta gtatctatcc cagcaatagg tatatcctgt
      781 tgcatcaata aagttgactt ttgtatacaa catgcgaatt tcccttaatc cggagctatt
      841 cgtatgataa aaaaaactct tcctgttctg attcttctgg cgctatcggg gagcttttct
      901 accgctgtag ccgctgataa aaaagagact caaaatttct actatccaga aacactggat
      961 ttaactcctc tgagattaca cagccctgaa tcaaatccct ggggggctga ttttgattat
     1021 gccaccagat ttcaacagct ggatatggag gctctgaaaa aagatatcaa agatttgctg
     1081 acaacttccc aggattggtg gcctgcggat tatggtcatt atggtccttt ctttattcgt
     1141 atggcttggc acggtgccgg aacatacagg acatatgatg gccggggagg cgccagtggt
     1201 ggtcagcaac gttttgaacc gctgaacagc tggccggata acgttaatct ggataaagcc
     1261 cgtcgattgc tgtggccagt caagaaaaaa tacggctcca gtatttcctg gggagacctg
     1321 atggtcctga ctggtaatgt tgcccttgaa tccatgggat ttaaaacgct gggatttgct
     1381 ggcggaagag aagatgactg ggagtcggac ctggtatact gggggcctga caacaagcct
     1441 cttgcagata accgggataa aaacgggaaa cttcagaaac ctcttgccgc cacgcagatg
     1501 ggacttattt atgtcaatcc tgaaggcccc ggtggaaaac cagatcctct ggcttccgcg
     1561 aaagatatca gggaagcttt ttcacgtatg gccatggatg atgaggagac tgtggccctg
     1621 atcgcgggag ggcatacatt tggtaaagca catggtgcag cgtctcctga aaaatgtatt
     1681 ggcgcagggc ctgatggtgc acctgtggag gagcagggac tgggatggaa aaataaatgt
     1741 ggtacaggaa acggcaaata taccatcacc agtggcctgg aaggagcctg gtcgacatcg
     1801 ccaacccagt tcacaatgca gtatctgaag aatttatata aatatgaatg ggagctgcac
     1861 aagagtcctg ccggtgctta tcagtggaag cctaaaaaag cggcaaatat agttcaggac
     1921 gcgcatgatc cgtctgtcct gcatccgttg atgatgttta cgacggatat tgctcttaaa
     1981 gttgatcctg aatataagaa aataaccacc cgtttcctga atgatccaaa agcttttgag
     2041 caggcattcg caagagcatg gtttaaactg acccaccggg atatgggacc ggcagcccga
     2101 tatcttggta atgaagttcc tgcagaatca tttatctggc aggatcctct tcctgcggcg
     2161 gattatacaa tgattgatgg taaagacatt aagtcgctga aagagcaggt tatggatttg
     2221 ggtatccctg catctgagct gataaagaca gcctgggctt cagcttccac atttcgtgtg
     2281 actgattatc gtgggggaaa taatggtgcc cgcatcaggt tacagcccga aattaactgg
     2341 gaagttaatg agcctgaaaa actgaagaaa gtactggcat ccctgacctc attacagcgt
     2401 gaatttaaca aaaaacagtc tgacggaaag aaagtgtcgt tggctgattt aattgttctt
     2461 tcgggtaatg ctgcaatcga agatgcggcc agaaaagccg gggtggaact tgagattccc
     2521 tttactccgg gaagaactga cgcctctcag gagcagacgg atgttgcctc attcagtgta
     2581 ctggagccga cagcagatgg attcagaaat tattactcaa aaagcagaag tcatatatcg
     2641 ccggttgaaa gcctcattga taaagccagt cagctggatc tcaccgttcc tgaaatgacg
     2701 gcattactgg gtggtctgcg ggtaatggat attaatacaa ataattcttc gttgggagtg
     2761 tttaccgata cccctggtgt tctggataac aagttttttg ttaatctgct ggatatgtca
     2821 acacgatgga gtaaagcaga taaagaagat acatacaatg gattcgatcg taaaacggga
     2881 gcattaaaat ggaaagcatc ctctgttgat ttaatcttca gttcaaatcc tgaattacgt
     2941 gcggtggc
//
LOCUS       DBFIFI010000330         2945 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.332, whole genome shotgun sequence.
ACCESSION   DBFIFI010000330 DBFIFI010000000
VERSION     DBFIFI010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.332"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            79..2169
                     /gene="iha"
                     /locus_tag="N7B39_004746"
     CDS             79..2169
                     /gene="iha"
                     /locus_tag="N7B39_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HGU2424565.1"
                     /translation="MRITTLASVVIPCLGFSASSIAAAEDVMIVSASGYEKKLTNAAA
                     SVSVISQEELQSSQYHDLAEALRSVEGVDVESGTGKTGGLEISIRGMPASYTLILIDG
                     VRQGGSSDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITRKN
                     ADKWLSSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSVSLQVRGSTQQRQGSSVTSL
                     SDTAATRIPYPTESQNYNLGARLDWKASEQDVLWFDMDTTRQRYDNRDGQLGSLTGGY
                     DRTLRYERNKISAGYDHTFTFGTWKSYLNWNETENKGRELVRSVLKRDKWGLAGQPRE
                     LKESNLILNSLLLTPLGESHLVTVGGEFQSSSMKDGVVLASTGETFRQKSWSVFAEDE
                     WHLTDALALTAGSRYEHHEQFGGHFSPRAYLVWDVADAWTLKGGVTTGYKAPRMGQLH
                     KGISGVSGQGKTNLLGNPDLKPEESVSYEAGVYYDNPAGLNANVTGFMTDFSNKIVSY
                     SINDNTNSYVNSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTRSEQRDGDNKGAPLSY
                     TPEHMVNAKLNWQITEEVASWLGARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAW
                     TVVDAGLSWKMTDALTLNAAVNNLLNKDYSDVSLYSAGKSTLYAGDYFQTGSSTTGYV
                     IPERNYWMSLNYQF"
     gene            complement(2387..2537)
                     /locus_tag="N7B39_004747"
                     /pseudo
     CDS             complement(2387..2537)
                     /locus_tag="N7B39_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2749..2945)
                     /locus_tag="N7B39_004748"
                     /pseudo
     CDS             complement(2749..2945)
                     /locus_tag="N7B39_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053346.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
BASE COUNT          762 a          682 c          793 g          708 t
ORIGIN      
        1 aggcaaatat aaattattct cattattgtt tgtatttgtg tattgtcttg ccggttaaca
       61 tgatcggaga ttagtaatat gcgaataacc actctggctt ccgtagtcat tccctgtctc
      121 ggattttcag ccagcagcat agctgctgca gaggatgtga tgattgtctc ggcatccggc
      181 tatgagaaaa agctgactaa cgcagccgcc agtgtttctg tgattagcca ggaggaattg
      241 cagtccagcc agtaccacga tctggcggag gctctgagat cagtagaggg tgtggatgtt
      301 gaaagtggta cgggtaaaac cggagggctg gaaatcagca tccgaggaat gccagccagt
      361 tacacgctga tactgattga tggtgttcgt cagggcggaa gcagtgacgt gactcccaac
      421 ggtttttctg ccatgaatac cgggttcatg ccccctctgg ccgccattga gcgtattgag
      481 gttatcaggg ggccgatgtc cacactgtat ggctctgatg cgatgggcgg tgtggtgaat
      541 atcattacca gaaagaatgc agacaaatgg ctctcttccg tcaatgcagg gctgaatctg
      601 caggaaagca acaaatgggg taacagcagc cagtttaatt tctggagcag tggtcccctt
      661 gtggatgatt ctgtcagcct gcaggtacgc ggtagcacac aacagcgtca gggttcatcg
      721 gtcacatcac tgagcgatac agcagccacg cgtattcctt atcccacgga gtcacagaat
      781 tataatcttg gtgcacgtct tgactggaag gcgtcggagc aggatgtgct ctggtttgat
      841 atggatacca cccggcagcg ttatgataac cgggatgggc aactggggag tctgacgggg
      901 ggatatgacc ggaccctgcg ctatgagcga aacaaaattt cagctggcta tgatcatact
      961 ttcaccttcg gaacatggaa atcgtatctg aactggaacg agacagaaaa taaaggtcgt
     1021 gagcttgtac gcagtgtact gaagcgcgac aaatgggggc ttgccggtca gccgcgggag
     1081 cttaaggaat cgaaccttat cctgaattca ttactgctta cccctctggg agaatctcat
     1141 ctggttacgg tggggggcga gtttcagagc tcgtccatga aagacggagt tgtccttgcc
     1201 agcacaggtg aaactttccg gcagaaaagc tggtcggtat ttgctgagga tgagtggcat
     1261 ctcacggatg cacttgcgct gactgcgggc agccgctatg aacatcatga gcaattcggg
     1321 ggacacttca gtccgcgtgc atatctggtc tgggatgtgg cagatgcctg gacgctgaaa
     1381 ggcggtgtga ccacgggata taaggcaccc agaatggggc agctacataa agggattagt
     1441 ggtgtgtccg ggcagggaaa aacaaatcta cttggtaacc ccgacctgaa gccggaagag
     1501 agcgtcagtt atgaggctgg ggtgtattac gataaccccg ccggtctgaa tgccaatgtc
     1561 acaggtttta tgactgactt ctccaacaag attgtctctt attccataaa tgataacacc
     1621 aatagctatg taaacagcgg aaaggcccgg ttgcacggtg tggaatttgc cggcacattg
     1681 ccgctgtggt cagaggatgt cacgctgtca ctgaattaca cctggacccg aagtgaacaa
     1741 cgtgatggtg ataacaaagg tgcgccgctg agttataccc ctgaacacat ggtgaatgcg
     1801 aaactgaact ggcagatcac cgaagaggtg gcatcatggc tgggtgcccg ttatcgcggg
     1861 aaaacaccac gtttcaccca gaattattcg tcactgagcg ctgtacagaa gaaagtgtat
     1921 gatgagaaag gagaatacct gaaagcctgg acggtggtgg atgcaggtct gtcgtggaag
     1981 atgacggatg ccctgacgct gaatgctgcg gtgaataacc tgctcaacaa ggattacagt
     2041 gacgtgagcc tgtacagtgc cggtaagagt acgctgtatg ccggtgatta cttccagacg
     2101 ggatcatcaa caacaggata tgtgatacct gagcgaaatt actggatgtc gctgaactat
     2161 cagttctgat aataacaaaa cgctatcact gacggtagaa tacgttgcca ctgcaactcc
     2221 tggcggaaca gtggcaacgt attaggttaa gtgcatttcc gatccgctaa tgagatttcg
     2281 ttaccaacaa ctaatatcgt cacaggaaat gcacggatta tttttaactt atcatttaca
     2341 tacttgtcca gagtgtaagc gcaccgtgaa ggacgtgggg taaaaattag tttacagaga
     2401 gagtgacgtt ccagggcaac aactctttca tgcggttggc aggccaggtg ttgattacac
     2461 tgatcacgtg gcgtagccac gtttccggct cgattccgtt aagttttgca gctaccgatc
     2521 aggctgtaca tcactgccgc actatcgctc gtcatctcaa agtccggtct cgtcagcagg
     2581 aaggtatcat tctctcccgc catttttcca ggggcccggt cagataagtc cctttgtcta
     2641 tcgctgactc ctgactcata acccggttag cagaatgcag ggtcaccacc tcgccacgac
     2701 caaatccaaa taagtcaatt gcaccttctc aatcgccatt ttgtcagtaa gcgtacagcc
     2761 tgaactgatg gtatcttcac catcaatgac aacggtgatc gcaattttac tgacgttcgc
     2821 cggaacacga tccagtgcta tctcaatgct ggcctgctgc gaaccggtaa cgagcctgac
     2881 tgccccctca ggagaagaca aattattata aaagataaag tcagaatcgc cactgacctt
     2941 tccct
//
LOCUS       DBFIFI010000331         2941 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.333, whole genome shotgun sequence.
ACCESSION   DBFIFI010000331 DBFIFI010000000
VERSION     DBFIFI010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.333"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(191..267)
                     /locus_tag="N7B39_004749"
     tRNA            complement(191..267)
                     /locus_tag="N7B39_004749"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(231..233),aa:Asp,seq:gtc)
     gene            complement(400..1131)
                     /gene="dnaQ"
                     /locus_tag="N7B39_004750"
     CDS             complement(400..1131)
                     /gene="dnaQ"
                     /locus_tag="N7B39_004750"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HGU2424566.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRLVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            1196..1663
                     /gene="rnhA"
                     /locus_tag="N7B39_004751"
     CDS             1196..1663
                     /gene="rnhA"
                     /locus_tag="N7B39_004751"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HGU2424567.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1660..2382)
                     /locus_tag="N7B39_004752"
     CDS             complement(1660..2382)
                     /locus_tag="N7B39_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGU2424568.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFY
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2416..>2941
                     /gene="gloB"
                     /locus_tag="N7B39_004753"
     CDS             2416..>2941
                     /gene="gloB"
                     /locus_tag="N7B39_004753"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HGU2424569.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFA"
BASE COUNT          789 a          708 c          702 g          742 t
ORIGIN      
        1 tctggaaata tatttaagct gaatggttcg atatcattat tgaattttaa tgaatgagac
       61 aacaagcgag aatcacgcac ttacgccaga gtaaaatcat tgaacagaga aatggtggaa
      121 ttcaaataca aaaaaaaccg caaaattaaa aatcttgcgg ttctctgaac tcattttcat
      181 gaggtgaatt tggcggaacg gacgggactc gaacccgcga ccccctgcgt gacaggcagg
      241 tattctaacc gactgaacta ccgctccgcg ttgtgttccg ttgggaacga ggcgaatatt
      301 acggattgcc tcacccttcg tcaacggttt ttctcatctt ttgaatcatt tgctgcaaaa
      361 atcgcccaag tcgctatttt tagcgccttt tacatgtatt tatgcccgcc agaggcagct
      421 tccgcccttt ttctgcacca gatcgagacg ggcttcatgt gctgcaagct cttcatctgt
      481 cgcaaaaaca acgcgtaact tacttgcctg acgtacaagg cgctggattg ttgcttcacc
      541 ttgttgctgt tgtgtctctc cttccatcgc aaaagccatc gacgtttgac caccggtcat
      601 cgccagataa acttccgcaa ggatctgggc atcgagtaat gccccgtgca gcgttcgttt
      661 actgttatct atttcgtagc gggcacataa cgcatcgagg ctgttgcgtt taccgggaaa
      721 cattttcctc gccaccgcaa ggctatcggt gaccttacag aaggtattag tcttcggaat
      781 atcgcgctta agcaacgaga actcgtagtc cataaagccg atatcgaacg ctgcgttatg
      841 gatcaccaac tccgcgccgc ggatatagtc catgaactca tcggctactt cggcaaacgt
      901 gggcttatcg agcaaaaatt catcggcaat accatgtacg ccaaaggctt ccggatccac
      961 cagccgatcg ggtttgagat aaacatggaa gttattgccc gtcaggcgac ggttcaccac
     1021 ttcaacggca ccaatctcaa tgatcttgtg gccttcatag tgcgcaccaa tctggttcat
     1081 accggtggtt tcggtatcga gaacgatctg gcgtgtaatt gcagtgctca tagcggtcat
     1141 ttatgtcaga cttgtcgttt tacagttcga ttcaattaca ggaagtctac cagagatgct
     1201 taaacaggta gaaattttca ccgatggttc gtgtctgggt aatcctggac ctgggggtta
     1261 cggcgctatt ttacgctatc gcggacgcga gaaaaccttt agcgcaggct acacccgcac
     1321 caccaacaac cgtatggagt tgatggccgc tattgtcgcg ctggaggcgt taaaagaaca
     1381 ttgcgaagtc attttgagta ccgacagcca gtatgtccgc cagggtatca cccagtggat
     1441 ccataactgg aaaaaacgtg gctggaaaac cgcagacaaa aaaccagtaa aaaatgtcga
     1501 tctctggcaa cgtcttgatg ctgcattggg gcagcatcaa atcaaatggg aatgggttaa
     1561 aggccatgcc ggacacccgg aaaacgaacg ctgtgatgaa ctggctcgtg ccgcggcgat
     1621 gaatcccaca ctggaagata cgggctacca agtcgaagtt taagcctgtg gtttacgaca
     1681 ttgccgggtg gctccaaccg cctggcgaat tcgtggcttg tttttactct gtttcatcgg
     1741 atttagcgtt aaaggaatag tccgtttccg ggcaacaata agttgtaagc aaccaagcgc
     1801 aggaatatgc gcattcaaaa gttttcctcc gtgtttgttc cacgggagaa cgtggaaacg
     1861 gctggcgtgt agcacttcaa aattcaacaa agagagccag tccagttgcc gcatcagagt
     1921 aaacatccgg ctgttatagg gcgaggtttt gcgcaatacc ggcacaagtt tgcgtaatcc
     1981 cattaaacta atgggattga agccactaat gaccagccag ccatcatcaa tcaatacccg
     2041 atcggcttca cgcaataaac gatgcggatc ggtgcaccac ggcaatgtat gtgccagtag
     2101 acaaacatca acggatttat cggcaaaagg aagatgaagt gggtccgcct gcacctggac
     2161 aggcattcct tgcgcagaaa cattcacttg atgagaaacc gcgcacgctt cgcaattgat
     2221 ttctgcgctt aaattgccaa tcttaagcag ataaaaacca tacattttag tgaaccacgg
     2281 gttgagctgg cgctccagcg ccttgcgata aagctctccc cagggcaaat cgccccagcg
     2341 atcaggagcc acgacagttt gagggactct tgccggtttc atcacaacct tccgtttcac
     2401 actgagaggt aatctatgaa tcttaacagt attcccgcct ttgatgacaa ttacatctgg
     2461 gttttgaatg atgaagcagg tcgctgcctg attgtcgatc ccggagacgc agagccagta
     2521 ttaaacgcca ttgccgccaa taactggcaa ccggaggcca tatttctcac ccaccatcat
     2581 cacgatcacg ttggcggcgt aaaagaactg gtggaaaagt ttccgcaaat tgtggtgtat
     2641 ggtccacaag agacacaaga taagggaaca acacaggtag tcaaagatgg cgaaactgcc
     2701 ttcgttttgg ggcatgaatt tagtgtaatt gccacgccgg gtcacacttt aggacatatc
     2761 tgttacttca gtaaacctta tctattttgc ggcgacacgc tgttttctgg tgggtgtggt
     2821 cggttgtttg aagggacagc atcacaaatg tatcaatcac ttaaaaagtt aagtgcgtta
     2881 cctgacgata cattggtatg ttgtgctcat gaatatacgt tatcaaatat gaagtttgct
     2941 t
//
LOCUS       DBFIFI010000332         2906 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.334, whole genome shotgun sequence.
ACCESSION   DBFIFI010000332 DBFIFI010000000
VERSION     DBFIFI010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.334"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..2399
                     /locus_tag="N7B39_004754"
     CDS             <1..2399
                     /locus_tag="N7B39_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HGU2424570.1"
                     /translation="TAIEAGDERRARAKFHWQFLEHYPAAQDCAYKFLVCEDKPGIPR
                     PALDSWDAEYMQENRWDEGAASFVPVETESDPMNVAFDKLAPEVQNAVMVKFDTCENI
                     TVDMVISAQELLQEDMATFDGHIVEALMKMPEVNAMYPELKLHAIGWVKHKCKPGAKW
                     PEIQAEMRIWKKRREGERKETGKYTSVVDLARARVHRQHTENSAEKIPPVTAVIRREY
                     KQTWKTLDDELAYALWPGDVDAGNIDGSIHRWAKNEVIDNDREDWKRISASMRKQPDA
                     LRYDRQTIFGLVRERPIDIHKDPVALNKYITEYLTTKGVFEDEGTNQSATDTLSSPVP
                     ETDAVETAIPDNEKTECKVEVEPSVEREGPFYFLFTDKDGEKYGRANKLSGLDKALAA
                     GATEITKEEYFARKNGTYTGLPQNANTAQNSEQPEPVKVTADEVKKIMQAANISQPDA
                     NQLLAASRGEFVAGISDPNDPKWVKGIETRDSVNQNQQETEQNDQKAEQNSPNTQQNE
                     PETKQPEPVAQQEPEKVCTACGQTGGGNCPDCGAVMGDATYQETFDEEYQVEVQEDDP
                     EEMEGAEHPHKENTDGNQHHDSDNETGETADHSIKVNGHQEITSTSRTCDHLMIDLET
                     MGKNPDAPIISIGAIFFDPQTGDMGPEFSKTIDLETAGGVIDRDTIKWWLKQSREAQS
                     AIMTDEIPLDDALLQLREFIDENSGEFFVQVWGNGANFDNTILRRSYERQGIPCPWRY
                     YNDRDVRTIVELGKAIDFDARTAIPFEGERHNALDDARYQAKYVSVIWQKLIPNQADF
                     "
     gene            2464..2712
                     /locus_tag="N7B39_004755"
     CDS             2464..2712
                     /locus_tag="N7B39_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HGU2424571.1"
                     /translation="MARLILLTEWAKEEFSEPVPTPSTLSKYAKAGMIFPLPKKVGRR
                     WRVDPQARFVGMVNKPEVIATDHPALKRILEDGAPAKI"
     gene            2690..>2906
                     /locus_tag="N7B39_004756"
     CDS             2690..>2906
                     /locus_tag="N7B39_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HGU2424572.1"
                     /translation="MARPRKYKTDVPGLSPYFDKRNNKVYWRYRHPITGKNHGLGSID
                     QKLAETIAAEANSRLARQQMEQMLSLQE"
BASE COUNT          882 a          708 c          733 g          583 t
ORIGIN      
        1 ccactgccat tgaagccggt gatgaacgtc gcgcccgcgc aaaatttcac tggcaattcc
       61 tggagcatta tccggctgct caggactgcg cttataaatt tcttgtttgc gaggataaac
      121 ccggtatacc ccgccctgcc ctcgattcct gggatgctga atatatgcag gaaaaccgct
      181 gggatgaggg ggctgcttcc tttgtcccgg ttgagactga atccgatccg atgaacgtcg
      241 cttttgacaa gctggcccct gaagtacaga acgctgtcat ggttaagttc gacacatgtg
      301 aaaacatcac cgttgatatg gtgattagcg cgcaggaatt gttgcaggaa gacatggcaa
      361 cattcgacgg acatatcgtt gaagcgttga tgaaaatgcc agaagttaac gccatgtatc
      421 cggagcttaa gctgcatgcc atcgggtggg ttaagcataa atgtaagcct ggtgccaaat
      481 ggcccgaaat tcaggcagaa atgcgcatct ggaaaaaacg tcgcgaaggt gaacgcaagg
      541 aaaccggaaa atacacgtct gttgttgatc tcgcccgcgc cagagtccac cgacagcaca
      601 ctgaaaactc agcagaaaaa atcccccctg tcactgcagt cattcgtcgc gaatataagc
      661 agacatggaa aacactggat gacgaactgg cctacgctct ctggcctggt gatgtggatg
      721 ccggaaacat tgacggcagc atccatcgct gggcaaaaaa tgaagttatc gacaacgacc
      781 gcgaagactg gaagcgtatc tcggcatcaa tgcgcaaaca gcctgatgcc cttcgctacg
      841 accgccagac tatttttggc cttgtccgtg aacgtccgat cgacattcac aaagaccctg
      901 tggcactgaa caaatacatt actgaatacc tgactacaaa gggcgtgttt gaagatgaag
      961 gaacaaatca gagcgcaact gacactctct cgtcgccagt accagaaact gatgcagtgg
     1021 aaacggcaat tccggacaac gaaaaaaccg aatgcaaagt ggaagtcgaa ccatctgtag
     1081 agcgtgaggg gccgttctac ttcctcttca ccgacaagga tggcgaaaaa tacggtcgcg
     1141 caaacaaact ttctggtctg gataaggcgc tggctgccgg ggctactgaa atcacaaaag
     1201 aagaatattt tgcccgaaaa aatggcacat acacaggctt accgcaaaat gcaaataccg
     1261 cacaaaattc tgaacaacca gaaccggtaa aagttaccgc tgacgaagta aagaaaatta
     1321 tgcaggcagc caatatcagc cagcctgacg ccaatcagtt gctcgccgca tcacgtggtg
     1381 aatttgttgc agggattagc gacccgaatg atccgaaatg ggtgaagggg attgaaaccc
     1441 gcgattctgt gaaccagaac cagcaagaaa cggaacagaa cgaccagaaa gcggaacaaa
     1501 acagcccaaa tacgcaacaa aacgagccag aaacgaaaca acctgagcca gtagcgcaac
     1561 aggaaccgga aaaagtctgc accgcctgcg gtcagaccgg cggcggcaac tgccctgatt
     1621 gtggcgcggt gatgggcgac gcaacatacc aggaaacatt cgatgaagag tatcaggttg
     1681 aagttcagga agatgatccg gaggaaatgg aaggcgctga acatccacac aaggagaaca
     1741 ctgacggcaa tcagcatcac gatagcgata atgaaactgg cgagacggca gatcactcaa
     1801 ttaaggtgaa cggtcatcaa gaaatcacat ccaccagcag gacgtgtgac catctaatga
     1861 tcgaccttga aaccatggga aaaaatcctg atgccccgat catctcaata ggtgcaatat
     1921 ttttcgatcc gcaaaccgga gatatgggac cggaatttag taagactatc gatctggaaa
     1981 ctgctggcgg ggtcattgat cgggacacca ttaaatggtg gcttaagcaa tcacgcgaag
     2041 cgcaatctgc cattatgacc gatgaaatcc cgttagatga tgcactatta caattgcggg
     2101 aatttatcga cgaaaactcc ggtgaatttt ttgttcaggt ctggggaaat ggagccaact
     2161 tcgacaacac gattttgcgc cgttcatacg aacggcaggg gatcccctgc ccgtggcgtt
     2221 actacaacga tcgcgatgta cgcacaatcg ttgagctggg gaaagccata gacttcgatg
     2281 ccagaacggc tattccattc gaaggtgagc gccataatgc acttgatgac gcccgttacc
     2341 aggcaaaata cgtttcagtt atctggcaaa aactgatccc gaatcaggct gatttttaat
     2401 gttcaacccc ggtcgttgcc caccagctat agtggcggcg accatgatta gcgaacgacg
     2461 ctcatggcaa gacttattct gctcactgag tgggcaaaag aggaattcag tgaaccggtc
     2521 ccaactccga gtacgttaag taaatacgct aaagccggaa tgatatttcc tctccccaaa
     2581 aaagttggaa gacgctggcg agtggatccg caagctcgct ttgtcggaat ggtaaacaag
     2641 ccggaggtga tcgccacaga tcaccctgct ttgaagagga tactggaaga tggcgcgccc
     2701 gcgaaaatat aaaaccgatg ttccgggatt atctccgtat tttgacaaaa gaaataacaa
     2761 agtttactgg cgttacaggc atcccataac aggcaaaaat cacggtctcg gcagtattga
     2821 ccagaaactg gcagaaacta ttgcagcaga agcgaacagc cgtcttgccc ggcagcaaat
     2881 ggaacaaatg ctcagtctgc aggaga
//
LOCUS       DBFIFI010000333         2886 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.335, whole genome shotgun sequence.
ACCESSION   DBFIFI010000333 DBFIFI010000000
VERSION     DBFIFI010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.335"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..413
                     /locus_tag="N7B39_004757"
     CDS             <1..413
                     /locus_tag="N7B39_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGU2424573.1"
                     /translation="ILKQRTKNIALPSHTHQQQNPPQEKTVVSIAVDPESPESFRKRP
                     KRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHN
                     ELHADPLAFEEKHGSQVDLIFRFLDHAFATGVLG"
     gene            426..659
                     /locus_tag="N7B39_004758"
                     /pseudo
     CDS             426..659
                     /locus_tag="N7B39_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
     gene            655..1956
                     /locus_tag="N7B39_004759"
                     /pseudo
     CDS             655..1956
                     /locus_tag="N7B39_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309554.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
     gene            2104..2286
                     /locus_tag="N7B39_004760"
     CDS             2104..2286
                     /locus_tag="N7B39_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HGU2424574.1"
                     /translation="MRFVQLILLYFCTVVCTLYLVSGGYKVIRNYIRKKIDAAAAEKI
                     SASQSAGTKPEEPLIS"
     gene            2324..2593
                     /locus_tag="N7B39_004761"
     CDS             2324..2593
                     /locus_tag="N7B39_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HGU2424575.1"
                     /translation="MSEIKSLVTAEAVKDVLRSEEVRSALKQQLRQNLEARLDAEVDA
                     ILDELLGGPAAPEPEDGAGDSAVSDGVVSQPDGSSEPQPGGEMMM"
     gene            2669..2884
                     /locus_tag="N7B39_004762"
     CDS             2669..2884
                     /locus_tag="N7B39_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HGU2424576.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
BASE COUNT          737 a          669 c          847 g          633 t
ORIGIN      
        1 gcatactgaa gcagcgcaca aaaaatattg cgctaccgtc tcacacccac cagcaacaga
       61 acccaccaca ggaaaagacg gtggtcagca ttgccgttga tccggagtct ccggaatcct
      121 tcaggaaacg acctaaacgt cgccgctggg taaatgagaa atacacacgc tgggtaaaga
      181 cacagccgtg tgcgtgttgt ggtaagccag cggacgatcc tcatcatctg attggtcatg
      241 gtcagggcgg aatgggaaca aaatcccacg atattttcac gctaccgctg tgtcgggagc
      301 atcacaacga gcttcatgcg gatccgctgg cgttcgaaga aaagcatggt tcccaggttg
      361 atttaatttt tcgttttctt gatcacgcct ttgcaaccgg cgtgctcggg taaaagaggt
      421 tactgatgcg tatagagttt gttttgcttt acccgccgac ggtgaacacc tactggcgac
      481 gtcgtggcag cacatatttt gtatcaaaag ccggtgagcg ttatcgccgg gctgtggcgc
      541 ttattgttcg ccagcagcgg ctgaaattaa gcctgtccgg aaggctggcg ataaagatta
      601 ttgccgagcc accggataag cgccgccgtg acctggacaa tattctgaaa gcgccgctgg
      661 ggtgtgggca ggccgctgta taaggatctc atcggtcgta caaaagccgc gctggcaaag
      721 aacccgaaaa atgtgctgct tgccgtggtg tggatgcagg gggaatttga ctttgacgga
      781 acgccagcaa atcacacagc ccgttttaca gaagtagtgg aacaatatcg tacggacctt
      841 gcagatatgg tgggacagtg cgctggtggt tctgctgacg gtgttccctg gatatgtgga
      901 gacacaactt atttctggaa gcagaagagc gaatccactt accagacggt gtacggcagt
      961 tacaaaaaca aaacggaaaa gaatattcac tttgtgccgt tcatgaccga tgagaacgga
     1021 gcaaatgtcc cgacgaacaa accggaagaa gacccggata ttccggcatc aggatattac
     1081 ggtgcggcct cccggacgtc ggcaaactgg acgtcagcag accgtgcgag ccatttcagc
     1141 tcatgggcac gcagggggat tatttctgac cgtcttgcct cagcgattct tctccatgca
     1201 ggacggacgg ctgaactggt gggtggggaa caggttgtga tgccgccgga tgagaagccg
     1261 tcaccggaca caccatcaac accgtcaacg gacgggaaat cagtgacaac gctgctttat
     1321 taccgtgcaa cagagtcagg tggtttactg aatccgcagg gatggggagc tgaaggaggg
     1381 cgtgcattgg tagttgatga tgcaggtgct gcaggaggta aggcgctgag gtggaccaaa
     1441 cagacaggaa gttcctcgtg gtttatgcag catgatgccg gtaatggcgc agacctgctg
     1501 gagaagggcg ggcttatcag ttgtcgtttt aaagttgatg gcacactgac agctaatcag
     1561 tacgcactgg cgctgtactg gccggtttct tcactgcctc agggtgtcac actggaaggt
     1621 aatgccggtc ataacctgct ggcgtcgttt tacgtacaga gcgatgccac agaccttaat
     1681 gtgatgtacc acaagggaaa tgctggtcag aacacgaagc tggggtcatt cggcgcattt
     1741 gataacgaat ggcatacgct gggcttccgt tttgccggta acaacagtat tgaggtgacg
     1801 ccggtcattg atggtaagga cgggacgccg ttcatgctgt cacagtcacc ggtcggcacg
     1861 tttacggcag acaaattgcg cgtgaccgat atcactagcg gtgcgacata tccggtgctg
     1921 attgaaagta taacagtgga agtgaataac ccgtaagcag gaaaaaaagg ccgccggggc
     1981 agggaaaaca aggagccaga accggcggca aatgtcgtta tatccaaagc aaaacatgca
     2041 ggacactttt ttaaccaaca ggtattaacg atgtcaacac catatcaata accgggaggg
     2101 ataatgagat ttgtacagct tattttattg tatttctgca cggtggtgtg cacgttatat
     2161 ctggtaagtg gcgggtataa ggttatccgg aactatatac gcaaaaagat tgatgccgcg
     2221 gcggcggaaa aaatcagcgc cagccagtca gccggaacaa aacccgaaga gcctctcatt
     2281 tcgtagcaac tttcttaaca acacctttca acgagaaaat cccatgtcag aaataaaatc
     2341 tctggtcact gctgaagcag tgaaggacgt cctgcgctct gaagaagtca gaagcgcact
     2401 gaaacagcaa ctccgccaga atcttgaggc gcgtcttgat gctgaagtgg atgccattct
     2461 ggatgagctg ctggggggac cggctgctcc tgagcctgaa gacggcgcgg gtgacagtgc
     2521 tgtttcagat ggcgttgtgt ctcagcctga cggtagcagt gagcctcagc ctggcggcga
     2581 aatgatgatg taaccatacg caggggctgt cggtgtgagc tgatgcccct cctgttgttg
     2641 tgagcttccg gattgcggga gacggggtat gtaccagatg gaaaaaatca caacgggtgt
     2701 gtcatacacc acgtcagcgg tggggacggg atactggtta ctgcagctgc tggacaaagt
     2761 ctctccgtcc cagtgggtgg cgataggtgt gctggggagt ctgctgtttg gcctgctgac
     2821 gtatctgact aacctgtatt tcaaaatcag agaggaccgt cgtaaggcgg cgcggggaga
     2881 gtaaag
//
LOCUS       DBFIFI010000334         2880 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.336, whole genome shotgun sequence.
ACCESSION   DBFIFI010000334 DBFIFI010000000
VERSION     DBFIFI010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.336"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(583..855)
                     /locus_tag="N7B39_004763"
     CDS             complement(583..855)
                     /locus_tag="N7B39_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HGU2424577.1"
                     /translation="MALKCPECGTTAHARTSAYEAPSVKRSWYQCQNLECSYTFTALE
                     SVDTIIMKPRRNEQESEKAQMPEKQQQTLNRYGSASKLSSRQQIPV"
     gene            complement(861..1412)
                     /locus_tag="N7B39_004764"
     CDS             complement(861..1412)
                     /locus_tag="N7B39_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage polarity suppression protein"
                     /protein_id="HGU2424578.1"
                     /translation="MMARVTPDQALISFRNARILWAGHSESRKAVEQQIESLLTAMEK
                     PADYARQLELQREHLDVLKWQINCAARECIYSQHQLMEACTEDALSNFMQANGAALTS
                     ALAPFLKGRGGVDVASRILRNALVRQLAITPPEIAGDYRMILDESGVMPDPMMVRDCQ
                     SSYTPAQQLRFQQRLDYINGMQE"
     gene            complement(1409..2161)
                     /locus_tag="N7B39_004765"
     CDS             complement(1409..2161)
                     /locus_tag="N7B39_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation initiation protein"
                     /protein_id="HGU2424579.1"
                     /translation="MTIPEQNNNPIVEISIPVSTQQALEKVNATKSAWLEARRQQKAA
                     ADNIATIRQRRAEMEATTNALNEEWRTLFRESQGVVSKEMKKLRTEIALGRETLEDFD
                     ELLAAQESENALLPQEAAELAGKYIHAHDTLVGIRAKQIWEDFMQSHGKALIQTLSLL
                     KSTMGREASAVVGVVHSVNDPDTLLKDFIHKHITRPALTNDAMPEQDPVFKLAGVAPD
                     YAARLDFSNKLSPAAMHKMKVRQERAEKEKAV"
BASE COUNT          690 a          663 c          661 g          866 t
ORIGIN      
        1 ttaatagcgt cagtctcagt gattcatttt atcacgcatt aatcaaaagg gactataatt
       61 tccagattgt aattcttgag aataaaggtc cgacgttcca aataaaaatt aatgatgcaa
      121 ataaaatcca taacttaaat aagaatggaa gctctggttt ttatcctgtt taatacatta
      181 aaaatataac tgctaattgg ggtgctcgaa attatatttc gtttcgagcg ctcagttcat
      241 ttctaaataa tttgcaaaat tattttgggt actttcgcct ctacacttcc ttcgggtggt
      301 tccacaataa tggatctcac cggcgtttga tattttataa tctaacccct tagcgcgcgc
      361 agccccccac gcctgctcgc ttcgcttaac agactggttt tcatgcattc cgcaaatcgt
      421 ctcagaagcc accacacaag ggctttcgcg tcaaaaatgg tgcatgagaa tcatgcgttt
      481 tcatgcgcta tagacatgca ctcatgcgct ctcaggccag ccagggaaaa ggcgtaaaaa
      541 atcccagtac tggaccggga ttacgtgggc tttttttgct aatcagacag gaatttgctg
      601 acggcttgac agctttgacg cggagccata gcgattgaga gtttgctgtt gtttttccgg
      661 catttgtgct ttctctgatt cctgttcatt gcgccggggc ttcatgatta tcgtatccac
      721 actttccagt gcggtaaatg tgtaggaaca ttcaagattc tggcactgat accatgagcg
      781 tttaaccgat ggtgcttcat aggcggaggt tctggcgtgt gccgtcgtac cgcattcggg
      841 acatttaagt gccattaata ttactcctgc atgccgttaa tgtaatccag acgttgctga
      901 aagcgcagtt gttgtgcagg ggtgtaagag ctctggcaat cacgaaccat catggggtca
      961 ggcatcacac cggattcatc cagaatcata cgataatccc cggcaatttc aggcggtgtt
     1021 attgccagct ggcgaaccag agcattgcgt aatatacggg acgcaacgtc aacccctccg
     1081 cgccccttca ggaacggagc cagtgcagat gtcagggctg ctccattcgc ctgcataaaa
     1141 ttgctgagag cgtcttctgt gcaggcctcc atcagttgat gctgcgagta aatgcattcg
     1201 cgcgcagcac agttaatttg ccatttcagc acatccagat gttcccgctg taattcgagc
     1261 tgtcgagcat aatctgctgg tttttccatg gcagtgagga gtgattctat ctgttgttca
     1321 acggcttttc ggctctcact atgtccagcc cacagaatac gggcatttct aaaactgata
     1381 agcgcttggt ctggagtgac tcgggccatc atactgcttt ctccttctca gcacgttcct
     1441 gacgaacctt cattttatgc atagctgctg gagagagttt attactgaaa tccagacggg
     1501 ccgcataatc cggggcaact cccgccagtt taaacacggg gtcctgttcg ggcatcgcat
     1561 cgttagtcag agccggtctg gtgatatgtt tatgaataaa atctttcagc agtgtgtccg
     1621 ggtcattaac tgaatgcacc acaccgacaa ctgcgctggc ttccctgccc attgttgatt
     1681 tgagcaggct tagtgtctgg attagtgctt ttccatgcga ctgcataaaa tcttcccaga
     1741 tttgttttgc gcgaataccc acaagggtgt cgtgcgcgtg gatatacttt ccggctaatt
     1801 ccgcagcctc ctgcggcaaa agtgcatttt cgctttcctg agccgccagc agctcatcaa
     1861 aatcctcaag cgtttctcgt cccagcgcaa tttccgtgcg cagttttttc atttcctttg
     1921 agactacgcc ctgactttca cgaaatagcg tgcgccactc ctcattcagg gcgttcgtcg
     1981 tggcttccat ttcagcgcgg cgctggcgga ttgtcgcaat attatccgct gccgcttttt
     2041 gctgacgacg tgcttcaagc catgcagatt tggtggcatt cactttctca agcgcctgtt
     2101 gcgtcgaaac agggatagaa atttcaacga tcgggttatt gttctgttct gggattgtca
     2161 tgtcagcacc tagtggtttg tcgtggtgtc aattgtgctg tgatgcatac aaagctgcca
     2221 ttgaacgcca ttgtgcgagc gacaagacaa agactgaacc gccccggacc tcttttggct
     2281 agccagaaaa ggcctcgttt aactaaggct gtttcagact taccttaact attcactact
     2341 attcacttag gtaaaaaaaa taggtaatac agaaggttat aaggtgaagg gttggcatgg
     2401 cagtattcat cgactgttca taactgttca catgggaata aaattattta tggtattttt
     2461 tcataatggg tggttttttt attcctcgta gctatttgtt ttttgctcct caccactatt
     2521 cacccttatt caggagtatt caacaggcac ataaatagac tgcatgtttt tgccggggaa
     2581 taacattttc gcctctggcc atggtgttgc atttcatact gatttttata aaactagatc
     2641 tgtccggttc tttatggatt cattcggata tattttacga tttaaggtga tgttatttaa
     2701 tatcaaggtt tttatgcagg cccaatgttt ataagttgga tgcgattcaa aaatatgccc
     2761 ggaaattggg gcctgcaatg caaggcctcg tgtagaagtt gtactgcctt gctgtattgg
     2821 cggtggggtt agcactatca ggtgcaatga cccataggat agtaaaccct gggtgaatgg
//
LOCUS       DBFIFI010000335         2859 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.337, whole genome shotgun sequence.
ACCESSION   DBFIFI010000335 DBFIFI010000000
VERSION     DBFIFI010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.337"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            25..1230
                     /gene="yedE"
                     /locus_tag="N7B39_004766"
     CDS             25..1230
                     /gene="yedE"
                     /locus_tag="N7B39_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HGU2424580.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVAAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            1227..1460
                     /gene="yedF"
                     /locus_tag="N7B39_004767"
     CDS             1227..1460
                     /gene="yedF"
                     /locus_tag="N7B39_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HGU2424581.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            1569..2237
                     /locus_tag="N7B39_004768"
     CDS             1569..2237
                     /locus_tag="N7B39_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HGU2424582.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAREWMRQEISGKEASEIAASGCVPANQFSWHPVSRAVGNVKNQGAEL
                     IQPV"
     gene            2348..2827
                     /locus_tag="N7B39_004769"
     CDS             2348..2827
                     /locus_tag="N7B39_004769"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU2424583.1"
                     /translation="MFTIKTDDLTHPAVQALVAYHISGMLQQSPPESSHALDVQKLRN
                     PTVTFWSVWEGEQLAGIGALKLLDDKHGELKSMRTAPNYLRRGVASLILRHILQVAHD
                     RCLHRLSLETGTQAGFTACHQLYLKHGFVDCEPFADYQLDPHSRFLSLTLCEDNELL"
BASE COUNT          640 a          708 c          762 g          749 t
ORIGIN      
        1 cttctgagtt cagaggctac attcatgtca tggcagcaat tcaaacacgc ctggttgatt
       61 aaattctggg cgcccatccc cgcggtcatc gcggcgggta ttctctccac ttactatttt
      121 ggcatcactg gcaccttttg ggctgtcacg ggtgaattta cccgttgggg cggtcagctc
      181 ctgcagctgt tcggcgtcca tgctgaagag tggggttatt ttaaaattat ccatctggaa
      241 ggatcgccat taacccgcat cgacgggatg atgatcctcg gtatgtttgg cggctgcttt
      301 gccgcagcgc tgtgggccaa caatgtcaaa ctacgcatgc cgcgtagccg tatccgcatt
      361 atgcaggcca ttattggcgg cattatcgcc ggttttggcg cgcgtctggc aatgggctgt
      421 aacctggcgg cattctttac cggtattcct cagttctcgc tgcatgcctg gttctttgcc
      481 atcgccactg ccattggttc atggtttggc gcgcgcttta ccctgctgcc catcttccgt
      541 attcccgtga aaatgcagaa agttgctgcc gcctcaccgc tgacgcaaaa accggatcag
      601 gcgcggcgtc gttttcgtct cgggatgctg gtctttttcg gcatgctggg ctgggcgctg
      661 ctcacagcga tgaaccaacc caaactgggg ctggcaatgc tgtttggcgt cggctttggt
      721 ttactgattg aacgtgcgca aatctgcttt acttcggcgt tccgcgatat gtggatcacc
      781 ggacgtaccc atatggcgaa agcaatcatt attggtatgg cggtgagtgc catcgggatc
      841 ttcagttacg tacagttagg cgttgaaccc aaaatcatgt gggcgggacc aaacgcggta
      901 attggtggtt tactgtttgg ttttggcatc gtgctggctg gcggctgcga aaccggctgg
      961 atgtaccgcg cggtagaagg ccaggtgcac tactggtggg tcggtctggg caatgtgatc
     1021 ggctcaacga ttctggcgta ttactgggat gatttcgctc cggcgctggc caccgactgg
     1081 gacaaaatca acttgctgaa aacctttggt ccgatggggg gcctgctggt gacatatttg
     1141 ctgttgtttg ctgcgctgat gttgattatc ggctgggaaa aacgcttctt ccgccgtgcg
     1201 gcaccgcaga ctgctaagga gatcgcatga aaaatatcgt tcctgattac cgccttgata
     1261 tggtgggtga accctgccct tatccggcag tcgcaaccct tgaggcgatg ccgcagttaa
     1321 aaaaagggga aatcctggaa gtggtgagcg actgtccgca gtcgatcaat aatattccac
     1381 tggatgcgcg taatcacggc tatacggtgc tggatattca gcaagacggg ccgaccattc
     1441 gttatttaat tcagaagtaa tccctcattc ccgctgtgac ccacagcggg aacatcgtta
     1501 cctttgtatc acatagattt tacccgcccc ctctcctatt cttttcagta tgatctcagg
     1561 aggtttctat gtgtggacgc tttgcccaat cccaaacgcg tgaagattac cttgcgcttc
     1621 tcgcggaaga tattgaacgc gatattccct atgatcccga acccattggc agatacaacg
     1681 tcgcgccggg aaccaaagtc ctactgctca gtgaacgtga tgaacacctt catctggatc
     1741 cggttttctg gggatatgct cccggatggt gggataaacc gccgctgatt aacgcccgcg
     1801 tagaaactgc ggcaaccagt cgtatgttta aaccgctctg gcaacatggt cgggcaatct
     1861 gttttgccga tggctggttt gagtggaaaa aagaaggcga caaaaagcag ccttatttta
     1921 tctatcgtgc tgatggacaa cctgttttta tggcagcgat aggcagcaca ccgttcgagc
     1981 gcggtgacga agccgaagga tttttgattg tcactgcagc agcagaccag ggtctggtag
     2041 atattcatga ccgccgcccg ctggttctgt cgccagaagc tgcgcgggaa tggatgcggc
     2101 aagagattag cggtaaagaa gcctcagaaa tcgcggccag tggctgtgtt ccagcaaacc
     2161 agttctcctg gcacccggta tcgcgcgcgg tgggtaatgt taaaaaccag ggtgcggagt
     2221 taattcaacc tgtttgataa gcctggagat tattgatcaa caatactgcg tcataagtaa
     2281 tctctattag acaaagattt cattacctgt tggcatattg caaaaataac accaatacgg
     2341 aatcgtcatg ttcacgatta aaacagatga tctcacccat ccagcagtgc aagcattagt
     2401 ggcttaccat atttccggca tgctgcagca gtctccccct gaaagcagtc atgctttaga
     2461 cgtgcaaaaa ttacgtaacc cgacagtgac attctggtca gtatgggaag gcgaacaact
     2521 cgcaggaatt ggtgcgctga agttgctgga tgataaacat ggcgaactga aatcaatgcg
     2581 gaccgcgcca aattatttac gtcggggtgt cgccagtctg attttacgcc acattttgca
     2641 ggtcgcccat gacagatgcc ttcatcgcct gagtttagaa acgggtacac aggctggatt
     2701 tacggcctgc catcaacttt atttgaagca tggtttcgtt gattgcgaac cgtttgccga
     2761 ttatcaactt gatccacaca gtcgattttt gtcattgacg ctatgcgaag ataatgagtt
     2821 gctttgagcc agacgcagca cattcttgca ttcgacgtg
//
LOCUS       DBFIFI010000336         2794 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.338, whole genome shotgun sequence.
ACCESSION   DBFIFI010000336 DBFIFI010000000
VERSION     DBFIFI010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.338"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..147
                     /locus_tag="N7B39_004770"
     CDS             <1..147
                     /locus_tag="N7B39_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312523.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WlaTC/HtrL family glycosyltransferase"
                     /protein_id="HGU2424584.1"
                     /translation="FVMCYYKRPDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQALRIF
                     LSRK"
     gene            422..1618
                     /gene="kbl"
                     /locus_tag="N7B39_004771"
     CDS             422..1618
                     /gene="kbl"
                     /locus_tag="N7B39_004771"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HGU2424585.1"
                     /translation="MRGEFYQQLTNDLETARAEGVFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            1628..2653
                     /gene="tdh"
                     /locus_tag="N7B39_004772"
     CDS             1628..2653
                     /gene="tdh"
                     /locus_tag="N7B39_004772"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HGU2424586.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMCSGQSG
                     KVILSWD"
BASE COUNT          646 a          642 c          773 g          733 t
ORIGIN      
        1 tttgtcatgt gctattacaa acgccccgat ctcttcaacc taaactatct ggggagagga
       61 aaatggttcg atttgtttcg ctgcttcagg agtaacactt taggggcaaa aatgcaggcg
      121 ctgagaattt tcctttcaag aaaataggtt tccctccatt agtgggtagc gttttgatac
      181 caactgagat aggaaaaatg atgctttggc atcatttttt ctttttatat caatgcagta
      241 aaatcaatca tcattatgcc cagccaaaac tgtacagttt ttctactatc tgtaggtgtt
      301 gagagacatc aagttacacg tcatttatcc tgaattttgc agaagagtta acgcgtcatc
      361 tcgtcgcgac ctataagttt gggtaatatg tgctggaatt tgccctgtct ggagaatcgc
      421 aatgcgtgga gaattttatc agcagttaac caacgatctg gaaaccgcac gggcggaagg
      481 ggtgtttaaa gaagagcgca ttattacgtc tgcgcagcaa gcagatatca ctgtggctga
      541 tggaagccac gtcattaact tttgtgccaa caactatctc gggctggcga atcatccgga
      601 tctgattgcg gcagcaaagg cgggaatgga ttctcacggt ttcggcatgg cttcggtgcg
      661 ttttatttgc ggcactcagg acagccataa agagcttgaa caaaaactgg cggcattcct
      721 ggggatggaa gatgcgattc tctactcttc ctgctttgat gctaacggtg gactgtttga
      781 aacgctgctg ggtgcggaag acgccattat ctccgacgca ctgaaccacg cgtctattat
      841 tgatggtgtg cgtctgtgca aagctaaacg ctatcgctat gccaacaacg atatgcagga
      901 gctggaagca cgtctgaaag aagcgcgtga agccggtgcg cgtcatgtgc tgattgccac
      961 cgatggtgtg ttctcaatgg acggcgtgat tgccaacctg aaaggcgttt gcgatctggc
     1021 agataaatac gatgccctgg tgatggtgga tgactcccac gcggtcggtt ttgtcggtga
     1081 aaatggtcgt ggttcccatg aatactgcga tgtgatgggc cgggtcgata ttatcaccgg
     1141 tacgcttggt aaagcgctgg gcggggcttc tggtggttat accgcggcgc gcaaagaggt
     1201 ggttgagtgg ctgcgccagc gttctcgtcc gtacctgttc tccaactcgc tggcaccggc
     1261 cattgttgcc gcgtccatca aagtactgga gatggtcgaa gcgggcagcg aactgcgtga
     1321 ccgtctgtgg gcgaacgcgc gccagttccg tgagcaaatg tcggcggcgg gctttaccct
     1381 ggcgggagcc gatcatgcca ttattccggt catgcttggc gatgcagtag tggcgcagaa
     1441 atttgcccgt gagctgcaaa aagagggcat ttacgtcacc ggtttcttct atccggtcgt
     1501 tccgaaaggt caggcgcgta ttcgtaccca gatgtctgcg gcgcataccc ctgagcaaat
     1561 tacgcgtgca gtagaagcat tcacgcgtat tggtaaacaa ctgggcgtta tcgcctgagg
     1621 atgtgagatg aaagcgttat ccaaactgaa agcggaagag ggcatctgga tgaccgacgt
     1681 tcctgtaccg gaactcgggc ataacgatct gctgattaaa atccgtaaaa cagccatctg
     1741 cgggactgac gttcacatct ataactggga tgagtggtcg caaaaaacca tcccggtgcc
     1801 gatggtcgta ggccatgaat atgtcggtga agtggtaggt attggtcagg aagtgaaagg
     1861 cttcaaaatc ggcgatcgcg tttctggcga aggccatatc acctgcggtc actgtcgcaa
     1921 ctgtcgtggc ggtcgtaccc atctgtgccg caacaccatt ggtgtcggtg ttaaccgtcc
     1981 gggctgcttc gccgaatatc tggtgattcc ggcgttcaac gctttcaaaa tccccgacaa
     2041 tatctccgac gacctggctt ccatttttga tcccttcggt aacgccgtgc ataccgcgct
     2101 gtcgttcgat ctggtgggcg aagatgtgct ggtttctggt gcaggtccga taggtattat
     2161 ggctgcggcg gtggcgaaac acgttggtgc acgcaatgtg gtgatcaccg atgttaacga
     2221 ataccgcctt gagctggcgc gcaaaatggg tatcacccgt gcggttaacg tcgcgaaaga
     2281 aaaccttaat gatgtgatgg ctgaactggg catgaccgaa ggctttgatg tcggtctgga
     2341 aatgtccggc gcgccgccag cgtttcgtac catgctcgat accatgaacc acggcggtcg
     2401 tattgcgatg ctgggtattc caccgtctga catgtctatc gactggacca aagtgatctt
     2461 taaaggcttg ttcattaaag gcatttacgg tcgtgagatg tttgaaacct ggtacaagat
     2521 ggcggcgctg attcagtctg gcctcgatct ctcgccgatc attacccatc gtttctctat
     2581 cgatgatttc cagaagggct ttgacgctat gtgttcgggc cagtccggga aagttattct
     2641 gagctgggat taacacgaaa aagggctggt attccagccc ttttgcctga ggataatctg
     2701 ttaaatatgt aaaatcctgt cagtgtaata aagagttcgt aattgcgctg atcttttata
     2761 tagctgttct cattatctct ctaccctgaa gtga
//
LOCUS       DBFIFI010000337         2781 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.339, whole genome shotgun sequence.
ACCESSION   DBFIFI010000337 DBFIFI010000000
VERSION     DBFIFI010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.339"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            104..715
                     /gene="map"
                     /locus_tag="N7B39_004773"
     CDS             104..715
                     /gene="map"
                     /locus_tag="N7B39_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector Map (Rho
                     guanine exchange factor)"
                     /protein_id="HGU2424587.1"
                     /translation="MFSPMTMAGRSLVQATAQTLKPAVTRAAMQAGTGATGMRFMPVQ
                     SNFVINHGKLTNQLLQAVAKQTRNGDTQQWFQQEQTTYISRTVNRTLDDYCRSNNSVI
                     SKETKGHIFRAVENALQQPLDMNGAQSSIGHFLQSNKYFNQKVDEQCGKRVDPITRFN
                     TQTKMIEQVSQEIFERNFSGFKVSEIKAITQNAILEHVQDTRL"
     gene            1139..>2781
                     /gene="tir"
                     /locus_tag="N7B39_004774"
     CDS             1139..>2781
                     /gene="tir"
                     /locus_tag="N7B39_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocated
                     intimin receptor Tir"
                     /protein_id="HGU2424588.1"
                     /translation="MPIGNLGHNPNVNNSIPPAPPLPSQTDGAGGRGQLINSTGPLGS
                     RALFTPVRNSMADSGDNRASDVPGLPVNPMRLAASEITLNDGFEVLHDHGPLDTLNRQ
                     IGSSVFRVETQEDGKHIAVGQRNGVETSVVLSDQEYARLQSIDPEGKDKFVFTGGRGG
                     AGHAMVTVASDITEARQRILELLEPKGTGESKGAGESKGVGELRESNSGAENTTETQT
                     STSTSSLRSDPKLWLALGTVATGLIGLAATGIVQALALTPEPDSPTTTDPDAAASATE
                     TATRDQLTKEAFQNPDNQKVNIDELGNAIPSGVLKDDVVANIEEQAKAAGEEAKQQAI
                     ENNAQAQKKYDEQQAKRQEELKVSSGAGYGLSGALILGGGIGVAVTAALHRKNQPVEQ
                     TTTTTTTTTTTSARTVENKPANNTPAQGNVDTPGSEDTMESRRSSMASTSSTFFDTSS
                     IGTVQNPYADVKTSLHDSQVPTSNSNTSVQNMGNTDSVVYSTIQHPPRDTTDNGARLL
                     GNPSAGIQSTYARLALSGGLRHDMGGLTGGSNSAVNTSN"
BASE COUNT          827 a          534 c          666 g          754 t
ORIGIN      
        1 aggataattc aaatataaat acaagataat tattctttaa agttattttt taatgggaag
       61 caattttttt actgtcgaaa tataatttat aaaggtttat ggtatgttta gtccaatgac
      121 aatggcaggc agatcgttag ttcaggcgac tgcacagacg cttaaacctg cggttaccag
      181 agctgcaatg caagcgggta cgggagccac gggtatgagg ttcatgcccg tgcagtcgaa
      241 ctttgtgatt aatcatggca aactgactaa ccaactcctg caggctgtag ccaaacaaac
      301 tcgtaatggc gatacccaac aatggttcca gcaggagcag acgacttata tatccagaac
      361 agtaaataga actctggatg actattgcag gagtaataat tcggtcatta gtaaggagac
      421 taaaggtcac atttttagag ctgttgaaaa tgcgttacag caacctcttg atatgaacgg
      481 ggcacaatca tcaattggtc acttcttgca gtccaataaa tactttaatc agaaagttga
      541 tgagcagtgc ggcaaaagag tggatccaat aacacgtttt aacacccaga cgaagatgat
      601 agaacaagtc tcacaagaaa tatttgaacg caactttagc ggcttcaaag tcagtgagat
      661 aaaagcgatt actcagaatg cgattttaga gcatgtacag gatacccgat tgtagggata
      721 accttgtcag gtataaatta agcttcctgg tgtatagcat ggcctttgtt gatgtaagaa
      781 aaaacgcgat aaagaaactt aataaacttt attattaatt aagatttata gtttgcttaa
      841 ttggttttct ttggctgtgt tttttaatat aaatgaatgt ttgctgcaat tttatttatg
      901 aaatgtttta ttaagatttc acgtggtgtt tacttttata taatataatt atttttattt
      961 tgttacccga tgtgtgtttt tagcacatta ttttcttgca tcaaaaatca gattgtgatt
     1021 tatttggtcg atgtcatgtt cagccgttta tcgactacgt gctgtatacc atacattatt
     1081 atgtaatagc aaaataaagg attgttatct atttacataa aataaaaagg agatatttat
     1141 gcctattggt aatcttggtc ataatcccaa tgtgaataat tcaattcctc ctgcacctcc
     1201 attaccttca caaaccgacg gtgcaggggg gcgtggtcag ctcattaact ctacggggcc
     1261 gttgggatct cgtgcgctat ttacgcctgt aaggaattct atggctgatt ctggcgacaa
     1321 tcgtgccagt gatgttcctg gacttcctgt aaatccgatg cgcctggcgg cgtctgagat
     1381 aacactgaat gatggatttg aagttcttca tgatcatggt ccgctcgata ctcttaacag
     1441 gcagattggc tcttcggtat ttcgagttga aactcaggaa gatggtaaac atattgctgt
     1501 cggtcagagg aatggtgttg agacctctgt tgttttaagt gatcaagagt acgctcgctt
     1561 gcagtccatt gatcctgaag gtaaagacaa atttgtattt actggaggcc gtggtggtgc
     1621 tgggcatgct atggtcaccg ttgcttcaga tatcacggaa gcccgccaaa ggatactgga
     1681 gctgttagag cccaaaggga ccggggagtc caaaggtgct ggggagtcaa aaggcgttgg
     1741 ggagttgagg gagtcaaata gcggtgcgga aaacaccaca gaaactcaga cctcaacctc
     1801 aacttccagc cttcgttcag atcctaaact ttggttggcg ttggggactg ttgctacagg
     1861 tctgataggg ttggcggcga cgggtattgt acaggcgctt gcattgacgc cggagccgga
     1921 tagcccaacc acgaccgacc ctgatgcagc tgcaagtgca actgaaactg cgacaagaga
     1981 tcagttaacg aaagaagcgt tccagaaccc agataatcaa aaagttaata tcgatgagct
     2041 cggaaatgcg attccgtcag gggtattgaa agatgatgtt gttgcgaata tagaagagca
     2101 ggctaaagca gcaggcgaag aggccaaaca gcaagccatt gaaaataatg ctcaggcgca
     2161 aaaaaaatat gatgaacaac aagctaaacg ccaggaggag ctgaaagttt catcgggggc
     2221 tggctacggt cttagtggcg cattgattct tggtggggga attggtgttg ccgtcaccgc
     2281 tgcgcttcat cgaaaaaatc agccggtaga acaaacaaca acaacaacta ctacaactac
     2341 aactacaagc gcacgtacgg tagagaataa gcctgcaaat aatacacctg cacagggcaa
     2401 tgtagatacc cctgggtcag aagataccat ggagagcaga cgtagctcga tggctagcac
     2461 ctcgtcgact ttctttgaca cttccagcat agggaccgtg cagaatccgt atgctgatgt
     2521 taaaacatcg ctgcatgatt cgcaggtgcc gacttctaat tctaatacgt ctgttcagaa
     2581 tatggggaat acagattctg ttgtatatag caccattcaa catcctcccc gggatactac
     2641 tgataacggc gcacggttat taggaaatcc aagtgcgggg attcaaagca cttatgcgcg
     2701 tctggcgcta agtggtggat tacgccatga catgggagga ttaacggggg ggagtaatag
     2761 cgctgtgaat acttcgaata a
//
LOCUS       DBFIFI010000338         2781 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.340, whole genome shotgun sequence.
ACCESSION   DBFIFI010000338 DBFIFI010000000
VERSION     DBFIFI010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.340"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(39..785)
                     /gene="traX"
                     /locus_tag="N7B39_004775"
     CDS             complement(39..785)
                     /gene="traX"
                     /locus_tag="N7B39_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HGU2424589.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSLLVWSPGQRDIIKTVALVLM
                     VLDHANRILHLDQTWMFLAGRGAFPLFALVWGLNLSRHAHIRQSAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSHRLYRAEDRTERLVLVACLLAVIPALNLASSDAAAVAGLVMTVLTVGLVSC
                     AGKSLPRFWPGDFFPTFYGCHLAVLGILAL"
     gene            complement(805..2193)
                     /locus_tag="N7B39_004776"
                     /pseudo
     CDS             complement(805..2193)
                     /locus_tag="N7B39_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI
                     domain-containing protein"
     gene            2250..2501
                     /locus_tag="N7B39_004777"
                     /pseudo
     CDS             2250..2501
                     /locus_tag="N7B39_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2508..2753
                     /locus_tag="N7B39_004778"
                     /pseudo
     CDS             2508..2753
                     /locus_tag="N7B39_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          586 a          910 c          713 g          572 t
ORIGIN      
        1 tttattaata agcagaatca gcgtcagtat cataccagtc acaacgccag aatgcccagc
       61 acagccagat gacagccata gaacgtcggg aaaaaatctc cgggccagaa acggggtaat
      121 gatttccctg cacaggacac cagaccaacg gtcagcaccg tcatcacaag accagctaca
      181 gccgccgcat cactggaggc aaggttaagt gccggaatca cggccagcag gcatgcaacc
      241 agcaccagac gttccgttct gtcttccgcc cggtacagcc ggtggctgac tgccagcatc
      301 agcagcccgg caatgccgta actggtgccg gacaaaggcc cccacagtgc catcaggaga
      361 atggccgctg ctgtacgcca cccactgcgc gtttcacacc acgtcagcac ctgtgccgcc
      421 actgcaaagg caaacaggat attcccctca taccacggaa agcctgccag ataatacgcg
      481 aactgggcaa taattcccca tccccacagc cggttaatgg ccgactgcct gatatgcgca
      541 tggcgggaca gattcagccc ccacaccaga gcaaacaacg gaaaggctcc acgcccggcc
      601 agaaacatcc acgtctggtc cagatgcaga atgcggtttg cgtggtccag taccatcagc
      661 accagtgcca ccgttttgat gatatcccgc tgtccgggtg accagaccag caacgactgc
      721 aaccaggtat cggtccgggc agccagtgaa tcgttacgtg tcgtgttcgt gttatccgtt
      781 gtcatcagtg tttgtcttcc tgtatcagtc tccgcccagg gttttctctt tctgcaggtc
      841 acgaaccata tcccgctcca tctgctgcag tcgctcctgc agcaggtttt cccggacaac
      901 ctcacggacc gcctcccgct cccgttgtgg ttcacgcagt acactctccg gcagcgcggc
      961 ttcccgttca gaagtgacag cccgttcacg ctcctgcccg gcaatctccc tgacagcctg
     1021 ctctgttctg ccgtccggca ggtcaggttt gttctctgcc attttacgga caatttcatc
     1081 tgcgcggcgt tccgtttcac ggcggatggc ctcttcagcc tgccgctgtg ccagtacctc
     1141 tgccgtgacc ggctcgccat ttccggctcc cggctggacg ctgttgtccg ggatatcccc
     1201 ccacacgcga ccaccggtga tggcaccggg attccacgga cgaccttcac cggcgattct
     1261 caccaccaca ccgctgtcag gattatcacg ggcaatccgg acaccatcct gcatattatc
     1321 agccagcagg ctctctccgt tacggctgcc ctgcagggcc acgaactgcg catccccgct
     1381 gcctttcacc cgtccttcac cactgaatcc ccgcagcccg tttccgtcat ccgtggtcag
     1441 cgggttcagc cagataccgg cagacttgcc gttacggtca aacgccggca gtgccacata
     1501 cggctgtgga tatttacgtc ccggagcaat aaaccgtgca ggactgtctc ccccggcaag
     1561 ccccgcctga cgaagaacgg cacgccctgc cgccacgtcc cgcagctccc gcgccgtact
     1621 gaacagccgc tctgcattca tgacctcccg gtccggtttc ggttcaaaca catcgtgggc
     1681 tgttcctttc tgtacggcat tgttaatggc atccgtccag ccctgacggt tatcggtgta
     1741 cacctgcaca tgctgcttca tacgcgacag ggccacgtag gctgactcaa agccggccat
     1801 cagtttccgg ctgccttccg taccttcaag cgcgatggca aaggtttcac ttgccccctg
     1861 cgcaccgtga gcggtgatgg cataggccag gtcaatatgt tgctctgccc gctcctgggc
     1921 ggggcgaatc acccgggtct gctgtccgcc cgacagcgtg acactgtcac cggaaaccgc
     1981 tgtcaccgtc cagacgctgt tggccacata accacgcccc cggtcactct tcgtgaagcg
     2041 catccggtca ccggtcccca ccctgatggt gtccgggggg tacagtgtga cgccttcagc
     2101 caccgcctcc cggggcgaaa tcagccgcgt gttaccttcc gcatcctgca gagttatcag
     2161 cccgtcatcc ctgctgatac cggcaatccg gtgattgaac cgccccggaa atcctggaga
     2221 ctaaactccc tgagaaagag gtaaacagga tgactaaaaa tactcgtttt tcccccgagg
     2281 tccgtcaacg ggcagttcgt atggttctgg aaagtcaggg cgaatatgac tcacaatggg
     2341 cggcaatttg ttccattgcc ccaaagattg gctgtacacc agagactctg cgtgtgtggg
     2401 ttcgtcagca tgagcgggat accgggagtg gtgatggtgg actcaccacc gctgaacgtc
     2461 agcgtctgaa agagctggaa cgtgaaaatc gtgaactgcg cgttcagttc ttccggctac
     2521 tgaaccgtcg atatgaaaaa gcgagcatca tactgacgtc aaacaaaggg ttcgcagact
     2581 ggggagaaat gttcggagag catgtgctgg caacagcgat cctggaccgg ctgctacatc
     2641 actcaaccac gctgaatatc aaaggagaga gttaccggtt caatattcca gacagactcg
     2701 tggttcattt gagcctccat gctcgccctg aactccgggc acacaatacg tgatgtctcc
     2761 cgcccacttc tggttcgggc c
//
LOCUS       DBFIFI010000339         2762 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.341, whole genome shotgun sequence.
ACCESSION   DBFIFI010000339 DBFIFI010000000
VERSION     DBFIFI010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.341"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(470..643)
                     /locus_tag="N7B39_004779"
     CDS             complement(470..643)
                     /locus_tag="N7B39_004779"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424590.1"
                     /translation="MNKTANVAAHAVVNAALAVVQGNNARASAAGAATGDVVGMIATE
                     MCGISPGTVTCKR"
     gene            821..1024
                     /locus_tag="N7B39_004780"
     CDS             821..1024
                     /locus_tag="N7B39_004780"
                     /inference="COORDINATES: protein motif:HMM:NF026057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase DNA-binding-containing protein"
                     /protein_id="HGU2424591.1"
                     /translation="MVLSDCYSLANEQSGHARLGDPRRTRRLVSLTSSLAQHAGLSIV
                     KSSHFTAQVEGAYRLIRNPSVSP"
     gene            complement(956..1429)
                     /locus_tag="N7B39_004781"
     CDS             complement(956..1429)
                     /locus_tag="N7B39_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001502749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424592.1"
                     /translation="MQVMPDIRFPVTDSDYLFLYLPQPFGHALRSLLPAVTFFFFTGT
                     FFSLVLNTVGVRGTTPALLFDKIDWQGAVGGVHQDRVSQQPVRLRVSGGIPQVIKCRT
                     VTEVNGGGIFQREEQRMCLTCPCSGSKSGFGNGLRGHRRVTYQAVRTFNLSSEVG"
     gene            1349..2089
                     /locus_tag="N7B39_004782"
     CDS             1349..2089
                     /locus_tag="N7B39_004782"
                     /inference="COORDINATES: protein motif:HMM:NF033590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HGU2424593.1"
                     /translation="MAERLREIQKQVITVCDREADIWHYLHYKVSRGKPHAECDAKRR
                     AGSPPGPDAHQLQRSQHKKSRQQRPGALAHVCLLRGAGEDGACWYPLTSEKVASAADA
                     RRIVSHYEQRWLIEEYHKAWKSGGTCVESLRMQTRDNLEHMVVIAVRVLGLRQGGISE
                     ETQNDSCEKILTPTEWKLLWVKLEGKQLPAQTPTLKWACLKLAKLGRWHDSKRTSSPG
                     WVVMWDGWFRHQDMAEGYLVMKSLDQEI"
     gene            2323..2730
                     /locus_tag="N7B39_004783"
                     /pseudo
     CDS             2323..2730
                     /locus_tag="N7B39_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152817.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
BASE COUNT          738 a          687 c          646 g          691 t
ORIGIN      
        1 atcatcaaaa aactataaat atatcacctg acgatttgaa cacactgaca aaaaaccaag
       61 catggtaata aaccgaactc cccacaaact tctgttgcct cagatactcc cccccctttc
      121 ctcaggcaca aatgttgttc cataacgaac cagccctgtt cctttaaact ctgcatcaat
      181 ggcataatgt atactcacca gactgagttt gctcctgcat cattatttcg atacgttaat
      241 gcattgttca ttgcatccgg cattccccat cgaatattta ctttgttata tcagtcagcc
      301 cataaccagg taaattttta aggcttactg actgaatatt caaaatgtgt tacatgttat
      361 caaaccttta aagaataacc tgctcttctt cattggccgg atttagctca atcttgtcag
      421 caaaactaat ataaagccgt tcataaagag aaggatgtag cttcgttatt tatcttttac
      481 atgtaacagt gccaggggat attccgcaca tctctgtggc tatcatcccc accacgtctc
      541 cagtggcagc acctgcggca ctggcacggg cgttatttcc ctgtaccacc gccagtgcag
      601 cattcaccac ggcatgagct gccacatttg cagtcttatt caccacacct gtaacctgtc
      661 tcttatacac aaatccccac ccggggattt gtgatgtctg tcagtctgct ttagggggac
      721 tctttccatc cggtagtttc cttcctcccc ggcagcgatt ttttgaactt tttcttcacc
      781 cgttgatctt ctcttatatt tcattttctt cgggtctgtt atggtacttt ctgattgtta
      841 ttcactggct aacgagcagt ctggacatgc ccgtctcggt gatccgcgtc gtacacgacg
      901 ccttgtgtca ctgacctctt ctctcgctca gcatgcaggt ctttctattg tgaaatcatc
      961 ccacttcact gctcaggttg aaggtgcgta ccgcctgata cgtaacccgt cggtgtcccc
     1021 gtaagccatt gccgaagccg gatttactgc cactgcaagg gcatgtgagg cacatccgct
     1081 gctcctcgcg ctggaagata ccaccaccat taacttcggt cacagtacgg catttgatga
     1141 cctggggaat accaccacta acccgaagac gcacgggctg ctggctcact ctgtcctgat
     1201 gtacgccccc gacagcgccc tgccagtcga tcttatcgaa cagcagtgct ggagtcgtgc
     1261 cacggacacc tacggtgtta agcaccagcg aaaagaacgt ccctgtgaag aaaaagaaag
     1321 ttaccgctgg cagcaggctt cggagcgcat ggccgaacgg ctgagggaga tacagaaaca
     1381 ggtaatcact gtctgtgacc gggaagcgga tatctggcat tacctgcatt ataaagtcag
     1441 ccgcgggaag ccacacgctg aatgtgatgc aaaaaggcgg gcgggcagcc cgccaggccc
     1501 ggatgctcat cagttacagc gaagtcagca taaaaaatcc cgacaacagc ggccaggcgc
     1561 tctcgctcac gtatgtctgt tgcggggagc aggagaggac ggagcctgct ggtatccgct
     1621 gacatcagaa aaagtggcga gtgccgccga tgcgcgacgt atcgtcagcc attacgagca
     1681 acgctggctg atagaggaat accacaaagc gtggaaaagt ggtggtacat gtgtggaatc
     1741 gctgcgaatg cagacccggg ataaccttga gcacatggtg gttatcgcgg tgcgggtgct
     1801 gggcctgcgg cagggaggta tcagtgaaga aacacaaaat gacagctgtg agaaaatact
     1861 gacgccgaca gagtggaaac tactgtgggt taagctggaa ggaaagcagc ttccggcgca
     1921 aacaccgacc ctgaaatggg cctgtttgaa actggctaaa ctggggcgat ggcatgacag
     1981 caaacgcaca agcagccccg gttgggtagt tatgtgggat ggctggttca gacatcagga
     2041 tatggctgag ggttacctgg tgatgaagtc tcttgatcag gagatctgat caagagacag
     2101 gtcaaagatg ggggacaacg tctggtgctg tataacagca caggcctcac actcttgacg
     2161 cccgtggaat atattagaga acaaaaattt caatatgtgg tggacgcata cagaggattg
     2221 acagcattcc catactaatt gttaagattc aaattgaagt atatctatct gtcattttgc
     2281 aaatgatatg cggtagcttt acctaactat catgaacatt tcgaaaaatc atccacaaag
     2341 ccccgaagaa taaagcacta tcgcccgata aagacctgta agctcagtgg catggatact
     2401 attgagctca gaatggcgag ctgtaccgtt atttcattac catgctcgac gaacacagca
     2461 actactctct ggccgtacta tcgcttaaaa gtgatatcat caggcatttc ttctgctgtg
     2521 tagcccagtt gttccttgtt ggtatcaacc agatcatcac tgatgatggg caaagagttc
     2581 tcgggaaaat tcgacaaaac gctacaggaa gccaccgtcg ggttcgtatg gacttatctc
     2641 tatacgccga aaataaatgc cataagtgag cattttaacc gaacactgag ggaacaattt
     2701 attgagttta atgaagaatt gctctttgaa tagctgaata tgttcaatct gaaactggct
     2761 ga
//
LOCUS       DBFIFI010000340         2717 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.342, whole genome shotgun sequence.
ACCESSION   DBFIFI010000340 DBFIFI010000000
VERSION     DBFIFI010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.342"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..253)
                     /locus_tag="N7B39_004784"
     CDS             complement(<1..253)
                     /locus_tag="N7B39_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HGU2424594.1"
                     /translation="MKWLLLITLSLYSFIVQSAPCGLTNVGEQRGTYILKPLSMKGNL
                     TAEKIENTGNIIECADLQETTNAIKVHFLTQNALRFKVGS"
     gene            complement(266..2716)
                     /locus_tag="N7B39_004785"
                     /pseudo
     CDS             complement(266..2716)
                     /locus_tag="N7B39_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
BASE COUNT          765 a          561 c          545 g          846 t
ORIGIN      
        1 gacttccgac tttaaacctt aaagcgttct gtgttaaaaa atgaacttta atggcgttgg
       61 tcgtctcttg caagtctgca cactcaatga tattcccagt attttcaatt ttttcggcgg
      121 tgagattacc tttcatactc agtggtttga gtatatatgt tccacgttgt tccccgacat
      181 tggtcaatcc acagggagca ctttgcacaa taaaagaata tagactcaat gtgattaata
      241 aaagccactt catagtaaac tccttttact ggcatatata tgttttgttt tcatcgataa
      301 cgtgttgaaa agtaatagta cagaataaat tctgttcatc attgagagca acttttatgc
      361 cagttggaat ttcatctacg cgaataaaaa ggcgactacc ctggccaacg actccaatat
      421 tgttttcctg gagatccagt acgtcatagc caaaagttaa tggcgaacca tcgggacgca
      481 gtgcctgtat ataccagggc ttacgttggt cagttgtaaa ctgcacataa gaaactgctc
      541 cacggtaagg cgcaataatt tgacgattgc cctgcaattc tgtttcactt tcactgtttg
      601 ctgtatcaag taccagatgg ttttcacgat gaggtatcat caggtcgtaa acaacctggc
      661 ctttgctgtt tgttcggttg tatttttgtc cattaatatg cgcgccttct aacccaggcg
      721 catccagaat tgcaaatgta tcggatagct ggttagtgat attaatacca ccgggccaga
      781 caactaatcc actactaata cttccaccac tttgctaggc gtttttagaa tggctatagc
      841 tgccatctat tgtggcgatg ggagttctcc agcttaaatt cgtaccaagc gatgtatcat
      901 tattttgttg ttgctgatta acatatatac cataatttaa ctgatcatgt tcaccggcta
      961 tgccagtaat tccagtattg ttggaggaat agccatcttt ggaaaatgag gtcgaattcg
     1021 ataagttaat ttggtgacgt gttttggcaa tatcatcccc ccagtagaaa ggaatggaga
     1081 taaacagatt aaaacgctcc tcttctttgt cgttttcatc ataggattgg ctggcagaga
     1141 aagtataact gatgcgttgc cagctattgg aataactgaa ttggtaatct ttagcatttc
     1201 cacttcgccc ccagtaattc cgccaaagcg cacttaatga tacatttccc agattattgg
     1261 ataaaggttg catgatattg gcagaaaggc tattttttct gccaatatca taaaaatcgt
     1321 catagtcact ctgatgattt attttatcat tttcgtaaag atggtcgctg aatgtcctgt
     1381 aatcctgcga agcataacgc caggcggcca cgctaaatcg agtggccgtt tgtactagat
     1441 atttattata ggcgacctga tagctcgtac cttcgtgtgt tatatcatta tttaacttac
     1501 tactcgatcg tgtagcgtca aaagagatgg cacccagcgg tgtattccaa ccatttccca
     1561 gagttatagc attatagtta tcggataaaa tcgtaccgcc ataaagtgtc aaaagattgt
     1621 tgaggccata tatataattt gcctcgagaa agtcctcctg attctttaca ccatatatct
     1681 tactgcgtcc ggcgataaaa tcgaaatttg aaataccagg ttgcagcatg ttaggcacgg
     1741 aagagtaggg caccaaaaag gaacgaacac tgccatctgc ttcctttatg ctgacatcga
     1801 gatcagagcc gctgcccgag agttgcaaat ctgcgatagt aaatggtccc gggggaacct
     1861 ctttttggta aatgatgtag ccattttgcg aaaccgttat caaggcatta ctttgcgcaa
     1921 caccttgtac taaaggtgta aagctctgca tagaatcagg tagcatttgc atatcgcgaa
     1981 ataagcgtat tccgctaaat ctcagagagt cgaatatgag tgaggatgtg taattttctc
     2041 ctatctgaac tgtgcttaat atctgcgacc agccatgttc caggtaaaga gtattacttt
     2101 gccatgtgcc tttcatatca tcgggtttgc tatagctggc atcagagtgt aactgccaac
     2161 taaataaatt taatccactg ttaaatcggc cataactggc tgtattactg ttattattgc
     2221 tgtcataaga gcgatactga cttaaattat aggaggtata aaaagcatca atgccgcgat
     2281 cccaactttc cgggggaacg taacctcttt caagttcatt gatatacgcc tgtggtactg
     2341 ttaaatttag tcggtgttca ctgatatccc actgatattg tccaccatga accgctttat
     2401 ccagtaaaat acaattatct tctgtattga ggttaccggt ctttaccccc agtttggtta
     2461 gtagcacctt aggcagacat ggtttgactg gtgactctgg gatcgtgata tcttgttttc
     2521 cacgccactg gttatttaca taaaaatcaa gttcgtagtt tcctggaagc ggttgtttat
     2581 tatcgaaatg atattcggaa actttttctc ctttcatgcc gccgatcata aagtgcgtat
     2641 caaaagtttc ttcagcataa cccggaaaag aatacaaaaa aataatagca gcgacccatc
     2701 gtgtcgtcct taacatg
//
LOCUS       DBFIFI010000341         2663 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.343, whole genome shotgun sequence.
ACCESSION   DBFIFI010000341 DBFIFI010000000
VERSION     DBFIFI010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.343"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            54..2333
                     /gene="ydeP"
                     /locus_tag="N7B39_004786"
     CDS             54..2333
                     /gene="ydeP"
                     /locus_tag="N7B39_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HGU2424595.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAQNTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSVEFLARLGERYGFTPPHVPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQATLPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2581..>2663
                     /gene="safA"
                     /locus_tag="N7B39_004787"
     CDS             2581..>2663
                     /gene="safA"
                     /locus_tag="N7B39_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HGU2424596.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVIL"
BASE COUNT          713 a          622 c          654 g          674 t
ORIGIN      
        1 tgcatcatcg ctattacaaa tcctaataat tcatttccac acaggataag tagatgaaga
       61 aaaaaattga atcctaccag ggtgctgccg gtggttgggg tgctgttaaa tccgtagcga
      121 atgcagtacg taagcagatg gatatacgcc aggatgttat tgccatgttt gacatgaata
      181 agccagaggg ctttgactgt ccgggttgtg catggccaga tcctaagcac agtgcgtcat
      241 tcgacatttg tgaaaacggc gcaaaagcaa tcgcctggga agtcacggat aagcaggtaa
      301 atgcctcttt ctttgctcag aatacggttc aatcattact tacctgggga gatcacgagc
      361 ttgaggctgc gggacgtctc actcagcctt tgaaatatga tgctgtcagc gactgttaca
      421 agccattaag ctggcaacaa gctttcgacg aaattggcgc acgccttcaa agctatagtg
      481 atcccaatca ggttgaattc tatacttcgg gccgcacttc caatgaagct gcctttcttt
      541 atcagctttt tgcccgtgaa tacgggagca ataactttcc cgactgctcc aacatgtgcc
      601 atgaaccgac aagcgtgggt ttggcagcga gtatcggtgt aggtaaaggg accgtgttgc
      661 tggaagactt tgagaagtgc gatttagtca tttgcattgg gcataaccct ggtacaaacc
      721 accctcgcat gctgacttcg ttgcgcgctt tagtgaaacg gggagcgaaa atgatcgcca
      781 tcaatcctct acaggaacgt ggcctggagc gatttaccgc accgcaaaac ccgtttgaaa
      841 tgctgacgaa ctctgagact cagttggcca gtgcctacta taacgtgcgc attggtggtg
      901 atatggcgtt gctcaagggg atgatgcgcc tgttaattga gcgcgatgat gctgcaagcg
      961 ccgcaggtcg gccctcattg ctggatgacg aatttattca aacgcatacc gtcggctttg
     1021 acgagctacg ccgtgacgtg ctcaattccg agtggaaaga tatcgaacgt atttctggac
     1081 taagtcagac acaaatcgcc gaactggctg atgcatatgc cgctgccgaa cgaaccatta
     1141 tctgttacgg aatggggatc actcagcacg aacatggtac ccagaatgta cagcaactgg
     1201 tcaatctgct gttgatgaaa ggtaacattg gcaagcctgg tgcgggtatc tgcccactac
     1261 gtggccactc taatgtacag ggcgaccgaa ccgtcggtat caccgagaaa ccgtctgtag
     1321 agtttctggc tcgcctgggt gagcgctatg gcttcacccc acctcatgta cctggacatg
     1381 ctgcaattgc cagcatgcaa gcaatatgta cggggcaggc tcgagcattg atctgcatgg
     1441 ggggcaactt tgcactggca atgccagatc gggaagcgag cgctgtaccg ttaacgcaat
     1501 tagatttggc ggtacacgta gccactaagc ttaaccgctc tcatctgctg accgcacggc
     1561 atagctatat tctgccggtt ctgggacgta gcgagattga catgcaaaaa agcggtgcgc
     1621 aggcggtaac cgttgaggat tcaatgtcga tgattcatgc ctcgcgtggc gtgttaaaac
     1681 ccgccggtgt aatgctgaaa tcagagtgtg ctgtggtcgc gggaatcgcg caggcaacac
     1741 taccccagag cgtggtagcc tgggagtatc tggtggaaga ttatgatcgc attcgcaatg
     1801 acattgaagc tgtgctgcca gagttcgccg actataacca acgcatccgt catcccggtg
     1861 gttttcacct gataaatgca gctgctgaaa ggcgctggat gacgccgtca ggtaaggcta
     1921 atttcattac cagcaaaggg ctgttagaag atccctcttc agcgtttaac agtaagctgg
     1981 tcatggcgac agtacgcagc cacgatcagt acaacacgac gatttatggt atggatgatc
     2041 gctatcgagg ggtattcggt caacgagatg tggtctttat gagtgctaaa caagctaaaa
     2101 tttgccgtgt aaaaaacggc gaaagagtta atcttattgc gcttacgcca gacggtaagc
     2161 gcagctcacg ccgcatggat agattaaaag tggtcattta ccctatggct gaccgctcac
     2221 tggtgaccta ttttccagaa tcgaatcaca tgctaacact tgataaccac gatccattaa
     2281 gtggcattcc tggctataaa agtattccgg ttgaattaga accatcaaat taatgcctct
     2341 tctcatttct tctgctgtca tccgcacagc agaagaattc ctcattgatt attatcgcgc
     2401 aatttgctca catggattaa attaaactac atactataag atataaactt ctgcctacag
     2461 ctgtaagaaa ctccgctcag tactgaagca ccagtcctat ttcctctttt ctcaagcctg
     2521 ttatattaag catactgatt aacgattttt aacgttatcc gctaaataaa catatttgaa
     2581 atgcatgcga ccacagtgaa aaacaaaatc acgcaaagag acaactataa agaaatcatg
     2641 tctgtaattg tggttatctt att
//
LOCUS       DBFIFI010000342         2649 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.344, whole genome shotgun sequence.
ACCESSION   DBFIFI010000342 DBFIFI010000000
VERSION     DBFIFI010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.344"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..469
                     /locus_tag="N7B39_004788"
     CDS             <1..469
                     /locus_tag="N7B39_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HGU2424597.1"
                     /translation="DKAMVNYAFDYLSSPGSLPFTTAATELSAIHGHSTSQYRLGEFY
                     LHGSDGKPLDYTQARYWYEQSAEQENPRAQSKLGWIYLKGLGVKPDTRKAILWYKEAA
                     EQGYAHAQYTLGLIYRNGSGINVNHYESQKWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(479..1906)
                     /locus_tag="N7B39_004789"
     CDS             complement(479..1906)
                     /locus_tag="N7B39_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HGU2424598.1"
                     /translation="MKNCWKILNIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMNEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQIILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWHEYPSTQADALMLQWCRQHRVDYYPLVVMMIEARELVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGCKPRISLTRDTEHTLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAENRCPLEALIIRRLLIQAANFTEKQTVEPEPQPQPMPVDDGGPGC
                     LGIIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(1903..2610)
                     /locus_tag="N7B39_004790"
     CDS             complement(1903..2610)
                     /locus_tag="N7B39_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HGU2424599.1"
                     /translation="MDMESQKILFALSTPMEIRNECCLPSHSSPKMYLGTRFFDLSSS
                     WGIDDRDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDNLPLLREFLARKEYDDSGNDMFYQLFASDDAYYATLPWQP
                     LADYPTCPETLKDMSDL"
BASE COUNT          747 a          630 c          590 g          682 t
ORIGIN      
        1 tgataaagcc atggtgaatt atgcctttga ttatttaagc tcaccgggca gtcttccatt
       61 caccacggca gccacggagc tttccgcgat tcatggtcac tcaacgtcgc aatatcgcct
      121 tggagaattt tatcttcatg gtagcgacgg taaaccactg gattatacac aggcgagata
      181 ctggtatgag caatcagcgg aacaggaaaa tccacgcgcg caaagtaaac tggggtggat
      241 ctacctcaaa ggtctggggg tcaaacccga cacccgtaaa gcaattctct ggtataagga
      301 agcagctgaa caagggtatg ctcatgctca atatacttta ggtttgatct acagaaatgg
      361 ctcaggtatt aatgttaacc attatgaatc tcaaaaatgg ttaaaactgg ccgccaaaca
      421 acattacaaa aatgcggaaa gattacttgc cgggcttccc gcacattaac gtcgactgtc
      481 acccgaacag atggagtatt ttccctatca aaccagcaaa gataaaaata tagaaaataa
      541 ttttaatgat gcccagacag cctggcccac catcgtcaac gggcattggc tgcggttgcg
      601 gctccggttc aacagtttgc ttctcggtaa aattcgccgc ctgaattaat aatcgacgaa
      661 taattaatgc ttccagtgga caacgatttt ctgcatcaag acggcttacg atcggcatta
      721 atgattcttc tgtaggttgg ctggcctgca ctaattgttt ggcaactaga tataatggcc
      781 ataaggtatg ttctgtatca cgcgtgagtg agatccgcgg cttacatcct ttgtgcaaaa
      841 ccatctcgac cagtgaacgc cccagagcag agagtttttc atcgctgagt atttcgtata
      901 aatgaaaacg cgtacgggcg ctgtcgccgg ggacataaag tagcggtttt ccctggtcgt
      961 taaccagttc acgagcttca atcatcatca ccactaatgg gtaataatcg actcgatgct
     1021 gacggcacca ttgcaacatt aaagcatccg cctgagtgga gggatactcg tgccagtagt
     1081 cacaaagttc tgccagcagg ctttcgcctt ctccacaata gacgcgttgc gcgtattcat
     1141 aataacgcgc atcttcatta tcaggttcag cttcccgcca cgaccgggcg taatccagga
     1201 gttcggggat tcccaggcga cctgcactat accagcggag taaatttctg tgcagttttg
     1261 catcatcggg gataatcact gcgcgatgtt gcgctaaata caatgtcagc cattctggat
     1321 gcgatgacca aatccgggcg aacatttggt aaaaatcgat ggtgtcattt tgcacggcaa
     1381 cgggcagatg cagaatgatc tggaagttaa aaacatttcc cttcgcaatg gcatataaaa
     1441 aggattccag ttcttcttcg tctatttccc cgacgttttc ctcctgcaac caccgcaatc
     1501 tttctgctaa taacaccacg cactcgaagg ataaatgggc agtgttcatg gctattgtgc
     1561 acagcgacca gcgtaattca tcaatctcat tcatcgagca ataatttaat tgctgaatga
     1621 atcgctgcca ggcggagggc agaaaacgtt cactatcaga ggcaagtaac gcacgaaagg
     1681 cgagcagaat ttcatgttct gctacctcat attcttctgg ttgccaaaca gatttagcag
     1741 gcgactgcgc aatccggagc gcttcctcat acgcctgacg aagttgttta aaaccctgcg
     1801 gatcggtttc tggatgaaag gacggtaaca gcgccagata agcgcggcgg ataatatcga
     1861 catcagtcgt ttcctctatg ttgaggatct tccagcaatt tttcataggt cgctcatatc
     1921 cttaagcgtt tccgggcatg tggggtagtc agccagtggt tgccagggta aggtcgcgta
     1981 atatgcatcg tcactggcaa ataattgata aaacatatca ttgccggaat cgtcgtattc
     2041 tttacgcgct aagaattcac gtaataaagg cagattatca tcttcgggag actgccaata
     2101 ttgccgacca aaggtataac cggcaaagta ttgccgccag ttactataat aacgtagagc
     2161 ccgtagatga atacgcgact ggatccatag actttcttct tcactcaacc agttattaag
     2221 tacgcccatt cgacttaaaa aacccatccg gacataatcc catgctttaa ttcctccctc
     2281 accacagcat tcggctgtgc tggcgacaaa ttgcgcataa gcctgacctt gctcatttaa
     2341 ttcagcaaga tagtcacgcc attcacatgg agaataacga aaccagcggt gataaaaccc
     2401 tgccaaccgc gcggcatgac cgttatcgat cattcggtga attgtgcgca gcaggtcatc
     2461 acggtcatca attccccagg aggaagaaag gtcaaaaaag cgtgtaccta aatacatttt
     2521 gggtgatgaa tgcgaaggta aacagcattc attgcgtatt tccatcggag tggaaagtgc
     2581 aaacaatatt ttttgcgact ccatgtccat atgatttatc cttaaatatt cattgaatga
     2641 atattagcc
//
LOCUS       DBFIFI010000343         2618 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.345, whole genome shotgun sequence.
ACCESSION   DBFIFI010000343 DBFIFI010000000
VERSION     DBFIFI010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.345"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..353)
                     /locus_tag="N7B39_004791"
     CDS             complement(<1..353)
                     /locus_tag="N7B39_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000708831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424600.1"
                     /translation="MKKAAILIDAGFFMQRVHATHRKHFAEHELTAQCIMKVIWSMVL
                     SHLNGKRQSQERREPLELYRIYFYDCPPLDIQTRLPLPEPGNKTPGRKNFKLEKSYIL
                     RTELHEELRKTRKTA"
     gene            complement(481..1194)
                     /locus_tag="N7B39_004792"
     CDS             complement(481..1194)
                     /locus_tag="N7B39_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HGU2424601.1"
                     /translation="MSAKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMG
                     QSGVGALFNGINALNAYNAALLAKILNVSVEEFSPSIAREIYEMYEAVSMQPSLRSEY
                     EYPVFSHVQAGMFSPELRTFTKGDAEKWVSTTKKASGSAFWLEVEGNSMTAPTGSKPS
                     FPDGMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNE
                     SCSVVGKVIASQWPEETFG"
     gene            1295..1495
                     /locus_tag="N7B39_004793"
     CDS             1295..1495
                     /locus_tag="N7B39_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HGU2424602.1"
                     /translation="MEQRITLKDYAMRFGQTKTAKDLGVYQSAINKAIHAGRKIFLTI
                     NADGSVYAEEVKPFPSNKKTTA"
     gene            1614..1907
                     /locus_tag="N7B39_004794"
     CDS             1614..1907
                     /locus_tag="N7B39_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda phage CII family protein"
                     /protein_id="HGU2424603.1"
                     /translation="MVRANKRNEALRIESALLNKIAMLGTEKTAEAVGVDKSQISRWK
                     RDWIPKFSMLLAVLEWGVVDDDMARLARQVAAILTNKKRPAATERSEQIQMEF"
     gene            1940..2618
                     /locus_tag="N7B39_004795"
                     /pseudo
     CDS             1940..2618
                     /locus_tag="N7B39_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
BASE COUNT          770 a          585 c          588 g          675 t
ORIGIN      
        1 atggctgttt ttcgagtttt tcttaactct tcatgcagct ccgttctcag aatatatgat
       61 ttttcgagtt tgaaattctt gcgaccaggc gtcttattgc caggctcagg aagtggaagg
      121 cgtgtttgaa tgtcgagtgg tggacagtca tagaagtaaa ttctataaag ctcaagcggt
      181 tccctacgtt cttgtgattg acgttttcca ttaagatggg aaagaaccat tgaccatatt
      241 actttcatta tgcattgcgc agtcagttca tgctcggcga agtgtttacg atgcgtagca
      301 tgaacacgct gcatgaaaaa acccgcatca attaaaattg ctgctttctt cataagagtc
      361 ccaaaaaaat agcccagagc cgttatgcag atattaacaa ttgtctgcga acggggctgg
      421 gcttggttta attaatctat gccaagttga ctgtgccgtc aacataaaac ttacttccta
      481 tcacccaaac gtctcttcag gccactgact agcgataact ttccccacaa cggaacaact
      541 ctcattgcat gggatcattg ggtattgtgg gtttagtggc tgtagaaaca cctgaccgct
      601 atccctgatc agtttcttga aggtaaactc atcaccacca agtctggcta tacagaaatc
      661 gcctggctca acagcctgct cagggtcaac cagaattaac atcccgtcag gaaagctggg
      721 cttggatcct gttggtgcgg tcatggaatt accttcaacc tcaagccaga atgcagagcc
      781 actggctttt ttggttgtgc ttacccattt ctccgcatcg cctttggtaa aggttctaag
      841 ctcaggcgag aacatcccgg cctgaacatg agaaaaaaca gggtactcat actcacttct
      901 aagtgacggc tgcatactaa ccgcttcata catctcgtag atttctctgg cgattgaagg
      961 gctaaattct tcaacgctaa cgttgagaat ttttgcaagc aatgcggcgt tataagcatt
     1021 taatgcattg atgccattaa ataaagcacc aacgcctgac tgccccatcc ccatcttgtc
     1081 tgcgacagat tcctgggata agccaagttc atttttcttt ttttcataaa tagctttaag
     1141 gcgacgtgcg tcctcaagct gctcttgtgt taatggtttc ttttttgcgc tcatacgtta
     1201 aatctatcac cgcaagggat aaatatctaa caccgcgcgt gttgactgct ttacctctgg
     1261 cggtgataat ggttgcatgt actaaggagg ttgtatggaa caacgcataa ccctgaaaga
     1321 ttatgcaatg cgctttgggc aaaccaagac agctaaagat ctcggcgtat atcaaagcgc
     1381 gatcaacaag gccattcatg caggccgaaa gattttttta actataaacg ctgatggaag
     1441 cgtttatgcg gaagaggtaa agcccttccc gagtaacaaa aaaacaacag cataaataac
     1501 cccgctctta cacattccag ccctgaaaaa gggcatccaa ttaaaccaca cctatggtgt
     1561 atgcatttat ttgcatacat tcaatcaatt gttatctaag gaaatactta catatggttc
     1621 gtgcaaacaa acgcaacgag gctctacgaa tcgagagtgc gttgcttaac aaaatcgcaa
     1681 tgcttggaac tgagaagaca gcggaagctg tgggagttga taagtcgcag atcagcaggt
     1741 ggaagagaga ctggattcca aagttctcaa tgctgcttgc tgttcttgaa tggggggtcg
     1801 ttgacgacga catggctcga ttggcgcgac aagttgctgc gattctcacc aataaaaaac
     1861 gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga
     1921 tctatcaaca ggagtcatta tgacaaatac agcaaaaata ctcaacttcg gcagaggtaa
     1981 ctttgccgga caggagcgta atgtggcaga tctcgatgat ggttacgcca gactatcaaa
     2041 tatgctgctt gaggcttatt cgggcgcaga tctgaccaag cgacagttta aagtgctgct
     2101 tgccattctg cgtaaaacct atgggtggaa taaaccaatg gacagaatca ccgattctca
     2161 acttagcgag attacaaagt tacctgtcaa acggtgcaat gaagccaagt tagaactcgt
     2221 cagaatgaat attatcaagc agcaaggcgg catgtttgga ccaaataaaa acatctcaga
     2281 atggtgtatc cctcaaaacg agggaaaatc ccctaaaacg agggataaaa catccctcaa
     2341 attgggggat tgctatccct caaattgggg gattgctatc cctcaaaaca gggggacaca
     2401 aaagacacta ttacaaaaga aaaaagaaaa gattattcgt ccgagaattc tggcgaatcc
     2461 tctgaccagc cagaaaacga tctttctgtg gttaaaccgg atgctgcaat tcagagcggc
     2521 agcaagtggg gaacagcaga agacctgacc gccgcagagt ggatgtttga catggtgaag
     2581 accatcgcgc catcagccag aaaaccgaat tttgctgg
//
LOCUS       DBFIFI010000344         2610 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.346, whole genome shotgun sequence.
ACCESSION   DBFIFI010000344 DBFIFI010000000
VERSION     DBFIFI010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.346"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..1757)
                     /locus_tag="N7B39_004796"
     CDS             complement(<1..1757)
                     /locus_tag="N7B39_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU2424604.1"
                     /translation="MWNLLRRTRKNQKSGRDVREAGWTSLFQAVAEPFSGAWQQGVKA
                     DPEAVLSFHAVFACISLISQDIAKMRLRLMQTDAHGIRRETRRGDIARLCRRPNAQQN
                     RIQFFELWLNAKLRHGNTVVLKIRNARGQIKELRILDWSRVEPLVADDGEVFYRITPD
                     RNCGITEAVTVPAREVIHDRFNCFFHPLIGLPPVYAAGLAATQGHHIQENSTSFFRNG
                     GRPSGVIEIPGSITEENAKKLKSNWDSGYTGENAGKTAILSNGAKYNPTTFSPVDAQT
                     VEQLKMTAEIVCSVFRVPAYKIGVGQPPSSDNVEALEQQYYSQCLQTLIESIELLLDE
                     ALETGENESTEFDVTTLLRMDSERRMKTLGDAVKNTLLTPNEARKRENLPPLAGGDAL
                     YLQQQNYSLEALSRRDAREDPFASAGKTVSSQLPDGASDGNKAISETEHDAVKAMFRG
                     DTEKMTERELSIIRALGEEFSTVLADLQRTFEGKMASQAQAFEEKLTSLSAVLQKHVT
                     VDEVRPVLQAMVDDAVGAIPVPRDGRDYDPDVLQQAVNDAVANIPVPADGKSITPDDV
                     RPMLEQMVKEAVSHIPVPR"
     gene            complement(1703..1924)
                     /locus_tag="N7B39_004797"
     CDS             complement(1703..1924)
                     /locus_tag="N7B39_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424605.1"
                     /translation="MAKIRYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITVRRPA
                     GQKKKRDAEHGECGTFYGEPEKTRNQDVT"
     gene            complement(1969..>2610)
                     /locus_tag="N7B39_004798"
     CDS             complement(1969..>2610)
                     /locus_tag="N7B39_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HGU2424606.1"
                     /translation="LIRFSSPAADALVRNALAEAVVARLDTDFVDPKKAAVADVSPAS
                     ITHDVKGTASTGNPDADAEAAFGQFVAANLQPTGAVWLMSSTNALALSMRKNALGQKE
                     YPDMTLLGGSFQGLPVIVSQYVGDQLVLVNAPDIYLADDGGVAVDMSREASLEMQSEP
                     GGDSTTPSPVELVSMFQTGSVAIRAERWINWRRRRTAAVAVITGVNYGSASGG"
BASE COUNT          525 a          858 c          651 g          576 t
ORIGIN      
        1 tcgcgcggaa caggaatatg gctcactgcc tctttcacca tctgctcaag catcggacgc
       61 acatcatccg gggtgatact tttgccgtct gccggtaccg gaatatttgc gaccgcatca
      121 ttcaccgcct gctgcagtac atccggatca taatcacgac catcacgcgg taccggaatg
      181 gcccccacag cgtcatccac catcgcctgc agaaccggac gcacctcatc caccgtcaca
      241 tgcttctgta ataccgccga cagggaagtc agtttctctt caaacgcttg tgcctgcgag
      301 gccatcttcc cctcaaatgt gcgctgtaaa tccgccagca ctgtggaaaa ttcttctccc
      361 agtgcacgaa taatggacag ttcccgttcc gtcattttct cagtatcccc cctgaacatc
      421 gctttcaccg catcatgctc tgtttcactg attgccttat taccgtcaga tgcgccgtca
      481 ggcagctgtg atgaaactgt tttcccggca gacgcgaacg gatcctcacg ggcatcacga
      541 cgggacagcg cctccagact gtagttctgc tgctgaagat acagtgcatc accgccggcc
      601 aggggcggca ggttctcccg tttacgggcc tcattgggcg tgagaagcgt atttttcacc
      661 gcatccccca gcgttttcat gcgccgctca ctgtccattc tcagcagcgt ggtgacatca
      721 aattctgtac tctcgttttc ccccgtttcc agcgcctcat ccagtaacag ttcaatggac
      781 tcaatcagcg tctgcaggca ctgggaataa tactgctgct ccagcgcctc cacgttgtca
      841 ctggaaggcg gttgtcccac gccaatcttg taggccggga cacggaacac cgaacagaca
      901 atttcagcgg tcatcttcag ttgttccacc gtctgcgcat ccaccggtga aaacgtcgtg
      961 gggttgtatt ttgccccgtt gctcagaatg gccgttttcc ccgcattttc gcctgtatac
     1021 ccgctgtccc agttgctctt cagttttttc gcattttctt ccgtaatact gccggggatc
     1081 tcaatcaccc cggacggcct gccgccattt ctgaaaaaag acgtcgaatt ttcctgaata
     1141 tgatgcccct gcgtggccgc cagcccggcg gcatacaccg gcggcaatcc tataagcgga
     1201 tgaaaaaaac agttaaaccg gtcgtggatc acttcccggg caggcaccgt caccgcctcc
     1261 gtgatcccgc agttccggtc cggcgtgatg cggtagaaca cctcgccgtc atccgccacc
     1321 agaggttcaa cccggctcca gtccagaata cgcagttctt tgatctgccc ccgggcatta
     1381 cggattttca gcaccaccgt attgccatga cgcagtttgg cgttcagcca cagttcaaaa
     1441 aactggatgc ggttctgctg ggcgttggga cgacgacaga ggcgggcaat atccccccgg
     1501 cgcgtttccc tgcgtatccc atgcgcatcc gtctgcataa gacgcagccg cattttggcg
     1561 atatcctggg atatcagcga aatacatgca aacaccgcat gaaaggagag gacggcttca
     1621 ggatcggctt tcacgccctg ctgccaggcg ccggaaaagg gctcagccac cgcctgaaac
     1681 aggctggtcc agcccgcctc tcttacgtca cgtcctgatt tctggttttt tcgggttcgc
     1741 cgtaaaaggt tccacattcg ccatgctccg catcacgttt ctttttctga cctgccggac
     1801 gtcgcaccgt gatgtactcc gcctttccca ggcgaaccag cacctccgca cacggctgtg
     1861 ccacatcacg gatatccccg gcccgggcat catgcgtgcc ctgcagatat cggatctttg
     1921 ccataacctg ttacgggagg cgcacgcctc ccgtcctcct tatcagactc agccgccgga
     1981 cgcactgccg tagttcactc cggtgatcac cgccaccgcc gcagtacggc gacgacgcca
     2041 gttgatccag cgctccgcac ggatggccac gctgcctgtc tggaacatgg aaaccagctc
     2101 caccggggac ggcgtggtac tgtcgccgcc cggctcagac tgcatttcca gtgatgcctc
     2161 gcgggacata tccactgcca cgccgccgtc atccgccaga taaatatccg gggcattcac
     2221 cagcaccagc tggtcaccca cgtactggga gacaatcacc ggcagcccct ggaaggagcc
     2281 acccagcagg gtcatgtccg ggtattcctt ctgacccagc gcatttttac gcatggacag
     2341 tgccagggca ttggtgctgg acatcagcca gaccgcaccg gtgggctgca ggtttgctgc
     2401 cacaaactgt ccaaacgcag cctcggcatc cgcatccggg ttaccggttg atgccgtgcc
     2461 cttcacatca tgggtgatgg acgccgggga gacatctgcc actgcggctt ttttcgggtc
     2521 cacaaagtct gtatccagac gcgccaccac cgcttccgcc agcgcattac ggaccagtgc
     2581 atcagcagcc ggactggaaa aacggatcaa
//
LOCUS       DBFIFI010000345         2563 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.347, whole genome shotgun sequence.
ACCESSION   DBFIFI010000345 DBFIFI010000000
VERSION     DBFIFI010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.347"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            411..554
                     /locus_tag="N7B39_004799"
     CDS             411..554
                     /locus_tag="N7B39_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HGU2424607.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            567..776
                     /gene="cspE"
                     /locus_tag="N7B39_004800"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             567..776
                     /gene="cspE"
                     /locus_tag="N7B39_004800"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HGU2424608.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            942..1751
                     /gene="rlmA"
                     /locus_tag="N7B39_004801"
     CDS             942..1751
                     /gene="rlmA"
                     /locus_tag="N7B39_004801"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HGU2424609.1"
                     /translation="MSFSCPLCHQPLTREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLNEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQKSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQILAAKEVLDCQTDFNIHLWQRSY"
     gene            complement(1748..2314)
                     /gene="mntP"
                     /locus_tag="N7B39_004802"
     CDS             complement(1748..2314)
                     /gene="mntP"
                     /locus_tag="N7B39_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HGU2424610.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT          679 a          604 c          616 g          664 t
ORIGIN      
        1 tcatcaattt ttcttatttt aaatttccaa ttcctttggg attgacttct ctttggggtg
       61 attgagagcc gttaactgcc tgttttatga gaaaaagtga tataactttt tattcattgc
      121 atagcagaaa atgtgatatt gcacgcacta tgtaataact tctcccactg acctggaaca
      181 actgaactta ttgaactatg ttagaaacta cgtcagttta agtatctgcg gaactgacaa
      241 ggttaagcac aatgatatat cggcgcgtat tccgttgcat aagtgtgcaa aaaaagtgga
      301 agacgtatcg agatttgtgc gtctgatcga gacatgttta aaaatggctt gccataatta
      361 acgttgtatg tgataacaga tttcgggtta aacgaggtac agttctgttt atgtgtggca
      421 ttttcagtaa agaagtcctg agtaaacacg ttgacgttga ataccgcttc tctgccgagc
      481 cttatattgg tgcctcatgc agtaatgtgt cagttttatc tatgttatgc ctgcgggcga
      541 agaaaacaat ctaaggaatt tttcaaatgg caaagattaa aggtcaggtt aagtggttca
      601 acgagtctaa aggttttggc ttcattactc ctgctgatgg cagcaaagac gtgttcgtac
      661 acttctccgc tatccagggt aatggcttca aaactctggc tgaaggtcag aacgttgagt
      721 tcgaaattca ggacggccag aaaggtccgg cagctgttaa cgtaacagct atctgatcga
      781 atccactgat ctgaagtgtg aatacgcttc aatctcgcta taaagcctcg tcgaatgcga
      841 ggctttttac tatgctttat cttcgctcct ggcgttcgga tatttgcccg ccgcgtgatt
      901 cgcgttacac ttgcggcctt tagtatcctg ccggagttgt catgtcgttt tcctgtccac
      961 tttgccatca gcctcttacg cgtgaaaaaa acagctatat ctgtccccaa cgacatcagt
     1021 ttgatatggc gaaagaaggg tatgtcaatc tgctgcccgt tcagcataaa cggtctcgtg
     1081 atccgggcga cagcgcggaa atgatgcaag cacgccgcgc attcttagat gccggacatt
     1141 atcagccgct gcgtgatgca attgtcgccc aactgcggga acggcttaat gaaaaggcca
     1201 cggcggtgct ggatattggc tgtggtgaag ggtattacac acacgcattt gccgatgcgt
     1261 tgcccgaaat caccacgttt ggtctggatg tttcgaaggt agcgataaaa gcggcggcga
     1321 aacgctatcc gcaggtcact ctttgtgtcg cttccagcca ccgtttgccg ttttccgata
     1381 ccagtatgga cgccataata cgtatttacg cgccgtgtaa agcagaagaa ttagcacgag
     1441 tagtgaagcc cggcggctgg gtcattactg ccacgccggg accgcgacat ttgatggagc
     1501 tgaaggggct gatttacaat gaagtacatc ttcatgcacc tcatgcagaa caactggaag
     1561 gttttacatt acagaagagt gatgagttgt gttatccgat gcgtcttcgc ggtgatgaag
     1621 ccgtcgcatt attgcagatg acgccgtttg cctggcgtgc gaagccagaa gtctggcaaa
     1681 tactggcagc aaaagaagtg ttagactgcc agacggactt taatattcac ctctggcagc
     1741 gttcttatta accgtggaag tgcgtccaga ggatctggac gccgatgccg atcagcacca
     1801 gcccgccgag aatttccgct tttttcccaa taattgagcc gataaagcga ccaaccatca
     1861 tccctaatgt cgacataatc aaggttgcac aaccaatggc caatgcggtc gcgataatgt
     1921 tgacctgaag gaaagcaaga ccaacaccca cagccatggc atccaggctg gtggcaatcg
     1981 cggtggttac cagtagccag aaaccgtgcc gacggcgcgg ctcttcatct tcatcatctg
     2041 cgccacgaaa accctcaata atcattcgcc cgccgaggaa tatcagcagc acaaacgcaa
     2101 tccagtgatt ccattcaagg acaaaccggc tggctaacat gcccattccc cagccgatca
     2161 gcggcgtcag ggtttcgacg gcaccaaaaa taaggccggt tcgcaatgct tcagaaaatt
     2221 tcggtttatg gagggtggca cctttaccga ttgatgcagc aaatgcatcc atcgacatac
     2281 caaacgcaag aagaacagta gcagtgatat tcatgacaac gtcctgaccg gggtatccat
     2341 aaaacacatc atcgccccca gcaaacatgc atacatgaac aacaagccgc gatgggtttt
     2401 cgcgttcatg caggcatagc gattgatgtg catatggtct cgcctgactg aaagtcttca
     2461 gtccgtacgt gccacgtttt gcaacgagta tgttgacacg tacatttccg gaatctggaa
     2521 attggctact ccccaaatga gggcgcaacc ttaacatatt ttg
//
LOCUS       DBFIFI010000346         2560 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.348, whole genome shotgun sequence.
ACCESSION   DBFIFI010000346 DBFIFI010000000
VERSION     DBFIFI010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.348"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..2122
                     /locus_tag="N7B39_004803"
     CDS             <1..2122
                     /locus_tag="N7B39_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HGU2424611.1"
                     /translation="YILSTLSLCISGIVSTATATSSETKISNEETLVVTTNRSASNLW
                     ESPATIQVIDQQTLQNSTNASIADNLQDIPGVEITDNSLAGRKQIRIRGEASSRVLIL
                     IDGQEVTYQRAGDNYGVGLLIDESALERVEVVKGPYSVLYGSQAIGGIVNFITKKGGD
                     KLASGVVKAVYNSATAGWEESIAVQGSIGGFDYRINGSYSDQGNRDTPDGRLPNTNYR
                     NNSQGVWLGYNSGNHRFGLSLDRYRLATQTYYEDPDGSYEAFSVKIPKLEREKVGVFY
                     DTDVDGDYLKKIHFDAYEQTIQRQFANEVKTTQPVPSPMIQALTVHNKTDTHDKQYTQ
                     AVTLQSHFSLPANNELVTGAQYKQDRVSQRSGGMTSSKSLTGFINKETRTRSYYESEQ
                     STVSLFAQNDWQFADHWTWTMGVRQYWLSSKLTRGDGVSYTAGIISDTSLARESASDH
                     EMVTSTSLRYSGFDNLELRAAFAQGYVFPTLSQLFMQTSAGGSVTYGNPDLKAEHSNN
                     FELGARYNGNQWLIDSAVYYSEAKDYIASLICDGSIVCNGNTNSSRSSYYYYDNIDRA
                     KTWGLEISAEYNGWVFSPYISGNLIRRQYETSTLKTTNTGEPAINGRIGLKHTLVMGQ
                     ANIISDVFIRAASSAKDDSNGTETNVPGWATLNFAVNTEFGNEDQYRINLALNNLTDK
                     RYRTAHETIPAAGFNAAIGFVWNF"
     gene            2431..2560
                     /locus_tag="N7B39_004804"
                     /pseudo
     CDS             2431..2560
                     /locus_tag="N7B39_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544635.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="vimentin yjdA"
BASE COUNT          742 a          571 c          581 g          666 t
ORIGIN      
        1 ttatatcctt tccactctgt cattatgcat ttctgggata gtttctactg caaccgcaac
       61 ttcttcagaa acaaaaatca gcaacgaaga gacgctcgtc gtgaccacga atcgttcggc
      121 aagcaacctt tgggaaagcc cggcgactat acaggttatt gaccaacaaa cattgcagaa
      181 ctccaccaat gcctccatag ccgataattt gcaggacatc cccggagtag agataacaga
      241 caactccttg gcaggccgta aacaaatccg cattcgtggc gaagcatcct cccgtgtttt
      301 aattctcatt gatggtcagg aggtaactta tcagcgcgcc ggagataatt atggtgtggg
      361 actgttgata gatgagtctg cgctggagcg tgttgaggta gtgaaaggtc catattccgt
      421 actgtacggt tcacaggcaa ttggcggtat tgttaacttc atcaccaaaa agggaggtga
      481 caaacttgca tctggagttg tgaaagctgt ttataattcc gcaacagcag gctgggaaga
      541 atcaatcgcg gtccagggga gcatcggtgg atttgattat cgcatcaacg gtagttattc
      601 tgatcagggc aatcgtgata cgccggatgg acgtctgccg aataccaact atcgtaacaa
      661 tagtcagggt gtatggttgg gttataactc cggaaaccat cgttttggcc tctcgcttga
      721 tcgctacaga ctcgcgacac aaacttacta tgaggatcca gacggaagtt atgaggcatt
      781 tagtgtcaaa atacctaaac ttgaacgaga gaaagttggg gtattctatg acacagacgt
      841 ggacggtgac tatctaaaaa aaattcattt cgacgcgtat gagcagacca tccagcgcca
      901 atttgccaac gaagtaaaaa cgacacagcc tgttcccagt ccgatgattc aggctctgac
      961 cgttcataac aagactgaca cccatgataa gcaatacact caggcggtca cattgcagag
     1021 tcacttttcg ctgcctgcta ataatgaact tgttaccggt gcacagtaca aacaagacag
     1081 ggtcagccaa aggtccggtg gcatgacctc aagcaaatct ctgaccggct tcattaataa
     1141 ggaaacacga actcgctcct attatgagtc agagcaaagt acagtctcac tattcgcaca
     1201 aaatgactgg caattcgccg atcactggac atggacaatg ggagttcgcc aatactggct
     1261 ttcttcaaag ttgacgcgtg gtgacggagt atcatatacc gcaggcatta taagcgatac
     1321 ctctcttgcc agagagtctg cgagtgatca cgaaatggta acatctacaa gcctgcgcta
     1381 ttcaggtttc gataacctgg agttacgcgc tgcgttcgcg caaggctacg tatttcccac
     1441 actctcccag ctttttatgc agacatctgc gggcggcagt gtcacatacg gaaatcctga
     1501 tcttaaggct gaacactcca ataactttga attaggtgca cgatataatg gtaatcagtg
     1561 gctgattgac agcgcagttt actactcaga agctaaagat tatattgcaa gtctgatctg
     1621 tgatggcagt atagtttgca atggtaacac caactcctcc cgtagtagct actattatta
     1681 tgacaatatt gatcgggcaa aaacatgggg actggaaata agcgcggaat ataatggctg
     1741 ggttttctcg ccatatatca gtggcaattt aattcgtcgg caatatgaaa cttcaacatt
     1801 aaaaacaact aatacaggtg aaccagcgat aaacggacgt atagggctga aacatactct
     1861 tgtgatgggt caggccaaca taatctctga tgtttttatt cgtgctgcct ctagtgcaaa
     1921 agatgacagt aacggtaccg aaacaaatgt tccgggctgg gccactctca actttgcagt
     1981 aaatacagaa ttcggtaacg aggatcagta ccggattaac ctggcactca ataacctgac
     2041 agacaaacgc taccgtacag cacatgaaac tattcctgca gcaggtttta atgcagctat
     2101 aggttttgta tggaatttct gatgacaaaa acaatcgtta tggaattctt tgtcctgatc
     2161 tgcccgcttt gcgggcaggc taacgaacgt attgtgtttg ttgaaggttt ttcgacgttg
     2221 ttttccatct gtgggtgaat atttattctt cccgccagca tgactctata ctcattgcta
     2281 cgacaatgac ttataaagct ggttactgaa cctatacctt tgtaattcaa aagtgatatc
     2341 agttcacaac gcaatactat ttttagatta tttactctca cccagatact gaatcaatgg
     2401 ataatacctt ttgatttatc ccgacgcgga agaaggtctc agtccggatt atgggggaaa
     2461 atttctcaat ggtccggtat caacaaccag ggccgggagg attacaggaa acgagatttt
     2521 gccccggaca gccccgaaat aaaattcagt gatcgcaggg
//
LOCUS       DBFIFI010000347         2536 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.349, whole genome shotgun sequence.
ACCESSION   DBFIFI010000347 DBFIFI010000000
VERSION     DBFIFI010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.349"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..>2536
                     /locus_tag="N7B39_004805"
     CDS             <1..>2536
                     /locus_tag="N7B39_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU2424612.1"
                     /translation="GSAVGMAGAFAGAFASGHLISLADEWSSVNARLKQASQSSDEFS
                     SSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSAGDVLKVTEAISTGLKISGAS
                     TAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIVRALAAGMGVARKDLKAMADDGK
                     LTADKVVPALISQLGILRDEYAAMPETVSSSITKVENAFMAWVGGANEASGVTKTLSG
                     MLNGVAGQIDNVATAVGALVAVGVARYFGNMASGAMSATAGLVTAARNEVALAEAQFR
                     GTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAATQERLNRNIAARTAAQNALN
                     STTAVGSRLMSGALGLVGGVPGLVMLGAAAWYTLYQNQEQARESARQYALTIDEIAHK
                     TPSMSLPEASDNEGRTRAALTEQNRLIDEQASRVKSLQEKIAGYQYVLANPGWTTGDG
                     FMINHLTSVKTVTEGLAQATEQLAVEQSRLAQMQEKAQSIQDVLAGLEDRRVALIRQQ
                     AAEQNKVYQSMLVMNGQYTEFNRLLGLGNELLQQRQGLVNVPLRLPQATLDDKQQSAL
                     TKTERELALSRLKGEEKERVRLGYAADDLGFVGDPYQEARQRYISNALEAWRNNEVNK
                     PKSRGGKSETEKAEDSFSRLLKQQKEQLALVGQNTELAKLKYQTALGELKTLTEMQKQ
                     ELLRNATLIDQQKIREQLRSREETLKNENAAARASNDAELLGYGQGERARERMRELQQ
                     IRDSFRQKDADLQSQYQTGDISEDFYRQALAQNAQYLSERLKDQAVFYAESDVQRADW
                     QKGLQEGFSNWVDNASDYASQAAQLATEGISGMVNNITEMLNGNKV"
BASE COUNT          625 a          593 c          846 g          472 t
ORIGIN      
        1 gcggttctgc tgtcggaatg gctggcgcat ttgccggtgc ctttgcctcc ggacacctga
       61 tttcactggc ggatgagtgg agttccgtaa atgcccgtct gaaacaggca tcacagtcat
      121 ccgatgaatt ttcgtcatca cagaaagtgc tgatggatat cagccagcgg acaggcacgg
      181 cattttcgga taatgcggcc ctgtttgccc gttcggctgc ctcgatgcgt gaatatggtt
      241 acagtgccgg tgatgtactg aaggtgacgg aggccatttc cacggggctg aaaatctccg
      301 gtgccagtac ggctgaggcg ggttcggtga tcacccagtt cagccaggcg ctggcacagg
      361 gtgtgttgcg cggtgaggaa tttaattcgg tcaatgaaag tggtgaccgg atcgtacgtg
      421 cactggctgc gggtatgggc gtggcccgta aagatctgaa ggcaatggcg gatgacggaa
      481 aactgacagc ggataaagtg gtccccgcgt taatcagcca gctggggata ttacgtgatg
      541 aatatgcggc catgccggaa acggtttcca gtagtatcac gaaggtggaa aacgccttta
      601 tggcctgggt gggcggtgcg aatgaggcca gcggggtgac aaaaacgctc tccggcatgc
      661 tgaacggtgt tgccggacag attgataatg tggcaacagc cgtgggcgcg ctggttgccg
      721 tcggggttgc acgttacttt ggcaatatgg cctccggagc gatgtctgcc acggcaggac
      781 ttgtgacggc tgcacgtaat gaagttgcac tggcggaagc acagttcagg ggaacgcaga
      841 ttgccacggc gcgggcaagg gcagccgtgt accgtgctca gcaggccgtg gcggcagccc
      901 gcgggacgga gatgcagatt gcagcagagg cccgtctggc ggccacacag gaacgcctga
      961 acagaaatat tgctgccaga accgccgccc agaatgcgct gaacagtaca acggcggtgg
     1021 gctcacgtct gatgagcggt gcgctggggc tggttggtgg cgtacccgga ctggtgatgc
     1081 tgggggctgc agcatggtac acgctgtacc agaatcagga gcaggccagg gagtctgcgc
     1141 gccagtatgc actgacgata gatgaaatcg cgcataaaac gccgtcaatg tctctgcctg
     1201 aagcctcaga taatgaagga cgaacacggg cggcgctgac agagcagaac cggctgattg
     1261 atgaacaggc cagtcgggtg aaatccctgc aggaaaaaat cgcaggatat cagtatgttc
     1321 tggcgaaccc gggctggacg accggtgacg gattcatgat aaaccatctg acatcggtga
     1381 agaccgtaac ggaagggctt gctcaggcaa cagagcagct tgccgttgag cagtcccgtc
     1441 tggcacagat gcaggaaaaa gcgcagtcca ttcaggatgt gcttgccggg ctggaagacc
     1501 gtcgtgtggc gttaattcgt cagcaggcgg cagagcagaa taaggtgtac cagtccatgc
     1561 tggttatgaa cggtcagtat acggaattca accgtctgct ggggctgggg aatgaactgc
     1621 ttcagcagcg gcagggactg gtgaatgtgc cgttacggct gccacaggcc actctggatg
     1681 ataaacagca gagtgccctg acaaaaacag agcgtgagct ggccctgtcc agactgaaag
     1741 gggaagaaaa agagcgcgtc cgactggggt atgcggcgga tgacctcggt tttgtggggg
     1801 atccgtatca ggaggcgaga caacgttata tcagtaatgc cctggaagcc tggcgcaata
     1861 acgaggtgaa taaacccaaa tcccggggtg gaaaatcaga gacggaaaaa gcggaagaca
     1921 gtttttcccg gctgctgaag cagcagaaag agcaactggc actggtgggg cagaatacag
     1981 agctggcgaa gctgaaatac cagacagcgc tgggtgaact gaaaaccctg acggagatgc
     2041 agaagcagga actgctgcgt aacgcgaccc tgattgacca gcaaaaaatc cgggaacagt
     2101 tgcgatcccg ggaagagaca ctgaagaatg agaatgcggc tgcgcgtgcg tcgaatgatg
     2161 ctgaactgct ggggtacggg cagggggagc gagccagaga acgcatgcgg gagttgcagc
     2221 agatccgcga cagcttccgc cagaaggatg cggaccttca gtctcagtat cagaccgggg
     2281 atatcagtga ggatttttac agacaggctc tggcacagaa cgcgcagtat ctgagcgaac
     2341 gccttaagga ccaggcagtc ttttatgccg aatcggatgt gcagcgtgcg gactggcaga
     2401 aagggctgca ggagggattc agtaactggg tggataatgc gtccgattac gcctcacagg
     2461 cagcacagct ggcgacggag ggtatctcag ggatggtgaa taacatcacg gagatgctga
     2521 acggaaataa agtgga
//
LOCUS       DBFIFI010000348         2524 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.350, whole genome shotgun sequence.
ACCESSION   DBFIFI010000348 DBFIFI010000000
VERSION     DBFIFI010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.350"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..1962
                     /locus_tag="N7B39_004806"
     CDS             <1..1962
                     /locus_tag="N7B39_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HGU2424613.1"
                     /translation="LTLDREVTLPETGAATVNLINGSGKPVSVDITAHPAPDRIQVST
                     LPDGVETYGVWGLSLPSLRRRLFRCVSVRENTDGTFAITAVQHVPEKEAIVDNGARFE
                     PQSGTLNSVIPPAVQHLTVEVSAADGQYLAQVKWDTPRVVKGVRFSLRLTSGSGEGSR
                     LVTTAITADTEHRSSGLPPGEYTLTVRAINSYGQQGEPATTTFRINAPAVPATIELTP
                     GYFQITAVPRLAVYDPTVQFEFWFSETKIADTSQVETSARYLGTGSQWTVQGSRIKPG
                     TDFWFYVRSVNLVGKSAFVEVSGQPSNDGEGYLEFFREKIGKLHLAQGLWELIDNSQL
                     ADEMAEMKTTITETRNEITQTVSKTLENQSAIIQQIQRVQKDTNDDLAALYMLKVQKT
                     KNGIPYVAGIGAGIEDTDGQPLSNILLLADRIAMINPEDGNTTPLFVAQGNQLFMNDV
                     FLKRLFAVSITSSANPPTFSLTPEGRLTARNADISGNVNANSGTLNNVTINENCRVLG
                     KLSANQIEGDLVKTVGKAFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFSGA
                     KHEREHTDIYSSCRLIVRKNGAEIYNRTALDNTLIYSGVIDMPAGHGHMTLEFSVSAW
                     LVNNWYPTASISDLLVVVMKKATAGISIS"
     gene            2030..>2524
                     /locus_tag="N7B39_004807"
     CDS             2030..>2524
                     /locus_tag="N7B39_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HGU2424614.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHVSTNVPG
                     SDELNGINVKYRYEFTDTLGMVTSFSYAGDKNRQLTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQ"
BASE COUNT          595 a          638 c          763 g          528 t
ORIGIN      
        1 ctgacgctgg accgtgaggt gacactgccg gagaccggtg ccgccacggt gaacctgatt
       61 aacggcagcg gtaagccggt gagtgtggac atcaccgcac accccgcgcc ggaccggata
      121 caggtcagca ccctgcctga tggtgtggag acatacggtg tatggggact atccctgccg
      181 tcactgcgcc gtcgcctgtt ccgctgtgtc tccgtccggg aaaacacgga cggcaccttt
      241 gccatcacgg cggtgcagca cgtaccggaa aaagaagcca tcgtggataa cggtgcccgc
      301 tttgagccgc agtcaggcac cctgaacagc gtcatccctc cggcagtgca gcacctgacg
      361 gtggaggtga gcgcagctga cggccagtat ctggcgcagg tgaaatggga cacgccgcgg
      421 gtggtgaagg gcgtgcgctt cagtctgcgc ctgaccagcg gaagcggaga aggcagccgt
      481 ctggtgacca ccgccatcac tgcggataca gagcatcgtt ccagtggtct gccgccgggg
      541 gaatacaccc tgacggtcag ggcgattaac agttatggcc agcaggggga accggccacc
      601 accacgttca ggattaatgc acctgcggta cccgccacga ttgagctgac accgggctat
      661 tttcagataa cagcggtccc gcgtcttgcg gtgtatgacc cgacggtaca gtttgagttc
      721 tggttttcgg agacaaaaat cgcagataca tctcaggtgg aaacctctgc ccgttatctg
      781 gggaccggca gtcagtggac tgtccaggga agccggatta agccggggac ggatttctgg
      841 ttttacgtgc gcagcgtcaa cctggtgggg aaatctgcgt ttgtggaagt cagcgggcag
      901 cccagcaatg atggtgaagg gtatctggaa tttttccggg aaaaaatagg aaaactgcat
      961 ctggctcagg ggctatggga gctgatagac aacagccagc ttgcggatga gatggcggag
     1021 atgaagacca ccatcacgga aacccgcaat gaaatcacac agacggtcag taaaacgctg
     1081 gagaaccaga gcgccatcat acagcagata cagcgcgtgc agaaggacac aaatgatgac
     1141 cttgctgcac tttacatgct gaaggtacag aaaacaaaaa atggcatacc ctatgttgcc
     1201 ggtattggag cggggattga ggatactgat ggccagcccc tgagcaacat actgctgctg
     1261 gctgaccgta ttgcgatgat taacccggag gacggcaaca ccacgccgtt atttgtggcg
     1321 caggggaatc agttgttcat gaacgatgtg ttcctgaaac gactgtttgc ggtgagtatc
     1381 acgtcatccg ccaatccccc gacgttttcc ctgacgccgg agggcaggct gaccgcaaga
     1441 aatgctgata tcagcggtaa cgtgaatgcg aattccggga cgctcaacaa cgtcacgatt
     1501 aacgagaact gtcgggttct gggaaaactg tccgcgaacc agattgaagg cgatctcgtt
     1561 aaaacagtgg gcaaagcttt cccccgggat tcccgtgcac cggagcggtg gccatcaggg
     1621 accattaccg tcagggttta tgacgatcag ccgtttgacc ggcagattgt tattccggcg
     1681 gtggcattca gcggcgctaa acatgagaga gagcatactg atatttactc ctcatgccgt
     1741 ctgatagtgc ggaaaaacgg tgctgaaatt tataaccgta ccgcgctgga taatacgctg
     1801 atttacagtg gcgttattga tatgcctgcc ggtcacggtc acatgacgct ggagttttcg
     1861 gtgtcagcat ggctggtgaa taactggtat cccacagcaa gtatcagcga tttgctggtt
     1921 gtggtgatga agaaagccac cgcaggcatc agtatcagct gaattttata acccatatac
     1981 gggcgccaga aatggcgcct tttttattgc agaaaagcga gaggtaatta tgcgtaaact
     2041 ttatgccgcc attttgtccg cagccatttg tctggccgta tccggtgcgc ctgcatgggc
     2101 gtctgaacat cagtcaacgc tgagcgcggg gtatcttcat gtctcgacga acgttcctgg
     2161 cagcgatgaa ctgaacggga ttaacgtgaa ataccgttat gagtttacgg acacactggg
     2221 gatggtgacg tcgttcagct atgcaggaga caagaatcgc cagctgaccc gttacagcga
     2281 tacccgctgg catgaagatt ccgttcgtaa ccgctggttc agcgtaatgg cggggccgtc
     2341 tgtgcgcgtg aatgaatggt tcagcgcgta tgcgatggcg ggtgtggctt acagccgtgt
     2401 gtcgactttc tccggggatt atctccgcgt aactgacaac aaggggaaaa cgcatgatgt
     2461 gctgaccgga agtgatgacg gtcgccacag caacacgtct ctggcgtggg gagctggcgt
     2521 gcag
//
LOCUS       DBFIFI010000349         2503 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.351, whole genome shotgun sequence.
ACCESSION   DBFIFI010000349 DBFIFI010000000
VERSION     DBFIFI010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.351"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            101..2368
                     /gene="adiA"
                     /locus_tag="N7B39_004808"
     CDS             101..2368
                     /gene="adiA"
                     /locus_tag="N7B39_004808"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HGU2424615.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFAADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
BASE COUNT          628 a          684 c          619 g          572 t
ORIGIN      
        1 aatatttcaa aaatgtttgt ttttcacgcg ctttacagcc cgaaaaggcc ggaagatact
       61 tgcccgcaac gaagattcct tcataaccgg gtaagcaatg atgaaagtat taattgttga
      121 aagcgagttt ctccatcaag acacctgggt cggtaacgct gttgagcgtc tggcagatgc
      181 tttaagccag caaaatgtta ccgtgattaa atccacctcc tttgatgatg gttttgccat
      241 tctctcttca aacgaagcca ttgactgcct gatgttcagc tatcaaatgg aacatccgga
      301 cgaacatcaa aacgtcagac aattgatcgg taagcttcat gagcgccaac aaaacgtgcc
      361 ggtcttcctg ttgggcgatc gggaaaaagc cctcgccgca atggaccggg atctgctgga
      421 gcttgtcgat gaattcgcct ggattctgga agataccgcc gactttatcg ccggacgcgc
      481 cgttgccgcg atgacccgct accgccagca gctgttgccg ccactgttca gcgcgctgat
      541 gaaatatagc gacatccatg aatattcctg ggcagcgcca ggccaccagg gcggcgttgg
      601 ttttaccaaa acacccgccg gacgtttcta ccatgactac tacggtgaaa atctgttccg
      661 caccgacatg ggcatcgaac gaacttccct cggttctttg cttgaccata ctggcgcatt
      721 tggcgaaagc gaaaaatatg ccgcacgcgt atttggtgcc gatcgctcct ggtcggtagt
      781 cgtcggtact tccggctcta accgcaccat catgcaggct tgcatgaccg ataacgatgt
      841 cgtggtcgtt gaccgtaact gccataaatc catcgaacaa ggtttgatgc tgacaggcgc
      901 gaaaccggtc tacatggtgc caagccgcaa ccgctacggc attatcgggc caatctatcc
      961 gcaggaaatg caacctgaaa ccttgcagaa gaaaatcagt gaaagcccgc tgactaaaga
     1021 caaagccggg caaaaaccgt cttactgcgt ggtgaccaac tgcacctatg acggcgtgtg
     1081 ttataacgct aaagaagcgc aggatctgct ggaaaaaacc tccgatcgtc tgcactttga
     1141 cgaagcctgg tacggctatg cacgtttcaa cccgatctat gccgatcact atgccatgcg
     1201 cggcgaacct ggcgatcaca acggtcctac cgttttcgcc acccactcca cccacaaact
     1261 gctgaatgcg ctttcacagg cctcttatat tcatgtacgt gaaggtcgtg gggcgattaa
     1321 cttctcccgc ttcaaccagg cctacatgat gcatgccacc acctccccgc tgtatgccat
     1381 ctgcgcatcc aacgacgtgg cggtgtcgat gatggacggc aacagcggcc tgtcactgac
     1441 gcaggaagtg atcgacgaag cggttgattt ccgtcaggcg atggcgcggc tgtataaaga
     1501 gttcgccgct gacggtagct ggttcttcaa accgtggaac aaagaagtcg tcaccgaccc
     1561 acaaaccggc aaaacctatg actttgctga cgcaccaacc aaactgctga ccaccgttca
     1621 ggactgctgg gtaatgcatc cgggcgaaag ctggcacggc ttcaaagata ttccggataa
     1681 ctggagtatg ctcgacccga ttaaagtcag catccttgct ccgggaatgg gtgaagatgg
     1741 tgaactggaa gaaaccggtg ttccggcggc gctggtcact gcctggcttg gtcgccacgg
     1801 cattgtgcct acccgcacca ctgacttcca aattatgttc ctgttctcta tgggcgtaac
     1861 ccgtgggaaa tgggggactc tggttaacac cctttgctcc ttcaaacgcc actatgacgc
     1921 caacacaccg ctggcgcagg tgatgccgga acttgttgaa caatatcctg acacttacgc
     1981 gaacatgggg attcacgatc tgggtgacac catgtttgcc tggctgaaag aaaacaaccc
     2041 tggcgcacgg ttgaacgaag cctattccgg cctgccggtg gcggaaatca ccccgcgtga
     2101 agcgtacaac gcgattgtcg acaacaatgt cgaactggta tccattgaaa atctgccagg
     2161 acgtatcgcg gcaaactcag ttatcccgta tccgccagga atcccgatgc tgctgtctgg
     2221 tgaaaacttc ggcgataaaa acagtccgca agtaagttat ttacgctcgc tgcaatcctg
     2281 ggaccatcat ttccctggat ttgaacacga aactgaaggg actgaaatta ttgacggtat
     2341 ttaccacgtt atgtgtgtga aagcgtaacc actattccgc tgaaggcgta attgtttaaa
     2401 taacattacg ccgcctggcc ttaggccttt tgagtatggc aacgttttca taaaaattgc
     2461 tgcaaacaaa aatgtcatac tttttgcgcg gccccacccc gcg
//
LOCUS       DBFIFI010000350         2500 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.352, whole genome shotgun sequence.
ACCESSION   DBFIFI010000350 DBFIFI010000000
VERSION     DBFIFI010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.352"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            551..742
                     /locus_tag="N7B39_004809"
     CDS             551..742
                     /locus_tag="N7B39_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HGU2424616.1"
                     /translation="MSEDVPLPKVNQRYKDDHGALVTLTSVEETRVVFMQDGYPHPCM
                     RPMYNFLGKFKPEPREETE"
     gene            complement(795..1028)
                     /locus_tag="N7B39_004810"
     CDS             complement(795..1028)
                     /locus_tag="N7B39_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424617.1"
                     /translation="MARSDYDIINLSLEHELNEWLAERGYAGLVDNRNRLAEVVTRKL
                     QDSFYINVSRDALNTAYSEHPEWFSGLVSGDEN"
     gene            complement(1124..1747)
                     /locus_tag="N7B39_004811"
     CDS             complement(1124..1747)
                     /locus_tag="N7B39_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGU2424618.1"
                     /translation="MRTRIDYLADKYCFTERNESPRLRRQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWGISQKNVVFNDKRFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRVVGNENTSLPYQQVAREVKAPRERLKYALEAGLLVTALD
                     GLFRFGSQRIAADVLRLRKAGMPVVTTTVEVHDNLTGTTRKVPAYHL"
     gene            complement(1836..2345)
                     /locus_tag="N7B39_004812"
     CDS             complement(1836..2345)
                     /locus_tag="N7B39_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424619.1"
                     /translation="MQITEALISEPGEIRRFVQQAVDHWPNLLAFHFTLYSAEGIYGQ
                     QIQTFCSSFHRRVHERITEHNHTVSPSAPVVLRWLREQHEGAQIRCLLLLSQTSICHP
                     RVGVMADEECAQLVDLLQQTWSVISAGGQCRVERCFRVARPGSSGQYVALKTAVQSFM
                     SQVIATIIR"
BASE COUNT          725 a          643 c          526 g          606 t
ORIGIN      
        1 cagcctcctg acgatacctc ccaccatgtt ttgcgatggc acgctaccgc ccttccggta
       61 tttacccgca tcaaccggta ccagaatgct gagtaacaaa ccagctagaa ttgacttaat
      121 tttgatcatt atagaataat gatctgaact acagcaacat gacagattta tcgatcgata
      181 taacacaaat gaacagttct ttattctgac aaatatcgca aaaacagcac attacaaaat
      241 ttataaccat aattactgga aaagtgcaga aaattattcc actgaatgct tttaatacag
      301 ttggttagct taaaaaatag caatttttga tttcagtcat tgaaagttgc aatgtttgac
      361 gattcaacag catttaagtg agtttcagga tatgggaagg acatgaaagg aggagcgcaa
      421 taaaatacac aggaagaagg ggaaaatgtt tcgtaaaaac agaaaattaa cagcgcacat
      481 caggaaacgc agtctcctga ttcagtgcta ccacatgaaa atttagttaa ctgttaccgt
      541 actaaaattt atgtcagaag atgttccatt accgaaagtc aatcagcgat acaaagacga
      601 tcatggtgcg cttgtcaccc tgaccagtgt tgaggaaacc cgggtggtgt ttatgcagga
      661 cggctatccg catccctgca tgcgaccgat gtacaacttt ctgggaaaat tcaaacccga
      721 accccgggaa gaaacagaat aaaacgaaat ccctgccggc aatcacacta cctgatagca
      781 gcataatcca cttattaatt ctcatccccg gagacaagcc cagaaaacca ctccgggtgt
      841 tcactgtatg ccgtgttcag cgcatccctg gaaacgttta tataaaagct gtcctgcaat
      901 ttgcgggtaa caacttctgc caggcgattc cggttatcca ccagtccggc ataacctctc
      961 tctgccagcc actcattcag ttcatgttcc agagacagat tgataatatc ataatcggac
     1021 cttgccattc ccgtttcctt agcacatgtt ttcaggcacg gggataaagt ataaagaatg
     1081 tcatactgct gctgccagag caatattccc cctgatggtt gtatcagaga tggtatgccg
     1141 gtactttgcg ggttgttccc gtcaggttat cgtgcacctc cacggtcgtg gtcaccaccg
     1201 gcattccggc ctttctcagc ctcagaacat cagccgcaat acgctgactg ccaaacctga
     1261 acaggccgtc cagtgcagtc accagtaaac ccgcctccag tgcatacttc agccgttcac
     1321 ggggcgcttt cacttcccgg gcaacctgct gatatggcaa tgatgtgttt tcattcccaa
     1381 ccacgcgacg aatacggtga gacagatgat accggtatgt atccggcaca ccagaaaggc
     1441 tggccttcag gctgtacacg cagccaaacc gtttatcgtt aaacaccaca tttttctggc
     1501 tgatgcccca ttcttcacgc agcgcggcaa tcaattgcgg tgtacgggta agcagcaagc
     1561 gaaaaggcag ttcaaaactg gtgacgtaat ccacattcag cagtgcaata cgcaaccgtt
     1621 cttctggccc ggcctctgtc tgccggcact cctccagaac atcctgccac tgccggcgaa
     1681 gacgggggga ttcgttccgt tctgtaaaac agtatttatc cgccagatag tcaattcgtg
     1741 tacgcatact gaaaggaatt ccgtataaag attcagcaga caaaacttta tcagccagta
     1801 aaaattaacg gaagcattga tattcctccc gacaatcacc ggatgattgt tgcaataacc
     1861 tgcgacataa aggactgaac agccgttttt aacgccacgt attgccctga tgaaccaggc
     1921 cgggccaccc gaaaacatct ttccacccgg cattgtccgc cagcacttat caccgaccag
     1981 gtctgttgca gtaaatccac cagttgcgca cattcttcat cagccatgac gccgactcgc
     2041 ggatggcaaa tactcgtctg gctgagcagc aacagacatc gtatctgtgc tccttcatgt
     2101 tgttcccgca accagcgtaa taccaccggc gctgatgggc tgaccgtgtg attatgctcc
     2161 gtaatacgtt cgtgtacccg tcggtgaaag gaactacaaa atgtctgaat ctgttgacca
     2221 tagattcctt ctgccgaata gagcgtgaag tgaaatgcca acagatttgg ccagtggtcc
     2281 accgcctgct gaacaaaacg ccggatttcc cccggctctg aaattaaggc ttcggttatt
     2341 tgcacgaaat gttccttttc aacccctgtt gaattttgtc aagccggtgc agacacagta
     2401 aagcgcgcat caacacctga aaggtacgat acaaacacaa gttacaaaca ataagaacgg
     2461 aaggacaatt aatcagtgag gaataataac taaattaacc
//
LOCUS       DBFIFI010000351         2466 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.353, whole genome shotgun sequence.
ACCESSION   DBFIFI010000351 DBFIFI010000000
VERSION     DBFIFI010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.353"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(1..1658)
                     /locus_tag="N7B39_004813"
                     /pseudo
     CDS             complement(1..1658)
                     /locus_tag="N7B39_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309571.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(1604..1825)
                     /locus_tag="N7B39_004814"
     CDS             complement(1604..1825)
                     /locus_tag="N7B39_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424620.1"
                     /translation="MAKIQYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITARRPA
                     GQKKKRDAEHGECGTFCGDPEKTRNQDVM"
     gene            complement(1870..>2466)
                     /locus_tag="N7B39_004815"
     CDS             complement(1870..>2466)
                     /locus_tag="N7B39_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HGU2424621.1"
                     /translation="ALAEAVVARLDTDFVDPKKAAVADVSPASITHDVKGTASSGNPD
                     ADAEAAFGQFVTANLQPTGAVWLMSSTNALALSMRKNALGQKEYPDMTLLGGTFQGLP
                     VIVSQYVGDQLVLVNAPDIYLADDGGVAVDMSREASLEMQSEPTSDSSTPSPVELVSM
                     FQTGSVAIRAERWINWRRRRTAAVAVITGVNYGTASGG"
BASE COUNT          500 a          813 c          603 g          550 t
ORIGIN      
        1 cggcagggca ctcaccgcat cattcaccgc cttctgcaga acatccggat cgtagtcacg
       61 accgtcgcgc ggaacaggaa tatggctcac tgcctctttc accatctgct caagcatcgg
      121 acgcacatca tcacccgtca cgcacttctg taataccaca gactgagaag ccagtttttc
      181 ttcaaacgtt tgtgcctgcg cggctatttt ctcctcaaat gtacgctgta aatccgccag
      241 caccatggcg aattcttcgc ccagtgcacg aataatggac agttcccgtt ccgtcatttt
      301 cgcagtatcc ccctgaacat cgctttcacc gcatcatgct ctgtttcact gattgcctta
      361 ttaccgtcag atgcgccatc aggcagttgt gctgagactg ttttcccgga cgacgcgaac
      421 ggatcctcac gggcatcacg acgggacagc gcctccagac tgtagttctg ctgctgaaga
      481 tacagtgcat caccgccggc caggggcggc aggttctcac gtttacgggc ctcattgggc
      541 gtcagcagcg tatttttcac cgactcaccc agcgttttca tgcgccgttc gctgtccatt
      601 ctcagcagcg tggtgacgtc aaactccgtg ctctcgtttt cccccgtttc cagcgcctca
      661 tccagtaaca gctcaatgga ctcaatcagc gtctgcagac actgggaata atactgctgc
      721 tccagcgcct ccacgttatc actggaggga ggttgtccca caccaatctt gtaggccggg
      781 acacggaaca ccgaacagac aatttcagcg gtcattttca gttgttccac cgtctgcgca
      841 tccaccggtg aaaacgtcgt ggggttatat ttcgccccgt tgctcagaat cgccgttttc
      901 cccgcatttt cgccggtata cccgctgtcc cagttgctct tcagtttttt cgcattttct
      961 tccgttatac tgccggggat ctcaatcacc ccggacggcc tgccgccatt tctgaaaaaa
     1021 gacgttgaat ttgcctgaat atgatacccc tgcgtggcag ccagcccggc agcatacacc
     1081 ggcggcagcc ccacaagcgg atgaaaaaaa cagttaaacc ggtcgtggat cacttcccgg
     1141 gcaggcaccg tcaccgcctc cgtgatcccg cagttccggt ccggcgtgat gcgatagaac
     1201 acctcgccgt catccgccac cagaggttca acccggctcc agtccagaat acgcagttct
     1261 ttgatctgcc cacgggagtt acggattttc agcaccaccg tattgccgtg acgcagtttg
     1321 gcgttcagcc acagttcaaa aaactggata cgattctgct gtgcattggg acgacgacag
     1381 agacgggcaa tatccccctg ccgtttttca cggcggatcc cctgtgtatc ggtctgcatc
     1441 aggcgcagcc gcattttggc gatatcctgg gatatcagcg aaatgcaaga aaacaccgca
     1501 tgaaaggaga ggacactttc cggatcggct ttcactccct gctgccaggc accggcaaaa
     1561 ggctcagcca ccgcctgaaa caggctggtc cagcccacct cttttacatc acgtcctgat
     1621 ttctggtttt ttcggggtcg ccgcaaaagg ttccacattc gccatgctcc gcatcacgtt
     1681 tctttttctg acctgccgga cgtcgcgctg tgatgtactc cgccttcccc aggcgaacca
     1741 gcacctccgc acacggctgt gccacatcac ggatatcccc ggcccgggca tcatgcgtgc
     1801 cctgcagata ctggattttt gccatcagtt actgcgggaa gctagcgcct cccgccctcc
     1861 tcatcagact cagccgccgg acgcagttcc gtagttcaca ccggtgatca cagccaccgc
     1921 cgcggtacgg cgacgacgcc agttgatcca gcgctccgca cggatggcca cgctgccggt
     1981 ctggaacatg gagaccagct ccaccggtga cggtgtgctg ctgtcgctgg tcggttcaga
     2041 ctgcatctcc agtgacgctt cacgggacat atccaccgcc acaccaccgt catccgccag
     2101 ataaatatcc ggcgcattca ccagtaccag ctggtcaccc acatactggg agacaatcac
     2161 cggcagcccc tggaaggtcc cgcccagcag ggtcatgtcc ggatattcct tctgccccag
     2221 cgcattttta cgcatggaca gcgccagggc attcgtgctg gacatcagcc agaccgcacc
     2281 ggtgggctgc agatttgccg tcacaaactg gccaaacgcg gcctcggcat ccgcatccgg
     2341 attaccgctt gacgccgtgc ccttcacatc atgggtgatg gacgcagggg agacatctgc
     2401 caccgccgct tttttcgggt ccacaaagtc tgtatccaga cgcgccacca ccgcttccgc
     2461 cagcgc
//
LOCUS       DBFIFI010000352         2459 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.354, whole genome shotgun sequence.
ACCESSION   DBFIFI010000352 DBFIFI010000000
VERSION     DBFIFI010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.354"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..1171
                     /locus_tag="N7B39_004816"
     CDS             <1..1171
                     /locus_tag="N7B39_004816"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HGU2424622.1"
                     /translation="AIAISAFHALKRNPQEDPLLLVLAADHVIAKESVFCDAIKNATP
                     IANQGKIVTFGIIPEYAETGYGYIERGELSVPLQGHENTGFYYVNKFVEKPNRETAEL
                     YMTSGNHYWNSGIFMFKASVYLEELRKFRPDIYNVCEQVASSSYIDLDFIRLSKEQFQ
                     DCPAESIDFAVMEKTEKCVVCPVDIGWSDVGSWQSLWDISLKSKTGDVCKGDILTYDT
                     KNNYIYSESALVAAIGIEDMVIVQTKDAVLVSKKSDVQHVKKIVEMLKLQQRTEYISH
                     REVFRPWGKFDSIDQGERYKVKKIIVKPGEGLSLRMHHHRSEHWIVLSGTAKVTLGDK
                     TKLVTANESIYIPLGAAYSLENPGIIPLNLIEVSSGDYLGEDDIIRQKERYKHED"
     gene            1175..>2459
                     /gene="cpsG"
                     /locus_tag="N7B39_004817"
                     /gene_synonym="manB"
     CDS             1175..>2459
                     /gene="cpsG"
                     /locus_tag="N7B39_004817"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HGU2424623.1"
                     /translation="MKSLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSEALKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGEMVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEP"
BASE COUNT          687 a          531 c          611 g          630 t
ORIGIN      
        1 agcaatagcg atatctgcgt ttcatgcgtt aaaacgtaat cctcaggaag atccattgct
       61 tctagttctt gcggcagacc acgttatagc taaagaaagt gttttctgtg atgctattaa
      121 aaatgcaact cccatcgcta atcaaggtaa aattgtaacg tttggaatta taccagaata
      181 tgctgaaact ggttatgggt atattgagag aggtgaacta tctgtaccgc ttcaagggca
      241 tgaaaatact ggtttttatt atgtaaataa gtttgtcgaa aagcctaatc gtgaaaccgc
      301 agaattgtat atgacttctg gtaatcacta ttggaatagt ggaatattca tgtttaaggc
      361 atctgtttat cttgaggaat tgagaaaatt tagacctgac atttacaatg tttgtgaaca
      421 ggttgcctca tcctcataca ttgatctaga ttttattcga ttatcaaaag aacaatttca
      481 agattgtcct gctgaatcta ttgattttgc tgtaatggaa aaaacagaaa aatgtgttgt
      541 atgccctgtt gatattggtt ggagtgacgt tggatcttgg caatcgttat gggacattag
      601 tctaaaatcg aaaacaggag atgtatgtaa aggtgatata ttaacctatg atactaagaa
      661 taattatatc tactctgagt cagcgttggt agccgccatt ggaattgaag atatggttat
      721 cgtgcaaact aaagatgccg ttcttgtgtc taaaaagagt gatgtacagc atgtaaaaaa
      781 aatagtcgaa atgcttaaat tgcagcaacg tacagagtat attagtcatc gtgaagtttt
      841 ccgaccatgg ggaaaatttg attcgattga ccaaggtgag cgatacaaag tcaagaaaat
      901 tattgtgaaa cctggtgagg ggctttcttt aaggatgcat caccatcgtt ctgaacattg
      961 gatcgtgctt tctggtacag caaaagtaac ccttggcgat aaaactaaac tagtcaccgc
     1021 aaatgaatcg atatacattc cccttggcgc agcgtatagt cttgagaatc cgggcataat
     1081 ccctcttaat cttattgaag tcagttcagg ggattatttg ggagaggatg atattataag
     1141 acagaaagaa cgttacaaac atgaagatta acatatgaaa tctttaacct gctttaaagc
     1201 ctatgatatt cgcgggaaat taggcgaaga actgaatgaa gatattgcct ggcgcattgg
     1261 gcgtgcctat ggcgaatttc tcaaaccgaa aaccattgtt ttaggcggtg atgtccgcct
     1321 caccagcgaa gcgttaaaac tggcgcttgc gaaaggttta caggatgcgg gcgtcgatgt
     1381 gctggatatc ggtatgtccg gcaccgaaga gatctatttc gccacgttcc atctcggagt
     1441 ggatggcggc atcgaagtta ccgccagcca taacccgatg gattacaacg gcatgaagct
     1501 ggtgcgcgaa ggggctcgcc cgatcagcgg tgataccgga ctgcgcgatg tccagcgtct
     1561 ggcagaagcc aatgacttcc ctcctgtcga tgaaaccaaa cgtggtcgct atcagcaaat
     1621 caatctgcgt gacgcttacg ttgatcacct gttcggttat atcaacgtca aaaacctcac
     1681 gccgctcaag ctggtgatca actccgggaa cggcgcagcg ggtccggtgg tggacgccat
     1741 tgaagcccga tttaaagccc tcggcgcacc ggtggaatta atcaaagtac acaacacgcc
     1801 ggacggcaat ttccccaacg gtattcctaa cccgctgctg ccggaatgcc gcgacgacac
     1861 ccgtaatgcg gtcatcaaac acggcgcgga tatgggcatt gcctttgatg gcgattttga
     1921 ccgctgtttc ctgtttgacg aaaaagggca gtttatcgag ggctactaca ttgtcggcct
     1981 gctggcagaa gcgttcctcg aaaaaaatcc cggcgcgaag atcatccacg atccacgtct
     2041 ctcctggaac accgttgatg tggtgactgc cgcaggcggc accccggtaa tgtcgaaaac
     2101 cggacacgcc tttattaaag aacgtatgcg caaggaagac gccatctacg gtggcgaaat
     2161 gagcgctcac cattacttcc gtgatttcgc ttactgcgac agcggcatga tcccgtggct
     2221 gctggtcgcc gaactggtgt gcctgaaagg aaaaacgctg ggcgaaatgg tgcgcgaccg
     2281 gatggcggcg tttccggcaa gcggtgagat caacagcaaa ctggcgcaac ccgttgaggc
     2341 aattaatcgc gtggaacagc attttagccg cgaggcgctg gcggtggatc gcaccgatgg
     2401 catcagcatg acctttgccg actggcgctt taacctgcgc tcctccaaca ccgaaccgg
//
LOCUS       DBFIFI010000353         2446 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.355, whole genome shotgun sequence.
ACCESSION   DBFIFI010000353 DBFIFI010000000
VERSION     DBFIFI010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.355"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..998
                     /locus_tag="N7B39_004818"
     CDS             <1..998
                     /locus_tag="N7B39_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HGU2424624.1"
                     /translation="SFNGWEIFNSNFERMIKENKTMLLCKWGFYLTCVVAVMFVFAAI
                     TSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQAKGIEPLSIEKLQAL
                     QLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDIISKRRESLEEQWGIE
                     DSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVFPSDYISDCANHSSGK
                     SSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKAHELFESEEDYQKNSL
                     MGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIRNVPWGASSVKYS"
     gene            complement(1115..>2446)
                     /gene="gdhA"
                     /locus_tag="N7B39_004819"
     CDS             complement(1115..>2446)
                     /gene="gdhA"
                     /locus_tag="N7B39_004819"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HGU2424625.1"
                     /translation="YSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPKYRQM
                     SLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVNLSIL
                     KFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGADTDV
                     PAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYFTEAM
                     LKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTKEKLA
                     RLIEIKSSRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQLIAN
                     GVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLGWKAE
                     KVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
BASE COUNT          628 a          592 c          596 g          630 t
ORIGIN      
        1 attcttttaa tggctgggaa atatttaata gtaattttga acggatgatt aaagaaaata
       61 agaccatgct gctttgtaag tgggggtttt atttaacatg tgttgtcgct gtaatgtttg
      121 tattcgcagc gataacatcc aacggtttga atgaaagagg cctgattacc gcgggatgct
      181 cttttcttta tctattaatt atgatggggc ttattgttcg ggccggtttt aaagcaaaaa
      241 aagaacagct gcattattat caggctaaag gtattgagcc gctcagtatc gagaaattac
      301 aggcgctaca attgatcgca ccttatcgat tctatcataa gcaatggtct gaaacgctgg
      361 agttctggcc gcgaaagcct gaacctggca aagatacctt ccaatatcat gtgcttcctt
      421 ttgactcgat cgatatcata agtaaaagac gcgagtcttt agaggaacaa tggggtatcg
      481 aagatagcga aagttattgt gccttaatgg agcattttct ttctggcgac catggagcca
      541 atacctttaa agcaaacatg gaggaagccc cagagcaggt tatcgccttg ttgaataaat
      601 ttgctgtttt tccctcagac tatatctctg attgcgctaa tcatagctcc ggtaaatcct
      661 cggcgaagct aatatgggcg gcggaattat catggatgat ctcgatatca agcacagctt
      721 ttcaaaacgg gacaattgaa gaagaactgg cctggcatta tataatgctt gcttctcgaa
      781 aggcgcacga gttgttcgaa agcgaagaag attatcaaaa aaatagtcta atgggatttc
      841 tttactggca tatctgctgc tatcgcagaa agttaacgga tgctgaactt gaagcttgtt
      901 atcgttacga caagcagttt tgggagcact acagtaagaa atgccgttgg cccataagaa
      961 atgttccctg gggagcatca tccgttaaat actcataaac gcctgaaatt ttgccggggg
     1021 cgctgcgctt gcccggccta cattagagcg taactgcaat gaattgtgcc catttgtagg
     1081 cctgataagc gtagcgccat caggcattta caacttaaat cacaccctgc gccagcatcg
     1141 catcggcaac cttcacaaaa ccggcaatgt tcgcgccctg cacgtagttg gtttgctcac
     1201 cttcaccacc atgctcaaca caggcatggt ggatatccag catgatgtga tgcaaacgtg
     1261 cgtcaacttt ctcggctttc cagcccaggc gcgcagcgtt ttgtgccatt tccaggcccg
     1321 atgtagcgac gccaccagca ttagccgctt tacccggtgc aaatagtacg cctgcctgct
     1381 ggaacagttc agtcgcttcg atggtggtcg gcatatttgc cccttcggcg acggctttaa
     1441 cgccattagc gataagctga tgcgcggcgt caacatccag ttcgttctgg gtagcgcaag
     1501 gcagggcgat atcaaccggt acagaccacg gctgttggcc ttcgagatag accagaccaa
     1561 attctttggc gtaatctgcc actcgaccat cgcggctgga tttgatttcg ataagacgtg
     1621 ccagtttctc tttcgtgaat ccgctttcat caactacagt gccgctggag tctgacgcag
     1681 tgatcacacg agcaccaaat tccatcgctt tttcgatagc gtactgggcg acgttgccgg
     1741 agccagaaac ggaaacgcgc atgccttcaa aacccatacc gtggcgtttt agcattgctt
     1801 ctgtgaaata aaccagaccg tagccggtag cttccgggcg aataagactg ccgccaaatg
     1861 aaaggccctt accggtgaag acgcaggcgg tattgttgga gagctttttc atcatccccg
     1921 ccataaagcc gacttcacga ccaccaaccc cgatatcacc tgccggaacg tcggtatccg
     1981 cgcccaggtg gcgatacagt tcagtcatca gcgcctggca gaaacgcatc acttcgcctt
     2041 cgcttttccc tttcgggtcg aaatcgctgc cgcctttacc accgcccatc ggcagagtag
     2101 tcagggcatt tttgaaggtt tgttcgaagc cgaggaattt aagaatggaa aggttaactg
     2161 acggatggaa gcgcataccg cctttgtacg ggccgatggc agagctgaac tgcacacgcc
     2221 atgcacggtt gacctgtacc tgattgcgat catcaaccca taccacgcga aactggatca
     2281 cgcgctccgg ttcaaccaga cgctccagta atgacatctg gcgatatttt ggattttgtt
     2341 caagaaaagg ccagagtgtg gtcattactt cacgaacggc ttgcgcgaac tcggtttgat
     2401 tcgggtcgcg cttttggaca tggttgagga atgactccag agaata
//
LOCUS       DBFIFI010000354         2441 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.356, whole genome shotgun sequence.
ACCESSION   DBFIFI010000354 DBFIFI010000000
VERSION     DBFIFI010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.356"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..240
                     /locus_tag="N7B39_004820"
     CDS             <1..240
                     /locus_tag="N7B39_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HGU2424626.1"
                     /translation="TPTETDGALFPGRIMLANTCMWDYRGDECGYHGPAVADEFDNPT
                     TDIRKDRCSKCMRGCEMRGMVANFGGFLSINKLSQ"
     gene            complement(257..511)
                     /locus_tag="N7B39_004821"
     CDS             complement(257..511)
                     /locus_tag="N7B39_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HGU2424627.1"
                     /translation="MTKVRDIAPYSVRMPDSLKRDLTIRASKNGRSLNSEIVMILQAA
                     IDEEKSPRSIEGFAQQESEKFREALLKTLSSMYGEDKKPT"
     gene            621..731
                     /locus_tag="N7B39_004822"
     CDS             621..731
                     /locus_tag="N7B39_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HGU2424628.1"
                     /translation="MEKEVSRILVRIPQSLKDAITGKAKEECRSFNSEVM"
     gene            1034..1912
                     /locus_tag="N7B39_004823"
     CDS             1034..1912
                     /locus_tag="N7B39_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein/antirepressor Ant"
                     /protein_id="HGU2424629.1"
                     /translation="MKSIAKAQNDFTIFKFGDSEIRVINKCGEPWFVAKDVCDALALT
                     NSRKALTALDDDEKGVTLSYTLGGEQNLSIVSESGMYTLVLRCRDAVNKGSVPHKFRK
                     WVTAEVLPSIRKHGEYVKGKKTTVEERTPLRDAVNMLVGKKGLRNDDAYNMVHQRFGI
                     DSIDELSIEQIPLAVEYIHRVVLEGEFIGKQEKSTNELSAKEANSLVWLWDYANRSQA
                     LFRELYPALKQIQSNYSGRCYDYGHEFSYVIGMARDVLINHTRDVDINEPDGPTNLSA
                     WMRLKNKELPPSPHHY"
     gene            1966..>2441
                     /locus_tag="N7B39_004824"
     CDS             1966..>2441
                     /locus_tag="N7B39_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HGU2424630.1"
                     /translation="MLYSNILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPEDY
                     FRMSPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALSWWLVCRGEIHKFR
                     CVPHLTGRRFEHGVTDCYTLFRDAYHLAGIDMPDFEREDDWWRNGQNLYLDNMAVTG"
BASE COUNT          671 a          511 c          640 g          619 t
ORIGIN      
        1 acaccgacgg agacggacgg agcgctgttt cccggtcgca tcatgctggc gaacacctgt
       61 atgtgggatt accggggaga tgaatgcggt tatcacggtc ctgcggtggc ggatgagttc
      121 gacaacccca ccacggatat ccgtaaggac agatgcagca agtgcatgcg cgggtgtgag
      181 atgcgcggca tggtggctaa ttttggcggt ttcctttcca ttaataaact ttcgcagtaa
      241 tggattatgc ccaccatcag gtgggttttt tatcttcgcc gtacatgctg ctgagagtct
      301 taagaagagc ctccctaaat ttttcagatt cttgttgagc aaaaccttct attgatcttg
      361 gtgatttttc ttcatcaata gcagcttgca agatcataac tatttctgaa tttaatgagc
      421 gtccgttttt cgatgctctt atggtcaggt cgcgcttgag gctatctggc attcttacgc
      481 tatatggggc tatgtctcta accttcgtca tacttacacc gtgataatca cattgatatc
      541 acagtgtatt caaaaaaact ttgacttgat atagtcacag tgatacgttt gtgattcata
      601 ataactcacg cggtggcgat atggaaaaag aagttagcag aattttggtg agaatacctc
      661 agtcgctaaa agatgcgatt acaggaaagg ccaaagaaga atgccggtcg tttaactcgg
      721 aagtgatgtg attgctggac agcctgaaga gagaggggat aacggtatga gtaaagaatg
      781 ttgttttttg cggcattagc gaatcagacg ctgatcaaac atacatttac tctaaagaaa
      841 caggtcggat gctgtgtagt gactgcgtgt ttgacatcat aagatacaag tatcttggat
      901 gttctgccag cattagcaat ataggtgaag tgtacgaagg gaaagatata actgatagag
      961 cagaaagttg aagccccaac tgctgtaaca gtcagggctt cgttatcaac aaatcggctt
     1021 aggaaatatt gacatgaaaa gtatagcaaa ggcacaaaac gatttcacta tcttcaaatt
     1081 cggcgacagt gaaatccgcg tcatcaacaa gtgcggtgag ccgtggtttg tagctaaaga
     1141 tgtttgtgat gctttagctt tgactaactc acgcaaggcg cttactgcac ttgatgacga
     1201 tgaaaaggga gtaactttaa gttacaccct tggtggtgag cagaatctaa gcattgtgag
     1261 cgaatcaggt atgtatacat tggttctgcg ctgccgcgat gcagtcaata aaggttcagt
     1321 cccgcacaaa ttccgcaagt gggtaacagc agaagttctg ccttcaattc gcaaacatgg
     1381 cgagtatgta aaaggaaaga aaaccactgt tgaggaaaga acaccgctac gcgatgcagt
     1441 aaacatgctg gtaggaaaga aaggacttcg caatgacgat gcatacaata tggttcatca
     1501 gcgttttggt attgacagca ttgatgagct ttcaattgaa caaatcccgc ttgccgtaga
     1561 gtacatccac agggtagtgc ttgaaggtga attcattggc aaacaagaga agagcaccaa
     1621 cgagctttcc gcaaaagaag caaacagcct tgtatggcta tgggattatg ccaaccgctc
     1681 acaggcatta ttccgcgaac tgtatccggc gctaaaacaa attcaatcga actattccgg
     1741 cagatgctac gactacggtc atgagttctc gtatgttatt ggaatggcga gagacgtttt
     1801 aattaatcac acacgagatg ttgatatcaa tgagccagac ggaccaacga atctttccgc
     1861 atggatgaga cttaagaata aagaattacc tccttcaccg catcactact aacagattgc
     1921 caacgaaatg acccagcttc ggctgggttt ttatcaggag ttctcatgct ctatagcaat
     1981 atattggcgc acgcccggcg atgtgcacca gcggagtcgt gcggcttcgt ggtgagaacg
     2041 ccggaagggg aaagatattt tccctgcgtg aatatctccg gtgagccgga agattatttc
     2101 cggatgtcgc cggaggactg gctgcgggca gaaatgcagg gggagattgt ggcactggtc
     2161 cacagccacc ccggtggtct gccctggctg agcgaggccg accggcggct gcagataaaa
     2221 agtgcactgt cctggtggct ggtctgccgg ggggaaattc ataaattccg ctgtgtgcca
     2281 catctgacag gacggcgctt tgagcacggg gtgacggact gttacacgct gttccgggat
     2341 gcctaccatc tggcgggaat tgatatgccg gattttgagc gtgaggatga ctggtggcgc
     2401 aacggtcaga acctttacct ggacaatatg gcggtcaccg g
//
LOCUS       DBFIFI010000355         2406 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.357, whole genome shotgun sequence.
ACCESSION   DBFIFI010000355 DBFIFI010000000
VERSION     DBFIFI010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.357"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            320..526
                     /locus_tag="N7B39_004825"
     CDS             320..526
                     /locus_tag="N7B39_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HGU2424631.1"
                     /translation="MEKITTGVSYTTSAVGTEYWLLQLLDKVSPSQWVAIGVLGSLLF
                     GLLTYLTNLYFKIREDRRKVARGE"
     gene            complement(782..1054)
                     /locus_tag="N7B39_004826"
     CDS             complement(782..1054)
                     /locus_tag="N7B39_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424632.1"
                     /translation="MTENVKSELLLLMADNNEATSSILADPYGKISHKTLDIITTTLT
                     PLMLQRLKHNINAWVNEELSPPCLWDSRYACQQKMRIFNLLSPKLR"
     gene            1214..1747
                     /locus_tag="N7B39_004827"
     CDS             1214..1747
                     /locus_tag="N7B39_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGU2424633.1"
                     /translation="MNEKIKYGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWDIDR"
     gene            1968..2081
                     /locus_tag="N7B39_004828"
     CDS             1968..2081
                     /locus_tag="N7B39_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424634.1"
                     /translation="MKGIEVETPASLDLTRAAAFAIRIVAIAVLVWAIRWW"
     gene            2083..2343
                     /locus_tag="N7B39_004829"
                     /pseudo
     CDS             2083..2343
                     /locus_tag="N7B39_004829"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system i-spanin subunit Rz"
     gene            2303..>2406
                     /locus_tag="N7B39_004830"
     CDS             2303..>2406
                     /locus_tag="N7B39_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424635.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPA"
BASE COUNT          619 a          479 c          661 g          647 t
ORIGIN      
        1 gttacagatg ctttattaag gaaaaaaaca gccagcactg actttcggtg gagaggtgct
       61 ggctcagaag gatagttgga tttcacatga tacttatgcc tggcggtata ttttctgaca
      121 gacagtgacg ggtgttgtca agatattgtg tcatttataa cctgaatcag gggaggccgg
      181 aatgttatct ggcattttta gcagagcctg aatgccataa tcacggctcc cggagttggc
      241 cgtcagtggg tgacactggc ggcttttttg tttttcttta ctttcatttt ctgtcggcgg
      301 tgacggagac atacatcaga tggaaaaaat cacaacaggt gtgtcataca ccacgtcagc
      361 ggtggggacg gaatactggt tactgcagct gctggacaaa gtctctccgt cccagtgggt
      421 ggcaataggt gtgctgggaa gtctgctgtt tggcctgctg acgtatctga ctaacctgta
      481 tttcaaaatc agagaggacc gtcgtaaggt ggcgcgggga gagtagtcga tgaataaaca
      541 atacgaactg gttgtaaaat gaatatttct aactgaaaaa acgttccatg aggtaagaaa
      601 aggtcacagg caatcaataa caggacgtga tgaaagaccc ttgcatttgt gcgctttctc
      661 tttagatagc agcagatact gaaaatctga gttgtcgggg agtcagggat acagctgtgc
      721 aagagttggt cattgtgatt ccattgaaat cctgtatgcc atgaagggca ggattttatg
      781 gctacctgag ctttggtgat agtaagttga aaattcgcat tttttgctga catgcgtaac
      841 gagaatccca taagcaggga ggacttaatt cttcattaac ccatgcgttg atattatgtt
      901 tcagccgttg aagcatcagc ggtgttaatg ttgtggtaat aatatccagc gttttatgtg
      961 agatcttacc gtaagggtct gcaagaatgc tgcttgttgc ttcgttatta tctgccatca
     1021 gaagaagtaa ctctgattta acgttttctg tcattagttg taaaaatctt ctgcgcaaac
     1081 tttctttact gttcatttat atggcttcat ttgttgtaat ctgctgcgtc tcaagggata
     1141 tgtttatgag agcgaccatg agtgttggat tatataccta acatatcaag ggattagaaa
     1201 tcgataaatc cccatgaacg aaaaaataaa atacggcctg tcggctgccg ttctggcgct
     1261 gattggtgca ggtgcttctg cgcctgaaat cctcgaccag tttctggatg aaaaggaagg
     1321 taaccacacc acggcatacc gtgatggtgc ggggatctgg accatctgcc gtggtgccat
     1381 tctggtggat ggtaagcctg ttattcctgg catgaagctg tcaaaggaaa aatgcgaccg
     1441 ggttaatgcc atcgaacgtg acaaggcgct ggcatgggtg gagaaaaaca tccgggtgcc
     1501 gctgaccgaa ccccagaaag cggggatcgc gtcattctgt ccgtacaaca ttggccccgg
     1561 taagtgcttc ccgtcgacgt tttataaacg aattaatgca ggcgatcgaa aaggtgcctg
     1621 tgaggcgatt cgctggtgga ttaaggacgg tggcagagac tgccgtatcc gttcaaataa
     1681 ttgctacggt caggtctcac ggcgtgacca ggagagcgcg ctggcgtgct gggacatcga
     1741 cagatagcag aatattttcc tgaaaaatga cgttggccaa cgcgggtgga taacacgaaa
     1801 tcctgaaaac tggtaaaacc taagtgaata aaagtaaaaa ccccgtttgt tggcagcaag
     1861 cggggttttg tgttttctga ccttgagtaa ggcaagggag aaattatggg tagggaggta
     1921 ctttccctgt gaggaagtat aaaagattct ttctgaggtt gtccattatg aaaggcattg
     1981 aagtggagac gccagccagt ctggatttaa caagagcggc agcttttgcc attcgtattg
     2041 tggccattgc tgttctggtc tgggcaatcc gttggtggtg atatgaaccg tgttctgtgt
     2101 gtggttatca ttgtcctgct ggtggcctgt ggtgcgctta gtctggggct gaatcattac
     2161 cgtgatcacg ccatcatcta caaagagcag cgcgataaaa aagccagtga gctggagctg
     2221 gcgaacgcga caattactga tatgcagata cgccagcgtg atgtcgctgc acttgatgcc
     2281 agatactcga gggaattagc cgatgcgaga gctgaaaatg aaactctgcg tgctgatgtt
     2341 gccgctggtc gtaagcgcct gcggatcaac gccacctgct ccggtaccgt gcgtgaagcc
     2401 accggc
//
LOCUS       DBFIFI010000356         2333 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.358, whole genome shotgun sequence.
ACCESSION   DBFIFI010000356 DBFIFI010000000
VERSION     DBFIFI010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.358"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..422
                     /locus_tag="N7B39_004831"
     CDS             <1..422
                     /locus_tag="N7B39_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU2424636.1"
                     /translation="SRGFSTKIQEHLAMGVLLSLGVNHVNHLLLSYSKQSLISRCYNL
                     LLSEPGTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNALSAIQTTVKP
                     ISEIARENGYKCPSRFTERFHNRFNITPREIRKASRE"
     gene            539..640
                     /locus_tag="N7B39_004832"
     CDS             539..640
                     /locus_tag="N7B39_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424637.1"
                     /translation="MRTYNPNSLLPSQMQKCTCNSLHLAFDLCGGEA"
     gene            637..1092
                     /gene="ybcN"
                     /locus_tag="N7B39_004833"
     CDS             637..1092
                     /gene="ybcN"
                     /locus_tag="N7B39_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein YbcN"
                     /protein_id="HGU2424638.1"
                     /translation="MNLSQDGIKLHRGNFTAIGRQIQPYLEEGKCFRMVLKPWREKRS
                     LSQNALSHMWYSEISEYLISRGKTFATPAWVKDALKHTYLGYETKDLVDVVTGDITTI
                     QSLRHTSDLDTGEMYVFLCKVEAWAMNIGCHLTIPQSCEFQQLRDKQEA"
     gene            1092..1262
                     /locus_tag="N7B39_004834"
     CDS             1092..1262
                     /locus_tag="N7B39_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HGU2424639.1"
                     /translation="MATPLIRVMNGHIYRVSNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            1255..1545
                     /locus_tag="N7B39_004835"
     CDS             1255..1545
                     /locus_tag="N7B39_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HGU2424640.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNSETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTHFVDAAYAKECALEGMARTQVIWLKEGVIKA"
     gene            1542..1904
                     /gene="rusA"
                     /locus_tag="N7B39_004836"
     CDS             1542..1904
                     /gene="rusA"
                     /locus_tag="N7B39_004836"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HGU2424641.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHVSAEGQAYRDNVARIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            1901..2041
                     /locus_tag="N7B39_004837"
     CDS             1901..2041
                     /locus_tag="N7B39_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HGU2424642.1"
                     /translation="MMFEFNMAELLRHRWGRLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            2127..>2333
                     /locus_tag="N7B39_004838"
     CDS             2127..>2333
                     /locus_tag="N7B39_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HGU2424643.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLIPEKVKSRPQ
                     CCDDDAMVICGCIARLYRNNRDLHD"
BASE COUNT          630 a          531 c          579 g          593 t
ORIGIN      
        1 tttcccgagg cttctcaact aaaattcagg agcacttagc aatgggcgtt ctactttcgt
       61 taggagtgaa tcatgttaat catttacttt tatcatatag taaacaatca ttgataagtc
      121 gttgttataa cctgctgcta tccgaacccg gcacaaaatg gacagcaaac aaggttgctc
      181 gatatctcta catttctgtt tctacattac atcgccgtct agcaagcgag ggggtaagtt
      241 tccaaagtat actggacgat gtgaggttaa ataatgcgtt gtctgctata caaacgacgg
      301 taaaacctat aagcgagatt gccagagaaa atggttataa gtgtccttcg cgttttactg
      361 aaagatttca taatcgtttt aatataacac caagagagat aagaaaagct tccagagagt
      421 aaaagtgttt taagaaggag caattctatc gattttgatt ttgggaaatc aacacggcat
      481 aattatgtca ccggagcctg aacaactccg gtgacttctg cgctaaacgg ggacgtttat
      541 gcgcacatac aatccaaact ctcttctccc ttcacagatg cagaaatgca cctgcaattc
      601 tttgcatcta gcgtttgacc tctgcggagg tgaagcgtga acctctcaca agacggcatc
      661 aaattacatc gcggcaactt caccgctatc ggtcggcaga tccagcctta tctggaggag
      721 ggcaaatgct ttcgcatggt gcttaaaccg tggcgtgaga aacgcagtct ttcccagaat
      781 gcactcagcc acatgtggta cagcgaaatc agtgaatacc tcatcagcag gggtaaaacg
      841 ttcgccactc cagcttgggt aaaagatgct ctcaaacaca catatctcgg ttatgaaacc
      901 aaagacctgg ttgatgtcgt aaccggtgat atcaccacta tccagtcgtt acgccatacc
      961 tccgatcttg ataccggaga gatgtatgtc ttcctgtgta aggttgaagc ctgggcgatg
     1021 aatattggtt gccatctgac tattccgcag agctgcgagt tccagcagct ccgtgacaag
     1081 caggaggcgt aatggctaca ccgcttattc gtgtcatgaa cggacacatc tacagagtat
     1141 caaatcgtcg taagcgtaag cctgagctga agccatccga aataccaaca ctgctcggat
     1201 ataccgctag cctggttgat aaaaaatggt tgcgactggc agcaaggagg aatcatggct
     1261 gatttgagaa aagcagcgcg tggtcgggaa tgccaggtaa gaatccctgg cgtatgtaat
     1321 ggcaattctg aaacgtctgt actggcacat atccggctgg ctggattgtg cggtaccggt
     1381 atcaaaccgc cagacctgat tgccaccatt gcatgttctg cctgccacga cgaaatcgac
     1441 cgccgcacac attttgtcga tgctgcatat gcaaaagaat gcgcgctgga aggtatggcg
     1501 agaacacagg ttatctggct gaaagagggg gttattaagg cgtgaatacc tacagtatca
     1561 cattaccctg gcctccgagc aataatcgct attaccgcca taatcgcggg cgcacgcacg
     1621 tcagtgcaga ggggcaggca taccgcgata acgtcgcccg aatcattaaa aacgcaatgc
     1681 tggatatcgg cctggctatg cctgtgaaaa tccgcattga gtgccacatg ccggatcgcc
     1741 gtcgccgtga cctggataat ctgcaaaaag ccgcttttga cgcactcact aaagcaggtt
     1801 tctggctgga tgatgctcag gtcgttgatt accgcgttgt gaagatgcct gttaccaaag
     1861 gtgggaggct ggaactgacc atcaccgaaa tggggaatga atgatgtttg agtttaatat
     1921 ggcagaactt cttcgccacc gctgggggcg tctgcgctta tatcgtttcc ccggttctgt
     1981 tttgaccgat taccgaatac tgaagaatta cgccaaaacc ctgacaggag caggagtatg
     2041 aagtcagaga taacaatcaa ctaatactgt tttgttgatt tttgcttgta attggcgttc
     2101 tggtctgatt tttgtggagt aagttgatgc gtgatattca gatggttctt gagcgttggg
     2161 gagcgtgggc ggctaataat catgaagatg tgacctggtc gtccattgcc gccggtttta
     2221 agggactgat ccccgaaaaa gtaaaatcac gcccgcagtg ctgtgacgat gatgcgatgg
     2281 tgatatgcgg gtgtatagcc cgcctttacc ggaacaatcg cgatctgcat gac
//
LOCUS       DBFIFI010000357         2328 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.359, whole genome shotgun sequence.
ACCESSION   DBFIFI010000357 DBFIFI010000000
VERSION     DBFIFI010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.359"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            29..517
                     /gene="eco"
                     /locus_tag="N7B39_004839"
     CDS             29..517
                     /gene="eco"
                     /locus_tag="N7B39_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HGU2424644.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDTGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
     gene            complement(667..2313)
                     /gene="mqo"
                     /locus_tag="N7B39_004840"
     CDS             complement(667..2313)
                     /gene="mqo"
                     /locus_tag="N7B39_004840"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HGU2424645.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLNLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
BASE COUNT          543 a          650 c          585 g          550 t
ORIGIN      
        1 acctataaat gaagaaggaa agcaaaaaat gaagaccatt ctacctgcag tattgtttgc
       61 cgctttcgcc accacttccg cctgggcggc agagagcgtc cagccactgg aaaaaatcgc
      121 gccttatcca caagctgaaa aaggcatgaa gcgtcaggtg attcagttaa ccccgcaaga
      181 agatgaatct accctgaaag tagaactgtt aatcggtcag acgctggaag tcgattgcaa
      241 tttgcatcgt ctcggcggga agctggaaag caaaacgctg gaaggctggg gctatgatta
      301 ttatgtcttt gataaagtta gctcgccggt ttccacgatg atggcctgcc cggatggcaa
      361 gaaagagaag aaatttgtca ccgcgtatct gggcgatact ggaatgctgc gttacaacag
      421 caagctgccg atcgtggtgt atacgccaga caatgtagat gtgaagtacc gcgtctggaa
      481 ggcggaagag aaaattgaca acgcggtagt tcgctaaact gccgtgaagt gcggcacccc
      541 gtaggttgga taaagcggtc acgccgcatc cgacatctaa cgcccgagcc ggttgcctga
      601 tgcgacgctg gcgcgtctta tcagcatacg ccacatccgg cataccatgc cggatgtggc
      661 acatcattac aacgcaatat ccgccacttc tttttgaacg ggttgcggtt tcaactgcgg
      721 tttcggcgta ctatctgctg cttgcggttt gtcgtatttc agccccagca cttcgctggt
      781 gtactgcaac tcgcgttctg ttgccgctac atcaccgttc agcttgcgtc cataagatgg
      841 aacgatcgct ttcaacgtag cctgccattg cgggctggaa acgcgatcgc caaatacttt
      901 ttccagcaga ttcaacataa tcggcgcggc ggttgacgcc cctggcgatg cccccaggag
      961 cgcggcaatg gttccttgct ggtcactgac cacttcagta cccagacgca gtacaccacc
     1021 tttatcggca tcacgcttga taatctgcac acgctgcccc gcttgccaca aacgccagtc
     1081 ctcttttttc gcttgcggat agtactcttt caacgcttca aaacgatcct cttcactcaa
     1141 catcacctga ctcaccagat atttcaccag atcgaaatta tccagcccga cgtgcatcat
     1201 cggcatcacg ttagaggtgg tggtggaact catcagatcc cacaatgaac cgtttttgag
     1261 gaatttggtt gagaaggtgg caaatggccc aaacagcact acgcgtttac cgtccagaac
     1321 gcgggtatcg atatgcggaa ccgacatcgg tggtgcgcca acggatgctt taccgtaaac
     1381 cttcgccaga tggtgattaa ccacgtccgg gttttccgaa acaaggaact gtccgcccac
     1441 cgggaaaccg gcgtagtctt tcgcttccgg aatccccgat tcctgtaaca gcttcagtgc
     1501 cgcaccgccc gcgccgataa agacaaattt cgcacggatg ttctgtgcag tgccattttt
     1561 cagatcggca acggtaacgg tccaggtgtt atcgtcatta cgctttaggg cgcggacttc
     1621 gctgctgagt tgcagcgaga agttagattt cttctgcaag gaagcaatta actggcgggt
     1681 gatctcgccg tagttcacat cggtaccaat ttccgtacgc gtggctgcca ctttctgttg
     1741 cggatcgcgc ccttccatca ctaacggtgc ccactctttg atctgcgcgt gatcttcaga
     1801 gtaacgcata ccgcgaaaca gcgagctttg ttgcaacgcg gcgtaacggg cgcgcaggaa
     1861 attgacgtta tcctcgcccc agacaaagct catatgcgga acggtattga taaatgaacg
     1921 cggagtacgc agcacgccgc gctcgacctg gtgcgcccag aactggcggg aaatctgaaa
     1981 tgcttcgtta atggcgactg ctttttcaat actgatgctg ccatcggcgt tttgcggggt
     2041 gtagttcagt tccatcagtg cagaatgccc ggttccggcg ttattccagc cgttcgaact
     2101 ctcctgcgcg acaccctcca ggcgctccac catggtcatc gaccattcag gctccagctc
     2161 gcgtaaatag gtccccaacg tggcgctcat aatgccgccg ccaatcaaca gtacatcagt
     2221 ttcctgctcc tcggacgctt tcgcttttgc cgccatcgaa acggcattaa gccccacggc
     2281 catcgagaag agcatggcag tcactttttt catcttgtta atgcctta
//
LOCUS       DBFIFI010000358         2281 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.360, whole genome shotgun sequence.
ACCESSION   DBFIFI010000358 DBFIFI010000000
VERSION     DBFIFI010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.360"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            38..2071
                     /gene="betT"
                     /locus_tag="N7B39_004841"
     CDS             38..2071
                     /gene="betT"
                     /locus_tag="N7B39_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HGU2424646.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
BASE COUNT          461 a          555 c          646 g          619 t
ORIGIN      
        1 tgattttaaa tggtaacaat aacagtgggg atactggatg acagaccttt cacacagcag
       61 ggaaaaggac aaaatcaatc cggtggtgtt ttacacctcc gccggactga ttttgttgtt
      121 ttccctgaca acgatcctgt ttcgcgactt ctcggccctg tggattggcc gcacgctgga
      181 ctgggtttct aaaaccttcg gttggtacta tctgctggcg gcaacgctct atattgtctt
      241 tgtggtctgt atcgcttgtt cgcgttttgg ttcggtgaag ctcgggccag aacaatccaa
      301 accggaattc agcctgctga gttgggcggc gatgctgttt gctgccggga tcggtatcga
      361 tctgatgttc ttttccgtag ccgaaccggt aacgcagtat atgcagccgc cggaaggcgc
      421 gggacagacg attgaggccg cgcgtcaggc gatggtctgg acgctgtttc actacggctt
      481 aaccggctgg tcgatgtatg cgctgatggg catggcgctc ggatacttta gctatcgtta
      541 taatttgccg ctcaccatcc gctcggcgct gtacccgatc ttcggtaaac ggattaacgg
      601 gccgataggt cactcagtgg atattgcagc ggtgatcggc actatcttcg gtattgccac
      661 tacgctcggt atcggtgtgg tgcagcttaa ctatggcttg agcgtactgt ttgatattcc
      721 cgattcgatg gcggcaaaag cggcactgat cgccttgtcg gtgataatcg ccactatctc
      781 ggtgacatcc ggtgtcgata agggcattcg cgtgttatcg gagcttaatg tcgcgctggc
      841 gctgggattg atcctgttcg tgttgtttat gggcgacact tcgttcctgc ttaatgcact
      901 ggtgctgaat gttggcgact atgtgaatcg ctttatgggc atgacgctca acagttttgc
      961 cttcgaccgc ccggttgagt ggatgaataa ctggacgctc ttcttctggg catggtgggt
     1021 ggcatggtcg ccgtttgtcg gcttgttcct ggcgcgtatc tcgcgtgggc gtaccattcg
     1081 ccagttcgtg ctgggcacgt tgattattcc gtttaccttc acgctgttat ggctctcggt
     1141 gttcggcaat agcgcgctgt atgaaatcat ccacggcggc gcggcatttg ccgaggaagc
     1201 gatggtccat ccggagcgcg gcttctacag cctgctggcg cagtatccgg cgtttacctt
     1261 tagcgcctcc gtcgccacca ttactggcct gctgttttat gtgacctcgg cggactccgg
     1321 ggcgctggtg ctggggaatt tcacctcgca gcttaaagat atcaacagcg acgcccccgg
     1381 ctggctgcgc gtcttctggt cggtggcgat tggcctgctg acgctcggca tgctgatgac
     1441 caacgggata tccgcgctgc aaaacaccac ggtgattatg gggctgccgt tcagctttgt
     1501 gatcttcttc gtgatggcgg ggttgtataa atctctgaag gtagaagatt accgccgtga
     1561 aagtgccaac cgcgataccg caccgcgacc gctggggctt caggatcgcc tgagctggaa
     1621 aaaacgtctc tcgcgcctga tgaattatcc gggcacgcgt tacactaaac agatgatgga
     1681 gacggtctgt tacccggcaa tggaagaagt ggcgcaggag ttgcggttgc gcggcgcgta
     1741 cgtggagcta aaaagcctgc cgccggaaga gggacaacag ttggggcatc tggatttgtt
     1801 ggtgcatatg ggcgaagagc aaaactttgt ctatcagatt tggccgcagc aatactcggt
     1861 gccgggcttt acctaccgcg cacgtagcgg gaaatcgacc tactaccggc tggaaacctt
     1921 cctgttagaa ggcagccagg gcaacgacct gatggactac agcaaagagc aggtgatcac
     1981 cgatattctt gaccagtacg agcggcacct taactttatt catctccatc gtgaagcgcc
     2041 gggccatagc gtgatgttcc cggacgcgtg attgttattg cataaaaccg cgccatgtct
     2101 gcatatggcg cgggaatcat tgactcgctg taaaaaatta tgcatatatt atatatgatt
     2161 gtcatgtcat atcctgaaat tcctcttgta tcacttcatg attttatatt ttattaatta
     2221 acgattttat tttgtattag ctgaatggat tcagtcctat agggttatat atttattaat
     2281 a
//
LOCUS       DBFIFI010000359         2255 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.361, whole genome shotgun sequence.
ACCESSION   DBFIFI010000359 DBFIFI010000000
VERSION     DBFIFI010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.361"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..1217
                     /locus_tag="N7B39_004842"
     CDS             <1..1217
                     /locus_tag="N7B39_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HGU2424647.1"
                     /translation="TFDNGAPDLNIKPGQKVVFDVDVEHGPLPITMLMHGNVIPALAA
                     AKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAPAEYGKFSFGEGQFT
                     FNGDSSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLEEKKFPVGESESKFN
                     QINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLTKGNQQLGSGEWSLI
                     AESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAALFKEYLPLLQQSEPT
                     IKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVKLPLNVVTETAKQLN
                     LSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRYTPGKVVFNGQEMSE
                     EEFMSRAGRFVH"
     gene            complement(1258..2190)
                     /gene="yihG"
                     /locus_tag="N7B39_004843"
     CDS             complement(1258..2190)
                     /gene="yihG"
                     /locus_tag="N7B39_004843"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HGU2424648.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
BASE COUNT          697 a          488 c          502 g          568 t
ORIGIN      
        1 tgacattcga taacggtgca cccgatctca atatcaagcc aggccagaaa gttgtatttg
       61 atgtggatgt tgagcacggt ccgttgccca tcacaatgtt aatgcatggt aatgtcatcc
      121 cagcactggc agcggcaaaa gtgaacttag tgaataatga actgacacaa ccgctattta
      181 tcgccgcgaa aaataaatcg cccgtggaag cgacattgcg attcgcgttt ggtggctcat
      241 tctctacgac attagatgtt gcccctgcag agtatggaaa gttttctttt ggtgagggcc
      301 agtttacttt taatggtgat agtagttcat tgtctaacct ggatattgaa ggcaaagtcg
      361 aagatattgt tctgcaatta tcaccaatga acaaagtaac ggcaaaaagt tttaccattg
      421 attctctggc gcgattagaa gaaaagaaat ttccggttgg tgaaagcgag tcgaaattta
      481 atcagattaa cattatcaat cacggggaag acgttgccca aatcgatgct ttcgttgcaa
      541 aaaccaggct ggatcgcgtt aaagacaaag attatatcaa tgtcaatctg acctacgaac
      601 ttgataagtt aacaaaaggg aatcagcaac tcggtagtgg tgagtggtca ttgattgctg
      661 aatctattga tccctcagcg gtgcgccaat ttatcatcca gtataacatt gcgatgcaga
      721 agcagcttgc tgcacaccct gaattagcaa acgatgaagt tgctctgcaa gaagtgaatg
      781 ctgcattgtt caaagagtat ttaccgttat tacaacaaag tgagccgacc attaaacaac
      841 cggtaagatg gaagaacgca ctcggcgaac taaatgccaa tctggatatc agtattgccg
      901 acccagccaa atcttcatca tccacaaaca aagatatcaa atcgctcaat tttgatgtga
      961 agttaccgct taatgtcgtc acagaaaccg caaaacagct taatttatct gaaggaatgg
     1021 atgcggaaaa agcgcaaaag caggctgata aacaaatcag cgggatgatg acattaggtc
     1081 agatgtttca gttaatcacg attgacaaca ataccgcctc gctgcagctg cgttatacac
     1141 cgggtaaagt tgtttttaac ggacaggaga tgagcgaaga agaatttatg tctcgtgccg
     1201 gacgttttgt tcattaaaat actcactcgc ctctgctcat tcagaggcga gcaaaattta
     1261 cttgtcctgc cgctgtgatg acatcaacga cgtcagaagt cggtcttttt cctgccacaa
     1321 tgagttcagc cattgctgaa aatggcgctt aaagcttttg tcattgatgt agtcgccatg
     1381 taactcatcg gcaataggct gtaaatccac atgtacgaca atccgcgtca gtttaccgct
     1441 taacatatcg aagaacggct gacggttatt gtccggataa cacaacgtaa cattaagcag
     1501 tttatcgaat tgtttaccca gtacatttag cgccatcgca atgcctgccg cctttggtgg
     1561 caacaagttt tggaaagtgg agtgggtttg ctgatgtttt tcttgcgtga aacgggagcc
     1621 ttcaacgaaa ttgacaatgg tggtgggatg caggcgaaac ttttcacaag aacgccgagt
     1681 ggtttcaaca tctttaccac gtcgctccgg atgacgtaat aaataagcgc gggaataacg
     1741 cttcataaac ggcatatcca gcgcccagca cgccaggcca agaaatggca cccaggccag
     1801 ctgttgcttg aggaaatatt tattcatcgg aatgtgctta cgaaacagca cgcacagtac
     1861 gacaatatct gcccaactac ggtggttaca aataagcaga taccagttct tcttacttaa
     1921 cccttccagc ccgtgaactt cccattgcag gtgtgggttc agatgcagta ataccgccag
     1981 accttcacac cagcaataca tcataaaatc acaaaaacgc gaaacctttc gccaaatgac
     2041 tggcacaggt agcaatagtt ttacaatccc ggcaatgatg atcgggacgg aacaaaaaat
     2101 agtgaccaaa atggtcaata cgatactcag caaaagggtt atcgcagcga gtattctcgt
     2161 cataatgaat ttattcaaaa ggttagccat agacagtgcg ctaaagaagc gcaaggcgca
     2221 gattttagca gaaaacgacc caaataacgg atgat
//
LOCUS       DBFIFI010000360         2238 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.362, whole genome shotgun sequence.
ACCESSION   DBFIFI010000360 DBFIFI010000000
VERSION     DBFIFI010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.362"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            345..1322
                     /locus_tag="N7B39_004844"
                     /pseudo
     CDS             345..1322
                     /locus_tag="N7B39_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041206064.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            1842..>2238
                     /locus_tag="N7B39_004845"
     CDS             1842..>2238
                     /locus_tag="N7B39_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424649.1"
                     /translation="MRLPEKVLFPPVTSGLSGQEKQKKPKSITGFQENYQRNIRPIKT
                     ASEARLRFFDKMVSKENSLEDVVSLGEMIQKEIYGHEQRTFSPVHHTGNWKSSLLHNA
                     LLGLANVYNGLRETEYPNTFNRDGIKSTNS"
BASE COUNT          696 a          479 c          487 g          576 t
ORIGIN      
        1 ttaattctat ttcattatat gagaacacca ataagaataa taatatttga tcacaactga
       61 agatgctttt tacaatgaag aattattgtt aaaaaacgct ttgttgaatc aaccgaactg
      121 ttgctgagtt gcaggatcac atcacgtatt ttcctgaaaa cgcaggctgc ttagtagcaa
      181 agcagaagtt caaaatcacc aactaaatag ctgcgccgaa tagtagatca atgaaaggga
      241 actcagcccg gattgtgcga tctgatcaat cgccaaaaat ccccccctaa ccggactgag
      301 cgatgccgat catagcacca attccccgcg acgaatgacg cctgatgcaa aaagcaatct
      361 ataaaactca cgataaaaat tatgcccgca ggctgaccgc catgctgatg ctgcaccgga
      421 gcgctcgcgt cagtgacgtt gccagaacgc tctgctgcgc ccgttcctcc gtcaggcgct
      481 ggattaactg gttcacgctg tcgagtgttg aaggtctgaa atcattaccc gcaggacgtt
      541 cccgccgctg gccgtttgag catatctgca cattgtttcg tgaactgata aaacactctc
      601 ccggatattt tggctatcag cgttcacgct ggagtacaga actgctggca ataaaaatca
      661 acgagataac cgagtgccag ttacatgccg gaaccgttcg ccgctggttg ccattagtcg
      721 ggcttgtgtg gcgcagggct gcgccaaccc tgcgaacccg taacccgcat aaagcagcaa
      781 ttcacaaagc actggacgaa tgtagtgcgg agcatccggt cttttatgaa gatgaagtgt
      841 atatctatct taatcccaaa accggtgcgg actggcaact tcgcagacag caaaaacatg
      901 tggtaacacc ggggcagaat gaaaaatact atctggctgg tgctttacat agtggaacgg
      961 gcaaagtcag ctatgtgggt ggcaacagca aaagctcagc cttatttatc agtctgttga
     1021 aacatctgaa attgacatac cgacgggata aacctatcac gctcatagtg gacaactaca
     1081 tcatccataa aagtcgggaa acacagcgct ggctgaaaga aaatccgaag ttcagagtga
     1141 tttatcagcc agtttactcg ccgtgggtga atcatgttga gcggttatag caggcgcttc
     1201 acgatacgat aacgcgcaat catcagtgcc gttcaatgtg gtaactgctt aaaaatgttc
     1261 attattttat ggaaaccgtg agcccattcc ccggtggtaa atatgggctg ttaaaagtgt
     1321 agcggtattc ggcgcagcta tttaggatgg tcaggtcgca gaagttctgt gccactacag
     1381 tgcgtgtact aaacaaaatg acgagggcac gaatgtcata atacgtgcgt attacctgac
     1441 agtagctacc aactgagggg gcttattcca aacctgattt attcaacaaa gctggactta
     1501 tccttactct gaattatttt ccagacatat gcatcatgta ctggctcgct tggttcagga
     1561 aatacaggag caaatctaca gctattagat ttattcctat ttgtatgcaa agtcgcattg
     1621 cataatgcgg agctatgtta cagatacgcc tgacgtgcgt agtaaaatta taaagcaaca
     1681 tatatataca aggagaaacc gagaaaaata aatgagtcgt atgttgggta gcatcgagat
     1741 aaatattttt tttatcacat tagcaccttg gttaagggtg ttaataataa tattctctca
     1801 cttattaaaa cataaataat tttttaagcg gaaatgtaat tatgagactg ccagagaaag
     1861 ttctttttcc tcctgtcact agtggcctgt cagggcagga aaaacaaaaa aaaccgaaga
     1921 gcattaccgg atttcaggaa aattatcaac gcaatatcag gccaatcaaa acagcatcag
     1981 aagcccgact acgcttcttt gataaaatgg tttcgaaaga aaactctctg gaagatgttg
     2041 tttctttagg tgaaatgatt cagaaggaaa tttatgggca tgaacaaaga acattttcac
     2101 cagttcatca tacaggtaac tggaaatcat cattgttaca caacgcgctc cttggtctgg
     2161 caaatgttta taatggctta cgggaaacag aataccctaa cactttcaac agagatggta
     2221 taaaaagtac taactctt
//
LOCUS       DBFIFI010000361         2212 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.363, whole genome shotgun sequence.
ACCESSION   DBFIFI010000361 DBFIFI010000000
VERSION     DBFIFI010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.363"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..1148
                     /gene="escC"
                     /locus_tag="N7B39_004846"
     CDS             <1..1148
                     /gene="escC"
                     /locus_tag="N7B39_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE outer membrane
                     ring protein EscC"
                     /protein_id="HGU2424650.1"
                     /translation="NKNSCNVRKITTFNSIEVRGVPECIKYITSLSESLDKEAQSKAK
                     NKDVVKVFKLNYASATDITYKYRDQNVVVPGVVSILKTMASNGSLPSTGKGAVERSGN
                     LFDNSVTISADPRLNAVVVKDREITMDIYQQLISELDIEQRQIEISVSIIDVDANDLQ
                     QLGVNWSGTLNAGQGTIAFNSSTAQANISSSVISNASNFMIRVNALQQNSKAKILSQP
                     SIITLNNMQAILDKNVTFYTKVSGEKVASLESITSGTLLRVTPRILDDSSNSLTGKRR
                     ERVRLLLDIQDGNQSTNQSNAQDASSTLPEVQNSEMTTEATLSAGESLLLGGFIQDKE
                     SSSKDGIPLLSDIPVIGSLFSSTVKQKHSVVRLFLIKATPIKSASSE"
     gene            1162..1617
                     /gene="cesD"
                     /locus_tag="N7B39_004847"
     CDS             1162..1617
                     /gene="cesD"
                     /locus_tag="N7B39_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone CesD"
                     /protein_id="HGU2424651.1"
                     /translation="MSRKFSSLEDIYDFYQDGGTLASLTNLTQQDLNDLHSYAYTAYQ
                     SGDVITARNLFHLLTYLEHWNYDYTLSLGLCHQRLSNHEDAQLCFARCATLVMQDPRA
                     SYYSGISYLLVGNKKMAKKAFKACLMWCNEKEKYTTYKENIKKLLGNTE"
     gene            complement(2001..>2212)
                     /gene="grlA"
                     /locus_tag="N7B39_004848"
     CDS             complement(2001..>2212)
                     /gene="grlA"
                     /locus_tag="N7B39_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE transcriptional
                     regulator GrlA"
                     /protein_id="HGU2424652.1"
                     /translation="ISFRVRYVSYGNLHYKRLEIFIYDVNLEAVPIESPGTTGPKRKT
                     YRVGNGIVGQSNIWNEMIMRRKKES"
BASE COUNT          732 a          426 c          406 g          648 t
ORIGIN      
        1 taaacaaaaa ctcttgcaat gtgagaaaaa ttacgacatt taactcgatt gaagttcggg
       61 gtgttcctga atgtataaaa tatattacat ctttatcaga aagtcttgat aaagaggcac
      121 agtctaaagc caaaaataaa gatgttgtta aggtctttaa actgaactat gcgtcagcga
      181 cagatataac atacaaatac cgtgaccaaa atgttgtcgt cccaggtgtt gtctccattc
      241 tgaagacaat ggcaagtaat ggttcacttc ccagtaccgg taaaggtgcc gtcgagcgaa
      301 gcggtaacct gtttgataac agtgtaacga tttccgctga tccacgtctt aatgcagttg
      361 ttgtaaaaga ccgcgaaata acgatggata tctatcagca attaatttct gagcttgata
      421 ttgaacaaag gcaaattgaa atttctgtct caattattga tgttgatgca aacgatcttc
      481 agcagctcgg cgtaaactgg tccggtacgt taaatgcagg acaaggtacg attgccttta
      541 atagctctac cgcacaggct aatatcagct cttctgtgat aagtaatgcc agtaatttta
      601 tgattagggt gaatgcgtta caacagaact cgaaagctaa aatactctct cagccttcaa
      661 ttataacctt aaataatatg caggcgattc ttgataaaaa cgtaacgttt tatacaaaag
      721 tctccggaga aaaggtggcg tctctggaaa gtattacaag cggaacattg ctgagagtga
      781 caccacgtat acttgatgac agctcgaata gccttaccgg aaaacgtcgg gaacgagttc
      841 gtttgttact tgatattcag gatggcaatc aatcgactaa ccaaagcaat gctcaggatg
      901 ccagcagcac cttacctgaa gtacaaaatt cagaaatgac aacagaagcg acattaagtg
      961 caggagaaag tttattactt ggcggattta tccaagataa agaaagttca agtaaagatg
     1021 gtataccgct gttaagcgac attcctgtta ttggtagttt attctccagt acggtaaaac
     1081 aaaaacatag tgttgttcga ctctttttaa ttaaagcaac cccgataaaa tctgcatcta
     1141 gcgaataata aatgaggctt tatgagcagg aaatttagct ctctagagga tatttatgat
     1201 ttctaccagg atggtggcac attagcgtca ttaacaaatc tgacacaaca agatctcaat
     1261 gaccttcatt cttatgccta tacagcatat caatctggtg atgttataac cgcaagaaat
     1321 ctattccatt tgctcacata tctggaacac tggaattatg actacacctt atctctgggc
     1381 ttatgtcatc agcgtttatc aaatcatgaa gatgcacaac tgtgtttcgc acgctgtgca
     1441 actttagtta tgcaagatcc cagggcatct tattattctg gaattagcta cttactcgtc
     1501 ggaaataaga aaatggccaa gaaagccttt aaggcttgtt taatgtggtg taatgaaaaa
     1561 gaaaaataca ctacatataa agaaaatatt aaaaaattgt taggtaatac agagtaaaaa
     1621 ctaccatcag ctataccaat ccccaggctg actggggatt ttctatcgga aataataaag
     1681 ctgctatagt gaagtgctcc caaagttgag caccgaccac tggtagattt atctacaggt
     1741 gtggggttat tccattaacc aggagtttat gccctgaact atcttctgct gtatttaata
     1801 tcatttccgt ttatgaaaca atcagtatat cttaacttag cattagaaat ctgatctttc
     1861 caatcatcta tagctcagag tatatttgtc aggaaatgac cttcaaactt tagccgagaa
     1921 aaaggcttac cctggaaaac aaaaccctta aatatagctt tatttttatt cttctataaa
     1981 atatactcaa aaaattacgt ctaactctcc tttttccgcc tcatgatcat ttcgttccag
     2041 atattagact gtcccacaat accattacca actcggtagg tttttctttt tggtccggtt
     2101 gttccaggac tttctatcgg aaccgcctca aggttaacat cataaatgaa aatctcaagg
     2161 cgcttataat gcaaattacc ataactaaca tatctgacac gaaatgaaat tt
//
LOCUS       DBFIFI010000362         2192 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.364, whole genome shotgun sequence.
ACCESSION   DBFIFI010000362 DBFIFI010000000
VERSION     DBFIFI010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.364"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            264..809
                     /locus_tag="N7B39_004849"
     CDS             264..809
                     /locus_tag="N7B39_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HGU2424653.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AAIKWYAERDAEIENEKLRREVEELRQDSETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            784..>2192
                     /locus_tag="N7B39_004850"
     CDS             784..>2192
                     /locus_tag="N7B39_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HGU2424654.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADASYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFTNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSASVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSVSRICWDTGGI"
BASE COUNT          525 a          523 c          637 g          507 t
ORIGIN      
        1 agatgcacgt tgtgacatgt agatgataat tattatcatt ttgcgggtcc tttccggcga
       61 tccgacaggt tacggggcgg cgacctcgcg gtttttcact atttatgaaa attttccggt
      121 ttaaggcgtt tccgttcttc ttcgccgtaa cctaatgttt ttatttaaaa caccccctga
      181 aaagaaagga aacgacaggt gctgaaaacg ggctttttgg cctctgtcgt ttcctttctc
      241 tgtttttgtc cgtggaatga acaatggaag tcaacaaaaa gcagctggct gacattttcg
      301 gcgcgagtat ccgtaccatt cagaactggc aggagcaggg aatgcccgtt ctgcggggtg
      361 gtggcaaggg taatgaggta ctttatgact ctgccgccgc cataaaatgg tatgccgaaa
      421 gggatgctga aattgagaac gaaaagctgc gccgggaagt tgaagaactg aggcaggaca
      481 gcgagacaga tctccagcca gggactattg agtacgaacg ccatcgactt acgcgtgcgc
      541 aggccgacgc acaggaactg aagaatgcca gagactcagc tgaagtggtg gaaaccgcat
      601 tctgtacttt cgtgctgtcg cggatcgcag gtgaaattgc cagtattctc gacgggatcc
      661 ccctgtcggt gcagcggcgt tttccggaac tggaaaaccg acatgttgat ttcctgaaac
      721 gggatatcat caaagccatg aacaaagcag ccgcgctgga tgaactgata ccggggttgc
      781 tgagtgaata tatcgaacag tcaggttaac aggctgcggc attttgtccg cgccgggctt
      841 cgctcactgt tcaggccgga gccacagacc gccgttgaat gggcggatgc cagttactat
      901 ctcccgaaag aatccgcata ccaggaaggg cgctgggaaa cactgccctt tcagcgggcc
      961 atcatgaatg cgatgggcag tgactacatc cgcgaggtga atgtggtgaa gtctgcccgt
     1021 gttggttatt ccaaaatgct gctgggtgtt tatgcctact tcatagagca taagcagcgc
     1081 aacaccctta tctggttgcc gacggatggt gatgccgaga actttatgaa aacccacgtt
     1141 gagccgacta ttcgtgatat tccgtcgctg ctggcgctgg ccccgtggta tggcaaaaag
     1201 caccgggata acacgctcac catgaagcgt ttcaccaatg ggcgtggctt ctggtgcctg
     1261 ggcggtaaag cggcaaaaaa ctaccgtgaa aagtcagtgg atgtggcggg ttatgatgaa
     1321 cttgctgcct ttgatgagga tattgaacag gaaggctctc cgacgttcct gggcgataag
     1381 cgtattgaag gctcggtctg gccaaagtcc atccgtggct ccacgcccaa agtgagaggc
     1441 acctgccaga ttgagcgtgc agccagtgaa tccccgcatt ttatgcgttt tcatgttgcc
     1501 tgcccgcact gcggggagga gcagtacctt aaatttggcg ataaagagac gccgtttggc
     1561 ctcaaatgga cgccggatga tccctccagc gtgttttatc tctgcgagca taatgcctgc
     1621 gtcatccgtc agcaggagct ggactttact gatgcccgtt atatctgcga aaagaccggg
     1681 atctggaccc gtgatggcat tctctggttt tcgtcatccg gtgaagagat tgaaccgcct
     1741 gacagtgtga cctttcacat ctggacggcg tacagcccgt tcaccacctg ggttcagatt
     1801 gtcaaagact ggatgaagac gaaaggggat acgggaaaac gtaaaacctt cgtgaacacc
     1861 acgctcggtg agacatggga agcgaagatc ggcgaacgtc cggatgctga agtgatggca
     1921 gagcggaaag agcattattc agcgtccgtt cctgaccgtg tggcttacct gaccgccggt
     1981 atcgactccc agctggatcg ctacgaaatg cgcgtatggg gatgggggcc gggtgaggaa
     2041 agctggctga ttgatcggca gattattatg ggccgccacg acgatgaaca gacgctgctg
     2101 cgtgtggatg aggccatcaa taaaacctat acccgccgga atggtgcaga aatgtcggta
     2161 tcccgtatct gctgggatac tggcgggatt ga
//
LOCUS       DBFIFI010000363         2181 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.365, whole genome shotgun sequence.
ACCESSION   DBFIFI010000363 DBFIFI010000000
VERSION     DBFIFI010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.365"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(65..991)
                     /locus_tag="N7B39_004851"
     CDS             complement(65..991)
                     /locus_tag="N7B39_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU2424655.1"
                     /translation="MMKIEPSILPSLAWFALIVRAGSFSRAASEMGITLAALSQNLKS
                     LEERLNTKLIYRTTRNISLTEEGQRLYEVLVSALGQIDDALKDVGDTQLEPTGLLRIN
                     SSRVAARMLVEPHIGEFLTRYPKTKIELIMDDGLSNIIADGCDVGIRLEQGLDEHMTA
                     VPVSPLIKLVTVASPDYLKEHGIPETPQELSNHNCLRLRHKSSGALSAWEFSKVVGGN
                     EEFEIEVSGKYISNDDESMIRMALNGTGIIQHLDFAIAEHINAGKLQPILEDWAVSFP
                     GFYIYVSSRVRMPSKVRAFIDFMVEKRVKLEN"
     gene            1148..2068
                     /locus_tag="N7B39_004852"
     CDS             1148..2068
                     /locus_tag="N7B39_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU2424656.1"
                     /translation="MKTVSIKHTYWDIAADIYFPDDFNSEKKYPAIISAHPIGSCKEQ
                     TSGSVYGAALAKAGFIVIAFDASFQGSSGGEPRYLEDPTMRVKDFSIVVDYLTSLPYV
                     DAGRIGVLGICGGGGYAINAAMTERRIKAIGTVTGANYGRLMREGFTAFNPIGALEAM
                     AQQRTDEANGAKLRVDDLLPSSPDAALEAGLTEIDLFEATEYYRSPRGYAPNGVNRSL
                     FSHQTVAVGWDAFHLAEVLLTQPIMVVVGDRVGAFGAYRDGCEIIGRAASKHKELVVV
                     EGYSHYDLYDKPEPVKQALDKLIPFYKTHL"
BASE COUNT          657 a          491 c          446 g          587 t
ORIGIN      
        1 cctaatgatt acgttattcc tcttaccgat attcgcccag agcaaccatc aattacacat
       61 atcatcaatt ttcgagtttt actcttttct ccaccataaa atcaataaaa gcacgaactt
      121 tagagggcat tctcacccgt gatgaaacat aaatataaaa cccaggaaat gaaacagccc
      181 aatcctcaag aatcggctgt aacttgcccg cattgatatg ttcagcgatt gcaaaatcca
      241 gatgttgaat gatacccgtt ccattcagcg ccattcgtat catgctttcg tcatcattag
      301 aaatatactt acctgaaacc tctatttcga actcttcatt accccccaca accttagaaa
      361 actcccatgc agagagtgca ccgctacttt tatgccgcaa ccgaaggcaa ttatggttac
      421 tcagttcctg aggcgtttca ggaataccgt gttcttttaa ataatctggc gatgccacag
      481 taaccagttt aataagcgga gaaacaggaa cggcagtcat atgctcatcc agtccttgct
      541 caagacgtat gcccacatca cagccgtccg caataatgtt agataaaccg tcatccataa
      601 tgagttctat ctttgttttg gggtaacgag taagaaactc gccaatatga ggctcaacaa
      661 gcatccgtgc tgcaacacgg gaggaattta ttctcaacag gcctgtaggt tcaagttggg
      721 tatcaccaac atctttcaaa gcatcatcaa tttgccccaa agccgacacc agcacttcat
      781 acagacgctg tccttcctca gtaagcgaga tattgcgggt cgtacggtat atcagcttgg
      841 tgttaagacg ttcctcaaga gattttaaat tctgggacaa tgcggccagc gtaataccca
      901 tttcagaagc ggctctggaa aaactgccgg cacgaacaat cagcgcaaac caggcaagag
      961 aaggcaaaat tgaaggctct attttcatca ttgtaaacct taacttaaca ctgtgataga
     1021 tattagacca ttgatgataa ccaatacaag tcatactatt acgccgttct cgatatgaac
     1081 acaaatgcca gttgcggata atagaaatga tatttaaatc tgtttaggca aatccccgga
     1141 gcttgaaatg aaaactgttt cgataaaaca cacttactgg gatatagctg ctgatatcta
     1201 tttccctgac gattttaata gtgaaaaaaa gtatcctgcc attatcagcg cccatccaat
     1261 tggaagctgc aaagaacaga catcgggtag tgtgtatggt gccgccctgg caaaagctgg
     1321 ctttatcgtc attgcttttg atgctagttt tcagggaagc agcggtgggg aaccgcgtta
     1381 tctggaagat ccaacaatgc gagtaaaaga cttcagcatt gtcgttgatt accttaccag
     1441 cttaccttac gttgatgccg ggcgtattgg tgtactgggt atttgtggtg gaggtggata
     1501 tgccattaat gccgcaatga cagaacgccg aattaaagct attggtactg tcacgggtgc
     1561 aaactacggg cgtcttatgc gtgagggatt tacagcgttt aatcctatcg gagcacttga
     1621 agcaatggcg caacaaagaa cagacgaagc taacggcgca aaacttcgcg tagatgattt
     1681 actcccctct tctccggatg cagctcttga agcgggacta acagaaatag acctttttga
     1741 agccacagaa tattatcgtt cgccacgagg ctatgcacct aatggggtaa atcgttcgct
     1801 attctcacat cagactgttg ccgtgggttg ggatgcattt catcttgcag aagtgctgtt
     1861 aactcaacca ataatggttg tggtgggcga tagagtgggc gcatttggtg cctaccgtga
     1921 cggctgcgaa attattggcc gcgctgcttc aaaacataaa gagctggtcg tggttgaagg
     1981 ttattcacat tacgacctgt atgataagcc agaacctgtg aaacaggcac tcgataaatt
     2041 aattcctttt tataaaacac atttgtaata attttacagg gagatccctt atctccctga
     2101 catttatcct ttattgacta cacaacaata ataaaattat tcaaaaagaa atttcattaa
     2161 aaaacaaaaa agcagcaact c
//
LOCUS       DBFIFI010000364         2175 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.366, whole genome shotgun sequence.
ACCESSION   DBFIFI010000364 DBFIFI010000000
VERSION     DBFIFI010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.366"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..207
                     /locus_tag="N7B39_004853"
     CDS             <1..207
                     /locus_tag="N7B39_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HGU2424657.1"
                     /translation="KAKTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVPYGEMLVGSR
                     RISQDISTRDEGGDGKVVVIGRQA"
     gene            complement(398..925)
                     /gene="sodC"
                     /locus_tag="N7B39_004854"
     CDS             complement(398..925)
                     /gene="sodC"
                     /locus_tag="N7B39_004854"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HGU2424658.1"
                     /translation="MKCKIIAAIAMLTAASCGYAAEQEVPMNLVSADGKEVSIGKITI
                     QETPYGLLFTPALHSLSEGIHGFHVHEKGNCAPALKDGKPVAALSAGGHFDPKNTGKH
                     LGPWSPDGHLGDLPALFVTHDGKANYPVLAPRLNSLKEIKGRSLMLHAGGDNHHDHPE
                     PLGGGGARMACGIIQ"
     gene            1059..>2175
                     /locus_tag="N7B39_004855"
     CDS             1059..>2175
                     /locus_tag="N7B39_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU2424659.1"
                     /translation="MGKGGGRAHTPVEAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETALGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLLETTSKGDRNPSSVRLLIQLQRNGNWVTEKDVTINGKTT
                     SQFLASVILDNLPPRPFNIRMVRETADSTSDQLQNKTLWSSYTEIIDVKQCYPNTAIV
                     GLQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYAIAQYCDQMVPDGFGGTEPRMTFNAYLSQQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDSPSDVVWPYT"
BASE COUNT          518 a          546 c          645 g          466 t
ORIGIN      
        1 aaggcaaaaa caccggatta ccgcgcaacg gataacggca gacagaacac gtacttttcc
       61 tcgctggata acatgattgc ccaggggaac ccgatgccgg tgccttacgg tgaaatgctg
      121 gttggctccc ggcgaatctc ccaggacatc agtacccgtg atgaaggcgg tgacgggaag
      181 gtggtggtta tcgggcggca ggcataaaag cgaaaaaatc ccgcagtgct cacggacagg
      241 aactgcggga gcgttacgaa gattgagtgt aaggaattat tcttatgtca cgacaaaaac
      301 attaactcag agagggagga tgtgccgttc ttttcaggga gaaaggattt atcgtcctga
      361 ggaataaagg taaggggccc gccccttacc tgactgatta ttgaatgatg ccgcaggcca
      421 ttctcgcacc accaccgccc aggggctccg gatggtcatg atggttatca ccgccagcat
      481 gaagcatgag agaacgccct ttaatctctt ttaatgagtt cagtctcggg gccaggaccg
      541 ggtagttcgc ttttccgtca tgcgtcacga acagcgcagg gaggtcgccc aggtgtccat
      601 ccggagacca ggggccaaga tgtttgccgg tgtttttcgg gtcaaagtga ccgccagccg
      661 ataatgctgc gaccggtttt ccgtctttca gtgccggggc gcaatttcct ttttcgtgca
      721 catgaaaacc atgaatgcct tcagacagag agtgaagggc tggtgtgaac agcagaccgt
      781 agggggtctc ctgaatggtt atttttccaa tgctgacttc ttttccgtca gcactgacaa
      841 ggttcattgg gacttcctgt tctgctgcgt atccgcatga tgctgctgtc agcatggcaa
      901 tggcagcaat gattttacat ttcataaaac cctcattaat tccgttaaca gactgagctt
      961 gctggttaca gggtaacaaa cagcgttctg atgatatcgc gcaatagctg tgcaatatcc
     1021 tatcactgcg attaataata ccaattgaga ggaacattat gggtaaaggt ggcggcaggg
     1081 cgcacacgcc ggttgaggca aaggacaatc ttaagtccac gcagatgatg agcgtgattg
     1141 atgccattgg tgaagggccg attgaaggtc cggtgaaggg gctgcagagt atcctggtga
     1201 acaaaacccc gctgacggac acggacggta atcctgtgat acatggtgtg acagcggtct
     1261 ggcgcgccgg ggagcaggag cagacaccac ctgaaggctt tgagtcttcc ggagctgaaa
     1321 ccgcactggg cgtggaagtg acgaaggcaa agccggtgac gcgcaccatt acatccgcga
     1381 acattgaccg cctgcgggtc accttcgggg tgcagtcact gttggagacc acctcaaagg
     1441 gcgaccgtaa tccctcttct gtccgactgc tgattcagtt gcagcgtaac ggtaactggg
     1501 tgacggaaaa ggatgtcacc attaacggca agaccacctc gcagtttctg gcgtcggtga
     1561 ttctggataa tctgcctccc cgtcctttta acatccggat ggtccgggag acggcggaca
     1621 gcacctcgga ccagctgcag aataagacgc tctggtcgtc atacaccgaa atcatcgatg
     1681 tgaaacagtg ctacccgaac acggcgattg tggggctgca ggtggatgcg gagcagtttg
     1741 gtggccagca gatgacggtg aactaccata tccgaggtcg catcatccag gtgccgtcaa
     1801 actatgaccc ggaaaaacgc acgtacagcg gcatctggga cggcagcctg aaaccggcat
     1861 acagcaacaa cccggcctgg tgcctgtggg acatgctgac tcacccgcgc tacggcatgg
     1921 gaaaacgcct gggggcggcg gatgtggaca agtgggcgct gtatgccatt gcgcagtact
     1981 gcgaccagat ggtccctgat ggtttcgggg gcacagagcc gcggatgacc tttaatgcgt
     2041 acctgtcaca acagcgtaag gcgtgggacg ttctcagtga tttctgctcg gcgatgcgct
     2101 gtatgccggt atggaacggc cagacgctga cgttcgttca ggacagcccg tcggatgtgg
     2161 tgtggccgta cacca
//
LOCUS       DBFIFI010000365         2147 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.367, whole genome shotgun sequence.
ACCESSION   DBFIFI010000365 DBFIFI010000000
VERSION     DBFIFI010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.367"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..185)
                     /gene="holE"
                     /locus_tag="N7B39_004856"
     CDS             complement(<1..185)
                     /gene="holE"
                     /locus_tag="N7B39_004856"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HGU2424660.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLA"
     gene            324..698
                     /gene="yobA"
                     /locus_tag="N7B39_004857"
     CDS             324..698
                     /gene="yobA"
                     /locus_tag="N7B39_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HGU2424661.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKRLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            702..1574
                     /gene="copD"
                     /locus_tag="N7B39_004858"
     CDS             702..1574
                     /gene="copD"
                     /locus_tag="N7B39_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HGU2424662.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLTKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWKDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGVGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLLIISLLATLEPF"
     gene            1587..1928
                     /locus_tag="N7B39_004859"
     CDS             1587..1928
                     /locus_tag="N7B39_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HGU2424663.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDVAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
BASE COUNT          524 a          523 c          538 g          562 t
ORIGIN      
        1 gccaaacggt gggcaataag ccgctcgcga aaccagctgc gcaaatgttc aggctgttca
       61 cgttcaaccg cttcagcgat cactggcata ttgtagcgtt ctttaaatgc caccccggcc
      121 gccgccaaat cgacattcac tttatccatt tctgtttgat ccagtttagc cagattcttc
      181 agcattctta atctcctcat cattcgcggg caaaagttac taagagtgag cctggatggc
      241 aagccggaat tgatgatcct cgactcagac attagacaag gttatgctct gtaaaaaagt
      301 tattacaaaa aggaatagat aatatggctt caactgcacg ctcccttcgc tacgcgctgg
      361 caatcctgac cacttcgttg gtgacacctt ccgtctgggc acatgcgcat ttaacgcatc
      421 agtatcctgc ggcaaacgcg caagtgacag ctgcaccgca ggcaatcact ttaaacttct
      481 cggaaggtgt tgaaacagga ttcagtggtg caaaaatcac ggggccaaaa aacgaaaata
      541 ttaaaacatt accggcgaag cgaaatgagc aggaccaaaa gcgactgatt gttccactgg
      601 ccgattcgct gaaacccgga acctacaccg ttgactggca tgttgtttcg gtggacgggc
      661 ataaaacgaa aggacactac acctttagcg tgaaataaat catgctggcg tttacctgga
      721 tcgcgctgcg atttatccat ttcacctcgc tgatgctggt ctttggcttc gcaatgtacg
      781 gtgcctggct ggcaccctta acgattcgtc gcttgctgac gaagcgtttt ctgcgtttac
      841 aacagcacgc tgccgtctgg agtttgatca gtgccactgc aatgctcgcc gttcagggcg
      901 ggttgatggg aacgggatgg aaagatgtat tttcgccaaa catttggcag gctgttttgc
      961 aaacgcagtt tggtggcgtc tggttatggc aaattgttct cgccctcgta acgttgatcg
     1021 tcgcccttat gcaaccgcgg aatatgccac gtttgctgtt tatgctcact accgcgcaat
     1081 ttattctgct ggcgggagtg gggcatgcga cgctgaatga aggggtaacg gcgaaaatcc
     1141 atcagactaa tcacgcgatt cacctgattt gtgcggcggc gtggtttggg ggattactgc
     1201 cggtgctctg gtgtatgcaa ctcatcaaag gccgttggcg acaccaggct attcaggcgc
     1261 tgatgcgttt ttcctggtgc gggcattttg cggtgatcgg cgtactggca agcggcgtgc
     1321 ttaatgcatt gttgattacc ggatttcccc ccacgcttac gacttactgg ggccaactct
     1381 tattgctcaa agccatcctg gtgatgatta tggtggtcat tgcgctggct aatcgttatg
     1441 ttcttgtacc gcgcatgagg caggatgaag atcgtgcggc accctggttt gtgtggatga
     1501 ctaaactcga atgggcgata ggtgcggtag tgctgctgat catcagcctg cttgcgactc
     1561 tcgaaccttt ttgatggatt acgaaaatga tgaaaaaaag tattctggcg tttctgttac
     1621 tcaccagttc tgcagcggcg ctggctgcac cgcaggtgat taccgtcagc cgttttgaag
     1681 tgggtaaaga caaatgggcg tttaatcgcg aagaggtgat gctgacttgc cgaccgggta
     1741 atgctttgta tgtcatcaac ccaagtaccc tcgtgcagta tcctttaaac gatgtcgcac
     1801 agaaggaagt tgccagtggg aagactaaag cccaacccat ttcggtgatt cagattgatg
     1861 atcccaacaa tcccggcgaa aaaatgagtc tggcaccgtt tatagaacga gctgaaaaac
     1921 tctgttaatt acctaaaata gccttttgat ttccaataaa aaaaccgcct cagttctttc
     1981 accagaacgg gcggttttta acatttaagc tgatgaccac cgcgcttttt attgaccatt
     2041 ttgcacgcaa actggaaaac ctggcgtcgt catctattct taaagggcaa ggcaactaag
     2101 cctgcattaa tgccaacttt tagcgcacgg ctctctccca agagcca
//
LOCUS       DBFIFI010000366         2141 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.368, whole genome shotgun sequence.
ACCESSION   DBFIFI010000366 DBFIFI010000000
VERSION     DBFIFI010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.368"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            255..470
                     /locus_tag="N7B39_004860"
     CDS             255..470
                     /locus_tag="N7B39_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HGU2424664.1"
                     /translation="MHQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            475..819
                     /locus_tag="N7B39_004861"
     CDS             475..819
                     /locus_tag="N7B39_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HGU2424665.1"
                     /translation="MKKKYELGVKGINNYPDKITVTVAPEIGGYPSLLLPDVAISLDR
                     TEGATLEFYEAEAKKQAKQFFMDVAAGLCEGDGPLPEKRPVILEAQDVLITYRGKLPG
                     IITGSLKTPPLA"
     gene            870..1403
                     /gene="rrrQ"
                     /locus_tag="N7B39_004862"
     CDS             870..1403
                     /gene="rrrQ"
                     /locus_tag="N7B39_004862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGU2424666.1"
                     /translation="MNAKIRYGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDGKPVVPGMKLSKEKCDQVNAIERDKALEWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            1559..1741
                     /locus_tag="N7B39_004863"
     CDS             1559..1741
                     /locus_tag="N7B39_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310767.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424667.1"
                     /translation="MGKYKRILLRLTMKNGLELKAPVTDDVSRALAFAIKWVAVGIAV
                     SPMLYGLAKLVIALKS"
     gene            1754..1885
                     /locus_tag="N7B39_004864"
     CDS             1754..1885
                     /locus_tag="N7B39_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424668.1"
                     /translation="MSDKLITLAKILCVIVGISFSLMLVAICFSTAWRVLTLSWLVG"
     gene            1893..>2141
                     /locus_tag="N7B39_004865"
     CDS             1893..>2141
                     /locus_tag="N7B39_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system i-spanin subunit Rz"
                     /protein_id="HGU2424669.1"
                     /translation="MNRVLCVVIIVLLVACGVLSLGLNHYRDNAITYKAQRDKKASEL
                     KLANVTITDMQVRQRDVAALDARYSRELADARAENETLR"
BASE COUNT          536 a          438 c          630 g          537 t
ORIGIN      
        1 ctggctaaga aggatagttg ggtttcacat gatacttata tctggcagta cattttctga
       61 cagacagtga tgggtgttgt caagatattg tgtcatttat aacctgaatc agggggggga
      121 gccggaatgt tatctggcat ttttagcaga gcctgaatgc cataatcacg gctcccggcg
      181 ttggccgtca gtgggcgaca ctggcggctt tttgttttcc tttactttca ttttctgtcg
      241 gcggtgacgg agacatacat cagatggaaa aaatcacaac aggtgtgtca tacaccacgt
      301 cagcggtggg gacgggatac tggttactgc agctgctgga caaagtctct ccgtcccagt
      361 gggtggcaat cggtgtgctg gggagtctgc tgtttggcct gctgacgtat ctgactaacc
      421 tgtatttcaa aatcagagag gaccgtcgta aggcggcgcg gggggagtaa agcgatgaag
      481 aaaaaatacg aactgggtgt taaagggata aataattacc cggataagat tactgttact
      541 gtggcaccgg aaattggtgg gtatccgtca ctgttgttgc cagatgtggc gattagtctt
      601 gaccgtactg aaggtgccac gctggagttt tacgaagctg aggcgaaaaa gcaggcgaag
      661 cagtttttca tggatgttgc tgccgggtta tgtgaagggg atggtccgtt gccggaaaag
      721 cgccccgtaa ttttagaggc gcaggatgtg ttgataacct acagaggaaa actaccggga
      781 ataattacgg gttctctgaa gactccaccg ctggcctgaa gacttaacat atccagggat
      841 ttgaaatcga taaaccctga taaatatcca tgaacgcaaa aatcagatac ggcctgtcgg
      901 ctgccgttct ggcgctgatt ggtgcagggg cgtctgcgcc tgaaatcctc gaccagtttc
      961 ttgacgaaaa agaaggtaac cacaccacgg cataccgtga tggtgcgggt atctggacca
     1021 tctgccgcgg tgccatcctg gtggatggta aacctgtcgt tccgggcatg aagttgtcga
     1081 aggaaaaatg cgaccaggtt aacgccattg aacgtgataa ggcgctggag tgggtggagc
     1141 gcaatattaa agtaccgctg accgaacccc agaaagcggg gatcgcgtca ttctgtccgt
     1201 acaacattgg tcccggtaag tgtttcccgt cgacgtttta cagacgaatt aatgcaggtg
     1261 atcgcagggg agcgtgtgag gcgattcgct ggtggattaa ggacggtggc agagactgcc
     1321 gtattcgctc aaacaactgc tacggtcagg tttcacggcg tgaccaggag agcgcgctgg
     1381 cgtgctgggg aattgacaga taagcagaat attttgctga aaaatgcggt ttgctcacac
     1441 tggcggataa cacgaaatcc tgcgaactgg caaaaactaa gtgaataaaa gtaaaacccc
     1501 gtttgttggc cgcaaacggg gttttgtgtt tctgaccttg agtaaggcaa gggagaacat
     1561 gggaaagtat aaacgaattc tgttgagatt gactatgaaa aacggccttg aactgaaagc
     1621 gcctgtaact gatgacgtca gcagagcgct ggcttttgct attaagtggg tggcggtcgg
     1681 tattgctgtg tctccgatgc tgtatgggct ggcaaaactg gtcattgcgt tgaaatcgtg
     1741 aagaggatta agcatgtcag acaagctcat aacgctggcg aagatcctct gtgtaatcgt
     1801 cggcatttca ttttcactaa tgctggttgc tatttgcttt tccactgcct ggcgcgtctt
     1861 aactttgtca tggctggtgg ggtgaggggg atatgaaccg tgttctgtgt gtggtgatta
     1921 ttgtcctgct ggtagcctgt ggtgtgctta gtctggggct gaatcattac cgcgataacg
     1981 ccattaccta caaagcgcaa cgcgataaaa aagccagtga gctgaagctg gcgaacgtga
     2041 caattactga tatgcaggta cgccagcgtg atgtcgctgc acttgatgcc agatactcga
     2101 gggaattagc cgatgcgaga gctgaaaatg aaactctgcg t
//
LOCUS       DBFIFI010000367         2122 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.369, whole genome shotgun sequence.
ACCESSION   DBFIFI010000367 DBFIFI010000000
VERSION     DBFIFI010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.369"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            1..651
                     /gene="zinT"
                     /locus_tag="N7B39_004866"
     CDS             1..651
                     /gene="zinT"
                     /locus_tag="N7B39_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HGU2424670.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLAPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            994..1524
                     /locus_tag="N7B39_004867"
     CDS             994..1524
                     /locus_tag="N7B39_004867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HGU2424671.1"
                     /translation="MNRFSKTQIYLHWITLLFVAITYAAMELRGWFPKGSSTYLLMRE
                     THYNAGIFVWVLMFSRLIIKHRYSDPSIVPPPPAWQMKAASLMHIMLYITFLALPLLG
                     IALMAYSGKSWSFLGFNVSPFVTPNSEIKALIKNIHETWANIGYFLIVAHAGAALFHH
                     YIQKDNTLLRMMPRRK"
BASE COUNT          661 a          445 c          414 g          602 t
ORIGIN      
        1 ttggcgattc gtcttcacaa actggctgtt gctttaggtg tctttattgt tagcgctcct
       61 gccttttcgc atggtcatca ctcacacggc aaacccttaa cagaggtcga acaaaaagct
      121 gctaatggtg tttttgatga tgccaatgta caaaaccgaa cgctcagtga ctgggatgga
      181 gtctggcaat ccgtttatcc tttactgcaa agtggcaaac ttgcccccgt ctttcagaag
      241 aaagcggatg cagataaaac taaaacattt gctgaaatta aagattatta tcacaaaggc
      301 tatgcaacag atatcgagat gattggcatt gaggacggca ttgttgaatt ccatagaaat
      361 aatgaaacaa catcctgtaa atatgattac gatggataca aaatactcac ctataaatca
      421 ggcaagaaag gcgttcgtta cttattcgaa tgtaaagatc ctgaaagcaa agcccctaaa
      481 tatatacaat ttagcgatca tataattgca ccacgaaaat catctcattt tcacattttt
      541 atgggtaatg attcacagca atctttatta aatgaaatgg aaaactggcc aacgtattat
      601 ccatatcagt tgagtagcga agaagtggtc gaggaaatga tgtctcattg agtattctca
      661 tgataacgcc tcgatgccgc tttagtatgt catcataact gccactggtc catccacaaa
      721 cgccactgaa cgcaagctag ctacagacac gctcatcact atgacgtgtc tgtatattaa
      781 taagctaacc cgcattgagt taaccaataa cggattccat acacaatacg gccaacagag
      841 aaagatacct gtgctcacgc cattgcttat attggctggt tacaatgtgc actatcaatt
      901 ttttaaataa caaattctta aaaccacatc tgtataagga atttttaagg ttcgtgggat
      961 agcttgactg tgaaaattac aggagctaca aaaatgaacc gattctcaaa aactcaaatt
     1021 tatttacatt ggataacgct gcttttcgtt gcaataacct atgccgcgat ggaactccgt
     1081 ggctggtttc ctaaaggtag tagtacttat ctgctgatgc gagaaacaca ttacaatgcg
     1141 ggtatattcg tttgggtgtt aatgttttca cgcctgatta taaaacaccg ttatagtgat
     1201 ccttctattg tgccaccgcc acctgcctgg caaatgaaag cggcttcgct aatgcacatc
     1261 atgctttata taaccttcct tgcgcttcct ctgctgggga ttgctttgat ggcttacagt
     1321 ggaaaatcgt ggagtttcct tggtttcaat gtgtctccct ttgttacccc aaacagcgaa
     1381 attaaagcac tgataaaaaa tattcacgaa acctgggcaa atataggcta ctttttaatc
     1441 gtagctcacg ctggcgcagc actctttcat cactacattc agaaagataa tactctgtta
     1501 cgaatgatgc ctcgccgcaa ataagtcgat cctctgacaa aggaaatgtg aaccatggac
     1561 atcaacgcat cataaatgtt tacgcaagtg cataccgcac tctttccact atcaacaagg
     1621 aggagcagaa aatgtgtaaa tcgtacaatg gtaagactta tcaaaaatac aggttaattt
     1681 tggtatttaa acctttttta cttgttagta tgatacaaag gctttcaaaa aagctgggaa
     1741 attcaacaac tcttggaaat tatggacttt gtcccgcggc tttaatctga cggccgcgtt
     1801 ccttttttga ctcaattctt gaccccttct ctacgcaact tcagttccca ccaccaactt
     1861 tgcggcagtt tcataggatg aatgtctaaa agaataatgg tgagcgataa gaaaacgact
     1921 gaataactgc acattttcgc tcgaaagctt cctgtcagat ccatagcgaa tcaagtgctg
     1981 aatgtcacag tatcgaacag aaaacagtga cgatctaacc cttcaagaat attctacgat
     2041 tgttctgttt aggaaaagca aggcgggaag tcgggagata agtcattgat aaagtggcgg
     2101 agagaggggg atttgaaccc cc
//
LOCUS       DBFIFI010000368         2081 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.370, whole genome shotgun sequence.
ACCESSION   DBFIFI010000368 DBFIFI010000000
VERSION     DBFIFI010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.370"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..463)
                     /locus_tag="N7B39_004868"
     CDS             complement(<1..463)
                     /locus_tag="N7B39_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HGU2424672.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEF"
     gene            complement(515..2081)
                     /locus_tag="N7B39_004869"
                     /pseudo
     CDS             complement(515..2081)
                     /locus_tag="N7B39_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310130.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
BASE COUNT          538 a          438 c          475 g          630 t
ORIGIN      
        1 taaactctac ttgatatgat ttcagacgct cgatggctgc tatcactttt gcaaaagtac
       61 cgttacctga attactgcgt ctgtaacggt catgtagctc ctggggtcca tcgatcgaga
      121 taccaaccag aaactcatgt tctttgagaa aggcacacca ttcattattc aataaaatgc
      181 cattcgtttg taatgcatta aaaatacgtt tttggcctgc atagcgttgt tgatagtgaa
      241 taactttacg gaaaaaatcc aggccagcca gagtgggttc accgccttgc caggtaaaat
      301 agacctgatt gccagacgct gcgatatatt gtttgatgaa ctctttcaga gtgctgtcat
      361 ccatccattt ttcatgagta aactgcgact ctttttcaag gtaaaaacag taatcacatt
      421 tgagattaca ttgaaaactg gagggcttgg ctgtaacgtg catcgctatc tcgctcaata
      481 aggcggcgga aaaatccgcc gcatgaaggt ttagttattt cgcttcgctt agtgctttct
      541 tgatattgtt aaacttctcc tgatttacct cgctaagcgg tggctggctg ctgtcgataa
      601 actctcttac cacgccttgc atctctttaa cgacctgcgg attggcggcg gcaaggttat
      661 ctttttgctg tagatccgtc agtttgtaga gacctaactg attgttttct actgtataga
      721 caagctaata atcgttattt ctcaccgtat aagagaattg gcttaagtcc tcagtgttgg
      781 ggttatgcgg gtaatcgtct gactgatgac ggacaaattt gtggtagtta tcccagaatg
      841 gaatattttc ctcatcaaac cagtgagaat aagaggttat ccaggtcaga tttttatgtg
      901 gctcgccttg tttcttatct tgcaaccagg gcagcaagga aacgccatcc agcttaaggt
      961 cttttggaat gctgatatcg gctgcatcaa gagctgtcgg gtagaaatcc attgcggaaa
     1021 tcagcttgtc ataattaccg ggttgaagtt ttcctttcca ccacataaac attggggtgt
     1081 gagtaccgcc aggataggtc tgactcttat agcctttttg cgccccgttc agcggcagag
     1141 gaccatcgat aaccgcacca ttatcggagg taaagagaat aattgtattg tcatactgtc
     1201 cgtttttctt cagttgttcg agaatgcgtt ttacaccctg atcaacagaa taaacggaag
     1261 cgtagtagtt atctgctgtt tgactaccgg tattaaattg cttctgatat tgctccggtg
     1321 caggattatc atttggcagg tgcggagcat tataagccag gtaaagcata aaaggctggt
     1381 caagtgtttt ggcacgatca acaacgccaa ttgcctcatc ggttaactga tcgctgatat
     1441 aaccttttgc ggggacacgt tcacgattct tgaacagtga aggggagttg taatatgccg
     1501 ttcctgcagc gtggaatccc ataaagtaat caaagccacg gttttgcggt tgccattctt
     1561 ccgcagaaaa tgtggtgaag ttgtcatgat agtcacgtgt ttgtttatct tccggtaccg
     1621 gcacattact gatttttgac aagtgccatt tacctactgc tgcagtgtaa taaccatgat
     1681 tctggaataa ttcaggcaag aaagtttctg ttagcggaat accatcctga gcatcagtat
     1741 tggaatagac accaaagcgg gcgggcgctc gaccggtcat tattgcggcg cgggaggggc
     1801 cggaaacacc gtgcgccaca tagccgttag taaaacgtac gccttcatcc attaatgaaa
     1861 ggagcgtcgg cgttgatttt tgcgcagctt caatggcttt atctatccct attttgtagg
     1921 tatcgacaac ttcacgattt tccattgttt ttgggtcaaa agatccctta tcaaaaggaa
     1981 gttgtccata accaagatca tccatggtca gtacgataat atttggcttt cctttggtac
     2041 tgtattcagt cggcgtaaag tctgagaaag caacgtttgt t
//
LOCUS       DBFIFI010000369         2076 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.371, whole genome shotgun sequence.
ACCESSION   DBFIFI010000369 DBFIFI010000000
VERSION     DBFIFI010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.371"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..183
                     /locus_tag="N7B39_004870"
     CDS             <1..183
                     /locus_tag="N7B39_004870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424673.1"
                     /translation="WRLAVVRCGWQQTITVTTRSPTKRSAIKKPESWNRRMPPLLTCR
                     CASVMLLRSMQNTRGN"
     gene            187..393
                     /locus_tag="N7B39_004871"
     CDS             187..393
                     /locus_tag="N7B39_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HGU2424674.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWAMMPP
                     SNSLRLLDETFSVSETESSATRQH"
     gene            458..682
                     /locus_tag="N7B39_004872"
     CDS             458..682
                     /locus_tag="N7B39_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424675.1"
                     /translation="MKKLLVTVKPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMVSAALQPVC"
     gene            1039..1179
                     /locus_tag="N7B39_004873"
     CDS             1039..1179
                     /locus_tag="N7B39_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424676.1"
                     /translation="MDFMLTVSGVVILSIAYTADKYGCHLLSRIGAYCSLMLIFSSLF
                     FE"
     gene            complement(1309..1494)
                     /locus_tag="N7B39_004874"
     CDS             complement(1309..1494)
                     /locus_tag="N7B39_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HGU2424677.1"
                     /translation="MSSKNRPRRTTIRNIRFPNHMIEQINIALEHKGSGNFSAWVIEA
                     CRRRLSTERSGMNYIIK"
     gene            1536..1901
                     /locus_tag="N7B39_004875"
     CDS             1536..1901
                     /locus_tag="N7B39_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HGU2424678.1"
                     /translation="MAVLRTLPGRIKTLNTRRINVLRGEQRRVSGSARVSLKRRIWRR
                     DAGHCCLCRRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSASEAASGMPD
                     PTLPELPDGTLRADGITGL"
BASE COUNT          488 a          466 c          549 g          573 t
ORIGIN      
        1 tggcggttgg ctgtggtgcg ctgtggctgg caacaaacca ttaccgtgac aacgcgctca
       61 cctacaaagc gcagcgcgat aaaaaagcca gagagctgga acaggcgaat gccaccatta
      121 ctgacatgca ggtgcgccag cgtgatgttg ctgcgctcga tgcaaaatac tcgagggaat
      181 tagccgatgc gagagctgaa aatgaaactc tgcgtgctga tgttgccgct ggtcgtaagc
      241 gcctgcggat caacgccacc tgccccggta ccgtgcgtga agccaccggc acctccggcg
      301 tgggcaatga tgccgccgtc gaactctctc cggttgctgg acgaaacgtt ctcggtatca
      361 gagacggaat catcagcgac caggcagcat tgagaatgct tcaggaatat atccgcactc
      421 agtgtattaa ctagtatttt tgttattcgg agaatgcatg aagaaattat tggtaaccgt
      481 aaagcctttt cagggaacaa ttccgttccg tattttgcag cgtggtcgtg ttcttgttga
      541 aggttcgttc agtggtaaat gtacgcaatt acactccagg acctttcagg tgaatgccac
      601 gaatgaagag ctaaccgttg agtgtacgat gaatgccgct aaatgccgca tggtatccgc
      661 tgcattacag ccagtgtgtt gagcgacctt attattcatg cgcggtattg tcgccgtatt
      721 cctgcattaa cagagaccgc agcccgaccg ggagaatcct ctgcgcgagt gtgcggggat
      781 aatcaaaaac gatacacacc ggggtttacc gcgttaacgg agcgcggcgt tgtcccctca
      841 tggtcgctgg tccggtgcga tggtggaaga agccggatgt ttatcactat taattgataa
      901 cacagaaatg gattcattga ttttcagcac gtttttgtat tcgtattatt gaacatctgt
      961 ttattttact tttaacatat tgataataaa aagagctgta aatctttaga tgagtcgatt
     1021 ttgtccgggg aagttcaaat ggattttatg ctgacggttt ctggtgtggt tatcctgtcc
     1081 attgcttata ctgcagataa atatggctgc catttgttat cacgtattgg cgcttattgc
     1141 tcgttgatgc tgattttctc gtcgcttttt tttgagtaag ttatattaat tataacaaat
     1201 aattttctgt gttttttcag gctatcccgt cagacgggaa gcctgtactg ccgggggacg
     1261 aatggaaaac tgatgtgtcc ggtaactgtg tgttctgtga acaccatgtt acttaattat
     1321 gtaattcata cccgaacgct ctgttgacag ccttcttctg caggcttcaa taacccacgc
     1381 tgaaaagtta ccggaacctt tatgttcaag ggcgatattg atctgttcaa tcatgtgatt
     1441 ggggaaacgg atattgcgga ttgtggttct gcggggccgg tttttcgatg acattttctt
     1501 tcctctggtg acaagctata tggcgaggat tttacatggc tgtgcttcgt acgttaccgg
     1561 gcagaatcaa aactctgaac acccggcgga taaatgtcct gaggggggaa cagcgtcgtg
     1621 tcagtggcag tgcccgggtt tccctcaaac gtcgtatctg gcggagggat gccggacact
     1681 gttgtctctg tagacgcgtg gttgaccttt gtgacagtga actcgatcac cgcattgcac
     1741 ttcagttcgg tggtggtaat gaggagacga acctctggac cctctgtact gaatgccatc
     1801 gccagaagtc agcgagtgaa gcggcgagtg gtatgcctga tccgacgttg cctgagcttc
     1861 ctgatggcac gctcagggcc gacggaatca ctggcctgtg accagacccg ggggggatca
     1921 tccggcgaaa aaaaacgatc gccctggaca ccgcgccccc tctcacgcag agaaaaaatt
     1981 cccgtttcag ggcagttaac atgttaactg gctgcccggg catttttgcg gtttttatct
     2041 ttattattca gtttgttgtg cggaaaaaat gttaac
//
LOCUS       DBFIFI010000370         2054 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.372, whole genome shotgun sequence.
ACCESSION   DBFIFI010000370 DBFIFI010000000
VERSION     DBFIFI010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.372"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..426
                     /locus_tag="N7B39_004876"
     CDS             <1..426
                     /locus_tag="N7B39_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HGU2424679.1"
                     /translation="NDARTLFEFTSGVNVIESPIIYRDESMRTACSPDGLCSDGNGLE
                     LKCPFTSRDFMKFRLGGFEAIKSAYMAQVQYSMWVTRKDAWYFANYDPRMKREGLHYV
                     VVERDEKYMASFDEMVPEFIEKMDEALAEIGFVFGEQWR"
     gene            423..605
                     /locus_tag="N7B39_004877"
     CDS             423..605
                     /locus_tag="N7B39_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HGU2424680.1"
                     /translation="MKHPHDNIRVGAITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEE
                     INNKRGAVCTKHLPLS"
     gene            578..769
                     /locus_tag="N7B39_004878"
     CDS             578..769
                     /locus_tag="N7B39_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HGU2424681.1"
                     /translation="MHKASPVELRTSIGMAHSLAQIGVRFVPIPVETDEEFHTLAASL
                     SQKLEMMAAKAEANERDPA"
     gene            846..1061
                     /locus_tag="N7B39_004879"
     CDS             846..1061
                     /locus_tag="N7B39_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HGU2424682.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQQFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            1160..1381
                     /locus_tag="N7B39_004880"
     CDS             1160..1381
                     /locus_tag="N7B39_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HGU2424683.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNYQTVSATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEDLELISKQRGSK"
     gene            1378..>2054
                     /locus_tag="N7B39_004881"
     CDS             1378..>2054
                     /locus_tag="N7B39_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HGU2424684.1"
                     /translation="MSEINYQALREKAEKATKGSYIVGHTSVNQHGNLTGVFVCQKWK
                     GEPGGVIAECHVNCLIESDAQAYANAEFIAEANPATVLELLDERERNQQYIKRRDQEN
                     EGIALTVGKLRVELEAAKSKLNEQREYYEGVIADGSKRIAELEKQCAEWERKALSNFE
                     ECAAMAERIEEMQTKSAPDSFGIIGENIRTQDNRITSDPMFCVYQKREIAVDADYDHD
                     RIVWVDE"
BASE COUNT          610 a          433 c          543 g          468 t
ORIGIN      
        1 aacgacgcca gaaccctgtt tgagttcact tccggcgtga atgttattga atccccgatc
       61 atctatcgcg acgaaagtat gcgtaccgcc tgctctcccg atggtttatg cagtgacggc
      121 aatggccttg agctgaaatg cccgtttacc tcccgggatt tcatgaagtt ccggctcggt
      181 ggtttcgagg ccataaagtc ggcttacatg gcccaggtgc agtacagcat gtgggtgaca
      241 cgaaaagatg cctggtactt tgccaactat gacccacgta tgaagcgtga aggactgcat
      301 tatgtcgtgg ttgagcggga tgaaaagtac atggcgagtt ttgacgagat ggtgccggag
      361 ttcatcgaaa aaatggacga ggcactggct gaaattggtt ttgtatttgg ggagcaatgg
      421 cgatgaagca tcctcacgat aatatccggg taggtgcgat cactttcgtc tactccgtta
      481 caaagcgagg ctgggtattt cccggccttt ctgttatcag aaatcccctg aaagcacagc
      541 ggctggctga ggagataaat aataaacggg gagctgtatg cacaaagcat ctcccgttga
      601 gttaagaacg agtatcggga tggcacatag cctcgctcaa attggagtca ggtttgtgcc
      661 aataccagta gaaacagacg aagaatttca tacgttagcc gcatcccttt cacaaaagct
      721 ggaaatgatg gcggcgaaag cagaagcaaa cgagagagac ccggcatgac aacaacagaa
      781 tgcatttttc tggcagcagg cttcatattc tgtgtgctta tgcttgccga catgggactt
      841 gttcaatgac acctcagcag gaaaacgccc ttcgcagcat tgcccgtcag gctaattctg
      901 aaatcaaaaa agccagacag cagtttccgg ataaaaacgt cgatgacatt tgccgtagcg
      961 tactgaagaa gcaccgcgaa acggtaacgc taatgggatt cacaccgact catttaagtc
     1021 tggcgatcgg catgttaaac ggcgtcttta aggagcgatg aacatgaaaa gcaaaatcat
     1081 cagggagcta caggctcctt ttttattgtt cgcattcacc ctcaagcgta ttaaccaaca
     1141 attcagggat taatgaaaga tggcagatat cattgattca gcatcagaaa ttgaagaatt
     1201 acagcgcaat acagcaataa aaatgcgtcg cctgaactac cagactgtat ccgcaactca
     1261 ttgttgtgag tgtggcgatc cgatagatga acgaagacgc ctggcagttc agggttgtcg
     1321 gacttgtgca agttgccagg aggatctgga gcttatcagt aaacagagag gttcgaagtg
     1381 agcgaaatta attatcaggc actgcgtgaa aaggcagaaa aagcaactaa aggaagctac
     1441 atcgtagggc atacatctgt taaccagcac ggcaatttaa caggagtttt tgtttgccaa
     1501 aaatggaaag gagaacccgg tggcgtgatt gcggaatgtc atgttaactg cctgattgaa
     1561 tcagatgctc aggcttatgc aaacgctgaa ttcatagcag aggctaaccc ggctaccgtg
     1621 ctggaactgc tggatgaacg ggaaagaaac cagcaataca tcaaacgccg cgaccaggag
     1681 aacgagggga ttgcgcttac ggttgggaag ctgcgtgttg agcttgaagc agcaaaatca
     1741 aaactcaacg agcagcgtga atattacgag ggagtaatcg cggatggaag taagcgcata
     1801 gcagaactgg aaaaacaatg cgccgaatgg gagcgaaaag cattaagcaa ctttgaagag
     1861 tgtgctgcga tggctgaacg tatcgaagag atgcagacaa aatctgcacc agattcgttt
     1921 ggcatcatcg gtgaaaatat tcgaacacag gacaatcgaa taacgtcaga tcccatgttt
     1981 tgtgtgtatc aaaagcgcga aatcgctgtt gatgctgatt atgaccatga ccggattgtc
     2041 tgggttgacg aaga
//
LOCUS       DBFIFI010000371         2034 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.373, whole genome shotgun sequence.
ACCESSION   DBFIFI010000371 DBFIFI010000000
VERSION     DBFIFI010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.373"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..1330)
                     /locus_tag="N7B39_004882"
     CDS             complement(<1..1330)
                     /locus_tag="N7B39_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HGU2424685.1"
                     /translation="MNISNSQIDILRRDVRAGLRALFRPEPQTAVEWADASYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNPL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLSLAPWYGKKHRDNTLTMKRFTNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEYYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDE"
     gene            complement(1305..1850)
                     /locus_tag="N7B39_004883"
     CDS             complement(1305..1850)
                     /locus_tag="N7B39_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HGU2424686.1"
                     /translation="MKVNKKKLAEIFNVDPRTIERWQSQGLPCVSGGGKGVESVFDTA
                     TAIQWYAQREADIENEKLRKEVEDYRAASEADLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSD"
BASE COUNT          473 a          584 c          486 g          491 t
ORIGIN      
        1 cctcatccac acgcagcagc gtctgttcat cgtcgtggcg gcccataata atctgccggt
       61 caatcagcca gctttcctca cccggccccc atccccatac gcgcatttcg tagcggtcca
      121 gctgggagtc gataccggcg gtcaggtaag ccacacgatc aggaacgggc gctgaataat
      181 actctttccg ctctgccatc acttcagcat ccggacgttc gccgattttc gcttcccacg
      241 tctcaccgag cgtggtgttc acgaaggttt tacgttttcc cgtatcccct ttcgtcttca
      301 tccagtcttt gacaatctgc acccaggtgg tgaacgggct gtacgccgtc cagatgtgaa
      361 aggtcacact gtcaggcggt tcaatctctt caccggatga cgaaaaccag agaatgccat
      421 cacgggtcca gatcccggtc ttttcgcaga tataacgggc atcagtaaag tccagctcct
      481 gctggcggat gacgcaggca ttatgctcgc agagataaaa cacgctggag gggtcatccg
      541 gcgtccattt gaggccaaac ggcgtctctt tgtcgccaaa tttaagatac tgctcctccc
      601 cgcagtgcgg gcaggcaaca tgaaaacgca taaaatgcgg ggattcactg gctgcacgct
      661 caatctggca ggtgcctctc actttgggcg tggagccacg gatggacttt ggccagaccg
      721 agccttcaat acgcttgtca cccaggaacg tcggagagcc ttcctgttca atatcctcat
      781 caaaggcagc aagttcatca taacccgcca catcaaccga cttttcacgg tagttttttg
      841 ccgctttacc gcccaggcac cagaagccac gaccattggt gaaacgcttc atagtgagcg
      901 tgttatcccg gtgctttttg ccataccacg gggccagaga cagcagcgac ggaatatcgc
      961 ggatggtagg ctcgacgtgg gttttcataa agttctcggc atcaccatcc gtcggcaacc
     1021 agataagggg gttacgctgc ttatgctcta tgaagtaggc ataaacaccc agcagcattt
     1081 ttgaataacc aacacgggca gacttcacca cattcacctc gcggatgtag tcgctgccca
     1141 tcgcattcat gatagcccgc tgaaagggta gtgtttccca gcgcccttcc tggtatgcgg
     1201 attctttcgg gagatagtaa ctggcatccg cccattcaac ggcagtctgt ggctccggcc
     1261 tgaacagggc tcgcagcccg gcgcgtacat cacgccgcag aatatcaatc tgactgttcg
     1321 atatattcac tcagcaaccc cggtatcagt tcatccagcg cggctgcttt gttcatggct
     1381 ttgataatat cccgtttcag gaaatcaaca tgtcggtttt ccagttccgg aaaacgccgc
     1441 tgtactgaga gagggatccc gtcaagaata ctggcaattt cacctgcgat ccgtgacagc
     1501 acgaaagtac agaatgcggt ttccaccact tctgcggagt ctctggcatt cttcagctcc
     1561 tgggcgtcag cctgcgcacg cgtaagtcga tggcgttcgt actcaatagt cccaggctgg
     1621 agatctgcct cgctggcagc cctgtaatcc tcaacctctt tacggagttt ttcattttcg
     1681 atatcagctt ccctctgcgc ataccactga attgccgtgg cggtatcaaa tacagattca
     1741 acgcccttac cacctccgga gacgcaaggg agcccctgag actgccagcg ttcaatcgtt
     1801 cgcggatcca cgttgaaaat ttcggcaagt ttctttttat taaccttcat gaaacagtct
     1861 cacaacaaat acagggtccg acatgaaagt gcccgaaaat gactttttca ggcgttttca
     1921 tgtcggacct ttacggattc gatattagaa aaaacaaata gttatgttcg agaagtaccg
     1981 acatgatttt ccccggaaaa ttttcataaa tagcgaaaac ccgcgaggtc gccg
//
LOCUS       DBFIFI010000372         1988 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.374, whole genome shotgun sequence.
ACCESSION   DBFIFI010000372 DBFIFI010000000
VERSION     DBFIFI010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.374"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            1..276
                     /locus_tag="N7B39_004884"
                     /pseudo
     CDS             1..276
                     /locus_tag="N7B39_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310990.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
     gene            441..1679
                     /locus_tag="N7B39_004885"
     CDS             441..1679
                     /locus_tag="N7B39_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU2424687.1"
                     /translation="MSVKPLTVTEVKGMKPREKDYAVYDGFGLLLNVSKAGGKVWRFR
                     YSHPITKKRQTYTIGRFPEFSLAEAREVRDELRRMIARGVDPVTEKKNRKIEMSLKNL
                     QTFEAVANAWFAFKKGSELRKPTLYNIEYEVYKYLVPFFGKYSIEKITAPVAINALDA
                     VSDKNALQKKLISRLNEIMNYAVNCGALKANPLLKIKTAFTGKKNKSLAALPVERLPE
                     FLSWWDSVPHTYQIAHNALLFQILTMVRPGEAIKAEWSEIDFDSGLWIIPAHKMKCHR
                     EHVVPLSSQAISILRTMQEIKRGRYVFFSSRTKDAPMGRNTIKTPIAASKFKGIVTLH
                     GFRSMWSTLLNEEGFNPDVIEAALAHKSGDKIRDIYNRTIYLEQRKIMMQWVGDFFDD
                     ARKGVINRSGGMKGLRVVNG"
     gene            1693..1896
                     /locus_tag="N7B39_004886"
     CDS             1693..1896
                     /locus_tag="N7B39_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU2424688.1"
                     /translation="MNTNEDILFTKDVMKILRYGAMSAFINFWKDENNGFPQPFRIGR
                     RHTWHRRDVEAWLDKQREQANPH"
BASE COUNT          603 a          338 c          498 g          549 t
ORIGIN      
        1 ggagaccgcc gccggtggta ctggccgtgt ccgcactgcg gggaatattt tcagccggtg
       61 atggataaca tgaccggata ccggaataac ccggattttg tggctgccgg tcaggctgcc
      121 cgtctgatgt gtccgcattg tcgcgggctg attgcccctg agcagaaacg cgaactgaat
      181 aaccagggga tctggcttcg tgaaggtgaa cgggcggcgg cggacggcag tatcaccgga
      241 acgccacgaa actcccggat tgcgtcattt tggatgtagg gtacatttct tacctgtttg
      301 tatgttctgg tgtcgtttca tagtcttttc aatgagttgt gattttatga gtttcctctc
      361 tttgcttgat aatgagttag tttatcgctt gttattggct tgaatggact acatgacgga
      421 ctaaaaaatg agggcgatag atgtcggtaa agccattaac cgtgactgaa gttaagggga
      481 tgaaaccacg tgaaaaggac tatgccgttt atgatgggtt cggtttattg ctgaatgtga
      541 gtaaagccgg tgggaaagtg tggcgtttcc gttatagcca tccgataacg aagaaacggc
      601 agacatacac gataggacgt tttcctgaat tctcactcgc ggaagcacgg gaagtacgtg
      661 atgaacttcg gcgaatgatt gcacgtggag ttgatccagt gacggagaag aaaaatcgta
      721 aaattgagat gtcactaaaa aatctacaga catttgaagc tgttgctaat gcatggttcg
      781 cttttaaaaa gggatctgaa ttgcggaaac ctacgctgta taatatcgaa tatgaagtat
      841 acaaatatct tgttcctttc tttggtaagt acagtataga aaaaattaca gcaccagtag
      901 ctattaatgc tctggatgcc gtatccgata agaatgcgtt gcaaaaaaaa ttaatatcaa
      961 gattaaatga aattatgaat tatgctgtaa attgtggagc attgaaagca aatccattac
     1021 ttaagataaa gactgcattc acaggaaaga aaaataaatc attagcagca ctacctgttg
     1081 aaagattgcc tgaatttctg agctggtggg atagtgtgcc tcatacctat caaatagctc
     1141 ataatgcact tttattccag atattgacaa tggtcaggcc aggtgaggcg attaaagcag
     1201 agtggtcaga gattgatttt gattctggct tgtggattat ccccgcgcat aaaatgaaat
     1261 gccatcgtga acatgttgtt cccttgtcat cacaggctat tagtatcctc agaacaatgc
     1321 aggaaataaa aagagggcgt tatgtgtttt tttcctccag aacaaaagat gcgcctatgg
     1381 ggaggaatac tatcaagacc ccaattgctg ccagtaagtt caaagggatt gtaacgttac
     1441 atggttttcg ttcaatgtgg agtacgcttt taaatgagga gggatttaac cccgatgtaa
     1501 tcgaggctgc attggcgcat aaaagtggtg ataaaataag agatatttat aatagaacta
     1561 tttatctaga acagcgtaag atcatgatgc aatgggtcgg tgattttttt gatgatgcga
     1621 gaaaaggggt aattaataga tccggtggta tgaaaggttt aagagtagta aatggttgag
     1681 gaggttcagc aaatgaatac caatgaagat attttattta ctaaagacgt aatgaaaatc
     1741 ttgcgatatg gagcaatgag tgcattcatc aatttctgga aagatgagaa taatggtttt
     1801 cctcagccgt tcagaattgg acgacgacat acctggcacc gtagagatgt agaagcatgg
     1861 ttagataaac aacgagaaca ggccaatccc cactaataat atctttcata ccccgcgtgc
     1921 aatgcggggt tttttgtatg tgaggtaaaa aagaaatgaa taaaaatatt gccgtgacgg
     1981 gcaagggt
//
LOCUS       DBFIFI010000373         1969 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.375, whole genome shotgun sequence.
ACCESSION   DBFIFI010000373 DBFIFI010000000
VERSION     DBFIFI010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.375"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(187..1086)
                     /locus_tag="N7B39_004887"
     CDS             complement(187..1086)
                     /locus_tag="N7B39_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU2424689.1"
                     /translation="MKIDLNLLPIFIAVAEESNFSKAAARLGVTRSAVSQGIRRLEDA
                     FGTMLVMRTTRSVNLTEAGERLHKSLSLPLSGIEAAFEEMVSDHVPRGLLRIAVTSIA
                     EEFLSGPLIASFAAANPAVTLDIVVTDEEFDIVAAGYDAGVRLGEVIEKDMIAVPLTG
                     RQREMVVASPSYLAANSTPVHPRELVNHKCIGWRQSPEVAPYRWPFEENGRAFDLAIE
                     PQITTNDLRLMLRLALAGGGITIATQETFRPYIESGKLVSLLDDFLPQFPGFYLYFPQ
                     RRNIAPKLRALIDHVKEWRQQLA"
     gene            1185..1907
                     /locus_tag="N7B39_004888"
     CDS             1185..1907
                     /locus_tag="N7B39_004888"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU2424690.1"
                     /translation="MDKVILITGASSGIGEGIARELGMTGAKVLLGARRVERIEAIAT
                     EICRAGGIAKARELDVTDRQSMADFVQAALDSWGRVDVLINNAGVMPLSPLAAGKQDE
                     WALTIDVNIKGVLWGIGAVLPVMEAQGSGQIINLGSIGALSVVPTGAVYCASKFAVRA
                     ISDGLRQESSKIRVTCVNPGVVESELASTITHVETMKAMDAYRSVALQPADIARAVRH
                     IIESPESVDTTEITIRPTASAN"
BASE COUNT          529 a          460 c          474 g          506 t
ORIGIN      
        1 ttaatttatg tattaaaatc attgtgttgg tggcgattat tggttcctat tatcgcacca
       61 tttaaatcaa gtagttaccc catatttaaa tacaccacgt ttcctcctgt gccgcatttg
      121 tgccattgta accctggcaa ttaatcaaaa tactgttctg acatcaggca atgcaggtgc
      181 agacatttaa gccaattgct gccgccattc cttaacgtgg tcaatcagag cgcggagctt
      241 tggtgcaata ttgcgacgct gtgggaaata cagatagaag cccggaaatt gtggaagaaa
      301 gtcatcaagc agagatacaa gcttaccgct ttcaatatac ggcctgaaag tttcctgagt
      361 ggcaattgtt attcctccgc cggcaagagc cagcctcaac atcagacgca gatcattagt
      421 cgtaatctgc ggttcaatcg caaggtcgaa agctctcccg ttttcttcaa atggccagcg
      481 ataaggcgca acctccgggg actgacgcca gccgatacac ttatgattca caagttcacg
      541 cggatgaaca ggtgtgctgt tggctgcgag ataggaggga gaggcaacga ccatttcacg
      601 ctgcctgccg gtaaggggca cagcaatcat gtctttttca atgacctctc cgagccttac
      661 gccagcatcg taacctgcgg ccacgatatc gaattcctca tccgtgacaa caatatcaag
      721 cgtaacggct ggattggctg ctgcaaacga agcgattagc ggacctgaaa gaaattcttc
      781 agctattgac gtcaccgcga ttctgagaag tccgcgtggc acatggtcag aaaccatctc
      841 ttcgaacgct gcctcaatac cggataaagg taatgacagc gatttatgca gccgttctcc
      901 cgcttctgtc aaattaactg agcgcgtggt ccgcattaca agcattgtcc cgaatgcatc
      961 ttcaaggcgt cttattccct gactgaccgc tgaacgggta acgcccagcc gtgcggcggc
     1021 tttactaaaa ttactctctt ctgctaccgc gataaaaatg ggtaaaagat taagatcgat
     1081 tttcattgtt taatgccgct aaccactgag tacattgaaa gcaggatact ggaaccaatc
     1141 ataaagttct attctctctc cgaacacatt tgaaggagaa cgtaatggat aaggtcatat
     1201 taattaccgg cgcatcaagt ggtattgggg aaggtattgc cagagagctt ggaatgacag
     1261 gtgcaaaggt tttactggga gcacgcagag ttgagcgtat cgaagccatt gcaaccgaaa
     1321 tctgccgcgc aggaggaatt gctaaagcgc gggagttgga tgtcacagac cgacagtcca
     1381 tggccgattt cgtgcaagca gcgctggata gctgggggcg agttgatgtt cttatcaata
     1441 atgcgggcgt tatgccgctt tcaccgcttg cagcaggaaa acaagatgaa tgggcgctca
     1501 ctattgacgt gaatatcaaa ggtgtactgt ggggaattgg cgctgtactt ccggtgatgg
     1561 aagcacaggg ttccggtcag ataatcaatc ttggttctat tggtgccctt tctgttgtgc
     1621 ccacaggcgc agtctattgt gcatcaaaat ttgcagtacg ggctatttct gatggcttac
     1681 ggcaggaaag ctcgaaaatc cgtgtgacct gcgtaaatcc tggcgttgtc gaaagtgaac
     1741 tggcttcaac cattacccat gtggaaacca tgaaggcaat ggacgcctac cgttctgttg
     1801 cacttcaacc cgcggatatt gcccgtgctg taaggcatat tattgaatct cccgaaagcg
     1861 ttgatacaac cgaaattacc atcagaccca ctgcatctgc aaactgaaga ataactttta
     1921 cattcacgtt ttattaaata ggtcagaagg aatatttata tatccctgt
//
LOCUS       DBFIFI010000374         1968 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.376, whole genome shotgun sequence.
ACCESSION   DBFIFI010000374 DBFIFI010000000
VERSION     DBFIFI010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.376"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            75..209
                     /locus_tag="N7B39_004889"
     CDS             75..209
                     /locus_tag="N7B39_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001420444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HGU2424691.1"
                     /translation="MKLILGTACKTRHLVCKNVLRLAGELSIVRVLNDFQPLLILLVN
                     "
     gene            complement(206..448)
                     /locus_tag="N7B39_004890"
     CDS             complement(206..448)
                     /locus_tag="N7B39_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HGU2424692.1"
                     /translation="MPHRYPAAKINKMPKGSVPALQQEMLRRVSKRYDDVEVIIKSTS
                     NDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVR"
     gene            580..1863
                     /gene="ydfJ"
                     /locus_tag="N7B39_004891"
                     /pseudo
     CDS             580..1863
                     /gene="ydfJ"
                     /locus_tag="N7B39_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
BASE COUNT          517 a          400 c          473 g          578 t
ORIGIN      
        1 gtgcttttga ctctgacaga ggtaacttcg ttttaaaaaa ttaagatagc aatggcgggt
       61 taacttttcg taaaatgaaa ttaattctcg gtacagcatg taaaacgcgg cacctcgtat
      121 gcaagaacgt gctgcggttg gctggtgaac tttcgatagt gcgagtattg aatgatttcc
      181 agccgttact gattttactc gttaattagc gaacaaacca ctcgtcagca gactcccagg
      241 tatctttcag cgtctcctga acaaaagttt ttgcagaatc tttatcggcg gtgcgagtaa
      301 ctgaaaggcc atcgttgctg gtggatttga tgatcacttc tacatcgtca taacgtttac
      361 tgacacgtcg cagcatttct tgttgcaggg caggaacaga accttttggc attttgttga
      421 ttttggctgc ggggtatctg tggggcatat atgggacata aaaaggcctc aaataaacag
      481 caaaatcgag attagggatt tttagaaaaa atacaaccat ctgaaaaaac ttagaaaaca
      541 cccaaaaacc cacagaatag taaaaaacat ctatgaatta tggatttcca gttatattcg
      601 ctcggcgcag cgttagtgtt tcatgaaata ttttttcctg aatcatcaac ggcaatggcg
      661 ttaattctgg caatgggaac ctacggtgca ggttatgtgg cgcgtattgt cggagcattt
      721 attttcggca aaatgggcga cagaataggg cgtaaaaaag tgctctttat taccatcacc
      781 atgatgggga tctgtaccac cttaattgga gtgttaccga cctatgcaca gattggtgtt
      841 tttgccccca tcttgctggt gacgctgcgt attattcagg gattgggtgc aggtgcggaa
      901 atttccggtg ccggtacgat gctggcggaa tatgcgccaa aaggtaagcg cggaattatc
      961 tcctcatttg tggctatggg aactaactgc ggaaccttga gcgcaacggc aatctgggcc
     1021 tttatgttct tcattctcag taaagaggaa ctgctggcgt ggggatggcg tataccgttc
     1081 ctggcgagcg ttgtcgtgat ggtctttgct atctggttgc gtatgaatct gaaagaaagc
     1141 ccggtctttg agaaggttaa cgacagcaac caaccgacag caaaacctgc acctgctggt
     1201 agcatattcc agagcaaatc cttctggctg gcaacagggc tgcgttttgg tcaggcaggt
     1261 aactccgggt taattcagac tttccttgca ggctatttag tacagacgtt attgtttaac
     1321 aaagcaattc caacagatgc attgatgatc agttcgattc ccggctttat gaccattccg
     1381 ttccttggtt ggttatccga taaaattggt cgccggatcc cgtatattat tatgaatacc
     1441 tccgcgattg tgctggcatg gccaatgctt tctatcatcg tagataaaag ctatgccccg
     1501 agcaccatta tggttgcact gattgtgatt cataactgtg cggtgctggg attatttgct
     1561 ctggaaaaca ttaccatggc agaaatgttc ggctgtaaaa accgctttac ccggatggct
     1621 atttctaaag aaattggtgg tcttatcgct tccggttttg gtcctatcct ggcgggtatt
     1681 ttctgcacca tgacggaatc ctggtatccg atcgccatta tgatcatggc atattcagtg
     1741 attggtttaa tctctgcgct gaaaatgcca gaagtgaaag accgtgattt aagtgcgctg
     1801 gaagacgctg cggaagatca accgcgtgtt gtaagagctg cgcaaccttc cagaagtctg
     1861 taaatcctca atcccttctc ttaccggaga ggggattttt attcatataa caaaacatgt
     1921 agcttgccat atttaaattt aaggaattgt tatgggaaat aatttgtt
//
LOCUS       DBFIFI010000375         1938 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.377, whole genome shotgun sequence.
ACCESSION   DBFIFI010000375 DBFIFI010000000
VERSION     DBFIFI010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.377"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..132
                     /locus_tag="N7B39_004892"
     CDS             <1..132
                     /locus_tag="N7B39_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGU2424693.1"
                     /translation="SRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMAA"
     gene            83..784
                     /gene="traT"
                     /locus_tag="N7B39_004893"
     CDS             83..784
                     /gene="traT"
                     /locus_tag="N7B39_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HGU2424694.1"
                     /translation="MSPRQPSGKNIIRWLLKKRTNGSVRYCQYTSTIWLEPASERTVF
                     LQIKNTSDKDMSGLQGKIADAVKARGYQIMTSPDKAYYWIQANVLKADKMDLRESQGW
                     LSRGYEGAAVGAALGGGITAYNTGSAGTTLGVGLATGLIGMAADAMVEDINYTMITDV
                     QIAERTRTSVRTDNVAALRQGTSGSKIQTSTETGNQHKYQTRVVSSANQVNLKFEEAK
                     PHLEDQLAKSIANIL"
     gene            complement(832..1083)
                     /locus_tag="N7B39_004894"
     CDS             complement(832..1083)
                     /locus_tag="N7B39_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HGU2424695.1"
                     /translation="MESFVQDSPFYSGRDLYWLRPKVELTLEEKLYYCSCIRRNRHKY
                     SYGRQANRTLKNLLVPSLDSVPAWVYGVTGKIISELSER"
     gene            complement(1107..1397)
                     /locus_tag="N7B39_004895"
     CDS             complement(1107..1397)
                     /locus_tag="N7B39_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HGU2424696.1"
                     /translation="MNLSASMNDAGDGFELLMSGNMQDFNICCRRDNHYPEHKLIPLS
                     ELFDVNYGLNLELNKLEKDSSGINFVSRTSKNNGVSARVKLKDGISPCQQLC"
BASE COUNT          583 a          460 c          408 g          487 t
ORIGIN      
        1 agccgggatg aggccatctc agtaatacgg gaatacattg agattttcta caatcgtcag
       61 cgtcgtcact ctcgtctggg gaatatctcc ccggcagcct tcagggaaaa atatcatcag
      121 atggctgctt aaaaaaagaa caaatggtag tgtccgctat tgtcagtaca cctcaaccat
      181 ctggcttgaa cctgcgagcg aacgcaccgt tttcctgcag atcaaaaaca cgtcggataa
      241 agacatgagt ggactacagg gaaaaattgc cgatgcagtc aaagccagag gttatcagat
      301 catgacttcc ccggataaag cttactactg gatccaggct aacgttctca aagcagacaa
      361 aatggatctg cgtgaatctc agggatggct gagtcgcggt tatgaaggtg cagcagtcgg
      421 agctgctctt ggaggtggaa tcacagcgta caataccggg tcggcaggta ccactctcgg
      481 tgttggactg gctaccggtc tgattggcat ggccgcggat gcaatggtgg aagatataaa
      541 ttacacaatg attacagatg ttcagattgc cgagcgaacc agaaccagcg tcagaacgga
      601 taatgttgca gctttgcgtc agggaacatc aggctccaaa attcagacaa gcacagaaac
      661 cggcaaccag cataaatacc agacccgggt tgtatccagt gccaaccagg ttaatctgaa
      721 gtttgaagaa gctaaacctc atctggaaga ccagctggca aaatcaatcg ccaatatact
      781 ctgatataaa tatcccggtg gccattcacc tggcctccgg ccctcttagt ctcaacgttc
      841 agacaattca gatattattt tccctgtcac accatatacc catgccggaa cagaatctaa
      901 tgacggtacc agtaaattct tcagcgttcg gtttgcctga cgcccgtagc tgtatttgtg
      961 tctgtttctt cttatacaag agcagtagta caatttctct tccagagtca gttcaacttt
     1021 aggacgtaac caataaagat ctcttccact ataaaatggt gaatcctgta caaacgattc
     1081 cataacagaa cctcctgctg ctacagttaa cacagctgct ggcatggact aataccatct
     1141 tttaatttaa ccctggcgga gacgccatta tttttggacg ttcttgaaac aaaattaatt
     1201 cctgaagaat ctttctccag tttattcagc tcaagattca gtccataatt aacgtcaaat
     1261 aactctgata aaggtatcag tttatgttca ggataatgat tatcccgacg acaacaaata
     1321 ttaaaatcct gcatattacc cgacatcagg agttcaaaac cgtccccggc atcattcata
     1381 ctggcagata aattcattac ccagtttagt gctaatcaac aggtacggct taacttacag
     1441 gaattccccg ggaggggcgg actcatcctc agttagcggc aaaatcgcat cacagccaat
     1501 cgcctgtctc gattccccat cggcgaacaa tgtatgtggc cacaaaatcc tggctggctc
     1561 agaacgaaga gcatctgagc aaagaggtca tcaatctggt atgaatatct ggcagaaaat
     1621 atcccctgaa tccacaactg ctgtgcatat ctgctcaaat cagcatcaat cataaaccag
     1681 taatcacagt gcctgtgatt gggttacagg aataagtccc ccttcataag gcatatcatc
     1741 aataccggtg aaggaaaaga aagcatcaga tggcctgata gtctgcgtca gtcaatgaca
     1801 tcagtgcttt atccggaaca atgtatccat gttggcgaca gagaaagcca ttgccatgaa
     1861 cagttttgtt tatccccgga aagtcatttc tgttacaaat ttatattgac aaacttgctt
     1921 tcgatgagaa acatacta
//
LOCUS       DBFIFI010000376         1935 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.378, whole genome shotgun sequence.
ACCESSION   DBFIFI010000376 DBFIFI010000000
VERSION     DBFIFI010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.378"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..572)
                     /locus_tag="N7B39_004896"
     CDS             complement(<1..572)
                     /locus_tag="N7B39_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HGU2424697.1"
                     /translation="MSKIFICAAIPDEQAIKEDSAVAVATAIEAGDERRARAKFHWQF
                     LEQFPAAQDCAYKFIVCEDKPGIPRPALDSWDTEYMQENRWDEESASFVPVEPESDPM
                     NVNFDKLSPEVQNAVLVKFDTCENITVDMVISAQELLQEDMATFGGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCEPGAKWPEIQA"
     gene            complement(668..856)
                     /locus_tag="N7B39_004897"
     CDS             complement(668..856)
                     /locus_tag="N7B39_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HGU2424698.1"
                     /translation="MTSAFALVMTVFLITGESQNVITGIYASKESCLQARDEQKISGE
                     CLPVKKVSLYLNNETPAG"
     gene            complement(853..1041)
                     /gene="dicB"
                     /locus_tag="N7B39_004898"
     CDS             complement(853..1041)
                     /gene="dicB"
                     /locus_tag="N7B39_004898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HGU2424699.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNKAFTEDAINMRKYEPYLLNDN
                     SILSRIALIKLGIFGERQ"
     gene            complement(1522..1674)
                     /locus_tag="N7B39_004899"
     CDS             complement(1522..1674)
                     /locus_tag="N7B39_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HGU2424700.1"
                     /translation="MDMLNLGNNESLVCGVFPNHDGTFTAMTYTRSKTFKTEAGAHRW
                     LARNAN"
BASE COUNT          490 a          459 c          439 g          547 t
ORIGIN      
        1 tctgcctgaa tttcaggcca tttagcgcca ggctcacatt tatgcttaac ccacccgatg
       61 gcatgcagtt taagttcagg atacatggcg ttaacttctg gcattttcat caacgcttca
      121 acgatatgtc cgccgaatgt tgccatgtct tcctgtagta attcctgtgc gctaatcacc
      181 atatcaacgg tgatgttttc gcatgtgtcg aatttaacca ggactgcgtt ctgtacttca
      241 ggggacagct tgtcaaaatt gacgttcatc ggatcggatt ctggttcgac cggtacaaag
      301 gaagctgact cctcatccca gcggttttcc tgcatatatt cggtatccca ggaatcgagg
      361 gcagggcggg gtatgccggg tttatcctcg cagacaataa atttataagc gcagtcctga
      421 gctgccggga attgctccag aaattgccag tgaaattttg cgcgtgcgcg acgctcatca
      481 ccggcttcaa tggcagtggc caccgcaaca gcgctatctt cttttatggc ctgttcgtca
      541 ggaatagcag cgcaaataaa gattttactc attgtgtttt aacctcatta cagatttcag
      601 ggtgaacgaa tccctgccat tgctggcatt tttaatccgt tggtatggcg ttaatatggc
      661 tggcgggtta tccagccggt gtttcgttat tcaggtacag cgatactttt tttaccggta
      721 ggcattcacc agaaattttt tgctcgtctc ttgcctggag gcaggattct ttactggcat
      781 aaattccggt aataacattc tgtgattcac ccgttataag aaaaaccgtc atcaccagtg
      841 caaatgctga agtcattgac gttctccgaa aataccaagt ttaataaggg caattcggga
      901 aagaatggaa ttatcattga gcagataagg ctcatatttc cgcatattaa tggcatcctc
      961 agtaaacgct ttattactga gcagaacacc aatatcaaaa caaccttcag acgtattaac
     1021 gtttggtaat aacgtttcca ttatcgcgtc ctcaacaatg aattttgtga tgcagtgcct
     1081 ggtgcctcca ggtgacgtta accagttaac aattaacgtc gggttagttg atgctcgtta
     1141 cgccagtaaa agaccgcctt actgctttaa ctgttccgcg tgcgcatagc cgcattcacc
     1201 gcatcacaaa attcacttta aaaagggcgg atatccattt ccgccgaatc accagaaaag
     1261 tgataacaga ggttgttgtg gctggggtgt cacttaagcg tatggtcaac ctgacaaccc
     1321 ggtgtcctca acgggggaag gaataacccc gccatactta ccgccgcgcc atttcgcgga
     1381 ttgccacaac cagaagcgca cggtcgaatt aaatttaacg acgacctata cagagagact
     1441 aacttcgccg tgcgctttcg tgttatgccc tgacttttca gggtaaatta acctgtggaa
     1501 acctgttttt actggcgcta atcagttagc gtttcttgct aaccagcgat gcgcgccagc
     1561 ttcggtttta aacgttttac ttctggtata cgtcatcgcg gtaaacgtgc cgtcgtggtt
     1621 ggggaatact ccgcacacca gagattcatt gttgccaaga ttgagcatat ccatgttgac
     1681 ctcatttacc cttaacgccg ggtcgcggaa ctaaaaacct gctgcgctgt tatacaaagt
     1741 gttcccgccg tcatgttcat acgcctcggg ctggctactt aacccctgac cactgccggg
     1801 taactcgagg tattgcccga cgttctgtgg ggcgaggtag ggtggtatgt aataaatcta
     1861 aaagtattta gtttttcagt caaggtaaat ctagattaat ttaaaaaaaa ggtcggcatt
     1921 gccgacctca agtaa
//
LOCUS       DBFIFI010000377         1902 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.379, whole genome shotgun sequence.
ACCESSION   DBFIFI010000377 DBFIFI010000000
VERSION     DBFIFI010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.379"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..275
                     /gene="perB"
                     /locus_tag="N7B39_004900"
     CDS             <1..275
                     /gene="perB"
                     /locus_tag="N7B39_004900"
                     /EC_number="2.3.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-perosamine N-acetyltransferase"
                     /protein_id="HGU2424701.1"
                     /translation="NIYSYVAHDCQIGDYVTFAPGAKCNGYVVIEDNAYIGSGAVIKQ
                     GVPNRPLIIGAGAIIGMGAVVTKSVPAGITVCGNPAREMKRSPTSI"
     gene            476..1882
                     /gene="gndA"
                     /locus_tag="N7B39_004901"
     CDS             476..1882
                     /gene="gndA"
                     /locus_tag="N7B39_004901"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HGU2424702.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQI
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
BASE COUNT          510 a          432 c          463 g          497 t
ORIGIN      
        1 caaacatata ctcatacgtt gcacatgatt gtcaaatagg agactatgtt acatttgctc
       61 ctggggctaa atgtaatgga tatgttgtta ttgaagacaa tgcatatata ggctcgggtg
      121 cagtaattaa gcagggtgtt cctaatcgcc cacttattat tggcgcggga gccattatag
      181 gtatgggggc tgttgtcact aaaagtgttc ctgccggtat aactgtgtgc ggaaatccag
      241 caagagaaat gaaaagatcg ccaacatcta tttaatggga atgcgaaaac acgttccaaa
      301 tgggactaat gtttaaaata tatataattt cgctaattta ctaaattatg gcttcttttt
      361 aagctatcct ttacttagtt attactgata cagcatgaaa tttataatac tctgatacat
      421 ttttatacgt tattcaagcc gcatatctag cggtaacccc tgacaggagt aaacaatgtc
      481 aaagcaacag atcggcgtag tcggtatggc agtgatgggg cgcaaccttg cgctcaacat
      541 cgaaagccgt ggttataccg tctctatttt caaccgttcc cgtgaaaaga cggaagaagt
      601 gattgccgaa aatccaggca agaaactggt tccttactat acggtgaaag aatttgttga
      661 atctctggaa acgcctcgtc gcatcttgtt aatggtgaaa gcaggtgcag gcacggatgc
      721 tgctattgat tcccttaagc catacctcga taaaggtgac atcatcattg atggtggtaa
      781 taccttcttc caggacacca ttcgtcgtaa ccgtgagctt tctgcagaag gctttaactt
      841 catcggtacc ggtgtttccg gtggtgagga gggcgcacta aaaggtcctt ccattatgcc
      901 tggtgggcag aaagaagcct atgaactagt tgcgccgatc ctgaccaaaa tcgccgcagt
      961 ggctgaagac ggtgagccat gcgttaccta tattggtgcc gatggcgcag gtcactatgt
     1021 gaagatggtt cacaacggta ttgaatacgg cgatatgcag ctgattgctg aagcctattc
     1081 tctgcttaaa ggtggtctga acctcaccaa cgaagaactg gcgcagatct ttaccgagtg
     1141 gaataacggt gaactgagca gctacctgat cgacattacc aaagacatct tcactaaaaa
     1201 agatgaagac ggtaactacc tggttgatgt gatcctggat gaagcggcaa acaaaggtac
     1261 gggcaaatgg accagccaga gcgcactgga tctcggcgaa ccgctgtcgc tgattaccga
     1321 gtctgtgttt gcacgataca tctcttctct gaaagatcag cgcgttgctg cgtctaaagt
     1381 tctctctggc ccacaagcgc agccagctgg cgacaaggct gagttcatcg aaaaagttcg
     1441 ccgtgcactg tatctgggca aaatcgtttc ttacgctcag gggttctctc aactgcgtgc
     1501 ggcgtctgaa gagtacaact gggatctgaa ctacggcgaa atcgcgaaga ttttccgtgc
     1561 tggctgcatc atccgtgcgc agttcctgca gaaaatcacc gatgcttatg ccgaaaatcc
     1621 gcagatcgct aacctgctgc tggctcctta cttcaagcaa attgccgatg actaccagca
     1681 ggcgctgcgc gatgtcgtcg cttatgcggt acagaacggt atcccggttc cgaccttcgc
     1741 cgctgcggtt gcctattatg acagctaccg cgccgctgtt ctgcctgcga acctgatcca
     1801 ggcacagcgt gactatttcg gtgcgcatac ttataagcgc attgataaag aaggtgtgtt
     1861 ccataccgaa tggctggatt aatctgattt aatcaacaat aa
//
LOCUS       DBFIFI010000378         1893 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.380, whole genome shotgun sequence.
ACCESSION   DBFIFI010000378 DBFIFI010000000
VERSION     DBFIFI010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.380"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            38..253
                     /locus_tag="N7B39_004902"
     CDS             38..253
                     /locus_tag="N7B39_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HGU2424703.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVSGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            253..750
                     /gene="rrrD"
                     /locus_tag="N7B39_004903"
     CDS             253..750
                     /gene="rrrD"
                     /locus_tag="N7B39_004903"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HGU2424704.1"
                     /translation="MSPSLRKAVAAAIGGGAAAIASVLITGPSGDDGLEGVSYIPYED
                     IVGVWTVCHGHTGKDIIPGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            747..1214
                     /locus_tag="N7B39_004904"
     CDS             747..1214
                     /locus_tag="N7B39_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HGU2424705.1"
                     /translation="MSRVTAIISALVICIIVCQSWAVNHYRDNAIAYKEQRDKKVSEL
                     KQATATITDMQQRQRAADALDAKYTKELADAKAKNDALRRKLDNGGRVFVKGKCPVPS
                     SDETSSASGMGNDATVELSPVAGRNVLGIRDGIIRDQTALRTLQEYIRTQCLR"
     gene            1202..1354
                     /locus_tag="N7B39_004905"
     CDS             1202..1354
                     /locus_tag="N7B39_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424706.1"
                     /translation="MPSMIAIILLIILHIWLCRQGGAHFWSESWLNISLLMLDIEHLA
                     RGKGLR"
BASE COUNT          474 a          456 c          504 g          459 t
ORIGIN      
        1 ttattgttgt cagcttccgg ataacgggag acggggtatg taccagatgg aaaaaataac
       61 aacaggtgtg tcatacacca cgtcagcggt ggggacggga tactggctac tgcagttgct
      121 ggacaaagtc tccccatccc agtgggtggc aataggcgta tcgggtagct tggtgtttgg
      181 cttgctgacg tatctgacaa acctttattt caagattaaa gaagataagc gtaaggctgc
      241 gagaggtgaa taatgtcgcc atcattacgc aaggctgttg ctgctgctat tggtggtggg
      301 gctgctgcca tagcgtctgt gctcatcact ggtccgagtg gtgacgatgg cctggaaggt
      361 gtcagctaca taccatacga agatatcgtt ggcgtatgga ctgtatgtca cggacacacc
      421 ggaaaagaca tcattcccgg taaaacgtat accgaagcag aatgcaaagc cctcctgaat
      481 aaagaccttg ccacggtcgc cagacaaatt aacccgtaca tcaaagtcga tataccggaa
      541 acaacgcgcg gcgctcttta ctcgttcgtt tacaacgtgg gcgctggtaa tttcagaaca
      601 tcgacgcttc ttcgcaaaat aaaccagggt gatatcaaag gcgcatgtga tcagctacgg
      661 cgctggacat acgctggcgg taagcaatgg aaagggctga tgactcgccg cgagattgag
      721 cgtgaagtct gtttgtgggg gcaacaatga gcagagtaac cgcgattatc tccgctctgg
      781 ttatctgcat catcgtctgc cagtcatggg cggttaatca ttaccgtgat aacgccatcg
      841 cctataaaga acagcgtgat aaaaaagtca gtgagctgaa gcaggcgacc gccaccatta
      901 ctgacatgca gcagcgccag cgtgctgctg atgcactcga tgctaaatac acgaaggagt
      961 tagctgatgc gaaagctaaa aatgatgctc ttcggcgcaa gcttgataat ggtggtcggg
     1021 tgttcgtcaa aggaaaatgc cctgtgccat cctcagacga aacctccagc gcctccggca
     1081 tgggcaatga tgccaccgtc gaactctctc cagttgctgg acgaaacgtt ctcggtatcc
     1141 gggacggaat tatccgcgac caaacagcac tgagaacgct tcaggaatac atcaggacgc
     1201 aatgccttcg atgatagcga taattttact catcatcctt cacatctggc tctgtagaca
     1261 gggtggtgct cacttctgga gtgaatcatg gttaaacatc tcattgctga tgcttgatat
     1321 tgagcatctg gcgcgcggta aggggctgcg ttgagataag agccagtcat taaaaatacc
     1381 tggatttagc ctcgcattcg cggggctttt ttattgccat tacaaaagcc actccctaca
     1441 gagtggcttt gataatggct tataccctac acgggataac ttaactgata tcccttttaa
     1501 cggataaagg tattcaagcc tgacacatca tgcgctgtat cgtcgctgta ttcccgcatt
     1561 aaccatgacc gtagcccgac ggggaactcc ttctgcgcga gtgtgcggga ataatcaaaa
     1621 acgatgcaca ccgggttttt accgcgctaa tgattcgcgg gtttgtccct catgctcgcc
     1681 agtcctgtgc gggggtggaa gaaacaggac actcacacag attcttgtgg gtcgatgcta
     1741 ttcctttctg gattatcccg atgccattca tgcaagggct gtatcagacg ttcgtcatgg
     1801 ctttcaggct gacggctcct cccggtgggg tggcctgcca cggggcggga gcgtcgcgga
     1861 aaatggctag tttttgcatt tttatcggcc acc
//
LOCUS       DBFIFI010000379         1863 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.381, whole genome shotgun sequence.
ACCESSION   DBFIFI010000379 DBFIFI010000000
VERSION     DBFIFI010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.381"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..473
                     /locus_tag="N7B39_004906"
     CDS             <1..473
                     /locus_tag="N7B39_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001486660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU2424707.1"
                     /translation="LPVGVPVPWPSATPPTGWLKCNGAAFSAEEYPELAKVYPTNKLP
                     DLRGEFIRGWDDGRGVDNGRGLLTLQDGAIVSHNHYWGIWTSRTNDQTLESFTGTTIL
                     KQITPLSPAINFDNYPIPNPAITEGGVVAATTKPAGANETRPRNVAFNYIVRAA"
     gene            473..1075
                     /locus_tag="N7B39_004907"
     CDS             473..1075
                     /locus_tag="N7B39_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HGU2424708.1"
                     /translation="MNNAELNSELIATMAGEITVYNFDVMSREYISASTEYLAIGVGI
                     PAYSCLDAPGAYKAGYAICRSADFNSWEYVPDHRGEIVFSTETGESKEITAPGDYPDN
                     TTTIAPLTPYDKWDGEKWVTDTEAQHSAAVEAAETQRQSLIDTAMASISLIQLKLQAG
                     RKLTQAETNRLNTVLDYIDAVTATDTSTAPDVIWPELPEA"
     gene            complement(1047..1490)
                     /locus_tag="N7B39_004908"
     CDS             complement(1047..1490)
                     /locus_tag="N7B39_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HGU2424709.1"
                     /translation="MNEYVYSARHNAFFPVDMIDKYKSEGWDLSDAKEVNQNIISEFM
                     AEPPQGKIRIAGDDGLPAWADIPPPTHEELIEITESERQLLINQANEYMNSKQWPGKA
                     AIGRLKGEELAQYNLWLDYLDALELVDTASAPDIEWPTPPAVQAR"
     gene            complement(1511..>1863)
                     /locus_tag="N7B39_004909"
     CDS             complement(1511..>1863)
                     /locus_tag="N7B39_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU2424710.1"
                     /translation="RDVGTGENQIPDMASFASGDGWMKLPNGKILQYGRGAVTPTLST
                     QTMRITFSIPFPKKADCAMLTHSGDGGAPLGAGRGFVMTAEGPTLTGFNSAYRTSSTS
                     DTVSMNYSWWAVGE"
BASE COUNT          494 a          460 c          428 g          481 t
ORIGIN      
        1 cattacccgt tggtgtgcct gttccgtggc cttcagccac tccgccgaca ggctggctga
       61 aatgcaacgg ggcggctttt tctgctgaag aatacccgga actggcaaag gtttacccga
      121 caaataaatt gcctgattta cgtggtgagt ttattcgtgg ctgggatgac ggacgtggag
      181 tggataacgg aaggggatta ttgacgcttc aggacggtgc gattgtcagt cataaccact
      241 attggggaat ctggacttca cgaactaacg accagactct ggaaagtttt acaggcacca
      301 cgattttaaa acaaatcacg cccctgtctc cggccattaa cttcgataat tacccaattc
      361 ccaacccggc tattacagag ggtggtgttg ttgcggcaac gactaaacct gcaggtgcga
      421 atgaaacacg cccacgaaat gtcgctttta actatattgt gagggctgca taatgaataa
      481 cgcagaatta aacagtgaat taattgccac tatggcagga gaaattactg tttataactt
      541 tgatgtcatg agtcgggaat atatttcagc ttcaactgaa tatcttgcta ttggtgtcgg
      601 cattccggca tattcctgtt tagatgcccc aggcgcatac aaagctggtt atgcaatctg
      661 ccgttctgca gattttaact catgggaata tgtgccagac catcgcggtg aaatcgtctt
      721 tagcaccgaa acaggagaat caaaagaaat caccgctccg ggtgattacc ctgataatac
      781 aaccactatc gccccgttaa cgccatacga taaatgggat ggtgagaaat gggtgacgga
      841 taccgaagca cagcatagcg ccgcagtaga agcggcagaa acacaacgcc agtcgctgat
      901 tgatactgca atggcttcca ttagtctgat tcagctgaaa ttacaggccg gacggaagct
      961 gacgcaggca gaaaccaacc gacttaacac ggtgctggat tacattgacg cggtgacggc
     1021 aacagatacc agcaccgcgc cggatgtcat ctggcctgaa ctgccggagg cgtaggccat
     1081 tcaatatctg gcgcactggc ggtatcgacc agctccagtg cgtccagata atccagccac
     1141 aaattatatt gtgccagttc ctcacctttc agacgaccaa tagcggcttt accgggccat
     1201 tgcttactgt tcatgtattc gttggcctgg ttaattagta gctgtctttc tgattcagta
     1261 atttcaataa gttcttcatg cgtgggtgga ggaatatctg cccacgcagg cagcccatca
     1321 tctccggcaa tacggatttt tccttgtggc ggttcagcca taaactcact gataatattt
     1381 tgatttactt ccttagcgtc tgataaatcc catccctctg atttatattt atcaatcata
     1441 tccacaggga aaaaagcatt atgccttgcg ctataaacat attcgttcat ataaatcacc
     1501 ctgaataaaa ttactcacca acagcccacc aactgtaatt catcgatacc gtgtcgctgg
     1561 ttgatgacgt tctgtaagca gaattaaagc cggttaacgt tgggccttct gcagtcatca
     1621 cgaaccctcg cccagcgcct aaaggcgcac cgccatcacc agaatgagta agcatggcgc
     1681 agtccgcttt tttggggaaa gggatgctga atgtaattct cattgtttgc gtcgataatg
     1741 tcggcgtaac cgcaccacga ccatattgca ggattttacc gttgggtaat ttcatccatc
     1801 catcaccact ggcaaaagag gccatgtccg gtatctgatt ttcccctgtc cccacatccc
     1861 gtt
//
LOCUS       DBFIFI010000380         1831 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.382, whole genome shotgun sequence.
ACCESSION   DBFIFI010000380 DBFIFI010000000
VERSION     DBFIFI010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.382"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..430)
                     /locus_tag="N7B39_004910"
     CDS             complement(<1..430)
                     /locus_tag="N7B39_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HGU2424711.1"
                     /translation="MSIKHYDVVRAASPSDLAEKLTHKLKEGWQPYGGPVAITPYTLM
                     QAVAIEGEPQVGPSSEPDWYYVIVLAGQSNAMAYGEGLPLPDSYDAPDPRIKQLARRS
                     TVTPGGAACRYNDIIPADHCLHDVQDMSTLNHPRADLSKGQ"
     gene            407..549
                     /locus_tag="N7B39_004911"
                     /pseudo
     CDS             407..549
                     /locus_tag="N7B39_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(744..911)
                     /locus_tag="N7B39_004912"
     CDS             complement(744..911)
                     /locus_tag="N7B39_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HGU2424712.1"
                     /translation="MTVKLRLTVAALLLFLVVMVDFTSRIMSVLADGVLVCGIMVLLW
                     PVIKRNSLHNA"
     gene            complement(908..1336)
                     /locus_tag="N7B39_004913"
     CDS             complement(908..1336)
                     /locus_tag="N7B39_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance protein"
                     /protein_id="HGU2424713.1"
                     /translation="MVFLVKKARRAATEIKKFEKRDLAQVVINAAYLVACADGECEAS
                     EKAKIEQVLRNQPALYAFTSEINAISATIIGQLDTNFKTGRRAALREIEDVKHDTREA
                     EDVLDVAVAIAEADGEIEPEERKVLEEIAGVLGLRLENYL"
     gene            complement(1510..1586)
                     /locus_tag="N7B39_004914"
     tRNA            complement(1510..1586)
                     /locus_tag="N7B39_004914"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1550..1552),aa:Arg,seq:tct)
     gene            complement(1600..1676)
                     /locus_tag="N7B39_004915"
     tRNA            complement(1600..1676)
                     /locus_tag="N7B39_004915"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1640..1642),aa:Arg,seq:tcg)
     gene            complement(1684..1759)
                     /locus_tag="N7B39_004916"
     tRNA            complement(1684..1759)
                     /locus_tag="N7B39_004916"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1724..1726),aa:Ile,seq:cat)
BASE COUNT          458 a          520 c          441 g          412 t
ORIGIN      
        1 actgcccttt gctcaggtca gccctcggat gattcagcgt actcatatcc tgcacatcat
       61 gcagacagtg gtcagccgga ataatatcgt tatatctgca ggcagccccg cccggcgtca
      121 ctgtactgcg gcgcgccagc tgtttaatgc gcggatccgg agcatcgtat gaatccggca
      181 gcggaagccc ttcaccgtaa gccatggcat tggactgccc ggccagtacg atgacgtagt
      241 accaatccgg ctcagatgaa gggccgacct gtggctctcc ttcaatagcc accgcctgca
      301 tcagtgtgta cggcgtaatg gcaaccggtc cgccgtatgg ctgccagccc tctttcagtt
      361 tgtgtgtcag cttttccgca aggtctgacg gcgacgccgc cctgacaaca tcataatgtt
      421 taatcgacat cgaatttctc ccgtgtagag gaacagagtt aaaaagccgg aagcggaatc
      481 aaatcacagg atgaccatct gccagtggct ggtcgtaaaa aaaggccgcg ccatgcgcag
      541 ccgaaaataa agggataacg atgatagttt gagaaaaaca gaaataacac ttttgtggca
      601 aagcatggtg ccgggtgcct cccggtgaat tcagtatcag cacctgaatc cgcgattacc
      661 ccatattcct tcttgctgat tgccccaccg cacaggggga ttcaccatgc agaagtgttt
      721 ttaataaaca gcaaacaaaa aaatcaagca ttatgcaggc tgtttctttt tatcaccggc
      781 cacagcaata ccataatgcc gcagaccagc accccatccg ccagcaccga catgattctg
      841 ctggtgaaat ccaccatcac caccagaaac agcaggagtg cagccacagt caggcgcagt
      901 tttaccgtca caggtaattc tccagacgaa gacccagaac accggcaatc tcttccagca
      961 ccttgcgctc ttccggctca atttcgccgt ctgcctccgc aatggccacc gccacatcca
     1021 gcacatcttc cgcttcacgc gtatcgtgtt tcacatcctc gatctcacgt aacgccgcac
     1081 gacgaccagt tttaaagttc gtatccagct gaccgataat ggttgcgcta atcgcattaa
     1141 tttctgacgt aaacgcgtac agcgcaggct gattacgcag tacctgttcg atcttcgctt
     1201 tctcggatgc ctcacattca ccatctgcac aggccaccag gtatgcggcg ttaatcacca
     1261 cctgtgccag atcgcgtttt tcaaactttt taatttccgt tgccgctctg cgggcttttt
     1321 ttaccaaaaa taccaaacat cgtgacgttc ctttgggtgg gtgagccaac gcccgggagc
     1381 gatctgccca cagagaaagt cacactgacc actccataag ctccccccga aaggctctgt
     1441 ggttggtatg cgccgggcgt ggtgcggata caaaaaaggt ccgcaaaagc gagcgaggga
     1501 aaataagtgt ggtgcgttgt actgggttcg aaccagtgac cgattgctta gaaggcaatt
     1561 gctctgtccg gctgagctaa aaacgcagaa taccgataat ggaccgccat cggagactcg
     1621 aaccccgcga aaccagcttc gaaggctggc gttctatccc gatgagctaa tggcggtatg
     1681 tgatggtggc ccttgctgga tttgaaccag cggcctggcg attatgagtc gctcgctctc
     1741 accactgagc taaagggccg ggcgcaggat aataacgtta cgaaatcaat gttgcaagca
     1801 ttcaagaatc acctggttaa aaattaccct t
//
LOCUS       DBFIFI010000381         1811 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.383, whole genome shotgun sequence.
ACCESSION   DBFIFI010000381 DBFIFI010000000
VERSION     DBFIFI010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.383"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..675)
                     /gene="nleH1-2"
                     /locus_tag="N7B39_004917"
     CDS             complement(<1..675)
                     /gene="nleH1-2"
                     /locus_tag="N7B39_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector kinase
                     NleH1-2"
                     /protein_id="HGU2424714.1"
                     /translation="MLSPSSINLGCSWNSLTRNLTSPDNRVLSSVRDAAVHSDSGTQV
                     TVGNRTYRVVVTDNKFCVTRESHSGCFTNLLHRLGWPKGEISRKIEAMLNTSPVSTTI
                     ERGSVHSNRPDLPPVDYAQPELPPADYTQSELPRVSNNKSPVPGNVIGKGGNAVVYED
                     MEDTTKVLKMFTISQSHEEVTSEVRCFNQYYGSGSAEKIYNDNGNVIGIRMNKINGES
                     LLDIPSL"
     gene            complement(782..853)
                     /locus_tag="N7B39_004918"
                     /pseudo
     CDS             complement(782..853)
                     /locus_tag="N7B39_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
     gene            847..1811
                     /locus_tag="N7B39_004919"
                     /pseudo
     CDS             847..1811
                     /locus_tag="N7B39_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          543 a          440 c          375 g          453 t
ORIGIN      
        1 taatgatgga atatccaaaa gagattcccc atttatttta ttcattctaa taccaataac
       61 atttccatta tcattatata ttttctctgc actcccggaa ccataatact gattgaaaca
      121 acgaacttcg cttgtcacct cttcatggct ttgagatata gtaaacatct tcaacacttt
      181 tgttgtatct tccatatctt catacacgac agcattacca cctttaccaa taacattacc
      241 tggcacgggt gatttattgt tgctaaccct cggcaactct gattgagtat aatccgctgg
      301 aggtaactcc ggctgcgcat aatccactgg aggtaaatca ggtctgttcg aatgaacaga
      361 gcctctttct atagtcgtgc tcactggcga tgtattcagc atagcctcaa tttttctgct
      421 aatctctccc ttaggccatc ccaacctgtg caacagatta gtaaaacaac cactatgact
      481 ttctcttgta acgcaaaact tattatcagt gaccacaaca cgatatgttc tgttgccaac
      541 cgttacttgc gtcccgctat cagagtgaac agcagcatcc cttacagagg ataaaacacg
      601 attatcaggc gaagtcaggt ttctggttaa agaattccat gaacatccca aatttataga
      661 agagggcgat aacatacatt tcaaccttca aaataaacct atctaattat tcctaacaga
      721 catcccccat acatgacaac aaaaaccgga gccggactcc ggtttttgtg aagctatcgg
      781 gttacttcat ttcgccaata ttttcccact tcccgtcagc acgcaggatt tgcagcggtc
      841 ttactacaca ctgacccgag gacgaaaaaa gagttcgggc tgggaagaga cagaagagta
      901 gcaatttcag aagcattcac accaacattg agcttttcag cgaaagtggg cacgaatcat
      961 tgctggacag ggttaaaggc actgactcaa tcactcttca tacgtggctt gatcgatatg
     1021 aaacaatcct cagctagagg gggagcaagc cgaaaacatt actcgactac gccagcaaaa
     1081 tcagggcaat tcgaagaaaa ttgccggaca aaccgctcac tgacatatca acgaaagagg
     1141 tggcagcaat gctaaacacc tacgtcgcag aaggtaaagc ggtttccgca agagtaatca
     1201 ggtcaaccct tgttgacgtt tttcgagggg caatagccga ggggcatgtg gcaacgaatc
     1261 cagtaacaac aacccgtgca gcaaagtcag aagtaaggcg ctcaaggctg acagctaatg
     1321 agtatgtcgc tatttaccat gctgctgagc acctccccat ctggctgagg ctgtcaatgg
     1381 atttagctgt cgttacaggg cagagagtgg gcgatttgtg cagaatgaag tggtcagaca
     1441 taaacgatgg tcatcttcac attggacaga gtaaaacagg agccaaaatt gccattccgc
     1501 tggctctaac cattgacgca ctcgacatct cactggttga tacactacag aaatgcaggg
     1561 aggccagcag cagtgaaaca ataatcgcat caacctatca cgaaccactt tctccagcca
     1621 cagtatcacg gtatttaaca aaggcgcgaa atgcatccgg gatctcgttt gatggagacc
     1681 caccgacatt tcatgaacta cgtagtctgt ccgcgaggct atatcggaac cagattggct
     1741 acaagtttgc acaacgtctt cttggacata aatctgattc aatggcggcg cattataggg
     1801 acagtcgcgg g
//
LOCUS       DBFIFI010000382         1760 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.384, whole genome shotgun sequence.
ACCESSION   DBFIFI010000382 DBFIFI010000000
VERSION     DBFIFI010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.384"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            151..345
                     /locus_tag="N7B39_004920"
     CDS             151..345
                     /locus_tag="N7B39_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424715.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            353..>1760
                     /gene="pykF"
                     /locus_tag="N7B39_004921"
     CDS             353..>1760
                     /gene="pykF"
                     /locus_tag="N7B39_004921"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HGU2424716.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHV"
BASE COUNT          474 a          438 c          417 g          431 t
ORIGIN      
        1 tgcgtaacct tttccctgga acgttaaatc tttgataaca atttattgtc taacaagttg
       61 tatatttttt gaaacgctgt ttttgttttc cttttggatt aatttcagcg tataatgcgc
      121 gccaattgac tcttgaatgg tttcagcact ttggactgta gaactcaacg actcaaaaac
      181 aggcactcac gttgggctga gacacaagca cacattcctc tgcacgcttt ttcgatgtca
      241 cctatcctta gagcgaggca ccaccacttt cgtaataccg gattcgcttt ccggcagtgc
      301 gcccagaaag caagtttctc ccatccttct caacttaaag actaagactg tcatgaaaaa
      361 gaccaaaatt gtttgcacca tcggaccgaa aaccgaatct gaagagatgt tagctaaaat
      421 gctggacgct ggcatgaacg ttatgcgtct gaacttctct catggtgact atgcagaaca
      481 cggtcagcgc attcagaatc tgcgcaacgt gatgagcaaa actggtaaaa ccgccgctat
      541 cctgcttgat accaaaggtc cggaaatccg caccatgaaa ctggaaggcg gtaacgacgt
      601 ttctctgaaa gctggtcaga cctttacttt cactactgat aaatctgtta tcggcaacag
      661 cgaaatggtt gcggtaacgt atgaaggttt cacaactgac ctgtctgttg gcaacaccgt
      721 actggttgac gatggtctga tcggtatgga agttactgcc attgaaggta acaaagttat
      781 ctgtaaagtg ctgaacaacg gcgacctggg tgaaaacaaa ggtgtgaacc tgcctggcgt
      841 ttccattgcc ctgccagcac tggctgaaaa agacaaacag gacctgatct ttggttgcga
      901 acaaggcgta gactttgtcg ctgcttcctt tattcgtaag cgttctgacg ttatcgaaat
      961 ccgtgagcac ctgaaagcgc acggcggcga aaacatccac atcatctcca aaatcgaaaa
     1021 ccaggaaggc ctcaacaact tcgacgaaat cctcgaagcc tctgacggca tcatggttgc
     1081 gcgtggcgac ctgggtgtag aaatcccggt agaagaagtt atcttcgccc agaagatgat
     1141 gatcgaaaaa tgtatccgtg cacgtaaagt cgttatcact gcgacccaga tgctggattc
     1201 catgatcaaa aacccacgcc cgactcgcgc agaagccggt gacgttgcaa acgccatcct
     1261 cgacggtact gacgcagtga tgctgtctgg tgaatccgca aaaggtaaat acccgctgga
     1321 agcggtttct atcatggcga ccatctgcga acgtaccgac cgcgtgatga acagccgtct
     1381 cgagttcaac aatgacaacc gtaaactgcg cattaccgaa gcggtatgcc gtggtgccgt
     1441 tgaaactgct gaaaaactgg atgctccgct gattgtggtt gctacccagg gcggtaaatc
     1501 tgctcgcgca gtacgtaaat acttcccgga tgccaccatc ctggcactga ccaccaacga
     1561 aaaaacggct catcagttgg tactgagcaa aggcgttgtg ccgcagctgg ttaaagagat
     1621 cacttctact gatgatttct accgtctggg taaagaactg gctctgcaga gcggtctggc
     1681 acacaaaggt gacgttgtag ttatggtttc tggtgcactg gtaccgagcg gcactactaa
     1741 caccgcatct gttcacgtcc
//
LOCUS       DBFIFI010000383         1758 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.385, whole genome shotgun sequence.
ACCESSION   DBFIFI010000383 DBFIFI010000000
VERSION     DBFIFI010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.385"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..658)
                     /locus_tag="N7B39_004922"
     CDS             complement(<1..658)
                     /locus_tag="N7B39_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase family protein"
                     /protein_id="HGU2424717.1"
                     /translation="MNKQRIFIAGHQGMVGSAITRRLKQRDDVELVLRTRDELNLLDS
                     SAVLDFFSSQKIDQVYLAAAKVGGILANSSYPADFIYENIMIEANVIHAAHKNNVNKL
                     LFLGSSCIYPKLAHQPIMEDELLQGKLEPTNEPYAIAKIAGIKLCESYNRQFGRDYRS
                     VMPTNLYGPNDNFHPSNSHVIPALLRRFHDAVENNSPNVVVWGSGTPKREFLHVDDMA
                     S"
     gene            complement(661..>1758)
                     /gene="gmd"
                     /locus_tag="N7B39_004923"
     CDS             complement(661..>1758)
                     /gene="gmd"
                     /locus_tag="N7B39_004923"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HGU2424718.1"
                     /translation="TGVTGQDGSYLAEFLLDKGYEVHGIKRRASSFNTERIDHIYQDP
                     HGSNPNFHLHYGDLTDSSNLTRILKEVQPDEVYNLAAMSHVAVSFESPEYTADVDAIG
                     TLRLLEAIRFLGLENKTRFYQASTSELYGLVQEIPQKESTPFYPRSPYAVAKLYAYWI
                     TVNYRESYGIYACNGILFNHESPRRGETFVTRKITRGLANIAQGLESCLYLGNMDSLR
                     DWGHAKDYVRMQWLMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKMSFVGKGIEE
                     KGIVDSVEGQDAPGVKPGDVIVAVDPRYFRPAEVDTLLGDPSKANLKLGWRPEITLAE
                     MISEMVAKDLEAAKKHSLLKSHGFSVSLALE"
BASE COUNT          546 a          376 c          316 g          520 t
ORIGIN      
        1 cagaagccat atcatctaca tgtaagaatt cacgctttgg agtaccactt ccccaaacaa
       61 caacattcgg agaattgttt tccacagcat catgaaagcg gcgcaaaagc gccggaatca
      121 catgagaatt acttggatga aaattgtcat ttggaccata aagattggtt ggcattactg
      181 aacggtaatc acgcccaaac tgacggttat aagattcaca taatttaata cctgcaattt
      241 ttgcgatagc ataaggttca tttgttggct caagtttccc ttgtaataat tcgtcttcca
      301 taatcggttg gtgtgctaac ttaggataaa tacacgacga accgaggaaa agcagtttat
      361 ttacattatt tttgtgggca gcatgaatga cattcgcctc tatcattata ttctcatata
      421 taaaatcggc aggataagaa ctgttagcta aaatacctcc gacttttgct gctgccaaat
      481 aaacctggtc gattttctgt gaagaaaaaa aatccaaaac agcgctacta tccaacaagt
      541 tcaattcatc ccgagtacgt aaaaccaact caacatcatc acgttgtttg aggcgtcggg
      601 taatagctga tccaaccatt ccttggtgac cagcaataaa aatacgttgc ttattcatca
      661 tcattccaga gctaagctta cagaaaaacc atgcgatttt aaaagagaat gttttttagc
      721 ggcttcaaga tctttggcaa ccatttcaga aatcatttca gcaagagtaa tttctggtct
      781 ccaaccaagt ttgagattag ctttgctcgg atctccaagc aaagtatcaa cttcagctgg
      841 tcggaaataa cgaggatcaa cagcaacaat gacatcacct ggtttcacac ctggagcatc
      901 ctgtccttca accgaatcta caatgccttt ttcttcgatt cctttaccaa caaagctcat
      961 cttaatacca agttgtgctg ctgccatttc gacaaactga cggactgagt attggactcc
     1021 tgttgcaatc acaaaatctt cgggttgctc ctgttgtaac atcaaccatt gcattctaac
     1081 ataatctttt gcatgtcccc aatctcgtaa cgaatccata ttccctaaat acaaacatga
     1141 ttccaagcct tgtgcaatat ttgcaagtcc tcgagtaatt ttccttgtta caaacgtttc
     1201 tccacggcgt ggagattcat gattgaacaa tataccatta catgcataaa taccatatga
     1261 ctctcgataa tttaccgtga tccaatatgc gtaaagtttt gcaactgcat aaggggaacg
     1321 aggataaaaa ggggtggatt ctttttgagg gatttcctga acaagtccat ataattctga
     1381 ggttgaagct tgatagaaac gcgttttgtt ttccaatcct aaaaagcgaa ttgcttccag
     1441 taaacgtaat gtaccaattg catcgacatc ggctgtatat tctggagact caaaagaaac
     1501 tgctacgtga ctcatagcag ctaaattata tacttcatct ggctgtacct cctttagaat
     1561 tctagtgagg ttagatgaat cagtcagatc tccatagtgc aagtgaaaat ttgggttaga
     1621 accatgtgga tcttgataaa tatggtctat gcgttctgta ttaaaagatg aggctcggcg
     1681 tttgatacca tgaacttcat accctttatc aagcaaaaac tcagctagat aagatccatc
     1741 ttgtccagtt acacctgt
//
LOCUS       DBFIFI010000384         1751 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.386, whole genome shotgun sequence.
ACCESSION   DBFIFI010000384 DBFIFI010000000
VERSION     DBFIFI010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.386"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(331..903)
                     /gene="hdeD"
                     /locus_tag="N7B39_004924"
     CDS             complement(331..903)
                     /gene="hdeD"
                     /locus_tag="N7B39_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HGU2424719.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            1158..1490
                     /gene="hdeA"
                     /locus_tag="N7B39_004925"
     CDS             1158..1490
                     /gene="hdeA"
                     /locus_tag="N7B39_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HGU2424720.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            1606..>1751
                     /gene="hdeB"
                     /locus_tag="N7B39_004926"
     CDS             1606..>1751
                     /gene="hdeB"
                     /locus_tag="N7B39_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HGU2424721.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKA"
BASE COUNT          583 a          378 c          346 g          444 t
ORIGIN      
        1 aatcaacgcc agttgcaaaa aaatgaaccc ggattactta tttaaccgcc catagcatca
       61 acgcttacgg aactctttgt tcactttccc acagcacttt taaaacatta aacctgacta
      121 cggaaaatat cagccatgat aatgtttgaa atggctaatt tgccatggag tgaaaaaaat
      181 tagatgaaat tcagtaggtt gaaataatca ctagcaggta attatttcaa tgatagtgcg
      241 caattgatct acaacactgc gtagcggaga gagtattaat cggatcatag tcacatcaag
      301 tgactatgat ccgggtgaca accgggataa ttattgctgc ttaacgaaca aactggcgaa
      361 gctgaacagg ctggcggcgc taaatatcag ttcaattccc accagtgtgg aaaccagcgt
      421 tacagacacc atcggcgttg caccaaggaa tatccaggca atgacgatat ccagcacacc
      481 aataacgagc tgtagccagc tgcctttcat tgaacgctga cgataccaac tcatcaggcg
      541 aataacccct gcaacacaga acaaaccggc aataaatgcc gcaatggcaa aaatgcccag
      601 ctccggtgcg cggatgaaga aatagccgat caataaatag gcgactgcga cgaggaaacc
      661 ggataatacc ggccagaaat tatgactgcg gttgctgaat aatccaacaa taagcgcaat
      721 gcccgaacag attaataatg cacctactac tgtgcttaaa atatcgccag aaacgaacgg
      781 gaaactgata cacagcaacc cgacgataaa cagcagcacg gcaataaact ggattgctct
      841 gcgatgcttt ttcagcatct ccagatcaaa cttcaaaatt gttgccttat ctatatataa
      901 catagaacca ccctataaaa ttaagaagaa aatcccctgc tatcaatcta tgccaaaaac
      961 gcgtctaaga atgcagtcga tttaataaaa atttcctaat tgcagtatct gatgcatctg
     1021 taactcattg tattgaaata aaaatatctg attttgatat tttccatcaa catgacatat
     1081 acagaaaacc aggttataac ctcagtgtcg aaattgattc gtgacggctc tttcacttta
     1141 tagttgagga tattacgatg aaaaaagtat taggcgttat tcttggtggt ctgcttcttc
     1201 tgccagttgt gagcaatgca gcggatgcgc aaaaagcagc tgataacaaa aaaccggtca
     1261 actcctggac ctgtgaagat ttcctggctg tggacgaatc cttccagcca actgcagttg
     1321 gttttgctga agcgctgaac aacaaagata aaccagaaga tgcggtttta gatgttcagg
     1381 gtattgcaac cgtaacccca gctatcgttc aggcttgtac tcaggataaa caagccaact
     1441 ttaaagataa agttaaaggc gaatgggaca aaattaagaa agatatgtaa ttccgggaat
     1501 gcgttacatc gtacttcctt gcatattgaa caggccggaa tatcttcttt aaaagcagct
     1561 attcctcctg ttcatatata atctctatat tgaatgggtt acaaaataaa tatttcatct
     1621 ctccgtaaag cgtttatttt tatgggcgct gtagcggctt tgtcactggt gaacgcacaa
     1681 tctgcgctgg cagccaatga atccgctaaa gatatgacct gccaggaatt tattgatctg
     1741 aatccaaaag c
//
LOCUS       DBFIFI010000385         1717 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.387, whole genome shotgun sequence.
ACCESSION   DBFIFI010000385 DBFIFI010000000
VERSION     DBFIFI010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.387"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..81
                     /locus_tag="N7B39_004927"
     CDS             <1..81
                     /locus_tag="N7B39_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424722.1"
                     /translation="KENTETVEMNSKVWGRLKKIITFFKK"
     gene            complement(157..459)
                     /gene="bamE"
                     /locus_tag="N7B39_004928"
     CDS             complement(157..459)
                     /gene="bamE"
                     /locus_tag="N7B39_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HGU2424723.1"
                     /translation="MKNETQESVKTKIVKGKTTKQDVLASFGEPDSRSLIDGEEQWSY
                     TMYNSQSKATSFIPVVGLLAGGADSQTKSLTVSFKGEKVSTYIFNAGTSNVKTGIF"
     gene            complement(510..1250)
                     /locus_tag="N7B39_004929"
                     /pseudo
     CDS             complement(510..1250)
                     /locus_tag="N7B39_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415922.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS30 family transposase"
     gene            1312..1717
                     /locus_tag="N7B39_004930"
                     /pseudo
     CDS             1312..1717
                     /locus_tag="N7B39_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          439 a          395 c          389 g          494 t
ORIGIN      
        1 aaggaaaata ctgagactgt agaaatgaac agtaaagttt gggggaggct gaaaaagata
       61 atcactttct ttaaaaagtg atttatgttt ctgtaaaaag cccgtaggta tcaactgcgg
      121 gctaaatttt cccttgatgc tggttttgtc ttttatctaa aaaatgccag tcttcacgtt
      181 gcttgttcca gcattaaaga tgtatgtgct gactttttcg cctttgaaag aaactgtcag
      241 agatttagtt tgtgagtctg cgccacctgc aagcagtccc acaacgggga tgaaagaggt
      301 tgctttggac tggctgttat acatagtgta tgaccattgt tcttcaccat cgatcaaaga
      361 acggctgtca ggttcaccga acgatgctaa cacgtcctgt tttgtagttt tgcctttaac
      421 aattttggtt ttcacacttt cctgcgtctc atttttcaat gactggttgc ctactgaaga
      481 acaacctgcc aaggtcagaa aaacgaatct atatgagagt tttttgtacc tgagactaaa
      541 tcgccctccc aatgccctag agagcgtctg ttatcgatat ttcgggaacg ttcgtgaatt
      601 ggtgttccgt tcactatgtt aatcgtacct ctttcgcctt tgcgggtatg acgcctgcca
      661 tggcgaaggc tatgcgaccg tcgcagatgc tgtatattca ggtggtgtag cgcttcacgg
      721 ctacgaaagt acagcgtttt ataaattgtc tcaggtgata ttcgcagcgt tttttgacgt
      781 ggttttgttc gccttaacca tcctgatatt tgctctggag accatttcat ctccagcttt
      841 tccagaacaa gctttcgcaa tggtaaattt tgatccagta agcacggttt tggccttttc
      901 gccattctgt tggctcggtt attagcatca acagctttgt aatagcgtct gccccgatta
      961 cgctgaactt cacgtgagat cgtcgaagga ctgcgattca gcgcagtagc tatcgcacga
     1021 atgctcattt tggctgacaa accagctcgt atctcctcgc gctcagacag tgtcaggtga
     1081 gctacagccc gcttacgctc atggggtttt atgccgccag tatcccttaa catagtgaag
     1141 atcgttccgg gttttgaacc caggatattc gctatttcac tgaagcctgt tccgttcttc
     1201 catagttcaa aaacagaggc tttttcctct gctgtaaatg ttcgtctcat tcaaaaaacc
     1261 tccgcaaccc catgttttca cataactgtt gcgttgacca attgaatcta caacagcgta
     1321 ttttgcacag gagtcgctga aaaatacgcg ttaatcgaac aatggcgaca acaatttccc
     1381 attgaagcga tgtgtcaggt atttggtgta tccaggagcg gttattacaa ctgggtacag
     1441 catgaaccct cagacagaaa acaaagtgat gagcggctaa aactggagat taaggtggca
     1501 catatccgca ctcgcgaaac atatggaacc cggcggctcc agacggagct ggcagagaat
     1561 ggcatcatcg ttggtcgtga ccgactggca cgtcttcgta aggagctaag gctacgctgt
     1621 aagcagaaac gcaagttcag ggcgactacg aactcgaacc acaatctgcc agttgcgcca
     1681 aatctgctga accagacgtt cgctcctaca gcaccaa
//
LOCUS       DBFIFI010000386         1716 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.388, whole genome shotgun sequence.
ACCESSION   DBFIFI010000386 DBFIFI010000000
VERSION     DBFIFI010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.388"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            161..1342
                     /locus_tag="N7B39_004931"
     CDS             161..1342
                     /locus_tag="N7B39_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU2424724.1"
                     /translation="MPLNDMQIRRAKPEAKAYTFGDGLGLSLLIEPNGSKSWRFRYRY
                     AGKPKMISLGVYPTITLADARSRRDEARKLVAEGKNPSEVRKEQKLAMQTESENAFEK
                     IAREWHQLKSAKWSAGYASDIMEAFKNDIFPYVGTRPVGEIKPLELLNVLRKIEKRGA
                     LEKMRKVRQRCSEVNRPAIATGRAEYNPAADLSSALEVHQSNHFPFLKADEIPDFLRA
                     LEGYSGSKLVQIATKLLMITGVRTIELRAALWQEFDLDNAIWEIPAERMKMRRPHLVP
                     LSSQAVDLLNELKIMTGNYRYVFPGRNDPNRPMSEASINQAIKRIGYGGKVTGHGFRH
                     TLSTILHEQGFESAWIEIQLAHVDKNSIRGTYNHAQYFSGRKSMMDWYSNLIFERLKR
                     S"
     gene            1346..>1716
                     /locus_tag="N7B39_004932"
     CDS             1346..>1716
                     /locus_tag="N7B39_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424725.1"
                     /translation="MSQPLSEILTWDDEQWEVFVHDWLIVCKSDDYPWSERLGGAGDK
                     GRDVVGYKSDPNVEGYSWDNYQCKLYKKSLGFSDVVVEFGKLIYFTLNGDYPIPQKYF
                     FVAPYDLSTTFSNLLKNKNEL"
BASE COUNT          503 a          325 c          404 g          484 t
ORIGIN      
        1 tcaacttcct caaacgtccg tattagtcca taaaatctct gatttataac attttttatt
       61 cttttaagtc catagtagtc cgtaaccatc cagtagaatc cggtactgaa tgtgtatagg
      121 attgtgtata tgttcctgtt cggtctggat tcctatacac atgcctttaa acgatatgca
      181 gattcgccgc gctaagcctg aagctaaagc ctatacattt ggagatgggc tagggttgtc
      241 attacttata gaacctaatg gaagcaagag ttggcggttc cgctatcgct atgccggcaa
      301 acccaaaatg atctcgcttg gtgtttaccc aacgatcacc cttgccgatg ctcgttcccg
      361 tcgtgatgaa gctcgaaaac ttgtggcaga aggaaagaac cctagtgagg ttcgaaaaga
      421 gcaaaagcta gctatgcaaa cagagtcaga gaacgccttc gaaaagatag ccagagagtg
      481 gcatcaactt aaatctgcta aatggtcggc gggatatgca tcagacatca tggaagcgtt
      541 taagaacgac atttttcctt atgtcggaac aaggcctgtg ggagagatta aaccgctaga
      601 gctgctgaac gttctgcgta aaattgagaa acgtggtgcg ttggagaaaa tgcgcaaagt
      661 gcggcagcgt tgctccgaag tgaaccgccc cgcaattgca acgggtaggg cggagtacaa
      721 tcctgcggct gatctctcca gcgctctcga agtacaccaa tccaatcatt tcccattcct
      781 aaaagctgat gagatacctg attttctacg tgccttagag ggttactccg ggagtaagct
      841 tgtccagata gccacgaaat tactgatgat tacgggtgtg agaaccatcg aattacgcgc
      901 ggcattatgg caagaatttg atctggataa cgctatttgg gaaattcctg ctgaaaggat
      961 gaaaatgcgt aggccacatc ttgtgccctt atcatctcaa gcggtagatt tactcaatga
     1021 actcaagatc atgacaggga actatcgtta tgtttttcca gggcggaacg atccgaatag
     1081 gccaatgagc gaagcgagta taaatcaagc cattaagcgt attgggtatg gaggaaaagt
     1141 cactggacat ggttttcgtc ataccctttc tacaatcctg catgagcaag gttttgagag
     1201 tgcttggatt gaaatccagt tggctcatgt agataaaaat tctattaggg ggacttataa
     1261 ccatgctcaa tattttagtg gaaggaagtc tatgatggac tggtacagta atttgatatt
     1321 tgaaagacta aaaaggagtt aatttgtgtc tcaaccactt tcggaaatat tgacatggga
     1381 tgatgaacaa tgggaggtat ttgtccatga ttggcttatt gtctgtaaat cagatgatta
     1441 cccgtggagc gaacgtttgg gaggagctgg agataaaggt agagacgttg ttggatataa
     1501 atcggatcct aacgtagaag gttattcttg ggataattat caatgcaaac tgtacaaaaa
     1561 aagtttaggg ttctctgatg ttgtagttga gtttggaaaa cttatctatt ttactctgaa
     1621 tggtgattat cccatccctc agaagtactt ttttgtggca ccctatgatt tatctactac
     1681 attttctaat ttattgaaaa ataaaaacga gcttaa
//
LOCUS       DBFIFI010000387         1704 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.389, whole genome shotgun sequence.
ACCESSION   DBFIFI010000387 DBFIFI010000000
VERSION     DBFIFI010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.389"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..>1704
                     /locus_tag="N7B39_004933"
     CDS             <1..>1704
                     /locus_tag="N7B39_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HGU2424726.1"
                     /translation="HRETARSFGGAGSTAGYEQVTAYTLTGQLQSRHLNLPQLDCDYD
                     WNDNGQLIRISGPQESREYRYSDTGRLTGVHTTAANLDIDIPYATDPAGNRLPDPELH
                     PDSTLTAWPDNRIAEDAHYVYRYDEYGRLTEKTDRIPEGVIRMHDERTHHYHYDNQHR
                     LVFYTRIQYGEPLVESRYLYDPLGRRTGKRVWRRERDLTGWMSLSRKPEVTWYGWDGD
                     RLTTVQTGTTRIQTVYRPGSFTPLIRIETENGEREKAQRRSLAEKLQQEGSEDGHGVV
                     FPAELVRMLDRLEEEIRADRVSSESRAWLAQCGLTVEQLEKQVEPEYTPARTLHLYHC
                     DHRGLPLALISEDGNTAWSAEYDEWGNQLNEENPHHLHQPYRLPGQQHDEESGLYYNR
                     HRYYDPLQGRYITPDPIGLRGGWNMYQYPLNPIQVIDPMGLDAIENMTSGGLIYAVSG
                     VPGLIVANSITNSAYQFGYDMDAIVGGAHNGAADAMRYCYLMCRMTKTFGSTIADVIG
                     KNHEAAGDRQGQPAKERIMDLKNNTVGIACGDFSAKCSDACIEKYNIGQLFGLDGIKA
                     D"
BASE COUNT          429 a          429 c          546 g          300 t
ORIGIN      
        1 tgcaccgtga gacggcccgc agcttcggcg gggcgggcag tacagcgggg tacgaacagg
       61 tcacggcgta caccctcaca gggcagctac agagcaggca cctgaacctc ccgcagcttg
      121 actgtgacta cgactggaac gacaacggac agctaatccg catcagcggc ccgcaggaga
      181 gccgggagta ccgttacagc gacacgggaa ggctgacggg cgtccacacc acggcagcga
      241 acctggatat cgatatcccg tatgcaacgg acccggcagg aaaccggctg ccggacccgg
      301 aactgcatcc ggacagcacg ctcacggcgt ggccggataa ccgcatcgcg gaagatgcgc
      361 actatgtcta ccgctacgat gaatacggca ggctgacgga gaagacggac cgcattccgg
      421 aaggggttat ccggatgcac gacgagcgga cccaccacta ccattacgac aaccagcacc
      481 gcctggtgtt ctacacgcgg atacaatacg gcgagccgct ggtcgagagc cgctacctct
      541 atgacccgct gggccgccgg acggggaaac gggtgtggcg gcgggagcgt gacctgacgg
      601 ggtggatgtc gctgtcgcgt aaaccggagg tgacctggta cgggtgggac ggcgacaggc
      661 tgacgacggt acagaccggc accacacgta tccagacggt ataccggccg gggagcttca
      721 caccgctcat ccgcatcgaa acggagaacg gcgagcggga gaaagcgcag cgccgcagcc
      781 tggcggagaa actccagcag gaagggagtg aggacggtca cggtgtggtg ttcccggcag
      841 aactggtgag gatgctggac aggctggagg aagaaatccg ggcagaccgc gtgagcagtg
      901 aaagccgggc gtggcttgcg cagtgcggac tgacggtgga gcaactggaa aaacaggtgg
      961 agccggaata cacgccggcg cgcacgctgc atctgtacca ctgtgaccac cggggactgc
     1021 cgctggcgct tatcagcgaa gacggcaata cggcgtggag cgcggaatat gatgaatggg
     1081 gcaaccagct taatgaggag aacccgcatc acctgcacca gccgtaccgt ctgccgggcc
     1141 agcagcatga tgaggagtcg gggctgtact ataaccgtca ccggtactac gatccgttgc
     1201 aggggcggta tatcaccccg gacccgattg ggttgagagg tggatggaat atgtatcagt
     1261 atccgttgaa tcccatacaa gtgatagacc caatggggtt agatgcgatt gagaatatga
     1321 catcaggtgg actaatttat gccgtatctg gtgtacctgg attgattgtt gcaaacagca
     1381 ttactaacag tgcttaccag ttcggttatg atatggatgc tattgttggc ggagctcata
     1441 atggggccgc cgatgcaatg agatattgtt acttgatgtg tcgaatgact aagacatttg
     1501 gatcaacaat agctgacgtg ataggtaaaa atcatgaggc ggctggggat agacaaggtc
     1561 agccagctaa agaaagaatc atggatctta aaaataacac tgtcggtatt gcttgtggcg
     1621 atttttctgc caaatgtagc gatgcatgta ttgaaaaata taacattggg caactcttcg
     1681 ggttagatgg tataaaagca gata
//
LOCUS       DBFIFI010000388         1700 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.390, whole genome shotgun sequence.
ACCESSION   DBFIFI010000388 DBFIFI010000000
VERSION     DBFIFI010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.390"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            69..1211
                     /locus_tag="N7B39_004934"
     CDS             69..1211
                     /locus_tag="N7B39_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HGU2424727.1"
                     /translation="MTNKHPSLFSPFMLTEKIKLRNRIVMAPMTTWSANPDGTISEQE
                     LEFYKRRSQNVGLVITGCTYVTPSGIGFTHEFAAYDDRFINSLEKLAAAAKSGGAPAI
                     LQIFHAGNKAIPELVPNNDVISASASSVKSGDFMKRVVQSREMTENEIQETIRAFGDV
                     TKRAIKAGFDGIELHGAHGFLLQNFFSPLFNQRNDRWGGDLEGRMRFPLAVLQEVKNV
                     VYEYATKPFAIGYRISPEESATGGLRIEDTYKLLDRLISSGISYIHTSLVSINDSYPV
                     ESPNGPRTIELILNHIAGRVPVIAAGKIRTPSQAQEAISTGLPLVAIGKGLVINPEWV
                     TLAESGRGHEIQTTLNPQLVPELTIPDKLWDQIQASKGTGWFPLMD"
BASE COUNT          524 a          367 c          364 g          445 t
ORIGIN      
        1 caaagctggc aacaactaac cagttaaaac atacgcatca aaatcagata aacatttgag
       61 gtattgttat gacaaataaa cacccatcat tgttttctcc ttttatgctg acagaaaaaa
      121 taaagctacg taatcgtatc gttatggctc ctatgacgac ctggtcagcg aatcctgatg
      181 gaaccatctc tgagcaagaa ctcgaatttt ataaacgtcg ctcacaaaat gttggtttag
      241 taataacagg atgtacctat gtcacgccaa gcggcattgg gtttactcat gaatttgccg
      301 cgtatgacga ccgtttcatt aacagccttg aaaagttagc agcggcagcc aaaagtggcg
      361 gtgctccggc tattttgcag attttccacg ccggtaataa agccatcccg gagcttgtac
      421 caaataacga tgttattagt gccagcgcat caagcgtaaa atctggtgac tttatgaaga
      481 gagtggtaca aagcagggaa atgactgaaa acgaaattca ggaaaccatt agagcattcg
      541 gagatgtcac aaaaagggcg attaaagcag gttttgatgg cattgaactt catggtgcgc
      601 acggattcct tctacagaat tttttctccc ctctatttaa ccagcgtaat gaccgatggg
      661 gaggagatct cgaaggacgt atgcgcttcc cgctggccgt attacaggaa gtaaaaaatg
      721 tagtctatga atatgcgaca aagccctttg ccatcggtta cagaatatct cctgaagaat
      781 ctgcaacagg ggggctgcgg atagaggata cttataaact ccttgatcgg ctcatttcat
      841 ccggtatttc gtatatacat acctcgctgg tcagtattaa cgatagctac cctgttgagt
      901 cgccaaatgg cccccgaact attgaattga ttttaaatca tattgcaggc agagtgccgg
      961 taatcgctgc aggtaaaata cgcacaccgt ctcaggctca agaagcaatt tccactggcc
     1021 tgccacttgt tgcgatcggt aaagggctgg tgataaatcc agaatgggtg actttagctg
     1081 aatcaggtag aggccatgaa attcaaacaa cccttaatcc acaacttgtt cctgaattaa
     1141 ccattccgga taaactatgg gatcaaattc aggcatcgaa aggaactggc tggtttccgt
     1201 taatggatta aatatctatt actctcccga gagtaaaact atcaaatcaa tgagtaaccg
     1261 atacagcctc acaaattctt tcagataatt aacccgtcac tgcggcacgt tctctcataa
     1321 ggcgtgcctg cagtgtaatc tagtatgtat ccggcatagt tgacggttta atagagctca
     1381 attcctcgcc caggttacca acaacgatat cttgtataac accccaggag atacgacatt
     1441 atgatgagat gcgatatgct cctcagcgcc aatcaacgcc aggactgggc cagtcttgag
     1501 atcagactca ttcccggaac tgacttcagg cgacatatat caatgacgtt ggggcaataa
     1561 aagatgcctg atgtaataca tctattcaaa tttgtgatac atgtcaaaaa attggttgac
     1621 caaactcgtt atttatataa gggcacttac gaagtgcgct cttttttaaa gcgaggaagt
     1681 accaatgaaa gagaataaag
//
LOCUS       DBFIFI010000389         1695 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.391, whole genome shotgun sequence.
ACCESSION   DBFIFI010000389 DBFIFI010000000
VERSION     DBFIFI010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.391"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            89..343
                     /gene="bssS"
                     /locus_tag="N7B39_004935"
     CDS             89..343
                     /gene="bssS"
                     /locus_tag="N7B39_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HGU2424728.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            458..1576
                     /gene="solA"
                     /locus_tag="N7B39_004936"
     CDS             458..1576
                     /gene="solA"
                     /locus_tag="N7B39_004936"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HGU2424729.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDDLSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETADGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFSLSRFQ"
     gene            1624..>1695
                     /locus_tag="N7B39_004937"
     CDS             1624..>1695
                     /locus_tag="N7B39_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2770 family protein"
                     /protein_id="HGU2424730.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWG"
BASE COUNT          435 a          418 c          417 g          425 t
ORIGIN      
        1 ttatttattg gtatactgaa gttgcttcag ataaaaacgc attgaacctc gaataacgtt
       61 gtctagtaac acgaattagg gggccatgat ggaaaaaaat aatgaagtca ttcagactca
      121 tccgctcgta gggtgggaca tcagcaccgt tgatagctat gatgcgctga tgttgcgttt
      181 gcactaccag accccaaata agtccgagca ggaagggact gaagttggtc agacgctctg
      241 gttaaccact gatgttgcca gacaatttat ttcgatatta gaagcaggaa tcgccaaaat
      301 tgaatccggt gatttcccgg taaacgagta tcggcgtcat taacatgaca tgaccatcca
      361 cccaaaaggc acctcaccgg tgcctttacc atttctgggt tgtatatctg cattcagtta
      421 attactctgc ttaaaccatg agagcagaga gaaaaaaatg aaatacgatc tcatcattat
      481 tggcagcggt tccgtaggcg ctgccgccgg gtattatgca acccgcgccg gtttaaacgt
      541 gcttatgacc gacgcccata tgccaccaca tcaacacggc agccaccacg gcgatacgcg
      601 attaattcga catgcttatg gtgaaggcga aaagtatgtc ccgctggtcc tccgcgcaca
      661 aacgctgtgg gatgacctct cccgccacaa cgaagatgat cccatttttg tacgctctgg
      721 tgtcattaac cttggcccgg ctgactccgc atttctcgcc aacgtcgccc acagcgccga
      781 acagtggcaa ctcaacgttg aaaagctcga tgcgcaaggg attatggccc gctggccaga
      841 aatacgcgtc ccggacaact acatcggctt atttgagact gattccggtt ttttgcgcag
      901 cgaactggcg attaaaacct ggatccaact ggcgaaggaa gcgggctgtg cgcaactgtt
      961 caactgcccg gtcaccgcaa ttcgtcatga cgatgatggc gtaactattg aaacggctga
     1021 cggtgagtat caggcgaaaa aagcgattgt ctgcgcggga acatgggtaa aagacctgct
     1081 cccggagctg cctgtccagc ccgtacgtaa agtatttgcc tggtatcagg ccgatggccg
     1141 ctatagcgtg aagaataaat tcccggcgtt taccggtgaa ctgcccaatg gcgatcaata
     1201 ttatggtttt ccggcagaaa acgacgcgtt gaagattggc aaacataacg gaggccaggt
     1261 tatccattca gcggatgaac gtgttccgtt tgcggaagtg gtcagcgatg gttcggaagc
     1321 cttcccgttc ttgcgcaatg tattgccggg tatcggttgc tgcctgtacg gcgctgcctg
     1381 cacctatgat aattcgcctg acgaagattt tattatcgat accctacccg gccacgataa
     1441 tacactgctc attaccggcc tgagtgggca cggttttaaa tttgcgtcag ttttagggga
     1501 aatagctgcc gattttgcgc aagacaaaaa aagcgatttt gatttgacgc cattcagcct
     1561 ttcccgcttc caataatcat cattacggcc tccagaatga ggccgttttt acaggagtat
     1621 ttaatgcgtc ggctgttgca ctatctcatc aataatattc gcgagcatct gatgctatat
     1681 ctttttctct gggga
//
LOCUS       DBFIFI010000390         1690 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.392, whole genome shotgun sequence.
ACCESSION   DBFIFI010000390 DBFIFI010000000
VERSION     DBFIFI010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.392"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..>1690
                     /locus_tag="N7B39_004938"
     CDS             <1..>1690
                     /locus_tag="N7B39_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HGU2424731.1"
                     /translation="GAVTVAIRREYKQTWKTLDNELACALWPGDVDAGNIDGTIHRWA
                     TNEVIDKDREDWKRISASMRKQPEALGYDRQTIFGLVRERPIDIHKDPVALNKYISEY
                     LTTKGVFEHEETDQSSTDALQPSAAQTAPVETAESDTQKNEILVEAEPSVEREGPFYF
                     VFTDKGGEKYGRANKLSGLDKALAAGGTEISKEEYFARKNGTYTGLPQNVDTAEDSEQ
                     SEPVKVTADEVNKIMQAANISQPDADKLLAASRGEFVEGISDPNDPKWVKGIQSRDAE
                     DQNQPNVKQNEPEAEQNSPDTQQNGPEEQQPEPAVQQELEKVCTACGQTGGGNCPDCG
                     AVMGNATYLETFDEENQAEAQKNDPEEMEGTEHLHKENTGSDQYHASDNKTGETANPL
                     IKVNGHHEISSTSRLWHHLMIDLETMGKNPDAPINSIAGKFFDPATGEMGPEFSKTID
                     LETAGGVIDRDTIKWWLKQSREAQSSILTDEITLDDALLQFREFIDENSGEFFVQVWG
                     NGATFDNVILRRSYERQGIPCPWRYTNDRDVRTMVALGLVMDFDARTTIPFEGER"
BASE COUNT          522 a          396 c          438 g          334 t
ORIGIN      
        1 ccggggctgt cactgttgcc attcgccgcg aatacaaaca gacatggaaa acactcgaca
       61 atgaactggc ctgcgccctc tggcccggtg atgtggatgc aggaaacatt gacggtacca
      121 tccatcgctg ggcgacaaat gaggttatcg acaaggatcg cgaagactgg aagcgtatct
      181 cagcatcaat gcgcaaacag cccgaagcac ttggctatga ccgtcagact atttttggcc
      241 ttgttcgcga acgtccgatc gatattcaca aagatcccgt tgcactgaac aaatatatca
      301 gtgaatacct gacgacaaag ggcgtgtttg aacatgaaga aacagaccag agctctactg
      361 atgctctcca gccgtcagca gcacaaactg ctccagtgga gacggcagaa tccgatactc
      421 aaaaaaatga aatcctggtg gaagctgaac catctgtaga gcgtgaagga ccattttatt
      481 tcgtctttac cgataagggc ggggaaaaat acggcagggc aaacaaactt tctggtctgg
      541 acaaggcgct ggctgccggc ggtaccgaaa tctcaaaaga agaatatttt gcccgaaaaa
      601 atggcacata cacgggctta ccgcaaaatg tggataccgc tgaagattcc gaacaatcag
      661 agccggtaaa agttaccgct gacgaagtaa acaaaattat gcaggcagcc aatatcagcc
      721 agcctgacgc cgataaattg cttgctgcat cacgtggtga atttgttgaa gggattagcg
      781 acccgaatga tccgaaatgg gtgaaaggga tccagagtcg cgacgctgag gaccagaatc
      841 agcccaacgt gaaacaaaat gagccagaag cggaacaaaa cagcccggat acgcaacaaa
      901 acgggccaga agaacaacaa ccagaaccag cagtgcaaca ggaactggaa aaagtttgca
      961 ccgcatgcgg tcagaccggt ggcggcaact gtcctgactg tggtgcggtg atggggaacg
     1021 caacctacct ggaaacattc gatgaagaga atcaggctga agctcagaaa aatgatccgg
     1081 aggaaatgga aggcactgaa catctgcaca aggagaacac tggcagcgat cagtatcacg
     1141 ccagcgataa taaaactggc gaaacagcaa atcccttaat taaagtgaac ggtcatcatg
     1201 aaatctcatc caccagcagg ttgtggcacc atctgatgat tgaccttgaa acaatgggaa
     1261 aaaatcctga tgcgccaata aactctatag ccggtaagtt ttttgatccg gcaaccggag
     1321 agatggggcc agaattcagc aaaactatcg atctggaaac cgcaggtggg gtcatcgatc
     1381 gggacaccat taagtggtgg ctgaaacagt cacgcgaagc acaatcctcc attctgaccg
     1441 atgaaatcac gttggatgat gcactgctgc aattccggga atttatcgac gaaaactccg
     1501 gtgaattttt tgttcaggtc tggggtaacg gtgcaacttt cgacaacgtg attttacgcc
     1561 gttcatatga acggcagggg atcccctgcc cgtggcgtta caccaatgat cgcgatgtaa
     1621 gaacgatggt tgctctggga ctggtgatgg atttcgacgc aagaacgact attccattcg
     1681 aaggtgaacg
//
LOCUS       DBFIFI010000391         1685 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.393, whole genome shotgun sequence.
ACCESSION   DBFIFI010000391 DBFIFI010000000
VERSION     DBFIFI010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.393"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..1279
                     /gene="sctN"
                     /locus_tag="N7B39_004939"
     CDS             <1..1279
                     /gene="sctN"
                     /locus_tag="N7B39_004939"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system ATPase SctN"
                     /protein_id="HGU2424732.1"
                     /translation="LLNKYSYLHSINGSLIEAELDDVSVGEVCEIYASRQANERIARA
                     QVVGFRNGKTLLNLIGSSVGLTRTAVLKPTGEQLTIQISDAFLGSVLNASGQIMERFV
                     PDTPGDRGNLRLIDELPPSYQERRVINTPLETRIRVIDGVLTCGIGQRVGIFASAGCG
                     KTVLMHMLVNNTEADVFVIGLIGERGREVTECAESLKKSVNAAKCVLVYATSDFSSVD
                     RCNAALMATTVAEYFRDRGKRVVLFIDSMTRYARALRDMKLAAGEPPARRGYPASVFD
                     SLPRLLERPGPTLKGSITEFYTVLLEGEDESDPLGDEIRSILDGHIYLSRKLAGQGHY
                     PAIDVLKSVSRVFGQVTDEKHRDNAARVRKNLTTLEDLQVFIDLGEYRAGQNAENDFA
                     MNARPKLTNWLKQSVNEKMPMSETLKELERIVK"
     gene            1269..>1685
                     /locus_tag="N7B39_004940"
     CDS             1269..>1685
                     /locus_tag="N7B39_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
                     /protein_id="HGU2424733.1"
                     /translation="MLSKVNRLIRRTAQSLAACEASLQKLNAEKEKLAEKERLYDMQL
                     KNLQSLLDMKELLGEVVFRQDIFYSLRKVTVIQQQIAEINLEKQKIAERRKILNKEIV
                     QQQAQRKHWWLKGEKYDRLKKRIKKQLLNQMLYQDEL"
BASE COUNT          532 a          317 c          413 g          423 t
ORIGIN      
        1 attattaaat aaatacagct acttacatag cataaacggc tctctgattg aagcagaact
       61 tgacgacgtt tctgtgggtg aagtatgcga aatttatgcg agcaggcaag caaatgaacg
      121 tatagccagg gctcaggtcg ttggctttcg caatgggaag acattattaa accttatagg
      181 cagctctgtg gggcttacac gtactgcagt tttgaaacca acgggagagc agttaaccat
      241 acagatcagt gatgcctttc ttggttcggt tttgaatgcc tctggtcaaa ttatggaaag
      301 gtttgtcccg gacactccag gcgatagggg aaatttacgc ctgattgatg aactgccacc
      361 gtcgtatcag gaacgtcgag ttattaatac accacttgag acacggataa gagtcattga
      421 tggcgtactg acatgtggca ttggtcaacg tgttgggatt ttcgcctcgg ctggctgtgg
      481 caaaacggtt ttaatgcata tgctcgtaaa caatactgaa gctgatgtgt ttgttattgg
      541 cttgattggc gaacgtggta gggaagttac ggaatgcgcg gaatcgctca aaaaatcagt
      601 aaatgcagcg aaatgcgtat tggtctatgc cacctctgat ttttcgtcag tagaccggtg
      661 taatgccgcc ctgatggcga caaccgtggc agagtatttt cgagatcgag gaaagagagt
      721 tgtacttttt atcgactcaa tgacgcggta tgcacgagca ctacgtgata tgaaactggc
      781 agcgggtgaa ccacctgcca gacgtggtta tcctgcttct gtttttgaca gtttgcctcg
      841 cttactggag aggccgggac caacactaaa aggaagtatt accgagtttt ataccgtgtt
      901 actggaaggg gaagatgaat cagacccact gggtgacgaa attcgctcaa tccttgatgg
      961 ccatatctat ctaagtcgca aactcgcggg tcaaggtcac tatccggcaa tagatgttct
     1021 aaaaagtgtg agtcgagtat ttggtcaggt cactgatgaa aaacacagag ataatgctgc
     1081 acgggtaagg aagaacctca caactttgga agatctacag gtgtttattg acttagggga
     1141 atatcgcgca gggcaaaatg ctgaaaatga ttttgctatg aatgcccggc ctaaactcac
     1201 aaattggttg aaacagtcag ttaatgaaaa aatgccaatg agtgaaacgc taaaagagct
     1261 tgaacgaatt gttaagtaaa gttaatcgac ttatcagaag aacagcgcag agcctggctg
     1321 catgtgaagc atcattacaa aagttaaatg cagaaaaaga aaagttagcc gaaaaggaac
     1381 gcctgtatga tatgcaactt aaaaacctgc agtcactgtt agatatgaag gagttactcg
     1441 gcgaagttgt atttcgccag gatatatttt actcgttacg taaagttaca gtcattcagc
     1501 agcagatcgc tgagataaat cttgaaaagc aaaaaatagc cgagcggcga aaaatactta
     1561 acaaggaaat agttcaacag caagcacaaa gaaaacactg gtggctaaaa ggagaaaaat
     1621 acgaccgact aaaaaagcgc atcaaaaaac aacttttaaa tcagatgctt tatcaagacg
     1681 agctt
//
LOCUS       DBFIFI010000392         1651 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.394, whole genome shotgun sequence.
ACCESSION   DBFIFI010000392 DBFIFI010000000
VERSION     DBFIFI010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.394"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(39..887)
                     /locus_tag="N7B39_004941"
     CDS             complement(39..887)
                     /locus_tag="N7B39_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HGU2424734.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKTGAGICTPMITRAHNRAKAVFLC
                     VKRSHIQIMVGRMGPFSGGPDSLVTGSANPVRLTTHEICTSGGEFTNLSLEAAIMATI
                     PTLSHPDVTIENGRAVTTSVAVAEFFRKLHKNVIQKIEALECSSEFTELNFKPSEYTD
                     STGRKLPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEAELRQNNTTPTNKII
                     PGDGRTLVVRFDKFGNVEFTETVPDGALVCTLDTFRLYLEKQGWTLVNRSAIKNMTVE
                     QLLSIK"
     gene            complement(1316..1468)
                     /locus_tag="N7B39_004942"
     CDS             complement(1316..1468)
                     /locus_tag="N7B39_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HGU2424735.1"
                     /translation="MQKIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTETGARRW
                     LAKNTS"
BASE COUNT          414 a          392 c          386 g          459 t
ORIGIN      
        1 ccttaataaa tttctcgtat taagaaaatt ccagaaaact atttaatact cagcaactgc
       61 tcgacggtca tatttttaat tgcgcttcgg tttacaagag tccagccctg tttctccaga
      121 taaaggcgga aagtgtccag ggtacagacg agagcgccat caggaacggt ttcagtgaat
      181 tcgacgttgc cgaatttgtc gaagcgaaca accagagtgc gcccatcgcc cggaatgatt
      241 ttgtttgtgg gagtggtatt attctggcgt agctctgctt ccatgcgatc gaactcagcg
      301 atgtaggctt ctttaaatgc agcggctttt ttgccggtga agcccatcac caggaaaacg
      361 aagccgtttt tggtgatttg gtacattggg agtttgcgcc cggttgagtc ggtgtattca
      421 cttggcttaa aattaagctc agtgaattct gaggaacatt ccagagcttc aattttttga
      481 atgacgtttt tatgcagctt gcggaaaaac tctgcaactg caacagacgt agtgacagca
      541 cgaccatttt cgatggttac gtcagggtga gaaagggtag ggatagtagc catgatggca
      601 gcctctagtg ataagttggt aaactcacca ccggaggtgc aaatctcatg ggtggtgaga
      661 cgtacagggt ttgcactacc ggtcactaga gaatccggcc cgcccgaaaa cggccccata
      721 cgccccacca taatctgaat gtggctgcgc tttacgcata aaaaaaccgc tttggcgcgg
      781 ttatgcgctc tagtgatcat cggggtgcaa atcccggcac ccgttttatg aggtgcaggt
      841 gcactataat tccacccgtt ctggttttca atagctacat tcaacatttt ctctcacctt
      901 tcatcactga agtgaacttt gttgatgcgg tgcctggtgc ctccaggtga cgttaaccag
      961 ttaacaatta acgccggata tttcacccat aactctgcta cgaaggacaa tagcttttta
     1021 actgttccgc gtgcgcttag ccgcattcac cgcatcacaa aattcacttt aaaaagggcg
     1081 gacatcagtc gaacttcaag aaaaaactga tgccgccaag actacacaca gcagtgttgt
     1141 tattcacaac cggaggcgca ctcccaccat ttaaatttga cagacaagac cgactcttta
     1201 tggataccgg aaatgcgcct tcgtgttatg cccggtttta tttcaccacc tccgggcttt
     1261 ggtggcctcg gctatacctc tacagcgaga atattgaatt aatccaataa atggattagc
     1321 tggtattttt ggcaagccag cgacgtgcgc ctgtttcagt tttaaatgtt ttgcttttgg
     1381 tatacgtcat ggcggtgaac gttccatcct ggttggggaa cacgccacac actagggatt
     1441 cgttgttgcc gaggtcgatt ttttgcattt tgcgaatctc acatcttgtt gctgcatata
     1501 gcggcttctg cctgccagag atcccagtcg ttgctgcgta gagcctgtac agcttggctg
     1561 taagtgatac cgcaacaatc catcaaatac tgaactactt cgtaatgcac cattttatct
     1621 ctccctttaa cgccgggtgg cggaactaaa a
//
LOCUS       DBFIFI010000393         1642 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.395, whole genome shotgun sequence.
ACCESSION   DBFIFI010000393 DBFIFI010000000
VERSION     DBFIFI010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.395"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..333
                     /locus_tag="N7B39_004943"
     CDS             <1..333
                     /locus_tag="N7B39_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22AR C-terminal domain-containing protein"
                     /protein_id="HGU2424736.1"
                     /translation="QCEKNTGKQEKKLNGLSAKETDSLVWLWDYANRSQALFRELYPA
                     LKLIQSGYSGICHDYGYEFSYIIGRARGVLINHTRDIDIYEPDGPTNLLAWERLKNKE
                     LPPSLHRY"
     gene            408..1130
                     /locus_tag="N7B39_004944"
     CDS             408..1130
                     /locus_tag="N7B39_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HGU2424737.1"
                     /translation="MNELINSNAIKMTSIEIAELVGSQHGNVRISIERLAKRGVIQLP
                     SMQKVENKQTISPNKFTSVYIFEGEQGKRDSIIVVAQLSPEFTARLVDRWRELEGATA
                     KIPQTFSEALRLAADLEDQKAELEKQLALAAPKVEFADRVGEASGILIGNFAKVVGIG
                     PNKRFAWMRDHKILIASGARRNVPMQEYMDRGYFTVKETAVNTNHGIQISFTTKITGR
                     GQQWLTRKLLDNGMLKVTGEAA"
     gene            1130..>1642
                     /locus_tag="N7B39_004945"
     CDS             1130..>1642
                     /locus_tag="N7B39_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HGU2424738.1"
                     /translation="MAKPARRKCKICKEWFHPAFSNQWWCSPEHGTKLALERRNKERE
                     KAEKTAEKKRRREEQKQKDKIKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCISC
                     GTLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELINRIGQE
                     AVDEIESNHNR"
BASE COUNT          521 a          369 c          408 g          344 t
ORIGIN      
        1 cagtgcgaga aaaatactgg caaacaagag aagaagctca acgggctttc cgcaaaagaa
       61 acagacagcc ttgtatggct gtgggattat gccaaccgct cacaggcatt gttccgtgag
      121 ttgtatcccg cattaaaact gattcagtct ggctattccg gcatatgcca cgactacggc
      181 tatgagttct cgtatatcat cgggagggcg aggggcgttt taattaatca cacgcgggat
      241 atagatattt atgagcctga cgggccgacg aaccttctgg catgggaaag gcttaagaac
      301 aaagagttgc cgccttcact gcatcgctac tgacaattga caacttaaca aacccagctt
      361 cggctgggtt ttttattgct gaattttcaa tgtgagagga catgacaatg aatgagctga
      421 taaatagcaa tgccatcaaa atgacaagca ttgaaatcgc tgagttggtg ggaagccaac
      481 acggtaatgt cagaatatca atagaacgtc tggcaaagcg tggggtgatt caacttcctt
      541 caatgcaaaa agttgaaaat aaacaaacaa ttagccctaa caaattcaca agcgtgtata
      601 tattcgaagg cgaacaaggt aagcgagaca gcattattgt cgtcgctcag ttgtcgccgg
      661 aattcaccgc tcgccttgtt gaccgctggc gagaactcga aggggcaacc gcgaaaatac
      721 cacaaacctt ttctgaggca ttgcgccttg cggccgacct tgaagaccag aaggctgaac
      781 tggagaaaca gcttgctctc gcagcaccta aagttgagtt tgccgatcga gttggcgagg
      841 ccagcggaat tttgattgga aactttgcaa aggttgttgg tattggtcca aacaaacggt
      901 ttgcgtggat gcgcgatcac aaaatcctta ttgcttcagg tgcccggcgc aatgtgccaa
      961 tgcaggaata tatggatcgc ggctatttca cagtgaaaga aacagcggtc aatacaaatc
     1021 acggaataca gatatcgttc accacaaaaa tcaccgggcg tggtcaacag tggctgacaa
     1081 gaaagctgct agataacgga atgcttaaag taacagggga ggctgcttaa tggctaaacc
     1141 agcgcgaagg aaatgcaaaa tctgtaagga atggtttcac ccggcattct caaatcagtg
     1201 gtggtgcagc ccggaacacg gaactaaatt agcactcgaa cgacgaaata aagaacgcga
     1261 aaaggcggaa aaaacagcag agaagaaacg acgacgagag gagcagaaac agaaagataa
     1321 aattaagatt cgaaaactcg ccttaaagcc ccgcagttac tggattaaac aagcccaaca
     1381 agccgtaaac gccttcatca gagaaagaga ccgcgactta ccatgtatct cgtgcggaac
     1441 gctcacgtct gctcagtggg atgccggaca ttaccggaca actgctgcgg cgcctcaact
     1501 ccgatttgat gaacgcaata ttcacaagca atgcgtggtg tgcaaccaac ataaaagcgg
     1561 aaatctcgtt ccgtatcgcg tcgaactgat taaccgcatc gggcaggaag cagtagacga
     1621 aatcgaatca aaccataacc gc
//
LOCUS       DBFIFI010000394         1596 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.396, whole genome shotgun sequence.
ACCESSION   DBFIFI010000394 DBFIFI010000000
VERSION     DBFIFI010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.396"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(1..123)
                     /locus_tag="N7B39_004946"
                     /pseudo
     CDS             complement(1..123)
                     /locus_tag="N7B39_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309595.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(134..1362)
                     /locus_tag="N7B39_004947"
                     /pseudo
     CDS             complement(134..1362)
                     /locus_tag="N7B39_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
     gene            1454..1596
                     /locus_tag="N7B39_004948"
                     /pseudo
     CDS             1454..1596
                     /locus_tag="N7B39_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          354 a          449 c          372 g          421 t
ORIGIN      
        1 tggacggtag tctttccact ctggtgtatc taatattctt tctgctggta tctctttgcc
       61 atgcagataa aattttatgt ttttcattat ttggctgttg tctttattac gataaatcca
      121 tctggatttg cccctatatt tccagacatc tgttatcact taacccatta caagcccgct
      181 gccgcagata ttcccgtggc gagcgataac ccagcgcact atgcggatgc cattcgttat
      241 aatgctcgaa cgcctctgca aggttctttg ctgccgttaa cccgtctggt ttgggcatga
      301 tactgatgta gtcacgcttt atcgttttca cgaagttctc tgctattccg ttactctccg
      361 gactccgcac cgccgtgttc ttcggttcaa gtcccaacat ccgggcgaac tggcgtgttt
      421 cattagcccg gtagcatgaa ccattatccg tcagccactc cactggagac gacggaagat
      481 cgttgccgaa gcggcgttcc accgctccca gcatgacgtc ctgtactgtt tcactgttga
      541 agccgccggt agtcaccgcc cagtgcagtg cctcacgatc acagcagtcc agcgcgaacg
      601 tgacacgcag tctctctccg ttatcacagc agaactcgaa cccgtcagag caccatcgct
      661 gattgctttc tttcacggcc actctgcctg tatgtgcccg tttcgatggc ggtacagtag
      721 gttttcgctc aagcaacagc gcattctggc gcatgatccg gtaaacacgt ttggcattga
      781 tcgcaggcat accatcaagt tctgcctgtc tgcgaagcag cgcccatacc cgacgataac
      841 catacgtggg cagctctccg ataacatggt gtatacggag aagcacatcc gtatcatcag
      901 tgtgacgact gcggcggcca tccatccagt catcggttcg tctgagaatg acgtacaact
      961 gcgcacgcga cacccggaga caacggctga ctaagcttac tccccatccc cgggcaataa
     1021 gggcgcgtgc gctatccact tttttgcccg tccatattca acggcttctt tgaggagttc
     1081 attttccatc gttttcttgc cgagcaggcg ctggagttct ttaatctgct tcatggcggc
     1141 agcaagttca gaggcaggaa caacctgttc tccggcggcg acagcagtaa gacttccttc
     1201 ctagtattgc ttacgccaga gaaataactg gctggctgct acaccatgtt gccgggcaac
     1261 gagggagacc gtcatccccg gttcaaagct ctgctgaaca attgcgatct tttcctgtgt
     1321 ggtacgccgt ctgcgtttct ccggccctaa gacatcaatc atctgttctc caatgactag
     1381 tctaaaaact agtattaaga ctatcactta tttaagtgat actggttgtc tggagattca
     1441 gggggccagt ctagtcatct acacaaccaa cgccatcgag tcgctgaaca gcgtaatccg
     1501 gcatgccatc aaaaaacgca aggtgttccc gacggatgac tcagtgaaaa aggtcgtgtg
     1561 gctggcaatc caggcggctt cacagaaatg gacgat
//
LOCUS       DBFIFI010000395         1586 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.397, whole genome shotgun sequence.
ACCESSION   DBFIFI010000395 DBFIFI010000000
VERSION     DBFIFI010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.397"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..650
                     /locus_tag="N7B39_004949"
     CDS             <1..650
                     /locus_tag="N7B39_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU2424739.1"
                     /translation="LAKTVAAPIMATGVGAALMYATSSGPYYAQSLSGSPDIEMIKKR
                     MAELFNQSDTSVRNKYRPAFDKWNKLVDKLNHERNTVPFACLTTIIATAINETPEGDT
                     NVAVVMGCKSAKDRTISIVLGNSMLQTLFEKRLADDGEIENLFDQQGYFSCDSLTAEE
                     VMMLKDLFDIRVLHLSNKFNVGLQGNINTDVLQDSFFKNVDFIHESNSYTVGMGV"
     gene            1344..1562
                     /locus_tag="N7B39_004950"
     CDS             1344..1562
                     /locus_tag="N7B39_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HGU2424740.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
BASE COUNT          491 a          297 c          366 g          432 t
ORIGIN      
        1 cccttgccaa aaccgtggct gcacccatca tggcgacagg tgtgggcgcg gctttaatgt
       61 atgcaacatc atcaggaccg tattatgcgc aaagtctttc tggttcgccg gatatcgaga
      121 tgataaaaaa acgcatggct gaattgttta accagtcgga tacttctgtg cgcaacaaat
      181 atcgcccggc ttttgataaa tggaataaac tggtagataa attaaatcac gaaagaaata
      241 cagttccgtt tgcgtgtttg accaccatta tagcgacagc gataaatgaa actcctgagg
      301 gggatacgaa tgttgccgtt gtgatggggt gtaaatctgc gaaagatcgc acgatatcca
      361 ttgtccttgg caactcgatg ttgcaaacac tctttgaaaa acgcctcgcc gatgatggag
      421 aaatagaaaa cctttttgat caacagggat attttagttg cgattcatta acagccgaag
      481 aagtaatgat gttaaaagac ctttttgata ttcgagttct tcatctctct aacaaattta
      541 atgttgggtt gcaaggtaat atcaacacgg atgttttaca agattcgttt tttaaaaatg
      601 tcgatttcat tcatgaatct aactcgtata ccgtagggat gggagtctga agagtaaaac
      661 tcattaggat tatcagaatt cggccaatgc attgcaaaaa tgaaatacaa agagagatgt
      721 gtgcaggcgc ttagccgcga ggggcatggc gaagccatac agaaagcacc ggcattcaga
      781 actcctgtga aagcggcata gtgccgctgc tcaggagttt tgttatgacc cgtatcgtgc
      841 tgaacgttgc caccctatga tgcgattaaa aaaactcacc gtcgagccga tgattgacgt
      901 caatgctatc ggatgatgcc gggggtgttc cggaaggaat tccttgtttc ccgaactgtt
      961 accaacaaaa ttataacgat gagcaagtaa agaccggttg gtggcagtac tgaccggtct
     1021 gtgatggaag attaatttca ccttaaatcc tggtgctgtt aggatgctga cgtttttcgg
     1081 gtacaccatt cagattatgc aaataaagat tcaatgaaaa aataaatatc cctgaatata
     1141 atatattcgt agcacccata actcatcctg ggcgataact aaaccatttt tgagtaacaa
     1201 atgaaacaac tgcaacgctc ttattatttc tggaaaacgt ctcgcaaaaa caaaacaaca
     1261 tgcaaaaatc cgctttcgtt aattaatttt tcggcgtaat tttcgcacat acattaatga
     1321 gtaatatatg taaggtggtg gtaatggcga tgaatacggt ttttcttcat ttatcagaag
     1381 aggcaattaa acggctgaac aagcttcggg gctggcgtaa ggtttcacga tctgcaattt
     1441 tacgcgaagc ggtagagcaa tatctggaac gtcagcaatt cccggtgcgt aaagcaaagg
     1501 gaggcaggca aaagggggag gtagttggtg ttgatgatca gtgtaaggag cataaggaat
     1561 gacaaaaatt tttcattctt gaatat
//
LOCUS       DBFIFI010000396         1585 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.398, whole genome shotgun sequence.
ACCESSION   DBFIFI010000396 DBFIFI010000000
VERSION     DBFIFI010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.398"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..>1585
                     /gene="espL1"
                     /locus_tag="N7B39_004951"
     CDS             <1..>1585
                     /gene="espL1"
                     /locus_tag="N7B39_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HGU2424741.1"
                     /translation="TALFTINFATSPIHAENIFITLHYKKEIISLLLETTPIKANHLR
                     SILDYIEQEQLTAENRNHCMKLSKKIHREKTIQPTVNLNGSAFFSQSPSDAIFCRHLS
                     LQYALDSLRNGKGKVNLIKHYSSVESIQQHVPLVRDAEFRALLRHPPAGSRVIASKDF
                     GFALDIFFCRMMANNVSHMSAILYIDNHTLSVRLRIKQSAYGQLNYVVSVYDPNDTNV
                     AVRGTHRTARGFLSLDKFISSGPDAQTWADRYVRNCAIAILPLLPEGVPGAIFTGIAT
                     RMPFAPIHPSAMLLIMATGQTQQLITLFRQLPILPEKEIIEIITAQNSIGTPALFLAM
                     MNGHTDNVKIFMQEIQSLVDNHIIHEDNLVKLLQTKSANETPGLYISMLYGFDEIIDI
                     FLNALTTPIAQELLNKKLVMSILAMKIHDGEPGLYAAMENNHPLCVTRFLSKINGIAF
                     KYKLSKANIMDLLKGATAQGTPALYIAMSKGNEDVVLSYISTLGAFAKKHSFSQHQLF
                     TLLAAKNHDNMSAVHIAIHH"
BASE COUNT          489 a          342 c          294 g          460 t
ORIGIN      
        1 actgcccttt ttaccattaa ctttgccact tcacccatcc atgcagaaaa tatattcatt
       61 acattacact ataaaaaaga aatcatttcc ttattactgg aaaccacgcc tattaaagct
      121 aaccatttgc gaagcatact ggattatatt gaacaggaac agttaactgc cgaaaatcgt
      181 aaccattgta tgaaactgtc taaaaaaatc catagagaaa aaactataca accaacagta
      241 aatctcaatg gtagtgcatt tttttcgcaa tctccttctg acgctatttt ttgtcgccat
      301 ctgtcattgc aatacgcact tgattcattg agaaatggaa aaggcaaggt caacctgatt
      361 aaacattact cctccgttga atccatacag cagcatgtcc ccttagtccg ggacgcggag
      421 ttcagagcat tacttcgcca tcctcctgca gggagtcgcg ttatcgcgag taaggatttt
      481 ggcttcgctt tagatatttt cttctgtcga atgatggcaa acaatgtcag tcatatgtct
      541 gcgattttat atatagacaa tcatactttg tcagtaaggc tacgaataaa gcagtcggcg
      601 tatgggcaat taaattatgt tgtgtccgtt tacgacccga acgataccaa cgttgccgtc
      661 agaggcaccc acaggacagc acggggcttt ctctcgcttg ataagttcat cagttcaggt
      721 cccgatgctc agacctgggc tgataggtat gttcgcaact gtgcaattgc tattctgccc
      781 ctattacctg agggagttcc aggggctatt ttcacgggta tcgcgacacg aatgccattt
      841 gcccctatac atccatcggc aatgttgtta ataatggcca caggccagac tcaacagctt
      901 attacattat tcagacagtt gcccatactc cctgaaaaag aaatcattga aataataact
      961 gcgcagaata gcattggtac acctgcttta tttttggcta tgatgaacgg acatactgac
     1021 aacgtgaaaa tatttatgca agaaattcag tcactggtag ataatcacat cattcatgaa
     1081 gataatctgg ttaaattgtt gcaaactaaa agtgctaacg aaacacctgg actttatatc
     1141 tccatgttgt atggattcga tgagataatc gatatctttc tgaatgcatt aaccactcct
     1201 atagcacaag agcttttaaa caaaaaactg gtgatgagta ttttagccat gaaaatacat
     1261 gatggtgagc caggattata cgccgcaatg gaaaataatc accctttgtg tgtcacacgg
     1321 ttcctctcta aaattaatgg catcgccttt aaatacaagt tgagcaaagc taacatcatg
     1381 gatttattaa aaggcgctac agcacaggga acccctgcat tatacatcgc tatgagcaag
     1441 ggtaatgaag acgtcgtgtt atcttatata tcgacgctgg gtgcttttgc aaaaaaacat
     1501 tcttttagtc aacatcagtt atttacactg ttggctgcta aaaatcatga caacatgtca
     1561 gctgttcata tagccattca tcata
//
LOCUS       DBFIFI010000397         1585 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.399, whole genome shotgun sequence.
ACCESSION   DBFIFI010000397 DBFIFI010000000
VERSION     DBFIFI010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.399"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..137)
                     /locus_tag="N7B39_004952"
     CDS             complement(<1..137)
                     /locus_tag="N7B39_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0019836 - hemolysis by symbiont of host
                     erythrocytes [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga-like toxin beta subunit"
                     /protein_id="HGU2424742.1"
                     /translation="MKKMFMAVLFALVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     K"
     gene            complement(149..1108)
                     /locus_tag="N7B39_004953"
     CDS             complement(149..1108)
                     /locus_tag="N7B39_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HGU2424743.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     ESQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
     gene            complement(1199..1275)
                     /locus_tag="N7B39_004954"
     tRNA            complement(1199..1275)
                     /locus_tag="N7B39_004954"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1239..1241),aa:Arg,seq:tct)
     gene            complement(1289..1365)
                     /locus_tag="N7B39_004955"
     tRNA            complement(1289..1365)
                     /locus_tag="N7B39_004955"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1329..1331),aa:Arg,seq:tcg)
     gene            complement(1375..1450)
                     /locus_tag="N7B39_004956"
     tRNA            complement(1375..1450)
                     /locus_tag="N7B39_004956"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1415..1417),aa:Ile,seq:cat)
BASE COUNT          435 a          404 c          336 g          410 t
ORIGIN      
        1 tcttttccgg ccacttttac tgtgaatgta tcattctcat tatacttgga aaactcaatt
       61 ttacctttag cgcaatccgc cgccattgca ttaacagaaa ctaatgcaaa taaaaccgcc
      121 ataaacatct tcttcatact taactccttt atttacccgt tgtatataaa aactgtgact
      181 ttctgttcag aaacgctgca gctgtattac tttcccataa tgtattgttt atttttataa
      241 cgggcctgtc gccagttatc tgacattctg gttgactctc ttcattcacg gcgcgaacag
      301 aacgcgcccc ctgatgatga caattcagta taacggccac agtgcccagt atcgccgata
      361 tattattaaa ggatattctc cccactctga caccatcctc tccccgatac tccggaagca
      421 cattgctgat tcgcccccag ttcagagtga ggtccacgtc tcccggcgtc atcgtataca
      481 caggagcagt ttcagacagt gcctgacgaa attctctctg tatctgcctg aagcgtaagg
      541 cttctgctgt gacagtgaca aaacgcagaa ctgctctgga tgcatctctg gtcattgtat
      601 taccactgaa ctccattaac gccagatatg atgaaaccag tgagtgacga ctgatttgca
      661 ttccggaacg ttccagcgct gcgacacgtt gcagagtggt ataactgctg tccgttgtca
      721 tggaaaccgt tgtcacaccg ggcactgata tatgtgtaaa atctgaaaaa cggtagaaag
      781 tatttgttgc cgtattaacg aacccggcca catataaatt attttgctca ataatcagac
      841 gaagatggtc aaaacgcgcc tgatagacat caagccctcg tatatccaca gcaaaataac
      901 tgcccggtgg ggtgtggtta ataacagaca ccgatgtggt cccctgagat atatgttcaa
      961 gaggggtcga tatctctgtc cgtatactat ttaacgaaga gacataactt tgttgggtcg
     1021 aaaagtctat cgtaaactcc cgggaatagg ataccgaaga aaaacccagt aacaggcaca
     1081 gtacccattt aaataatata cacttcatat acaggtgttc cttttggctg aagtaatcag
     1141 caccagaccc ggcgcagata taaaaaaggc ccgcaaaagc gagcctggta aataaatatg
     1201 gcgcgttgta ctggattcga accagtgacc gattgctcag aaggcaattg ctctgtccgg
     1261 ctgagctaac aacgcataat gcagataatg gaccgccatc gaggactcga accccgcgca
     1321 gccagcttcg aaggctggcg ctctgtcccg atgagctaat ggcggtatgt gatatggtgg
     1381 cccttgctgg atttgaacca gcgacctggc gattatgagt cgctcgctct caccactgag
     1441 ctaaagggcc gggcgcagga taataacggt acgtaactaa ttctgcaata tcatccgttc
     1501 tgactgacta cattctgaac ttccctgacc gtctgctcaa aacgcccgct ctccagctca
     1561 acgccaattg cacgacgccc tagcg
//
LOCUS       DBFIFI010000398         1584 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.400, whole genome shotgun sequence.
ACCESSION   DBFIFI010000398 DBFIFI010000000
VERSION     DBFIFI010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.400"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..1193
                     /gene="nleA"
                     /locus_tag="N7B39_004957"
     CDS             <1..1193
                     /gene="nleA"
                     /locus_tag="N7B39_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector NleA"
                     /protein_id="HGU2424744.1"
                     /translation="IPDDIQQHAPECGETTALLSLIKDKGLLSGLDEYIAPHLEEGSI
                     GKKTLDMFGLFNVTQMALEIPSSVSGISGKYGVQLNIVKPDIHPTSGNYFLQIFPLHD
                     EIGFNFKDLPGPLKNALSNSNISTTAVSTIASTGTSATTSTVTTEPKDPIPWFGLTAQ
                     VVRNHGVELPIVKTENGWKLVGETPLTPDGPKANYTEEWVIRPGEADFKYGASPLQAT
                     LGLEFGAHFKWDLDNPNTKYAVLTNAAANALGALGGFAVSRFASTDPMLSPHIGAMVG
                     QAAGHAIQYNTPGLKPDTILWWAGATLGAADLNKAEFEVARFTDYPRIWWHAREGAIF
                     PNKADIEHATGADIRAMEEGIPVGQRHPNPEDVVIDIESNGLPHHNPSNHVDIFDIIQ
                     ETRV"
BASE COUNT          521 a          319 c          302 g          442 t
ORIGIN      
        1 atattccaga tgatatacag caacatgcac cggaatgcgg tgaaacaaca gctctactga
       61 gcttgataaa agataaaggt ctgctctcag ggctagacga atatatagct cctcaccttg
      121 aagaaggatc cataggaaaa aaaacattgg atatgtttgg tttattcaat gttacccaaa
      181 tggcattaga gatacctagt tccgtttcag gcatctctgg taaatatggt gtccagctaa
      241 acattgtaaa accagatatt catcctacat caggtaatta ttttttacag atattccctc
      301 tgcatgatga aataggtttt aattttaaag accttcctgg cccgttaaaa aatgcattaa
      361 gcaacagtaa tatatcaacc actgcagtgt cgactattgc atcgactgga acatcagcca
      421 ctacttcgac ggtaaccacc gagccaaaag acccaatacc atggtttgga ttaacagctc
      481 aagtggttcg taatcatggt gtagaacttc ctatagtcaa aactgaaaat ggatggaagc
      541 ttgttggaga aacaccactt actcctgatg ggccgaaagc aaattacacg gaggagtggg
      601 ttatcagacc gggagaagca gattttaaat atggtgcatc tccattacag gcaactctag
      661 ggctggagtt tggcgcacat ttcaagtggg atttagataa ccctaatact aaatatgccg
      721 ttcttaccaa tgctgccgca aatgcgcttg gtgctttagg gggatttgca gtatccagat
      781 ttgctagtac agatccaatg ttaagtcctc atatcggtgc aatggttggg caagcagcag
      841 ggcatgctat acagtataat acccctggat taaagccaga cactatttta tggtgggctg
      901 gtgcgacact gggggctgcc gatttaaaca aggccgagtt tgaagtagct agattcactg
      961 actatcctcg tatatggtgg cacgcaagag aaggagctat tttccccaat aaagcagata
     1021 ttgaacatgc cacaggtgct gatatacgcg caatggaaga aggtatccct gttggacagc
     1081 ggcatccaaa tccagaggat gtggtaatcg atatcgaaag caatggctta ccacatcata
     1141 atccatcaaa tcatgttgat atctttgata taatccaaga aacaagagtc taagctttaa
     1201 cttgtttatt ttaaaataat agctgaaatg gatggcagct attattaata aggattaata
     1261 tattttttct aaaatacccc acaaaacaaa cacatcataa aaattctaaa tacacacacc
     1321 taatctcaaa aaaaacaaac ttactatttg acttggatta tgtttttaat ccaatatttt
     1381 ccgacaacat ctccacagaa aaatgcattt acataaagac gccttcaagt catctgaaac
     1441 ctgccaacca cagacacctt cttatattct ggatgtctgc ggcgtttttt tacaaattac
     1501 tgacagccaa tactctctca gagaaaaata ttttaactat cttttataat gaagtttccc
     1561 ttgataggat cataaacaca ttta
//
LOCUS       DBFIFI010000399         1558 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.401, whole genome shotgun sequence.
ACCESSION   DBFIFI010000399 DBFIFI010000000
VERSION     DBFIFI010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.401"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..1386
                     /locus_tag="N7B39_004958"
     CDS             <1..1386
                     /locus_tag="N7B39_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU2424745.1"
                     /translation="QFNHWFGEEKPSPDLLCGYLSVPLKYTDTGGDASYEKKSQVKLA
                     LTKLPAKSKHKGSILIISGGPGLPGINPYINFDWPVTNLRESWDIIGFDPRGVGQSTP
                     TINCRQSDTETQENITEKQQVLNKINACIHNTGAEVIRHIGSNEAVYDIDRIRQALGD
                     KQLTAVAYSYGTQIAALYAERFPYNVRSIVLDGVVDIDDLEDNFTWQLKQAQSYQETF
                     DRFASWCARTKSCPLSSDRDKAITQFHELLSKLHHKPLLDSKGENISSDELISLTTDL
                     LLWRSSWPTLATAIRQFSQGIVSNEIETALSAPIASEESSDASGVILCVDQGDEQLTP
                     EERKFRKEALANAFPAINFDNGRSDSPDFCELWPIHSDLNKTRLKNTVLPSGLLFVAH
                     KYDPTTPWINARKMAEKFSSPLLTINGDGHTLALTGVNLCVDKAVVHHLITPQKIENI
                     YCPGNSEAEIQ"
BASE COUNT          495 a          331 c          312 g          420 t
ORIGIN      
        1 caattcaacc actggtttgg tgaggaaaaa ccgtctcctg acttactatg tggttatttg
       61 tctgttccat taaaatatac agacacaggc ggagatgctt cttatgaaaa aaaatcacaa
      121 gtcaaactag cgttgacaaa attgccggca aaaagcaagc ataaaggaag tatcctgata
      181 ataagtggtg gtcccgggtt accaggcata aatccttata ttaactttga ctggccagtc
      241 acaaatcttc gtgagtcatg ggatattatt ggatttgatc ctcgaggcgt cgggcagtcc
      301 actccgacaa taaactgccg gcaatcagat acagagactc aggaaaacat aaccgaaaag
      361 caacaagtat taaataaaat taatgcctgt atccataata ccggagccga agtcattcgc
      421 catatcggct ctaacgaggc tgtatacgat attgatcgta ttaggcaagc cttgggggat
      481 aaacaactga cagccgtggc gtattcgtat ggaactcaaa ttgcagcctt atatgcagaa
      541 cgttttccct acaacgtaag atctatcgtt cttgatggag tcgtcgatat cgatgacctg
      601 gaggacaact tcacatggca actcaaacag gcacagagtt atcaggaaac gtttgatcgc
      661 tttgcatcct ggtgtgcgcg tacaaaaagt tgcccgcttt cttcagacag agataaggca
      721 ataactcagt tccatgagct attatcaaaa ttacatcaca aacctttatt agacagtaag
      781 ggagaaaata tatcttcaga tgaactcata tcattaacaa cagaccttct gctatggcgt
      841 tcatcatggc caacccttgc aactgccata cgccagttct ctcaggggat tgtcagtaat
      901 gaaattgaaa ctgcgctcag tgctccgata gcctcagaag agtcaagtga tgcttcgggg
      961 gtaatccttt gtgtagatca gggggatgag caattaacac cagaagagcg aaaattccga
     1021 aaagaggctc ttgcgaatgc cttcccggct attaactttg acaatggacg ttccgattca
     1081 cctgattttt gtgaattatg gccaatacat agcgacctga acaaaactcg cctgaaaaat
     1141 actgttctgc cctctggttt actgtttgta gcacacaaat acgacccaac aacgccctgg
     1201 attaatgccc gtaagatggc agagaaattt tccagcccgt tactaacaat aaatggtgat
     1261 gggcatacat tagctctcac cggagttaat ttatgtgtag ataaagcagt tgtacatcac
     1321 ctgatcactc cacaaaaaat agaaaatata tactgcccag gaaattctga agcagaaata
     1381 caataatttc aaaatattcc gctatttgct cccaatgcaa aacatatatt gcattgggag
     1441 catattcata tttttgttat tcttgtatga aatcgtctca tgtacacggt ccttttttcg
     1501 ccgaaaactg atattacttt aatcaaaagt tgacgtgcca gattcgaagt aaatttat
//
LOCUS       DBFIFI010000400         1542 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.402, whole genome shotgun sequence.
ACCESSION   DBFIFI010000400 DBFIFI010000000
VERSION     DBFIFI010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.402"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(143..499)
                     /locus_tag="N7B39_004959"
     CDS             complement(143..499)
                     /locus_tag="N7B39_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424746.1"
                     /translation="MNKNLILAFALFSLPVFAEEDLGPGKYVCDIRISSLDTATQILS
                     KSATVLDNGNNFIVQMPNGDQLYSPDLENVDDGIKQKATIGGVTFIRRPTFNDRFIVE
                     DGNTGFFYKMRNCEKK"
     gene            complement(707..1267)
                     /locus_tag="N7B39_004960"
                     /pseudo
     CDS             complement(707..1267)
                     /locus_tag="N7B39_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195265.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
BASE COUNT          427 a          315 c          356 g          444 t
ORIGIN      
        1 atgtggttaa aataatagta tctatgaccg ctacggttaa cggtggttat tttgggaggc
       61 gttaacacgt agcggcttgt agttgtaagt cttgaacagg tacaaagcct gaaatcagga
      121 ggatatcgct atcaaacttc ctttatttct tctcgcagtt tcgcatctta tagaagaatc
      181 ctgtatttcc atcttccacg atgaagcgat cgttaaaagt tggacgacga ataaatgtca
      241 ctccacctat tgttgctttt tgttttatac catcatcaac attttccaga tctggagagt
      301 acaattgatc tccatttggc atttgaacga tgaaattgtt tccattatcg agaactgttg
      361 cggattttga cagaatttga gtagcggtat ccaggctgga aatcctaatg tcacaaacat
      421 atttgcctgg ccccagatct tcttctgcaa atacaggtaa ggaaaataat gcaaatgcga
      481 ggatgagatt cttattcatg caaactccag gaagatcaat aaaagcaata tgttacataa
      541 aaagcacgta aatgaaaaga gcgattggtt aatgagtaat gctattaatc cccgcatggc
      601 ctacttttca ttggctgcgg tgttgaacct cattgaatat gggcaagact ctgactgagt
      661 gaccgacgta aataggtaga tgcataatcg cttaccgccc tgtaactgca aacggtgccc
      721 aggcttctgg ctgaatacca cggctttgca tccacggaac ggcgtgtagc tgctgattga
      781 aagggttaac ttcaacctgg tttaccgctg gaacgacgtt attaaaggcg ataaggtcag
      841 ccagtcggtc aggatggaag ttactgacgc caatagcgcg aattttaccg gcctgttgca
      901 gctcttccat ggcacgccag gcaccgtgga catcaccgta aggctgatga atcaaataca
      961 ggtcaacgta atcaagttgc agtcgattca gagagcgttc gaactgggct ttggcccctt
     1021 cgtaactggt atcctgcaac cagagtttcg tggtgacaaa gagttcgtta cgggcgatac
     1081 cgctttgttt cagtgcgttc ccgacctggg gttcattttg ataggaggcg gcggtgtcaa
     1141 tcagtcgata accggtgtta atagcgtcaa tgaccgctct ttcgcattcg gcggcatcgg
     1201 tcatctgaaa tacaccaaag cccagcaggg gcatttcaat accgttattc agttttactt
     1261 tttgcatggt gttatccttc cgttatcgtt catgagaagc ataacgtaaa gagaagagct
     1321 cgattagacg gcaggatttg tcaggggtta tgaacgaaat tcataaatct gtttgagtgt
     1381 tgcgatgggt agtgcaagtt cgatatctcc gcaatttaca gtccgatgaa ggaaaatgaa
     1441 tatccataaa aaatatatcg gtttatcctg gcgtatatac ctacttcgac gtatttccaa
     1501 tagttgctaa ttaaaggcag gtcattatta ttcactctga at
//
LOCUS       DBFIFI010000401         1537 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.403, whole genome shotgun sequence.
ACCESSION   DBFIFI010000401 DBFIFI010000000
VERSION     DBFIFI010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.403"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            570..1343
                     /locus_tag="N7B39_004961"
     CDS             570..1343
                     /locus_tag="N7B39_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HGU2424747.1"
                     /translation="MGPTSVGLVPLIASSPNPVQSTASELRTSCGGSQSPIKEAAIMA
                     TIPALSQPEITITNGRVVTTSIAIAEFFDKRHDDVLKKIRLMDCSTEFNARNFADVTY
                     TDAKGEQRPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEKQLWEQKQLALSY
                     PAMNINFPLSWFSEHHPYATMNYVDRNTLQLDTSVLFDMPSPAMRIFNELRRNGYNVD
                     AAVAEFNAFKHLTEEMRRTLQDISRLSDRNSRKGFSLSL"
BASE COUNT          404 a          381 c          372 g          380 t
ORIGIN      
        1 tatattttac ccaagccaga aacatcgcgc ccttacacag ggctacataa atcagtcgta
       61 cggcgcgact taacccgccg caaaatgctc tttaacaatc tggagcttta cagcgtcaat
      121 gacctgttta gacccctaca cgtaaacgtg ctgtatcatc gggtgcgatc cggtcgatga
      181 gagagtatcc ccgcgcgaga gcgagaacgg cgtgagaacg ggcaacactg gcaggaagtt
      241 ggcgctgacc aatacaggga atgttttggg gtgtggtgaa gggttcatgg acgggaatat
      301 gtcgcacgta aagcggcgag gcccgaggga ctattgctga agataagtag gccgaatcgg
      361 gtcgaaatgg gtctcccacc tgccacatcg ccaaagcatt tccgagaaag gacggtattg
      421 actgcgtgtg atgtcggggg ctatatttgg cgaacacctt ataaaacggg tgtcgggatt
      481 aggaccccgc tgacaatcga ggcgatacag acgcgccagc gtcttttttt gtatcgatat
      541 acacgcacat cttatcaatg gtgggctgta tggggccgac ttcggtcggg ctggttccct
      601 tgattgccag tagtcctaac cctgtacagt ccaccgccag cgagcttagg acctcctgcg
      661 gtggttctca atcaccaatc aaggaggctg ccattatggc tactatccct gccctttctc
      721 aacctgaaat caccattacc aatggtcgcg ttgtcacaac gtctattgct attgctgaat
      781 tttttgacaa gcggcacgac gacgttttaa aaaaaatccg attgatggat tgttctacgg
      841 aatttaacgc ccgcaatttt gcggacgtta cctacaccga cgccaaaggc gaacaacgcc
      901 caatgtacca aatcaccaaa aacggattcg tcttcctggt gatgggcttc actggtaaaa
      961 aagcagctgc attcaaggaa gcctatattg ctgaattcga tcgcatggaa aaacaactat
     1021 gggaacagaa acaactggcc ctgtcttatc ctgcgatgaa tatcaatttc cctttatcgt
     1081 ggttttcaga gcatcacccc tatgccacga tgaactatgt tgatagaaat acattgcagc
     1141 ttgatacctc cgtcttattc gacatgccaa gccctgcaat gcgcatattt aatgaattac
     1201 gtcgtaacgg ctataacgtc gatgcagctg tcgccgaatt taacgccttc aagcatctga
     1261 cggaagaaat gcgccgtaca ctacaggata tttcaagact gtcagatcga aattcccgaa
     1321 aaggcttctc gttaagtctt taaagaacag atcgtttgac aatgctgcat taacggggat
     1381 atattctgcc tcatggtgct caaaacacct tagcaagtag cggttaccgc gcccgacaga
     1441 catgcggttt ttttgtgtcc agtcttcttg gtttatgacc gggcgtgcgg ctaatacaat
     1501 accagcaatg ggaatatgcc cgccgactac ttgcggt
//
LOCUS       DBFIFI010000402         1527 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.404, whole genome shotgun sequence.
ACCESSION   DBFIFI010000402 DBFIFI010000000
VERSION     DBFIFI010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.404"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..>1527)
                     /gene="ehxA"
                     /locus_tag="N7B39_004962"
     CDS             complement(<1..>1527)
                     /gene="ehxA"
                     /locus_tag="N7B39_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin EhxA"
                     /protein_id="HGU2424748.1"
                     /translation="ADELGIEIHRTERDDTAIANQFFGAAEKVVGLTERGVAIFAPQL
                     DKLLQKYQKVGSKIGGTAENVGNNLGKAGTVLSALQNFTGIALSGMALDELLRKQRAG
                     EDISQNDIAKSSIELINQLVDTVSSINSTVDSFSEQLNQLGSFLSSKPRLSSVGGKLQ
                     NLPDLGPLGDGLDVVSGILSAVSASFILGNSDAHTGTKAAAGIELTTQVLGNVGKAVS
                     QYILAQRMAQGLSTTAASAGLITSAVMLAISPLSFLAAADKFERAKQLESYSERFKKL
                     NYEGDALLAAFHKETGAIDAALTTINTVLSSVSAGVSAASSASLIGAPISMLVSALTG
                     TISGILEASKQAMFEHVAEKFAARINEWEKEHGKNYFENGYDARHAAFLEDSLSLLAD
                     FSRQHAVERAVAITQQHWDEKIGELAGITRNADRSQSGKAYINYLENGGLLEAQPKEF
                     TQQVFDPQKGTIDLSTGNVSSVLTFITPTFTPGEEVRERKQSGKYEYMTSLIVNGKDT
                     W"
BASE COUNT          407 a          370 c          279 g          471 t
ORIGIN      
        1 ccatgtatcc ttaccattta caataagaga tgtcatatat tcatatttac cactctgttt
       61 tctttctcta acttcttctc ctggggtaaa tgttggtgtt ataaatgtca aaacacttga
      121 tacattacct gttgaaaggt ctatggtccc tttttgagga tcaaacactt gttgtgtaaa
      181 ctccttcggt tgagcctcta aaagccctcc attttccaga taattaatat atgccttacc
      241 actctgactg cgatcagcat tacgggttat gcctgcaagt tcaccgatct tctcatccca
      301 atgttgctgg gttattgcga ctgctctttc tactgcatgc tgacgagaaa aatcagcaag
      361 caaagacaga gagtcttcta aaaacgcagc atgtcttgcg tcatatccat tctcaaaata
      421 atttttgcca tgctcctttt cccattcatt gatccgagca gcgaatttct ctgcaacgtg
      481 ctcaaacata gcctgttttg atgcttccag aatgccagat atcgtaccgg ttaatgcact
      541 caccagcatg cttatcgggg cccctatgag ggatgcactg gaggctgcac taactcccgc
      601 agatacagaa ctcaggacag tatttattgt tgtcagggct gcatctatag ctccggtttc
      661 tttatgaaag gctgcgagta aagcatcccc ttcataattc aattttttaa atcgttcaga
      721 atatgattca agctgcttag ctcgctcaaa tttatctgca gcagccagga aagaaagagg
      781 actgatagcc agcataacag ccgatgtgat cagacccgca cttgcagctg ttgtcgataa
      841 cccctgtgcc attctctgag ccagaatata ttgcgaaaca gctttaccaa catttccaag
      901 aacctgagtt gtcagttcga tacccgctgc agcttttgtt cctgtatgtg cgtcactgtt
      961 tcccagaata aagcttgctg atacagcaga aagaattccg gagacaacat ccagcccatc
     1021 ccccaggggg cccaggtctg gtaaattttg taatttccca ccaacagaac ttaatcgagg
     1081 tttactggat aaaaatgagc caagctggtt aagctgctca gaaaatgaat caacggtact
     1141 gtttatactt gatactgtat ctacaagctg attaataagt tcaatactac ttttggcaat
     1201 atcattctga cttatatcct ctcctgcccg ttgttttctc agcaattcat caagagccat
     1261 gcctgataaa gcaatccccg taaaattctg tagtgctgag agaactgttc cggcttttcc
     1321 cagattatta cctacatttt cagcggttcc tcctatttta ctcccaactt tctgatactt
     1381 ctgcagaagt ttgtcaagtt gtggtgcgaa tattgcaaca ccacgttcag ttaatcctac
     1441 aactttttct gctgcaccaa aaaactggtt tgcaatcgct gtatcatctc gttcagtacg
     1501 gtgtatttct attccaagct catcagc
//
LOCUS       DBFIFI010000403         1526 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.405, whole genome shotgun sequence.
ACCESSION   DBFIFI010000403 DBFIFI010000000
VERSION     DBFIFI010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.405"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..136)
                     /locus_tag="N7B39_004963"
     CDS             complement(<1..136)
                     /locus_tag="N7B39_004963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HGU2424749.1"
                     /translation="MSIKHYDVVRAASPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     Q"
     gene            113..255
                     /locus_tag="N7B39_004964"
                     /pseudo
     CDS             113..255
                     /locus_tag="N7B39_004964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(450..617)
                     /locus_tag="N7B39_004965"
     CDS             complement(450..617)
                     /locus_tag="N7B39_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HGU2424750.1"
                     /translation="MTVKLRLAVAALLLFLVVMVDFTSRIMSVLADGVLVCGIVVLLW
                     PVIKRNSLHNA"
     gene            complement(614..1045)
                     /locus_tag="N7B39_004966"
     CDS             complement(614..1045)
                     /locus_tag="N7B39_004966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance protein"
                     /protein_id="HGU2424751.1"
                     /translation="MFGIFKKKTRRAATEIKKFEKRDLAQVVINAAYLVACADGECEA
                     SEKAKIEQVLRNQPALSAFTSEINAISATIIGQLDTNFKTGRRAALREIEDVKHDTRE
                     AEDVLDVAVAIAEADGEIEPEERKVLEEIAGVLGLRLENHL"
     gene            complement(1215..1291)
                     /locus_tag="N7B39_004967"
     tRNA            complement(1215..1291)
                     /locus_tag="N7B39_004967"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1255..1257),aa:Arg,seq:tct)
     gene            complement(1305..1381)
                     /locus_tag="N7B39_004968"
     tRNA            complement(1305..1381)
                     /locus_tag="N7B39_004968"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1345..1347),aa:Arg,seq:tcg)
     gene            complement(1389..1464)
                     /locus_tag="N7B39_004969"
     tRNA            complement(1389..1464)
                     /locus_tag="N7B39_004969"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1429..1431),aa:Ile,seq:cat)
BASE COUNT          392 a          431 c          363 g          340 t
ORIGIN      
        1 cctgcatcag ggtataaggg gttatggcca ccggactacc aaacggctgc cagccctctt
       61 tcagtttgtg tgtcagcttt tccgcaaggt ctgacggcga cgccgccctg acaacatcat
      121 aatgtttaat cgacatcgaa tttctcccgt gtacaggaac agagttaaaa agccggaacc
      181 ggaatcaaat cacaggatga ccatctgcca gtggctggtc gtaaaaaaaa gccgcgccat
      241 gcgcagccgg aaataaaggg ataacgatga tagtttgaga aaaacagaaa caacactttt
      301 gtggcaaagc atggtgccgg gtgcctcccg gtgaattcag tatcagcacc tgaatccgcg
      361 attatcccat atacctggtt gctgatcgcc cctccgcaca gggggattca ccatgcagaa
      421 gtgtttttaa taaacagcaa acaaaaaaat caagcattat gcaggctgtt tctttttatc
      481 accggccaca gcaataccac aatgccgcag accagcaccc catccgccag caccgacatg
      541 attctgctgg tgaaatccac catcaccacc agaaacagca ggagtgcagc cacagccagg
      601 cgcagtttta ccgtcacagg tgattctcca gacgaagacc cagaacaccg gcaatctctt
      661 ccagcacctt gcgctcttcc ggctcaattt cgccgtctgc ctccgcaatg gccaccgcca
      721 catccagcac atcttccgct tcacgcgtat cgtgtttcac atcctcaatc tcacgtaacg
      781 ccgcacgacg accagtttta aagttcgtat ccagctgacc gataatggtt gcgctaatcg
      841 cattaatttc tgacgtaaac gcggacagcg caggctgatt acgcagtacc tgttcgatct
      901 tcgctttctc ggatgcctca cattcaccat ctgcacaggc caccaggtat gcggcgttaa
      961 tcaccacctg tgccagatcg cgtttttcaa actttttaat ttccgttgcc gctctgcggg
     1021 ttttcttttt gaagattcca aacatcgtga cgttcctttg ggtgggtgag ccaacgcccg
     1081 ggagcgatct gcccacagag aaagtcacac tgaccactcc gtaagctcac ccccgaaagg
     1141 ctctgtggtt ggtatgcgcc gggcgtggcg cagatacaaa aaaggcccgc cgaagcgagc
     1201 ctggaaaata agtgtggcgc gttgtagtgg agtcgaacca ctgaccgatt gcttagaagg
     1261 caattgctct gtcctgctga gctaaaaacg cagaataccg ataatggacc gccatcggag
     1321 actcgaaccc cgcgaaacca gcttcgaagg ctggcgttct atcccgatga gctaatggcg
     1381 gtatgtgatg gtggcccttg ctggatttga accagcggcc tggcgattat gagtcgctcg
     1441 ctctcaccac tgagctaaag ggccgggcgc aggataataa cgttacgaaa tcaatgttgc
     1501 aagcattcaa gaatcacctg gttaaa
//
LOCUS       DBFIFI010000404         1511 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.406, whole genome shotgun sequence.
ACCESSION   DBFIFI010000404 DBFIFI010000000
VERSION     DBFIFI010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.406"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..1007
                     /locus_tag="N7B39_004970"
     CDS             <1..1007
                     /locus_tag="N7B39_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HGU2424752.1"
                     /translation="DLYGKVTALRYFTDDKRDDGDKTYARLGFKGETQINDQMIGFGH
                     WEYDFKGYNDEANGSRDNKTRLAYAGLKISEFGSLDYGRNYGVGYDIGSWTDMLPEFG
                     GDTWSQKDVFMTYRTTGVATYRNYDFFGLIEGLNFAAQYQGKNERTDNSHLYGADYTR
                     ANGDGFGISSTYVYDGFGIGAVYTKSDRTNAQERAAANPLNASGKNAELWATGIKYDA
                     NNIYFAANYAETLNMTTYGDGYISNKAQSFEVVAQYQFDFGLRPSLAYLKSKGIDLGR
                     YGDQDMIEYIDVGATYFFNKNMSTYVDYKINLIDESDFTRAVDIRTDNIVATGITYQF
                     "
     gene            1170..>1511
                     /locus_tag="N7B39_004971"
     CDS             1170..>1511
                     /locus_tag="N7B39_004971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HGU2424753.1"
                     /translation="MIMTNINTACVKNNASYQFNNALPNKETISSNFCERLEQWGNKS
                     LNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDFT
                     DNASLVNINLSF"
BASE COUNT          438 a          319 c          314 g          440 t
ORIGIN      
        1 tcgaccttta tggcaaggtt accgctctac gttattttac tgatgataag cgtgacgatg
       61 gtgataaaac ttatgcccgt ctcggcttta aaggagaaac gcaaatcaat gatcaaatga
      121 ttggttttgg tcactgggaa tatgatttta aaggctataa cgatgaagcc aacggctcgc
      181 gcgacaacaa gacccgtctg gcctatgctg gtttaaaaat tagtgaattt ggctctctgg
      241 actatggccg taactacggt gtcggctatg acattggttc atggactgat atgttgccag
      301 aatttggtgg cgatacctgg agtcagaaag atgtcttcat gacataccgt accaccggtg
      361 tagcaaccta tcgcaactac gatttctttg gcttaattga agggctgaac tttgccgcgc
      421 aatatcaagg caaaaatgaa cgtactgaca acagtcatct ttatggtgct gactacacgc
      481 gtgccaacgg tgacggtttc ggtatctcct caacttatgt ttatgatggc tttggtatcg
      541 gagcggtgta taccaaatcc gatcggacaa atgcgcagga aagagccgct gctaatcctc
      601 tcaatgcctc cggtaagaat gcagaactgt gggctacagg tataaaatat gatgccaaca
      661 acatctactt tgcagctaat tacgctgaaa cattaaacat gaccacctat ggcgatggtt
      721 atatttctaa caaagcacaa agttttgaag tggtggcgca atatcaattc gacttcggct
      781 tgcgcccctc actcgcttac ctgaaatcga aaggcataga tctgggccgc tacggcgatc
      841 aggacatgat tgagtatatc gacgttggtg cgacgtattt cttcaacaaa aatatgtcga
      901 cctatgttga ttataaaatc aacctgattg atgaaagcga ctttacccgt gccgtagata
      961 ttcgcaccga taacatcgtc gctacgggca ttacctatca gttctaacat cctctcctga
     1021 ccagcccttc tctgggctgg tttttgattt aagtgctgct gtatgactcc ttaagtaaaa
     1081 atgagcaatt tttgatattt ctggtcattc acctcaatta atattattcc atacccccgg
     1141 ggacatctta ctataatatt tattgatgag tgattatgac caatattaat acagcttgtg
     1201 taaaaaataa tgccagttat caatttaata acgcattacc taacaaagaa accatctcca
     1261 gcaatttttg tgaacgacta gaacaatggg gtaataagtc gcttaataat ggtgaagaaa
     1321 gagcaattgc cgtggagcga attaaagaag cttacaattc gaacatggca tctcttgatt
     1381 tgtcatatct tgatttgagt gagttaccgc ctatcccctc gacagttaat acgttaaatc
     1441 tggaaaacaa ttgtctaact tgtcttgact ttactgacaa tgccagtctc gttaatatca
     1501 acctcagctt t
//
LOCUS       DBFIFI010000405         1507 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.407, whole genome shotgun sequence.
ACCESSION   DBFIFI010000405 DBFIFI010000000
VERSION     DBFIFI010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.407"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..103
                     /locus_tag="N7B39_004972"
     CDS             <1..103
                     /locus_tag="N7B39_004972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424754.1"
                     /translation="AVKPLKFANQMYIDTGAVFCGNLTLIQVQGEGA"
     gene            100..723
                     /locus_tag="N7B39_004973"
     CDS             100..723
                     /locus_tag="N7B39_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HGU2424755.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAVFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTEQWGIVAEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAITR"
     gene            976..>1507
                     /locus_tag="N7B39_004974"
     CDS             976..>1507
                     /locus_tag="N7B39_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424756.1"
                     /translation="MLSSCIGVVMKDGALLRSSSLFIAYMGCLGWGSAYFYGWGTSFY
                     YGFPWWIVGAGVDDVARSLFFAVIVIAIFLIGWGIGVVFFFAVKRKHSMQELNVFRLY
                     FAVELLFVPAIIEFSILRQKIQVPLLLLSAAIALAVTISIRSYGRFLSVSCFYDKPFI
                     KKHFFEIVMIAFVAYFW"
BASE COUNT          386 a          290 c          378 g          453 t
ORIGIN      
        1 agcagtgaaa ccactcaaat ttgccaacca gatgtatatc gatactggcg cagtgttctg
       61 cggaaacctc acattgattc aggtacaggg agaaggcgca tgagactcga aagcgtagct
      121 aaatttcatt cgccaaaaag cccgatgatg agcgactcac cacgggccac ggcttctgac
      181 tctctttccg gtactgatgt gatggctgct atggggatgg cgcaatcaca agccggattc
      241 ggaatggctg tattctgtgg taagcacgaa ctcagccaga acgacaaaca aaaggctatc
      301 aactatctga tgcaatttgc acacaaggta tcggggaaat accgtggcgt ggcaaagctt
      361 gaaggaaata ctaaggcaaa ggtactgcaa gtgctcgcaa cattcgctta tgcggattat
      421 tgccgtagtg ccgctacgcc gggcgcaaga tgcagagatt gccacggtac aggccgtgcg
      481 gttgatatag ccaaaacaga gcagtggggg atagttgctg agaaagagtg cggaagatgt
      541 aaaggcgtcg gttattcaag aatgccagca agcgccgcat atcgcgctgt gacgatgcta
      601 atcccaaacc ttacccaacc cacctggtca cgcactgtta agccgctgta tgacgctctg
      661 gttgtgcaat gccacaagga agagtcaatc gcagacaaca ttttgaatgc gatcacacgt
      721 tagcgccatg attgccacgg atggcaacat attaacggca taatattgac tttttgaata
      781 actttgggga aacttgacac caataatggg cgttttttac atgtcattga tgagtctcaa
      841 taacctgccg ccgagtagtt tttatgctct gaattgtatt tgtgtagtaa acatgctgac
      901 tgcaatgtaa tagagttttt ttagcctgta acctcttgac ggcattgaat tgcttttgtt
      961 atgagttgta agccaatgtt atcatcttgt attggggtgg ttatgaagga tggtgcgctg
     1021 ctcaggagtt cttcactttt tattgcctac atgggatgcc ttggatgggg gagtgcttat
     1081 ttctatggat ggggtacttc tttttactac ggcttcccat ggtggattgt aggtgcaggt
     1141 gttgatgatg ttgccagaag tttatttttt gcagttatcg tcattgctat atttcttatc
     1201 ggttggggta ttggtgttgt attctttttc gcagtgaaaa gaaaacattc tatgcaagag
     1261 ctaaatgtat ttcgccttta ttttgctgtg gaattattgt ttgtgccggc aattattgag
     1321 ttttctatat tgagacagaa gattcaggta cctcttttgc tactgtcagc agcgattgcg
     1381 ctggcggtta caatttcgat aagatcttat gggcgatttt tatcggtatc atgcttctat
     1441 gataagccat ttataaaaaa acattttttt gagattgtga tgattgcttt tgtggcatat
     1501 ttctggc
//
LOCUS       DBFIFI010000406         1493 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.408, whole genome shotgun sequence.
ACCESSION   DBFIFI010000406 DBFIFI010000000
VERSION     DBFIFI010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1493
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.408"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..587
                     /locus_tag="N7B39_004975"
     CDS             <1..587
                     /locus_tag="N7B39_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311760.1"
                     /note="may be involved in the export or assemby of
                     proteins involved in the entry of Salmonella into host
                     cells; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EscC/YscC/HrcC family type III secretion system
                     outer membrane ring protein"
                     /protein_id="HGU2424757.1"
                     /translation="RPVVLTQENIPAIFDNNRTFYTKLVGERTAELDEVTYGTMISVL
                     PRFAARNQIELLLNIEDGNEINSDKTNVDDLPQVGRTLISTIARVPQGKSLLIGGYTR
                     DTNTYESRKIPILGSIPFIGKLFGYEGTNANNIVRVFLIEPREIDERMMNNANEAAVD
                     ARAITQQMAKNKEINDELLQKWIKTYLNREVVGG"
     gene            592..>1493
                     /gene="sctW"
                     /locus_tag="N7B39_004976"
     CDS             592..>1493
                     /gene="sctW"
                     /locus_tag="N7B39_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA];
                     GO_process: GO:0050709 - negative regulation of protein
                     secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     SctW"
                     /protein_id="HGU2424758.1"
                     /translation="MAIHVEHVGVLERAREVSRLEDIITEDNEDIEAEMPKMRDDPAG
                     KEARFLQATDEMSAALTQFMKKKIYEEQLANFLDGEEYVLEDQPIEKTDKVMEALKAA
                     TTHDYEVYSFAKKLFPDESDLVVVLRAILRKKQISENVRLNAEALLRKVNQETTKKFI
                     NSGINSALKAKLFGQALSLNPKLLRASYRQFLMAEDDAVDTYVEWIGSYGYQNRMLVT
                     KFIKETLFSDINALDASCSSLEFGMFLNKLSQLLSLQSAEALFLKTLMNNPIIKKFIS
                     AEDYWIFFLISLIKFPETAEELLN"
BASE COUNT          514 a          248 c          323 g          408 t
ORIGIN      
        1 ctcgtcccgt cgttttaaca caagagaata ttcctgctat atttgataat aaccgcactt
       61 tctatactaa attagtcggc gagcgtactg cggaattgga tgaagtaacc tatggtacga
      121 tgattagcgt gctaccacga tttgctgcgc gaaatcaaat tgaattactc ttgaatattg
      181 aagatggaaa tgaaatcaat tccgacaaga ccaatgttga tgatctgcct caggttggta
      241 gaacacttat aagtactatt gcacgcgtgc cccaagggaa aagtcttttg attgggggat
      301 atacacgcga tacgaatacc tatgaaagtc gcaagatccc aatattaggc agcattccat
      361 ttattggtaa attatttggt tatgaaggaa caaatgcgaa taacatcgtt cgtgtctttc
      421 ttattgagcc aagagagatc gatgaacgca tgatgaataa tgcgaatgag gctgcggttg
      481 atgccagagc gattacacag caaatggcaa aaaataaaga aatcaacgat gaattactgc
      541 agaaatggat aaaaacttac ctgaatcgtg aagtcgtcgg aggatagtga aatggcgatt
      601 catgtagagc atgtcggtgt attagaaaga gccagggaag tttctcgctt agaggacatt
      661 atcacagaag ataatgaaga cattgaagct gaaatgccta aaatgaggga tgatcccgca
      721 ggtaaagaag ctcggttctt acaagctact gacgagatgt cagcagcgtt aacgcagttt
      781 atgaagaaaa aaatttacga agaacaactg gccaattttc tggacggaga agaatatgtc
      841 ctggaagacc agccaataga aaaaaccgac aaagttatgg aggcgcttaa ggctgcaact
      901 acccatgact atgaagtgta tagctttgct aaaaaattat tcccagatga aagcgatctg
      961 gtagttgtgt tacgtgcaat tttacgtaaa aaacaaatat cagaaaatgt ccggctaaac
     1021 gctgaagcat tacttagaaa agttaatcaa gagactacta aaaaattcat taatagcgga
     1081 attaactcgg cattaaaagc aaagttattt ggccaggcgc tgtcattaaa tccaaaatta
     1141 ctaagggcga gttatcggca atttttaatg gcagaagacg atgctgtaga tacttatgtc
     1201 gaatggattg gtagttatgg ctatcaaaat agaatgttgg tgacaaaatt tatcaaagag
     1261 acgcttttct cagatataaa tgcacttgat gcgagttgtt catcattgga atttggtatg
     1321 tttctcaata agttgtcaca actattgtct cttcagtcgg cagaggcttt atttctgaaa
     1381 actttaatga ataacccaat aattaaaaaa tttatcagcg cagaagatta ctggatattt
     1441 tttctgatat cgttaataaa atttcctgag actgcagagg aactcctgaa caa
//
LOCUS       DBFIFI010000407         1452 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.409, whole genome shotgun sequence.
ACCESSION   DBFIFI010000407 DBFIFI010000000
VERSION     DBFIFI010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.409"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            150..1206
                     /locus_tag="N7B39_004977"
                     /pseudo
     CDS             150..1206
                     /locus_tag="N7B39_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          420 a          352 c          322 g          358 t
ORIGIN      
        1 tgtaggatta ttttaatcta atttataagc ataaaatata tactcactaa atagctgcgc
       61 agaatagtag atcatttaga gggaactcaa cccgattgtg cgatctgatc aatcgccaaa
      121 tcaatacaaa tcaccaaccg gactgagcaa tgtcgatcat accaccaatt tcccgtgacg
      181 aacgacgcct aatacagaaa gccatccata aaaggctgac agccatgctg atgctgcacc
      241 ggggcgaccg tgtcagcgac gttgcccgca cactatgctg cgcccgttcc tctgttgggc
      301 gctggattaa ctagttcaca cagtcgggtg ttgagggact gaaatcatta cctgccgagc
      361 gagcccgccg ctggccgttt gcgcatatct acacactatt acgtgaacag gtaaaatatt
      421 cactcgcgac tttggctaca attattcacg ctggagtaca gaactgctga caataaaaat
      481 caatgagata accggctgcc tgttacatgc tggaaccatt cgccgctggt tgtcgtctgt
      541 ggggcttgtg tggcgaaggg ctgcgccaac tctacgtatc cgtgccccgc ctaaagataa
      601 aaagatggca acaatccata acgcactgga cgaatgcagc acagagcatc cggtctttta
      661 tgaagatgaa gtatttatcc atcttaatcc caaaatcggt gcggactgga aactgctcgg
      721 gaagcaaaaa cgaggggtca cgccggaaca aaatgaaaaa tattctctgg acgtagcgct
      781 gcacagcggg acaggttaag tcagctgtgt gggcgccaac agcaaaagtt cggcgctgtt
      841 catcagactg ctgaagcggc ttaaagctac atactgtcgg acgaaaacca tcacgctgct
      901 cgaggacaac tacattatcc acaaaggccg ggaaacccag cgctggctga aggataaacc
      961 gaagtgcaga gtcatttatc agccggttta atctccatgg gtgaatcatt ttgaatagct
     1021 atggcagata cttcacgcca caataacgcg taatcatcag tgccgctcaa tgtggcaact
     1081 attgaaaaag ttcgccattt tatggaatcc gtcagtccat tccccgaagg caaacatggg
     1141 ctggcaaaaa tgtgcgatat taggcgcagc tatttagtta ttccgtcatt tctcccgccc
     1201 gtgtaaacac tactgaatct tgtcgttgtg acaaagctat aaatagtcaa tcacagcacg
     1261 tttctgcgta cgacgcgctg caattttctt tacgatttat aactgtacag gtgaactatg
     1321 agttatttgg atacatacat atagtgtaac gcctgttgta tattttactc atatcttttt
     1381 acgggatgaa aaccaatgat actggcgcat agcctcctcc aaccttaccc ctgtataaca
     1441 cgactcatat aa
//
LOCUS       DBFIFI010000408         1449 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.410, whole genome shotgun sequence.
ACCESSION   DBFIFI010000408 DBFIFI010000000
VERSION     DBFIFI010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1449
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.410"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            134..376
                     /locus_tag="N7B39_004978"
     CDS             134..376
                     /locus_tag="N7B39_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HGU2424759.1"
                     /translation="MYELTLSPAEIKEITKYERYTKQQHQLRLHGIPFVIGPKNEPIV
                     LRRDIPHGLTTMPKTSELVSAEPDFEALNNGKTKKK"
     gene            354..1360
                     /locus_tag="N7B39_004979"
                     /pseudo
     CDS             354..1360
                     /locus_tag="N7B39_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309082.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          481 a          309 c          337 g          322 t
ORIGIN      
        1 cgttaccagg cgaaatacgt ttcagccatc tggcaaaaac tgctcccgag tcaggctgat
       61 ttttgatgtt caacccatat cgccgcccac cagctatagt ggcggcggtc atgctgtaaa
      121 ggcacgtgac cacatgtacg aattaactct atctccagca gagattaaag agatcacgaa
      181 atacgagcga tacacaaaac agcaacacca gttaagactg cacggcatcc catttgtaat
      241 cggccctaaa aacgaaccca tagttctccg cagggatatt ccacacggtc tgacaacgat
      301 gccaaaaaca tctgaactgg tttctgctga acccgatttt gaggcgctga acaatgggaa
      361 gaccaagaaa aaataaaaaa gataatgtac tgccaccgcg ggttagatcg aatggttaca
      421 gttacgtgtg gaaacccgaa ggaagtacaa gaagtatagg gctaggaaga gtgcggaaaa
      481 ccagcgtagc taaagtctgg caaaattatg aactggaaaa agcaaaactc cacaacataa
      541 tgaccgtagc taaattgtgg cacatgttta tggactcccc tgcatttaca gaactggccc
      601 cccgaaccca aaaagattat cgacaacatc agaaggcgtt gctgatggta ttcggaaaag
      661 tgcttgctga taatgtcaaa actgagcagg taagaatttt catggataaa cgagggcttg
      721 ggcaaatcat gaactggcaa gcctgagtcg agtatacggg tggggatatg agcgtgggta
      781 tgtgaagaat aacccatgca aaggagtcag aaaattctct cttaaagccc gcactgttta
      841 catcaccgat gaacagtatg cggcgatata tgcggaagca attccacagt tacgcattgc
      901 aatggagatt tcctatctct gtgcggcaag actcggtgat gtgcttgagt tgaaatggca
      961 ggatattatg gataaaggga tctacattga gcaaaacaaa accggcacca aacaaatcaa
     1021 ggaatggtca ccgcgattac gtacagcgat ccagttagcc cgaaatgtat cttcctgtac
     1081 atgcgaatat gtgatcaata caaccaaagg cgggaaagtc atagctaaaa cgctgaataa
     1141 ctggtggaat caggctaaac gcgcagccga gcaaaaagtt ggcgttccgt tcgggtgcaa
     1201 ttttcacgac ataaaagcca aggggatctc agattacgaa ggcagcagtc gcgacaaaca
     1261 aattttcagc gggcataaaa cagaaaatca ggtgttgatt tacgatcgta aaacaaaaat
     1321 cacaccaaca ctggatttgc cgctcgtggt tagcaagtag acaatccaga aaaatttctg
     1381 gcaaaaagac ttggtatact agctatgagc aacttcctaa taccttgatt tttcttggtg
     1441 ttaaatttg
//
LOCUS       DBFIFI010000409         1447 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.411, whole genome shotgun sequence.
ACCESSION   DBFIFI010000409 DBFIFI010000000
VERSION     DBFIFI010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.411"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            128..409
                     /locus_tag="N7B39_004980"
     CDS             128..409
                     /locus_tag="N7B39_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HGU2424760.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLLEKLKHAAEKIRSQFA"
     gene            375..>1447
                     /locus_tag="N7B39_004981"
     CDS             375..>1447
                     /locus_tag="N7B39_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU2424761.1"
                     /translation="MQRKKYDPNLPKNLTYRRRDKAYYWRNPLTKEEFTLGKISRRDA
                     IAQAIEANHYIYKNYSTAALIEKLKGFDSFTMADWIERYKTILIRRKVSRNTYKIRVN
                     QLETIKEKLGEILLTEITTRHIAEFLDLWIEGGKNTMAGSMRSVLSDMFREAIVEGRI
                     SQNPVTPTRAPKIVVTRERLKLKTYNCIREAADQLPAWFPLAMDLALVTGQRREDITN
                     MRFSDIYDDRLHIRQIKTGMMIAIPLSLSLPVAGLRLGTVVEQCRLVSRGDYLISAGI
                     RKNSPDGSIHPDGLTKKFVAARKLTGIQFSENPPTFHEIRSLAGRLYKETCGEEFAQR
                     LLGHTSEKTTKMYLDEREKTYLLL"
BASE COUNT          497 a          293 c          316 g          341 t
ORIGIN      
        1 gagaaacttc tgtcctcgtg agttgcatta tatacaaatt agaacttcat agctgattat
       61 taaaaatcaa ccacacccgc cagtattctg tatatttact ggcggtcata tcgtaagagg
      121 tatggcaatg aatcttgtga cactcaaaac gtggggaaaa ctcagatatc cggataaccc
      181 accatcaata tcaacgctga gacgatgggc aaggaatgga aacatttatc ctgcacctga
      241 actacacggg aggagttaca gggtggttcc ggaagctttc tatatcaacc ccaataaggt
      301 tgataccgat ataacacacc atcagcctaa tgggcgacaa gggagagaca gtccgttact
      361 ggagaagtta aaacatgcag cggaaaaaat acgatcccaa tttgcctaaa aacttaacat
      421 atcgaaggag ggacaaagca tattactggc gcaaccctct gacgaaagaa gaatttacac
      481 taggtaaaat ttcaagaaga gatgcaatcg cgcaggcaat tgaagcaaat cactatatat
      541 acaaaaacta ctctactgct gccttaattg aaaaacttaa agggttcgac tcatttacta
      601 tggcagactg gattgaacgt tacaaaacga ttcttataag gagaaaagtg tccagaaata
      661 cttataaaat tcgggtaaat caattggaga caataaaaga aaaattggga gagattttac
      721 tgacagaaat aaccactcgc catattgccg agtttcttga tttgtggatt gaaggaggga
      781 aaaacacaat ggcaggatca atgcgttctg tgttgtctga tatgtttcgt gaggccattg
      841 ttgaaggacg tatatctcaa aatccagtaa cgccaacaag agcaccgaaa atagtagtta
      901 caagagaacg actgaaacta aagacataca actgcatcag ggaggcagca gatcaacttc
      961 cggcatggtt cccattagct atggatttag cccttgtaac aggacaacgt cgcgaagaca
     1021 taacgaatat gcggtttagt gatatttatg atgatcgtct ccacatcagg caaattaaga
     1081 caggaatgat gattgctatc cccctgtcac tcagccttcc tgtcgctggt ctacggcttg
     1141 gtacagtagt tgaacagtgc cgcctggtaa gccggggaga ttatctaatc agtgccggga
     1201 ttagaaaaaa cagccctgac ggcagcattc acccggatgg cctgacaaaa aaatttgtcg
     1261 cagccagaaa attaacaggt atccagttca gtgaaaaccc accaactttt cacgagatca
     1321 gaagcctggc tggacgattg tacaaagaaa catgtggaga agaatttgct cagcgtctac
     1381 ttggccacac atcggagaag acaacaaaaa tgtatcttga tgagagagaa aaaacgtact
     1441 tactgct
//
LOCUS       DBFIFI010000410         1422 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.412, whole genome shotgun sequence.
ACCESSION   DBFIFI010000410 DBFIFI010000000
VERSION     DBFIFI010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.412"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..1322
                     /locus_tag="N7B39_004982"
     CDS             <1..1322
                     /locus_tag="N7B39_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HGU2424762.1"
                     /translation="FDRFIKRFPIIGSSTHSIINSIGKGALLDYVIIDEASQQDIVPG
                     ILGLGCARNVIVVGDRKQLPHVPVLLPNSPSPPAEYYNCEKYSLLDSVCMLFRNMVPV
                     TLLKEHYRCHPKIIQFCNKQFYDNALIPLTVDSGEASLSLVITAKGNHTRNFSNLREL
                     ESLEGHYWDEESSRGYIAPYNAQVNLAEKVLPADFVKSTVHKFQGRECDEIVFSTVLD
                     KKRSSQHSRNIAFVDNPELVNVAVSRARNKFTLVTGNDVFERHAGHIAALIRYIKYYA
                     DDGEIFESPVISAFDLLYSEYDKSLERLNSRLNSNDSHFKSEQIVACLLRDILSQDSY
                     RSMMFHSQIALNQLVLLERGDFTHREQLFMRNRASCDFVVYYKVGKTPLGVIEVDGGY
                     HLTSVQAERDELKNSILKKCGLPLLRLRTIDSDIEGKLGAFLSGLTG"
BASE COUNT          387 a          268 c          350 g          417 t
ORIGIN      
        1 aatttgaccg ttttataaag cgttttccga ttattggtag cagtacgcat tcgattatta
       61 actctatcgg caagggggcc cttcttgatt atgttattat tgatgaagcc tcgcagcagg
      121 atattgttcc gggcattctg ggactgggat gtgcccgtaa tgtaatcgtt gtaggggatc
      181 gtaagcaact tccacatgtt cctgtattac tcccgaacag cccatctcca ccggcagaat
      241 actataattg tgagaaatat agtctacttg attcggtttg tatgttgttc aggaacatgg
      301 tgccagtaac ccttcttaaa gagcattatc gttgtcatcc taaaatcatt caattttgta
      361 ataagcagtt ttatgataat gctctgatac cgttaacggt tgattccggt gaagcctcat
      421 tatctctggt catcacggca aaaggaaacc atacccgtaa tttttcaaat ctcagggagc
      481 ttgaatctct ggaaggtcat tactgggatg aagaaagtag caggggatac attgccccat
      541 ataatgcaca ggttaatctg gctgaaaaag tgctgcctgc tgattttgtg aaatcgacgg
      601 tgcataaatt tcagggacgg gagtgcgatg aaattgtgtt ttccactgtg ctggataaga
      661 agcgcagcag ccagcacagt cggaacatcg cgtttgttga taatcctgaa ctggtgaatg
      721 tcgctgtttc aagggcccga aataaattta cgctggtgac tggtaatgat gtatttgaac
      781 gccacgctgg acatattgcg gcgctgattc gttacatcaa atactacgct gatgacggag
      841 agattttcga gagcccggtc atatcagcat ttgatttgct gtacagtgag tatgacaaat
      901 cactggaacg actgaatagt agattgaatt caaatgattc acactttaag tcagaacaga
      961 ttgtggcctg cctgttgcgg gatatcctgt cgcaggatag ttatcggagt atgatgtttc
     1021 attcacaaat agcgctgaat cagttggtgt tgctggagcg gggggatttt acacaccgtg
     1081 aacagttatt catgcgtaac cgggccagtt gtgactttgt tgtttactat aaggtcggga
     1141 agactccatt aggggttatt gaagtggatg gaggttatca tctgaccagt gttcaggctg
     1201 aacgtgatga acttaaaaac agtatactga agaaatgcgg cctgccattg ctaagattac
     1261 gtactataga cagtgatata gaagggaagt tgggagcgtt tctgtcaggc ctgacaggct
     1321 aaaatctgaa taaaaaatat tgcagcggga ggtctcaaac ctgatttggt catgtgccac
     1381 acacaattgt acgaacccca tggacacagg cttctttgtt tt
//
LOCUS       DBFIFI010000411         1375 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.413, whole genome shotgun sequence.
ACCESSION   DBFIFI010000411 DBFIFI010000000
VERSION     DBFIFI010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.413"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..839
                     /locus_tag="N7B39_004983"
     CDS             <1..839
                     /locus_tag="N7B39_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1524 domain-containing protein"
                     /protein_id="HGU2424763.1"
                     /translation="FKRELKVRDLYNFRSRSYWLRRLENDKRRERVEEFTIEHIMPQN
                     ENLSAKWREELGSDWQRIHKELLHTLGNLTLTRYNSRYSDRPFAEKRDIEDGFKHSPL
                     YLNIGLGQCEKWDEAAIHARADRLAELAVQVWQAPSLPEEVLAVYRGQPENKTSYSLS
                     DYPFLADGSHSRVLFDHLRDEIMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLRL
                     SLNMQFHELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMESA
                     LV"
     gene            complement(886..1227)
                     /gene="symE"
                     /locus_tag="N7B39_004984"
     CDS             complement(886..1227)
                     /gene="symE"
                     /locus_tag="N7B39_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HGU2424764.1"
                     /translation="MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTVVDVKVMEGCIVLTAQPPAAAESELMQSLRQVCKLSARKQRQVQEF
                     IGVIAGKQKVA"
BASE COUNT          361 a          301 c          362 g          351 t
ORIGIN      
        1 aatttaaacg ggaattaaaa gttcgcgatc tctataactt ccgtagtcgt agctactggt
       61 tacgacgact ggaaaacgat aaacgcagag agcgcgtgga agagtttacg attgaacata
      121 ttatgccgca gaacgaaaat ttgtcggcta aatggcgcga agagctggga agtgactggc
      181 agcgtattca taaagaactg ttgcatacgt tggggaatct cactttaacg cgctataact
      241 cccgttacag tgacagacct ttcgcagaaa aacgcgatat tgaagacggc tttaagcata
      301 gcccgctcta tttgaatatc ggtcttggac agtgcgaaaa atgggatgaa gctgccattc
      361 acgcccgagc cgatcgtctg gcggagcttg cggttcaggt ctggcaagcg ccttctcttc
      421 ctgaagaggt tttagctgtt tatcggggac agcctgagaa caaaaccagt tacagcctga
      481 gtgattatcc ttttcttgct gatggttcgc atagccgggt gttattcgat catcttcgcg
      541 atgaaattat gcgcctggac gcagggatca cgcaggaagt attgaagctc tatattgcgt
      601 ttaaagcgga aacgaatttt gttgatgttg tgccgcaaaa aagccgactg cgattgtcgc
      661 ttaatatgca gtttcatgaa ctggtcgatc cgaaaggtat tgccaaagat gtgacaaatg
      721 ttgggcgatg gggcaatggt gatgtggaaa ttggtttcag tgacctcgca caacttcctt
      781 acattatggg gttaattcgt caggcatttg aaaaacagat ggagagcgcg ttggtataac
      841 gcagcgaaaa atgcccggta gtcctggcgc taccgggtca atgtatcagg caactttctg
      901 tttacccgca atcaccccaa taaactcctg cacctgcctt tgtttacgcg ccgacagctt
      961 gcacacctgg cgcagcgact gcatcagttc gctctcggcg gcggcgggtg gttgggcggt
     1021 gaggacaata cagccttcca tcactttgac atctaccacc gtgccggtgg caaaaccggc
     1081 ggcttccagc cactgacctt tcagggtgat ggcgggaata cggctgtaat ccgggtaacg
     1141 actcgcataa ctcacggtta attgacggtt atttgccggg gagacttctg cttcgaacgg
     1201 ttgtgcaata gaatgcgtat cagtcataac tgctattccc tataaatagt gattgtgatt
     1261 agcggtgcgg gtgtgttggc gcacatccgc accgcgctaa atacctggat atatcatcag
     1321 taaatatggg gaaagtccag ctaaaaatta atgaaaataa gatgaaaatt agcgt
//
LOCUS       DBFIFI010000412         1373 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.414, whole genome shotgun sequence.
ACCESSION   DBFIFI010000412 DBFIFI010000000
VERSION     DBFIFI010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.414"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            85..348
                     /locus_tag="N7B39_004985"
     CDS             85..348
                     /locus_tag="N7B39_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HGU2424765.1"
                     /translation="MKPNIHPEYRTVVFHDTSIDEYFRIGSTIKTDRVIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVDAKKGG"
     gene            650..790
                     /locus_tag="N7B39_004986"
     CDS             650..790
                     /locus_tag="N7B39_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HGU2424766.1"
                     /translation="MPQKTIIVGMLCLTMLLTVWVLHASPCEFRVSFMWSEIAAFLQC
                     KP"
BASE COUNT          374 a          290 c          337 g          372 t
ORIGIN      
        1 gaggttataa gaacagacag ttgaatctga ccgtctgaat atgtaccttt atgcggaatt
       61 ttcacttaaa gggggaaaac tgttgtgaaa ccgaatatcc atccggaata ccgtacagta
      121 gtatttcacg ataccagtat tgatgagtac ttcaggatcg gttcgacgat caaaactgac
      181 cgggtaattg aactggatgg tgtaacttac ccatatgtta caattgacgt ttcttctaag
      241 tcgcatccgt tttatacagg taaactgaga actgtcgctt cagaaggtaa tgttgcacgc
      301 tttacccagc gttttgggcg ttttgttgat gcgaaaaaag gtggctgatt ttgccgccag
      361 ggtgcgtttg acaatttaaa aacgcaccag acagagagat cctgcacctg aatataccgt
      421 tagcccgtcc caccggggcg tgtgcagatg atctcgtcac cggaagattg cggacctctc
      481 tgaaaaccag tacattacgc gcgggtgcct ttcggctgac ggctggaagg attacctgaa
      541 ggccggatgt ggaaaggccc cgagtcaact taaacgttaa cccgaggccc tgaccatcta
      601 ccttcagcaa gtggaaggtt agcgcctctc tgtaaaagga gcaagtgcta tgccgcaaaa
      661 aacgatcatt gtgggcatgt tatgcctgac catgttgctg accgtctggg tgttacacgc
      721 atcaccgtgt gaattcaggg taagcttcat gtggtcggaa attgcggcgt tcttgcagtg
      781 taaaccgtaa gaaaccgcag cggggacaac agtccccgct atccggttgc caaaggttcg
      841 gtcgaaagtc gcacccttgc acacaaatca caaagaaaaa acaaaagtcc gcagtgtaaa
      901 aactgtgggc ttttttattc gcatcccgaa agtgtggtaa cagcgtgggg gcaccaaaaa
      961 aagccccata aaatggggct gaagattctg taaggaattt cacagcaatg agattcctgc
     1021 tttctccaag gatatacgtg tcttcaggct gaatcaatgg gggcgtggct ctgaatccac
     1081 ctgcgattaa aacttgcaca atgccgggag ttgagttaga aaagtacagg gcgcgatatc
     1141 cggcttattt tgttgctgtg ttttgcctcg tgctggatgt cgcgtccacc gtttttctga
     1201 tgattcatat gaaatggtac tttattgatt tactgccatt ctgatttgcc tttttatgtt
     1261 cattattgcg gttgtcactt ccagtttctg gtgatgtttt gataaaagtg aaagaataaa
     1321 acgataattg cagaaggtat tgctaaaact atacatatca ttatataaac aag
//
LOCUS       DBFIFI010000413         1370 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.415, whole genome shotgun sequence.
ACCESSION   DBFIFI010000413 DBFIFI010000000
VERSION     DBFIFI010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.415"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..>1370
                     /locus_tag="N7B39_004987"
     CDS             <1..>1370
                     /locus_tag="N7B39_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HGU2424767.1"
                     /translation="GAATVNLINGSGKPVSVAITAHPAPDRIQVSTLPDGVETYGVWG
                     LSLPSLRRRLFRCVSIRENTDGTFAITAVQHVPEKEAIVDNGAHFDGDQSGTLNSVIP
                     PAVQHLTVEVSAADGQYLAQAKWDTPRVVKGVRFSLRLTSGSGEGSRLVTTAITADTE
                     HRSSGLPPGEYTLTVRAINSYGQQGEPATTTFRINAPAKPATIELTPGYFQITAVPRL
                     AVYDPTVQFEFWFSETKIADTSQVETSARYLGTGSQWSVSGPHIKPGKDFWFYVRSVN
                     LVGKSAFVEVSGQPSNDGEGYLEFFREKIGKLHLAQGLWELIDNSQLADEMAEMKTTI
                     TETRNEITQTVSKTLEDQSATIQQIQRVQKDTNDDLAALYMLKVQKTKDGIPYVAGIG
                     AGIEDTDGQPLSNILLLADRIAMINPENGNSTPLFVAQGNQLFMNDVFLKRLFAVSIT
                     SSGNPPA"
BASE COUNT          326 a          370 c          422 g          252 t
ORIGIN      
        1 ggtgccgcca cggtgaacct gattaacggc agcggtaagc cggtgagcgt ggccatcact
       61 gcacaccccg cgccggaccg gatacaggtc agcaccctgc ctgatggtgt ggagacatac
      121 ggtgtatggg gactctccct gccgtcactg cgtcgtcgcc tgttccgctg tgtctccatc
      181 cgggaaaaca cggacggcac ctttgccatc acggcggtgc agcacgtacc ggaaaaagaa
      241 gccatcgtgg ataacggagc gcactttgac ggcgaccaga gcggcaccct gaacagcgtt
      301 attccaccgg cagtgcagca cctcacggtg gaggtgagcg cggctgacgg ccagtatctg
      361 gcacaggcga aatgggacac gccgcgggtg gtgaaggggg tgcgcttcag tctgcgactg
      421 accagcggaa gcggagaagg cagccgtctg gtgaccaccg ccatcaccgc ggatacagag
      481 catcgttcca gtggtctgcc gcccggggaa tacaccctga cagtcagggc aattaacagc
      541 tatggccagc agggcgaacc ggccaccacc accttccgga ttaacgcgcc agcaaaaccc
      601 gccaccattg aactgacgcc ggggtatttt cagataacgg cggtcccgcg tcttgcggtg
      661 tatgacccga cggtacagtt tgagttctgg ttttcggaga caaaaatcgc agacacatct
      721 caggtggaaa cctctgcccg ttatctgggg accggcagtc agtggagtgt atccggcccg
      781 cacattaagc ccgggaagga tttctggttt tacgtgcgca gcgtcaacct ggtggggaaa
      841 tctgcgtttg tggaagtcag cgggcagccc agcaatgatg gtgaagggta tctggaattt
      901 ttccgggaaa aaataggaaa actgcatctg gctcaggggc tgtgggagct gatagacaac
      961 agccagcttg cggatgagat ggcggagatg aagaccacca tcaccgaaac ccgcaatgaa
     1021 atcacacaga cggtcagtaa aacgctggaa gaccagagcg ccaccattca gcagatacag
     1081 cgcgtgcaga aggacacaaa tgatgacctg gctgcgctgt acatgctgaa ggttcaaaaa
     1141 acgaaagacg gcattcccta tgtggccggg attggtgcag ggattgagga tactgatggc
     1201 cagccactga gcaacatact gctgctggct gaccgtatcg cgatgataaa tccggagaac
     1261 ggcaacagca cgccgttatt tgtggcgcag gggaatcagt tgttcatgaa cgatgtgttc
     1321 ctgaagcggc tgtttgcggt gagtatcacg tcatccggca atcctccggc
//
LOCUS       DBFIFI010000414         1369 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.416, whole genome shotgun sequence.
ACCESSION   DBFIFI010000414 DBFIFI010000000
VERSION     DBFIFI010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.416"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..654)
                     /locus_tag="N7B39_004988"
     CDS             complement(<1..654)
                     /locus_tag="N7B39_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HGU2424768.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTTCHGVSLRGHIDVTTEDLASALDVTEDVIDSILHAMQGRVLDGDLISGWEKRQV
                     MKEDNGNVSQTAKSPAERKRAQRERERLRKQNTDCHDESRRVTHMSRQITTDTDTDKE
                     LNPTHNARMRESATTGELNDASLQTAEPEYLDGLSEPIGKFPMTGVWQPSPDFRQRAA
                     "
     gene            complement(723..1148)
                     /locus_tag="N7B39_004989"
     CDS             complement(723..1148)
                     /locus_tag="N7B39_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HGU2424769.1"
                     /translation="MKIKHEHIRMAINAWAYPDGEKVPAAEIARTYFELGMTFPELYD
                     DSHPEALARNTQKIFRWLDKDTPDAVEKIQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVAAAIAWGTLTNSGGQPGNAVVVH"
     gene            complement(1145..>1369)
                     /locus_tag="N7B39_004990"
     CDS             complement(1145..>1369)
                     /locus_tag="N7B39_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HGU2424770.1"
                     /translation="RVDELVQFFGSVQRVADFYGITREAIYMWRKRPGEIVPKGRAAE
                     AAAYSKGKLSLNPELYKKKDTTQGEGKGDS"
BASE COUNT          301 a          397 c          309 g          362 t
ORIGIN      
        1 tgctgcccgc tgtcgaaaat ccggcgacgg ttgccagact ccagtcatcg gaaatttccc
       61 gatcggttcg ctcaggccgt ccaggtattc aggttcggct gtctgcaacg acgcgtcatt
      121 caactcaccg gtcgtagcac tctcgcgcat gcgcgcgtta tgtgtggggt ttaattcttt
      181 atctgtatct gtatctgtcg tgatttgtcg tgacatatgc gtgacgcgtc gtgactcatc
      241 gtgacaatca gtgttctgct tccgcagtct ttcccgctcc cgctgcgctc tcttgcgctc
      301 tgccggggat tttgcggttt gcgaaacatt accgttatcc tccttcatca cctggcgttt
      361 ttcccatccg gaaataagat caccatccag aactcgcccc tgcattgcat gcaaaattga
      421 atcaatcacg tcttccgtca catcaagcgc acttgctaaa tcttccgtcg tgacatcaat
      481 gtgaccacgt agtgacacgc cgtgacatgt cgtgacattt cgtgacgcac tcaccagaag
      541 gtggatatac accgccatca ctgttgcgat tggctgtcct gagaccctgg caatggttcg
      601 ccatttggga tcatttggca tgtcatgcca caatctgagc caggcattag ccatactcac
      661 ctcatctgat accgaacttt accctcgaac atccggaaga aatccggcat gaatattgtt
      721 ggtcaatgca cgacaacagc attaccaggc tgaccaccac tgttagtcag ggtgccccag
      781 gcgatcgccg ctgcgacaaa atcatccaca tctttcacca gccgatccct ccgttcgacg
      841 atctcacggt aatattcaga gctgtgactg cgcatacggg ccaccagcag aggcggcatt
      901 gccttttcga tcgccggtaa cagagcctga attttttcaa cagcatcagg ggtgtcttta
      961 tccagccaac ggaaaatttt ctgggtatta cgagccaggg cttccggatg gctgtcgtcg
     1021 tacagttccg ggaacgtcat ccccagctcg aaataagtcc gggctatttc agctgcagga
     1081 actttctcac cgtccggata ggcccaggca ttgatcgcca tgcggatgtg ctcatgtttg
     1141 attttcatga atcccccttt ccttcgccct gagtggtatc cttctttttg taaagttctg
     1201 ggttcaaaga taatttcccc ttggagtatg cagcagcttc cgcagccctc cccttcggaa
     1261 ctatttcacc aggacgctta cgccacatgt aaatagcttc gcgggttatc ccataaaaat
     1321 cggcaaccct ctgaacagaa ccaaaaaact ggacaagttc atcaactcg
//
LOCUS       DBFIFI010000415         1367 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.417, whole genome shotgun sequence.
ACCESSION   DBFIFI010000415 DBFIFI010000000
VERSION     DBFIFI010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.417"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..84)
                     /locus_tag="N7B39_004991"
     CDS             complement(<1..84)
                     /locus_tag="N7B39_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HGU2424771.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAH"
     gene            complement(488..1249)
                     /gene="ydeO"
                     /locus_tag="N7B39_004992"
     CDS             complement(488..1249)
                     /gene="ydeO"
                     /locus_tag="N7B39_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HGU2424772.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTMPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFATCKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMNALAI"
BASE COUNT          444 a          243 c          241 g          439 t
ORIGIN      
        1 atgagcagca aatgcagcca taccagatgc cagtatcaaa gatatcgagg tacttacgac
       61 acttttcttt aatgcagact tcataaatgt tcctcttctt attgtactta attccaaagt
      121 aattttacga agagaaataa gtggatgtaa gtgaagttag tcacataaag agatagcaga
      181 tttagctaaa aaaagggaaa taacagtcca taaagcgttg acattacttt ttgttctact
      241 aagtaatttc tcgacgataa acaattaatc tattgatatt taataaatta ttgtatttta
      301 ctaacaaaat gccgaattga gatcataaat aatcatgcaa caagttatgc aaatgcataa
      361 atatgtgatg aatgtcactt atttatttca ataaatatat cgcctaaaaa caacgcgggg
      421 cagggaatga ctgccccatt tttaattttt acgtagggtg cgtggttgac tactcgttag
      481 caaataatca aatagctaaa gcattcatcg tgttgcccgt attcataccc gtgtgccttt
      541 gacaacggta ctccttagaa actctctttg gactgttgcc gaaatgtttg cggaacgcat
      601 aaataaaata agatgtactg gcataaccac attgttcggc aattttattg actgaacctt
      661 ctacgcgtat caaatttttt gcgtgctgca ttctggcatc taaaagaatc tgtgagaatg
      721 tcgtttgctc ttgctttagt tttttcttca acaggctttc actgatgtac aggcagtcac
      781 aaatatcttt cagcttccat gggtgcgcca gcttcatgtt gacaatattt ctcactttcc
      841 ccgaaacgga tagcacaccg ttagttaata gtgtaatgaa acctttgcat gtggcaaaaa
      901 tagacaggca agaaaaaagc aaaagttctg aaaaattatg atgattgcgt tcttccgagt
      961 tgagataagc aatcatttca ttaagcaatc cagtcggtac attcgaagca cgtaaaaact
     1021 tcgcaggaac aggcatcgtc actgtctgga tgttagtgca ttgtagatat tttttaatgg
     1081 tattttcatt aaatccgatt tctttaaaat gccgctcata aggttcaaaa tgcgtacgaa
     1141 cagcgttatc taccatcagg atctcgccgt cagagaaggc gtaatcttta tctaaaatgt
     1201 tagcgttgaa ggcatgatga ataaatataa cagaacaaac gagcgacatt ttatctcctt
     1261 aaaacaataa agttttatcg atactttttt aacatttcat atttataatt tgctgtttat
     1321 tttcagtctt gcaaactatt gacaatgaaa tgtgtaagat ctcttgc
//
LOCUS       DBFIFI010000416         1361 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.418, whole genome shotgun sequence.
ACCESSION   DBFIFI010000416 DBFIFI010000000
VERSION     DBFIFI010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.418"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            456..>1361
                     /locus_tag="N7B39_004993"
     CDS             456..>1361
                     /locus_tag="N7B39_004993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HGU2424773.1"
                     /translation="MAFKHYDVVRAASPSDLAKRITQKLKEGWQPYGSALISTAGYGA
                     EFIQPVVSEGSISSPEEPGNRPTTSAPSVAPEYYYVIALAGQSNGMSYGEGLPLPDTF
                     DSPDPRIKQLARRSTVTPGGAVCKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGT
                     VGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGADGTYSDASGASENSTRWGVDK
                     PLYKDLIGRTKAALKKNPKNVLFAVVWMQGEFDFGGTPANHAAQFGALVDKFRADLAD
                     MAGQCVGGSAGGVPWICGDTTYFWKQ"
BASE COUNT          319 a          322 c          390 g          330 t
ORIGIN      
        1 caagtgtaat ctgtgtcaca attcagtcag ttgacagttg cctgtcagac tgagcatttg
       61 ttaaaaaaat ttcgcatggt gaatccccct gtgtggaggg gcgactggtg aaaaatcctt
      121 gcttgtgatt cattatcgac acgggttcgg tggtaccagg ccgaactcac agggaggcac
      181 ccggcaccat gcagtataca gagattaggc atataccaag gcctctcata gcaggggcct
      241 ttttacatgt aaaaaagccc gagtgggttc gggcaattgc atgagatact cgttttaata
      301 atcgaaagca ttttaaccag gattcataag gctgcgcaac tgcgcggcct ttttcgtatt
      361 tcgggctgta gtcttccttc tgccattgtc ctgtaacttc cggacttcag cccgctcctc
      421 atctgactca cacattatcc cgaccgggag gattcatggc atttaaacac tatgatgttg
      481 tcagggcggc gtcgccgtca gaccttgcga aacgaataac tcaaaaactg aaggaagggt
      541 ggcagcctta tggtagtgcg ctgatttcga cagctggtta tggtgcggag ttcatccagc
      601 cagttgtgag tgaggggagc atctcatcac cagaggagcc aggcaaccgt ccgacgacct
      661 cagcgccttc tgttgcgcca gaatattact atgtgatcgc gcttgctggt cagtccaatg
      721 gtatgtcata cggtgaggga ctgccattgc cggatacatt cgacagccct gatccacgta
      781 ttaaacagtt agcgcgtcgc agtacggtga caccgggcgg tgcagtatgc aaatataacg
      841 acatcattcc ggcggaccat tgtctgcatg atgtgcagga catgagccgc cttaaccatc
      901 cgaaagcgga cctgtcaaag gggcagtacg gaaccgtggg gcaggggctg catatcgcca
      961 aaaaattgct gccgtttata ccggcgaatg cgggcattct gctggttccg tgctgtcgtg
     1021 gtggttcagc gttcaccacc ggagctgatg gcacatacag tgacgcgagt ggtgcctcgg
     1081 agaattcaac ccgctggggt gtggacaagc cgctgtataa ggaccttatc ggtcgaacaa
     1141 aagcagcact gaagaagaat ccgaaaaatg tgctgtttgc cgtggtgtgg atgcaggggg
     1201 aatttgattt tggcggtacg ccggcaaatc acgcagcaca gtttggtgcg ctggttgata
     1261 aattccgtgc agacctggcg gatatggcag gtcagtgcgt cggtggctct gctggcggtg
     1321 ttccctggat atgtggagat acgacgtatt tctggaagca g
//
LOCUS       DBFIFI010000417         1356 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.419, whole genome shotgun sequence.
ACCESSION   DBFIFI010000417 DBFIFI010000000
VERSION     DBFIFI010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.419"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            96..974
                     /locus_tag="N7B39_004994"
     CDS             96..974
                     /locus_tag="N7B39_004994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU2424774.1"
                     /translation="MTLTQINSLLAVLDYGGFTEASKRLFMTQSAVSQAIAALEQELG
                     VNILLRDRRKAIQLTTAGHRIVQHLRAINREVNAVKEIAEQEKQNPQRTLRLGCFPSV
                     CACILPVVIRYFEIHHPNIKIIPFEENSTAIIDSLQNESIDAGFVHFPVSGMYSVPIY
                     QDKFTVVLPPNHALAKNSTITVEELMGEPLIISKGRYELSIMALFKEKNITPQIKYEF
                     NHPDTAISFIRQGLGIALLPELTLKTIADELCSVPLEPTFYRQISLLAKEKPVEGSPL
                     FLLQMCTEQLVVSGKI"
BASE COUNT          412 a          275 c          283 g          386 t
ORIGIN      
        1 gtcaaattgt gatgtacttt gcagaacaga agtataaatg gcggcatgct attagagaaa
       61 ttaatcgttt taatattatt gattagcaaa ctctaatgac tcttacccaa ataaattctt
      121 tactcgccgt gctggattac ggtggcttta ctgaagccag caagcgattg ttcatgacgc
      181 agtcggcggt aagtcaggca attgctgccc tcgaacaaga acttggtgtg aatatactgt
      241 tacgcgatcg ccgtaaagct atacaactga ccacagcagg tcaccgcatt gtgcaacatc
      301 tgcgcgccat taatcgggaa gttaacgcag taaaagagat agccgagcag gaaaagcaga
      361 accctcaaag aacgctgaga cttggttgtt tccccagcgt ctgcgcctgt atcttaccgg
      421 ttgtgattcg ctactttgaa attcatcatc cgaatataaa aattattcct tttgaggaaa
      481 atagcacagc gattattgat tcgcttcaga atgaatccat tgatgccggt tttgttcatt
      541 ttccggtgag cggaatgtac tccgtgccga tttatcagga taaattcacc gtagtgttac
      601 cgcccaacca tgctctggca aaaaacagca ctattacagt ggaagaatta atgggtgaac
      661 cattaatcat tagtaaagga cgctatgaat taagcattat ggcactattt aaagaaaaga
      721 atattacccc acagataaaa tatgaattta atcacccgga tacagcgatc agttttattc
      781 gacagggatt aggcatcgcc ttattaccag agttaacgct gaaaaccatt gcagatgaat
      841 tgtgttccgt tcctctcgaa ccaaccttct atcgacagat ttcgttgctg gctaaagaaa
      901 agccggtaga aggcagtcca ctgtttttac tacaaatgtg tacggaacaa ctagtggtga
      961 gtggaaaaat ttgatgtaag acatgagatt gatgacaaat gcaagactgc ctgattcgcc
     1021 acacttagtt gtactatagc ctttaccagg cgtaataatt cgttttcacc tgattttggc
     1081 ccactttgtg aattggcgat atatttatcc agttgggaga agtgacaatg actccaggta
     1141 gaaaccggta tgaagcgccc gactcactgt caaaaccgat tgttgagagt aatgacactc
     1201 cggcgaacag tacacagttt actctggagc cattctcaat cttcaagtgg gatacctaat
     1261 aacttgtcag ggaagttatc aatatctgtt tgttacttat taccgtattt attaacaata
     1321 atgatacttt ctaacatgcg taaaggtctt cgtaat
//
LOCUS       DBFIFI010000418         1355 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.420, whole genome shotgun sequence.
ACCESSION   DBFIFI010000418 DBFIFI010000000
VERSION     DBFIFI010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.420"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            41..445
                     /gene="stpA"
                     /locus_tag="N7B39_004995"
     CDS             41..445
                     /gene="stpA"
                     /locus_tag="N7B39_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HGU2424775.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            complement(498..1016)
                     /gene="ygaP"
                     /locus_tag="N7B39_004996"
     CDS             complement(498..1016)
                     /gene="ygaP"
                     /locus_tag="N7B39_004996"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HGU2424776.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPAKLRRDQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKKAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVILGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQ"
     gene            complement(1026..1325)
                     /locus_tag="N7B39_004997"
     CDS             complement(1026..1325)
                     /locus_tag="N7B39_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HGU2424777.1"
                     /translation="MTELTQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
BASE COUNT          359 a          320 c          325 g          351 t
ORIGIN      
        1 aaatactttt ttgttttggc gttaaaaggt tttctttatt atgtccgtaa tgttacaaag
       61 tttaaataac attcgcaccc tccgtgcgat ggctcgcgaa ttctccattg acgttcttga
      121 agaaatgctc gaaaaattca gggttgtcac taaagaaaga cgtgaagaag aagaacagca
      181 gcagcgtgaa ctggcagagc gccaggaaaa aattagcacc tggctggagc tgatgaaagc
      241 tgacggaatt aacccggaag agttattggg taatagctct gctgctgcac cacgtgctgg
      301 taaaaaacgc cagccgcgtc cggcgaaata taaattcacc gatgttaacg gtgaaactaa
      361 aacctggacc ggtcagggcc gtacaccgaa gccaatcgct caggcgctgg cagaaggtaa
      421 atctctcgac gatttcctga tctaattact gaggccggat gtcgctgcta gggcgcgtcc
      481 ggcgtcgctt ctcaagctca ttggttccac ggcatcttat ctaacaacct tgccatccca
      541 caaaaaccgc taattcctgc aaacagtaac ccggcaccaa caaagccgct taataagaag
      601 aaaccgctat ttacggtata acccagtata acgccgatta atattaaacc acccgcagcg
      661 atctgcacct ggcgcattaa cggcaagggt tgagatttat ttaccgctac tggtaatcct
      721 gcttttttcc agccatcaat cccatcttcg agtaaaaaga tttctgcggg ggcggcaatc
      781 gctgctaatt tatcggcgtt attactggtg cgtttacctg cctggcagtg gaatataatt
      841 tgatcgcgac gtaatttagc cgggagacct gactgttcca gcacggataa tggagccaga
      901 tctgcttcag gaatatgttc acgaagatat tcatcagcgt cacgaatatc gattaacttt
      961 gcgccgcgtg cgattaattc ttgtgcatca tgcggcgaaa tggttgtcaa agccatagtg
     1021 actccttacg gacaatagac atttttcagg gtggcgataa tggcatttac cgcctcattt
     1081 ttaatggaat atagaatgcg ttgggcatcc cgttggctgt tgataagccc ttcgtcccgc
     1141 atacgagcga gatgctgtga tgtcgcagag gcgctcagtc cggtaatgcg cgtcagctct
     1201 cccgcgctgg tgccggggga accgctaagc atgcacagaa tcagcaaccg tttagggtgg
     1261 ctcattgctt tcaataaggc cgctgcctgt tcagcactgg cctgtaattg cgtgagttcg
     1321 gtcatagtta tttaagtatt ttctaaatta agtaa
//
LOCUS       DBFIFI010000419         1351 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.421, whole genome shotgun sequence.
ACCESSION   DBFIFI010000419 DBFIFI010000000
VERSION     DBFIFI010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.421"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(1..1197)
                     /locus_tag="N7B39_004998"
                     /pseudo
     CDS             complement(1..1197)
                     /locus_tag="N7B39_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536548.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
BASE COUNT          391 a          297 c          248 g          415 t
ORIGIN      
        1 cccggatatt tttttcataa taagaaagct gaagagaagt aaatagcgcc agccttctct
       61 ccggaatcat caatggtttg actttcagta tcatgaagtt taacaacaaa tcaattctgt
      121 ttctgaatcc aatccgcctg tcagaaaggt aatgaatata agccatcagg tcggttgttt
      181 tttgtgagga aaatcggtat ttgtgagaaa aaagctcctc cggtcgaaga ttctgactct
      241 gccattgctg caggtattta tactcaatat tcagctcatt aatttctgct tttaacgaag
      301 cgacctggtt aatggcattc aggtgttttt tcacttgttg aagtacatca tcaatcgcat
      361 ttgcagacgg tgagtcaggt aagacttcct cagaacgcga aatcgacgtt gaaaaaaact
      421 gttgcctgtt ctccgtactc cccagtcgtg ccatcacata tcccagttgc tgtttgtcca
      481 tcttttcata aacattactg acagctgagt tattattgga caaaattgcc actgttttat
      541 tctgaattaa tatattagca acaatattga gtatagtctg tgttttacct gttccaggtg
      601 gtccttcaat aactgaaatc tgtgaggaaa atgcccgttc gacagccagc aactgacttt
      661 cattaagacc aaacggaaaa atcaaacccc atggcgaatc acgtttttac tctgtcctga
      721 acagtatgca tgcaaggcgg tatccggatg aggaactatt ttctttatct gcctaaggat
      781 attctcatcg atatttctgt tattaccacg ggcataaaaa atacgttcat tagccaccct
      841 acacaagtaa ttaaacacgg catgctctgt aaatgctgaa cactgttgaa agtcaaggcc
      901 ggtcgttttc atgatgtagg gtcgttcacc ctcgcggtaa taaacagcag catatttatc
      961 accatatata ataaccagtt cagcccggga tgttacagcg ttccctttcc tttcgaggaa
     1021 aacatttcgc aaatttacag ttggcgttat ctgacataca gaaagcggac gagtatattt
     1081 tctcccgctg cgaaaatgac aggtcagcat aacctctctg tcgtcatttc cagcccacag
     1141 cgtccagtcg gtaattttat gagtcttatt ttcaccgtca acacaaatcg tgaccatatt
     1201 cttactcaga aattatcatt taacttacgg aaaattgtta aggcactccc gatattagct
     1261 ccggaggatt ttccacaact ccgggactgt taacatattt ctgcctgcca tatatacatt
     1321 gaatttagtc ataacagtca ataccagaat t
//
LOCUS       DBFIFI010000420         1335 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.422, whole genome shotgun sequence.
ACCESSION   DBFIFI010000420 DBFIFI010000000
VERSION     DBFIFI010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.422"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..1231)
                     /locus_tag="N7B39_004999"
     CDS             complement(<1..1231)
                     /locus_tag="N7B39_004999"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HGU2424778.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKEGEDPGYTL
                     YDLSERLRLRGWQVPAFT"
BASE COUNT          325 a          357 c          349 g          304 t
ORIGIN      
        1 gagtgaaggc cggaacctgc cagccgcgca gacgcagacg ttcagagagg tcgtacaggg
       61 tgtatcccgg atcttcaccc tctttcagtt tgaagcaaac cgccgggatg ccttcgtccg
      121 gacgacccgt acagatgaac tcatacggcc ccagtttggc gatttcatcc gccagataag
      181 cggcaacctg gtaagaggcg ttctgtactt tggtatagcc ttcacgaccg aggcgcagga
      241 attcataata ctgcgcaatc acctgacccg ccgggcggga gaagttgatg gcgaaagtac
      301 caatctgacc accgaggtag tcaacgttga acaccagttc ctgcggcagc gcttcttcat
      361 cacgccagat cacccagccg cagcccagcg gagccagacc gaatttatgg cctgaagcac
      421 tgatcgattt cacacgcggc aggcggaagt cccagacgat atccggggcg acgaacggtg
      481 ccaggaagcc accgctggca gcgtcgatgt gcatgtcgat gtcgataccg gtgtcggcct
      541 ggaatttatc cagtgcatcg tgcagcggtt gcgggaactc atagttaccg gtgtaggtca
      601 cgccgaaagt cggcaccacg ccgatggtgt tttcgtcgca ggcttcaatc atgcgtttcg
      661 ggtccataaa caactgaccg gggcgcatag ggatctcgcg cagctccaca tcccagtagc
      721 gagcgaattt atgccagcag atttgcaccg gaccgcacac caggtttggt ttatccgttg
      781 gtttgcctgc agcttccata cgcttgcgcc aacgccattt catcgccatc ccgccaagca
      841 tacaagcctc ggaagaacca atggtgttgg tgccaacggc ctgaccattt ttcggcgcag
      901 gcgcatgcca cagatcggca accatgttta cgcaacgcag gtcgatggct gcggattgcg
      961 gatattcttc tttgtcgatc cagtttttat tgatcgacaa atccatcaat ttatggacgt
     1021 tttcgtcgtc ccaggtctgg cagaaagtgg ccaggttctg acgagcgttg ccatcaagat
     1081 ataattcatc attgataatc tggaatgcga catcatcgcg catttcgtgc agcggaaatc
     1141 gttttgactc cgcgatagta gaaatggcct ttgcgccaaa acgtgaatcg agtagttctg
     1201 agcggaaatc cgttaacagc ttctggtcca tttcgaactc cttaaattta tttgaaggca
     1261 ataaaaaagt aggatttatc cgcaatggaa gcaaggcatt acaggcttaa tttaaataac
     1321 aaaatcctaa gcaga
//
LOCUS       DBFIFI010000421         1328 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.427, whole genome shotgun sequence.
ACCESSION   DBFIFI010000421 DBFIFI010000000
VERSION     DBFIFI010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.427"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            216..1175
                     /locus_tag="N7B39_005000"
     CDS             216..1175
                     /locus_tag="N7B39_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A deacylase LpxR family protein"
                     /protein_id="HGU2424779.1"
                     /translation="MKKSVIAGVFIALSFTTCSAIANSLALSLANDDAGKFQPILNDI
                     YGNKHENRDDYSQGLFLGYSHDISDSSQLSLHIAQDIYSPSGSNKRHNTAVTGDRAFS
                     AYTHTGIEWNSLANDWIRYRLGTDIGVVGPDAGGQKVQNKAHEIIGAEKYHAWDDQIE
                     NRYGYTVKGMLSMTPSMDILGANVGLYPEVSAVTGNLFQYVAYGATIAIGNDKTFNSD
                     NGFGLLAPRGLMHMSDTSGFKYKIFAGMERRDVNRNYTLEGKTIQTKQTTVSLNKTVD
                     EYQVGATIGYAPVAFTLAFNKVTSEFKTGDDYSFINGAITFFF"
BASE COUNT          494 a          249 c          232 g          353 t
ORIGIN      
        1 tttaataaaa taaaattata aaaaacataa aacaccacgc aaacacactt aataaaaaca
       61 ccgttacatt aaaagataat aaaaaccgca ataaaaaacg aataaatcaa ttgtctcacg
      121 caattataaa acatcatatt gattacgcac cttgcattac aaactcatgt atgtaaaata
      181 cgcgcaccat tcaaaaaaaa ggaagacaat aacatatgaa aaaaagtgtc atcgctggcg
      241 tctttattgc tctgtcattt accacgtgtt cagctatcgc gaacagcctt gcattatcat
      301 tagcaaatga tgatgcaggg aagtttcaac caatacttaa tgatatttat ggcaataaac
      361 atgaaaacag agatgattac tcacaaggct tatttctggg atatagccac gatatctcag
      421 actcgagcca attatctctc catattgcgc aagatattta ctctccatca ggcagtaata
      481 aaagacacaa cacagctgta actggagaca gagcttttag tgcatacact cacactggta
      541 ttgaatggaa ctcccttgcg aatgactgga ttcgctatcg attaggtact gacataggtg
      601 ttgttggccc cgacgcaggc ggtcagaaag tacaaaataa agctcatgag attattgggg
      661 cagaaaaata tcatgcatgg gatgatcaaa tagagaatcg ctacggttat actgtaaaag
      721 ggatgctatc catgacacca agtatggata ttttaggtgc taatgttgga ttataccctg
      781 aagtttctgc tgttactgga aacttatttc aatatgtagc atatggcgca accattgcca
      841 ttggtaatga taaaaccttc aattcggata atggctttgg tctgctggct ccccgtggtt
      901 taatgcatat gtccgataca agcggattca aatacaagat ttttgcaggt atggaaagac
      961 gagatgtcaa tcgcaactat actctcgaag gaaaaacaat acagacgaaa caaacaacag
     1021 tatcgctaaa caaaactgtt gatgaatatc aagttggcgc aacaattggg tatgcacctg
     1081 tagccttcac actagcattt aataaagtaa catcagaatt caagacaggg gatgactatt
     1141 catttataaa tggagcaatc accttctttt tttaactgaa ttgaattcaa tcaaaataac
     1201 ataagtccaa caaaaacata aagtgcgaaa tgaatgccag ctccatttat ttcgcactat
     1261 aaaagattaa aagttgcaat aaaataataa aatgactcag ttacgaaaac caataaactg
     1321 tggccagt
//
LOCUS       DBFIFI010000422         1327 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.428, whole genome shotgun sequence.
ACCESSION   DBFIFI010000422 DBFIFI010000000
VERSION     DBFIFI010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1327
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.428"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            112..714
                     /gene="ais"
                     /locus_tag="N7B39_005001"
     CDS             112..714
                     /gene="ais"
                     /locus_tag="N7B39_005001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HGU2424780.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLGRLAQQHPVVVLFRHAEHCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKDKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(753..1178)
                     /gene="nudI"
                     /locus_tag="N7B39_005002"
     CDS             complement(753..1178)
                     /gene="nudI"
                     /locus_tag="N7B39_005002"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HGU2424781.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEPGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLAHYDLNVATRKTLRLKGLL"
BASE COUNT          359 a          288 c          284 g          396 t
ORIGIN      
        1 ttgccagcat cgtaggattt ttaatattac accaactgct aattatcgcc agaaatgtcg
       61 ctcaatttca cggtaattgt ctggttgcgc ttgtctatag gtggagttta cgtgttagct
      121 ttttgccgct catcattgaa gtcaaaaaaa tatttcatca ttttactggc gctcgctgca
      181 attgccggac tgggtactca tgccgcctgg agtagcaatg gtttgccacg tatcgacaat
      241 aaaacgctgg gcagactggc acagcagcac ccggttgtcg ttttgtttcg ccatgctgaa
      301 cattgcgatc gttcaaccaa tcaatgcttg tcagataaaa caggtattac ggttaaaggt
      361 acccaggatg cccgtgaact gggcaacgct tttagtgctg atatccctga tttcgatctt
      421 tattccagta ataccgtccg gaccattcag tccgcgacct ggttttcagc gggtaaaaaa
      481 ttgacggtag ataaacgact tcttcagtgc ggtaatgaga tttatagtgc gattaaggac
      541 ttacaaagca aagcgcctga taaaaatatc gttattttca cccataatca ttgcctgaca
      601 tatattgcta aagataagcg tgatgcgaca tttaaacctg attatctgga tggtttagtc
      661 atgcatgtgg aaaaaggcaa agtttatctg gatggggaat tcgttaatca ctaaattttc
      721 aatctgacag ccagtaatgg ctgtcatcat tgttacagaa gacctttcaa acgtaacgtt
      781 tttcgggtgg cgacattcaa atcatagtgc gccagatctt caggttttac ccacgcgtag
      841 tcctgaaact cttcgtttat tttcacttct cggttggcag aaacgcaatc aaaaatcagg
      901 taaatcatat aaatttcttc cttgcgacca tctgcatacg tcttggtgcg aatatcatcg
      961 ctgaaggtcc agggcgtgat ttctgtcaaa agcagctgtt ctcccagttc ttcgcgaatt
     1021 tcgcggcgta gagcctcttc aattcgttcg ccgggctcca cgccgccacc cgaaagcgcc
     1081 cattgaccgg ggaaaacgcc gcgatcgtcg gccattttac acagcaaata agcaccatca
     1141 ttttgaatca aagggcatac aatagtccgt tgtcgcacag gggtattcct tataaataaa
     1201 aagaaaccat gagaatacgg taactcagaa atatgtgaaa gtgacagtaa tctcttttgc
     1261 gatgataact gcacttatta ttatttttgt gtgtatcggt aaactgtgtt tattttttcg
     1321 gcgttta
//
LOCUS       DBFIFI010000423         1321 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.429, whole genome shotgun sequence.
ACCESSION   DBFIFI010000423 DBFIFI010000000
VERSION     DBFIFI010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.429"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            73..177
                     /locus_tag="N7B39_005003"
                     /pseudo
     CDS             73..177
                     /locus_tag="N7B39_005003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            complement(297..1316)
                     /gene="nleC"
                     /locus_tag="N7B39_005004"
     CDS             complement(297..1316)
                     /gene="nleC"
                     /locus_tag="N7B39_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector zinc
                     metalloprotease NleC"
                     /protein_id="HGU2424782.1"
                     /translation="MICRVLDINMKIPSLQSNFNFSAPAGYSAPIAPNRAENAYADYV
                     LDIGKRIPLSAADLSNVYESVIRAVHDSRSRLIDQHTVDMIGNTVLDALSRSQTFRDA
                     VSYGIHNEKVHIGCIKYRNEYELNEESSVKIDDIQSLTCNELYEYDVGQEPIFPICEA
                     GENDNEEPYVSFSVAPDTDSYEMPSWQEGLIHEIIHHVTGSSDPSGDSNIELGPTEIL
                     ARRVAQELGWSVPDFKGYAEPEREAHLRLRNLNALRQAAMRHEENERAFFERLGTISD
                     RYEASPDFTEYSAVSNIGYGFIQQHDFPGLAINDNLQDANQIQLYHGAPYIFTFGDVD
                     KHNQQ"
BASE COUNT          351 a          293 c          273 g          404 t
ORIGIN      
        1 agtgaatcac ggtacttttc tgtgcacaac gtactgcagt ttctttacga tttatagctg
       61 tactgttgaa ttatgtacaa tttgaatcca tgcatgacgt gtgatgccta ttgtgcattt
      121 ttccgagcct ctttttactg ggccgaagcc gatgatgctg atagtctcgt tccggccatg
      181 ttcacttact acagtaaaat taggggaatt gcctttacat acaatgttgc acgattaccc
      241 atcatcaact ccgttaaaat gtatgaatag taaccttatg tcactgcaaa gacgaatcat
      301 tgctgattgt gtttgtccac atccccaaat gtaaaaatat aaggggcgcc atgatacagt
      361 tggatctgat ttgcatcctg taaattatcg ttgatagcca atccaggaaa atcatgttgc
      421 tggataaatc cgtatcctat gttagacaca gcggaatact ctgtgaaatc aggactcgcc
      481 tcatatcggt cactgatcgt acccagcctt tcgaagaaag ccctctcatt ctcttcatgc
      541 ctcatggcag cctgtcgaag ggcattcagg ttacgtagcc taagatgagc ttcacgttct
      601 ggctctgcat atcctttgaa gtcgggaaca ctccatccca gttcttgagc gacacgacgt
      661 gcgagaatct cggtgggtcc taactctata ttactatctc cagatggatc gctagatcca
      721 gtaacatgat gaataatctc gtgaatcagt ccttcctgcc acgatggcat ctcataagag
      781 tcagtatctg gcgcaacact aaaactgaca taaggctctt cgttatcgtt ttctcctgct
      841 tcgcaaatgg ggaaaattgg ctcttgcccg acatcatatt catataattc gttacaggtt
      901 agtgattgaa tatcatcaat tttgacagaa gattcttcgt taagctcgta ttcgtttctg
      961 tatttaatgc aaccaatgtg taccttctca ttatgaatgc catagcttac ggcatcacga
     1021 aatgtctgtg atcggctcaa agcatcaagt acagtgttgc cgatcatatc gactgtatgc
     1081 tgatcgataa gcctgctacg gctgtcatgg acggcgcgta ttacactttc gtatacgttg
     1141 cttaaatctg ctgcggaaag tggtattcgc ttacctatat ccaaaacgta atccgcatag
     1201 gcattttcag cacgattagg agcaatggga gcagagtatc ctgccggggc ggaaaagttg
     1261 aagttggact gtaatgaggg aattttcatg tttatatcta ataccctgca aatcatttat
     1321 t
//
LOCUS       DBFIFI010000424         1319 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.430, whole genome shotgun sequence.
ACCESSION   DBFIFI010000424 DBFIFI010000000
VERSION     DBFIFI010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.430"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            125..328
                     /locus_tag="N7B39_005005"
     CDS             125..328
                     /locus_tag="N7B39_005005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HGU2424783.1"
                     /translation="MSSIFLTENELQTLTGCKYASHQRNWLIKNGLPFYTNRSGKPIV
                     SRELFTCIKTLPPREAEPDFGAI"
     gene            328..>1319
                     /locus_tag="N7B39_005006"
     CDS             328..>1319
                     /locus_tag="N7B39_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU2424784.1"
                     /translation="MGRRRKNPEHEKLPPKVYPNKYSVWKPTSRESVTLTAIEDGLAA
                     LWKKYEETVNHRDRAMTFGRLWEKFLASAYYSELSPRTQKDYLQHQKKLLAVFGKVLA
                     DSVKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAKN
                     RERYVTDKEYQAVLSVAPLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGKT
                     GKKQIKAWSPRLQAAIEKAKQLPTSAYVISNQYGNRYMYKGFNEMWVEARNHAGKISG
                     ILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLP"
BASE COUNT          441 a          274 c          310 g          294 t
ORIGIN      
        1 cgttaccagg caaaatacgt ttcagccatc tggcaaaaac tgctcccgag tcaggctgat
       61 ttttaatgtt caacccggat cgccgctaac ggcatatagt tagcggcggt tgtgagatat
      121 agctatgagc agcatatttt taaccgaaaa tgaattgcaa acgttaacgg gctgcaaata
      181 tgcaagccac cagcgcaact ggttaataaa aaatgggctt ccgttctata ccaatcgtag
      241 tggaaaaccg atcgtcagtc gggaactgtt tacctgtata aaaactttac caccacgtga
      301 ggctgaacct gatttcggtg cgatctaatg ggaagacgaa ggaaaaatcc cgaacacgaa
      361 aaattaccgc caaaggtata ccccaataaa tatagtgtat ggaaaccgac atccagagaa
      421 tctgtaacct taactgcaat cgaggatggt ttagccgcat tatggaaaaa gtatgaagaa
      481 acggttaacc atcgcgatcg cgcaatgaca tttgggcgtt tgtgggaaaa atttctcgcc
      541 agcgcctatt acagcgagct tagtcctaga actcaaaaag attatctgca acatcaaaaa
      601 aagctgctgg ccgtattcgg taaggtgctg gccgattctg taaaaccaga gcacatcaga
      661 cgatacatgg acaaaagagg cgagcagagt aaaacgcagg caaaccatga aaaaagcagt
      721 atgtcgcgcg tttatagttg ggggtatgag cgaggatacg tgaaggctaa cccatgtgca
      781 ggtgtaagta aattcaaggc caaaaaccgc gaacgatatg taaccgacaa agaataccag
      841 gcagtattaa gcgttgcacc tcttcctgtt tttatcgcaa tggaaattgc ctatctgtgt
      901 gcagcgaggg tttccgatgt gttatcgctg aaatgggagc agattggaaa cgacgggatc
      961 tttatccagc aagggaaaac agggaaaaaa cagataaaag catggagtcc acgattacag
     1021 gcggcgatcg aaaaagcaaa acagttacca acatccgcct atgtaatcag caatcaatac
     1081 ggcaaccgat atatgtacaa aggctttaac gaaatgtggg tagaagcaag aaatcacgca
     1141 ggcaaaattt caggtatttt aaccgacttc acctttcatg atctgaaggc gaaaggaatt
     1201 tcagactatg aaggaagcag ccgggataag caacttttct ctggtcacaa aaccgaaggg
     1261 caagtgctaa tctatgacag gaaggttaaa gtttcaccaa cacttgatgt cccgttacc
//
LOCUS       DBFIFI010000425         1308 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.431, whole genome shotgun sequence.
ACCESSION   DBFIFI010000425 DBFIFI010000000
VERSION     DBFIFI010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.431"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..428
                     /locus_tag="N7B39_005007"
     CDS             <1..428
                     /locus_tag="N7B39_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HGU2424785.1"
                     /translation="LLDASNPRLTVSKQRNAITQQLIDNLIKGLLQTMPYASNISIAV
                     LDDNVLESQAIETLSAIGLSYEKYKTANNVYFNIIGTLSDSELNKINNYVDEYYKQWG
                     KQYVRFNVNLKNQDTNNSSFSYGDNRFEKSQGSKWTFQE"
     gene            442..681
                     /locus_tag="N7B39_005008"
     CDS             442..681
                     /locus_tag="N7B39_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex
                     protein"
                     /protein_id="HGU2424786.1"
                     /translation="MADWNGYIMDISKQFDQGVDDLNQQVEKALEDLATNPSDPKFLA
                     EYQSALAEYTLYRNAQSNVVKAYKDLDSAIIQNFR"
     gene            701..1033
                     /gene="sctI"
                     /locus_tag="N7B39_005009"
     CDS             701..1033
                     /gene="sctI"
                     /locus_tag="N7B39_005009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
                     /protein_id="HGU2424787.1"
                     /translation="MSVSNMPPIDRAEQSTAHEIQQAKVIDLNDRVLNLDNPDDKMIS
                     AFANYAVQTENWQQNALQALTSDKKGLTPEKLLVLQDHVLNYNVEVSLVGTLARKIVA
                     AVETLTRS"
     gene            1030..>1308
                     /locus_tag="N7B39_005010"
     CDS             1030..>1308
                     /locus_tag="N7B39_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="required for invasion of epithelial cells; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
                     /protein_id="HGU2424788.1"
                     /translation="MKYISLLLFILLLCGCKQQELLNHLDQQQANDVLAVLQRHNINA
                     EKKDQGKTGFSIYVEPTDFASAVDWLKIYNLPGKPDIQISQMFPADALV"
BASE COUNT          453 a          224 c          261 g          370 t
ORIGIN      
        1 agctattaga tgccagtaat cccagactga ccgtgagtaa gcaacggaat gctatcacac
       61 agcaactaat cgacaatctt attaaagggt tactacaaac tatgccatat gccagcaata
      121 taagtattgc ggtattagat gataatgtat tggaaagtca ggctattgaa acattgtctg
      181 caataggtct ttcttatgaa aaatataaaa cagctaacaa tgtgtatttc aatatcattg
      241 gtacgttaag cgacagtgaa ttaaacaaaa taaataacta tgttgacgaa tattataaac
      301 aatggggtaa acaatatgta agatttaatg tgaatttgaa aaatcaggac acaaataata
      361 gttcatttag ctacggagat aaccgattcg agaagtctca aggtagcaag tggacatttc
      421 aggaataatt gaggaggact tatggcagat tggaatggtt atattatgga catcagcaaa
      481 caatttgatc agggcgttga tgatctgaac cagcaagttg aaaaagcgtt ggaggattta
      541 gcaaccaatc cttccgaccc gaaattcctt gccgaatatc aaagtgcatt agctgagtat
      601 acattatatc gaaatgcgca atctaacgtt gtaaaagcgt ataaagatct tgattcggca
      661 atcatacaaa acttccgcta aactggataa ggattttatt atgtcagtaa gcaatatgcc
      721 acctattgat agagctgagc aatcaactgc tcatgaaata cagcaagcga aagtgattga
      781 tttaaatgac cgggttctca atcttgacaa cccggatgat aaaatgatct ccgcttttgc
      841 taactatgct gtacaaactg aaaattggca gcaaaatgcg ttacaagcat taacgtctga
      901 taaaaaagga ttaactcctg aaaaattatt agtcttgcag gatcatgtgc ttaattataa
      961 tgtcgaggtt tcattggtgg gaacattagc aagaaaaatt gtcgctgcag ttgagacgtt
     1021 gacccgctca tgaaatatat atcattatta ttgtttatcc tgctgttgtg cggctgcaag
     1081 cagcaggaat tactgaacca tcttgatcaa cagcaagcta atgatgtcct tgctgtttta
     1141 caaagacata atattaatgc tgaaaagaag gatcaaggca aaactggatt ctcaatctat
     1201 gttgaaccaa cggattttgc ttctgcggtg gattggctaa aaatctacaa tctcccgggt
     1261 aagcctgata ttcaaatatc gcagatgttc cctgcagatg cactcgtt
//
LOCUS       DBFIFI010000426         1308 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.432, whole genome shotgun sequence.
ACCESSION   DBFIFI010000426 DBFIFI010000000
VERSION     DBFIFI010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.432"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..376)
                     /locus_tag="N7B39_005011"
     CDS             complement(<1..376)
                     /locus_tag="N7B39_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HGU2424789.1"
                     /translation="MTFKHYDVVRAASPSDLAERLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVAVTGNEGKAVAVSATRAPEYYFVVVLAGQSNGMSYGEGLPLPETY
                     DRPEPRIKQLARRSTVTPGGAAC"
     gene            620..943
                     /locus_tag="N7B39_005012"
     CDS             620..943
                     /locus_tag="N7B39_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HGU2424790.1"
                     /translation="MNWIVIDTVIQPACGISFSAIWCNIKLILWYQSDTFLPPGCIFT
                     PTRTGVILNNKELPVTIYNVAPFNKNVWNLIKNSQECPANTGNITDKCFNHRCILEIC
                     PYGRK"
     gene            complement(1240..>1308)
                     /locus_tag="N7B39_005013"
     CDS             complement(1240..>1308)
                     /locus_tag="N7B39_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin subunit B"
                     /protein_id="HGU2424791.1"
                     /translation="VTIKSSTCESGSGFAEVQFNND"
BASE COUNT          383 a          350 c          262 g          313 t
ORIGIN      
        1 tgcatgctgc accacccggt gtcaccgtac tgcgacgcgc cagttgctta atacgcggct
       61 ccggacggtc atatgtctcc ggcagcggaa gaccttcacc atacgacatg ccgtttgact
      121 gccctgccag aaccacaaca aagtaatact ccggggctct ggtggcactg acagccaccg
      181 ccttaccctc attaccggtc accgccactg gtgtggtgac atcaccttcc gccgcaatgg
      241 cctgcatcag ggtgtaaggc gtgatggcca ccggactgcc aaatggctgc cagccctcct
      301 tcagtttttg tgtcagtcgt tcagcaaggt ctgacggcga tgccgccctg accacatcgt
      361 agtgtttaaa tgtcataacc tctcctgccg ccagcaaagt aattatccgg aacacacaat
      421 catgtaacac ataaaaaaca cgctataaaa aaaccatgtc tcttatataa caaaaaaacg
      481 ccgacaattt aaaatgccct cttattttaa acaaagaata aacgacacca gaaaaacaac
      541 tcagcctgac agattgcttt caaaacatca cctataattt atggcatcat ttaaaacctt
      601 aagaaaataa ggaaagcaaa tgaactggat agtgattgac acggtaatcc agccagcatg
      661 tggcatatca ttttcagcca tatggtgtaa cataaaatta atattatggt atcagtcaga
      721 taccttcctc cctccgggct gtatttttac accaacccgg acaggcgtaa tactcaataa
      781 taaagagctc cccgtcacca tttacaacgt ggccccattt aataaaaacg tctggaattt
      841 aattaaaaac agccaggagt gccctgcaaa cacaggaaat atcacagata agtgttttaa
      901 tcatcgctgc attctggaaa tatgcccata cgggcgaaaa taacagaatc attcagataa
      961 aaagcccctc cggagagggg ctgacactgc gtatctgtat catcatgtac atggtgccgg
     1021 gtgcctcccg gtgagttcag tccggtgtcc ctgaacccgc gtatctcgct ccaggttgtc
     1081 gtcagagatg acaccttata caccagtcgc ccctccgcac agggggattc accatgcgaa
     1141 tttttttaac gaatgctcag tctgacaggc aactgtcaac tgactgaatt gtgacacaga
     1201 ttacacttgt tacccacata ccacgaatca ggttatgcct cagtcattat taaactgcac
     1261 ttcagcaaat ccggagcctg attcacaggt actggatttg attgtgac
//
LOCUS       DBFIFI010000427         1304 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.433, whole genome shotgun sequence.
ACCESSION   DBFIFI010000427 DBFIFI010000000
VERSION     DBFIFI010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.433"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            135..482
                     /gene="tnpB"
                     /locus_tag="N7B39_005014"
     CDS             135..482
                     /gene="tnpB"
                     /locus_tag="N7B39_005014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGU2424792.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            532..>1304
                     /locus_tag="N7B39_005015"
     CDS             532..>1304
                     /locus_tag="N7B39_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc8 family transposase"
                     /protein_id="HGU2424793.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DAIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYRQSEIYGRQGVELRRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPG"
BASE COUNT          282 a          379 c          387 g          256 t
ORIGIN      
        1 cagccagaat cgctcgcaga ggacccggaa accctcagta tcagctgtga ggtaacgttc
       61 cggcacggga cgctccgctt caatggcaat gtcagcgaaa agctcctgac tctgctgata
      121 caggaactga agcgatgatc ccgttacctt ccgggaccaa aatttggctg gttgccggta
      181 tcaccgatat gagaaatggc ttcaacggcc tggctgcgaa agtacaaacg gcgctgaaag
      241 acgatcccat gtccggccat gttttcattt tccggggccg cagcggcagt caggttaaac
      301 tgctgtggtc caccggtgac ggactgtgcc tcctgaccaa acggctggag cgtgggcgct
      361 tcgcctggcc gtcagcccgt gatggcaaag tgttccttac gcaggcgcag ctggcgatgc
      421 tgctggaagg tatcgactgg cgacagccta agcggctgct gacctccctg accatgctgt
      481 aaatctcttt atcctggttg tcacagaata agcccggtaa aatacgggct tatgaacgac
      541 atctcttctg acgacatctt cctgctgaaa cagcgcctgg ccgaacagga agcgctgatc
      601 cacgccctgc aggaaaagct gagcaaccgg gagcgcgaaa tagaccatct gcaggcgcag
      661 ctggataaac tccgccggat gaacttcggc agtcgttccg aaaaagtctc ccgccgtatc
      721 gcacaaatgg aagccgatct gaaccggctt cagaaagaga gcgatacgct gactggtagg
      781 gtgtatgacc cggcagtaca gcgtccgttg cgtcagaccc gcacccgtaa gccgttccct
      841 gaatcactac cccgtgacga aaagcgactg ttgcctgcgg cgccgtgctg cccgaactgc
      901 ggcggttcac tgagctatct gggcgaggat accgccgaac agctggagtt gatgcgtagc
      961 gccttccggg ttatccggac ggtacgggaa aaacatgcct gtactcagtg cgatgccatc
     1021 gtgcaggcac ctgcaccttc gcggcccatc gagcggggta tcgccggacc ggggctgctg
     1081 gcccgcgtgc tgacctcgaa gtatgcagag cacaccccgc tgtatcgcca gtcagaaata
     1141 tacggccggc aaggtgtgga gctgaggcgt tcactgctgt cgggctgggt ggatgcatgc
     1201 tgccggctgc tgtctccgct ggaagaggcg cttcatggct atgtcatgac tgacggcaaa
     1261 ctccatgccg atgatacccc ggtccaggta ctgctgccgg gtaa
//
LOCUS       DBFIFI010000428         1301 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.434, whole genome shotgun sequence.
ACCESSION   DBFIFI010000428 DBFIFI010000000
VERSION     DBFIFI010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.434"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            191..1144
                     /gene="ompT"
                     /locus_tag="N7B39_005016"
     CDS             191..1144
                     /gene="ompT"
                     /locus_tag="N7B39_005016"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HGU2424794.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETLSFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
                     KYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVSVNAGYYVTPNAKVYVEGTWNRV
                     TNKKGNTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
BASE COUNT          446 a          269 c          251 g          335 t
ORIGIN      
        1 aaaatttcca gcatattaaa ccacaacaat aatacaacac ttggcaagct gcccataaag
       61 aataaataaa acgaaacaat aattcaaact taaacaaacc ataaacagtt aaacaatata
      121 caattgactc attaagttag atataaaaaa tacatattca atcattaaaa cgattgaatg
      181 gagacctttt atgcgggcca aacttctggg aatagtcctg acaaccccta ttgcgatcag
      241 ctcttttgct tctaccgaga ctttatcgtt tactcctgac aacataaatg cggacattag
      301 tcttggaact ctgagcggaa aaacaaaaga gcgtgtttat ctagccgaag aaggaggccg
      361 aaaagtcagt caactcgact ggaaattcaa taacgctgca attattaaag gtgcaattaa
      421 ttgggatttg atgccccaga tatctatcgg ggctgctggc tggacaactc tcggcagccg
      481 aggtggcaat atggtcgatc aggactggat ggattccagt aaccccggaa cctggacgga
      541 tgaaagtaga caccctgata cacaactcaa ttatgccaac gaatttgatc tgaatatcaa
      601 aggctggctc ctcaacgaac ccaattaccg cctgggactc atggccggat atcaggaaag
      661 ccgttatagc tttacagcca gaggtggttc ctatatctac agttctgagg agggattcag
      721 agatgatatc ggctccttcc cgaatggaga aagagcaatc ggctacaaac aacgttttaa
      781 aatgccctac attggcttga ctggaagtta tcgttatgaa gattttgagc taggtggcac
      841 atttaaatac agcggctggg tggaagcatc tgataacgat gagcactatg acccaggaaa
      901 aagaatcact tatcgcagta aagtcaaaga ccaaaattac tattctgttt cagtcaatgc
      961 aggttattac gtaacaccta acgcaaaagt ttatgttgaa ggcacatgga atcgggttac
     1021 gaataaaaaa ggtaatactt cactttatga tcacaatgat aacacttcag actacagcaa
     1081 aaatggtgca ggcatagaaa actataactt catcactact gctggtctta agtacacctt
     1141 ttaacgaagt taaccagatt ttctccccgg tggtattgta taaccccggg gaatttgttt
     1201 acagttacaa gtagacaata tccactaatt ttgtaagcat ttaacctatg tcataacgtg
     1261 taatctctta ccgcaacaca agacctcaat atattcaaat t
//
LOCUS       DBFIFI010000429         1298 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.435, whole genome shotgun sequence.
ACCESSION   DBFIFI010000429 DBFIFI010000000
VERSION     DBFIFI010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.435"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            27..1172
                     /gene="espG"
                     /locus_tag="N7B39_005017"
     CDS             27..1172
                     /gene="espG"
                     /locus_tag="N7B39_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector EspG"
                     /protein_id="HGU2424795.1"
                     /translation="MINGLNNDSASLVLDAAMKVNSGFKKSWDEMSCAEKLFKVLSFG
                     LWNPTYSRSERQSFQELLTVLEPVYPLPNELGRVSARFSDGSSLRISVTNSELVEAEI
                     RTANNEKITVLLESNEQNRLLQSLPIDRHMPYIQVHRALSEMDLTDTTSMRNLLGFTS
                     KLSTTLIPHNAQTDPLSGPTPFSSIFMDTCRGLGNAKLSLNGVDIPANAQKLLRDALG
                     LKDTHSSPTRNVIDHGISRHDAEQIARESSGSDKQKAEVVEFLCHPEAATAICSAFYQ
                     SFNVPALTLTHERISKASEYNAERSLDTPNACINISISQSSDGNIYVTSHTGVLIMAP
                     EDRPNEMGMLTNRTSYEVPQGVKCIIDEMVSALQPRYAASETYLQNT"
BASE COUNT          434 a          270 c          239 g          355 t
ORIGIN      
        1 ttacaaacca gataggagaa tctctcatga taaatggact taataatgac tccgcatctt
       61 tagttttaga tgctgcaatg aaagttaatt ctgggtttaa aaaaagctgg gatgagatgt
      121 catgcgctga aaagttattt aaagtactta gttttggttt atggaatcca acgtacagtc
      181 gtagtgaaag acaatcattt caagagttgt taaccgtttt agagcctgta tatccacttc
      241 ccaatgaatt aggcagagta tctgctcgtt tttcagatgg ttcatcctta agaatttccg
      301 tcactaacag cgaacttgtt gaagccgaga ttcgcacagc aaataatgaa aagattactg
      361 tgctcctgga gtcaaacgaa caaaataggt tattacaatc tttacccatc gatcgccaca
      421 tgccatacat tcaggttcat cgtgccttat ctgagatgga cctgactgat actacctcaa
      481 tgcgcaatct acttggtttt acgtcaaaac tatcaacaac cttgattcct cataatgctc
      541 aaacagatcc gctttccggg cctacaccat tcagctctat ctttatggat acatgtcgag
      601 gactcggcaa tgcaaagctt tcactcaatg gtgttgatat acctgcaaat gcacaaaaat
      661 tgcttcgcga tgcactagga cttaaagaca cacattcatc accaacccgg aatgttatag
      721 atcatggtat ttctcgccat gatgcagagc aaatagcaag agaaagcagc ggcagtgata
      781 aacagaaagc tgaagttgtg gaatttttat gccatccaga agcagcaacg gccatatgct
      841 cggctttcta tcaatctttc aatgtgccag ccttaacgtt gacacatgaa aggatctcta
      901 aagccagtga atacaatgcg gaaagatcat tagatacacc taacgcttgc attaacatca
      961 gtatctctca atcatcagat ggaaacattt atgttaccag ccatactggg gttctgataa
     1021 tggcgccaga agaccgcccc aacgagatgg gcatgttgac gaacaggact tcttatgaag
     1081 tgccgcaagg tgtgaaatgt ataatcgatg aaatggtaag tgcgctacaa ccaaggtatg
     1141 ccgcatctga aacgtactta caaaacactt aaaataaaac tttattaatc aaaactaata
     1201 atagaaacca cacccataat tattaacaat ttattccgaa gttaatcaca attaaacaaa
     1261 gatatttatt ctgcaaacaa tttacagcaa atagagaa
//
LOCUS       DBFIFI010000430         1290 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.436, whole genome shotgun sequence.
ACCESSION   DBFIFI010000430 DBFIFI010000000
VERSION     DBFIFI010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1290
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.436"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..1195
                     /locus_tag="N7B39_005018"
     CDS             <1..1195
                     /locus_tag="N7B39_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HGU2424796.1"
                     /translation="ARYLGSALYWIAASINIKPGHDYYFYIRSVNTVGKSAFVEAVGR
                     ASDDAEGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITNVSNEITQTVNK
                     KLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIENTPAGMQSQ
                     VLLAADRIAMINPANGNTKPMFVGQGDQIFMNEVFLKYLTAPTITSGGNPPAFSLTPD
                     GRLTAKNADISGNVNANSGTLNNVTINENCRVLGKLSANQIEGDLVKTVGKAFPRDSR
                     APERWPSGTITVRVYDDQPFDRQIVIPAVAFSGAKHEKEHTDIYSSCRLIVRKNGAEI
                     YNRTALDNTLIYSGVIDMPAGHGHMTLEFSVSAWLVNNWYPTASISDLLVVVMKKATA
                     GITIS"
BASE COUNT          346 a          283 c          355 g          306 t
ORIGIN      
        1 agcccgctat cttggctcgg cgctgtactg gatagctgcc agtatcaata tcaaaccggg
       61 ccatgattat tatttttata tccgcagtgt gaatactgtt ggcaaatcgg cattcgtgga
      121 ggctgtcggt cgggcgagcg atgatgcgga aggttacctg gattttttca aaggagaaat
      181 cgggaaaaca catctggccc aggagctgtg gacgcagatt gataacggtc agcttgcgcc
      241 ggacctggct gaaatcagga cgtccattac gaatgtcagc aatgaaatca cgcagaccgt
      301 caataaaaaa ctggaaaatc agagtgcggc aatccagcag atacagaaag ttcaggttga
      361 tacaaataat aacctgaaca gcatgtgggc cgtgaaactg cagcagatgc aggacggacg
      421 cctttatatt gcgggtatcg gtgccggtat tgagaatacg ccagcaggaa tgcagagtca
      481 ggtgctgctg gcggcagaca ggattgcgat gattaatcct gcgaatggca acacaaagcc
      541 gatgtttgtt ggtcagggcg atcagatatt tatgaatgaa gtgttcctga aatatctgac
      601 ggctcccacc attaccagcg gcggtaatcc tccggcattt tccctgacac cggacgggcg
      661 gctgacggcg aaaaatgccg atatcagcgg taacgtgaat gcgaactccg ggacgctcaa
      721 caacgtcacg attaacgaga actgtcgggt tctgggaaaa ttgtccgcga accagattga
      781 aggcgatctc gttaaaacag tgggcaaagc tttcccccgg gactcccgtg caccggagcg
      841 gtggccatca ggaaccatta ccgtcagggt ttatgacgat cagccgtttg accggcagat
      901 tgttattccg gcggtggcat tcagcggcgc taaacatgag aaagagcata ctgatattta
      961 ctcctcatgc cgtctgatag tgcggaaaaa cggtgctgaa atttataacc gtaccgcgct
     1021 ggataatacg ctgatttaca gtggtgttat tgatatgcct gccggtcacg gtcacatgac
     1081 actggagttt tcggtgtcag catggctggt aaataactgg tatcccacag caagtatcag
     1141 cgatttgctg gttgtggtga tgaagaaagc cactgcaggc atcacgatta gctgaatttt
     1201 ataacccaga tacgggcgcc agaaatggcg ccttttttat tgcagaaaag cgagaggtaa
     1261 ttatgcgtaa attatgtgct gttattttgt
//
LOCUS       DBFIFI010000431         1277 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.437, whole genome shotgun sequence.
ACCESSION   DBFIFI010000431 DBFIFI010000000
VERSION     DBFIFI010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.437"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            6..81
                     /locus_tag="N7B39_005019"
     tRNA            6..81
                     /locus_tag="N7B39_005019"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="N7B39_005020"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="N7B39_005020"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(467..1225)
                     /gene="yhdZ"
                     /locus_tag="N7B39_005021"
     CDS             complement(467..1225)
                     /gene="yhdZ"
                     /locus_tag="N7B39_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HGU2424797.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
BASE COUNT          324 a          334 c          304 g          315 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt ggggactccc catgcgagag tagggaactg ccagacatca
      241 aataaaacaa aaggctcagt cggaagactg ggccttttgt tttatctgtt gtttgtcggt
      301 gaacactctc ccaaccagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg
      361 gcccggaggg tggcgggcag gacgcccgcc ataaactgcc agacatcaaa tcaagcgaaa
      421 ggccatccga aaggatggcc tttatgcttt tcgaactaac attcaattaa tggattacct
      481 gcgataaaaa tgccctcgta cgctctgact taggatgcgc aaaaaactca tccggtgcag
      541 cttgctccac tatttcgcca cgatccataa aaatcacccg gtcagcgacg gttcgcgcaa
      601 accccatctc atgggttaca cacaacattg tcatacccga ttgcgccagc ccaatcatcg
      661 tatccagcac ctctttcacc atctccggat cgagcgccga tgtgggctca tcaaacaaca
      721 taattttcgg cttcatacac agcgagcgcg caatggcaac gcgttgctgc tgaccacctg
      781 aaatctgtcc gggaaactta tgcgcatgtt cggcaattct cacccgctca aggtaatgca
      841 tcgccagagc ttcagcctct ttctttggca tcttgcgtac ccaaatcggt gccagggtac
      901 agttctgtag aacggtcaga tgagggaaga gattgaaatg ctgaaagacc attcccactt
      961 cctgcctgac gcgctcaata ttgcggatat cttcattaag ttcgatgcca tctaccacga
     1021 tccgtccctg ttgatgttct tccagatgat taatacaacg aatggttgtc gatttaccgg
     1081 aacctgaagg gccacacaga acgatccgtt ctcccggttg cacggttaaa tttatatttt
     1141 tcaaaacatg gaattgtcca taccatttat tgacgttttc cagcgtaatc atcgcgttag
     1201 caggttgcag taaaatttgg ctcatagtgt cctcaatgcg gtgtacgccc ggtgttaaaa
     1261 cgtttttcca gatgctg
//
LOCUS       DBFIFI010000432         1273 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.438, whole genome shotgun sequence.
ACCESSION   DBFIFI010000432 DBFIFI010000000
VERSION     DBFIFI010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.438"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..78)
                     /locus_tag="N7B39_005022"
     CDS             complement(<1..78)
                     /locus_tag="N7B39_005022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424798.1"
                     /translation="MWIVLVLSLSTLSWHKVVAFSLLTVS"
     gene            complement(230..601)
                     /locus_tag="N7B39_005023"
     CDS             complement(230..601)
                     /locus_tag="N7B39_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HGU2424799.1"
                     /translation="MARDIQMVLERWGAWAANNHEDVTWPSIAAGFKGLIPTKVKSRP
                     KCSDDDAMIICGCMARLNKNNQYLHDLLVDYYVGGMTFMALARKHRCSDGLIGKRLYK
                     AEGIIEGMLMALNVRLDMDMR"
     gene            complement(591..962)
                     /locus_tag="N7B39_005024"
     CDS             complement(591..962)
                     /locus_tag="N7B39_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HGU2424800.1"
                     /translation="MRIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAEVLIDDEQFDEINIVRGQ
                     PVPGGRLGVKIYEIRGGNDGA"
     gene            complement(975..>1273)
                     /locus_tag="N7B39_005025"
     CDS             complement(975..>1273)
                     /locus_tag="N7B39_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGU2424801.1"
                     /translation="SFMKRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQG
                     GMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLIFRFLDHAFATGVLG"
BASE COUNT          367 a          348 c          229 g          329 t
ORIGIN      
        1 agacaccgtc aacaatgaaa aagccactac cttatgccaa ctgagagttg acagtgacag
       61 tactaacaca atccacataa gcactccttt tatttaatga tgaagattga ttatcaatat
      121 tttcaattca ccaggcaaca ttttatctac cttccacaat acatgaccat caaaaaataa
      181 acatgttaat tataaacaca gaaaacataa ccctcatcac tatatatccc taccgcatat
      241 ccatatctaa ccggacattc agagccataa gcattccttc aataatacct tccgctttat
      301 aaagcctttt accaataagc ccatcagaac atctatgctt acgtgcaaga gccataaatg
      361 tcattccacc tacgtaataa tccaccaaca aatcgtgcaa atactgatta ttcttgttta
      421 atcgtgccat acaaccacaa attatcatgg cgtcatcatc agaacactta ggacgtgatt
      481 tcactttagt cgggattaat cctttaaaac cagcagctat cgagggccat gttacatctt
      541 catgattatt tgctgcccat gccccccatc gctcaagaac catctggata tcacgcgcca
      601 tcgttaccac ctctgatttc gtaaatcttc acgcccagcc gtccacctgg cacaggctga
      661 ccgcgcacaa tattgatttc atcaaactgc tcgtcatcaa tgagcacttc cgcatgcgtc
      721 agcgcatcca gcggtgcttt cagaatgttg tccaggtcac ggcggcgctt atccggtggt
      781 tctgcaataa tttttattgc cagccgtccg gacaggctta atttcagccg ctgctggcga
      841 acaataagcg ccacagcccg gcgataacgc tcaccggctt ttgatacaaa atatgtgctg
      901 ccacgacgtc gccagtaggt gttcaccgtc ggcgggtaag gcaaaacaaa ctctatacgc
      961 atcagtaacc tcttttaccc gagcacgccg gttgcaaaag cgtgatcaag aaaacgaaaa
     1021 attaaatcaa cctgggaacc atgcttttct tcgaacgcca gcggatccgc atgaagctcg
     1081 ttgtgatgct cccgacacag cggtagcgtg aaaatatcgt gggattttgt tcccattcca
     1141 ccctgaccat gaccaatcag atgatgagga tcgtccgctg gcttaccaca acacgcacac
     1201 ggctgtgtct ttacccagcg tgtgtatttc tcatttaccc agcggcgacg tttaggtcgt
     1261 ttcatgaagg att
//
LOCUS       DBFIFI010000433         1256 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.439, whole genome shotgun sequence.
ACCESSION   DBFIFI010000433 DBFIFI010000000
VERSION     DBFIFI010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.439"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            219..929
                     /locus_tag="N7B39_005026"
     CDS             219..929
                     /locus_tag="N7B39_005026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HGU2424802.1"
                     /translation="MQTTRTPYSISFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSNLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDIPHAATGDISF
                     QTINDYGALTPRIKATMQ"
     gene            971..>1256
                     /locus_tag="N7B39_005027"
     CDS             971..>1256
                     /locus_tag="N7B39_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HGU2424803.1"
                     /translation="MTAFRAAFKAYRMHQVLLLPRFARLTIALSLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMTSRDITFITDD"
BASE COUNT          364 a          293 c          244 g          355 t
ORIGIN      
        1 taatatctaa acgttcaata aacaacagta gttaaactta ctgttattaa aaatatttac
       61 tttttagcaa ggttttccta ttaaaagaga accaaccttt attacaaacg cattaagtct
      121 ggaaacccgg tgactcgcca atgtattagc aacatttacc gattgtctga tgtaacggtt
      181 ttcccgctta tcctcttaac acatggagaa tgcatcagat gcaaactacg cgtaccccct
      241 actctatttc ctttatggcg acagtgttac ttttattgct ctttgcttgc catagcaccg
      301 ttgccaacgc agctgtcgca ctgggtgcga cgcgggtaat ttatccggca aaccagaaac
      361 aggttttatt gcctgtcacg aataatgatc ctgccagtgt ctatctcatt cagtcatgga
      421 tagaaaatgc tggtgaccaa aaagacaccc agtttgtcat taccccacct cttttttcca
      481 tgcaaggtaa aaaagagaat acgctgcgga taatcaacgc cactaatcat cagttacccg
      541 gcgaccgtga aagcctgttc tgggttaatg ttaaagctat tcctgctatg gaaaaagacc
      601 agaagaatga gaatacatta cagctggcaa ttattagccg cataaaaatg ttttatcggc
      661 ccacgaacct ggcaatggca cctgaagaag cgcctgcaat gcttcggttt cgtcgcagcg
      721 gtagcaatct cactcttatt aatccaacgc cctacttcat taccgtaaca aatatgaaag
      781 caggaaatag caatctccca aacaccatgg tgccgccaaa aggtgaagtg tcggtggata
      841 tcccgcatgc tgccaccggg gatatcagtt ttcagacgat caatgattac ggcgcattaa
      901 ctccgcgtat taaggcaacc atgcagtaag ccagtgacct aattaataat aaactcaatc
      961 ggaattaata atgacagcct ttcgtgccgc atttaaggca tatcgtatgc atcaggtgtt
     1021 actactgcct cgctttgcac ggcttaccat tgcccttagc ttagcaactg cggtatttcc
     1081 cgttgacgca gagtattatt ttaatccacg gtttttaagc aatgatcttg ctgaatctgt
     1141 cgatttatcc gcctttacta agggacgcga ggcaccacct ggtacatatc gggtcgacat
     1201 ttatttgaac gacgaattta tgactagtcg ggatattact tttattacag atgata
//
LOCUS       DBFIFI010000434         1251 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.440, whole genome shotgun sequence.
ACCESSION   DBFIFI010000434 DBFIFI010000000
VERSION     DBFIFI010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.440"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(26..101)
                     /locus_tag="N7B39_005028"
     tRNA            complement(26..101)
                     /locus_tag="N7B39_005028"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(66..68),aa:Asn,seq:gtt)
     gene            complement(202..999)
                     /gene="mtfA"
                     /locus_tag="N7B39_005029"
     CDS             complement(202..999)
                     /gene="mtfA"
                     /locus_tag="N7B39_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HGU2424804.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            1093..1182
                     /locus_tag="N7B39_005030"
     tRNA            1093..1182
                     /locus_tag="N7B39_005030"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1127..1129,aa:Ser,seq:cga)
BASE COUNT          373 a          295 c          273 g          310 t
ORIGIN      
        1 gctaaaaaag cgaattttta gaatttggct cctctgactg gactcgaacc agtgacatac
       61 ggattaacag tccgccgttc taccgactga actacagagg aatcgtgtga acggggcgca
      121 tattacttag cggtaccttg tctgtcaaca ctaaatttca taagtcattt caattggtta
      181 attaatctgc aaagttgtta attaatgaac attcgtcgcc gaaaacgagt ctgtatcatt
      241 agcgtgatgc agtctctgca aaggatcttg ttgataaaat tggcagaaac gttgccacaa
      301 tgaagggaaa cgaggagcaa aaagttctgg ggcgctaaag aaatattcag aaagtacggc
      361 aaaacattca gcaggatcac tggcagcata agcatcaatg ctcgccgcat tctcgccaac
      421 caattcgatt tcttcctgaa tgttgttcat tgcagcatga agatcgtgtt cccagccagc
      481 aacctcacgc aacggaataa agggaactcc gctggcgcga tcgccgttac gggtgtccag
      541 cttatgagcg acttcatgaa taatcaggtt aaaaccagaa gcatcaaaag aatcttgtat
      601 atccaaccag ttcaaaacga taggcccttg ctgccagctc tgacctgact gaacaatacg
      661 ttggttatgc accagaccga tatcgtcttc ccattcatca tcgaccacaa atggcgcagg
      721 ataaattaag acttcatgaa aaccatccag ccattccagt cctaactcca gaacgggtag
      781 gcaaaataga agtgctatcc ggcagcttct taatgaatcc agttcaaagc cctgtaaagg
      841 aacaagccgc ttttgctgta aaaaacgttc ggcaagagtg actaatttgc tttgttcctg
      901 ttccgtcaga cacgttaaaa gggggatcga tagtgcttcc tgccagggaa gggcagtttg
      961 atgtgctgat tcttgtactt tccagggcca cttaatcatc gttttgctcg caaactcgtc
     1021 acttgaacaa aattgcacgg acagggactg ttaaaatgcc aaatttcctg gcatcatggc
     1081 aaccatctga acggagagat gccggagcgg ctgaacggac cggtctcgaa aaccggagtg
     1141 ggggcaactc caccgggggt tcaaatcccc ctctctccgc caaaattcaa tcacttacac
     1201 atcattaagt cagtgacaaa aatcacactt ggaattactt ggaatatttt c
//
LOCUS       DBFIFI010000435         1245 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.441, whole genome shotgun sequence.
ACCESSION   DBFIFI010000435 DBFIFI010000000
VERSION     DBFIFI010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.441"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..107
                     /locus_tag="N7B39_005031"
     CDS             <1..107
                     /locus_tag="N7B39_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HGU2424805.1"
                     /translation="QKWERVCAALRELNKHRDIVRQITDSSRRVVSEK"
     gene            123..536
                     /locus_tag="N7B39_005032"
     CDS             123..536
                     /locus_tag="N7B39_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HGU2424806.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRRSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPVVVDPDLIWSLPDG
                     EIRRYDRQLNIICSECRNSEVMQRVLIFYTGVMME"
     gene            complement(888..>1245)
                     /locus_tag="N7B39_005033"
     CDS             complement(888..>1245)
                     /locus_tag="N7B39_005033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU2424807.1"
                     /translation="WGHSLGAIFVLDCLKNNSCFNYYYISAPSLLWQNERIIKIIKDD
                     ISESKHTKSICLLNGNLSLDYSASLYPEAIKAESVLRNILTEKYSNFSIVQFPELNHQ
                     ETFSAALWNSIIHFSI"
BASE COUNT          400 a          198 c          295 g          352 t
ORIGIN      
        1 ttcagaagtg ggagcgagtc tgcgccgcgc tgcgggagct gaacaagcac cgggatattg
       61 ttcgacagat tactgattct tcccgccgtg ttgtatcgga aaagtgattg ccggaggcgc
      121 ttatggcaaa agtatttaca caggaagagc gggaaaaaat taaagggcag gttgttgagc
      181 tagtacgccg gagtgggcgc gagacgttac ggcaactgga agccaagaca ggtgcgacaa
      241 gatatctgat gagcgttctt gccagagagc tggttgccag tggcgatgta tacaactctg
      301 gttacgggtt attcccatct gaacaggctc gtaaggactg gcaaaacgcc cgcaaaaaac
      361 tatcgagggc aaaactgaag aaaccggttg tggttgatcc tgaccttatc tggtcattac
      421 cagacggaga aatacgccgc tatgacaggc aattgaacat aatttgtagt gagtgccgta
      481 acagtgaagt gatgcagcga gttttgatat tttacacagg agtaatgatg gaatagtgaa
      541 aataacatga aacctttgga ttctggtgtt tcagtggatg ggaaaagagc agatacatag
      601 aaaatgaata gcaataattc acagtctgga tgttgtttgt atgcttataa aatgatccta
      661 gtatgcattg ccggagttga atcagctcca atgaagaaac tgagaggagt atttagaaga
      721 tagctaaatg gatgtaatta ttagtatcta aaaaatagta tttttgaaat gggtctaaga
      781 acacaacaat gtacaaatga gaagttatct tttaatatgt tcaaagaccc attaggattg
      841 atatcagtta tgttagctag attgataaag tgattacata ttttctgtta tatgctgaaa
      901 tgaataatgc tattccagag tgcagcagag aaagtctcct gatgattgag ctctggaaat
      961 tgtacaatag aaaaattact gtatttttct gtcaaaatat ttctcaaaac agattccgct
     1021 ttgatggctt caggatataa agatgcggag taatcaaggc taagatttcc gttaagcaag
     1081 catatacttt tagtgtgctt tgattctgat atatcatctt taattatctt gatgattctt
     1141 tcattctgcc atagaagtga tggtgcagat atataataat agttaaagca tgaattattt
     1201 tttaagcaat caagcacaaa aatagcacct agcgagtgcc cccat
//
LOCUS       DBFIFI010000436         1243 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.442, whole genome shotgun sequence.
ACCESSION   DBFIFI010000436 DBFIFI010000000
VERSION     DBFIFI010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.442"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            42..248
                     /gene="ybcW"
                     /locus_tag="N7B39_005034"
     CDS             42..248
                     /gene="ybcW"
                     /locus_tag="N7B39_005034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbcW"
                     /protein_id="HGU2424808.1"
                     /translation="MNKEQSADDPSVDLIRVKNMLNSTISMSYPDVVIACIEHKVSLE
                     AFRAIEAALVKHDNNMKDYSLVVD"
     gene            complement(413..607)
                     /locus_tag="N7B39_005035"
     CDS             complement(413..607)
                     /locus_tag="N7B39_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HGU2424809.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALDQKGSGNFSAWVIEA
                     CRRRLTSEKRAYTSIKSDEE"
     gene            1002..>1243
                     /locus_tag="N7B39_005036"
     CDS             1002..>1243
                     /locus_tag="N7B39_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424810.1"
                     /translation="MATQTEVARHLSLTDRQLRRLQKLPGAPISNKRGQLDLDAWRDF
                     YISYLRRSKNDVPDGDSEDDYEEKLLIARWELTAEQA"
BASE COUNT          336 a          233 c          310 g          364 t
ORIGIN      
        1 ttttaaccgc tagatgaaga gcaagcgcat ggagcgacaa aatgaataaa gaacaatctg
       61 ctgatgatcc ctccgtggat ctgattcgtg taaaaaatat gcttaatagc accatttcta
      121 tgagttaccc tgatgttgta attgcatgta tagaacataa ggtgtctctg gaagcattca
      181 gggcaattga ggcagcgttg gtgaagcacg ataataatat gaaggattat tccctggtgg
      241 ttgactgatc accataactg ctaatcattc aaactactta acctgtgaca gagccaacac
      301 gcagtctgtc actgtcagga aagtggtaaa actgcaactc aattactgca atgccctcgt
      361 aattaagtga atttacaata tcgtcctgtt cggagggaag aacgcgggat gttcattctt
      421 catcactttt aattgatgta tatgctctct tttctgacgt tagcctccga cggcaggctt
      481 caatgaccca ggctgagaaa tttccggacc ctttttgatc aagagcgatg ttaatttgtt
      541 caatcatttg gttaggaaag cggatgttgc gggttgttgt tctgcgggtt ctgttcttcg
      601 ttgacatgag gttgccccgt attcagtgtc gctgatttgt attgtctgaa gttgttttta
      661 cgttaagttg atgcagatca attaatacga tacctgcgtc ataattaatt atttgacgtg
      721 gtttgatggc gtagatgcac gttgtgacat gtagatgata attattatca ttttgcgggt
      781 cctttccggc gatccgacag gttacggggc ggcgacctcg cgggttttcg ctatttatga
      841 aaattttccg ggatccatgt ccggtttctc ttcaagttaa ctatatgaaa aatataaaaa
      901 caggtcttct gtgaaccgga catgaacaaa aaacagacat gtaaaccgga catgaccggt
      961 tttgttgtga ttgtgaggtg agagtttttg cgaggtgagg agtggctacg cagactgaag
     1021 ttgccaggca tttaagtctg accgatcgcc agcttcgcag attgcagaaa ttgccgggtg
     1081 ccccgatatc gaataagcga gggcaactgg atctggatgc ctggcgcgat ttttacatat
     1141 cgtatctgag gagaagtaaa aacgatgtgc ctgatggcga tagcgaagac gactatgagg
     1201 agaaattgct tattgccaga tgggaactga cagcagaaca ggc
//
LOCUS       DBFIFI010000437         1242 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.443, whole genome shotgun sequence.
ACCESSION   DBFIFI010000437 DBFIFI010000000
VERSION     DBFIFI010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.443"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            30..644
                     /gene="evgA"
                     /locus_tag="N7B39_005037"
     CDS             30..644
                     /gene="evgA"
                     /locus_tag="N7B39_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HGU2424811.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            649..>1242
                     /locus_tag="N7B39_005038"
     CDS             649..>1242
                     /locus_tag="N7B39_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HGU2424812.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPLRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQAL"
BASE COUNT          394 a          258 c          242 g          348 t
ORIGIN      
        1 gagatgcttc attgcaaagg gaataatcta tgaacgcaat aattattgat gaccatcctc
       61 ttgctatcgc agcaattcgt aatttattga tcaaaaacga tattgaaatc ttagcagagt
      121 tgactgaagg cggaagtgcc gttcagcggg tggaaacact taagcctgat atcgtcatca
      181 ttgatgtcga tatccccgga gttaacggta tccaggtgtt agaaacgctg aggaagcgcc
      241 aatatagcgg aattattatt atcgtctccg ctaaaaatga ccatttttac gggaaacatt
      301 gtgctgatgc tggcgctaat ggtttcgtga gtaaaaaaga aggcatgaac aatatcattg
      361 cggctattga agctgcaaaa aatggctact gctatttccc cttctctctc aaccggtttg
      421 ttggaagttt aacgtccgac cagcaaaaac tcgactcctt atcgaaacaa gaaattagtg
      481 tcatgcggta tattcttgat ggcaaggata ataatgacat tgctgaaaaa atgttcatca
      541 gcaacaaaac tgtcagcact tataaaagtc gcctgatgga aaaattagaa tgtaaatcac
      601 tgatggatct ttacacattc gcacaacgta acaaaatcgg ctaaccacat gaagttttta
      661 ccctatattt ttcttctctg ttgtggtctt tggtcgacca taagtttcgc agacgaagat
      721 tacatcgaat atcgtggcat cagtagtaac aaccgtgtca cacttgatcc actacgtctg
      781 agcaacaagg aattacgttg gttagcgagc aaaaaaaatc ttgtgattgc agtacacaag
      841 tcccaaacgg ctacgttgtt gcataccgat tcgcagcaac gggttcgtgg tattaatgct
      901 gattatttaa atcttttaaa aagagcgtta aatatcaaat taacactccg ggaatacgca
      961 gatcatcaaa aagcaatgga cgcgctggaa gaaggtgaag tcgatatagt gttatcacat
     1021 ttagttgctt cgccgcctct taatgatgac attgctgcaa ccaaaccact gataattacc
     1081 tttccggcgc tggtcaccac ccttcacgat tcaatgcgac cgcttacctc atcaaaacca
     1141 gtaaatattg ctcgagtagc aaattatccc cctgacgagg taattcatca atcatttcca
     1201 aaagcaacaa ttatctcttt tacaaattta tatcaggcat ta
//
LOCUS       DBFIFI010000438         1234 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.444, whole genome shotgun sequence.
ACCESSION   DBFIFI010000438 DBFIFI010000000
VERSION     DBFIFI010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.444"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            195..374
                     /locus_tag="N7B39_005039"
     CDS             195..374
                     /locus_tag="N7B39_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HGU2424813.1"
                     /translation="MTFIHQLMLYFCTVVCVLYLLSGGYRAMRDFWRRQIDKRAAEKI
                     SASQSAGSKPEEPLI"
     gene            415..660
                     /locus_tag="N7B39_005040"
     CDS             415..660
                     /locus_tag="N7B39_005040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HGU2424814.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVRSALKQKLRQNLESRLDAEVDA
                     ILDELLGAPAVPEPEGIAGEGSASDGGEPTPDSDMMM"
     gene            737..952
                     /locus_tag="N7B39_005041"
     CDS             737..952
                     /locus_tag="N7B39_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HGU2424815.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            957..>1234
                     /locus_tag="N7B39_005042"
     CDS             957..>1234
                     /locus_tag="N7B39_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HGU2424816.1"
                     /translation="MSRKLRYGLSAAVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVEKN"
BASE COUNT          304 a          278 c          370 g          282 t
ORIGIN      
        1 cccggtgctg attgacagca ttgcggtgga agtgaacaac gcggatgccg cggcatgata
       61 aaaaaagccg ccagttgcct gagacctctg gcggtgtgag attcatggag aatcaaggaa
      121 agatactacc actttcgtca tcggcatttt taaatgaaaa ctgtttactt agtcaaccat
      181 aacggtaaga aattatgaca tttattcatc agctgatgct gtacttctgt acggtggtct
      241 gtgtgctgta tctcctttcg ggtgggtaca gggccatgcg tgacttctgg cgcagacaga
      301 ttgacaaaag ggccgctgag aaaatcagcg ccagtcagtc agccggaagc aaacccgaag
      361 agccgctcat ttagcggcaa ctttcttaat cacatctttc gacgagaaaa tcccatgtca
      421 gaaattacat ccctggtcac tgctgaggca gtgaaggaag tcctgcgctc tgaagaagtc
      481 cggagtgcac tgaaacagaa actccgccag aatcttgagt cgcgtcttga tgcagaagtg
      541 gatgctattc tggatgaact gctcggcgca ccggcagttc ctgagccgga aggtatcgcg
      601 ggtgagggga gtgcttcaga tggcggtgaa cccacacctg acagcgacat gatgatgtaa
      661 gcatgcgcaa ggggccatcg gtgtgtgccg gtggtcttta tattgttgtg agcttccgga
      721 ttgcgggaga cggggtatgt accagatgga aaaaatcaca acaggtgtgt catacaccac
      781 gtcagcggtg ggaacgggct actggttcct gcagttgctg gacagggttt ccccgtctca
      841 gtgggcggca ataggcgtgc tggggagtct gctgtttggg ctgctgacat atctgacgaa
      901 cctgtatttc aagattaaag aagaccggcg taaggcggca cggggagagt aagctgatga
      961 gcaggaaact ccgctatggt ttatcggctg ccgttctggc gctgattgcc gcaggtgctt
     1021 ctgcgcctga aatcctcgac cagtttctgg atgaaaagga aggtaaccac accacagcat
     1081 accgtgatgg tgcgggtatc tggaccatct gccgtggagc cacccgggtg gatggtaagc
     1141 ctgttattcc tggcatgaag ctgtcgaagg aaaaatgcga ccgggttaac gccattgagc
     1201 gtgataaggc gctggcatgg gtggagaaaa acat
//
LOCUS       DBFIFI010000439         1220 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.445, whole genome shotgun sequence.
ACCESSION   DBFIFI010000439 DBFIFI010000000
VERSION     DBFIFI010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.445"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(23..139)
                     /locus_tag="N7B39_005043"
                     /pseudo
     CDS             complement(23..139)
                     /locus_tag="N7B39_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            408..>1220
                     /locus_tag="N7B39_005044"
     CDS             408..>1220
                     /locus_tag="N7B39_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HGU2424817.1"
                     /translation="MAFKHYDVVRAASPSDLAEKLTHKLKEGWQPYGGPVAITPYTLM
                     QAVAIEGDPQVGPSSKPDWFYVVVLAGQSNGMAYGEGLPLPDSYDAPDPRIKQLARRS
                     TVTPGGESCTYNDIIPADHCLHDVQDMSTLNHPKADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGAEGTFSADTGASQDSARWGVGKPLYQDLIARTKAAL
                     QKNPKNVLLAVCWMQGEFDMSAATHAQQPALFTAMLTQFRADLSVFNAQCHGG"
BASE COUNT          281 a          319 c          355 g          265 t
ORIGIN      
        1 ggggagtttg ttaaaagaaa ctggcatggt gaatccccct gtgcggaggg gcaatcagcg
       61 agtaggtata tgggataatc gcggattcag gtgctggtac tgaattcacc gggaggcacc
      121 cggcaccatg caatggcaca tagcgccact ctccagcccc tctccggagg ggctttctta
      181 tggacaaaaa aagcccgcgc agggagacgc gggcggcaag gaataaacaa caaaacgtga
      241 agtaatattt cagctggcga ataatatccg acagtaatca ctctgcgcaa tagcgcggcc
      301 tttttcgtat tgcgggctgt tgtctctctt ctgccattgt cctgtaactt ccggacttca
      361 gcccgctcct cattttactc acaatattat cccggccggg aggattcatg gcatttaaac
      421 actatgatgt tgtcagggcg gcgtcgccgt cagaccttgc ggaaaagctg acacacaaac
      481 tgaaagaggg ctggcagcca tacggcggac cggttgccat tacgccgtac acactgatgc
      541 aggcggtggc tattgaagga gatccacagg tcggcccttc atctaagccg gactggttct
      601 acgtggttgt gcttgccgga cagtccaacg gcatggccta cggtgaaggg cttccgttac
      661 cggattctta cgatgctccg gatccgcgca ttaaacagct ggcgcgccgc agcacggtaa
      721 ctccgggtgg agagagttgt acgtataacg acatcattcc ggccgaccac tgcctgcatg
      781 atgtgcagga tatgagtacg ctgaatcatc cgaaggcaga cctgagcaaa gggcagtacg
      841 gctgtgtcgg ccagggctta catattgcca aaaaactgct tccgtatatc ccgaataacg
      901 cggggatcct gctggtacca tgctgtcgtg gtggttctgc attcacccag ggcgctgagg
      961 ggacattcag tgcggacacg ggggccagcc aggattcggc acgctggggt gtgggtaaac
     1021 cgttatatca ggacctgatt gcgcgcacta aagctgcatt acagaagaac ccgaaaaatg
     1081 tgttgctggc ggtgtgctgg atgcagggag agtttgacat gagcgccgcc acccacgcac
     1141 agcaacctgc gctgtttaca gccatgctga cacagtttcg tgctgacctc tccgtgttta
     1201 acgcgcagtg ccatggtggc
//
LOCUS       DBFIFI010000440         1211 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.446, whole genome shotgun sequence.
ACCESSION   DBFIFI010000440 DBFIFI010000000
VERSION     DBFIFI010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.446"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            158..793
                     /locus_tag="N7B39_005045"
     CDS             158..793
                     /locus_tag="N7B39_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HGU2424818.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AAERISEQGLYAMRDVQVARLALFHGDPEKAKKLTNEASALLSDDSTEWAKFAKPGKK
                     TNVNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVM
                     ENQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT          386 a          250 c          251 g          324 t
ORIGIN      
        1 catattccta caattgtaag actaaaatac ttcttgcgat aataactaca actgtaagat
       61 aaccctttca aaatgaccgt tgctctctga tttctcattt catgctcacc caatatgatg
      121 gcggcgtttt ctaaaactgt taaagaatga ggtaagtatg aaacgtttaa ttatggctac
      181 gatggtcaca gctattctgg catcttcaac tgtatgggct gctgataatg ctccggtagc
      241 ggcacaacag caaactcagc aagttcagca aacccagaaa acggcagctg cagcagagcg
      301 tatctctgaa caaggtttat atgcgatgcg tgacgtgcag gtagcgcgcc tggctctgtt
      361 tcatggcgat ccggaaaagg caaaaaaact gaccaatgaa gcttcagctt tgttgtcaga
      421 tgacagtacc gaatgggcaa agttcgctaa gccaggtaaa aagaccaatg tcaatgatga
      481 ccaatatatt gtcattaatg catctgtcgg aatttctgag agttatgtag cgacacccga
      541 aaaagaagcc gcgataaaaa ttgccaatga aaaaatggcg aaaggggata aaaagggcgc
      601 aatggaagag cttcgcctgg ccggtgttgg tgtaatggaa aaccaatatc tgatgccgct
      661 taaacagact cgtaatgcac ttgctgatgc gcagaaactg cttgataaaa agcaatacta
      721 cgaggcaaac ctggcgctta aaggtgcgga agatggaatc atcgtcgata gcgaagcact
      781 gtttgtgaac taatgctgac aggcagtata ttgaggtaaa ggatttaact ccctccagcc
      841 gacaattttt tatgaccaca atacgacagg ttgtggtcat aaaattattt ggcgctcgct
      901 gttctataaa ctcgcatttt atattcctca aagtaaaccg caataatccc gtgtcatcac
      961 aaatcattta atatgttata ttttatggta cgctctaaaa agcgtaatca tacccttact
     1021 tgtaaatcac cggcgcttct tacagttgta agaataacat cacaaatgtc gcgactctct
     1081 ctgattacca cgaaccaata tttatcatat tcttcttttc gtcctcacta cttaccatgc
     1141 agaaaggaat tggaagtagc gatgaaatga ttttcagcac ttgatacagg aatttaaaaa
     1201 tatcgagtgc c
//
LOCUS       DBFIFI010000441         1190 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.447, whole genome shotgun sequence.
ACCESSION   DBFIFI010000441 DBFIFI010000000
VERSION     DBFIFI010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.447"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            126..1171
                     /locus_tag="N7B39_005046"
                     /pseudo
     CDS             126..1171
                     /locus_tag="N7B39_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
BASE COUNT          285 a          277 c          334 g          294 t
ORIGIN      
        1 ggtgattgtt ggtatgacat gaataaaagg tttttcgttt gactggaaaa ttatgttgca
       61 ggaacgagag gactgaggaa caaagcttat tccggcgctg tccgtccttg ctgtcactga
      121 cactgatgtc tgctttatta tcgcctttat ctcttcaggc agcggatgtc cggcgtagcg
      181 gagatgaagc atttatcatt cagcagcagc gtcaggaagc ccttgagcaa caactgacgc
      241 cttcagcccc tgatgttcgc ctttctgcac ctggctcttt tgcccataag attaattttc
      301 ctgttgaaac gccctgtttt cagattaaac agacggaact gaagggggct gatgcgttac
      361 cacactggct gcctttacaa aaaatcgcca acggggcggt cgggcattgc ctgggggcga
      421 aaggaattaa tctgctgatg agtacattgc agaaccgtct ggtcgatcat ggttatgtca
      481 ccacccgtgt tctggcacct tcgcaggatt taaaaagcgg tatcctccgg ctggttatta
      541 ttcccggtgt tgtgcgacat gtgcgtctga caccggacag tgatgactat attcagttgt
      601 attcctcatt cccggcacac gaaggttctc tgctggattt acgggacatt gagcaggggc
      661 tggatttagg taacagccgg atacagggac aacatactga gctgaatgca accagtggaa
      721 atctgtctac acagaatgcg caactgagtg ccgatacgct ttccgcccgg actgccgggc
      781 agttcagcag taatggcggt acgataaatg ccgacacact gcagatatcg gcacaaagcc
      841 tgtcaaatcg taaaggcagt ctgattcaga cgggaacagg ggatttttcg ctgagtctgc
      901 cgggaagcgt ggataaccgg gaagggctgc ttgcggcaaa tgggcggtgc gtctggatgc
      961 actgagcctt gataatcgca aggggaaagt gcaggcggag cagtcaccct cccttcagaa
     1021 atccccgccc acgtttctga aaccgtttgt ggctggtgtc tgtgcggcat tgctggcggt
     1081 cagcgtggct attccgggat ggcagtttct gacacagcca tcaccggagg agcagcattt
     1141 tacctggggg aatggttgta aaaagcagtg aaaacagtaa atacagagta
//
LOCUS       DBFIFI010000442         1184 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.448, whole genome shotgun sequence.
ACCESSION   DBFIFI010000442 DBFIFI010000000
VERSION     DBFIFI010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.448"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..527
                     /locus_tag="N7B39_005047"
     CDS             <1..527
                     /locus_tag="N7B39_005047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HGU2424819.1"
                     /translation="CDFRDLVVLRFDSVNVRVVYLNGDPWFVAKDVCVALEISNSRDA
                     LKALDADEKKTVALSYGIRGNPNHSLISESGFYKLIARSRKAVTPGTFAHRFSNWVFR
                     NVIPGIRKTGAYGIPWGALQDFSRRKEQYQLSASQKGRELQACKRKKRELEEEEKRLI
                     REYQPEFYFGNRIQ"
     gene            complement(648..827)
                     /locus_tag="N7B39_005048"
     CDS             complement(648..827)
                     /locus_tag="N7B39_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HGU2424820.1"
                     /translation="MERDHINNKSQKLQARAPHEVVEAMEQVKETGESTAQFIVTAMR
                     GEIKRRQRRKAKEAE"
     gene            1024..>1184
                     /locus_tag="N7B39_005049"
     CDS             1024..>1184
                     /locus_tag="N7B39_005049"
                     /inference="COORDINATES: protein motif:HMM:NF033153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HGU2424821.1"
                     /translation="MRNHTTHPQGRDLHNLNKFTWRFIALSTAQPRVITIEATSEQEA
                     RQQSPVGCV"
BASE COUNT          310 a          269 c          301 g          304 t
ORIGIN      
        1 tctgtgattt tcgtgatctg gtcgttctgc gctttgatag tgtgaacgtt cgcgtggtgt
       61 atctgaacgg cgatccgtgg tttgttgcaa aggatgtttg tgttgctctg gaaataagta
      121 actcacgcga tgctctaaaa gcactagatg cggatgaaaa gaaaaccgtc gctttaagct
      181 acggtattcg aggaaatcca aatcatagcc tcatttctga atccggtttt tacaagctga
      241 tcgccagaag tcggaaagct gttactcctg gcacgttcgc ccatcgtttc agtaactggg
      301 tattcaggaa tgtgatacca ggaatcagaa aaacgggggc ttatggtatc ccgtggggcg
      361 cattacagga cttttctcgc cgtaaagagc aatatcagtt aagtgccagt cagaagggga
      421 gggagctaca ggcatgtaag cgcaaaaagc gtgagctgga ggaagaagaa aaaaggctga
      481 tacgtgaata tcagcctgag ttttactttg gcaaccgcat tcagtgacaa aacaaaggcg
      541 acccggatca gaggtcgcca atgggaaaac actaaacata agcccaaaca tcatagcgat
      601 ttgttggctg gtgggcaatg tgatcagtgt gctggcttgc tggtggacta ttccgcttct
      661 ttggccttgc ggcgctggcg gcgtttgatc tcgcctcgca tggctgtaac gataaattgc
      721 gcagtgctct cgcctgtttc ttttacttgc tccatagcct caacaacttc atgcggggcg
      781 cgagcctgta atttctgtga tttgttattg atatggtctc tttccatttc tggttccctt
      841 tgtgattact ggaatccagt atacacgaaa aagaccaaaa caaaacgctt gaagtggatt
      901 ccacttagtg attatactgg aatccagttg agtgtttatc actcaatgca aacgaggctg
      961 aaaaggattg ccgtcctttc gcagcctctg accaccaacg atagcaacag tatcgaggta
     1021 gctatgagaa atcataccac acacccgcaa gggcgggact tgcacaacct gaataaattc
     1081 acctggcgtt ttatcgccct gagcacggca caaccgcgcg tgattaccat tgaggccacc
     1141 agcgaacagg aagcacgcca gcaatcccca gttggctgcg tgat
//
LOCUS       DBFIFI010000443         1180 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.449, whole genome shotgun sequence.
ACCESSION   DBFIFI010000443 DBFIFI010000000
VERSION     DBFIFI010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.449"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..253
                     /locus_tag="N7B39_005050"
     CDS             <1..253
                     /locus_tag="N7B39_005050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HGU2424822.1"
                     /translation="SGAVIRGVFDDPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRG
                     DTLTINGEMFWVDRVSPDDGGSCYLWLNRGQPPAVNRRR"
     gene            268..801
                     /locus_tag="N7B39_005051"
     CDS             268..801
                     /locus_tag="N7B39_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU2424823.1"
                     /translation="MAIKGLDQAIENLSRVRKNAIPAASAMAINRVATTAINQSSSQV
                     ARETKVRRKLVKERSRLKRATVRNPNARIIVKRGDLPVIKLGIRMLGRRPDSILKAGQ
                     HRYQRAFIQRLKNGRWHVMQRVAGKNRYPIDVVKIPMAAPLKQAFDENIDRIRRERLP
                     GELAYALKQQLRIAIKR"
     gene            798..>1180
                     /locus_tag="N7B39_005052"
     CDS             798..>1180
                     /locus_tag="N7B39_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HGU2424824.1"
                     /translation="MKHTDIRAAVLDALELHEHGATLFDGRPVVFDEEDFPAVAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDQWMESRIYPAVTAIPALADLITTM
                     VTQGYEYRRDDDMALWSSADLTYSIT"
BASE COUNT          280 a          274 c          359 g          267 t
ORIGIN      
        1 gtccggcgca gtaatacgtg gtgtttttga tgaccctgaa aatatcagct atgccggaca
       61 gggcgtgcgc gttgaaggct ccagcccgtc cctgtttgtc cggactgatg atgtgcggca
      121 actgcggcgt ggtgacacgc tgaccattaa cggcgagatg ttctgggtgg accgtgtttc
      181 tccggatgac ggagggagtt gttatctctg gctcaaccgt gggcaaccac cggcagttaa
      241 ccggcgacga taaacgcagg gtgaattatg gcgataaaag ggcttgatca ggcgattgaa
      301 aatctgagcc gggttcgtaa aaacgccatt ccggcggctt cagcaatggc cattaaccgc
      361 gtggccacaa cggcgattaa tcagtcttca tcacaggttg cccgggagac aaaggtacgc
      421 cggaaactgg taaaggaacg gtccagactg aaacgggcca cggtcagaaa tccgaatgcc
      481 agaattatcg ttaaacgcgg tgatctccct gtgattaagc tggggatcag gatgctgggc
      541 cgtcgtccgg acagcatact taaagctggt cagcatcggt atcagcgggc atttatccag
      601 cgattaaaaa atggtcgctg gcatgtcatg cagcgtgtgg ccgggaaaaa ccgttacccc
      661 attgatgtgg tgaaaatccc gatggcggcc ccactgaaac aggcgtttga tgagaatatt
      721 gaccgtatcc ggcgtgaacg tctgcccgga gaactggcat acgcgctgaa acaacaactt
      781 aggattgcga taaaacgatg aaacatactg atatccgtgc ggcagtgctg gatgcactgg
      841 agctgcatga acacggggcg acgctgtttg atggtcgccc cgttgttttt gacgaagagg
      901 attttccggc cgtcgcggtt tatctgacgg atgcagagta taccggtgaa gagctggatg
      961 cagatacctg gcgggccaca ctgcatattg aggtgttttt accggcacag gtaccggatt
     1021 cggagcttga tcagtggatg gaaagccgga tttatccggc ggtgactgcg atcccggcac
     1081 tggcagacct gattacgacg atggttacgc agggctatga gtatcgtcgt gatgacgata
     1141 tggcgttatg gagttctgcg gatctgactt attccattac
//
LOCUS       DBFIFI010000444         1159 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.450, whole genome shotgun sequence.
ACCESSION   DBFIFI010000444 DBFIFI010000000
VERSION     DBFIFI010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.450"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            13..134
                     /locus_tag="N7B39_005053"
                     /pseudo
     CDS             13..134
                     /locus_tag="N7B39_005053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            196..894
                     /gene="nleD"
                     /locus_tag="N7B39_005054"
     CDS             196..894
                     /gene="nleD"
                     /locus_tag="N7B39_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector zinc metalloprotease NleD"
                     /protein_id="HGU2424825.1"
                     /translation="MRPTSLNLVLHQSSRSSSMSDTDIESLVKASSVQWIKNNPQLRF
                     QGTDHNIYQQIEAALDKIGSTETGRVLLNAIESISRLKSETVVIHLNSSRLGVMAHRD
                     IDAENHRGTGSDFHCNLNAVEYPCGEGISVVDFHATIVFHELLHVFHNLNGERLKVES
                     SRAESQKYSPLLLEEARTVGLGAFSEEVLSENKFHEEIGMPRRTSYPHDSALIHDDNT
                     VSLGFQQVRLHPLL"
     gene            complement(991..1083)
                     /locus_tag="N7B39_005055"
                     /pseudo
     CDS             complement(991..1083)
                     /locus_tag="N7B39_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001504497.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          325 a          240 c          273 g          321 t
ORIGIN      
        1 tggagttggt aacaaattgt gtggttggca attcttggcg gatcacggaa atgggcaatg
       61 tcgctgagga ctggcaaatg gctatgatcc gctttattat cgggtttggt gaccgccttg
      121 acggtcactt ttaaaaaaat cattttcaca gagtggtaaa caggctgcta ataagtagca
      181 ttctcaggag tcctgatgcg ccctacgtcc ctcaacttgg tattacatca gtcatcaagg
      241 tcgagctcaa tgtcagatac agatatcgag tctcttgtaa aagcatcgag cgttcaatgg
      301 ataaaaaata atccgcaact tcgtttccag gggactgatc ataatatata tcagcagatt
      361 gaagcagcac tcgataagat tggctctaca gagacagggc gtgtactcct gaatgctatt
      421 gaatcaatat cccgacttaa atcagaaaca gtggtaatac acctcaactc ttccagacta
      481 ggagttatgg cacatagaga tatagatgct gagaaccatc gggggactgg ttccgatttt
      541 cactgtaatc tgaatgcagt tgaatatccc tgtggggagg ggattagcgt ggtggacttt
      601 catgcgacta ttgtttttca tgagttgctc catgttttcc acaatttaaa tggggagcgt
      661 ttgaaagttg agagttcccg agcagaatca caaaaatact ctccactttt actcgaagaa
      721 gccaggactg ttgggttggg ggctttttca gaggaggtgc tttcagaaaa taaattccac
      781 gaagagattg ggatgccccg tagaacctcc tacccgcacg actcagctct tattcatgat
      841 gacaatacag tgagtctggg attccaacag gtaagactgc atccattgct ttagctgtgt
      901 ttcttgtgct gaatatatat tggtggggca aacggaggca cacaaaactt tgcactggat
      961 tgcaaggctt tgtgctattc cgatagtggt tcaggtggag taccccacct tttcatcaag
     1021 ccagcccacc caccactgca tcatttctct gcgcttatag agatactgag catggttgta
     1081 aattcagcaa acgacgtaat gtgtttgaca aaaaattagc gcaagaagac aaaaatcacc
     1141 ttgcgctaat gctctgtta
//
LOCUS       DBFIFI010000445         1158 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.451, whole genome shotgun sequence.
ACCESSION   DBFIFI010000445 DBFIFI010000000
VERSION     DBFIFI010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.451"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..>1158
                     /locus_tag="N7B39_005056"
     CDS             join(<1..285,285..>1158)
                     /locus_tag="N7B39_005056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS1203 family transposase"
                     /protein_id="HGU2424826.1"
                     /translation="NTRFSPEVRQRAIRMVLESQDEYDSQWAAICSIAPKIGCTPETL
                     RVWVRQHERDTGGGDGGLTSAERQRLKELERENRELRRSNDILRQASAYFGEGGVRPP
                     LEKMMPLLDKLREQYGVGPVCSELHIAPSTYYHCQQQRHHPDKRSARAQHDDWLKREI
                     QRVYDENHQVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVLRGKKVRTTISRKA
                     VAAGDRVNRQFVAERPDQLWVADFTYVSTWQGFVYVAFIIDVFAGYIVGWRVSSSMET
                     TFVLDALEQALWARRPSGTIHHSDKGSQYVSLAYTERLKEAGLLASTGSTGDSYDNAM
                     AESINGLYKAEVIHRKSWKNRAEVELATLTWVDWYNNRRLLGRLGHTPPAEA"
     misc_feature    275..391
                     /locus_tag="N7B39_005056"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
BASE COUNT          274 a          277 c          357 g          250 t
ORIGIN      
        1 aaaatactcg tttttccccc gaagtccgtc agcgggcgat tcgtatggtt ctggaaagtc
       61 aggatgaata tgactcacag tgggcggcaa tttgttccat tgccccaaag attggctgta
      121 cgccggagac tctgcgtgtc tgggttcgcc agcatgagcg ggataccggg ggcggtgatg
      181 gtgggctcac cagcgctgaa cgtcagcgtc tgaaagagct ggaacgtgaa aatcgtgaac
      241 tgcgccgcag taacgatatc cttcgccagg cttccgctta ttttgcgaag gcggagttcg
      301 accgcctctg gaaaaaatga tgccactgct ggataagctg cgtgagcagt acggggtcgg
      361 accggtatgc agcgaactgc atattgcccc gtcaacgtat taccattgtc agcaacagcg
      421 acatcatccg gataaacgca gtgcccgtgc gcagcacgac gactggctga agagagagat
      481 acagcgcgta tacgatgaaa atcatcaggt gtacggtgtg cgtaaagtct ggcgtcagtt
      541 gttacgggaa ggaatcaggg tggccagatg tacagtggca cgtctcatgg cggttatggg
      601 acttgccggt gttctccggg gtaaaaaggt ccgtacgacc atcagccgga aagccgttgc
      661 cgcaggcgac cgcgtaaacc gtcagttcgt ggcagaacga cctgaccagc tgtgggtggc
      721 tgattttact tacgtcagca catggcaggg cttcgtctat gtggcgttta tcattgatgt
      781 gtttgccgga tacatcgtgg ggtggcgggt ctcatcgtct atggaaacga cattcgtgct
      841 ggatgcgctg gagcaggcgt tgtgggcccg tcgtccgtct ggcaccatcc atcacagcga
      901 taaaggctct cagtatgtgt cactggccta tacggagcga ctaaaagaag ccggattact
      961 ggcatcaaca gggagtacag gcgactcgta tgacaacgcg atggctgaga gcatcaatgg
     1021 tctttacaaa gcggaggtaa tacaccgtaa gagctggaaa aaccgtgcag aagtggaact
     1081 ggccacacta acgtgggtgg actggtataa caatcgacga ttgctgggaa ggctgggcca
     1141 tactcctccg gcagaagc
//
LOCUS       DBFIFI010000446         1153 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.452, whole genome shotgun sequence.
ACCESSION   DBFIFI010000446 DBFIFI010000000
VERSION     DBFIFI010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.452"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            132..293
                     /locus_tag="N7B39_005057"
                     /pseudo
     CDS             132..293
                     /locus_tag="N7B39_005057"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            277..342
                     /locus_tag="N7B39_005058"
     CDS             277..342
                     /locus_tag="N7B39_005058"
                     /inference="COORDINATES: protein motif:HMM:NF033646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAST1 family heat-stable enterotoxin"
                     /protein_id="HGU2424827.1"
                     /translation="MWCATACASCHGRNTKPSLAT"
     gene            458..715
                     /locus_tag="N7B39_005059"
     CDS             458..715
                     /locus_tag="N7B39_005059"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU2424828.1"
                     /translation="MATFFAYPADIRKVIYTTNAIESLNSVIRHAIRKRKVFPTDESV
                     KKVVWLAIQAASQKWTMPLRDWRMAMSRFIIEFGDRLDGHF"
     gene            complement(762..>1153)
                     /gene="nleF"
                     /locus_tag="N7B39_005060"
     CDS             complement(762..>1153)
                     /gene="nleF"
                     /locus_tag="N7B39_005060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector caspase inhibitor NleF"
                     /protein_id="HGU2424829.1"
                     /translation="ELEEIFQELSAFVSGLMDKRNSVFEVRNENTDEVVGALRAGMTI
                     EDRDSYIRDLFFLHSLKVKIEESRQGKEDSKCKVYNLLCPHHSSELYGDLRAMKCLVE
                     GCSDDFNPFDIIRVPDLTYNKGSLQCG"
BASE COUNT          317 a          284 c          250 g          302 t
ORIGIN      
        1 tagatgatat tagtgacctg tttcagagca ttagcgcaag gcgattcttt gtcttcttgc
       61 actaattttt tatcataaaa atgttcctag cactgggcat caatatcgca ggtcagaaag
      121 agctcctggg gatgcggctg gccgaaaatg aaggggcgaa tttctggttc aatgtgctga
      181 ctgaactgaa aaaccgcggt ctgaacgata tcctcatcgc ctgtgtgtat ggcctgaaag
      241 aattcccgga ggcccgcatc cagttatgca tcgtgcatat ggtgcgcaac agcatgcgct
      301 tcgtgtcatg gaaggaatac aaagccgtca ctcgcgacct gaaagcgatt agcctcccac
      361 agaagaggca ggccagcagg cactggaagc gtttgctgcg gcctgggact gccgctatcc
      421 gcagataagc cggtgctagc tgtcaaactg gactaacttg gcgacgtttt tcgcttatcc
      481 ggcagatatc cgcaaagtga tctacacaac gaacgccatc gagtcgctga acagtgtgat
      541 ccggcatgcc atcaggaaac gcaaggtgtt cccgacggac gagtcagtaa aaaaagtggt
      601 gtggctggca atccaggccg cgtcacagaa atggacaatg ccgttaaggg actggcgaat
      661 ggcaatgagc cgctttatta tcgagtttgg tgaccgtctt gacggtcact tttaagaaaa
      721 ggcatttaca cagaatccta aacaggctca caggcctcta atcatccaca ttgtaaagat
      781 cctttgttgt aagtaagatc tggtacccta ataatatcaa aaggattaaa atcatcactg
      841 catccttcca cgaggcattt cattgctcgt agatcaccat atagctctga agagtgatgc
      901 ggacatagca gattataaac tttacatttc gaatcttctt tgccttgtct actttcctca
      961 atttttactt tcaatgaatg cagaaaaaaa agatccctga tataactatc cctgtcctct
     1021 atcgtcattc ccgccctcag cgctccgaca acctcatcag tattttcatt tctcacctca
     1081 aatacactat ttctcttatc catcagccca cttacaaaag cacttagctc ttgaaaaatt
     1141 tcctcaagct cat
//
LOCUS       DBFIFI010000447         1149 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.453, whole genome shotgun sequence.
ACCESSION   DBFIFI010000447 DBFIFI010000000
VERSION     DBFIFI010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.453"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(5..81)
                     /locus_tag="N7B39_005061"
     tRNA            complement(5..81)
                     /locus_tag="N7B39_005061"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(45..47),aa:Arg,seq:tct)
     gene            complement(95..171)
                     /locus_tag="N7B39_005062"
     tRNA            complement(95..171)
                     /locus_tag="N7B39_005062"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(135..137),aa:Arg,seq:tcg)
     gene            complement(173..248)
                     /locus_tag="N7B39_005063"
     tRNA            complement(173..248)
                     /locus_tag="N7B39_005063"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(213..215),aa:Ile,seq:cat)
     gene            complement(562..1050)
                     /locus_tag="N7B39_005064"
     CDS             complement(562..1050)
                     /locus_tag="N7B39_005064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HGU2424830.1"
                     /translation="MGIRELNLTKEQHEWLNGWLELWGAWVYSGRLEKRMSSVIAKFM
                     ESVEPGRVMTRPMCNDDDGMLISQVVDSVMYIDKKAFGILLSYYAHGSSKHAIASYYH
                     SVARPRKMLCRGGGRIQKPSLATCRREVDEILNASLFMIYPILDSAFKNRKRVEKIKH
                     VA"
     gene            complement(1041..>1149)
                     /locus_tag="N7B39_005065"
     CDS             complement(1041..>1149)
                     /locus_tag="N7B39_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424831.1"
                     /translation="AVKPLKFANQMYIDTGAVFCGNLTLIQVQGEGAWA"
BASE COUNT          302 a          281 c          255 g          311 t
ORIGIN      
        1 agtgtggcgc gttgtactgg attcgaacca gtgaccgatt gcttagaagg caattgctct
       61 gtcctgctga gctaacaacg cagaataccg ataatggacc accaccgggg actcgaacct
      121 cgcgcaacca gcttcgaagg ctgacgctct atcccgatga gctaatggcg gttggtggcc
      181 cttgctggat ttgaaccagc gacctggcga ttatgagtcg ctcgctctca ccactgagct
      241 aaagggccga gcgtaggata ataacgttac gaaatcaatg ttgcaagagt tcacaaattc
      301 actgattcaa aattattctt gtaaaaaatc ttttaacgtc aggcacaaag agcctgtaca
      361 tagatttgtg taattgcctg attttgatat gttcaatcca gcatcaaatg aaggttaatt
      421 tatggacgaa aaacagttac aggctctaat gacctttata cgattgaccc aaataaaaaa
      481 agccaccgtt gcaacttaag agtcactaac ggcagcttat gcgaatagtg ttgctcattt
      541 gctcaacgat gtcaacacgt tctatgctac atgcttaatt ttctctacac gtttccggtt
      601 tttaaacgca ctatccagaa tcgggtaaat cataaacaac gaggcattga ggatttcgtc
      661 aacttcccgt cggcaggttg ctagcgatgg tttttgaatg cgcccgccgc cccggcataa
      721 catcttgcga ggtcttgcga cgctatgata gtaagatgca atggcgtgct tggaagagcc
      781 gtgggcgtag tagctgagga ggatgccaaa ggctttcttg tcaatgtaca tgacggaatc
      841 gacgacctga gaaatcaaca ttccatcatc atcattacac attggccttg tcataactct
      901 tcccggctcc acgctctcca tgaatttagc tattacgctg ctcatgcgct tttccagacg
      961 acctgaataa acccatgcgc cccacagttc aagccagcca ttcagccact catgctgttc
     1021 tttggtgagg tttagttctc ttatgcccac gcgccttctc cctgtacctg aatcaatgtg
     1081 agatttccgc agaacactgc gccggtatcg atatacatct ggttggcaaa tttgagtggt
     1141 ttcactgct
//
LOCUS       DBFIFI010000448         1148 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.454, whole genome shotgun sequence.
ACCESSION   DBFIFI010000448 DBFIFI010000000
VERSION     DBFIFI010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.454"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(64..>1148)
                     /locus_tag="N7B39_005066"
     CDS             complement(64..>1148)
                     /locus_tag="N7B39_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HGU2424832.1"
                     /translation="NFHNLADIPNIDVINGQAEFINNHSLRVHRPEGNLEIHGEKIFI
                     NTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGHLGILGGGYIGVEFASMFANFGSK
                     VTILEAASLFLPREERDIADNIATILRDQGVDIILNAHVERISHHENQVQVHSEHAQL
                     AVDALLIASGRQPATASLHPENAGIAVNERGAIVVDKRLHTTADNIWAMGDVTGGLQF
                     TYISLDDYRIVRDELLGEGKRSTDDRKNVPYSVFMTPPLSRVGMTEEQARESGADIQV
                     VTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGASLLCVDSHEMINIVKMVMDAGLP
                     YSILRDQIFTHPSMSESLNDLFSLVK"
BASE COUNT          304 a          280 c          242 g          322 t
ORIGIN      
        1 ggaattaata agattctttt ctctgagacg ccagaatatt tgttctggcg tctgattttg
       61 agtttatttg actaatgaaa atagatcatt gagtgattcg ctcatcgacg gatgagtaaa
      121 tatctgatcg cgtaatatgc tataaggcag cccggcatcc atcaccattt tcactatatt
      181 aatcatctcg tgggagtcaa cacacagcag tgatgcccct aatatacgtt gggttttatt
      241 atcaacaatc gcttttaata ccccacgggt atcgttcatc actctggcgc gcggaattgc
      301 agctacaggc aatgtcacca cctgaatatc agcaccactc tctctggctt gttcttctgt
      361 cataccaacc ctggacaggg gcggtgtcat aaatacggaa taaggcacat ttttccgatc
      421 atcagtacta cgtttgcctt cacccagtaa ctcatcacgt acaatgcggt aatcatccag
      481 tgatatgtaa gtaaattgca gcccgccggt aacatctccc atcgcccaaa tattgtctgc
      541 ggtggtatgt aatcgcttgt caacgacaat tgccccgcgc tcgtttactg cgataccggc
      601 attttctgga tgtaacgaag cggtagccgg ttgacgaccg gaagctatta acagtgcatc
      661 caccgccagt tgggcgtgct cgctatgcac ttgcacttga ttttcatggt gactgattcg
      721 ctccacatgg gcattgagga taatatcgac gccctgatcg cgtaaaatcg tcgcgatatt
      781 atcagcaata tcccgttctt cccgaggcaa aaacagcgaa gctgcttcta aaatggttac
      841 cttgctgcca aaattagcga acatagaggc gaactcaacg ccaatatatc cgccgcccaa
      901 aatacctaaa tgcccaggca attcttttag attaagtaat ccggtgctgt catatactcc
      961 tggcgtggtg gtaattccag gaattggcgg aaccacggtt tgtgcaccgg tattaataaa
     1021 aattttctcg ccatgaattt ccagatttcc ctcaggccga tgaacacgca ggctatgatt
     1081 attgataaac tccgcctggc cgttgatcac gtcgatattg ggaatatctg caagattatg
     1141 aaaattct
//
LOCUS       DBFIFI010000449         1126 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.455, whole genome shotgun sequence.
ACCESSION   DBFIFI010000449 DBFIFI010000000
VERSION     DBFIFI010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.455"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..644
                     /locus_tag="N7B39_005067"
     CDS             <1..644
                     /locus_tag="N7B39_005067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system needle length
                     determinant, SpaN/EivJ family"
                     /protein_id="HGU2424833.1"
                     /translation="VDRNPSLSLQTKGAEQFSTKKLLNMTSRDQGINSELSNRTIQFK
                     EKIHNGIHTEYITDQKHSNNKDREKKYRDGDKINGPQAHSLDITNERRFADNRTMFTQ
                     HIEKQRNVNTLNQNDINNSANNANVRENELTYQFQRWGQNHTVRILESSEGIRLKPSD
                     TLVSDRLHEAQHNDVTAQRWVLTEQDERQGQRHQPHEEQENEGKFENDQKDES"
     gene            604..>1126
                     /locus_tag="N7B39_005068"
     CDS             604..>1126
                     /locus_tag="N7B39_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
                     /protein_id="HGU2424834.1"
                     /translation="MKANSKTIRRMKVNVRSEESKSKHSTFETTFQNWKENGEDVALL
                     MPEFSAKWLPIAEESGSWSGWVLLREIFPLISAELAGMALMPETERLIGEWLSLSSSP
                     LNLKYPELKYNRLCVGKVFDGVLSPAQPLIRIWTGELNLWLDKVTVCQYENAPTLDKK
                     SLYWPIHFVIGFSK"
BASE COUNT          412 a          200 c          233 g          281 t
ORIGIN      
        1 aggttgacag gaatccatct ctttctttgc aaacaaaagg agccgagcaa ttttctacaa
       61 agaaacttct caatatgact tcacgagatc agggtattaa ttctgaacta tctaatcgaa
      121 cgatacaatt taaggaaaaa atccataatg gtatccatac agaatacatc acagatcaaa
      181 aacacagtaa taataaagat cgtgaaaaaa aataccgcga tggagacaaa ataaatggac
      241 cgcaggccca ttcattagac attactaacg aaagaaggtt tgctgataac agaaccatgt
      301 ttactcaaca tatcgaaaag caaaggaatg ttaatacgct caatcagaat gatataaata
      361 attctgctaa taatgccaat gtgcgcgaaa atgaattgac atatcagttt cagcgttggg
      421 gacaaaatca tacggtccgg attctggaaa gtagtgaagg gatacgacta aaaccatcag
      481 acacacttgt ctccgacaga ctccatgaag cccaacacaa tgatgttaca gcacaacgtt
      541 gggtattgac tgagcaagat gaacgacaag gacaacgaca tcagcctcat gaggagcaag
      601 aaaatgaagg caaattcgaa aacgatcaga aggatgaaag ttaatgttcg gtctgaggaa
      661 agtaaatcga aacactctac gtttgaaacc acttttcaaa attggaaaga aaatggcgaa
      721 gatgttgcat tattaatgcc cgaatttagt gctaaatggc taccgatagc agaagagagt
      781 ggcagctggt ctggatgggt gttgcttcga gaaatatttc ccttaatttc tgccgagctc
      841 gctgggatgg ctttaatgcc agaaacggaa aggttaatag gggaatggct cagtttatcc
      901 agttccccgt taaatctaaa gtatcctgaa ctaaaatata atcgtttgtg cgtaggtaaa
      961 gtatttgatg gagtattgag tccagctcaa ccactgataa gaatatggac aggggaattg
     1021 aatctttggt tagataaagt cacagtctgc caatacgaaa acgctccaac tttagacaaa
     1081 aaatcattat attggcccat tcattttgta attggattta gtaaaa
//
LOCUS       DBFIFI010000450         1121 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.456, whole genome shotgun sequence.
ACCESSION   DBFIFI010000450 DBFIFI010000000
VERSION     DBFIFI010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.456"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..114)
                     /locus_tag="N7B39_005069"
     CDS             complement(<1..114)
                     /locus_tag="N7B39_005069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424835.1"
                     /translation="MPNKPCPACNALSGFLEKGGYYIFNCPEHVEFHISKLD"
     gene            688..876
                     /gene="dicB"
                     /locus_tag="N7B39_005070"
     CDS             688..876
                     /gene="dicB"
                     /locus_tag="N7B39_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HGU2424836.1"
                     /translation="MEKLSCNASTSELRFEIGVITGDKTFIEDAIKQRKLEQDLLNEV
                     CIPSMLARLDLLQKGYKQ"
     gene            873..1064
                     /locus_tag="N7B39_005071"
     CDS             873..1064
                     /locus_tag="N7B39_005071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HGU2424837.1"
                     /translation="MNTTFALVLTVYLVSGESLELVTGLYGSMKECMAAAAEQKIPGN
                     CYPVDKTTHTNNNEIPAGL"
BASE COUNT          320 a          241 c          261 g          299 t
ORIGIN      
        1 gtcaagtttt gatatatgaa attcaacatg ttctggacag ttaaaaatgt aataaccgcc
       61 cttctctaaa aagccagaaa gagcgttaca ggcagggcat ggtttgtttg gcattttatc
      121 ctccattaag ggctgaatta aaaatggaga ccaacacgct gctacgtgtg gtcgtgcgcc
      181 ggacacagat aagaatccgg cactgacagt ttactgaaag gatatatccc tgaaaagtca
      241 gggcataaca cgaaagtgca cggcgaagtc cttctccctt agagccgtcg ttaaatttaa
      301 ttcgaccgtg cgcttccggt tgtggcaatc cgcgaaatgg cgcggcggta agtatggcgg
      361 ggttattctt tccccgttga ggacaccggg ttgtcaggtt gaccatacgc ttaagtgaca
      421 accccgctgc aacgccctct gttatcaata ttctggtgac atttggcggt atcagtttta
      481 ctccgtaact gctctgccgc ccttttttaa aagtgaattt tgtgatgcgg tgaatgcggc
      541 tcagcgcacg cggaacagtt aaatcggtaa agcggtcatt tgctgagtaa cgagaatgct
      601 ctgtatccgg cgttaattgt taactggtta acgtcacctg gaggcaccag gcaccgcatc
      661 acaaaattca ttgttgagga cgcgataatg gaaaagttat catgtaatgc cagcacgtct
      721 gaacttcgtt tcgaaattgg cgttatcact ggagacaaaa catttattga agacgccatt
      781 aagcagagaa aactcgagca ggacctgtta aatgaagtat gcattccttc aatgctggct
      841 cgtctggacc tgctgcaaaa aggatataaa caatgaatac aacatttgca ctcgttctga
      901 cagtttatct tgtttccggc gaatctcttg agctggtgac tggcttatac ggttcaatga
      961 aagaatgcat ggctgcagca gcagaacaaa aaattcccgg taactgttat ccggtagata
     1021 aaactactca cactaataat aacgaaatac cggcaggact ttaaaacagc accgtaatta
     1081 atatccggtt tcatttttat atgccagcaa tggcagggat t
//
LOCUS       DBFIFI010000451         1101 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.457, whole genome shotgun sequence.
ACCESSION   DBFIFI010000451 DBFIFI010000000
VERSION     DBFIFI010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.457"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..>1101
                     /locus_tag="N7B39_005072"
     CDS             <1..>1101
                     /locus_tag="N7B39_005072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat domain-containing protein"
                     /protein_id="HGU2424838.1"
                     /translation="ETQREYRLDRNGQVTAVTASGTGLGYGEGDESYGYDSCGYLKAQ
                     SAGGHRISEETDQYAGGHRLKQAGNTQYDYDAAGRMVSRTRHRDGYRPETERFRWDSR
                     DQLTGYCSAQGEQWEYRHDASGRRTEKRCDRKKIRFTYLWDGDSIAEIREYRDDELYS
                     VRHLVFNGFELISQQCSRVRQPHPSVAPQWVTRTNHAVNDLTGRPLMLFNSEGKTVWR
                     PGQTSLWGLALSLPADTDYPDPRGERDAEADPGLLYAGQWQDAESGLCYNRFRYYEPE
                     TGMYLVSDPLGLQGGEQTYRYVPNPCGWVDPLGLAASSKISSLMDYIGDGRRVSGHTG
                     FLDGVRLSRSQINNIAKEMEKLGIKVIRKADK"
BASE COUNT          266 a          274 c          372 g          189 t
ORIGIN      
        1 ggaaacacag cgggagtacc ggcttgaccg gaacggccag gtcacggcgg tgacagcctc
       61 aggcaccggg ctgggctatg gcgaaggcga tgagtcctat ggctatgaca gctgtggcta
      121 cctgaaggcg cagtctgcgg gcgggcaccg gataagtgaa gagactgacc agtatgccgg
      181 aggccaccgg ctgaaacagg ccggaaacac gcagtatgac tatgacgccg caggccggat
      241 ggtcagccgc accagacacc gtgacggcta tcgcccggaa acagagcggt tccggtggga
      301 cagccgggac cagctgaccg ggtattgcag cgcacagggt gagcagtggg aataccgcca
      361 cgacgccagc ggcagacgaa cagagaaacg ctgcgaccgg aagaaaatcc gcttcacgta
      421 cctgtgggac ggcgacagta ttgcggaaat ccgggaatac cgcgatgatg aactgtacag
      481 cgtacggcac ctggtgttta acggctttga gctgataagc cagcagtgca gccgggtacg
      541 gcagccgcac ccgtccgtgg ccccgcagtg ggtgacacga acgaatcatg cggtgaacga
      601 cctgacgggc cgtccgctga tgctctttaa cagtgaaggt aaaaccgtct ggcggccggg
      661 acagaccagc ctgtgggggc tggcactcag cctgcccgca gacacagact acccggaccc
      721 gcgcggggag cgggacgcgg aagccgaccc cggcctgctg tatgcaggac agtggcagga
      781 tgcagaatcg gggttgtgct ataaccggtt ccgatattac gagccggaaa ccgggatgta
      841 cctggtgagt gatccactgg ggttgcaggg aggggagcag acttaccggt atgtgccgaa
      901 tccttgtggg tgggttgatc cgctgggatt ggctgcaagt tctaaaatca gcagtttgat
      961 ggactatatt ggcgatggtc gtcgtgttag tgggcatacg ggtttcctgg atggggttcg
     1021 tttatcacgt agtcaaataa acaatattgc taaagaaatg gagaagctag gaattaaagt
     1081 aataaggaaa gcagataaat a
//
LOCUS       DBFIFI010000452         1096 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.458, whole genome shotgun sequence.
ACCESSION   DBFIFI010000452 DBFIFI010000000
VERSION     DBFIFI010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.458"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(35..>1096)
                     /locus_tag="N7B39_005073"
     CDS             complement(35..>1096)
                     /locus_tag="N7B39_005073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HGU2424839.1"
                     /translation="LAQAFRVIGHDVVISSAGKFANKAAEAGLVVFDAVPGLDSEAGY
                     RHQEELRKKSNIIGHFSFFSDEMADNLIDFAGKWRPDLIVYPPLDPAGPLVAAKYRIP
                     SVMLAVGFAHTSAHIQMLNRSLSNAYRRHGVSGPLCDLAWIDVAPPSMSILKNAGEPV
                     ISMRYIPYNGGAVKETWWDRDSDRKRLLISLGTVKPMVDGLELISWVMDSANEVDADI
                     ILQLAINARTGLRKLPSNVRLVDWIPMGVFLNGADGFIHHGGAGNTLTALYSGIPQIV
                     FGEGADRSVNAEIVAKRGCGIIPDKHGLTSDLVNRLLYDDSLRFCSDQVAAEMAEQPS
                     PAEIAEVLMRKLKNNGKQL"
BASE COUNT          326 a          275 c          195 g          300 t
ORIGIN      
        1 cataacattt ctcgttgaaa atttcagcaa tacgctacaa ttgtttcccg ttgtttttta
       61 attttctcat caaaacctct gcaatctctg caggactggg ttgttcagcc atttcagcgg
      121 ctacctgatc tgaacagaag cgtagtgaat catcataaag aaggcgattt accaaatcac
      181 tggtcagtcc atgcttgtcc ggaataatcc cacacccacg cttcgcaaca atttctgcat
      241 taacagagcg atctgcacct tcgccaaaca caatctgtgg tattccacta tacagcgctg
      301 tcagggtatt acctgcgcca ccgtgatgaa taaatccatc agctccatta aggaatacac
      361 ccataggtat ccagtcaacc agacgtacat ttgatggtag ttttcgtaaa ccagtacgag
      421 catttattgc aagttgcaaa atgatatcag catcaacttc atttgcagaa tccataaccc
      481 atgaaatcag ctccagacca tcaaccattg gttttacagt gccaaggctg ataagtaaac
      541 gtttccgatc agaatccctg tcccaccatg tttcctttac agcacctccg ttataaggaa
      601 tatatctcat tgagataacc ggttctccag catttttaag aatgctcata cttgggggag
      661 caacatcaat ccatgctaaa tcacatagtg gaccgctgac tccatgccgc ctgtaagcat
      721 tgcttaaaga acggtttaac atctgaatat gggcagatgt atgcgcgaat ccaacagcca
      781 gcatcactga aggaattcta tatttagcag caaccaatgg gcctgccgga tcaagagggg
      841 gatagactat taaatctggc ctccattttc ctgcaaaatc gatgaggtta tctgccattt
      901 catcgctaaa aaaagagaaa tgaccaataa tattactttt tttcctcaac tcttcctgat
      961 ggcgatatcc agcctctgaa tctaaacctg gaactgcatc aaaaacaacc agtccagctt
     1021 ctgctgcttt attcgcgaat ttgccagcgc tactaattac tacatcatgt ccaattacac
     1081 gaaaggcctg agccag
//
LOCUS       DBFIFI010000453         1087 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.459, whole genome shotgun sequence.
ACCESSION   DBFIFI010000453 DBFIFI010000000
VERSION     DBFIFI010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.459"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..>1087
                     /locus_tag="N7B39_005074"
     CDS             <1..>1087
                     /locus_tag="N7B39_005074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HGU2424840.1"
                     /translation="DSRGQLIAVKDTQGHETRYEYNAAGDLTTVIAPDGSRNGTQYDA
                     WGKAICTTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRYDVLDRLIQETGFDGRTQRY
                     HHDLTGKLIRSEDEGLVTHWHYDEADRLTHRTVKGETAERWQYDERGWLTDISHISEG
                     HRVTVHYGYDEKGRLTGERQTVHHPQTEALLWQHETRHAYNAQGLANRCIPDSLPAVE
                     WLTYGSGWLAGMKLGDTPLVDFTRDRLHRKTLRRFGRYELTTAYTPAGQLQSQHLNSL
                     QYDRDYTWNDNGELIRISSPRQTRSYSYSDSGRLTGVHTTAANLDIRIPYATDPAGNR
                     LPDPELHPDSTLSMWPDNRIARDAHYLY"
BASE COUNT          259 a          352 c          318 g          158 t
ORIGIN      
        1 cgacagccgt ggacagttaa ttgccgtgaa agacacgcag ggccatgaaa cgcggtatga
       61 atacaacgcc gccggtgacc tgaccaccgt cattgccccg gacggcagca gaaacgggac
      121 acagtacgat gcgtggggaa aagccatctg taccacgcag ggcggtctga cgcgcagtat
      181 ggaatacgat gctgccggac gggtcatccg cctgaccagt gaaaacggca gccacaccac
      241 cttccgttac gatgtactcg accggctgat acaggaaacc ggctttgacg gccgcacaca
      301 gcgttatcac cacgacctga ccggcaaact tatccgcagc gaggatgagg ggctggtcac
      361 ccactggcac tatgacgaag cagaccgcct cacgcaccgc accgtgaagg gtgaaaccgc
      421 agagcgctgg cagtatgacg aacgcggctg gctgacagac atcagccata tcagcgaagg
      481 gcaccgggtg acggtgcatt acgggtatga tgagaaaggc cggctgaccg gtgagcgtca
      541 gacggtgcat cacccgcaga cggaagcact gctctggcag catgagacca gacacgctta
      601 caacgcgcag gggctggcga accgctgtat accggacagc ctgcccgccg tggaatggct
      661 gacctatggc agcggctggc tggcaggcat gaagctcggc gacacaccgc tggtggattt
      721 cacccgcgac cgcctgcacc ggaaaacgct gcgcagattc ggccgttatg aactcaccac
      781 cgcttatacc cctgccgggc agttacagag ccagcacctg aacagcctgc agtatgaccg
      841 cgattacacc tggaacgaca acggcgaact catccgcatc agcagcccgc gccagacccg
      901 gagttacagc tacagcgact ccggcaggct gaccggcgtt cacaccaccg cagcgaatct
      961 ggatatccgc atcccgtatg ccacggaccc ggcaggtaac cgcctgcccg acccggagct
     1021 gcacccggac agcaccctca gcatgtggcc ggataaccgt atcgcccgtg acgcgcacta
     1081 tctttac
//
LOCUS       DBFIFI010000454         1084 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.460, whole genome shotgun sequence.
ACCESSION   DBFIFI010000454 DBFIFI010000000
VERSION     DBFIFI010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.460"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..626)
                     /gene="nleH1-1"
                     /locus_tag="N7B39_005075"
     CDS             complement(<1..626)
                     /gene="nleH1-1"
                     /locus_tag="N7B39_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector kinase
                     NleH1-1"
                     /protein_id="HGU2424841.1"
                     /translation="MLSPYSVNLGCSWNSLTRNLTSPDNRVLSSVRDAAVHSDNGAQV
                     KVGNRTYRVVATDNKFCVTRESHSGCFTNLLHRLGWPKGEISRKIEVMLNASPVSAAM
                     ERGIVHSNRPDLPPVDYAPPELPSVDYNRLSVPGNVIGKGGNAVVYEDAEDATKVLKM
                     FTTSQSNEEVTSEVRCFNQYYGAGSAEKIYGNNGDIIGIRMDKINGESL"
     gene            complement(796..951)
                     /locus_tag="N7B39_005076"
                     /pseudo
     CDS             complement(796..951)
                     /locus_tag="N7B39_005076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
BASE COUNT          338 a          265 c          161 g          320 t
ORIGIN      
        1 agcgattctc catttatttt atccattcta ataccaataa tatcaccatt attgccatat
       61 attttttctg cactcccggc accataatat tggttgaagc aacgaacttc gcttgtcacc
      121 tcttcattgc tttgagatgt agtaaacatc ttcaggactt ttgttgcatc ctcagcatct
      181 tcatatacta cagcgttccc ccctttgcca ataacattac caggtactga caacctgtta
      241 tagtccacac tcggtaactc tggcggtgca taatcaacag gaggtaaatc aggtctgttc
      301 gaatgaacaa tgcctctttc catagcagcg ctcactggtg atgcattcag catgacctca
      361 attttcctgc taatctcccc cttaggccat cccagcctgt gcaacagatt agtaaaacaa
      421 ccactatgac tttctcttgt aacgcaaaac ttattatcgg tggcaacaac acgatatgtt
      481 ctgttgccaa cctttacttg cgccccatta tcagaatgaa cggcagcatc ccttacagag
      541 gataaaacac gattatcagg cgaagtcagg tttctggtta aagaattcca tgaacatccc
      601 aaatttacag aatatggcga taacatacat ttcaaccttc aaaataaacc catctaaatt
      661 tccttaacaa acatcccccc taacatggca acaaaaatta gaaatgaact cttgcttttt
      721 gtatctggca actaattcat ccttccagca tcccatcaaa cgaacgttta cgaactataa
      781 cgcaggtatt accggtcact cgatatctgg agatacagac gtatcaacat gattgagcgg
      841 catacgatac tgcttccatg ccgccagtaa cgatgtctgc tcatccgtcg caatatctaa
      901 atctgcagca tcctgaagtg gtgcgatata ctcactgatt acaagtacca gaccgataga
      961 ttaaaaaaaa cacaataaca cgctgcattt aaaacaaaaa attttaaata tgatttaata
     1021 caagctttaa gcctttaatt atcagtttta tttattttcc agcaattgat taatcaattg
     1081 ttta
//
LOCUS       DBFIFI010000455         1076 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.461, whole genome shotgun sequence.
ACCESSION   DBFIFI010000455 DBFIFI010000000
VERSION     DBFIFI010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.461"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..532)
                     /locus_tag="N7B39_005077"
     CDS             complement(<1..532)
                     /locus_tag="N7B39_005077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HGU2424842.1"
                     /translation="MAAVQISGVLKDGAGKPIQNCTIQLKARRTSSTVVVNTVASENP
                     DEAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMSEDDVRPE
                     ALRRFELMVEEAARHAEEAKKNAGEAETSARNAGISASQAEESAANADTSAGDASESA
                     RQAAESAAAAKQSEEAS"
     gene            complement(592..>1076)
                     /locus_tag="N7B39_005078"
     CDS             complement(592..>1076)
                     /locus_tag="N7B39_005078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HGU2424843.1"
                     /translation="TNVPGSDDLNGINVKYRYEFTDTLGMVTSFSYAGDKNRQITRYS
                     DTRWHEDSVRNRWFSVMAGPSVRVNEWFSAYAMAGVAYSRVSTFSGDYLCVTDSKGKR
                     HDVLTGSDDGRHSNTSLAWGAGVQFNPTESVAIDVAYEGSGSGDWRTDGFIVGVGYKF
                     "
BASE COUNT          218 a          354 c          250 g          254 t
ORIGIN      
        1 aggacgcctc ctctgactgc tttgcagcgg ctgcactttc tgccgcctgc cgggctgact
       61 ccgatgcatc ccctgctgaa gtgtcagcat ttgcagcgct ctcttctgcc tgactggctg
      121 atatgccggc attcctcgct gacgtctccg cctctccggc attcttcttc gcctcctcag
      181 cgtgacgcgc cgcttcttcc accatcagtt caaaacgacg cagtgcctcc ggccggacgt
      241 catcctccga catggcaccg agaaaatcat tcagcgtccc cggttgagaa tcttcataca
      301 cggtgatggt cccggcatgg gatggcggga atccttccac caacagaata acgctgtact
      361 gcccgtactc aacgtccatg ctgtaacgac cggcttcatc cggattttca gaggccaccg
      421 tgttcaccac caccgtgctg ctggtccgtc tggctttcag ttgaatggtg cagttctgta
      481 ttggttttcc cgcaccatct ttcagcacgc ctgatatttg tactgctgcc ataccctctc
      541 cacaaaaaag cccgcctgaa ccggcgggct gtcataacgc tgtgttacta atcagaattt
      601 ataaccgaca cccacgatga aaccgtcagt gcgccagtca ccactgccgg aaccttcata
      661 agcgacatca atggccacgg attcggtcgg gttaaactgc acgccagctc cccacgccag
      721 agacgtgttg ctgtggcgac cgtcatcact tccggtcagc acgtcgtgcc ttttcccctt
      781 gctgtcagtt acgcagagat aatccccgga aaaagtcgac acacggctgt aagccacacc
      841 cgccatcgca tacgcgctga accattcatt cacgcgtaca gacggccccg ccatcacgct
      901 gaaccagcgg ttacgcacgg aatcttcatg ccagcgggta tcgctgtaac gggtaatctg
      961 gcgattcttg tctcctgcat agctgaatga cgtcaccatc cccagtgtgt ccgtaaactc
     1021 ataacggtat ttcacgttaa tcccgttcag atcatcgctg ccgggaacgt tcgtcg
//
LOCUS       DBFIFI010000456         1073 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.462, whole genome shotgun sequence.
ACCESSION   DBFIFI010000456 DBFIFI010000000
VERSION     DBFIFI010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.462"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..527
                     /gene="espM2"
                     /locus_tag="N7B39_005079"
     CDS             <1..527
                     /gene="espM2"
                     /locus_tag="N7B39_005079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector guanine nucleotide exchange factor
                     EspM2"
                     /protein_id="HGU2424844.1"
                     /translation="FRNSFRGKNDEVVKCSIGERTISFSVRKFSGNILETVRRQSTKD
                     IDEWIKDERIVYPSRVINQEIDNYCFQKNAKISTEERQRVFFLVSQENQLTLDVKAAQ
                     SSINHVIMGSASFGKKMDALCDGMSRDVKNRTSDTIANLLADKFYQKHIDSDIDIVKL
                     RNDIPDYLMRAIQG"
     gene            761..913
                     /locus_tag="N7B39_005080"
                     /pseudo
     CDS             761..913
                     /locus_tag="N7B39_005080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885624.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
BASE COUNT          344 a          198 c          255 g          276 t
ORIGIN      
        1 ccttcaggaa ctctttccga gggaagaatg atgaggtcgt aaaatgttca attggagaac
       61 ggactattag cttttctgtt cgcaaattta gcggcaatat actggaaacg gtgagaaggc
      121 agagtactaa agatattgat gagtggataa aagatgaacg gatagtatat ccctcaaggg
      181 tgatcaacca ggagattgat aattactgtt ttcaaaaaaa tgcaaaaatt tccactgaag
      241 agaggcaaag agtctttttt cttgtgagcc aggagaatca actaaccctt gatgttaagg
      301 cagcacaaag ttctattaat catgtaataa tggggagtgc ttcttttggc aagaaaatgg
      361 atgctctttg tgatggtatg agccgggatg taaaaaatcg tacatcagat accatagcaa
      421 acttgcttgc ggacaagttt taccagaaac atatcgattc ggatattgat attgtaaaac
      481 tacgaaatga tatcccagat tatttgatgc gtgctataca gggatgaggc tagaggagag
      541 gactgcttca gcatcatcgg cttcggacca gtaaaagaga cgcagaaaat gcacaacggc
      601 accacacgcc atgcatggat tcagattact catggctcac cagtacagct ataaatcgta
      661 aagaaaacta cagtacgtta tacacagaaa cgtaccgtga ttcattattt attgtacagg
      721 ttcatcgcag cgacaatatt ttgtgatgtt tgcgtggtgc gccccacttc aggatgctgc
      781 agatctggaa attgcaacgg aggaagaaac ctcattgctg gaagcctaga aaaagtatcg
      841 ggtgttgctg aaccgtgttg atacatcaac tgcacctgat attgagtggc cggaagaacc
      901 agacacaatg taagcgaaaa agaaaaaccg cagacacgac gtatgcagga cgtgctgcgg
      961 ttggctggtg aactttcgat agtgcgagta ttgaatgatt tccagccgtt accgatttta
     1021 cgtgttaatt agtgaacaaa ccactcgtca gcagacttcc aggtatcttt cag
//
LOCUS       DBFIFI010000457         1064 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.463, whole genome shotgun sequence.
ACCESSION   DBFIFI010000457 DBFIFI010000000
VERSION     DBFIFI010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.463"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            78..775
                     /locus_tag="N7B39_005081"
     CDS             join(78..327,327..775)
                     /locus_tag="N7B39_005081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1F family transposase"
                     /protein_id="HGU2424845.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSPCRKTWQLQFT
                     YTASQPGKHQKIIDMAMNGVGCRASARIMGVGLNTVLRHFKKLRPQSVTSRIQPGSDV
                     IVCAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEV
                     VVWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELH
                     DKVIGHYLNIKHYQ"
     gene            792..1052
                     /locus_tag="N7B39_005082"
                     /pseudo
     CDS             792..1052
                     /locus_tag="N7B39_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390635.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
BASE COUNT          266 a          244 c          273 g          281 t
ORIGIN      
        1 aatgtttacc ttatcatttt ctggtaatgc tgccaactta ctgatttagt gtatgatggt
       61 gattttaagg tgcttgcgtg gcttccattt ccatcagatg tccttcctgc tccgctactg
      121 aaggcgtggt gcgtaacggc aaaagcactg ccggacatca gcgctatctc tgctctcctt
      181 gccgtaaaac atggcaacta cagttcactt acaccgcctc tcagcccggt aagcaccaga
      241 aaatcattga tatggccatg aatggcgtcg gatgtcgcgc cagtgcacgc attatgggcg
      301 ttggcctcaa cacggtttta cgtcacttaa aaaactcagg ccgcagtcgg taacctcgcg
      361 catacaaccg ggcagtgatg tgattgtctg cgctgaaatg gacgaacagt ggggctacgt
      421 cggtgctaaa tcacgtcagc gctggctgtt ttacgcgtat gacaggatac ggaggacggt
      481 tgtggcgcac gtcttcggtg aacgcactct ggccacactg gagcgtcttc tgagcctgct
      541 gtcggccttt gaggtcgtgg tatggatgac ggatggctgg ccgctgtatg aatcacgcct
      601 gaagggaaag ctgcacgtta tcagcaagcg ttacactcag cgcattgagc gacataatct
      661 gaatctgaga caacatctgg caaggctggg acggaagtca ctgtcgttct caaaatcggt
      721 ggagctgcat gacaaggtca tcgggcatta tctgaacata aaacactatc agtaagttgg
      781 agtcattacc tgagaataat acctcctggg taagtgagca gatactttca gcgcctccga
      841 aagatgtaaa tattcgactt ggagtcggtt ctttagaagg tacaaccgtc tcacatgttc
      901 agcgattgca tcattcgtta atcgcagctg gtttggaaag taacctcact gtctataccg
      961 gtggtcacga ttatgcctgg tggcgcggag caattattga tgaattagca aattataatt
     1021 gcaggaaggt atcaaataat aactctgtgt aaaatatcgt ttta
//
LOCUS       DBFIFI010000458         1063 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.464, whole genome shotgun sequence.
ACCESSION   DBFIFI010000458 DBFIFI010000000
VERSION     DBFIFI010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.464"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            151..258
                     /gene="yniD"
                     /locus_tag="N7B39_005083"
     CDS             151..258
                     /gene="yniD"
                     /locus_tag="N7B39_005083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HGU2424846.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(311..>1063)
                     /locus_tag="N7B39_005084"
     CDS             complement(311..>1063)
                     /locus_tag="N7B39_005084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HGU2424847.1"
                     /translation="LLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFEGN
                     LNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGGRS
                     RFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRYDK
                     YTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHFGL
                     KVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
BASE COUNT          288 a          261 c          253 g          261 t
ORIGIN      
        1 tcgatacata attttatttt ttcataaaac gcagaaacag tgatcaggtg tacaacgcca
       61 cccgacggct gtaataacaa aaaatccctt gatgcctgtc ccttttgtta cactccgtta
      121 tcacgcacaa gagatatgca ggacactggt atgccgacta aacgctttga taaaaaacac
      181 tggaagatgg tggtggtgct actggcaatc tgtggcgcta tgttgttgct acgttgggca
      241 gcaatgattt ggggctgagc agtgacaaac gaagacagcc agaccgcata acacggtccg
      301 gcagataatt ttacatgctg tagcccagcg ataaggttgt ccgacggtcg gtatgttccg
      361 gtgcagactc tggaggttca gagttccatg tgacgttgta agccaccttc agtccaaaat
      421 gctcattaat agcaacattt aatgcgcttt cggagttcag cgttgtgtct tccgcgccaa
      481 agacggaaac accctgcgta aatttagcgt tgtcagtaag ctgccatgca taagcaccgg
      541 aggcatagcc cagcggctga gtttcactgg cattgtcggt atatttgtcg taacgcacac
      601 ctggaccgaa ttcaaagcgg aaactgtgta ccgggccatt caaaaactga cgaccataac
      661 ccgcggtcaa cacatcgcgc tctcgatagc cgttataacg gtctgtcagc cagcttgcct
      721 gaccaaataa atagtcataa tcagttaaat taaaacggct acgcccgccc gccgcatatt
      781 tttctgaaga acgctcatca ttagaagaag tattactggc gttcccccac agcgaccagg
      841 cagtggtttg tccataccag gtcatggtgg tgtcagccgt aagtgaggag cttttcgtat
      901 tgcctgattg tgcaagatac cctgcgttca gattaccttc gaaaggtttt ttagcgctgg
      961 caggatcgtc catgacagta aaaacggaat catcggcagc tgcattcagt gacgcaaaca
     1021 tgccccccgc caacataacg atggcaggaa ctgtcttcaa aag
//
LOCUS       DBFIFI010000459         1053 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.465, whole genome shotgun sequence.
ACCESSION   DBFIFI010000459 DBFIFI010000000
VERSION     DBFIFI010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.465"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..308
                     /gene="escQ"
                     /locus_tag="N7B39_005085"
     CDS             <1..308
                     /gene="escQ"
                     /locus_tag="N7B39_005085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE ring protein EscQ"
                     /protein_id="HGU2424848.1"
                     /translation="NTPLATVNLSDNNLSVTDVLAADLLSALSNETVMNRIYCAIGTV
                     HIDIHMLRNVKKDDIINSDGYHLFGGCQLIRNNTTIAYGSIVKINEDFYFTVSIVCD"
     gene            339..845
                     /gene="espH"
                     /locus_tag="N7B39_005086"
     CDS             339..845
                     /gene="espH"
                     /locus_tag="N7B39_005086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector EspH"
                     /protein_id="HGU2424849.1"
                     /translation="MSLSGAVFKTFLTSEHASWNRFNRRLHIPNEDIVDEIQLKARMQ
                     QRHHRVYPEIGDSTIVSFRGKDYAVHFIKDGPKDDYVYKVQRITPENGCFSTLFSVFS
                     GGVTKALERKLNERHITPLSSTWFPRTPLEGILAERGLSSLLRRVQSTERLDNRAIAT
                     RASSYSVL"
BASE COUNT          325 a          183 c          218 g          327 t
ORIGIN      
        1 ttaatacccc tttagcaaca gtaaatctga gtgataataa tttatctgtc acggatgttc
       61 tggctgcgga tctgttatct gctcttagca atgagactgt catgaatcgc atttattgtg
      121 cgatcggtac agtccatatt gatatacaca tgttgcgtaa tgttaaaaaa gatgacatta
      181 ttaattcaga tggataccat ctttttggtg gttgtcagtt aatacgcaat aatacgacga
      241 tagcatacgg gagtattgta aaaatcaacg aagattttta ttttactgtt agcatagtat
      301 gtgattaatc aggaatacgt tatataggga gatgtatcat gtcgttatca ggagcggtat
      361 tcaagacatt tctgacaagt gaacatgcgt catggaacag gttcaacaga aggcttcata
      421 tacccaatga agatatcgta gatgaaattc agcttaaagc ccgaatgcaa caacgccatc
      481 atcgggtata tccagaaata ggggattcca ccattgtctc ttttagaggc aaagactatg
      541 ctgttcactt tattaaagat ggcccaaaag atgattatgt gtacaaagta caaagaataa
      601 caccggaaaa tggatgtttc tcaacgctat ttagcgtgtt ttctggcggt gtgactaagg
      661 ctttggaaag aaaattaaat gagagacata taactccact ttcttcaacc tggtttcctc
      721 gtactcccct ggaggggatc ttagctgaaa gaggattatc ttctttgtta cgtcgtgtac
      781 agtctaccga acgtcttgat aatagggcta tagcaacaag agcttcctct tatagcgtat
      841 tatgattagc atttgagttt tcttcctgac ttaactccag aatagttata attctggagt
      901 ttttccgctt tttaactgat attacattgc ttttagataa tacaggctga atcttcagag
      961 acgttagcgg ccagaaatca gaggtccctg tgatgaagag tatgttaaac ggaatggatg
     1021 atcccatcca gcgcagaaat tatcaaggta aaa
//
LOCUS       DBFIFI010000460         1033 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.466, whole genome shotgun sequence.
ACCESSION   DBFIFI010000460 DBFIFI010000000
VERSION     DBFIFI010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.466"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(137..>1033)
                     /locus_tag="N7B39_005087"
     rRNA            complement(137..>1033)
                     /locus_tag="N7B39_005087"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          229 a          326 c          231 g          247 t
ORIGIN      
        1 cggcatgggg tcaggtggga ccaccgcgct aaggccgcca ggcaaattct gttttatcag
       61 accgcttctg cgttctgatt taatctgtat caggccgaaa atcttctctc atccgccaaa
      121 acaccttcgg cgttgtaagg ttaagcctca cggttcatta gtaccggtta gctcaacgca
      181 tcgctgcgct tacacacccg gcctatcaac gtcgtcgtct tcaacgttcc ttcaggaccc
      241 ttaaagggtc agggagaact catctcgggg caagtttcgt gcttagatgc tttcagcact
      301 tatctcttcc gcatttagct accgggcagt gccattggca tgacaacccg aacaccagtg
      361 atgcgtccac tccggtcctc tcgtactagg agcagccccc ctcagttctc cagcgcccac
      421 ggcagatagg gaccgaactg tctcacgacg ttctaaaccc agctcgcgta ccactttaaa
      481 tggcgaacag ccataccctt gggacctact tcagccccag gatgtgatga gccgacatcg
      541 aggtgccaaa caccgccgtc gatatgaact cttgggcggt atcagcctgt tatccccgga
      601 gtacctttta tccgttgagc gatggccctt ccattcagaa ccaccggatc actatgacct
      661 gctttcgcac ctgctcgcgc cgtcacgctc gcagtcaagc tggcttatgc cattgcacta
      721 acctcctgat gtccgaccag gattagccaa ccttcgtgct cctccgttac tctttaggag
      781 gagaccgccc cagtcaaact acccaccaga cactgtccgc aacccggatc acgggtccac
      841 gttagaacat caaacattaa agggtggtat ttcaaggtcg gctccatgca gactggcgtc
      901 cacacttcaa agcctcccac ctatcctaca catcaaggct caatgttcag tgtcaagcta
      961 tagtaaaggt tcacggggtc tttccgtctt gccgcgggta cactgcatct tcacagcgag
     1021 ttcaatttca ctg
//
LOCUS       DBFIFI010000461         1020 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.467, whole genome shotgun sequence.
ACCESSION   DBFIFI010000461 DBFIFI010000000
VERSION     DBFIFI010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.467"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..265)
                     /locus_tag="N7B39_005088"
     CDS             complement(<1..265)
                     /locus_tag="N7B39_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311760.1"
                     /note="may be involved in the export or assemby of
                     proteins involved in the entry of Salmonella into host
                     cells; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscC/YscC/HrcC family type III secretion system
                     outer membrane ring protein"
                     /protein_id="HGU2424850.1"
                     /translation="MKIKLRITIILISVLCIFNGLLTPGAYAAAANGYVANKENLRSF
                     FETVSSYAGKPTIVSKLAMKKQISGNFDLTEPYALIERLSAQMG"
     gene            complement(262..1011)
                     /locus_tag="N7B39_005089"
     CDS             complement(262..1011)
                     /locus_tag="N7B39_005089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU2424851.1"
                     /translation="MIEEGLLLPMNVLLQGQKLTLLDPEMWFLRGNENKDVTLVITIG
                     NNHQKLMVVEDILLLIDRSQIEVTAGKVIYHPLRIDILSKLLAFIDESTGSVEREHHE
                     LFADALPFASEVVLFSKVASEAWFLATYLSSDNINEILFQHLRKTECYKLVRYLLSQS
                     LIQTSLYDLGELYGVSYSHFRRLCSYALGGKVKTELCGWRVARAVLEIIEGNSDMTTI
                     AHKYGYSSSSHFSAEVKSRLGKTPRELCKKL"
BASE COUNT          304 a          220 c          171 g          325 t
ORIGIN      
        1 gccccatctg tgccgacagg cgttcaatca aggcataggg ttctgttaaa tcaaaatttc
       61 cgctgatctg tttcttcatg gcaagcttac taactattgt aggcttaccc gcatatgatg
      121 aaaccgtttc aaaaaaacta cgtagatttt ctttatttgc tacgtatcca ttcgctgctg
      181 cggcatatgc accaggagtc aataatccat taaaaatgca taagactgaa attaatataa
      241 tagtaatgcg taattttatt ttcataactt tttacatagt tctctcggtg tttttcctaa
      301 ccggcttttt acttcggctg aaaagtgtga tgaggacgag taaccatatt tatgcgcaat
      361 agttgtcata tcactgtttc cttcaatgat ttccaacacg gctctggcaa ctctccagcc
      421 acataattcc gttttaactt taccacccaa tgcataacta cataaacggc gaaaatgtga
      481 atacgatacg ccgtaaagct caccaagatc atagagtgaa gtctgtatga gcgattgcga
      541 taataaataa cgaaccagtt tatagcattc tgtttttctt agatgctgaa acaggatttc
      601 attaatatta tcgctgctca gataagtggc taaaaaccag gcttcacttg caactttact
      661 gaatagcaca acttctgatg caaagggcaa ggcatcagca aacaattcat ggtgttccct
      721 ttccacactc cccgttgatt catctataaa agctaataat ttactcagga tatcaatgcg
      781 aagtggatga taaattactt tccctgcagt tacctcaatt tggcttcggt caatgagcag
      841 taatatatct tccactacca ttaatttttg atggttatta ccaatcgtaa taacaagggt
      901 tacatccttg ttttcatttc cccgtaagaa ccacatttca gggtccagta gggttaactt
      961 ctgtccttga agcaaaacat tcattggaag taacagccct tcttcaatca tgtttcatct
//
LOCUS       DBFIFI010000462         1011 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.468, whole genome shotgun sequence.
ACCESSION   DBFIFI010000462 DBFIFI010000000
VERSION     DBFIFI010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.468"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..774
                     /gene="yeeO"
                     /locus_tag="N7B39_005090"
     CDS             <1..774
                     /gene="yeeO"
                     /locus_tag="N7B39_005090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HGU2424852.1"
                     /translation="VAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIGIPASVE
                     SVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITGRRLGVG
                     QIAQAEIQLRHVFWLTTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILIWLNALF
                     MPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVGVWMGMF
                     ADWAVRAVLFYWRMVTGRWLWKYPRPEPQKCEKKPAVSE"
     gene            875..950
                     /locus_tag="N7B39_005091"
     tRNA            875..950
                     /locus_tag="N7B39_005091"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:908..910,aa:Asn,seq:gtt)
BASE COUNT          218 a          195 c          283 g          315 t
ORIGIN      
        1 gttgcaattt tgtgggtgct ggcgattggt tttaatcctg cgctaaggat ttcgttaaag
       61 agctatttta aaccgctgaa ttttagcatt atctgggaag tcatggggat tggtattcca
      121 gcgagtgtcg aatcagtgtt atttaccagt ggtcggttat taacccaaat gttcgttgcc
      181 ggaatgggga ccagtgttat tgccggaaat tttatcgcgt tttcaattgc ggctcttatc
      241 aacttacccg gaagtgcgct cggttctgct tctacgatca ttacaggccg aaggttgggg
      301 gtagggcaga tagcgcaagc agagattcag ttgcggcatg tgttttggct gaccactctt
      361 ggattaacgg ccatcgcctg gctaacggct ccctttgccg gggttatggc atcgttttac
      421 acccaggatc cacaggttaa acatgtcgtt gtgattctga tttggctaaa tgctttattt
      481 atgcctattt ggtccgcttc atgggtgcta cccgctggat ttaagggggc tcgtgacgcc
      541 cgttacgcta tgtgggtttc tatgttgagc atgtggggtt gtcgggttgt cgtcggttat
      601 gtgctgggca tcatgcttgg ctggggtgtg gttggtgtct ggatgggaat gtttgccgac
      661 tgggctgtgc gggccgtgct gttttactgg cgaatggtta ctggacgttg gctgtggaaa
      721 taccctcgac ccgaaccgca aaagtgtgaa aaaaagccag ctgtgtcgga ataaacgaca
      781 aaatgcagat tatttcagca aacgatttca aatttaaaaa acaggctttg acattgtggg
      841 tgggcatcgc taatattcgc ctcgttctca cgattcctct gtagttcagt cggtagaacg
      901 gcggactgtt aatccgtatg tcactggttc gagtccagtc agaggagcca aattttagtt
      961 ttgaggcatc tttatgagtc tcagggcagt taaaaaataa aaattatttt t
//
LOCUS       DBFIFI010000463         1007 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.469, whole genome shotgun sequence.
ACCESSION   DBFIFI010000463 DBFIFI010000000
VERSION     DBFIFI010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.469"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..461
                     /locus_tag="N7B39_005092"
     CDS             <1..461
                     /locus_tag="N7B39_005092"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HGU2424853.1"
                     /translation="TVQSASAKKTPYCVMIMDIDHFKKVNDTWGHPVGDQVIKTVVNI
                     IGKSIRPDDLLARVGGEEFGVLLTDIDTERAKALAERIRENVERLTGDNPEYAIPQKV
                     TISIGAVVTQGNALNPNEIYRLADNALYEAKETGRNKVVVRDVVNFCESP"
     gene            559..>1007
                     /locus_tag="N7B39_005093"
     CDS             559..>1007
                     /locus_tag="N7B39_005093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CSS-motif domain-containing protein"
                     /protein_id="HGU2424854.1"
                     /translation="MLIIDQSAITRFYWQLYYNFLGCITLMRGETCMVFNKKMFVLII
                     IPGILGVLLSFAMSVFQMNRDTTITAGILLKQLDNVTQIVKHTTKLTSILVMKPCKDI
                     LEQLIANGALTPYVRTTGLIENNFQICSSVSGFKKMNVNDVYGTSFH"
BASE COUNT          307 a          187 c          231 g          282 t
ORIGIN      
        1 tgacagttca atcagcatca gccaaaaaaa cgccttattg cgtcatgatt atggatatcg
       61 accacttcaa aaaagtcaac gacacctggg ggcatccggt tggcgatcag gtgataaaaa
      121 cagtggtgaa tatcattggg aaaagcatac gaccagatga ccttttagcg cgcgtcggcg
      181 gcgaagagtt tggcgtcttg ctgacggaca tcgatactga acgtgcgaaa gctttagcgg
      241 aaaggattcg ggaaaatgtt gagcgtttaa ctggcgataa tcctgaatac gctataccac
      301 aaaaagtgac gattagtatt ggcgctgttg tgactcaggg aaatgcgtta aacccgaatg
      361 agatctatcg actggctgat aacgcactat acgaggcgaa agagaccggg cgtaataagg
      421 tggttgtgag ggatgtggtg aatttttgtg agtcaccata aagcggcatt ttagcgggtg
      481 attttggtaa gcgctaaata gctattcgca gacatgcgta acaattttca gaaatatcat
      541 agtactatat gatcttaaat gcttatcatt gatcagtctg ctatcacccg cttttactgg
      601 caattgtact ataatttctt aggttgcatc accctgatgc gtggggaaac atgtatggta
      661 ttcaacaaaa aaatgtttgt actcattata ataccaggca ttctgggggt gttactgtcg
      721 tttgctatgt cagtatttca aatgaatcga gatacgacaa ttaccgccgg tattctgtta
      781 aaacagcttg ataatgttac gcaaattgtc aaacatacga caaagcttac ttctattctg
      841 gtgatgaaac cctgcaaaga tatcctggaa caattgattg ctaacggagc attgaccccc
      901 tatgttcgaa caacgggtct tatagaaaat aatttccaaa tatgttcttc agttagcggt
      961 ttcaaaaaaa tgaatgttaa cgatgtttac ggtacgagct ttcacaa
//
LOCUS       DBFIFI010000464         1004 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.470, whole genome shotgun sequence.
ACCESSION   DBFIFI010000464 DBFIFI010000000
VERSION     DBFIFI010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.470"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..>1004
                     /locus_tag="N7B39_005094"
     CDS             <1..>1004
                     /locus_tag="N7B39_005094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HGU2424855.1"
                     /translation="SLPAAPLVRYTYTEAGELLAVYDRSNTQVRAFTYDAQHPGRMVG
                     HRYAGRPEMRYRYDDAGRVVEQLNPAGLSYHYQYEQDRITVTDSLNRREVLHTEGGAG
                     LKRVVKKELADGSVTHSGYDAAGRLTAQTDAAGRRTEYGLNVVSGDITDITTPDGRET
                     KFYYNDGNQLTAVVSPDGLESRRAYDEPGRLVSETSRSGETVRYRYDDAYSELPATTT
                     DATGSTRQMTWSRYGQLLAFTDCSGYQTRYEYDRFGQMTAVHREEGISLYRRYDNRGR
                     LISVKDAQGHETRYEYNAAGDLTAVITPDGNRSETQYDAWGKAVSTTQGGLTRSMEYD
                     L"
BASE COUNT          229 a          281 c          353 g          141 t
ORIGIN      
        1 agcctgcccg ctgcgccact ggtgcggtac acgtatacgg aagccggtga actgctggcg
       61 gtatatgacc gcagcaatac gcaggtgcgc gctttcacgt atgacgcgca gcacccgggc
      121 cggatggtgg ggcaccgtta tgcgggaagg ccggagatgc gctaccgcta cgacgatgcg
      181 gggcgggtgg tggagcaact gaacccggca ggcctgagtt accactacca gtatgagcag
      241 gaccgcatca ccgtcacgga cagcctgaac cggcgtgagg tgctgcatac agaaggcggg
      301 gccgggctga agcgggtggt gaaaaaagaa ctggcggacg gcagcgtcac gcacagcggg
      361 tatgacgcgg caggaaggct aacggcgcag acggacgcgg cgggacggcg gacagagtat
      421 ggtctgaatg tggtatccgg cgatatcacg gacatcacca caccggacgg gcgggagacg
      481 aaattttact ataacgacgg gaaccagctg acggcggtgg tgtccccgga cgggctggag
      541 agccgccggg catatgatga accgggcagg ctggtatcgg agacatcgcg cagcggggag
      601 acagtacgct accgctacga tgatgcgtac agtgagttac cggcgacgac aacagatgcg
      661 acgggcagca cccggcagat gacctggagc cgctacggtc agttgctggc gttcaccgac
      721 tgctcgggct accagacccg ctatgaatac gaccgcttcg gccagatgac ggcggtccac
      781 cgcgaggaag gcatcagcct ttaccgccgc tatgacaacc gtggccggtt aatctcggtg
      841 aaagacgcac agggccatga aacgcggtat gagtacaacg ccgcaggcga cctgactgcc
      901 gttatcaccc cggacggcaa ccggagcgag acacagtacg atgcgtgggg aaaggcggtc
      961 agcaccacgc agggcgggct gacgcgcagt atggaatacg acct
//
LOCUS       DBFIFI010000465         1004 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.471, whole genome shotgun sequence.
ACCESSION   DBFIFI010000465 DBFIFI010000000
VERSION     DBFIFI010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.471"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..>1004
                     /locus_tag="N7B39_005095"
     CDS             <1..>1004
                     /locus_tag="N7B39_005095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HGU2424856.1"
                     /translation="SLPGAPLARYTYTEAGELLAVYDRSNTQVRAFTYDAQHPGRMVA
                     HRYAGRPEMRYRYDDTGRVVEQLNPAGLSYRYQYEQDRITVTDSLNRREVLHTEGGAG
                     LKRVVKKELADGSVTHSGYDAAGRLTAQTDAAGRRTEYGLNVVSGDITDITTPDGRET
                     KFYYNDGNQLTAVVSPDGLESRRAYDEPGRLVSETSRCGDVIRYAYDNPHSELPATTT
                     DATGSTRQMTWSRYGQLLAFTDCSGYQTRYEYDRFGQMTAVHREEGISRYRRYDNRGR
                     LTSVKDAQGHETRYEYNAAGDLTAVITPDGNRSETQYDAWGKAVSTTQGGLTRSMEYD
                     L"
BASE COUNT          225 a          279 c          352 g          148 t
ORIGIN      
        1 agcctgcccg gtgcgccact ggcgcggtac acgtatacgg aagccggtga actgctggcg
       61 gtatatgacc gcagcaatac gcaggtgcgc gctttcacgt atgacgcgca gcatccgggc
      121 cggatggtgg cgcaccgtta cgcgggaagg ccggagatgc gctaccgcta cgacgatacc
      181 gggcgggtgg tggagcagct gaacccggca ggcctgagtt accgctacca gtatgagcag
      241 gaccgcatca ccgtcacgga cagcctgaac cggcgtgagg tgctgcatac agaaggcggg
      301 gccgggctga agcgggtggt gaaaaaagaa ctggcggacg gcagcgtcac gcacagcggg
      361 tatgacgcgg caggaaggct cacggcgcag acggacgcgg cgggacggcg gacagagtac
      421 ggtctgaatg tggtgtccgg cgatatcacg gacatcacca caccggacgg gcgggagacg
      481 aaattttact ataacgacgg gaaccagctg acggcggtgg tgtccccgga cgggctggag
      541 agccgccggg catatgatga accgggcagg ctggtatcgg agacatcgcg ctgtggggac
      601 gtcatccggt atgcttatga taatccgcac agtgaattac cggccacgac aacagatgcg
      661 acgggcagca cccggcagat gacctggagc cgctacgggc agttgctggc gttcaccgac
      721 tgctcgggct accagacccg ttatgaatac gaccgctttg gtcagatgac ggcggtccac
      781 cgtgaggaag gtatcagccg ttaccgccgc tatgacaacc gtggccggtt aacctcggtg
      841 aaagacgcac agggccatga aacgcggtat gagtacaacg ccgcaggcga cctgactgcc
      901 gttatcactc cggacggcaa ccggagcgag acacagtacg atgcgtgggg aaaagcggtc
      961 agcaccacgc agggcgggct gacgcgcagt atggagtatg acct
//
LOCUS       DBFIFI010000466          996 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.472, whole genome shotgun sequence.
ACCESSION   DBFIFI010000466 DBFIFI010000000
VERSION     DBFIFI010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..996
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.472"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(90..944)
                     /gene="rclR"
                     /locus_tag="N7B39_005096"
     CDS             complement(90..944)
                     /gene="rclR"
                     /locus_tag="N7B39_005096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HGU2424857.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWSVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
BASE COUNT          268 a          263 c          228 g          237 t
ORIGIN      
        1 cccaaaccac cgttggaaat agaaatagtt gaaaggctca caactcccgg aatgcggatt
       61 atctttgccc tgatttctgg cggttttatt cagggtgcaa gctgtctgac cctttcccga
      121 tattctcccg gagtacaacc aaactcgcgg acaaacgcct tgtgaaaaga tgattcactg
      181 gcatagccta ctgactcagc gatcaccaca acagggagca tttcccggga aaacatctgg
      241 gccgctattt gtagacgcaa ctttgttaat acagccagcg gcgtggttcc ggaaacatca
      301 cggaaaagct gggcaaaact tgcccgggac atgtgggcga tgctggccag cgattcgacg
      361 ctccaggcgt gtcctggcat ttccagcatt tgctgtatta ccgcaccaag acgtggatgc
      421 agaagcaaac tgagaatgtt tttctcagta ttaacctgtg caatccactc acgcaccgcg
      481 agggtaaaga atgtagcgca gatctggcta cacagtgcat ccactcccgg cattgccgat
      541 ctggattcct gttgtaaaaa cgggatcgcc tccctcagcc agttatattc cacgctgtgg
      601 ttaaccggtg ctaaaaacag cgtttccggc aaagacgtta aaaaataacg tgccgaatgt
      661 tgcagccgaa gagtaccaca gacaatacag gtcgattcat tatcgacatg actcaggcga
      721 tgagctgaat tttgtggtag caggaccaca tttcccgggc gtaatgtaaa aatctctccc
      781 gtcggcattt ccagcttcgc cgctccttgc gttaacgcat gccaacgaat aactgataat
      841 tccccggcac catgtggaag ctgccagtca cttcctaaca cgcaattctt atcgatcgtt
      901 ccttgtggag cgttaagcat caacaaacgg ctaagggcat ccatatcaga ctcctgagtg
      961 gtttttgatt acaaacacca acaatcttag taaaca
//
LOCUS       DBFIFI010000467          994 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.473, whole genome shotgun sequence.
ACCESSION   DBFIFI010000467 DBFIFI010000000
VERSION     DBFIFI010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.473"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(112..801)
                     /locus_tag="N7B39_005097"
     CDS             complement(112..801)
                     /locus_tag="N7B39_005097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HGU2424858.1"
                     /translation="MNTQYLQYVREQLMAATADLNGATKGQLEAWQEHAQFDTGTYKR
                     KKPRILDVVTGKMITLDNTPTSGKQSYAKGSSIALVSPVEFSTSSWRRAVLSLDEHQK
                     AWLLWCYSESVRWGHQVTITQWAWSEFKDLLSNRKIAGKTLDRLKTLIWLAAQDVKSE
                     LAGREAYEYQTLASLVGVTTKNWSETFTERWVAMKHIFLQLDSDALLLVTRTRSKQKA
                     AFLQQNIAKLD"
     gene            complement(816..938)
                     /locus_tag="N7B39_005098"
     CDS             complement(816..938)
                     /locus_tag="N7B39_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000971054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HGU2424859.1"
                     /translation="MMSESYISELLRCRWGLLCLCRFPDSVLNDYRMLKNYAKI"
BASE COUNT          264 a          252 c          191 g          287 t
ORIGIN      
        1 gcagccagca tgtttaccgt acaagtacaa ctcagggcat aaaaaaaccc actcggcagc
       61 gggtttatac attttttaca acataccaaa tttgcatgaa gtatatggct tttaatccag
      121 ttttgcaata ttttgctgta aaaatgctgc cttttgtttt gaacgtgttc tcgtcacaag
      181 caataaagca tcactatcaa gctgtagaaa aatgtgcttc attgcaaccc agcgttcagt
      241 aaatgtctcg gaccagtttt ttgttgtcac tcccaccaat gatgccagtg tctggtattc
      301 ataggcctca cgccctgcaa gttcgctctt cacatcctgt gcagccagcc agattaacgt
      361 cttcaggcga tccagtgtct tacctgcaat ttttctgtta cttaacaaat ctttaaactc
      421 gctccatgcc cattgcgtta tggtgacctg atgcccccat cgaacgcttt cgctgtaaca
      481 ccaaagcaac catgctttct gatgttcatc gagagacaaa accgcgcggc gccatgaaga
      541 ggttgagaat tcaaccgggc tgaccaaagc aatggatgaa ccttttgcgt acgactgctt
      601 accggaagtc ggcgtattat ccagcgtaat catcttgcca gttaccacat ccagaatgcg
      661 cggcttcttt cgtttgtatg taccagtatc aaattgtgca tgctcctgcc aggcttcgag
      721 ctggcctttc gttgctccgt tcaagtcagc agtagctgcc ataagttgct cacgaacata
      781 ctgtaaatat tgggtattca tgcagtaaat cctttctata ttttggcata attcttcaac
      841 attcggtaat cgttcaaaac cgaatcgggg aaacgacata agcacaggag cccccagcga
      901 cagcgaagga gttctgatat ataagactca gacatcattc attccccggt tctccaatat
      961 ctgtttcact catcatccat aactacctgt aatt
//
LOCUS       DBFIFI010000468          985 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.474, whole genome shotgun sequence.
ACCESSION   DBFIFI010000468 DBFIFI010000000
VERSION     DBFIFI010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..985
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.474"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..219)
                     /locus_tag="N7B39_005099"
     CDS             complement(<1..219)
                     /locus_tag="N7B39_005099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU2424860.1"
                     /translation="MLCAVQKNLHAARKVLSGIIGNVPKNNKPKTIIFDLRFLSAIKQ
                     KVFLGSEQPLFVKHTAMMREACKELPQLV"
     gene            complement(226..>985)
                     /locus_tag="N7B39_005100"
     CDS             complement(226..>985)
                     /locus_tag="N7B39_005100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424861.1"
                     /translation="EQLFHCVNELKYGLSSNNEYDLFKNRETSLWKRKVPESAALAMT
                     GTVYKLFGSRLLNARNQLDKNLLEMAMQMVYGKDMVTKSGDILIDIQLNKDGSLQSKH
                     YTFDVGEVINSYNIDLFVNASSINQTYLHDKNPGDVISLYGTLDNIPLVVKECTGKNH
                     QINVKAKLPQERSEKVEDMCERMRGGISMFNHTTKTAGNIEHNLQLSFLVDSRPSITN
                     KYSAKDVAPDILVLPVNVYHADFKIKINNAVEKT"
BASE COUNT          273 a          207 c          160 g          345 t
ORIGIN      
        1 cactagctgc ggtaattcct tacatgcctc cctcatcatt gcggtatgct ttacaaacaa
       61 cggttgttca ctgcccaaaa aaactttctg cttaattgca ctaagaaaac gtaaatcaaa
      121 aatgattgtt tttggtttat tattttttgg tacattacct attatcccgc taagaacctt
      181 ccttgctgca tggagatttt tttgcacagc acataacaca attgactaag tcttctctac
      241 tgcattattg attttaattt taaaatccgc atgatatacg ttgacgggca atacaagtat
      301 gtctggcgct acatctttgg ctgagtattt attggtgatc gacggccgtg aatccacgag
      361 aaaacttagc tgcaaattat gctctatatt accagctgtt tttgtggtgt gattaaacat
      421 agagatgcca cctctcattc tttcacacat atcctcaact ttttcacttc gttcttgtgg
      481 caatttggct ttaacgttaa tttgatgatt tttacccgtg cattccttaa ctacaagagg
      541 aatgttatct aaagtgccat acaggctaat aacatcacca ggatttttat catgtaagta
      601 agtttgatta atactcgaag cattcacaaa gaggtcaata ttataactgt ttataacttc
      661 acctacatca aatgtgtagt gcttactctg gaggctgcca tccttattta attgtatatc
      721 aatcaatata tccccggact ttgtcaccat atctttgcca taaaccattt gcatagccat
      781 ttcgaggaga tttttatcta actgattgcg ggcgttgagt aagcgactac caaataattt
      841 atatactgtt cccgtcattg ccagcgctgc gctttctggt actttgcgtt tccacagtga
      901 ggtttctcta tttttaaaca ggtcatattc attattactc gacaaaccat acttcagttc
      961 gttaacacaa tggaacagtt gttcc
//
LOCUS       DBFIFI010000469          984 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.475, whole genome shotgun sequence.
ACCESSION   DBFIFI010000469 DBFIFI010000000
VERSION     DBFIFI010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..984
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.475"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            193..>984
                     /gene="tdcA"
                     /locus_tag="N7B39_005101"
     CDS             193..>984
                     /gene="tdcA"
                     /locus_tag="N7B39_005101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HGU2424862.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPV"
BASE COUNT          267 a          182 c          216 g          319 t
ORIGIN      
        1 tcattttttt tgacaaaaat cagggtttat gctgattttt atactttaat ttattgatat
       61 ttaaaggtat ttaattgaca tgacgatacc ctggaaagta ttgaaagtta atttgtgagt
      121 ggtcgcacat atcctgttca tttcattttg atacacttca tgccgtcaat gaggtaatta
      181 acgtaggtcg ttatgagcac tattcttctt ccgaaaacgc agcacctggt agtctttcag
      241 gaagtcatta gaagtggttc tatcggctcg gctgcaaaag aattagggtt aactcaaccg
      301 gccgtcagta aaatcattaa cgatattgaa gattattttg gtgtggaatt agtggtgcgg
      361 aaaaataccg gagtaacatt aacacctgcc ggtcaattgt tactctctcg ttccgaatcc
      421 attacccgtg aaatgaaaaa tatggttaat gagataagcg gtatgtcttc tgaggcggtg
      481 gtggaagtct catttggttt tccttcattg attggtttta cttttatgtc agggatgatc
      541 aacaagttca aagaggtgtt cccgaaagcg caggtttcta tgtatgaagc gcaactgtct
      601 tcgttcttac cggcaatccg cgacggtcga cttgattttg cgattggtac gttaagcgca
      661 gaaatgaagc ttcaggattt acatgttgag ccgctgttcg agtccgagtt tgtgctggta
      721 gccagtaagt cccgaacatg caccggcacc accacgctgg agtcgttgaa gaacgaacag
      781 tgggtgttgc cacaaactaa tatggggtac tacagcgaac tgcttactac gttacaaaga
      841 aatggcatca gtattgaaaa catcgttaaa accgactcag tcgtgacaat ttataatctt
      901 gttctcaatg ctgatttctt aactgtaatt ccttgtgata tgacgtctcc ttttggttct
      961 aatcaattta ttactattcc ggtt
//
LOCUS       DBFIFI010000470          980 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.476, whole genome shotgun sequence.
ACCESSION   DBFIFI010000470 DBFIFI010000000
VERSION     DBFIFI010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..980
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.476"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            98..>980
                     /gene="escD"
                     /locus_tag="N7B39_005102"
     CDS             98..>980
                     /gene="escD"
                     /locus_tag="N7B39_005102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscD"
                     /protein_id="HGU2424863.1"
                     /translation="MLSSYKIKLLNGAMRNRELQLPMGNLTLGTEDNDIVYFPLEQGL
                     NQFLLDIREEGVFLLSPVEFWIDGQPTPYEADKPLPVGKVIDIAGCCFIIGDIDHSLP
                     LSDVPERFSAKSRRKKRLILASVIGATFALSGAIGSYVLLSPKAEPPTFTRADVYQQL
                     KENKLHAITLVWHGKNIALYGRCESTTDLTPFFNYLKEKNIFYYNNIICNNQIISAIN
                     DVLTEYGYKDIIITKGNKPGFFLLSGYIPPSPKWSEVENLLLNTPGVAGWEIHNNSNN
                     KINELASEFKKNKLINYV"
BASE COUNT          312 a          196 c          180 g          292 t
ORIGIN      
        1 actgtacaac agcttccttt ctactctatt cttagacgat gtaagttcac catatttttt
       61 ctcattgttc aaccaccagg atgaataaaa tttaaaaatg ttatcctcat ataaaataaa
      121 acttctcaat ggtgcgatgc gtaatcggga attacaactt ccgatgggaa acctaacgct
      181 aggtacggaa gataacgata ttgtttactt tccccttgaa caaggcctca atcagttttt
      241 acttgatata cgtgaggaag gtgttttcct tttatctccg gttgaatttt ggattgatgg
      301 gcaacccacg ccctatgaag cagataaacc attacctgtc ggaaaggtta ttgatatagc
      361 gggatgttgc ttcatcattg gtgatattga ccattccttg ccattgtctg atgtgccaga
      421 aaggttctct gccaaaagtc gacgcaaaaa acgccttatc ctggcgagcg tgattggtgc
      481 tacttttgcg ttgtctggtg ccatcggcag ttacgttttg ctttccccaa aagctgaacc
      541 cccaacgttt accagagctg atgtatatca gcagctaaaa gaaaacaaac ttcatgcaat
      601 cactctggta tggcatggga aaaatatcgc gctttatggg cgttgcgaat caaccacgga
      661 tttaactcca ttttttaatt acctaaaaga aaaaaatatt ttttattaca ataatatcat
      721 ttgcaataac caaatcatat ccgcaattaa tgatgtctta acggaatacg gttacaaaga
      781 tatcattatt acaaaaggta ataagcctgg attctttctg ttatcgggat acattccccc
      841 ttcacccaaa tggagcgagg ttgaaaatct gttactgaat acgccaggag ttgcaggatg
      901 ggaaatacac aacaacagta acaataaaat caatgaactg gcatcagagt ttaaaaagaa
      961 taaactaata aactatgtca
//
LOCUS       DBFIFI010000471          972 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.477, whole genome shotgun sequence.
ACCESSION   DBFIFI010000471 DBFIFI010000000
VERSION     DBFIFI010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..972
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.477"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..654)
                     /gene="queE"
                     /locus_tag="N7B39_005103"
     CDS             complement(<1..654)
                     /gene="queE"
                     /locus_tag="N7B39_005103"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HGU2424864.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     "
     gene            809..>972
                     /locus_tag="N7B39_005104"
     CDS             809..>972
                     /locus_tag="N7B39_005104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HGU2424865.1"
                     /translation="MKRDLNTLRIFIVIIFIFNLSGCGYNDIQTYDEQVNASWSEVLN
                     QYQRRADLIP"
BASE COUNT          257 a          236 c          213 g          266 t
ORIGIN      
        1 tttatgggtt tgcatcgaca aacgccaatt acgcgcaatg caggtttcaa tgcacaaacg
       61 tgtggcatca tccttttggc taatcggctg cagtgcaatg actcgcggtt tatcatcggt
      121 cagcgtcgcc aaaagttcat ccagtgcttc aatatcacgt acgcgtccca ctggatgctt
      181 gatttcgttg gctcgctcca gtgcctgtga caacacttca tagccgccgc gcatgttcag
      241 ctttggcgat acggtaaccc aggtattcgg tgtacagcgt acttcatgag taccactggt
      301 ttcgatctgg cagctaaaac cgttcttttc gagcaggtca gtcagtggca gcaaatcatg
      361 aatgcaaggc tcaccacccg taatcaccac atgccgcgcg gtatatccct ggcgaccaat
      421 gacagccagc aagtcttcac tgctcgccgc cccccactta tcactctctt tggtcttcgc
      481 cagaatgctg aaaagggaga cttcccgatc ctcaagcttt tcccaggtgt gtttggtgtc
      541 gcaccaggca cagccaaccg ggcatccctg taaacgaata aaaatggcgg ggacgccggt
      601 aaagtaaccc tcaccttgca gggtctggaa catctcgtta atcgggtact gcatagcatt
      661 ctctgtgaag tggataattg ttaattattg cagatcctgc cacaacaatc atgtcttatt
      721 aacattctgt tacaggcagg tttatggcaa aaagtggatt agatcataaa gaataaaatg
      781 aagcagagta ttatgtttta aggaattaat taaaagggat ttaaacacgc tcagaatatt
      841 catcgtaatt atatttattt tcaatttatc aggctgtgga tataacgata ttcaaaccta
      901 tgatgagcag gttaacgcct cctggtctga ggtattgaat caatatcaac gtcgcgccga
      961 tcttattcct aa
//
LOCUS       DBFIFI010000472          970 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.478, whole genome shotgun sequence.
ACCESSION   DBFIFI010000472 DBFIFI010000000
VERSION     DBFIFI010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..970
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.478"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..>970
                     /locus_tag="N7B39_005105"
     CDS             <1..>970
                     /locus_tag="N7B39_005105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HGU2424866.1"
                     /translation="LDRLVQQRGFDGRTQRYHYDLTGKLTQSEDEGLVTLWHYDESDR
                     LTHRTVNGEPAEQWQYDEHGWLTEISHLSEGHQVAVHYGYDDKGRLAGERQTVHNPET
                     GELLWQHETEHAYNEQGLANRVTPDSLPRVEWLTYGSGYLAGMKLGGTPLVEFTRDRL
                     HRETVRSFGNNAYELTSTYTPAGHLQSQRLNSQVYDRDYDWNDNGDLVRISGPRQTWE
                     YGYSATGRLESVRTLASDLDIRIPYATDPAGNRLPDPELHPDSTLTAWPDNRIAEDAH
                     YVYRHDEYGRLTEKTDRIPAGVIRTDDERTHHYHYDSQHRLVFYTR"
BASE COUNT          224 a          293 c          319 g          134 t
ORIGIN      
        1 cgcttgaccg gctggtacag cagcgcggct ttgacgggcg gacgcaacgt taccactatg
       61 acctgaccgg aaaactcacg cagagtgaag atgaggggct tgtcaccctc tggcactacg
      121 acgaatcgga ccgcctcact caccgcacgg tgaacggcga accggcagag cagtggcagt
      181 acgacgagca cggctggctg acagaaatca gccacctgag cgaaggccat caggtggcgg
      241 tgcattacgg ttatgatgat aagggccgcc tggccgggga gcgccagacg gtgcataacc
      301 cggagacggg ggaactgctg tggcagcatg agacagagca cgcatacaac gaacagggtc
      361 tggcaaaccg cgtcacgccg gacagcctgc cgcgggtgga gtggctgacc tacggcagcg
      421 gttatcttgc ggggatgaag ctgggcggga cgccgctggt ggagttcacg cgcgacaggc
      481 tgcaccgcga gacggtgcgc agcttcggca ataacgcata cgaactgacc agcacataca
      541 ctcccgcagg ccatttacag agccagcgcc tgaacagcca ggtgtatgac cgtgactacg
      601 actggaatga caatggcgac ctggtgcgca tcagcggccc gcgacagacg tgggaatatg
      661 gctacagtgc cacgggcagg ctggagagcg tgcgcaccct tgcatcagac ctggatatcc
      721 gcatcccgta tgcgaccgac ccggcgggaa accggctgcc ggacccggag ctacacccgg
      781 acagcacgct cacggcgtgg ccggataacc gcatcgcgga ggatgcgcac tatgtctacc
      841 gacacgatga atacggcagg ctgacggaga agacggaccg catcccggcg ggtgtgatac
      901 ggacggacga cgagcggacc caccactacc actacgacag ccagcaccgc ctggtgttct
      961 acacgcggat
//
LOCUS       DBFIFI010000473          968 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.479, whole genome shotgun sequence.
ACCESSION   DBFIFI010000473 DBFIFI010000000
VERSION     DBFIFI010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..968
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.479"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(1..553)
                     /gene="sctT"
                     /locus_tag="N7B39_005106"
                     /pseudo
     CDS             complement(1..553)
                     /gene="sctT"
                     /locus_tag="N7B39_005106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503299.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
     gene            complement(555..815)
                     /locus_tag="N7B39_005107"
     CDS             complement(555..815)
                     /locus_tag="N7B39_005107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
                     /protein_id="HGU2424867.1"
                     /translation="MDDIVFAGNRALYLILVMSAGPIAVATFVGLLVGLFQTVTQLQE
                     QTLPFGVKLLCVSICFFLMSGWYGEKLYSFGIEMLNLAFARG"
     gene            complement(826..>968)
                     /locus_tag="N7B39_005108"
     CDS             complement(826..>968)
                     /locus_tag="N7B39_005108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HGU2424868.1"
                     /translation="LTLGMMMMSPVTISTPIKLILFVAMDGWTMLSKGLILQYFDLSI
                     NP"
BASE COUNT          342 a          203 c          160 g          263 t
ORIGIN      
        1 cgtaaacgat agtatgagtc attatattat taatatatga aaataaaggc tctaatttag
       61 gaaaattaaa acttcctgaa ggccataaat tataactttg ctaaagagtt tccagaataa
      121 agttcaatcc accgttagta agataaaccg ctgcagagaa aaggttaaat aatcttgcta
      181 attccgaggt atcgacacct gtagccggat ccaaagtact acttaacgtt gcaccgcgtt
      241 ggttatcgat aaagctacct atagcaagga aaatccaaaa tggagatgct aataagcaag
      301 caatgaacag ccctatgata atttctcttg cagctaacaa agcgatatcg atttcattta
      361 aggtcgataa ttcatagtgg taatgctgaa ctaatgcaca tgataccact attattattg
      421 gatgacggac tactgttggt atattcccac tggcgaaaaa tggtaacaaa tagaaagttg
      481 gtgccaatcg gcaaaaaccg agcgctgtag cagccagtag actatgcaac tgatagagta
      541 tcacctcccc catgctatcc tctcgcaaat gctaaattca gcatttcaat accaaaagaa
      601 tataattttt caccatacca tccgctcatt aaaaaaaagc aaatacttac gcacaataat
      661 ttcacgccaa atggcaacgt ctgttcttgc aactgcgtta ccgtttgaaa aagccctacc
      721 aataaaccaa caaatgtcgc tactgcaata ggtcctgcag acataactaa aatcagatac
      781 agcgccctat tccctgcaaa aacaatgtca tccatatttt tttcctcagg gattaatgga
      841 taggtcaaaa tattgtaata tgagtccttt tgataacatt gtccaaccgt ccatcgcgac
      901 aaacagaatt aattttatcg gtgtagatat ggttaccgga ctcatcatca tcatacctaa
      961 cgttaaca
//
LOCUS       DBFIFI010000474          966 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.480, whole genome shotgun sequence.
ACCESSION   DBFIFI010000474 DBFIFI010000000
VERSION     DBFIFI010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..966
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.480"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(92..>966)
                     /gene="cusS"
                     /locus_tag="N7B39_005109"
     CDS             complement(92..>966)
                     /gene="cusS"
                     /locus_tag="N7B39_005109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HGU2424869.1"
                     /translation="VISILIVFIVLLAVHKGHAPIRSVSRQIQNITSKDLDVRLDPQT
                     VPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTPITNLITQTEIALSQSRSQ
                     KELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKKMLNLADEVGKVFDFFEAL
                     AEDRGVELQFVGDECQVAGDPLMLRRALSNLLSNALRYTPPGEAIVVRCQTVDHLVQV
                     IVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLAIVKSIVVAHKGTVAVTSN
                     ARGTRFVIVLPERG"
BASE COUNT          226 a          259 c          249 g          232 t
ORIGIN      
        1 tcattgcttt atatgatagg tggcttgttt ataatgatgg aaatttagcg aaaaacgccg
       61 taaaccctcg ccctgaattt gaacttaata atcaccccct ctccggcaac acaatcacaa
      121 accttgtccc ccgcgcattt gacgtaaccg caaccgtgcc tttatgcgcg acaacaattg
      181 atttcactat cgccagccca atgccgctac cttcgccttt tcgttggcgg gaggggtcaa
      241 cgcggtagaa acggtcaaac aatcgcggta agtgctcggg cgcaatgggc gtaccggggt
      301 tttcgacgat aacttgcacc agatgatcga ccgtctggca gcgcactaca atagcctctc
      361 cgggtggcgt ataacgcagg gcgttcgaaa gcaggttgct taacgcccga cgcagcatca
      421 gcggatcgcc cgcaacctgg cactcgtcgc caacaaactg cagctccacg ccgcgatctt
      481 ccgctaaagc ctcgaaaaaa tcgaacactt tgccgacttc atccgccagg ttgagcattt
      541 tcttttcggg gattaactgg ttgttatcgg cctgggcgag aaacagcata tcgctgacca
      601 ttttcgccat tcgcgtcagc tcttcgagat tagagtagag cacatcttcc agctccttct
      661 ggctgcgcga ctggctgagg gcgatttccg tttgcgttat gagattagta attggtgtgc
      721 gaatttcgtg ggcgatatcc gctgagaaat tggactggcg ggtaaagaca tcctcaatac
      781 gctcgatcat atggttgaac gacagtacca gctgttccag ctcaatcggc acggtctgcg
      841 ggtcaaggcg gacgtcgaga tctttcgagg tgatattctg gatttgacgg ctgacgctgc
      901 ggatcggcgc gtgaccttta tgaaccgcca atagcacgat aaagacgatc aggatgctga
      961 tgaccg
//
LOCUS       DBFIFI010000475          965 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.481, whole genome shotgun sequence.
ACCESSION   DBFIFI010000475 DBFIFI010000000
VERSION     DBFIFI010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..965
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.481"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(331..566)
                     /locus_tag="N7B39_005110"
                     /pseudo
     CDS             complement(331..566)
                     /locus_tag="N7B39_005110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(725..>965)
                     /locus_tag="N7B39_005111"
     CDS             complement(725..>965)
                     /locus_tag="N7B39_005111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001310425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HGU2424870.1"
                     /translation="LQCPITLAIPQRGVFLRNAEGSRVCSLYDEMALSRIINDGMHHP
                     LSREPITLSMLVAREQCEFDCSIGHFTVRSDCYSV"
BASE COUNT          282 a          231 c          178 g          274 t
ORIGIN      
        1 tacgttattc ggcagagttc gtatgtacta cggcgtccca accatgtccc aaaacaaaaa
       61 tttctgccct atacacgttc caaaaaattc aacagcaaag agagtctgct gacgagttgt
      121 tgattcactg attaatacgt aaaattgata actgctggaa atcatacaat actcgtactt
      181 tcgaaagttc gctaaccagg tgctacacgc gaggtgttgc agttttcttt acgatttatc
      241 attcaatcct aatccatgca tgacatgtgg tgcctgtggg gtgatcctac ctacgtaatg
      301 tggacacagc cctaatcaag gttctgtttt tcaaaacagt tccgtgctga gacagccaca
      361 agtactgtgt cggcaccacc gattgtaatc acactagatc taattaaaca cagccgtctg
      421 cattatttcc cgactcataa aatattagcc gtaaatgcat tcgactttta gtgagtggaa
      481 gaaactttcc gccctagcat tatcgtaaca gcgccccttt gcactcatcc tgtcatgcag
      541 gtatgccgct tcagaagcgc ctgataatgc gttgaaatca ggacggtggc tacttgctat
      601 tcagttcacg atacatccct taaggaccag cgaatagcaa tgagtcactt tattatcaag
      661 tttgatgacc gctttgatgc taacttttaa taagagtctc ttcacacaaa atcgtgtacc
      721 gagtctacac tgaataacaa tcactcctca ccgtaaagtg accgatactg caatcaaact
      781 cacactgctc tctggccaca agcattgata atgttattgg ctctctgctt agtgggtgat
      841 gcatcccatc attaattata cgagaaagag ccatttcatc atataaacta catactctgg
      901 aaccttcagc atttcttaaa aaaacacctc gttgaggaat tgccagcgta atcgggcact
      961 gtaat
//
LOCUS       DBFIFI010000476          964 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.482, whole genome shotgun sequence.
ACCESSION   DBFIFI010000476 DBFIFI010000000
VERSION     DBFIFI010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..964
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.482"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            172..705
                     /locus_tag="N7B39_005112"
     CDS             172..705
                     /locus_tag="N7B39_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2424871.1"
                     /translation="MKKYIIPFVATTLMFSATTFAKDVDQGTLTIRGEVVDTTCYFVN
                     GDSTTDIVVEDVVTSAMNQLINNEIYTLKTGSTSHDLEIQCDGNTAPSIEVLASEFDA
                     NNFTLNKGTAENIGFAIFLDDGTLTRMRPGLKTPLKDNGSGQYFLNLNAQYARTSGNP
                     VTKGSVESTVTIKISAD"
     gene            755..>964
                     /locus_tag="N7B39_005113"
     CDS             755..>964
                     /locus_tag="N7B39_005113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HGU2424872.1"
                     /translation="MRIITALLMSFFILPVHAGVVIYGTRIIYPEKNREVLVQLMNQS
                     KNASLIQAWIDDGNTTIAPEKIQVTF"
BASE COUNT          326 a          177 c          179 g          282 t
ORIGIN      
        1 tgctcttctt ataaagaagg gaataattaa gaatattaaa ttttcaaact ttcatcaact
       61 tcacatcaag aaattaaaaa atttaaacat taatctaaat aataacaacc tgatcacata
      121 atcgggtttg aaaacgagtg accttccata ccagttaatt aaggatgatt tatgaaaaag
      181 tatattattc ccttcgttgc gacaacgttg atgtttagcg caacaacctt cgccaaggat
      241 gtcgatcaag gaacactaac cattagagga gaagttgttg atacaacgtg ttactttgtt
      301 aatggtgatt cgacaacaga tatcgttgtc gaagatgttg taaccagtgc aatgaatcaa
      361 cttatcaata atgaaatcta tactttaaaa accggttcaa cctctcatga cttagaaatt
      421 cagtgtgacg gaaatacagc cccaagtatc gaagtactag catcagagtt tgatgctaat
      481 aactttacgc tgaataaagg aactgctgag aacattggct ttgccatttt cctcgatgat
      541 gggactttga ccagaatgag accagggtta aaaacaccgc tgaaagataa tggctctggt
      601 caatattttc tcaatttaaa tgcacaatat gcccgcacaa gtggcaatcc tgttacaaaa
      661 ggctccgtag agtcaaccgt aacgattaaa atcagtgcag attaacgttt tttgccggta
      721 gctctgctac cggtattatt tacggagtga ttacgtgaga ataataacag cattactgat
      781 gtcatttttt attttacctg ttcatgccgg tgtggtcatc tatggtacgc gcattattta
      841 tccggaaaaa aacagagaag ttctcgtaca gttaatgaat caaagtaaaa atgcctcttt
      901 gattcaggca tggatcgatg atgggaacac cactatcgcg ccagaaaaaa ttcaggtcac
      961 attt
//
LOCUS       DBFIFI010000477          958 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.483, whole genome shotgun sequence.
ACCESSION   DBFIFI010000477 DBFIFI010000000
VERSION     DBFIFI010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..958
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.483"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(474..692)
                     /gene="ydfC"
                     /locus_tag="N7B39_005114"
     CDS             complement(474..692)
                     /gene="ydfC"
                     /locus_tag="N7B39_005114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HGU2424873.1"
                     /translation="MQKREPVIIAPDYTDDELYEWMHQKINAAQDLKWANEARAKQAE
                     NLSALEQDITNLEKAAALSIARMITYPR"
     gene            complement(764..>958)
                     /locus_tag="N7B39_005115"
     CDS             complement(764..>958)
                     /locus_tag="N7B39_005115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424874.1"
                     /translation="TEDIVNDFVIELRGLIFSYKVTTIFADGSRETVRALRLKGCVKD
                     FATTFWARKLDCIHNQFPLE"
BASE COUNT          220 a          248 c          229 g          261 t
ORIGIN      
        1 cccataacac tgtttttggt tttaactgtt ccgcgtgcgc tcagccgcat tcaccgcatc
       61 acaaaattca ctttaaaaag ggcggcagag cagtcacgga gtaaaactga taccgccaaa
      121 cgtcaccaga aaattgataa cagagggcgt tgcagcgggg ttgtcactta agcgtatggt
      181 caacctgaca acccggtgtc ctcaacgggg gaaggaataa cccctccata cttaccgccg
      241 cgccatttcg cggattgcca caaccggaag cgcacggtcg acgaaaattt aacgacaggc
      301 tatctatgaa ccagctacct cgccgtgcgc tttcgcgtta tggtctgact tttcagggaa
      361 atatcctttc agtaaactgt cagtgccgta tgctcacccg tgtccggcgc acgcactcca
      421 cctcacccgt ggagaactcc ttaattacca accttagctt cgttggttag ctattaacgc
      481 gggtatgtaa tcattctggc aatgcttaat gccgctgctt tttccagatt ggtgatatcc
      541 tgctccagag cggacagatt ttcagcctgc ttagccctgg cttcattggc ccatttcaga
      601 tcctgcgctg cattaatttt ctggtgcatc cactcataaa gttcatcatc ggtatagtct
      661 ggcgcgatga tgacgggttc tcgtttctgc atgtcggctc cttgtggtta gcgttgcctg
      721 cttttaacca cgtcaggcga ggtggtatcc tctgaggggt ctgttactcg agaggaaatt
      781 ggttatgaat acaatcaagt tttcttgccc agaatgtggt ggcgaagtct ttgacacatc
      841 ctttaagccg cagggctctg acagtttcgc gggagccatc tgcaaaaatt gtggtcacct
      901 tgtaactgaa gatgagtcct cgcagttcga tgacgaaatc gttgacaata tcttcggt
//
LOCUS       DBFIFI010000478          958 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.484, whole genome shotgun sequence.
ACCESSION   DBFIFI010000478 DBFIFI010000000
VERSION     DBFIFI010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..958
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.484"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..843
                     /locus_tag="N7B39_005116"
     CDS             <1..843
                     /locus_tag="N7B39_005116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HGU2424875.1"
                     /translation="LSVNRLVYFSLLKKLFQAMNLCQIPGRAGALLTTLLRHISTING
                     FDNLMAQPLLSDEPLTALMDHYLDTDALADGLPLYVSLYPTEGGMQDIIDCIRAELGV
                     GTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQGTMFGDGGMGGWRNMQGNT
                     PVTPLVDAGCNMVIVTHLSDGSLWDRQAFPDTTILEIRPRKRLKYAGDGGNSGGLLSF
                     TSAHTDAWRQQGYEDTMLAMEHIRKPLAARQALTRSEAVLQKSLDITEEADLALRNAM
                     ARIK"
BASE COUNT          228 a          234 c          267 g          229 t
ORIGIN      
        1 ctgtcggtaa acagattggt ttacttttca ttgctgaaaa aattgttcca ggcaatgaac
       61 ctctgccaga tccccggacg tgcaggagca ctgcttacga cgcttcttcg ccatatatcg
      121 acaatcaacg ggtttgacaa tctgatggct cagccgttgt tgtcagatga gcccctgaca
      181 gcgctgatgg atcattatct tgatactgat gctctggcag acgggctacc gctgtatgtg
      241 tcgctgtacc ccacagaagg gggcatgcag gatattattg actgcattcg tgctgaactg
      301 ggtgtcggaa ccacgaaaaa cgccgttttt cagcatatcc agagcctgcc ccgcggacag
      361 cagaaagagg ctctgcttgc gtcagccgcg ctgcccctgc tgttccgtcc ccgtgaggtt
      421 caggggacaa tgttcggtga tggtggtatg ggaggatggc gaaatatgca gggaaatacc
      481 cctgtgacgc ctctggtcga tgccggatgc aatatggtga ttgtgacgca tctgagtgac
      541 ggttctttat gggatcgcca ggcttttccg gacaccacaa tccttgagat ccgtccccgg
      601 aaaaggctga aatatgcagg tgatggtggc aacagcggcg gtctgctcag ttttacatcg
      661 gcacataccg acgcctggcg tcagcagggc tatgaagaca cgatgctggc gatggagcat
      721 atccggaaac cgctggcagc acgtcaggca ctgacccggt cagaggcggt attgcagaaa
      781 agcctggata taacggaaga ggcagatttg gcactgagaa acgcgatggc ccggattaaa
      841 taaagacgcc ccaaagaaac gccaccgcct gtggtggtgt ttttcctgtc tggtgttatt
      901 gaaaatattt tcagatatat attacctgag agaaagtttt acccctcacc tgtcaatt
//
LOCUS       DBFIFI010000479          956 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.485, whole genome shotgun sequence.
ACCESSION   DBFIFI010000479 DBFIFI010000000
VERSION     DBFIFI010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..956
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.485"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..197)
                     /locus_tag="N7B39_005117"
     CDS             complement(<1..197)
                     /locus_tag="N7B39_005117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HGU2424876.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSS"
     gene            complement(223..597)
                     /gene="tomB"
                     /locus_tag="N7B39_005118"
     CDS             complement(223..597)
                     /gene="tomB"
                     /locus_tag="N7B39_005118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HGU2424877.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
BASE COUNT          279 a          196 c          193 g          288 t
ORIGIN      
        1 gaggaaggga tcttgtcgta cagtttattc atggtcaatt cggcgaggcg gtgatctgcg
       61 gctgagtaaa ataccgccag ttcattatct gataattcgt atttattttt ctcgataaca
      121 cgctccagcg tgtcaattgt ctggcaacga cgtaaacgca ttaaataatc ggttttcgtt
      181 aaaggttttt cggacatact tctacccatg gttgtaataa ttctaacaag ataaactcgc
      241 aggattctct ttcgtcgcat tgacaaaaca acggaatagt cgattacctg acttccgcca
      301 tttctgaaga tcctgcatat taataccata actactgaac aacataaagg tgtcatccag
      361 atattcgtcg atctgctcaa tgagcttatt gtcttcatta tacttaattt tgtaattaag
      421 tgcgaaggtc gcaatatgct caatcagttc attcaactgg aggttgatcg ccgaggttgg
      481 gtcgttaacc cagccatgat tgctttcttc aaggtttgca aggcagtcat gatacagagt
      541 ttcacagaga aacttaagct gtgcgatatc atgtcttttg ggtgagtatt catccataac
      601 gcgtcccctt cttagcggtt gaactaacgg acacctttcg ggatggaaaa aacttactga
      661 cctggacttg ccctctcgtt tgttagctta actataagcc actctttgca ggttttcatc
      721 gcattattac gaaaaattac aattaggaag gaagcgggac agggctcact ttttcatact
      781 agcaccaacc cgcagcaggt aaaagcagtt tgctcgaact gttcataaca tttcaggatt
      841 atcgccagat gaaattggtg acactaatac caggattgct ctgaatatga cgtaataacc
      901 gaggaatgaa taaagaatta ccgcaaataa ttaagaatag cctctaaatg attatg
//
LOCUS       DBFIFI010000480          954 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.486, whole genome shotgun sequence.
ACCESSION   DBFIFI010000480 DBFIFI010000000
VERSION     DBFIFI010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..954
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.486"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(1..136)
                     /locus_tag="N7B39_005119"
                     /pseudo
     CDS             complement(1..136)
                     /locus_tag="N7B39_005119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            286..954
                     /locus_tag="N7B39_005120"
     CDS             286..954
                     /locus_tag="N7B39_005120"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGU2424878.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
BASE COUNT          284 a          210 c          186 g          274 t
ORIGIN      
        1 ctactgatgt atcaacacga ttcaacaata cccgatattt attccatgcc tccagcaacg
       61 atctttcttc ctccgttgcg atttccagat ctacagcatc ctgcagtggc gcaatatact
      121 cactgaattc ctggatgtag aactgtgtgg tgacggtctt ccagccattc ggctcctgct
      181 gtatcgcagc ataccaggct atttcaatat cgctatgctg cggcagcatt taaccccttg
      241 taattcatcg ccataattga tttaattcac aaataaaact ataacatggt gaaattaatg
      301 aaaaaaaaca cagatgatgg ggctaaaatt tacacaccac ttaccctaaa gctttatgac
      361 tggtgggttt tgggagtatc aaatcgtctt gcatggggat gtcctacaaa ggaacacctt
      421 cttccacact ttctggaaca tttaggtaac aaccatctgg atattggtgt tggaactggg
      481 ttttacctta ctcacgtacc tgagagtagt ctgatatctt taatggattt gaacgaagct
      541 agcctgaacg cggcatctac aagggctggg gaatcaaaaa ttaaacataa aattagccat
      601 gatgtttttg aaccttatcc cgcggcgtta catggtcaat ttgattccat ttccatgttt
      661 taccttcttc actgcctgcc tggaaatata tctacaaaaa gctgtgtaat acgcaatgcg
      721 gcgcaggcct taactgacga tggaactcta tacggagcca caattcttgg cgatggagtt
      781 gtgcacaata gcttcggtca aaaactgatg cgcatttaca atcagaaagg catcttttca
      841 aacacaaaag attccgaaga aggcttaaca catatactct cagagcattt cgagaatgtt
      901 aaaaccaagg ttcaaggtac tgtagtaatg ttttccgctt cagggaaaaa atag
//
LOCUS       DBFIFI010000481          948 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.487, whole genome shotgun sequence.
ACCESSION   DBFIFI010000481 DBFIFI010000000
VERSION     DBFIFI010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..948
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.487"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(209..>948)
                     /locus_tag="N7B39_005121"
     CDS             complement(209..>948)
                     /locus_tag="N7B39_005121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424879.1"
                     /translation="ESSIDEKKKKAQVTQSNINKNTPQQLTDKDLFGNETTLAVSEED
                     IQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMRKYYTVSTFSGIPDRQKPLTCN
                     KNKDKNENEDVARAENMNWMQALRFVAAKGHQKAIIVYQDMLQTGKYDSALKSTVWSD
                     YKNEKLTDAISLRYLVRFTLVDVATGEWATWSPVNYESRVIFPPAGITKTSNKELSND
                     HVTEAQLFNLKQKTYVSMVKDLVSRFQ"
BASE COUNT          268 a          199 c          173 g          308 t
ORIGIN      
        1 aaatataaaa aaatgaagag ataatagcaa ttctttccat tgcagatgat actattttaa
       61 tttatgaaga attaaaaaaa taactcacat gcaatacact ggtaatatat attgtaactt
      121 tataaaaaca atcatattta cattatagaa atactcaact cctgtttgct gccaccctaa
      181 actacaggcg gcagcataac aataacgatt actggaatcg actcaccaag tctttcacca
      241 tcgatacata tgttttttgt ttcaaattaa acagttgcgc ttccgtaaca tggtcatttg
      301 atagttcttt attgctcgtt tttgttattc cagccggcgg aaaaattact ctcgattcat
      361 aattcaccgg cgaccaggta gcccactcac ctgttgccac atccaccagc gtgaaacgta
      421 ccaggtagcg cagggagata gcgtcagtga gtttttcatt tttatagtcg gaccatactg
      481 ttgatttcag cgcagagtca tattttcctg tctgcaacat atcctggtaa acaataatcg
      541 ctttctgatg tccttttgca gccacaaaac gcagtgcctg catccagttc atattctcag
      601 cacgggcaac atcctcgttc tcatttttat ctttgttttt gttacaagtc agaggctttt
      661 gcctgtccgg gataccagag aatgtggaaa cagtataata ttttcgcatc tcctcctgca
      721 taatggtttc cggtgcgcgg ttgccggatt gaacaagtat tacaggagaa ttaaggggaa
      781 cgcggaactc atctccatca agcgctgctt gaatatcctc ttcggatacg gccagagtgg
      841 tttcattacc gaataaatct ttgtctgtca gttgctgggg agtgttttta ttaatattac
      901 tttgtgtgac ttgagctttt ttcttctttt catcaataga agattcag
//
LOCUS       DBFIFI010000482          942 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.488, whole genome shotgun sequence.
ACCESSION   DBFIFI010000482 DBFIFI010000000
VERSION     DBFIFI010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..942
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.488"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            242..826
                     /locus_tag="N7B39_005122"
     CDS             242..826
                     /locus_tag="N7B39_005122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HGU2424880.1"
                     /translation="MDKRAKNQIVDSDIARLLLKLRKSRNLTVTELAQRSGVSQAMIS
                     KVERGTSSPSATILSRLANALNITLSKLFAELEMQQNSLVLLADQQQHWIDEETGITR
                     WSLSPAGACPELIKVEIPPMGQLTIPASANEHLSGQTLWMLSGSLDFRVNHQTHHLQA
                     GDCLALTFASEYQMRNPDSGQSCSYIVAFSQKNP"
     gene            complement(851..>942)
                     /locus_tag="N7B39_005123"
     CDS             complement(851..>942)
                     /locus_tag="N7B39_005123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU2424881.1"
                     /translation="QHMGFKLSGRSERDNQGRPYPLLHLYNAM"
BASE COUNT          283 a          189 c          201 g          269 t
ORIGIN      
        1 ttttaggtat ttctacagta catttgacac gatgattaac ctctatgtag ctaggttttt
       61 gtaaagaaac tgtgcgtcac tgtatccagg actgttatgt gccaaataag gaagttgaag
      121 ttaccagctc ttactctaaa gctataacgt atagaaaaaa tgcatttgag acgtgtaaaa
      181 tcatttattt aatcgcaatt tatgtaatgg cataatcagg tcggtgaaag aggggagaag
      241 aatggataaa agagctaaaa atcagatagt cgatagcgat attgcacgtt tgttactcaa
      301 gctcagaaag tctcgaaact tgactgttac agagcttgcc caacgatctg gtgtcagtca
      361 ggcgatgatc agcaaagttg aacgaggcac ttcaagcccc agtgcaacaa tacttagtcg
      421 gttagctaat gcactgaata ttacgctatc aaagcttttt gcagagcttg agatgcaaca
      481 gaattcactt gtacttttgg ctgaccagca acaacattgg attgatgaag aaacaggcat
      541 tacccgctgg tctttgtccc cagcgggtgc atgtccggaa ctaataaaag ttgaaatccc
      601 ccctatgggg caattgacta tccctgcctc tgcaaatgag catctttctg gacaaactct
      661 atggatgtta agcggcagcc ttgattttcg agtcaatcat cagactcatc atttacaagc
      721 cggtgattgt cttgctctta cctttgccag cgagtaccaa atgagaaacc cagatagcgg
      781 ccagagttgt tcttacattg tcgctttcag ccaaaaaaat ccatgatgta attctcctaa
      841 aattatcatg tcacattgcg ttataaagat gtaatagagg gtagggtctg ccttgattgt
      901 cccgctcgga acgtcctgaa agtttaaagc ccatatgttg at
//
LOCUS       DBFIFI010000483          925 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.489, whole genome shotgun sequence.
ACCESSION   DBFIFI010000483 DBFIFI010000000
VERSION     DBFIFI010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..925
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.489"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..128)
                     /locus_tag="N7B39_005124"
     CDS             complement(<1..128)
                     /locus_tag="N7B39_005124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HGU2424882.1"
                     /translation="MHRDSERSRRIRGWVIMKKDLKTLALARLSGFRHKTVKVPEWG"
     gene            complement(95..811)
                     /locus_tag="N7B39_005125"
     CDS             complement(95..811)
                     /locus_tag="N7B39_005125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HGU2424883.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMDKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDALYAFKVL
                     FPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRLKGKPVSFVVPLAFVKNLDKTLTVN
                     TGALLTMSVSANGGTPPYKYAWKKDGQPVDGQTTDTFSKPGAQSADAGKYTCVVTDSA
                     EKAQSVTSVECTVTVSAAAG"
BASE COUNT          210 a          276 c          221 g          218 t
ORIGIN      
        1 ccccattccg gcaccttcac cgttttatga cgaaaccctg acagtctggc cagcgccagc
       61 gttttcagat cctttttcat gatgacccat ccccttatcc ggcggctgcg ctcactgtca
      121 cggtgcattc aacagacgtc acactctgtg ctttctctgc cgaatcggtc accacacagg
      181 tatatttccc cgcatcagcg gactgcgcac ctggcttact gaaggtgtct gtcgtctgcc
      241 cgtcaaccgg ctgaccatcc ttcttccagg cgtatttata cggcggcgtt cccccgttgg
      301 cactgactga cattgtcagc agcgcaccgg tattcacggt aagtgtctta tccagatttt
      361 tcacaaacgc cagcggtacc acaaaggaca ccggtttgcc tttcagacgc agtgagaacg
      421 ttgcagccac cacgccgttg gtaccggatg accaggtgtg ctggcgcact tccgccagga
      481 atttaaagcc cttaccggac ggaaacagca ccttaaacgc atacaacgcg tcattgtcat
      541 aggcatcacg cagggcgttc tgggcctgat tcagataaaa attgcccgac atggaaatct
      601 cagacgacgc ccccagacca ttgatgttct cctgctctgt ggagcagagc gtggtcacat
      661 caatatcctg tttctgcccg gcggtgaact gcacttcctt gatggtgcag tccaggcgca
      721 gatattccgc cttatccata gtttcagcag tcgccggggc agatgaaatc atcacctgcg
      781 tcagctgtga gcgttcatac aaagcagaca ttctgcctcc tgataataaa aaacccgcac
      841 gcggcggggt atgggttttg tagaaaaaag aaaaagtcac accgtgacct gaaactccag
      901 ggttgcacgg taacagcggt tttcc
//
LOCUS       DBFIFI010000484          916 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.490, whole genome shotgun sequence.
ACCESSION   DBFIFI010000484 DBFIFI010000000
VERSION     DBFIFI010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..916
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.490"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            280..587
                     /locus_tag="N7B39_005126"
                     /pseudo
     CDS             280..587
                     /locus_tag="N7B39_005126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(827..>916)
                     /locus_tag="N7B39_005127"
     CDS             complement(827..>916)
                     /locus_tag="N7B39_005127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector EspF"
                     /protein_id="HGU2424884.1"
                     /translation="PPPTGPSGLPPLAQALKDHLAAYEQSKKG"
BASE COUNT          283 a          205 c          176 g          252 t
ORIGIN      
        1 aacaaaaccc cttttaatcc cttatatatc aggctttctt tgtgtacagg ggtcaaccga
       61 ctcaacctgt atcaagttgc ttttgaggat actttagggg gtattatttc ctcttgaaat
      121 attagcaccc cataatggga aaaaggtaat acataacaat ggtttttaac agtatctttg
      181 ttatacaagg gggcattgag gatatcagga aaaatctcaa aatcggtacg gacaggaaac
      241 tacgtgggca gcaaaaacgt atggtaacac cgggaaatgc aacgcccacc agtcgggcta
      301 atccaccact ccaatcgtgg ttcgcagtac tgcacacacg attaccgggc tatacaggag
      361 cggtttggtc tgaaaacatc catgccgcgt aaaggtaacc gttacgacaa cacctcaatg
      421 aaaaacttat agtaaacgct gaagaatgaa actatcgttt taataaccgg gatgaagcca
      481 tctaagtaat acggaaatac attgagattt tctacaacca tcagcgccgc cactcccgtc
      541 tggggaatat ctccctggta gccttcagga aaaatataac cagataactg cgtaaaaaaa
      601 gaacaaatgg tggtgtccgc tattgccagt atacctgagt ttactgcctt tcagattatc
      661 acaatcaata gcgaacagac ttttaagctc tgtgcgcttt taacaaaacg tccctttctc
      721 cactttcacc gtataataat acattattta ttacccctcc aataaaaacc gatattacta
      781 atcacattta ttaccacaca atttgatatc ggtttttatt agttggttac cctttcttcg
      841 attgctcata ggcagctaaa tgatctttta atgcctgtgc aaggggcggt agtccactag
      901 gccctgtcgg tggcgg
//
LOCUS       DBFIFI010000485          909 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.491, whole genome shotgun sequence.
ACCESSION   DBFIFI010000485 DBFIFI010000000
VERSION     DBFIFI010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..909
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.491"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..80)
                     /locus_tag="N7B39_005128"
     CDS             complement(<1..80)
                     /locus_tag="N7B39_005128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HGU2424885.1"
                     /translation="MAKDLKTLALARLSGFRHKTVKVPEW"
     gene            complement(86..802)
                     /locus_tag="N7B39_005129"
     CDS             complement(86..802)
                     /locus_tag="N7B39_005129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HGU2424886.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMEKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDTVYAFKVQ
                     FPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRLKGKPVSYVVPLAFVKNLEKTLTVN
                     TGALLTMSVSVNGGTPPYKHAWKKDGQPVEGQTTDTFSKANTQSGDKGAYTCEVTDSA
                     EQPQSITSDACTVTVNGAGG"
BASE COUNT          206 a          265 c          214 g          224 t
ORIGIN      
        1 ctccattccg gcaccttcac cgttttatga cggaaccccg acagtctggc cagcgccagt
       61 gttttcagat cttttgccat aagccttatc cgcccgcacc attaaccgtt actgtacacg
      121 catcagaggt aatgctctgc ggctgttctg cagaatccgt tacctcgcag gtataagccc
      181 ccttatcacc tgactgcgta ttggctttac tgaaagtgtc agtagtctgt ccctctaccg
      241 gctgaccatc cttcttccag gcgtgtttat aaggcggcgt tcccccgttg acactgactg
      301 acattgtcag cagcgcaccg gtattcacgg taagtgtctt ctccagattt ttcacaaacg
      361 ccagcggtac cacataggac accggtttac ccttcaggcg aagtgaaaac gttgcagcca
      421 ccacgccgtt ggtaccggat gaccaggtgt gctgacgcac ttccgccagg aacttaaagc
      481 ccttaccgga cggaaactgc accttaaacg catacaccgt gtcattgtca taggcatcac
      541 gcagggcgtt ctgggcctga ttcagataaa aattacccga catggaaatc tcggacgacg
      601 cccccagacc gttgatgttc tcctgctctg tggagcagag cgtggtcaca tcaatatcct
      661 gtttctgacc ggcggtgaac tggacttcct tgatggtgca gtccaggcgc agatattccg
      721 ccttctccat ggtttcagca gtcgccgggg cagatgaaat catcacctgc gtcagctgtg
      781 aacgttcata caaagcagac attctgcctc ctgataataa aaaacccgca cgcggcgggt
      841 tatgggttct gttgaaaaaa ttacaccgtg acctgaaact ccagggttgc acggtaacag
      901 cggttttcc
//
LOCUS       DBFIFI010000486          903 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.492, whole genome shotgun sequence.
ACCESSION   DBFIFI010000486 DBFIFI010000000
VERSION     DBFIFI010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.492"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..>903
                     /locus_tag="N7B39_005130"
     CDS             <1..>903
                     /locus_tag="N7B39_005130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424887.1"
                     /translation="TQFEVIKAEFYINEDGSGEGYNWDENIQKAYSLVTADLYDHTEI
                     ETDNISNAYPDYDSVADNIVQGLPLFRDKAAPEINEKLKWIETGKLDNFPANKIKHQD
                     PLVARFLTHTESLSPAVMLQLLQHPDDQTRESMAQSWPAQQMHWLTDELIKDEAVARG
                     LVKNSNISADVNKKLMSVPVESVQLEQARQDNLSPDIVASLSHSPFLSVRKTLLSHYD
                     YAWLVPTAVADELINNEDPELRERITGADLTAQQAVMLSKDKSLKVREALARTLTELK
                     ITQLSATLRTEDIERIAEQMYLDN"
BASE COUNT          289 a          188 c          201 g          225 t
ORIGIN      
        1 aacgcaattt gaagtcatta aagctgaatt ttatatcaat gaagatggaa gtggtgaagg
       61 atataactgg gatgagaata ttcaaaaagc ctattcactg gtgactgccg atctttatga
      121 tcacactgaa atcgaaaccg ataacatttc caatgcgtat cctgactatg atagcgtcgc
      181 agacaacatc gttcagggtt taccgctttt tcgagataaa gccgctcctg aaattaatga
      241 gaaattaaaa tggattgaaa cggggaaact cgataatttc ccagcgaata aaataaagca
      301 tcaagatcca ctggtggcac gttttctgac tcatacagag agtttatcac ccgccgttat
      361 gctgcaactg ttacagcacc ccgatgacca gacgcgtgaa tcgatggcgc agtcctggcc
      421 cgctcaacag atgcattggc ttaccgatga attaattaaa gatgaggctg ttgccagagg
      481 gctggttaaa aacagtaata tttccgccga tgtgaataaa aaattaatgt ctgtacccgt
      541 cgagagcgta cagctggagc aggccagaca ggataatctt tctccagata ttgttgcgtc
      601 gttaagtcat agcccctttc tcagtgtacg caagacatta ctcagccatt acgattacgc
      661 ctggctggtt ccgactgcag tggccgatga gttgataaat aacgaagacc cggagttacg
      721 tgaaagaatt accggagcag atttaaccgc acaacaagca gtgatgttga gtaaagataa
      781 atcactgaaa gttcgtgaag cattagcccg gacgcttact gaattaaaaa ttacccaact
      841 gtcagcaacc ctgcgtacag aggatattga acgtatcgca gaacaaatgt atctggataa
      901 taa
//
LOCUS       DBFIFI010000487          902 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.493, whole genome shotgun sequence.
ACCESSION   DBFIFI010000487 DBFIFI010000000
VERSION     DBFIFI010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.493"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            143..>902
                     /locus_tag="N7B39_005131"
     CDS             143..>902
                     /locus_tag="N7B39_005131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR-type extended signal peptide-containing
                     protein"
                     /protein_id="HGU2424888.1"
                     /translation="MNRIYRVIWNCTLQVFQACSELTRRVGKTSTVNLRKSSGLTTKF
                     SRLTLGVLLALSGSVSGASLEVDNGQITNIDTDVAYDAYLVGWYGTGVLNILAGGNAS
                     LTTITTSVIGGNEDSEGTVNVLGGTWRLYDSGNNARPLNVGQSGTGTLNIKQKGHVDG
                     GYLRLGTQAAGVGTVNVEGEDSVLTTELFEIGSYGTGSLNITDKGYVTSSIVAILGYQ
                     ANSNGKVVVEKGGEWLIKNNDSSIEFQIGNQGTGE"
BASE COUNT          248 a          154 c          235 g          265 t
ORIGIN      
        1 tattagcaag ttagccaaat tgacactttc ccacctccaa ctaacactca atacagacct
       61 ctgacaattt aataaatttc atttttaatg tctgacgact catctctttc gtggctgtat
      121 tgtttgtatt tggagcgttt atatgaatag aatctatcgc gtgatatgga attgcactct
      181 acaggtattt caggcctgct cggaattaac tcgcagggta ggtaaaacat caacggttaa
      241 tttgcgaaag tcctctggac tgacaacgaa attcagtaga ttgacgctgg gtgttttgct
      301 ggcactaagc ggttcagtgt ctggtgcgag tctggaagtt gataatggtc agattaccaa
      361 tattgatact gatgttgctt atgatgccta cctggttggc tggtatggca ctggagtgct
      421 taatattttg gctggcggta atgcctcctt aaccactatt actaccagcg tcattggcgg
      481 taatgaggat tcggagggta ccgttaatgt tttgggtggc acctggcgat tgtatgatag
      541 cggaaataat gcaaggcctt taaatgtggg tcaatccgga acggggacgc tgaatattaa
      601 acagaagggt cacgtcgatg gaggctattt aagattaggt actcaagctg caggcgtcgg
      661 gacggttaat gttgagggag aggactcggt tttgacgacc gaattattcg aaatagggag
      721 ttacggtacg ggctcattaa atattacgga taagggttac gtcacgagtt caatagtcgc
      781 cattttagga tatcaagcga acagtaatgg taaggttgtc gttgaaaagg gtggcgagtg
      841 gctaataaaa aataatgatt cctcaattga atttcaaatt ggtaatcaag gaactgggga
      901 gg
//
LOCUS       DBFIFI010000488          900 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.494, whole genome shotgun sequence.
ACCESSION   DBFIFI010000488 DBFIFI010000000
VERSION     DBFIFI010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.494"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(725..>900)
                     /locus_tag="N7B39_005132"
     CDS             complement(725..>900)
                     /locus_tag="N7B39_005132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HGU2424889.1"
                     /translation="GVFLSDTNPTLSEYYNLNRAIYWMEFAVNNGNIDAKSKLQELKK
                     LKRMDRRKNKENP"
BASE COUNT          272 a          189 c          146 g          293 t
ORIGIN      
        1 tattgctttg tctgccaaat ctaactctct ctgaaattgt cttgcagtaa ctgcactcgc
       61 agaaatagag aaaaaaatac aattgttaat aaccttgcca tattttttag tccgtaactg
      121 ttaatgtgct atctggctct gaaaatatta actcatttaa tttttgcatg tcattagata
      181 aaccagttat acaaccctcg gaacagaatc gaggtcctaa agacggacca aaagcgttta
      241 aacaactatg tattgaaaat ccattgcgat tataagttcc tgtattaact gacggtacta
      301 atcgtatgcc gtcaggtttt ctattgcttg ctcccgggtc attaacattc caactccata
      361 ggccttgaga tattggccat ctatttataa atgtgtacaa cggggattat ttttacattg
      421 catattatta ccagaagcga ctggaatact aagcacatca taaaaattat catcacatgg
      481 tatatctgaa acacacgaaa gcatacctaa agttatgctg tataaacagg tggctaatcc
      541 caaggggatc gattccagaa atcgggttca acggatactg ataaaaattc cacctacctt
      601 ccagcccaat cggagcctga gtgttatacc gcccctgctg cggttaatgt taccgataat
      661 agttatagta cagccctgat tcatcatcat actactgctg cggcagtctg atgaactatt
      721 gcttttacgg attctcctta ttcttccttc tatccattcg ctttagcttt ttgagctctt
      781 gaagtttgct ctttgcatca atattaccgt tatttacagc gaattccatc caataaatag
      841 ctctattcaa attataatat tctgataatg ttggatttgt atctgataaa aagaccccat
//
LOCUS       DBFIFI010000489          895 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.495, whole genome shotgun sequence.
ACCESSION   DBFIFI010000489 DBFIFI010000000
VERSION     DBFIFI010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.495"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(1..153)
                     /locus_tag="N7B39_005133"
                     /pseudo
     CDS             complement(1..153)
                     /locus_tag="N7B39_005133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            247..510
                     /locus_tag="N7B39_005134"
                     /pseudo
     CDS             247..510
                     /locus_tag="N7B39_005134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            550..670
                     /locus_tag="N7B39_005135"
                     /pseudo
     CDS             550..670
                     /locus_tag="N7B39_005135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            667..819
                     /locus_tag="N7B39_005136"
                     /pseudo
     CDS             667..819
                     /locus_tag="N7B39_005136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          254 a          211 c          214 g          216 t
ORIGIN      
        1 cggcgcaggc tttccgtcac ggtttttact ttaaaatggt aaccctgggc acgcagttca
       61 tccatcaggc gttgtgcatg accgggtaaa agcaacgagg acaacgctgt cgcagtgctg
      121 acggaactgc cgacgtgtac ttatccttat ccgccagtga tataagacgg taattcacca
      181 tttggattgt ccgctccacc caacatgttg tttccttgag gttctcacac cagaaaggac
      241 atcagcatgc tgagcagaga ggacttttac atgataaagc aaatgcgcca gcaaggtgcg
      301 tatattgttg atattgcgac tcagattggt tgctctgaac gaacggtcag acgttacctg
      361 aaatacccag aacctccggc cagaaaaaca cgccacaaaa tggccgaaat ggactatatc
      421 gacatgcgtc tggctgagaa tgtctggaac ggcgaagtta tcctggcgga aatcaaagcg
      481 atgggttata ccggtggccg ttccatgttg cttcgccagt tcaggaagac accggagcag
      541 cgctttacgc aggaacacag tctgttcttc cggttgctga accgtcgata tgaaaaagcg
      601 agcattgtgc tgacgtccaa taaagggttc gccgactggg ggagagatgt tcggtgataa
      661 cgtgctggca aaatattcac ccaacgcatt gagcgtaata atctgacgct acgcacccgc
      721 attaagcggt tgactcgtaa aaccatctgc ttctcacgct cagttgagat ccacgaaaaa
      781 gtgatcggag cgtttatcga aaaacacatg ttctactaat tggatgcacc accgaataca
      841 ttaatttata taacaacatc aaaatacatt ttaggttgtc gctaatcaaa aatta
//
LOCUS       DBFIFI010000490          881 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.496, whole genome shotgun sequence.
ACCESSION   DBFIFI010000490 DBFIFI010000000
VERSION     DBFIFI010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.496"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..>881
                     /gene="espK"
                     /locus_tag="N7B39_005137"
     CDS             <1..>881
                     /gene="espK"
                     /locus_tag="N7B39_005137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspK"
                     /protein_id="HGU2424890.1"
                     /translation="VCHCYISGVPESVRYLEIKGSATDSIKNVPNGLSSLSINSYNPE
                     NQARIDNLISPSLKTLSLTGCSNIILPEKLPESVTSVTIHAEQKTTWNIGVEGMPDGL
                     DLDLQNVLLSPDVVKAKNITFQGNALDVALHFREGDIVYGLSSPREKLVNSIKLVNDF
                     SKKDIITQNTLTNAVWDPRTPRKYKQDPLIKRALNEHERGIKFKQHLKNHNNYNVTMA
                     DLSVYNRDKLWAKTSKAGLEFQTLTRNKTVIFCADELVNSLKLIANKSEGYGQSITAS
                     ELRWIYRNKDNNQIMKN"
BASE COUNT          312 a          191 c          174 g          204 t
ORIGIN      
        1 gtttgccact gctatatatc tggagtgcca gagagtgtcc gctaccttga aataaaaggt
       61 agcgccacag acagcataaa aaatgttcca aacgggttat catctctcag catcaatagc
      121 tataacccgg agaatcaggc cagaattgac aacctgatat caccgtcact gaagacgcta
      181 tcgctgactg gatgtagcaa tattatactg ccggagaaac ttccggagag tgtgacatcg
      241 gtaaccattc atgcggagca gaaaaccacg tggaacatcg gtgttgaagg gatgcctgat
      301 gggctggatc ttgatttaca aaatgtacta ctctctccag atgtagttaa agcaaaaaac
      361 atcacctttc agggcaacgc tctggatgtg gccttacact ttcgcgaggg agacattgtc
      421 tatggactat cttcacccag agaaaaactt gtcaacagca ttaaactagt taacgacttt
      481 tccaaaaaag atattataac tcagaatacg ttaacaaacg cagtatggga ccccagaaca
      541 cctcgcaaat ataagcaaga tccacttatc aaaagagcat taaatgaaca cgaaagagga
      601 ataaaattta aacaacactt aaagaatcac aataattata atgttaccat ggccgacctt
      661 tccgtataca atcgcgacaa attatgggca aaaacaagca aggccggcct agagtttcag
      721 acattaacac gcaataaaac ggttattttt tgtgcggatg agcttgtcaa ctcactcaaa
      781 ctcatagcta acaagtcaga gggctatggc cagagtatta ccgccagcga attacgatgg
      841 atttaccgta ataaagacaa caaccaaata atgaaaaaca t
//
LOCUS       DBFIFI010000491          878 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.497, whole genome shotgun sequence.
ACCESSION   DBFIFI010000491 DBFIFI010000000
VERSION     DBFIFI010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..878
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.497"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            157..726
                     /locus_tag="N7B39_005138"
     CDS             157..726
                     /locus_tag="N7B39_005138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HGU2424891.1"
                     /translation="MNMMAVPFHGNSLYVVNHNGEPYVPMKPVVAGMGLAWQSQLAKL
                     RQRFASTITEIVMVAEDGKQRNMVSMPLRKLAGWLQTINPNKVKPEIRDKVIRYQEEC
                     DDVLYEYWTKGFVVNPRKMSVMEELNQACADMKRDKNIASVFATGLNEWKQVKAAHVS
                     KIRTLVNEANMLIDFVLADTGKGKITKAD"
     gene            726..872
                     /locus_tag="N7B39_005139"
     CDS             726..872
                     /locus_tag="N7B39_005139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424892.1"
                     /translation="MGWLMISDKLITLVKSLCVLVGISFLVMLVAIFFSTAWRVLTLS
                     GLVG"
BASE COUNT          236 a          185 c          229 g          228 t
ORIGIN      
        1 ccgtaatgat gcctttgtca tttctgcgca tctcacgcgc atctcacaac acagaacctt
       61 tcaggatgac ccttgaggat accggtttgg ctgtcggtgc ctttctgtgg gctggattcc
      121 tgtgagacaa ggttcatcac taaaaggaaa taaccgatga atatgatggc cgtgccgttt
      181 cacggcaact ctctttatgt agttaaccat aatggcgaac catacgttcc catgaaacct
      241 gtcgttgcgg ggatggggct ggcctggcaa tcacagttgg ctaagttaag acagcgtttt
      301 gcgtcaacta taacggaaat cgttatggtt gctgaggatg ggaaacaacg caatatggtg
      361 tccatgccac ttcgaaaact tgccggctgg ctacaaacca ttaatcccaa caaagtaaaa
      421 cccgaaatcc gcgataaggt catccggtat caggaagagt gcgacgatgt tctttacgag
      481 tactggacga agggttttgt cgttaatccc cgtaaaatga gcgtgatgga agaactcaac
      541 caggcttgtg ctgacatgaa acgggataaa aacattgcca gtgtgtttgc taccgggctg
      601 aatgagtgga aacaggttaa agccgcgcat gtatcaaaaa tccgtacgct ggtaaatgaa
      661 gcgaatatgc tgattgattt tgtcctggct gatacaggca aagggaaaat aacaaaggcg
      721 gattgatggg gtggctaatg atatcagata aactcataac gctggtgaag agcctctgtg
      781 tacttgtcgg catttcattt ttagtcatgc tggttgccat tttcttttcc accgcctggc
      841 gagtcctgac gttatcggga ctggtggggt gaaagaga
//
LOCUS       DBFIFI010000492          870 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.498, whole genome shotgun sequence.
ACCESSION   DBFIFI010000492 DBFIFI010000000
VERSION     DBFIFI010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.498"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..>870
                     /locus_tag="N7B39_005140"
     CDS             <1..>870
                     /locus_tag="N7B39_005140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HGU2424893.1"
                     /translation="KIREGWQPYGGPFSSYTDDGAALIQAIVAEGDVSTPVVVKPSDG
                     EGTVISATRDPEYYFVVVLAGQSNSMAYGEGLPLPETYDRPDPRIKQLARRSTVTPGG
                     AACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGTVGQGLHIAKKLLPFIPANAG
                     ILLVPCCRGGSAFTTGADGTYSDASGASENSTRWGVDKPLYKDLIGRTKAALKKNPKN
                     VLFAVVWMQGEFDFGGTPANHAAQFGALVDKFRADLADMAGQCVGGSAGGVPWICGDT
                     TYFWKQKNEATYQ"
BASE COUNT          198 a          205 c          280 g          187 t
ORIGIN      
        1 aaaaaattcg tgaaggatgg cagccatacg gcgggccgtt ttcttcgtat acggatgatg
       61 gcgcagcact tattcaggcg attgtcgcag aaggtgatgt gagcacacct gttgtggtga
      121 agccgtcgga tggagagggc acagtaatca gcgccaccag agacccggag tattactttg
      181 ttgtggttct ggcggggcag tcaaacagca tggcatatgg tgaaggcctt ccgctgccgg
      241 agacatatga ccgtccggac ccgcgcatta agcagctggc gcgtcgcagt acggtgacac
      301 cgggcggtgc cgcctgtaag tataacgaca tcattccggc ggaccattgt ctgcatgatg
      361 tgcaggacat gagccgcctt aaccatccga aagcggacct gtcaaagggg cagtacggta
      421 ccgtggggca ggggctgcat attgccaaaa aactgctgcc gtttataccg gcgaatgcgg
      481 gcattctgct ggttccgtgc tgtcgtggtg gttcagcgtt caccaccgga gccgatggca
      541 catacagtga cgcgagtggt gcctcggaga attcaacccg ctggggtgtg gacaagccgc
      601 tgtataagga ccttatcggt cgaacaaaag cagcactgaa gaagaatccg aaaaatgtgc
      661 tgtttgccgt ggtgtggatg cagggggaat ttgattttgg cggtacgccg gcaaatcacg
      721 cagcacagtt tggtgcgctg gttgataaat tccgtgcaga cctggcggat atggcaggtc
      781 agtgcgtcgg tggctctgct ggcggtgttc cctggatatg tggagatacg acgtatttct
      841 ggaagcagaa gaacgaagcc acctaccaga
//
LOCUS       DBFIFI010000493          869 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.499, whole genome shotgun sequence.
ACCESSION   DBFIFI010000493 DBFIFI010000000
VERSION     DBFIFI010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..869
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.499"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..>869
                     /gene="ehxD"
                     /locus_tag="N7B39_005141"
     CDS             <1..>869
                     /gene="ehxD"
                     /locus_tag="N7B39_005141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter subunit
                     EhxD"
                     /protein_id="HGU2424894.1"
                     /translation="TDFPGGEDNAGGEHFQRVSSLIKEQFMTWQNRKNQKQITLNKKI
                     VERDAALARVSLYEHQVSQEGRKLNDFKYLLNKKAVSQHSVMEQENSYIQAKNEHAVW
                     LAQVSRLEKEIELVREELALETNIFRSEIIEKHRKSTDNIVLLEHELEKNRQRKASSF
                     IKAPVSGTVQELNIHTEGGVVTTAETLMIIVPDNDILEVTASVLNKDIGFIQPGQEVV
                     IKVDAYPYTRHGYLTGKVKNITADSVSVPDTGLVFNVIISVDRNDIQGERKKIPVTAG
                     MTVMAEIKTGVRS"
BASE COUNT          312 a          132 c          199 g          226 t
ORIGIN      
        1 taacagattt tcctggcgga gaagataatg cgggaggtga acattttcag agggttagct
       61 cattaataaa agagcaattc atgacatggc agaacagaaa aaatcaaaag cagatcacat
      121 taaataaaaa aatagttgaa cgggatgcag cgcttgctcg tgttagcctg tatgagcatc
      181 aggtatcaca ggaaggaaga aaactcaatg attttaagta tttgttgaat aaaaaagctg
      241 tttctcaaca ttcagttatg gagcaggaaa atagctatat tcaggcaaaa aatgaacatg
      301 cagtctggct tgcacaggtt tctcgacttg aaaaagaaat agaacttgtg cgggaagaac
      361 tggcactgga gacgaatatc ttcagaagtg aaattatcga gaagcacaga aaatcaacag
      421 ataacattgt gttgctggag catgagcttg aaaaaaacag gcagagaaaa gcatcatctt
      481 ttattaaagc tcctgtgagt ggtactgttc aggagttaaa tatacataca gaaggtggtg
      541 ttgtcacaac agcagaaacg ctgatgatta ttgtccctga taatgatatt ctcgaagtaa
      601 cagcatctgt acttaacaag gatatcggtt ttatacaacc tggacaggag gtcgttatta
      661 aagtagatgc atatccatat acacgtcatg gttaccttac agggaaagta aaaaatataa
      721 ctgcagattc tgtttctgtt ccggatacag ggcttgtatt taacgtgatt atatctgttg
      781 atcggaatga tatacaggga gaaagaaaaa aaatccctgt tacagctgga atgactgtta
      841 tggcagaaat aaaaaccggg gtgcgtagt
//
LOCUS       DBFIFI010000494          860 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.500, whole genome shotgun sequence.
ACCESSION   DBFIFI010000494 DBFIFI010000000
VERSION     DBFIFI010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..860
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.500"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(92..655)
                     /gene="fimA"
                     /locus_tag="N7B39_005142"
     CDS             complement(92..655)
                     /gene="fimA"
                     /locus_tag="N7B39_005142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HGU2424895.1"
                     /translation="MKLKHVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLILDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
BASE COUNT          258 a          199 c          167 g          236 t
ORIGIN      
        1 attcacattg gtgaattgct cgggtgtgcg tagccatgcc acacacaccc tgttttaatt
       61 tcagatgccg tatagccacc ttaatattga attatagata ttgaactttg aaggtcgcat
      121 ctgcgtttgc agtaccagct acggactgcc cgagagcaat ataacgagcc tggaatggta
      181 gtatattcgt gccatcaatc aagtcggttt tagcactaaa agtggcccca tcaagtatta
      241 atacctcacc cgtacggtcc aaaatttgaa taccgacatt ttgggcagag cctgccgctg
      301 aactctgcag cgctaacgta tcgtcattac tggtgacagt agtacccagg aatgcaacag
      361 ctgcattact ggaaacattg gtatcacaat cattcagctt gatattgaag ccaacggcgg
      421 agctgaggtc acccgcttta gccaggcgag aagaacgaac ctgacccagc tcaaccgttt
      481 ggttcattga ttcggaatca atcgcacaag cggcatttac cacttcacct ttaaaatgaa
      541 taacaccgcc attaacagtg gtcgttactg attcatcacc ctcggctgcg cttaccgcag
      601 cagacgacat cgccaacaca gaaacgacaa tcataccaac atgtttgagt ttcataattc
      661 tccaaaattt atatttaagt agctgaataa agagagttaa caattaagtc attctgttat
      721 tgcttataaa acaagaatca ttcttgattg gttgttgccc tgagaatata gtttccaggg
      781 ttattacatt tcacgaagaa cgataatatc tttaaatcac aaggacacct aatcacaaca
      841 agtttattgc tgcacttgtt
//
LOCUS       DBFIFI010000495          859 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.501, whole genome shotgun sequence.
ACCESSION   DBFIFI010000495 DBFIFI010000000
VERSION     DBFIFI010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..859
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.501"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(240..395)
                     /gene="hokD"
                     /locus_tag="N7B39_005143"
     CDS             complement(240..395)
                     /gene="hokD"
                     /locus_tag="N7B39_005143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HGU2424896.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVF
                     TAYEPEE"
BASE COUNT          218 a          202 c          214 g          225 t
ORIGIN      
        1 aaaacaccgt ccgcgcctgc tggcaaagac ggcagaatca tcagaattgt ataatttggt
       61 atcgtgcgcc atcggttgtc tctgctggcg cagcaggtgc cagttgttca ggctggcgtg
      121 cgaattgtaa accagaatgc caggaaaaaa caaaacccgc cgaagcgggt taagtgcggg
      181 tgcgttgagg atgcctgatt catcagaggt ggcgagggat ttctcccccg ccaggtctct
      241 tactcctcag gttcgtaagc tgtgaagaca gcgacctccg tctggccggt tcggactcgt
      301 acctcgcaga ggtctttcct cgttaccagt gccgtcacta tgacggttaa acagatgacg
      361 atcagggcga ttaacatcgc cttttgctgc ttcatagcct gcttctcctt gcctttcggc
      421 acgtaagagg ctaacctaga tttgccgttc atagattgag cctcagatta atgttaggcg
      481 tcttgcagga cgcgtaatgt taactggggc ttttctctat ctgccgttgg tgttcatgcc
      541 cgaggcagat agcctcaagc acccgcagct attctactta acttcccatt acctcgccaa
      601 tatgaaatcg gtcagaaaga cagacgtccc ataaggacaa caacggaaaa agagattttg
      661 cttgatgcta tttacctctg caccatgaag ttgcgccaaa atcccagtgg tgataacagg
      721 aatttggttg ttggtgattg gggaaagaag tacgttagac tgtgggttag ttcattgaca
      781 tgatagttac ctgccttcaa tttcctaagc aagcgatgta aacgataatc aatatcaaat
      841 gtattgacag gtaaaataa
//
LOCUS       DBFIFI010000496          845 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.502, whole genome shotgun sequence.
ACCESSION   DBFIFI010000496 DBFIFI010000000
VERSION     DBFIFI010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..845
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.502"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..386)
                     /locus_tag="N7B39_005144"
     CDS             complement(<1..386)
                     /locus_tag="N7B39_005144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HGU2424897.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGAENNRVLREATRAGANVLKEE
                     VVSRAPVRRGKLRRNVVVLSRCSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSE"
     gene            complement(383..733)
                     /locus_tag="N7B39_005145"
     CDS             complement(383..733)
                     /locus_tag="N7B39_005145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HGU2424898.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDHVLNVVGVPVPDAT
                     GGRLEILCRLGGEK"
     gene            complement(743..>845)
                     /locus_tag="N7B39_005146"
     CDS             complement(743..>845)
                     /locus_tag="N7B39_005146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424899.1"
                     /translation="RNPDLAEREDLVQGELPFSVSVLIYDLRCPTVL"
BASE COUNT          184 a          292 c          196 g          173 t
ORIGIN      
        1 tgttcactgc gcacatcaaa tgccgggcgc acaaacgggt gcggtggcat attcacggtc
       61 cccatttcca caaaccgcca gtagaaagca ttgcgcgggt tatccgcctt catggtgtta
      121 tcgctgttac cggtgtccgg attaacaccc cggatatgca caccggattc catcccgcca
      181 tcgcgggagc accgggaaag gaccaccaca ttgcggcgaa gttttcccct gcgtaccggt
      241 gcccgtgaca ccacttcttc tttcagcaca ttcgcacccg cacgggttgc ctcacgcagc
      301 acccggttat tttccgcacc actcagaagc tgcaaatcgc ggctgatgtc ctccagcccc
      361 gaaaaatcca gcagggtttc gatcattttt cccctcccag ccgacagaga atttccagac
      421 gcccgccggt tgcatccgga accgggaccc cgacgacatt caggacatgg tcacgccagg
      481 gaccactcag cacatgaagt cgtgacgccg ccgtgatttc acgaccagac tgaccgcgta
      541 cccagatgcg gatttccgcc tgcgccattt ccgcaccgga ctgcatccgc tcccggctgc
      601 tcctgccccg gatatccgca tgaattttcc cgcatgacac ccattcttcc gtcatttctc
      661 cggcagcatt acgggttaac accgggttca gaacactgat catctgtgtc agacgacctg
      721 cagatattgc cattccccct cctcataaca ccgtcggaca acgcaaatcg taaatcagca
      781 cggaaacaga aaacggcagt tccccctgca cgaggtcttc ccgctcagca agatccggat
      841 tccgg
//
LOCUS       DBFIFI010000497          842 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.503, whole genome shotgun sequence.
ACCESSION   DBFIFI010000497 DBFIFI010000000
VERSION     DBFIFI010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.503"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..>842
                     /locus_tag="N7B39_005147"
     CDS             <1..>842
                     /locus_tag="N7B39_005147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HGU2424900.1"
                     /translation="RRTGKRVWRRGRDLTGWMSLSRKPEVTWYGWDGDRLTTVQTDTT
                     RIQTVYEPGSFAPLIRIETDNGEREKAQRRSLAEKLQQEGSEDGHGVVFPAELVRLLD
                     RLEEEIRADRVSSESRAWLAQCGLTVEQLARQVEPEYTPARKVHLYHCDHRGLPLALI
                     SEDGNTAWSGEYDEWGNQLNEENPHHLHQPYRLPGQQYDKESGLYYNRNRYYDPLQGR
                     YITQDPIGLEGGWSLYAYPLNPVNGIDPLGLSPADVALIRRKDQLNHQRAWDILSDTY
                     EDMK"
BASE COUNT          208 a          201 c          300 g          133 t
ORIGIN      
        1 cgccggacgg ggaaacgggt gtggcgtcgc gggcgtgacc tgacgggatg gatgtcgctg
       61 tcgcggaaac cggaggtgac gtggtacggg tgggacggcg accggctgac gacggtacag
      121 accgacacca cgcgtatcca gacggtatac gagccgggaa gcttcgcgcc gctcatccgc
      181 atcgaaacag acaacggtga gcgggagaaa gcgcagcgcc gcagcctggc ggagaagctg
      241 cagcaggaag ggagcgagga cgggcacggt gtggtatttc cggctgaact ggtgcggctg
      301 ctggacagac tggaggaaga aatccgggca gaccgcgtga gcagtgaaag ccgggcgtgg
      361 cttgcgcagt gcgggctgac ggtggagcaa ctggccagac aggtggagcc ggaatacaca
      421 ccggcgagaa aagttcatct ttaccactgc gaccaccggg gcctgccgct ggcgctcatc
      481 agcgaagacg gcaatacggc gtggagcggg gagtatgatg aatggggcaa ccagctgaat
      541 gaggagaacc cgcatcacct gcaccagccg taccggctgc cggggcagca gtatgataag
      601 gagtcggggc tgtactacaa ccggaaccgg tactacgatc cgttgcaggg gcggtatatc
      661 actcaggacc cgatagggct ggagggggga tggagtctgt atgcgtatcc gctgaatccg
      721 gtgaatggta ttgatccatt agggttaagt cccgcagatg tagcgctaat aagaagaaaa
      781 gatcaactaa accatcaaag agcatgggat atattatctg atacttatga agatatgaag
      841 ag
//
LOCUS       DBFIFI010000498          841 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.504, whole genome shotgun sequence.
ACCESSION   DBFIFI010000498 DBFIFI010000000
VERSION     DBFIFI010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..841
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.504"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..>841
                     /gene="espX6"
                     /locus_tag="N7B39_005148"
     CDS             <1..>841
                     /gene="espX6"
                     /locus_tag="N7B39_005148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector pentapeptide repeat protein EspX6"
                     /protein_id="HGU2424901.1"
                     /translation="EIIECASPEIRSSVLNDLYSIANFLPDNNLTPNESWKRFCETCE
                     RFYVAQKSITGDKSERLTRKLSISDAGITMTFKIGDVVINTISTAIPEDATGQRCIEG
                     LNLAEMDLTDIDLSKMALRNVNFNGSILRNAKFSGTICEGVDFTDCDLRNAEFENASL
                     ENNDFRKVRHLTYVNFKNANLRNSNFNGKVLTGVTFTGSDLSNAYLEHIDFTTVILYE
                     TSKIPGIPGTPQIPGTPKVILTGAILNYSDLSGKDLSEYNLTGILCMYTNFSNANLTN
                     CKIS"
BASE COUNT          274 a          165 c          161 g          241 t
ORIGIN      
        1 ctgaaattat tgaatgcgcc tctcctgaaa taagaagtag cgtcctgaac gatctttata
       61 gcattgctaa tttcctcccg gacaataatc tgaccccaaa tgagagctgg aaaagatttt
      121 gtgagacatg cgagcgcttt tacgttgctc agaagagtat cactggagat aagagtgaac
      181 gtcttacgcg aaaactctct atctctgatg caggaattac aatgaccttc aagataggtg
      241 atgttgtcat caatactatt agcactgcta ttcctgaaga tgcaacgggt caacggtgta
      301 tcgaagggtt gaatttagca gagatggatt taaccgacat agacttgtcg aaaatggcgc
      361 taaggaatgt caattttaat ggcagcattc ttagaaatgc caagttctcc ggtacgatct
      421 gtgaaggcgt ggattttacc gattgtgatc tgcgtaatgc agaattcgaa aatgcctcat
      481 tagaaaataa tgattttcgt aaagttcgcc acttgactta tgtaaatttc aaaaacgcaa
      541 atctacgaaa cagtaacttc aacggaaaag ttctcactgg cgtaaccttt actggaagtg
      601 accttagtaa cgcgtatctt gaacacatag atttcacaac cgtgattcta tatgaaacat
      661 ctaaaatacc tggaatacct ggaacacctc aaataccggg aacacctaaa gtaattctta
      721 ctggcgcaat actaaattat tccgatctat cgggaaaaga tctttcagaa tataatctta
      781 ctggtattct ctgcatgtat accaactttt caaacgctaa tttaacaaat tgtaaaatct
      841 c
//
LOCUS       DBFIFI010000499          840 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.505, whole genome shotgun sequence.
ACCESSION   DBFIFI010000499 DBFIFI010000000
VERSION     DBFIFI010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..840
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.505"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            134..259
                     /locus_tag="N7B39_005149"
                     /pseudo
     CDS             134..259
                     /locus_tag="N7B39_005149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
     gene            300..375
                     /locus_tag="N7B39_005150"
     tRNA            300..375
                     /locus_tag="N7B39_005150"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:333..335,aa:Ile,seq:cat)
     gene            385..461
                     /locus_tag="N7B39_005151"
     tRNA            385..461
                     /locus_tag="N7B39_005151"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:419..421,aa:Arg,seq:tcg)
     gene            475..551
                     /locus_tag="N7B39_005152"
     tRNA            475..551
                     /locus_tag="N7B39_005152"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:509..511,aa:Arg,seq:tct)
     gene            complement(680..823)
                     /locus_tag="N7B39_005153"
                     /pseudo
     CDS             complement(680..823)
                     /locus_tag="N7B39_005153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309987.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          204 a          202 c          218 g          216 t
ORIGIN      
        1 ttaaaaattg cacatggcac tattagtttt ctaaatattg tgtatttttt gtattgcagg
       61 atgaccctgt aacgaagttt gcgtaacagc attttgctct acgagtttgc cagcctcccc
      121 cagtggctgg cttttttatg tccgtagcgt caaagcagca atgtcgctgg ggcgtcgtgc
      181 aattggcgtt gagctggaga ctgaacgttt tgagcagacg gtcagggaag ttcaggattt
      241 agtcagtcag aacggatgat attgcaggat tagttacgta ccgttattat cctgcgcccg
      301 gccctttagc tcagtggtga gagcgagcga ctcataatcg ccaggtcgct ggttcaaatc
      361 cagcaagggc caccatatca cataccgcca ttagctcatc aggacagagc gccagccttc
      421 gaagctggtt gcgcggggtt cgggtccccg atggcggtcc attatcggta ttcagcgttg
      481 ttagctcagc cggacagagc aattgccttc taagcaatcg gtcactggtc cgaatccagt
      541 acaacgcgcc acaccacact tatctgccct gactctcttt tgcgggcttt ttattacagg
      601 aaagacaccg gacagtgaaa tgttaaatgc ctcacaattc aggcagttga atgttgcctg
      661 acatgctgag cgtttgttaa aaaaatttcg catggtgaat ccccctgtgc ggaggggcaa
      721 tcagcaacca ggtatatgtg ataatcgcgg attcaggtgc tgatactgaa ttcaccggga
      781 ggcacccggc accatgcaag aaaaagaatg tgcatgcaaa catgcccctc tccggagggg
//
LOCUS       DBFIFI010000500          825 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.506, whole genome shotgun sequence.
ACCESSION   DBFIFI010000500 DBFIFI010000000
VERSION     DBFIFI010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.506"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..642
                     /locus_tag="N7B39_005154"
     CDS             <1..642
                     /locus_tag="N7B39_005154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HGU2424902.1"
                     /translation="SAESAQSAEQSRIAAEDAVNRIPTVVGPPGPKGEPGPAGPQGPK
                     GDKGERGDTGPAGATGERGPGGDTGPAGPQGPKGDRGERGETGLTGSTGPQGPKGDTG
                     ATGPAGPQGPKGETGAAGPVGATGPQGAKGDPGETQIRFRLGPMRIIETNSYGWFPGT
                     DGALITGLTFLDPKDATQVQGMFQHLQVRFGDGPWQDVKGLDEVGSDTGRTGE"
     gene            644..>825
                     /locus_tag="N7B39_005155"
     CDS             644..>825
                     /locus_tag="N7B39_005155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HGU2424903.1"
                     /translation="MNILRKLMQSLCGCGKHDDCENGRSLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDA"
BASE COUNT          218 a          183 c          301 g          123 t
ORIGIN      
        1 tcagcagaaa gcgcacagtc agcggaacaa agcagaatag cggcggaaga cgccgtaaac
       61 agaattccca ccgtggtggg gcctcccgga ccaaaggggg aaccgggtcc cgcgggtcct
      121 caggggccga agggagataa aggagagcgt ggagacaccg gtccggcagg ggcaaccggt
      181 gaacggggac cgggaggaga tacaggtccg gcaggtccgc aggggccgaa aggcgacagg
      241 ggagagcggg gagagaccgg tctgacagga agtacaggtc cacaggggcc aaagggagat
      301 accggggcaa caggtccggc aggaccgcag ggaccgaaag gggaaacagg tgcggctggc
      361 ccggtggggg ctaccggacc tcagggggcg aagggcgacc cgggggagac acaaatacgg
      421 ttccgtctgg ggccgatgag aattattgag acaaacagct atggctggtt cccgggtaca
      481 gatggtgcgc tcatcaccgg actgaccttt cttgacccca aagatgccac acaggttcag
      541 gggatgtttc agcatttgca ggtcagattt ggtgacgggc catggcagga tgttaaggga
      601 ctggatgaag tgggcagtga tacaggcaga actggagaat gacatgaata ttttgagaaa
      661 gcttatgcag agtctgtgcg gttgcggaaa gcatgatgac tgtgaaaacg gacggtcact
      721 tacagcacaa ttgcgactgg gaccggcaga cattctggag tcagatgaga atggcattat
      781 tccggagcag gacagggtaa tcacacaggt ggtgatactg gatgc
//
LOCUS       DBFIFI010000501          817 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.507, whole genome shotgun sequence.
ACCESSION   DBFIFI010000501 DBFIFI010000000
VERSION     DBFIFI010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..817
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.507"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            557..775
                     /locus_tag="N7B39_005156"
     CDS             557..775
                     /locus_tag="N7B39_005156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424904.1"
                     /translation="MFKYAEMLMAEEDDKVCELIFKQQDNLILKINVRFPRIIIKKYR
                     TLQGKVVWLWILPFVIEWLIVCTLGVNS"
BASE COUNT          248 a          128 c          164 g          277 t
ORIGIN      
        1 gtcaacaaac tcaggcgtct tgcctgacat gctcctgaca tcattgatat tcacgctcat
       61 ctggcatatg catttgcgct ctttatgctg caattctggc gaatgttcat ttacagcgtc
      121 atcaggatgc cgttatactc attgataaaa tactaacata taaccctgag aataatcagg
      181 gaacacgctg gttactgggg gcggaattgt tacgtgctgg caagcatggc agggctctgt
      241 acgtattgac aggacatgtc aatgaatttc ccccctactg gtacgagctt gctcttttac
      301 attttatgaa tgaggcatat attaatgctg caacggcatt tcgatatgga tttttaacaa
      361 gtatatattg ttgaaatgct ttgttagaat ctttatccat tccctagggc tgtatagtat
      421 gatttttcag gtatttctga atactgccaa atattattgc aaaatattct ctaaattatg
      481 aattaaatat ccataagcat tattgcctgg gaactggaca tataattact cttctgtgct
      541 aaaggaacgt tctgaaatgt ttaagtatgc agagatgttg atggcggaag aagatgataa
      601 agtatgtgaa cttattttta aacaacaaga taatttgatt ttaaaaataa atgttagatt
      661 tcccagaata attataaaga aataccgaac attacaaggt aaagtcgtat ggctatggat
      721 attgccattt gttattgaat ggttaattgt gtgtactttg ggtgtaaaca gttagcttgg
      781 taaaagtaaa tatcctgatt gttgtttgtt aaggagt
//
LOCUS       DBFIFI010000502          814 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.508, whole genome shotgun sequence.
ACCESSION   DBFIFI010000502 DBFIFI010000000
VERSION     DBFIFI010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..814
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.508"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            156..788
                     /gene="yqeH"
                     /locus_tag="N7B39_005157"
     CDS             156..788
                     /gene="yqeH"
                     /locus_tag="N7B39_005157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU2424905.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWTEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNSAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
BASE COUNT          268 a          136 c          156 g          254 t
ORIGIN      
        1 taacgagaaa tgcattttaa aatgtcttaa tcagaattat agcaaataca gattaatcgc
       61 aatttttccc agcaaaatta caaaatcatg gtgtattgac atcatcaatc accaatgaaa
      121 ttatgcaatt atatacggat agggaggttc ttaacatggg gctttgtagt cgttataaaa
      181 gtcttacatg caatagttgc tcaatgcatt gccaaataat gccagaagag tcaccgcgtt
      241 tgcagtactg cgcaaactcg tgtttttgta tgtggaccga agaaagttca tattttaatc
      301 gtggtgtggt agaaggtatc ttaacaaaaa accacaatgc gagattaagc ggatatattt
      361 ttgtcgattt ttcagtaagt tttttacgtc tattccttga aaaagactgg attgactatc
      421 ttgcaagtac tgacatgggg attgttttag tcagcgaccg caatatgcaa tcgttagcta
      481 attactggcg aaaacacaac tctgcaatat ctgctgtcat atataatgat gatggtcttg
      541 atgttgccaa tgaaaagatc agacaactat ttattggtcg ttatttatca tttaccagag
      601 gtaacacatt aactcagatg gaattcacca taatggggta tatggtttct ggttataatc
      661 catatcagat tgctgaagtt ctggatatgg atatccgtag catctacgcg tacaagcaac
      721 gaatcgaaaa gagaatgggt ggtaaaataa acgaattatt tattcgttca cattcggtcc
      781 aacattgata ctcaacaatc tgccagaatc atac
//
LOCUS       DBFIFI010000503          809 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.509, whole genome shotgun sequence.
ACCESSION   DBFIFI010000503 DBFIFI010000000
VERSION     DBFIFI010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..809
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.509"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..374
                     /locus_tag="N7B39_005158"
     CDS             <1..374
                     /locus_tag="N7B39_005158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HGU2424906.1"
                     /translation="QREELKARIIGLVRKNERMTISQLERATGAGWHSVRRCLVDVLA
                     CGDLYMPGKYGVFTSEQVYRVWRKAAEKATDQTLIRKLPDGEIRRYDRQQNIICGECR
                     KSEVMLRVLAFYQGNFQEAVL"
     gene            431..>809
                     /locus_tag="N7B39_005159"
     CDS             431..>809
                     /locus_tag="N7B39_005159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424907.1"
                     /translation="MTTFTKEQLISHVSENVKAMKFAVKQTAFKNSLEAIELDLALAL
                     VAQASLEAEPVLYMNRFTGKTFSLEEQPGADKEPEIYVPLYAAPPDSAAMLQAGNFRE
                     KKGSSTNNFREISETSTNYPVTLD"
BASE COUNT          240 a          169 c          216 g          184 t
ORIGIN      
        1 aacagcgtga agaactgaag gcccgaatta tcgggctggt acgcaaaaat gaacgcatga
       61 cgatatcaca actggagaga gcgacgggag caggctggca ttcagtcaga cgttgccttg
      121 tggatgtact ggcttgtggc gatttataca tgcccggtaa atacggtgtt tttacatcag
      181 aacaggtgta tcgcgtatgg cgtaaggcag cggagaaagc aaccgaccag acattgattc
      241 gaaagttacc agacggagaa atacgccgct acgacagaca acagaacata atctgtggcg
      301 agtgccggaa gagtgaagtt atgctgcgtg tactggcgtt ctatcagggc aattttcagg
      361 aggcggtact gtgagtgaat tagctatcag gcttcaattg tcgctggcat tcgcatcaaa
      421 ggagaatgag atgaccactt ttacaaaaga gcagttaatc agtcatgtta gtgaaaatgt
      481 aaaggcgatg aaatttgcag taaaacagac agcattcaaa aattctctcg aggcaattga
      541 gttggattta gcactggccc ttgttgctca ggcttcgctg gaagcagagc ccgtgcttta
      601 tatgaatcga tttaccggaa agacattctc actggaagag caacccggtg ctgataagga
      661 accggaaata tacgtgccgc tatatgctgc cccgccagac agcgccgcca tgcttcaggc
      721 tggaaacttt cgggaaaaaa agggttcgtc aaccaataat tttcgggaaa tctcggaaac
      781 gtcaaccaac tatccggtaa ctctggatg
//
LOCUS       DBFIFI010000504          808 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.510, whole genome shotgun sequence.
ACCESSION   DBFIFI010000504 DBFIFI010000000
VERSION     DBFIFI010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..808
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.510"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            178..462
                     /locus_tag="N7B39_005160"
     CDS             178..462
                     /locus_tag="N7B39_005160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HGU2424908.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAWADSLSTDNPLPVPR"
     gene            462..737
                     /locus_tag="N7B39_005161"
     CDS             462..737
                     /locus_tag="N7B39_005161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGU2424909.1"
                     /translation="MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIYVLRLWHTRENR"
BASE COUNT          210 a          190 c          210 g          198 t
ORIGIN      
        1 ggggggggta aggtgaaatc catcaatcag taccggctta cgccgggctt cggcggtttt
       61 acacctgtgc tacatgtaac ctccgtatac cgccttacat gcgctgacgc gcattatttg
      121 ccttgttccg tctgaaagta atcactatga ttaaatatga ttaacgacta atcggatatg
      181 caaatgaaaa acaataccgc acaagcaaca aaagtcatta ccgcgcatgt gccattacct
      241 atggctgata aagtcgacca gatggccgcc agactggaac gctcccgggg ttgggttatc
      301 aaacaggcgc tttctgcatg gcttgcccag gaggaggagc gtaatcgcct gacgctggaa
      361 gccctggacg atgtgacatc cggacaggtt attgaccatc aggctgtaca ggcctgggcg
      421 gacagcctca gtactgacaa tccgttaccg gtgccacgct gatggaactg aagtggagca
      481 gtaaagcgct ttctgatttg gcgcggttat atgattttct ggtgctagcc agtaaacctg
      541 ctgccgccag aacggtacag tccctgacac aggcaccggt cattctgtta actcatccac
      601 gtatggggga acagttattt cagtttgaac ccagggaggt cagacggatt tttgctggcg
      661 agtacgaaat ccgttacgaa cttaatggcc agactattta tgtattgcgc ctgtggcaca
      721 cacgagaaaa caggtagatt gtgtgtgggg acaacttaac atagcctgca gatattaatt
      781 ctggaacatt attatcaggc cagatatt
//
LOCUS       DBFIFI010000505          798 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.511, whole genome shotgun sequence.
ACCESSION   DBFIFI010000505 DBFIFI010000000
VERSION     DBFIFI010000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..798
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.511"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..252
                     /locus_tag="N7B39_005162"
     CDS             <1..252
                     /locus_tag="N7B39_005162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HGU2424910.1"
                     /translation="GASMILGGVAQMLAPKAKVPEYKSTDNGRQNTYFSSLDNMIAQG
                     NPMPVPYGEMLVGSRRISQDISTRDEGGDGKVVVIGRQR"
     gene            491..>798
                     /locus_tag="N7B39_005163"
     CDS             491..>798
                     /locus_tag="N7B39_005163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424911.1"
                     /translation="MGKGGGKAHTPVEAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETALGVEVTKAKPVTR
                     T"
BASE COUNT          217 a          170 c          257 g          154 t
ORIGIN      
        1 ggggccagca tgatactggg tggtgtggct cagatgctgg ccccgaaggc taaagtaccg
       61 gagtacaaaa gcacggataa cggcagacag aacacgtact tttcctcgct ggataacatg
      121 attgcacagg ggaacccgat gccggtgcct tacggtgaaa tgctggttgg ctcacggcga
      181 atctcccagg acatcagtac ccgtgatgaa ggcggtgacg ggaaggtggt ggttatcggg
      241 cggcagaggt aaaaagaata aaaaaatccc gcagtgaccg aaggctgcgg gaacagaaaa
      301 tgaagattaa ccacagggag ttttgttttt attggcccga aaaaactgta acgcccggga
      361 atgatatctg ccacgggggc gtacagaaaa tgtgaagata ttcagaattt ttattcagtc
      421 atgatacagg catcctccgg gatgcctgtt gtttttgtgc gtaacagtta tcacagtaaa
      481 gggtgagaca atgggcaaag gtggtggcaa ggcgcacacg ccggttgagg caaaggacaa
      541 tcttaagtcc acgcagatga tgagcgtgat tgatgcgatt ggtgaagggc cgattgaagg
      601 tccggtgaag gggctgcaga gtatcctggt gaacaaaacc ccgctgacgg acacggacgg
      661 taatcctgtg atacatggtg tgacagcggt ctggcgcgcc ggggagcagg agcagacacc
      721 acctgaaggc tttgagtcct ccggagctga aaccgcactg ggcgtggaag tgacgaaggc
      781 aaagccggtg acgcgcac
//
LOCUS       DBFIFI010000506          792 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.512, whole genome shotgun sequence.
ACCESSION   DBFIFI010000506 DBFIFI010000000
VERSION     DBFIFI010000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.512"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(1..792)
                     /locus_tag="N7B39_005164"
                     /pseudo
     CDS             complement(1..792)
                     /locus_tag="N7B39_005164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          207 a          140 c          159 g          286 t
ORIGIN      
        1 tgtagagctg ctgataggta gagcaacctt tattaagcca cgcctcaccg ccgacacgcg
       61 tcgacccatc ataattttta atttcagtcg ttgattttaa tacttggcca tcccagtaaa
      121 ctaaaggcct gctgaacggg agagcggcca tatatgccat tgctaattgt tcatcttgtt
      181 gatggttaaa tagaaagtca ttgaaaagat tgccattgtt aggaaaatcg tggttaatgt
      241 taatcaatat ccgtcttttt gatgggatgc ttgagattgt ggaactcggt tttgatgcaa
      301 gggtttggag atcgccacca aatgaaaaag cttttatcgt cttcatgagc aatgaaaaat
      361 caatggcgct taaatcatct ctttcataaa gcagcgatga catttgttct tcatccaact
      421 ggcaagttac cagatattcc atgtaattca taacggcttt ttcatgatat tttgggttaa
      481 ataagttagt attgtgtaat cgctcattat aatttttaag cggtggggtt attgatcttt
      541 ctatttgttc atgttttgag tgttttgctg catcgtaacg taaacctcgt acgccatatt
      601 ttcgcatttc acgaacgtat tgattttgca acataataac ctagtcactt ttgggggggg
      661 ttttaggcat tccatttaaa cgcttttctt ttaatgcatg gaggttttcc cattcatcct
      721 tagaaatatt atctttactt gcttcgaagt cttttgctgt gagatgtata tcttctggat
      781 ggatttgttt ac
//
LOCUS       DBFIFI010000507          792 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.513, whole genome shotgun sequence.
ACCESSION   DBFIFI010000507 DBFIFI010000000
VERSION     DBFIFI010000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.513"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            81..263
                     /locus_tag="N7B39_005165"
     CDS             81..263
                     /locus_tag="N7B39_005165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HGU2424912.1"
                     /translation="MTEHRGGAGNFAEDRQKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQNSHGGGRKSDS"
     gene            342..>792
                     /locus_tag="N7B39_005166"
     CDS             342..>792
                     /locus_tag="N7B39_005166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HGU2424913.1"
                     /translation="MQIKSVEDIFIHLLSDTYSAEKQLTKALSKLSRSAYSDKLTAAF
                     QSHLDETHGQIERIDQVVDSEDGLKLKRIKCAAMEGLIEEANEVIESTDKNEVRDAAL
                     IAAAQKVEHYEIASYGTLVTLAEQLGYKKAAKLLKETLEEEKATDVKL"
BASE COUNT          270 a          140 c          181 g          201 t
ORIGIN      
        1 tttcttattt gttactagag ttaattgggt gctgacaagg cacatgttat ttacaatttg
       61 aaaatcagag gagaaatgat atgaccgaac atcgtggcgg tgcaggtaat ttcgctgaag
      121 atcgacaaaa agcttctgac gctggtcgta aaggcggcca acatagtggc ggtaatttta
      181 aaaacgatcc acaaagagca tcagaagcag ggaaaaaagg tggtcagaat agccatggtg
      241 gcggccgtaa atctgactct taatttatca ccccctacat tcgtgaataa ctgaagctaa
      301 ctgcgagcct acgggctcgc agtgaaaact ctggagtgat tatgcaaatt aaatctgttg
      361 aagatatctt catccactta ttatcggata catacagcgc tgagaaacaa ctaacaaagg
      421 cactgtctaa acttagtcgc tctgcatata gtgataaatt aaccgctgct tttcagtcac
      481 atcttgatga gacacatggt caaattgaac gcatcgatca ggttgttgat tctgaagatg
      541 gtcttaaatt gaaaagaatt aagtgtgccg caatggaagg acttatcgaa gaggcgaatg
      601 aggtaattga aagcactgat aaaaatgaag tacgtgatgc tgcattgatt gctgcagcac
      661 aaaaagtaga gcactatgaa attgccagtt atggcacttt ggtaacactc gctgagcaat
      721 taggatataa aaaagcggct aaactcctga aagaaacact ggaagaggaa aaagctactg
      781 atgtcaaatt aa
//
LOCUS       DBFIFI010000508          791 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.514, whole genome shotgun sequence.
ACCESSION   DBFIFI010000508 DBFIFI010000000
VERSION     DBFIFI010000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.514"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..>791
                     /locus_tag="N7B39_005167"
     CDS             <1..>791
                     /locus_tag="N7B39_005167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HGU2424914.1"
                     /translation="ESDPMNVTFDNLAPEVQNAVMVKFDTCENITVDMVISAQELLQE
                     DMATFDGHIVEALMKMPEVNAMYPELKLHAIGWVKHKCKPGAKWPEIQAEMRIWKKRR
                     EGERKEAGKYTSVVDLARARANQQHTENSTGKINPVIAAIHREYKQTWKTLDDELAYA
                     LWPGDVDAGNIDGSIHRWAKNEVIDNGREDWKRISASMRKQPDALRYDRQTIFGLVRE
                     RPIDIHKDPVALNKYITEYLTTKGVFEDEGTNQSATDTLSSPVPE"
BASE COUNT          239 a          194 c          195 g          163 t
ORIGIN      
        1 ctgaatccga tccgatgaac gtcacttttg acaacctggc ccctgaagta cagaacgctg
       61 tcatggttaa gttcgacaca tgtgaaaaca tcactgttga tatggtgatt agcgcacagg
      121 aattgttgca ggaagacatg gcaacattcg acggccatat cgttgaagcg ttgatgaaaa
      181 tgccagaagt taacgccatg tatccggagc ttaagctgca tgccatcggg tgggttaagc
      241 ataaatgtaa gcctggtgcc aaatggcccg aaattcaggc agagatgcgc atctggaaaa
      301 aacgtcgcga aggtgaacgc aaggaagccg gaaaatacac gtctgttgtt gatctcgccc
      361 gcgccagagc caatcaacag cacactgaaa attcaacagg aaaaatcaac ccggtcattg
      421 ctgccattca tcgcgaatac aagcagacat ggaaaacact ggatgacgaa ctggcctacg
      481 ctctctggcc tggtgatgtg gatgccggaa acattgacgg cagcatccat cgctgggcaa
      541 aaaatgaagt tatcgacaac ggccgcgaag actggaagcg tatctcggca tcaatgcgca
      601 aacagcctga tgcccttcgc tacgaccgcc agactatttt tggccttgtc cgtgaacgtc
      661 cgatcgacat tcacaaagac cctgtggcac tgaacaaata cattactgaa tacctgacta
      721 caaagggcgt gtttgaagat gaaggaacaa atcagagcgc aactgatact ctctcgtcgc
      781 cagtaccaga a
//
LOCUS       DBFIFI010000509          788 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.515, whole genome shotgun sequence.
ACCESSION   DBFIFI010000509 DBFIFI010000000
VERSION     DBFIFI010000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.515"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..>788
                     /gene="ehxB"
                     /locus_tag="N7B39_005168"
                     /gene_synonym="hlyB"
     CDS             <1..>788
                     /gene="ehxB"
                     /locus_tag="N7B39_005168"
                     /gene_synonym="hlyB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter
                     permease/ATPase EhxB"
                     /protein_id="HGU2424915.1"
                     /translation="PCYVIWSLFISPLLRRRLDDKFLRNAENQAFLVETVTAINTIKS
                     MAVSPQMIATWDKQLAGYVASSFRVNLVAMTGQQGIQLIQKSVMVISLWMGAHLVISG
                     EISIGQLIAFNMLAGQVIAPVIRLAHLWQDFQQVGISVERLGDVLNTPVEKKSGRNIL
                     PEIQGDIEFKNVRFRYSSDGNVILNNINLYISKGDVIGIVGRSGSGKSTLTKLLQRFY
                     IPETGQILIDGHDLSLADPEWLRRQIGVVLQENILLNRSIIDNI"
BASE COUNT          235 a          126 c          182 g          245 t
ORIGIN      
        1 taccttgtta tgttatatgg tcattgttta tatcaccctt attacgtcga cgtcttgatg
       61 ataagtttct caggaatgca gaaaatcaag cttttcttgt cgaaacggta acagcaataa
      121 atacaatcaa atccatggca gtatcaccac agatgattgc tacatgggat aaacaactgg
      181 ccgggtatgt tgcttccagt ttcagggtaa atctggttgc aatgacaggg cagcagggga
      241 tacagctgat acagaaaagt gtaatggtaa ttagtctatg gatgggagca catcttgtca
      301 tatcgggaga gataagcatc gggcagttaa tcgcgtttaa tatgcttgca ggtcaggtca
      361 ttgctcctgt tatcagactg gctcatcttt ggcaggattt tcagcaggtt ggtatttctg
      421 ttgagcgcct tggtgatgta ttaaatacac cggtagagaa aaagtcaggc agaaatatac
      481 tgccggaaat tcagggggat atcgaattta aaaatgtcag atttcggtat tcttctgacg
      541 gtaatgttat tttgaataat attaatttat acatatcaaa gggggatgtt atcggtatag
      601 ttggccgttc tggttcagga aaaagtacat taacaaaact gcttcagcgc ttttatatac
      661 cagagaccgg acagatttta attgatgggc atgatttatc acttgcagat ccagaatggt
      721 tacgacgcca gattggtgtt gtattgcagg aaaatatact attaaatcgt agtattatcg
      781 ataatatt
//
LOCUS       DBFIFI010000510          785 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.516, whole genome shotgun sequence.
ACCESSION   DBFIFI010000510 DBFIFI010000000
VERSION     DBFIFI010000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..785
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.516"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..591
                     /gene="escU"
                     /locus_tag="N7B39_005169"
     CDS             <1..591
                     /gene="escU"
                     /locus_tag="N7B39_005169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     switch protein EscU"
                     /protein_id="HGU2424916.1"
                     /translation="VLIFYFMGHSYANEFANFTGLNAYQALVVVAFFVFLLWKGVLFG
                     YLLFSVFDFWFQKHEGLKKMKMSKDEVKREAKDTDGNPEIKGERRRLHSEIQSGSLAN
                     NIKKSTVIVKNPTHIAICLYYKLGETPLPLVIETGKDAKALQIIKLAELYDIPVIEDI
                     PLARTLYKNIHKGQYITEDFFEPVAQLIRIAIDLDY"
     gene            complement(588..>785)
                     /gene="etgA"
                     /locus_tag="N7B39_005170"
     CDS             complement(588..>785)
                     /gene="etgA"
                     /locus_tag="N7B39_005170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE muramidase EtgA"
                     /protein_id="HGU2424917.1"
                     /translation="ACINISVAGYILASNIKSRGNTWDAVGAYNAGYFNTPNAVELRR
                     QYAMKIYKTYNKLKNNEQIID"
BASE COUNT          241 a          135 c          145 g          264 t
ORIGIN      
        1 gttctcattt tttattttat ggggcattca tatgcaaatg agtttgctaa tttcacagga
       61 ctgaacgcat atcaagctct tgtcgttgtt gccttttttg tttttctttt atggaaaggc
      121 gtgctattcg gatatctact cttttcagta tttgatttct ggttccagaa gcatgaggga
      181 ctgaagaaaa tgaaaatgag taaagatgag gtgaaacgag aagccaagga tactgatggt
      241 aaccctgaaa ttaaagggga gcgccgtcgc cttcattccg agatacaaag tggaagtttg
      301 gctaataaca tcaaaaaatc aaccgttatt gttaaaaacc cgactcacat tgcgatttgc
      361 ctatactata aacttgggga gactccatta cctttagtta ttgaaacagg aaaagatgcc
      421 aaagctctac agatcattaa actggctgaa ctctatgata ttccagtgat tgaagatatt
      481 cctttagcaa gaactctcta taagaatata cataaaggac aatatataac agaagacttt
      541 tttgaacctg tggcacaatt gattcgtatt gcgatagacc ttgattatta atcgataatt
      601 tgctcattat tctttaattt attatatgtt ttatatattt tcattgcgta ttgccttctg
      661 agttctactg catttggtgt attaaagtaa cctgcattat acgcaccaac tgcatcccat
      721 gtattccctc tggattttat gttagaggct aagatataac cagcaacaga tatgtttata
      781 caggc
//
LOCUS       DBFIFI010000511          780 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.517, whole genome shotgun sequence.
ACCESSION   DBFIFI010000511 DBFIFI010000000
VERSION     DBFIFI010000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.517"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            97..693
                     /gene="fimE"
                     /locus_tag="N7B39_005171"
     CDS             97..693
                     /gene="fimE"
                     /locus_tag="N7B39_005171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313299.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HGU2424918.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTLERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
BASE COUNT          218 a          147 c          184 g          231 t
ORIGIN      
        1 gtgtggttat ctttttatct attgggctaa ttttgaccga ttgaggtttc ctataggtat
       61 tcattcaaat atatctcagt taggagtact actattgtga gtaaacgtcg ttatcttacc
      121 ggtaaagaag ttcaggccat gatgcaggcg gtttgttacg gggcaacggg agccagagat
      181 tattgtctta ttctgttggc atatcggcat gggatgcgta ttagtgaact gcttgatctg
      241 cattatcagg atcttgacct taatgaaggc cgaataaata ttcgccgact gaagaacgga
      301 ttttctaccg ttcacccgtt acgttttgat gagcgtgaag ctgtggaacg ctggacccta
      361 gaacgtgcta actggaaagg cgctgaccgg accgacgcca tatttatttc tcgccgcggg
      421 agtcggcttt ctcgccagca ggcctatcgc attattcgtg atgccggtat tgaagctgga
      481 accgtaacgc agactcatcc tcatatgtta aggcatgctt gcggttatga actggcggag
      541 cgtggtgcag atactcgttt aattcaggat tatctcgggc atcgaaatat tcgccatact
      601 gtacgttata ccgccagtaa tgctgctcgt tttgccggat tatgggaaag aaataatctc
      661 ataaacgaaa aattaaaaag agaagaagtt tgatttaact tattgataat aaagttaaaa
      721 aacaaataaa tacaagacaa ttggggccaa actgtccata tcataaataa gttacgtatt
//
LOCUS       DBFIFI010000512          770 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.518, whole genome shotgun sequence.
ACCESSION   DBFIFI010000512 DBFIFI010000000
VERSION     DBFIFI010000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..770
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.518"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            67..>770
                     /gene="nleL"
                     /locus_tag="N7B39_005172"
     CDS             67..>770
                     /gene="nleL"
                     /locus_tag="N7B39_005172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector E3 ubiquitin
                     ligase NleL"
                     /protein_id="HGU2424919.1"
                     /translation="MLPTTNISVNSGVISFESPVDSPSNEDVEVALEKWCAEGEFSEN
                     RHEVASKILDVISTNGETLSISEPITTLPDLLPGSLKELVLNGCTELKSINCLPPNLS
                     SLSMVGCSSLEVINCSIPENVINLSLCHCSSLKHIEGSFPEALRNSVYLNGCNSLNES
                     QCQFLAYDVSQGRACLSKAELTADLIWLSANRTGEESAEELNYSGCDLSGLSLVGLNL
                     SSVNFSGAVLDDTDLR"
BASE COUNT          229 a          132 c          162 g          247 t
ORIGIN      
        1 aagttgtccc ggcagttgtg ttttctaatt ttactttagt ggactaagta aaaaggagtg
       61 agataaatgc tgcccactac aaatatctct gtaaattctg gagtaatatc ttttgaaagt
      121 cctgtagatt caccatctaa cgaggatgtt gaagttgccc tcgaaaagtg gtgtgctgag
      181 ggagaattta gcgaaaatcg tcatgaggtt gcatcaaaaa tacttgatgt tataagtact
      241 aatggagaga ctttatcaat cagtgagcca ataacaacat taccagactt gcttccaggt
      301 tctctgaaag aactggtttt gaatggatgt acagagctta aatcaataaa ctgcttaccc
      361 cccaacttat cttcattaag tatggttgga tgctcatcat tagaggttat aaattgcagc
      421 atacctgaaa atgtcattaa tttatcttta tgccattgta gttctttgaa acatatagaa
      481 ggttcctttc ctgaggcact cagaaattcc gtatatttaa atggctgtaa ttcattaaat
      541 gaatcgcaat gtcaattcct tgcatatgat gtcagtcaag gccgtgcctg cctgagcaaa
      601 gctgagctta ctgctgactt aatttggttg tcagctaacc gaacgggtga agagtctgct
      661 gaagaattga attactctgg atgtgacttg tcaggtctaa gtcttgtagg gctgaattta
      721 tcatcagtaa atttttctgg agcagtgctt gatgatacag atctcaggat
//
LOCUS       DBFIFI010000513          769 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.519, whole genome shotgun sequence.
ACCESSION   DBFIFI010000513 DBFIFI010000000
VERSION     DBFIFI010000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..769
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.519"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            103..609
                     /gene="yciE"
                     /locus_tag="N7B39_005173"
     CDS             103..609
                     /gene="yciE"
                     /locus_tag="N7B39_005173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HGU2424920.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(669..>769)
                     /locus_tag="N7B39_005174"
     CDS             complement(669..>769)
                     /locus_tag="N7B39_005174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HGU2424921.1"
                     /translation="DTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
BASE COUNT          242 a          157 c          156 g          214 t
ORIGIN      
        1 aaactgactg atctggccct taataacgta aataagaaag ccgaaaataa agcctgaaat
       61 atgaacttta acttttggtc attttataaa gaggacattt tcatgaatcg tattgaacat
      121 tatcatgact ggttacgtga cgcccatgca atggaaaagc aagccgaatc tatgcttgaa
      181 tccatggcca gccgtataga taattatcct gaactacgtg ctcgtattga acaacatctt
      241 agtgaaacca aaaaccagat tgttcaactg gaaactattc ttgatcgtaa tgacatttca
      301 cgttcagtca ttaaagattc catgagtaaa atggctgcgc ttgggcagtc aatcggtggt
      361 atattccctt ctgatgaaat agtcaaaggc tctattagcg gatatgtctt cgagcaattt
      421 gaaatcgcct gttacacctc tctattagca gcagcaaaaa atgccggtga tacagcctca
      481 attccgatca tcgaagcgat tttaaatgag gaaaagcaaa tggccgactg gctgattcag
      541 catattccgc aaacaactga gaaattttta attcgctctg aaactgatgg cgtagaagcg
      601 aagaaataat accctttatg ccatgtcctt attgaccccg tatattacgg ggtcgttttt
      661 gtgcggaatt aaaaacgata tcctgctgag aacataaaca cccacggatc gaggcgtacg
      721 ctatcatgtt gctgtgcacc gcccagttta taattggcgg tggtatcga
//
LOCUS       DBFIFI010000514          767 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.520, whole genome shotgun sequence.
ACCESSION   DBFIFI010000514 DBFIFI010000000
VERSION     DBFIFI010000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.520"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..>767
                     /locus_tag="N7B39_005175"
     CDS             <1..>767
                     /locus_tag="N7B39_005175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS domain-containing protein"
                     /protein_id="HGU2424922.1"
                     /translation="TDLIPEGVIRTDDERTHRYHYDSQHRLVHYTRTQYEEPLVESRY
                     LYDPLGRRVAKRVWRRERDLTGWMSLSRKPQVTWYGWDGDRLTTIQNDRTRIQTIYQP
                     GSFTPLIRVETATGELAKTQRRSLADALQQSGGEDGGSVVFPPVLVQMLDRLESEILA
                     DRVSEESRRWLASCGLTVAQMQSQMDPVYTPARKIHLYHCDHRGLPLALISKEGATEW
                     CAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEESGLY"
BASE COUNT          175 a          219 c          252 g          121 t
ORIGIN      
        1 aaccgacctc atcccggaag gggttatccg cacggatgat gagcggactc accggtacca
       61 ttacgacagt cagcaccggc tggtgcacta cacgcggaca caatatgaag agccgctggt
      121 cgaaagccgc tatctttacg acccgctggg ccgcagggtg gcaaaacggg tgtggcgacg
      181 tgaacgggac ctgacgggct ggatgtcgct gtcacggaaa ccgcaagtga cctggtacgg
      241 ctgggacggc gaccggctga ccacaataca gaacgacaga acccgcatcc agacgattta
      301 tcagccgggg agcttcacgc cactcatcag ggttgaaacc gccaccggtg agctggcgaa
      361 aacgcagcgc cgcagcctgg cggatgcgct tcagcagtcc ggcggcgaag acggtggcag
      421 tgtggtgttc ccgccggtgc tggtgcagat gctcgaccgg ctggaaagtg aaatcctggc
      481 tgaccgggtg agtgaggaaa gccgccgctg gctggcatcg tgcggcctga ctgtggcgca
      541 gatgcaaagc cagatggacc cggtatacac gccggcgcga aaaatccacc tgtaccactg
      601 cgaccatcgc ggcctgccgc tggcccttat cagtaaggaa ggggcaacag aatggtgcgc
      661 agaatacgat gagtggggca acctgctgaa tgaagagaac ccgcatcagc tgcagcagct
      721 tatccgcctg ccggggcagc agtatgatga ggagtccggc ctgtatt
//
LOCUS       DBFIFI010000515          759 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.521, whole genome shotgun sequence.
ACCESSION   DBFIFI010000515 DBFIFI010000000
VERSION     DBFIFI010000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.521"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            12..728
                     /gene="yeeN"
                     /locus_tag="N7B39_005176"
     CDS             12..728
                     /gene="yeeN"
                     /locus_tag="N7B39_005176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGU2424923.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGISEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          250 a          134 c          163 g          212 t
ORIGIN      
        1 agatcattcc tgtgggacgt aaatgggcca atattgttgc taaaaaaacg gctaaagacg
       61 gtgcaacgtc taaaatttat gcaaaattcg gtgtagaaat ctatgctgcc gctaaacaag
      121 gtgaacccga tccagaatta aacacatctt taaaattcgt tattgaacgt gcaaagcagg
      181 cacaagttcc aaagcacgtt attgataaag caattgataa agccaaaggc ggcggagatg
      241 aaacattcgt gcagggccgt tatgaaggct ttggtcctaa tggctcaatg attatcgccg
      301 agacattgac ttcaaatgtt aaccgtacga ttgctaacgt tcgcacaatt ttcaataaaa
      361 aaggcggcaa tatcggagcg gcaggttctg tcagctatat gtttgacaat actggtgtga
      421 ttgtatttaa aggatcagac cctgaccata tttttgaaat tttacttgaa gctgaagttg
      481 atgttcgtga tgtgactgaa gaagaaggta acattgttat ttataccgaa cctactgacc
      541 ttcataaagg aatcgcggct ctaaaagcag ctggaatcag tgagttctca acaacagaat
      601 tagaaatgat tgctcaatct gaagttgagc tttcaccaga agatttagaa atctttgaag
      661 ggcttgttga tgcccttgaa gatgacgacg atgtacaaaa agtttatcat aacgtcgcaa
      721 atctctaatt atattttaaa gaaatctgtc tttacggca
//
LOCUS       DBFIFI010000516          752 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.522, whole genome shotgun sequence.
ACCESSION   DBFIFI010000516 DBFIFI010000000
VERSION     DBFIFI010000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.522"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..615
                     /locus_tag="N7B39_005177"
     CDS             <1..615
                     /locus_tag="N7B39_005177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HGU2424924.1"
                     /translation="GQLEAWQENAMFDTGRYRRKKIRYRDEVTGKMITRDNPPIPGKQ
                     SLAKVTSIPLVSPVEFSTSSWRRAVLSLEEHHKAWLLWCYSGSICWEYQIAITQWAWN
                     EFNTQSGTRKIAGKTQERLKKLIWLAAQAVKAELFGGEGYEYKELALLVGVTTKNWSK
                     TFTRHWVAMKHIFHRLDSEALLFVMRTRSKQKAAFSKQSVAKVD"
BASE COUNT          225 a          138 c          205 g          184 t
ORIGIN      
        1 ggtcagcttg aagcctggca ggagaatgcc atgttcgata cagggcgtta caggcgaaaa
       61 aaaatccggt accgcgatga agtgactgga aaaatgataa cgcgggataa tccaccaatc
      121 ccgggaaagc aatcgctggc gaaggtgacg tcaattcctc tggtcagtcc ggttgagttt
      181 tcgacatcat cgtggcggcg ggctgttctg tctcttgaag aacatcataa agcctggttg
      241 ttgtggtgtt acagcgggag tatttgttgg gaatatcaga tcgcgataac acagtgggcg
      301 tggaatgaat ttaatactca atccggtacc agaaaaattg cagggaaaac gcaggaacgc
      361 ctgaaaaaat taatctggct ggcggcgcag gcagtaaaag cagaactttt tggtggggaa
      421 ggttatgaat acaaggagct ggcattactg gtgggagtga caactaaaaa ctggtccaaa
      481 acatttactc gtcactgggt tgcaatgaaa cacatttttc accgactgga tagtgaggct
      541 ttattgtttg taatgagaac gcgttcaaaa caaaaggcgg cattttcaaa gcaaagtgtt
      601 gcaaaagtag attgaaaggc atatatttca tgcaaatctg atattttgcc gattttgtac
      661 gtgatggcaa aagcaaacaa aacccgccca caagcgggtt tttttgtgcc acttatctcg
      721 gatagacatg gtgaatgcgc tggtggagga ag
//
LOCUS       DBFIFI010000517          751 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.523, whole genome shotgun sequence.
ACCESSION   DBFIFI010000517 DBFIFI010000000
VERSION     DBFIFI010000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.523"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(72..674)
                     /gene="lpfA2"
                     /locus_tag="N7B39_005178"
     CDS             complement(72..674)
                     /gene="lpfA2"
                     /locus_tag="N7B39_005178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA2"
                     /protein_id="HGU2424925.1"
                     /translation="MKPNMIVGALALTSVFMAGHLQAADGAVHFRGEIIDSTCEVTPE
                     TKDQVVDLGKVNRTAFSGVDDVAAPTAFSIDLTQCPETFKSAAIRFDGNEDAHGNGNL
                     AIGTPLDNSNDAAAGISPSDNSGDYTGAGAVSAAKGVAIRLYNRADNTQVKLYENSAS
                     TPISNGNASMKFMARYIATETTIDPGTANADSQFTVEYIK"
BASE COUNT          217 a          165 c          149 g          220 t
ORIGIN      
        1 cccttcattt aagtaaaaga gaaccataat ggcgagcgcc attttattta aggtattatg
       61 ttaagaatga tttattttat atattcaact gtaaactgcg agtcggcgtt agctgtacca
      121 gggtcaatag tcgtttccgt agcaatataa cgagccatga acttcatgga tgcattacca
      181 ttagaaatcg gagttgatgc agaattttca tataacttga cctgagtgtt atctgcacgg
      241 ttatataaac gaatagctac gcctttcgct gcactaacgg cacccgcacc agtataatcc
      301 ccactgttat cactcgggct aataccagcg gcagcatcgt tagagttatc cagcggggta
      361 ccaattgcca ggttgccatt accatgagca tcttcattac catcgaaacg aattgcggcg
      421 gacttaaagg tttccgggca ttgagtcaga tcgatagaaa aagccgtcgg ggcagccaca
      481 tcatcgacgc cactaaaggc tgtacggttt actttgccta aatcaacgac ctgatcttta
      541 gtttcaggag tgacttcgca agtactgtca ataatttcac cacggaaatg gactgctcca
      601 tcagccgcct gtaggtgacc tgccataaac acagaagtta acgctaatgc tcctacaatc
      661 atatttggtt tcataaatat aactccatta atttgatatt attcctgtgt gttatccatt
      721 tattaaattt ggataaacct gatcggtatt t
//
LOCUS       DBFIFI010000518          746 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.524, whole genome shotgun sequence.
ACCESSION   DBFIFI010000518 DBFIFI010000000
VERSION     DBFIFI010000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.524"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
BASE COUNT          261 a          129 c          137 g          219 t
ORIGIN      
        1 aaatagataa tgcaatccgg gatctttcct ctgaaacaga gaggttcaag ataaagtatc
       61 aacaagagaa tacacttaat gatgtagttc aaaatatcag gacttggcta aaagaaaatc
      121 aaaatatggt gaaagctatt aagaaaattg atactgaata acatggcgaa taaataaacg
      181 gatcaagtcg ttcctatgac aatgaggtta tacgaaaaga cgcttactca tcagaaatct
      241 ggtatgcttt aattaaacca gtgtcgctga tgtgtaagcg ttgacgttgg gagacgatta
      301 aatggttata ccactcctac gtcttttcca tacggcgtat aagagtgcaa catgttgagt
      361 tttccgagtg aatggtgcat tttactaacc gagccatttg aatcaaccaa cgcaataaaa
      421 aagactggaa taataagcct gtatttatta tttatcgcaa tcgcacttta cggttttatc
      481 caactgaaaa gagactgctt ccagcggttg tggattatcc ctgggcgata gtcatagtct
      541 ggtgtcagga ttcacaaatg acctgtttcc ttacatggat ataaacacaa catagagtgc
      601 ggtagttaat ctgaataatc taatttcggc tattcagcac aaaacaacat tattgattct
      661 ttataagaga gaatctctcc atcattttta tataaatcat tattaagaaa atccagaagc
      721 tcttgcctta cactataaat attgac
//
LOCUS       DBFIFI010000519          739 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.525, whole genome shotgun sequence.
ACCESSION   DBFIFI010000519 DBFIFI010000000
VERSION     DBFIFI010000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.525"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(118..>739)
                     /locus_tag="N7B39_005179"
     CDS             complement(118..>739)
                     /locus_tag="N7B39_005179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HGU2424926.1"
                     /translation="NSPAQLLKITKSGDIMDRAPLPFISDAETIEHIQGNIFAAVDEK
                     TSELFFFTVTKDMHISFRNKIQLEKFNKKNRGFEGLAWKADDRMLFVAKERRPSKIFI
                     YQLSPDLLSVKQATIPEALNDIRVNDISGLAFNNESLMILSDESRKLLKFNLTEMSFI
                     EMLDLTKGNHSLTSDLPQPEGIVTLPDESIYVASEPDILAKFVPNK"
BASE COUNT          206 a          135 c          143 g          255 t
ORIGIN      
        1 taactgatat ccttttcaac tattaatgtg tttatttagc gtgaaattat actcgtattt
       61 ttataacttt atgagattgc caggtttggt cgataaataa taaggccagt gacagactta
      121 cttattaggg acaaatttcg cgaggatatc aggttcactg gcaacataaa tgctttcatc
      181 aggcagagtt acaattcctt caggttgggg caaatcactg gtcagagaat gatttccttt
      241 tgtcaggtca agcatctcaa tgaaagacat ctctgtcagg ttaaacttga gtaatttacg
      301 cgattcatcg gacagaatca tcagactttc attattaaag gccaggccgg atatatcgtt
      361 aacacgtata tcatttagtg cctctggaat agttgcttgc tttacactca gcaggtctgg
      421 agaaagttga tatataaata ttttacttgg gcgtctttct tttgcgacaa acagcatccg
      481 atcatctgct ttccatgcca gcccttcaaa ccctctattc tttttattga atttttctaa
      541 ctgaatttta tttcgaaagg agatatgcat atcttttgta actgtaaaaa agaatagttc
      601 agaagtcttt tcgtctactg cagcaaaaat gttcccttgt atgtgttcaa ttgtttcggc
      661 atcagagata aaaggcagtg gggctctgtc catgatgtcg ccacttttgg ttattttaag
      721 tagctgtgca gggctattc
//
LOCUS       DBFIFI010000520          730 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.526, whole genome shotgun sequence.
ACCESSION   DBFIFI010000520 DBFIFI010000000
VERSION     DBFIFI010000520.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..730
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.526"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            504..692
                     /locus_tag="N7B39_005180"
                     /pseudo
     CDS             504..692
                     /locus_tag="N7B39_005180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          216 a          141 c          155 g          218 t
ORIGIN      
        1 actgatgtac aaaacacaac aatagcttca tgatatataa atacgtgatg acgagtggta
       61 ctccgcatgg ttatttaact tctaagcacc actaatgatc ctgagcaact tcgtatttgg
      121 gccattacca tggagtagaa agctgtgtct gagaatgcag aatttgagaa aagaattcgc
      181 tttcttggag aaattctgaa aatggcacgc taacaacgtt ttgttaaaaa atgtgaatta
      241 cttgccgggg ctgcattttc tattcgagga gaatgttgac gtagatatta cagaaatctc
      301 agcgcatcag gataaactgc taccttaggt caaccaccaa aagaaaatac aatacgtcca
      361 gtttgtatgc ctttaataac acatcttcta aatgttgaag aattatcccg cctgctgaag
      421 aacgttgctc tgagtgtaat aaaccttttt attcttgatg atgtcagcga gaagatggaa
      481 tatatccccg actgggtcgt ggttacccgg ttaaacatct ggtggtgcta ataaatctgg
      541 caagtcacat tctgttgaat gctgtgacag tgccttaccg agagagcgaa attgtggtgt
      601 tctagtgcat agtcgccacc attccggata attctattac gttgttttac aagttctttt
      661 acagcgcaga tctgtcgctg acgttgaatc aatagcctgg atttcagaac acaatgcccg
      721 tttgctggtt
//
LOCUS       DBFIFI010000521          728 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.527, whole genome shotgun sequence.
ACCESSION   DBFIFI010000521 DBFIFI010000000
VERSION     DBFIFI010000521.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.527"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..>728
                     /gene="rfaL"
                     /locus_tag="N7B39_005181"
     CDS             <1..>728
                     /gene="rfaL"
                     /locus_tag="N7B39_005181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HGU2424927.1"
                     /translation="LRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFA
                     DYRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLV
                     IGLIWILMFKQWKLLLVGVMVAIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQG
                     SALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLG
                     LVSLMMLYCAILKECIKNGVKNKY"
BASE COUNT          217 a          116 c          145 g          250 t
ORIGIN      
        1 tattgagaga cgagaaaaga gaagatgtcg aaaaaatcgt tttcttctca tttattagtg
       61 cgttgggctt acgctgtttt tctgaattga ttacctatta taaggactat caacaaggga
      121 taatgccatt cgcagattat agacaccgta gcatttctga ctcgatggtc tttttattcc
      181 ctgcattgtt aaatctctgg cttatcaaat cagcaaaata ccgcatttct tttgtggttc
      241 taagcgttat ttttattttt ctgatattag gaactttatc cagaggggcc tggctttccg
      301 tgttagtcat tggattaata tggattctga tgtttaaaca atggaagtta ctattagtag
      361 gagtaatggt tgccatcatt gcattgtcgg ttattttcac acataaggag atgaccgcaa
      421 agctaacgta taaacttcaa caaactaata gttcttatcg ctatgcaaat ggtactcaag
      481 gcagcgcact cgatctaata ttagaaaatc ctgttattgg ttatggttac ggtaacgttg
      541 catataaaga tgtctataat aaacgtgtca ttgattatcc agaatggacc tttagacaat
      601 caatagggcc acataatttt gcgctattca tctggtttgg cactggttta ttagggctgg
      661 taagtcttat gatgctatac tgtgcaatat tgaaagagtg tataaaaaat ggcgtcaaga
      721 ataaatat
//
LOCUS       DBFIFI010000522          724 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.528, whole genome shotgun sequence.
ACCESSION   DBFIFI010000522 DBFIFI010000000
VERSION     DBFIFI010000522.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..724
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.528"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..>724)
                     /locus_tag="N7B39_005182"
     CDS             complement(<1..>724)
                     /locus_tag="N7B39_005182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HGU2424928.1"
                     /translation="RRMAKRVWRRERDLTGWMSLSRKPEVTWYGWDGDRLTTVQTDTT
                     RIQTVYEPGSFTPLIRVETENGEREKAQRRSLAETLQQEGSENGHGVVFPAELVRLLD
                     RLEEEIRADRVSSESRAWLAQCGLTVEQLARQVEPEYTPARKVHFYHCDHRGLPLALI
                     SEDGNTAWRGEYDEWGNQLNEENPYYLHQPYRLPGQQHDEESGLYYNRNRYYDPLQGR
                     YITQDPIGLAGGWNLYNYPLNPI"
BASE COUNT          109 a          258 c          183 g          174 t
ORIGIN      
        1 ttatcggatt cagtgggtaa ttatacagat tccatccccc cgccagccca atggggtctt
       61 gtgtaatata cctcccctgt agcgggtcat agtaccggtt ccggttatag tacagccctg
      121 attcctcatc atgctgctgc cccggcagac ggtatggctg gtgcaggtaa tacgggttct
      181 cttcattaag ctggttgccc cattcatcat actccccgcg ccacgccgta ttgccgtctt
      241 cgctgatgag cgccagcggc aggccccggt ggtcgcagtg gtaaaaatga acttttcgcg
      301 ccggtgtgta ttccggctcc acctgtctgg ccagttgctc caccgtcagc ccgcactgcg
      361 caagccacgc ccggctttca ctgctcacgc ggtctgcccg gatttcttcc tccagcctgt
      421 ccagcagccg caccagttca gccgggaaca ccacgccgtg gccgttctca ctcccttcct
      481 gctggagcgt ctctgccagg ctgcgccgct gcgctttttc ccgctcgccg ttctctgtct
      541 cgacccggat gagcggcgtg aagcttcccg gctcgtatac cgtctggata cgtgtggtgt
      601 cagtctgcac cgtcgtcagc ctgtctccgt cccagccata ccacgtcacc tccggtttac
      661 gcgacagcga catccacccc gtcaggtcac gctcccgccg ccagacccgt tttgccattc
      721 gccg
//
LOCUS       DBFIFI010000523          721 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.529, whole genome shotgun sequence.
ACCESSION   DBFIFI010000523 DBFIFI010000000
VERSION     DBFIFI010000523.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..721
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.529"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..>721
                     /locus_tag="N7B39_005183"
     rRNA            <1..>721
                     /locus_tag="N7B39_005183"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          182 a          166 c          236 g          137 t
ORIGIN      
        1 gtaacggctc acctaggcga cgatccctag ctggtctgag aggatgacca gccacactgg
       61 aactgagaca cggtccagac tcctacggga ggcagcagtg gggaatattg cacaatgggc
      121 gcaagcctga tgcagccatg ccgcgtgtat gaagaaggcc ttcgggttgt aaagtacttt
      181 cagcggggag gaagggagta aagttaatac ctttgctcat tgacgttacc cgcagaagaa
      241 gcaccggcta actccgtgcc agcagccgcg gtaatacgga gggtgcaagc gttaatcgga
      301 attactgggc gtaaagcgca cgcaggcggt ttgttaagtc agatgtgaaa tccccgggct
      361 caacctggga actgcatctg atactggcaa gcttgagtct cgtagagggg ggtagaattc
      421 caggtgtagc ggtgaaatgc gtagagatct ggaggaatac cggtggcgaa ggcggccccc
      481 tggacgaaga ctgacgctca ggtgcgaaag cgtggggagc aaacaggatt agataccctg
      541 gtagtccacg ccgtaaacga tgtcgacttg gaggttgtgc ccttgaggcg tggcttccgg
      601 agctaacgcg ttaagtcgac cgcctgggga gtacggccgc aaggttaaaa ctcaaatgaa
      661 ttgacggggg cccgcacaag cggtggagca tgtggtttaa ttcgatgcaa cgcgaagaac
      721 c
//
LOCUS       DBFIFI010000524          711 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.530, whole genome shotgun sequence.
ACCESSION   DBFIFI010000524 DBFIFI010000000
VERSION     DBFIFI010000524.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..711
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.530"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..582)
                     /locus_tag="N7B39_005184"
     CDS             complement(<1..582)
                     /locus_tag="N7B39_005184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HGU2424929.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEIFALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHTCIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDHFGQIHYLLLEPEEGKIFTKGDKVLIICRLS"
BASE COUNT          231 a          127 c          157 g          196 t
ORIGIN      
        1 agaaagtcgg caaataatta acactttgtc tcctttagtg aaaatttttc cttcttcagg
       61 ctccagtagt aaataatgta tttggccaaa atgatcggtg agttttcctt cgcagggatt
      121 acctgaagtg gcttgatgac cggtaattaa cgccatgcta ccaatatatt cttcttctgt
      181 aatagctgag ctatgatcgc gtggaatcca gggcgcaact attttaccgg tataatgcac
      241 cgcaataatc gtaaatagta aactgacagg aaccacaaac aggttcgaga gtggtgattg
      301 ccagaccatg atgcaggtat gctgcaacag gatgccaata agaccaaaaa aaccagccag
      361 taagcaaaga acgacgagag ctggcaatct tccgatattg agataatgaa gtgcctggct
      421 aatatgacct gtggttatgg aatcataatg gtcaagatgt gcatcaagag caccggagag
      481 catgtgaccg cagataagcg caaaaatttc taaaaggccg atcaacagta caaaggaaat
      541 agcgaaaaga taaggggtgt tatagtcggc gaataaaatc atatgtcact ccaatgtcca
      601 tttagtgacg caatatttac tttgtgatag tttccttgct tacatttgtc tttgttaata
      661 ataacatatg ttagagaggt tctggaagag aaagtctttt aatatggttt t
//
LOCUS       DBFIFI010000525          707 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.531, whole genome shotgun sequence.
ACCESSION   DBFIFI010000525 DBFIFI010000000
VERSION     DBFIFI010000525.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.531"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..444)
                     /locus_tag="N7B39_005185"
     CDS             complement(<1..444)
                     /locus_tag="N7B39_005185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HGU2424930.1"
                     /translation="MPLTSDIRSHSFNLGVEVVRARIVANGRGDITVGGETVSIVYDS
                     TNGRFSSSGGNGGLLSELLLLGFNSGPRALGERMLSMLSDSGEAQSQESIQNKISQCK
                     FSVCPERLQCPLEAIQCPITLEQPEKGIFVKNSDGSDVCTLFDAAA"
     gene            complement(558..>707)
                     /locus_tag="N7B39_005186"
     CDS             complement(558..>707)
                     /locus_tag="N7B39_005186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HGU2424931.1"
                     /translation="DENGIIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGTWEN
                     IGGMK"
BASE COUNT          209 a          195 c          120 g          183 t
ORIGIN      
        1 tgcagcggca tcaaataaag tacatacatc tgaaccatct gaattcttca caaaaatacc
       61 tttttcaggc tgctccagtg taattggaca ctgaatagcc tcaagcgggc actgaagtct
      121 ctctggacaa acagaaaact tacattgaga tattttgttc tgaatactct cttgcgattg
      181 tgcttcacct gagtccgaaa gcatacttag cattctctca ccaagggctc gaggaccact
      241 attaaatccc aaaagcaata actcagaaag caatccgcca ttaccgccac tggatgaaaa
      301 gcgcccatta gtagaatcat acacaatact gacagtttca ccaccgactg taatatctcc
      361 gcgcccattg gctacaattc gggcacgaac aacctccacc ccaagattaa atgaatgtga
      421 tctaatatct gaggttaatg gcataacaaa gctccctatt ttaatttgaa ctccagactt
      481 aaatagctgt aacaaacatc ccccatacgt gacaacaaaa aaccggagcc ggactccggt
      541 ttttgtgaag ctgtcggcta tttcatcccg ccaatatttt cccacgtccc gtcagcacgc
      601 aggatttgca gcggtcttac cacacactgt atctgctttt tatccgcatc cagtatcacc
      661 acctgcgtga ttaccctgtc ctgctccggg ataataccat tctcatc
//
LOCUS       DBFIFI010000526          706 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.532, whole genome shotgun sequence.
ACCESSION   DBFIFI010000526 DBFIFI010000000
VERSION     DBFIFI010000526.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.532"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            10..561
                     /gene="ybcL"
                     /locus_tag="N7B39_005187"
     CDS             10..561
                     /gene="ybcL"
                     /locus_tag="N7B39_005187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HGU2424932.1"
                     /translation="MKTLIVSTVLAFITFSAQAAAFQVASNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGVPEGTKSFAVTVYDPDAPTGSGWWHWTVVNIPATVTYLPVDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTEKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            571..>706
                     /locus_tag="N7B39_005188"
     CDS             571..>706
                     /locus_tag="N7B39_005188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HGU2424933.1"
                     /translation="MLARGKTNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKKVINW
                     E"
BASE COUNT          209 a          127 c          171 g          199 t
ORIGIN      
        1 gaggttttta tgaaaacact tatcgtttca actgtattgg cattcataac attttctgcg
       61 caggctgcag catttcaggt cgctagtaat gaaataaaaa caggagagca acttacaacg
      121 tctcatgtct tttctggatt tgggtgtgaa ggtggtaata catcgccctc attaacctgg
      181 tctggtgttc ctgaaggtac caaaagcttt gccgtaactg tatatgatcc agatgcacct
      241 acaggcagtg gttggtggca ttggactgtt gttaatattc cagcaacagt aacatatttg
      301 cccgttgatg cagggagacg tgatggaaca aaactgccga ctggtgctgt tcaaggccga
      361 aatgattttg gctatgctgg gtttggtggc gcatgtcctc ctaaaggaga taaaccacat
      421 cattaccagt ttaaagtatg ggctctaaaa actgaaaaga ttcctgtaga ttctaactcc
      481 agcggagcgt tagttggtta tatgcttaat gctaataaaa tcgcaaccgc tgagataaca
      541 ccagtttatg agataaagta gggtgagagt atgctggcaa gaggtaagac taacttaaag
      601 atcgaagaaa tacggatgca taaacatcat gagattcata gggttaagcc tcttatgcca
      661 gctttgtgtc gtatccgtca gggaaagaaa gttatcaatt gggaga
//
LOCUS       DBFIFI010000527          706 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.533, whole genome shotgun sequence.
ACCESSION   DBFIFI010000527 DBFIFI010000000
VERSION     DBFIFI010000527.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.533"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            85..510
                     /locus_tag="N7B39_005189"
     CDS             85..510
                     /locus_tag="N7B39_005189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU2424934.1"
                     /translation="MELQDWRKEPRKNYSNEFKLRMVELASQPGASVARIAREHDIND
                     NLLFKWLRLWQNEGRISRRLQVTTSSDTGVELLPVEITPDEQKEPVAAIAPSLSTSTQ
                     TSVSAGSCKVEFRHGNMTLENPSPELLTLLIRELTGRGR"
     gene            507..581
                     /locus_tag="N7B39_005190"
                     /pseudo
     CDS             507..581
                     /locus_tag="N7B39_005190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          203 a          167 c          167 g          169 t
ORIGIN      
        1 taagcgtaaa ctgaccgccg tatgtagcca tcagacgaaa attggtaact tagacgccca
       61 tctgatatag acggacatct aagtatggaa ttacaggact ggcgaaaaga acctcgtaaa
      121 aactattcga atgaattcaa acttcgtatg gtggaactgg catcacaacc aggagcctct
      181 gttgcccgta ttgcccggga acacgatatc aatgataacc tcctgttcaa atggctcagg
      241 ctctggcaga acgaagggcg tatatcacgg cgtcttcagg taacgacctc ttctgacact
      301 ggcgttgaat tattacctgt agaaataacg ccggatgagc agaaagaacc ggtggcggcc
      361 attgcgccgt ctttatccac ttccactcag accagtgtca gtgccggctc ctgcaaggtg
      421 gagttccgtc acggtaacat gacgctggaa aatccatcgc cagagctgct cacgctgttg
      481 atccgtgaac tgaccgggag gggacgatga tctcactccc gtcaggtacc cgcatctggc
      541 tcgttgccga cgttaccgat atgcgtaaat ccttcaacgg atacgacata tgctgaagga
      601 ggagcagtaa tggattaagt gataacaaat acccagaaat agaggttcaa atccagctta
      661 gttagtacat aaatactatt ttgatgaaaa ttattatatg tttcct
//
LOCUS       DBFIFI010000528          705 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.534, whole genome shotgun sequence.
ACCESSION   DBFIFI010000528 DBFIFI010000000
VERSION     DBFIFI010000528.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..705
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.534"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..>705
                     /locus_tag="N7B39_005191"
     CDS             <1..>705
                     /locus_tag="N7B39_005191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HGU2424935.1"
                     /translation="AIAYMRFSSPGQMSGDSLNRQRRLIAEWLKVNSDYYLDTITYED
                     LGLSAFKGKHAQSGAFSEFLDAIEHGYILPGTTLLVESLDRLSREKVGEAIERLKLIL
                     NHGIDVITLCDNTVYNIDSLNEPYSLIKAILIAQRANEESEIKSSRVKLSWKKKRQDA
                     LESGTIMTASCPRWLSLDDKRTAFVPDPDRVKTIELIFKLRMERRSLNAIAKYLNDHA
                     VKNFSGKESAWGPSVI"
BASE COUNT          241 a          110 c          157 g          197 t
ORIGIN      
        1 aagccatagc atatatgcga ttttcatcac caggtcagat gtctggcgac tcattaaacc
       61 gacagagaag acttattgct gaatggttaa aggtaaatag tgattattat cttgatacca
      121 taacatatga agatttagga ttaagtgcat tcaaaggaaa gcatgcacaa tcaggagctt
      181 tttcggaatt tttagatgct atagagcatg gttatatatt gccaggaact acattgttag
      241 ttgaaagtct ggacagactt tcaagagaaa aagtcggtga agcgattgaa cgtctgaaat
      301 tgattttgaa tcacggtatt gatgttataa ctctttgcga caatacagtc tataatattg
      361 actctttgaa tgagccatat tcattaataa aagccatact tatagcacaa agggcaaatg
      421 aagaaagcga gataaagtca agtcgggtta aattatcatg gaagaaaaaa cggcaggatg
      481 cactggaatc aggtacgatt atgacggcgt cttgtccgag atggctctcc ttagatgaca
      541 aaagaacggc ttttgttcca gaccccgaca gggtgaaaac tattgagcta atttttaaac
      601 tcaggatgga aaggcgctca ttgaatgcaa tagccaagta tttaaatgat catgctgtaa
      661 agaatttctc aggaaaagaa agtgcatggg ggccttctgt aattg
//
LOCUS       DBFIFI010000529          696 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.535, whole genome shotgun sequence.
ACCESSION   DBFIFI010000529 DBFIFI010000000
VERSION     DBFIFI010000529.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.535"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            94..483
                     /locus_tag="N7B39_005192"
     CDS             94..483
                     /locus_tag="N7B39_005192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HGU2424936.1"
                     /translation="MQKSNKVIISCGMALYFLCGGITVAEELDESMQVLARNMSVLQS
                     SMDKKELLEALDRMDDAVDESMKRLPESISPADEEGKNDYIKELQKLEKEIMLAKQKV
                     LSGQLSDIPDSVNKMNKIRVEGHDKFR"
     gene            540..696
                     /locus_tag="N7B39_005193"
                     /pseudo
     CDS             540..696
                     /locus_tag="N7B39_005193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558160.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    596..>696
                     /locus_tag="N7B39_005193"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT          231 a          111 c          181 g          173 t
ORIGIN      
        1 cgtggaataa agttatgaat agcgatcggt ttgatttaaa caataaatga taaaactgat
       61 gagggagggc acctccctca tgaaggagaa attatgcaga aaagcaataa agttataatc
      121 agctgtggta tggcattata ttttttgtgt ggggggatta cagtggcaga agaacttgat
      181 gaaagcatgc aagttcttgc aaggaatatg agtgtacttc agtcatcaat ggataaaaag
      241 gagcttcttg aagctcttga cagaatggat gatgctgtag atgagtcaat gaagcgtctt
      301 cctgagagca tttcaccggc tgatgaagaa gggaagaatg actatataaa agaacttcaa
      361 aagcttgaaa aagagattat gctggcgaaa caaaaagtct tatcgggaca gttatcagat
      421 atacccgatt cggtaaacaa gatgaataaa atcagagttg aggggcatga taaattcaga
      481 taaatcagtt ggttgattcc tgcgggacga atacatacgt caggaacata actttatggc
      541 tggaacgtga aaatcgtgaa ctgcgccgca gtaacgatat ccttcgccag gcttccgctt
      601 attttgcgaa ggcggagttc gaccgcctct ggaaaaaata atgccactgc tggataagct
      661 gcgtgagcag tacggggtcg gaccggtatg cagtga
//
LOCUS       DBFIFI010000530          694 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.536, whole genome shotgun sequence.
ACCESSION   DBFIFI010000530 DBFIFI010000000
VERSION     DBFIFI010000530.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.536"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            61..>694
                     /gene="espR4"
                     /locus_tag="N7B39_005194"
     CDS             61..>694
                     /gene="espR4"
                     /locus_tag="N7B39_005194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector leucine-rich repeat protein EspR4"
                     /protein_id="HGU2424937.1"
                     /translation="MFPLNDLSLKTQSVQLNKITSNTESTIKQHELVSDDAIINELSS
                     ELVSCLGNDKFTPVSEDCNLLNMLSEFKLLREQCFRWGNYTLLFENYEAYDKTGSITI
                     EKNQGEGTLPIRHKLEFISTNIAELLDKLTKITDARLCKGFSDWASSVKEGGSNDLKE
                     NVDRALVRMFKCVKLHSNELDLSYLFLGSVPPLPDWIEMLSLVYNELDSIQ"
BASE COUNT          227 a           99 c          150 g          218 t
ORIGIN      
        1 taattttatt gtgttttaga atgcgccctg ggcaatataa tttattaaac aggagtgtaa
       61 atgtttccat taaatgatct gtcgttaaaa actcagtcgg ttcaattaaa taaaatcaca
      121 tcgaatactg agtctacgat aaaacaacac gagttagtat ctgatgatgc aatcataaat
      181 gaattatcaa gtgagttagt cagttgtctt ggaaatgata agtttacacc agttagtgaa
      241 gactgcaact tactgaatat gctgtctgaa tttaagttat tgagagagca atgtttcagg
      301 tggggtaatt atactctatt gtttgaaaat tatgaggctt atgataagac ggggtctatc
      361 acgatagaaa aaaatcaggg ggaggggact ttacccattc ggcataaatt agagtttata
      421 tcgactaata ttgcagagtt gctggacaag ttaacgaaaa ttacagatgc cagactttgc
      481 aaaggcttca gtgactgggc tagttcagtc aaagaaggcg gatcgaatga cctcaaagaa
      541 aatgtagata gagcgttggt gagaatgttt aaatgtgtta agcttcacag taatgaactt
      601 gacttatcat acctattttt gggttctgtg ccgcctcttc cagattggat tgaaatgctt
      661 agccttgttt ataatgaact tgattcaata cagg
//
LOCUS       DBFIFI010000531          691 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.537, whole genome shotgun sequence.
ACCESSION   DBFIFI010000531 DBFIFI010000000
VERSION     DBFIFI010000531.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.537"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..593)
                     /gene="upp"
                     /locus_tag="N7B39_005195"
     CDS             complement(<1..593)
                     /gene="upp"
                     /locus_tag="N7B39_005195"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HGU2424938.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLG"
BASE COUNT          146 a          194 c          163 g          188 t
ORIGIN      
        1 ccgaggcccg gaataatgta tccgtgctcg ttcagtccct gatcaatcga tgcggtatac
       61 agttcgacgt ccgggtgcgc tttttccagc gcagcgatac cttctggcgc agctaccagc
      121 accagaactt tgatgctgct gcagcccgct tttttcagca ggtcgatggt cgcaataacg
      181 gaaccaccgg ttgccagcat tgggtcaacg atcagcgcca tacgctcatc gatgttagaa
      241 accagtttct ggaagtacgg taccggctcc agcgtttctt cattacggta cataccgaca
      301 acgctgatgc gcgcgctcgg aacgttttcc agcacaccgt ccatcatccc cagacccgca
      361 cgcagaattg gcacaacggt aattttctta cctttgatct ggtcgatttc taccgggccg
      421 ttccagcctt cgatagttac tttttccgtt tcgaggtcgg cggtcgcttc gtaagtcagc
      481 aggctaccca cttcggaagc gagttcgcga aagcgcttgg tgctgatatc ttgctcacgc
      541 atcagtccca gcttgtgttt gacgagtggg tgtttgactt ccacgatctt catactcttt
      601 ctcctttgag gggcagccac aaaaaaaatc gacggattat acctcctttc ttcaaggcgg
      661 caatattatt ttcgttgact ttagtcaaaa t
//
LOCUS       DBFIFI010000532          686 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.538, whole genome shotgun sequence.
ACCESSION   DBFIFI010000532 DBFIFI010000000
VERSION     DBFIFI010000532.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.538"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..79)
                     /locus_tag="N7B39_005196"
     CDS             complement(<1..79)
                     /locus_tag="N7B39_005196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HGU2424939.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTS"
     gene            complement(91..489)
                     /locus_tag="N7B39_005197"
     CDS             complement(91..489)
                     /locus_tag="N7B39_005197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HGU2424940.1"
                     /translation="MTKDELIARLRSLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DETAGQDTPLSRENVLTGHENEVGSAQPDTVILDTSELVTVVALVKLHTDALHVTRDE
                     PVAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            complement(531..>686)
                     /locus_tag="N7B39_005198"
     CDS             complement(531..>686)
                     /locus_tag="N7B39_005198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HGU2424941.1"
                     /translation="ADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
BASE COUNT          140 a          218 c          178 g          150 t
ORIGIN      
        1 ctgacgttcc catgtacccg cgtatcgttt catcggcgcg ggcaatggca gcatcgaaca
       61 ggttatcgaa atcagccaca gcgcctcccg ttattgcatt ctggccaggc cgcgctctgt
      121 catttcggct gccacaccgg cagagacacg aaacgccgtt cccggcagca caaatgccac
      181 aggttcatcc cgcgtgacgt gaagtgcatc agtatgcagc ttcaccagtg ccacgaccgt
      241 gaccagttca gacgtatcca gaatcacggt atccggctgc gctgatccca cctcattttc
      301 atgtccggtc agcacatttt cccggctgag aggggtgtcc tgaccggcag tttcatccgt
      361 gtcatcaagc tcctcttcca gctctgccac acggagcgcc agttcttctt tcgtccccgt
      421 caggctgaca tcacggttca gttgttcacc cagcgagcgg agacgggcaa tcagttcatc
      481 tttcgtcatg gactcctcca cagaaaaaca atggccccga agggccatga ttacgccagt
      541 tgtacggaca cgaactcatc agggtcagcc agcagcatca gcggtgctga ctgaatcatg
      601 gtgaactcac gcgccggatc gccggtggtc acccagtttt tcgggtaacg ggcagaggcg
      661 ttaatgcctt cgcgctgtgc gtccgc
//
LOCUS       DBFIFI010000533          685 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.539, whole genome shotgun sequence.
ACCESSION   DBFIFI010000533 DBFIFI010000000
VERSION     DBFIFI010000533.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.539"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            82..684
                     /gene="fimB"
                     /locus_tag="N7B39_005199"
     CDS             82..684
                     /gene="fimB"
                     /locus_tag="N7B39_005199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HGU2424942.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT          196 a          132 c          157 g          200 t
ORIGIN      
        1 taaacataat caggataaaa atgttggatt attgctaacc tagcacagct agtgcgcgtc
       61 tgtaattata agggaaaaac gatgaagaat aaggctgata acaaaaaaag gaacttcctg
      121 acccatagtg aaatcgaatc actccttaaa gcagcaaata ccgggcctca tgcagcacgt
      181 aattattgtc tgactttgct ttgttttatt catggtttcc gggcgagtga aatttgtcga
      241 ttgaggattt cggatattga tcttaaggca aagtgtatat atatccatcg attaaaaaaa
      301 ggcttttcaa caacgcaccc gctattgaac aaagaagttc aggctttaaa aaactggttg
      361 agtatccgta cttcttatcc gcatgctgag agcgagtggg tatttttatc gcgtaagggg
      421 aatccgcttt ctcggcaaca attttaccat attatctcga cttccggtgg taatgccggg
      481 ttgtcactgg agattcatcc gcacatgtta cgccattcgt gtggttttgc tttggcgaat
      541 atgggaatag atacgcgact tatccaggat tatcttgggc atcgcaatat tcgtcatact
      601 gtctggtata ctgccagcaa tgcagggcgt ttttacggca tctgggatag agccagagga
      661 cgacagcgtc acgctgtttt ataga
//
LOCUS       DBFIFI010000534          684 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.540, whole genome shotgun sequence.
ACCESSION   DBFIFI010000534 DBFIFI010000000
VERSION     DBFIFI010000534.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.540"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..428
                     /locus_tag="N7B39_005200"
     CDS             <1..428
                     /locus_tag="N7B39_005200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HGU2424943.1"
                     /translation="KSTVSVFYLQPEEVNTLSHQAKRGDGEAGFRLFLYYKLSNYDEE
                     KSSQWLKIAANNGHATAQYRLYVELQEQGETKEANEWLQKIKLAASNNDSSAIWFLVN
                     CYKNQGNKKEALQWAYKLKASKQPLDADRLIEELTKEQK"
     gene            complement(431..>684)
                     /locus_tag="N7B39_005201"
     CDS             complement(431..>684)
                     /locus_tag="N7B39_005201"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424944.1"
                     /translation="KIYAESCAGHILNDTKSYIFIFSLATFDASAKYIVMITGDEDAS
                     FGFNLRFDELVAVVVNVILCVGVVATGKVFLLSIFLLPV"
BASE COUNT          260 a          115 c          145 g          164 t
ORIGIN      
        1 caaaatcaac agtaagcgtc ttttatttgc agccagaaga agtaaatact ttatcacacc
       61 aggcaaaacg aggcgatggc gaggcgggtt ttaggttgtt cttgtactac aagttgtcca
      121 attatgatga agaaaaaagt agtcagtggt taaaaatagc ggctaataat ggtcatgcga
      181 cagctcagta tcggttatat gtagagttac aagaacaagg tgaaaccaag gaagctaatg
      241 aatggttaca aaaaattaaa ttggctgcca gtaataatga cagttcagca atatggttct
      301 tggtgaattg ttataaaaac cagggaaata aaaaagaagc attgcaatgg gcatataaac
      361 tcaaagcgag taaacagccg cttgatgcag atcgactaat tgaagaatta acgaaagaac
      421 aaaagtagct ttaaacaggt aacagaaaaa tggagagtag aaaaacttta ccagtcgcta
      481 caacacctac acacaaaatt acgtttacga cgactgcaac aagctcatca aagcgcaagt
      541 tgaaaccaaa actggcatct tcgtcgccgg ttatcattac gatgtacttg gcagatgcat
      601 caaaagtcgc aagagaaaag atgaaaatat agctttttgt atcattaagt atgtggccag
      661 cacatgactc ggcatagatc ttat
//
LOCUS       DBFIFI010000535          680 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.541, whole genome shotgun sequence.
ACCESSION   DBFIFI010000535 DBFIFI010000000
VERSION     DBFIFI010000535.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.541"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(1..336)
                     /locus_tag="N7B39_005202"
                     /pseudo
     CDS             complement(1..336)
                     /locus_tag="N7B39_005202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347735.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          175 a          169 c          135 g          201 t
ORIGIN      
        1 tgattctgga aaaggacttt gtggattgtt gctattgcca gtgagattga atgaagtcgt
       61 tttaactcgc tttgaatacg tcttgccccc agatttcgct gagaacgtaa ctccagaatc
      121 agagcgactt cgcaagtgcc ggttttcgtt gatggtgaac gcttggggcg acggctgtgg
      181 ctttccagac cagcgatgcc ctgagccaga tatctgcgcc accattttac gcaatgtggg
      241 ccgagaaatg ccacagcgac ggcatacaaa tcccgcatca ccggacgttt catagagttt
      301 tacccactgt agccgctgct gaatttccct gttcataatt tggcattagt tcagctcaat
      361 cccgacttaa aaagtcccta caggatgttc cggacctgag gcacagttca tatccttatc
      421 cataaaaaat ttagctatgt ctatagcatt aatcttgaca agacgacgtt agggcctggc
      481 gatgaatcat ttcccctgcc agatatcctt tggggtgcac cagaactacc gttttatatc
      541 tatataactg aaacgtccct cgccatcctt catattttga tgtagtaaca ccattcactt
      601 tcatctaccc cccgttttat taacatatag taaaaatcac ctcgccgcat tttattcttt
      661 tgaagtaaaa tcatttacca
//
LOCUS       DBFIFI010000536          680 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.542, whole genome shotgun sequence.
ACCESSION   DBFIFI010000536 DBFIFI010000000
VERSION     DBFIFI010000536.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.542"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            163..525
                     /locus_tag="N7B39_005203"
     CDS             163..525
                     /locus_tag="N7B39_005203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HGU2424945.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT          199 a          152 c          156 g          173 t
ORIGIN      
        1 ttatcttttt tctatagatt tcttaattaa tcaataaatt gcgtagtttt taagcatgtt
       61 aatgattacg ttacagaact attgcaatgt catcagttgt gaacaaagca cctggtcgcg
      121 catactaggg gctataaatt tatctttatc agaagccatc acatgaaaga agtcgaaaaa
      181 aacgaaatca aacgtctcag cgatcgtctg gacgccatcc gccaccagca ggccgatctg
      241 tcgctggttg aagccgcaga caaatatgcc gagctggaaa aagagaaagc cacgctggaa
      301 gcagaaattg ctcgcctgcg tgaagttcat agccaaaaac tgagtaaaga agcacagaag
      361 ctgatgaaga tgccgtttca gcgcgcaatt accaaaaaag agcaggctga tatgggcaag
      421 ctgaagaaaa gtgttcgcgg actggtggtt gtgcacccaa tgaccgcgct gggccgtgaa
      481 atgggcctgc aggagatgac tggattctct aagactgcgt tttaagcgcc tcagacgact
      541 ggttcgatca tatcaacccg catctggctt gccttagctg gtcaatgcgg ggactctcct
      601 gtctactcgc ctaattacca aacaattcac tccctttacg tcgatttacc gctggttgat
      661 attctgaaaa tgcattttta
//
LOCUS       DBFIFI010000537          676 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.543, whole genome shotgun sequence.
ACCESSION   DBFIFI010000537 DBFIFI010000000
VERSION     DBFIFI010000537.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..676
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.543"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..>676)
                     /locus_tag="N7B39_005204"
     CDS             complement(<1..>676)
                     /locus_tag="N7B39_005204"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGU2424946.1"
                     /translation="MHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLANADIEIVPNGID
                     LETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKLAAAHKNLKLVV
                     IGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHCYYPLADLVVIPSQFQ
                     EPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSISSDILKTLANP
                     ELTAVA"
BASE COUNT          197 a          140 c          113 g          226 t
ORIGIN      
        1 tttgcaactg ctgttaattc aggattcgct aacgttttta aaatatcgct gcttatggag
       61 tctgcagtca taggttcttt taaatggaat cccgtagtat tttctttgac aaattccgtc
      121 atccccccac gcgtactcac taaaacaggc ttaccagcac ccatagcttc aattgccacc
      181 atgcaaaatg gttcctggaa ctgggaagga atgacgacta aatctgccag tgggtaataa
      241 caatgcatct tttcaggcgg tatgcttcct aacataatgc aacgatcctt caaacgtttt
      301 gcaatttccc tgacattgcg ttgatatgca cctttgtcac ttttactcat ttctgtataa
      361 tcaccaatca caactaattt taaatttttg tgagcagcgg ccaatttttc gaatgattgc
      421 atcaacagca atatgccttt gtcagggacg atacgcccag cataaaaaat aattttctct
      481 tcaggggaga tattaagttc gcttctagtg atcggctgaa aattagattg ataggtttct
      541 aaatcaattc catttggtac aatctcaata tctgcatttg ctaaataaga ttggtaatac
      601 tttttcaagt ataaactagg cactatcatt ttaacatttt gctcaagcaa ttttggttca
      661 aatgcattat gcatat
//
LOCUS       DBFIFI010000538          674 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.544, whole genome shotgun sequence.
ACCESSION   DBFIFI010000538 DBFIFI010000000
VERSION     DBFIFI010000538.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..674
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.544"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(160..543)
                     /gene="traM"
                     /locus_tag="N7B39_005205"
     CDS             complement(160..543)
                     /gene="traM"
                     /locus_tag="N7B39_005205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008455364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome DNA-binding protein
                     TraM"
                     /protein_id="HGU2424947.1"
                     /translation="MPRIQTFVSNQVREEIESLVTERRQEGATENDATVSSVTSMLIE
                     LGLRVYRIQREKKESGFNQMEYNKIMLDNIARVRAMCSEIMKMNALSHEVISNGNFDN
                     EVMKRSITKFAEDQVRIFFPEEEMD"
BASE COUNT          207 a          154 c          100 g          213 t
ORIGIN      
        1 tatgatacca ctgcccctta aaaccaactt cattaagaaa acagaaaagc aactcgccat
       61 tttatcaaca atcagttcgg gttaaataaa aatgttctcc ctagagggcc tcccacaaac
      121 agataaaaaa acaggaggca tgagtgacat ttcgtagact taatccattt cttcttctgg
      181 aaagaaaatc cttacctggt cttctgcgaa tttggttata gatcttttca ttacttcatt
      241 atcaaaatta ccattgctta taacttcatg acttaatgca ttcatcttca ttatttctga
      301 acacattgcc ctgactctgg ctatgttatc cagcataatc ttgttgtact ccatctgatt
      361 aaaaccactc tccttctttt ctctctgaat cctgtatact ctgagaccga gttctataag
      421 cattgaagta acagacgata ccgtagcatc gttttccgtc gccccctctt gcctcctttc
      481 tgttaccaga gattcaattt cttcacggac ctgattactg acaaaagttt gtatgcgtgg
      541 catcgctaat ccttttcaaa ccaacaacca tcttcaatat aaaatattgt ctgaataaat
      601 ttatccatag tataaggcga gtataccatt aaaataagca gagtatagat gcgagtatgg
      661 aaggctgcga agca
//
LOCUS       DBFIFI010000539          667 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.545, whole genome shotgun sequence.
ACCESSION   DBFIFI010000539 DBFIFI010000000
VERSION     DBFIFI010000539.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..667
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.545"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(247..443)
                     /locus_tag="N7B39_005206"
                     /pseudo
     CDS             complement(247..443)
                     /locus_tag="N7B39_005206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
BASE COUNT          201 a          170 c          131 g          165 t
ORIGIN      
        1 tgcatttggt ggccagaacg actgagccgg gcttaagtga caacgtatcc ttttcaagaa
       61 aaatattaat tggggagcaa accatgataa ctgaacagac aacaaccatt ataattttac
      121 tttcattagc aattggttag ttcaattata gccccaaaag gtaaattatc atcaacacat
      181 aagcaaagga ctgacaggtg ccgctaacac ccaccagccg cccatttacc acaaataaaa
      241 aagccttcag gactgaaggc gtctgtaaca accgaactga tagtctgcca gacccgcata
      301 accagctggg tcagtattaa ctggcagcgc tcgcgtgaaa ggtacgtatt ctgcgcaatc
      361 tccccgaccg tcgccggttc ggtgacgctt aattcatcaa acacaactct ggcggtttct
      421 gtcatatcct gctgtttcag catgtctttt taccctttcc ggttaacgtg acacaccaat
      481 aactcttgtc gaaaaagcca gcaagctgaa agacctgtat taataactac cagcacattt
      541 aacgcactgc gctactttgc gggcacaaaa aacccgctca gaggcggggt caagctatgc
      601 ggcgaaataa ccactcttaa cagcatacct gattttttac gtacgtaaat gctttgccgt
      661 gcatatt
//
LOCUS       DBFIFI010000540          650 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.546, whole genome shotgun sequence.
ACCESSION   DBFIFI010000540 DBFIFI010000000
VERSION     DBFIFI010000540.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.546"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..508
                     /gene="nleB"
                     /locus_tag="N7B39_005207"
     CDS             <1..508
                     /gene="nleB"
                     /locus_tag="N7B39_005207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector arginine
                     glycosyltransferase NleB"
                     /protein_id="HGU2424948.1"
                     /translation="LKNIPEGKDSLIESYAEKREHTWFDFFRNLAMLKAGSLFTETGK
                     TGCHNISPCSGCIYLDADMIITDKLGVLYAPDGIAVHVDCNDEIKSLENGAIVVNRSN
                     HPALLAGLDIMKSKVDAHPYYDGLGKGIKRHFNYSSLHDYNAFCDFIEFKHENIIPNT
                     SMYTCSSW"
     gene            557..>650
                     /locus_tag="N7B39_005208"
     CDS             557..>650
                     /locus_tag="N7B39_005208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein NleE"
                     /protein_id="HGU2424949.1"
                     /translation="MINPVTNTQGVSPINTKYAEHVVKNIYPEIK"
BASE COUNT          205 a           98 c          138 g          209 t
ORIGIN      
        1 gttaaaaaat atcccagaag gtaaggactc tttgattgag tcatatgcag aaaaaagaga
       61 acatacttgg tttgattttt tcaggaattt ggccatgttg aaggctggaa gtttgtttac
      121 agagacggga aaaactggat gccataacat atcgccctgt agcggatgta tatatcttga
      181 tgccgacatg attattaccg ataaattagg agtcctgtat gctcctgatg gtatcgctgt
      241 gcatgtagat tgtaatgatg agataaaaag tcttgaaaat ggtgcgatag ttgtcaatcg
      301 tagtaatcat ccagcattac ttgcaggcct cgatattatg aagagtaaag ttgacgctca
      361 tccatattat gatggtctag gaaagggtat caagcggcat tttaactatt catcgttaca
      421 cgattataat gctttttgtg attttattga atttaagcat gaaaatatta taccgaatac
      481 cagtatgtat acctgcagtt catggtaagt cttccggttg tggttgaaaa atatcgttct
      541 ttaggtatag attcagatga ttaatcctgt tactaatact cagggcgtgt cccctataaa
      601 tactaaatat gctgaacatg tggtgaaaaa tatttacccg gaaattaaac
//
LOCUS       DBFIFI010000541          628 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.547, whole genome shotgun sequence.
ACCESSION   DBFIFI010000541 DBFIFI010000000
VERSION     DBFIFI010000541.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.547"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..74)
                     /locus_tag="N7B39_005209"
     CDS             complement(<1..74)
                     /locus_tag="N7B39_005209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424950.1"
                     /translation="MKRVLFFLLMIFVSFGVIADCEIQA"
     gene            complement(71..>628)
                     /locus_tag="N7B39_005210"
     CDS             complement(71..>628)
                     /locus_tag="N7B39_005210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HGU2424951.1"
                     /translation="GRYITQDPIGLKGGWNFYQYPLNPVQYIDSMGLASKYGHLNNGG
                     YGARPNKPPTPDPSKFPDIAKQLRLPYPIDQASSAPNVFKTFFRALSPYDYTLYCRKW
                     VKPNLTCTPQDDPQYPGMDTKTASDYLPQTNWPTTQLPPGYTCAEPYLFPDINKPDGP
                     ATAGIDDLGEILAKMKQRTSRGIRK"
BASE COUNT          159 a          138 c          123 g          208 t
ORIGIN      
        1 gcttgtattt cgcaatcagc tataacacca aaactaacaa atatcatcag caaaaaaaac
       61 aaaactcttt tcattttctt attcctctcg atgttctctg tttcatttta gctaaaattt
      121 cacccaaatc atctatccct gctgttgctg gcccatcggg tttattaatg tcagggaata
      181 aatagggttc tgcacaagta tatcctggtg gtaattgagt tgttggccaa tttgtctgtg
      241 gcaggtaatc acttgctgtc tttgtatcca tccctggata ctggggatca tcctgtggcg
      301 tacaagtcag atttggtttt acccacttcc tgcaatacag tgtgtagtcg taagggctta
      361 atgctctgaa gaatgttttg aaaacattag gcgcactact ggcctggtca ataggatatg
      421 gcagtcttaa ttgtttcgct atgtctggaa atttacttgg atcgggcgta ggcggtttgt
      481 tgggtctcgc tccatatccg ccattattta agtgtccata ttttgatgcc agtcccattg
      541 aatctatata ctgaaccgga ttcaacggat actgataaaa attccatccc cccttcagtc
      601 caatcggatc ctgagtgata tacctccc
//
LOCUS       DBFIFI010000542          612 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.548, whole genome shotgun sequence.
ACCESSION   DBFIFI010000542 DBFIFI010000000
VERSION     DBFIFI010000542.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.548"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..>612
                     /locus_tag="N7B39_005211"
     CDS             <1..>612
                     /locus_tag="N7B39_005211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGU2424952.1"
                     /translation="DANFIYYYLRSPVFVCYVENCQTGVAYPAINDKQFFSGITPVPP
                     SLEQVRIANKIKELMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQTAEELAENWAR
                     ISEYFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKRIAQEKAQLVKE
                     GKIKKQKPLPPISDEEKPFELPEGWEWCLFEDIIDIQSGITKG"
BASE COUNT          195 a          132 c          134 g          151 t
ORIGIN      
        1 tggatgctaa tttcatttat tattatctac gttctccggt atttgtttgt tatgttgaaa
       61 attgtcagac aggggttgcc tatccagcaa tcaatgacaa acaatttttt tctggaataa
      121 ctccagttcc tccatccttg gaacaagttc gcatcgcaaa caaaatcaaa gaattaatgt
      181 ccctctgcga ccaactggaa cagcaatccc tgaccagtct ggacgcacat caacaactgg
      241 ttgaaaccct gttgggaacg cttacagaca gccaaaccgc cgaggaactg gctgaaaact
      301 gggcgcgtat tagcgagtat ttcgacactc tatttaccac cgaagccagc gtggatgcgt
      361 taaaacagac cattctgcaa ctggccgtaa tgggcaaact tgtgccgcag gatccgaatg
      421 atgaaccagc ctctgaactg ctcaaacgaa ttgcgcagga aaaagctcaa ctggtgaaag
      481 aaggaaaaat aaaaaaacaa aaaccgttgc cgccaattag cgatgaggaa aaaccgtttg
      541 aacttccgga agggtgggag tggtgtttat ttgaagatat tattgatatt caaagtggta
      601 tcactaaagg aa
//
LOCUS       DBFIFI010000543          603 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.549, whole genome shotgun sequence.
ACCESSION   DBFIFI010000543 DBFIFI010000000
VERSION     DBFIFI010000543.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.549"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..>603)
                     /locus_tag="N7B39_005212"
     CDS             complement(<1..>603)
                     /locus_tag="N7B39_005212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HGU2424953.1"
                     /translation="ESLNESVKSEQSPRVVLWEIDLTAQGGERYFFCNELNEKGEAVT
                     WQGRQYQAYPIDGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGATVVRRRV
                     YARFLDAVNFVAGNPEADPEQELTDRWVVEQMSSLTAMTASFVLATPTETDGALFPGR
                     IMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCS"
BASE COUNT          117 a          222 c          139 g          125 t
ORIGIN      
        1 ttactgcatc tgtccttccg gatatccgtg gtggggttgt cgaactcatc cgccactgcc
       61 ggaccgttat acccgcattc atcgccccgg taatcccaca tacaggtgtt cgccagcatg
      121 atgcgaccgg gaaacagcgc cccgtccgtc tccgtcggtg tcgccagcac aaacgaggcc
      181 gtcatggccg tcagcgatga catctgctcc accacccacc ggtccgtcag ctcctgctca
      241 gggtctgcct caggattgcc tgccacaaaa ttcaccgcat ccagaaaacg ggcatacacc
      301 cggcggcgga ccaccgtggc ccccaccagg ctctgcaggt cctccgccat cccggtgacc
      361 agaccaaaca gattcgacac cgtcagcgac ggtctggcac tgctgccctt cccgttcatc
      421 tcaaagccac tgccgtcaat cgggtatgcc tgatattgcc gcccctgcca ggtaaccgcc
      481 tccccttttt cattcagctc attgcagaaa aaataccgct caccgccctg cgccgtcagg
      541 tcgatttccc agagtaccac ccgcggtgac tgctctgatt taaccgactc gttcagactt
      601 tct
//
LOCUS       DBFIFI010000544          602 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.550, whole genome shotgun sequence.
ACCESSION   DBFIFI010000544 DBFIFI010000000
VERSION     DBFIFI010000544.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.550"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(17..>602)
                     /locus_tag="N7B39_005213"
     CDS             complement(17..>602)
                     /locus_tag="N7B39_005213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HGU2424954.1"
                     /translation="HIARSNQNDTITIGGRNMRLHYIQFMDGFSVEPILGGLWDHLGA
                     REAHHLADRLTRQLNGGNTFLQAYSLYLEQRQAAPLVQESVIKTLLDRINSNAFPVSL
                     QDFSCTEEHLNCPITLHIPETGVFVRNARNSEICALYDQEALTELILRNALHPLSRDP
                     FAPEMIISKDKCHFNITKQCFYALPIYPLQQNSI"
BASE COUNT          165 a          115 c          130 g          192 t
ORIGIN      
        1 tattgtgctt ttatttttaa atactgtttt gttgaagtgg gtatatgggt aatgcgtaaa
       61 aacattgttt tgttatatta aaatgacact tgtctttgct tataatcatt tctggagcaa
      121 aggggtcacg gctgaggggg tggagagcgt tacgcaggat aagttcagtc aatgcttcct
      181 gatcatataa tgcacatatt tctgaatttc gagcatttct gacaaaaaca cctgtctcag
      241 gaatatgtag cgttatcgga caattaagat gttcctcagt acaggaaaag tcttgtaaac
      301 taacgggaaa tgcattcgaa tttattcgat ctagtagtgt ttttatgacg ctctcctgca
      361 caagcggggc agcttgcctc tgctccagat ataagctgta tgcctgcaga aaagtgttac
      421 ctccgttaag ttgtcttgtc aggcggtctg cgagatgatg agcctctctt gccccaagat
      481 ggtcccaaag tccaccaaga ataggctcaa cactaaaacc atccataaac tggatataat
      541 gaagcctcat attacggcct cctatagtaa tagtgtcatt ctggttgctt cttgctatat
      601 gt
//
LOCUS       DBFIFI010000545          600 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.551, whole genome shotgun sequence.
ACCESSION   DBFIFI010000545 DBFIFI010000000
VERSION     DBFIFI010000545.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..600
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.551"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..110
                     /locus_tag="N7B39_005214"
     CDS             <1..110
                     /locus_tag="N7B39_005214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424955.1"
                     /translation="HNAAWGVRTLWFELVTKIDIDIHKKNRYASYDLDR"
     gene            complement(210..425)
                     /locus_tag="N7B39_005215"
                     /pseudo
     CDS             complement(210..425)
                     /locus_tag="N7B39_005215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(469..>600)
                     /locus_tag="N7B39_005216"
     CDS             complement(469..>600)
                     /locus_tag="N7B39_005216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGU2424956.1"
                     /translation="NRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMAA"
BASE COUNT          163 a          145 c          136 g          156 t
ORIGIN      
        1 ttcataacgc agcatgggga gtgcgaacat tgtggtttga actcgtcaca aaaatagata
       61 tagatataca taaaaagaac agatatgcga gttatgatct tgatcgatag gaatttaaat
      121 cccaaaaaga ttaaaaaaac acccaaaaac ggatgtttat tcaacactgc ctgcgtgcca
      181 tgagtgcacc gccgtcaatt aaatggatct atttcatcct gaccagcata gtgctccaga
      241 cggctcatcg gcaccggcgg ttttttcagg taagagccga agtacaccgc cacatgtgtg
      301 gcgttatcca tcacccggga tacgttgaca ttccagcccc gccggtaatg actgtccagg
      361 aagcgattcc agtcccgttt actgcttcct tctgctgcca gcgcatccgg tatcaccagg
      421 tccggtgagg tgtactggca atagcggaca ctaccatttg ttcttttttt aagcagccat
      481 ctgatgatat ttttccctga aggctgccgg ggagatattc cccagacgag agtgacgacg
      541 ctgacgattg tagaaaatct caatgtattc ccgtattact gagatggctt catcccggtt
//
LOCUS       DBFIFI010000546          598 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.552, whole genome shotgun sequence.
ACCESSION   DBFIFI010000546 DBFIFI010000000
VERSION     DBFIFI010000546.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.552"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            58..>598
                     /locus_tag="N7B39_005217"
     CDS             58..>598
                     /locus_tag="N7B39_005217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HGU2424957.1"
                     /translation="MNIYERIDGSKYRNIWVVGDLHGCYTNLMKKLETIGFDTKKDLL
                     ISVGDLVDRGTENVECLELITFPWFRAVRGNHEQMMIDGLSERGNVNHWLLNGGGWFF
                     NLDYDKEILAKALAHKADELPLIIELVSKGKKYVICHADYPCDKYEFGKPVDHQQVIW
                     NRERISNSQDGIVKEIKGAD"
BASE COUNT          199 a          116 c          149 g          134 t
ORIGIN      
        1 caaggcggag tatcaacaga aacttaaaaa actgcgaaac agcagaagtg aggttgcatg
       61 aatatctacg aaagaattga tggcagcaaa taccgaaata tttgggtagt tggcgatctg
      121 cacggatgct acacgaacct gatgaaaaaa ctggagacga taggattcga caccaaaaaa
      181 gacctgctta tctcggtggg cgatttggtt gatcgcggta cagagaacgt agaatgcctg
      241 gaattaatca cattcccctg gttcagagct gtacgtggaa accatgagca aatgatgatt
      301 gatggcttat cagagcgtgg aaacgtcaat cactggctgc ttaatggcgg tggctggttc
      361 tttaatctcg attacgacaa agaaattctg gctaaagctc ttgcccataa agcagatgaa
      421 cttccgttaa tcatcgaact ggtgagtaaa ggaaaaaaat atgtcatctg ccacgccgat
      481 tatccttgtg ataaatacga gtttggaaag ccagttgatc atcagcaggt aatctggaac
      541 cgcgaacgaa tcagcaactc acaagacggg atcgtgaaag aaatcaaagg cgcggaca
//
LOCUS       DBFIFI010000547          592 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.553, whole genome shotgun sequence.
ACCESSION   DBFIFI010000547 DBFIFI010000000
VERSION     DBFIFI010000547.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.553"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..273
                     /locus_tag="N7B39_005218"
     CDS             <1..273
                     /locus_tag="N7B39_005218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HGU2424958.1"
                     /translation="EPSAEAWYLWQEVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDV
                     LCDTDLQRVFTPDDREQVLAVYGPVHARLLRQALELIADAESARKK"
     gene            369..578
                     /locus_tag="N7B39_005219"
     CDS             369..578
                     /locus_tag="N7B39_005219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HGU2424959.1"
                     /translation="MWMEFDRVSPLGDERGDIRNAQIVKAVFGAQGMNVALKDAMLCW
                     GEDEDKPEVDPFAALEDALSLAAMS"
BASE COUNT          125 a          139 c          205 g          123 t
ORIGIN      
        1 gagccttcgg cagaggcctg gtatctgtgg caggaagtgc tcaatggtga tggagaggat
       61 gacgataccc tgtcggtggt ggcgaaaacc cgccgtaacc tggaagcgga tgtgacgctg
      121 ttctgcgatg tcctgtgtga tactgacctg caacgggtgt tcactccgga cgaccgtgag
      181 caggtgctgg ccgtctatgg tccggtacat gcccggttgc tgcgtcaggc actggaactg
      241 atcgctgatg cagagtcggc cagaaaaaag tagcccgccc ggaaattcgc tttctgatgc
      301 gacttgcgct ccgtctgggg cgcaccttat ccgaactgcg gcacagcctg agtgcgagcg
      361 aggcgatgat gtggatggag ttcgacaggg tatccccgct gggtgatgag cgcggggata
      421 tccgtaatgc acagatcgtg aaagcggttt tcggggcaca ggggatgaat gttgcactga
      481 aggacgccat gctctgctgg ggcgaggatg aggataagcc ggaggtggat ccgtttgcgg
      541 cgctggaaga cgcgctgagc cttgcagcaa tgtcataaat aatgatgaaa ac
//
LOCUS       DBFIFI010000548          591 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.554, whole genome shotgun sequence.
ACCESSION   DBFIFI010000548 DBFIFI010000000
VERSION     DBFIFI010000548.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.554"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..75
                     /locus_tag="N7B39_005220"
     CDS             <1..75
                     /locus_tag="N7B39_005220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HGU2424960.1"
                     /translation="QIVVDEIRDTLEKAVIDCCDEADM"
     gene            72..>591
                     /locus_tag="N7B39_005221"
     CDS             72..>591
                     /locus_tag="N7B39_005221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HGU2424961.1"
                     /translation="MNQVNESHSRASDIWREVASLFRPPSRLPVAEAIRRYMRVPRGA
                     NTSGPWESSLTPYMIDPINTLSAREYDAVVFVGPARTGKTEGLIDGWIVYGIICDPAD
                     MLVVQMTETKAREHSRTRLSRTFRHSPEVSKRLSPSRNDNNVHDKMFLDGSFLKIGWP
                     SITVFSSSDYRRV"
BASE COUNT          127 a          151 c          173 g          140 t
ORIGIN      
        1 cagattgtgg ttgatgagat ccgcgacaca ctggaaaagg cagtcattga ctgttgtgat
       61 gaggccgata tgtgaatcag gtgaacgaga gccatagccg cgcatccgat atctggcgcg
      121 aagtggcctc gctgtttcgc ccacccagcc ggttaccagt agcggaagcc atcaggcgtt
      181 atatgcgggt tccacgggga gccaatactt ccggtccgtg ggagtcatcg ctgacgccct
      241 atatgattga ccccattaat acattatcag cccgtgaata tgacgcggtg gtgtttgtgg
      301 gacctgcgcg aaccgggaaa accgaagggc tgattgatgg ctggattgtg tacggcatca
      361 tctgtgatcc ggcggatatg ctggtggtgc agatgactga gacgaaggcg cgtgagcatt
      421 ccagaacgcg tctttccagg acgtttcgcc acagtccgga ggtcagcaag cgcctcagtc
      481 cttcccgtaa tgacaacaac gtccacgata aaatgtttct tgacggctcc ttcctgaaga
      541 ttggctggcc gtcgatcacc gtcttttcct cttcggatta ccgtcgtgtg g
//
LOCUS       DBFIFI010000549          586 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.555, whole genome shotgun sequence.
ACCESSION   DBFIFI010000549 DBFIFI010000000
VERSION     DBFIFI010000549.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.555"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(58..237)
                     /locus_tag="N7B39_005222"
     CDS             complement(58..237)
                     /locus_tag="N7B39_005222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HGU2424962.1"
                     /translation="MATKAVNAKSKKLEARVPHAIADAVENLKEDGESTGQFIVSALE
                     GEIKRRQRRKAKEQE"
     gene            432..>586
                     /locus_tag="N7B39_005223"
     CDS             432..>586
                     /locus_tag="N7B39_005223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HGU2424963.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVA"
BASE COUNT          136 a          142 c          139 g          169 t
ORIGIN      
        1 tcatagcgat ttgttggctg gtgggcaatg tgatcagtgt gctggcttgc tggtgggcta
       61 ttcctgctct ttggccttgc gacgctggcg gcgtttgatc tcgccttcaa gtgccgaaac
      121 gataaattgc cctgtacttt cgccatcttc ctttaaattt tctacagcat cagctattgc
      181 gtgtggtact cgagcctcaa gctttttgga ttttgcatta actgcttttg ttgccatatc
      241 tgatttcctc gttattaggt ggctgacagt atacacacaa gaaaacggaa aaacactatt
      301 gacgtggctg acacctgtta ctaatatagt ggctgacacc tttatcaagg taatcgatga
      361 aaagacaaag ccccgcaagt gtcgttacca ctcgcagggc ttctaaccaa caacgtaaac
      421 taggagccgt tatggttgct gtaaatcata taccacacct tgtacacaca caaacggcct
      481 ttgtgtggcg ttttctggca ctgagtgccg gagaatctca aatcatccac gtaaccgcct
      541 ggacggaacg cgaagcgcgt agccgttgcc cgtcaggttg tgttgc
//
LOCUS       DBFIFI010000550          584 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.556, whole genome shotgun sequence.
ACCESSION   DBFIFI010000550 DBFIFI010000000
VERSION     DBFIFI010000550.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.556"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
BASE COUNT          182 a          126 c          127 g          149 t
ORIGIN      
        1 agctgaaccg tttcactata ctctacttaa ttcgagttac aggaaggcgg caagcgagtg
       61 aatccccagg agcttacata agtaagtgat tggggtaagc gaacgcagtc gcagcacatg
      121 caacttgaag tatgacgagt atatatatct attagctgtt tcccggacat aagctacagc
      181 cagcactaac ctggaacggg tgcattataa aaaggaacaa gggacaaata gctgaacagt
      241 aaccgcgtta gtctcttccc gcataaccgt accattcgta ttaagaagta ttgcccatat
      301 ccattcggcg aaagaggatc tgccatacat caagtccgtt ttacttggca tcagatcatc
      361 gccgtcatta actctgttaa agtcggacaa acgattagca agatctgtga ttcaaaagtg
      421 cttagcgcta aggtgggact ggttacagat tcattaaccc tgttctttgt cgcacctatc
      481 gatcggtgta atgccacaac aggtttatgt taatcagaac aatgcgcccc tctataaaga
      541 gaattctttg ataaggcaac ttgaaaaggg aaagctaact gata
//
LOCUS       DBFIFI010000551          584 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.557, whole genome shotgun sequence.
ACCESSION   DBFIFI010000551 DBFIFI010000000
VERSION     DBFIFI010000551.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.557"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..>584
                     /locus_tag="N7B39_005224"
     CDS             <1..>584
                     /locus_tag="N7B39_005224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HGU2424964.1"
                     /translation="VIVNSNGEQYYWVTKSDAQKLTKIGCYTRTAMDEMYNHLSVCLA
                     HNINEYFGIQETKYILDQLEMKYSDLLKEILRYITVQRISEVIQRLIQERISVRNMRL
                     VMEALALWSPREKDIITLVEHVRGALGRYICHKFSYSGEIKAIVISPEIEDRIRDGVR
                     PTAGGTFLNLDASEAEMILDNFKLALSGINIPIK"
BASE COUNT          189 a           94 c          123 g          178 t
ORIGIN      
        1 ctgtcattgt aaatagtaat ggtgaacaat attattgggt tacgaaaagt gatgctcaaa
       61 aattaacaaa aattggctgt tatactcgca cggccatgga tgaaatgtat aatcatttat
      121 ctgtctgtct ggctcataat attaatgagt actttggtat acaagaaaca aaatatattc
      181 ttgatcaatt agagatgaag tattctgatc tattaaaaga aatattaaga tatataaccg
      241 tgcagcgcat ttcagaggta attcagcgtc tgatccagga gcgaatttca gtgcgcaata
      301 tgcggttagt tatggaggcc ttagcattat ggagcccacg agagaaagat ataatcacac
      361 tcgtcgagca tgtacgaggt gcattaggtc gctacatttg ccataagttt tcatattctg
      421 gagaaattaa agcaattgtg atttcgcctg aaatagagga cagaatcaga gatggtgtca
      481 ggcctaccgc tggcggtact tttcttaatc tcgatgcatc cgaagctgag atgattctgg
      541 ataattttaa actggcactt tctggaatca atattcctat taaa
//
LOCUS       DBFIFI010000552          577 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.558, whole genome shotgun sequence.
ACCESSION   DBFIFI010000552 DBFIFI010000000
VERSION     DBFIFI010000552.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.558"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..>577)
                     /locus_tag="N7B39_005225"
     CDS             complement(<1..>577)
                     /locus_tag="N7B39_005225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HGU2424965.1"
                     /translation="LWPGFHNPVVLNSVTVGPQSTPYTMYFNFDKALVPFLLVLCTSS
                     LFKKEVKSEVSLWKWGALSLSVPLILFLAVFFGGLKPEIHFPEWLPEFILANLFFVSL
                     AEESLFRGYIQSRLSEVTSPLVALIVAALLFGFYHYSGGALLVLFATLSGVVYGLSWM
                     WSGRLWVATLFHFGLNLCHLLFFTYPFLKHN"
BASE COUNT          238 a          130 c           93 g          116 t
ORIGIN      
        1 caattatgtt ttaaaaatgg ataggtaaag aataacaagt gacacagatt caaaccaaaa
       61 tggaaaaggg tggcaaccca caaacgccca ctccacatcc atgacaatcc atacacaaca
      121 ccagataacg tggcaaataa tacaagtaaa gcaccacctg aatagtgata aaaaccaaac
      181 aacaaagccg ccacaattaa tgcaaccaat ggagacgtta cttctgatag ccgtgattga
      241 atataccctc taaataatga ttcctctgcc agagacacaa aaaacaaatt agccaatata
      301 aactctggca accactcagg aaaatgaatc tctggcttta atccaccaaa aaaaacagcc
      361 aaaaacagga taagaggaac agagagcgac agagcccccc acttccacaa agacacttct
      421 gattttactt cttttttaaa caaagaagat gtacacagga ctaacaaaaa tggcaccagc
      481 gctttatcaa aattaaaata cattgtatag ggagtacttt gagggccaac agtaacagaa
      541 tttagcacta caggattgtg aaaccctggc catagat
//
LOCUS       DBFIFI010000553          569 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.559, whole genome shotgun sequence.
ACCESSION   DBFIFI010000553 DBFIFI010000000
VERSION     DBFIFI010000553.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.559"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..301)
                     /locus_tag="N7B39_005226"
     CDS             complement(<1..301)
                     /locus_tag="N7B39_005226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EmrE family multidrug efflux SMR transporter"
                     /protein_id="HGU2424966.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGLFGQRLDLPAIIGMMLICAGVLV"
     gene            complement(369..>569)
                     /locus_tag="N7B39_005227"
     CDS             complement(369..>569)
                     /locus_tag="N7B39_005227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HGU2424967.1"
                     /translation="QAAAKMARAGILVVDGKVWRTVYYRFATREEREGKVSTNLIFKE
                     CRQSAAMKRVLRVYKRTSMGTQ"
BASE COUNT          170 a          156 c           98 g          145 t
ORIGIN      
        1 taaccaacac accggcacaa atcaacatca tgcctataat ggctggcagg tccagccgtt
       61 ggccgaaaag tccccatgac agtaagctaa tcaggacaat accgactcct gaccagatag
      121 cataagcaat ccctgtagga atataagcca gcgtctgagc taataaccag aatgatgcac
      181 aataacaaat aattgtacca acagatggcc ataaccgtgt aaaaccttct gaaaacttca
      241 ttaaggttgt accaatgacc tctgcaagta ttgcaccacc aagataaata taagggttca
      301 tagaatattc tttcctgttc aaactggaga gaattgtact acagtttgaa ctcaactcac
      361 ctgtttcatc attgtgtacc cattgatgtt cttttatata ccctcaatac ccgtttcatc
      421 gcggcactct ggcgacactc cttaaaaatc agattcgtgc tcacctttcc ttcccgttct
      481 tctctggtag cgaaccggta atacaccgtt cgccagacct taccatcaac gaccaggatt
      541 cctgcccgcg ccattttagc cgcagcctg
//
LOCUS       DBFIFI010000554          569 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.560, whole genome shotgun sequence.
ACCESSION   DBFIFI010000554 DBFIFI010000000
VERSION     DBFIFI010000554.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.560"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..>569)
                     /locus_tag="N7B39_005228"
     CDS             complement(<1..>569)
                     /locus_tag="N7B39_005228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HGU2424968.1"
                     /translation="GRYITQDPIGLKGGWNFYQYPLNPVINVDPQGLVDINLYPESDL
                     IHSVADEINIPGVFTIGGHGTPTSIESATRSIMTAKDLAYLIKFDGNYKDGMTVWLFS
                     CNTGKGQNSFASQLAKELHTNVIGPDTLWTWWGRGTNGKLKMDTVLTAPTNLNSNKDL
                     MAITTKDLGNWITYGPSGHPISNMQGTPE"
BASE COUNT          153 a          128 c          101 g          187 t
ORIGIN      
        1 ttttctggcg taccttgcat attagaaatg gggtgcccag atggcccata tgttatccaa
       61 ttaccaaggt cttttgttgt tatagccatt agatccttat ttgaattaag gttcgttggt
      121 gctgttagca ctgtatccat ttttaactta ccattagttc ctcgccccca ccacgtccat
      181 agcgtgtcag gtcctattac atttgtatgt aactctttag ctaattggct agcaaatgaa
      241 ttttgtcctt tacctgtatt acaagaaaat aaccaaactg tcatcccatc tttataattc
      301 ccatcaaatt taattagata tgctagatct ttagctgtca tgatactgcg cgttgcggat
      361 tcaatagatg tgggggtacc atgccccccg attgtgaaaa cgcctgggat attaatctca
      421 tcagctacag aatggataag atcactttcg gggtataaat ttatatcaac caaaccttgc
      481 ggatctacat ttatgaccgg attcaacgga tactgataaa aattccatcc ccccttcagt
      541 ccaatcggat cctgagtgat atatcgccc
//
LOCUS       DBFIFI010000555          564 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.561, whole genome shotgun sequence.
ACCESSION   DBFIFI010000555 DBFIFI010000000
VERSION     DBFIFI010000555.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.561"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            405..>564
                     /locus_tag="N7B39_005229"
     CDS             405..>564
                     /locus_tag="N7B39_005229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424969.1"
                     /translation="MSQNDIIIRTHYKSPHRMHIDSDIPTPSSEPINQFAPQLITLLD
                     TSDLSSMLS"
BASE COUNT          189 a          124 c           95 g          156 t
ORIGIN      
        1 aggtacacaa atgaaagaca ttaacctctc accttccacc cggttgagcg atgttaacac
       61 taaaaaaagg gagattgtac ctttccgttt cacattgatt cgattcgcaa ttcgtttgta
      121 gtgagtacac cagcaaaacg cttaaagaac aactgataac atcacggagc gtctgaaagg
      181 tttactgcga gcgaatttac agcagcataa actgaatcca gcaaagttcg ttgcgtgatt
      241 tggcggtact atttaaacga tcataagatt aatgacgaag tggtcatatc acaatgataa
      301 aagtgacaca attcttatag caatttttcg tgcacatttc gttctggcga taataattaa
      361 tcatcatact caaaacctcc agacatatat aaggcgaata gattatgagt caaaacgaca
      421 ttattatcag aactcattat aagtctcctc atagaatgca catcgatagc gacataccaa
      481 cgccttcatc agagcctatt aatcaatttg cgccccaact catcacccta cttgatacct
      541 ctgacttaag ttcgatgctg tcat
//
LOCUS       DBFIFI010000556          564 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.562, whole genome shotgun sequence.
ACCESSION   DBFIFI010000556 DBFIFI010000000
VERSION     DBFIFI010000556.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.562"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..494)
                     /locus_tag="N7B39_005230"
     CDS             complement(<1..494)
                     /locus_tag="N7B39_005230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HGU2424970.1"
                     /translation="MPGLISYVSSASFVNEMMELRQQVMEGQIGGFLLGGERVRVSYM
                     PDTGRFLAESEGQGRVYAELLNIAFNDGVNVLRNRILSALPGMGGRNSLQEKISECAF
                     TVDIEKLQCPGDALQCPITLEQPEKGVFVKNSDGSDVCTLFDAAAFSRLTGEDLPHPL
                     TREP"
BASE COUNT          170 a          158 c           89 g          147 t
ORIGIN      
        1 attggttccc gggtcagtgg gtggggtaag tcttcaccag tcaaacgaga aaatgcagcg
       61 gcatcaaata aagtacatac atctgaacca tctgaattct tcacaaaaac acctttttca
      121 ggctgctcca gtgtaattgg acattgaagc gcatcaccag gacactgaag tttttcaata
      181 tcgacagtaa aggcacactc cgatattttt tcctgcaaag agtttcgccc tcccattcca
      241 gggagcgcac ttaatatcct gtttctgagc acattaactc catcattaaa agcaatattc
      301 aataattctg cataaacccg tccctgccct tcactttctg ctaaaaaacg gcctgtatct
      361 ggcatataag aaactctaac cctctcccct cccaggagaa atccaccaat ctgcccctcc
      421 attacctgct gacgcagctc catcatctca ttcacgaatg aagccgatga cacatatgat
      481 attaatccag gcattaccat gctccttcat acggataaat attccctcaa gtcttcatta
      541 acaaacatcc accggacatg acaa
//
LOCUS       DBFIFI010000557          559 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.563, whole genome shotgun sequence.
ACCESSION   DBFIFI010000557 DBFIFI010000000
VERSION     DBFIFI010000557.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.563"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..>559
                     /locus_tag="N7B39_005231"
     CDS             <1..>559
                     /locus_tag="N7B39_005231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGU2424971.1"
                     /translation="KNFIIHGPQLISCSNNSMLIRCLRQDSSVEIECHDILMSAENAE
                     ELLVHRKADLVITQMPVISRSVICMPLHTIRNTLICSNRHPRITDNSTYEQIMAEEFT
                     QLISKSAGVDDIQMEIDERFMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSHRD
                     FLNLKEIKLEHPLPSIKLYISYNKSS"
BASE COUNT          173 a          120 c           96 g          170 t
ORIGIN      
        1 gaaaaacttt attattcacg gccctcagct catttcttgc tctaataata gtatgctgat
       61 ccgctgccta cggcaagatt cctctgtcga gattgagtgc catgatatcc tcatgtcagc
      121 agagaatgct gaagaactgt tggttcaccg caaagccgat ctggtgatca cccaaatgcc
      181 agtgatcagc cgttccgtca tttgcatgcc attgcatacg attcgcaata ctctaatttg
      241 tagtaatagg catccacgaa ttactgacaa tagcacttat gaacaaatta tggcagaaga
      301 gttcacccaa ttaatatcta aatcagcagg ggttgatgat attcaaatgg aaatagacga
      361 gagatttatg aatcgcaaaa tttcatttcg tggttcctct ttgctgacaa tcattaatag
      421 cattgcagtt acagatttac tgggaatagt cccctatgaa ctttataact cccaccgtga
      481 ctttcttaat ttaaaagaga taaaactaga acatccgtta ccttcaataa agctttacat
      541 ttcctataat aaatcttct
//
LOCUS       DBFIFI010000558          558 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.564, whole genome shotgun sequence.
ACCESSION   DBFIFI010000558 DBFIFI010000000
VERSION     DBFIFI010000558.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.564"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            3..>558
                     /locus_tag="N7B39_005232"
     CDS             3..>558
                     /locus_tag="N7B39_005232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc26 family transposase"
                     /protein_id="HGU2424972.1"
                     /translation="MSVIPDIRQQGKVKHKLSDILFLTVCAVIAGADEWQEIEDFGHE
                     RLEWLKKYGDFDNGIPVDDTIARVVSNIDSLAFEKIFIEWMQECHEITDGEIIAIDGK
                     TIRGSFDKGKRKGAIHMVSAFSNENGVVLGQVKTEAKSNEITAIPELLNLLDLKKNLI
                     TIDAMGCQKDIASKIKDKKADYLLA"
BASE COUNT          194 a           80 c          134 g          150 t
ORIGIN      
        1 atatttcagt gatccctgat atacgacaac aaggaaaggt taaacataaa ttatctgata
       61 ttttgtttct caccgtatgt gcagtaattg caggtgccga tgagtggcag gaaattgaag
      121 attttggaca tgaaagactt gaatggctaa agaaatatgg tgattttgat aatggcattc
      181 cggtcgatga caccattgca cgcgttgtga gtaacattga cagtttggcc tttgaaaaga
      241 tatttattga atggatgcag gagtgccatg aaatcactga tggtgaaatt atagcaatag
      301 atggaaagac cataagaggc tcctttgata agggaaaaag aaaaggagca atccatatgg
      361 tgagtgcatt ctcgaacgaa aatggtgttg tactggggca ggtaaaaacg gaagccaaaa
      421 gtaatgagat tacagccatt ccagagttgc ttaacctact ggatttaaag aaaaatttga
      481 taaccattga tgctatgggc tgtcagaaag atatcgcttc gaagatcaaa gataaaaaag
      541 cagattatct tctggcag
//
LOCUS       DBFIFI010000559          557 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.565, whole genome shotgun sequence.
ACCESSION   DBFIFI010000559 DBFIFI010000000
VERSION     DBFIFI010000559.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.565"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..>557
                     /locus_tag="N7B39_005233"
     CDS             <1..>557
                     /locus_tag="N7B39_005233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU2424973.1"
                     /translation="ESLGFRLKRLRKDKGLTQVELGKLSGVTGVTIGYWEKDLNEPGS
                     KALSKLAQALGTTESYLLYGVSSPELSFVQSTSGTKIPYLSWGEAISFLILKGEKTMG
                     NVDRITTFFDVEEGDFAVSMPDDTMHNPSGSPSIPVGATVILRPGESYKNGSIVAVIV
                     PDPLKNEPSMTIKKLVIDGKLVYLSP"
BASE COUNT          170 a          109 c          132 g          146 t
ORIGIN      
        1 gaaagccttg gcttcaggct taagagactt agaaaagata aggggctgac ccaagtagaa
       61 ctgggtaagc tttcaggcgt gactggggtt actatagggt actgggagaa agatctaaac
      121 gaaccaggta gcaaagctct aagtaagtta gcccaggcat taggaactac tgagtcctat
      181 ctcctatatg gagtatcgtc tcctgaatta tcttttgtac aaagtacctc aggcaccaag
      241 atcccctacc tttcgtgggg tgaggcgatt tctttcctaa tcttaaaagg agagaaaaca
      301 atgggaaatg tcgatagaat caccacattc tttgatgtcg aggaaggtga ttttgccgtt
      361 tcaatgcctg atgacacaat gcataaccca tcaggatcac cgagtatccc agttggtgct
      421 actgtgatcc taaggccagg agaaagttat aaaaatggca gcatcgtcgc tgtaatagtt
      481 ccggatccgc ttaaaaatga accatctatg actataaaga aattagttat tgatgggaag
      541 cttgtgtatt taagccc
//
LOCUS       DBFIFI010000560          549 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.566, whole genome shotgun sequence.
ACCESSION   DBFIFI010000560 DBFIFI010000000
VERSION     DBFIFI010000560.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.566"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(63..139)
                     /locus_tag="N7B39_005234"
     tRNA            complement(63..139)
                     /locus_tag="N7B39_005234"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(103..105),aa:Arg,seq:tcg)
     gene            complement(147..222)
                     /locus_tag="N7B39_005235"
     tRNA            complement(147..222)
                     /locus_tag="N7B39_005235"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(187..189),aa:Ile,seq:cat)
     gene            complement(251..>549)
                     /locus_tag="N7B39_005236"
     CDS             complement(251..>549)
                     /locus_tag="N7B39_005236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU2424974.1"
                     /translation="IIQTDISASWTLGAIALRLHMSESLLKIKLKNEGHMFSRLLLEE
                     RMRVAVNMLCSRHGYGQAVAEKCGYSSWSYFISVFHRYYGFPPDRYVSRQGLDY"
BASE COUNT          153 a          140 c          122 g          134 t
ORIGIN      
        1 gaccgattgc ttagaaggca attgctctgt ccggctgagc taacaacgca ggatacagat
       61 aatggaccgc cttcggggac ccgaactccg cgcaaccagc ttcgaaagct ggcgctcttt
      121 cctgatgagc taatggcggt atgtgatggt ggcccttgct ggatttgaac cagcgacctg
      181 gcgattatga gtcgctcgct ctcaccactg agctaaaggg ctgggtcaaa aaataataat
      241 cagatgaaaa tcaataatca agcccttgcc tggatacata tctgtctggc gggaagccat
      301 aatagcggtg aaatacagaa ataaagtagg accagcttga ataaccgcat ttttctgcta
      361 cagcctgtcc atatccatgc cgggaacata acatattgac agcaacacgc atccgctctt
      421 ccagcaacaa gcgactgaac atgtgccctt catttttcag ttttatcttt aacaaactct
      481 cactcatatg caggcgtaac gcaatcgcac caagcgtcca gcttgctgat atatctgtct
      541 gaattatcg
//
LOCUS       DBFIFI010000561          537 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.567, whole genome shotgun sequence.
ACCESSION   DBFIFI010000561 DBFIFI010000000
VERSION     DBFIFI010000561.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.567"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            30..449
                     /locus_tag="N7B39_005237"
     CDS             30..449
                     /locus_tag="N7B39_005237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424975.1"
                     /translation="MKLFHGTYEKTVPIIKPGEFVMSGNNVFDGLFSSPERDIAASHG
                     NPVFIYHVDDDKIAKSRDLDARFQEVYAFLHNELDTADVEEIADRVMWDNNSDIEDFA
                     DILSPRLGSDCSILVCLYANSFGFIYAQRSQQFIWYF"
BASE COUNT          157 a           98 c           94 g          188 t
ORIGIN      
        1 ttacataaaa taaatacagt atcgttatta tgaaattatt tcacggcact tatgaaaaaa
       61 cagtaccaat aatcaaacct ggtgagttcg taatgtctgg caataacgtt tttgatgggt
      121 tgttttccag cccggagcgc gatattgctg ccagtcacgg aaaccccgta ttcatatacc
      181 atgttgatga tgataaaata gctaaatccc gcgatcttga tgctcgtttt caagaggttt
      241 atgccttctt acataacgaa ctggatactg ctgacgtaga ggaaatagct gatcgcgtca
      301 tgtgggataa caattcagat atcgaagatt tcgcagatat tctctcgcca cgcctcggca
      361 gcgattgttc tattcttgtt tgtttatacg caaactcgtt tgggtttatt tatgctcaga
      421 gaagtcagca attcatctgg tatttttgaa ttactttttt gataaataac attttcatat
      481 attttaaaat catcatattt aaatttttta atctccttaa aacttctttc attgcag
//
LOCUS       DBFIFI010000562          531 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.568, whole genome shotgun sequence.
ACCESSION   DBFIFI010000562 DBFIFI010000000
VERSION     DBFIFI010000562.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.568"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            69..>531
                     /locus_tag="N7B39_005238"
     CDS             69..>531
                     /locus_tag="N7B39_005238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HGU2424976.1"
                     /translation="MLWTENDAENTSQWNGYPLQIGRFRKDKAMPALISGEKSTALVT
                     PPQWRNKAFNGLKDPERNYWAKEQITGSPEENIKAAITYLMMKLSNTKEESTIDQYDS
                     TLYSAIVQKGDLADNIRKERKTTIPNLTKNNPGKNLDKIHPGDILYYQKASM"
BASE COUNT          209 a          105 c           90 g          127 t
ORIGIN      
        1 atgaaatcaa cattcatttg tccagcactc catactttca gccgttggat tggtatataa
       61 taaaagccat gctctggact gaaaacgatg cggaaaatac ttctcagtgg aatggttatc
      121 cattacaaat tggtcgtttt cggaaagata aagcgatgcc agctttaatc tctggagaaa
      181 aatctacagc ccttgttacc ccaccacaat ggagaaacaa agcattcaat gggttaaaag
      241 atccagaaag aaattactgg gcaaaagaac aaataactgg ctctccagaa gagaatatca
      301 aagcagcaat tacttactta atgatgaaac tttctaatac aaaggaagaa tcaactatcg
      361 atcaatatga ctcaactcta tatagcgcca ttgtccaaaa aggcgatttg gctgacaaca
      421 tcagaaaaga gagaaaaacg accataccta acttaacgaa aaacaatcct ggaaaaaatc
      481 tcgataaaat acacccggga gatattttat attatcaaaa agcatccatg a
//
LOCUS       DBFIFI010000563          530 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.569, whole genome shotgun sequence.
ACCESSION   DBFIFI010000563 DBFIFI010000000
VERSION     DBFIFI010000563.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.569"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
BASE COUNT          138 a           83 c          110 g          199 t
ORIGIN      
        1 atggctctgt tacagtttat aggtatgaaa aaatataatc tttcctgtat gaaataatat
       61 tcttatggtg tgtggggtat ggtctgtata ccatcatttt tatggtgaag ttaaatatct
      121 gtagaggcct taagctgggt cgctgttttt acttaatggt acagaagtat taatagcggt
      181 gttgcctgtg gctacccgat actggctttt gtgaatgtca gattacttcc tgtaagtgat
      241 tgaatggtac ttccttaaac tcaattctat gaggtgtgtt tttaccattg taattcgtac
      301 tggtcctcag tttaagtgag tcttactgta ctctgaaata ttacctcttt ggagtaatga
      361 ggtaatacga aacagtttta tcaggttgtc tgtgctgctg gttacctcgt ttattaatgc
      421 cgcggtacgg cgtttctgta ctctcagtat ttatgtaatt ccaccttcct gtaactttta
      481 taaagtaagg actataaaat gtataatgct aatcccaatt acgaaatgga
//
LOCUS       DBFIFI010000564          528 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.570, whole genome shotgun sequence.
ACCESSION   DBFIFI010000564 DBFIFI010000000
VERSION     DBFIFI010000564.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.570"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(<1..218)
                     /gene="escT"
                     /locus_tag="N7B39_005239"
     CDS             complement(<1..218)
                     /gene="escT"
                     /locus_tag="N7B39_005239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscT"
                     /protein_id="HGU2424977.1"
                     /translation="MNEIMTVIVSSFYCILRPLGMFIILPIFSTGVLLSNFIRNSIMI
                     AFTLPIIVENYTFSEKLPSGIFQLTGIAL"
     gene            complement(218..487)
                     /gene="escS"
                     /locus_tag="N7B39_005240"
     CDS             complement(218..487)
                     /gene="escS"
                     /locus_tag="N7B39_005240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscS"
                     /protein_id="HGU2424978.1"
                     /translation="MDTGYFVQLCVQTFWIIFILSLPTVIAASVIGIIISLVQAITQL
                     QDQTLPFLLKIIAVFATLALTYHWMGTTIINFSSIIFEMIPKVNG"
BASE COUNT          182 a          104 c           95 g          147 t
ORIGIN      
        1 agcgctattc ctgtcaactg aaagattcca gatggcaatt tttccgaaaa tgtatagttc
       61 tctactatga tcggaagcgt aaacgcaatc ataattgagt tgcgaataaa gttactgagt
      121 aaaacacctg ttgaaaatat tggtaggata ataaacatcc ccagcggcct gagtatacaa
      181 taaaatgatg atactatgac cgtcattatc tcattcatta gccgttcacc ttcggaatca
      241 tttcaaatat tatcgacgag aagttgatga ttgttgttcc catccagtga taagtcaggg
      301 caagcgtagc aaacactgct attattttta gcaaaaaagg caatgtttga tcctgtaact
      361 gcgttatagc ctggaccaga ctaataataa taccgataac agaggccgct atgactgtag
      421 gcaatgagag gataaatatt atccagaacg tttgcacaca taattgaaca aaatatccag
      481 tatccatcaa ttcaccacca acagaaattc gaataatttt tgccagcc
//
LOCUS       DBFIFI010000565          527 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.571, whole genome shotgun sequence.
ACCESSION   DBFIFI010000565 DBFIFI010000000
VERSION     DBFIFI010000565.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.571"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..>527
                     /locus_tag="N7B39_005241"
     CDS             <1..>527
                     /locus_tag="N7B39_005241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424979.1"
                     /translation="VEMLKLNMICTWDLRSSVYYLTMKMLGQPGTPEYAKYSELSASI
                     FNELSDASDTKGYWSTDVKLVSSSGHEYTEREFELEREKSTRGKGGMTRSLEMFIASL
                     NWLAYKSIYYSVIAKHFKADSFIHPIRHAYQLHWMKKTGAFGHDYTARLVQSLSDKIS
                     TARSEIVDHGRTSTIS"
BASE COUNT          165 a           85 c          114 g          163 t
ORIGIN      
        1 gtagagatgc ttaagctgaa tatgatttgc acatgggatc ttcggtctag cgtgtattac
       61 ttaacaatga aaatgcttgg tcagcccggt actcctgaat atgcgaaata tagtgaacta
      121 agtgcatcga tttttaatga attatcagat gcttctgata cgaaagggta ctggtcaacc
      181 gatgtgaagc tagtgagttc atctggacat gaatatactg agcgtgaatt tgaactagaa
      241 agggaaaaat ctactcgcgg taaaggaggt atgactcgtt cacttgaaat gtttatcgca
      301 tcgctaaatt ggttggcata taaatctatt tattacagcg tcattgctaa gcattttaaa
      361 gcagactcat ttattcatcc aataaggcat gcatatcaat tgcattggat gaaaaaaaca
      421 ggtgcttttg gtcatgatta tactgcaagg ttggtgcaat cgttgtctga taaaatcagt
      481 actgcaagat ctgaaattgt agatcatgga cgcacatcta ctatttc
//
LOCUS       DBFIFI010000566          524 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.572, whole genome shotgun sequence.
ACCESSION   DBFIFI010000566 DBFIFI010000000
VERSION     DBFIFI010000566.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.572"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..359
                     /locus_tag="N7B39_005242"
     CDS             <1..359
                     /locus_tag="N7B39_005242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="secretion protein EspS"
                     /protein_id="HGU2424980.1"
                     /translation="HEIDDIRLTSCNSANIIKNKDFSPDEIEKSANMNNGWLARALFG
                     QKRSLAEHVYAEFERRGINVSISGYHGTGVFYVPEHGKPTTHLRSTTVPATPEHTVRR
                     SDYRATLGRTQPIDIE"
BASE COUNT          170 a          111 c          110 g          133 t
ORIGIN      
        1 ttcatgaaat agatgatatc agattaacat cctgtaactc agccaacata ataaaaaaca
       61 aagacttctc tcctgatgaa atagaaaaat ccgcaaatat gaataacggc tggttggcca
      121 gggcattatt tggtcaaaag aggtctttag cagaacacgt ctatgccgag tttgaacgtc
      181 gcggaattaa cgtttctata tcaggttacc atggcactgg cgttttttat gtaccagagc
      241 atggtaaacc aacaacgcat ctacgctcca caactgtgcc tgcaacacct gaacatactg
      301 taagaagaag cgactacaga gccactttgg gtagaactca acccatagat attgagtaat
      361 tgagccagca tatacacttg atggttagga aacagaatca ttgttttaaa catttcctgg
      421 ccattaatat tgtcggcggg agcatatcca ggactggccg gcaaaccggt acgcgatctg
      481 ttgccatgga aagttgatct gagcgctcag taaatatcaa tacg
//
LOCUS       DBFIFI010000567          521 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.573, whole genome shotgun sequence.
ACCESSION   DBFIFI010000567 DBFIFI010000000
VERSION     DBFIFI010000567.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.573"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            22..296
                     /locus_tag="N7B39_005243"
                     /pseudo
     CDS             22..296
                     /locus_tag="N7B39_005243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309594.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspO"
     gene            complement(376..>521)
                     /locus_tag="N7B39_005244"
     CDS             complement(376..>521)
                     /locus_tag="N7B39_005244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424981.1"
                     /translation="RHLHRIVYRVKYFNFTTLHDYNHFCDFIEFKHKNIIMNTSQYTG
                     SSW"
BASE COUNT          202 a          115 c           84 g          120 t
ORIGIN      
        1 catggagcaa aggagaaaac aatgccattt tcaatcaaaa acagattttc aagttcacaa
       61 gtacattacc cggaaatatc cggtcccata aaagacaagc cagcgtcaaa gaactgcata
      121 cttacatcaa caacatgtaa tgtagatagc tatacagtgt accaaaaaaa gcctgtagtt
      181 ttgacatgcg cccacccggc gcaggagaaa gaaccccaaa actaaaactc tcagttactg
      241 agatgacatg gctatctaaa actatagaaa cagagataca caacacaaaa gaatagcaac
      301 cacttaccag agaacacaaa aagccacaga cccgaaacac ctgactgcaa gcaaccacct
      361 ccacaggagg tggttttacc atgaacttcc tgtgtactga cttgtgttca taataatatt
      421 tttgtgttta aactcaataa agtcacaaaa atgattgtaa tcatgcaatg tagtaaaatt
      481 aaaatatttg accctgtaca cgattctgtg taaatgcctt t
//
LOCUS       DBFIFI010000568          519 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.574, whole genome shotgun sequence.
ACCESSION   DBFIFI010000568 DBFIFI010000000
VERSION     DBFIFI010000568.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.574"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..>519
                     /locus_tag="N7B39_005245"
     CDS             <1..>519
                     /locus_tag="N7B39_005245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HGU2424982.1"
                     /translation="ECRHFALQYCIDSMNEHTGKVPLKAYYSSPEDIQKHIPFELEQQ
                     FNNLQKNPPPGTCVVASDKFGEALSVFFHRMEKEKLTHMTAIVQSQTHAMAVRLRIKK
                     TPAGETEYVVSFYDPNATNTAVRYKANNCDSFGSLQSFINIQQAKQKWVITDICSECV
                     GITPYLPREQAHL"
BASE COUNT          174 a          106 c           97 g          142 t
ORIGIN      
        1 gaatgtagac attttgctct gcaatactgt atagattcaa tgaatgagca tacagggaaa
       61 gttccattaa aggcttacta ttcatctccg gaagatatac aaaaacatat tcctttcgag
      121 cttgagcagc aatttaacaa tctacaaaaa aatccaccac ccggtacatg cgtcgttgcc
      181 agtgataagt ttggggaggc attatctgtc ttttttcaca gaatggaaaa agagaagtta
      241 acgcatatga cggcaatcgt tcaatctcaa acacacgcta tggccgtccg cttgaggatc
      301 aaaaaaacgc ctgctggtga aacagaatat gttgtatcct tttatgatcc taatgcaacc
      361 aatactgcag tacgctataa agcaaacaac tgtgattctt ttgggtcatt gcaatcgttt
      421 ataaatattc aacaggcaaa acaaaaatgg gtaataacag atatttgctc cgagtgtgta
      481 ggaataaccc cttatctccc tcgggaacaa gcccattta
//
LOCUS       DBFIFI010000569          519 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.575, whole genome shotgun sequence.
ACCESSION   DBFIFI010000569 DBFIFI010000000
VERSION     DBFIFI010000569.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.575"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            243..>519
                     /locus_tag="N7B39_005246"
     CDS             243..>519
                     /locus_tag="N7B39_005246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HGU2424983.1"
                     /translation="MDCSKCNGYATMLLNMVQGSDPVNLLELHGFLEHFAYYVSFGKF
                     NAGHQRYNAFKKFVSEISEISANDINMTIKTGQSRHENVISINMNDAI"
BASE COUNT          163 a           90 c          102 g          164 t
ORIGIN      
        1 tgcagtcgtt tcattaccgt atatctttag tagttctaat aaactatcaa aaatcgctca
       61 tttcagctta ttgatagaaa gattgattgc cagtctgttt agagccgtac agcagcaaac
      121 tttcataacc aggttgatcc tgaatttgca gtacgaaaag ttaaaaatga gcgatttctg
      181 taagactccc cattttgcca ggcttatgat tcgcagatca ttcaagtgca aaggagcgat
      241 gtatggattg ttcaaaatgc aatggttatg cgacgatgtt attaaatatg gtgcagggtt
      301 ctgacccggt taatttactg gaattacatg gtttccttga acatttcgca tattatgttt
      361 catttggcaa atttaatgct ggccaccagc gctataatgc atttaaaaaa ttcgtcagtg
      421 agatttcgga gatcagcgca aatgatatca atatgacgat taaaaccggg caatccagac
      481 atgaaaatgt gatctctata aatatgaatg atgcaattc
//
LOCUS       DBFIFI010000570          513 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.denovo.576, whole genome shotgun sequence.
ACCESSION   DBFIFI010000570 DBFIFI010000000
VERSION     DBFIFI010000570.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.denovo.576"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            complement(279..413)
                     /locus_tag="N7B39_005247"
     CDS             complement(279..413)
                     /locus_tag="N7B39_005247"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2424984.1"
                     /translation="MPHPERNHCLLLMGYLHGVQASELLGIKLSDIDLQAGNLNIRRL
                     "
BASE COUNT          139 a          135 c          104 g          135 t
ORIGIN      
        1 gcggaatttt ctgcatccgg ataccactcc ccctgaaaaa tactcgcaca atatcgcgcc
       61 gcatgcactg atggaccggg ccatcgtgag aatctcttgc atcgtagata ttaaccacac
      121 cagtgccccc cagaacaaca gtaatacctg tagcagacct gccatagagg ttaaataacc
      181 attcatatcc ctcttctacc cattcatccc gaagtttcag ccagcgacga atggactgta
      241 cctcctgttt cagccgaggg gctgagttga caaaccgttt agagccgccg gatattcagg
      301 ttaccggcct gcaaatcaat atcagacagt tttataccta ataactctga tgcttggaca
      361 ccatgaagat accccataag gagcaggcag tgattacgtt ccgggtgtgg cactttgaag
      421 acagcactgt gcaacttatg aatctcaccc gggttcagat atttttgttg tccattacct
      481 gtccttattt attgaaagtc gatattagtt taa
//
LOCUS       DBFIFI010000571         3960 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.guided.287, whole genome shotgun sequence.
ACCESSION   DBFIFI010000571 DBFIFI010000000
VERSION     DBFIFI010000571.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3960
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.guided.287"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..204
                     /locus_tag="N7B39_005248"
     CDS             <1..204
                     /locus_tag="N7B39_005248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313162.1"
                     /note="part of four member fumarate reductase enzyme
                     complex FrdABCD which catalyzes the reduction of fumarate
                     to succinate during anaerobic respiration; FrdAB are the
                     catalytic subcomplex consisting of a flavoprotein subunit
                     and an iron-sulfur subunit, respectively; FrdCD are the
                     membrane components which interact with quinone and are
                     involved in electron transfer; the catalytic subunits are
                     similar to succinate dehydrogenase SdhAB; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     flavoprotein subunit"
                     /protein_id="HGU2424985.1"
                     /translation="LDEGCTKRDDVNFLKHTLAFRDADGTTRLEYSDVKITTLPPAKR
                     VYGGEADAADKAEAANKKEKANG"
     gene            197..931
                     /gene="frdB"
                     /locus_tag="N7B39_005249"
     CDS             197..931
                     /gene="frdB"
                     /locus_tag="N7B39_005249"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HGU2424986.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            942..1337
                     /gene="frdC"
                     /locus_tag="N7B39_005250"
     CDS             942..1337
                     /gene="frdC"
                     /locus_tag="N7B39_005250"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HGU2424987.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            1348..1707
                     /gene="frdD"
                     /locus_tag="N7B39_005251"
     CDS             1348..1707
                     /gene="frdD"
                     /locus_tag="N7B39_005251"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HGU2424988.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            1770..2903
                     /locus_tag="N7B39_005252"
     CDS             1770..2903
                     /locus_tag="N7B39_005252"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HGU2424989.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIVNGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            2991..3524
                     /gene="blc"
                     /locus_tag="N7B39_005253"
     CDS             2991..3524
                     /gene="blc"
                     /locus_tag="N7B39_005253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HGU2424990.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(3521..3838)
                     /gene="sugE"
                     /locus_tag="N7B39_005254"
     CDS             complement(3521..3838)
                     /gene="sugE"
                     /locus_tag="N7B39_005254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HGU2424991.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
BASE COUNT          976 a         1033 c         1038 g          913 t
ORIGIN      
        1 ctggacgaag gttgcaccaa gcgtgacgac gtcaacttcc tcaaacacac cctcgccttc
       61 cgcgatgctg atggcacgac tcgcctggag tacagcgacg tgaagattac tacgctaccg
      121 ccagctaaac gcgtttacgg tggcgaagcg gatgcagccg ataaggcgga agcagccaat
      181 aagaaggaga aggcgaatgg ctgagatgaa aaacctgaaa attgaggtgg tgcgctataa
      241 cccggaagtc gataccgcac cgcatagcgc attctatgaa gtgccttatg acgcaactac
      301 ctcattactg gatgcgctgg gctacatcaa agacaacctg gcaccggacc tgagctaccg
      361 ctggtcctgc cgtatggcga tttgtggttc ctgcggcatg atggttaaca acgtgccaaa
      421 actggcatgt aaaaccttcc tgcgtgatta caccgacggt atgaaggttg aagcgttagc
      481 taacttcccg attgaacgcg atctggtggt cgatatgacc cacttcatcg aaagtctgga
      541 agcgatcaaa ccgtacatca tcggcaactc ccgcaccgcg gatcagggta ctaacatcca
      601 gaccccggcg cagatggcga agtatcacca gttctccggt tgcatcaact gtggtctgtg
      661 ctacgccgcg tgcccgcagt ttggcctgaa cccagagttc atcggtccgg ctgccattac
      721 gctggcgcat cgttataacg aagatagccg cgaccacggt aagaaggagc gtatggcgca
      781 gttgaacagc cagaacggcg tatggagctg tactttcgtg ggctactgct ccgaagtctg
      841 cccgaaacac gtcgatccgg ctgcggccat tcaacagggc aaagtagaaa gttcgaaaga
      901 ctttcttatc gcgaccctga aaccacgcta aggagtgcaa catgacgact aaacgtaaac
      961 cgtatgtacg gccaatgacg tccacctggt ggaaaaaatt gccgttttat cgcttttaca
     1021 tgctgcgcga aggcacggcg gttccggctg tgtggttcag cattgaactg attttcgggc
     1081 tgtttgccct gaaaaatggc ccggaagcct gggcgggatt cgtcgacttt ttacaaaacc
     1141 cggttattgt gatcattaac ctgatcactc tggcggcagc tctgctgcac accaaaacct
     1201 ggtttgaact ggcaccgaaa gcggccaata tcattgtaaa agacgaaaaa atgggaccag
     1261 agccaattat caaaagtctc tgggcggtaa ctgtggttgc caccatcgta atcctgtttg
     1321 ttgccctgta ctggtaagga gcctgagatg attaatccaa atccaaagcg ttctgacgaa
     1381 ccggtattct ggggcctctt cggggccggt ggtatgtgga gcgcaattat tgcgccggtg
     1441 atgatcctgc tggtgggtat tctgctgccg ctggggctgt ttccgggtga tgcactgagc
     1501 tacgagcgcg ttctggcgtt cgcgcagagc ttcattggtc gcgtattcct gttcctgatg
     1561 atcgttctgc cgctgtggtg tggtttacac cgtatgcacc acgcgatgca cgatctgaaa
     1621 atccacgtac ctgcgggcaa atgggttttc tacggtctgg ctgctatcct gacagttgtc
     1681 acgctgattg gtgtcgttac aatctaacgc atcgccaatg taaatccggc ccgcctatgg
     1741 cgggccgttt tgtatggaaa ccagacccta tgttcaaaac gacgctctgc gccttattaa
     1801 ttaccgcctc ttgctccaca tttgctgccc ctcaacaaat caacgatatt gtgcatcgca
     1861 caattacccc gcttatagag caacaaaaga tccccggtat ggcggtggcg gtaatttatc
     1921 agggtaaacc ttattacttt acctgggggt atgcggacat cgccaaaaag cagcccgtca
     1981 cacagcaaac gttgtttgag ttaggttcgg tcagcaaaac atttacgggc gtgcttggtg
     2041 gcgacgctat tgctcgaggg gaaatcaagt taagcgatcc cgcaacaaaa tactggcctg
     2101 aacttaccgc taaacagtgg aatgggatca cactattaca tctcgcaacc tacactgctg
     2161 gcggcctgcc attgcaggtg ccggatgaag tgaaatcctc aagcgacttg ctgcgcttct
     2221 atcaaaactg gcagcctgca tgggctccag gaacacaacg tctgtatgcc aactccagta
     2281 tcggtttgtt cggcgcactg gctgtgaagc cgtctggttt gagttttgag caggcgatgc
     2341 aaactcgtgt cttccagcca ctcaaactca accatacgtg gattaatgta ccgcccgcag
     2401 aagaaaagaa ttacgcctgg ggatatcgcg aaggcaaggc agttcatgtt tcgccagggg
     2461 cgttagatgc tgaaacttat ggtgtgaagt cgaccattga agatatggcc tgctgggtac
     2521 gaagcaatat gaatccccgt gatatcaacg acaaaacact tcagcaaggg atacaactgg
     2581 cacaatctcg ctactggcaa accggcgata tgtatcaggg tctgggctgg gaaatgctgg
     2641 actggccggt aaatcctgac atcatcgtta acggcagtga caataaaatt gcactggcag
     2701 cacgccccgt aaaagcgatt acgcctccaa ctcctgcagt acgagcatca tgggtgcata
     2761 aaacgggggc gaccggcgga tttggtagct atgtcgcgtt tattccagaa aaagagctgg
     2821 gtatcgtgat gctggctaac aaaaactatc ccaatccagc gagagtcgcc gccgcctggc
     2881 agattctcaa cgctctacag taaaatttca tcgggtccga attttcggac cttttctgcg
     2941 cttttccttg ctgtcatcta cacttagaaa aaaccagtaa ggaaacattt atgcgcctgc
     3001 tccctctcgt tgccgcagcg acagctgcat ttctggtcgt tgcctgcagt tctcctacgc
     3061 cgccgcgtgg cgtgaccgta gtgaataatt tcgacgctaa acgctatctc ggtacctggt
     3121 atgagattgc ccgttttgat caccgctttg aacgtggact ggaaaaagtc accgcaacat
     3181 acagcctgcg tgatgacggc ggcctgaatg tcattaataa aggctataac cctgacagag
     3241 gaatgtggca gcagagtgaa gggaaagcgt actttaccgg cgcaccaact cgcgctgcgc
     3301 tgaaagtgtc attctttggt cctttctatg gcggttataa cgttattgca ctcgatcggg
     3361 aataccgcca tgcgctggtt tgcggaccgg accgcgacta cctgtggata ctctcccgca
     3421 cgccaaccat ttctgacgaa gtgaaacagg agatgctggc agtcgcgacc cgggaagggt
     3481 ttgatgtcag taagtttatt tgggtacagc agcccggtag ttagtgagtg ctgagtttca
     3541 gaccaataat ccccaatacg attaacgcca gactcgccag acgcatcggg ttagcggact
     3601 caccgagcag cacaatgccg gtgatggccg cgccgactgc gccaataccc gtccacacgg
     3661 cataagccgt ccctactggt aacgatttca tcgcccaggc aagtagcgcc atactgacaa
     3721 tcatcgccgt cacggtaata acactcggcg tcaaacgact aaagccgtgg gtatatttca
     3781 ggcctacggc ccataccact tccagcagac cagcaataac taagataatc caggacatat
     3841 caggctccgg agaattgggg ccgtccccgg tgaaaagacg cgtttgcagg tcgtcctgca
     3901 aagctgatgg ttgaacggat attttgccag gtggcggggg aatttcaact attacctttg
//
LOCUS       DBFIFI010000572         3276 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.guided.315, whole genome shotgun sequence.
ACCESSION   DBFIFI010000572 DBFIFI010000000
VERSION     DBFIFI010000572.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.guided.315"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            273..731
                     /gene="gpt"
                     /locus_tag="N7B39_005255"
     CDS             273..731
                     /gene="gpt"
                     /locus_tag="N7B39_005255"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HGU2424992.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            823..2067
                     /gene="frsA"
                     /locus_tag="N7B39_005256"
     CDS             823..2067
                     /gene="frsA"
                     /locus_tag="N7B39_005256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HGU2424993.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSCLLHQRVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            2125..2526
                     /gene="crl"
                     /locus_tag="N7B39_005257"
     CDS             2125..2526
                     /gene="crl"
                     /locus_tag="N7B39_005257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HGU2424994.1"
                     /translation="MTLPSGHPKSRLIKKFTVLGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(2565..>3276)
                     /locus_tag="N7B39_005258"
     CDS             complement(2565..>3276)
                     /locus_tag="N7B39_005258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HGU2424995.1"
                     /translation="LGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDF
                     FGVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQ
                     NLQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQ
                     FDFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDN
                     KLNINNDDIVAVGMTYQF"
BASE COUNT          795 a          792 c          846 g          843 t
ORIGIN      
        1 cagttcagac acgttaagtc tccttgtcga tcacccgcaa aacagtattg caggtcacag
       61 ggttagcaga aaatgttgtc aacacaagac aggcttgcga gatatgtttg agaataccac
      121 tttatcccgc gtcagggaga ggcagtgcgt aaaaagacgc ggactcatgt gaaatactgg
      181 tttttagtgc gccagatctc tataatctcg cgcaacctat tttcccctcg aacacttttt
      241 aagccgtaga taaacaggct gggacacttc acatgagcga aaaatacatc gtcacctggg
      301 acatgttgca gatccatgca cgtaaactcg caagccgact gatgccttct gaacaatgga
      361 aaggcattat tgccgtaagc cgtggcggtc tggtaccggg tgcgttactg gcgcgtgaac
      421 tgggtattcg tcatgtcgat accgtttgta tttccagcta cgatcacgac aaccagcgcg
      481 agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg cttcatcgtt attgatgacc
      541 tggtggatac cggtggtact gcggttgcga ttcgtgaaat gtatccaaaa gcgcactttg
      601 tcaccatctt tgcaaaaccg gctggtcgtc cgctggttga tgattatgtt gttgatatcc
      661 cgcaagatac ctggatcgaa cagccgtggg atatgggcgt cgtattcgtc ccgccaatct
      721 ccggtcgcta atcttttcaa cgcctggcac tgccgggcgt tgttcttttt aacttcaggc
      781 gagttacaat agtttccagt aagtattctg gaggctgcat ccatgacaca ggcaaacctg
      841 agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa cctcgacgct agtccgccgc
      901 tttaatcacg gcgcacaacc gcctgtgcag tcggcccttg atggtaaaac catccctcac
      961 tggtatcgca tgattaaccg tctgatgtgg atctggcgcg gcattgaccc acgcgaaatc
     1021 ctcgacgtcc aggcacgtat tgtgatgagc gatgccgaac gtaccgacga tgatttatac
     1081 gatacggtga ttggctaccg tggcggcaac tggatttatg agtgggccac ccaggcgatg
     1141 gtgtggcaac aaaaagcctg tgcggaagaa gatccgcaac tcagtggtcg tcactggctg
     1201 catgcggcta cgttgtacaa cattgccgcc tatcctcatc tgaaaggaga tgacctggcc
     1261 gagcaagcgc aggctttgtc aaaccgcgcc tatgaagagg ccgctcagcg tctaccgggc
     1321 acgatgcgac agatggagtt taccgtaccc ggcggtgcgc ccatcaccgg ctttttgcat
     1381 atgccgaaag gcgatggccc gttcccgacg gtattaatgt gtggtggtct ggatgcgatg
     1441 cagacggact attacagcct gtatgaacgt tattttgcgc cgcgcggcat tgcgatgctg
     1501 actattgata tgccgtcggt gggcttttct tcaaaatgga agctcaccca ggactcctgc
     1561 ctgttgcatc agcgcgtctt aaaggctctg cctaacgtac cgtgggtgga tcacactcgc
     1621 gtcgcggcct ttggtttccg tttcggcgct aacgttgccg tgcgtctggc atatcttgaa
     1681 tcgccgcgtc tgaaagcggt tgcctgtctt ggtccggtag ttcataccct gttgagtgat
     1741 tttaagtgcc agcaacaggt gccggaaatg tatcttgacg ttctggcgag tcgtttgggg
     1801 atgcatgatg cttccgacga agcgttgcgc gtggagctga atcgctattc attaaaagtg
     1861 caaggattgc tgggacgtcg atgcccaacg ccaatgttat caggctactg gaagaacgat
     1921 ccgttcagcc cggaagagga ctcacgctta atcacctcat catctgctga cggtaaatta
     1981 ttagagatcc catttaaccc ggtgtatcgg aattttgaca aaggtcttca ggaaatcacc
     2041 gactggatcg aaaaacgctt gtgttaaaaa tttgctaaat tttgccaatt tggtaaaaca
     2101 gttgcatcac aacaggagat agcaatgacg ttaccgagtg gacacccgaa gagcagattg
     2161 atcaaaaaat ttaccgtact aggcccgtat attcgtgaag gtaagtgcga agataatcga
     2221 ttctttttcg attgtctggc tgtatgcgtc aacgtgaaac cggcaccgga agtgcgtgag
     2281 ttctggggct ggtggatgga gcttgaagcg caggaatccc gttttaccta cagttaccag
     2341 tttggtctgt tcgataaagc aggcgactgg aagagtgttc cggtaaaaga tactgaagtg
     2401 gttgaacgac tggagcacac cctgcgtgag tttcacgaga agctgcgtga actgctgacg
     2461 acgctgaatc tgaagttgga accggcggat gattttcgtg acgagccggt gaagttaacg
     2521 gcgtgagtga aatgtgccgg atgcatcaca tccggcaata atcattaaaa ctgataggtc
     2581 atgccaaccg cgacaatatc atcattatta atattcaatt tgttatcgct atccagttgg
     2641 ttgattttat aatcaacaaa cgctgacata tttttgttga aataatatgt agccccgaca
     2701 tcgatataat tgaccagatc ttcatcaccg ataccttcaa tatctttccc tttcgataag
     2761 acataaccca gcgatggacg cagaccaaag tcaaactggt attgagcgac cgcttcaaag
     2821 ttctgtgtct tattggcaaa gccgccagtt attggcgtca ttttgcgtgt ttcagaataa
     2881 aaagttgcca gataaatatt attggcatcg tatttcagac ctgtagccca agcttctgca
     2941 cgcttgcctg tgccacggct ttgcaggttc tgctcgttgg tgcgatctga gttggtatag
     3001 gccccactaa tggcgaaatc gctgccgcca aagtcatatg tcaatgacgt gccgaagcca
     3061 tcgccgtttt gctttttaac gtcgcggttt tcgtttttcc cttgatattg cagggttaag
     3121 ttcaggccat cgataacgcc gaagaagtcg gtgttccgat acgtcgccag accgctggcg
     3181 cgtttggtca taaagttgtc ggtctgcgcc gaggagtcgc caccaaattc cgggaacata
     3241 tcggtccagg cttccacgtc atacaacgcg cccagg
//
LOCUS       DBFIFI010000573         1334 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.guided.423, whole genome shotgun sequence.
ACCESSION   DBFIFI010000573 DBFIFI010000000
VERSION     DBFIFI010000573.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.guided.423"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            6..965
                     /locus_tag="N7B39_005259"
     CDS             6..965
                     /locus_tag="N7B39_005259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HGU2424996.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     DSQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
     gene            977..1246
                     /locus_tag="N7B39_005260"
     CDS             977..1246
                     /locus_tag="N7B39_005260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2a subunit B"
                     /protein_id="HGU2424997.1"
                     /translation="MKKMFMAVLFALASVNAMAADCAKGKIEFSKYNEDDTFTVKVDG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
BASE COUNT          368 a          260 c          327 g          379 t
ORIGIN      
        1 tgtatatgaa gtgtatatta tttaaatggg tactgtgcct gttactgggc ttttcttcgg
       61 tatcctattc ccgggaattt acgatagact tttcgactca acaaagttat gtatcttcgt
      121 taaatagtat acggacagag atatcgaccc ctcttgaaca tatatctcag gggaccacat
      181 cggtgtctgt tattaaccac accccaccgg gcagttattt tgctgtggat atacgagggc
      241 ttgatgtcta tcaggcgcgt tttgaccatc ttcgtctgat tattgagcaa aataatttat
      301 atgtggccgg gttcgttaat acggcaacaa atactttcta ccgtttttca gattttacac
      361 atatatcagt gcccggtgtg acaacggttt ccatgacaac ggacagcagt tataccactc
      421 tgcaacgtgt cgcagcgctg gaacgttccg gaatgcaaat cagtcgtcac tcactggttt
      481 catcatatct ggcgttaatg gagttcagtg gtaatacaat gaccagagat gcatccagag
      541 cagttctgcg ttttgtcact gtcacagcag aagccttacg cttcaggcag atacagagag
      601 aatttcgtca ggcactgtct gaaactgctc ctgtgtatac gatgacgccg ggagacgtgg
      661 acctcactct gaactggggg cgaatcagca atgtgcttcc ggagtatcgg ggagaggatg
      721 gtgtcagagt ggggagaata tcctttaata atatatcggc gatactgggc actgtggccg
      781 ttatactgaa ttgtcatcat cagggggcgc gttctgttcg cgccgtgaat gaagatagtc
      841 aaccagaatg tcagataact ggcgacaggc ccgttataaa aataaacaat acattatggg
      901 aaagtaatac agctgcagcg tttctgaaca gaaagtcaca gtttttatat acaacgggta
      961 aataaaggag ttaagcatga agaagatgtt tatggcggtt ttatttgcat tagcttctgt
     1021 taatgcaatg gcggcggatt gtgctaaagg taaaattgag ttttccaagt ataatgagga
     1081 tgacacattt acagtgaagg ttgacgggaa agaatactgg accagtcgct ggaatctgca
     1141 accgttactg caaagtgctc agttgacagg aatgactgtc acaatcaaat ccagtacctg
     1201 tgaatcaggc tccggatttg ctgaagtgca gtttaataat gactgaggca taacctgatt
     1261 cgtggtatgt gggtaacaag tgtaatctgt gtcacaattc agtcagttga cagttgcctg
     1321 tcagactgag catt
//
LOCUS       DBFIFI010000574         1334 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.guided.424, whole genome shotgun sequence.
ACCESSION   DBFIFI010000574 DBFIFI010000000
VERSION     DBFIFI010000574.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.guided.424"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            6..965
                     /locus_tag="N7B39_005261"
     CDS             6..965
                     /locus_tag="N7B39_005261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HGU2424998.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     DSQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
     gene            977..1246
                     /locus_tag="N7B39_005262"
     CDS             977..1246
                     /locus_tag="N7B39_005262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2a subunit B"
                     /protein_id="HGU2424999.1"
                     /translation="MKKMFMAVLFALASVNAMAADCAKGKIEFSKYNEDDTFTVKVDG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
BASE COUNT          366 a          261 c          328 g          379 t
ORIGIN      
        1 tgtatatgaa gtgtatatta tttaaatggg tactgtgcct gttactgggt ttttcttcgg
       61 tatcctattc ccgggagttt acgatagact tttcgaccca acaaagttat gtctcttcgt
      121 taaatagtat acggacagag atatcgaccc ctcttgaaca tatatctcag gggaccacat
      181 cggtgtctgt tattaaccac accccaccgg gcagttattt tgctgtggat atacgagggc
      241 ttgatgtcta tcaggcgcgt tttgaccatc ttcgtctgat tattgagcaa aataatttat
      301 atgtggccgg gttcgttaat acggcaacaa atactttcta ccgtttttca gattttacac
      361 atatatcagt gcccggtgtg acaacggttt ccatgacaac ggacagcagt tataccactc
      421 tgcaacgtgt cgcagcgctg gaacgttccg gaatgcaaat cagtcgtcac tcactggttt
      481 catcatatct ggcgttaatg gagttcagtg gtaatacaat gaccagagat gcatccagag
      541 cagttctgcg ttttgtcact gtcacagcag aagccttacg cttcaggcag atacagagag
      601 aatttcgtca ggcactgtct gaaactgctc ctgtgtatac gatgacgccg ggagacgtgg
      661 acctcactct gaactggggg cgaatcagca atgtgcttcc ggagtatcgg ggagaggatg
      721 gtgtcagagt ggggagaata tcctttaata atatatcggc gatactgggc actgtggccg
      781 ttatactgaa ttgtcatcat cagggggcgc gttctgttcg cgccgtgaat gaagatagtc
      841 aaccagaatg tcagataact ggcgacaggc ccgttataaa aataaacaat acattatggg
      901 aaagtaatac agctgcagcg tttctgaaca gaaagtcaca gtttttatat acaacgggta
      961 aataaaggag ttaagcatga agaagatgtt tatggcggtt ttatttgcat tagcttctgt
     1021 taatgcaatg gcggcggatt gtgctaaagg taaaattgag ttttccaagt ataatgagga
     1081 tgacacattt acagtgaagg ttgacgggaa agaatactgg accagtcgct ggaatctgca
     1141 accgttactg caaagtgctc agttgacagg aatgactgtc acaatcaaat ccagtacctg
     1201 tgaatcaggc tccggatttg ctgaagtgca gtttaataat gactgaggca taacctgatt
     1261 cgtggtatgt gggtaacaag tgtaatctgt gtcacaattc agtcagttga cagttgcctg
     1321 tcagactgag catt
//
LOCUS       DBFIFI010000575         1329 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.guided.425, whole genome shotgun sequence.
ACCESSION   DBFIFI010000575 DBFIFI010000000
VERSION     DBFIFI010000575.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.guided.425"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..960
                     /locus_tag="N7B39_005263"
     CDS             <1..960
                     /locus_tag="N7B39_005263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HGU2425000.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     DSQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
     gene            972..1241
                     /locus_tag="N7B39_005264"
     CDS             972..1241
                     /locus_tag="N7B39_005264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2c subunit B"
                     /protein_id="HGU2425001.1"
                     /translation="MKKMFMAVLFALVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
BASE COUNT          369 a          260 c          324 g          376 t
ORIGIN      
        1 atgaagtgta tattatttaa atgggtactg tgcctgttac tgggcttttc ttcggtatcc
       61 tattcccggg aatttacgat agacttttcg actcaacaaa gttatgtatc ttcgttaaat
      121 agtatacgga cagagatatc gacccctctt gaacatatat ctcaggggac cacatcggtg
      181 tctgttatta accacacccc accgggcagt tattttgctg tggatatacg agggcttgat
      241 gtctatcagg cgcgttttga ccatcttcgt ctgattattg agcaaaataa tttatatgtg
      301 gccgggttcg ttaatacggc aacaaatact ttctaccgtt tttcagattt tacacatata
      361 tcagtgcccg gtgtgacaac ggtttccatg acaacggaca gcagttatac cactctgcaa
      421 cgtgtcgcag cgctggaacg ttccggaatg caaatcagtc gtcactcact ggtttcatca
      481 tatctggcgt taatggagtt cagtggtaat acaatgacca gagatgcatc cagagcagtt
      541 ctgcgttttg tcactgtcac agcagaagcc ttacgcttca ggcagataca gagagaattt
      601 cgtcaggcac tgtctgaaac tgctcctgtg tatacgatga cgccgggaga cgtggacctc
      661 actctgaact gggggcgaat cagcaatgtg cttccggagt atcggggaga ggatggtgtc
      721 agagtgggga gaatatcctt taataatata tcggcgatac tgggcactgt ggccgttata
      781 ctgaattgtc atcatcaggg ggcgcgttct gttcgcgccg tgaatgaaga tagtcaacca
      841 gaatgtcaga taactggcga caggcccgtt ataaaaataa acaatacatt atgggaaagt
      901 aatacagctg cagcgtttct gaacagaaag tcacagtttt tatatacaac gggtaaataa
      961 aggagttaag tatgaagaag atgtttatgg cggttttatt tgcattagtt tctgttaatg
     1021 caatggcggc ggattgcgct aaaggtaaaa ttgagttttc caagtataat gagaatgata
     1081 cattcacagt aaaagtggcc ggaaaagagt actggaccag tcgctggaat ctgcaaccgt
     1141 tactgcaaag tgctcagttg acaggaatga ctgtcacaat caaatccagt acctgtgaat
     1201 caggctccgg atttgctgaa gtgcagttta ataatgactg aggcataacc tgattcgtgg
     1261 tatgtgggta acaagtgtaa tctgtgtcac aattcagtca gttgacagtt gcctgtcaga
     1321 ctgagcatt
//
LOCUS       DBFIFI010000576         1329 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 105_162091
            SAMD00495977-rid25093873.guided.426, whole genome shotgun sequence.
ACCESSION   DBFIFI010000576 DBFIFI010000000
VERSION     DBFIFI010000576.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495977
            Sequence Read Archive: DRR381713
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424421.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424421.2
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:58:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,266
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,931
            CDSs (with protein)               :: 4,931
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 124 of 239
            Pseudo Genes (internal stop)      :: 59 of 239
            Pseudo Genes (multiple problems)  :: 33 of 239
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495977-rid25093873.guided.426"
                     /strain="105_162091"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-09-20"
     gene            <1..960
                     /locus_tag="N7B39_005265"
     CDS             <1..960
                     /locus_tag="N7B39_005265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HGU2425002.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     DSQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
     gene            972..1241
                     /locus_tag="N7B39_005266"
     CDS             972..1241
                     /locus_tag="N7B39_005266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2c subunit B"
                     /protein_id="HGU2425003.1"
                     /translation="MKKMFMAVLFALVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
BASE COUNT          367 a          261 c          325 g          376 t
ORIGIN      
        1 atgaagtgta tattatttaa atgggtactg tgcctgttac tgggtttttc ttcggtatcc
       61 tattcccggg agtttacgat agacttttcg acccaacaaa gttatgtctc ttcgttaaat
      121 agtatacgga cagagatatc gacccctctt gaacatatat ctcaggggac cacatcggtg
      181 tctgttatta accacacccc accgggcagt tattttgctg tggatatacg agggcttgat
      241 gtctatcagg cgcgttttga ccatcttcgt ctgattattg agcaaaataa tttatatgtg
      301 gccgggttcg ttaatacggc aacaaatact ttctaccgtt tttcagattt tacacatata
      361 tcagtgcccg gtgtgacaac ggtttccatg acaacggaca gcagttatac cactctgcaa
      421 cgtgtcgcag cgctggaacg ttccggaatg caaatcagtc gtcactcact ggtttcatca
      481 tatctggcgt taatggagtt cagtggtaat acaatgacca gagatgcatc cagagcagtt
      541 ctgcgttttg tcactgtcac agcagaagcc ttacgcttca ggcagataca gagagaattt
      601 cgtcaggcac tgtctgaaac tgctcctgtg tatacgatga cgccgggaga cgtggacctc
      661 actctgaact gggggcgaat cagcaatgtg cttccggagt atcggggaga ggatggtgtc
      721 agagtgggga gaatatcctt taataatata tcggcgatac tgggcactgt ggccgttata
      781 ctgaattgtc atcatcaggg ggcgcgttct gttcgcgccg tgaatgaaga tagtcaacca
      841 gaatgtcaga taactggcga caggcccgtt ataaaaataa acaatacatt atgggaaagt
      901 aatacagctg cagcgtttct gaacagaaag tcacagtttt tatatacaac gggtaaataa
      961 aggagttaag tatgaagaag atgtttatgg cggttttatt tgcattagtt tctgttaatg
     1021 caatggcggc ggattgcgct aaaggtaaaa ttgagttttc caagtataat gagaatgata
     1081 cattcacagt aaaagtggcc ggaaaagagt actggaccag tcgctggaat ctgcaaccgt
     1141 tactgcaaag tgctcagttg acaggaatga ctgtcacaat caaatccagt acctgtgaat
     1201 caggctccgg atttgctgaa gtgcagttta ataatgactg aggcataacc tgattcgtgg
     1261 tatgtgggta acaagtgtaa tctgtgtcac aattcagtca gttgacagtt gcctgtcaga
     1321 ctgagcatt
//
